LOCUS       DAOQJE010000001        14195 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_2067_length_14195_cov_6.133522, whole genome shotgun sequence.
ACCESSION   DAOQJE010000001 DAOQJE010000000
VERSION     DAOQJE010000001.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 14195)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14195)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14195
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2067_length_14195_cov_6.133522"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..478)
                     /locus_tag="PLT08_00005"
     CDS             complement(<1..478)
                     /locus_tag="PLT08_00005"
                     /inference="COORDINATES: protein motif:HMM:NF024304.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HNE02868.1"
                     /translation="MPREYIGQNPTAFQRRPHLTKDDAWIRENINTFKVGHFATSVDG
                     QPFINTSTFWYDEAEHQIVFHSNLAGRIRSNIEHNPKVSFEASVLGQLLPSNVALEFS
                     LQYRSIVAFGTAQLVTDPAEMRRLLYGLIGKYFSELSVGQEYREINDKELRATSVYA"
     gene            complement(488..1066)
                     /locus_tag="PLT08_00010"
     CDS             complement(488..1066)
                     /locus_tag="PLT08_00010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE02869.1"
                     /translation="MPRKPALNTDEILNTEYQYIAQNVFQSNEDRSRVTSFYFVSVGS
                     FVAAILGTRFEPDQSTISLAFFFLFTILTFMGALTIAQLARLRAAWHEAVEAMNQIKD
                     YYIKHMPELEPAFKWRKRQIPPTNKPYSIANLMAVEVALLSALTTGAGIYFLMTYIGI
                     SNWVTWSAVIVSVIFGYIAQWTWYRYLLVDNQ"
     gene            complement(1168..2403)
                     /locus_tag="PLT08_00015"
     CDS             complement(1168..2403)
                     /locus_tag="PLT08_00015"
                     /inference="COORDINATES: protein motif:HMM:NF014164.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HNE02870.1"
                     /translation="MNETFQTSLEKYADIIVNIGLNLHKEQRLNIAAGLPDAELVRLV
                     AKKAYQLGSPFVDVRWIDERLTRIRFENSKPEHLAEVPQWQIDREHEYGSRGDALLSF
                     ASQDPNLLEGISPDLIGRYRKAISEKYEPVAKYRNENTQTWCVVSTATPAWAQKVFPD
                     LPVEQAQEKLWEAIFNACRVNTADPVQAWREHAQKLHRQCDYLNEKRYAGLHYKAPGT
                     DLTLGLPDNHLWIGASGKTQNGIEYIPNLPTEEVFSMPHREKTEGYVTSSRPLILSGT
                     MIDEFTLTFENGRVSKVTAKQGEENIRKLLETDENASRFGEAALVPDSSPISQRKHLF
                     YNTLFDENASSHLALGTAYRYNVKGGTSMTKEEFIAAGGNDSLIHVDFMIGSDQMDID
                     GIRADGTREAVMRKGEWAF"
     gene            complement(2524..3786)
                     /locus_tag="PLT08_00020"
     CDS             complement(2524..3786)
                     /locus_tag="PLT08_00020"
                     /inference="COORDINATES: protein motif:HMM:NF014164.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HNE02871.1"
                     /translation="MTGTTHQQMLAKYAEAIVKVGLNIRAGQRLIITAAGTRGVPNQF
                     APLVREITKAAYGVGANYVDVIFADEEMMRIRAQHAPKGSFGEYSKWQIQGILDMINN
                     GDALLSIGGSNPDLLGGLDPEVVGQMQRAHLENWSPISEKVTTNAINWCVVAAAGEAW
                     AHKIFPDLPLNQAQEKLWQAIFETTRIDQPDPVGAWSKHIASLRERALYLQSKQYTAL
                     HYTAPGTDFTVGLPSGHLWISAQSMAQNGVAFTANMPTEEVFTLPDRNRADGVISATF
                     PLSYGGTLIENFQVHFENGRITKVTAKKGEAALQKLVDTDEGSHRLGEIALVPASSPI
                     GKRGHLFYNTLYDENASCHIAIGRAYRFTLAGGTELNDDEFTAAGGNISLNHVDFMIG
                     SPQMDIDGITKDGKHEPVMRQGEWAISL"
     gene            3945..5075
                     /locus_tag="PLT08_00025"
     CDS             3945..5075
                     /locus_tag="PLT08_00025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE02872.1"
                     /translation="MLVIEKVDTENKKQVKRFVELPPRLYADCPQWVPPLNVDAYNQL
                     NRKKHPFHEHSDVDFFLAVRDGLDVGRIAVIENKPFNQYHQEKTADFYLFECEDDLEA
                     ATALFNTAFEWAKSRGLTKMVGPKGMGPLDGYGVLVFGHEHRQTMTMLNYNHAYYQGL
                     VEAQGFTKEVDFVSCYLPADKFQMPERVKKISERVMQRGHLEIKTFKSKKDLLSWADR
                     IGTAYNNAFVNNWEYYPLSQREIDFVVENIMTIADHRLIKIIVHEQDVVGFLFAFHDV
                     SAAMQRAKGKLFPFGLLGILMELRRTNTVAVNGMGILPEFQGHGGNALLYYAMGDTLL
                     NFGQFTHVEMTQVAETTEQMRADLKNLNGVEYKNNRVYRKLL"
     gene            complement(5119..7401)
                     /locus_tag="PLT08_00030"
     CDS             complement(5119..7401)
                     /locus_tag="PLT08_00030"
                     /inference="COORDINATES: protein motif:HMM:NF024883.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing sensor histidine kinase"
                     /protein_id="HNE02873.1"
                     /translation="MSTSLAQVIVLLFIGVFYLPVIFFAIQRREEGQGAATWLVVLYA
                     LLAMVINIAEAIWQDRGDNSFLFQELQTYISFTLIIILMITIQVFLKRELWWAWLAYF
                     GVWVIGLALILTNALNLPDTLWTNGELILYRSRLGPAWVMLGWLILSIVMILNLFNAQ
                     HQSRQPLLRSRLGYWWAPVVFTLVNDILLFSGILIIGQPVRLLVAIGMAYVVGTHYVP
                     DLKNITRRAMIYVASTIGIVGFYIAGFLIIQALFGGNPSYSPLLAGAVVALFIALIFA
                     PLTDLVSRAINIWTKGDQYDASLTIHQYSESISNILDMDRLASIAIGIMLEAMQIERG
                     FLFLVDSERQADGRKTYKIRSARSPEERQLVAVELEEDGLIASYFIREQKPLLQYDLD
                     LLPAFRAASTVEKNWFTELRSEVYIPIFAKKQWIGLLAFGSKLSGNRYTREDLSVLSA
                     HANQTAVALENARLVDNLMRLNQELRQARRTLEKNNQELQRIDQAKSDFISIASHELR
                     TPLTVIKGYTEMLMDDPKLEKGQKSMMKGIHDGTIRLHEVMDSMFDIAQIDARSLKPH
                     LQPVDVGQLIHQVCLEHAKTVKEREQILTIKAQAIPLVKADSHLLEKLFHHLIRNAIK
                     FTPNNGWITITAKHIPAIINLPNGGIEIIVSDTGVGVDPNLREVIFSKFYQPGELGKH
                     STSKSRFKGNGAGLGLALSKGIIEAHGGRIWVESPGYDEVNFPGSQFHIILPLTKLEN
                     GEVQRTSEPLTIELGKAKEA"
     gene            7455..10421
                     /locus_tag="PLT08_00035"
     CDS             7455..10421
                     /locus_tag="PLT08_00035"
                     /inference="COORDINATES: protein motif:HMM:NF012356.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HNE02874.1"
                     /translation="MLTSRASRLKYNFSQDFFRPDGHVVFSDISSARAFANQMSALRT
                     APVSASDLYVISLLDEAYHILLRNYYSRYTGVMGRAMASLQSTFGSKYDLTLNKFTEE
                     FPPKAVFRGETTAGVYLATKLPNFGDFGHGVRAAAIEEMLLINNANKNPALAPYMDLF
                     DENVMNGSAFKDFTSHLLEFFSTQPGLGNGAAGKTETLAELLRGPIEASPDSLEGQLK
                     FILDKWGYLLGNEFSNRILRGMDYLREDVIRKQGPADTFNADVPVPTFTGHEYAEFER
                     YSPDQEWMPRLVLIAKNTYVWLDQLSKKYEREIKNLNDIPDEELDQLAARGFTGLWLI
                     GLWERSRASQRIKQRMGQEDAVASAYSLHSYDIANDLGGWNALQDLRSRAWQRGIRLS
                     ADMVPNHMGIDSQWVIEHPDWFLSLPFSPYPSYSFKSENLSSDLRVGIYLEDHYYDKT
                     DAAVVFQRRDLQTGDLRYIYHGNDGTSFPWNDTAQLDYSNPVVREAVIQVILHVARNF
                     PVIRFDAAMTLAKKHIQRLWFPEPGAGGAIPSRSQFGMPKAEFDEKIPIEFWREVVDR
                     VAAEVPDTLLLAEAFWLMEGYFVRTLGMHRVYNSAFMHMLRDEDNAKYRMAIKNTLEF
                     DPQILKRYVNFMNNPDEKTAVEQFGKGDKYFGVCTVLATLPGLPMFGHGQVEGYSEKY
                     GMEFRRPKWEETPDDGLIQAHDWRIFPLLHRRALFADVENFLLFDLQKPYSGLDENVF
                     AYSNRLDDSQSGAIERGLVIYHNKFGDTKGWIKTSTAALDKASGKMKRRSLAEGLALP
                     RRGYVIFKDYASQLEYVRSCEELWEKGMYVELGAYQCHAFMDFRFVHDTEWAIICNNL
                     KGAGVPSLQEEWRKTFAKVEEVKEEKPAKKKRTARKPAVKKTAAKSTKKAVTKKPAAK
                     KPKATVKKSATAKKPAKKMVTKKATASKPKAKKPTPKTTKTVKAKKTVTKKAPVKKTT
                     AKPVAKKKTVSKKKSK"
     gene            10477..10878
                     /locus_tag="PLT08_00040"
     CDS             10477..10878
                     /locus_tag="PLT08_00040"
                     /inference="COORDINATES: protein motif:HMM:NF021462.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2177 family protein"
                     /protein_id="HNE02875.1"
                     /translation="MKLKLYLITFLAFLGIDSLWLGLVAPSFYKSQIGYIMAENPNFF
                     AAGLFYLLFIFGMVVFIVEPGVREQTLMQAVARGALFGLVTYATYDLTNLATLEGWPI
                     LVTVVDLIWGTVLSAAVTLVSVWAGKRFIKA"
     gene            11149..11391
                     /locus_tag="PLT08_00045"
     CDS             11149..11391
                     /locus_tag="PLT08_00045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE02876.1"
                     /translation="MDYGMIGKLEKAKRYAEDRHRFRFNKFDLTFHGDNNEHHVGYDN
                     GVFSCDCEFFLTHKRCGHSMALEILLKDMIVETVEA"
     gene            11498..12577
                     /locus_tag="PLT08_00050"
     CDS             11498..12577
                     /locus_tag="PLT08_00050"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015679.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_function: GO:0071972 - peptidoglycan L,D-transpeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HNE02877.1"
                     /translation="MKRINRRDFLKLGGMTLGGLAFTPFLPGLTDFDDSFVVRIGTKD
                     MPVRKEPTDKSRIELSHYRDELVHVYGEITAEEPKHNPVWYRVWGGYLHRGRLHRVRT
                     IYQTPIDSIPEGTRLIADVSVPFTTPYRYSKTYGWQQMSPPLYYGSVHFIEAVEQGPE
                     MADYKGPWYRIFDELDSNVTYYAPAIHMRILPQDMLTPISPDVPYEDKLIEINLTSQM
                     LYAYEYGSLVFQTNISSGVPGDPTGGTGVPTTTPTGKFTILDKVPAKHMGYSYFGAQT
                     TGNVLADVDNYVLPGVPWTSFFTSQGHAFHGTYWHENFGSPMSHGCINMRTNEANWIF
                     RWARPEPIVGNVATRGLGTTVEIHY"
     gene            12636..13859
                     /locus_tag="PLT08_00055"
     CDS             12636..13859
                     /locus_tag="PLT08_00055"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013704.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HNE02878.1"
                     /translation="MPTLHWNGKHLSQPQPASLTLDSVLHPKGRGYPKSRTDGRVIQG
                     DNLAVMAALLPEYEGRINLIYADPPFFTNRQFHARVGRGEDSRKPSKWKLAEGYQDSW
                     TDMDSYLQFLYERLSLMHRLLAKNGTLYLHLDWHADSHARLILDEIFGAENFINEIIW
                     AYHGPSPIRTAFNRKHDTILMYAKSKEYIFNVDDVREPYNPNTVATFKASRKAGFGKI
                     PDLERGKVPEDWWYFPVVARLHNERTGYPTQKPEALLERIILASSNKNDLVADFFCGS
                     GTTALVAAKHGRKFITSDQSARAVHTARARLAETKSVFSMERDVMVKTEIATKSKKIK
                     VKLMGDRVLLQTSLDVDFWEVDPAWDGKIFRSVAQAQRHVRSGEIPMELKIKLGGKVC
                     IRVVTVEGKQFQLNI"
     gene            complement(13848..>14195)
                     /locus_tag="PLT08_00060"
     CDS             complement(13848..>14195)
                     /locus_tag="PLT08_00060"
                     /inference="COORDINATES: protein motif:HMM:NF012698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HNE02879.1"
                     /translation="QKLSNRIKPLLPGDGKNVLHVGPIRLDLEKRRVRCLGKSTKLTP
                     RLVTLLQLLMEKHGEVVEREPLFKKAWETNYTGDTRTLDVHISWLRRAIELDPDNPKF
                     LKTVRGVGYRLDI"
BASE COUNT         3644 a         3273 c         3526 g         3752 t
ORIGIN      
        1 tggcatacac cgacgtggct cgcagttctt tgtcgttgat ctcgcgatat tcctgtccaa
       61 cggacagttc agaaaaatat ttgccgatca acccatataa cagtctacgc atttctgccg
      121 gatcggttac aagctgggcg gttccaaaag cgacgatgct ccgatattgg agcgaaaact
      181 ctaatgcaac atttgaaggc agtaattgac caagaacact cgcctcaaaa ctgactttgg
      241 gattgtgctc aatattcgag cggattctcc ccgccagatt agagtggaat acgatctgat
      301 gttcagcttc gtcgtaccag aaggtggatg tattgatgaa gggctgtcca tccactgaag
      361 tggcaaagtg acccactttg aaagtattga tattttcgcg aatccacgca tcgtctttgg
      421 tgaggtgcgg tctgcgctga aaagcagtgg gattctgacc gatgtattct cttggcatga
      481 ggttctccta ttgattgtct acgagtagat agcgatacca ggtccactga gcgatgtagc
      541 caaagataac gctgacaatg actgctgacc atgtgaccca atttgaaatg ccgatatagg
      601 tcatgaggaa atagatgcca gcgccggtag tgagcgcgct gagcaaagcc acttccactg
      661 ccatgagatt ggcaatgcta tatggcttgt tggtgggtgg aatctgtcgc tttcgccatt
      721 tgaaggcggg ctccaactcg ggcatatgtt tgatgtaata atccttaatt tgattcatcg
      781 cttctactgc ttcatgccac gcggcgcgca gacgggcaag ctgagcaatg gtcaacgcgc
      841 ccatgaaagt tagaatggtg aagaggaaga aaaaagctaa agagatggtg ctttgatctg
      901 gctcgaagcg tgtgcccaga atggcggcaa caaaagagcc aaccgaaacg aagtaaaagg
      961 aggtcacccg cgagcggtct tcattcgatt gaaaaacgtt ctgggcaata tattgatatt
     1021 ctgtgttgag aatttcatcg gtgtttagtg cgggtttacg tggcatgcaa ttgctccttg
     1081 atgaaggtgc ttctattata taggcttatc acagatgcaa aaagcggacg tttcatcaac
     1141 gtccgctttt gttttcacgt attgcgctta gaaagcccat tcacctttac gcatgacagc
     1201 ttcgcgcgtt ccatcggccc ggatgccatc aatgtccatt tggtcagacc cgatcataaa
     1261 gtccacatgg atgaggctgt cattgccgcc agccgctatg aattcttctt tggtcattga
     1321 tgtgccgccc ttgacgttgt agcgataagc ggtacccaaa gcaaggtggc tggacgcatt
     1381 ttcatcgaag agggtgttat agaagaggtg ctttctctga gagatgggag agctatcagg
     1441 aaccaaagca gcctccccga agcgtgaggc gttttcgtct gtttctaaaa gtttgcggat
     1501 gttttcttcg ccttgtttag ctgtgacctt gctcacacgt ccattttcga aggtgagcgt
     1561 gaattcgtcg atcatggtac cagagagaat cagcggacgg ctggaagtga catagccttc
     1621 tgttttttca cgatgcggca tggaaaagac ttcttcggtc ggcaggttgg gaatgtattc
     1681 aataccgttt tgagttttgc cagaagcgcc gatccaaaga tggttgtctg gtaacccaag
     1741 cgttaggtct gtgccagggg ctttgtaatg gagcccagca tagcgcttct cgttcaagta
     1801 atcacattga cgatgaagtt tctgtgcgtg ttcgcgccat gcttgaacag gatctgcggt
     1861 attgacacgg caggcgttaa aaatggcttc ccataatttt tcttgtgctt gctccacggg
     1921 taagtctggg aaaacttttt gcgcccaagc aggggtggct gtagaaacaa cacaccaagt
     1981 ttgagtattt tcgtttctat atttggcaac aggctcgtat ttttctgaaa tcgcttttcg
     2041 atatctgccg atcaaatctg ggctgatgcc ttctagtaaa ttggggtctt gagaagcgaa
     2101 gctcaggagc gcatctccgc gagagccata ttcgtgctcg cggtcaattt gccactgtgg
     2161 aacttcagcg aggtgttcgg gcttcgaatt ttcaaaacgg atgcgcgtca acctttcatc
     2221 gatccagcgc acatcaacaa aaggagagcc tagttgataa gctttctttg ccactaaacg
     2281 aacaagctct gcatcaggta acccggctgc aatatttagg cgttgttctt tatgcaggtt
     2341 caaaccaatg tttacaatga tatcggcgta tttttcaaga gaggtttgga aggtctcgtt
     2401 catggtttct cctttagata gacagggcgt tgaatgattt ttaccataag tggactcaga
     2461 tgaccaccaa ctttggtgac ataaaaactc cccgcctcgt gacggggagt tgggttgatt
     2521 cggttacagt gagattgccc attcaccctg acgcatcacc ggctcatgtt tgccgtcttt
     2581 ggtaatgcca tcaatgtcca tttgcggaga accgatcatg aagtcaacgt ggttgaggct
     2641 gatgttgcct ccagccgcgg taaattcatc gtcgttcaac tcagttcctc cggcgagagt
     2701 gaagcggtag gcgcgtccaa tggcaatgtg acaggaggcg ttttcatcat aaagcgtgtt
     2761 gtagaagaga tgtccgcgtt tgccaatggg ggatgatgct gggacgaggg cgatttcacc
     2821 gaggcgatgt gaaccttcat cggtatccac taatttttga agggcggctt cacctttctt
     2881 ggctgtgacc ttggtaatac gcccattctc aaagtggact tggaagtttt cgatcaaagt
     2941 gccgccatag ctgagcggga aggtggcaga gatcacacca tctgcacggt tgcgatctgg
     3001 cagagtaaag acttcttcgg taggcatatt agcagtaaaa gcaacaccat tctgcgccat
     3061 cgattgggca ctgatccaga gatgtccgct gggcaagccg acggtaaagt cggtgccggg
     3121 ggcagtgtaa tgcaacgctg tatattgctt ggattgcaga tacaaagccc gttcgcgtaa
     3181 agaggcgatg tgtttgctcc atgcaccaac ggggtcgggt tggtcaatac gagttgtttc
     3241 aaagatggct tgccaaagtt tttcttgcgc ttggttcaga ggaaggtctg ggaaaatctt
     3301 atgcgcccat gcttcaccag cggcggcaac tacacaccag ttgatggcat tggtggtcac
     3361 tttttcacta atgggactcc aattttccaa atgggctcgt tgcatctgcc caacaacttc
     3421 agggtcaagc ccaccaagta ggtcggggtt tgagccgccg atggagagca aggcatcgcc
     3481 attgttgatc atgtccaaga tgccttgaat ttgccatttg gaatattctc caaagctgcc
     3541 tttcggggca tgttgagcgc ggatgcgcat catttcttca tctgcaaaga tgacatctac
     3601 atagtttgcg ccaacgccat acgcggcttt ggtaatttct cttactaatg gtgcaaattg
     3661 gttaggcaca ccgcgcgtcc ctgcggctgt gatgatcaaa cgttgcccgg cgcgaatatt
     3721 tagacctact tttacaatgg cttcggcata ttttgccagc atttgttggt gggtagttcc
     3781 tgtcataaat cctcagttaa tttgggagat agttacaagt atagcatggc gacaggggtt
     3841 tttgcacgag ggatatccat tacaaaagca atgcacaccc accttttaaa atggggtgga
     3901 agggatatac ttggctgaaa cctagcctct ataggagcaa aaccatgctt gttatcgaga
     3961 aagtggatac cgagaataaa aagcaggtca aacggttcgt ggagttacca ccccgcctct
     4021 atgcggattg cccgcagtgg gtgccgccat tgaacgtgga tgcgtacaat cagctcaatc
     4081 gaaagaagca tcccttccat gaacattctg atgtggattt tttcctagcg gttcgtgacg
     4141 gactggatgt agggcggatt gctgtaattg aaaacaaacc tttcaaccaa tatcatcaag
     4201 aaaagacggc agatttttat ttattcgagt gtgaggatga ccttgaagcg gctacggcat
     4261 tgttcaacac agcgtttgag tgggcaaaat cacgcggatt aaccaagatg gttggaccga
     4321 aagggatggg tccgttagat gggtatggag tgttggtttt cgggcatgaa caccgtcaga
     4381 ccatgaccat gctcaattac aaccatgctt attatcaggg attggttgaa gcgcagggtt
     4441 tcacaaaaga agtggacttt gtctcttgtt atttacccgc ggataaattc caaatgcctg
     4501 aacgagttaa aaagatctct gaacgtgtta tgcaacgggg tcatttagag ataaaaacat
     4561 tcaagagcaa aaaggacttg ttgagttggg cggatcgaat tggcaccgct tacaacaacg
     4621 cttttgtgaa taactgggag tattatccac tctcacaacg cgagatcgat tttgtggtgg
     4681 aaaatatcat gaccatcgcc gatcatcgcc tcatcaagat catcgtccat gaacaggatg
     4741 tggttggttt tctctttgcg tttcatgatg tttctgcagc catgcagcgt gcgaaaggca
     4801 agctctttcc gttcgggttg ttgggtattt tgatggaact tcgccgtaca aatactgtag
     4861 ctgtgaacgg tatgggaatc ctgccggagt ttcaggggca tggtgggaat gccttgttgt
     4921 attatgcgat gggagataca ttattaaact tcggtcaatt cacccatgtt gaaatgacac
     4981 aagtggctga gaccactgaa caaatgcgag cagatttaaa gaacttgaat ggggtggaat
     5041 ataaaaataa tcgtgtgtat cgcaaattac tgtaattctg ataaaaagag cggagattta
     5101 taaatctccg ctcttttttt atgcttcttt tgcttttcca agttctatgg tgagaggttc
     5161 acttgtgcgt tgcacttcgc cattttcaag cttggtcaac ggcaggatga tgtggaactg
     5221 actgcccggg aagttgacct cgtcgtagcc ggggctttcc acccagatac gaccaccatg
     5281 cgcttcaata ataccttttg agagagccaa ccctaaaccg gctccattac ccttgaagcg
     5341 tgatttgcta gtggaatgct tgcccagttc gcctggctga tagaacttcg agaagattac
     5401 ctcgcgcaag ttcgggtcta cgcccacacc agtatcgctg acgatgatct cgatgccacc
     5461 gttggggagg ttgatgatgg ctgggatgtg tttggcggtg atggtgatcc atccattgtt
     5521 tggcgtgaac ttgatggcat tgcggatcaa gtggtggaaa agcttttcca agaggtgcga
     5581 gtccgctttg acgagcggaa tggcttgggc tttgatggtt agaatctgtt cgcgttcttt
     5641 gacggtcttg gcatgttcca gacatacctg atggatcaat tgacccacat ccacgggctg
     5701 aagatggggc ttgagcgagc gcgcatcgat ctgtgcaatg tcgaacatcg agtccatcac
     5761 ttcgtgcaga cggatcgtcc catcgtgaat gcccttcatc atggacttct gacccttttc
     5821 tagctttggg tcatccatta gcatttcggt gtagcctttg atgacggtga gcggtgtacg
     5881 aagttcatga gatgcaatgc tgatgaaatc agattttgcc tggtcaatgc gttgtaattc
     5941 ttgattgttc ttttcaagcg tacggcgcgc ctgacgtaat tcttggttca agcgcatgag
     6001 attgtccacg aggcgggcgt tttcaagcgc aacggctgtc tggttggcat gtgcgctaag
     6061 aacggaaagg tcttctcggg tgtaacgatt gccgcttaat tttgaaccga atgccagcag
     6121 accgatccat tgttttttgg caaaaatggg aatgtaaact tctgaccgta attccgtaaa
     6181 ccaatttttc tcaacggtag aagcagctcg aaacgccggc aacaggtcca ggtcatattg
     6241 aagaagcggt ttttgctccc gaataaaata cgaagcgata agcccatctt cttccaattc
     6301 cacagcgacc aattgtcgtt cttcagggct acgtgccgaa cgaattttat acgtcttgcg
     6361 accatctgcc tgacgttcag aatcaacgag gaataggaag ccgcgttcaa tttgcatggc
     6421 ttccagcata atgccaatgg caatgctggc aaggcgatcc atgtccaaga tgttgctgat
     6481 gctttcacta tactggtgaa tggtgaggct ggcgtcgtat tgatcgcctt tcgtccagat
     6541 attgatggcg cgtgagacca agtctgtgag tggcgcgaag atcaacgcaa tgaaaagcgc
     6601 aacaacggcg cctgccaata gcggactata acttgggttg ccaccgaata aagcttggat
     6661 gatcaaaaag ccagcaatgt aaaagcctac aatgccgatc gtgctggcaa catagatcat
     6721 ggcacggcga gtgatgttct ttaggtctgg cacgtagtgt gtgcctacta cgtatgccat
     6781 gccaattgca accaacaagc gcaccggttg cccaatgata agaattccag agaaaagaag
     6841 aatgtcgttg acgagagtga aaacgacggg cgcccaccaa tatcccaatc ggctgcgaag
     6901 caacggctgg cggctttggt gttgcgcatt gaataagttt aggatcatca cgatggataa
     6961 aattaaccat cccaacatga cccaagcagg acccaaccgc gaacgataca ggatcaattc
     7021 accatttgtc cataaagtat ctggcaggtt taaagcattt gtaaggatca atgccagccc
     7081 aatgacccaa actccaaaat acgccagcca agcccaccat aattcgcgct tcaagaagac
     7141 ttgaatcgtg atcatcaaaa tgatgatcag ggtgaacgaa atatacgttt gaagttcttg
     7201 gaaaagaaaa gagttatccc cgcggtcttg ccaaatggct tcggcaatgt tgatcaccat
     7261 tgccagtaag gcatacaaaa caaccaacca agtggctgcg ccttgccctt cctcccggcg
     7321 ctgtatagca aaaaagatga ccggcagata gaacacgcca atgaacaata ggacgatgac
     7381 ttgggctagc gatgtgctca tgatgatgcc tgaaatttta ctctaattca ttattaatcg
     7441 gttatcatta ttgtatgcta acttcacgtg cctcccgtct caaatacaat ttttcgcaag
     7501 acttttttcg ccccgacggg catgttgttt tttcggatat ttcctccgct cgggcatttg
     7561 caaaccagat gtctgcgctt cgcactgccc ccgtttctgc ctctgacctc tatgtgattt
     7621 ctctgctcga cgaagcctat cacatcctac tccgcaatta ttacagccgt tacacgggtg
     7681 tgatggggcg ggcgatggct tccttgcagt ctacattcgg ctcgaaatat gaccttaccc
     7741 tgaataaatt taccgaagaa ttcccgccca aagcagtctt ccgcggtgag acgacggcag
     7801 gggtgtacct ggcaaccaag ttgccaaact ttggggattt tgggcatggt gttcgtgcgg
     7861 cagcaatcga agaaatgttg ttgatcaata acgccaacaa gaacccggca ctcgctccct
     7921 atatggattt gttcgatgaa aacgtgatga acggctctgc cttcaaagat ttcacgtcgc
     7981 accttttaga gttcttctcc actcagcctg gtttgggcaa tggcgcagcg gggaagactg
     8041 aaacccttgc cgaacttttg cgcggaccca ttgaagcctc cccagactca ttggaaggtc
     8101 agcttaaatt cattctcgat aaatgggggt atttgcttgg taatgagttt tcaaatcgta
     8161 ttttgcgcgg catggattat ttgcgcgaag atgttatccg caagcagggt ccagcagata
     8221 cgttcaatgc agatgttccc gtcccaacct ttaccgggca tgagtatgca gagtttgaac
     8281 gctacagccc agaccaagaa tggatgcccc gcctcgttct gatcgcgaag aatacctatg
     8341 tttggctcga tcaactttcc aaaaagtatg aacgtgagat taagaatctc aatgacatcc
     8401 cagatgaaga attagaccag cttgcagctc gcggatttac cggtttatgg ctcattggct
     8461 tatgggagcg cagccgcgcc tcacaacgta tcaaacagcg catgggtcag gaagatgctg
     8521 tcgcttcggc gtattctctt cattcgtatg atatcgccaa tgatcttggc ggttggaatg
     8581 cgttgcaaga tttgcgctca cgtgcttggc agcgtggcat tcgtctgtct gccgatatgg
     8641 ttccaaacca catgggcatc gactcgcagt gggtgatcga acatccagat tggtttttgt
     8701 ccctgccttt ttcgccctat ccttcctatt cgttcaagtc tgaaaatctt tcaagtgacc
     8761 tgcgtgtggg catttacctt gaagaccatt attacgataa gaccgatgct gcagtggtct
     8821 ttcaacgccg tgatttgcaa accggtgatt tgcgctacat ctaccacggc aatgacggta
     8881 cctccttccc atggaatgat acagctcaat tagattacag caatccggtc gttcgtgagg
     8941 cagtgattca ggttatcttg catgtcgcgc gcaacttccc ggtcatccgt tttgatgcgg
     9001 cgatgacgct tgccaagaaa catattcaac gtttgtggtt ccccgaaccc ggcgctggcg
     9061 gagccattcc atcccgttca cagtttggta tgcccaaagc agaatttgac gaaaagattc
     9121 caatcgaatt ctggcgtgaa gttgtggacc gcgtggctgc tgaggttcct gatacgcttt
     9181 tgctcgccga agccttctgg ttgatggaag gatatttcgt ccgcacactt ggtatgcatc
     9241 gtgtgtataa ctcggcgttc atgcacatgt tgcgtgatga agataatgcc aagtaccgca
     9301 tggcgatcaa aaatacactg gagtttgacc cgcaaattct taagcgttat gtcaacttca
     9361 tgaacaatcc cgatgaaaag accgcggttg agcaatttgg aaaaggggat aaatacttcg
     9421 gcgtttgtac agtgctggca accttgccgg gtctgcccat gtttgggcac ggacaagttg
     9481 aaggctattc tgaaaaatat ggcatggaat tccgccgccc gaaatgggaa gaaaccccag
     9541 atgacggttt gattcaagct catgattggc ggattttccc gctccttcac cgccgagcgc
     9601 tgtttgccga cgtggagaat ttcttactct ttgaccttca aaaaccctac agcggactcg
     9661 atgagaacgt ctttgcatat tccaaccgtt tggatgattc tcaatccggc gctatcgaac
     9721 gtggattggt tatctatcat aataagtttg gtgataccaa aggttggatc aaaacctcta
     9781 ccgccgcatt agacaaagct tctggcaaaa tgaagcgccg ctcgcttgcc gaagggcttg
     9841 ccttgccacg ccgcggatat gtcatcttca aagattacgc ttcacagttg gaatatgtcc
     9901 gctcgtgtga ggaactttgg gaaaaaggca tgtatgtgga attgggcgcg taccaatgcc
     9961 atgccttcat ggatttccgc tttgttcatg acacagaatg ggcgatcatt tgcaataact
    10021 tgaagggtgc cggtgtgcct tctcttcaag aagaatggcg caaaaccttt gccaaggttg
    10081 aagaagtcaa agaagagaaa cccgctaaga aaaaacgcac agcacgtaaa cctgccgtga
    10141 agaagacggc tgccaagtcc actaagaagg cagtgacaaa gaaaccagca gccaagaagc
    10201 caaaagctac cgtaaagaag tccgcgacgg caaagaaacc tgccaagaag atggttacaa
    10261 agaaagccac ggcgtcgaag ccaaaggcaa agaagccgac gcccaaaaca acaaagacag
    10321 taaaggcgaa gaaaacggta acgaaaaagg cgcccgtgaa aaaaacaact gccaaacctg
    10381 tggcaaagaa aaagacggtt agtaagaaga aaagcaagta gcccatctac tcggttagag
    10441 aggaattctc taaccgagta ttttttagga gacaccatga aactaaaact ctatctcatc
    10501 acattccttg cctttctcgg catagactcg ctttggcttg ggttggttgc gccgtcgttt
    10561 tataagtctc agatcgggta catcatggca gagaatccaa acttttttgc ggcgggattg
    10621 ttttatttgc tgttcatttt tggcatggtg gtctttatcg tggagccggg cgtccgtgag
    10681 cagacgttga tgcaggctgt agcacgcggt gcattgtttg gattggtgac ctatgccaca
    10741 tacgacctga ctaatcttgc gacattagaa ggctggccca ttttggtaac agtagtggac
    10801 ttgatctggg gaacggtctt aagcgcggcg gtaacgctgg tcagtgtttg ggcggggaag
    10861 aggtttatta aggcgtgaca agcccaagtc tagtgtgatg cgcgtagtca gattagggtg
    10921 tgatgaatat ttgtatatgt ccaccctaca aacgccatac gaaatcatta cattacgaaa
    10981 atcatattga cttgagtttt gaaaagtact atactgacaa acaataagag gtaacgctga
    11041 catgcgatta tttcacggaa atacccaggt ttgtaaataa agacacgcgt atatacacgc
    11101 gtgtcttttt tatatctaac ataatcaaac gaaaaggaga cttttgccat ggactacgga
    11161 atgatcggca agctcgagaa agcaaaacgc tacgcggagg atcgccatcg ttttcgcttc
    11221 aataaatttg atttgacctt tcacggggat aataatgaac accatgttgg gtacgataat
    11281 ggcgtctttt cttgcgattg cgaatttttc ttaacccaca aacgctgtgg gcattcaatg
    11341 gcgcttgaga tccttttgaa agacatgatc gttgaaacgg tcgaagctta accttacaat
    11401 tgaatagttt tgctgccctc cacatgcagt ggggggcagt ttttgtttaa gaaacattct
    11461 cattaaatct taaacaatgt agagtaaaat tttccttatg aagcgaatta accgtagaga
    11521 ttttctaaaa ctaggtggca tgacccttgg cgggctggct tttacgccat ttttgcccgg
    11581 tcttaccgat tttgacgata gttttgttgt gcgtattggc accaaagata tgcccgtgcg
    11641 taaagaacca accgataaaa gccggattga gctttctcat tatcgcgatg agttggtgca
    11701 tgtgtatggc gagatcactg ctgaagaacc gaaacacaac ccggtttggt atcgggtgtg
    11761 gggcggctac ctccaccgcg gacgtttgca ccgcgtgcgc acgatctacc agaccccgat
    11821 agactccatc cctgaaggta cacgtctgat cgcagatgtt tctgtgccat ttaccacacc
    11881 ctatcgctat tccaagacct atggttggca gcaaatgtct ccgccgttgt attatggctc
    11941 ggttcacttt attgaagccg tagaacaggg acccgagatg gcagattaca aaggtccttg
    12001 gtatcgcatt ttcgacgaac tcgactcaaa cgtcacctat tatgcacctg ccattcatat
    12061 gcgcatcctc ccgcaggata tgttgacgcc tatttctccc gatgtgccat acgaagataa
    12121 attgattgag atcaacctca catcgcaaat gttatatgct tatgaatacg gcagtttggt
    12181 cttccaaacc aatatttctt cgggtgttcc cggtgaccct acgggtggca caggtgtacc
    12241 cactacaaca ccgactggca aatttacaat tttggataaa gtgcctgcca agcatatggg
    12301 gtatagttac ttcggcgcgc aaacaacggg taacgtcctt gccgatgtag ataactacgt
    12361 cctccccggc gttccgtgga cttcattctt cacttcgcaa ggtcacgcct tccatggcac
    12421 ctattggcat gaaaattttg gctcgcctat gagccatggc tgtatcaaca tgcgcaccaa
    12481 cgaagccaat tggatcttcc gctgggcacg cccagaaccg attgtgggca atgtcgccac
    12541 tcgcgggctt ggcactactg ttgaaattca ttactaaaac ggtttgggat ttcttctccc
    12601 aaaccgaatc caccatttga ggtttccctg ctctcatgcc taccttacac tggaacggga
    12661 agcatctttc ccagccgcaa cctgcttcat taacccttga ctctgtcctc cacccgaaag
    12721 gacgtggcta ccccaaatcg cgtaccgacg gacgagtcat tcaaggggat aacttggctg
    12781 tgatggctgc tttgttgcct gaatatgaag ggcgtatcaa cctcatttac gccgacccac
    12841 catttttcac taacagacaa ttccatgccc gtgtggggag gggagaagac tctcgcaaac
    12901 cttccaagtg gaaacttgct gaaggctatc aagattcatg gacagatatg gactcgtacc
    12961 tgcaatttct gtacgaacgt ctcagtctca tgcatcgact gctcgccaaa aacggcacgt
    13021 tatatcttca ccttgactgg cacgctgatt cccatgcccg cctcatcctc gacgaaattt
    13081 ttggcgcaga gaacttcatc aatgaaatta tctgggcata tcatggacct tctccaatac
    13141 gaaccgcatt taatcgaaaa cacgacacca tcttgatgta tgccaaaagc aaagaatata
    13201 ttttcaatgt agatgatgtc cgcgaaccgt ataaccccaa taccgtagca accttcaagg
    13261 catctcgcaa ggcaggtttt ggcaagattc ctgatcttga acgtggcaaa gtgccagaag
    13321 attggtggta tttccccgtg gtggcacgcc tgcacaatga acgcacgggg tatcccactc
    13381 aaaagccaga agctttgctc gaacgcatta ttttggcatc ttcgaataaa aatgacctag
    13441 tagcagactt tttctgtggt tctggtacca ccgctcttgt ggcagccaag catggacgca
    13501 aatttatcac ctccgaccag tctgctcgag ctgtgcatac cgcgcgcgca cgattggctg
    13561 agaccaagtc tgtgttctcc atggaacgtg atgtgatggt caaaacagaa attgcgacca
    13621 aatcaaagaa gatcaaggtc aagttgatgg gagaccgagt ccttttgcaa acatctttgg
    13681 atgtggactt ctgggaagtg gatccagctt gggatgggaa aattttccgc agcgtagcac
    13741 aggctcaacg tcatgtgcga agcggggaaa ttccgatgga gttaaaaata aaactcggag
    13801 gcaaagtctg catccgagtc gtaacggttg aaggaaagca atttcaacta aatatctaaa
    13861 cgatagccaa cgccgcgcac ggtcttgaga aattttgggt tatcgggatc aagctcaatg
    13921 gcgcgccgca accaggagat atgcacatct aatgtgcggg tatcaccggt gtaattggtt
    13981 tcccatgctt ttttgaagag cggctcgcgt tccaccactt ctccatgttt ttccattaac
    14041 aattgaagca atgttaccaa gcgtggcgtg agttttgtgc tcttgcccaa acaacgcaca
    14101 cgtctttttt cgagatccaa acgaatggga ccgacatgta gaacattctt gccatcaccg
    14161 ggcaataaag gtttgatgcg gttagaaagt ttttg
//
LOCUS       DAOQJE010000002        13275 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_2285_length_13275_cov_5.247958, whole genome shotgun sequence.
ACCESSION   DAOQJE010000002 DAOQJE010000000
VERSION     DAOQJE010000002.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 13275)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13275)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13275
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2285_length_13275_cov_5.247958"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            114..2285
                     /locus_tag="PLT08_00065"
     CDS             114..2285
                     /locus_tag="PLT08_00065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE02880.1"
                     /translation="MVKINIALPKILVSTPKYAWEWNSVTDTFWLRDNNQRIISSAHH
                     KPLCLTSPAQVEFITSTYEIKDDQVKITYHGQNPASSLTVIWSFHSDFISLEPFLLTL
                     PADMDLVRIIYFPVADGTSYKPSMYSHYAVVPGLSMSTNISPIVDLHSRLNTTAVLGS
                     GAMRGPGLTQQWGLPAHYFCTFNTSDRWNAIEAKAQQSEAACWGLAALPQGDFRLEMR
                     EIGISPVLNLRTDLWKHTPDTFGFHFLVTFGENYHEAIRTYYQVLQREKFIRPKINSA
                     KKKEVLFAPQYNTWGVESALALHPEQLTEDLVRDIFSKLQRSGIQANTFVIDDKWEGM
                     HGELKHDPERFPYFENLLREIRAQGYYIGLWAAFLRCQNPAALGLDESHLLQTHEGKT
                     LRLDHQTSHYGIFDMTQPKVQDVLRQRAKEFIRCYQPDLIKFDFGYELPSLDVAAPKD
                     MTFAGERLLQKGLEVIVGAMKEENPDLVIMYYGLSPLLIDHYDLHSPDDLVYCIGDYD
                     LETNRRVFFSSLCGEMGMPTYSSSGYDWDSAIDIWFDSVASGSLGSLHCFDGDENGEK
                     PSAKHIAKFNGINSLVRKEAHFTVKPLHAKWQGGLRAGFSPSWERIENGKTVLLALRT
                     HQFDGKPIPNSYKDVIAFSMMMVIATLDNESIGEATRIGIVPFGDGHCSIKSKYHQAI
                     LIEHHLGGKRNTSRLTFADQLTLNLVQHESLEWIEIIFENE"
     gene            complement(2315..3514)
                     /locus_tag="PLT08_00070"
     CDS             complement(2315..3514)
                     /locus_tag="PLT08_00070"
                     /inference="COORDINATES: protein motif:HMM:NF024110.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNE02881.1"
                     /translation="MKKIFAIAWKDAIVRFASSSELLFFIILPLVFTFLLAGGTPKAD
                     GGDTRIRLVVVDQAETTISKNIIAELESSTAVRPEILSLEEAESQFDSRRASVVLILP
                     DGLDLQAIQDGTAQVDFRQQPNNLNATVAERAVQTAIRQVSSAVNAANLAVNDAEKQK
                     PFASEADRQSYFDASLKLAQSIQADAPERVTIIEGSTSFEVDYDPRANASAGQLVTWV
                     FIPLFGISALFAYERQQGTLRRILTTPTSKATYLLGTIAGQVVMALIQMSLLILFSIF
                     AMKLNWGRDLAALFIILTTAALAAGAIGTAMGTFIKTEGQASGLSIMLGMVMALLGGC
                     WYPLELFPPVMQTIVKILPTTWAMQGLLDLVLRNGGLIQILPEAGVLLGFAAIFFSVG
                     VLRFRYE"
     gene            complement(3511..4779)
                     /locus_tag="PLT08_00075"
     CDS             complement(3511..4779)
                     /locus_tag="PLT08_00075"
                     /inference="COORDINATES: protein motif:HMM:NF024110.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNE02882.1"
                     /translation="MLKTFLIGFKDVRLAFRDRAALLLMLAAPFVLTVGLGLVTGRFG
                     GNTGSGISDIPVITVNLDQEQLGNALADVFNSAELADLLEPATGSDPEAARQLIDEDK
                     AAAVVIIPEGFTQSIIPQQSDFTAGNTITTDAVKIEVYANPARSTGAGIVKAIVDEFL
                     SRVEEGRISKTTSILQLLTSQKITPQQAIAAGEAASTGVQDNVPESLAIKINSSTAGG
                     EAVKFDALAYIAPGMALMFLMYTVSYGGRSILAERAQGTLPRLLVSPTSPSQILGGKV
                     FGIFLTGAAQMLILIGASSLLFQLNWGDPIGVILLVLAAVFGATGWGMIITALARTPG
                     QVASIGTAIMLIFGIMGGSFINLEQMPPFVQTFSKITPNAWALDGFTTLALGGTLTDL
                     SAPILALLAMGSILFLVSVALFGKKNLVQK"
     gene            complement(4874..5818)
                     /locus_tag="PLT08_00080"
     CDS             complement(4874..5818)
                     /locus_tag="PLT08_00080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNE02883.1"
                     /translation="MSAILQVQNLVKNYGDFQAVKGVSFNVEEGEIFSLLGPNGAGKT
                     TTISMLSTLYAPTSGDATIGGFSITKDPMGVRGIIGVVPQDLALYEDLTARENLMFWG
                     QMYNLSGKPLAMRVDEVLEQIGLTDKAKDRLKTYSGGMKRRVNIGVGLLHKPRLLFMD
                     EPTVGIDPQSRRAILDTVKDLNKQGMTLLYTTHYMEEAEELSNRIGIFDHGELIALGT
                     QKELTQNVGEAETLVLHVGENDDSETLASVLKKIQGVQQATAVDHEVSVVCAEAEDVL
                     AAVVTTANDRGIKIRSIDIREPNLEAVFLHLTGRALRD"
     gene            6020..8749
                     /locus_tag="PLT08_00085"
     CDS             6020..8749
                     /locus_tag="PLT08_00085"
                     /inference="COORDINATES: protein motif:HMM:NF037660.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HNE02884.1"
                     /translation="MIYNLIMVTPILATKLFIPPQPSRVVLRSRLVKQITDGLSTGHK
                     LTLISAPAGFGKSTLASEMLAVCGQPAAWLSLDENDNDLARFLVYFISALQSISPNLG
                     AEILNIIQTQQLPPIDSLLTALLNEIATLTNDFILVLDDYHLVDSKSVDEALTFFIDH
                     LPPQMHLVITTREDPSLPIPRLRARNQLTEIRAADLRFTAAEAAEFLNQVMSLELSVE
                     EIASLENRTEGWIAGLQLAALSMKGQQDIHGFIQAFAGDHRYIVDYLAEEVLRRQTEE
                     IRGFLLQTSILERLNGSLCDAVTAQLGSKSKLEQLQRGNLFLIPLDDRREWYRYHHLF
                     ADVLRMHLAAEQPEAVPVLHCRASEWFEQNNMTADAIRHALVGEDFERAAGLIEKVLP
                     IMRQSRQEPTLLKWLKALPIKLFQHRPVLNVHYIGMLLQNGQIDGVESRLRDVEQWLA
                     APEDIRRGSIYVDEVEFQRLPISVAMYHSAMALAQGDVVNAMKYASKVLELSREDDDF
                     PRGAASSLMGLASWTNGNLEIAYEMFSKGMAHLQKVEYVSDVIGGSVTLADIRITQGR
                     LRDAMSIYERGLQLATKSGIPTLRGAADMYVGMAELHREHNDLQAAMQLLEKSRELGE
                     FNGLPKNPYRLRVAMAGLEEAQGNLNNALNLLDEAEPRFVADFSPNVRPIQALKVRVW
                     IKQGELEKALAWAHERKLSIKEEPSYLREFEQIMFARTLLLQGDDTDSLLIEYLERLL
                     KAAEAGGRNGSVIEILILQALAYQMQEDIPVALSSLERALTLAEPEGYIRMFLDEGAN
                     MEMLLREAAAHKITPNYTGKLLAAFEAERRGLVVETPPSAVPVSGSLIEALSQRELDI
                     LRLFKTELSGPEIAQELVIALSTVRTHTKSIYNKLNVNSRRAAVKRAIELGLI"
     gene            8945..9610
                     /locus_tag="PLT08_00090"
     CDS             8945..9610
                     /locus_tag="PLT08_00090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE02885.1"
                     /translation="MKVTNTKLIRWAGLSAVIAGIIFTAIQPTHPSINTSAFIIITSL
                     KTSISIFGLLGITGLYARQVEETGWKGLAGYLLLTIYYAVQMCISFIEPTVLPLLTTT
                     APTFVESMLQLSSGTGVPMNLGSLTNVYSLVSILYVLGSMLFGMAMFRVRILPRGAAA
                     LFAVSGPLAGTMFTLLPYHLVQLTSIPIGAAMVWLGYALFSERREKKSASLLDQGIVS
                     QVA"
     gene            9641..10324
                     /locus_tag="PLT08_00095"
     CDS             9641..10324
                     /locus_tag="PLT08_00095"
                     /inference="COORDINATES: protein motif:HMM:NF021576.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2306 domain-containing protein"
                     /protein_id="HNE02886.1"
                     /translation="MKAIIQNNSQIKAPARKTQTPWWVPAGLVLLSVIPIIFGTLRLN
                     QLINGAEITPGNARFFASPAPVVIHIISAVVYALLGAFQFMSRLWTRGIKWHRWVGRF
                     LVPVGLLVGLSGLWMTLFYDYPEGASKLLYGLRLFFGSGMVLSIIIGFISIRRRDINQ
                     HQAWMTRAYAIGMGAATQVLTGIVGALTLGEVNEFENALLMGAGWIINLVIAELSIRK
                     SQTRQTRTA"
     gene            10376..11287
                     /locus_tag="PLT08_00100"
     CDS             10376..11287
                     /locus_tag="PLT08_00100"
                     /inference="COORDINATES: protein motif:HMM:NF024989.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent alcohol dehydrogenase"
                     /protein_id="HNE02887.1"
                     /translation="MKAIVATQYGGPEVLQLKEVQKPTPKDNEILVKVHATTVTAADF
                     RMRSFTVPVAVWIPARLALGITKPRQPIFGSELAGVVEAVGKDVTRFKVGDQIFASTL
                     TENFGGYAEYKSLPEKAMMAIKPTNLTYEEAAALPIGATTALRLLRKGNIQRGQKVLI
                     YGASGSVGTYAVQLAKYFGAEVTAVSSTSNLELVKSLGADHVMDYTKGDLTSGAERYD
                     VIFDTVAKFPKSQYSNILAENGSFVSMAKLDSKESMDNLVFIKDLIEKGEIKAVIDRC
                     YPLEDMVEAHRYVDTGRKKGNVVITVP"
     gene            11287..11550
                     /locus_tag="PLT08_00105"
     CDS             11287..11550
                     /locus_tag="PLT08_00105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE02888.1"
                     /translation="MPNGQNLENDPDRPKIFRIRIQGHLGQQWTNWFDGLTITLEEDG
                     NTLLAGLVTDQSALHGVLKKIRDLGMPLLSVHAIDPDEQTGNS"
     gene            11547..12044
                     /locus_tag="PLT08_00110"
     CDS             11547..12044
                     /locus_tag="PLT08_00110"
                     /inference="COORDINATES: protein motif:HMM:NF022606.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2938 domain-containing protein"
                     /protein_id="HNE02889.1"
                     /translation="MNDLFLSPVSTMIVGLGATLTFDLWGLFLKYVFKIAPSDFCLVG
                     RWVLYMPKGIFKHRNISATPRKSTECVVGWFTHYLTGILFAIAFVAQTGNGWLQHPTP
                     TPALTFGVVTVSIPFFIMQPAFGLGVAASKTAKPMQARVRSLVNHLVFGLGLFIFGLL
                     ASWLI"
     gene            complement(12203..>13275)
                     /locus_tag="PLT08_00115"
     CDS             complement(12203..>13275)
                     /locus_tag="PLT08_00115"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013227.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HNE02890.1"
                     /translation="NGTITMEVALRAAGIGWGDEVIVPAYTFQATASAPMGAGAIPVI
                     VDVDPNTYCLDAKAAEKAITPKTKAIIPVHLGSNMADMDAIMALAEKHDLIVIEDCAH
                     AHGAKWRGMGAGTIGHFGSFSLQSSKTLTSGEGGILLCKSAEYAALAASVIDCGRPHA
                     LGGGGEDPNGLSVQGGNFRISELQAAMALTGIERFPEQARQREEMAAYMDEALSEIRG
                     VRVLKRDERHTTRSFYRYIFAIDPQEFGVEHDLLCGALDAEGVDCWTGYNAMHKYDLF
                     QPQKSRLAVPNAFPQYFDFKSMELPEATRACEHEAVWLDESAFRAGTKGVDDVVSAIK
                     KIQRNAGELNAAAEDLRRKYGR"
BASE COUNT         3262 a         3520 c         3267 g         3226 t
ORIGIN      
        1 taggtcgatt aggagtcatt tgatataatg tgaccggtca cgtgaacggt aacatcagtt
       61 tacaagaaca aagttccttt gtcaagtagg tattgaaaaa ttgaggtgtt ttgatggtga
      121 aaattaatat cgccctcccc aaaattctag tctccacccc aaaatacgca tgggaatgga
      181 atagcgtcac ggatacattt tggctacgcg acaataatca acgcattatt tccagcgctc
      241 atcacaaacc gctctgcctc acctcacccg cccaggtgga atttataacc tcgacatacg
      301 aaattaagga tgaccaagta aaaatcacct accatggaca aaaccccgct tcttccttaa
      361 ctgtaatctg gtccttccac tcagacttta tctccctcga accgttcctc ctaacccttc
      421 ctgcagacat ggaccttgtc cgtatcatct acttccccgt agcggacggt acctcctaca
      481 aaccatctat gtacagccac tatgctgttg tccccggctt gagcatgagc accaacatct
      541 cacccatagt ggatttgcac tcacgcctta acaccactgc ggtactcggc tccggtgcca
      601 tgcgcggacc cggtctcacc cagcaatggg gattgcccgc ccattatttc tgcaccttta
      661 acacctccga ccgctggaat gccattgagg caaaggcaca acaaagtgaa gcggcttgct
      721 ggggtctcgc tgcattacct caaggcgact tccgtttgga gatgcgtgag atcggcatta
      781 gtcccgtcct caacctgcgt actgatttat ggaagcacac acctgataca tttggctttc
      841 attttctcgt cacctttggc gagaattatc atgaagccat ccgcacctat taccaggtct
      901 tgcagcgtga aaaattcatc aggccgaaga taaattctgc gaaaaagaaa gaagtgctgt
      961 tcgcgccgca atataacacc tggggtgttg agtctgcttt ggctctgcac cctgagcaat
     1021 tgaccgaaga cctcgtccgc gatattttca gcaaacttca acgctctggc atacaagcaa
     1081 atacctttgt cattgatgac aaatgggaag gtatgcacgg tgaactcaaa catgaccccg
     1141 aacgctttcc ctactttgaa aatctcttaa gagaaatccg tgctcaaggc tactacatcg
     1201 gtttatgggc ggcgtttttg cgctgtcaaa atcccgccgc gctcggcttg gatgagtcac
     1261 acttattaca aacacatgaa ggcaaaacgc tccggctcga ccatcaaacc tcacattatg
     1321 gcatcttcga tatgacccag cccaaagtgc aggatgtgtt acgccaacgt gccaaagaat
     1381 tcatccgctg ctaccaacct gacctgatca aatttgactt cggatatgaa ttaccctccc
     1441 ttgatgtcgc cgcaccaaaa gatatgactt ttgcaggcga acgcttgctg caaaaaggtt
     1501 tggaagtcat cgtgggtgca atgaaggaag aaaaccccga cctcgtcatc atgtactatg
     1561 gtctctctcc cctgctcatc gatcactacg acctgcatag ccccgatgac ctcgtctact
     1621 gcattggcga ttacgatctt gaaaccaatc gccgcgtctt cttcagcagc ctgtgcggtg
     1681 agatgggtat gccaacatac agttcctctg gttatgattg ggactctgcc atcgatatct
     1741 ggtttgactc ggtcgcttcg ggcagcctcg gctcattgca ttgtttcgat ggcgatgaaa
     1801 atggggaaaa accgtccgcg aagcacattg ccaaatttaa tgggatcaac tccctcgtca
     1861 ggaaagaggc gcatttcact gtcaagcctt tacatgcaaa atggcagggc ggtttgcggg
     1921 caggctttag tccatcatgg gagcgcatcg aaaacggcaa gacggtccta ttggcattgc
     1981 gcacacatca attcgacggc aagccgatac caaacagtta taaagatgtt attgcattca
     2041 gcatgatgat ggtgatcgcc acgctcgata atgaaagcat cggcgaagcg actcgcattg
     2101 gtatcgtgcc attcggcgat ggacactgca gcatcaaaag caaataccat caggcgatcc
     2161 tcatcgaaca tcatctaggc ggaaaacgaa acacatcgcg tctcacattc gccgatcaac
     2221 taacgctcaa cctcgttcaa catgaaagcc tcgaatggat tgaaattatt tttgaaaatg
     2281 aatgactatg ccttaaacct tttgcccgtc agctctactc gtatcgaaac cgcagcacgc
     2341 cgaccgaaaa gaagatcgcc gcgaacccaa gcaatactcc cgcctctggg agaatctgga
     2401 tcaacccgcc gttgcgcagg accagatcca acagtccttg catggcccag gtggttggca
     2461 atattttgac gatggtttgc ataacaggcg gaaacaactc caacggatac cagcatccac
     2521 ccaacagcgc catgaccatg ccaagcataa tgctgagtcc actggcttga ccttcggtct
     2581 tgatgaaagt acccatcgcg gttccgatcg ctcctgcggc taaggcggcg gtggttagga
     2641 tgataaacag ggcagccagg tcacgtcccc aatttaattt catggcaaaa atactgaaca
     2701 agatcaaaag ggacatttgg atcaacgcca tcaccacctg acctgcgatc gtcccaagca
     2761 aataggtggc tttgctggtt ggggtggtca ggatgcggcg caaagtccct tgctgtcttt
     2821 cgtaggcaaa cagcgcagag atcccaaaca atggaataaa cacccatgtg acaagttgtc
     2881 ctgccgatgc gtttgcacgc gggtcgtaat ccacttcaaa cgatgtggaa ccttcaatga
     2941 tcgtcacgcg ttctggcgca tctgcctgaa tggattgggc aagtttcaaa gacgcgtcga
     3001 aataagactg gcggtctgct tcagacgcaa acggcttctg cttctctgcg tcattcaccg
     3061 ccaaatttgc cgcattgacc gcactgctga cctgtcgaat ggcggtctgc acggcacgtt
     3121 ccgcgacggt ggcgtttaaa ttattcggct gctgacgaaa atcaacctgc gccgttccat
     3181 cttgaatggc ttgcaggtcc agcccatcgg ggaggattaa tacaaccgac gcgcggcggg
     3241 aatcgaactg gctttcggct tcttcaagag aaaggatttc tggtcgcacg gctgtggaac
     3301 tttccaactc ggcgatgata ttttttgaga tggtcgtttc ggcttgatcc acgacgacga
     3361 gtctgatgcg ggtatcgccg ccatctgctt tgggtgttcc gcctgccaga agaaaagtaa
     3421 aaacgagcgg caggatgata aagaagagca actccgatga actcgcaaat cggacaatgg
     3481 catccttcca ggcgatggca aatatctttt tcatttctgc accagattct tttttccgaa
     3541 gagtgcgaca gaaaccaaaa acaagatact gcccattgca agcagggcaa gtatcggcgc
     3601 ggaaagatcg gtcaatgtcc cgccaagcgc caatgtggtg aatccatcca atgcccaggc
     3661 attcggcgtg atcttgctaa acgtctgcac gaagggcggc atttgttcaa ggttgataaa
     3721 acttccgccc atgattccaa agatcaacat gatggctgtg ccgatgcttg ccacctgacc
     3781 tggcgtgcgc gcgagggcag tgatgatcat gccccaacct gtggcgccga aaacagccgc
     3841 gagcaccagc aggatgacgc cgatggggtc gccccaatta agttgaaaga gcaacgacga
     3901 tgcgccgatg agaattaaca tttgtgccgc gccagtcagg aagatgccaa aaacttttcc
     3961 gcccaaaatt tgtgacgggc ttgttggtga aacaagcagg cgcggcaggg ttccctgtgc
     4021 gcgctccgcc aggatcgaac gcccgccgta actgacggtg tacattagga acatcaatgc
     4081 catgccaggc gcaatgtatg ccagcgcgtc gaacttgacc gcctcaccgc ctgccgtgga
     4141 tgaatttatt ttgatggcaa gcgactcggg tacgttgtct tgcacacctg tactcgctgc
     4201 ttcccctgcg gcgatcgcct gttgcggcgt gatcttctgg ctggtcagaa gttgcaggat
     4261 cgaagttgtt ttgctgatgc gcccttcttc cacacggctg agaaattcat ccacgatggc
     4321 tttgacaatg cccgcgcctg tcgaacgcgc tgggttggca tacacttcaa tttttactgc
     4381 gtccgttgtg atggtgttcc cagcagtgaa gtcactttgc tgcgggatga tggattgagt
     4441 gaagccttcg gggatgatga cgacagccgc ggctttgtct tcatcaatta attggcgggc
     4501 cgcttcaggg tcggacccgg tggctggttc cagcaaatca gcaagttctg ccgagttgaa
     4561 gacatccgcc aacgcgttgc ccaactgctc ttgatccaaa ttgacggtga tgacgggaat
     4621 gtccgaaatc ccgctgccag tgtttccgcc gaatcgtccc gtgaccagac ccagccccac
     4681 cgtcagcacg aacggagcgg cgagcattaa taaaagcgcg gcgcggtcac gaaatgcaag
     4741 gcgtacatct ttgaacccga taaggaaagt ttttaacatg attttgtgcc tcaccacaaa
     4801 gtatcacaaa ggtacacaaa ggttttaaat ctttttcctt tgtgatactt tgtgcgcctt
     4861 cgtggtaaaa gttttagtcg cgcagggcgc gtcccgttag atgcaaaaac accgcttcaa
     4921 gattcggctc acggatatca atggagcgga tcttgatacc gcgatcattt gccgtggtga
     4981 ccaccgccgc cagcacatct tcggcttcgg cgcagaccac cgagacttca tggtcaactg
     5041 cggtggcttg ctgcacccct tgaatttttt tgagtacact ggcaagtgtt tctgagtcat
     5101 cattttcgcc aacgtggaga accagcgttt ctgcctctcc cacattttgc gtcagttcct
     5161 tttgcgttcc gagcgcgatc aactcgccgt ggtcaaatat gccaatgcgg ttggaaagtt
     5221 cctccgcttc ttccatgtaa tgcgtggtgt agagcagagt catgccctgt ttgttgaggt
     5281 ctttcaccgt gtccaaaatt gcgcggcgtg attgcgggtc gatcccaaca gtgggctcat
     5341 ccatgaacaa cagacgcggc ttgtgcagca atcccacacc gatattgaca cgccgcttca
     5401 ttccgcctga ataagtcttg agcctgtctt tggctttatc ggtcaagccg atctgttcaa
     5461 ggacttcgtc cacgcgcatg gcaagcggct tgccgctcaa gttgtacatc tgcccccaga
     5521 acatcaaatt ttcgcgggca gtcaggtctt catacaacgc caggtcttgc ggaacaacac
     5581 cgatgatgcc gcgtacgccc attgggtctt tcgtgatcga aaatccgccg atggtcgcat
     5641 cgcctgaggt gggcgcatat aaagttgata acatggaaat ggtggtggtc tttcccgcgc
     5701 cgttcggacc cagcaggctg aaaatttcac cctcctccac attgaacgac acgcctttta
     5761 cggcttgaaa atcgccgtag tttttaacaa ggttctggac ttgcaagatc gcgctcatgt
     5821 tttctcctca tgcaaatgtc taacccaact gcctgccatt gaaagttgtc ttttcagttg
     5881 tgagccagtc gaaaatcaaa gttcccatcc aaagactcga atctaagaat caaaaaccac
     5941 atgtggttat tatatatcgg aatattttgg ggaaaacagg tcaggtcgaa agatgggcaa
     6001 atgaaattca aagatttaga tgatatataa tctaattatg gtcactccca ttctggctac
     6061 gaaacttttt atcccgccgc agccttccag ggttgtcctc cgctcccgcc tggtcaaaca
     6121 aattacagat ggattatcta cggggcacaa gttaaccttg atctctgctc ctgctggatt
     6181 cggcaagagc accttagcca gcgaaatgct cgcggtttgc gggcaacctg ctgcatggtt
     6241 gtcgctcgac gagaatgaca atgaccttgc gcggtttctg gtctatttta ttagcgcatt
     6301 acagagcatt tcgccaaacc tgggggcaga gatattgaac attattcaaa ctcaacaatt
     6361 gccgcccatt gactcccttc tcacagcatt gctcaatgag atcgcgaccc tcacaaacga
     6421 tttcatcctt gtcctggatg actaccatct tgttgattcc aaatcggttg atgaggcgct
     6481 cacctttttc attgaccact taccgccgca aatgcatctg gtcatcacca cgcgtgaaga
     6541 tccatccctg cccattccgc gcctgcgtgc ccgaaaccag ttgaccgaga tccgcgcggc
     6601 agatttgcgt tttaccgctg ccgaagccgc ggaatttctc aatcaggtga tgagccttga
     6661 actcagcgtt gaagaaattg catcgcttga aaatcgcact gaaggctgga ttgctggtct
     6721 gcaactcgcc gcgctttcaa tgaaggggca acaggatatt cacggattta ttcaggcatt
     6781 cgcaggagat catcgctata ttgtggatta tctggctgaa gaagtgctgc ggcgccaaac
     6841 ggaggaaatt cgcggctttc tattacagac atccattctt gagcgtttga atggttcgct
     6901 ttgtgacgcc gtgaccgccc aactcggtag taaatcaaaa ttggagcaat tgcaacgcgg
     6961 caatttattc ctcattcccc tcgatgacag gcgggagtgg taccgctatc accatctttt
     7021 tgcggatgtg cttcgtatgc atctggcggc ggaacaacca gaggcggttc ctgttctgca
     7081 ttgccgtgca agtgaatggt ttgagcaaaa caatatgacc gcggatgcga tccgccatgc
     7141 attggtcgga gaagattttg agcgggcggc ggggctaata gaaaaagttc tgcctatcat
     7201 gcgccaaagt aggcaggaac ctacattgct aaagtggtta aaagcgctac ccattaaatt
     7261 atttcagcac cgccccgttt tgaatgttca ctatattggc atgttattgc agaatggcca
     7321 gattgatggc gttgagtcac ggctgcggga cgttgagcaa tggctggctg ctcctgaaga
     7381 tattcgacga ggatccattt atgtggatga agttgaattt caacgcttac ccatttcggt
     7441 tgccatgtat cattccgcaa tggctctggc acaaggtgat gtggtcaacg caatgaaata
     7501 tgcaagcaaa gtgcttgaac tctcccgcga ggatgacgat tttccccgtg gcgcagcatc
     7561 ttccctgatg gggcttgcat cgtggacaaa cggcaacctc gaaatcgcat acgaaatgtt
     7621 tagcaaaggc atggcgcatt tgcagaaagt ggaatacgtc tccgatgtga tcggaggttc
     7681 cgttacgctc gctgatatac gcatcacgca gggtcgcctg cgtgatgcga tgagcattta
     7741 cgagcgcggg ctacaactgg cgaccaagtc agggattcca accttgcgcg gcgcggcaga
     7801 catgtacgtt ggcatggcgg aactccaccg tgaacacaat gatttgcagg ctgcaatgca
     7861 actgctcgag aaaagcaggg aacttggcga gttcaatggg ttgcccaaaa atccatatcg
     7921 attgcgtgtg gcaatggctg gactggaaga agcccaaggg aatttgaaca atgcgctcaa
     7981 tctgctcgat gaagcggaac ctcgatttgt ggctgacttc tcaccaaatg tgcggccgat
     8041 tcaggcattg aaagtgcggg tctggatcaa gcaaggcgag ttggagaagg ctctcgcttg
     8101 ggcgcatgaa cggaagttgt cgattaaaga agaacccagt tacttgcgag agtttgagca
     8161 aatcatgttt gcaagaacat tgttattgca aggcgacgat acagacagtt tgctgattga
     8221 atacttggaa cgccttctga aagcagcgga ggcaggcggc agaaatggga gtgtcatcga
     8281 aatcctgatc ttgcaagcgc tcgcctatca aatgcaagag gatattcctg ttgccttatc
     8341 atcgcttgaa cgcgccctca cactggctga gccagaaggg tacatccgca tgttcttaga
     8401 cgaaggtgca aacatggaga tgcttctccg tgaggctgcc gcgcacaaaa tcacgccaaa
     8461 ctacacgggc aaactgctgg cagcgtttga ggcggagcga agaggcttgg ttgtagaaac
     8521 gcctccttct gccgtaccag tttcagggtc cctgatcgag gcgcttagtc agcgcgagtt
     8581 ggatatcctg cgactgttca agaccgagtt atctggtccc gagatcgcac aggaacttgt
     8641 catcgccttg agtacggtgc ggacacatac aaaaagtatt tacaacaaac tcaatgtcaa
     8701 tagccgtcgg gcggctgtga agcgggcaat tgaacttggc ttgatctaaa acgaacaact
     8761 gaaatatcca aacaccccgc caactatctc tggcggggtg ttcaattctc cccacccgaa
     8821 atccccacat gtggggaaga catctcccca gatcgatctg tatattgtat tcaatcgaac
     8881 tcaacaggcg gcaacgccgg atgtcaatct cagacactta tcttattggt acaaaggaga
     8941 aaaaatgaaa gtgacaaata cgaaactcat ccgttgggca ggtttatctg ccgtcatagc
     9001 agggattatt ttcacggcta ttcagccgac ccatccatct atcaatacca gcgcattcat
     9061 catcatcact tccctcaaga cctccatctc gatctttggt ctgctcggta ttacggggct
     9121 ctacgcaagg caagtggaag aaaccggctg gaagggtctg gctggctatc ttttgctaac
     9181 catctattat gcagttcaga tgtgcatttc attcatcgaa cctactgtcc tgccgctgtt
     9241 gacgaccaca gcgccaacgt ttgtagaaag catgttgcag ttatccagcg ggactggcgt
     9301 ccccatgaac cttggcagcc tcacaaacgt gtattcgctc gtatccatac tttatgtcct
     9361 cggctccatg ctctttggca tggctatgtt tcgcgttcgc atcctgccgc gcggggcggc
     9421 tgccctgttt gccgtatcag gtcctttggc gggcaccatg tttaccctgc ttccctatca
     9481 tctcgtgcag ttgacatcga taccgattgg ggccgccatg gtctggctgg gatacgccct
     9541 cttttccgaa cgacgagaga aaaagtccgc atccttgctt gatcagggaa tcgtctcaca
     9601 ggtagcctga aaaccaataa tttttcaaag gagtttagaa atgaaagcga ttatccaaaa
     9661 taacagtcaa atcaaagcgc ctgcaagaaa aactcaaacg ccctggtggg tgcctgctgg
     9721 gctggtctta ctcagcgtca tcccgatcat ctttggcacg ctccgactca atcagttgat
     9781 caatggtgcg gagatcactc caggcaacgc acgcttcttt gcatcacctg cgcctgtggt
     9841 cattcacatt attagcgccg tggtctatgc cctgctcggc gcgttccaat ttatgagtcg
     9901 cctttggacg cgcggaatca aatggcatcg ctgggttggg cgattcctcg tcccagttgg
     9961 attattagtc gggctctctg gactctggat gactctcttc tatgactacc cggagggtgc
    10021 cagcaaacta ctgtacggac ttcgcctctt tttcggctcg ggcatggtcc tatccatcat
    10081 catcggattc atctcgatca gacggcgcga catcaatcag caccaagcct ggatgacacg
    10141 tgcctacgcc atcggaatgg gtgcagccac acaagtgctg accggcatcg tcggagcctt
    10201 gacccttggc gaagtcaatg agtttgaaaa tgcgctgttg atgggcgcag gctggataat
    10261 aaaccttgtg atcgctgagt tatctattcg caaaagtcag acccgtcaaa cccgcactgc
    10321 atagatttta gtatggatat ccactttatt aattttcaaa ataaggagat aaaaaatgaa
    10381 agcaattgta gcaacccaat acggcggtcc ggaagttctt caactcaaag aggtccagaa
    10441 gccgacaccg aaagacaatg agattctggt caaggtccat gccaccaccg tcaccgccgc
    10501 agattttcga atgcgaagtt tcaccgtccc tgtcgcagta tggatccccg cccgccttgc
    10561 actaggcatc acaaaaccca gacaaccgat cttcggctct gaactcgcgg gcgtagtgga
    10621 agcggtcggc aaagatgtga cccgcttcaa agtcggtgac caaatcttcg cctccaccct
    10681 gactgagaat tttggcggct atgccgaata caaatccctg cccgagaagg cgatgatggc
    10741 gatcaaacca accaacttga cctatgaaga agcagccgcc cttcccattg gcgcaaccac
    10801 tgccttgcgc ctgctccgca aaggaaacat ccagcgcgga caaaaggttc tgatctacgg
    10861 cgcgtcagga agtgtcggca catacgccgt gcaactggca aaatatttcg gcgcagaagt
    10921 gactgcggtg agcagcacat ccaatctgga gttggtgaaa tctctgggtg ctgaccacgt
    10981 gatggactac acaaaaggag acctcacatc cggagcagaa cgctatgatg tcatctttga
    11041 tacggtcgcc aaatttccga agtcacaata ttcgaatatc ctcgcagaga atggaagttt
    11101 tgtgtcaatg gcaaaactgg actccaaaga aagcatggac aacctggtct tcatcaaaga
    11161 cttgatcgag aagggtgaga tcaaagccgt gatcgatcgt tgctatccgc tggaagacat
    11221 ggtcgaagca catcgctatg tcgacacagg acgaaaaaaa gggaatgtcg tcattacggt
    11281 gccataatgc caaacggaca gaatttggag aacgacccag atcgaccaaa gatctttcgg
    11341 atcaggatcc aaggtcatct gggtcagcaa tggacaaact ggtttgacgg gttgaccatc
    11401 acattggaag aagatggtaa cacgcttcta gctgggcttg taaccgatca gtctgcgctg
    11461 cacggcgtct tgaaaaaaat ccgtgacctg ggaatgccgt tgttgtcggt acatgctatc
    11521 gaccccgatg agcaaacagg taactcatga acgatttatt tctctctcca gtcagcacaa
    11581 tgatcgttgg gctcggtgca accctcactt ttgatctctg ggggttattc ctcaaatatg
    11641 tcttcaagat cgctccatcc gatttctgtc tggtcgggcg ttgggttcta tacatgccaa
    11701 aaggaatctt taaacacaga aatatcagcg ctaccccgag gaaaagcacg gaatgtgtag
    11761 tcggatggtt tacacattac ctgacgggca ttttatttgc cattgctttt gttgctcaga
    11821 cgggaaatgg ctggcttcaa catcccaccc cgacccctgc cttgaccttt ggagttgtca
    11881 ccgtttcgat acccttcttc atcatgcaac ctgcatttgg gttaggggtc gccgcatcaa
    11941 aaactgcgaa gccgatgcag gcaagggtcc gcagtttggt aaatcatctt gtttttgggc
    12001 tgggtctctt catcttcgga ttgttggcaa gttggttgat ttagataata gcgacatcca
    12061 tcgcttcgct tctttacaat cgggtccatc cagtatgcac ttatcaacat acgaatacaa
    12121 attttcctgc caccatgcgg acaatgatct cgaaacaaaa ccgcctatcc acgtggatgg
    12181 gcggttttgt tttaaagatc tgttaccttc cgtacttcct tctcagatct tccgctgctg
    12241 cattcaactc tcctgcattt ctctggatct tcttgatcgc tgaaacaaca tcgtccacgc
    12301 ctttcgtacc cgcacggaaa gccgattcgt ccagccacac ggcttcgtgc tcgcaggcac
    12361 gggttgcttc aggcagttcc atggatttga aatcgaaata ctgcgggaag gcattcggta
    12421 ctgccagtct ggatttctgc ggctggaaga ggtcgtactt gtgcatggcg ttataaccgg
    12481 tccagcaatc cacgccttcg gcgtccaacg cgccgcacag caggtcatgc tccacaccaa
    12541 attcctgcgg atcgatcgca aagatatagc gatagaacga acgcgtggtg tggcgttcgt
    12601 cacgcttcaa cacacgcaca ccgcgaattt cactcaaggc ttcatccatg tatgccgcca
    12661 tctcttcgcg ctgcctggct tgttcgggga agcgctcgat gcctgtcaat gccatcgccg
    12721 cctgcaactc gctgattcgg aagttaccgc cctggacaga gagtccgttt gggtcttcgc
    12781 ccccaccgcc caacgcatgt ggacgcccac agtcgatgac cgaagcggca agcgcggcat
    12841 attcggcgga tttgcacaaa agaatcccgc cttcaccgga tgtgagcgtc ttcgatgatt
    12901 gcaatgagaa cgagccgaag tgaccgatcg tccccgcccc catgccgcgc cactttgcgc
    12961 cgtgagcgtg agcacagtct tcgatcacga ttagatcatg tttttccgcc agcgccatga
    13021 tggcatccat atctgccatg ttcgagccaa gatggacagg gatgatggct ttggtcttgg
    13081 gtgtgatggc tttctcagcc gccttcgcgt cgagacaata cgtgttcggg tcaacatcca
    13141 caatgacggg gatcgcgccc gctcccatcg gcgcggaggc ggtcgcctgg aaggtgtagg
    13201 caggcacgat gacttcgtca ccccaaccaa tgcccgcagc acgtaatgcc acttccatcg
    13261 tgatcgttcc gttcg
//
LOCUS       DAOQJE010000003        11985 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_2666_length_11985_cov_5.998910, whole genome shotgun sequence.
ACCESSION   DAOQJE010000003 DAOQJE010000000
VERSION     DAOQJE010000003.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 11985)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11985)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11985
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2666_length_11985_cov_5.998910"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            204..428
                     /locus_tag="PLT08_00120"
     CDS             204..428
                     /locus_tag="PLT08_00120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE02891.1"
                     /translation="MSEIDKRNVLDNEVFTYKINKEKKVFIYWRGKQVLILRGSEAQK
                     FTSKIIGLDDKGVQLVLAKLTGNFKRGNER"
     gene            510..2819
                     /locus_tag="PLT08_00125"
     CDS             510..2819
                     /locus_tag="PLT08_00125"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNE02892.1"
                     /translation="MPGREDIFQKAMNEGHSAAWDQEWSKAVAAYRRALEESPDNPKA
                     LSSLGQALHQLNNVEEALQIYMRAVKVSPDDPVSMEKVAQLSERVGDLKTATEAAIRA
                     GDLFLKQRDTDKALENWVHVTSINPEHAIAHSRLAQVHERLGHGQQAAMEYLAIASIL
                     QRAGNPEKTQEMVNKAQSLVPNSAEVKQAQTLLRTGQLLPKPIRAKGGTGPIRMAQVK
                     QLQEPAKKAASGLDPIAEARQKALTQLAEVLFDLSDDSSPVAQDRKGISAIMRGTGSL
                     SAQSEQLKVVLHLGQAIDAQSKGNDTLAAEEMEAALNAGFSSSALSFSIGYLRFKNER
                     YESAQRALQNSVKHEDYGLASRLLLGDIISRKGDFRDAATQYLEALKFADAATVPLEQ
                     GDDIRQQYEPLIESYQQQTDEAVLRKVCDNIRGLLMRADWRDQLHKTRDQMPKQDGMA
                     APLADMILQVQSSSVLESMNRINQLARMGSLRSAMDEAYDAIQHAPTYLPLHIIMGDL
                     LVQEGHTTDAIAKFSVVAHSYSVRGEVMQASKLLRRIIQISPMDLGARNRLIDQLVAR
                     GQVDDAIQEYLELAAIYYRLAELDMARKTYTHALRIVQQGNASRDWNTHILQRMADID
                     MQRLDWKQALRVYEQIRTLMPDDDASRKQLIELNMRMGQQDRAMSELESYITHLESQN
                     KQELAIAFLEELARDHEDQPLIKRTLAALLHRSGRIGEAIPILDQLGEALLEKGDKQG
                     AMEVINQIVLMNPPNVQDYRVLLSQMQNS"
     gene            2961..3995
                     /locus_tag="PLT08_00130"
     CDS             2961..3995
                     /locus_tag="PLT08_00130"
                     /inference="COORDINATES: protein motif:HMM:NF014665.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="COX15/CtaA family protein"
                     /protein_id="HNE02893.1"
                     /translation="MPKTQNRAVMTWLYVFAGVVAFLVVFGGFTRLTRSGLSIVEWNP
                     INGTVPPMAQHQWEAEFEKYKATPEYQLINTDMTLQEYKYIFYVEWFHRLVARMAGLF
                     YALPVFYFLIRKTIPLKEFGIYFWMGVLFIGQAVMGWLMVYSGLVDRPAVSHFRLTAH
                     LLLALTLFSLAIWTATGHRLGFHHYEKKAKWSLPTKISVAALVVLAIQISYGGMTAGL
                     KAGHVSDTWPLMFGKLIPPNLFNSFINILETPQTIVFIHRWFAWLGVILVPVVFYIIK
                     KQNYPADIQKGLLWLLGVVALQITLGVLTILSYVNIVIALLHQANAILLLGLAVYFIH
                     RFRAVDEAKA"
     gene            complement(4273..5304)
                     /locus_tag="PLT08_00135"
     CDS             complement(4273..5304)
                     /locus_tag="PLT08_00135"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNE02894.1"
                     /translation="MISELQEAKRMSVKIDEPTETLLAIHNLHVWYELRRFGFSVVGH
                     VKAVNNVSFELGKGETVAVVGESGCGKSSLMKTILGLNVPTGGELLFEGKRLTALTEA
                     ELRSYRFEIGYVQQDPYGALPPFMNVEQILEEPLIISGVSSKDERRERIHKILGEVKL
                     HPVEDFLHKYPHMLSGGQQQRVVIARAMVLEPKMIVADEPVSMLDASVRVEILKLMHA
                     LQEQHHLSVIYITHDLSTVKYFSARIFVMYAGSLIEKAETRQLLKNPLHPYTHALLAA
                     TSDPNAANAEVYKAVPPGEPPSLADPPKGCRFHPRCAKAMTGVCDVKEPPDFEPEPNH
                     FVACWLYEA"
     gene            complement(5321..5899)
                     /locus_tag="PLT08_00140"
     CDS             complement(5321..5899)
                     /locus_tag="PLT08_00140"
                     /inference="COORDINATES: protein motif:HMM:NF024273.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HNE02895.1"
                     /translation="MPSLALYDVLYSFKDIHKVVLEFASELNEEQLRWRPRGYSTSIG
                     FHLWHLARESDFLKAIILERTPQLGSDFGEPKEIWAVEKLAERWRFPTDLNATVGTGL
                     SDEIAATLPIPPKDELVDYLQKSYEALETFIALLDERYPTFEGVEEDLQRKLKNIRLN
                     LLVFLSHDCRHLGMMECLKGLQTGFGSATEKR"
     gene            complement(5917..6876)
                     /locus_tag="PLT08_00145"
     CDS             complement(5917..6876)
                     /locus_tag="PLT08_00145"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNE02896.1"
                     /translation="MTELTPLLEVKDLKLHFRTQKGVVQAVDGVDLDLGFHRAVVILG
                     ESGCGKTSLSKAMLRLLPRNVAKYSGELVINGKDVMPFTDEEYRQNVRWVSMSLVPQA
                     AMNALNPVITVGEQVSEPAMLHLGLNKDEALGMAYKMFQHVGIPADFISRYPFELSGG
                     MRQRVALAMALVTSPNLIILDEPTSALDLLTQANIMNVLKRIKHELGTSYILITHDIA
                     TSSELADEVAIMYAGQIVETGDAKDFFPAPLHPYSQMLMASVPRLRSESDPMFITGQP
                     PSLMNPPTGCRFAARCPFRFEKCDQEPPVFQKGNRKVKCWLHQ"
     gene            complement(6890..8227)
                     /locus_tag="PLT08_00150"
     CDS             complement(6890..8227)
                     /locus_tag="PLT08_00150"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNE02897.1"
                     /translation="MNNIVASFKKLLQYPSALVGIFVILVMVAVSIYAMVTIPYSEAV
                     RLWRGGEEVWYQNPKFAPPAWFNWFSSKKYSESFAVSTADGEMEKTSVPGNDGVTTYT
                     MTYEFDFKYDIYPQDMIVYVNALFDQKQPFISMEWITPDGRTIRVSNFATAAKSTYRF
                     SQDDKLAAKLRAEDPIPALFSDPDTGEVLKGPYKLVITGTTFEPNSNIDVELVVHGQV
                     YGLAGTDHARRDLTLPLLWGAPVALAFGLIAAIGTSVLTMVIAAVGTWYGGWVDQLVQ
                     RITEVNLVLPFFAIMIMIGTFYSRSIWTIISATIVLNIFTGAILGYRAIFLQVKESMY
                     IEAAKAYGATNWRIIFHYLIPRMIPLLIPGLVQAVPAFVFLEASLAVIGLGDPVLPTW
                     GKIIQDAQANGALYKGYYYWVLEPAVLLIITGLAFAFLGFVLDRIFNPRLRDV"
     gene            complement(8231..>8547)
                     /locus_tag="PLT08_00155"
     CDS             complement(8231..>8547)
                     /locus_tag="PLT08_00155"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNE02898.1"
                     /translation="YVDMARAKGLHAKDIERRYILRPTLPNIITNFSLLIIALWTGAL
                     FTETVFLWPGLGRTLYQAVGLYDTPIIVGSTIIYAYLLAMTVFLLDFIYAAVDPRVRV
                     GN"
     assembly_gap    8548..8647
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<8648..9507)
                     /locus_tag="PLT08_00160"
     CDS             complement(<8648..9507)
                     /locus_tag="PLT08_00160"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNE02899.1"
                     /translation="MSVVAPAASEQTAPKRKTALHALSRMLRYIAMRVLTLFTTVVIG
                     VYLTILIANMGGYVDQIIRGQIRDTITQSVANNPTLRNMDPETRRKLIEEQITLEEKR
                     RGLDTPIAIRNFRHLTQALQLDLGRAFNITSDSGSKQVRLILLERLPATLLLMGTSQL
                     FLFFSSIYIALNLSRNYGNIWDKLVIALSPTSAIPSWFYGIFLILIFAAGFKVLPFGG
                     MVSSPPPLNRFDYIISVLQHLVLPGGALVISSFFLSVYNYRTFFLIYSSEDYVDMARA
                     KGLHAKDIERR"
     gene            complement(9577..>11985)
                     /locus_tag="PLT08_00165"
     CDS             complement(9577..>11985)
                     /locus_tag="PLT08_00165"
                     /inference="COORDINATES: protein motif:HMM:NF012706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HNE02900.1"
                     /translation="GGWLDEIDFSVVSSDSAVSQLQAGAIDFYSFNLSSNTLQDIKDA
                     GLNYTQSYGGNYGISLNPAVFDDTTVLNPFSNRKIREAVNWLIDRNYINQEIYGGGSL
                     PKLLPITTQLVEYTNLIDTARALETKYAYNLERAREQVAAEMEAMGATMSSDGKWQFN
                     GEPVTLIFLIRSDGDGTRKPMGDYVANQLEEVGFTVDRQYKTSSEAFPIWLGSVASEG
                     QWHLYTAGYLPSGLTRDERANIQQYYLPTSIQASDPFISNVADPELQKLGDDLAQGNF
                     TSKEERDAMLARALDLALQDSLFVWVVDQLVYAPYNSNVSVTYDLAAGYEAAFMGNYN
                     LRFVDQEGGTMKVGTNDLFTQPWNTMAGSNWVWDSNIMRATSMGVASSGSASSGLMGD
                     PYTGLAWPQRIESASLEYQTGLPITDNKLGWVDVQAVDEIPVPEDAWVDWDATTQTFI
                     TVGQKYPDGTTAKVKSTVVYPADLFETVKWHDGSPISVADFVMSFVIFLDRGKPESAV
                     YDEAAALSVDAFLPSFKGWRITSTAPLTIEYYSDAYSADAELNVAPLWPGSPSGLSGE
                     NSWQILAISNLAEAAGELAYSSDKADANSIEQMSWVGGPSLEILKKYLDQAQTESYVP
                     YEATLGQFLTPEEIALRYENFSKWYADHGHFWVGTGPYYLDQVFTTEKSLVLKSFADF
                     PDLADRWSSFSDPKRARVILDGPGQVTAGQEATFDVTVNFGDEAYPTADIKQVKYILY
                     GTTGDVLEVGNAEAVGEGQFQVALSADATSKLETGSAKLEVAVVPIPVAIPAFTSFDF
                     VVVK"
BASE COUNT         3284 a         3015 c         2765 g         2821 t
ORIGIN      
        1 aaaaaataca gcaggtatat aaaggattgg tatgagtccg aaaaattcaa ccgactggct
       61 tttattctat attgtgacag gtcttatttt attttttata atatgtataa gcatattcat
      121 aatgctcttt cttgatggag ccttcctttg ggcatcctaa tattctagtt tgccatatag
      181 aatacaaaag atctttagac attatgagcg aaatagataa acgaaacgta ttggataatg
      241 aggtctttac ctataaaatc aacaaagaga aaaaggtgtt tatttactgg cgggggaaac
      301 aagtcctgat tttacgggga agcgaagccc aaaaatttac gagcaagatc atcgggctag
      361 acgataaagg agtgcaactg gtgctggcaa agctgacggg gaacttcaaa cgcggcaacg
      421 aacgctagcg cacgccccta accaataagg ttataattgc atgattgtta ggacgaccca
      481 ccctattttg aaagtaaaat aacaacacta tgcccggacg agaagatatc ttccaaaaag
      541 ccatgaacga gggacactcc gctgcctggg atcaggaatg gagcaaagcg gttgccgcct
      601 atcgccgcgc cctcgaagaa tcacccgata accccaaagc gctcagcagt ctcgggcagg
      661 cactccatca gctcaacaac gtggaagaag ccttgcagat ctacatgcgt gccgtcaagg
      721 tctcgccaga tgacccagtc tccatggaaa aagtggcgca actctctgaa cgcgttggcg
      781 acctgaaaac cgccaccgaa gccgccatcc gcgcaggtga cctcttcctc aaacaacgcg
      841 ataccgacaa agccctcgaa aactgggtac atgtcaccag cattaacccc gaacatgcca
      901 tagcccattc acgccttgca caagtccatg aacgtttggg gcatggtcaa caagccgcca
      961 tggaatatct tgccattgcc agcattttac aacgcgcggg caatcccgaa aaaacgcaag
     1021 agatggtgaa caaagcccaa tcgctggtac ccaacagcgc cgaagtgaaa caagcccaaa
     1081 cgttattaag aaccggtcaa ctcttgccca agccaattcg cgccaaaggc ggcacgggac
     1141 ccatccgcat ggcacaagtc aagcaattgc aggaaccggc gaagaaagcc gcctcagggc
     1201 tggacccgat cgcggaagca cgtcagaaag cgttgactca gctggcagaa gtcctcttcg
     1261 atctctccga cgacagcagc cccgtggcgc aagaccgcaa aggcatctct gccatcatgc
     1321 gcggaaccgg ctccctttcg gcgcaatcgg aacaactcaa agtggtcttg catttgggtc
     1381 aagccattga tgcgcaatcg aaaggcaacg acacgcttgc cgccgaagaa atggaagccg
     1441 cgctcaatgc tggctttagc agttcagctc tttcattcag catcggctat ttacgcttca
     1501 aaaatgaacg ctacgaaagc gcccaacgcg ctttgcaaaa ttcggttaag catgaagatt
     1561 atggtttagc ctcgcgcctg ttactaggcg atatcatttc gcgcaaaggc gacttccgcg
     1621 atgcggcaac gcaatatctg gaagctttga agtttgcaga tgcggctacc gttccactag
     1681 aacaaggcga tgacattcgc cagcaatacg agccgttgat cgagtcgtac caacagcaaa
     1741 cagacgaagc cgttttgcgc aaagtctgcg ataacattcg cgggctgtta atgcgtgcag
     1801 attggcgcga ccagcttcac aagacccgcg accaaatgcc caagcaagat ggcatggctg
     1861 cccctctggc agatatgatc ttgcaggtgc agtccagctc ggtgctcgag tcgatgaacc
     1921 gcatcaacca gttggcacgc atgggcagcc tgcgctctgc catggacgaa gcctatgacg
     1981 ccattcaaca cgcccccacc tacctaccgc ttcacatcat catgggcgat ttgctcgtac
     2041 aagaaggtca caccacagat gcgattgcca agtttagcgt ggtggcacat tcatacagtg
     2101 tacgcggcga agtgatgcaa gccagcaagt tattacgccg tatcatccaa atctcgccca
     2161 tggacctcgg cgcgcgcaat cgtctgatcg atcagcttgt agcccgtggt caggttgatg
     2221 atgctattca agagtatctt gaacttgccg ccatctacta ccgcctcgct gaacttgata
     2281 tggcacgcaa gacatacact cacgcattac gcattgtgca acaaggcaat gccagccgcg
     2341 attggaatac ccacatcctt cagcgtatgg cagatatcga tatgcagcgc ttggactgga
     2401 agcaggcttt gcgcgtctat gaacagatcc gcaccctcat gcccgatgac gatgcctctc
     2461 gcaaacaact gatcgaactc aatatgcgca tggggcaaca agaccgcgcc atgagcgagc
     2521 tggaaagtta catcactcac cttgaaagcc aaaacaagca agaacttgcc atcgccttcc
     2581 ttgaagagtt ggcacgtgac catgaagacc aaccgttgat caaacgcacg ctcgcagcgt
     2641 tgcttcaccg cagcggtcgc atcggagaag cgatacccat tctcgaccaa ttaggcgaag
     2701 ccttgcttga aaaaggggat aaacaaggcg ccatggaagt catcaaccag attgtgctga
     2761 tgaacccacc caacgtgcaa gattacaggg tgctgctgag ccaaatgcaa aatagttagt
     2821 caaaagcgtg gagttgctct ccacgctttt ttatacgctt ctcccgggtg acaaatcaca
     2881 gcaactttcg tgacaaaagt tctaacaatc tcctaagtcc tgaatgctat aatgccgcca
     2941 attcaaattg gagtaatcat atgccaaaaa cccaaaaccg cgccgtgatg acctggctat
     3001 acgtctttgc cggagtcgtg gcgtttcttg ttgtcttcgg tggttttacc cgcctcactc
     3061 gctctggtct ttccatcgta gaatggaatc ccatcaacgg caccgtgcct cccatggcac
     3121 agcaccaatg ggaagccgaa tttgagaaat acaaggcgac cccagaatat caactcatca
     3181 atacagacat gaccctgcag gaatataaat acatcttcta tgtagagtgg tttcatcgtc
     3241 tggttgcgcg catggcaggt ctgttctatg ccttacctgt tttctacttc ctcattcgca
     3301 aaaccattcc cctcaaagaa tttggcattt acttctggat gggcgttctc ttcatcggtc
     3361 aagctgtgat gggctggctg atggtctaca gcggattggt tgaccgcccc gctgtcagtc
     3421 acttccgcct cactgctcac ctcttgcttg ctcttaccct cttcagcctt gccatctgga
     3481 cggcgaccgg tcaccgactt gggttccatc actacgagaa aaaagccaaa tggtctttac
     3541 ctacgaagat ctctgtcgcc gcacttgtgg tgcttgccat tcagatcagc tatggtggca
     3601 tgacagccgg gctcaaagct gggcacgtct cagacacttg gcccctcatg ttcgggaaac
     3661 tcattccgcc caacctattt aattctttca tcaatattct tgagactccg caaaccattg
     3721 tctttattca ccgctggttc gcttggttgg gagtcatttt ggtgccagta gttttctaca
     3781 tcatcaagaa acagaattac ccagccgata ttcaaaaggg tctgctctgg ctactcggag
     3841 tggttgcctt acagatcacg cttggtgttc taaccattct ttcatacgtc aacatcgtca
     3901 ttgccttgtt gcatcaagcc aatgcaattc tgcttttagg gctggctgtc tacttcatcc
     3961 accgcttccg cgccgtcgat gaagccaaag cataaatact ttttgtaaaa atgcctcgcc
     4021 gtaaatggcg aggcattttt tattccgttt gccgccagtc ttctatctgt ggtggtctct
     4081 ttttgatagc cgtaacagac caacgcgtta acccaaacat gccaagcgcc aagccaacgg
     4141 tggatgagcc agaggtcaag aaaacgagaa aaaacgttgg ttcgaccact tttataatca
     4201 tcagaaacgg gagcacaaca cccaacagaa gcaaaagcaa gcccaagaac atgtagaagc
     4261 ggttttttat catcatgcct catataacca acaagccaca aaatgattcg gctcgggttc
     4321 aaaatctggt ggttctttca catcacaaac acctgtcatc gctttagcac aacgcggatg
     4381 aaagcgacat cccttgggcg ggtctgccaa actgggcggc tcgccgggcg gaacggcttt
     4441 atacacctca gcattggcag cattggggtc cgaggtagcg gcaagcaagg catgagtata
     4501 ggggtgcaat ggattcttga gcaactggcg cgtctctgct ttttcaatga ggctgcctgc
     4561 atacatcaca aaaatacgcg ctgaaaaata cttcactgta gaaaggtcat gagtaatgta
     4621 aatcactgaa aggtgatgtt gctcttgcaa agcatgcatg agcttcaaga tttccacgcg
     4681 caccgaagca tctaacatgg agacgggttc atctgccaca atcatctttg gttccaacac
     4741 catggctcgc gcaatcacca cgcgttgctg ctgcccgccg ctcaacatat gtggatactt
     4801 gtgcaggaaa tcttcgacag ggtgcagctt tacttctccc aatatcttat gaatacgctc
     4861 acgccgttcg tccttacttg aaactccgct gatgatgagt ggctcttcca agatctgctc
     4921 aacattcata aatggaggca aagcgccgta ggggtcttgc tgcacatatc caatttcaaa
     4981 gcgatagctg cgcaattcgg cttctgtcaa tgcggtgaga cgtttccctt caaaaagaag
     5041 ctcaccgccc gtgggcacat ttagccctag aatggtcttc atcagcgatg acttaccgca
     5101 tccactctcc ccgactactg ctacggtttc ccctttaccc aactcaaagc tgacgttatt
     5161 tacggctttg acatgcccaa ccacgctaaa gccaaagcgg cgaagctcat accaaacatg
     5221 taaattgtga atcgcaagca acgtctctgt cggttcatct attttcacag acatgcgttt
     5281 tgcctcttgt aattctgaga tcatagttgt ttatcgaatt tcatcgcttc tcagtagcag
     5341 aaccaaaccc cgtctgcaaa cccttcaaac actccatcat tccaagatga cgacaatcgt
     5401 gagaaaggaa aacaagcaag ttcaagcgaa tatttttcaa ttttcgttga aggtcttctt
     5461 ctacgccttc aaaggttggg taacgttcgt ctaacaaggc gataaatgtc tctaatgctt
     5521 cataggactt ctgcagataa tctaccaatt cgtctttagg cggtatgggc aaagtcgctg
     5581 caatttcatc acttaacccg gttccaactg tagcattgag gtcggtagga aaacgccagc
     5641 gttctgccaa tttctcaaca gcccaaatct ctttgggctc gccaaagtca ctgccgagtt
     5701 gcggggtacg ttctaaaatg atggctttca aaaaatcaga ttcgcgggct aaatgccaaa
     5761 gatgaaaccc aatagacgtg ctatatcctc gcggacgcca gcgtagttgt tcttcgttca
     5821 gttcagatgc aaactcaagc acgaccttat gaatatcttt gaaagaataa agcacgtcat
     5881 acaatgcaag ggacggcatg gatgaaactc cttggtttat tggtgcagcc agcactttac
     5941 tttacggttt cctttttgga aaaccggcgg ctcttgatcg catttctcaa aacggaacgg
     6001 acagcgcgcc gcaaagcgac accccgttgg cgggttcatc aaactgggtg gctgccctgt
     6061 aataaacatc gggtctgatt cgctgcgtaa gcgtggaacg ctcgccatta acatttgcga
     6121 ataaggatgc agtggtgcag ggaagaaatc tttggcatcg cccgtttcca cgatctgacc
     6181 ggcatacata attgctactt catctgctag ctcgctggag gtggcaatat cgtgtgtgat
     6241 gagaatgtac gaggtaccca attcatgctt aatacgtttg agcacattca taatattggc
     6301 ttgggtcagc aaatccaaag cagaagtggg ttcgtccaaa atgatcagat ttggcgaagt
     6361 aacgagtgcc attgccaaag caacccgttg ccgcataccg ccactcaact cgaatggata
     6421 gcgactaatg aagtcagcag ggatccccac atgctgaaac attttgtacg ccatgccaag
     6481 cgcctcatct ttattcaaac caagatgcaa catagctggc tctgaaacct gttctcccac
     6541 tgtaatgacc gggttcaacg cattcatagc cgcttgaggt acaagagaca tactcaccca
     6601 gcgcacattc tgccggtatt cttcatcggt aaacggcatc acgtcttttc cattaatgac
     6661 cagttcgccg ctatatttcg ccacatttct aggcagcaag cgcaacatgg cttttgaaag
     6721 cgatgtttta ccgcaccccg attcacccag aatgactaca gcgcggtgaa accccaagtc
     6781 gaggtctaca ccgtcaacgg cttgcacaac tcctttttgg gtacggaagt ggagcttcag
     6841 gtcttttact tcaagcaagg gtgtcaactc tgtcattgat acgtctcggt tacacgtcgc
     6901 gcaggcgcgg gttgaagatg cgatctaata caaagccgag gaaagcaaaa gccaacccag
     6961 tgataatcaa caaaacagca ggttccaaca cccagtagta atatcccttg tataaagctc
     7021 cattcgcctg agcatcttga ataatcttgc cccaagtcgg taggacgggg tctcctaaac
     7081 cgatgacggc aagagacgcc tcaaggaaca caaaggctgg cacggcttga accaaacctg
     7141 gaatcaacaa ggggatcatg cgtggaatga ggtaatgaaa aataatacgc caatttgttg
     7201 cgccgtatgc ttttgccgct tcaatgtaca ttgactcttt gacctgaaga aatatggcac
     7261 gatacccaag aatggcacct gtaaagatat tcaagacaat cgttgcactg ataatggtcc
     7321 aaatactacg ggaataaaat gtaccgatca tgatcatgat cgcaaaaaac ggcaacacca
     7381 aattgacttc ggtaattcgt tgcaccaact ggtcgaccca gccgccatac catgtgccaa
     7441 cagcggcaat gaccattgtt aagaccgaag tgccgatagc tgcaataaga ccaaacgcca
     7501 atgccacagg agcaccccac aaaagcggta gggtcaaatc acggcgggca tgatccgtcc
     7561 ctgccaagcc atagacctgt ccgtgaacca cgagttctac atctatattc gagttaggct
     7621 caaaagtggt gccagtaata acgagcttat agggtccctt taatacctca ccggtatctg
     7681 ggtcagagaa caaggctggg atggggtctt ctgcccggag tttggctgcc agtttatcgt
     7741 cttgtgagaa gcgataggta gacttggctg cggtagcaaa atttgaaaca cgaatcgttc
     7801 gcccatcagg tgtaatccac tccatcgaaa tgaacggctg tttctggtca aaaagggcat
     7861 tgacataaac gatcatatct tgagggtaga tatcgtactt aaaatcgaac tcgtatgtca
     7921 ttgtgtatgt ggtgacacca tcattcccgg gtacagatgt tttttccatc tcgccatcgg
     7981 cagtacttac tgcaaaagat tcagagtatt ttttgctgga gaaccagttg aaccatgcgg
     8041 ggggagcaaa cttcgggttt tgataccaaa cctcttcccc gccacgccac aagcgcacag
     8101 cttcagaata tggaatagtg accatggcat agatagaaac tgccaccatc accaaaatga
     8161 caaagatacc taccaaagca gatggatact gaagtagttt tttgaaggag gctacgatat
     8221 tgttcatggc ttaatttcca acccgcacac gggggtcaac tgcggcataa ataaaatcaa
     8281 gtagaaagac cgtcatagca agtaaatatg catagatgat ggtagagccc acgatgattg
     8341 gtgtatcgta taaaccaacc gcttgatata gggttctccc aagcccgggc cataagaaga
     8401 ccgtctctgt aaataaagct cccgtccaaa gtgcaataat gagcaatgag aagtttgtaa
     8461 taatgtttgg cagagttggg cgcaaaatat aacggcgctc aatgtctttg gcatgcaagc
     8521 ctttggcacg tgccatatca acataatnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     8581 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     8641 nnnnnnncgg cgctcaatgt ctttggcatg caagcctttg gcacgtgcca tatcaacata
     8701 atcttcgctg gaataaatca aaaagaaagt tcgatagttg taaacactaa gaaaaaatga
     8761 actaatgact agagcaccac caggtaagac aaggtgctgc agaacactga taatataatc
     8821 aaagcggttt aacggcggcg gcgaagagac catgccacca aacggaagga ctttaaagcc
     8881 cgccgcaaag atcagaatta agaaaatacc gtaaaaccaa gagggaattg ctgaggtggg
     8941 cgagagtgca attacaagct tgtcccatat gtttccataa tttctagaaa gattcagcgc
     9001 aatatagata ctagaaaaga aaaggaataa ttgcgatgtg cccattagta ataatgtagc
     9061 aggcagtcgt tccaacaaaa tgagccgaac ttgtttcgag ccgctgtcac tggtaatgtt
     9121 aaaggcacgc ccaagatcaa gttgaagtgc ttgcgttaag tggcgaaagt tacgtatggc
     9181 tatcggagta tccagtccgc gtcttttttc ttcaagtgtg atttgttctt cgattaactt
     9241 ccggcgtgtt tctgggtcca tgttgcgcag ggtgggatta ttggcaacag attgtgtgat
     9301 cgtatcgcgg atctgccccc gaatgatctg gtctacatac ccgcccatat ttgcgattaa
     9361 gatcgtaaga taaaccccga tcaccacagt agtaaataag gtcaacacac gcatggcgat
     9421 gtaacgcaac atacggctta gagcatgaag ggcggttttt ctctttggag cggtctgttc
     9481 agaagcagcg ggagcaacaa cggacataaa tacctcgcaa gggttcaggg tgaggcgaaa
     9541 tgcctcaccc tgaattattg gaatttctac atcaacttac tttacaacta cgaaatcaaa
     9601 ggaagtgaaa gcagggatag ccactgggat cggtacaact gccacttcga gtttggcgga
     9661 accggtttcc agtttggagg tcgcgtcggc actcaaggca acttggaact gtccctcgcc
     9721 cactgcttca gcatttccaa cttcaagaac atcgcctgtg gtgccataca aaatgtattt
     9781 cacctgcttg atatcggcag tcggatacgc ttcatcgcca aagttgacgg tcacatcgaa
     9841 ggtcgcttct tgccccgccg ttacctgacc cggtccatct aagatgacac gtgcccgttt
     9901 cgggtcgctg aaagaagacc aacggtcagc aagatcgggg aaatctgcaa agctcttgag
     9961 cacgagagac ttctctgtag taaagacctg atcaagatag tacggaccag ttcccaccca
    10021 gaaatgtcca tggtcggcat accatttgga gaagttttca tagcgaagag cgatttcttc
    10081 cggagtcagg aactgaccca aggtcgcttc atacggaaca tagctttcgg tctgagcctg
    10141 gtccaaatac ttcttgagga tctcaaggct tggtccgcct acccagctca tttgctcaat
    10201 gctattagca tctgccttat cggaagagta agccaattcg ccagcagctt ctgccagatt
    10261 ggagatcgcc aaaatttgcc agctattctc accagaaagc cctgacgggg aaccgggcca
    10321 caacggagca acattcaact cagcatcagc agagtacgca tcagagtagt attcaatggt
    10381 gagcggggcg gtagaggtga tacgccaacc tttgaaggaa ggcaggaaag catccacgga
    10441 aagagccgcc gcttcatcat agactgcact ttcaggctta ccgcgatcaa ggaaaatgac
    10501 gaacgacatg acaaaatctg cgacagagat tgggctgcca tcatgccatt tgacggtttc
    10561 aaacaaatcg gcagggtaca ccaccgtgct cttaaccttg gcagtggtgc catctgggta
    10621 tttttgaccc actgtaatga aggtctgagt cgttgcatcc caatcgaccc aagcatcttc
    10681 cggaaccgga atttcatcca cagcttgaac atctacccag ccaagtttgt tatcagtaat
    10741 gggtaaacca gtttgatatt caagagaggc gctttcaata cgttgcggcc aggcaagccc
    10801 ggtgtatggg tcacccatca agccagaaga ggcgctgccg gagcttgcca cacccatgct
    10861 ggtagcacgc atgatgttcg aatcccacac ccagttgctg cccgccattg tgttccaagg
    10921 ctgagtgaag aggtcattcg taccgacctt cattgtgcca ccttcttggt ccacaaaacg
    10981 aaggttgtaa ttccccataa atgccgcttc gtaaccagct gccaggtcat aggtcaccga
    11041 gacattgctg ttgtaaggag catagaccag ttgatctaca acccatacaa agagtgagtc
    11101 ttgaagggca aggtctaagg cgcgcgccaa catggcatca cgttcttctt tactagtgaa
    11161 gttaccttga gcaagatcat caccaagttt ctgaagttcg gggtccgcta cattggagat
    11221 gaacgggtcg ctcgcctgaa tactggtggg aagatagtat tgctgaatgt tcgcgcgctc
    11281 atcgcgggta agaccagagg gcaaataacc agcagtataa agatgccact gtccttcaga
    11341 agctacggag ccaagccaga ttgggaaggc ttcagaagat gtcttatatt gacggtctac
    11401 agtaaagccc acttcttcca actggttggc aacataatcg cccatgggtt tacgggtgcc
    11461 atcgccatcg gagcggataa ggaagatcaa ggtgacaggt tccccattaa actgccattt
    11521 gccatcagag ctcattgtgg cacccatggc ttccatttca gcggcaacct gttcgcgagc
    11581 tctctcaagg ttgtaggcat acttggtttc aagagcgcga gcggtatcga tcaaattggt
    11641 gtattcaacc aactgggttg tgataggcag aagttttggt aaggaaccgc cgccatagat
    11701 ttcctgattg atatagttac ggtcgatcaa ccagttcacc gcttcacgga tcttgcggtt
    11761 cgagaaggga ttcaagaccg ttgtgtcatc gaacacggca gggtttaaac tgatgccata
    11821 gttaccacca tacgattggg tatagttcaa gccggcatct ttaatatctt gcaaggtgtt
    11881 tgaagacagg ttaaacgaat agaagtcaat tgcaccggct tgcaattgag aaactgccga
    11941 atcagatgaa acgactgaaa aatcaatttc gtccaaccag ccgcc
//
LOCUS       DAOQJE010000004        11602 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_2800_length_11602_cov_4.521607, whole genome shotgun sequence.
ACCESSION   DAOQJE010000004 DAOQJE010000000
VERSION     DAOQJE010000004.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 11602)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11602)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11602
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2800_length_11602_cov_4.521607"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..765
                     /locus_tag="PLT08_00170"
     CDS             <1..765
                     /locus_tag="PLT08_00170"
                     /inference="COORDINATES: protein motif:HMM:NF012493.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-related protein"
                     /protein_id="HNE02901.1"
                     /translation="EGFNMQDASILVNYDLPWTVLQLAQRMGRVLRPWGAPRDVIIYN
                     FVPSTMDHKRIRHARNWEHRLQERSRQHRSLAQIPVMVHRESQQQGMTQELEMEKLGR
                     EMFLTSDDGADLDLEQVMEFLHTVEDLTTSTFYNDLAIISNQNEILKLPAGIRSAMNK
                     PGRKRLFLLLKRGKNRIDTIIADVHGKPMEESYRRDDVMRIIRCLPETAKATFESFPQ
                     EDEFDLWIERTRLNWTKQNGLDSGKIQVICALALVP"
     gene            865..1551
                     /locus_tag="PLT08_00175"
     CDS             865..1551
                     /locus_tag="PLT08_00175"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HNE02902.1"
                     /translation="MNDRIIREKMNTMKSKGTSTENKLGLFIESYYSQLLVGIRVLIW
                     KMGMASTPKEADDLSQEILSETILTAIEIENRYDKDKSMRAWLLSIAANKIRELRTKE
                     TRHGKRMGSVTETYINSSKRTTSLQKQYKDIEGITEDEMIDHLNSYNSLQSHARKRLH
                     LSFDELISFVDTNDQEILRLAFENNLKGKDLAATLRISSGAANVRLSRAISRLKQAYL
                     ASENSKRIKK"
     gene            1548..2114
                     /locus_tag="PLT08_00180"
     CDS             1548..2114
                     /locus_tag="PLT08_00180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE02903.1"
                     /translation="MNTNNLSDKREKTVQRYVSALDSGNLDDIILVLAEATEDPVLDQ
                     MIFDVNEAIEQEDNLTPIANDANLVLNLLQEHFANPETGNEKTQITIGDVAARMQADR
                     KIPSTDQEIHKHLLGINIPLPEILNIQAIHELANNLQLIASDKFWRVFRDTAIMLGIG
                     RGQAHMAAARKQSKNKSKENTHRDVKDK"
     gene            2120..2836
                     /locus_tag="PLT08_00185"
     CDS             2120..2836
                     /locus_tag="PLT08_00185"
                     /inference="COORDINATES: protein motif:HMM:NF017885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HNE02904.1"
                     /translation="MSNHYMPAQIKSIVNQLLKKTHSSYKENNIIPLYDIVCSHPIRI
                     AEIEDLSFHTAAQFLSSETGQSVPLPENERQELAGFLFIQRYRKTLYGCILVEKKDSI
                     VRRRFSVAHELGHYLLHFLPEFESQHASQDLVFAEGLTYSSNLTDDLPTGKLALIQSL
                     ENLSGFNFIENELVEKEANQFAAELLIPESRCKDMYRDFTKKYGVQRPVLIKRLATEF
                     LVSAEAMKWRLMALKLHPSL"
     gene            2847..3896
                     /locus_tag="PLT08_00190"
     CDS             2847..3896
                     /locus_tag="PLT08_00190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE02905.1"
                     /translation="MGLDFILNNGGRRSATDITLPIPYNYSSNRDNNLQNEVDLELEM
                     SDKAWQAINIVNTLNWRPIEWEYSPIRFVDGKDVGQTVAWVYSPDGYPVPIRLSQIGS
                     IVMNLENGEIRRDYSTVDRVVSMVVDPFSWEDIEGFARSLQANGIRLLPAYPLDPNAT
                     FEFEKLRQASQDRTREEMSVLEEAAIARNNNVPTVIDGRLDKHSGGFDRSYSPVFGVI
                     KTHYQNYLNRTGMQVLYDLKAGERTPFFRLTPNQRPSVITWYLRLSDAIGTTPTWGFI
                     RIEVSQDWFFMNQQNENFVNRLSRAIYEYRCKESSYRRAPVSIHPIVRAEESLGSQYQ
                     PDSQIIADFYRQSGI"
     gene            3920..5608
                     /locus_tag="PLT08_00195"
     CDS             3920..5608
                     /locus_tag="PLT08_00195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNE02906.1"
                     /translation="MKNNPQIIGRVASPPQLESTSEQFYFWVQRNQIVEKTQIVRVES
                     NVDGQCFQYYGVVEEVNRRSRKKNIEEEFDFADGDVNYESELKAEGVTYASVRILRTQ
                     PAILTPPIEYSKVFLAEQPDADFAYGFSEMSRALPIGQLYNGADRFAGLAQIDLFYLL
                     GENGGHMNVSGMAGAGTKSSFLLAMLKLLSHHAKNSKIKEPLYIVPIILNVKGEDLMW
                     INHPNKNFIEEKHLTDWNALNIPPEPFAGAEFFAPADKNNPSAPAIDGCNARVYSWAL
                     ADILERDLFFYLFSDDDFSSTMQALVYDVIAYLTETTDGKVKLKNNVPQDWQKLLDWL
                     RLQSGLKDADREISMHQTGTWRAVYRRLWDILAEGDGIFLHNKTHGNPLIVTRQQSSS
                     PIVVDIHSLPTSLQRFVVAAIMKQVTEARMGRNAVRGLRYVIVLDELNRFAPKNNSDP
                     ITKLLERVASELRSQGVILFGAQQLASDVSPKIIENSAIRVLGRTGSAELQDKVWQSW
                     DKSSRQQANVLGPNEKLITQPGFRQPMYVRVPFPSWAMRREDIAAKRLEELPEI"
     gene            5610..6776
                     /locus_tag="PLT08_00200"
     CDS             5610..6776
                     /locus_tag="PLT08_00200"
                     /inference="COORDINATES: protein motif:HMM:TIGR00619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease SbcCD subunit D"
                     /protein_id="HNE02907.1"
                     /translation="MRILHTSDLHLGSRLHGIDRTDELFSQFENICEIASQQKVDVLL
                     VAGDVFEKRGIALPELTKKLADILAPRIREGMHVILVPGNHDDREHFNMMNALLSLEQ
                     GYSEKVHIVKTKEILKIQNTQFVAIPYPLHEMLQPYMAEFSGSTERNVSLSSAFAGLI
                     RSVIDNVDPALPAILIAHIYVAGTTTPNNYELTYDNDIRLGKADLPILNNLKYIALGH
                     IHQFQKIDHPIPCYYCGSMDRLNWGEKDEDKYVNVVDVFDNGDAKVTSLPLGTTPFHF
                     IQTTASQLENLRSMYHDLNQAFVTLEITNDTGVDPATIYRIAKDICPRQISINIVGEN
                     EKVQKPKLSMQPRGYAETSQKYLEEFFKDDPDLPELQRLTSELLNEVENATTAN"
     gene            6757..9858
                     /locus_tag="PLT08_00205"
     CDS             6757..9858
                     /locus_tag="PLT08_00205"
                     /inference="COORDINATES: protein motif:HMM:NF024867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMC family ATPase"
                     /protein_id="HNE02908.1"
                     /translation="MLLQRISLLNFLSHRGISNDNSRITPVEIDLRVSPLWLIYGPNG
                     SGKSSIFDAITFALFKEHRGSGSANHGSGYLISDGENEAKIELEIVLNEKSYLIQREL
                     KRKKTSLSSADISGIVLEWDGKKWVAVKNTKNKVEEWVKKNLRMGSKTFTSAVLLQQG
                     EADAFLKAKPKDRKDRLLEILDLDFYKLLGEIAASKKNNARNEWRQLEQKLSMLRKVD
                     DVEIKSQNQVIAQISSDLSNANILVSNKKIELRDAEQVLKMKESIKLKEDAKKQVEKL
                     LKREEQIISNVKRYRELSSDVPMARNILEIKGRIKQENEEQKKISISLIAKDRSLTEI
                     SENIKKSQKDTLAAKKDHANKIQELESIRVERDTFKLKADQIVQIEELESLLAKTKAE
                     LTPYQDTLKQSKELDQEKQRYEEIKKFLPAIEDLYQYQEQASKINTEKHKCEIDLSEA
                     KKQLELITSQVQQERKDLLSANRAQEKFRLASEKIEARINQIKEKIKEKEEIHNKDEC
                     PVCGSPLNTDEVHLRTEAQIKKLKENLANLLKESKIVNASLRDASVDVSSKDSSIRKL
                     EGKKNKIETNKSVLESKLESAIKRESENKKLIKKQEVRVGDWVREIGNITSLQKELAQ
                     LEKNFKKWEKLEVARKEASKLQAVLAMRESDLSRLPKLTTVQRQKAISSYNRAVEEYI
                     SLHEETKLAEKISRDCQNALDEFQKRETSLLQEVAHLKDNLENSNKRKSNEEEQLVKK
                     LSSLPDRLKSIIDQIETKELGRLESEMTSLSGSEKEEAELRKARKEADQIEGSLAQLR
                     KQLQEIPKEHNRSVDVVRLEFEAASHDVEVLQEKLNQANEAIGKLRSAQEEYERNIKS
                     FEDAKTKLRHFEKLSSAFGRSGLQAQIVQDAQKKIKEAANTTLGYLSNGGWQIELSGD
                     DQELEIFAQDLNSPGLPMRRFEFLSGGEKFRVAISLAVAIGQSISGGRTVDTLIIDEG
                     FGSLDEVNRDNLVVELRRLSEEVLNGGRVVIVSHENDICEEFTHRKKIYKNAEGLVCI
                     END"
     gene            complement(10052..>11602)
                     /locus_tag="PLT08_00210"
     CDS             complement(10052..>11602)
                     /locus_tag="PLT08_00210"
                     /inference="COORDINATES: protein motif:HMM:NF012790.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HNE02909.1"
                     /translation="FLARSSVSMPTINFFQLVFILVAVGVGFVLLRPLLFGGRRIAAA
                     AAYPKIQAGEAVLIDVREPDEWRSGVAAPAQLLPLSDLQGSRSKWAPFLAANKDKELI
                     LYCASGIRSGKAALLLRKEGYKTGGNPVQFSIDKIWRIEKGTFKNEAIVLYGKKISDI
                     ENSLTISGEHVSQERTSPLIFNKIIQGNRVAWSEKLNNLAGMIGFFEPADFRQGADIM
                     PRTLIFHELLKSRNKKWTLGPINRQESEFKYLVADAKKHKHFSLPQCVISDQFVFDVM
                     MSNHLTPFDLSKPAKGFLPIRKNVDGIWQPLHPAELATSQVEAQAFNRIFSEIGMNVG
                     LEDFFQLLDSNRRKLTSQVIPENKWIVYMGAGGNYVCAAYSGPDTNLFEIRKLIIDQT
                     LYWSSVDTEDEAIYLTGLLNSDAVNEVIKEFQPRGQFGPRHVHKLPIGVTPKFNPNEA
                     GHVNVVETTRNLIVEWNALKTVDAEIASYLNPNKKLSQRRTYLRNKIKALQSYDNYAD
                     ACRSLYGI"
BASE COUNT         3867 a         2206 c         2413 g         3116 t
ORIGIN      
        1 gaaggtttca acatgcagga tgcatcgatt ctcgtaaatt acgatctgcc ttggacggtg
       61 ctgcaattgg cacaacgcat gggaagagtg ttacgcccgt ggggtgctcc gcgggatgtc
      121 atcatctaca attttgtgcc ttccacaatg gatcacaaac ggattcgcca tgcccgcaac
      181 tgggagcatc ggttacagga acgaagccgg cagcatcgtt ctctggctca gatcccggtc
      241 atggttcata gggagagtca acaacaaggc atgacccagg aattggaaat ggaaaagttg
      301 gggcgtgaaa tgttccttac atcggatgat ggcgcagatt tggaccttga acaggtcatg
      361 gaatttctac ataccgttga agatctgact acaagcactt tttataatga cttggcaata
      421 atttctaatc aaaacgagat attaaagttg ccagcaggta tccgtagtgc catgaacaag
      481 cctggtcgca aacgattatt cctattgttg aagcgtggga aaaataggat tgatactatc
      541 attgccgatg ttcatggcaa gccgatggag gaaagctacc gccgggatga tgtgatgcgg
      601 ataatccgtt gtctgcctga aaccgccaaa gcgacgtttg aaagttttcc tcaagaagat
      661 gaattcgatt tatggattga gcgcacaagg ctaaactgga caaaacaaaa tgggctagat
      721 tcagggaaaa tccaggtcat ttgcgcacta gcccttgtac catagggaaa ataattgact
      781 tcaaattgtt caataaatgc ctgtaataaa tcgccctact tacgccatat gtctattgta
      841 accataaatg gctgcaggag gcttatgaat gatcgaataa tcagggaaaa aatgaatacg
      901 atgaagtcca agggtacctc aacagagaac aaattgggtc tctttattga atcatattac
      961 agccagcttt tggttggtat tcgtgtgctc atttggaaaa tgggcatggc aagtactcca
     1021 aaggaagctg acgatttgtc acaagagatt ctgagtgaaa caattctgac agcgattgaa
     1081 attgaaaacc gttatgataa agataaatct atgagggcat ggttgttaag tattgctgcc
     1141 aacaaaataa gagaactaag aacaaaagaa accagacatg gtaaacgaat ggggtctgtc
     1201 acggaaacat acattaattc atctaaaaga acaacctcct tacaaaaaca atataaagac
     1261 attgagggca taacagaaga tgagatgatc gaccacctaa attcgtacaa tagcctccaa
     1321 tctcatgcaa ggaaacgact tcatttaagt ttcgatgaat taatttcatt tgttgacaca
     1381 aatgatcaag aaatactaag actggcattt gaaaacaact taaaaggtaa ggatttggca
     1441 gcaaccttga gaatttccag cggagcagca aatgtgcgcc taagccgagc tattagtcga
     1501 ttaaagcagg catatcttgc cagtgaaaat agcaagagga ttaagaaatg aatacaaata
     1561 atttatcaga taagcgtgaa aaaactgtac aacgctatgt gagtgctcta gatagcggaa
     1621 acttagatga cattattctt gttcttgctg aagctacaga agaccctgtt cttgatcaga
     1681 tgatatttga cgttaatgaa gcaatagaac aggaagataa tttaacccct attgcaaacg
     1741 acgctaattt agttctaaac cttttacaag agcatttcgc taatcctgaa acaggcaatg
     1801 aaaagacaca aatcactatt ggggatgtcg cagcaaggat gcaagcagat cgaaaaatac
     1861 cttctactga tcaggaaatc cataaacacc ttttagggat aaacatacct ctacctgaga
     1921 tattaaatat tcaagcaatt cacgaattag caaataatct gcaacttatt gctagtgaca
     1981 aattctggag agtattccgt gataccgcaa tcatgctagg tattggtcgc gggcaagccc
     2041 atatggcagc cgcccgaaaa caatcgaaaa ataaatctaa agaaaacact catcgagatg
     2101 tcaaggataa gtaatgaata tgtcaaacca ttacatgcca gcgcagataa aaagtattgt
     2161 taatcaatta ctgaaaaaaa ctcactcatc atataaagag aataacatta ttcctttata
     2221 tgacattgtc tgttctcatc caattcgcat tgctgaaatc gaagatttat ctttccacac
     2281 agctgcacaa ttccttagtt cggaaacagg tcaaagtgtc cctcttccag aaaatgaaag
     2341 acaagagtta gctggttttc tttttatcca aagatatcgc aaaacacttt atggatgtat
     2401 cttggtggaa aagaaagact cgatcgtacg tcgtcgtttc tctgtcgctc acgaacttgg
     2461 acactattta cttcacttcc tcccagaatt tgaatctcaa catgcctcac aagacttagt
     2521 tttcgctgaa ggtctcactt actctagtaa tttgaccgac gacctaccta ccgggaaatt
     2581 agcccttatt cagtctttag aaaatttatc aggattcaat tttatcgaaa atgaattggt
     2641 agaaaaagaa gcaaatcaat ttgctgccga gttgcttata ccagaatcaa gatgcaagga
     2701 catgtacaga gattttacta agaagtatgg tgttcagcgc ccagttctaa tcaagaggct
     2761 agcaacagaa tttctagtaa gtgctgaggc tatgaaatgg cgcttaatgg cacttaagtt
     2821 gcacccctct ctataaggag cactttatgg gattggattt tattctaaac aacggcggtc
     2881 gccgatcagc aacggatata acactaccaa taccttacaa ttattcctcc aatcgcgata
     2941 ataacttgca aaatgaggtg gatttagaat tggaaatgtc agataaggca tggcaagcaa
     3001 ttaatattgt gaacactcta aactggcgcc ctatagaatg ggaatactct cccatccgct
     3061 ttgttgacgg aaaagatgtt ggacaaacag ttgcatgggt gtattcacca gatggttatc
     3121 ccgtgccgat tcgtttgtct caaataggta gtattgtaat gaatctagag aatggagaaa
     3181 ttcgtagaga ttactccaca gttgatcgag ttgtttcgat ggtagtagat ccgttttcct
     3241 gggaggatat agaaggattt gcacgatctt tacaagctaa tggtattcgt cttttgccag
     3301 cctatccatt agacccaaat gcaacttttg agtttgaaaa acttcgtcaa gcctcccaag
     3361 atcgcacacg cgaggaaatg agcgtgttag aagaagcagc aattgctcgg aacaataatg
     3421 tacctacagt aattgatgga aggctggata aacattccgg cgggtttgat cgaagttatt
     3481 ctcctgtgtt cggcgttatc aaaactcatt atcaaaacta tcttaataga acaggaatgc
     3541 aggtattgta tgacctaaaa gcaggtgaaa gaacgccgtt tttcaggttg acaccaaatc
     3601 aacgtccctc tgtgattact tggtacctac ggctatctga tgctattgga acaacaccaa
     3661 cttggggttt tatccgtata gaggtttctc aagattggtt cttcatgaat caacaaaacg
     3721 aaaattttgt aaatcggtta tcccgtgcaa tttatgaata tcgttgtaaa gagtcgagtt
     3781 atcgtcgtgc cccagtttct attcatccca tagttcgcgc tgaagaaagt ctcggatcgc
     3841 agtatcaacc tgacagtcaa atcatagctg atttttatcg gcaatctggc atataagtgt
     3901 tcagataaaa ggagatttta tgaaaaacaa tccccaaatt attggacgtg tagcttcacc
     3961 gccacaatta gagtcaactt cagaacaatt ctatttttgg gttcaaagaa atcagattgt
     4021 cgagaaaact caaatcgtgc gtgtcgaaag caatgtcgat ggtcaatgtt ttcaatatta
     4081 tggagttgtc gaagaggtta atcgacgtag ccgcaagaaa aatattgaag aagaattcga
     4141 ttttgccgat ggtgatgtga attacgagtc agagttaaaa gcagaaggtg taacttatgc
     4201 gagtgttcga attctgagaa cacaacctgc catccttacg ccgcccatag aatacagcaa
     4261 ggtttttcta gcagagcaac cagatgccga ttttgcttat ggtttctctg agatgtcacg
     4321 tgctttaccg attggacaat tatataacgg cgctgatcga tttgctggac ttgctcagat
     4381 cgatttattc tatttactag gtgaaaatgg cgggcatatg aatgtatctg gtatggcagg
     4441 tgcaggtacg aaatccagtt ttttacttgc aatgttgaaa cttttaagtc atcatgcaaa
     4501 aaactcaaaa atcaaagaac cactttatat cgtgcccatc atcctaaatg taaaagggga
     4561 ggatttaatg tggatcaatc atcctaacaa gaattttatt gaagaaaaac acctaacaga
     4621 ctggaatgcg ctaaatattc cacctgagcc atttgcaggc gctgagtttt ttgcacccgc
     4681 tgacaaaaac aatcctagtg cgccggctat tgatggctgc aatgctcgtg tttactcatg
     4741 ggctcttgca gatattcttg aaagggactt attcttttac ttgttttctg atgatgattt
     4801 ttcatccacg atgcaagcgc tggtatatga cgttatcgct tacttaaccg aaacaactga
     4861 cggaaaagtt aaactaaaaa acaatgtacc acaagactgg cagaaacttt tagattggtt
     4921 gcgccttcaa tctgggctga aagatgcaga cagggaaatc tcaatgcacc agactggtac
     4981 ctggagagca gtatatcgac gactttggga tattcttgca gaaggagatg gcattttcct
     5041 tcacaacaaa actcatggaa atcctcttat tgttacgcgt caacaatctt caagtccaat
     5101 tgttgttgat attcacagtt tgcctacttc cttgcaaaga tttgttgtcg ctgcaatcat
     5161 gaaacaagta acagaagctc gtatgggacg aaatgctgtt agaggattaa gatatgtgat
     5221 tgtgcttgat gaattaaatc gttttgcacc aaaaaacaat tcagatccta ttacaaaatt
     5281 gctagaacgt gtcgcgtcgg agctacgcag ccaaggagta atcctttttg gagcgcaaca
     5341 acttgcttcg gatgtttcgc caaagattat tgagaattct gcaatacgcg tattaggacg
     5401 cactggatct gctgaacttc aagacaaagt atggcaatct tgggacaagt catcaaggca
     5461 acaagcaaat gtacttggtc ctaatgaaaa attgatcacc caacctggtt ttaggcaacc
     5521 aatgtatgtt cgagtcccat tcccttcatg ggctatgcga cgggaagaca tcgcagcgaa
     5581 acgattagaa gaactaccgg agatataaaa tgagaatcct gcatacttct gaccttcatc
     5641 ttggatcgag gcttcatgga atagatcgta ctgatgaatt gttttcacaa ttcgaaaaca
     5701 tttgcgaaat tgcaagtcaa caaaaagtag atgtactgct tgtggctggt gatgtctttg
     5761 aaaaacgtgg aatcgcatta ccagaattaa ctaagaaact tgcagacatt cttgctcctc
     5821 gaattcgaga agggatgcac gtcattttgg tgcctggcaa ccatgacgac agagaacact
     5881 tcaacatgat gaatgcgtta ctatctctag agcaaggata tagcgagaaa gttcacatag
     5941 ttaaaacaaa agagatactt aagattcaaa acactcaatt tgtggcaatt ccctatcctt
     6001 tacatgaaat gctacaaccc tatatggctg agttttctgg ttctacggaa cgaaatgtat
     6061 ccctgagttc ggcatttgca ggattaattc gatctgtaat tgataatgtt gaccctgcgt
     6121 tgcctgcaat tcttattgca catatctatg tggctggaac aacaacacct aataactatg
     6181 aacttaccta tgataacgat attcgtctag gtaaagccga tttgcccatt ttgaataatc
     6241 ttaaatatat tgctctagga catattcatc aatttcagaa aattgatcac ccaataccct
     6301 gctattattg tggcagtatg gatcgcctaa actggggcga gaaagatgaa gataaatatg
     6361 tcaacgtagt tgatgtcttt gataatggag atgccaaggt aacatccctg ccgcttggca
     6421 caacaccatt tcattttatt cagacaacag catctcaatt agaaaatttg agatcaatgt
     6481 atcatgatct caatcaggct tttgtgacgc ttgaaataac aaacgatact ggggtggatc
     6541 cagcaaccat ttaccgaatt gccaaagata tttgcccaag acaaataagc attaatatag
     6601 ttggagaaaa cgaaaaggta caaaaaccta aattgtcaat gcagccaaga ggttatgctg
     6661 agacatctca aaaatacttg gaagaattct tcaaagatga tccggatttg ccagaacttc
     6721 aaagattgac aagcgaatta ctcaatgagg tggaaaatgc tactacagcg aattagtctt
     6781 cttaatttcc ttagccatcg aggaatttct aacgacaact caagaattac ccctgtcgaa
     6841 atcgaccttc gcgtgtcacc attatggctc atttatggac ccaatgggtc tggtaaaagt
     6901 tccatttttg acgctatcac ttttgcgtta ttcaaagaac accgcggaag tggtagtgcc
     6961 aatcatggtt caggttattt gattagtgat ggagaaaatg aagcaaagat agaactagaa
     7021 attgtcttaa atgagaaatc gtatctaata caaagagaat taaagcgaaa gaaaacatca
     7081 ttatcatcgg ctgatatttc aggtattgtt cttgaatggg atggaaagaa atgggtggca
     7141 gttaaaaaca caaaaaataa agtcgaagaa tgggttaaga aaaatctacg aatgggttca
     7201 aaaacattca cttcagcagt attactccag caaggtgaag cggatgcatt tcttaaggcc
     7261 aaacccaaag acagaaaaga tcggctttta gagattttgg atttagattt ctacaaatta
     7321 ttaggagaga tcgctgcgag taaaaaaaac aacgccagaa acgagtggag acaattggag
     7381 caaaaactct ctatgttgag aaaagttgat gacgttgaaa ttaaatcgca aaaccaagtt
     7441 atcgcgcaaa tttctagtga cctttcaaac gcgaacatac tggtctctaa taaaaaaata
     7501 gaactaagag acgccgagca agtgttaaaa atgaaagaga gcatcaagtt aaaagaagat
     7561 gccaaaaaac aagttgagaa attacttaag agagaagagc aaataatttc taatgtaaag
     7621 cgttacaggg aattatcgag cgatgttcct atggctcgaa atattcttga aattaaagga
     7681 cgcatcaaac aggaaaatga agaacaaaag aaaatctcca tctccttaat tgccaaggat
     7741 cgttctttga cagaaatttc ggaaaacatc aagaaatctc aaaaagacac acttgcggca
     7801 aaaaaagatc acgccaataa aattcaagaa cttgaaagca tcagagtgga gcgcgacaca
     7861 tttaaactaa aggctgatca aattgttcaa attgaagaac tggaaagttt attagctaaa
     7921 acaaaagcag agctgacgcc atatcaagat accttaaagc aatcgaaaga gcttgaccaa
     7981 gaaaaacaac gctacgaaga aatcaagaaa ttcctgcctg ccattgaaga tctttatcaa
     8041 taccaagagc aggcatccaa aataaataca gaaaagcaca aatgcgagat tgatctttct
     8101 gaagccaaaa aacagcttga attaattaca tctcaagttc aacaagaaag gaaagattta
     8161 ttgagtgcca acagagccca agaaaaattc cggttagctt cagaaaaaat agaagcacgc
     8221 atcaatcaaa taaaagaaaa aataaaagaa aaggaagaaa tccataacaa ggatgagtgt
     8281 cctgtctgtg gaagccctct aaatacagac gaggtacacc taaggactga agcccaaatc
     8341 aaaaaattga aagaaaattt agccaacctt ctaaaagaga gcaaaatagt aaatgcatct
     8401 ttgcgtgatg caagtgttga tgtgtcttca aaagacagca gcatccggaa gttagaagga
     8461 aaaaagaaca aaatagaaac caataaatct gtcttggaaa gtaaactgga aagcgctatc
     8521 aaacgcgaaa gcgaaaataa aaagttaatc aaaaagcagg aggtgagagt tggcgattgg
     8581 gtcagagaga ttggcaatat cactagttta caaaaagaat tagctcagct tgaaaaaaac
     8641 tttaaaaaat gggaaaaatt agaagttgcg cgaaaagaag ctagtaaatt acaggcagtc
     8701 cttgccatgc gcgaatctga cctttcaaga ttacccaagt tgacaacagt tcaacgccaa
     8761 aaggctattt cttcttacaa tcgtgctgtc gaagaatata tttctctaca tgaagaaacc
     8821 aaacttgcag agaaaatcag tcgcgattgt cagaatgcct tggatgaatt tcaaaaaagg
     8881 gagactagtc ttttacaaga agtagcgcat cttaaggata atttagaaaa ttcaaacaag
     8941 cgaaagtcta atgaagaaga acaacttgtc aaaaaattaa gttcgctgcc tgatagatta
     9001 aagtcaataa tagatcaaat tgaaacaaaa gaattgggca ggcttgaaag cgaaatgact
     9061 tccttgtctg gctcagaaaa agaagaagct gaactaagga aagcgcgaaa ggaagcagat
     9121 caaattgaag gtagtttggc acagttacgt aaacaattac aggaaattcc aaaagagcac
     9181 aaccgctcag ttgatgtagt tcgtttagaa tttgaagcag catctcatga tgtagaggtt
     9241 ttacaggaaa aactcaatca agccaatgaa gcaatcggaa aattgagatc ggcacaagaa
     9301 gaatatgaac gaaatataaa gtcatttgaa gacgcaaaaa cgaagttaag gcacttcgaa
     9361 aaattgagct ctgcattcgg gcgctcaggt ttacaggcac aaattgttca agatgcgcag
     9421 aaaaaaataa aagaggctgc taatacaact cttggctatt taagtaatgg tggttggcaa
     9481 attgagcttt caggtgatga ccaagaatta gaaatctttg ctcaagatct taatagtcct
     9541 ggattgccaa tgcgccgatt cgagtttcta agtggaggcg agaaatttcg agttgcgatc
     9601 agcctagcgg ttgcaattgg gcagtctatc tctggcgggc gaacagtaga tactctaatt
     9661 atcgatgaag gttttgggtc tttggatgag gtaaatcgcg ataatctcgt tgtcgaactg
     9721 agaaggttaa gtgaggaagt gcttaatggc ggcagggttg tgatagtctc ccacgaaaat
     9781 gatatttgtg aagagttcac tcatcgtaaa aaaatctata aaaatgcaga agggcttgtc
     9841 tgtattgaaa acgattaaga caaacaaatt aaaatatcca accaggagtt cgttgatgtt
     9901 caatatatca tcctgatagt tcgggcagtt tatgcgcgca aaacgaaaaa aacgatattt
     9961 tccttcaaca gccagccttg atactaacca gatcatcatg ctggctgttt ggcgaaatcc
    10021 aattcaggat taacttcttc aggctttatt tttatatacc atataagctt ctacatgcat
    10081 cagcataatt atcataactt tgcaatgcct tgattttatt tcttaagtat gtacgccttt
    10141 gcgacaattt tttatttgga ttcagatagc ttgcaatttc tgcatcaaca gtctttaatg
    10201 cattccattc aactatcagg ttacgcgttg tttctactac attaacgtgc cccgcttcat
    10261 ttggattgaa ttttggagtt accccaattg gtaatttgtg aacatgccga gggccaaatt
    10321 gcccacgagg ttgaaactcc ttaataactt cattaactgc atcactattc aataaaccag
    10381 taagatatat cgcttcatcc tcagtgtcaa cagaactcca gtacagcgtt tgatcaatga
    10441 tgagctttct tatttcaaac aaatttgtgt ctggacctga ataggctgcg cacacataat
    10501 ttccgcctgc tcccatataa acaatccact tattttcggg gattacttga cttgttaatt
    10561 tccttctatt gctatcaagt aattggaaga aatcctccaa acctacattc ataccaattt
    10621 ctgaaaaaat tcggttaaat gcttgtgctt ctacttgact agtagctagt tcagctgggt
    10681 gtaatggttg ccaaatcccg tccacatttt ttcgaatggg aagaaatcct tttgcaggtt
    10741 ttgataaatc aaaaggagtt aagtgatttg acatcattac atcaaaaaca aattgatcag
    10801 agatcacaca ttgtggaagt gaaaaatgtt tatgcttctt tgcatctgca actaaatatt
    10861 tgaactcact ttcttgacgg ttaattggac ctagtgtcca ctttttattt cgtgatttta
    10921 ataattcatg aaaaataaga gtgcgtggca ttatatctgc accctgtctg aaatctgctg
    10981 gctcaaaaaa acctatcatc ccagctaaat tattcaactt ctcgctccag gcgaccctgt
    11041 taccttggat tatcttattg aatattaacg gactagttct ttcttgtgaa acatgctctc
    11101 cagaaatggt caaagaattt tcaatatcag aaatcttttt cccgtacaaa acaatggcct
    11161 cgtttttaaa tgttcctttt tcaatcctcc agattttatc aatgctaaat tgaacagggt
    11221 ttccaccggt cttgtaacct tcttttcgca gcaggagcgc cgccttgccg gagcggatgc
    11281 cggaggcgca gtagaggatg agttccttgt ccttgttggc ggcgaggaac ggcgcccatt
    11341 tgctgcgcga tccctggagg tcgctgagtg gcagaagctg cgcgggcgcg gcgacgccgc
    11401 tcctccattc gtcgggctcg cggacgtcga tcagcaccgc ctcgccggct tggatcttcg
    11461 ggtacgcggc ggcggcggcg atgcgacgtc cgccgaagag cagcggacgc aggagcacga
    11521 agccgacgcc gacagcgacg aggatgaaga cgagctggaa gaagttgatt gtgggcatgg
    11581 agacggagga cctagcgagg aa
//
LOCUS       DAOQJE010000005        11113 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_2991_length_11113_cov_6.220112, whole genome shotgun sequence.
ACCESSION   DAOQJE010000005 DAOQJE010000000
VERSION     DAOQJE010000005.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 11113)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11113)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11113
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2991_length_11113_cov_6.220112"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..200
                     /locus_tag="PLT08_00215"
     CDS             <1..200
                     /locus_tag="PLT08_00215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE02910.1"
                     /translation="HYDFSDLPLFSPIAPQLGLKGPLNGKRVTEIVNSYLIDFFEMTL
                     KNKPSELFSNVSPFSEVVPIP"
     gene            395..781
                     /locus_tag="PLT08_00220"
     CDS             395..781
                     /locus_tag="PLT08_00220"
                     /inference="COORDINATES: protein motif:HMM:NF008551.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HNE02911.1"
                     /translation="MNIKHIHHVAIICSDYERSKKFYTEVLGFTVKLEHYRAERQSYK
                     LDLCINDHYQVELFSFPNSAERANYPEARGLRHLAFAVEDIDAAISDLRGKGTEPEAV
                     RIDELTGKRYTFFKDPDGLPLELYEE"
     gene            947..1849
                     /locus_tag="PLT08_00225"
     CDS             947..1849
                     /locus_tag="PLT08_00225"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF019474.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HNE02912.1"
                     /translation="MKLPFLATSLLLSILLAACNRPLQQNSVPENSTPTTLNLPTTIV
                     YKTVANVDTDLLSLDIHSPANAQNAPIVMWIHGGGYTIGDKANQMNNKISLFNEQGWV
                     LVSVNYRLSTPEKGAAKFPDHFLDVAAAVAWVHENIQGYGGDPNRIALVGHSAGADIV
                     SNVVVNPNYLREYGLELSNIICAASLDTEGFDKTKADENEQKMWQNALSNNQNYLTET
                     SATLLIHPNIGIPPMLGVTRGNERRQQIATEFITTLQEAGIEATLIDATSLTHEEVNN
                     QIGAAGDTVMTAPLMSFLTECFER"
     gene            2177..2689
                     /locus_tag="PLT08_00230"
     CDS             2177..2689
                     /locus_tag="PLT08_00230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE02913.1"
                     /translation="MSKSDLSPEHILLRNGALLIIPPLVITFGLWGALPVAYSPDVFW
                     KDIPEWLGVFENVFRIFVFSIPGMLYFGKAEKGQPLGWYLYFGGLLIYLASYGAQIVH
                     PGSLWSQSIIGFTAPAWSTVFWFAGIGLVCARSWLPIPWCRAIYLVIASCFLIFHIWH
                     TGLVYFSITQ"
     gene            3013..3435
                     /locus_tag="PLT08_00235"
     CDS             3013..3435
                     /locus_tag="PLT08_00235"
                     /inference="COORDINATES: protein motif:HMM:NF016001.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family deazaflavin-dependent
                     oxidoreductase"
                     /protein_id="HNE02914.1"
                     /translation="MKDLFIKLFMFINAQIIRLSRGKVGSKLGTQTILILHTIGRKSG
                     EERAIPIAYFKYENKYLIVASNWGKDQQASWYLNLKKNPSATLEVQGKVISTNAREAE
                     GAEYAHLWSFVTKLHPPYSDYQKLTARRIPIMIFDPIG"
     gene            3466..4218
                     /locus_tag="PLT08_00240"
     CDS             3466..4218
                     /locus_tag="PLT08_00240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE02915.1"
                     /translation="MLIKKYTLLVLPFLFILSGCGLNRASVPTPLPTYTQPSSPTSPP
                     ATETALPTSTAEFVATATNIATATPPPVCHVGETIESATNGSLPSYIDIVKVSTKLSG
                     SKFSATFTMNWLPDQIMIDRNILGYGAAEIAWGVAIDVDNDPNTGSSIPLTSSGYGYD
                     YVLQAANFKEGEEVQGGLQALFSDKVNVWEVFADGSNSVLYQGKINVDTATASITLSG
                     DIKGIKKDSYLQFFAIYFPTASQQLTDEICQR"
     gene            4215..4565
                     /locus_tag="PLT08_00245"
     CDS             4215..4565
                     /locus_tag="PLT08_00245"
                     /inference="COORDINATES: protein motif:HMM:NF019498.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HNE02916.1"
                     /translation="MNREPVSTSNAEHYIWGQVCDGWHLLRQDALSVIQERVPAGRTE
                     VMHYHTSARQFFYVLEGEGQIAFEDQTVTLKKGEGLEIQPQVWHRFENNSQTDVHFLV
                     ISSPKAQGDRVNVE"
     gene            4587..5060
                     /locus_tag="PLT08_00250"
     CDS             4587..5060
                     /locus_tag="PLT08_00250"
                     /inference="COORDINATES: protein motif:HMM:NF019632.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClbS/DfsB family four-helix bundle protein"
                     /protein_id="HNE02917.1"
                     /translation="MSNVKEELLQMYKKSQADITSQLDEIDKNRKIYPLWTVREIIAH
                     LSGWDDSITGMLNALLKGDVPPTPAARGVDVYNAETVSTREGLSYDHVIREYYKTREK
                     LFALISQANDDILSKPSILPWGEEGSVAMLVKFFSEHEAEHAEDIKKFIETATQK"
     gene            5122..8307
                     /locus_tag="PLT08_00255"
     CDS             5122..8307
                     /locus_tag="PLT08_00255"
                     /inference="COORDINATES: protein motif:HMM:NF015194.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005471 - ATP:ADP antiporter activity
                     [Evidence IEA];
                     GO_process: GO:0006862 - nucleotide transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Npt1/Npt2 family nucleotide transporter"
                     /protein_id="HNE02918.1"
                     /translation="MKSRILALLNIRRGEEKNVFLMLTQYFFMGAAMLFVQSASLALF
                     FTAWDATAMPYIYLGIAVIVSTITAIFLKISERTSLARFLILSVLFVLFGSIALRIGL
                     AFTSSKWLLLALPIWSQTLVNITVTAFWTLAGNIFDVRQGKRIFGLMNAGSWLSYVVL
                     GPFTTPLVNLIGTENLYLVIAACLLISFFLQQAVIHANPRTETNQETTDQQSQQTSIA
                     QLFRIRYIALIFALITIWRIAYFILDNIFYDRAALQYPTADSMAGFIGGFFAITGLLG
                     FITDMFLTGRIISRFGLRAGLLATPTLTLLFTAALAATGTLKPEMLPAIFWLAVAGKF
                     TNEGLGFSLDQTADNLLYQPIHERIRARAQTVTEGIIQPLAIGLAGGMLLLFNTALKF
                     NAIQLAYIYIAFAIIWIIICIALIRAYPVALTEALHKRRFGDKGILLTDASSIQIVRM
                     ALKSQHASEVLYALDLLEKGESDTFIDDLETQIRSPMPEVRKDVIQRIERLKITKLLR
                     DIQIQLRTETDPNVREIASRALIVLGADSSTALSLLAQEDAATQRGAMIGALRILRES
                     KDSNAYERLLELTKSKQVQDRVYAAQLMAEAANPILSNVLLALLSDEDLQAKKAALRA
                     AAKFQHPDLYSKIIPALGLPLTRSLAFNALVEGGNHAIPSIIEALKDISLHRRIHLRL
                     IRACSHIKSEAAIHVLEGLIHHSNVSVRSRALTALHDCRYKPEGESAFGVSEQVTAES
                     RRAAWLLGVMHDLGKSTKTEVLVRAASHDLDGTIDRLFHLFEFIYEPQAIQRAQHAIQ
                     RKDANRKSYAIEVLDTTLSKPHKAAFIPLLEDFAPKETLEKMGSEYKQTFLPPAGRVM
                     DILTNPLAKQNPWLVATAIEFADSMGISLDSEVTDSLSSSNESLLARMVLQKRKEKTM
                     LSTVERVIVLKSLNMFEKTPDEALAELAELVQEMVVQPGDEIIREGETGDSLYIIVDG
                     KVEVVDDNRILNQLGARAVFGELSMLDSSPRTATIRAIEETSLLRLDQTSFYEIMSDY
                     VEVAMGTIQLLTRNLRARTGDVMELSRMLGQ"
     gene            8321..8713
                     /locus_tag="PLT08_00260"
     CDS             8321..8713
                     /locus_tag="PLT08_00260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE02919.1"
                     /translation="METKPALDSKLVEEFVGNSHGNFARVKELLEQEPALVNATWDWG
                     GGDFETALGAAAHMGNKQIANYLLEHGARLDIFAAAMLDKLDVVKAALEAYPEAVNTP
                     GPHGIPLYVHAEAGDAKSVLEYLNSLEG"
     gene            8779..9531
                     /locus_tag="PLT08_00265"
     CDS             8779..9531
                     /locus_tag="PLT08_00265"
                     /inference="COORDINATES: protein motif:HMM:NF024777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE02920.1"
                     /translation="MKKRNQVVIGLIAAIIMLACACPVSGLPFGSEEQPTPGLPILPV
                     ATIVDAPATEAPVQPAANVLLSDDFSSPSAEMETYSGDGGSAGTENGVYVVRSTADLW
                     QWGKSNSQFDNAIFEADITMIAGPTNNNAGFGIICRLSERDDTSIDGYMFAISGDGYY
                     TIRHIVSSNMTALVDWTESSVINQGNASNHLRATCNGSDLRLEVNGQEVASAIAPADG
                     PTNGTMAFAAISFETDQPYAEGHFDNLTVSQP"
     gene            complement(9579..10547)
                     /locus_tag="PLT08_00270"
     CDS             complement(9579..10547)
                     /locus_tag="PLT08_00270"
                     /inference="COORDINATES: protein motif:HMM:NF019121.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE02921.1"
                     /translation="MRVCVLSDEYIEDFNPAPYLKDYDWKMVTLTAPVEEKIKALAES
                     GEFDVFLNVCEGYEFEDEEDEELGYEASEVVEALEALNLPFTGADSRCFDPTREEMQT
                     IADANGVGFAKGYRVHSVEEAEHLVKNLRYPIMVKHPKSYGSTGMFKDSRADTLEQVL
                     TQVKRVCSEFGAARMEEFIIGKEFNVLVVDNPADMDNPFVYPPAELIFPDGEEFWHTD
                     IKWDYNIPFDFKEVTEPELKAKLQNEGKRMFQAMGISGYGRCDIRMNEKGELFILEIN
                     PNPAIMLPPKEYGPADYMILYDNDGYKGFFDRIFEAAFLRQKMRAK"
     gene            complement(10682..>11113)
                     /locus_tag="PLT08_00275"
     CDS             complement(10682..>11113)
                     /locus_tag="PLT08_00275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HNE02922.1"
                     /translation="QTVVLGRGFNYATAFEWALKLKELTYIIAEPYSSADFAHGPIAM
                     VESGYPVLAVAARGKVFDSMLEMLKRLRSDIAAELVVISNDRKALLLAQVPLQIPVDT
                     PEWLSPLVSILPAQLFAYHLTLAKGYNTEQPRSIRKVTETK"
BASE COUNT         3118 a         2599 c         2379 g         3017 t
ORIGIN      
        1 ggcattatga ttttagcgac ctgccattgt tctcgccaat cgcgccacaa cttggactga
       61 agggtccgct aaacggcaaa cgtgtgactg agatcgtcaa ttcgtatcta atcgatttct
      121 ttgagatgac cttgaaaaac aagccttctg agctttttag caatgtcagt ccattttcag
      181 aagtagttcc tattccataa tcgtgagagg tagtatgaac ttcaataaac gcagagtttc
      241 gggatatttg ttgatcttcg gcatggcttt tttggtaatc ggtattacta ctgataatac
      301 aattttctct tggatatcgg ttgggtttat agtgatctcc ttgtttttgg gcggacgttg
      361 gatgcgccca agaagacgcc cataaaggtt taagatgaat ataaagcaca ttcatcacgt
      421 ggctattatt tgctcggact atgaaaggtc caaaaagttc tacacagagg tattaggctt
      481 caccgtgaaa ttagaacatt accgagcaga gcggcagtct tataaattag acttgtgcat
      541 taacgaccac tatcaggtcg aattgttttc ctttcctaat agtgccgagc gggcaaacta
      601 cccagaagct cgagggttac gtcacctagc atttgcggta gaagatattg acgctgctat
      661 ctctgacctg cgaggaaaag gaactgaacc cgaagcagtg cggattgatg aactgaccgg
      721 aaagcgctat acctttttca aagaccccga tggtctgcca ctggagcttt acgaagagtg
      781 attttttccg actggacgga tgagcaaagt ctgtgcaccg ggtaggtaaa gtcagccgtt
      841 gggtggctat cgccaaagaa caaaaacaaa aggctattaa caaaatttac aactcggtaa
      901 cattcacaaa atatatttaa gtaacaatga aagtaaggag acttttatga aactgccctt
      961 tttagcaacc agccttctac tttctatttt actagccgcc tgcaatcgtc cacttcaaca
     1021 aaatagtgtc cccgaaaatt ccacgccaac aacccttaat ttaccaacca ctattgtcta
     1081 caaaactgtt gcaaatgttg atactgactt actaagcctc gacattcact ctcctgcaaa
     1141 tgcccaaaac gcacccattg ttatgtggat tcacggtggt ggatacacca tcggcgacaa
     1201 agccaatcaa atgaacaata agatttccct gttcaatgaa caaggctggg ttttagtcag
     1261 cgtcaattac cgattaagca caccagagaa aggcgcagcg aaattccccg accattttct
     1321 agatgtggct gcggctgtcg catgggttca tgagaatatt caaggctacg gtggcgaccc
     1381 gaaccgcatc gctttagtcg gtcactcagc aggcgcagac attgtatcca atgtagtagt
     1441 taatccaaat tacttaagag aatacggatt ggaactgtcc aacatcattt gcgccgcttc
     1501 gcttgacaca gaaggttttg ataagacaaa ggcagatgaa aacgaacaga agatgtggca
     1561 aaatgcgcta agtaataacc aaaattatct aaccgaaaca tctgccactc tgcttatcca
     1621 tccgaatatt ggtattccac ctatgctggg ggtaacacgc ggaaacgaac gtcgccaaca
     1681 aatagcaact gaatttatca caactttaca agaggctggt attgaagcca cacttataga
     1741 cgccacatct cttacacacg aagaagttaa caaccaaatc ggcgcagcag gcgatacggt
     1801 gatgactgca ccattaatga gttttttaac agaatgtttt gaaagataat tttttattgg
     1861 gcgtatatct cgcagtatag ttaatttaaa gcaaagctcg agccaataaa gcatcccgaa
     1921 aaacatcggg taatgcacct aatctcttct gtcctttgga tatcttttcc taaagaggag
     1981 atgccaatat atgctttttc tagctaagcg ttttttctgc tatcaagcag agtccgtttc
     2041 taattagacg aatgagataa atctgcgtgt caggaaagtt cacctcgtcc ttgtacgcat
     2101 ttcagttatg catcctactt gctctattaa cagacttata atccctacat aatattaact
     2161 acatcgagag ttaaacatgt ccaaatctga tttatcccca gaacacatat tacttagaaa
     2221 tggtgctctc ttaattattc ctccactagt cattaccttc ggtctttggg gcgcattacc
     2281 tgtagcatac agtccagatg tattctggaa agatatacct gaatggttgg gggtattcga
     2341 aaatgtgttt cggattttcg tttttagcat cccaggaatg ctgtactttg gaaaagcaga
     2401 aaaggggcaa ccgttaggct ggtatttata tttcggtgga ttattgatct accttgcaag
     2461 ctatggggcg caaatagttc acccgggtag tttatggagt caaagcatca ttggctttac
     2521 agccccggct tggtccactg tattttggtt tgctggcatc ggtttagtat gtgcccgcag
     2581 ttggctccca attccatggt gtcgtgcgat ttatttagta attgcatcct gctttttgat
     2641 cttccatatc tggcacacgg ggttggtcta cttcagcata actcaataag ttgttttgca
     2701 aaacatctaa taaatatatt gaagaactct tttgaacaca aaaacttaag aaccagtaga
     2761 attttattta tttttggagg agttgctttt attgttgctt atcttttcag caaacaagta
     2821 gcttttatag ggataggctc ttctttaatt gtcaaatcta ggtcaacgcc tcaaaattaa
     2881 tcccttgttt atggttagac ataatgttta cttaattaaa acttagacct taattcaaat
     2941 tcaaacgccc atcagtaaga ttttttccat tctccctatc tctttcatat catcacaccc
     3001 aacgaggacg atatgaaaga tcttttcatc aagcttttta tgttcatcaa cgctcaaata
     3061 atccgcctga gccgtggaaa agtgggcagc aaattaggaa cacaaactat ccttattttg
     3121 cataccattg ggagaaaatc tggcgaagaa cgcgccatcc caattgctta ttttaagtat
     3181 gaaaacaaat acctgatcgt agcatccaat tggggtaaag accaacaagc aagttggtac
     3241 ctcaacctga aaaagaatcc atcggcaacc cttgaagtgc agggaaaagt catttccacg
     3301 aatgcacgtg aagcagaagg tgcagaatat gcgcacttat ggagttttgt gacgaaactc
     3361 catccgcctt atagcgatta tcagaagttg acagcccggc gtatccccat catgatattt
     3421 gacccaatcg gataaaatta ccgaaaaaga ttggtttaag ttttcatgtt aatcaaaaaa
     3481 tatacgcttc ttgtcctccc ttttctgttc attctttctg gctgtggtct taatcgtgcg
     3541 tcggttccta ctcccttgcc aacatatacc caacccagtt caccaacaag cccgcctgca
     3601 acagagaccg ccctacccac gtcaaccgct gaattcgtag ccactgcaac aaatattgct
     3661 acggcaaccc cacctccagt ctgccatgtc ggtgagacga ttgaaagcgc aaccaacggc
     3721 tctctgccaa gctatattga tattgtaaaa gtatcaacca aattaagcgg aagcaagttt
     3781 tcagccacat ttacgatgaa ttggctgcca gaccagatca tgatcgaccg taacattctt
     3841 ggctatggag cggcagagat tgcttggggg gttgccatcg acgtagataa tgaccctaac
     3901 accggctcct ccatcccgct caccagttct gggtatggct atgactatgt ccttcaagca
     3961 gctaacttca aagagggcga agaagtgcaa ggcggactgc aagccttatt cagtgacaaa
     4021 gtaaatgtat gggaagtatt tgcagatggc agtaattctg tattatacca aggcaagatc
     4081 aacgtggata cagccaccgc ctccattacg ctaagcggag atatcaaggg catcaaaaaa
     4141 gattcctacc tgcaattctt tgccatctat tttcccactg catcgcaaca gctaacggac
     4201 gagatctgcc aacgatgaac agagaaccgg tctctacatc caatgcggaa cattatattt
     4261 ggggtcaggt ttgcgatggc tggcacttac tcaggcaaga tgcattgagt gttatccaag
     4321 aacgggttcc tgctggcagg actgaggtga tgcactatca tacgtctgct cggcagttct
     4381 tttacgttct cgaaggagaa ggacaaattg cttttgaaga ccaaaccgtc acactgaaaa
     4441 aaggtgaagg gctagagatt cagcctcaag tctggcaccg attcgaaaac aactcccaaa
     4501 cagatgtaca ttttttagtc atctcatcac ccaaggcgca aggcgaccgg gtgaatgtcg
     4561 aataaattat ctatcaagga aatgaaatgt cgaacgtcaa agaagaactt ctgcagatgt
     4621 ataaaaaatc gcaagctgat atcacgtctc agcttgatga aatcgataag aaccgaaaaa
     4681 tttatccctt atggacagtt cgcgaaatca tagcgcatct ctccggctgg gacgactcta
     4741 ttacaggaat gttgaatgcg ctcctaaaag gcgatgtccc accaacccct gctgcccgcg
     4801 gtgtagatgt atacaatgct gaaaccgtta gcacacgtga aggcttgagt tacgatcatg
     4861 tcatccgtga gtattacaag acgcgcgaaa aacttttcgc actcattagt caagccaacg
     4921 acgacattct ttccaagcca tctatattgc cttggggtga agaaggaagt gtagcaatgc
     4981 ttgtgaagtt cttttcagag catgaagccg aacatgctga agatatcaag aaattcattg
     5041 aaacagccac acaaaaataa gccaaataac cgcagaaatt tctgcggtta ttttcttctc
     5101 aatacgataa aataaccctt catgaagtct cgcatccttg cgttactcaa catccgccgt
     5161 ggcgaagaaa agaacgtttt tcttatgctc acccaatatt ttttcatggg tgcagccatg
     5221 cttttcgtac agtctgcttc gctggcattg ttcttcactg cctgggatgc aaccgctatg
     5281 ccctatatct atctggggat cgcagtcatt gtatccacga tcacagcgat cttcctcaag
     5341 atatcagagc gcacctcact ggcgcgcttt ttgattctaa gcgttttgtt tgtcctcttt
     5401 ggaagcattg ctcttcgcat tgggctggct tttacaagtt caaaatggtt attgctggca
     5461 ttgcccattt ggtcgcagac gttggtcaac atcaccgtca cagccttttg gactctggct
     5521 ggcaacatct tcgatgtgcg ccaaggcaaa cgcatctttg ggttgatgaa tgcgggttcg
     5581 tggctctcgt atgtcgtcct cggacctttc actaccccgc tggtaaattt aatcggcaca
     5641 gaaaatctat atcttgtcat tgcggcttgc ttgttaattt cattctttct gcaacaagct
     5701 gttattcacg ccaacccccg cacagagaca aaccaagaaa ccactgatca acaatcgcaa
     5761 caaacttcca ttgcacaact ttttcgcatt cgttatattg ctttgatctt tgccctcatc
     5821 acgatttggc gcattgcata ttttattctc gataacattt tctacgaccg cgccgctttg
     5881 caatacccaa ctgcagattc tatggctggc tttattggcg gatttttcgc tattactggc
     5941 ttgcttggtt tcatcactga tatgttcctg acggggcgca tcatttctcg tttcggcttg
     6001 cgtgctggtc tcttagccac tcctacgctg acactgctct tcacagcagc actcgctgcc
     6061 actggcacac tcaaacccga aatgcttcct gctattttct ggctggcagt cgcaggcaaa
     6121 ttcaccaatg aaggtttggg cttttcatta gaccaaactg ccgataactt gctctaccaa
     6181 cccatccacg aacggattcg cgcccgtgct caaactgtga ccgaagggat tatccaacca
     6241 ttggcaatcg ggttggcagg cggcatgctt ttacttttca acacagcgct taaatttaat
     6301 gccatccaac ttgcatacat ttacattgcc ttcgccataa tttggatcat tatttgcata
     6361 gcacttatcc gcgcatatcc tgtggcactt acagaagcct tgcacaaacg acgttttggc
     6421 gacaagggga ttctcctaac ggatgctagc agtattcaga ttgtccgcat ggcattgaaa
     6481 agccagcatg catctgaagt tctgtatgcg cttgacctgt tggaaaaagg ggaaagtgat
     6541 acttttatag atgaccttga aacacaaatc cgctcgccca tgcctgaagt gcgaaaagat
     6601 gtcatccagc gcatcgaacg tttgaagatt accaaactgc tacgagatat tcagattcag
     6661 ctacgcacag agactgaccc caatgtacga gaaatcgctt cacgcgcact catcgtcctt
     6721 ggcgcagact caagtactgc tctgagtctc ctcgcccaag aagatgctgc tacccaacgt
     6781 ggcgctatga ttggcgcctt aagaatattg agagagtcaa aagacagcaa cgcttatgaa
     6841 cgccttttgg aattgacaaa atctaagcag gttcaagatc gggtttatgc agcacagtta
     6901 atggctgaag ctgcaaatcc cattctttcg aatgtgcttt tagccctttt aagcgatgaa
     6961 gacttacaag caaaaaaagc tgctttacgc gcagcagcta aattccagca cccagattta
     7021 tattcaaaga tcatccctgc tttagggttg ccactgacac gtagccttgc tttcaacgca
     7081 cttgtcgaag gcggaaatca tgcaattcca tctattattg aagctttgaa agatataagt
     7141 ctacacaggc gcatccactt gcgtttgatt cgcgcctgct ctcatatcaa gagtgaagca
     7201 gcaattcacg ttctggaagg gttaattcac cactcgaatg taagcgtccg ttcgcgagcg
     7261 ttaaccgctt tgcacgattg cagatacaag cccgagggag aatccgcttt tggggttagt
     7321 gaacaggtca cggcggagtc cagacgcgct gcttggttgt tgggagtcat gcatgatttg
     7381 ggcaaatcca ccaaaactga agtcttggtg cgggcagcga gtcatgatct ggatggcacg
     7441 atagaccgcc tctttcatct ctttgagttt atctatgagc cacaagcgat tcaacgtgca
     7501 caacacgcca ttcagcgaaa agatgcaaac cgcaaatcct atgcaattga agtgctagat
     7561 acaactctca gcaaaccaca taaggcagct tttattccat tgctggaaga ttttgcgccc
     7621 aaagaaacgc tagagaaaat gggaagtgaa tacaagcaga ccttcctacc gccagccggg
     7681 cgcgttatgg atattttgac caatccgctg gcaaagcaaa acccatggct ggttgcaact
     7741 gcgattgaat ttgcagatag catgggaatt tcgttagaca gcgaagtgac agattcatta
     7801 tcttcttcta acgagtcttt actagcgcgt atggttctgc aaaaaagaaa ggaaaagacg
     7861 atgttatcca ctgtagaacg agtgattgtt ttaaagtcgt tgaacatgtt cgaaaaaaca
     7921 ccagacgaag ctctcgccga gttggcagag cttgtgcagg aaatggttgt acaacccgga
     7981 gatgagatca tccgtgaagg agaaactggc gacagtttgt atattatcgt ggacggcaaa
     8041 gtagaagtgg tggatgataa ccgcatcttg aatcaacttg gagcacgggc tgtctttggt
     8101 gagctttcca tgctagactc ttcaccacgc actgcaacca tccgcgctat agaagagaca
     8161 tccctcttgc gccttgatca aacgtcgttt tatgaaatta tgagcgatta tgtagaagta
     8221 gccatgggca cgattcaatt attaacccgt aacttacgtg cccgcacggg tgatgtaatg
     8281 gaactaagcc gaatgttagg tcaatagaga aggagagaaa atggaaacca aacctgcttt
     8341 agactcaaaa cttgttgaag agttcgttgg aaattcacat gggaatttcg cgcgtgtgaa
     8401 agaattgctc gaacaagagc ctgcacttgt caatgcaacc tgggattggg gcggcggaga
     8461 ctttgaaact gctcttggtg ctgctgctca catgggcaat aagcagatcg ccaattacct
     8521 acttgagcac ggtgcgcggt tggatatttt tgccgctgcc atgctggata aattagatgt
     8581 ggtcaaagct gcattagaag catatcccga agcagtcaac acgccgggtc cgcacgggat
     8641 tccgctttac gtccatgcag aagcaggaga cgcgaagtct gttttggagt atctcaattc
     8701 ccttgaggga tagtaccaaa gggttcttgg actgaacaaa ttgggtcgat ataatttaga
     8761 aatactttag gagaaaaaat gaaaaagaga aatcaagtag ttataggtct gattgccgcg
     8821 atcatcatgc tcgcatgtgc ttgccccgtt tcaggtttgc cttttggtag tgaagaacaa
     8881 cccacacctg ggcttcctat tttgccagtt gcaaccatag tagatgcgcc tgccacagaa
     8941 gccccagtac aacctgctgc aaatgttttg ctcagtgacg attttagctc ccccagcgcc
     9001 gaaatggaaa cttattcagg cgatggcggc agtgctggaa ccgaaaatgg tgtgtatgtc
     9061 gtccgctcta cagctgacct gtggcaatgg ggtaagtcca attcgcagtt tgataatgca
     9121 atctttgaag cagatataac catgattgca ggtccgacca ataacaacgc tgggtttggc
     9181 attatctgtc gcttgagcga acgtgatgac acatccatcg atggctatat gtttgcgatc
     9241 agtggagatg gttattacac tattcgccat attgtctcaa gcaacatgac tgcactggta
     9301 gactggaccg aatcaagtgt gatcaaccag ggcaacgcct cgaatcacct gcgcgccacc
     9361 tgcaacggca gtgacttgag actcgaagtc aatggacaag aagtcgcttc cgctatcgcg
     9421 ccggcagatg gtccaaccaa cggaaccatg gctttcgccg ccatctcttt cgaaaccgat
     9481 cagccctatg ctgaaggtca ttttgataat ttgacagtca gccagccgta ataattagta
     9541 gaaataaaaa agacgtgctt ggcacgtctt ttttatttct actttgcacg cattttctgc
     9601 cgaagaaatg ctgcctcaaa gatacggtca aagaagcctt tatacccatc gttatcatac
     9661 aagatcatat aatctgcagg accatattct ttaggtggca gcataatagc aggattgggg
     9721 ttgatctcca aaataaacaa ctcgcctttt tcgttcatac ggatgtcgca gcgtccataa
     9781 ccagaaatac ccattgcctg aaacattcgt tttccttcat tttgaagttt tgcctttagt
     9841 tcaggttcgg tcacttcttt aaaatcaaag ggaatgttgt aatcccactt aatatcggta
     9901 tgccaaaact cttccccatc tgggaagatc aactcggcag gcgggtaaac gaagggattg
     9961 tccatatcag ccgggttgtc aaccaccaac acgttgaact cttttccaat aataaattct
    10021 tccatgcggg cggcaccgaa ctcgctacaa actcgtttta cctgtgtgag aacttgctca
    10081 agagtatcag ctcgcgagtc tttgaacatg ccggtactac cataactctt gggatgtttc
    10141 accataatag gatagcgcaa atttttgaca aggtgttcag cttcttccac gctatgaaca
    10201 cgatagccct ttgcaaaacc gactccattt gcatcagcga tcgtttgcat ttcttcacgg
    10261 gttgggtcaa agcagcgaga gtctgcgccg gtaaaaggta aattgagcgc ctccagagct
    10321 tctacaactt cacttgcttc atatccaagt tcttcatctt cctcgtcttc aaattcgtat
    10381 ccttcacaga catttaaaaa gacatcaaac tctccagatt cagccaacgc tttgatcttc
    10441 tcttccactg gggctgtcaa cgtgaccatt ttccagtcgt aatcttttag ataaggagca
    10501 gggttgaaat cttcaatgta ttcatcagac aaaacacaga ctcgcatagg gtctccaaat
    10561 ttttgaagga tacgcaaata acagctacac aaacgccgct gtctaaatgc gtttgcgatt
    10621 atccacccat ttccgattca gcaattgatt ttttaagaaa aatagagcag attcctctgc
    10681 tctatttggt ttccgtcacc ttgcggatgc tgcgtggctg ctctgtgttg taccccttgg
    10741 cgagggtcaa atgataagca aacaattgtg caggcaaaat gctcaccaat ggagaaagcc
    10801 attctggcgt atcaaccgga atctgcaatg gaacttgcgc caacaacaga gccttccggt
    10861 cgttcgagat caccaccaac tcggcagcaa tatcagaacg caggcgtttg agcatctcaa
    10921 gcatcgaatc aaagacctta cctctggctg ccactgccaa aacagggtag ccgctctcca
    10981 ccatggcaat cggaccatgc gcaaaatcgg cagaagaata tggctcggca atgatatatg
    11041 tgagctcttt tagtttcaat gcccattcaa atgcggtcgc ataattaaac ccgcgcccca
    11101 agacaaccgt ttg
//
LOCUS       DAOQJE010000006        10585 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_3242_length_10585_cov_6.868756, whole genome shotgun sequence.
ACCESSION   DAOQJE010000006 DAOQJE010000000
VERSION     DAOQJE010000006.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 10585)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10585)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10585
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3242_length_10585_cov_6.868756"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..319
                     /locus_tag="PLT08_00280"
     CDS             <1..319
                     /locus_tag="PLT08_00280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE02923.1"
                     /translation="YPDEVRKEGMATGATYLDVNPMILKALQEKGVDPYIVYEQGLRN
                     EIDKKTLRIDFVKINVSDALTEWQLKDHSKAPIPIRLAIWLNNNAATYGYIQDGNSWV
                     LKQ"
     gene            complement(405..1406)
                     /locus_tag="PLT08_00285"
     CDS             complement(405..1406)
                     /locus_tag="PLT08_00285"
                     /inference="COORDINATES: protein motif:HMM:NF014069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNE02924.1"
                     /translation="MVKNNNSTILIVDDDAAGREVLVSILESDGYELILAENGFQAIE
                     LANAIHPDIILLDIMMPGMDGFEVCRRIRSEKDIAEVPILFLSALDDRQSLLSGLEAG
                     ADDFINKPPDRYELRARLLGISRLNRYRKLLDERQNLEEAHQQLLLAYDKTIEGWSRA
                     MDLRDKETEGHTQRVTQTTLKLAKFFGIQGEELIHIQRGALLHDMGKLGIPDAILLKP
                     DKLDDDEWKLMCQHPQMAYDMLYPIEYLRPALDIPYCHHEKWDGTGYPRGLKGEEIPL
                     SARIFALADVWDALTSNRPYREAWSEQKTLAYIRQQSGKQFEPRVVEAFTKIISDSQ"
     gene            complement(1430..>2586)
                     /locus_tag="PLT08_00290"
     CDS             complement(1430..>2586)
                     /locus_tag="PLT08_00290"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hybrid sensor histidine kinase/response
                     regulator"
                     /protein_id="HNE02925.1"
                     /translation="FISKPFDRAELRARVRSITRLNTYRLLYERNMMFAWISDQSNDG
                     YLLVRNGDEVIYANPRARFYLGMDLDVNIPSGSSFMGIASRQYRPQPIEAWKTWPKPA
                     SSNEPRVRYLVRPETTSSHEFWLEASVFETPKGEVGSRIIRLRDVTADILNRRNTRGF
                     GEAITHKIRTPMTHMISSLDLLVKHLPKLSQEEAMHLLETAFMGAKRLSDTMDRILKY
                     SNLYTYTNHSECFSLAYFNEIAERIAGEVGIENMQVHIDEAISQTKIVMPLQSVEVLL
                     WEILDNSKKFHPSQMPTVKIDVLMNASKNIQIQISDDGMALSPKQLTTAWLPYYQGEK
                     DFTGEMPGMGLGLSTVNAIVWGAGGTSQMLNREDKPGVVVRLSIPIAKNA"
     assembly_gap    2587..2686
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2687..3042)
                     /locus_tag="PLT08_00295"
     CDS             complement(<2687..3042)
                     /locus_tag="PLT08_00295"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNE02926.1"
                     /translation="MKPTILIVDDEPSGQQVVESILQDQGYTMEFASTGKEAIKKAGE
                     LKPDLILLDIMLPEIDGIQVCQTLRKNTELAKIPVVMLTALNDRDIRIASLDAGADDF
                     ISKPFDRAELRARVRS"
     gene            complement(3170..3820)
                     /locus_tag="PLT08_00300"
     CDS             complement(3170..3820)
                     /locus_tag="PLT08_00300"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HNE02927.1"
                     /translation="MIHILIAEDHLMVRAGIRALLEKVGDMVILGEASNGQEAIDMTE
                     SLNPDVLIMDIMMPRLNGIQAAEQLRSRKHRVNILLLSMYADEGLVYQALQSGANGYV
                     LKSSASDELVEAVREIANGKKFLSGPISEIVMENAFKPRPANEVDPLASLSPREKEIM
                     QLIAEEHTSVEIAQFLSISEKTVEKHRANLMEKLHVRNLAGLVRLAVKYRLVDRDS"
     gene            complement(3817..4887)
                     /locus_tag="PLT08_00305"
     CDS             complement(3817..4887)
                     /locus_tag="PLT08_00305"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNE02928.1"
                     /translation="MNSIILIVDDNEASRETLESILASQNYDLHMAADGLQALEKANA
                     LFPDLILLDVMMPGMDGFEVCRRLRSTPALAEVPIILLTALDDTASRLEGIEAGADDF
                     ISKPFDRQELRARVRTITRLNRYHALLEQRENLREMAQRMVTGLEDERMRISRELHDE
                     LGQLLTAELMDIRDLQNDLTIPPVVLFERLEKIHAQAYEVTVKIRRLAHELRPPLLST
                     LGLKQAVETHCSKFSNHSHIPITVEVDDAVSALPDVYGIILYRVLQEALTNIAKHANA
                     DHAWVELSLEDNHINLIVQDNGRGLPTTETAASGIGVVGMKERAALVGGELSIRSGKN
                     GGTILSVSLPLKPGTTVKREGA"
     gene            complement(4884..10571)
                     /locus_tag="PLT08_00310"
     CDS             complement(4884..10571)
                     /locus_tag="PLT08_00310"
                     /inference="COORDINATES: protein motif:HMM:NF024583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HNE02929.1"
                     /translation="MKQSEAALQASEANYRNLVESSESAIAVVDKNGKILFVNLTGND
                     IWKDRNLVGKSIYDLFPPEYAERYSQAIHSVINAQIGTLDEMQSVIAGKLMWFRLSMT
                     PLKNPDGSISTLLINAWDITDRKVAEQSLRESEEKYRTLIDATSDGVFVAQDERFVFC
                     NKSLPAMLGYTYQEFENIPFERVVLKEHLDVWIDRFRQRIHGESVVSNYQVQFQNKTG
                     MEHIWVELRANKIEYGGRPAVLGIVRDITEQKNILTEIENLAKFPAENPGPVLRIDMY
                     GKLLYANDASFDLLNEWNLEIGKQAPAIIQKVAAEVVETEIDKTIDTTHNGRVVTLNF
                     ILLRQKGYINIYGRDITEQRHAEKEVHALQEFSQAIIDALSSHLCVLDERGVIISVNQ
                     AWRKFAEENPPIPLNYGIGENYLTICENAKGDYENDALVVSRELRAVLEGQKSEAYIE
                     YPCHSPEEDFWFYLSIRRFESKGSTRLLLLHENITHRKQMETEILNVNQRFTELAENV
                     SDIFWVSDPVTRRNVYVNPAFSKVTGMSMDAVENLPNDFLDIVLPEDRNTIIKNRELE
                     ESGFKTDSKYRIRRPDGMIRWIHDRATPVIDSSGRVIRIVGISTDITERVDAERRLSE
                     SEIRLRQIAEAMPEAFWMVDDKTKKFVYMSPAYKTIWKRSSEELLDGGNQAFMDTVLP
                     EDRAGLSASFIRQEHGEPTEMEYRIVRPDGSMRWIYDRSFPIFDEETGRLLNTAGVAA
                     DITEQKLAGEALKDSEERLRLSLAAAHQGLYDLNIQTGDAVVNREYVEMLGYDFETFK
                     ETFQNWENRLHSEDREIALHAFSEYVNGNSPEYQVEFRQKTKDGSWKWILSMGKIVEY
                     DAMGKPLRMLGTHTNIDANKQAEFELKRRAAQLALINDVGRKIAGVLDIHGVLDLSAR
                     LVQEYFDYHHVGLFTLDETKSHMLMRAKAGKYAELFPNEHSIPLGEGIVGSVGANGEM
                     ILTNQVTESETYRNFFPKVVHTASELGLPIKIAGQVIGVLDVQSPVPNAFTSNDIQVL
                     QTLADQVAIALENARLYESSQQELTERQKLIRDLEMLVEAGRALNESLDPKSIYPLIY
                     YYLSKVMPCDFVIVSSYDPKNSLIQCEFMHSSEGPQDVSGFPLIPLEPPERGTQSRVI
                     RSGTSLLLPDYEAALRTASNVLYFDEKAEISTQVDEDEERTRSAIIVPLKVNGIVTGV
                     LQVLSTKLYAHNHDHLRFTEALAFHVSAALSNAQLFSELEKRVRERTAEVQDLYDNAP
                     AGYHSIDPSGRIILINQTELNWLGYSREEIVGHHISKVLTDAGLAKFQENFPVFKYNG
                     IIKDLELEMRRKDGSTLPALISATAIRDQEGNYLMSRSTVFDNTERKMADQALREREE
                     VYRALFENANDAIFMLKPDGTIIGANPHCMDILGIPYSELPGRNALDFIVNTEFQAAE
                     GQLRRLLAGERLPIYERTFKRKDGALIEAEINLSLISDEAGKPKFIQSVVRDISTRKR
                     AEETLHLANAEMQRALRLKDEFLANMSHELRTPLNAVLGITESLLEQISGPLNEKQKK
                     YLQTILESAQHLLELINDILDLAKVNAGRIELDINKIDIQTFAQSNLRMVRELAQKKN
                     IKVNLEIAPTVKSAWADERRLKQMLVNLLSNAIKFTPSGGDIGLEINGDRTEHTVNFV
                     VWDTGIGIKEDDLHLLFKPFVQLDAGLARGSQGTGLGLALVSQMARLHGGRVLVESEP
                     NKGSRFTITIPWVELSNTGPLTFPINSMPLPVEVQPEKNISQDYQILLVEDTESVTML
                     IGDYLKKHGYKIMTARDGFEGLARIREVKPDLILMDVMMPEMDGIETTRRIREQLELK
                     DIPIIALTALAMAGDRERCLEAGMNDYMSKPVKLKELLSIIEKYLKPGKEGTQ"
BASE COUNT         2990 a         2511 c         1959 g         3025 t
ORIGIN      
        1 atatccagat gaggtgcgta aagaagggat ggcaacaggg gcaacctatt tggacgtaaa
       61 cccaatgatc ttgaaagctt tacaagaaaa gggggtagac ccttatattg tttatgaaca
      121 agggttgcgt aatgaaatcg ataaaaagac tctcagaatt gattttgtga aaatcaatgt
      181 atcagatgca ctaactgaat ggcaactgaa agatcatagc aaggctccca tcccaattag
      241 gctggcaatt tggttaaata ataatgctgc tacctatgga tatatacagg atggaaatag
      301 ctgggtatta aaacaataac tggtttaaac tttaacaaac tatataaaga tactatattc
      361 ttgttaagaa gaataaaaat aaggcaacaa atgttgcctt atttttattg cgagtctgaa
      421 ataatttttg taaaagcctc tacaacccgc ggctcaaatt gctttccaga ctgttgccga
      481 atataagcta atgttttttg ctcgctccat gcttcacggt aggggcggtt tgatgtcagt
      541 gcatcccaaa catcggctaa ggcaaagatg cgagcggaaa gtgggatctc ctcacctttc
      601 aaaccacgtg gataccccgt accatcccat ttttcatggt gacaatatgg gatatcaaga
      661 gcagggcgaa gatattcgat cgggtaaagc atatcgtatg ccatttgtgg atgctgacac
      721 atcaacttcc attcatcatc atccagtttg tctggcttta agagaattgc atctggaatc
      781 cccaatttac ccatatcatg taacaaagct ccacgttgaa tatgaattag ttcttctcct
      841 tgaataccga agaatttggc aagcttcaga gtggtttgtg taacacgttg agtatgcccc
      901 tccgtttcct tatcgcgcaa atccatggca cgagaccagc cttcgatggt tttatcatag
      961 gctaaaagaa gttgttgatg cgcttcttca aggttctggc gctcatctag taattttcgg
     1021 taacggttca atcgagaaat gccaagtaat cttgctctta attcatagcg atctggcggc
     1081 ttgttaataa aatcgtctgc ccctgcttct aacccactta acagagattg gcgatcatcc
     1141 aatgcactaa gaaataaaat cggaacttca gcaatatctt tttcgcttcg gatccttcga
     1201 caaacttcaa aaccatccat acctggcatc ataatatcca aaaggataat atcgggatga
     1261 atagcatttg caagctcaat agcttgaaat ccattctctg ctaaaatcaa ctcataacca
     1321 tctgattcaa gaatagatac taaaacttct cttccagcag catcatcatc tacgatcaaa
     1381 atcgtactat tattattttt caccataacc tccaatactc tataaccttc taggcatttt
     1441 tggcaattgg aatagacaga cgaaccacaa cccccggttt atcttcacga ttcaacatct
     1501 ggcttgtccc accagcgccc cacacaatag cattaactgt agataacccc aaccccatgc
     1561 ctggcatttc gcctgtgaaa tctttttcgc cttgataata tggcaaccat gcagtcgtta
     1621 attgtttcgg cgacagagcc attccatcgt cactgatttg aatttggata ttttttgagg
     1681 cattcataag tacatcaatt ttgacagtag gcatttgaga tgggtgaaac ttttttgaat
     1741 tatccaaaat ctcccacaac aagacttcta cagactgcaa tggcatcaca atctttgtct
     1801 gacttatagc ttcatctata tgaacctgca tgttctctat ccctacctcg ccagcaattc
     1861 tttccgctat ctcattgaaa taagctaaag aaaagcattc agaatgattg gtataggtgt
     1921 aaaggttcga atattttagt atcctatcca tcgtgtcaga taaacgctta gcacccataa
     1981 atgcagtctc taacaaatgc attgcctctt cttgagataa ttttggcaaa tgctttacaa
     2041 gcaaatcaag gctcgaaatc atatgcgtca ttggggttcg tatcttatga gtgatcgctt
     2101 ctccaaaccc tcgagtgtta cgtcggttta ggatatcagc cgtcacatca cgaagacgaa
     2161 taatgcgaga cccaacttca ccttttggcg tctcaaaaac cgatgcttcc aaccagaatt
     2221 catgagaact cgttgtttca ggacgaacca gataccgaac acgcggctca ttactagacg
     2281 caggcttggg ccaagttttc caagcttcta ttggctgtgg gcgatactgc cgagaagcaa
     2341 ttcccataaa ggaactacca gatggaatat tcacatccaa atccatacca agataaaaac
     2401 gggctctcgg gttggcataa atgacctcat ccccatttcg aactagcaaa tatccatcat
     2461 tcgattggtc gctaatccac gcaaacatca tatttcgttc atataaaagg cgataggtat
     2521 ttaggcgcgt aatcgaacgt acacgagcac gtaactctgc ccgatcaaaa ggcttggaaa
     2581 taaaatnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2641 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnatcg aacgtacacg
     2701 agcacgtaac tctgcccgat caaaaggctt ggaaataaaa tcatcagctc cagcatccaa
     2761 actggcaata cgaatgtcac ggtcattcaa agcagtcaac atcacaactg gaatcttggc
     2821 aagttcagtg tttttacgca acgtctgaca aacttgaatg ccgtctattt caggcagcat
     2881 aatatctaaa agaatcagat ccggctttaa ttcccctgct tttttgatag cttcctttcc
     2941 tgtggaagca aattccatcg tatacccctg atcttgcaat atagattcga ctacttgttg
     3001 cccagagggc tcatcatcaa ctataagaat ggtaggtttc attcatatct gtcctcaaac
     3061 ataaatatgg atacatatac tactaaccta accaaggata tttcaactta tcctacttga
     3121 ggatacagga aaacccctat tgtgaagagg cttccagtat tgtaaacaac taggaatccc
     3181 tgtcgacgag gcgatatttc actgcaaggc gcaccaaccc cgccaggttt cgtacatgta
     3241 atttttccat caagtttgca cgatgttttt ccactgtttt ctcactaatc gagaggaatt
     3301 gtgcaatctc cacacttgta tgttcttctg caattagttg cataatctcc ttttcacgag
     3361 gagataaact agcaagcgga tccacttcat ttgcagggcg cggcttgaag gcattttcca
     3421 tcacgatttc agaaatggga ccacttaaga attttttacc atttgcaatt tcacgcacgg
     3481 cttccaccaa ttcatcactg gcagaagatt tcaacacata tccatttgcg ccactttgca
     3541 aagcttgata taccaagccc tcatctgcat acatggatag cagcaggata ttgacccggt
     3601 gctttcgact ccgcagttgt tctgctgctt gaataccatt tagacgaggc atcatgatat
     3661 ccataatcag aacatctgga tttagagact cagtcatatc tatggcttcc tgcccatttg
     3721 aggcttcacc aagaataacc atatcgccaa ctttctctaa cagtgcacga atccccgcac
     3781 gtaccatcaa atgatcttcc gcgatcaaaa tatgaatcat gctccctctc tttttactgt
     3841 agttccaggc tttagtggca aagatactga aagaatggtg ccaccattct ttcctgaacg
     3901 aatacttaat tccccaccca ccaatgcggc tcgctctttc atacctacaa caccaatacc
     3961 gcttgcagca gtctcagttg taggtaagcc gcgcccatta tcttggacaa tcaaattgat
     4021 atgattatct tccagactta actctaccca ggcatgatcg gcattcgcat gtttagcaat
     4081 attggtcagt gcctcttgta aaacccggta caaaataatt ccatacacat cagggagggc
     4141 tgagacagca tcatctactt caacggtaat gggaatatga ctatgatttg aaaatttaga
     4201 acaatgcgtc tctacagctt gtttcaagcc taatgtactt aacaaaggcg gacgtagttc
     4261 atgcgcaagc cttcgaattt ttacggtaac ctcatatgcc tgtgcatgga ttttctctaa
     4321 tcgttcaaac aaaacaacag gcggaatagt gaggtcgttt tgcaagtcgc ggatatccat
     4381 caattcagcc gtaagcagtt gacctagttc atcatgaagc tcacgtgaaa tgcgcatacg
     4441 ttcatcttcc aaccctgtca ccatacgttg tgccatctcg cgcaggtttt cacgttgttc
     4501 tagcaaagca tggtaacgat taaggcgagt gattgtccgt acgcgcgccc gcaattcctg
     4561 ccgatcaaat ggtttggaaa taaagtcatc ggcgccggct tcaattcctt ccaaacgaga
     4621 ggcggtgtca tctaaggcgg tgagaaggat gatagggacc tccgcaagcg ccggcgtaga
     4681 ccggaggcgt cgacaaacct caaatccatc catccctggc atcattacat ccagtaagat
     4741 cagatcgggg aataaggcgt tcgctttttc caatgcttgc aaaccatctg ctgccatatg
     4801 cagatcataa ttttgcgagg caaggattga ctctagcgtc tctcggctgg cttcattgtc
     4861 atctacgatc aaaataatgc tgttcattgg gtgccctcct tacctggttt tagatatttt
     4921 tcaataatgc tgaggagttc ttttaacttg acaggcttgc tcatataatc attcatgccc
     4981 gcttctaggc aacgttctcg atcgcctgcc atagccagtg cggtaagcgc aataataggg
     5041 atatctttta gttctaattg ttcgcgaatg cgacgggtag tttcaatacc atccatctct
     5101 ggcatcataa catccattag gatcaggtct ggttttacct cgcgaattcg ggcaagccct
     5161 tcaaaaccat cgcgggctgt cattatttta tagccatgtt tcttaagata atcaccgatc
     5221 aacatcgtaa cagactctgt gtcctccact aaaagaattt ggtagtcttg actgatattc
     5281 ttttcaggtt gcacttccac tggcaatggc attgagttta tagggaatgt aagtggtcca
     5341 gtattactca attctaccca tggaatggta atcgtaaaac ggcttccttt attcggttca
     5401 ctctctacta ataccctacc accatgcaag cgtgccattt ggctgacgag tgcaagccct
     5461 aaacctgttc cctgacttcc acgtgccaat ccggcatcca attggacgaa aggtttgaaa
     5521 agcaagtgaa ggtcatcttc ttttatacca atgcccgtat cccatacgac aaagtttacg
     5581 gtatgttccg ttctatcacc attaatctca agcccaatat ctccgcctga aggagtaaat
     5641 ttaatcgcat tactcagcag gtttactaac atttgcttaa gacggcgttc atctgcccaa
     5701 gcagacttta cagtgggcgc aatttccaga tttactttga tattcttctt ttgcgctaat
     5761 tcacggacca tacgcaagtt tgattgagca aaggtctgaa tatcgatttt attaatatcc
     5821 aattcgatgc gacctgcgtt gactttggca aggtccagaa tatcgttgat caactccaat
     5881 agatgttgag cgctttcaag aatggtctgg aggtatttct tttgtttttc gtttagagga
     5941 ccggaaattt gttcaagtaa gctttctgta atccctaaga ccgcattaag cggtgttcgc
     6001 aattcgtggc tcatatttgc caaaaattca tcttttaggc gtaaggcgcg ttgcatttca
     6061 gcattagcaa gatgcaatgt ttcttcagct cgtttacgag tggatatatc gcggactacg
     6121 ctttgaataa attttggttt tccagcttca tcagagatca aggataggtt aatctctgct
     6181 tcaatcaaag caccatcttt ccgtttaaaa gtccgctcat aaattggcaa tcgttctcca
     6241 gctaacaaac gtcttaattg cccttcagct gcctgaaatt cagtattcac gatgaaatca
     6301 agagcatttc tgcccggcaa ttcagaatat ggaatcccca aaatatccat acagtgcggg
     6361 tttgccccaa taattgttcc atctggttta agcataaaga tggcgtcatt ggcattttca
     6421 aacaaagcac gatagacctc ttcgcgctca cgaagtgcct gatctgccat ttttctttct
     6481 gtattatcaa acactgtaga acggctcata agataattac cttcttgatc tcgaattgcc
     6541 gtagcactga tcaacgctgg caaggtagag ccatctttcc gacgcatttc aagctctaag
     6601 tctttaatta tgccgttgta tttgaatacc gggaaattct cctgaaattt tgccaaccca
     6661 gcatctgtta atacctttga aatatgatgc cccactattt cttcgcggga atagccaagc
     6721 caattgagtt cagtttggtt aataagaata attcgcccgc taggatcaat cgagtgatac
     6781 ccagctggag cgttatcata cagatcttgt acctcggcgg tacgctcgcg cacccgtttt
     6841 tcaagttcag aaaacaattg cgcattcgat aatgcagcag aaacatgaaa tgccaaggct
     6901 tctgtaaaac gcaaatggtc gtgattatga gcatataact ttgtacttaa aacttgtaaa
     6961 acgccagtga caatcccatt gacctttagg ggaacgataa tcgcagaccg agtccgttct
     7021 tcatcttcat ctacttgagt agaaatttct gctttttcgt caaaatacaa aacatttgaa
     7081 gccgttctaa gagccgcctc ataatcagga agcagtagag acgttccact gcggataact
     7141 ctgctttgtg tgcctcgttc tggcggctcg agaggaatca atggaaaacc agatacatct
     7201 tgtggtcctt cagacgaatg cataaactcg cattgaatca ggctattttt ggggtcgtaa
     7261 gacgatacga tcacaaaatc acaaggcatg actttagaaa ggtaataata gatcaacgga
     7321 tagatcgact tgggatcaag tgactcgttc aaagcacgcc cagcttcgac caacatttcc
     7381 agatcacgaa tcagcttttg ccgttcggtc aattcctgtt gactagattc ataaaggcgg
     7441 gcgttttcaa gcgcaatcgc tacttggtct gccaacgttt gcagaacttg aatatcattt
     7501 gaagtaaacg cattggggac tgggctttga acgtctaata caccaatcac ctgcccagca
     7561 atcttaatgg gcagtcctag ttcagaggca gtatgaacca ctttggggaa gaagtttcga
     7621 taggtttcgc tctcggtaac ttgattggta agaatcatct ccccatttgc ccctacagaa
     7681 ccaacgatac cttctccaag cggaatactg tgttcatttg ggaaaagctc ggcatacttc
     7741 cccgctttcg cccgcatcaa catatggctc ttcgtttcat ccaacgtaaa caagcctaca
     7801 tgatgatagt caaaatattc ctgtacaagg cgcgcagaaa ggtctaacac accgtgaata
     7861 tcgagcaccc cagcaatttt tctacccaca tcattgatca acgccaattg agccgctcgg
     7921 cgttttagct caaattctgc ctgtttattc gcatcaatat tcgtatgcgt tccaagcata
     7981 cgaagcggtt ttcccatggc atcatattca acgatcttgc ccatggaaag gatccacttc
     8041 cagcttccat ctttcgtctt ctgacgaaat tcaacttggt attccggaga gtttccattg
     8101 acatattcag agaacgcatg aagggcaatc tctctatctt cagaatgcag acggttttcc
     8161 caattctgaa aagtctcttt aaaggtttca aaatcatacc caagcatttc aacatactcg
     8221 cggttaacaa ccgcatcccc ggtctgaata ttaaggtcat aaagaccttg gtgagcagct
     8281 gcaagtgaaa gacgcaacct ttcttcacta tctttcagag cttcacctgc taatttctgt
     8341 tctgtaatat cagctgccac accagcggta ttcaataacc tacctgtttc ctcatcaaag
     8401 atcgggaaag accggtcata aatccaccgc atcgaaccat caggtctgac gattcgatat
     8461 tccatttcag tgggctcacc atgttcctgt cttatgaaag atgcgcttaa gccagcccga
     8521 tcttcaggca aaaccgtatc cataaaagcc tgattccccc catccagtaa ttcctctgaa
     8581 gaacgtttcc aaatggtttt ataagcaggg ctcatgtaaa cgaacttctt tgtcttatcg
     8641 tcaaccatcc aaaacgcttc tggcatggct tctgcaattt ggcgcaaccg tatttccgac
     8701 tcactcaaac gtcgctctgc atctactctt tcagtgatat ccgttgaaat gccgacaata
     8761 cggatgaccc taccagacga atcaatcacc ggagttgccc tatcatggat ccaacggatc
     8821 attccatcgg gacggcggat tctatacttc gagtctgttt tgaaccctga ctcttccaac
     8881 tcacgattct taataatcgt gtttctatct tctggcaaaa caatatccaa aaagtcattt
     8941 ggaaggtttt caaccgcgtc catactcatc ccagtcacct tggaaaatgc cgggttgaca
     9001 taaacattac gtcttgtcac aggatccgaa acccaaaaga tatcagatac attttctgcc
     9061 aattcagtaa aacgctgatt gacattcaga atttcagttt ccatctgttt acgatgagta
     9121 atattctcat gcaaaagaag cagccgagtt gaacctttgc tttcaaatcg gcgtatgctc
     9181 aaatagaacc aaaaatcttc ctctggtgaa tggcatggat actcaatata agcttcactc
     9241 ttctgccctt ctaaaactgc gcgcaattca cgagatacta caagcgcatc attctcataa
     9301 tcccctttag cattttcaca aattgtcaga tagttttcac cgatcccata gttcagcgga
     9361 attggaggat tctcttctgc aaacttacgc caagcctggt tgaccgaaat aatgacccca
     9421 cgttcatcaa gcacacacaa atggctagac agcgcatcaa tgattgcttg gctgaattct
     9481 tgtaacgcat gcacttcttt ttctgcatgc ctctgttccg tgatatcccg tccatagata
     9541 ttgatgtaac ctttttgtct caaaaggata aagttcaatg tcactacacg tccattatga
     9601 gtagtgtcaa ttgttttatc gatctcagtt tccacaactt ctgccgctac tttttgaatg
     9661 atagctggcg cctgtttacc gatctcaaga ttccattcat tcaataagtc aaaactagca
     9721 tcattggcat acaacaattt accgtacata tcaatacgaa gtacaggtcc agggttctca
     9781 gccggaaatt tagctaaatt ctcaatttcc gtaagaatat tcttctgctc cgtaatatca
     9841 cgaacaatgc ctaaaaccgc tggtctacca ccatactcaa tcttattcgc ccgtaactct
     9901 acccaaatgt gttccatgcc tgttttattt tggaactgaa cctgataatt cgaaaccaca
     9961 gattccccat gaatacgctg acgaaatcga tcgatccaca catctaaatg ttctttaaga
    10021 acaactcgtt cgaacggaat attttcaaac tcttggtagg tatagcccag catagcaggt
    10081 agagacttat tacaaaatac aaacctttca tcctgagcga cgaaaacgcc atcagatgta
    10141 gcatcaatca gagttcgata cttttcttca ctttcccgca aagattgttc cgctactttg
    10201 cggtcggtga tatcccatgc attgataagt aatgttgaga tcgagccatc tgggtttttg
    10261 aggggtgtca tgctcaaccg aaaccacatc agctttccag caataacact ctgcatttca
    10321 tccaatgtcc cgatctgagc attgattact gaatggatag cctgtgaata tcgttcagca
    10381 tattccggcg gaaacaggtc gtaaatagat ttaccaacca aattcctgtc tttccaaata
    10441 tcattccctg ttaaattaac gaataagatc ttgccatttt tatctacaac tgcaatggca
    10501 ctttcagagc tctcaaccaa attacggtag tttgcctcac tggcttgcaa cgcagcttcc
    10561 gactgcttca aatcagtaat atcaa
//
LOCUS       DAOQJE010000007        10235 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_3415_length_10235_cov_6.140668, whole genome shotgun sequence.
ACCESSION   DAOQJE010000007 DAOQJE010000000
VERSION     DAOQJE010000007.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 10235)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10235)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10235
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3415_length_10235_cov_6.140668"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(139..1056)
                     /gene="tatC"
                     /locus_tag="PLT08_00315"
     CDS             complement(139..1056)
                     /gene="tatC"
                     /locus_tag="PLT08_00315"
                     /inference="COORDINATES: protein motif:HMM:TIGR00945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatC"
                     /protein_id="HNE02930.1"
                     /translation="MRKFFKGVWWVITLPFRLVYAIIAFPFRAGYRFFKLLNSEPEER
                     PIADVFADLVTEKEVRDQLWEQIEAFRMHLLRSIIALSILVIAAFWVAEPAMAFLSEP
                     VGGLEKLQAIEVTEEIGVFMRVAMTIGIAIAFPYIAFEFWLFAAPGLKPREKKLGLVG
                     IPLATLLFLLGMAFTFYVLLPTALPFLGSFTSISQFWSAREYFSFVTGLMLWIGGFFE
                     FPLLIYVLTAIGLVNPKFLADQWRLAIVIIAVLAAAITPTVDPVNMGLVMVPMILLYF
                     ISIGLSYLAYAGRSKNAEVQEAASDVELG"
     gene            complement(1049..1456)
                     /locus_tag="PLT08_00320"
     CDS             complement(1049..1456)
                     /locus_tag="PLT08_00320"
                     /inference="COORDINATES: protein motif:HMM:NF014470.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase TatA/TatE family
                     subunit"
                     /protein_id="HNE02931.1"
                     /translation="MEILGIGMPELIFVVIIALLILGPKDMQKAGKTIGKFLRDIITS
                     DGWKLFQQTSRDLRTLPNRLIREANEDLDKVNREIKSAASAPDPARSAPANSRPQPQP
                     YSATSPIKPGGENVILPNPQEKNNPESETPNNA"
     gene            complement(1459..1917)
                     /gene="nusB"
                     /locus_tag="PLT08_00325"
     CDS             complement(1459..1917)
                     /gene="nusB"
                     /locus_tag="PLT08_00325"
                     /inference="COORDINATES: protein motif:HMM:TIGR01951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HNE02932.1"
                     /translation="MKPRTRARALALQVLYETDLSTHLPGDVLKTRLEETTLPQDLAE
                     FARSIIFGILPIRENLDRVIAKYAPEWPLDQIAAIDRNILRMACWEFAISRQTPVKVA
                     INEAVELAKLFGSDSTARFVNGVLGTLVEHEQEIYQVLKNEIPQETNLES"
     gene            complement(1922..2269)
                     /locus_tag="PLT08_00330"
     CDS             complement(1922..2269)
                     /locus_tag="PLT08_00330"
                     /inference="COORDINATES: protein motif:HMM:NF015721.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HNE02933.1"
                     /translation="MTESTQGKTTVSPEVLTTIASLAALEVPGVSRLAPVSGGVNRLF
                     RRGTSDGVRIEAKDNTVFVDLFLILKDGVNIREVGRNVQANVARSVQEMVGMEVGEVN
                     VHIEDIDFEGDEA"
     gene            complement(2283..3041)
                     /gene="fabG"
                     /locus_tag="PLT08_00335"
     CDS             complement(2283..3041)
                     /gene="fabG"
                     /locus_tag="PLT08_00335"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002442508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004316 - 3-oxoacyl-[acyl-carrier-protein]
                     reductase (NADPH) activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] reductase"
                     /protein_id="HNE02934.1"
                     /translation="MSFKLSLENKVAIVTGGSRGIGRAIALEFADRGAAVVVNYNKSP
                     ESAEEVVKKIQEAGGKAAAFQADVSDFKQAEALVKFAIDTFGDLHILVNNAGITRDQL
                     IMMMPEADWDAVINTNLKSTFNCSKAAVKHMMRKRTGRIINMASVAGQMGNPGQTNYS
                     ASKGGQIAFTKALAREVSARNITVNAIAPGFVDTEILDAMTPEALEAALKLVPLGRKG
                     KPEEIAFTATFLASDGAAYITGQVIGVDGGMAMM"
     gene            complement(3063..3923)
                     /locus_tag="PLT08_00340"
     CDS             complement(3063..3923)
                     /locus_tag="PLT08_00340"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNE02935.1"
                     /translation="MPLTNDQCLELFGSSQIDLIRMADAHTLNVQAWLSSRSPMAKTF
                     QGMGVRASSTGLKLPLLNLALGANFPDKTASSEIEAEVKQVIDFFAERNVPWYWWMNA
                     EPRPVNIAMVLEKFGCVYDAPPLPAMIAPTKQDVSSFPQVDKSIQVWRAASIKDLQMA
                     STIRRRAFQFTEGEALTYFEDMAQDWLSNESSARLFLAGPKKGEAVSIGAIIEAKGIP
                     GIYVMATLPEFHRQGYGKAILSRLLSEAAQCEGKIVALTASSAGAGLYAQFGFRHLFD
                     FNFYSIPDEV"
     gene            complement(4046..4720)
                     /locus_tag="PLT08_00345"
     CDS             complement(4046..4720)
                     /locus_tag="PLT08_00345"
                     /inference="COORDINATES: protein motif:HMM:TIGR01906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01906 family membrane protein"
                     /protein_id="HNE02936.1"
                     /translation="MKLPTLLSYLVSLATPLVLIGLALRILLTPLYYTVEYNMPYFPA
                     DEYGFTKEDRLKWAKPSVEYLVNSADISYLGDLKFDNGTPIYGESELSHMHDVKNVVQ
                     GALKVWYLSIAVLILLAILAQRNGWMPLYLTGLRRGGWWMIGLAVTLGFIAGAGILLN
                     PDIFWNFFAWFHSLFFEGDSWLFYYSDTLIRLFPIRFWQDAVIAMAVIALGGGLGLAF
                     GLKTTN"
     gene            complement(4720..>6039)
                     /locus_tag="PLT08_00350"
     CDS             complement(4720..>6039)
                     /locus_tag="PLT08_00350"
                     /inference="COORDINATES: protein motif:HMM:NF037959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused MFS/spermidine synthase"
                     /protein_id="HNE02937.1"
                     /translation="TVGLVPYVAVPVLRSAASAFEALSVGIMAGSFIVVLILFIVPIT
                     LLGAISPYAIRLSVDDASKAGQISGQIYAVSTLGSFIGTFLPTLVFIPTVGTRLTFAI
                     FGMTLLVVALVGLARFASTRDMLKLIWMPILLAVIAFIFSSSALKTNKGQVYETESEY
                     NYIQVQESNGYTLLRLNDGQGIHSIYHPENLFYNGPWELFMVGPYFYENRSPDDIKEM
                     AIIGLAAGTAARQATIVYGEDLHIDGYELDPKIAEVGYEYFHMGSPNLNVILGDGRLN
                     LDRSTKKYDIIAVDAYRPPYIPPHMTTLEFFEICASHLADNGVLTLNVGSTPGDRRLI
                     DGLATTMAQVFPSIHIMDIPGTLNTMIYATKQPTTADAFLANTLRIAEDKSLNPLLVT
                     TMSSAYANLKPGYETTTVFTDDLAPIEWIVNDMVVKFVLEGGLEFLQ"
     assembly_gap    6040..6139
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<6140..6428)
                     /locus_tag="PLT08_00355"
     CDS             complement(<6140..6428)
                     /locus_tag="PLT08_00355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE02938.1"
                     /translation="MKRYLLFTVFISGMTTLAAELAAGRLIGNVFGTSNLVWASIIGL
                     ILIYLTLGYFLGGKWADKDPSPLAMYRILAWGAFTVGLVPYVAVPVLRSAAS"
     gene            complement(6519..8321)
                     /locus_tag="PLT08_00360"
     CDS             complement(6519..8321)
                     /locus_tag="PLT08_00360"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HNE02939.1"
                     /translation="MKTKSTNPLSLFFNLTVLAAYFHALMEWLFFVTQSSSLSTLSLL
                     DKIEVLFSTGGVIVLLLLALLVVFAVPALKWKFFGYVPAALMVSVTALVMIDNFTYTV
                     FKFGIVSTSGAWRLLYTVAFVLLFYWVFRFVERQKLWKGVSFLSLGLLGVSTVVILSI
                     YNSRSPYMSDFTPEATEASAEHPNILILGSDGLSASYLSAYGFEGNTTPFIAELTQTS
                     LVAENAFPNVSSTTGSTTSALTGKMPAEVQVFRYPDILSKEDSFEHLPGILKHMGYKT
                     VELGTTYYVDARKLNLADGFELVNNVSVDNPILDAIQPLLGNSPSAFFLQTVTERASD
                     RLFHIFFIKEMENPFLQVNNPNARMTDEERTQQIINLLETSDRPVFVFSHYMNTHGPL
                     FSTDDKHVEENEDSAESEKEWDVELYKKAIRTFDDHVREIYTYLEESGKLDNTIIVIY
                     TDHGYRYVVNQRIPIIFHFPNNDHAGTIQNNTQIIDIPATLLDYINVDQPEWMTGSSL
                     LQGEPPAMREIISTTAGSPKKIKPPFYQIKSLQFIVCQKWYSWNVQDNEFKTGEVKSH
                     TAKCEPNLLPSDDEIRQRMIEYFKKYNYDTSSLE"
     gene            complement(8362..8976)
                     /locus_tag="PLT08_00365"
     CDS             complement(8362..8976)
                     /locus_tag="PLT08_00365"
                     /inference="COORDINATES: protein motif:HMM:NF013382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HNE02940.1"
                     /translation="MRFFFNLLYHPFAFTYDLVAWVVSFGKWNDWVRSISPHLEGTRL
                     LELGHGPGHLQRFLLDLGLNPVALDESTQMGQIAKRRLGSKQKLTRGLAQNLPFAKAS
                     FETVISTFPSEYIFDPKTLAEIKRVLVNEGKLIVLPAAFPSNGFLKWLYKVTGESPEA
                     LDDALKERLKSPFVSAGFKVEIQMIEMKSSTLVLILARSILHSS"
     gene            complement(9046..9426)
                     /locus_tag="PLT08_00370"
     CDS             complement(9046..9426)
                     /locus_tag="PLT08_00370"
                     /inference="COORDINATES: protein motif:HMM:NF024143.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HNE02941.1"
                     /translation="MSDRDEFGAFLVGFVVGGLTGAVVALLFAPQSGEETRALIKDKS
                     IELRDKAQVSAEEAYARAEAAAKEARARAEELAKEARTRAEELTKEARTRAEQLASEV
                     KERGKGAIEAVRKPKKDGSEATPI"
     gene            complement(9462..9926)
                     /locus_tag="PLT08_00375"
     CDS             complement(9462..9926)
                     /locus_tag="PLT08_00375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE02942.1"
                     /translation="MALPNPESTPQTPPPLTPEQLAELREQERKQKQIMAGIIAVVVL
                     LVALLGIAIYFLLQPTTPTDKIRDVFIIVVALETLVIGVALIVLVIQLASLINLLQNE
                     VRPILQATSETVNTLRGTAEFLGENFVEPVIKLNGYLAGLKRMLELLGVKPK"
     gene            complement(10049..>10235)
                     /locus_tag="PLT08_00380"
     CDS             complement(10049..>10235)
                     /locus_tag="PLT08_00380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE02943.1"
                     /translation="LVKPFDALMPDSYTSEGLRVISGVTYIGDNSKAKRELGYNPRPV
                     SEGWVETVKYEKQLSGR"
BASE COUNT         2719 a         2343 c         2543 g         2530 t
ORIGIN      
        1 agggtcaaac agagaaatgg tacaagcttg cccaacaagt gtcaaaacga tcgccgcgga
       61 tatccaacgg cgcagtggaa tgatcgaacc tttcatgttc ttctcctcaa ggcgcatcag
      121 tgcgcttctt tccaaaccct agcctagctc aacgtcactg gctgcttcct gaacttcggc
      181 gtttttgctt cgcccggcgt aggcgagata gcttaagccg atactgataa agtataacaa
      241 aatcatcggg accatcacca accccatatt gaccgggtct acggtaggag tgatggcagc
      301 cgcaagcacg gcaataataa cgattgccaa tctccattgg tcagcaagga atttcgggtt
      361 gaccaagcca atggcagtaa gtacataaat aagaagcgga aattcaaaga agccgccgat
      421 ccataacatc agcccggtga cgaatgaaaa atattctcgt gcagaccaaa attgcgagat
      481 ggatgtgaaa ctccctaaaa agggcaaggc agtcggtaat aagacgtaaa aagtaaacgc
      541 cataccaagt agaaaaagaa gggtggcaag cggaatgcca accagcccta acttcttttc
      601 gcgaggcttc aaaccgggtg cggcgaacag ccagaactca aacgcaatat aagggaaggc
      661 gatcgcaata ccaatggtca ttgccacacg catgaaaacg ccgatctctt ctgtcacttc
      721 aatggcttgc agcttttcaa gtccgccaac aggttcagag agaaatgcca tggcaggttc
      781 tgccacccag aatgccgcga tcactaaaat tgataaggca atgatcgagc gcaaaaggtg
      841 catgcgaaag gcttcgatct gttcccataa ctgatcgcgg acttcttttt ctgtgaccaa
      901 atccgcaaag acatccgcaa tgggacgttc ttcgggttcg ctattaagca gcttgaaaaa
      961 acgataaccc gcccggaagg ggaaggctat gatggcatag acaaggcgaa atggaagggt
     1021 gataacccac caaacaccct taaaaaactt acgcattatt cggagtttcg ctttcggggt
     1081 tgtttttctc ttgcgggttt ggaaggataa cattctcacc gccaggcttg atgggcgatg
     1141 tggcagaata tggctgaggt tgtggtcgag agtttgcggg ggcagaccgg gctgggtctg
     1201 gggcggaggc ggcgctcttt atctcgcgat tgaccttatc caaatcctca tttgcttcgc
     1261 ggatcaagcg atttggcaaa gtgcgcaagt cgcgcgaggt ttgttgaaaa agtttccagc
     1321 catcagatgt gatgatatct cgtaaaaact ttccgatggt cttgccggct ttttgcatat
     1381 ctttcggacc caaaataagc aaagcaataa tcacgacaaa aataagttca ggcattccaa
     1441 tgccaaggat ttccatgttt atgattccaa attggtttct tgaggtattt cgtttttcag
     1501 aacctgatag atttcctgct catgctcaac caaggtacca agcacaccat ttacaaagcg
     1561 ggcagtcgag tcagacccga acaacttggc taactctaca gcttcattga tcgcaacctt
     1621 taccggagtt tggcgcgaga ttgcaaattc ccaacatgcc atacgcaaaa tatttctatc
     1681 gatggcggca atttgatcga gtggccactc aggtgcatac tttgcgatca cgcggtcaag
     1741 gttttctcga atgggcaaaa taccaaaaat aatgctgcgg gcgaactctg ctaaatcttg
     1801 cggcagagtg gtttcttcta aacgagtttt caatacatca ccgggcaggt gtgtggaaag
     1861 atcagtttcg tacaatactt gcagggcgag cgcgcgcgcc cgggttcggg gcttcatttg
     1921 attatgcctc gtccccttca aaatcaatat cttcgatgtg gacattcact tcgcccactt
     1981 ccatgccaac catttcctga acggaacggg ctacattcgc ttgcacattg cgcccaactt
     2041 cgcggatgtt gactccatct ttgaggatga ggaaaaggtc aacgaagacc gtgttgtcct
     2101 tcgcttcaat gcggacgccg tcactggtgc cgcgccgaaa gagccgattc acgccgccac
     2161 tgaccggtgc cagtcggcta acgccgggga cttccaatgc ggcgagactg gcgatagtcg
     2221 tcaatacttc gggagagacg gtggtttttc cttgcgtaga ttcagtcatt attactcctg
     2281 ttttacatca ttgccatgcc gccgtccaca ccaatgacct gaccggtaat gtacgcggcg
     2341 ccgtcagagg cgagaaaggt agcagtaaag gcaatttctt cgggttttcc tttgcgccca
     2401 agcggcacaa gtttgagcgc agcttcaagg gcttcgggtg tcatggcatc gagaatctca
     2461 gtatctacaa atcccggcgc gatagcgttg acggtaatgt tacgagcaga gacttcgcgt
     2521 gccaatgctt tggtgaacgc gatctgacca cctttggagg cggaatagtt cgtctgcccg
     2581 gggttgccca tttgcccagc aaccgatgcc atgttgatga tgcgcccggt gcgtttgcgc
     2641 atcatgtgct taacggcagc ttttgagcaa ttgaaggtgc ttttgagatt ggtgttgata
     2701 acggcatccc agtctgcttc gggcatcatc ataatgagtt ggtcgcgtgt aatgccggca
     2761 ttattgacaa gaatatgcaa gtcgccaaaa gtatcaatgg caaacttaac caacgcctcg
     2821 gcttgcttaa aatcggagac atcagcttga aaggcggcgg cttttccacc cgcttcttga
     2881 attttcttca ccacttcttc tgccgactca ggggatttgt tgtaattcac caccaccgct
     2941 gcgccgcgat cagcgaattc gagagcgatg gcgcgtccaa ttccgcgtga accaccggta
     3001 acgatagcta ctttgttctc taatgataat ttgaaggaca taactgggct cctgtaaaga
     3061 aattatactt catctggaat tgagtaaaaa ttgaaatcaa ataggtgcct aaacccaaat
     3121 tgtgcataca aaccagctcc ggcagagctg gcggttaggg cgactatttt accctcacat
     3181 tgggctgctt ctgaaagaag ccgtgaaaga atggctttcc catagccttg tcgatgaaat
     3241 tcaggcaaag ttgccatcac ataaatgccg gggatacctt ttgcttctat gatggcacca
     3301 attgaaactg cttcaccttt tttcggtccg gcaaggaaca aacgagccga gctttcatta
     3361 gataaccaat cttgtgccat atcttcaaaa taagtcaacg cttcgccttc tgtaaactgg
     3421 aatgccctgc gccgaatggt acttgccatt tgcaaatctt tgatagatgc cgcacgccac
     3481 acctgtatgg atttgtccac ttgtggaaat gatgaaacat cttgttttgt tggtgcgatc
     3541 atggctggga gcggcggggc atcatataca cagccaaatt tttctagaac catcgcaatg
     3601 tttacagggc gcggctcggc attcatccac caataccaag ggacatttct ttctgcgaag
     3661 aagtcgatga cttgttttac ttccgcttct atttcagatg aagccgtctt gtctggaaag
     3721 ttggcaccaa gcgccagatt taagagcggc agttttaacc ctgtggaaga ggctctgact
     3781 cccatgccct gaaacgtttt tgccattggg ctgcgtgaag aaagccaagc ttggacgttt
     3841 aacgtatgtg catctgccat acggatgagg tctatttgcg aagacccaaa tagctcaaga
     3901 cattggtcat tggtaagagg cattttttct ccgtttgacg agatgatgca ttgcctagat
     3961 ttataaaaaa ataaccccga actgatgaca acgatttcag agaaatgtca ctcagatcag
     4021 ggttaacata taaatttaag aaggtttagt ttgttgtttt tagcccaaat gctaaaccca
     4081 acccgcctcc caaggcgatc actgccatgg caataactgc atcctgccag aagcggatgg
     4141 ggaagagacg aatgagggta tctgaataat agaagagcca agagtcgccc tcaaagaata
     4201 gtgagtgaaa ccaggcaaag aaattccaga agatatcggg gttgagcaaa atgcctgccc
     4261 cagcaatgaa accaagcgtg accgccagcc cgatcatcca ccaaccgccg cggcgtaaac
     4321 cagtgagata caaaggcatc catccgttgc gttgggcgag gatggcaagc agaataagaa
     4381 cggcaatgga gagataccaa accttcaaag cgccttgcac tacattcttg acatcatgca
     4441 tgtgactaag ctcagattcg ccataaatag gggtaccgtt atcgaatttt agatcaccaa
     4501 gataagaaat atcagcgctg ttgacgaggt attccaccga aggttttgcc catttcaaac
     4561 ggtcttcctt cgtgaagccg tattcatcgg cagggaagta gggcatattg tattcaaccg
     4621 tgtaataaag cggcgttagt aaaatgcgca atgccaaccc aatcagaacc agtggcgtgg
     4681 caagagagac aagataagag agaagagttg ggagtttcat tattgtaaaa attccaatcc
     4741 gccttcgagc acgaatttca caaccatgtc gttgacgatc cattcaatgg gggcgagatc
     4801 gtcggtgaag acagttgtgg tttcgtagcc gggtttcaag tttgcataag cagatgacat
     4861 ggtcgtcaca agtaatggat ttaagctttt atcttctgca atgcgcaatg tgtttgcgag
     4921 gaatgcatct gctgttgtgg gctgtttagt ggcatagatc atggtgttga gcgtgccggg
     4981 aatatccatg atgtgaatgg atgggaaaac ttgagccatg gtggtggcga gtccatcgat
     5041 aaggcggcga tctcccggtg tggatccaac gttgagggtc agcactccgt tatcggctag
     5101 gtgagaggcg cagatctcaa agaattcaag cgtcgtcatg tgtggaggga tgtagggcgg
     5161 gcggtacgca tccacggcga tgatgtcata tttttttgtg gagcggtcaa gattcaatcg
     5221 cccgtctccg agaataacat ttaggtttgg cgaacccatg tgaaagtatt cgtagccaac
     5281 ttcagcgatc tttgggtcga gttcgtagcc gtcaatgtgc aggtcttcgc catagacaat
     5341 cgtagcttga cgagccgccg tccctgctgc aagcccaatg atcgccattt ctttgatgtc
     5401 atctggcgaa cgattttcat agaagtatgg tcccaccatg aagagttccc atggaccgtt
     5461 gtagaaaaga ttctcaggat gatagatgga gtgaatgccc tgaccgtcgt tgaggcgcaa
     5521 taaagtgtat ccgttcgact cttgaacttg aatatagttg tattcagatt cggtttcgta
     5581 cacctgccct ttattggttt tcaatgcaga acttgaaaag atgaatgcaa tcactgccag
     5641 caaaataggc atccaaatta atttgagcat gtcgcgagtg ctggcaaagc gagccagacc
     5701 caccagtgca accactaaaa gcgtcatacc gaaaatggca aaggtgaggc gtgtgccaac
     5761 ggtgggaata aaaacaaggg tgggcaagaa ggttccaatg aacgaaccca acgtagacac
     5821 agcatagatc tgcccggaaa tttgaccagc tttgctggca tcatctaccg agagacgaat
     5881 ggcatatggt gaaattgctc caagcaaggt gatgggcaca ataaatagga tgaggacaac
     5941 gatgaatgac cctgccatga tgccgacact caacgcctca aacgcgctcg ccgcggacct
     6001 gagaacgggt acggcgacat aaggcacaag ccccactgtn nnnnnnnnnn nnnnnnnnnn
     6061 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6121 nnnnnnnnnn nnnnnnnnnc gctcgccgcg gacctgagaa cgggtacggc gacataaggc
     6181 acaagcccca ctgtaaacgc tccccacgct agaatgcgat acattgccag tgggcttggg
     6241 tctttatctg cccatttgcc gccaaggaag tatcccaacg ttaggtagat gaggatcaag
     6301 ccgatgatgc ttgcccaaac cagattgctg gtaccaaaca cattaccaat gaggcgcccc
     6361 gccgcaagtt cggctgcaag cgtggtcatg ccagagatga aaaccgtaaa gagtaggtat
     6421 cgtttcatga aaatccctat aagaagatgc gcggattata gcgtacttca tgcagtgaga
     6481 aataagaact cccttagcgc ctaagggagt tctggatgtt actcaagcga actggtatcg
     6541 tagttatatt tcttgaaata ttcgatcatt ctttgacgaa tttcgtcatc agatggcaag
     6601 agattcggct cgcattttgc ggtgtgactt tttacttcgc cagttttgaa ttcattgtct
     6661 tgcacattcc atgaatacca tttctgacag acaatgaatt gcagactttt gatctgatag
     6721 aacggtggtt tgattttttt gggtgaacct gctgtggtac tgatgatctc tcgcatggcg
     6781 ggcggttctc cctgcaagag cgatgagccg gtcatccact cgggctggtc aacgttgata
     6841 tagtccagca aggtagcggg gatgtcaatg atctgcgtgt tgttttggat cgtgccggca
     6901 tggtcattgt ttgggaagtg gaaaatgatt gggatgcgct ggttgaccac atagcgataa
     6961 ccgtggtctg tgtagatgac gatgatggta ttgtctaact tgccagactc ttccaaatac
     7021 gtatatatct cgcggacgtg gtcatcaaag gtgcgaatgg cttttttgta aagttctaca
     7081 tcccattctt tttctgactc agcgctatct tcattttctt ctacatgttt gtcatccgtc
     7141 gaaaagagcg gaccgtgtgt attcatgtaa tgagagaaga cgaagaccgg gcggtctgag
     7201 gtttccagca agttgatgat ctgttgtgtg cgttcttcat cggtcatacg ggcattgggg
     7261 ttgttgactt gcaggaaggg attttccatt tccttgatga agaaaatatg aaacaaacgg
     7321 tcgctggcgc gttctgttac ggtttgaaga aagaaggcag aaggagaatt tcccaataac
     7381 ggttgaatgg catccaagat ggggttatca acagagacgt tgtttactaa ttcaaagcca
     7441 tcagccaagt ttagctttct cgcatctaca taataagtag taccgagttc cacggttttg
     7501 taacccatgt gctttagaat gccgggcaaa tgctcgaagg aatcttcttt ggagaggata
     7561 tcgggatagc gaaagacctg aacttctgca ggcattttcc cggtcaatgc agaggtggtg
     7621 gagccggtgg tgctggaaac gttagggaag gcattttcag ctacgagcga tgtttgggtc
     7681 agttcggcga tgaagggtgt tgtattgccc tcaaacccgt atgcggatag atagctggca
     7741 ctcaaaccat cactgcccag gattaggata ttggggtgtt ctgccgaggc ttctgtcgct
     7801 tcgggagtaa agtcgctcat gtagggagag cgcgaattgt atatggagag aatgaccact
     7861 gttgaaacgc cgagcaaccc caaagaaagg aaagaaacac ctttccaaag cttttgtctc
     7921 tccacaaagc ggaagaccca ataaaagagc agcacaaagg caactgtata caacaagcgc
     7981 catgctccag atgtggagac aatcccaaat ttaaaaacgg tataggtgaa gttatcgatc
     8041 atcaccaaag cagtgacaga taccatcaag gcggctggaa catacccgaa aaacttccac
     8101 ttaagagcgg gcacagcaaa aacaaccagc aacgccaata aaagcagaac gatcacaccg
     8161 ccggtcgaaa acaagacctc gatcttgtcg agaagggaaa gcgtggatag agacgaagac
     8221 tgcgtaacaa aaaacagcca ttccataagg gcgtgaaagt atgcggcgag gacggtcaga
     8281 ttgaaaaata gagataaggg atttgtggat tttgttttca taaagtcggc atattttaca
     8341 cccctgtatt aaaaaaaggc tttaggaact atgaagaatg gatctagcca gaattagtac
     8401 caacgtgctc gatttcattt ctatcatttg tatttcaact ttgaacccag cgctcacgaa
     8461 cggacttttt agcctttctt tcaaggcgtc gtctaaggct tctgggcttt cgccagtgac
     8521 cttatacaac catttcagga atccattcga tgggaaggcg gcaggtagaa cgatcaattt
     8581 cccttcattt acaagaaccc gctttatttc tgccagtgtt ttggggtcga agatgtattc
     8641 acttgggaag gtggagataa cggtttcaaa cgaagccttt gcaaacggca agttttgagc
     8701 gagcccgcgc gtcagttttt gcttggagcc gagacggcgt ttggcgattt gccccatctg
     8761 tgtagattca tctagggcaa caggattcag ccccaggtcc agtaagaagc gctgcaagtg
     8821 accgggacca tgccccaact caagaaggcg agtcccttca aggtgtgggg aaatgctacg
     8881 aacccagtca ttccacttcc caaacgaaac gacccacgcc accaggtcat atgtgaaagc
     8941 gaagggatgg taaagcaggt taaagaaaaa acgcatgaag tgacgcatag cagaaataaa
     9001 acgagggttt gacctgagtc aaaccctcat ctgtttcaat aaagattaaa taggggtggc
     9061 ttcgctgccg tctttcttcg gcttgcgaac ggcttcgatg gcgcccttgc cgcgttcttt
     9121 tacttcagaa gcgagctgtt ctgcacgagt gcgagcttct ttggtgagtt cttcggcgcg
     9181 ggtgcgagct tccttggcaa gttcttcagc gcgagcgcgg gcttctttgg cggcggcttc
     9241 tgcgcgggca taggcttctt cagcgctcac ttgggcttta tcgcgcaatt caatcgattt
     9301 gtccttgatc aatgcacgag tttcttcgcc agattgagga gcaaagagca aagcaacaac
     9361 agcgccggtg agcccgccga caacaaaacc tacgagaaag gctccaaatt catcacggtc
     9421 agacatggta gttctccttt ttagtcagat gatgtcatat actatttagg ttttacacct
     9481 aacaattcta acatacgctt taagcctgcc aaatagccat taagtttgat cactggctca
     9541 acaaaatttt ctcccaaaaa ttctgcggtg ccgcgcaggg tgttcacggt ttcgctggtt
     9601 gcctgcaaaa tggggcgcac ttcattctga agcaggttga tgaggctcgc caattggatg
     9661 acaagcacga tcaacgccac accaatcacc aaggtttcaa gcgccacgac aataatgaat
     9721 acatcgcgga ttttgtcggt gggggtggtg ggctgcaaca ggaaataaat tgcaatgcca
     9781 agcaaagcaa ctaacaaaac gaccacggca ataatgcccg ccatgatctg tttttgcttg
     9841 cgttcctgtt cgcgcagttc agccaattgt tcaggagtta acggaggggg agtttgtgga
     9901 gtggattcag gattgggcag tgccatgtct gtattatagc agattagata tacgaaaaaa
     9961 agccagagga tgaaacaagt cgcctctttc ccgtgccgcg cttgtacctg tccatctggc
    10021 ttttttcttc tgccaaactt ggttctgcct agcgtcccga taattgtttt tcgtacttaa
    10081 ctgtttctac ccaaccctca ctcaccgggc gcggattgta gcccagttcg cgtttggctt
    10141 tgctgttatc accaatgtac gtcacgcccg atatgactcg caacccttcc gatgtgtatg
    10201 aatcgggcat cagcgcatca aaaggcttga ccaaa
//
LOCUS       DAOQJE010000008        10052 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_3514_length_10052_cov_6.178354, whole genome shotgun sequence.
ACCESSION   DAOQJE010000008 DAOQJE010000000
VERSION     DAOQJE010000008.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 10052)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10052)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10052
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3514_length_10052_cov_6.178354"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1430)
                     /locus_tag="PLT08_00385"
     CDS             complement(<1..>1430)
                     /locus_tag="PLT08_00385"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HNE02944.1"
                     /translation="HNRYRIVDILGQGGMGSVYRAIDENLGVAVALKENLFTTDEYAR
                     QFRLEAVILANLRHPNLPRVSDHFVLGDLGQYLVMDFIEGEDLRYRMERLGMLEEEDA
                     VHIGAAMCDALAYLHTRKPPILHRDIKPGNVKISPDGHIYLVDFGLAKVYQSASQATT
                     TGARAMTPGYSPPEQYGTARTDPRTDIYSLGATLYAALTGVIPEDGLARAMDNAQLTP
                     LRKRNSKVSRKLAASIEKAMAVDPSDRFQTAEEFKAGLLSSKSMTQQAPGSYKVIPPP
                     PDAVAASSHTHEVTVVDEALPVDGKKDRKALVGPVPAAGSNAHEDQPFVSPLKRQKER
                     ERKRRMALWRFALFAGILLLVLGSVVFFVPGVLPADVRAMVPFIGATATPTFTLTPVP
                     TQTLPATFTATLEPTSTPTLRPTNTPTATFTPTPLPGETITITPVASSGGTEVFMGGG
                     AGQIAFASDRTGSPQIFLVDLRGESA"
     assembly_gap    1431..1530
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(1702..2697)
                     /locus_tag="PLT08_00390"
     CDS             complement(1702..2697)
                     /locus_tag="PLT08_00390"
                     /inference="COORDINATES: protein motif:HMM:NF013718.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HNE02945.1"
                     /translation="MQTLIDFGVSFIIALQRLGEWQIPIMAFFSRLGTEEFFFIVLPL
                     LFWNIDSALGIRVAFILAVSNFINLIGKVGFAGPRPYWVSSHIQPWWPETSFGIPSGH
                     AQHAMSVWGIIALYKKEKWITIVCVILIFMIGFSRLVLGAHFPHDVILGWLLGAFLLW
                     LYVRYWDATVTWLSNMNFSKQVGVAFLVSMIFVVIGMIVTNTRSDFQIPESWMTNALR
                     SGIAPDPIDPNGVFTAVGTFFGLAVGAAWTHLRGGYQADGPVWKRVLRYVIGLAGVLI
                     LWQGLGVIFPRGDGLVVYSLRFVRYSLVGWWISGGALWVFKHFNLTTPSSRPTSI"
     gene            2769..5132
                     /locus_tag="PLT08_00395"
     CDS             2769..5132
                     /locus_tag="PLT08_00395"
                     /inference="COORDINATES: protein motif:HMM:NF023418.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminaseTgpA domain-containing protein"
                     /protein_id="HNE02946.1"
                     /translation="MEKTSVRNWDWISNLIMLFAVLFSAWRLQTADWTEGLGQVRNVA
                     VLGFALGLGVGYSQFKRKPIFFLVAGYTVAILIWQLLGTIPFDKDQTYLLDKFSILFG
                     RIFTSLGELFAGRAVEDQFFVVALLCIPYWFASLYSGFQLTRRSNFLGTILPNGVLML
                     IIHLYHYTNQDYTWMLGVYLFLALLLLSRLKYLADRRKWSKERIQVQSDSGLDITNTT
                     VAVATVLILLAWGSPYILPATTEGKQIWREVYGQIFPKDRFKNVFASVEKENQPKARN
                     FRPELALGTQTPQGNSIVFQVYVPLEAKKFPRLYWRGQVYDRYENGRWLTTSDAEVRQ
                     NPATGDITIPDSADRQRLSFTFDVMTSGQTTVYLPPQPIWINHSTILLSSDISNKTGE
                     DAILDIMAVRASPPFQDGDLYRVSSLLADPTAEELRLSGDAYPDWVKEKYLQLPENFS
                     PRIQALAQQITAPYNTPYDKTVAVTNYLRREIEYTGVIYLPNEDVDPLEYVLFDAKRA
                     FCNYYATAQVLMLRSVGIPARLAVGYAQGQPNIQKSLYAVREKDLHAWPEVYFPEYGW
                     IEFEPTTNQQPIERPEAREDLAQIAPFANNSNQPLFKPDVEFTPDQEPNVPDGGSASA
                     RSWLDWLASLLPWLGGVFFILLGIVLKRRFAPQVTVASVMKRAIDRSGWNAPRWLRRW
                     LAFATQPAIQRHFHNINISLRLLKRPQPVHVTAAERAQTLKGLLPSASASIDSLLNEL
                     QAELFTPNGGNEAIARRAAWDILSKTVQSKLKIIILGYNYEGIKETS"
     gene            5139..6851
                     /locus_tag="PLT08_00400"
     CDS             5139..6851
                     /locus_tag="PLT08_00400"
                     /inference="COORDINATES: protein motif:HMM:NF012437.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HNE02947.1"
                     /translation="MIENSTRPRTVHDQLSALAVLAGKIRAELGNAKLKLPKESLEQL
                     HYLQSTLETVGNKMEVFQREHGNMLALVETGQVINSSLELDEVLRIVMDNIVRLTKAE
                     RGFLMLRNEGGEMINRVARNWEMESINPSEKNVSRSVVQRVIDTGESVVTTNAQEDQR
                     FVGQESIVAFNLRSILCVPLKVKNDLIGVIYADNRIRAGIFAEAEKDLLEAFANQAAI
                     AIDNARLFSSLKGTLEEVTALKNLMDNVFASIASGVITADVQDTITLANRAAETILGS
                     PTQDIIGHHLKDALASVSGELAPHLMEVRKTDKPIVDLEISHNLPKRGNVDWRLNLSP
                     LKDAGQKTQGVAIVLDDLTERKKLEAQRRLFERMVSPAVIEQLDPNGLQLGGKRTEIT
                     ALFADIRGFTTYSEHLAPERLVSILNLYLAAMADAVLAQEGTVDKFMGDAIMAWFNAP
                     IPQPDHTLRAVKTALAIRESIENLYKELSSDSHLAFGVGIHFGDAVLGLIGTEKRLEY
                     TAISDTVNTAKRLQENSARNQIIISQDAYERVKNDVNVKPISEITVKGRSKPIEVFEV
                     LGLK"
     gene            complement(6900..7988)
                     /locus_tag="PLT08_00405"
     CDS             complement(6900..7988)
                     /locus_tag="PLT08_00405"
                     /inference="COORDINATES: protein motif:HMM:NF013696.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HNE02948.1"
                     /translation="MTLAKKVLDLAIQIQQIASPTFAEGPRGEFIRDLFIQEKLDDVS
                     MDSLGNVYARLPGKQKKARALIVSAHLDTVFPAGTKLQIKEEAGKVFGPGIGDNSIGV
                     AALFGILWGIRERKIELKHDVWFVANVGEEGLGDLRGMRGVVERFGNDELGYLVLEGL
                     ALGHVYHRAIGVRRYRITAKTRGGHSWSDYGQPSAVHELAAMVTQLTQVRLPREPRTT
                     MNVGTIHGGTGVNVLAAEAKCELDLRSEDPVVLAKIVSQVEDIILHWNHEGVKIQAEI
                     IGERPAGQVASDHPLVKLAVESLNAQGMEAILTTGSTDANVPLSKGIPALVMGITTGG
                     GAHTMGEYIDTEPIERGMESLIRFVELS"
     gene            complement(8031..9041)
                     /locus_tag="PLT08_00410"
     CDS             complement(8031..9041)
                     /locus_tag="PLT08_00410"
                     /inference="COORDINATES: protein motif:HMM:NF012515.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase family protein"
                     /protein_id="HNE02949.1"
                     /translation="MTSNFALAGKLTREYLLPLNGSPRLDSPGGNLLYAMSGLAVWDQ
                     SIALIARVNREYPQEWISDFQTRGLDVDGIYVDPESDRVDIRSFIAYTDTNERSITNA
                     VSHFARREMTFPKSLLGYHPQDPTIKDLRETDPLSPAARFVPKEYRNIGFVHLCPFDF
                     TSQSQLVNLFKGGSNQMVSLDPAPGYMKPKFWRELRVVLQGVTAFLPSEDELRSLFWG
                     ESHDLWEMAQRISEHGPQIIVIKRGPQGQLVYDAASKRRYEVPAYTARIMDPTGVGDA
                     FCGGFLAGYQRTNDPLQAALHGNVSASLKIEGVGVYATLDAMPGLADARLHALKEMAR
                     EI"
     gene            complement(9047..9610)
                     /locus_tag="PLT08_00415"
     CDS             complement(9047..9610)
                     /locus_tag="PLT08_00415"
                     /inference="COORDINATES: protein motif:HMM:NF015319.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HNE02950.1"
                     /translation="MTTYCDYCNSHPEDTFNKTYHDTQYGFPIDDDNELFERLVLEIN
                     QAGLSWILILKRAQNFRKAFHKFNIKKVAKYTEADRERLLNDEGIIRNKLKVNAAIVN
                     AQKILELQKEYGSFKGWLDAHHPLTKDEWTKLFKKTFVFTGGEIVNEFLMSTGYLPGA
                     HQKDCPIFKKAAAKKPAWMQPSKARKS"
     gene            9677..>10052
                     /locus_tag="PLT08_00420"
     CDS             9677..>10052
                     /locus_tag="PLT08_00420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE02951.1"
                     /translation="MPIPVVTTKLRIPPLRTKVVIRQRLLERLKEGLSRTLTLVSAPA
                     GFGKTTLLSEWLGEVNLKIAWLSLDDGDQDITRFITYLISALQIIDPSIGESVLGALK
                     SHQPPAVDTLLIALLNDIDSVSQ"
BASE COUNT         2741 a         2396 c         2273 g         2542 t
ORIGIN      
        1 ggcactttca ccacgtaagt ccactaaaaa tatctgagga gaacccgttc ggtcagaagc
       61 aaaagcaatt tgccctgctc cccctcccat aaatacttct gtccctcctg aggaagcaac
      121 tggcgtaatt gtaattgttt cgcctggtaa tggtgttggg gtaaaagtag cagtaggggt
      181 gttagtcggt ctcagtgtag gggtagaggt gggttccaat gtagccgtaa aagttgctgg
      241 caacgtctgt gttgggacag gtgtaagcgt aaaagttgga gtcgctgttg ctccaataaa
      301 aggtaccatt gcacgaacat ctgccggcaa aacacctgga acaaaaaaga caacactacc
      361 tagaactaac aacaaaatac ctgcaaaaag tgcaaaacgc cataaagcca tacgacgttt
      421 acgttctcgt tccttttgtc tctttaaagg agatacaaaa ggctggtctt catgtgcatt
      481 tgacccagca gcagggacgg gtcctactaa agcctttcgg tcttttttac catctacagg
      541 taaagcttcg tcaaccacag ttacttcatg ggtgtgagat gaagcagcaa cggcatctgg
      601 cggaggtgga attaccttgt aagagccagg tgcctgttgc gtcattgact tagatgagag
      661 caacccagct ttgaattctt cagccgtttg gaaacggtcg ctagggtcaa ctgccatcgc
      721 tttttcaata gaagcagcta attttcgtga aacctttgaa tttctcttgc gcaatggagt
      781 taactgtgca ttatccattg cacgcgccaa accatcttct ggaatcacac cggtcaatgc
      841 agcgtataga gttgcaccca aagagtaaat atcggtacgc gggtcagtac gggcagtgcc
      901 atactgttct ggaggagaat agccgggagt catggcacga gcacccgttg tggtagcttg
      961 gctcgcgctt tgatatacct ttgccaaccc aaaatccact agataaatat gaccatctgg
     1021 tgaaattttg acattgcccg gtttgatatc acgatgaaga atgggcggtt tacgagtgtg
     1081 cagatacgcc aaggcgtcac acatggctgc gccaatatga acagcatctt cttcctctaa
     1141 catacctaac cgttccatac gatagcgaag atcttcgccc tcgatgaagt ccatgaccaa
     1201 gtattgcccc aaatcaccta aaacaaaatg gtcagaaacg cgtggcaagt tgggatgacg
     1261 caaatttgcc aagattacgg cttcaagacg gaactgacga gcatactcat ctgtagtaaa
     1321 cagattttct ttgagtgcta cagccacacc aagattttca tctatggcgc gatatacaga
     1381 gcccatcccg ccttgcccaa ggatgtctac gattcgatac cgattatgta nnnnnnnnnn
     1441 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1501 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn acagagccca tcccgccttg cccaaggatg
     1561 tctacgattc gataccgatt atgtatgaga gtgcctttat ccagcgtcat tgaaatccat
     1621 tcacctgcaa tatataaatt tccagcaaag actgtagatt taaaaaaatg ttactacttt
     1681 gatttggaca cgcgcattat atcagatgga ggttgggcga gaagatggtg tagttaaatt
     1741 gaaatgtttg aaaacccaga gggctccacc agaaatccac caccctacca aggagtaacg
     1801 gacaaagcga agagaataca ccaccaaacc atcaccacga ggaaaaataa caccaagccc
     1861 ttgccacaaa atgagaactc cagccaaacc gatgacatag cgaagtacac gcttccaaac
     1921 cggaccgtct gcctgatagc cacctcgaag gtgagtccac gctgcaccta ctgccagccc
     1981 gaagaaggtc ccaactgcag tgaagactcc gttcgggtcg ataggatcag gcgcgatacc
     2041 ggagcgaagc gcgttcgtca tccatgattc tgggatttgg aaatcacttc tcgtatttgt
     2101 aacaatcatc ccaataacca caaaaatcat agaaactaaa aaagcaactc ccacttgctt
     2161 tgaaaagttc atattggaaa gccaggtaac ggttgcatcc caatagcgga catatagcca
     2221 caacaaaaag gcgcctaaca accaaccaag gatgacatca tgcggaaaat gtgcgccaag
     2281 aactaaacgc gaaaagccga tcatgaaaat aagaatgaca cagacaatcg tgatccattt
     2341 ttcttttttg tacaaagcaa tgatgcccca aacgctcatg gcatgttgtg catgccctga
     2401 agggatgccg aacgaggttt cgggccacca aggttgaatg tgtgagctga cccaatatgg
     2461 gcgcggacca gcaaagccaa ctttgccaat aagattaata aaattgctca cagccaaaat
     2521 aaaagccact cgaatgccaa gtgctgagtc aatgttccaa aaaaggagtg gcaaaacaat
     2581 aaagaaaaat tcttctgtgc ctaatcggct gaagaaagcc atgatgggaa tttgccattc
     2641 gcccaaccgt tgaagtgcaa ttatgaaaga cacaccaaag tcaataaggg tctgcatcta
     2701 gtcctccagt agggtttttc ttaggaagtg aattgacgta acaatgcgac ccattgtaca
     2761 ctcaaagaat ggaaaagaca tcggtgcgta attgggattg gatatccaat ctgattatgc
     2821 tttttgcagt gttattttct gcctggcgct tacaaaccgc agactggact gaaggcttgg
     2881 ggcaagttcg caatgtggcg gtacttggtt ttgcgcttgg gctcggggta ggatacagcc
     2941 aatttaagcg aaagcctata ttttttctgg ttgcagggta tacagttgct attttgatat
     3001 ggcaattatt ggggacaatt ccatttgata aagaccaaac ttatttatta gataaattct
     3061 cgatcttgtt tggaagaata tttacatctc ttggtgaact ttttgctggt agagctgtgg
     3121 aagatcagtt ctttgtcgta gctttattat gcatccctta ttggtttgct agtctttaca
     3181 gtgggtttca gttaactagg cgttctaact ttttgggcac catccttcca aatggtgtgt
     3241 tgatgctgat catccatctc tatcactata ccaaccaaga ttatacttgg atgcttgggg
     3301 tttatctttt ccttgcatta ttactactca gtcggttgaa ataccttgca gaccgtagaa
     3361 aatggtcaaa ggaaagaatt caggttcaaa gtgatagtgg gcttgatatc acaaatacaa
     3421 ccgtggctgt tgccaccgtc ttgattttat tggcatgggg gtcgccatat attctgccag
     3481 cgacaacaga aggaaagcaa atttggcgcg aagtgtacgg gcagatcttt ccgaaagatc
     3541 gcttcaaaaa tgtatttgct tctgtggaaa aagaaaacca gcctaaagca cggaacttcc
     3601 gcccagagtt agcccttggc acacaaaccc cacaaggcaa ttcgattgtc tttcaggtgt
     3661 atgttcccct cgaagcaaag aaatttccgc gcctctactg gcgcggacaa gtgtatgacc
     3721 gttatgaaaa cggacgttgg ctaacaacca gtgatgcaga agttcgtcag aaccctgcca
     3781 ctggtgatat tactatccct gatagcgcag accgtcagcg gctttctttc acctttgacg
     3841 tgatgacctc tgggcagacg actgtttatc ttccaccgca gcctatatgg attaatcaca
     3901 gcacgattct gctttcttcg gatatttcga ataaaactgg agaagatgcc attcttgaca
     3961 ttatggcagt tcgcgcctct cccccatttc aagatgggga cttgtaccgc gtcagttcct
     4021 tgcttgcaga cccaacagca gaagaactgc gtttaagtgg agatgcatat ccggattggg
     4081 tcaaagagaa atatcttcaa ctgcctgaaa acttctcgcc acgcattcag gcactcgctc
     4141 agcaaatcac agctccctat aacaccccct acgacaaaac agtcgctgtt acaaactatc
     4201 tgcgcaggga aattgaatac actggtgtga tttaccttcc caatgaagat gttgacccgc
     4261 ttgagtatgt attgtttgat gctaagagag ctttctgcaa ttactatgcc acggctcagg
     4321 ttctcatgct acgcagtgtt gggatccctg cccgtttggc ggtgggatat gcccaagggc
     4381 agccaaatat tcaaaaaagt ttgtatgcgg ttcgtgagaa agacctgcat gcctggcccg
     4441 aagtctattt ccctgagtat ggttggatcg aatttgaacc caccacaaat caacaaccga
     4501 tcgaaagacc agaagctcgc gaagatttag ctcaaattgc tccattcgca aacaactcaa
     4561 accagccact atttaaacca gatgtggaat tcacgccaga ccaagaacca aatgtgccag
     4621 acggagggtc agcttcagcg aggtcttggc tagattggtt agcatcgctc ttgccgtggt
     4681 tgggaggcgt gttctttatc ctccttggaa ttgttctcaa acgacgcttt gctccacagg
     4741 tgaccgttgc ttctgtgatg aaacgagcaa tagaccgtag cggctggaac gcgccacgtt
     4801 ggctgcgtcg ttggctagcg ttcgccaccc agcctgcgat ccaaaggcat tttcataata
     4861 tcaatatcag ccttcgatta ttgaaacgcc cacagcccgt gcatgtgact gcggcagaac
     4921 gggcacagac cctaaagggg ttgctgccca gtgcgtctgc atcaatcgac agccttttga
     4981 acgagcttca agctgagttg ttcaccccta acggtgggaa tgaggcaatt gcccgccgtg
     5041 cagcatggga tatcctttct aaaaccgtac aaagtaaact aaaaattatc atcttgggat
     5101 acaattacga gggtatcaag gaaacctcgt aacaccccat gattgagaac tccactcgtc
     5161 cccgcaccgt tcacgatcaa ctatctgccc ttgccgtgct tgccggtaag attcgcgcgg
     5221 aacttggcaa cgcgaaattg aagttgccga aggaatcgtt agaacagcta cattacttgc
     5281 agtccacttt ggaaacggtt ggcaacaaaa tggaagtttt ccagcgtgag cacggcaata
     5341 tgttggcgct tgtggaaacc ggtcaggtca ttaactcctc tctagagttg gatgaagtgc
     5401 tacgcatcgt aatggataat attgtgcgcc tcaccaaagc ggagcgcggt tttctcatgt
     5461 tgcgaaacga aggcggggaa atgatcaacc gcgtggctcg caactgggaa atggaatcca
     5521 ttaacccttc tgaaaaaaat gtcagccgct cggttgttca gcgtgtgatt gatacgggcg
     5581 agtcggttgt cactaccaat gcacaagaag atcaacgctt tgttgggcag gaaagtatcg
     5641 ttgccttcaa tctacgttcg attttgtgcg taccccttaa agtaaaaaat gacctcatcg
     5701 gcgttattta tgccgataac agaatccgtg cagggatttt cgcagaagct gaaaaagatt
     5761 tgttagaagc gtttgccaac caagctgcca ttgccattga caacgcacgc ttgttctcgt
     5821 ctctcaaagg tacgcttgaa gaagtcacgg ctttgaaaaa cctaatggac aacgtctttg
     5881 cttccattgc cagtggcgtc atcacagcag atgtgcaaga tacgatcact cttgccaacc
     5941 gagcagcaga aactatcctc ggctccccca ctcaagacat tattggtcat catcttaaag
     6001 atgcgcttgc ttcggtttca ggtgaattgg ctccgcattt gatggaagtt cgcaagaccg
     6061 acaaacctat cgttgacctt gaaatcagtc acaacctgcc caaacgcggg aatgtagact
     6121 ggcgcttgaa tctctctccg ctaaaagatg cgggacagaa aactcaaggc gtagcaatcg
     6181 ttttagacga cctcacagag cgaaagaagt tagaggcgca acgtcgcctg tttgaacgga
     6241 tggtctctcc agccgtcatc gaacaacttg acccgaacgg tttacaactc ggcggtaaac
     6301 gcactgaaat caccgctttg tttgcagata ttcgtgggtt cacgacctat agtgaacacc
     6361 tcgcaccaga acgtttggtg agcattctca atttatatct cgctgccatg gcagatgccg
     6421 ttctcgcaca agaaggcacc gtagacaagt tcatgggtga tgctattatg gcttggttca
     6481 atgcacccat cccacagcca gaccatacat tgcgtgcggt aaaaactgcg cttgccattc
     6541 gcgaatcgat tgagaatttg tataaagaac tctccagtga ctcacatctt gccttcggcg
     6601 taggcattca ctttggagat gcagttctgg gtttgattgg tacagaaaag cgccttgagt
     6661 acacagctat cagcgacaca gtcaacactg ccaaacgctt acaagaaaat tctgcccgca
     6721 accaaattat cattagtcaa gatgcgtacg agcgtgtaaa aaatgatgta aacgtaaaac
     6781 cgatctcaga gatcacggtt aagggacgtt caaaaccgat agaagttttt gaagtgctcg
     6841 ggttgaaata aaacaaaagg cttccaaatg gaagcctttt tgattcgaaa taatggcact
     6901 taagataatt ctacaaaccg aatcaaactt tccattcctc gctcaatcgg ttccgtatca
     6961 atatactcgc ccatcgtgtg tgccccaccg ccagttgtaa ttcccatcac aagggcggga
     7021 attcctttgc tcaatgggac attggcatct gtagagcctg tagtcagaat agcttccatt
     7081 ccttgtgcat ttaaactttc taccgctaac ttgaccaaag gatgatctga ggcaacttgt
     7141 cctgcaggtc gttctccaat aatttctgct tgaatcttca cgccttcatg attccaatgc
     7201 aaaataatat cttccacttg cgagacgatc ttcgccaata caaccgggtc ttcggagcgc
     7261 aaatctaatt cgcacttggc ttctgcagcc aacacattca cacctgtccc gccgtgaatg
     7321 gtgccaacgt tcattgtagt gcgtggttct cgcggtaaac gaacctgcgt taactgagtg
     7381 accatcgccg ccagttcatg gacagccgag ggttgaccat agtctgacca ggaatgtcca
     7441 ccgcgggtct ttgctgtgat gcgatagcgc ctcactccga tcgcacggtg atacacatgc
     7501 ccaagcgcca agccttccaa aacaagataa ccaagttcat cgttgccaaa gcgctcgacc
     7561 acgccacgca taccgcgcag gtcgccgagc ccctcctccc ccacattcgc cacaaaccac
     7621 acatcatgct tcagctcaat cttgcgttcg cggattcccc aaagaatgcc gaacaacgcc
     7681 gccaccccga tggaattatc cccaatgccg ggaccaaaaa ccttgcccgc ttcttcctta
     7741 atctgtaact tggttcccgc cgggaacacc gtgtctaagt gggcagatac gatcaacgcc
     7801 cgagcctttt tctgtttccc cggcaaccgt gcatagacgt tccccaacga gtccatggac
     7861 acatcgtcaa gtttctcttg tatgaacaag tcacgtataa actcgccacg cggaccttcc
     7921 gcaaaggtag gagatgcaat ttgttgaatt tgaatagcga ggtcgaggac ttttttggct
     7981 agtgtcatga tggctgcaat cgtaaatcgt cagtcacaaa tcgtcaattg ttagatttct
     8041 cttgccattt ctttcaaggc atgcaaacgg gcatctgcca aaccgggcat ggcatctagc
     8101 gtagcgtaaa cgccaacccc ttctattttt agtgaagcag agacatttcc atgcaaagcc
     8161 gcttgcaagg ggtcgttcgt gcgctggtat ccagcgagga atccgccaca gaaagcgtca
     8221 ccaactccgg tcgggtccat gatgcgggca gtataggctg gcacttcata acgtcttttc
     8281 gaggcggcat catatacaag ctgaccttga ggtccgcgct tgataacaat gatctgaggt
     8341 ccatgctcac tgatacgttg tgccatttcc caaaggtcat ggctttcacc ccaaaacagc
     8401 gaacgcaatt catcttcaga tggaaggaag gcagtgacgc cttgcaacac aacacgtagt
     8461 tctcgccaaa attttggttt catataaccg ggggcagggt ctaaactaac catctgattt
     8521 gagccgccct tgaaaagatt aaccaattgg ctctggctgg taaagtcgaa aggacaaaga
     8581 tgtacaaagc cgatgttgcg atattccttc ggaacaaatc gtgctgcagg agaaagtgga
     8641 tcggtctcgc gtaagtcttt tatagttggg tcttgagggt gatacccaag cagggatttg
     8701 ggaaaggtca tttcgcgacg agcaaaatgt gaaacggcat tggtaatgct gcgttcattg
     8761 gtatcggtat atgcaataaa actacggata tcaacacggt cgctttcagg gtctacataa
     8821 ataccatcaa catcgagacc tcgagtttga aaatcgctta tccattcttg cggatactct
     8881 cgattcactc tcgcaattaa tgcaatggat tgatcccata cagccaagcc gctcatggca
     8941 tataaaagat tcccgccggg tgaatccaaa cgcgggcttc cattcagcgg aagaaggtat
     9001 tcgcgagtaa gtttgcctgc taatgcgaag ttggatgtca ttcaggttat gattttctgg
     9061 ctttggaggg ttgcatccaa gctggctttt tggcggctgc ttttttgaag attgggcagt
     9121 ctttttgatg cgcgccgggc agatagcccg tggacataag aaactcgttg acgatctcac
     9181 cacctgtgaa aacaaaggtc ttcttaaata gcttggtcca ttcgtctttg gtgagtggat
     9241 ggtgagcatc taaccagcct ttaaaggaac catattcttt ttgcaattca agaatcttct
     9301 gcgcattgac gattgcagca ttgaccttca acttattacg aataatacct tcatcgttca
     9361 aaagcctttc gcggtctgcc tcagtgtatt tggcgacttt cttgatattg aatttatgaa
     9421 aggctttgcg aaagttctgt gctcgtttca ggatcaaaat ccatgaaagt ccagcctggt
     9481 tgatctcaag cacgagccgt tcgaagagtt cattatcatc atcaatagga aagccatatt
     9541 gagtatcgtg gtaggtttta ttgaacgtgt cttcagggtg agaattgcaa tagtcgcagt
     9601 aagtggtcat gagtatattt tactatctcg ctaaaccctt gcggagattc cagctaggca
     9661 ctttataatt acccacatgc caatccctgt tgtgacgacg aagttgcgca tccctcccct
     9721 gcggacaaag gttgtgatcc gccaacgctt gctggaacgg ttaaaggaag ggttgagccg
     9781 tacgctgacg ttggtttctg caccggcagg ttttggaaaa acaactttgc taagtgaatg
     9841 gttgggagaa gtaaatctaa aaattgcatg gctatctttg gacgatggcg accaagacat
     9901 aacacgtttc attacatatt tgatatcagc attacaaatc atagacccca gtattggcga
     9961 aagtgtactt ggcgcactta agtctcatca gccgcccgca gtagacacct tattgatagc
    10021 cttactcaat gacatagact cagtctcaca ag
//
LOCUS       DAOQJE010000009        10029 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_3532_length_10029_cov_5.821737, whole genome shotgun sequence.
ACCESSION   DAOQJE010000009 DAOQJE010000000
VERSION     DAOQJE010000009.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 10029)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10029)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10029
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3532_length_10029_cov_5.821737"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..830
                     /locus_tag="PLT08_00425"
     CDS             <1..830
                     /locus_tag="PLT08_00425"
                     /inference="COORDINATES: protein motif:HMM:NF027969.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HNE02952.1"
                     /translation="FGSALTNFGVRMFLDSFIQFAPPPQPYLSDTGLVLPEDTEFTGF
                     IFKIQANMNPKHRDSVAFLRICSGKFERGMDLLHAPSGKTLKLMRPYKAFANEREIIE
                     EAYPGDVLGLPNNGIFSIGDTLCTGKTIQFAPIPRFQPEHFALLRNVDVSKQKQFAKG
                     LQQLESEGAVQILYNVNAFKREPILAVVGQLQFDVVQARLESEYNVKTELERLNHSHL
                     RWVLGEDKDIEALANRGDAILARDSRNAWAMLFQTPFLLKYYSEKNPNLKFVEITEL"
     gene            899..1627
                     /locus_tag="PLT08_00430"
     CDS             899..1627
                     /locus_tag="PLT08_00430"
                     /inference="COORDINATES: protein motif:HMM:NF012792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNE02953.1"
                     /translation="MDKTSFERLIHESWATYFGCSVETTEQPGTTLLPESKYEGDKVI
                     ALWHIGKHSFVQLDPAYYSQLEDLIKKLPHHTSLTGEQIEAAWGKDAISSQDVGFMHY
                     LFPSDLPHYQPSLPFTLRQLTEADAGLMSTLQAVNSPEDVDEAFVEVTHEIAFGCFHG
                     EELVAAASGYERTGFLDLGVLTHPDYRKKGLGKAVVGATCNWANQKGIIAQYRHNALN
                     TSSQNVATSLNFQKYFSSESFFLR"
     gene            1632..2906
                     /locus_tag="PLT08_00435"
     CDS             1632..2906
                     /locus_tag="PLT08_00435"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNE02954.1"
                     /translation="MAYLKTLYNQYPRQFWLMILGIVISTAGGGMVWPFMLIYAGGKL
                     GLPLSAVASLISINAGVGLFASFLAGTLADRIGRKVVMVSSLVMMGVAYLLLIKAETY
                     LQFALLMGMVGLSNPLYQVGADAMLADMIPTQKRTDAYAINRIAYNASFGIGPAIGGF
                     LASTSYALAFYGASTGFFIYGIIWFFFAHETLDKTVREEKASTLAGQAQIQNEAARSD
                     GYIHVFKDKAYMAFVTLFAIGMIAPSILWILLPVYTKTNFNLPEALYGWIPTTNAFMC
                     VFLQYAVSNQTRKFKTLPVASVGMLVYALGAGSVALMTGFTGFWLSMVILTFGELILI
                     PIASKYVADIAPENLRGRYMSLYWFGWGMSRTLAPLIGGYLNDNISPRAIWIGGLLFG
                     LTSTLGLILLNSLTNTPTALQTRPKIDTTHGD"
     gene            2909..3295
                     /locus_tag="PLT08_00440"
     CDS             2909..3295
                     /locus_tag="PLT08_00440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HNE02955.1"
                     /translation="MKQTLALISYVVRDYDEAIAFFTNAMQFDLVEDTLQENKRWVVV
                     RPRGGVGVSLLLARADTPEQQAAVGNQTGGRVFLFLHTDNFKRDYEHMRLHSIEFMEE
                     PRHEAYGTVAVFKDLYGNKWDLLQPK"
     gene            complement(3304..3903)
                     /locus_tag="PLT08_00445"
     CDS             complement(3304..3903)
                     /locus_tag="PLT08_00445"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HNE02956.1"
                     /translation="MTNWAQRVTLQGKYVRLEPMGEQHISGLAEIGAGQDFWSFMLYG
                     DMKTEADMRNWVLDILEREKKGGDLPFVVIHLASGKVAGATRYLNIIPKDRGLEVGGT
                     WYGKEFQRTAVNTECKYLLLTHAFEVLKTIRVQIKTDSINLRSQAAIERIGAVKEGVL
                     RNHMILPDGRIRHSVFYSILDSEWVGVKKNLEAMLKKNE"
     gene            complement(3900..4361)
                     /locus_tag="PLT08_00450"
     CDS             complement(3900..4361)
                     /locus_tag="PLT08_00450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HNE02957.1"
                     /translation="MIHYLEREQVIPGTMKDIWEFFCNPYNLNTITPPDMNFKIIKGG
                     DRKMYPGQMIEYRVEFIRGIRSLWLTEITHVREGEFFVDEQRIGPYRLWHHEHIFESM
                     NGGVKMTDRVTYMLPFELLGDIVHAVWVRRRLDSIFDFRTQKTTELFGNLK"
     gene            complement(4358..5929)
                     /locus_tag="PLT08_00455"
     CDS             complement(4358..5929)
                     /locus_tag="PLT08_00455"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: protein motif:HMM:NF001126.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HNE02958.1"
                     /translation="MFLKDLFVDFPFSRPSNIPDVEINEIVIDSRAVKPGNLFIAMRG
                     RDADGHTYIQKAIDNGAAAIIGDKELSGFSVPYIQLENARHSLTWLAAAFHGFPARKL
                     TVIGVTGTDGKTTTSNLLYKILIAAGLKAGMISTVNAVIGDEVLDTGFHVTTPDAHDV
                     QRYLAKMVAAGLTHVVLETTSHGWSQHRIDACEFDIAVVTNITHEHMDEHGGYENYRA
                     AKARLFESLEWTRAKPQGNPRLGVINFDDKKSFDFLNDFIKTKKINYGLGEGSDVRGM
                     NIAYSPSGIHFTAVAKDIRVAVSSNLVGAYNVSNCLAALTAAVYGLGISPEIVAEGIA
                     SLEGIPGRMERIDMGQNFTAIVDFAHTPNALKVALEAGRTMTDKRVIAVFGSAGLRDK
                     EKRRMMAETSAELADLTVLTAEDPRIESLNGILEEMANGMKSKGGREGVNFWRVPDRG
                     EAIKFALTLAQPGDIVLSCGKGHEQSMCFGKTEYLWDDRTAMRAALADYLEVQGPAMP
                     YLPSQDTPEEEWLKG"
     gene            complement(6011..6730)
                     /gene="atpF"
                     /locus_tag="PLT08_00460"
     CDS             complement(6011..6730)
                     /gene="atpF"
                     /locus_tag="PLT08_00460"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000276 - mitochondrial
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_component: GO:0045264 - plasma membrane
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HNE02959.1"
                     /translation="MEALGINVGLLIVQLIAFTIVMLTLNAWVYKPMLDMMETRKKKI
                     SQGLEDARVAAEARANAEKDAAKIVADAQAEASKVVREATERAEVASKDVRTAAEGEA
                     AKAREAAMAEAELERGRILGDLRGQVAALAIAAANKLVGEALDEKKQRALLDEFFSGV
                     KSGKVVVVGEELKGDTAVVTSALPLSNDEQAAVKKSVVAKDYSFKVDPSILGGLVIKV
                     DEKVLDASVAGKLEGLRSALK"
     gene            complement(6748..6990)
                     /gene="atpE"
                     /locus_tag="PLT08_00465"
     CDS             complement(6748..6990)
                     /gene="atpE"
                     /locus_tag="PLT08_00465"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000276 - mitochondrial
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_component: GO:0045264 - plasma membrane
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0 subunit C"
                     /protein_id="HNE02960.1"
                     /translation="MDGQFLLDAMRFVGAGLAMIGAAGAGVGIGLSVQGALGGMARNP
                     DTYGNLLTNMILGVAFSESIAIYCLVIAFLMLFKVV"
     gene            complement(7046..8023)
                     /gene="atpB"
                     /locus_tag="PLT08_00470"
     CDS             complement(7046..8023)
                     /gene="atpB"
                     /locus_tag="PLT08_00470"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000276 - mitochondrial
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_component: GO:0045264 - plasma membrane
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HNE02961.1"
                     /translation="METQKRTPWRRWIVLVLMVAGAYLASVFVPVRPEITVAAEKLIE
                     EPLIENFLGQPFYLVNTLPTLAVTIVLLVVIAFATNRSLVKSQKTDLIPSGIGNAMEA
                     ILEMLYNLTESSAGAKWAKAIFPWFATIMIYVLFANLLKLIPGFESIGVIHHAHGEGH
                     AVQHMGGFDMILPEKGEYILAPFFRGISVDLNFTLTLAVISFVMIQVIGVRAQGLAYF
                     SKFFNTKRMFKVPFFGAMDFLVGLLEVISEFAKILSFTFRLFGNMFAGVVLVAVVASL
                     LGNVSILAAMIMMFELFVGLIQAFVFGMLTMVFMAMATQGHGDEEHAEH"
     gene            complement(8034..8315)
                     /locus_tag="PLT08_00475"
     CDS             complement(8034..8315)
                     /locus_tag="PLT08_00475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="AtpZ/AtpI family protein"
                     /protein_id="HNE02962.1"
                     /translation="MTQSDLEEKRKKMVYSLLFVVIGQVGFITLIVLFALVWGGVWLD
                     NYFGTKPVITFVLSIAGIPLSVFLMFMASRRALARFTEQYEAEKKKISA"
     gene            complement(8516..>10029)
                     /locus_tag="PLT08_00480"
     CDS             complement(8516..>10029)
                     /locus_tag="PLT08_00480"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit N"
                     /protein_id="HNE02963.1"
                     /translation="RDGSDGENWRGLHHDHLGRCIMFTSQTFASILPEILILTIGLLV
                     LIFEPFWKGEQKRNAGWLTAGGLLVAMLVSLIFARPGEPVAVLGGMLRFDWLGFFLKM
                     LFMFAAAATALLLMDHEKVGKRGEAYLMLLASLIGMNFMASAGDLIMLYLSIETASIP
                     LYILSGFLLDDEHSTEAGFKYFLYGAMSSTIMLYGFSLVFGFSGTTDLYAIATSLTSG
                     SISPLIAFGVVALVVVGLGFKVSIVPFHFWAPDVYQGAPTPVAGYLSTASKAAGFAVI
                     LRLFFVAFPEFSSTWAMILAVLAAISMTVGNLLALPQTNIKRLLAYSSISHAGYAMIG
                     AVAFSQFGAASVVFYLAAYIATNLLAFGIVMAFSRVTGLEEITDYAGLSRRSPLLGLM
                     MLAAFLSLTGMPPFGGFVAKVIVFAAGVQANYTWLVVVGIINSIIGVYYYLNVLKYVY
                     LYRMPEQDEENHPIPLTQPYMIALVVLAIGVILLGTVFAPFFNWSDAGALNLF"
BASE COUNT         2780 a         2596 c         2232 g         2421 t
ORIGIN      
        1 tctttggcag tgcgttgacc aacttcggcg tgcgcatgtt ccttgattcg ttcattcaat
       61 ttgcgcctcc acctcaacct tacctcagcg acacaggttt agtcctgcca gaagacactg
      121 agttcactgg ctttatcttt aagattcaag ccaacatgaa ccccaagcat cgtgacagcg
      181 tcgccttctt gcgcatctgt tctgggaaat tcgagcgcgg tatggatttg ctccatgcgc
      241 cctcaggaaa gactctcaag ctgatgcgcc cttacaaagc gtttgcgaat gaacgcgaga
      301 tcatcgaaga agcctaccct ggtgatgtgt tgggtctgcc taataatggg atcttctcca
      361 tcggcgatac tctctgcaca ggcaaaacga ttcagttcgc gccgattccg cgcttccaac
      421 ccgagcattt tgctctgctg cgaaatgtgg atgtaagcaa acaaaagcaa tttgccaaag
      481 gtctacagca gctcgaaagt gaaggcgcag tgcaaatcct gtataacgtc aacgctttca
      541 agcgtgagcc gatccttgcc gtggtcggtc aactgcaatt cgacgtggta caagcccgcc
      601 tcgaatcgga atacaacgtc aagacggagt tggaacgcct caaccacagt cacctgcgct
      661 gggttcttgg tgaagataaa gatattgaag ctctcgccaa ccgcggagat gccattctgg
      721 caagagactc tcgcaacgcc tgggcaatgc tctttcaaac cccgttcttg ctcaaatact
      781 attctgaaaa gaaccctaat ttgaaatttg tagaaattac cgagctttag cgtacacgct
      841 aaatatttta gggcgggcgg agacaacttc gcccgccttt catgttaaac ttctcactat
      901 ggacaagacc tcttttgaac gactcatcca cgaaagttgg gcaacttatt ttggctgttc
      961 agtcgaaaca acggaacagc ccggcactac gcttttgccg gaaagcaaat atgagggcga
     1021 taaagtcatt gccctttggc acatcggaaa acacagcttc gtgcaacttg acccagcata
     1081 ttattctcaa ctggaagatt taatcaaaaa gctgccacac catacttcat tgactggtga
     1141 acaaatcgaa gcagcatggg gtaaagatgc catatcttca caagatgttg gcttcatgca
     1201 ttatctcttt ccatcagact taccccatta ccaaccctca ttgcctttta cactgcgcca
     1261 actgaccgaa gcggacgcgg gtcttatgtc tactttacag gcggtgaact cacccgaaga
     1321 tgtcgatgaa gcctttgtgg aagtgactca cgaaattgca ttcggctgtt ttcacggcga
     1381 agaacttgtt gctgctgcca gcggctacga gcgaacaggc tttctcgacc tcggcgtgct
     1441 aacacaccct gattaccgta aaaaagggct aggcaaagct gtggttggtg caacttgcaa
     1501 ttgggcaaac caaaaaggga taatcgctca atatcgtcac aacgcgctta ataccagttc
     1561 acaaaatgta gctacgtctt tgaactttca aaaatacttt agttcagaaa gcttttttct
     1621 caggtaactt cgtggcatac ctcaaaactt tatataacca gtatccacgc cagttctggt
     1681 tgatgatcct tgggattgtc atcagtactg caggcggcgg aatggtctgg cccttcatgc
     1741 ttatctacgc cggaggcaaa ctcggtcttc cgctcagtgc ggtggcgtcg ctgatctcta
     1801 ttaatgcagg tgtaggcttg ttcgcatctt ttctcgccgg aactttagca gaccgcattg
     1861 ggcgtaaagt ggtgatggtc tccagcctcg ttatgatggg tgtcgcctat cttctcctca
     1921 tcaaagcaga gacttacctt caatttgcat tactaatggg catggttggg ttgtcaaacc
     1981 cgctttatca agtgggtgcc gatgccatgt tggcagacat gattcccact caaaagcgaa
     2041 ccgacgccta tgccatcaac cgtatcgcct acaatgcctc gtttgggatt ggtccagcga
     2101 ttggtgggtt tcttgcgtcc acttcgtatg cgctcgcatt ttacggggca tctacaggat
     2161 tctttatcta tgggattatc tggtttttct tcgcgcacga aacgcttgat aaaaccgtcc
     2221 gcgaagaaaa agcttccact ttagccgggc aggctcagat ccagaatgaa gcggcgcgat
     2281 ccgatgggta tatccacgtt ttcaaagaca aggcatacat ggcgttcgtc actttatttg
     2341 caatcggtat gatcgcgcct tctatcttat ggattcttct accggtatac accaaaacca
     2401 acttcaattt acccgaagcg ctatatggtt ggattccaac cacaaacgca ttcatgtgcg
     2461 tgttcttgca atatgccgtt tcgaaccaaa cgcgtaaatt caaaacccta ccggttgcaa
     2521 gcgttggcat gcttgtgtat gcgcttggtg ccggcagtgt ggcgctcatg acgggcttta
     2581 cagggttttg gctaagcatg gtcattctca cgtttggcga gctaattctg attccaatcg
     2641 ccagcaaata tgtggcagat atcgcaccag aaaatttacg cggaagatat atgtcacttt
     2701 attggtttgg ctggggcatg tcgcgcacac ttgcgccgct aatcggtggc tatctgaatg
     2761 acaacatctc accacgcgca atttggattg gaggtctgct cttcggcttg accagcaccc
     2821 tcggcttgat tctactcaac agcctgacca acaccccaac agcgcttcaa actcgaccca
     2881 aaattgatac tacccatgga gattaactat gaagcaaact cttgcactga tttcttacgt
     2941 tgtccgtgat tatgatgaag cgattgcgtt tttcaccaat gctatgcaat ttgatttggt
     3001 cgaagatacg ctgcaagaga ataaacgctg ggtggtcgtc cgcccgcgtg gcggagttgg
     3061 cgtatcgctg ctgcttgcca gagctgatac acctgaacaa caagccgctg ttgggaatca
     3121 aactggcggg cgcgtttttc tattcttgca caccgataat tttaaaagag actatgagca
     3181 tatgcgctta cacagcatag aattcatgga agagcctcgt catgaagcct atggaaccgt
     3241 cgccgtattc aaagatttgt atggaaacaa gtgggatttg ctacaaccaa aataaaaata
     3301 ttgctattcg ttttttttca acatggcttc caggtttttc tttaccccaa cccattccga
     3361 atccaagatc gaatagaata ctgaatgccg aatgcgccca tccggcaaaa tcatgtgatt
     3421 acgcaagact ccttctttca ccgcgccaat gcgttcaatg gcagcctgag aacggaggtt
     3481 gatcgagtct gttttgatct gtacacgaat ggttttaaga acttcaaaag catgggttag
     3541 caagaggtat ttgcattctg tgttcacagc cgtacgttgg aattcttttc cataccaggt
     3601 gccacctact tcaagtccgc gatctttggg tatgatattc aggtaacgag ttgcacctgc
     3661 caccttgcca gatgcaagat gaataacgac aaaagggaga tcaccacctt ttttctcgcg
     3721 ctccagaata tccaataccc aatttcgcat atcggcttcg gtcttcatat ctccatacaa
     3781 catgaagctc caaaaatcct gcccagcacc gatctctgcc aaccccgata tatgttgttc
     3841 gcccatcggt tcgagacgca catatttccc ttgaagcgtg acacgctgag cccaatttgt
     3901 catttcaggt tcccaaataa ctcggtagtc ttttgggtgc gaaagtcaaa gattgaatct
     3961 aaccgtcttc tcacccaaac cgcatggaca atatctccca acaactcaaa cggcaacatg
     4021 tacgtcacac ggtcggtcat cttaacgcca ccattcatac tctcaaagat gtgttcatga
     4081 tgccacaacc gatatggacc tattcgttgt tcatccacaa aaaactcacc ttcgcgaaca
     4141 tgcgttatct ccgtcagcca taacgaacga atgccacgga taaactccac ccgatattcg
     4201 atcatctgcc cggggtacat ttttctatct ccgcccttaa taatcttaaa gttcatatca
     4261 ggcggagtaa tcgtattcaa attgtatggg ttacaaaaaa attcccagat atctttcatg
     4321 gttccgggaa ttacttgctc tcgttctaaa taatgaatca tcccttcaac cattcttctt
     4381 ctggtgtatc ttgcgaagga agataaggca tggcaggtcc ctgcacctca agataatccg
     4441 caagagcggc acgcatggca gtgcgatcat cccataaata ctcggtcttg ccaaagcaca
     4501 tcgactgttc gtgccccttt ccacaggaaa ggacaatatc gcctggttgg gcaagtgtca
     4561 gcgcaaactt gatagcttcg ccacgatctg gcacacgcca gaagttcaca ccttcgcgtc
     4621 cgcccttgga cttcataccg tttgccattt cttcaaggat tccattcagc gactcaatgc
     4681 gtgggtcttc agcagttagc accgttaagt cagctaactc tgcagaagtc tctgccatca
     4741 tacggcgctt ttccttgtca cgaagtcctg ccgaaccaaa gacagcgatg acacgtttat
     4801 ctgtcatcgt tctgccggct tctaatgcaa ctttcaatgc attaggtgtg tgagcaaaat
     4861 ccacaatggc agtgaagttc tgccccatat caatgcgctc catacggcct ggaatccctt
     4921 ctagagaagc aataccttcg gcaactatct ctggagaaat gccaagccca tacactgccg
     4981 cagtcaacgc ggcaagacaa tttgagacgt tatatgcgcc aaccaaatta cttgataccg
     5041 ctacacgaat atctttcgct accgccgtaa agtggattcc agatgggcta tatgcgatat
     5101 tcatcccgcg aacatcgctc ccttcgccca gcccatagtt gattttcttt gtcttgataa
     5161 agtcgttcag aaaatcaaag gactttttat cgtcgaagtt gatcacaccc aaacgcggat
     5221 ttccttgcgg cttagcacgg gtccattcga ggctttcaaa taatctcgcc ttggcagcgc
     5281 gatagttttc atagccgcca tgttcgtcca tatgttcgtg tgtaatgttg gtcacaaccg
     5341 caatatcaaa ttcgcaggcg tcgatacgat gttgagacca accatgagac gtggtttcca
     5401 gcacaacatg ggtcaagcca gcagcaacca tttttgccaa ataacgttgc acatcgtggg
     5461 cgtctggtgt ggtgacgtgg aaaccagtat ccaacacctc gtcaccgatt acggcattca
     5521 ccgtagagat catgcctgct ttgagtccag ccgcgatgag gattttgtag agcagattac
     5581 tggttgtcgt tttgccatcc gtgccagtta cgccgatgac ggtcagttta cgggcaggaa
     5641 agccatgaaa cgccgcagca agccaggtca aggaatgacg cgcgttctcc aactgaatgt
     5701 acggaacgct aaatccgctc agttctttat ccccaataat cgcagcagcg ccattgtcta
     5761 ttgctttttg aatataggta tgcccatctg catcacgtcc gcgcatggcg ataaatagat
     5821 tacccggctt tacagcccgg ctatcaatga ctatttcatt tatttcaaca tcagggatgt
     5881 tagaaggacg agaaaaggga aagtcaacga acaagtcttt gagaaacata gttgctagtt
     5941 tatctccatt gaagttaaac aaaaaaggct ttggtaattt taccaaagcc tttttgatat
     6001 cgttttagtc ttacttgagt gcagagcgca gaccttcaag tttaccggcg acggaagcat
     6061 cgagcacttt ttcgtcaacc ttgatgacca aaccaccaag gatggaaggg tcaactttga
     6121 aggaataatc cttggcaaca acactcttct tcacggcagc ctgttcgtca ttggaaagag
     6181 gaagagcact ggtgaccacg gcggtatcgc ccttgagttc ttcaccaaca acaaccacct
     6241 tgccagattt cacgccagag aagaattcgt cgagcaaagc gcgctgtttc ttctcatcaa
     6301 gtgcctcgcc aacaagcttg ttggcagcag caatagcaag agcagcaacc tgaccacgaa
     6361 ggtcaccgag aatgcgaccg cgttcgagtt cagcttcagc catagcagct tcacgagcct
     6421 tggcagcttc gccttcagca gcggtgcgga cgtccttaga agcaacttcg gcgcgttcgg
     6481 tagcttcacg aacaaccttg ctagcttcag cctgggcatc agcgacgatt ttggcagcat
     6541 ctttttctgc gttagcgcga gcttcggctg caacacgggc atcttccaag ccctgagaga
     6601 tcttcttctt gcgggtctcc atcatatcca gcatgggctt gtagacccaa gcgttcaacg
     6661 tgagcatcac aatagtgaac gcaatcagct gaacaatgag aagaccgaca ttaataccta
     6721 atgcttccat gttttatgct ccttgaatta cacgactttg aagagcatga ggaaggcgat
     6781 gaccaagcag taaatagcaa tggattcaga gaacgccacg ccaaggatca tgttggtcaa
     6841 caagttaccg taggtatcgg ggttacgagc cataccacca agagcaccct gcacgctcaa
     6901 accgataccg acaccagcgc cagcggcgcc gatcatcgcc aagcctgcgc ccacgaagcg
     6961 catcgcatct aaaagaaatt gtccatccat gtttcgagtc tccttaggtt tatcctagtt
     7021 aattatgaat tatgtttcaa aaaaattaat gttctgcgtg ctcttcgtcg ccatggcctt
     7081 gtgtcgccat tgccatgaac accatggtca acataccgaa cacgaacgcc tggatcaagc
     7141 ctacgaacaa ttcgaacatc atgatcattg ctgccaaaat gctgacatta cccaacaaag
     7201 aggcaactac cgccaccaac accacacctg cgaacatgtt accaaacaga cggaaggtaa
     7261 acgagaggat cttcgcaaat tcagagatga cttcgagcaa gcccaccaaa aagtccattg
     7321 caccgaagaa aggcaccttg aacatgcgct tggtattgaa gaacttgctg aaatatgcca
     7381 aaccttgcgc gcggacacca atcacctgga tcatcacaaa tgagatcaca gccagtgtga
     7441 gggtgaagtt caagtccaca gaaataccgc ggaagaaagg agcaagtatg tattcgcctt
     7501 tttcaggcaa aatcatatcg aacccaccca tatgctgaac agcatgacct tcgccatgag
     7561 catggtgaat cacaccaatc gactcaaaac cagggatcag cttgagcaag tttgcgaaca
     7621 acacatagat cataatagtc gcaaaccaag ggaagatcgc cttcgcccat ttggcgcctg
     7681 ctgagctttc agtcaggtta tacagcatct caaggatggc ttccattgca ttaccaatcc
     7741 cagaaggaat caaatctgtc ttctggctct tcacaagaga gcgattggtc gcaaatgcaa
     7801 tcaccactaa caggacaatg gtcacagcca aagtgggaag cgtgttcaca agatagaacg
     7861 gttgcccaag gaaattctca atcagaggtt cctcgatcaa tttttcggca gccactgtaa
     7921 tttcaggtcg aaccggtaca aaaacgcttg cgagataagc gcccgcaacc atcaaaacca
     7981 gaacaatcca gcgccgccag ggggtacgtt tttgggtttc caagccccgc ctcctaagca
     8041 gaaatttttt tcttttcggc ttcatactgt tctgtaaacc gcgccaacgc tctgcgcgaa
     8101 gccatgaaca tcagaaaaac cgaaagtgga ataccggcta tcgacaatac gaacgtaatc
     8161 accggtttcg ttccaaaata gttatcaagc cagacaccgc cccaaaccaa ggcaaatagc
     8221 acgattaggg taataaaacc aacctgccca atcactacaa aaagcaggct atataccatt
     8281 ttcttgcgtt tttcctcaag gtccgattgt gtcatagcgc acgaattata actgacaggt
     8341 ctaagagcgt caaacggaaa tgaaaaatcc cttttggagg gattccccac ccgcagagcg
     8401 attccgcgcc cgaacaacgc ctcttaacta gccgattctt gcctctattt tatgaggctt
     8461 ttcctacttt gatacaaagt gacagtcacc ttgcggtgac tgtcacttga tttcactaga
     8521 acaaattcaa cgcaccggca tcagaccagt tgaagaatgg agcaaagacc gtaccgagta
     8581 gaatcacgcc gattgcaaga acaaccaatg cgatcatgta tggctgagtc agcggaatgg
     8641 gatggttttc ttcatcctgt tcgggcatgc gatacaggta cacatacttc agcacattca
     8701 agtagtagta cacaccaatg atcgagttga taataccgac aacaaccaac caagtgtaat
     8761 tggcttgaac gcctgcagca aagacaatca cctttgccac gaaaccaccg aagggcggca
     8821 taccggtcag cgagaggaag gcggcgagca tcatcaagcc gagcaagggg ctacggcgac
     8881 taagacccgc atagtcagtg atttcttcca aacctgtgac acggctgaac gccattacga
     8941 taccgaaggc gagcaagttg gtagcaatgt aagctgcaag gtaaaagacc acacttgccg
     9001 caccaaattg actaaacgcc actgccccga tcatggcata gcccgcatgg gaaatggaag
     9061 aataagccaa gagacgcttg atgttagtct gaggcagcgc cagtaaatta cctaccgtca
     9121 tggaaatggc agccaacaca gcaagaatca tcgcccaagt actgctaaat tcagggaagg
     9181 caacaaagaa caggcgcagg attactgcaa aaccagccgc ttttgaagcc gtggaaagat
     9241 acccagctac tggtgtagga gcaccctgat atacatccgg tgcccagaag tggaatggca
     9301 caatggaaac cttgaagccc aaaccgacaa cgaccaaagc aactacgcca aaggcaatca
     9361 acggggagat tgaacctgag gtcaacgagg tagcgatggc atacaagtct gtggtgccag
     9421 agaaaccaaa gaccaagctg aagccataca acataatggt ggaagacatc gcaccataca
     9481 agaagtattt gaaacccgct tccgtagagt gttcatcatc cagcaagaag ccggaaagaa
     9541 tgtaaagcgg aatggacgcc gtctcaatgg agaggtagag catgatcaag tcaccggcag
     9601 aagccatgaa gttcataccg atgagagatg caagcaacat gagataagct tcaccacgct
     9661 tgcccacttt ttcatggtcc atcaaaagaa gggctgtggc ggcagcggcg aacatgaaga
     9721 gcattttgag gaagaagccc agccaatcaa agcggagcat gccacccaaa actgctacgg
     9781 gctcaccggg gcgagcaaag atcaagctga caagcatagc gaccaataaa ccaccagcag
     9841 tcaaccagcc ggcattgcgc ttctgctcgc ctttccagaa aggctcaaag ataagaacca
     9901 aaagcccaat ggttagaatc agaatttcgg gaagaatgga ggcgaaagtc tgtgaagtaa
     9961 acattatgca cctcccaaga tggtcatgat gtaatccacg ccagttttca ccatcggaac
    10021 catcacgga
//
LOCUS       DAOQJE010000010         9950 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_3565_length_9950_cov_6.374128, whole genome shotgun sequence.
ACCESSION   DAOQJE010000010 DAOQJE010000000
VERSION     DAOQJE010000010.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 9950)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9950)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9950
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3565_length_9950_cov_6.374128"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..413)
                     /locus_tag="PLT08_00485"
     CDS             complement(<1..413)
                     /locus_tag="PLT08_00485"
                     /inference="COORDINATES: protein motif:HMM:NF024583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HNE02964.1"
                     /translation="MASNLKTQNERLSALYSIALDLLHRQSLEDVLKTILSSASELLD
                     SPIGFLDLIDGEMLIIQAATPMVSNYLGMRVPIHKAAVTERAIQTRSPQFIKNYMKRR
                     SRIKEYDPFQMMSACTFPIMVGETVVGALSLGRVYP"
     gene            complement(564..8519)
                     /locus_tag="PLT08_00490"
     CDS             complement(564..8519)
                     /locus_tag="PLT08_00490"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HNE02965.1"
                     /translation="MPKNKTKMDIQAELEAAQKRVAELEEAFGSDRVEQRFASVFRAS
                     PSQMALTDQTTGRYIEVNEAFLNTLGFSREEVLGKTATELNLFVDPQQRQDLLLRMRD
                     QGFLRNEHVLVRAKTGEIRHGIFSAEFINTTEQKLLLTVMNDVTEKLQAEQRWQFALD
                     GADEGVWDWDPQTNKVFYSKRWKSMLGYEDGEIGNTLEEWSSRVHRDDLPRVMQEVTR
                     HLSGETPIYKSEHRILRKDGTYLWVLDSGKVMEWSEDKKPLRVIGTHKDISERKKAEE
                     SLRENEERFSRMFHANPAVQLIVKFDDGIVLDVNESFCLQTGFDRDELIGRSMRDLNL
                     WQDPFKQKRIMDELRSKGGVHDVELEFWTNTGDLRTLLISFDLVEIRGVLCNLATGVD
                     ITSRKQAERDLRFREKQYHSLVETLDVSICRWLPDTTLTYANEKYRKIFNITGNAEGK
                     KWIDLLPEAARASTAIFYSEVTKHPRTVMYDHPVTMNDGKIHHYHWVDTPILGNDGEV
                     LEFQSIGIDITDRKLSENALVASEKRLKSLVDSQSHYVIRVNMEGRYSYWNAQFEKEF
                     GWLYESEGIQNAFVLKAVCSYHHERVTNIVNQCIAEPGKVFTVEIDKPARNGSIRTTL
                     WEFICLTDELDQPYEIQCMGVEITGRKQAEDDLRKNEQMLRLFIEHSPAAIAMFDLEM
                     RYIIASKRYLIDYRLGDVDLVGRSHYEIFPEMTEEHRHIHQRCLMGEIDKKDEDLLVR
                     KDGSSDWVRYELRPWHQSNGEVGGLIFFSEVITERKLADEKLRESERKYRELINGMND
                     TVWVIDFDTRFLDVNDAAVKTLGYTREELLSMKVSDIDSGIEPEKIKQLIDRLKVEKR
                     QVFETSHITKNGRKIPIEVSSSLVSYMGEMVVMSIARDITDRKQAEEEARLAEQRYRS
                     LIENAPDGIVLVGIDGHFKYASPSVRRLFGYSIDETLQSMPDQFTHPEDLPGVLKELE
                     HLVADPTYIPTLQYRFLHKNGEWRWIESTFSNLVSVPSVEAIIINFRDIHERKLAEEA
                     LTKSQALLTEAQRIGRIGHMEWNGSGQVLICSDELYDILEIPYEAQISKEIISNMMKP
                     GEAERIHELDMQAIQNHTDMDYEYCIQLKDGRERWLHQQGKFSYNQDGYPVRMLSIVQ
                     DITERKQTEEALRAEQARLAGIADTIPGAILTFQLCPDGSFNIPYASKAFDDLYGLSR
                     EDVRENIDFISARVPHDQLEMLLQSVNESAKTLQPWRNEYVYHHPTKGMIWIEGFSMP
                     MREADGTILWNGFASDVTERKTAEATLQESRLRLEMALKGANAGMWDWNVQTGETVFN
                     ERWAEIVGYSLQELEPISIQTWADLCHPDDLKLSDALLQKHFARETEFYECEVRMRHK
                     SGSWVWVVDRGKVMEWDANGKPLRMFGTHLNITERKLSEEEIRKRGEDLLLINTLNDA
                     ANRGEPLSNILNTLIQDARNMFDCQDLSVYLLSPDGKYAELQNLTLSGKRVERIEKLI
                     GRAIPRVQVPIREDGYFKQLLSNEQGTLIEDAASIRQWIEEFVDTPTLPDIVRPLIRK
                     AIPQIQKVIDINTIFAMPLFFSGNVIGLIELSSHTSFDKDDLKRIRTISRQMSAIILR
                     IWAVEKLRESEEKYRTLYKEMPIGVLLVSVSGEVLEANSATLQILGSPSIEATRQINL
                     LTFAPLIEAGLAADFKKTVDSEQINRVERHYLTKWGKSVHLYVTFVPVITPQQELVVQ
                     VLIEDITARKDAENNLRASEEKYRSLIKSLNNSISIVDANGTFLYLNDMAGKELGGPP
                     AELIGKTMHDLFPEPFSSMQLELVRNVIKIDHGYVYEAQSVVNNELRWFRVNFQPLHN
                     ENGEVNQVLINANDIHDLKVAQDELQKLNRNLEERVKERTAEVQDLYENAPTGYHSID
                     ADGKFVRVNKTILDWLGFARDEMMGSPISGFMTSATQKIFSEKFSELVQHGLIKDVEI
                     ELIRKDGSILPVLLNATAIRDASGNFVMSRSTVFDNTERKQAEIELKRNVNFTSALLN
                     AVPTPVFYKDNEGRYLGCNQSFVNMMGKTAEEIYGKLPHEIWESSHADLYRQKDIELF
                     QSNELQTYEGSIRDKHGNLHSAIFVKNVFLDESGKTAGLVGAFIDITERKQAELAIRE
                     SEATYRALFENSNDGIFLMAPTGEELRANQHALDLVGYTLDEYLNMGGVSQNPFTLDD
                     DERKDADDKLAALIRGETVPLYERLFTAKDGHKVPVEINLSPVRDANGNLIMVQSVVR
                     DISQRKAAEDALRTINKELERALRVKDEFLANMSHELRTPLNGILGFSEILLSGEFGS
                     LSDRQHKYISSIEGSGKHLLGLINDLLDLSKIEAGKLDVMPENVIISDLCQSSLVFVK
                     QMALKKKIHLALEQDMTYPFIIADQRRLKQILVNLLSNAVKFTPENGKVTLRVAGDPA
                     RGCINFSVEDTGIGISPEDLKRLFTPFTQVDSSLTRQHEGTGLGLALVQKLAELHGGG
                     VAVQSEVGKGSTFTVFIPGNYETSLEEMHDHATTKAENKVAAMPTHLNSGKILLAEDI
                     ESNIVILGDYLEFQGYTMVYARNGKEALDKAKETLPDLILMDIQMPVMDGLEATRRLR
                     TDSRFATVPIIALTALAMSGDRERCIEAGATEYVSKPMSLKDLTDLIQELIKKNK"
     gene            complement(8506..8721)
                     /locus_tag="PLT08_00495"
     CDS             complement(8506..8721)
                     /locus_tag="PLT08_00495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE02966.1"
                     /translation="MFMPSCVSDNVTNYVGIVLKRRLCSIFYKVDEEYHDNYGIIVVV
                     IKGKLTNNSLLDLEKMPIVPGDIYAQK"
     gene            complement(8793..9656)
                     /locus_tag="PLT08_00500"
     CDS             complement(8793..9656)
                     /locus_tag="PLT08_00500"
                     /inference="COORDINATES: protein motif:HMM:NF017206.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HNE02967.1"
                     /translation="MKVLVTGATGFTGSRLVPLLLQNGYQVRVFVRPTSDRSPLSALT
                     VEWATGDFANPETFTAAMHGVDALVNIASLGFGHAESILKSMKEAGVRRGIFISTTAI
                     FTQLNAVSKSIRLAAEEAIQTSGLDYTILRPTMIYGSKRDRNMWRLIRLLRITPIMPI
                     FGDGESLQQPIFVDDVAQAVLLALKNDMTIGKSYNIAGKAPLSYNQVIDTVASALGKR
                     VWKLHMPYMTIVRALQFTERMKLRLPIKAEQVLRLNENKAFSYEEAQKDFDFNSRSFE
                     EGIRMEIQANK"
     gene            complement(9653..>9950)
                     /locus_tag="PLT08_00505"
     CDS             complement(9653..>9950)
                     /locus_tag="PLT08_00505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE02968.1"
                     /translation="PAFRTTYPNKVFDYMAAGRASIIVIDGITRDLIEASRAGVYVNP
                     ADDAMLAQKILDLSRNPAQVKAMGANAREYLVKNLDRRDKLDETLGLLEKLVTK"
BASE COUNT         2581 a         2460 c         2031 g         2878 t
ORIGIN      
        1 gggtacaccc gccccaaact cagcgcgccc accacggtct cacccaccat gatcgggaag
       61 gtgcaagcag acatcatctg aaacgggtca tattctttga tgcgcgagcg gcgcttcata
      121 taattcttta tgaactgagg gctacgggtc tgaatggcac gctcggtcac cgccgcttta
      181 tggatcggca ctcgcatgcc aagataatta gaaaccatgg gcgttgccgc ttgaatgatc
      241 aacatctccc catcgatcaa atccaaaaaa ccaatcggcg aatctaacaa ctccgacgca
      301 ctcgaaagga tggtcttcag cacatcttcc aaactctggc gatgaagcag atccaacgca
      361 atcgaatata aagcagataa gcgctcgttc tgagtcttta agttggatgc cataaacgta
      421 cagcggattc tagcggaaac tcgcctcttt cttaagggat aacggactgc cccttaagtt
      481 ggatgtcttt gcatctcggg caggtttctg ctctcgtttt aaaagcaaaa gccgaaaggg
      541 aggtcccttt cggctttttt ggttcactta tttttcttga tcaattcctg aatcaaatcc
      601 gtgaggtctt tcaaactcat cggcttgctc acatactcgg tcgcgcctgc ttcaatgcag
      661 cgctcccggt cgccgctcat ggcgagggcg gtcagagcaa taatgggcac ggtagcgaag
      721 cgcgaatccg ttcgcaggcg gcgggtcgct tcgagcccat ccatcactgg catttgaata
      781 tccatcaaaa tcaggtctgg cagagtctct ttggctttat ccagtgcttc cttcccgttt
      841 ctggcataaa ccatggtgta gccctgaaat tcaagatagt cacccaaaat cacaatattc
      901 gattcgatat cttctgccaa aagaatcttg ccactattaa ggtgggtagg catggcggct
      961 accttgtttt cagcttttgt agttgcatgg tcatgcattt cttccaaact ggtttcatag
     1021 tttccgggaa taaaaaccgt aaaggtacta cctttcccaa cttcgctttg tactgcaaca
     1081 ccgcctccat gtaactctgc taatttctgt accaatgcta accccaaccc ggtcccttcg
     1141 tgttgacggg taaggctgct atccacttgg gtaaatggag tgaataagcg cttcaggtct
     1201 tctggcgaaa tgccaatgcc ggtatcttcc accgagaagt tgatgcagcc tctggctgga
     1261 tcgcctgcca cgcgcaaggt cactttgcca ttttcagggg tgaacttcac ggcattgctc
     1321 aacaggttca caagaatctg tttcaggcgg cgttggtcgg caatgatgaa aggataggtc
     1381 atatcctgtt cgagcgcaag gtgaattttc ttcttaagcg ccatctgctt cacgaacacc
     1441 aaactcgact ggcacaagtc tgaaataatc acattttcag gcatcacgtc taatttcccg
     1501 gcttcgatct tggaaagatc aagcaggtca ttgataagac ccaacaagtg tttaccactg
     1561 ccttcaatag aagaaatgta tttatgttgg cgatcgctca aactgccgaa ctctccgcta
     1621 aggaggattt cggaaaagcc taaaataccg tttaaggggg tgcgaagctc gtggctcata
     1681 ttcgccaaga actcgtcttt tacccgcaac gcccgttcca attctttgtt gatggttcgc
     1741 aaagcatctt cagcggcttt gcgttgcgaa atatcacgca ccacgctttg caccataatg
     1801 aggttgccat tggcatctcg caccggcgaa aggttgatct ctacaggcac tttatgccca
     1861 tcttttgcag taaacaaccg ctcatacaat ggaaccgttt cgccgcgtat taatgcagcg
     1921 agtttgtcat cggcgtcttt tcgttcatca tcgtccaagg taaatgggtt ctggctgacc
     1981 cctcccatgt tcagatattc gtccaaggtg tagcctacca aatctaatgc atgctggttg
     2041 gctcgtagct cttctcccgt tggagccatc aagaaaatac cgtcattcga attctcaaac
     2101 aaggcacgat aagttgcttc gctttcgcga atggctaact ctgcttgctt tcgttcggta
     2161 atgtcaataa acgcgcctac caagccggct gtttttccgc tttcatccaa aaagacattc
     2221 ttaacaaaaa tcgccgaatg caagtttccg tgcttatcgc ggatgctccc ttcataggtc
     2281 tgaagctcat tgctttggaa taattcaata tctttctggc ggtataaatc tgcatgggag
     2341 ctttcccaaa tctcatgagg gagcttgcca taaatctctt cagcggtttt ccccatcata
     2401 ttcacaaacg attggttgca acccaaatat ctgccttcgt tatctttata gaaaacaggg
     2461 gtaggcacag catttagcaa cgcgctggta aagttgacat ttcgtttgag ttcgatctct
     2521 gcctgtttac gctctgtatt atcaaatact gtagaacggc tcatcacaaa attaccagag
     2581 gcatctcgaa tagcagtagc gttcaacaga accggaagga tggagccgtc ttttcggatc
     2641 aattcaattt ctacatcttt tatcaagcca tgttgcacca gctcagaaaa tttctcgcta
     2701 aatatctttt gcgtggcact ggtcataaaa ccagaaatag gcgaacccat catttcatct
     2761 ctggcaaatc ccaaccagtc taaaattgtt ttattaaccc gcacaaactt tccatctgca
     2821 tcaatagaat ggtagcccgt tggcgcattt tcataaaggt cctgcacctc tgcggttctt
     2881 tcttttacac gttcttccaa attacggttc agtttttgta gttcgtcctg cgctacttta
     2941 aggtcatgaa tgtcgttggc attaatcaag acttgattta cctctccatt ttcattatgc
     3001 agaggctgaa aattaacccg gaaccatcgc aattcattgt taactacact ttgcgcttcg
     3061 tagacatatc cgtgatcaat cttgataaca ttgcgcacta attccaattg catggaagaa
     3121 aatggttctg ggaataaatc gtgcattgtt ttcccaatta attccgctgg aggtccaccc
     3181 aactccttac ctgccatatc attcaaatac aaaaaggttc cgtttgcatc tacgatagaa
     3241 atagaattat ttaagctctt gatcaaactg cgatattttt cttcgctggc gcgtagatta
     3301 ttctctgcat ctttcctagc tgtaatatct tcaatcaaga cttgaacgac cagttcctgt
     3361 tgtggagtga tgaccggcac aaaggtcaca tacagatgaa ccgactttcc ccatttggtt
     3421 aagtaatgtc tttctactct attaatctgt tcagaatcta cagttttctt gaaatccgca
     3481 gccagcccag cctcgatcag aggcgcaaaa gtcaacaggt tgatttgccg cgtagcctct
     3541 atcgatggag aacctaaaat ctgcaaggtc gccgagttgg cttccaagac ctctccgctc
     3601 acacttacca acaaaacacc aatcggcatc tctttataca aggtgcgata tttttcttcg
     3661 ctttcacgga gtttttccac tgcccagatc cgcaaaatga tcgccgacat ttggcggctg
     3721 atggtacgaa tccgcttcag gtcgtcttta tcaaaggatg tgtggctcga aagttcaatc
     3781 aagccgatga cattccctga gaagaagagc ggcatcgcga agatcgtatt aatatcgatc
     3841 actttttgaa tttgcggaat cgcttttctg attaatggac gcacgatgtc tggcaaggta
     3901 ggtgtatcta caaattcttc aatccactgt cggatcgaag ccgcatcttc gatcagtgtg
     3961 ccttgttcgt tggataaaag ctgcttgaag taaccatctt cgcgaatggg gacttgcaca
     4021 cggggtatcg cgcgcccaat caatttttcg atccgctcaa cgcgtttacc agaaagggtt
     4081 aagttctgaa gctcagcata cttcccatct gggcttagta aatataccga caagtcctgg
     4141 caatcaaaca tattgcgtgc atcttgaata agcgtattca agatatttga taaaggttca
     4201 ccccggttgg cagcatcgtt caaggtgttg ataagaagca agtcttcgcc gcgcttccgt
     4261 atctcttctt cagacaattt cctttcggta atattcaggt gcgtaccgaa catccgcaaa
     4321 ggctttccat tggcatccca ttccatcact ttgccgcggt ctaccaccca aacccatgag
     4381 ccacttttat gacgcatccg aacttcacat tcgtaaaact ccgtctctct ggcaaaatgt
     4441 ttttgcagta atgcatcaga gagtttcaga tcatctggat ggcataaatc tgcccaggtc
     4501 tgaatactga tcggttcaag ttcttgcaaa gaatagccta cgatttctgc ccaacgctca
     4561 ttgaaaacag tctctcctgt ttgcacattc caatcccaca tgccagcatt ggcacctttc
     4621 aatgccattt cgagccgtaa gcggctctct tgcagggtgg cttcagccgt ctttctttct
     4681 gtaacatcag aagcaaaacc gttccacaaa atggttccat ccgcttctcg catgggcatg
     4741 gaaaaccctt caatccaaat catccctttt gtaggatggt gatacacgta ttcatttcgc
     4801 caaggttgca atgttttcgc tgattcattt acactctgca acagcatttc cagttggtca
     4861 tgaggaactc ttgctgaaat aaagtcaata ttttcgcgaa catcttcacg agaaagccca
     4921 tatagatcat caaaggcttt actcgcatac ggaatattga aggacccatc tgggcaaagt
     4981 tgaaaggtaa gaatagctcc aggaatcgta tctgcaatcc ctgccaagcg cgcttgttcg
     5041 gctcgtaaag cttcttctgt ttgttttctc tccgtaatat cttgcacgat cgataacatg
     5101 cgaacaggat atccatcttg attataagaa aactttcctt gttggtgaag ccagcgctca
     5161 cgtccatctt ttaattggat acagtattca taatccatat ccgtatgatt ctgaatggct
     5221 tgcatatcca gctcgtgaat gcgttctgct tcccccggtt tcatcatgtt tgaaatgatt
     5281 tctttagata tttgtgcctc gtacggtatc tcaaggatat cgtaaagctc atctgaacat
     5341 ataagaactt gtccgctgcc gttccattcc atatgaccaa tacgcccaat gcgttgagcc
     5401 tcggtcagca atgcctgact tttggttaaa gcttcttcag ctaatttccg ctcatgaata
     5461 tcacggaagt taataataat ggcttcaacg ctgggcacag aaaccaaatt actgaatgta
     5521 ctttcaatcc aacgccattc tccattttta tgaaggaaac ggtattgtag ggtcggtata
     5581 tatgttgggt ctgcgaccaa atgctcaagt tctttcaaca ccccgggcag gtcttcagga
     5641 tgagtaaatt gatccggcat gctctgtaac gtctcatcga tactgtaacc aaagagcctt
     5701 ctcaccgatg ggctggcata cttaaagtga ccgtcaatgc ctactagcac aattccgtct
     5761 ggggcatttt caatcaggga gcgataacgt tgttcagcga ggcgagcttc ttcttccgct
     5821 tgtttacggt cggtaatgtc acgtgcaata ctcattacca ccatttcccc catataggaa
     5881 accaaactac tactaacctc gatgggtatc tttctgccat tctttgtgat atggctagtt
     5941 tcaaatacct gtcttttttc tacctttaat ctatcaataa gttgcttgat tttctccggt
     6001 tcaattccag aatcgatatc tgagactttc atggagagta attcttctcg tgtatagccc
     6061 aatgtcttta cagcagcatc attgacatcc agaaaacggg tatcaaaatc aatcacccaa
     6121 acggtgtcat tcatgccgtt gataagttcg cggtattttc tctcgctttc gcgtaatttt
     6181 tcatctgcta gttttcgttc ggtaattact tcagaaaaga aaatgagccc gcccacttcg
     6241 ccattcgatt gatgccaagg gcgaagttca taacgcaccc aatcagagga accatcttta
     6301 cggacaagta aatcttcatc tttcttatcg atctcgccca tcagacagcg ctgatggata
     6361 tgccgatgct cttcggtcat ctccgggaaa atctcatagt gagaacgtcc caccaaatct
     6421 acatcgccaa ggcgataatc aatgagatat cgtttactgg caatgatata acgcatctca
     6481 aggtcaaaca tggcaatggc agctgggcta tgctcaatga agaggcgaag catttgctca
     6541 tttttacgta aatcatcttc tgcttgttta cgccccgtaa tttcaacgcc catgcattga
     6601 atttcataag gttggtctaa ttcatcggta agacaaatga actcccacaa tgtagtgcgg
     6661 atactaccgt ttcgggcagg tttatcaatt tcaacagtga atacttttcc gggctcagca
     6721 atacattgat tgacaatatt tgtcactcgc tcatggtgat aactacaaac tgctttaagt
     6781 acaaaggcat tctgtatccc ttcagattca tatagccacc caaattcttt ctcaaactgc
     6841 gcattccaat aggaatatct tccctccata tttacgcgga tcacataatg agattgagaa
     6901 tccaccaaag atttcaatct cttttcactg gcaactaatg cattttctga gagctttcga
     6961 tccgtaatat cgatgccaat cgattgaaat tcaagtactt caccatcatt ccctaaaatt
     7021 ggagtatcca cccaatgata atgatggatt ttcccatcgt tcatcgtaac cgggtgatca
     7081 tacatcactg tgcgagggtg tttagtcacc tcactataaa aaatggcagt agaagcacgt
     7141 gccgcctctg gtaaaagatc aatccatttt ttaccttcgg catttcccgt aatattaaat
     7201 atttttctgt acttttcatt ggcatatgtc aatgttgtat ccggcagcca acgacagata
     7261 ctgacatcca gcgtttcaac aagtgaatga tattgtttct ctctaaaacg caaatcccgc
     7321 tcggcttgtt tgcgtgaagt aatatccaca cctgtggcga ggttgcacaa tacccctcta
     7381 atttcaacaa ggtcaaatga aatgagtaaa gtacgaaggt caccggtgtt ggtccagaat
     7441 tcaagttcaa catcatgcac tccaccttta gaacgcagtt cgtccatgat gcgcttttgt
     7501 ttaaacggat cctgccataa atttaagtca cgcatagaac gaccgatcaa ctcatcacgg
     7561 tcaaaaccag tttgcaggca gaacgattcg tttacatcta aaacaatgcc atcatcaaac
     7621 ttaacaatca gttgtacagc ggggttggca tggaacatac gtgaaaaacg ctcttcattt
     7681 tctcgtaatg attcctctgc tttttttcgc tcagatatat ctttatgggt tccgatcaca
     7741 cgaagcggtt ttttatcttc agaccactcc attactttgc cactatccaa tacccacaaa
     7801 taagttccgt ccttacgaag gatacgatgc tcactcttat aaatcggtgt ctctccagaa
     7861 aggtgccttg tcacctcttg cattactctg ggcaaatcat cgcggtgaac gcggctagac
     7921 cattcctcta acgtattgcc aatttctccg tcttcatatc ccagcataga cttccaacgt
     7981 tttgaataaa agactttgtt ggtctgtgga tcccaatccc ataccccttc atcagcacca
     8041 tctaatgcaa attgccagcg ttgctccgcc tgcaattttt ctgtcacatc gttcataaca
     8101 gtaagaagca atttttgttc agttgtattt ataaattctg ctgaaaaaat accatgtctg
     8161 atctcccccg tttttgcacg cacaagtaca tgttcattcc ttagaaatcc ttggtctcgc
     8221 atccgcaaga gcagatcctg cctttgttgt gggtctacaa acaagttcaa ctcagttgca
     8281 gtcttaccaa gtacctcttc tcgcgaaaat cccaaggtat tcaaaaaagc ttcgttaact
     8341 tcgatatacc ttccagtggt ctgatccgtc agtgccattt gagatgggct tgctcgaaac
     8401 acactggcaa aacgctgttc aacccgatca ctcccgaacg cttcttcaag ctcagccact
     8461 cgcttttgag ccgcttccaa ttcagcttga atatccatct tggttttatt tttgggcata
     8521 aatatctcca ggcacgatgg gcattttttc taaatctaga aggctattat ttgtcagttt
     8581 cccttttatc actactacaa taattccata attatcatga tattcttcat caactttata
     8641 gaatattgag caaagccttc tttttagcac aataccaaca taatttgtta cattatcact
     8701 tacgcaagac ggcatgaaca tggggggatt ccccacctac gttttagcta attccccctc
     8761 ttctattcgc ttatagaata gaataatcca ctttatttat ttgcctgtat ttccattcga
     8821 atcccctctt caaaactacg cgagttgaaa tcaaaatctt tttgagcttc ctcataggag
     8881 aacgctttat tctcgttcaa acgaagcact tgttcagctt tgatcggcaa gcgcagtttc
     8941 atccgctcgg tgaattgcag agcacgaacg atggtcatgt agggcatgtg caacttccac
     9001 acacgtttgc caagcgcaga agcaaccgtg tcaatgactt ggttatagct caacggagct
     9061 tttccggcga tgttatagct tttaccaatg gtcatatcgt tcttcaaagc gagcagtacc
     9121 gcttgagcaa catcatctac aaaaatgggc tgctgcaaag attcaccgtc accgaagatg
     9181 ggcataatgg gcgtgatgcg caacaaacga atcagccgcc acatgttgcg gtcacgttta
     9241 ctgccataga tcatggtagg gcgcaggatg gtgtagtcca atccgctggt ttggatagct
     9301 tcttcggcgg caaggcggat ggatttgctg accgcgttca gctgggtgaa gatggcggtg
     9361 gtgctgatga aaattccgcg cctcacgccc gcctctttca tggactttag aattgattct
     9421 gcgtgcccga agccgagcga tgcgatgttg acaagagcat ctactccatg catggcggca
     9481 gtgaatgttt cagggttggc aaagtcacct gtcgcccact ccactgtcag cgcggaaaga
     9541 ggcgaccggt cgcttgtggg gcgcacaaag acgcggactt gatatccgtt ttggaggagg
     9601 agagggacaa ggcgtgagcc ggtgaatcca gttgcgcctg taacaaggac tttcatttcg
     9661 tgaccaactt ctctaacaag ccaagtgttt catctaattt atcgcggcgg tctaaatttt
     9721 tgacgagata ctcgcgggcg tttgcgccca tcgctttgac ctgagcggga ttgcgagaga
     9781 gatcgagaat tttttgcgcg agcatggcgt catcggcagg gttgacatag acgccggcac
     9841 gcgaggcttc gatgaggtcg cgggtgatgc cgtcgataac aatgatacta gcgcgaccgg
     9901 ctgccatgta atcgaaaact ttgttagggt aagtggtgcg gaaggcgggg
//
LOCUS       DAOQJE010000011         9913 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_3592_length_9913_cov_7.713431, whole genome shotgun sequence.
ACCESSION   DAOQJE010000011 DAOQJE010000000
VERSION     DAOQJE010000011.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 9913)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9913)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9913
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3592_length_9913_cov_7.713431"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            134..2815
                     /locus_tag="PLT08_00510"
     CDS             134..2815
                     /locus_tag="PLT08_00510"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HNE02969.1"
                     /translation="MLSENSELAHQGSTEVQRARKTARSTFFTALKFMATRLLTVLLT
                     IGLGTFITIVAANGGGAIDNAEKSRINAVTLRSVDPFATSAEELTKLRADMEEKEGLN
                     LPFLPRHMYWTYKALTLNWGEVNDRVKFHPRYTTSNMNAEDSRVILMQYLPNTILLIG
                     AGFFFLFIAGIPFALHLSQNPGGRLDKLIGTLTPLSSIPGWVIGFILILVFAVGLKIL
                     PVGRMIDSIPPESSFEYVLTVIRHMVLPVTAILLSSAFTLIFSWRTYFLAYSHEDYVD
                     VGKAMGLPQKTFQRNYILRPVLPAIITSFSLSMVGFWQLITTVEFVFDWPGIGSLFVR
                     ALPHFLGERMYPGEIPIIIGTMVIFAYLLGFTVFMLDIAYLVTDPRIRVTSGGSSQRL
                     VAKRTKRKWTFPTFKRPEFSWTETKQNFSDTKAQWAENWKKFLALLSELRGYPSAIIG
                     FSIVGLLVLTSIYTVIALPYRQIGRDWFLLSVKPRSVVPKLALPSWVNFFRENDLPST
                     ILIDSRTDSVERTETVLNETTKTITLTYTFDYPYTEFPKDMVIYYYPTYQEKVPFASM
                     ILTTPDGRELELRGEMTSFEKNYEPGSVLNINKILKQNPNWKEWFSIDEHYPTPAFYL
                     LFADPNAKTAKAVPGTYILTINAVGFEKDWDLDAQLVIFGQVYGMAGTDYMRRDLMTP
                     LLWGMPIALIFGLLGSIVITVGSAIVGAAAAWYGGWVDKALQVVIEANMILPVLAVGI
                     LLYAFYQVNFWLILMIIILLNIFGSPTKTFRAAFLQVKEASYVEAARAYGATNWRIIW
                     HYLIPRITPVMVPQLIALIPSFAFLEATFAIFNVSEVMYPTWGLVIQNAMKYGAAYGS
                     QFWVLEPISLLLLTGVAFGMSSAAFDYILNPRLRTKG"
     gene            complement(2850..3443)
                     /locus_tag="PLT08_00515"
     CDS             complement(2850..3443)
                     /locus_tag="PLT08_00515"
                     /inference="COORDINATES: protein motif:HMM:NF017959.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF998 domain-containing protein"
                     /protein_id="HNE02970.1"
                     /translation="MKKLILIGPVLFAVVLVTLTFVKYDFLLSLGWHPINDPTFDWPS
                     GLALGRFGWIMTATFITSGLIMTLFARRLFLDLKPGTASKLGTTLMAFAGLFLATLAF
                     TTDPTIRDTPATWHGQLHDLSFVLLGLTLFPAMIVLGFAFRADERWKNLAVYTWGTLA
                     LAGPAFFIKGAAFYVFLFAILAWNEIAAIRLNSHAEN"
     gene            complement(3492..3890)
                     /locus_tag="PLT08_00520"
     CDS             complement(3492..3890)
                     /locus_tag="PLT08_00520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE02971.1"
                     /translation="MQILFLIHSHMRWLILIVALVAIVKFAIGWLRGGAFKGMDRGLA
                     SGFSGLMDLQATLGLIFLIWTGLAGVGFPMNRIEHATTMIIAAVLAHLPARWKNASDS
                     IRFRNTLFCIVGSLVLVFMGVVRLRGGWTW"
     gene            4032..4805
                     /locus_tag="PLT08_00525"
     CDS             4032..4805
                     /locus_tag="PLT08_00525"
                     /inference="COORDINATES: protein motif:HMM:NF012596.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HNE02972.1"
                     /translation="MTYTTILTETRGRVGIVQLNRPQAMNAFNLAMLGEVFDALEVFD
                     KDENVGAIIVTGNEKAFAAGADIKEMADQTYVQMLANEHVKIWDRIRGIRKPVIAAVS
                     GWALGGGSEFVLSCDMVIASESAKFGQPEITIGIIPGAGGTQRLARLVGKHLAMEMVI
                     NNRTLTAQEAQQFGLANRVVPVEGYLDAAVAFAEEIASRAPIAVRMAKDAVNAAFETT
                     LTEGLAVEKRNFYPLFATEDQKEGMQAFIEKRKPNWKGK"
     gene            4886..5635
                     /locus_tag="PLT08_00530"
     CDS             4886..5635
                     /locus_tag="PLT08_00530"
                     /inference="COORDINATES: protein motif:HMM:NF014571.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046353 - aminoglycoside
                     3-N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAC(3) family N-acetyltransferase"
                     /protein_id="HNE02973.1"
                     /translation="MPASTLNLKSALSDLSLKKRLVLVHASPLVNADSLFTSLLETTS
                     GFFTPTFTPKSLLPLEYHPVHSKADVLSLAQPFTSSTPTDEAFGSFPELVRKHAKGKR
                     SIHPVFSFAGINADAILHSQTQDNLYAPIATLADEDGVILLVGMDQRVNFSIHYGEKL
                     AGRMQFIRWALTEDGVVECPNFPGDSEGFNAISLTVKPFTRQAEVDGITLQAIPVNDL
                     LKSVVNKIHEDAYSLLCGRPDCERCEAVRWG"
     gene            complement(5660..7324)
                     /locus_tag="PLT08_00535"
     CDS             complement(5660..7324)
                     /locus_tag="PLT08_00535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE02974.1"
                     /translation="MNKSLKKVLLFYAILFGVIYAPLILWFGTKSVLAPFELFTSDTF
                     YYLSIARHSVDSSFYTSDGIYPTNGFHPLWGFLLTKLFSLPTFAAHLEPQIYLTIILS
                     LTFTAFGMAFFGMVMYHLTDNAAISLLAAVPGYYYLIFAPLVPNYNSTWAYINGMESP
                     LSIFWFGLLLYLLINKNLLFDQRLWLTVILSIIITIIIFSRLDDVFLLPSFLILTFLF
                     ADTRKQAVARLLITIGIPILSLFVYMAYNYSYAGSAMPISGLIKNGNWLPVNLTFFIT
                     SFFPIQLVNYDPIWNEASMRSLQTVIPVLVAVLWIWFWRKAVKPQNWKSVSRDSNTVI
                     TALSVYVIIKGGYNFFLVYLMGQGHWYYSISIMIFNLMVGVVIANFLRSVSSQSQRVW
                     MGIVSLLFVVLMGNTFVNNKIQSRYNVGYYELWLQREKVNSSLKGLNPDLKLVEYDDG
                     IIAYLLNFPVMNGFGFTLDKEAVQAQTEGRLLNLAYERGFQTIAVMSYINFPSDFEND
                     SDQIRDELSEMPGIGLENLDQWDFEFLYRDDKSSIVLINYEPRTGQ"
     gene            complement(7330..8115)
                     /locus_tag="PLT08_00540"
     CDS             complement(7330..8115)
                     /locus_tag="PLT08_00540"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HNE02975.1"
                     /translation="MKLEVITRIPKEQKYKTPLLFVHGMWHGAWCWDEFFLPFFADAG
                     YHVTALSLRGHAGSEGKIQGSSIMDYVRDVEEVARTLPTPPVLIGHSMGGFIVQKFLE
                     RNTAPVGVLLAPAPPYGVWNGTWQVFKHSPLTLMKILTRFRLHPVVETPESTRWAFFS
                     ADMPQDQLLKYHAKMNDESFRMYLDLLGLNLVRTKRVKTPLLVLGAKEDTVISNHDVE
                     KTAQAYNTKAELFPSMAHDMMLEKGWKDVANRILDWLKEKGIE"
     gene            complement(8188..8259)
                     /locus_tag="PLT08_00545"
     tRNA            complement(8188..8259)
                     /locus_tag="PLT08_00545"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(8225..8227),aa:Lys,seq:ttt)
     gene            8357..8680
                     /gene="trxA"
                     /locus_tag="PLT08_00550"
     CDS             8357..8680
                     /gene="trxA"
                     /locus_tag="PLT08_00550"
                     /inference="COORDINATES: protein motif:HMM:NF012314.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HNE02976.1"
                     /translation="MSSVKYVTEQDFQDEVISSQTPVLVDFTADWCQPCKMIAPIVEQ
                     LAGQWDGKVKVVKLDADQNPNIMMQYGVMGIPTLMLFKGGDVKERITGYLPKDKIEAK
                     VTPHV"
     gene            8734..>9913
                     /locus_tag="PLT08_00555"
     CDS             8734..>9913
                     /locus_tag="PLT08_00555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE02977.1"
                     /translation="MSEQQLIQAGISALQSNDKQKAFSIFAQVVQQYPQSERGWFLLG
                     MSTDDVERRKYCLQKVLSINPNHADAKKQLTLLTPPQPAPPTPTFTSFNRSDFQQQHV
                     PAFVDNEPDATSEFERFSEPLNQPEVQQEPQHQPTPKQTKQIRRKKNNNVILIASISG
                     LCILAFTAMAGFYLLMNSRTTPAIQAANPTQPISATNTTLAMAPVATDTPIPPTPLPT
                     GLPTQPYTPRFEEAACQFDVPPGVNVSCGYAIVPENRTINSGRTIRLAVAVFHSQSSN
                     PKPDPVIFLQGGPGGEAVSLSANAYPILVDPFLSDRDYIAYDQRGTGLSEPALICDEL
                     ERANRQDVYGTIDSGSRELVYQNAFLSCAGLLQTQGVDLSAYNTVENAADLRDIVTLL
                     G"
BASE COUNT         2778 a         2502 c         2154 g         2479 t
ORIGIN      
        1 tcaagatgca tagtcggaat tgtacgccac ataaaatcaa aatttaaaac catactacgg
       61 tggggggagc attttcctag taaatgtata atctcaaagt agaacttgcc tagaccaaga
      121 ttaaggaaac cgaatgcttt ctgaaaattc agagcttgcc catcagggaa gtacagaggt
      181 acaaagagca cgcaaaacag ctcggagtac gttcttcacc gccctgaagt tcatggcgac
      241 aagattgtta acggtccttc tcaccattgg gttgggcaca ttcattacca ttgtggctgc
      301 caatggtggc ggtgccatag acaacgcaga aaagagtcgc attaatgccg tcaccttgcg
      361 gagtgtggac cctttcgcca catctgccga agagcttact aaactacgag cagatatgga
      421 agaaaaagaa gggttaaatc tcccctttct gccgcgccac atgtactgga cgtataaagc
      481 actgactctg aactggggag aagtaaatga cagagtgaag tttcatccgc gctacacaac
      541 ttccaatatg aatgcagaag atagccgcgt gatcctgatg caataccttc ctaacaccat
      601 ccttttgatc ggtgctggct tcttctttct ttttattgca gggattccat ttgcgctaca
      661 cctatctcaa aaccccggtg ggcggttaga caaactcata gggacgctca cgcctctttc
      721 ctccattccg ggctgggtca ttggtttcat tttgattcta gtcttcgctg tgggcttaaa
      781 gatcctccct gtcgggcgga tgatagacag cattccacct gaatcatcct ttgaatacgt
      841 gctcaccgtt atcagacaca tggtcttacc ggttacagcc attttgcttt ccagcgcatt
      901 cacattgatc ttttcctggc gtacctactt cctcgcttac tctcacgaag attatgtaga
      961 tgtaggaaaa gccatgggtc ttccgcaaaa gactttccaa cgaaattaca tcctgcgccc
     1021 cgtattgccc gccatcatca ccagtttctc cctttccatg gtgggatttt ggcagctgat
     1081 caccactgta gaattcgtct ttgattggcc cggcatcggc tcactattcg tacgcgcctt
     1141 gcctcacttt ttaggtgaaa gaatgtaccc cggtgaaatc cccatcatca tcggcacgat
     1201 ggtcattttt gcctacttgt tgggcttcac cgtctttatg ttggatattg cctatctagt
     1261 cacagatcca cgtatccgcg tcacatctgg tggaagctca caaaggctgg ttgccaaacg
     1321 caccaagcgt aagtggacat tcccaacctt caaacgacca gagttctcat ggacagaaac
     1381 aaaacaaaat ttctccgaca caaaagcgca atgggcagag aattggaaga aattccttgc
     1441 gctcttaagt gaactacgcg gatatccctc tgcgatcatt ggctttagca tcgttggtct
     1501 tctggtattg acctccatct acactgtcat cgccttgccc tatcgtcaga tcggcagaga
     1561 ttggttctta ttaagcgtca aaccgagaag tgtggttccc aaactggctt tgccaagttg
     1621 ggtcaatttc tttcgcgaaa acgacctgcc atccactatt ctcatagata gccgcaccga
     1681 ttctgtcgaa cgcacagaaa ccgtactcaa cgaaacgacc aaaaccatca cacttaccta
     1741 cacctttgat tatccttaca cagaatttcc aaaagatatg gtcatttatt attacccgac
     1801 ctatcaagag aaagttccct ttgcatcgat gatcctcacc acacccgatg ggcgcgaatt
     1861 ggaacttcgt ggcgagatga cgtcctttga aaagaattac gaaccgggca gtgtgctcaa
     1921 tatcaataaa atcctcaagc aaaacccaaa ctggaaagaa tggttctcga tcgatgagca
     1981 ttaccccaca cctgctttct atttattatt tgcagacccc aatgccaaaa ctgccaaggc
     2041 agtacccggc acatacatcc taaccatcaa tgcggttggc ttcgaaaaag actgggacct
     2101 cgatgcccag ttggtgatct ttggacaagt gtatggcatg gctggtaccg attacatgcg
     2161 ccgtgatctg atgactccgc ttttgtgggg catgcccatt gccctcatct ttgggttgct
     2221 aggttccatc gtcatcacgg ttgggtctgc tattgtaggc gcagccgccg cttggtatgg
     2281 cggctgggta gataaagccc tgcaagtggt gatcgaagcc aatatgattc tgccggttct
     2341 ggcagttggc attttgttat acgcctttta ccaggtcaac ttttggttga tcttaatgat
     2401 catcatcttg ctaaatattt tcggcagccc caccaaaacc ttccgcgctg cgtttttaca
     2461 agtcaaagaa gcttcgtacg tagaagccgc gcgtgcatat ggcgcaacca attggcgcat
     2521 catttggcac tacctcatcc cgcgcatcac accggtgatg gtgccgcaac ttattgctct
     2581 cattcctagt ttcgcttttc tcgaagccac cttcgccatc ttcaacgttt ctgaagtaat
     2641 gtaccccaca tggggcttgg tcattcaaaa tgcgatgaaa tacggcgccg cctacggctc
     2701 acaattctgg gtcttggaac caatctcttt gctcttattg accggtgttg catttggaat
     2761 gtctagcgcg gcatttgact atattctcaa cccgcgcctc agaacaaagg gataagttga
     2821 ttcagtttct ctggcttttc gttacgctgt tagttttctg catgtgagtt caaacgaatt
     2881 gccgcaattt cattccatgc caaaattgcg aatagaaaaa catagaaagc tgcgcctttg
     2941 ataaagaatg ctggtcctgc caaggcaagc gtcccccacg tatagacggc aagattcttc
     3001 caacgctcat cggcgcggaa ggcaaagcca aggacgatca tcgcggggaa aagcgttagt
     3061 ccaagcaaca caaacgagag gtcatgcagc tgtccatgcc aagtggcggg agtatcacga
     3121 atggttggat cggtggtgaa agccaaggtg gcgagaaata aaccagcgaa cgccatcagc
     3181 gtggtgccaa gtttggaagc tgtgcccggc ttcaagtcca gaaagaggcg acgggcgaaa
     3241 agagtcataa tgagtccgct ggtgatgaag gtggcggtca tgatccaccc gaagcgaccg
     3301 agcgccagcc cactgggcca gtcgaaggtg gggtcgttga tgggatgcca gcccaaactc
     3361 aaaagaaaat cgtatttgac gaatgtcagc gtaacaagca caactgcaaa aagaacggga
     3421 ccaataagaa ttaatttttt catgtttaaa caaaacagcc tcgcatatgc gaggctgttt
     3481 ccttttttag attaccaggt ccaaccgccg cgcaagcgga cgacacccat gaagaccaac
     3541 accaacgaac cgacaataca gaacaaggta ttgcggaagc ggatcgaatc tgaggcgttc
     3601 ttccaacggg cgggcaaatg agcaagcact gccgcaatga tcatggtggt ggcatgttcg
     3661 atgcgattca tggggaagcc aacgcctgcc aagccggtcc agatcaagaa aatcaagccg
     3721 agcgtggctt gtaagtccat cagcccgcta aaaccagaag ccagaccgcg gtccatgcct
     3781 ttgaaggcgc cgccacgcaa ccagccaatg gcaaatttga cgatggcaac caacgccacg
     3841 atcaagatca accagcgcat gtgggaatga atgaggaata gaatttgcat gagagtctcc
     3901 gtatataaat ttagttcaga tatttgggat gaagcggata tagccgttct tacgctgccc
     3961 aatagggttt gagaattata cccagtgcag gttgagttta taatctcact atctgaactt
     4021 tggaggaacc catgacctac accaccattc tgaccgaaac ccgcggacgc gtaggcattg
     4081 tgcagttgaa ccgcccacaa gccatgaacg cgttcaacct tgccatgctt ggcgaagtct
     4141 ttgatgcatt ggaagttttt gataaagatg aaaatgttgg cgcgatcatc gtcaccggca
     4201 atgagaaagc cttcgctgcc ggagccgata tcaaagaaat ggcagatcaa acctatgtgc
     4261 aaatgcttgc caatgaacat gtaaagattt gggatcgcat tcgcggcatt cgtaaaccgg
     4321 tcattgcagc agtttcgggt tgggcgctcg gcggcggaag cgaattcgtg ctttcctgcg
     4381 atatggtcat cgcgagcgag tctgcaaagt tcggtcagcc tgaaattacc attggcatta
     4441 tccccggcgc aggcggcaca cagcgactgg ctcgtctcgt gggcaaacat ctcgccatgg
     4501 aaatggtcat caacaaccgc accctcaccg cgcaagaagc tcagcaattc ggtctcgcga
     4561 atcgtgttgt gcctgttgaa gggtatctcg atgccgctgt tgcctttgcc gaagaaattg
     4621 cttcgcgcgc ccccatcgcc gtccgcatgg cgaaagatgc cgtcaatgcc gcatttgaaa
     4681 ccacgcttac cgaaggtcta gccgtcgaga agcgtaactt ctatccatta tttgcgaccg
     4741 aagatcaaaa ggaaggcatg caagccttca ttgaaaaacg caaaccgaat tggaaaggta
     4801 agtagccttg tagttttttc gtcgagtagt cgcttgatcg atttggacta ctcgacatgc
     4861 aaccatttga ctattcgact aacctatgcc cgcctccact cttaacctca aatctgctct
     4921 ctccgaccta agcctgaaaa aacgccttgt gctggtgcac gcctccccac tcgtcaatgc
     4981 ggactcgttg tttacctccc tgctggaaac caccagtgga ttcttcaccc caaccttcac
     5041 gcccaaatct ctgttgccgt tagagtatca ccccgttcac tccaaagcgg acgtactttc
     5101 attagcacaa cctttcacat cctccacgcc tacagacgaa gcctttggct ctttccctga
     5161 actggtccgc aagcatgcca aagggaaacg ctccattcat cctgtctttt cttttgcagg
     5221 cattaatgca gatgcaatcc ttcattctca aactcaggat aatctttacg cacccatcgc
     5281 cactttagca gatgaagatg gagtcatcct gctcgtcggc atggaccaac gcgtcaactt
     5341 cagcattcat tatggagaga aacttgcggg gcgcatgcaa ttcatccgct gggcactgac
     5401 cgaagatggc gtagttgaat gcccaaactt tcccggcgat tcagaaggct tcaatgccat
     5461 cagtctcacc gtcaagccct tcacccgtca agcagaggtg gatggcatta cccttcaagc
     5521 cattccagtc aatgacctgc tcaagtctgt ggtcaataaa atccacgaag atgcctattc
     5581 tctcctatgt ggtcgccccg attgtgagcg ctgtgaagcc gttcggtggg gataatttac
     5641 gaccttaaga ctttgccttt tattgccctg ttcgtggttc ataattaatt aagacgattg
     5701 aagatttatc gtcacggtat aagaattcaa aatcccattg gtcaagattt tcgagaccaa
     5761 ttcctggcat ctcgctcaat tcatctcgta tctgatctga atcattttcg aaatcagatg
     5821 gaaagtttat gtagctcata acagcaatcg tctgaaaacc cctttcatat gctaggttca
     5881 ataaacgtcc ttctgtttgc gcctgtacag cttctttatc tagtgtaaaa ccaaaaccat
     5941 tcatgacagg gaaatttaac aagtaagcaa tgatgccatc atcatattcc acaagtttga
     6001 ggtctgggtt taatcctttc aaagaagaat taactttttc tctttgaagc cagagttcgt
     6061 aatagccaac attgtatcta ctttgtattt tattattaac aaatgtatta cccatcaaaa
     6121 caacaaaaag taaagaaaca atacccatcc atactctttg gctttgcgag gaaacgctgc
     6181 gaagaaaatt tgcaatcaca acaccaacca tcaggttaaa tatcataata ctaatagagt
     6241 aataccaatg tccctgtccc ataaggtaga caagaaaaaa gttgtatccg cctttaataa
     6301 tcacatatac agacaaagca gtaatcactg tattactgtc tcgcgatacg gatttccaat
     6361 tttgaggctt gacagctttt cgccaaaacc aaatccataa aactgctacc aatactggaa
     6421 taactgtttg taaagaacgc atagaagctt cattccaaat cggatcataa tttacaagct
     6481 ggatcgggaa aaaggaagtt ataaaaaaag tgagattaac tggcagccaa ttcccattct
     6541 ttattaatcc acttattggc attgcactcc cggcatacga gtagttatat gccatgtata
     6601 caaataaaga caaaatcggt ataccaatgg taattaacaa tcgggcaaca gcctgttttc
     6661 gtgtgtctgc aaaaagaaag gttaatatca aaaaagaggg tagcaaaaag acatcatcca
     6721 gccgcgaaaa aattattatg gttatgataa tggaaagaat cacagtcaac cacagccgct
     6781 gatcaaaaag tagatttttg tttatgagta ggtaaagtaa caatccgaac caaaaaatac
     6841 tcaatggcga ctccatacca ttgatgtatg cccaagtact gttgtagttt ggaacaagcg
     6901 gtgcaaagat tagatagtaa tatccaggaa ctgcagctaa caaagaaata gctgcattat
     6961 cagtcaaatg atacatgacc atcccaaaga aggccatccc aaatgctgta aaagtgaggc
     7021 ttaagataat ggtcaggtaa atttgaggtt ccaggtgagc agcaaaggtt ggaagagaga
     7081 acaattttgt gagaagaaaa ccccataatg gatgaaatcc gtttgttgga tagattccat
     7141 cagatgtgta aaatgaacta tctacggaat gacgagcaat ggataaataa taaaaagtat
     7201 ctgaggtaaa tagttcaaac ggggcaagta cacttttagt accaaaccac aaaatcaagg
     7261 gggcatatat aacgccaaac agaattgcat aaaaaagcaa aaccttctta agagatttat
     7321 tcatagaatt tactcgattc ctttttcctt caaccaatcc aaaattctat tcgctacatc
     7381 tttccaaccc ttttcaagca tcatatcatg cgccatgctg ggaaatagtt ctgctttggt
     7441 gttgtaggct tgggcggtct tttctacatc atgattagag attactgtgt cctcttttgc
     7501 gccaaggaca agtaacggcg ttttcaccct tttcgtgcgg accaagttca accccagcaa
     7561 gtctaaatac atacgaaacg actcgtcgtt cattttggcg tgatacttta gaagttggtc
     7621 ttgtggcata tctgccgaga aaaatgccca acgtgtgctt tcaggcgtct caacgacagg
     7681 atgtaaacga aatcgggtga ggattttcat caacgtaagc ggactatgtt tgaatacctg
     7741 ccacgtccca ttccaaactc cgtacggcgg agccggagcc agcaaaacac ccaccggggc
     7801 tgtatttcgt tcaagaaatt tctggacgat gaacccgccc atggagtgac cgatcagcac
     7861 aggcggagtt ggaagcgtcc gtgcaacctc ttccacatct cgaacataat ccatgatact
     7921 actgccttga atcttcccct cactgccagc atgtccacgc aggctgagcg ccgtgacgtg
     7981 ataccccgca tcggcaaaaa agggcaaaaa gaactcgtcc caacaccagg ctccatgcca
     8041 catgccatgg acgaaaagca gcggggtctt atatttttgt tctttaggaa ttcgagtgat
     8101 cacttcaagt ttcatggcaa actctcctct cccctttgag tttactgtat aaaacaaaaa
     8161 tccctcacat aggtaaggga ttttctgtga gcgcggaggg gatcgaaccc tcaacaaacg
     8221 gcttaaaagg ccgctgctct gccattgagc tacgcgccca tctttaaagc ggattgcatt
     8281 ctatcacggc agcatactca cgtcaacgga agtaattaaa atagagaaca tctcatccaa
     8341 gacctcggag gcaaccatga gttcagtcaa atatgtaaca gaacaagact ttcaagacga
     8401 agtcattagc tcgcaaaccc ctgtattggt agatttcacc gcagattggt gtcagccatg
     8461 taagatgatc gcacccattg tcgaacagct cgccggtcaa tgggatggca aggtcaaggt
     8521 cgtcaaactt gatgcggatc aaaaccccaa catcatgatg cagtacggcg tgatgggcat
     8581 ccccaccctg atgcttttca aaggcggaga tgttaaagaa cgcattacgg gctatctacc
     8641 caaagacaag atcgaagcca aagtcacccc ccacgtgtaa gagtataatg gagccaacat
     8701 agtcggctcc attttctttt atatagggaa aatatgtcag aacaacaact tattcaggcg
     8761 ggaatttctg cactacaaag caatgataaa caaaaggcat tttcgatctt tgcccaagtt
     8821 gtacaacagt accctcaatc tgagcgtggc tggtttctgc ttggcatgag caccgatgat
     8881 gtggaaagac ggaagtattg ccttcaaaaa gttctaagca tcaaccccaa tcatgcggat
     8941 gccaaaaagc agcttacgct tctcaccccg ccccagcctg ctccaccaac cccaacattc
     9001 acttctttca accgctcaga cttccaacag caacacgtcc ccgcttttgt tgacaatgag
     9061 ccagacgcca cttcggaatt tgagcgattc tcagaaccgc tgaatcagcc cgaggtccag
     9121 caagagccgc agcaccaacc tactccaaaa caaaccaagc aaatacgtcg caagaagaat
     9181 aacaacgtca tcctgatcgc atccattagc ggactttgca ttctggcctt taccgccatg
     9241 gctggctttt atttgttgat gaacagccgt acaacaccgg caattcaagc agcaaacccg
     9301 acacagccca tttcagccac gaacacaacc ttggcaatgg ctcctgttgc gacggatacg
     9361 cccatcccac ctacaccact tcccaccggc ttaccaacac agccatacac acctcgcttt
     9421 gaagaagcag cttgtcaatt cgatgtgccg cccggggtga acgtttcttg tggttatgcc
     9481 attgtgcctg aaaaccgcac tatcaacagc gggcggacca tccgcttggc ggttgctgta
     9541 tttcatagcc aaagttccaa ccccaaacca gacccggtta tctttttaca gggtggtccg
     9601 ggtggcgaag cggtttcgct cagcgcaaat gcctacccaa tcttagtgga tccatttttg
     9661 agtgaccgag attacatcgc ttatgaccaa cgcggcaccg ggctttcgga accagccttg
     9721 atctgcgatg agcttgaacg agccaatcga caagatgttt acggtactat cgatagcggt
     9781 tccagagagt tggtgtatca gaacgccttc ctctcttgtg ccgggctttt acaaactcaa
     9841 ggcgttgacc tcagcgcgta taacacggtt gaaaatgcag ccgacttaag agatatcgtc
     9901 acattactgg gat
//
LOCUS       DAOQJE010000012         9905 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_3595_length_9905_cov_6.367919, whole genome shotgun sequence.
ACCESSION   DAOQJE010000012 DAOQJE010000000
VERSION     DAOQJE010000012.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 9905)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9905)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9905
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3595_length_9905_cov_6.367919"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2142
                     /locus_tag="PLT08_00560"
     CDS             <1..2142
                     /locus_tag="PLT08_00560"
                     /inference="COORDINATES: protein motif:HMM:NF024583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HNE02978.1"
                     /translation="PGVVLSVQYRLKMKNGEWGHFEVSGSNMLGIPDIAGIVVNYRNI
                     TERVRTEKAIEESEERYRTIFESAGIPIWEDDYSEFMASIEELKRKGITDFRKYFTEH
                     PEFVERAASMIRITGVNAAAMSLMGATTKSQLIGALDSVLFSQPSNRFIDDVVALTDG
                     RRHIEQESELFTLSGERRDVWVTINLPERAKGYERVLVSTLDITERKQIENALIESQG
                     RLLGIVTTAPNGIITVDANIRIILMNPAAEKIFGYLAEEVLGQSLNMLLPERFREHHA
                     KYIQSYSASGVSHRKLGRYDSLYGIRKNGEEFPMEAFISRYEMEGEKFFTVILQDITV
                     RKQDEDNLRRRAEELQTLVIVSSALRSAVKVDEIIPLVARYSAEIVGGTYSTVYLLDQ
                     SSDALVSPGWYSVRNGEDIKTTTAPLLRNASGNGITGYVASTGNIYITEDLRNDPKAS
                     ILPDEIETLRDAHSGISLPLLSQEMVIGVLHVRLTEKHVFNETEIRLLTAIAEMAGNA
                     LHRTKLYEQTVQQSEELAQAYDNTLSGWARALELRDELTEGHTRRVTELTLELARAMN
                     ISESELVQIRRGAILHDIGKMGIPDAILNKPGPLTAHEIRIMQMHTQYAYEMISFIPF
                     LKPAIDIPYCHHEWWNGNGYPRGLKAEEIPLSARIFAVIDVWDALTSDRPYRAAWSTE
                     NALKYIQDGSEKQFDPKVVDAFLKLIENRKL"
     gene            complement(2113..3429)
                     /gene="lysA"
                     /locus_tag="PLT08_00565"
     CDS             complement(2113..3429)
                     /gene="lysA"
                     /locus_tag="PLT08_00565"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: protein motif:HMM:TIGR01048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008836 - diaminopimelate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HNE02979.1"
                     /translation="MNRLNLFPITTKIKNEKLNLAGHDLSQLAEQYGTPLYLYDRATL
                     DSATSEYKSAIASFYPAPASVTYAGKAFLCLAIAQWTQFHDLTVDCTGEGEIAIAVAA
                     GVSKENILVHGVNKSEQDLKSAIKYASTIVVDNLNELRRIQSLFTADNTTFPTLWLRL
                     LPGVSVTTHHAHTQTGHHESKFGMTYEEILEAAKFCKENNLPLKGIHFHQGSNFRDPE
                     PLLPAIDLALDLAKEIGFTSDWHFCPGGGWGVAYHEDELPSPSIESYVRGISEAVVDG
                     CRMRGLTLPHLHLEPGRSLVAQAGVAMYRVGAIKKRGHKTWLLTDGGMADNPRFALYG
                     ARYSCLTVSGPGGERSERVSIAGPYCESGDIVIEDLPMPKVKEGDLIAIPVAGAYHLS
                     MSSNYNGARKPAVLWLEEDHVQLIRQRETVQDLFEADLKLAVLDKL"
     gene            complement(3422..5953)
                     /locus_tag="PLT08_00570"
     CDS             complement(3422..5953)
                     /locus_tag="PLT08_00570"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:NF028237.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HNE02980.1"
                     /translation="MEPALAVYLFCLAVLVAILLGIAIRAFSHWKTPGTRTLGALILT
                     MAVWAAFYILEVIHPSLEVKILARKMLYFGMTMAPPFWLAFALRYTGLGAWWSQQGRV
                     FLFAIPGGIAFLFGLTNEFHHLIWSDLSLSATKPVLLTIEYGIGFWVYAILAYLQIFV
                     GLAIYLVAHFKNKKAFRTKTGIVLAGLLLTAIVNVLFLLFGNSLNVDPTPLSFALSAP
                     LIAWGFFRFGVSSLFPLAASLVVENLQDAIIIVNREDEITDINQTAAELINLKTVKEK
                     SNIFTLLPFAEQLKPIWDAQKSSIKLETNFRGASQWVEAQVIPIKDHEDSLLGRVIVL
                     HDITKEQALLRAERRRSQQLSLLEQTGRYIAGSFDEQEILQRTVDAITQQFGYPETAI
                     SLLKNNMFEIAAIFGTEDFGYRQGYRQEIGSGIIGYTASIKKTYVSRNVSQDPYYYST
                     STKSGSAICTPIFKQDALYGVLYVESFDLDAFDELDIITLETLASQMSASLQRAELYA
                     QTQNDLLTLRTIQNISKLITSSLDLETISQTVVRSLKESFDYTHVSIYLLEDEYLHLA
                     AEVGYPKELVIYKIHISQGVIGKAYRTRSIQFIEDTTKEKVFLKADNHITSEICIPLL
                     KEDIVLGMLNVESNVSNKLSQRDVTLLTTIAGPIAIAVDNARLHTEIKRMATTDAVTG
                     LSNRHVFEQALLAEFERAERSHSQMSLIIFDIDFFKQYNDIWGHPAGDARLKGVANII
                     KLNLRKYDIAARYGGDEFAIILANCDQQSAMTFAQRLQQGTIAGAPQQSENDAGIPGY
                     TLSIGIATYPQDATLPSELLVAADNAALRAKLHGRNQIKFANDYE"
     gene            complement(5969..6346)
                     /locus_tag="PLT08_00575"
     CDS             complement(5969..6346)
                     /locus_tag="PLT08_00575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE02981.1"
                     /translation="MNQFPIKFRRIAVFAGIFILVLFVIEFNSRLEELNRLNEQRDEM
                     RAQATQAVQTQIALQTQVAYAASTDAVEEWARTDGHYLQEGDQPVIPVEQPGSDPVII
                     ETPIPVPTPMQNWEIWWTLFFDK"
     gene            6468..7001
                     /gene="rplI"
                     /locus_tag="PLT08_00580"
     CDS             6468..7001
                     /gene="rplI"
                     /locus_tag="PLT08_00580"
                     /inference="COORDINATES: protein motif:HMM:TIGR00158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HNE02982.1"
                     /translation="MKVLLIKDVYKLGRAGDIKKVADGYGRNFLLPQGLAVLASEGAM
                     KQVQKIKAQAEIRRSTQNNELKGVADLIKDVTLIFPAKAGDTGKLYGSITTGDIATAL
                     SEKIRFEVKRQQVDTQPIRTLGEFTAHVRLTMDLVPDIKIIVHREGESPESAAGQHEE
                     PAKAEKKEEAAAETKAE"
     gene            7059..8747
                     /locus_tag="PLT08_00585"
     CDS             7059..8747
                     /locus_tag="PLT08_00585"
                     /inference="COORDINATES: protein motif:HMM:NF024855.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4115 domain-containing protein"
                     /protein_id="HNE02983.1"
                     /translation="MQETIGQRLKREREARYLSLEKAAEETRIRRVFLQALEADDYSV
                     MPSAAQGRGFLRNYAEYLNINIDELIAEIQRNPIPLAEVSGPLPQVNLAETELPPLTE
                     AEDEKSAPPFWTRFLNRKPKEESIPEAPQEPSPEIEQILEEQPPAVVEEEKPKTRGRK
                     KKIVVEEAIVEPVAELKVEEPESQPSVESQPDGQEQVEIQEEPKQAWWGRVVSLFKIK
                     VQKQDSAFEVVLSEDKEDIPQNGVSSIPDKPADVIMAEIGKMLRERRELISLTVDEVE
                     RHTHLRAVFVKALEEGAFDKLPSTVQTRGMLSNYASFLDLDVDTVLLRYADALQARRR
                     EKYAETPREKIQMQVNPSMPVLRTFIAGDLLFGVIMIAVIVALAIWGVGRLVSSDQQT
                     QVQPTSPSIVDVLGNLSDATASPAVTFVAVDDPAIANAGTAAEGTVTPAGSEVASTAN
                     VVVNVYALERVFVRISVDGEVAFEGRLAPRESRFFEANDQVVILTGNGSGLRITYNGE
                     DLGLMGGPGEVVNRVYLITGVVTPTATIPPTPTNTPLTTPTLTPTVTPTGTATP"
     gene            8783..>9905
                     /gene="rimO"
                     /locus_tag="PLT08_00590"
     CDS             8783..>9905
                     /gene="rimO"
                     /locus_tag="PLT08_00590"
                     /EC_number="2.8.4.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR00089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008172 - S-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0018339 -
                     peptidyl-L-beta-methylthioaspartic acid biosynthetic
                     process from peptidyl-aspartic acid [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     RimO"
                     /protein_id="HNE02984.1"
                     /translation="MSKNTFHLVSLGCSKNTVDSDSMAQLLTRDGYSFVEDPSKASVL
                     IVNTCGFIGPAKDESYRVLGELAQGKKKNQVLIAAGCLTQRYGVEVAKKVPGIDGIIG
                     TRRWMDIVQVVEDARKGKHPEPIYHLPEAATVGADENGAMRVSVTGPSAYLKIADGCR
                     RPCAFCAIPLIKGTAVSRPVESIIHEARTLRDSGVRELILIAQDSTDYGHDLGMKDGL
                     ATMLEQLTDAVPDMDWIRIMYSYPGYVTDRMIEVMATRKQILPYLDIPLQHAHPKTLY
                     RMKRPSNMDWVHKTLAKMRATIKDLAIRTTFIVGYPGETDEEFQALYDFVEQTRFDRA
                     GAFQFSFEPGTTSAPLGDPVPAEVKQERYERLMELQQNIS"
BASE COUNT         2686 a         2201 c         2384 g         2634 t
ORIGIN      
        1 cctggtgtcg tcctttctgt tcaataccga ttgaaaatga aaaatggtga gtgggggcat
       61 tttgaagtaa gcggaagcaa tatgttgggt attccagata ttgctggaat tgtagttaat
      121 tatcgaaata ttacagaacg ggttcgaact gaaaaagcca tcgaagagtc tgaggaaaga
      181 tatcgcacca tctttgagtc tgctgggatt ccaatttggg aagatgatta ttctgaattt
      241 atggcaagta ttgaagaatt aaaacggaaa ggtatcaccg atttccgtaa atattttact
      301 gaacacccgg aatttgtcga gcgtgctgca agtatgatcc gcattacggg ggttaatgct
      361 gctgcaatga gtttaatggg cgcaaccaca aaatcacaac taattggtgc acttgatagt
      421 gttttatttt ctcagccatc aaacagattt attgacgatg ttgtggcact gacagatgga
      481 cgtagacata ttgagcaaga gagcgaattg tttactttgt ctggcgagcg ccgtgatgtg
      541 tgggtgacca ttaatttgcc tgaacgtgca aaagggtatg aacgagttct tgttagtaca
      601 ttagatatta cagagagaaa acaaattgag aatgctctaa tcgagagcca gggcaggctt
      661 ttgggtattg tgactacagc gcccaatggc attattacag tcgatgccaa tatccgcatt
      721 atcttgatga acccagcggc tgagaaaata tttggctatc tggcagaaga agttttaggg
      781 caaagcctaa atatgttgct tccagaacgt ttccgggaac atcatgcaaa gtatattcaa
      841 tcctatagtg caagcggagt ttcccatcgt aaactcggta gatatgattc gttatatggt
      901 attcgcaaga atggtgaaga atttcccatg gaagccttca tttcacgtta tgaaatggag
      961 ggagagaagt tctttactgt aatcttgcaa gatattactg ttcgcaaaca agacgaagat
     1021 aatttgcgcc gccgagcgga agaattacaa accttggtca ttgtctcttc tgctcttcgt
     1081 tcagccgtaa aagtggatga gattattcca ttggtggcac gctattctgc tgagatcgtt
     1141 ggcggcacct atagcactgt ctatctttta gaccaatctt ccgacgcctt agtctcgccg
     1201 ggatggtact cggtacgaaa tggggaagat atcaagacca ccacagcacc gctattgcgg
     1261 aatgcttctg gaaacggtat aaccgggtat gtagctagca cgggcaatat ttacattact
     1321 gaagatttgc gtaatgaccc aaaggcttct atcttgccag atgaaataga gacgcttcgt
     1381 gatgctcata gtggcatctc actgccactg ctttctcaag aaatggttat tggggttctg
     1441 cacgtgcgtc taacagaaaa gcatgttttc aatgaaaccg aaattcgctt gcttacagcc
     1501 attgcagaaa tggcagggaa tgctcttcat cgcacgaaac tttatgaaca gactgtgcaa
     1561 caatctgaag aactggctca agcatacgac aacactcttt ctggctgggc acgtgcattg
     1621 gaattgcgcg atgagttgac agaggggcat actcgccgcg taaccgaact aacgctggaa
     1681 ctggcacgag ctatgaatat ttcggaaagc gagctggtgc aaattcgccg tggtgccatt
     1741 ttgcatgata ttgggaagat gggtattcca gatgcaattc tcaataaacc aggtccattg
     1801 acagcgcatg aaattcgaat catgcaaatg catacgcaat atgcatacga aatgatctca
     1861 tttatcccgt tcctaaaacc agctatcgac atcccttatt gtcatcatga atggtggaat
     1921 ggcaacgggt atccacgcgg gttgaaagcg gaagagattc cactctctgc ccgaatcttt
     1981 gcagttatcg atgtttggga tgcactaaca tcagaccgtc cataccgcgc ggcttggtct
     2041 acagaaaatg cgttgaaata tatccaagac ggttccgaaa aacagtttga ccctaaagta
     2101 gtagatgcct tcctaaagct tatcgagaac cgcaagcttt agatccgctt caaacaagtc
     2161 ctgaaccgtt tcgcgttgtc gaatcaattg cacgtggtct tcctccagcc ataaaacagc
     2221 aggtttgcgc gcgccgttat aattcgatga catactcaaa tggtatgccc ccgctacagg
     2281 gatcgcaatc aaatctccct ctttgacttt tggcatcggc aaatcttcaa tcacaatatc
     2341 cccagactca caatacggtc ccgcaatgga gacacgctcg cttctttccc cgcccgggcc
     2401 tgataccgtt aaacaagaat atcttgcccc gtaaagtgca aagcgcgggt tatctgccat
     2461 gcctccatct gtaagtagcc aggttttgtg accgcgcttt tttatagccc ccactcgata
     2521 cattgccaca ccagcttgcg caactaggct acgacctggc tcaagatgta aatgggggag
     2581 cgtaagccca cgcattcggc aaccatctac taccgcttca gaaatcccgc ggacataact
     2641 ttcaatcgat ggggaaggta attcatcttc atgatatgcc actccccaac cgccaccggg
     2701 gcaaaaatgc caatcacttg tgaaaccaat ttctttggca agatcaagag ccaaatctat
     2761 ggcaggaagt aagggttctg ggtcacggaa gttagaacct tgatgaaaat ggataccctt
     2821 cagtggcaag ttgttttctt tacaaaattt cgcagcttca aggatctcct cataggtcat
     2881 gccgaatttg ctttcgtgat gtcctgtttg agtatgggca tgatgtgtgg tgacagaaac
     2941 tccgggcaat aggcgaagcc acaatgttgg gaatgtagta ttgtcagcag taaataagct
     3001 ctgaatgcgc ctaagctcat tcaggttatc aacaacgatt gtggatgcat actttattgc
     3061 agactttaga tcttgttcag acttgttaac cccgtgaact aaaatattct cttttgagac
     3121 cccggcggct actgcaatgg caatttcgcc ttcccctgtg caatcgacgg ttaagtcatg
     3181 gaattgagtc cattgtgcaa tggcaaggca gaggaatgct ttccctgcat aggttacaga
     3241 agcaggcgcg gggtagaaag aagcgatcgc cgacttgtat tccgaagtcg ccgaatccaa
     3301 tgtcgcacgg tcatacaaat aaagcggggt cccatattgt tctgcgagct gagaaagatc
     3361 gtgcccggca agattcagtt tttcattctt gattttcgtt gtgatgggga agaggttaag
     3421 tctattcata gtcatttgca aatttgattt ggtttcgtcc atgtagtttg gcgcgcaagg
     3481 cggcgttatc cgcggcgacc aaaagttctg aaggtaaggt tgcatcttgc ggataggtgg
     3541 ctattccaat gctgagtgta tacccgggaa tgcccgcatc gttttctgat tgttgaggtg
     3601 cgcctgctat cgtaccttgc tgcaaacgtt gagcaaatgt catggcactt tgttggtcgc
     3661 aatttgccaa aatgatcgca aattcatcgc cgccatagcg tgcggcaata tcgtatttgc
     3721 gaagatttaa tttgatgatg ttggcaacgc ctttcaggcg ggcatctcca gcggggtgtc
     3781 cccaaatgtc attgtattgt ttgaaaaagt cgatatcaaa aatgataagc gacatttgcg
     3841 aatggcttcg ctcggctctt tcaaattctg cgagcagggc ttgttcaaag acatggcgat
     3901 ttgacagccc agtcacggca tcggtcgttg ccattctttt aatttcagta tgtaaacggg
     3961 cattatctac cgcgattgca atgggaccag caatggttgt caacaaggtt acatcccgtt
     4021 gagataactt gtttgagaca ttcgattcaa cattgagcat cccaagtacg atatcttctt
     4081 ttagtagagg gatacaaatt tcactggtga tgtggttatc tgccttgagg aaaacctttt
     4141 ccttagttgt atcttcaata aattggatag accgggtacg ataagctttc ccaatcactc
     4201 cctgactgat gtgaatttta tagatcacca attctttggg gtagcccact tctgccgcga
     4261 gatgcaagta ctcgtcttca aggagataaa tactaacgtg tgtatagtca aaagactctt
     4321 tcaagctgcg cacaacggtc tgactgatgg tctcaaggtc gagcgaactt gtaatgagct
     4381 ttgaaatgtt ttggatcgtt cgcagagtaa gtaggtcatt ttgggtttgt gcatacagtt
     4441 cggcgcgttg tagtgatgcg gacatctgac ttgcaagggt ttcaagggta ataatatcca
     4501 gctcatcgaa agcatctagg tcaaaacttt ctacatatag aacgccgtag agagcatctt
     4561 gtttgaagat cggggtacag attgccgatc cagactttgt acttgttgaa taataatagg
     4621 ggtcttgcga gacgttccga gatacataag ttttcttaat gctggcggta tacccaatga
     4681 tgcctgagcc aatttcttgt ctgtatccct gacggtagcc gaaatcttct gtgccaaaga
     4741 ttgcggcgat ttcaaacata ttgtttttca ataaagaaat ggcagtttct gggtagccaa
     4801 attgctgtgt aatggcatcc acggttcgtt gaaggatttc ttgctcgtcg aagctgccag
     4861 caatatacct gccggtctgc tccagtaggg ataactgttg ggagcgacgt ctctctgcac
     4921 gcaacagagc ttgctccttg gtaatgtcat gcagaacaat taccctgccc agtaaactat
     4981 cttcatggtc tttaatgggg atgacttggg cttcaaccca ttgtgatgca cctctaaaat
     5041 tcgtttctag cttaatcgag cttttttgcg catcccatat tggtttgagt tgctccgcaa
     5101 aaggcagaag cgtgaaaata tttgattttt cttttacggt ttttaagttt attaattcag
     5161 cagctgtttg gttaatgtca gtaatctcat cttcacggtt tacgatgatg attgcatctt
     5221 gaaggttttc taccaccaac gatgccgcga ggggaaatag acttgaaact ccaaaacgga
     5281 aaaaccccca tgcaatcaat ggtgcagata atgcaaaact gagcggagtt gggtctacgt
     5341 ttaagctgtt tccaaaaaga agaaaaagga cattgacaat ggctgttaaa agtaaaccag
     5401 ctaaaacgat acctgttttt gtacgaaagg cttttttgtt tttgaaatgt gctacaagat
     5461 aaatcgcaag acccacgaaa atctgaaggt aggcaagaat tgcataaacc caaaacccta
     5521 tgccatactc aatagtgagc agaaccggtt tggttgcgga taaagaaagg tctgaccaaa
     5581 tcaaatgatg aaattcgtta gttaagccaa acaggaatgc aattccaccc ggaatggcga
     5641 ataaaaagac acgcccttgt tgtgaccacc acgctccaag ccctgtgtat cgcaatgcga
     5701 atgccaacca aaatggcggt gccatggtca taccgaagta aagcattttc cgcgctagaa
     5761 tttttacttc aagagaagga tgtataactt caagaatata aaatgccgcc caaactgcca
     5821 ttgtcaatat cagtgcccca agggttcggg ttccgggcgt tttccaatgg ctaaaggcgc
     5881 gaatcgctat ccccaataat atagcaacta aaactgctaa acaaaataaa tatactgcta
     5941 gggcaggttc catataggaa agttatggtt acttatcaaa gaaaagtgtc caccagattt
     6001 cccagttttg cattggtgta ggtacgggaa tgggagtttc aatgatgacg ggatcactac
     6061 caggctgttc taccggaata acgggttgat cgccttcttg taggtagtgt ccatcggtgc
     6121 gtgcccactc ttcgacggcg tctgttgatg cagcataagc aacctgggtt tgtaacgcaa
     6181 tctgggtttg gacagcttgc gtagcttggg ctcgcatctc atcacgctgt tcgttcaaac
     6241 gattgagctc ttcgaggcgg gagttaaatt caattacaaa aaggacaaga ataaaaatgc
     6301 ctgcaaagac tgcaattcgc ctaaacttga tagggaactg gttcatgccc attatcttaa
     6361 ccttaatctc tgtcattggc aaaccattca attgtggtaa aatcttgccc cgcgggtcgt
     6421 tgtacccttc gacctgactt caaattttgc cagaaagggc ttaaatcatg aaagtattac
     6481 ttatcaaaga cgtttacaaa ctcggtcgcg ccggtgacat caagaaagtt gccgatggtt
     6541 acggacgcaa cttccttctc cctcagggtc tcgctgttct cgcttctgaa ggagcgatga
     6601 agcaggttca gaaaatcaag gcgcaagctg agattcgccg ctctacccag aacaatgaac
     6661 tcaagggcgt tgctgacctc atcaaagatg taactcttat cttccccgcc aaggctggtg
     6721 ataccggaaa gctttacggt tccatcacca ccggtgatat cgccactgca cttagcgaaa
     6781 agatccgctt tgaagtcaag cgccaacagg tagataccca acccatccgc acgctcggcg
     6841 aattcactgc ccacgttcgc ctcaccatgg acttggttcc tgatatcaag atcatcgtcc
     6901 accgcgaagg tgaaagcccc gaatcagccg ctggtcaaca cgaagaacct gccaaagctg
     6961 agaaaaaaga agaagccgca gcagaaacca aagcggaata acaaaaatgt caaaacatca
     7021 ccggcgcaag tcggtgatgt ttataataca atacttccat gcaagaaacc atcggtcagc
     7081 gattgaagcg cgaacgcgaa gcacgttatc tctcgcttga aaaagcggca gaagaaacac
     7141 gtattcggcg cgtcttcctc caagctctcg aagcagatga ttattccgtc atgccgtctg
     7201 ccgcgcaggg gcgtggtttt ttgcgcaact atgccgagta tctcaatatc aatattgatg
     7261 aactcatcgc tgaaattcaa cgcaacccca ttccactcgc agaagttagt ggaccacttc
     7321 ctcaagtcaa tttagcagag acggaacttc ctcccttaac ggaagcagag gatgaaaaat
     7381 ccgcgcctcc tttctggacc cgcttcctga atcgcaaacc caaagaagag tccataccgg
     7441 aagcgcctca agaaccctct cccgaaattg aacagattct ggaagagcaa cctccagcag
     7501 tagttgaaga agaaaaaccg aaaacacgcg gcaggaagaa aaaaattgtc gttgaagaag
     7561 caatagttga accagttgct gaattaaaag tggaagaacc cgaatcccag ccatctgtgg
     7621 agagtcagcc agatggacag gaacaggtcg aaatccagga agagccgaag caagcttggt
     7681 gggggagagt tgtttcgtta ttcaagataa aagttcaaaa gcaagactcg gcgttcgagg
     7741 ttgtgctttc agaagataag gaagatatac cgcagaacgg tgtttcgtcc attcctgata
     7801 agccggctga tgtcattatg gcagagatcg gtaagatgct gcgcgagcgg cgtgaactca
     7861 tcagcttaac tgtcgatgaa gtggagcgtc atacgcattt gcgtgcggtg tttgtgaaag
     7921 ccttggaaga aggtgctttt gataaactgc cttccaccgt gcagactcgt ggcatgcttt
     7981 caaactatgc cagttttctt gatctggatg tagacactgt ccttttgcgt tatgccgatg
     8041 ccttgcaagc aagacgccgt gaaaaatatg cagagacccc gcgcgaaaaa atccaaatgc
     8101 aagtcaaccc gtctatgccg gtgttgagaa cttttattgc aggcgatttg ctttttggcg
     8161 taataatgat cgcggtcatt gttgctttgg caatttgggg cgttggaagg ctggttagct
     8221 cagaccagca aacacaagtt cagccaacgt ctccatctat tgtggatgtg ttgggcaatt
     8281 tgtctgatgc gacggcttca cctgcagtga cctttgttgc ggtagatgac cctgccattg
     8341 ccaatgctgg cacagccgca gaaggcacag ttactccggc gggttctgag gttgcttcga
     8401 ctgcaaacgt agttgtgaac gtatacgcat tggagcgcgt gttcgttcgt atttctgtag
     8461 atggtgaggt tgcctttgag ggacgtttgg caccgcgcga aagccgtttc tttgaggcaa
     8521 acgaccaggt tgttattctg actgggaatg gttctgggct gagaatcaca tataatggtg
     8581 aagatcttgg tctgatgggc ggacccggcg aagtggtgaa ccgggtttat ctcattaccg
     8641 gtgttgtcac gcctacggcg accatcccgc cgacacccac gaatacgccg cttacaaccc
     8701 caaccttaac gccgactgtc actccaacag gaacggcaac tccgtaattc ttagtttcat
     8761 caatttattg tcaggatcaa acgtgtctaa aaatactttt catttagttt ctctcggttg
     8821 ttcaaaaaat actgtcgact ctgattcgat ggctcaattg ctgacccgcg atgggtatag
     8881 ttttgtcgaa gacccatcga aggcttcggt cttgatcgtt aatacctgcg ggtttattgg
     8941 tccggcaaag gatgagtcat accgtgtgct gggggagttg gcgcaaggca agaaaaagaa
     9001 tcaagtgctt attgcggcag gatgtttgac gcaacgctat ggggtggagg ttgcgaaaaa
     9061 agtgccgggc attgacggta ttatcggcac tcgccgttgg atggacattg tgcaggttgt
     9121 agaagatgct cgtaagggca aacaccctga accaatttat catttgccag aagcagctac
     9181 tgtaggagcc gatgaaaatg gcgcgatgcg cgtcagtgtg acgggaccca gtgcttatct
     9241 gaaaattgcg gatggatgtc gccgcccgtg tgcgttttgt gctattccgc tcattaaggg
     9301 cacggcagtt tcgcgccctg ttgagtccat catccatgaa gcacgcacct taagagatag
     9361 cggtgtgcgt gaacttattt tgattgcgca agactcaact gattatggtc atgacctcgg
     9421 catgaaagat ggtcttgcca ctatgctcga acagttaacc gatgcggtgc cggatatgga
     9481 ttggattcgt atcatgtatt cttacccggg ttatgtcacc gatcgaatga ttgaagtgat
     9541 ggcaactcgt aaacagattt tgccgtactt ggatattccg ttgcaacatg ctcaccctaa
     9601 aacgttgtat cgtatgaagc gcccgtcgaa tatggattgg gttcacaaaa cgcttgccaa
     9661 aatgcgcgca accatcaaag accttgccat tcgtacgacc ttcattgttg gatatcccgg
     9721 tgagacagac gaagaatttc aagccttgta tgattttgtg gaacaaaccc gcttcgaccg
     9781 tgccggagct tttcaattct ccttcgagcc gggtactacc tccgcgcctt taggtgaccc
     9841 agtccctgct gaagtgaagc aggagcgtta tgagcggttg atggaattgc agcagaatat
     9901 ttcgt
//
LOCUS       DAOQJE010000013         9791 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_3665_length_9791_cov_6.643694, whole genome shotgun sequence.
ACCESSION   DAOQJE010000013 DAOQJE010000000
VERSION     DAOQJE010000013.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 9791)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9791)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9791
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3665_length_9791_cov_6.643694"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2827
                     /locus_tag="PLT08_00595"
     CDS             <1..2827
                     /locus_tag="PLT08_00595"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HNE02985.1"
                     /translation="TGNQLYLATTAPALPPEMPDQFRFANLADHPHIQTTLAKGSSVI
                     IPDTEVAELTEAEREICITRGLRSIAYLPLMSARTAIGVLIVASTKKLRTFTDEELLL
                     YHGFSVQAAQTIENAQLYDSARVHSNELEQQISERKQAEKKLQESEKLYRYAMEVAGT
                     VPYYESYSEKHPAVTYEFIGEGIRQITGYGPEEFNSQIWDSLVEKAIPVEELEGYSLD
                     EAIQRTRSGEFPIWKCEFRLHDRDGKTHWVFEAAVELRDANGNPVGSIGSYQDITIRK
                     QAEEKLRKSEEQYRLLFEVASDGVVLHLLSTDRIKNRFRRFNQNICQMLGYTAEELAQ
                     LSPMDIQQPEELLKVPGEAEQLERNRELRFEKVFRRKDGSVFPSEVHSSVFELDGELL
                     VLSIIRDISERKQAEAKLRESEEKYRGLMEAIDSVVATIDYNGRFLYLNEVAAKWLGG
                     TPQELVGKTMFDLFPEPMALQQMEGIREVFQDNQSKVSESLTYIQGQYRWFHNSLQPI
                     HNELGEVVNVLLHAVDVHELKVVQQELADLNRSLEQRIEERTIEVRQTNLALERALLA
                     KDEFLANMSHELRTPLNGILGMAEILLEEEYGLLNERQHAYVNLIDSSGHHLLSLIND
                     VLDLSKVEVGKLELDTEKVFIEDLCQSSMVFVRQLAKKKNVLVNYLPDPAISTLQADA
                     RRLKQILVNLLSNAVKFTPEGGKVMLEVHANRDEGIVTFSVTDTGIGISEDNQKRLFQ
                     PFTQVDSSLSRKYDGTGLGLALVKRLTELHGGTVALQSEVGHGSCFTVSLPWREAGST
                     SHKLDGADAGDNHKFAPSAQTIHATVLLVEDNTYNVMAISEYLEAKGYRLVFAVDGLD
                     ALAKAAENSPDLILMDIQMPNMDGLEATRRLRSDSAFASIPIIALTAHAMGGDRERCL
                     AAGATDYLSKPVKLKELESTIRKWLSS"
     gene            complement(2824..3633)
                     /locus_tag="PLT08_00600"
     CDS             complement(2824..3633)
                     /locus_tag="PLT08_00600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE02986.1"
                     /translation="MKSDVIFPLTRIIAGIVVLFLVAAFGILFFLPDQTGTLFAWAIK
                     PHMSSMFFGSAYLGGAWILAQTAFGPRWHRVQAVFPAVTIFTTAMLAATLPHWERFSL
                     GTIPFLAWLILYIVSPFLIPALWIYNRNTDSHEPEESDVTVPTTARLILRLFGGLVLL
                     LVTICYLIPSLIIPIWPWELTPLTARVLCGWLSVIGTLSLVNSFDPRWTSWRASFEGI
                     FIAYSLILVAATMNPAEFKTGIINWYTLFTAGMLIAIVIFYGIMESRRRKR"
     gene            complement(3661..4089)
                     /locus_tag="PLT08_00605"
     CDS             complement(3661..4089)
                     /locus_tag="PLT08_00605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3592 domain-containing protein"
                     /protein_id="HNE02987.1"
                     /translation="MQPSDIPNEKTAKRIGNQASFIILALVFSCVVSFFFLFNVDKIK
                     MLQTSIDLQFKGSTTTGTVIDLEEFKSSAGHNAVFILTAEFEVDGQPYTVTGNAYYPT
                     LDHSWQGESLDVIYDPQDPNIAMINTFEERWVIPLAGALP"
     gene            4267..5079
                     /locus_tag="PLT08_00610"
     CDS             4267..5079
                     /locus_tag="PLT08_00610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE02988.1"
                     /translation="MMKRNQPILMFVAVLTIAALACNLPTTSNPADATEEPTEVSTEA
                     PEATQTEAPTEEASDSVSVSVSTATNCRTGPDVVYQLLMTVQPGSDFDVVGKYTPKGY
                     WIINMPTGGTCWLWGQYAIVQGDTSALPEITPPPAPVAQPTSETSNQANNSNTNNNEE
                     EDPQPSAPAPAAPTNTDITRQCTTLTQPGDLFPSFKMVVQFTWNDASDNETGFNIYKD
                     GTFYTTLPANTQKFTETFTVLLAQSPSITYGVQSVNANGSSGLVTGTVSYCK"
     gene            5147..5413
                     /locus_tag="PLT08_00615"
     CDS             5147..5413
                     /locus_tag="PLT08_00615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE02989.1"
                     /translation="MAEMTCPKCSGAMEEGLQFNFKLNELSDDYVIETAELTRDRWQK
                     TEDATGKFFGKEYLGKRRVGPRLAVITYRCTVCGYLESYAPPVK"
     gene            complement(5459..6124)
                     /locus_tag="PLT08_00620"
     CDS             complement(5459..6124)
                     /locus_tag="PLT08_00620"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HNE02990.1"
                     /translation="MERILEPELMEDMEQALAYSHADFATSHQMRVTWFQERFGKDNQ
                     PASILDLCCGGGDMTFRFARAFSHSRIIAVDGSQAMLDIAQKDVQAEPEIANRIQFVR
                     AFLPSDEIPQNKYDLVMSHSALHHFHDPNALWKTIRAHTRPGTLVFISDLQRTESVEA
                     AEQLVRERAGNEHPILQKDFAASLCAAFTPEEVREQLNANGLDALKVEAINDVYMLVY
                     GMM"
     gene            complement(6145..7152)
                     /locus_tag="PLT08_00625"
     CDS             complement(6145..7152)
                     /locus_tag="PLT08_00625"
                     /inference="COORDINATES: protein motif:HMM:NF013811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HNE02991.1"
                     /translation="MSRIRLIVILCLTALACQLPSIPILATQTPTQTPTATVTSTPTQ
                     TLTPEPTLTPSITPTPTPLARRVLILSIDGFRPDAIPLAPMPFLTSLLSQSAYSLTAQ
                     TVYPSVTLVSHTSMLSGQCPAKHKVDWNDYIPENGYAQVTDLFDIVHAAGMQTVMYVG
                     KEKLRQITEPQSTDIFKFINDRDLVITDNLIENFPTDFRLMFVHFPTTDWMGHEYGWL
                     SGEQLSVLFRADQALEKLVTELNNRGLMSETLFIITADHGGHATTHGTSLYEDMTIPW
                     IAFGAGIKPAALNSSITTVDTAATAAFALGLNIPTEWDGVPVYEAFGLPIEKASVGCS
                     K"
     gene            7194..7934
                     /locus_tag="PLT08_00630"
     CDS             7194..7934
                     /locus_tag="PLT08_00630"
                     /inference="COORDINATES: protein motif:HMM:NF014090.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VIT1/CCC1 transporter family protein"
                     /protein_id="HNE02992.1"
                     /translation="MDTETSRTLHEELIHHNNVDPHKRGSGLSDFILGAQDGLVNVLG
                     VVLGIAAATNDARVVLVAGLATTFAESISMGAVAFTTTLADADLYQSEREREYRHIAE
                     APMLETKEIRDIYASKGFTGDLLDRIVETITANKDVWVAVMMAEEHQLSPVDRGTAMK
                     AAWVVGLSAIIGSLVPILPFIFLPVSISMWLSVLVTALVLFGIGFYKARVTVGKPMRS
                     GTEMMLIGTISAMAGYLVGVVLKVPPLP"
     gene            complement(7863..8255)
                     /locus_tag="PLT08_00635"
     CDS             complement(7863..8255)
                     /locus_tag="PLT08_00635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE02993.1"
                     /translation="MKLVLIYTLIAGVISAITAPIPGTSLLLTALEVYMIVHLSKSNE
                     SKLGFKEIGYSAMALYGLSTVLKDAALEILTFVPLIGWGAEVIVAMLFVFFLGMLANL
                     YFSKPSRKGRNLENNPDEISSHSTDRPN"
     gene            complement(8252..8743)
                     /locus_tag="PLT08_00640"
     CDS             complement(8252..8743)
                     /locus_tag="PLT08_00640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE02994.1"
                     /translation="MMPTINFNGKTYNSIEEMPANERQAFEKLSSLLVDANGNGIPDL
                     LEGDMVQNVMAVHATKTSINVNGQTYHSLEDLPPDLRQSVDGAFQFLSKMGIANTTSD
                     IQTPAMRQKPQQKSKPFLAQESPSAIEEENGRSTFSIVLISIVLCFAVIAATIAIFVF
                     INR"
     gene            8927..9331
                     /locus_tag="PLT08_00645"
     CDS             8927..9331
                     /locus_tag="PLT08_00645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4332 domain-containing protein"
                     /protein_id="HNE02995.1"
                     /translation="MPTLKDIEGIGPVNAGKLNKAGVRGLNGLLKLGASRKGRQEIAK
                     ATGINAKVILEWVNRADLFRVKGIGGQYSDLLEAAGVDTAVELSKRQPEALLEAMKKA
                     NAKLNKVNQMPGLSNVKTWIKNAKSLKRVIEY"
     gene            complement(9379..>9791)
                     /locus_tag="PLT08_00650"
     CDS             complement(9379..>9791)
                     /locus_tag="PLT08_00650"
                     /inference="COORDINATES: protein motif:HMM:NF012382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HNE02996.1"
                     /translation="ADLHTSSFNQQVAYEVGKGGFLDEHVKVIRATYKERRDVMLEMM
                     DEMFPAEMRWTKPQGGMFLWGMMPDGVDAADVLRIAIERKVAFVPGAAFHPNGGGDNT
                     MRINFSYSSPEMIREGITRLGSTLKEVLKKNGQH"
BASE COUNT         2642 a         2087 c         2515 g         2547 t
ORIGIN      
        1 gacaggaaac caactctatt tagcaactac cgcgcctgct ttaccgcccg aaatgccaga
       61 tcaatttcgt ttcgccaatc tggctgacca cccccatatt caaactacac ttgcaaaagg
      121 ctcttctgtc atcatacctg ataccgaagt tgccgaacta accgaagctg agcgtgaaat
      181 ctgcattact agaggtttgc gttcgattgc atacttgcca cttatgagtg ctcgtacagc
      241 aataggggta ttgattgttg cttcaacaaa gaaattacgc acatttaccg atgaagagtt
      301 acttctttat catggctttt cagttcaggc tgcccaaacg attgaaaatg cacagttata
      361 tgattctgct agagttcatt ccaatgaatt ggaacagcag atttctgaac gcaaacaagc
      421 cgagaagaag ctacaggaat cagagaagct ttatcgttat gctatggaag tggctggcac
      481 ggtgccttat tacgagtcat atagcgagaa gcatcccgcc gttacatatg aatttattgg
      541 agaagggatt cgtcagatca ctggctatgg acctgaagaa ttcaattcac aaatttggga
      601 ttcgttagtg gaaaaggcaa taccggtaga ggaacttgaa ggctattcgc ttgatgaagc
      661 cattcaaaga acccgctctg gcgaatttcc gatctggaag tgtgaattcc ggctgcatga
      721 tcgcgatggg aaaacccatt gggtttttga agcggcagtg gaattaagag atgccaatgg
      781 aaaccctgtc gggtccatcg gttcttatca agatatcacc attcgtaaac aagcagagga
      841 aaaactacga aagagtgaag agcagtaccg cttattgttt gaagtagcta gtgatggggt
      901 ggtgctgcat ttgctaagta ctgatcgcat taagaatcgg ttccggcgtt tcaatcaaaa
      961 tatttgccag atgttgggat ataccgctga ggaattggcg caattgagtc caatggatat
     1021 ccaacagcca gaagaacttc tcaaggtgcc cggagaagca gaacagttgg agcggaatcg
     1081 tgaattaagg ttcgagaaag tatttcgccg taaagatggt tccgtatttc catccgaagt
     1141 ccattccagt gtctttgaat tagatggcga gcttctggtg ctttccatca tccgtgatat
     1201 ttctgagcgc aaacaagcgg aggcgaagtt acgtgagagt gaagaaaaat atcgcggctt
     1261 gatggaagcg atcgatagtg tggtggctac gattgattac aacgggagat tcctgtactt
     1321 gaatgaggtt gctgccaaat ggctgggtgg cacaccccaa gaactggtcg gtaagaccat
     1381 gttcgatctt tttcctgagc cgatggcatt gcaacaaatg gaaggcatac gtgaggtctt
     1441 tcaagataat cagagtaaag tgagtgaatc actgacctat attcaggggc agtatcgttg
     1501 gtttcacaac tcactccaac cgatccataa tgaattgggc gaagtagtta atgtgttgct
     1561 tcatgccgtt gatgttcatg aacttaaggt cgtacagcaa gaattggcag atttgaatcg
     1621 atctcttgaa cagcgtatag aggaaagaac gattgaagtg cgccagacaa atttggcatt
     1681 agaacgcgct ttgcttgcca aagatgaatt cctagccaac atgagccacg aacttcgaac
     1741 tccgctcaac ggcattttgg gcatggctga gatcctgctt gaagaagaat atggcttgct
     1801 caatgagcgt cagcatgcat atgtgaactt gatcgattcc agtggacacc acttgcttag
     1861 cctgatcaac gatgttcttg atttgtccaa agtagaagta ggcaaattgg aactggatac
     1921 tgaaaaagtt tttatagaag acctttgtca atctagtatg gtattcgtaa gacaactggc
     1981 aaagaaaaag aatgtgctgg tgaattacct gcccgacccg gcaatttcca cgttacaagc
     2041 cgatgccagg cggctcaaac agattttggt taatttattg agcaacgccg ttaaatttac
     2101 gccagagggt gggaaggtca tgcttgaggt acatgccaat cgggatgaag gtattgtcac
     2161 cttctctgtc accgatacag ggattggcat ctcggaagat aaccaaaagc gtctattcca
     2221 gccgtttacg caagtggatt ccagcttgag ccgaaaatat gatggcacag gcttagggct
     2281 ggcactggtg aaacgattaa ctgagttgca tggcggtacc gttgctcttc aaagtgaagt
     2341 agggcatgga agttgtttca ccgtctcttt gccttggcgt gaggctggtt ctacctcaca
     2401 taaattagat ggtgcggatg ccggagacaa tcataaattt gctccttctg ctcaaacgat
     2461 acacgccact gttttattag tggaagacaa tacctataac gtaatggcga ttagcgagta
     2521 tctcgaagcc aaaggctaca gattggtatt tgccgtagat ggtttggacg ctctagcgaa
     2581 ggcagcggag aattctccgg atttgattct gatggatatt caaatgccca acatggatgg
     2641 tttggaagcc acccgtcgct tgcgatctga ctctgccttt gcttccattc ctatcattgc
     2701 ccttacggct catgcgatgg gaggagaccg ggaacgttgc ctcgcagcgg gtgctaccga
     2761 ttacttgagt aaacccgtaa agctaaagga acttgaatca actattcgta aatggctttc
     2821 gagttagcgt ttgcgtctgc gagattccat gatgccataa aaaataacga tggcaatcaa
     2881 catcccggct gtaaataggg tgtaccagtt aatgatgcct gtcttaaatt ctgcagggtt
     2941 catggtggct gcaaccaaga tcaagctgta agcaatgaag atgccttcaa aactggcgcg
     3001 ccaactcgtc cagcgagggt cgaaggagtt cactaacgaa agggttccga tcaccgaaag
     3061 ccaaccgcat aagacccgtg ccgtgagcgg agtcaactcc cagggccaga tcgggatgat
     3121 caggcttggg atgagataac agatcgtaac gagaagcagg acaagcccac caaagaggcg
     3181 gagaataagt cgcgccgtgg tagggacggt cacatccgat tcttctggct catgcgaatc
     3241 agtattgcgg ttgtatatcc acagagcagg aatcaaaaac ggagagacaa tatataagat
     3301 caaccaggca aggaagggaa tggtgccaag agaaaagcgc tcccaatgcg gaagcgtggc
     3361 agccaacata gctgtggtga agatggttac cgctgggaag acggcttgca cccgatgcca
     3421 acgcggaccg aaagccgttt gagcgagaat ccacgcgccg cccaaataag cggaaccaaa
     3481 gaacatggag gacatgtgag gtttgattgc ccatgcgaac aatgtgccgg tttggtctgg
     3541 caaaaagaat aagataccaa aggcggcaac tagaaacaaa acaacgattc cggcaataat
     3601 acgagtaagg gggaagataa catctgattt catcttggtt cctgtattga taaagtttac
     3661 ctagggcaac gcccctgcaa gtggaatgac ccagcgctct tcaaaggtgt tgatcatggc
     3721 aatgtttggg tcttgtgggt catagatcac atccaacgat tcgccttgcc aactatggtc
     3781 gagggttggg tagtaggcat tgcctgtgac ggtgtagggc tgtccatcta cttcgaactc
     3841 tgctgttaag atgaaaacgg cgttatgacc cgcgctgctt ttgaattctt ctaaatcgat
     3901 aaccgttcca gtggttgtgc tgcccttgaa ttgcaggtct atggaggttt gtagcatttt
     3961 gatcttgtcg acgttgaaga gaaagaaaaa actgaccaca caagaaaaga ccaaagcaag
     4021 aataataaag ctggcttggt tgccgatgcg tttggcggtt ttctcgttgg ggatatctga
     4081 tggttgcatg ttgttctcct tagtaaagaa acacgaacaa gaccgttccg tcacgaacaa
     4141 acaaaatgtt tgcaatggga gtttgggggc atttgtgata aatccaaaag tcatcctccc
     4201 tctgtgggat gtttgtcttt ttcactcttc atataatacg agacgaatac atttcatgga
     4261 gcaataatga tgaagagaaa tcaacctatt cttatgttcg tggctgtttt aaccattgcc
     4321 gccttagctt gtaacctgcc cactacctcc aacccagccg atgccaccga agagccgacc
     4381 gaggtaagta ccgaagcgcc tgaagccaca cagacagaag cacccactga agaggcttca
     4441 gatagcgtgt ctgtctctgt aagcaccgcc accaattgcc gtacgggtcc agatgttgtc
     4501 tatcagttat tgatgactgt tcaaccgggc agcgattttg atgttgttgg gaaatatact
     4561 cctaaaggat attggatcat caacatgcct acgggtggaa cctgttggct gtggggtcaa
     4621 tatgctattg tgcaaggcga taccagtgca ctacccgaga ttacaccgcc gcccgctcct
     4681 gttgcgcaac ccaccagtga aacttcaaat caagcgaata attcaaatac aaataataat
     4741 gaggaagaag acccgcaacc ttccgcgcct gcgcctgctg ctcccaccaa tacagatatt
     4801 acccgtcagt gcaccaccct tacccaaccc ggtgacctct tcccaagctt caagatggta
     4861 gtgcagttca catggaatga tgcttctgat aacgaaacgg gctttaacat ctacaaagac
     4921 ggaacttttt atacaacttt gcctgccaat actcaaaaat tcacagagac attcacagtc
     4981 ttattggcac agtctccctc tataacttat ggagtgcaat ctgtgaatgc aaatggcagt
     5041 tcaggtcttg tgacaggtac agtgagttat tgtaagtagt tgaccggttt tataaaacac
     5101 cctgtttctt tgacagggtg ttcttaaatc aataaaggag ataaaaatgg ctgaaatgac
     5161 ctgcccaaaa tgttctggcg caatggaaga agggttacag tttaatttta agttgaatga
     5221 gctatcagat gactatgtta tcgaaacggc tgaactgacg cgtgatcgct ggcaaaagac
     5281 agaagatgcc acaggtaaat tctttgggaa agaatattta ggaaagcgcc gggtgggtcc
     5341 gcgtttggcg gttattactt atcgatgcac agtatgcgga tatttagaat cctatgctcc
     5401 gcctgtaaaa taaaaaacta cctgatcgaa ctttagttgg gctgtttttt tatgtaattt
     5461 acatcattcc ataaaccagc atatacacgt cgttgatggc ttctactttg agagcatcta
     5521 gcccattggc gttgagttgc tcgcggactt cttcaggggt gaacgcggca cataacgaag
     5581 cggcgaaatc tttttgtaga atgggatgct cgttcccggc tcgttctcgg acgagttgct
     5641 cggcggcttc cacactttcg gtgcgctgca aatcggagat gaagaccaat gtccctgggc
     5701 gggtgtgagc gcggatggtt ttccataaag cattcgggtc gtggaagtga tgcaatgcac
     5761 tatgcgacat tacaaggtca tacttgttct gggggatttc atcgctgggt aaaaaagctc
     5821 tcacaaattg aatccggttt gcgatttcag gctcggcttg cacatctttt tgagcgatat
     5881 cgagcatggc ttgcgaaccg tccacggcga tgatgcggct gtgtgaaaag gctcgggcga
     5941 agcgaaaggt catatcgccg ccaccacaac acaggtccag aatggaggcg ggttggttgt
     6001 ccttcccaaa acgttcctgg aaccaagtca cacgcatttg gtgtgaagtg gcaaaatctg
     6061 catgtgaata ggcgagtgct tgttccatat cttccatcag ctcgggttca agaattcttt
     6121 ccatagtgac ctctattaat aaatttattt actgcaaccc acagatgctt tttcaatggg
     6181 caaaccgaag gcttcataaa ctggcacacc atcccattcg gtgggaatgt taagcccgag
     6241 ggcaaaggca gcggtagcgg cagtatcaac tgtggtgata gacgaattga gcgcggctgg
     6301 tttaatgcct gcaccaaatg cgatccatgg aatggtcata tcttcgtaga gactggtacc
     6361 gtgcgttgtg gcatgcccgc catgatcggc agtgatgatg aatagggttt cagacatcaa
     6421 accgcgattg ttgagttcgg tcacgagttt ttcaagcgcc tgatcggcac gaaacaacac
     6481 gctaagctgt tcgccagaaa gccagccata ttcatgcccc atccagtcgg tggtagggaa
     6541 gtgaacgaac atcaaacgaa aatcggtggg gaagttttcg atcagattgt cggtgatgac
     6601 taggtcgcgg tcgttgataa atttgaagat gtctgtgctt tgcggttcgg tgatctgacg
     6661 taatttttct ttgcccacat acataacagt ttgcatacca gccgcgtgga cgatatcgaa
     6721 aaggtcggtc acttgagcgt agccattttc aggaatgtag tcgttccaat ccactttgtg
     6781 tttggcgggg cattgtccgc taagcatgga ggtatgtgag acaagagtca cactggggta
     6841 tacagtttgc gcagtcagtg aatatgctga ttgactcaac agtgatgtga gaaaaggcat
     6901 cggggcgagc ggaatggcat cggggcggaa cccgtcgatg gagagaatca agacgcggcg
     6961 agcgagaggc gtaggggtgg gcgtaatcga gggtgttagt gttggctctg gtgtgagagt
     7021 ttgagtcggg gttgatgtaa ccgttgcggt aggagtttgc gttggcgttt gagtggcaag
     7081 gatgggaatg gacgggagtt ggcaggcaag cgccgtaaga caaagaataa caataagtcg
     7141 gatgcgtgac atttgtccat tataaataac tttcagcgta gtatgatttg aatatggata
     7201 ctgaaacctc tcgaactttg catgaagaat tgattcatca taacaacgtg gacccacata
     7261 aacgtggctc gggtctttcg gactttattt tgggtgcgca agacggcttg gtcaacgtcc
     7321 tcggcgtggt gctgggaatc gctgccgcga cgaacgatgc acgcgtggtg ttggtcgcag
     7381 gcttggcaac cacctttgcg gaatccattt cgatgggggc agtggctttc accaccactc
     7441 tcgctgatgc ggacctatat caaagcgagc gtgaacgcga atatcgacat attgccgaag
     7501 cgcccatgct tgagacaaaa gagatccgcg atatttatgc cagcaaaggc tttacagggg
     7561 acttgttaga ccgcatcgtg gaaacaatca ctgcgaacaa agatgtgtgg gtggcggtga
     7621 tgatggcaga agaacatcaa ctctcccctg tagatcgtgg cactgccatg aaggctgctt
     7681 gggtggttgg gctttcggca atcattggtt cgttggtgcc gatcttgccg ttcatcttct
     7741 taccagtttc gatcagtatg tggctctcgg tattggtgac ggcgttggtc ttgtttggaa
     7801 tcggctttta taaagcgcgt gtgaccgtag gcaagcccat gcgcagcggc acagagatga
     7861 tgctaattgg gacgatcagt gctatggctg gatatctcgt cggggttgtt ctcaaggttc
     7921 cgccccttcc gtgatggttt gctgaaatat aaatttgcca gcatgccaag gaagaagaca
     7981 aagagcatcg ctacgatcac ttctgcaccc cagccaatga gcggaacgaa agtgagaatt
     8041 tcaagcgcgg cgtcttttaa cacggtggaa agtccataca gtgccatggc tgaatagcca
     8101 atttccttga accctaattt ggattcattg ctttttgaga ggtgtacgat catgtacacc
     8161 tctaatgctg ttaatagcag ggatgtgcca gggattggcg cagtgatggc ggagatcacg
     8221 ccggcgataa gggtgtaaat gaggacgagt ttcatcggtt gataaagacg aaaatagcaa
     8281 tcgtggcggc gataacagca aagcaaagca caatgctgat caaaacgatt gagaaggtgc
     8341 tgcgtccgtt ttcttcttca atcgcggagg gagattcttg cgccagaaat ggcttggact
     8401 tttgttgcgg tttctgacgc attgccggag tttgaatgtc tgacgtcgtg tttgcgatac
     8461 ccattttcga taagaattga aaagcgccat ctacactttg acgcaaatct ggtggcaagt
     8521 cttcaagaga gtgataggtt tgtccgttga cattgatgct cgtttttgtg gcgtgaactg
     8581 ccatcacatt ctgcaccata tcgccttcaa gcaggtcggg aatgccgttg ccgttcgcat
     8641 ccacaagtaa gctggataat ttttcaaaag cctgtctttc attggcgggc atttcttcta
     8701 tgctattgta ggtctttcca ttgaaattaa tagtgggcat catgaactcc tgcgtcttgt
     8761 ctctccattt tacacgcaga tattaccaaa cctaaaagta aagtttgtat cgtcagtttt
     8821 tagataatgt ggaaagaaca ataaaatctt ttgtgcttaa gaatggataa agaattggtt
     8881 ttttagtaat tagatgttat agtattgaat aaggagataa caacatatgc ctacactaaa
     8941 agatattgaa ggaattggtc ccgtcaatgc tggcaaactt aacaaggctg gtgttcgggg
     9001 cttgaacggg cttttgaaat tgggagcttc tcgcaaggga cgccaggaaa ttgcaaaagc
     9061 tactggtatt aatgcgaaag ttatccttga atgggttaac cgtgcagacc tgtttcgtgt
     9121 aaagggaatt ggtggtcaat attccgattt gctcgaagct gctggtgtgg atactgcggt
     9181 tgaactttcc aaacgccagc cagaagcctt gcttgaggca atgaagaagg caaatgctaa
     9241 actgaacaag gttaatcaaa tgcccggctt gagcaatgtc aaaacttgga tcaagaacgc
     9301 gaaatcactc aaacgtgtga ttgagtacta aataaaaaaa tcccgatgtg atgaacaatc
     9361 gggatttttt tatttaactt agtgttgtcc gttcttcttg agaacttctt tcaacgtaga
     9421 gcccagcctt gtaatccctt cgcggatcat ctctggtgac gaataagaga agttgattcg
     9481 catggtgttg tctccaccgc cgttagggtg aaaggcagcg cccggcacaa atgccacttt
     9541 gcgttcaatg gcaattcgca atacgtctgc ggcgtccaca ccatcgggca tcatgcccca
     9601 taagaacatg ccgccttgag gttttgtcca gcgcatttcg gctgggaaca tttcatccat
     9661 catttcaagc atcacatcgc ggcgctcttt gtacgtggcg cgaatcacct ttacatgttc
     9721 atccaagaat ccgcctttgc ctacttcata tgccacttgt tgattaaaac tggatgtatg
     9781 taagtctgcg g
//
LOCUS       DAOQJE010000014         9692 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_3713_length_9692_cov_5.458856, whole genome shotgun sequence.
ACCESSION   DAOQJE010000014 DAOQJE010000000
VERSION     DAOQJE010000014.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 9692)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9692)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9692
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3713_length_9692_cov_5.458856"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..792)
                     /locus_tag="PLT08_00655"
     CDS             complement(<1..792)
                     /locus_tag="PLT08_00655"
                     /inference="COORDINATES: protein motif:HMM:NF024367.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3854 domain-containing protein"
                     /protein_id="HNE02997.1"
                     /translation="MTNTLSQPHHDSLRASTITDEVIAARGYRTVTEADELRKLGFAP
                     NQCRVPGLLLPLHTTDGQVGLNVYRPDNPRVVEDRRKTNPDGTHPVRVMKYELPKNAG
                     VRLDCPPNCHAQLANPAIPLWITEGQKKADALVSAGMCAIALLGVWNFVGKNEYGGIT
                     FLADWQYIALNDREVHIIFDSDLMTKHPVRQALERITQHLQRKGAHVSAVYLPQQVDG
                     SKAGVDDWLAAGHRVEELGNLLDTPRPAPKPADPLVEILDSTPSTI"
     gene            complement(789..1022)
                     /locus_tag="PLT08_00660"
     CDS             complement(789..1022)
                     /locus_tag="PLT08_00660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE02998.1"
                     /translation="MQLHDHPELEAQKRQALAKVYALLIRLTDDREKQTAHPDNFDET
                     TGKAENITPTCVKVNVDKLYTHTQGGYKGVYKK"
     gene            complement(1009..1206)
                     /locus_tag="PLT08_00665"
     CDS             complement(1009..1206)
                     /locus_tag="PLT08_00665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE02999.1"
                     /translation="MEGIKIYVLIEDKENEAEIRIDGNLETLRGLIYILYTEIANSAN
                     DNTVPTQSNKVKQEDLVHAVA"
     gene            complement(1301..2014)
                     /locus_tag="PLT08_00670"
     CDS             complement(1301..2014)
                     /locus_tag="PLT08_00670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE03000.1"
                     /translation="MKTGQLHSVTYLATYNIDFMERMLRRVPGHSGGSFVDHCNFQKV
                     QPASSHQGWDVRYNVYKESSDYPPSGIYKDDDVIIFEDAYIGNITLLKHENPEDRTSI
                     TRVTLECFEEFDDTFRKIKREWDLFIKSDMEITRLVLSGREAAPSNSSATGQNTEWIV
                     ATNKPKRPNGQIHTRAMIWRDLKGWMKIGSVSPSPQECITELKKKPAYKNKKISTKRM
                     QKILSEGFAGMYDDILKNI"
     gene            2518..2661
                     /locus_tag="PLT08_00675"
     CDS             2518..2661
                     /locus_tag="PLT08_00675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE03001.1"
                     /translation="MPEKEKPQPPPPPKKDDSATKSTGLAGDSSTNAPPPPPKKEEEK
                     KEK"
     gene            2663..3142
                     /locus_tag="PLT08_00680"
     CDS             2663..3142
                     /locus_tag="PLT08_00680"
                     /inference="COORDINATES: protein motif:HMM:NF025056.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003953 - NAD+ nucleosidase activity
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="toll/interleukin-1 receptor domain-containing
                     protein"
                     /protein_id="HNE03002.1"
                     /translation="MAERKLRVFLCHAQEDKKVAQDLYKQLAAEPWIDPWIDKEHLLP
                     GQDWKHEIEKAQSEADVIVVALSSHSVTKDGFVRKELSRVLEQAAEKPEDEIFIIPLR
                     LDESPVPPSLANHRRIDLANDPKPLINALKVRANFLLPGTSTTDAPPPATKKKSGRK"
     gene            3280..4362
                     /locus_tag="PLT08_00685"
     CDS             3280..4362
                     /locus_tag="PLT08_00685"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR01251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0002189 - ribose phosphate
                     diphosphokinase complex [Evidence IEA];
                     GO_function: GO:0004749 - ribose phosphate diphosphokinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate pyrophosphokinase"
                     /protein_id="HNE03003.1"
                     /translation="MTRRSIPADEVRFFSGSSNLQLAERIATEVGAPLDAAQIGRFSN
                     DNLYIQLGASMRYRRVYIVQSLSTPVNDHLMELLMMLNIARSSAASEVTAIIPYYSFA
                     RSDKKNAPRVSIDARLVADLLKTAGATHVISMTLHSPQVEGFFSIPIDALSSRAVFNK
                     YLSDSDLSNSIIIASNLDQTRPAVRLANALNVPVAASTMERDSDNRTTTTGLIGNQLR
                     GYKRALIYADEIATSGSTLALSEMLIKEGVEEIRILCTHGVFVRGAIEKLAAIPQITE
                     VITTNTVYIPSEKRHPKLTVLSVAGVFADAIRYNFNQESIADLFVFGGESVEALHGSS
                     TTEAPPPLMEKIKTILKNKKQKAKKK"
     gene            4452..4835
                     /locus_tag="PLT08_00690"
     CDS             4452..4835
                     /locus_tag="PLT08_00690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE03004.1"
                     /translation="MEESKPISLNIEISASNATAEDNNQAARQLQSELKNIGVNSAVM
                     LSSLVIHVLLPAILPAVVETIQSWVSRSSGRSIKFTGKVGGQTIEFEGSPADFKKLIT
                     TLEARGLAGDSTTDAPPPPKKKKDK"
     gene            4840..5034
                     /locus_tag="PLT08_00695"
     CDS             4840..5034
                     /locus_tag="PLT08_00695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE03005.1"
                     /translation="MTELIWQGKYDENCKRTAPLRVSLPFQTVETINESSMERQQMLD
                     LFSAGRDSDWRNRLIWGDKC"
     gene            5134..6975
                     /locus_tag="PLT08_00700"
     CDS             5134..6975
                     /locus_tag="PLT08_00700"
                     /inference="COORDINATES: protein motif:HMM:NF012862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="caspase family protein"
                     /protein_id="HNE03006.1"
                     /translation="MQGQPNNIYVGDPLSILQEFENDQFDLVYFDVPYFESNSFRKND
                     IQIPYQSITEYIEYLARIIIHLRHALKDNGSLLLRVNPLSPFNARLFLDRVFGKQNLR
                     AEIIWERKATGLRSTSTPYANFESVFFYSKTDRFVYHLPTRALTHDEIKRVYHYEDEK
                     GLYGLLPLIDNLSRPKGSFEWKGFTPPKGKSWRYSEDRLNALYQEGNIEFSKKTPQRK
                     NYLEGAREIPLGFVWTDTPVFNLKGGPYDPRLQRAIELASDSSANILCLFSDLASLNT
                     IQKSNLESAANRQWVSITPFDPAINLLKEFQKEESRWINYLRDSGINVQVNILDMQED
                     RKRELPSHIQKLVLAESQSLDASTLSATTGQKYAFLVGINQYESGIRELNYCVNDAIK
                     LGATLQKHGYQVRILHTEVEDSNLFPRRSSIIDELAAWTQNLLPEDTLYVHFSCHGDL
                     IGEESVLIVSDTRHRSISKTALKLNDVIETMKSGRARKLVLSLDVCHGGVDMGRALID
                     KAFMQNVYENAEGFVVLAASTAHQKAYEVHEYQHGLFTYFLIKGLSGDADADENGVIS
                     VEDIRNYVLHQISQWNMQNHKRQEPTYRAEGIGEIILVNSSMTHSNL"
     gene            7006..7455
                     /locus_tag="PLT08_00705"
     CDS             7006..7455
                     /locus_tag="PLT08_00705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE03007.1"
                     /translation="MANEIIKIKIGESLELIIEGLPASIITPFVQEKVAILLVKQGLP
                     NAGVISAGILGGVRTATLQIGGENYQLKYSINVLKTVTALKVYEEAVAWIKFQETANR
                     VDADLANDAIDYLRKEFSHSFGANERGGLYGGSTTDAPPPSKKKKDK"
     gene            7460..9439
                     /locus_tag="PLT08_00710"
     CDS             7460..9439
                     /locus_tag="PLT08_00710"
                     /inference="COORDINATES: protein motif:HMM:NF013704.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006306 - DNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methyltransferase"
                     /protein_id="HNE03008.1"
                     /translation="MTELIWQGKYDENGKRTAPLRVSLPFQTVETINESSMERQRMLD
                     LFSAGRDSDWRNRLIWGDKRYVLPSLLDEFAGKVNLIYIDPPFNVGADFSFTATVADD
                     PESDDDETTSFVKEPSLIEQKAYRDTWGRGLDSYLQWFYETIVFLRELLSENGSIYVH
                     LDYNIVHYAKVILDEAFGENNFHGEIVWKRADSHNDAHTYGAVHDTILFYSKNGEPLF
                     NPEYVPLSQKTVDTWYRHVEPETGRRYNLGNLVSPHPRPNLTYEYNGIKPPPFGWRVS
                     RQRMEEYDKKGLLVVTGKKQPTLKIKQYLDESRGRQVTDWWDDISQLRGYATSQEERT
                     GYETQKPEALLKRIIKASSNENDLVLDCFCGSGTTAATAEKLNRRWITCDLGRFAIHT
                     ARKRLLAIEGVKPFMVQNLGKYERQAWMETQFTQQEAQQRYREFILNLYHAKPLNSYT
                     WLHGIRGGRMIHVGSVDAPVTAGDLTSIAAEFKKSVGSGKDAPKSNGVDILGWDFAFE
                     LNEVAKQQAAQANLDVRFFRVPRDVMDKRAVEQGDIKFFELAALKVTTKVSKKKELSL
                     TITDFVIPPDDVPQDVQKAIKHWSQWVDYWAVDWDFKGDTFHNQWQTYRTRKNPDLQK
                     SATHVYESAGEYTVVVKVIDILGNDTTKTIKVQVG"
     gene            9442..>9692
                     /locus_tag="PLT08_00715"
     CDS             9442..>9692
                     /locus_tag="PLT08_00715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE03009.1"
                     /translation="MPRKKQTTSAAQTSFVEPIAKTAPCVPAIRRAVEEWRVKEYPGV
                     SETTRILLNFWFKTDHRLYNGRKFAYHKSQREAIETLIYL"
BASE COUNT         2722 a         2217 c         2262 g         2491 t
ORIGIN      
        1 gatggttgat ggggtagagt ctagaatttc caccagggga tctgccggct tgggcgccgg
       61 gcgcggggtg tcaagcaggt ttcccaattc ctctactcta tgaccagcag ccaaccaatc
      121 atccacaccc gctttgctac catcgacttg ctggggaaga tacaccgccg atacatgtgc
      181 gcctttgcgt tgaaggtgtt gggttattct ttccagcgct tgccgcactg gatgctttgt
      241 catcagatcg ctatcaaaaa taatgtgtac ctccctgtcg ttcaaagcga tatattgcca
      301 gtccgcgaga aaggtgatgc cgccgtattc attcttgccg acaaagttcc acacgcctaa
      361 tagggcaatg gcacacatgc ctgctgaaac aagcgcgtcg gcttttttct gtccttcggt
      421 gatccatagc gggatggcgg gatttgccaa ttgtgcatgg cagttgggtg gacagtccag
      481 ccggacgccc gcattctttg gtaattcgta cttcatcacc ctgactggat gtgtgccatc
      541 aggattggtc ttgcgccgat cttccaccac acgcggattg tccgggcggt agacattcag
      601 cccgacctgc ccgtctgtgg tgtgcagtgg cagtaacaga cctggaacac ggcattgatt
      661 gggtgcaaag cccagtttac gaagttcatc cgcctcggtg acggttcgat acccgcgcgc
      721 cgcaatgacc tcatctgtga tcgtcgaagc gcgcaggctg tcatgatgag gttgcgaaag
      781 ggtattagtc attttttata cactcctttg tatccccctt gcgtatgggt gtataattta
      841 tccacattca cttttacgca agtaggggta atgttctccg cctttcctgt cgtctcgtca
      901 aagttgtcag gatgggcggt ttgtttttct ctgtcatctg tcaggcggat caacagggca
      961 tatacctttg ccagcgcctg gcgcttctgt gcctctaact cgggatgatc atgcaactgc
     1021 atgtaccaaa tcctcctgct tgaccttatt ggattgtgtc ggcacggtgt tgtcattggc
     1081 agaattggcg atctctgtat ataggatgta gatgagtccg cgcaaggttt caaggttgcc
     1141 gtcaattctt atttcggctt cattttcttt atcttcgatc aaaacatata tttttatgcc
     1201 ttccatttat cacctcgtat aaaacaaacg actcttatgc taaaagtata agagtcgttt
     1261 gacggtggtt cttgaaaatt tacgacgatt tgagatgttt ttagatgttt ttcaatatgt
     1321 catcgtacat tcctgcaaaa ccttcagaaa gaattttttg catgcgtttt gtactgatct
     1381 tcttgttttt ataagcaggc tttttcttga gttctgtaat acattcttgg ggtgaagggg
     1441 atacacttcc tatcttcatc catcctttta gatctctcca tatcatggcg cgtgtatgaa
     1501 tttgcccgtt cggcctttta ggtttattgg tggcaacgat ccactccgta ttctggccag
     1561 tcgcagacga gttcgatggt gccgcttctc taccggataa aaccagtctg gtgatttcca
     1621 tatcggactt gataaataaa tcccattccc gtttgatttt tctgaaggtg tcgtcgaatt
     1681 cttcgaaaca ctccagagtt acccgtgtta tggatgtcct gtcttccggg ttttcgtgtt
     1741 tcagtaaggt aatgttgcct atatatgcat cttcaaaaat gatcacatca tcatccttgt
     1801 agattccgct tggcgggtaa tcgctggatt ccttgtatac gttatagcgc acatcccagc
     1861 cttgatgaga tgaagcgggt tgaacctttt ggaagttgca atgatcgaca aaactcccgc
     1921 cggaatgccc cggcactctt ctcaacattc tttccatgaa atctatattg taggttgcga
     1981 gataggttac cgagtgaagt tgacctgtct tcatctcttt acatcctttc gctttgacac
     2041 gcctggatgt gttatagtgc cctttagggt acaacgccca catcacaagc cgccgtaatc
     2101 ggctttgatt ttgccctggg tattgcagta ccgcagggcc ttttgggcat tttagcacaa
     2161 gactcagatt acaaacatca gagttgcagg caatggggca gcggttcaag gtaggataaa
     2221 aaaccagggc cttattcagt tagttgtatg gtttgggaag gaaaagcaac caagaaaccg
     2281 agtggacatc gccaggcgga tgcatgctgg cagagacatt tttcagtcga cagaattccc
     2341 ctttggttct aaaatcaagg caaagccccg tgggagtggc atctacgagg ctttgaaaaa
     2401 gactacgcga ttcgggcgcg tgtcgttagg gtgatgatag ccataaatcc taccgcctgc
     2461 gcagattgcg taggcggttg tcgttaaagg atcaggacat tttttaggag cgcaaccatg
     2521 ccagaaaaag aaaaaccgca acccccacct ccaccgaaga aagacgactc ggcgacaaaa
     2581 tctaccggcc tcgctggtga tagttctaca aatgctcctc cgcctcctcc caaaaaggaa
     2641 gaagagaaaa aggagaaatg atatggctga acgtaagctt cgagtttttc tatgtcatgc
     2701 gcaggaagac aaaaaagttg cgcaagattt atacaaacaa ttagccgcag aaccttggat
     2761 tgatccttgg atagataaag aacatctgct tccaggtcaa gactggaaac atgaaattga
     2821 aaaagcccag agtgaagcag atgtaatcgt tgtggcattg tccagccatt cggtgacgaa
     2881 agacggattt gttcgtaaag aattatccag agttcttgaa caggctgcgg aaaagcccga
     2941 agatgaaata ttcattattc cgcttcgact cgatgaaagt ccagtgccgc cttctttggc
     3001 aaatcatcgt cgtatagatt tggctaatga tccaaaacca cttatcaatg cgcttaaagt
     3061 aagggcaaat tttcttttac ctggaacttc tactactgat gcccctccac cagcgacaaa
     3121 gaaaaagtca ggacgcaagt aaattaaatc attatcagga tgccctctta tgaccgaact
     3181 catctggcaa ggaaaatatg ccgaaaatgg cgcgaccgtt aagattgagt tggatgggaa
     3241 cgttgttgaa tatcccctgt tccaaaagat ggaggcgaga tgacaagaag atcaatcccg
     3301 gcagatgaag tccgtttctt cagcggcagc tcaaacctgc aattggcgga aaggattgcg
     3361 acggaagtag gcgctccgtt ggacgctgct caaatcgggc gattcagcaa tgacaacctg
     3421 tatattcagt tgggcgcaag catgcgttat cgccgcgtgt atattgtgca gtctctttcc
     3481 accccagtta acgatcactt aatggaattg ctgatgatgt taaatattgc gcgcagctca
     3541 gccgcatctg aggtgactgc gattattccc tactattcct ttgcacgctc agataaaaag
     3601 aatgcccccc gtgtttcgat tgatgcccgc ctggtcgctg atttactaaa gactgccggt
     3661 gctacgcatg tgataagcat gacgctccat tccccgcaag tggaaggttt ttttagcatt
     3721 cccatcgatg ctcttagtag tcgcgcggtc ttcaataaat acctttcaga ctctgacttg
     3781 agcaactcta ttattatcgc atcaaatttg gaccaaacca gacctgcagt acgcttagca
     3841 aatgccctta atgtaccagt tgccgccagt accatggaac gtgattcgga taacaggaca
     3901 actacgacag ggctgattgg aaatcagttg cgcggataca agcgtgcttt gatatatgcg
     3961 gacgaaatag cgactagtgg ttcaactctt gcgttgagcg aaatgctgat caaggaaggt
     4021 gttgaagaga ttcgtatttt atgtacgcat ggtgtgtttg tacggggtgc aattgaaaaa
     4081 ctggctgcca tcccgcaaat caccgaagtc atcacgacta acacggttta tataccttcc
     4141 gaaaaaaggc accccaaatt gacagtattg tctgtcgcag gtgttttcgc ggatgcgata
     4201 cgttataact ttaaccagga gtcaattgcc gacctgtttg tgtttggcgg agaaagtgtg
     4261 gaggcattgc acggcagctc gaccaccgaa gcgccccctc cgcttatgga aaaaataaag
     4321 acaatcctaa aaaacaagaa gcaaaaagcg aagaagaaat aaggtatgca agggtcttaa
     4381 atttggattt accaggaacc cccttatgac cgaacttatc tggccaggaa agccaagcaa
     4441 aggattgacg catggaagag tcgaaaccca tatcattaaa tattgagatt tcagcaagca
     4501 acgcgacggc tgaagataat aatcaggcgg cgcgccaatt gcaatcagaa ttgaagaaca
     4561 taggcgttaa ctcggctgta atgcttagtt ctcttgtaat tcacgttcta ttgccagcca
     4621 ttctccctgc agttgtggaa actattcaat cctgggtatc acgctcaagc ggtcggagta
     4681 tcaaattcac aggtaaggtc ggcgggcaaa cgatagaatt cgaaggctct ccagcagatt
     4741 tcaaaaaact tattacaaca ctcgaggcac gcggacttgc aggcgactcc actaccgacg
     4801 ccccgcctcc acccaaaaag aagaaggaca aataacctta tgaccgaact catctggcaa
     4861 ggaaaatatg acgaaaactg caagcggacc gcgcctttgc gcgtctcgct cccattccaa
     4921 acggtggaga cgatcaacga atccagcatg gagcgccagc agatgttgga cttgttcagc
     4981 gcggggcgtg actcggactg gcgcaaccgc ctgatctggg gagataagtg ttaagtgctg
     5041 cccttgctgc tgggcgagtt tgcagataag gcaaacctga ttcacactgc ccatccttca
     5101 attttgtaca cgctttttta aaacaaggac tttatgcaag gacaacctaa caacatatat
     5161 gtgggagacc cacttagtat cttgcaggaa tttgaaaatg atcaatttga cttggtctat
     5221 ttcgatgtgc catattttga aagtaactca tttaggaaaa atgatattca aattccatat
     5281 caaagtatta cggaatatat agagtatctt gcacgaatca ttattcactt acgtcatgcg
     5341 ctgaaagaca atggttcttt gcttcttcgg gtcaatccct tgtcgccatt taatgcaaga
     5401 ttatttcttg atagagtctt cggcaaacag aatcttcgcg ctgaaatcat ttgggagaga
     5461 aaagcaacag gcttacggag tacatccacc ccttacgcta attttgaaag tgtgtttttt
     5521 tatagcaaga ctgatcgctt tgtatatcat ctgcctacca gagcgcttac acatgatgaa
     5581 attaaacgag tgtatcacta tgaagacgag aaagggcttt acggactact tccattaatt
     5641 gataacctat ctcgacctaa aggatctttt gaatggaaag gatttacgcc cccaaaaggg
     5701 aaatcatggc gctattctga ggatcgctta aacgcgctct atcaagaagg gaatatcgag
     5761 ttctcaaaaa aaacgcctca aagaaaaaat tatcttgaag gagcgagaga aattccgcta
     5821 ggttttgtat ggactgacac gccggtgttt aacttgaaag gcggtccata cgatccgaga
     5881 cttcaaaggg cgattgaatt ggcatcggac tccagcgcca atatattatg cttgttttct
     5941 gatcttgcct ctttaaacac aattcaaaag tcaaacttag aatctgctgc caatcgccag
     6001 tgggtttcga ttactccatt tgatcctgca ataaatttac taaaagaatt tcaaaaggaa
     6061 gaatctcgtt ggataaacta tctgcgtgat tcagggatta atgttcaagt aaatattttg
     6121 gatatgcaag aagatagaaa aagagaactt ccgagccata ttcaaaaact ggtacttgca
     6181 gaatctcaat cgcttgatgc ttctacacta tctgcaacta cagggcaaaa gtatgctttt
     6241 ttagtgggca ttaaccaata cgaatctgga attagagaac tgaattactg tgtcaatgac
     6301 gcaataaaac taggcgcaac tcttcagaag catggatatc aagtaaggat acttcacaca
     6361 gaagtggagg acagcaacct gttcccccgc aggtcaagca taatagacga acttgccgct
     6421 tggacacaaa accttcttcc agaggacacc ttatatgttc atttttcgtg tcatggtgat
     6481 ttaattggag aagagtctgt attaatagta agcgatactc gccaccgaag catatccaag
     6541 acagcactga aattgaacga tgttattgaa actatgaaaa gtggacgtgc tcgaaagttg
     6601 gtgctgtctt tagacgtttg ccatggcgga gttgatatgg gcagagcgct tatcgataaa
     6661 gcgtttatgc aaaatgtata tgaaaacgcc gaaggttttg tcgtgcttgc agcaagcaca
     6721 gcacaccaga aagcatacga agttcatgaa taccagcatg gtttattcac ttattttcta
     6781 atcaaaggac tttctggaga tgccgacgcg gatgaaaatg gagtaatttc tgtagaagac
     6841 ataagaaact acgtgcttca ccaaatcagt cagtggaata tgcaaaatca caagcgtcaa
     6901 gagccgacat atcgagcaga aggcattggc gaaataatcc ttgtcaattc atctatgacc
     6961 catagcaacc tataaatacg aaacctccgt ttaggcgcaa ttaaaatggc aaatgaaata
     7021 ataaaaatta aaattggcga aagccttgaa ctgatcatag aaggtttacc tgcttctatt
     7081 atcacgccat ttgtgcaaga aaaggtggcg attttattgg taaaacaggg gcttcccaat
     7141 gcgggagtaa tttctgcagg aatattaggt ggtgtaagaa cagcaacttt gcaaataggc
     7201 ggagaaaatt atcaactaaa atatagtatt aatgttttaa agaccgtaac tgcgcttaaa
     7261 gtttatgagg aagccgttgc gtggattaag tttcaagaaa cggcaaatag agttgacgcc
     7321 gatctggcta acgacgcaat tgattatctt aggaaagaat tcagccacag ttttggtgcc
     7381 aatgaacgcg gcggactata cggtggctcc accaccgacg ccccaccgcc ctccaaaaag
     7441 aagaaggaca aataacctta tgaccgaact catctggcaa ggaaaatacg acgaaaacgg
     7501 caagcggacc gcgcctttgc gcgtctcgct ccccttccaa acggtggaga cgatcaacga
     7561 atccagcatg gagcgccagc ggatgttgga cttgttcagc gcagggcgtg actccgattg
     7621 gcgcaaccgc ttaatttggg gtgacaagcg ttacgtcttg ccctcgctgc tggacgagtt
     7681 tgcgggcaag gtgaacctga tctatattga cccgcctttc aacgtcggtg cggacttttc
     7741 gtttactgct accgtggctg atgaccccga atctgatgat gatgaaacta cttcttttgt
     7801 caaagagcca agcctgattg aacaaaaagc atacagagac acttggggaa gaggcttgga
     7861 ttcatatttg cagtggttct atgaaaccat agtgttttta cgagaattac tttcggaaaa
     7921 cggaagcata tatgtacatt tggattacaa catcgtgcat tatgcaaaag taatactaga
     7981 cgaagccttt ggagaaaata attttcacgg tgaaattgtt tggaagcgcg cagatagcca
     8041 caacgacgct catacttatg gagcagttca tgacaccatt cttttttatt caaagaatgg
     8101 cgagcctctt tttaatcctg aatatgtacc attaagccag aaaactgtag atacgtggta
     8161 taggcatgtg gagccagaga ctggtcgccg atataactta ggaaatttgg taagtccaca
     8221 tcccagacct aacttaactt acgagtataa cggaataaaa ccgccacctt ttggttggcg
     8281 agtttcaaga cagcgaatgg aagagtacga caaaaaaggt ctcttagttg ttactggtaa
     8341 aaagcagccc acattaaaaa taaagcaata ccttgatgaa agtcgaggta ggcaagtcac
     8401 tgactggtgg gacgatatta gtcagttacg aggatatgca acaagtcaag aagaaagaac
     8461 tggatatgag acacaaaaac ctgaagcatt actaaagcgt attatcaaag cctcttccaa
     8521 tgaaaatgat ttagtcctcg attgcttctg tggctcaggc acaactgctg caactgccga
     8581 aaaactcaac cgccgctgga tcacctgcga cctcgggcgc tttgccattc acaccgcccg
     8641 caagcgtttg cttgctattg aaggcgtcaa gccgtttatg gtgcaaaacc tcggcaagta
     8701 tgagcggcag gcgtggatgg aaacacagtt cacgcagcaa gaagcacagc aacgctaccg
     8761 tgaattcatc ctcaatctgt atcacgctaa accgctgaat tcgtacacct ggctgcatgg
     8821 cattcgaggc gggcgcatga ttcatgtcgg ttcggtggac gcgcctgtca ccgcgggcga
     8881 ccttaccagc attgccgccg aattcaagaa gtccgtcggc tcgggtaaag acgcgcccaa
     8941 gtccaacggc gtggatatcc tcggctggga ctttgccttt gaactgaacg aagtggcgaa
     9001 acagcaagcc gcacaggcaa atctcgacgt gcgtttcttc cgcgtcccgc gtgacgtgat
     9061 ggacaaacgc gccgtcgagc agggcgacat caagttcttt gaactcgccg cgctcaaagt
     9121 caccaccaag gtcagcaaga agaaggaact atcgctcacc atcaccgatt ttgtcatccc
     9181 gcccgatgac gtgccgcagg atgtgcaaaa agccatcaag cattggtcgc agtgggtgga
     9241 ttactgggca gtggattggg acttcaaagg cgataccttc cacaaccagt ggcagaccta
     9301 ccgcacgcgc aaaaatcccg acctgcaaaa atccgccaca catgtgtacg aatccgcggg
     9361 cgaatatacc gtcgtcgtca aggtgattga catcctcggt aacgacacaa cgaagaccat
     9421 caaggtacaa gtggggtaat catgccacgt aaaaagcaaa ctacgtccgc cgcgcaaacc
     9481 tcttttgtcg agccaattgc caaaacggct ccctgcgtcc ctgccatacg gcgtgcagtg
     9541 gaagaatggc gcgtcaaaga gtatccaggc gtcagcgaaa ccacgcgcat tctgctgaac
     9601 ttctggttca agacggatca ccgcctgtac aacgggcgca agtttgccta tcacaaatca
     9661 cagcgtgaag ccatcgaaac cctcatctat ct
//
LOCUS       DAOQJE010000015         9408 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_3870_length_9408_cov_5.515450, whole genome shotgun sequence.
ACCESSION   DAOQJE010000015 DAOQJE010000000
VERSION     DAOQJE010000015.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 9408)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9408)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9408
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3870_length_9408_cov_5.515450"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1021)
                     /locus_tag="PLT08_00720"
     CDS             complement(<1..1021)
                     /locus_tag="PLT08_00720"
                     /inference="COORDINATES: protein motif:HMM:NF025042.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4147 domain-containing protein"
                     /protein_id="HNE03010.1"
                     /translation="MQILNACIDAVEPGNAIRRFVQREKNLLFIDNKKYDLNEFGNVT
                     VLGLGKATEAMSLALLDVLGSYPSRGLLIPKQAFASPASGFAVLPGGHPVPTEASVRA
                     GNAAFDLASSLTEHDLLICLISGGGSALMTSPQHGLQLEDLQTLTKLLLACGARVDEI
                     NTLRRHLDRAKGGGIAKSANGARIASLILSDVVGNPLEAIASGPTAPDPSTREDALTI
                     LEKYDLLDKVPVVILGVLETAPETPKPNDPLFERVQNVLVGSNSLAAQAGLEMAKSLG
                     FHTHFLGDTWQGEARDVAKELCGHFNLNYERPFCLIAGGETTVTLRGHGKGGRNQELA
                     LAAAIE"
     gene            1108..2085
                     /locus_tag="PLT08_00725"
     CDS             1108..2085
                     /locus_tag="PLT08_00725"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008465 - glycerate dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycerate dehydrogenase"
                     /protein_id="HNE03011.1"
                     /translation="MRPKVFVSRKIRDAGLNLVLEFCDAEVWDEEMPPTRDVLLEKVR
                     GVDGYLSMLTDRVDAELLDTAGPQLKVVSNHAVGFDNVVVADATARGIPVGNTPGILT
                     DATADIAFALLLASARRIVESEKYIRDGKWKTWHPSMLLGMDLAERTLGLVGFGRIGR
                     AVAKRAVGFGMRILYYSPNSEPEFGAIKMNSLDALLQESDFVSVHTPLNESTRHLVNA
                     DFLSKMKPNAVFVNTSRGPVVDQVALYDALKAKKIFAAGLDVTDPEPLPLDSPLLTLD
                     NCVIVPHIGSASERTRDAMARLAAENLIAGLKGERLPHCINPEVYENRR"
     gene            2178..3497
                     /locus_tag="PLT08_00730"
     CDS             2178..3497
                     /locus_tag="PLT08_00730"
                     /inference="COORDINATES: protein motif:HMM:NF012946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding domain-containing protein"
                     /protein_id="HNE03012.1"
                     /translation="MNRTKNARVCIIGAGPSGITAGKNLIQAGVTNFVIYEKNDQVGG
                     NWVFNDKTKHSSVYETTHLISSRRYSEYFDYPMPADYPDYPSHTQMLAYFQGYAKHFG
                     VMPYIQFNTEVHNAEELPDKTWRITLGNSKVEIFDFLLIANGHHWNPRYPKYEGEFTG
                     EFIHSHEYKRAAPFTGKRVLVIGAGNSGCDISVETARVSTFTAMSMRRGQYIVPKFVF
                     GQPPDVVNGRFYWLPAWIRLPFFYLTLKLAVGKYEDYGLPKPKHGILNAHVTNNSEIL
                     YAIRHGKVHVRPDIKRFNGKQVEFVDGRMEEYDSIIAATGFKISLPFFDPNLLSIEEG
                     VPPLYKNVFHPDHPSLFFIGLVQPLGSIWPLSDLHAKLAANYIIGNYHLPKDIRQRIA
                     RHIEYMKKHYVNSPRHSVEVEFHPHIVELKREIPKSAPEWKHGYSDR"
     gene            3478..4146
                     /locus_tag="PLT08_00735"
     CDS             3478..4146
                     /locus_tag="PLT08_00735"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013980.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family zinc-dependent metalloprotease"
                     /protein_id="HNE03013.1"
                     /translation="MDIQIDNLVRAKRRTIALIVERDGSLTVRAPRRATLNDIYGFIQ
                     EKTDWILRSREKLKAIKPIPKKAYVDGERFLFLGQEYELRLVPPQRPALKFDGGFTLS
                     TSAQERGELAFTKWYKTQALTLFTERVNYYANLHGLIPKEVKVNSAKTRWGSCTSAGT
                     LNFTWRLVMAPLEVIDYVVLHELAHLKVKNHSPRFWKLVESLCPDFKHHRKWLRDHGE
                     TMTL"
     gene            4167..4589
                     /locus_tag="PLT08_00740"
     CDS             4167..4589
                     /locus_tag="PLT08_00740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE03014.1"
                     /translation="MLPETLIEVYEHTEPDYKPLVDYQSWRVALMNYTDDLTPENINR
                     MQKHTETDEVFVLMAGHCILFLGEGEETVTKVHAVDMELYKLYNVKRGVWHSHTFSKD
                     ARILIVENRDTVVENSPFVDLSPSQCTEVLELTKQLWK"
     gene            complement(4624..5472)
                     /locus_tag="PLT08_00745"
     CDS             complement(4624..5472)
                     /locus_tag="PLT08_00745"
                     /EC_number="3.1.1.99"
                     /inference="COORDINATES: protein motif:HMM:NF020039.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMP-30/gluconolactonase/LRE family protein"
                     /protein_id="HNE03015.1"
                     /translation="MKAELFFDAKSNLGEGPSWDAKTQTLYWVDIFEKRVYAGNEILA
                     QFDEYVGFVAPTKSGHLIVGKRASFVDLDIATSQQTVLVTLNESATNRMNDGKCDPSG
                     RLIAGTMDLNETDPTGSVYSYDGKSTRVLFRDVTISNGMAWSPDYKTFYYIDTPACEV
                     RAYDYDIKTGEIANRRRAFEVPKSLGWADGMTSDVEGNLWIAMWGGAQVTRWNPNTGQ
                     LLEQVSVPALHSSCPVFGGKNRNELYITSARKGMSEADLNKYPLSGGLFKVDTKYEGM
                     PTFEFG"
     gene            complement(5472..6398)
                     /locus_tag="PLT08_00750"
     CDS             complement(5472..6398)
                     /locus_tag="PLT08_00750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HNE03016.1"
                     /translation="MLSIYKTTENGLEKLDAIANGTWVNVIDPTPDEMEKLVGWGMEM
                     DYINYSLDQDEMARMERDEDYTFILLRIPIHQPESDIPYNTVPLGIMILGNKIITVCR
                     FDSDIFRALTNGKHRYLKTGKRYRLTLYIFLETAVRYLNLLREINRATEAVEDRLQKS
                     TQNREVLELLKYQKSLTYFATALRSNEVMMERVQKTQLFNYYEEDQDLLEDVLTENQQ
                     AIQMTSIATEILSGMMDAFASIISNNLNVVMKALAALTIILNMPTIVASFYGMNVDLP
                     GEGHPLAFLTVIGLSLALTALATYIFYKRNWF"
     gene            complement(6587..7396)
                     /locus_tag="PLT08_00755"
     CDS             complement(6587..7396)
                     /locus_tag="PLT08_00755"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNE03017.1"
                     /translation="MEYNFGLTAQDYAKHRAGFPDEFFDRVFADGIVNPTASLLDLGT
                     GTGTLARGFAARGCSVIGMDISSQLLEQAKDLSLQQGLGIEFRLGKAEETGLPDASFD
                     VVSAGQCWHWFDRPRAAQEVKRLLKPGGRVLIAHFDWLPLPGNVVDRTERLIQIYNPK
                     WYERFGNKNGSYPDWFRDLREARFEDVTSFSFDIHVPYTAEAWRGRIRASAGVGGSLS
                     ADEIVRFDSQLKSLLEGLKQRQVKEEARDDFVLYVSHCVFAVYGHKPKVKS"
     gene            complement(7400..7933)
                     /locus_tag="PLT08_00760"
     CDS             complement(7400..7933)
                     /locus_tag="PLT08_00760"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HNE03018.1"
                     /translation="MSITPWKILSSRHIYPRFRVDTVEVHNGTTFEPIVFEFRSWANV
                     LALTKNNEVVLVRQYRHGVQETLLELPGGVVNDGESPLDGARRELMEETGYSAEKIIE
                     VGQIYPNPAIQENTIFCYLATDVEPNGEQHFDETEEIEVVLMPLDDVIEMVRQGTFKH
                     ALHVAVLFKALMYLKKI"
     gene            7997..8845
                     /locus_tag="PLT08_00765"
     CDS             7997..8845
                     /locus_tag="PLT08_00765"
                     /inference="COORDINATES: protein motif:HMM:NF014414.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNE03019.1"
                     /translation="MPITTDSVRTYYDENTNLFLKFSGHKKAQNIHRSLWMPDTKSLE
                     DALNTSNALIKAEIESVSQTNARIADLGCGVGASLFYIYPRLQNPSPALGLTLSPVQA
                     RLAQESASQLGLQDHIRFAEGDFTSVPLPSDSLDAVYSVEAVCHAVDPEKYFKEAGRL
                     LRKGGKLILVDDYKSARSFNSSEQKWFDAYIDGWYVPGVRTVKQTIDFANKYGLQLKK
                     NDELTPHLRLRNLPDFLASALLFLGEKLPIKHAIVPSMLGSMALQQCLYMKVAEYRMM
                     VFEKIA"
     gene            8877..>9408
                     /locus_tag="PLT08_00770"
     CDS             8877..>9408
                     /locus_tag="PLT08_00770"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015022.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016758 - hexosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0030259 - lipid glycosylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNE03020.1"
                     /translation="MPKTITLLTSGTLGDVLPYIALGKGLIEAGYNVRVAAPRGFAYL
                     FNAEGGGTPPLQFTPFEGNPTDLLIEQGDSTPMTLGTNPIRSVQATRSFLQKARPLYR
                     HMLHTAAEACRGSDLLIHGLPTIWGAHIAEGLGIPAVRANLQPLTPTREFPSALLPFR
                     SSLSGIGNWLSHWAVTQ"
BASE COUNT         2410 a         2418 c         2259 g         2321 t
ORIGIN      
        1 tttcgatggc ggcggcaagg gcgagttctt ggttgcgccc gcctttgcca tgtccacgca
       61 gggtgactgt cgtttcaccg cctgcaatga gacaaaatgg acgttcgtaa ttgaggttga
      121 aatgtccgca aagttctttt gcaacgtcac gggcttcgcc ttgccacgta tcaccgagga
      181 aatgagtatg gaaaccaaga gattttgcca tctccaaccc tgcttgagcg gcaagggaat
      241 tgcttccaac tagaacattc tgcactcgct caaacaatgg gtcattcggt tttggcgttt
      301 cgggtgcggt ttctaaaact cccaaaatta ccactgggac tttatcgagc aagtcatatt
      361 tttccaatat cgtcagcgca tcttcgcgag tggatgggtc tggcgcggtg ggaccggagg
      421 caatcgcttc gagcggatta ccgacaacat ctgaaagaat caggctggca atccgcgctc
      481 cattcgcaga cttggcgatg ccgccaccct tcgctcggtc gaggtgacgg cgcagggtgt
      541 tgatctcgtc cacgcgggcg ccacaggcga ggagaagttt ggtgagggtt tgcaggtctt
      601 caagctgaag cccatgttgc ggagaagtca tcaacgcgga gccgccgcca gagatgaggc
      661 agatgagcaa gtcatgttcg gtgagcgacg aagcgaggtc gaaggcggca ttgcccgcgc
      721 ggacactggc ttcggttggg acgggatgtc cgccgggtaa gacggcgaat ccactggcag
      781 gggaagcaaa ggcttgtttt ggaatgagca agccgcggct gggatatgag ccgagtacat
      841 ccaacaaggc aagtgacatg gcttctgtcg ctttgcccaa gccaagtacg gtcacattgc
      901 caaactcatt caaatcatat tttttattat caatgaataa taaattcttt tcgcgctgga
      961 caaaacgtcg aatggcattg cctggttcga cggcatcgat gcaggcattt agaatctgca
     1021 tgatatcgtt tctgcgggga tgagtttgca gcgtgacagt ggaaaaattg cgcatgtttc
     1081 gagtatgata acgcagaagg agatgcaatg cgacccaagg tatttgtttc gcgcaagatc
     1141 cgtgatgcag gtttgaattt ggtgcttgag ttttgcgatg cggaggtctg ggatgaagag
     1201 atgccgccca cgcgcgatgt gctgcttgaa aaagtgcgcg gtgtggacgg ctacttgagc
     1261 atgctcacag accgcgtgga tgcagaatta ttggatacgg ctggaccgca gttgaaagtg
     1321 gtcagcaatc acgcggtggg atttgataat gtcgttgtgg cagatgcaac ggcgagagga
     1381 attccggttg gaaacacgcc gggtattttg accgatgcca ctgctgatat tgcatttgct
     1441 ttgttgctcg cctcagcacg acgaattgta gagtctgaaa aatatatccg cgatgggaag
     1501 tggaagacgt ggcatccgtc tatgttgttg ggcatggacc ttgcggaaag gacgctgggc
     1561 ttggtgggct ttggtcgcat tgggcgggcg gtggcaaagc gtgcggttgg ttttggaatg
     1621 cgtattttgt attacagccc aaactcagaa cccgaatttg gcgcgatcaa aatgaacagc
     1681 ctcgatgcgc tgttacaaga atctgatttt gtttcggtgc atacaccctt gaacgagtcg
     1741 acgcgccatt tggtaaacgc ggactttctc tcaaagatga agccgaatgc ggtcttcgtc
     1801 aacacctcac gcggaccagt ggtagaccaa gtcgctttgt atgatgcatt gaaggcaaag
     1861 aaaattttcg cagcaggttt ggatgtgacc gaccccgaac ctttgccgtt ggattcgcct
     1921 ttactaacct tggataactg tgtgatcgtt ccgcacattg gcagcgccag cgagcggacg
     1981 cgcgatgcga tggcaaggct cgccgcagaa aatctcatcg ccgggttgaa aggcgaacgt
     2041 ttgccacact gtattaatcc tgaagtatac gaaaatagac gatagaccac ggacgataga
     2101 aagttccatg gtcaatagtc tatcgtccat cgtctaaaca aggtaaaact ctctcacttc
     2161 acttaatctg aggctacatg aatcgcacaa agaacgcacg agtttgcatt atcggagcag
     2221 gtccctctgg cattacggct gggaaaaatc tcattcaggc aggtgtcacc aatttcgtca
     2281 tctatgaaaa gaacgaccaa gtcggcggga attgggtctt caatgacaag accaaacact
     2341 cgtctgtgta cgaaacgacg cacctcatca gctcgcggcg ttattcggaa tattttgatt
     2401 accccatgcc cgcagactat cccgattacc cgtcgcacac gcagatgctg gcatattttc
     2461 aggggtacgc caaacacttt ggtgtgatgc cttatattca attcaacacc gaagtccaca
     2521 acgctgaaga attaccagat aaaacttggc gcatcacact tgggaatagc aaagtagaaa
     2581 tctttgactt tctactcatc gcgaatggac atcattggaa tccacgttat ccaaagtatg
     2641 aaggtgagtt cacgggagag ttcatccact ctcatgaata caaacgcgcc gcaccattta
     2701 cagggaagcg cgtgctcgtt attggagcag gcaactctgg ctgtgacatt tcggtagaaa
     2761 ccgcgcgggt ctcgacgttc accgccatgt ccatgcgacg cgggcaatac atcgtgccta
     2821 agttcgtttt tggtcagccg cctgatgtgg tcaatggtcg tttttactgg ctgcccgcgt
     2881 ggattcgcct gcccttcttt tatctaaccc tcaaacttgc tgtcggtaaa tacgaagatt
     2941 atggcttgcc caaacccaaa cacggcatct tgaacgcgca cgtgaccaac aactcagaaa
     3001 ttctctacgc catccgtcac gggaaagttc acgtccgccc agatatcaaa cgcttcaacg
     3061 gcaaacaagt ggagtttgtg gatgggcgta tggaagaata tgactcgatc attgccgcta
     3121 ccggatttaa gatctcgctt cctttctttg accccaacct gctctccatc gaagaaggtg
     3181 ttcctccgct gtataagaat gttttccatc ccgaccaccc atcgctattc tttatcggtc
     3241 tcgtgcaacc gctcggctcg atctggccac tctcagacct gcacgccaaa cttgccgcta
     3301 attatattat cggcaattac cacctaccca aagatatacg tcaacgcatt gcgcgccata
     3361 ttgaatatat gaaaaaacat tacgtcaatt cgccgcgcca cagcgtggaa gtggagtttc
     3421 atccgcacat tgtggagcta aagcgcgaga ttcccaagtc tgcgccggaa tggaagcatg
     3481 gatattcaga tcgataacct tgtgcgtgcc aaacgccgca ccattgccct catcgtcgaa
     3541 cgagatggta gccttaccgt ccgtgcgccg aggcgggcta cactgaatga tatctatggc
     3601 ttcatccaag aaaaaaccga ttggatccta cgttcgcgcg aaaaactaaa agcgataaag
     3661 cccatcccga aaaaagcata tgtggatgga gaacgtttcc tctttcttgg gcaggagtac
     3721 gaactccggc tcgttccacc tcagcggcct gccctcaaat tcgacggtgg attcaccctg
     3781 tcaacttctg cacaagaacg cggtgaacta gccttcacta aatggtacaa aacccaggca
     3841 ctgaccctct tcacggaacg tgtgaactat tacgccaacc tacacggact tatcccgaaa
     3901 gaggtcaaag tcaactcggc aaagacacgt tggggttctt gtacatccgc cggcacgctc
     3961 aacttcacct ggcgcttggt catggcgcca ctcgaggtga tcgattatgt cgtgctccat
     4021 gagttggcgc atttgaaggt caaaaatcat tccccgcgat tttggaagtt ggtagaatcg
     4081 ctctgccctg actttaaaca tcaccgcaag tggttacgcg accacggcga aacaatgacc
     4141 ttataacttt tctctggaga atagccatgc tgcccgaaac tctcatagaa gtctacgaac
     4201 ataccgaacc agattacaaa cctcttgtcg attatcagtc ttggcgagtg gcactgatga
     4261 actatacaga cgacctaacc cccgagaaca tcaatcgcat gcaaaagcat acagaaaccg
     4321 acgaggtctt tgtgcttatg gctgggcatt gtattttatt tctcggcgaa ggcgaagaga
     4381 cggtaaccaa agtccacgcc gtggatatgg agttgtacaa actttataac gttaagcgtg
     4441 gcgtatggca ttcgcatacc ttcagcaaag atgcccgcat tttgattgtg gagaatcgcg
     4501 atactgttgt ggaaaattcg ccctttgtgg atctatcgcc aagtcaatgc acagaagtgc
     4561 tggaattaac aaaacaatta tggaagtaaa acgtaagagc gaggaaaccc cgcccttacg
     4621 tttttaccca aactcaaaag tgggcatgcc ctcatatttt gtgtcaactt tgaacaaccc
     4681 gccagagagc gggtatttat ttaagtccgc ttcgctcatg cctttgcgag cagaggtgat
     4741 atacaactca ttacggttct tgccaccaaa gaccgggcag gaagagtgca aagcaggcac
     4801 agaaacctgc tccaagagtt gaccggtatt cgggttccag cgtgtgacct gtgccccacc
     4861 ccacatggcg atccatagat tgccttcaac atcagaggtc atgccgtctg cccagccaag
     4921 ggatttggga acctcaaaag cacgccggcg gttcgcgatt tcaccggtct tgatatcata
     4981 atcataggcg cggacttcgc aggcgggcgt atcaatgtaa tagaaggttt tatagtctgg
     5041 gctccatgcc atgccattgg agatggtcac atcacggaac aaaacacggg tggattttcc
     5101 atcataggaa tagacagagc ctgtggggtc cgtctcgttc aaatccatgg tgcctgcaat
     5161 caagcgccca gatgggtcac atttgccatc gttcatacgg ttggtggcag attcattcaa
     5221 agtgacaagc acagtctgct gggaggtggc aatgtccaag tccacgaaag aggcgcgttt
     5281 gcctacgatg agatgtccgc ttttagtagg ggcaacaaat ccaacatatt catcaaattg
     5341 agcgagaatt tcgttacctg catagactct tttttcaaaa atatcaaccc aatacaaggt
     5401 ttgagttttt gcatcccaag atggaccttc gcccaagttt gattttgcgt caaaaaaaag
     5461 ttcagctttc attagaacca attcctttta tagaagatat aagtggccaa tgccgtgagc
     5521 gccaacgaca agccaataac cgttaagaat gccagcgggt gtccttctcc tggcaaatcc
     5581 acattcatgc cgtaaaacga tgcaacgatg gtgggcatat tgagaataat cgtcagtgcc
     5641 gcaagcgctt tcatcactac gtttaagtta ttcgagataa tcgaagcgaa ggcatccatc
     5701 ataccagaga gaatttcagt agcgatgctt gtcatctgaa tcgcttgctg attttccgtg
     5761 agcacatctt cgagcaagtc ttgatcttct tcataataat tgaagagttg agtcttttgt
     5821 acgcgttcca tcatcacttc gttagagcgc aacgcggtcg caaagtaggt aaggcttttt
     5881 tgatacttga gcaattccaa cacttcacgg ttttgcgtgg atttttgcaa acggtcttcc
     5941 acggcttcgg tagcgcgatt aatctcacgc aacaagttca aataacgcac agctgtttcg
     6001 agaaagatgt aaagcgtgag gcgataacgc ttgccagttt tcaaatagcg atgtttacca
     6061 ttggttaatg cgcgaaagat atcactatcg aagcggcaaa ctgtgatgat cttattgcca
     6121 agaatcataa tgccaagcgg gacggtgtta taaggaatat cgctctctgg ttggtgaatg
     6181 ggaatgcgaa gaaggatgaa ggtgtaatct tcatcgcgtt ccatacgcgc catttcatct
     6241 tgatcaagtg aataattgat atagtccatc tccatccccc aacctactaa cttttccatt
     6301 tcatccggcg ttgggtcaat cacattaacc cacgttccat tcgcgatcgc atcgagcttc
     6361 tctagaccgt tctcggtcgt tttgtagata ctcagcataa cagcctccta cgtggaatca
     6421 tcacgtaagg gcatggcacc tacgtgaaag gtattaaaac gttaaataca tgttcaccgc
     6481 tacttagcgg gttactatct gaagaaccta catcgctttc agtggttaga tacatacttt
     6541 tgaaccgcgc caattttaag ccggatgatg gaattacaca acagtttcag gatttgactt
     6601 tgggtttgtg cccatagacc gcaaacacgc aatgcgaaac atacaagacg aaatcatccc
     6661 gcgcctcttc cttgacctgt ctctgcttca acccttcaag cagactcttc aactgcgagt
     6721 caaatctcac tatttcgtct gccgaaaggc tgccacccac cccagcactc gcacggatgc
     6781 gtccacgcca ggcttcggct gtgtagggca catggatatc gaatgagaat gaagtgacat
     6841 cttcaaagcg cgcttctcgc aagtcacgaa accaatcggg gtagctaccg ttcttattcc
     6901 caaagcgttc ataccatttc gggttataga tttggataag cctctccgtt cggtcgacaa
     6961 cattcccagg cagcggaagc cagtcgaagt gagcgatcaa cactctgccg cccggcttaa
     7021 gcaaacgctt cacttcctgc gctgctcgcg gacggtcgaa ccaatgccag cattgcccgg
     7081 cagagacgac atcgaatgac gcatcaggta acccggtttc ttcagccttt cccaaacgaa
     7141 actcgatacc caaaccttgt tgaaggctga ggtcttttgc ctgctcaagc aattgtgaag
     7201 aaatatccat gccaatgacc gaacagcctc gagcggcgaa tccgcgggcg agggtgccag
     7261 tgcccgttcc gaggtccagc aaagaggcgg tggggtttac gatcccatca gcaaagacgc
     7321 ggtcaaaaaa ttcatcaggg aatccagccc ggtgtttggc ataatcttga gctgtaagac
     7381 caaagttata ttccatagac tatatttttt tcaaatacat cagtgcttta aacaaaaccg
     7441 ccacatgcaa agcatgcttg aatgtgcctt gccgcaccat ttcgatcaca tcatccaatg
     7501 gcataagcac aacttcgatc tcttcggtct cgtcaaaatg ctgttcacca ttcggttcga
     7561 catcggttgc aagatagcaa aaaatggtat tctcttgaat ggcagggttg gggtagatct
     7621 gcccaacttc aataattttc tctgcagaat aacctgtctc ttccattagc tcacgtcgcg
     7681 ccccgtccag cggactctca ccatcattta ccacgccgcc gggcagttca agcaatgttt
     7741 cttgcacgcc atgccgatac tgccttacca aaaccacttc gttgtttttg gttaaagcca
     7801 atacattcgc ccagctacga aattcaaaga cgatcggctc aaaggttgta ccattgtgaa
     7861 cctcaaccgt atccacccga aaacggggat atatgtgccg cgaactcaaa atcttccaag
     7921 gggtaatgga catgttttac acagcctctt tattgcatag tttttaactt ctcaatcaaa
     7981 ccattataat ttccctatgc ctattactac tgactccgtc cgcacgtatt acgatgaaaa
     8041 cacaaacctc ttcttgaaat ttagcggtca caaaaaagct cagaacattc accgctcgtt
     8101 gtggatgcca gataccaagt cgcttgaaga tgcactcaac acctccaatg cgctcatcaa
     8161 agcggagatc gaatctgtgt ctcaaacaaa cgcccgcatt gccgatctcg gctgcggtgt
     8221 gggagcgagt ctgttctata tttatccgcg tttgcaaaat ccatcccctg ccctcggatt
     8281 aactcttagc cccgtgcagg cacggctggc acaagagtcg gcaagtcaac tcggcttgca
     8341 agatcacatc cgcttcgccg aaggggattt cacttcggta ccactgccga gtgattcact
     8401 tgatgccgtc tattccgttg aggcggtttg tcacgccgtg gatcctgaaa agtatttcaa
     8461 agaagcgggg cgcctgctgc gcaagggcgg caagctcatt ttggtggatg attacaaatc
     8521 agcgcgttcc ttcaactcaa gtgaacaaaa atggttcgat gcttacattg atggttggta
     8581 tgtccccggt gtgcgtactg taaagcaaac gattgacttc gcaaacaagt acggacttca
     8641 actcaaaaag aacgacgaac ttacgcctca tcttcgtcta cgcaacctgc ccgattttct
     8701 cgccagtgcc ttgctcttcc tcggtgagaa acttccgatc aagcacgcca ttgtcccgtc
     8761 catgttgggg agcatggcat tgcaacagtg tttatatatg aaggtggctg aatatcggat
     8821 gatggtgttt gaaaaaatcg cgtaagggcg aggtgacctc gcccctacaa caacgaatgc
     8881 caaaaactat tactctcctc accagtggca cactcggaga tgtcctccca tacatcgcac
     8941 ttggcaaagg gttgatcgaa gcagggtaca acgtccgtgt ggctgcgccg cgcgggtttg
     9001 cttatctctt caacgccgag ggcgggggga ccccgcccct acaatttact ccattcgagg
     9061 ggaatcccac cgatctgctc atcgaacaag gcgactccac gccgatgact ctcggaacaa
     9121 atccaattcg ttccgttcaa gccacgcgca gcttcttgca aaaagcccgc ccgctctacc
     9181 gtcacatgct gcacaccgcc gccgaagcct gccgcggttc cgacctgctc attcacggct
     9241 tgcccaccat ctggggcgca cacattgccg aaggcttggg cattcccgcc gtccgtgcca
     9301 acttgcaacc gctcacacct actcgcgaat ttccatctgc ccttctgcca tttcgttctt
     9361 ctttatcagg gatcgggaac tggctctcgc attgggctgt gactcaag
//
LOCUS       DAOQJE010000016         9370 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_3892_length_9370_cov_5.046592, whole genome shotgun sequence.
ACCESSION   DAOQJE010000016 DAOQJE010000000
VERSION     DAOQJE010000016.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 9370)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9370)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9370
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3892_length_9370_cov_5.046592"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..358
                     /locus_tag="PLT08_00775"
     CDS             <1..358
                     /locus_tag="PLT08_00775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE03021.1"
                     /translation="EIKILEIFSVFIKNLNFQLSPKFRDLRHLGEMSEGQRGLSSQLS
                     LKRPIQHGRHQHGQLPALDLLIDPEHSERERIDLRLQYNFFVPPNEKPPPDNLHGHSG
                     GGTIFFNSSIKGSNFF"
     gene            complement(426..743)
                     /locus_tag="PLT08_00780"
     CDS             complement(426..743)
                     /locus_tag="PLT08_00780"
                     /inference="COORDINATES: protein motif:HMM:NF037100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HNE03022.1"
                     /translation="MNKLKIKVPKAKIAEFCKRWNISEFAVFGSALTADFRPDSDVDV
                     LVSFDPQTQVTLFDMVHMQEELRTIFGREVDLVSKRGVMNSRNYLRRKNILESAQVIH
                     VAR"
     gene            complement(843..1100)
                     /locus_tag="PLT08_00785"
     CDS             complement(843..1100)
                     /locus_tag="PLT08_00785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE03023.1"
                     /translation="MVGSLILLLGSFGKASLKKYGRIADWSILGLVFGWLFQISMAVT
                     GTTPERIGVVWPMSALMVYALLGVSVLMVAGRWLIRRVLGR"
     gene            complement(1160..2227)
                     /locus_tag="PLT08_00790"
     CDS             complement(1160..2227)
                     /locus_tag="PLT08_00790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE03024.1"
                     /translation="MDTYPNINLDKYYFIDQGNLFDRFNWWITLTLMDPIMPLSVYRP
                     TIYKKKRTIWKLPRFRDVAKLIGLYNMESDSYLGWHNRRLGIFMTLLFFSEVAISLSL
                     IMPFAYGLPGLMRILLLLSGNINLTITLLLAFCLLLLTYRIAFTMSTLLLERKFAETL
                     CIMQITYILLELTRQDVIRSRKHDLLNRINFLANKLILMGMNYAGNDEVNKKWAFDLF
                     NKMSRFVRERERLVIAPTENSLEQLRQDFFRLGKIIASGNYGSFEFEAGDVEITDQRP
                     KSLSSQIFIIAIRVLSVAIPIFILIFMFNSTEAFDQLNINRDSVFYISIAWLLFALDD
                     LIGIGIIEKFLNLVKVSKELR"
     gene            complement(2430..2894)
                     /locus_tag="PLT08_00795"
     CDS             complement(2430..2894)
                     /locus_tag="PLT08_00795"
                     /inference="COORDINATES: protein motif:HMM:NF019844.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0070064 - proline-rich region binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HNE03025.1"
                     /translation="MATGKTKFSNYETITLVLAILTIVVTIIGFFISGHDWFPAPLPQ
                     TETSVAISTSSNSDNVTATSNLCEGYNSRVQIGAKVITANALDQSLNIRKLPSLSSPI
                     VNSLQNNAELQILDGPICSDKIMWWEASTTDGKVKGWVAEVSLSGVYYLRPE"
     gene            complement(3077..3886)
                     /locus_tag="PLT08_00800"
     CDS             complement(3077..3886)
                     /locus_tag="PLT08_00800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE03026.1"
                     /translation="MTFPQILEVAIGLVLTYYIMGSVVSAVTQYALDTLRIKRKIFEE
                     YMGLIFGKRDVKRILALPQVKSLGTKVTVNILVDSFWDIFGFTAYERSPSATEEMLIR
                     LVNMIPEDSAGKQAMVTWINCAITNTNDLRVRITDYFAGLKEEIEANYSSKSRKYMVI
                     ISLAITIITGVDSLQLAKELWTNSELRTLAMQSANIAASGSSESATASSPADITTIPI
                     KIGWVANDFPSNPSFGIWLSYLLYKIAGLGVTVACVSQGSAFWSGLLKKLG"
     gene            complement(3938..4174)
                     /locus_tag="PLT08_00805"
     CDS             complement(3938..4174)
                     /locus_tag="PLT08_00805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE03027.1"
                     /translation="MMILKIQDEQRRIVAYLDGLLAKVNALRESVLRDDVREPRKGVI
                     LQVKGGAPKGCDKGLSALMVSVLDRAFKGSSNYI"
     gene            complement(4199..4789)
                     /locus_tag="PLT08_00810"
     CDS             complement(4199..4789)
                     /locus_tag="PLT08_00810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE03028.1"
                     /translation="MTDPQIDFWNFILSAVSTFVVVSAALAGTIKYFQERQRDREVRR
                     EELSWRKTQFLLELADDFEEDERHEPAWQFLAYGIGLPKNSSLKKILGGNVNILTASE
                     LKLRYSIDNYLDFFDRLYHFTFITQSLNISDIEVFGWYIAQVGETKEIYEYAKNAGFE
                     DVLKLNKELQKLFGKRHWYKTIRKHRTINKSKVTKS"
     gene            complement(5030..5728)
                     /locus_tag="PLT08_00815"
     CDS             complement(5030..5728)
                     /locus_tag="PLT08_00815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE03029.1"
                     /translation="MSNPLSRLFQQWVEYRGRNAFWDTPTFDKDQVISRPDYLKVSSI
                     IEGAYIYNFPATCPILVSTDGKKIRGTGGYLPLKYGGYYLYYIDIREKTFSIPELLTI
                     TLDSVQLNIKASFKYQVKTPELIFDIQEPVQTLQKDFINALQHFFQGRSFQSLSSPAL
                     SEELLRKVVPYLPTRYFVISNLSVSFYKVGAKNISNIETPAPPEKIINIFIGGDISGT
                     LTIGDSNNVSNKSK"
     gene            complement(5764..6681)
                     /locus_tag="PLT08_00820"
     CDS             complement(5764..6681)
                     /locus_tag="PLT08_00820"
                     /inference="COORDINATES: protein motif:HMM:NF039496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5655 domain-containing protein"
                     /protein_id="HNE03030.1"
                     /translation="MSDIKLFNTKGNQVEVIKGHSVAIEKSLQTLIENNLEIFLGIRL
                     VDSEHSTGKTHGGRIDTLGIDENDCPVIIEYKRSLNENVINQGLYYLDWLLEYRAEFV
                     LLAQKIDPKLTSEDINWDGARLICIAGEFTKFDEHAIKQIDHNIELIRYRRYGEEFLL
                     FELVNASFEEYSEKAITSKPKSVSKSIESNVLEKLGKANTEVKDRYEALKVYCQSLGD
                     DVQVKALKHYIAFKRIKNFAAVEIHPQDKNIIVFVKVDFNSVLLEEGFTRDVRKIGHF
                     GTGDLEITIRSDEDLEKAKPLIVKSYEDN"
     gene            complement(6692..7498)
                     /locus_tag="PLT08_00825"
     CDS             complement(6692..7498)
                     /locus_tag="PLT08_00825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE03031.1"
                     /translation="MPSKKKTSVTIQAAIITVVGTGFFALITSVLTPVIQHWLEKPTE
                     STSDENSNIDPLIEYFPLFVGSTRTYTVGNSTPTTDGMEGLIEYISTYTEKVIFVESG
                     AYEKIHIYKVEQSGEIYDLDCTTWETRQGPVNKWYITFENKLYTACSEDGKVAILQSL
                     YEEIKSPDKTDKITPKIIFPIEIGNSWGAITAPGEDHMYKYNVEIKTSLSTPAGSFTD
                     CYKIVFYTSGDSSIRYVCPGVGDVALEYHHYGNPINYRVELQSYSNADNK"
     gene            complement(7510..7887)
                     /locus_tag="PLT08_00830"
     CDS             complement(7510..7887)
                     /locus_tag="PLT08_00830"
                     /inference="COORDINATES: protein motif:HMM:NF039390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE03032.1"
                     /translation="MDGKSLDLTAEKLAQLCDLFPEVFSEGKVDFARDCEADESIKFF
                     FKLPRDFKVPTPIGNYRPDWAVVFENDKRIYFVAETKGTINWQELREVEKMKIKCGTK
                     HFAVFKESGVEYKLAVTTRDLYS"
     gene            complement(7931..8803)
                     /locus_tag="PLT08_00835"
     CDS             complement(7931..8803)
                     /locus_tag="PLT08_00835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE03033.1"
                     /translation="MDLRDPIWQGFEAIVTITGVIFTIIFFIAPEAREWLIRTIRSSA
                     FGLLLGGLFILCLCAAFLIPVTKGVASLLARPTPTNTAIPLPTIFPTNTLNPSTPTPT
                     YTYLVIDDFNTGISDSWQIKSGQWQMVNNRLTIINVLDGTDWSKGKIMTGDQTRGNYE
                     VGVDVHLVNIETRINIAHIIVRAIDDSNYLLLEIRDFDLFDSDWSRGSWYIVSNNELT
                     EISNAGFSSFLTTDFSINIQLQNDIVTTFIDGNQVSRWSGAPYPNGMVGLEVASRYPT
                     DPAAFDNFYIRTQP"
     gene            complement(8814..>9370)
                     /locus_tag="PLT08_00840"
     CDS             complement(8814..>9370)
                     /locus_tag="PLT08_00840"
                     /inference="COORDINATES: protein motif:HMM:NF022337.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF2726 domain-containing protein"
                     /protein_id="HNE03034.1"
                     /translation="GKKDSILTPAESRFYRALLEALENRAVIVPKVGLQDVFDILDRE
                     QYRKARNRISTRHIDFLICEIGTLRPLFGIELDDSSHNKPEAQRRDELKNRVFAAAML
                     PLVRIPVTGTFKVEFIQEKIQPFFEKEEKKEGPSEAPLCPNCKIPMVRKTAQRGEHKG
                     QDFWACPNYPTCKELINISQNSSN"
BASE COUNT         2571 a         2144 c         1852 g         2803 t
ORIGIN      
        1 tgaaatcaaa attttagaaa tattctcagt gttcataaaa aatctcaatt tccaactctc
       61 ccccaaattc cgtgatcttc ggcatttggg ggagatgtcc gaaggacaga gggggctgtc
      121 ctcacaactc tcccttaaac gccctatcca gcacggacgc catcagcacg gacaactccc
      181 cgcccttgac ctgctaatcg accccgagca cagtgagcgg gaacgcattg accttcgcct
      241 gcaatataac ttcttcgttc cgccgaacga aaaacctcct cctgataacc tgcatggtca
      301 ttcaggagga ggaacgatct tcttcaactc ttcgatcaaa ggcagcaatt tcttctgaac
      361 cgtgtcccac acctgattga tgtccactac atcatattcg tggatcacca ggtttctcat
      421 actgatcatc gcgcgacatg gatcacctgc gccgattcga ggatgttttt ccgcctgaga
      481 taattgcggc tgttcatcac gccgcgcttg ctcaccaaat cgacctcgcg cccaaaaata
      541 gttctcaatt cttcctgcat gtgaaccatg tcaaacaggg ttacttgtgt ctgtgggtca
      601 aacgagacca gcacatccac atcactatcg gggcgaaagt cagcagtcaa agccgaacca
      661 aaaacggcaa actcggaaat attccagcgt ttgcagaact cggcgatttt cgctttcggg
      721 actttgatct tcaacttgtt catgttgtaa ttatagcaaa tatgtcattg cgaggaggat
      781 gcactttccg atatcatccc gaatggtttt atcgggacga agcaatctcc tcgcgcctga
      841 ggtcacctcc ccagcacccg gcggattaac caccgtcctg ccaccatcag cacggacaca
      901 cccagcagtg catacaccat cagcgcggac atcggccaga ccaccccgat gcgttccggc
      961 gtcgtaccgg tgaccgccat gctgatctga aacagccagc cgaagaccag ccccagaatg
     1021 ctccaatccg caatgcgacc gtacttcttc agcgacgcct tgccgaacga ccccagtaat
     1081 aagatcagac ttcctaccac gagataagcg acaatgtgcc gctgttcgtc aagtaggata
     1141 cgatgaaata gaggctacat catcgtaact ctttactcac tttaactaag ttcaagaatt
     1201 tttctataat ccctattcca ataagatcgt ccaaggcgaa caatagccaa gctatcgaaa
     1261 tatagaaaac agaatctcga ttgatattca actggtcaaa cgcttcagtt gaattaaaca
     1321 taaagatcaa tataaaaata ggtatggcaa ctgacaatac tcttatcgca ataataaata
     1381 tttgagacga taaagattta ggtctctgat ctgttatttc aacatcacca gcctcaaatt
     1441 caaaagaacc gtaatttcca cttgcgatta tctttccaag acgaaaaaag tcttgacgga
     1501 gttgctcaag cgaattctca gtgggcgcaa taactaatct ttcgcgctcg cggacaaacc
     1561 ggctcatttt gttgaaaagg tcaaaagccc attttttatt gacttcatcg ttccctgcat
     1621 aattcatccc cattaatata agtttgtttg caagaaaatt tatacgattg agtaagtcgt
     1681 gttttctact ccggataaca tcctgtcggg ttagttcaag caaaatataa gtaatttgca
     1741 taatacataa tgtttcggca aatttccttt cgagtagcaa agtagacatc gtaaaagcta
     1801 tccgataggt caagaggagt aaacaaaaag cgagaagaag tgtgattgtt aagttaatgt
     1861 ttcctgatag aagtaacaat attcgcataa gtccaggaag cccataagca aagggcataa
     1921 tcaaacttaa agatattgcg acttctgaga aaaacaaaag ggtcatgaaa ataccaagcc
     1981 ttcgattgtg ccaaccaaga tacgaatccg attccatgtt atataaaccg ataagttttg
     2041 ctacatctct aaaccttggt agtttccata tcgttctttt tttcttgtag atcgtagggc
     2101 gataaacact caacggcatg attggatcca tcagtgtcaa cgtaatccac caattaaatc
     2161 ggtcgaatag atttccttga tcaatgaaat aatacttgtc cagattaata ttgggatatg
     2221 tgtccattat gtactcctaa attatatttt aaatacatct cgtaactacc cataaaacac
     2281 cctatccagc acggacgcca tcccgcaagg ggacgttgtc aacgtccgcc caaagagcga
     2341 gcgcaactcc tccccgccct tgacctgcaa ctcccgcaac gcattgacct tcggtggtaa
     2401 gaagagaaca attagagcag gtgtcatcat tattcaggtc gaaggtaata gactccactt
     2461 aaacttactt ctgcaaccca tcctttaact ttcccatcag tagttgaagc ctcccaccac
     2521 atgatcttat cagagcaaat tggaccatcc aatatctgaa gttctgcatt gttttgaagt
     2581 gaatttacaa taggcgagga aagactaggt agttttcgaa tattgaggct ttgatccaga
     2641 gcattggcag taatcacttt tgcacctatc tgaactcttg aattatagcc ttcacagagg
     2701 tttgatgtcg cagttacatt atcagaattt gatgaagtgg atatagcaac actagtttct
     2761 gtttgaggta gcggagcagg gaaccagtca tggccagaga taaaaaaacc aataattgtc
     2821 actacaatgg tcaggatagc gagaaccagt gtaatagttt cgtaattaga aaactttgtt
     2881 ttacctgtag ccatcgcaca actctccctt aaacgcctta tccagcacgg acgccatcag
     2941 cacggacaac tccttatcac accctttggg tgccccaccc ttgacctgca atatcacccc
     3001 cttgtggggc tcccgcacat cgtccccgag cacagcgagc gggaacgcat tgaccttcgc
     3061 gatcattgct ttgatcctac cccagtttct ttaacaaacc agaccaaaat gctgatccct
     3121 gagaaacaca cgcaacggta acccccaatc ctgcgatttt atacaaaagg taacttaacc
     3181 atatgccaaa agatggattg gaagggaaat cattggctac ccatcctatt tttattggta
     3241 tggttgttat gtctgccggg gaagatgcgg tagcgctttc gctactacca cttgctgcaa
     3301 tattggcaga ttgcatcgct aatgttctta attctgaatt tgtccacaat tccttcgcca
     3361 gttgaagact gtcaaccccg gttattatag taattgctaa agaaataata accatatatt
     3421 ttcgtgattt tgaagaatag ttggcttcta tctcttcctt cagaccagca aaataatcgg
     3481 taatccttac tcgaagatca tttgtatttg tgatggcaca attaatccat gtgaccatgg
     3541 cttgttttcc cgctgagtct tctggtatca tatttactag ccgtattagc atttcttctg
     3601 tggccgaagg gcttcgctca taggcagtaa agccgaaaat gtcccaaaag gaatccacta
     3661 atatattaac agttaccttc gttccgagag atttaacttg gggtagggca agaattcttt
     3721 ttacatctct tttgccgaaa ataagcccca tgtattcctc aaatattttt ctttttattc
     3781 gaagagtgtc taaagcgtat tgggttacag cggagaccac agaccccata atgtaatatg
     3841 tgagcaccaa gccaattgct acttccagaa tttgcgggaa agtcatgatg atctccttat
     3901 ggggcaaata gatttcttgc ggtccaaaca ccttctgtta tatgtaatta gaacttcctt
     3961 tgaacgccct atccagcacg gacaccatca acgcggacaa ccccttatca caccctttgg
     4021 gtgccccgcc tttgacctgc aatatcaccc ccttgcgggg ctcccgcaca tcgtccctga
     4081 gcacagactc ccgcaacgca ttgaccttcg ccagcaaccc atccaggtag gccacgatgc
     4141 gccgctgttc gtcttggatt ttcaatatca tcattcttcc ttacgcctga atctgatact
     4201 aactttttgt aactttagat ttattgatgg ttcgatgttt gcgaatagtt ttataccaat
     4261 gccttttgcc gaatagtttt tgaagttctt tgttcaattt caaaacatct tcaaagcccg
     4321 catttttggc atattcataa atctctttgg tttcgccaac ttgtgcaata taccatccaa
     4381 agacttcaat atcagatatg tttagagatt gggtaataaa ggtgaagtgg taaagtcgat
     4441 caaagaaatc aaggtaatta tcaattgaat aacgaagttt cagttctgac gcagtaagta
     4501 tgtttacgtt tccccccaat atctttttta acgaactgtt ctttgggaga ccaataccat
     4561 acgctagaaa ttgccatgca ggttcatggc gttcatcttc ttcaaaatca tccgccaatt
     4621 caagcaaaaa ttgagttttg cgccaagata attcctctcg tctaacttca cggtctcgtt
     4681 gtcgctcttg aaaatattta attgtgccag caagagcagc actcactaca acaaacgtac
     4741 taacagccga aagaatgaaa ttccagaaat ctatctgcgg atcagtcata tgtactccct
     4801 tttggtaaac tccttattga aaaggagcga taagccaaat gaattgaaac attctcacaa
     4861 ctctcctttg aacgctctat ccagcacgga ctcccgcgcc acattgacct tcgcacccga
     4921 agaccatcca cgaatggacc acgaatgcac ggatacgccg ccatccattc gtgtacgtgt
     4981 gcccgttagg gtattcgcgg acggtcatca taaaaatgca aatccaatat tatttggatt
     5041 tgttgctaac attattgcta tctccgatgg ttagggttcc actaatatcc cctccaataa
     5101 agatattaat gattttttcc ggaggcgccg gggtttcaat attggagatg ttctttgcgc
     5161 caaccttgta aaaagaaacg cttaaatttg atatgacgaa atatcttgtt ggtagataag
     5221 gtacgacctt ccttaatagt tcttcagata atgcaggaga actaaggctt tgaaaacttc
     5281 tcccttggaa aaaatgttgc agggcattga taaagtcctt ttgcagtgtt tgtacaggct
     5341 cttgaatatc aaaaatcaat tcaggcgttt tgacttggta tttgaagctt gctttaatat
     5401 tcaattggac actgtctaaa gtgattgtga gaagttcggg gattgagaat gttttctctc
     5461 ttatatcgat gtaataaaga taataccctc catatttcaa gggaagatac ccccctgttc
     5521 cgcgtatttt tttgccatcg gttgaaacaa ggatagggca agtcgctgga aaattataga
     5581 tgtacgcccc ctctatgatt gaactcactt ttaaataatc cggtctgcta atgacctgat
     5641 ctttatcaaa tgtaggagta tcccaaaagg cgtttcttcc cctgtattca acccattgct
     5701 gaaataacct gctaagaggg tttgacattg attgctccta tataaatgct gaagtcctgc
     5761 tttttaatta tcttcgtaac tctttacgat caatggcttt gccttttcca aatcctcatc
     5821 tgagcggatg gtaatctcca aatcaccagt tccaaaatgc ccgattttcc gcacatctcg
     5881 tgtaaagcct tcttcaagaa ggacactatt gaagtcgact tttacaaaca caataatgtt
     5941 cttgtcctgc gggtggattt ctacagccgc gaagtttttg atgcgtttga aggcaatata
     6001 gtgtttaagc gctttgacct gcacgtcatc cccgagtgat tggcagtaga cttttagagc
     6061 ctcgtaacgg tctttgactt ctgtatttgc tttccccaat ttttctagta cattgctttc
     6121 gatggattta ctgacagatt tcggcttgga agttattgcc ttttcgctat attcttcaaa
     6181 cgaagcattg accaattcaa ataacaggaa ctcttcccca taccgtcggt atcggattaa
     6241 ctcaatgttg tggtcgatct gcttgatggc gtgttcatcg aacttggtga actccccggc
     6301 aatacagatc agtcttgcgc catcccaatt aatatcttca ctggtgagtt tggggtctat
     6361 cttttgcgcg agtagcacga attcggctcg atattcgagc agccaatcca aataatacaa
     6421 gccttggttg ataacgttct cgttcaagga gcgcttatat tcgattatta cagggcaatc
     6481 gttctcgtca ataccgaggg tgtcaatgcg cccgccgtgt gttttgcctg tggagtgttc
     6541 cgaatcaaca agccgaatac caagaaatat ttcaaggttg ttttcaatga gggtttgtaa
     6601 agatttctca atggcaaccg aatgtccttt gataacttca acttgattac cttttgtatt
     6661 aaacaattta atatcgctca tgttttgacc tttatttatt atctgcgttt gaatatgact
     6721 gaagttcaac ccggtaattt attgggtttc cataatgatg atattcaagg gcgacatccc
     6781 ccacgcccgg acagacatac cttatggatg agtctccaga tgtgtaaaag acaattttat
     6841 agcaatctgt gaaactcccc gccggagtag ataggcttgt ttttatttcg acattatatt
     6901 tgtacatatg atcttcccct ggtgcggtga ttgctcccca actatttcct atttcaatag
     6961 ggaagatgat tttgggtgtg attttgtcag ttttatcggg tgactttatc tcttcataaa
     7021 gagattgaag gattgcaact tttccatctt ctgaacaagc cgtataaagt ttattttcga
     7081 aggtgatgta ccatttattt acaggccctt gtctggtttc ccatgtggtg caatcaaggt
     7141 cgtagatttc accagattgc tccactttat aaatatggat tttttcatat gcgccacttt
     7201 caacaaagat tactttttct gtgtacgtac ttatgtattc aattaatcct tccatcccat
     7261 cggttgttgg agtgctattt ccaactgtgt aggttcgggt acttcctaca aataatggaa
     7321 aatattctat taatggatca atattcgaat tttcgtctga ggtgctttct gttggttttt
     7381 ccagccagtg ttgaataact ggggtgagta cactggtaat taaagcaaaa aaaccagttc
     7441 ctactactgt tattatcgct gcctgaatag taacggatgt ctttttcttt gatggcattt
     7501 gatcagattc tacgaataca aatcccgggt agtgacagct agcttatact cgacaccgct
     7561 ttccttgaag acggcgaagt gtttggtgcc gcatttgatt ttcatttttt ctacttcgcg
     7621 caattcctgc cagttgatag ttcccttagt ctctgccaca aagtaaatac gtttgtcatt
     7681 ctcgaagact actgcccagt ctgggcggta attgccaatg ggggtaggga ctttgaagtc
     7741 gcgggggagt ttgaagaaga atttgatgct ttcgtcggct tcacagtctc gcgcaaagtc
     7801 cactttccct tcggaaaaga cttcggggaa caggtcacac aattgggcga gtttttcggc
     7861 tgtaaggtct aggctttttc cgtccatgta atttccttgt gtggttgttg cgaggattat
     7921 tctcgcaata ttatggttgt gttcttatat aaaagttatc aaaagccgct gggtcggtgg
     7981 ggtatcggga tgctacttca agtccaacca ttccatttgg gtaaggtgcg ccgctccatc
     8041 ggctaacttg attgccatct ataaacgtgg ttacgatgtc attttgcaat tgaatattaa
     8101 tcgagaaatc tgtagtcagg aaggacgaaa agccagcatt cgatatttct gtcaattcat
     8161 tgttcgatac gatataccaa ctccctctcg accaatctga atcgaacagg tcaaagtctc
     8221 gtatttcaag aagaagatag ttgctatcat caatggctcg gacgataata tgagcaatat
     8281 ttattcttgt ttcaatattg actaaatgta catcaactcc aacttcataa ttgccccgcg
     8341 tttgatcccc tgtcataatt ttgcctttgc tccaatcggt tccatcaagt acatttatga
     8401 ttgtgaggcg gttatttacc atttgccatt gcccagattt tatttgccaa ctgtcagaga
     8461 tgcctgtatt aaaatcatca attactaaat aggtgtatgt aggtgtggga gtggatggat
     8521 ttaacgtgtt cgttggaaat attgtaggta gaggaatagc agtatttgta ggggttggtc
     8581 tggcaagcaa ggaagcaact ccttttgtta caggtatcaa gaacgctgca cataagcata
     8641 atataaataa gccgcctagc aataatccaa acgcagagga acgaattgtt ctaattaacc
     8701 attctctagc ctctggagca ataaaaaata taatcgtgaa tatcacccca gtaatggtga
     8761 cgattgcctc aaatccttgc cagatgggat ctcttaaatc cataaaacca atcctagtta
     8821 ctagaatttt gagaaatgtt aatcaattcc ttacaggttg ggtagttcgg gcaagcccaa
     8881 aaatcctgtc ccttatgttc gcctcgttgg gcagtctttc tcaccattgg gattttgcag
     8941 ttcgggcaca aaggagcctc gcttggccct tccttttttt cttctttctc gaagaacggc
     9001 tggatttttt cttggatgaa ttctactttg aacgtcccgg tcacgggaat ccgcaccaaa
     9061 ggcagcatgg cagcagcgaa gaccctgttc ttgagttcat cccgccgctg tgcttctggc
     9121 ttgttgtggc tgctgtcgtc caactcgatc ccaaataaag gtctcaacgt tccaatttca
     9181 cagattaaaa agtcaatatg gcgggtggat atgcggtttc gcgctttccg gtattgttcc
     9241 ctgtcaagga tgtcgaatac atcctgcaac cccacctttg gcacgatcac tgcccggttt
     9301 tccagcgcct caagcagggc acgataaaac ctgctttctg cgggtgtcag aatgctgtcc
     9361 tttttgccat
//
LOCUS       DAOQJE010000017         9276 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_3944_length_9276_cov_6.464375, whole genome shotgun sequence.
ACCESSION   DAOQJE010000017 DAOQJE010000000
VERSION     DAOQJE010000017.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 9276)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9276)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9276
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3944_length_9276_cov_6.464375"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            189..1349
                     /locus_tag="PLT08_00845"
     CDS             189..1349
                     /locus_tag="PLT08_00845"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013646.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HNE03035.1"
                     /translation="MEKDILIIGGGPSGLSTALHLAKYAPHLTQRILILEKEHYPRLK
                     LCAGGLVIDAERILEHLGLDVTEIPHVDSNSIRFDFAGSGINIRVPKRHALRVIRRDE
                     FDHWLAKQVESKGIEIRQGVTVRKISPDANGVTVETDQETLRATLVVGADGSNGITRR
                     SIFPNEPIYTARVLEVLTPVSPVSSNSTTPRNQTALFDFFPVPDNIAGYVWDFPTQIK
                     GMEMRCWGIYDTNLLASQKRPALKDPLAKEMQRHGFDLNEYEIKGHPIRWYSPGNKAS
                     VSRVLLVGDAVGADVIFGEGISMALGYGNLAAKEIADSFQRGDFSFRRYKHRLERSGL
                     GQTLFARWLIAQIIYNLKWRWFQILLWRIVKPIVLFVAWVFVLNWSQRDLRY"
     gene            1379..2149
                     /locus_tag="PLT08_00850"
     CDS             1379..2149
                     /locus_tag="PLT08_00850"
                     /inference="COORDINATES: protein motif:HMM:NF014826.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HNE03036.1"
                     /translation="MGTADLHVHSIYSRDATTTVRAILKQASSVGLDIIAVTDHDEIR
                     GSLEAQQLAPQYGIEVISGAEVSTKDGHLVALFIKDLPPAGMSLTDTLLHIGDQGGLA
                     IIPHPFNNLPNSLTLEAVFTAMENPTAKASLKGMETHNFGTQNFDKMARKLSVFLPFS
                     KISSSDAHIYWAIGAGRTEFPGKTTQDLRDAIENNKTVPLLYEGESSAKALLSWARRI
                     TMRRFGYASDTSSATEDINTRQMSDSLIRRIQKKESSS"
     gene            complement(2160..2918)
                     /gene="recO"
                     /locus_tag="PLT08_00855"
     CDS             complement(2160..2918)
                     /gene="recO"
                     /locus_tag="PLT08_00855"
                     /inference="COORDINATES: protein motif:HMM:TIGR00613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HNE03037.1"
                     /translation="MTDFRSFRASAVVLRHADWGEADRILTLYTRDQGIVRALAKGAR
                     KVTSRKAGHLQPFTHVTVQLAKGRDLLIITQVETINAYLPLHDDLIKTGYAAYIIELL
                     LRFSYEDEGANPAIFRLVTETLDRLEKETDAWLPVRYYEMRLLDAVGFRPQLFQCANC
                     SREILPEDQYFSFTAGGVICPRCGEGIPNLAHISLEMLKYLRHFQRSSYKEASKARPS
                     LEVQKEAEVLMQGYFTYLLERELNTPGFLKKVKT"
     gene            complement(2961..3371)
                     /gene="cdd"
                     /locus_tag="PLT08_00860"
     CDS             complement(2961..3371)
                     /gene="cdd"
                     /locus_tag="PLT08_00860"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004126 - cytidine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0008655 - pyrimidine-containing compound
                     salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HNE03038.1"
                     /translation="MTISTVEKQSLIDLANEARRRAYVPYSNYPVGAALRTKTGRIYT
                     GVNVENAAYPQTMCAERVAIFKAVSEGETEFEVIAVVTNNGGSPCGGCRQVMAEFGLD
                     AIVLFGNGQGELTHETTVHDLLPEAFTPKHLVTE"
     gene            complement(3384..4748)
                     /gene="glmU"
                     /locus_tag="PLT08_00865"
     CDS             complement(3384..4748)
                     /gene="glmU"
                     /locus_tag="PLT08_00865"
                     /EC_number="2.3.1.157"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003977 - UDP-N-acetylglucosamine
                     diphosphorylase activity [Evidence IEA];
                     GO_function: GO:0019134 - glucosamine-1-phosphate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HNE03039.1"
                     /translation="MKITAVLLAAGQGTRMKSSLPKVLHPVAGKPMIWHALKAIQQST
                     TEKPVVVVGHGAEEVTNYLSDSAQTVLQEPQLGTGHAVMQAESLLRGKTDLVVVCYSD
                     MPLLRGETLQKLVETQKQNKGPVSLLTVVAENPRGFGRILRKDDGSVTAIVEEYVATP
                     EQLQVKELNVGGYCFDANWLWDALHRIPKNPKKGEYYLTDTIELAVKDGLSVHATVMD
                     DLEETIGVNTRVHLSEVESAMRRRINQTHMLNGVTMIDPSSAYIEAEVTIGKDTVIMP
                     NTYLFGNTEIGERNVIGPNTIIRNSKVGNGCKILASDLEGALLENDVDMGPFARLRKG
                     AHLGNHVHMGNFGEVKDSYLADGVKMGHFSYIGNAKIGSNTNIGAGTITANYDGEKKN
                     PTEIGEDVFIGSDTMLVAPVKLGDGARTGAGAIVTKNVPDDTLVVGMPARAIRKLERK
                     SKKK"
     gene            complement(4841..4914)
                     /locus_tag="PLT08_00870"
     tRNA            complement(4841..4914)
                     /locus_tag="PLT08_00870"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4880..4882),aa:Gln,seq:ttg)
     gene            complement(4944..6029)
                     /locus_tag="PLT08_00875"
     CDS             complement(4944..6029)
                     /locus_tag="PLT08_00875"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNE03040.1"
                     /translation="MIRNVLLVDDQRDILRLLRSTLDTLKNKDIQIYEAQSGEEAMLE
                     SSRHKIDLLVTDYKLPGMSGVELMHKVRARHAEVKVIFITGMTDRKMREDMLNSGAFG
                     IFDKPIPIADFLDSVERGLGFVQTIFPPELTAEANALGEGEIQQTKVSDLLANFRQDF
                     NANAVFLLNDRGLINARTGNLRDPSMEVSLISTLMAIHNTSLKVAKVNHQEVVESYHV
                     FSGGDHDLLFIPVTPLYALLVAGKDLATENQILDTVRGLMALRSQVEKALSTLGVTGQ
                     LKFKTAPLAVPPTEAPPKKNTDQLAKATPAPEMEALLSNASGEKAKEKSTDDFWESAA
                     QDLGRKSINPEVISLEEAKKMGLIPGE"
     gene            complement(6136..8061)
                     /locus_tag="PLT08_00880"
     CDS             complement(6136..8061)
                     /locus_tag="PLT08_00880"
                     /inference="COORDINATES: protein motif:HMM:NF012706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HNE03041.1"
                     /translation="MKKNVIPFLLVIVLIVIPACLSAPAQTETPAPTESFIEITETPP
                     PSLGPSLTVCLGQEPNSLYPFGDLNDAARNVLSAIYDGPIDNVGYEYQPVILTKLPSI
                     ENGDAQIVPIKINNGDSVVGADGNIIQFSKGSIVRPAGCRNADCAITYDGTSSIEMDQ
                     MIVTFRLRPDLTWSDGTPLTADDSIYSFELQKESKVNTYLTERTQTYEATDEQTLQWF
                     SIPGYLDPTYFLNFWQPAPKHAWSEFTPDQLPTVDVSSREPLGWGPYTVKEWLPGEGL
                     TLEKNPYYYRSRDGYPKLDSIQFRFIPDPDLALSELIAGRCDLIDPTINLEDKVGLLQ
                     EMQTSGQAKFFATTGMSMEWLGLGITPASYDNGYDIQKDRQNFFLDTYTRQAIAYCLD
                     RQTIATSVLHGLTEVPTSYLPLGHPALDTNLSAINYDPEIGNSLLDQAGWLDEDENPS
                     TPRRAINAQKVAYNTPLILNYQTTSTTQRRQVAAILEQSLANCGIGLNVEFISPNDLY
                     APGPVGPLFGRQFDLAEYALGVDGSEPPCGWFSTSSIPSEANSWSGTNVTGFSNEEYD
                     AACQAGQSALQEDESYLSAYRQTQIILADQLPAIPLFPRLHIAAARPDLCGFDLDPSA
                     RSLWNAEAFEISETCQN"
     gene            complement(8150..9139)
                     /locus_tag="PLT08_00885"
     CDS             complement(8150..9139)
                     /locus_tag="PLT08_00885"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017953348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter ATP-binding protein"
                     /protein_id="HNE03042.1"
                     /translation="MNKINKQEQPELLVVDNLVKYFPVRTGLFQRVTAWVQAVDKVSF
                     AVKKGETLGMVGESGCGKTTVGRTLLRLIEPTSGNVTFEGKDVLKLNQRELKPLRRDM
                     QIIFQDPYASLNPRMPIGESVMEGLQIHNIGHPKERWEIAVNMLKKVGLEEYHARRYP
                     HEFSGGQRQRIGIARALALNPKFIVCDEPVSALDVSIQSQVLNILKDLQEEFGLTYLF
                     IAHNLSVVEHISDRVAVMYLGKMVELTDRDSLYREPLHPYTKALLSAIPVGHPNEKRE
                     RTILKGDVPSPLNPPKGCRFHTRCPIAVSQCSQEEPTFKEVRPGHWVACLLAQ"
BASE COUNT         2445 a         2249 c         2124 g         2458 t
ORIGIN      
        1 atgggtttgg tccggcattc attggaacca tcacactcta tacgatctcg gtccttatgt
       61 attgggcatt cttctggcgc cgcaccatcg accccgttcc agcctcggca gcagccgatt
      121 agaaaattct caccaattta gtaaaaacag agcattgaaa ttgctctgtt tttttataca
      181 atactctcat ggaaaaagat atcttgatca ttggcggtgg tccaagcgga ctctccaccg
      241 cccttcatct cgcgaaatat gccccgcatc tgactcagcg catcttgatc ctcgaaaagg
      301 aacattatcc acgcctgaag ctttgtgctg gcggcttggt catcgatgct gaacggattc
      361 tcgagcatct cggcttggac gttaccgaaa tcccgcatgt ggattcaaac tcgatccgct
      421 ttgatttcgc aggcagcggt attaacatcc gtgtaccgaa acgccacgcc ctgcgcgtga
      481 ttcgccgcga cgagttcgat cactggttgg caaagcaagt tgaaagcaag ggcatcgaga
      541 ttcgccaagg tgtgacggtt aggaaaatct ctccagacgc caatggcgtc accgttgaga
      601 ccgatcaaga gaccctacgt gctaccctcg tcgttggcgc agatggctcg aatggcatta
      661 cacgccgcag cattttccca aacgagccga tttatacagc ccgcgtactg gaagtactaa
      721 cgccggtttc gccggtttca agtaactcca caacacccag aaaccagaca gctttgtttg
      781 acttttttcc agtccctgat aatattgcgg ggtacgtttg ggattttcca acccaaatta
      841 aaggtatgga aatgcgttgc tggggcatct acgatacaaa tctgcttgca agccagaaac
      901 gcccggcact taaagacccg cttgcaaaag aaatgcagcg acatggtttt gatctgaatg
      961 aatatgaaat caaggggcac cccattcgtt ggtacagccc ggggaataag gcttcggttt
     1021 caagggtttt actggtagga gatgcagtag gtgccgatgt gatctttggc gaaggcatta
     1081 gtatggcatt agggtatggg aacctcgccg ccaaagagat cgcagattcg ttccagcgtg
     1141 gtgacttttc gtttcgcagg tacaagcatc ggctggaaag aagcgggttg ggtcaaacgt
     1201 tatttgcacg ttggctcatc gcgcaaatta tttataacct caaatggcgc tggttccaaa
     1261 tcctgctttg gcgcatcgtg aaaccaatcg tcctttttgt ggcatgggtt tttgtactaa
     1321 attggagtca aagagacctc cgatattgaa catataaaag aaaagaaagt aacagcacat
     1381 gggcaccgca gaccttcacg ttcactccat ctactcccga gacgcaacca ccactgtgcg
     1441 agccatcctc aagcaagctt caagtgtcgg gctcgacatt attgcagtga ccgatcacga
     1501 tgaaataagg ggctctctcg aagcacaaca actggcacca caatatggaa tcgaagttat
     1561 ttctggggct gaagttagca caaaggacgg gcacttggtg gcattgttca tcaaggacct
     1621 tccaccggct ggcatgtctc taaccgatac cttattgcat attggagatc agggtgggct
     1681 tgccatcatc cctcacccat tcaataatct tccaaacagc cttacgttgg aagccgtatt
     1741 cactgccatg gaaaacccca cagcaaaggc atcgcttaaa ggaatggaaa cccataactt
     1801 tggcacacag aactttgaca aaatggcgcg taaactttct gtgttccttc cattctcaaa
     1861 aatatcttca agcgatgccc atatctattg ggctattggc gccgggcgca cagagttccc
     1921 tggtaaaacc acgcaagatc tgcgtgatgc aatagagaat aacaaaactg tgccactgct
     1981 ctatgaaggc gaatcatctg ccaaggcgct tttgagttgg gcgcgccgca ttaccatgcg
     2041 caggtttggg tatgcttctg atacgagttc tgcgacagaa gatataaata caaggcaaat
     2101 gtcagattct cttatccgta gaattcaaaa aaaagagagc agttcgtaac tgctctcttt
     2161 tatgttttga ccttctttag gaagccgggg gtatttaatt ctcgttccaa caaataagtg
     2221 aaatagcctt gcataagaac ttccgcctct ttctggacct caagactggg tcttgctttt
     2281 gaggcttctt tgtagctcga acgttggaaa tgccgcaaat atttgagcat ttccaatgaa
     2341 atatgtgcca ggttcggaat cccctcccca caacgggggc agatcacccc tcccgccgta
     2401 aacgagaaat attgatcttc tggcaaaatt tcgcgcgaac aattagcgca ttggaataat
     2461 tgcgggcgaa accctacagc atccaataac ctcatctcat aatagcggac gggtaaccaa
     2521 gcatctgtct ctttttcaag gcgatccaaa gtttcagtca caagccggaa aatcgccggg
     2581 ttcgcgcctt catcttcata tgaaaaacgt aaaagcaatt caataatata tgcggcgtaa
     2641 ccagttttta tcaagtcatc atgcaggggt aagtaagcat taatcgtttc aacttgggta
     2701 ataatcagta agtctcgccc tttagcaagc tgaaccgtga catgggtaaa aggttgtaaa
     2761 tgcccggctt tccgtgatgt gacttttctg gcaccttttg ctaacgcacg cactatccct
     2821 tggtcacgag tgtaaagcgt gagaatacgg tcagcttcgc cccagtccgc atggcgaagc
     2881 acaactgcag aagcacgaaa cgaacgaaag tcggtcataa aagaacatgg agaatggttt
     2941 tgtccattct ccacaagttt ctattcagtc actaaatgtt ttggggtaaa ggcttcaggt
     3001 aaaaggtcat gaactgtagt ttcatgtgtc aactcacctt gcccgttgcc gaacaataca
     3061 atagcatcca agccgaactc tgccattact tgcctgcatc cgccgcaagg agaaccgccg
     3121 ttattggtaa caactgcgat cacttcaaat tctgtctcac cttctgaaac ggctttgaaa
     3181 atcgccacgc gctccgcaca catggtctgt ggataggcag cattctccac attcacaccg
     3241 gtgtaaatgc gcccagtctt ggtccgcaag gctgcgccta cggggtaatt cgagtaagga
     3301 acatatgcac gacggcgtgc ttcatttgca agatcgatca aagattgttt ttctacagtt
     3361 gagatcgtca ttctaaacct gttctatttc ttttttgatt tccgttccag tttacgaatc
     3421 gcacgagctg gcatccccac taccagggta tcgtctggaa cattcttcgt tacaatcgcg
     3481 cccgcgcctg tgcgcgcgcc atccccaagt ttaaccggcg ccaccaacat ggtatctgac
     3541 ccgataaata catcttcacc gatctctgtt gggtttttct tctcgccatc gtaattggca
     3601 gtgatcgtcc ctgcgccgat gttggtattc gaaccgatct tcgcattccc aatatacgag
     3661 aagtgaccca ttttcacacc atcagcaaga taagaatctt tcacttcgcc aaaattcccc
     3721 atatgaacgt gattccccaa atgtgcacct ttgcgtaaac gggcaaaggg tcccatatcc
     3781 acatcatttt caagaagggc accttcaagg tctgaagcta aaattttgca gccattccca
     3841 actttagaat tgcgaattat cgtgtttggt ccaatcacat tccgttcgcc aatttctgtg
     3901 tttccaaaca aataggtatt cggcataatc actgtatctt tgccaatcgt cacttcagct
     3961 tcgatataag cagatgatgg gtcaatcatt gtcacaccat ttaacatatg cgtctgattg
     4021 atacgtctac gcatggcact ttccacttca gaaagatgca cacgagtatt cacaccaatc
     4081 gtctcttcga ggtcatccat cactgtagca tgaacagaca aaccatcttt gactgcaagt
     4141 tcaatggtat ccgttagata atattcgccc tttttgggat tcttagggat acgatgtaaa
     4201 gcatcccaca gccaatttgc atcaaaacaa taaccaccca cgttcaattc tttgacctgt
     4261 aactgctcag gtgttgctac atattcttcc acgatggcag tcaccgatcc atcatcctta
     4321 cgaaggatac gaccaaatcc gcgtgggttt tctgcaacta ctgttaataa agaaaccggt
     4381 cctttattct gcttctgcgt ttcaactagt ttttgtagcg tctctccgcg cagtaaaggc
     4441 atgtctgagt agcaaacaac tacgaggtcc gttttgccgc gcaagagaga ttcagcctgc
     4501 atcacagcat gccctgttcc tagctgcggt tcctgcagga ccgtttgcgc cgagtcgctg
     4561 agatagttgg ttacttcttc tgcgccatgc cccaccacca caacgggttt ctccgtggtc
     4621 gattgctgga ttgctttcaa ggcatgccag atcataggtt taccagctac agggtgcaac
     4681 actttcggca atgaggattt catgcgtgtg ccctgcccag cggcaagaag aacagcggtt
     4741 attttcatgg attcacctca gataaaaaca ggatttgtgc ggttgacgtg ctccgcgaca
     4801 aatcccgttt gagaaaattg tccgcttgcg cggacatgtc tggctggggc acctggattc
     4861 gaaccaagat ccacagctcc aaaggctggt gtgctgccat tgcaccatgc cccagtgcgg
     4921 gcaagattgt atcacaattt tttttattcg ccgggtataa gtcccatttt tttagcttct
     4981 tccagagaga tcacttcagg gttaatagac tttctaccca agtcttgcgc cgcagactcc
     5041 caaaaatcat ctgtcgactt ttccttggct ttctcgccag aagcattact cagcaacgcc
     5101 tccatttctg gagcgggggt tgctttggct aactggtcag tattcttctt tggaggtgct
     5161 tctgtaggcg gaactgctag tggtgcagtt ttaaacttta attgtccggt aacccctaat
     5221 gtggataatg ctttctctac ctgagaacgc aaagccatca agccacgcac ggtatccaga
     5281 atttgatttt cagttgccaa gtcctttcca gcgacaagca acgcataaag cggagtcact
     5341 gggataaaca gcaaatcatg gtctccccca ctaaagacat gataggactc aaccacttct
     5401 tgatgattaa cctttgccac tttcaaactg gtattatgaa tcgccatcaa agtggaaatc
     5461 aatgagactt ccatactggg gtcacgaagg ttcccggtgc gggcattaat caaaccgcga
     5521 tcgttaagca gaaatacagc attggcattg aaatcctgcc tgaaattggc aagcaagtca
     5581 gaaactttcg tctgttgtat ttccccttcg ccaagtgcat tcgcttcagc ggtaagctcc
     5641 ggtggaaaga tggtttgtac aaaaccaagt ccacgttcaa cagagtctag aaaatcggca
     5701 attgggatag gtttatcaaa gatgccaaaa gcgccgctgt tgagcatatc ttcgcgcatt
     5761 ttgcggtcgg tcatgccggt aatgaaaata acttttactt ctgcatgacg agcacgaact
     5821 ttatgcatta actccacgcc actcatgccg ggaagtttgt aatcagtcac taaaagatct
     5881 attttatgac gggaagactc caacattgcc tcttcccctg attgggcttc gtagatctga
     5941 atatccttat tcttgagcgt gtctaaggtg gaacgcaaaa gtcgtagaat atcgcgttgg
     6001 tcgtccacta acaaaacgtt tcgaatcata ccagcctgtt gtttgaaatc aatggaataa
     6061 attcgtttgg attaataaca tccaaacttg ataacgatgt ctaaattata gcaagattca
     6121 ggaattgctt cccaatcagt tttgacaggt ttcgctgatc tcaaatgcct cggcattcca
     6181 aagcgaccgg gcagatgggt caaggtcgaa accgcaaagg tcggggcgag cagccgctat
     6241 gtgcaagcga gggaaaagcg ggatggctgg caactgatcc gccaaaatga tctgagtctg
     6301 gcgatatgct gatagataag attcatcttc ctgtaaagcg gattgccccg cctgacaggc
     6361 ggcatcgtat tcctcattac taaagcctgt tacatttgta ccagaccatg agttcgcctc
     6421 acttggaatt gatgacgtac tgaaccaacc acatggtggt tcgctaccat ctacaccgag
     6481 agcatattct gctaggtcaa attggcgacc aaataagggt ccaaccggac cgggagcata
     6541 taggtcgttg ggcgatatga attcgacgtt caatccaatt ccacagtttg caagagattg
     6601 ctccaaaatg gcggctactt gcctgcgttg ggtagtggag gtggtttgat agttcaaaat
     6661 aagcggggtg ttataagcca ctttttgggc attgatcgcc cggcgtggcg tggaagggtt
     6721 ttcatcttca tccaaccagc ctgcttgatc taacaaagaa tttccaattt cggggtcata
     6781 gttaatggca gagagatttg tatccaacgc ggggtgaccc aacggtaaat aactggttgg
     6841 aacttccgtc agcccatgaa gaacggacgt ggctatagtt tggcggtcta aacagtaagc
     6901 aatggcttgg cgggtatatg tatccaagaa aaagttttgg cggtctttct gaatatcata
     6961 gccgttatca taagaagcag gtgtaattcc caagccaagc cattccatgc tcatgccagt
     7021 ggttgcaaaa aactttgcct gaccagaagt ctgcatttct tgcagcaatc caactttgtc
     7081 ttcaaggtta atggtagggt cgatcaagtc acagcgacct gcaattaatt cggacagagc
     7141 cagatctggg tcgggaatga agcggaattg aatgctatcc aatttaggat acccatcgcg
     7201 agaccggtaa tagtaagggt tcttttcgag cgtgagtcct tcgcctggca accattcttt
     7261 gacggtgtaa ggaccccatc ctaatggctc acgtgaagag acatcaacag tgggcaattg
     7321 gtcaggcgtg aattcgctcc aagcatgttt cggagcaggc tgccaaaaat tcaaaaagta
     7381 ggtcgggtcg agataccccg gaatactgaa ccattgcagg gtttgctcat cggtggcttc
     7441 ataggtttga gtgcgctcag tgagatatgt attgactttg ctttcttttt gcagttcaaa
     7501 cgaatagatg gaatcatcag ctgtcagcgg agtgccgtca gaccaagtca ggtcggggcg
     7561 taggcgaaat gtcactatca tttggtccat ttcgatagaa gaggtaccat cgtatgtgat
     7621 ggcacagtca gcattacggc agccagcagg gcgcacaatg gagcctttac tgaactgaat
     7681 aatattccca tcggcaccaa caacagagtc cccattattt atctttatag gaacaatctg
     7741 cgcatcgcca ttttcaatag aaggaagttt ggtgagaatc actggctgat attcatagcc
     7801 cacattatca atcggtccgt catagatcgc agagaggaca ttacgagccg catcattcaa
     7861 atctccgaat ggatacaaac tattcggctc ttgccccaaa cagacggtaa gtgagggtcc
     7921 caaagaaggc gggggtgttt cagtaatttc tataaatgat tcagtcggtg ccggggtttc
     7981 tgtctgtgca ggcgcactta aacaggctgg aatcacaata agaacaataa caagtaaaaa
     8041 tgggatgacg ttttttttca tagctcctgc catatctgtt taaggcgttt cgtaattata
     8101 accactcccc tattataaaa agagaccgcg tgtgcggtct ctttattttt tattgggcta
     8161 acaaacaagc cacccaatga cctgggcgaa cttccttaaa ggttggctct tcctgcgaac
     8221 actgactgac tgcaattggg cagcgtgtat ggaagcgaca accctttggc gggttcagcg
     8281 ggctgggcac atctcctttg agaatagtgc gctcccgttt ctcattcggg tgccccacag
     8341 ggatggcaga aagcaatgcc ttggtgtaag gatgaagcgg ttcacgatac aacgagtcac
     8401 ggtcagtcaa ctcgaccatt ttgccaagat acatcaccgc tacacggtct gagatatgtt
     8461 ccaccacact aaggttatgg gcaatgaaaa ggtatgtgag accgaactct tcttgaaggt
     8521 cttttaagat gttaagcacc tgagattgaa tggaaacatc caaagcggaa accggctcat
     8581 cacaaacgat gaacttggga ttcaatgcca aagcacgggc gatgccgata cgttgacgct
     8641 gaccgcccga aaattcatgc ggatagcggc gggcatggta ttcttccaag cccacttttt
     8701 tgagcatatt gacagcgatc tcccagcgtt ctttcgggtg accaatgttg tgaatttgta
     8761 aaccttccat gaccgactcg ccgataggca tacgcggatt aagagatgca tatgggtctt
     8821 gaaagatgat ctgcatatca cggcgtaaag gttttagttc acgctgattt agttttaaga
     8881 catcttttcc ttcgaatgtc acattgccag aggtcggttc aatcaggcgc aaaagggtgc
     8941 gtcctacggt tgttttgcca caaccagact cgcccaccat gccgagggtt tcgccttttt
     9001 tcacggcaaa gctgactttg tccaccgctt gaacccaagc ggttacgcgc tgaaataaac
     9061 ctgtacgaac aggaaagtac ttgacgaggt tatcaaccac caacagttct ggctgttctt
     9121 gtttgtttat cttattcatt taaactgctt cctttattta ccagatttca gtggtgcagt
     9181 gtgaccatct gcatcttcgt acaaccagca gcgaacttta tgtccatctg ccacttgaat
     9241 caagtcagga tgcttttcag tacaaatgga gaggtt
//
LOCUS       DAOQJE010000018         8927 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_4175_length_8927_cov_7.009581, whole genome shotgun sequence.
ACCESSION   DAOQJE010000018 DAOQJE010000000
VERSION     DAOQJE010000018.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 8927)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8927)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8927
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4175_length_8927_cov_7.009581"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..798)
                     /locus_tag="PLT08_00890"
     CDS             complement(<1..798)
                     /locus_tag="PLT08_00890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HNE03043.1"
                     /translation="MSKIDWIQQEIDNLKSQGLYNNIRTIGSPQGAWLTVDGKKVLNF
                     CSNNYLGLANHPALVAASKKATDEMGVGPAAVRSIAGTMSLHVELEKRLAKFKGVEAA
                     ITFQSGFTANLATVPALVGKEDVIFSDRLNHASIIDGCRLSGAKIIAYEHNDVKSLEE
                     QIKANLKDYRRALIITDGVFSMDGDIAPLPDIYEVAKKYDILLMVDDAHGEGVLGKGG
                     RGIVDHFGLHGKVDVEVGTMSKAFGVVGGIVAGKSVIIEWLRQRGRPF"
     gene            complement(883..1638)
                     /locus_tag="PLT08_00895"
     CDS             complement(883..1638)
                     /locus_tag="PLT08_00895"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HNE03044.1"
                     /translation="MTEKKLANIFIVLPAYNAAKTLHRTLDEIPADFRRNIILVDDAS
                     KDNTAELARGAGLTVFVHPKNRGYGGNQKTCYSEALASGADLVVMLHPDHQYDATVIP
                     HMVQPILDGEADAVFGSRMLGGRPLEGGMPWWKFNANVLLTAAANIIFRRYLTEIHSG
                     FRAYSRRYLETVRFMDNSDDFVFDTEIIAQGMINRLTFREVPIVTRYFAEASSINLQR
                     SIIYAIGILFVLLKYWLHNNGLVHFENFESKIK"
     gene            complement(1635..2312)
                     /locus_tag="PLT08_00900"
     CDS             complement(1635..2312)
                     /locus_tag="PLT08_00900"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNE03045.1"
                     /translation="MSNLSYQDKLSPWSSHSLIYRLIEKMPAHSKVLDVGTASGMLAR
                     REWARPLRFFGIEAVSEWAEIAAPFYEKIWVCAFDEASEEILGGYNAIVLADVLEHMP
                     SPDKALKKLVGLQSSGALFAVSVPNIANFWMRLNLLFGRFDYADRGIMDRTHLRFFTR
                     KTSRLLVENAGLDVMSIQVTPIPLELVSPFFLSRIGRMLHYIFAKLTSLFPTLLGYQI
                     IVEAKKP"
     gene            complement(2305..3468)
                     /locus_tag="PLT08_00905"
     CDS             complement(2305..3468)
                     /locus_tag="PLT08_00905"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021134.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 87 protein"
                     /protein_id="HNE03046.1"
                     /translation="MNKPFFFIFQPIRTPSFLKLFLFILVLLVCIVFIFIRPDNLDQS
                     DFMLIAWYPGQQLLHTGFVDANYPYPLWTVIVMLPLVVWAPKTAMMLMFVCNMLMLSA
                     SLTLFVLIFKWELSPVLLVLGVSLSVFFLPVLSSLWLGQLTIFSLLILALTLFFYFKE
                     KWGLLGAVLGLSFIKPQVMLLLVGLLLLWAFLKKKWEVLVGFSIVVTILILISLPLVS
                     TPMQLIGGGISSHLGSYILMTSTIWGATMSLGMSWLIPLLISITLLLWLGWIWLPFLV
                     GNKDRPHEFFLFSIAVIVNLVVIPYSWMHNLTLLLLPVGYSLLLISQKSNLVRVIGLI
                     LVFLVMYPLMYLLFILIGIPQVTQAYQIIPALFLLLGMMLLENKNIQTENFYV"
     gene            complement(3470..5068)
                     /locus_tag="PLT08_00910"
     CDS             complement(3470..5068)
                     /locus_tag="PLT08_00910"
                     /inference="COORDINATES: protein motif:HMM:NF025011.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-associated domain-containing protein"
                     /protein_id="HNE03047.1"
                     /translation="MPDLSTSLHKQDLGHFRIVAEFWGLELESTDSASALEELCGSLL
                     DLEAVSETLDILPAEARSALNELVSADGKIEWSIFSRKYGEIREMGAGKRDRERPYLK
                     PASTSEILFYRALIAKAFFESDKGSQEFAYIPEDLLEIINEVNVESAPLPSSAEALGR
                     PATPVEKAFEIPANDFVLDDATTYLVALRIGHESKANFKRELPLLLTTAGIIKNDGLL
                     PEAVKKFLEISRKEALDLLYNAWLESTTFDELRLMPSIVCEGEWKSQPQVTREFLMNL
                     INDVPEGKWWSIPAFVKAIKEKYPDYQRPAGDYDSWFIKRASDGQFMRGFAYWDSVDG
                     ALIKYLIQLLHWLGKADLAAPEEGKEPTAFRLVALHPESLRADVKINVVSNGRISVPR
                     TFSRTVRYQLARFCDWDEPKDDDYFYRVTAESLRRATEQGLKAEQLLAMLVKYTKEAV
                     PPAMVKALKRWDANGPEARVESLLVLRVSKPEIMEEMRKSKAAKYFGEVLSPTAIVVK
                     SGAIQKVLAELAELGLLAEISIPQ"
     gene            5179..5685
                     /locus_tag="PLT08_00915"
     CDS             5179..5685
                     /locus_tag="PLT08_00915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE03048.1"
                     /translation="MLILFSVLRFVHIIAGIFWAGGAILMNLIIGPAIGATGDAGKQF
                     VGHLMGKTIFSKFMLASGAITVLAGSILYGIDSNWFSSGWMMSPTGTGFGIGAISGII
                     ALVFGFLIGSTNGKLALLGMQIQGKPTNEQLAALGALRKRQVIVTNGNTIFILISIAL
                     MASARLFG"
     gene            complement(5731..6129)
                     /locus_tag="PLT08_00920"
     CDS             complement(5731..6129)
                     /locus_tag="PLT08_00920"
                     /inference="COORDINATES: protein motif:HMM:NF015444.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HNE03049.1"
                     /translation="MKTHTFRLKPQQDLFDSIEEFVKQNNIEAGCILSSVGSLTHATL
                     RLANREHYNEYEGHFEIVSMTGTVSIHGSHVHVAISDGDGVTIGAHLVSGCKVYTTAE
                     IVLLELDDVVYKRELFENDSGYEELVVYKK"
     gene            complement(6178..6738)
                     /locus_tag="PLT08_00925"
     CDS             complement(6178..6738)
                     /locus_tag="PLT08_00925"
                     /inference="COORDINATES: protein motif:HMM:NF025736.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HNE03050.1"
                     /translation="MSLRSILELDARLSNQMRVAEKPGILRNIAIFFAHSGDSWFWAA
                     ALIIIWFFSSSEWRHWETVEFFGLSGLAVVVLAVKFLVKRKRPEGEWGGIYRNTDPHS
                     FPSGHAARAFLIAVLASALAPTWLATILWVWAPLVALARVAMGVHYLSDIIAGAILGI
                     IVALVGLQFYQYLVDWFAGLTGFHFW"
     gene            complement(6735..>7549)
                     /locus_tag="PLT08_00930"
     CDS             complement(6735..>7549)
                     /locus_tag="PLT08_00930"
                     /inference="COORDINATES: protein motif:HMM:NF024214.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HNE03051.1"
                     /translation="KMNAIVLAGGIPQPNDPLYAYSQGGSKALIDVAGKPMVQWIVDA
                     LAEAKSVDQIIVIGLSQKSELTCSKPLHYISNQGRMLANIVAGSEKSLELKPNIDHLL
                     VCSADIPALRGDMVDWLVKTAMQTDDELYYGVCPREVMEARYPNSKRTYTKLKEMELC
                     GADVNLVRISIIKEHMAVWEALIGNRKSPLRQAGIIGFDTLFQLATRQFTLESLVERA
                     SERIGIKGRAIVWDKAEPCMDVDKPHQLELMREDLTRQIRSEKAKARTAEKK"
     assembly_gap    7550..7649
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(7709..8620)
                     /locus_tag="PLT08_00935"
     CDS             complement(7709..8620)
                     /locus_tag="PLT08_00935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE03052.1"
                     /translation="MKHAVSISIGSSKRNKAVEVNLLGETVRIERIGTDGDMEAAALK
                     YKELDGKVDAFGVGGADLEALVDGKVYPFYSVRPMVRYVEKTPLVDGGGLKNTLENKA
                     VAFLDAKIGDYINQHGGRKVLVPVGVDRWGLSKSFAESGYETVFGDMMFGLDIPIPVR
                     SLSALRTVAALLMPIAGRLPFEMLYPTGEKQEKHVPKFQKYYHWATVIAGDCHYVKRH
                     MPLDGMQGKVVVTNTTTPDDVEFFRKVGIKYLLTTTPVLEGRSFGTNMMEAALVAVSG
                     KNRPLSWDELGEMITKLGFEPQLQELN"
     gene            complement(8664..>8927)
                     /locus_tag="PLT08_00940"
     CDS             complement(8664..>8927)
                     /locus_tag="PLT08_00940"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNE03053.1"
                     /translation="KIEIGQRGRDGNMLLFFIKDNGIGIPHVHHERIFGLFNKLDAIS
                     EGTGIGLALVRRIIEVHKGRIWVQSEPGQGSTFFFTLPAGPES"
BASE COUNT         2583 a         2015 c         1906 g         2323 t
ORIGIN      
        1 gaagggacgt ccgcgctggc gcagccattc gatgatgacc gatttgcccg cgacgattcc
       61 acctaccacg ccgaacgctt tggacattgt gcctacttcc acgtccactt tgccgtgcaa
      121 gccgaagtga tccacgatgc cgcgtccgcc cttgccgagc acaccttcgc cgtgagcgtc
      181 gtctaccatc aagagaatat cgtacttctt cgccacttca tagatatcag gcaggggcgc
      241 aatgtccccg tccatgctaa aaacgccgtc cgtgatgatg agtgcacggc gataatcttt
      301 caaattcgcc ttgatctgtt cttcgagcga tttcacatcg ttatgttcgt aagcaatgat
      361 cttcgcgcca gagagacggc atccatcaat gatggaggcg tggttgagtc tgtcagagaa
      421 gatgacatct tcttttccaa ccaaagccgg gacagttgct aaattggcag taaatccact
      481 ttggaaagta atcgccgctt ctacgccctt gaacttggcg aggcgttttt cgagttctac
      541 atgcaaagac attgttcctg caatcgatcg aacggcggcg ggaccgacac ccatttcatc
      601 ggtcgctttc tttgaagccg caaccaaggc gggatgattg gcgagcccca gataattgtt
      661 cgagcagaaa ttgagaacct tctttccatc cacggtcagc caggcgcctt gcggcgaacc
      721 gatggtacgg atgttgttat ataacccctg cgatttcaaa ttgtcgatct cttgttggat
      781 ccaatcaatt ttggacatat ttaatctcct tgtagaaatc ttttttcatt atagcgactt
      841 aataaaaaat ggacatcaca ttttcattgt gacatccatt ttttatttta tttttgattc
      901 aaaattttca aagtgaacca acccattatt gtggagccaa tacttaagca gaacaaacaa
      961 gatgcctatt gcataaataa tgctcctttg caaatttata gacgaagctt ctgcaaaata
     1021 tcgtgtaaca attggaactt cccgaaaagt cagtctatta atcatacctt gagctataat
     1081 ctcagtatca aaaacaaagt catcactatt atccataaaa cgaactgttt caaggtatct
     1141 tcgcgagtac gcgcgaaaac cagaatgaat ttctgtcaga tagcgacgaa aaataatatt
     1201 tgcagctgca gttaagagta cattagcgtt gaacttccac catggcatac cgccctcaag
     1261 agggcgtcca cctaacatac gtgaaccaaa gacagcatct gcttcgccgt caagaattgg
     1321 ctgaaccata tgaggaatta ctgttgcatc atattgatga tctgggtgta gcatcacgac
     1381 tagatctgca cctgaagcca acgcttcgct ataacaagtc ttctggttcc cgccatagcc
     1441 acgattcttg gggtgaacaa agacagtcaa ccctgctcca cgtgcaagtt cagctgtatt
     1501 atcttttgaa gcatcatcta caaggattat attcctacgg aaatctgccg gaatttcatc
     1561 taatgtacgg tgcaaagttt ttgctgcatt atatgcaggc agtacaatga aaatattagc
     1621 cagttttttt tcagtcatgg ttttttcgct tcaacaatga tttgataacc caatagtgtt
     1681 ggaaatagag aagttaattt ggcgaaaatg taatggagca ttctaccaat cctagataaa
     1741 aagaaaggag acaccaactc aaggggaata ggagtcactt ggatagacat tacatccaac
     1801 ccagcatttt ctactaacaa acggctggtt ttgcgagtaa agaaacgcaa atgtgtccga
     1861 tccataattc cacgatctgc atagtcgaaa cgtccaaaca acaaattaag tcgcatccaa
     1921 aaattggcaa tattaggaac tgaaacagca aacagagcac ctgaggattg caagccaact
     1981 aacttcttca aagctttgtc aggtgaaggc atatgttcga gcacatcggc taaaacaata
     2041 gcattgtatc cacccaatat ctcttctgaa gcttcatcaa aggcacacac ccaaattttt
     2101 tcataaaaag gagccgcaat ttccgcccat tctgatactg cctcaatacc aaaaaaacgt
     2161 agagggcgag cccactctcg acgtgcaagc attccagacg ccgtacccac atccaacact
     2221 ttgctatgtg caggcatttt ttcaattaac ctataaatca gtgaatggct tgaccatgga
     2281 cttagtttat cttgatagga cagattagac ataaaagttc tctgtttgta tatttttatt
     2341 ttccaacaac atcattccta ataaaagaaa caaagcggga ataatttgat aagcctgagt
     2401 tacttgcggt atgcctatca aaataaaaag taaatacata agcggataca ttaccaaaaa
     2461 aacaagtatc aacccaatca cacgcactaa attgcttttt tgggagataa gtaaaaggct
     2521 atagccaaca ggaagtaaca acaaagtaag attatgcatc caactgtaag ggataacaac
     2581 taggttaact ataacagcaa ttgaaaacaa gaaaaactca tggggtctat ccttatttcc
     2641 tacaagaaaa ggtagccata tccacccaag ccacaataaa agggttattg aaatgagtag
     2701 cggaataagc caagacatac ccaaactcat tgttgcaccc caaattgtac tagtcattaa
     2761 aatataactt ccaagatggc tacttatccc gccaccgatc aattgcattg gagtagagac
     2821 aagcggtagt gaaatcagaa tcaatatagt tacaacgata ctaaagccca ccaaaacctc
     2881 ccatttcttt tttagaaatg cccataacaa aagcaagcca acaagcaata gcataacttg
     2941 aggcttaata aaagatagac caagaacagc cccaagcaag ccccattttt ctttaaaata
     3001 aaaaaataga gtcaaagcaa gaataagcaa agagaaaata gttaattgcc ccaaccataa
     3061 actacttaat acaggcagaa aaaatacaga tagagaaaca cctagcacca gcaacactgg
     3121 acttaattcc cacttaaaaa tcaaaacaaa caaagtaaga ctagcagaaa gcattagcat
     3181 attgcagaca aacatcagca tcatagctgt ttttggcgcc caaactacaa gaggcagcat
     3241 gacaataaca gtccataatg gataagggta gttcgcatcc acaaagcctg tatggagtaa
     3301 ttgttgaccg ggataccatg cgattaacat aaaatcgctc tgatctaaat tatcaggacg
     3361 tataaaaata aaaactatac aaaccaagag aactaaaata aaaagaaaaa gctttagaaa
     3421 acttggtgta cgtataggtt gaaagataaa gaaaaaaggt ttattcatct tattgtggaa
     3481 tagatatttc tgccaacaaa cccaactcgg caagttctgc caacactttt tggatagctc
     3541 cgcttttgac cacaatggct gttggactta aaacttcgcc aaaatatttt gccgccttcg
     3601 acttacgcat ctcttccata atctcgggct tgctgactcg caacacaagc agactctcca
     3661 cccgggcttc aggtccattt gcatcccaac gcttgagcgc cttgaccatt gctggaggaa
     3721 ccgcttcttt tgtatactta accagcatcg ccaataattg ttccgctttc aacccttgtt
     3781 ctgtggcacg ccgtaatgac tctgctgtga cgcggtaaaa atagtcatcg tctttgggtt
     3841 catcccaatc gcaaaaacgc gccagttgat aacgaaccgt ccgtgagaag gtacgtggaa
     3901 cagaaatcct gccgtttgag acaacattga ttttcacatc tgctcttagg ctttcagggt
     3961 gtaaagcaac aagacgaaat gcggttggtt cttttccttc ttcaggcgca gctagatccg
     4021 ctttaccgag ccaatgcaat agttgaatca aatattttat taaggcacca tcaacagagt
     4081 cccaataagc aaacccgcgc atgaactgtc catcagatgc gcgtttgata aaccacgagt
     4141 cataatcacc agcagggcgc tgataatctg gatatttctc tttgatggct tttacaaaag
     4201 cgggaatact ccaccatttg ccctctggca catcgttgat gagattcatt aaaaattcac
     4261 gtgttacttg cggttggctt ttccactccc cttcacaaac aatactaggc atcaagcgca
     4321 attcatcaaa ggtcgtggat tcaagccagg cattgtatag aaggtctaat gcttctttcc
     4381 gtgagatttc aaggaatttc ttcacagctt ctggaagaag cccatcgttc ttgatgatgc
     4441 ctgcggttgt taataacaga ggcaattcac gtttgaaatt tgctttgctc tcatgcccaa
     4501 tgcgcaaagc aacaagatag gtagttgcat catccagaac aaaatcattt gcagggattt
     4561 caaaagcttt ttcaacagga gtagcagggc gacccaatgc ctctgctgaa gaaggtaatg
     4621 gcgcactttc tacatttact tcattgataa tctctaagag gtcttcaggg atgtaggcaa
     4681 attcttgtga gcctttatct gactcaaaaa aagctttggc tatcaatgca cgataaaaaa
     4741 gaatttcaga agtagatgca ggcttgagat aagggcgttc acggtctcgc ttgccggcgc
     4801 ccatttcgcg aatctctcca tatttgcgag aaaagatact ccattcaatc tttccatctg
     4861 cggatacaag ctcgttgagc gcagagcggg cttcggctgg gagaatatca agagtttcag
     4921 aaacagcttc aaggtccagt aaagagccgc agagttcttc caaggcagaa gctgaatcag
     4981 ttgattcaag ctccaagccc cagaactctg caacgatacg gaagtgtccc aaatcttgtt
     5041 tgtgtaagga ggtggacaaa tcgggcatgg ggttattcta catgaaggag caagacttta
     5101 gaaggatttt gttttgtgaa ttaaaatgga taaaatatat cttattatta gatttgataa
     5161 attttaaagg agagtaccat gttaattttg ttttctgttt tacgatttgt acatattatt
     5221 gcaggaattt tctgggcagg tggcgctatt ttgatgaact taattattgg acctgccatt
     5281 ggcgcaactg gagatgcggg caaacaattt gttggtcact taatgggaaa aactatattt
     5341 tcgaaattta tgcttgcttc gggagcaata accgttttgg ctggaagtat tctatacggt
     5401 attgattcaa attggtttag ctctggatgg atgatgagcc cgacaggcac tggcttcgga
     5461 atcggtgcta tttctgggat tatagcgtta gtctttgggt tcttaatcgg aagtacaaat
     5521 ggaaaattag ctttacttgg catgcagatt caaggtaaac ctacgaatga gcaattagcg
     5581 gctttaggtg cattgcgcaa acgccaggtt attgtaacaa acggaaacac aattttcatt
     5641 cttatatcga ttgctttgat ggcgtctgca cgattatttg gataaatgcg ttgcaataaa
     5701 agacagacac ccatcaaggg tgtctgtctt ttatttttta tacacgacca attcttcgta
     5761 gcccgagtca ttttcgaata attcgcgctt ataaactaca tcatctaatt cgagtaacac
     5821 aatctctgcg gtggtgtaga ccttacagcc gcttacgagg tgagcaccaa tggtaacgcc
     5881 atctccatca gagatagcga catgaacgtg tgaaccatga atcgatacgg tgccggtcat
     5941 ggagacaatt tcaaaatgcc cttcgtactc gttgtaatgt tcgcggtttg caaggcgcaa
     6001 agtagcatgg gttaggctac ctacactcga aaggatacag ccggcttcga tattgttttg
     6061 ttttacgaac tcttcaatgg agtcaaagag gtcttgttga ggtttaagac gaaaggtatg
     6121 tgttttcata ggttttataa aaagttcgtg cgagagttta gctctcgcac gaaaagatta
     6181 ccagaaatgg aagcctgtca gcccggcaaa ccagtcgact agatattggt agaactgcaa
     6241 gcccaccaag gcaacgatga tgccgagaat ggctccagca atgatatcgg agagataatg
     6301 cacgcccatg gctacgcgag ctaaagctac cagcggcgcc caaacccaaa ggatagtggc
     6361 aagccaggtc ggagcaagag ccgaggcaag gactgcgatg aggaaggcgc gggcggcgtg
     6421 tcctgatggg aaggagtgtg gatccgtatt gcggtaaatg cctccccatt cgccttcagg
     6481 gcgtttgcgt ttgacgagaa atttaaccgc tagcacgaca actgctaacc cggaaagacc
     6541 aaaaaattct acggtctccc agtgccgcca ttcggagctg ctaaagaacc agatgataat
     6601 gagggctgct gcccaaaacc atgagtcgcc ggagtgggca aagaagatgg cgatgttgcg
     6661 caaaatgccc ggcttttccg caacgcgcat ttgattggaa agtcgggcat cgagttcaag
     6721 gatagaccgc agactcattt tttctctgcc gtccttgctt tggctttttc agaacggatc
     6781 tgacgtgtaa ggtcttcgcg catcaattcc aactgatggg gtttatccac atccatgcag
     6841 ggttcagctt tatcccatac gatggctcgc cctttaatgc cgatgcgttc tgatgcacgt
     6901 tcgaccaaac tttcaagggt gaattggcgg gtagcaagtt gaaagagggt gtcaaagcca
     6961 ataatgccag cctgccgcaa gggactctta cggttgccga tgagggcttc ccaaactgcc
     7021 atgtgttctt tgatgatact gatacggacg aggttgacat ctgcgccaca aagttccatc
     7081 tcttttaatt tggtgtaggt gcgtttggag ttggggtagc gggcttccat cacttcacgc
     7141 ggacaaacac cgtagtagag ttcatcatcg gtttgcatgg cggttttgac gagccaatcg
     7201 accatgtcac cacgtaaggc agggatatct gcagagcaga cgagcagatg atcgatattt
     7261 ggcttaagct caagcgattt ttcagagcct gcaacaatgt tcgccaacat acgtccctga
     7321 ttcgagatat agtgcagtgg tttggaacag gtcaactcgc ttttttgcga aagaccgatg
     7381 acgatgatct gatctaccga tttggcttcg gcaagtgcat ccacgatcca ctgcaccatg
     7441 ggtttgcctg ccacatcaat cagtgccttg gagccgcctt gcgaatacgc gtagagcggg
     7501 tcattgggtt gagggatgcc tccagcgagg acaatggcat tcattttcan nnnnnnnnnn
     7561 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7621 nnnnnnnnnn nnnnnnnnnn nnnnnnnnna gcgggtcatt gggttgaggg atgcctccag
     7681 cgaggacaat ggcattcatt ttcatacgtt aattcaactc ctgcaattgc ggttcaaagc
     7741 cgagcttggt gatcatttcg ccaagttcgt cccaagaaag gggacggttc ttcccagaaa
     7801 cggctaccaa tgcggcttcc atcatattcg ttccgaacga acgcccttcc aatacaggtg
     7861 tggtggtaag gaggtattta atcccaacct tgcggaagaa ctctacatca tctggcgttg
     7921 tggtgttggt cacaacaact ttgccttgca taccatccaa tggcatatgg cgtttgacat
     7981 agtgacaatc gcctgcaatc acagttgccc agtgatagta tttttggaac tttggaacat
     8041 gtttttcttg tttttcaccg gttggatata acatctcaaa cggcaatcgc ccggcaatcg
     8101 gcattaacaa cgcggcgacg gttcgaagcg cgctgagaga gcgaacggga atgggaatgt
     8161 ccaaaccgaa catcatgtcg ccaaagaccg tttcatagcc agactctgca aaagacttag
     8221 ataagcccca acgatccaca cccaccggaa caaggacttt gcgtcctccg tgttgattga
     8281 tgtaatcacc aatcttggca tcgaggaaag caacggcttt attttcaagc gtgttcttaa
     8341 gcccgccacc atctacgagt ggcgtctttt ctacatagcg caccatcggg cgcactgaat
     8401 agaatgggta gactttccca tccacaagcg cttcaaggtc tgcgccacct acgccaaagg
     8461 cgtctacttt tccatctaat tctttatatt tgagagcggc ggcttccatg tcgccatcgg
     8521 tgcctatacg ttcgatgcgt actgtttccc ccagcaaatt tacttccaca gccttatttc
     8581 ttttggatga cccaatactg atactgactg catgtttcat gaagaaacct ccacgagatg
     8641 agatgcgcca gttatatcac gaatcaggat tcgggtccgg cgggaagcgt gaagaagaaa
     8701 gtggagcctt gtcccggctc actctggacc cagatcctgc ctttgtgaac ctcaatgatt
     8761 ctccgcacca gagctaaacc aatccccgtg ccttccgaaa tagcgtctag tttattgaac
     8821 aagccaaaga tccgttcatg atgaacgtgc ggaattccga tgccattatc tttaatgaag
     8881 aagagcagca tattcccgtc tctgccacgt tgaccaattt caatctt
//
LOCUS       DAOQJE010000019         8911 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_4185_length_8911_cov_5.776536, whole genome shotgun sequence.
ACCESSION   DAOQJE010000019 DAOQJE010000000
VERSION     DAOQJE010000019.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 8911)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8911)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8911
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4185_length_8911_cov_5.776536"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            230..853
                     /locus_tag="PLT08_00945"
     CDS             230..853
                     /locus_tag="PLT08_00945"
                     /inference="COORDINATES: protein motif:HMM:NF012304.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease H family protein"
                     /protein_id="HNE03054.1"
                     /translation="MARQKYYVVWKGRKTGIFTSWAECEKQVKGVVGAQFKAFESEAE
                     ADAAYLAKYEDYIGKVSTSGRWREASIKPLLPSVCVDAACSGAPGTLEYRGVNTETGE
                     EIFHSGPYADGTNNVGEFLAIVHALTWQEKHNIHIPIYSDSENAISWVHTGKCRTKLA
                     HTSKNSILFAIIKSAENWLAENELPEGKIIKWDTNVWGENPADFGRK"
     gene            919..2499
                     /locus_tag="PLT08_00950"
     CDS             919..2499
                     /locus_tag="PLT08_00950"
                     /inference="COORDINATES: protein motif:HMM:NF024790.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M50 family metallopeptidase"
                     /protein_id="HNE03055.1"
                     /translation="MRFFGLSWSLGRLRGVDIRFHFSMLFSIPIAYYLFDPIDLRGVV
                     EALLWLVGFTIFILLHEVGHAFAAQMVGVEVKSIVVWLLGGLTNLAYKPEKPVHNFFV
                     YAAGPLMNMLLAFLCVAAYILTAMLFLPFSKDPQTYIWIQTFQNLFFSLAIVNLILAL
                     FNLIPVYPLDGGNIFHAAMEWLFGKTNADRITLVVGIPFLLVLIVFGVISRDYVLLAF
                     CVLIALSISTLNHSLLRNINLGITYLFKRAGYYYLKGDYERAAQLYTAEIEKQPDDIN
                     NYLARAGCYLAMAQRERALADVDRVLKLHPNHFLALELRGEILMLNKDYDAAFAHFKQ
                     VQHINANWAVPYFDLASLYMERGELQPAIENFNKAVVLQPRMPLFYIIRSLAHFKAGD
                     LASAHHDQDVAVGLSPEEALVMVDVNLIVYEDHWDWAKDYYERILTKNPRNAFALQGF
                     AEACLVNQKWDEAVNFFSRAIEQNPREARLYVGRGKAYVELEKLQEAKVDFEKAVASS
                     EKLHLKRQAENFLRTLDI"
     gene            2514..3083
                     /locus_tag="PLT08_00955"
     CDS             2514..3083
                     /locus_tag="PLT08_00955"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013053.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase family protein"
                     /protein_id="HNE03056.1"
                     /translation="MKTALLVIDIQKDYFPGGKFPLVEPLAAAEKAYGLLQCFRENNE
                     KHVHIQHISLKPDATFFVRGTEGSDIHDAVAHFEGEPIVYKHYPNSFRETTLFDMLKG
                     WGIERVVITGMMTHMCVDATVRAAADFGFQVWVAEDACATRDLEHNGTVVPADHVHRA
                     FLAAFKSYGQVMMSEQVIARLAAEKASAR"
     gene            complement(3153..3971)
                     /locus_tag="PLT08_00960"
     CDS             complement(3153..3971)
                     /locus_tag="PLT08_00960"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024157.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNE03057.1"
                     /translation="MTAFPPNAYELIRPLLLNMDIHLIGRSILAKQTPAQIFVDNHEH
                     PKALFAQAGHRYILAGDPERDSFNLGIQKLFTNVIFPREIAAGHEGFALYYDSSTWEK
                     RIDTLLSGKKTIHADREYYVCKKLKHKWQDFLPEGFEIKTVDAALLADSNLKHLETLK
                     EEMTSERPSVEDFLAKSFGVCAIYQGELAGWCLSEYNANGRCEIGIETISDYRKRGLA
                     TALTLAFLEHAFSHGITEVGWHCFKRNEASAKTALKAGFEKVCDYQSFIVLLKE"
     gene            complement(4002..4610)
                     /locus_tag="PLT08_00965"
     CDS             complement(4002..4610)
                     /locus_tag="PLT08_00965"
                     /inference="COORDINATES: protein motif:HMM:NF015069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SemiSWEET family transporter"
                     /protein_id="HNE03058.1"
                     /translation="MNFISTLGFAGTIIDLLRAAPQLVRLLRERKSFGVSVDTSGTSC
                     VVSLGWTTYGIITNQIFVALASGAIASFFFIITVAALRLGRSAREFRVTPLWFIVLFA
                     AVTFFGKTGLGLMLSISALISNVPQIRVAYKEENLSGLSLGTWLLTLSGGLIWLIYGI
                     LQKDLTIMVSTIFQSVTSAIIIGLKLFKQRTENTTATRFISD"
     gene            4669..5796
                     /locus_tag="PLT08_00970"
     CDS             4669..5796
                     /locus_tag="PLT08_00970"
                     /inference="COORDINATES: protein motif:HMM:NF013594.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HNE03059.1"
                     /translation="MQTTLDPERQKQAKEYARIRRRLWLVDTLLNAAYAIVWLVFGWS
                     ISLREWLTTITTNEWLLVALYVAIFGGIFSVFTLPLGYYSGFILPHRFDQSNQSLKDW
                     VIDRIKGLAIGAPLGLLMLELLYLALRLTGSLWWLYAAGGLLLFNVILSNLAPVLIMP
                     LFNKYVPLGDEHKDLEERLLNLAKRANTKVKGVFKFDMSKRTKAANAALTGMGNTRRI
                     ILGDTLINEFTPDEIETVLAHELGHHVHKDIPFLIISGTLLTTIGLFIASQTLNYSVT
                     FFSFNSVADVAAFPALSLILGAYGLLTQPLENYLSRWRENMADDYALVSTGKHEAFAS
                     AFTRLANQNLGEIDPEKWVVFMFYSHPPLGERIAKARKFTA"
     gene            complement(5854..6714)
                     /locus_tag="PLT08_00975"
     CDS             complement(5854..6714)
                     /locus_tag="PLT08_00975"
                     /inference="COORDINATES: protein motif:HMM:NF014679.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005504 - fatty acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HNE03060.1"
                     /translation="MKIVTDCAADMSNEELKQLGIVQAPLFIQFPEGEVNSADISADE
                     FYNRLEAMRPQIPTTAQPSSGIFAELYRKLAQIDKNILSVHISSGLSGTINSARDGGE
                     QVKGEANVNFWDTLTLSGGERFQVMAAALADKAGWAMDKVQERLTKIREKTEVIYTLD
                     TLEYLARGGRIGRVQAMAGALLNLKPVIRVDTDGKYSTVAKGRSIGKSMSIIADHLVE
                     KYGNAPVWVTVLHGRFAEKADQLAEEFKQKLNVAKMEVQRISPVLGVHTGPGIVGAAV
                     VPMELMGDLV"
     gene            complement(6711..7343)
                     /locus_tag="PLT08_00980"
     CDS             complement(6711..7343)
                     /locus_tag="PLT08_00980"
                     /inference="COORDINATES: protein motif:HMM:NF025343.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HNE03061.1"
                     /translation="MQQRSEETRSKIIFSAVKLFSAKGYNTASVDDICADAGISKGAF
                     YHHFRTKQDLFLALLDGWLETIDNAIEASKDKTVPETFMQITEAFPYIFATANEGLPM
                     FLEFWLQANRDEKIWQASVKPYRRYHKYFTDLIKKGVDEGSFVEVDPDLASRMIISTA
                     MGLLLQSLMDPKGAKWEKVARDSTTMLVASMLKNKNTIQTTNQSNQKRKP"
     gene            complement(7515..8552)
                     /locus_tag="PLT08_00985"
     CDS             complement(7515..8552)
                     /locus_tag="PLT08_00985"
                     /inference="COORDINATES: protein motif:HMM:NF015715.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HNE03062.1"
                     /translation="MNLLEILQFALSKVWETFSGLWPLLLLSVLISVILKLYVDQDRV
                     AAFLKRNQSNSIAMSTALAIGTPFCSCGTTAVVLGMMASTIPWAPIVAFMVASPLTSP
                     QELFYSAGLFGWPFAITFFVASIILGLTGGAIAAFAESKGWLANQGRFASDKAPRADL
                     KPAPVSQTVFQPQALLQGQAQFATQTAGISLSLPVIASEPIPTKKVVTFKQFADETFT
                     TSWKLLAMFLGFAFLGYFLNGLIPQEWIKTAFGSGNAYSVPLAAILGIPFYLNTEASM
                     PLVKAFMDSGMSAGAALAFLITGAGTSIGALAGAMTIARWRVIGIVLGTLVTGAIVLG
                     MLYDLLAPLFL"
BASE COUNT         2237 a         1918 c         2259 g         2497 t
ORIGIN      
        1 aaaaaggaca gcgaaccatc ctgcgcggac atcatttcac gcgggagccg ttccactgcc
       61 ttgagagttg actgcttact tgggcggata ttgtctcagg cggtggttac gaaccccgcc
      121 acagacagga gcaaaattgc gaagtacttt tcattgagcg cgtattttac gtgattgatg
      181 tcgtttgtca agacaaatat tcaaaactga attgatgtaa aattgacgca tggcgcgtca
      241 gaaatattac gtcgtatgga aaggacgcaa gaccggcatc ttcacgtctt gggcggaatg
      301 tgaaaaacag gtgaagggcg tggtgggtgc gcaattcaaa gcgtttgaaa gcgaagccga
      361 agcagacgca gcctatttag ccaagtacga agactatatt gggaaggtat ctaccagtgg
      421 acgctggcgt gaggcaagca tcaaaccttt gttgccgtct gtttgtgtgg atgccgcatg
      481 cagtggggcg cccggcacgt tggaatatcg cggtgtaaat actgaaacag gcgaagagat
      541 ctttcattcg ggtccatatg ccgatgggac gaataatgtt ggggagtttt tggcgattgt
      601 ccatgcattg acctggcagg aaaaacataa tatccatatc ccaatctatt cagactcgga
      661 aaatgcaatt tcatgggttc ataccggtaa atgccgtaca aagttagcac atacctcaaa
      721 gaattctatt ttatttgcca tcatcaaaag tgcggagaac tggctggcag aaaatgaatt
      781 gccagaaggc aagatcatta agtgggatac caatgtatgg ggcgaaaacc cggcagattt
      841 tggtcgaaag tgaaaagtga gaatccattc aatggtgaga atcaatttca gttcccgtat
      901 gaggcggggg tttaagtcat gagatttttt ggtttatctt ggtcgctggg tcgtctgcgc
      961 ggagtggata tccgtttcca tttttcgatg ctgtttagca ttccgattgc ttattatctt
     1021 tttgacccga ttgacctgcg tggtgtggtt gaagctttat tgtggttggt tggctttacg
     1081 atatttattc ttttgcatga agtggggcat gcgtttgccg cgcaaatggt aggcgtggaa
     1141 gtgaaaagca ttgtggtctg gctcttgggc gggttgacaa accttgcgta taaaccagag
     1201 aagccagtac ataatttctt tgtttatgca gccggacctt tgatgaatat gctgttggcg
     1261 tttttgtgtg tggcggcata tattcttaca gccatgcttt ttttgccgtt ttcgaaagac
     1321 ccgcaaactt atatttggat acaaactttt cagaaccttt tcttttcgct tgcaattgtc
     1381 aatttgattt tggcattgtt caaccttatc ccggtttacc cgttggatgg cggtaatatt
     1441 tttcacgctg ccatggaatg gttatttggt aaaaccaatg cagaccgcat cactcttgta
     1501 gtgggtattc catttttgct ggtattgatt gtcttcggcg ttatctctcg tgattatgtc
     1561 ttgcttgctt tttgtgtgct gatcgcactt tcgatcagta ccttgaatca ttccttgctc
     1621 aggaatatca atcttggaat tacctatctc tttaagcgag ctggctatta ttatttgaag
     1681 ggtgattacg aacgtgccgc gcaactttat acagctgaga tcgaaaaaca gcctgacgat
     1741 attaataatt atttggcaag agctgggtgt tatcttgcca tggctcagcg tgagcgagca
     1801 cttgccgatg tagaccgtgt gctcaaatta catccgaatc attttctcgc acttgaattg
     1861 cgtggtgaga ttctgatgct caataaagat tacgatgctg cgtttgcaca tttcaagcag
     1921 gtgcaacaca taaatgcgaa ttgggcagtt ccttactttg accttgcttc actctatatg
     1981 gaacgtggtg agttacagcc tgcaattgag aactttaata aggctgttgt cttgcagccg
     2041 cgcatgcctt tgttctatat catccgttca ctggcgcatt ttaaggcggg cgacctcgcg
     2101 tctgcgcatc atgaccaaga tgtggcggtg ggactttcgc cagaagaagc cttagtgatg
     2161 gtggatgtca acttgattgt gtatgaagac cattgggatt gggcgaagga ttactatgag
     2221 cgtatcttga caaaaaatcc gcggaatgcc tttgcgcttc agggcttcgc cgaagcttgc
     2281 ctcgtcaacc aaaaatggga tgaggctgtg aactttttct cgcgtgcaat agagcagaat
     2341 ccacgagagg cacggcttta tgtggggcgg ggaaaagcat atgtagaatt agaaaaactc
     2401 caagaggcaa aagtagattt tgaaaaagct gttgcatcgt cggaaaaatt gcacttgaaa
     2461 agacaggcag aaaatttctt aagaaccttg gacatatagg aaaaggatgt gttatgaaaa
     2521 cagctttact tgtgattgat atacaaaaag attatttccc cggcgggaaa ttcccgctcg
     2581 tagaaccgct tgcggctgca gaaaaagcat atggactgct acaatgtttt cgcgaaaaca
     2641 acgaaaagca tgttcatatt caacatattt cgctcaagcc cgatgcgaca ttctttgtcc
     2701 gtggaacaga aggctcagat attcatgatg cggtggcgca ttttgaaggc gaacctatcg
     2761 tctacaaaca ttaccccaat tcgttccggg agacgacttt gttcgatatg cttaagggat
     2821 ggggaataga gcgtgtagtc atcacaggga tgatgactca tatgtgcgtg gatgcaactg
     2881 tccgtgcggc ggctgatttt gggtttcagg tttgggtggc agaagatgct tgtgccacgc
     2941 gcgatttgga acataatggc actgttgtcc ccgcggacca tgtccatagg gcgtttttgg
     3001 cggcgttcaa gtcgtatggg caggtgatga tgtcggagca ggtgattgcg agattggctg
     3061 cagagaaagc cagtgctagg taagtagagt caattatcgg ctaagaaaga agcgaagaag
     3121 ctgtcacgtt ttttgtgaca gcttctttta tcctattctt tgagtaagac gataaacgac
     3181 tggtaatcac agactttttc gaagccggct ttgagcgctg ttttcgcaga ggcttcgttg
     3241 cgcttgaaac aatgccagcc gacttcggtt atgccatgag agaaggcatg ttcgagaaaa
     3301 gcgagagtga gtgccgtagc gagcccgcgc ttgcgataat cactgatggt ttcgatgccg
     3361 atctcgcagc gaccattggc attgtattca gagagacacc aacctgccaa ttcgccttgg
     3421 tagatggcac ataccccaaa acttttagcg aggaaatctt ccacgctggg gcgttctgag
     3481 gtcatctctt ctttgagcgt ctcgaggtgt tttaggttgg aatctgcaag aagtgctgcg
     3541 tctacagttt ttatttcaaa cccttctggt agaaaatctt gccatttgtg ctttaacttt
     3601 ttgcagacat agtattcgcg atctgcatgg attgtctttt ttcctgaaag cagagtatca
     3661 attcttttct cccatgtaga tgaatcatag taaagcgcaa agccttcatg ccctgctgcg
     3721 atctcgcgtg gaaaaatgac gtttgtaaat aatttttgaa ttccgagatt gaagctatct
     3781 ctttcggggt caccggcgag aatgtatctg tgacctgctt gggcaaacaa agctttgggg
     3841 tgttcgtgat tgtccacaaa gatctgggct ggtgtttgtt tggcgagaat tgaacgaccg
     3901 atgaggtgaa tatccatgtt gagcagcagc gggcggatga gttcgtaggc gttgggaggg
     3961 aatgcggtca aagttgagtt tccaatcttt tgtcttttta gttaatctga aatgaaccgt
     4021 gtggcggtgg tattttctgt tcgttgtttg aagagcttca aaccaataat gatcgcgctg
     4081 gtaaccgact gaaagatagt ggaaaccata atcgtgaggt ctttttgcag aattccatag
     4141 atcagccaga ttaacccacc tgagagagtc agcaaccatg tgcccagcga aagcccagaa
     4201 aggttttctt ctttgtacgc cactcgtatt tgtgggacgt tcgaaatcaa tgcgctgatc
     4261 gaaagcatta gccccagacc ggtttttcca aaaaaagtta ctgccgcgaa gaggacaata
     4321 aaccaaagcg gggtcacgcg aaattcacgc gcgcttcgcc caaggcgcaa ggcagccact
     4381 gtgataataa aaaagaagct ggctatggcg ccagatgcaa gtgcgacgaa aatttgattt
     4441 gtgatgatgc cgtaagtagt ccacccgagg ctgacgacac agctcgtgcc agaagtgtcc
     4501 actgagacgc cgaaagattt ccgttcacgc aaaagccgta ccagttgtgg cgcggcgcga
     4561 agaaggtcga tgatggtgcc tgcaaatcca agagttgaga tgaaattcat ttttgtccta
     4621 atagggaagt tttactagcg atagtgagcg catattataa ttccgcctat gcaaacgacc
     4681 cttgaccccg aacgacaaaa acaagcaaaa gagtatgcac gtattcgccg ccgcctttgg
     4741 ctggttgata cactattgaa tgccgcctat gccattgtct ggcttgtatt cggttggagc
     4801 atttctctgc gcgagtggct cactacgatc acaaccaatg aatggttgtt agtcgcatta
     4861 tatgttgcca tctttggcgg catattctct gtctttactc tgccgcttgg ttattacagt
     4921 ggctttattc tcccacatcg ttttgaccaa tccaatcaat cgctcaaaga ttgggttatc
     4981 gaccgcatca agggacttgc cattggtgcg ccgcttgggt tgttgatgct cgaactactt
     5041 tatcttgcat tgcgcctcac tgggagtctc tggtggttat atgctgctgg tggattgcta
     5101 ctctttaatg tcattctttc caaccttgcg ccggtgctca tcatgccgtt gttcaataaa
     5161 tatgttccac ttggtgatga acataaagac cttgaagaac gtctgctcaa tcttgcaaaa
     5221 cgcgccaata ccaaagtaaa aggtgtcttc aagttcgata tgtccaaacg taccaaagct
     5281 gccaatgccg cattgaccgg tatgggcaat acgcgtcgta tcattcttgg cgataccctc
     5341 atcaacgaat ttacaccgga tgagatcgag acggttcttg cacacgagtt ggggcatcac
     5401 gtccataaag atattccctt cttgattatc tccggtacgc ttcttacgac cattggtctg
     5461 ttcattgcct ctcaaacctt aaactattct gtgacctttt tctcctttaa ttctgtcgca
     5521 gatgtggctg ctttccctgc actctctcta attcttggcg catatggctt gttgactcaa
     5581 cctctcgaaa attatctttc tcgctggcgt gaaaacatgg cagatgatta tgccttagtg
     5641 tccacgggca aacatgaagc ctttgcttca gccttcactc gtttggcaaa ccaaaacctt
     5701 ggcgagatcg accctgagaa atgggtggtc ttcatgttct actcccatcc gccattaggc
     5761 gagcggattg cgaaagctag aaagtttacg gcttaaaagt ataggtcacg cttaaagcgt
     5821 gacctattta tttcttgtct tccaatactt ttcttacacc agatctccca tcaattccat
     5881 cggcacaact gccgcaccga caatgccggg acctgtatgc acgcccaata caggcgagat
     5941 gcgctgtact tccattttgg cgacattcaa tttttgtttg aattcttcgg cgagttgatc
     6001 tgctttctca gcgaagcgac cgtgcaaaac cgtaacccaa actggagcat ttccatattt
     6061 ctcaacaaga tggtctgcga taatggacat ggatttgcca atggaacgtc cctttgcaac
     6121 ggtgctgtat ttgccgtctg tatccacacg gatgacaggc ttgagattga gtaaggctcc
     6181 agccatggct tgcacacgtc cgatgcgacc accacgagcg agatattcca atgtatcgag
     6241 agtgtagatc acttcggtct tctcgcggat tttggtcagg cgctcctgaa ctttgtccat
     6301 tgcccaaccc gctttatctg ccaatgccgc tgccatcact tgaaagcgct ctccaccaga
     6361 gagggtgagc gtatcccaaa agttgacgtt tgcttcgcct ttcacttgtt cgccaccatc
     6421 tcgtgctgag ttgatcgtgc cgctcagtcc ggaagagata tgcaccgata gaatattttt
     6481 atctatttgt gccagtttgc ggtacaactc cgcgaagatg ccgctggatg gctgtgctgt
     6541 agtcggaatt tgtgggcgca ttgcctccaa acgattgtag aactcatcgg cagaaatatc
     6601 tgctgagttt acttcgcctt cagggaattg gataaacagg ggggcttgta caatgcctaa
     6661 ttgtttcaat tcctcattgg acatatctgc tgcgcagtct gttacgattt tcatggtttc
     6721 ctcttttggt tagattgatt cgttgtttga atagtatttt tgtttttcaa catgctagcg
     6781 accagcatgg tggtgctgtc acgtgcgacc ttttcccatt ttgcaccttt ggggtccatg
     6841 aggctttgta gcaacaatcc cattgcggtt gaaatgatca tgcgtgaggc taagtcaggg
     6901 tccacttcga cgaatgagcc ttcgtccact ccttttttga tgaggtcagt gaagtatttg
     6961 tgatagcggc gataaggctt cacgcttgct tgccatatct tttcatcgcg gttggcttgt
     7021 aaccagaact caaggaacat gggcaagcct tcgtttgccg ttgcgaagat ataggggaat
     7081 gcttctgtga tctgcatgaa ggtctcaggg acagttttgt ccttcgaggc ttcgatggcg
     7141 ttatcgatgg tttccagcca gccatcaagc agggcaagga aaaggtcttg cttggtcctg
     7201 aagtgatgat agaacgcgcc tttgctaatg ccagcatccg cgcagatatc gtctacactg
     7261 gcggtgttgt agcctttggc tgaaaacagt ttgacggctg agaaaatgat cttcgaacgg
     7321 gtttcttcac tgcgttgttg catattcctt gtaactctct tgactctttc ggtgagggga
     7381 cggatagaaa taaaaaccga ctagtcggtt tgtttttacc tctgaaactt tgcactgtca
     7441 agatgaggga actcatgttt aaaagaaaag cggacagagc ccaaaggttc tgtccgctat
     7501 gttgagttga acgattataa gaataatggt gccagtaaat cgtacaacat gcccagaaca
     7561 atagcgcctg ttaccaaggt tccaaggacg atgccgatca cacgccaacg ggcgatggtc
     7621 attgcacctg ccaaggcgcc gatagacgtc cccgcgccgg tgatgaggaa ggcgagagcc
     7681 gcgcctgcac tcatgccaga gtccatgaag gctttgacga gtggcataga ggcttcggtg
     7741 ttgaggtaga agggaatgcc aaggatggca gccaaaggaa cgctatatgc gttgccagaa
     7801 ccaaacgctg tcttaatcca ttcttggggg atcaagccgt tgaggaaata cccaaggaag
     7861 gcgaagccaa ggaacatagc aagcagtttc catgaggtgg tgaaggtttc gtctgcaaac
     7921 tgtttgaagg ttacgacctt tttggttgga atgggttcgg atgcgatcac aggcaatgac
     7981 aatgaaatac cagctgtttg tgtcgcaaat tgtgcctgtc cttgtaagag ggcttggggc
     8041 tggaaaacgg tttgagaaac tggagccggc ttgaggtctg ctcgaggcgc tttatctgat
     8101 gcgaaacgtc cctggttggc gagccatcct tttgattcgg cgaaggctgc aatggcgccg
     8161 ccagtcaaac ccaagatgat ggatgcaacg aagaatgtga tagcgaaggg ccagccaaac
     8221 aaaccggcac tataaaaaag ttcttgcgga gaggtgagag gggaggcgac catgaatgca
     8281 acaatgggcg cccatggaat ggtggatgcc atcatgccaa gcacgacggc ggttgtaccg
     8341 catgagcaga aaggcgtgcc aattgctaac gctgtagaca tggcaatgct gttactttgg
     8401 ttgcgtttga gaaatgcagc gacacggtct tggtcaacat atagttttag aatgacgctg
     8461 atgagaacgc tcaagaggag caggggccat aagcctgaaa aagtttccca aactttgctg
     8521 agtgcgaatt gaaggatttc aagaagattc attgatcatc tccaaaataa agatgtgtgt
     8581 ttagaaggat attcatggtt ttatctttca tatagaagtt tatctataag taaaagcaaa
     8641 aaaatttacc aataatgtct ctctgcctgc caaatggcaa acagagatcg agcttagcag
     8701 caaccgctgg ggcagttgcc gccttcacaa cattcgcttt cgcaacaatc gttggtgacg
     8761 acaggtaatt cgttcatgag atactccttt cgtatagaag tttgtctatg gaactgattg
     8821 caaaaaacgg gatcatgtca cggccgtgga catatcccgg ttatgccttg agggttttca
     8881 acaggttttc agtagtaacg gattcgggcg c
//
LOCUS       DAOQJE010000020         8745 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_4297_length_8745_cov_6.099194, whole genome shotgun sequence.
ACCESSION   DAOQJE010000020 DAOQJE010000000
VERSION     DAOQJE010000020.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 8745)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8745)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8745
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4297_length_8745_cov_6.099194"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..269
                     /locus_tag="PLT08_00990"
     CDS             <1..269
                     /locus_tag="PLT08_00990"
                     /inference="COORDINATES: protein motif:HMM:NF012706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HNE03063.1"
                     /translation="GIQKVDTNTMLIHLSRQEPKLLNYLTQPAFAILSPSALQSPSYG
                     TRQSTIISSGPYVVTSWTEEKIKLSPNPQYWDTIAGNDISYILK"
     gene            complement(477..550)
                     /locus_tag="PLT08_00995"
     tRNA            complement(477..550)
                     /locus_tag="PLT08_00995"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(514..516),aa:Pro,seq:cgg)
     gene            complement(601..1527)
                     /locus_tag="PLT08_01000"
     CDS             complement(601..1527)
                     /locus_tag="PLT08_01000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE03064.1"
                     /translation="MRLRLFSIASLLGLLSACSFQSTPAPTPLPPDYLPTVVALTGQA
                     AFATAAALTPSATLPPTETLQPPVEQAIPPTATPTLAAGFTEYAQIRFISPGPMSSLT
                     SPFILQANIAAGESDKIQVDLLGEDGRVLYREIKKLTRNPLGVFQRFEISYEIRAVSE
                     AGYIRISTEDDYGRMQAVNTMPVLLYSVGTAQINPPGNFIYERIAMDGFKEKSNFYAG
                     QVALNGRIWPFSDQAFVVEMISTEGKVLSSRILGINGIDTQPFETILPYKVTEPTPVR
                     LTFRQDNPLIGISDPVLGKLAYLYTIEVILNP"
     gene            complement(1582..3078)
                     /locus_tag="PLT08_01005"
     CDS             complement(1582..3078)
                     /locus_tag="PLT08_01005"
                     /EC_number="3.4.17.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004181 - metallocarboxypeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase M32"
                     /protein_id="HNE03065.1"
                     /translation="MSEKLAKLKEILGEVSDLGHAGSVLSWDQQVNMPPGGAEARGQQ
                     LATIGKIAQEKFTSDEVGSLLEDLKKEFSDPETDEGAMIRVAARGYDKAKRVPSSFVA
                     EQAMVATKSLEAWVEAKSKSDFSIFRPHLEKNVELVKQYVSFFPPADHPYDTLLDDYE
                     PGMKTSEVREIFGNLRPKQVSLIKAVSEAKQVKDDFLHKSFNEKKVWDFSEKIIAKFG
                     YDFSRGRQDKAPHPFETTFSVNDVRITNRYEKNAPLATLFSAMHECGHALYELGVNPA
                     YERTALEGGTSLAVHESQSRMWENLVGRSMPFWEHFYPELKKTFPSQLDGVSVKNFYK
                     AINKVEPSFIRVNADEATYNLHIMLRLEIEIGMVDGSIAVKDLPEIWNEKMRDYLGVT
                     PPNDAQGVLQDIHWSYGSIGYFSTYALGNIISAQLWERINKDIKNLEDQIRKGQFAEL
                     REWLREKIHVYGHKYDPQDIVQKVTGSKIDSAAYVRYLTKKYSEVYGL"
     gene            complement(3245..3955)
                     /locus_tag="PLT08_01010"
     CDS             complement(3245..3955)
                     /locus_tag="PLT08_01010"
                     /inference="COORDINATES: protein motif:HMM:NF019844.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0070064 - proline-rich region binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HNE03066.1"
                     /translation="MFNSRTFFALLLFIFAVGCTPVTTQTVDSEPQVADATATDEVLL
                     PTATETPLPTATLVPATEEPSATPAPFVHVTAVNGNLYIRRGPSTAYDRIAVLKSATS
                     ATVIGQDMLSKWVQVQIPNSEQTGWVSLLTPYSRIDGDLQGIPAFTFTEWPEPAYIKN
                     CTEHDVIVLPNELYLYSLWTNANYLNYVQVDPGTYQIQDATLPDAPVYDTIDVREGET
                     FYITVNGSGDYHKCPENN"
     gene            complement(3957..5216)
                     /locus_tag="PLT08_01015"
     CDS             complement(3957..5216)
                     /locus_tag="PLT08_01015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNE03067.1"
                     /translation="MSFAQAIMEALESLNGNKMRSGLTVLGIVIGVAAVIAMLAVGNG
                     AQASITGSISSIGTNLLFIFGGAPSQDGPPGSGPRTSGNNERPLTLGDSNALLDTFAA
                     PSVEMVAPAIQGNALITFSSEKTSTSISGVTPEYSQVRNLEIAEGEFINQEHILGRMS
                     VVVLGPETAIDLFGHADGIVGETIRIEGQPFRIIGVLVARGGGAFGSEDNSAYIPFTT
                     AQARLIKRGARDEVDVIFVQATTADAVPSAVEEVSDIIRQRHRTPVGADDFTVFTQQD
                     FLSIFSQITGVLTIFLGGIAGISLLVGGIGIMNIMLVSVTERTKEIGLRKALGARKRD
                     ILLQFLTESSLLSLIGGIIGTIFGWVIARIVEQVATATGNTFVPIVGWDAILLSTSFS
                     AMIGLFFGIYPASRAANLEPVEALRYE"
     gene            complement(5213..5911)
                     /locus_tag="PLT08_01020"
     CDS             complement(5213..5911)
                     /locus_tag="PLT08_01020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNE03068.1"
                     /translation="MDWVIEARDLKKTYVMGEIEVQALRGVSFTVTRGEVVSIMGPSG
                     SGKSTMMNTLGCLDRPSSGEYILDGESVAQMNDDQLATVRNRKIGFVFQSFNLLSRLS
                     AVGNVELPLRYAGITQGRREKAQAALEAVGLKDRMFHRPYELSGGQQQRVAVARALVN
                     NPAIIMADEPTGNLDSKVGKEIMTLLLNLNKDLGTTLVIVTHDPKIAEQTQRVIRLKD
                     GELDSIDINSGGAQ"
     gene            complement(5913..7178)
                     /locus_tag="PLT08_01025"
     CDS             complement(5913..7178)
                     /locus_tag="PLT08_01025"
                     /inference="COORDINATES: protein motif:HMM:NF027890.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HNE03069.1"
                     /translation="MRQFFSRFSRRTWIIIAVVAVVLICVGVSIFRPKNEVAVFETVA
                     VERGDLAAYVGATGSVRARQTATLTWQTSGIVGEVNAVIGDRVERGFVLSGLDKATLS
                     QSIILAEADLVSAQKALDDLLQSNTSLLEAEKAVDKAEESYQKAYNWRMDLNGKVDIK
                     EFYYDQFGELKVKQYKGYASAETIADADRDLALAESKLEDARREYERLQKGPDSDEVA
                     AARARVAAAEATLNMAKIIAPFDGTITQSNPVVGDQVNPGTVGFRIDDLSSLLVDVQV
                     SEVDINNVAVGQDATLSFDAILGKSYNGKVVEVGQAGDTVQGVVSFTVTVELTDADEQ
                     VKPGMTTAVNIITEEVKNTLLIPNRAVRLVNGERVVYLEKDGQATPVTITLGASSDTM
                     SVLTSGDIKEGDLIILNPPSANGGPFGGG"
     gene            7500..>8745
                     /locus_tag="PLT08_01030"
     CDS             7500..>8745
                     /locus_tag="PLT08_01030"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HNE03070.1"
                     /translation="MQAVPSEFLAIDRKERANRPFFDLDLRAADDRKCDFGAVVIEFD
                     PERAMVEAARCIHCPDPAPCMVACPTANDIPSAMWLIEQGKFVEAAGIYHKTSSLPEV
                     CGRVCPHESLCQGSCVLNKHHTPVLCGELEAFTVDYERRTQGYLIPLTIPSGKKVAVI
                     GAGPAGLGCADILLQKGHEVTIFDAKPAPGGLLVYGIPNFKLAKDVWGEKWEEFERAG
                     AKFVPNTYIGKDKTIDQLFAEGFDAVFIGVGSEIDAKMEEAPGTDLPGVYEATDFLIR
                     GNIPEDQLPSDMKKPLEIGKRVVVVGGGDTASDCLRTAIRLGAEEVTCLYRRTEKEMP
                     GGKKDRKMARDEGAKYRFLTQPVKFIAGADGKLAAVECIEMKLGEPDAKGRRKPVPVE
                     GSNFSVAADTAILALGYWPDPVI"
BASE COUNT         2322 a         2057 c         2137 g         2229 t
ORIGIN      
        1 atggcattca aaaagtagat acaaacacca tgcttatcca cttaagtcgt caagagccca
       61 agcttcttaa ttatcttact caacctgctt ttgcgatttt aagtccaagt gcgcttcaat
      121 ctccatccta tgggacacga caaagtacaa ttatctccag tggtccgtat gttgtcactt
      181 catggacaga ggaaaaaatt aagcttagtc ctaacccaca gtattgggat actatagctg
      241 gtaatgatat cagctatatc cttaaataaa aagattttat gctaaattag ctgataatac
      301 aaaaataagt tatttgtact tatctagtgt aatcctatta ttgccatcac tgctatacaa
      361 gccatgatgg caataatatt taaagtaaaa agcaatactt caaacaaact tattcaactc
      421 tgaatgtaaa taatcaaaaa aaccgccttc gggttaggcg gtttacgtac atgctgtcgg
      481 ggtgcgggga tttgaacccc ggacctcacg gacccgaacc gtgcgctcta ccggtctgag
      541 ccacaccccg aaagaacagc gggcagatta taccattctg atttatttta gcaagtcaga
      601 ttaggggttg aggatgactt caattgtata aagataggca agttttccaa ggacggggtc
      661 actaataccg ataagcggat tgtcctgccg aaaggttaag cggactggtg taggctcagt
      721 cactttataa ggcaaaatgg tttcaaatgg ttgggtgtca ataccgttaa tacccagaat
      781 acgggaggat aacaccttcc cttctgtgga aatcatttca acaacaaaag cttgatcact
      841 aaagggccag atgcgtccat tcaatgctac ttgaccggca tagaagttcg atttttcttt
      901 gaacccatcc atggcgatac gttcataaat aaaattccct ggtgggttta tttgtgccgt
      961 accaacagag tagagcaaaa ctggcatagt attgacggct tgcattctgc cgtaatcgtc
     1021 ctcggtacta atgcggatgt agcccgcttc agacacggcg cgaatctcat aggagatctc
     1081 aaagcgttgg aaaactccca agggattgcg ggttaacttt ttgatctcgc gatacaacac
     1141 tcgcccatct tccccaagca aatccacctg gattttatcg ctctcacccg ccgcaatatt
     1201 ggcttgcaaa atgaaagggg aagttaggct ggacatgggt cccggcgaaa taaagcggat
     1261 ttgggcatat tcagtaaagc ctgctgcgag ggttggcgta gctgttggag ggattgcctg
     1321 ttctacaggt ggttgaagcg tttcagtagg cggaagtgtt gccgaaggtg taagagcagc
     1381 cgctgtagca aaggcagcct gtccggtgag tgcaaccacg gtgggtaaat aatcgggcgg
     1441 taaaggcgtg ggcgcaggcg tggactgaaa ggaacaagca gacaaaagtc cgagaagaga
     1501 ggcgatggaa aatagtcgaa gtcgcatagt ggataataga agagacgccc acaggcgggg
     1561 cgtctcttga gtcaaatcaa tttacagccc gtacacttca ctatatttct ttgtgagata
     1621 acggacgtat gccgccgagt cgatcttgga gccagtgact ttctgaacga tatcttgcgg
     1681 gtcatatttg tgaccataga catgaatctt ctcacgtagc cactcgcgca attcagcaaa
     1741 ttgacctttg cgaatttggt cttccagatt cttgatatct ttattgatac gttcccacaa
     1801 ctgagcagag ataatgttgc caagcgcata agttgagaag tatccgatgg aaccatacga
     1861 ccagtgaata tcttgcaaca caccttgagc atcgttcggc ggggtgacac caagataatc
     1921 gcgcattttt tcattccaaa tttcaggcaa gtctttcacg gcaatactgc catccaccat
     1981 gccgatctcg atttcaaggc gcaacatgat gtgcaggttg taagtggctt catcggcatt
     2041 gacgcggata aacgaaggct caaccttgtt gatggctttg tagaaattct ttacactaac
     2101 gccatccaac tgagatggga aggtcttctt cagttcggga taaaaatgtt cccaaaaagg
     2161 catggaacga ccaacaagat tctcccacat acgagattgt gactcatgca cagcgagcga
     2221 tgtgccgcct tccaaagcag tgcgctcata agcggggttc acacctagtt cgtataaggc
     2281 atgtccgcat tcgtgcattg cgctgaaaag agttgcgagc ggagcgtttt tctcgtagcg
     2341 attcgtgatg cgcacatcat tgacgctaaa cgtagtttca aacggatgtg gagctttgtc
     2401 ttgcctgccg cggctaaagt catatccgaa cttggcaata atcttttcgc tgaaatccca
     2461 cactttcttt tcgttaaatg atttgtgcag gaaatcatct ttaacttgtt tagcctcact
     2521 gacggcttta ataagcgaaa cctgcttggg acgtaaatta ccaaagatct cacgtacttc
     2581 agaagtcttc ataccgggtt cgtaatcatc caacaaagta tcgtacggat ggtctgccgg
     2641 cgggaagaac gaaacgtatt gcttcaccaa ttcaacattt ttttcaaggt gcgggcggaa
     2701 gatggaaaaa tcggacttac ttttggcttc gacccaagct tcaagagatt ttgtggcaac
     2761 catggcttgt tctgctacaa aagaagacgg cacacgtttg gctttgtcgt atccacgtgc
     2821 cgccacgcga atcatagcgc cttcatcggt ttcagggtcg ctgaattctt tcttcaagtc
     2881 ttcgagcaaa ctgcctactt cgtcagacgt gaatttttcc tgcgcgatct tgccaatggt
     2941 tgcaagctgc tgcccgcgag cttcagcacc accgggaggc atattcactt gttgatccca
     3001 actcaacaca gaaccagcat ggcctagatc agacacttcc ccaaggattt ctttcaactt
     3061 tgcgagtttt tcagacatca caattctcca ttgatatttg acgtcaaacg cttccaacca
     3121 gaccggggct tggtcttaca tccctgagca tttaccccgc tgtatgcggc gttttcgtca
     3181 tgattgtatc actttgccga ttaaggcaga gaacacggaa tgacctccgt gttctctgta
     3241 tatcctaatt attttcaggg catttatgat aatcgcccga accattgacc gtaatgtaaa
     3301 aggtctcgcc ttctcgcaca tcgatcgtat cgtatacggg agcatctggc agagtcgcat
     3361 cctgaatctg ataggtacca gggtccactt ggacatagtt caaataattg gcattcgtcc
     3421 aaagagaata caggtacagt tcgttaggca aaacaattac gtcatgctcg gtacagtttt
     3481 tgatgtaagc cggctcgggc cactcggtaa aggtaaaagc agggattccc tgtaagtcac
     3541 cgtcaattcg agaataaggc gtcagaagag aaacccagcc agtttgttca gaattaggaa
     3601 tctgaacttg cacccacttt gagagcatat cttgcccgat gacggttgca gaggtggcgc
     3661 ttttcagcac ggcaatacgg tcgtaggcgg tactgggtcc gcggcggatg tagaggttgc
     3721 cattaactgc ggtgacatgc acaaaaggtg cgggcgtggc agaaggctct tcggtagctg
     3781 gaacaagcgt cgccgtggga agaggcgttt ctgtcgcagt gggcaacaaa acctcatctg
     3841 tggcagttgc atccgcgacc tgcggttcac tatctacggt ctgcgttgtc acaggcgtac
     3901 agcctactgc aaagataaag agcagtaagg caaaaaacgt tcgagagtta aacatgttat
     3961 tcgtatcgca aagcttcgac aggttccaaa ttagcggcgc ggctggcagg gtaaatacca
     4021 aagaacagac cgatcatggc agagaaactt gtcgaaagca aaattgcgtc ccagccaaca
     4081 ataggcacaa aagtattgcc agtagcggtt gccacttgtt ctacaatgcg tgcaatgacc
     4141 caaccgaaga tggtgccaat aatgccgcca atgagactca acaatgatga ttcggtgagg
     4201 aactgcaaaa ggatgtcgcg cttgcgtgcg ccaagggctt tacgcaaacc aatctctttg
     4261 gttcgttcag tgaccgaaac cagcatgata ttcataatac cgataccgcc caccaaaagt
     4321 gagatacctg caataccacc aaggaagatg gtaagcacgc cggtgatctg gctaaagatg
     4381 cttagaaagt cttgctgagt aaagacggta aagtcatcgg caccaacggg tgtgcgatgg
     4441 cgctgccgaa taatatcgga gacttcttcc acggcagacg gaaccgcatc ggcagtggtt
     4501 gcctgaacaa agatgacatc cacttcatcg cgagcgccac gtttgatgag gcgagcttga
     4561 gcagtagtaa atggaatata ggcactgtta tcttcactac caaaagcacc gccgccacgc
     4621 gccaccaaaa cgccaataat gcggaaaggc tgaccttcaa tacgaatggt ttcacctaca
     4681 ataccatcgg catgaccgaa taggtcaatg gcggtttcag gtccaagtac aacaacggac
     4741 atgcgtccca aaatgtgttc ttgattgata aattcgcctt cggcaatctc caagttacgg
     4801 acttgtgaat attcaggagt aaccccagaa atagaggtgc ttgttttttc actagagaac
     4861 gtgatcaagg cattgccttg aatcgccggt gctaccattt ccacagaggg agccgcaaag
     4921 gtatctaaca atgcattaga gtcgcctaag gtcaatggac gctcattatt tccactggtg
     4981 cgtggacctg acccaggcgg tccatcttgg ctaggcgcgc caccgaaaat gaagagtaag
     5041 ttcgtaccaa tgctagaaat tgaaccggtg atggacgctt gtgcgccatt tccaacagct
     5101 aacatggcga tcactgctgc cacaccgatg acaattccca acacagtaag ccctgaacgc
     5161 attttgttgc cattcaggct ttcgagggct tccatgattg cttgtgcaaa actcattgtg
     5221 cgcctccgga gtttatatcg atggaatcta attcgccatc ttttaagcgg attacgcgct
     5281 gagtttgctc ggcaatcttc gggtcatgcg tgacgatgac aagggtggtc ccgagatctt
     5341 tattcaagtt aagcaaaaga gtcataatct ctttaccgac ttttgaatcc aagttaccag
     5401 tcggctcatc tgccatgatg atggcagggt tgttgacgag tgcgcgggca actgctacac
     5461 gctgttgctg accgcctgaa agttcatagg ggcggtggaa catacgatct ttaagaccca
     5521 cggcttcgag agcagcttgt gccttttcgc ggcgtccttg tgtgatgccg gcatagcgta
     5581 aaggcagttc tacatttccg acagcagaga ggcgcgaaag cagattaaag ctttgaaaga
     5641 caaagccaat tttgcgatta cgaaccgttg ccaattgatc gtcattcatc tgcgcaacag
     5701 actcgccgtc caaaatgtat tcacctgatg atgggcggtc taaacagcca agggtgttca
     5761 tcatggtcga tttaccggag ccggaaggac ccatgatgga aaccacttct ccgcgagtca
     5821 cagtaaatga aactccgcgt aaggcttgca cttcgatctc acccatgacg taggtctttt
     5881 ttaagtcgcg tgcttcaatg acccagtcca tgctagcctc caccgaaggg acctccattg
     5941 gctgagggcg ggttgaggat gatcaaatcg ccttctttaa tatcaccgct agtgagcaca
     6001 ctcattgtgt ctgaggaagc gcctaaggtg atggtgacgg gtgtcgcttg cccatctttt
     6061 tcaagataga ccacgcgttc gccattcact aaacggacag cgcggttcgg aatgagaagc
     6121 gtatttttca cttcttcagt gatgatgttt acagcggtgg tcataccggg cttgacttgt
     6181 tcatcagcat cggtcaattc aacagtgacc gtaaagctaa caacgccttg aaccgtatcg
     6241 ccagcctgac ctacttcaac gactttgcca ttataggatt ttccaagaat cgcatcaaac
     6301 gaaagggtgg catcttgccc aacagcaaca ttgttaatat ccacttcaga gacttgtaca
     6361 tcgaccaaca agctggagag gtcatcaatg cggaagccta cagtgccggg gtttacctga
     6421 tcacctacca ctggattaga ttgggtaatc gtgccatcaa aaggtgcgat gatctttgcc
     6481 atattcaatg tggcttcagc ggcagcaaca cgggcacgag ccgcggctac ttcatcagag
     6541 tccggtccct tttggaggcg ttcatattca cggcgagcat cttccagttt tgattcagcc
     6601 aaggcaaggt cgcggtcggc atctgcaatg gtttcagcac tggcatagcc cttgtattgc
     6661 ttgaccttca attcgccaaa ttggtcgtaa taaaattctt taatatctac tttgccattt
     6721 agatccatgc gccagttata ggctttttgg taggattctt cggctttatc aacagctttt
     6781 tcagcttcta gcaaagatgt gttcgattga agtaaatcat ctaaagcttt ttgcgcactg
     6841 accaggtcag cttcggcgag gataatgctc tggctgagag tcgctttgtc gagtccggac
     6901 aggacaaatc cacgttcgac gcggtctcca atcacagcat tcacttcccc tacaatgcca
     6961 ctcgtctgcc aggtaagggt ggcagtttgt cgggcacgaa cactgccagt ggcgcctaca
     7021 tacgctgcca agtcaccgcg ctctactgca acagtttcaa aaacggctac ttcgttcttc
     7081 gggcggaaaa tagaaacacc gacgcaaatt aggaccaccg ctacaaccgc gataatgatc
     7141 catgttcgtc gggaaaatct agaaaaaaat tgacgcattc gggagttcct ccaattaggt
     7201 tacagtaagc gtgcagtgct gttttcttac attgatatat cacactctac gcaaaagatt
     7261 gtggaaagtt tgtgcagatt ttaactaatc gattctatct ctttatcttg taatttgaca
     7321 gtcaaaaaag cgacatttca aaattacaag cgagcgatac ctattaatca aagactcata
     7381 aatttatgac gattgtcttt gggtttggtg acatttaaac ctagttctat ccgatttatt
     7441 tggttattat tcgggaaacc gctttatttg tatctttctc ccctttggag gcattctgca
     7501 tgcaggcagt acccagcgaa tttttggcaa ttgaccgcaa ggaacgggcg aaccgtccct
     7561 tttttgattt agatttgaga gccgcagacg atcggaagtg tgactttggg gctgtagtga
     7621 tcgaatttga cccggagcgc gccatggtgg aagcggctcg ctgcatccac tgcccagacc
     7681 cggctccgtg catggtggcg tgccccacag ccaatgatat tccctctgcc atgtggctaa
     7741 tcgagcaggg taagtttgta gaggcggctg gtatttatca caagaccagc tccctaccag
     7801 aagtttgtgg gcgcgtttgc ccgcacgagt cattatgcca agggtcttgc gtgctgaata
     7861 agcatcatac gcctgtactg tgcggtgagc ttgaagcctt tacggtagat tatgaacgcc
     7921 gtacacaggg gtatctcatt ccgttgacaa ttccttctgg caaaaaggtg gcagtgatcg
     7981 gggcaggacc tgccggtttg ggttgtgctg atattttgct acaaaaggga cacgaggtaa
     8041 caattttcga tgccaagcca gcccccggtg gcttgctggt gtatggcatt ccaaacttta
     8101 aacttgctaa agatgtatgg ggcgagaagt gggaagagtt tgaacgcgcc ggtgcaaagt
     8161 tcgtccccaa tacttatatt ggaaaagaca aaaccatcga tcagttattt gccgaaggtt
     8221 ttgacgcggt ctttattggg gtcggttctg aaattgacgc aaagatggaa gaagcgcccg
     8281 gcaccgacct gcccggagtg tatgaagcga cagattttct cattcgcggg aatattccag
     8341 aagatcaact tcccagtgat atgaagaagc cgctcgaaat cggtaaacgg gtggtggtgg
     8401 ttggtggcgg ggatacagct tcggactgtc tccgaaccgc gattcgtctt ggcgctgaag
     8461 aagtaacctg tctgtatcgc cgtactgaaa aagaaatgcc cggcgggaag aaagatcgca
     8521 aaatggcacg agatgaaggg gcaaagtatc gcttcctcac ccaacctgtg aagttcattg
     8581 ccggtgcgga tggaaaatta gccgcagtgg aatgcattga aatgaaactt ggcgagcccg
     8641 atgcaaaagg acgccgcaaa cctgtaccgg tagaaggttc gaactttagt gtcgcagcgg
     8701 ataccgcgat tctggcgctc ggctactggc cggacccggt gattg
//
LOCUS       DAOQJE010000021         8514 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_4467_length_8514_cov_5.479253, whole genome shotgun sequence.
ACCESSION   DAOQJE010000021 DAOQJE010000000
VERSION     DAOQJE010000021.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 8514)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8514)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8514
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4467_length_8514_cov_5.479253"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..199)
                     /locus_tag="PLT08_01035"
     CDS             complement(<1..199)
                     /locus_tag="PLT08_01035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNE03071.1"
                     /translation="MSTEHSIIETVDITKVYGMGDISVSALRGVSIQIQKGEFVAIMG
                     PSGSGKSTLMHILGCLSKPSSG"
     gene            complement(196..1674)
                     /locus_tag="PLT08_01040"
     CDS             complement(196..1674)
                     /locus_tag="PLT08_01040"
                     /inference="COORDINATES: protein motif:HMM:NF027890.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HNE03072.1"
                     /translation="MTTKLNETLGKLKNIPILSSLPNLPKKVIWVIIVSIVVLAGAGG
                     GYAYYQSTQTADESTSTSELQTAMATRGDLVIYASGTGTLVSANETELSFTSTGKVTE
                     VNVTVGEKVEAGTQLAKLDDTDAQATYAEANRAYLELTSPSAIATAQQSIADATSDVS
                     DAKNTLAYIISPEALQAEEKVEEAQTAIAEAEAKLKESPTDTDAKKALEDAQSDLEAS
                     QELLAQAQGRYTSYYLPKYFTDGKELNAPSEAEILAARADYEFAKATLQEAKWLYAAL
                     TGEEIPEDATGSGLTELEQACLNLESAKATLDGTQIIAPYAGTVMSIDISVGDSVSSG
                     SSAITLADLTQPYLEVYLDESDWSSVVVGYETDVIFDILPDKTFSGVVTQVDPGLYSS
                     NNTSVVRAFVKLTQIDEESFTLPLGTTASVDVIGGRAENAVLVPIEALHKAGDQYAVF
                     IMENDKPILHVVEVGIQDLLYAEIISGVKEGDLVTTGIVETQ"
     gene            2198..2719
                     /locus_tag="PLT08_01045"
     CDS             2198..2719
                     /locus_tag="PLT08_01045"
                     /inference="COORDINATES: protein motif:HMM:NF025716.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4405 domain-containing protein"
                     /protein_id="HNE03073.1"
                     /translation="MNASTKTNLVLDLTIFTAFLAIASPKLTGNTIHEWLAVAFGAAI
                     VTHLLFHWKWLVEVTTKFFKNFFSQSRLNYVVDLLFFLAMTATLFSGLMISKDVMSFF
                     GIQLNVSHSWESLHRLVSDVSVILLGIHFALHWKWIVTNVSRYIVSPVASLFKPQAQK
                     PVVSMALQTFKEK"
     gene            2722..3054
                     /locus_tag="PLT08_01050"
     CDS             2722..3054
                     /locus_tag="PLT08_01050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE03074.1"
                     /translation="MKTILRILVILLIAGIVSGGVYALVENTSLMSGAEAERGAPPQM
                     TGTDGQTFQPMERPDGGGDRDSASLSRGFGEVGVLLAKITGITVVIVLIQKALGLFQK
                     NKPVIARA"
     gene            3118..3537
                     /locus_tag="PLT08_01055"
     CDS             3118..3537
                     /locus_tag="PLT08_01055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE03075.1"
                     /translation="MKKTIPAFIAALLITALLGGSMLLMGQNAVGVSTAKAEAAQTTQ
                     TSADTVAQYEQVIAQYQARETQYQDLVSQASIQITTANQQIELANQQLQQYQSLLQQL
                     QSSGLITIASDGTVTVNQTSSSAFMQPPQGHHGEGNH"
     gene            3537..3815
                     /locus_tag="PLT08_01060"
     CDS             3537..3815
                     /locus_tag="PLT08_01060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE03076.1"
                     /translation="MNLYRNLLKIWIGIVSLAGFVTGWVFIARASELETVTYVGNTAI
                     TMPELQPIPTVDGLAGEQLTTSNVQTFTVNTVVQQQQTFTQPMRTGGS"
     gene            3815..4738
                     /locus_tag="PLT08_01065"
     CDS             3815..4738
                     /locus_tag="PLT08_01065"
                     /inference="COORDINATES: protein motif:HMM:NF014477.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HNE03077.1"
                     /translation="MQYYEFRAMNTTVTLGAESQYTTMAFQRAQDFIEKSEQRFSRFL
                     HNSELNALNRSAGMWFQASAEMMELITIALECHLATDRLFNPAILPDLQAAGYDRSMD
                     QLRLHGADVKPQRSPAPVLPFHNVQLDIDQNRILLPTNMQIDLGGIAKGWIAEKAATI
                     LAAYSSACVVNAGGDMFLIGHPNGQNWEVVLEDPLDQTQDVLMLLVEDGAVATSSTTK
                     RLWTQGEQVRHHLIDPRSGEPAKSPWLSVTVFAPHASQAETFAKALLIAGPEGAQALL
                     NKSPEISFIAVDTERQIWKSPTEREKLYEYA"
     gene            4725..5417
                     /locus_tag="PLT08_01070"
     CDS             4725..5417
                     /locus_tag="PLT08_01070"
                     /inference="COORDINATES: protein motif:HMM:NF013918.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferric reductase-like transmembrane
                     domain-containing protein"
                     /protein_id="HNE03078.1"
                     /translation="MSTHNQPQTENWLDIFTVVGVLVTSVFLLAIGLLALQTQTVKDF
                     FALDSVHIWWYVTRAAGLMGYLLFWLSTVWGFAISSKIFDSFLERMFTFDFHEHLSLL
                     SLGFLGLHVAVLLIEKVEPLSLTEVLVPFVSTYRPFWTGIGIITFYLTVLVTITFYIR
                     KWISMKTFRVIHYLSVAAYFGALLHSVYAGTDTSLTWVQWMYGGTTLVTVFFMVYWAA
                     LLWMRKEEKRPI"
     gene            5561..5794
                     /locus_tag="PLT08_01075"
     CDS             5561..5794
                     /locus_tag="PLT08_01075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE03079.1"
                     /translation="MPNQELLAHSFCPKCGGTLWIVDKSNSNAKFWARNPQEILCVNS
                     PKCDYRRKVTEDDRKTIAQNISCFEDSSLSFVY"
     gene            5940..6671
                     /locus_tag="PLT08_01080"
     CDS             5940..6671
                     /locus_tag="PLT08_01080"
                     /inference="COORDINATES: protein motif:HMM:NF027484.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NBR1-Ig-like domain-containing protein"
                     /protein_id="HNE03080.1"
                     /translation="MLAILLSLMINACDASDVNSIVDESAVNDIYTQVALTITAQAPI
                     ITVTPHATSTPFPTASGVSLGLKTSIPSPVSVSYNSGNSGAVGCDNSSYLSDATIPDG
                     TVLAPGTVFTKTWSLQNTGSCEWTTSYAMAFYSGNAMSGTTTALSETVSIGSSGDISV
                     ELTAPSTAGTYTGYWRLQNASGTSFGEAVYVQIVVSGSTSTPTATPTSTSEDDESTNT
                     PTATTAPTETSLPTETPVPTETVES"
     gene            complement(6693..8336)
                     /gene="pabB"
                     /locus_tag="PLT08_01085"
     CDS             complement(6693..8336)
                     /gene="pabB"
                     /locus_tag="PLT08_01085"
                     /EC_number="2.6.1.85"
                     /inference="COORDINATES: protein motif:HMM:TIGR00553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046820 - 4-amino-4-deoxychorismate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008153 - para-aminobenzoic acid
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate synthase component I"
                     /protein_id="HNE03081.1"
                     /translation="MEEVERLVNENGWTAAGFVSYEASPAFDRALRVISSKDGFPLLW
                     FGLYETSETIDSLEDYGTLLPLEWRPDIDREAYNNAIQSIKNQIAQGKTYQVNYTMRL
                     STDIKAYQQSEIDLQALQLFRHLTHSQNKYAAFLDIGNWAICSASHELFFDLDDDTIT
                     GRPMKGTIKRGRNTEEDTNAQNWLRASSKNRAENVMIVDMIRNDIGRIADLGSVHVPE
                     LFTIEKYPTLFQMTSTVKGKTKASVAEIFVNLFPCASITGAPKVSTMNIIADLETSSR
                     NIYCGSIGYMAPGRKARFNVAIRTALVDKQNRKAEYGIGGGIVWDSTSADEYSEALLK
                     AQVLTSPPQKEFSLFETVLWMPEEGYFLIDRHIARIIDSANYFGFKTENLKENFLKAL
                     NAISPKGNSSKRIKISLNSEGFITTEIKDFQPSAKVFQVCLAKEPVNSMDRFLFHKTT
                     NREVYEKAAITGFDDVLLYNEKDELTEFMIGNLVAKIDGMLFTPPVECGLLAGTFRAE
                     LVASGEVEERVLKIQDWAEFEAVFLINSLRKWVKVKISK"
BASE COUNT         2268 a         1969 c         1828 g         2449 t
ORIGIN      
        1 gaccagacga tggctttgaa agacagccaa ggatgtgcat cagcgtactt ttgccagagc
       61 ccgaaggacc cataatggcg acaaattcgc ctttttgaat ttggatgctc acgcctcgta
      121 aggcagagac gctaatatca cccatgccat aaactttggt aatatcgacg gtttcaataa
      181 tcgaatgttc tgtgctcatt gcgtctccac gatgccggtt gttaccaggt cgccttcttt
      241 tacgcctgaa ataatctccg cataaagcaa atcttgaata cctacttcaa ccacatgcaa
      301 aatgggtttg tcattttcca taataaaaac ggcatattgg tctcctgctt tgtgcaaggc
      361 ttcgatgggc accaacactg cattttcagc ccgcccgccg ataacatcga cagaagcggt
      421 tgtgcctagc ggtaaagtga agctttcttc atcaatttga gtcaacttta cgaaagcccg
      481 aactacagaa gtattgttgg aggaatacag cccagggtct acctgagtga caactccgct
      541 gaacgtttta tcaggcaaga tatcgaaaat aacatctgtc tcatagccca ccacaacgct
      601 cgaccaatct gattcatcta aatacacttc caaatatggc tgggttagat ccgcaagcgt
      661 aatggcagaa ctgccgctgc tcacactatc tccaaccgat atatcaatgg acataaccgt
      721 acctgcatac ggtgcaatga tctgtgtacc gtctaaagtc gcttttgcag attcgaggtt
      781 caagcatgct tgctcaagct cggtcaagcc gctgccggtt gcatcttcag gaatttcctc
      841 gccggtcaac gctgcataca gccatttcgc ttcttgtagg gtcgccttgg caaattcata
      901 gtctgcacgt gcggcaagaa tctcagcctc tgaaggtgca tttaattctt tgccatctgt
      961 gaaatatttg ggaaggtaat aacttgtgta acgtccttgc gcctgtgcca acagttcttg
     1021 ggatgcttca aggtcagact gagcgtcctc taaggctttt ttcgcgtctg tgtctgtggg
     1081 tgactctttg agcttcgcct cagcctcggc gatagctgtt tgtgcttctt caaccttttc
     1141 ctccgcctga agcgcttctg ggctaataat ataagctagg gtgttttttg catcactgac
     1201 atcactagtc gcatctgcaa tggattgttg agcagtagcg attgcgctgg gagaagttaa
     1261 ctctaaataa gctcggtttg cttccgcata tgttgcttgg gcgtctgtat catcgagctt
     1321 tgccagttga gtacctgctt ccaccttttc accaacggtc acattaactt cggtcacttt
     1381 gccggtagat gtaaaagaaa gctcggtttc atttgcagag actaaagtac ctgtgccact
     1441 ggcataaatg acaaggtcac ctctggttgc catagcggtt tgcaattcag aagtgctggt
     1501 gctttcatcc gcagtttggg tgctttgata ataagcatat cctccgccag cgcctgctaa
     1561 taccactatg ctgacgatga tgacccaaat cacctttttt ggtaggtttg gcaaagagga
     1621 aaggatggga atatttttta actttcctag tgtttcattt aattttgttg tcatgggctt
     1681 aatttccctg taaaaatgag atcagatgtt tctctttcaa tataaaaaac gaccctgtga
     1741 aaatactgtg aaaattgtga gcgtctcgtg catatttacg gacaaaacag aactagatga
     1801 gaaagattca catctagttc ttagcagggg tttatgaatt tttcggctaa ttctcaggat
     1861 gattttctat actgactttg ttcaaccaaa ccggttgaag aaaggagtgc ctagcgacct
     1921 tactcgcttc aatgtctcga tctctgaaag gaggtggatt ccctttagcg taaacgaagt
     1981 tttataagaa agttgagcgt ccctcgtagt gatacggggg acgctttttg ctttttatac
     2041 ccagtcttca caggaaattc acattcttta cagattttat acaaaccgtt cttggttttt
     2101 tcacagtttc gccttctaca atgcaggtgt tcgaataaat caaatagaaa catccccgcg
     2161 aaacaaaatt cgcggcaacg aaaggaaacc taaaaccatg aacgcatcga ctaaaaccaa
     2221 tctcgtactc gaccttacga tctttaccgc ctttttggcg attgccagcc caaagctgac
     2281 cggcaatacc atccatgaat ggctggcagt cgcatttgga gccgccattg tgacccactt
     2341 acttttccac tggaagtggt tggtggaagt gaccacgaaa ttcttcaaga attttttcag
     2401 ccagtctcgc ctcaattatg tagtggattt gctcttcttc ctcgccatga ccgccacgct
     2461 ctttagtgga ctgatgatct cgaaagatgt aatgtctttc ttcggaattc aactgaacgt
     2521 gagccatagc tgggaaagcc tgcaccgcct cgtttcagat gtatctgtga tcttactcgg
     2581 cattcacttt gccttgcact ggaagtggat tgttaccaat gtcagtcgct atattgttag
     2641 cccagtggcg agcttgttca agccgcaggc acagaaacct gttgtatcca tggcacttca
     2701 aaccttcaag gagaaataat catgaaaacc attttgagaa tccttgtcat cctgctgatt
     2761 gcaggaatcg tatccggcgg agtttacgcc ttggttgaaa acaccagcct catgtctggg
     2821 gctgaagcgg agcgcggagc gcctcctcaa atgaccggta cagatggtca gaccttccaa
     2881 ccgatggaac gacctgatgg cggcggagat cgtgactcag cctctttatc ccgcggcttt
     2941 ggtgaagtag gtgttttgtt agccaagatc actggcatca cagttgttat cgtgctcatt
     3001 caaaaagcac tgggcttgtt tcagaagaat aaacctgtta ttgcccgagc ctaaccctta
     3061 aaacagatat gagacatcgc aagtaagact tgcgatgtct catggagaga gaacctaatg
     3121 aaaaagacta ttcccgcatt tattgcagcc ttgttgatca ctgctttact tggtggcagc
     3181 atgctactga tggggcagaa cgcagtgggt gtttctaccg caaaagcaga agcagcacaa
     3241 acgactcaaa cttcggcaga tacggttgca caatacgaac aagtcattgc gcaataccag
     3301 gcaagagaaa cacaatacca agatttggtc agccaggcat caatacagat tacaacagcc
     3361 aatcagcaaa ttgaattggc gaatcaacag ttacagcagt atcaatcgct attacagcaa
     3421 ttgcaatcaa gcggcttgat cactatagcc agtgatggca ccgtgacggt caatcaaaca
     3481 agctcgtccg cttttatgca gccgcctcaa ggacatcacg gcgaaggaaa tcactaatga
     3541 acctatatcg caatctactc aaaatatgga ttggcattgt aagtttggca ggttttgtta
     3601 cgggctgggt ttttattgcc cgcgcctccg aacttgaaac tgtgacctat gttggcaata
     3661 ctgccattac catgcctgaa ctacaaccga tcccaactgt ggatgggttg gctggcgaac
     3721 agctaacaac gtctaatgtg caaaccttta ccgtcaatac ggttgtacaa cagcagcaaa
     3781 cctttactca acccatgcgt accggaggtt cataatgcaa tactatgaat ttcgcgccat
     3841 gaacacaacg gtaactttag gcgctgaaag ccaatacacc acaatggcgt ttcaacgcgc
     3901 acaagatttt attgaaaaga gcgaacaacg cttttcacgt tttctgcaca atagcgaatt
     3961 aaatgctctt aatcgtagtg ctggcatgtg gtttcaagct tcggctgaaa tgatggaact
     4021 gataactatc gcgttggagt gtcatcttgc gacagataga ttattcaacc cggcaatctt
     4081 gccagacctt caagcggctg gctacgaccg tagtatggat caacttcgtt tgcatggtgc
     4141 tgatgtgaaa cctcaaagat cacctgcacc cgttttgccc tttcataatg ttcagttgga
     4201 catagatcag aatcgtattc ttctcccaac gaatatgcag attgaccttg gtggtattgc
     4261 caaaggctgg attgctgaaa aagcagccac gatcttagca gcttattctt cagcttgtgt
     4321 ggtcaatgct ggcggagata tgttcctcat cggtcacccc aacgggcaga attgggaagt
     4381 ggtgctcgaa gacccgcttg accaaaccca agatgtcttg atgctcttgg tagaggatgg
     4441 ggctgtggcg acatcttcca caaccaaacg cctctggact cagggagagc aggttcgcca
     4501 ccatctaatc gatccacgct cgggcgaacc tgccaagtca ccttggctga gcgtcactgt
     4561 ctttgctcca catgcatcac aagcagagac ctttgctaaa gccctcttaa ttgctggacc
     4621 cgaaggcgca caggcgttat tgaataaaag cccagagatc tcatttattg ctgtagatac
     4681 cgaaagacaa atttggaaat ctcccactga aagagaaaaa ctttatgagt acgcataacc
     4741 aacctcaaac tgaaaactgg ctcgatatct ttaccgtggt gggcgtcctt gttacgagcg
     4801 tgtttctact agctataggt ttactcgcgt tgcagacgca aacggtcaaa gacttttttg
     4861 cgctcgatag tgttcacatc tggtggtacg ttacccgtgc cgcggggctg atgggatatc
     4921 tgttgttttg gctttccacc gtttggggtt ttgccatcag cagcaagatc ttcgactcgt
     4981 ttctggagcg catgttcaca ttcgattttc atgaacatct ctcattatta agtctggggt
     5041 ttttaggctt gcacgtggcg gttttgctca ttgaaaaagt agaaccctta agcctaactg
     5101 aagtcctcgt gccatttgtt tctacctatc gtccattttg gacgggaatt ggcatcatca
     5161 ccttctactt gacggtgctt gtgacaatca ccttttatat ccgtaaatgg atttcaatga
     5221 aaacttttcg agttattcat tatttgagtg ttgctgctta ttttggggca ctgctgcaca
     5281 gcgtctatgc tggcacagat accagcctta cctgggtgca atggatgtat ggcggcacaa
     5341 cacttgtaac ggtcttcttt atggtgtact gggcagcctt gctgtggatg cgaaaggaag
     5401 aaaaacgacc tatatagaat atatacaaat aaaaagccct gccagaacac tggtagggct
     5461 ttttattcac agatatttca caggcttttc atggcatttt ctttgcgttt tcaaagcaaa
     5521 ctttcctaca atgatctaaa tttgctaata aggaatatct atgcctaacc aagagctttt
     5581 agcccattca ttttgcccca aatgtggcgg cactttatgg atagtagata aaagcaatag
     5641 caatgctaaa ttctgggcac gtaacccaca agaaattttg tgtgtgaatt ctccaaaatg
     5701 cgattatcgc cgtaaagtaa cagaggatga tcgaaaaacg atagctcaaa atatttcttg
     5761 ttttgaagat tccagtctgt cttttgtgta ttgagaggtc taacaagttc ggggtttccg
     5821 cggtggcggt cctgccttac ccaacaggaa tttccacgct tctcctacct gtcttggtta
     5881 ccgccactgc gttttaaaaa tgaaaggatg ccattgtgaa acctaaaaaa cgatacgtag
     5941 tgttagctat cttgctttct ttaatgataa atgcatgtga tgctagcgat gttaacagca
     6001 tagttgacga atctgcagta aatgatatct atacacaagt cgctttaaca attacagctc
     6061 aagctcctat tattacagtt acaccgcatg caacatctac accatttcct acagcttctg
     6121 gagtaagttt gggattgaag acatcaatcc ctagccctgt ttccgtctca tacaacagtg
     6181 gaaatagtgg tgctgtaggt tgcgacaatt catcatattt gagtgatgcg accatcccag
     6241 atgggactgt actcgccccc ggcactgtat ttactaaaac gtggagtctg caaaatacag
     6301 gttcttgcga atggacaacc tcttacgcca tggctttcta cagcggcaat gcaatgagtg
     6361 gaacaacaac tgctcttagc gaaacagtta gcatcggttc aagcggggat atctctgtcg
     6421 aactgacagc cccatctact gcagggacgt atacagggta ttggagactc caaaatgcat
     6481 ctggaacttc atttggggaa gccgtgtatg tccaaatcgt ggtttcgggt agcacttcca
     6541 cacctacagc aacgccaaca tctacaagcg aagatgacga atcgactaat actcccaccg
     6601 caactaccgc acctactgaa acatccttac caacagaaac accagttcct acagaaactg
     6661 ttgagtcgta actttgttta taagacagag cgctacttac tgatttttac tttgacccat
     6721 tttctgagtg agttgattaa gaacaccgct tcaaactccg cccaatcttg gattttaagt
     6781 acccgctctt ctacctcgcc tgaagcgact agttccgccc gaaaggtgcc tgctaataaa
     6841 ccacattcaa cgggtggggt aaataacatt ccatctatct ttgcaaccaa attgccaatc
     6901 atgaactcgg ttaattcgtc tttttcgtta tataaaagca catcatcgaa acctgttata
     6961 gcggcttttt cgtacacttc tcggtttgtg gttttatgaa acaagaagcg atccatagaa
     7021 ttgacaggtt cttttgcaag acaaacttga aaaactttcg cacttggctg aaagtctttg
     7081 atttctgttg taataaatcc ttcagaattc agcgatattt ttatgcgttt ggatgaattt
     7141 ccttttgggc ttatagcatt caaggctttg aggaaatttt ctttcaagtt ttctgttttg
     7201 aagccaaagt agtttgccga atctatgatg cgtgcaatgt ggcgatcgat tagaaaataa
     7261 ccttcctcag gcatccaaag cacggtttca aatagagaga attctttttg tggtggggaa
     7321 gttaaaacct gagccttaag cagagcttca ctatattcat ctgcagacgt tgagtcccag
     7381 acaatacctc cgccaattcc atattctgct tttctatttt gcttatccac caaagccgtg
     7441 cggattgcta cattaaatcg agcttttcga cccggagcca tgtatccaat gcttccgcaa
     7501 tatatgtttc gtgaactggt ttccagatcc gcaatgatgt tcatcgtgct gacttttggc
     7561 gcacccgtaa tagacgcgca cggaaatagg tttacaaaga tttctgctac agatgccttt
     7621 gtttttcctt tcacagtgga tgtcatctga aagagggtgg gatatttttc gatagtaaac
     7681 aattctggta catggacact tcctaaatcc gcaattcgcc cgatgtcgtt gcggatcata
     7741 tccacgatca tcacattttc agccctgttt ttggaagatg cgcgtagcca attttgggca
     7801 tttgtatcct cttctgtatt ccgaccgcgc ttgattgtgc ctttcatggg gcgacctgtg
     7861 atggtatcgt catctaaatc gaagaacagt tcatgagagg cagaacaaat tgcccaatta
     7921 ccgatatcta aaaacgcagc atatttattt tgagaatggg taaggtgcct gaatagctgt
     7981 agcgcttgta aatcaatttc actttgttgg taggctttaa tatctgttga caaacgcata
     8041 gtgtaattaa cttgatacgt cttgccttgg gcgatttgat tttttataga ctgaatcgcg
     8101 ttgttataag cttcgcgatc aatatcagga cgccattcca aaggtaaaag agtgccataa
     8161 tcttctaatg agtctatggt ctcagaggtt tcatatagcc caaaccacaa aagcggaaac
     8221 ccatcttttg aagaaatcac ccttaatgca cggtcaaagg caggagaagc ttcataactt
     8281 acaaaccctg ctgcggtcca gccattttcg ttgaccagcc tttctacttc ttccaagcta
     8341 ttgcgaactt cgtctagctt ctcggttaca atcacgcggc gtggaccagt aaactttagc
     8401 caggcattgt ctttccgtaa aattacatca ccgatcaaag atacctctca tgaaaaagat
     8461 tcttgatatt gtaccgcgat ggtttcccgc ctttttaatg atggcgatta tttt
//
LOCUS       DAOQJE010000022         8505 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_4477_length_8505_cov_6.924615, whole genome shotgun sequence.
ACCESSION   DAOQJE010000022 DAOQJE010000000
VERSION     DAOQJE010000022.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 8505)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8505)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8505
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4477_length_8505_cov_6.924615"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            66..374
                     /gene="rpsJ"
                     /locus_tag="PLT08_01090"
     CDS             66..374
                     /gene="rpsJ"
                     /locus_tag="PLT08_01090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012084982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HNE03082.1"
                     /translation="MAKQKIRIRLKAYDHRVLDQSAKRIVETAERTGAHVVGPVPLPS
                     KKERYTIRRSSFIDKDSHEHFEIRTHNRLIDVLDPDSKTIDMLMRLNLPAGVDIEIKI
                     "
     gene            557..1189
                     /gene="rplC"
                     /locus_tag="PLT08_01095"
     CDS             557..1189
                     /gene="rplC"
                     /locus_tag="PLT08_01095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HNE03083.1"
                     /translation="MLKGLIGRKIGMTQIFDEQGVAYAVTLIEAGPCFVTQVRTAEKE
                     GYSAVQLGFGETNPKRLTQGQVGHLKANEITPLRYLREFRAKEHGLKVGDKVSVGDAF
                     AVGERVDVIGISKGKGFAGGVKRYHFHGMNATHGTSDRNRAPGSRGSGTTPGRVYKGA
                     RGAGHMGVDRVTAQNIKVVMVDPERNLIAINGAVPGGKGSLVMIKESRKQ"
     gene            1217..1846
                     /gene="rplD"
                     /locus_tag="PLT08_01100"
     CDS             1217..1846
                     /gene="rplD"
                     /locus_tag="PLT08_01100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HNE03084.1"
                     /translation="MKVDVLDLKGNKVREVELPANVFEAPVNVDLMHQAYTRQMANAR
                     LGTHDTKVRGEVRGGGRKPWKQKGTGRARQGSRRAAQWVGGGRIHTPHPRSYELRMPK
                     KMRLAALRSALSVKASEASLVVVEELNFDEPKTKVVAQALGNLVGSSTALVLMPAKDQ
                     NYDVVMRSASNIESAKVLLASYLNVRDILNFEKIVLPAKTIDALVAHLG"
     gene            1856..2161
                     /gene="rplW"
                     /locus_tag="PLT08_01105"
     CDS             1856..2161
                     /gene="rplW"
                     /locus_tag="PLT08_01105"
                     /inference="COORDINATES: protein motif:HMM:NF004363.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HNE03085.1"
                     /translation="MSDLYNVLVRPLVTEKSNTQSSKFGHYVFVVNNNATRTAVKDAV
                     ETLFDVTVARVNIVNAPAKRGRRGKSRRMVIRRPAFKKAIVTLAEGSKPLEIFEGVQ"
     gene            2161..2997
                     /gene="rplB"
                     /locus_tag="PLT08_01110"
     CDS             2161..2997
                     /gene="rplB"
                     /locus_tag="PLT08_01110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001738551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HNE03086.1"
                     /translation="MAVKKYKPITPGMRDMTGYTFEEITKSTPERSLLVIKKRSGGRN
                     AYGRVTVRHRGGGARRYIRVVDFKREKFGIPAKVASIEYDPNRTARLALLHYADGEKR
                     YIVAPVDLKVGDTLMSGPNAEIRSGNSLPISNIPVGTLIHNIEMKPGKGGQLVRSAGG
                     AAQLLAKEGDFAQVRMPSGEVRLIFQVCYATIGQVGNLDHSNVKLGKAGRKRHLGIRP
                     TVRGTAMSPRDHPHGGGEGRQPVGLPGPKSPWGKPTLGKKTRLNKKTDQYIVRRRSKT
                     NR"
     gene            3016..3288
                     /gene="rpsS"
                     /locus_tag="PLT08_01115"
     CDS             3016..3288
                     /gene="rpsS"
                     /locus_tag="PLT08_01115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007285290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HNE03087.1"
                     /translation="MSRSLKKGPYIEAKLLKRIEAMNQKKEKKVVRTWSRASVIFPQM
                     VGHTIAVHDGRRHVPIYITENMVGHRLGEFAPTRTFRGHQAAEKAA"
     gene            3301..3642
                     /gene="rplV"
                     /locus_tag="PLT08_01120"
     CDS             3301..3642
                     /gene="rplV"
                     /locus_tag="PLT08_01120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HNE03088.1"
                     /translation="MTDIQAHVRFLPQSAQKVRAVIDVVRGKSAIEALEILKFVNKRA
                     AGPVYKLVASAVANAEENFGVSRDDLFVAKITADEAPTRKWRRFGARGRFKPLLRRSS
                     HVTVILRERGA"
     gene            3652..4401
                     /gene="rpsC"
                     /locus_tag="PLT08_01125"
     CDS             3652..4401
                     /gene="rpsC"
                     /locus_tag="PLT08_01125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HNE03089.1"
                     /translation="MGRKVHPVGFRLGINQPWGGRWFAEGSTYRQQLHQDLAIRGLLM
                     GKLSKGGAAANEKVRELRKELPDAIRDAKAGISHVDVERTPGKIRIAIHTAKPGILIG
                     RKGEGVKKIRTALEALVGKKIELDIKEITNPDLDARLVAKNIADQLERRIAYRRAMKR
                     AIQQAMKQGAEGIKILVGGRLGGAEMSRDVWLREGRVPLQTLRANLDFATDEALTTYG
                     QIGVKVWIYKGEVAPEVEEKTEATEGVYVSE"
     gene            4433..4852
                     /gene="rplP"
                     /locus_tag="PLT08_01130"
     CDS             4433..4852
                     /gene="rplP"
                     /locus_tag="PLT08_01130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018133712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HNE03090.1"
                     /translation="MLMPKRVKWRKQMRGRMRGKALRGAEVAFGEFGLQALEPGWITA
                     RQIEAARRSIVREMKRRGKIWIRIFPAKPFTHKPPETRMGSGKGNVEYYVAVVKPGRI
                     MFEVAGLSADIAIAALRSAQYKFSIKTKVVARHAGGE"
     gene            4855..5076
                     /gene="rpmC"
                     /locus_tag="PLT08_01135"
     CDS             4855..5076
                     /gene="rpmC"
                     /locus_tag="PLT08_01135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HNE03091.1"
                     /translation="MKAMKSAEIRKLGLEEIRTKISDARDELMKLRFQQVTGQLTDTS
                     RLNILRKDIARMETILAEMLRNAAVEGAK"
     gene            5073..5345
                     /gene="rpsQ"
                     /locus_tag="PLT08_01140"
     CDS             5073..5345
                     /gene="rpsQ"
                     /locus_tag="PLT08_01140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HNE03092.1"
                     /translation="MNNRRRITGVVTSNKMTKTVVVEITRKYRHPLYKKVVFSSMRVK
                     AHDEIGCQIGDEVRIVESRPLSRDKRWVVESVVKREVRTADQGVGE"
     gene            5349..5720
                     /gene="rplN"
                     /locus_tag="PLT08_01145"
     CDS             5349..5720
                     /gene="rplN"
                     /locus_tag="PLT08_01145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007073026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HNE03093.1"
                     /translation="MIQHESRLKVADNTGARELLVIHVMGGSRRKYGAIGDVVVATVK
                     AAAPQGSVKKSEVVKAVIVRCTKEWRREDGSYIRFDDNAAVILDADGENPRGTRIFGP
                     VARELRDMGYMKIVSLAPEVL"
     gene            5730..6059
                     /gene="rplX"
                     /locus_tag="PLT08_01150"
     CDS             5730..6059
                     /gene="rplX"
                     /locus_tag="PLT08_01150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HNE03094.1"
                     /translation="MKVKIRKGDTVEVISGRLEDKGKRGEVINVILEERRVVVQGVNV
                     RTKHQKQVQAGNRQMNPGRVQFEGPIDISNVMLVCPKCSEATRVGVQRDDEGAHRVCK
                     NCQATID"
     gene            6078..6629
                     /gene="rplE"
                     /locus_tag="PLT08_01155"
     CDS             6078..6629
                     /gene="rplE"
                     /locus_tag="PLT08_01155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016281718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HNE03095.1"
                     /translation="MNKMQERYNKEVAPALLKSFGFKNVMQVPRLEKIVVNIGLGEAL
                     DNPKALEAAVTDLTTITGQKPVQTKARKSIANFKLREGRLIGTKVTLRGPRMWAFFDR
                     LVNIALPRVRDFRGISPNAFDGRGNYTLGLKDQLVFPEIEYDKIDKLRGMEITIVTSA
                     QNDDEARALLRQLGMPFSGKKEA"
     gene            6633..6815
                     /locus_tag="PLT08_01160"
     CDS             6633..6815
                     /locus_tag="PLT08_01160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003948630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type Z 30S ribosomal protein S14"
                     /protein_id="HNE03096.1"
                     /translation="MAKKCMQYREQRRRHAVQVRNRCQRCGRPRGYIRRFGLCRICFR
                     ELALKGQIPGVVKASW"
     gene            6836..7249
                     /gene="rpsH"
                     /locus_tag="PLT08_01165"
     CDS             6836..7249
                     /gene="rpsH"
                     /locus_tag="PLT08_01165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009754342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HNE03097.1"
                     /translation="MSFTDPIADMLTRIRNAVMAGHAQVALPSSKIKLEIAKILKEEG
                     FLEGFEVVDGETEAQKVLRLKIKYVGERRQRRAVISGLERVSKPGRRIYTKKSDIPWV
                     LSGIGVAILSTPKGVMTGARARQLGVGGEIICKVW"
     gene            7260..7808
                     /gene="rplF"
                     /locus_tag="PLT08_01170"
     CDS             7260..7808
                     /gene="rplF"
                     /locus_tag="PLT08_01170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HNE03098.1"
                     /translation="MSRIGRLPVDIPSGVQVELSGSKVRVKGPKGELSRVFSSLIDIK
                     MEDNQLKIARASDNPEERALHGTTRAVLANMIHGVSKGFEVILEVEGVGYRAEMEGKN
                     LALFVGYSHPVKIEPPAGIAFETDAKTRQIKIQGFDKELVGQVAANVRNVRPPEPYHG
                     KGLRYLGEKVRRKAGKAGKGAK"
     gene            7812..8177
                     /gene="rplR"
                     /locus_tag="PLT08_01175"
     CDS             7812..8177
                     /gene="rplR"
                     /locus_tag="PLT08_01175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015302640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HNE03099.1"
                     /translation="MANKNRALARERRHARVRKQVFGNPERPRLSVFKSLTGIYAQII
                     DDMDGNTVVSASSVDKELREKVKGMKKIEQAKAIGQAIAERAKSKGISSVVFDRGGFR
                     YTGRIKALADGAREGGLQF"
     gene            8187..>8505
                     /locus_tag="PLT08_01180"
     CDS             8187..>8505
                     /locus_tag="PLT08_01180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE03100.1"
                     /translation="MADNQFDKQYESQDAFEERVIEIARVAKVVKGGRRFRFRVTVVI
                     GDKKGNIGMGVGKANAVPDAMRKASERARKDIRTVNIAGTTIPHESLGKVAGAKVFLK
                     PASA"
BASE COUNT         2030 a         2134 c         2426 g         1915 t
ORIGIN      
        1 cgtgaaggcg gtctcaccgt cggtgcgggt gtcgtcacca agatcattgc gtaaggcgga
       61 gagaaatggc taaacagaag attcgcatcc gcctcaaggc ttacgatcat cgcgttctcg
      121 accaatccgc caagcggatt gtcgagaccg ctgaacgcac aggcgcccat gtagtgggtc
      181 ccgtaccctt gccaagtaag aaagaacgct atacaatacg gcgctcttcg ttcattgaca
      241 aggactccca tgagcacttc gagatccgca ctcacaatcg cttgatcgat gtgttggatc
      301 ccgactcgaa gacaatcgac atgctgatgc gcttgaattt gcccgctggt gtcgatatcg
      361 aaatcaaaat ctagttgaat aacacgtaat tttggttatt tccaaaatta cgatacgtta
      421 gagacggcgc aagagttggt ttgcgcgcga acaaagggtt cgcctcgcaa accaactgac
      481 tgtgccgcac ggagatgatg cggtcgttgc cccgactggc agtctcgtca atatcttgta
      541 aaggagaaaa ttgagtatgt tgaaagggct gataggtcga aagatcggca tgacccagat
      601 cttcgatgag caaggcgttg cctatgcggt aaccctcatc gaagctgggc catgttttgt
      661 tacccaggtc cgcacagctg aaaaagaggg atattctgca gtgcaacttg gttttggcga
      721 aacaaatccc aaacgtctca cacagggaca ggttggacat ttgaaagcca atgaaattac
      781 ccctctgcgt tatctccgcg aattccgagc caaagagcat ggtttgaaag tcggcgacaa
      841 agtttctgtc ggcgacgctt ttgccgttgg cgaacgtgtg gatgtgattg gtattagcaa
      901 gggtaaaggt tttgctggcg gtgtgaagcg ttaccacttc catggtatga acgccactca
      961 cggtacatct gaccgcaatc gtgcccctgg ttcacgtggc tccggtacga caccgggtcg
     1021 tgtgtataag ggcgctcgtg gtgcaggtca tatgggcgtg gaccgcgtaa ctgcgcagaa
     1081 cattaaggtc gtgatggttg atccggaacg caatctgatt gccatcaatg gcgctgttcc
     1141 cggcggcaag gggagcctcg tcatgatcaa ggaatcgcgc aagcaatagc gcgcaggaaa
     1201 tcggagcgag aataccatga aagtagatgt tcttgatttg aagggcaaca aggttcgcga
     1261 agtggaactc cctgcgaacg tgtttgaagc ccctgtgaat gtagatctca tgcaccaggc
     1321 ttacactcgc cagatggcga atgcccgcct cggtacgcat gatacgaagg tccgtggaga
     1381 agttcgcggt ggtggtcgca aaccttggaa gcagaaggga accggccgtg cccgtcaggg
     1441 ttcacgccgt gcggctcagt gggtcggcgg tggtcgtatc cacaccccgc atcctcgcag
     1501 ctatgaactt cgcatgccca aaaagatgcg ccttgcggct ctccgctcgg ctttgtctgt
     1561 gaaggcttct gaagcttctc tcgttgttgt ggaagaactc aacttcgatg agcccaagac
     1621 taaggtcgtg gctcaggcgt tgggtaattt ggtcggatct tccaccgctt tggtgttgat
     1681 gccagccaag gaccagaatt acgatgtggt gatgcgcagt gcaagcaata ttgagagcgc
     1741 caaagtgttg ctcgcaagtt acttgaatgt gcgtgacatc cttaattttg agaaaatcgt
     1801 tttgcccgcc aaaacgatcg acgcgcttgt tgcgcaccta ggataggaga gagacatgag
     1861 cgatctttat aatgtcctcg tccgcccgct ggtgaccgaa aagtctaaca cccagtccag
     1921 caagtttggg cactatgtgt ttgttgtgaa taacaacgcc actcgcaccg ctgttaaaga
     1981 tgcggtcgaa acgctctttg acgtcactgt ggcgcgcgtg aatattgtca acgcccctgc
     2041 aaagcgcgga cgccgcggca aatctcgccg tatggttatt cgtcgtcctg ctttcaagaa
     2101 ggcgatcgtt acccttgctg agggcagtaa gcccctcgag atctttgaag gggtgcaata
     2161 atggcagtta aaaagtacaa accaattacc cccggcatgc gcgacatgac cggttacacc
     2221 tttgaggaaa tcaccaagtc cactccggaa cgctctttgc tcgttattaa gaagcgctct
     2281 ggtggtcgta atgcttacgg tcgtgtgacc gttcgtcatc gtggtggtgg cgctcgtcgc
     2341 tacatccgcg tggtggattt caaacgcgag aagttcggca tccctgcaaa ggttgcatcg
     2401 atcgaatacg atccgaaccg cactgcgcgc ctcgctcttc tgcactatgc ggatggcgaa
     2461 aaacgctaca ttgttgctcc cgtggatttg aaggttggtg acacccttat gtcgggtccc
     2521 aatgctgaaa ttcgctctgg caactctctt ccgatcagca atattccggt cggtacactc
     2581 attcacaata ttgagatgaa gcctggcaag ggcggacagc tcgtccgttc cgctggaggc
     2641 gcagctcaac ttctcgccaa ggaaggcgat tttgctcagg ttcgtatgcc ttctggcgaa
     2701 gtccgcctca tcttccaggt ttgttatgcc acgattggtc aggttggtaa tcttgatcac
     2761 agcaatgtca agcttggtaa ggctggtcgc aaacgccacc ttggtatccg cccaaccgtc
     2821 cgcggtactg caatgagtcc ccgcgaccat ccacacggtg gtggtgaagg ccgccagccg
     2881 gttggtcttc ccggtcctaa gtcaccttgg ggtaagccta cccttggtaa gaagacccgc
     2941 cttaataaga agacggatca gtacattgtg cgtcgtcgca gtaagacgaa ccgctagtag
     3001 atgaggtaag cagatatgtc acgatcattg aaaaaaggcc cgtacattga agcaaaattg
     3061 ctcaagcgta tcgaagccat gaatcagaaa aaagaaaaga aggtggttcg cacctggagt
     3121 cgcgcttctg tgatcttccc tcagatggtg ggtcacacga tcgcggttca cgacggacgc
     3181 cgccatgtac cgatctacat tacggaaaac atggttggtc accgtttggg cgaatttgcc
     3241 ccgacacgca cattccgcgg acatcaggcc gctgagaagg ctgcgtaagg agaataggac
     3301 atgactgata ttcaagctca cgttcgtttt ctccctcagt ccgcacagaa agttcgcgct
     3361 gttatcgacg tagtgcgcgg caagagcgcc attgaagcgt tggaaatcct caagtttgtg
     3421 aacaaacgtg ccgctggccc ggtttacaaa ctggttgctt ctgctgtggc gaatgctgaa
     3481 gaaaacttcg gtgtcagccg cgatgacctg ttcgttgcca agatcactgc agacgaagcc
     3541 ccgacccgca agtggcgtcg cttcggcgcc cgcggtcgtt tcaagccgct tttgcgccgc
     3601 agttctcatg taacagttat tttgcgcgag cgcggagcat aaggagatta tatgggtcga
     3661 aaagtacatc ccgttggttt tcgtctcgga atcaatcagc cgtggggcgg ccgttggttt
     3721 gctgagggaa gcacatatcg ccagcagttg caccaggatc ttgccatccg cggtttgttg
     3781 atgggcaagc tctccaaggg cggcgctgct gctaatgaaa aagtccgcga actgcgcaag
     3841 gaactccctg atgcaatccg cgatgccaag gctggtatct cccatgtaga tgtggagcgc
     3901 acccctggca agatccgcat cgccattcac accgccaagc ccggtatttt gatcggtcgc
     3961 aaaggcgaag gtgtgaagaa gatccgcact gcgctcgaag cgttggttgg taagaagatc
     4021 gaattggaca tcaaagagat cacgaatcct gacctcgacg caagactggt tgccaagaat
     4081 attgctgacc agctcgaacg ccgtatcgcg tatcgccgcg ctatgaagcg tgccattcag
     4141 caagccatga agcagggcgc cgagggcatc aagatcctcg ttggcggtcg cttgggcggc
     4201 gctgaaatgt ctcgtgatgt ctggctccgc gaaggacgtg ttcctctgca gaccttgcgt
     4261 gctaaccttg atttcgccac cgacgaagct ctgaccactt acggacagat cggcgtgaag
     4321 gtgtggatct ataagggtga agtggcaccg gaagttgaag aaaaaaccga agcgacggaa
     4381 ggcgtgtacg tcagcgagta atcgctgcgt cgcttgagat gaggttattg ctatgttgat
     4441 gcctaaacgt gtaaagtggc gcaagcagat gcgtggtcgc atgcgaggta aggctctccg
     4501 cggtgcggaa gtcgccttcg gcgaattcgg tctacaggcg ctcgaaccgg gttggattac
     4561 agcccgtcag attgaagcgg ctcgccgttc gatcgtccgc gaaatgaaac gccgcggtaa
     4621 gatctggatc cgtattttcc cggccaagcc gttcacacac aaaccacccg agactcgtat
     4681 gggttccggt aaagggaatg tggagtacta cgtcgctgtg gtgaagcctg ggcgtattat
     4741 gttcgaagta gcaggtttgt ctgcggacat tgccatcgct gctttgcgct ctgcccagta
     4801 caagttctcc atcaagacca aggtcgtggc tcgccacgcg ggaggagaat agtcatgaag
     4861 gctatgaaat ccgcagaaat tcgcaagctg ggtcttgagg aaatccgcac aaagatttcc
     4921 gatgcgcgcg atgagttgat gaaactgcgc ttccagcagg tgaccggtca gctgaccgat
     4981 accagccgct tgaacatcct ccgcaaggat attgctcgaa tggaaaccat tctggctgaa
     5041 atgctcagga atgctgcagt agaaggtgca aaatgaacaa tcgtcgtcgt atcactggtg
     5101 tagtcaccag caataaaatg accaaaacgg tcgtcgtgga aatcactcgc aagtatcgcc
     5161 atcctcttta taagaaggtt gtgttctcga gcatgcgcgt gaaagctcac gatgagatcg
     5221 gctgccagat cggtgatgaa gtccgcatcg tggaatcccg cccgttgtcg cgcgataagc
     5281 gttgggtggt cgaatccgtc gtcaagcgcg aagttcgtac tgccgatcag ggtgtaggag
     5341 aataagccat gatccagcat gaatctcgtt tgaaggttgc tgataatacc ggcgcccgtg
     5401 aacttctcgt catccatgtg atgggcggtt cccgacgcaa atatggtgct attggtgatg
     5461 tggtggtggc aactgtcaaa gctgctgctc cgcaaggctc tgtaaagaag agcgaagttg
     5521 tgaaagccgt gatcgtgcgc tgcaccaaag aatggcgccg cgaagacggc tcgtacatcc
     5581 gctttgacga taacgctgca gttatcctcg atgccgatgg cgaaaacccg aggggcacgc
     5641 gtatcttcgg acccgtggca cgcgaattgc gtgatatggg ctacatgaag atcgtgtcgc
     5701 ttgccccgga agtgctgtag gagctgagaa tgaaagttaa gattcgaaaa ggtgataccg
     5761 tcgaagtgat cagcggccgt ctcgaagata aaggcaagcg cggtgaagtc atcaatgtga
     5821 tccttgaaga acgccgtgtg gtggtgcagg gtgtaaacgt ccgcaccaag caccagaaac
     5881 aggtgcaagc tggcaaccgc cagatgaacc ctggtcgcgt tcagttcgaa ggtcctattg
     5941 atatttccaa cgtgatgttg gtctgcccga agtgcagcga agccacccgt gtgggtgtgc
     6001 aacgtgatga cgaaggcgca catcgtgttt gcaaaaattg tcaagccacg attgactagg
     6061 caccggagca ttagaacatg aacaaaatgc aagaacgtta taacaaagaa gtagccccgg
     6121 ctctgctgaa gtcattcggt ttcaagaatg tgatgcaggt tccgcggttg gaaaagatcg
     6181 ttgtcaacat cggtcttggt gaagcgttgg ataaccccaa ggctctcgaa gctgctgtga
     6241 ccgacctgac caccattacc gggcagaaac ccgtgcagac caaagctcgc aaaagcattg
     6301 cgaacttcaa actgcgtgaa ggtcgtttga ttggtaccaa ggtgaccctg cgtggtcccc
     6361 gcatgtgggc gttctttgac cgcctcgtga acattgcact tccgcgcgtg cgcgacttcc
     6421 gtgggatttc cccgaacgcg tttgacggac gtggaaacta tacgcttggt cttaaggatc
     6481 agttggtttt ccctgagatc gagtacgaca aaatcgataa attgcgcggt atggaaatca
     6541 caattgtgac gagtgcccag aatgatgatg aagcccgcgc tttgttaaga cagctcggta
     6601 tgccgttcag cggcaagaag gaagcgtaag gtatggcaaa gaaatgtatg caatatcgtg
     6661 aacagcgccg ccgccatgct gtgcaggtga gaaatcgctg ccagcgctgc ggacgacccc
     6721 gcggatacat ccgccgcttc ggcttgtgcc gcatttgttt ccgtgaattg gcgttgaagg
     6781 gccagatccc cggcgtcgta aaggcgtcct ggtaaaaccg ggttggaggt taatcatgtc
     6841 attcactgat ccaattgccg atatgttgac tcgcattcgc aacgccgtta tggcgggcca
     6901 tgcccaggtt gctttgccga gctcaaagat caagcttgaa atcgccaaga tcctcaagga
     6961 agaaggtttc cttgaaggtt tcgaggtggt cgatggcgaa accgaagcgc agaaagtttt
     7021 gcgcctcaag attaagtatg taggcgaacg ccgccagcga cgtgcagtta tctccggttt
     7081 ggagcgcgtc agcaagcccg gtcgtcgtat ctacaccaag aaatcagaca tcccctgggt
     7141 tctctctgga attggcgttg ccatcctttc gacccccaag ggtgtaatga ccggcgcacg
     7201 tgcccgacag ttgggcgtgg gcggcgagat catctgcaag gtttggtagg aggtttattg
     7261 tgtctcgcat tggtcgttta cccgtagaca tccccagcgg tgtgcaggtg gaattatccg
     7321 gctccaaggt ccgtgtgaag ggaccgaaag gtgagctttc acgcgtgttc tccagcttga
     7381 tcgatatcaa gatggaagat aaccagttga agatcgcccg cgcttcggat aaccctgaag
     7441 agcgtgctct acacggcacc acccgcgccg ttctcgccaa catgatccat ggcgtgagca
     7501 agggatttga agtgatcctc gaagtggaag gcgtgggtta ccgcgccgaa atggaaggca
     7561 agaatctggc tttgtttgtg ggctactccc acccggtcaa gattgagccg cctgctggca
     7621 tcgcttttga aacagacgcg aagacccgtc agatcaagat tcagggcttc gataaagaat
     7681 tagtggggca ggtggctgcg aacgttcgta acgtgcgccc gccagagccc tatcatggca
     7741 aaggcttacg ctatctcggc gaaaaggttc gccgcaaagc tggtaaagcc gggaaaggag
     7801 ccaagtaatc catggctaac aaaaatcgtg ctctggctcg tgaacgccgt catgcgcgtg
     7861 ttcgcaagca agtgtttgga aatcccgaac gcccgcgctt gagcgttttc aagagcctta
     7921 cgggtatcta tgcccagatc atcgacgaca tggatggaaa cactgttgtt tctgcctcca
     7981 gcgttgataa agaactgcgc gaaaaagtga aaggcatgaa gaagatcgaa caggctaagg
     8041 ccatcggtca ggcgattgct gaacgcgcga agtccaaggg aatttcttcg gtcgtatttg
     8101 accgtggtgg tttccgctac accgggcgta tcaaagccct ggcagatggc gcgcgcgaag
     8161 gcggcttgca gttctaggag aaggaaatgg ctgataatca atttgacaaa cagtacgaat
     8221 cgcaggatgc cttcgaagaa cgcgtgatcg aaatcgcccg cgttgccaaa gtggtgaaag
     8281 gtggtcgccg cttccgcttc cgcgtgaccg ttgtgattgg tgacaagaag ggcaatatcg
     8341 gtatgggtgt tggtaaagcc aatgctgtgc ccgatgcaat gcgcaaggct tctgaacgcg
     8401 ctcgcaagga cattcgcacc gtcaacattg ccggaacgac catcccacat gagtcgctcg
     8461 gtaaagtagc tggcgccaaa gtcttcctta agcctgcatc cgctg
//
LOCUS       DAOQJE010000023         8367 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_4598_length_8367_cov_6.947907, whole genome shotgun sequence.
ACCESSION   DAOQJE010000023 DAOQJE010000000
VERSION     DAOQJE010000023.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 8367)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8367)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8367
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4598_length_8367_cov_6.947907"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..903)
                     /locus_tag="PLT08_01185"
     CDS             complement(<1..903)
                     /locus_tag="PLT08_01185"
                     /inference="COORDINATES: protein motif:HMM:NF024873.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease H-like domain-containing protein"
                     /protein_id="HNE03101.1"
                     /translation="MSSLADKLKSLGVKTANTLPTPPKPESQSIDSVVAGSFISTPRG
                     EAFVAEQAYDPTYVHGRFSPFSSFPLSFISQWANDPRIDQLPITKYAFLDTETSGVSG
                     GTGTYAFLVGAARFVDGKFVIKQFFLRDPAEEPAMLEALINFLAPCEGLVTFNGKAFD
                     APLLVTRYSLHRIPVPFKNYAHIDLLPLARRLWRDRLPSRALKYIEEHVMGFTRSSEE
                     VPGYEIPWMYFDYLRTGDARPLGGVFYHNAMDVVAMAALLAHVSEILADPYNGRVEHG
                     LDFIALGKLFEDLGHWDEAARLFER"
     gene            961..2388
                     /locus_tag="PLT08_01190"
     CDS             961..2388
                     /locus_tag="PLT08_01190"
                     /inference="COORDINATES: protein motif:HMM:NF024742.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNE03102.1"
                     /translation="MSRKLPFWLKTLYGSGDWGIASISMMRSIFYAIYLTDVVGIEPR
                     LASLGALVGIVWDAVNDPIIGMLSDRVKSKLGRRRPFLLWFAFPFGLSFVMLWSAPNW
                     DSQVALLIYVTLAFMISDTLTTLVAVPYYSLTPELTQNYDERTSLSSFRSVFQLLSAM
                     VVVVSAPMIVDAVISGGGTQQQGFLTAGAVFGAIGSLPLFLIGLFIRERFAEDTQEQE
                     ELSFRETLKLAWQNVPFRYAVGIYMFNWSAVDMVSITFPFFLLYWVAQGDLLAKINIL
                     GVDLALESAFFGVLMLVCVLFVPFWLWLSKRQNKIRAYILGMAAWVVVQVLIFTIQPG
                     ETSYMLFLAGLAGIGVSAAYILPDSILPDVIEWDELRTGRRQEGIYYGIRTLIRKLTG
                     ALIIFITLQILGWSGYQAPPENVTVYQQPESALFMIRLMDSFIGAAILAGTIILAWSY
                     PLTREKYQKIQKLLAVRRNKNAETT"
     gene            complement(2424..4103)
                     /locus_tag="PLT08_01195"
     CDS             complement(2424..4103)
                     /locus_tag="PLT08_01195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase family protein"
                     /protein_id="HNE03103.1"
                     /translation="MYDKKKLDEIQQSLSKWEETSLKKALNQLPERADEFITTSSEPI
                     NRLYTPLDTADLDYNDSLGNPGEYPYTRGVHPTLHRSKLWTMRMFAGFGTAEETNGRF
                     KYLLEQGQTGLSIAFDLATLMGYDTDQPEALGEFGKCGVAVSSLKDMEILLDGIPLDK
                     VSSSMTINSPASIIWAMYLVAAEKQGVRLDQLRGTLQNDILKEFIAQKEFIFPPEPSM
                     RLVVDTMEFGSKHVPQWNTISISGYHIREAGSTAAQELAFTLADGMEYVRWGIARGMD
                     VDEFAPRLSFFFNAHNDFFEEIAKYRAARRIWAREMRETFKAKNPRSWLMRFHTQTAG
                     VSLTAQQPENNIVRVAIQALAAVLGGTQSLHTNSMDEALALPSEHAVKVALRTQQIIA
                     EESGVTNTVDPLGGSFFVEAQTDRMEKDAYAYFQRIQDLGGVIPALEKGFMQSEISDA
                     AYRYQREIDENRRKIVGVNAYLDEKPAAIPILKMDPNGYSRQMNRLNEVRKTRDSGRV
                     GQTLDALRIACEGTENTMPYILDCVRAYCTLGEIIGVMTKVFGKYEEPTMI"
     gene            4259..4786
                     /locus_tag="PLT08_01200"
     CDS             4259..4786
                     /locus_tag="PLT08_01200"
                     /inference="COORDINATES: protein motif:HMM:TIGR03847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3090 domain-containing protein"
                     /protein_id="HNE03104.1"
                     /translation="MSRTDIDVDPCDHITADAIGKPGQRVFYLHAYSEHRTYTIIIEK
                     AQLLSLAIGVEQFLGQLSQQYPDLEEASGDYVEEVMRINPPVDPLFRAGEIGLGYDKD
                     RDRVVVFAKELITEEQDPEEASQIRFWATRTQLRQLARWGQEVSQRGRPICPQCGQPM
                     EAGGHFCPKKNGHLH"
     gene            4827..5522
                     /locus_tag="PLT08_01205"
     CDS             4827..5522
                     /locus_tag="PLT08_01205"
                     /inference="COORDINATES: protein motif:HMM:TIGR03843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCO1664 family protein"
                     /protein_id="HNE03105.1"
                     /translation="MTNIKTILTHGNYELRGQFMLGSNYTFLVDVHHEDEVVKAVYKP
                     SKGEQPLWDFPDNTLALREVAAYELSETLGLHIVPVTVYRDDGPHGPGSLQQYIEYDI
                     EYHYFNFTVEDKQKLRPVVFFDILANNADRKGSHVFFEDETHRLYAIDHGICFHEENK
                     LRTVLWDFAGQAIPDELLAPLSLTGNWSALFEPYLSPREIDALLFRAEELCESKVFPR
                     PLQGRRAYPYPPI"
     gene            5643..6677
                     /locus_tag="PLT08_01210"
     CDS             5643..6677
                     /locus_tag="PLT08_01210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine dehydrogenase"
                     /protein_id="HNE03106.1"
                     /translation="MHYNIALIGFGNVARSLARLLVRKQDVLKSKYDVTFSFTGISTG
                     RHGYAVNQSGLDIQKALDLVESGKDISELSVFPVNDSISVINNSAANVMFENSPVNTQ
                     TGQPAVDHIRTALNLGMHAITANKGPVVHGYRELTALAESKGKTFRHESAVLGGAPVF
                     SVMREAFPLADLESFKGIFNATTNVILSRMESGETYEQALAYAQKIGLAETDPTNDVD
                     GWDAAIKVASLVTVLWDTPMTPQQVNPTGIRGITSEMIANAKKEGKRYKLVCSAERTD
                     GKIVASVSPQLVDSSSSLYGMMNSSTGITFRTDVIIDYSIVLSEKPGMTGGPVETAYG
                     LFADFVNIVR"
     gene            6756..8294
                     /locus_tag="PLT08_01215"
     CDS             6756..8294
                     /locus_tag="PLT08_01215"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:NF028237.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HNE03107.1"
                     /translation="MTPPNILYSTALLTAGICCFIVGLLVLQRRPFMPGSIPLMVFIL
                     AMCWWDISYGIFWGGVLKPSPYFWLDISYIGVVIVPAAFFVFAMQISGRQEWLKTPLL
                     IGLYVEPLLVLVLMWTDPWHNLFFAGKRLENTGMILNAGPVFWANVVYSYLLLLVTFI
                     LLVRRFTTTSGIYRRQLGVVIAGAGVTWLNSFIFVTGLSPFPNADNTPFSFTIAAFAF
                     AYALLRYRMLDIVPVARDTLIEGMSDGVLVLDTNNRIVDMNPEAEKLLSASSAGTIGR
                     HVEEVFAPWADLVKTFMNVDQTRVEVLASRDSDTYLDLQISPLFDKRKNFIGRLVMWR
                     DISRLKKIQTELEKLATQDPLTHVYNRRHFYTLAEVEIHRSLRSQLPFSVILVDIDYF
                     KSINDKYGHKAGDEALIHFTKICIQNLRKVDIFARFGGEEFVILLPEADLDQAKETAE
                     RIRKIVESTVLNIDEHQIKITASFGVSTFKGSPDTLDLLLQKSDEALYAAKNTGRNRV
                     VCAD"
BASE COUNT         1999 a         2012 c         2079 g         2277 t
ORIGIN      
        1 acgctcgaac agacgtgccg cctcatccca atgtccaagg tcttcaaata gtttccctag
       61 tgcaatgaaa tcaagcccat gctccacacg tccattgtag gggtctgcca aaatctcgct
      121 gacgtgtgcc agcaacgccg ccatcgctac cacatccatg gcattgtgat agaacacacc
      181 tccaagtgga cgggcatcgc ctgtgcgtaa gtaatcgaaa tacatccacg gaatttcata
      241 accgggtact tcttcagaag aacgcgtaaa ccccattaca tgttcttcga tatatttcaa
      301 tgcacgcgaa gggagtcggt ctctccagag tcttcgtgct aaaggcagta aatcaatatg
      361 ggcataattc ttaaaaggga cagggattct gtgcaacgag tatcgagtaa cgagaagtgg
      421 tgcgtcgaaa gctttgccat tgaaagtaac caacccttca caaggcgcta gaaaattgat
      481 aagcgcttca agcatggcag gctcttcagc cggatctcgc aagaagaatt gcttgatgac
      541 gaacttgcca tctacaaaac gggcggcacc cacgaggaaa gcgtaggtac ccgtcccacc
      601 agagacgcca gacgtttcag tatctaaaaa ggcatacttg gtgatgggga gctggtcaat
      661 gcgtgggtca tttgcccatt gcgaaataaa cgaaagcgga aacgatgaga acggtgaaaa
      721 ccgcccgtga acataagtgg ggtcataggc ttgttctgcc acaaaggctt cgccgcgcgg
      781 cgtggagata aaactcccag cgacgactga atcaatggat tggctctcag gcttgggcgg
      841 ggtgggaagc gtgtttgccg tcttcacgcc gagtgattta agtttatccg ctaaggagga
      901 catgtctaat attttatcgc tattaaccgt gatacacttg cgcgaaattt tgaaggtgcg
      961 atgagcagaa aactcccatt ttggttgaag actttatatg gttcgggtga ttggggaatt
     1021 gccagcatta gtatgatgcg ctcgatcttt tatgccattt acttgacaga tgtggttggc
     1081 attgagccac ggctggcatc gttgggtgca ttggttggaa ttgtgtggga tgccgtcaat
     1141 gacccgatta ttggcatgct gagtgacagg gtgaaatcaa aacttggcag gcgtagaccg
     1201 ttcttgttgt ggttcgcctt cccattcggg ttgagttttg tgatgttatg gtctgcaccg
     1261 aattgggata gtcaggttgc tttgttgatc tatgtgacgt tggcgttcat gatctcagac
     1321 acgctgacaa cgttggtggc ggttccatat tattcgctca cccccgagtt gactcaaaac
     1381 tatgatgagc gtacatcgct ctcgagtttt cgttcggtct ttcaattgct ctctgcgatg
     1441 gtagtggtgg tttctgcgcc catgatcgtg gatgccgtga tttcaggcgg cggcacgcaa
     1501 caacaaggtt ttctcacggc tggggcggtg ttcggcgcga ttggttcgtt gccattgttc
     1561 ttgattggtc tttttatacg tgaacgattt gcggaagaca cacaggaaca agaggaactt
     1621 tcatttcgtg agaccctgaa actggcttgg cagaacgtgc catttcggta tgcagtggga
     1681 atttacatgt tcaattggtc tgccgtggac atggtttcga tcaccttccc attcttcctt
     1741 ttgtattggg tggcgcaggg agatttattg gcaaagatca atatccttgg tgtagacctt
     1801 gctttagagt ctgccttctt tggggtgttg atgttggttt gtgtgttgtt cgtgccgttc
     1861 tggctgtggc tatcgaaacg acaaaataag atcagagcgt acatccttgg tatggcggcg
     1921 tgggttgttg tgcaggtgtt gatcttcact attcaaccgg gcgaaaccag ctacatgttg
     1981 ttccttgccg ggttggctgg tattggcgtt tctgccgctt acattttgcc agactcgatt
     2041 ttgccagatg tgattgaatg ggatgaactt cgtacggggc gcagacaaga aggtatttat
     2101 tacgggatcc gtacgctcat ccgtaagttg accggggcgt tgatcatttt catcacgctg
     2161 caaattttgg gttggtctgg ctatcaggcg ccgccagaaa atgtgaccgt gtaccaacaa
     2221 ccggaatctg ccttgttcat gattcgccta atggactcgt tcatcggcgc agcaattttg
     2281 gcaggcacga tcattctggc atggtcttat ccgctcacac gtgaaaaata tcagaagatt
     2341 caaaagttac tggctgttcg ccgaaacaaa aacgcggaaa cgacttgatc gtttccgcgt
     2401 tttgctataa aaggcgagag cgtctagatc atcgttggtt cttcgtactt tccaaaaacc
     2461 ttcgtcatca caccgatgat ttcccctaat gtgcaatagg cacgcacaca atccaaaata
     2521 tatggcatgg tgttctcggt cccttcgcaa gcgatgcgta aagcgtcgag agtttgccct
     2581 acgcgcccac tatcgcgcgt cttgcggact tcgttcaggc gattcatctg acggctgtat
     2641 ccgttcgggt ccattttgag gattggaatg gcggcaggtt tttcatcgag atacgcattg
     2701 acgcccacga tcttgcggcg gttctcatcg atctcacgtt gatagcgata cgccgcatcg
     2761 gagatttcgc tttgcatgaa acctttttcc aatgctggga tgactccgcc caagtcttgg
     2821 atgcgctgga agtaggcgta cgcatctttc tccattcggt ctgtttgcgc ttcgacgaag
     2881 aaactacccc caagcgggtc gaccgtattc gtcacgccgg attcttctgc gatgatctgc
     2941 tgagtccgca gagcaacttt cacagcatgt tcagagggca atgcaagcgc ttcatccatc
     3001 gagttggtgt gcaaactttg cgttccaccc aataccgcag ccagcgcctg aatcgccacg
     3061 cggacgatgt tattttccgg ctgttgtgcc gtgagcgaga cacccgctgt ttgagtgtgg
     3121 aagcgcatca gccacgagcg cggattcttc gctttgaagg tctcgcgcat ttctcgcgcc
     3181 caaatacggc gagccgcacg atacttggca atctcttcga agaaatcgtt atgagcattg
     3241 aagaagaaag agagacgcgg cgcaaattca tctacatcca tgccgcgtgc aatgccccag
     3301 cggacatatt ccatgccgtc tgcaagggtg aacgccagct cttgcgcagc cgtcgaaccc
     3361 gcttcacgga tgtgatagcc cgaaatggaa atggtattcc actgcggcac gtgttttgag
     3421 ccgaactcca tcgtatccac tacgagacgc atggaaggtt ctggtgggaa gatgaattcc
     3481 ttctgtgcga taaattcttt gagaatgtcg ttctgaaggg tgccgcgtaa ttgatcgaga
     3541 cgcacacctt gtttttcagc ggctacaaga tacattgccc agatgatgga agccggcgag
     3601 ttgatggtca tgctcgaaga aactttatcg agcggaatgc catccaaaag aatttccata
     3661 tctttaagcg aagaaacggc tacaccacat ttgccgaatt cacccagcgc ttcgggttgg
     3721 tcagtatcgt agcccatcag cgtggcaagg tcgaaggcaa tgctcaagcc ggtctgaccc
     3781 tgctcaagta agtatttgaa gcgcccgttc gtctcttctg cagtaccaaa gcctgcaaac
     3841 atacgcatgg tccacagttt ggagcgatgc aacgttgggt gaactccacg tgtgtatgga
     3901 tattcacctg ggttgccgag cgagtcgttg taatctaaat cggcggtgtc gagcggtgta
     3961 taaagtctgt tgatcggttc ggaggaagta gtaataaatt cgtcagcacg ttcagggagt
     4021 tgatttaaag cttttttgag agaagtctct tcccacttgg aaagcgattg ttgtatttca
     4081 tcgagctttt ttttatcgta catggcatct cctttggcga gttgatgaga ttataccgcc
     4141 ccctccttct actatcacgg gcatctcttc ccatatgtaa tgaattaact ttcctcacat
     4201 tttagggagg atgggtgggg atggctattc tggtatactc tcaaaaaagg taatctttat
     4261 gtctcgcact gatattgacg tcgacccctg tgatcacatc accgctgacg cgattggcaa
     4321 acctggtcaa cgcgtttttt atctgcatgc ttattccgag catcgcacct acactatcat
     4381 cattgaaaaa gcgcagttgc tttcacttgc cattggcgtg gagcaattcc ttggtcagct
     4441 tagccagcaa taccccgacc ttgaagaagc ttctggcgat tatgttgaag aagtcatgcg
     4501 catcaacccg cccgtcgacc cgctcttccg cgctggtgag atcggcttgg gctacgataa
     4561 agaccgcgac cgtgtagtcg tcttcgcaaa agaactcatc actgaagaac aagaccccga
     4621 agaagcgtcg cagatccgtt tttgggcgac ccgtacccaa ctgcgtcagc tcgcccgttg
     4681 ggggcaggaa gtctctcaac ggggtcgccc catttgcccg caatgcggtc aacccatgga
     4741 ggcgggcggt cacttctgcc ccaagaaaaa cggacacctt cactaacgtt taaacgtgaa
     4801 aacgttcgaa cgcttaaacg tttcccatga ccaatatcaa aaccatcctc actcatggca
     4861 actacgagct tcgtggtcag ttcatgttgg gctcgaatta cacattcctt gtagatgttc
     4921 accacgagga tgaagttgtt aaagctgtat acaaacccag caaaggcgaa caaccgcttt
     4981 gggattttcc cgataacacc cttgcgttgc gcgaagtcgc tgcatatgaa ctaagcgaaa
     5041 cgcttggctt acacatcgta ccagtcaccg tctatcgcga cgatggtccg catggacccg
     5101 gctctttaca gcaatacatc gaatacgata tcgaatatca ctacttcaac ttcaccgtgg
     5161 aagataagca aaaacttcgt cccgttgtct tcttcgatat tctcgccaac aatgctgacc
     5221 gcaaaggaag ccatgtcttc tttgaggatg aaacccatcg cctctatgcc atcgaccatg
     5281 gcatttgttt tcatgaggag aataaactcc gcaccgtatt atgggatttt gccggtcaag
     5341 ccattcctga cgaattgctc gcgcctctct cgttgaccgg aaactggtct gccctctttg
     5401 agccttatct cagcccgcgc gagattgatg ccctactctt tcgcgcagaa gaattgtgcg
     5461 agtcaaaagt ctttccacgc ccgttacaag gacggagggc gtatccttac ccgcccattt
     5521 aatgtcattg cgagggcgct cttgtttttt gcctgaagca atctgataac gtgttgaaga
     5581 ttgcttcgtt gggaagaaca ccttcctcgc aatgacggaa tacagtttca ggagaaattt
     5641 ttatgcacta caacatcgca ctcatcggtt ttggaaatgt cgcccgttcc ttggctcgtc
     5701 tgctggttcg caagcaggat gtcttgaagt cgaaatatga tgtcaccttt tcatttactg
     5761 gcatttcaac cggtcgtcac ggatatgccg tcaatcaaag tgggttggat attcaaaaag
     5821 ccttggactt ggtcgaaagc ggaaaagata tctctgagct ttctgtattc cctgtcaatg
     5881 actcgatcag cgtgatcaac aactctgctg ccaatgtgat gtttgaaaat tctcccgtca
     5941 atacccagac cggtcaaccc gccgtggacc acatccgcac ggcgttgaat ttgggaatgc
     6001 atgctatcac cgcaaataaa ggtccagttg tgcatggcta tcgcgagttg accgcgttgg
     6061 cggaatcgaa gggcaaaacg ttccggcacg agtctgctgt tttgggaggc gcacccgttt
     6121 tctcggtcat gcgtgaggcg tttccgctcg cggatcttga atccttcaaa ggcatcttca
     6181 atgctacgac caatgtcatc ttatctcgca tggaaagcgg cgagacgtat gaacaggcac
     6241 ttgcgtacgc acagaagatc ggtctcgctg aaaccgaccc caccaacgac gtggatggct
     6301 gggatgccgc tatcaaggtt gcatcgcttg tcacagtgtt atgggatacg cccatgaccc
     6361 cgcagcaagt gaatcccact ggcattcgtg gcattaccag tgagatgatc gccaacgcca
     6421 agaaagaagg caaacgctat aagttggttt gctcagctga aaggacggat ggcaagattg
     6481 ttgccagtgt ttcaccgcag ttggtcgatt catcttcgtc tctgtacggc atgatgaact
     6541 ctagcactgg catcaccttc cgcacagatg tgattattga ttactccatt gtcctctctg
     6601 aaaaacccgg tatgacgggt ggacccgtgg aaaccgcata cggtctcttt gccgattttg
     6661 tgaatatcgt ccgctaactg cccttttatt cgaatatcat tgttaagtag taaagtagag
     6721 cctttgtgtt tacaatagta gacacaaagg cttttatgac ccctccaaac atcttgtata
     6781 gtactgcact cttaacggct gggatttgtt gctttattgt tgggttattg gtgttacaaa
     6841 gacgcccgtt tatgccgggg tcgatcccat tgatggtttt catccttgcc atgtgctggt
     6901 gggacatttc gtatggcatt ttctggggcg gcgttttaaa gccttcacct tacttttggt
     6961 tggatattag ttatatcggg gtggttatcg tcccggcggc gttttttgtt tttgccatgc
     7021 aaatctcagg caggcaagaa tggttgaaaa caccattgtt gattgggttg tatgtagaac
     7081 cgttgttggt tcttgttctg atgtggaccg acccctggca taatcttttc tttgctggca
     7141 aacggttgga aaacaccggc atgatcttaa atgcaggacc ggtattttgg gcaaacgttg
     7201 tatattctta tctgttgtta ttggtcacgt tcattttgtt agtgcgtaga tttacaacga
     7261 cttctggaat ttatcgccgc cagcttgggg ttgtgattgc aggtgcagga gttacctggc
     7321 tcaatagttt tatttttgtc actggtttaa gccctttccc aaatgcagat aatacgcctt
     7381 tttcatttac gatcgctgcc tttgcgtttg cctacgcttt gctccgttat cgcatgttgg
     7441 atattgtgcc cgtggcgcgc gatacattga tcgaaggtat gagcgacggt gtgcttgttt
     7501 tggatacaaa caatcgtatc gtggatatga atcccgaagc ggaaaaactt ctctccgcct
     7561 cttccgccgg gacgattggc aggcacgtag aagaagtttt cgccccgtgg gctgaccttg
     7621 taaaaacatt tatgaacgtg gaccaaaccc gggtggaagt tctagccagc cgtgactcag
     7681 atacatatct tgaccttcaa atttccccat tgttcgataa acgaaaaaac tttatcggac
     7741 gtttggtaat gtggcgtgat atttcacgct tgaagaaaat ccaaaccgag cttgaaaaac
     7801 ttgccacaca agacccgctg actcacgtct acaaccggcg tcatttttac accttggcag
     7861 aagtggaaat tcaccgttct ctccgttctc agttgccctt ctctgtcatt ttggtggaca
     7921 ttgattactt caagagcatt aacgataaat acggacataa agccggcgat gaagcactaa
     7981 tccactttac gaagatctgc atacagaacc tccgcaaagt ggatatattt gccagatttg
     8041 gcggcgaaga atttgtcatc ttgctgcccg aagcggactt ggatcaagcc aaagagacag
     8101 ccgagcgaat tcgaaagatc gtggaaagca cagtgctgaa cattgatgaa catcaaatca
     8161 agatcactgc cagttttggt gtttccacct ttaaaggcag ccccgatacc ttagaccttt
     8221 tgctgcaaaa atcagatgaa gctttgtatg ctgcaaaaaa tacaggacgc aatcgggtcg
     8281 tctgcgcaga ctaacttatg caactaacaa aatcaccaag cctacaatag cggggacagc
     8341 ctgaatgtaa aagatgcgct tgctgac
//
LOCUS       DAOQJE010000024         8191 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_4724_length_8191_cov_8.092797, whole genome shotgun sequence.
ACCESSION   DAOQJE010000024 DAOQJE010000000
VERSION     DAOQJE010000024.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 8191)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8191)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8191
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4724_length_8191_cov_8.092797"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..353
                     /locus_tag="PLT08_01220"
     CDS             <1..353
                     /locus_tag="PLT08_01220"
                     /inference="COORDINATES: protein motif:HMM:NF012722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="histidine kinase dimerization/phospho-acceptor
                     domain-containing protein"
                     /protein_id="HNE03108.1"
                     /translation="TLASQVAVALQNARLYQEQASVVTQLRELDRLKSSFLANMSHEL
                     RTPLNSILGFADVMLEGLDGPLTSTMDNDLRLIQKNGQHLLHLINDVLDMAKIESGRM
                     SLHADTFNVNNLLE"
     gene            399..821
                     /locus_tag="PLT08_01225"
     CDS             399..821
                     /locus_tag="PLT08_01225"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNE03109.1"
                     /translation="MYIEPESDREVEIYADSTRIRQVMINLVNNSIKFTEKGSVALNV
                     SRMDDARVLITVKDNGLGIPTDQLETIFLEFTQVDTTTTRKVGGTGLGLPISRRLVEM
                     HGGRLWAESSGIDGEGSTFFVELPLETRVTEIIEKREK"
     gene            822..2477
                     /locus_tag="PLT08_01230"
     CDS             822..2477
                     /locus_tag="PLT08_01230"
                     /inference="COORDINATES: protein motif:HMM:NF012512.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HNE03110.1"
                     /translation="MAMPLYLECNLCNHQQAYIPFVPAICEKCESQWVEAHYDYESFK
                     REILRGIPNRPNNLWRFQDVLPLKNPSALDLYPAGGTPLWLSQRFAPDLGHGSVYIKD
                     ERYSPTSSFKDRQAAVAVAAMNENGIREAVIASTGNAAVAYAAACARAGIKLWVFMTS
                     RVPQEKLREAALFGAEVIRVSGNYDQTKQIASQFAQRKNLLLDRGASSVPARESMKTI
                     AYEIVEQLGWRAPDWYIQAVSGGLGPLGVYQGFKEMFNMGLIKKVPKLAIIQSAGCAP
                     MVNAFKEGKDVATPVIPDTRITILSTGDPGKAYTYLWNLTQQYGGVMESVTDLEAYEA
                     MKTLAKSEGMAVEPATSVAFAGFQKLSGQGIISKDELVAINCTGHTFPVEKHVLGDQW
                     AVDVHLSKDQSPAPHEGLQAALENLDEKTTTILLVDDNSDDALLIRRLLEGRKNYRMY
                     HAKDGWEGLSVARQKLPDLIVSDLTMPGIDGFGLVEELKLDPRTKDIPVVVVSAKDIT
                     KEERLRLNGQIEALYQKGSLPTRKFVDKVIGVIEDKFEEKGKE"
     gene            2487..2870
                     /locus_tag="PLT08_01235"
     CDS             2487..2870
                     /locus_tag="PLT08_01235"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNE03111.1"
                     /translation="MGNIILCIEDNPDNMMLVKRALESRGYVLLEAFTGNKGVEVAEQ
                     NNVDLILLDINLPDIDGYEVARRLRSSSKHNLAYTPIIAVTANALKGDAEKALAAGCD
                     VYMSKPINIRELWARVEAFVPAPDP"
     gene            complement(2931..4823)
                     /locus_tag="PLT08_01240"
     CDS             complement(2931..4823)
                     /locus_tag="PLT08_01240"
                     /inference="COORDINATES: protein motif:HMM:NF014325.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-binding protein"
                     /protein_id="HNE03112.1"
                     /translation="MKQPTFKQRFQYWFDNYMSKGTPALLFGLFVLSAIIIFAAAALV
                     KLTNTAPNGESLLQIAWMSLLRTLDSGTMGGDTGSPFFLFMMLIVTFGGVFVVSALIG
                     ILNNGLEDKLEELRKGRSVVLENDHTLILGFTPQIFTIISELVAANESRKSGAVIVVL
                     ADDDKVDMEDAIKERIPDTKNTRIICRSGSPMDMTDLEIASPHTARSIIILADGDDPD
                     TRVIKSVLAITNNPNRRSEPYHIVTQIRDPQNMDVIKLLSEKDTVQPILTTDLIARVV
                     AQTSRQSGLSVVYTELMNFGGDEIYFKQEPGLSGKTYGDALLSFETSTVMGIRRADGT
                     IMMNPAMDTRIEPGDSIFAIAEDDDKIQLTSSRLTLEENLIQRSGNRSKPKPERGLIL
                     GWNRSGSIIIRELDNYVAKGSELLVVSDVDGLESQIQREVGKLKNQKLKVMEGDIRDR
                     ALLDELGVTEYDHVIVLAYSHLDVQDADAITLVTLLHLRDIAEKDETPFSIISEMLDL
                     RNRELAEAAKVDDFIVSEHLISLMMAQLSENAELKDVFTDLFDPEGSEVYLKPISEYV
                     TTGEAVTYSTVVEAAKRRGETAIGYRIMSEVHDAEKSYGVRTNPKKSEKVTFASEDKL
                     IVISEN"
     gene            5088..>5509
                     /locus_tag="PLT08_01245"
     CDS             5088..>5509
                     /locus_tag="PLT08_01245"
                     /inference="COORDINATES: protein motif:HMM:NF012401.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HNE03113.1"
                     /translation="MFQFEGFDRRKQEDKVREEGRLPPGQSLTQKFPVLHYGPVPTFN
                     PAAWDFRVWGEVEEEKRWSWDEFNQLPRTKLHMDIHCVTRWSKFDTDWEGVSVKTLVE
                     QGFFKIKPTATHVMQHAEYGFTVNLPLEVVLQENFLLAT"
     assembly_gap    5510..5609
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<5610..6394)
                     /locus_tag="PLT08_01250"
     CDS             complement(<5610..6394)
                     /locus_tag="PLT08_01250"
                     /inference="COORDINATES: protein motif:HMM:NF012318.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HNE03114.1"
                     /translation="MMKYKLVIPVLVLAVLACQTPVFTAQQEPADTTNQSTTSEVIPT
                     LAAPANLDASNPAAAQDVLVALFENVSPGTVAIFTETGQGSGFVYDTQGHIVTNFHVV
                     SQDATTAVDIVEVRFPSGFMAYGSIVGIDLDSDLAVIKVDAPAEELKPVPLGDSSSLK
                     VGQTVVAIGNPFGLQSTMTVGIISALGRTLDSERSSPDGNFFSAGDIIQTDAAINPGN
                     SGGPLFNLNGEVIGINRAIRTFSQNDAGEPVNSGIGFAISINIV"
     gene            complement(6406..7320)
                     /locus_tag="PLT08_01255"
     CDS             complement(6406..7320)
                     /locus_tag="PLT08_01255"
                     /inference="COORDINATES: protein motif:HMM:NF013705.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="J domain-containing protein"
                     /protein_id="HNE03115.1"
                     /translation="MDYKDYYKTLGVDRKASTDEIRTAYRKLAMKYHPDKNPGDKKAE
                     DKFKEINEAYQVLSDEQKRAHYDRLGSAYSNYRSTGGRPGDFRWDDWYQQQAAGQRGS
                     VNMEDLFGGSGGGGFSDFFRTIFGEAMRGQGRGNPFVQDSPGYEQEVSISFQEAFDGT
                     TRQLQKQDGKKLTVRIPAGVKTGSKVRVAGAGPDGSDLYLLIQIEEDGRFERDGSNLT
                     TTSVIDVFTLILGGEADVETPTGKVKLGIPAGTQPEQIFRLAGRGMPSVKDANVKGDL
                     YVKLKVQIPKYLSSKQRELIEEASRIKF"
     gene            7444..8028
                     /locus_tag="PLT08_01260"
     CDS             7444..8028
                     /locus_tag="PLT08_01260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HNE03116.1"
                     /translation="MMKSVCVFCGSSDAVHPSYKKAAFQMGVTLAQNGIQIVYGGGKT
                     GLMGELANGALSVGGAVIGVIITSMNTPALAHTGITRMEVTAEMRSRKARMHELSDGF
                     IAMPGGFGTWDELFETLTWAQTGEHEKPVGLFNVNNFYAPLLAAIDHAVAEGFIFSEH
                     RQAISLETDPAKLIESMRNYEHPHDAVKRWMKAE"
BASE COUNT         2131 a         1985 c         1972 g         2003 t
ORIGIN      
        1 ccacacttgc ctcacaggtc gctgtagcac ttcaaaatgc ccgcttgtat caggaacaag
       61 cttcagttgt aacccagctt cgcgaattag accgcctcaa atcctccttc ctcgcgaaca
      121 tgtctcatga gttgcgaact ccgctcaact ccatccttgg cttcgccgat gtgatgctcg
      181 aagggttaga cggtccgctg acctctacaa tggataacga cctgcgcttg atccaaaaga
      241 atggtcaaca tctgttgcac cttatcaacg atgtgctcga catggcgaag atcgagtctg
      301 gtcgcatgag cttgcatgcc gatacgttca atgtcaacaa cctgctcgaa taagttacca
      361 gcatcacatc atctctggca agcgataaaa acatcgcctt gtatatcgaa ccagaatcag
      421 accgtgaggt cgagatctat gctgatagca ctcgtatccg tcaggtaatg atcaaccttg
      481 tgaacaactc catcaagttc accgagaagg gaagcgtcgc tctgaatgtc tcgcgaatgg
      541 atgatgcccg agttctgatt accgtcaaag ataatgggct tggaattcca accgaccagc
      601 ttgaaaccat cttcttggaa ttcacacagg tcgacactac caccacccgc aaggtcggtg
      661 gaacagggtt gggtctgccg atcagccgcc gccttgtcga aatgcatggt ggccgtctct
      721 gggcagaaag ttccggcatt gatggtgaag gctctacatt ctttgtagaa ttgccccttg
      781 aaactcgcgt tacagaaatc attgagaaaa gagaaaaata aatggcaatg ccgctttacc
      841 tcgaatgcaa tctttgcaac catcaacaag cctacatacc attcgtgcct gccatttgtg
      901 aaaaatgcga atctcaatgg gtcgaagctc attacgacta cgagtcgttc aaacgtgaga
      961 ttctgcgcgg tattcccaac cgcccaaata acttgtggcg ctttcaggat gtcttgccat
     1021 taaagaatcc atctgcactc gacctatacc ccgcaggtgg aactccactc tggctctcac
     1081 agcgcttcgc tccggacctc gggcatggtt ctgtttacat caaagatgaa cgctacagcc
     1141 cgacctcttc tttcaaagat cgtcaagccg cggtggcagt ggccgccatg aatgaaaatg
     1201 gcattcgaga agcggtcatt gcctccacag ggaatgctgc tgtagcctat gcagccgctt
     1261 gtgcacgtgc aggcatcaaa ctatgggttt tcatgaccag ccgtgtgcca caggaaaaat
     1321 tgcgagaagc tgccctcttt ggtgcagaag tgatccgcgt tagcggcaac tacgaccaga
     1381 ccaagcagat cgccagtcag ttcgcgcaac gcaaaaatct gttgctcgac cgcggcgcaa
     1441 gttctgtgcc tgcccgcgaa tcgatgaaga ccattgccta tgagatcgta gaacagctcg
     1501 gctggcgtgc ccccgattgg tatattcaag ccgttagcgg cgggttggga ccgttaggcg
     1561 tttaccaagg tttcaaagaa atgttcaaca tggggctgat caaaaaggtg cctaaacttg
     1621 ccatcatcca gtctgcgggt tgcgctccca tggtcaacgc cttcaaggaa gggaaggatg
     1681 ttgccacgcc agtcattcct gatacacgca tcaccatcct atccacaggc gaccccggta
     1741 aagcatacac ctacttgtgg aacctcactc agcaatacgg cggcgtgatg gagtccgtta
     1801 cagaccttga ggcatacgaa gccatgaaaa ccctcgcaaa aagcgaaggg atggcagtgg
     1861 aacccgcgac ttctgtagca tttgcgggtt ttcaaaagtt atctgggcag ggtatcatca
     1921 gcaaagatga gcttgttgct atcaactgca cagggcatac cttccccgta gaaaaacacg
     1981 tcttgggaga tcaatgggca gtggatgtgc atcttagcaa agaccagtcc cccgctccac
     2041 atgaagggtt gcaagccgcg ctcgaaaact tggatgaaaa gaccacaact atcttattag
     2101 tggacgataa ctcagatgat gcactgctta tccgccgttt gctggaaggg cggaagaact
     2161 accgcatgta tcacgccaaa gatggctggg aaggtctctc cgttgcacga caaaaattgc
     2221 cagacctgat cgtaagcgac ctgaccatgc caggtattga tgggtttggt ttggtagaag
     2281 aattaaagct agacccacgt acaaaagata ttcctgttgt ggtcgttagc gcaaaagata
     2341 taacaaaaga agaacgcctt cgcctcaacg gtcaaattga ggcactatat caaaaaggat
     2401 ccctccccac tcgaaaattc gtggataaag ttatcggggt cattgaagat aaatttgaag
     2461 aaaaaggcaa agaataagga gaacttatgg gaaacatcat cctctgcata gaagataatc
     2521 cggataatat gatgttggtt aaacgcgcac tcgaatcacg cgggtatgtt cttcttgagg
     2581 ctttcacagg aaacaaaggc gtggaagtag cagagcaaaa taatgtcgat ctgattttgt
     2641 tggatattaa cttaccagat attgatggct acgaagtagc aaggcgctta cgctccagca
     2701 gcaaacataa ccttgcgtac actcctatca ttgccgtaac agccaatgcg ttgaaagggg
     2761 atgctgaaaa agcactcgcc gcaggatgcg acgtatacat gtccaaaccg atcaatattc
     2821 gcgaactctg ggcaagggtt gaagcctttg ttccggcgcc cgatccgtaa tttttattca
     2881 acaaaaaaca ccccgagcaa aaagctcggg gtgtttttat tatccgtcta ttagttttca
     2941 gagattacga tcaatttatc ttctgaagca aaggtaacct tctcagattt cttcgggttg
     3001 gtgcgtacgc cataagattt ttcagcatcg tggacttcgc tcatgatgcg atagccaatg
     3061 gcagtttcac cacggcgttt ggcggcttct acaactgtag agtaggtaac tgcttcacct
     3121 gtagttacat attcgctaat aggcttgaga taaacctcag atccttcagg gtcgaaaagg
     3181 tcggtgaaga catctttcag ttcagcattc tcagaaagct gagccatcat caaactgatg
     3241 agatgttcgc tgacgatgaa gtcgtccact ttggcggctt cggcaagttc gcggttgcgc
     3301 aggtcgagca tttcgctgat gatggagaag ggtgtttcat ccttctcggc aatatcgcgc
     3361 aggtgcaaga gagtgacgag agtgatcgca tctgcatctt ggacatcgag atggctatac
     3421 gctaacacga tgacatggtc atattcggtc acgccgagtt catcaagcaa ggcacggtcg
     3481 cgaatgtcac cttccataac cttgagtttc tgattcttga gtttgcctac ttcgcgctga
     3541 atttggcttt cgagtccatc tacatcagac acgaccaaaa gttcagagcc tttggcaaca
     3601 tagttatcca attcgcgaat gatgatggaa ccagaacgat tccagccgag gataagtccg
     3661 cgctcaggct tgggcttgga gcggtttcca gaacgctgaa tcaggttttc ttccagagtg
     3721 aggcgagatg acgtaagttg aatcttgtca tcatcctcag caatcgcgaa gatggagtca
     3781 ccgggttcaa tgcgggtgtc cattgcgggg ttcatcatga tggtgccatc ggcgcggcga
     3841 atacccatca ctgtggaagt ttcaaacgac agaagagcat cgccgtaggt ttttccagag
     3901 agaccgggtt cctgcttgaa ataaatttcg tcgccgccaa agttcatcaa ctcggtgtag
     3961 accacagaca aaccactttg gcgggaagtt tgcgctacga cgcgggcaat gaggtcggtg
     4021 gtgagaattg gctggacggt atctttttca gagagcagtt tgatcacatc catattttgc
     4081 gggtcacgaa tttgggtgac gatgtgatac ggctcactgc gacgattagg gttgttggta
     4141 attgccaaga cagatttgat aacgcgggta tctgggtcat cgccatcggc aaggataata
     4201 atagagcgcg cggtatgcgg gctggcgatc tcaagatcgg tcatatccat tgggctgcca
     4261 gaacggcaaa tgatgcgagt gttcttggta tcagggatgc gttctttgat ggcatcttcc
     4321 atatccactt tatcatcatc tgccaagacg acgataacgg caccactctt gcggctttca
     4381 tttgcggcaa ccaattcgga gatgatggta aaaatttgcg gagtgaaacc aagaatgagg
     4441 gtatggtcat tctcaaggac gacggagcgc cccttgcgaa gttcttcgag tttatcttct
     4501 aacccattgt tgaggatacc aatcagggcg ctgaccacaa acacaccgcc aaacgtgacg
     4561 atgagcatca taaacaggaa gaacgggctg ccagtatcgc cgcccatagt gccggagtcg
     4621 agggtgcgca gtaagctcat ccaggcgatc tggagcaggc tttcaccatt gggggctgtg
     4681 tttgtcagtt tgacaagagc ggcggctgca aaaatgataa tggcagaaag aacaaataag
     4741 ccaaataata acgcgggtgt ccctttggac atgtagttgt cgaaccaata ttgaaatctc
     4801 tgtttgaatg tgggctgctt cataaaacta tctccttgcg cctttgaaaa tttggcttat
     4861 tataagagca attgcatcta tgaccagagg gtattttcat tacatttgat ttacatattt
     4921 tgcagatggt cttaaacgac agaggtcact gagaatttaa gaaaagcatt tcgggcaatt
     4981 caaagctcta gcttcttata caattattag ttttgcaggg taacatctgg cgtttctttc
     5041 atgtctattc catgccagta ggcagacttt agtaaagtga ggtaaccatg ttccaattcg
     5101 aaggttttga ccgccgcaag caggaagata aagtccgtga agaggggcgt cttccccccg
     5161 ggcaatcatt gacccagaaa tttcccgttt tgcactatgg acccgtcccc acatttaacc
     5221 ccgccgcatg ggactttcgt gtatggggcg aagtggagga agaaaaacgc tggagttggg
     5281 atgaattcaa tcaactgcca cgcaccaaac ttcatatgga catccactgt gtgacccgtt
     5341 ggagcaagtt cgataccgat tgggaagggg tttcagtgaa aactctagtg gagcaaggtt
     5401 tcttcaagat caaaccgacc gccacacacg tgatgcaaca tgctgaatat ggctttacgg
     5461 tcaacttgcc cttggaagta gtattgcaag aaaacttcct gctcgccacn nnnnnnnnnn
     5521 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5581 nnnnnnnnnn nnnnnnnnnn nnnnnnnnna cgatgttaat cgagatggca aacccaatgc
     5641 ccgagttgac cggctctccc gcatcattct gactaaatgt acggatcgcg cggttgatac
     5701 caatcacttc cccattcaaa ttgaaaagcg gaccacccga attgccgggg ttgatcgcgg
     5761 catcggtttg aataatatcg ccagcgctga aaaagttccc atctgggctg gagcgttcag
     5821 agtccaaagt acgacctaag gctgagataa tgcccaccgt catggtgctc tgcaagccaa
     5881 acggattacc aatggcaact acggtctgac ccactttgag tgaggatgaa tcgccgagcg
     5941 gcacaggctt gagttcttct gccggtgcat ccactttgat gacggcgagg tctgaatcca
     6001 agtcaatgcc tacaatgctg ccatacgcca taaagccgga aggaaaacga acttcaacaa
     6061 tatccacggc ggtggttgca tcttgcgaaa ccacatggaa gtttgtaaca atatgacctt
     6121 gagtatcata tacaaaacca gagccttgcc cagtttcggt aaagatcgcc actgtgccag
     6181 ggctcacgtt ttcaaacaaa gccaccaaca catcttgtgc agcggctggg ttcgaagcgt
     6241 caagattcgc cggagccgct aacgtgggaa tcacttctga agtcgtggat tggtttgtgg
     6301 tgtctgccgg ttcttgttgt gcagtaaaaa ccggtgtttg gcatgcaagc acagccaaca
     6361 ccaaaaccgg aatcacgagc ttgtatttca tcatggattg aactcctaaa acttgatacg
     6421 ggatgcttct tcgatcagtt cccgttgttt ggacgacaaa tacttcggaa tctgtacctt
     6481 gagctttaca tacaaatcgc ccttcacatt tgcatctttg acactcggca taccacgccc
     6541 tgccaaccta aaaatttgct caggctgtgt gcctgccgga atacccaatt tcactttacc
     6601 ggtcggcgtt tccacatctg cttcgccgcc aaggatcaat gtaaagacat caattacgga
     6661 tgtcgtcgtc aagttgcttc catcacgctc gaagcgacca tcctcttcaa tttggatcaa
     6721 cagataaaga tcgcttccat ctggacccgc acctgccacg cggacctttg aaccagtctt
     6781 caccccagcc ggaatgcgga cggtcaactt cttgccatcc tgcttctgta gctgacgggt
     6841 cgtgccatca aaggcttctt gaaaactgat gctaacttct tgctcatacc ccggtgaatc
     6901 ttgtacgaat ggattcccac gcccttgtcc gcgcatggct tcgccaaaga tggtgcggaa
     6961 gaagtcagaa aatccgccgc cacccgaacc gccgaacaag tcttccatat tgacactacc
     7021 gcgctgaccc gccgcctgtt gttgatacca atcatcccaa cggaaatcac ccggtcgccc
     7081 accagtggaa cgatagttgg agtaggcgct gccaaggcgg tcgtaatgag cgcgtttttg
     7141 ttcatcgctc aaaacctgat aagcttcgtt gatctccttg aacttatctt ccgccttttt
     7201 atcgccaggg tttttgtctg gatgatattt catggcgagt ttacgatatg ccgtgcgaat
     7261 ctcatccgtg ctagctttgc ggtctacacc aagggtttta tagtagtctt tatagtccat
     7321 gacgctattt tcatcccgtc aagggacagg gtcaagcgtc caaaggttgc acttacataa
     7381 ctctaacctg tctgtagtgt ggtagagtca gcgaaaatta ttctaagaag acaaaatgag
     7441 ggcatgatga aatcagtttg tgtattttgt gggtcttcag atgccgttca cccctcctac
     7501 aaaaaggcgg catttcaaat gggcgtgacc cttgcccaga atggcattca aattgtctat
     7561 ggtggcggca agaccgggct gatgggcgaa ctagcgaacg gggctttatc cgtaggtggc
     7621 gctgtgatcg gcgtaattat cacctcaatg aacacccccg cattagcgca taccggcatc
     7681 acccgcatgg aagtaaccgc tgaaatgcga tcacgcaagg ctcgtatgca tgaactatcg
     7741 gatggcttta ttgcaatgcc cggcggtttt ggcacgtggg atgaactctt cgaaaccctc
     7801 acatgggcac aaaccggcga acatgaaaaa ccagttgggc tgttcaatgt gaataacttt
     7861 tacgcgcccc tacttgctgc catagaccat gccgttgcag aaggctttat tttttcagaa
     7921 catcggcagg caatttcgct ggagaccgac cccgctaaac tgatagagtc catgcgaaat
     7981 tacgaacacc cgcatgatgc agtgaaacga tggatgaagg ctgaataagg atcttggatt
     8041 aaacaattta cgcatcaggt aatacgagat ttgtattgcc tgatgcgtaa tgatttaaca
     8101 agcgtgtttt ctatggcaac gtcaaaataa tcggctcgcc tttggtgatg atgacggtat
     8161 gctcaaattg agccgcaatc gtattatcca c
//
LOCUS       DAOQJE010000025         8159 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_4749_length_8159_cov_4.741239, whole genome shotgun sequence.
ACCESSION   DAOQJE010000025 DAOQJE010000000
VERSION     DAOQJE010000025.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 8159)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8159)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8159
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4749_length_8159_cov_4.741239"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(79..1389)
                     /locus_tag="PLT08_01265"
     CDS             complement(79..1389)
                     /locus_tag="PLT08_01265"
                     /inference="COORDINATES: protein motif:HMM:NF013998.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HNE03117.1"
                     /translation="MVKAGRAFIAIMFFVGLIGVLVNGAPIFSRFLYSSVLLALFAWG
                     WTRLIIRSLSFERVTRELRANVGDVFEETFKLSNRSRLPIPWVEIVNRSTLPFAAGSR
                     LFTLVPGKQFRSYMARTWLTRRGRFELGPTRITVGDPFGLFATNKEHKSKDALIVFPM
                     IHEIYSFQTPQGMLTGGQVIRKKSPDITPHAAGVREYVSGDAMKRIHWSTSARRNRLM
                     VKEFEQDPQAEVWLYLDAHQTVHYQKRHEQQEMALHDMLLNKRPKIKLPPSTLEYGVS
                     IAASLAHYFLRQRRSVGFASAGQTFTVLPAERSERQEAKILETLAFVKADGNLSLAGL
                     VATQAAVLPQGSTVILITPTIRSDLLQAVDDLVYHYLSPVIVLLNAKSFEGPSGTAEL
                     VSSLKARRVPVITISCDDDLPQALADISSNFNLQELHAWRTPLS"
     gene            complement(1397..2359)
                     /locus_tag="PLT08_01270"
     CDS             complement(1397..2359)
                     /locus_tag="PLT08_01270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HNE03118.1"
                     /translation="MADVKAFGERVMTSLEKVIVGKRQSLELVVIGLLCQGHILIEDV
                     PGVGKTMLARSLARSLDCIFNRIQFTPDMLPSDVTGVSIYNQQKNEFEFRPGPIIGQV
                     ILADEINRATPKTQAALLEAMEERQVTVDGVTHAMPKPYIVLATQNPIEYEGTFPLPE
                     AQLDRFLLRMRLGYPSAADEIRIMDDQRLQHPLEGLTPVASGDEVLQMSEAVRQVFIS
                     EVVKKYIVDLSTRTRTSQDVYLGASPRGSLFLSRAAQARAALRGRDHVLPDDVKALAM
                     AVLAHRIIVSAAARLRDVSSDRIVQEIVQSTPVPGGSFEATAKG"
     gene            complement(2464..4860)
                     /locus_tag="PLT08_01275"
     CDS             complement(2464..4860)
                     /locus_tag="PLT08_01275"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF001952.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-translocating pyrophosphatase"
                     /protein_id="HNE03119.1"
                     /translation="MFTMGLTSFEQIAIWAVFAVAWLGLGYAIFLRSQILNEDKGTEK
                     MQEVWGAIKDGANAYLQKQFKSILPLIGILFIALFLSVYVVPPSPEATERFSGMSESQ
                     VKLIIGLARALAFVMGAGFSLTVGQIGMRMAIEGNVRVAAASKKSFGDALRIAYRAGT
                     ITGMLTDGLGLLGGTIIFIILGIAAPDALLGFGFGGTLLALFMRVGGGIFTKAADVGA
                     DLVGKVEAGIPEDDPRNPAVIADLVGDNVGDCAGMAADIFESYEVTIVSGLILGLALW
                     HATGRLEWIVFPLLVRGIGVLSSIIGTYFVKAGKTGKSEDAMSAIFSGFLTSAVISAA
                     LFFIAGFLYMGKTMAEYGGWWRMPTAVGVGVLLAILIDRLTEYFTGTHASPVNDIKKS
                     ADTGPATLILNGITVGFESSVWSVLVIALTIIASIFIFGTIPGVEGATRATFILYGVA
                     MTGIGMLTLTGNNVAMDSFGPIADNANGIGEMSWSGKTDKATKDAQQIMADLDAVGNT
                     TKAITKGVAIGSAVIAAVSLFGSFLVDVSRAQSSLGVALADQIQSIGIRVDIPQIFVG
                     MLIGGALPWLFSSFAIQAVTRAASLIVLEVRRQFKLGVLDGKVKPDYAQAVGISTTAA
                     QKELVSLALLGIVTPIVVGLLLGVEALGGFLAGIIVSGQLLAVFLNNAGGAWDNAKKL
                     IEDEPKDPAKNLGKGSERHKAGVVGDTVGDPFKDTAGPALNPMIKVVNLVAVIVAPIV
                     VQYQGLNLGTGVIAAVLLGVLAWAVMRSKAPAQSMTAAVSAPAPAKKAAAPKKKTTKK
                     "
     gene            5053..5463
                     /locus_tag="PLT08_01280"
     CDS             5053..5463
                     /locus_tag="PLT08_01280"
                     /inference="COORDINATES: protein motif:HMM:TIGR00049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly accessory protein"
                     /protein_id="HNE03120.1"
                     /translation="MLQEINTQLFTLTPAASEAVKNILTERNLEGYALRVYVAGGGCC
                     GVNFGMALDNNFRDVDTTFEANGVKVVVDEVSIDYLRDATVDFVNDPMRGAGFAVNSP
                     NAQGHSHGEGGCACGAGGGESSCGCGGGSCSCAN"
     gene            complement(5520..6146)
                     /locus_tag="PLT08_01285"
     CDS             complement(5520..6146)
                     /locus_tag="PLT08_01285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE03121.1"
                     /translation="MFDLRKRLIAFLFIMAFGMWMAFYQPTQNLVVAQQPTGSIPTVT
                     GTPPGMIVRVNIDQNFEIVPVHSGPNSTIYPVVGVILMGQEIPAVGISEDQDWIQIYY
                     PGVPGSTAWVFGPYVTIIKTGRLPVIPAPNTPTPVSTPTINPTLVAAFVDPVTPTRLP
                     TYTPPPEFVVPTFLPDRGSENRVPVGLFIIALGFIGGFGTLISFLRGR"
     gene            complement(6155..6817)
                     /locus_tag="PLT08_01290"
     CDS             complement(6155..6817)
                     /locus_tag="PLT08_01290"
                     /inference="COORDINATES: protein motif:HMM:NF019844.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HNE03122.1"
                     /translation="MNSIIIAFAILLSKLYEMTKYKFHPLVLTLTFLLLVVWIFNASF
                     PTNVHAQQPTDTPAPATGLFVTVVTNEPQINVRMGPSATVYPVIGVLLTGSTVPALGR
                     SKGGDWVQIEFASGPNGKGWVYSPLVEVSPAGSTLIVVDAPPTPVQPATSTIDPTLAA
                     QFIIEPTSTHLPTFTPAPTLIKVQFEPSASLSYSIPWASIIIALAVLGVIGFLLSLVI
                     RR"
     gene            6816..7400
                     /locus_tag="PLT08_01295"
     CDS             6816..7400
                     /locus_tag="PLT08_01295"
                     /inference="COORDINATES: protein motif:HMM:NF024183.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HNE03123.1"
                     /translation="MERKIFHGNIKPVDIAQALLAEFNQGNMRAQILGQSDKMVVQVG
                     TRPDAMSGGQTAMTVTIQTLEEGIMVELGQQAWLGVAASLGATALAAIRNPFSLLGRL
                     DDIAQDIENLQISERVWAVISKAARAAGASAELSENLKRTTCEYCHVANPVGQPSCSA
                     CGAPLGNVQPTTCRHCGFVVRPNEKTCPNCQKPV"
     gene            7410..>8159
                     /locus_tag="PLT08_01300"
     CDS             7410..>8159
                     /locus_tag="PLT08_01300"
                     /inference="COORDINATES: protein motif:HMM:NF023818.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3662 domain-containing protein"
                     /protein_id="HNE03124.1"
                     /translation="MNLREIEARLQTLIEVDLLKVLPGKRVEDVIVLKLATALQQNAI
                     QQDGTTVLPDVYTLILHPETAKAWEDEPFLTVLLNSVATVAQEAGFKFTIPPIITVIR
                     DENIPLNDVEIKTSHRIETMAETNATPLDTGSLEATGEKLPENSFLIVEGVKIYPLNL
                     PVVNIGRRLDNQLVIDDPRVSRNHAQLRAIKGRFVVFDLNSTGGTFVNGQRTSQSVLY
                     PGDVISLAGVALIFGQDNPPPRPDLKDTNPYK"
BASE COUNT         2250 a         2087 c         2004 g         1818 t
ORIGIN      
        1 ttaagacaac agcatcgcct tgtcgtatca gatacgaggc gatggcttga gtcttgcctt
       61 gaaaatggag gtcaatcgtt acgatagggg tgttcgccat gcatgcaatt cctgtaggtt
      121 gaaattagag gatatgtctg ccagcgcctg cggtagatca tcgtcacatg aaatggtgat
      181 gaccggcacg cgccgtgctt tgagtgatga taccagttct gcagttccag atggaccctc
      241 aaatgacttt gcatttaata acacaattac cggagaaaga tagtgatata ccagatcatc
      301 cacggcttgc aaaaggtctg aacgaatggt tggggtgatg agaatcaccg ttgagccttg
      361 cggcaagaca gccgcttgtg ttgccaccaa cccagccaat gaaaggttgc catctgcttt
      421 gacgaatgcc aaggtttcga ggattttcgc ttcctgtctt tcactgcgtt cggcgggcaa
      481 aacagtaaaa gtctgcccgg cgctggcaaa ccctaccgaa cggcgctggc gaaggaagta
      541 atgcgctagt gatgccgcaa tgctcacccc atattccaaa gtagaaggcg gcaatttgat
      601 cttggggcgc ttgttcaaca acatatcatg caacgccatt tcttgctgtt catgccgttt
      661 ctgataatga accgtttggt gcgcatcaag atataaccac acttctgctt gggggtcttg
      721 ctcaaattct ttcaccatca aacggttacg tcgcgcgctt gtagaccaat gaatgcgctt
      781 catggcatcg ccactcacat attcacgtac gcccgccgca tgcggagtaa tatctggcga
      841 tttctttcga atcacctgtc cgccagttaa catgccttgt ggagtctgaa aggaataaat
      901 ctcatggatc atcgggaaaa caatcaaggc atctttggat ttgtgctctt tgttggtcgc
      961 gaacaatcca aacgggtcac ctactgtgat gcgcgttgga cctaattcaa atcgtccacg
     1021 gcgagtgagc caagtccgcg ccatgtagct gcggaattgt ttacccggca ccaaggtaaa
     1081 caaacgtgac cccgctgcaa atggcagcgt ggatctgttc acgatctcca cccatgggat
     1141 gggcaatcga ctgcgattac ttagcttgaa ggtttcttcg aatacatccc ccacattggc
     1201 acgtagttca cgcgtcacac gctcaaagct caaactgcgg ataatgaggc gcgtccaacc
     1261 ccaggcaaag agggcaagca aaacactgct atacaaaaaa cgtgaaaaga tcggtgcccc
     1321 attaaccaga acaccgatca gccctacaaa gaacatgatg gcgataaagg ctcgcccggc
     1381 tttcaccatg cttgggttat ccctttgccg tggcttcgaa cgacccacct ggcaccggcg
     1441 tggactgtac aatctcctga acaatacggt ctgaactcac atcgcgcaac cgggctgccg
     1501 cactgacaat aatacgatgc gccagaactg ccatcgccaa agccttaacg tcatctggta
     1561 gaacatggtc acgcccgcgt aaggcggcgc gggcttgagc cgctcgcgag aggaacaagc
     1621 ttccacgtgg gctggcaccg aggtatacat cctgactagt acgagtacga gtacttaaat
     1681 ccacgatgta ttttttcaca acctcagaaa taaatacctg ccgaaccgct tcactcattt
     1741 gcaaaacttc atcccccgaa gcaacaggcg tcagtccctc gagcggatgt tgcaatctct
     1801 gatcgtccat aatacgaatt tcatccgcag cgcttgggta gccaagcctc atacgaagta
     1861 ggaaacgatc taattgcgct tcagggagcg gaaatgtccc ttcatattca atggggttct
     1921 gagtagccag cacaatatat ggctttggca tggcatgcgt cacgccatcc acagtcacct
     1981 gacgttcttc catcgcttca agcaaagcgg cttgcgtctt cggggtggca cggttaattt
     2041 catccgctaa aatcacctgt ccaataatcg gacccggacg gaattcaaat tcatttttct
     2101 gctggttata aatagaaacg cccgtcacat cactggggag catatcagga gtgaattgga
     2161 tgcgattgaa aatgcaatct aaagaacgcg ccaaactgcg cgctaacatt gtcttcccta
     2221 caccaggaac gtcttctatc agaatgtgcc cctgacataa aagaccgatc actaccaact
     2281 caagagattg tcgtttcccg acgatcacct tttccaaact ggtcatcacg cgctcgccaa
     2341 atgctttcac atccgccatt tgatacgatt cctttcaagg aaagataatt cgattctatc
     2401 acgcgccttc ataaaaaaat cccgccctaa tactacagga cgggattttt ttatgatttt
     2461 ctactacttc ttggtggtct tcttcttggg agcagcagct ttcttcgcag gagccggagc
     2521 agaaaccgca gcggtcatgg actgcgccgg agccttggag cgcatcaccg cccaagccaa
     2581 cacacccaac aacacagcag cgataacacc cgttcccaaa ttcaagccct gatattgaac
     2641 cacgatcgga gccacaatca cagccacgag gttcaccact ttgatcatgg ggttgagggc
     2701 aggaccagcc gtatccttga aaggatcacc gacggtatca cccaccacac cagccttgtg
     2761 gcgttcagaa cccttaccca gattcttggc aggatccttg ggttcgtctt cgatcaactt
     2821 cttggcatta tcccaagcgc caccggcgtt gttaaggaac accgccaaca actgaccaga
     2881 gacaatgata ccggcgagga aaccgccgag ggcttccaca cccagcaaca gaccgacaac
     2941 gatcggggtc acaatgccaa gcaatgcgag agaaaccaat tccttctgag ccgcagtggt
     3001 ggaaattcca accgcctgag cgtaatcagg cttgactttt ccatccaaca cacccagttt
     3061 gaattggcgg cgaacttcca acacgatgag ggaagccgca cgggtcacag cctgaatggc
     3121 gaaggaagag aacagccatg gcaaagcgcc accgatgagc atacccacaa agatctgcgg
     3181 gatatccacg cggataccaa tggactgaat ctggtcagca agggcaacac ccaagctcga
     3241 ctgagcgcgg gacacatcca caaggaagga gccgaagagc gagaccgctg caatcacagc
     3301 agaaccgatg gcaacaccct tggtgatggc tttggtggta ttgcccaccg cgtcaaggtc
     3361 agccatgatc tgctgcgcat ctttcgtcgc cttgtctgtt ttgccagacc aggacatttc
     3421 accaatgccg ttcgcgttat ctgcaatcgg accaaaggag tccatcgcaa cattgttacc
     3481 ggtcagggtc aacataccga taccggtcat ggccacaccg taaagaatga aggtcgcgcg
     3541 ggttgcacct tcaacaccag gaatggtgcc gaaaatgaaa atggaagcaa tgatggtcaa
     3601 agcaatgacc aacaccgacc acacagaaga ttcgaaccca acggtaatac cgttcaggat
     3661 caaagtcgca ggacccgtat ccgcagattt cttgatgtcg ttcacagggg aagcatgagt
     3721 gccggtgaag tattcggtaa ggcggtcgat caaaatcgcg agcaacacac ctacacccac
     3781 agctgtcggc atacgccacc aaccaccgta ttcagccatg gtcttgccca tgtaaaggaa
     3841 gccagcaatg aagaacaaag cagcggagat cacagcagag gtcaaaaagc cgctgaaaat
     3901 agcgctcatg gcatcttcac tcttgccggt cttgccagcc ttcacgaagt aggtaccaat
     3961 gatggaagaa agtacgccga taccacgcac caacaagggg aagacgatcc attcgaggcg
     4021 acccgtagca tgccagagag ccaagccgag gatcaagcca gaaacgatgg tcacttcata
     4081 ggattcgaag atgtcggcag ccataccagc gcagtcacca acattatcac caacaagatc
     4141 cgcgatcaca gcagggttgc gtggatcatc ctcagggata cccgcttcga ctttaccgac
     4201 gaggtcagca ccaacatcgg cagccttggt aaagatacca ccgcccacgc gcatgaacaa
     4261 cgcgaggagc gtgccaccaa agccgaagcc cagcagagca tcgggggctg cgataccaag
     4321 gataataaag atgatggtac cgccgagcag accgagacca tcagtgagca taccggtgat
     4381 ggtgccagca cggtaagcaa tacgaagcgc atcgccaaac gacttcttgg aagccgccgc
     4441 tacacgcaca tttccttcaa tcgccatacg cataccgatc tgacctaccg tcagtgagaa
     4501 gcccgcaccc atgacaaatg ccagagcacg tgcaagaccg atgataagct tgacctggct
     4561 ttcgctcata ccgctgaagc gttcagtcgc ttcaggagat ggcggaacca cgtacacgga
     4621 aaggaacaag gcaatgaaga ggatgccgat caaaggcaag atggatttaa attgcttctg
     4681 caaataggca tttgcgccat ctttaatcgc accccaaact tcttgcattt tttccgttcc
     4741 cttatcctca ttgaggatct gggagcgcaa aaaaatcgcg tatccgagcc caagccatgc
     4801 aacggcaaaa acggcccaaa tcgcgatctg ttcaaaactg gtcaatccca ttgtgaacat
     4861 gaaaggatac tcctccaaca gagatgaaaa aataaaggct gacgcctttt taaaagcaca
     4921 gagattttac agtcgcatag tctttgtgtc aagaaaatga gtttggatta atcttcatac
     4981 aaaaaaagtt gacagcccac tttaaacctg ataacattta ccgaaattag atgtaacaca
     5041 aggagaaacc ttatgctcca agaaattaac acccaacttt ttacacttac cccggctgcc
     5101 agcgaagcgg ttaaaaatat tttgaccgaa cgtaaccttg aaggttatgc tttacgcgta
     5161 tatgttgctg gcggcggttg ctgcggcgtc aactttggta tggcgctcga taataatttt
     5221 cgtgatgtgg ataccacttt cgaagccaat ggcgtcaagg tcgttgttga tgaagtttcc
     5281 atcgactact tacgcgatgc aaccgttgat ttcgtcaacg acccgatgcg tggcgctggt
     5341 ttcgctgtca atagcccgaa cgctcaggga cacagccacg gcgaaggcgg ttgtgcctgc
     5401 ggtgctggtg gcggtgaaag ctcttgcgga tgcggcggcg gttcttgcag ctgcgcaaat
     5461 taatctaaga tcgtaaaatc aaaaggacag gtcatagacc tgtccttttt tgttcctttt
     5521 tatcgaccac gtaaaaatga gatcaaggtg ccaaacccgc caataaaccc gagggcaatg
     5581 atgaataagc caacaggcac acggttttcg gaacctctat ctggcaggaa tgttgggacg
     5641 acgaattctg gcggcggtgt gtaagttggt aaacgcgttg gggtgacggg gtcaacaaaa
     5701 gcagcaacca gagtagggtt aattgtgggt gttgaaacag gcgtcggcgt atttggagcc
     5761 ggaatgactg gtagtcgtcc tgttttgatg atggtgacgt atggtccaaa aacccaggcg
     5821 gttgagccgg gtacgccggg ataataaatt tgaatccagt cttgatcttc agaaatgcct
     5881 acggctggaa tctcttgccc cataagaata accccaacaa cggggtagat cgttgagttc
     5941 ggacctgaat gaacaggtac aatctcgaaa ttctggtcaa tgttcacacg tacgatcatg
     6001 ccaggaggtg taccggttac ggttggaatt gaacctgtgg gctgttgtgc aacgacaaga
     6061 ttttgagttg gctggtagaa cgccatccac atcccaaatg ccattataaa tagaaaggcg
     6121 atcaaacgtt ttctaaggtc gaacatggtc aatttcaacg acggatcacc aaagaaagta
     6181 aaaagccaat cacacccaag accgccaatg caataatgat cgaagcccac ggaatgctat
     6241 agctcaagct ggcagatggt tcaaattgaa ctttgatgag ggttggtgca ggggtaaacg
     6301 tcggcagatg tgtgctggtt ggctcaataa tgaattgcgc ggcaagagtc gggtcaatag
     6361 tggatgtggc aggttggact ggtgttggtg gagcgtctac aacaatgagg gtgctacctg
     6421 ctggtgagac ctcaaccagc ggggaatata cccagccttt tccgttaggt ccggatgcaa
     6481 attcgatctg aacccagtcg ccgccttttg aacgccccaa agctgggact gttgagccgg
     6541 ttaataacac accaatcacg ggatacacgg ttgcgctggg acccatgcga acattgatct
     6601 gcggttcatt ggttacaacg gtaacaaaga gaccggtagc aggggctgga gtatccgtcg
     6661 gttgttgagc atggacatta gtcgggaaag aggcgttgaa tatccaaaca accaataaaa
     6721 gaaaggttaa cgtcagtacg aggggatgaa acttatattt tgtcatttcg taaagtttag
     6781 aaaggagaat cgcaaaggcg attataatcg agttcatgga gcgaaaaatc tttcacggga
     6841 atatcaagcc ggtggatatt gcacaggctt tgctggcaga gttcaatcag gggaatatgc
     6901 gtgcgcagat cttgggtcag agtgacaaga tggttgtgca ggtgggcacg cgcccagatg
     6961 ctatgtctgg cgggcagacc gcgatgactg taacgatcca aacacttgaa gaaggcatca
     7021 tggtggagtt gggtcaacaa gcttggcttg gagtggctgc cagcttgggt gccactgcgc
     7081 ttgccgctat tcgaaatccg ttcagcttat tagggcgctt ggacgatatt gctcaagata
     7141 ttgaaaattt acaaatcagc gaacgtgttt gggcggttat ctccaaggcg gctcgtgcgg
     7201 ctggtgcatc tgccgaactc tctgagaatt tgaaacgcac tacttgtgaa tactgccatg
     7261 ttgccaaccc agttggtcag ccttcctgca gcgcttgtgg agctccgctt gggaacgtgc
     7321 aacccactac gtgccgtcac tgtggttttg ttgtaagacc taatgagaaa acctgcccga
     7381 actgtcagaa acctgtataa ctaaaaacca tgaatttacg agaaatcgaa gcacgtctcc
     7441 aaaccttgat cgaagtagac cttttaaagg ttttgccggg caaacgcgtg gaagatgtga
     7501 ttgtcttaaa actcgcaaca gctcttcaac aaaatgcgat ccagcaagat ggaacgactg
     7561 ttttaccaga tgtctacacg ctcatattgc accctgagac cgcaaaagcc tgggaagatg
     7621 agccatttct tacagttcta ttaaattctg tggcaactgt agcccaagaa gccggattta
     7681 agtttacaat accccctatc attaccgtca taagggatga aaacatccct ttaaacgatg
     7741 tagagatcaa aacatctcac cggatagaaa cgatggcaga aacaaacgca acccccctcg
     7801 atactggcag cttggaagcg actggcgaaa agttacccga aaattcattc ttaattgtag
     7861 aaggcgtgaa gatatatccg ctaaatcttc ccgtggtcaa tattgggcgc cgactcgata
     7921 atcaacttgt gattgatgac ccgcgtgtct ctcgcaacca tgcccaactc cgcgcaatta
     7981 agggacgttt cgttgtattc gacctcaact ccaccggcgg cacctttgtt aacggtcaac
     8041 gcaccagcca aagcgtactc tacccgggcg atgtaatctc actggcaggt gtggctctca
     8101 tttttgggca agacaatccg ccaccgcgcc cagacctcaa agataccaac ccctacaaa
//
LOCUS       DAOQJE010000026         8092 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_4807_length_8092_cov_5.866990, whole genome shotgun sequence.
ACCESSION   DAOQJE010000026 DAOQJE010000000
VERSION     DAOQJE010000026.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 8092)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8092)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8092
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4807_length_8092_cov_5.866990"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..557)
                     /locus_tag="PLT08_01305"
     CDS             complement(<1..557)
                     /locus_tag="PLT08_01305"
                     /inference="COORDINATES: protein motif:HMM:NF015418.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE03125.1"
                     /translation="MLSVTEARERILSHFKINSTETVSLIDAANRILGIDILADGDYP
                     PFDNSAVDGFALHAEDTSASPLTLSVVADIPAGSTPKVTLQAGQAARIMTGAQMPLGA
                     NCVIMVEDTDFNAKSAGTTAPKTVSFSKQMKSGENLRLRGADIHTGDAVLKQGRTLKP
                     QDLGLLATLGHARVTVHKKARVALLS"
     gene            complement(628..>1365)
                     /locus_tag="PLT08_01310"
     CDS             complement(628..>1365)
                     /locus_tag="PLT08_01310"
                     /inference="COORDINATES: protein motif:HMM:NF040246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5913 domain-containing protein"
                     /protein_id="HNE03126.1"
                     /translation="LVKTARAKSKIRLWFKKLEDEQNLATGRTTLERELQRLGIPEVN
                     FEKLARDLGFKIPDEMFIGIGTGDLSMSKVIKQFAEDEEIKDILEANTPAAPQKPTDA
                     VEVLGLKGMLASMAKCCNPMPGDQIVGYITRGRGATIHRLDCPNILQLQDRERLLKVG
                     WGHVEQTFPVPVKIKAYDRQGLMSDISNLLSDENININNVSVNVNRSIADLRLIIEVK
                     DLTQLSRILTRIENLPNVMEAVRNNPG"
     assembly_gap    1366..1465
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1466..2996)
                     /locus_tag="PLT08_01315"
     CDS             complement(<1466..2996)
                     /locus_tag="PLT08_01315"
                     /EC_number="2.7.6.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0015970 - guanosine tetraphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HNE03127.1"
                     /translation="MKSAVVEIPIEKLMEQLPESYTVADRELVMRAYTFAEEAHREQK
                     RNSGEPYINHCIAVASILTELKVPADVIAAGLLHDTVEDTPVTLADIRREFGDVVRLL
                     VDGVTKLSHLPRVSRGGEKAENAESEEEPPVQPALLGRKEDMVPETLRKTFLAMGEDV
                     RVVLIKLADRLHNMRTLGHMPEHKRRRIAAETLDIFAPLANRLGIWQLKWELEDLSFR
                     YVQPEKYKEIVESLAERRPDRERQLEEIKGNLIKLLEKNNIKAEVSGRPKHIYSIFKK
                     MQKKDKSFGMLYDVRAVRLIVPDVPSCYAALGVIHTTWRPVPGEFDDYIAAPKDNHYQ
                     SLHTAVMYDDKRPLEIQIRTQEMHMNAEYGIAAHWRYKEGGKHDKQFEERIKDLRKMM
                     EWGGEVNDATEFVESMRSDVFRDRVYIFTPRGDAIDLPAGSTPIDFAYTVHTDIGHRC
                     RGARVNGKLVPLDTELKTGDQVEILTAKRGGPSRDWLNNSLGLVKTARAKSKIRLWFK
                     KL"
     gene            complement(3120..3572)
                     /locus_tag="PLT08_01320"
     CDS             complement(3120..3572)
                     /locus_tag="PLT08_01320"
                     /inference="COORDINATES: protein motif:HMM:NF012257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HNE03128.1"
                     /translation="MISTEILSQFSLFDGLPESLLKEIAGISNEVSVKKDDFVFREGE
                     KADKLYFLLKGSIVLRVKLTSRPESITVSFVSSPYRSFGWSGVVAPHHYTSSAEADED
                     SSLLIIPAEPFMTLLEKNPEHGFTVMKRIAEVIADRLRNSHQALLKTI"
     gene            3686..4162
                     /locus_tag="PLT08_01325"
     CDS             3686..4162
                     /locus_tag="PLT08_01325"
                     /inference="COORDINATES: protein motif:HMM:NF015999.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA deacylase"
                     /protein_id="HNE03129.1"
                     /translation="MSVTNNVTRFLDSRKIKYIAYELPAEKLGAVEAAQLLNVPQEQV
                     FKSIVTKREKGKPVLTVIPGPRVVDLKLLASFLGEKKMHLPTEREAEQLTGLQAGGIS
                     PLALINKGFQVVIDASAQNFETIYISGGQRGLNIQLGAKDLAGLVNAKFGDVSREG"
     gene            complement(4212..4673)
                     /locus_tag="PLT08_01330"
     CDS             complement(4212..4673)
                     /locus_tag="PLT08_01330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE03130.1"
                     /translation="MRRLVITLFLFLFLVSACGAPSPDDPVSNDTVTVHTPAAQETSN
                     YIPSPADGSLTRGEVSLEAKELASLESYPVQFMLQLKGELPTPCHQLRIATNPPDKDH
                     KVLVDVYSVVNPAEICIQVLVPFEIAFPLGSYPEGKYSLWVNGEMVAEFQA"
     gene            complement(4720..5175)
                     /locus_tag="PLT08_01335"
     CDS             complement(4720..5175)
                     /locus_tag="PLT08_01335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE03131.1"
                     /translation="MRYLNFFLIAVLLSACSTVPTAEPTSTAVPPYRITPDENPYEPK
                     LEDLGRQIDEVTITSVNLAELYDYSPPRTAIGLIGYMPSVCDELRVEISPPDENFNVF
                     IQVYSLVNPDVQCDNVFQQFEARILLGTYSPGRYYVWVNDAPVGDFVSY"
     gene            complement(5265..5942)
                     /locus_tag="PLT08_01340"
     CDS             complement(5265..5942)
                     /locus_tag="PLT08_01340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE03132.1"
                     /translation="MFNLFKKKEPKQSDPFLDMRTQFMGNVNFNEWPKGDSDAQPWSL
                     FVAARKAYSTNNHAEAEKYLRQVTETPDLEPRHYLQAWLFLRHFLKVQPPAEIAKKVY
                     GVMVEVGTPTGVMAVVGYTDHKARTLHSSGGGTVWERPNASMDDKVDALIKAGENAVQ
                     AIPLVVVDIVPAPPKQADHVLICIATPSGIYHGLGTGDFMWKDPNAGPIVNTGGILLQ
                     ALEALKK"
     gene            complement(6021..6716)
                     /locus_tag="PLT08_01345"
     CDS             complement(6021..6716)
                     /locus_tag="PLT08_01345"
                     /inference="COORDINATES: protein motif:HMM:NF013353.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate/glutamate racemase family protein"
                     /protein_id="HNE03133.1"
                     /translation="MKTIGLIGGMSWESSIEYYRIINETAKEKLGGLHSAKSLMVTVD
                     FAEIEKLQHEDRWDEAGQILARCAQDLERGGADFIVLCTNTMHKLADQMTSNVSIPLL
                     HIADATAEKIVSAGIKRIGLLGTRFTMEHDFYKGRLIHNYGLDVLIPEKADRDIIHRV
                     IYEELVLGKIVDASRREYQRIMNNLLAKGAEGIILGCTEIELLITEGDSSAPLFPTTK
                     IHAIAAVEKALAE"
     gene            complement(6713..7153)
                     /locus_tag="PLT08_01350"
     CDS             complement(6713..7153)
                     /locus_tag="PLT08_01350"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF015048.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HNE03134.1"
                     /translation="MRKKVPNHGPCFVCGSENPQSIGITMFVEEDGRLTSEFTLNEAH
                     QGPPGHAHGGASAAILDEVMGLVVWAAGHQVLAANININYRKPLPLHIPLLAEAHITE
                     VRERKIISVGKILLPDSTIAVEGSGVYVIAKHFFENSLLNGVHS"
     gene            7215..7454
                     /locus_tag="PLT08_01355"
     CDS             7215..7454
                     /locus_tag="PLT08_01355"
                     /inference="COORDINATES: protein motif:HMM:NF025807.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoaD/ThiS family protein"
                     /protein_id="HNE03135.1"
                     /translation="MLIRVKMIANYRDALPPGHKHGVIELDVPEGTTVYDAISRFDIP
                     LNDESVIVLNGLTVDMDTPLREGDMVTAFSAIAGG"
     gene            7510..>8092
                     /locus_tag="PLT08_01360"
     CDS             7510..>8092
                     /locus_tag="PLT08_01360"
                     /inference="COORDINATES: protein motif:HMM:NF014750.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016625 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, iron-sulfur
                     protein as acceptor [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde ferredoxin oxidoreductase N-terminal
                     domain-containing protein"
                     /protein_id="HNE03136.1"
                     /translation="MYGWHLRILRVNLTTRKVSTEDVDAQIARDYLGGRGWAIHYMYK
                     EMDPAVDPLSPENMLIFATGPLTATPAPTGNRYMVVTKSPLTGALAHSNSGGEFPTWM
                     KRTGFDMFIFEGKASEPVYVLVNEDQIEIKSAAHVWGKDTHETTDILKAETSDEARVA
                     CIGPAGENLALMAAIMNDKHRAAARSGVGAVMGS"
BASE COUNT         1983 a         1926 c         1944 g         2139 t
ORIGIN      
        1 gatagcaatg ccactcgcgc tttcttgtgg accgttacac gtgcgtgccc aagcgtggcg
       61 agcagaccca agtcttgcgg tttgagagtg cgaccctgtt tcaggacagc atctccagtg
      121 tggatatctg cgcctcgcag acgaaggttc tctcctgatt tcatctgttt ggaaaaggag
      181 actgtcttgg gggcggttgt ccctgcgctt ttcgcgttga aatctgtgtc ttctaccata
      241 atgacacagt ttgcaccgag tggcatttgg gctccggtca tgatgcgtgc ggcttgaccc
      301 gcttgcagag tcactttcgg cgtggatcca gctggaatgt cggcaaccac actcagggtt
      361 aagggagagg cggaggtgtc ttccgcgtgt agggcaaatc catccactgc cgaattgtcg
      421 aacggcggat agtctccatc tgcaagaatg tctattccca agatgcgatt tgccgcatct
      481 ataagagaaa cggtttctgt tgagttgatc ttgaagtggg aaaggatgcg ctcgcgcgct
      541 tcagtaacgg aaagcatatc gaagcgccgc gattataccc ccatctcttt ttcacacctt
      601 atttcctgat gagaaaagcg agaatggtta gcctgggtta ttgcgtacgg cttccatgac
      661 attcggtagg ttttcaatgc gcgtcagaat acgactcagt tgtgtgaggt ctttgacttc
      721 gatgatgaga cgtaaatcgg caatgctgcg gttgacattg acggagacgt tgttgatgtt
      781 gatgttttca tcagaaagca aattggaaat atcgctcatc agaccctgac ggtcatatgc
      841 cttgatcttg actggtacag ggaaggtttg ctcaacatgc ccccagccga ctttgagcaa
      901 gcgttcgcgg tcttgtaatt gaagaatatt cgggcaatca aggcggtgaa tggttgcgcc
      961 gcgtccacga gtgatgtagc ccacgatctg gtcaccgggc atagggttac aacatttcgc
     1021 catagacgcc agcatgcctt tcaagccgag aacttcaacg gcatctgtgg gcttttgtgg
     1081 agcggcaggc gtgttggctt caagaatatc tttgatttct tcatcttcag caaactgttt
     1141 gatgacctta ctcatcgaaa ggtcacctgt accaatccca ataaacattt catctggaat
     1201 tttgaagcca agatcgcgag ctaacttttc aaaattgact tctggaatgc caagcctttg
     1261 taactcgcgt tcaagcgtgg tacgacccgt ggcaagattt tgctcatctt ccagcttctt
     1321 aaaccacaag cggatcttgg atttagcgcg ggcggttttt accaannnnn nnnnnnnnnn
     1381 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1441 nnnnnnnnnn nnnnnnnnnn nnnnnccagc ttcttaaacc acaagcggat cttggattta
     1501 gcgcgggcgg tttttaccaa acccaaactg ttgttcaacc aatcgcggct gggacctccg
     1561 cgtttggcgg tgagaatttc tacttggtcg ccggttttta gctctgtatc caatggcaca
     1621 agtttgccat tcacgcgagc gccacggcag cgatgaccaa tatcggtatg gactgtgtag
     1681 gcaaaatcga tcggggtaga accagccggg aggtcaattg catcaccacg cggagtaaag
     1741 atgtacacgc gatcacggaa cacatcactg cgcatcgatt cgacgaattc tgtcgcatcg
     1801 tttacttctc cgccccattc catcatctta cgcaagtctt tgatgcgttc ttcgaattgc
     1861 ttatcgtgct tgccgccttc tttatatcgc cagtgagccg cgataccgta ctcggcgttc
     1921 atgtgcattt cttgggttcg gatctgaatc tcaagcgggc gcttatcgtc atacatcaca
     1981 gccgtatgca gtgattgata gtgattgtct tttggtgcgg caatgtaatc atcaaactca
     2041 ccgggcacgg ggcgccaagt ggtgtggata acccctaaag cagcataaca agaagggaca
     2101 tctggcacga taaggcgtac agcccgcaca tcatataaca tgccgaagga tttatctttc
     2161 ttctgcatct tcttgaaaat ggaatagatg tgcttagggc gcccactcac ttcggctttg
     2221 atgttgttct tttccaacag cttgattagg ttgcccttga tctcttccaa ttggcgttcg
     2281 cggtctgggc ggcgctcagc gagtgactcg acgatctctt tatatttttc aggctgaaca
     2341 taacgaaaac ttaagtcttc caattcccac tttaattgcc aaatacccag tcgattcgcg
     2401 agcggggcaa aaatgtccaa ggtctctgcg gcaatgcggc gacgtttatg ttctggcatg
     2461 tgacccagcg tacgcatgtt atgcaaacgg tctgccagtt taatcagcac cacgcgcaca
     2521 tcttcgccca tggcaaggaa ggtcttgcgc aaggtttcag gcaccatatc ttctttgcgc
     2581 cctaacaagg caggttgaac gggtggttct tcttcactct cggcgttttc tgctttttca
     2641 ccaccgcgcg aaacacgcgg caaatgagat aacttggtca cgccatcaac aagtaaacgc
     2701 accacatccc caaactcgcg gcgaatatct gcgagtgtta caggtgtatc ttctacggtg
     2761 tcatgtaaca aacccgccgc aataacatca gcaggcactt ttaattcggt caaaatactt
     2821 gccacggcaa tgcagtgatt gatatatggc tcccccgaat tgcgcttctg ttcgcgatgc
     2881 gcttcttctg caaacgtata agctcgcatc acgagttccc ggtctgcaac agtataagac
     2941 tcggggagtt gttccattaa tttttcgata gggatttcta cgactgccga tttcatggat
     3001 gaattttact acgcccgcct ctctctcgga ctttgatatg cctctgtttc ctgatggctt
     3061 tccctccgct gtggcttgat ataaaaacca ctggtttttt caaaccagcg gttcgtttct
     3121 tagatggtct tcaacaatgc ctgatggcta tttcgcaaac gatctgcgat cacttcggca
     3181 atacgcttca taactgtaaa gccatgctca gggttctttt ccagcaaggt catgaagggt
     3241 tctgccggga taataagcag gcttgagtct tcgtctgctt cggcgctgga ggtgtaatga
     3301 tgcggcgcaa ccacaccaga ccacccgaaa cttcggtacg gtgaagagac aaaagacacg
     3361 gtgatgcttt ctgggcgaga ggtcaatttg acgcgcaaga cgatgctgcc cttgagtagg
     3421 aaataaagct tatcggcttt ttctccttca cgaaacacaa aatcatcttt ctttactgaa
     3481 acttcattgc tgatgcccgc aatctctttg agaagagatt ctgggagccc gtcgaacagg
     3541 ctgaactggg aaagaatttc cgtgctgatc atgcaatagc ctccaatctt cagggtgaaa
     3601 tagggaaaat ggggtcgatc agtttcgagt ttagaacgaa gcgcagcacc tttatgttta
     3661 taatattcta caggaaattg tcaatatgtc agtcacaaat aatgtcacaa gatttttgga
     3721 ttccaggaaa attaaataca ttgcttatga gcttccggct gaaaaattag gcgcagtgga
     3781 agcggctcaa cttttgaatg tgccgcagga acaagttttc aagtccatcg ttacaaaacg
     3841 cgagaaaggt aagccggtat tgacggtgat ccccggtccg cgggtggtgg atttgaaatt
     3901 gttggcgagt tttctcggcg aaaagaaaat gcatttgcct accgaacgtg aagccgagca
     3961 gttgaccggg ctgcaagcgg gcggaatctc gccgctggct ttgatcaaca aagggtttca
     4021 ggtggtgatc gatgcctctg ctcaaaattt tgagaccatc tatatttcag gcggtcagcg
     4081 tggactcaat attcagttgg gcgcaaagga tttggcgggg ctggttaacg caaaatttgg
     4141 tgatgtaagt cgagagggat aaataaaaat caaaagagga aagaagctga agcgcttctt
     4201 tcctcttttt gttacgcttg aaattcagct accatttctc cattgaccca taaagaatat
     4261 ttcccttcag ggtaactccc taatggaaaa gcgatctcaa atgggacgag aacttgaatg
     4321 cagatctccg ccggatttac aacagaatac acatctacca aaactttgtg gtctttatcg
     4381 ggcgggttgg ttgcaatgcg taattgatgg cagggtgtgg gcagttcacc ttttaattgc
     4441 aacatgaatt gaacggggta actttccaac gaggcaagct cttttgcttc gagagagact
     4501 tctccacgtg tcaaagagcc gtctgccggg cttgggatgt agttgctggt ttcctgtgcc
     4561 gccggcgtat ggacggtgac tgtatcgttg ctaacggggt catctggcga tggggctcca
     4621 catgcagata caagaaatag gaatagaaac aatgtaataa ccaaacgtct catttttaat
     4681 tctcctgcct gattaacgaa aaaatgtatc gaatggttcc taataggaaa caaaatcacc
     4741 cacaggggca tcatttaccc aaacgtagta cctgccgggg gaatatgtgc caagcagaat
     4801 ccgcgcctca aattgttgaa acacattgtc gcactgcaca tctgggttga ccaagctata
     4861 tacctggata aagacattga agttttcatc cggcgggctg atctccactc gcagttcatc
     4921 gcaaacgctg ggcatgtaac cgatcagccc aatggcggtc cgcggcgggc tatagtcata
     4981 gagctctgca agatttacag aagtaatggt gacttcatca atctgcctgc caaggtcttc
     5041 taatttgggc tcataaggat tttcatcggg tgtgatgcga taagggggaa ctgccgttga
     5101 ggtcggctca gcagtaggga cggtgctaca agcactgagc aagaccgcaa tcaggaagaa
     5161 attaagataa cgcataccgc tgtgattctc cgacgcagat aatatcacgg tcttgtgata
     5221 caaaaaactc actcgggtag ggtttgagtg agttttttgt agggttattt ttttagcgct
     5281 tccaaggctt gcaaaagaat tccgcccgta tttacgatag gacccgcatt cgggtctttc
     5341 cacatgaaat ctccggtgcc aagtccgtgg taaattccgc taggagtggc aatacagatc
     5401 aagacatggt ccgcttgttt gggcggggca gggacaatat ccaccacaac gagcggaatg
     5461 gcttgcacag cattttcacc cgctttgata agggcatcga ccttgtcatc catcgaagcg
     5521 ttgggacgtt cccaaaccgt gccgccacca gaagagtgta aggtgcgcgc cttgtggtct
     5581 gtatatccca caaccgccat cacgcctgtg ggagtgccga cttccaccat gacaccatac
     5641 acttttttgg ctatttcggc agggggttgc accttcaaga agtgacgcaa gaataaccaa
     5701 gcctgcaaat agtgacgtgg ttcaaggtct ggggtttcgg taacttggcg cagatatttt
     5761 tctgcttctg cgtgattgtt tgtcgaatat gcttttcgtg ccgcaacgaa caaagaccaa
     5821 ggctgtgcgt ccgagtcgcc tttgggccat tcattgaaat taacatttcc cataaactgg
     5881 gtacgcatat ccaaaaatgg gtcagactgt ttgggttctt tctttttaaa taagttaaac
     5941 atgatgcctc cagaaatgat cttgctggag tatatgcatt tctcaatgct ggtaaatagg
     6001 acatttggtt ataaaacgct ttattctgcc aacgccttct ctactgccgc aatcgcatgg
     6061 atctttgtgg ttgggaaaag cggtgctgaa ctatcgcctt ctgtaataag caattcgatt
     6121 tcggtgcatc ccaaaataat cccttcggcg ccttttgcca acagattgtt cataatgcgt
     6181 tgatattcac gccgagatgc atctacgatc ttgccaagca caagctcttc ataaattacg
     6241 cggtgaataa tgtcgcggtc tgctttttct ggaatgagca catccaagcc gtaattatgg
     6301 atgagccgac ctttataaaa atcatgctcc attgtaaagc gagtgcctaa tagaccaatc
     6361 cgctttatac ctgccgacac aatcttttca gccgttgcat ctgcaatgtg cagcaacgga
     6421 atactgacat ttgaagtcat ctggtctgct aacttgtgca tggtgttcgt gcatagcact
     6481 ataaaatctg cgccgccacg ctcaaggtct tgcgcgcagc gtgccaaaat ttgcccagct
     6541 tcatcccagc gatcttcgtg ttgcagtttc tcgatctcgg caaaatctac ggttaccatc
     6601 aaactctttg ccgagtgcaa cccgcctaac ttttctttcg ccgtctcatt gatgatgcgg
     6661 taatactcaa tcgacgactc ccaactcatc ccaccaatca agccgatagt tttcatgaat
     6721 gtactccatt caaaagtgaa ttttcaaaaa agtgttttgc gatgacgtac accccgcttc
     6781 cttctacggc aatagttgaa tcaggcaaca gaattttgcc tacgctgatg atttttcttt
     6841 ctcgcacctc ggtgatgtga gcttcagcca gcaaagggat atgaagcgga aggggtttgc
     6901 gataattgat gttgatgttt gctgccagca cttggtgccc agccgcccaa acgaccaagc
     6961 ccattacctc gtctaagatg gctgccgagg ctccgccatg ggcatgcccc ggcggacctt
     7021 gatgtgcctc attcaaggtg aattcagatg ttagtcttcc atcctcttca acaaacatcg
     7081 taattccaat ggattgcggg ttttcactcc cgcatacaaa gcagggacca tggttaggta
     7141 ctttttttct cataacgtca atttaaccac aaagaccttc tgtacccttt ggctgattat
     7201 gctagaatcg ccttatgctg atccgtgtca agatgattgc caactatcgt gacgccctcc
     7261 ctccagggca taaacatggc gtcattgagt tggatgtccc ggaagggaca acggtctacg
     7321 atgctatctc tcggtttgat attcccctta atgatgaaag cgtgatcgtc ttgaacggtc
     7381 tcaccgtgga tatggatacc ccgttaagag agggtgacat ggtcacagca ttttccgcaa
     7441 tcgctggcgg ctaatctcgg ttctaatcgt tgaaacattt caacgttcaa actttttcaa
     7501 ggagttccca tgtacggctg gcacctcaga atcttacgcg taaacctaac tactcgaaaa
     7561 gtaagcactg aagacgtaga cgcacaaatc gcacgcgact atctcggtgg gcgcggatgg
     7621 gcgatccatt atatgtacaa agaaatggat cctgccgtgg acccgctttc gccagaaaac
     7681 atgctcatct ttgccactgg tccgttaacg gctacgcctg ccccgaccgg caaccgatac
     7741 atggtggtaa ccaaatctcc gctgacgggc gcacttgcac actctaactc tggtggtgaa
     7801 ttcccaacgt ggatgaaacg cacagggttc gatatgttta tctttgaggg caaagcctca
     7861 gagccagttt atgttttggt gaacgaagac caaatcgaaa ttaagtccgc ggcgcatgtg
     7921 tggggcaagg atacacacga aacgacagac atcctcaagg cagaaacatc cgatgaagcg
     7981 cgagtcgcat gtatcggacc tgcaggcgag aatcttgcac tgatggcagc tatcatgaac
     8041 gataaacatc gtgcggcggc tcgttcgggt gtgggcgctg tgatgggaag ta
//
LOCUS       DAOQJE010000027         8012 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_4880_length_8012_cov_5.399648, whole genome shotgun sequence.
ACCESSION   DAOQJE010000027 DAOQJE010000000
VERSION     DAOQJE010000027.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 8012)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8012)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8012
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4880_length_8012_cov_5.399648"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..751
                     /locus_tag="PLT08_01365"
     CDS             <1..751
                     /locus_tag="PLT08_01365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE03137.1"
                     /translation="VRNAFVHNLADKALMFRNGPINGKELWEKRKPAPFLLKWEPEFA
                     DIAASGDFGFTTGPWEIQEYRPLAESGGYGYFTSVWKKQTDGTWKVVLDIGTSNPKPA
                     AYTYALQYPPGADKVRSFTPVAKLDAVRDELIQRDAALGRSTAASFSAPSFFEVLSSD
                     ARLQRNEYLPTTHRDTIAARLRGITAWESQPIGGDVAASGDLGYTYGTYAIRRGETSE
                     KGYYVRFWKRNEKNQWKITLDLTDPSPEVKK"
     gene            1305..4100
                     /locus_tag="PLT08_01370"
     CDS             1305..4100
                     /locus_tag="PLT08_01370"
                     /inference="COORDINATES: protein motif:HMM:NF037510.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HNE03138.1"
                     /translation="MKNEKLTRKEIIDNRLKQAGWNVTDRTQVVEEFDIIVDKNLVQE
                     ASTPYAGHQYSDYVLLGKDGKPLAVVEAKKTSVDAAIGREQAKQYCYNIKETQGGELP
                     FCFYTNGHDIFFWDLDNYPPKKVHGFPTRDDLERYLYIRKARKSLAGELINTKIAGRD
                     YQIRSIRAVMEAVEKRKTKFLLVMATGTGKTRTCIALVDALMRAGWAERILFLVDRVA
                     LQNQTLDAFKEFLPNEPRYPKPYEKEIATDRRIYVSTYPSMLNIIRDEKNPLSPHFFD
                     LIVVDESHRSIYNTYQEILDYFNTITLGLTATPTDVIDHNTFQLFECEDGVPTFAYSY
                     EEAVNHIPPFLSNFQVMKIKTKFQNEGISKRTISLEDQQKLIVEGKEIEEINYEGTEI
                     EKKVINRGTNALIVREFMEESIKDTNGVLPGKTIFFCISKAHARRIEEIFDSLYPEYK
                     GELAKVIVSEDPRVHGKGGLLDQFTNNDMPRVGISVDMLDTGIDIREVTNLVFAKPVY
                     SYTKFWQMIGRGTRLLEQEKIKSWCTQKDNFLILDCWDNFEYFKLNPRGKELKGQIPL
                     PVRLFGVRLEKIEEAQTQSNSEIVNKEVQKIRRQAESLPKNSVVIMEAKHELQRLEDE
                     NFWSNLTSDKIEFLEAVVKPLLRTVSDVDFKAMRFEKDIVEVSLAHLAGETEKFEALK
                     ESIIEEIGELPLSINIVAREQELIRQAQSNHYWATINEEKFEKLIQKISPLMKFREAV
                     IPLAPAKFNLKDIVSEKEFVEFGPQHEALSVAKYRELVEKKINELVSSSPILQKLKQG
                     QEITTDEAEQLAEELHNEHPHITIDLLRKVYNHRKAALVQFIKHILGIEQLETFAETV
                     TKAFDDFVSKHSYLTSRQIQFLDLLKNFVLEKGDVSKRNLIESPFTMLHPEGIRGIFN
                     QKEIDEILSLTEKVLAA"
     gene            4101..5669
                     /locus_tag="PLT08_01375"
     CDS             4101..5669
                     /locus_tag="PLT08_01375"
                     /inference="COORDINATES: protein motif:HMM:NF014442.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006306 - DNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-6 DNA methylase"
                     /protein_id="HNE03139.1"
                     /translation="MLQNNAQLKSLIDKLWQNFWEGGIANPLTAIEQITYLIFMKRLD
                     DLEAKRERDAEFTGEKYVSRFAGKFNIPGSNESIDKNKLRWSVFKHKPADEMLMHVQM
                     KVFPFLKELNGETSPFTKHMANAVFIMPKASLLVSAINIVEDIFKEIEKDATEGGHAF
                     QDIQGDVYEMLLSEIATAGKNGQFRTPRHIIKLMAELVAPQLGQRIADPACGTGGFLL
                     GAYQYILTDLVRKKDPAKLGKDEDGFERAAISAVLTQKVKTILDQSFVGYDIDTTMVR
                     LGLMNMMMHGIDEPKIDYKDTLSKGYNEDSRFDIVLANPPFTGNIDKGDINEGLKIPT
                     TKTELLFVERIFNMLKMGGTAAVIVPSGVIQNSGKAFEAVRKLIIEKAELKAVIAVPS
                     GAFKPYAGVSTAILIFTKGGETNHVWFYDMQADGYTLDDKRNKIAESDLPDIVQRYKT
                     RDAKKDSDRKLKYFMVPKKEIVENNYDLNLSTYKEEVYEEVSYEKPNVIFSKLETLEA
                     DIQKGLAELKEIVW"
     gene            5768..6202
                     /gene="tnpA"
                     /locus_tag="PLT08_01380"
     CDS             5768..6202
                     /gene="tnpA"
                     /locus_tag="PLT08_01380"
                     /inference="COORDINATES: protein motif:HMM:NF013921.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
                     /protein_id="HNE03140.1"
                     /translation="MYKSFFAVKCRENLIAKSWKDELHKYISGIIKEKEQKPIIVNGM
                     PDHIHAFIGLKPSMRISDLVRDIKNNSSKFINEKGFVRGKFSWQEGYGVFSYSHSHIE
                     RVYNYILNQEEHHRKKTFKDEYLGLLRKFEIDFKEEYVFEFI"
     gene            6338..7465
                     /locus_tag="PLT08_01385"
     CDS             6338..7465
                     /locus_tag="PLT08_01385"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF013579.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HNE03141.1"
                     /translation="MMFEKLGDIATIKTGKYDVNHSTPDGEYVFYTCAFGQFKSPTYS
                     FEGPAIILPGNGANVGEVFYYDGKFEAYQRTYVVQNFKADPNYIYAYFKAQWKNSLRN
                     KQFGSATNYIRLNNLTDFKIPLPPLPDQLHIANLLSKAENLIAQRKESIRLLDEFLKS
                     TFLEMFGDPSSNRKQFPKGSIRDVVAEVKYGTSSPAEDEGLYPYLRMNNITPDGYMDY
                     SKLKYINIVDEKEKEKYVVRKGDLLFNRTNSKELVGKTGVFNEDSEMIIAGYLIRVRT
                     NEKANPWFLWGYLNSIHGKQTLLGMCKSIVGMANINAQELQGIKILIPQIELQTQFAQ
                     IVKKTEALKTQYQQSLQELENLYGSLSRKAFRGELGVKEMA"
     gene            7676..>8012
                     /locus_tag="PLT08_01390"
     CDS             7676..>8012
                     /locus_tag="PLT08_01390"
                     /inference="COORDINATES: protein motif:HMM:NF012792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNE03142.1"
                     /translation="MPIEYSFDKRPSADQIIALYDRSGLPRPTQDKERIQKMYDHSDL
                     IVTAWDGDLLVGVSRSITDWVWSCYLADLAIHPDYQKNGIGKELVRLTKEKVGEEVMI
                     LLLSVPTAMT"
BASE COUNT         2638 a         1632 c         1710 g         2032 t
ORIGIN      
        1 cgtgcgcaat gccttcgttc acaatcttgc cgataaagca ttgatgtttc gcaatggccc
       61 catcaatggc aaagaattat gggaaaagcg caaacccgcg ccgtttttac tcaaatggga
      121 accggagttt gccgacatcg ccgcctccgg tgattttggt tttacgacag gtccttggga
      181 gatacaggaa tatcgcccgc tcgcggagtc cggcggctac ggttacttca catcggtatg
      241 gaaaaaacaa accgacggca catggaaagt cgtactcgac atcggcacat ccaatccgaa
      301 accggcagca tacacctacg ctttgcagta cccgccgggc gccgataaag tccgatcgtt
      361 tacccccgtc gctaaacttg atgccgttcg ggacgaactg atccagcgcg acgccgcgct
      421 gggccgctcg acggcagcgt cattctcggc cccgtcgttt ttcgaagtgc tatcaagcga
      481 tgcacgtttg cagcgcaatg aatacctgcc cacgacccat cgcgatacga tcgcagcacg
      541 tttgcgcggg atcaccgctt gggaatccca acccatcggc ggcgatgtag ccgcttccgg
      601 cgacctcggc tacacgtacg ggacgtacgc catccggcgc ggtgagactt ccgaaaaggg
      661 ctactacgtg cgcttctgga aacgtaacga aaaaaaccaa tggaaaatca cactggatct
      721 caccgatcct tcaccggaag tcaaaaaata aaactgtcgc tgatttttta tcttggcgtc
      781 ttcgcgagaa cgtatcccac atcccggtca ttgcgtaggt ttgctgtacc gaaatgacca
      841 ggaaaccaat ccctcgcaaa gtcgctagga gaacctgaaa aatattctta ataaacctct
      901 tgtaccttgg cgtctctgcg agaacgtatt ctatcccggt tactgtgtag acttgcctta
      961 tcgaaatgac caggaaaccc aatcctcgct cagtcacatc cgaaagaatt gattctgtat
     1021 aatagttaaa atcatcatgc acttccgtgt gtaacgcctg ctcgtatcca tcgattagta
     1081 acgtctcggc gagaatcatt ttttttcaac acatccatcg tatgccgtga atgcgatatt
     1141 cccgcacagc cttgtacgta ttaactcttg cataaaacct gtgataggcg catctttata
     1201 ttaatctcta aaactccaaa aaagatttaa tgtttttcgc atggcaatgt acaacgaata
     1261 taaatcactg cgttagaatt aaacctatta attttcattt ttaagtgaaa aacgaaaaac
     1321 tgacaaggaa agaaatcatt gacaaccgtt tgaaacaagc gggatggaat gtgactgacc
     1381 gaactcaggt agttgaggag tttgacatca ttgttgacaa gaaccttgtt caagaggcct
     1441 cgacaccata tgcagggcac caatatagcg actatgtatt attaggcaaa gacggaaaac
     1501 cgcttgcagt tgtagaagcc aaaaagactt cggtagacgc tgcaattgga agagaacaag
     1561 cgaaacaata ttgttacaat attaaggaaa cacagggtgg agaacttccc ttctgttttt
     1621 acaccaacgg gcacgatatt ttcttttggg atttagataa ctatccgcca aagaaagttc
     1681 atggcttccc gacaagagac gaccttgaac gctacctata catacgcaag gcacgaaaga
     1741 gtttagctgg tgaactcatc aatacaaaaa ttgcagggag ggattatcaa atccgttcca
     1801 ttcgtgccgt gatggaagcc gttgagaaac ggaaaacaaa attccttctg gtaatggcaa
     1861 ctggcacggg taaaacacga acctgtattg ccttggtgga tgcactaatg agagcaggct
     1921 gggctgaacg tattttattt ttagttgaca gagttgcttt gcaaaatcaa acacttgatg
     1981 cattcaaaga attcctaccc aacgaaccac gataccctaa accatacgag aaagaaattg
     2041 caaccgacag acgaatttat gtttcaacat acccaagtat gttaaatatt attcgtgatg
     2101 aaaaaaatcc tttaagtcct cacttctttg atttgattgt tgtggatgaa agccaccgca
     2161 gtatttacaa tacttatcaa gaaattctag actatttcaa cacaattaca ttgggtttga
     2221 cagcaacacc aactgatgta attgaccaca acacttttca gctatttgag tgcgaagatg
     2281 gcgttccaac ttttgcatat tcctacgaag aagcggtaaa tcacattccg ccattcctga
     2341 gtaattttca ggtaatgaaa attaaaacga agtttcagaa tgaaggaata agcaagcgaa
     2401 caatttcttt ggaagaccaa cagaaactaa ttgttgaagg aaaagaaatt gaagaaataa
     2461 actacgaggg aacagagatt gaaaagaaag ttatcaatcg aggcacaaac gctttgattg
     2521 ttcgggagtt catggaggaa tcaatcaaag atacaaacgg ggtattaccc ggaaagacaa
     2581 tcttcttttg tatcagcaaa gcacatgcaa gacgaataga ggaaatcttt gattcacttt
     2641 accccgaata caaaggcgaa ttagcgaaag ttattgtaag tgaagaccca agagttcacg
     2701 gcaaaggcgg attacttgac cagttcacaa ataatgacat gccacgtgta ggtataagcg
     2761 tcgacatgct tgacacaggc attgacatcc gtgaagtaac caatcttgtt tttgcaaaac
     2821 cagtttacag ttacaccaag ttttggcaaa tgattggacg tggcacacga ttattggaac
     2881 aagaaaaaat aaaatcttgg tgcacacaaa aagataattt tctgattctt gattgttggg
     2941 acaactttga atatttcaaa ctcaatccga gaggtaaaga actgaaaggt caaatccctt
     3001 tgcctgttcg cttgtttggt gtgcgattgg aaaagattga agaagcccaa actcaaagca
     3061 attccgaaat tgtaaataaa gaagttcaga aaatcagaag gcaggccgaa tcactgccta
     3121 aaaactcggt agtgattatg gaagccaaac acgaactgca acgcttagaa gatgaaaact
     3181 tctggagcaa cctgacttct gataaaatag aatttttaga agccgttgta aaaccattgc
     3241 ttcgcacggt ttcagatgtt gatttcaaag ccatgcgttt tgaaaaggac attgtggaag
     3301 tttcattggc tcatttggct ggtgaaactg aaaaatttga agcgctgaaa gaaagcatca
     3361 ttgaagaaat tggcgaactg cctttgagca tcaacattgt tgcaagagag caggaattga
     3421 ttcgtcaagc tcaaagcaat cattactggg caaccattaa tgaagaaaag tttgaaaaac
     3481 tgattcagaa aatttcacct ttgatgaaat tccgtgaagc ggtaattcca ttagctcctg
     3541 caaagttcaa tctgaaagac attgtttccg aaaaggaatt tgtagagttt ggtcctcaac
     3601 acgaagcgtt gagtgtagcc aaataccgtg aattggttga gaagaaaatc aatgaattgg
     3661 tttcaagcag cccgatttta caaaaattaa aacaaggaca agaaatcaca acggatgaag
     3721 ccgagcaatt ggcagaagaa ctgcacaacg agcatccaca tattaccatt gatttgctcc
     3781 gtaaggttta caatcaccgc aaagccgcat tggtgcaatt cattaaacac attttaggca
     3841 ttgaacagtt ggaaaccttt gccgaaactg ttaccaaagc atttgatgat tttgtttcca
     3901 aacacagtta cctcaccagc cgtcaaattc aatttcttga tttgctcaag aattttgtat
     3961 tggaaaaagg tgatgtaagc aaacgcaacc tcattgaatc tccttttacc atgcttcacc
     4021 ccgaaggcat cagaggcatt ttcaatcaaa aagagattga cgaaatttta agtttaaccg
     4081 aaaaagtatt agcagcatag atgttacaaa acaacgcaca attaaaatcg cttatagaca
     4141 aactctggca aaacttttgg gaaggcggca ttgccaaccc cttaaccgct attgagcaaa
     4201 ttacctacct cattttcatg aagcgtttgg atgatttgga agccaaacgg gaacgggatg
     4261 cggagtttac aggcgaaaaa tatgtttccc gatttgccgg caaattcaat attccgggaa
     4321 gtaatgaaag cattgataaa aacaaattgc gttggagtgt ttttaaacac aagcccgcag
     4381 acgaaatgct gatgcatgtg caaatgaaag tgtttccatt tttgaaagaa ctaaacggtg
     4441 aaacatcacc tttcaccaaa cacatggcca acgctgtgtt cattatgccc aaagccagtt
     4501 tgctggtttc ggccatcaac attgtggagg acattttcaa agaaattgaa aaagatgcca
     4561 ccgaaggcgg gcatgctttt caagacatac aaggtgatgt ttatgaaatg ctgttgagtg
     4621 aaattgccac cgcaggtaaa aatggacagt tccgcactcc ccgacacatt attaaactga
     4681 tggctgaatt ggtggctccg caattgggac aacgcattgc cgaccctgct tgtggaacag
     4741 gcggtttttt gttgggtgct tatcaataca ttcttaccga tttagttcgc aagaaagacc
     4801 ctgccaaact gggcaaagac gaagacggtt ttgaacgtgc cgccatcagc gccgtactta
     4861 cccaaaaagt aaaaaccatt ttagaccaga gttttgtggg ttatgatatt gacaccacga
     4921 tggtgcgttt gggtttgatg aatatgatga tgcacggcat tgatgaacct aaaattgact
     4981 acaaagacac gctcagcaaa ggctacaatg aagacagccg gtttgatatt gtattagcca
     5041 atccgccctt tacaggaaat attgacaaag gcgatattaa cgaaggtctt aaaataccca
     5101 ccaccaaaac cgaactgcta tttgtagaac gcatttttaa catgctgaaa atgggcggaa
     5161 cggctgccgt aattgtgccc agcggtgtga tacaaaacag cggtaaagcc tttgaagcgg
     5221 tgcggaaact gattatcgaa aaagccgaac tgaaagccgt gatagctgtg ccaagcggag
     5281 catttaaacc ctatgcaggt gtaagcacgg caattctcat ttttaccaaa ggtggtgaaa
     5341 ccaaccatgt atggttttac gatatgcagg cagacggcta cacattggat gataagcgaa
     5401 acaaaattgc cgaaagcgat ttgcccgaca ttgtgcaacg ctacaaaaca agagacgcca
     5461 aaaaagacag tgaccgaaag ttgaaatact ttatggtgcc gaaaaaggaa attgtagaga
     5521 acaactacga tctgaacctg agtacctaca aagaagaagt gtatgaagaa gtgagttatg
     5581 aaaaaccgaa tgtgattttt agcaaattgg aaactttgga agccgatatt caaaaaggat
     5641 tagcggaatt aaaagagatt gtgtggtaaa atcccgaagg gatgaaagga taataaaaaa
     5701 aatgataaac aataaaatca aaaccccgaa ggggtgacat acatggcggg aacctattca
     5761 caaatatatt tacaagtcgt tttttgctgt aaaatgcaga gaaaacctca tcgccaaatc
     5821 atggaaagac gagttgcata agtacatttc aggcattatc aaagaaaagg aacaaaaacc
     5881 aattatcgta aacggaatgc ccgaccacat tcatgcattc ataggtttaa agccatcaat
     5941 gagaatttcg gatttggtac gtgacataaa aaacaattca tcaaaattca ttaacgaaaa
     6001 aggctttgta aggggcaagt tttcctggca ggaaggttat ggtgtgtttt cctattccca
     6061 ttcgcatatt gaaagggtgt ataactacat actgaatcag gaagaacacc accgtaaaaa
     6121 gacgttcaaa gatgaatatc tgggattgtt aagaaagttt gaaattgatt tcaaagagga
     6181 gtatgtattt gagtttatct aaatatatca ccccttcggg gttgggtagt tgggttagcc
     6241 tggtgtgttt caatcatgtc aacccttcgg gtttattatc aatatgcatg gaagccgata
     6301 ttcaaaaagg attggcggaa ttaaaagagt ttgtgtgatg atgtttgaga aacttggaga
     6361 tattgctaca attaaaacgg gcaagtatga tgtaaaccat tctactcctg atggagaata
     6421 cgtattctat acttgtgcat ttggccaatt caaatcaccg acttactctt ttgaaggccc
     6481 tgcaattata ttacccggga atggggcaaa tgttggtgaa gttttctatt acgatggaaa
     6541 atttgaagca tatcaaagga cttatgtggt tcaaaatttc aaagctgacc ccaattacat
     6601 ctatgcctat tttaaagcac agtggaaaaa cagtttaaga aataagcagt ttggttctgc
     6661 tacaaactac attagactaa ataatcttac ggatttcaaa attccccttc ccccacttcc
     6721 cgaccaactc cacatcgcca atctcttaag caaagccgaa aacctgatag cccaacgcaa
     6781 agaaagcatc cgcctgctgg atgaattttt gaaaagcacg tttttggaga tgtttgggga
     6841 tccttcttcc aacagaaagc aatttccaaa aggctcaata agggatgttg ttgccgaagt
     6901 aaaatatgga acaagtagtc ctgcggaaga cgaaggatta tatccgtatc taaggatgaa
     6961 caatataact cctgatggtt atatggatta ttcaaagctg aaatacatca acattgttga
     7021 tgaaaaggag aaagaaaaat atgttgtgag aaaaggagat ttacttttca atcgaacaaa
     7081 ttctaaagag ttagttggaa aaactggtgt cttcaatgaa gattcagaaa tgattatcgc
     7141 tggttattta attcgtgtta ggactaatga aaaagcaaat ccttggttcc tatggggcta
     7201 tttgaattca attcatggca aacaaacact acttggaatg tgtaaaagca ttgtcggtat
     7261 ggcgaacatt aatgcacaag aattacaagg aataaagatt ctgattcccc aaattgaact
     7321 ccaaacccaa tttgcccaaa tcgttaaaaa aaccgaagcc ctcaaaaccc aataccaaca
     7381 gagtttgcag gagttagaaa atttgtatgg cagtttaagc cgaaaggcgt ttagggggga
     7441 gttgggtgta aaggaaatgg cataataaaa aatacgccac cgcacagact aacacaacaa
     7501 agacaatggt ttgcaatggt tgaccgccag aaggtctact gataacatgc gtttggcgtc
     7561 acgcgaagtg aagtgcttaa attcaagttc aatttttcaa atcaacttta gcgctggttg
     7621 atcccgcacc aataccaaac tgagaatcct taaccttaac tatcaaacca ccgccatgcc
     7681 catcgaatat agttttgaca aaagaccctc cgctgaccag atcatagcgc tctatgatag
     7741 atcgggtctg ccgagaccga cccaggacaa agaacgcata caaaagatgt atgaccattc
     7801 cgatctgatc gtaaccgcct gggacggcga cctgctggtc ggggtttccc gctcgatcac
     7861 ggattgggtt tggagttgtt acctcgccga cctggcgatc catcccgatt accaaaaaaa
     7921 cggcatcgga aaggaactgg ttcgcctcac caaagaaaaa gtgggcgaag aagtcatgat
     7981 acttttgctt tcggttccca ccgcgatgac ct
//
LOCUS       DAOQJE010000028         8008 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_4885_length_8008_cov_6.251980, whole genome shotgun sequence.
ACCESSION   DAOQJE010000028 DAOQJE010000000
VERSION     DAOQJE010000028.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 8008)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8008)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8008
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4885_length_8008_cov_6.251980"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..998)
                     /gene="tyrS"
                     /locus_tag="PLT08_01395"
     CDS             complement(<1..998)
                     /gene="tyrS"
                     /locus_tag="PLT08_01395"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HNE03143.1"
                     /translation="MNIEEQVELLMQGTDYGDEDLQKAMASELRERLKIAEKENRPLR
                     VYCGYDPTSTDLHLGHTISMRKLRQFQDLGHEVTFLIGNYTALVGDPSDKNKARPILT
                     QEQVAENAKTYAEQAFRVLDKSKTKIRYNSEWLSKLSLVDLIRLGQNFTVQQFLAREN
                     FAKRLDKGEPIYLHETFYALMQGYDAAAMETDIQVGGSDQLFNIIVAGRKLQEALGQK
                     PLVGIITGILPGTDGAQRMSKSTGNIVPINTGADDMFGKLMSVPDSAMGTYMRLVTRF
                     NPHEIAQIEKDVASGALHPRDAKMKMAQEIVSIFYTDAEAVSAQDHFIKMFQQKEVP"
     gene            complement(1193..1873)
                     /locus_tag="PLT08_01400"
     CDS             complement(1193..1873)
                     /locus_tag="PLT08_01400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE03144.1"
                     /translation="MKKMSIALLLMILISACAPAQDSQVVLPQLLPTATAYIDASSYP
                     TAEANVSAAQQTAAGIDVRMNRAWMEGKNVNAEVCFTLPDASDWSIWAANLAYSGIVL
                     QEYGTTLQSIQESANGQPGVRCDILTFVVAPDADLTNAAINIDAIAAPPQPSEYCDLY
                     LPKIQQAMLERGTGVSVDCVDVNGALTMQILNKPAEMSQEQAEQLVYSDEFYTVRGPW
                     SFPFNLSQ"
     gene            complement(1896..>2351)
                     /locus_tag="PLT08_01405"
     CDS             complement(1896..>2351)
                     /locus_tag="PLT08_01405"
                     /inference="COORDINATES: protein motif:HMM:NF019938.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1731 domain-containing protein"
                     /protein_id="HNE03145.1"
                     /translation="TQSVEGLGVRRLVLRTPPVLSRDNVIMKLIALPVQLFVGGPIGS
                     GKQPFPWIHIKDWVSAVRYLMADESARGVYNMIAPSQTSLADFTKILANVLHRPYWFP
                     VPAFLMRNVLGEMSVLILEGRYSQPKRLLESGYTFQFPGPREALTDLFG"
     assembly_gap    2352..2451
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            2631..3134
                     /locus_tag="PLT08_01410"
     CDS             2631..3134
                     /locus_tag="PLT08_01410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HNE03146.1"
                     /translation="MNEPVLVLNANFEPIHVCSTRRAVTLILAGKAGMIANGRGHIRT
                     ISQLIPRPSVIRLESQIHRPRPRVKLTRREIFRRDNYTCQYCGKRDTNLTIDHVIPRH
                     MGGKQVWTNLVTACSPCNHRKGGRKVDEAHMHLLHIPKEPPADASYIFGKHLAENQEW
                     TPYITGW"
     gene            complement(3168..3923)
                     /locus_tag="PLT08_01415"
     CDS             complement(3168..3923)
                     /locus_tag="PLT08_01415"
                     /inference="COORDINATES: protein motif:HMM:NF024118.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HNE03147.1"
                     /translation="MAKVIILGASNAIPTSESENTHMVIVGNDRTVLIDSVSNPILRL
                     EQAGLDFNNLTDIIITHFHPDHASGIPLLLMDMWLMGRQKPLNIYGLHYTLDRVEGLM
                     GFYNWSEWPNFFPVVFYRLPVREMTPVLDCPDFIIHASPVHHMIPNIGLRVNFQQSQK
                     VMAYSCDTEPCDEVVRLSEGADVLIHEATGESPGHSSATQAGEIASRAEVNKLYLIHY
                     PTGRFKKGDIAAEASQTYQGEIQIAKDFMVLDF"
     gene            complement(4025..5458)
                     /locus_tag="PLT08_01420"
     CDS             complement(4025..5458)
                     /locus_tag="PLT08_01420"
                     /inference="COORDINATES: protein motif:HMM:NF024091.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage II sporulation protein M"
                     /protein_id="HNE03148.1"
                     /translation="MFNKLRPVWLLAQRELRDQFRDWRVLIPMAILTFCFPFLMNEFA
                     KQTVDFLNQYNANLILDRLVPFSIMIIGFFPITVSLVVALESFVGEKERGTIEPLLTA
                     PFDDWQLYFGKLLVGAATPLIASYVSIGLYMLLIIRQDLTMPSASVMVQLLLLTGAHA
                     VLMVSAAIVISVQSTSVKAANLLASFIVIPVAILMQGEAVMLFWGNETVLWLGVAGVL
                     IISLLLIRVGIAHFEREYLLGREIDNLNFKWMLRTFSKNFTGGAASVGDWYRRVVASS
                     FRRALPAMGWMLLVAVVSVWMSYNWVTTNVSQAITSASPDTLAKLEKGIENLPDLSSL
                     GGQLSAPFLFFNNTRSMTVIFLAGIFSFSVLGVLLYMVNIALIGGVYAVLELLGIAPA
                     PIFLAGILPHGIFELPALLIGSAVVLYIGAALVTPQNGKSMGEVMIDLMADWAKIYVG
                     VVVPLLAIAALIEAYITPSILLRVVGN"
     gene            complement(5448..6383)
                     /locus_tag="PLT08_01425"
     CDS             complement(5448..6383)
                     /locus_tag="PLT08_01425"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNE03149.1"
                     /translation="MIETNDLSKQFHDFMAVDGVNLSIGAGQILALLGQNGAGKTTTV
                     RMLTALLQPSRGSARVAGYDVTKNGHDVRASVGVLTEHHGLYMRMTGSEYMDFFGQVY
                     NLDPSLRKSRTENLLEYFGLAEASKRRIGEYSKGMRQKLALARAMMHEPSVLLLDEPT
                     SAMDPESARLVRDEIARLKSTQRTIMLCSHNLTEVELLADKIAIIYRGKILIQGSLDE
                     LKSGVLGAPEYVIKFASAWDASRLEMPKGMELLEKTATSLKVRVEAPAESNPELIQRL
                     TSLSAPVVAFQEETRTLEQVYLKVMATAKGKEYVQ"
     assembly_gap    6620..6719
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<6720..7018)
                     /locus_tag="PLT08_01430"
     CDS             complement(<6720..7018)
                     /locus_tag="PLT08_01430"
                     /EC_number="2.4.99.-"
                     /inference="COORDINATES: protein motif:HMM:NF013915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HNE03150.1"
                     /translation="MTDGLNLGFLLIRWNGLLIALGIAVGALVAAIEAKRRFQDAELI
                     YYLFVPVTVWSWIGARLWHVFTPPLSSVQLGLTTSHYLSHPLDILSVWTGGFGIPG"
     gene            complement(7015..7644)
                     /locus_tag="PLT08_01435"
     CDS             complement(7015..7644)
                     /locus_tag="PLT08_01435"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:NF013250.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HNE03151.1"
                     /translation="MNTKPTFSDRARIIFKGILDPIGNFLNGLGLTPNTITLLGLAGT
                     TVGAYVISQGHMTTGAFILLASVLVDAFDGTMARLRGEPSDFGGFVDSVSDRYAELVT
                     FGGLVYYFLTKADYPGVMISFFAAAGSVLVSYVKARAEGLGFTAKVGILTRLERYIVL
                     IPLLVFNQPFAAVIIIAVLANITAFQRILNVRAQGRERIKKSKESKEAK"
     gene            complement(7657..>8008)
                     /locus_tag="PLT08_01440"
     CDS             complement(7657..>8008)
                     /locus_tag="PLT08_01440"
                     /inference="COORDINATES: protein motif:HMM:NF020952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HNE03152.1"
                     /translation="AQGVLITDTEIERIIAYWQKNWDTDAVEVPPWETLLKEPEEDED
                     DGLVEKAISIVRQQQRASASMLQRRLRIGYPRAARLLDQLETMGVVGPSQGGGKEREV
                     LISDEDEIGNQEER"
BASE COUNT         2192 a         1930 c         1764 g         1922 t
ORIGIN      
        1 gggacttcct tttgttggaa catcttgatg aaatggtctt gagccgatac agcttcagca
       61 tccgtataga agatgctgac aatttcttgt gccatcttca tcttggcatc acgcgggtgt
      121 aatgcacctg aagcaacatc tttttcgatc tgggcaatct catgcggatt gaaacgagtc
      181 acgagacgca tataggttcc catcgccgaa tcagggacgg acattaattt gccgaacata
      241 tcgtctgcac cggtattgat aggcacaata ttgcccgtag acttagacat tctttgtgcg
      301 ccatcggtgc ccggcaaaat gccggtgatg atgccaacca gaggtttttg ccccaaggct
      361 tcttgaagtt tgcgcccagc cacgatgata ttgaacaact gatcggaacc accaacttga
      421 atatccgtct ccatggcggc ggcatcgtat ccttgcatta gggcgtagaa agtctcgtgc
      481 aggtaaatgg gttcgccctt atcgagacgc ttggcaaagt tctcacgagc caagaactgc
      541 tgaaccgtaa agttttgacc gagacggatc aaatcgacca agctgagttt ggagagccat
      601 tcactgttat agcggatttt tgttttagat ttatccaata cgcgaaatgc ctgttcggcg
      661 taggtcttgg cattctcggc gacttgttcc tgcgtcagaa tcggacgcgc tttatttttg
      721 tccgaagggt cgcccaccaa agctgtgtag ttacctatca ggaaggtcac ttcatgacct
      781 aaatcctgaa actgacgcaa tttgcgcata gaaattgtat gacccaaatg caaatctgta
      841 gacgttggat cataaccaca atacacccgc aaaggacgat tttctttttc agcaatcttt
      901 agacgctcac gcagttccga tgccatggct ttttgcaaat cttcgtcgcc atagtcggtt
      961 ccctgcatca ataactcgac ttgttcctcg atgttcattc ttttctccta atgcaaaccc
     1021 tgtttgggtc gtaatttttt atatcatttg agcgctatac aaacaaaaaa cgcgcccgcg
     1081 atgatggggc gcgttcaaat cacaccatcg caaccagtta cgggtggtaa taatagtaat
     1141 agcgactagt gaataagttg ccagacatgc tcggtattat acatcaggaa ttttattgcg
     1201 acaaattgaa agggaagctc cacggtccgc gtacggtgta gaactcatcg ctatacacaa
     1261 gttgttctgc ttgttcctgt gacatctcag cgggcttgtt taggatttgc attgtcaaag
     1321 caccgttaac gtccacacag tctacagaga cgcccgttcc gcgctccagc atggcttgtt
     1381 gaatctttgg cagatacaaa tcacaatatt cgctgggttg tggaggggct gcaattgcat
     1441 cgatattgat ggcggcattg gtcaggtctg catcgggagc aacaacaaag gtcaaaatat
     1501 cacaacgcac accaggctga ccatttgctg attcctgaat gctctgcaat gtggttccgt
     1561 attcttgcag cacaatacca ctataggcta aattcgccgc ccaaatcgac cagtctgacg
     1621 catcgggcaa ggtaaagcaa acttctgcat tgacattctt tccttccatc catgcacggt
     1681 tcatgcgcac atcgatccct gctgcggttt gttgagcagc gcttacattt gcttctgctg
     1741 ttgggtatga cgatgcatca atataggctg tagcggttgg caataactgt ggcaacacca
     1801 cctgcgaatc ttgtgcaggg gcacaagcgg atatcagaat cattaacagc aaagcaatag
     1861 acatcttctt catggacttt gttatctcct ttgacttaac caaataaatc ggtcaacgcc
     1921 tctcgcggac ccggaaactg gaacgtgtac ccagactcaa gcaaccgttt gggctgggaa
     1981 taccttcctt caaggatcaa gacgctcatt tcacccaaaa cgttccgcat taaaaaggct
     2041 gggacgggaa accaatatgg gcgatgcaat acattggcta aaatcttggt gaaatcagcc
     2101 aatgaggtct gcgaaggcgc aatcatatta tagacgccac gtgcgctttc atccgccatc
     2161 aaatagcgga cagcacttac ccaatctttg atatgaatcc aagggaaggg ttgtttgcca
     2221 gaaccaatgg gtccgccaac gaataattgc actggcaaag caatcaattt cattattaca
     2281 ttatctcggg aaagtacagg tggtgtacgc aaaaccaacc tgcgaacgcc taatccttca
     2341 acagattgag tnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2401 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn ncgcgaaagg
     2461 atgaaaactt tatgaccatc cgcaagaagg gcttttgtca gggcggtgcc aagcaagcct
     2521 gtgccgccag agatcatgat attcattttt gctcctgcaa tgatatgtca atgggtgttt
     2581 aaactgattt aacattctag atgtataatc acgcgagtta ccggaacctt atgaacgaac
     2641 ccgtactggt gctcaacgca aattttgagc cgatccacgt ctgttcgaca cgacgggcag
     2701 ttactttgat ccttgcggga aaagccggta tgatcgcaaa cggacgcgga cacatccgca
     2761 ccatatcaca gctcatccct cgtccttcgg ttattcggct ggaatcgcaa atccatcgac
     2821 cacgcccacg cgtcaaatta acgcgccgcg agatctttag gcgcgataat tatacctgcc
     2881 agtattgcgg caaacgcgac acgaacttga cgatcgatca tgtgattccg cgccatatgg
     2941 gtggtaaaca agtttggacg aatttggtaa cggcttgttc gccttgtaat catcgcaaag
     3001 gcgggcgcaa agtggatgaa gcacacatgc atttgctcca tatccccaaa gaaccacctg
     3061 ccgacgcttc ttatattttt gggaaacact tggcagaaaa ccaagaatgg acaccttata
     3121 ttaccggttg gtaaatgaaa atcccctcat cacgaaggga ttttttatta gaagtctagc
     3181 accataaagt cttttgctat ctggatctcg ccctgatagg tctgagatgc ttcggcggca
     3241 atatcgcctt ttttgaatct gccggtggga tagtgaataa gatacagttt atttacttct
     3301 gcccgcgaag cgatttctcc ggcttgagtg gcagaggagt gaccgggcga ttcgcccgtc
     3361 gcttcgtgga tgagcacgtc tgcaccttcg cttaagcgaa caacttcgtc acaaggttca
     3421 gtgtcacatg aatatgccat cactttttgg ctttgttgga aattgacgcg taaaccaatg
     3481 ttgggaatca tatggtggac tggagaggca tgaataataa agtcgggaca atccaacacg
     3541 ggggtcatct cgcggacggg taaacgatag aaaacgaccg ggaaaaagtt tggccactca
     3601 gaccagttat aaaaacccat caagccttca actcgatcaa gagtgtagtg cagaccatag
     3661 atattgagcg gtttttgcct gcccatcagc cacatatcca taagcaaaag cggaatacca
     3721 gaggcatggt cgggatgaaa atgggtgatg ataatgtcgg taaggttatt aaagtcgagc
     3781 ccggcttgct caagccgtag aatgggattg ctcactgaat cgatcagaac ggtacggtca
     3841 tttcccacaa tgaccatgtg tgtgttttcg ctttcgcttg tgggaattgc gtttgaagcc
     3901 ccaaggatga tgacttttgc cataatcaaa tcttaactca aatcccctta attgttaatt
     3961 gtattttcat atgagccata agaaaagcga gctgccttgt ataaaacagc tcgcttttct
     4021 tgaattaatt tccgacaact cttaatagaa tactaggggt gatatatgcc tcaattaagg
     4081 ctgcgattgc aagcaatggc accaccacac caacatatat ctttgcccaa tctgccatga
     4141 gatcgatcat aacctccccc atcgacttgc cattttgcgg tgtgaccaag gcagcaccaa
     4201 tatacaacac tacggcacta ccaattagca aagccgggag ttcaaaaata ccatgcggca
     4261 aaattccagc cagaaagatc ggagcaggtg caatgcctaa aagttccaaa acggcatata
     4321 cgccgccaat caaagcaatg ttgaccatat acagcagaac gcccaagaca ctaaacgaaa
     4381 atatcccagc gagaaatata acagtcatgg aacgggtgtt gttgaaaaat aagaagggcg
     4441 cgcttaattg tccacccagg ctggaaagat ctggtaggtt ttcaataccc ttttcaagct
     4501 ttgccaacgt atcgggcgag gcgctggtaa tcgcttgaga tacattggtt gtaacccagt
     4561 tgtagctcat ccatacactg acaaccgcca ccaaaagcat ccaacccatg gcgggcaatg
     4621 ctcgccgaaa agaagaagca acaacgcggc gataccaatc tccgacagat gctgcgccac
     4681 cggtaaagtt tttcgagaaa gttcgtaaca tccacttgaa gttgagatta tcaatctctc
     4741 gtccaagtaa atattcacgc tcaaagtgtg caatccctac ccgaatcaat agcaaagaga
     4801 taattaacac accggcaacg cccagccata gtacagtctc attgccccaa aacaacatca
     4861 cagcttcgcc ttgcatcaga atcgccactg gaatcacaat gaacgatgct aacaaatttg
     4921 ccgctttcac tgacgtggat tgcaccgaaa taacaatcgc ggcactaacc atcaagaccg
     4981 catgtgcccc agtaagcaaa agcaattgca ccatcacaga ggcgctaggc atggttaagt
     5041 cttgacgaat gatcaacaac atatacaagc caatggaaac atagctggcg atcaacggcg
     5101 tagcagcccc aaccagcaac ttaccaaaat atagttgcca atcatcaaat ggcgcggtca
     5161 acaaaggctc gatcgtgcca cgttctttct cgcccacaaa agactctaat gccaccacga
     5221 gtgaaacagt aataggaaag aatcctatga tcatgataga gaacggaacc agacgatcta
     5281 atatcaggtt ggcgttgtat tggttcaaga aatccacggt ctgttttgca aattcattca
     5341 tcaagaatgg aaaacagaaa gtcagaatcg ccatgggaat caaaacgcgc caatcgcgga
     5401 actggtctcg cagctcacgt tgggctagaa gccaaacagg tcttaattta ttgaacatat
     5461 tcttttccct ttgcagttgc catcaccttc aagtagactt gttccagtgt gcgcgtttct
     5521 tcctgaaagg caactacagg cgcggacaag gaagtgagcc gctgaatcaa ttctggattt
     5581 gactctgctg gcgcttccac ccggactttc aaactggtcg ctgtcttctc gagtaattcc
     5641 atccctttgg gcatttccaa cctactcgca tcccaagcag atgcaaattt gatgacatat
     5701 tcaggagccc ccaaaacgcc tgacttcaac tcatctaatg agccttgaat caaaatcttt
     5761 ccacggtaaa taatcgcgat cttatctgca agaagttcca cttcggtgag gttgtgagag
     5821 cacaacatga tggtcctttg cgtagacttg agcctcgcaa tttcatctct caccaaccgc
     5881 gcagactcgg ggtccatggc agaggtgggc tcatccaaaa gtaagacgct gggttcgtgc
     5941 atcattgcgc gagcaagcgc taatttttgg cgcatcccct tggaatattc cccgatacgg
     6001 cgtttggagg cttctgccaa gccaaagtat tcaagcaggt tttcggtacg tgatttacgg
     6061 agggaagggt cgaggttgta gacttgccca aaaaaatcca tgtactctga accggtcata
     6121 cgcatgtaca aaccgtgatg ttcagtgagc acgcccaccg aagcacggac gtcatgcccg
     6181 ttttttgtca catcgtagcc agctacacgt gcagagccgc ggcttggctg caataaggca
     6241 gtcaacatac gcacggtcgt tgttttgcct gcgccatttt gccccaacaa ggcaaggatc
     6301 tgcccggcac caatggagag atttactcca tcgacagcca taaaatcatg gaattgttta
     6361 ctaagatcgt tggtctcaat catgaggtat ccttttctac taagaaagag gctacgctca
     6421 ttttattacc catcccccac ggctttcctt ctcaaatccc ttacaaaact ggtgcaagcc
     6481 ttctatgccc caggatgctt atagagacaa tgaaaagaat agcaaaaaat agttgatttg
     6541 cattgacgcc attgaccaat gccacgtcga ggcgaaggtt ctcaagcaga aaacgaacta
     6601 aggaatagaa tgctaaagan nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6661 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnc
     6721 ccggaatgcc aaagccgcct gtccatacag agagaatgtc taagggatga gagagatagt
     6781 gactggtggt aagaccaagc tgaacggagg aaagaggcgg agtgaacaca tgccaaagcc
     6841 tggcaccaat ccatgaccac accgtcacag ggacgaagag gtagtagata agctccgcgt
     6901 cttgaaagcg gcgtttggct tcaatggctg cgaccaaggc accaaccgca atgcccaaag
     6961 cgatcaataa cccgttccag cggatcagta aaaagccgag gtttaagccg tctgtcactt
     7021 ggcttccttg gattcttttg atttcttaat gcgttcccgc ccttgtgcgc ggacattcaa
     7081 gatgcgttgg aaagcagtaa tatttgccaa tactgcaatg ataatcaccg cggcgaacgg
     7141 ctggttgaaa accagtaaag gaatcaacac aatatatcgt tcgaggcgcg tgagaattcc
     7201 aactttagca gtaaagccca agccttcagc gcgggctttg acgtaggaga ccaagaccga
     7261 acctgccgcg gcaaagaagg agatcatcac acccgggtag tcagcttttg tcagaaagta
     7321 atagaccaaa ccgccaaaag tgaccaactc tgcatagcga tcactgaccg aatcaacaaa
     7381 accgccaaaa tcactgggtt caccacgcaa acgtgccatc gttccatcaa aggcatcgac
     7441 tagtacagac gccagcaaaa tgaaagcgcc ggttgtcata tgcccttgcg aaatgacata
     7501 ggcgccaacc gttgtccctg ccaaacctaa taatgtgatg gtgttcgggg taagcccaag
     7561 cccgttgagg aaattcccaa tcgggtcgag aataccttta aatatgatac gggcacggtc
     7621 tgagaaagtt ggttttgtat tcatgtctac gtatttttat cgttcttctt ggttacctat
     7681 ttcatcttcg tcactgataa gcacttcacg ttctttgccg ccgccttgag acgggccaac
     7741 gacacccatt gtttctaatt ggtctaacaa acgagccgcg cgtgggtagc caatgcgcaa
     7801 gcgacgttga agcatagaag cagaagcacg ttgttgttgg cggacaatgg aaatggcttt
     7861 ttccaccaaa ccatcgtcct catcttcttc cggttctttc aacaatgttt cccaaggtgg
     7921 aacttcaacc gcatcagtat cccaattctt ttgccaatac gcaatgatac gttcgatctc
     7981 agtatctgtg atcaatacgc cctgtgcg
//
LOCUS       DAOQJE010000029         7967 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_4922_length_7967_cov_6.824444, whole genome shotgun sequence.
ACCESSION   DAOQJE010000029 DAOQJE010000000
VERSION     DAOQJE010000029.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 7967)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7967)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7967
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4922_length_7967_cov_6.824444"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..963
                     /locus_tag="PLT08_01445"
     CDS             <1..963
                     /locus_tag="PLT08_01445"
                     /inference="COORDINATES: protein motif:HMM:NF013125.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lamin tail domain-containing protein"
                     /protein_id="HNE03153.1"
                     /translation="VSDILADQVYTGELSNSGETLTLSDAANKVIDTANGNGGGWPAG
                     SSSTYGTMERTSTATDSDSIWQTNTGVVKNGKNANGGDILGTPKKANSPLPTPTPTPD
                     KTATPTPLPPTATIPPRPIINEILARPGFDWNQDGSTDVFDEFIEIKNLTSVEISLNG
                     WKLSTVDGDSFNLPDVNLKPGDRVVFYGKQTNILLSDGGETVRLSNSSGKIYDAFTYT
                     VARTEDRSFCRLPDGNPGNSWFEDCIPTPNLTNTREGKSPTSPDGTDSSACNLPDTVP
                     LDFLIPECRGYGNNIWDPYYWDQISGVFKKWISSETSKWDSYIE"
     gene            1018..1836
                     /gene="pyrF"
                     /locus_tag="PLT08_01450"
     CDS             1018..1836
                     /gene="pyrF"
                     /locus_tag="PLT08_01450"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: protein motif:HMM:TIGR02127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HNE03154.1"
                     /translation="METFISFLEKRVDDCSSLLCVGIDPHVTDLESPTAASALTFSLN
                     LVKQTARYAAAFKPNAAFFEVFGAEGWTALKQVIEAINEESDRMGSRIPIILDAKRGD
                     ISSTAEAYAKSAFESLGADCITLNPYLGKDSIDPFIGYKQKGVFLLCKTSNKGSGDLQ
                     DVTLSNGETMYIHVAKLAEAWNEKNNIGLVVGSTHIDAMKSIREAAPNLWFLAPGVGT
                     QGGELDAALNAGLRADGKGLLLPMSRSISRAENPGKAAAEVRDEILYWKNTNQK"
     gene            1890..2498
                     /gene="pyrE"
                     /locus_tag="PLT08_01455"
     CDS             1890..2498
                     /gene="pyrE"
                     /locus_tag="PLT08_01455"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: protein motif:HMM:TIGR00336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004588 - orotate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HNE03155.1"
                     /translation="MSSLPSHLTALADGLLDAGCIKFGEFTLKSGLKSPIYIDLRYII
                     SFPKLLETIGAAYLPLLKELNFKRIAGLPYAAIPIATAVSLQGNYPMIYPRKEVKTYG
                     TKAEIEGEYHAGETVVVIDDLATTGGSKFEAIEKLTGVGLVVKDVVVLVDRQSGAKES
                     LEQAGYNLHAVLTITQLLDYWEENGKVSKEKIAETRTFLKGS"
     gene            2502..2705
                     /locus_tag="PLT08_01460"
     CDS             2502..2705
                     /locus_tag="PLT08_01460"
                     /inference="COORDINATES: protein motif:HMM:NF024769.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdeI/OmpD-associated family protein"
                     /protein_id="HNE03156.1"
                     /translation="MTQDSMTIPLDLSSALSADAEAKALFDLLSPSHQREYIKWVEEA
                     KKTETRAKRIQKMMDMLKQGKEN"
     gene            2718..3263
                     /locus_tag="PLT08_01465"
     CDS             2718..3263
                     /locus_tag="PLT08_01465"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HNE03157.1"
                     /translation="MAGIAVNVAVIQNGKILLTQRDDFETWILPSGGVEDGESLAQAA
                     LRETKEETGLDVALTGLVGTYTRISNMPPIHAVLFTATPIGGTITCQPGETIAVDWFS
                     FDQLPEPLSVGHKRRINDAVSGKKGFVVSQEIIFSSMPDKMTRRDLIELRDKSGLSRQ
                     DFYIKMMEDAILKETVEVMGF"
     gene            complement(3287..>3474)
                     /locus_tag="PLT08_01470"
     CDS             complement(3287..>3474)
                     /locus_tag="PLT08_01470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE03158.1"
                     /translation="MCAFWVEAKEKWTKKVIHSYRMLGVCSFLTGSVFTVSASCGIDK
                     RLQAKDNNVIKAKNIFE"
     assembly_gap    3475..3574
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <3575..4416
                     /locus_tag="PLT08_01475"
     CDS             <3575..4416
                     /locus_tag="PLT08_01475"
                     /EC_number="1.3.5.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006222 - UMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="quinone-dependent dihydroorotate dehydrogenase"
                     /protein_id="HNE03159.1"
                     /translation="IGLAAGYDKDGIAIRGLAALGFGHVEVGTVTPKPQPGNPSPRVF
                     RLVEDDAVINRMGFPSRGMEYLEQQLSGGANWNTVLGVNLGKNKETPNEEAVSDYLAL
                     LKKFAAYANYLTINVSSPNTVGLRSLQGREALEGLLTQLHQQRLAEQEKCKKRLPILV
                     KLAPDLSDAELSDALEAILTTNMDGVIVTNTTLSREGLKSSYREETGGLSGSPLTVKS
                     EAVLRQTLKFVNGKIPVVSAGGVMNSEDAKRRLDMGAALVQVYTGLVYRGPGLVKEIV
                     RAL"
     gene            complement(4413..>6542)
                     /locus_tag="PLT08_01480"
     CDS             complement(4413..>6542)
                     /locus_tag="PLT08_01480"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WD40 repeat domain-containing serine/threonine
                     protein kinase"
                     /protein_id="HNE03160.1"
                     /translation="IGWNPRAGCTWQNKDFIGILARMAIPEKIGRYEIKEELGRGGFA
                     TVYRAYDPRFEREVAIKFLPSELMHSDPQFRMRFQREAKIIAQLEHPSIVPVYDVGAE
                     NEQPYFVMRYMSGGSLSERIKARTYTIEEAVKIIENLAPGLDEAHAKNIVHRDLKPAN
                     ILFTEKNVPLIADFGIAKFTQGDSTVDNMTGSAIIGTPAYMAPEQASGEAVDGRVDIY
                     ALGVILYEMVTGKQPYTADTPLGLAIKHVTEPVPRILEANPNLPVWMEKVISTAMAKN
                     PDDRFYSAVELVETIKAFLRGENPQINANVTKKMSPYNSTATFKRTKTAEPKRRNWVV
                     PALVSVGFLAVAGVCAFALVSSGMLQSLLEPVVTPTIAATSTISPATEVTETSEAITA
                     TVEVPPTENVPSLPLMGGADKIAFIKNNDIWVMNVDGSELKQHTNDGLPKFNLQWMPD
                     GKNLLYMTGKTVKTVNIDTEVEEVLMNFVSAEYFEGFSVSPNGKQAAISINRELFIVP
                     FEIETLRAATRKSALLEMKGCFYNDLGIKELQWSDDSTKLAVEFIANSNGSFADAIRV
                     YDVQDCEKISNSKIDEFPAGRFQFSGDLSSFDWDGDLLFFLTSNTRNGGFGDLALYNI
                     FTHKAEKAAPYESNCCYRDAAFSPDGTQVIFAFQDIRLAGNSPINLYIIPADSISTPR
                     KLEPILPMDFFTKRDEAPQPVMRAAQP"
     assembly_gap    6543..6642
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<6643..6968)
                     /locus_tag="PLT08_01485"
     CDS             complement(<6643..6968)
                     /locus_tag="PLT08_01485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE03161.1"
                     /translation="MSKLTPKIILEGTRLTFKTDIAFALNEHPRIVGPRKYKYHSPLV
                     SGEWCAFTNFPWGRGLINFEPQEEALAMETYNTWVKLFELQKYYSWIVDRFHLSTRMY
                     QLMTYQ"
     gene            complement(6965..7840)
                     /locus_tag="PLT08_01490"
     CDS             complement(6965..7840)
                     /locus_tag="PLT08_01490"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNE03162.1"
                     /translation="MMKKLAFLSLLLVSSLACATIEKALTSGATPTPIVIPTIAPSQT
                     AVPIATDIIRKEESDIFCPSNVTEAVDAYNKGLTYENAGDTASAEKAYRDAIALDPNY
                     CDAMDNLALLVRKNGNSDEAIALYENSIKVAPDNQVAHLGLANTYMELEEYDKALAEY
                     NAILAINPENPEGYYGAGRVYFSKEDFKSAIPQFKQAEELYKAEGSSYLIDAQVYLGF
                     SYALIDEFETGRDYLEMVYPQMQDNGYVNYFLGYCYYYGTSIRDEKLAKQYLLRARDL
                     GIDLGSDLETFVNTP"
BASE COUNT         2035 a         1759 c         1917 g         2056 t
ORIGIN      
        1 gtctctgata tcttggcaga tcaagtctac acaggggaac tctctaatag tggtgaaacg
       61 cttacattga gtgatgcggc gaataaggtg attgatactg ccaatggcaa tggcggcggt
      121 tggcccgcag ggagttcctc tacctatggc acaatggaac gtaccagcac ggcaaccgat
      181 agtgatagca tttggcaaac aaatacgggc gttgtaaaga atggcaaaaa tgccaacggt
      241 ggtgatattt taggaacgcc aaagaaagct aattcgccac tcccaactcc cacgcctacg
      301 ccagataaaa cagccacacc taccccgttg ccgccaactg caacgatccc tccgcgccca
      361 atcatcaacg agattcttgc ccgccccggc tttgactgga atcaagacgg ttccaccgat
      421 gtttttgatg aatttatcga gatcaagaat ctcacctcgg ttgagatttc tcttaatggt
      481 tggaagctca gcactgtaga tggagactca tttaacctgc cagatgtaaa cctcaaacct
      541 ggcgaccggg ttgtgttcta cggaaaacaa accaatattc tgctcagtga tggcggtgag
      601 acggtccgcc ttagcaattc aagtggcaag atttatgatg ccttcaccta caccgttgcc
      661 cgtaccgaag atcgatcctt ctgccgtctg cctgatggga accccggcaa ctcctggttt
      721 gaagattgca tcccaacgcc aaacctcacc aacacccgcg aaggaaaatc gcccacctca
      781 ccagatggca cagactcatc agcttgtaat ttgcccgata ccgttccgct ggatttctta
      841 atccctgaat gccgcggcta tggcaataat atttgggacc cctattattg ggatcaaatc
      901 agcggcgtct tcaaaaagtg gatatcctct gaaaccagca aatgggattc atacattgag
      961 taaaatagac cctaaaggct caacctttag ggtctatgat tttttataag gaacaacatg
     1021 gaaaccttca tctctttttt ggaaaaacgc gttgacgact gctcctctct tttgtgtgtt
     1081 ggaatcgacc cgcatgtgac agaccttgaa agtcccaccg ccgcttcagc cctaacgttt
     1141 agcctgaatc ttgttaaaca aacagctcgc tacgctgcgg ctttcaaacc caatgccgcc
     1201 ttctttgaag tattcggcgc cgaagggtgg acggcgttga agcaggtcat cgaagctatc
     1261 aacgaagaat cagaccgaat ggggtcacgc attcccatca tcctggatgc gaagcgtgga
     1321 gatatttcat ccacggcaga agcttacgct aaatcggcat ttgaatctct cggcgcagat
     1381 tgcatcactt tgaatccata tttgggtaaa gattcaattg acccgtttat cggctataag
     1441 caaaaagggg ttttcttatt atgtaaaacc tcgaataaag gctcgggaga tttacaagat
     1501 gtgacattat cgaatggcga gacaatgtac atccacgttg cgaaattagc tgaggcatgg
     1561 aatgaaaaaa ataacattgg tttggtagtg gggtctaccc atatcgatgc catgaagtct
     1621 atccgtgaag cggctccaaa cttgtggttt cttgcacccg gtgtggggac ccaaggcggt
     1681 gagttggatg cagccttgaa cgctggcttg cgagcagatg gaaagggact cttattgccc
     1741 atgtcgcgtt ctatttctcg tgcagagaat ccggggaagg cggcggctga agtacgagat
     1801 gagattctct attggaagaa tacaaatcaa aagtaataaa cgattactgt tgaagattac
     1861 cctcaaaaaa ctcaaataaa ggaataacca tgtcttcttt gccttctcac cttactgctc
     1921 ttgcggacgg acttctcgat gctggctgta tcaaattcgg cgaatttacg ctcaagtctg
     1981 ggcttaagtc gccgatctat attgacctac ggtacattat ttcgtttcca aagcttttag
     2041 agaccatagg cgctgcctat ttgcctttgt taaaagagtt gaactttaaa cgcattgctg
     2101 ggttgcccta tgccgcgatt ccaattgcga cagccgtttc tttacaaggc aactatccca
     2161 tgatctaccc acgcaaagaa gtgaagacct atggcaccaa agctgagatc gaaggtgagt
     2221 atcatgctgg cgagaccgtg gtggttattg acgatcttgc caccaccggt ggaagcaaat
     2281 ttgaagccat cgaaaaattg actggcgttg gcttggtggt aaaagatgtc gttgtactcg
     2341 tggaccgtca gtctggggca aaagaatcac tggagcaggc tggctacaac ctgcatgctg
     2401 tgctgaccat cactcaacta ttggattatt gggaagaaaa cggcaaggtt agtaaagaaa
     2461 agatagcaga gacgcgaacc tttttgaaag ggagttgacg tatgacgcaa gactctatga
     2521 ccattccact cgacctttct tcagcgcttt cagcagatgc cgaagcaaaa gccctgtttg
     2581 atctactttc cccttctcat caacgggaat atatcaaatg ggtggaagaa gcaaagaaaa
     2641 cagaaacccg tgccaagcga attcaaaaga tgatggatat gttgaaacag ggcaaagaaa
     2701 attaagaggt acaaaacatg gccggtattg ccgtaaatgt cgcggtaatt caaaatggaa
     2761 aaattctctt aacacaacgt gatgattttg agacttggat attacccagt ggtggggtcg
     2821 aagatgggga aagccttgcg caagctgcgc tccgtgagac aaaagaagaa actgggctcg
     2881 atgtagctct tacagggttg gttggaacct atacaagaat aagtaatatg ccgcccattc
     2941 atgctgtttt gtttacagct acacctattg gagggacaat tacctgccag cccggtgaga
     3001 cgattgcagt ggattggttt tcttttgacc aactccctga acctctttct gttggacata
     3061 aaagaagaat aaatgatgcc gtttctggaa aaaaagggtt tgttgtttct caagaaatta
     3121 tattttcttc catgccagat aaaatgacga gaagagattt gattgaactt cgtgataaat
     3181 cgggcctttc acgacaagat ttttatataa aaatgatgga agatgcaata ttgaaagaga
     3241 cagttgaagt tatgggattc tgaggaattc agaaaatgat taagctttat tcaaaaatat
     3301 ttttagcctt gattacattg ttgtctttgg cttgtaatct tttatctatt ccgcaagagg
     3361 cggaaactgt aaataccgaa cctgttaaga acgaacagac tcctaacatt ctataactat
     3421 gtataacttt cttcgtccac ttctctttcg cctcaaccca gaacgcgcac atgcnnnnnn
     3481 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3541 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnncgattg ggttggcggc aggatacgat
     3601 aaagatggca tcgccattcg cggacttgct gctctagggt ttggtcacgt agaggtgggc
     3661 acggtcaccc ccaagccgca accgggaaac cccagcccgc gggtgtttcg tttggtggaa
     3721 gatgatgcgg ttatcaaccg catgggcttc ccttcacgtg ggatggaata tttggaacaa
     3781 cagctttctg gtggcgcaaa ttggaatacg gttcttggag ttaaccttgg caagaacaaa
     3841 gagactccca acgaagaagc ggtctctgat tatttggcat tgttgaaaaa gtttgccgca
     3901 tatgccaatt atctgacgat taacgtcagc tcgccaaata cggtgggact tcgcagcttg
     3961 cagggacgtg aagccttgga ggggttgctc acgcaattac atcaacagcg tttggcggaa
     4021 caagaaaagt gtaagaagcg tttgcccatt cttgtgaagt tggcgccaga cctttctgat
     4081 gctgaattga gcgatgcctt ggaagctatc ctcaccacga acatggatgg tgtgatcgtc
     4141 acgaacacca cattgagtcg tgagggatta aagtcaagtt atcgggaaga aacaggcggg
     4201 ttgagtggca gtccgcttac ggttaagagc gaggcggttc ttcgtcagac cttgaagttt
     4261 gtgaatggga aaatcccggt ggtgagtgcg ggcggagtta tgaattctga ggatgcaaaa
     4321 cgccgcctcg atatgggggc ggcgctggtg caggtttata cggggttggt ctatcgcgga
     4381 cccggtttgg tgaaagagat cgttcgcgct ctttagggtt gtgcggcgcg catgacaggt
     4441 tgtggggctt cgtctcgttt ggtgaagaaa tccatgggaa ggatgggctc gagtttgcgc
     4501 ggcgtggaaa ttgagtcagc gggaatgata tagagattga ttggactatt gcccgccaga
     4561 cgaatatctt gaaaggcaaa gatcacttgg gtgccatcgg ggctgaaggc agcatcgcga
     4621 tagcaacaat tgctttcata cggcgcggct ttttcggctt tgtgggtgaa gatgttgtat
     4681 aacgccagat ctccgaaccc gccattgcgt gtgttgctgg tgaggaagaa gagcaggtca
     4741 ccatcccaat cgaaactgga gaggtcgcct gagaattgga aacgcccagc ggggaattca
     4801 tcgattttgg agttgctaat cttttcgcaa tcttgcacat catacacacg aatggcatcg
     4861 gcaaaactac cgttggaatt cgcaatgaac tcaactgcca gcttggtgct gtcatcagac
     4921 cattgaagtt ctttgatacc gaggtcgttg taaaaacaac ctttcatttc aagtaaagcg
     4981 cttttgcgag ttgcggcacg cagtgtttca atctcgaatg gcacaatgaa tagttcacgg
     5041 ttgatgctaa tggctgcttg tttgccattg ggcgaaacac tgaaaccttc gaagtattct
     5101 gctgaaacaa agttcatcaa tacttcttct acttctgtat caatgttcac cgtcttcacc
     5161 gttttaccgg tcatataaag aaggttcttg ccatctggca tccattgcaa attaaacttt
     5221 ggcaagccat cgttggtatg ttgttttaat tcactgccat ctacattcat cacccaaata
     5281 tcattgttct tgatgaatgc aatcttatcg gcgccgccca tcaaaggcag gctcggcaca
     5341 ttttcggttg ggggcacttc aacagttgct gtgatggctt cggatgtttc agtcacttca
     5401 gtagcaggtg agattgtaga tgtggcagca atggttggag taacaaccgg ctctaacaaa
     5461 gactgaagca tgccgctgct aacgagggca aaagcacata cgccggcaac tgcgagaaac
     5521 ccaactgaca ccaaggcagg gacgacccaa ttgcggcgtt tgggttctgc ggttttggtt
     5581 cgtttgaaag tcgccgtact gttataaggc gacattttct tggtcacatt ggcattaatc
     5641 tgcggattct ctccgcgtag aaatgccttg atggtttcga ccaattccac tgcagaatag
     5701 aagcggtcat ctgggttttt cgccatcgcc gtagagatga ccttttccat ccagaccggt
     5761 agattcgggt tggcttccaa aatacgcggc acaggttcag tgacgtgttt gattgccagt
     5821 ccaagtggag tgtcggcagt gtagggctgt ttgccagtga ccatttcata caagattacg
     5881 cccaaggcat aaatatccac acgtccatcc acagcttcgc cagaggcttg ctcgggtgcc
     5941 atgtacgctg gggtgccaat gatggcagag ccggtcatgt tatcgaccgt ggagtcacct
     6001 tgagtgaatt ttgcaatgcc gaaatcggca atcaatggga cgttcttttc ggtaaaaaga
     6061 atattggctg gcttcaggtc gcgatggaca atgttcttgg cgtgcgcttc atccaaccct
     6121 ggcgcgagat tttcaatgat cttgacggct tcttcaatgg tgtaggtccg cgctttgatg
     6181 cgttcggaga gcgagccgcc gctcatgtaa cgcattacaa aatatggctg ttcattttca
     6241 gcccctacat cgtagaccgg cacaatggag ggatgttcca gttgggcgat gatctttgcc
     6301 tcacgctgga aacgcatccg gaattgtggg tccgaatgca tcagctcaga tggcagaaac
     6361 ttgatcgcca cctcgcgttc gaagcgcggg tcgtacgccc gatagaccgt ggcaaaccca
     6421 ccacgcccga gttcctcttt gatttcgtaa cgcccgatct tctctggaat agccatgcga
     6481 gcaagtatac cgatgaagtc tttattctgc catgtacaac ccgcccgtgg attccatcca
     6541 atnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6601 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnttttgata cgtcatgagt
     6661 tgatacatgc gtgtggaaag atgaaaacgg tccacgatcc atgaatagta tttttgcaac
     6721 tcgaagagct tgacccaggt attataggtc tccattgcca acgcctcttc ctgcggctca
     6781 aagttgatca gtccgcgccc ccagggaaaa ttggtaaaag cgcaccattc tccagaaacc
     6841 agcggcgagt gatacttata cttgcgcgga ccgacaatgc gcggatgttc gttgagtgca
     6901 aacgcaatat cggttttgaa ggtcaggcgt gtcccttcga ggatgatctt cggtgtgagt
     6961 ttgctcatgg tgtgttgacg aaagtctcca agtctgatcc aaggtctatg ccgaggtcgc
     7021 gggcacgcag caggtattgt tttgccaact tctcgtcacg aatggatgtg ccataataat
     7081 aacagtagcc aaggaagtag ttaacatagc cgttatcttg catttgcggg taaaccattt
     7141 caagataatc gcgcccggtt tcaaattcat cgatcaatgc gtatgaaaaa ccgagataca
     7201 cctgtgcatc gataagatag ctcgaccctt cggctttata aagctcttct gcttgtttaa
     7261 actgaggaat agcagacttg aaatcttctt tggagaaata caccctgcct gccccgtaat
     7321 agccttctgg gttttctggg ttgatcgcca agatcgcatt gtattcagcc aatgctttat
     7381 cgtattcttc aagttccatg taggtgtttg ccaaccctaa gtgcgctacc tgattatcgg
     7441 gtgctacttt gatggagttt tcatacaaag caatcgcctc atctgaattt ccatttttgc
     7501 gtaccaaaag agccagatta tccatggcat cgcaataatt cgggtccaat gctatggcat
     7561 ctcgataggc tttttctgct gaggcagtat cgcctgcgtt ttcataggtc aaccctttgt
     7621 tgtaggcgtc cacagcttct gtcacattgg atggacaaaa aatatcagat tcttcttttc
     7681 gaatgatatc ggtggcaatg ggcaccgcag tttgggatgg ggcaatggta gggatcacaa
     7741 tgggtgtggg cgttgcgccg gaagtcaacg ctttttcaat cgtggcacat gccagcgaac
     7801 ttaccaaaag aagagataaa aaagccaatt ttttcatcat ttgcctccag tttgtatgtg
     7861 aaaattatct cattcaatgc gcccagaacc tatagtctat ttgtcctgat ttcgattctg
     7921 ataaatttct gaaacgattc gacaacccag ttgtggtata ttcccgc
//
LOCUS       DAOQJE010000030         7874 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_5009_length_7874_cov_4.614657, whole genome shotgun sequence.
ACCESSION   DAOQJE010000030 DAOQJE010000000
VERSION     DAOQJE010000030.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 7874)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7874)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7874
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5009_length_7874_cov_4.614657"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1719)
                     /locus_tag="PLT08_01495"
     CDS             complement(<1..1719)
                     /locus_tag="PLT08_01495"
                     /inference="COORDINATES: protein motif:HMM:NF014910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HNE03163.1"
                     /translation="MSLSQDFISRLNKRFTGDLRLDTASKVLYSTDASAYQIEPLGVA
                     IPKTQEDVQIAVEFAAKYKIPILPRGSGSSLAGQAIGEALILDCSRYLDSILEIDTET
                     HSAIVEPGVILANLNRAASKHGLMFGPDPASAERATMGGVIGNNATGAHSILYGMSAD
                     HLLEAEVILSDGSSATLNSSTFNLQSSNVRLTNIVSTAHEIRTKYSESIKESYPKSWR
                     NSAGYRLNYLLPWSPSAPPQWSGENYPANLAPGAVNLAHLLAGSEGTLAVIRKLKVNL
                     VQKPKHTILGILSYTSIVEACDDVPRLLAMKPSAVELIPQLILRNARSIPAYARQMGW
                     VIGDPAAVLVVEFSGDQLSALKEAVRKLGDVLTIAESPEDQARIWNIRKVGLGILDSR
                     PTSARPIAFIEDCAIPVERLGEFVREVEQILNAHGTFGGIYAHASAGCLHIRPVLDLQ
                     LGEAVRNMRNIIEQVFALTMSLGGAMSSEHGDGIARGEFIERTYGREVTEAMRLLKKA
                     ADPDNLLNPKKLFDAPPMDTNLRYGETYQAQTWKPVLTFEHERGLEGAVESCNGQGVC
                     RKTNGVM"
     gene            complement(1719..3068)
                     /locus_tag="PLT08_01500"
     CDS             complement(1719..3068)
                     /locus_tag="PLT08_01500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE03164.1"
                     /translation="MKKYIQQFSWSILILAIVFPFLIIRVFPKFYHASDISDFYQWSQ
                     AWNLDWHNIYINCERCNYPFIGTMLSGGVMSLLDIENLDKLVNRFRYFLAAFDALNVL
                     LLYWLLNKLNIKYPQFWAGVIGLLPSSWLGSSVWGQIDGVGQSIILLFFILLVWFNHK
                     TDRSVSQFYLFSFLVGFLLSLMTLTKQLIYFSVFSLGVIYLVNILVLRRKKEQTFIAF
                     ILTILTFLLPIVAIDSMLEFRAPFISNIQYILSTGSKHGDIISSIGFNIWVLFSDNLL
                     GSSHTPIAFRFFSLGSFNIIPYQAGIVLFLLLAICFSATFLRKALMLYKTSAFNDSYF
                     VAMALLYLSLINLSFNLTLTGTHERYLYHFYPFIFVALLVLNKYLGSHSYSNLLILFF
                     GSLTYGLFLFAFLNRWIRITSLFIFKEMSIFYLFFLGYLIYILIRLIFPVKLNEVTQ"
     gene            complement(3162..4319)
                     /locus_tag="PLT08_01505"
     CDS             complement(3162..4319)
                     /locus_tag="PLT08_01505"
                     /inference="COORDINATES: protein motif:HMM:NF013381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HNE03165.1"
                     /translation="MPLSSRERVQAAIHHEIPDRVPIIIGASNATGIKMVTYRRLKKL
                     LNFEAPERYLYDFPELGTALIDEPILERLHVDVRGVLDREPQHILERNASREPDSMYV
                     NSWGGGVTKAGPDNWFPSYHPLAETHAIEDLEKYPWPDMNDPTRVAHVRAHAQQLHQE
                     NKYALMGTPWLAFPMERAYEMQRMDKFFLNMGRHPDFVVELLKKTGEMCKTLMGHFLD
                     ECGAVIDIIKIGDDLGMQSSLMLSPKMYRNYVKPIHADYISFIKSKTKAKVFFHTDGD
                     VFPLLPDFVEMGIDILNPIQTSAGKMSNLPELKKQFGKDLIFCGAIDTHRVLPFGTPE
                     EVRAEVKRIIDILGEGGGYMLASVHTILDDVPAENILAMVDAVEEFGWYKK"
     gene            complement(4407..5153)
                     /gene="gpmA"
                     /locus_tag="PLT08_01510"
     CDS             complement(4407..5153)
                     /gene="gpmA"
                     /locus_tag="PLT08_01510"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000301556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004619 - phosphoglycerate mutase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HNE03166.1"
                     /translation="MYKLVLVRHGQSTWNLENRFTGWTDVDLTDLGKAEAAEAGKLLK
                     EAGYDFDIAYTSVLKRAIKTLGVIQDVMNRDWLPVIRAWQLNERHYGSLQGLNKSEMA
                     EKFGEEQVKIWRRSYDVPPPALELTDERHPKFDRRYASLTPEQLPATESLKITLERVL
                     PYWTSTLAPEIKSGKRLLVVAHGNSIRALVKYLDNISESEITELNIPTGLPLVYELDK
                     DLKPIKNYYLGDAEEAAKKAAAVANQGKAK"
     gene            5303..7873
                     /gene="mutS"
                     /locus_tag="PLT08_01515"
     CDS             5303..7873
                     /gene="mutS"
                     /locus_tag="PLT08_01515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HNE03167.1"
                     /translation="MSNEDVTPVRRQYLEAKKQYPDTIVFFRLGDFYETFDEDAEITA
                     RELDIVLTSRPIGGGIRVPLAGIPYHAVENYLSRLIEKGYHVAICEQIGEVPAKGLVE
                     RKVVRVVTPGTVTEPGLLPGDANNYLASVVMEASASSSGTVASVAYTDVTTGEFAVTE
                     LPLEALRAELTRLHPAEILYPDNQKLPDGIIGHTTHVPAWKFEPGKCNEVLLTQFKAS
                     TLDGFGLKANSLSVRAAGAIIQYLKETQPDALHLLNSLRSYSLNEFMTLDASTRRNLE
                     LDETLRGERKGSLLGTLDATITAMGKRMIHQWVSQPLLNVERINKRQNGVEYFVQNGM
                     VRAELREALRPIADLERLINRVIAGHAQPRDLVAMRSTFANLPNILNALKEKGNKKEA
                     QLPTIDLLGEQLSILQNAIDEDPPATLQNTGVIRPGYSTELDGVIDASKHAREWIANL
                     ESVEREKTGIKTLKVGYNKVFGYYIEISRGAADKAPEHYIRKQTLVNAERFITPEMKE
                     YETLVLNAEERIKEIETRLFKEVCGDLSKTANKILATARSIAELDVLSALGEVAALNG
                     YTKPQVHEGSELEITEGRHPVVEQLLKSDRYIPNDIIFEKGEIVRVITGPNMSGKSTY
                     LRQTALIVLMAQMGSFVPAGHAKIGLVDRIFTRIGAQDEIHAGQSTFMVEMVETANIL
                     HHATNRSLLILDEIGRGTSTYDGLSIAWGIIEYVHNHPQLRAKTLFATHYHELTQLAE
                     LLPGVRNYNVAVSEADGKVVFLHKIIAGGADKSYGIHVAQLAGLPAPVIGRANEIMTE
                     LERSAARGVNVNPNAAQQVALFPETNPLLDELKGIDVNSLSPIEALNKLFEWQKRFGK
                     "
BASE COUNT         2366 a         1848 c         1682 g         1978 t
ORIGIN      
        1 catcacaccg ttcgttttac gacatacgcc ttgcccatta catgactcaa ctgccccttc
       61 caaacctcgc tcgtgttcaa aggtcaatac aggcttccaa gtctgtgcct gatacgtctc
      121 accataccgt aagttcgtat ccatgggtgg cgcatcgaac aattttttag ggttcaaaag
      181 attatcgggg tctgcggctt tcttcagcaa gcgcatggct tcggtcactt cacgcccgta
      241 agttcgttca atgaattccc cacgcgcaat tccatcgcca tgttcgctgc tcatggcacc
      301 gcccaaagac atggtcaacg caaaaacttg ctcaataatg tttcgcatgt tccgcacagc
      361 ttcacccagt tgcaaatcta acacagggcg aatatgcaaa cagcctgccg aagcatgtgc
      421 ataaattccg ccaaaagtcc catgagcatt aagaatctgt tcaacttcac gaacaaactc
      481 ccccagcctt tcaacgggaa tagcacaatc ttcaataaat gcaatcggtc tggcagacgt
      541 tggacgtgag tctaaaatcc ccaaccccac tttgcggatg ttccaaatgc gagcctgatc
      601 ttcaggcgac tctgcaatag tcaaaacatc accaagctta cgcaccgctt ctttcaaagc
      661 cgacagttgg tcgccactaa attccactac caacacggct gctgggtctc caatcaccca
      721 acccatctgc cgcgcatacg ccggaatact acgtgcattc cgcagaatca actgcggaat
      781 caactccact gcgctaggct tcattgccag caaacgcgga acatcatcac acgcttctac
      841 gatactggta tacgacaaaa ttccaagaat cgtgtgctta ggcttttgaa caaggttaac
      901 ttttaactta cggataaccg ccaaagtccc ttcgctacct gctaatagat gtgccaagtt
      961 cactgcgcca ggtgctaagt tagctgggta gttttctcca ctccattgcg gcggcgcaga
     1021 tgggctccat ggcaaaagat aattaaggcg ataacccgcc gagttgcgcc aagattttgg
     1081 gtaactttcc ttgatggact cagaatactt tgtacgaatc tcgtgagctg tagaaacaat
     1141 atttgtcaat cgaacatttg aagactggag attgaatgtt gagctattaa gtgtggcgga
     1201 actcccatca ctaagaatca cttctgcttc cagtaaatgg tctgcgctca taccataaag
     1261 tatggaatgt gcgcctgtag cgttgttacc aatgacaccg cccatcgttg cgcgctctgc
     1321 agatgcaggg tcgggtccaa acatgagtcc atgcttggag gcggcgcggt taagatttgc
     1381 aaggatcaca ccaggctcaa caatagcaga atgagtttcg gtgtctattt caagaatcga
     1441 gtctaagtaa cgcgagcaat ccaaaatcaa cgcctcgcca attgcctgcc cagcaagcga
     1501 agagcccgac cctcttggga gaatcggaat cttgtatttc gccgcaaatt caacagcgat
     1561 ttgaacatcc tcttgagtct taggaatagc tacccctaaa ggctcaattt gataagcgga
     1621 ggcatccgtg gagtacaaaa ctttggaagc cgtatccagt cgaaggtcac cagtgaagcg
     1681 tttattaagt ctacttataa agtcttgaga aagagacatc actgcgtcac ttcattcaat
     1741 tttacaggga atataaggcg gattaggata taaattaaat agcccaagaa aaatagataa
     1801 aaaatactca tctctttaaa aataaatagg cttgtgatac gaatccagcg attcaagaaa
     1861 gcaaacaaaa acagtccata tgtcaatgaa ccaaaaaaca atatcaacag attagagtaa
     1921 gaatgcgatc ctaaatattt attcagcaca agtaatgcta caaaaataaa aggataaaag
     1981 tgataaagat acctttcatg tgtacctgta agagttaagt tgaaacttag attaattaaa
     2041 gaaagatata aaagtgccat tgccacaaaa taactatcat taaaagcaga agttttataa
     2101 agcatcagcg ccttcctcaa aaaggtagca gaaaaacata tcgctaaaag cagaaacaat
     2161 acaatcccag cttggtatgg aattatatta aaagaaccca aagaaaaaaa gcggaaggct
     2221 ataggtgtgt gcgaagaacc taaaagatta tcagaaaaca aaacccatat attaaatcca
     2281 atagatgaaa taatatctcc atgtttgctt cctgtagaca ggatgtattg gatattagag
     2341 ataaaaggtg ctctgaattc aagcattgag tcaatggcta ctataggcaa caaaaaagtt
     2401 aagattgtta aaataaaagc gataaaagtt tgctcctttt ttcgtcgcaa aacaagaatg
     2461 ttgaccaagt agattacccc aagcgaaaat acgctgaaat aaattaattg ttttgtcaga
     2521 gtcattaatg acaataaaaa acccacaaga aacgaaaata aataaaactg acttaccgac
     2581 cggtcagttt tatgattgaa ccatactagt aaaataaaaa ataataaaat tatagattgt
     2641 cctactccat caatctgtcc ccaaaccgag gaccccaacc aggaagaagg taataggcct
     2701 ataactcctg cccaaaactg gggatatttt atattcaatt tatttagtaa ccaatacaac
     2761 aatagaacgt tcaaagcatc aaaagcagct agaaagtaac ggaaccgatt aactagttta
     2821 tcgagattct caatatcaag taaactcatc acgccaccag aaagcattgt tccaataaaa
     2881 ggataattac atctttcaca gttaatataa atgttatgcc aatccaaatt ccacgcttgt
     2941 gaccattgat aaaaatcact tatgtcactg gcatgataga actttggaaa aacacgaata
     3001 atcaaaaacg gaaaaacaat agctaatatt aaaatcgacc atgaaaactg ctgaatatat
     3061 tttttcaaga ggcacctatt aaggcgattc taccaaatag aacaagctat tccgttgatt
     3121 ggtcaacgga atagcttgta aacaaataat taaagcgcaa attacttctt ataccagcca
     3181 aattcctcta cagcatcgac catggcaaga atattctcag cagggacatc atccaaaata
     3241 gtgtggacgg atgccagcat atagccaccg ccttcgccaa ggatgtcaat gatgcgtttc
     3301 acttcggcac ggacttcttc tggtgttccg aatggaagga cgcggtgggt gtcaatcgca
     3361 ccgcagaaga taaggtcttt gccaaactgc tttttaagct ctggtaagtt ggacattttt
     3421 ccggctgacg tttgaattgg attcaaaata tcaatgccca tttcgacaaa atctggcaaa
     3481 agcgggaaaa catctccatc agtatgaaag aatactttgg cttttgtttt cgacttgata
     3541 aaagaaatgt aatctgcatg aatgggcttt acataattgc gatacatctt aggcgaaagc
     3601 ataaggctgg attgcatccc caagtcatcg ccaattttga taatatcgat cacagcccca
     3661 cattcatcaa gaaaatgtcc catgagggtt ttacacattt cgccggtttt cttgagaagt
     3721 tctacaacaa aatcagggtg tcgccccata ttgaggaaaa acttatccat gcgctgcatc
     3781 tcataagcac gttccattgg aaatgcaagc caaggggtgc ccattagagc gtatttgttc
     3841 tcttgatgaa gttgctgggc atgagctcgt acatgcgcaa cccgggtagg gtcgttcatg
     3901 tcgggccagg ggtatttttc gaggtcttca attgcatgtg tttcagccag tggatgataa
     3961 gagggaaacc aattatcggg tccggctttg gtaacgcccc caccccacga gttgacatac
     4021 attgagtcgg gctcgcggct ggcgtttcgc tcaaggatat gttgcggctc acggtctaac
     4081 acaccgcgca catcaacgtg taagcgttca agaataggtt cgtcgatcag ggcagtccca
     4141 agttctggaa aatcatacaa ataacgttcg ggggcttcaa aattaagcag tttcttcaaa
     4201 cggcgatatg tcaccatctt aatcccagtc gcattgcttg caccaataat aatgggaaca
     4261 cgatctggaa tttcatgatg aatggctgct tgaacacgtt cacgagatga aagtggcata
     4321 acattctcct tcggcaaatt aaaagaggac aatgctggtt attcaccatc attgtcctct
     4381 gagttgaatt gtttacttac tacgtattac ttcgctttgc cctgattggc gaccgcagca
     4441 gctttcttag cggcttcttc agcatcgcca agataatagt ttttgatggg tttcaaatct
     4501 ttatcaagtt catataccaa gggcaaaccg gttgggatgt ttaattccgt gatttcgctt
     4561 tcggaaatgt tatcaaggta tttcaccaac gcacgaatgg aatttccatg ggcaacgaca
     4621 agcaagcgct tgccagattt aatctcaggc gccaaggtgc ttgtccagta agggagaaca
     4681 cgctccagcg taatcttaag tgactcggtg gctggcaact gttcgggagt cagtgaggcg
     4741 tagcgtcgat caaattttgg atgacgttcg tcggtcaatt ccaaggctgg aggcggaaca
     4801 tcgtatgaac gtcgccaaat tttcacttgt tcttcaccaa atttttcagc catttcagat
     4861 ttgttcaaac cttgtaatga accgtaatga cgttcgttca actgccaagc gcggataacc
     4921 ggcaaccaat cgcgattcat aacatcttga ataacgccca aggttttgat ggcgcgcttg
     4981 agaacagacg tataggcgat atcaaaatca tagcctgctt ctttcaaaag cttgcctgct
     5041 tcggcagctt cagccttgcc aagatcggtc aagtcaacat ccgtccagcc ggtaaaacgg
     5101 ttttcgagat tccaagtgct ttgtccatga cggactaaaa cgagtttata cattgtggct
     5161 cctaaaaaat tgcccgaagg caaagatatt ttatgttcgc gggtagatta tagtccaact
     5221 ccaaggctca aagtgccaaa atgtgctttc tcgtaacaat acatgtaatt tgtcataaca
     5281 atgggtatag taaaatcagg gaatgtccaa cgaagatgtc acgcctgtcc gcaggcaata
     5341 ccttgaagcc aaaaaacaat accccgatac catcgtcttt tttcggctag gcgattttta
     5401 cgaaaccttc gacgaagatg ccgaaatcac tgcccgcgag ttggatatcg tcctcacctc
     5461 acgcccgatc ggcggcggca tccgcgtgcc gttggcgggc atcccatatc acgccgttga
     5521 aaattatctt tcccgcctga tcgagaaagg ctatcacgtt gccatctgcg aacagatcgg
     5581 cgaagtaccg gctaagggat tggtggagcg caaagttgtg cgtgtggtca ccccagggac
     5641 ggtgactgaa cctggactct tgcctggcga cgcgaataat tacttagcct ctgtggtgat
     5701 ggaagcttcc gcctcatcct ccgggacagt tgcctctgtt gcttataccg acgtcaccac
     5761 tggagaattc gccgtcaccg aacttccgct cgaagcctta cgcgcagagt tgacacgcct
     5821 gcaccccgct gaaattcttt atcctgacaa tcaaaaactt cccgatggaa tcatcggtca
     5881 taccacgcac gtccctgctt ggaagttcga accgggtaaa tgcaatgaag tacttctgac
     5941 tcaattcaaa gcctctacct tagatgggtt tggactgaaa gccaatagcc tctccgtccg
     6001 cgctgctgga gcaatcattc agtacctgaa agaaacgcag ccagacgctc tccatctgct
     6061 gaactcgctc cgctcttata gcctaaacga gttcatgacc ttggatgcat ccacacgccg
     6121 aaaccttgaa ctcgatgaaa ccctgagagg cgaacgcaaa ggttccctgc tcggaacact
     6181 cgatgcaacc atcacagcca tgggcaagcg catgatccat cagtgggtca gccagccttt
     6241 actaaatgta gaacgaatta acaaacgcca aaatggcgtg gaatattttg tgcagaatgg
     6301 tatggtccgc gctgaattgc gcgaggcatt acgccccatc gctgatttag agcgtctcat
     6361 caatcgtgtg attgctgggc acgcacagcc gcgcgacctt gtggcaatga ggtcaacgtt
     6421 tgcgaattta ccaaatatcc tcaatgccct aaaagaaaag ggaaacaaga aagaagcgca
     6481 attacccaca attgatttgc tgggcgaaca attatccatt ttacaaaatg ccattgatga
     6541 agacccgcca gccacactac aaaacaccgg tgtgattcgt cccggctact caactgaact
     6601 tgacggtgtt attgacgcct ccaaacacgc ccgtgaatgg attgccaacc tagagtccgt
     6661 cgaacgcgaa aaaacaggta tcaagaccct caaagttggc tacaacaaag tctttggcta
     6721 ttacatcgaa atttcgcgtg gcgccgccga taaagcccca gaacattaca tccgcaagca
     6781 gacattggtc aatgccgagc gtttcatcac acctgagatg aaagaatatg aaacgctggt
     6841 cttgaatgcc gaagaacgca tcaaagaaat tgaaactcgt ttattcaaag aagtttgcgg
     6901 cgatctctca aaaactgcaa ataaaattct tgccaccgcc cgctctatag ccgaactgga
     6961 tgtactgtct gctctaggcg aagtcgcagc cttgaatggg tataccaagc cccaagtcca
     7021 tgaaggaagc gagttggaaa tcacagaggg cagacaccca gttgttgagc aattattaaa
     7081 gtctgaccgt tacatcccta atgacatcat ctttgaaaaa ggcgagattg tgcgggtgat
     7141 cacgggaccg aatatgagtg gtaagtctac atatttacgt cagaccgctc tgattgtgtt
     7201 gatggcgcaa atgggttcgt ttgttccggc agggcatgca aaaatcggtt tagtagaccg
     7261 tatcttcacc cgcattggcg cacaagatga aattcatgct gggcaatcaa cctttatggt
     7321 tgaaatggtt gaaaccgcaa atattttgca tcacgccaca aatcgcagtt tgctgatttt
     7381 agatgaaatc gggcgcggca cttcgactta tgatggtctg tctattgcat ggggaatcat
     7441 cgaatacgtt cataatcatc cgcaactgcg agccaagact ttgtttgcaa cccactatca
     7501 tgagctaaca caactggcag agttattacc gggtgtgcgt aactacaacg ttgcggttag
     7561 tgaagctgat gggaaagttg ttttcctaca caaaatcatt gccggtggcg cagataaatc
     7621 gtatggtatt cacgttgctc aacttgcagg tcttcccgca cctgtgatcg gtcgtgccaa
     7681 tgaaattatg acagaactcg aacggtccgc tgcacgtggc gtgaatgtca accctaatgc
     7741 agctcaacag gttgcactct tcccagagac caatccactg ctggatgagt tgaagggaat
     7801 tgacgtcaac tccctgtcac ctattgaggc gctaaacaag ttattcgagt ggcaaaagag
     7861 atttgggaag tagg
//
LOCUS       DAOQJE010000031         7850 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_5036_length_7850_cov_6.725850, whole genome shotgun sequence.
ACCESSION   DAOQJE010000031 DAOQJE010000000
VERSION     DAOQJE010000031.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 7850)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7850)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7850
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5036_length_7850_cov_6.725850"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1443)
                     /locus_tag="PLT08_01520"
     CDS             complement(<1..1443)
                     /locus_tag="PLT08_01520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HNE03168.1"
                     /translation="MSSNKIRVVGARVHNLKNITVEIPRDKFVVITGLSGSGKSSLAF
                     DTIFAEGQRRYVESLSAYARQFIGQMDKPDVDYIDGLSPAVSIDQKSTSHNPRSTVGT
                     VTEIYDYMRLLFARAGIPHCPVCGREVSKQSAQEIVDKIANLAEGTRILILAPLVRGR
                     KGTYQAVFEEIRKAGFTRARVDGNVYSLDDEIELDRYKQHTIEAVVDRLVISHEGSKE
                     DKKAALTRLTDSIETALKFGEGYITVNLVDKKEDVAYSEHLACPEHGSTVPEIEPRTF
                     SFNTPQGACPDCQGLGSKLEIDPVRIIPDDSVSLNDGAIASMEWGPREEGGYYWSTLV
                     TAAKHYKIDLDAPFRDLTKEQQHVILYGTGDKQLPMKYTNSSGFEVKFSRAFEGVITN
                     LERRYKETNSEYIREKISEYMSDRPCPSCGGKRLNPAALAVTVDNVNIVEANSWPVLQ
                     TLEWVKKISGKGSPLTNKQKTIAERVIREIH"
     gene            1647..4157
                     /locus_tag="PLT08_01525"
     CDS             1647..4157
                     /locus_tag="PLT08_01525"
                     /inference="COORDINATES: protein motif:HMM:NF013124.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HNE03169.1"
                     /translation="MSTKNKVSTEQSSISPELASTIPSNLPVLRTKLIGREAEQKTVL
                     DLLNQTDIRLVTLVGFGGAGKTTLALHTSHALFEKFPGGLFFIDLTSIYEPALILPTI
                     AATLGLQEEPSREIADTLRDFLSNRSILFVLDNFEQLISGANIIASFLDANPHIRLLV
                     TSREALHLRGEHTLSLAPLESADAMQVFTQYAQTLNPHFRLTEDNTPAITELCNKLDG
                     LPLAIELAAMRTRMFTPQALLARLTSDLETGSPLLATLTSGPRDMPERQRTLRNMVAW
                     SYNLLTDKEKHMLQAAALFRSGFGIGSLAQVTAVPENEAEHILASLVDKNLIQPFHGD
                     EVRFHLLESIREFALEQALKQEDVNTLTEKFVGYYLALARDAEQGFRSPEQVQWLDLL
                     DDELANIQTALRFALNDGYAGSFFQSGYEMLIHLQRYWLLRAHYAVGVSWLEKAKAII
                     DRQVAISSGVISNDLLYQKSKIYGFLGYAYWIMGRYAIAKDLHEISLTAARTLNREDL
                     LWESLNNSAVNLEYMGEFEAAMTYYAQALEIIKRTGDRWQEMRLLINLGNSYVGIEEY
                     ETGRTYLEDALLLTMELKDDYYQAACLQGLGYLELRLRRHKEAEENFLKSLSLFERMD
                     VPFIYTWALVSLARVLGETSKHKETAQVVLKCVEMLETQKDRNLYRTMFETLVAFCIS
                     LNKNDMAARFIGFIEQNYAGESEVILPADKAIFDAKTQQVGSMMDQREYVLEKETGAK
                     GSASLLFQLASGICDEILNDTHSKPVLLELLTERELSVLRLLSQGRTNEEISKELVVV
                     LKTVEKHVANILRKLGAKNRTEAAIWYLENTSNLRQND"
     gene            complement(4203..5105)
                     /locus_tag="PLT08_01530"
     CDS             complement(4203..5105)
                     /locus_tag="PLT08_01530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE03170.1"
                     /translation="MKRQTLVVILNLALTFSLFGSVSIAHAQTTCTDATGATSPCPPS
                     GGDSGSNEEDDNGSNQPGGGNPNPPTDITATPSPTPSPEPTSTPLPLQAQPTLDPTKN
                     APVFEVTPTPTAEPVITSSGNDGATEGENDWEDVCTFDLCLGLTAACYWDGGTPEIVE
                     DGSGNTGVHCTIPDDDDGAAAPFPWGPVGIVGIVVLLMVMLLPAVQKFKNKPDTPQGR
                     SWDTFGDIKGDNVAPTSTPQTREHILLAKQQDGNTSGASKPKPKPKPAPQPTPPPAQP
                     KPPSSGTPGDFNSDGTVDGNDFIP"
     gene            5304..5867
                     /locus_tag="PLT08_01535"
     CDS             5304..5867
                     /locus_tag="PLT08_01535"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HNE03171.1"
                     /translation="MEYHEEDVLMRASQGDKDAFGELYERYAERIFNYVYYRTGNQHD
                     AEDLTARVFQRAMNHIRNYTDRGVPFSAWLYRIAHNLVANWHRDRSRKQEIPIDDLPI
                     LPTKGDHPERNLVRSQEQDTLLKMIRKLPPERQNLLILKFVENMSNAEIGKIMGRSEG
                     AVKSLYHRTLLALRDQLEDQNLNLEGE"
     gene            5870..6718
                     /locus_tag="PLT08_01540"
     CDS             5870..6718
                     /locus_tag="PLT08_01540"
                     /inference="COORDINATES: protein motif:HMM:NF014679.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005504 - fatty acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HNE03172.1"
                     /translation="MLRIVTDGAVDLPTEWYKEYDIHRIPINVHFGEEKTFIQDVELD
                     QDGFYKLVDGKTLPHPKTSQPSPHQFAEFYKKIAQPGDTILSIHITSKLSGTYASSVA
                     AAEELKGTYTVVPVDTLCGTMGTAFMCRSARQMERAGKSVDEIKTFIEGVRSKTHVIL
                     TLETLEYAKRSGRVGTLSAALASVLNVKPIAMLKDGIVDMVDRVRTRKGAIDRVLDLA
                     KENVGDSPVHIGVVHARDEASAKSLLEEAQKRFNVKESFLTDLSISLVINFGPGTVGL
                     VLYPAE"
     gene            6728..6997
                     /locus_tag="PLT08_01545"
     CDS             6728..6997
                     /locus_tag="PLT08_01545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE03173.1"
                     /translation="MSENQTFTSQDTDLQFLESHLSRTLRRVPPPNGLVERLRGRVQM
                     PTRSEIRLRFSDWRRLFVVFGGVMSGMLVLVTLARAFYYLVGRKA"
     gene            complement(7053..7382)
                     /locus_tag="PLT08_01550"
     CDS             complement(7053..7382)
                     /locus_tag="PLT08_01550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE03174.1"
                     /translation="MNNIELEIQREFESAQRARANNNEGQARVCARRAAGIAIRDYLT
                     RKGTPLPSRSAHDLLNFIKDEAPLPSNLKLVADHLTLRVTEEFKLPVDADLVAEAKTL
                     CDWLLKN"
     gene            7423..>7850
                     /locus_tag="PLT08_01555"
     CDS             7423..>7850
                     /locus_tag="PLT08_01555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE03175.1"
                     /translation="MRLKKFHVLTWIVLLSIFVSACGGVLPIGGETTQPGTDATEPSI
                     EEIPSGNGPTVVTGSVEYTDLFFTMGVSEPIVILEDQGGFVARDRKFVIPVESQVLGQ
                     ITSDFYTSPFTYSLTLPAEPKGTLNDVDHDGQKDTGVMIFA"
BASE COUNT         1936 a         1763 c         2025 g         2126 t
ORIGIN      
        1 atgaatttca cgaatgactc gttcagcaat ggtcttttgt ttattggtaa gcggtgagcc
       61 tttgccagaa atttttttga cccattcaag cgtttgtagc acaggccatg aattcgcttc
      121 cacaatgttc acattatcta cagtcacggc aagagcagcg gggttgagac gtttgcctcc
      181 gcacgatggg catggacgat cggacatgta ttctgagatt ttttcgcgga tgtattccga
      241 attggtctct ttgtaacggc gttcgaggtt ggtgataacc ccttcaaatg cgcggctgaa
      301 cttgacttca aatccgctcg agttggtgta tttcattgga agttgtttgt cgcctgtgcc
      361 gtagagaatg acatgttgtt gttcttttgt cagatctcta aatggagcat caagatcaat
      421 cttgtaatgc ttagcggcgg tgacaagtgt gctccagtag taaccgcctt cctcacgcgg
      481 accccactcc atactggcaa ttgctccatc gttcagtgac acactatcat cagggatgat
      541 gcgaacggga tcaatttcca acttggaacc aagcccttgg cagtcagggc aggcgccttg
      601 aggcgtgtta aaggaaaagg tacgcggctc aatttcgggc acggttgagc cgtgttctgg
      661 gcaggcgaga tgctccgagt aggcgacatc ttctttttta tcgacaaggt tgacggtgat
      721 atatccttca ccaaatttga gagctgtttc aatcgagtct gttagacggg tgagcgcagc
      781 tttcttatcc tcttttgaac cttcatgcga aatgacgaga cgatctacta cggcttcaat
      841 ggtgtgctgt ttgtaacggt caagttcgat ctcatcatcc agcgagtaga cgtttccatc
      901 gacgcgggca cgggtaaaac ctgctttacg gatttcttca aagacggctt ggtatgtacc
      961 tttacgtccg cgcactagtg gagcaaggat gaggatgcgt gtaccttcag ccagattagc
     1021 gatcttatcc acaatttctt gtgccgactg tttgctcact tcgcgtccgc agacggggca
     1081 gtgcggaatg cccgcgcgcg caaacaagag acgcatgtaa tcgtatatct cggtcactgt
     1141 gccaacggta gaacgcgggt tgtggctggt ggatttctgg tcgatggaaa cggcagggga
     1201 gagcccatcg atataatcga catcgggctt gtccatctga ccgatgaact ggcgtgcata
     1261 tgccgaaagc gactcgacat aacgcctctg accttcggcg aagatggtat caaacgccag
     1321 cgacgatttg cccgaacccg aaagcccagt gatgacgacg aacttatcgc gtggaatttc
     1381 cacggtgata tttttcaaat tatggacgcg tgcgcccacg acgcggattt tattggatga
     1441 catgagactc ctgtttggga gaaggtatta tagcacaggt gttctggtaa tcgtagtccc
     1501 tggcaaaaag aaaaaatgat tataccaatc agggtgaaca cgccttctca tttcatgtaa
     1561 attgtggtgt cttacaaaat catacttttt ggggagtaag ttgtgccata aaaaagagac
     1621 caacccgtta taatttttta ctgaccatgt ctacaaaaaa caaagtatcg acagagcaat
     1681 caagcatctc gcctgagctt gcttctacga tccctagtaa cttgcctgtc ttgcgcacaa
     1741 agttgatcgg acgtgaagca gagcagaaga ctgtcttgga tctcctaaac caaaccgata
     1801 tacgtcttgt gacccttgtt gggtttggcg gtgcgggcaa aaccacgttg gcattgcaca
     1861 cctcacatgc tctgtttgag aaatttcccg gcgggctgtt ctttattgac ctgacttcca
     1921 tttacgaacc cgctttgatt ttgccgacca ttgccgcaac actgggtttg caagaagagc
     1981 ccagccgcga gatcgccgac accctgcgcg atttcctgtc caatcgttct atcctttttg
     2041 tgttggataa ttttgaacag ctcattagcg gcgcaaatat cattgccagt tttctcgatg
     2101 ccaacccgca cattcgtttg ctcgtcacca gccgtgaggc gttgcacttg cgcggtgagc
     2161 acaccctgtc gcttgctccg ctcgaatcgg cagatgccat gcaggtcttc actcaatatg
     2221 cacaaaccct caacccgcat tttcgattaa ccgaagataa cacccctgcc attaccgaac
     2281 tttgtaacaa actggatggg cttccgcttg ccatcgaact tgccgccatg cgcacgcgga
     2341 tgttcacccc gcaagccctg ctcgcccgcc tcacatcgga tctcgaaact ggttcgccac
     2401 ttttagccac tcttacatcc ggtccgcgtg acatgcctga acgtcaacgc accctgcgga
     2461 acatggttgc ctggagttac aacctgctca cagacaaaga gaagcacatg ctgcaagcgg
     2521 cggcgctctt ccgctcgggc tttggcatag gctcactggc gcaggtaaca gccgtccctg
     2581 agaatgaagc ggagcacatc ctcgcctcat tggtggataa aaatcttatc cagcctttcc
     2641 atggagatga ggttcgtttt catctcttgg aaagtatccg cgagtttgcg ctggagcagg
     2701 ctttgaagca agaagacgtg aataccctca cagaaaagtt tgttgggtat taccttgcat
     2761 tggcgcgaga tgccgagcag ggttttcgta gccctgaaca ggtgcaatgg ctggatctgc
     2821 ttgatgacga acttgccaat atccaaaccg cattgaggtt tgcacttaat gatggatatg
     2881 caggttcgtt ctttcagagt gggtatgaaa tgttgataca cttgcagcgg tattggctgt
     2941 tgcgtgctca ttatgcagtc ggtgtatcct ggctggaaaa ggcgaaagcc atcatcgata
     3001 ggcaggttgc catttcatcg ggggttatct caaatgattt gctttatcag aaatcgaaga
     3061 tctatggctt tttggggtat gcatattgga tcatggggcg ctacgcgatt gcaaaagatt
     3121 tacatgaaat atcactgacg gctgcgcgta ccttgaatag agaagatcta ttatgggaaa
     3181 gtctcaataa ctctgctgtc aatctggaat atatgggtga atttgaagcg gcgatgacat
     3241 attatgcaca ggcgcttgag atcattaagc ggacgggtga tcgttggcaa gaaatgcgtt
     3301 tactcattaa tcttggaaat tcatacgttg gtattgaaga atatgaaact gggcgtacct
     3361 atcttgagga tgccctgctt ttgacaatgg agttgaaaga tgactattat caagccgctt
     3421 gtttgcaagg gcttggttat cttgaacttc ggttgcggcg acacaaggag gcggaagaaa
     3481 attttttgaa atcgctatcc ttgttcgaac gcatggatgt gccgtttatt tacacatggg
     3541 cattggtcag tttggcgcgt gttctgggcg aaaccagcaa acacaaggaa accgcgcagg
     3601 tcgttttaaa atgtgtagag atgctggaaa cacaaaaaga ccggaaccta taccgaacca
     3661 tgttcgaaac cctggttgct ttttgtattt cactgaataa aaatgacatg gctgcccgct
     3721 ttattgggtt tattgagcag aattatgcgg gggaaagcga ggttatccta ccggcagaca
     3781 aggcgatctt tgatgcaaag acgcaacagg ttggcagcat gatggatcaa cgtgagtatg
     3841 tgttggagaa agaaaccggc gccaaaggca gcgcttcact tttattccaa ctagcctctg
     3901 gtatctgtga tgaaatctta aacgatacac actcaaaacc tgttttactg gaattactca
     3961 ctgaacgcga gttgagtgta ctgcgtttgc tttcgcaggg caggacaaac gaagagattt
     4021 caaaggaact ggtcgttgta cttaagaccg ttgagaaaca tgtggcaaat attctcagaa
     4081 agttgggcgc caagaaccgc accgaagcgg caatttggta tctggaaaac acaagcaacc
     4141 tacgtcaaaa tgactgacgc gggttgcttg tgtgaaatta ataggtttga gattcgcaat
     4201 tattaaggga tgaaatcatt tccgtctacg gtaccatcag aattaaaatc tccgggagtt
     4261 ccactgctgg gtggcttggg ttgagcaggg ggcggagttg gttgtggcgc gggtttgggc
     4321 ttgggtttgg gtttggaagc gcctgatgtg ttgccatctt gttgctttgc caaaagaata
     4381 tgctcgcgag tttgtggcgt tgaggtgggt gcaacattgt cacccttaat atctccgaaa
     4441 gtatcccaag accttccctg cggagtatct ggtttatttt tgaacttttg aactgcgggc
     4501 aggagcatga ccataagcaa aacaacaatc ccaacaatac caaccggtcc ccaagggaag
     4561 ggagccgcag cgccatcgtc gtcatctgga atggtgcaat gaacgccagt gttgcctgac
     4621 ccgtcttcta cgatctcggg tgtgccgcca tcccaatagc aggctgcggt caagcccagg
     4681 caaaggtcga aggtgcagac atcctcccag tcattttctc cctcggttgc gccatcattg
     4741 ccagaggaag tgattacagg ctcggcggtg ggtgtgggag tcacttcaaa gacaggtgca
     4801 tttttagtgg ggtctagtgt tggttgtgct tgaagtggta ggggcgtact cgtcggctcg
     4861 ggagatggag tggggctggg tgtagccgta atatctgtgg gtgggttggg gtttccacca
     4921 cctggctggt tgctgccatt gtcgtcctct tcattagagc cgctatcacc gccagagggc
     4981 gggcaggggc tggtggctcc ggtggcatcg gtacaggtgg tttgggcatg ggcaatggat
     5041 acagacccga acaaagagaa ggtcagcgcc agattcaaaa ttacaactaa cgtttgtctt
     5101 ttcataaaaa gctcctttgc attggtttta acaataagtg tttgcattat ccccaaagcg
     5161 gttctgcttg tcggtagggt taaccctact cttccccctg ttttttgata ggaaattggt
     5221 catgttatac tgagtgcaac ttctcaaatc cggcggtgtt ttattattca agttccgccg
     5281 gtaaggtttc tcacttgtcg gatatggaat atcacgaaga agatgtgtta atgcgcgcct
     5341 cacaaggcga caaggatgca tttggcgagt tgtatgaacg ctatgcagag cgtatcttca
     5401 actatgtgta ctatcgcacg gggaaccaac acgatgcaga agatttaaca gcacgtgtat
     5461 ttcagcgggc gatgaatcac attcggaatt acaccgatcg gggtgtgcca ttttcggcgt
     5521 ggttatatcg tattgcgcac aatctggttg ccaattggca ccgtgaccgc agccgaaaac
     5581 aggaaatccc catcgatgac ttgcctatcc tgccaacgaa gggtgaccac cctgagagga
     5641 atttagtccg ctcgcaggag caggatacac tactaaaaat gatccgcaaa ttaccgcccg
     5701 aaaggcagaa tttgcttatt ttgaaatttg ttgaaaatat gtccaacgct gagatcggca
     5761 agattatggg tagaagcgaa ggagccgtga agagcctgta ccaccgcact cttctggcgc
     5821 ttcgagacca actggaagat caaaatctta atcttgaagg agagtaagca tgttaagaat
     5881 tgtgacagac ggcgctgtgg accttccaac ggagtggtac aaggaatacg atattcatcg
     5941 cattcccatt aatgtacatt ttggtgaaga gaaaaccttc attcaagatg ttgaattgga
     6001 tcaagatggg ttttataagt tagtagatgg aaaaaccctt cctcatccaa aaacttcgca
     6061 gccttcgcct caccagtttg cggagttcta taagaagatt gctcaacctg gcgatacgat
     6121 cctttccatt catatcacca gcaagctctc agggacatat gcttccagtg tggctgctgc
     6181 tgaggaatta aaaggaacct acacagttgt accagtcgat accttgtgtg gaacgatggg
     6241 cactgccttt atgtgccgtt ctgcccgcca aatggagcgt gctggcaaga gtgtagatga
     6301 aataaagact tttattgaag gtgtgcgtag taagacccac gtcattctca cgcttgagac
     6361 gcttgaatat gccaaacgta gtgggcgtgt aggcaccctt tcagcagcac ttgcttcggt
     6421 attgaatgtc aaaccgattg ctatgttgaa agatggcatt gtggatatgg tcgatcgtgt
     6481 gcgcactcgt aaaggtgcca ttgatcgcgt gctcgacctt gcaaaagaaa atgtgggcga
     6541 tagcccggtt catatcggtg ttgtccatgc gcgtgatgag gcttctgcca agtctctgtt
     6601 agaggaagcg cagaaacgct tcaacgtcaa agaaagcttt ttgaccgacc tttccatctc
     6661 tcttgtaatc aatttcggac ctggaacggt tggattggtg ttgtaccctg ctgagtaatt
     6721 aaattctatg agcgaaaacc agactttcac ttcccaagat acggacctgc aatttcttga
     6781 atcgcatctc tcgcggacct tgagacgggt gccgcctccg aacggcttgg tcgaacgcct
     6841 gcgcggacgc gtgcaaatgc ccacacgttc tgaaattcga ttgcgttttt cagattggcg
     6901 tagattgttc gttgtgtttg gtggcgttat gtctggtatg ttggtcttgg ttacactggc
     6961 gcgtgcgttt tattatctgg ttgggcgcaa agcgtaactt ctgcattttc tacaaaaaaa
     7021 ctcctgaaag atatctttca ggagtttttc ttttaatttt tcaacaacca gtcacataaa
     7081 gttttggctt cagccaccag atcggcatcg acagggagct tgaattcttc agtgacgcgt
     7141 aatgttagat ggtcagcgac caatttcaag ttggagggaa gaggcgcttc atccttgatg
     7201 aaattcagca gatcatgggc agaacggctc ggcaggggag tccctttgcg cgtcagataa
     7261 tcccgaatag caatgccagc tgcacgccgc gcgcagacac gagcttgccc ttcattatta
     7321 ttggctctgg ctcgttgagc agattcaaat tcacgttgga tttccagttc gatgttgttc
     7381 atgctgggat tataattctt gaaatttcca ggaggctggc atatgaggct taaaaagttt
     7441 cacgtattaa cttggatcgt tttgttaagt atattcgtta gcgcttgtgg cggggttttg
     7501 ccgattggag gcgaaacaac acagccaggc acagatgcaa ccgaaccttc cattgaggaa
     7561 attcccagtg gcaatggtcc aaccgtggtg accggtagtg tggaatatac agatttgttt
     7621 ttcacaatgg gcgtttctga gccgattgtt attttggaag atcagggcgg ttttgttgcc
     7681 cgcgatcgca aatttgtgat tcctgttgaa tcacaggtac ttgggcagat cacatctgat
     7741 ttttatactt caccttttac ctatagtctg acccttcctg cggagcctaa agggacattg
     7801 aacgatgtag atcacgatgg gcaaaaagat acaggcgtga tgatctttgc
//
LOCUS       DAOQJE010000032         7783 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_5107_length_7783_cov_5.269539, whole genome shotgun sequence.
ACCESSION   DAOQJE010000032 DAOQJE010000000
VERSION     DAOQJE010000032.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 7783)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7783)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7783
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5107_length_7783_cov_5.269539"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(132..1709)
                     /locus_tag="PLT08_01560"
     CDS             complement(132..1709)
                     /locus_tag="PLT08_01560"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HNE03176.1"
                     /translation="MNIGRYEVIRELGQGGMAIVYLAYDPNIKRQVAIKVLPKQFTFE
                     PQFRARFQHEVEVVAKLENAAIVPVYDSGEHEDQPYIVMRYMPGGTLADRLGNGPIAL
                     TEIEPLFQRIGSALDYAHRSGVVHRDIKPGNILFDSQGEAFLSDFGIAKLAETSVAFT
                     GTGNMVGTPAYMSPEQAMGEKNIDGRSDIYSLGVVLFEALSGKLPFVSDTPMGVAIAH
                     IQEPVPSLLDHNPNLPQSFETIIRKAMEKKPAERYQTASELSQAIRSMGKSSSPGTMI
                     EQPISSTIIESHASPSRVAVPAPNLSSTPPPTPVSKKSIFPKVLGGIGLMGLCLCVVL
                     TGVFASSLLPGLFTTSSTQVSAETPFASATEEPAPTVFVPIVPTTMANAEIAAEGTVD
                     VSSIYDSTFPASLAIDGDPTTSWFSAGPDEDGTTRYVWTGIQEDFIATIDLISNKDNE
                     VAEFRSGYGFESVLIQVYDAQDQLVYEETVSLAGTPDPDIQITPNVVGQWIWIIFSGS
                     EAQDRGGFAELKVGVVR"
     gene            complement(2171..2632)
                     /locus_tag="PLT08_01565"
     CDS             complement(2171..2632)
                     /locus_tag="PLT08_01565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE03177.1"
                     /translation="MPFAYRLDKNIKIFFLVTYGHCTFSDIFSPSLDPETKPENRPRV
                     KFILDNMLGDLEIDMEGMKFFIKAMNELKSTGFQLEPTAFITNKRGLEIFIKSLDLLV
                     DNDVTTHMAFSSLDEALIWLDEVENAAAIKQINDELLESLRLQYGPHPQLV"
     gene            2806..3114
                     /locus_tag="PLT08_01570"
     CDS             2806..3114
                     /locus_tag="PLT08_01570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE03178.1"
                     /translation="MTNQWYSLGLGDGMLAAAPSEEIQVYFAKVFSAMGMPPEMAVFT
                     RHESEGRLHCEVVAYFSPKASEVAQAFDAEPCPKPVRAGLGLLAGSETAWSNLFDEAQ
                     "
     gene            3278..3841
                     /locus_tag="PLT08_01575"
     CDS             3278..3841
                     /locus_tag="PLT08_01575"
                     /inference="COORDINATES: protein motif:HMM:NF013630.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HNE03179.1"
                     /translation="MQYKKLLQIFVLLAVVVSAFGFTGGASAWYCTSYVTVQWGDTLS
                     GIASLCGTTVDAIRAANPGLGWWVYAGQVLYIPTGSTPSYPAPSYGGTYVVQWGDTMA
                     KIAGYYGVTVNDLLAANPQVWSPSLIYAGQVLNIPAAPAYHTVSYGETLRMIAGYYGT
                     TVYSLQLLNPTIYDPNYIYPGQSIRVR"
     gene            complement(3896..4090)
                     /locus_tag="PLT08_01580"
     CDS             complement(3896..4090)
                     /locus_tag="PLT08_01580"
                     /inference="COORDINATES: protein motif:HMM:NF015957.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0080135 - regulation of cellular response
                     to stress [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HNE03180.1"
                     /translation="MNVQRQALRRSRHDRIIAGVCGGLGEFFGISPFWFRLAMFIAFI
                     PGGVPGILIYFIAMLIIPSE"
     gene            4236..4883
                     /locus_tag="PLT08_01585"
     CDS             4236..4883
                     /locus_tag="PLT08_01585"
                     /inference="COORDINATES: protein motif:HMM:NF022152.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgjV family protein"
                     /protein_id="HNE03181.1"
                     /translation="MDSSFIYELIGYAASLLVAFSLTMKSLQKLRIVNMIGAVVFIVY
                     GLLIGAIPVAFLNTLILGVNVYNLWQMWQQKDYFTLMQVRADSAYLKKFLEFYRTEIS
                     EFVPTYQFKPADDQIVVFILRNMVPVGVVIVKPEGEDARIFLDFVIPGYRDFRAGKFL
                     FEESTEFYRQLGIQRLSTAPGSKRHETYLHRMGFKLEEDGRYYYELPIRIIREQI"
     gene            4903..5781
                     /locus_tag="PLT08_01590"
     CDS             4903..5781
                     /locus_tag="PLT08_01590"
                     /inference="COORDINATES: protein motif:HMM:NF012863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HNE03182.1"
                     /translation="MKRSFARFTLFFFFLTTACYQELQTDASLPIVTSVATSGFTPLP
                     PTITVTPTSIFSPAQALAAGPINIIAIGDDFTQGEGDTLGRGYTGLLIELASQIRPGS
                     TIVNFGRTGWDSNDLLNGKDEFSGQLDLAVSEVKSAASQRRAAVVLVWVGGNDLWELY
                     SGTTEVTIEMEEQDALRFAQNMDTIVFELRNAKAEVIIAKLDDQSQRPAKTRGEVYPD
                     ITSDELLRMSMQVQRYNESISIIATKYNALTVDFYNSEIFIQPTTLAADGYHPNPAGY
                     ELISQAWYKALIKILP"
     gene            complement(5816..6481)
                     /locus_tag="PLT08_01595"
     CDS             complement(5816..6481)
                     /locus_tag="PLT08_01595"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF012387.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0016018 - cyclosporin A binding [Evidence
                     IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HNE03183.1"
                     /translation="MFSKKSIFMMIAVATLVFSSFACNAIAGTPQTSDEPAQGNTSTC
                     SLFDSIPAAGQYSSAPDMRINTNGSYTATLTLAKGEEIVIELYASEAPLTVNNFIFLA
                     CKGFYNNTTFHRVLQDFMAQGGDPTGTGMGGPGYEFVNEYSDLTFDKPGVVAMANAGP
                     DTNGSQFFITFGPTEWLNGGYTIFGQVIQGMDAVMSITLRDPDANPSFTGDVLQSVTI
                     SGQ"
     gene            complement(6493..7053)
                     /locus_tag="PLT08_01600"
     CDS             complement(6493..7053)
                     /locus_tag="PLT08_01600"
                     /inference="COORDINATES: protein motif:HMM:NF012797.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HNE03184.1"
                     /translation="MSVRCPICLGGTEVVLNKTIRDEERKIEKSTSKKNGFHAVLLDN
                     IRSAWNVGSIFRSADGFGFDHVYLGGITPTPENEAVVKTSLGAEEYLPWSYHKDAVKL
                     IHGLKKDGWRIVALEEGEGSIQLGKQKSTKAKKSVLILGSEVTGVDPELLALCDQIIS
                     IPMRGQKRSLNVAIAFSVAAYALSNA"
     gene            7149..7709
                     /locus_tag="PLT08_01605"
     CDS             7149..7709
                     /locus_tag="PLT08_01605"
                     /inference="COORDINATES: protein motif:HMM:TIGR00093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HNE03185.1"
                     /translation="MRYILFNKPFDVLSQFTDEGTGHTTLKDFIPVPNVYAAGRLDRD
                     SEGLLLLTDDGALIKRLTDPKHHIEKTYLVMIEGDPTPEKLKQLSSGVQIKDHFTRPA
                     SFRLIPEPALPPRSKPVTPHGPTAWLEVKLREGKKRQIRHMTAAVGLFTLRIFRIAIG
                     EIEIGSLQVGQWRDLTSNEVKLLRRV"
BASE COUNT         1967 a         1734 c         1894 g         2188 t
ORIGIN      
        1 gttcttgcac cggtgtttac ggcgattaag tgggtctttg tgaatggaag ttttgttgtt
       61 cttctcattg gtctgttgtt gttcttgtgg aaacgcttga aaaaatagat ggactggaac
      121 ctgagaagtt tttatcttac aactccgacc ttcagttcgg caaaaccacc gcgatcttgt
      181 gcctcgctgc cagaaaagat aatccaaatc cattgcccga caacattggg agtgatttga
      241 atatctgggt caggcgttcc tgccaagctg acggtctctt cgtagactag ttgatcttga
      301 gcgtcgtaca cttgaattaa cacagattca aaaccgtagc cgctgcgaaa ttcagccact
      361 tcattatctt tgtttgaaat tagatcgatg gtggctataa aatcttcctg aatgcccgtc
      421 catacatatc gggttgtgcc atcttcatcg ggtccggcag agaaccatga agtagtgggg
      481 tctccatcaa tggcaaggga ggcagggaag gtggagtcgt aaatcgagga aacatctaca
      541 gtgccttctg cagcgatctc ggcatttgcc atggtagtcg gcacgatggg aacaaatact
      601 gtgggagcag gttcttctgt cgccgaggcg aacggagtct cggctgagac ctgagtcgat
      661 gaagtggtaa acaagcctgg gaggagactc gaagcgaaaa ctccggtcag aacgacgcaa
      721 agacaaagtc ccattaaccc aattcctcct aatactttgg gaaagatgga tttttttgat
      781 acaggcgttg gaggcggggt tgaggagagg ttcggtgcag ggacggcgac acgactgggc
      841 gatgcatgtg attcaatgat cgtgctgctt attggctgtt caatcatggt tcccggagaa
      901 ctacttttcc ccatagaacg aatggcttgg gacaattcgc tagcggtttg atatctttcg
      961 gctggtttct tttccattgc cttgcggatg atcgtctcaa aggattgtgg caagttgggg
     1021 ttgtggtcta gcaggctggg taccggttcc tggatgtgag caatggcaac tcccataggt
     1081 gtgtctgata cgaaaggcag tttcccgctg agtgcttcga acaacaccac gcctagcgaa
     1141 taaatatcgc tgcgaccgtc tatattcttt tcacccattg cctgttctgg gctcatgtaa
     1201 gctggtgtgc cgaccatgtt gccagtaccg gtaaaggcga cactggtttc tgccaatttt
     1261 gcgatgccaa aatcggaaag gaaggcttcg ccttgcgagt cgaagaggat gttgccgggc
     1321 ttgatatcac gatgcaccac accggagcgg tgggcgtaat cgagcgcgga accaatgcgt
     1381 tgaaagaggg gctcgatctc tgtgagtgct atgggaccat ttcccaaacg gtcggcaagg
     1441 gtgccgccgg gcatgtagcg catgacgatg taaggttggt cttcatgttc ccccgaatca
     1501 tagactggaa cgatggcggc gttctcgagc ttggcaacca cttcaacttc gtgttggaag
     1561 cgggcgcgga attgcggttc gaaggtgaac tgttttggca gaaccttgat cgccacttgg
     1621 cgtttgatgt tcgggtcata cgccagataa acaattgcca tgccgccctg tcccaattcc
     1681 cgtatgacct cataccgacc aatattcatg attgccttcc aataagagcc agtttgttat
     1741 tgcttgacca taattttcga taaactacgt ctgatttatt tcagcgcatt atacattaga
     1801 ttatttcttg gcgattttta tttccaatca ttgatttcaa aacatgattt aataattttc
     1861 gccttgtgta atcaaaattt gaccagatgg gaacgaataa atatccgaag aatgtaccct
     1921 cagggactcg gcatttgagc ctcgcgaggg cgagaggcag gtcgaggggt tgtttgagtc
     1981 catgctggcg gaggtgttca atgggtaggg gcgactcttc aatgccatcg gtcgttatgt
     2041 caagcaagga attcttacca gacaaagcgc gagtcagtcc atggcgagtc gcccctacgg
     2101 ttgcgtttgc gagcgtagtg gcgcaggtcg ctgtgaaggc agatactcga gggtgtgggc
     2161 agtttagctt ttacaccaat tgcggatgag gaccatattg caaacgtaag gattccaata
     2221 gttcgtcgtt aatttgctta atagctgcgg cgttttcaac ttcgtccagc cagatcagcg
     2281 cttcatcgag tgaagagaat gccatatggg ttgttacatc attatctaca agtaaatcaa
     2341 gggatttaat aaatatttcg agccctcttt tattagttat gaaggcggta ggttccagtt
     2401 gaaagcctgt gcttttcaac tcattcatcg ctttgatgaa aaatttcatg ccttccatgt
     2461 ctatttcaag gtcgcctagc atattatcga gaatgaattt gactctgggt cgattttcgg
     2521 gtttggtttc tggatctagt gatgggctga agatgtcgct gaatgtacag tgaccatagg
     2581 tgacaaggaa gaaaattttg atatttttat caaggcgata tgcaaagggc ataagaaatg
     2641 tccacttcgt ggttggggtg ttggttgata acttgtttca agtataacag gcgctgatga
     2701 caatttattt tgtaatattc tagtatagag tgttagactt acatatcaat gcgacatttt
     2761 tagtctgaat tatttgtgcc agtattcaaa atggaaaact ccgatatgac aaatcaatgg
     2821 tattcccttg ggttaggcga cggcatgttg gcagccgctc catcagaaga aattcaagtt
     2881 tattttgcga aagttttttc ggcgatggga atgccgcctg aaatggcggt ttttactcgc
     2941 cacgaatctg aaggacggtt gcattgtgaa gtggtggctt atttttcgcc aaaagcctca
     3001 gaggtggctc aggcattcga tgccgaaccc tgcccaaaac cagtaagagc cgggctggga
     3061 ctcttggcag gcagtgagac ggcttggtcc aatttgtttg acgaagcgca ataaaactcc
     3121 attctaattt cattgcagag ggaatctcta gaggaacaaa agaggactct ttagcgttat
     3181 tagattcata agtacattcg tgtgattgaa ttcgcgcgtg gaatctatat aatttaagaa
     3241 gagtcgtttt tgttttcaac catcaatgga ggatgatatg caatataaga aacttttaca
     3301 aatatttgtt ttgctggcag ttgttgtttc tgctttcggc tttaccggcg gtgcctctgc
     3361 ttggtattgc acttcgtatg tgactgtgca gtggggtgac accctgagcg gaattgcctc
     3421 tctctgcggc accacggtgg atgcaattcg cgctgccaac cctggtttgg gctggtgggt
     3481 gtacgccggg caggtcctct acatcccaac cgggtctaca ccatcgtacc ctgcgccttc
     3541 gtatggtgga acctatgtag tgcaatgggg tgacactatg gcaaagattg ctggttatta
     3601 tggcgtgacc gtcaacgact tgctggctgc caacccgcaa gtttggagcc caagcctcat
     3661 ctatgctggg caagtgctta acatcccggc ggcacctgct taccatactg tctcctatgg
     3721 tgagacattg cgtatgattg ccggatatta cggcacaacg gtttacagcc tgcaattgct
     3781 gaaccccacg atctacgacc cgaattatat ttaccccggt cagtccattc gagtacgata
     3841 aaaacaaaca aaaagaagtc cgctatggtg cggacttctt tttgtttaaa ctacattatt
     3901 cagatgggat gataagcatc gcaataaagt agatcaatat cccgggcacg ccacctggaa
     3961 taaaagcgat aaacatggcg aggcgaaacc agaaggggct aatgccaaag aattcgccaa
     4021 gcccgccaca taccccggcg atgatgcggt catgtcttga gcgtcttaaa gcctgtcttt
     4081 gtacgttcat ttttttctcc tttttatgca gagatatacg cacaaattta cccacggttt
     4141 cagataaaat tacagtagtt gtccagactg gcttgaaatt gaaaaaatta tccagtgtat
     4201 aatgccccgc ttcaaaattc aacgcaggaa gaaaaatgga ctcatctttt atttacgaac
     4261 tcatcgggta tgccgcatct ttgttggtgg cattttcact gacaatgaaa tctctgcaaa
     4321 agctgcgtat tgtgaacatg atcggcgcag ttgttttcat tgtgtatggc ttgttgatcg
     4381 gagccattcc agtggcattt ctcaatacct taatcttggg ggttaatgtc tataacctgt
     4441 ggcagatgtg gcagcaaaaa gattatttca cactcatgca agtccgcgca gatagtgctt
     4501 acctgaaaaa gttccttgag ttctaccgca ctgagatttc tgaatttgtt cctacctatc
     4561 aattcaaacc tgccgatgac caaattgtgg tttttatttt gcgcaatatg gtgcctgtgg
     4621 gtgttgtgat tgtgaagcct gaaggcgaag atgctcgcat ctttttagat tttgtcattc
     4681 ccggctatcg cgattttcgc gcaggcaaat tcctatttga agaaagcacc gagttttatc
     4741 gtcaattggg aattcagcgc ctgtccacgg cgccgggcag caaacggcat gaaacatacc
     4801 tgcaccgcat ggggtttaaa ctggaagaag acggcaggta ttactacgaa cttcccatcc
     4861 gcattattcg agaacaaatt tgatctttca tcgaggcaat caatgaagcg ttcgtttgcg
     4921 cggtttactc tgtttttttt ctttttgaca acagcctgct atcaagagct tcaaacggat
     4981 gcatcactcc ccatagtgac ttccgttgca accagtggat ttacgcctct tcctccgacc
     5041 ataacggtga cgcccacttc gattttttct cccgctcaag ccttggctgc tggtccgatc
     5101 aacatcattg ccatcggcga tgacttcacc caaggcgaag gggatacgct tgggcgcgga
     5161 tacaccggac tattaataga gttagccagc caaatacgcc cgggctctac aattgtcaac
     5221 tttgggcgca ccggttggga ctcgaatgac ctgctcaacg gcaaagatga attttctggg
     5281 cagttagatt tagctgtgag tgaggtcaag tctgcggctt cacagcgtag agccgctgta
     5341 gttctagttt gggtgggcgg taatgacctt tgggaattgt attcaggtac aactgaagtc
     5401 accattgaaa tggaagaaca agatgctctc cgtttcgccc aaaatatgga cacaatcgtc
     5461 tttgagttgc gaaatgcgaa ggcggaggtc atcattgcca agctggatga ccaatcacaa
     5521 cgtccggcaa agacccgcgg tgaagtgtat ccagatatta caagtgatga attacttcgt
     5581 atgtccatgc aagtgcagcg ctataacgaa agcatctcta ttattgccac aaaatataat
     5641 gcccttactg tagattttta caatagcgaa attttcattc aacctacaac gctcgccgca
     5701 gatgggtatc acccgaaccc tgcgggctat gaattgatct ctcaagcttg gtataaggcg
     5761 ctcataaaga ttttgcccta aaagaaaaca gccgccaatt gggcggccgt tttgattatt
     5821 gtcctgagat agttacgctt tgaagaacat ctccagtgaa ggatgggttt gcatctgggt
     5881 cacgcaatgt gatggacatg actgcatcca taccttgaat aacttgccca aagattgtgt
     5941 acccgccatt tagccattct gtaggaccaa acgtaataaa aaactgactg ccatttgtat
     6001 ccggtcctgc attcgccatg gcaaccacac cgggtttatc aaaggtcaaa tcactgtatt
     6061 cattcacaaa ttcatacccg ggtccaccca tgccggtgcc agttgggtca ccaccttgtg
     6121 ccatgaaatc ttgcaagacg cggtggaagg tcgtgttgtt gtaaaaacct ttgcaggcaa
     6181 gaaagataaa attgtttaca gtgagcggcg cttcgctggc ataaagctca atcacaattt
     6241 cttcgccctt tgcgagggtg agagtggctg tatagctccc attggtattg atacgcatgt
     6301 ctggcgctga tgaatactgc cctgctgctg gaatactgtc aaacaagctg cacgtggagg
     6361 tgtttccctg tgcaggttca tctgaagttt gaggtgtgcc tgctatggcg ttgcaagcaa
     6421 aactggaaaa gaccagagtt gcaacggcaa tcatcataaa aattgatttt tttgaaaaca
     6481 tgaaattctc ctttaggcgt tggataatgc gtaggctgct acgctaaaag caatggcaac
     6541 atttagcgag cgtttttgtc cgcgcatggg gatggatatg atttggtcgc agagtgcaag
     6601 taattcgggg tctaccccgg tcacttcact gccaaggatc aacacagact ttttagcttt
     6661 tgtgctcttt tgttttccaa gctgaataga tccttcgcct tcttcaagcg caacaatccg
     6721 ccagccgtct tttttcaacc catggattaa tttgactgca tccttgtgat acgaccaggg
     6781 aagatactct tccgcaccca aagacgtttt tacgaccgct tcattttctg gagtgggtgt
     6841 gattccaccc agatacacat ggtcgaaccc gaacccatct gcgctgcgaa aaatcgagcc
     6901 tacattccat gccgaacgga tgttatctaa caagacggca tgaaagccat tttttttgct
     6961 agtagatttt tcaattttcc gttcttcgtc gcgaatggtt ttgttcaaga ccacttctgt
     7021 cccgccaagg cagatcgggc aacggacact cattgaactg tttttttgta tggggtagcg
     7081 tagcccgcac gaagtgcata cgcggatttc gaagtagatt tcattcatcg ggtatgatta
     7141 tacccagcat gcgatacatt cttttcaaca aaccctttga tgtactttct caattcaccg
     7201 atgaaggtac gggacatact accctaaaag attttatacc agttcccaat gtttatgcag
     7261 cagggcgtct tgaccgtgat agtgaagggt tacttttact tacagatgat ggtgctttga
     7321 tcaaacgcct gacagaccct aaacatcata ttgaaaaaac gtatctagtt atgatagaag
     7381 gggacccaac tcctgagaaa ttgaagcaac tctcaagcgg agttcagatt aaagatcatt
     7441 ttacccgccc tgcttcgttc cgcctcattc ctgaaccagc tttacctcca cgctccaaac
     7501 ccgtcacccc gcatggaccg actgcctggc ttgaagtcaa attgcgtgaa gggaaaaaac
     7561 gccaaatccg ccacatgact gcggcggttg gcttgtttac tttacgaatt tttagaattg
     7621 cgattggaga aatcgaaatt ggaagtttac aagtggggca atggcgagac ttaaccagta
     7681 acgaagttaa gttgttgcgc cgggtttaat tcttcagttt tgactctaag ttcttttgtg
     7741 agcccggctt cagtgacgat tctgggcacg atctcttccc agc
//
LOCUS       DAOQJE010000033         7729 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_5151_length_7729_cov_3.926114, whole genome shotgun sequence.
ACCESSION   DAOQJE010000033 DAOQJE010000000
VERSION     DAOQJE010000033.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 7729)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7729)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7729
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5151_length_7729_cov_3.926114"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(74..1528)
                     /locus_tag="PLT08_01610"
     CDS             complement(74..1528)
                     /locus_tag="PLT08_01610"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNE03186.1"
                     /translation="MIRSLWLKLMGAFLVIVLIGGGIDTYLVSRSTRTQFSQYIDQNG
                     RVFAQQLAPTLAQYYSRQGNWQGVESLLNNPWGGSMMGDGMGMMDEGDWGMWQGWDGM
                     GTNGGMGMGDSNPWNMMGIRLLLADTQGTIIADSAALDTGKTISSADLTTGVPILVGN
                     QQVGTLLPVSAGTNVSGAAGDFVSSVNQSTWLAGGIATLVAFVLGSILFFQIVSPVQK
                     LTSAAQKIAAGDLKQRIPNQSQDEIGMLATAFNQMADSLAKHEELRRSMIADVAHELR
                     TPLTVIQGNLEAMLDGVLPTSPQEIATLRDETALLTRLVADLRLLSLAESGQLKLERV
                     RTNIAELTTHAVEPFHLQAQSSQVELNLEFASNLPQVEVDTDRITQVIRNLLSNALRH
                     TPEGGQVTISCERDTPHQVVITVSDTGEGISPGDLPYVFDRFYRADKSRSRTSGGSGI
                     GLAIVKQLVEAHGGRVWVESQPPQGTTFGFTLPS"
     gene            complement(1518..2216)
                     /locus_tag="PLT08_01615"
     CDS             complement(1518..2216)
                     /locus_tag="PLT08_01615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HNE03187.1"
                     /translation="MADKILVVEDEAHITRTLRLYLEQAGYQVVIISDGALAMPAFRH
                     EKPDLVILDLHLPHVDGWEICRQIRREGDTPIIMLTARSEESDKLIGLELGADDYVTK
                     PFSPREVTARVRVVLRRSRGQVQPPPILRADTLILDLEAHTATHANQPLDLTPTEFDI
                     LTALMRHPGQVFTRLQLVEATQGGAYEGYERVIDQHIKNLRAKLGDDARAPRFIQTVF
                     GVGYRFSAEFRHDS"
     gene            complement(2244..2555)
                     /locus_tag="PLT08_01620"
     CDS             complement(2244..2555)
                     /locus_tag="PLT08_01620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE03188.1"
                     /translation="MKNVTLWIVIGAIALFALGLFGGMFMPFGWNNTAIGYGCDGWNG
                     YGMMGNRGYMPMMGGWGMMPFGFLGMAFMWLVPLGVLALIIGGIFWLVRTLTQKQASP
                     S"
     gene            complement(2587..3300)
                     /locus_tag="PLT08_01625"
     CDS             complement(2587..3300)
                     /locus_tag="PLT08_01625"
                     /inference="COORDINATES: protein motif:HMM:NF019262.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE03189.1"
                     /translation="MKVLKFILGLVAAGVLLVGGFAAGTFITQLRTANMPAQQIAQPT
                     FIPHQQIQPTIVLPQLQVTPAPQTTPPVIPPQGQWGRDWWMMNPNYAPQGQQGWDMHD
                     GDYENWNGMHGGMMNGGMMNGWQTNPNLYLTQTVPNTSPYYPTPSTIVSFRNEVQPIF
                     AARCIACHGGTNGLFLDTYENVLKGSVNGAVVVPTDVYNSRLAYYVYSGYMPFRNTPL
                     NSTEIQTILDWIALGAPNN"
     gene            3452..3799
                     /locus_tag="PLT08_01630"
     CDS             3452..3799
                     /locus_tag="PLT08_01630"
                     /inference="COORDINATES: protein motif:HMM:NF033788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HNE03190.1"
                     /translation="MITVSAPSKTNLQAKLFRGFGDPSRLGILDALRNGPLTVSEIVE
                     ATSLSQPNVSNHLGCLRDCGLVVAEQQGRYVTYHLSDDRVGELLALTESLLADVALGV
                     YECTRYNIPRTEK"
     gene            3813..4298
                     /gene="lspA"
                     /locus_tag="PLT08_01635"
     CDS             3813..4298
                     /gene="lspA"
                     /locus_tag="PLT08_01635"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: protein motif:HMM:TIGR00077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HNE03191.1"
                     /translation="MKPIYRALVSLLIVLSLDWMTKTWVEQVLIPFQPMPLAGQFFRL
                     TLGYNTGVAFGLFANGGVGPLVLTGIIIVGMVIWLVSTLRSGELPPTAAAPIGLILGG
                     AIANFADRLPDGRVTDFLDAGFGTTRFPTFNAADTFIVLGVALLLLVSLTDKQPVGSG
                     G"
     gene            4348..6474
                     /locus_tag="PLT08_01640"
     CDS             4348..6474
                     /locus_tag="PLT08_01640"
                     /inference="COORDINATES: protein motif:HMM:TIGR01525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HNE03192.1"
                     /translation="MITKQLELRVGNLDCEHDANAIERGLEGFNGLVNLKVYFKSAKV
                     AITYDPAATEPKILKEKLESLGFPPQKGMEMAEQPKPWKNPKVLASAASGILLLIGWL
                     IGLAGAPAILSTMIFIAAILIGGYYFGREAIEELILEREVGIELLMTIAAVVAAAMGE
                     PLEGAMLVFLYSISEAAEGYTEEKTRAAIKALMNLAPKVALVRRENVEREIPVEELEV
                     GDIFIVKPGQSMATDGAVVVGSSSVNQAPVTGESVPVEKQPGDAVFAGSINGEGALEV
                     RATKTFADNTIARIITMVEEAQEKKGKSQRFIERFGKRYSPIVLFFGILIAIVPPLFF
                     GAGWVMWITRATVFIVAAAPCALVISIPITLVASLGTGARNGVLIKGGVYVEELAKVK
                     VVAMDKTGTLTRGEPEVTDVILFRQDPDRLTNSRQDLLAAASGIERRSGHPLAQAIVR
                     YADAQAIQPVELADFRSLTGAGASARLDGRTIYIGSPDLFHSKLSVSLEHAWGDINQL
                     QGEGKTVVIVGDEQDPWGLIAIRDNIRLNAQKAIDAMHAAGVEKVVMLTGDNERTAQA
                     IARELGIDEVYADLKPEDKAVKVRELTSRYGHVAMVGDGVNDAPALAEATVGVAMGAA
                     GTDVALETADVALMADDLEKLAYALRLAKRNQSVVNQNLALSAVVIGVLVIGAVGGWF
                     TLPIAVLAHEISEFVVIGSGLRMLKA"
     gene            6499..>7729
                     /locus_tag="PLT08_01645"
     CDS             6499..>7729
                     /locus_tag="PLT08_01645"
                     /inference="COORDINATES: protein motif:HMM:TIGR01494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HNE03193.1"
                     /translation="MSNTQTLEIPISGMDCAECTMHVQHAIEKLPGVQSVNVFLGTEK
                     AVVKLDPAQVDIPAIRSAVQGAGYDVPASNSPKVDPISMGDFNRKLTVMLVSVFAIIL
                     SVVIFGEVLGLFDFLNERVPFLFGLVLVIAGGYPVFMNVIRATLKRQIISHTLMTLGV
                     IAALVVGQWVTAALVVVFMRIGDYVENFTTESARRAVKELTALTPQTARVERDAKEIE
                     IPVSEVKVGETIIVRPGEKIAVDGEVISGQATVDQSAITGESMPIEAGNGTHVYAATI
                     AKLGSLRIRTERIGSDTTFGRVVKMVEEAEAHRADVQQLADKFSAWYLPVVAVIAALT
                     FIFTRNPLSTAAVLLVACSCSFALATPVAMLASVGASAKRGLLIKGGKYLELLARADV
                     LLVDKTGTLTLGQPQVTD"
BASE COUNT         1810 a         2028 c         2053 g         1838 t
ORIGIN      
        1 gtcgtcatag gtatctcaaa tagttgaata gttgctcaac tatttgagat tgtacacccc
       61 catcatagac tcgtcaagat ggcaatgtaa atccaaatgt tgtcccctga ggcggctgac
      121 tctccaccca taccctgccg ccgtgcgcct ccaccaattg cttgacgata gccagtccaa
      181 ttcccgaacc gccgctggta cgagaacgag atttatcggc gcgatagaag cggtcgaaca
      241 cataaggcag atcgccaggt gaaatcccct cgcctgtgtc agatactgtg ataaccactt
      301 gatggggtgt atcacgctcg caacttattg ttacctgccc gccttcggga gtgtgacgta
      361 gggcattact caacagattc cgtatgactt gtgttatccg atcagtatcc acctcaacct
      421 ggggaagatt ggaagcaaat tcgagattca gttccacttg gctggattgc gcttgtaggt
      481 ggaacggttc aactgcatga gtggtcagtt ctgcaatatt cgtcctgaca cgttccagtt
      541 tcaattgacc cgactcagcc agcgagagca ggcgcaagtc cgcgacgaga cgcgtcaaca
      601 atgcggtctc atcacgaaga gtcgctatct cctgtgggct ggtaggcagg acgccatcga
      661 gcatggcttc aagattgcct tgaatgactg taagaggagt tcgcaattca tgggcaacat
      721 ccgcgatcat gctgcggcgc agctcctcgt gttttgcaag agagtctgcc atctggttga
      781 acgcagttgc caacattccg atctcgtcct gtgactgatt tggaatccgt tgtttcaggt
      841 caccagcggc gattttttgt gcggcggaag tcaacttctg aacaggggat acaatttgga
      901 agaataagat cgagccgagc acgaacgcga ccagggttgc gattcctccc gccaaccagg
      961 tggattgatt gacggatgaa acaaaatctc ccgccgcacc agacacattt gttccagcgc
     1021 tcactggcag gagcgttccc acctgttgat tcccaaccag gatgggtaca cccgttgtga
     1081 ggtctgccga cgaaatggtc ttccccgtat ccagcgcggc actatccgca atgatggttc
     1141 cctgcgtatc ggcaagcagg aggcgtattc ccatcatgtt ccacggattg gagtctccca
     1201 tacccatccc gccgtttgtc cccataccat cccagccctg ccacatgccc caatcccctt
     1261 cgtccatcat ccccatgcca tcccccatca tggaaccacc ccagggatta ttgagcagac
     1321 tttccacacc ctgccagttt ccttgtcggc tgtaatactg ggcaagggtt ggggcaagtt
     1381 gttgcgcgaa cactctgccg ttttgatcaa tgtactgact gaattgagtc cgcgttgaac
     1441 ggctgaccag gtaggtatca atgccgccgc cgatcaatac gatcaccaga aaagctccca
     1501 tcaacttaag ccacaggcta cgaatcatgt cggaactccg cagagaaacg atatcccacg
     1561 ccgaatacag tttgaataaa gcgcggcgca cgggcatcgt cacccagttt ggcgcgcaga
     1621 tttttgatgt gctgatcgat caccctttcg tagccttcat aagcgccacc ttgtgtggct
     1681 tccaccagtt gcaatcgggt gaacacctga cctggatggc gcatcagcgc tgtaaggatg
     1741 tcgaactcag tgggagtcag gtcaaggggt tgattggcat gagtcgcagt atgcgcttca
     1801 aggtccaaaa tgagcgtatc ggcgcgcagg atgggcggtg gctgcacctg tccacgactg
     1861 cgacgcaaca ctactcgaac gcgcgccgtc acttcacgtg gactaaacgg cttggtaacg
     1921 tagtcatcgg caccgagttc caatccgatc aatttatcac tctcctcgct gcgggcggtg
     1981 agcatgatga tgggcgtatc cccctcgcgg cggatctgac ggcagatctc ccagccgtct
     2041 acgtgcggca agtgcaaatc cagaatgacc agatcaggct tttcatgccg aaaggctggc
     2101 attgccagtg caccatcaga gatgatgaca acctggtagc cagcctgttc gagatataac
     2161 cgcagggtgc gggtgatgtg cgcttcgtct tcgacgacta aaatcttgtc tgccaaaacc
     2221 gatctcctgc tatttgtgca tggttaagat ggtgaagcct gcttctgcgt tagtgtgcgc
     2281 acaagccaga aaatgccacc gatgatgagc gcaagaacgc caagtggtac gagccacatg
     2341 aacgccatgc cgagaaaacc aaaaggcatc atgccccatc cacccatcat gggcatataa
     2401 ccgcgattac ccatcatacc gtagccgttc caaccatcac agccgtaacc gattgctgtg
     2461 ttgttccatc caaagggcat gaacataccg ccaaacaagc cgagggcgaa gagagcaatt
     2521 gcgccgatca cgatccagag tgtaacgttt ttcattttat catttccttt cgtaatagat
     2581 ttttcattaa ttattgggag cgcccaaagc aatccaatcc agaatggttt gaatttcggt
     2641 tgaattaagt ggtgtgttac ggaaaggcat gtaaccgctg tacacataat aagcgagtcg
     2701 actgttatat acatcggtgg gaacaacgac cgcgccgttg acactgcctt tcagaacatt
     2761 ctcatatgtg tcgagaaaca atccatttgt cccgccatga caggcaatac aacgcgcggc
     2821 aaagataggt tgaacttcat tcctgaatga gacaatggtg gaaggcgtag gatagtatgg
     2881 cgaagtgttg gggacagtct gcgtgagata caagttggga ttggtctgcc agccgttcat
     2941 catcccgcca ttcatcatac cgccgtgcat accattccaa ttttcataat ccccatcgtg
     3001 catgtcccag ccttgttgtc cttgaggagc gtagttcggg ttcatcatcc accagtctct
     3061 tccccactgt ccctgtggtg gaatgacagg aggcgtagtc tgcggagcgg gtgttacctg
     3121 gagctgtgga agaactattg tcggttgaat ctgctggtgc ggaataaatg tcggctgggc
     3181 aatttgctga gcgggcatgt tcgcagttcg taactgcgtg atgaatgttc ccgccgcaaa
     3241 tccgcccacc agcaaaactc ctgccgcgac caatccgaga atgaatttta aaactttcat
     3301 taggcatctc ctgtttgtga agttacgcac aggatacctg ctagatatat agaagttgtg
     3361 tagaaaaagt aaaagtccta tatatgcctt gacaatctgt ctagaataga taaaatatca
     3421 ttacatactg atacaatgat atgaggaatg tatgattacc gtctctgccc cttctaaaac
     3481 caacttacaa gccaaactct ttcgcggctt cggcgatccc tcgcgcttgg gaattcttga
     3541 tgcgttgcgt aatggaccat taaccgtaag cgaaatcgtc gaggctacca gtctctctca
     3601 gcctaatgtt tcaaatcacc tgggttgttt gcgtgattgt ggtttggtgg tggctgaaca
     3661 acaaggacgg tatgtcacct accatttgag cgacgaccgg gtgggtgaac tgctggctct
     3721 taccgagtca ttgctcgccg atgtcgcgct cggcgtctat gaatgcacgc gctataacat
     3781 ccctcgcaca gagaaataga agaaggcttt gaatgaaacc gatttatcgc gcgctcgtct
     3841 ccctgctgat tgtcttgtcg ttggattgga tgaccaaaac atgggttgaa caagttttga
     3901 tcccctttca gccgatgccg cttgccggac aattcttccg cctgacactt ggctataaca
     3961 caggcgttgc atttgggctg ttcgccaacg gcggagttgg accgctggtt ctcaccggga
     4021 tcattattgt gggcatggtg atctggctgg taagtacgct tcgcagtggc gaattgccgc
     4081 ctacagcggc cgcgcccatc ggactgatcc ttggcggggc aattgccaat tttgccgatc
     4141 gcttaccgga tggccgcgtt accgatttcc tcgatgctgg cttcggtaca actcgttttc
     4201 cgacattcaa cgcggcagat acgttcatcg tgctgggcgt tgccctctta ttgttggtta
     4261 gcttgacgga taaacagcct gtgggaagtg gcggatgaag gtccttagat tcggatatca
     4321 ttttcaatta cttatggaga aattactatg attaccaaac aactcgaact gcgcgtcggc
     4381 aatctcgatt gcgaacatga cgccaatgcc attgagcgcg gcttggaagg atttaatgga
     4441 ttggtgaatt tgaaggtgta ctttaaatcc gccaaggttg caatcacata cgaccccgcc
     4501 gcgaccgaac ctaaaatact gaaagagaaa ctggagtcgc ttggctttcc gccgcagaaa
     4561 ggaatggaaa tggctgagca gcccaagcca tggaaaaacc ccaaagtgct tgcatctgcc
     4621 gcgtcgggca tcctgttgct gatcggctgg ctcatcggac tggctggcgc accggcaatc
     4681 ttatccacta tgatttttat tgccgcgatc ctgatcggcg gatattactt tggacgtgaa
     4741 gccatcgaag aattgatcct cgagcgcgaa gtaggcatcg aactcctgat gaccattgcc
     4801 gcggtcgttg ccgctgcaat gggtgagccg ttggaaggcg ccatgctggt cttcctctat
     4861 tccatcagcg aagccgccga aggatatacg gaagaaaaaa cccgcgccgc gatcaaagcc
     4921 ttgatgaatc tcgcgccaaa agttgcgtta gtccggcgcg agaacgtcga acgtgaaatt
     4981 ccggttgaag aactcgaagt cggtgacatc ttcatcgtca agcccggtca atcgatggca
     5041 accgatggcg cggtggtggt cggatcgtcg agtgttaacc aggcgcccgt caccggcgag
     5101 agcgtgccgg ttgagaaaca gccgggagat gcggtctttg ctggaagtat caacggcgaa
     5161 ggcgctttgg aagtgcgtgc aaccaagacc tttgcggata acaccatcgc ccgcatcatc
     5221 acaatggtcg aggaagccca ggaaaagaaa ggcaagagcc agcgctttat cgagcgtttt
     5281 ggcaagcgtt acagtcccat cgttttattt ttcggcattc tgattgccat tgttccgcct
     5341 ctgttcttcg gcgcgggctg ggtgatgtgg atcacacgcg caacagtctt catcgttgcc
     5401 gccgcaccct gcgctttggt tatttccatc ccgatcacac tagtagcttc gcttggcacc
     5461 ggcgcgcgca atggtgtact gatcaaaggc ggagtgtatg tggaagagct cgccaaggtg
     5521 aaggttgtgg cgatggacaa gaccggcaca ttgacgcgcg gcgagccgga agtgaccgat
     5581 gtgatcctct tccgtcagga cccggaccgc ttaacaaatt ccaggcagga cttattagcc
     5641 gcggcgtctg gcattgagcg gcgcagtggt catcccctgg cacaagctat cgttcgatat
     5701 gcggatgcgc aggctattca accggttgag ttggcagact tccgctctct cacaggcgct
     5761 ggcgcttccg ctcgtttaga tggacgcacc atttatattg gcagccccga tttattccac
     5821 tcgaaactga gcgtctcgct ggagcatgcc tggggtgata tcaaccaact gcagggcgag
     5881 ggcaagaccg tcgttatcgt gggcgatgaa caggatccct ggggattgat tgccatccga
     5941 gacaacatcc gcctcaacgc ccagaaagcc attgatgcaa tgcacgctgc gggagtcgaa
     6001 aaagtggtga tgctgaccgg tgacaatgag cgcaccgccc aggcaatcgc ccgcgaactc
     6061 ggtattgacg aagtgtatgc tgacctaaaa ccggaagata aggctgtcaa agtgcgcgaa
     6121 ctcacctccc gctatggaca tgtggcgatg gtcggggatg gagtcaacga tgcccctgcg
     6181 ctggcggaag cgaccgtagg ggttgcgatg ggagcggcgg gaacggacgt ggctttggaa
     6241 accgccgacg ttgcgctcat ggctgatgat ctggagaaat tagcatacgc gctcagactg
     6301 gcaaaacgca atcaatctgt tgtcaaccag aacctggctc tttcagcggt cgtgatcggc
     6361 gtgctggtca tcggcgcagt gggcggttgg ttcactctgc cgatcgcagt tctggctcat
     6421 gagatcagcg aattcgtggt catcggcagc ggattgcgca tgttgaaagc ataaactact
     6481 cacaaaccag gatttcaaat gtctaacaca caaactctcg aaattcccat ctcaggtatg
     6541 gactgcgccg aatgcaccat gcacgttcaa cacgccatcg aaaaactgcc tggcgtgcaa
     6601 tccgtgaatg tttttctcgg cacagaaaag gcagttgtaa aactcgaccc tgcccaagtg
     6661 gatattcccg cgattcgctc cgctgttcag ggtgcgggat atgacgtgcc cgcttccaac
     6721 tcgccgaaag ttgatcctat ttcaatgggc gatttcaacc gcaaattgac tgtcatgctc
     6781 gttagcgtgt tcgccattat tctcagcgtg gtcatctttg gtgaagtcct gggattgttc
     6841 gacttcctga acgaacgtgt ccccttcctg tttggtctgg tactggtgat tgcaggcggc
     6901 tatcctgtct tcatgaacgt tatccgcgcc acgctcaaac gccagatcat ctcccacacc
     6961 ctaatgacgc tcggtgtcat cgctgcgttg gttgtcggtc aatgggtcac agcggcgtta
     7021 gtggtggtat tcatgcgcat tggtgattac gtggagaact tcaccaccga aagcgcacgc
     7081 cgcgcagtca aggaattgac tgcactcacc ccacaaactg cccgcgtgga acgcgatgct
     7141 aaagaaatag aaatccctgt cagcgaagta aaagttggtg agaccattat cgtgcgtcct
     7201 ggcgaaaaga tcgccgtgga cggtgaagtc atcagcgggc aggcaaccgt ggatcaatct
     7261 gccatcacag gcgaatccat gcccattgaa gcggggaatg gaacccatgt ctatgcggca
     7321 acgattgcca aattgggcag tctgcggatc cgcaccgaac ggattggatc cgatacgact
     7381 ttcggtcgtg tggtcaaaat ggtggaggaa gcggaagcgc atcgcgccga tgtgcagcaa
     7441 ctggcagata aatttagtgc ctggtatctg ccagtggtcg cggttattgc cgcgttgaca
     7501 tttattttca cccgtaatcc actatcaact gctgctgttt tattagttgc ctgttcatgt
     7561 tcctttgcgc tggcgacacc cgttgccatg ctcgcgtcag tgggagcaag tgcgaaacgc
     7621 ggactactca tcaaaggtgg aaaatatctt gagttgcttg cacgcgccga tgtgttattg
     7681 gtagacaaga caggcacact cactctaggt caaccgcagg tgacagatg
//
LOCUS       DAOQJE010000034         7701 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_5172_length_7701_cov_5.641512, whole genome shotgun sequence.
ACCESSION   DAOQJE010000034 DAOQJE010000000
VERSION     DAOQJE010000034.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 7701)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7701)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7701
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5172_length_7701_cov_5.641512"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..814)
                     /locus_tag="PLT08_01650"
     CDS             complement(<1..814)
                     /locus_tag="PLT08_01650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE03194.1"
                     /translation="MSDHVKQLLDLVENSISFDGTTVRVLDAAKFRKNVGKLVERSAL
                     ASDSSAGLARFLVRAAALELGVYAASIHDLYMARGRGDAPMTWSTPAFNLRALSHHAA
                     RAMFRAANKINAGAFIFELARSEMNYTDQRPSEYATNILGAAVAEGYTGPVFIQGDHF
                     QVSAKKYKADAAAELKAVRDLSIEAMAAGFFNIDVDTSTLVDISLPTVQEQQTLNSKL
                     SAELSAFIRENEPAGVTISIGGEIGEVGTENSTEPELRAYMDAYNANLQKLAP"
     gene            995..1516
                     /locus_tag="PLT08_01655"
     CDS             995..1516
                     /locus_tag="PLT08_01655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE03195.1"
                     /translation="MSDFFEKVSSQVDPFKKLVSFIPGFSGYIERQNRRDADKLLRET
                     VARRFEEQWTRASSLQVDMVNNGMLSYVDDMEKASLALRTFIDKISTAARGYSGMFDA
                     VKINEKELEAIYQFDAAFFDLADQVKSGLDNVEASMGDEAALPAAIRNITSLSRLAVE
                     TFERRSEIVTGSR"
     gene            1559..2968
                     /locus_tag="PLT08_01660"
     CDS             1559..2968
                     /locus_tag="PLT08_01660"
                     /inference="COORDINATES: protein motif:HMM:NF013134.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD binding domain-containing protein"
                     /protein_id="HNE03196.1"
                     /translation="MWHNYINATSIDEVVRILAEKRESARVVAGGTDLVLELERGVRK
                     GVDTLIDVTRIPQLDQITIDEDDVIHLGALVTHNDCAASKLIRTRAYPLARASWEVGA
                     PQIRNRGTVAGNLITGSPANDTITPLMALGAKVTLVSDRKTRVVPLKKFYLGVRKTVM
                     EPDEMLVDISFPALTKTQRGTFIKLALRRAQAISLVNAAIILDLKADTVKSASITLGA
                     VTPIITHAPKAEKFLIGKKLNDKNIAAAAELAMKSAKPIDDVRGSANYRREMARVITQ
                     RGLTSIRNGKEQTGMPKKPILLVSEKTTQHGSNIAGSFPENKIETIINGKKYTFTSGH
                     DKTLLRLLREEGMLIGTKEGCAEGECGACTIYLDGQAVMSCLVPAPRAHGAEITTIEG
                     LANGEKLNPVQEKFIEHNAVQCGYCIPGFIMSGAKLLEEKPNPTRNEIEQAITGNLCR
                     CTGYYKIVKAIEEASNSSS"
     gene            3065..3400
                     /locus_tag="PLT08_01665"
     CDS             3065..3400
                     /locus_tag="PLT08_01665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE03197.1"
                     /translation="MMIVLPAISMAAGYELVNLALASGYPVPYQLLGTPRFPDLFYKS
                     SGMMTILSPIIAIRNFYAYAVAAIFIMVLLSGIMSLVYAIVYRMIGPSRYSPLDAPPA
                     NVKAKAYKR"
     gene            3402..5591
                     /locus_tag="PLT08_01670"
     CDS             3402..5591
                     /locus_tag="PLT08_01670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein
                     molybdopterin-binding subunit"
                     /protein_id="HNE03198.1"
                     /translation="MTKKIVGTSIPRIDALGKVIGETEYSGDRHLPGMLYMKTLMAER
                     PHARVREIRIDKALAAPGVVAIYTAKDVPINEYGLQIPDQPVLCGPGSSKPYTDIVRF
                     VGDQVAAIVAETESQAQAAMKLIEVDYEDRPPLTDPVIAMQPDAPLLHPDRGDTNVCV
                     HYRIRKGNVDDAFVKADVIVEGEYQTPVQEHAYLQPEAGLAYIDEDGLIVVECAGQWT
                     HADRKSIAHALDVPEEKVRVIYPAIGGAFGGREDLSVQIVLALAAMKLNRPIKIIWSR
                     RESMIGHGKRHAVLLRAKWGATKDGKVVAVQNEIIGDGGAYMYTTNKVLGNATITCTG
                     PYSVDNVKTDVYGVYTNNVPGAAFRGFGAPQALFMAESQMNKLAEKLGMDPVEFRLKN
                     ALRDGDTLDVGTPAPTPVNIVECIEAARDKYQWKKQGKSKTKKQGAIIKGRGFATGFK
                     NVGFSFGYKENCWAKVEIHGNGSIERVVLHHAGAEVGQGFHTVMTQMAAHVIGVPTKL
                     IEFRASDSATQGNPGSASASRLTFMAGNSVKGAAEAALAKWEAEERPAIAEFSYFAPP
                     TTPMDKETGYSMPNFQYAYVAQAVEVEVDTETGHVRVKRIISADDVGTAINPDLVVAQ
                     IEGAVVQAHGYAMMEEFKTKDGRVLTDQFSTYLLPTIWDIPEKVESVLVEIPDPNGPW
                     GARGVGELPYLPVAAAIAAAIHDATGVWISEFPFTPERVLRALGKIK"
     gene            5639..6430
                     /locus_tag="PLT08_01675"
     CDS             5639..6430
                     /locus_tag="PLT08_01675"
                     /inference="COORDINATES: protein motif:HMM:NF024869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XdhC family protein"
                     /protein_id="HNE03199.1"
                     /translation="MNSIFQALAELEKNKQPAALCTVVKSEGSTPRHVGSKMLVYPDG
                     TFIGTVGGGDLEHRVLDEAWMALSDGQSRHLHYTMSDPSRGDPGVCGGQVEVFVEPIL
                     PPATLVVIGAGHVGKAVVHLAKWLGFHVVVSDDRAEFCTPEATPGADAYYSVEMGKLA
                     ETLKITNRTYLVVTSRGSSVDALGLPSLLESEAAYIGVIGSKRRWATTVKALKENGVK
                     EELIAKVHSPMGLELQAETPEEIAVSIMAEVLLVRDKGTGKSMRA"
     gene            6432..7025
                     /locus_tag="PLT08_01680"
     CDS             6432..7025
                     /locus_tag="PLT08_01680"
                     /inference="COORDINATES: protein motif:HMM:NF024183.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HNE03200.1"
                     /translation="MTLPSNQRMFHGGIRAAALADQLGARFNSRQRRVSVHSQGDTAL
                     VQIGSKHGTPLTVHIADTEGGVLVTMSRERNWLDKAADTSEMMERAANSPLSLLAMIP
                     DIVGEMSQENIAPKVWDAINDLCSLTRSLAGEKNAPRNPKVCTFCGTANDPELELCVA
                     CGGSLPTDLPRVCPKCSRGHTSDALFCQACGTRLVEG"
     gene            7141..>7701
                     /locus_tag="PLT08_01685"
     CDS             7141..>7701
                     /locus_tag="PLT08_01685"
                     /inference="COORDINATES: protein motif:HMM:NF024813.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HNE03201.1"
                     /translation="MARIFDVIEYANEMNDEIVHRFPEEGIGDFRIGSQVIVRESQNA
                     VFFRDGNALDVFKAGRHTIATANIPMLIDFIGKAFNDRTPFPAEVYFVSKKEFANKKW
                     GTSQPILVETPGKGLGYLFIQGFGTFSFQVKDPQQFVTQIVGATGTYRTSEIEERLKT
                     MLVSKLTDVLGEFSQTTQSLAAKISGM"
BASE COUNT         2089 a         1899 c         1890 g         1823 t
ORIGIN      
        1 cgggagccaa tttctgaaga ttggcgttat aagcatccat ataagcgcgg agttcaggtt
       61 cggtggaatt ttcggttccc acttcaccga tttcaccacc aatggagatg gtcacgcctg
      121 caggctcatt ttcgcggatg aaggcagaaa gttctgcaga aagtttgctg ttaagggtct
      181 gctgttcctg aacggtcggg aggctgatat ctacaagagt agaggtatcc acatcaatgt
      241 tgaagaagcc agctgccatt gcttcaatgg aaaggtcgcg aacggctttc aattcagcag
      301 ctgcatcagc cttgtatttc ttggcagaga cttggaaatg gtcaccttga atgaataccg
      361 gtcctgtata tccttctgct acagccgcac ccagaatgtt ggttgcatat tccgacgggc
      421 gctggtcggt gtaattcatt tcagagcgag ccaattcaaa gatgaaagca ccagcgttga
      481 ttttattcgc tgcgcggaac atggcacgcg ccgcatggtg agacaaagcg cgcaagttga
      541 acgctggcgt agaccaagtc atcggggcat cgccacgtcc gcgagccata tagaggtcat
      601 ggatcgatgc agcatagacg cccagttcga gcgccgcagc acgcaccaga aaacgagcca
      661 agcccgcgga ggagtctgaa gcgagcgcag aacgttccac caacttgcca acattcttcc
      721 taaatttagc tgcatccaaa acacggaccg tagttccgtc aaacgaaatg ctattctcga
      781 caaggtcgag taattgtttt acatggtcag acatactatt tctcctaaat aaaaattgat
      841 aacttgatta taactggaat taaacgatat aattgtcccc agtgacgcgc ctgaataaaa
      901 ccggataaat gtcattttag ccccttgttg ccggcaacct tttcagtgtg aaacaaaact
      961 tgaccgagta cgtatatttt ggcaggaggc tcacatgagc gatttttttg aaaaagttag
     1021 tagtcaggtc gacccgttta agaagttggt gtctttcatt cccggtttta gtggttacat
     1081 cgaaagacaa aatcgtcgcg atgcagataa actccttcgc gaaaccgtag cgcgccgttt
     1141 tgaagagcag tggacacgcg cttcaagcct gcaagtggac atggtcaaca atgggatgct
     1201 ctcttatgtt gacgatatgg aaaaagcatc actggcgttg cgtactttta tcgataagat
     1261 cagcaccgct gcgcgcggtt actctggcat gttcgatgct gtgaagatca acgaaaaaga
     1321 actcgaagct atttatcaat ttgatgctgc cttctttgat ctggcagacc aggttaagag
     1381 cgggcttgat aatgtagaag ccagcatggg cgatgaagcc gccctgcccg ccgccattcg
     1441 taacatcacc agtctctcac gccttgcagt ggaaaccttt gaacgccgct ctgaaatcgt
     1501 gacaggcagt cgttaagttt atgtaggggc gggtcacccc gcccctacgg atattcatat
     1561 gtggcacaac tatataaacg caacatccat tgacgaagtc gttcgtatcc ttgctgagaa
     1621 gcgtgaaagt gcccgcgtcg tcgcaggtgg aacagacctt gttcttgaac ttgaacgtgg
     1681 cgttcgcaaa ggcgttgata cactcatcga cgttactcgc attccccaac tagatcaaat
     1741 cacaattgat gaagatgatg ttattcatct tggtgcactg gtcacgcata atgactgtgc
     1801 ggcatccaaa ctcatccgca ccagagccta tccgctggca cgtgcgtctt gggaggtcgg
     1861 cgctccgcaa attcgtaatc gcggaaccgt agcaggcaac ctcatcaccg gctctcccgc
     1921 aaacgatacc attactcctc tcatggcgct tggtgccaaa gttacattgg tctcagatcg
     1981 aaaaacccga gttgttccac tcaagaagtt ttatctcggt gtacgcaaaa ccgttatgga
     2041 acctgatgaa atgctggtgg atatttcctt cccggcattg accaaaactc agcgtgggac
     2101 gttcatcaaa ttggcactgc gccgcgctca agccatctcc ctcgtcaacg ccgcgatcat
     2161 tctggacctc aaagcggata ccgttaaatc tgcctcgatc acactgggag ccgttacacc
     2221 catcattacc catgcaccaa aagctgaaaa attcctcatt gggaaaaaac tcaacgacaa
     2281 aaatatagct gccgctgcag aactagcgat gaagtctgcc aagccgattg atgacgtgcg
     2341 cggctctgct aactatcgcc gcgaaatggc acgtgtcatc acccaacgtg ggctgacatc
     2401 cattcgcaac ggcaaagaac aaacaggtat gcccaaaaag ccaatcttac tggtgagcga
     2461 aaaaacaact caacatggct ctaacattgc aggttctttc cctgaaaata agatcgaaac
     2521 gattatcaat ggcaagaaat acacgttcac aagcgggcac gacaagaccc tgttacgttt
     2581 gctacgcgaa gaaggaatgt tgatcggcac caaagaaggc tgcgccgagg gcgaatgcgg
     2641 ggcatgtact atctatttag acgggcaagc cgtcatgtct tgccttgtcc ctgccccacg
     2701 ggcacatggc gcagaaatta ccaccatcga aggtttggca aatggagaga aactaaatcc
     2761 ggttcaagaa aagtttattg agcacaatgc cgttcaatgc gggtactgca tccccggctt
     2821 tatcatgtct ggtgcgaaac ttcttgaaga aaaaccaaac ccaactcgca acgagatcga
     2881 acaagccatt accggcaatc tctgccgttg taccggatat tacaaaatag taaaagccat
     2941 tgaagaagca agtaattcat cgtcttaaga ggtatgaatg agtaaatacg gtggttcgtc
     3001 cagaaaagta ataaaagaaa gaaataaaca gccacattat ctttggcgag gtattggctg
     3061 tcttatgatg attgtcctcc ctgccatttc catggcagcc gggtatgaac tcgtcaacct
     3121 cgctcttgca agcgggtatc cagttccgta tcaactgctt ggcactccaa gatttccgga
     3181 ccttttttac aaatccagcg gcatgatgac cattctcagc cccatcattg ctattcggaa
     3241 tttttatgcc tacgctgtag ccgctatttt cattatggtt ctgctgagcg gtattatgtc
     3301 gctcgtgtat gctattgtgt accgcatgat tggtccatca cgctatagcc ctctcgatgc
     3361 tcctccagca aacgtcaaag caaaagccta taaaagataa aatgacaaaa aaaatcgttg
     3421 gaacctccat ccctcgtatt gatgcactcg gtaaggtgat cggcgaaact gaatactccg
     3481 gtgaccgtca cctgccgggt atgctttaca tgaaaaccct catggcagag cgccctcatg
     3541 cccgcgtgcg ggaaattcgc attgataaag cccttgctgc gccgggcgtt gttgccatat
     3601 acacagcaaa agatgttcca atcaacgagt atgggctcca aatcccagac cagcccgtcc
     3661 tttgcggacc tggttccagc aagccctaca cagatatcgt ccgctttgta ggagatcaag
     3721 tcgcagccat cgtagcagaa acagagtctc aagctcaagc agccatgaaa ctcatagagg
     3781 tggattatga agaccgaccg cctcttacgg acccggtcat tgctatgcag cccgacgccc
     3841 ctctcctcca cccagaccgg ggtgacacca atgtatgtgt ccattatcga atccgcaaag
     3901 ggaatgtgga cgatgccttt gtgaaagccg atgtgatcgt tgaaggcgag tatcaaactc
     3961 cggtgcaaga acatgcctat ctccaacctg aagcgggatt ggcatacata gacgaagatg
     4021 ggctcattgt cgtcgagtgt gctggtcagt ggacacacgc tgaccgtaaa tccattgcac
     4081 atgcattgga tgtgcctgag gaaaaagtcc gcgtaattta ccctgcgatc ggcggcgcat
     4141 ttggagggcg tgaagacctt tcagtgcaaa tcgtgcttgc ccttgcagcc atgaagctaa
     4201 atcgcccaat aaaaatcatc tggtcacgcc gcgagtcgat gatcgggcac ggaaaacgtc
     4261 atgccgtttt gttgcgcgcc aaatggggcg caacaaaaga cggcaaagtt gttgctgttc
     4321 aaaatgaaat tatcggggat ggtggtgcat atatgtacac caccaataaa gtcttaggaa
     4381 acgccaccat tacctgtacg ggtccttact ctgttgataa cgtaaaaacc gatgtttacg
     4441 gtgtgtatac aaacaacgtc cctggagcgg cttttcgcgg gtttggcgcg cctcaagcgt
     4501 tattcatggc agagagccaa atgaataaac tcgctgagaa acttggcatg gacccggttg
     4561 agttccgttt gaagaatgct ttacgcgatg gcgatacttt agatgttggc actccagctc
     4621 cgaccccggt caatattgta gagtgcattg aagcggctcg tgataaatac caatggaaga
     4681 aacaagggaa aagcaaaaca aagaagcagg gcgctattat caaagggcgc ggttttgcaa
     4741 ccggcttcaa gaatgtaggt ttctcttttg gttataaaga gaattgttgg gcaaaagttg
     4801 aaattcatgg caatggcagc attgagcgcg ttgtactaca tcacgctggt gccgaagttg
     4861 gtcagggttt ccatactgtc atgacccaaa tggcagcaca cgtaatcggc gtaccaacca
     4921 agcttattga atttcgcgcc tccgactctg caacacaagg caaccccggc tcggcttcag
     4981 cttcacgcct cacttttatg gctgggaatt ctgtgaaagg agcggcagag gctgccctag
     5041 caaaatggga agcggaagaa cgccccgcca ttgcagaatt ttcatacttt gcaccgccca
     5101 ccactcccat ggataaagaa accggctatt cgatgccgaa cttccaatat gcgtatgtgg
     5161 cacaggctgt ggaagtggaa gtagataccg aaactggtca tgtgcgtgtc aaacgcatca
     5221 tttctgcaga tgatgttggt actgctatca atcccgatct cgtggtggca caaatcgaag
     5281 gagcggttgt tcaagcacat ggctatgcca tgatggaaga attcaagacc aaagatgggc
     5341 gtgttctaac agatcagttc agtacctatc tattgcctac aatttgggat attcctgaaa
     5401 aagttgaatc tgtcctagtt gagattccag acccgaacgg accttggggt gcacgtggtg
     5461 ttggagaatt accttatttg ccagttgccg ccgctattgc cgctgccatc catgatgcaa
     5521 caggggtctg gattagtgaa tttccattca ccccagaacg agtgttacgg gcattaggga
     5581 aaataaaata aaaaacaaag gactgacacc aaaacctgtc agtcctttat tggatttcat
     5641 gaattcaatt ttccaagctt tagccgaact tgaaaagaat aagcaacctg ccgctctttg
     5701 tactgtggtc aagagtgaag ggtctacgcc gcgccatgtg ggcagtaaaa tgctggttta
     5761 cccagatggt acatttattg gcacagtagg cggtggagac ttggaacatc gcgtcctcga
     5821 tgaagcttgg atggcgttaa gcgatggaca atcgcgccat ttacattaca ccatgtctga
     5881 cccttcgcgc ggcgacccag gcgtatgtgg aggtcaggtg gaggtatttg tggaaccgat
     5941 tcttcccccc gccaccttgg tagtgatcgg tgcagggcat gttggcaaag ccgttgtcca
     6001 ccttgcgaag tggctgggct tccatgtcgt tgtcagcgat gaccgtgccg agttttgcac
     6061 cccagaggct acccccggtg cagatgcata ttactctgtc gaaatgggga aattggcaga
     6121 gacactcaaa atcacgaatc gaacttattt ggttgtgacc agccgcgggt ccagtgtaga
     6181 tgcgttgggt ctgccatccc tacttgaaag tgaagcagcc tatatcggtg tcatcgggtc
     6241 aaaacgcagg tgggcaacca cggtcaaggc attgaaagaa aacggtgtga aggaagaatt
     6301 gatcgccaaa gtccattcac ccatgggctt ggaattacag gcagaaacgc ccgaagagat
     6361 cgctgttagt attatggctg aagtgttact tgttcgagac aaaggcactg gcaaatcaat
     6421 gagggcataa gatgacatta ccatccaatc aacgcatgtt ccatggagga atacgtgcgg
     6481 cagcattagc agaccaactc ggtgcacgtt tcaacagcag acaaagacgc gttagtgtac
     6541 attcacaagg cgatacggct ttggtacaaa tcggatccaa gcatggaact ccgcttacgg
     6601 ttcatattgc tgatacagaa ggcggtgtat tagtgaccat gagtcgcgaa cgaaactggc
     6661 tcgacaaagc cgcagatacc agtgaaatga tggaacgggc ggcgaacagt ccgctttcgc
     6721 ttttagcaat gattcccgat attgttggcg aaatgagcca agaaaatatc gccccaaagg
     6781 tttgggacgc aatcaacgat ctatgttcgc tcacccgctc acttgcaggc gaaaagaacg
     6841 ctccacgcaa cccaaaggta tgtactttct gcggaaccgc caacgacccc gaacttgagt
     6901 tgtgcgtggc atgcggcggc tccctcccca ccgacttgcc tcgggtatgc ccaaaatgca
     6961 gccggggaca tacatcagat gccctattct gccaagcatg tggaacacgc ctcgtagaag
     7021 ggtaattctg caacgaaacc cgcaatttcg cgtacataag cacatatagg aaaaacggtt
     7081 cttgaccgaa aaatcagatg gactataatc aaaacgttat ttactcaaag gagagaaacc
     7141 atggctagaa tttttgacgt cattgaatat gcaaatgaaa tgaacgacga gatcgtacac
     7201 cgcttcccag aagaagggat tggtgatttc cgcatcggct cgcaggtaat cgtgcgcgaa
     7261 agccaaaacg ccgttttctt ccgcgacgga aacgctctgg acgtgttcaa ggctggtcgc
     7321 cataccattg cgactgccaa catcccgatg ttgatcgact tcattggcaa ggcgtttaat
     7381 gaccgcaccc ccttccccgc cgaagtttac ttcgtctcaa agaaggaatt cgcaaataag
     7441 aaatggggca cctcacaacc catcctcgtg gaaactccgg gcaaaggctt gggctactta
     7501 ttcattcaag gctttggtac tttctccttc caggtgaaag acccgcaaca gtttgtgact
     7561 cagattgtgg gcgccactgg aacctatcgt acatctgaaa tcgaagaacg ccttaagacc
     7621 atgttggtat ccaaattgac cgatgtgctc ggtgaattta gtcagaccac ccaaagcctc
     7681 gctgccaaga ttagcggtat g
//
LOCUS       DAOQJE010000035         7637 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_5235_length_7637_cov_5.525983, whole genome shotgun sequence.
ACCESSION   DAOQJE010000035 DAOQJE010000000
VERSION     DAOQJE010000035.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 7637)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7637)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7637
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5235_length_7637_cov_5.525983"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..447
                     /locus_tag="PLT08_01690"
     CDS             <1..447
                     /locus_tag="PLT08_01690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE03202.1"
                     /translation="EIVVAAATLLSMNWLSIHLISTDDHIRPELLTALNRFGNKPGLY
                     YQTQPSQFVERLTTMKHEIFSKDFFWLAMDKEGEIVASKILEENGQTPTKKVYQSVHK
                     LNLMEPTITPPPLTPDEKSYMEWCASLYESLTPWQNIIKNIQSDLS"
     gene            634..1914
                     /locus_tag="PLT08_01695"
     CDS             634..1914
                     /locus_tag="PLT08_01695"
                     /inference="COORDINATES: protein motif:HMM:NF012427.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNE03203.1"
                     /translation="MRIVIIAPGSRGDVQPYIALGKGLQNAGHYIRLVSHSNFETLVT
                     SYGLEFWSFGNDVKDAVENSEMRELTEKGNFLLLMAKMAKEAEREALRFAEGGLLASQ
                     GMDVVLAGIGGLFIGTAIAEKLHMPLVQAYVFPFTPTKDFSSVLTPKLPTVFNRLSHH
                     LARQLMWQGFRSADGIARKEALNIPSAPFLGPYDSRSTNNMPVLYGFSPSVISTPSDW
                     NGNTHITGYWFIDETNDWQPPVALLDFLKSGPPPLYIGFGSMSNRNPEQTADLVIQAL
                     AMTNQRAILLSGWSGLQKTNVPDSILMVDSIPHSWLFPRVSAVIHHGGASTVAAGLRA
                     GVPSIIIPFFGDQPFWGQRIADLGVGTKPIPRKKLTAEKLANAIREVVTNQEMRQRAE
                     KLGKQIQSENGIKSAVEIFNKLEQQLPNKASKGN"
     gene            1951..2340
                     /locus_tag="PLT08_01700"
     CDS             1951..2340
                     /locus_tag="PLT08_01700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE03204.1"
                     /translation="MKTNKAFFTLVTITSIICLCIGCFFAFSMPVYKTLDNPTAEEGQ
                     QKANELIIALEQYKNDTGSYPSELGSLVPDYLSAIPQPTWSAHYSYELQPNEDEFIIS
                     FDVGISFDGDYCEYNSRTKNWHCSDKI"
     gene            2432..2893
                     /locus_tag="PLT08_01705"
     CDS             2432..2893
                     /locus_tag="PLT08_01705"
                     /inference="COORDINATES: protein motif:HMM:NF024698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNE03205.1"
                     /translation="MLTIDFLEDHLDTIPTLVKWFRNQWTEYYANSSDEAMQQDFLED
                     ILRDRLPSRLVAFEGNELVGTIVLREGDAELSPEHRPELGGLYVAESHRGHGIGTELI
                     KAGMKLAQDQGYSEISATTVKAAGILEGLGWKFIKKIQYPDGEVSLYHCKI"
     gene            complement(2957..4462)
                     /gene="malQ"
                     /locus_tag="PLT08_01710"
     CDS             complement(2957..4462)
                     /gene="malQ"
                     /locus_tag="PLT08_01710"
                     /EC_number="2.4.1.25"
                     /inference="COORDINATES: protein motif:HMM:NF011080.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004134 - 4-alpha-glucanotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0005980 - glycogen catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HNE03206.1"
                     /translation="MAFKRSSGILLHPTSLPGPYGIGDLGPQAFRFVDWLSSTGCKIW
                     QILPLGPTGYGDSPYQCFSAFAGNPYLISPDDLLAENLVTEEDLVELKELPASKVDFG
                     LFIPKKLELLAKAFQRYQADPEPLREAFNYFCAENAYWLDDFALFMALKEANGGGAWN
                     GWDPALRARKKTALNKAKKEQAENVERFSFYQFLFFRQWTRLRKYANEKGIQIMGDIP
                     IFVAYDSADVWANPDLFFLDEAGNPTVVAGVPPDLFSTTGQLWGNPLYNWKVHKKDGY
                     AWWLSRVRALLQTVDILRFDHFRGFAGYYEIPASHTTAEHGRWVPGPAKDFFRAVDED
                     LGNGVIAPGTGLPIVAEDLGVITPDVIELLNAFDLPGMKVLQFAFTGPENPFLPHNYV
                     PNCVAYTGTHDNNTAKGWYADAPEAEKDFARRYLAVDGHDFSWDLIRAVWRSVAVFAI
                     APMQDVLGLGGEARMNFPSRLGGNWEWRMSESDFREDLAAGLRDLNWMTLR"
     gene            complement(4465..>5139)
                     /locus_tag="PLT08_01715"
     CDS             complement(4465..>5139)
                     /locus_tag="PLT08_01715"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNE03207.1"
                     /translation="NGIDTVSFDPETDKSISVNYTLETLDRRVENKELLQEKLGLKRD
                     PNVPLLAIISRMDVQKGVDLAFTALKNMKRIDFQAVILGTGDPKLEEAARELQNLYPD
                     KIKAELRFDAALARQIYAGADMILMPSRYEPCGLAQMIAMRYGCIPVVRSVGGLKDTV
                     IPNETGFIFEKAHHMSLVSAIKAALKIYPDREKWAALQRNGMAKDFSWEASAREYLKL
                     YQSLVK"
     assembly_gap    5140..5239
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<5240..6041)
                     /locus_tag="PLT08_01720"
     CDS             complement(<5240..6041)
                     /locus_tag="PLT08_01720"
                     /inference="COORDINATES: protein motif:HMM:NF019924.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen/starch synthase"
                     /protein_id="HNE03208.1"
                     /translation="MSRTIHVLFLAAEADPFIKVGGLGDVSGVLPRELRALSDDELKL
                     DVRLVLPYHPAVKAENLRPIEIFSIPRGDSEVTVEAFETTFNGMPVYFIGGEPIHSSG
                     SVYSLDAKLDAEKYAFFSLAAMELPKHINWQPDVIHANDWHTALSLYVNLTKRWEAGA
                     KHIANVLTLHNMPFMGADVRAILEQYRIQLAQTDLPEWARVLPLPLGLWASDAIVAVS
                     PGYAGEVLTEEFGCGLNEFMSLRKETLSGILNGIDTVSFDPETDKSISV"
     gene            complement(6069..6872)
                     /locus_tag="PLT08_01725"
     CDS             complement(6069..6872)
                     /locus_tag="PLT08_01725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 2 family protein"
                     /protein_id="HNE03209.1"
                     /translation="MKHHLVEPGKKIKLAEVDPQDTGKFKGGKEEGLKEIAKLNEKLQ
                     ELQELLYAEGKHKVLVVLQAMDTGGKDGAIRRVFDGVNPQGVKVASFKVPTSEEMAHD
                     YLWRVHKVVPASGELVVFNRSHYEDVLVVRVHNYVPKEVWSKRYEQINAFEKLLTENG
                     TTILKFFLHISKDEQKERLQARLDDPSKHWKFSVGDLGERKLWDEYQAAYEDALNKTS
                     TEHAPWYVVPANRKWYRDLVISTVLVDTLKGLKMKYPEPKDDLSGVVIE"
     gene            complement(6956..7030)
                     /locus_tag="PLT08_01730"
     tRNA            complement(6956..7030)
                     /locus_tag="PLT08_01730"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6996..6998),aa:His,seq:gtg)
     gene            complement(7061..7528)
                     /locus_tag="PLT08_01735"
     CDS             complement(7061..7528)
                     /locus_tag="PLT08_01735"
                     /inference="COORDINATES: protein motif:HMM:NF025054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNE03210.1"
                     /translation="MPEIRPVTKDNWKELVALKVRDDQKHFVASNLYSIAESQFGETI
                     PGEGHWDMFPYGMYDEGTPVGFLMLSYNYEFARFQGFILRLMVDDAHQGKGYGKFGMN
                     WMLEKYRADGRVRVVGISYEPNNEVARTLYANLGFQETGEIVDGEALALLNLR"
BASE COUNT         2155 a         1814 c         1615 g         1953 t
ORIGIN      
        1 gaaattgtcg tcgcagcagc tacactcttg tctatgaatt ggctgtctat tcacttgata
       61 agcacagatg accatataag acctgaactc ctcactgcac tgaataggtt cggaaacaag
      121 ccaggtcttt actatcagac tcaaccaagt caatttgttg aaagattaac gaccatgaag
      181 cacgagattt tttcaaaaga tttcttttgg cttgccatgg ataaagaagg ggaaatcgta
      241 gccagcaaaa tacttgagga aaatggacaa acgcctacaa agaaggtgta tcagtccgtc
      301 cataaactga atttaatgga accaacgata acacccccac cactaacacc tgacgagaaa
      361 tcttatatgg aatggtgtgc ttcgctttat gaaagcctga ccccttggca aaatatcatc
      421 aaaaatattc aatcggattt gtcgtgatga ataaagccaa acggataaaa tagtagccta
      481 attacacaaa gcgcccaaca aagcgcgagc tcacagccca agcagtttgc tacgactgag
      541 cctttttctg gttggacggc ttcaccataa cgtaatgcac ttcagtagct accgtaacaa
      601 aaatattctt gataagagga taattcatac acgatgcgta tcgtaatcat tgcaccagga
      661 agtcgcgggg atgttcaacc ttatattgca ttaggaaaag gcttgcaaaa tgcaggacat
      721 tacattcgtc ttgtaagcca tagcaatttc gagacattgg ttacatcata cgggcttgaa
      781 ttttggtctt ttggaaatga tgtaaaagat gccgttgaaa atagtgaaat gcgagaattg
      841 accgagaagg gaaattttct tttgctaatg gcaaaaatgg caaaagaagc cgagcgtgaa
      901 gcactccgtt ttgccgaagg tggtttacta gcctcacaag gaatggatgt tgtgttggca
      961 ggcataggag ggttattcat tggcactgca attgcagaaa aactacatat gccgctcgtt
     1021 caagcctacg tttttccttt cacacccacg aaagattttt ccagtgttct tacgccaaag
     1081 ctgccaacag tattcaatcg tttgtcacat catcttgccc gtcaactaat gtggcagggg
     1141 tttcggtcag cagatggcat agcaagaaaa gaagcgttga acataccatc ggctccattt
     1201 ctaggacctt acgattcaag atcgacaaat aacatgcctg ttctttatgg ttttagtcct
     1261 tcagtaattt ctacaccatc agattggaat ggcaatactc acattactgg ctattggttt
     1321 atagatgaaa caaatgattg gcaaccgcct gttgctctgc tggatttctt gaaatcgggt
     1381 cctccgccac tgtatattgg gtttggcagt atgagcaatc gcaaccctga acaaacagcc
     1441 gatttagtta tccaagcatt agcaatgaca aatcaacgag caatcttgct ttcaggttgg
     1501 agcggtctac aaaagacaaa tgtgccagac tcgatattaa tggtggactc tattccgcat
     1561 tcatggctgt ttccgcgtgt gtccgctgtt attcatcatg gcggggcgag cacagtagct
     1621 gcgggtctca gggctggagt cccatccatt attattccct tttttggaga ccaaccattt
     1681 tggggtcaac ggattgctga tttaggagta gggacaaaac cgattccgcg aaagaaactt
     1741 acggctgaaa agctcgcaaa tgctattcga gaagtagtga ctaatcaaga aatgcgtcaa
     1801 cgtgctgaaa agttaggaaa acaaattcaa tccgagaatg gaataaaatc tgctgtagaa
     1861 atatttaata aacttgaaca gcaattgccg aacaaagcat ccaaagggaa ttaggataac
     1921 gtcccagcag gatgacatat tgcaaaccta atgaagacaa ataaagcttt tttcactctt
     1981 gtaacaatta catctattat atgtttatgt attggatgtt tttttgcctt ttcgatgcca
     2041 gtgtataaaa cactcgataa ccctacagcc gaagaagggc aacaaaaggc taacgagttg
     2101 ataatagcct tggaacaata caaaaatgat acaggaagtt atccctcaga attaggttca
     2161 cttgttccag attatttatc tgccatacca cagcctacat ggagtgccca ttattcttat
     2221 gaacttcaac ccaatgagga tgagttcatt atctcctttg atgtgggtat aagtttcgac
     2281 ggggattatt gcgaatataa tagtcggaca aaaaactggc attgtagcga taaaatttga
     2341 tagttctaac cagtaaaatc agagcatata caagatcgtt tctcaaaccc taaaaacgaa
     2401 aagtatctgc tttaagaaag cagaaaaaaa tatgcttaca attgacttcc ttgaagatca
     2461 tcttgatacc atacctacat tggtaaagtg gtttcgtaac caatggacag agtattatgc
     2521 caattcgtca gacgaagcca tgcagcaaga ttttctcgaa gatattttga gagaccgctt
     2581 accctctcga ctcgttgcat ttgaaggcaa tgaacttgtt ggtacgattg tactgcgaga
     2641 aggcgatgct gaattatcgc ctgaacatcg tcctgaatta ggaggtttgt atgttgcaga
     2701 gtctcatcgc ggtcacggca ttggtacaga attgattaaa gcaggtatga aattagctca
     2761 agaccagggt tattcagaaa tttctgcaac aacggttaaa gcagcaggca tcttagaggg
     2821 tttaggttgg aagttcatca agaaaataca atatccagac ggggaagttt cattgtatca
     2881 ttgtaaaatc taagataatt caaaaaagca tctcacctca ataaaaagaa accgcctcat
     2941 acgaggcggt ttctttttat ctcaaggtca tccagttgag gtctcgcaga cctgcggcaa
     3001 ggtcttcacg gaagtcgctt tcagacatgc gccactccca gttaccaccg aggcggcttg
     3061 gaaagttcat gcgggcttct ccgcccaagc caagcacatc ttgcatgggg gcaatagcaa
     3121 agactgccac agaccgccac acggcgcgga tcaaatccca cgagaagtcg tgtccatcca
     3181 ccgcaaggta gcggcgagca aaatcttttt ctgcctcagg agcatcagca taccaacctt
     3241 tggcagtatt gttatcgtga gtgcccgtgt aagcaacaca attcgggaca tagttatgcg
     3301 gcaagaaggg attctctgga cccgtaaaag cgaattgcaa aactttcata ccaggcaaat
     3361 caaaagcgtt caacaattcg atcacatctg gagtgatcac gcccaaatct tcggcaacaa
     3421 ttggcaaacc tgttcctggg gcaatcacgc cattcccaag atcctcatcc actgcgcgga
     3481 aaaagtcttt ggcaggaccc ggcacccaac gcccatgttc ggcagtggta tgcgaagctg
     3541 gaatttcata ataccctgca aaaccgcgga agtgatcaaa acgcaaaata tctaccgttt
     3601 gcaacaaagc gcggactctg gaaagccacc aagcataacc atcttttttg tgaaccttcc
     3661 agttataaag cgggttgccc cacaattgac cagtcgttga gaaaagatca ggtggaaccc
     3721 ctgccaccac agtgggatta cccgcctcat caaggaagaa caaatctggg tttgcccaaa
     3781 catctgccga gtcgtaggcg acgaagatgg gaatgtcgcc catgatctga attccctttt
     3841 catttgcgta cttgcgtagg cgagtccact ggcgaaagaa aaggaattga tagaaagaaa
     3901 aacgctctac attttcagct tgttcttttt tcgctttatt caaagccgtc tttttacgag
     3961 cacgcaaggc ggggtcccaa ccgttccaag cgccaccacc gttagcttct ttcaatgcca
     4021 tgaagagggc aaagtcgtcg agccagtagg cgttttcggc gcagaaatag ttgaaggctt
     4081 cacgcaaagg ttcggggtcg gcttggtagc gctgaaacgc tttcgccaaa agctcaagct
     4141 ttttagggat gaacaagcca aagtccactt ttgaggcggg aagttcttta agttcaacaa
     4201 ggtcttcttc agtcaccaag ttttcggcaa gtaaatcatc tgggctaatg aggtaagggt
     4261 ttcctgcaaa ggcagaaaaa cattgatacg gcgaatcgcc ataccctgta ggacccaacg
     4321 gcaaaatttg ccagatcttg caacctgtag atgaaagcca atcgacaaaa cgaaatgcct
     4381 gcggaccaag gtcgccgatg ccatatggac cgggcaaact ggtaggatga agaagaatgc
     4441 cggaagaacg tttgaatgcc atgtttattt caccaacgat tgatagagtt ttaggtattc
     4501 gcgtgctgaa gcttcccatg aaaaatcttt tgccatgcca ttgcgctgca aggctgccca
     4561 cttttcacgg tctgggtaaa ttttcaatgc ggctttgatg gcactgacca atgacatatg
     4621 atgtgccttt tcaaagatga agcccgtttc atttgggatg accgtatctt ttagcccgcc
     4681 cacagatcgc acgaccggaa tacacccata gcgcatggcg atcatttgtg cgagaccaca
     4741 cggctcataa cgagacggca tgaggatcat atcggcgccg gcgtagattt gacgggcaag
     4801 tgccgcatcg aatcttaact cggctttgat cttatcgggg taaagatttt gcaactctcg
     4861 agccgcttct tccaacttcg ggtcgccggt tccaagaatc acagcttgga agtcgatgcg
     4921 cttcatattc tttagcgcag tgaaggcaag gtctacgcct ttttgaacat ccatacgtga
     4981 aatgatggca agcaacggaa cattcgggtc acgcttgagc cctagtttct cttgcagaag
     5041 ttctttattc tcaactcggc ggtcgagtgt ttcgagggtg taattgacac taatggattt
     5101 atcggtttct gggtcaaaag aaactgtatc aatgccattn nnnnnnnnnn nnnnnnnnnn
     5161 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5221 nnnnnnnnnn nnnnnnnnnt gacactaatg gatttatcgg tttctgggtc aaaagaaact
     5281 gtatcaatgc catttaaaat accgctcaag gtttctttgc gcaaagacat gaattcgttc
     5341 aagccacatc cgaattcttc tgtgagcact tcccccgcat accccggtga gacggcgaca
     5401 atcgcatctg atgcccaaag ccctaagggc aagggcaaaa cacgcgccca ttcgggcaaa
     5461 tcggtttgag ccaattgaat gcgatactgc tcaagaatag cacggacatc cgcccccatg
     5521 aaaggcatat tatgcagggt aagcacattg gcaatatgtt ttgcaccggc ttcccagcgt
     5581 ttggtcaaat tgacatataa actcagtgcg gtatgccagt cgttggcatg aataacatct
     5641 ggctgccagt tgatgtgctt ggggagttcc atcgccgcca gtgagaagaa agcatacttc
     5701 tcagcatcca attttgcgtc taaggaatac accgagccgc tggaatggat cggttcaccg
     5761 ccaataaaat agaccggcat tccattaaat gtagtttcaa acgcttcgac agtaacttct
     5821 gaatccccgc gcggaatgga aaaaatttcg atagggcgca aattttccgc tttcaccgct
     5881 gggtgatacg gcaaaaccaa cctcacatct aatttcaatt cgtcatctga aagagcgcgc
     5941 aactctcgcg gcagcacccc tgaaacatcg cccagcccgc caactttgat aaaagggtcg
     6001 gcttcggcag cgagaaaaag cacatggatt gttctagaca tgagcgctat tttacaccct
     6061 ttacagactt actcgatcac aaccccgctc aaatcatctt tcggctcggg gtacttcatt
     6121 tttaagcctt tgagcgtatc caccaacact gtggaaatca ccaaatcgcg ataccatttg
     6181 cggttggcag gcaccacata ccacggggca tgttcggtgc tggtcttatt caatgcatct
     6241 tcataagccg cctgatactc atcccacaat ttgcgctcgc caaggtcacc aacgctgaac
     6301 ttccaatgct tggatgggtc atctaagcgt gcttgcaggc gttctttctg ctcatcttta
     6361 ctaatatgaa ggaagaactt taggatagtc gtgccgtttt ctgtcagaag cttctcaaaa
     6421 gcgttgatct gctcataacg cttcgaccaa acttcctttg gcacgtagtt atgtacacgc
     6481 accactaaaa catcttcata gtggctgcgg ttgaacacca ccaattctcc actggcaggc
     6541 actactttat gcacacgcca taaataatca tgcgccattt cttctgaggt cggcacttta
     6601 aagcttgcca ccttcacccc ctgcgggttg acgccgtcaa atacccgccg aatggcacca
     6661 tctttcccgc cggtgtccat tgcctgtaac accaccaaga ccttatgctt cccttcagca
     6721 taaagtaact cctgcaattc ttgcagtttt tcatttaatt tcgcaatttc tttaagtcct
     6781 tcttccttac cgcccttaaa cttccccgta tcctgcgggt ctacctctgc taatttgatc
     6841 tttttacccg gttcaaccaa atgatgtttc atgagacctc cgatattaaa acctttattg
     6901 cacaggtata tatcatatta aaacaaaaag acctagatta aggaccaggt ctttttgggg
     6961 cggatgatgg gtttcgaacc cacaaccctc acagccacag tgtgatgctc tgccattgag
     7021 ctacatccgc catataaagc ggacaaaatt ttaccacaat tcagcgcagg ttcaagagtg
     7081 ccaacgcctc accatctacg atctcgccag tctcttgaaa ccctaagttt gcatataatg
     7141 tacgcgccac ttcattgttc ggctcataac taatccccac aacccgcaca cgcccgtccg
     7201 cgcgatattt ttcgagcatc cagttcatac cgaatttgcc ataccccttg ccctgatgcg
     7261 catcgtccac catcaaccgc aggataaaac cctgaaacct cgcaaactca tagttgtagc
     7321 tgagcatcaa aaaaccaacc ggcgttcctt catcgtacat cccatatgga aacatatccc
     7381 agtgcccctc gccaggaatc gtctcgccaa attgagattc agcaatggaa tacaaatttg
     7441 acgccacaaa atgtttttgg tcatcccgaa ctttcaaggc gaccaactct ttccaattgt
     7501 cttttgtgac ggggcgaatc tcaggcatgt ttcagaacgc tttggatcga atcaagaacc
     7561 gcttctttct tgacctgaac ctgctcgcca ctcacaagat ttttgacgac caccaagcct
     7621 tgcgccgcct catctgg
//
LOCUS       DAOQJE010000036         7598 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_5279_length_7598_cov_5.908922, whole genome shotgun sequence.
ACCESSION   DAOQJE010000036 DAOQJE010000000
VERSION     DAOQJE010000036.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 7598)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7598)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7598
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5279_length_7598_cov_5.908922"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2790
                     /locus_tag="PLT08_01740"
     CDS             <1..2790
                     /locus_tag="PLT08_01740"
                     /inference="COORDINATES: protein motif:HMM:NF024298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="WD40 repeat domain-containing protein"
                     /protein_id="HNE03211.1"
                     /translation="CRKELEIAIEYNKRLFPIIHQNIDPQSSPAALASLNWIFFREAD
                     DFKGAMQKLMAAINTDLDWVKQHTVLLKRANEWNSKKQDGSYLLRGSELQGAELWLSK
                     SSDNKQPHPTPLQSEYIFASRQDASRRQRNTLIGVSAALVVSILLAIAAIVGGVEAVR
                     KSQQALASQLAAQSITLVDTQPDLSLLLSLESNYIGDTLGDADPASLGSLVSSINSVP
                     QLDTYLRAHAGEIREVTFSSNGRWVAATGNAINDQGVVTVWDLASKENPIADQQFIGG
                     TQRILGLAFSPDSKRIAAAGDEKKIFIWDPQTCCETVKEFEIDNKARSLKFVTLNGKE
                     YIAIASGQQITFWDSSTGKKDEALTLQISTTDETVRILSLAVAPTNDMVAAGSEDGNI
                     TVWELKTKEVKFQACSFGDPETNNEEICKVNGDGETDIRGLAFNANGTLLASGSSDHR
                     VWLWNPETGDLLARSAVSTEGGHINTVTSVTFNSKTGTIASVSWDNTVRIWEPIQNED
                     KTWGLELVESLTGHSNSIWAAAYSPDGIHLATGSSDKTLILWNVRQASQLGVPFKKLE
                     GDAWALAVSPDKKHFAAGDTFGGIHVWDFDGNTLSNEQKIPNEGGVLALAYSNDSKWL
                     ASSGYSDNTIRVWDAATGKELWSLENAHDQEIWALAFSPDNRWLASASFDQTVKIWDT
                     TTHELITEPLQHDSGVYTLAFNPNGSSLYVAGFPFNIFSYSLTDTSNIPEPQQLTGHG
                     SAVNLLASNPNYPEFIASTSDDKTLLVWNVANGEHTPPVLGLQESMEAVAFRPSGDWL
                     ASATNNNTVLLWQFDAKRCAKEWDQDTCQPQIIGSPLTGSQTAVYNVIFLSDTVMISS
                     SQGGQLIKWNLDKAYWYDVACSIVNRPLSEAEHRQYVGKQLNSKLLNAITWVSERFGN
                     DVPPAPACLNIDGNN"
     gene            complement(2856..3056)
                     /gene="rpmF"
                     /locus_tag="PLT08_01745"
     CDS             complement(2856..3056)
                     /gene="rpmF"
                     /locus_tag="PLT08_01745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HNE03212.1"
                     /translation="MTPHPKRKHSKGRRDRRRSQDALSAANLVTCSNCGNKRLPHVVC
                     PNCGYFKGREVVQVKKEKKSAE"
     gene            3185..3892
                     /locus_tag="PLT08_01750"
     CDS             3185..3892
                     /locus_tag="PLT08_01750"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HNE03213.1"
                     /translation="MEIQIAVAKINKYAVSESGDTLEVIERPTGGLSVVLADGQTSGR
                     GAKAVSMMVVRKVISLIAEGVRDGAAARAASDALFTERAGKVTCTLNIASVDLHSKTI
                     VLTRNNPAPMYVCRGEEIEIHNEESISLGTSRNLRPIITELNIEAGLTIIIFSDGISH
                     AGERRGTPLDVRHTLLSMLEDQDPSPQQLADTLLAEAVRLDDKRPADDISVLVLRVSP
                     RTGDDVRRMAVRLPINA"
     gene            3885..4946
                     /locus_tag="PLT08_01755"
     CDS             3885..4946
                     /locus_tag="PLT08_01755"
                     /inference="COORDINATES: protein motif:HMM:NF014325.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-binding protein"
                     /protein_id="HNE03214.1"
                     /translation="MHKKFHFIDKHRWRHIQAGIRDTTILLREFRTPLFWFTFAVVGG
                     GILYHYLSQRLNEPVYSLTESIYIVLIATFLQPANREFPHHILLQIFHFVMPVIGLSV
                     LAQGLADFGSLFFNRKSRNKEWEMAVASTMQDHIILVGLGHLGYRVALKLHEMGEPLV
                     VIEFNADADTVNAIKNMGIPVIHDDGTRPAVLEAANIKKSRTIIMASQNDSMNLQIAL
                     KARSLNPNIQVVIRIFDDDFAHALQEQFSFIALSATEMAAPVFASAAAGVDVTNPISV
                     EGQLLSLARLTISKNAPFADKTIGYIEDTYHLNIVLHRKNGHSEMHPTDKTAIHPGDV
                     IAILGGPEQLNKIFHDNGQ"
     gene            4936..5301
                     /locus_tag="PLT08_01760"
     CDS             4936..5301
                     /locus_tag="PLT08_01760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE03215.1"
                     /translation="MDSDPSSSPLIGVVGPCGSGKSTLIRGLEAHGYRCRHIAQEHSY
                     AKQMWQIITKPDILIFLECSFENSTQRRKLNWAAADHEEQLRRLSHAYEHAHIIINTN
                     TLDENGVLAQALDYLKKHL"
     gene            5466..6833
                     /gene="secD"
                     /locus_tag="PLT08_01765"
     CDS             5466..6833
                     /gene="secD"
                     /locus_tag="PLT08_01765"
                     /inference="COORDINATES: protein motif:HMM:TIGR01129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HNE03216.1"
                     /translation="MRNKNTWLIVILFLLAFSLWVNFSKTITVINPFNDNTPVVERDV
                     DIKLGLDLRGGLQALLEADVPADSTVSSADMENAKNILQNRANALGVSEIVMQTAGDR
                     RIVAEFPGVTNPEEVVSALKQTALLEFVDMGTTPVAEGTTIQTDFAAGDTTAAATPAP
                     DGTTPVIYHTVMTGTSLDTASVVRSQTGEYEISFTLKADAASVFGEYTTTHTNQYLAI
                     VLDKVVISTPVIRQPITSGQGVISGNFTSESAQTLAIQLRSGALPIPIKVVESRTVGP
                     TLGEESVRKSVLAGLIGLTVVVLFMGFYYRLPGVIADLALICYAIFSLMLFKLIPVVL
                     TLPGIAGFILSVGMAVDANVLIFERLKEELRAGRNLRHAIDLAWSRAWSSIRDSNTST
                     LITCVILFIFGSTFGASMVKGFAVTLAIGVLVSLFTAITVTRTFLHAILDNVKNTEHP
                     SWFGL"
     gene            6843..>7598
                     /gene="secF"
                     /locus_tag="PLT08_01770"
     CDS             6843..>7598
                     /gene="secF"
                     /locus_tag="PLT08_01770"
                     /inference="COORDINATES: protein motif:HMM:TIGR00966.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecF"
                     /protein_id="HNE03217.1"
                     /translation="MNIIKNRYLYFLISLLVIIPGLVFMGLNWANTKQGPLVLGIDFR
                     GGSLLEIQFEGTRPSSSDIAALYAEFSTEEENFKDPVIQPLGEDAYSIRSKTMTDETK
                     GKLVAEMESRFGSKITVLNFTSVSASVGAEVTRAAGVAIFMAAAAILIYIWWAFRSVE
                     HAYRYGTAAIIAMLHDVFVVLGAEAILGYFFGWEADALFLTALLTVIGFSVHDTIVVF
                     DRVRENSNLGRKIGYESMINHSIVQTLDRSLTTQ"
BASE COUNT         1984 a         1967 c         1717 g         1930 t
ORIGIN      
        1 tgccgcaaag agttagaaat tgcgattgaa tacaacaagc ggctcttccc catcattcac
       61 caaaatattg acccgcaaag ttctcctgcc gcattggcat ccttaaattg gattttcttc
      121 cgcgaagccg atgacttcaa gggtgccatg caaaaattaa tggcagcgat caatactgat
      181 ctggattggg tcaaacaaca tactgttctt ttgaagcgag ccaacgaatg gaattcaaaa
      241 aaacaagatg gcagttatct gttacgcggt agtgaattac aaggggcaga gttatggctt
      301 tctaaaagtt ctgacaataa acaaccacat ccaacgccac ttcaaagtga atacatcttt
      361 gccagccgcc aagatgcaag ccgccgccaa cgcaatacct tgattggcgt cagcgcagca
      421 ttggtcgttt ctattttatt ggcaattgcc gccatcgtcg gcggagtgga ggcggtacgt
      481 aaaagtcagc aagcactggc gagtcagctt gccgctcaat ccatcacttt ggtagacaca
      541 caacctgacc tttctttact tctgagcctt gagtcaaatt acattggcga cactcttggc
      601 gatgcagacc cggcaagttt aggaagtttg gtttccagca tcaacagcgt cccacagttg
      661 gatacttatt tgcgggcaca tgcaggtgag attcgtgaag tgacgttcag ttcgaatgga
      721 agatgggttg cggcaaccgg gaatgcgatc aacgaccaag gcgttgtcac agtttgggat
      781 ctggcttcaa aagaaaaccc gatcgcagat caacaattta ttggcggcac acaacgtatt
      841 ttaggtcttg ctttcagccc agacagcaaa cgcattgcag cggctggaga tgagaaaaaa
      901 atctttatct gggatccaca aacttgctgt gaaaccgtaa aagaatttga aattgacaac
      961 aaagcccgtt ctttaaagtt tgttacccta aacggaaaag aatacatcgc cattgcttct
     1021 ggtcaacaaa tcacattctg ggactcatcc acaggtaaaa aagatgaagc ccttactcta
     1081 caaatctcta ctaccgatga aaccgttcgt attctcagcc ttgccgttgc tccaacaaac
     1141 gatatggtag cagctggaag tgaagacgga aatatcacag tttgggagct aaaaacaaaa
     1201 gaagttaaat tccaagcctg tagtttcggc gaccctgaaa ccaacaacga ggaaatttgt
     1261 aaagtaaacg gagacggtga aacggatatc cgcgggttgg cgttcaatgc aaatggaacc
     1321 ttacttgcct caggaagctc cgaccatcgt gtctggctct ggaatccaga aaccggtgac
     1381 cttttagctc gctctgcggt cagcaccgaa ggtggtcaca tcaacacggt tacctcagta
     1441 accttcaact cgaaaacagg aaccattgct tctgtaagtt gggacaatac cgttcgtatt
     1501 tgggaaccaa ttcagaatga agataaaact tgggggcttg aacttgtaga atctctaaca
     1561 ggtcattcca attcgatttg ggcagcagca tacagcccag atggcattca tcttgccacc
     1621 ggaagttcag ataaaaccct aatcctttgg aatgtaagac aggcaagtca actcggggtt
     1681 ccattcaaaa aattggaagg ggatgcctgg gcattggcag tctcgccaga taaaaaacat
     1741 ttcgctgctg gagatacatt cggcggcatc catgtatggg attttgatgg aaacacctta
     1801 agcaatgagc aaaaaatccc aaacgaaggt ggcgtattag cactagctta cagtaacgat
     1861 agcaaatggc ttgcatcgtc aggctactca gacaacacca tccgcgtttg ggatgccgca
     1921 acaggcaaag aattatggag ccttgaaaat gcccatgatc aggaaatttg ggcgctcgcc
     1981 ttcagcccag ataatcgttg gcttgcctct gccagttttg atcaaaccgt aaagatatgg
     2041 gatacaacga ctcatgaatt gatcacagaa ccattgcaac acgactctgg agtatatacc
     2101 cttgctttca atccaaacgg ttcttccttg tatgtggctg gtttcccctt caatatcttt
     2161 tcctacagcc taaccgatac ttccaacatt ccagagcctc aacaactcac tggtcatggc
     2221 tctgccgtaa atttacttgc ctctaatcct aattatcctg agttcatcgc ctccactagc
     2281 gatgacaaaa cgttgttagt ttggaatgta gcgaatggtg aacacactcc acccgtatta
     2341 ggtttgcaag aaagcatgga agctgtcgcc ttccgtccaa gcggagattg gctcgccagc
     2401 gcaacgaaca ataacaccgt cttgctctgg caatttgatg caaaacgctg tgccaaagaa
     2461 tgggaccaag acacctgcca gcctcaaatt attggctcgc cgctcacggg aagtcagacc
     2521 gccgtctata acgtcatctt tttatcagat acagtcatga tctcatccag ccaaggcgga
     2581 cagttgatca aatggaactt agacaaagcc tattggtatg atgttgcctg tagcattgta
     2641 aatcgcccct taagcgaagc tgaacatcgc caatatgttg gtaagcaact aaattcaaaa
     2701 ttattgaatg caatcacctg ggtttctgaa cgttttggaa atgatgtccc cccagcccct
     2761 gcatgtttga atattgatgg aaataactaa ctgaccgaat aaaaagatgc tgaattaaac
     2821 acaaacgggt cgtgaggttc acgacccgtt gcaatttatt cagccgattt cttttctttt
     2881 ttaacctgaa ccacttcgcg tcctttgaaa tatccgcagt tcgggcaaac gacgtgcggc
     2941 aggcgcttgt tcccgcagtt ggaacaggta acgaggttgg cggcgctcaa ggcatcctga
     3001 gagcggcggc gatcccggcg tccctttgaa tgtttgcgct ttggatgtgg ggtcatgaaa
     3061 cgttctcctt tatacataca tcagacgggc gcacgcccgt ctggattggt tttattacga
     3121 agcccgctga ttatatatgg gggaggcagt gacgtcaagg cgaattcgat gtaaaatcct
     3181 ctatatggaa atccagatcg ccgtagcaaa aatcaacaaa tatgcggttt ccgaaagcgg
     3241 ggacacgctc gaagtcatcg aacgccccac cggcgggtta tctgtcgtct tggcagatgg
     3301 tcaaaccagc gggcgtggcg ccaaagccgt ctccatgatg gtcgtccgca aagtgatcag
     3361 cctcattgct gaaggtgtcc gcgacggcgc cgccgcccgc gccgcctccg atgcgctatt
     3421 taccgaacga gccggtaaag tgacctgcac tctgaatatc gcctctgtag atttacacag
     3481 taaaaccatt gttctcaccc gcaacaaccc cgctcccatg tacgtatgta gaggtgagga
     3541 aattgaaata cacaacgaag aaagcatttc actgggtaca tcgcgcaacc tccgccccat
     3601 cattaccgaa ctaaacatcg aagctgggct gaccatcatc atcttttcag atggtattag
     3661 ccatgccggc gagaggcgcg gcaccccttt ggatgtacgt catacccttc tctccatgct
     3721 agaagaccaa gatccttccc cacaacaact ggcagatact ctgctagctg aagccgtccg
     3781 tctggacgat aaacgcccag cagatgacat cagtgtactt gttctgcgtg tttctccacg
     3841 aacaggagac gacgtccgcc gcatggcagt gcgcctgccc atcaatgcat aaaaaatttc
     3901 atttcatcga taaacatcgt tggcgtcaca ttcaggctgg gatacgtgat accaccatcc
     3961 ttttacgtga attccgcacg cctctttttt ggttcacctt cgccgtagtc ggcggcggaa
     4021 tactctatca ctatttatca cagcgtttga acgaacctgt gtacagcctc accgagtcca
     4081 tctacatcgt tctcatcgct acgtttttac agccagccaa ccgcgagttt ccacatcaca
     4141 tccttcttca aatttttcac ttcgtgatgc cggtcattgg cttaagcgtg cttgcacaag
     4201 gattggcaga tttcggaagt ttgttcttca atagaaaatc acgcaataag gaatgggaaa
     4261 tggcagtcgc atccactatg caagaccaca tcatcctcgt tggcttaggg caccttggtt
     4321 atcgcgtcgc tctaaagcta cacgaaatgg gtgaaccttt ggtcgtcatc gaattcaatg
     4381 cagacgctga tacagtcaac gcaattaaga atatgggcat ccctgttatt catgatgatg
     4441 gcacccgtcc agccgtgttg gaagctgcga atatcaaaaa gtcgcgcacc attattatgg
     4501 caagccaaaa tgactcgatg aacttacaga tcgcgctcaa agctcgctcg ctcaacccca
     4561 acattcaggt agtcatccgt atctttgacg atgactttgc tcacgcgctt caagaacaat
     4621 tctcatttat tgctcttagc gctaccgaaa tggcagcgcc tgtctttgca tctgccgccg
     4681 ctggcgtaga cgtaaccaac ccaatttcag tcgaaggtca actgctaagc ctggcacgcc
     4741 taaccatttc aaagaatgct ccttttgcag ataaaaccat cggctatatt gaagatactt
     4801 accatctcaa tattgtgttg catcgcaaaa atggtcattc agagatgcac cccaccgaca
     4861 aaactgccat ccaccccggc gatgtgattg ccatcctcgg cggacctgaa caattaaaca
     4921 agatattcca cgataatgga cagtgaccct agtagttccc cactaatcgg tgttgtgggt
     4981 ccgtgcggtt cgggcaaatc cacattaatt cgcgggttag aggcgcatgg ctatcgttgc
     5041 cggcatattg cgcaggaaca ttcctatgcc aaacaaatgt ggcagatcat caccaaacct
     5101 gatatcctca tttttctaga atgttctttc gaaaactcca cccagcgccg caaactcaac
     5161 tgggcagccg ccgaccacga agaacaactc cgccgccttt cccacgcgta cgaacacgct
     5221 catatcatta ttaacacgaa cacgctggac gaaaacggag tccttgcgca agccctagac
     5281 tacctaaaga agcacctcta gccccaagcc ttgccccgcc tcaggcgtgg accctgtttg
     5341 ggtccacaga ccaagccttt cccacgctgg aggcgggtcc ttcttcctcc aaaaaatcaa
     5401 tcaaccagag tataatgtcg tccgcgccag ccaaaggctg gcattttcgt ggatggagta
     5461 aacacatgcg taataagaat acatggctga ttgtgatcct ctttctgctg gcattctccc
     5521 tttgggttaa tttcagcaaa accatcacag ttatcaatcc ctttaacgac aacacccctg
     5581 tagttgaaag agatgttgac attaaacttg gtctcgactt gcgtggcggc ttgcaagcgc
     5641 ttctcgaagc cgatgtccct gccgattcaa cggttagcag cgcagatatg gaaaacgcca
     5701 agaacattct gcaaaaccgc gcgaacgccc ttggcgtgag cgaaattgtg atgcagaccg
     5761 ccggcgaccg ccgtattgtg gctgaatttc cgggtgtaac caacccagaa gaagtggtta
     5821 gcgctttgaa gcaaacggct ttgctagagt ttgttgatat gggcacaacc ccggtagctg
     5881 aaggtaccac cattcaaacc gacttcgcgg caggcgatac aacggctgct gctacccccg
     5941 cccccgatgg cacgacacct gtgatctatc acaccgtgat gaccggtacc agcttggata
     6001 cagcaagtgt tgttcgcagc cagaccggtg aatatgaaat ttcattcacg ttgaaggctg
     6061 atgccgctag tgtctttggt gaatacacca caacccatac aaatcaatat cttgcaatcg
     6121 tgctggataa ggttgtgatt tctacccctg taattcgcca acccatcaca agcggacagg
     6181 gcgttatctc tgggaacttc acttccgaat ctgcccagac cctcgccatt cagcttcgtt
     6241 ctggtgcctt gcccattccg atcaaggttg ttgaaagccg cacagttggt ccgaccctcg
     6301 gtgaggaaag tgtgcgtaag agcgtgttgg caggtctcat tgggttgacc gttgtggttc
     6361 tcttcatggg cttttactat cgcctccccg gcgtcattgc agaccttgcg ttgatttgtt
     6421 acgccatttt ctctttgatg cttttcaagt tgatccctgt ggttttgacc ttgcccggta
     6481 tcgctggctt tattctttct gtgggtatgg cagtagatgc caacgtgttg atctttgaac
     6541 gcttgaagga agaattacgc gctggacgca atctgcgtca cgccatcgat cttgcttgga
     6601 gccgtgcttg gtcttccatt cgagactcca acacatcgac actcatcaca tgtgtcatct
     6661 tgttcatttt cggcagcacc tttggcgcaa gcatggtaaa aggttttgca gttacattgg
     6721 caattggcgt gctcgtcagc cttttcactg ccatcaccgt aacacgcacc ttcctgcatg
     6781 ccattctaga caatgttaag aataccgaac accccagctg gtttgggctt taggacaata
     6841 ccatgaacat aatcaagaat cgctatctat actttctgat ctccctgctt gtgattatcc
     6901 cagggttggt cttcatggga ctaaattggg caaacacgaa acagggtccg ttagttcttg
     6961 gaattgattt ccgaggcggc tccttattag agatccagtt tgaaggcact cgcccctcat
     7021 cttcagacat tgctgctctt tacgccgaat tctctacaga agaagaaaac ttcaaagacc
     7081 ccgttattca accgctcggt gaagatgcct attccattcg ctcaaagacc atgaccgatg
     7141 aaaccaaagg caagcttgtt gctgaaatgg aatctcgttt tgggtccaaa atcactgtgt
     7201 tgaacttcac ctctgttagc gcctctgttg gtgctgaggt gactcgtgct gcaggtgttg
     7261 ctattttcat ggcagcggct gcgatcctca tctatatttg gtgggcgttc cgaagtgtgg
     7321 agcacgccta tcgctacggc acagcggcaa tcatcgccat gttgcatgat gtattcgttg
     7381 tgcttggtgc cgaagccatt ttgggctatt tcttcggctg ggaagcagat gcgctcttcc
     7441 tcactgcgct cctcaccgta atcggttttt cagttcacga cacaattgtg gtgttcgaca
     7501 gagttcgcga aaattccaat cttggtcgca aaattggtta tgaatccatg atcaatcact
     7561 ccattgtcca aaccttggac cgctctctga ccacccag
//
LOCUS       DAOQJE010000037         7540 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_5352_length_7540_cov_6.015631, whole genome shotgun sequence.
ACCESSION   DAOQJE010000037 DAOQJE010000000
VERSION     DAOQJE010000037.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 7540)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7540)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7540
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5352_length_7540_cov_6.015631"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1415
                     /locus_tag="PLT08_01775"
     CDS             <1..1415
                     /locus_tag="PLT08_01775"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HNE03218.1"
                     /translation="FFSMGLLVAMEGGRSTDARLRMALRPLAGFGLVHAAHEWLEMFR
                     AMGHLNAFFTGIYPLLALSILAFSFLSLAAFGAYLVLGSESTWRVSLIIPLGLEAIWV
                     YGLLIFKSKYSLDEIYTVADVWTRYSIAIPAGLLAAAGLVVQQRAFRRAGLVSFGRDS
                     LWASVAFIWYSLVGQLFTEPSPLPPSTFLNSALFLNLFGFSIQLLRAITAMFASLFVT
                     RFLRAFQVESDSKIAGLQAAQLHESQQREKLRAQLFRSIVGAQESERQRIARDLHDET
                     GQSLTAIGMGLRGLANETDSKKRLVTLTQLQSLTSDSIRELQRIISDLRPAHLDDLGL
                     SATLRWYASRVHELTSLHVRVDIEGDEPALDDAVKITIFRIMQEALNNVIKHAQATTV
                     NVKIHYLQKEVRIHVFDNGVGFEMKAVQNRIGRVSLGLAGMEERAALLGGGVTVSSRP
                     GYGTEVEAVVPLHLIDQEEK"
     gene            1412..2062
                     /locus_tag="PLT08_01780"
     CDS             1412..2062
                     /locus_tag="PLT08_01780"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HNE03219.1"
                     /translation="MTIRLLLVDDHAVVRTGLKMLLNGQEDLEIVGEAGSAAEALTET
                     ERVQPTVILMDIGLPDKTGIEATREIKAKFANVNIVALTIHEDEEYFFQMLNAGASGY
                     VPKRAAPEELITAIRAAANGEVYLYPSMAKLLVRDYLNIDRLPEEKIDLGGLTDREHE
                     VFTMLAEGASNQEIADALVISPKTVERHRENIMRKLNLHSRSELVRYAIRKGIIKA"
     assembly_gap    2253..2352
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            2622..2966
                     /locus_tag="PLT08_01785"
     CDS             2622..2966
                     /locus_tag="PLT08_01785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE03220.1"
                     /translation="MTTNSILVFILGVAVRLGLPMLVTTLVVIGLRRLDVRWQKEAEI
                     ERNLLVRDETPCWKEQGLSMDEIKARATESGKPCWQIHRLSNGYMREACLGCEIFINA
                     PTPVLKPSKAHA"
     gene            3001..3993
                     /locus_tag="PLT08_01790"
     CDS             3001..3993
                     /locus_tag="PLT08_01790"
                     /inference="COORDINATES: protein motif:HMM:NF024827.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c3 family protein"
                     /protein_id="HNE03221.1"
                     /translation="MFSRFEKIILALMVSLLIAGVSILAASAQDANPPTPTGDEEETC
                     ADCHEEFEMSWKNGPHGNATSDPIFVKDWTDQGKPSACLACHVTGYDENTGAWEKDGV
                     YCTACHLDDGGDHPKTTMSVDATGGTCGTCHTDARFGMKEWEESTHFAAGMDCTTCHD
                     PHNASLKITVNLKEQKVADASQLCISCHEEASMNFPYSTHSKQGVTCIDCHLEHVESD
                     AAVHQVADHSFKASITTCNSCHANQMHTDGQAEATEQAIVASVAPTETPKPAAATVDQ
                     SPIVSPDPAPVSPLGYAGITALIGIAAGMLLAPWLERGYRLVLKKSAEVHHGSK"
     gene            3980..4756
                     /locus_tag="PLT08_01795"
     CDS             3980..4756
                     /locus_tag="PLT08_01795"
                     /inference="COORDINATES: protein motif:HMM:NF024634.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HNE03222.1"
                     /translation="MAQNKFNRRDFIKLSAVGIAAAAGTRLLTEEAKASEGVKGEHQW
                     VMVIDQSKCTGCNQCNMACHATNDINPEMEWTKVLDMGEMNGKQVYLPRPCMQCEHAP
                     CVEVCPVGASYYRDDGIVMMDYDKCIGCRYCMVACPYDARSFNWEKFDGENPYVPQYG
                     EPEVERRPRGVPEKCAFCYQRIDRGLSLGLKPGEDPAATPACVVACPTGARIFGDLND
                     PNSNVSQLLKDHPSYRLRENLGTKPRVYYLPVNDDTTVEG"
     gene            4758..6023
                     /gene="nrfD"
                     /locus_tag="PLT08_01800"
     CDS             4758..6023
                     /gene="nrfD"
                     /locus_tag="PLT08_01800"
                     /inference="COORDINATES: protein motif:HMM:NF015851.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysulfide reductase NrfD"
                     /protein_id="HNE03223.1"
                     /translation="MKTILKTIRENIFTPWGLWLAFLGLLLVSALVAALTVFWKGLVI
                     TNLTNLVPWGLWITIDLSSIAVAAGAFSLCAIVYLFGFKRYEPIARTATFIGFTGYSM
                     AMMALLLDIGRPERFWKSFVFWNPHSLLWEVTMCVGLYFTVLIFEVLPILASFEWLRT
                     KYPKIAEKMEHMHHYAPVLAVLGLALSSLHQSSLGAVYGVIKARPIWYRPEMSVMFMF
                     TAILGGVSLTLLASMLAARITPKAKVNDTLIERVAQVLGYMMIAYLYWRFWDWLAQTY
                     TYEPGRTEGFRLLTKGPLSFNFWFGEMLFGALFPTIILLKRSLRQTPLWRMLAFAMIV
                     GGVVAFRFDTNIVGFLAVIPYTPGEMIVQYTSYTPSLVEWAAGLGIIAFGLLAFSVGV
                     KYLRVVDHRLVSEEYEVITMKVGEAVTGD"
     gene            complement(6085..6939)
                     /locus_tag="PLT08_01805"
     CDS             complement(6085..6939)
                     /locus_tag="PLT08_01805"
                     /inference="COORDINATES: protein motif:HMM:NF013460.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b/b6 domain-containing protein"
                     /protein_id="HNE03224.1"
                     /translation="MNTVTKAYLRFPIARRIEHWVMMLSFTLLGLTGLPQRFPEAGIS
                     QAILGVLGGIQGVRTIHHTSAIVMMFGTAWHILVMGYSVFVLRDKMSMLPTLQDAKDG
                     LQALLYNIGFAKTYPQMGRYTFEEKMEYWAFVWGALVMGATGFMMWNPITATKFLPGE
                     FVPAAKAAHGNEAVLAVLAIIIWHMYGVHIKRLNKAMFNGMQTEAEMLHEHPLELADI
                     KAGIADRRPDAATLRKRQMIYYPIAAVLTIGMLSGIYGFINAEETALTTIPPIVETVE
                     PPAPTVQP"
     gene            complement(6939..>7540)
                     /locus_tag="PLT08_01810"
     CDS             complement(6939..>7540)
                     /locus_tag="PLT08_01810"
                     /inference="COORDINATES: protein motif:HMM:NF021235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="cytochrome c3 family protein"
                     /protein_id="HNE03225.1"
                     /translation="CHSETVELYKGSVHGSALADGNTDVPTCTDCHGVHNIGDPTTAS
                     FHNNTPALCADCHDNEELMGKYGLSTEVLETYVADFHGTTTTLFEQQFPGQPINTAVC
                     TDCHGVHDIASTKNPETGIAMQENLLVKCQRCHPDATANFSAAWMGHYQPSPEHYPIV
                     YYVNLFYKFFIPAVLGGMVLFILTDIYRRIINRAKGAKH"
BASE COUNT         1758 a         1876 c         1954 g         1852 t
ORIGIN      
        1 cctttttcag catgggcttg ttggttgcca tggaaggagg gcgctctaca gatgcgcgct
       61 tgcgtatggc gttacgtccg ctggctggct ttggtcttgt acatgccgcc catgaatggc
      121 tagaaatgtt cagagccatg gggcatttga atgcattttt tacaggcatt tatccactgc
      181 tggcattatc cattttggct ttttcgtttt tatcactagc agcattcggc gcttatctcg
      241 tccttggcag cgaatcgact tggcgtgtga gtctcatcat tccactgggg ctcgaagcca
      301 tctgggttta tggcttgctt attttcaaaa gtaaatattc gctagacgag atttacaccg
      361 tcgccgatgt gtggacgcga tactccattg ccattccagc agggttgttg gcagcggctg
      421 ggttggtggt gcaacaacgt gcgttccgca gagccggttt ggtaagcttc gggcgcgata
      481 gtttatgggc atcggtggcg ttcatttggt atagcttagt aggtcaatta tttacagagc
      541 caagcccatt accaccttca actttcttaa actcggcact gttcttaaat ctatttgggt
      601 tttccattca gttactacgc gcaattactg ccatgttcgc ttccttgttc gtaacgcgtt
      661 ttttgcgcgc ctttcaagtg gaaagcgaca gcaagatcgc aggactacaa gccgcacaat
      721 tacacgagtc acaacaacgc gaaaaactgc gcgctcaact gtttcgcagc atcgtgggtg
      781 cccaagagtc tgaacgtcaa cgcattgcac gcgatctgca cgatgaaacc ggtcagtcct
      841 taactgccat tggcatgggg ttgcgcggtc ttgcgaatga aactgactca aaaaagcgcc
      901 tcgtcactct gacccaactc caatctctta cctcagactc catccgtgaa ttgcaacgca
      961 tcatttcgga cctcaggcct gcgcacctcg atgacctggg tctttctgcc acgttgagat
     1021 ggtacgcttc gcgcgtgcac gagctgacat ccctgcatgt acgcgtggac atcgaaggcg
     1081 atgagcccgc tcttgacgac gccgtgaaaa tcaccatttt ccgcattatg caagaagcgt
     1141 tgaataacgt catcaaacat gctcaagcga caacggtaaa cgtcaagatc cattatttgc
     1201 aaaaggaagt tcgcattcat gtgtttgata atggcgtggg gtttgaaatg aaagccgtac
     1261 agaaccgcat tggacgtgtc tctttaggct tggcaggcat ggaagaacgt gctgcgttac
     1321 tcggtggcgg tgtgaccgtc tcttcacgcc caggctatgg gacggaagtt gaggcggttg
     1381 ttccgttaca tttgatagat caggaggaga aatgacgatc cgattgttat tggtggatga
     1441 ccatgccgtt gtccgcacag ggttgaagat gttgctcaac gggcaggaag accttgagat
     1501 tgttggggaa gcggggtctg cggcagaggc attaaccgaa accgagcgcg ttcaaccgac
     1561 ggtcattctg atggatattg gtttgccaga taaaaccggc atcgaagcca ctcgtgagat
     1621 caaggcaaag tttgcgaatg tgaatatcgt tgcgctcacc attcacgagg atgaagaata
     1681 tttcttccaa atgttgaatg ctggtgcctc tggctatgta cccaaacgcg ccgcaccaga
     1741 agagctcatc accgccattc gggcagcagc gaatggggag gtgtatcttt acccgtctat
     1801 ggcgaagctc ttggtgcgcg actatttgaa cattgaccgt ttgccagaag agaaaattga
     1861 cctaggcggg ctgaccgacc gcgagcatga agtctttacg atgttggcag aaggggcgag
     1921 caatcaagaa attgccgatg cgcttgtgat cagcccaaag acggtggagc ggcatcgcga
     1981 gaatatcatg cgcaaactaa atctccactc ccgttcagag ttagtgagat atgccattcg
     2041 gaagggaatc atcaaggcat agataacaaa atccataaat cacgcatccc gtaatatggg
     2101 atgcgttttt atttaagttc gcgtggtggg tgtcaagcga gtaagtttat ccactttaca
     2161 cacatgtttt tttcgatggg gcagataccc cacagcttga ataaggcttg gggtgttcgc
     2221 cacaggcaaa gggtcccaaa agatgcggag ggnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2281 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2341 nnnnnnnnnn nntgaataag gcttggggtg ttcgccacag gcaaagggtc ccaaaagatg
     2401 cggagggtac tcatccaaga atggggttgg tgacgaattt actttccttt aaggaagcaa
     2461 tatcctatag gcagatttga gaaaggagaa tgtcatgaac ggttctgagt ttttcatcct
     2521 gtactttatc gcccgcgttg ccatcccatt tggagtgctt ctgctgctgg gcgagttgtt
     2581 acgccgcaaa gaatctaact actggctcaa gtgaggtcga catgactacg aattcaattc
     2641 tcgtttttat cctgggtgtg gctgtgcgat tgggtttacc catgttggta accaccttgg
     2701 tcgtgatcgg tttacgccgt ctggatgtgc gctggcagaa ggaagcggag atcgaacgca
     2761 accttctggt acgcgatgag acaccctgct ggaaggaaca aggactttcg atggacgaga
     2821 tcaaagctcg cgccactgaa agcggaaaac cctgctggca gatccaccgt ctttcaaatg
     2881 gctatatgcg tgaagcctgc ctcggttgcg aaatctttat caatgcgcct acaccggtgc
     2941 taaagccatc gaaagctcac gcttaactct taagtttcac tcttaatagg agatgctctc
     3001 atgttctcac gttttgaaaa aatcatcctc gcccttatgg tttccctgct gattgcaggt
     3061 gtttccattc tcgctgcttc cgcacaagat gccaatcccc ctacgcccac aggggatgaa
     3121 gaagaaacct gcgcagactg ccatgaagaa tttgaaatga gctggaagaa cggtccgcat
     3181 ggaaatgcca ccagcgaccc gatcttcgtc aaagattgga ccgaccaagg caaaccaagt
     3241 gcttgtctcg cttgccatgt gaccgggtac gacgaaaaca ctggcgcttg ggaaaaagat
     3301 ggcgtgtatt gcacagcttg tcatttagac gatggtggcg accaccccaa gaccaccatg
     3361 agtgtggatg cgaccggcgg tacctgcggt acttgtcaca ccgatgctcg ttttggtatg
     3421 aaagaatggg aagaaagcac tcacttcgct gccggcatgg actgcacaac ctgccatgac
     3481 ccacacaatg cttctttgaa gatcaccgtc aacctcaaag aacaaaaggt ggcagatgct
     3541 tctcagttgt gcattagctg ccacgaagaa gccagcatga acttcccata ctccacccac
     3601 agcaaacaag gcgtcacttg tatcgattgt catcttgaac atgtggaaag tgatgcagcg
     3661 gttcaccaag tggcagacca ctcgttcaaa gcaagcatta caacctgtaa ctcttgccat
     3721 gccaaccaaa tgcatacaga tggtcaggct gaagcgacgg aacaagctat tgttgcgagc
     3781 gttgccccaa cagaaacacc caaacctgcc gccgctacgg tagatcaatc tcctatcgtt
     3841 tcaccagacc ccgctcctgt ttctccgctc gggtatgcag gcatcacagc actgatcggc
     3901 attgccgctg gtatgttgct ggctccctgg ctcgaacgcg gataccgcct cgtattgaag
     3961 aaatctgctg aggtacacca tggctcaaaa taagttcaac cgccgtgact ttatcaagct
     4021 ttctgcagta ggcattgccg ctgctgcggg aacgcgtctg ctcactgaag aagcaaaagc
     4081 ctcagaaggt gtaaaaggcg agcatcaatg ggtaatggtc attgaccaat ccaagtgcac
     4141 gggctgtaat caatgcaata tggcgtgcca cgccaccaac gacatcaacc ctgagatgga
     4201 atggaccaag gtgttggata tgggcgaaat gaacggcaag caagtctatc tgccgcgccc
     4261 atgtatgcaa tgcgaacacg ctccctgtgt ggaagtctgc ccggtgggtg ccagctatta
     4321 ccgtgatgac ggcattgtga tgatggacta cgacaaatgc atcggttgcc gctattgcat
     4381 ggtggcatgc ccatacgatg cacgttcctt caattgggaa aagttcgatg gcgagaatcc
     4441 ctacgtgccg caatatggcg aaccagaagt ggaacgccgt ccgcgcggtg tgccagagaa
     4501 atgtgcgttc tgctatcaac gcatcgaccg cggactttca ctggggctta aacccggtga
     4561 agaccccgcc gccaccccag cttgtgtagt tgcctgccca accggcgccc gcatctttgg
     4621 tgacttgaac gacccgaatt ccaatgtgag ccaactcttg aaggatcatc cgtcctatcg
     4681 cttgcgcgaa aatcttggaa ccaaaccgcg ggtctattac ctgcccgtaa atgacgacac
     4741 cactgtggag ggatgacatg aaaaccatcc taaaaaccat tcgagaaaat atctttaccc
     4801 cttgggggtt atggctcgct tttctagggc ttctgttggt cagcgcattg gtcgccgcac
     4861 tcaccgtctt ctggaaaggc ttggtcatca ccaatctcac caacctagtc ccttggggtt
     4921 tgtggatcac catcgatctc tcgtccattg ccgtggcggc tggtgccttc agtttgtgcg
     4981 caattgtgta cctcttcggc ttcaaacgct atgaacctat tgcccgcacg gcgaccttca
     5041 ttggcttcac gggctacagc atggcaatga tggcgctatt gctggatatt ggtcgccctg
     5101 aacggttctg gaaatccttt gtcttctgga atcctcactc tctgctttgg gaagtaacca
     5161 tgtgtgtggg tctatacttc acggtattga tcttcgaagt cttgcccatc ctcgcatcct
     5221 ttgaatggtt acgcaccaaa tacccgaaga tcgccgaaaa gatggaacac atgcatcact
     5281 acgcacctgt gcttgccgtc ttaggcttgg cgctctccag tttgcatcaa tcttcactcg
     5341 gtgctgtata tggcgtcatc aaagctcgcc ccatttggta tcgccctgaa atgtctgtga
     5401 tgttcatgtt cacagccatc ctcggcggcg tatcgctcac actgttggca tccatgttgg
     5461 cagcacgcat tacgcccaaa gccaaggtca acgatacatt gatcgagcgt gtcgctcaag
     5521 tgcttgggta tatgatgatc gcttacctct actggcgctt ctgggattgg cttgcccaaa
     5581 cctacaccta cgaacccgga cgcaccgaag gattcagact cctcaccaaa ggaccgctct
     5641 ccttcaactt ctggttcggc gagatgctct tcggcgcgct ctttcctacc attattctgc
     5701 tcaagcgctc cctgcgtcag actccgctgt ggcggatgct cgccttcgcg atgatcgtcg
     5761 gcggcgtagt agccttccgc ttcgatacga atatcgttgg tttccttgcg gtcattccgt
     5821 atacccccgg tgagatgatc gtccagtaca ctagctacac tccttcgctc gtagaatggg
     5881 cagccgggtt gggaatcatc gcctttggtc tgctggcttt ctccgtcggt gtgaaatact
     5941 tacgcgttgt agatcatcgc cttgtgagtg aagaatatga agttatcacc atgaaggttg
     6001 gcgaagccgt tacgggagat taaggttcgg tatagaatca aaaaagagcc gcctctaagg
     6061 gcggctcttt tttgattctt gttcctatgg ttgaaccgtt ggtgcaggtg gttcgaccgt
     6121 ctccacaatg ggcggaatgg tcgtcagcgc ggtttcttca gcgttgataa agccatagat
     6181 tccactgagc atgccgatgg ttaagacagc ggcgatggga tagtagatca tctggcgctt
     6241 gcgcagagtc gcggcatcgg ggcggcggtc tgcaatccca gctttgatgt ctgccaactc
     6301 aagcggatgt tcatgcagca tttcggcttc ggtttgcata ccgttgaaca tggctttatt
     6361 caggcgtttg atgtgcacgc catacatgtg ccagatgatg atagcaagca cagccaacac
     6421 agcttcgtta ccatgagcag ctttggcagc cggtacaaat tcgccgggca ggaactttgt
     6481 tgctgtaatt ggattccaca tcatgaaacc agtggcgccc atgaccaatg cgccccaaac
     6541 gaacgcccag tattccatct tctcttcgaa ggtgtagcgc cccatttgtg ggtaggtttt
     6601 ggcaaagcca atgttgtaga gcaatgcctg caaaccatct ttggcatctt gtaatgtagg
     6661 cagcatggac atcttatcgc gcaagacgaa gacgctgtac cccattacga gaatatgcca
     6721 ggcggtgcca aacatcatga caatagcaga cgtgtgatga atcgtgcgca caccctgaat
     6781 gccgcccaag acaccgagaa tggcttgtga aattccagcc tccggaaagc gctgaggaag
     6841 cccagtgaga ccgagcaagg tgaaagagag catcatcacc cagtgttcaa tacggcgcgc
     6901 gatcgggaag cgcaggtaag ctttggttac agtattcatt agtgctttgc tcctttcgcg
     6961 cggttgatga tgcggcgata aatatcggtg aggataaaga gcaccatacc acccaacact
     7021 gcgggaatga agaatttata gaagaggttg acgtaataga cgatcgggta atgctcaggg
     7081 ctgggttggt agtgtcccat ccatgcggct gagaaattag cagtggcatc ggggtgacag
     7141 cgctggcatt tgacgagcag attctcctgc atggcaatac ccgtttcagg gttcttggtg
     7201 ctggcgatgt cgtgaacacc atggcagtct gtacagacgg cggtgttgat cggttgaccg
     7261 gggaattgct gttcaaagag tgtggtggtg gtaccgtgga aatctgccac ataggtttca
     7321 aggacttcgg tggaaagacc atatttgccc atcaactctt cgttatcgtg acagtctgcg
     7381 cacaaggcag gtgtgttgtt gtggaaagag gcagtggtgg ggtcaccgat gttgtgcaca
     7441 ccgtgacaat ctgtacaggt gggcacatcc gtattgccat ctgccagagc actgccgtgg
     7501 acactgccct tgtagagctc aacggtttca gagtgacact
//
LOCUS       DAOQJE010000038         7502 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_5393_length_7502_cov_6.241977, whole genome shotgun sequence.
ACCESSION   DAOQJE010000038 DAOQJE010000000
VERSION     DAOQJE010000038.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 7502)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7502)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7502
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5393_length_7502_cov_6.241977"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            427..711
                     /gene="rpsF"
                     /locus_tag="PLT08_01815"
     CDS             427..711
                     /gene="rpsF"
                     /locus_tag="PLT08_01815"
                     /inference="COORDINATES: protein motif:HMM:TIGR00166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HNE03226.1"
                     /translation="MRNYELMCIIQPDLDETAMNGVLDKVKGWITEAGGSIDKAEVWG
                     RRRMAYSINKHREGQYVLLNVSMSPAATSDLERNLRYQESIMRHMLSVVG"
     gene            748..1155
                     /locus_tag="PLT08_01820"
     CDS             748..1155
                     /locus_tag="PLT08_01820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HNE03227.1"
                     /translation="MSRGLNKVQIIGHLGKDPEMRYTPSGKPVTTFTVAVSRTWNSAD
                     GERHNETEWFNVVAWGTLAEICKQYLVKGQQVYVEGRLQTRRWDDKEGIKHTSVEIVA
                     NEMMMLGDRREANNQPDASPAESNGAADEEFPF"
     gene            1206..1499
                     /gene="rpsR"
                     /locus_tag="PLT08_01825"
     CDS             1206..1499
                     /gene="rpsR"
                     /locus_tag="PLT08_01825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HNE03228.1"
                     /translation="MSDERPERPERSGGEGGGERGDRKFFAKPKFCQFCADKTLTIDY
                     KKVDLLRKYVTEEGSLRPRRQTGACAKHQRLVAAAVKQARHVALLPFANRGVD"
     gene            1605..3038
                     /locus_tag="PLT08_01830"
     CDS             1605..3038
                     /locus_tag="PLT08_01830"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF025010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HNE03229.1"
                     /translation="MTNESGKKPAMHKKHVARLERELQQTRLILYTFFGILGVVAVLL
                     IYGWLDVNYLQLNRAVATVNDNKIVLKDFEARTRLRRQQLIDNYNQYTQYQQYFGMDM
                     SSQLNSIETQLNTPELVGTSVLDAMIDEEIIRLEAEKRGITVSEEEIDKKMEEGFNFY
                     PNGTPTPSLTPTPADLPTDPAELLQFITATPDVTATPLFTATAEFTATVESQPTAEGD
                     ATATLAPTNTAEPTVTPTATLGPTSTPEPTATPFTREAYEVLLGKTKDNITKYGFDET
                     YIREFFRNQILREKLLEVIAADVKPFEEQVRARHILVADEQTAKDLIARLQAGEDFAD
                     LARQYSSDSSSEQGGDLGWFGKGQMVAEFETAAFALQNPGDFTTEPVASQFGYHIIQL
                     MAKQERPLSASELQNAKETALQDWLTKARDTDYTVETFDFWKQRVPSTPNFITAATES
                     AAAQKTAQAESVATLDAKNSAAATPTP"
     gene            complement(3101..4156)
                     /locus_tag="PLT08_01835"
     CDS             complement(3101..4156)
                     /locus_tag="PLT08_01835"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HNE03230.1"
                     /translation="MNDLDEKRKYSLLRIFFSLSLVINAFFVVLQLYLKNTELVLVAL
                     INIVVFGGNFYLLQKRKYLLAMHIGITAITIASLVSNFYLGWESYFSLYLIASTIIII
                     NSVTLSLRRKLIEVTALMTLFVASFIHMKTFTPLYDLNPVLVERIGLFNSGAVLFSVL
                     FMQFHNFIENENLKKKLQEMSEIDPLTGAYNRRFFNEYLDIELKRLESPSRYAPAKKT
                     NFGIAIIDIDDFKMINDMYGHLVGDMVIAEVAGIIKSAIFKRDIFCRYGGEEFLVLFT
                     NTSKSGVKIAIEKIRTAIQNHVFMTHGERLNIEVTVSVGFAGFSEEANVYKLLDLADK
                     RLYKVKGAGKNGAILGE"
     gene            complement(4256..4876)
                     /locus_tag="PLT08_01840"
     CDS             complement(4256..4876)
                     /locus_tag="PLT08_01840"
                     /inference="COORDINATES: protein motif:HMM:NF014845.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-vinyl reductase"
                     /protein_id="HNE03231.1"
                     /translation="MINPEEAVIINSLVRQALTSMQEVMGDNGLNAVLKSCGLERFVG
                     NFPPNNLEPAIKTSQYAQLNQAIEDFYGRGGKGMLQRIGRASFQYGVREQAALLGVAG
                     VALKLMPEKQRIKFILNGMADALKKSNAQVKAWVDESGERLAYVDATCGICNSRHSDH
                     PICHLYIGSVSEAVKWATGKDYEVIETHCTAMGDEYCRFEVGNVKE"
     gene            complement(4986..5612)
                     /locus_tag="PLT08_01845"
     CDS             complement(4986..5612)
                     /locus_tag="PLT08_01845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE03232.1"
                     /translation="MMTENFIFPFELQQSRPFHFVEETSLAAARIGGTMPDGISVPLR
                     DDARYFGTFPLYDGNSRLFFSIFINGSFASFIHALNAGFQTDDRVVVIVHEKKPRSTD
                     TKYASGLSPHAIQVHDIASDLIQNDIGETVIRERHKFGGRPYSIQEPILPGSEALFAQ
                     GYRQVLQIDFPNPTDGAISGSWPFGDGIFNLFWKAPFEKEPYFWCLQG"
     gene            complement(5623..6426)
                     /locus_tag="PLT08_01850"
     CDS             complement(5623..6426)
                     /locus_tag="PLT08_01850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE03233.1"
                     /translation="MTILYKQALDLLDARSLVTSLDHDLTRLIRRELSASPGGSDDIA
                     DRVLKNILLRWNCALSAADDEVTARQKNLIVVTLTLLLHKYGIQDPSLNSKAYQAIEA
                     LNREVILSDSFYRKSSAIKELLSSPVALRSRKPARPADLTFWRTGDLVAYQIGGKFYA
                     LYIHGIVGANEAPIVEFMDMETSYRPVRDDVLGKKARGIPFKDGVLHVQHYLVYGMRD
                     MPDPANQFHLLGNMPQYKPDQTHLAPPIGMGIVTDVFRLADEVREKLFP"
     gene            complement(6841..>7502)
                     /locus_tag="PLT08_01855"
     CDS             complement(6841..>7502)
                     /locus_tag="PLT08_01855"
                     /inference="COORDINATES: protein motif:HMM:NF012792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNE03234.1"
                     /translation="DYPALFKEILAWADGRAQAVTGTPYERSAWFVNVFSGQESRKKD
                     LEEMGFACQSDVGEDSWSKVFMKLSPEPKLYEPPAGFTVRPLGGDVEKYVALHQSVFE
                     SKNMTIEWRGRTLQSPLYRPELDLVVESPTGELAAFCICWFNEKLSAGQVEPLGVHAD
                     YRQLGLGRVALSHGVNRLRSLGAKDIFVETDNYRNEAFNLYQSIGFEVIQDVLVYRKD
                     Y"
BASE COUNT         2071 a         2064 c         1603 g         1764 t
ORIGIN      
        1 tctttatttg ttattactgt ttgccaactg ctgcttatac agcatactgt aaagtctcct
       61 gcgggataaa caacacccga aactagggta cataacagcc aaataaaacc ttatacacac
      121 caaattttac acccatattt tcgataatta cagttattct catcactgat cgtctgtatc
      181 aattacccac aaaaataaat tccaccactc gcaaacagct acaaacggca ttatagcacg
      241 caggttgtgg gtgtatccct acgcgtggct taaagttttc tgtcagcccc taatcccgta
      301 aaagcgattc ctgtggtaaa ctttcgccgc gaaattaggt acgttacatt tctatacgtg
      361 cctttccgtt cccggcaaac ccacggtttg aacaggaaca aacccatgga aaggaggttt
      421 tcccacatgc gtaattatga actgatgtgc atcattcagc cagacctgga tgagaccgcc
      481 atgaatggcg tgctcgacaa ggtgaaaggt tggatcactg aagctggcgg tagcatcgac
      541 aaagctgagg tttggggtcg ccgtcgtatg gcgtattcca tcaacaagca ccgcgaaggt
      601 caatacgttc tcttgaacgt atccatgagc cccgccgcca catcggacct tgaacgcaac
      661 ctgcgttatc aagagtccat tatgcgacac atgctttctg tagttggata atccgttttt
      721 gcccggcatt ctacttagga gtgacttatg agccgaggac ttaataaagt acaaatcatt
      781 ggacatttag ggaaagaccc cgaaatgcgc tacaccccca gtggcaaacc cgtcaccacc
      841 ttcacggtgg cggtcagccg cacctggaac agcgcagacg gcgagcgcca caacgaaacc
      901 gaatggttca atgtcgtggc gtggggaacg cttgctgaga tctgcaagca atatctcgta
      961 aaaggtcagc aggtctacgt agaaggacgc ctgcaaaccc gccgctggga tgacaaagaa
     1021 ggcattaaac ataccagcgt agagatcgtt gccaatgaaa tgatgatgct cggcgaccgg
     1081 cgcgaagcga acaaccagcc agatgcctcc cctgctgagt caaatggcgc agcagatgag
     1141 gaatttccgt tttagttctt gccattctga acaaaagctc agagtgacat caattggagt
     1201 aacccatgag tgacgaacga cccgaacgac ctgaacgctc aggcggcgaa ggcggcggcg
     1261 aacgcggtga tcgcaaattc tttgcgaagc ccaagttctg ccagttctgc gccgacaaaa
     1321 ccctgaccat cgactacaaa aaagtcgacc ttcttcgcaa gtatgtaacc gaagaaggca
     1381 gcctgcgccc gcgccgccaa accggcgcct gcgccaagca ccaacgtctc gttgcggcgg
     1441 cggtgaagca agcacgccac gtagcgttgc tgcccttcgc caatcgcggc gtcgactaaa
     1501 catcgaacta acgcatcggg gcatgggcac cagtccatgc cccgaattta tatccctgca
     1561 aaacacaaac aggggcaatc cctttattac aaagcgagta tcccatgacc aacgaatcgg
     1621 gcaaaaagcc cgctatgcat aaaaagcatg ttgcccgcct cgaacgcgag ctacagcaaa
     1681 cccgcctcat cctttacacc ttcttcggca tcctgggcgt ggtcgctgtc ttgctcatct
     1741 acggctggct cgatgtgaac tacctgcagt tgaatcgtgc ggttgccacc gtaaacgaca
     1801 acaagatcgt actcaaagat tttgaagccc gcacccgcct ccgcagacaa caactgatcg
     1861 acaactacaa ccaatacacc caataccagc aatacttcgg tatggacatg agctcacagc
     1921 tcaacagcat tgaaacccag ttgaacaccc ccgaattggt tggcacctct gtcttagacg
     1981 ccatgatcga tgaagagatc atccgcctcg aagcagaaaa acgcggcatc accgtcagtg
     2041 aagaagagat cgataagaaa atggaagaag gcttcaactt ctaccccaac ggcacgccca
     2101 ccccttctct cacccccact ccagccgacc tgcccaccga ccccgcagaa cttttgcaat
     2161 tcatcaccgc cacccccgat gtgaccgcta cgcctctctt cacggcgaca gctgagttta
     2221 cagccacggt tgaatcccag ccgacggcgg agggcgatgc gaccgctacc ttggcgccca
     2281 ccaacactgc cgaacccacc gtcaccccca ccgccacgct tggtcccaca tccaccccag
     2341 aacctacggc tactcccttc acccgcgaag cttatgaagt gttgcttggc aaaaccaaag
     2401 acaacattac caagtatggt ttcgatgaaa cctacattcg cgaattcttc cgcaaccaaa
     2461 ttttgcgcga aaaattgttg gaagtcatcg ccgcagatgt gaagcccttc gaagaacaag
     2521 tccgcgcgcg ccacattctc gttgccgatg aacaaaccgc caaagacctg atcgcccgcc
     2581 tgcaagccgg tgaagacttc gccgacctcg cccgccaata ctccagcgat agctcctctg
     2641 aacaaggcgg cgacctcggc tggtttggca aaggacagat ggttgctgaa tttgaaaccg
     2701 ccgctttcgc tttgcagaac cccggcgatt tcaccacaga acctgtcgct tcgcagttcg
     2761 gctatcacat catccaattg atggcgaagc aagaacgccc gctcagcgcc agcgaattgc
     2821 agaatgccaa agaaaccgct ctgcaagatt ggttgaccaa ggctcgcgac acagactaca
     2881 ccgttgaaac cttcgacttc tggaaacagc gcgttcccag caccccgaac ttcatcaccg
     2941 ctgccaccga gtctgctgcg gctcaaaaga ccgctcaagc tgagtctgtt gccacactcg
     3001 atgcaaagaa cagcgcggct gcaacaccca ccccgtaaag aacgatttca aataaaaaat
     3061 gctccagaaa atctggagca ttttttattt acagagcaag tcattcgccc aatattgcgc
     3121 cattcttccc tgcccctttc actttataca aacgcttatc tgcaaggtcc agcagtttat
     3181 agacattggc ttcttcactg aaacccgcaa accctacact gaccgtgact tcaatattca
     3241 atctctcgcc atgggtcata aacacgtggt tctgaattgc agttcgtatc ttttcgatcg
     3301 cgatcttcac gcccgatttt gacgtgtttg taaacaagac caaaaactcc tcgcccccat
     3361 agcggcaaaa aatatcgcgc ttaaagatcg cggacttgat gatacccgcc acttctgcga
     3421 tcaccatatc gccaacgaga tgcccataca tgtcattgat catcttgaaa tcatcaatgt
     3481 ctatgatcgc gatcccaaaa tttgtcttct tggcaggggc gtatcgggaa gggctttcca
     3541 aacgcttgag ttcgatatcc aaatactcgt tgaagaaacg ccggttatac gccccggtca
     3601 gcggatcgat ctcagacatt tcctgaagtt ttttcttgag gttctcgttc tcaataaaat
     3661 tatggaactg catgaacaag accgaaaaaa gaaccgcgcc tgaattgaac agcccgatcc
     3721 gctccacgag aaccgggttc aggtcataga gaggcgtaaa cgtcttcata tgaataaaac
     3781 tggcaacgaa tagcgtcata agagcggtca cttcaatcaa tttccgcctc aaagagagcg
     3841 ttaccgaatt gataataatg atggtggaag caattagata caggctgaaa taactttccc
     3901 atcccaaata aaaattggaa actaaagaag cgattgtgat tgccgtaatg ccaatatgca
     3961 tggcaagcaa atactttctc ttttggagaa ggtaaaaatt acccccaaac acaacaatat
     4021 tgattaaggc aaccaaaacc agctcggtgt ttttcaaata aagttgaaga acaacaaaaa
     4081 aagcattgat taccaagctt agagaaaaga atattcttaa aagagaatat tttcttttct
     4141 catctaaatc attcacattc atatcatcat actcaaactt tgttcgatta tatcccccca
     4201 cataaaaaac cgcccctaaa accaagaggc ggagaaaact cttacaattt tcaaattatt
     4261 ccttcacatt tcctacctcg aagcgacaat attcatcgcc catggcagta cagtgtgttt
     4321 cgataacttc ataatccttg ccggttgccc atttcaccgc ctcagaaacg gacccgatat
     4381 acaaatggca aatgggatgg tcactatggc gcgagttaca gattccgcaa gtagcatcga
     4441 cgtatgccag ccgttcaccg ctttcatcta cccaagcttt gacctgtgca ttgctcttct
     4501 tgagcgcatc tgccatgcca tttagaatga acttaatgcg ctgtttttcg ggcattaact
     4561 tgagtgccac accggcaacg cccaacaaag ctgcctgttc tcgtacccca tattgaaagg
     4621 atgctctgcc aatacgctgt aacatgcctt tgccgccgcg cccgtaaaaa tcttcaatgg
     4681 cttgattcaa ctgagcatat tgtgaggtct tgatcgcagg ctcaaggttg ttcggaggga
     4741 aattccctac aaaacgttcc aatccgcaag atttcaagac ggcgttcaaa ccgttatccc
     4801 ccatcacctc ctgcatggag gtcaacgcct gccgcacgag cgagttgata atgacggctt
     4861 cttctgggtt tatcatatgg gctcctatcg ttatggatca aagattcttt ctttagcata
     4921 ctcactattg aaagtatgtg taatgacctt atagtaccaa tcttaaactt ttcaaaaaga
     4981 cgttcttatc cctgcaaaca ccaaaaatag ggctcctttt cgaatggagc tttccaaaac
     5041 aaattaaaaa tcccatcacc aaagggccag gagccagaga tcgctccgtc ggtagggttt
     5101 ggaaagtcga tctgtaaaac ttgccgatag ccttgtgcga acaacgcctc acttcctggc
     5161 aggataggtt cttgaatact gtatggacgt ccgccaaatt tgtgccgctc acgaatcact
     5221 gtttcaccaa tatcattctg aatgaggtct gaagcaatat catggacctg aatggcatgt
     5281 ggactaagcc cagatgcata ttttgtatca gtactgcgtg gtttcttctc atggacgata
     5341 acaacaactc tatcgtcagt ttgaaaaccg gcattcaagg cgtggataaa gcttgcgaaa
     5401 gagccattaa taaaaatgga aaagaagagg cgggaatttc catcataaag cggaaaggtt
     5461 ccaaaatagc gggcatcgtc acggagaggg acggatattc cgtccggcat ggtaccgcca
     5521 attcttgcag ctgcgagaga agtctcttcg acaaaatgga acggacggga ttgctgaagt
     5581 tcgaatggga aaataaaatt ttcggtcatc atacgttcct tttcaaggaa ataatttctc
     5641 gcggacttca tccgcaagcc tgaaaacatc tgtcactatg cccatgccga tgggtggagc
     5701 gagatgagtt tggtctggtt tgtattgggg catattcccg agcagatgga attggttcgc
     5761 cgggtcgggc atgtcacgca tgccatagac aagatagtgc tgcacatgaa gaactccatc
     5821 tttaaatggg ataccgcgcg cctttttccc caagacgtcg tccctcacag ggcgataaga
     5881 cgtctccata tccatgaact ctacgatagg cgcttcattc gcgcctacaa tgccatggat
     5941 atacaaggca taaaattttc cccctatttg atacgccacc aaatctcctg tcctccaaaa
     6001 agtgagatct gccgggcggg cgggttttcg tgagcgaaga gcaaccggcg aggataggag
     6061 ttctttgatc gccgaagact ttctatagaa tgaatcactg agaattactt cccggttaag
     6121 cgcctcaata gcctgataag cttttgaatt aagagaaggg tcttgaattc catatttgtg
     6181 gagcaggagg gtcaacgtga ccacgatcaa attcttttgc ctcgcggtca cttcatcatc
     6241 agcggcagat aaagcacaat tccaccgaag cagaatattc ttaagaaccc gatccgctat
     6301 atcatcagaa ccgccaggcg aagcggacaa ctccctgcgg atcaagcgcg tcaggtcatg
     6361 gtcgagcgac gtcaccagtg agcgcgcgtc gagcaaatcc aaggcttgtt tgtatagtat
     6421 tgtcataccc ccaagttttc ttcaatttca tgcaatggtc aatcagacta atttcctaac
     6481 tccatcttat tgattaggga taatcttttc gatacaccag cacatcctga atcacttcaa
     6541 atccaatgga ctgatagggg tcgaacgcct tattgtgata attgtcagtt tcgataaaag
     6601 taacttcacc cctcatgact ttgttctatc ttttcttgac aacgacattc ctcgcaaggg
     6661 tcttaaatcg ttccccatcg gactgcacta ccgtaatagt gacttgaccc gtcttactcg
     6721 tcttaatatc gcggacggtt cccgatttgc ccgcatgcgt tcctttgaca acttcgcaat
     6781 gatcaccgtc tttaatttgt atagtctctg tcatgatact caatccttct acaagtactt
     6841 ctaataatct tttcgataaa ccaagacgtc ctgaatgact tcaaatccaa tggactgata
     6901 cagattaaac gcctcattgc gataattatc ggtttcgaca aaaatgtcct tcgcccccag
     6961 cgagcggaga cgattcaccc catgcgacag cgccactctt cccaacccaa gctgacggta
     7021 atccgcatgc acacccaacg gctcgacctg ccccgcggac agtttctcgt tgaaccagca
     7081 aatgcaaaac gccgccaact cccccgtcgg tgactcgacc acgagatcca actccggtct
     7141 ataaagcgga ctttgcagcg tgcgcccgcg ccattcaatc gtcatattct tgctctcaaa
     7201 cacggactgg tgcaaagcca catacttttc cacgtcccca cccagcgggc ggacagtgaa
     7261 cccggcaggc ggttcataga gcttcggctc cggtgaaagc ttcatgaaca cctttgacca
     7321 tgaatcctcg cccacgtcgg attgacaggc aaagcccatc tcttccaagt cttttttgcg
     7381 agactcttgc cccgagaaca cgttgacgaa ccaagccgaa cgctcgtacg gcgtaccggt
     7441 cacggcttga gcgcgtccat ccgcccatgc aaggatttcc ttgaacaagg ctggatagtc
     7501 cg
//
LOCUS       DAOQJE010000039         7476 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_5417_length_7476_cov_6.694920, whole genome shotgun sequence.
ACCESSION   DAOQJE010000039 DAOQJE010000000
VERSION     DAOQJE010000039.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 7476)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7476)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7476
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5417_length_7476_cov_6.694920"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..651
                     /locus_tag="PLT08_01860"
     CDS             <1..651
                     /locus_tag="PLT08_01860"
                     /inference="COORDINATES: protein motif:HMM:NF013100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HNE03235.1"
                     /translation="SSQMGANTFYKYMDAFGIGHLTGVDLAWEAPGRLKVPGDADWYP
                     VDLGTNSFGQGVAVTPIQMMSAVSAVANNGRMVIPHVLYAMVRDGRQYEVPDQFGPTP
                     ISEQTSRTLSEMLAVSLESESSAALIPGYRVAGKTGTAQIPVDGFYDSTQTNTSFIGW
                     GPVDDPKFMIYVWLERPTASPWGSETAAPVFAEMAQRTVVLLDIPPDSIRLQVAQK"
     gene            653..2071
                     /gene="murF"
                     /locus_tag="PLT08_01865"
     CDS             653..2071
                     /gene="murF"
                     /locus_tag="PLT08_01865"
                     /inference="COORDINATES: protein motif:HMM:TIGR01143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047480 -
                     UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HNE03236.1"
                     /translation="MLTLADVLEALTTIRPANASTIITEGAIDSRQVIPGSLFVAIPG
                     EKTDGHDYLTDAFKRGASFALIQRDVDASFRTLDLRAGLSTDATLNFESPLCLRVDNT
                     ISALQQIARFWRRKLPNLRVVGITGSVGKSTTKETVAEVLSLRYRTLKSPGNLNNEIG
                     LPLTILRLGTGYDRAVLEMGFYVPGEIQFLCDIAQPQMGVVSNVGTVHAERAGSQEAI
                     FRGKSELPASLPADGVAILNFDDQWVRKMEEKTKARVFFYGLSPEAHLWADQIEGLGL
                     DGIRFRMHYGKEIIHMRVPMIGRHSIHTALRAAAVGLNDGISWQEILEGLSHGHTQLR
                     LVAARNETGALILDDTYNAAPESMLAALNLLDELDGRKIAVLGDMLELGPYERQGHEM
                     VGMRAAQVAKVLITLGTRGHMIAEAARRAGMKASAIMEYEDIAPIIDWLTKNLNSNDA
                     VLIKGSHGLRMDRIAAALEVRS"
     assembly_gap    2205..2304
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            2452..3825
                     /gene="murD"
                     /locus_tag="PLT08_01870"
     CDS             2452..3825
                     /gene="murD"
                     /locus_tag="PLT08_01870"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008764 -
                     UDP-N-acetylmuramoylalanine-D-glutamate ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HNE03237.1"
                     /translation="MTNWTGKHVLILGAARQGLALARWLARHGANVTLNDSRREEDLI
                     TAKKSLADVNVTWAVGGHPLELLDTTDVLCISGGVPLTLPIVEEAIKRDIPLSNDSQI
                     FMEVVSCKTVGITGSAGKTTTTTLVGNMAKLAKGDRAFVGGNIGDPLINYVDDMQADD
                     LAILELSSFQLEQMTISPNIAAILNITPNHLDRHGTMEAYTNAKAHILDFQSAKDNAI
                     LGRDDKGAWGLKNRVKGNLLSFSLQDLDEGLNGTYLHDGLLSLRDGFAYVPLLMREKV
                     QLRGDHNIANVLAAFAIGHAAGFKLDDMLEAVEEFRGVPHRLELVRELNGARWYNDSI
                     ATAPERAAAAVHAFSEPIVLMLGGRDKNLPWGDLAKLIHERVDHVVLFGEAGEMIQNA
                     ITAYSGVGTVDVHRATTLKEAVTLAADVASAGDVVLLSPGGTSFDEFKDFAERGEAFR
                     KWVSELS"
     gene            3804..5045
                     /locus_tag="PLT08_01875"
     CDS             3804..5045
                     /locus_tag="PLT08_01875"
                     /inference="COORDINATES: protein motif:HMM:NF013279.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative peptidoglycan glycosyltransferase FtsW"
                     /protein_id="HNE03238.1"
                     /translation="MGIGTFVRETSPQDRPRAGGFDMPLLVIVVALIVFGLVMLYSAS
                     WDFSRAAYNGDAMYMFNRQLMWLGLGTVAALILAFIDYHRWRQFIVPAMAFTVLMLII
                     VLVMSEVRFNATRTIFNGSIQPSELAKLVSILYLAVWLYAKREMLKDITFGLIPLGVI
                     LGFVGGLIYLQPDLSAAATVLMLGGLLFFLAGGDLKQIGGLLIIAFAVAWLVVSISLT
                     GQQRVSDFVAGIKDPLQASYHVRRSFEAIVNGGWFGVGIGKSLSKVTGLPVPPTDSIY
                     AVVVEELGWFGGVGLIGLFGALVWRGLVIARRAPDMLGTLLASGLVIWIGLEALINMA
                     VMVGLLPFAGNALPFVSAGGSNLVTTLAALGILFNISRQGSSANETNDEWRSYSAVAN
                     LRWWNGRGRVSRSVRLRSAKE"
     gene            5005..6063
                     /locus_tag="PLT08_01880"
     CDS             5005..6063
                     /locus_tag="PLT08_01880"
                     /inference="COORDINATES: protein motif:HMM:TIGR01133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine--N-acetylmuramyl-
                     (pentapeptide) pyrophosphoryl-undecaprenol
                     N-acetylglucosamine transferase"
                     /protein_id="HNE03239.1"
                     /translation="MYPALSVYEALKSKHPNVETLWVGGEGGMEEDLVKRAGIPYRSV
                     PAAGVHGVGLRALPGNFTKLTRGVLESRRILKEFKPDVLFFTGGYVAGPMAVAGRNLP
                     IVLYVPDIGPGLALKFLGYFADVITVTAPDSKKFFSHAERIVHTGYPLRSGLLNWSRE
                     KAISHFQLDSSLPTLLVTGGSKGARSINMAVLKSLNELLEMTQIIHLTGSLDIETAQQ
                     AAANLSAEKKLRYHALPYLHDMGAALSAADLVLSRAGASSIGEDPFFGLSAVLTPYPY
                     AWRYQKVNADYLAERNAAIILQDELLNDNLLTVIKDVLNNAQKRQAMRTAMKNLSMPN
                     AADTIASQLVKLAGDETL"
     gene            6060..6608
                     /locus_tag="PLT08_01885"
     CDS             6060..6608
                     /locus_tag="PLT08_01885"
                     /inference="COORDINATES: protein motif:HMM:NF014704.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009403 - toxin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HNE03240.1"
                     /translation="MMSAVYIFWMYVFLFGIIGAMRGWAKELMVIFSVVTSLAVNLLL
                     EKYIPLTRNLVDPNNPTQDALTAQFWIRTLILTALVYFGYQTVNISRFAPKAARERLQ
                     DSMFGAVLGGVNGYLVAGSVLYYNHVANYPFRNVISPATDAAIADAINRMMNVMPPRF
                     LAEPQIYFAVIIILIFIIVVYI"
     gene            6630..>7476
                     /locus_tag="PLT08_01890"
     CDS             6630..>7476
                     /locus_tag="PLT08_01890"
                     /inference="COORDINATES: protein motif:HMM:NF019850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase domain-containing protein"
                     /protein_id="HNE03241.1"
                     /translation="MTHVHFIGIGGSGLSAIARLLKESGYEVTGSDQSLSPFAAELQS
                     IGIQVFVGHHPRNVSGAEMVIRSSAIKDENPEVKAALQAGIPVYKRSDFLGRLMENKI
                     GIGIAGTHGKTTTTAMVAWMLYSLNRDPSFIVGGTLNNLKVNARAGKGDTFVIEADEY
                     DRMFLGLKPQIEVVTNLEHDHPDCFPTFADMYSAFESYIDLLPPDGTFITCAEDDGAA
                     TLISHARRKGLSVVSYSVQGEMTINSPQWVQARTMHPNEKGGFDFSVMTNMGSMTNAF
                     KVSLQV"
BASE COUNT         1652 a         1747 c         1950 g         2027 t
ORIGIN      
        1 tcttcacaaa tgggtgcaaa tactttctat aaatacatgg acgctttcgg aattggtcac
       61 ctcaccggcg tggacttggc ttgggaagcg ccaggcaggc tcaaggtccc gggagatgcg
      121 gattggtatc ccgtggatct tggtacaaac tcatttggtc aaggtgtagc agtcactccc
      181 atccaaatga tgtctgccgt ttctgcagtt gccaataacg gacgcatggt catcccacat
      241 gttttgtatg ccatggtgcg cgatggtcgc caatacgaag tgccagatca gttcggtcct
      301 actcccatct cagaacagac ctctcgtaca ttatctgaaa tgctagcggt ttctctcgaa
      361 tcagaatcat ctgctgcatt gatccctggg tatcgcgtgg ctggtaagac cggtacagcg
      421 caaattccag tcgatggttt ttacgacagc acgcaaacaa atacctcttt catcggttgg
      481 gggccagtag atgacccgaa attcatgatc tatgtttggc tcgaacgccc tacagcttca
      541 ccttggggtt ctgaaactgc tgcccctgtt tttgctgaaa tggcgcagcg aaccgtggtc
      601 ttgttagata tccctcctga ttcaattcgc ctacaagttg ctcaaaaata atatgcttac
      661 tctagcagac gttcttgaag ctcttaccac tattcgccct gcaaatgcct ctacaatcat
      721 tacagaaggt gccatcgatt cgcgccaagt gattccgggt tctctttttg ttgccattcc
      781 cggcgaaaaa acagatggac atgactatct gacagatgcc ttcaagaggg gggcttcttt
      841 tgctttaatc caacgagacg tagacgcctc tttccggacc ctcgacttgc gtgctggtct
      901 atcaactgat gccacactga actttgagtc tcctctctgc ctgcgcgtgg acaatacaat
      961 ttcagcctta caacaaatcg cgcgtttctg gcgccgtaaa ttgccgaacc tgcgcgtggt
     1021 aggaatcaca ggaagtgtgg gtaaatctac aaccaaagaa actgtagcag aggtgttaag
     1081 ccttcgttac cgcacactca aaagccctgg caatttaaat aacgagattg gacttccgct
     1141 caccatatta agactcggta caggttatga ccgtgctgtg cttgaaatgg gattttatgt
     1201 acctggcgag attcaatttc tttgtgatat tgctcagccg caaatgggtg ttgtttcaaa
     1261 tgtaggtact gtccatgcgg aacgcgccgg gtcacaggaa gctattttcc gcggcaagag
     1321 cgaattacca gcctctcttc cagctgatgg ggttgcgatt ctaaattttg atgaccagtg
     1381 ggtccgcaaa atggaagaga agaccaaagc tcgcgttttc ttttatgggc tttctccaga
     1441 agcgcatttg tgggcagacc agatcgaagg gctgggctta gatggcatcc gcttccgtat
     1501 gcattacggt aaagagatta tccacatgcg cgtgccaatg attgggcgtc attctattca
     1561 tacggcatta cgcgctgctg ctgttgggct gaatgatggc atttcttggc aagaaatttt
     1621 agaaggtctc tcccatgggc acactcaact gcgcttggta gctgctcgta atgagaccgg
     1681 cgctttgatt ttggatgata catacaatgc tgcgcctgag tctatgttgg cagcgttaaa
     1741 tctgctagac gaattagacg gtcgcaagat tgccgtgtta ggtgatatgc ttgaacttgg
     1801 tccctacgaa cgccagggac atgaaatggt cggaatgcgt gcggctcaag tggcgaaagt
     1861 cctcattacc ttgggcaccc gtggacacat gattgcggaa gcggctcgtc gagccggtat
     1921 gaaggcttct gctattatgg aatacgaaga cattgccccg atcatagatt ggttgacaaa
     1981 gaatttgaat tcaaatgacg cagtgttaat taaagggtct catggcttac gcatggatcg
     2041 catcgctgct gcattggagg tacgctcgtg agagacattg ctctttccct aagcctggcg
     2101 gggttggctt ttatcatgac agccatttgg ggaaatccgt tgctgcgtat tttgcggcac
     2161 tttaagttgg ggaagatcat ccgtattgaa cagccggaag gtcannnnnn nnnnnnnnnn
     2221 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2281 nnnnnnnnnn nnnnnnnnnn nnnntgacag gcggaaagcg ctttttcaaa atgactccca
     2341 tccatcatca ttttgagttg ctgggttgga gtgaaacgca aattgtccaa cgcttttggc
     2401 tcatcggctt gatggcagcc ttggttggaa tcggattatc gcaagtgtag tatgacgaat
     2461 tggactggta agcacgttct cattcttgga gccgcccggc agggactcgc ccttgctcgt
     2521 tggcttgcac gtcacggcgc aaacgtgacc ttgaatgatt cgcgtcgcga agaagacctt
     2581 atcactgcga aaaaatctct cgctgatgtg aatgtgactt gggcagtggg tgggcatccg
     2641 cttgagttat tggatacaac tgatgtattg tgtatctctg gtggcgtacc gttgaccttg
     2701 cctattgtag aagaagctat taagcgtgat attccgcttt cgaatgactc acaaatcttc
     2761 atggaagtgg tttcatgcaa aacggttggc atcactggtt cggcaggtaa gacgaccaca
     2821 acaactctcg ttggcaacat ggcaaaactt gcaaaaggcg accgtgcttt tgtgggtggc
     2881 aatattggcg atccactcat caactatgtg gatgacatgc aagctgatga tttagccata
     2941 ttggaacttt cttccttcca acttgagcag atgactattt caccgaacat tgcggcgatt
     3001 ttgaacatca caccgaatca cttagatcgc cacggtacga tggaagccta taccaatgcc
     3061 aaggcgcata tcttggattt tcagtctgcg aaggataacg cgatcttagg gcgcgacgat
     3121 aaaggcgctt ggggtttgaa gaaccgtgtg aaagggaatt tactttcgtt cagcttacag
     3181 gacctagacg aaggcttgaa cggtacatat cttcacgatg gtttgcttag cttacgagat
     3241 gggtttgctt acgtcccatt gttgatgcgt gaaaaagttc agctacgtgg cgaccataac
     3301 attgcaaatg tgttggctgc ttttgccatt gggcacgcgg ctggtttcaa gttggatgat
     3361 atgctcgaag ctgtggaaga attccgtggt gtgccgcatc gcttggagtt ggtgcgcgaa
     3421 ttgaatggtg cacgctggta taacgactcg attgctacag ctcccgagcg cgcagcagca
     3481 gccgtccatg catttagtga gccgatcgtg ctcatgcttg gtggacgtga caaaaatctt
     3541 ccctggggcg atcttgccaa acttatccac gaacgtgtgg accatgtggt gctctttggt
     3601 gaggcggggg agatgattca gaacgcaatt acagcctaca gcggagtcgg aaccgtggat
     3661 gttcatcggg caacgacact caaagaggcg gttacactgg cagcggatgt tgcatccgca
     3721 ggggatgtcg tccttttatc accgggcggc acgagctttg atgagtttaa ggattttgcg
     3781 gagagaggag aagcgtttag aaaatgggta tcggaacttt cgtaagagaa acttcaccgc
     3841 aagaccgtcc gcgtgcgggc ggcttcgaca tgccactctt ggtgatcgtg gtggcattga
     3901 tcgtatttgg tttggtgatg ttgtactcag cttcttggga cttttcacgc gctgcctata
     3961 acggcgatgc gatgtatatg ttcaatcgtc agttgatgtg gcttgggctt ggaaccgtag
     4021 ccgcgctgat tttagcgttt atcgattatc accgttggcg ccaattcatt gtgcctgcta
     4081 tggcgttcac tgttttgatg ttgatcatcg tgttggtaat gagcgaagtg cgtttcaacg
     4141 ctacgcgtac catcttcaat ggttcgatcc aaccttctga gttggcaaag ttggtatcga
     4201 tcttgtatct tgcggtttgg ttatatgcca agcgcgaaat gctgaaagac attacatttg
     4261 gtttgattcc cttgggtgtc atcctcggtt ttgtgggcgg cttgatctac ctgcaaccag
     4321 acttaagtgc tgcggcaact gtgcttatgc tcggcggttt gctcttcttc ctcgcaggtg
     4381 gtgacctgaa acagatcggc ggtttgttga tcattgcttt tgccgtggcg tggttggtgg
     4441 tttcaatcag ccttacgggt caacaacgcg tttctgattt tgtcgcagga atcaaagacc
     4501 cattgcaggc ttcctatcat gtgcgtcgtt cgtttgaagc catcgtcaac ggcggatggt
     4561 tcggtgtagg gatcggtaaa tcgctttcca aagtcactgg cttgcccgtg cccccgacag
     4621 atagtattta cgctgttgtt gtggaagaac tggggtggtt cggcggcgta ggcttgatcg
     4681 gtttatttgg tgcgctcgtc tggcgcggac ttgtcatcgc tcgtcgtgcg cccgatatgc
     4741 ttggaacatt gcttgcttct ggcttggtca tttggatcgg cttggaagcg cttatcaata
     4801 tggcagtgat ggtggggctg ctgccgtttg caggaaacgc tcttccgttc gtcagtgctg
     4861 gtggctcgaa cttggtcacc accttggcgg cgttgggaat tctcttcaac atttcccggc
     4921 agggaagtag cgcgaacgaa acgaacgatg aatggaggtc gtacagtgcg gttgctaatt
     4981 tgcgctggtg gaacgggcgg gggcgtgtat cccgctctgt ccgtttacga agcgctaaag
     5041 agtaagcatc caaacgtcga aactctttgg gttggcggcg aaggcggcat ggaagaagac
     5101 ctggtgaaac gtgcaggcat tccataccgt tctgttcctg ctgccggagt gcatggcgtg
     5161 ggtctgcgtg cattgcctgg caactttacc aaactcacac gcggtgtgct tgaatcacgt
     5221 cgcatcctga aagaattcaa acccgatgtt ttgtttttca ccggcggcta tgttgcgggt
     5281 cccatggcag tagctgggcg caacttaccc atcgttttgt acgtacctga tattgggcca
     5341 gggcttgcgc ttaaattcct gggctatttt gcggatgtca tcaccgtgac cgcgccggac
     5401 tcaaagaagt ttttctcaca cgcagagcgc atcgtccaca ccggctaccc gcttcgttcc
     5461 ggacttttga actggtctcg cgaaaaagcc atttctcact tccagctgga ttcttccctt
     5521 cccaccctct tggtgactgg cggtagcaaa ggcgcacgtt cgatcaacat ggctgtgttg
     5581 aaatctttga acgaattact agagatgacg cagatcattc acctcactgg ctcgttggat
     5641 attgaaaccg cacaacaagc cgcagcgaat ttatctgcag agaaaaagct ccgctatcat
     5701 gccctgccct atttgcatga tatgggtgca gccctctccg ccgcagactt ggtcttgtct
     5761 cgagctggtg cctcttcgat aggggaggat cccttctttg ggttgtctgc cgtgcttacc
     5821 ccctatccgt atgcatggcg ctatcagaag gtcaatgctg attatttggc agaaagaaac
     5881 gctgcgatta tcttgcaaga tgaattgttg aacgataatc tcttgacggt cattaaagat
     5941 gtattgaaca atgcgcaaaa gcgccaagcc atgcgtacag cgatgaaaaa tttatccatg
     6001 cccaatgcgg cagacaccat tgccagccag ttggtaaaac tggcaggaga cgaaacctta
     6061 tgatgagtgc tgtttatata ttctggatgt atgttttcct ctttggcatc atcggcgcca
     6121 tgcgcggatg ggcgaaagaa ttgatggtca tcttcagcgt ggtcacttcg ttggcggtta
     6181 acttgttgtt ggaaaaatac attccactga cccgcaacct tgtagacccc aacaacccaa
     6241 cccaagatgc gctcaccgcc cagttttgga ttcgcacctt gatcctgacg gcgttggtgt
     6301 actttgggta tcaaaccgtg aatatctcac gctttgcccc gaaggcagcc cgtgaacgct
     6361 tacaagactc catgtttggt gcggtcttgg gcggtgtgaa cggatatttg gtggctggct
     6421 ctgttcttta ttacaaccat gtcgcgaact atccctttcg caatgtgatc agcccggcaa
     6481 ccgatgccgc aattgcggat gcgatcaacc gtatgatgaa tgtgatgccg ccgcgctttc
     6541 tcgcagaacc gcagatctat tttgcggtca tcattatttt gatcttcatc attgttgtgt
     6601 atatctaata ctttctgctg gattacttta tgactcatgt tcatttcatc ggcattggag
     6661 gttcggggct ttctgctatc gcgcgcctgc tcaaagagag cggctacgaa gtgaccggct
     6721 cagaccaatc tctttctcct tttgcggctg aattgcagag tattggtatt caggtttttg
     6781 tggggcatca cccacggaat gtttccggcg cagaaatggt catccgctct tctgccatca
     6841 aggatgagaa tccggaggtc aaggcggcat tgcaggcagg tatccccgtc tacaaacgct
     6901 ccgattttct tgggcgcttg atggagaaca aaatcggtat tggtattgcc ggcacccacg
     6961 gcaagactac tacaaccgcc atggttgcat ggatgttgta ttcgctcaat cgtgaccctt
     7021 cctttattgt gggcggcacc ttgaacaatc tcaaggtcaa tgcgcgggca ggcaaaggcg
     7081 acacgtttgt gatcgaagca gatgagtatg accgcatgtt ccttgggctc aaaccacaga
     7141 tcgaagtggt taccaatttg gagcacgatc accccgattg cttcccaacc tttgcagaca
     7201 tgtactctgc cttcgaaagt tacatcgacc ttctgccgcc agatggcaca tttatcacct
     7261 gtgcagaaga tgatggcgcc gcgacgttga tctcgcatgc ccgccgtaaa ggcttgagcg
     7321 tagtttcgta ttctgttcaa ggtgagatga ccatcaacag cccgcaatgg gtgcaagctc
     7381 gtacgatgca cccgaacgaa aaaggtggct ttgatttttc agtgatgacc aatatgggca
     7441 gcatgaccaa tgctttcaaa gtgtctttgc aggtgc
//
LOCUS       DAOQJE010000040         7476 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_5418_length_7476_cov_6.439968, whole genome shotgun sequence.
ACCESSION   DAOQJE010000040 DAOQJE010000000
VERSION     DAOQJE010000040.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 7476)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7476)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7476
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5418_length_7476_cov_6.439968"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(64..2751)
                     /locus_tag="PLT08_01895"
     CDS             complement(64..2751)
                     /locus_tag="PLT08_01895"
                     /inference="COORDINATES: protein motif:HMM:NF013124.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNE03242.1"
                     /translation="MANKIPFVDRKKELDLIEKHIVKSKGKHILWIQGPGGSGKTRLL
                     EEVLLRYGYLKKNGYEFSPIVDFDDRSLHIPENLDRMLVQWLGEKPLKQYLRETLDRQ
                     KMEEAGVSEETLLRQSDHVTQWLIETFNQSSKKKRRILLFDTVEKVEDKVWQHICDLL
                     QHAENTFILLVSRKNDRLLDTLNSKITKSIEIIQWEPLEDEKFGELYLDQKQRSLHVA
                     LPPSICKKLVLLVGGRPILIDLTVEAISRNLMPEILTHKTYAEIKALSSRKSGKLRQD
                     FETQLVRQIMDVRDSMDRLVLVMSRVYPLSQKMVTEFLNITERDAKNLFEEAKLKTTI
                     KILPDGRISLHDEIRTMVENYVWPYIDPDGDRQKRDSRIAIDYLRHEEVEIQKDLKKL
                     EDQASVEKTQDAGISLDVMSKMESLKQYLWDVREQLLGHSLYINTEEGIQTFADIFDR
                     ATRAYHFPYREVLLRKVEPYMPHLPVALKYEVNSRKVKASLDTGNYIEADRLTTQVLK
                     DKSIAPQQRVDMLIQHGNIKIRLGKLSEGKEKFEKAVRICKAGNDIRNLVPALNARGW
                     GYRQVGNIDKAITDYQNALELSFSTGDELRRAWILNNLAYVYSQQGRFNPALALCEQA
                     KELWKKIGFGRGMGALYEVYGHIYIKMGRYDEAMEYFQLALDEFKSRDVEWQSRIHAG
                     LGFVYRMMKDRKNAERELKLALDYRIQKDRAMILHRMAHLQLEGGKIKKAKNLFEQSY
                     EASREISDAYHELNNLGDLAKLAVGEKDFSNAARFEKLYQEYKTTWPDMTYSRVEGMI
                     LRHLGDLMLGNPKNNLSKAITYYQKAFLLLARYETYKEYSVQKQLEILDAYFHELKVP
                     ANVKSKIGQSLYFVWQKNNLIEHHPEALGFFAQWGGTVK"
     gene            complement(2762..4705)
                     /locus_tag="PLT08_01900"
     CDS             complement(2762..4705)
                     /locus_tag="PLT08_01900"
                     /inference="COORDINATES: protein motif:HMM:NF025056.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003953 - NAD+ nucleosidase activity
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="toll/interleukin-1 receptor domain-containing
                     protein"
                     /protein_id="HNE03243.1"
                     /translation="MTKKQLRVFLCSGKEAQEHVRLLHRRLCADGIDCILDEEIPIGG
                     QNWKVEIRKKIKSSDIVIICLLKKTIAKEGFIHEEITLALEADKEKPDGTIYLIPAKF
                     ERCSLPAPMKEKQAVDLFTAKGYEKLTNALKVRANELKRAFPEHVSKGPLFEFDGQIE
                     FSKENTITAGSNFSSVMFVNRVDEIEAVTATASSGGQYFLVDAPAGYGKTWFMHKLDE
                     YFAVKKNWLHAMVSVKKDCNLEVLAKELAKKLGLELRSGQGSDWGFRLSGAIKKRYST
                     DTMPAGLVLLIDLDGDPSFLLAQDILEKFIPQIWEILSDLDAFSRSATPSLFRVILSG
                     RNITTEKSIKIYRDQLPFINKILSPFTYDVVYKSAKNHLPQIEKKKLEQLVAHLLFLT
                     GGHPGCMSSVLDLYKSGDIPIERFVTDTQQAIWDQIVRDVTEKVWTGLNYRDKKLQEA
                     IHRLSVYRYVNNHVLRYVIENCPVKGMRDEIDLRDMLTGSYLYTNDGRFLQDGIIRQL
                     VLIHLRQTMPKLYEGYCQDAYQVCKDQLTSPNVQLPEKWIVEMLNQCLLQHAGNGIEQ
                     PAFRSKISKQFNKEVMSIMQVRKKTKSSLSDWREEVDALLSEMDKEPQWEFRFSVNYF
                     LRDGQYTDEPFQKLKAEIKKQLK"
     gene            complement(4821..5711)
                     /locus_tag="PLT08_01905"
     CDS             complement(4821..5711)
                     /locus_tag="PLT08_01905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE03244.1"
                     /translation="MKRAVGISLGSSKRDKKVIVNLNGVEIQVERIGTDGDIEKARRL
                     YLELDGKVDAFGVGGVDLYLRLDAREYPLHAALKLVSGVKQTPLCDGRGLKHTLERRV
                     FQLAKAELGEVRFKQAFIPVAVDRMGLAEAVAEVSDQIVSGDLMVALGVPIPLHGIPA
                     FKRVARVMLPMVSYFPMSMIFYGSDGAEQEPKYVKYFEESDLIAGDFLFMRKYMPKSL
                     AGKTVVTNTTTEENIALLKERGVKTVITTTPRYDGRSFGTNTTEAMLTAYADKGRRLT
                     DEELNGLIDELGLKPSVISL"
     gene            complement(5730..6365)
                     /locus_tag="PLT08_01910"
     CDS             complement(5730..6365)
                     /locus_tag="PLT08_01910"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HNE03245.1"
                     /translation="MLEIVQFTLGPASTNAYLVADPETKEAVVIDPSWDGHLILAEAQ
                     KRGWRIGHLWYTHAHFDHIGGAAAIADAMNPLPHVALHPDDHVLWREGGGGKKFGLDI
                     DPGPEPTIDLIHGMTMKLGSNDFQVRFTPGHTRGHCVLYVAKESICFCGDLIFNESVG
                     RTDLPGSSFSALEKSIREQIYTMPDETVLLSGHGPKTTVGHEKKNNPFVSE"
     gene            complement(6358..>7476)
                     /locus_tag="PLT08_01915"
     CDS             complement(6358..>7476)
                     /locus_tag="PLT08_01915"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNE03246.1"
                     /translation="NLSFIAAALLLWGFGEGMFFNFVPIYLDVQFLLSKYEIGLILGG
                     FGFFMAITHIPAGRLADRLGRKPLLIVAWLLGLLSTLTMGSAKTLPFFLIGLFTYGLT
                     AFVSSPLSSYVTAARGQLPVATVLSLTTATFGMGMVLGPVTSGWIGEHYGMRMSFYAA
                     AVVFVFSNIFVYFIGSQPIDHHDPSAPPLGLGSNKKFVTLMFVLAFAIFSMYLAQPLT
                     PNFLEGVRGLSLSQTGLIFTIGALGNSLMAITFSRVNPRYGFLAAQSLVILFAVLIWY
                     GTSLPFFALGYFLLGGFRAGRPMALAQARELVHESQMGVTYGVMETVSAIITIITPPI
                     AGFLFEFDPFIVYPISIGLLVLSILLSSVFLPRKESHA"
BASE COUNT         1984 a         1720 c         1538 g         2234 t
ORIGIN      
        1 gccacgggcc gatggtcaaa gataacactg ggtgtttctt gggttgcaaa tttattaaac
       61 atgttattta actgtgcctc cccattgcgc aaagaaacca agcgcttctg gatgatgttc
      121 tattagattg tttttctgcc aaacgaaata gagagattgc ccgatttttg attttacatt
      181 tgctggtact tttaattcat ggaagtacgc atcaagtatt tcaagttgtt tttgtacaga
      241 gtactcttta taagtctcgt accgtgctag caacaagaag gctttttgat agtaagtaat
      301 tgctttagat aggttgtttt ttggattgcc caacataaga tcgcccaaat gtctcaggat
      361 cattccttcc acacgcgagt aggtcatgtc tggccaggtt gttttgtatt cctgatatag
      421 cttttcaaat ctcgctgcgt ttgagaaatc cttctcacct acagcgagtt tggcaaggtc
      481 tcccagatta ttcagttcat gatatgcatc agatatttcg cggctggctt cataactttg
      541 ttcaaaaaga ttttttgctt tctttatttt tccgccttcc aattgaagat gcgccatacg
      601 gtgcaagatc attgcccgat ctttttgaat acgatagtct aaagcgagtt tcaattcacg
      661 ttctgcattt ttgcgatcct tcatcattct atatacaaac cccagcccag cgtgaattct
      721 gctttgccat tcgacatcac gagatttgaa ctcatctagg gctagctgaa agtattccat
      781 ggcttcatca tagcgtccca ttttgatgta gatatgccca tatacttcat acaatgcgcc
      841 cataccacgc ccaaaaccga ttttcttcca aagttccttt gcctgttcgc aaagagctag
      901 cgccgggttg aagcgtcctt gctgactgta tacatatgcc aaattgttca gtatccatgc
      961 acgtcgcaat tcatcgccag tactaaaact tagttccaat gcattttgat agtcagtaat
     1021 cgctttatct atattcccaa cttggcgata cccccagcct ctcgcattca aagcagggac
     1081 aaggttacga atatcattgc ctgctttgca gatacgaaca gctttctcga acttttcttt
     1141 cccctctgat aactttccca atcggatctt tatatttcca tgctggataa gcatatctac
     1201 acgttgttga ggagcaatag atttgtcttt cagtacttgt gttgtaagtc tgtctgcttc
     1261 gatgtagttg ccagtatcta gtgacgcctt aactttacgg ctattaactt cgtacttaag
     1321 cgctaccgga aggtgcggca tgtatggttc aactttccgt aataagacct cacgatatgg
     1381 gaagtgatat gcgcgcgttg cccggtcaaa gatatctgca aatgtttgaa ttccttcttc
     1441 cgtgttgata tacaacgaat gtccaagaag ctgttcgcgg acgtcccata aatattgttt
     1501 tagagattcc atctttgaca tcacatcaag cgaaataccg gcgtcttgag ttttctctac
     1561 agaagcctga tcttcaagct tttttaggtc tttttggatt tcgacttctt cgtgccgcaa
     1621 ataatctatc gcaattcggc tatcgcgttt ctgccggtca ccatccggat caatgtatgg
     1681 ccaaacataa ttttcgacca tggttctgat ctcatcgtga agagatattc ttccatcggg
     1741 caatattttt atcgttgtct ttagttttgc ctcctcaaaa agatttttgg catctctttc
     1801 cgtgatattg aggaattctg tgaccatttt ctgcgacaat ggataaactc gcgacatgac
     1861 caaaaccagc ctatccatag aatcacgtac gtccatgatc tgtcgtacta attgagtttc
     1921 aaaatcttgt cgcagtttcc cactcttccg gctggaaagg gcttttatct ctgcataggt
     1981 tttatgtgtc agaatctctg gcattaaatt acgagaaatt gcttctacgg tcaaatcaat
     2041 aaggatgggt cttccgccaa ccaagagtac aagtttttta catatgcttg gcggtagggc
     2101 tacatgtaag gaacgttgct tctgatcgag atataactcc ccaaactttt catcttccaa
     2161 tggctcccac tggataatct caatactttt cgtgattttg ctatttaatg tgtcaaggag
     2221 gcgatcattt tttctgctga ccagcaaaat gaatgtattt tcggcatgtt ggagcaggtc
     2281 acaaatatgt tgccaaactt tatcctccac tttttcgaca gtgtcgaata ataaaattcg
     2341 gcgtttcttt tttgacgatt ggttgaaggt ctctattaac cattgtgtaa cgtggtcact
     2401 ttggcgaaga agagtttctt cactgacccc tgcctcttcc attttttgtc tatccaacgt
     2461 ttctcgcaga tattgtttta gaggtttttc tcccagccat tgaactagca tgcggtcaag
     2521 gttttctgga atatgaagtg aacggtcatc gaaatcgacg atcgggctga attcatatcc
     2581 gttctttttt aagtatccat agcgtaacag cacttcttcc agcaaacgtg tcttaccgct
     2641 accccctggt ccctggatcc ataaaatatg ttttcccttc gatttcacga tgtgcttttc
     2701 aataagatca agttcttttt ttcgatcaac gaaaggaatt ttatttgcca tgacacacct
     2761 cctatttcaa ctgcttctta atctcagcct tcaacttttg gaagggttca tcggtgtact
     2821 gcccatcgcg caagaaatag ttaaccgaga agcgaaattc ccattgaggt tctttgtcca
     2881 tttcagataa caaggcgtcg acctcttctc gccaatctga aagcgatgat tttgtttttt
     2941 tacgaacctg cattatcgac ataacttctt tattaaattg cttggatatc tttgatcgaa
     3001 aggcaggctg ttcaattccg ttccctgcat gttgtaatag acattgattt agcatctcaa
     3061 cgatccactt ttcaggaagt tgaacgttag gagaagtcaa ttgatccttg caaacttgat
     3121 acgcatcttg gcagtacccc tcgtatagtt tcggcattgt ttggcgcaga tggatcaaga
     3181 ctagttgccg tatgatgcca tcttgcaaaa aacgtccatc gtttgtatat aaataactgc
     3241 ctgttagcat atcacgcaga tcaatttcat cgcgcattcc ttttacggga cagttttcaa
     3301 ttacatatcg taaaacgtgg ttgtttacat accgatacac acttaagcga tggattgcct
     3361 cttgcagttt tttatctcgg tagtttagcc ctgtccaaac cttttcagtt acatcgcgaa
     3421 caatttgatc ccatattgct tgctgagtat cagtcacaaa tcgctcgatg ggaatatcac
     3481 ccgatttata aagatctagc acagaactca tacatcctgg gtgccctccc gtgaggaaca
     3541 agagatgagc tacaagttgc tctaatttct tcttttctat ctgtggtaaa tggttttttg
     3601 cagacttgta caccacatca tatgtaaatg gggaaagaat cttgttgata aatggaagtt
     3661 gatcgcggta tatctttatg cttttttcgg ttgtaatatt cctgccagaa agaatgaccc
     3721 gaaacaagct tggagttgca gaacgcgaaa aggcatccag gtcagataaa atctcccaaa
     3781 tttgagggat aaatttttcc aagatatcct gtgctaataa aaaagatgga tctccatcca
     3841 ggtcaattag taataccaga ccagctggca tagtatctgt ggaatatcgt tttttgatcg
     3901 caccacttaa acgaaacccc caatcagagc cttgtcctga tcttaattcc aatcctagtt
     3961 tttttgctaa ttctttggct aatacttcaa ggttgcaatc tttcttaaca gacaccattg
     4021 catgtaacca attcttcttg acggcaaagt attcatctaa cttatgcatg aaccatgttt
     4081 tgccatatcc tgctggagca tcaacaagaa aatattgccc acccgatgag gctgttgctg
     4141 taacagcttc aatttcatcg actcgattta cgaacattac cgaggaaaaa ttagaccctg
     4201 ccgtgattgt gttctctttg gaaaattcaa tctgcccatc aaactcgaat aatggtcctt
     4261 tcgagacgtg ttcaggaaag gctcgtttca attcatttgc acgtactttc agagcattgg
     4321 taagtttttc atacccctta gccgtaaata aatcgacagc ttgtttctct ttcatcggtg
     4381 ctggcaatga acatctttcg aactttgcag ggattaagta aatggttcca tctggctttt
     4441 ccttgtctgc ctccaatgcc aaagtaattt cctcgtgtat aaacccctct ttagcaattg
     4501 ttttcttcag caaacaaatt atgacaatgt ctgagctttt tatctttttt cggatttcta
     4561 ctttccaatt ttgtccccca ataggtattt cttcgtctaa gatacagtca attccgtcgg
     4621 cgcacagtcg gcgatgtaat aaacgcacat gctcctgagc ttcctttccg gagcataaaa
     4681 agacacgcaa ctgctttttt gtcataatcc ctcaccatga cgagcgtcct tttccacagc
     4741 agaaaaggtt atatatgctt aatcattata ggcaatgcaa tattcaaagt caatcaaaat
     4801 acaggtgaca ttgctccttg ttataaagaa atcacgcttg gcttcagccc taactcatca
     4861 atcagcccgt tcaattcctc gtccgttagc ctgcgtccct tgtccgcata ggcagtgagc
     4921 atggcttcag tggtgtttgt gccaaaggag cgaccgtcgt aacgtggggt agtggtgatg
     4981 acagttttga cgcctctttc tttgaggagg gcgatatttt cctcggttgt tgtatttgtc
     5041 acgacagttt tacctgccaa gcttttgggc atgtatttgc gcataaagag gaaatcaccg
     5101 gcgattaagt cagattcttc aaagtatttg acatacttcg gttcttgttc agcgccatca
     5161 ctgccataaa agatcatgga catgggaaaa tagctgacca tcggcaacat gactcgtgct
     5221 actcgcttaa aagcagggat tccgtgtaat ggaatgggca cacccaatgc caccatcaaa
     5281 tcaccagaga cgatctggtc agagacttca gcaacagctt ctgccaaccc catacggtct
     5341 actgccacag ggatgaaagc ttgcttgaag cgcacttcgc ctaattcggc tttggctaat
     5401 tggaagactc ggcgttcaag ggtgtgtttg agtccgcgcc cgtcacagag tggagtctgt
     5461 ttgacgccag agacaagttt gagcgcagca tgaagcgggt attctcttgc atccaagcgt
     5521 aggtaaaggt ccacaccacc aacaccaaag gcatccactt ttccatccaa ttcgagatag
     5581 agtctgcggg ctttttcaat atcgccatcg gtgccaattc gctcgacttg gatttccaca
     5641 ccgttgaggt tgacgatgac ttttttatcg cgctttgaac ttccaaggct gatacccact
     5701 gcacgtttca tggcacactc tttctttttt tattcactca caaacggatt atttttcttt
     5761 tcatgcccta ctgtggtttt cggaccatga cccgataata agacagtttc atcgggcatg
     5821 gtgtagattt gttcgcggat acttttctca agcgctgaaa aactgctccc cggaagatcg
     5881 gtgcgaccga cactttcgtt gaagattaaa tcgccgcaga agcagatgct ttccttcgct
     5941 acatacaata cacaatgtcc gcgggtgtga ccgggtgtga agcggacttg gaagtcgttc
     6001 gagccgagtt tcatggtcat gccgtggatc aggtcaatgg tcggttcggg accggggtct
     6061 atatccaaac caaatttttt accgccgccc ccttcgcgcc acagtacgtg gtcatcgggg
     6121 tgaagcgcca catgtgggag cggattcatt gcatcggcaa tagctgctgc cccgccaata
     6181 tgatcgaagt gggcatgtgt gtaccaaaga tgcccaatac gccatccgcg tttttgtgct
     6241 tcggcgagga tcaaatgtcc atcccaagag gggtctatga ccacggcttc tttggtttcg
     6301 gggtctgcta caagataggc atttgtagat gcgggaccaa gagtgaattg aacgatctca
     6361 agcatgggat tcctttcgag gtagaaatac agagcttaga agaatagaca agaccaataa
     6421 cccaatggag atggggtaaa cgatgaaagg gtcaaattcg aataaaaagc ctgcaattgg
     6481 tggtgtaatg attgtgatga tcgcgctgac agtttccatc acgccataag tgacacccat
     6541 ctgtgattca tggacaagct cgcgcgcttg tgccaatgcc atggggcgcc ccgctcgaaa
     6601 accacccaac aagaaatacc ctaacgcaaa gaacggaaga gaagttccat accagatcag
     6661 aaccgcaaat agaataacca acgactgtgc tgctaaaaat ccgtaccgcg gattcacacg
     6721 gctgaacgtg atcgccatca atgaatttcc gagtgcgcca atggtaaaaa taagacccgt
     6781 ctggctgagt gaaagtccgc gcacgccttc tagaaaatta ggcgtaagtg gttgtgcaag
     6841 gtacatggag aaaatcgcaa atgccagaac gaacatcaag gtgacaaatt ttttattgct
     6901 gcccaaacct agcggaggag cagatgggtc atgatggtct atgggctggc ttccaataaa
     6961 atacacgaaa atgttggaaa atacaaacac aacggcagcc gcataaaagc tcatacgcat
     7021 tccatagtgt tcgccgatcc aaccactggt tacgggtcct agaaccatgc ccattccgaa
     7081 tgtagctgtg gtgagtgaaa gcacggttgc aacaggcaac tgtccgcgtg cagcagttac
     7141 atagctgctt aatggtgatg acacaaaagc tgtcagacca tatgtaaata aaccgattaa
     7201 gaaaaagggc agcgtctttg cgcttcccat tgtaagagtt gaaagtagac ccaacagcca
     7261 agcaacaata agcaaaggtt ttctacccag gcggtctgcc aaccttccag ccgggatgtg
     7321 tgtgatcgcc atgaaaaagc cgaacccgcc aagaatgagc ccaatttcgt atttacttaa
     7381 caaaaattgc acatccaaat agatcggcac aaaattgaag aacatgcctt cgccaaatcc
     7441 ccataacagc aatgccgcag caatgaaaga aagatt
//
LOCUS       DAOQJE010000041         7458 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_5443_length_7458_cov_7.121707, whole genome shotgun sequence.
ACCESSION   DAOQJE010000041 DAOQJE010000000
VERSION     DAOQJE010000041.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 7458)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7458)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7458
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5443_length_7458_cov_7.121707"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..3991)
                     /locus_tag="PLT08_01920"
     CDS             complement(<1..3991)
                     /locus_tag="PLT08_01920"
                     /inference="COORDINATES: protein motif:HMM:NF021570.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2298 domain-containing protein"
                     /protein_id="HNE03247.1"
                     /translation="MDNKKHFWLYDILFILVFLVAGYLRIVGYDWGEGQHQHPDELFL
                     TGVLDNLRAHTCNDPAIPIDACPEENQRWMTVSEYFDSTTSTLNPYNRGFGFFVYGNL
                     PMTVTRVLMEAIGKDDIGSSKFFARQMSALADLLTIFLLYLIVSRLYGQKIGLLASVF
                     SSLAVMQIQQSHFFTSDLFVNLFMFLALLFAVGLVEWQGRTDDQPEELETVIKVEDST
                     ETAEPVKSSISTQIQILKHPLFYLTVAFGFALGMAMASKINAAAMALLLPFALAIRWL
                     VYDRKQKLSNEYWMQVAILLMVGGFATLVSFRIFQPYAFDGIGLNAQWVKNISEQRAQ
                     ASGAADLPWNLQWIRRTHLYSFENLTIWGLGLPLGILAWIGFLWMGWRILKGEYKHLL
                     LWGWTAFYFAWQSMQFNPTMRYQLPIYPLLAMMAAWVLFEIAGANTPVVNRSNLRKVS
                     ATVLGLTVLTLTAVWAFAFQSIYLRAEPRIAASRWMFENAPAGINVLIQTKEDTFNQP
                     LPIPSGTLITPENPYSVTFSPQREGVFNEVMLGHVANVSSTPTTVDLNIYAYEQPTVS
                     LAHAATVIDPASVIAGNTLPLNLMPEQAFVLAPSQKYVLQLETNGNGIYVSESFSILL
                     QTSEGDFIQTVPISPGLFIAPGNPYQMVFTPLKEGSFNGLKFGYALDTVKSPSNVSVA
                     VYSASQPDTKLAQASAVLSPTSTDNFRGDSLTLQFDRPIPLITDQQYVLKVETDGSGI
                     FVSGSAIVNETDYDFPLPFRVDGYDPYGGLYSNDDLNLQVYWADDANKIDRFVNILSK
                     ADYIAIPTNHQYAQITRVPERYPLTTFYYRELLGCPEGEDIIKCYRVAEPGMYEGKLG
                     FELEAVFTTYPTLGPIVINDQAAEEAFTFYDHPKVLVFKKTDDFNAENLRAILSTVDV
                     SKAVQLTPKDFASFKSLMLPEDRLASQRAGGTWSDLFSYSWLQNKIPALGLVLWYVFI
                     LVLGLFAYPIARLALPGLRQYAYALGRIVGLALMAWLAWMGGSIGVPYTRLSIGVAFA
                     LIVVAGIGLWMKRKEEFKADWDQHRQFFVLVEIVFLTFFLIDLLIRIGNPDMWHPSKG
                     GERPMDFSYFNAVLKSTSFPPYDPWYAGGYINYYYYGFVLAGTPVKLLGIVPSIAYNF
                     ILPTWFAMVALGAFTVGYGAVASSKADGEADEPSMFNLQLLAGLAGALLTVFLGNLGT
                     IQLIFNAMQRVAAPGGTVPTETGFIQHWVWAFQGIWKMITEKAMLPIGRGDWYWFPSR
                     VIPTGPGNEITEFPLFTFLYSDLHAHMLVMPLTLFIIAWALAFVRSRAQLTRGEWIAS
                     FGIGALMIGA"
     gene            complement(4019..6175)
                     /locus_tag="PLT08_01925"
     CDS             complement(4019..6175)
                     /locus_tag="PLT08_01925"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HNE03248.1"
                     /translation="MESNEHTSPQEELTVLDLYKSVTKDWSSFFKFIRSLGDVRRREE
                     FNQALAQETAQAVMIEQTAEGLQPKRAAVDFPFRAVLALSIAFGAQFLIEPPNRFGML
                     SFALYLAAFGVAIWSYRSDEWRLPALPVAWQRFDPLSFKFIPLVIATVLAVLAFVDFG
                     SGLFSVRNTLIWFLAVAFICYSLWLQVAKTPETDSVDNAPNVRRKKWVQPVLFLIVFG
                     IALFYRLYSLNTVPNEPFSDHAEKILDVYDISQGYPKVFFERNTGREAIQMYWTLLVL
                     TVFNTGFSYLSLKLGTILLGILTLPYVYLLGKEYGSERLALFAMLLFGIAYWPNVISR
                     IGLRFPLYPLFVAPTLYYLIRGFRMQNRNDFILAGIFLGLGLHGYSPFRMVPIFVVVM
                     FGIYFLHAKTKDERRQAIWWLLIVVVISMFVFLPLLRYWLEYPQNFGYRAFTRLSDTE
                     TALPGPAWQIFLSNLYKGLLMFNWDDGEIWVHSVVHRPALDLVSAVFFAVGAIFLIVR
                     YFRAKDWRDLTLLSSIPVLLMPSVLSLAFPGENPSLNRTGGAAIPVILIVAMALDGFL
                     SSFRSEKRRLFIGYGLTFLLLGYSMYLNYDLVFNQFASSYRASSWNTSELGKQLSDFR
                     AKYGQLDTAWIVPFPYWVDTRLPGVWAGVPDRDFAMWQDQLPSSLSYPAPKLFMFWHD
                     DLETEKVLKELYPNGKLTRYTSAVEGKDFYIFLVEP"
     gene            complement(6352..7122)
                     /locus_tag="PLT08_01930"
     CDS             complement(6352..7122)
                     /locus_tag="PLT08_01930"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNE03249.1"
                     /translation="MNEQRSKDFVFLQVSSLPYFRALLRAVESEFYQDLPLPSPVYDV
                     GCGDGHFASLTFDHKLDVGLDPWRFSMKEAKRYNAYKSLVEADGAQTPFPSNHFASGL
                     SNSVLEHIPHIDAVLKETARVLKPGAPFYFCVPNANHFEELSLRKIFGKPYEMWFRKI
                     QRVQHSDHPDVWEERLERAGFKLERWWHYFSPSALRVLEWGHYFGLPSLVAKALTGNW
                     IISPTKWNLFLTEKYIRKYASTEPIENGTYTFYIARKK"
     gene            complement(7119..>7458)
                     /locus_tag="PLT08_01935"
     CDS             complement(7119..>7458)
                     /locus_tag="PLT08_01935"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNE03250.1"
                     /translation="VQMAAYYPNLDVITVPSLNSTEAFGLVQIEAMMNGVPSVPSALP
                     GVRQPVKMHGMGVVSEIGNSESLATAILEVLDHKEKYRGDLSAIKAAYNPDTVAQEYE
                     KLFEKLMRKK"
BASE COUNT         2312 a         1756 c         1600 g         1790 t
ORIGIN      
        1 gtgcgccgat catcaaagcg ccaataccaa aggaagcaat ccattcaccg cgagtcaatt
       61 gcgcacgaga gcgcacaaat gccaatgccc atgcaatgat gaacaaggtc aacggcatca
      121 ccagcatatg agcatgtaaa tcactgtata agaaagtgaa cagcggaaat tctgtaatct
      181 cgttaccggg tccggttggg attacgcggc ttgggaacca ataccaatct ccgcgcccga
      241 tgggcaacat cgctttttcg gtgatcattt tccaaatgcc ttggaatgcc cacacccaat
      301 gctggataaa acctgtctca gtaggaaccg tcccacctgg ggctgcaaca cgttgcatgg
      361 cattgaagat cagttggatc gtccctaaat ttccaaggaa gaccgtgagc aatgcgcctg
      421 ccagcccagc caagagttgc aagttgaaca tagatggttc gtctgcttcc ccatccgctt
      481 tgctgcttgc aacagcgccg tatccaaccg tgaaagcacc caacgccacc atggcaaacc
      541 aagtaggcaa aataaagtta tatgcaatcg acggcacaat gccaagcaat ttcaccggcg
      601 tgcccgccaa cacaaaccca taatagtaat aattgatata gccaccggca taccacgggt
      661 catagggtgg gaagctagtg ctcttcaaaa ccgcattgaa atacgagaaa tccattgggc
      721 gttcgccgcc tttggatgga tgccacatat ctgggttgcc aatgcgaatg agcaaatcga
      781 tcaggaagaa ggttaagaag acgatctcaa ctaagacgaa gaactgacga tgttggtccc
      841 aatctgcttt gaactcttct ttgcgcttca tccataagcc gatgcctgcc actacgatca
      901 aagcaaatgc cacgccgatg ctgagtcggg tatagggcac accaatcgag ccgcccatcc
      961 acgccagcca tgccataagt gccaagccaa cgatcctgcc taaagcatag gcatactgcc
     1021 gtaaccccgg caacgccaaa cgcgcaatcg ggtaagcgaa caatcccaac acaaggatga
     1081 atacatacca caataccaat cccaaggcag ggatcttatt ctgcaaccaa ctgtagctaa
     1141 acaagtcaga ccatgttcca cccgcacgtt gacttgctaa acgatcttca ggcagcatca
     1201 ggctcttgaa ggaagcaaag tctttgggag taagttgaac cgctttgctc acatcaactg
     1261 tgcttaagat ggctcgcaga ttctctgcgt tgaaatcatc agtcttcttg aagactaaca
     1321 ccttaggatg gtcataaaaa gtaaatgctt cttcagctgc ttgatcatta atgacgatgg
     1381 gtcccaaggt tggatatgta gtgaaaactg cttcaagttc aaaaccaagc ttgccttcat
     1441 acataccagg ttcagcaact cgataacact taataatatc ttccccttca gggcatccta
     1501 aaagttcgcg ataatagaag gtcgttaatg gatagcgttc tggcacacga gtgatctgcg
     1561 catattggtg attggttgga atggcaatgt aatccgcttt ggataagatg tttacaaagc
     1621 gatcaatttt attggcatca tccgcccagt acacttgcaa gttaaggtca tcgttgctgt
     1681 ataacccgcc ataggggtca tatccatcca ctcgaaatgg caatggaaag tcataatctg
     1741 tttcattaac aatcgcagaa ccagaaacaa atattccact cccatccgtc tcaactttca
     1801 gaacatattg ttggtcagta atcaatggga tcggacgatc aaactgaagc gtgagagaat
     1861 ctccgcgaaa gttatcagta gaagtggggc tcaaaactgc cgaagcctga gccagttttg
     1921 tgtctggttg tgaggcggag taaacggcta cacttacatt tgatggtgac tttactgtat
     1981 caagggcgta tccgaacttc aatccattaa aagaaccttc ttttaatggt gtaaagacca
     2041 tctgataagg atttcctggg gcgatgaata agcccggaga gatggggacg gtctgaataa
     2101 aatcgccttc actcgtctgc aacagaatac tgaaagactc tgaaacatag atgccgttgc
     2161 catttgtttc aagttgcaat acatatttct ggctcggcgc caacacaaaa gcctgttcgg
     2221 gcatcaagtt caaaggaaga gtattccccg ctatcacaga tgctggatca ataaccgtgg
     2281 cggcatgtgc caagcttaca gttggctgtt cataggcata aatattaaga tcaaccgttg
     2341 ttggtgtgct cgaaacattt gcaacatgcc ccaacatcac ctcattgaag acgccctctc
     2401 tttgcggaga gaaggtcaca gaatatggat tctctggagt gatcaatgta ccggatggaa
     2461 tcggtaaagg ctgattaaag gtatcttctt tcgtttgaat aagaacattg ataccagcag
     2521 gcgcattttc aaacatccaa cgggatgctg caatacgcgg ttcagctcgc aaataaatac
     2581 tttggaacgc aaatgcccac acagccgtaa gcgtaagaac tgtcaagcca agaacggttg
     2641 cagaaacttt gcgcaaattt gagcgattta ctactggtgt gttcgcccct gcgatctcaa
     2701 aaagaaccca tgctgccatc attgccaata acggatagat cggcaattga taccgcatag
     2761 tagggttgaa ctgcatagat tgccatgcaa agtaaaaagc agtccacccc catagcaaaa
     2821 ggtgtttgta ttcgcctttt agaatacgcc agcccatcca taaaaaacct atccaagcaa
     2881 ggattcccag agggagccca agcccccaaa tggtaaggtt ttcgaatgaa tacaaatgag
     2941 tacggcgaat ccattgcaaa ttccacggca ggtctgctgc gccagatgct tgagcgcgtt
     3001 gctcggagat attcttcacc cattgtgcat tcagccctat accgtcgaaa gcatagggtt
     3061 gaaaaatacg aaaagataca agggtagcga atccgccaac catcaaaaga atcgctacct
     3121 gcatccaata ttcattgcta agcttctgct ttcggtcata aacaagccag cgaatagcta
     3181 acgcaaaagg taagagcaat gccatggcag cagcattgat tttggacgcc attgccattc
     3241 ccagagcaaa accaaacgca accgtcaagt aaaaaagtgg atgtttcaga atctgaatct
     3301 gagtagaaat gctgctttta acaggttcag ccgtttcagt gctgtcttca accttaataa
     3361 ccgtttctaa ctcttctggc tgatcatctg ttcgtccctg ccactcgaca agtcccactg
     3421 cgaagagcag ggcaaggaac ataaataggt ttacaaaaag atctgaagta aaaaagtgag
     3481 attgctggat ctgcatcact gctagtgaag aaaatacaga agcaagcaac cctattttct
     3541 gtccatacaa acgcgaaaca ataagatata gcaggaaaat cgtcaacagg tctgccagag
     3601 cagacatctg ccgcgcaaag aactttgagg aaccaatatc atctttcccg attgcttcca
     3661 tcaaaacgcg agtcactgtc attggcaaat tgccataaac aaaaaaacca aacccgcgat
     3721 tatacgggtt caacgtagac gtagtgctat cgaagtattc actcacagtc atccagcgtt
     3781 gattttcttc cgggcaagca tcgattggaa tagccgggtc gttacatgtg tgcgcacgca
     3841 gattatccaa cacacccgtc aagaaaagct catcaggatg ttggtgctgc ccttctcccc
     3901 agtcataccc aacaatgcgc aaatatcccg ccactaaaaa gacgagaata aacagaatgt
     3961 catacaacca aaaatgtttt ttattatcca ttgatcactg taaaagggct aaccattatt
     4021 atggttccac caaaaagata tagaaatcct taccttcaac cgctgaagta tagcgggtta
     4081 attttccatt tggatagagt tctttaagaa ccttctcagt ttcaaggtca tcatgccaga
     4141 acatgaacaa tttcggagca gggtaactca agctactagg caattggtcc tgccacatgg
     4201 caaaatcacg gtctggcacg cctgcccaaa ctcctggcaa gcgagtatcg acccaatagg
     4261 gaaatgggac aatccatgct gtatccaatt gcccatattt agcacgaaag tcacttaact
     4321 gtttgccaag ttcagatgta ttccatgatg aagcgcgata ggaactcgca aattgattaa
     4381 agaccaaatc ataatttaga tacatggaat agcccaagag gaggaatgtc agcccgtatc
     4441 ctataaataa acgcctcttt tcggacctga aacttgagag aaagccatca agcgccatcg
     4501 ctacgataag aatcaccggg atagcagcac caccggttcg attcaatgaa gggttttcac
     4561 cggggaatgc cagcgaaagt acagatggca tgagcaggac agggattgaa ctcagtaagg
     4621 ttaagtcacg ccaatcttta gcgcggaagt aacgcacaat taagaagatt gcgcccactg
     4681 cgaaaaacac tgcggaaacc aggtctaatg ccggtcggtg caccacagag tgaacccata
     4741 tttccccgtc atcccagttg aacatcaata agcccttata caggtttgaa aggaaaatct
     4801 gccaagctgg tccgggtaat gctgtttcgg tatcgcttaa acgagtaaat gcccggtaac
     4861 caaaattctg tgggtattca agccaataac gcaataatgg caggaagaca aacattgaaa
     4921 tgacaaccac aatcaatagc caccaaatcg cctgacgtct ttcgtctttt gtctttgcat
     4981 gtagaaaata aataccaaac atgaccacca caaagatggg aaccatacgg aaaggactat
     5041 atccgtgcaa gcctagccca agaaagatac ctgcaagaat aaaatcattt cggttctgca
     5101 ttctaaagcc acggattaga taatatagcg ttggcgcaac aaacaaaggg tatagaggga
     5161 agcgcaaccc gatccgcgaa attacatttg gccagtatgc aattccaaaa agcaacattg
     5221 caaacaaggc cagccgttca cttccatact ccttaccgag caggtaaacg tatggcaacg
     5281 tcagaatacc aaggagaatg gtaccaagct taagactgag gtaggaaaaa cctgtattga
     5341 aaacagtgag cacaagcaat gtccagtaca tttgaatcgc ttcacgcccg gtattgcgtt
     5401 caaaaaagac ttttggatat ccttgtgaaa tatcatatac atcgagaatt ttttcagcgt
     5461 gatcactaaa tggttcattt ggaaccgtat taagtgaata taggcgataa aacagtgcaa
     5521 tcccgaaaac aatcaagaac aaaacaggct gcacccactt ttttcgcctg acgtttggtg
     5581 cattatctac tgagtccgtt tcaggcgttt ttgcaacctg aagccataaa ctataacaaa
     5641 tgaacgctac agccaagaac catataaggg tattgcgtac agaaaaaagc ccgcttccaa
     5701 aatcaacaaa ggctaaaacg gctaaaaccg ttgcgatcac caaagggata aacttgaaag
     5761 acaatggatc aaatcgttgc catgcaacag gtaaagcagg caaacgccat tcatcagacc
     5821 ggtaagacca aatggcgacc ccaaacgcag caagatacaa tgcaaaggat agcattccga
     5881 aacgattggg aggctcaata aggaattgag caccaaatgc aatgcttaga gcaagaacag
     5941 ctctaaatgg gaagtcaaca gcagcccttt tgggttgcaa gccttctgca gtttgctcaa
     6001 tcatcactgc ttgcgcagtt tcttgcgcaa gagcttggtt aaattcttct ctacgccgta
     6061 catcccctaa cgagcggata aacttaaaaa acgaggacca atcctttgtg accgatttat
     6121 aaaggtccaa aacagtcagt tcttcttgcg gggatgtgtg ctcatttgat tccatatact
     6181 ctctcaacct aaaatgtaaa ttgttgcaga acagggtaat tctatactaa ttttgggcgt
     6241 gtttaagtgc actatattaa gaatttatgg caaaaatagc gaaaagcgcc ctttcaaggc
     6301 gcttttagac agaaaaggct gaaaataaaa tccgtatttt cagcctttta attatttttt
     6361 cctagcgata tagaatgtat atgttccgtt ctcaatcggt tctgtggatg catacttacg
     6421 aatatatttt tcggtcaaaa agagattcca ttttgtaggt gaaatgatcc aattacctgt
     6481 taatgccttt gccactagag agggcaatcc gaagtaatgc ccccattcta agactcgtag
     6541 agcagatggc gaaaaataat gccaccaacg ctccaacttg aagcctgcac gttcgaggcg
     6601 ttcttcccat acatccgggt ggtctgaatg ttggacccgt tgaattttac ggaaccacat
     6661 ttcatatggt ttgccaaata tttttcgaag cgaaagctct tcgaagtgat tggcgttcgg
     6721 cacacaaaaa taaaaagggg ctcctggctt aagcacacgt gctgtttctt ttagaactgc
     6781 atcaatgtgc ggaatgtgct ccagaacgga gttgcttaat ccgctggcaa aatgattgga
     6841 agggaacggt gtttgtgcgc catcggcttc gaccaaggat ttataggcat tgtatcgttt
     6901 cgcttctttc atggagaaac gccacgggtc aagcccgaca tccagcttgt ggtcaaatgt
     6961 tagtgaagcg aagtgaccat caccacaacc cacatcataa accggagagg gtaatggcag
     7021 gtcttgataa aactcagact caactgcccg caacagggcg cgaaaatagg gtaatgaact
     7081 cacctgcaaa aaaacaaaat ctttggatcg ttgttcgttc actttttcct cattaacttt
     7141 tcaaacaatt tttcatattc ctgtgcaacc gtatcagggt tatatgcggc tttaatggct
     7201 gaaaggtcac cgcgatattt ttctttgtga tcaaggactt caagaatggc agttgccagg
     7261 ctttctgaat tgccaatctc agagacgaca cccatgccat gcatcttcac aggttgacgt
     7321 acccctggca aagcagaggg cacactcggc acaccgttca tcatcgcttc gatctggact
     7381 aaaccaaatg cttcggtcga attgagagat ggcacggtaa taacatccaa gttcgggtag
     7441 taagctgcca tctgaaca
//
LOCUS       DAOQJE010000042         7424 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_5484_length_7424_cov_7.685982, whole genome shotgun sequence.
ACCESSION   DAOQJE010000042 DAOQJE010000000
VERSION     DAOQJE010000042.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 7424)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7424)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7424
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5484_length_7424_cov_7.685982"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..627)
                     /gene="truA"
                     /locus_tag="PLT08_01940"
     CDS             complement(<1..627)
                     /gene="truA"
                     /locus_tag="PLT08_01940"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HNE03251.1"
                     /translation="MLVSVMARYQVILAYDGAGFTGSQRQANSRTVQGELEKALHQIG
                     WTGKSVMMAGRTDTGVHAAGQVAAFDFNEWSHPAENLLQALNAKLPADLAVRKISSVH
                     AEFHPRFDALSRAYRYKLFCEPIRDPLREKVAWRLWPKPDSDALNQTAEIFLGTHDFA
                     AFGSPTTEKGTTTRTVTNSEWRRKPNGEWQFEVRADAFLYRMVRRLVFV"
     gene            complement(654..1031)
                     /gene="rplQ"
                     /locus_tag="PLT08_01945"
     CDS             complement(654..1031)
                     /gene="rplQ"
                     /locus_tag="PLT08_01945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005762 - mitochondrial large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HNE03252.1"
                     /translation="MRHSVAGYKLGRTKSSRIALRRNLIKQLFTHERIQTTQAKAAAI
                     RGDAERLITLAKNSADATADQKVHARRVAVSKLGDNQIIKRLFDEIAPRFASRNGGYT
                     RVIKLGPRLGDAAEMVVLELVEE"
     gene            complement(1035..2003)
                     /locus_tag="PLT08_01950"
     CDS             complement(1035..2003)
                     /locus_tag="PLT08_01950"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HNE03253.1"
                     /translation="MTGLITNMVMPKIEREAEARNYGKFVISPLEGGYGVTLGNALRR
                     VLLSSLEGTAITSVRFADVLHEFSDIPGVREDVIQVMLQIKQIRIKMDGVDSARMHLE
                     VRGEGTVTAADIITPAEIEIVNPDTYLFTVDNPKTKLDVEFVVERGRGYSPSNERSGH
                     MPIGELPVDAIFSPVKRVNWEVASARVGQSTNYDKLILEIWTDGTVSPERALSSSARI
                     LIDHLRYIAGVNEEMLTVAVERESSGSRLSSELAETPVEALDLSVRVFNSLKRTGITT
                     VGDVLELLEKGESAVMSIRNFGEKSLDELRDKMREKGFLKDQSSEN"
     gene            complement(2075..2731)
                     /gene="rpsD"
                     /locus_tag="PLT08_01955"
     CDS             complement(2075..2731)
                     /gene="rpsD"
                     /locus_tag="PLT08_01955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HNE03254.1"
                     /translation="MAKSLSPVCKLCRREGEKLYLKGERCFTPKCAFDRRSFAPGQHG
                     KTSGRGGNPGSTGRASDFARQLRAKQKARRVYGVLERQFRRYYDTAITRRGLTGLNLL
                     QILESRLDNVVFRLGFASSRAQARLLVTHGHFTVNGRRTDVPSMLLSNGDVIAVREGS
                     GKLSYFKDLNAYAEKRNTPAWLSRDLKAMSGSVARMPERAEIDGSLDEQLIVEYYSRR
                     "
     gene            complement(2748..3152)
                     /gene="rpsK"
                     /locus_tag="PLT08_01960"
     CDS             complement(2748..3152)
                     /gene="rpsK"
                     /locus_tag="PLT08_01960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004074888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HNE03255.1"
                     /translation="MAQSVRSTRRAGGAKKGKRSLSRGQVHIFASFNNTIVTVTDTEG
                     NTISWGSAGSAGFKGSRKSTPFAARLAAEQAIKSAQQQGLQEVELYVKGPGPGRENAI
                     RAVQAMGLKVLLIADITPVPHNGCRPPKRRRV"
     gene            complement(3177..3563)
                     /gene="rpsM"
                     /locus_tag="PLT08_01965"
     CDS             complement(3177..3563)
                     /gene="rpsM"
                     /locus_tag="PLT08_01965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HNE03256.1"
                     /translation="MARIEGVDLPRNKRTEVGLTYLFGIGPTRAKKILADTKVNPDTR
                     IKDLTEAEVSAIREYIAKHYKVEGDLRREVQMNIKRLIEIGSYRGLRHRRNLPVNGQR
                     TKTNARTRKGTKKTVAGRGRRRGAKK"
     gene            complement(3571..3684)
                     /gene="rpmJ"
                     /locus_tag="PLT08_01970"
     CDS             complement(3571..3684)
                     /gene="rpmJ"
                     /locus_tag="PLT08_01970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003720928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HNE03257.1"
                     /translation="MKVSPSIRKRCAKCRIVRRKGIVYIICENPKHKQRQG"
     gene            complement(3784..4554)
                     /gene="map"
                     /locus_tag="PLT08_01975"
     CDS             complement(3784..4554)
                     /gene="map"
                     /locus_tag="PLT08_01975"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HNE03258.1"
                     /translation="MFHERQINLKTPAELKIMREAGRINASVHAKLRELLQPGVTTAD
                     LNAAAEEVLKSHGCVSPFKGYGHPPFPASITVSINDEMVHGIPNSKRKLKEGDVVSLD
                     CGTVYQGYVADSAFSAGVGQISQEAQTLLEVTEAALQAGIAKMRKGNRTGDVSAAIQK
                     YVESHGLHVTREYTGHGVGKSMHEGPQVPNYGMAGTGLVLKPGMTIALEPMVLVGTYE
                     TRVLPDKWTVVSADGSLTAHFEHTVAVTEGEPQILTVL"
     gene            complement(4606..5256)
                     /locus_tag="PLT08_01980"
     CDS             complement(4606..5256)
                     /locus_tag="PLT08_01980"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016776 - phosphotransferase activity,
                     phosphate group as acceptor [Evidence IEA];
                     GO_function: GO:0019205 - nucleobase-containing compound
                     kinase activity [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HNE03259.1"
                     /translation="MPTYIVLLGPPGVGKGTQARILSEETGLVHVSSGDLFRENLKNQ
                     TELGKLAKSFMDKGELVPDDVTINMIKDRISRPDCKAGAVLDGFPRTPAQADALEVML
                     KSFEGAVNAVPYITAAEHILVERASGRWTCRANGHIYHEKFNPPKQARVCDIDGSEVY
                     QRDDDKVETVTKRIRVYLEQTMPLVEYYRKAGKLIEIDGTQAVEEVTKVLLSALKK"
     gene            complement(5269..>6069)
                     /locus_tag="PLT08_01985"
     CDS             complement(5269..>6069)
                     /locus_tag="PLT08_01985"
                     /inference="COORDINATES: protein motif:HMM:NF012564.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SecY family transport protein"
                     /protein_id="HNE03260.1"
                     /translation="GQGLSLVIFAGIVARIPANFASLLADETNRWIMLTLTVIIIVLT
                     VLAIVYVQQGRRNVPVMYPGRRVGNRMSMPVKGTMPLMVNLSGMIPLIFASAILQFPA
                     IIASYFTQSENQAVANFFVGIQNFFGATGTSNWGYWTIYFFMVVVFTFFYTTVLFDQQ
                     NYGENLKKVGASVPGVLTGAPTQKYLSTVQSRITFVGALFLGIVAIMPFLLGLLLAAF
                     NISASNSTGIFLITSSGLLIVVGVVRDTFQNIDAELKLHGYQDSLLVR"
     assembly_gap    6070..6169
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<6170..6444)
                     /locus_tag="PLT08_01990"
     CDS             complement(<6170..6444)
                     /locus_tag="PLT08_01990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE03261.1"
                     /translation="MKTTFSGWHYLWKSEDIRRKLIVTFVILVIYRLAANIPAPGVDH
                     DILAAFRSSTSGSGGFLGFLDLLSGGTVSNFSLLSMGVYPYITAQII"
     gene            complement(6461..6916)
                     /gene="rplO"
                     /locus_tag="PLT08_01995"
     CDS             complement(6461..6916)
                     /gene="rplO"
                     /locus_tag="PLT08_01995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HNE03262.1"
                     /translation="MKLHDLVPNPGSKKNRKRVGRGISAGQGKTAGRGTKGAGARSGE
                     GGHLYRQGGNLPFYRRLPFMRGEGFTPPNQVEFNEVNLDQLSEAFKANAEVNPESLAE
                     AHLLRDTRNPVVILGRGEMSVALKVRVHRVSAGAKAKIEKAGGSVELIA"
     gene            complement(6924..7118)
                     /gene="rpmD"
                     /locus_tag="PLT08_02000"
     CDS             complement(6924..7118)
                     /gene="rpmD"
                     /locus_tag="PLT08_02000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_041604457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HNE03263.1"
                     /translation="MPKNTSGKSIRVTLVRSAIGYTKDQKATVKALGLRRMHQTVEHK
                     DTPALRGMLNKIIHLIKIEE"
     gene            complement(7111..>7424)
                     /locus_tag="PLT08_02005"
     CDS             complement(7111..>7424)
                     /locus_tag="PLT08_02005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HNE03264.1"
                     /translation="TVNIAGTTIPHESLGKVVGAKVFLKPASAGTGVIAAGGVRAVLE
                     VAGVRDILTKSMGSANVLNVVMATFDALDGLRSPANEAARRGKKAEELLPYWERRKQH
                     A"
BASE COUNT         1719 a         1930 c         1924 g         1751 t
ORIGIN      
        1 aacgaacacc agtctgcgca ccattcgata taggaacgca tctgcccgga cttcaaactg
       61 ccactcacca tttggctttc gtctccactc ggagtttgtc accgtgcggg tcgtcgtccc
      121 cttttcagtg gtcggagacc cgaaggcggc aaaatcatgt gtgccgagaa agatttcagc
      181 agtttggttg agagcatcac tatctggctt gggccacaag cgccatgcta ccttttcgcg
      241 taacgggtca cggataggtt cacaaaacaa cttgtaccgg taagctcgag agagcgcatc
      301 gaaccgcggg tggaattccg catggacaga acttattttt cgtaccgcca ggtctgctgg
      361 cagtttcgca ttgagcgcct gcaagaggtt ttctgccggg tgcgaccatt cgttgaaatc
      421 gaatgccgcc acctgccctg ctgcatggac gcccgtgtcg gttctgcccg ccatcatgac
      481 ggattttcca gtccagccga tttggtgaag ggctttttca agctcgccct gcaccgtccg
      541 ggagttcgcc tgtcgctggc tgccggtaaa cccggcgcca tcgtaggcaa ggatcacttg
      601 gtaacgtgcc attacactaa ccagcaacca gtgttggtca ctgattactg ttcttactct
      661 tccacaagtt cgaggacaac catctcagcg gcatcgccga ggcggggtcc aagtttgatc
      721 acgcgggtgt aaccaccgtt gcgtgaagca aagcgagggg caatctcgtc aaacaagcgc
      781 ttgatgatct gattatcacc gagtttggaa acggcaacac ggcgagcgtg taccttctgg
      841 tctgcggtgg catcggcgct gttcttggcg agtgtaatga ggcgttcagc atcaccgcga
      901 atggcagcag ccttcgcctg ggtggtctga atgcgctcgt gcgtaaagag ctgtttgatc
      961 aggttgcgac gcagagcaat acgcgagctc ttggtgcgtc ccaatttgta acctgctact
     1021 gaatgtcgca tttcttaatt ctccgaagat tgatctttca aaaagccttt ttcgcgcatc
     1081 ttatcgcgaa gttcgtccag gctcttctca ccgaagttgc ggatggacat gacagcggac
     1141 tcaccctttt cgaggagttc gagcacgtca cccacggtgg tgatgccggt gcgcttcaaa
     1201 gagttgaaga cgcgaaccga aaggtcgagg gcttcgaccg gcgtttccgc caattcactg
     1261 ctcaggcggc tgccggaaga ttcgcgttca acagctacgg tcaacatttc ttcgtttaca
     1321 ccagcgatat agcgcaagtg gtcaatgaga atgcgagcgg aggagctgag ggcgcgttcg
     1381 ggtgaaactg tgccgtctgt ccagatttct aaaataagtt tgtcgtaatt cgtgctctgc
     1441 ccaacgcgtg cggaagccac ttcccaattg acgcgcttga ccgggctaaa aatggcatcc
     1501 accggaagtt caccgatggg catatgcccg gagcgttcgt tggagggcga atagccacgt
     1561 ccgcgttcga cgacgaattc aacgtcgagt ttggtcttgg ggttatcgac ggtgaagaga
     1621 tacgtatcgg ggttgacgat ctcgatctcg gcaggggtaa taatgtctgc cgcagtgacc
     1681 gttccttcgc cacgtacttc gaggtgcatg cgagcgctgt ctacaccatc catcttgatg
     1741 cggatctgtt tgatctgcaa catgacctga atgacatctt cgcgcacgcc ggggatgtcg
     1801 ctgaattcat gcaacacatc cgcgaaacgc actgaagtga tcgcggtgcc ttccaaagag
     1861 gaaagcaaaa cgcgacgcag ggcgtttcca agcgttacac cgtagccgcc ttccagcgga
     1921 ctgataacaa atttgccgta gtttcgagct tctgcttcgc gctcgatctt gggcatgacc
     1981 atgttcgtaa tgagacccgt cacggttcac ctttcccttt tgttcaagcc tcgggtcgtg
     2041 aaccagttca aggtccagac ccgaagcgta aggattagcg tcgggagtag tattcgacga
     2101 tcaactgttc gtcgagactt ccgtcaattt cagcgcgttc gggcatgcga gccacagaac
     2161 cgctcatcgc tttcagatca cggctgagcc aagctggggt attgcgcttc tcagcatagg
     2221 cattcaggtc cttgaagtag gacagctttc cagaaccttc gcgcactgcg atcacatctc
     2281 cgttagagag aagcatcgag ggcacatcgg tgcggcgacc gttcacagtg aagtgaccat
     2341 gggtcacgag caagcgagcc tgggcgcggc tggaagcgaa gccaaggcgg aagaccacat
     2401 tatcgaggcg ggattcgagg atctgaagca agttcaaacc ggtcaaacca cggcgagtaa
     2461 tcgctgtgtc gtaatagcgg cggaactggc gttcaagcac gccatacacg cggcgagcct
     2521 tttgcttagc gcgcaactgg cgggcaaagt cggaggcgcg accggtggaa ccggggttgc
     2581 cgccgcgacc agaggtcttg ccatgctgac caggcgcaaa actgcgtcga tcaaaagcgc
     2641 attttggagt aaaacagcgc tcgcccttga ggtagagttt ttctccctca cggcggcaaa
     2701 gtttacaaac cggacttaat gatttagcca taaataaaca acctcactta cacgcgtcga
     2761 cgtttcggcg gacggcagcc gttgtgtggc accggggtaa tatccgcgat caacaacacc
     2821 ttcaagccca tcgcctggac ggcgcgaatt gcattttcgc gtccgggtcc gggaccttta
     2881 acatacaatt ccacttcctg caaaccctgc tgttgagcag atttgatcgc ctgttcagcg
     2941 gcgaggcgtg cagcaaaagg agtgcttttg cgggagcctt tgaagccagc ggaaccagcc
     3001 gatccccagg aaatggtgtt gccttcggtg tcggtaacgg taacgatcgt gttattgaag
     3061 gaagcgaaga tatgaacctg tccgcgagac agggagcgct tccctttttt cgcgccgcct
     3121 gcgcggcgcg tagaacgtac actctgagcc ataatttctt aacctttcgg acaggattac
     3181 ttcttagcgc cacggcgacg accgcgtccg gcaaccgttt tcttggtgcc tttgcgagta
     3241 cgcgcattgg ttttggtgcg ctgaccattc accggcaaat tgcggcgatg gcgtaagccg
     3301 cgatacgaac cgatttcgat caaacgcttg atgttcatct gaacttcacg gcgcaaatcg
     3361 ccttccacct tatagtgctt ggcgatgtat tcgcggatgg cggaaacttc agcttccgtc
     3421 aggtccttga tgcgcgtgtc aggattgact ttggtatctg ccaaaatctt tttggcgcgg
     3481 gtaggtccaa taccaaaaag ataggtcaga cccacttctg tccgcttgtt gcggggaaga
     3541 tcaacacctt caattcttgc catggttcaa ctacccttgt ctctgtttgt gtttaggatt
     3601 ttcgcaaatg atgtaaacaa tacctttgcg ccgaacgata cggcacttgg cacaacgctt
     3661 gcgaatggag ggcgatactt tcatgaaatt tctccttgct ttgcaacttc tctggcaaag
     3721 ccaaacgctt aattatactg gtcgttctca atttcgtcca tgtttttacg gctacccgca
     3781 gggtcataag acagtcagga tttgaggttc tccttcggta acagccaccg tatgttcaaa
     3841 gtgcgctgtc aacgacccat ctgcggaaac taccgtccac ttatccggca ggacgcgagt
     3901 ttcatacgtc ccaacaagaa ccataggctc gagggcaatc gtcatccccg gtttgaggac
     3961 gagaccggtt cctgccatgc cgtaatttgg cacttgagga ccctcatgca tgctcttccc
     4021 gacaccgtgc ccggtgtatt cgcgagtaac atgcaaccca tggctttcta catacttttg
     4081 tatcgccgcc gaaacatctc ctgtacgatt gcctttgcgc atctttgcga tgcccgcttg
     4141 caatgccgct tccgtcactt cgagcagggt ttgagcctct tgtgagatct gccccacccc
     4201 agccgaaaac gcggagtctg ctacatagcc ttgatacacc gtaccgcaat cgagtgaaac
     4261 gacgtcccct tctttcagct tgcgcttcga gttgggaatt ccatgcacca tttcgtcatt
     4321 aatactgact gtgatggatg caggaaacgg cgggtgtcca tagcctttga acggcgatac
     4381 acatccgtgt gacttcagca cttcctcagc agccgcgttg aggtccgctg ttgtcacacc
     4441 tggttgtaaa agttctctta gttttgcatg aacgctggca ttgatgcgcc ctgcttctcg
     4501 catgattttg agttcagcag gagtcttgag gttgatttgg cgttcatgaa acatttttgc
     4561 aaaaccttac tttatttgta cagagtagga tactctggtc tttcgttatt ttttgagagc
     4621 actcagtaat acttttgtta cttcttcaac cgcttgagtg ccgtcgattt cgatcaattt
     4681 gccagcctta cgatagtatt caaccaacgg catagtctgt tcaagataga cacggatacg
     4741 ctttgtaacc gtttctacct tatcatcatc acgttggtag acttcggaac catcgatgtc
     4801 gcagactcga gcctgcttag gcgggttgaa cttctcatga taaatgtgcc catttgcgcg
     4861 gcaagtccaa cgtccactag cacgttctac gaggatgtgt tcggcagctg tgatatatgg
     4921 aactgcattg acagctcctt caaagctttt gagcatcacc tcgagcgcat ctgcttgagc
     4981 cggggtgcgg ggaaagccgt cgagcacagc accagcctta cagtccggac gagagatgcg
     5041 gtcctttatc atgttgatgg ttacatcatc tggaacaagc tcgcctttgt ccatgaagga
     5101 tttggcgagc tttccaagtt ccgtttggtt cttaaggttc tcgcgaaaga ggtcaccgga
     5161 tgaaacgtgc actaacccgg tctcttcgga aagaattcgt gcctgcgttc ccttaccaac
     5221 accaggaggt ccgagcaaga cgatataggt tggcatttgt atttacctct agcggacgag
     5281 caaagagtcc tgatagccgt gcaatttcag ctcggcatcg atgttctgga aggtatcgcg
     5341 aacgacaccc accacaatga gcaagcccga agaagtaata aggaagatac ctgtgctgtt
     5401 ggaagcagaa atattaaagg ctgctagcaa caacccaagc aagaaaggca taatagcgac
     5461 aataccaagg aagagagcgc ccacgaaggt gatgcggctc tgaaccgtgc tcaagtattt
     5521 ttgagttggc gcacccgtta acacaccggg gacgctagct ccaacctttt tcaggttttc
     5581 gccataattt tgttggtcaa acaacacggt tgtatagaag aaagtaaata ccacaaccat
     5641 gaagaagtag atcgtccaat aaccccaatt gctggtgcca gtggcgccaa agaagttttg
     5701 aattccaaca aagaaattag caactgcttg attttcgctc tgtgtaaagt agctagcgat
     5761 aatcgcgggg aattgaagga tggcactggc aaagatcaag gggatcatgc cagaaaggtt
     5821 aaccatcaac ggcatggtgc ccttaaccgg catggacatg cggttgccaa cgcggcgacc
     5881 agggtacatg acgggcacat tgcggcgtcc ttgttggacg tacacaatgg cgagtacggt
     5941 tagcacaatg atgatcacgg tcagggtcaa cataatccag cgattggttt catctgcaag
     6001 caaagaagca aagttcgcag ggatgcgagc cacgataccg gcaaaaatga cgagggaaag
     6061 accctgtccn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6121 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnna taatgatctg
     6181 ggcagtaatg tatggataaa cacccatcga caacaacgag aagttggaga ccgtaccgcc
     6241 agagagcaag tccagaaagc caaggaagcc gcccgaacca gatgtagaac tgcggaatgc
     6301 agcaaggatg tcatggtcca caccaggagc cgggatattc gcggcaaggc gataaatcac
     6361 caagatcaca aaggtgacga tcaacttgcg gcgaatatct tccgatttcc agaggtagtg
     6421 ccagcctgaa aaggtggtct tcataggaag gttcctcgaa ttaagcgatc aattcaaccg
     6481 agccgccagc cttttcgatc ttggctttgg caccggcgct cacacgatgg acgcgcactt
     6541 tcagggcaac gctcatctca ccgcgtccca aaataaccac ggggttgcgg gtgtcgcgca
     6601 gaagatgcgc ttcagcgagg gactcaggat tcacttctgc attggctttg aaagcctcag
     6661 aaagctggtc caagttcact tcattaaatt caacctgatt gggcggagtg aagccttcac
     6721 cgcgcatgaa cggcaagcgg cgatagaaag gcaggttacc gccttggcga tacaagtgac
     6781 cgccttcacc agagcgcgca cctgcaccct tggtaccgcg accggcagtt ttaccctgac
     6841 cggcagaaat accgcgtcca acgcgttttc ggttcttttt agacccagga ttgggtacaa
     6901 gatcgtgtag tttcatgttc aacctactct tcaatcttga tcaagtgaat gatcttattc
     6961 aacataccac gcaaagcggg cgtgtctttg tgttcaacag tttggtgcat tcggcgaaga
     7021 ccaagcgcct taacggtcgc cttctggtct ttggtgtaac caatggcgct gcgcaccaag
     7081 gttacgcgaa tgctcttacc agaagtattc ttaggcatgc tgcttcctcc gttcccaata
     7141 agggagcaat tcttcagcct tcttgccgcg gcgagcagct tcgtttgccg gtgagcgtag
     7201 cccatccaag gcatcaaagg ttgccatcac cacattaagg acgttcgcgc ttcccatgga
     7261 tttggtgagg atgtcgcgca cgccagctac ttccaacacg gcgcgcacac cacctgcagc
     7321 gataacgcca gtaccagcgg aagcgggctt gaggaatact ttagcgccaa ctactttacc
     7381 gagcgactca tgtgggatgg tcgttccggc aatgttgacg gtgc
//
LOCUS       DAOQJE010000043         7414 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_5494_length_7414_cov_6.048104, whole genome shotgun sequence.
ACCESSION   DAOQJE010000043 DAOQJE010000000
VERSION     DAOQJE010000043.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 7414)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7414)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7414
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5494_length_7414_cov_6.048104"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(11..802)
                     /locus_tag="PLT08_02010"
     CDS             complement(11..802)
                     /locus_tag="PLT08_02010"
                     /inference="COORDINATES: protein motif:HMM:NF012720.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit 3"
                     /protein_id="HNE03265.1"
                     /translation="MAHSQDKSSALGQGVVIFVYLAVLTLIEYFIAVTFNATALLLVV
                     AVIKAALVVYYYMHIYELNAESGEHEDENSYEYKTGTNRIGLWLFLLSDAFVFAGLLV
                     MRSNLLGLTRPHLNQFLGLGVTAVLLISSFFMNRGETAMKHGDVKAFMNNTIITFVLG
                     LGFLLGVVLVEWRLAAEEGLVASFGNPSVGPLGGVFYMMTGMHAFHVFTGLIFLFVVW
                     NNARKGVYSAEKHWGVEAAAVYWHFVDLVWIFFYPALYLIGTAVK"
     gene            complement(822..2714)
                     /locus_tag="PLT08_02015"
     CDS             complement(822..2714)
                     /locus_tag="PLT08_02015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0009060 - aerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase subunit I"
                     /protein_id="HNE03266.1"
                     /translation="MKGFFSSALARGLFWQLIGTLVGAGLVTGIRALMGLNALDTFFF
                     TEPAWVLGGFFGALFFLAGSGVVSDWFKWARGIDTPDHHEDHHTGAAKYINVSLDHKV
                     IGVQYTITALLLIAIGGLFALIFRTELAASQLQFLTTDMKLFSQNGPQLYNTLMSLHG
                     MIMIVSILLGTAGFINYVVPLLLGAQDMAFPRLNAFSFWIAVPGAVLLLMSLFLGGFD
                     TGWTGYPPLSARAPVGMQMFFLGVFVAGWSSILGSLNVIVTVLRMRAKGMTAWRMPIL
                     IWASLATALIALTATQLIGLSFQLVMFQRLFGMGFFDPAKGGNPVLFQHLFWFYSHPA
                     VYVFILPGLGVISELLPVFVRKPLFGYRWIAMSSLGIALVGFVVWAHHMYTSGMNEYL
                     RVPFMYSTLLVAVPTGVKFFSWVATIWKGKISTPTPMLFVLGGIIVFLLGGLSGPPNA
                     TVSTDLQLQDTYWIVGHFHDTIFGGFVFPFFAAIYFWFPKATGRKMNETLGKIHFWLM
                     TPSFFALTLGMMYAGLLGMRRRIVDYDPALGIDSIHLVMTIGAFLIALSVLIFFFNFF
                     NSIKNGEKAEGNVWNSRSPEWQVPSPMPAHNYDKPFEVIGEPYDYGLNDGGFVKFIEP
                     AKNSKH"
     gene            complement(2736..3746)
                     /gene="coxB"
                     /locus_tag="PLT08_02020"
     CDS             complement(2736..3746)
                     /gene="coxB"
                     /locus_tag="PLT08_02020"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR02866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit II"
                     /protein_id="HNE03267.1"
                     /translation="MAHFVIVGILVIVMTILTYFGLDAAGLATQMHPVAASAQAAPID
                     QLWHWEIIVISFLLSLIVVPIVYSLVVFRQKKGELKDGEHIEGNTALEIGWTVVPLLI
                     VVVFAYLGAYSLGEVRRVDPNAMVVNVKAQQFSWTFEYPEYGFITTQLYLPIDRQVVL
                     KMESADVIHSFWVPEFRIKQDVVPGRITEYRVTPSLEGSYKVRCAELCGTSHAYMEAP
                     VVVVNSDAYDAWIVEQIKIAEESKTPEGQGKLLATKNGCVGCHSADGTAMTGPTWFNL
                     YGSEVKLADGSTVTADDAFITESILQPTAKVVNGFPPVMPPYTLTDEEISYIIAYIKT
                     LK"
     gene            complement(4015..4479)
                     /gene="bcp"
                     /locus_tag="PLT08_02025"
     CDS             complement(4015..4479)
                     /gene="bcp"
                     /locus_tag="PLT08_02025"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-dependent thiol peroxidase"
                     /protein_id="HNE03268.1"
                     /translation="MPVTSGIPAPTFELLDDTNTLRKLSDYRGKNVVLYFYPKDDTPG
                     CTKEACNFRDDYSAYERAGVVILGISPDSVESHVKFKKKFQLQFPLLADDGHKVCDLY
                     QVWGPKKFMGKAYEGVLRTTFLIDADGNIKKVYEDVRPAEHSQELLAELGLK"
     gene            4699..4783
                     /locus_tag="PLT08_02030"
     tRNA            4699..4783
                     /locus_tag="PLT08_02030"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4733..4735,aa:Leu,seq:tag)
     gene            4822..>5612
                     /locus_tag="PLT08_02035"
     CDS             4822..>5612
                     /locus_tag="PLT08_02035"
                     /inference="COORDINATES: protein motif:HMM:NF017508.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HNE03269.1"
                     /translation="MNIEKQFQDNHSVKLVVEVDQEKMGEYKKRAASKISQRGNIAGF
                     RPGKAPYHIVARTYGEAAITEQAIDLFIDAEYSNILTEAGVNPGASGSLENIESMDPP
                     KFLFSVPLAPEVDLGDYTSVRLPYEWKAPEQKDIDAAIEDLRQMYASTETVERAIESG
                     DYVLLDVTSETTELNRTGFATFVRSEDRDTEWPYNGFAKELIGLKAGESKTIKHSFPK
                     DWEVEDLQGKDVEITATIKTVRGVTLPEINDEFAKTTGAGETVDA"
     assembly_gap    5613..5712
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <5713..6072
                     /locus_tag="PLT08_02040"
     CDS             <5713..6072
                     /locus_tag="PLT08_02040"
                     /inference="COORDINATES: protein motif:HMM:NF017509.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE03270.1"
                     /translation="ETDVTPVAKKRLERGLILDEIVRKEQIQIDDDALNAEYMNAINS
                     LAMQGMDFNKIRGGKKGQQELSQAIAMDAASRVMTRKALDMLKSIATGSYKPFEEREA
                     EAKKAAEAETSEEKAAE"
     gene            6247..6858
                     /locus_tag="PLT08_02045"
     CDS             6247..6858
                     /locus_tag="PLT08_02045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease proteolytic subunit"
                     /protein_id="HNE03271.1"
                     /translation="MIPDFKNVVPMVVENTGRGERAYDIYSLLLRNNIIFLGTPIDDQ
                     VANVIVAQLLFLNHEDPEKEIRMYINSPGGVIYAGLAIYDTMQIISNPISTVAVGVTA
                     SFGTVLLAAGTKGRRYALPHATVHMHQPLGGAQGQATDIEIQAKQILRLKSLLNGIMA
                     KNTGQPLEVIERDLDRDYYLDAQQAVDYGLVDQIIETPEKAFK"
     gene            6992..>7414
                     /locus_tag="PLT08_02050"
     CDS             6992..>7414
                     /locus_tag="PLT08_02050"
                     /inference="COORDINATES: protein motif:HMM:NF024740.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE03272.1"
                     /translation="MIPSNIKALILDMDGVIWKGDAPIGDLPATFKRIRERGLKFVFA
                     TNNGTKTPEDYQEKLAELGVDVDPSQVVTSALGVAFMLAQRHPRGTKVFMIGENGIRV
                     ALEEKGFEVVGVEDAPQAEAVVMGIDRGINFQKMAEATL"
BASE COUNT         2174 a         1799 c         1759 g         1582 t
ORIGIN      
        1 ggtattcatt ttattttaca gccgtgccga tcagatacaa ggcagggtag aagaaaatcc
       61 aaactaaatc tacgaagtgc caatagactg cagcggcttc tacaccccaa tgtttttcag
      121 cagagtacac acctttgcgg gcgttattcc acacaacaaa gaggaagatc aaaccagtga
      181 aaacatggaa ggcatgcata ccggtcatca tgtagaaaac gccgcccaac ggaccgacag
      241 acgggttgcc aaatgaagcc accaaacctt cttcagctgc caagcgccat tccacgagca
      301 ccacgccaag caaaaagccc aagcccaaaa caaaggtgat gatggtgttg ttcataaagg
      361 ctttcacatc gccatgtttc atagcagtct cgccacggtt catgaagaag gaagatatga
      421 gcaacacagc cgttacaccc aaacccaaga actggttgag gtgcgggcgg gtaagcccaa
      481 gcaagttaga acgcatcacc aacagaccag caaacacaaa tgcgtctgac aagaggaaca
      541 gccaaagacc tatacgattt gtgccggtct tatactcata agagttttca tcttcatgct
      601 cgccgctttc tgcattcaac tcatagatgt gcatgtagta atacacaacc aaagcggctt
      661 tgatcactgc cacaaccagc aaaagggctg ttgcgttgaa tgttacggca atgaagtatt
      721 caataagggt cagaactgcc aaatatacaa agatcaccac gccctgacca agagcggacg
      781 atttatcttg tgaatgtgcc atttaattac tctcctcgcc tttagtgctt gctattcttg
      841 gcaggttcaa tgaacttaac aaagccacca tcattcaaac cgtagtcata cggttcgccg
      901 atcacttcga atggcttatc gtagttatgg gcaggcatgg gggaaggaac ctgccattca
      961 ggggagcgcg aattccaaac gttgccttcc gccttctctc cattcttgat gctattgaag
     1021 aagttgaaga agaaaatcaa aacggaaagg gcgatcagga acgcaccaat ggtcataact
     1081 aaatgtattg agtcaatacc caaagcaggg tcatagtcca cgatgcggcg gcgcataccg
     1141 agcaagccag catacatcat gcccaaggtt aaggcaaaga acgaaggggt catcagccag
     1201 aaatgaatct tgccaagggt ttcgttcatc ttgcgtccgg ttgctttggg gaaccagaag
     1261 tagatggcag caaagaatgg gaagacaaac ccaccgaaaa tggtgtcgtg gaagtgccct
     1321 acgatccaat aggtgtcttg taactgaagg tcggtagaga ctgtcgcatt ggggggaccc
     1381 gagagaccac ctaaaaggaa gacgataata ccgcccaaca caaagagcat tggggtagga
     1441 gttgaaatct tgcctttcca aattgtcgca acccaggaga agaacttaac gcctgtcggt
     1501 acagccacca acaaggtgct gtacatgaaa ggtacacgta aatattcatt cataccagag
     1561 gtatacatgt ggtgtgccca cacaacgaag cccaccaaag caatgcccaa agaggacatc
     1621 gcaatccagc gataaccgaa gaggggcttg cggacaaaca caggcagtaa ttcagagatc
     1681 acacccaagc caggcaaaat gaatacgtac accgcagggt gagagtagaa ccagaacagg
     1741 tgttggaaga gaaccgggtt gccgcctttg gcagggtcaa agaagcccat tccaaagaga
     1801 cgttggaaca tcaccaactg gaaggaaaga ccgatcaact gtgtagcggt cagcgcgatc
     1861 agtgcagtgg caagagatgc ccaaatgagg atcggcatgc gccaagcggt catgcctttg
     1921 gcacgcatac gaagcacagt aacaataacg ttcaaagaac caagaatgga tgaccaaccg
     1981 gcaacgaaca cgccaaggaa gaacatctgc ataccgacag gagcgcgggc agacagaggc
     2041 gggtaacccg tccaaccagt atcaaaaccg ccgaggaaaa ggctcatgag cagaagcaca
     2101 gccccaggga cagcaatcca gaatgagaag gcattgaggc gcgggaatgc catatcctgc
     2161 gcaccgagca acaggggaac gacatagttg atgaagccag ctgtgcccaa caagatcgag
     2221 acgatcatga tcatgccatg caaggacatc aatgtgttgt acaactgagg tccattctgg
     2281 ctgaacaatt tcatatcagt agtgaggaat tggagttgag atgcagccaa ttctgtacga
     2341 aagatcaaag caaataaacc gccaatggcg atcaacaaca acgctgtgat ggtgtactga
     2401 acaccaatga ccttgtggtc aagtgaaacg ttgatgtact ttgccgcgcc ggtgtggtgg
     2461 tcctcatggt gatctggcgt atcaatgcca cgcgcccact tgaaccaatc gctcacaacc
     2521 ccagagccag cgaggaagaa aagtgcaccg aaaaagccgc ccaacaccca ggctggttcg
     2581 gtaaagaaga acgtatcaag cgcattcaac cccatcaatg cgcgaatgcc agtgacaaga
     2641 ccagcaccaa cgagcgtgcc gatcaattgc caaaacaaac cgcgagcaag ggcactagaa
     2701 aagaatccct tcatagtatt taatccttat caaatttatt tcaaggtctt gatgtatgcg
     2761 atgatataag agatctcttc atcggtcaaa gtgtaaggcg gcataacggg cgggaagcca
     2821 ttaacaacct tagctgttgg ctgaagaata gactctgtga tgaaggcatc gtcggcagtc
     2881 acagtggagc cgtcagcaag cttaacctct gagccataca ggttgaacca agtcggtcct
     2941 gtcatagctg tgccatccgc agagtgacaa ccaacacagc cattcttggt tgcaaggagt
     3001 ttaccctgcc cttcgggggt cttggactct tcggcaatct tgatttgttc aacaatccag
     3061 gcatcatacg catctgaatt gacaacgaca acaggggctt ccatataggc atgggatgta
     3121 ccgcaaagct cggcacagcg gactttgtag ctgccctcaa gactcggagt aacacgatac
     3181 tcggtgatgc gcccaggtac aacgtcttgt ttgatgcgga actcaggaac ccagaacgag
     3241 tggatcacat ccgcagactc catcttaagc acaacctggc ggtcaatggg cagatagagt
     3301 tgagttgtaa taaacccata ttcggggtat tcaaaagtcc atgaaaattg ttgggctttg
     3361 acgtttacaa ccatggcgtt tgggtctacc cggcggactt caccaagtga ataagcgcct
     3421 aaataagcaa aaacaaccac gatgagtaat gggacaaccg tccaaccgat ttcaagggca
     3481 gtattcccct ctatatgttc cccatcctta agttcgcctt tcttttggcg aaacacaacc
     3541 aaactatata caatcggtac aacgatcagc gaaagtagga aggaaattac gatgatttcc
     3601 caatgccata actgatcaat gggagcagct tgtgcgctgg ctgccacagg atgcatttgg
     3661 gttgcaagcc cagcagcatc aagaccgaaa tacgtgagaa tggtcatcac aatgaccaag
     3721 attcccacaa ttacaaaatg ggccatatac tctctctcct gaaaagattg aacggggcat
     3781 gagctccgat gattaactaa aattggacaa atacagttag aattgtctca attttcacaa
     3841 tatcagatta taacaaaatc cctttttatt gtcacgctat cttggtcaaa tgcacagcta
     3901 ttcttaactt gcacaaacct caattttcac aaatcatgcc gacggtgttt ggataccgtc
     3961 ggcaatctaa aagctatatc aagctataac acgatacgtt gtcgtagaga tgcgttattt
     4021 caagcctaac tctgctaaaa gttcctggct gtgttcagct gggcgcacat cttcatatac
     4081 tttcttgatg tttccgtcag cgtcgatcaa aaacgttgta cgaagaacac cttcatatgc
     4141 tttacccata aactttttag gtccccacac ctgataaagg tcgcaaactt tgtgtccatc
     4201 atcagcaagc aagggaaatt gaagctggaa tttcttttta aacttcacat gagattcaac
     4261 ggagtctggg ctaatgccta gaatcaccac ccctgccctt tcgtaggcag agtaatcatc
     4321 gcgaaagttg cacgcttctt ttgtgcaacc gggcgtatca tctttcggat aaaaatataa
     4381 gacgacattc tttccacgat aatcggaaag tttgcgtaag gtattggtat cgtcaagcaa
     4441 ttcaaatgta ggggcaggaa tgccagaagt aacgggcata aaaaagtact ctctctccaa
     4501 agaagtatgc gcgttattat acctttattc attttaaggg taggaaaact ttcacaatta
     4561 tttggtgggc agcactccaa ttttcggtag aaaaccggct ttaagcatgg atcaatctcc
     4621 ttataaaaaa cctcagaaaa aatggcgatt cgggcattcc ctacggtata attgcgcccg
     4681 cctgtaagca cacaaaccgg ggatgtgctg gaactggcag acaggcatga cttaggatca
     4741 tgtgccgcaa ggcgtgaggg ttcgaccccc tccatcccca ccaaaataat attcagacct
     4801 tattggtctt cggaggaacc attgaacatc gaaaagcaat ttcaagataa tcactcagtt
     4861 aagctggtcg tagaagtaga ccaggagaaa atgggcgaat ataaaaagcg cgcagcgagc
     4921 aaaatttctc aacgcggtaa tatcgccggc tttcgccccg gcaaagctcc ttatcacatc
     4981 gtagcccgca cctatggcga agcggccatc accgaacaag ccattgactt gttcatcgac
     5041 gcggaatatt ccaacatttt gaccgaagcc ggtgttaacc ccggcgcttc tggcagcttg
     5101 gaaaacatcg aaagcatgga tccacccaag ttcctcttta gcgtgccgct cgcacctgaa
     5161 gtggacctcg gtgattacac ctcggtacgc ctcccctacg aatggaaagc ccctgaacag
     5221 aaggatattg acgccgcgat tgaagacctg cgccaaatgt acgcctccac cgagaccgtg
     5281 gaacgtgcca tcgaatctgg agattatgtg ctcctcgatg ttacctctga gacaactgaa
     5341 ctcaaccgca ctggctttgc tacgtttgtc cgcagcgaag accgtgacac tgaatggcca
     5401 tacaacggtt tcgccaagga acttattggt ttgaaggctg gcgaaagcaa gaccatcaag
     5461 cacagcttcc ccaaagattg ggaagtggaa gacctgcaag gcaaggatgt ggaaatcacc
     5521 gcaaccatca aaaccgtgcg cggcgtgacc cttcctgaaa tcaatgatga attcgccaag
     5581 acgaccggcg ctggcgaaac tgtcgatgca ttnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5641 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5701 nnnnnnnnnn nngaaaccga tgtgaccccg gtggcaaaga aacgcctcga acgcggtttg
     5761 atccttgatg agatcgtgcg caaggaacag attcaaattg acgacgatgc cctgaatgca
     5821 gaatatatga atgccatcaa cagcctcgcc atgcaaggta tggactttaa caagatccgc
     5881 ggcggaaaga aaggtcaaca agagttgagc caagccattg ccatggatgc ggcaagccgt
     5941 gtgatgaccc gcaaggcgtt ggatatgttg aagagcatcg cgaccggtag ctacaagccg
     6001 tttgaagaac gcgaagccga agccaagaaa gccgctgaag ctgaaactag cgaagaaaaa
     6061 gccgcagagt aagaaaaggc gtttcgaagg ctggcaggct taggtccaag aaatgaagcg
     6121 tgcgccgcgt cccatcgggt agaatcctct catcgaaccg ggcgcggcgc atttatatcg
     6181 cctatcaaac tctaatataa atccaatagt ccctcggtaa cattggtcga ataaaggaga
     6241 ccgaacatga tcccagattt caaaaatgta gttcccatgg tggttgaaaa tacaggtcgc
     6301 ggcgaacgcg cttatgacat ttactcgctg ctcctgcgta acaacatcat cttcctcggc
     6361 acgcccatcg acgaccaggt cgcgaatgtg atcgttgccc agcttttgtt ccttaatcac
     6421 gaagaccctg aaaaagaaat ccgtatgtac atcaactcgc ctggcggtgt gatttatgcg
     6481 gggctcgcaa tctatgacac gatgcagatt atctcgaacc ccatcagcac cgttgccgtg
     6541 ggtgtgaccg cctctttcgg gaccgtgttg ctggctgctg gaaccaaggg tcgacgctac
     6601 gccctgcccc atgccaccgt ccacatgcac cagcctctcg gcggcgcaca aggtcaggca
     6661 acggatatcg aaattcaggc gaagcagatt ttgcgcctca agagcctgct caatggcatt
     6721 atggcgaaga acaccggtca gccgctcgaa gtgatcgaac gcgacctcga ccgtgattac
     6781 tatctcgatg ctcaacaggc tgtggattac ggcttggtgg atcagatcat cgaaacccct
     6841 gaaaaagcat tcaaatagtt attaatacgg tagctatgag acctcgaaga gaaatcttcg
     6901 gggtctttct tttataggta taatgcccgc tgcgttgttc gtgatacgta aaccgtacat
     6961 cgaaaatcta aaaccgcaaa ctgtaaattg tatgattcct tcaaatatca aagcacttat
     7021 tctcgacatg gacggtgtga tctggaaagg tgacgccccc atcggtgact tgcccgctac
     7081 gttcaaacga atccgtgagc gcgggttgaa gttcgtattc gctacaaata atggaaccaa
     7141 gacgcctgaa gattatcaag aaaagcttgc cgaactcggc gtggacgtgg acccttcaca
     7201 agtcgtcaca tcggcattag gcgtggcatt catgttggca cagcggcatc cgcgtggaac
     7261 gaaggttttt atgatcggtg agaatggcat ccgcgtggca ttggaagaaa aggggtttga
     7321 agtggttggc gtggaagatg cgccacaagc tgaagccgta gtgatgggca ttgaccgcgg
     7381 cattaatttt caaaaaatgg cagaagcaac tctg
//
LOCUS       DAOQJE010000044         7386 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_5529_length_7386_cov_7.084572, whole genome shotgun sequence.
ACCESSION   DAOQJE010000044 DAOQJE010000000
VERSION     DAOQJE010000044.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 7386)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7386)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7386
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5529_length_7386_cov_7.084572"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1130
                     /locus_tag="PLT08_02055"
     CDS             <1..1130
                     /locus_tag="PLT08_02055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE03273.1"
                     /translation="SQKLVYNSIYMQSPRNTFIPIFLRLTSIVSLVSILVSCQLSPSI
                     TNTAEAPTEIADQTIPVLPETFQTDLLNPVDVPHTYVEETCRYLRNKWNPVNAQPGTV
                     VMLILIKDINRGAVELPNSIRITDFYDLLEQLKAQGFEAINTEKLQAFLERNLPIPPR
                     SVVLIQDGNHTEEYFDKNYRWYYEQWGWTVVNGFVSDPNIDPILREGNFSLEYEGFID
                     HQARGITPDVVLTDDIAKTIIARELQGSLEGFAEQLGKTPNAIIWPNGGFGIRPIEAA
                     RQLRFKLGVTQNPRGPVMYNWVPLANSVDDARPTLIPEGKVGDPLMTLPAYSPQEAFN
                     AIDSVRNIGNAAQAYAESNKDAEYQYYDIVCSDKYGPIPTP"
     assembly_gap    1142..1241
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            1356..2765
                     /locus_tag="PLT08_02060"
     CDS             1356..2765
                     /locus_tag="PLT08_02060"
                     /EC_number="2.1.1.148"
                     /inference="COORDINATES: protein motif:HMM:NF014560.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050797 - thymidylate synthase (FAD)
                     activity [Evidence IEA];
                     GO_process: GO:0006231 - dTMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent thymidylate synthase"
                     /protein_id="HNE03274.1"
                     /translation="MPEREIYLLSPRALSPETIAVAFAKTSRAPESFREIAAELSDES
                     SAKFHEKWVVGYGHASVAEHAVLHIAFENVSRVAIETIEGNRLASYTEKSTRYQKWGS
                     DDFTIPPELDGHPLRDEFIATVRLLFSTYAESLDPVKNLIVERTPRRENEADEAYDRR
                     IRSQYVDRCRFLLPAAANANVGMTANARVIEMTIRKMLSHPLEEVRQIGEKLKEVSKG
                     ETPTLVKYADANPYTVETIRDLENRPSQIENRKSDWCELIEYDKDGEKKILAAALYRF
                     DEIPYADALMYVKTLKKKEREALAETLLGKLGKYDTPLRELEYSNYTFDLVMDQGAYA
                     EFKRHRMMSQTPQRLTTRLGYTTPLLITEAGFGSKYEAAMEAASQMYEKLHAFNPDVA
                     QYIVPNGFNRRVLAQFNLREAFAFCQLRTAANAHFSIRRVAQKIYEDISEVHPLLSKY
                     MKLREETWQSVEENYFSKI"
     gene            complement(2806..3792)
                     /locus_tag="PLT08_02065"
     CDS             complement(2806..3792)
                     /locus_tag="PLT08_02065"
                     /inference="COORDINATES: protein motif:HMM:NF012472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HNE03275.1"
                     /translation="MQYRHLGRTGIQVSELSIGSWVTFKNQVDVQAAVEIMAAAYDHG
                     VNFYDNAEVYAGGKSEEVMGAALKKLNWRRGSYLVSTKFYWGIEEGVNEKNTLNRKRL
                     IEGINGALKRLDMDYVDLIYCHRPDKTTPIEETVWAMHNIIEWGKALYWGTSEWAASE
                     IIEAIQIAERHHLHKPVVEQPQYNLFERKRLEGDYVRFYKEYGYGTTIWSPLASGLLS
                     GKYNKGIPADSRGALKGYEWLHGSLTNQEKLAKVAGLEPIAKELGGTVAQLAIAWCLK
                     NPYISTVITGASRVEQVHENMKSAEIAAKLTPEVMGKIDVVFGVQKKDEDDD"
     assembly_gap    3870..3969
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(4165..5781)
                     /locus_tag="PLT08_02070"
     CDS             complement(4165..5781)
                     /locus_tag="PLT08_02070"
                     /inference="COORDINATES: protein motif:HMM:NF012706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HNE03276.1"
                     /translation="MDKFKTIHRRFTVGGLALVLLASLFLTASAPSDPDFQIGVPLDQ
                     AIVYSGGESSNLRDYDPATTYSAGDKLIFSGLVQLDQNLNIRPDLAETWEVTGDGTIY
                     TFKIRNNAKFHNGRDVTAQDVVFSWERAASPELASDTVLTYLSDIVGVREKALGQADS
                     ISGLKVIDDKTLQVTIDAPKPYFLMKLTFPTSYIVDQDNVSAGEEWVRQPNGTGPYKL
                     KEWRSNEYIVYEANPDFYLGAPSIPYVVFKLYAGDDVRLFETGDVDIASVGLYDVERM
                     LNPNEPLNRNLVTGVDLCTSYVTFDTTQPPFDDANVRKAFSMAFDRQRYVDVLYRGVA
                     LPAEGLYPPGLPGFDYNLQGLPFDPEQARELLKQSKYGGPEGLPEIIYTNAGIGSYIG
                     GNVAALAEMWQDYLGVKIKVENLDYNFYYQQIFSGNHGQIFDGGWCADYPDPENFADV
                     LFHTGVPQNQGGYSNPELDTLLEAARIEPDVTKRIAMYQEAERIIVNDAPVIFTTHSL
                     SYQLVQSYVKGYVNTPISISAERFMWLEGK"
     gene            complement(5774..7135)
                     /locus_tag="PLT08_02075"
     CDS             complement(5774..7135)
                     /locus_tag="PLT08_02075"
                     /inference="COORDINATES: protein motif:HMM:NF024876.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase MA family metallohydrolase"
                     /protein_id="HNE03277.1"
                     /translation="MHKKLILFLTLLTILLMPAKVQAALHADVQADTATLDFPNTATF
                     SATLSASAEITSVTLEYGSEQLNCGEVIAKAFPDFSPSPSIDVSWTWDMRQSGSLPPG
                     TTIWWRWRYVDSAGKEYLSDTNTVIWLDNDHDWQTATGTNIRIHYYGASQSFAQSMVD
                     AGLEGLRRNKDQAGMTPDIPVDIYVYPSYDEMRDAILYEPQWTGGLAYSNFSIIIMGT
                     SGDANFDKATVIHEVTHVLVGRNAFTCIGFIPTWLNEGLAVYNEGPLDESMKAQFDIA
                     LRDDTFFPVRSLSGNFSEIPEKALLSYAQSHSVVKFMLETYGQEKMSQLLQALSDAEP
                     VDDALVQIYGVDTDGLDAAWRQSLGLQPLVVAQATSQPTPTYVPTYVPIAGIPQAITA
                     TPGAIPTSSSTNNSEPSFSGPPIVLTVVMLCICLVFLTIIGVVVLGIVLRNNKGNGGK
                     KNG"
BASE COUNT         1968 a         1784 c         1628 g         1806 t
ORIGIN      
        1 attctcaaaa actggtttac aattctattt atatgcaatc gccaagaaat acgtttattc
       61 ccatctttct tcgcctcaca tccattgtct cactcgtttc cattctggtt tcctgccagt
      121 taagtccatc tataacaaat acagcagaag ctcctactga aatagcagat caaaccatcc
      181 ctgttttacc agaaacgttt caaaccgatt tattaaaccc cgttgatgtt ccacatacct
      241 atgtagaaga gacctgtcgt tatttacgca acaagtggaa ccctgtaaat gcgcaaccgg
      301 gcaccgttgt catgctcatc cttatcaagg atataaatcg tggcgccgta gaacttccaa
      361 atagtattcg catcacagat ttctatgact tattagaaca attaaaagca caggggttcg
      421 aagcgatcaa tacagaaaaa ctacaagcat ttctggaacg gaaccttccc atccctccgc
      481 ggtctgttgt attaattcaa gatgggaatc acacagaaga atatttcgat aaaaactatc
      541 gctggtatta tgaacagtgg ggctggaccg tcgtcaatgg gttcgtcagt gaccctaata
      601 tagaccctat cttacgcgaa gggaatttct cgcttgaata tgaaggcttc atagaccacc
      661 aagcgcgcgg catcacaccc gatgtagttc tcactgatga cattgcaaaa acaattattg
      721 cccgtgagtt gcaaggctct ttagaagggt ttgcagaaca attaggcaaa acaccaaatg
      781 ccattatctg gcccaacggc gggtttggaa ttcgtcccat agaagcagcg cgacagttac
      841 gcttcaagct gggagtcact caaaacccac gcggacctgt catgtacaat tgggtaccgc
      901 tcgcaaacag cgtcgacgat gcacgaccaa ccttaatccc tgaaggcaaa gtcggtgacc
      961 cactcatgac cttacccgct tactcaccac aagaagcatt caatgctatt gacagtgtcc
     1021 gtaatatcgg gaatgcggct caggcatatg cagaatcaaa caaagatgcc gaataccaat
     1081 attatgatat tgtttgttcc gataaatatg gtcccatccc gaccccataa ataggaggct
     1141 annnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1201 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn ncacctgctc ggcggcagga
     1261 caatgcgagc cttagggtaa aatcatcgaa gcgatcagcg cacaacgggc tggtcgcttt
     1321 tttatgaaat agattcaaca catacaggag acaccatgcc cgaacgagag atataccttt
     1381 tatccccacg cgccctcagc ccagagacta ttgccgtagc atttgcaaaa acatcccgcg
     1441 cccctgaatc tttccgcgaa atcgccgcag aacttagcga tgaatcttca gcaaaattcc
     1501 atgagaaatg ggtcgttggt tatggtcatg cctctgtagc cgaacatgct gtcctgcata
     1561 tcgcgtttga aaatgtctca cgtgttgcca ttgaaacaat cgaaggcaat cgccttgctt
     1621 cttatacgga aaagtccact cgctaccaaa aatggggctc cgatgacttc accattccgc
     1681 cagaattaga cggacatcct ctgcgcgatg aattcatagc aaccgtccgc ctccttttct
     1741 cgacctatgc tgagtcattg gacccggtca agaatctcat cgtcgagcgg actccccgcc
     1801 gtgaaaacga agcggatgaa gcctatgatc gccgtatccg ctcacaatat gtggaccgtt
     1861 gccgctttct gctgcctgcc gccgccaatg ccaatgtagg tatgaccgcc aatgcgcgcg
     1921 tcatcgaaat gaccattcgc aaaatgcttt ctcacccact cgaagaagta cgtcaaattg
     1981 gcgagaagtt aaaagaagtc tctaaaggcg aaacacccac cttagtcaaa tatgcagacg
     2041 ctaatccata cacagtcgaa accattcgtg atttggaaaa tcgtccatct caaatcgaaa
     2101 atcggaaatc agattggtgc gaacttattg agtacgacaa agatggagaa aagaaaattc
     2161 tcgccgcggc gttataccgc tttgatgaaa tcccctatgc cgatgctctg atgtacgtca
     2221 aaacgcttaa gaaaaaagag cgtgaagctc tggctgaaac cttgcttggc aaactcggca
     2281 aatatgatac ccctctgcgt gaattggaat attccaacta cactttcgac cttgtcatgg
     2341 accaaggcgc atatgccgag ttcaaacgtc accgtatgat gagccagacc ccacaacggc
     2401 tgaccacaag gctcgggtat acgactccgc ttcttataac ggaagcaggt ttcgggtcca
     2461 agtatgaggc ggcgatggaa gcagcatcac agatgtatga aaagctccat gcgttcaatc
     2521 cagacgttgc ccaatacatc gtcccgaacg gatttaatcg ccgggtgttg gcgcaattca
     2581 atttacggga agccttcgcc ttctgtcagt tgcgcacggc tgccaatgcc cacttttcaa
     2641 ttcggcgcgt ggcacaaaaa atttacgaag acatttccga agttcatcca ctgctttcaa
     2701 aatatatgaa actgcgtgaa gagacttggc aaagcgtgga agaaaattat ttttcgaaga
     2761 tctaaacaaa aactccgagg tcttgaaacc tcggagtttt tttatttaat cgtcgtcttc
     2821 gtctttcttt tgcacaccaa agaccacatc gatcttaccc ataacctcgg gcgtgagttt
     2881 ggcagcaatc tctgctgact tcatattttc atgaacttgt tccacacggc ttgcaccggt
     2941 aatgaccgtg ctaatgtagg gattcttcaa gcaccatgca attgccagtt gcgccacggt
     3001 gccgccaagt tctttggcaa taggctccaa acctgcaacc ttcgccagtt tttcttgatt
     3061 ggtgaggctg ccatgcaacc attcataacc tttgagtgcg ccgcggctat cagcagggat
     3121 gcccttgttg tatttaccag acagcaaacc agaagcgagt gggctccaaa tggtagtgcc
     3181 ataaccatat tctttataga agcggacata atcaccttcg aggcgtttgc gttcaaaaag
     3241 gttgtattgc ggctgttcaa ctaccggttt atgcaagtga tggcgttctg caatttgaat
     3301 ggcttcaata atctcagaag ccgcccattc agaagtgccc caatacaagg ctttgcccca
     3361 ttcaataata ttatgcatcg cccaaactgt ttcttcaatg ggcgtggtct tatctgggcg
     3421 gtgacagtag atcaagtcaa catagtccat gtcgagacgt ttgagtgcac cgttgatacc
     3481 ttcaatgaga cgcttgcgat tgagggtatt cttttcgtta accccttctt caatgcccca
     3541 ataaaacttg gtggaaacca aataactacc acggcgccag ttcaacttct tcaatgcagc
     3601 gcccatcacc tcttccgatt ttccgcctgc atacacttcg gcgttatcgt aaaagttcac
     3661 gccgtgatcg tacgccgctg ccatgatctc cactgcggct tgcacatcca cttgattctt
     3721 gaatgtaacc caagacccaa tagataactc actgacttga atgcccgttc ttccaagatg
     3781 acgatattgc attgttcctc caaaagattt tatggcgaat ccgccgtatt tttcgagcac
     3841 attataatca ctgcatgcga aaaaccttgn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3901 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3961 nnnnnnnnnt tcgataaata tggaaccgtg gttggtgtgc gtcgctggga aggcggagcg
     4021 ctccagccgg gaagttcact caacttcgac tttagcgtat ccagcgtcgg cggggaaatt
     4081 gaggcggtgg agttcttcgc tcaagcaaaa taaaatcctt tcctctttct ccttatccaa
     4141 caaaaagaaa gaggaaagga aaaattattt accttccaac cacataaaac gttctgccga
     4201 aattgaaata ggtgtattca catatccctt cacatacgat tgcacgagtt gatacgaaag
     4261 cgaatgggtg gtgaagatca caggcgcatc gttgacaatg atgcgttcgg cttcttgata
     4321 catggcaatg cgtttggtca catccggttc gatgcgagcg gcttcgagta aggtgtccaa
     4381 ctctgggttg gaatagccgc cttgattttg cggcacgccg gtatggaaga gaacatcggc
     4441 aaaattttca gggtcggggt aatcggcaca ccaaccacca tcaaagatct gaccatgatt
     4501 gccagaaaaa atctgttgat agtagaagtt gtaatctaaa ttctcgacct tgatcttcac
     4561 gccaagatag tcttgccaca tctcagccaa agcggctaca tttccaccaa tatagctacc
     4621 aatacccgca ttggtgtaaa tgatctcagg caagccttcg ggtccgccgt atttggattg
     4681 cttcaacaat tcacgtgctt gttcggggtc aaagggtaaa ccttgcaggt tgtaatcaaa
     4741 gccgggtaaa ccgggcgggt ataacccctc tgctggcaat gccacaccgc gatataacac
     4801 atccacatag cgttgacggt cgaatgccat gctaaaggct ttgcgaacat tggcgtcgtc
     4861 aaaaggcggt tgggtggtgt caaaggtaac atagcttgta caaaggtcca caccggtgac
     4921 gagattgcgg ttcaaaggct catttgggtt cagcatacgt tctacatcgt ataagccgac
     4981 gctcgcaata tctacatcgc ccgtttcaaa gaggcgcaca tcatcacctg catagagttt
     5041 gaagacaaca taaggaatcg atggcgcacc caggtagaaa tctggattgg cttcgtaaac
     5101 gatatattcg ttggagcgcc attctttgag tttataaggt cccgtgccat taggctgacg
     5161 cacccattcc tcaccagcgg atacattgtc ctgatccaca atataggatg tggggaaggt
     5221 gagcttcatg agaaagtatg gcttgggcgc atcgatcgtg acctgaaggg ttttgtcgtc
     5281 gataactttt agcccgctga tactatctgc ctgaccaagc gctttctcgc gtactccaac
     5341 gatatcgctc aagtaggtga gaacggtgtc agacgccaac tcagggctgg cagcgcgctc
     5401 ccaagaaaag accacgtctt gagccgtaac atcccgtcca ttatggaact tggcattgtt
     5461 gcgaatcttg aaggtgtaga tagtgccatc gccagtcact tcccaagttt cggcaaggtc
     5521 tgggcggatg ttcaagtttt ggtcgagttg caccaaaccg ctgaagatga gtttatcgcc
     5581 cgcactatag gtcgtggcgg ggtcgtaatc acgcaaattt gaagattcac caccactgta
     5641 aacaatcgcc tgatctaacg gaacaccaat ctgaaaatcg ggatcgctgg gtgcgctggc
     5701 agtaaggaag agagaagcaa gtaaaacaag cgccaaacca ccaacggtaa agcgccgatg
     5761 tatggttttg aatttatcca ttcttctttc ctccattgcc tttattattg cgcagcacaa
     5821 ttcctaaaac cacgacaccg atgatcgtca aaaagacgag acagatacat agcatcacaa
     5881 cggtcaacac aatgggcgga ccactaaaag aaggctcgga attatttgtt gacgatgaag
     5941 ttggaattgc gcccggtgtg gcggtgatgg cttgaggtat gcccgcaata ggaacatagg
     6001 tcggcacata ggtcggcgtg ggctgcgatg ttgcttgtgc caccaccagc ggttgaagcc
     6061 caagcgactg gcgccaagcg gcatcgagcc catcggtatc cacgccgtaa atttgcacca
     6121 aggcatcatc gacaggttcg gcatcgctaa gcgcttgaag cagttgactc atcttctctt
     6181 gtccataggt ctcaagcata aacttgacca cactatgaga ttgagcgtat gaaagcaagg
     6241 ctttttctgg gatttcagag aaatttccgc tcaaagaacg gacagggaaa aaggtatcgt
     6301 cacgcagggc aatatcgaat tgcgccttca tgctttcatc cagcggacct tcgttataca
     6361 cggcgagacc ttcattcaac caggtaggaa taaacccaat acaagtaaat gcattgcgtc
     6421 ctactagcac atgggtcact tcatggatga cggtggcttt atcaaaattg gcatcgccag
     6481 aagtgcccat gataatgatg ctgaagtttg agtatgccaa accgcctgtc cactgcggtt
     6541 catagagaat ggcatctcgc atttcatcgt aactggggta gacgtaaata tcgacaggga
     6601 tatctggcgt cataccggct tggtctttgt ttcggcgtag accttccaaa ccagcgtcca
     6661 ccatagactg ggcgaaactt tggcttgcac catagtaatg gatgcgaata tttgtgcctg
     6721 ttgcagtttg ccagtcatgg tcattatcca accagatgac tgtattcgta tcgcttagat
     6781 attccttgcc agcagagtca acatagcgcc agcgccacca gatggtagtg cctggcggaa
     6841 gggatccgct ctgacgcata tcccaggtcc aggagacgtc tatggatggg ctgggtgaaa
     6901 aatcggggaa ggctttggcg atcacctctc cacagttcaa ttgttcgctg ccatattcca
     6961 gggtgactga ggtaatttct gcagaagcac taagagtagc agaaaaagta gccgtgttgg
     7021 gaaagtccaa ggtggctgta tccgcttgaa cgtcggcatg aagcgcagcc tgaaccttgg
     7081 caggcatgag caaaattgtg agtagtgtca aaaacaggat gagcttttta tgcatccttt
     7141 tcctccattc gtttttgcaa ctataccatt tttcaaagcc actttcctcc ctctgatgtg
     7201 gggttaaacc ccgtcatgga gaaataggac aaattcacgc agaattcatg acatccatca
     7261 gaccttaaac tcagccctat ttaatgtcat catgtaaaat ggacatagat tgtttgattt
     7321 agtaacacaa acctttggag acctcctatg caacgaaaca aatttatcat cggcggcgtg
     7381 ctcatt
//
LOCUS       DAOQJE010000045         7376 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_5546_length_7376_cov_5.962164, whole genome shotgun sequence.
ACCESSION   DAOQJE010000045 DAOQJE010000000
VERSION     DAOQJE010000045.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 7376)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7376)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7376
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5546_length_7376_cov_5.962164"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..406
                     /locus_tag="PLT08_02080"
     CDS             <1..406
                     /locus_tag="PLT08_02080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE03278.1"
                     /translation="LERDIELLAYHGSPKGTTHLVLATTSTETLNEYYSDQTASIFIG
                     GHSHIQMERRHGSKLFLNAGSVGNAFQFAYTPGNPPNLLPWAEYAIVEQEGNFLHADM
                     RRIYFDIDALLARVKESKLPGANWWFKQYENR"
     gene            478..1752
                     /locus_tag="PLT08_02085"
     CDS             478..1752
                     /locus_tag="PLT08_02085"
                     /inference="COORDINATES: protein motif:HMM:NF014081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HNE03279.1"
                     /translation="MTIHVLKNATLINGTGSKPIDRGLVAIEGETILFAGNMEGWQNP
                     EGKIIHEMDLTGKTILPGLIDSHVHIALECLPDSTLSGPWGWTTLIMLKHAQNTLAAG
                     ITSIRDVGGRHHLEFSLRKAIEAGLFVGPRMSLAGKLLSITTTGTEWYEGMYREADGV
                     EEVRKAAREQLKAGADLIKVLATGAVLAPGEKPGAATFELEEIRAAVIEAAKVGKIVA
                     AHAHGIEGIRNAVEAGAKTIEHGTYLHQDLRLIERMAKEEIFVVPTLKAGFDVIEGDR
                     PGIPDWILEKSKETQEDAMKSIRKAYEMGVPIAMGTDAATPYNFHGENAMELYYMSQA
                     GISAMDCIVASTKNAARALGWDSKLGTLEAGKLADLIVVKKNPLDDLRSLANRNNIEY
                     VMQGGKFVARQFVEPTGIPEELMAGAWICCGF"
     gene            1771..3096
                     /locus_tag="PLT08_02090"
     CDS             1771..3096
                     /locus_tag="PLT08_02090"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF012630.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031012 - extracellular matrix [Evidence
                     IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="matrixin family metalloprotease"
                     /protein_id="HNE03280.1"
                     /translation="MKRVYQYLSVYVFLALACSSLTPSTSTGTGYRYDNHPDANTQSA
                     EYRALSKWGKTDITYYFINGTDKISGDREQELVAQAFALWAAQTSLTFTETNSRQSAD
                     IVIGWAEGEHGDGDPFDGPGDVLAHASYPNPYEERQVFLHFDDAERWVDSDTQDVDLL
                     TVAAHEIGHALGLGHSNDPNSLMYPSYSEPHRFLGGDDIAGIQSLYGLASEPPTAPEV
                     PKPNENPPTSPSQDSDGDGLADSDETLITGTDPNNKDSDSDGIGDGVEVVNRMNPLDA
                     DMDKDGVKDGAEIAAGTNPFLPDQANGVSPELSKEVGEFLTKAIKLQIEAYRQGDASV
                     ASVIMAGDVLQSLQGEISSLNQQGLVEIADIDYYKSYIDDIRIVNNAQLEVDTCEVWS
                     LQYYRRSDGALVQSNEPTLLPQTITIQKIENGWFITAVQFFEAPAFCHS"
     gene            3299..4741
                     /locus_tag="PLT08_02095"
     CDS             3299..4741
                     /locus_tag="PLT08_02095"
                     /inference="COORDINATES: protein motif:HMM:NF019844.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HNE03281.1"
                     /translation="MFVTKNLYQNDEAWKNVPLGFSSQTIGSWGCLLTSVTMMLNGIG
                     YNETPDTVNTKMKNAGGFQGPLFIPSVLPYVWGNCAYRDMQPCESVPAPLQQIDDAVA
                     SGKPVILQVDWNKQAGIQTHFVLVKEKKGDDYVLYDPYKYGGDGPDKEVLLTTRYKYN
                     GAKLSTEISAVLWFENYGINPPEPPAKVPVPAENFILYAAEDDLALRSQPATTGYLWK
                     RMVMNTELICLEPKAQAAAKIGVQGQWIQVQDPKGDQGYVAGWFVSDKKAVPAPAAPV
                     TPASPSTSKPGTAPKPSSSASKPGSAPAPTPSKPAPVPPGALAFFPTEEVSFRSQPVV
                     ADNTLIRKIPVTEQLVSTEPADKVIPKVGVQNQWLKVRAADNKEGYVAAWYVKYAGGS
                     TAQANAAAAPAPAASTGGAIKVRTVAEAVSLRKQPVVSDATLIKRVPLGTEFTITEAG
                     GEAKIGKNDQWIKVKDASSEGYVAAWFVAR"
     gene            4759..6069
                     /locus_tag="PLT08_02100"
     CDS             4759..6069
                     /locus_tag="PLT08_02100"
                     /inference="COORDINATES: protein motif:HMM:NF033634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SLATT domain-containing protein"
                     /protein_id="HNE03282.1"
                     /translation="MENQTQEPNPILQIAWTRFAQMDAYSSRRTKNHLNFRRWLGILS
                     ILATLFAIIVSLFPSDNPSLIVVVFKFLLIMTPILSSGLAVYGSKFLSSGDWLVTRAG
                     AEEILKEIYTYRTVLKNSRTRDAWLENRLAEIQRSVFRGLNGELTIPPFEGYLPPHYN
                     QSDPYSDPGFSDLIGDEYFQYRLESQLGWHVRKVNKFQRERVRLQIFIILSGVFGSIL
                     AVILPVWTALAASFTAVFLGWQELRNLDSVVRNYSKVVMELTLLYDHWKNLKPGQKTP
                     SEFYKMVRSTEDILWSQNVEYIKAMQEALKESDLEEEASLVNRVIKEARESDRRLKQS
                     MEDAVVEMTTEKLHEAEEKLSETFEEALNTLAEEASSEIVQAELAAMREAIQEFAENA
                     WQGLSTSLKEIADEYKDIDVGTDTPRDVLNSLLARYPKTSEVKG"
     gene            6098..>7376
                     /locus_tag="PLT08_02105"
     CDS             6098..>7376
                     /locus_tag="PLT08_02105"
                     /inference="COORDINATES: protein motif:HMM:NF024677.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAFs-binding domain-containing protein"
                     /protein_id="HNE03283.1"
                     /translation="MTGSTETAQKAETTNTSATPAQAQPAVPGTENKGVPIIGPAGEP
                     AKNVDDSTVAYKEVDKSSLVVEVRPHAFIVMPFGKKKGGDGSLYDFNAIYKQLIKPAL
                     EEAGFEPFRADEETASGDILTDMFQELLLADLVLCDLSIDNANAFYELGIRHAFRKRG
                     VMHIQAGRAYMPFDIFNVRTLPYHVTPDGVPDAAFLKTDIQAIARMAKDTWASDRDAI
                     HSPIFNLLQGLKEPERKSLRTPLATGFWREYNEWKQRVTVAQRQKRIGDILLLTEEIS
                     NPLIKEEAIGEAGNALANMGRNELALAQYRKGLEVNSGNLDFRRKEAFHLNRLGRVDE
                     AIVKIESLLADFPNDSEAISYLGRIYKEMWADSWKQIPDKNKRLKAAFDAYHWLIKAI
                     DTYLKGYRIDLNNHYPGVNALTLSSIAIHLADKF"
BASE COUNT         1979 a         1847 c         1789 g         1761 t
ORIGIN      
        1 attagaacgg gatattgagc tacttgctta tcacggttct ccaaaaggga cgacgcattt
       61 agttctggca actacctcta cagaaacctt gaatgagtat tacagcgatc aaaccgcttc
      121 aatttttatt ggcggacact ctcacatcca aatggaacgc cgccatggca gtaagttatt
      181 tttgaatgca ggtagtgtgg gaaatgcgtt tcaattcgca tatactcccg gcaacccgcc
      241 caatctttta ccttgggcag aatatgctat tgtggagcaa gaaggaaatt tcttgcacgc
      301 cgatatgcgc cgtatctatt ttgacatcga tgccttactg gcgcgcgtaa aagaaagcaa
      361 attaccgggc gcgaattggt ggttcaaaca gtacgaaaac cgatagacat tttttgtttc
      421 tcacaatgcg gcatgatgcc gcattgttga ttcatagcat agacgaaagg caccgatatg
      481 accattcacg tcttgaaaaa tgcaacgttg atcaatggca ccggctcaaa acccatcgac
      541 cggggcttgg ttgccattga aggggaaacg attctcttcg ccggaaacat ggaaggctgg
      601 caaaatcctg aagggaaaat tatccatgaa atggatttaa ccggcaaaac cattttgccg
      661 ggtttgatcg acagccatgt gcatatcgct ttggaatgtt tgccagatag cactttgtcg
      721 ggtccgtggg gttggacgac gctcatcatg ctcaagcatg cgcagaatac cctcgcggcg
      781 ggcatcactt ccattcgaga tgtgggcgga aggcatcacc tcgaattctc tttgcggaaa
      841 gccattgaag caggtttatt tgttggacct agaatgtctc tcgctggaaa gttactctcc
      901 attaccacca cagggacgga atggtatgaa ggcatgtacc gtgaagcaga tggcgtggaa
      961 gaggtccgta aggcggcgcg agaacaactc aaagccgggg ctgacctgat caaagttttg
     1021 gcaactggcg ctgttctggc tccgggcgaa aaacccggtg ccgccacatt tgaattggaa
     1081 gaaattcgtg ctgccgtaat agaagccgca aaggtcggca agatcgtggc agcccatgcc
     1141 catggcattg aaggaattcg taatgctgtg gaagcaggtg caaagaccat tgaacatggc
     1201 acttatttgc atcaagacct gcgactaatt gaacggatgg cgaaagaaga aatttttgtg
     1261 gtgcctacgc tgaaagccgg ttttgacgta attgaaggag accgaccggg catccctgat
     1321 tggattttgg aaaaaagcaa agaaacacag gaagatgcga tgaaatccat tcgcaaggca
     1381 tatgaaatgg gagtacctat tgcgatgggt acggatgctg ccaccccata taattttcat
     1441 ggcgagaatg cgatggagct atattacatg tcgcaggcgg gaatctctgc aatggactgt
     1501 attgttgctt ctacgaagaa tgccgctcgc gcactcggct gggactcaaa gcttggaacg
     1561 ctggaagccg gtaaattagc cgatttgatc gtggtcaaaa agaatccgtt agatgacctg
     1621 cgttctttag ccaacagaaa taacattgaa tacgtcatgc aaggcggtaa atttgtggca
     1681 cgtcaattcg tagaaccgac cggcattcct gaagaattga tggctggcgc ttggatctgc
     1741 tgcggatttt agttttattg gaggttaaaa atgaaacgcg tttatcaata tctgtctgta
     1801 tatgtttttc tcgcacttgc ctgttccagc cttacaccga gtacatccac tggaacaggt
     1861 tatcgatacg ataatcatcc ggatgccaat acacagtcgg ctgaatatcg cgccctttcc
     1921 aaatggggca aaaccgatat cacctattat ttcatcaacg gcaccgacaa aatttcagga
     1981 gaccgagaac aagaattggt ggctcaggca tttgcactat gggcagcgca aacttcactc
     2041 actttcacag agaccaactc aagacaaagc gcggatattg tcattggctg ggcagaaggt
     2101 gaacacggag atggcgaccc atttgacgga cctggcgatg tactagcaca cgcctcttac
     2161 cccaacccgt atgaggagcg ccaagtcttt ttacattttg acgacgccga acgttgggtg
     2221 gatagcgata cccaagatgt agacctgctt accgtagcag cacatgagat cggtcatgcg
     2281 ttgggactgg gtcacagcaa cgacccgaac tctctgatgt atccatccta tagtgagccg
     2341 catcgttttc taggcgggga tgatattgcg ggcattcaat cactttatgg gttggcaagt
     2401 gaaccaccta ccgcaccaga agtgcccaag ccgaatgaaa acccgcccac cagcccaagc
     2461 caagactctg atggagatgg gctggcagac tcagatgaaa ctctcatcac aggcacagac
     2521 cccaacaaca aagattcaga tagtgatggc attggcgatg gcgtagaagt ggtcaaccgc
     2581 atgaatccgc ttgatgctga tatggataaa gacggagtga aagacggcgc ggaaattgcg
     2641 gcgggaacca atcctttcct gccagaccaa gccaatggag tctcgccgga gctttcgaag
     2701 gaagtcggcg aatttctcac gaaggcgatc aagctgcaaa ttgaagccta tcgccaaggc
     2761 gatgcttctg ttgcgtctgt gattatggct ggcgacgtgc tacagtctct acaaggagag
     2821 atttcctcgc tcaaccaaca ggggttggtc gagatcgcag acattgacta ttacaaaagc
     2881 tacatcgacg atattcgaat cgtcaacaat gctcagcttg aagtggatac ttgtgaagtc
     2941 tggtctttgc agtattatcg ccgttcagat ggtgcccttg ttcaaagcaa tgagcctacg
     3001 ctattgccac aaacgatcac tattcaaaag atagagaatg gctggtttat cactgcggtt
     3061 caattttttg aggcaccggc tttctgccat tcgtaaaatg cgccccattg gcaggtcgat
     3121 ttatgtggga aaaaactgcc aatggggtga gccaagaggc ggcggagaga gagccgggcg
     3181 gcatccacct taaaatattg caaaatcgtt gcaaaggcaa atcgagtaga attagataaa
     3241 tttatcctta aaaagcaaga ttgaaattcc caatctccaa atggaggagg caatttttat
     3301 gtttgttacg aagaacttgt accaaaatga tgaagcctgg aagaacgttc cgctcggttt
     3361 ttcatcgcag accatcggtt cttggggttg tttgctcact tcagtgacta tgatgttgaa
     3421 cggcatcggg tacaacgaaa cacccgatac ggtcaacact aagatgaaga acgcgggtgg
     3481 ttttcagggt ccgctcttca ttcccagtgt gctgccttac gtttggggaa actgcgcata
     3541 ccgcgatatg cagccttgtg aatctgttcc ggctccgctg caacagatcg acgatgcggt
     3601 tgcttctggc aaacccgtca ttttgcaagt ggactggaac aaacaagctg gtattcaaac
     3661 tcacttcgtc cttgtgaaag aaaagaaggg cgatgattat gtgctttatg acccgtataa
     3721 atacggcggc gacggtccag ataaagaagt attgctaacc acccgctaca aatacaacgg
     3781 tgccaaactc tccaccgaga ttagtgctgt attatggttc gaaaactacg gcatcaaccc
     3841 gcctgaacca cctgccaagg ttcccgttcc tgctgagaat ttcattctat atgccgccga
     3901 agatgatttg gctctgcgtt ctcaacccgc caccactggc tacctgtgga agcgcatggt
     3961 gatgaatact gagctcatct gccttgaacc caaagcccaa gccgccgcca agatcggcgt
     4021 gcaaggtcaa tggattcagg tacaagaccc gaaaggcgat cagggatatg tggctggctg
     4081 gttcgtttct gacaaaaagg ctgtgccagc tccggctgct cctgtaacgc ccgcctctcc
     4141 ttcgacgtcg aagccgggta cggctcccaa gccttcttct tccgcctcga aacccggttc
     4201 ggctcctgcc ccaaccccaa gcaaacccgc gcccgttcct cccggcgcct tggcattctt
     4261 ccccaccgaa gaagtctcct tccgatctca gcctgtcgtt gcagataaca ccttgatccg
     4321 caagatccca gtgaccgaac aactcgtcag cacagaacct gccgataagg tcattccgaa
     4381 agtcggcgtg caaaatcaat ggctcaaggt gcgtgccgct gataacaaag aagggtatgt
     4441 agcagcttgg tacgtgaagt atgccggtgg ttccacagct caggcaaatg ctgcagccgc
     4501 tcctgcccct gccgcttcaa ctggtggcgc gattaaagtc cgcaccgtgg cagaagccgt
     4561 ctctttgcgc aaacagcccg ttgtcagcga tgcgactctc atcaagcgtg ttccgctcgg
     4621 cacagagttc accatcaccg aagctggtgg cgaagccaaa attggcaaga acgatcagtg
     4681 gatcaaggtc aaggatgcga gcagtgaagg ttatgttgca gcctggttcg tagctcgtta
     4741 ggcataaaga ggatactcat ggaaaaccaa actcaagaac ccaatcccat cctccagatt
     4801 gcgtggaccc gttttgcgca gatggatgcc tattcgtcgc gtcgcaccaa gaatcactta
     4861 aacttccgcc gttggctcgg cattctcagt attctggcga ccttgttcgc gatcattgtt
     4921 tcgctattcc cttcagacaa cccatcgcta attgttgtgg tcttcaaatt cctgttgatt
     4981 atgaccccca tcctttcttc cgggttagcg gtctatggaa gcaaattcct ctccagtgga
     5041 gattggctgg ttacacgtgc tggtgctgaa gaaattctca aagaaatcta tacctatcgc
     5101 accgttctaa aaaattcacg cactcgcgat gcctggttgg aaaatcgctt ggctgaaatt
     5161 caacgttcgg tctttcgcgg tttgaacggc gaattgacca tcccgccctt tgaaggctat
     5221 ttaccgcctc actacaacca atcagacccc tatagcgacc ccggttttag cgacctaata
     5281 ggcgatgaat attttcaata tcgccttgaa tctcagcttg gttggcatgt cagaaaagtc
     5341 aacaagttcc agagagaacg tgtgcgctta caaatcttta tcatcctctc cggtgttttc
     5401 ggctccattt tggctgtcat tctcccagtt tggacggcac tcgccgcttc gttcacggct
     5461 gttttcctcg gctggcagga acttcgtaat ctagactcag ttgtccgcaa ttacagcaag
     5521 gttgtcatgg agttaactct cctctacgat cattggaaga acctcaaacc cggtcaaaag
     5581 actccttcag aattctacaa gatggttcgc tctacagaag atatcctctg gagccagaac
     5641 gtggaataca ttaaagccat gcaagaggcg ctcaaagagt ctgacctcga agaagaagcc
     5701 agccttgtca accgcgttat taaagaagca agagaatccg accgccgctt gaaacagtct
     5761 atggaagatg cagtggtaga aatgaccact gaaaagctac atgaagctga agaaaagtta
     5821 agcgaaacct tcgaagaagc tttgaataca ttggcagaag aagcctcttc tgagatcgtt
     5881 caagcagagc ttgctgcaat gcgcgaagct attcaagaat ttgctgaaaa cgcatggcag
     5941 ggactttcta cctccttgaa agaaattgca gacgagtaca aggatatcga tgtcggtacc
     6001 gatactccgc gtgatgttct caacagcttg cttgcccgtt acccgaagac cagcgaagtc
     6061 aagggataga gtcatatccg cttcatagga gattacaatg accggctcaa cagaaactgc
     6121 acagaaagct gaaacgacca atacctcagc aaccccggca caagcacaac ctgccgtgcc
     6181 tggtactgaa aataaaggtg tccccattat tggtcccgct ggagaacctg cgaaaaacgt
     6241 agacgactct accgtggcat ataaagaagt ggacaaatcc agcctcgttg ttgaagtgcg
     6301 tccgcatgca ttcattgtta tgccttttgg aaagaaaaaa ggcggcgatg gatctcttta
     6361 tgactttaac gccatttaca aacagctgat taagcctgcg ttggaagaag ccggcttcga
     6421 acctttccgt gccgatgaag aaaccgcctc gggcgacatc ctcaccgata tgttccaaga
     6481 gttgctgctt gccgatctag tgctgtgtga cttaagtatt gataatgcca atgcttttta
     6541 tgaactaggc atccgccatg cgttccgcaa acgcggcgtg atgcacattc aagctggtcg
     6601 cgcctacatg ccgttcgata tcttcaatgt gcgcaccctt ccctatcatg tcacccccga
     6661 tggcgtgccc gacgccgcat tcctcaagac agacattcaa gccattgccc gtatggcaaa
     6721 agacacctgg gcttctgacc gagatgcaat tcacagccca attttcaact tgctccaagg
     6781 gctgaaagaa ccggaacgaa agtcgcttcg cactccgctg gcaactggat tctggcgcga
     6841 atacaacgaa tggaaacaac gtgtaaccgt cgcacagcgc caaaaacgca tcggcgacat
     6901 ccttttactc acagaagaga tcagcaaccc gctaatcaag gaagaggcga ttggtgaagc
     6961 tggcaatgcc cttgcaaaca tgggacgcaa cgagttagca cttgcccagt atcgcaaggg
     7021 cttggaagtc aactctggca atctcgattt ccgtcgtaaa gaagctttcc acctcaaccg
     7081 ccttggtcgt gtggatgaag ccattgtcaa gatcgaaagt ctgcttgccg attttccaaa
     7141 tgatagcgaa gccatctcat accttgggcg catctataaa gaaatgtggg cagattcttg
     7201 gaaacaaatt cccgataaga acaaacgcct taaagccgct ttcgacgcct atcattggct
     7261 tatcaaagcg atcgatacct atctcaaagg ctatcgtatc gacttaaata accactaccc
     7321 tggcgtcaat gcattgactc tttcttcaat cgccattcac ctcgctgaca agttcg
//
LOCUS       DAOQJE010000046         7365 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_5557_length_7365_cov_6.256635, whole genome shotgun sequence.
ACCESSION   DAOQJE010000046 DAOQJE010000000
VERSION     DAOQJE010000046.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 7365)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7365)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7365
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5557_length_7365_cov_6.256635"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..982)
                     /locus_tag="PLT08_02110"
     CDS             complement(<1..982)
                     /locus_tag="PLT08_02110"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HNE03284.1"
                     /translation="MSNDIIHILLVEDEDPHAELIQRAFEDQGSEFQIHRVRSLTEAR
                     EHRRLNEPKLIIADWRLPDGESLELLPNHRDPLATPIILMTSYGNERIAVEALKSGAL
                     DYVVKSPESMLDMPHLARKALDQWKSRADRIHMQEALTESEAHFRLLAENASDMITRI
                     NTNGQMFYISPACKTILGYQPEEMLGVKVFDLINPEDQPQIRELFSQKPEDSPYTITY
                     RAKHKSGHYVWLETSARAIIDHSTDEVIEIQAASRDITERKRAEEALQLAHNQLRDAY
                     ERTIEGWVRALDLRDRETEGHTQRVTDITIRVAQQLGFSEEELSHIKRGAL"
     gene            complement(989..1432)
                     /locus_tag="PLT08_02115"
     CDS             complement(989..1432)
                     /locus_tag="PLT08_02115"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNE03285.1"
                     /translation="MLGEPILVMLVEDNADHAELVIRTMESHHIPTRIQHVTDGESAL
                     DYLLRRNNFSDPETSPRPHMILLDLRLPRVDGLEVLRIIKEEEDLKSIPVVILTTSEA
                     EKDVMHAYEHYVNSYLVKPVGFEDFSSLMKDLGIYWLGWNKQLRA"
     gene            1605..2744
                     /locus_tag="PLT08_02120"
     CDS             1605..2744
                     /locus_tag="PLT08_02120"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HNE03286.1"
                     /translation="MTESALLNDRYQLDELLGSGGMANVYRARDVVLDRPVAIKVLRK
                     EYSTNENFQKQFWLEARSAANLSHPNIVTVHDFGAADDLLYIVMELVPGKDLKQLVRE
                     RGRFPYQQGIPLVIQACAGVGYAHRAGLVHCDIKPHNMLVSKDMRLKVTDFGIARALA
                     TIKPGERNDVVWGSPLYFAPEQAAGEAPTPASDVYSLGVVLYEILTGTTPFTASTADE
                     LARLHISARPLPISEYVPDVPPALEEIVMKVLSKEPAARYRTADQLGRVLQKFGTEPA
                     APPKPKQEPVSSPPVITIKQDIAERLSPPSQPRPQPEPAPQYYQPQYPPQSIQPEPAP
                     TYREEEVYTEPIDWLAVGLGLMAVLAWGGLIPFGLMIYLSYFPPS"
     gene            2792..3457
                     /gene="rpe"
                     /locus_tag="PLT08_02125"
     CDS             2792..3457
                     /gene="rpe"
                     /locus_tag="PLT08_02125"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HNE03287.1"
                     /translation="MTHYLLAPSIIASDFAHIADEVAAIESAGADWIHVDVMDGHFVP
                     DITIGPLFVKGLRRVAKLPLDAHLMISEPEKYIPAFAEAGVNNITVHVETCPNLPKVI
                     EQIKSHGCTAGVTLNPATPASAIEAVLPLVDLVLVMSVVPGFSGQKFMPEMINKVAEI
                     RAKLNVLGSHAHLEVDGGINAETLPLMKNAGANVFVTGNAAFKHPQGSGAGVKALKEI
                     LNK"
     gene            complement(3543..3728)
                     /gene="rpmB"
                     /locus_tag="PLT08_02130"
     CDS             complement(3543..3728)
                     /gene="rpmB"
                     /locus_tag="PLT08_02130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HNE03288.1"
                     /translation="MAKCNHCGKATTFGHNRSFSQRATNRTFKPNLQKVSVMEKGRLV
                     QKTLCTKCIKTLGKSAA"
     gene            3951..4307
                     /locus_tag="PLT08_02135"
     CDS             3951..4307
                     /locus_tag="PLT08_02135"
                     /inference="COORDINATES: protein motif:HMM:NF015721.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HNE03289.1"
                     /translation="MGEETTSLGGIHVSPNAVATIAYHATLQSYGVVGLAPKNLADGI
                     VSTITREPSRGISVKYLEDEIHIDINVVVEYGTRINSVAESVANTVRFHVEKALGLKV
                     NTVNVHVAGLRISNTD"
     gene            4322..5989
                     /locus_tag="PLT08_02140"
     CDS             4322..5989
                     /locus_tag="PLT08_02140"
                     /inference="COORDINATES: protein motif:HMM:TIGR03599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DAK2 domain-containing protein"
                     /protein_id="HNE03290.1"
                     /translation="MAIDTARVEKFRKRTIDGQAFKRLVEAGMTWLRTNQQVVNSLNV
                     FPVPDGDTGTNMSLTLQAAWNEVKDKGTKHLGEMAAAFSKGALMGARGNSGVITSQIL
                     RGFSRGVHEVETLDVETFIKALAEARDTAYKGVVKPVEGTILTVIKETANAAEAARGS
                     VKDAIELLELAVKAADEAVKKTPEMLPILKQAGVVDSGGKGLFFFMEGMLRHIYGESL
                     ETPTMQVHALSAVGLEDALEVVEEGQDYEVVVDFVPNGELDLHGFYAKLEEMGTSIQV
                     GEGDGMYRMHIHVPTENRYVPIDYIMGIGTVTKVAIENLMAQMDDIQKSKSSQIQFST
                     VEPGNIAVVVVSPGTGISRIFASLGVAAVVTGGQTMNPSTQDILSSFENMPTDKVIIL
                     PNNKNIVMAANQAKDVTVKSVQVVPTRTVPQGLAAMLSFDPSGDVEAVAAKMNKAMGN
                     VKTGEITVATRSVEIDGVTVKDGQVIALLDGKLVASSESVEQGVFDLLEKAEASEHEI
                     VTLFYGADLPHAEANRVADSIREKYSNLEVEVQEGGQSHYQFILSIE"
     gene            complement(6045..7064)
                     /locus_tag="PLT08_02145"
     CDS             complement(6045..7064)
                     /locus_tag="PLT08_02145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE03291.1"
                     /translation="MNLIDRYVSEVGKHLPLLNGREDIEKELKSTLEDMLEDRAQSTG
                     RTRDEALEIEVLKEYGSPQKIASTYNPHPYLIGPKLFPFFLFVLKIVLTVVVSVMLVL
                     AGVQAVTDTPFMGADFVKIIGQGLGSALSAAIAAFGNVVLVFVIIERVLPDKEIGGFN
                     DEKDWDPASLTKEPDPDSVSRFELITEIVFTFVGLAILNIYPQWLGMFFVTGEETVFI
                     SLFSEKFFQFVPWINLTLVAQILLDVYLLRNALWNRLSRLFKVFIEVASLSLTAIILR
                     TPGIIGFNAESLLNGPFPPEQAKVFVTIATYTFPIVMAVVIIIQSIELIKSIYKLFKS
                     NRQSS"
     gene            complement(7080..>7365)
                     /locus_tag="PLT08_02150"
     CDS             complement(7080..>7365)
                     /locus_tag="PLT08_02150"
                     /inference="COORDINATES: protein motif:HMM:NF015513.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HNE03292.1"
                     /translation="RGVIVLAALSQLGSPEYGYSLLKKLADLGLEVDQGTLYPLLRRL
                     ETQGLLESVWKLEEARPRRYYVISAEGKKLLPKMKKEWAGIVSVMDKMLS"
BASE COUNT         1881 a         1728 c         1854 g         1902 t
ORIGIN      
        1 gcaatgcgcc tcgtttaata tgggagagtt cttcttcgct gaacccaagc tgttgtgcaa
       61 cccgaatcgt aatatcggta acgcgttgtg tgtgaccttc tgtttcgcgg tcacgcaagt
      121 ctaatgcgcg gacccaacct tcaatggtgc gttcgtacgc atctctgagt tggttgtgtg
      181 cgagctgaag tgcttcttct gctcgcttac gttctgtaat gtcacgtgat gcagcttgaa
      241 tttcaatgac ttcatctgtg ctgtgatcga tgatggcacg tgcggaggtt tccaaccaaa
      301 cgtaatgtcc gcttttgtgt ttggcacgat aggtgatggt gtatggggaa tcttcaggtt
      361 tttgagaaaa aagctctctg atctgaggct gatcttcggg gttaataagg tcaaatactt
      421 tgacccctaa catttcttca ggctgatatc caagaatggt tttacaagcc ggtgaaatat
      481 aaaacatttg cccgtttgta ttgatgcgag tgatcatgtc actggcgttc tcagccagta
      541 agcgaaagtg agcttcactc tctgtaaggg cttcctgcat gtgaatgcgg tctgcccgag
      601 acttccattg gtccagtgct tttcgtgcca agtgaggcat atccagcatt gattcagggg
      661 acttcaccac ataatctaac gcaccagact ttaacgcctc taccgcgatg cgttcattgc
      721 cataacttgt cattagaatg atgggggtag ccagcgggtc gcggtgattg gggagcaatt
      781 ccaagctttc cccatctggg agccgccaat ctgcgatgat aagtttgggt tcgttcaagc
      841 gtctatgttc gcgggcttct gtaagagaac gcacacggtg aatctgaaac tcagacccct
      901 ggtcttcaaa agcgcgctga atcagctccg catgcggatc ttcgtcttca actaaaagaa
      961 tatgaatgat gtcattcgac atgagggatt aagcgcgtag ttgtttattc caacccaacc
     1021 agtaaatgcc aaggtctttc attaagctgc tgaaatcttc aaacccaacc ggctttacta
     1081 gatagctgtt gacataatgt tcatacgcgt gcataacgtc tttttcagct tcagacgttg
     1141 ttagaataac aacaggaatg ctcttaaggt cttcttcttc tttgatgatt cgcagtactt
     1201 ccaacccatc aacacggggt aagcgtaagt ccaaaaggat catatgtggg cgagggctgg
     1261 tttcggggtc actaaagttg ttgcgacgca ggaggtaatc caatgctgat tcgccatcgg
     1321 ttacatgttg aatccgtgta ggaatgtgat gactctccat ggttcggatg acaagttctg
     1381 catggtcagc attatcttca acgagcatga caaggattgg ttcgcctagc atggtggatc
     1441 tcctaaacag tcatattgta aacgatttac gaataacggg tgaaaatcgt tatgacaact
     1501 ttatttaaca gacctgttaa gacaactaaa catataaact ccccaaaata tcgattttct
     1561 atctccgtgc ctctatgtag aactcacggt ataatctcac cacaatgacc gaaagtgcat
     1621 tactaaacga ccgatatcaa cttgacgaac ttctcggctc tggcggcatg gcgaacgtgt
     1681 accgcgcgcg cgatgtggtg ctagaccgtc ctgttgcgat taaggtctta cgcaaagaat
     1741 attccaccaa cgagaatttt caaaaacaat tctggctgga agcgcgttcc gcggcgaatc
     1801 tttcgcaccc caatattgtc accgttcatg attttggcgc tgccgatgat ttgctctaca
     1861 tcgtcatgga acttgttcct ggcaaagacc tcaagcaact cgtacgagag cgcggacgtt
     1921 tcccttatca acaggggatt ccgcttgtta tccaagcttg tgctggggta gggtatgcgc
     1981 accgcgctgg cttggttcac tgcgatatca agccgcataa tatgcttgtt tccaaagata
     2041 tgcgcctcaa agtcacagat tttggtatcg cacgtgcgtt ggcaaccatc aaacctggtg
     2101 aacgaaacga tgtcgtatgg ggctctccgc tctactttgc gcccgagcaa gccgcaggcg
     2161 aggcgccaac cccagcttca gacgtgtact cgttgggcgt tgtgctctat gaaattctga
     2221 caggaaccac ccctttcacc gcctccactg ccgatgaact ggcacgtttg cacatctccg
     2281 cacgtccgct gcctattagc gaatacgtgc cagatgttcc gcccgccctt gaagaaattg
     2341 tcatgaaggt actttcaaag gaaccggcgg cacgatatcg cacagcagat caactggggc
     2401 gcgtcctcca aaaatttgga accgaacccg cggctccgcc taagccgaag caggaacctg
     2461 tttcctcgcc gcctgtaatt accatcaaac aggatattgc cgaacgactt tcacctccga
     2521 gtcaacctcg accgcagcct gaaccggctc cgcaatatta tcaaccgcaa tacccgcctc
     2581 aatcgattca gccggagcct gctcctacct atcgtgaaga agaggtttac accgaaccta
     2641 ttgactggct ggcggttggg cttgggctca tggctgtcct cgcatggggc ggactcattc
     2701 cctttgggtt gatgatctac ctctcgtact tcccaccaag ttaaatggac agacctgaca
     2761 gttcacctaa actcgtcagg tctttttgat aatgactcac tatcttttag ccccttctat
     2821 tattgcttct gattttgcgc acatcgcaga cgaggttgct gcgattgaat ctgccggtgc
     2881 cgactggatt catgtcgacg tcatggacgg acactttgtc cccgatatca ccatcggacc
     2941 gttattcgtc aaagggttga ggcgagttgc caagcttccg ctagatgccc atctcatgat
     3001 ctctgaaccg gagaaataca tccctgcatt tgctgaagca ggcgtcaata acatcactgt
     3061 ccatgtagag acttgcccca atttgcccaa agtgatcgaa cagatcaagt ctcacggctg
     3121 caccgccggt gtgaccttga acccagcaac ccctgcttct gccattgagg cggttcttcc
     3181 tcttgtggac ttggtattag taatgagtgt cgtcccgggt ttttcagggc agaagtttat
     3241 gcctgagatg atcaacaaag tggcagagat tcgagccaag ttgaacgtac ttggttcaca
     3301 cgctcacctc gaagtggatg gcggtattaa tgcagagact cttccgctca tgaaaaacgc
     3361 cggagccaat gtttttgtaa caggcaatgc tgcattcaaa cacccacaag gtagtggggc
     3421 aggggtaaag gcattgaaag aaatattaaa caagtagggc agggtcaacc tgccttttca
     3481 gtgttaaaac aaaaatcacg gcgcttacgc gccgcgattc ttgagtcaaa aggaacgaaa
     3541 ccttatgccg ctgatttgcc aagcgttttg atgcacttgg tgcacagcgt cttctgcacg
     3601 aggcgaccct tttccatcac cgaaaccttc tggaggttgg gcttgaacgt gcggttggtg
     3661 gcacgctgag aaaaggagcg gttgtgaccg aaggtagttg ccttgccgca gtgattgcac
     3721 tttgccatga ttgaaatcct ttctttctcg tctttccttg caaataattc caaatgccct
     3781 tttcgaaggc acggcatttt accatagaac gcaaccaact tcaagataag atgttaaaat
     3841 aggaacagga ttataaaaac ggggcggcgg agcacggtgt gccgttctcg agtgtatgtg
     3901 aacaacgaaa atcagacgtt taggtctgaa tattaaggaa tgaggaacct atgggcgaag
     3961 aaacaacatc cttgggcggg atccatgtct caccgaatgc ggtggctacg attgcgtacc
     4021 atgccacctt gcagtcttat ggtgtggttg ggctggctcc gaagaattta gccgatggta
     4081 tcgtctccac gattactcgc gaaccttcgc gcggtatttc ggtgaaatat cttgaagacg
     4141 agatccatat tgatatcaat gtggttgtgg aatacggcac gcgcattaat tcagtggctg
     4201 aaagtgtggc gaatacggtt cgttttcatg tagaaaaagc gttaggtctt aaagtaaata
     4261 cggtcaatgt gcatgtagcg ggattgcgca ttagcaatac ggactaggag aaaagcgata
     4321 tatggcgatc gataccgcgc gagttgagaa attccgcaaa cgtaccattg atggacaggc
     4381 tttcaagcgc ctcgtagaag ccgggatgac ctggttgcgt accaatcaac aagtggtcaa
     4441 ctcattgaat gtgtttcccg ttcccgatgg ggatacaggc acgaatatga gcctcacgtt
     4501 gcaggcggct tggaatgaag ttaaggataa aggcacgaaa catttagggg agatggccgc
     4561 tgcattttca aagggtgcgt tgatgggcgc gcgcggcaat tcgggcgtca tcaccagcca
     4621 gatcctacgc ggattttcac gcggcgtaca cgaggttgag accttagatg ttgaaacctt
     4681 tatcaaggct ttggcagaag ctcgtgatac ggcgtataaa ggggtcgtca agccggtgga
     4741 agggacgatt ctcaccgtca ttaaagaaac ggcaaatgcg gcagaagcag ctcgcgggtc
     4801 cgttaaagat gcgattgaat tgctcgagct tgccgtcaaa gccgcagatg aagcggtcaa
     4861 gaaaacgcct gaaatgcttc ccatcctcaa gcaagcgggt gttgtggact cgggtggtaa
     4921 aggcttgttc ttcttcatgg aagggatgtt gcgccatatt tatggtgaat ctttagagac
     4981 gcccaccatg caggtgcatg cgctttctgc ggttggattg gaagatgcgc tcgaagtggt
     5041 agaagaggga caagattacg aagtggtggt ggacttcgta ccaaacggtg aattggactt
     5101 gcatggtttt tatgccaagc ttgaagagat gggcacctcc attcaagtgg gtgagggtga
     5161 tggaatgtat cgtatgcaca ttcacgtgcc gactgaaaat cgctatgtgc caattgatta
     5221 catcatgggc attggcactg tgaccaaagt tgccattgaa aacttgatgg ctcaaatgga
     5281 tgatattcaa aagagcaaat cttcacagat tcaatttagc acggtggagc caggcaatat
     5341 tgcggtggtg gttgtttcgc cgggcacagg tattagccgt atctttgcaa gtttgggtgt
     5401 tgcggcagtg gtcaccggcg gacagacgat gaacccctct acacaggata ttctcagctc
     5461 gttcgagaat atgcccacag ataaagttat tattttgccg aacaataaga atattgtgat
     5521 ggcggcaaac caagccaagg atgtgacggt taagagcgtg caggtggttc cgacccgcac
     5581 ggttccgcag ggacttgcgg cgatgctttc ttttgacccc agcggtgatg tggaagcggt
     5641 tgcggcgaag atgaataagg cgatgggcaa cgtcaaaacc ggcgaaatca cggttgcgac
     5701 tcgttctgtg gaaattgatg gtgtgaccgt gaaagatggt caggtgattg cgctcttaga
     5761 cggcaaattg gttgcctctt ctgagtctgt tgagcaaggt gtgtttgact tgcttgagaa
     5821 ggcagaagcc agtgagcatg aaattgtcac tttgttctac ggtgccgatt taccccatgc
     5881 agaagccaat cgtgtcgcag actccatccg cgagaaatac tccaacctcg aagttgaggt
     5941 gcaggagggt ggtcagtctc actaccagtt cattctttcg attgaataat tgggaaacca
     6001 agttaagcaa agaggctgtc acttatgtga cagcctcttt ttatttagct gctttgacgg
     6061 ttcgatttga atagcttata aatagatttg atcaattcaa tactttgaat gatgataacg
     6121 actgccatca cgatggggaa ggtgtaggtt gcgatcgtta caaagacttt tgcctgctca
     6181 ggtgggaaag gtccgttcaa aagagattca gcgttgaacc cgatgatccc cggtgtgcgt
     6241 aggatgattg cggtcagcga gaggctggca acttcaataa acactttgaa cagacgagat
     6301 aagcggttcc aaagggcatt tcgcaataga taaacatcca gcaaaatttg agcgaccaat
     6361 gtaaggttga tccacggtac gaattggaag aacttctctg agaacaggga gataaagacg
     6421 gtctcttctc cggtaacgaa gaacataccg agccattgcg ggtagatatt caagattgca
     6481 agcccaacaa aggtgaagac gatttccgta atcagctcga aacggctcac agaatcagga
     6541 tcaggctcct tggtgagtga ggcggggtcc caatcctttt cgtcattgaa gccaccaatt
     6601 tctttatccg gcaagacgcg ttcaataatg acgaagacca aaactacatt tccaaacgca
     6661 gcgattgctg cagagagggc actgcccaat ccctgcccga tgattttcac aaaatcggcg
     6721 cccatgaaag gcgtatcggt cacagcttga acgcctgcca acaccagcat cacactgaca
     6781 actactgtaa ggacgatctt caatacaaag agaaaaaacg gaaacagttt tggaccgatc
     6841 aaatatggat gtgggttata ggtggatgca attttttgcg gcgagccgta ttctttcaag
     6901 acttcgattt cgagtgcttc atcacgagtg cgcccggtac tttgagcgcg gtcttccaac
     6961 atatcttcca gcgtggactt taactctttt tcaatgtctt cacgcccatt caataacggc
     7021 aaatgcttgc caacttcaga aacataacga tcaataagat tcatgattct tccctttctt
     7081 tatgagagca tcttgtccat gacagagaca atgccagccc attctttttt catctttggc
     7141 agaagttttt tgccttctgc actgatcaca taataacgac gtggacgcgc ttcttccaac
     7201 ttccaaactg actcaagcag accctgtgtt tccaaacggc ggaggagggg atacaacgta
     7261 ccctgatcca cttccaagcc gaggtctgcg agtttcttca gcagtgaata tccgtactct
     7321 ggcgagccta attgactaag cgccgccaat acgatcaccc cgcgc
//
LOCUS       DAOQJE010000047         7362 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_5561_length_7362_cov_6.138771, whole genome shotgun sequence.
ACCESSION   DAOQJE010000047 DAOQJE010000000
VERSION     DAOQJE010000047.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 7362)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7362)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7362
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5561_length_7362_cov_6.138771"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(119..583)
                     /locus_tag="PLT08_02155"
     CDS             complement(119..583)
                     /locus_tag="PLT08_02155"
                     /inference="COORDINATES: protein motif:HMM:NF016060.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HNE03293.1"
                     /translation="MILTDSRERTRFFKFAFVGAIGAAIDFGVMNLLSHVILELPLVF
                     AGSISFICAVISNFTWNRYWTYPESRSRPLLSQLGMFFAVNTAGMLIRIPILHYLEPP
                     LLQFVEDSFHTTYLTAEFYAKNLTLATAVGIVMLWNYFVNRYWTYNDIDDDN"
     gene            complement(580..1539)
                     /locus_tag="PLT08_02160"
     CDS             complement(580..1539)
                     /locus_tag="PLT08_02160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HNE03294.1"
                     /translation="MRILITGAAGFLGSHLCDRLLADGHEIIGMDNFITGNPDNIAHL
                     AGNEKFQFFKRDVSNYIFVPGKVDAILHFASPASPNPQSKSGYFNLPIQTMKAGALGT
                     HNCLGIARREGSRFLLASTSEIYGDPEIHPQAETYPGNVDPVGERAVYDEAKRFAESM
                     TMAYHRFHSVNTSIVRIFNTYGPRMDLEDGRALPNLLKQALLGEPLTVYGTGSQTRSF
                     CFVDDLVDGIVKLLYSKEHLPVNIGNPVEMSILEFAEIINKVTGNTAGIKYIENARSA
                     RDPQRRRPDITRAKDILNWEPKVNLEEGIRRTIPFFKQKLGLA"
     gene            complement(1563..3152)
                     /locus_tag="PLT08_02165"
     CDS             complement(1563..3152)
                     /locus_tag="PLT08_02165"
                     /inference="COORDINATES: protein motif:HMM:NF019142.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="G5 domain-containing protein"
                     /protein_id="HNE03295.1"
                     /translation="MPRLTLRPSLLATILLLVTLLASCKSPQLNSDITITVTADGTTR
                     DITVPQGQTVSQALESAGITVNSLDKTEPPLYTVIGNGDLVTLIRVEEVFETEEIVVP
                     FERQVVRNETLPEGETRLVQAGVNGLEEITYRKILEDKVETSQSIVKTTVIKEALPEI
                     VMVGAQTSFVPLNIPGTLAYLAGGNAWIMEGSTANRRVIVSTGDLDGRIFTLSPNGEY
                     LVFTRKSSKPADEEINTLWAVRTRNLDPKLISLQAKNVVHFAAWIPGTNSIAYSTVEP
                     RSAAPGWQANNDLYRVSITNSGSPKRILYPSSGGVYGWWGMNFAFAPDGRLAYSRPDG
                     IGLVDQDSGRLKPLLDITPLNTHSDWAWIPPLVWGADGNGLYFVSHAPAPAPISGEES
                     PYFDLSAISFKNEAAVSLVNSAGMFSYPAISPIQYGSREAAFQIAYLQSIFIDQSDSS
                     RYRLVVMDRDGSNSRALFPPADKNGLEPQTPVWAPAAVEGQSGEFIAVMYQGNLWLID
                     SGNSNTYQVTGDGLITRLDWK"
     gene            complement(3172..3741)
                     /locus_tag="PLT08_02170"
     CDS             complement(3172..3741)
                     /locus_tag="PLT08_02170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE03296.1"
                     /translation="MKTKNYIYLFIVSLVSASLACSINVGGPEYPAQPISASPTDAQA
                     LRDMIQQAMVTGAETGVITLSVTESQLTGYMAEKLSQQTNPPFTDPQILLRNGQMQMY
                     GKVTQGWFTANMLITMNVTIDETTGQPKIQITSADFGPIPAPEGVNNAISAIIEEAFT
                     GSFGPVAVGFRLQTITIADGIMTLTGRIK"
     gene            3786..4355
                     /gene="scpB"
                     /locus_tag="PLT08_02175"
     CDS             3786..4355
                     /gene="scpB"
                     /locus_tag="PLT08_02175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HNE03297.1"
                     /translation="MSESEPITNEELSLAAKIEAMLFVSADPVPVQQLSQALDVTASV
                     VERGLKELDEALKSRGLRLQRNAGRVQLTTAPEIAGLVETFLGLEAITHLSRAALETL
                     AIIAYQQPVTRPQIDSIRGVNSDGMMKSLLSKGLIQESGRTEGPGRPILYSTTPEFLQ
                     HFGLSSIMELPPLATPIETEPEPQELLKG"
     gene            4370..5125
                     /locus_tag="PLT08_02180"
     CDS             4370..5125
                     /locus_tag="PLT08_02180"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HNE03298.1"
                     /translation="MQKILAQAGYGSRRACEEFIIEGRVSVNGQIAILGQKADLAVDT
                     IKVDGKSLSKPETMTYIALYKPRNVLSAAEGQDDRKTVRDLIPAPGHLYPVGRLDFDS
                     EGLILMTNDGDLTNKLTHPRYAHEKEYRVLLARKPDTEQLDAWRRGVVLPEGDRTQPA
                     NVHFISTSGKGAWIRVVMGEGKKRQIREVGKLLGLPVVRIIRVRIGTLKLGDLKPRQW
                     RYLTEDEIRELKGDKVPKMEQKPRPKRTTTRRA"
     gene            5148..6476
                     /locus_tag="PLT08_02185"
     CDS             5148..6476
                     /locus_tag="PLT08_02185"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase"
                     /protein_id="HNE03299.1"
                     /translation="MKRLNFIVIIALVASTLGACQVLPRINDTRPNFLIIVTDDQRYD
                     TMEYMPNTQELIFDQGVTFSKGYITTPFCCPSRASILTGLYAHNHWVQVNEDPLEFRT
                     MVEDIHRNGYYTGLVGKYLNSWKGDMRPEYDFWVSFWGGTMKNYYDPDLNVNGTWSKH
                     TGYSTYLFRDFGVQFIEEASKQSKPFLLMYTPNAPHAPFTPAEEDVSLLTDLAPFRPL
                     SFNEEDVSDKPDSISNMAPLSEEDIKSIDNTRRRQLLTLMALDRTIPDLINKLKETGE
                     LDNTVIIFISDNGKHWGEHRLDSKSTAYEESVKVPFAIRYPPLIPTPYVDNRIVANID
                     IAPTIYELSETRLPDLMDGTSLVSLFSGETEWRDHILLEAWPDRGAWSAIHTGDYVYI
                     ETQGDLSEFYDLTLDPFEMDNAINDPQYAEMIAQLQQYLQTEKQPKTSSP"
     regulatory      6537..6640
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00504"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="glycine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="glycine"
                     /db_xref="RFAM:RF00504"
     gene            6880..7257
                     /gene="gcvH"
                     /locus_tag="PLT08_02190"
     CDS             6880..7257
                     /gene="gcvH"
                     /locus_tag="PLT08_02190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HNE03300.1"
                     /translation="MNNPTNLKYAKSDEWFDPSTGAVGISDYAQGQLSDIVFVEILVD
                     EGEEVTVGKAIASVESVKASSEIYAPVAGKVTAVNKDLSNTPETINTDPYGAAWMIKI
                     DGSASADLMDAAAYETHCAGRSH"
BASE COUNT         1957 a         1672 c         1836 g         1897 t
ORIGIN      
        1 acgaagccga tccaatctcc tgttcgatta aagagataag gatataaaag gaacgcatct
       61 gcgtcccctt tagtgctgta gattggaata aatgatactt gtgccataac gtcctgcctt
      121 aattgtcgtc atcaatatca ttatacgtcc agtaccggtt gacgaaatag ttccataaca
      181 tcacaatgcc cactgctgtc gcaagagtca aatttttggc atagaactcg gctgtaagat
      241 aggtcgtgtg aaaagagtct tcgacaaatt gcagcaacgg cggttcgaga taatgaagga
      301 tgggaatacg aatcagcata ccagccgtgt tgactgcaaa gaacattccc aactggctca
      361 gaagcggacg ggaacgcgat tcgggatacg tccaataacg attccaagtg aagttactga
      421 tcactgcaca gatgaaagag atgctcccgg caaataccaa cggcaattca agaatgacgt
      481 gtgaaagcag attcatcacg ccgaaatcga tggcggcgcc aatggcacct acgaacgcga
      541 atttgaaaaa gcgcgttcgt tctcttgagt ctgtcaggat catgccagcc ccaatttttg
      601 tttgaagaat ggaatcgtgc gccggatgcc ttcctctaaa ttgactttcg gttcccaatt
      661 caagatgtct tttgcacggg tgatatcagg gcggcgacgt tgtgggtcgc gagcactacg
      721 ggcattttca atgtacttaa taccagctgt gttgccggtg actttgttta tgatctcggc
      781 aaattccaaa atggacattt ctaccgggtt gccaatgttg accggtaaat gttccttgga
      841 atacaacaac tttacgatgc cgtccaccaa atcgtcaacg aagcagaaag aacgggtttg
      901 gctgccagtt ccatacacgg taagaggctc tcctaacaat gcctgtttca gcaagttagg
      961 caaggcacgc ccatcttcca aatccatgcg tggaccgtac gtattaaaga tgcgcacaat
     1021 gctggtgttc acactatgga aacggtgata cgccatggtc atggattctg caaaacgctt
     1081 ggcttcatca tagacggcgc gctcaccgac cgggtctaca ttaccaggat atgtttctgc
     1141 ctgcgggtga atttcaggat caccataaat ttcactggta gaggcaagca agaagcgcga
     1201 gccttcacgc cttgcaatgc ccaagcagtt atgggtgcct aaagcgccag ccttcatggt
     1261 ttgaatgggt aaattgaaat aaccagattt tgattgtggg ttggggctgg caggagaggc
     1321 aaaatgcaga atggcatcta ctttgccagg aacgaaaata tagtttgata catcacgttt
     1381 aaaaaattga aacttttcat tccccgccaa gtgggcaata ttatctgggt tgcccgtaat
     1441 aaaattatcc atgccaatga tctcgtgacc atctgctagt aagcggtcgc agagatgaga
     1501 gccaagaaac cctgccgcac ctgtgatcaa aattcgcatt atgttacacc tcttcttttt
     1561 atttacttcc agtccaaccg ggtgatcaat ccatcaccgg tgacttgata ggtattgcta
     1621 ttgccactat cgataagcca aagatttcct tggtacatca cagcaataaa ttcgccactt
     1681 tgcccttcca ccgcagcggg agcccacacg ggtgtttgag gttccagccc atttttatcc
     1741 gcaggcggaa agagtgcccg actgtttgaa ccatcccggt ccatcacaac caaacgatag
     1801 cggcttgagt cgctctggtc gatgaagatg gactgtagat aggcaatctg aaatgccgct
     1861 tctctacttc catattggat cggagaaatg gcaggatatg agaacatgcc tgctgaattg
     1921 accaaagaca ctgccgcctc attcttaaac gagatggctg agaggtcaaa ataaggcgac
     1981 tcctcgccgg aaatgggtgc cggagccgga gcatggctga cgaagtataa accgtttccg
     2041 tctgcacccc aaacgagagg cggaatccac gcccagtcac tatgcgtgtt cagcggagta
     2101 atatctagta atggttttaa gcgaccgcta tcttgatcca ctaagccaat tccatcagga
     2161 cgagaataag cgagcctacc atctggggcg aaggcaaaat tcatgcccca ccaaccatac
     2221 acaccaccac tgcttggata taagatgcgt ttgggtgaac cactgtttgt aatgctgacg
     2281 cgatacaggt cattgtttgc ttgccaacct ggtgccgcac tgcgcggctc aacggtggaa
     2341 tacgcgatcg aattcgtgcc aggaatccat gctgcaaagt ggactacatt ttttgcttgc
     2401 aatgagatca atttagggtc aaggttgcgg gtgcgaactg cccaaagggt attgatctct
     2461 tcgtctgctg gtttgctgga tttgcgcgta aaaaccaaat attcaccgtt aggcgagaga
     2521 gtgaagatgc gcccatctaa atctccggtg ctgacgataa cacggcgatt ggcggtggag
     2581 ccttccatga tccaagcatt gccgccagct agataggcta acgtgccggg aatgtttagc
     2641 ggcacaaaag aagtttgtgc gccgaccatg acaatctcag gtaaagcttc tttgatcacc
     2701 gtagttttta caatggactg actggtttct actttatctt ctaatatctt gcgatatgtg
     2761 atttcttcaa gtccgttcac gccagcctga acgagacggg tttcgccttc gggcaaggtt
     2821 tcgttgcgga cgacttgtcg ttcaaacggg acgacgatct cttcggtttc gaaaacttct
     2881 tccacccgaa ttaaggtgac gaggtcgcca ttgccaatga cggtatagag cggcggctct
     2941 gtcttatcga ggctgtttac ggtgatccct gcactttcca acgcctgtga gacggtttgc
     3001 ccttgcggaa cagtgatgtc tctagtagtg ccatctgcag tgactgtgat tgtaatgtca
     3061 ctattgagct gcggcgactt acaagaagcc aacagtgtaa ccaaaagcaa gatggtggcg
     3121 agcaagctcg ggcggagggt gaggcggggc atgtcgggta taatccagcc gctatttgat
     3181 gcgcccggtc agggtcatga tgccgtctgc gatggtgatg gtttgtaatc gaaagccaac
     3241 agctacggga ccgaatgaac cggtgaaggc ttcttcaata atggcactaa tggcgttgtt
     3301 gacaccttct ggcgcaggga tgggaccaaa gtctgcagat gtgatctgta ttttgggttg
     3361 acctgtggtc tcatcgatcg ttacgttcat ggtaatgagc atattggctg taaaccaacc
     3421 ctgtgtaacc ttgccataca tttgcatttg cccgttgcga agcaagattt gtggatcagt
     3481 aaacggcggg tttgtttgtt gagaaagttt ttctgccata tagccggtta gctgactttc
     3541 ggtgacggaa agggtgatca cgccggtttc tgcaccagtg accatggctt gttggatcat
     3601 atcgcgtagt gcttgggcgt cggttggtga ggctgaaatg ggctgagccg gatattctgg
     3661 tccgccgaca ttgatagagc atgctagtga tgcactgaca agcgaaacaa taaaaaggta
     3721 aatatagttt ttagttttca tgattttgtg aacgtttgaa atatggactg cggagcaatt
     3781 atagcatgag cgaatctgaa cccattacaa atgaagaatt gagcctggcg gcaaaaatcg
     3841 aggcgatgtt attcgtttca gcagacccgg tgccagtgca acaactgtcg caggcgttgg
     3901 atgtgactgc gtctgttgtg gaacgtgggc tgaaagaatt agacgaagca ttaaaatctc
     3961 gcggcttgcg gcttcagcgc aatgcagggc gtgtgcagtt gaccaccgcc cctgagattg
     4021 ctggattggt ggaaaccttc cttgggttgg aggctattac gcacctcagc cgagcagcgt
     4081 tagaaacatt ggcgatcatt gcttaccaac agccggtgac gcgtccacag atcgattcga
     4141 ttcgtggtgt gaatagcgac ggtatgatga aaagcttgtt gagcaaaggc ttgattcagg
     4201 aatctggacg cacagaaggt ccgggtcgtc ctattcttta ttcaaccacg ccggaatttt
     4261 tacagcactt tggtttgagc tccattatgg aattgccgcc attggcaacc ccaattgaaa
     4321 ccgaacctga accgcaagaa ttgttgaaag gttaattatg caggaacgtt tgcaaaagat
     4381 tctcgcacag gctgggtatg gttcccgccg tgcatgcgag gagtttatta ttgaggggcg
     4441 agtttcggtc aatgggcaga ttgcaatctt ggggcagaag gcagatctgg cagtggatac
     4501 gattaaggtg gacggtaagt cgctttcaaa gcctgaaacg atgacgtata tcgccttata
     4561 caaacctcgt aatgtcctct ctgctgcaga ggggcaagac gatcgtaaaa cggttcgaga
     4621 tttgattccc gctcctggac atttatatcc agtaggacgc ctcgattttg actccgaagg
     4681 cttgattctc atgaccaatg atggggatct cacgaacaag ttgacacatc cgcgatatgc
     4741 acatgaaaaa gaatatcgcg tgctgctcgc tcgtaaaccg gatactgaac agcttgatgc
     4801 ttggcgccga ggcgttgtgt tgccagaagg tgaccgtacc cagcctgcga atgttcactt
     4861 tatttccaca tctggcaaag gtgcatggat tcgtgtggtg atgggggaag gcaagaaacg
     4921 tcagattcgg gaagtgggta aattgcttgg tttgcctgtg gtacgcatta ttcgtgtgcg
     4981 tatcggcaca ttaaaattag gtgatttgaa accccgacaa tggcgatatt taactgagga
     5041 tgagattcgg gaattaaaag gggataaggt tccgaagatg gagcaaaagc cacgcccgaa
     5101 acgaacaaca acccgtcggg cgtaacggtc tcttacaagg tactgtaatg aaaagactca
     5161 attttatagt cattattgca ctggtagctt ccacgttagg agcttgccag gtccttccgc
     5221 gtatcaatga tacacgcccg aatttcctca tcattgtgac agatgaccag cgttatgaca
     5281 caatggaata catgcctaac actcaagaat taatatttga ccagggcgtg accttttcca
     5341 aaggatacat caccactccg ttttgttgtc cgagccgtgc cagcattcta accgggttgt
     5401 atgctcataa tcattgggtg caggtcaatg aagacccact cgaattccgg acgatggtgg
     5461 aagatattca ccgcaacggc tattacactg gcttggttgg gaaatacctg aattcatgga
     5521 aaggggatat gcgccccgaa tatgattttt gggtctcatt ttggggtgga accatgaaga
     5581 actattacga tcccgatttg aatgtgaatg ggacatggtc caaacatacc gggtattcaa
     5641 cctatttgtt tagagacttt ggcgttcagt tcattgaaga agctagtaag cagtcgaagc
     5701 cttttcttct gatgtatacg ccaaatgcgc cacatgctcc ttttacgcct gcggaagaag
     5761 atgtcagttt actaactgac ttggctcctt tccgcccatt gagtttcaac gaagaagatg
     5821 tttcggataa gccagattcg atctccaata tggctccctt atccgaagag gatattaagt
     5881 ctattgataa tacgcgtaga cgtcagttgc tgactctcat ggcgcttgac cgcaccatcc
     5941 ctgacctcat caataaattg aaagaaacgg gcgagttaga taacactgtc attattttta
     6001 tctcggataa tggcaagcat tggggcgagc atcgcttgga ctcaaaaagc acagcgtatg
     6061 aagaatcggt taaggtgcca tttgcgatac gctatccacc actgatccca actccatatg
     6121 tggataatcg catcgttgct aatattgata ttgcgccgac gatttatgaa ctatctgaaa
     6181 cgcgtttgcc ggacttaatg gacgggactt ctttagtttc ccttttttcg ggggaaaccg
     6241 aatggcgaga ccacatcctg ctcgaagcct ggccagaccg tggtgcttgg tctgccattc
     6301 atacgggtga ttacgtttat attgaaacac agggcgatct gtctgagttt tatgatttaa
     6361 cgcttgatcc cttcgaaatg gacaatgcca tcaatgaccc acagtatgca gagatgattg
     6421 cccaattaca gcaatacctt caaacagaga agcagccaaa aacttcgtcg ccttaatagt
     6481 tacatttctc ccttgactct gtcctattca aaccttagaa tttgaataac aattgaacgg
     6541 attgcaggga agagatcccc gcaagaggac gccgaaggtg caaatctgga atcaggcgaa
     6601 accagatgaa tctctcaggc aaaaggaccc tgcaaccgca accactctgg aaaactcccc
     6661 gccaaaggga taccgacggt gtaaagttag ttcggtagtc agatagtctg atagtgaacg
     6721 cgttaggtag tcatatacat ggcttcaaaa caaaagagct attagatgaa ataactacaa
     6781 gactacccca atctctcagg ttccattaca gaggacgcgc gataataata ttgcgcgtcc
     6841 tcttttgatt cgccaatacc tcacactagg agacataaaa tgaacaaccc caccaacctc
     6901 aagtacgcga aatctgacga atggtttgac ccatccactg gcgccgtcgg catttctgat
     6961 tatgctcagg gacaactctc tgatattgtt tttgttgaaa ttctggttga cgaaggggag
     7021 gaagtgactg ttggaaaagc tattgcttcg gtggagtctg taaaagcttc gtctgaaatt
     7081 tatgctccgg tcgcagggaa ggtcactgct gtgaataaag acctctccaa tactcctgaa
     7141 accattaaca ccgatcccta tggcgcggcg tggatgatca agatcgatgg tagcgcttct
     7201 gccgatttaa tggatgctgc tgcctacgaa acccattgtg ccggaagaag tcactaacta
     7261 ttgcattcag ccatcagcct attcttgaaa gctgacgact gatcgctttg gagttttatt
     7321 atgacctata ttccaattag cccgaaagaa cgggatgcga tg
//
LOCUS       DAOQJE010000048         7351 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_5572_length_7351_cov_6.547834, whole genome shotgun sequence.
ACCESSION   DAOQJE010000048 DAOQJE010000000
VERSION     DAOQJE010000048.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 7351)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7351)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7351
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5572_length_7351_cov_6.547834"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..431)
                     /locus_tag="PLT08_02195"
     CDS             complement(<1..431)
                     /locus_tag="PLT08_02195"
                     /inference="COORDINATES: protein motif:HMM:NF016750.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocation signal
                     domain-containing protein"
                     /protein_id="HNE03301.1"
                     /translation="MSENFSRRDFLKLAGLGAATTAILTGCGPASRYVKREPYQQMPE
                     YNSVGQSTYYATTCRECAAGCGLVVRTYQGRALKTEGNANHPLNLGKTCARGQATLHG
                     LYNPDRVQGPSTGDWDSAIQTVADAFKTYKPSEIAFLMGMAP"
     gene            complement(453..1010)
                     /locus_tag="PLT08_02200"
     CDS             complement(453..1010)
                     /locus_tag="PLT08_02200"
                     /inference="COORDINATES: protein motif:HMM:NF025875.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c3 family protein"
                     /protein_id="HNE03302.1"
                     /translation="MIKKFFDWLKQPLGLIAVGIVFLAVFSAAAYGLWTTQQAPAQPV
                     PGFPHKTHVAFGIQCLYCHPGAAKGPAAGIPSQAKCWACHNQIVKTRDSALLAPLKDA
                     VLSGTSLEWVPVAIVPDFVQFNHRAHIAAGKNCEECHGDMTQVTIAQNPQVMNMGWCL
                     NCHIQNAGEDQAKLIKLTDCGTCHY"
     gene            1396..1644
                     /locus_tag="PLT08_02205"
     CDS             1396..1644
                     /locus_tag="PLT08_02205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE03303.1"
                     /translation="MKTKNIFNACIFLIAILTLTLVITNFNMPVAAAKDLPVTVLQQA
                     TPTPPVDSSEIGSTSGILVMGVVIVLIITVPLILQKRK"
     gene            1721..2428
                     /locus_tag="PLT08_02210"
     CDS             1721..2428
                     /locus_tag="PLT08_02210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HNE03304.1"
                     /translation="MPEKILIVEDEIALQETLAYNLKKEGYTVETVGDGRSALEAARK
                     LKPDLIVLDIMLPEMDGFEVARTLRKEMTTGILMLTARDDEIDRVVGLEVGADDYLTK
                     PFSMRELIARVKAQLRRARLLREEMGKSVEETPHETLTFDNLIINLTRREVTLNGTPV
                     QLKPKEYELLLFLAEHRRQMLSREFILERVWGWDYIGDSRTVDVHIRWLRQKIEESSS
                     EPKRIVTVRGGGYRFEG"
     gene            2478..3716
                     /locus_tag="PLT08_02215"
     CDS             2478..3716
                     /locus_tag="PLT08_02215"
                     /inference="COORDINATES: protein motif:HMM:NF020037.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNE03305.1"
                     /translation="MNQFAYATAIVFLIIAAWFAWKYYSLRKNVNQLAKSLHDPKIPL
                     PHLREIEELLSMIESRKATFSAELFSLQTENERLATVLEQLTDGVLIADADGRVQFAN
                     PAASKLFGTHNPTQQSVAQVVRSHQLIEAWKRCQQTRQLQIEAVEIATRKQYLQIIVI
                     PDRHEGGSLLLAQDLTQVRKLETVRRDFISNVSHELRTPLASLKALTETLQGGALADP
                     EAGPRFLDRIHTEVDALTQMTQELLDLSRIESGQVQLNFEMISPRKLIYSAADRMKAQ
                     VERAHLKLDVKCEDGLQNIKADKNRLEQVLVNLIHNAVKFNKPGGSITLSAEPTSGGV
                     RCAVRDTGVGVPMESLNRIFERFYRVDSSRTGSGTGLGLSISKHIIEAHGGKIWAESE
                     EGRGSTFFFIVPLDPSKAKE"
     gene            complement(3679..>4436)
                     /locus_tag="PLT08_02220"
     CDS             complement(3679..>4436)
                     /locus_tag="PLT08_02220"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HNE03306.1"
                     /translation="ARTAELTVVNADLEKAMRAKDEFMAAMSHELRTPLTGILGGAGA
                     LQGSTDDTLSDKQKKSVNLIERNGHRLLSLVNDLIDYSRLQSGKQILFKSPYALTDIC
                     IAVLRSVETDAKRKKQSVSLQSQPEGIIVDTDVTLMKKMLIHLLRNASKFTPEGGEFG
                     ISLTEKSEQHCVEVVVWDTGIGIKKSDIANIFTPFVQLDATLARKYEGTGLGLAIVNK
                     LVELFDGKMDVFSTPGSGSRFVILLPWKGPTEQ"
     assembly_gap    4437..4536
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<4537..5106)
                     /locus_tag="PLT08_02225"
     CDS             complement(<4537..5106)
                     /locus_tag="PLT08_02225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE03307.1"
                     /translation="MTQKDISFFPKALNLRFEFLKRPVQKTYAPYVFLLLLIIFPPLL
                     IRSKTIYPFYGDLFACIWSAGETYVLFYVAARYRPTSKQLGRAWTIIATSALFSFLGN
                     LFWFGMSHFLHIYPYPSVADIFYLLSYPLMLIGILSFPYTSKTTLEKLKIYLETATVV
                     ISIGMALWFFVLRPVIDQTGTSDKTYLFFT"
     gene            complement(5229..5573)
                     /locus_tag="PLT08_02230"
     CDS             complement(5229..5573)
                     /locus_tag="PLT08_02230"
                     /inference="COORDINATES: protein motif:HMM:NF013868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HNE03308.1"
                     /translation="MELNVSDLDKGITLIKLDGRLDITGVNQIETKFAAYCSGENARV
                     IVDMAKVDYLASIGIRLLVTNAKSLKSRNGKIIILRPTQSVMEVLEMTNIPAIIPVYT
                     NLESAEAVLLGY"
     gene            complement(5651..>7351)
                     /gene="ppk1"
                     /locus_tag="PLT08_02235"
     CDS             complement(5651..>7351)
                     /gene="ppk1"
                     /locus_tag="PLT08_02235"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR03705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009358 - polyphosphate kinase complex
                     [Evidence IEA];
                     GO_function: GO:0008976 - polyphosphate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006799 - polyphosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 1"
                     /protein_id="HNE03309.1"
                     /translation="RADQYFKDVVYPVLTPLALDPGHPFPHISNLSLNLAIVIRDKKG
                     NEKFARLKVPDTLPRLIPIKKSSGSARKDGTIPFHHYFVWLDQVIAANLDGLFPGLEV
                     VSAHPFRIVRDADIEIQELEADDLLETMQQNIRKRKFGSVVQVAVHPSMPEEMRDLLV
                     DNLEITRNDIYSLPHPLGLASLWQLYNSVERHDLKYPPYIQRTPKPLRNLDTSSEIFD
                     AIRNENVLLHHPYESFIPVVDFLYAAARDPHVLAIKQTVYRVGSNAPVVEALLEAAER
                     GKQVAVLVELKARFDEESNIGWARALEQAGVHVVYGLVGLKTHCKVAMVVRKEGDGIR
                     RYLHLATGNYNAVTSRIYEDLGMFTCDEDMGADATDLFNYLTGYSTKKSYKKLLVAPV
                     NLREQMEKLIRREIEHAKAGNKARIIFKMNSLVDMDMIQLLYQASQAGVKVDLLIRGM
                     CCLRPGIKGVSENIRVLSVVGRYLEHSRIYYFQNNGNDEIYMGSADLMPRNLNHRVEV
                     VFPVENKGQVKFLRDKILEAYLKDNTRARILKTNGTYERSKPDSNGKRFDVQSHLMGE
                     "
BASE COUNT         2004 a         1717 c         1670 g         1860 t
ORIGIN      
        1 ggtgccatac ccatgaggaa agcgatctca ctgggtttgt aggttttgaa ggcatcggct
       61 acggtttgaa tagcagagtc ccaatctccg gtggaaggac cttgaacacg gtctgggttg
      121 tataagccgt gaagagtggc ttgcccgcgt gcacaggtct taccgagatt gaggggatga
      181 ttggcattgc cttcggtctt gagagcacga ccctgatagg tgcgcacaac gagaccacat
      241 cccgccgcgc attcgcggca tgtagtggcg taataggtac tctgcccgac cgaattgtat
      301 tcaggcatct gctgataagg ttcgcgctta acataacgcg aagcgggacc acaacctgtg
      361 aggatcgcag tggtcgccgc tcctaaacca gccagtttta gaaaatcacg ccgggaaaaa
      421 ttttcgctca tataggtttc ctttcacccg gtttaatagt gacacgttcc gcagtctgtc
      481 agtttgatca atttggcttg atcttcaccg gcattctgga tgtgacaatt caaacaccag
      541 cccatgttca tcacttgtgg attttgggca atggttactt gagtcatatc gccgtgacat
      601 tcttcacagt tcttccctgc cgcaatatgc gcacgatggt tgaactgtac aaagtctggc
      661 acgatggcaa cggggaccca ttcaagcgat gtgccactca ggactgcgtc cttaagtggg
      721 gcaagcaaag cactatcacg ggtcttaacg atctgattgt ggcatgccca gcactttgct
      781 tgtgatggaa tgcccgccgc aggacccttc gccgcgcccg gatggcaata caaacattga
      841 ataccgaagg caacatgggt cttatgaggg aaccctggaa caggttgagc aggtgcttgt
      901 tgggtagtcc ataacccata agcagccgcg ctaaacactg ccaggaacac gatccccacc
      961 gcgatcagac caagaggctg tttcagccaa tcgaaaaact ttttgatcat ggatgctcct
     1021 gaaataaact aagcgtaacg ccgccattgt tgccaatggt ggcgtttgca aaattaccaa
     1081 cttcttaatg acaaacatgt agcgcttttg ctggtctctc tctttataaa aagaacccac
     1141 ccaaattact ttacaagaca gccgcgctct gagggtattt ttcgtgcatc tgcctgcact
     1201 gatgcacaac ttgtaaatta tacatcgaac gtgcaaaaag gtacaagtta tccgatttgt
     1261 gttttaagcc caaacaggac ttttttggtc aaaatttctc aacttaacac actttaatat
     1321 tgatgtgatt ttgatgttat gttttaaaag aacactccga acaaaagaat ggtttcacct
     1381 tataatgaac ctacgatgaa aacaaaaaat attttcaacg cgtgcatttt tttgatagcc
     1441 atcctaactc tcacgttggt gatcacaaac tttaacatgc ctgtagctgc cgccaaagat
     1501 ttgccggtca ccgttttaca gcaagccacc cctacccctc ccgttgacag ttcagagatc
     1561 ggctccacaa gcggaatcct ggttatggga gtggtcatcg ttctgatcat cactgtgccg
     1621 ttgatcctcc aaaaaagaaa ataaaacgga ttggcttgaa gttcaagcca ttaacctcac
     1681 cttaacagat cagggattcc tttatggtat agtgccactc atgcctgaaa agatattgat
     1741 cgttgaagac gagatcgctc ttcaagaaac ccttgcctat aacctaaaaa aagagggcta
     1801 taccgttgaa accgtaggcg atggtcgctc tgcgctcgaa gctgcccgta aactaaaacc
     1861 agacttgatc gtgctcgaca ttatgctgcc agaaatggat ggcttcgaag tggcgcgcac
     1921 attacgcaaa gaaatgacaa ctggcattct gatgctcacc gcccgcgatg acgagatcga
     1981 ccgtgttgtt gggctggaag tgggcgccga cgattaccta accaagcctt tctcgatgcg
     2041 tgaattgata gcacgcgtca aagcgcaatt acgccgtgcc cgcctgttaa gagaagagat
     2101 ggggaaatct gtagaagaaa cgccacacga aaccctcacc ttcgataacc tcatcatcaa
     2161 cctcacccgc cgcgaagtga cattaaacgg cacccccgta caactgaagc caaaagaata
     2221 tgagcttttg ctctttcttg ccgaacaccg ccggcaaatg ctgtcacgtg aatttattct
     2281 ggaacgcgtt tggggttggg actatattgg cgatagccgc actgtcgatg tgcatatacg
     2341 ttggttgaga caaaagatcg aagaaagttc aagtgagccg aaacgtattg ttaccgtccg
     2401 tggcggcggg tatcggtttg aaggataaag tgtgatgagt agcctacgcc tctctcagta
     2461 cgtattgtga cccacgtatg aatcaatttg cttatgcaac agctatcgta tttctcatca
     2521 ttgcggcgtg gttcgcatgg aagtattaca gcctgagaaa aaacgtcaat cagttggcga
     2581 aatctctgca tgaccctaaa attccgctcc cccatttaag agaaattgaa gaactgctta
     2641 gcatgattga gtcgcgcaag gcaactttct ctgctgagct tttctctctg caaacagaaa
     2701 atgagcgcct tgctacagtc ctcgaacagc ttaccgatgg cgtgctcatt gccgatgccg
     2761 atggccgggt gcaatttgca aaccctgcgg caagcaaact ctttggcact cacaacccca
     2821 cacaacaaag cgtggcacag gtggtgcgca gtcatcaatt aattgaagcg tggaagcgct
     2881 gccagcaaac ccgtcaactt caaattgagg cagtagaaat cgccacacgc aaacagtatt
     2941 tgcagatcat cgtcattcca gaccgccacg aaggtgggag tttattgctc gcgcaagacc
     3001 tcactcaggt gcgcaaactc gaaaccgtac ggcgggactt tatctcgaac gtctcccatg
     3061 aattgcggac gccgcttgct tcgctaaagg cgctcaccga gaccttacaa ggcggcgctc
     3121 tcgcggaccc ggaagctggt ccgcgtttct tagaccgaat ccacactgaa gttgacgcgc
     3181 tgactcagat gacccaagaa ctgctcgacc tttctcgcat tgagtctgga caagtgcagc
     3241 tcaactttga aatgatctcg ccgcgtaaac tcatctactc tgccgcagac cgaatgaaag
     3301 cacaagtgga acgtgcgcat ttgaaattag atgtaaagtg tgaagatggg ttgcaaaata
     3361 tcaaagccga taaaaatcgg ttggaacagg tgttggtcaa cctgattcac aatgcggtca
     3421 aattcaataa gcctgggggg agtatcactc tgagtgcaga gccaacttcg ggcggggtac
     3481 gatgcgcagt gcgggataca ggtgtaggag tccctatgga gagtctcaat cgcatctttg
     3541 aacgcttcta tcgcgttgat tcatcccgca ccggttctgg cacaggatta gggctttcga
     3601 tttcaaagca tattatcgaa gcgcacggcg ggaagatttg ggcagaaagt gaagagggac
     3661 gcggcagtac atttttcttt attgttccgt tggacccttc caaggcaaaa gaataacaaa
     3721 acgacttcca gaaccggggg tgctaaaaac gtccatcttg ccatcaaaca actccacgag
     3781 tttatttaca attgcaagcc caagccccgt gccttcatat ttacgcgcta atgtagcatc
     3841 taattgaaca aagggagtga agatgttggc aatatcactt ttcttaatgc caattcccgt
     3901 atcccaaacg acgacctcaa cacagtgttg ttcagatttt tcggtcagcg aaatcccaaa
     3961 ctctccgcct tcgggggtga atttactggc atttcgcagc aaatgaataa gcattttttt
     4021 catcaatgtc acatcggtat ctacaataat gccttcaggt tgagattgta acgaaacgct
     4081 ttgcttcttg cgcttggcat cggtttctac agaccgtaat actgcaatac agatatccgt
     4141 aagagcatat gggctcttaa acaaaatttg ttttccgctc tgcaaacgtg aataatcgat
     4201 caggtcgttc acgagagata aaaggcggtg tccatttctc tctataaggt tcactgattt
     4261 cttttgttta tcgctcaacg tatcgtcagt tgaaccttga agggctccgg ctccacccag
     4321 aatgccagta agcggggtgc gcaattcatg actcatggca gccatgaatt catcttttgc
     4381 ccgcatggct ttctccaagt ccgcattgac cacggtcaac tcggctgtac gtgcctnnnn
     4441 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4501 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnntgta aagaacaagt aggtcttatc
     4561 tgatgtccct gtttggtcaa taactggtct tagcacaaag aaccaaagtg ccattccgat
     4621 tgaaataacc acagttgccg tctcgaggta aattttgagc ttttcaagag ttgtcttact
     4681 ggtgtatgga aaacttaaga ttccaatcaa catcagcgga taagataaga ggtaaaagat
     4741 atctgccaca gatggatatg gataaatatg taaaaaatgt gacataccaa accaaaacag
     4801 attccccaaa aagctaaaga gggcagaggt tgcaataatc gtccatgccc gccctaattg
     4861 tttggaggtt ggtcggtagc gagccgccac ataaaaaaga acataggtct caccagcact
     4921 ccaaatgcaa gcgaacaaat caccataaaa aggataaatg gttttggagc ggattaagag
     4981 aggggggaaa ataattaaca acagcaagaa aacatagggc gcatatgttt tttggacggg
     5041 tcttttaagg aattcaaacc tgaggtttaa agctttagga aaaaaggaaa tatctttctg
     5101 tgtcatgtga atctcatttc atatctgagc aactacttaa attcaaacat gactccccaa
     5161 acattcctag tagggaaaac atctatttcg gagtaaatcg gtctgtatat ttcccccgtt
     5221 acattgagct agtacccgag caaaaccgcc tcagcagatt caagattggt atagacaggg
     5281 atgatggcag gaatatttgt catttcaagc acttccataa cggactgggt agggcgcaaa
     5341 ataataattt tgccatttcg actcttgagg gatttggcat tggtcaccaa caaacgaatg
     5401 ccaatagaag ccaaatagtc cacctttgcc atatcgacga tcacacgtgc attttcaccg
     5461 gagcaatatg ccgcaaattt tgtttcgatc tgatttacac cagtaatatc tagcctgccg
     5521 tctaatttga ttagtgtaat acccttatct aagtcgctga cgtttaattc catagaagtc
     5581 acctttgttt tgaagatggt tcattatatg aacgttaaca ttattgattg ttaacaaaag
     5641 gttatggttt ctactcaccc atcaaatgag attggacatc gaaacgcttc ccattagaat
     5701 ctggtttgga acgctcatag gtaccgttcg tttttagaat gcgcgcccgg gtattatctt
     5761 tgagataggc ttccagaatc ttatcgcgca agaatttgac ttgtccctta ttttcaactg
     5821 ggaaaactac ttcgacacgg tggttaaggt tgcgaggcat aagatcagca ctacccatat
     5881 aaatttcatc attgccattg ttttggaagt aataaatacg gctatgttca aggtaacgcc
     5941 cgacaacact tagcacgcgg atattttcac tgacaccctt aataccgggg cgcaaacagc
     6001 acatgccgcg aatgagcaaa tccactttga cgccagcctg agaagcctga tagagaagct
     6061 ggatcatatc catatcaacc agtgaattca tcttaaagat aatgcgtgcc ttgtttccgg
     6121 ctttcgcgtg ttcgatctca cgtcgaatca gtttctccat ctgctcacgc aaattgaccg
     6181 gggcaaccaa caatttctta taacttttct tggtagagta accagtgagg tagttaaaca
     6241 agtctgtggc atcggcgccc atatcttcat cgcaagtgaa catgcccaaa tcttcataaa
     6301 tgcgggatgt gaccgcattg taattgccgg tggcaagatg caaatatctg cggatgccat
     6361 ccccttcttt gcggacaacc atggctactt tacagtgggt cttcaaccct actaagccat
     6421 agacaacatg aactcctgcc tgctccaagg ctcgcgccca accaatattt gattcttcat
     6481 caaagcgggc tttcaactcc acgagtacgg caacttgttt accgcgttct gctgcttcta
     6541 gcagagcttc aaccacaggc gcattggacc ctacccgata caccgtctgt ttgatggcaa
     6601 gcacatgcgg atcgcgcgcg gcggcataaa gaaaatccac gactgggatg aacgattcgt
     6661 aagggtgatg aagcaaaaca ttttcattcc gaatcgcatc aaatatctct gaagatgtat
     6721 ccagatttcg gagtggtttt ggtgtgcgtt gaatataggg cggatatttc aagtcatgcc
     6781 gttcgacgct gttatacaac tgccaaagac tcgccaaccc aagcggatgc ggcaatgaat
     6841 aaatatcatt tcgggtaatt tcaaggttgt ccaccagcaa gtcgcgcatt tcctctggca
     6901 tggatgggtg aactgctacc tgtaccaccg agccgaattt gcgtttacga atgttctgtt
     6961 gcatggtctc taacaggtca tcggcttcga gttcttggat ttcaatatct gcatcgcgga
     7021 caatgcggaa gggatgtgca gagaccactt ccaagccagg aaaaagccca tcgagattgg
     7081 cagcaatgac ttggtctaac caaacgaaat aatggtggaa ggggatcgtc ccatctttgc
     7141 gggcgctgcc agaagatttt ttgatgggga tcaagcgcgg taaagtatca ggcactttga
     7201 ggcgtgcaaa tttttcattg ccctttttat cgcggatgac gatggcgaga ttaaggctca
     7261 ggttggaaat atgcgggaat gggtgtccag gatcaagcgc gagcggagtc agaaccggat
     7321 agacaacatc cttgaagtac tgatcggcgc g
//
LOCUS       DAOQJE010000049         7268 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_5655_length_7268_cov_6.464023, whole genome shotgun sequence.
ACCESSION   DAOQJE010000049 DAOQJE010000000
VERSION     DAOQJE010000049.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 7268)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7268)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7268
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5655_length_7268_cov_6.464023"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1670)
                     /locus_tag="PLT08_02240"
     CDS             complement(<1..1670)
                     /locus_tag="PLT08_02240"
                     /inference="COORDINATES: protein motif:HMM:NF015714.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0030420 - establishment of competence for
                     transformation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ComEC/Rec2 family competence protein"
                     /protein_id="HNE03310.1"
                     /translation="MPLLWISLSFLTGIVIAAALPLPTWAWVLIALTFFLLFIFLRRS
                     QFATRFSFLNTFPFIPLLPLFLFLGSAYYQARQPKLDAFHVAFYNDRTYDLLITGYIE
                     DAPDYRDSYTNLILNVEAVDSGDGDLPVSGRLLVRVEPNETYEYGERIRLRGKLKTPP
                     ENEEFSYRDYLARQGIHSYMTIAEVTRLPGNDGNPIYKLIYGFKEQLVSHTYKIFPDP
                     EASLLSGILFGVDTGLPKKLQDDFKTTGTAHIIAISGFNIAIIAGLFFRFFKWTLKNE
                     RLGAVFAVIFVFLYAFLVGGDPAVIRAAIMGSFSLFAKQVGRRNAGMNTLAVVAALMA
                     LWNPLTLWDVGFQLSFFATLGLILYAEPLSNITVRFFEKVTQQEAGAIANIINDNVML
                     TIAAQIMTIPLMLYYFHRLSLISFIANPFILPVQPAVMILGGLAVFVSLLLQPIGQLL
                     AWVAWPFAGYTIRVVEFFAKTPNGVIVFGDVPLWFIYMTYTSLLLVTFNWSAIQNWFK
                     SAASSLRAIALTLAFSIAFICMVTFWSASAKSGDGQFHIIFLEVGSADA"
     gene            complement(1697..2239)
                     /locus_tag="PLT08_02245"
     CDS             complement(1697..2239)
                     /locus_tag="PLT08_02245"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014526.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNE03311.1"
                     /translation="MNWKLIPEPELSPTLEADIQQMLIASFPDYTDFFTANSYWGTKP
                     EFRLIALENNQPIAHLEFGYRQISVGNMSVNIAGIGAVAIHPRHQGKKLGKLMFAHLR
                     EYLLRNSNVDFGFIGCHEEVAGFYAASGFQRVDQSVYNLNPDSQEWETFHGPTLVMPI
                     HKSMQEWRHGELIHLHGMPW"
     gene            2299..3771
                     /locus_tag="PLT08_02250"
     CDS             2299..3771
                     /locus_tag="PLT08_02250"
                     /inference="COORDINATES: protein motif:HMM:NF017206.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HNE03312.1"
                     /translation="MNQPKLILVTGATGYVGGRLVPKLLEAGYRVRCLVRDVSRLQGR
                     AWSKKVEVVAGDALNLDELASAMKDVSVAYYLMHGKQGGKDNAERDLKAARNFAQAAE
                     DEGIEQIVYLGELVDPTANLSPYLRSRHETGFVLRHGKVPVTEIRAGMIIGSGSALFE
                     MIRYLTEREPVLTCPAWFFSQAQPIAIRDVLSYLVDTLKTPDVIGRVIEVGGPTRLTY
                     ADMLLGYAKERNLRRWLIRTPFYAPRLSAYWVHMLTPIHWRVVAPLIEGLSAELIVRD
                     ETAKKLFPHIQPIDFQTAVHLALGRVQRDNVETSWADALVTEAGDVQPYVFTVEDGLF
                     IERRQVVLELSPETVFRSYTGIGGARGWMYMDWAWAIRGWMDKAIGGVGLRRGRRHPD
                     EINTGEALDFWRVELVEKFRIMRLRAEMIVPGKAWLQFESTPLEEEKGKTIFTVTAYF
                     EPYGVPGFLYWYSMWPFHKFIFNGLARRLASRARVLGHAY"
     gene            3837..4445
                     /locus_tag="PLT08_02255"
     CDS             3837..4445
                     /locus_tag="PLT08_02255"
                     /EC_number="2.3.1.275"
                     /inference="COORDINATES: protein motif:HMM:NF014690.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043772 - acyl-phosphate
                     glycerol-3-phosphate acyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate acyltransferase"
                     /protein_id="HNE03313.1"
                     /translation="MNILFPLLGYIFGSLTFSIWITRYVKQVDVRDSGSGHATTTNTI
                     RQAGFGWGALVLILDIAKGFLPTYLATKYSGETWVIALTATAAVIGHCWPVFAQFRGG
                     MGLATAGGSVLAVNPLAFVICLGLLILLVLVVRHSARASVFAGILFAPVLWLLNIRDV
                     AFWVAVGAGAVIAVRFLIDWNRKYRELWLDREKKSEKVEKAE"
     gene            complement(4506..5369)
                     /locus_tag="PLT08_02260"
     CDS             complement(4506..5369)
                     /locus_tag="PLT08_02260"
                     /inference="COORDINATES: protein motif:HMM:NF025053.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein phosphatase 2C domain-containing
                     protein"
                     /protein_id="HNE03314.1"
                     /translation="MIRTTLAHLHIAALSHAGISGKNNEDRYAVSSFQLSKDDPRPST
                     FAVVADGIGGHRAGEVAAELAVNYISMHIAESNARKPIKILENAIHDASQAIATHSAG
                     KAEEQGMGSTCACVWIVENKLYTAYVGDSRIYLLRGKYIQQITIDHTWVQEAYEKGII
                     TADQMRDHPNVHVIRRHLGGIRLPEVDFRLRLDNNETEEESLNNQGFHLQPGDTILIC
                     TDGLTDLVWDDEIKDIIRGKHDLKTAAETLVNLANERGGHDNITVVLLAMPRVEETLP
                     KKSILGWLTGE"
     gene            complement(5369..>7268)
                     /locus_tag="PLT08_02265"
     CDS             complement(5369..>7268)
                     /locus_tag="PLT08_02265"
                     /inference="COORDINATES: protein motif:HMM:NF028007.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HNE03315.1"
                     /translation="SISLLFGVVIVPAQAQGSVTASVTIYNVDASNFPSVTGFVDVTD
                     ANGIFASGLKPEAVTLIENGQPIPADSFNEIAIPLQLVVAVNQGEALGAQNANGISRY
                     QRVEQIIEQWAQTRPADLPDDLSLVSQAGPVINHAPPADFVVGLKGFQPDLRTATPNI
                     QSLVSALDVVSAQTPRIGVKRAILFITPQMTDPNLAASVEPLIQRALENKIRVFIWYV
                     DANTTFTTTSAAVFNNLAIQTGGTMFQFSGEERFPDPEEYFSPLRRIYNFSYTSRVNT
                     SGNHTVAVQVNLPSVGPLNSETLPFTLNIQPPNAFPVAPSIQITRQAPEEDPFNTEVL
                     LPETQEIEIIIEFPDGFDRPLARTTLIVDGIAVDENTAEPFTKFTWDLKPYTISGEHQ
                     LTVEAVDSLGLSKTSMPLPVVVTVIKPPSGPAAFLAKYRMPLTFGAIILAGLVLFAIL
                     LSGRLRIRSLRVMQEERRVQNDPLTQSLEAFTEPAPVAVKEKTKKPRTKSTKAKAAKT
                     LENEALASLVRLQADGQVAAVPPIQLNDAEIVFGADPVQCSQILDDPSISSVHARIRA
                     TEDGGYLLMDAQSLAGTWVNYEPIPREGYRLLHGDMVNFGQLKYRFMLRTPPPPRTPR
                     ITPVTPED"
BASE COUNT         1750 a         1621 c         2092 g         1805 t
ORIGIN      
        1 gcatctgcgg aaccaacttc gagaaagatg atgtgaaatt gaccatcgcc agattttgcc
       61 gaggcactcc aaaatgtgac catacagatg aaagcgatgg aaaaggctaa agtcaacgcg
      121 atggctcgaa gagatgaagc cgcagacttg aaccagtttt ggatggcaga ccagttaaag
      181 gtcacgagaa gaagcgaagt gtatgtcata taaataaacc aaagagggac gtctccgaat
      241 acgatcacac cgtttggcgt tttggcgaaa aattccacca cacgaatggt gtagcctgca
      301 aagggccatg cgacccatgc aaggagttga ccgatgggtt gaagaagcaa actaacgaaa
      361 actgccagcc cgcccagaat catcaccgct ggttgaacgg gcagaatgaa cgggtttgcg
      421 atgaatgaga tcaaagacag gcgatgaaaa taatataaca tcaaagggat agtcatgatc
      481 tgcgcggcga tggtgagcat gacattatcg ttgatgatat tcgcaatggc gccggcttct
      541 tgttgggtga ctttttcaaa gaagcggact gtgatgtttg agagtggttc agcgtatagg
      601 atcagcccaa gtgttgcgaa gaaggagagt tgaaacccta cgtcccataa ggtgagcgga
      661 ttccataacg ccatgagtgc agccacaaca gccaacgtat tcatgcccgc attgcgcctg
      721 ccaacttgtt tggcaaagag cgagaaactg cccatgatgg cggcgcgtat cacggctggg
      781 tcaccgccta cgagaaaggc atataaaaaa acaaagatga cggcgaatac tgcgccgagc
      841 cgttcgtttt tgagcgtcca cttgaagaag cggaagaata gaccagcaat gatggcgatg
      901 ttgaacccag agatggcgat gatatgcgcc gtgcctgtgg ttttgaagtc atcttgtagt
      961 ttcttaggca gacccgtatc tacaccgaag agaatgccag agagcagcga ggcttctggg
     1021 tctggaaaaa ttttataggt gtggcttacc agttgttctt tgaagccata gatcaatttg
     1081 taaatggggt tgccatcgtt gccagggaga cgagtcactt cggctatcgt catatacgaa
     1141 tggattccct gacgggcgag ataatcgcga tacgaaaatt cttcgttctc gggcggagtt
     1201 ttcagctttc cgcgcagacg gatacgctcg ccatattcgt aggtttcgtt tggctccaca
     1261 cgcaccagca gccgacccga aactggtagg tcaccatcgc cgctatccac ggcttcgacg
     1321 ttgaggatga ggttggtata ggaatcacga taatcgggcg catcttcgat gtatcctgtg
     1381 atgagcaggt cataagtgcg gtcgttgtag aaggcgacat gaaacgcgtc taacttgggt
     1441 tggcgggctt gataataagc agaaccaaga aaaagaaaga gagggaggag cgggatgaat
     1501 gggaaggtat ttaggaatga gaaacgagtt gcaaattgag aacgccgaag aaagataaaa
     1561 agaagaaaga aggtaagcgc gatcaagacc catgcccaag tggggagagg gagtgctgct
     1621 gcaatgacaa ttcccgttag gaaagataaa gagatccaca ataagggcat gaaaggattc
     1681 tactttagtt tagtcatcac cacggcatac cgtgtaggtg aatcaattcg ccatgacgcc
     1741 attcttgcat ggatttgtgg atgggcatca ccagagtcgg accatgaaag gtttcccatt
     1801 cttgagaatc tgggttgagg ttataaacac tttgatccac acgctgaaaa ccagaagcgg
     1861 cataaaaccc cgctacctct tcatgacagc cgataaagcc aaagtcgaca tttgagtttc
     1921 tcaaaagata ttcacgcaaa tgagcaaaca tcaacttgcc aagttttttc ccttgatgtc
     1981 tggggtgaat tgccacagca ccaattccag caatgtttac agacatattt ccaacggaga
     2041 tttggcgata cccgaattct aaatgggcaa tgggttgatt gttttccaag gcgatcaacc
     2101 gaaattcagg ctttgtgccc cagtaggagt tggcagtgaa gaagtccgta taatcgggga
     2161 aggaagcgat taacatctgt tggatgtccg cttcgagggt gggggaaagc tcaggttcgg
     2221 gaatcaattt ccagttcatg tagaggcgaa ttataaaacc tgtccctttt atctctcaaa
     2281 aagttataat ccaacatcat gaaccaacca aaattgatcc ttgtcaccgg tgcaaccggt
     2341 tatgtgggcg ggcgtcttgt tcctaaactt cttgaagccg ggtatcgcgt gcgttgtctt
     2401 gtgcgagatg tgtctcgttt acaagggcgt gcctggtcga aaaaagtaga agtggtggca
     2461 ggcgatgcgc ttaatctgga tgaacttgcc tccgcgatga aagatgtgtc ggttgcgtat
     2521 tatctgatgc atggcaagca gggcggcaaa gacaatgccg aacgcgacct gaaagccgca
     2581 cgaaattttg cgcaagccgc tgaagatgaa ggcattgagc aaatcgttta tctcggtgag
     2641 ttggtagacc ccacagcgaa tctttcgccg tacttgcggt cgcgccatga aacggggttt
     2701 gtgttgcggc atggcaaagt tcctgtgaca gaaattcgcg ccggtatgat catcggctcg
     2761 gggtcggcgc tctttgaaat gattcgttat ctcaccgagc gtgaaccagt gttgacctgc
     2821 ccagcttggt tcttctctca agcacaaccg attgccattc gagatgtgct ttcctatctt
     2881 gtggatacgc tcaagacgcc agatgtcatt ggcagagtca tcgaagtggg cggacccacg
     2941 cgcctcacct acgcggacat gttgctgggg tatgccaaag agcggaatct ccgccgttgg
     3001 ctgattcgca ctcccttcta cgccccaagg ttatctgctt attgggtgca tatgctcaca
     3061 ccgattcact ggcgtgtggt cgcaccgctt attgaagggt taagcgcaga gttgatcgtc
     3121 cgcgatgaga cggcgaagaa attattcccg catattcaac cgatcgattt tcaaacagct
     3181 gttcatctcg cgcttgggcg tgttcaacgc gacaatgtgg aaaccagttg ggcagatgcg
     3241 ctcgtcacgg aagcggggga tgtccaaccg tatgtgttta ctgtagaaga tggcttgttc
     3301 attgaacgtc gacaagtggt attagagctt tctcctgaaa cagtgtttcg ttcctatacg
     3361 ggcattggtg gcgcgcgcgg ttggatgtat atggattggg cgtgggcgat ccgcgggtgg
     3421 atggataaag ccattggtgg tgtaggcttg cgccgtggac gacgtcatcc cgatgaaatc
     3481 aacaccggtg aagcgctgga tttctggcgt gtggagttgg tggaaaaatt ccgcatcatg
     3541 cgcttgcgtg ctgagatgat cgtgcccggt aaagcctggc tgcaattcga gtctacgccg
     3601 ctggaagaag aaaaagggaa gaccatattc acggtcacgg cttattttga gccatatggt
     3661 gtgcctggct ttttgtattg gtattcgatg tggcccttcc ataaatttat tttcaatggg
     3721 ctggcgcgta ggctggcttc aagggcaagg gttttggggc acgcttacta agataatgct
     3781 ctgtgagagg cacgctctcc acagagcatt ttttatagtt tggggttata gattgcatga
     3841 atatcctttt ccctctcctc ggttatatct ttggctcttt gacgttttcc atttggatta
     3901 cgcgttatgt caaacaggtg gacgtgcgtg actcgggttc tggtcacgcc acaacgacga
     3961 atacgattcg acaggcgggc tttggttggg gagcgttggt gttgatcctt gatattgcca
     4021 aagggttctt gccaacttat cttgctacta aatattctgg cgagacgtgg gtcattgccc
     4081 taactgccac cgccgctgtg atcggtcact gttggcccgt ctttgcgcaa tttcgcggtg
     4141 ggatggggct ggcaacggca gggggaagtg tccttgcggt gaatccgctg gcgtttgtga
     4201 tttgcttggg attattgatc cttcttgtct tggtggttcg tcattcagcg cgggcgagcg
     4261 tctttgctgg gattctgttt gcgccggttt tatggttgct caatattaga gacgtggcat
     4321 tttgggtggc agtgggggcg ggggcggtga tagccgtccg ctttttgatt gattggaatc
     4381 gcaagtatcg tgagttgtgg ttggatcggg agaagaaaag cgaaaaggta gaaaaggctg
     4441 agtaagacag acccatgatc ggctaagaaa gaagcgtaaa aaaaggacgg ggttaccgtc
     4501 cttttttatt ctccagttag ccacccaaga atgctctttt tgggcagggt ctcttccact
     4561 cgcggcattg ccagtaagac gaccgtgata ttgtcgtgcc cgccgcgttc attggcgagg
     4621 ttgaccaagg tctctgccgc ggtttttagg tcatgcttgc cgcggatgat gtctttgatt
     4681 tcatcgtccc aaacgaggtc ggtgagccca tcagtacaga tcagtatggt gtcaccgggt
     4741 tgaaggtgaa acccttgatt gttaagcgac tcttcttcgg tctcattgtt atcgaggcgc
     4801 aggcggaagt ccacttcggg caggcgaatg ccgccaagat gccgacgaat gacatgcaca
     4861 ttggggtggt cgcgcatttg gtcggcagtg ataatgcctt tttcatacgc ttcctgcacc
     4921 caagtatggt cgatggtgat ttgctgaatg tattttccgc gcaaaagata aatgcgcgag
     4981 tcgcctacgt atgcggtgta gagtttgttc tcaacaatcc acacacaggc gcaagtagag
     5041 cccatgcctt gctcttctgc cttgcccgcg gagtgggtgg caattgcctg gctggcatcg
     5101 tgaatggcat tttcgaggat tttgataggt ttgcgggcgt tgctctcagc aatatgcatg
     5161 ctgatgtagt taactgccaa ttccgcggca acttcacccg cgcggtgtcc gccaatacca
     5221 tccgcaacaa cggcaaacgt tgaagggcgt gggtcatctt tgctgagctg gaacgacgag
     5281 acggcatacc ggtcctcatt gttcttgcca gagatgcccg catggctaag agcggctatg
     5341 tgcaaatgag caagtgtggt acggatcatt agtcttcagg agtcacaggg gtgattcttg
     5401 gagttctcgg tggcggggga gtccgcaaca taaagcggta ctttaattga ccaaagttta
     5461 ccatatcccc atgcagtaac cggtagccct cacgggggat aggctcgtaa ttgacccagg
     5521 tgccggcgag cgactgggca tccatcagta gataaccgcc atcttctgtg gcgcgaatcc
     5581 gcgcatggac agacgaaata gaggggtcat ccaaaatttg cgagcattgc acagggtcgg
     5641 cgccaaaaac gatctctgca tcgtttaatt ggataggcgg gacagccgcc acttgtccat
     5701 cggcttggag gcggacgaga gaagccaacg cctcattctc caacgttttg gctgctttcg
     5761 ccttcgtgga cttggtgcgt ggctttttag ttttttcttt caccgccacc ggagccggtt
     5821 ctgtaaacgc ttcaagagac tgggtcaatg ggtcgttttg aacgcggcgt tcttcttgca
     5881 taacccgcaa cgaacggatg cgtaaccgtc cactgagcag gatggcgaat aagacaagcc
     5941 cagcgagaat gatcgcgccg aaggttaatg gcatgcgata ttttgcaagg aaggcagcag
     6001 gtccgctggg cggtttgatg acggttacca ctaccggcaa aggcatgctg gtcttactga
     6061 gacccagaga gtccacggct tcaacggtca attgatgctc accgctaatt gtgtaaggct
     6121 tcaagtccca ggtaaatttt gtaaatggct cggctgtgtt ttcatccacg gcaatgccat
     6181 ccacgatcag agtggtgcga gcaagcgggc gatcaaaccc atctggaaat tcgatgatga
     6241 tttcgatctc ttgtgtttct ggcaaaagga cttcggtgtt gaaggggtct tcttcaggcg
     6301 cttggcgcgt aatctgtatc gacggcgcga ccgggaaggc attgggtggt tggatgttga
     6361 gggtgaaagg aagtgtctct gaattcagcg gaccaacaga aggcagattt acttgaaccg
     6421 ctacagtatg gttgccagag gtgttgacac gcgaggtgta agagaaattg taaattctac
     6481 gcaacggaga gaaatattct tcagggtccg ggaagcgttc ctcgcctgag aattggaaca
     6541 tggtgccgcc ggtttggata gcaaggttgt tgaagacggc agcactggtg gttgtgaagg
     6601 ttgtgtttgc gtccacatac cagatgaaga cgcgaatttt attttcaagg gcgcgttgga
     6661 tcagcggctc aaccgatgcg gcgaggttgg ggtccgtcat ttgcggtgtg atgaatagaa
     6721 tggcacgctt cacaccgatg cgtggggttt gggcgctgac cacatctagc gcgctgacca
     6781 gagattgaat gttgggagta gcggtgcgca ggtctggctg gaagcctttt aagccgacta
     6841 cgaaatctgc tggcggggca tggttgatga ccggacccgc ttgcgagaca aggcttaggt
     6901 catcgggcag gtctgctggg cgggtttgtg cccactgctc aatgatttgc tccacccgct
     6961 gataacggga aatcccattt gcattttgtg cccctaaggc ttctccctga ttgacggcaa
     7021 caactaactg aagagggatc gctatttcat tgaacgaatc ggctgggatg ggctgcccgt
     7081 tctcgataag ggtaaccgct tccggcttga gtcctgaggc gaagatgccg ttggcatctg
     7141 tcacgtccac gaatccagtt acagatggga agttggatgc atcgacgtta taaatagtaa
     7201 cggacgcggt gaccgacccc tgcgcttggg cagggacgat caccacgccg aacaaaaggc
     7261 taattgat
//
LOCUS       DAOQJE010000050         7259 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_5673_length_7259_cov_6.073154, whole genome shotgun sequence.
ACCESSION   DAOQJE010000050 DAOQJE010000000
VERSION     DAOQJE010000050.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 7259)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7259)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7259
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5673_length_7259_cov_6.073154"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..513
                     /locus_tag="PLT08_02270"
     CDS             <1..513
                     /locus_tag="PLT08_02270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE03316.1"
                     /translation="IDAVLIGQTAEVVGKNSGVPNYWVVKRLNGSGTCWLWGQYATVS
                     GNTANLPEYPVPPTPTPSPTPTFTPTPTLAPPAPVNNVSVVKNCIPLVFPTTYTYTGI
                     LNWEDKSNNENGFNIYFNGALFASIPANSNSYPLPALPFAAGTPVKWGVEAFNSAGKA
                     AIKDVIFACP"
     gene            586..1149
                     /locus_tag="PLT08_02275"
     CDS             586..1149
                     /locus_tag="PLT08_02275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE03317.1"
                     /translation="MSTFHIPQTILVPNDANQRALEFANAVTQTVNYRDSNQSIQEKI
                     RDDHFVSKLGEEAVRILFQSRECLVEGPDYGVYEARRKSWTADLKINGLDVAVKTQRR
                     SAANRYGLSWTFQDSPERHDPILDMPDAWVCLVVFEDLKKEPECLVYPLRKIKQLIFE
                     APRLSKLVGKKQAVYLETLIKNGVIEK"
     gene            complement(1213..2544)
                     /gene="ahcY"
                     /locus_tag="PLT08_02280"
     CDS             complement(1213..2544)
                     /gene="ahcY"
                     /locus_tag="PLT08_02280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004013 - adenosylhomocysteinase activity
                     [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylhomocysteinase"
                     /protein_id="HNE03318.1"
                     /translation="MSNYDIKDLQLAEGGRRRIDWAEREMPVLRSIRERFKKEKPLKG
                     MRLSCCLHVTTETANLMITLQEGGADIVLTASNPLSTQDDVAAALVNNYEIPVFAIKG
                     EDKVTYFKHIRAALEHKPHMTQDDGADLVSSLFFIEMGRFEKLEPSLASWAKGLSEEE
                     RKQMLKNVIGGTEETTTGVIRLKAMEADGVLKFPVIAVNDALTKHMFDNRYGTGQSTI
                     DGIIRATNVLLAGKTFVVAGYGWCGRGLASRARGMGAHVIVTETDPMKALEAVMDGFQ
                     VMSMLDAAKIGDIFCTVTGDINVLDKHHFEVMKDGALVSNSGHFNVEINIPALAAMSK
                     GEPNLVRPFVEQYTTKDGRKINILGEGRLINLASAEGHPASVMDMSFANQALSAEYMA
                     KNADKLEKKVYSVPAEIDNEIARLKLEAMGVKIDKLTDEQLHYLNSWEEGT"
     gene            complement(2741..3697)
                     /locus_tag="PLT08_02285"
     CDS             complement(2741..3697)
                     /locus_tag="PLT08_02285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase family protein"
                     /protein_id="HNE03319.1"
                     /translation="MDILLTGSVAYDYLMTFPGHFKEQILPERLESISLSFLVDSMSK
                     QRGGIAPNIAYTMALLGQKPRVMATVGEDFGDYRAWLEAKGVDTNLMKVVPGVFTASF
                     FATTDHASAQIASFYPGAMGYSATQSLKELDKKPDLVIVSPSSPDAMMKFPAEARELG
                     IPYLYDPSQQVLRLEGPELARDMEGAYFLFCNDYEFGLISKKTGWSLDQILNHVKVLV
                     ITRGKDGADLYSDGTSVHIPTVPEDEIVDPTGVGDAFRGGFLAGYSHGFDWKLCGEIG
                     SLSAVYCLEQRGTQNHSYTREDFVKRFRKHFDDGGRLNVLLK"
     gene            complement(3834..4985)
                     /locus_tag="PLT08_02290"
     CDS             complement(3834..4985)
                     /locus_tag="PLT08_02290"
                     /inference="COORDINATES: protein motif:HMM:NF024808.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0019808 - polyamine binding [Evidence
                     IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015846 - polyamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     substrate-binding protein"
                     /protein_id="HNE03320.1"
                     /translation="MNTHHKIHRWFRLILSSVLLIAVTACAAGSAPASTDELVTANGF
                     VCPEPSPRVEFESDTINIFTWTEYVPADMIDCFGMVYDITVNVDYFSSNEELYSKVSL
                     GEDVNPYDVIHPSDYMIAVLMREGLLQELDPARLPNLANLDSGLVTAYGEATSYVVPY
                     QMGTQAIIYNSDTVETPPTSWADLWNPEYEGRIVSVDDSRVVIGAALLTLGYDVNDTD
                     PDHLEEAKQKLLELMPNIRVFDSDSPKTPLVAGDVDLGIVWNGEAFLTASEAPQFKYV
                     FPEEGSIIFYDGMGIPPSATHADAAYAWFNYMLQGDVNWLTLVDYPYTNPNKAALDYA
                     KENQPDIYEAYISSSITNTPADEFAKGHDVEDIGEALLIYDEIWTEIKQ"
     gene            5042..>7259
                     /locus_tag="PLT08_02295"
     CDS             5042..>7259
                     /locus_tag="PLT08_02295"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNE03321.1"
                     /translation="MNTTLEIINAPLDALILYQYKNGMKKLRLLLGLWLATSLVACST
                     LKNFPTIPPGWTVTPSTSPTPEATFTPTITPTPLPVARVSEGDKAFFNGDFDAALLHY
                     QVAIQDSPDPLVRAAAEWGQAKIYYAQGRYNETLTSLQTLISEYPQAPQHPQAYFLQG
                     FVYYKLENYPAAADSWQTYLVLRPGVLDAYVQELRGDALFNAKDFAGALASYTTAITA
                     PSLGDDTILDLKVASVQTQLGNYEEAIALYDGIIGRATSDYTKAQALYEAGTVNLTQG
                     QTAEANERFRYAIENYPLSYYSYLSLVALLDAGGTVSDLDRGLVDYFSGQYAVGIAAF
                     DRYLQSNPADNDGTAYYYRALCKNALGQYDEAIADYQNFISNFSAHPRWGDAWGEKAF
                     IEWAQKGDYTTGAQTLLEFINLAPTSPQAAEYLMSAARIYERDAQYDKAIETWSRVVI
                     EYPGSQQASYAAFLSGIVEYRRGNHQASLDLFKRSLALSVSVEDKSRALLWIGKANQI
                     LGNQGPAEDAWREGQNLNPGGYYSERARDLLVGQAPFATGSAQNYQVDYARERADADT
                     WMRLTFNLGNDVDLSNLGSLASDARIIRGKEYWDLGMLDEARLEFEDVRSELETLNDA
                     VGSYRLANYLVDLGMYRSAVFAARQVLTIAGLDEHTESMMAPAYFSHIRYGLYYSDLV
                     LPTSQQEGFDPLFIFSVIRQESLFEGFVRSNAGAHGLMQIIAPTGAQIASELGKPLN"
BASE COUNT         1957 a         1783 c         1697 g         1822 t
ORIGIN      
        1 atcgatgctg tgcttattgg gcaaacggca gaagttgttg gcaaaaattc cggcgtaccg
       61 aattattggg tggtcaaacg cctgaacggg tctggaactt gttggctctg gggacaatat
      121 gccaccgtta gcggtaatac agcgaatttg ccggaatatc cggttccgcc cacgccgaca
      181 ccttctccaa ctccgacatt tacacctaca ccaacacttg caccaccagc gccagtaaat
      241 aatgtttccg ttgtaaagaa ttgtattcca ttggtatttc caacaacgta cacctatact
      301 ggaatactta attgggaaga taaatcgaat aacgagaacg ggttcaatat ctatttcaat
      361 ggtgctttgt tcgccagtat tccagcgaat agcaacagtt atcctttgcc ggcgttacct
      421 tttgctgctg ggactcctgt aaaatggggc gtggaagcct tcaactctgc aggcaaggct
      481 gcaattaaag atgtaatatt cgcctgccca tagaaagttg ataagtatta aaaagccttg
      541 cagaagtttt tctgtaaggc tttttatttt gtataatcag aaacgatgtc aacatttcat
      601 attccgcaaa ctattcttgt gccaaatgat gccaatcagc gagcattaga gttcgccaac
      661 gctgtcacac aaactgtcaa ttatagggat agcaatcaat cgattcaaga aaaaatacga
      721 gatgaccatt ttgtctctaa acttggcgaa gaagcagtgc ggattctctt tcagagtagg
      781 gaatgcttgg tggaaggacc agattacggt gtgtatgagg cgaggcgtaa atcctggacg
      841 gcagacttga aaatcaacgg attggatgtg gcggtcaaaa cccaacgtcg ctccgctgcg
      901 aatcgctatg gactttcatg gacatttcaa gactcgcctg aacggcatga tccaatttta
      961 gatatgccag atgcatgggt atgtttagta gtttttgaag atctaaaaaa agaaccagag
     1021 tgtctggtat atccactgag gaagataaag caactgattt ttgaagcgcc gcggctctca
     1081 aagcttgttg gaaaaaaaca agccgtttat ttagaaacac ttatcaaaaa cggtgtaatt
     1141 gaaaaataaa acagtcccac gaaattcgtg ggactgtaat cattaatcca aaatcgttaa
     1201 tcgtaaatcg acttaggtgc cttcttccca agagttgagg tagtggagct gttcgtcggt
     1261 gagtttgtcg atcttgacgc ccatcgcttc gagcttgagg cgagcaatct cgttgtcgat
     1321 ctcggcagga acagaataca ccttcttctc gagtttatcg gcattcttcg ccatgtattc
     1381 agcagagaga gcctgattcg cgaaggacat atccataacc gaagcggggt gaccttcagc
     1441 agaagcaagg ttgatgaggc gaccttcgcc gaggatgttg atcttgcgac cgtctttggt
     1501 ggtgtactgt tccacaaacg ggcggacaag gttgggctcg cccttgctca tggcagccaa
     1561 agcagggatg ttaatttcca cattgaagtg accagagttg gaaaccaaag cgccgtcttt
     1621 catcacttca aagtgatgct tatcaagcac attgatatca ccggtaacgg tgcagaagat
     1681 atcgccaatc tttgctgcat ccagcataga cataacctgg aagccgtcca tgactgcttc
     1741 gagcgccttc atcgggtcgg tttcggtgac gattacatga gcacccatac cgcgagcgcg
     1801 ggaagcgaga ccacgtccac accagccata gccagcgaca acaaaggtct taccagccaa
     1861 caaaacgttg gtggcgcgga tgataccgtc aatggtggac tgaccggtgc cgtagcggtt
     1921 atcgaacatg tgcttggtga gcgcgtcgtt cacagcaatg accgggaact taagcacgcc
     1981 atcggcttcc atcgccttca agcggatcac gccggtggtg gtttcttcag taccaccgat
     2041 aacgttcttg agcatctgct tgcgttcttc ttcgctcaaa cccttcgccc aagaagcgag
     2101 agaaggttcg agtttctcga aacgtcccat ttcgatgaag aaaagggagg aaacgaggtc
     2161 ggcgccatcg tcctgggtca tgtggggttt gtgttccaat gcagcgcgga tgtgcttgaa
     2221 gtaagtgacc ttatcttcgc ccttgatggc aaagacaggg atttcatagt tgttgaccaa
     2281 cgccgcagcc acatcatctt gagtggaaag cggattggag gcagtgagga cgatgtccgc
     2341 gccgccttcc tgaagggtga tcatcaggtt tgcagtttcg gtggtcacat gcaaacagca
     2401 agacaggcgc atacccttga ggggcttttc cttcttgaag cgttcgcgga tggaacgcaa
     2461 cacaggcatt tcgcgttctg cccaatcaat gcgacggcgt ccgccctctg ctaattgaag
     2521 gtctttaata tcgtagttgc tcataaatac tccttaagtc tttatacaag gaatgtgatt
     2581 tggaaaccca tttgtgtccg cataaaaggg ttcaaatcac ggactaataa tttaatttag
     2641 gttggtctca acagttgaca ttgctttcaa gcggagcaat gcgtatgtac ggcaacattc
     2701 tgcgaacacc gccagcttaa ccaacaaatc taaatagatt ttactttaat aagacgttca
     2761 gtctgccgcc atcatcgaaa tgcttgcgga aacgtttgac gaaatcttcg cgtgtataag
     2821 aatggttctg ggtgccgcgt tgttcaaggc agtagaccgc agaaagggag ccgatctcac
     2881 cacacaactt ccaatcaaag ccgtgagaat acccggcgag gaacccgcca cggaaggcat
     2941 cccccacacc tgtggggtct acaatttcat cttcaggcac ggtgggaatg tgaaccgaag
     3001 tgccatctga gtagaggtct gcgccgtctt tgccacgggt aatcaccaag accttaacat
     3061 gattgaggat ctgatcgagg ctccagccgg ttttcttgga gatcaagcca aattcgtagt
     3121 cattacagaa caagaaatat gccccttcca tatcgcgagc aagttctggt ccttcaaggc
     3181 gcaagacttg ctggctgggg tcatacaaat atggaatacc caattcacgt gcctcggcag
     3241 ggaatttcat catcgcatct ggcgaactgg gagagacaat aacgaggtct ggttttttat
     3301 caagctcttt gagcgattga gtcgcagagt agcccatggc tccggggtag aacgaagcaa
     3361 tctgagcgga ggcatgatcc gttgtcgcga aaaacgaggc ggtaaacacg cccggaacca
     3421 ccttcatcag gttcgtatcc acgccttttg cttcgagcca ggcgcgataa tcgccaaagt
     3481 cttcacctac agttgccatc acgcgcggct tttgtcccag caacgccatg gtgtaggcaa
     3541 tgtttggggc aatgccaccg cgctgtttgg acatggaatc aaccaagaac gaaaggctga
     3601 tcgactccaa acgttcgggg aggatttgtt ccttgaaatg acccggaaag gtcatcaaat
     3661 aatcataggc tacagagcca gtaagaagta tatccatgct gtttccttct cgcagtaaat
     3721 ggcgcactcg gttgagtgcg ccataagata tcgaacgggc aaaagtttat cacggggttt
     3781 tatgaatgta aagtttttga gcttacaaaa ataacccgtg caattcgcct actttattgc
     3841 ttaatttccg tccaaatttc gtcgtatatc agcaaggctt cgcctatatc ctcaacatca
     3901 tgcccttttg caaactcatc tgccggggtg ttggtgatgg atgacgagat ataagcttcg
     3961 taaatgtctg gctgattttc tttggcataa tctaacgcag ctttgttggg gttggtgtac
     4021 ggataatcga ccaaggtcag ccaattgaca tcgccctgta acatatagtt gaaccaggca
     4081 tatgctgcat cggcatgagt cgctgaaggc ggaatcccca taccatcata aaagatgatc
     4141 gacccttctt ctgggaaaac gtatttgaac tgcggagctt cagaagccgt caggaacgct
     4201 tcaccattcc aaacaattcc caaatctaca tcgcctgcca ccagtggagt tttggggcta
     4261 tcgctatcga acacacgaat gttcggcata agttccaaca acttctgttt agcttcttct
     4321 aaatggtctg ggtcggtatc gttgacatcg taacccaaag tcaacaaagc ggcaccaatc
     4381 accactcggc tgtcatccac agacacgatg cgaccttcat attcagggtt ccaaaggtca
     4441 gcccaagaag ttggcggggt ctccacagtg tcactgttat agataatcgc ctgtgtcccc
     4501 atctgatacg gcacaacata actagtcgct tcgccatatg ccgttacaag acccgagtca
     4561 aggtttgcca aattaggcaa acgcgcagga tccagttctt gcagtagccc ttcacgcatc
     4621 aaaacggcga tcatgtaatc actggggtga attacatcat aagggtttac atcttcgcca
     4681 agcgaaactt tcgaatacag ttcttcattt gaagaaaaat aatcaacgtt aacggtaatg
     4741 tcatacacca tgccaaaaca atcgatcata tcggctggca catactccgt ccaagtgaat
     4801 atattaatcg tatcactttc aaattccacg cgtggactcg gctctgggca aacaaagcca
     4861 ttggcagtga ccaattcatc tgtcgaggcg ggggcagacc cagcagcgca ggctgtcacg
     4921 gcgatcagca gcacgctcga caaaatcaac cgaaaccaac gatggatttt gtgatgtgtg
     4981 ttcataattc tccttagggt tataaaataa tgtaacaaag agattatagt gcaagctaaa
     5041 cgtgaataca acattagaaa tcataaatgc cccgcttgac gcactcatcc tttaccagta
     5101 taaaaacggg atgaaaaaac tgcgcctcct tctcggactt tggcttgcca catctctcgt
     5161 agcttgttct acgctcaaaa atttccctac catccccccc ggctggacgg ttactcccag
     5221 cacatccccc actcccgaag cgacctttac tcccaccatt acgcctacgc ctttacccgt
     5281 tgcccgagtc agcgaagggg ataaagcctt cttcaacggt gattttgacg cagccttatt
     5341 acactatcaa gttgccatac aagattcacc agaccccttg gtgcgtgccg cagcagaatg
     5401 gggtcaagcc aaaatatatt acgctcaagg gcgctacaac gaaaccctca catcccttca
     5461 aacccttatt agtgaatatc ctcaagctcc tcaacaccca caagcttatt ttctacaagg
     5521 ctttgtctat tacaaacttg aaaactaccc tgcggcggct gattcctggc aaacatattt
     5581 agtcttgcgc ccaggcgtat tagatgccta cgtacaagaa ctgcgaggtg atgccctctt
     5641 caatgcaaaa gattttgccg gagcgttggc gtcttacacc actgcaatca ctgccccatc
     5701 tctcggcgat gacaccatct tagacttaaa agtcgcatct gtacaaacac aactcggcaa
     5761 ttatgaagaa gcaattgcgt tatacgacgg aatcattggg cgagccacca gtgactacac
     5821 caaggctcaa gcgttatacg aagctgggac ggtgaatctc actcaaggtc agaccgccga
     5881 ggcgaatgaa cgtttccgat acgctataga aaattatccg ctttcctatt attcctacct
     5941 tagccttgtc gccctacttg atgctggtgg aacagtcagt gacctagacc gtgggctcgt
     6001 agattatttt tcaggtcaat acgcagtagg catcgccgca ttcgatcgct acttacaaag
     6061 taaccctgca gataatgacg gtacggcata ttattaccgt gcgctttgta aaaacgcatt
     6121 ggggcagtac gatgaagcga tagcagatta tcagaacttc atttcgaatt tctctgcaca
     6181 tccacgctgg ggagatgcat ggggtgaaaa ggcgtttatc gaatgggcgc aaaaaggaga
     6241 ttacaccacc ggcgctcaaa cgctcttgga gtttatcaac ctcgccccta cttccccaca
     6301 agcagctgag tatcttatga gtgccgcacg catttacgaa cgagatgccc agtatgataa
     6361 agcgattgaa acatggtctc gtgttgtcat tgaataccct ggttcgcaac aagcttcata
     6421 tgcagcgttt ttatctggca tcgtggaata ccggcgcggc aatcatcaag catcgttaga
     6481 cctttttaaa cgcagcctgg cactttcagt ttctgttgaa gataaaagcc gtgccctgtt
     6541 atggatcggc aaagccaatc aaattttagg aaatcaaggt ccagctgaag acgcgtggcg
     6601 tgaaggacaa aacctaaacc ctggcggcta ttacagtgaa cgtgcgcgag acctattggt
     6661 agggcaagct ccatttgcaa caggcagcgc ccaaaattac caagtggact acgccagaga
     6721 gcgagccgat gccgatacct ggatgcggtt aacattcaac cttggcaacg atgtagactt
     6781 gagcaacctc ggttcgttgg cttcggacgc acgcattatc cgtggcaaag aatattggga
     6841 tttgggcatg cttgacgaag ctcggcttga gtttgaagat gtacgcagtg aacttgaaac
     6901 gctcaacgat gccgttggca gttaccgact cgccaactac ttagtcgatt taggcatgta
     6961 tcgctctgct gtttttgccg ctcgccaagt attgaccatc gcgggcttgg atgaacacac
     7021 tgagtctatg atggcgcccg cctatttcag ccacatccgc tacgggttgt attattcaga
     7081 tttagtcctc cctacttctc aacaagaagg gtttgaccca ttatttatat ttagcgtaat
     7141 tcggcaagaa agtttatttg aaggctttgt ccgctcaaat gcaggcgccc atggacttat
     7201 gcaaattatc gcccctaccg gagcgcaaat cgcctcagaa ctcggaaagc ctttgaact
//
LOCUS       DAOQJE010000051         7231 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_5704_length_7231_cov_3.997492, whole genome shotgun sequence.
ACCESSION   DAOQJE010000051 DAOQJE010000000
VERSION     DAOQJE010000051.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 7231)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7231)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7231
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5704_length_7231_cov_3.997492"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..987)
                     /locus_tag="PLT08_02300"
     CDS             complement(<1..987)
                     /locus_tag="PLT08_02300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="cache domain-containing protein"
                     /protein_id="HNE03322.1"
                     /translation="MKRILPGWRGLTQLFAVTVLPLTLLLLFIAFGGVNMHQQDMRTL
                     VGERDERAVQSAAAALQSELHHRMATVSSMAVLASEDISFKDIFATTTDLAKDFNGGI
                     AFLNTDGHLIEKTGNDRFWGWVSQNSYSIKLASSSSPQLVFSDPTLDPNSNQLFVIIS
                     AYSDSRDVIVAGAFSPETLASETLTPTYPAGSHVTIYLLDNSRRILFVSGALERESLD
                     ADHPGVAEALAGRSGVLYVKKEATEHVVAYSPIETAGWALITEEEWEMVISPSLQLTQ
                     MAPLVMVPAFILALIAIWFGAKQIVQPLQKLESKAAALAWGDFEAIKESVGGI"
     gene            complement(984..1673)
                     /locus_tag="PLT08_02305"
     CDS             complement(984..1673)
                     /locus_tag="PLT08_02305"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HNE03323.1"
                     /translation="MPKSSSKSKTKIRILLADDHHIVRAGVRQLLESATDLQVIAEAG
                     DGEEAQVLIQKHKPDVAVLDIQMPKASGIEVTRWVRAHLPEVGVLILTAYNDDPYVMA
                     VLQAGANGYVLKTGQADELIQAVRDVYEGKSALDPSITSKLMSTIFKGPEKKIVEPLT
                     DRELDVLRLAAKGFTNKSIGVQLNISDRTVQGHLAHIFAKLQSNSRTEAVMRGVALGL
                     ISQSSGNIPEE"
     gene            complement(1618..2388)
                     /locus_tag="PLT08_02310"
     CDS             complement(1618..2388)
                     /locus_tag="PLT08_02310"
                     /inference="COORDINATES: protein motif:HMM:NF021384.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2085 domain-containing protein"
                     /protein_id="HNE03324.1"
                     /translation="MSTTTLNAPKITNAFQWIGTHWFAVFLTIYGVWVFVPFLAPVFM
                     QIGWTGAGRAVYFIYSFFCHQLPERSLFWFGEKTMYSLGEIQAVWQNTSNPMILRQFI
                     GNEPMGWKVAWSDRMISFYTSVWLFAALWYPFRHTIKTLSWWGFVLLLLPMALDGGTH
                     MVSDFAGIGNGFRDTNAWLAVLTNNTFPATFYAGDALGSFNSLMRFLTGLLAGLGLVW
                     LAFPFINQSQVYNQQLDTLSHAKVIEQIKNQNTHSPGG"
     gene            complement(2398..2976)
                     /locus_tag="PLT08_02315"
     CDS             complement(2398..2976)
                     /locus_tag="PLT08_02315"
                     /inference="COORDINATES: protein motif:HMM:NF022747.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3105 domain-containing protein"
                     /protein_id="HNE03325.1"
                     /translation="MNNKQRQQYIQQRNRKQKLLAGIIWGGTGLAVLVILGVMVWQGI
                     KPAAGEAVAIMKSDPHLPTDSDPGQYNSDPPTSGLHYATEAQAGFYEENIYTYPAAYL
                     VHNLEHGYVIIWYNCDLLNETGCAELKSQIRTVINDLGGVKLIAYPWNSIDVPVALTS
                     WGRIQRFEIFDMDLAKAFYRANLNRAPEPNAP"
     gene            complement(3040..3441)
                     /locus_tag="PLT08_02320"
     CDS             complement(3040..3441)
                     /locus_tag="PLT08_02320"
                     /inference="COORDINATES: protein motif:HMM:NF024515.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FixH family protein"
                     /protein_id="HNE03326.1"
                     /translation="MRKMFFISMLVLLAGVLAACGSSAQPAAPVESGSSSPVNIQIET
                     NPNPAMMGNMELILTITDGDGKPIEGATVDVSADHTDMTGMGMSGLATEQGEGRYSIN
                     ANFSMSGNWKITVYVRKDGLDYKEDIEFKVQ"
     gene            complement(3464..4450)
                     /locus_tag="PLT08_02325"
     CDS             complement(3464..4450)
                     /locus_tag="PLT08_02325"
                     /inference="COORDINATES: protein motif:HMM:NF012612.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper oxidase"
                     /protein_id="HNE03327.1"
                     /translation="MNNDNSGMADGTPTPGSMMMENPEAAHMMEPITAPNVQPATEAD
                     GGQPLEYRLEDGVKVFELTTKAVQWEILDGVTVTAFTYNGTVPGPMIRVTEGDQVRVI
                     VKNELPDPTTIHWHGVEVPNAMDGVPGVTQDPIQPGETFTYEFTAKPAGTFMYHSHYE
                     GDVQVGAGLYAPFIIDPKEPDANPPVVDKTLMISEWRMTDGQTYAAMPMSGMEPNYFT
                     INGKSFPATETITVKKGDLVRLRFIAIGQFIHPMHLHGVPFRIVATDGHPVPEVAQLT
                     KDTVSVAPGERYDIEFVATETGQWMLHCHILHHTTNDNVEPGGLMLMIEVVE"
     gene            complement(4650..5003)
                     /locus_tag="PLT08_02330"
     CDS             complement(4650..5003)
                     /locus_tag="PLT08_02330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE03328.1"
                     /translation="MTTSSFLIVDLPCDMALQAAKKKLLQGGLRALQTFDLHTARHTQ
                     QDCPCPHHGTADCDCQMVVLMVYGESAEPVPLILHGSDGQTRFSIADDPSQRIDKKLV
                     ASIKEALDIRAAVSV"
     gene            complement(5160..6272)
                     /locus_tag="PLT08_02335"
     CDS             complement(5160..6272)
                     /locus_tag="PLT08_02335"
                     /inference="COORDINATES: protein motif:HMM:NF021778.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-propeller fold lactonase family protein"
                     /protein_id="HNE03329.1"
                     /translation="MNKFLIWKIMPALIIATVVLSACGAPAKPEMTEQPAQTQPDQPV
                     AGLDGTVWVADEEGNSITVINAATNQVLTTLTGVEGAHNLQVAPDGKTVWAVSGHDSL
                     AIKIDAVTFEVHGVVPTGMMPAHIVLTPDGKKAYATNGGDNTVTVIDVEAMSAIATIP
                     VGEYPHGLRVSPDGMWVYVANAKSTTLSVIDTAQDVKVADIEVGEHPVQVGFSPDGEF
                     VYFSLNGENALGKVDVAAQELVGKVDVGGGPIQVFVTPNNKFILTANQGTKDTPSTTV
                     SVVDVATFQVIATIETGQGAHGVVIDPSSQYAYISNIYEDTVSVIDLEKMAVVATVPT
                     GMAPNGISFSPLPAPGNAPAEIQLEIPEGGMNMPMP"
     gene            complement(6398..6604)
                     /locus_tag="PLT08_02340"
     CDS             complement(6398..6604)
                     /locus_tag="PLT08_02340"
                     /inference="COORDINATES: protein motif:HMM:NF016811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YHS domain-containing protein"
                     /protein_id="HNE03330.1"
                     /translation="MNSFNMRKFYPTGQEFEIDPVCEMKVDPKNPPFQVVHKGRTYYF
                     CSEVCKHLFERVPDQYVRIEEDKV"
     gene            6851..>7231
                     /locus_tag="PLT08_02345"
     CDS             6851..>7231
                     /locus_tag="PLT08_02345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE03331.1"
                     /translation="MKRFFIPFVAFLLGSALIASFYIGILTWAQGWDYASSQFIRDRW
                     YVIPIILGFGIQAALYSILRFRLFIPVTSTGHAGSMIGASGGTSATAMVACCIHHVTD
                     VLPILGLSAAASFLTRYQRPFMLIG"
BASE COUNT         1660 a         1955 c         1835 g         1781 t
ORIGIN      
        1 tatgccgcca actgactcct ttatggcttc gaaatccccc catgccagcg ccgccgcctt
       61 ggactcaagt ttttgcaggg gctggacgat ctgtttggcg ccgaaccaga tggcaatcaa
      121 tgccaggatg aaggcaggca ccattaccag cggagccatc tgggtcaatt gcagggaggg
      181 gctgatcacc atttcccatt cctcctccgt gatcaatgcc cagcctgcag tttcaatggg
      241 gctataggcg actacatgtt ccgtcgcttc ctttttcaca tacagaacgc cactcctgcc
      301 tgccagagct tccgccactc caggatggtc cgcatccagg ctttcgcgtt cgagcgcacc
      361 gctgacgaac aaaatacggc gggaattatc caatagatag atggtcacgt gactgcccgc
      421 cgggtaggtg ggtgtcaggg tttcggacgc gagtgtctcg ggtgaaaacg cgccggcgac
      481 aatcacatcg cgggagtcag aataggctga gatgatgaca aagagttggt tggagtttgg
      541 atccagggtc gggtcggaga aaacaagttg agggctggat gaagaagcga gtttgatcga
      601 gtaggagttt tgggataccc atccccaaaa tctgtcattc cccgttttct cgattaagtg
      661 cccatccgta tttagaaagg caatcccccc gttgaaatcc tttgccaggt cggtggtggt
      721 ggcaaatata tccttgaagg aaatatcctc ggatgccagc accgccatgc tcgaaacagt
      781 cgccatgcga tgatgaagtt cggattgcag cgctgcggcg gcggactgca cggcgcgttc
      841 gtcacgttcc ccgaccaggg tgcgcatgtc ctgctggtgc atgttgaccc cgccgaacgc
      901 aatgaacaat aacaaaagcg tcagcggcag aaccgtgacg gcaaagagtt gcgtcagacc
      961 gcgccagcct ggcaggattc gtttcattct tctggaatat tgcccgagct ttgtgagatc
     1021 aaccccagcg ccaccccgcg catgacagcc tcggtgcggc tgttggattg cagtttggcg
     1081 aagatgtgcg caagatgtcc ctgcacggtg cggtcgctga tattcagttg cacaccgatg
     1141 gatttgttgg tgaacccctt cgccgcgagt cgcagcacat ccagttcacg gtcagttaat
     1201 ggttcgacga tctttttttc cggtcctttg aagatggtgg acatcaactt gctggtgatg
     1261 gatggatcga gtgcagactt accttcatag acatcacgca ccgcctggat cagctcatcc
     1321 gcctgcccag ttttgaggac atagccattt gcacctgcct gcaaaaccgc catcacgtag
     1381 ggatcatcgt tataagcggt caggattaac acgcccactt cgggcagatg ggcacgcacc
     1441 cagcgtgtga cttcaatacc gctggctttg ggcatctgaa tatcaagcac ggcaacatcc
     1501 ggcttgtgtt tttggattaa gacctgcgcc tcctccccgt ctcctgcctc ggcaatgacc
     1561 tggaggtcgg tcgcactctc aagcagttgt cgcacccctg cacggacgat atggtggtca
     1621 tccgccagga gaatgcgtat tttggttttt gatttgctcg atgactttgg catggcttaa
     1681 tgtatccaat tgctgattat aaacttgtga ttgattaatg aacgggaagg caagccagac
     1741 aagacccagc cccgccaaaa gcccggtaag gaagcgcatg agcgagttga acgatcccag
     1801 cgcatcaccg gcgtaaaacg ttgcaggaaa ggtgttgttc gtcaacactg ccagccatgc
     1861 atttgtatca cgaaatccat ttccgatacc ggcaaaatcg ctgaccatgt gtgttccacc
     1921 atccagcgcc atcggcagca ggagcagaac aaatccccac caactcaaag tctttatcgt
     1981 gtgtcggaag ggataccaca atgccgcaaa aagccagacg ctggtataga acgagatcat
     2041 gcgatccgac cacgccacct tccagcccat cggttcgttg ccgatgaatt ggcgcaggat
     2101 catgggattg gatgtgttct gccagaccgc ctggatttcg cccagggaat acatggtctt
     2161 ttcgccgaac caaaagaggg agcgttccgg gagttggtgg cagaagaacg agtaaatgaa
     2221 gtagaccgcc ctgcctgcac ccgtccagcc gatctgcatg aaaaccggcg caaggaaggg
     2281 aacaaatacc caaacaccat agatggtcag aaaaacggca aaccaatgag tgcctatcca
     2341 ctgaaaggca ttcgttattt taggggcatt caaggtggtt gttgacattt tattccctca
     2401 tggggcgttt ggttcagggg cgcgattgag gttggcgcgg taaaaagctt tggctaaatc
     2461 catgtcgaag atctcgaacc tttgaatccg cccccaggag gttaatgcga ccggaacatc
     2521 aatggaattc cagggatagg caatcagctt cactccaccc aggtcgttaa tgaccgtgcg
     2581 gatctgtgat ttcaactccg cgcatcccgt ctcattcagc aggtcgcagt tgtaccagat
     2641 gatcacatac ccgtgctcca ggttatggac aagataagca gcaggatatg tataaatatt
     2701 ctcttcataa aaccccgcct gggcttcggt tgcataatgc aatccggatg ttggcgggtc
     2761 ggaattatat tgtcccgggt cagagtcggt ggggagatgc ggatcgctct tcataatggc
     2821 aactgcctcc cctgcggctg gtttaatccc ctgccagacc atgactccaa ggatcaccaa
     2881 tacggcgagt ccggttccgc cccagatgat gcctgcaagc agtttttgtt ttctatttct
     2941 ttgttgaatg tattgttgtc tttgtttgtt gttcatgaat tcactctgcc ggctggaaag
     3001 caggcgtcac atcgggtaac gcctgccttc gacggctgat tattgcactt tgaactcgat
     3061 atcttccttg taatccagac catccttgcg gacatacacg gtaatcttcc aattgccgct
     3121 catgctgaag ttggcattga tggaatagcg cccttcaccc tgctcggtgg cgagaccgct
     3181 catccccatg ccggtcatgt ccgtgtggtc ggcagaaaca tccacagtcg caccctcgat
     3241 gggcttgcca tctccatccg tgatcgtcag gatcaattcc atgttgccca tcatggcagg
     3301 attgggattg gtctcaatct ggatatttac cggactcgac gatcctgact cgaccggggc
     3361 ggcaggctgt gcggagctgc cgcaagccgc caatacacct gccagtaaaa ccaacatact
     3421 gataaagaac atctttcgca tttttactct ctccttgagt attttattca acgacttcga
     3481 tcatcaacat caaaccacct ggttcaacat tgtcgttggt ggtgtggtgc aggatgtgac
     3541 agtgcaacat ccattgtccg gtctctgtgg cgacgaactc aatgtcgtag cgttctcccg
     3601 gtgcgacact gaccgtgtcc ttggtcaact gtgccacctc ggggacagga tgcccatccg
     3661 tcgccacgat cctgaagggt acaccatgca gatgcatcgg atgaatgaat tgaccaatgg
     3721 caatgaagcg cagccgaacc agatcgcctt tcttgacagt gatggtttcg gtggcaggga
     3781 aggacttgcc gttgatcgtg aagtaattgg gctccatccc actcatgggc atggcggcat
     3841 acgtttgccc atccgtcata cgccattccg agatcatcag ggttttatcc acaacgggtg
     3901 gattggcatc cggttccttt gggtcaatga tgaagggcgc ataaagcccg gcgccaacct
     3961 gcacatcgcc ctcataatgc gagtgataca tgaacgtccc agccggcttg gcggtgaact
     4021 cgtaagtaaa cgtctcaccc ggctggatcg gatcctgggt aacgcccgga acgccgtcca
     4081 ttgcatttgg gacttcgact ccatgccagt ggatcgtagt tggatcgggt aattcgttct
     4141 ttacgatcac ccgaacctga tcaccttccg taacgcggat catcggtccg ggaacggttc
     4201 cgttatatgt gaatgcggtg acggtcactc catccaggat ttcccattga acagccttgg
     4261 tggttaactc aaagaccttg acgccatctt ccaggcggta ttccaagggc tgtccgccat
     4321 cagcttcggt ggcaggctgg acgttgggag cagtgatcgg ctccatcata tgagccgctt
     4381 cggggttttc catcatcatg ctgcccgggg tgggcgtccc atccgccata cctgaattgt
     4441 cattattcat gggcatgtcc ccattggagg ctgtggctgg cgcgcaagcc gataatatca
     4501 aagcgagact cgttattgta aaaaataaaa tcttcttcat tatggttcct ctgcaaagtt
     4561 ggaatagtgc ttgaatataa ggatttcccc ttgatccatc cagcgcaatt tgacgcggga
     4621 atccataggc aatattgcct atgtgaatat cagacagaaa ctgccgccct gatatccaat
     4681 gcctctttga tggaggcgac caacttttta tctatccgtt gggatgggtc atcggcaatg
     4741 gagaagcgcg tttgtccgtc acttccgtgc aggatcagtg ggacaggttc agcagactcc
     4801 ccataaacca ttagaacaac catctggcaa tcacagtccg ctgtgccatg atgcgggcag
     4861 ggacaatcct gctgggtatg tcgcgcggta tgcaggtcaa aggtttgcaa agcccgcaaa
     4921 ccaccttgca ataatttttt cttcgctgcc tgcaaagcca tgtcgcaggg cagatcaaca
     4981 atcaggaagg aagatgtggt catggaacac ctctatcatt tggatgttct ccattgtagg
     5041 tatgtttcca atcgaatagg agagccgcgt ggcgtattcg ggcttaggca atattgccta
     5101 taagaaatcc cctgtcattg taccgacagg ggatttcttg ggctgcaaac ttcagattct
     5161 cacggcatgg gcatattcat gccaccctcc gggatttcaa gttgaatttc cgcaggggca
     5221 ttccccggcg cgggaagcgg cgagaagcta atgccattgg gcgccatgcc cgttgggacg
     5281 gtggcgacaa ccgccatttt ttccaagtca atgaccgata ccgtgtcttc atagatattc
     5341 gatatgtagg cgtattggct cgatggatca atcacgactc cgtgtgcgcc ctgccctgtt
     5401 tcaatggttg caatcacctg gaaagtcgcg acatccacaa ccgacacagt ggtgcttggg
     5461 gtatcctttg tcccttgatt ggcggtcaga ataaacttgt tgttgggggt cacaaaaacc
     5521 tggatcggac ctccgcccac atccaccttg ccgaccagtt cctgcgcagc aacgtccacc
     5581 ttgccaagcg cgttttcgcc gttgagggag aagtaaacaa actccccgtc gggcgaaaag
     5641 cccacttgaa caggatgctc gccaacttct atatcggcga cctttacatc ctgcgcggtg
     5701 tcaatcacgc tcagggtggt ggatttggca ttggcaacgt aaacccacat cccgtcgggg
     5761 ctgacgcgca aaccgtgcgg atactcgccg accgggatcg tggcaatggc agacatcgct
     5821 tccacatcaa tcacagtgac cgtattatcc ccgccgttcg tggcatacgc ttttttgcca
     5881 tcgggtgtca gcacgatatg tgcgggcatc atcccagttg gcacgacacc atgaacctca
     5941 aaagtgacgg catcgatctt aatcgccagt gagtcgtgcc cgctcaccgc ccagacggtt
     6001 ttgccatccg gggcaacctg taggttgtgg gcgccctcga ctccggtaag cgtggtcagg
     6061 acttgattcg ttgccgcatt gatcacggta atgctgttgc cctcctcgtc tgccacccaa
     6121 actgttccat ccaatcctgc aacgggttga tccggttggg tctgggcggg ttgttcggtc
     6181 atttcaggtt tcgccggcgc gccacatgca ctcaagacaa ccgtggcgat gatcagcgcg
     6241 ggcataatct tccaaatcaa aaacttattc atatggttca actccttctg gttggatcga
     6301 atatcgtccg cacttataca gcacccaccc tcaagttgaa catacgcatt tttgcctaat
     6361 acgtcatgct ttatccaaat tactcacaaa ttcgatttca aactttatct tcttcaattc
     6421 gaacatattg atcgggaaca cgttcaaaaa gatgtttgca gacctcggag caaaagtaat
     6481 aagtcctccc tttgtgaaca acctgaaagg gcgggttctt tggatcgact ttcatttcac
     6541 acacaggatc gatttcaaac tcctgcccgg tcggataaaa tttccgcata ttaaaactat
     6601 tcatgatact tctagtctcc tattggctca ccgcatgaag aacaaaagcg agcgttcgat
     6661 tcgttttgag ttcccctcga tcacacatgg aaactactta acggctgaaa atcctacccg
     6721 atgaatgtaa aaaacacatg aagaattatc gaagatcaac ataggcagtt ttgcctacgc
     6781 tttgttcagc cacttggcgc aagacgccgc ccactgcaag ggctatgatg acacaaaagg
     6841 aatattttcg atgaaacgat tcttcattcc atttgtggca ttcctgctcg gctcggcgct
     6901 gatcgcctcg ttctacatcg gcatcctcac ctgggcgcag ggctgggact acgcctcatc
     6961 tcaattcatc cgtgaccgtt ggtatgtcat cccgatcatc ctcggctttg gtatccaagc
     7021 cgcgctctat tccatcctgc gattccgcct gttcattcca gttacatcca caggacatgc
     7081 cggctcaatg ataggcgcga gcggaggcac atccgccact gcgatggtcg cgtgttgcat
     7141 tcatcatgtc accgatgttt tacccattct tggtttgagt gcggcggcaa gttttctcac
     7201 ccgttatcaa cgccccttca tgcttatcgg a
//
LOCUS       DAOQJE010000052         7226 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_5710_length_7226_cov_5.418352, whole genome shotgun sequence.
ACCESSION   DAOQJE010000052 DAOQJE010000000
VERSION     DAOQJE010000052.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 7226)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7226)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7226
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5710_length_7226_cov_5.418352"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1897
                     /locus_tag="PLT08_02350"
     CDS             <1..1897
                     /locus_tag="PLT08_02350"
                     /inference="COORDINATES: protein motif:HMM:NF037660.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HNE03332.1"
                     /translation="EAGQFIRTFGGGHRHVTDYLTDEVLKRQDSATQSFLLQTSLLEK
                     LNAALCDHVLERDDSQSVLELLERKNLFLIPLDDTRQWYRHHPLWAEMLQVRLKREQP
                     EKIKMIHARAFEWFAKNDLQDEAISHALAAGEMDQAAGQIEAIAKEKVKRGESATLQG
                     WLSKLPHESILARPSLVIAQCWGLITDGRLDEVETLLGEINGRADVTPIQQGEVAAIR
                     AILATVRQDIPSIHGYAEEALRLIPLEESSIRCGVLLSQGTAAALSGDVMRAIELLTQ
                     TIQESQRGHQPIIHLIAISTLAQTYEALGDFDQAERLHRQVIAFESDSALSGLPLIGV
                     GYVGLGGVLHERLRFDEAESALQKGLEIGQRWGSPEILIGAHLSLARLRFTQGRFDEA
                     QAILETLETEFAPSMPSHESSVLEAIHARVHLAQGQRSRAEAWAEKFSAAEQTSPSLG
                     GEIQWLVLARLYLSRREFDRAEELLTQLEGNARAGARVPLLEILLLKAQLPHADARSQ
                     DALLQEALRMAEPQNQRRVFLDEAGLLPLLQAYRAKHADDLFAASLVDDFERRAVAQN
                     PSPLLSEREMDVLRLLATGLSNQEIADRLVVALSTVKSHVKNILMKLEAENRTEAVAR
                     ARELKLL"
     gene            2063..3028
                     /locus_tag="PLT08_02355"
     CDS             2063..3028
                     /locus_tag="PLT08_02355"
                     /inference="COORDINATES: protein motif:HMM:NF014601.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase N-terminal
                     domain-containing protein"
                     /protein_id="HNE03333.1"
                     /translation="MKTLIVGAGIIGTIYGWAFAEAGHEVTHLVRPGKALQFKNGFEI
                     DMYDVRKGHKRGFIGHYPIRVTETLDPSDGYELVIVPTKHYHLLDVLKQIVPQAGSAD
                     FFLLTQNWDGTEELDAILPQSRYIFGDAKAGGKFDGDKLIATIASVDVGQVSNRHDAC
                     LDKVVELCKSAQVEVNVHENILHYLWVQYATTGGLWPALVKAGSFEAVLESRQIGEQG
                     LRAARESLEVAARRGVDLSKYPETKMYMGNSPIGMWVAGIAIKAMFRFNKLVQRSSLH
                     GLSDIEEVKAFYYDLLNTGRSLNVSMPAMSAFEKDILEFEEKQRT"
     gene            3389..4678
                     /locus_tag="PLT08_02360"
     CDS             3389..4678
                     /locus_tag="PLT08_02360"
                     /inference="COORDINATES: protein motif:HMM:NF025019.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNE03334.1"
                     /translation="MNENPWKSRWSNEQIKTMLFEQFDAFWNRETGIERTQLAEVERA
                     TSIPHAVIVSGLRRAGKSTLLAQMAHKLGRDSFYYVNFEDDRFLGFQPDDANHLYHVL
                     VETFGERKIFVVDEVQNISGWEHFVRRFMDMDFKFYITGSNASLLSRELGTRLTGRYV
                     PIELFPFSFREFLQFKKETVPDLQRMTTVEKALLNNLLNTYLQTGGIPDALKYPELPL
                     LRTLYDDVLYRDIATRYRLEAVTALKELAFYLMSNPSSLVSFNKLKDQFRLGSVNTIK
                     SYIDYMENSWLVFTLKLYDFSVKRQQIAPKKVYCIDTGLSNTVGFGFSPNTGKMLENL
                     VFLALRRQTKEIYYCATPSGYEVDFYLPKKRQLIQVTRDLENPATREREVRALSDALK
                     GIKVESALILSDTNENEFEIEGIRVETRSVAEWLISQ"
     gene            complement(4768..5097)
                     /locus_tag="PLT08_02365"
     CDS             complement(4768..5097)
                     /locus_tag="PLT08_02365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE03335.1"
                     /translation="MNALKTLTVSLLAVDEAHCISEWGHDFRPDYLHIAQARAALGNP
                     LTAALTATATPQVQKDIVQLLGLGDATRIVTGFNHPNLTRDVKYASERYHFHIHSKSR
                     PSNCQIG"
     gene            complement(5164..5460)
                     /locus_tag="PLT08_02370"
     CDS             complement(5164..5460)
                     /locus_tag="PLT08_02370"
                     /inference="COORDINATES: protein motif:HMM:NF016724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HNE03336.1"
                     /translation="MDIRAKLYNHFGFSTFRAGQEEAIRSLLSEQHTLAVMPTGAGKS
                     LIFQLAALQLTGITLVISPLIALMKDQVDSFNRRNISAAYINSALPASEQNLRL"
     gene            complement(5576..5902)
                     /locus_tag="PLT08_02375"
     CDS             complement(5576..5902)
                     /locus_tag="PLT08_02375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE03337.1"
                     /translation="MDTRIAKKAASTFAEAAFFAAFGFFSTPYFGNTSLAVTKYGIPL
                     RLHRQGNIGMADGGNRRGIHGRFSLKMGVFYSDLLQNYRVRLRTHRRLEVLGKRILYS
                     KMLSEV"
     gene            complement(5902..>7226)
                     /locus_tag="PLT08_02380"
     CDS             complement(5902..>7226)
                     /locus_tag="PLT08_02380"
                     /inference="COORDINATES: protein motif:HMM:NF019368.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004559 - alpha-mannosidase activity
                     [Evidence IEA];
                     GO_process: GO:0006013 - mannose metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycoside hydrolase family 38 C-terminal
                     domain-containing protein"
                     /protein_id="HNE03338.1"
                     /translation="ETLQTIVSHLDADLLLVNPTSFTQPALVFIKGDSLQRFTLDGQP
                     VPVQQTDSGCWLDAGKLAPYSVTVLHQTSKVESQTSLADLSPETLKLENEHLIVEFNA
                     AGDIIHIFDKKANREILPPNTVANQFQAFEDRPKSWDAWDVDIFYDDKMWLAEPAFSI
                     RFIEYGKLRQTIEIKRKIQNSEYTQRISLSHNSPRLDFDTHINWQERHTLLKVAFPVN
                     VLSPQATYEIQWGNVTRPTHRNTSWDWARFETCAQKWVDLSEGDYGISLLNDCKYGHD
                     IYDNVMRITLLRSPTMPDPTADFGEHEFKYSLYPHAGGWNEETQREAYLLNDPIISYR
                     SKVESRRSANLISNFQSLISCSSPNIIIETVKQAEDGNGLIIRLYESQRKRRSITLTT
                     AFLLERVEIVNLLEETQNLLNTEGNQIHFQIKPYEIVTMRLVTYSPTG"
BASE COUNT         1739 a         1734 c         1923 g         1830 t
ORIGIN      
        1 cgaagcgggg cagttcatcc gcacctttgg cggcggacat cggcatgtga ccgattacct
       61 gaccgatgaa gttctgaagc ggcaggattc cgccacgcaa tcttttttgc ttcagaccag
      121 cctgctcgaa aaattgaacg ccgcgctgtg tgaccatgtc cttgagcggg atgactcgca
      181 aagtgtgttg gagttgctgg agcgcaaaaa tctctttctg attccgctgg atgatacgcg
      241 tcaatggtat cgccatcatc cgctgtgggc agagatgttg caggttcgct tgaagcgcga
      301 gcagcctgaa aaaataaaaa tgatccacgc ccgcgcgttc gagtggttcg cgaagaacga
      361 tcttcaagat gaagccatca gccacgcact tgccgcagga gaaatggatc aagccgcagg
      421 gcagatcgaa gcgattgcca aagagaaggt caagcgcgga gagagtgcca cgcttcaagg
      481 gtggctgtca aaacttccgc acgagtccat ccttgcccgc ccgtcgttgg tcatcgcgca
      541 atgctggggt ttgatcaccg atggcaggct cgatgaagtc gaaacgctgt tgggtgagat
      601 caacggtcgc gccgatgtga cgcccattca acagggagag gtcgccgcca ttcgcgccat
      661 cctcgccacc gtgcggcagg acattccgtc gatccacggc tatgctgaag aggcgctgcg
      721 tttgatcccg cttgaagaaa gttccatccg ctgcggcgtg ctgctcagtc agggaacggc
      781 ggcggcgctt tcgggtgatg tgatgcgcgc gatcgagttg ctcacgcaga ccattcaaga
      841 aagccagcgt ggacatcagc ccatcattca tttgatcgcg atcagcacgc ttgcccaaac
      901 ctatgaagcg cttggggatt ttgaccaagc cgaacgattg catcgccagg tgatcgcttt
      961 cgaatcagac tccgccttga gcgggttgcc attgatcggg gttggatacg tcgggttggg
     1021 cggagtcttg catgaacgcc tgcgcttcga tgaagccgag tctgctttgc aaaagggctt
     1081 ggagatcggt cagcgttggg gcagccctga gatcttgatc ggcgctcatt tgagtctggc
     1141 gcgcttgcgt tttacgcaag gcagattcga tgaagcgcaa gcgatccttg aaacattgga
     1201 gacagagttt gcgccatcca tgcccagcca cgaaagcagt gtgctcgaag ccattcatgc
     1261 gcgcgttcat ctcgcgcagg gtcagcgctc tcgtgccgaa gcatgggcag aaaagttttc
     1321 agccgcagag cagacgtcgc cctcattggg cggtgaaatt caatggttgg tattggctcg
     1381 cttgtatttg agtcgccgtg aatttgatcg cgccgaagaa ttgctcactc aactcgaagg
     1441 aaatgcccgc gcaggtgcgc gtgtgccgtt gttggaaatt ttgcttttga aggctcaact
     1501 tccccatgcg gatgcccgct cgcaggacgc gcttttgcaa gaagccctgc gcatggcaga
     1561 gccgcaaaat cagcgccgcg tctttttgga tgaagcggga ctcctgccgc tgctgcaagc
     1621 gtatcgtgcc aagcacgccg atgacctttt tgccgccagc ctcgtggacg atttcgagcg
     1681 ccgtgctgtc gcgcagaatc catctccctt gttgagcgag cgcgaaatgg acgtgttgcg
     1741 cctgctcgcg acgggtctat ccaaccagga gatcgcagat cgtctggtgg tggcgctgtc
     1801 cacggtcaag agccatgtca aaaatatcct gatgaaactc gaagccgaaa accgtaccga
     1861 agcggtggcg cgtgccagag agttgaaatt gctctaaatg aacttgcttc catcgcgtcc
     1921 tcggcggcgg ggatggcgcc ttgagcgtag acttcccgtt ccccccaccc aatcctcctt
     1981 tcgagggagg cgttcctccc tcctcgcaga taagatgtgt ccatcgttac agatggacat
     2041 tttcttttca aggagacaaa acatgaaaac tttgatcgta ggcgcaggca ttatcggaac
     2101 catttacggc tgggcatttg ccgaggcggg gcatgaagtg acgcatctcg tccgccctgg
     2161 gaaagccctc caattcaaga acggattcga gatcgacatg tatgacgtcc gcaagggaca
     2221 caaacgcggt tttatcgggc actatcccat ccgcgtgacc gagacgcttg atccaagcga
     2281 tggctatgaa ttggtcattg tcccgaccaa gcattatcac ctgctcgatg tgctcaaaca
     2341 gatcgtccct caggctggca gcgccgactt cttcctgctc acccaaaact gggatggaac
     2401 cgaagaactc gatgccatcc tgcctcagtc tcgttacatc tttggcgatg ccaaggcagg
     2461 cggcaaattt gacggggaca aactgatcgc caccatcgcc agcgtggatg tgggtcaggt
     2521 gagcaacaga cacgacgcct gtctggataa agtggtcgag ttgtgcaagt ccgcgcaggt
     2581 ggaagtcaat gtccacgaga atattctgca ttatttgtgg gttcagtacg caaccacagg
     2641 cgggctgtgg ccggcattgg tcaaggcggg cagtttcgaa gccgtgctgg agagtcgtca
     2701 gatcggagaa cagggattac gcgccgcgcg cgaatcgctc gaagtcgctg cccgtcgcgg
     2761 cgtggacttg tccaaatatc ccgagaccaa aatgtacatg ggcaattccc ccatcggcat
     2821 gtgggtcgct ggtatcgcca tcaaagccat gttccgcttc aacaaacttg tgcagcgcag
     2881 ttcattgcac ggattgagcg acatagaaga agtgaaggcg ttttattacg acctgctcaa
     2941 cacagggcgc agtttgaacg tctccatgcc tgcgatgagt gcgtttgaga aggatattct
     3001 ggagtttgag gagaagcaga ggacgtaaaa acggtctatt ttgtatgatc ttggaaggtg
     3061 cagagtggta cacttaattt gattttcaac acactgcttt cctaacggga atatcatatg
     3121 aacccttctt ctgcctctgc catcagccat ccgaatatcg ccttggcgat gtaatctcaa
     3181 gggaacacaa tatttggtgt ctgcaaggct tgttttatct atatatgcta tacccactcc
     3241 tataatcatc tatcaaaaag attccccgac aaatgatgag ggtggtaaac ccgcccggta
     3301 gcctgaacca aaatccgact agagatagat aaaagcacgc caaatagttt tcattgacag
     3361 tgaaaactat ttggcgtatc atttcattat gaatgaaaat ccttggaaat cccgctggtc
     3421 aaatgaacag attaaaacca tgctctttga acagttcgat gccttttgga acagggaaac
     3481 tggtatcgag cgcacacaac tggcagaggt tgagcgtgcc acttcaatcc cccatgccgt
     3541 aattgtctca ggtctccgtc gtgcgggaaa atccactttg ttagcacaaa tggctcacaa
     3601 gctgggacgc gattcttttt actatgtgaa cttcgaggat gaccgcttcc tgggtttcca
     3661 gcctgatgat gccaaccacc tttaccatgt tctggttgaa acctttggcg aacgcaaaat
     3721 atttgtagtg gatgaagttc agaacatatc tggttgggag cattttgtgc gccgttttat
     3781 ggatatggat ttcaagttct acatcacagg ttccaatgcc tcactactaa gccgggaact
     3841 tggtacccgt ctcactgggc ggtatgtgcc gattgagttg tttcccttct ccttcagaga
     3901 atttcttcag ttcaagaaag aaactgttcc tgatttacag cgcatgacca ctgttgaaaa
     3961 agccctcctg aacaatctcc tgaatactta cttgcaaact ggcggtatcc cagatgcctt
     4021 gaaatatcca gagctgccct tattacgaac tttatatgat gacgtgcttt accgtgatat
     4081 tgctactcga taccgtttgg aggcagttac ggcgcttaaa gaactcgcat tttatctgat
     4141 gagcaatcca tccagcctgg tttcttttaa taaactcaaa gatcaatttc gtcttggcag
     4201 tgtaaatacg attaaaagct atatcgacta catggaaaat agttggctgg tttttacatt
     4261 gaagctgtat gatttttcgg ttaaacgcca acagatcgcc cccaaaaaag tgtattgcat
     4321 cgataccgga ctttccaaca cggttgggtt tggtttttct cccaataccg ggaagatgtt
     4381 ggaaaatttg gtattcctag cgctgcgacg gcagaccaaa gagatttact actgcgccac
     4441 accaagtggt tacgaagtgg atttctatct tcctaaaaaa cgccagctaa ttcaagtaac
     4501 cagagatctg gaaaacccgg caacccgtga acgtgaagtc cgtgcattga gtgacgccct
     4561 gaaaggcata aaagttgaaa gcgccttgat cctgtcagat acaaacgaga atgaatttga
     4621 aattgaaggg attagagtcg agactcgttc agttgcagag tggttgatta gccagtaggg
     4681 cttctttgat gaatctgact tgcaaaatcc acactcagaa caaactcggt tgattcggct
     4741 tgggcagaat actggcggct ttttccttca tccaatctgg caattagatg ggcggctctt
     4801 tgaatggatg tggaaatggt agcgctccga agcgtatttc acgtcgcgag tcaggttcgg
     4861 gtggttaaat ccagttacga tacgggttgc gtcgcccaat ccaagaagtt gaacaatgtc
     4921 cttttggact tgcggtgtag cggtggcagt cagcgcggcg gtaagcggat tgcccaatgc
     4981 ggcgcgagct tgcgcgatgt gtaaatagtc agggcgaaaa tcgtgacccc attccgagat
     5041 gcagtgggct tcatccacgg caagcaggct gaccgttagc gttttgagcg cgttcaaaaa
     5101 ttgcacgttg cgcaggcgtt cgggcgcgac gtatacaagg cgatatttat tttgtgaaag
     5161 gttttacagg cgaagatttt gctcggaggc gggcaatgcg ctgttaatgt atgcggcgga
     5221 gatgttgcgt cggttgaagc tgtccacctg gtctttcatc aacgcaatga gcggtgagat
     5281 gacgagtgta atgccagtaa gttgtagcgc ggcgagctga aagataagcg acttgcccgc
     5341 gcccgtaggc atgaccgcaa gtgtgtgctg ttcgctgagg agcgaacgaa tcgcttcctc
     5401 ctgccctgcg cgaaaagtgg aaaatccaaa gtgattgtag agtttagccc tgatgtccat
     5461 aaacactcca tgtccctgcc tttggttatt ggggtcaatg atacacgatt acgaaaaata
     5521 catgaaaaac tccttcggat aaatttcccc ttgctcctga tataaaagta taattctata
     5581 cttctgacag catctttgaa tataaaattc gtttccccaa aacttcaagc ctgcgatgtg
     5641 ttctcaagcg aaccctgtaa ttttgcagta aatcactata aaagacaccc attttcaacg
     5701 agaatctccc atgaatccct cttctgttac cgccatcagc catcccaata ttgccttggc
     5761 gatgtaatct caagggaata ccatatttgg ttacagccag gctggtgttg ccaaaatatg
     5821 gtgtgctgaa aaatccaaac gctgcaaaga aagccgcctc tgcaaacgta gaggcggctt
     5881 tctttgcaat tctggtatcc attaccccgt gggggaatac gtgaccagcc gcatggtgac
     5941 gatttcataa ggcttgattt ggaagtgaat ctggtttcct tctgtattca gcagattttg
     6001 tgtctcttcc aacagattga ctatttccac ccgttccagg aggaaggcgg tggtgagagt
     6061 gatacttctg cgttttctct ggctttcgta caggcggatg atgagtccgt tgccgtcttc
     6121 ggcttgcttc acggtttcga tgatgatatt gggtgaagag caagagatta gagattggaa
     6181 gttagatatt aagtttgcag accttcgact ttcaaccttt gacctataac tgataatcgg
     6241 gtcattgagg agatacgctt ctctctgtgt ctcttcattc catccgcctg catgtggata
     6301 caaactgtac ttgaactcat gctcgccaaa atccgcggtg ggatcgggca tggttgggga
     6361 acgaagcagg gtgatacgca taacgttatc gtagatgtca tgtccgtact tgcagtcgtt
     6421 gagcaggctg atgccgtaat cgccttcaga gaggtctacc catttttggg cacaggtctc
     6481 gaagcgagcc cagtcccaac tggtgttgcg gtgcgtagga cgggtcacat tgccccattg
     6541 gatttcgtag gtggcttgtg gtgataacac attcacaggg aaggcaacct tgagcagggt
     6601 atggcgttcc tgccagttga tatgtgtgtc gaaatccagc cgcggggagt tgtggctcag
     6661 tgaaatgcgc tgggtgtatt cgctgttttg gatcttgcgc ttgatctcaa tggtctggcg
     6721 taatttgccg tattcaataa atcgaatcga aaaagcaggc tctgccagcc acatcttgtc
     6781 atcgtagaag atgtccacat cccatgcgtc ccatgatttc ggacgatctt caaaggcttg
     6841 gaattggttg gcaacagtgt tgggcggaag aatttcgcgg ttcgcttttt tgtcgaagat
     6901 atggataatg tcgccagcgg cattgaattc gactattaga tgttcattct ctagtttgag
     6961 agtttcgggt gataaatcgg caagtgatgt ttgactttcg acctttgagg tttgatgaag
     7021 tacagtcaca ctatacggcg cgagtttgcc cgcatccagc caacagccag aatcagtttg
     7081 ctggactgga acaggctgcc cgtcaagagt gaagcgttgc aacgagtcgc ctttgatgaa
     7141 gaccagcgct ggttgggtga acgaagttgg gttgacgaga agcaggtctg catccaaatg
     7201 agagacaatg gtttgcaggg tttcat
//
LOCUS       DAOQJE010000053         7082 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_5878_length_7082_cov_5.417105, whole genome shotgun sequence.
ACCESSION   DAOQJE010000053 DAOQJE010000000
VERSION     DAOQJE010000053.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 7082)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7082)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7082
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5878_length_7082_cov_5.417105"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..331
                     /locus_tag="PLT08_02385"
     CDS             <1..331
                     /locus_tag="PLT08_02385"
                     /inference="COORDINATES: protein motif:HMM:TIGR01764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="excisionase family DNA-binding protein"
                     /protein_id="HNE03339.1"
                     /translation="AQLQAADAMREAANNPNGGAGLTAGIGAGMGIGNLMGQALQGGM
                     QTGGAPAAGGGSKMPDIMTPAEAAQFMKVTEDDIMAAIQDGSLKAKKVGKAFRISKDN
                     LESFMNS"
     gene            complement(385..1938)
                     /locus_tag="PLT08_02390"
     CDS             complement(385..1938)
                     /locus_tag="PLT08_02390"
                     /inference="COORDINATES: protein motif:HMM:TIGR00196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052855 - ADP-dependent NAD(P)H-hydrate
                     dehydratase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HNE03340.1"
                     /translation="MKLVTVSQMRAIEKEADAKGISYAQMMQNAGCGLAEIVHVIGQE
                     HGWTNVVGIVGSGNNGGDALVALTWLAKAGWKTQAYVVKRQDADEELVKKYLDAGGQI
                     LQHADDVSLNTLRDFLEDVDILIDGLLGTGIKLPLRKDASHVLQEISHLLLKRKNSPF
                     IVAVDCPSGIDCDTGECADETLHADLTITMAAVKQGLLKLPAYEYIGKLELVDIGLPK
                     DLDSWDALQIDVADADIVSAILPKRTPASHKGTFGTAFVVAGSTSYTGAALLAGSAAY
                     RIGAGLVTMAIPAPLHTALVGHLPEATWVPLPHEHGFISEEASYYVLSNLERATSILI
                     GPGFGNKHTTKLFMEHLLDFLELPSVIDADGLRHLAGIHDWYKKIKVQAILTPHPGEM
                     SALTGLAISEIQSNRQEIAVKYAKAWGHVVVLKGAFTVIASPSGRITIIPVATAALAR
                     AGTGDVLAGLIAGLRAQGVEAYEAAIAGTYIHAQAGLYAADRLGTTASVLASDVLEAI
                     PRVISELEQ"
     gene            2006..3094
                     /locus_tag="PLT08_02395"
     CDS             2006..3094
                     /locus_tag="PLT08_02395"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HNE03341.1"
                     /translation="MNPKQETLLKRATLLNENPRLKKNNFDLLRLALAGIVCFVHAYQ
                     LSGYESLSFIPKILSSEAAVKGFFVASGFLIFMSYEQSSSLLSYFSRRIRRIYPAYLT
                     IIIIMAFGFVLISTKNAAEYFFSSEWIKYLAANLVFLNFLQPSLPGVFESNIFTAANG
                     ALWILKIEVMFYFSVPIIVYLFKYIPKNWVLFGGYIFSIIYSTAFNIIASQTGKELFQ
                     EIGRQLPGQLCYFLAGAFFYYNLSIFEKYITYFIVSSSIALIINQFYTIHWLEPFALA
                     TLIMFFSLFLYIGEIGKYGDFSYGIYILHSPILQIFIKYRWFSENPWLYLLIGLLTTL
                     TLAILLWHGVEKRFLLRNSHYLSNPTRS"
     gene            3110..4150
                     /locus_tag="PLT08_02400"
     CDS             3110..4150
                     /locus_tag="PLT08_02400"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HNE03342.1"
                     /translation="MQKLKSIQALRGIAVITVVLLHLKLVERKYSPTNFLLPDWLDFG
                     IFGVDLFFVISGFVMVTVTQEKFQSSKSSLLFLYHRVSRIYPLYWVYTTIALIVFKIQ
                     PSWVNSSQGNQANILSSYLLLPDNLLPLVQVGWTLIHEMYFYVVFFAIMLFLPGKYLP
                     IALLLWGGTIAMFGPSTINPFYNLILHPLTIEFIAGCFIAMYFNYVQPFRLNGLLLIF
                     FSFISILVAILSFVYFRSQTGYAPDNWDRVLYYGIPAALIVFFIVNAEKNSLSLPQFL
                     IQVGNASYSIYLSHLFTINAIGKIWSIFNSSSIIDNFFIVPATIGMVLFIGFASYWYV
                     EIPLLNLSRKLA"
     gene            complement(4152..4940)
                     /locus_tag="PLT08_02405"
     CDS             complement(4152..4940)
                     /locus_tag="PLT08_02405"
                     /inference="COORDINATES: protein motif:HMM:NF012797.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HNE03343.1"
                     /translation="MEILTSTSNPFIKQIRALRQKKTRVETGTFLVEGITHVGEVIEA
                     GWEVDAIIYAPDLLTSHFAKDLLARAARLQLRLQPVSVSVIESLADKENPQGILAVVK
                     QKPTKFSDLKSVNRAVALVSPQDPGNLGTILRTMDAVGADTLFILDGGVELFHPTVVR
                     ASMGTLFWKPVVQASFSEFLEWALTNHFQLIGTSAKADVDYHSLVPQVPWVLVLGTEQ
                     KGLTPEQTTACDVTISLPMRGRVSSLNLSVAAGVLLYQFMGDRD"
     gene            5058..5726
                     /locus_tag="PLT08_02410"
     CDS             5058..5726
                     /locus_tag="PLT08_02410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HNE03344.1"
                     /translation="MFKSLFGKKSSETSKTPSTGSQSEGMEKFLKSMNLSYEDWRNGQ
                     SYDLNVLKNLSPAETQEAERILISRKDEDFRDVEALATLGTPATIQALRDCLQSQNLS
                     NRFHAAKYLKETGEDHVEEVIFSTLHITQIGNGMVEGLRLAKEYPSDKIKHRVVWCTF
                     HGNDGIRTHCAAMALYLYGKADSDFDNKQQIIFRVGAKNKEMGNKVFPEFCELLGVNP
                     EDFA"
     gene            5796..7016
                     /locus_tag="PLT08_02415"
     CDS             5796..7016
                     /locus_tag="PLT08_02415"
                     /inference="COORDINATES: protein motif:HMM:NF019142.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="G5 domain-containing protein"
                     /protein_id="HNE03345.1"
                     /translation="MKLAPWLVLLLAFSLVSCQSLSPRLVTILDGDTVLPVTTSETVP
                     LLILADAGITPQPTDRVLVNGIPYKLDQDISLDGDWHLQLIRSISVKLITPQGEQNLQ
                     TSALTVGDFLKENGFATSLNDKVTPPLTTPITDSLTITFSPAQTMSISAGETSLTINS
                     SAQTVGEALAQAGIPLMGMDTSVPAENEPLPIDGQITVTRVTESVLVELQSIPFSTEE
                     TESVDVAFGEQNILQTGENGIAMVKTRIRYENGVEVNRVEEDKTILKEPVKQIVAKGT
                     KIVLSEVGGSAPYQYWYATQMYASWYSPCNSGTGGCSYGTASGAKAGFGIVAVDYSYY
                     SYLAGMRVYIPGYGVATIGDTGGGPVIESTFGVPRYQWIDLGYDDNNIGGLSGWVTVY
                     FLEPAPAEIPYFFK"
BASE COUNT         2040 a         1534 c         1410 g         2098 t
ORIGIN      
        1 cgctcaactg caagctgcag acgccatgcg cgaagccgca aacaacccga atggtggagc
       61 tggtctcacc gctggtattg gcgccggaat gggcattggt aacctgatgg gacaagccct
      121 tcagggtggt atgcaaactg gtggcgctcc cgccgctggt ggtggatcaa aaatgcctga
      181 tatcatgact cctgctgaag cagctcagtt catgaaggtc accgaagatg acattatggc
      241 agccattcaa gacggaagcc tgaaagccaa gaaagtaggt aaggcgttcc gcattagcaa
      301 ggataacctt gaatctttta tgaatagcta gtttataagg aaaaaaaaag aggctttcat
      361 tacgaaagcc tctttttttt tattctactg ttccaactca gagatgactc ttggaattgc
      421 ctctaacaca tcactggcaa gaaccgaagc tgttgttcca agtctatcag cagcatatag
      481 cccagcttgt gcatgaatat atgtccccgc aatagccgct tcgtatgctt ctacaccttg
      541 tgcacgaaga ccggctatta aacctgccaa cacatccccc gtccctgctc gggcaagggc
      601 tgctgtagca actgggatta tggttatacg tccacttggg gaagcaataa ccgtaaatgc
      661 gcctttcaat acaacaacat gtccccatgc ctttgcatac tttacagcga tctcttgccg
      721 attagattgt atctctgaaa ttgctaaccc agttagcgca gacatctcgc caggatgtgg
      781 agttaggata gcctgcactt ttattttttt ataccaatca tgaattccag caaggtgccg
      841 cagtccgtct gcatcaataa cagatggcaa ttccagaaaa tccaataaat gttccataaa
      901 caattttgtc gtgtgcttgt tcccaaaacc tggtccgatc aaaattgagg ttgcacgctc
      961 caaattcgac aaaacataat agctggcttc ctctgaaata aatccatgtt catgtggcaa
     1021 tggaacccaa gtcgcttcgg ggagatgacc aacaagcgca gtatgaagcg gtgcgggaat
     1081 tgccattgtg accaatcctg caccaatacg ataggcagca ctgccagcca gcaaagccgc
     1141 gcctgtatag gatgtagagc cagccaccac gaaggcagta ccaaatgtcc ctttgtgaga
     1201 agcgggcgtt cgctttggta agatggcaga aacaatatca gcatctgcga catcaatctg
     1261 caaagcatcc caagaatcca aatctttggg taatccaata tccacaagtt ctaatttgcc
     1321 gatgtactca tatgcaggga gtttgagcaa gccttgtttt actgctgcca tcgtaattgt
     1381 aaggtctgca tgcaaagttt catcggcaca ctcaccagta tcacaatcaa tgccagatgg
     1441 acaatcaacc gcgacgataa aaggtgagtt ttttctttta agtaagagat gggatatttc
     1501 ttgcaaaacg tgagatgcat ctttcctcaa cggtagtttg atgcctgtgc caagcaaccc
     1561 atcgataaga atatctacat cttctagaaa atctctcaaa gtattcaagc taacgtcatc
     1621 ggcatgttgt aaaatttgac cgccagcatc aaggtatttt ttaactaatt cttcatctgc
     1681 atcttgacgt ttcaccacgt aggcttgagt tttccatcct gctttggcaa gccaagttaa
     1741 agcaacgagt gcatcgcccc cattattccc tgaaccaaca atccctacaa cgttagtcca
     1801 accatgctct tgtccaatca catgaacaat ctcagccaaa ccacacccag cattttgcat
     1861 catctgagca taagagatgc cctttgcatc agcttctttt tcaattgctc gcatttgaga
     1921 aacagtgaca agtttcatat caattcatag ttattacagg tttataattg ttttctgcta
     1981 ctaaattgta cattgctata caaacatgaa cccaaaacaa gaaacactat tgaaaagggc
     2041 tacattgcta aacgaaaacc ctcgccttaa aaaaaacaac ttcgatttac ttcgcctagc
     2101 tcttgctggc atcgtatgct tcgttcatgc gtatcaatta tctggctatg aatcgctaag
     2161 ttttattcct aaaatattat cctctgaagc agcagttaaa ggtttttttg ttgcgagcgg
     2221 ctttctaatc tttatgagct atgagcaatc ttcttcttta ctttcatatt tttcaagaag
     2281 aattaggcgc atatatcctg catacctaac cattatcatc ataatggcat tcggtttcgt
     2341 gttaataagc accaaaaatg cggcagaata tttcttttca tctgaatgga taaaatatct
     2401 agcagctaat cttgtctttc taaattttct tcagccatca cttcctggtg tttttgagtc
     2461 taatatattt acagctgcca atggtgcact atggatacta aaaattgagg ttatgttcta
     2521 tttttcagtt ccaatcattg tttatctttt caaatacatt cctaaaaact gggtattatt
     2581 tggtggttac atattttcta ttatttattc aacagccttc aatataattg catctcaaac
     2641 tgggaaagaa ctatttcaag aaataggccg tcagttacca gggcagctat gttattttct
     2701 agcaggagct ttcttttatt ataatctttc tatttttgaa aaatacatca cttatttcat
     2761 agtctcatcc tcaatagcgc taatcataaa tcagttttac acaattcatt ggctagaacc
     2821 atttgcttta gctactctca ttatgttctt tagtttattt ttgtatatag gcgagattgg
     2881 aaaatacggg gatttctcat atggcattta tatattgcac tctccaatac ttcaaatatt
     2941 tattaagtac agatggttca gtgaaaatcc ttggctttat ctcttaatag gtttattgac
     3001 aactttaaca ttagccattt tgttatggca cggggtagaa aaacggtttc ttctacggaa
     3061 tagtcattat ctttcaaatc caacaagaag ttaggaattt acccataaaa tgcaaaagtt
     3121 aaaaagtatt caagcactgc ggggtatagc tgttattacg gttgtattac ttcacttaaa
     3181 actggttgag agaaaatata gccccacaaa ttttttatta cctgactggc tagattttgg
     3241 aatctttggt gtcgaccttt tctttgttat cagcggattt gtcatggtca ctgttaccca
     3301 agaaaaattc cagtcctcaa aaagctcttt actattctta tatcatcgtg tatcaagaat
     3361 ttatcctctt tattgggtat acaccacaat cgcattaatt gtttttaaaa ttcagccatc
     3421 atgggtaaac agttcacaag gaaatcaggc aaacatctta tcgtcctatc ttcttttacc
     3481 agataatctt cttccattag ttcaagtcgg gtggacttta attcatgaaa tgtatttcta
     3541 tgtagtattt tttgcgatca tgcttttttt accgggaaag tatctgccaa tcgccttatt
     3601 actatgggga gggacaattg cgatgtttgg tccttcaaca atcaatccct tctacaacct
     3661 aatactccat ccacttacta tagaatttat tgcagggtgt ttcattgcta tgtactttaa
     3721 ttacgttcag ccattcagat tgaatggctt gcttcttatt ttcttttctt ttataagcat
     3781 attggtcgca atacttagtt ttgtttattt tcgaagccaa actgggtacg ctccagataa
     3841 ttgggaccgg gttctttatt atggaatacc agccgcttta attgtttttt ttatagtcaa
     3901 tgctgaaaaa aacagcctct cacttcctca attcttaatt caagttggaa atgcctccta
     3961 ctcaatatat ctttctcatt tatttactat caatgccata gggaaaatat ggagtatatt
     4021 taattcatca tcaattattg ataatttttt tatagtacca gccacaattg gaatggttct
     4081 gtttatcggt ttcgccagct attggtatgt tgagatacct cttcttaact taagccgtaa
     4141 gcttgcatag actaatcacg atctcccata aactgataca acaagacacc tgctgccaca
     4201 gacaagttca gcgagctgac tcgtccgcgc atagggagag aaatagtcac atcacaggct
     4261 gtcgtttgtt ctggggtaag ccccttctgc tcagtaccta acaccaacac ccaagggacc
     4321 tgagggacga gagagtgata atctacatcc gctttggctg aggtcccaat aagttgaaag
     4381 tgatttgtaa gtgcccattc aagaaattcg ctaaaagaag cctgtacaac cggcttccaa
     4441 aacaacgttc ccatactagc acgaaccacg gtgggatgaa acaattccac gccgccatct
     4501 agaataaaga gtgtatctgc accaactgca tccatcgtgc ggaggatggt gccaagattg
     4561 ccagggtctt gcggcgaaac caaagctact gcgcggttta cagactttag gtctgaaaac
     4621 ttcgttggct tttgcttaac tacagcaagg attccttgtg ggttttcttt atcagcgaga
     4681 gattcgatga cagaaacaga gacaggctga agtcgaagtt ggaggcgggc agcgcgggcg
     4741 agcaagtctt ttgcaaagtg gctggttaac agatcaggcg cataaatgat ggcgtccact
     4801 tcccagcctg cttcgatcac ttctccaaca tgtgtgatgc cttctacaag aaaggttccg
     4861 gtttctacac gtgttttttt ctgtcgcaaa gcgcgaatct gttttatgaa agggttcgag
     4921 gtgctggtaa gaatttccat atctgaattt tatccgatat gaagtgattt ggtgggaggc
     4981 agacttttgg gggatgaaat ttaaaataaa gatacatata atttcagcat attcaattca
     5041 aacgaggaaa taattttatg ttcaaaagtt tatttggaaa gaaatcgtca gaaacaagca
     5101 aaacgccatc tacaggctcg caaagtgaag gtatggaaaa atttctcaag agcatgaatc
     5161 ttagttatga agattggcgg aatgggcaaa gttatgattt aaatgtattg aaaaacttaa
     5221 gcccagcaga gacacaagaa gcagaacgaa ttctaatttc tcgaaaagat gaagattttc
     5281 gagatgtaga agcattagct acacttggca cgccagccac cattcaagcg ctacgagatt
     5341 gcctgcaaag ccaaaatctt tcaaatcgtt ttcatgcggc aaaatacctg aaagaaactg
     5401 gcgaagacca tgttgaagaa gtcatcttta gcacgttgca tattactcaa atcggtaatg
     5461 gcatggtgga aggattaaga ttagcaaaag aataccctag tgacaaaatc aaacaccgcg
     5521 tggtctggtg cacatttcat ggcaacgacg gtattcgcac tcactgtgca gcaatggctc
     5581 tttacttata cggcaaagcc gactctgatt ttgacaacaa acaacaaatc atctttagag
     5641 ttggagccaa gaacaaagag atgggcaata aggttttccc agaattttgt gagcttctcg
     5701 gtgtaaaccc tgaagatttt gcataaatct cgaaaacgac ccgatcaaat tttcgggtcg
     5761 tttttttatg cctcaatcca tttataattg gcagtatgaa acttgctcca tggctggtct
     5821 tgctactggc tttttcgctt gtcagttgcc agtcgctctc cccacgttta gtcacaattt
     5881 tagatggcga tactgtgcta cccgttacga cgtcagaaac ggttccgctt ttgattcttg
     5941 cagacgccgg cattactcct cagccaacag atcgagtcct tgtgaatggc attccatata
     6001 agctcgatca agatatttct cttgacggtg attggcactt acaacttatc cggtctattt
     6061 cggtaaaact gattacgcct caaggggaac agaatcttca gacctccgca ttgactgtag
     6121 gtgacttttt gaaagaaaat ggattcgcca ccagcctcaa tgacaaagtc accccgcctc
     6181 ttactacgcc gataactgat tcattaacca tcactttttc tccagctcaa acaatgagta
     6241 tttcagcagg agaaacatct ctcactataa attcttctgc tcaaactgtt ggtgaagcac
     6301 tggcacaggc gggaattccg ctcatgggca tggataccag cgtcccagcg gagaatgagc
     6361 cgctcccaat agatgggcaa attaccgtta cccgggtgac agagtcagtt ctagtagaac
     6421 tccagtcgat tcctttttcc accgaagaaa ccgaatccgt tgatgtggct tttggcgaac
     6481 aaaatatttt acaaacaggg gaaaacggta ttgccatggt gaaaactcgc atccgatacg
     6541 agaatggtgt ggaagtgaac cgagttgaag aagataaaac tattttgaaa gaacccgtga
     6601 aacaaattgt agccaaaggg acaaagattg tactttcaga agttggcggt agcgcgccat
     6661 atcaatattg gtacgcaacc caaatgtatg cctcatggta ttcgccatgc aactcaggca
     6721 caggtggatg ttcgtacggc actgcaagcg gagccaaagc cgggttcggc attgtagcag
     6781 tcgattattc atattactca tatttagccg ggatgcgcgt ttacattcct ggttatgggg
     6841 tcgcaactat tggcgataca ggcggaggac cggttattga atcgaccttt ggggttccgc
     6901 gatatcaatg gatcgatctc ggctatgacg ataataacat tggcggtctc tctggctggg
     6961 tgactgtata ctttttagag cctgcgcccg cagaaatccc ttattttttc aaataacatg
     7021 acaaaacctc aacgtatcac tattgctgta ctatccgtta ttgcattttt actggcagcg
     7081 gt
//
LOCUS       DAOQJE010000054         7017 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_5960_length_7017_cov_4.965384, whole genome shotgun sequence.
ACCESSION   DAOQJE010000054 DAOQJE010000000
VERSION     DAOQJE010000054.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 7017)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7017)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7017
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5960_length_7017_cov_4.965384"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..859)
                     /gene="ricT"
                     /locus_tag="PLT08_02420"
     CDS             complement(<1..859)
                     /gene="ricT"
                     /locus_tag="PLT08_02420"
                     /inference="COORDINATES: protein motif:HMM:NF041131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="regulatory iron-sulfur-containing complex
                     subunit RicT"
                     /protein_id="HNE03346.1"
                     /translation="MQTDVKNIIGVRFTPIGKIYHFDSSSLPDLKQGEHVIVDTTRGR
                     HLGEVVQILQEAPPKPEGGWKSVERRATPRDLLLQQSWRNKQTEAMINCRARASELGL
                     LETIKVVAAEYNYDGSRLAFLFSTEQEEKVDLKSLRKDMSDIYPNTHIEMRQIGPRDV
                     AKLIGGMGACGIETRCCSKFLTDFSPISIKMAKEQGISLTPDEITGMCGRLRCCLIYE
                     YEQYVEARKTLPKRNKRVVTPKGEGKVIDVMPMSDKVMVLIEIPNERPQHHTFLREEI
                     EPWDELEALR"
     gene            complement(958..2061)
                     /locus_tag="PLT08_02425"
     CDS             complement(958..2061)
                     /locus_tag="PLT08_02425"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: protein motif:HMM:NF002872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006002 - fructose 6-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent 6-phosphofructokinase"
                     /protein_id="HNE03347.1"
                     /translation="MSATIKKIAISTGGGDAPGLNAVIRAAALSAIKRGWEVYGVLDG
                     FHGILSPEEYPDGGFMPLTRQRVRGITHLGGTILGTSNRNNPMKYPIQRPDGTIEEVD
                     RTDEIVRGFRLHHIDALITIGGDGSLAIANVLAQKGLRLISVPKTIDNDLDKTDVTFG
                     FDTAVGFATEALDRLHSTAASHGRVMVVEVMGRYAGWIALHAGVAGSADVILIPEHPY
                     DIHKVVEKIREREESGNKFTIVVVAEGASPKGGGVSVVEHEAGRAERLGGVGEKVAAE
                     IHAISGKETRVVVLGHLLRGGSPTSFDRLTALRFGAAAVRGLEEGQSGVMVALDPPHV
                     KYVPIAEAIGQMKMVPMESDIVLTARDLGISFG"
     gene            2276..3487
                     /locus_tag="PLT08_02430"
     CDS             2276..3487
                     /locus_tag="PLT08_02430"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNE03348.1"
                     /translation="MNKVVSIDKTALLRSAFNGLSENELEEMAALTEVRIYPPEHVLC
                     REGEYEETFYIIAEGTALISKYIGDQEGDRVLRTVGKGDLVGEMALIQNAPRSATVRA
                     TSELIALEMDKDHFETMLGRSPHMALDIIRITLNRLRENDQMMISELQKSNKVLQQLD
                     RNKTEFIDVVAHELRTPLTVLKGYSNLLSASPEVKNHPTLRDVSEGIQSGADRIHLIV
                     NTILDVSRIDNNELTLRRSPILLKQVVLEVLSGLTKAASERTIELFHLPGADTPLIHG
                     DPALVHKALYHLVINAIKYTPNGGKVSIATRPFKLDHGEEGVLISVQDTGIGLDPEHH
                     ELVFEKFYQAGHAAIHSSGTTSFKGGGPGLGLAIVRGVARIHGGKAWVESTGEDEVNF
                     PGSTFYLWLPV"
     gene            complement(3539..4546)
                     /locus_tag="PLT08_02435"
     CDS             complement(3539..4546)
                     /locus_tag="PLT08_02435"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNE03349.1"
                     /translation="MSTMKNIQVKHAPAIAGLSFRGFAGESDYPKMLAIIEGSKQVDQ
                     VERTDSLEDVRRNYSHLSNCDPYTDMLFAEINGEVIGYQRVWWDQLNDGVTRTYSGIG
                     FLLPAWRRKGIGTAMFQHAEERLRQIAAAHTGPETKVFSMWANGTAQGLIALLEKQGY
                     QSTRYFFEMARDIHEPLPEVSLPSGLEVRPVTEVDYRAVYEAHTEAFRDHWGFNEQTF
                     EEFMHWAEEEDFNPGLWMVAWDGDQIAGQVLNFVSNAENREYHRKRGYTEGISVRRPW
                     RKRGLAKALLVKSIAMFKDQGMSETALGVDAENPSGALGLYQSLGYKEIHRNVIYRKP
                     L"
     gene            complement(4662..5300)
                     /locus_tag="PLT08_02440"
     CDS             complement(4662..5300)
                     /locus_tag="PLT08_02440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE03350.1"
                     /translation="MNAIHALYDEGLQDLLTGMLSLSTFADAKEWSDRANEEIAHLQQ
                     SLHEIDEDVFRHTQALEKIQYDRSKKMLGGLLGASKEEKELTQQIEDLKSSRTGLSKA
                     IQQVQEMMDFTPRSLEEKHALLTELRMRKRRLQEEKREITQVVRGPRLKQPHESLSEP
                     LFNGPSRERRERRKVRYDREARLKEGETSLAALTRQIEQAERDLQWVEKFEE"
     gene            complement(5410..>5756)
                     /locus_tag="PLT08_02445"
     CDS             complement(5410..>5756)
                     /locus_tag="PLT08_02445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE03351.1"
                     /translation="VDGQGNYVFFLGIITTVGSPSSSNASLAAPGDLRYEYDCSGGIT
                     RISLFWKDKADNEQGYRVYRDGEKVADLPAGTTSYDDIAPSPGSYAYSVAAYNTGGES
                     PTKVQAETSACQ"
     assembly_gap    5757..5856
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<5857..6878)
                     /locus_tag="PLT08_02450"
     CDS             complement(<5857..6878)
                     /locus_tag="PLT08_02450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE03352.1"
                     /translation="MQPQVFFVPKEHTLEAILQFRKELATYSSESHEYIFDFQSGSGY
                     ATPFGMLGIGFAIRQFVATRPGSKVKAKNIDKSNYAAHMGYYQCCGFEVGNPPGKAKG
                     SHTYLPVTILNVEELREQARMAGSEVGDVIEERSQQLARILTQEELGATNDVIAYSLR
                     EMIRNVAEHSQSQELAYCAQYHPLKKQAELAILDTGVGIRATLSQNPHLSIHDDREAL
                     NLAVMPGISSKMYKGIKKREYDVWQNSGYGLYVVSRLCGHEGKFLICSGDKAMSLKPD
                     AREYHEAHFPGTAVRLNLSSKNISNMSKTLKAISLEGQEQERLMKAVENDHAPVASRM
                     LASEFS"
BASE COUNT         1522 a         1891 c         1839 g         1665 t
ORIGIN      
        1 ggcgcagggc ttcgagttca tcccacggtt cgatctcttc acgcaggaag gtgtggtgtt
       61 gcgggcgctc gttggggatt tcgatcagca ccataacctt atcgctcata ggcatcacgt
      121 caatcacttt gccttcgcct ttgggggtaa ccacgcgctt gttacgcttg ggcaacgtct
      181 tgcgggcttc tacatattgt tcatactcat aaatgagaca gcagcgtaag cgcccgcaca
      241 tgccggtgat ttcatcggga gttagtgaga tgccttgttc cttcgccatc ttgatcgaga
      301 tgggggagaa atcggttagg aacttggaac agcaacgggt ttcaatgccg caagcgccca
      361 tgccgccgat aagttttgcc acatcacgcg gaccaatctg acgcatttca atatgcgtgt
      421 ttggatagat gtcgctcata tccttgcgca gcgatttgag gtccaccttt tcttcttgtt
      481 cggtgctgaa caagaaggcg aggcgtgaac cgtcgtagtt gtattcagcg gcaaccactt
      541 tgatggtctc cagcaagccc agctctgaag cgcgggcgcg gcaattgatc atggcttcgg
      601 tctgtttgtt gcgccatgat tgttggagga gcaggtcgcg cggcgtggcg cggcgctcta
      661 cagatttcca gccgccttcc ggtttgggag gagcttcctg aagaatctgc acaacctcgc
      721 caaggtggcg tccgcgcgtg gtatcgacga tgacatgttc gccttgtttg aggtctggca
      781 gggaggatga atcgaagtga taaatcttgc cgatgggggt aaaccgtaca ccgataatgt
      841 ttttaacgtc tgtttgcatg cgcgtaatta tacctgagac gttcaggcag agggagtttt
      901 gaggaaataa agagaggtca cagaaacctc tgtgacctct gcctgtgtat ggctgcttta
      961 cccgaacgaa atgcccagat cgcgtgccgt gagcacaata tcagactcca tcggcaccat
     1021 cttcatctga ccgatcgctt cggcaatcgg cacatacttg acatgcggcg ggtcgagcgc
     1081 caccatcacg ccagattgcc cctcttccaa tccgcgcacg gcagcggcac caaagcgtaa
     1141 cgcggtgaga cggtcgaacg aggtgggcga gccgcccctc aaaaggtgac ccagcaccac
     1201 cacacgcgtc tctttgcccg aaatggcatg gatctctgcc gctacttttt cgcccacccc
     1261 gcccaggcgt tcggcacgac ccgcttcatg ctccactacg gaaacgccgc cacccttggg
     1321 gctggcgcct tctgctacca ccacgatcgt aaacttattg ccgctttctt cacgttcacg
     1381 gattttttcc accactttgt gaatatcgta ggggtgttca ggaatgagaa tgacgtcggc
     1441 agaaccagcc acgccggcat gcagggcgat ccaaccggca tagcgaccca tcacttctac
     1501 taccatcaca cgtccgtgcg aggcggcggt agagtggagt ctgtcgagtg cttcggtggc
     1561 aaagcccaca gcggtatcaa acccaaaggt gacatcggtc ttatcaaggt cgttgtcgat
     1621 ggtcttggga acggaaataa gccgcagccc tttttgagca agcacgttag cgattgccaa
     1681 cgaaccatca ccgccaatgg tgatgagggc atcgatgtga tgcaggcgga agccgcgcac
     1741 aatttcatcg gtgcggtcca cttcttcaat agtgccatcg gggcgctgga tcggatattt
     1801 catggggtta ttacgattgc tggtgccgag gatcgtgccg ccgaggtggg tgatgccgcg
     1861 cacacgttga cgggtgagag gcatgaaccc gccatctgga tattcttcgg gggaaagaat
     1921 gccgtggaaa ccgtccaaga ctccatacac ttcccagcca cgtttaatgg cagagagagc
     1981 ggcggcgcga atgaccgcat tcaaaccggg ggcgtcaccg ccgccggtgc tgatcgcaat
     2041 tttcttaata gttgctgaca tgggaaactc ctgcgataag ggcattatct attgataaac
     2101 gttcttatcc aacatgtggg attttttaaa agtcattata gccctcaagc ctgccagatg
     2161 gacatactcc cccagaagta tagacagaaa tcatgccaag gaggcgaagc aagtcataaa
     2221 aattatcatt caagagcaat atttcgcagg caaagggctg ggtatactga cacgcatgaa
     2281 caaagtcgta tctatcgaca agactgccct cttacgctcc gccttcaacg gcttatcgga
     2341 gaacgagctg gaagaaatgg cggcactgac cgaagtgcgt atctacccgc cggagcatgt
     2401 cttatgccgt gaaggggaat acgaagaaac cttctacatc atcgccgaag gaacagcgct
     2461 tatcagcaaa tacattggtg accaggaagg tgaccgtgtt ttgcgcacag ttggcaaagg
     2521 agacttagtg ggcgagatgg cattgatcca gaatgcgccg cgttctgcca ccgtgcgtgc
     2581 cacctctgaa ctcatcgccc tcgaaatgga caaagaccac ttcgaaacca tgttggggcg
     2641 cagtccgcac atggcgttgg atatcatccg catcaccctc aaccgcttgc gcgagaacga
     2701 ccagatgatg atctctgaat tgcagaaatc caacaaggtc ttgcaacagc ttgaccgcaa
     2761 caaaactgaa ttcatcgacg tggtggcgca tgaattgcgc acaccgctga ccgttttgaa
     2821 gggctattcc aatttactaa gcgcatcgcc tgaggtaaaa aatcacccca ccctgcgcga
     2881 tgtctctgag ggcatccaaa gcggagccga ccgcattcac ctgatcgtca acaccatctt
     2941 ggatgtctcg cgcatcgaca acaacgagtt gaccttacgc cgttccccga ttctgctcaa
     3001 acaagtcgtt ctagaagtcc ttagcggatt aaccaaagcc gcctccgaac ggaccattga
     3061 actgttccac cttcccggag cagatacgcc cctgattcat ggtgacccgg ctctagttca
     3121 caaagcctta tatcatctcg tcatcaatgc catcaaatac acccccaacg gcggtaaagt
     3181 gtccatcgcc acccgtccat ttaaactaga ccatggcgaa gaaggcgttt tgatcagcgt
     3241 ccaagatacc ggcattggct tggaccctga acatcatgaa ctggtcttcg aaaagttcta
     3301 tcaagccggg catgccgcca tccactcttc gggcaccacc agcttcaaag gcggcggacc
     3361 gggcttgggc ttagccattg tgcgcggcgt ggcacgcatc cacggcggca aggcttgggt
     3421 ggaaagcaca ggtgaagacg aagtgaactt ccccggcagt accttctatt tgtggttgcc
     3481 tgtatagaga cctaaaacaa aagaggacgg ataaaaaatc cgtcctcttt ttatatcctt
     3541 acagcggctt tcgatagatc acattgcgat ggatctcttt gtaccccaag ctttggtaca
     3601 agcccaacgc cccgctcggg ttttcagcat ccacgccgag cgcggtttcg ctcatgcctt
     3661 ggtctttgaa catggcaata ctttttacaa gcaacgcctt tgccagcccg cgtttgcgcc
     3721 acggtctgcg gacggagatg ccttcggtat acccacgctt gcgatggtat tcacggttct
     3781 ccgcgttgct cacaaagttc aagacctgcc cggcgatctg gtcgccatcc caagccacca
     3841 tccatagacc ggggttgaaa tcttcttcct ctgcccaatg catgaactct tcaaaggtct
     3901 gttcgttgaa gccccaatga tcgcggaagg cttcggtatg ggcttcataa acggcacgat
     3961 agtctacctc tgtcaccggg cgtacttcca agccagatgg cagggaaact tcgggcaagg
     4021 gttcatggat atcacgtgcc atctcaaaga aataacgcgt ggactgatag ccttgctttt
     4081 caaggagggc gatcaagcct tgagcggtgc catttgccca catggagaag actttggttt
     4141 cgggtccggt gtgagccgcg gcgatctggc gcaaacgctc ttcggcatgt tggaacatgg
     4201 cggtgccgat ccctttgcga cgccatgcag gcagcagaaa accgatgccg ctataggtgc
     4261 gtgtcacgcc gtcgttaagt tgatcccacc agacgcgctg atagccgatc acctcgccgt
     4321 tgatttctgc gaagagcata tcggtatagg ggtcacagtt gctgaggtgg ctgtaattgc
     4381 gccgtacatc ctcaaggctg tcggtgcgct ccacctggtc gacctgcttg ctgccttcaa
     4441 tgatcgccag catttttggg taatcacttt caccggcaaa cccacggaag gaaagccctg
     4501 caatggcagg cgcatgcttt acttggatgt tcttcatggt agacatgttt ttattatatc
     4561 cctgtaacag ggcagtcgat tcatttgcta ggatgacgac ttgttaaatc aaaagagacc
     4621 agagaagtta tttccatctc tggtctcttg ctgttcatta actattcttc gaacttttca
     4681 acccattgaa ggtcgcgttc ggcttgttcg atctgacgcg tcagcgcggc gagactggtt
     4741 tcgccttcct tcaagcgggc ttcgcggtcg tagcgcactt tgcggcgttc acgtcgttcg
     4801 cggctgggac cgttaaacag cggctccgaa agggactcgt gcggctgctt caaacgtgga
     4861 ccgcgcacca cctgtgtgat ctcacgcttt tcttcctgca gcctgcgttt gcgcattcgc
     4921 agttcggtca gtaaggcatg cttttcttcc agcgagcgcg gcgtgaagtc catcatctct
     4981 tgcacctgct gaatggcttt gctcagccca gtgcgcgaag atttgaggtc ttcgatctgc
     5041 tgtgtcagct ctttttcttc tttgctggcg cccaacagcc cgcctagcat cttcttcgag
     5101 cggtcgtatt ggatcttctc gagcgcctgg gtatggcgaa acacatcttc atcgatctca
     5161 tgaaggcttt gctgaaggtg tgcgatctcc tcgtttgccc ggtcgctcca ttccttggcg
     5221 tcggcaaagg tggaaagcga gagcatcccg gtcaggaggt cttgcagacc ttcatcgtaa
     5281 agagcatgga tggcattcat acggctaatt tcctgggttc agtttaagtt gactgcaagg
     5341 attctaaatg aatcaaccca gcagaaatca aaagttcacg tacgaatggc taacagtttt
     5401 gttggttcgc tactggcagg cgctggtctc ggcttgcacc ttggtgggcg attcgccgcc
     5461 ggtgttgtag gctgccacac tgtaggcata cgaacccggg gaaggggcaa tatcgtcgta
     5521 ggatgtggtg ccggcgggca ggtctgccac cttttctccg tcgcgataga cccggtagcc
     5581 ttgctcgtta tcggctttgt ccttccagaa caagctgatg cgcgtgatgc cgccgctgca
     5641 atcatattca tagcgcaggt ctcccggcgc ggcaagcgag gcgttgctgc tgctgggtga
     5701 gccgaccgtg gtgatgatgc cgaggaagaa gacgtaattg ccctgtccat ccaccannnn
     5761 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5821 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnttgg agaattcact tgccagcatg
     5881 cgcgaagcca ccggggcatg gtcgttctca accgctttca tcaggcgttc ctgttcctgc
     5941 ccttccaaac tgatcgcctt gagcgtcttg ctcatgttgc tgatattctt ggaggataaa
     6001 ttcaaacgta ccgccgtgcc ggggaaatgc gcctcgtgat actccctcgc gtctggcttg
     6061 agcgacatgg ctttatcgcc gctgcaaatt aagaacttgc cttcgtgccc gcacaagcgg
     6121 ctgaccacat acaagccata gcccgaattc tgccagacgt catactcgcg tttcttgatg
     6181 cctttataca tcttgctgga gatgcccggc atgaccgcca agttgagcgc ctcgcggtca
     6241 tcatgaatag acaggtgcgg attctgtgaa agcgtagcgc ggattcccac gccggtatcg
     6301 aggatcgcca actcggcttg tttcttgagc ggatgatatt gggcgcaata cgccagctct
     6361 tgcgattgac tatgctctgc cacgttgcgg atcatctctc tcagcgagta ggcgatcaca
     6421 tcgttggtgg cacccagctc ctcctgagtc aagatgcggg cgagctgctg cgagcgttcc
     6481 tcgatcacat cgcccacttc gctgcccgcc atgcgggctt gttcgcgcag ttcctccacg
     6541 ttcaagatcg tgaccggcaa ataggtatga ctgccctttg ccttgccggg cgggttgccg
     6601 acttcgaacc cgcagcattg atagtagccc atatgcgccg cgtaattgct cttatcgata
     6661 ttcttggctt tgaccttgct gccggggcgg gtcgccacga attgacggat ggcaaagccg
     6721 atccctagca tgccaaaggg tgtggcatag ccactgccag attgaaaatc aaagatgtat
     6781 tcgtgggact cactgctata ggttgccaac tctttgcgga attgcagaat ggcttcgagg
     6841 gtatgttctt tgggaacgaa aaaaacttgg ggttgcatgg ctttccactc ctatggggtt
     6901 gtatgcaagc cctattgtat tcgatttgat gatgcagaaa ccacaaagag cacagagaaa
     6961 atgagcgttg cctctgtgct ctttgcagct aaacttttat accaagccga gcttcaa
//
LOCUS       DAOQJE010000055         6956 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_6036_length_6956_cov_6.037241, whole genome shotgun sequence.
ACCESSION   DAOQJE010000055 DAOQJE010000000
VERSION     DAOQJE010000055.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 6956)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6956)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6956
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6036_length_6956_cov_6.037241"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(144..704)
                     /locus_tag="PLT08_02455"
     CDS             complement(144..704)
                     /locus_tag="PLT08_02455"
                     /inference="COORDINATES: protein motif:HMM:NF018949.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NnrU family protein"
                     /protein_id="HNE03353.1"
                     /translation="MNLFIILLSLALWGVVHSFLASHLAKDLFGMKRLYRLLYNIFSL
                     VSFAPILYLVATLEDAALYQIPSPWSWVMRGGQLLAALLLLVAVLQTDTLSFVGLRQL
                     FEEEKTGQLVTRGLYKVVRHPLYLFSLLFLWLSPSMSINSLVFNIGVTAYFIIGAYFE
                     ERKLLRDFGEAYADYKRRTSFLIPGL"
     gene            complement(808..3375)
                     /locus_tag="PLT08_02460"
     CDS             complement(808..3375)
                     /locus_tag="PLT08_02460"
                     /inference="COORDINATES: protein motif:HMM:NF014044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF87 domain-containing protein"
                     /protein_id="HNE03354.1"
                     /translation="MSDNTFYLGRLFDPKTAKLTSTDINYDSADLTTHAVVTGMTGSG
                     KTGLCISLLEEAALNGVPAIIIDPKGDLTNLLLHFPELLPKDFQPWIDPEVVRRANKT
                     PEQASQEAAMAWRNGLQEWGMTPERILDLKNSVDFAIYTPGSDSGIPVSVLSSLDAPG
                     LDWDSNREVLRERITSTVTALLGLVGMNDLDPIRSREHILLSNIFEEHWSQGNGIDLT
                     ELILQTQNPPFDKLGAFPVDTFFPPKDRMALAMTLNNILASPAFEAWREGQALDVQSL
                     LFKEDGTPRHSIFYLAHLSDGERMFFTTLLLSAVETWMRTQKGSAALRALLYMDEIYG
                     YLPPTANPPSKLPLLRMLKQARAFGLGILLATQNPVDLDYKALSNTGTWFIGKLQTER
                     DKNRLLDGLESLAGGFSRAEMDKLISSLGKRVFVMNNVHEKNPVLFQTRWAMNFLAGP
                     MTRTQIRDLNALVGAGEVAAPQPKPKPVSAPKVTAAPPPVDHVQPISVPSAPAQPKKT
                     PTRSTSSSVLESSNGGNLTKPPVPAGIREYYLPQNYSMPEAFSAAGKPQAYGANIQGV
                     VYKPVLLASAQIRVLDRKYGVDVEVTKTALVDSLDRRGIVRWEDFTYAGPSLDKLESG
                     PAPSSKYGSIEAPLNDSKLMTALQKDFTDWVFRNSSVEARANVALKVFAGPDVSQADF
                     MKACADTAREARDTEIAKKTSAIEKKIKTLEDKLGREERELREDEAELQNRNIESGAN
                     LLELGASVLGLTRKKSVTTQFTKHRLAQSAKAEVQESQETISKLTKDLNDLIREREQV
                     IREISDKWGRIVSESNEVTINPKKTDIYVNIFGIAWKPYYLIQAGGETTELAAFGAD"
     assembly_gap    3647..3746
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<3747..4718)
                     /locus_tag="PLT08_02465"
     CDS             complement(<3747..4718)
                     /locus_tag="PLT08_02465"
                     /inference="COORDINATES: protein motif:HMM:NF039823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5667 domain-containing protein"
                     /protein_id="HNE03355.1"
                     /translation="MTNLFDALEFCLREIEEGGTIETALARFPEHAEELRPILETSVK
                     AREMAVPEPSPNVVRRSRALVLQRAAELRESRVAPRSDSRRGFSLFQRLALSFALVAV
                     FLMSGGGLLSASASALPGEQLYTVKRGWENVRLFFIFDTQARALLEHEFENERLHEAN
                     ELILEGRHEVIQFAGVFMQVNGLTYVSGLPVTLPATIPLPANGDAVVVSGRTNSQGFV
                     EVINLEFLPAGSVVPVGKPIEVETESDTEQATGQTPTPSTGTSSGSTNEATPTPVKEY
                     EVKGTLEAVSTTSLVVTGMTVYLENSKIDGQMCVGVEVEVKGYYAQDG"
     gene            complement(4711..5259)
                     /locus_tag="PLT08_02470"
     CDS             complement(4711..5259)
                     /locus_tag="PLT08_02470"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HNE03356.1"
                     /translation="MTTINGQQNISRLDGLDSKAIGAVYDQYFSEVYRFVLYRVGDQS
                     LAEDIASDVFIRLLEAIKVGRGPDSNLKGWLIGTASHVVTDHFRKKYRRPEDELSDSL
                     PGLEPSPASEVDQREQNRLVRSAYKKLTAEQQDVLALRFGQGYSLEETAARMDKNINA
                     VKALQFRALAALQREIGEVDYD"
     gene            complement(5288..6751)
                     /locus_tag="PLT08_02475"
     CDS             complement(5288..6751)
                     /locus_tag="PLT08_02475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE03357.1"
                     /translation="MQKTLRTSLLLFIAFFIGMAVIQFSTPNLPDNDGFYHIKMAWLM
                     RTEGLTPEFIWLPKSILNQNDFYDHHFLYHVALIPFTFGDLRVGAKLASVTFSALAFL
                     AVWVLFDRQKIPFAWLWALALLGISDAFLFRMSIPRAQSLSLGILALGLLWMFEGKYK
                     HLAVLSFFYVWTYNAFPLMIALAGLYTLAILLTEKRLDLRPVLWTSFGIFAGLVLNPY
                     FPKNLTFTYHHFLSKLQLGESIKVGNEWYPYDTAQVLSNSLLALVALFSGSMALGFSG
                     RKMTVQTVTAFLIALFFGIMMFNARRFVEYFPPFALIFSASAWAPLFDNDGQPTQSWM
                     QKIPAGILALAILAGMVKSIPAVQHQLGTSKPYDLYAGASFWLLQNSEKGELIFQTDW
                     DDFPRLFYQNTYNTYLAGLDPTYFQLYNPELYNEWVSITQGDVANPSKNIFSHFGSRF
                     VISDLNHGDFIQQAESDPNMREVYRDGQSVLFEIIEP"
BASE COUNT         1858 a         1628 c         1690 g         1680 t
ORIGIN      
        1 ccgtccacct aaacgctttc aaaggcgggt cgggcacgcg gaggcaagat tatcatgcct
       61 gaaatagggt gtcaagattt tatgaaaaat ggtccgttag acagtagttg acggatgtcc
      121 aactaatgtc aatggtctgt tctttacaag ccagggatta gaaatgaggt gcggcgttta
      181 tagtctgcat aggcttcgcc aaaatcacgc aggagtttac gctcttcaaa ataagcaccg
      241 ataatgaaat atgccgtcac accaatgttg aacaccaacg aattgatact catcgacggg
      301 cttagccata aaaacagcaa gctaaataag taaagcggat gccgtacaac tttgtacaaa
      361 ccgcgcgtca caagttgacc ggttttctct tcttcaaata attgacgcaa accaacaaag
      421 gaaagtgtat ctgtttgtaa tacagccact agaagtaaca acgccgcaag taactgtccg
      481 ccacgcatga cccagctcca cggggatggg atttggtaga gagccgcatc ttccaatgtt
      541 gctaccagat agagaatggg agcaaaactc accaaagaaa agatgttata gagcaagcgg
      601 taaagacgct tcatcccgaa caggtctttg gcgagatgtg aagcaaggaa agaatgtact
      661 acgccccata aagcaagtga aagcaggata ataaataggt tcatgtattc ttagatgcag
      721 caagagctgg tttcgttaca aagaatagat agaaaaagcg gatgatttga atatcatccg
      781 cttttttata ctctacaaaa ttaagggtta atctgctcca aaggctgcca gttcggtcgt
      841 ctcaccgccg gcttggatga gatagtaagg cttccaagca atcccaaaga tgttgacgta
      901 gatatcggtc ttcttcgggt tgatggtcac ttcatttgac tcactcacga tcctgcccca
      961 cttgtcgcta atttcgcgaa ttacttgctc gcgttcgcgg atgaggtcat tcaagtcttt
     1021 ggtcaacttg gaaatggtct cctgcgattc ttgtacttca gcctttgcac tttgagccaa
     1081 acggtgtttg gtaaattgag tggtaacgct cttctttctc gtcagcccca agacactagc
     1141 gcccaactct aaaagattcg ctccagactc gatattgcga ttctgcaatt cggcttcgtc
     1201 ttcgcgcagt tcacgttctt ctctgcctaa tttgtcttcc aacgttttaa tcttcttctc
     1261 aatggcagag gtcttcttgg cgatttcagt atcgcgggct tcacgagcgg tgtcggcaca
     1321 agctttcatg aaatctgcct gactcacatc aggacctgca aatactttga gcgcgacatt
     1381 agcacgagcc tcaaccgatg aatttcggaa gacccaatcg gtaaaatctt tttgcaaagc
     1441 agtcattaac tttgagtcgt tcaaaggcgc ttcgatggaa ccgtatttgg aggaaggagc
     1501 gggtccgctt tcgagtttgt cgagcgaagg accggcatag gtgaaatctt cccaacggac
     1561 aatgccgcga cggtcgaggc tatctacgag agcggtctta gtcacttcta catccacacc
     1621 gtatttgcgg tctaagacgc gaatctgggc agatgccaac aagaccggtt tatagaccac
     1681 gccttgaata ttggcaccat aagcttgggg ttttccggcg gcgctaaagg cttcgggcat
     1741 gctgtagttt tgcggcaaat aatattcgcg gataccagca ggaacgggcg gcttggttag
     1801 attgccgcca ttggaagatt ccaagacaga agacgacgtg ctgcgtgtgg gagtcttttt
     1861 aggctgtgca ggcgcagaag ggactgagat cggttggacg tggtcaacag gcggcggggc
     1921 ggctgtcact ttgggagcag agacaggctt cggcttgggt tgaggcgcag ccacttcccc
     1981 agcaccgacc aaggcgttca agtcgcgaat ctgagtgcgg gtcatgggac ctgcgaggaa
     2041 gttcatcgcc caacgcgttt ggaaaagcac gggattcttt tcgtgtacat tgttcatgac
     2101 gaaaacgcgc ttgccgaggg atgagatgag cttgtccatt tcggcgcggg agaaaccgcc
     2161 cgccaaactt tcgagcccat cgagcagacg gttcttatcg cgctcggtct ggagcttgcc
     2221 aatgaaccat gtgcctgtat ttgagagtgc cttgtaatca agatcgaccg ggttttgagt
     2281 agcaagcaaa atgcccaagc caaacgcacg agcctgcttc aacatgcgaa gcaatggaag
     2341 cttggaagga ggattggcag tgggaggaag gtaaccgtag atctcatcca tataaagaag
     2401 ggcacgcaaa gcagcggagc ctttttgtgt gcgcatccag gtttcaacgg cagagagcaa
     2461 caaggttgtg aagaacatac gctcgccatc ggaaagatga gcaaggtaga aaatgctgtg
     2521 acgcggagtg ccatcttcct tgaagagcaa ggactgcaca tccaatgcct gaccttcacg
     2581 ccatgcttca aatgcaggcg aagcaaggat gttgttgagg gtcatcgcca atgccatgcg
     2641 gtctttgggc gggaagaaag tatcgactgg gaaggcgccc aatttatcga aaggcgggtt
     2701 ttgtgtctgc aagatgagct cggttaggtc gatgccattg ccttgcgacc aatgttcttc
     2761 aaagatattc gaaagcaaaa tatgttcacg cgagcggatc gggtcgagat cattcatacc
     2821 gaccaagcct agcaaggctg taaccgtgct ggtgatgcgt tcgcgcaaca cttcgcggtt
     2881 ggagtcccaa tctaaaccgg gggcatcgag cgaagaaagc acactgacag gaatacccga
     2941 gtcagagccg ggtgtataaa tagcaaaatc aactgaattc ttgaggtcga gaattcgttc
     3001 gggagtcatc ccccattctt gcagtccatt gcgccacgcc atggcagctt cttgcgaggc
     3061 ttgctcaggt gttttgtttg cacggcgcac aacttcggga tcgatccacg gctgaaaatc
     3121 ttttggcaat aattcaggaa aatgcaggag cagattggtc agatcccctt tggggtcgat
     3181 gatgatggca ggcacaccat tgagcgctgc ttcttcaaga agcgaaatgc ataagcccgt
     3241 tttcccagaa ccggtcatac cggtgacaac tgcatgggtt gtcaggtcgg ctgagtcata
     3301 gttaatatcg gttgaagtga gcttggcagt tttcgggtca aacagacgcc caagataaaa
     3361 ggtgttgtcg ctcatgtaat ctctcctatg gatataagac ttttgaagtc ttgaagttgt
     3421 attgattaga acgaacgtgc tagatgttta atttaaccga ttttatgcca aaaggcaaat
     3481 gattgcgaaa gaaaaactgc gaggggtttc ctcgcagttt ggtcttaatc gttatcgtcg
     3541 ccgccgccac cgtctttacc actgttccca ccatgatcat cccctgagtc atcgccgttt
     3601 gagttgcttt ctccagagct attcgaacca gactcgttat tactacnnnn nnnnnnnnnn
     3661 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3721 nnnnnnnnnn nnnnnnnnnn nnnnnnaccg tcttgagcgt aatagccctt cacttccact
     3781 tcaacaccca cacacatctg cccatcgatt ttggaatttt caagatacac cgtcatacca
     3841 gtgacgacga gagatgtagt agataccgct tcaagcgtgc ctttgacttc atattccttc
     3901 accggagtag gtgtcgcctc attcgtggac ccagaactgg tacctgttga aggagttggg
     3961 gtttgccctg tagcttgttc tgtatctgac tccgtttcca cttcaattgg ttttccaacc
     4021 ggcacgactg agccggcggg taaaaactcc aagttgataa cttctacaaa gccttgtgaa
     4081 ttcgtgcgtc cgctgacgac cacagcatct ccattggcag ggaggggaat agtagcaggc
     4141 aaggtcacgg gcaagccaga gacgtatgtc aacccgttca cttgcatgaa aacacccgcg
     4201 aactgaatca cttcgtgacg tccttcaaga atcaattcat tggcttcatg caaccgttca
     4261 ttttcaaatt catgttccaa caaagcgcgg gcttgggtgt caaaaataaa gaagagacgt
     4321 acattttccc agccgcgttt gactgtatac aattgctctc ctggcaacgc cgaagcagat
     4381 gcgctcagta aaccgccgcc actcataagg aagactgcca ccaatgcaaa agaaagcgcc
     4441 aagcgctgaa agagtgaaaa gcctcttcga gagtcactgc gtggagcaac tctcgattcg
     4501 cgtaattctg cagcacgttg taacaccaag gcacgagaac gtcgcaccac atttggcgaa
     4561 ggttcaggga ctgccatctc ccgggctttg accgaagttt ccaaaatcgg gcgaagttcc
     4621 tcggcatgtt cggggaagcg cgccagagcg gtttctatcg ttccgccttc ttcgatctca
     4681 cgcagacaaa attcaagagc atcaaataaa ttagtcataa tctacctcgc caatctctcg
     4741 ttgaagcgca gccagcgccc ggaattggag agctttcaca gcgtttatat ttttatccat
     4801 tcgcgcagcg gtttcctcga gcgaatagcc ctgcccaaaa cgtaatgcaa ggacatcctg
     4861 ttgttcagcg gtcaatttct tatatgcgct tcgcaccaaa cgattctgtt cgcgttgatc
     4921 cacttcggaa gcagggcttg gttcaagccc tggcaatgaa tcggagagtt catcctctgg
     4981 acggcggtac ttctttcgga agtggtccgt caccacatgt gaggcggtgc cgatcaacca
     5041 acctttgaga ttggagtctg gtccgcgccc aactttgatc gcttccaaca agcggatgaa
     5101 cacatcgctg gcaatatctt ctgccaaact ttggtcacct acacgataca acacgaagcg
     5161 atagacttca gaaaaatact ggtcatacac ggccccaatg gctttagagt ccaaaccatc
     5221 caggcggctg atgttttgct gcccatttat tgttgtcatc tttgatgttc catacaacat
     5281 aatggtatca cggctcgata atttcaaaca atacagactg cccatcgcga tagacttcgc
     5341 gcatgttggg atcactctct gcttgctgaa taaaatcgcc atggttgagg tcagagatga
     5401 caaaacgtga cccaaaatga gaaaagatat ttttagaagg gtttgccaca tccccttgtg
     5461 taatgctcac ccactcattg tatagctctg ggttatagag ctgaaaatat gtggggtcga
     5521 gcccggcaag ataggtgttg taggtgttct gatagaacaa acgtggaaag tcatcccagt
     5581 cggtttgaaa gatcagttct cctttttctg aattctgtaa cagccaaaaa gatgcacctg
     5641 cgtacaggtc atagggtttg gatgttccca attggtgctg caccgcaggg attgatttca
     5701 ccatgccagc aaggattgcg agagccagaa tcccagctgg aatcttttgc atccagcttt
     5761 gagtcggttg accatcatta tcaaagagcg gcgcccaagc ggatgctgaa aatattaatg
     5821 caaatggcgg gaagtactcc acaaagcgac gagcattgaa catcatgatt ccgaagaata
     5881 aagcaatcaa aaatgcagtt actgtttgta ccgtcatttt gcgacctgaa aaccctagcg
     5941 ccatacttcc actaaacagg gcgaccagtg ccaataagga attgctcaat acctgggctg
     6001 tatcgtatgg ataccactca ttgccaacct tgatcgattc gcctagttgc aacttagata
     6061 agaaatgatg ataggtaaag gtcaggttct tgggaaagta ggggttgagc accaacccgg
     6121 cgaaaatgcc gaagctggtc cacaagaccg ggcggaggtc gagccgtttt tccgtcaaaa
     6181 gaatagcaag ggtgtacaaa ccagctaaag caatcatcaa tgggaaagcg ttgtacgtcc
     6241 aaacataaaa aaaggaaagc acagccagat gtttatattt gccttcaaac atccataaca
     6301 gaccgagcgc caagataccg agtgaaaggg attgcgcccg cggaatggac atgcgaaaca
     6361 aaaaagcatc tgaaatgccc aacaaagcca gcgcccacaa ccatgcaaaa ggaattttct
     6421 ggcgatcaaa caatacccac acagcaagaa aagcgagcgc agaaaatgta acacttgcaa
     6481 gtttggcgcc cacgcgcaga tcaccaaatg taaaagggat aagagctaca tgatagagaa
     6541 aatggtgatc gtaaaaatcg ttctggttta agatgctttt aggaagccag ataaactcgg
     6601 gagtcaagcc ttcggtgcgc atgagccaag ccattttgat atggtagaag ccatcgttat
     6661 ctggcaggtt tggcgtggaa aattgaataa ccgccatgcc aataaaaaaa gcaatgaata
     6721 aaagcagtga agttcgaagt gttttttgca tagaggtctt ttttatatca agtcgcccgg
     6781 gagaaacctg ccgggcgact tttctattcc ttggctagtg aaaactaatg gtcatcacta
     6841 ccgttcgaat tgccgccatg gtcgtcatca tcgtcgccat tgctgttgcc attcgagttg
     6901 ccattatcat cgtcatcatc gccgtttgag ttaccgccat ggtcatcatc atttgt
//
LOCUS       DAOQJE010000056         6918 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_6080_length_6918_cov_6.354364, whole genome shotgun sequence.
ACCESSION   DAOQJE010000056 DAOQJE010000000
VERSION     DAOQJE010000056.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 6918)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6918)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6918
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6080_length_6918_cov_6.354364"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..782
                     /locus_tag="PLT08_02480"
     CDS             <1..782
                     /locus_tag="PLT08_02480"
                     /inference="COORDINATES: protein motif:HMM:NF024117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HNE03358.1"
                     /translation="RRFELRYLQHLAYPAIETEPALENEKHQREGEYFHRLVQQYFIG
                     IPEKQLAKTANTDNLERWWNNFIGDTDLRGLKDLGGLRTEVTLSAPLGSFRLIAKYDL
                     IANDGKKFYIYDWKTYRKRPKNEFLAIRLQTRVYRALLVQAGAFLNGGKPIAPEQIEM
                     VYWFSDFPNAPARFPYQADQFKRDWDAITRYAEEITLRQAQGGASSYPKTEDVNRCLY
                     CPYRSYCNRGVHAGDALDVELEAEAEELFDINFEQIGEIEF"
     gene            complement(819..3428)
                     /locus_tag="PLT08_02485"
     CDS             complement(819..3428)
                     /locus_tag="PLT08_02485"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HNE03359.1"
                     /translation="MKAKTSTRLFLILAIFLVAVPTSVFASTAPPVDMFQLPWEQGKA
                     WYPYDGLDNGLKRSSTSPHNYNMGGALDFAPRAKMTVGEDTSNDWVTAAAAGTVYQVG
                     SCYVKIDHGNGWITEYWHLDKIQVTTGTKVSRNQRLAIIHNNKTQQVCIGNEYPGPHL
                     HFVFRPKVLDTRFAGWTVNYNLFTNITTFSKSGQTVGRLQPLMNIPDLQIANRGILDW
                     NINYEGNIDAYRQERWSLMLVDEAKFEINVTPKGIGLVPVIVLLDASGNEITRASGTL
                     STTQPAGFYYVDVRSEAGTGYYNIIATKSESGGSTVTPSPTASETGTFTPTVTATLNG
                     SETPTATGTLTGSETPTATGTLTGSETPTATETPIGFATHTPTSTGVPDTSTPEPSTD
                     TPTPTLTATATFTPTSTPDFEQTAIAATQTSNAATLTAIANEATQTAISASETANAAT
                     LTAIANGATQTAIAATQTFDAQTQTAIVGTLTATADAATQTAIVGTQTAIAGTLTATA
                     DAATQTSIAITQTANVGTQTAIAATQTSDAAMQTAIAGTLTAQPGIQTSIAGTATANA
                     LTMTASASTFTPSPTSTATVVPTPVGPYVLVDVIQPTLTLNQTSLVNVSLNNVPAEGY
                     TSTEFVCTYDPAVIEVSNILAAELFGSDPASALSGPQNGQFILAIAGSNGKKATSSGT
                     ALTFSVRGLQVGQSTVQCTARVSMGQGNLEPIAYIPDVVTVIDVSVTPTPTTGGVTGQ
                     VIASKPVTIRLYDAQNTLVISQVANPDGTFSLQVPAGTYTIIASADGFLNAQGFLTIT
                     NGGIVTKPTINLPAGDIDGNSVIDQFDALTIGMNYSGSLPAAADLNNDGVINVLDLEL
                     LAANYRLSGALAW"
     gene            complement(3468..4130)
                     /locus_tag="PLT08_02490"
     CDS             complement(3468..4130)
                     /locus_tag="PLT08_02490"
                     /inference="COORDINATES: protein motif:HMM:NF013156.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cohesin domain-containing protein"
                     /protein_id="HNE03360.1"
                     /translation="MKKFALTLIALICLFVVMPVKAQSAESIWIAASTNSFKTGETVI
                     VTVNGLSATPIQGLTFQIKFDPACLQPVNAASPIAGMNGLSLPQISGVVDASFASTTP
                     QVANGVLAEVRFITLGACQTNLTLESAALVIKNESGFAAPLAGVNLGEKAVALAISAE
                     KGTSADAPLLGTPLALGTEPENSGSSLPAGTTIILAVIGVLLIVGIVVLVRILRGENS
                     RG"
     gene            complement(4193..5440)
                     /locus_tag="PLT08_02495"
     CDS             complement(4193..5440)
                     /locus_tag="PLT08_02495"
                     /inference="COORDINATES: protein motif:HMM:NF013156.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase regulatory-like
                     domain-containing protein"
                     /protein_id="HNE03361.1"
                     /translation="MKFKNLFRVAMLFATIISVFGTGAQAYAQGNSTNYLGAQIIIRS
                     TTFWDAVFNGTVNANRYERWSLQLIEPENFTISIITTSGTLTPDIYLLDASENQIASA
                     TGTLSSASLTTNQLAGDYFIQVQPHSGDGTYLMEITRTEQVVEPTASVVLNPAVIPVG
                     GTSTGTLMLSNIPSGGYASAEFTCTYNPALVQISNITDAGLFGSDPAVAVNGPVDGSF
                     VFAVAGSNSQRATSDGAVFTFTVTGLAAGQADIACTVRVSAGGSLSTITSTPASLTIE
                     EVTATLNGTVNATKTVTVTLYKQDTTVEATTTVDTSGNFTITAPAGTYTVVASAPGFL
                     KAQGSPVLTAGATKTMQTISLLAGDIDGNDVIDQFDALTIGMNYNLIEPTAADLNNDG
                     TINVLDLELLAANYRASGASAWQ"
     gene            complement(5713..6597)
                     /locus_tag="PLT08_02500"
     CDS             complement(5713..6597)
                     /locus_tag="PLT08_02500"
                     /inference="COORDINATES: protein motif:HMM:NF021507.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiester glycosidase family protein"
                     /protein_id="HNE03362.1"
                     /translation="MRAWIKSTWFWLGIVALFFIAVGVYVISMLGRPPAFPTKYELYP
                     GVTYHRVVRYFPHAMIAHVVVIDRKEAKASFLVTPPDQVEGGTVKARTTSEFLKEFGV
                     QIAINGDGFSPWWSRSPVDYYPHQGDPVTPLGFAAAFGKIYSDGLLQPTQEPTLYISR
                     RNELSFNRVPGKLQHAISGDRMLLLSGEVVPDLDDTTLQPRTAIGTNRNGRYVILVVV
                     DGRQPFYSTGATFKELAELMKAYDAFFAMALDGGGSSTLVVQKKNGGTQILNSPIDNY
                     IPGRERPVANHIGVSIAP"
     gene            complement(6682..>6918)
                     /locus_tag="PLT08_02505"
     CDS             complement(6682..>6918)
                     /locus_tag="PLT08_02505"
                     /inference="COORDINATES: protein motif:HMM:NF018798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1385 domain-containing protein"
                     /protein_id="HNE03363.1"
                     /translation="MPIVWRLVTRVLFIPILAGIAVEYIRWTANHLNNPIVQWLIKPN
                     LALQLLTTRPPDLEMLEVAIASFQAMRKAEAETA"
BASE COUNT         1780 a         1661 c         1800 g         1677 t
ORIGIN      
        1 cgcgccgttt tgagctgcgt tatcttcagc atttggcgta tcccgccatt gaaaccgagc
       61 ccgcacttga aaacgagaaa caccagcgcg aaggtgaata ttttcatcgg ctggtgcaac
      121 agtatttcat cggcattcca gaaaaacaac ttgcgaaaac agctaacaca gataaccttg
      181 aaagatggtg gaacaatttt ataggtgata cagacctccg aggtcttaaa gacctcggag
      241 gtctaaggac tgaggtaact ttatccgccc ctttaggctc tttccgcctc atcgccaaat
      301 acgatctaat cgccaacgat ggaaaaaagt tttacatcta cgattggaag acctatcgca
      361 aacgcccaaa aaatgagttt cttgccatcc gtttgcaaac tcgcgtctac cgcgcgttgt
      421 tagtgcaagc gggcgcgttt ctcaacggtg gcaagcctat tgcccccgaa cagatcgaga
      481 tggtgtattg gttctccgat tttcccaacg ccccggctcg attcccatac caagccgatc
      541 agttcaaacg agattgggat gccatcacac gctatgcaga ggaaataacc cttcgacaag
      601 ctcagggcgg cgcctcttcc tacccgaaaa ctgaagatgt caaccgctgc ctttactgcc
      661 cttatagatc atactgcaac cgcggagtcc atgccggaga tgcgcttgat gtggaattag
      721 aggcagaagc cgaagagttg tttgatatca actttgaaca gattggggag atcgagtttt
      781 aaaacaaaaa aggcgaccct gacgggtcgc cttttttgtt accacgcaag tgcgccagat
      841 aagcgatagt tcgctgccag tagctcaagg tcgaggacgt tgatcacgcc gtcgttgttg
      901 aggtcggctg cggcggggag agagccgctg tagttcatcc cgatcgtgag tgcatcgaac
      961 tggtcaatca cgctgttgcc gtcaatatca ccagctggca ggttgatggt tggcttggtg
     1021 acgatgcctc cgttggtgat tgttaggaaa ccttgggcat tcaggaagcc atcagcagaa
     1081 gctatgatgg tgtaggtgcc agcagggact tgtaagctga aggttccatc tgggttggca
     1141 acctgtgaga tgacaagtgt gttttgagca tcatacaagc ggatggtgac tggcttgcta
     1201 gcaatcactt gcccagtcac gccgcctgtc gttggtgtgg gggtaacgct cacatcgata
     1261 acggtgacga catctggaat gtaagcaatt ggctcaaggt tgccctgccc catcgaaacg
     1321 cgagctgtac attgaacagt gctttgacct acttgcaatc cgcgtaccga gaaggttaga
     1381 gcggtgccgc tggaggtggc tttcttgccg ttactgcctg caatcgcaag gatgaactgt
     1441 ccattttgag gtccgctcaa agcagaagcg gggtctgaac cgaagagttc tgcagcgagg
     1501 atattgctga cttcaatgac cgcagggtcg taagtacaaa caaactctgt actggtatat
     1561 ccttcggcag ggacattgtt caagctgaca ttgacaagac tggtttggtt aagtgtgagg
     1621 gtcggttgaa tgacatctac caacacatag ggtcccacag gggtgggtac aaccgtcgcc
     1681 gtacttgtcg gcgaaggtgt aaaggtggac gcagatgcag tcattgtcag tgcattggct
     1741 gtggcggtac ccgcgatcga agtttgaatt ccaggctgag ccgttaacgt gcctgcgatg
     1801 gcagtttgca tggctgcatc agaagtttgt gtagcggcaa tagcagtctg cgtgcccaca
     1861 tttgcagttt gagttatagc gatagaagtt tgcgtagctg catctgcagt tgctgtgagt
     1921 gttccagcaa tcgcggtttg agtcccgaca atggcagttt gtgtagcggc atcggctgtg
     1981 gctgtgagcg tccctacaat ggcggtttga gtttgggcat caaaagtttg tgttgctgca
     2041 atagcagttt gagtagcgcc attggcaatt gccgttagcg ttgcggcgtt tgctgtttcg
     2101 ctggctgaaa ttgcggtttg ggttgcttcg ttcgcaatgg cagttaacgt ggctgcattt
     2161 gatgtttgcg tcgccgcaat cgctgtctgc tcaaaatcgg gtgtagaagt tggtgtaaat
     2221 gtagcagtag ccgttagggt tggcgtggga gtatctgtgc tgggttcagg ggtgcttgta
     2281 tctggaacac cagtagaagt tggggtatga gtcgcaaaac caattggggt ctcagtagcc
     2341 gtaggggttt cagaaccagt gagcgtaccc gtcgcagttg gagtttcaga gccggttaag
     2401 gttccggttg cggttggggt ctctgagccg ttcaaggttg cagttacggt tggagtgaat
     2461 gtccctgttt ctgaagccgt cggtgatggc gtgacggtgg agccgcctga ttcgcttttg
     2521 gtggcaataa tattgtagta ccccgtaccc gcctcagaac ggacatcgac ataatagaaa
     2581 ccagcaggct gagtggtgct caatgtacca ctcgcccgtg taatctcgtt tccacttgcg
     2641 tctaacaaaa caatgaccgg gacaagccca attcctttcg gggtgacgtt aatctcaaac
     2701 tttgcttcat cgaccagcat aagagaccaa cgttcctgcc gatatgcatc gatattgcct
     2761 tcgtaattga tattccaatc aagaatgcca cgattcgcga tctgcaggtc aggaatgttc
     2821 atcaaaggct gaagcctgcc caccgtctgc ccgctctttg agaaggtggt gatattcgtg
     2881 aacaagttgt agttgaccgt ccaaccggca aagcgcgtat cgagtacttt gggtcggaat
     2941 acaaaatgta aatgtggccc cggatactca ttcccaatac agacttgttg ggttttgtta
     3001 ttgtgaataa tcgccaagcg ttgatttcgg ctcaccttgg tgccagtggt cacttgaatc
     3061 ttgtccaagt gccaatattc ggtgatccag ccattgccat gatcgatctt gacataacaa
     3121 gaaccgactt gatagaccgt tccagctgcc gcggcagtga cccagtcatt tgaggtatct
     3181 tcccctaccg tcatctttgc acgaggagca aagtctaacg cgccgcccat attgtagtta
     3241 tgtgggctgg tagaagaacg cttgagtcca ttatccagtc catcgtatgg ataccaagct
     3301 ttgccttgtt cccaaggcag ttggaacata tccacgggag gtgccgtgct cgcaaataca
     3361 cttgttggaa cagcaacaag gaagatcgcg aggatcaaaa atagtctggt agatgtctta
     3421 gctttcataa tggcaccgtg ccctatcaaa acggactgtg gataaaatta accgcgcgaa
     3481 ttttcaccgc gcaaaatgcg gacaagcaca acaataccga cgatgagaag cacaccaatc
     3541 acagcaagaa tgatggtggt gccggcaggt agagaagaac cggaattttc aggttcggtg
     3601 cctaacgcca gtggagtgcc caacaaaggc gcatcggcag aggtaccctt ctcagcgctg
     3661 atggcgagag ccacggcttt ttctccaagg tttacaccag ccaaaggagc cgcaaaccca
     3721 gattcattct taataacgag cgcagcactc tctaacgtta aattggtctg acaggcacca
     3781 agagtaataa aacgtacttc agctaacacg ccattcgcaa cttgaggcgt ggtacttgca
     3841 aaggaagcat ccacaacccc gctgatctgt ggaagggaaa gcccgttcat gccagcaata
     3901 gggctggcag cgttgacagg ttgaaggcat gccgggtcaa acttgatctg aaaggtcagc
     3961 ccttgaatcg gggtggcaga aagcccattg accgtaacga tcactgtttc accagtttta
     4021 aaactattcg tgctggcagc aatccaaatg gattctgcag attgagcctt tacaggcata
     4081 acaacaaaaa gacagataag tgcaatgagg gtcagtgcaa attttttcat tgaagtctcc
     4141 atggagggtg caccgatgat gaatcggtgc accctttgca ttgccagggg atttattgcc
     4201 aggcacttgc gccagaagcg cggtagttcg ctgccagtaa ttcaaggtca agaacattga
     4261 tggtgccatc attgttcaag tctgcagcgg tgggttcaat aaggttgtag ttcataccaa
     4321 tcgtaagagc atcaaactga tcgatcacat cattgccatc gatatcaccg gcgagaagag
     4381 agatcgtctg catggtcttg gttgcaccag cggtgaggac cgggctgccc tgggctttga
     4441 ggaacccggg agccgatgcg acaacggtgt aagtccccgc tggagccgtg atcgtaaaat
     4501 ttccgcttgt gtccacagta gtggtggctt ctacagtcgt atcctgctta tagagagtca
     4561 ccgtcacagt cttggtagcg ttgaccgtac cattgagagt agcagtcact tcttcaatcg
     4621 tgagtgaagc cggggtggac gtaatggtgc tcaacgaacc gccagctgaa acgcggacgg
     4681 tacaagcaat atctgcttgc ccagccgcaa gaccagtcac cgtaaaggtg aagacagcgc
     4741 catctgaagt agcacgctgg ctattgctgc ctgctaccgc aaacacgaag gaaccatcga
     4801 cgggtccatt aacagcaacg gcggggtcag aaccgaacaa accagcgtcg gtaatattgc
     4861 tgatctgaac caaagcgggg ttataggtac aagtaaattc tgcgctggca tatccgccac
     4921 tggggatatt gctcaacatc aaagtacctg tggaggttcc gccaacagga ataaccgccg
     4981 ggttcaatac cacggatgcg gtcggttcaa ccacctgctc agtgcgggtg atctccatta
     5041 aataagtacc atccccagag tgcggctgga cttgaatgaa gtaatcccct gcaagctgat
     5101 tggtagtcag ggaagcgctt gaaagcgtac ccgttgcaga agcgatctgg ttctcgctgg
     5161 catctaacaa gtagatatcg ggagtgagcg tgccagatgt ggtgatgatc gagatggtga
     5221 agttctcagg ctcgatcaac tgcaatgacc agcgctcata gcgattcgca tttaccgtcc
     5281 cattgaagac tgcgtcccaa aaggtggtgc tgcgaataat gatctgtgcg ccaaggtaat
     5341 tggtgctgtt tccttgcgcg tacgcttgcg cacccgtgcc aaagacactg atgatggttg
     5401 cgaacagcat cgcaacgcga aacaaattct taaatttcat cgtattctcc ttttcgaatc
     5461 aaggtgatat aaggcagaaa gcctacataa caaaaaaggg tatcgtttcg tatttcctcc
     5521 aaactgccaa ccgtggcggt tcaatgatga agacttaaaa gccaattaca ttctattggt
     5581 gcaactgcac aatatgcgtt gcaggtgagt tgccacgata ttgcagaata gttgcgacat
     5641 gtctgcttaa gaatcaagac ccacttcatg tgaagtgggt cttgaaaaat caatttaaaa
     5701 atctattttt aattacggtg caatcgagac accaatatga tttgcaacag gacgttcacg
     5761 ccccggaata tagttatcaa taggtgaatt caatatttgc gttcccccat ttttcttttg
     5821 cacaaccaat gtagaagacc cacctccatc taatgccatg gcaaagaagg catcatatgc
     5881 tttcatcaac tcagccagtt ctttgaatgt ggcaccagta ctataaaacg gctggcgacc
     5941 atccaccact acgagaatca cataacgccc attgcgattc gtaccgatgg ctgtgcgtgg
     6001 ctgtaaagtt gtatcatcca aatctggcac aacctctcca ctaagaagca acatgcggtc
     6061 tcctgaaata gcatgttgca atttccccgg cacgcgatta aacgataatt cgttacgacg
     6121 gctgatgtat agcgttggct cttgtgtagg ttgcagcaac ccatctgagt agatttttcc
     6181 aaatgcggca gcaaacccca agggtgtgac cgggtctccc tgatgcggat aataatccac
     6241 cgggctgcga gaccaccacg gagagaagcc atcgccattg atggcgatct gcaccccgaa
     6301 ctctttcaaa aactcagaag tggtacgcgc ctttacggtt ccaccctcga cctggtctgg
     6361 cggcgtgacc agaaaacttg ccttcgcctc tttccgatca atcacaacca catgcgcgat
     6421 catggcatgt ggaaaataac gcaccacgcg atgataagtc acaccggggt aaagctcata
     6481 ctttgttggg aaagccgggg ggcgccccaa catggagata acatataccc ctacagcgat
     6541 gaagaacaac gctactattc ccagccaaaa ccacgtagat tttatccatg ctctcatgtg
     6601 gaaaatgcta tcaagccaag atgaagaaaa aataaaaaga gacgggcgaa aacccgtctc
     6661 tttctctacg atagatacac tttacgccgt ttcggcttct gccttgcgca tggcttggaa
     6721 ggaagcaatc gccacttcga gcatttccaa atctggcgga cgggtggtga gcagttgcaa
     6781 tgccaaattt ggcttaatca accattgcac aatgggattg ttcaagtgat ttgccgtcca
     6841 gcggatgtat tcgaccgcaa tgcccgccaa aatagggatg aacaaaacac gggtgaccaa
     6901 acgccaaacg atgggcat
//
LOCUS       DAOQJE010000057         6875 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_6133_length_6875_cov_6.361144, whole genome shotgun sequence.
ACCESSION   DAOQJE010000057 DAOQJE010000000
VERSION     DAOQJE010000057.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 6875)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6875)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6875
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6133_length_6875_cov_6.361144"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            76..288
                     /locus_tag="PLT08_02510"
     CDS             76..288
                     /locus_tag="PLT08_02510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE03364.1"
                     /translation="MKCWHCEQEARASCSFCGRFVCKDHASTMSTFITMFVGANNTPK
                     GLAVANVIWCGECEPQPEPISMPELY"
     gene            300..659
                     /locus_tag="PLT08_02515"
     CDS             300..659
                     /locus_tag="PLT08_02515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE03365.1"
                     /translation="MPGLFDRLDKEIKDNQKKDGISPLDIAKLPPAMRKIMRVMLREL
                     QASYPRMCEVVDAMPEQDRLTRTELDESLATLTEQLWLIRIGEKEKAIYKVNLRRKTG
                     STLMAGIWSSLDTKLKR"
     gene            complement(703..2259)
                     /locus_tag="PLT08_02520"
     CDS             complement(703..2259)
                     /locus_tag="PLT08_02520"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012437.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016849 - phosphorus-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0032555 - purine ribonucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HNE03366.1"
                     /translation="MLPESPLAIIESRLRYLLPSEMYAEMWGNPSMEIMIRVHNHLRT
                     LQRIIHDYTSRQIDVSQLKPGQVKTEWQYGTMMFTDLAGFTKLMEANASKGRDGAENL
                     LKELTKYFSTMISIISKSGGELVEFTGDAMLVVFAKKKEKNDDAQRAIRAGLRMQRAM
                     KDFAEIQTPSGIVNLQMRIGIHSGRFLTAEIGTPRRMEHVLLGKDVQKAKLTESKGRN
                     ERVNVSVTAYELAKDSFRFDEGEPDYHLVVDDLTADSLGDYEITPMGRRMASSVLFDL
                     KDKKEVLSNITELLNSIEPLASYIPDPVLSMLVESAADRKIRPDFPTPTIMFVNFIGL
                     PEAADRALPGEERKLALSFSKAFSLINAAVEARGGVLKKVTYHLSGSDIVIYFGVPNA
                     HTNNEMRAASAAVAIREIVESINLKAPTIGGIKPEVYCQIGINMGPAFVAEVGDPRGR
                     REFNVLGDTVNTAARLMNRAQKNQIVLSETVKQSIESKYECTPLGEIELKGKSHPMSL
                     YELTKKKPKE"
     gene            2323..3081
                     /locus_tag="PLT08_02525"
     CDS             2323..3081
                     /locus_tag="PLT08_02525"
                     /inference="COORDINATES: protein motif:HMM:NF012706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HNE03367.1"
                     /translation="MKNKKITLFVVLLIMGILLVACGGGTPPAEETQIVDATQAPVEA
                     TATIQPVSTVDLKLDPANLASDNAKAAVPYLYEGLVRLQDGAVVGALASTFTVSEDGL
                     DYIFDLRTGVTFHDGTLLNADTVVLNFNRWFDPADANRGSGEYAAWVANFGGFKGELT
                     EDGKPKSPVDGIEKQDEFTVIIHLNTPDPDLLTKLTDPAFSIMSASSFAGNDGGTGPY
                     KAASNDGTTITLEPFAGYWDPAAIPSANVEIPAP"
     gene            complement(3133..4275)
                     /locus_tag="PLT08_02530"
     CDS             complement(3133..4275)
                     /locus_tag="PLT08_02530"
                     /inference="COORDINATES: protein motif:HMM:NF013123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DPP IV N-terminal domain-containing protein"
                     /protein_id="HNE03368.1"
                     /translation="MTLFLQKHRHIFTLAGAILLALAVSACGENIDLSGAAGLGNSTD
                     VAATDVVATEETAVVAEAPTATPLPAATGHIIFASNRDGQTDLYMTTPDGAELTRLTT
                     GAAVDNSSPIRLSPDGTKIAFSATLGGNTDVYVFDLATNFTTRITDAQEKDSSPSWSP
                     NSQQLAFESFRDGNIEIYIANADGSNPIRLTNDPAGDSNPIWSPVSNEILFTSNRFGN
                     SDLMLLNLNGTVSTLTTSRAPDNAAAWSPDGNFIAYQSFSGDISNICIIGRDGLNQVC
                     PSIPAEYGTPVWSPNGTQIAASTLSNGVYSISLFNSRDGSSQQISQPGIEPRGTPAWS
                     PDGLRLVLQTQADGDMELFSVFVQTNEFTRITTIAGYDGEPTWIVQ"
     assembly_gap    4379..4478
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(4571..5485)
                     /locus_tag="PLT08_02535"
     CDS             complement(4571..5485)
                     /locus_tag="PLT08_02535"
                     /inference="COORDINATES: protein motif:HMM:NF013736.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HNE03369.1"
                     /translation="MSQSQRTVISSTSPLKKIMHAVRAIEEGDYDPILLSEFMTETGD
                     LGKLAQMLDSMANTILKRNNQLTLLSKVIPIGVSLSAEKDFNRLLESLVEEARTFTNA
                     DAGSLYLVEDDKLRFVISRNTSLKMHMGGTSGTPITFVPVRMYNEDGSENRANIVSYA
                     ALTRQRINIADAYEAEGFDFSGTKAFDENTRYRSKSFLTIPLETKEGKVMGVLQLINA
                     TDPKTKEIVPFQDDTVLEALILLATAALEGYIREAALRSEIAKLRIEIDQSRRAKQVE
                     EITDTAFFRELKTKATEMRSKTKKEDEE"
     gene            5539..6141
                     /gene="nadD"
                     /locus_tag="PLT08_02540"
     CDS             5539..6141
                     /gene="nadD"
                     /locus_tag="PLT08_02540"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: protein motif:HMM:NF000840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HNE03370.1"
                     /translation="MPKRIGLFGGTFDPPHLGHLVLAAEAVSQFKLDTFLWVLTPDPP
                     HKLNTSITPLSHRLPMLQAMIAHDPVFELSRVEIDRPGPHYTIDTVRFLTQQYGDAEI
                     YLLIGGDSLRDLPSWRLSADLVAEVSKIGVMRRPGDFTDLTPIEDKLPGVTSKIHFIE
                     ALLQPISSREIRRRVASGEMYRYYLAPEVYEYIETHHLYR"
     gene            6165..>6875
                     /locus_tag="PLT08_02545"
     CDS             6165..>6875
                     /locus_tag="PLT08_02545"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNE03371.1"
                     /translation="MPIFYDPIFSFISLGHFMVDTFNASRPVLLTYFGLSESQIAFFS
                     TIYIWASALTQPVFGWISDRTGPRWLAAGGVLWMTVFYSLALTLPGNLGLGCLILAAL
                     GSSAFHPVAAVQATLRGREFLKGRETTATSLFFTAGQMGHFVGPIITGLILAYLKIPG
                     MYILPLVSIPIGFALMYQLKDNHPHPQPVKTVISRRNRAGITFILVLMAVATMQSWAQ
                     ANMVNLIPKYIKDLGQGAT"
BASE COUNT         1577 a         1581 c         1718 g         1899 t
ORIGIN      
        1 tatccagacc atgaatggtc gaaggctttg cggaacgttg tgccgttcat cctgaatgca
       61 aaataggagc ctcttatgaa atgctggcat tgtgaacaag aagcccgggc atcctgctca
      121 ttttgtggac gtttcgtttg taaagaccat gcttcgacca tgtccacgtt tattacgatg
      181 ttcgtgggtg cgaataatac ccccaagggg ttggcagtgg ctaatgtgat ctggtgtggt
      241 gaatgtgagc cacaacccga accgatcagt atgcctgagt tgtattaagc gagggaacca
      301 tgcctggtct ttttgatcgc ctcgataaag aaataaaaga caaccaaaag aaagatggca
      361 tctctccatt agatattgcc aagcttccgc ctgccatgcg taagatcatg cgtgtgatgt
      421 tgcgcgagct tcaagcaagc tacccgcgga tgtgcgaagt ggtcgatgcc atgcctgagc
      481 aagaccgcct cacgcgcacc gaattagatg agtctcttgc aacactcacg gaacaactat
      541 ggctgattcg gatcggtgaa aaggaaaagg cgatctataa agttaatttg cgccgtaaaa
      601 cgggcagcac cttaatggca gggatatggt cttcgctcga cacaaaactt aagcgataac
      661 caattctact taaataaaaa cgccgcgatt gcggcgtttt tattattctt ttggtttttt
      721 cttggttagc tcatacaaac tcatggggtg gcttttgccc tttagctcga tctcacccaa
      781 gggcgtacat tcatactttg actcaatcga ttgtttgact gtctctgaga ggacgatctg
      841 attcttttgg gcacggttca ttaagcgagc cgctgtattg accgtatccc ccagcacatt
      901 gaactcacgt ctgccgcgtg ggtcaccaac ttctgccacg aatgccggtc ccatattgat
      961 gccgatctgg caatacactt caggcttgat gcccccgatc gtaggcgctt tgaggttaat
     1021 tgattctaca atttcgcgga ttgcaacagc tgcagaggca gcacgcattt cattgttggt
     1081 gtgagcgttt ggaacgccaa agtatatgac gatatctgaa cctgaaaggt ggtaggtcac
     1141 ttttttcaac accccgccgc gcgcttctac tgctgcattg ataagcgaga aagctttcga
     1201 gaaactgagt gctaatttgc gctcttcgcc aggcagggca cggtctgctg cttctggcaa
     1261 accgataaag ttaacgaaca taatggtagg ggtggggaag tctgggcgga tcttgcggtc
     1321 tgccgcactt tcgacaagca tcgaaaggac agggtcagga atataactgg cgagaggttc
     1381 aatggagttg agcagttcag tgatgttgct caagacttcc tttttgtctt ttaagtcaaa
     1441 tagaacacta cttgccatgc gccgtcccat gggtgtgatt tcgtaatcgc caaggctatc
     1501 cgctgtcagg tcgtccacaa ccaggtgata gtcgggttcg ccttcatcaa agcggaaact
     1561 gtcctttgcc agttcatagg cggtgacaga tacgttcaca cgctcgttgc gtcccttgct
     1621 ttcggtcaat ttggcttttt gcacatcttt accgagcagc acgtgctcca ttcggcgcgg
     1681 tgtaccaatt tctgccgtta gaaacctgcc gctgtggatg ccgatgcgca tttgcaggtt
     1741 gacgattccc gaaggggtct ggatctctgc gaaatctttc attgcccgct gcatacgcaa
     1801 cccggcgcgg atggcgcgct gggcatcatc atttttttcc tttttctttg cgaagaccac
     1861 cagcattgca tcgccagtga actctaccag ttcaccaccc gatttactga tgatcgagat
     1921 catggttgaa aaatattttg ttaattcttt gagaaggttt tccgcaccgt cacgtccctt
     1981 ggatgcgttg gcttccatca gtttggtgaa acctgctagg tcggtgaaca tcatggttcc
     2041 gtactgccat tcggttttca cctgtcccgg ttttaactgg gagacatcga tctgccttga
     2101 tgtgtaatca tggatgatac gttgcagggt tcgcagatga ttgtgaaccc gaatcatgat
     2161 ctccatcgag gggttgcccc acatttcggc gtacatttca gaggggagca gataacgcaa
     2221 gcggctttca ataatggcaa gcggtgactc tggaagcatg atttccatta tacttgaatt
     2281 gaagatgttt actttcaatt ctatctaaag gcagagagaa aaatgaaaaa caaaaaaata
     2341 accttgtttg tagttctctt aataatgggg attttacttg tagcctgcgg cggcggaacg
     2401 cctccagcag aagagacgca aattgtggat gcgacacagg cacctgtcga ggcaaccgct
     2461 actattcaac ccgtctctac cgtggacctg aagcttgatc ctgctaacct agcttcagat
     2521 aacgccaaag ccgctgtgcc ctacctgtat gagggtctgg ttcgtttgca agatggtgct
     2581 gtggttggag cattggcatc cacctttact gtttcagaag atggattgga ctatatattc
     2641 gatctccgaa cgggtgttac tttccatgat gggacattgc ttaacgccga tactgtagtg
     2701 ttgaacttca atcgctggtt tgaccccgcc gatgctaacc gaggctcagg tgaatatgca
     2761 gcttgggtgg caaactttgg cgggttcaaa ggcgagttga cagaagatgg gaaaccgaaa
     2821 tctccagttg acggaatcga gaagcaggac gaatttacag tgattattca cttgaataca
     2881 ccagaccctg acttattgac caagttaacc gacccagctt tttcgatcat gagtgcttcg
     2941 agttttgccg gaaatgatgg tgggactgga ccttataaag cggcatctaa tgatggcact
     3001 accattacat tagaaccatt tgctggttat tgggatccag ccgcaatccc aagcgcgaat
     3061 gtagaaatcc ctgctcctta attttgataa taaaagaccc cgaacattgc gttcggggtc
     3121 ttttattatt ggttattgta caatccaggt tggttcgcca tcatacccgg ctatagtggt
     3181 aatccttgta aattcattgg tttgaacaaa tacagagaag agttccatgt ctccatctgc
     3241 ttgggtttgt aggactaatc gaagcccatc aggtgaccag gctggtgtgc cgcgcggctc
     3301 aattcccggc tggcttattt gttgtgagct accatctcgg ctattgaaaa gggaaatgct
     3361 gtaaacccca tttgagagag tgctggcggc gatttgagta ccattaggag accagactgg
     3421 tgttccatat tccgcaggaa tggatgggca aacttggtta agtccatcgc gcccgatgat
     3481 acagatattc gagatatcgc cgctaaaaga ttggtaggcg ataaagtttc cgtctggcga
     3541 ccaagcggcg gcgttatcag gagcgcggct ggttgtcaga gtagagaccg ttccattgag
     3601 gttgagcaac atcaagtctg aattgccgaa gcggttgctt gtaaaaagga tttcattcga
     3661 gaccggtgac cagattggat tgctgtcccc ggcggggtcg ttggtcaagc gaatgggatt
     3721 ggaaccatcg gcattggcaa tataaatttc aatgttgcca tcacgaaagg attcaaaagc
     3781 tagttgttga ctgttgggag accaggaagg ggaggagtct ttttcttgag cgtctgtgat
     3841 gcgggttgtg aaattggtgg cgaggtcaaa cacgtagaca tcggtgttgc cgccaagcgt
     3901 ggcagaaaag gcaatcttgg ttccatctgg ggagaggcga atggggctgc tgttatccac
     3961 ggcggcgccg gtggtgaggc gggtcagttc tgctccatct ggggtggtca tgtataaatc
     4021 ggtttgacca tcccgattgg aagcaaagat aatgtgaccg gtggcggcgg ggagcggagt
     4081 agctgtgggc gcttctgcaa cgacggcagt ttcttcggtg gcgactacat cagtagccgc
     4141 tacatctgta gagttaccaa gacctgctgc accagacaga tcaatgtttt cgccacaggc
     4201 gcttacggcg agtgcgagca aaatagcgcc agctagggta aaaatgtgtc gatgtttttg
     4261 aaggaaaagg gtcatggatc ttctacccaa tagggctact ttcaggcgga ttaggacttt
     4321 tttactatct ttttgatttc cgtattgtta gtgttcattt atcccccaaa agtgtcccnn
     4381 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4441 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnac tatctttttg atttccgtat
     4501 tgttagtgtt catttatccc ccaaaagtgt ccctagaaca acctcaaaag ttgacaattt
     4561 tgcaaacccc ttactcttcg tcttcctttt tcgtctttga gcgcatttcg gtggctttgg
     4621 tcttcagttc gcggaagaat gccgtgtcgg tgatctcttc tacttgtttg gcgcggcgcg
     4681 actggtcaat ctcaatgcgt agtttggcaa tttcgcttct gagagccgcc tcgcggatat
     4741 agccttctaa agcggcggtg gcgagcaaga tcagcgcttc caaaacggtg tcgtcttgga
     4801 aaggaacgat ctctttggtc tttgggtcgg tggcgttgat caattgcaac acgcccatca
     4861 ccttgccttc tttggtttcg agcggaatgg tcaggaagga tttggaacgg tagcgggtat
     4921 tttcgtcgaa ggcttttgta ccggagaagt cgaaaccttc ggcttcgtag gcatcggcaa
     4981 tgttgatgcg ctggcgggtc aatgccgcat acgaaacaat gttggcgcgg ttctcactgc
     5041 cgtcttcgtt gtacatgcgt actggcacaa atgtaatggg cgtgccgctt gtgccaccca
     5101 tgtgcatttt taaagaagta ttgcgcgaga taacgaagcg cagtttgtca tcttcaacaa
     5161 gatataaaga accggcatcg gcatttgtga aggttcgggc ttcttctact aaagactcca
     5221 gcaaacggtt gaaatctttt tcagcagaaa gtgatacccc gatcggaatg actttgctta
     5281 acaaggtcaa ttgattgttg cgcttcaaaa tggtgttcgc catcgaatcc agcatttgcg
     5341 ccagtttccc cagatcccca gtctcagtca tgaattcgct caagagaatt gggtcataat
     5401 caccctcttc aatggcgcga acggcatgca tgatcttctt aaggggagat gttgaactaa
     5461 taactgtccg ctgcgactgt gacatgtttt tcagtatacc ccaagattct gtgcatgagg
     5521 atgcgatata ctaccgccat gcctaaacgc attggtctct ttggcgggac tttcgatccc
     5581 cctcatctcg gtcatcttgt ccttgcagca gaagcggtct ctcaatttaa gttagataca
     5641 tttctgtggg tgcttacccc agacccgccg cataaattga atacctccat cacgcctctc
     5701 tctcatcgtc ttccgatgtt gcaggcgatg attgctcacg accctgtgtt tgagctgtcg
     5761 cgtgtcgaga tcgatagacc gggtccacat tacacaattg acaccgtccg gtttctgaca
     5821 caacaatatg gggatgctga aatctacttg ctgatcggcg gcgattctct gcgagacctg
     5881 ccaagttggc gcctcagcgc ggacctcgtg gctgaagttt ccaaaattgg cgtgatgcgc
     5941 cgcccaggcg acttcaccga cctcaccccg atagaagata aacttcccgg ggtcacatcc
     6001 aagatccatt ttatcgaagc cttgttgcag cccatctctt cccgtgaaat tcgcaggcgt
     6061 gtggcttctg gggaaatgta tcgttattat cttgcgcctg aagtgtacga gtatattgag
     6121 acccatcatc tctatcgtta gtattcacta tttataattt tcctatgcct attttttacg
     6181 accctatatt ttcttttatc tctctcggtc actttatggt ggataccttc aatgccagcc
     6241 gaccggtgtt gttgacctat ttcggtttga gcgaatcaca aattgccttt ttttctacga
     6301 tctatatttg ggcatctgca ttgacacaac cggtcttcgg ctggatctca gatcggacgg
     6361 gtccgcgttg gttggcagcc ggtggcgttt tgtggatgac cgttttctat tcactcgcgc
     6421 taacgttgcc gggtaatctt ggcttgggtt gtcttatcct tgcggcgttg gggtcttctg
     6481 cgtttcaccc tgtggctgcg gtccaagcca ccttgcgcgg acgtgaattc ttgaaagggc
     6541 gagagacaac cgcaacttca ttatttttca ccgctgggca aatgggacat tttgtaggtc
     6601 ctattattac tgggttgatt ttggcatatt tgaaaattcc tggcatgtat atcttgcctt
     6661 tggtatccat tcccattggc tttgcgttga tgtaccagtt gaaagataat catccacacc
     6721 cgcaaccggt caagacggtc atttccagac gcaatcgcgc aggcatcaca tttattctgg
     6781 tcttaatggc agttgccaca atgcaatctt gggcacaggc aaatatggtg aacctcatcc
     6841 caaaatacat caaagatttg gggcagggtg ccacg
//
LOCUS       DAOQJE010000058         6861 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_6150_length_6861_cov_6.413606, whole genome shotgun sequence.
ACCESSION   DAOQJE010000058 DAOQJE010000000
VERSION     DAOQJE010000058.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 6861)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6861)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6861
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6150_length_6861_cov_6.413606"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1477
                     /locus_tag="PLT08_02550"
     CDS             <1..>1477
                     /locus_tag="PLT08_02550"
                     /inference="COORDINATES: protein motif:HMM:NF019344.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNE03372.1"
                     /translation="EVQRAKGDVILMIDELHTVVGAGAAQGAMDASNMLKPALARGEL
                     QTIGATTLDEYHKHIEKDAALERRFAPIYVEEPSVEDTIKMLHGLRDKYEAHHKVRFS
                     DEALSAAAHLAVRYVTDRHLPDKAIDLMDEAAAKLRVALYSMPPDLKALKTEIDKLAA
                     EEEQAGLERDYERAAQKKAERLRLDQEYSGKRDQWEAEHHLDEVVDVNDIAAVVHQWT
                     GIPVTQMLETESEKLLHMEARLHERIIGQEEAIHAVSDAIRRARSGLKDPARPIGSFI
                     FIGPSGVGKTELAKALAWFMFDDEDALVRVDMSEYREQHTVSRLFGAPPGYVGYEEGG
                     QLTEAVRRRPYRVVLFDEIEKAHPEVWNALLQILDDGRLTDGQGNVVDFRNTVLIMTS
                     NLGTEYVRKGGTLGFLPGKADSSDREAHDKIDKALKAAFRPEFLNRIDEIIMFSPLTV
                     EEMEHIVILQMKEVQDRLNDHNITVQLTDAARSWLAKEGYDP"
     assembly_gap    1478..1577
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1578..2354)
                     /locus_tag="PLT08_02555"
     CDS             complement(<1578..2354)
                     /locus_tag="PLT08_02555"
                     /inference="COORDINATES: protein motif:HMM:TIGR03083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maleylpyruvate isomerase family
                     mycothiol-dependent enzyme"
                     /protein_id="HNE03373.1"
                     /translation="MTAILSTSNIARAEAIPMTHADEAHRLMTEALERLLSLLESLHA
                     DDWSKPTACTAWNVHDMVAHQAGGYLSGISLREMVRQYSQVPKKGQLPEDAINELQVG
                     ERKNKSHSELIAELKQVGAIAIHNWAYGFNAIKWIAMPHPVGGFMSIRHLMWVIHSRD
                     TWMHRLDICRATNRPFEQTREHDGRIVELVVLDTAKKIKKRLNGRAINLLLTGVAGGT
                     WQIGNGSPVAELEMDALDFNIFVSGRYSFEEGMQRAKISGD"
     gene            complement(2351..2986)
                     /locus_tag="PLT08_02560"
     CDS             complement(2351..2986)
                     /locus_tag="PLT08_02560"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HNE03374.1"
                     /translation="MKKTQPTSPRVQRNREVATSAILDTARAIMREEGVAALSMHELA
                     RRMGMRAPSLYNYFNSKMEIYDALFCLGFNLYNTQLQEALKESKTPHDEIRIAIECYM
                     SFALENPELYQLCFERPVPGFVPSKESLELSFGILNNTYTRIEKFKKQFDTKLTAQQI
                     VDMVIAVAHGLTALHLANEPQLPIGQGRFGSLIPTFVRFFETSMPTKGETK"
     gene            3132..>3973
                     /locus_tag="PLT08_02565"
     CDS             3132..>3973
                     /locus_tag="PLT08_02565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE03375.1"
                     /translation="MDNLENKSSGGMTSIIVGIVVLLCCLCLLAAGIGGYAYYTFMQS
                     GSTGPIEVFGDPISPDNNPTQPTEPTELIRPSVDSISSETVDTLENAIVPENDPRVLA
                     CRLDGKCGVPEVMATSASPRSVGEQQNFWVHDLDTNENNEVQATLRYITPHVYFWVQD
                     GLDIDEGEMKKLVDTFENQMYPTNREFFGSEWSPGIDGDEHIYILYSRGLGFSVAGYF
                     SSSDSTHPMIQEYSNAHEMFLFNADNTPLGGDYAYAVLAHEFQHMIHWNNDLNETSWL
                     NEGSS"
     assembly_gap    3974..4073
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <4074..4829
                     /locus_tag="PLT08_02570"
     CDS             <4074..4829
                     /locus_tag="PLT08_02570"
                     /inference="COORDINATES: protein motif:HMM:NF017368.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="immune inhibitor A"
                     /protein_id="HNE03376.1"
                     /translation="VHQYGVDYIAIECAGDFTINFTGSTVTGLLPADPYSGDYAFWSN
                     KGDESNMTLTHEFDLTNASGPVDLSFRTWFDIEEDWDYVYVEVSEDGETWQILTTPSG
                     RGTDPSGNSYGWGYTGVSGDWIEEKVDLSDYAGKKVFVRFEYITDAAVNGEGFMVDDV
                     KVDAAGYSSDFEADDGGWVAEGFVRVQNVLPQTFKLALIKSGDDNVTMIPVNADQTAE
                     IPVSLKSGETAYLVVTGTTRFTRELATYQIEIR"
     gene            4955..>6861
                     /locus_tag="PLT08_02575"
     CDS             4955..>6861
                     /locus_tag="PLT08_02575"
                     /inference="COORDINATES: protein motif:HMM:NF012742.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HNE03377.1"
                     /translation="MMTQSGNPSGKRKTIAVLGAQLSRVWGSEFMAGVLDAAHAVDFN
                     VVSFVGGKPSALQSHDGHSYGLYDLINPEQIDGVLLSADIAHGLSRDALQKFVEFLAP
                     LPVASFAVPSKGVPAVIADNDSGMRAIVRHLIEDHNYQRIAFVRGPVGQVEADQRYHA
                     FLDELKAHNIRFDQHLELEGDYSTESGRAAVRTLLDERGMAVQAIVASNDRMAFGVLE
                     ALQQRGITVPDKVAVTGFDDVSESQSMGVPLTTVRQSFYDAGAKTFELLLKRINGEEV
                     DDINLLPSSIVVRWSCGCLPESIHRAVVLPREVAHTGRLENKRVKAINALLAAAGVPE
                     TTPDRDVYVENFGVAWDTFLYSLRETDKSDAFLRSIQGMVEILQKHGYDFYTWQNVIS
                     TFRKYALGGISSTTTTLRAENLFQQARMLAGELSQRAQAYNRLQYVKQEELLNNFSFS
                     MASAVSFDDIGDAIARHFPILGIERWYVMYYGETDDAPGSISSPPPQSYRLLMQYDDK
                     RFTMPDDRSRLATGRLVPRGKTPEKRRYDAVVMPLSLATNRFGFMWAEMGPDDWDIYV
                     RVRNLLSSALLRTMLVNQREQAKREVDQLYAAEQARRHGAETLARSMRQLSSLKNVEQ
                     LPEQILEQLSQA"
BASE COUNT         1616 a         1536 c         1753 g         1756 t
ORIGIN      
        1 aggaagtcca acgcgcgaag ggtgatgtca tcctgatgat cgatgagttg cataccgttg
       61 tcggtgcagg cgcggctcaa ggcgcgatgg atgcgagtaa catgctcaag cccgctttag
      121 cgcgcggtga gttgcagacc attggcgcaa ccacgttgga tgaatatcac aaacacattg
      181 agaaagatgc tgcgctggaa cgacgtttcg caccgatcta tgttgaagag ccgagtgtgg
      241 aagatacgat caagatgctg cacggcttgc gcgacaaata cgaagctcat cacaaagttc
      301 gcttctcgga tgaagcactt tctgctgcgg ctcacttggc agttcgctat gtgaccgacc
      361 gtcacctgcc tgataaagcc attgacttga tggatgaagc tgctgcgaaa ctgcgcgtgg
      421 cgttgtactc catgccgcct gatttgaagg cattgaagac tgaaattgac aaactggctg
      481 ccgaagaaga acaagctggt ttggaacgtg attacgaacg cgccgctcaa aagaaggctg
      541 aacgcttacg ccttgaccaa gaatacagcg gcaagcgcga tcaatgggaa gccgaacatc
      601 accttgatga agtggtggat gtaaatgaca ttgctgcggt ggttcatcag tggacgggca
      661 ttcctgtcac gcagatgctt gaaaccgaat cagaaaaact cttgcatatg gaagcgcgtt
      721 tgcatgagcg catcattgga caggaagaag ccattcatgc tgtgtctgat gccattcgcc
      781 gcgcgcgttc tgggttgaaa gacccggctc gcccgattgg ttcgtttatc tttatcggac
      841 cttccggtgt ggggaagacc gaacttgcca aagctctcgc ttggttcatg ttcgatgatg
      901 aagatgcgct cgtgcgtgtg gacatgtctg aatatcgtga gcagcatacc gtctcacgtc
      961 tcttcggcgc gcctcccgga tatgttggct atgaagaggg cggtcaactc acggaagctg
     1021 tccgccgtag accgtaccgc gtagtgctat tcgatgaaat cgaaaaagct catcccgaag
     1081 tgtggaatgc cttgctccaa attttggatg atggacgcct gaccgacggt caaggcaacg
     1141 tagtggattt ccgtaacacg gttctcatca tgacctcgaa cctgggtact gaatatgtga
     1201 gaaaaggcgg caccttgggc tttttaccgg gcaaagccga tagctctgac cgcgaagcgc
     1261 atgacaagat cgacaaggcg cttaaggctg ccttccgccc tgagttcctc aaccgcatcg
     1321 atgagattat tatgttctca ccgcttactg tggaagagat ggagcacatc gtcatcttac
     1381 agatgaagga agtacaagat cgcctcaacg accacaacat cacggttcaa ctgaccgatg
     1441 cggctcgttc gtggctggcg aaggaaggct acgacccnnn nnnnnnnnnn nnnnnnnnnn
     1501 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1561 nnnnnnnnnn nnnnnnngtc gcctgagatt ttcgcgcgtt gcatgccttc ttcaaacgag
     1621 taccgacctg agacgaatat gttgaaatcc agtgcgtcca tttcgagctc tgcaacggga
     1681 gaaccatttc caatttgcca tgttccgcca gcgacgcctg tcaacagtaa attaatagca
     1741 cgaccattga gacgtttttt tatttttttc gctgtatcta aaacgacaag ctccacaata
     1801 cgcccatcat gttcacgcgt ttgctcaaaa ggacggttgg tagcgcggca gatatccaga
     1861 cgatgcatcc atgtatcacg gctgtgaatg acccacatta ggtgacgaat cgacatgaaa
     1921 ccgccaactg ggtgcggcat ggcgatccat ttgatagcat tgaaaccata tgcccagtta
     1981 tgaatggcaa tagcaccgac ttgttttagt tcagcgatta attcagaatg agatttattc
     2041 ttgcgttctc ccacctgtaa ctcattaatg gcatcttcgg gcaattgtcc tttcttggga
     2101 acttgggaat attgcctaac catctctcgt aaacttatgc cacttaagta tccacctgcc
     2161 tggtgtgcta ccatgtcgtg aacattccat gctgtgcagg ctgtaggttt agaccaatcg
     2221 tcagcgtgga gagactcgag caggctcaaa agccgctcca aggcttcggt catgagccga
     2281 tgggcttcat ccgcatgggt catggggatg gcttcagcgc gggcgatgtt tgatgtcgaa
     2341 aggatggcgg tcatttggtt tctcctttgg tgggcataga cgtttcaaag aaacgtacga
     2401 aagtagggat aagagaaccg aagcgtcctt gcccaatagg aagttgcggt tcgttggcaa
     2461 gatgcaaagc tgtgagaccg tgagcgactg caatgaccat atccacgatc tgttgagcgg
     2521 tgagttttgt atcgaattgt ttcttgaatt tttctatgcg tgtataggtg ttgttaagaa
     2581 tgccaaaact gagttcgagg ctttctttgg agggtacaaa accgggaacc gggcgctcaa
     2641 aacatagttg gtacagctca gggttttcta aggcgaaaga catgtaacat tcgatggcaa
     2701 tccgaatttc gtcatgtgga gtctttgatt ctttgagcgc ttcttgaagt tgagtgttat
     2761 acaggttaaa ccccaaacag aagagggcat catagatttc cattttgctg ttgaagtagt
     2821 tgtagagtga cggagcgcgc atgcccatac gacgcgccaa ctcatgcatg gagagggcag
     2881 caaccccttc ttcgcgcatg atagcgcggg ccgtatccaa aatagcagag gttgcgactt
     2941 cacggttccg ttgaacacgg ggggaggtgg gctgtgtttt cttcattttt ctaatattgt
     3001 tagttttttc taattatatt agaaaaattg cctttgtcaa gagattggct caccattacc
     3061 cctaaagtcc taatccgttt tttagcggat ggcggtatca tcggacaacc ttttaggaga
     3121 gaggaacatt catggataat cttgaaaata aatcatctgg cggcatgaca agcatcatcg
     3181 taggaatcgt tgtgctgtta tgctgtctct gtcttttggc ggcagggatt ggcggatatg
     3241 cctattacac atttatgcag tctggcagca ctggaccaat tgaagttttt ggcgacccca
     3301 tttccccaga taacaatccg actcagccaa cggaaccgac tgaactgatt cgcccgtcgg
     3361 tggattcgat ctcatctgaa actgtcgata cattggaaaa tgccattgtc cctgagaatg
     3421 acccgcgtgt cttggcgtgt cgtttagatg ggaaatgtgg cgtccctgaa gttatggcaa
     3481 cttcggcatc tccgcgttcg gtgggggaac agcaaaactt ctgggtacat gacctcgata
     3541 cgaatgagaa taacgaggtt caagcaacct tgcgttacat cacgccgcat gtctatttct
     3601 gggtgcaaga tggtctggat attgacgaag gcgaaatgaa aaagttggtg gataccttcg
     3661 aaaaccaaat gtaccccacc aaccgtgaat tcttcggcag tgaatggtcg ccgggtattg
     3721 acggcgatga gcacatttac atcctgtact ctcgtgggct cggtttctcg gttgctggtt
     3781 atttctcatc ttccgattcc acacacccta tgatccagga atattcgaat gcacatgaga
     3841 tgttcctgtt caatgcagat aatactccgc ttggcggcga ttatgcttat gcggttttgg
     3901 cgcatgagtt ccagcacatg atccactgga acaatgactt gaacgaaacc tcctggttga
     3961 acgaaggttc ttcnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4021 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnngttcacc
     4081 aatacggtgt ggactatatc gccatcgaat gtgctggcga ctttaccatc aacttcaccg
     4141 gctctaccgt gaccgggctt ttgccagctg acccctattc tggtgactac gccttttggt
     4201 cgaacaaggg cgatgagtcc aacatgacat tgacgcatga attcgatctc accaatgcca
     4261 gcggaccggt tgatttgtcc ttccgcactt ggtttgatat cgaagaggat tgggactatg
     4321 tgtatgtgga agtctctgaa gacggcgaga cttggcaaat tctcactaca ccttctggta
     4381 gaggtacaga cccaagcggc aattcgtatg gctgggggta taccggtgtt tctggcgatt
     4441 ggatcgaaga gaaggttgat ctttctgatt acgctggtaa gaaagtcttt gtccgctttg
     4501 agtacattac cgatgccgcg gtcaatggag aaggcttcat ggtagatgat gtcaaagtcg
     4561 atgcagcagg ctactcgtct gattttgagg cggatgatgg cggttgggtg gcggagggat
     4621 tcgtgcgcgt ccagaatgtc ctcccgcaaa ctttcaagct ggcgctcatc aaatctggcg
     4681 acgacaatgt gactatgatc ccggtcaacg cagaccagac agcagagatc ccggtttcgt
     4741 tgaaatctgg tgaaacggct tacttggttg tgaccggaac gacccgcttc acacgtgagc
     4801 tggcgaccta tcagatcgag atccgctaag cttttctgaa atttctacat tgacagccgc
     4861 ttttatggcg gctgtcaatt tttttaaggc ggagattgtc ttgcaaaatt gcaatttttg
     4921 gctcaggcat cctgccgtat aattttgtaa ctccatgatg acccaaagcg gaaatccttc
     4981 tggcaagcga aagacgatcg ccgtcctcgg tgcacaactc agccgagtgt ggggcagtga
     5041 gtttatggcg ggcgtattgg acgccgctca tgcagtggat ttcaatgtcg tttcttttgt
     5101 aggcgggaaa ccttcggcgc ttcaatctca tgatggacat tcttacggtt tatacgacct
     5161 cattaatccc gaacagatag acggtgtttt gctctctgca gatattgccc acgggctttc
     5221 acgagatgca cttcaaaagt ttgttgaatt tctcgcgcct ttgccggttg cttcattcgc
     5281 tgtgccaagc aagggcgttc ctgctgtgat cgcggataat gacagcggta tgcgtgcgat
     5341 cgtacggcac ttgatcgaag accataatta tcaacgcatc gcctttgtcc gtggacccgt
     5401 ggggcaggta gaggcagatc aacgttatca tgcctttctg gatgagttga aagctcataa
     5461 tattcgtttc gaccaacatt tggaattaga aggcgattac tccaccgaaa gtggccgtgc
     5521 cgcagtgcgc accttgcttg atgaacgtgg catggcagtg caagccattg tcgcttcgaa
     5581 tgaccgaatg gcattcggcg tgttggaagc gttacaacag cgcggtatta cagtgcctga
     5641 taaggttgcg gttacgggtt tcgatgatgt cagtgagtca caatctatgg gggtgccttt
     5701 aaccaccgtc cgccaatcgt tttatgatgc gggtgcaaag acatttgaac ttctcctgaa
     5761 acgcatcaat ggcgaagaag tggatgatat taatttgttg ccatccagca ttgtagtgcg
     5821 ttggtcttgt ggttgtttac cagagagtat tcatcgtgca gttgtgttgc cacgtgaagt
     5881 agcgcacaca ggccggcttg aaaacaaacg tgtgaaagcc atcaatgctt tgcttgcagc
     5941 agcaggcgtg ccggaaacta cacctgatcg cgatgtctat gttgaaaatt ttggtgtggc
     6001 atgggacacc ttcctttaca gtttgcgcga aaccgataag agtgatgcgt tcttacggtc
     6061 aattcaaggc atggttgaaa tacttcagaa gcatggttat gacttctata cctggcaaaa
     6121 cgtgatctca actttccgta aatatgcgtt aggtggcatt tcaagcacaa ccactacatt
     6181 gcgtgctgaa aatcttttcc agcaggcacg tatgttggcg ggcgagttgt cgcagcgggc
     6241 tcaggcatat aaccggcttc aatatgtaaa gcaggaagaa ctgcttaata actttagttt
     6301 ttccatggca tcggctgttt cgttcgatga tatcggcgat gcgattgcta gacatttccc
     6361 cattctcggt attgagcgct ggtatgtgat gtattatggc gagacggacg atgcgccggg
     6421 ttcgatttct tcaccgccgc cgcaaagtta tcgtttgctc atgcaatatg acgataaacg
     6481 cttcaccatg ccggatgatc ggtctcgact tgcaacaggt cgccttgtgc cacgcggaaa
     6541 gactcctgaa aaacgtcgtt acgatgccgt agtcatgccg ctctctttgg caacaaatcg
     6601 cttcgggttt atgtgggcag aaatgggacc cgatgattgg gatatttacg tccgcgtgcg
     6661 aaacttgctt tcaagcgcgc tcttgcgtac gatgttggtt aatcaacgtg agcaagccaa
     6721 acgtgaagtg gatcaacttt atgcggcgga acaggcacgg cgtcatggtg ccgagacgtt
     6781 ggcacgttcg atgcgtcagt tgtcttcact aaaaaatgtt gaacagttac cagaacagat
     6841 tctagaacaa ttaagccaag c
//
LOCUS       DAOQJE010000059         6821 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_6200_length_6821_cov_6.153562, whole genome shotgun sequence.
ACCESSION   DAOQJE010000059 DAOQJE010000000
VERSION     DAOQJE010000059.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 6821)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6821)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6821
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6200_length_6821_cov_6.153562"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1243)
                     /locus_tag="PLT08_02580"
     CDS             complement(<1..1243)
                     /locus_tag="PLT08_02580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE03378.1"
                     /translation="MKQLAWAGFIGLGVQIFLMSLVFGLLWYLTSDDRFKGWYASLLA
                     ASLFGIPALLLRALGPNNDQLGSILQFVLAVIVALAVIYIRKDKLVWDFSALPFGFLV
                     AALGFAPLAVYGSFGSPTDVVLSLFAGLAFGLLAAVLMSDAENIFLNGIGAGGLLALL
                     ASSFGYDGSQLILVVLVPAFGCAIAALYPSRAAATVAAGLLAFAGLAFFDPTELTIVL
                     GDIGGLAVKAVGIALLIGWGVSVVALILRQVMGSGSTSGVKRAAGWVGAVAGWVMVIA
                     AFFIWGNPGNYSDRLFVIFKDQADISDVASIKNRDERLTEAYMRLTDHANKTQADLRE
                     TLDTFGVAYTPYYLENAMEVQGGTLVRLFLMTRSEVDRVIPSPRLRAAPEDSPEPGFE
                     SAPPADYVGWNIQLIGADRVWE"
     assembly_gap    1333..1432
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1433..1884)
                     /locus_tag="PLT08_02585"
     CDS             complement(<1433..1884)
                     /locus_tag="PLT08_02585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE03379.1"
                     /translation="MIKPVRWNTFIRDFIRIQIGFLLYGLAITLMIRGNLGTSAWAVL
                     EVALADFLHITVGTMTVIMGFIVLILSLLMREQIGWGTLGNILSIGPWVDLCMALVPT
                     VNNNLAIQILMLLSAIIIMGLASAIYIGVDAGAGPRDSLMLAIRRKTGV"
     gene            complement(1947..>6821)
                     /locus_tag="PLT08_02590"
     CDS             complement(1947..>6821)
                     /locus_tag="PLT08_02590"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HNE03380.1"
                     /translation="QAHTAEDFYIAVGREINQLGGEVTLLLLDEAGTKLSIAYTSYSP
                     KLISRAEKLTGLSLKNYKLPIPPESAYEVALQGESAIFIDASDEIISNILPDALAFLS
                     TQIISLFNLRQGILAPLRVDKKVLGLLKVNGNFLNLDDLPAMESFAGHIAAGLHNVHL
                     MQKLQEELTAHKQTEQALQHNRNLLLALSQAAHAIQLVRMPDDIYKVVGEQIRALGFE
                     ATILKFGSDMNSLHYRYTTIPEKVLHTAEKLAGIKAEDFGWAISQDSVYARVLINRKA
                     EYISNGKALFIEALPKFLLPLAEKLVDLVSSHHGILAPLHVDDETFGIMLVFGSDQIS
                     EEDVPAVESFSAQVSVSLRNAYLTQRLQRELHERKQAEAAAQQAEKRFKALIENAPDG
                     ITLVGIDGKATYASPSAKEMFGYVGDEYINENPMDSIHPDDIGAVLATMNNLVGDSSS
                     PRTVQYRYRHQDGSYHWVESIFSNQFAEPAVEAIVVNFRDITERKEMERALYESERYY
                     RALIENTADGILVIDGSGVVTYESPSVARLLGYGQDKLIGTSSFNLIHPDDLSEILKT
                     FVDGLNSPGFVHRGEYRLLNAENNWRYFEIVTHYLMDDPAIMGVIINGRDITERKQSE
                     SALKESERKFHGVIDKSADGIALSDEEGRILEFNEAFEQLTGKKRENVLGKFLWELAY
                     ELMPDSTKADDENFTLRNRIQQALKIGATSYFQFTQEVPFQRADGSVLIIQQRIFTIR
                     TDKGCRLGSISRDITEIKKVEEYLTQQNKQLNSLYQITSALSQINAVEDIYAIALDSL
                     CSNLDIKRVSILLFDRDGVMRFKAWRGLSDRYRQAAEGHSPWKPDAVDPQPVLVEDAF
                     TDAELASLYELFKSEGIGALGFIPLVHQNKLLGKFMIYFDAPHHFLETEVRLAQAIAS
                     HVAFAIHKSVLNEALRTSEEQYRILYEDNPSIYFTTDDMGTILSVNKTGVEQLGYAID
                     ELIGSPLLNIFYPDDREHVQQKMLVCINDPELTVQVEARKQRKDKSILWVREFARAIR
                     NTDGQLVILIVCNNVTEEVKAEEALVQSEAELRALFSSMKDSVIVIDRKGIYQSIAPT
                     NPETLYIPPAEVIGKGLSAFFPEEQVQEFLKAIEYVLATGKTVIIEYQIILADASPWF
                     ESRVSPLGPDTTIWVARDVSERKKIQSALIQSEQAYRSLFENMPIGLYRSSIHGELLD
                     ANPSLLRMFGYEDLETMKGKKAWEFYNDPALDDEFRQQISENGFLNRYESEFIRQDGT
                     RFWAEDYVRAVYDGKGIPLYYEGSLIDVTDRKAAEKRLKESEKQYRLLAERIADVVWV
                     LDLATLEFKYISSSVQNLLGHSAEELLNKHVSMVLTPSAMMAIESITPERIKRFLAGD
                     KTSVTELYQLDHVHKDGSIIVVEVSSTFVMNENATIEAIGVTRNITERKKAEDDLRLA
                     NSSLQIAHKELQQLFEYEQILARTDGLTRLYNRRYFFELAAREFNSAIRYQRPLTLII
                     FDVDGFKQANDTFGHDFGDRTLVEVSKTANLQVREVDILARYGGDEFTILLPETNVEQ
                     AFLIAERIRKAVADVYIDMGGFSTFVTISMGIAELSFEPQDQSIEDMIRRADQALYQA
                     KQQGRNKVVIYSDK"
BASE COUNT         1956 a         1645 c         1337 g         1783 t
ORIGIN      
        1 cttcccacac tcgatccgcg ccgataagtt gaatgttcca accaacataa tctgcgggcg
       61 gcgcagactc aaagcccggt tcaggagaat cttcgggagc ggcacgcagg cgcgggctag
      121 gaatgacgcg gtccacttcg gagcgggtca tcaagaacag gcgcacaagc gtcccacctt
      181 gcacttccat cgcattctct aaataatatg gcgtatatgc cacaccaaat gtatctagcg
      241 tttcacgcag gtctgcttgc gttttatttg catgatccgt aaggcgcata taagcttcgg
      301 tcaaacgctc atcgcggttt ttgatggatg ctacatccga aatatcggct tggtctttga
      361 agataacaaa gaggcggtca ctataattgc cggggtttcc ccagatgaaa aaagcggcga
      421 tgaccatgac ccaccctgcc acagcaccta cccatcccgc cgcccgcttc acacctgacg
      481 ttgagcctga tcccattacc tgacgcagaa tcaacgccac aacactgact ccccaaccga
      541 tcagcaaagc aatccccaca gctttcacgg caagcccgcc aatatcaccg agcacaatgg
      601 tgagttcagt tgggtcgaag aatgccaagc cagcaaatgc aagcaaccca gccgcaactg
      661 tagctgccgc ccgcgatgga tataaggctg cgatagcgca accaaaagca ggaactaaaa
      721 caaccaatat caattggctt ccatcatatc caaacgaaga agccagtaat gccaacaaac
      781 cacctgcgcc aatgccatta agaaagatat tttcagcatc tgacattaac acggcggcaa
      841 gcaagccaaa tgccaaaccc gcaaacagac taagcaccac atcggtgggc gacccgaacg
      901 agccatacac tgccagcgga gcaaacccca atgccgcaac caagaacccg aatggcaacg
      961 cactgaaatc ccaaactagt ttgtccttac gaatatagat caccgccaag gcaacgataa
     1021 cggcaaggac aaactgtaaa atagaaccaa gctggtcgtt gtttggtccg agtgcacgca
     1081 aaagcaaggc tggaattcca aacaaggaag cggctaacaa gcttgcatac catcctttga
     1141 aacggtcatc actggtaagg taccaaagca agccaaacac caggctcatc aggaagattt
     1201 gcacacccaa accaataaac cctgcccatg ccaattgctt catactcgag gaggcaattg
     1261 cgctctgttc ggtagaccaa acaataaaat tatagataaa caaacaaaag ggcattggca
     1321 aaagcacaat gannnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1381 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnacgcccgt
     1441 cttgcgccga atcgcaagca tcaagctgtc tcgtgggcca gcgcctgcgt caactccaat
     1501 atagatggcg ctggcaagtc ccataatgat gatggcgctt aacaacatga gtatctgtat
     1561 agcaagattg ttattcacag taggaaccaa cgccatgcat aagtccaccc agggaccaat
     1621 ggaaagaata ttccctaacg tcccccaccc tatctgttcc cgcattaaca aggagagtat
     1681 caaaacgatg aagcccataa taacagtcat ggtacccacc gtaatgtgta aaaagtctgc
     1741 caatgccact tccaatactg cccaagcact tgttccgagg tttccgcgaa tcattaaggt
     1801 gatcgccaag ccatacaaaa gaaagccgat ctgaatgcga ataaaatcgc ggataaatgt
     1861 attccaacga actggtttaa tcatgatgct cctttgaaag catcatgata ccatgccgct
     1921 ataaaccttg attttttgtg atgcgattat ttatctgagt aaattacgac tttattccta
     1981 ccctgttgtt ttgcctgata caaagcctgg tctgcccggc ggatcatatc ttcgatcgac
     2041 tgatcttgag gttcaaaaga taattctgcg attcccatgc tgatagtaac aaacgtggag
     2101 aaaccaccca tgtcaatata gacatctgca actgctttac gaatacgttc tgcaatgaga
     2161 aaagcctgct ctacatttgt ctctggcaaa agaatcgtaa attcatcacc accgtaacgt
     2221 gccaaaatat ccacttcacg tacttgtaaa tttgctgttt tggaaacctc aaccaatgtg
     2281 cgatccccaa aatcatgtcc gaatgtatcg tttgcctgtt tgaaaccgtc tacgtcaaaa
     2341 ataatcaatg tcaatggtct ttgatagcga attgcggagt taaactcgcg tgcggcaagc
     2401 tcaaagaaat agcggcggtt gtataaacgc gtcaaaccgt cggttcgagc aagaatctgt
     2461 tcatactcga acaattgctg aagttcctta tgggcaattt gaagagaact attggcaagg
     2521 cgcaggtcat cttctgcttt ctttcgctcg gtaatattac gagtcacacc aatcgcttct
     2581 attgtagcgt tttcattcat aacaaatgtg cttgaaactt caaccacaat aattgaacca
     2641 tctttatgaa catggtccaa ctgataaagt tctgttacag atgttttatc tccagcgagg
     2701 aagcgcttta tgcgttcagg ggtgatgctc tcaatagcca tcatagcaga aggagttaac
     2761 accatgctga catgtttatt cagcaattcc tcagcagaat gccctaaaag gttttggaca
     2821 gacgagctaa tatatttgaa ttcaagagtt gccagatcca aaacccaaac cacatcggca
     2881 attcgttcag ccaataggcg gtattgtttc tcgctttcct taagtcgttt ttctgctgct
     2941 tttcgatccg ttacatcgat cagactgcct tcgtaataaa gtggtatacc tttcccatca
     3001 tacacagcac ggacataatc ttctgcccag aatctggtcc catcttgacg gataaattca
     3061 gactcatatc gattcaaaaa accattttca gaaatctgct gtcggaactc gtcatctaaa
     3121 gcagggtcat tataaaactc ccatgccttc ttccccttca ttgtttctaa atcttcatat
     3181 ccaaacattc gcaacaaaga tgggttggca tccagcaact cgccatggat agaagaacga
     3241 tatagtccga ttggcatgtt ctcaaacaaa gaacgatagg cttgttcaga ttggatcaat
     3301 gctgattgga tcttcttacg ttcagaaaca tctcttgcca cccagatggt tgtatcgggt
     3361 cccaacggcg agactctcga ctcgaaccat ggagaagcat ctgccaaaat gatctgatat
     3421 tcgataatga ctgtcttacc ggttgctagc acatattcaa ttgccttgag aaattcctga
     3481 acttgctctt cagggaagaa cgcggatagt cccttcccga tcacctcggc aggagggata
     3541 taaagtgttt ccgggtttgt aggagctata ctttgataga tcccttttcg gtcaatcaca
     3601 ataacagaat ctttcattga agagaataat gcccgtagct cagcttcaga ctgaacaagg
     3661 gcttcttcag ctttgacttc ttctgtaaca ttgttacaaa cgatcagaat caccaattgt
     3721 ccatctgtat tcctgatagc acgcgcaaac tctctcaccc atagaatgct tttatctttc
     3781 ctttgttttc gcgcctcaac ctgaacagta agttcagggt cgttgataca caccaacatc
     3841 ttttgctgaa catgttcgcg gtcatctggg taaaagatgt tcaatagtgg gctgccgatc
     3901 agttcatcaa tggcataccc taattgttcc acaccagttt tattgacaga aaggatagtg
     3961 cccatatcat ctgtagtgaa atatatggat gggttatctt catacagaat acgatattgc
     4021 tcttcgctcg tacgtaaagc ctcatttaac acgctcttat gaatagcaaa cgctacatgc
     4081 gaagcgattg cctgagctaa gcgaacctct gtttcaagaa aatgatgtgg ggcatcaaaa
     4141 tagatcataa acttgccaag cagtttgttc tgatgaacaa gcggaataaa accgagggcg
     4201 ccaatcccct cgctcttaaa gagctcatac aaagaagcga gttcagcatc agtgaatgca
     4261 tcttctacca aaaccggttg cggatccact gcatctggtt tccaaggaga gtgtccttct
     4321 gctgcttggc gatatctatc tgacaatccg cgccaggctt taaaacgcat caccccatct
     4381 ctatcaaata atagaatgga tacccgctta atatccaaat tactacataa actatcgagg
     4441 gcaatggcat agatatcttc aactgcgtta atttgactta aagcagaagt aatttgatac
     4501 aaactattca gttgtttgtt ttgctgcgtg agatattctt ctactttttt gatctcggta
     4561 atatcccgcg agatcgaacc aagcctgcac cccttatctg tacggatcgt aaatattctt
     4621 tgttggatga ttaatacact accgtccgcc cgctgaaatg ggacttcctg cgtaaattgg
     4681 aaataagaag tagcgccaat tttgagagct tgttgaatcc gatttcgaag agtaaagttt
     4741 tcatcatctg cttttgtact gtctggcatg agttcataag caagctccca caggaattta
     4801 cctaagacat tctctcgctt tttccctgta agttgttcaa atgcttcatt gaattcaaga
     4861 atgcgtccct cttcatccga tagtgcaatg ccatctgcag atttatcaat tacgccatga
     4921 aattttcttt cactttcttt caaggcactc tcagactgtt tccgctctgt aatatcccgt
     4981 ccattgataa tgacgcccat aatcgcaggg tcatccatca agtagtgagt gacaatttca
     5041 aaatagcgcc aattgttttc tgcattcaat aagcgatatt cgccacgatg tacaaatcct
     5101 ggagaattta agccatcgac gaacgtctta agaatctcag ataaatcatc aggatggatc
     5161 aagttaaatg aggatgtacc gatcaattta tcttgcccat accccaacaa acgtgcaacg
     5221 gatgggcttt catacgtcac aaccccgctc ccatcaatca ccagaatccc atccgcagta
     5281 ttctctatca gagcgcgata gtagcgttca ctctcatata gagctctttc catctcctta
     5341 cgttcggtaa tatctctgaa gttaacaact attgcctcta ctgcaggttc tgcaaactga
     5401 ttgctaaata tactttccac ccaatgataa gaaccatctt ggtgtcggta acgatactga
     5461 accgtacggg gagaagagga atctcctacc agattgttca tcgttgcaag aacggcgcct
     5521 atatcatctg ggtggataga atccataggg ttttcattga tatattcatc cccgacatag
     5581 ccaaacatct ctttggcaga tgggctggca tacgtagcct taccatctat tcctacaagg
     5641 gtaataccgt ccggcgcatt ttcaatcaaa gctttgaagc gcttctctgc ttgttgagcg
     5701 gcagcttctg cctgttttcg ttcatgcaat tctctttgca acctttgagt taagtaggca
     5761 tttcgcaaac tgaccgaaac ttgggcggag aaagactcga cagctggaac atcctcttcg
     5821 cttatctgat cacttccaaa caccagcata atgccaaagg tctcatcatc aacgtgtaat
     5881 ggtgccagaa tgccatgatg tgaacttaca aggtcaacta gtttttcagc tagcggaagt
     5941 aaaaatttgg gaagcgcctc aataaaaaga gcttttccgt ttgaaatgta ttccgctttt
     6001 ctattgatca aaacacgagc ataaacacta tcttgcgaaa tagcccagcc aaaatcttct
     6061 gctttaatac ctgccaattt ttcagcagta tgaagtactt tttctgggat ggttgtatag
     6121 cgataatgaa gactattcat atcgcttcca aacttaagga tagttgcctc aaagcccaat
     6181 gcgcggatct gctctccaac cactttatag atatcatctg gcatgcgcac cagttgaatg
     6241 gcatgggctg cctggctcaa ggcaagtaaa aggttacggt tatgctgaag agcctgctcc
     6301 gtctgtttgt gagccgttaa ttcctcttgt agtttttgca tcaaatggac gttatgaagt
     6361 ccagccgcaa tgtgccctgc gaatgactcc atcgcaggga gatcatccag attgagaaaa
     6421 tttccgttga ccttgagcag tcccaaaact tttttatcta cacgtagagg cgcaagaata
     6481 ccctgacgca aattaaaaag agagataatt tgagtagaca aaaaggcaag cgcatctggc
     6541 agaatatttg aaataatctc atcacttgca tcgataaaaa tcgcagattc tccctgcaaa
     6601 gcaacttcat atgcgctttc aggaggaatt ggaagtttat aattttttag ggatagcccg
     6661 gttagctttt ctgcccggct gattaatttt ggggaatagg atgtataagc aatagaaagt
     6721 tttgtccctg cttcatcaag cagcaacaaa gtgacttccc caccaagttg gttgatctct
     6781 ctgcctaccg caatataaaa atcttctgct gtatgtgcct g
//
LOCUS       DAOQJE010000060         6816 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_6206_length_6816_cov_6.122023, whole genome shotgun sequence.
ACCESSION   DAOQJE010000060 DAOQJE010000000
VERSION     DAOQJE010000060.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 6816)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6816)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6816
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6206_length_6816_cov_6.122023"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..692)
                     /locus_tag="PLT08_02595"
     CDS             complement(<1..692)
                     /locus_tag="PLT08_02595"
                     /inference="COORDINATES: protein motif:HMM:NF012509.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase family protein"
                     /protein_id="HNE03381.1"
                     /translation="MATTIFSPYRICPIGAHIDHQGGAVLGRTIQLGTTLTYEPNTSK
                     EIHLISARYGETAFTIGDLDLSHWARYAQAAARVLKPKIGMHAQVHGSLIGAGLSSSA
                     SVGLAYLKALADVNHIGLTSEQLIQLDFELEHDQLGLQNGLLDPMSIVHGKKNALLFM
                     DVVTGSVSPVFDSTENASAWIVAYSGISRELTKSGFNVRVAECHEAATLMKTSAQKLG
                     DVPRDVFELKKKS"
     gene            complement(743..964)
                     /locus_tag="PLT08_02600"
     CDS             complement(743..964)
                     /locus_tag="PLT08_02600"
                     /inference="COORDINATES: protein motif:HMM:NF038399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Os1348 family NHLP clan protein"
                     /protein_id="HNE03382.1"
                     /translation="MSAQAIESILTRAMNDVTFADMFFANPDLTLAGFDLTAEEIVKL
                     KSMSRSELENATPETRLDIIKKLQSQDSS"
     gene            complement(1022..1663)
                     /locus_tag="PLT08_02605"
     CDS             complement(1022..1663)
                     /locus_tag="PLT08_02605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE03383.1"
                     /translation="MKQYRMLFAFAALMLVVSLACGGSSVPATENPTLPPVPTNPPAQ
                     QEQPQGQDQSQGQTTTDSGLVTFVDENGLAAFDLPGDWVYSHSTGDNYYVDRFTTPDG
                     FSGLVESLVYNDGTSFVKSQKGQFALDLINTFYSATGEVGDIKVTSDQIMPDGSERLE
                     WTSKSGGYSGMTFFETRGDDNSTFMMLSAWWDNNADQATLDIINNAIASYHIP"
     gene            complement(1721..4567)
                     /locus_tag="PLT08_02610"
     CDS             complement(1721..4567)
                     /locus_tag="PLT08_02610"
                     /inference="COORDINATES: protein motif:HMM:NF013124.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BTAD domain-containing putative transcriptional
                     regulator"
                     /protein_id="HNE03384.1"
                     /translation="MSQSPTIKIYLLGSVHLERGKETLEFARQKTLLLLAYLALHPQE
                     HPRERIASIFWKDATTDEAARLSLRVTINDLRKTLGEDSLIGGRDSLQLNPDLNLWID
                     VREFQRLTDSRATPEDWQSALSLYTGDLLPQVYEDWVLEWRAEFEQACLETRLRLAQH
                     QRGQGQYVAAIETARLALEIEPVRESTHQLIILCYESLGDREAALLQYESCSKALREI
                     GVEPSPELQGIYRRLQKTPASLHITGNLPRPLTSFIGREEELNEVETLLAETRFLTLL
                     GAGGSGKTRLAIQAADESAHNYPDGVWWVDLAPLLKGDQLPQAIAKSLGVKEKLGADM
                     LDHIASFIGERKMLIILDNCEHLVSATAHSIEAVSAKCQAVTFLATSREPLGVASEIG
                     WQTPAFPLPEPGASHLKTLAKNESVRLFVERGRAVNGSFQLNGQNVMRVAEICRRLEG
                     MPLAIELAAAQVNNLTLDGILSRLEKSLNLTSTDAAHARHAALHSAIEWSYNLLTPAE
                     QMLFRRLAIFSGGWNLASAAIVAAGYSDPEAPLSNEASEGTIAPLPINGETVTRQILD
                     ALVKKALAQFRRAEGSLRYDTLDTLREFAREKMNEVGETQIMQERHALTFFNFLKKAW
                     DDVYSERESSIFNQIDLEYANLRTAWAWQLQSPKWVVDAKAINSQNRFWYVRGHYAEG
                     RETLEALLKHPDVHLNTLIHATLLNNLGLIEWKSGNRIAAKRIFEEALVEFRTLQLDF
                     AAAMIIANLAGLYIDDDDMEKGLPLAHEAVVVARETGNKSALNISLNNLAIALTITRD
                     FKGAREAFYEGLALSKEQGDIRGEGIIYNGLADIEAEEGNFAEARRLFTQSFMTRSSL
                     GDRRGSIIPALNLIRLMCMDEDWENAACLLGFVEETLQQLGIQLASDARAGREEANRS
                     VRLALDEETVTRALQFGKTLTLDSANRWLAEKSL"
     gene            4833..>6816
                     /locus_tag="PLT08_02615"
     CDS             4833..>6816
                     /locus_tag="PLT08_02615"
                     /inference="COORDINATES: protein motif:HMM:NF024589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNE03385.1"
                     /translation="MAFSLWADEDEASSLANLRRHLYLLKKSLPKALQPSLIISTQTV
                     LFQFPANTWVDVLAFEQEPTSLQEIEEHALFYTTDLAQGAETDEFIFSRREELRNRYA
                     QLLRMLAQTYFNYAEYETSMYWVRKLIAQDPWDEEALRLYMTSDFILGNRTTALSTYK
                     SFEKKLRLEMDIQPMPETMALYRDILHNHLLRPVSSTKTEGETFISRLSEIQTLGNAI
                     QAHGQYGNFVIINGTAGVGKTTLVREALRRFLNSSKAKLLWGDCQPAGEEQPYSIWQQ
                     IFNAAVPLLARQQDVAQEWLNHLLPLIPDLGLLRADLFSPTRPDATELRTAMRQTLFA
                     LSESRPLIIVIEDAHWMDVPSLEFLRELAESSLNIPVLFILTHRTDDAALPLFEIKRI
                     LRRKRNVVDISLQPFTTTETNQYLEKFLGTKTLPKELIDDLMQYSGGLPLLLHEVTQT
                     LNSSKRILSLREAFLFRLDRLSPAAREMVETAAVIGFSLTDIELRTALGWAAELYAST
                     MDALQAERFLLENPSNSADEYAFSHRLIHEIIVEQISVERISNLHIRVARALEQIHSE
                     DIGFAAEIALHYQQAGQLILAAGFWVRHASESTDLAAFDSGLAAINQAEAILTGASLD
                     AQEVHAKLALQRGVIVFYQGKSEEAFSLLEKAVEYSK"
BASE COUNT         1710 a         1587 c         1681 g         1838 t
ORIGIN      
        1 gattttttct tcaactcaaa gacatcacgc ggcacatcac ctagtttttg cgcgcttgtt
       61 ttcatcaacg tcgccgcttc atggcattcc gccacgcgga cgttgaatcc gcttttggta
      121 agttcgcgcg aaatgcctga gtacgccacg atccacgccg aagcattttc agtggagtca
      181 aagactgggg aaacagaacc cgtgaccacg tccatgaaaa gaagcgcgtt cttttttcca
      241 tgcacaatgc tcattgggtc gaggagtccg ttttgcagcc cgagttggtc atgctccaat
      301 tcaaaatcga gttggataag ctgctcgcta gtgagtccaa tatgatttac atctgcaagc
      361 gctttgagat acgccaatcc caccgaagca gatgaactca agcctgcgcc gatcaacgaa
      421 ccatggactt gggcatgcat gccaattttt ggcttcagaa cccgcgccgc cgcctgggca
      481 taacgtgccc aatgcgaaag atcgagatcg ccgatggtaa aagctgtttc gccgtagcgc
      541 gcgctgataa gatgaatctc tttggaagtg ttcggttcat aggtaagtgt ggtgccaagc
      601 tggatcgtgc gtcccaacac tgcgccgccc tgatggtcga tgtgcgcgcc gatgggacaa
      661 atgcgatacg gagaaaagat ggttgttgcc ataacgcggc gagtctaaca tgaaaaaaag
      721 agcatctttg cgagatgctc ttttagctcg aatcttgtga ctggagtttc ttaataatgt
      781 ccagccgtgt ttctggggtg gcattctcaa gttctgaacg cgacatgctc ttgagtttta
      841 cgatctcttc agcggttagg tcgaagcctg ctaaggttaa gtctgggttg gcaaagaaca
      901 tatctgcgaa ggtcacgtcg ttcatggcac gagtcaaaat gctttcgatg gcttgagcag
      961 acatggaatg tccttgaaac gagcaggcag gttgacctgc ctgctcgttt tgtgggttta
     1021 tttacggaat atgataggag gcaatggcgt tattgatgat atctaaagta gcttggtcag
     1081 cgttgttatc ccaccaagca gagagcatca tgaaggtgct gttatcgtcg ccacgggttt
     1141 cgaagaaggt cataccagag tagccgccgc ttttggaagt ccattcgaga cgttcggacc
     1201 catcgggcat gatctgatcg ctggtaactt tgatgtcacc gacttctccg gtggcgctat
     1261 aaaaagtgtt aatgagatcg agggcgaatt gtcccttttg gcttttgacg aaagaggttc
     1321 cgtcgttgta gacaaggctc tctactaagc cgctgaagcc gtcgggggtg gtgaagcggt
     1381 cgacatagta attgtcaccg gtggagtgac tgtagaccca atcgccggga aggtcgaagg
     1441 cggcgagacc attctcgtca acgaaggtga ctaagccact gtctgtggtg gtttgacctt
     1501 gtgattgatc ctgtccttgg ggctgttcct gctgagcagg cgggttggta gggacggggg
     1561 gcagggtggg gttttcagtg gcgggtactg acgagccacc acaggcgagg ctgacgacaa
     1621 gcatcagcgc agcaaaagca aataacatac ggtattgttt catgagaaat tctcctttgt
     1681 tgaatttcct ctatgataga aacgtaccgt tacataagcg ttacagggat ttttctgcca
     1741 gccagcggtt tgccgaatca agtgtcagtg ttttgccaaa ttgaagggca cgagtaacag
     1801 tttcctcatc cagcgcgagg cggacggaac gatttgcttc ttcacgccca gcacgtgcat
     1861 cagatgcaag ttgaatcccg agttgttgta gggtttcttc cacaaaccct agcaaacagg
     1921 cggcattttc ccaatcttca tccatgcaca ttaagcggat caagttcagt gcagggatga
     1981 tggaaccacg gcggtcgccg aggctggagc gcgtcataaa gctttgggtg aacaggcggc
     2041 gggcttctgc aaaattgcct tcttccgcct cgatatctgc caagccgttg tagataatac
     2101 cttcgccacg gatatcgcct tgttctttgc tcaaagcaag accttcatag aaagcttcgc
     2161 gagcgccttt gaaatcacga gtgatggtga gcgcaatggc aagattgttc aaactaatgt
     2221 tgagggcact tttatttccg gtttcacgtg ccacgaccac ggcttcgtgc gccaggggaa
     2281 gccccttttc catgtcatcg tcatcgatat aaagcccagc caggttcgca atgatcattg
     2341 cggcggcaaa atctaattgt agggttcgaa attctactaa tgcttcttcg aaaatccttt
     2401 ttgctgcaat acggtttccg cttttccatt cgattaaacc aaggttgtta agcaaagtgg
     2461 catggatgag tgtatttaaa tgtacatcag gatgtttgag caaggcttcg agagtttcac
     2521 gtccttcagc gtaatgtccg cgtacatacc aaaaacggtt ctggctatta attgccttgg
     2581 catctaccac ccattttggc gattgcagtt gccatgccca tgcggtgcgc agattggcat
     2641 attccaaatc tatttgattg aatatgcttg attcgcgttc tgaataaaca tcatcccaag
     2701 ctttttttag aaaattaaaa aatgtgagag catgccgttc ttgcatgatc tgggtttcac
     2761 caacttcatt cattttttcg cgtgcaaatt ctcgtaaggt gtctaacgtg tcgtatcgca
     2821 agctgccttc tgctcgacgg aattgtgcta atgctttttt tacaagtgca tccagaatct
     2881 gtcgtgtaac cgtttctcca ttgatgggca atggtgcgat tgttccttct gaagcttcat
     2941 tcgaaagagg cgcttcgggg tcagaatagc ctgccgctac aatggcggct gaggcgagat
     3001 tccatccgcc agaaaaaatt gccagccgac gaaaaagcat ttgttctgct ggggtaagca
     3061 ggttgtaact ccattcaatg gcagaatgca atgcagcgtg acgagcatga gcggcatcgg
     3121 ttgaggttaa gttcaacgac ttttccaagc gcgataagat gccatctaac gtgagattgt
     3181 tgacttgtgc agcggcaagc tcgatcgcta atggcatccc ttcgaggcgg cgacaaatct
     3241 ctgccacacg catcacattc tgaccgttga gctgaaagct gccattgacg gcccgtccac
     3301 gctcgacgaa gagccgcacc gattcgttct ttgcaagagt cttgagatga ctcgcgccag
     3361 gttcaggtag agggaatgct ggtgtttgcc agccaatttc acttgcaaca ccaagcggct
     3421 cacggctggt agcaagaaag gtcactgcct ggcatttggc ggatacggct tcgatactgt
     3481 gagcagtagc ggagacaaga tgttcacagt tatccaagat gattagcatc ttgcgttcgc
     3541 cgataaaact tgcaatgtgg tcaagcatat cggcgccgag cttttctttt accccaagcg
     3601 atttcgcaat ggcttgcggc aattggtctc ctttgagcag gggagcaagg tcaacccacc
     3661 agaccccgtc aggatagtta tgtgcagact catcagctgc ttgaatggca agccgggtct
     3721 tgccacttcc acctgcaccc aataaggtaa ggaagcgagt ttctgctaat aaggtttcta
     3781 cttcattcag ttcttcttcg cgcccaataa agctggtcaa tgggcggggg aggttgcctg
     3841 ttatatgcaa agatgcaggt gtcttttgca ggcggcgata aataccttgt aactctggcg
     3901 atggctctac gccaatttct cgtagagctt tactgcacga ttcgtattgt aagagcgctg
     3961 cttcccgatc tccaagtgac tcataacaga ggatgatcag ttggtgcgtg gactcacgga
     4021 cgggttcaat ctctagcgcg aggcgagcgg tttcaattgc agccacatac tgaccctgac
     4081 ctctttgatg ttgagcaagg cgtaaacggg tttcaaggca agcttgttca aattctgccc
     4141 gccattccaa tacccaatct tcataaactt gtggaagtag atccccagta tataaactca
     4201 gtgcagattg ccaatcttct ggcgtagcac gcgagtcagt taggcgttga aactcgcgta
     4261 catcaatcca taggtttaag tcagggttaa gttgcaacga gtcgcgaccg ccaattaggc
     4321 tatcttcgcc gagagtctta cgcaagtcgt tgatcgtcac acgaagcgaa agcctggcag
     4381 cttcgtcggt agtagcatct ttccaaaata tggaggcaat ccgttcgcgc ggatgctctt
     4441 gcgggtggag ggcaagatat gccaataaga gtaaagtttt ttggcgcgcg aactcaagag
     4501 tttccttccc gcgttcaagg tggactgacc caagcaagta aattttgatt gtaggtgact
     4561 gtgacatatt aaaagaggaa atcaaagatt attaagatgt gttttttaca gagtgtacca
     4621 tgccccctta atagggtcgc cattccaaat cttttaatgt gaatcgttat aatttcttca
     4681 tgtcgcaggc atacaggatt cgcatttttt tatttggatc gcctaggatt gaagttaatg
     4741 gcgttatatc tgccctgcct gcacgcgata aattactatg tctattcacc cgacttgtcc
     4801 ttcaggcgaa ttccccacat gggcggaaac atttggcatt ctcactctgg gcagatgagg
     4861 atgaagccag ttctcttgcg aatttaaggc ggcatcttta cctcctgaaa aaatctcttc
     4921 caaaagccct acaaccatca ttgatcatct ctacacaaac ggttttattt caattccctg
     4981 ctaacacctg ggtggatgtg cttgcgtttg aacaggaacc gacttctttg caagaaatag
     5041 aagaacacgc cctgttttac acaacagacc ttgcacaagg ggcagaaaca gatgagttca
     5101 ttttttctcg tcgcgaagaa ttgcgtaatc gatatgcaca gcttttgaga atgctggcac
     5161 agacatactt caactacgcc gaatacgaga catctatgta ttgggtgcgt aaactgattg
     5221 ctcaagaccc atgggatgaa gaagccctgc ggttgtatat gacttcagac ttcattcttg
     5281 gtaatcgcac aactgctctt tcaacttata aatcatttga gaaaaagttg aggctggaaa
     5341 tggacatcca gcccatgcca gaaaccatgg cactataccg agatattttg cataaccacc
     5401 ttttacgtcc tgtttcttcc acaaaaacag aaggtgaaac attcattagc cggttaagtg
     5461 aaatacaaac attgggaaat gcgatccagg cacatgggca atacggcaat tttgtgatca
     5521 tcaatggaac agccggtgtg ggaaagacca ctttggtccg tgaagcgctt cggcgatttt
     5581 taaattcatc aaaagccaaa ctactttggg gagattgcca gcctgcgggg gaagaacaac
     5641 cttactcgat ttggcagcaa atttttaatg ccgcagtccc tttgcttgcg cgtcaacagg
     5701 atgtggctca ggagtggcta aatcatttac tgccgctcat cccagacctg ggcttgttaa
     5761 gagccgatct gttttcccct acccgtccag atgctaccga attgcgcacc gctatgaggc
     5821 aaactttatt tgccttgtca gagagccgtc cgcttatcat agtgattgaa gatgcacatt
     5881 ggatggatgt cccatcctta gagttcttaa gagagcttgc tgaaagctct ctaaacattc
     5941 ctgttctttt tattcttacc catcgcacag atgatgctgc attgcctttg tttgaaatca
     6001 aacggatatt acgccgtaag cgaaatgtag tagatatctc tctgcaacca tttacaacaa
     6061 cggagacaaa tcaatatctt gaaaaatttt tgggaacaaa gactcttcca aaagaactta
     6121 tagatgacct catgcaatac tcaggtggac tgcctttatt attgcatgag gtgacccaaa
     6181 ccctcaactc aagcaagaga atcctttctt tgcgcgaggc gtttctcttt cggttagatc
     6241 gtctctcacc ggctgcacgt gaaatggtag agacggctgc tgtgattgga ttttccctga
     6301 ccgacattga attgcgtacg gctctgggtt gggcagcgga actttacgcc tccacaatgg
     6361 atgctttgca agcagaacgg tttcttttgg aaaatccttc aaatagtgca gacgaatatg
     6421 ctttttccca ccgcctgatt catgaaatta ttgtagagca gatctctgta gagcgaattt
     6481 caaatttaca tattcgtgtg gcacgcgcat tggaacaaat ccattcagaa gatattgggt
     6541 ttgcggcaga gatcgccctt cattatcagc aagccggtca gcttatcttg gctgccggct
     6601 tctgggtgcg ccatgctagc gaaagtacag acctggctgc atttgattct ggtctcgctg
     6661 ccatcaacca agccgaagcc attcttactg gcgcaagttt agatgctcaa gaggtgcacg
     6721 ctaaacttgc tcttcaacgc ggggtgatcg tgttttatca gggaaaaagc gaagaggctt
     6781 tttctttact tgaaaaagca gtggaatatt caaaac
//
LOCUS       DAOQJE010000061         6784 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_6245_length_6784_cov_7.636053, whole genome shotgun sequence.
ACCESSION   DAOQJE010000061 DAOQJE010000000
VERSION     DAOQJE010000061.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 6784)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6784)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6784
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6245_length_6784_cov_7.636053"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            304..1437
                     /gene="dnaN"
                     /locus_tag="PLT08_02620"
     CDS             304..1437
                     /gene="dnaN"
                     /locus_tag="PLT08_02620"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HNE03386.1"
                     /translation="MKVTVLQENLARGLSIVSRAVSSRSTLPVLSNILIATDEGRLRL
                     SATNLELGITCWIPARIDQDGSTTVPSRTFSDLVNTLPGDQVQLTLDLKTQNLHVKGG
                     SSNNDIKCIDAQEFPPMPTPEMKDAVQLNVADFKEMIQQVVFASSTDEARPVLMGVLM
                     QVEKDKVTMAAADGFRLSVRKGQLAHSVKETLNIIIPSRALNELSRVAHDPEEPIYMV
                     VPKQRGQVLFRVKDVEVVSQLIDGTFPDYHQIIPRNYKSRTLVSTAALLKACKQAEIF
                     AREGSNVARLDIKQSNGDMQPSEVEISATSEETGKNETIVEATVDGSGVLIAFNVKFL
                     REALEVIKTPNVALETTAQNAPGVIRPVGDDNFLHVIMPMHLG"
     gene            1568..3943
                     /locus_tag="PLT08_02625"
     CDS             1568..3943
                     /locus_tag="PLT08_02625"
                     /inference="COORDINATES: protein motif:HMM:NF013491.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PEP/pyruvate-binding domain-containing protein"
                     /protein_id="HNE03387.1"
                     /translation="MTLPRVSIDNVLRILLSLSQYPILSSRIRHQMRKTLYSRGIVRK
                     EVFEEEVKRKAVESQTLEGITDPLTEESNEVWLMRITRVKDSLTDFYFAYNLPYAEFE
                     DLVRKVLAERGSIDSDFVWVNPELAPQELLFEQAEMIESMPDADKEKYEARLQAIIAV
                     LIRTMISDQLKYIKIARQWFTVEDLREINRRKIGGGKIGGKAAGMLLAMRILKETAPV
                     QIRNRFRLPISYYLGSDVYYNFMSLNDLGGWNGQKYKTEEQMRADYPELCEDFYKGQF
                     PPEVIESLERVLEEAGQRPLIVRSSSLLEDNFGTSFAGKYESIFLPNQGDANECLKAL
                     TQAIAKIYASVMNPDALIYRHTKELADYDERIGILIQIVEGERAGRYYFPHGAGVAFS
                     RNLFRWSPQIKQDEGFLRLVTGLGTRAVDMLADDYPRLVALSHPRLHSSSDVRTIKRY
                     SQQHLDAIDIETNSFVTIPVRDALHTDYDPLRYIAQVEQDGYLAPIRSRLDSTDKLVI
                     TFDGLLSRTPFSKSMRAALTLLETHYGSPVDTEFTVEIVNPDSQPDVRITILQCRPQS
                     HIHEANEVQIPSDLETQDIVFSSQTMVPQGVVENIGYVLFVPSEGYFSLETQAERTQL
                     ERAIGQLNAALKGQTFIAVGPGRWGTSTPDLGVHVAYSDIYNSRALVELAGEEIGASP
                     EPSFGTHFFQDLMEANIYPLGVFVDEEGTIFKRDFFYSTPNRLSEFISIENPRVVAAL
                     KLIAVSDFRPNTHMNLIMEASKSRAVAFLVGKATPIESDDAIVGTAPTSLE"
     gene            complement(4000..4920)
                     /locus_tag="PLT08_02630"
     CDS             complement(4000..4920)
                     /locus_tag="PLT08_02630"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014566.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HNE03388.1"
                     /translation="MIEKKNIYWFLGITVLLSWPLFLSTLFLGNMDAQTKQLTTQGLW
                     AAAMWGPGIAAIITTLFIAKVPFNSLRLNTIGPKRFYLWAWFLPTVFTVLSGLFTILF
                     GIGKLDLSFSMIRTALESTPGGSEIPASFVVLSQSLLALTLAPFLNMLFTMGEELGWR
                     GFLLPRLLPLGQWKAVLWSGLIWGLWHAPVIVQGHNYPGYPILGVFMMIVFCFLLGTI
                     IAWLYINTKSPWVAALAHGAVNAIAGLPVLFLAPGFNMAFGGTLAAPTAWIGMALFIA
                     WLVWTKRFPVQEQTGDAQSTSEVDRSENTK"
     gene            complement(4932..5171)
                     /locus_tag="PLT08_02635"
     CDS             complement(4932..5171)
                     /locus_tag="PLT08_02635"
                     /inference="COORDINATES: protein motif:HMM:NF025156.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4177 domain-containing protein"
                     /protein_id="HNE03389.1"
                     /translation="MNNNELSKWEYRVQTVGNFWSGVKATELEELLNAWGEEGWEVVS
                     THVLENQNKINVIAKRPLTRETIRQRSMPNYQYEK"
     gene            complement(5236..5886)
                     /locus_tag="PLT08_02640"
     CDS             complement(5236..5886)
                     /locus_tag="PLT08_02640"
                     /inference="COORDINATES: protein motif:HMM:NF025015.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1648 domain-containing protein"
                     /protein_id="HNE03390.1"
                     /translation="MSTKTTSILVIALVAIAVIAGVILWNQLPDQMASHWNVNDQADD
                     YMPKFWGVFLMPIISLGMLALFLVIPNIDPLKANIAEFRNIFNLFILLIVVFMLYVHG
                     LTLAWNMGNQSFKMSAAMLPFLGVLFIFIGYMLRQAKRNFFIGIRTPWTLSSDTVWNK
                     THQLGSVLFMVSGAFAFIGSFLGGMLAFWLLFVPLMGSVVFLILYSYILYRNETRA"
     gene            complement(5922..>6784)
                     /locus_tag="PLT08_02645"
     CDS             complement(5922..>6784)
                     /locus_tag="PLT08_02645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="slipin family protein"
                     /protein_id="HNE03391.1"
                     /translation="VFGIILIGSIKVALILDANHASDLVTAAVMLAMIGIASYFLFAI
                     KVASQWEKAVVLRLGKFNGLKGPGLFWLIPIIDTAVMWIDHRVTVTSFSAEKTLTKDT
                     VPVDVDAVLFWVVWDAEKAALEVNDYQSAISWAAQTALREIIGQMNLADILVGRAKMD
                     EELQKIIDERTTPWGVTVQSVEIRDVVIPQVLEDAMSRQAQAERERQARVILGESEKQ
                     IATSFSEASQAYIDNPTALHLRAMNMLFEGLKEKGALVIVPSSAIDTMNLGGLSGMVS
                     LAQNNAPKEK"
BASE COUNT         1825 a         1605 c         1544 g         1810 t
ORIGIN      
        1 atccgcccgc agtcttccaa acgcaccgcc tctatttgga cggttgtagc ctatgttttc
       61 gcagccttgt ttgcgtgtca gttgttgttt gggttgctga tgtttggggt ctcgatggtg
      121 acaggcttct agtaaagaag tagcaaaaaa tttctacccg aaggagtgac atggcttgta
      181 aacgctgtgc tattccttcg ggatttgttt taattcgcga ttgttatcta taatccttcc
      241 atccctttgg gattggatga tatacttttg aaaattctga ctaattatta agcgaggcgc
      301 accatgaaag taactgtcct tcaagagaat cttgcccgtg gtcttagtat tgtttcgcgc
      361 gcagtttcct cgcgcagcac actccctgtt ctttcgaata ttttgatcgc gaccgacgaa
      421 ggtcgtttgc gactttcagc caccaacctt gagttgggca ttacgtgttg gattcccgct
      481 cgcattgatc aggatggttc aacgaccgtt ccctcgcgca cattttcaga tttggtgaat
      541 accttgccgg gcgatcaggt gcagttgacg cttgatctga aaacccagaa tctgcatgta
      601 aaaggcggct cctcgaacaa cgatatcaag tgtatcgacg cacaggaatt cccgcccatg
      661 cctacgccgg agatgaaaga tgccgtgcag ttgaacgtgg cagatttcaa agaaatgatc
      721 cagcaggtgg tttttgcttc ttccaccgat gaagctcgcc cggtattgat gggtgtgttg
      781 atgcaggttg aaaaagacaa ggtcaccatg gcggcagcgg acggctttcg cctttcggtg
      841 cgcaaaggtc agcttgcgca ctctgtgaaa gagacgctca atatcatcat cccttcacgt
      901 gcgttgaacg aactttcgcg tgtggcgcat gaccccgaag aacccattta tatggttgtc
      961 ccaaaacagc gcggacaagt ccttttccgt gtgaaagatg tggaagtagt ctcgcagttg
     1021 atcgatggga ccttcccaga ttatcaccag atcattccgc gcaattacaa gtctcgtacc
     1081 ttggtttcca ctgcggcttt gctcaaagcc tgcaaacaag cggagatctt tgcccgtgaa
     1141 ggttcgaatg tggcgcgttt ggatatcaaa caatcgaatg gcgatatgca gcccagcgaa
     1201 gtggaaattt ctgcgacctc ggaagaaact ggtaagaatg aaaccatcgt tgaagccacc
     1261 gtggatggta gcggtgtttt gattgcgttc aacgtcaagt tcttgcgtga ggcgctcgaa
     1321 gtcatcaaga ccccgaatgt ggcgctggag accacggctc agaatgcgcc gggagtcatc
     1381 cgcccagttg gtgatgacaa cttcttgcac gtcattatgc cgatgcattt gggataggct
     1441 tacaaacaga atacgaatcc gccagagacc tatctggcgg attttttgtt ataaaatcgg
     1501 acaatgaacc gcagatgatc gacctgcgtt ttgtctctat cgtcagttgt ccatcaaagg
     1561 tgaattcatg actctcccac gcgtttccat tgataacgta cttcgcatct tgctttcgtt
     1621 gagccaatat cccattctca gtagtcgcat tcgccatcaa atgcggaaga cgctgtattc
     1681 acgcggtatt gttcgaaaag aggtttttga agaagaagta aaacgcaaag ccgttgaatc
     1741 tcagacactt gagggcatta ccgacccgtt gacggaagag tcgaatgaag tttggttgat
     1801 gcgtatcacc cgtgtaaaag actcgctcac cgatttttat tttgcataca acctgccgta
     1861 tgctgaattt gaagatttag tccgcaaggt gttggcagaa cgcggcagta ttgactcaga
     1921 ttttgtttgg gtcaacccag aactagcgcc acaggaattg ctttttgaac aggcagagat
     1981 gatcgaatcc atgccagatg cagacaagga aaagtacgaa gcgcgtctgc aagccattat
     2041 tgcggtattg atccgtacta tgatcagcga tcaattgaaa tatatcaaga tcgcgcgtca
     2101 gtggtttact gtggaagacc tgcgcgagat caatcgtcgc aagatcggcg gcggaaagat
     2161 cggcggtaaa gcggcgggca tgttgcttgc catgcgcatc ctaaaagaga ccgcgccggt
     2221 acagatccgc aaccgtttcc gtttgcccat ttcttattat cttggctccg atgtgtacta
     2281 caacttcatg tcgctcaatg accttggtgg ttggaatggg cagaaatata aaaccgaaga
     2341 acagatgcgt gcagattacc ccgagctgtg cgaagatttt tacaagggac aatttccacc
     2401 tgaagtgatc gaaagccttg agcgcgtttt agaagaggca ggtcaacgcc cgttgatcgt
     2461 gcgttcctcc agtttgcttg aagataattt tggcacatcg tttgctggaa aatatgaaag
     2521 cattttcttg cctaatcaag gggatgctaa tgaatgcctc aaggcactca cgcaagctat
     2581 tgccaagatc tatgccagtg taatgaaccc cgatgcgctg atctatcgtc ataccaaaga
     2641 acttgctgat tatgatgaac gcatcggtat tctgattcaa atagttgaag gcgaacgagc
     2701 tggtagatat tactttcccc atggggctgg tgtggcattt agccgaaacc ttttccgctg
     2761 gtctccgcag atcaaacagg atgaaggctt cttgcgtctt gttactggtt taggtacccg
     2821 cgccgtcgat atgcttgcag atgattatcc gcgattagta gcgctcagtc atccgcgttt
     2881 gcattcgtct tcagatgtcc gcaccatcaa acgctattcg caacagcacc ttgatgcaat
     2941 tgacattgaa acgaattctt ttgtaaccat ccccgtgcgc gatgctctcc acacggatta
     3001 tgatccgctg cgttatatcg cccaagtgga acaagacgga tacctcgctc cgatccgttc
     3061 gcgcctcgac tctaccgata aactggttat taccttcgac ggcttactct cgcggacgcc
     3121 tttctcaaag tccatgcgag cggcattgac tttgcttgaa acacactatg gttcgcctgt
     3181 cgatacagag ttcaccgttg aaatagtaaa ccctgatagc cagccagatg tccgcattac
     3241 gattttgcag tgtcgtccgc aaagtcatat tcacgaagcg aatgaagtac agattccaag
     3301 tgaccttgaa acacaagaca tcgttttctc gtcacaaacc atggtgccac aaggggttgt
     3361 ggaaaatatc gggtatgttt tgtttgttcc ttcagaaggt tatttcagcc ttgaaacaca
     3421 agcagaacgt acccaactgg aacgcgccat tggtcaactc aatgcggcgt tgaaaggaca
     3481 aacctttatc gcagtaggac ctggacgctg gggaacatcg acaccggatt tgggtgtgca
     3541 tgtggcgtac agcgatatct ataactcgcg tgccttggtt gaattggcgg gagaagagat
     3601 cggagcatct cctgaacctt ctttcggcac ccatttcttc caagacctta tggaggcgaa
     3661 tatctatccg cttggtgtat ttgttgatga agaaggaact attttcaagc gtgacttttt
     3721 ctatagcacc ccaaatcgac tttctgaatt tatctctatt gaaaatccac gagttgtcgc
     3781 tgctttgaaa ttaattgctg tttcagactt tcgtccaaat actcacatga atttgattat
     3841 ggaagcaagc aaaagtagag ctgtcgcttt tctagttggg aaagctactc caattgaaag
     3901 tgatgacgca attgttggaa cagcaccgac aagtttggaa taaaaaaatg ggcgactcat
     3961 cgtcgaccat tttttataat tacaaaaaga acaaatgttc tattttgtat tttcactcct
     4021 atcaacttct gatgtcgatt gtgcatcacc tgtttgttct tgtactggga aacgtttcgt
     4081 ccacaccaac catgcaatga acaaagccat gccaatccat gcagttggtg ctgcaagtgt
     4141 tccaccgaat gccatattga aaccaggtgc caggaacaat acaggcaacc ctgcaattgc
     4201 attgactgct ccatgtgcaa gcgctgcaac ccatgggctt ttggtattga tatacaacca
     4261 tgcaatgatc gtcccaagca agaaacaaaa tacgatcatc atgaacacac ccagtatcgg
     4321 atacccagga tagttatgtc cttgtacgat cacaggtgca tgccacaagc cccatatcaa
     4381 accactccat aaaacggctt tccattgtcc gagtggcaac aatcgcggaa gtaaaaatcc
     4441 acgccagcct aattcttctc ccatcgtgaa tagcatgttt aaaaatggtg caagtgttaa
     4501 tgccagcaat gattgagata agacaacaaa gcttgctgga atttcacttc cacccggcgt
     4561 agactctaat gcagttcgta tcatgctgaa gctcaaatct aatttgccta ttccaaatag
     4621 aatagtgaag agtccactca gcacagtgaa tacagtgggc agaaaccatg cccaaagata
     4681 aaagcgcttt ggtccaatcg tattcaaacg caaactattg aatggtactt tggcaatgaa
     4741 caaagtagtg atgatggctg caattcctgg accccacatt gcagcagccc ataaaccttg
     4801 agtggttagt tgtttagttt gggcatccat atttccaagg aacaatgttg aaaggaataa
     4861 cggccatgaa agcaacactg taattcctag aaaccaatag atgtttttct tttcgatcat
     4921 tttatatctc cttacttttc gtattgataa ttcggcatcg aacgttgacg gattgtttcg
     4981 cgagtcaacg gacgtttcgc gatcacattg attttgttct ggttttcaag cacatgtgtg
     5041 gatacaactt cccagccttc ttcaccccat gcgttcaata gttcttccaa ctcggtggct
     5101 ttcaccccag accaaaaatt cccaacggtt tgtacgcgat attcccattt ggataattca
     5161 ttattgttca ttggaaccct ttcgtttcac gtgaaacaaa acgtgcgtgt ctgtaccaga
     5221 cacgcacgtt ttgttttatg cacgagtctc gttacggtac agaatatacg agtacaggat
     5281 caagaatacg acggagccca tcaacggaac gaataacaac cagaacgcaa gcatgccacc
     5341 aagaaaactt ccaatgaagg cgaaggctcc tgaaaccatg aacaagaccg agccgagttg
     5401 atgcgtttta ttccacaccg tatcacttga aagagtccat ggcgtgcgta tcccaatgaa
     5461 gaagttgcgc tttgcctgtc tcagcatata gccgatgaag atgaacaaaa ccccgagaaa
     5521 tggcaacatg gcggcactca tcttgaaact ttgattgccc atgttccatg ccagtgtgag
     5581 cccatgtacg taaagcatga agacaacaat gagcaaaatg aacaggttga agatgttacg
     5641 gaattccgcg atgtttgcct tgagcgggtc aatattgggt atgacgagaa acagcgccag
     5701 cattcccagt gagatgatcg gcatgaggaa aacgccccag aactttggca tgtaatcatc
     5761 tgcctgatcg ttgacattcc agtgagaagc catttgatct gggagttgat tccataagat
     5821 aactcccgcg attacggcaa tcgcgaccaa agctataaca agaatagaag ttgttttcgt
     5881 tgacattctt ttcctttctt cattcgtttc acgtgaaacg tttacttttc ttttggcgca
     5941 ttgttttgcg ccaatgaaac catgccgctt agcccgccga gattcatcgt atcaatcgca
     6001 gaagaaggta cgatgaccag agcgcctttc tctttcaacc cttcaaagag catattcatc
     6061 gcgcggagat gcaaggcagt tgggttatca atataagctt gcgaagcttc agagaaagat
     6121 gtggcgatct gcttttcaga ttcaccaaga atgacacgtg cctgacgttc gcgctctgcc
     6181 tgggcttggc gagacatggc atcttccaag acttgaggaa taaccacatc gcgaatctct
     6241 acagattgca cggtcacgcc ccaaggtgtt gtacgctcat caatgatctt ctgaagttct
     6301 tcgtccattt ttgcgcgccc aacgagaata tctgctaaat tcatttgtcc aatgatctca
     6361 cgcaaagcgg tttgcgcagc ccatgaaatt gcgctctgat aatcgttcac ttcgagtgcg
     6421 gctttttctg catcccacac aacccagaac aatacagcat ccacatccac aggtacagtg
     6481 tctttggtca gagttttttc tgcagagaaa gatgtgaccg taacacgatg gtcgatccac
     6541 atcacagcag tatcaatgat cggaatgagc caaaacaaac cgggtccctt aagcccattg
     6601 aatttaccga ggcgcagaac aacagccttt tcccattgag atgccacttt gatcgcaaac
     6661 aaaaaataag aggcaatccc gatcatggca agcatgaccg cggctgtaac aagatccgat
     6721 gcatgattag catctaaaat taacgctact ttgattgagc caattaagat gatcccaaac
     6781 acaa
//
LOCUS       DAOQJE010000062         6708 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_6334_length_6708_cov_7.260785, whole genome shotgun sequence.
ACCESSION   DAOQJE010000062 DAOQJE010000000
VERSION     DAOQJE010000062.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 6708)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6708)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6708
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6334_length_6708_cov_7.260785"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..860)
                     /locus_tag="PLT08_02650"
     CDS             complement(<1..860)
                     /locus_tag="PLT08_02650"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: protein motif:HMM:TIGR01930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003985 - acetyl-CoA C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase"
                     /protein_id="HNE03392.1"
                     /translation="MSKENEAVILSAARTPIGKFQGALSGIPAPKLGAIAVEEAVKRA
                     GIDPSEVEEVFMGNVVQAGNGQAPARQAAIYGGIPATVSATAINKVCGSGLKAAMMSA
                     QAIRAGDAELFIAGGFESMSRAPYLVSGRMGELKFGNSQMTDALLNDGLWDPFENWGM
                     GNAAEFIADEYEVTRAAMDEFALRSHLKAVEAQEAGRFNAEIVPVQIPGRKGEVTTVA
                     HDEGPRKDTSLESLAKLKPVFKADGKVTAGNSSSMNDGAAATIISSRGYAEKKGLKPL
                     ARIIGYAQAA"
     gene            1028..3907
                     /locus_tag="PLT08_02655"
     CDS             1028..3907
                     /locus_tag="PLT08_02655"
                     /inference="COORDINATES: protein motif:HMM:NF025056.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIR domain-containing protein"
                     /protein_id="HNE03393.1"
                     /translation="MSDVFISYSRRDKVFTQKLYEALRDSGREIWADWDSIPAASDWF
                     AEIKDGVEKANTIVFVLSPEWLKSNECRKEYDHAVAMGKRLLPILYQVVDPKEVPSEL
                     AKINWVYMRDTDDFGQAFQTLSAAMDTDLEWVKTHTRIQIRAIEWDKKSRDHSFLLRG
                     NDLTDGEQFIAQGAQKSPEPTVLHGEYVLASRKDATRRQRITLAGVTVALVVSVALGI
                     VAFFQRQAAVAARADAVVERDRAVAAEKIAEDERDRADVNAEVAYSRELAAASLNSLN
                     HDPELSLLLGLQSVAITDKTGMPIQPMSEDALRRSVQTSRIRKTWLAHSGSVLDIAIS
                     PDGTKIASASDDLTVKIWDLSGKLLLTIPDFSEEVDSVVFSPDGSQILTSSGQEAVAW
                     DAVTGEKLVEYKGHVSYVVQAVYSPDGKFIATASSDATAIMWDALSGESLVVLGGHET
                     ELSDIAYSPDGEMIASTDYNGAMYVWSTSSKSLLYMLSEGEGSVILDVEFSPDGKYIL
                     ISDNYFQTIVLVAQTGKVISAQNVHTDFVTNAVVSPDGAFMASASTDNTVVVSSITAS
                     AVLFGQSISADPDYIDISKVMFRFTDSTDINTMIYTPDGKYLITGNDEGQIKIWDANP
                     ISDYEVSGFRPHTARIDGMEFSPDGEHYVTVARDGKAFIWSLTDQSSIELVGHQNVIQ
                     DVAYSSDGMQIITGGFDGHAIIWDAKTGDKLITLSKDDQLVTGVNFSPDGKLAVGSLG
                     DNTVVVWDAVSGDEVRVLQSKDMYAYINELAFSPDGKTLAVSSDAGFVVLWDVATWNQ
                     IDVLAGHTDWVKGLDYSSDGTHMVTASYDTTAIIWDVATRTPVLKLEGHSQGVNDVTY
                     SPDDTLVATASVDGTAKLWNANTGELLVTYQTSADQILSVAFSADGKYLATGSETGVV
                     RIYYVYAEDALELAKSRITRTFTEEECLKDLHEEACPANLPQPQQ"
     gene            3972..4385
                     /locus_tag="PLT08_02660"
     CDS             3972..4385
                     /locus_tag="PLT08_02660"
                     /inference="COORDINATES: protein motif:HMM:NF013331.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II truncated hemoglobin"
                     /protein_id="HNE03394.1"
                     /translation="MTEQSLYELLGGEEGVRTLVDRFYDLMDSSPEAKDVRALHAKSL
                     KQAREKLFMFLSGWSGGPPLYIEKFGHPRLRMRHMPFVIGTRERDQWLWCMNRAVDAG
                     NYHSAVVEHLKQRFSEIADFMRNQDETEQGPLLQL"
     gene            complement(4382..5056)
                     /locus_tag="PLT08_02665"
     CDS             complement(4382..5056)
                     /locus_tag="PLT08_02665"
                     /inference="COORDINATES: protein motif:HMM:NF020296.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HNE03395.1"
                     /translation="MHPYVRSLKILFEANANPVNAAPMKKYMRDQFEYLGIKSPQVKS
                     LFSGYIKQNSLPPVEDLSTIVRELWNLPQREFQYLAINLLDKMEKKLPEDFIVTLEYL
                     ITHKPWWDTVDLIAGHSVGMMFKRYPKVKAQYLKKWRKSENIWLRRTTLLFQLGYKKE
                     TDFGLMCELIQENLGSDEFFINKAIGWALRQYAWTNPSAVKKFVKATKELSPLSQKEA
                     LKNIGT"
     gene            complement(5068..>6708)
                     /locus_tag="PLT08_02670"
     CDS             complement(5068..>6708)
                     /locus_tag="PLT08_02670"
                     /inference="COORDINATES: protein motif:HMM:NF037564.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SdrD B-like domain-containing protein"
                     /protein_id="HNE03396.1"
                     /translation="WGAINFGAFILKRMTSLFWVLFLLVACNSNATVETPIENTPIIS
                     ITPSTTSTPIFTPTPQPHSQPVTYGPDRENFPSNVNPLSGLQVENPSLLQTPAMALSI
                     SNFPVAARPQAGLSFAPWVFEFYITEGATRFLSIFHGTFPEPEAPIVGDCEVRREPIQ
                     GSVIEIGNRIWLDENQNGIQEDYEKGVGGVCINLLDATGKSVQKTTSDSNGFYGFVTE
                     PGNYIVEVILPAWLKFTKPNVGDEEHDSDADPVSGKIEADLISTPLDLDVGLVPSEKA
                     NPPVKQSAQLPPAEVGPIRSGRLLYVDVHSMFLNSCFVFASASEEILPLLPQCYLVPH
                     DEASGGAMLSIEKMKSLAEENKGTRPVDFNYASNVFAETPPEGGETALRLNSYVALLN
                     QSAWVYDPASSSYWRYVDDTTQANAGVLHPEIDRLTGRQLQFNNIIVLEVDQEVYEYQ
                     DRPIRTLLDYHLELGQGGYAYLFRDGRKYDIRWTTKARAYEKQTGQARPLYFVDANGD
                     PVSLKPGNTWFIVATPYSYFTPEDGVWNFRFVPPEGVK"
BASE COUNT         1706 a         1476 c         1673 g         1853 t
ORIGIN      
        1 attgctgcct gggcatagcc aatgatcctt gcaagcggct tgagcccttt cttttcagca
       61 tatccacgcg aagagatgat cgtcgccgcc gcgccatcgt tcatcgacga tgaatttcca
      121 gccgtcactt ttccgtccgc tttgaagacg ggtttgagct tggcaagtga ctctagcgat
      181 gtatccttgc gcggaccttc atcgtgagcc actgtggtca cttcaccttt gcgcccggga
      241 atttggacgg gaacgatttc agcgttgaaa cgtcccgcct cttgagcctc tacagctttg
      301 aggtgagagc gaagcgcgaa ttcatccatc gcggcgcgag tcacttcata ttcatccgcg
      361 ataaattctg cggcattgcc catgccccaa ttttcaaacg gatcccacag accgtcgttg
      421 agcagggcat cggtcatttg ggagttgccg aatttcaact cgcccatgcg cccgctgacg
      481 agatacggag cgcgactcat cgactcaaac ccaccggcga tgaagagctc cgcatcaccc
      541 gcccgaatgg cttgagcaga catcatcgcc gctttcaagc ccgagccgca gaccttgtta
      601 atggcagtcg cactgacggt tgcagggatg ccgccgtaaa ttgccgcttg tcgtgccggt
      661 gcctgaccat tgccagcctg caccacattg cccatgaaaa cttcttccac ttcgctgggg
      721 tcgatgcctg cgcgtttaac cgcttcttcc acggcaattg caccgagttt cggcgctgga
      781 ataccgctca acgcaccttg gaattttccg atcggtgtgc gggctgcgct caatataacc
      841 gcttcatttt ctttggacat cgtacctcct gtggggtgat attcgtatta tacgcgagag
      901 ccgccatagg gcagaaattc tgttaagttc tttcattctc tgctttcaaa cttgtaggtc
      961 tttccataac aaagcctact atataatcga cacatttctt atggatatat aggatcagga
     1021 gagcgacatg tcagatgtat ttatttctta ttcaaggcgc gacaaagtat tcactcaaaa
     1081 actatatgaa gcactaagag actctggtcg tgagatttgg gcggactggg actcgatccc
     1141 agcggcatcg gattggtttg ctgagatcaa agatggggtg gaaaaagcaa acacgattgt
     1201 ttttgtgtta agcccggaat ggttgaaatc gaacgaatgc cgcaaagaat atgatcatgc
     1261 cgtggcaatg gggaaacgat tattgcctat cctatatcaa gtagtagacc caaaggaagt
     1321 tccgtctgag ctggcgaaga ttaactgggt ctacatgcgt gatacagatg attttggtca
     1381 ggcattccaa accctttctg ccgccatgga tacagacttg gaatgggtaa aaacccatac
     1441 tcgtattcaa atccgtgcga ttgaatggga caagaaaagt cgtgaccatt cattcttatt
     1501 gcgtggaaat gacctaacgg atggagagca atttattgct caaggggcac agaaatcacc
     1561 ggaacctact gttctccacg gtgaatatgt gcttgccagc cgaaaggacg ccactcgccg
     1621 tcaacggata actttggcag gtgtaacggt tgcactggtt gtttcggttg ctttgggcat
     1681 tgtggcattt ttccagcggc aggcggctgt tgctgcgcgt gcagatgctg tggtggaaag
     1741 agatcgtgct gtggctgccg aaaaaattgc ggaagatgaa cgagatcgtg cggacgttaa
     1801 tgcagaagtt gcatattctc gtgagctagc cgctgcttct ttgaattcat taaaccatga
     1861 ccctgaactc agtctgttgt taggtttgca atctgttgct attacagata aaacggggat
     1921 gccaattcaa cctatgagtg aagatgccct gagacgttct gtgcaaacat cacggattcg
     1981 aaaaacctgg ttggcacatt caggttcagt tctcgatatt gcaattagcc ctgatggaac
     2041 taagattgca tctgccagtg atgatctaac agttaagatc tgggacttgt ctggtaaatt
     2101 attattgacc atccctgatt tttcagaaga agtcgatagc gttgtgttta gtccagatgg
     2161 ttcacagatt ctgacctcaa gcggacagga agcagttgca tgggatgctg tcacaggcga
     2221 aaaattggtc gaatataaag gtcatgtatc ctatgttgta caagcggtct atagcccaga
     2281 cgggaaattt attgccaccg ctagttcaga tgcaactgca attatgtggg atgctttatc
     2341 tggagaaagc ttggtagtgc tcggtgggca tgaaacagaa ttgtctgata tcgcatatag
     2401 ccctgatggc gaaatgattg cctcgacaga ctataacgga gctatgtatg tttggagcac
     2461 cagttcgaag agtttattgt atatgctttc agaaggggaa ggttctgtta tccttgatgt
     2521 agaatttagc ccagatggta agtacattct tatttctgat aattattttc aaacgattgt
     2581 tcttgttgcc caaactggca aggtaatctc agcgcagaat gttcataccg actttgtgac
     2641 caatgctgtg gttagcccgg atggagcatt tatggcaagt gcctctacag acaataccgt
     2701 ggttgtaagc tcaataactg caagcgcagt attgtttgga caaagtattt ctgccgaccc
     2761 agattatata gatattagta aggtcatgtt caggtttaca gattctacag atattaatac
     2821 tatgatctat acccctgatg gaaagtacct cattacagga aatgatgaag ggcaaataaa
     2881 aatatgggat gcaaacccaa taagtgatta tgaggtgagc ggattccgtc cacatacagc
     2941 acgtattgat ggcatggagt ttagcccaga tggtgaacat tatgtcactg tagctcgaga
     3001 tgggaaggcg tttatctggt cattgacgga tcaatcgtcc attgaattgg tcggacacca
     3061 gaacgtcatt caagatgtag cctatagctc tgacggaatg cagataatca ctggcggttt
     3121 tgatggtcat gccattattt gggatgctaa aacaggcgat aaattaataa cactttctaa
     3181 ggatgatcag ttggttactg gggttaattt tagccctgat ggtaaattgg ctgtaggctc
     3241 attgggtgat aatacagtag tggtttggga tgctgtatct ggtgacgaag tacgtgtttt
     3301 gcagtcgaaa gatatgtatg catacatcaa cgaattagct ttcagcccag acggtaaaac
     3361 tcttgctgta agtagcgatg ccggatttgt tgttctatgg gatgttgcta catggaatca
     3421 aattgatgta ttggctgggc ataccgattg ggtcaagggc ttggattaca gttctgatgg
     3481 tacgcatatg gttacggcaa gttatgacac cacggcgatt atttgggatg ttgcaacgcg
     3541 gacccctgtt ttgaaattgg agggacattc ccagggagtg aatgatgtga catacagccc
     3601 agatgatacg ctggtggcta ctgcaagtgt agatggaacc gccaagcttt ggaacgcaaa
     3661 tactggggag ttattggtca catatcaaac ctcggcggac caaatcttaa gtgtggcatt
     3721 tagcgctgat ggaaaatatt tggcaactgg cagcgagacg ggcgtggtgc gtatctacta
     3781 tgtatatgct gaagatgccc ttgaactcgc caaaagcagg atcacgcgta catttacaga
     3841 agaagaatgt ttgaaagatt tgcacgaaga agcttgcccg gcgaatctcc ctcagccaca
     3901 acagtaacta aagtaaaccg ccttctagta gatagaaggc ggtttttatt tctttcgatt
     3961 acaatgccgc tatgactgaa caatcccttt atgaattact cggcggtgaa gagggtgtgc
     4021 gtaccctggt tgaccgcttc tatgacctca tggattcttc gccggaagca aaagatgtac
     4081 gtgctttgca tgccaaaagc ttgaagcaag ctcgcgagaa gttatttatg tttctctctg
     4141 gctggtcagg cggacctccg ctatatatcg agaaatttgg gcatcctcgt ttacggatgc
     4201 ggcacatgcc gtttgtcatt ggtacgcggg aacgtgacca gtggttatgg tgtatgaatc
     4261 gcgccgtgga cgcagggaat tatcactcag ctgtggttga gcatctcaaa cagcgcttct
     4321 ccgaaattgc agacttcatg cgcaatcagg atgaaactga acaaggtccc cttttgcagc
     4381 tttaagtgcc aatatttttc agcgcttctt tttggctaag tggacttaat tcctttgtgg
     4441 ctttcacgaa ttttttcaca gcgctgggat tggtccacgc atattggcgt aatgcccaac
     4501 cgatggcttt gttgatgaag aactcatcag agccaaggtt ctcttgaatt agctcgcaca
     4561 ttagtccaaa atctgtttct tttttatagc ccagttgaaa caacagagtg gtgcgtctga
     4621 gccagatgtt ctcagatttg cgccactttt tcaaatactg tgccttgacc tttgggtagc
     4681 gcttaaacat catgccaacg ctgtgccctg caatgaggtc gaccgtgtcc caccagggtt
     4741 tgtgggtgat aaggtattca agcgtaacaa tgaaatcttc aggtagtttc ttttccattt
     4801 tatcgagcaa gttaatggca aggtactgaa actcgcgttg agggagattc cataattcgc
     4861 ggacgatggt gcttaagtct tctacaggcg gcaaactgtt ttgcttgatg tatccagaaa
     4921 acagactctt cacttgcggg ctttttattc ccagatactc aaattggtcg cgcatatatt
     4981 tcttcatcgg cgcggcgttg acggggttgg cattggcttc aaaaagtatt ttcaaggaac
     5041 ggacataagg gtgcatagtt tcctctttta tttcacacct tcaggcggca caaaacggaa
     5101 attccaaacg ccatcttcgg gcgtaaagta cgaatagggc gtggcaacga tgaaccaggt
     5161 attgcccggc tttaaggaga ctgggtctcc attcgcatct acaaaatata acgggcgggc
     5221 ttgcccggtt tgtttttcat aagcacgggc tttggttgtc cagcgaatat catattttct
     5281 gccatctcga aagagataag catacccacc ttgcccaagt tcgaggtgat agtctagcaa
     5341 ggtgcggatg ggtctatcct gatactcata gacttcctgg tcaacttcca atacgatgat
     5401 gttattgaat tgtagctgcc tgcctgtcag gcggtctatt tctggatgta agactccggc
     5461 atttgcttgt gtcgtgtcat ctacataacg ccaatacgaa ctggaggcgg ggtcatacac
     5521 ccatgcagat tgattgagta aggctacata agaatttaaa cgcagcgctg tctcaccacc
     5581 ttcgggtggc gtctctgcga aaacattgct ggcgtaattg aaatcaaccg gtcgtgtgcc
     5641 tttattctct tctgccagcg atttcatctt ttcaatgctt agcattgcac cgccactggc
     5701 ttcgtcatgt ggcaccaaat agcattgtgg caacaatggc agaatttctt ccgatgccga
     5761 tgcaaagaca aaacatgaat ttaagaacat gctgtgaaca tccacgtata acaagcgtcc
     5821 cgagcggatg ggtcccacct cagcgggggg gagttgggct gattgcttca cgggtggatt
     5881 cgctttttcg gacggaacca agcccacgtc taagtcaaga ggcgtggaga taagatccgc
     5941 ttcgatcttc cccgaaacag ggtccgcgtc actgtcatgt tcctcatctc caacatttgg
     6001 tttcgtaaat ttcaaccacg ccggaagaat cacttcaacg atatagtttc ccggctctgt
     6061 gacgaagccg taaaaaccgt ttgagtcgct ggttgttttc tgaactgatt tcccggtcgc
     6121 atccaataaa ttgatacaga ctccccctac acctttttcg taatcttctt gaatgccgtt
     6181 ttggttttca tccaaccaga tacgatttcc aatctcaata acactgcctt gaatgggctc
     6241 acgcctcact tcgcaatcgc caacgatggg agcttctggc tcgggaaatg ttccatgaaa
     6301 aatggagagg aaccgggttg cgccttcggt gatgtaaaac tcaaagaccc atggcgcaaa
     6361 cgataaccct gcctgtgggc gagctgccac gggaaaattt gaaatggaga gtgccatggc
     6421 tggggtttgt aataagcttg ggttttcaac ctgcaaccca gaaagcggat tcacattgga
     6481 cggaaaattt tcacggtctg gtccataggt gactggttga ctgtgaggtt gaggggttgg
     6541 cgtgaatata ggagttgagg ttgtggatgg tgtgatcgaa ataataggtg tattttcgat
     6601 gggtgtttca accgttgcgt tactgttaca agcaacaagt aaaaacaaaa cccagaacag
     6661 tgatgtcatt cttttcaaga tgaaagctcc aaaatttatt gcgcccca
//
LOCUS       DAOQJE010000063         6679 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_6386_length_6679_cov_3.716636, whole genome shotgun sequence.
ACCESSION   DAOQJE010000063 DAOQJE010000000
VERSION     DAOQJE010000063.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 6679)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6679)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6679
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6386_length_6679_cov_3.716636"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..835)
                     /locus_tag="PLT08_02675"
     CDS             complement(<1..835)
                     /locus_tag="PLT08_02675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE03397.1"
                     /translation="MPDEIVLRFSVRDDGSPVIERINQKVKQTTKETQALAPGIENAR
                     RSVMDFASENAGLIAVLTGTALALKKVYDTAREGAEIEYTRVKFERLAESIGTTSDAL
                     LGELRTATRGTLSDMEAMALTADLLALGLAKNSDEAVRLAKVQSGLGMDMNQLVLTLT
                     NQTTMRFDALGVSVDGFDEKVKQLKATGMSTNEAFKEAFLQQAEEQLNKVGNAADEDI
                     GAFKRLEAQMKNNRDTAKAMSAEALAPLVRYFADSTEKINENKEILRQLNEELYKEYS
                     AH"
     gene            complement(828..1031)
                     /locus_tag="PLT08_02680"
     CDS             complement(828..1031)
                     /locus_tag="PLT08_02680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE03398.1"
                     /translation="MERWGAGSVFEGAIPAKLLTRMSVLSNIYNAFLSYKAGSSNFAK
                     WAEMNPHYFAIVAEIREMRKQYA"
     gene            complement(1103..1528)
                     /locus_tag="PLT08_02685"
     CDS             complement(1103..1528)
                     /locus_tag="PLT08_02685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE03399.1"
                     /translation="MKYKIQDFTLELIDEPVQKHVVKWEKTSRKLRNEDAKPELSKAI
                     DAIKRLSITESDTEKFVSAYRTIAAALEKAFDVLNNNEVLTVSANHGVIVKAAMESGW
                     IISPEMTADEIDELPAWKVTWMAEKVGELYLAVTQVPKN"
     gene            complement(1528..1926)
                     /locus_tag="PLT08_02690"
     CDS             complement(1528..1926)
                     /locus_tag="PLT08_02690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE03400.1"
                     /translation="MTKYDGKDMVFKNGAFSFPSGALVSVDWQEQRDELDVTGASQED
                     KEFLPSERSGQVTVNAWDDAANTIFAANETSDDSATLEFYPQGNTTGKPKISATGFIT
                     SRSRPVAHNQAAAITITYRITGATTYSTVA"
     gene            complement(1930..2109)
                     /locus_tag="PLT08_02695"
     CDS             complement(1930..2109)
                     /locus_tag="PLT08_02695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE03401.1"
                     /translation="MAEKMYVVAKGAILTSRADGKDYFEGQEIDLSHLSPEEIKVLKD
                     AGKITEKGTAQKPKE"
     gene            complement(2118..2579)
                     /locus_tag="PLT08_02700"
     CDS             complement(2118..2579)
                     /locus_tag="PLT08_02700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE03402.1"
                     /translation="MPSSDSINRQTWREQLATLIDTALDSTWDVFNYGTANFNGKARN
                     VVIASGDTEYPEMGAGSRDVAAGEAEFDFYVTLFILYADDSQSWTAKNSEDALDLGRK
                     KIADVIRDNYKVTDYWEKLVPNGRSRVGITPDEGGPVYRYEVIPVRASNYI"
     gene            complement(2569..2988)
                     /locus_tag="PLT08_02705"
     CDS             complement(2569..2988)
                     /locus_tag="PLT08_02705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE03403.1"
                     /translation="MPYIPQYTLEGAQEVQERNLRRIANLRPEGAAGEAVRDAAIALH
                     RHAVQITHVGKYVGGGALKNSHRMQVNGLEAMIYIDPSVISPRRGKRKYRPAVYGVYE
                     HERGGEHAFYDRTADEIGGQVKARAIQHITEAVIYAK"
     gene            complement(2988..3314)
                     /locus_tag="PLT08_02710"
     CDS             complement(2988..3314)
                     /locus_tag="PLT08_02710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE03404.1"
                     /translation="MTDSSFAVMTTVSYTRERATLTGGFSGSGSPTLYGTPVAPVDAE
                     TQLRLQLNTPVTLWEVHMQGDPDLKAGDKLVNGSVKYNIKTKEPYVWLPTNDTRVRLI
                     LEDIRN"
     gene            complement(3307..4131)
                     /locus_tag="PLT08_02715"
     CDS             complement(3307..4131)
                     /locus_tag="PLT08_02715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE03405.1"
                     /translation="MYDHNALINGKFLHNLNGWTPSAGVTYSAGDGDDNYGVAVLPVG
                     GSIWQEFSVDEEALQSLHISVKAVGASLSGSQATLVIKDNYDNTVITQNLSGTADTWT
                     ENLYSVGISEGTTYRITITNNSAAGDIKIDDVWIWFIPITRAQIATAVHAKLGRLATE
                     RSLSTTPSGTLTEGSYTYAIDAGLRTIGAIDEEVGLPSVRFVESEQVQMLIGEVRREM
                     LEFLQTEYAVEVDTSTGPYSQQLSQKASLIGDIIGSGKSGGKGGSGAIIQKRISHD"
     gene            complement(4131..4475)
                     /locus_tag="PLT08_02720"
     CDS             complement(4131..4475)
                     /locus_tag="PLT08_02720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE03406.1"
                     /translation="MSEKNYLIVNPKGVMHVVSEAHARERLKLVGWRLASPEEKQAYF
                     DAKGNQRFDKPLAAPFSPAPLVDEALTEAELMPTKKAAKPELGTNAEAVAGDAKPKTK
                     KAAKTDEAPAGE"
     gene            complement(4491..4874)
                     /locus_tag="PLT08_02725"
     CDS             complement(4491..4874)
                     /locus_tag="PLT08_02725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE03407.1"
                     /translation="MSRFDNTRNLKIKPVFTIGAENTNAINVAIQLLNRDNGNELAER
                     VGLQWYLSSDANGDAISAAPSGGIAIGTDGLLIEHTNNIAGKIISEADGDIDVTLTES
                     TAKSFYLILIAPDGKLYPSGAITFA"
     gene            complement(4924..5820)
                     /locus_tag="PLT08_02730"
     CDS             complement(4924..5820)
                     /locus_tag="PLT08_02730"
                     /inference="COORDINATES: protein motif:HMM:NF023081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P22 phage major capsid protein family protein"
                     /protein_id="HNE03408.1"
                     /translation="MANITTSQVADSTPTIVAAQALGYLKANTVLARLVNRDYENEVA
                     QYGNVVKIPYGGSLSVNDKSGGSVVTLQQPDDAVYTLTLNKHKEVSFLIEDLAKAFAR
                     PDWFSVYAADAMAVLAEQIDADIAALYSGLSQTIDATAGLSEGTFREARRLLNSAKAP
                     LENRVAVLHEDAEYEMLGIEKVVNRDYAESLGSKAAGAFTGRFMGFDVFLDQKIVATG
                     GQCKNLFFHRNAIVMANRPLPTAPAGMGAVQRVMNEDGIAIRVTMSYDHDYLGAKFTL
                     DTLYGVAEMRDSHGVAVSTDEI"
     gene            complement(5889..6413)
                     /locus_tag="PLT08_02735"
     CDS             complement(5889..6413)
                     /locus_tag="PLT08_02735"
                     /inference="COORDINATES: protein motif:HMM:NF025623.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4355 domain-containing protein"
                     /protein_id="HNE03409.1"
                     /translation="MGQTQTETSATQTEQTAQTQTAANQPTTQPAQTPEPVPYERFKE
                     VNEKYKALETQLKDIQDKQKADEEKRLKEQGDYKKLYEDLQAQLSTEKVTNMKLKLAS
                     SKGLPVDLVNRIQGATEEEIGNDIESLLAFMKKETPQGVTPPPRGGTSTNFDYSTATP
                     EQVRKHLEDQKKNQ"
     gene            complement(6425..6607)
                     /locus_tag="PLT08_02740"
     CDS             complement(6425..6607)
                     /locus_tag="PLT08_02740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE03410.1"
                     /translation="METLTMAFQMVYPRVACALNTDGTALTVYDMPLAAGDLKAVIAQ
                     GGNAKSGSVIVFLLEE"
BASE COUNT         1347 a         1817 c         1763 g         1752 t
ORIGIN      
        1 tatgcgccga atattccttg tatagttcct cgtttagttg acgtagaatt tctttattct
       61 cgttgatctt ctcggtcgag tcggcaaagt accgcaccaa cggcgcgagg gcttcggcgc
      121 tcatggcttt tgccgtgtcg cggttgtttt tcatctgcgc ttcaagtcgc ttgaaagcgc
      181 cgatgtcttc atctgccgcg ttgccaactt tattaagctg ttcttcggct tgctgtaaaa
      241 aggcttcctt gaaagcctca ttagtactca tgccagtagc tttgagttgc tttaccttct
      301 catcgaagcc atccaccgaa acgcccagcg cgtcaaaacg catggtcgtt tggttggtca
      361 aggtaagtac aagctggttc atatccatcc ccaagccaga ttgaaccttt gccagccgta
      421 ccgcttcgtc cgagttcttt gccagcccca gcgccaacag atcagccgtc agcgccatag
      481 cttccatgtc agagagcgtc ccgcgtgttg cggtccgtag ttccccgagt agcgcgtctg
      541 aggttgtgcc aatcgactcc gccagccgtt caaacttgac gcgggtgtat tcaatctctg
      601 ccccctcacg ggcagtgtcg tacaccttct taagtgctaa ggcagttccc gtcaagaccg
      661 caatcaagcc cgcgttttca gaggcaaaat ccatcacaga gcgacgcgcg ttttcaatgc
      721 caggcgcaag agcttgtgtc tctttggtgg tttgctttac cttttggtta atgcgttcaa
      781 tgacggggct accgtcatca cgaacagaga accgtaatac aatctcatca ggcatattgt
      841 tttcgcatct cgcggatttc tgccactatt gcaaaataat gcgggttcat ttctgcccat
      901 ttcgcaaagt tggaagagcc cgccttataa ctcagaaagg cgttgtaaat attggatagc
      961 acgctcatgc gtgtgagtag ctttgcgggg atagctccct cgaacacaga gccagccccc
     1021 cagcgttcca cgcgccacgc caaaaccaac tcgggcggcg ggggcgcgtc tttgtcttcc
     1081 gcgcactccg ccgccgccaa aattagtttt ttgggacttg ggtcacagcg aggtacaact
     1141 cgccaacttt ctccgccatc caagtgacct tccaagcggg tagctcgtcg atttcgtcag
     1201 ctgtcatctc gggcgagatg atccagcccg actccatagc agcctttaca atcacgccat
     1261 gattggcaga gacggtcaaa acctcgttgt tgttgagcac gtcaaaggct ttttctagcg
     1321 cggcggcaat cgtgcggtag gcagagacaa acttttcagt gtcgctctct gtgatgctca
     1381 agcgcttgat tgcgtcaatc gccttactca attccggctt ggcgtcctcg ttgcgaagtt
     1441 tgcgcgaggt cttttcccat ttaacgacgt gtttctgcac cggctcgtcg atcaactcaa
     1501 gcgtaaaatc ttgaattttg tacttcatta ggcaaccgtc gagtaggtgg tcgcgccggt
     1561 gatgcgatac gtgatcgtaa tggcggcggc ttggttgtgg gctacagggc gcgagcgcga
     1621 ggtgataaag cccgtcgcgc taatcttggg tttgccagtg gtgttccctt gcggataaaa
     1681 ctcaagggtt gcagagtcgt cgctggtttc attggcggca aagatggtgt ttgccgcgtc
     1741 atcccaagcg ttgaccgtca cctgccccga gcgttcagag ggcaaaaatt ccttgtcttc
     1801 ttgagacgcg ccggtcacgt ccaactcgtc gcgttgttct tgccaatcca cagacacaag
     1861 cgcaccgctg gggaatgaaa atgcgccgtt cttgaaaacc atgtccttgc catcgtattt
     1921 agtcatgctt tactccttcg gcttttgtgc cgtgcctttt tctgtgatct tgcccgcgtc
     1981 tttcaatacc ttgatttctt cgggcgataa gtgagaaagg tcaatttctt gaccttcaaa
     2041 ataatctttc ccatcggcgc gacttgtcaa aattgcacct ttggcgacaa catacatttt
     2101 ttcagccatg ttgctcctta tatgtaatta cttgcgcgaa cggggataac ttcgtaccga
     2161 tacacaggtc cgccctcgtc aggggtaatc cccacgcgag agcgtccatt cggcactagc
     2221 ttttcccaat aatccgtgac cttgtaattg tctcgaatta cgtccgcaat cttttttcgc
     2281 ccgaggtcga gcgcgtcttc cgagtttttc gccgtccagc tttgcgagtc gtcggcgtac
     2341 aaaataaaca gggtgacgta aaaatcaaac tctgcctccc ctgctgcaac gtcgcggctg
     2401 cctgcgccca tctcagggta ttcggtgtca cccgaggcaa ttactacgtt tcgcgctttg
     2461 ccgttgaaat tagcggtgcc gtaattgaat acatcccatg tgctatcaag ggcggtatca
     2521 atcaacgtgg ctagttgctc gcgccatgtc tggcggttaa tcgaatcact acttggcata
     2581 aatcaccgct tcggtaatgt gttgaatagc tcgcgccttg acctgtccgc cgatttcgtc
     2641 cgctgtgcgg tcgtagaacg catgttcacc gccgcgctca tgctcataca cgccatacac
     2701 tgcagggcgg tatttgcgct tgccgcgtcg cggagagata acagaggggt cgatgtagat
     2761 catcgcctca agcccgttga cctgcatcct gtgcgagttc ttgagcgcac cgccgccgac
     2821 gtatttgccg acgtgcgtaa tttgcactgc gtgacgatgc aacgcaattg ccgcgtcacg
     2881 taccgcctcc cccgccgccc cttctgggcg taggttggca attcggcgca agtttcgctc
     2941 ttgcacttcc tgagcgcctt cgagggtgta ttgtgggatg tacggcatta gttgcgaata
     3001 tcctcaagga ttaagcgcac gcgggtatcg ttggtcggga gccacacata cggctctttg
     3061 gtcttgatgt tgtatttcac cgagccattc actaacttgt cacccgcttt gagatcgggg
     3121 tcgccctgca tgtggacttc ccacagcgtg acaggcgtat tgagttgcaa gcgtagttgt
     3181 gtttcagcgt ccaccggcgc aacgggcgtc ccgtaaaggg tcggcgaacc agagccagaa
     3241 aaaccgcccg tcagggtagc gcgttcccgc gtgtacgaga cggtagtcat gacggcgaat
     3301 gagctatcag tcatggctta tccgcttctg aataatcgcg ccgcttccgc ctttgccgcc
     3361 gctcttgcca ctgccgataa tgtcaccaat cagagaggct ttctgcgaaa gttgctggct
     3421 gtacggtcca gtagaggtat ccacctctac cgcgtattcg gtctgcaaaa actcaagcat
     3481 ctcgcgccgc acttcgccga ttaacatctg tacttgttct gactccacaa agcgtacaga
     3541 cggcaaaccg acttcctcgt cgatggctcc aatcgtgcgt aatccggcgt caatggcgta
     3601 ggtgtaagag ccctctgtca atgtaccgct tggggttgta gagagactgc gctcggtggc
     3661 aagtcgtcca agtttggcat gtactgccgt tgcaatctga gcgcgagtaa tcgggataaa
     3721 ccaaatccac acgtcatcaa ttttgatgtc gcctgccgcc gagttgtttg tgatcgtaat
     3781 tcggtaggtt gtgccctcag agatacccac gctgtacagg ttttccgtcc aggtatccgc
     3841 cgtcccactt aggttctgcg taatcacagt gttgtcgtag ttgtctttga taaccaacgt
     3901 cgcctgtgac ccgctcaaac ttgcgccgac ggctttaacg gaaatatgca acgactgcaa
     3961 cgcctcctca tctacggaaa actcttgcca gatgctaccg ccaacgggca agacggcgac
     4021 gccatagtta tcgtcaccgt ctcccgctga ataggtcaca cccgcagagg gtgtccatcc
     4081 gttgagatta tgcagaaact ttccgttgat gagcgcgtta tggtcgtaca ttattcacct
     4141 gcaggggctt cgtcggtctt tgcggctttc ttggtctttg gctttgcatc gccagcaacc
     4201 gcctcagcgt ttgtgcctag ctccggcttt gcggctttct tggtcggcat aagttccgcc
     4261 tcagtcaagg cttcgtccac aagcggagca gggctaaacg gcgcggcgag tggcttatca
     4321 aaacgctgat tgccctttgc gtcaaagtag gcttgctttt cttcggggct tgccaaacgc
     4381 cagccgacca gcttcaaacg ctcgcgggca tgtgcctcag agacgacgtg catcacccct
     4441 ttggggttga caatcagata gttcttttcg ctcacggaaa atcctttcta ctaagcgaac
     4501 gtgatagcac cggatggata cagtttgcca tcgggtgcaa tgaggatcag gtaaaacgac
     4561 ttcgcggtgc tctcggtgag agtcacgtca atgtcgccgt ctgcctcaga gatgattttt
     4621 ccggcgatgt tgttggtatg ctcaatcaac aagccgtcag tgccgatggc aatcccgccg
     4681 ctgggagcgg cagagatggc gtcaccattg gcatcactgg acaagtacca ctgcagacca
     4741 acgcgctcgg cgagttcgtt gccgttatcg cggttaagca attggatagc aacattgatg
     4801 gcattggtat tttcagcgcc aatcgtaaag actggcttga ttttcaaatt acgggtgtta
     4861 tcaaagcgag acatagggag aatcctttac ggggcagggt cgcgccctgc cccgttatgg
     4921 ttcttagatt tcgtcggtgc tcacagcgac gccgtggctg tcgcgcattt cagcaacgcc
     4981 gtagagggtg tcgagggtga actttgcgcc gagatagtcg tgatcgtagg acatggtcac
     5041 gcggatggcg atcccgtcct cgttcatcac gcgctggacc gcacccatac cagcgggggc
     5101 ggtaggaagc gggcggtttg ccatcacgat ggcgttgcgg tggaagaaca agttcttgca
     5161 ctgtccgccg gtggcgacga tcttctggtc aaggaaaacg tcgaagccca tgaagcgacc
     5221 agtgaacgca ccggcagctt tcgagccgag ggattcggca taatcgcggt tgaccacttt
     5281 ttcgatacca agcatttcat actcggcgtc ttcgtgcaac acagccacgc ggttctcaag
     5341 cggagccttg gcagagttca gcaagcggcg ggcttcgcgg aaagtgcctt cgctcaagcc
     5401 agcggttgcg tcgatggtct gagacaagcc agaatacagc gcggcaatgt cggcgtcgat
     5461 ctgctccgcc aaaaccgcca tcgcatcggc agcatacacg ctgaaccaat cagggcgagc
     5521 aaaggctttt gcaaggtctt cgatgaggaa agagacttcc ttgtgcttgt tcagcgtcaa
     5581 ggtataaacg gcatcatccg gctgttgcag ggtcacaaca gagccgccgc tcttgtcgtt
     5641 gacagagagc gagccgccgt aagggatttt gaccacgttg ccatactgcg caacttcgtt
     5701 ctcgtagtca cggttgacaa gacgggcaag cacggtattt gctttcaggt agccgagtgc
     5761 ttgagcagct acgatggtcg gggtggaatc cgcgacctga gaggtagtga tattagccat
     5821 ttcaaaggtt ccttataggt tgatagggtt ttgaaaggcg gtacggttta cgtcgtcgag
     5881 actgtgcctt attggttttt cttttgatct tccagatgct tgcggacttg ttccggcgtc
     5941 gcggtggagt aatcaaaatt cgtgctggtt ccgccacgag gcgggggtgt taccccttgc
     6001 ggggtttctt tcttcatgaa ggcgagtagg ctttcgatgt cgtttccgat ttcttcctcg
     6061 gttgcaccct gtatcctgtt cacaagatca acgggtaaac cttttgagga agccagtttc
     6121 agcttcatgt tggtcacttt ttcggtgctt aattgcgctt gcaggtcttc atacaatttc
     6181 ttgtaatcgc cctgctcttt cagccgtttt tcttcgtcgg ctttttgctt gtcctgaata
     6241 tccttgagtt gtgtctcaag cgctttgtac ttctcgttca cttccttgaa ccgctcataa
     6301 gggacgggtt caggggtttg cgctggttgc gtggtcggct gattcgccgc tgtttgtgtt
     6361 tgcgcggtct gttcagtttg agttgcagat gtttcggttt gggtttgtcc catgcaatac
     6421 ctttctattc ctcaagcaaa aatacgatga cgctaccgct tttggcattg ccaccttgag
     6481 caataactgc ttttaggtcg cccgccgcaa ggggcatgtc gtacacggta agcgcggtgc
     6541 cgtctgtgtt caacgcacag gcaacgcgcg gataaaccat ctggaacgcc atcgtcaaag
     6601 tttccatcca caagctacac agcgtacacg cgcccaaaga cccgttctgc cgttacagtt
     6661 gcgtttccag atgcgtccg
//
LOCUS       DAOQJE010000064         6665 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_6401_length_6665_cov_5.830560, whole genome shotgun sequence.
ACCESSION   DAOQJE010000064 DAOQJE010000000
VERSION     DAOQJE010000064.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 6665)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6665)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6665
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6401_length_6665_cov_5.830560"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..259
                     /locus_tag="PLT08_02745"
     CDS             <1..259
                     /locus_tag="PLT08_02745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE03411.1"
                     /translation="PPVIHRVEPSETGLRIYASDDVRVANVSITIFGEDEGVLEKGDA
                     VQVGDSEWWEYAAQHAGRVLVEARDLAGNVARMEGGLGIRG"
     gene            complement(450..668)
                     /locus_tag="PLT08_02750"
     CDS             complement(450..668)
                     /locus_tag="PLT08_02750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE03412.1"
                     /translation="MNLKEIIQAVENLSPGELSHFRDWFKRHETPANSGKLDSQPIEI
                     TLKRLQGSLKGSGALQTLMEERRKESLL"
     gene            complement(696..1463)
                     /locus_tag="PLT08_02755"
     CDS             complement(696..1463)
                     /locus_tag="PLT08_02755"
                     /inference="COORDINATES: protein motif:HMM:NF024783.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HNE03413.1"
                     /translation="MALNKWTREELILAFNHYFKIPFGKIDDRTPEIIALAKIIGRTP
                     SAVALKLANFAHFDPTLQERGIKGASHGSKLDAVIWAEFYGDIEKLSFESEVLLAKKT
                     GKQVEEVAGIYVLDLPKEGREREALVKTRVNQSFFRKTILAAYSVRCCITGLSIPALL
                     TASHIVPWSEDAENRMNPRNGLCLNALHDRAFDKGWITVTPDLKVKVSPKIQRITADK
                     AASELILKYDGVSIKEPKRFLPDKKFLEYHNDVIFMK"
     gene            1723..2136
                     /locus_tag="PLT08_02760"
     CDS             1723..2136
                     /locus_tag="PLT08_02760"
                     /inference="COORDINATES: protein motif:HMM:NF041216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CU044_2847 family protein"
                     /protein_id="HNE03414.1"
                     /translation="MATQIRELHLEDGEKIFVAVEVIEDINTSLNLKGINDLPPGAEP
                     TGVIDSGLMTAHLLKAYIKSMAKSTKEALQEASPDEFSVELSIGFVGKTNPIPVIAGA
                     DIQGGIKITATWKKEEANQNLSKKKPNNSKEHNSK"
     gene            2133..5876
                     /locus_tag="PLT08_02765"
     CDS             2133..5876
                     /locus_tag="PLT08_02765"
                     /inference="COORDINATES: protein motif:HMM:NF013124.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043531 - ADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NB-ARC domain-containing protein"
                     /protein_id="HNE03415.1"
                     /translation="MNWQGSIVRVWDGLPSNGGIFIGTAFFIDSRTLLTAKHVVENIQ
                     HNIYLDGTPGGGKEKIEPENITLCERDIAVLKSSRSFTSVLNLNVLNTEIKVGQKLTL
                     AGYIDEIQSLHTLTSEVSGHVSNLHTWRTQGNISRGMSGGPVIDINDNVVGLIQARDT
                     DKNLCYLIPSEVITSCLEKTGLLTSIKKAPLYNVPKLPPHFINRSEHIEKIKELLLKE
                     SGRNVGITGISHTIGLQGMGGIGKSILAASIAYDREIRNAFYDGIIWVTIGQNPDIIN
                     LQSIILEQLDASGIIFDDPEHGRTLLSNHLRDKKILLILDDVWKINDVYYFDISSIES
                     KVVITSRHSQVLRGVSAEEYKIDVLPPNQAKQLLRIQSGWEKDNMPEEAELILNECGA
                     LPLAISMIGSMLRNRPQNRWQLILRHLRNAELQSIRHQFKDYPYPDLFIALHVSLEAQ
                     PAIIQNIYLSLAIFPQDIPIPESVLEIYWNKEDLQHYDVLEIIDELLDASLIFRYNEN
                     NLILHDLQNDYIRLQCSDIQTYHRKIVAGYEKNYPLGWSTIPYETPWYFHNRIVYHLI
                     QMGDLERTKAIIEKILSEKNFLTWKDILICSQIINKTPKELARQAIKTNLQPSTLIEC
                     IKLLKNEAKDEALQLLRQDNQHQYVITTCLNLLGAEAKDEARRLLKQDNLHPYIITAC
                     LKLLGAEAKDEARRLLKQDDQNQQVITSCLNLLGVEAKDEARRLLKQDNQHTYIITTC
                     LKLLGVEAKDEARRLLTQGDKNYQVITTCLKLLGAEAKDEARRLLKQDDQHTIIITAC
                     LNLLGVEAKDEARRLLKQDNQYKEVITTCLKLLGAEAKDEARRLLKQDDQFQDVITTC
                     LNLLGAEAKDEARRLLKQDNQYKEVITTCLNLLGVEAKDEARRLLKQDDKNQQVITTC
                     LNLLGVEAKDEARRLLKQDDQSQEVITACLNLLGAEAKDEARRLLKQGDKNNQVITTC
                     LNLLGVEAKDEARRLLKQDDQFQDVITTCLKLLGTEANDEARRLLKQDNQYQEVIMAC
                     LNLLGTEAKDEARRLLKQDDQFQDVITECLSILGDEAKEDAKRLLKQGNQNPSVIISC
                     LDILGIESSGFVTKELISGNWDNLTGPLKIKILSIPIKEKIREQKAMEILTKYYLYQR
                     SLVKTALDVFNKDYQKVKKTCLEILQKWESDYDYYQKKGSPKYSGHIIKAMRHPELRN
                     QAKLVAIEVLNKHEEKPGYLDEELPEFCQYFVEGVEFDWTDEM"
     gene            complement(6132..>6665)
                     /locus_tag="PLT08_02770"
     CDS             complement(6132..>6665)
                     /locus_tag="PLT08_02770"
                     /inference="COORDINATES: protein motif:HMM:NF012363.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylesterase family protein"
                     /protein_id="HNE03416.1"
                     /translation="LQELYDLYASDPQRIWTAEGDIILAMGDDYAFRVQTLDVAAAQA
                     QVARTYVYRVSYPVNLPDQPCQNGRSPHGGELPFVFGRVNDGTGFDFIGLPRDAQDDL
                     ARNRLQGQMIRAWTNFARTGDPNGTDLPQWPRFEAETQPVFIFDADPRVEQAPFFAEY
                     QAMSEFMQVFNVFDALK"
BASE COUNT         2108 a         1359 c         1514 g         1684 t
ORIGIN      
        1 cccgccggtc attcaccgtg tggaaccgag tgaaaccggg ctgcgcattt acgcgagcga
       61 cgatgtgagg gtggcaaacg tatccatcac tatttttggc gaggatgaag gggttttgga
      121 aaaaggggat gccgtgcagg tgggcgactc tgaatggtgg gagtatgctg cccagcacgc
      181 aggcagggtt ttggtagagg cgcgcgatct ggccgggaat gtggcgagga tggagggcgg
      241 gttagggatt aggggttagg ggttaggggt tcgagggtag gtattcgaga ttgggggttc
      301 gatattcgat actcgatatt cgagagtaga gagtagatat ttgagattgg agataggggg
      361 gctgggttta gggggggccg ggtgtgtttt gtggggtcgg tgggtgcgaa ataaaaactc
      421 cgagcgtatt tagtgcctgg agtgtgcgat cataaaaggc tttctttacg acgttcttcc
      481 attaaagtct gcaaggctcc cgaccctttc aatgagcctt gtagccgttt gagggttatt
      541 tcaattggct gtgaatccaa tttaccagaa ttagcagggg tctcgtgtct cttgaaccaa
      601 tcacgaaaat gagagagttc ccctggcgac agattttcta cagcctgtat tatttctttg
      661 agattcatat ccaactccgt acagagaaag taacattact tcataaagat aacgtcgtta
      721 tgatactcca ggaacttttt atcgggtaaa aatctttttg gctctttgat cgagacacca
      781 tcatacttca agatcagttc agaagccgct ttatcagccg taattctctg tatcttaggc
      841 gatactttga ccttaagatc aggggtaacg gttatccatc ctttatcaaa cgctcggtcg
      901 tggagcgcat tcaggcaaag gccatttctt gggttcatac gattttcagc atcctcagac
      961 cacggaacaa tatggctggc agttaacagg gcaggaatac ttagacctgt gatacaacac
     1021 ctaacactat acgcggccaa aatcgttttt cggaagaaac tttggttcac ccgagttttc
     1081 actaaggctt cacgttctct accctcttta ggcaagtcaa gaacataaat cccagccacc
     1141 tcttcgactt gtttgcctgt ttttttcgct agcaagacct cactttcaaa acttagtttc
     1201 tctatatctc cataaaactc ggcccatata acagcgtcta atttactgcc gtgcgaagca
     1261 cccttgatcc ctctttcttg caaggttggg tcaaaatgcg caaaattagc cagtttcaac
     1321 gccacagcag agggggtacg tccaatgatc ttggcaagag caatgatctc aggcgtacga
     1381 tcatcgatct tcccgaacgg gatcttgaaa taatggttga aagcaaggat cagttcttct
     1441 ctggtccatt tgtttagagc catatttacc ctctttcaaa ataaggctaa aaaatatacg
     1501 aattgacctg ctctgagagc cgccaagcgg acttgaaggt ccgtgtccat gaagggattt
     1561 tactctatac agccaaagac tatcgcggga gattttggaa ggggaattcc ctctcggccc
     1621 gtttagtttg caacttgtaa tgtaactaaa gaataaatgt ccgctataat aaccctcgac
     1681 cttcattgaa aaatatatca tttaagtctc agaggaggca aaatggctac tcaaattcga
     1741 gaattacatt tagaagatgg ggaaaagatt tttgtagcag ttgaggtaat tgaagatata
     1801 aatacctcat tgaatctcaa agggataaat gatctaccac ccggtgctga gccaacaggg
     1861 gtaattgaca gcggtttaat gacagcccat cttcttaagg catacatcaa aagcatggca
     1921 aaatctacaa aagaagcgct acaagaggca agcccagacg aattttctgt agagttaagt
     1981 attggctttg ttggaaaaac aaacccaatt ccagttattg ccggtgcaga tattcagggc
     2041 ggaatcaaga taactgctac ctggaaaaaa gaagaagcca atcaaaatct gtcaaagaaa
     2101 aaaccaaaca actcgaagga acataatagt aaatgaactg gcaggggtca atcgttcgtg
     2161 tctgggatgg gctaccttcc aatggcggaa tttttattgg aactgccttt tttatcgatt
     2221 ctaggacatt attaacagct aaacatgttg tagaaaatat tcaacataat atttacctag
     2281 atggaacacc aggtggaggc aaagaaaaaa tcgagccgga aaacatcaca ctatgtgagc
     2341 gcgacattgc tgttttaaaa tctagtcgtt cattcaccag tgtacttaat ttaaatgtat
     2401 tgaacacaga gattaaagtt ggccaaaaat taacactcgc aggctatata gacgaaattc
     2461 aatctttaca cacactaaca tctgaagtgt cagggcatgt tagtaattta cacacttggc
     2521 ggacacaagg caacatcagt cgcggaatga gcggcggacc ggtaattgat ataaatgata
     2581 atgttgtagg ccttattcaa gctcgtgata ctgataaaaa tttgtgctat ctgataccat
     2641 ctgaagttat tacctcatgc cttgaaaaaa caggattact gacatcaata aaaaaagcac
     2701 ctctatacaa tgtacccaaa cttcctccgc attttataaa tcgaagtgaa catatcgaaa
     2761 aaataaaaga gctgctacta aaagagtctg gaaggaacgt tggaattacg ggtatttctc
     2821 atactatagg tttacaaggg atgggcggta ttggtaaatc tattttagct gcatctattg
     2881 cttacgatag agaaattcgc aatgcttttt acgacggcat tatctgggta actattggtc
     2941 aaaacccaga tattatcaat ttgcaaagta tcattctcga acaactagac gcatctggaa
     3001 ttatttttga tgaccctgaa cacggaagga ccttattaag taatcatcta agagataaaa
     3061 agattctact catattagat gatgtttgga aaattaacga tgtttattat tttgatattt
     3121 cctctatcga atcgaaagtg gtgattactt cccggcattc acaagtctta agaggagtta
     3181 gcgctgaaga gtataaaatc gatgtgctgc caccaaacca agcaaaacaa ttactgcgca
     3241 ttcaatctgg gtgggaaaaa gacaatatgc cagaggaagc agaattaatt ttaaacgaat
     3301 gcggggcatt gccgctcgct atcagcatga tcggatctat gttaagaaac aggcctcaaa
     3361 atcggtggca actaattttg aggcatcttc gcaatgcaga actacaaagt atccgtcatc
     3421 aatttaaaga ttatccatat ccagatttat ttattgcatt acatgtcagc ttagaagcac
     3481 aaccagccat aatacaaaat atctatctta gccttgctat attcccccaa gatataccga
     3541 ttcctgagtc agtgctcgaa atttattgga acaaagagga tttacaacat tatgatgtat
     3601 tagaaatcat agatgagctt ttagatgcct cattaatttt tcgttacaat gaaaacaatc
     3661 ttatactaca tgatcttcaa aacgattaca ttcgtttgca atgctctgac atacaaacct
     3721 atcatagaaa aatagtcgct ggttacgaaa aaaactaccc tcttggttgg tccacaatac
     3781 cttatgaaac tccatggtat tttcataaca ggattgtata tcatctaata cagatgggag
     3841 acttagaaag aactaaggcg attattgaaa aaatattgag cgaaaagaac tttctaactt
     3901 ggaaagatat tttgatttgc agtcaaatca ttaataaaac acctaaggaa ttggctcggc
     3961 aagcgattaa aacaaatctg caaccatcaa ctctaattga atgcatcaaa ctcctaaaaa
     4021 atgaagcaaa agacgaagcg ctacaactac tcaggcaaga taatcaacac cagtatgtca
     4081 tcacgacttg cttgaatctg cttggggcag aagcgaaaga cgaagcgcga cggctgctta
     4141 aacaagataa tctacaccct tatatcatca cggcttgctt gaaactgctt ggggcagaag
     4201 cgaaagacga agcgcggcga ctgctcaaac aagatgatca aaaccaacaa gtcatcacaa
     4261 gttgtttgaa tctgcttggg gtagaagcga aagacgaagc gcgacgacta ctcaaacaag
     4321 ataatcaaca cacttatatc atcacaactt gcttgaaact gcttggggta gaggcgaaag
     4381 acgaagcgcg acgactgctc acacaaggtg ataaaaacta ccaagtcatc acgacttgct
     4441 tgaaactgct tggagcagaa gcgaaagacg aagctcgacg actactcaaa caagatgatc
     4501 aacacactat tatcatcaca gcctgcttga atctgcttgg ggtagaagcg aaagacgaag
     4561 ctcgacgact actcaaacaa gataatcaat ataaagaagt catcacgact tgcttgaaac
     4621 tgcttggggc agaagcgaaa gacgaagctc gacggctgct caaacaagat gatcaatttc
     4681 aagatgtcat cacgacttgc ttgaatctgc ttggagcaga agcgaaagat gaagcgcgac
     4741 ggctgctcaa acaagataat caatataaag aagtcatcac gacttgcttg aatctgcttg
     4801 gggtagaagc gaaagacgaa gcgcgacgac tactcaaaca agatgataaa aatcaacaag
     4861 tcatcacgac ttgcttgaat ctgcttgggg tagaagcgaa ggacgaagcg cgacgattac
     4921 tcaaacaaga tgatcaatct caagaagtca ttacggcttg cttgaatctg cttggggcag
     4981 aagcgaaaga cgaagctcga cggctgctca aacaaggtga taaaaacaac caagtcatta
     5041 cgacttgctt gaatctgctt ggagtagaag cgaaggacga agcgcgacga ttactcaaac
     5101 aagatgatca atttcaagat gtcatcacaa cttgcttaaa actacttggg acagaagcga
     5161 atgacgaagc gcgacgattg ctcaaacaag ataatcaata tcaagaagtt attatggctt
     5221 gcttgaattt gcttgggaca gaagcgaaag acgaagcgcg acgactgctc aaacaagatg
     5281 atcaatttca agatgtcatc acagagtgct taagtattct aggcgatgaa gctaaagagg
     5341 acgcaaaaag attactaaaa cagggaaatc aaaatccttc ggtgatcata tcgtgcttag
     5401 atatattagg tatagagagc tcagggtttg tcacaaaaga actaattagt ggaaactggg
     5461 ataatctaac tgggcctttg aaaattaaaa ttttgagtat tcctattaaa gagaaaatac
     5521 gagaacaaaa ggcgatggaa attcttacaa agtactacct atatcaacgc tctttggtca
     5581 agacggcctt agatgttttc aacaaagatt atcaaaaggt aaaaaagact tgcctcgaga
     5641 tactgcaaaa atgggaaagt gactatgact attaccaaaa aaagggatca cctaaatact
     5701 ctggtcacat aatcaaggca atgcgtcatc cagaattaag aaatcaggcc aagctggttg
     5761 cgattgaagt actcaataaa catgaagaaa agccagggta tcttgatgaa gaactaccag
     5821 aattttgtca atactttgtt gaaggcgtcg aattcgattg gacagatgaa atgtaactta
     5881 ttaatactgt tattggtcat aacaatactg tgcgctctgt aaaaacttct aaccgaactc
     5941 tacattccaa acctacagtc tcgaatacca aactattcaa tttttaattt atctgctaca
     6001 agaaactcag gtaaacgaag cctcacgagt cttctaaagc caactagatt tcgcaccaac
     6061 cttcaacacc acaaagaact ccgggttcat cgcccggagt tctttgtatc ccttccccac
     6121 tgaagagggc cttacttcaa agcatcgaag acattgaaga cctgcatgaa ctcagacatg
     6181 gcttgatatt cggcgaagaa gggcgcctgc tccacgcggg ggtcggcgtc gaagatgaag
     6241 accggctggg tttcggcttc aaagcggggc cactggggca ggtctgtgcc gttggggtcg
     6301 ccggtcctgg cgaagttggt ccaggcgcgg atcatttgtc cctgcaggcg gttgcgggcg
     6361 aggtcatcct gcgcatcgcg gggcaggccg atgaagtcga agccggtgcc gtcgttgacg
     6421 cgtccgaaga cgaagggcag ttccccgccg tgcggtgagc gtccgttctg gcagggctga
     6481 tcgggcaggt tgaccgggta actgacgcga tagacatagg tgcgggcgac ctgggcctgc
     6541 gccgctgcga cgtccagggt ttgcacgcgg aaggcgtagt catcgcccat ggcaaggatg
     6601 atgtcgccct cggcggtcca gatgcgctgg gggtctgagg cgtacaggtc atagagttcc
     6661 tgcag
//
LOCUS       DAOQJE010000065         6597 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_6510_length_6597_cov_5.132223, whole genome shotgun sequence.
ACCESSION   DAOQJE010000065 DAOQJE010000000
VERSION     DAOQJE010000065.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 6597)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6597)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6597
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6510_length_6597_cov_5.132223"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1666
                     /locus_tag="PLT08_02775"
     CDS             <1..1666
                     /locus_tag="PLT08_02775"
                     /inference="COORDINATES: protein motif:HMM:NF020337.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="CotH kinase family protein"
                     /protein_id="HNE03417.1"
                     /translation="TLVNITSVEDVNNQPIKDNLALYENDDPTSVVCMYVTVRKGNSL
                     ENTEYTWEEVNNFDKWTNKGSRAANDVVGQAEVIFQIGDETGPLPGELGYNISVPNGT
                     IQIRGASTSANGNQKSYKIELYDDAGLWRGQSTIALNKHVFDPTRVRNKLSFDLLKGI
                     PGIVTLRTQFVHLYVKDETTSFAGKGFVDYGLFTQVEAVNKAYLRNHQLDPNGQLYKA
                     TSFEFYRYEDQIRLESDPLFDESEFSHRLEIKGNRDHSKLIQMLNDINNYDIPIAQTF
                     EKYFNEDNYFTWLAYNILVGNVDTQNQNFYLYSPQNSEKWYFIPWDFDGDLERQKKEE
                     LDSDPYLSWEVGLHNYWGGVLHNRLLRLEKYRTMLDNKINELSIAFPADRLRTMLNSY
                     RAVTEPFVFSAPDVKHLSRNKYQYNLSFELIPTEIENNKKLYFETQKYPTPFFLGTPE
                     IIDGELRFTWDQSFSFTPQVITYRFLISKDLLFNNIAFQSDLVESTEISTPVFEPGVY
                     YWRVIAINEEGFNRFPFDAYFGQGFSRYDGMKFFMINQDGTLGDAQ"
     gene            1784..2497
                     /locus_tag="PLT08_02780"
     CDS             1784..2497
                     /locus_tag="PLT08_02780"
                     /inference="COORDINATES: protein motif:HMM:NF020916.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate polymerase domain-containing
                     protein"
                     /protein_id="HNE03418.1"
                     /translation="MSEKKKYRHELKYLINYQEYFLIRSRVRNVLPSDSNVDGDGSYT
                     VRSLYFDDYNNSSYYDKYAGILKRAKHRIRIYNYSDQRINYEKKNKYGQYNHKETALI
                     TKEQFYAILSGDFEFLLKSPNSLLSKLYYECRINLLRPRIIVDYEREPYVLAAGDVRL
                     TFDKNIRAGIDGFDIFDKDIPTIETMDPGTLVMEVKYTEFLPNIVRDILASSSALYVE
                     SSKYILCCDKMLHTRGFNT"
     gene            2533..3195
                     /locus_tag="PLT08_02785"
     CDS             2533..3195
                     /locus_tag="PLT08_02785"
                     /inference="COORDINATES: protein motif:HMM:NF027640.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4956 domain-containing protein"
                     /protein_id="HNE03419.1"
                     /translation="MENILNFLQMDFAAASALLAPVVLALASSFGFGIIIYYVYQKSF
                     RGVVYNQSFSVSLAILTILTTMITLAISTNIALSLGMVGALSIVRYRTAIKDPADIVF
                     LFWAIGTGIAVGAKSHYLALVASIVVILMLYTIGRKATNREVYILIIHYIGDDITSEL
                     RQILRGKKFQTKSKTVRKESVELALEIEVKNNNLAFLDAIRDLSTVKDVSLIQYAGEY
                     NN"
     gene            3333..4754
                     /locus_tag="PLT08_02790"
     CDS             3333..4754
                     /locus_tag="PLT08_02790"
                     /inference="COORDINATES: protein motif:HMM:NF027442.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4832 domain-containing protein"
                     /protein_id="HNE03420.1"
                     /translation="MGKYSLRHFIFYGFITIVICLSLVFVLSNLYSELDQKIYYFEPL
                     DIPFANPLMGLAPWANQDNILQPHTLVYADLSWRNFEPQEGLYDFATFEASNQLSRWR
                     LEGKRVVFRFVTDFPGDENHIDIPDWLFEKTGGDGTFYDNEYGRGFIPDYANPNFMYY
                     HHRAIKILGEHYGGDDFFAFIELGSLGHWGEWHTYDMQLFPEEDVRNKYVLDYVEAFP
                     DTHLLMRRPFKIADDLNFGLYNDMTGNSKATTEWLDWIDKGGSFLPQENGGLVSMSNA
                     WELAPIGGEQAPTVKNAEMYASELASTLLLLKQSHTTFIGPGGPYDVPLDSPLQAGID
                     EVLSVIGYRLYISRVETPTVVRFHHSIKVKIEFSNSGIAPFYYPWKVYLYLFDEEGNI
                     AKKDLLPIDVRRILPGQTFSISYLLPSKNLDNGTYTIGIAIIDPLTNAPGIRLANNNE
                     RTDLIQQIGSFQVKWIDNFFVTK"
     gene            complement(4819..4891)
                     /locus_tag="PLT08_02795"
     tRNA            complement(4819..4891)
                     /locus_tag="PLT08_02795"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4856..4858),aa:Arg,seq:tcg)
     gene            4969..5691
                     /locus_tag="PLT08_02800"
     CDS             4969..5691
                     /locus_tag="PLT08_02800"
                     /inference="COORDINATES: protein motif:HMM:NF024950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HNE03421.1"
                     /translation="MLSNSLALVTGSAQRLGKAFALTLARMGYSIALHYRGSVTEAEK
                     TTAEIRALGVDCLPIRADLTVPDKIEFLFSMVDEFHLPLKVVVNSAALMPVGNPRDLE
                     LNDWNSALDLNLRAPFLIAQEAAKRMEQGGLIINISDVGAQKPWSRYPSYTVSKAGVE
                     SLTKMLARAFAPSIRVNAIAPGLVLPSDVVTPEKWEQLVKRLPLQRAATLEEITTTLE
                     FLIKNEYITGQTIVVDGGYSLI"
     gene            5722..>6597
                     /locus_tag="PLT08_02805"
     CDS             5722..>6597
                     /locus_tag="PLT08_02805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE03422.1"
                     /translation="MNYKKLLPDILVSALLLASVLYYAFNYINFDIPPFEDAAMIMRY
                     SQHLAGGHGIVWNIGEHPVDGATDFLFMVAAAGLIKLGLTVGQAVRGIGFASHLLTVL
                     LIYWTNRRVHNGNILFSFLSGLYLVVGTGLSYVSAYFGTPFFALAAASTWTLGLILMK
                     EENPRWWLSLAFALSGLVTGLIRPEGVILASLMLLAVIVMRGLKRSVSIIAIFGIVFL
                     TLGGSYFLWRWNYFGYPLPNPFYKKGEGGWSWLTFDASMLNMLRLSLPVVLAFILGFR
                     SNETLKTTLAYMVPIF"
BASE COUNT         1873 a         1174 c         1361 g         2189 t
ORIGIN      
        1 aacacttgtt aacattactt cagttgaaga tgtcaataat cagcccatta aggataattt
       61 ggctttgtac gaaaatgatg acccaacttc tgttgtatgt atgtatgtaa ccgttcgtaa
      121 aggaaattct cttgaaaaca cagagtacac ttgggaagag gtaaacaatt ttgataagtg
      181 gacgaataaa ggctcacgtg cggcaaatga tgtggttgga caagctgaag ttatttttca
      241 aattggagat gagaccggac ccttgcctgg tgagctaggt tataacattt cggttcctaa
      301 tggcactatt caaattcgtg gggcttcaac ttctgcaaat gggaatcaga aatcatataa
      361 aatcgaactt tatgatgacg cagggctatg gcgtgggcaa tctacaattg cacttaataa
      421 gcatgtcttt gatcctacac gcgttagaaa taagttgtcc tttgatcttc taaaaggaat
      481 tcctggtatt gttaccttgc ggacgcagtt tgttcattta tacgttaaag atgaaacgac
      541 ttcgtttgca gggaaaggat ttgtagatta tggcttgttt acacaggtag aagcggtaaa
      601 caaagcttat ctaaggaatc accaattgga tcctaatgga caattgtata aagcgacctc
      661 attcgaattt tatagatacg aagatcaaat tcgtcttgag tcagaccctt tgtttgatga
      721 aagcgagttt tcacatcgtt tggaaatcaa aggaaatcgt gatcactcca aattgatcca
      781 aatgttgaat gatataaata attatgatat tccaatagct caaacctttg aaaaatactt
      841 caatgaagat aattatttca cttggcttgc gtacaatata ttagttggga atgttgatac
      901 acaaaatcaa aatttttatt tgtatagtcc tcaaaacagc gaaaaatggt attttatccc
      961 ttgggatttt gatggcgatt tggaacgaca aaaaaaagaa gaacttgact cagacccata
     1021 cttaagttgg gaagttggac tccataatta ttggggcggg gtattgcaca accgcttgct
     1081 tcgtttggaa aaatatcgaa ccatgctgga taataaaatt aatgaattga gcatagcatt
     1141 ccccgctgat agactaagaa caatgttgaa tagttatcga gccgtaacgg agccttttgt
     1201 tttcagtgcc cctgatgtta aacacttatc tagaaacaag tatcaatata acctctcttt
     1261 tgagcttatt cctactgaga ttgaaaataa caaaaaactg tattttgaaa cccaaaaata
     1321 tcctacacct ttttttctag gtactcctga gataattgat ggcgaactta gatttacttg
     1381 ggatcaatca tttagtttta caccgcaagt cattacctat agattcttaa taagtaaaga
     1441 tttacttttt aataatattg cctttcaatc tgacttagtc gaaagtacag agattagtac
     1501 acctgtgttt gaaccgggag tttattattg gagggttata gcaattaatg aagagggttt
     1561 taatcgtttc ccatttgatg cgtactttgg acaaggtttt agtaggtatg acggcatgaa
     1621 gttttttatg ataaatcaag atgggacttt aggagatgct caataattga ttattgtata
     1681 aattactctt taggggttat cagtaataat tgtgttttac aataaaaaca taaatattac
     1741 cggtatgtga tgactttata tagaaaccac tatttggtga aaaatgtcag aaaagaagaa
     1801 atatcgtcat gaacttaagt acttaataaa ttatcaggaa tattttctga tacgttctcg
     1861 agtaaggaat gtacttccat ctgactcaaa tgtagatggc gatggatctt acacagtccg
     1921 aagtttgtat tttgatgact acaacaatag ctcttattat gataaatatg caggtatttt
     1981 gaagcgcgcc aaacatcgta ttcgaatata caactattct gatcaaagaa taaattatga
     2041 gaaaaaaaac aaatatgggc aatataacca taaagagact gcattaatta ccaaagagca
     2101 attttatgcg attttaagtg gtgattttga gtttttattg aaaagtccga atagcttatt
     2161 aagcaaactc tattatgaat gtaggataaa tttattgcgc cctagaatta ttgtggatta
     2221 tgagcgtgag ccttatgtat tggcagccgg tgatgttcgg cttactttcg ataaaaatat
     2281 tcgtgcaggt attgatggtt ttgatatttt tgataaagat atcccgacta ttgagactat
     2341 ggatcctggt actttagtga tggaagtcaa gtatactgaa tttttgccta atatagtaag
     2401 ggatatttta gcttcatctt ctgcgttgta tgtagagtct tcaaaatata ttctttgttg
     2461 tgataaaatg ttgcacactc ggggttttaa cacctgatat taatactatt tcgtttgtaa
     2521 agaggcataa taatggaaaa tatacttaat tttttacaga tggattttgc cgcggcgtca
     2581 gcattgttgg cgccggtcgt tttagcgttg gcttcttcat ttggttttgg aataatcata
     2641 tattatgttt accaaaagtc atttcgaggt gtggtataca accaatcttt ttctgtttca
     2701 ttggctattt taaccattct gacaacaatg attacactag caattagtac taacattgca
     2761 ctttctttag gtatggtagg cgcacttagt atagtgcgtt atcgaactgc aattaaagat
     2821 cctgctgata ttgtttttct tttttgggct attggaaccg gtattgccgt tggcgctaaa
     2881 tcccactact tggcactagt ggcatctatt gttgttatcc taatgttgta tacgattggg
     2941 agaaaagcta ccaatcgtga agtgtatata ttaattattc attacattgg cgatgatata
     3001 actagtgagc taagacaaat attacgaggg aagaaatttc agacaaaatc taagacggtt
     3061 agaaaagaaa gtgttgagtt agctttagag atagaggtta agaataataa cttggctttt
     3121 ttagatgcaa ttcgagattt gtctacagtc aaagatgttt cgttgattca atatgctggt
     3181 gagtataaca attgaaaacc ttgtacaaag tatcttgtca tttctctaca tgaatcttaa
     3241 agatgagcac tgttgtaact ttgaatgctg atacagagca caaatctatt cacgcatgtg
     3301 aaattcattg tgccttaatg cattgatctt ttatgggcaa atattcatta aggcacttta
     3361 tcttttatgg atttattact attgttattt gcctgagctt ggtttttgtg ttgtctaatc
     3421 tctactctga attagatcaa aagatatact actttgagcc tttggatatt ccttttgcta
     3481 atcctttaat gggcttagct ccttgggcta accaagataa tatacttcaa cctcatacac
     3541 ttgtttatgc tgatttatct tggcggaact ttgagcctca agaaggttta tacgactttg
     3601 ccacttttga agcatcgaat cagttaagcc gctggcgatt agaagggaaa agagtggtgt
     3661 ttcgttttgt cacagatttt cctggagatg aaaatcatat tgatattcct gattggcttt
     3721 ttgaaaaaac tggtggcgac ggtacttttt atgataatga atatgggagg ggttttatcc
     3781 ctgattatgc taatccaaat tttatgtatt accaccatag ggctattaaa attttggggg
     3841 aacattatgg cggtgatgac ttctttgcat ttattgagtt gggtagcctt ggacattggg
     3901 gagaatggca tacttatgac atgcagttgt tcccggagga agacgttcgt aataaatatg
     3961 ttttagatta tgttgaggca ttcccagata ctcatttact tatgcggcgt ccatttaaaa
     4021 ttgcggatga tttaaacttc ggcttgtata atgacatgac gggtaattct aaagcgacaa
     4081 ctgaatggct tgactggatt gataagggcg gaagtttttt acctcaagaa aacggtgggc
     4141 ttgtttcaat gtcaaatgct tgggagctag ctccaattgg aggcgagcaa gcaccaactg
     4201 ttaagaatgc tgaaatgtat gcttcagaac ttgcatcaac tcttttattg ttgaaacaat
     4261 cccatactac atttatcggg ccaggcggac cttatgatgt tcctttagat agtccattgc
     4321 aagctggaat tgatgaagtg ctttctgtaa ttggttatag actttatatt tctcgtgttg
     4381 aaaccccaac agttgttcgg tttcaccatt ctattaaagt taaaattgaa ttctctaact
     4441 ctggcattgc tccattttat tatccttgga aggtttattt atacttattt gatgaagagg
     4501 ggaacatagc taaaaaagat ttgctaccga ttgatgtgcg aaggattcta ccagggcaaa
     4561 cattttctat ttcttatcta ttgccaagta agaaccttga taatggtaca tatactattg
     4621 gcattgcaat tattgaccct ctaactaatg ctcctgggat tagattagcc aataacaacg
     4681 agcgcactga cttgattcag caaattggat cttttcaagt taaatggata gataattttt
     4741 ttgtgactaa ataaaaccct gactttttat ggaactcccc aactttactt ctaagcataa
     4801 ggattttaat aaaacaggcg ccccaggcag gagtcgaacc cacaacctac tgatccgaag
     4861 tcaggcgctc tatccattga gctactgggg cgtgaccgcg attatacccg atttatgata
     4921 cttgcctcga aataaaaatc gaggcaagtt ttcgtttaca attcaaagat gctttcgaat
     4981 tcccttgccc ttgtcactgg atctgctcag cgattgggca aagcctttgc cctaacccta
     5041 gctcgtatgg gatattctat cgccttgcat tatcgtgggt cggtgacaga ggcggagaag
     5101 accactgcgg agattcgtgc attgggtgta gattgtttac ccattcgcgc agatttgact
     5161 gtgccagata aaatagaatt cctgttttca atggtagatg aatttcatct tcccctcaaa
     5221 gttgtggtca attcggctgc ccttatgccg gtgggaaacc cacgcgacct tgaacttaat
     5281 gattggaatt ctgcacttga cctcaaccta cgcgccccgt tcctgattgc gcaagaagcc
     5341 gccaagcgaa tggagcaggg tgggctgatc ataaatattt cagatgttgg ggcgcaaaaa
     5401 ccttggagcc gctatccttc gtacactgtg agtaaagccg gcgtcgagtc cttaaccaaa
     5461 atgctggcac gtgcgtttgc cccttccatt cgtgtcaatg cgattgcacc gggtttggtt
     5521 cttccttcag atgttgttac ccctgagaaa tgggagcagc ttgtaaagag acttccttta
     5581 caacgtgccg cgactttgga agagatcaca acgactctcg aatttctaat caaaaatgag
     5641 tatattaccg ggcaaaccat tgtcgttgat ggtggatatt cattaatcta aataacaaat
     5701 cctattattt cgaaaaccct gatgaactat aaaaaacttc ttcccgatat tcttgtctct
     5761 gctttgcttc ttgcttcagt tctctattat gcctttaatt acatcaattt cgatattcca
     5821 ccttttgaag atgccgccat gatcatgcgt tattcgcagc atcttgcggg tgggcatggc
     5881 attgtttgga atatcggcga acatccagtg gacggcgcaa cagactttct tttcatggtg
     5941 gcagcagctg gtttgataaa acttgggcta acagttgggc aagccgtgcg cggtattggg
     6001 tttgcttcac atctgttaac ggtcttgctt atttattgga ccaatcgccg tgttcataat
     6061 ggcaatatac ttttctcatt tttaagcgga ctttatctcg tcgttggaac gggtctttcc
     6121 tacgtttctg catacttcgg cacgcctttc tttgccctcg ccgccgcctc tacatggact
     6181 ttgggcttga ttctgatgaa ggaagagaat ccacgctggt ggctttctct cgccttcgct
     6241 ctcagcggac tcgttactgg gctaattcgt cccgaggggg tcatccttgc ttctctcatg
     6301 cttctcgctg tcatcgtcat gcgtggactc aaacgttccg tctccattat cgcgatattt
     6361 ggaattgttt ttctaacttt gggcggttcg tatttcttat ggcgctggaa ttattttggc
     6421 tatccattgc cgaacccatt ttataaaaag ggagagggcg gttggtcttg gctgactttt
     6481 gatgcttcta tgctgaatat gctgagatta tctttgcccg ttgtgttggc atttattctc
     6541 ggttttcggt cgaacgaaac tcttaaaacc acattggctt atatggttcc gatcttt
//
LOCUS       DAOQJE010000066         6593 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_6519_length_6593_cov_6.485317, whole genome shotgun sequence.
ACCESSION   DAOQJE010000066 DAOQJE010000000
VERSION     DAOQJE010000066.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 6593)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6593)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6593
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6519_length_6593_cov_6.485317"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..653)
                     /locus_tag="PLT08_02810"
     CDS             complement(<1..653)
                     /locus_tag="PLT08_02810"
                     /inference="COORDINATES: protein motif:HMM:NF012879.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HNE03423.1"
                     /translation="MHKNITQVQLKNGMKVMLKEIHTAPIISSWIWYKVGSRDESTGK
                     TGISHWTEHMMFKGTKKFPGGILDKAISREGGRWNASTSRDSTRYYETMPAAKIDLAL
                     RLESDRMTKSIFDAKEVASERTVIISEREGSENEPMFSLGEAVQHASFRVHPYHHEII
                     GDMADLRTITRDDLYNHYRSYYVPNNAVLSLAGDFDTKSMLKRIKELFEPIPKGNVPP
                     "
     gene            complement(713..1645)
                     /locus_tag="PLT08_02815"
     CDS             complement(713..1645)
                     /locus_tag="PLT08_02815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE03424.1"
                     /translation="MTDEPIKEDPVQKFRKLIASFIPLSRLPKKSTQAEPKEELPVEE
                     IKEEVKVEENKKDALSALPKKSTPQNDMLGSLPKKGALPPKKAEPKPVETPAPAPSPA
                     STPTSKLAPASSSGYSVWGPRLWTTASVISLTINIILMIVLIILVISVYRLKLDMAKI
                     MDASTSVMGLPAGLYSNFEKMERASIQTNVEVNTSIPVKFDLQLNQQTNVVLSQDVTI
                     QNARVTVNTGGLNISQANTTIVLPAGTTLPVNLALTVPVDQQVPVTLNVPVDIKLKDT
                     QLNDPFVGLQEVIKPLYCLLEPTAVNMDGNPICQ"
     gene            1891..3201
                     /locus_tag="PLT08_02820"
     CDS             1891..3201
                     /locus_tag="PLT08_02820"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HNE03425.1"
                     /translation="MESQFFVAVIGAGPAGLFGARELANSGVRVALFNRDIKAGGLAE
                     YGIYLEKHTMKEGLRKQFRQALDMPNIDYYGNVAIGTKGDFTLDEIRALGFDAVLVAA
                     GAQGTKSLGLPGEELEGVYHAKDVVYGYNKLPPFSQRKFRYGKRAAIIGAGNVMVDIA
                     RYLINHQGVEEVTAVVRRGPAEVNFTKEEMKHLVAYLDMDEFEKELARVRPALDAIGQ
                     DPQIGRQKVLDALAKADPKVKEAKFKFDFLSSPSGMFGENGLLTQLEVEENILTERDG
                     KTSAKGTGKKHTLDVETVIFAIGDQVDANFGLPTEWGEFSKNKEPKFPIEGTSYESTT
                     EGIFVGGWSRKASEGLVGYARRDGTNAAKAVMQYLQTKQPSNASPEAVAAKVKALQKP
                     IVYKEDIKRLEAAEAEEAKKRELEWFKYGSNEEMLQAMGLIETA"
     assembly_gap    3255..3354
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <3355..3633
                     /locus_tag="PLT08_02825"
     CDS             <3355..3633
                     /locus_tag="PLT08_02825"
                     /inference="COORDINATES: protein motif:HMM:NF013382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HNE03426.1"
                     /translation="SKDVVIIFGVLHHIPDWRKAIDEIIRVLKPGGSLFLEEPRGVDI
                     KVFDAFFRWGHPNTDFGLNALDAHLTQRGLTIETKQWTPLLTMYHIKK"
     gene            complement(3641..4153)
                     /locus_tag="PLT08_02830"
     CDS             complement(3641..4153)
                     /locus_tag="PLT08_02830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE03427.1"
                     /translation="MEVIYSVSPLKAFSGSFLFLAFFFVLGGFGALSAIFSRRDRAGK
                     RIALGCASAIILLAGAGGAVATFMTYTGGQTTALVHVDKKRIVERNCDNASNTCIDYV
                     IEASDGQKYYTFGLAKDTWDVVQDQACYEFTYYPAKSLFGEYLQQDNPYADMYETTSN
                     IVEIKTAQCP"
     gene            complement(4171..5355)
                     /locus_tag="PLT08_02835"
     CDS             complement(4171..5355)
                     /locus_tag="PLT08_02835"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF014564.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HNE03428.1"
                     /translation="MQPLQNIRVLDLSRVLAGPYCTMVLGDLGAEVIKVEPPEGDETR
                     GWGPPFAGGESAYYLCVNRNKRGMVVNLKTEEGRDVLKKLAMQSDVFVENFRPGTLKK
                     FGLDFETLHELNPKLIYCSISGFGQTGPLKDMPGYDFMIQAMGGIMSVTGEPEGEPMK
                     VGVAVADLFAGQNAIISILAALQARTFTGEGQYIDIALFDSELGWLANVASNFLISGK
                     NPKRYGNAHANIVPYQSFQASDGWFVVAVGNDKQFESFCNVIETPGLSSDERFSTNSA
                     RVQNREVLIPLLKPIFMQKTVNEWLALIGDQFPCGPINTFDQVFSMPHVKEREMLIEM
                     EHPTIGALPLVGSPLKMGGTPVSYRLPPPLMGQHTHEILKEVLEFSDEAVQKLMNDGC
                     IK"
     gene            5366..6118
                     /locus_tag="PLT08_02840"
     CDS             5366..6118
                     /locus_tag="PLT08_02840"
                     /inference="COORDINATES: protein motif:HMM:NF013355.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE03429.1"
                     /translation="MKRDLYFSKPLINAAGSLGFTPDPRNGISLDSFGAFVTNPLSLR
                     PRLPTTHPALVEFPGGFLMHTGLPNPGFHATLKKYSAKWNRANLPIIVHLMADRPEET
                     QRMVRELENVENVMAVQLGFAPLLSDDIILMNLEMCLGEVPLIFALPHEQVLTLGRQL
                     VDGGASAISIASPRGALYDKNGNLITGRMYGQSLFPRALDMVRSAVMSGIPIIGSGGV
                     WTEMDVESMLIAGAIAVETDAQLWVPKEAPSP"
     gene            complement(6206..6415)
                     /locus_tag="PLT08_02845"
     CDS             complement(6206..6415)
                     /locus_tag="PLT08_02845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HNE03430.1"
                     /translation="MAERIIGTVKWFNGTKGYGFIARDGGPDVFVHFSAIRGEGFKNL
                     QEGQKVEFTIEKGPKGLQAADVVVL"
BASE COUNT         1645 a         1585 c         1594 g         1669 t
ORIGIN      
        1 ggcgggacat ttcccttcgg gatcggctcg aacagttcct tgatccgttt tagcatggat
       61 ttggtatcaa aatctccagc cagcgaaagg acagcattgt tcgggacata gtatgaacga
      121 tagtggttgt aaaggtcatc gcgggtgatg gtgcgcaaat ctgccatgtc accgatgatt
      181 tcatgatgat acgggtggac acggaatgaa gcatgttgca ccgcctcccc tagtgagaac
      241 ataggctcat tctctgaccc ttcgcgctct gaaatgatga ccgttctttc agatgccact
      301 tctttggcat cgaaaatgct cttggtcatg cggtctgatt cgaggcgcag ggcaaggtca
      361 attttggcgg cgggcatggt ttcgtaatag cgggtggaat cgcgagaagt ggatgcgttc
      421 caacgtccgc cttcacggga gatggcttta tcgagaatac cgcctgggaa tttcttcgtc
      481 cctttgaaca tcatgtgttc tgtccaatga gagatgccgg tcttgccggt ggactcatcg
      541 cgcgagccga ctttgtacca gatccacgac gagatgatgg gtgccgtatg gatctctttg
      601 agcatgacct tcatgccatt cttaagctgg acctgcgtga tgtttttatg catttaactc
      661 caaagtattt tctgttattt tgcatcctca gacggtgcac aacaagcaag tattactggc
      721 agatcgggtt gccgtccata ttgacggcag tgggttcgag caaacagtaa agcggtttga
      781 tgacttcttg taagccaaca aaggggtcgt tcaattgtgt gtctttgagc ttgatatcaa
      841 cagggacgtt caaggtcact ggcacttgct gatctacggg gacggtaagg gctaagttga
      901 caggtaatgt agtgcccgcc gggaggacaa tggtggtgtt ggcttgcgaa atattcaacc
      961 cgccggtgtt gacggtgaca cgcgcgtttt gaatggtcac atcttgactc aagacgacat
     1021 tggtttgttg gttgagttgc aaatcaaatt tgaccggaat cgaggtgttg acctctacat
     1081 tcgtctgaat gctggcgcgt tccatctttt cgaaattgga atataagcca gcgggaagtc
     1141 ccatcacgga ggtggaagcg tccatgatct tcgccatatc tagtttgaga cgatagacgc
     1201 tgatcaccag aatgataaga acgatcatca gaatgatgtt gatcgtaaga gagataacgc
     1261 ttgccgttgt ccataaacga ggaccccaaa cggagtatcc gctactcgaa gcaggtgcca
     1321 gtttcgaggt tggggttgag gcggggctgg gggcgggagc cggagtctct accggtttcg
     1381 gttcagcttt tttaggcggt aacgctccct ttttgggtaa cgaccccagc atatcatttt
     1441 gtggcgttga ttttttgggt aaagctgaaa gagcgtcttt tttattttct tccactttga
     1501 cctcttcttt tatttcttcc acgggtagtt cttctttagg ctctgcctgc gttgattttt
     1561 taggcaggcg tgagagtgga atgaaactag caataagctt tcggaatttt tgtacaggat
     1621 cttctttgat gggttcgtct gtcatactgc ctcttggtta gcacatggat taatattagc
     1681 atagatgttt atgaagatgg ggtgggtttc ttacagcaag cacaaacatc tctaatgcgg
     1741 aattaacatc tgaaaagtaa ctttttgcag gtagcattcg actgtgataa cagaattgaa
     1801 cggttttttg tctgtaaatt ctgtgatgtt tgtggtaaaa cctgatagtt tttgtctaaa
     1861 atgtaatttt ttttatttcg gaggaagaac gtggagagtc aattttttgt cgccgtgatt
     1921 ggcgcgggac ccgcaggact tttcggggca cgtgaattag caaatagcgg agtgcgcgtt
     1981 gctcttttca atcgtgatat caaagctggt ggtctggctg agtacggcat ttaccttgaa
     2041 aaacacacaa tgaaagaagg gctgcgcaag caattccgtc aggcgctcga catgccgaac
     2101 attgactatt atggcaatgt agccatcggc acaaagggtg atttcacatt ggatgaaatt
     2161 cgcgcgctcg gttttgacgc ggtgttggtt gctgcgggtg cgcagggcac caagtctttg
     2221 ggcttgcccg gcgaagagct tgaaggcgtg taccacgcca aagacgtggt ttatggctat
     2281 aacaagttgc caccgttcag ccagcgcaag ttccgctatg gaaagcgtgc cgccattatc
     2341 ggcgctggta acgtgatggt ggatattgcc cgttatctca tcaaccacca aggtgtagaa
     2401 gaagtgaccg ccgtagttcg tcgcggtccg gctgaggtga atttcacgaa ggaagaaatg
     2461 aagcacctcg tcgcttatct cgacatggac gagttcgaga aggaattggc tcgtgtgcgc
     2521 ccggctctcg atgcgattgg tcaggacccg cagattgggc gtcaaaaggt tttggatgcg
     2581 cttgccaaag cagacccgaa ggtgaaagaa gcgaagttca agttcgactt cctctcctcc
     2641 ccttctggca tgtttggcga gaatggttta ctcacccaac tcgaagtgga agagaacatt
     2701 ctgaccgaac gcgatggaaa aaccagcgcc aagggcaccg gcaaaaagca tacgctcgat
     2761 gttgaaacag tcatcttcgc cattggagac caagtagatg caaattttgg tttgccgacc
     2821 gaatggggcg agttctccaa aaataaagag ccgaagttcc ccatcgaagg cacttcctac
     2881 gaatccacaa ccgaaggcat cttcgtgggc ggatggtctc gtaaggcaag cgaaggtttg
     2941 gttgggtatg ctcgccgcga tggcaccaat gccgccaaag ccgtgatgca atatctacag
     3001 accaaacagc ccagcaatgc cagcccggaa gcggtggcag ccaaggtcaa agcgctacaa
     3061 aagccgatcg tgtacaaaga agatatcaaa cggctcgaag cggctgaagc cgaagaagcc
     3121 aagaagcgtg aacttgaatg gttcaagtac ggcagcaacg aagaaatgtt gcaagccatg
     3181 ggtctgatcg aaaccgcgta agaaatcaat ctccgagttc ttcatgaact cggagatttt
     3241 ttatgaagct atctnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3301 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnagcaaa
     3361 gatgtcgtca ttatcttcgg tgtgctgcat cacatccctg attggcgcaa agcgattgac
     3421 gaaattatcc gcgtactcaa acccggcggc agcctttttc tcgaagaacc gcgcggtgtg
     3481 gatatcaaag tcttcgacgc tttcttccgt tggggacacc ccaacaccga tttcggactc
     3541 aacgccctag acgcgcatct gactcaacgc gggctgacca ttgaaaccaa acagtggact
     3601 ccgctgttga cgatgtatca catcaagaaa taggttctca tcaggggcac tgagccgttt
     3661 ttatttccac gatatttgag gttgtctcgt acatatcggc atagggatta tcttgttgca
     3721 aatactcacc gaacaatgat tttgccgggt aataagtgaa ttcataacaa gcctgatctt
     3781 ggactacatc ccaagtatct ttcgccaaac caaaagtgta atacttctgc ccgtcactgg
     3841 cttcaatgac ataatcaata caggtatttg aggcgttatc acaattacgt tctacgatcc
     3901 gctttttatc cacatgtaca agagcggttg tttgcccacc ggtataagtc ataaacgtag
     3961 ctactgctcc gcccgcccca gccaacagaa tgattgctga agcacaaccc aaggcaatgc
     4021 gtttccccgc tcggtcacgt cggctaaaga tggcagagag cgcgccaaac ccgcccaaca
     4081 caaagaaaaa tgctaaaaag agaaagcttc cagaaaatgc cttcaacggg gatacagaat
     4141 aaatgacttc catgtaaaac tccaataaag ttatttgata cacccgtcat tcatcaactt
     4201 ctgcaccgct tcatcggaaa actcaagcac ttctttcaaa atttcatggg tgtgctgacc
     4261 catcagcggc ggcgggaggc ggtatgagac cggcgtacca cccatcttaa gcggcgaacc
     4321 gaccagtggc aacgccccaa tggtgggatg ctccatctct atcaacatct cgcgttcttt
     4381 cacatgcggc atcgaaaaca cctggtcaaa agtattgatg ggtccacatg ggaattggtc
     4441 accaatgaga gcaagccatt cgttcacggt tttctgcatg aagataggtt tgagcaaagg
     4501 aattaatact tcgcgatttt gcacacgcgc cgaattcgta gaaaaccttt catcgctcga
     4561 aagcccaggg gtttcaatca cgttgcaaaa agactcaaac tgcttgtcat tccccactgc
     4621 caccacaaac caaccatcgc tggcttgaaa actttgatac ggcacaatat tcgcatgcgc
     4681 attgccataa cgcttggggt tcttccccga aatcagaaaa ttgcttgcaa catttgccag
     4741 ccaacccaat tcagaatcaa acaaggcaat gtcaatgtat tgaccttcgc cggtaaacgt
     4801 ccgcgcttgt aatgctgcca agatcgagat gatcgcattt tgcccggcga ataaatctgc
     4861 cacagccacg cccactttca tgggctcacc ctctggttcg ccagtgacgc tcatgattcc
     4921 gcccatggct tggatcataa aatcataccc gggcatatct ttcaacggac ccgtctgccc
     4981 gaagccggag attgagcaat aaatcagctt cgggttcaat tcatgcaaag tttcaaaatc
     5041 caagccaaat ttcttcaatg taccaggacg aaaattctcc acgaatacat cgctttgcat
     5101 ggcaagtttt tttagaacat ctcgtccctc ttccgttttc aaattcacca ccatgccacg
     5161 cttattgcgg tttacacata aataataagc gctctctcca cccgcaaatg gcggacccca
     5221 gccacgcgtt tcatcccctt cgggcggctc caccttgatg acctccgctc ccaagtcacc
     5281 aagaaccatc gtacaataag gtccagccaa cacacggctt aggtcgagca cgcggatatt
     5341 ctgtaagggc tgcatatcat ctcctatgaa acgcgatctg tattttagca aacctcttat
     5401 caatgcggcg ggatcattgg gattcacacc tgacccacgc aatggaatct cattggattc
     5461 gttcggggca ttcgtgacga atccgctctc tctccgcccg cgcctgccga ctacccatcc
     5521 ggctttggtc gaattccccg gcggattcct catgcacact gggctgccca accctggctt
     5581 tcatgccacg ctcaaaaaat attccgccaa atggaatcgt gccaacctgc ccatcatcgt
     5641 ccacttaatg gcagaccgcc ccgaagaaac tcaacgcatg gtgcgcgaac tggaaaatgt
     5701 tgaaaatgtg atggcggttc aactcggttt tgcgccactt ttatcagacg atattatctt
     5761 gatgaattta gaaatgtgct tgggcgaagt tccgcttatt tttgccttac cccatgagca
     5821 agtattaacc ttgggacgtc aactagtgga tggtggtgcg agtgccatca gtattgcctc
     5881 tccgcgcggc gcgttgtatg acaagaatgg caatctcatc accggcagaa tgtatggtca
     5941 gtcgctgttt ccgcgggcat tggatatggt ccgctcggca gtgatgagtg gaatcccaat
     6001 cattggttct ggcggggtgt ggacggagat ggatgtcgag tccatgctta tagcgggggc
     6061 aattgccgtc gaaacagatg cccagttatg ggtcccgaaa gaggcgcctt ctccctagcc
     6121 tccctaattt tttcaaaagg taacaaaaaa gagctccgtc tcacgacgaa gctctttcat
     6181 taggttcaat taattacttg cagaattaga gaaccactac atccgcggcc tgtaaacctt
     6241 tggggccctt ctcgatggtg aattccacct tctggccttc ttgcaggttc ttgaagcctt
     6301 cgccgcgaat agcggaaaag tggacgaaga cgtcgggtcc gccatcgcga gcgatgaaac
     6361 cataaccctt ggttccgttg aaccacttaa ccgtgccgat gatacgttca gccatatttg
     6421 cctcctgagc aaaatgaaaa aaaattgggt gacgcaattg cattcatcgg gtggtaaaaa
     6481 aaatacggct caaggtcttg ctttgagccg ttgtcttctt ccttccgcaa cgaaaacctc
     6541 ttgcatcctt caagccttaa tttatcatta aattccaaag ttggcaatta ttt
//
LOCUS       DAOQJE010000067         6528 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_6611_length_6528_cov_5.330450, whole genome shotgun sequence.
ACCESSION   DAOQJE010000067 DAOQJE010000000
VERSION     DAOQJE010000067.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 6528)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6528)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6528
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6611_length_6528_cov_5.330450"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1688)
                     /locus_tag="PLT08_02850"
     CDS             complement(<1..1688)
                     /locus_tag="PLT08_02850"
                     /inference="COORDINATES: protein motif:HMM:NF014602.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CarD family transcriptional regulator"
                     /protein_id="HNE03431.1"
                     /translation="MQQLLSQLRTLPSYQQTLSELTARKPVSGLGLPRAARLPLLAAL
                     HADINQPILLITDRADHALSMFDELGFWVKSPRYLFAEPNPLFYEEAAWGVTTRRERL
                     QTLTVLSTFHLPFTKKPETPPIIVASVRSLMTRTLPRRDFLKACKKLSVSQNHQPDSL
                     MREWARIGYQRVNTVLEPGQFSGRGGILDVWAPTELNPVRLDFFGDEIETIRSFDPAS
                     QRTLEKLENILITPSREFLADTTDSEAQLSEFHIPLLHQQPASLLDYLPQKTLVLVDD
                     GSLIEVMANEVEEQAVKFRQESIKEGTLAESFPVPYVPWSELNDNLSEFVHLELGHST
                     EAEIEIPLASHFGHDERFGGRLKPFIDYLAPIVDDGEQVFIVSRQAQRLRELWFEHYP
                     DSEHTNLEFTEASLSEGFTLKDQSLLSIHLITDSEVFGWERPQPRTRQRQTADTPESM
                     YADLEAGDYVVHVDHGIGRFAGLIQRQLDGHEREYLTVEYERGDILYVPVHQADRLTR
                     YVGADGGKPSLDNLGGQAWAETKEKVKEAVQKVAEDLLDLYARRQVVEGYAFSP"
     gene            complement(1703..2281)
                     /gene="pth"
                     /locus_tag="PLT08_02855"
     CDS             complement(1703..2281)
                     /gene="pth"
                     /locus_tag="PLT08_02855"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HNE03432.1"
                     /translation="MTETFLIIGLGNPGREYKDTRHNIGFMMIDHLAETIGARGMKVQ
                     SKAIVISGLHQERKLILAKPQTYMNLSGQSVQGLLHFYKIPLENLIIAHDDMDIPFGT
                     IRIRPTGGPGGQRGMANTIELLGTKDFPRLRLGIGRPPGRMDPKDYVLQDFSKDELKL
                     MPELLTRASEAALEFVTKGLNAAMNKYNGSVE"
     gene            complement(2296..2925)
                     /locus_tag="PLT08_02860"
     CDS             complement(2296..2925)
                     /locus_tag="PLT08_02860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE03433.1"
                     /translation="MLNLPIAMLKLFLILFLIFQGNTPSITSPQTGETLRGQVQIQGL
                     MDIPNFASAELAFTFDASASDPAASWFPIQTFPQPIQNPTLATWDTTAVTDGEYALRL
                     RIFLLDGTSQDVIVNTLKIRNNEPLPTPTITPTEAVFEFQPLDQTPGVSNEQNTPTPV
                     VLYSTATPLPANPASLTTSSIVTIFWQSAVLVVILFAFFSLLLRLRKNT"
     assembly_gap    3161..3260
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            3469..>4472
                     /locus_tag="PLT08_02865"
     CDS             3469..>4472
                     /locus_tag="PLT08_02865"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006241 - CTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HNE03434.1"
                     /translation="MATKYLFFTGGVVSSVGKGVTAAATGLLLKERGFKVAVQKLDPY
                     INVDPGTMSPYQHGEVYVLDDGAETDLDLGHYERFIDIRLSRVSNFTTGQVYAETIAK
                     ERRGDYLGGTIQVIPHITNEIKRRIGLAAKETGAEIMILEVGGTVGDIESQPFLEALR
                     QLRNEVGRENCLFIHVTWLPVIGATGEIKTKPTQHSVAALRSIGISPNVIIARADQDV
                     DQGLCDKIALFCDVEKDAVIPMKTASVLYEVPLLLDQLGVGELILNKLGLQATRKPDM
                     RPWKKLVDEVKKEKPTVNVALVGKYVELQDAYMSVREALKHAALANGVEISISWVHSA
                     "
     assembly_gap    4473..4572
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <4573..4902
                     /locus_tag="PLT08_02870"
     CDS             <4573..4902
                     /locus_tag="PLT08_02870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HNE03435.1"
                     /translation="DMGGTMRLGLYPCLLQPGTKAANAYSVESVDERHRHRWEFNNHY
                     REDFEKHGMVFSGLSPDGRLVEIVELKDHPYMVASQFHPEFLSRPMSPHPLFVGLLKA
                     AKEKAGK"
     gene            complement(4960..5658)
                     /locus_tag="PLT08_02875"
     CDS             complement(4960..5658)
                     /locus_tag="PLT08_02875"
                     /inference="COORDINATES: protein motif:HMM:NF027484.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NBR1-Ig-like domain-containing protein"
                     /protein_id="HNE03436.1"
                     /translation="MKLTYKIFLLFLMSTLFLVACGGNDAAATPELSGTGAVETLVAS
                     FFATQTAMYTPPPTNTNTSTPTNTALPTPTIPSNTPPPLATWTPVIVYASSTANLALT
                     PSVTGTPPTATQNTGALGFGCNNLAIVRDVNYPSGTTVKAGEVITKTWKVQNTGSCDW
                     QYNYALVIISDEYFNSGWSRLGKDVAPGSWSEISVTLGMPNHDGTFTGSWRLADAGGN
                     AFGATLSVSIVVVE"
     gene            complement(5728..6240)
                     /locus_tag="PLT08_02880"
     CDS             complement(5728..6240)
                     /locus_tag="PLT08_02880"
                     /inference="COORDINATES: protein motif:HMM:NF025056.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003953 - NAD+ nucleosidase activity
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="toll/interleukin-1 receptor domain-containing
                     protein"
                     /protein_id="HNE03437.1"
                     /translation="MPENSQHGANIMPQEERKHKEPIKDKLIPFLAYSKLNKTPVREF
                     AARLKAEGWIDPWFDEEDILPGQIWQNSVVTGVRQSHAVIIFLSKVAIASAGFYQKEI
                     KLALDTASEKPEGTIFIIPIRLNDCEVPEMLSKYQYIDYFGGLEQKEKVYSSLILSLK
                     LRAENLGIKV"
     gene            complement(6253..>6528)
                     /locus_tag="PLT08_02885"
     CDS             complement(6253..>6528)
                     /locus_tag="PLT08_02885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE03438.1"
                     /translation="KGKGLGVAKTGSARLALDANCPIVPMAVIGTDKFFKRFPFRTKV
                     IIKLLPPMLPKPGETPLALMDRVMFSLAKELPTEMRGVYSEVPKGFE"
BASE COUNT         1692 a         1456 c         1606 g         1574 t
ORIGIN      
        1 tcgggagaaa aagcatagcc ctctactact tgtctacgcg catataaatc cagtaagtct
       61 tcggctactt tctgaaccgc ttccttaacc ttttccttgg tctctgccca tgcctgcccg
      121 cctaagttat ctaacgaagg cttgccacca tcggcgccaa catagcgcgt aagtcgatcc
      181 gcttgatgga cgggaacata gagaatatcg ccgcgctcat attcaacggt gaggtattct
      241 cgctcgtgtc cgtccaactg tcgctggata agacccgcga aacgaccaat gccatgatcg
      301 acatggacga cgtaatcccc cgcttccaga tccgcataca ttgattcggg tgtatctgct
      361 gtttgacgtt ggcgtgtgcg cggctgtgga cgttcccagc caaagacttc tgagtcagta
      421 atgagatgaa tagagagtag agattgatct ttgagcgtga aaccttcaga aagtgaggct
      481 tcagtaaact cgaggtttgt atgttctgag tcagggtaat gctcaaacca caattcgcgc
      541 aaccgttgcg cctgacgcga aacgataaag acctgctcac catcatccac aatcggagca
      601 aggtaatcaa taaatggttt gagccgccca ccaaaacgct cgtcatgacc gaaatgcgaa
      661 gcaagcggaa tttcaatttc agcctctgta gaatgcccca actcaagatg aacgaattca
      721 ctcaagttat cgttcaactc agaccatggc acatacggca cagggaaact ctcggcgagc
      781 gtcccctctt tgatgctttc ctgcctgaat ttcaccgcct gctcttccac ttcatttgcc
      841 atcacttcga tcaggctgcc atcatccacc agaaccaagg tcttttgcgg caaataatcc
      901 aacaacgatg ctggttgttg gtgaagcaaa ggaatatgaa attcagagag ctgcgcttcc
      961 gagtcggtgg tatctgctaa aaattcgcgc gaaggcgtaa taagaatatt ttcaagtttc
     1021 tcaagcgtgc gctgtgaagc cgggtcaaaa ctgcgaatgg tttcgatctc atcgccaaag
     1081 aaatcaagcc gcaccggatt cagttccgtg ggcgcccaca catcgaggat gccaccacgc
     1141 ccagagaatt gacccggctc caagaccgtg ttgacacgct ggtagccgat ccgcgcccac
     1201 tcgcgcatca gactatcggg ttggtggttc tggcttactg acagcttttt acacgctttg
     1261 agaaagtctc gccgcggcaa agtacgcgtc atcaacgaac gaaccgatgc aacaataatg
     1321 ggcggagttt caggtttctt tgtaaatggc aagtggaatg tagaaagaac cgtaagcgtt
     1381 tgtaaccgtt cacgccgggt ggtcactccc caagccgcct cttcatagaa aagcgggttt
     1441 ggctccgcaa aaagataacg cggcgacttc acccaaaagc caagctcatc gaacatagac
     1501 aaggcatggt cggcacgatc ggtgatcagc aagatgggct gattaatatc cgcgtgaaga
     1561 gcagctaaca atggcaaacg tgccgcacgt ggcaagccca atccagagac gggtttacgt
     1621 gcggttagct cgctcagcgt ttgctgatac gaagggagag tgcgaagttg agaaagaagc
     1681 tgttgcatgg actctaaaaa atttattcta ccgagccgtt gtatttattc atcgccgcgt
     1741 tcaaaccctt ggttacaaat tcaagggccg cctctgacgc acgagtgaga agctcgggca
     1801 tgagtttcag ctcgtcttta gagaaatctt gcaaaacgta atccttcggg tccatacgcc
     1861 cgggcgggcg accaatgcca agccgcaagc gtggaaagtc tttagtgcca agtaattcaa
     1921 tggtatttgc catgccgcgc tgaccaccgg gtccgccggt gggacgaatg cgaatggtgc
     1981 cgaatggaat gtccatatcg tcgtgcgcaa tgatcaggtt ctcaagcgga attttataga
     2041 aatgcaaaag tccctgcacc gattgtcctg aaagattcat gtaggtctga ggcttggcga
     2101 ggatcaattt gcgttcctga tgcaaaccag aaatgacgat ggctttggat tgaaccttca
     2161 ttccgcgcgc gccaattgtc tcggcaagat ggtcgatcat cataaaaccg atattgtggc
     2221 gggtgtcttt atattcacgc cccgggtttc cgagaccaat aatgagaaaa gtttctgtca
     2281 tgagaaaaat ccaagctagg tattttttcg aagtcgaaga aggagggaga aaaaagcaaa
     2341 caggatcaca accaatacgg cgctttgcca aaaaatagta acgatggatg aagttgtcaa
     2401 cgaggctggg ttagcgggca aaggagttgc agtggaatat aaaacgacag gtgttggggt
     2461 attttgttcg ttggatactc caggagtttg gtcgagcggt tgaaattcaa agacggcttc
     2521 tgttggggta atggtcggtg tgggaagcgg ttcattattg cgaattttca gggtgttgac
     2581 aatgacatct tgagaggtgc cgtctaggag gaagatgcgt aatcgcaaag catattcccc
     2641 atcggtgacg gcggttgtat cccaagtggc gagggtggga ttctgaatcg gctggggaaa
     2701 ggtttggatg ggaaaccagc ttgcggcggg gtctgaggcg gaagcgtcaa acgtaaaagc
     2761 caactctgcc gaggcaaagt tgggaatatc catcagccct tgaatttgga cttgtccacg
     2821 cagggtttcg cccgtttgtg gggatgtgat tgaaggtgta tttccctgaa aaatgaggaa
     2881 aagaatcaaa aacagcttga gcatggcaat cggtaagttt aacatgaaga gaaggcaagg
     2941 tcaaattttg cctatttttc tgtaatcttt agattgaaaa tgaattgaaa tgtgggggca
     3001 attctcgaat gaagattttt ctggtaaact ctagccgttc aaagaaagta aacgtttatt
     3061 aggagattta agttatggca ccatcgcgcg ctgaacaact cgtggaacga cttcgcaaca
     3121 tgcaagccgc cgccccagac attgaagctt ctgccattgt nnnnnnnnnn nnnnnnnnnn
     3181 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3241 nnnnnnnnnn nnnnnnnnnn ctcggtatgg tcttcctcga aatgaggcga gccgctgaag
     3301 atctggtgaa gttagtcgga tgagacgtaa aagtcactcc acttcaaaat aaggtaaccc
     3361 cgaatgggga gggcataacg ccctccccat tctctttgta tataccccct cattcgcctt
     3421 tcctgtctgt gcaacagggt gggctgtaaa accaaggaga agaaaaggat ggcaacaaag
     3481 tatttatttt ttacgggcgg tgtggtttcg tcggtgggca aaggcgtgac tgcggcagca
     3541 accggtttgc tgcttaagga acgcggcttc aaagtggcgg ttcaaaaact ggacccgtac
     3601 atcaatgtgg acccgggcac gatgtcgccg tatcaacacg gtgaagtgta tgtccttgat
     3661 gacggcgcag aaacggacct tgatctgggt cactacgaac gattcattga catccgcttg
     3721 agccgagtca gcaacttcac aaccgggcag gtctatgctg agaccattgc caaggaacgt
     3781 cgtggtgact atctcggcgg gacgattcag gtcattccgc acatcacgaa cgagattaaa
     3841 cgtcgtattg ggttggcagc gaaagaaacc ggtgccgaaa tcatgattct cgaagtaggc
     3901 ggtacggttg gggatattga gtcgcagccg tttcttgaag cacttcgcca attgagaaat
     3961 gaggttgggc gtgaaaactg cttattcatt cacgtcactt ggctgccagt gatcggtgcg
     4021 accggtgaaa tcaagaccaa accgactcaa cactctgtgg cggctctacg ctccattggt
     4081 atttcgccaa atgtcatcat cgcccgcgcc gaccaagatg ttgaccaagg cttatgcgac
     4141 aaaattgcct tgttctgtga tgtagaaaaa gatgcggtta ttccaatgaa gaccgccagt
     4201 gtcctttatg aagtgcccct gcttttagac caacttggtg tgggcgaact cattctcaat
     4261 aaactgggtc tacaagctac acgtaagccg gatatgcgcc catggaagaa attggtggat
     4321 gaggttaaga aagaaaagcc caccgtcaac gtggcgttag tcggcaaata cgttgaattg
     4381 caagatgcct atatgtcggt tcgtgaggcg ttaaaacatg ccgccctcgc aaatggcgta
     4441 gagatttcca ttagctgggt tcattcagca gannnnnnnn nnnnnnnnnn nnnnnnnnnn
     4501 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4561 nnnnnnnnnn nngatatggg cggaaccatg cgcctcggac tttacccatg cctgctccaa
     4621 ccggggacga aagccgccaa cgcctatagc gtcgaaagtg tggacgaacg ccatcgtcac
     4681 cgctgggagt tcaacaatca ttaccgagaa gactttgaaa aacacggcat ggtgttctct
     4741 ggactctccc ccgatggtcg cttagttgaa attgttgaat tgaaagatca tccttacatg
     4801 gtcgcgagtc agttccaccc tgaattcttg tcccgcccga tgagcccaca tccgctattt
     4861 gtgggattac taaaagcagc aaaagaaaaa gccggcaagt aattactaaa acaccctccg
     4921 aaatttggag ggtgttttag taaagaaaat tattttcttt tattccacta caacaattga
     4981 aacactcaat gtcgcaccaa aagcgtttcc gcctgcgtct gccaaacgcc acgaaccagt
     5041 gaaagtgccg tcatgattag gcatccccaa tgtgaccgat atttctgacc aactgcctgg
     5101 agctacatcc ttcccaaggc ggctccaacc tgagttgaag tattcatcgc taataatcac
     5161 taaagcgtag ttatattgcc aatcacatga gcctgtgttc tgaaccttcc aggttttggt
     5221 aataacttcg cctgccttga ctgttgtacc gcttggatag ttcacatcgc gaacaatagc
     5281 taaattattg cagccaaaac ccaaagcccc tgtattttga gttgcggtgg gaggcgtccc
     5341 cgtcacggat ggagtcaagg caaggttggc agtagaactg gcatacacaa taaccggcgt
     5401 ccatgtggca agcggaggcg gagtattcga cggtatcgtg ggtgttggta aggcagtgtt
     5461 tgttggagtt gaggtattcg tattggttgg tggcggggtg tacatggcag tttgtgtagc
     5521 aaagaagcta gccactaatg tttccactgc tccagttcca gagagttctg gggtagccgc
     5581 cgcatcattc ccaccacagg caactaaaaa aagtgtggac attaaaaaca gtaaaaatat
     5641 cttatatgtg agtttcattg agataacctt tctatgtaat gattataaaa tgaaacttca
     5701 attcggattt agaaccacag aatggattta aaccttgatc cccaaatttt cagctctcaa
     5761 ttttaaagaa aggatcaaac tggaatacac tttttctttt tgttccaagc caccgaaata
     5821 atcaatatat tggtatttac tcaacatttc agggacttcg cagtcattta ggcgaatagg
     5881 aataatgaaa atcgtaccct ctggcttttc tgaagcggta tcaagggcta acttgatctc
     5941 tttttggtaa aaaccagccg aagcaattgc aacctttgaa aggaaaataa taacagcatg
     6001 ggactgtctc accccggtga caacggaatt ttgccaaatt tgccccggca aaatatcttc
     6061 ttcgtcaaac caagggtcaa tccagccctc ggctttgaga cgggctgcaa attcacgaac
     6121 aggggtttta ttgagcttgg aatacgcaag aaatggaatg agtttgtctt tgattggttc
     6181 tttgtgttta cgttcttctt gcggcatgat gttggctccg tgttgtgagt tttcaggcat
     6241 tatatcgcag cgttattcaa atcccttcgg cacctcggaa tagactccgc gcatttcagt
     6301 gggcaattct ttagcaagcg aaaacataac tcgatccatc aatgcaagtg gggtttcgcc
     6361 aggttttggc aacatgggtg gcaggagttt aatgattact ttagtacgga acgggaagcg
     6421 tttgaaaaac ttgtcggtgc cgatcacagc catgggaacg atgggacagt ttgcgtcaag
     6481 cgcgaggcgg gcagagccgg ttttggcgac gccaagtccc tttccttt
//
LOCUS       DAOQJE010000068         6467 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_6696_length_6467_cov_6.671865, whole genome shotgun sequence.
ACCESSION   DAOQJE010000068 DAOQJE010000000
VERSION     DAOQJE010000068.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 6467)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6467)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6467
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6696_length_6467_cov_6.671865"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..749)
                     /locus_tag="PLT08_02890"
     CDS             complement(<1..749)
                     /locus_tag="PLT08_02890"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070007 - glutamic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PrsW family glutamic-type intramembrane
                     protease"
                     /protein_id="HNE03439.1"
                     /translation="MNESKSIHERKHVWRDEFLLVIGLCVFVGIVYALDNALHPVFTP
                     TTLLLTGVFLAIVPAFIWMIFFYLQDSAEPEPKGYVIGVFVLGGLLAASIGIPVVENL
                     FRVSHWIYTNTVATILGGILVVGFTQEFLKYAAVRYSIYHSDEYDEPTDGVIYATAAG
                     LGYATMLNINFVISNGGVDLGTGIIRMAVVALAHAAFSGITGYFLGRAKFESEQIWWM
                     PLGITLAAIFNGLFNWLQGIVTRPTITLSGST"
     gene            complement(868..1434)
                     /locus_tag="PLT08_02895"
     CDS             complement(868..1434)
                     /locus_tag="PLT08_02895"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HNE03440.1"
                     /translation="MFPFVKLTDDVVMIRPYEFGDEHELHAAIQESLPELNPWMSWAT
                     ERYTLNVARDFVAITRAEWSRGTLYSFAILNASTNEFLGGCGLSHFHPVYKFCNLGYW
                     VRTSKHGNGYAGCATKLVAKFAFERANAIRAEIVIAKDNHASKRVAEKVGAHYEGILL
                     NRMTVRENIYDAHMFSLLPSDFGLAAQL"
     gene            complement(1519..2175)
                     /locus_tag="PLT08_02900"
     CDS             complement(1519..2175)
                     /locus_tag="PLT08_02900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HNE03441.1"
                     /translation="MKKQRIILVDDHEVVRLGLKSLLERHPHFDVVGEAGSAREALEQ
                     TALLKPDVVVMDIRLPGTSGIEACEQIVAQFPNTKVIMLTSYAEDEMLFSAIRAGASG
                     YILKQIGSEDLIKALESVGRGEALLDPAVTQRVFQEVRRAVKEEEASAFAHLSQQEKH
                     VLLLVSEGKTNREIAKNLFLGEGTVRNYVSSILSKLNVNNRAEAAAYAVEHNLRDYIS
                     "
     assembly_gap    2392..2491
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2492..3042)
                     /locus_tag="PLT08_02905"
     CDS             complement(<2492..3042)
                     /locus_tag="PLT08_02905"
                     /inference="COORDINATES: protein motif:HMM:NF012334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HNE03442.1"
                     /translation="MDPLKGKVVLITGAGKGIGRKLAETLAAQGAIVAANDISPINVD
                     EVVNGILAKGGQAKAYLEDVAKKVGAQMIVKSVEDEFGSLDILINHAAVEPHTPLLNM
                     DEWDWHRVLDVNLTGAFLMSQTAGRVMKEKGSGVIINLVAGAGERSKEAGAFLASKAG
                     LDELSRQLARELNPHGVKVVSAST"
     gene            complement(3049..3753)
                     /locus_tag="PLT08_02910"
     CDS             complement(3049..3753)
                     /locus_tag="PLT08_02910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE03443.1"
                     /translation="MLKKIFPFLALAVIVAAAGIYLYFTYFRGRSSARIAQVFDWFQD
                     QTSRPELMMTSGLKCGDAPFIFPTNGLIGFIWDDSFRPGHRHSGLDIFSGTEVGVTPI
                     VAAYPGYLTREADWISTVIIRVPEDPLKPNRQIWVYYTHMADPNGNSFVSSAFPQGTK
                     EVFVEAGTFLGYMGNYSGDPANPVGIHLHISVVKDDGFGNYTNELDINNTYDPSPYFG
                     MPLNANENTDVIPVCN"
     gene            3869..4252
                     /locus_tag="PLT08_02915"
     CDS             3869..4252
                     /locus_tag="PLT08_02915"
                     /inference="COORDINATES: protein motif:HMM:NF012241.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HNE03444.1"
                     /translation="MTLYIQPYPFRRMVRRTVAQPQSRALSVNVREEDDAYVLYALVP
                     GLKAEDLNIQVIENVVSIEGEYKDEETSFLLSELPRGAFSRSLRMPSEIDADKVEARI
                     AEGVLTLTLPKAESAKPKKIKVNVH"
     gene            4334..5353
                     /locus_tag="PLT08_02920"
     CDS             4334..5353
                     /locus_tag="PLT08_02920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE03445.1"
                     /translation="MRVKQTLFAILFVAILAACGGEETPDIAAPTPVPSHTPAPSATP
                     IPTLSTPLALLILPTDMDQETSDLYQKTVYDLALASGFRFQVRNGITPEDLADPTLKV
                     VIALPPDPGVASYAPTAPNVQFLAVNIPNLTAGGNVSVLAPNTQVELPAFLAGYTLAM
                     MTYEYHIGMLYPEGDPNSQAALNAFSNGMRYYCGICDGIYIDPVSYPAILGVPANEDP
                     GKFGGYANVLINDNKVGGLYIYPTLATDDFLSYVGTQGVYQIGTTMPEPRPGGWLMTI
                     TPDTIKAIQTAWPQLIAGQGGQNVQSPLGIADVDTGVLTEAKLRLVQQVLDELIAGRI
                     LPSNP"
     gene            complement(5394..5819)
                     /locus_tag="PLT08_02925"
     CDS             complement(5394..5819)
                     /locus_tag="PLT08_02925"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNE03446.1"
                     /translation="MTVSNTSTQNSILIISQDVDMTRVWKNLFEQKNCRVISETTARD
                     GVHAARTLSPTLIILDLDMPDKDRLDLCRDLRASTNGTLLLINASHKQVDVLEYHRAG
                     VDETVSDSISPMALLVKSLAWLARQDWIVPRKQSAQLWA"
     gene            6169..>6467
                     /locus_tag="PLT08_02930"
     CDS             6169..>6467
                     /locus_tag="PLT08_02930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE03447.1"
                     /translation="MFTIETQLLEAQDVRLGPIDLETHPAVESRWTHDAEFMHLMELS
                     PSLPLSPAMVKKQYQAIQREMEIQKDLFYFTIRAREDDRLIGKALIEYVDWSHGNG"
BASE COUNT         1649 a         1618 c         1487 g         1613 t
ORIGIN      
        1 gtcgagccgc tcaaagtgat ggtcggtctg gtgacgatac cttgcaacca attgaataag
       61 ccgttgaaaa tggcagccag agtgatgccg aggggcatcc accaaatttg ttcagattcg
      121 aacttggcac gcccgaggaa gtagcccgta atgcccgaga atgccgcatg cgcaagggca
      181 acaactgcca tacgaatgat gcctgtgccc aagtccacgc cgccattgga gatgacaaag
      241 ttgatgttga gcatggtcgc atagcccaac ccggcagctg tggcgtagat cacaccgtca
      301 gtcggttcat cgtattcgtc ggaatgatag atgctatacc gaacggcagc atactttaga
      361 aactcttgtg tgaagcccac tacaagaatt ccgcctagaa tggtggcaac tgtattggta
      421 tagatccaat gagaaacgcg gaagaggttt tctacaacag gaataccgat ggacgccgct
      481 aaaagcccgc ccaatacgaa gacgccgatc acatagcctt tcggttcagg ttcggcgcta
      541 tcctgcagat agaagaaaat catccaaata aatgcaggga caatggcaag aaagacacct
      601 gtaagtagca aggtggtggg ggtaaacacg gggtgtaggg cattatctag ggcgtagacg
      661 atacccacaa aaacacataa gccgataacg agcaggaact cgtcacgcca tacatgcttc
      721 cgttcatgaa ttgatttgga ttcattcatg gaattctctc tccttagtaa gtgatccatc
      781 aaataacaac cccatcatag taaaaagatg ggtgtacgtc aatagagctg ggttacaaat
      841 gggggacaga ctgagcggag aaaatgctta cagttgggca gccagcccaa agtctgaggg
      901 gagaagcgag aacatatgcg cgtcataaat attctcgcgg acggtcatgc gattcagcaa
      961 aatcccttcg taatgcgctc ccactttttc tgctacacgt ttgctggcgt ggttatcctt
     1021 tgcaattacg atctctgcac gaatcgcatt ggcgcgttcg aaggcaaact ttgctacaag
     1081 ttttgtggca caaccggcgt atccattgcc atgcttggag gtccgcaccc aatagccgag
     1141 gttacaaaac ttatagaccg ggtgaaaatg gcttaagcca caaccaccaa gaaattcgtt
     1201 tgtgcttgca ttcagaatgg caaaagaata taaggtgccg cgtgaccatt ctgcacgcgt
     1261 gatggcgaca aaatcgcgcg cgacattgag cgtgtatcgt tcggttgccc aactcatcca
     1321 cggatttaac tccggtagcg attcttgaat cgcagcatgc agttcgtgtt catccccgaa
     1381 ctcatatggg cgtatcatca caacatcatc ggtcaatttt acaaaaggga acatagtttt
     1441 ttaggcggga ataaaaaaag agacacttct tcacgcgggg tgcgaaagtg tctctttcat
     1501 agtggctgag aaaacgaatt aggaaatgta atctctcaaa ttgtgttcca ccgcgtaggc
     1561 tgctgcttca gcgcggttgt tcacatttaa cttcgagagt atgctcgaaa cataattgcg
     1621 gaccgttcct tcaccgagga agaggttctt ggcgatctcg cgattggtct tgccttcaga
     1681 gacgagtaga agcacgtgtt tttcttgctg gcttaagtgt gcaaaggctg aagcttcttc
     1741 ttcctttacg gcgcggcgca cttcttggaa gacgcgctgg gtcacagcag ggtcaagtag
     1801 cgcttcgcca cgtcccactg attcgagggc tttgatcaaa tcttcgctgc caatttgttt
     1861 gagaatgtag cccgaagcac ccgcacgaat ggcagaaaag agcatttcat cttctgcata
     1921 cgaagtgagc atgatgactt ttgtgttggg aaattgcgca acaatctgtt cgcaagcttc
     1981 aatgccagag gttcccggca ggcgaatgtc catcaccacc acatcaggtt tgaggagagc
     2041 cgtctgttcc aacgcttcac gcgccgaccc agcttcaccc acaacatcaa aatgggggtg
     2101 tctctctaaa agcgacttca gtccgagccg cacaacttca tgatcgtcaa ccaagatgat
     2161 tctttgtttc ttcataatag gcactatttt accgcaggaa attggaaaaa cgggcaaaaa
     2221 ttatagggtt tgtgcttttt tcaaagcttt caaatgttct tcaatgtgtg ccaacccgcg
     2281 ttctgcccac aactgcaaag ttagaccctt gcccaaggct tcgtgtcggc tcaaacgaga
     2341 ccaaccttct tgtggcatgt cacccaatag atcgcagact tcattcatgc gnnnnnnnnn
     2401 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2461 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn ngtcgaagca gagaccacct tcacaccatg
     2521 cggatttaat tctcgtgcca actggcggga caattcatcc aagccggctt tgctggcaag
     2581 aaacgctccc gcctctttcg acctttcgcc tgctcctgcc accaagttga taatcacgcc
     2641 ggagcctttt tctttcatca cccgccccgc cgtttgagac attagaaaag cgcccgttag
     2701 atttacatcc aacacgcggt gccaatccca ttcatccata ttgagcaaag gggtatgcgg
     2761 ttccaccgct gcatggttga tcaatatatc aagcgaccca aattcatctt ccacgctttt
     2821 gacgatcatt tgcgcaccga cctttttggc aacatcttcc aaataagcct ttgcctgccc
     2881 tccctttgcg aggattccgt tcaccacctc gtccacatta attggggaga tgtcattggc
     2941 tgccacaatt gcaccttgag ccgccaaagt ttctgccaat ttgcgaccaa ttcccttgcc
     3001 tgctccggta attaaaacca ctttgccttt caatggatcc atcctttctt aattacacac
     3061 tggtattaca tctgtgtttt catttgcatt caacggcatc ccaaaatagg ggcttgggtc
     3121 gtaggtgttg ttaatgtcca actcgtttgt gtaattcccg aagccatcat ctttaaccac
     3181 cgaaatatgc aagtggattc ccactgggtt tgccgggtca ccggaataat tacccatata
     3241 cccaaggaat gtccctgctt caacaaagac ttctttggtg ccttgtggaa aggcagaaga
     3301 gacaaaagaa tttccatttg ggtctgccat atgggtgtaa tacacccata tctggcgatt
     3361 gggcttcaac gggtcttctg gcacacggat aattaccgta gaaatccaat ctgcttcgcg
     3421 agtgagataa cctgggtagg cagccacaat gggtgttacg ccaacctccg tcccactgaa
     3481 aatgtctaac ccgctgtggc ggtgaccggg gcggaatgaa tcgtcccaaa tgaagccgat
     3541 caagccattg gtggggaaga tgaatggagc atcgccacat ttcaagccgc tggtcatcat
     3601 cagctcaggg cgagaagtct ggtcttggaa ccagtcaaat acttgagcga ttcgcgcaga
     3661 agacctgccg cgaaaatagg tgaagtacaa gtaaatccct gccgcagcga caataacggc
     3721 aagagccaga aacggaaata tctttttcaa catccaaaag attataccgc cctgaacttt
     3781 cttataaaaa atctaccaag tattagcatt tttcttacat tgcagattta atattgcgtc
     3841 attgacataa acaaaaggag acataaacat gaccctctat atccaacctt atccgttccg
     3901 ccgtatggtt cgccgtaccg tcgctcaacc tcagtcccgc gctcttagtg ttaacgtccg
     3961 cgaagaagat gatgcgtatg tcctttatgc gctcgtcccc ggtttgaaag ctgaagacct
     4021 gaacattcag gtgatcgaaa atgttgtcag catcgaaggc gaatataagg atgaagaaac
     4081 atccttcctc ctgagcgagc ttccacgcgg agccttcagc cgcagcttgc gaatgccgtc
     4141 tgaaattgat gcagacaagg tcgaagccag aatcgcagaa ggcgtcctaa cgctgacact
     4201 gcccaaggca gaatcagcca aaccgaagaa gatcaaagtc aacgttcact aatcaagccg
     4261 caggtcacgc acaatgcgtg acctgttttt ttcccgttat aatgttccgg acattttgta
     4321 acggagaaat tcagtgcgcg taaaacaaac tctttttgca attcttttcg tcgcaatcct
     4381 agccgcttgt ggtggggaag agacgccgga tatcgcagcc ccgacgcccg tcccgtctca
     4441 tactccggcg ccatctgcga cgcccatccc aaccttatcc acgccgttag cattactcat
     4501 tctccccaca gacatggatc aggaaacatc ggacttatac caaaaaacgg tttatgacct
     4561 ggcattggct tctggcttcc gcttccaggt tcgcaatgga atcactcccg aagacctcgc
     4621 ggacccaacc ctgaaagtag tcattgcttt gcctcccgac cctggtgtgg caagttatgc
     4681 gccgactgca ccgaatgtgc aattccttgc tgtaaatatc cccaatctca ctgcgggcgg
     4741 aaatgttagc gttcttgccc cgaacaccca agtagagtta cctgcttttc tggcaggcta
     4801 tactttggcg atgatgacct atgaatatca tattggtatg ctctacccag aaggtgaccc
     4861 gaactcacag gctgccttga acgctttcag caacggcatg cgttattact gtggcatctg
     4921 tgatggtatt tatatcgacc ctgtaagtta cccagctatc ttgggtgtac ctgccaatga
     4981 agaccccggt aaatttggtg ggtatgccaa tgtattgatc aacgataaca aagtgggtgg
     5041 cttatacatt taccccactc ttgctacgga tgattttctc tcttatgtag gtacccaggg
     5101 tgtttatcag atcgggacga ccatgccaga acctcgcccc ggcggctggc taatgaccat
     5161 tacgcccgat actatcaaag ccattcaaac tgcttggccc cagttgattg ccgggcaagg
     5221 cggacagaac gttcagtctc cacttggtat cgcagatgtg gacactggcg ttcttacaga
     5281 agcaaagtta cgtcttgtgc agcaagtatt agatgagttg atcgcaggta gaattcttcc
     5341 ttccaaccca tagaattgag aaaaaaagac ccgcgaattt cgcgggtctt tttttatgcc
     5401 caaagttgcg ccgattgttt gcgcggaacg atccaatctt gccgtgccag ccatgccagt
     5461 gatttgacca ataacgccat tgggctgatc gagtccgata cggtttcatc cactccggcg
     5521 cggtgatatt cgagtacatc cacctgttta tggctggcat tgatgagcag cagtgtgcca
     5581 ttggtgctgg ctcgcaagtc gcggcaaagg tctagccggt ctttatctgg catatccaag
     5641 tcaagaataa tcagggtagg ggagagagtg cgtgccgcgt gtactccatc tcgcgctgtg
     5701 gtttcgctaa tgacgcggca atttttctgt tcgaataagt tcttccaaac acgggtcatg
     5761 tctacatctt gtgagatgat caggatgctg ttctgagttg aagtattgct aactgtcatg
     5821 gctacctctc tataaacact aggtcgttga aaactttgaa aagggtctta attcacctaa
     5881 atgtcctact tgactggatt tgttcgtgcg tgataaactc gcgttacctg aaaaaggaat
     5941 tctaaagaaa ggagcgcact acccatgttc atgatgcaat tcttcatttc cgcagctgcc
     6001 agtttcgctg gtgatagcag tagtgcgcct gttcgtcgtt aggtttaact gaaaccttcg
     6061 cgaccacggc gcgtcaagcc cgtggtcgtt tttctttaac cgccttagaa cggtcacggg
     6121 agagaacctc ttgtgaccgt tttttgttat ttagtaaaga aggaaatcat gtttacgatt
     6181 gaaacccagt tgcttgaagc acaggacgta cgtttaggtc ccatagacct tgaaacgcat
     6241 cccgctgtcg aatcccgctg gacgcacgac gccgaattta tgcacttaat ggagttgagt
     6301 ccctctctgc ccctttcacc tgcgatggtc aagaaacaat accaagccat tcaaagagaa
     6361 atggagatac agaaagacct cttctatttc accatccgcg cccgcgaaga tgaccgtctg
     6421 atcggcaaag ccctcattga atatgtggac tggtcgcatg gcaatgg
//
LOCUS       DAOQJE010000069         6444 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_6748_length_6444_cov_6.281421, whole genome shotgun sequence.
ACCESSION   DAOQJE010000069 DAOQJE010000000
VERSION     DAOQJE010000069.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 6444)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6444)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6444
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6748_length_6444_cov_6.281421"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..705
                     /locus_tag="PLT08_02935"
     CDS             <1..705
                     /locus_tag="PLT08_02935"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HNE03448.1"
                     /translation="YQNGFSVFRLNFRDHGETHHLNPGIFYAALLDEVFGAVQQVARY
                     EPALPFYVIGFSMGGNFALRIARKAEESPIPNLKHVIAISPVLDPEKSTYAIDEFPLL
                     RDYFRKKWTDSMRKKQALFPELYQLYDVFSLPTIAEMTERMLQRHSDYPSASAYFRAY
                     AVTGEALAALSTPLTVITAQDDPIIPVSDFHHLKLSSTGELLIQPYGGHNGFLENIFG
                     KAWYQRKIAEILLPLG"
     gene            764..2602
                     /locus_tag="PLT08_02940"
     CDS             764..2602
                     /locus_tag="PLT08_02940"
                     /inference="COORDINATES: protein motif:HMM:NF025056.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003953 - NAD+ nucleosidase activity
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="toll/interleukin-1 receptor domain-containing
                     protein"
                     /protein_id="HNE03449.1"
                     /translation="MKTSLMISYSRRQTPFVDRLYEELQKDGYSSIWLDYQSLTPAQP
                     WYQQILDGITGAETLLLVVSKDSIESKNVEPEWRLALEHKKRIVLVIFEAYPLPVELQ
                     GCEWVDFRLNHKASLQKLSNMLGMPPQPVTFPAPQSGFKAPKRFWLAIALSVIVLLSS
                     LPSWWTLIIPFILIPLPWQIYKRDYSFSRVIPTLTVLFPLFQIISVNWFFEEGSALSF
                     LRDIINLDWLPVVGVLAGWLLVGLLLTPVMQRRATPVAARVRFANPLVADIKKPQPVV
                     FAIDHAPEDGRYANALRRTLEKYGHRSIEPGETPQAVLVLISAFKKTTEYDLDTVAVY
                     PILLQAVTDIDRKLQRIQWIDFRKGMGSADKLAKLLPEPQKLLKVLAVAPTGSQEIFP
                     LAVNALQYFYLIAGVFGGGGLLISTLSFASLIASGVFGIEYISQIFVAILNGAFLFLV
                     AFSAVRGLRTRLGGSLALYPLLILMGFVYLINFANFSALFVDSNSEETSQLAFTAVNG
                     AGFALWFLPMGTFLIIPFLLFGWQGLYRWIPLRQTDNVTSLESIFLFYAPRKKGRLVF
                     HILFHLLLAVIVIAAMQLDSGEILTDLLVVVMMAVFFRWLARRNLK"
     gene            complement(2688..3191)
                     /locus_tag="PLT08_02945"
     CDS             complement(2688..3191)
                     /locus_tag="PLT08_02945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE03450.1"
                     /translation="MGLFGFGKKANKNAPVLSADEVRTKILELNRETAPYQIIDGKDK
                     GVDLIAEWKIVDAKWYELFAKANITKVFRIYMKFDPAKNEVRAKDEEYTVEWRAGVPS
                     LSISVSKFQGQMTSVEFGAAYAFTEELKPGLVYNYRFNTNEIKKPIQEAVATCGWNYK
                     GVAFGKL"
     gene            3327..3863
                     /locus_tag="PLT08_02950"
     CDS             3327..3863
                     /locus_tag="PLT08_02950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE03451.1"
                     /translation="MFFGKKETTEKKKNPIRLTILTDEYIIDALDDPDLSILQAVHDS
                     EIGDASGGVIILKDATLHSLRHASAPARTFSEWRLTSLVNMIAILSDDPVAQQVIAEG
                     WEDYQTPFKAFIYAGVYIIEGKMFSEDDTPPDFRIRTFAPLEDATITYALDQKEKTIR
                     GRWGLVNSVLMHGFSIEK"
     gene            3893..5293
                     /locus_tag="PLT08_02955"
     CDS             3893..5293
                     /locus_tag="PLT08_02955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE03452.1"
                     /translation="MRKIFFVISIFVLTALACQSLTSTPLPSEPLTDSSTQAVSAPQE
                     TALPIGTSTPTSLPKGVIFVDTLQQEVYPFEENGKCSLAEAVIAANTGAPKDTCPAGV
                     PGESIISLMPGEYHFSQRDQSPPQFDWLLSLVKVGSALPPITFPLVIQGNGATLVRDE
                     GSEPFRFFEVMVNASLSLENMTLQNGDVQDDSGGAIYASNGSVTLNRVRFLNNHAEHG
                     GAVYLTFGALTVQESEFIENHAGFQGGGIHLDSAKSTIQNTKFISNTTDARGGALSAE
                     GVTLVVEDSLFLKNVTQDNRGGALHLEHVNVNIARSQFYQNQSAWIGAAIYINNPVMS
                     GTSEDEGDPLEQLDDTPMYVQMATLIPGYEATLQAHPSGVFQDFHEDMQVHQNCFANN
                     IILDTYEMNFSSAILARDVNAEDNYYGTASGPSGSGPGTGDGVGRSVKFAPFLTEPPA
                     YCDPELSQQVKENHNQ"
     gene            5303..5563
                     /locus_tag="PLT08_02960"
     CDS             5303..5563
                     /locus_tag="PLT08_02960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE03453.1"
                     /translation="MAKLIPASERILRARKLIEQARALPVPTEGLGKSDFSYIANVRD
                     VLRQAKDMVKFIPQTAGVSLEMKEEVKKIYAEVEQAEKEILS"
     gene            5652..5909
                     /locus_tag="PLT08_02965"
     CDS             5652..5909
                     /locus_tag="PLT08_02965"
                     /inference="COORDINATES: protein motif:HMM:NF025715.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4404 family protein"
                     /protein_id="HNE03454.1"
                     /translation="MTDKLDALLEQVHAELQKVDEVDADSLKLLQELQQDVQGLLDKT
                     EMETPTVLARMQKAVQQFEVDHPALTALISELSTVLSNAGI"
     gene            6071..>6444
                     /locus_tag="PLT08_02970"
     CDS             6071..>6444
                     /locus_tag="PLT08_02970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE03455.1"
                     /translation="MKKTLVIVALVVTALAILGVGAVFAQDVTPPYAGHGPMMNGEGP
                     LHTFMVIEFAKKLDLNVNDINTRLDAGESMYDIALSTGLTAEEFHVVMTEVRTNAVNA
                     AVAANVITQEQADWMLSHGYGQG"
BASE COUNT         1556 a         1622 c         1483 g         1783 t
ORIGIN      
        1 taccaaaacg gcttctcggt cttccggctc aacttccgcg accacggcga gacacatcac
       61 ctaaaccccg gcatctttta cgccgcactt ctcgatgaag tctttggcgc ggtacaacaa
      121 gttgctcgtt acgaaccggc gcttccgttc tatgtgatcg gcttttcaat gggcgggaac
      181 ttcgccttgc gtatcgcccg caaagccgaa gagagtccca tcccgaatct caagcacgtc
      241 atcgccatca gcccggtgct cgaccccgaa aagtccacct acgcgattga tgaatttcct
      301 ctgctgcgcg attacttccg caaaaaatgg accgactcca tgcgcaagaa gcaggcactc
      361 ttccccgaac tttaccagtt atatgacgtc ttttccctcc cgaccattgc cgagatgacc
      421 gagcgcatgc tccaacgtca cagcgactat ccctccgcct ctgcctactt ccgcgcctat
      481 gccgtcactg gagaagcgct cgcggctctt tctacgccgc tcacggtcat caccgcccaa
      541 gacgatccca tcatccccgt ctccgacttt caccatctca aactatcgtc cacgggcgag
      601 cttctcatcc aaccctacgg cggtcacaac ggctttttag aaaatatctt tggcaaagcc
      661 tggtatcaac ggaagatcgc ggaaatctta ttaccgcttg gttgatacat tctctgctta
      721 tataatagat taaaaccaat tcaatcaacg gaggaagcca ttcatgaaaa ccagtttgat
      781 gatcagttat tcacgccgcc agactccttt tgtagaccga ctgtatgaag aactgcagaa
      841 agatgggtac tcttccatct ggttggatta tcaaagcttg acccctgccc agccctggta
      901 tcagcagatc ttagatggca ttactggggc agagacccta ttgttggtcg tttccaaaga
      961 ttcgattgaa tcaaaaaatg ttgaaccaga atggcggctg gcacttgagc ataagaagcg
     1021 catcgttttg gtaatctttg aagcctatcc cttgcctgtc gaattacaag gttgtgagtg
     1081 ggttgatttc cgcttgaatc ataaagccag tctccaaaag ctgtccaaca tgttgggtat
     1141 gcctccacaa ccagttacat ttcccgcacc ccaaagcgga ttcaaagctc ccaagcgctt
     1201 ctggctggca attgccctga gtgtaattgt cctgctttct tctctgcctt cctggtggac
     1261 gctcattatt cccttcatcc ttatcccgtt gccgtggcag atctataaaa gagactatag
     1321 tttctcgcgt gtcatcccca cgttgaccgt tctatttcct ttatttcaga taatttcggt
     1381 gaattggttt tttgaagaag gtagtgccct ttctttctta cgtgacataa taaatttgga
     1441 ttggctgcca gtcgttggtg ttcttgcagg ctggcttctt gttggcttac tgctcacccc
     1501 agtcatgcaa cgacgtgcca cacctgtggc ggcgcgtgtc cgttttgcca acccactggt
     1561 ggcagatatc aagaagccgc agccggtcgt ctttgcaatt gaccatgctc cagaagatgg
     1621 gcgttatgcc aacgctttac gacgcacgct ggaaaaatat gggcaccgtt ccatcgaacc
     1681 gggagaaacc cctcaggcag tgttggttct catttcggcg tttaagaaaa caacagaata
     1741 cgacctcgat actgtggcgg tttacccgat tctcttacaa gcagtcacag atattgatag
     1801 aaaacttcag cgcattcaat ggatcgactt ccgcaaaggg atgggttcgg cagataaatt
     1861 ggctaaactt ttgcctgagc cgcaaaagtt actaaaagtg ctggcagtcg ccccaacagg
     1921 ctcacaggaa atcttcccat tggcggtcaa tgctcttcaa tatttttatt tgattgcggg
     1981 tgttttcggg ggaggagggc ttttaatttc aaccctgtct tttgcatctt tgatcgcatc
     2041 tggagttttt ggcattgagt acatctctca aatctttgtt gctatcttaa atggtgcatt
     2101 tttatttctt gttgcctttt ctgctgtacg gggtcttcgc actcgtcttg gcggctcgct
     2161 tgcactatac cctttattga ttttaatggg ctttgtgtat cttattaact tcgctaattt
     2221 ttccgccttg tttgttgatt ccaattcaga ggagacttca cagctggcat ttacggctgt
     2281 caatggggct gggtttgcct tgtggttttt accgatgggc accttcctaa taattccctt
     2341 ccttcttttt ggctggcagg gcttatatcg ttggattccg cttcgccaaa ccgataatgt
     2401 cacctcattg gaaagtatat ttttattcta tgccccccgg aaaaaaggga ggcttgtttt
     2461 ccacattttg tttcacttgc tcttagcagt tattgtgatt gcagctatgc aattggattc
     2521 aggtgaaata ctcactgatt tgctagtagt agttatgatg gcagtatttt ttcgctggct
     2581 ggcgaggcgt aatttaaaat aaacattgcc ttgaatgaac aataaataac cacgccgttc
     2641 ttcttcaaga gcggcgtggt tatttatgca ggctttttaa acgagttcta cagcttaccg
     2701 aacgccacac ctttgtagtt ccaaccacaa gtcgctacgg cttcctgaat cggcttcttg
     2761 atttcatttg tattaaagcg atagttgtag accaatcccg gcttcaattc ttcggtaaac
     2821 gcataggctg caccaaattc caccgaagtc atctgtccct gaaacttcga gaccgagatc
     2881 gaaagcgacg gcacgcctgc ccgccattcc acggtgtatt cttcgtcctt ggcgcgcact
     2941 tcgttctttg ccgggtcgaa cttcatgtaa atgcgaaaga ccttggtgat gtttgccttc
     3001 gcaaagagtt cgtaccactt cgcatccacg atcttccact ccgcgatcag gtccacgcct
     3061 ttgtctttcc cgtcaatgat ttgatacggc gccgtttcac ggttcaattc caatatcttc
     3121 gtccgcactt cgtctgctga gagaaccggt gcgttcttgt ttgctttctt gccgaaacca
     3181 aagagtccca taacaatgtc ctttcgaatt gatgaactga ctttattata cgagttgctt
     3241 ccacattctc tatcccccag ataggcgatt ggggaaatcc ccgctccttc atataattgt
     3301 ctgcgtctat atggaaggag agtaccatgt tttttggtaa aaaagaaacc actgaaaaga
     3361 aaaagaatcc cattcgtcta accatcctca cagatgaata tattattgat gccttggatg
     3421 acccagacct atccattttg caggcagtcc acgactcaga aatcggggat gcatccggcg
     3481 gcgtaattat cttgaaagac gcgaccctac attcgcttcg gcacgcgtcg gctccggctc
     3541 gcaccttttc cgaatggcgg ttgacctctt tggtaaacat gatcgccatt ctttcggatg
     3601 atcctgtcgc acagcaggtg atcgcagaag gctgggaaga ttatcagacg cctttcaagg
     3661 cttttatcta cgcaggggtc tacatcattg aaggcaagat gttttcagaa gacgatactc
     3721 cgcccgattt taggattcgc acctttgccc cgctcgaaga cgccacaatc acctacgcat
     3781 tagaccaaaa agaaaagacc attcgcgggc gctggggatt ggtcaactcg gttctcatgc
     3841 atggctttag tattgaaaaa taaattttat cgtttctgtt ttggagtatg ttgtgagaaa
     3901 gatttttttt gtaatttcga ttttcgtttt aacagcgtta gcttgccagt ccctgacgag
     3961 taccccgctt ccttctgagc cgctgactga ttcatccacc caggctgttt ctgccccgca
     4021 agagacggct ctgcccatcg gcacgtccac gccaacttct ctgcccaaag gggttatctt
     4081 cgttgataca ttgcagcaag aagtgtatcc cttcgaagag aatggtaaat gttcactggc
     4141 agaggctgtc attgctgcca acacaggcgc acctaaagac acttgtcccg ctggtgtgcc
     4201 gggtgaatcc attatcagcc tcatgcccgg agaatatcat ttcagtcaac gtgaccaaag
     4261 tccgccgcaa tttgattggt tgctaagctt ggtcaaggtt ggcagtgcct tgccgccgat
     4321 tactttccca ttggttattc aaggtaacgg cgcgaccctt gtccgcgatg aaggctcgga
     4381 acccttccgc ttctttgaag tcatggtcaa tgccagcctc agccttgaga atatgacttt
     4441 gcaaaatggc gacgtacaag atgactcggg cggtgcaatc tatgcctcaa atgggtctgt
     4501 caccttgaac cgcgtccgtt ttttgaacaa tcatgctgag catggcgggg ctgtctattt
     4561 aacctttggg gcactgaccg ttcaagagag cgagtttatt gagaatcatg ctggctttca
     4621 aggtggcggt attcacctcg actctgccaa gtctaccatt cagaatacga aattcatcag
     4681 caacaccacc gacgcgcgcg gtggtgcgct ttctgccgag ggggttacct tggtggttga
     4741 agacagtttg ttcttgaaaa atgtaaccca agataatcgt ggcggcgctt tgcatttgga
     4801 acatgtcaac gtcaatatag cgcgcagtca gttttaccag aaccagtctg catggatcgg
     4861 agccgcgatc tacatcaaca acccggtgat gagcggtacc agcgaagatg aaggggatcc
     4921 gctcgaacag ttggatgata cgcctatgta tgttcaaatg gcgactctta ttcctggcta
     4981 tgaagcgacc ttgcaggctc accccagtgg ggtgtttcaa gactttcatg aagatatgca
     5041 ggtccatcag aattgttttg ccaataatat tattttggat acctatgaga tgaacttctc
     5101 aagcgccatc cttgcgcgtg acgtcaatgc cgaggataat tactacggca ccgctagcgg
     5161 accctccggc agtggtccag gcaccggaga tggagtaggt cggtctgtga aatttgcacc
     5221 cttccttacc gaaccgcctg cctattgcga ccctgagttg tcacagcaag tgaaagaaaa
     5281 tcataatcaa taggatttca tcatggcaaa acttatccct gcatctgaac gcatcttacg
     5341 tgcccgaaaa ctgatcgaac aagcccgggc tttacccgtc ccgacagaag gtcttgggaa
     5401 aagcgatttt tcctatatcg ccaatgtccg cgacgttttg cgtcaggcaa aggatatggt
     5461 caagttcatt ccacagacgg cgggtgtttc gctcgaaatg aaggaagagg tcaagaagat
     5521 ctacgctgaa gtggaacagg cagagaagga aatattgtca taatcaaggt tttatgacgt
     5581 tattcactgg aagcagcctt gggttggttg tacaatgaaa gtgaacctga tcctcgaaag
     5641 gagccaaccc catgaccgat aaactcgacg cacttctgga acaagtccat gccgagctcc
     5701 aaaaagtgga cgaagtggat gccgacagcc tcaaactttt acaagaactg caacaggatg
     5761 tgcaaggatt gcttgacaag accgagatgg agacgccaac cgtgcttgcc cgaatgcaaa
     5821 aggcggtgca gcaatttgaa gtagatcacc ctgctcttac ggctttgatc tctgaactat
     5881 ccacagtatt aagcaacgcg ggaatttgaa agagcccgag accgtcatga aggaattttc
     5941 cttcatgacg gtctttttta tttttgatga tcggaattct tcacctgacc tctacctttt
     6001 ctttacagaa cctttacatg cgggctgtac actttggctt gtaagttcga aaacgaaaag
     6061 gagaaataaa atgaaaaaga cacttgtgat cgttgcgttg gtagtgacag cgttagcaat
     6121 tttgggagta ggtgcggttt ttgctcagga cgtcaccccg ccttatgccg ggcatggacc
     6181 tatgatgaat ggggaaggtc cgcttcacac cttcatggtg attgaatttg ccaaaaaact
     6241 tgacttgaat gtaaacgaca tcaatacccg ccttgacgcc ggtgaaagca tgtacgatat
     6301 tgcactctca accgggctga ctgctgaaga attccatgtt gtgatgaccg aagttcgcac
     6361 caatgctgtc aatgcggcag ttgctgcaaa tgtgatcacc caggagcagg cagattggat
     6421 gctgtctcat ggatatggtc aggg
//
LOCUS       DAOQJE010000070         6389 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_6834_length_6389_cov_6.811178, whole genome shotgun sequence.
ACCESSION   DAOQJE010000070 DAOQJE010000000
VERSION     DAOQJE010000070.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 6389)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6389)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6389
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6834_length_6389_cov_6.811178"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1218
                     /locus_tag="PLT08_02975"
     CDS             <1..1218
                     /locus_tag="PLT08_02975"
                     /inference="COORDINATES: protein motif:HMM:NF012356.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 13 protein"
                     /protein_id="HNE03456.1"
                     /translation="FDYYQVDPLLGGNEALKKLLNAAHKRDIRVVLDGVFNHASRGFW
                     QFHHVLETGAASPYKDWFHFDHERLNGHRHWGAYPSSVEQKLLQHEDSLTAIGYRAWW
                     NLPALPKFNTNTPAVREFLFDVAEYWVKFGIDGWRLDVPGEIDDDEFWREFRRRVRAV
                     NPDAYIVGEIWHEAQRWLQGDQFDAVMNYLFTAASLNFFAGAHLDHEVIQHQGGIKDR
                     IHRMHGKAFADEVERILSLYPQDITFSQLNLLDSHDMPRFLSCAGGDKDSLKLAWLFI
                     FTMPGAPCIYYGDEIGVDGAHDPDCRKSFPWDQSKWDMNLLEYAKACISLRKGQKALR
                     RGEYKRVHAEDDMMAFARTYKEEAVVVAFNASNTPKSFEYKFEKNPRVLFGNPEVSGQ
                     HITVPPRSGVVLK"
     gene            1353..5120
                     /locus_tag="PLT08_02980"
     CDS             1353..5120
                     /locus_tag="PLT08_02980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE03457.1"
                     /translation="MNKSKEIQLAKASYNWLSISPILTVSTLFFVSSMGIGNTVCDNS
                     PIICGYDTSTYISFAIGIVLSALWHLILLQYTTNKESEFVRLHGQRALTHAGIRTGVA
                     LIGLGLDWASNASGGFACVAVLVLLLMWVINITQSKRRIEEDAKLFDVPNEQLEAPQT
                     ETTFSQEHVHAENMAMSGTDSRKPEEVLNEIYQNLQSEDDVVVIQAISTLTSLNYSSE
                     AIRRKLEQLSIQSDNKDIRAQALAALNSSANRAVQKHFIANKLDRGIRYTLLQEINDW
                     EKSNLLDKQNADVIRRRYDFDFTPAATTTSSAPQPQAIAAPAQPALATPQAAPPVTTS
                     TPPEPRPSLLQTLTSEASIKIYLYLGAFFVIAAAAILGAVVPELRLPILMIGTFIFGG
                     LSVVIKKRLPQPSFALFIVFSFLLPITANSLEESLRQAFNLTADFTDAYWAMIFIVMA
                     IIWIGSTWLYESRLFSLTAFGSLILASVRIGDLFEADAVYYTLLMGLTALVGLGGTWL
                     LKKWRDEKFAQPLFLASQLLQALNLATSLGIFGVNTFQSSGNNILNLITLATWITGTL
                     FYLVSEWLYPFFAFPWLAAATFIPMPWLIASAFDLESLGSSIVLFVWGAILAVASEPI
                     IRSEKLKKYGLPILLAAMPSLALGIFTGFIYETWLGLTLALGTAILFTSLHILQNRWW
                     LWTLALLNFVAAYFAFFQLEAIQKIDIFFGYQLLGITILYLLPDLFLKKDWMLNLQWR
                     LPLRIFGAAFLFITSIVLFTQDKSGHIAINYFILALFCTAYALAYRNAYLAYLPAAYL
                     ALTTVYALDAFNVDAWLPALTGLSVIYYLAGVFLRKQEKWGDMFRNSGMALGTILALV
                     ALSATKETGGWYALIIGLLFISETHLRHNGWFEIGVPIHFSIGVFLILHDFKVEQIPY
                     QLLAHSLVWLLSDLLTHLTFKDPRPLRVIVRFIGGALVLVNFAFLFAETDSWIPAIGF
                     GIYTLLFLTISLLYRQPTLLYTFTLTLPLFVAFLFRAFEITQWIHPVIAVAVAYYVLG
                     TTLRVKNRATGWDQSLLYSGLGVGVAVSIGAPILGGLDASIPVAIAATLWAFEAFIKR
                     NAWLAFPANALYLLAYFIILFELNVNEPQFFSVGTALFGLLQHYLLTRAESKSGTFIM
                     GMFSQFVLLGTTYIEMVNKNELGYFFLLFVQSLVILVYGLVIRSRSLTLFPIGFVALG
                     VITVVYSTLKDLGTIFVIGCTGIVLLMLGVGAVLLRERIAKLGEKLSDWKA"
     gene            5139..5543
                     /locus_tag="PLT08_02985"
     CDS             5139..5543
                     /locus_tag="PLT08_02985"
                     /inference="COORDINATES: protein motif:HMM:NF037170.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HNE03458.1"
                     /translation="MNTQNILELRVAITTKDYERLVKFYCLGLGIEPAAVWNNDGGKA
                     LMLNMGNATLEIFDERQAEVVDDLEVGERVSGQIRFALQVPDLNAAMERMLANGATVV
                     HEPVLTPWGDYNVRLQDPDGMQITLFQAKENG"
     gene            complement(5540..6277)
                     /locus_tag="PLT08_02990"
     CDS             complement(5540..6277)
                     /locus_tag="PLT08_02990"
                     /inference="COORDINATES: protein motif:HMM:NF001753.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent deacylase"
                     /protein_id="HNE03459.1"
                     /translation="MNFPADFVSFLTSATRVVVLTGAGVSQESGLRTFRDAQTGLWAR
                     YKPEDLASPEAFSRDPKLVWDWYAWRREAIKGVRPNPGHYALVEMEKRIPQFTLITQN
                     VDGLHRFAGNQNVLELHGNIQKVRCSECRTFTEEWGDDMEGVPTCSVCGGLLRPDVVW
                     FGETLPRAELESAVTASRAAQVYFSIGTSGVVQPAASLAHAARNNGSVVIEINAEPTP
                     LTPKVDFAFHGKSGEILPALIKTVWGN"
BASE COUNT         1607 a         1538 c         1423 g         1821 t
ORIGIN      
        1 tttgattact atcaagtaga cccgctgctt ggcggaaatg aagcactaaa gaaattattg
       61 aatgctgcgc acaagcgaga cattcgggtt gtgttggatg gcgtcttcaa ccatgcttct
      121 cgtgggtttt ggcaatttca ccatgttctt gagacgggcg cagcttcccc ctataaggat
      181 tggttccact ttgaccatga acgtttgaac ggacatcgcc attggggggc gtatccatcg
      241 tctgtggagc agaaacttct gcaacatgaa gacagcctga ctgcgattgg ataccgtgct
      301 tggtggaatt tgcctgcctt gccaaagttc aataccaaca cgcccgctgt ccgagaattt
      361 ttatttgacg tagcagaata ttgggtgaag ttcggcattg acggctggcg cttggacgtt
      421 cccggtgaaa ttgatgacga tgaattctgg cgtgaatttc gtcgccgtgt acgagcagtg
      481 aaccctgatg cttacattgt tggtgagatc tggcatgaag ctcaacgctg gttgcaaggt
      541 gatcaattcg atgctgttat gaattatcta ttcacagcag cctcactgaa tttctttgca
      601 ggggcacatc ttgaccatga agttattcaa catcaaggtg gtatcaaaga ccgtattcat
      661 cgtatgcacg gaaaagcatt tgctgatgaa gtagaacgta ttctcagctt gtacccgcaa
      721 gatattacat tctcgcaact caacttgctc gatagccacg acatgccacg cttcctttcc
      781 tgcgctggcg gcgataaaga ctcgctcaaa ctagcctggc tgttcatctt tactatgcca
      841 ggggcaccct gcatctacta tggagatgag atcggcgtgg acggcgcgca tgacccggac
      901 tgccgtaaat ctttcccttg ggatcaatcc aaatgggaca tgaacttgct tgagtatgcc
      961 aaagcctgta tttctctgcg caagggacag aaagccttac gccgcggcga atacaaacgc
     1021 gtacatgccg aagatgacat gatggctttt gcccgcactt ataaggaaga agccgtagtc
     1081 gtcgccttca acgcatcaaa tacgccaaag tcatttgagt ataagtttga aaaaaatcca
     1141 cgggttcttt ttggaaatcc agaagtttca ggtcagcaca taacggttcc gccccgtagc
     1201 ggagtggtgc ttaagtaata aataagaagc cgggctatag cccggcttct tatttattag
     1261 gacaaatggc gggctagtga attgtccgct ttttcaagtt ttatgcaata ataagggagt
     1321 aaaactttta gtggaaatca aaaatcattt ttatgaacaa gtctaaagaa attcaacttg
     1381 caaaagcctc ttataactgg ctgagcatat ctccaattct gactgttagc acgctattct
     1441 ttgtgtcatc catgggcatt gggaatacag tatgcgataa cagcccgatc atttgcggtt
     1501 atgacaccag cacctatatt agttttgcca ttggcatcgt cttatctgcc ttatggcatc
     1561 tcatcctcct tcaatacaca accaataaag aaagcgagtt tgtccgctta catgggcagc
     1621 gggcattgac tcatgcaggc attcgaactg gcgttgcgct tatcggtcta ggactcgatt
     1681 gggcatctaa tgcgtctgga ggatttgcct gtgtagcagt gcttgtcctt ttgctaatgt
     1741 gggtgattaa cattacgcaa tcgaaacgcc ggatagaaga agacgcaaag ttgttcgatg
     1801 ttccaaacga gcaactagaa gcaccacaaa cagaaactac attttcacaa gaacatgtcc
     1861 atgcggagaa tatggctatg agcggaactg actctcgcaa gccagaagaa gtgttgaacg
     1921 agatctatca aaatctgcaa agcgaggatg atgtggttgt cattcaagcc atctctacat
     1981 tgacaagtct taactacagc agcgaagcca ttcgccgtaa acttgagcag ctatccatcc
     2041 aaagcgacaa taaagatatt cgcgctcaag ccttagccgc actgaattca tctgctaacc
     2101 gtgcagtgca aaagcatttt attgccaaca aattagaccg tggtattcga tatactttgt
     2161 tgcaagaaat caatgattgg gaaaaatcaa acctgctaga caaacaaaat gcagatgtca
     2221 tccgccgccg atacgacttt gactttactc ctgcagcgac aactacatcc tctgcgcctc
     2281 aacctcaggc gatagcagcc cctgcccaac cagcccttgc caccccgcaa gcggctccgc
     2341 cagtaaccac atccacgcca cctgagcctc gcccatctct gttacagacc cttaccagcg
     2401 aagccagcat caaaatttat ctatatcttg gagcgttttt tgtcattgcc gccgctgcca
     2461 tccttggcgc agtggtccca gaattacgtt tgccaattct gatgattggt acctttattt
     2521 tcggtggtct ctccgtcgtc attaaaaaga gacttccgca accaagcttt gctttattca
     2581 ttgttttctc cttcctatta cccatcaccg ctaatagcct tgaagaaagc ctacgtcaag
     2641 ctttcaactt aacagcagat ttcaccgacg cttattgggc gatgattttc attgtgatgg
     2701 caattatttg gattggtagc acttggttgt acgaatctcg cttgttctca ctcacagcat
     2761 ttgggtctct gattctggca agtgtccgca tcggtgacct attcgaagcg gacgctgtgt
     2821 actacaccct tcttatgggg ttaacggctc tcgtaggtct aggtggcacc tggcttctca
     2881 aaaaatggag ggatgaaaaa ttcgcccagc ctctattcct tgcatcccaa cttctccaag
     2941 ctctaaatct agccacgtct cttggcattt tcggagtaaa cacgttccaa tcatctggca
     3001 acaatattct aaatctcatc acacttgcaa catggataac aggtaccctt ttttacctag
     3061 tctctgaatg gctttatcct ttcttcgctt ttccatggct tgctgcggca acttttatcc
     3121 ccatgccgtg gctaatcgcc agtgcatttg accttgaaag tctcggctca tccattgtgc
     3181 tgtttgtttg gggtgccatt ttagcagttg caagcgagcc aatcattcgt tcggagaagc
     3241 tcaaaaagta cggtctgcct attctattag cagccatgcc aagcttggca ttgggaatat
     3301 tcactggctt catctatgaa acctggcttg gtttaacttt ggcactcgga acagccattc
     3361 tctttaccag tctccacatc cttcaaaatc gctggtggct ctggacatta gcgttgctca
     3421 attttgtggc tgcatatttt gcgttcttcc aacttgaggc gattcaaaag atagatatct
     3481 tctttggcta tcagttatta ggcattacca ttctttatct cctcccagat ttattcctaa
     3541 aaaaagattg gatgctcaac ttgcaatggc gtttgccgtt acggattttt ggtgcagcat
     3601 ttctcttcat caccagcatt gtgctattta cccaagataa aagcgggcat attgctatca
     3661 attactttat tcttgcgttg ttctgcacag catatgcgct tgcttatcgc aatgcctatc
     3721 ttgcgtatct acccgccgcg tatttagcgt taaccaccgt atacgcatta gatgctttca
     3781 acgtcgatgc atggttgcct gcattgacgg gtctgtccgt catttattat ctagctggcg
     3841 tcttccttcg caaacaagaa aaatggggcg acatgttccg caatagcggc atggctcttg
     3901 gaaccatctt ggcgcttgta gcgttaagcg ccacaaaaga aactggcggc tggtatgcac
     3961 tgattattgg gctactcttt atttctgaaa cccatttgcg gcacaatggt tggtttgaaa
     4021 ttggcgttcc aattcacttc agcattggcg tgtttctcat cctgcatgat ttcaaggttg
     4081 aacaaatccc ctatcagttg ttggcacatt ctcttgtttg gctcctttca gatttactca
     4141 ctcacttaac attcaaagac cccagaccct tgagagttat cgttcgcttc attggcgggg
     4201 ctctcgtgtt agtcaatttt gctttcctct tcgctgaaac tgactcttgg attcccgcca
     4261 tcggatttgg gatttacacg cttctcttcc tgaccataag cctgctctat cgccaaccaa
     4321 ctttactcta caccttcact ttgacactgc cactttttgt cgctttccta tttcgagctt
     4381 tcgaaatcac ccaatggatt caccccgtca ttgcggtggc agtagcctat tatgtgcttg
     4441 gaacaaccct tagagtaaaa aaccgtgcta caggctggga tcagtccctg ctatatagcg
     4501 gtctcggcgt aggtgtagct gtttcgatag gagcacccat tctcggcggg ttggatgcgt
     4561 cgattccagt tgccattgca gctacacttt gggcattcga agccttcatc aaacgcaatg
     4621 cctggctggc attcccagca aatgctttat atctgctggc atatttcatc atcctctttg
     4681 agttgaatgt gaatgagccg cagttcttct cagtcggaac cgctctattt ggtttactcc
     4741 aacattattt actaacacgc gcagagagca aatctggcac attcattatg ggcatgttct
     4801 ctcaattcgt tctactcgga acaacctata tagaaatggt gaacaaaaat gaacttggct
     4861 atttcttcct gctatttgtt cagtcgcttg taattcttgt ttatggtctt gtcattcgtt
     4921 cacgcagtct aaccttattt ccaattgggt ttgtagcttt aggtgtgatt acagttgtat
     4981 atagcacact aaaagacctt ggaacgattt ttgtaattgg ttgtacaggc attgtcctgc
     5041 ttatgctcgg ggttggcgcc gttctgttac gcgaacgaat tgcgaaacta ggcgaaaagt
     5101 tgagcgactg gaaagcttga tttcaagagg actattaaat gaatacacaa aatattttag
     5161 aacttcgagt tgctattact acaaaagatt atgaaagact cgtgaagttc tattgcctcg
     5221 gactgggaat tgaacctgcc gctgtatgga acaacgacgg cggcaaagct ctcatgctta
     5281 atatgggaaa cgctaccttg gaaatttttg atgaacgtca agccgaagtg gttgatgacc
     5341 tcgaagttgg tgaacgagtg agcgggcaaa ttcgatttgc actgcaagta ccagacttaa
     5401 acgccgccat ggaacggatg ctcgccaatg gtgcgaccgt cgtccatgaa cctgtgttga
     5461 caccatgggg tgactataat gtccggttgc aagacccgga tggtatgcaa atcacgctat
     5521 ttcaggcaaa agagaacggc taattgcccc aaaccgtctt aataagcgca ggcaaaattt
     5581 cgccagactt accatgaaaa gcaaaatcaa ctttgggtgt aagcggggtt ggctcggcgt
     5641 tgatctctat gactacagaa ccattattgc gggctgcatg tgctaaagac gcagcaggtt
     5701 gaacaacacc tgatgtaccg atcgaaaagt aaacttgcgc tgcgcgtgaa gcagtgactg
     5761 ccgattcaag ctctgctctt ggcagggtct ctccaaacca aacaacatct ggtcgaagaa
     5821 gcccgccaca gactgagcag gttggaacgc cttccatatc atccccccac tcttctgtaa
     5881 acgttcgaca ttcggaacaa cgcacctttt gaatattacc gtgtaattcc aatacatttt
     5941 ggtttccagc aaagcgatgc aacccatcca cattttgggt gatgagggtg aattgaggaa
     6001 tccgcttttc catttctact agcgcataat gcccagggtt tgggcgaacg cccttaattg
     6061 cctcacgacg ccaagcatac caatcccaaa ccagctttgg gtcgcgggaa aaagcttcgg
     6121 gggaggctaa atcttctggt ttgtatcgag cccaaagtcc ggtttgagcg tcgcgaaagg
     6181 tgcggagtcc gctttcttgg gagactccgg ctccagtgag tacgacgact cgtgtggcag
     6241 aagtcaaaaa gctgacaaaa tctgcaggga aattcatcta cggttttaca gtggcagatg
     6301 tgggggtggc agtaaagata cattccacca tcttgaggcg ttctttgaaa atatcagtgc
     6361 gccctaattc tgtgccggct tgattcgta
//
LOCUS       DAOQJE010000071         6298 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_6970_length_6298_cov_6.711357, whole genome shotgun sequence.
ACCESSION   DAOQJE010000071 DAOQJE010000000
VERSION     DAOQJE010000071.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 6298)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6298)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6298
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6970_length_6298_cov_6.711357"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..644
                     /locus_tag="PLT08_02995"
     CDS             <1..644
                     /locus_tag="PLT08_02995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE03460.1"
                     /translation="PTETFTPSPIPPTETPSPTATATPFPIVESLKAIVTADKLICRY
                     GPGANYLYLIAFNINTPLRLIGRAPGNNWVLVQNEPQKCWVNSEFIALKEEGDLRSLP
                     SMYPDGYKIPVSPYYGPTTVLTAERNGNDITISWIEIIVSPGKYEDENMFPYIVEVWT
                     CKDGQIVFDTIGSRVPFITFTDESGCSEPSHGRVYIQEKHGYAGPAEIPWPEN"
     gene            complement(694..769)
                     /locus_tag="PLT08_03000"
     tRNA            complement(694..769)
                     /locus_tag="PLT08_03000"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(734..736),aa:Asn,seq:gtt)
     gene            complement(818..1810)
                     /gene="glpX"
                     /locus_tag="PLT08_03005"
     CDS             complement(818..1810)
                     /gene="glpX"
                     /locus_tag="PLT08_03005"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphatase"
                     /protein_id="HNE03461.1"
                     /translation="MEVLGATPTRNLALELARVTEAAAMMAGRFMGRGDKEGADQAAV
                     NAMRLILSTVDMNGVIVIGEGEKDKAPMLYNGEKVGNGSKPDVDVAVDPIDGTRPLAF
                     GRSNSLATVALAPRGTMFDPGPFLYMNKIAVGPEAKGKIDIEKSITENLHAIAKAKGK
                     AVEDLTTIILDRPRHDDMIAEIRKAGARIREIPDGDVAAALMTAWPDAGVDVLLGIGG
                     TPEGVLAACALRAMGGEIQGKLFARDEDELRRGREQGYNFDMVLTMNDLVASEDVFFA
                     ATGITDGELLKGVRYYGDRITTDTLVVRGLTGTVRQITATHKTDKLDNLSSVHY"
     gene            complement(1826..2638)
                     /locus_tag="PLT08_03010"
     CDS             complement(1826..2638)
                     /locus_tag="PLT08_03010"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: protein motif:HMM:NF013327.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004605 - phosphatidate
                     cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0016024 - CDP-diacylglycerol biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HNE03462.1"
                     /translation="MRRRLITALGLAVVGVPAILFGGFLYYLLMGTFLIGAAWEYVHL
                     FRAVKFDPNMIVTVGGVAAVTLTRMFLPQFAQPTLAIVVLLAMAVHLIAYERGRDQSA
                     LDFGVTIGGIVYIGWIGSYLLDLRNLPEGGWWFMLVMFCVWAGDSGAYSIGRAYGNHK
                     MAPRLSPKKSWEGYAASVFTGAHTGALYVWIFQTFGHHIYSITIWQGALMGLFLGALP
                     TLGDLGESMIKRQSGIKDSSDIIPGHGGFFDRIDSWLWGSVIGFYFLTWFIL"
     gene            complement(2631..3377)
                     /locus_tag="PLT08_03015"
     CDS             complement(2631..3377)
                     /locus_tag="PLT08_03015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HNE03463.1"
                     /translation="MTDTPLNIPLEKVPQHVAMIMDGNGRWAIQRGLPRLAGHKAGTE
                     NLRRVIRATVEFGVKYLTIYAFSTENWGRPAEEVNGLMLILQNVIDRELNELHNEGVQ
                     LRHIGRLERLDPAIQKKVLHAIDLTKNNDRLVLNVAFNYGGRDEIVNAIQNIIKDGIP
                     AEEVTDEMVNRYLFTAGVPDPDLIIRTSGELRVSNFLIWQAAYSEWYITPTFWPDFDK
                     EEYHRALATYANRDRRYGKVSSGELQESNA"
     gene            complement(3421..>3710)
                     /locus_tag="PLT08_03020"
     CDS             complement(3421..>3710)
                     /locus_tag="PLT08_03020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ribosome-recycling factor"
                     /protein_id="HNE03464.1"
                     /translation="PNSDGKVIHLNLPPLTEERRRDLVKHMSHRLEESRVAVRNVRRD
                     LHNDIREFEKEKLITEDDLKRGEDDLQKLTDKFIEEIARLGQNKEKEIMEV"
     assembly_gap    3711..3810
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<3811..4145)
                     /locus_tag="PLT08_03025"
     CDS             complement(<3811..4145)
                     /locus_tag="PLT08_03025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HNE03465.1"
                     /translation="MSTDEVKSLLKDAETRMHGAIQSLVDDLNGIRTGRASPALVERL
                     PVEYYGTPTPLQNLASISVPEPRTLTIKPFDAGSLKDIEKAIRSSDLGLNPNSDGKVI
                     HLNLPPLTE"
     gene            complement(4320..5036)
                     /gene="pyrH"
                     /locus_tag="PLT08_03030"
     CDS             complement(4320..5036)
                     /gene="pyrH"
                     /locus_tag="PLT08_03030"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005328724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033862 - UMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HNE03466.1"
                     /translation="MAELKYKRILLKLSGEALGGSSGFGIDVNEAESIASRIKEVYDM
                     GVDVAVVIGAGNLWRGKQGLERGMDRSTADYMGMLATVMNAMALMDALERQGVFTRVL
                     SAIEMRAIAEPYIRRRAVRHLEKRRVVIFGAGTGNPFFSTDTAAALRATEIDAQVVIK
                     ATKVDGVYDSDPKKNPDAKKFDQLSYIDVLNRRLEVMDSTAITLCMENKIPILVVNLW
                     DSKALTSALYGEKVGTLVSG"
     gene            complement(5097..5702)
                     /gene="tsf"
                     /locus_tag="PLT08_03035"
     CDS             complement(5097..5702)
                     /gene="tsf"
                     /locus_tag="PLT08_03035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor Ts"
                     /protein_id="HNE03467.1"
                     /translation="MEITTEMIKQLRGATNAPMLDCRKALQEADGDFDKAVDWLREKG
                     MATAAKRADRDASNGTVELYSHGGGRVGVMVEINCETDFVARNEQFRTLAHEIALQIA
                     ASAPKYIVADEIPAAELEHEAEIARARAKEEGKPDSVLPKIVEGRIEKYKDEVCLLRQ
                     SYIRDESITIEKLILQNVAAIGENIIVRRFQRWELGESINK"
     gene            complement(5745..>6298)
                     /gene="rpsB"
                     /locus_tag="PLT08_03040"
     CDS             complement(5745..>6298)
                     /gene="rpsB"
                     /locus_tag="PLT08_03040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HNE03468.1"
                     /translation="WSTMYQRIQELERLEKLRDSGDINRLTKKEGLLIEREITRLQTR
                     LSGVRAMKRVPDLLFIVDVGREDGAVEEANHLHIPIVALVDTNCNPQNIDYVIPSNDD
                     AIRAIKLLVSKMADAALEGKASRKDEEPEQAAEAQAEAKPKGRPARKVVETDDQVGDD
                     ALLGKSTLAKMAKTDEGTETAAE"
BASE COUNT         1527 a         1593 c         1507 g         1571 t
ORIGIN      
        1 cacccacaga aaccttcaca cccagcccca tccctccaac tgaaacgccc agccctactg
       61 ctacagctac gccattccct atcgttgaaa gcttaaaagc cattgtcact gctgataaac
      121 tcatttgccg ctatggacca ggcgcaaatt atctttatct tattgcattc aacattaaca
      181 cacctttgcg tctgatcggg cgtgcaccgg gaaataattg ggtgctcgtt caaaatgaac
      241 cgcaaaaatg ttgggtcaac tcagaattta tagccctaaa agaagaaggg gatcttcgct
      301 cccttccatc gatgtaccca gatggctata agattcccgt ttcaccatat tatggtccaa
      361 ccacggtgct caccgctgaa cgaaatggca atgacatcac tatctcatgg atagagatca
      421 ttgtcagccc cggcaaatac gaagacgaga acatgttccc ctatatcgtc gaagtatgga
      481 catgcaaaga tggtcagatc gtctttgata ccatcggctc tcgtgtcccc ttcatcactt
      541 tcaccgacga atccggctgc tccgaaccct cgcatggacg ggtttacatc caagaaaagc
      601 acgggtatgc aggtccagca gaaattcctt ggccggaaaa ttagttaaaa tttaaaaata
      661 aaaaagtcct tgagaactca aggacttttt gtatggctcc tggcatagga tttgaaccta
      721 taacctagcg gttaacagcc gctcgctccg ccgattgagc tagccaggaa cgcgagcggt
      781 attatatgca catgccccag gaatgtcaag caatttttta gtaatgaacc gagctcaaat
      841 tatccaattt atcggtcttg tgcgtggctg tgatctggcg gacggtgccg gtcagaccgc
      901 gaactaccaa tgtgtcggtg gtgatacggt cgccgtaata tcgcacacct ttgagcagtt
      961 ccccatctgt gatacctgtg gcagcaaaga aaacatcttc agaagcaaca aggtcattca
     1021 tggttagtac catgtcgaag ttataacctt gttcacgtcc acggcgaagt tcgtcttcat
     1081 cacgggcaaa taatttgccc tgaatttctc cgcccatggc acgcaaggca caagccgcta
     1141 gcacgccttc tggcgtccct ccaataccaa gaagaacatc cacaccagca tcgggccaag
     1201 ccgtcattag agccgcagct acatcgccat cgggaatttc gcgaatacga gcacctgctt
     1261 tacggatctc tgcaatcatg tcatcgtggc gcggacggtc gaggatgatg gtggtcaggt
     1321 cttcaaccgc tttgcctttt gcttttgcga tggcatgcag gttttcagtg atcgattttt
     1381 caatatcaat cttgcctttt gcttcaggtc ccactgcaat cttattcatg taaagaaacg
     1441 gaccggggtc aaacatggtt ccacgcggtg ctaatgccac ggttgcaagc gaattggagc
     1501 gaccaaaagc caaagggcga gtcccatcga tgggatccac tgctacatcc acgtctggtt
     1561 tcgagccgtt gcctactttt tcaccgttgt agagcatcgg agccttatcc ttctcccctt
     1621 ctccgatgac gataactcca ttcatatcca ctgtactgag gatgagccgc atcgcgttga
     1681 ctgcggcttg gtctgcccct tctttatctc cacgtcccat gaagcgacct gccatcatcg
     1741 ccgcggcttc ggtcacacgt gcaagctcga gcgcgaggtt tcgagtaggt gttgcaccga
     1801 gaacttccat gaaatcctcc agagattaga gtatgaacca ggtgagaaaa taaaatccga
     1861 tgacggaacc ccatagccat gagtcgatgc ggtcgaagaa accgccatgc ccggggataa
     1921 tgtcgcttga gtctttgatg cccgattggc gtttgatcat gctttcgcca aggtcaccaa
     1981 gcgttggcaa agcgccgaga aaaagcccca taagcgctcc ctgccagatc gtaatactat
     2041 aaatatgatg tccaaaagtt tggaagatcc acacatagag tgcaccagtg tgtgctcctg
     2101 tgaagacgct ggctgcatag ccttcccaac tttttttcgg gctaaggcgc ggcgccattt
     2161 tgtggtttcc atatgcacgc ccaatggagt aagcaccaga gtcgcctgcc catacacaga
     2221 acatgaccaa cataaaccac caaccaccct ctggtaaatt acgcaggtct aataaatagg
     2281 agccgatcca accgatgtag acaatgccac caatggtcac tccaaaatca agcgcggact
     2341 ggtctcgtcc gcgctcataa gcaatcaagt gaactgccat tgccaataac acgacaatgg
     2401 ctagggtggg ctgtgcaaat tgtggaagga acatgcgagt gagtgtgaca gccgccacgc
     2461 caccaacggt tacgatcata ttggggtcaa atttcacagc cctgaataga tgtacatatt
     2521 cccacgcggc gccgatcaaa aaggtcccca tcaataagta ataaaggaac ccgccaaata
     2581 aaatagcggg cactcctact acggctaatc ccaaagcggt aatgagacgt ctacgcattg
     2641 gattcctgta attcccccga agataccttt ccgtatcgtc tgtcgcgatt ggcataagtt
     2701 gccaacgcac ggtgatattc ttccttgtca aagtctggcc agaacgttgg tgtgatgtac
     2761 cactctgagt atgctgcctg ccagataagg aagttgctga cacgcagttc acccgatgta
     2821 cggataatca aatccgggtc gggcacgccc gctgtaaata agtaacgatt gaccatttca
     2881 tcggtcactt cttcagcagg gatgccatct ttgataatgt tttgaatggc attgacaatt
     2941 tcgtcacgtc cgccatagtt gaatgccaca ttgagcacca agcggtcgtt attcttggtt
     3001 aaatcaatcg catgcaagac tttcttttga atggcagggt caagcctttc aagacgacca
     3061 atatgacgga gttgaacacc ttcattatga agttcgttca attcccgatc aatcacattt
     3121 tgaaggatga gcattaaccc attcacttcc tcagcagggc gcccccaatt ttcggtggag
     3181 aacgcgtaaa tggtgaggta tttcacacca aattcgaccg ttgcgcggat gacgcgacgc
     3241 agattttcag ttcctgcttt atgcccagcg aggcggggaa gtccacgttg aattgcccaa
     3301 cgtccgttgc catccataat catggcaaca tgttgtggga ctttttcaag cggaatgttc
     3361 aaaggggtat ctgtcatatg gatcgacctt ttcaatgcca aaaggcatca aaggggatgg
     3421 ctatacttcc atgatctctt tttctttgtt ttgaccaagt ctggcgatct cttcgatgaa
     3481 tttatcggta agcttttgaa ggtcgtcttc accgcgtttg aggtcgtctt cagtgataag
     3541 cttttccttc tcaaattcgc ggatatcgtt atgcagatca cggcgcacat tgcgaactgc
     3601 cacccgagac tcttcaaggc ggtgactcat gtgcttaacc aaatcgcggc ggcgttcttc
     3661 tgtaaggggc ggcaggttca agtgaatgac cttaccatcg gagttgggat nnnnnnnnnn
     3721 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3781 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn tcttctgtaa ggggcggcag gttcaagtga
     3841 atgaccttac catcggagtt gggattcagc ccaaggtctg aggagcgaat cgctttttca
     3901 atatccttga gagagccggc atcaaaaggc ttaatggtca atgtgcgtgg ttcgggcaca
     3961 ctgatggaag ctaaattttg aagcggggtt ggggtgccat agtattcgac cggaagtctt
     4021 tccaccaatg ccgggctagc gcgcccagta cgtatgccat tcaggtcgtc aacaagggat
     4081 tggatcgcgc cgtgcatgcg ggtttcggcg tcttttaaca aactctttac ttcgtcagta
     4141 ctcacgatct tcctccaaga aaataattac aatacaagga taccttaaaa caagaattta
     4201 ggctatggga tggtaaaaac attgatagtt tatgaaaaat atagagagac tatcaactat
     4261 tggaacagaa aagcctctcc ccttccgttg gaaagagaga ggcttgggta taaaaagaat
     4321 tacccagaga ccaaagtgcc cactttttcg ccatataggg cgctggtaag cgcttttgaa
     4381 tcccacaagt tgaccacaag aatcggaatt ttgttttcca tacacagagt gatggcagtg
     4441 ctatccatca cttcgagacg gcggttgaga acatcgatgt agctgagttg gtcaaacttt
     4501 ttcgcatcgg ggtttttctt cgggtctgag tcatacaccc cgtccacttt ggtggctttg
     4561 atgacaacct gcgcatcgat ttcagtagcg cgtaacgccg cagcggtatc ggttgagaag
     4621 aaaggatttc ccgttccagc gccaaaaatg acgacacggc gtttttcgag gtggcgaaca
     4681 gcgcgtcggc gaatgtaagg ttcggcgatg gctctcattt caatggcaga gaggacgcgg
     4741 gtaaacacgc cctgtcgttc gagggcatcc atcaacgcca tggcattcat cacggtggca
     4801 agcatgccca tatagtctgc tgtggagcgg tccatgccgc gttcaagccc ttgcttgccg
     4861 cgccaaaggt tcccagcgcc gatcacaact gctacatcta cgcccatgtc gtagacttct
     4921 ttgatgcggc tcgcaattga ctctgcttcg ttaacatcaa tgccaaatcc ggatgaaccg
     4981 ccaagcgctt cgccacttaa cttgagcagg attctcttat atttcaattc agccattctc
     5041 atacctccaa atcgaattag aaaaaaagcc aacccgaagg ctggcttttt aagaaactat
     5101 ttgttgatgc tttcgccaag ttcccaacgc tggaagcgtc ggacgatgat attttcacca
     5161 atagcagcca cattctggag aatgagtttt tcaatggtga tcgattcatc gcggatgtat
     5221 gactggcgca aaaggcagac ttcgtctttg tacttttcga tgcggccttc cacgatcttg
     5281 gggagaacgc tatcgggttt gccttcttct ttggcacgag cacgggcgat ctcggcttcg
     5341 tgttcgagtt cggcggcggg aatttcatcc gcaacaatgt atttcggagc gctagcagcg
     5401 atctgaagag caatttcatg ggcaagggtg cggaactgtt cattgcgagc aacgaagtcg
     5461 gtttcgcagt tgatttcaac catcacgccc acgcgtccgc ccccgtggga atacagttca
     5521 acggtgccgt tggaggcatc gcggtcggcg cgcttggcag cggtcgccat gcccttttcg
     5581 cggagccagt ctacagcctt atcaaaatcg ccatcagctt cctgaagcgc cttgcggcaa
     5641 tccaacatgg gagcgttggt tgcgccgcgc agttgcttga tcatctcagt cgtaatttcc
     5701 atcgtgtcta tccttgttgc tttctgtgtt attcaactag cagcttattc agcagcggtt
     5761 tcagtgcctt cgtcggtctt tgccatcttg gcgagagtgg acttacccaa gagggcatca
     5821 tcacccacct ggtcatcggt ctcaaccact ttgcgagcag ggcgtccctt gggcttggct
     5881 tcagcctgag cttcggcagc ttgttcgggt tcttcatcct tgcgagaggc tttgccttcg
     5941 agagcagcat ctgccatctt ggagacaagc aacttgatgg cacggatggc atcatcgttg
     6001 gaagggatga cgtagtcaat gttctgggga ttgcagttgg tatcgaccaa agccacgatc
     6061 gggatatgca aatgattggc ttcttctaca gcgccatctt cgcggcctac gtcgacaatg
     6121 aagagcaggt cgggcacacg cttcatggcg cgaacaccgc tgaggcgggt ctggaggcgg
     6181 gtgatctcgc gttcgatcaa caagccttcc ttcttggtga ggcggttgat gtcgccacta
     6241 tcgcgcagtt tttcgaggcg ttcgagttcc tgaatacgct gatacatggt agaccagt
//
LOCUS       DAOQJE010000072         6298 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_6973_length_6298_cov_5.120615, whole genome shotgun sequence.
ACCESSION   DAOQJE010000072 DAOQJE010000000
VERSION     DAOQJE010000072.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 6298)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6298)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6298
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6973_length_6298_cov_5.120615"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            236..1141
                     /locus_tag="PLT08_03045"
     CDS             236..1141
                     /locus_tag="PLT08_03045"
                     /inference="COORDINATES: protein motif:HMM:NF037627.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HNE03469.1"
                     /translation="MDRKEFGILVSHLRQDLNLTQFELAENAEVDIAVISQIERGVKK
                     HLDSELLFKLANSFHLTTIERREFFLAACGLGETRIIRQPSAALATDTINAEKVLGKM
                     VKIVEKLSIPAFIMDVYSDVIAINYIAAAFFQVTPDVLDVASKVPGGYSTVRMMFGKD
                     LHLRSKVLENWDQYAVGSMRFVRENSLRYRAKPYFQYLMKSFQDRNEFPLFERYWRMV
                     SSFEQDREANVDVFSYTHADYGPLNYATATTVSITPHGELFLNQYLPLDAHTRNLFSA
                     LEQQTGQGVVTELASWPEKKIPMNT"
     gene            1241..1732
                     /locus_tag="PLT08_03050"
     CDS             1241..1732
                     /locus_tag="PLT08_03050"
                     /inference="COORDINATES: protein motif:HMM:NF024773.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HNE03470.1"
                     /translation="MTKLDSMVQNFLAQKRIAVVGVSDKRETGCNAAYQKFKDAGYEV
                     FAVNPRIASFQGDACYPDLAALPTKPDAVFILANPKVTDQIVSQCVDLGVKHVWMHCM
                     MGTKPGLATSTTSVSPTAIDVCKANGISVIPGSCPNQYLNPDGGHKFMNGMFKLFGFM
                     SVN"
     gene            1808..3277
                     /locus_tag="PLT08_03055"
     CDS             1808..3277
                     /locus_tag="PLT08_03055"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HNE03471.1"
                     /translation="MTPNSASPLIEIKNLVKTYKTPAGDFTAVKGIDVEVQRGEFVAV
                     IGKSGSGKSTFINMITGIDRPTSGEIYIDGAPVHSFSEAQMAAWRGRNLGIVFQFFQL
                     FPTLTLLENVILPMELNHLYTKKERRERAMDLLKLVEMDHQAHKLPTTISGGQQQRVA
                     IARSLANDPPLLIADEPTGNLDSKTAAKIFSLFEELVASGKTILMVTHDSDLARRVNR
                     TILISDGEVVNEYLVRALATLTQDQLVEVARRVKPQIFPPDASITRQGEVGDKFYILL
                     DGKADVYLNEAGGGQVLVNELKRGQYFGEMALLGSGVRTATVKASPETSVSVAALDSM
                     AFNKLVKESRTLQDELKSIIERRNILDQLKVFASAMVNDPAGKLPIEFGANQEIIKQG
                     DGGEIFYLILEGKVNVIVDDSQGEETIIEHLGKGAIFGESILLGDTRYRTTVRVSKEK
                     PAKVLAITPEEFNQFAEQNKQAEAKKKPTESSIRRKVRK"
     gene            3274..5613
                     /locus_tag="PLT08_03060"
     CDS             3274..5613
                     /locus_tag="PLT08_03060"
                     /inference="COORDINATES: protein motif:HMM:NF014717.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HNE03472.1"
                     /translation="MSVQTQKILRDLWVNKSRSLLITLAVAIGVAAFGLMISGRIVLE
                     ENLRDVYAATKPAHAILSLSPFDQTLLADVRNMDDVVAAQAYRADLARLKTGPDSWLG
                     FEIQTIPDFGNIAVNKITAEGELSNPPLNSIFLERSLKNVIDIGDTVTIRLLNGNEYT
                     LTVAGFVNDLSRLPAEISLAGIGYITPETANELELEPEYNQLLIRYADGSTRTEIEKL
                     TTKVTEAIEEDEYIVFNANVPVPDTYILGDNMTSVLFILNSLGFLTLILSAFLVTSVM
                     SAMMSQQIPQIGILKSLGAHVGQTMSIYFREVLLFGIFALILAIPMGLVGAYFLADGL
                     ASSMNFDVPNFYLPTQTLILQAISALLAPLLAALIPIISGSRITIREALSNYTPDSAS
                     NALSLGEIPQIANLSIRNTFRRKSRLALTFAALLLAGSMFISIIGIREEMRQASIEMQ
                     KEANYDLDVDLARPYSQSTLEETALRIKGVKEAETWAVDTGRLVFNKDHLSGSIILYG
                     IPQGTTMTQPAVINGTWLSKPKSIFVNNDFLLLSPDLKVGSVITLNIGGKEEEWTIVG
                     STGRGFIPIAYVYYDDLTNVTGLDGFASRLVVRTDKSDSAFQTSAQSDVLSHLAAEDI
                     DVVNSSTTTERNETSLEQMDTLIILLMAMVILIAIVGGLGLAITMSLNVMERTREIGI
                     LRSLGAQNGVVRRVVMIEGLVIGVISWAISIPCSVPLSIFLGNSLGLSLLARPLDYIF
                     SVPAMLIWLALTVVIAIIASILPAQSAAHLTIRDALVYE"
     gene            complement(5653..6021)
                     /locus_tag="PLT08_03065"
     CDS             complement(5653..6021)
                     /locus_tag="PLT08_03065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE03473.1"
                     /translation="MPTRSEFPIVFEHLKSILVSYAKKMTVKNDISGLYYLDAGYSEK
                     WKRDLFFASVQIKKSYVSFYLMPVYMYPDLLKNISPELKKHMQGKSCFNFKKIEKSLF
                     EELSQLTKQGHERMMKEEFS"
     gene            complement(6045..>6298)
                     /locus_tag="PLT08_03070"
     CDS             complement(6045..>6298)
                     /locus_tag="PLT08_03070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE03474.1"
                     /translation="RELNVQVVHVGVAWFRALNKYPSLQLWQEDGSHPSEQGTYLAAS
                     VFYVTLFKESPVGLNYHANLTEEVASQLQSVAESILTNP"
BASE COUNT         1658 a         1686 c         1379 g         1575 t
ORIGIN      
        1 cctatttata gccgcaaatc gtaaaaattt cgtaagagac ttacttaaaa taaaacctca
       61 gccttaaggc tgaggtccca aatgtagttc ccctccccca ctacatgagg cagggttgca
      121 agaacggaaa caaacaactc aaatattgta cgattttggg ttgaaaagtc cagcaaaaga
      181 gtttaaactt gactcgaaaa aatttttgag tcgaatttat ttggtaggag ccgtcatgga
      241 ccgcaaagag tttggaatcc tcgtttctca tcttcggcaa gacctcaact taacgcagtt
      301 tgaactggca gaaaatgcag aagtggatat cgcagtcatc agtcaaattg aacgaggcgt
      361 gaaaaaacat cttgattcag aattgctttt caaactcgcc aattcgtttc accttactac
      421 cattgaacgc cgtgagttct tccttgctgc ttgcgggctc ggcgaaactc gcattatccg
      481 ccaaccctct gccgcgctcg caaccgatac gatcaatgcc gaaaaagtcc tcggcaaaat
      541 ggtcaaaatt gttgaaaagc taagcatccc cgcatttatc atggatgtct attcagatgt
      601 gattgccatc aactatatcg ccgctgcttt ctttcaagtt acacctgacg ttctggatgt
      661 cgcatcgaaa gtccccggtg gatatagcac cgtccgcatg atgtttggaa aagacctgca
      721 ccttcgttcc aaagtgcttg aaaactggga ccagtacgcc gtcggctcga tgcgcttcgt
      781 ccgcgagaac agcttgcgat accgtgccaa accttatttt caatatctca tgaagtcatt
      841 ccaagaccga aatgaatttc cgctcttcga acgctattgg cgcatggtct cgtcctttga
      901 acaagaccgt gaagccaatg tagatgtctt cagttacacc catgctgatt acggtccgct
      961 caactatgca accgctacta ccgtctcgat caccccacat ggcgaattat tcctcaacca
     1021 atatctgcca ctcgatgccc acacccgtaa tcttttttct gcacttgagc aacaaacagg
     1081 gcaaggtgtt gtcacagagc ttgcctcatg gcccgaaaag aaaatcccta tgaacacctg
     1141 atattcgtca tattgaaggc aggaatgtcc tccttataat cattaatagc gccacgcttc
     1201 tgtaacaaga agcgtaagca atatgaataa ggagaatgtc atgacgaaac tcgatagtat
     1261 ggttcagaat ttcctcgccc aaaaacggat cgccgttgta ggcgtttcag acaagcgcga
     1321 aaccggatgc aatgccgcct accaaaaatt caaggatgca ggctacgaag tcttcgctgt
     1381 caacccgcgc atcgcatcgt tccaaggcga cgcctgttac cccgacctag ccgctctgcc
     1441 caccaagccc gatgcggtct tcatcctcgc caaccccaaa gtgaccgacc agatcgtcag
     1501 tcaatgcgtg gacttgggcg tgaaacatgt gtggatgcac tgcatgatgg gcaccaagcc
     1561 cggtctcgcc accagcacaa ccagtgtatc ccccaccgca atagatgtat gcaaagccaa
     1621 tggcatctcc gtcatcccag gctcttgccc taatcaatac ctcaaccccg atggcgggca
     1681 taaattcatg aatggaatgt tcaagctatt tggttttatg agcgtgaact gactgcccac
     1741 caactttctt gaacagtcat ggcaaaacca tggctgtttt tttattaatt cgattaaact
     1801 tgacaacatg actccaaatt ccgcctcccc attgatcgag atcaaaaact tagtgaaaac
     1861 ctataagacc cccgctggag atttcactgc cgttaagggc attgacgtgg aagtgcaacg
     1921 gggagaattc gttgccgtca ttggcaaatc gggcagcgga aaatctactt tcattaatat
     1981 gatcaccggc atagaccgcc ccacctcggg cgaaatctat atcgatggtg cgccagtcca
     2041 ctcttttagt gaggcgcaaa tggcagcctg gcgcggacgg aatcttggga tcgtctttca
     2101 attctttcag cttttcccca cattaaccct gttagagaac gtcatcctcc caatggaatt
     2161 gaaccatctc tataccaaaa aagaacgccg tgaacgcgcc atggacctgc tcaaactggt
     2221 cgaaatggac catcaggctc acaaacttcc caccactatt tcaggcgggc agcaacaacg
     2281 tgtggcaatc gcccgctccc ttgccaatga cccaccactg ctcattgcag acgaacccac
     2341 cggcaacctc gactcaaaaa ctgccgccaa gatattttct ttatttgaag aactcgttgc
     2401 ttccggtaaa accattctga tggtcacaca cgatagtgac cttgcccgcc gcgtcaaccg
     2461 caccattctt atctcagatg gtgaagttgt aaacgaatat cttgtgcgtg ctctggcaac
     2521 tctcacccaa gaccaactcg tggaagtggc aaggcgcgtt aaaccacaaa tcttccctcc
     2581 tgatgccagc attacccgcc aaggcgaagt cggcgataag ttctacatct tgctcgatgg
     2641 taaagcagac gtgtacctca acgaagcagg cggtggtcaa gttctggtga atgaactcaa
     2701 acgcggacag tacttcggcg aaatggcatt actcggaagt ggcgtacgaa ctgccaccgt
     2761 caaagcctcg ccagaaactt ccgtttctgt cgccgcattg gattcaatgg cattcaataa
     2821 actagttaaa gagtcacgca ctctgcaaga cgaactcaaa agcattatcg aacgccgcaa
     2881 tattcttgac caattaaagg tctttgcctc tgccatggtt aatgaccctg cgggtaaatt
     2941 acccatcgaa ttcggagcca atcaggagat catcaagcaa ggagatggcg gtgaaatctt
     3001 ctatctgatt cttgaaggca aagtaaatgt gatcgtggac gattctcaag gagaagagac
     3061 catcatcgaa catcttggta aaggtgcaat ctttggcgaa tcgatcctgc ttggtgacac
     3121 acgctatcga accactgtcc gcgttagcaa agagaagcca gccaaagtcc ttgcgatcac
     3181 gccagaagaa ttcaatcaat ttgcagaaca aaacaaacag gctgaagcga agaaaaagcc
     3241 cactgaaagc agcatcaggc gaaaggtccg caaatgagcg tgcagactca aaaaatcctg
     3301 cgtgacctgt gggtaaacaa gtcacgcagt ttgcttatca cgctggcggt cgccattggg
     3361 gtggcagcct ttgggcttat gattagcggt cgcattgttt tggaagaaaa cttacgcgat
     3421 gtgtatgccg ccacgaaacc agcgcatgcc attctttcgc tctcgccatt tgaccaaacg
     3481 ttgctcgcgg atgtgcgcaa catggatgat gttgttgcgg cacaagccta tcgtgcagac
     3541 ctcgcccgcc tcaaaaccgg accagactcc tggcttggct tcgagatcca aactattcct
     3601 gatttcggca atatcgcggt taataaaatt actgccgagg gtgaactttc caacccgccg
     3661 ctgaactcca tctttctgga acgttcacta aaaaatgtga tcgacattgg cgatacagtc
     3721 acgatccgtt tgttgaacgg aaatgaatac accctgaccg ttgccggttt tgtaaatgac
     3781 ctttctcgtt tacctgctga gatcagcctg gcagggattg gttacatcac cccagaaaca
     3841 gccaacgaac ttgaactcga gccggaatac aatcagctgc tcatccgata tgcagatggc
     3901 tctacgcgca ccgaaattga aaaactgacg accaaagtga ccgaagccat cgaagaggat
     3961 gagtacatcg ttttcaacgc gaatgtaccc gtgcccgata cttacatcct cggcgataac
     4021 atgacatcgg ttcttttcat tttgaattcg cttggcttcc tcacactaat cctgagcgcg
     4081 ttcctcgtca ccagtgtcat gtctgccatg atgtcgcagc agatccctca gatcggtatt
     4141 ctaaaatctt taggcgcgca tgtcggtcag accatgtcca tctattttcg agaagtgctg
     4201 ctgttcggca tcttcgcctt gatcctcgcc attccaatgg gcttggtcgg cgcttatttc
     4261 cttgcagatg gtctggcttc gagcatgaac ttcgatgtgc cgaatttcta cctgcccact
     4321 caaaccctga tcttgcaagc catcagtgca ttgcttgcgc cattattggc ggcgctcatc
     4381 cctatcattt ctggttcgcg catcacgatc cgtgaagcgc tcagcaacta cactcccgat
     4441 agtgcctcca atgcattaag ccttggagag attccgcaaa tcgcaaacct ttcgattcgt
     4501 aataccttcc gccgcaaaag ccgccttgca ctcaccttcg ccgcactgct cctggctggc
     4561 tccatgttca tttccattat cggcattcgc gaagaaatgc ggcaagccag tattgaaatg
     4621 caaaaagaag cgaactacga tctcgatgta gacttggcac gcccatattc tcaaagcacg
     4681 ttggaagaaa ctgccttgcg tataaaaggt gtaaaagaag ctgaaacctg ggcagtggat
     4741 acaggtcggc tcgttttcaa taaagaccac ctgagcggca gcatcatttt gtatggcatt
     4801 ccacaaggca caaccatgac ccaacccgca gtcatcaacg gcacatggtt atccaaaccc
     4861 aaaagcatct tcgtcaacaa cgattttctt ttgctctccc ccgatctaaa agttggctct
     4921 gtcattacgc tgaacattgg cggcaaagaa gaagagtgga ccatcgttgg ctccaccggg
     4981 cgcggattta tccccatcgc ttatgtgtac tacgatgact tgaccaacgt taccgggctg
     5041 gatggcttcg ccagccgcct cgtagtgcgg accgataaat ctgattcagc ctttcaaacc
     5101 tcagctcaat ctgacgttct ctcccaccta gccgctgaag atatagatgt tgtcaattca
     5161 tccaccacca ccgagcgaaa tgaaacctca ctcgaacaga tggataccct gatcatcctt
     5221 ctgatggcaa tggtgattct gatcgccatc gtcggcggac tgggacttgc catcaccatg
     5281 agtctcaacg tgatggaacg cacccgcgag atcggtatat tgcgttcact gggcgctcaa
     5341 aatggagtcg ttcgccgggt cgtgatgatc gaaggcttgg tgatcggtgt aatcagttgg
     5401 gcgatctcca tcccctgctc agttcccctc tctatatttt tgggcaattc cctcgggctt
     5461 tcgctgcttg cccgcccatt ggattacatc ttctccgtcc ctgccatgct gatctggctc
     5521 gccctaaccg tggtgatcgc catcattgcc agcatcctgc ctgcgcaaag cgccgcccac
     5581 cttacaattc gagacgccct ggtgtatgaa taaagttcag ggacagcaac aagcatatcg
     5641 tccctaaaac aattacgaaa attcttcttt catcatgcgt tcatgtccct gtttggtgag
     5701 ttgacttaac tcctcgaaca gggatttttc gatcttcttg aaattgaaac aggattttcc
     5761 ctgcatgtgt tttttgagct ctggcgagat gtttttcaac aaatcggggt acatgtaaac
     5821 cggcatgaga tagaacgaga cataactctt cttgatctgt accgaggcga agaaaaggtc
     5881 gcgcttccac ttttcgctgt agcccgcgtc taaataatac agtccactta tatcattctt
     5941 aacagtcatc tttttggcat aggatacgag aatggatttg aggtgttcaa atacaatcgg
     6001 gaattctgat cttgtaggca tgagttctct cctatattgc tactttatgg gttggtcaga
     6061 atactttcag ccacagattg caattgacta gcgacttctt ccgtaaggtt tgcatggtag
     6121 ttcaaaccta cggggctttc tttgaaaagg gtgacataga aaacactagc agcaagatag
     6181 gtgccttgct cagaagggtg actaccatct tcctgccaaa gctgtaggct aggatattta
     6241 ttaagcgccc gaaaccatgc cactcccaca tgcacgacct gtacgttaag ctcacgtg
//
LOCUS       DAOQJE010000073         6290 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_6984_length_6290_cov_6.783480, whole genome shotgun sequence.
ACCESSION   DAOQJE010000073 DAOQJE010000000
VERSION     DAOQJE010000073.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 6290)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6290)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6290
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6984_length_6290_cov_6.783480"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..594)
                     /locus_tag="PLT08_03075"
     CDS             complement(<1..594)
                     /locus_tag="PLT08_03075"
                     /inference="COORDINATES: protein motif:HMM:NF024867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNE03475.1"
                     /translation="MLTELHIQNFAIIDKLDLRFGPGLIILTGETGAGKSIILDAVVM
                     LIGGKADTTFVRSESDAAFVEGVFQLKGPEREAVHEVLNREELMDDPDYVVLMREVRK
                     EGRSVARINGRTVNVGLLKELGALLIDIHGQAEHLSLLDPRAHLGLLDRYADVAKPLN
                     DYRQTYHSLLTLRHELNDLRKAQADSDRRIEMLTYQVE"
     gene            complement(598..1122)
                     /locus_tag="PLT08_03080"
     CDS             complement(598..1122)
                     /locus_tag="PLT08_03080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE03476.1"
                     /translation="MTEFSTTTTCYVHPNRETSLRCKRCERYICTSCAVSTPTGYICK
                     ECMRERQKSFDTALWYDFALGFVVAGLLSGVASFLVTLIGAIGFIGFMLVFIGSSAAG
                     GIIAEAVRAVTRRHRSRALFMTVGAGVIAGALPMLLVQLLSVNVFGLIFQGIYLVIAV
                     PLVYARLSGIQLTR"
     gene            complement(1119..1982)
                     /locus_tag="PLT08_03085"
     CDS             complement(1119..1982)
                     /locus_tag="PLT08_03085"
                     /inference="COORDINATES: protein motif:HMM:NF039482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+)/NADH kinase"
                     /protein_id="HNE03477.1"
                     /translation="MSSPFIPKRIVVTAYPKMPEAFTEAAAMSVFLKEKGIDAPVGSL
                     YDEELRKRVKQGEFDMLIAVGGDGSVLRAGHLCAPSGVPILGVNLGRLGFLIQIDRKE
                     WRDYFMRLLAGEAWIENRMMLRAEHIRAGEKLGSSLALNEVVVGRGQNLRPVRLTAYV
                     DKRELTSYVADGLIAATATGSTAYALAAGGPILPPELRNILLVPIAPHLSVDRAVVLS
                     EGTSVDIVMKSENAIFSVDGQPPTPLMEEDHVNVTATDVTAQFVRFGNPGYFYRNITM
                     HINENSSGFPR"
     gene            complement(2020..2532)
                     /gene="cas4"
                     /locus_tag="PLT08_03090"
     CDS             complement(2020..2532)
                     /gene="cas4"
                     /locus_tag="PLT08_03090"
                     /inference="COORDINATES: protein motif:HMM:TIGR00372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated protein Cas4"
                     /protein_id="HNE03478.1"
                     /translation="MTSIVLILFLLALIFFWQSNRQQKAAGLPGGRVIYTDTRGWGKV
                     EKPLFYAALGLTGKPDYLIEKNGQIIPVEVKSGRAPEAPYDSHIFQLASYCLLVEKTY
                     GRRPPYGIIHYENRDFAIDYTRELETALIELIADMREDDVRRDVARSHEQASRCKRCG
                     YHDICEQRLA"
     gene            complement(2529..2765)
                     /locus_tag="PLT08_03095"
     CDS             complement(2529..2765)
                     /locus_tag="PLT08_03095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE03479.1"
                     /translation="MRPIRSSEIGSYLYCRRAWWYRKIGTPSENKAELAAGTELHRKH
                     GRQVLASALTRTLGWLLLLAAILTLVAYLAFQVL"
     gene            2864..3415
                     /locus_tag="PLT08_03100"
     CDS             2864..3415
                     /locus_tag="PLT08_03100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyltransferase family protein"
                     /protein_id="HNE03480.1"
                     /translation="MTKHDTYSIDIAGVKRELRLFEIKPGLRIAILNILGDTELVQAC
                     ARGLADKVKNVEYDIIVTAEAKSIPIAHALSVETKKPYVVLRKTYKPYMGDALKAETL
                     SITTGQPQVLILDEKDRELIQGKKVLVVDDVISTGSTLQGMRMILDKANATVAGEAAI
                     LTEGDRSQWMNIHSLGHLPLFTD"
     gene            complement(3444..3686)
                     /locus_tag="PLT08_03105"
     CDS             complement(3444..3686)
                     /locus_tag="PLT08_03105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000377063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HNE03481.1"
                     /translation="MSCFCYILECADGTFYTGWTTDPERRVSQHNKGVGARYTRTRRP
                     VRLVYLEEQPDKITALKRERAIKALSRKKKMELFMM"
     gene            complement(3683..4672)
                     /locus_tag="PLT08_03110"
     CDS             complement(3683..4672)
                     /locus_tag="PLT08_03110"
                     /inference="COORDINATES: protein motif:HMM:NF014234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase"
                     /protein_id="HNE03482.1"
                     /translation="MPVKITIIGLGQIGASIGLALAAHKDRVVTTGHDKEYSVEQRAK
                     KLGVVNETNHNLPSSVENADLVILALPLNEIRETLGHIAQDLRQDVVVVETAPVKAEV
                     TKWVKEILPETTHYVGVVPAIGPAYLDVTASGLDSAKADLFERGIFLVSAPSGTSGEA
                     VKLVTDFVKLLGSTSILTDVVESDGLMASSYILPRLASVALLNGTMSQPGWNEVKKSA
                     SRGYFAATSALLDNSLEGLAMLAEHNRANTIHSLNVMINSLLDLRDDLENEDHASFVK
                     RLKSAKEGRESWLVEREKADWSRIPGEEIEKPSFMESLFGSKLGKLGKDKGKG"
     gene            4909..5433
                     /locus_tag="PLT08_03115"
     CDS             4909..5433
                     /locus_tag="PLT08_03115"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: protein motif:HMM:NF013222.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HNE03483.1"
                     /translation="MTLTPSNLYLGELNDTPLGDFRLVASDLGLIAVEWANNQSELDA
                     YLRKIKRSCEPNQRKIAPYLKELREYLNGKRTTFTIPIDWTPFTPFQREALQAVFRVP
                     YGETRTYIDIAREIDHPHAYRAVGAANAMNPMPIIVPCHRILGTDGKLHGYGGGDGLP
                     TKEWLLKFEGVTMA"
     gene            5447..5968
                     /locus_tag="PLT08_03120"
     CDS             5447..5968
                     /locus_tag="PLT08_03120"
                     /inference="COORDINATES: protein motif:HMM:NF014471.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015109 - chromate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015703 - chromate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromate transporter"
                     /protein_id="HNE03484.1"
                     /translation="MDIHLQLFWTFLKVNLLSTSGPASVGLLYHEAVGRFVTEAQFVQ
                     AVGFSSVLPGSDALQLAMYIGYAAGGITGGLVALIASILPPTVIILGVTMILHRLRRE
                     AWVNSFVEGLTPAIAVLMVFTAWKIFEKGGGMSWFGWGIALTSFLAMWRKIPERFVII
                     IAGIIGVIFLSGS"
     gene            5965..>6290
                     /locus_tag="PLT08_03125"
     CDS             5965..>6290
                     /locus_tag="PLT08_03125"
                     /inference="COORDINATES: protein motif:HMM:NF014471.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015109 - chromate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015703 - chromate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromate transporter"
                     /protein_id="HNE03485.1"
                     /translation="MNTSNPSKYSQLFWMFLKVNLLSPSGPASVGLLYKEAVGTLMTE
                     ERFVEAVGFSNFLPGSEALKLAMFVGYAAAGVPGVFTALLGAMLPPTVMMFIVAFILQ
                     QVQHED"
BASE COUNT         1515 a         1549 c         1659 g         1567 t
ORIGIN      
        1 ctctacttga taggtgagca tttcaatgcg gcggtctgag tcggcttggg ctttgcgcag
       61 gtcattcagc tcatggcgca gggtcaacaa cgagtgatag gtttggcgat agtcgttcaa
      121 tggcttggct acatcggcat agcggtcgag caagccaaga tgcgcgcgtg ggtcgagcag
      181 ggagagatgt tctgcttgcc catgaatatc gatcaggagc gcgcccaatt ctttgagcaa
      241 gcctacattc accgtccgcc cgttgatgcg tgccacgctg cgtccttctt tgcggacttc
      301 acgcatgagc acaacataat cgggatcatc catcaattct tcgcggttga ggacttcatg
      361 caccgcctca cgttctggac ctttgagctg gaacacgcct tcaacaaatg cggcgtctga
      421 ttcagagcgc acgaaggtcg tgtcggcttt gccgccaatg agcatgacca cagcatcaag
      481 gatgatggat ttgcccgcgc cggtttcacc ggtgagaatg atcaaacccg gaccaaaacg
      541 caggtcgagt ttgtcgatga tcgcaaaatt ttggatgtgg agttctgtga gcataggtta
      601 tctagtgagt tgaataccgg aaaggcgggc gtagactaaa ggaacagcaa taacaagata
      661 aatgccttgg aagatcaacc caaagacatt gacgctcaaa agttgtacaa gaagcatggg
      721 caaggcaccc gcaatcacac cagcgccaac tgtcatgaaa agcgcgcgcg agcggtgtcg
      781 gcgagtgaca gctcgaacgg cttcagcgat tatgcctccg gcggcggaag agccgatgaa
      841 aacaagcatg aaaccgatga agccaatcgc accgatcaag gtcactaaga aggatgcaac
      901 gccagagagg agtccggcaa cgacaaagcc caaggcaaaa tcataccaaa gagcggtgtc
      961 gaaagacttt tgcctttcgc gcatgcactc tttgcaaata tagcccgtag gggtgctgac
     1021 ggcacatgag gtgcagatgt aacgctcaca gcgtttgcaa cgcagagagg tttctctgtt
     1081 cgggtgaaca taacaagtag ttgtggtgga gaattcggtc atcgtggaaa tcctgaagaa
     1141 ttttcgttta tgtgcatggt gatatttctg taaaagtatc ctgggtttcc aaatcgtaca
     1201 aattgtgcgg ttacatcggt cgccgtgacg ttgacatggt cttcttccat taagggtgtg
     1261 ggtggctgac catccacact gaaaatggcg ttctcacttt tcattacgat atctacagat
     1321 gtgccttctg aaagaacgac agctcggtct acagaaagat gcggcgcaat gggaaccaat
     1381 aaaatattgc gcaattcggg cggcaagatc ggtccgcctg cggcgagggc ataggctgtg
     1441 gagccggttg ccgttgctgc aattaaacca tcggcgacat aactggtgag ctcgcgttta
     1501 tcgacataag cggtcaaacg caccgggcgg aggttttgtc cgcgcccgac gacgacttca
     1561 ttcaacgcca gtgacgaacc aagtttctca ccagcacgga tgtgttcagc gcgcaacatc
     1621 atacgatttt cgatccatgc ttcgccagcg agcagccgca taaaataatc gcgccattct
     1681 ttgcggtcga tctgaatgag gaacccgagc cgacccagat taacacccaa gatcggaacg
     1741 cccgatgggg cacacaaatg cccggctcgc aagacgctgc catcgccgcc aacggcgatc
     1801 agcatatcaa actcaccttg tttcacccgc ttgcgtagtt cttcatcgta caacgaaccg
     1861 acaggagcat ctatgccttt ttctttgaga aaaacagaca tggctgctgc ttcggtgaag
     1921 gcttccggca ttttaggata cgccgtgacg acgatacgct ttgggatgaa aggcgaagac
     1981 atgccggaaa ttataacctt ttgtctttgc taacggatgt tatgccagcc gttgttcaca
     2041 aatatcatga tacccgcatc gcttacaacg ggacgcctgc tcatgcgacc gggccacatc
     2101 tctgcggacg tcgtcttcgc gcatatcggc aatgagttca atcagcgctg tttccagttc
     2161 acgagtgtag tcaatggcaa agtcgcggtt ctcgtagtgg atgatgccat agggtggacg
     2221 cctgccatag gtcttttcaa cgagcagaca ataggaagct aactgaaaaa tatgcgagtc
     2281 atacggcgct tcaggcgcac gccctgactt aacctccacg gggatgattt gtccgttctt
     2341 ttcgatcaaa taatcgggtt tgcctgtcag cccgagggct gcgtagaaga gaggcttttc
     2401 cactttgccc cagccgcgcg tgtcggtgta gatgacgcgt ccgccgggca agccggcggc
     2461 tttctgctgg cggttcgatt gccagaagaa gatgagggca agcaaaaaaa ggatgaggac
     2521 gatggatgtc atagcacttg aaaagcgaga tacgctacta aagtaagaat cgctgctaga
     2581 agcaggagcc agcccaaggt ccgcgtgaga gccgaggcaa ggacttggcg tccgtgtttg
     2641 cggtggagtt cggttccggc ggcaagctcc gctttgttct cggagggtgt cccgatcttg
     2701 cgataccacc aggcgcggcg acagtagagg taagaaccaa tttcagagga gcggatcggt
     2761 cgcatttggg tagtatagca caaattttct aattgaattg aggcgggaac ataatcgaat
     2821 cgtgataaac tttcggtgca aaatttcccc gtggagacgt cttatgacga aacatgatac
     2881 ttactcaatt gatattgctg gcgtaaaacg tgaattacgc ttgtttgaaa ttaaacctgg
     2941 gttgcgcatt gcgatcctga atattttggg tgacactgaa ttggtgcaag cctgcgcccg
     3001 cggacttgcc gataaagtga agaatgttga gtacgacatt atcgtgacgg ctgaagcaaa
     3061 gtccattccc attgcgcatg cgctttcggt ggaaaccaag aagccctatg tggtgttgcg
     3121 caagacctat aaaccgtata tgggcgatgc cctcaaagca gaaacgcttt cgatcaccac
     3181 gggtcaaccg caggttttga tcttggatga gaaggaccgt gagttgatcc aaggcaagaa
     3241 ggttttggta gtggatgatg tcatcagcac cggttcgacc ctgcagggca tgcgcatgat
     3301 ccttgataaa gccaatgcga ctgtggctgg tgaagcggca atcctcaccg agggcgaccg
     3361 ttcccagtgg atgaatatcc attcgctggg gcatttacct ttgtttactg attaaatgcg
     3421 taagggcgac tcgcgagtcg cccttacatc atgaacaatt ccattttctt ttttcttgag
     3481 agagccttga tggctctctc tcgttttaac gctgtgattt tatcgggctg ttcttcgagg
     3541 tagaccagtc tcacggggcg gcgggttcgg gtgtagcgtg cgccaacgcc tttgttgtgc
     3601 tgagacacgc gtcgttctgg gtcggtggtc cagccggtgt aaaaggttcc gtcggcacat
     3661 tcaaggatgt aacaaaagca gctcatccct tgcctttatc ttttcccaac ttgccaagtt
     3721 ttgagccaaa cagactttcc atgaaagatg gcttttcgat ctcttcccca ggaattctcg
     3781 accagtctgc tttttctcgt tcgacgagcc aactttccct gccttccttg gcagacttta
     3841 atcgctttac gaaagaagca tggtcttcat tttcaaggtc atcgcgcagg tcgagcagac
     3901 tgttgatcat cacattcaac gaatggatgg tatttgcgcg gttgtgttcc gcaagcatgg
     3961 caagcccttc caacgaatta tccaataggg cagaggtggc ggcgaaatat ccgcggctgg
     4021 cagatttttt cacttcgttc caccctggct gactcatcgt tccattcaat aaagcaacag
     4081 acgccaaacg tggcaggatg taagaagaag ccatcaaccc atcggattca acgacatcgg
     4141 tcagaatgga ggttgagccg aggagtttga cgaaatcggt cactagtttc acggcttcgc
     4201 cagatgtgcc agaaggggct gaaacgagga aaatgcccct ttcgaagagg tcagctttgg
     4261 ctgagtccag acccgaggca gtaacatcca aataagctgg tccaatcgcg ggcacaactc
     4321 caacgtaatg agtcgtttct ggcaagattt ccttgaccca tttcgtcact tcagccttga
     4381 ccggggcagt ttcgaccact acaacgtctt ggcgcaggtc ttgggcaatg tgcccaagcg
     4441 tttcgcgaat ttcattgaga ggtaaagcca aaatgaccag gtcggcattt tccaccgagc
     4501 tgggcaagtt atggttggtt tcattcacca cgccaagctt tttggcgcgc tgttctacac
     4561 tgtactcttt atcgtgaccg gtcgtcacca ctctgtcttt gtgtgcggca agcgccagcc
     4621 caatcgaagc cccaatttgc cccaaaccaa taattgtgat ctttacaggc atatcaacct
     4681 caaatctgga atggtcgcat tataaccccc gcttaaaata cgagaaccgc ggacgccgac
     4741 cgcggttctc tttgaaagga ggagaagaga aatcacctta cgcacgtaaa tttatcatgc
     4801 ttatccgaat actacttgac gcaaaaccta cgattaccct acgattgttt gacaacttgc
     4861 atgtctgtaa ttgcttttgg tattgtgtat ttgacaaagg cagaccctat gactttaact
     4921 ccttccaacc tttatcttgg tgaactaaat gacactcccc tcggagactt ccgcctcgtt
     4981 gcctcagacc tgggcttgat cgccgttgaa tgggcaaata accaatccga gctggatgcc
     5041 tacctacgaa agatcaaacg ctcctgtgag cccaaccaga ggaagattgc gccatatctc
     5101 aaagaactgc gtgagtacct taacggaaag cgcaccacct tcaccattcc catcgactgg
     5161 acgcccttta caccctttca acgcgaggcg ttacaggctg ttttccgggt tccctatggc
     5221 gaaacccgaa cctatattga catcgcccgc gaaattgacc atccgcatgc gtatcgggct
     5281 gtaggtgcgg cgaatgccat gaatcccatg ccgatcattg tcccatgtca tcgcattttg
     5341 gggacagatg gcaaactcca cggctatggt ggcggagatg gtctgccgac caaggagtgg
     5401 ttattaaaat ttgaaggcgt tacgatggca tgatacactc gagattgtgg acatacacct
     5461 tcaactcttt tggactttct taaaagttaa tttgttgagc acctcaggac ctgcttcggt
     5521 agggttgttg tatcacgaag ccgtagggcg ttttgtgaca gaggcgcagt ttgtacaggc
     5581 agtagggttt tcgtctgtgc tgccaggtag tgacgcatta caactggcaa tgtatattgg
     5641 gtatgcggca ggcggaatca ctggtggctt ggtcgctttg atcgcttcca ttttgccgcc
     5701 gactgtcatc atccttggtg tgacgatgat cttgcaccgt cttcggcgtg aggcatgggt
     5761 caatagcttt gtggaaggtc ttacacctgc cattgcggtt ttaatggtct tcaccgcatg
     5821 gaagatcttc gaaaaaggcg gcgggatgag ttggttcggt tggggaattg cactgaccag
     5881 tttccttgcc atgtggcgca aaatccctga acgcttcgtc atcatcattg ctgggatcat
     5941 cggcgttatc tttctttctg gctcatgaac acatcgaacc cttcaaaata ttcccaattg
     6001 ttctggatgt tcctgaaagt aaatttactt tctccttctg gtcccgcttc ggtggggttg
     6061 ttgtacaaag aagccgttgg cacgttaatg acagaagagc gctttgtgga agcggtgggc
     6121 ttttcgaact tcctgccggg gagcgaagcc ttaaagctgg cgatgttcgt aggttatgcc
     6181 gctgccggtg tgcccggggt ttttacggca ttgttgggtg ccatgcttcc gccgacagtg
     6241 atgatgttca ttgtggcgtt catcttgcaa caggtacagc atgaagattg
//
LOCUS       DAOQJE010000074         6282 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_6993_length_6282_cov_6.290027, whole genome shotgun sequence.
ACCESSION   DAOQJE010000074 DAOQJE010000000
VERSION     DAOQJE010000074.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 6282)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6282)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6282
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6993_length_6282_cov_6.290027"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..280
                     /locus_tag="PLT08_03130"
     CDS             <1..280
                     /locus_tag="PLT08_03130"
                     /inference="COORDINATES: protein motif:HMM:NF014025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MarC family protein"
                     /protein_id="HNE03486.1"
                     /translation="PIICGPGAITTSILLMNEADTMVEQGIVLGVIAVILLFTYLLLL
                     GAKQVTRIIGENGNKVMLRLMGLIVMVIAVEFFFAGLTPIVRKMLSGG"
     gene            complement(368..1855)
                     /locus_tag="PLT08_03135"
     CDS             complement(368..1855)
                     /locus_tag="PLT08_03135"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: protein motif:HMM:NF013592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family metallopeptidase"
                     /protein_id="HNE03487.1"
                     /translation="MKNKYLLFSAILLLALSCKALTPSTPTAIPQTETPPTEALPATE
                     PAVDEPIDPLAGSPGLGDSLYPDFGNGGYDATSYLLDITVNDVKTSDLAAITTIEAVA
                     TQDLSQFNLDFAGFEITSIMVNDEAAEFERDGQELTVIPAQALANGEAFSTVVEYQGV
                     PASMTSKALPFPTGWIAYENGIFVLSEPDGSASFYPVNDHPLDKAKYTIMVTVPKPYE
                     VGANGVLEETTDNGDTTTYTFTVRDEMASYLTTININEFNTETMVSDGGVPIRNYYAT
                     SLPDGVNKPFARQGEMIDYFSELFGPYPFEVYGALVMDTEFGAALENQTMSIFGIDMV
                     DLEDIEGTEAVVAHELVHQWFGDSVSVGDWSSIWLNEGFASYGEGLWEEHLNGRNGLN
                     EWVDGVYEEVKAYPEFYPPPGDPPANDLFNGGVYLRGGLTLHALRLEVGDDVFFEIIR
                     TYYDRYKYGNAVTDDFIAVAEEVSGKDLGEFFQAWLYDEKLPELP"
     assembly_gap    2031..2130
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2131..2681)
                     /locus_tag="PLT08_03140"
     CDS             complement(<2131..2681)
                     /locus_tag="PLT08_03140"
                     /inference="COORDINATES: protein motif:HMM:NF014069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HNE03488.1"
                     /translation="MTNEEIIQKTAEYIKQEFSDDSSGHDWWHIYRVWKNAIAICEIE
                     EADPFIVQLAALLHDLDDWKFNEAGDETPHRAKAWLASCSVSPYIADTVCKIIMHISY
                     KGAGVENKMDSLEGFIVQDADRLDAIGAIGIGRAFAYGGYKNRMMYDPDSPPAMHASF
                     EEYKNSKSATINHFYEKLLLLKDR"
     gene            complement(2683..3105)
                     /locus_tag="PLT08_03145"
     CDS             complement(2683..3105)
                     /locus_tag="PLT08_03145"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03667 family PPOX class F420-dependent
                     oxidoreductase"
                     /protein_id="HNE03489.1"
                     /translation="MIDFKSKLGRKALRHLKQEYFIWLTTVDSKGTPQPRPVWFVLEG
                     DSLVVYSQPSAFKLKHILNNPNVSLHFNTPDPKGEDDVIVFSGVAKIDPKGEPVNKNG
                     PYLRKYRSGIKGLNSTPQQFTQTYSTTIRIKLTSLRGW"
     gene            complement(3218..3577)
                     /locus_tag="PLT08_03150"
     CDS             complement(3218..3577)
                     /locus_tag="PLT08_03150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE03490.1"
                     /translation="MKWVIAMLVAVTAGYMLFDGVHALRTGDYITPSEGEYAGQLGPW
                     AGLVTSVGVDPHSTLMKTIFVAYGFSALVGVAGFLTDQVWGRSALLIMAVLGLWYLPI
                     GTAVNIVVLMLLFLKRN"
     gene            complement(3574..4065)
                     /locus_tag="PLT08_03155"
     CDS             complement(3574..4065)
                     /locus_tag="PLT08_03155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE03491.1"
                     /translation="MSTNHAHYLVEEYAFHNWLVHHILEGIDHEESLLQLPFESNSTN
                     WILGHIVTNRSHVLEVLGVTHLWQEEVRELYHTGTHNIGHGDKAMRLETLLKYMDDST
                     RMIRRALEPVSEEWLAESFTNYRGEKTREAHLSGFHWHEGFHIGQLEMLKDFVLSTRK
                     VLA"
     gene            complement(4084..4542)
                     /locus_tag="PLT08_03160"
     CDS             complement(4084..4542)
                     /locus_tag="PLT08_03160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HNE03492.1"
                     /translation="MSEDFRKNLLNFMTETFESPMGIYLDKNTALFQTLETVSAAEAS
                     IPVGGKCATLAAQVAHVTYYLDVLERYVVHNDTSPADWGEVWRTVSGVTTQEWETSKA
                     KLRSAYQRMVELFKTNPVWNETTMGGAMAIVVHSAYHLGEIRQALCTLKK"
     gene            complement(4542..5111)
                     /locus_tag="PLT08_03165"
     CDS             complement(4542..5111)
                     /locus_tag="PLT08_03165"
                     /inference="COORDINATES: protein motif:HMM:NF039496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5655 domain-containing protein"
                     /protein_id="HNE03493.1"
                     /translation="MSSLDKAVQTQLENIQKKTGKSLAELAAIAQKSGLTKHGELRDM
                     FKEKLGLGHGDANALVHAVFKSDGTRAAEGKSSDAVLDEIYVGAKASQRPIHEALMKQ
                     INKFGEFEVVPKKGYVSLRRKKQFAMIGPKTNTRFEVGINAKEMKKNPRLVEQPKGSM
                     CNYIVALTDASEVDAELIGWIKLAYEGAD"
     gene            5346..6047
                     /locus_tag="PLT08_03170"
     CDS             5346..6047
                     /locus_tag="PLT08_03170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE03494.1"
                     /translation="MNRLVLLAGLIITLVGCTTKSTPPPPTPIPATATQSVPTPTPVP
                     PTPTLVEIPVQVNAEVANCRFGPGTVYQTMNEVRGGRLLNAVGRDAASTWWQVEDPIN
                     PGGYCWVAAEVTTLQGTAEQLPIVDAPFVSVTKVDLRVEPNRIVVNCNDFPQTVFFEA
                     NITANGPTLLNWQWEASTGVTSDVGTLIFEESGTQVINEFYRINAPNEYWVKLKILSP
                     NQMEEQVTFPVSCNP"
     gene            complement(6092..>6282)
                     /locus_tag="PLT08_03175"
     CDS             complement(6092..>6282)
                     /locus_tag="PLT08_03175"
                     /inference="COORDINATES: protein motif:HMM:NF025380.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF4230 domain-containing protein"
                     /protein_id="HNE03495.1"
                     /translation="QEGLLVDIDPNLVLSTIQGGQEKILSVAIEDGILQQAERNAQAY
                     LIKFFNALGFNNVIFSEE"
BASE COUNT         1548 a         1552 c         1422 g         1660 t
ORIGIN      
        1 ccccatcatc tgcggaccgg gcgccatcac cacatccatt ctgctgatga acgaagccga
       61 caccatggtg gaacaaggta ttgtgcttgg ggtgattgct gttattttgc tttttaccta
      121 tttgctattg ctgggtgcaa agcaggtcac gcgcattatt ggggaaaatg gaaataaggt
      181 aatgctgcga ttaatgggtt tgatcgtgat ggtgattgct gtggaattct tctttgccgg
      241 gttgacacca atcgtgcgga agatgctttc gggtggctaa aggtttgaag cgctgaatca
      301 ggtttgagtt aagtttgagg cgttaaatgg atgaggtcac ctttacggtg acctcatcca
      361 tttaatccta tggaagttct ggaagttttt cgtcgtaaag ccaggcttgg aaaaattcac
      421 cgaggtcttt gccgcttacc tcttcagcaa cggcaataaa gtcatcagtg actgcgttgc
      481 catacttata acgatcatag tatgtgcgaa tgatctcgaa gaaaacatcg tctccaactt
      541 caagccgcaa ggcatggagg gttaacccac cgcgcaggta aacaccgcca ttgaagagat
      601 cgttggcagg ggggtctccg ggtggagggt aaaactctgg ataggctttt acttcttcat
      661 atacgccatc gacccattca ttaagcccat tacgtccgtt gaggtgttct tcccaaagac
      721 cttcgccata agaggcaaag ccttcgttga gccagatact gctccaatca ccgacactca
      781 cactgtcccc aaaccattgg tggacaagtt catgagcgac cactgcttct gtgccttcaa
      841 tgtcttccaa atccaccatg tcgattccaa atatggacat ggtttggttt tcaagcgcgg
      901 cgccaaactc agtatccata accaaggcgc catacacttc gaatggatag ggtccgaata
      961 gttcgctaaa gtagtcgatc atttcaccct gacgcgcaaa aggtttattg acaccatcgg
     1021 gcaaactagt agcgtaataa ttgcgaattg gaacacctcc atctgagacc atcgtttctg
     1081 tattgaactc attgatgttg atggtggtga gatagcttgc catctcatcg cggactgtga
     1141 aggtgtaggt ggttgtatca ccgttatctg tagtttcttc aagaacaccg ttcgccccga
     1201 cttcataggg ttttggcaca gtgaccataa tggtgtactt cgctttatcg agcggatggt
     1261 cattgaccgg gtaaaaactg gcggaaccat cgggttcgct caagacaaag atgccgttct
     1321 cgtatgcgat ccaaccagtg gggaaaggca gcgccttgga agtcattgat gcaggaacac
     1381 cttgatactc cacgacggtg gaaaaggctt cgccgttggc tagcgcttgc gctgggatga
     1441 cagttaactc ctgtccatct cgttcaaact ctgccgcttc atcattgacc atgatgctgg
     1501 taatctcgaa cccggcaaaa tccaggttga actggcttaa gtcttgcgtt gcaacagctt
     1561 cgatagtggt aatcgctgct agatcgctcg tcttcacatc gttgacggta atgtccaata
     1621 gataggatgt ggcatcataa ccaccattcc cgaaatcggg ataaagagaa tcgcccaaac
     1681 cgggagaccc tgccaatggg tctatgggtt cgtcgactgc gggttctgtt gcaggtaaag
     1741 cctctgtggg tggagtttct gtttgtggta tcgcagttgg cgtagaaggc gttaatgctt
     1801 tgcaggacaa tgccagcaat aagatcgcag agaacaaaag atatttattt ttcattggtt
     1861 tcctgaagtt gatttgatat ctgtcccttc ccattcaagc atgaactggt cgaggaattt
     1921 tgccatcaca tcatgccgtt cttcggcaat gcgcttggca gtgaacgtgt tcatacggtc
     1981 tttgagcagg agcagttttt cataaaagtg attgatcgta gcgcttttgc nnnnnnnnnn
     2041 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2101 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn cggtctttga gcaggagcag tttttcataa
     2161 aagtgattga tcgtagcgct tttgctgttc ttgtattctt caaagctagc atgcatggcg
     2221 ggtggagaat ctgggtcgta catcatgcga ttcttatagc cgccataagc gaaggcacgt
     2281 ccgatgccga tggctccaat tgcatcgaga cggtctgcat cttggacgat aaagccttcg
     2341 agcgagtcca ttttgttttc tacaccggca cctttatagg aaatgtgcat aatgatcttg
     2401 cacacggtgt cagcgatgta tgggctcaca gagcaagagg caagccaggc tttggcgcga
     2461 tgcggcgttt cgtcgcccgc ttcgttgaac ttccaatcat cgaggtcgtg taagagagca
     2521 gcaagctgaa caatgaatgg atcagcttct tcgatctcac agatggcaat ggcattcttc
     2581 cacacacgat aaatgtgcca ccaatcatgc ccagaagagt catctgaaaa ctcttgcttg
     2641 atatattccg cggtcttttg aataatctct tcgttcgtca tatcaccaac cacgcaacga
     2701 tgtgagtttg atgcgaatcg ttgtggagta tgtttgcgta aattgctgcg gcgtggagtt
     2761 taatccctta atgccgctgc gatacttgcg caggtagggt ccgttcttgt taactggctc
     2821 acctttgggg tcgatcttgg caaccccgct gaacacaatc acatcatctt cgcctttagg
     2881 gtctggggtg ttgaaatgca aagagacatt ggggttattc agaatgtgct tgagcttgaa
     2941 ggcactgggc tggctgtaaa caacaagaga atcgccttcc aacacaaacc aaaccggtcg
     3001 cggctgaggt gtgcctttcg agtcgacggt ggtgagccag ataaagtatt cctgcttgag
     3061 gtgacgtaag gctttgcgcc ccagtttgga tttgaaatcg atcatttctt cctccattca
     3121 taaattcgat aatgcctgca cttaaaatca ttataaacga acaggctggg catgttgccc
     3181 agcctgctct tacggatgaa atattttttc atgatattca atttcgtttc aggaacaaga
     3241 gcatcaacac cacaatgttg accgcagtac caatagggag ataccacaac ccaagcactg
     3301 ccataataag gagtgcacta cgcccccaaa cttggtctgt gagaaaacca gcaaccccaa
     3361 ccagcgcaga aaaaccataa gcaacaaaaa tggttttcat caatgtggag tgtgggtcta
     3421 cacccacaga tgtgacgagc ccagcccaag gtccgagttg acccgcgtat tcgccttcag
     3481 atggcgtgat gtaatcgccc gtccgcaagg catggacgcc gtcgaagagc atgtatcctg
     3541 ccgtgacggc aaccagcatg gcaatcaccc acttcatgcc aacacctttc gggtagaaag
     3601 gacaaaatct ttcagcattt ccaactgacc aatatggaac ccttcgtgcc agtggaatcc
     3661 gctcaggtgc gcttcacggg tcttttcgcc gcgatagttg gtaaagcttt ctgccagcca
     3721 ctcttcgctg acaggttcca gcgccctgcg aatcatgcgc gtactatcat ccatgtactt
     3781 caaaagagtt tccaagcgca tggctttatc gccatgacca atattatgag tgccggtgtg
     3841 atacagctcg cggacttctt cctgccagag gtgtgtgact cccaatactt ccagtacatg
     3901 gctgcgattg gtaacgatat gaccgagaat ccaatttgtt gagttggatt caaagggcag
     3961 ttgtaacaaa ctttcttcgt gatcgatccc ttcaagaatg tgatgaacca accagttatg
     4021 aaaggcatat tcttcaacta ggtaatgggc atgattggtc gacatgtgta tgtttcctct
     4081 tttttatttt ttcaacgtgc aaagcgcctg acgtatctct cctaggtgat aggcactgtg
     4141 aacaacaatt gccatggctc cgcccatcgt ggtttcgttc caaactgggt ttgtcttgaa
     4201 taactccacc atgcgttgat aggcacttcg caatttggct ttactcgttt cccattcttg
     4261 cgtcgtgacg ccgctcaccg tgcgccaaac ctcgccccaa tcggcaggcg atgtatcgtt
     4321 gtgaactaca taacgctcca gcacatcaag ataataggtc acatgcgcaa cctgtgccgc
     4381 gagcgtggcg cacttcccgc caacgggaat cgaagcctcc gctgcagaga ccgtttcgag
     4441 cgtctgaaac agagccgtgt tcttatcaag gtaaattccc atggggcttt caaaggtctc
     4501 ggtcataaag ttcaaaagat tttttctaaa atcttccgac attaatcagc tccttcatat
     4561 gcaagtttga tccagccaat cagttcagca tccacttcgc tggcatccgt cagggcaacg
     4621 atgtaattgc acatactgcc tttgggttgt tctaccaaac gcggattttt cttcatctct
     4681 ttggcattaa tacccacttc aaaacgagta ttggttttag gaccgatcat cgcaaattgc
     4741 ttcttgcgcc gcaaacttac atatcccttc ttcggcacaa cctcaaactc tccgaacttg
     4801 ttgatctgtt tcatcaacgc ctcatggatg gggcgctgcg aggctttggc accgacgtaa
     4861 atttcatcta aaacagcatc agagcttttc ccttcggcag cgcgtgtacc atcagacttg
     4921 aaaaccgcat gcaccagtgc atttgcgtcg ccatgcccaa gccccagttt ctccttgaac
     4981 atatcgcgca gttcgccatg cttggtcagc ccactctttt gagcaatggc tgccaactct
     5041 gccagcgatt taccggtctt cttttggatg ttttccagtt gtgtttgaac ggctttatca
     5101 agactactca tactctcatc tccttttgaa taaaacaacc gagttacaca atggtacaat
     5161 tatagaacaa attttctaat tttgcaattg cttatcccca caaagtacca atttaattct
     5221 atgagcgcat ctgaacttca gcttccctgt tacacttcta tagaacatgc aggtgattcg
     5281 accccgaatt gccttgcctc tccaacgccg atggagagtt gaaacgaatg acaaaaggag
     5341 gaaaaatgaa ccgcctcgtc ctgcttgcag gactcattat caccttggtg ggatgcacaa
     5401 cgaaaagtac gccgcctccc cccacgccga ttccggctac tgccactcaa tctgttccta
     5461 cccccacacc tgttccaccc acccccaccc ttgtagagat tcctgtacag gtgaacgcag
     5521 aagttgccaa ctgccgtttt ggtcctggca cggtctacca aaccatgaat gaagtccgcg
     5581 gtgggagact cttaaacgct gtcgggcgcg acgcggcatc tacctggtgg caggtcgaag
     5641 atcccatcaa cccaggcgga tattgctggg ttgcagccga agtcaccacc ttgcaaggaa
     5701 ccgcggagca actacctatt gtagatgccc ccttcgtcag cgtcaccaaa gtggatttgc
     5761 gcgtggagcc gaatcgtatc gtcgtcaact gcaacgactt tccgcagacc gtcttctttg
     5821 aagccaacat caccgccaac ggaccaaccc tccttaattg gcaatgggaa gccagcacag
     5881 gcgtcacctc agatgtaggc acattgatct ttgaagagtc tggcacacaa gtcatcaatg
     5941 agttttaccg catcaacgcg cctaatgagt attgggtaaa actaaaaatc ctttcaccca
     6001 atcaaatgga agagcaagtg accttccccg ttagctgtaa tccttaaata aaaaaggcga
     6061 ctcacatgag tcgccttttt tattgttttc tttattcttc agagaaaatt acattattaa
     6121 agcccaacgc attaaagaat ttgatcaaat acgcctgagc atttcgttct gcttgctgca
     6181 aaatcccatc ttcgatggca acgcttagaa ttttctcctg cccaccttga attgtggata
     6241 gcacaaggtt tgggtcaata tctactaata aaccttcttg ca
//
LOCUS       DAOQJE010000075         6258 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_7036_length_6258_cov_6.766242, whole genome shotgun sequence.
ACCESSION   DAOQJE010000075 DAOQJE010000000
VERSION     DAOQJE010000075.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 6258)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6258)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6258
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7036_length_6258_cov_6.766242"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            47..670
                     /gene="udk"
                     /locus_tag="PLT08_03180"
     CDS             47..670
                     /gene="udk"
                     /locus_tag="PLT08_03180"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004849 - uridine kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HNE03496.1"
                     /translation="MMHKIPLIIGVAGGSGSGKTTVAQELLRRVGADRIAYLQHDSYY
                     KDLRMLPSTQHSEINFDHPDTLETELLIKHIIDLREFKAVQIPIYDFSTDTRKTETIT
                     VEPRNVILVEGILLYVEPELRKLFDVKLFVDADADIRFIRRLHRDITERGRSTESVIE
                     QYYATVRPMHLEFVEPSKRYADVIIPEGGHNVAALDMVTARIELLLK"
     gene            794..1216
                     /locus_tag="PLT08_03185"
     CDS             794..1216
                     /locus_tag="PLT08_03185"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF012387.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0016018 - cyclosporin A binding [Evidence
                     IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HNE03497.1"
                     /translation="MVIELFADKAPVTVNNFVFLSREGYYDGVIFHRVISNFMAQGGD
                     PTGTGRGGPGYKFQDEFHPSLKHSKRGILSMANAGPGTNGSQFFITHGPTPHLDNRHT
                     VFGEVVEGEDVLMSIPERDPMKLNAPAVKIIRVTIEEI"
     gene            1194..1766
                     /locus_tag="PLT08_03190"
     CDS             1194..1766
                     /locus_tag="PLT08_03190"
                     /inference="COORDINATES: protein motif:HMM:NF020893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HNE03498.1"
                     /translation="MSPSKKSKPENKVVTAILRILAVLAVIGITVYVYSIRNHVEEFA
                     NYGYFGAFLIMLIANATVILPAPGVAIVFAMGSMLNPLGVALAAGVGGMLGEMTGYLA
                     GYSGQAAVENTTVYNRIVPYVQKYGAWVILVMSAIPNPFFDLAGIAAGIAKIPVWQFM
                     LACWVGQTIKMSMFAFAGAYSIDWIARLYK"
     gene            1824..>2383
                     /locus_tag="PLT08_03195"
     CDS             1824..>2383
                     /locus_tag="PLT08_03195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HNE03499.1"
                     /translation="MSNYDKVDAFIDKNLSQSLDELKKYVAQPSISAQNLGLKECAQL
                     VKEMLEKRGFTAEIMATEGAPVVFAERKGNVDKTILIYNHYDVQPPEPLELWDSPPFE
                     PEIRDGKMYGRGVSDDKGHLTSRLHAIDAILAEDGELPCNIKFIIEGEEETASVHLHE
                     FISNNVERLKADACIWEFGSVDHRDRP"
     assembly_gap    2384..2483
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2484..2996
                     /locus_tag="PLT08_03200"
     CDS             <2484..2996
                     /locus_tag="PLT08_03200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HNE03500.1"
                     /translation="LEGGMDLKLEEVFTPTCTICGLTSGYQGPGSKTVQPASASAKVD
                     FRLVPNQMPKDILKKLRAHLDAEGFSDVEIKFLGGEPAARTDPDHPFVKLVSETAEEV
                     YDAKMDIVPMIGGSGPNYPFVHELGLPVVCMGIGYPNTRAHAPNENFRIDLYAQHAKH
                     TARVIREFAK"
     gene            3172..3813
                     /locus_tag="PLT08_03205"
     CDS             3172..3813
                     /locus_tag="PLT08_03205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS and ACT domain-containing protein"
                     /protein_id="HNE03501.1"
                     /translation="MFVGERMSHPVITVTPETPVHDALAMFKKEHIRRAPVVKDGKLV
                     GIVSESDLLNASPSPVTSLSVWEMNYLISKVTVKQVMSKKLITVDVNTPIEEAARIMA
                     DKKLGGLPVMRSDKLVGIITETDLFKIFLELMGARTKALRVTAEIDDKPGTLAKLTKA
                     VSDAGGNFISFGQFSGDDTSSKILTFKVAGLKKDEVTSALSKVVKKFWDIRQS"
     gene            complement(3882..5570)
                     /locus_tag="PLT08_03210"
     CDS             complement(3882..5570)
                     /locus_tag="PLT08_03210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE03502.1"
                     /translation="MKKYPFFGLISILVLSSLILSACGVSEDYEEDFSTEEVATEEIV
                     TEEATQASSDATSITPSAPGDVIYDFGFTPEENGFSFENYGDEIPATNLTADELRRMF
                     GDEVCADINGDECILTPPAAQWMEELNTDMSGGHCEGMAVLSLVMYTGQVSPSEFGGN
                     TASELDITDKALQREIAYWWATQAVDPTASSIISGTPMEILDLVEKMDVKGETYTIGI
                     YNENGDGHAITPFGVEDKGDGLYAILVYDNNYPGETRELFIDSRDNSWTYETAINPAV
                     ETDVWSGNADTQTLDLTPTSARFEPQVCPFCEGGYSSVGKLAAATQPLNQIFLDGEGH
                     ILITDDNGNRLGYVDGKIVNEIPGASYTKYRMLANGETPEPIYAVPADLDITVTIDGS
                     ALTEETLTDLVLIGPGYSIGVEGIYLTPGQVDVAYFYPTDEMIAYETTSDESPSIIFG
                     VENPDADYYFEVYSADMIGGGTITASLDSKAGDLLINTEQLTGEGSFNFYLTRITDEA
                     EEEFSAEDITLQEGALIYVNYAEWTDANPEGIYFGVDLNGDGEIDDEYVVDDAN"
     gene            complement(5642..>6258)
                     /locus_tag="PLT08_03215"
     CDS             complement(5642..>6258)
                     /locus_tag="PLT08_03215"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNE03503.1"
                     /translation="QLYHHVARHADWTNADALEVSSGRGGGASFIMRHFKPRSYVGVD
                     FSAKAIEFCREHHKVDGLRFEHGNAEDLQFPDNSFDIVVNVEASLYYPNIKKFFQHVK
                     RVLKPGGYFLYTDLRYEEKVEEWHAQIKEMGFKVIKEEDITENVLKAMEMDRERRIHL
                     VKTYIPRILQKQFFHFAGLDVESPTSAQPHLEQRRYWYFALQKM"
BASE COUNT         1655 a         1447 c         1429 g         1627 t
ORIGIN      
        1 ggagccgttt gcgtcccaat gagagtggcg ggtataatct cgtcatatga tgcataaaat
       61 ccctttgatt attggagttg cgggcgggtc aggttcgggc aaaacaacgg ttgcacagga
      121 attattacga agagtggggg cagatcgaat tgcttatctt caacacgatt catattacaa
      181 agatttgcga atgttgccat ctacgcaaca ttctgaaatc aattttgatc atccagacac
      241 gcttgagact gagttattaa tcaaacatat tattgacttg cgcgagttca aagccgtgca
      301 gattccgatt tatgattttt caacggacac acgtaagaca gagacaatca ctgtagaacc
      361 ccgaaatgtg attttagtgg aagggattct tttgtatgtg gagcctgagc tacggaagct
      421 ttttgatgta aaactttttg tagatgcgga tgcagacatt cgcttcattc gccgcctgca
      481 cagagacatc accgaacgcg gacgatccac tgaatctgtg atcgaacaat attatgcaac
      541 tgtgcgccca atgcatttgg aatttgtaga accttcaaag cgctatgcag atgtcattat
      601 cccagagggc gggcataacg ttgccgctct ggatatggtt acagcaagaa tcgaattgct
      661 gctcaaataa acccaatttt atattcatct actcaaggag agacgtatgg caaaacaatg
      721 gagcaccccc ccggcaatgc agatcgaccc caagaaaaag tacaaagctc acatggaaac
      781 agataacggt acgatggtga ttgaactgtt cgctgataag gcgcctgtga ccgtgaataa
      841 ctttgtgttt ctttcgcgcg aaggctatta tgacggtgtg atctttcatc gtgtgatctc
      901 aaactttatg gcacaaggcg gcgacccgac cggtaccgga cgcggcggac ccggctataa
      961 atttcaggat gaattccacc caagcctgaa gcatagcaag cgcggcatcc tttccatggc
     1021 aaatgcagga cccggcacaa acggctcgca attcttcatt actcacggac ccaccccgca
     1081 cttggataac cgtcacaccg tgttcggtga agtggtggaa ggcgaagatg tgttgatgtc
     1141 cattcctgaa cgcgacccga tgaagctcaa cgccccggct gtaaagatca tccgtgtcac
     1201 catcgaagaa atctaagcct gaaaataaag tggtcaccgc catactgcgc attctggcag
     1261 tactggcggt gattggcatt acggtctatg tgtacagtat ccgtaatcac gtagaggaat
     1321 ttgccaatta tggttatttt ggcgcgttcc tcatcatgtt gattgcaaat gcaaccgtga
     1381 ttctaccggc tccgggtgtg gcgattgttt tcgccatggg cagtatgctg aatccacttg
     1441 gggttgcact ggctgcaggg gtcggcggta tgttaggtga aatgacaggc tatttggcag
     1501 gctatagcgg gcaagccgct gtagaaaata caacggtcta caaccgcatt gtgccatatg
     1561 tgcaaaagta tggtgcctgg gtcattctcg ttatgtcggc aattccaaat ccattttttg
     1621 acttggctgg cattgcagca ggcattgcga aaattcccgt ctggcagttt atgttggcat
     1681 gctgggttgg gcaaacaatc aaaatgtcta tgtttgcttt tgccggagcc tattccattg
     1741 actggatcgc ccgtttatat aaataaccaa aacctgacag gtgctctgtc aggtttattt
     1801 ttatctcagc aaaggagttt cagatgagca attatgacaa agtcgatgcc ttcatcgata
     1861 aaaatctaag ccagagttta gatgaactca aaaagtatgt tgcccaacca agtatcagcg
     1921 ctcaaaactt gggactgaag gaatgtgccc aacttgtaaa agaaatgctg gaaaagcggg
     1981 ggttcacagc tgagatcatg gcaacagagg gagcgcctgt tgtctttgca gaacgcaaag
     2041 gcaacgtgga taaaaccatc cttatctaca atcattacga cgttcagccg ccagagccat
     2101 tggaattatg ggatagcccg ccttttgagc cagagattcg ggatggcaag atgtatgggc
     2161 gcggtgtgag cgatgataaa ggtcatctca catcacgctt gcatgcgatc gatgcgatcc
     2221 ttgcagaaga tggcgaatta ccttgcaata tcaagttcat cattgaaggt gaagaagaaa
     2281 ctgccagtgt gcacttgcac gagtttatct ccaataatgt agaacgactc aaagccgatg
     2341 cctgcatttg ggaatttggc agtgttgatc atcgcgaccg cccnnnnnnn nnnnnnnnnn
     2401 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2461 nnnnnnnnnn nnnnnnnnnn nnnttggaag gcggcatgga tttgaaactt gaagaagttt
     2521 tcacgcctac ttgcaccatt tgcggtctca ccagcggata ccaaggtccc ggctcgaaaa
     2581 ccgttcagcc tgcctctgcc tctgcaaaag tggacttccg ccttgtgccg aatcaaatgc
     2641 cgaaagacat cctcaaaaaa ctgcgcgccc atttggatgc cgaaggcttc agcgacgtcg
     2701 aaattaaatt cttgggtggc gagcctgcgg cgcggacaga tccggatcat cctttcgtca
     2761 aactggtcag tgaaactgca gaagaagtct acgatgcgaa gatggacatt gtccccatga
     2821 tcggcggttc tggacctaat tatccgttcg tgcatgaact tggattgccc gttgtgtgca
     2881 tgggaattgg gtacccgaat acaagggcgc acgcccctaa cgagaacttc cgtattgatc
     2941 tttatgcgca acatgccaag catacggcaa gggtcattcg agagtttgcc aagtaagtac
     3001 aagacaaaag accacgagca atcattgttc gtggtctttt gtatcggtcg ccaatgacat
     3061 gcctaaagtg acctttttca ctccaattta gtgactttct ttttgcaaat tctgcgtata
     3121 atgtgattgt aataaatttc acaatataac ttcaccccat tggaggaaac aatgttcgtc
     3181 ggcgaaagaa tgtctcaccc tgtaattacc gtcactccgg aaaccccggt tcatgatgca
     3241 cttgctatgt tcaagaaaga acacatccgc cgggcacctg ttgtcaaaga cggaaaactt
     3301 gtcggcattg tttctgaaag tgaccttttg aacgcctcac cttctcccgt caccagcttg
     3361 agcgtgtggg aaatgaacta ccttattagt aaagtcaccg tcaaacaggt tatgagcaag
     3421 aagttgatca ccgtcgatgt caacaccccc atcgaagaag ctgcccgcat tatggcagat
     3481 aaaaaattag gtggtttgcc tgttatgcgc tccgataaac tcgtaggcat cattactgag
     3541 accgatctct tcaagatttt tcttgaactg atgggcgccc gcaccaaagc tttgcgtgtg
     3601 actgccgaaa ttgacgacaa acccggtact cttgccaaac tcaccaaggc agtatcagat
     3661 gcgggcggca atttcatctc ctttggtcaa ttctctgggg acgatacttc cagcaagatc
     3721 ctcactttca aggtagctgg tttgaagaaa gatgaagtta ccagtgcatt gagcaaggtt
     3781 gtaaaaaaat tctgggatat tcgccagagc taaattaaca aagaaaatag cgcatggact
     3841 tctcctctcc atgcgctatt tttatttaag tgttcaatag cttagttggc gtcatctact
     3901 acatattcgt catcgatctc gccgtctcca ttgaggtcca cgccaaagta gataccttca
     3961 ggattggcat cggtccattc ggcgtagttg acgtagatca atgcgccttc ttgcaaagta
     4021 atgtcttcgg cgctgaattc ttcttcggct tcatctgtga tgcgggtaag atagaagttg
     4081 aaagagcctt cgccagtcaa ttgttccgta ttgatgagca agtctccagc cttgctatca
     4141 agtgaggcgg taatggttcc gcccccgatc atatcggcgc tatacacttc gaagtagtaa
     4201 tcggcatctg ggttttctac accgaaaata atgctggggg attcatcgga agtggtttcg
     4261 taagcaatca tttcatccgt tgggtagaaa tacgccacat ccacctgacc cggtgtgaga
     4321 taaatgcctt ccactccaat agaatagccg ggtccgatca aaacgaggtc ggtgagggtt
     4381 tcttcggtca aggcggagcc gtcaatggta acggtgatat ctaaatcagc agggacggca
     4441 taaataggct cgggggtttc tccattggca agcatacgat atttggtata ggaggcgcca
     4501 ggaatttcat tcacaatctt gccgtctaca tagccaaggc gattgccatt gtcatcagta
     4561 atcagtatgt gaccttcgcc atccaagaag atctggttaa gaggctgggt tgctgcggca
     4621 agtttgccta cactagagta tccgccttca cagaaggggc aaacttgggg ttcgaagcga
     4681 gctgaagtcg gagtcagatc gagtgtttga gtatctgcat ttccagacca gacgtccgtc
     4741 tccactgctg ggttgattgc agtttcgtag gtccaagaat tatcacgtga gtcgatgaat
     4801 aactcgcggg tctcgccagg gtagttattg tcgtaaacca gaatagcata caaaccatcc
     4861 cctttgtctt cgaccccaaa cggagtaatg gcatgaccat cgccgttttc attataaata
     4921 ccaatggtat aggtttcgcc cttcacatcc attttttcaa ctagatccaa aatctccatg
     4981 ggtgtgccgc tgatgatgct ggacgctgtt gggtctacgg cttgggttgc ccaccagtac
     5041 gcaatttcac gttgcagtgc tttatcagtg atatctaatt cactagccgt atttccacca
     5101 aactcagagg gagaaacctg accggtatac atcaccaagc ttaacacagc catgccttcg
     5161 caatgaccac cggacatatc ggtgttgagc tcctccatcc actgtgctgc tggcggggtg
     5221 agaatacatt catccccatt aatatctgca caaacttcat caccaaacat acggcgtaac
     5281 tcatctgccg tcaaattagt agcagggatc tcatcaccat aattttcaaa actaaacccg
     5341 ttttcttctg gggtaaagcc aaaatcgtaa atcacatcgc caggggcgct gggtgttata
     5401 gaagtcgcat cactacttgc ttgtgtcgct tcttctgtga caatttcttc tgtagcaact
     5461 tcttcagtgg agaagtcttc ttcatagtct tctgaaacgc cacaagctga aagaatcaaa
     5521 ctactcaaaa ccaaaatcga gatcaatcca aaaaacgggt actttttcat atttcctcta
     5581 gtgaatttat tacatcaaac ctgcgtaaca atcgtcacgc aggttatctt tcgtattagt
     5641 tttacatctt ttgtaaagca aaataccaat aacggcgttg ctctaaatga ggctgtgctg
     5701 aagttggaga ctctacatcc aacccggcaa aatggaagaa ctgtttttgc aaaatgcgcg
     5761 gaatataggt cttcacaaga tgaatacggc gctcgcgatc catttccatt gctttgagaa
     5821 cattctctgt aatatcttct tccttgatca ctttgaagcc catctcttta atttgtgcat
     5881 gccattcttc cactttttct tcgtagcgca ggtcggtgta caagaaatat ccacccggct
     5941 tcaagacacg tttcacatgc tggaaaaact tcttgatatt cgggtaatac aaagatgcct
     6001 ctacattgac cacaatatca aaagagttat cgggaaattg caaatcttct gcgttgccat
     6061 gctcaaatcg aagtccatcc actttatgat gttcacggca gaactcgata gccttggcag
     6121 aaaaatccac gccgacgtaa gaacgtggtt tgaaatgacg catgataaaa gacgcgcctc
     6181 ctccccgccc cgagctgacc tctaacgcgt cagcattggt ccaatctgca tgccgggcga
     6241 cgtgatgata caactgca
//
LOCUS       DAOQJE010000076         6231 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_7092_length_6231_cov_7.062176, whole genome shotgun sequence.
ACCESSION   DAOQJE010000076 DAOQJE010000000
VERSION     DAOQJE010000076.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 6231)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6231)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6231
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7092_length_6231_cov_7.062176"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(277..1836)
                     /locus_tag="PLT08_03220"
     CDS             complement(277..1836)
                     /locus_tag="PLT08_03220"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HNE03504.1"
                     /translation="MANNLIGKTISNRYVIEEMLGQGGMSAVYKGNDPNLKRVVAIKV
                     IHSHLSGNPDFVQRFEEEAAAVAQLRHPGIIQVYDFNRDDDLYYMILEFVPGETIQDH
                     LKRLNDSGRKLSPTKAAEYMAGICDAVDYAHQRGMIHRDIKPANLMLTITGQVILMDF
                     GIAKIVGGTRHTATGAVVGTAMYMSPEQIKGEQPDRRTDIYSLGVTLFEMVSGRPPFE
                     AESAMTLMMMHINDPVPDPKKLNPDVPDALVAVIQKALAKDPNNRYQSAAQMAAALRN
                     LLSAGSSSPLATMMEDATIMNSSRGTMIEKPYAGTYVESKPIAPAPGTMNKNSAPSSM
                     YATPAPAASAPKRKNLLLLPIIAVVAIGLICLVGGVAFAAKQLFGGVVETEPPVVLAT
                     NTQPAIVAPIETEVSTETPIPATPTPSSPYVVITAIRVEGSLYAVDYDVHNFTDGLHV
                     HIFFNTVPPEQAGSPGSGPWKLTWGAYGNPPFTQYSIAARPANATQMCALVANSNHSV
                     QPNSGNCVDLP"
     gene            complement(1926..2843)
                     /locus_tag="PLT08_03225"
     CDS             complement(1926..2843)
                     /locus_tag="PLT08_03225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE03505.1"
                     /translation="MLISLACGTSGSTASRESTVEAISAQVRATGTAAAAQSSQGTQP
                     VVETNSDSLTATAVAFAPILESLPKYGVDPSEGKIGWIHPPVSLDASGYHQFEYINYY
                     IGTLAKDFVISSDITWNTVGSISGCGFVMRSDGNTNALDQYVAVITRVASGHFVFSTM
                     SDGEVVTGRDIYARYKDKSFRWENDTTNRLTIVGRGNHFWIYTNDTLIGEVDPSAPPP
                     QPNLPPEPDKPANPSDPQAMALYEQQKIEREAIIAQMQADYAARKRAYNSAETVFERG
                     FMALVALSESGRSTNCKFDNTWLFLIDSP"
     gene            complement(2889..6086)
                     /locus_tag="PLT08_03230"
     CDS             complement(2889..6086)
                     /locus_tag="PLT08_03230"
                     /inference="COORDINATES: protein motif:HMM:NF013245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HNE03506.1"
                     /translation="MNSFKLSLINDQSSTPSKEFELTKSEIIIGREDSVDITIPASAV
                     SRRHARLMLIGENYVLEDLGSSNGTYVNGEKIDKQHTLKSGDKIRLGQIISLVYTAPV
                     PEEDSNLTVVSIKDANKTAKHSGPAKPAHVMQTVLGEAPAIPQASAGPRQLIVSIAGG
                     STQTYPLTGSSISIGRLDTNDIVIPSQIVSSRHARLEQVKGGGYKLVVMPEAKNPVQF
                     EGRPIDGSHVLHHNDLLRIGSMDPGVMVTITYNAPSEASQQMERDIVFGEKTLIQIGR
                     DPSNDVVIPSPNVSRFHTQIEKVGQRYRVEDLRSSNGTYVNGERIEGSVWLKPNDSIR
                     IGQYRFVMGKDQLARYDDGNGLRVDVVGLNKWVRKDLNILQNIYASFSPREFIVVVGQ
                     SGGGKSTFVDAVAGYRPATPPSKVYVNDIDIYTHFDAIRNEIGFVPQKDIIHMELTIY
                     QALDYAAQLRMPADTSPEERHKRIMEVLEDLDLTHRKDVQISGLSGGQQKRVSIGVEL
                     LTKPGLFFLDEPTSGLDPGTETALMQLMRRLADQGRTIILITHATKNVMLADKVIFLA
                     RGGYLAWFGPPDEALAYFDQYRSERARRAGKIEFDEIYAILDDPSKGKAEDWANRYRS
                     SNAHTEYVLKPLAGKLSPQSVATVPQPERLPQKAANTRQVSSLRQLMILSARNIKILT
                     RDRFALGLMLATAPVVSLLDVILSFVMGREIFDFYKGDMSQVMITLFLLTIYGIMVGG
                     ISQMREIVKEQDIYRRERLVNLKIFPYVLSKVWVAALLALYQAVAYTVVHYISFRMPG
                     GVMEFIQIYITMTLATFAGMMLGLFASALAPNSSSAPLLVIILMLPQIVLAGALIPVP
                     SFVSAPFSTRWAFESLIAISGSGSDVAADVCWALPPDVRNAMSLEEKLANNCKCMGVN
                     LLKKESCNHPGIGKFYIPLVDQPLPIPPAPLPPEPVRPVEPQDQSDNIAMADFFAKLK
                     IYEAEVELYKAKVDKYQEEQVAYQTVISQLKVQVATAEAVVRTFVNGSGFAYVDKDDP
                     LAYHTKVILTWTVQSFIIVLLFVAILYLQKRKDVI"
BASE COUNT         1607 a         1426 c         1442 g         1756 t
ORIGIN      
        1 aatacggcat cctcatcaaa gaacgccgct acttgcgccg ctctgtcttc atcgtcgggc
       61 gagatggaaa attgacctat gtcaattacc tgcctgcact gggtgatgaa ccaaattatg
      121 aagaggtcat cgcagcggca aaggatgcat tgaaggcata aagaacaaac acccttttgc
      181 aagggtgttt gttcttttat atgcaatagt ttgtttatgc cgttacagcg ggtctaaatt
      241 tatacacgta tacgtgatat ataccgtgag ttacttttac ggcaaatcta cgcagtttcc
      301 agagtttggc tgaacagagt gattcgaatt ggcaactaat gcacacattt gagttgcgtt
      361 cgccggtcga gcggcaatgc tgtattgagt gaagggtggg ttgccatatg ccccccaggt
      421 caacttccag ggacctgatc caggcgagcc tgcttgttct ggcggaacag tgttgaaaaa
      481 tatatgcacg tgcaatccat cagtgaagtt gtgtacatca taatccacag cgtaaagact
      541 tccttcgact ctgattgcgg tgatgactac gtaggggctc gaaggggttg gtgttgccgg
      601 gattggagtt tcagttgaga cctctgtttc aataggagct acgattgcgg gttgagtatt
      661 ggtcgcgagt acaaccggcg gctctgtttc tacaacacct ccaaagagct gcttggctgc
      721 aaaagcaact cctccaacaa gacaaattaa tccaatagcc actacggcaa tgatcggcag
      781 aagtaacaaa ttttttcgct ttggagcaga agccgccggc gccggggttg cgtacatcga
      841 acttggcgct gaatttttgt tcattgttcc aggtgcagga gcgataggtt tactttccac
      901 atatgtgcct gcatatggtt tttctatcat tgttccacgc gatgaattca taatggtggc
      961 atcttccatc atcgtggcga ggggtgaaga tgaaccagcg gaaagcaaat ttcttagtgc
     1021 ggctgccatt tgagcagcgc tctgataacg attgttcgga tccttcgcaa gcgctttttg
     1081 gatgactgcg accaaagcgt ctggcacatc aggattgagc tttttaggat ccggcaccgg
     1141 atcgtttata tgcatcatca tcaatgtcat ggctgattcg gcttcaaaag gcggacgtcc
     1201 gctgaccatt tcaaacaagg ttacgccgag agagtaaata tctgtgcgcc ggtcgggttg
     1261 ttctcctttg atctgttcag gcgacatata cattgcggtt ccgacaaccg ccccggttgc
     1321 tgtgtgacgt gtaccgccaa cgatcttggc tatgccgaaa tccatcagaa taacttgacc
     1381 tgttatggta agcattaagt tagctggttt aatatcgcgg tggatcattc cacgttggtg
     1441 cgcataatcg actgcatcgc aaatgcctgc catgtattca gcggctttgg tgggggagag
     1501 ttttctaccg ctatcgttga gtcttttcaa gtgatcctga attgtttctc cgggcacaaa
     1561 ttccaaaatc atataataaa ggtcatcatc gcgattgaag tcatagactt gaataatacc
     1621 gggatggcgt aattgcgcta ctgctgcagc ttcctcttca aaacgctgga cgaaatcagg
     1681 gttgcccgat aaatgggagt gaatgacctt gatagccaca acacgcttta agttggggtc
     1741 attgcctttg taaactgcag acatcccacc ttgccccaac atttcttcga taacatatcg
     1801 attgctaatg gtctttccaa ttaagttatt agccattgct actcctagaa aataaaaaca
     1861 ttgacactct taaataatat tcgtaatact gaaataacgc tcagctttat accctacttt
     1921 aatccttatg gtgaatctat gagaaaaagc catgtgttat cgaacttaca attggtactt
     1981 cgccctgatt cgcttaacgc taccaatgcc atgaagcctc gctcgaagac cgtttctgct
     2041 gagttgtatg ctcttttacg tgcggcataa tcagcttgca tttgtgcgat gatcgcttca
     2101 cgctcaattt tctgctgttc gtacaatgcc atcgcttgcg gatcggatgg attggcaggc
     2161 ttatccggtt caggcggaag attgggctgg ggtggtgggg cgcttgggtc aacttcaccg
     2221 atcaaagtgt cgttggtgta aatccaaaaa tgatttccac gccctacgat tgtcagacgg
     2281 ttggttgtgt cattttccca tctgaaactt ttgtctttat atcttgcata aatatcccta
     2341 cctgttacaa cttcgccatc tgacatggtg gaaaaaacaa aatgtccact tgctacacgg
     2401 gtaatgactg ccacatattg atccaatgca tttgtattgc cgtcagacct catgacaaac
     2461 ccgcagcctg aaatactgcc gacagtattc caagtaatgt cagaagatat tacaaagtct
     2521 ttcgccagtg taccaatata ataatttatg tattcaaatt ggtgataacc agatgcgtcc
     2581 agcgatactg gcggatggat ccagccgatc ttaccctctg aaggatcaac gccatatttg
     2641 ggcaggctct ccagaattgg agcaaatgcc actgctgttg ctgtcaatga atctgaattc
     2701 gtttcaacaa ctggctgggt gccctgtgaa ctttgtgctg ctgccgctgt accagttgca
     2761 cggacttggg cactaattgc ctcaacagta ctttctcttg aggctgtgct gccgctggtt
     2821 ccacaagcca acgagattag cataaagatc aacgggaata ttatgagtct attttttcgc
     2881 atcgggcact aaatcacatc tttacgtttc tgcagatata aaatagcaac aaacaataag
     2941 acaataatga aactttgcac ggtccaggtc aatataacct tggtgtgata tgccaacggg
     3001 tcatctttat caacatatgc gaaacctgat ccattgacaa aggtccggac aacggcttcg
     3061 gcagttgcaa cttgcacctt tagctggctg attactgtct gataggcgac ctgttcttct
     3121 tgatacttgt caacctttgc tttataaagc tcaacttcag cttcgtagat tttgagtttg
     3181 gcaaagaaat ctgccatggc aatattgtct gattgatctt gtggctcaac agggcgaaca
     3241 ggttcaggcg gtaatggcgc gggtgggatt ggcaatggtt gatccacaag cgggatgtag
     3301 aacttgccga tgccaggatg attacaagat tcctttttaa gcaggtttac acccatgcat
     3361 ttgcaattat tggcaagctt ttcttccaaa gacatcgcat tgcgaacatc aggtggtagc
     3421 gcccaacaaa catctgccgc aacatcagac ccagaccctg agatggcgat cagagattca
     3481 aatgcccaac gtgttgagaa gggggcgctc acaaaacttg gaaccggaat taatgcacca
     3541 gccaatacga tctgcggcag cattaaaatt atgacaagta ggggggcaga actggagttg
     3601 ggcgccaatg ctgaagcaaa caacccaagc atcattccgg caaaggtggc aagagtcatg
     3661 gtgatatata tctggatgaa ctccatcact ccgcccggca ttctaaatga aatatagtgg
     3721 accaccgtat atgccactgc ctgatagagt gccaataaag cagcgaccca tacctttgac
     3781 aatacatacg gaaaaatttt gagattgacc aatcgctctc gccgataaat atcctgttcc
     3841 ttaacgatct cgcgcatttg agaaattccg ccgaccataa tgccgtatat cgttaagagg
     3901 aaaagagtga tcatgacttg agacatgtca cctttataga aatcaaaaat ctcccgcccc
     3961 attacaaaag aaaggattac atctaataaa cttaccactg gcgcagtggc aagcattaat
     4021 cccagcgcaa aacggtcacg ggtcaatatt ttgatattac gagcagatag gatcattaac
     4081 tgtcttaatg aagagacctg tctcgtatta gctgcttttt ggggaagtct ttcgggctgt
     4141 ggtaccgtcg ccacactttg tggtgacaac ttgccagcca acggctttaa gacatattcc
     4201 gtgtgggcgt tacttgagcg atatcggttc gcccaatctt ctgctttgcc tttgctggga
     4261 tcatccaaaa tggcatagat ctcatcgaac tcgatcttgc cggctcggcg agcccgctca
     4321 gaacgatact ggtcaaaata agctagcgct tcatcaggag gaccgaacca tgctaaataa
     4381 cctcctcttg cgagaaagat caccttatct gccagcataa cgttttttgt ggcatgtgta
     4441 attaagatga tggtacgtcc ttggtctgcc aggcgacgca tcaattgcat taacgctgtt
     4501 tctgtgccgg ggtctaaccc tgatgtgggt tcatcaagaa agaacagacc gggtttagtc
     4561 aacagttcaa caccaatcga gacgcgtttt tgctgcccgc cgctgagtcc gctgatctga
     4621 acgtctttgc ggtgggtcag gtcaaggtct tccaaaactt ccataattcg tttgtgccgc
     4681 tcttccgggg aagtgtcggc tggcatgcgc aattgagccg cataatcaag tgcctgatag
     4741 atagtcaact ccatgtgaat gatatctttt tgtggcacga acccaatctc attgcggatc
     4801 gcgtcaaaat gagtgtagat atcaatgtca ttgacgtata cttttgaagg aggagttgct
     4861 ggacggtacc cagcgactgc atccacaaat gtggatttgc ctccgccgct ctgccctaca
     4921 acaacgatga attctctggg gctaaatgat gcatatatgt tttgaagaat attgagatct
     4981 ttgcgaaccc acttattcaa accaactaca tccactctta agccattccc gtcatcatat
     5041 ctggcaagtt gatccttgcc catcacgaat cgatattgac caattcggat ggaatcatta
     5101 ggcttcagcc agactgatcc ttcaattcgt tctccattga cataggttcc attagaactg
     5161 cggaggtctt ctacccgata gcgttgccct actttttcga tctgggtatg gaatcgagat
     5221 acattggggg aggggatgac cacatcattg ctggggtcac gcccgatttg aatgagcgtt
     5281 ttctcgccga agacgatgtc tcgctccatt tgctgagatg cttcactggg cgcattgtag
     5341 gttatagtta ccataacccc agggtccata ctgccaattc tcagcagatc attgtgatgg
     5401 agaacatgtg agccgtctat cggacgccct tcaaattgaa ccggattttt tgcttcaggc
     5461 atcaccacca acttgtaccc gccgcccttg acctgctcaa gccgcgcatg ccggcttgag
     5521 acaatctgag atgggatgac aatgtcgttg gtgtcgagtc tgccaatgga tatgctggaa
     5581 cctgttaacg ggtaggtttg tgtgcttcct ccggcaatac taacgattag ctggcgtgga
     5641 ccagcacttg cttgcggtat tgctggtgct tcaccaagca cagtttgcat cacatgtgca
     5701 ggtttagcag gaccggaatg tttggcagtt ttgttcgcgt cttttatgct gacaacagtt
     5761 aggttggagt cttcttcagg aacgggagcg gtgtaaacca ggctgataat ttgccccaat
     5821 cgaattttgt cacctgattt tagtgtgtgt tgcttatcta ttttttcccc attgacgtat
     5881 gtgccattac tgcttcccag atcttccagt acataatttt cgccaatcaa catcagtctg
     5941 gcatgacggc gtgacacagc agatgcgggt atggtgatat ccacactgtc ttcacgacca
     6001 atgatgattt ctgactttgt taattcaaat tctttggaag gtgtactgct ttgatcattg
     6061 ataaggctta gtttgaaact gttcacttga gtcaaaggat gatcctttct tgcaaatttt
     6121 caacacagtt tcttgaattt atggataagt gagaatttgt acagaattga gataattaag
     6181 acaagattat acctttttat taaaatgcat gtcaacctac caatggggga t
//
LOCUS       DAOQJE010000077         6211 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_7129_length_6211_cov_4.873944, whole genome shotgun sequence.
ACCESSION   DAOQJE010000077 DAOQJE010000000
VERSION     DAOQJE010000077.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 6211)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6211)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6211
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7129_length_6211_cov_4.873944"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..280
                     /locus_tag="PLT08_03235"
     CDS             <1..280
                     /locus_tag="PLT08_03235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE03507.1"
                     /translation="VSYYYPGPWISYRPYYSDGEHTQDLLNNLRASQYLIVYYANQGQ
                     LAHHKNFVEAMSKAKPFHVIWLNGYEYVRIYKVAELGPEIYDALSHLK"
     gene            277..2007
                     /locus_tag="PLT08_03240"
     CDS             277..2007
                     /locus_tag="PLT08_03240"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HNE03508.1"
                     /translation="MNNFLKLHLRKIQFALLLLIVSLPSRFINIDRAVNIDEPWWVIS
                     SSNFYYAVTHRDFKDTYFEYHPGVTNMWVISTAMHMYFPEYRGYGQGFFDQRKPFFEE
                     FLREHGKETLDLVRNARYIQAVILTLLALIAFFLLSFLVGENAAFLSIALAVISPFFL
                     GHSHLLNMESMGALFVLVSLLGMHVYLSQDRKWFFLVLSGGAFGLAQLNKSTSIALLG
                     VISLMLLFQLLKRDNRTFGAKFGGVIRIFGIWFGVAALVYFVFWPGMWVDPARMLREV
                     YGNAFSYAFQGARLDVTEELDPAEFSLVTRGEGVFMYLRYWASGTTFLTWLGILLSAF
                     FLFSKEKERMPAVVKSLIGYLALLGVSFILMFGIAQGRNHAHYIMNTFVAFDVIAGIG
                     WGSALLWVGARWKGLASTTASVVVLVVITLAQIGFGLPYAPYYFTYKNPFAKEAATYG
                     YGEGFSEAADYLAQKPNAKELTAYVYNGMGTFSYFFPGKTVVLKRANVLYGDFVTITD
                     ELRSSDYLVLYPIVREKQPETEKIFSELEGVVKPEKVIYINGLEYIQIYKVTDIPETV
                     YEKLLAKNEP"
     gene            2188..6180
                     /locus_tag="PLT08_03245"
     CDS             2188..6180
                     /locus_tag="PLT08_03245"
                     /inference="COORDINATES: protein motif:HMM:NF024583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HNE03509.1"
                     /translation="MNFNSSNGRFSEKSIGRVLMGIVFLYQAIAVVVVVWALVVAAQW
                     VREPFLGSFYEHTLVFTDPAADSTGVWAFNKIVNSGDQLIAINGTDVKNSRDVREVLS
                     SFVPGENVTATVKFKNGETRDLSVELQRFPEDAVSIYLILPSIISFVFLVLSIWTFGL
                     RRNEPSGRAFALFASSFAIVTGTIFNLWTSHEFTTIWTAAVAIAGGANIALALSFPAS
                     PRLIINRPYLRWMGFVLSFVLTAITIPYLFNSQNPTEYIAKWQNLYYFAAVGILSLLG
                     SNLYYSLYSQSPVVKLQARTTLIGAVFAFLPLTVYLVFGNIFSIKFSPWLFLPVVIFP
                     AIMGYNIMRFRFLRTDDLARRGVMYVLLTAIVTMGYALVAAGAAFLFGNSLPQNYLAG
                     FFILVLAIVLEPLRIRLQNLTDVLFFRGSRVVAEQLEDFSHKLTTALDLNAISSLLRD
                     QIASTLAPDQIHIFTYDSLNDFFVASPGDGRRTTTDIRFTSASPLVSYFRSERLPLYL
                     DNGTDLPDVLKPEQSRIALLGARLFIAFPGKDKPNGWLALGPRLTNQAYSPSDLRFLE
                     NISDQASVAIERVQTVAHLERRIQEMNALTRVSQGVNVTLTFDDVLELIYAQTAQIVP
                     TSHFHITLYDKDSDYYYYGFCLENNERMHERENQPFSSNVGLSPDVIRKSRPIITQDY
                     IRECQARNHTPTIENIFAWIGVPLNAGAESIGALSVGSRDASTIYTRAQVDLLQAIAD
                     QTAGAIVKARLLKETQERAHQLTTLNEITRQLTSTLELNPLLQNILENAVGILNCEAG
                     SLLLVDDQTDELVFRVTVGPVAGNLIGKRLARGTGIVGRAVETRKPVIENEEQTSNRF
                     NGVDQQTGFVSRSLLAVPLQAKDRVIGVVEVINRRDGLPFVQNDQTLLTAFAGQAAVA
                     IENARLYTLTDQELANRVEELSVMQRIDRELNASLEMDRAMRITLDWALRQSGADAGL
                     IGTLEETKLRVMAHQGLDEQMASLPDQVMKIELPSMLAAVETGSPQNVEVASGASKNK
                     LLMASQTQMVIPIRRETMVIGLLYLESSSGSQVDIDFLTRLTDHAAIAISNAQLYSEV
                     QRANLAKSDFVSFVAHELKNPMTSIKGYTELLASGAVGQITEMQTNFLGTIKSNVERM
                     STLVSDLNDNSKIEAGRLRLEFKATQASDLVDEVIRTFKRQLEDKKQTLELSIPPNLP
                     AMWADRIRVGQVLTNLVSNAFKYTPESGAIQVGVEESPNHWDANGAPRVIHLWVKDSG
                     IGMTPEDQQKIFQKFFRSDDPKAREAPGTGLGLNITKSLVEMQGGQIWFESEYRHGTT
                     FHFTVPVAEE"
BASE COUNT         1476 a         1347 c         1549 g         1839 t
ORIGIN      
        1 tgtttcgtat tactacccag gcccatggat tagttaccgt ccatactatt cggatggaga
       61 acatacacaa gatttattga ataacttacg tgcttctcag tatttgattg tgtattatgc
      121 gaaccaagga cagttagcac atcacaaaaa ttttgtagag gccatgtcga aggctaagcc
      181 tttccatgtt atctggttaa atggatatga atatgtccgt atctataaag tggctgaact
      241 tggccccgag atatatgatg ctttatcaca cttaaaatga acaatttcct gaaacttcat
      301 cttcgtaaaa tccagtttgc tttacttttg ctgatagtat cccttccttc gcgctttatc
      361 aatatagatc gtgcagtcaa tattgatgag ccgtggtggg tgatttctag ttccaatttt
      421 tattatgcgg tcacgcatcg tgattttaaa gacacatatt ttgaatatca ccctggtgtg
      481 acgaatatgt gggttatctc tactgcaatg catatgtatt tccctgaata tcgtggttat
      541 ggacaagggt ttttcgatca acgtaagcca ttctttgaag aatttttgcg ggagcatggc
      601 aaagagactt tagaccttgt ccgcaatgct cgttatattc aggcagtgat attgacattg
      661 ctggcgttga tagcattttt cttgcttagt tttcttgttg gggaaaatgc agcttttctc
      721 tcgattgctt tggcagtgat ctctccattt tttttaggac attctcattt gctaaacatg
      781 gagagtatgg gggcgttgtt tgtgttggtt tcgttattgg gtatgcatgt atacctttct
      841 caggatagaa agtggttctt tcttgtgctt tctggcggag catttggact ggcacaattg
      901 aataagtcca cttcgatcgc gttgttaggt gtgatcagct tgatgcttct atttcagttg
      961 cttaaacgtg acaacagaac ttttggcgca aagtttggcg gagtgattcg tatctttggg
     1021 atttggttcg gtgtagcggc tttggtgtat tttgtcttct ggccgggtat gtgggtggat
     1081 cctgctcgaa tgttgcgcga agtctatggt aatgctttta gttatgcttt tcaaggtgct
     1141 cgtcttgatg taaccgagga attagaccca gcagaattta gtttggtcac ccgtggagaa
     1201 ggcgttttta tgtatctccg ctattgggcg tcggggacaa cctttcttac ttggttggga
     1261 atcctattgt ctgcattctt tcttttctct aaagagaaag aaagaatgcc tgccgttgtt
     1321 aagtccttga ttgggtattt ggcgttgttg ggtgtctcat tcattttaat gtttggcatc
     1381 gcacaaggac gtaatcacgc tcattacatt atgaacacct ttgtcgcatt tgatgtcatt
     1441 gcgggaatcg ggtgggggag cgcattattg tgggtgggcg cgcgctggaa aggactggcg
     1501 agtacaactg cgtctgttgt ggttttggtt gtgattacgc ttgctcagat tgggtttggg
     1561 ttgccgtatg cgccttatta ctttacgtac aagaatccgt ttgctaaaga ggcggcgaca
     1621 tatgggtatg gcgagggttt ttctgaggca gcagattatt tggcacaaaa gccaaatgca
     1681 aaagagttga cggcatatgt ctataacggt atgggcacct tctcgtattt cttccctggg
     1741 aagaccgtcg ttttgaaacg cgccaatgtt ttgtatggtg actttgtgac cattactgac
     1801 gaactacgaa gctctgatta tttggttttg tatccgattg ttcgagaaaa acagccggag
     1861 acagaaaaaa tcttcagcga gttggaaggg gttgtaaagc ctgaaaaggt catctacatc
     1921 aacggattgg aatatatcca aatctataaa gtgaccgata ttcccgaaac agtgtatgag
     1981 aagttattgg cgaaaaatga accgtaagat attaaacggc gcgatgagcg ccgttttgct
     2041 tgggctacag agtaggggat tttaggatgg gagaaacagg caaaagtcca gaaagaggcg
     2101 ggttttccta tcccacctta cgcctttgtc atgaaagtac catatgattt gggcgggatt
     2161 gtagagtaaa atttcagtca ttaagctatg aatttcaact catccaacgg tcgattttct
     2221 gaaaagtcca tagggcgcgt cctgatgggg attgtgttct tgtatcaggc gatagctgtg
     2281 gttgtcgttg tttgggcgct ggtggtggct gcacagtggg tacgtgagcc tttcttgggt
     2341 tctttctacg aacatacact ggtttttaca gacccagcag ccgactcgac cggggtttgg
     2401 gcattcaata aaattgtaaa tagcggcgat cagttgattg ccatcaatgg gacggacgtt
     2461 aagaacagcc gtgatgtgcg tgaagtactt tcaagttttg tgcccggcga aaatgtaact
     2521 gcaactgtaa aatttaagaa tggcgagact cgcgacctga gtgtggagtt gcagcgcttc
     2581 cctgaagatg cggttagtat ttatctcatt ctcccgtcga ttatcagttt tgtttttctt
     2641 gtccttagta tttggacttt tggtttacgt cgcaatgaac cttctggtcg tgcgtttgct
     2701 ttatttgcat cctcttttgc aattgtcacc ggaacgatct tcaacttgtg gaccagccac
     2761 gaatttacaa ccatatggac ggcggctgtt gctattgcgg gtggggcaaa tattgcgttg
     2821 gctttatcgt ttccggcttc accaaggctt attatcaatc gtccttatct ccgttggatg
     2881 ggttttgttc ttagttttgt tttgacggcg attacgatcc catatctttt caattcacaa
     2941 aaccccaccg agtacattgc aaagtggcaa aatctttact actttgcggc tgttggtatt
     3001 ttgtcgctcc ttggatccaa tttatattat tcactgtatt cacaatcgcc tgttgttaag
     3061 ctacaagcac gcactacatt gatcggcgcc gtcttcgcat ttttgccgtt gactgtctat
     3121 ttggtcttcg gcaatatttt ctccatcaag ttttctccgt ggctgtttct gccggttgtg
     3181 attttccccg cgatcatggg ctacaacatc atgcgcttcc gcttcttgcg tactgatgat
     3241 cttgctcggc ggggtgtgat gtatgttttg ttaactgcaa tcgtcactat gggctatgcc
     3301 ctagtagctg ctggtgctgc gttcttattt ggaaactccc tgcctcagaa ttatctggcg
     3361 ggctttttca ttttggtgct ggcgatcgtt ttggaaccgc ttcgcatccg cctccaaaac
     3421 ctgacggatg ttctattctt ccgtggttcg cgggtggtgg cagaacagtt ggaagacttc
     3481 tctcataaac ttaccaccgc tctagatttg aatgccatca gttcgctcct gcgggatcag
     3541 atcgcctcaa ccctcgcccc agaccagatt cacatcttta catacgactc cctcaatgat
     3601 ttcttcgtcg cctctcccgg cgacggacgc cgtactacga ctgatattcg ttttacatct
     3661 gcaagcccat tggtgagtta cttcaggagc gaacgcttgc cgctgtattt ggacaatggt
     3721 accgacctgc ctgatgtgct taagccggaa cagtcgcgta ttgcgttgtt gggtgcccgt
     3781 ttgtttattg cattccccgg taaggataag ccgaatggct ggctggcttt gggtccacgc
     3841 ctgacgaatc aggcatattc tccaagcgat ttgcgcttcc ttgaaaatat cagcgaccaa
     3901 gcatccgttg ctattgaacg cgtgcagacg gtggcgcact tggaacgccg aattcaagaa
     3961 atgaacgcgt tgacccgcgt ttcacaaggc gtaaacgtta cgctgacctt tgatgatgtc
     4021 ttggaattaa tctatgctca aaccgcacag atcgttccaa cttctcactt ccatatcacg
     4081 ctatatgata aagacagtga ttattactat tatggtttct gtcttgaaaa taatgagcgt
     4141 atgcacgaac gcgaaaacca acctttctcg tccaatgttg ggctttctcc agatgttatc
     4201 cgcaagagcc gcccgatcat tactcaagat tacattcgtg aatgtcaggc gcggaatcac
     4261 actcccacca tcgaaaatat ctttgcatgg atcggtgtgc cgcttaacgc tggagctgaa
     4321 tcgattggtg cccttagtgt tggtagccgt gatgcctcca cgatctatac tcgcgctcaa
     4381 gtagacctat tgcaggcgat tgcagaccaa accgctggtg caatcgttaa agcgcgcttg
     4441 ctgaaagaga ctcaggaacg tgcgcatcaa ctgacaacct tgaatgagat tacgcgtcag
     4501 ttgacttcga cgcttgaatt gaatccgctt ttgcagaaca ttttggaaaa cgctgtaggc
     4561 attttgaact gcgaggcagg gagtttgttg ctggtcgatg atcaaacgga tgaactagtt
     4621 ttccgcgtaa cagtaggacc tgtagctggc aaccttatcg gcaagcgtct tgctcgcgga
     4681 acgggcattg tggggcgggc ggttgaaacg cgcaagcctg tgattgaaaa tgaagagcag
     4741 acgtcgaacc gcttcaatgg agtggatcaa caaacggggt ttgtgagccg ctcgttactc
     4801 gccgttccac tgcaagcgaa agaccgcgta attggcgtgg tggaagtcat caaccgccgc
     4861 gatggtctgc cctttgtaca gaatgaccaa accttgttaa ctgcgtttgc gggtcaggct
     4921 gcggttgcta tcgaaaatgc gcgtttgtat acccttacag atcaagaact tgccaatcgc
     4981 gtagaagaac tttcggttat gcagcgcatt gaccgtgaat tgaatgccag ccttgaaatg
     5041 gatcgtgcca tgcgcatcac actcgattgg gcgttgcgac aatctggtgc agatgctggt
     5101 ttgatcggta cgctcgaaga aacaaaattg cgtgtgatgg cacatcaagg tttggacgaa
     5161 caaatggctt cgctacctga ccaggtgatg aagatcgaat tgccgtccat gttggcggct
     5221 gttgaaacag gttcaccgca aaatgtggaa gtagccagcg gggcttcaaa aaataaacta
     5281 ctgatggcat cacagactca aatggtgatt cccatccgtc gtgaaacgat ggtgattggg
     5341 ttgctatatt tggagagttc cagcggctcg caagtggata tcgacttttt gacccgcctt
     5401 accgaccatg ccgctatcgc gatctcgaat gctcagctgt atagcgaagt gcaacgcgcc
     5461 aaccttgcaa aaagcgactt cgtctctttc gtggcgcatg agcttaagaa cccaatgacc
     5521 tcgatcaaag gctataccga gttattagcc agcggggcgg tgggacagat caccgaaatg
     5581 caaaccaact tcttgggtac gatcaagtcg aatgtggagc gtatgtccac attggtatcg
     5641 gacctcaacg ataattccaa aatcgaagcg ggtcgcctac gcttggaatt caaagccaca
     5701 caagcttctg atttggtgga tgaagtcatt cgcaccttta aacgtcaact tgaagataag
     5761 aagcaaaccc ttgaattaag catcccacca aacttgcctg ccatgtgggc agaccgcatc
     5821 cgcgtgggac aggtgttgac caaccttgtt agcaatgcct tcaaatacac accagaaagc
     5881 ggcgccattc aagttggtgt agaagaaagc ccaaatcatt gggacgccaa tggggctcca
     5941 cgtgttatcc atttgtgggt gaaagacagt ggtattggta tgacacctga agatcagcag
     6001 aagatcttcc agaaattctt ccgttccgat gaccccaagg ctcgcgaagc gccaggcaca
     6061 ggtctcggtt tgaacattac caaaagcctt gtagaaatgc agggcgggca gatttggttc
     6121 gagagtgaat atcgacacgg cacaaccttc catttcactg tgccagtggc agaggagtaa
     6181 tcagcgatgg cgatagtctc ttctgttgga a
//
LOCUS       DAOQJE010000078         6183 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_7175_length_6183_cov_5.259628, whole genome shotgun sequence.
ACCESSION   DAOQJE010000078 DAOQJE010000000
VERSION     DAOQJE010000078.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 6183)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6183)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6183
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7175_length_6183_cov_5.259628"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(108..602)
                     /locus_tag="PLT08_03250"
     CDS             complement(108..602)
                     /locus_tag="PLT08_03250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE03510.1"
                     /translation="MKKIYITTKSLWQIFLVLVEVFGITSLLVYASQSLQPTQNGFDV
                     VERYITFIAAYEILVYIILTSLNDIRRDSLLALRTSFEQALFYCETGSDFAKDKLVEK
                     IAKQLENSTFNHLDVRKEYKNLLQYIEAKHELAIKYALLNINHNYEMCDLQWKFTFLL
                     RWFK"
     gene            complement(843..3785)
                     /locus_tag="PLT08_03255"
     CDS             complement(843..3785)
                     /locus_tag="PLT08_03255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE03511.1"
                     /translation="MANKITVKQFIEKWSKMELRERASSQSHFNDVCALIGHATPTDI
                     DPTGEFFTFEANVDKDTEKKHGWADAWYKGKFIWEYKGPHKNLDVAYQQLLRYRESLG
                     NPPLLITSDTQKIIIHTNFTNTAKRIEVIDFDRLQKDGLQLLENAFHNPDAFKPSETQ
                     EKITEKTADAFVSFARDLQEWGKRTYHPERLAHFVVRILFCLFAEDIGLLPDKVFTEL
                     IRVRYKDQTTEQFVKSLRILFAAMKNGNSTFGAHVIPWFNGGLFEDDFVPEMPGGTLH
                     ELLAACDKDWASIDPSIFGTLFERVIDESKRAQLGAHYTSKDDIMLVVEPVLMTPLRT
                     KWRKIKAEVENQIKQGIPSAAFVTLKKFSDEIASIRVLDPACGSGNFLYIALRELLDL
                     QKQIIVFAEEHSLSEIRLTVSPQQLFGLELNPYAHQLAQITAWIGYIQWRYDNGFADI
                     SEPILKELKQIQNRDAIIYTNLSGGETKGIKAVAESIWDEVDVIIGNPPFLGSRKMRP
                     ILGDEYCDALLKVYGKQIVGMPDLVCYWFEKARQQVANGKAKKVGLLGTQAIRGGTNR
                     QVLDKIKDSGDIFMAWSDKEWILDGATVHVSIVGFDDGTEDNRMLDGLPASSINSDLT
                     SDVDISEGYILPENTSLSFQGVVLRGQFDMPHSKAEEMIKRSNNPNGHSNSDVLKPRR
                     TGDDVVNRNSDTYVVDFGVDMPMDKAAEYVAPFEYLKENVYEMRQKANQAVAREKWWI
                     HWNARTQMREALSNLSRYIATPRVAKHRIFAWLDGSILPDAQLVVFAREDDYFFGVLH
                     SKVHEIWSRRKGTQLRDAESGFRYTSTTTFETFALPWRPSEEPEKDPRYQAIAHSAKS
                     LNEFRSNWQNPSDEYIRGSTAFTKRTLTNLYNCLELYRDKIKGKRRSEEIWRQEMLLL
                     FKVKASAKKQLPVTLIVSLDEIETLDSLHTELNNAVLDAYGWSRTISDENILESLLKL
                     NLERAGIK"
     gene            complement(4061..>6183)
                     /locus_tag="PLT08_03260"
     CDS             complement(4061..>6183)
                     /locus_tag="PLT08_03260"
                     /inference="COORDINATES: protein motif:HMM:NF014442.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006306 - DNA methylation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="N-6 DNA methylase"
                     /protein_id="HNE03512.1"
                     /translation="TIDRIVFLRICEERGIEPENQLLEIANNENSYEELCELFKRADT
                     KYNSGLFHFKNEKEVVSRPDGLTLSLAIDDKVLKDIISNLYYPKSPYAFLYIPSDILG
                     QVYERFLGKVIRLTAGHSAKVEEKPEVRKAGGVFYTPTYIVEYIVKNTVGKLVENKSP
                     KDVALLKIVDPACGSGTFLLGAYQFLLDWHLNWYSENEPEKAEKNKAIVKVGDGYRLT
                     TAKKKEILLNNIYGVDIDAQAVEVTKLSLLLKVLENASGQLGLGMERVLPDLGNNIKC
                     GNSLVGFNYFEGQLFPDETEQTNINPFNWQSEFKEAFAKGGFDVVIGNPPYAYRNATE
                     DNLREYYLKSYQSAEGNFELYKFFIENNLRINREKGLSGIIVSATFLVQPSFQKLRKL
                     ILSNSNIIELSPLGPGVFEDATVDTTILVLEKASEIQNHKVGIKTPKPPVNLAITSPY
                     FVSQDRFAQNSGMVFDYKLSDISANVTQKLFENFPNIESGFEFGVGINTGFIRDELVS
                     DKKEDKRYHPMVAGNGISRYGKVNTKGWIMYDKEYVKKRGKLGRTLPAENLLSSEKIL
                     VVRTRNLSLQKRVIATIDSDGCYNLNRLSNIVARDGYNLFGLLGILNSSLFNWLFSTR
                     FYDYEIKPVYLRNSPLADCNNNKLVSLVEQIINLNKNLVSAKTPQEKESLERKIEVTD
                     KAIDSLVYELYGLNDEEIKIVEGQ"
BASE COUNT         1770 a         1316 c         1123 g         1974 t
ORIGIN      
        1 cttgagattg ccgcagaatc cccagcgtca ggtgcacgct ttgttggctg gctttgtgca
       61 agacaccttt gctataaaaa taacttactc aatattacca acaaaaatca cttaaaccaa
      121 cgcaagagga aagtaaattt ccattgtaaa tcgcacattt cgtaattgtg attaatgttt
      181 aataacgcgt atttgattgc taactcgtgc ttggcttcaa tatattgaag aagattcttg
      241 tactcttttc tgacgtcaag atgattaaag gtgctgtttt ctaattgctt ggctattttt
      301 tcaaccaact tgtcttttgc gaaatctgag ccagtttcac aataaaagag tgcttgttca
      361 aaagatgtcc tgagcgctaa aagagaatca cgccgaatat catttaacga tgtcaagatt
      421 atgtatacaa ggatttcata agcggcaata aatgtaatgt agcgttcgac aacatcaaat
      481 ccgttttgag tgggttggag agattgactt gcataaacca agagagatgt tatcccaaaa
      541 acttcaacaa gaaccaagaa aatctgccac aaggatttcg ttgttatata gatttttttc
      601 atttacacct ttaggcgata ttattgaaaa ggttcgtatg ttttcgctct ttggcgtcag
      661 ccagccaacg gtttgcgtta cctgcgtgtg ggcgggcgtg gattctgctt gggagcagga
      721 aaaacccgaa gccagaaaaa tgcttgaaaa tgccgcagaa tcccacacgt caggtgcacg
      781 ctttgttagg cttcgtcctt gctataaaga ctctattctt tctggacggc gcaaattaaa
      841 cactatttta ttcctgctcg ctccaaattt aatttcagca aactctcaag aatattttca
      901 tcgctgattg ttcttgacca accgtaggca tctaaaactg cattgttaag ttccgtgtgc
      961 aggctatcaa gagtctctat ctcatctaaa gaaacaatca gagttacagg aagttgtttt
     1021 tttgcactag cctttacttt gaatagcaaa agcatttcct gtcgccaaat ttcttctgaa
     1081 cgtcgttttc cttttatctt gtctcgatag agttcaagac aattatacaa atttgtcaaa
     1141 gtgcgtttag taaatgcggt tgagccacgt atatattcat cagacgggtt ttgccaattt
     1201 gacctaaatt cattcaaaga ctttgccgaa tgtgctattg cttgataacg tgggtctttc
     1261 tctggttctt cgctaggtcg ccaaggaagt gcaaaagttt caaatgtagt ggtagatgta
     1321 tatctgaaac cactttctgc atctctcaat tgtgtacctt tacgccttga ccaaatttcg
     1381 tgaacctttg aatgcaaaac accaaagaaa taatcatctt ctcgtgcaaa tacaaccaac
     1441 tgagcatcag ggagtatgct accgtcaagc catgcaaaaa ttctgtgttt ggctactctg
     1501 ggtgtagcaa tataacgtga aagatttgac aatgcttcac gcatttgtgt tcttgcattc
     1561 caatgaatcc accatttttc acgagcaacc gcctgatttg ctttctggcg catttcatag
     1621 acattttctt tcaaatactc aaatggcgca acatattcag ccgccttatc cattggcata
     1681 tcaacgccaa aatcaaccac gtaagtatct gaatttcgat ttactacgtc atcacctgtt
     1741 cttcttggct ttagcacatc actgtttgag tgaccatttg gattgtttga acgttttatc
     1801 atttcttcgg ctttgctgtg cggcatatca aattgaccac gcaaaacaac gccttgaaat
     1861 gataggcttg tattttcagg gagaatatat ccttctgaaa tatcaacatc tgatgtcaag
     1921 tcagagttaa tggaagatgc tggcaaacca tcaagcattc tattatcttc tgtgccatca
     1981 tcaaacccaa caattgacac atgaacggtt gcgccgtcca aaatccattc tttgtcgctc
     2041 catgccatga aaatatcgcc agaatctttt attttgtcga gtacttgtct atttgtgccg
     2101 ccacgaattg cttgtgtccc aagcaatccc accttttttg cttttccatt cgcaacttgt
     2161 tgacgggctt tctcaaacca atagcatacc aaatctggca tacccacgat ttgctttcca
     2221 tataccttta aaagtgcatc gcaatattca tcacctagaa tcgggcgcat tttcctgcta
     2281 cccaagaacg gaggattgcc aatgatgaca tctacttcat cccatattga ctcggcaacc
     2341 gcttttatgc cttttgtctc tccgccagaa aggttagtgt atatgattgc atcacgattt
     2401 tgaatttgtt ttagttcttt caaaatcggt tcgcttatat ctgcaaaccc attatcgtat
     2461 cgccattgaa tataaccaat ccaagccgtg atttgtgcca gttggtgagc atacggatta
     2521 agttctaacc caaaaagttg ttgtggcgaa actgtgagcc gaatctccga aagactgtgt
     2581 tcttccgcaa aaacaataat ctgtttttgt aggtcgagca attctcgtaa agcgatataa
     2641 aggaaatttc cacttccgca agcagggtca agaactcgaa tagaagcaat ctcgtccgaa
     2701 aatttcttca aagtgacaaa ggcggcggaa ggaatgcctt gcttgatttg attttctact
     2761 tctgctttaa tttttcgcca ctttgtacgt aatggagtca ttagcacagg ttcaacgaca
     2821 agcatgatgt cgtctttgct tgtgtaatgc gctccaagtt gagcacgttt ggattcatct
     2881 ataactcttt caaaaagagt tccaaaaata gaagggtcaa tacttgccca atctttatcg
     2941 caagcagcca ataattcatg aagagttcca cctggcattt caggaacgaa atcatcttca
     3001 aataaaccac cgttgaacca cggaatcaca tgagcgccga aagttgagtt cccatttttc
     3061 attgccgcaa aaagtattcg taggctcttg acgaattgct ctgttgtttg gtctttatac
     3121 cgaacccgaa tgagttcagt aaaaactttg tctggcaaca atccaatatc ttcggcaaac
     3181 aagcaaaaca agattcgtac aacgaagtgg gctagtcttt caggatgata agttcgtttg
     3241 ccccattcct gtaaatccct tgcaaaactt acaaaggcat cggcggtttt ttcagtaatc
     3301 ttttcctgtg tttcagatgg cttgaaggca tcagggttat gaaaagcatt ttcaagaagt
     3361 tgtagaccgt ctttctgtaa acggtcaaaa tcaataactt caattcgttt tgctgtattg
     3421 gtaaagttcg tatgaatgat gattttttga gtatccgaag taatcaaaag tggcggattg
     3481 ccaagtgact ctcggtagcg aagtaattgt tgatatgcaa catctaagtt tttatgaggt
     3541 cctttatatt cccaaataaa tttgccttta taccaagcat cagcccatcc atgcttcttt
     3601 tctgtgtctt tatcaacatt ggcttcaaaa gtgaaaaatt cccctgttgg gtcaatgtca
     3661 gttggagttg cgtgcccaat taatgcacaa acatcattga aatggctttg agaagaagca
     3721 cgctctctca actccatctt gctccacttt tcaataaact gctttacagt aattttgttt
     3781 gccattgctt atgatttcgc ctttttatat tgcgtgaagt atatcccact catcgaaatt
     3841 tatctcataa agaattagac atgaaccgaa agaagaagcc taacggtttg cgttacctgc
     3901 gctggggcgg gcggcggaac gccgtccgat tggaaaaatg ataaggcgta gaaaaatgct
     3961 tgaaaatggc gcagaatccc cagcgtcagg tgcacgcttt gttagacccc gcccttgacc
     4021 tgcaagactc tttgccatta aaagacctga acccatttta ctactgacct tccacgattt
     4081 ttatttcttc atcattcaac ccgtataact cataaacgag tgaatcaatt gccttatccg
     4141 tgacttcaat ttttcgctct aaactttcct tctcctgtgg agttttagcc gaaactagat
     4201 ttttgtttag atttattatc tgctccacaa gggaaacaag tttattgtta ttgcaatctg
     4261 ctaacgggct atttcgcaaa taaacaggct ttatttcgta atcgtaaaaa cgtgtagaaa
     4321 atagccagtt gaataaactt gaatttaata tgccaagtaa cccgaataaa ttgtagccat
     4381 ctctggcgac aatatttgaa agcctattga gattgtaaca accatcagaa tcaattgtag
     4441 caatgaccct tttttgtagt gaaagatttc gtgttctgac cacaagaatt ttttccgatg
     4501 aaagcaagtt ttctgcgggt aaagttctac caagttttcc acgttttttc acatactctt
     4561 tatcgtacat aatccaccct ttggtattta ccttcccata acgtgaaata ccgttacccg
     4621 ctaccattgg atgataccgt ttatcttctt ttttatcaga aactaattca tcccgaataa
     4681 aaccagtatt gattcctact ccaaattcaa agcctgattc tatattcggg aaattttcaa
     4741 aaagtttttg agtgacattt gcgcttatat cagataattt gtaatcaaaa accattccgc
     4801 tgttttgtgc aaatctatct tgactgacga aataaggaga ggtgattgcc aaattgacag
     4861 gaggcttcgg ggttttaatt ccaactttgt gattttgaat ttcacttgcc ttctctaaaa
     4921 caagaattgt tgtgtcaacc gtagcgtctt caaatacgcc aggtcccaac ggtgataatt
     4981 caattatgtt tgagtttgac aaaatgagtt ttcgtagttt ttgaaaagaa ggctgaacca
     5041 aaaaagttgc tgaaactatt atgcctgaca aacctttttc tcggtttatg cggagattgt
     5101 tttcaatgaa aaatttatac aactcaaaat taccttctgc tgactggtaa cttttcaaat
     5161 aatattcacg aagattatct tctgttgcgt ttcgataagc ataaggcgga ttgccaataa
     5221 ctacatcaaa accacctttt gcaaatgctt ctttgaactc gctttgccaa ttaaatggat
     5281 ttatgtttgt ttgttctgtt tcatcgggga ataactgacc ttcaaaataa ttgaacccaa
     5341 ctaacgaatt tccgcactta atattatttc ccaagtcggg caaaactcgc tccatgccca
     5401 aacctaactg accgctggca ttttccagca ctttgagcaa aagggaaagt ttggttactt
     5461 ctaccgcttg cgcgtcaatg tccacgccat aaatattatt gagcaagatt tcttttttct
     5521 tcgcagtagt taacctgtaa ccatcgccaa cttttacaat ggctttattt ttctcggctt
     5581 tttctggctc attttctgaa taccagttca aatgccagtc caataaaaac tgatatgcgc
     5641 ccagcaggaa agtacccgaa ccacaagcgg ggtcaacgat tttgagtaaa gcaacatctt
     5701 tcggactttt gttttctacc agttttccaa ctgtattttt gacgatgtat tccacgatgt
     5761 aagtaggagt gtaaaatacc ccgcctgcct tgcggacttc tggcttttct tctacctttg
     5821 cgctatgtcc tgccgttaag cgaatcactt tgcctagaaa acgctcataa acctgcccta
     5881 aaatatcgga aggaatataa agaaaagcat acgggctttt tgggtaatac aagtttgaaa
     5941 tgatgtcttt caaaactttg tcgtcaatcg caagtgaaag agtcaaacca tcgggacgac
     6001 tgacaacttc cttttcattc ttgaagtgaa acaagccaga gttatatttt gtatctgccc
     6061 gtttgaataa ctcgcataat tcttcatacg agttttcatt attggcaatt tccaataact
     6121 gattttcagg ctcgattccc cgttcttcac aaattcgcaa aaatacaatg cggtcaatcg
     6181 tca
//
LOCUS       DAOQJE010000079         6173 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_7195_length_6173_cov_6.027950, whole genome shotgun sequence.
ACCESSION   DAOQJE010000079 DAOQJE010000000
VERSION     DAOQJE010000079.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 6173)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6173)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6173
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7195_length_6173_cov_6.027950"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2247)
                     /locus_tag="PLT08_03265"
     CDS             complement(<1..2247)
                     /locus_tag="PLT08_03265"
                     /inference="COORDINATES: protein motif:HMM:NF024583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HNE03513.1"
                     /translation="MPRTGQKKSAAPKSATKKKAVRATTKKTTSSKPTSVTQLRKELE
                     QREAELAIIKSVQDGLASKLEMQAIYDLVGEKIRDLFKAQTTIIATFDRKTETQSFNY
                     YVDRQGREYIEDRPMSGLVKALIREKKTFLFNEHVEDRMREFNAELVLGPLIPKSALY
                     VPLTTAGEVRGVISLQNMDKEHAFNKSDVRLLETLAGSLCAALENARLLNETQRLLNE
                     TEQRNAELEAIRKATINLSSSLDYTTVLNSLLQNTWTLMPSIEDINLFIYENGELKFG
                     TAIAHGKITNAPAFHPRPGGMTDIVSKSGEMMLVEDMSVDPLYANSNWGGALIGLPLK
                     FSNRVVGVMNIHFAEPRIFTESELRLLKLFGDQAAVVIENSRLFNETERLLSETAGRN
                     AELGVINSLQQVLVSKLDFQSIIDLIGDKVGEIFDAQVTLISLYYPATGEMHHRYINE
                     RGQRMYFDEPMPVDGFRKHVLESKQTLLISHDYLRMAEELGEEPASAGEAPKSLLFAP
                     MVVDGQVTGIMSLQNLDREHAFQESDTKLLSTIAASLSAALNNARLFEETMRHARESE
                     ALNEVGRDISATLNLSAVLDKIAKHARDLLKGSSSAIYLPEPDGKSMRALVAQGDIAE
                     EILADSIQVGEGIIGSLAKLGKAEYINDTNHDPRTLQIPGTPAVQNERLLVTPLLSGE
                     KVNGMMAVWRKGGEPFSPADQRFQEELSLQAAIAIQNANFVNEVEQRAHELEIINSIQ
                     QGLSTKLDV"
     gene            complement(2361..3530)
                     /locus_tag="PLT08_03270"
     CDS             complement(2361..3530)
                     /locus_tag="PLT08_03270"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HNE03514.1"
                     /translation="MNNPPRILIVDDEPFNVDYLEQELADFGYELLTAANGEEALEKI
                     RTGSPDLVLLDIMMPIMDGFTVLGQAKADAAIRNIPIIIISANNDLQSVVKGLQKGAE
                     DYLPKPFEPTILYARISASLDRKRLRDQEQVYLQSLENELNIARNIQQEFLPAELPVL
                     EGWEIAPYFKAAKFVAGDYYDAFTLPNGNLVFIVGDVCGKGIGAALFMTLFRSLLRAT
                     STAGYLVEETATFSPAKHIEQAIHMTNNYVATTHEQALVFSTVFIGILNPTTGALTYV
                     NAGNEAPFLIRNDGTFEELRPTGPVVGAMPGVAYKVREVLLNSGDSLLAFTDGIPDSK
                     NQQDDFYGHERLVEFIKQKDASATALVNRLGKELDLFIDTEEQFDDITILALRKV"
     gene            complement(3542..3895)
                     /locus_tag="PLT08_03275"
     CDS             complement(3542..3895)
                     /locus_tag="PLT08_03275"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNE03515.1"
                     /translation="MKTILIVEDTDLNIDLLTQLLEDDYKLLIAKDGNQGVNMAISKK
                     PDLILMDISLPILDGYEATRQIRKSLPHTPIIGLSAHAMDGHRQKAQDAGCNDYLTKP
                     VNDSLLFDMLAKYLG"
     gene            complement(3908..>6173)
                     /locus_tag="PLT08_03280"
     CDS             complement(3908..>6173)
                     /locus_tag="PLT08_03280"
                     /inference="COORDINATES: protein motif:HMM:NF024583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNE03516.1"
                     /translation="DEHMLAIPLLANDELKGLMAVWRHGADNLFTEAELEFMNNLARQ
                     AVIAIQNTQLFEQTQHLLNQTEQRATELSILNSVSESMTRTLDVTTVTHNVGNKIHEI
                     FNAEIVDILLYDAATNIVQLAYSFSDNIHYEDEPPWELGEGLTSKIIISKQPLLLNTS
                     QEINDHGAAAYLTAPEDEEEIKSYMGVPIMVGDQVLGVVDVQSYKAGAFNEDNLRILQ
                     TLSANMGVAIQNARLFNETQRLFKEAQEARQAAEHANQAKSAFLANMSHELRTPLNAI
                     IGFTRIVRRKSEGLLPDKQTENLDKVLSSSEHLLSLINTVLDIAKIEAGRMDVQAANF
                     NVSSLMDLCANTATPLMKPTVRLVKDVEEDLGMIYSDQDKIKQIVLNLLSNAAKFTAA
                     GKITLDAHRAEDLLVVNVSDTGIGITEEALGRIFEEFQQADTSTTRQYGGTGLGLTIS
                     RNLARLLGGDLTATSEHGKGSTFTLTLPIQYGTKPASSLNPDTAPFHRSGTSTKADAS
                     KKLILVIDDDPDATYLLRENLGPEEFEIIGARNGIIGQEKARELKPDAILLDILMPEK
                     DGWQVLHDLKNDPATTNIPIILLTIVDKKALGMRLGAAAYLLKPLNPAEVLSTLQKVI
                     KPKENARIHVLTVDDDPHIAEMLSQQLPESEFRLESALDGEAGLAAIEAEHPDVILLD
                     IMMPKLDGFGVIERLREKPETRELPIIVISAKELTDDEIARLRESVVLIMRKQGFDGE
                     KLAQEIKHTVQHAN"
BASE COUNT         1485 a         1460 c         1507 g         1721 t
ORIGIN      
        1 cacatccaac ttagtggata acccttgttg aatgctatta atgatctcca actcgtgggc
       61 acgttgctcc acttcgttga cgaagttcgc attttgaatg gcaatcgcgg cttgcaaaga
      121 cagttcttct tgaaaacgct ggtctgcagg ggagaagggt tcgccacctt tgcgccaaac
      181 cgccatcata ccgttgacct tttcaccgga aagcagcgga gttaccagca aacgttcatt
      241 ttgtactgcg ggcgtaccag ggatttgtag cgtgcgcggg tcgtgattgg tgtcgttgat
      301 atattctgcc ttgccgagtt tggcaagcga cccgataatg ccttccccaa cctgaatgga
      361 atctgccaga atttcttctg caatatcccc ttgtgctacc aacgcccgca tcgacttgcc
      421 atctggctcg ggcaaataaa tagcactgct tgagcctttc aataagtcgc gggcgtgctt
      481 ggcgatctta tctaacacgg ctgagagatt taacgtggca gatatatctc gcccaacttc
      541 attgagcgct tctgattcgc gggcatgccg catggtttct tcgaataagc gggcattatt
      601 caacgccgca cttaagctag cggcaatggt agaaagtaac tttgtatccg attcttgaaa
      661 ggcgtgttca cggtccaggt tttgcaagct cataatgcct gtcacctgac catccactac
      721 catgggagca aagagcagcg actttggtgc ttcacctgca gaagcaggct cttcaccaag
      781 ttcttccgcc attcttaagt aatcgtggct aataagcagg gtttgtttgc tttcaagcac
      841 atgcttgcgg aaaccatcca cgggcatggg ttcatcaaag tacatgcgtt gaccgcgttc
      901 attgatatag cggtgatgca tctcacctgt ggcgggataa tacaatgaga tgagtgtgac
      961 ttgcgcatca aagatctcgc cgaccttatc accgatcagg tcaatgatgg attgaaaatc
     1021 cagtttagag accagcactt gttgcaggct gttgatgacg cctaactcgg cattgcgccc
     1081 ggcagtttca gataagaggc gctcggtctc attgaacaaa cgcgaatttt cgatgacgac
     1141 tgcggcttga tctccgaaca atttgagtag gcgtaattca gactctgtaa aaatacgcgg
     1201 ttctgcaaaa tggatattca tcacacccac aacacggttg ctgaatttga gcggcaaccc
     1261 gatcaatgcg ccaccccagt tggagttggc gtagagtggg tcaacgctca tatcttctac
     1321 caacatcatt tcgccagatt tcgaaacgat atcggtcata ccgcccgggc gcggatggaa
     1381 agctggcgca tttgtaatct ttccatgcgc aatggctgtg ccaaacttta attcgccgtt
     1441 ctcatagata aaaagattga tgtcctcaat gctgggcatc aatgtccatg tattttgtag
     1501 caaggaattg agaacggtag tgtaatctaa acttgaagag agattgatgg tcgctttgcg
     1561 gatggcttcc aactccgcgt tgcgttgttc tgtttcgttg agcagacgtt gcgtttcatt
     1621 taagaggcgg gcattttcga gcgcagcaca cagagacccg gcaagagtct caagcaagcg
     1681 cacatctgat ttgttgaagg cgtgctcctt atccatattt tggagggaga ttacaccgcg
     1741 cacctcaccg gctgtcgtca acggcacata caaagccgac tttggaatca acggaccgag
     1801 aaccaactct gcgttgaact ctcgcattcg gtcttcaaca tgttcattaa agaggaaagt
     1861 ctttttctcg cggatcaagg ctttgaccaa cccgctcatg gggcgatctt caatatattc
     1921 tcgtccctgc cggtctacat aatagttgaa cgattgcgtt tctgtcttgc gatcaaaggt
     1981 tgcaatgatg gtggtttgcg ccttaaaaag gtcgcgaatc ttttccccaa ctagatcata
     2041 aatcgcctgc atctccaatt tggaggcaag cccatcttgc acacttttga taatggcaag
     2101 ctccgcctca cgttgttcca gctccttgcg gagttgggta accgaagtgg gtttgctgct
     2161 ggttgttttc ttggttgtag cgcggacggc ttttttcttt gtggctgatt tgggcgcggc
     2221 gcttttcttc tgaccagttc taggcatggt ttcctctgag aatgattcag tttacatagg
     2281 gggcagagaa tcgtcaatag cgtagataaa aaaacaggcg gagaacgcct gtctgtcatt
     2341 caattgttat gttcagacaa tcatactttg cgcaacgcca ggatggtgat atcgtcgaat
     2401 tgttcttcag tatcaatgaa caagtccaac tctttgccaa gacggtttac aagcgctgtt
     2461 gcagaggcat ccttttgttt gatgaattcc accaggcgtt catgaccgta aaaatcatct
     2521 tgctggtttt tgctatctgg aatgccatcg gtgaaagcga gcaagctatc tcctgaatta
     2581 agtaaaactt cacgcacttt gtatgccaca cccggcatcg cacccacaac cggacccgtt
     2641 gggcgcagtt cttcaaacgt cccatcgttg cggattaaga acggcgcttc gttcccggcg
     2701 ttgacatagg tcaacgctcc agtggttggg tttaagatgc cgataaaaac tgtcgaaaag
     2761 accaaggctt gttcatgcgt ggtcgccaca taattgttgg tcatgtgaat ggcttgttca
     2821 atatgtttgg caggggagaa ggtggcagtt tcttctacta aatatcccgc cgtagatgta
     2881 gcacgcaaca aactacggaa caaggtcatg aacaatgcgg ctccaatacc tttcccacaa
     2941 acatctccta cgataaagac caaatttcca ttcggtaatg taaaggcatc atagtaatcg
     3001 ccagccacga actttgccgc tttgaagtac ggagcgatct cccaaccctc caacacgggc
     3061 aactcggctg gaagaaattc ttgctgaatg ttgcgcgcaa tgttcaactc gttttcaagg
     3121 ctttgtaaat agacttgttc ctggtcacgc aggcgtttcc tatccaaact ggcggaaatg
     3181 cgtgcatata aaatggtcgg ttcgaaaggc ttgggtaaat aatcttcggc gcctttttga
     3241 agccccttca ccacgctttg cagatcgttg ttcgcagaaa taataatgat tgggatatta
     3301 cgaatcgccg catcagcttt ggcttgccct aataccgtaa acccatccat gatgggcatc
     3361 ataatatcta gtagtacaag gtctggagat ccagtacgaa ttttctccag tgcctcctct
     3421 ccgtttgcag cggtaagaag ctcataccca aaatcagcaa gctcctgctc taggtaatca
     3481 acattgaatg gttcatcatc tacgatcaaa atacgcggtg gattgttcat aaaagaaagg
     3541 cttatcccaa gtatttggcg agcatgtcaa acagtaaaga atcattcacg ggttttgtga
     3601 ggtaatcgtt acagcctgcg tcttgtgctt tttgtcggtg cccatccatg gcatgggctg
     3661 aaagaccaat gatcggcgtg tgcggaagcg actttcgtat ctgacgtgta gcttcatacc
     3721 catccagaat cggcagcgaa atatccatca ggatcaaatc aggctttttg cttattgcca
     3781 tgttcacacc ctgattacca tctttagcga ttaacaattt gtaatcatcc tcaagtaatt
     3841 gggtgagtag gtcaatgttt aggtctgtat cttcaacgat taatatagtt ttcatacact
     3901 ctccaattta atttgcgtgt tgcacagtgt gtttgatttc ttgtgccaat ttttcaccat
     3961 caaagccttg cttgcgcatg ataagaacta cactctcgcg caatcttgca atttcatcat
     4021 cggtcaactc tttggcgcta atgacgatga taggcagttc acgtgtctca ggcttttcgc
     4081 gcaagcgttc gatgacgcca aaaccatcca acttgggcat cataatatca agcaaaatga
     4141 catctggatg ctccgcctca atcgccgcca aacctgcttc accatccaaa gcggactcta
     4201 gtctgaattc ggattctggt aactgctggc ttaacatctc agcaatatgt gggtcatcat
     4261 ccaccgtgag aacatgaata cgagcattct ctttaggttt gatcaccttt tgcaacgtgg
     4321 ataacacttc tgcggggttg agcggtttga gcaaataggc agcggcgccc aagcgcatgc
     4381 ctagcgcctt tttatccaca attgtaagca gaatgattgg aatattagtc gttgctgggt
     4441 cgtttttcag gtcgtgcaaa acttgccagc cgtctttctc tggcatgaga atatcgagca
     4501 aaatggcatc tggctttagc tcgcgggctt tttcctgccc gatgatgccg ttgcgagcgc
     4561 cgatgatctc gaattcttca ggtccgagat tttcgcgcaa aagataagta gcatcgggat
     4621 cgtcgtcgat gacgagaatg agctttttgg aagcatcagc tttggttgag gtgccagatc
     4681 gatggaaggg agccgtatcg ggattaagag acgaggcggg ttttgtccca tattgaatgg
     4741 ggagggtgag ggtgaatgtt gaaccttttc catgttcact ggtggctgtt agatcgccgc
     4801 ccaagaggcg tgccagattg cgactgatgg tgagacccaa cccggtgccg ccatattggc
     4861 gggtggtgct ggtatccgct tgctggaatt cttcgaagat tctgcctagg gcttcttcgg
     4921 tgataccaat acccgtatcg ctgacgttga cgaccaataa atcttcagcg cggtgtgcat
     4981 ctaatgtaat cttgcctgcc gcagtgaact tggcagcatt gctcaataag ttgagcacga
     5041 tctgtttgat tttatcttgg tcagaataga tcatgccgag atcttcttct acatctttta
     5101 ccaaacgaac cgtaggcttc atgagcggag ttgccgtatt ggcacacagg tccatcagcg
     5161 aactgacgtt gaagtttgcc gcctgcacgt ccatgcgccc ggcttcgatc ttggcaatat
     5221 cgagcacggt gttgatgagg ctaagcaaat gctcagaact gcttagaacc ttatcaagat
     5281 tttcggtttg tttatctggc aataagcctt cgctctttct tctcacaatg cgtgtgaagc
     5341 caatgatggc attgagcggc gtgcgtaact catggctcat attggcaagg aaggcgctct
     5401 ttgcctggtt ggcatgttct gctgcctgac gggcttcttg tgcttctttg aaaagacgct
     5461 gagtttcatt gaagaggcgg gcattttgaa tggcaacgcc catgttggca gagagagttt
     5521 gtagaatgcg caaattatct tcgttgaagg cgccggcttt atagctttga acatccacca
     5581 cgcctagtac ctgatcgccg accataatgg gcacacccat atacgacttg atttcttctt
     5641 catcttcagg ggcggtgaga taggctgccg caccatggtc attgatctct tgcgatgtat
     5701 tcaagagcaa aggctgtttc gagatgatga tcttggatgt aagcccttca cccaattccc
     5761 aaggtggttc atcttcgtag tggatattgt cgctaaagct gtatgccaat tgaacaatat
     5821 tcgtagcggc atcgtagagc agaatgtcta cgatctcggc gttgaagatc tcatggatct
     5881 tattgcctac gttgtgagtc acggtagtga cgtccagcgt gcgcgtcatc gattcactga
     5941 cactgttaag aatagaaagc tcggtggcgc gttgttcggt ctgattgagc aagtgttgag
     6001 tttgctcgaa tagctgggta ttttgaatgg caatgactgc ctgccgtgcc aggttgttca
     6061 tgaattccag ctcggcttcg gtgaagaggt tgtcggctcc gtgacgccag accgccatga
     6121 gaccttttag ctcgtcattg gcaagcaacg ggatggcaag catatgctcg tct
//
LOCUS       DAOQJE010000080         6162 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_7212_length_6162_cov_5.349599, whole genome shotgun sequence.
ACCESSION   DAOQJE010000080 DAOQJE010000000
VERSION     DAOQJE010000080.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 6162)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6162)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6162
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7212_length_6162_cov_5.349599"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..709)
                     /locus_tag="PLT08_03285"
     CDS             complement(<1..709)
                     /locus_tag="PLT08_03285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE03517.1"
                     /translation="MKSKSTWIISWAVIRLPLLGLLAYLFRYQLLSVFEKTVGFTGRM
                     DFFVFTISTFYARFLLYILFAIALAIGYLLIQKIKAPLTARYILWLVGIFCGVLFSFY
                     FIFQSTTSLIRTGFTVLLLAINTIPFEWFSNSSSRINPLLLVGVGISEAIFPQAYTSG
                     LLNLFPKLDQIKKWSWVSGVLIIPLLWVFLLIPYDNQRVFTLAEKVHADPAVEKFSTG
                     IYNWIELNPEKNLLYAVG"
     gene            complement(737..2113)
                     /locus_tag="PLT08_03290"
     CDS             complement(737..2113)
                     /locus_tag="PLT08_03290"
                     /inference="COORDINATES: protein motif:HMM:NF013584.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HNE03518.1"
                     /translation="MNPASLTLAEQRELIKSKEISASDLAAACYRQIDRLNPTLNAFI
                     TVIPQGEEETPDNRHIALLQGIPIAVKDLYNTKGIRTTGGSKFFADYVPSEDAFVVDK
                     IKKAGAHIIGKTNTHEIALGVTNNNPHFGACRNPWDIARTPGGSSGGSAVAVATGMAL
                     AAIGTDTGGSIRIPASLCGVVGLKPTYGRISLRGILPLSWNLDHAGPITKRVEDAAVM
                     LQVMGGYDPEDPASFKTLPGDFSSHMQDTIRDRKVALAVGTFVEEVTQPEVLASIRKA
                     ADVLAEQGAIIEEVNVDYLREGALANALMTQADGAAFHRERLKEHPDWFGADVRQRLE
                     TGAGFTSSEYALARRTQAEVRRRCDLLFEEYDVLILPTTPIVAPVLEGENAVERARLL
                     TRLTSPFNITGLPALSVPCGFSSEGLPIGLQIASRAWNESGVLRIGYAYQQATEWHKT
                     KPSIVATA"
     gene            complement(2222..>3736)
                     /locus_tag="PLT08_03295"
     CDS             complement(2222..>3736)
                     /locus_tag="PLT08_03295"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004329 - formate-tetrahydrofolate ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HNE03519.1"
                     /translation="IPMEDFNLHLTGDIHAITASHNLVAAALDARVMHEKEQDDEKLF
                     NALCPPNKKGQRKFSPTMLRRLEKLGIKKTNPDDLTPEERSRFARLDIDERTITWRRV
                     VDINDRMLREVEVGRGKDEVGHAHMSGYDITVASEIMAVLALTTGIEDMRERFGKMVV
                     ATSKRGDAVTVEDLGVAGAVTVLMKDAIKPNLMQTLEGTPATVHAGPFANIAHGQSSI
                     IADKIALKLVGKDGFVVTESGFGADIGMEKFFNIKCRYSGLIPHVVVMVATVRALKMH
                     GGGPKVVAGKPLAPEYTDENLELLKAGLVNLERHIQNAMKFGVKVIVAINSFATDTEA
                     EVELIRKSAIEFGAMDAIKCTHWADGGKGTKKLAEAVVKAAEQPSNFKFLYDPKLPIK
                     EKIEIIAKEIYRADGVDYSPEAEEQVERYTRLGFNDLPICMAKTHLSFTTDATKKGAP
                     TGFRITVREVRASVGAGFLYPILGDMRTMPGLPTRPSFYDVDLDMETGKVLGLF"
     assembly_gap    3737..3836
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<3837..4096)
                     /locus_tag="PLT08_03300"
     CDS             complement(<3837..4096)
                     /locus_tag="PLT08_03300"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0004329 - formate-tetrahydrofolate ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HNE03520.1"
                     /translation="MATKGKSVKKGAKAAPKKVAKKVVAKKKAAPKPSNTFQPTKIKL
                     LRPVPSDIDIAQAGKLKPITQIAKELGIRDDELELFGPYKAK"
     gene            complement(4192..4992)
                     /locus_tag="PLT08_03305"
     CDS             complement(4192..4992)
                     /locus_tag="PLT08_03305"
                     /EC_number="2.7.4.26"
                     /inference="COORDINATES: protein motif:HMM:NF040647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isopentenyl phosphate kinase"
                     /protein_id="HNE03521.1"
                     /translation="MTNELVLLKLGGSLITDKDTPYTPRLDKLANLAKEIKTALDSTP
                     GLKLILGHGSGSFGHVAAKKHGTRDGVQTKEQWLGFTEVRLQAAELNRYVVTSLFEAG
                     IPALPFPPSASMVSDKRKVTHHNVETIRRALGVDLLPVVQGDVAFDETLGGTILSTED
                     VFTFLVDQFPTTRILLAGIEAGVWADFPARTKLVKQIQLSDYEAMRSGIKGSVSTDVT
                     GGMKAKVEEMLSLIQKKPGLTVQIFSAEEPGFLTRALQGENVGTLLTA"
     gene            5059..5904
                     /locus_tag="PLT08_03310"
     CDS             5059..5904
                     /locus_tag="PLT08_03310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE03522.1"
                     /translation="MDIRTGVFVLALFAAVGAFLSFRGAIRNMQAARKISFYSLRKRY
                     NNTAWRLVFLAFILIGVAFWFPSGGAQVMYQVFPPSVTPSLTPTITLTPTITLTPTIT
                     LTPTLTPTPLVSDTPTLTPTPFLPAAIEALFAGPVTPNPDSIFTAIQFSTEFDGVNAV
                     DPKTVFTLPIDTIYGGFDYNNTQPGVQWTALWFRNGELVCYETEPWREEWGTGGIGGY
                     TSCSSPLDGWQAGQYEVQIFMGYDWKVVGRFVVMDSLTPSVTVTGTPDLLQTGTPTIA
                     PTATP"
     gene            5927..6127
                     /locus_tag="PLT08_03315"
     CDS             5927..6127
                     /locus_tag="PLT08_03315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE03523.1"
                     /translation="MTEKAFQEYYPEHLSHCYGCGTMNELGHQIKSYWDGEESVCHFQ
                     PKPYHTAIPGYVYGGLLASLID"
BASE COUNT         1519 a         1458 c         1477 g         1608 t
ORIGIN      
        1 gtccaactgc gtataacaga tttttttctg gatttaattc gatccagtta tagatccctg
       61 tagaaaattt ttcgacggca gggtctgcat gaaccttttc tgcaagcgta aagacgcgtt
      121 gattgtcgta cgggataagc aaaaagaccc aaagtagcgg aatgatcaaa accccgctaa
      181 cccaagacca cttctttatc tggtcgagtt tcggaaataa atttagtaat ccggaagtat
      241 aggcttgcgg gaaaatagct tcgcttatac caacaccaac aagaagtaac gggttgattc
      301 gacttgaact attcgaaaac cactcaaaag gaatggtatt gattgcgagc aataaaactg
      361 taaaaccggt acgtattaat gaggtagtcg attgaaaaat gaaataaaag ctaaacaata
      421 ccccgcagaa tatgcccaca agccacaaga tatatcgtgc agtaagtgga gctttgattt
      481 tttgaatcag tagatagcca attgccaaag ctattgcaaa gagaatatac agtagaaacc
      541 gcgcataaaa tgtgctgatt gtaaatacaa agaaatccat ccttccggta aacccgactg
      601 ttttttcaaa aacggaaagg agttggtatc taaaaagata tgccaataaa cctaaaagag
      661 gcagtcgtat aactgcccaa gaaatgatcc atgtgctttt agacttcata ttcttatgat
      721 aacaggggat gaaattttac gcagtagcaa cgatagatgg ctttgtttta tgccactctg
      781 tcgcctgttg gtaggcgtat ccaatccgta aaacgccaga ctcattccat gcgcgagagg
      841 caatctgtaa cccaatgggc aatccttctg aagaaaagcc gcatggaacg gataaagctg
      901 ggagcccagt gatattgaat ggcgaagtca ggcgggttaa caaacgagcg cgctcaacgg
      961 cattttcacc ttcaagaact ggagcaacaa tgggggttgt cggaagaatc agaacatcat
     1021 attcttcgaa gagaagatcg catcgcctgc ggacctctgc ttgagtgcgt cttgcaagcg
     1081 catactcact ggatgtgaat cctgctcccg tttccaaccg ctggcggacg tctgctccaa
     1141 accaatcagg gtgttccttg agccgttcac gatggaaggc agcgccatcc gcttgggtca
     1201 tcaacgcatt cgcaagtgcc ccttcacgta agtaatctac atttacttct tcaatgattg
     1261 cgccttgttc ggcaagcaca tcggctgctt tacggataga ggctaaaact tcgggctgag
     1321 tcacttcttc cacaaaggtc ccaacagcga gcgcaacttt acggtcgcgg atcgtatctt
     1381 gcatatggct tgaaaaatca ccgggcaatg ttttgaaaga agctgggtct tctgggtcgt
     1441 atccgcccat gacttgcagc atcacagccg catcttctac tcgtttggtg atgggacccg
     1501 catgatccaa attccatgaa agcggtagaa tgcctctcaa actgatgcgt ccataagtgg
     1561 gtttgagtcc tactacacca cataatgaag ctggaatgcg aatggaaccg cctgtatcag
     1621 tgcctatagc agcaagtgcc atgcctgttg ccactgcaac tgcgctcccg cctgatgagc
     1681 cgcccggggt gcgggcaata tcccaagggt tgcggcacgc cccaaaatgc ggattgttat
     1741 tggttacacc aagagcgatc tcatgggtat tggttttgcc aataatatgc gctcctgctt
     1801 ttttgatctt atccacaacg aatgcatctt cactaggcac atagtctgcg aagaatttag
     1861 agccacccgt tgtgcggatg cctttggtat tgtataaatc tttaactgca atcggaatgc
     1921 cctgtaaaag agcgatgtgt cggttatcag gcgtttcttc ttcaccttgt ggaatgacag
     1981 tgatgaaggc attaagcgtc gggttgagac ggtcaatttg acggtaacaa gctgcggcga
     2041 ggtcagaagc gctgatttcc ttgcttttga tcaattctct ttgttcagct aaagtcaggg
     2101 aagcgggatt cataacggtg aattatattc aatttatact tattttcaat atagcctatc
     2161 ggtactgaaa aaacaggtca cgcctaacgc gtgacctgca tttttgtagg tagagattgt
     2221 tctagaacaa cccgaggacc ttgcctgttt ccatatccaa gtccacatcg tagaaagatg
     2281 ggcgagtggg caaaccgggc atggtgcgca tgtcgccgag gattgggtac aagaagcctg
     2341 cgccaacgct ggcacgcact tcgcggacgg tgatacggaa gcccgtaggc gcacctttct
     2401 tagtggcatc agtggtaaag gagagatggg tctttgccat gcaaatcgga aggtcgttga
     2461 acccgaggcg agtatagcgt tcgacctgtt cttcggcttc aggcgagtaa tccacaccat
     2521 cggcacgata gatttccttg gcgatgatct cgattttttc cttgatggga agcttcggat
     2581 catacaggaa cttgaagttg ctcggctgtt ctgcagcttt aaccaccgcc tcagccaatt
     2641 tcttagtgcc cttaccgcca tcagcccagt gcgtacattt gatggcatcc atggcgccga
     2701 actcaatggc agatttgcgg atgagttcaa cctcagcctc tgtatctgtc gcaaaggagt
     2761 tgatagcaac aataactttg acaccgaatt tcattgcatt ctgaatatgg cgttcaaggt
     2821 taacgagacc cgccttgagc aattccagat tttcgtcggt gtattcaggg gcgagcggtt
     2881 tacccgccac cacttttgga ccaccaccat gcatcttcaa tgcgcggacg gttgcaacca
     2941 tcaccactac atgcgggatc aagccagagt aacggcattt gatattgaag aacttttcca
     3001 taccgatatc ggcaccgaaa ccagattcag tgaccacgaa accgtctttg ccgactaact
     3061 tgagcgcgat tttgtcagcg atgatggaag actgtccgtg agcgatattg gcgaaaggtc
     3121 cggcgtgaac cgtggcggga gtcccttcga gggtttgcat tagattcggc ttgatggcat
     3181 ccttcatcaa gacagtgact gcaccagcca caccgagatc ttccacagtg acagcgtcgc
     3241 cgcgtttgct ggtggcaact accatcttgc cgaagcgttc gcgcatatct tcgataccag
     3301 tagtcaatgc gaggaccgcc atgatttctg aggcaacggt aatgtcatag ccagacatat
     3361 gcgcatgacc cacttcgtct ttaccacgac ctacttccac ttcacgcagc atgcggtcat
     3421 tgatatctac cacacggcgc caggtgatgg tgcgttcgtc aatgtcgagg cgggcgaagc
     3481 gagagcgttc ctcgggggtg agatcgtctg gattggtttt cttgatgcca agtttttcca
     3541 aacggcgcaa catggtgggt gagaatttgc gctgtccctt tttgttgggc gggcaaagcg
     3601 cgttgaagag cttctcatca tcttgttctt tttcatgcat cacacgcgca tcgagagcgg
     3661 cggcaaccaa gttatgtgag gcggtaatgg catgaatatc gccggtgaga tggagattga
     3721 aatcttccat cggaatnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3781 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnatct
     3841 tggctttgta aggtccgaac aattcaagtt cgtcatcgcg aataccaagc tctttggcaa
     3901 tttgggtgat cggcttaagc tttccagctt gtgcaatatc aatatccgaa ggcacaggtc
     3961 gaaggagctt tatcttagtc ggttggaatg tgttgctggg cttgggagca gctttcttct
     4021 ttgctacgac cttttttgca actttcttag gcgcagcttt tgcacccttc ttaacggact
     4081 ttcctttggt tgccatagag tctccttgaa aattatatat aatttattct atccccaatt
     4141 atcagacaaa tttaaaaaat tgcaagagca aaccgtcatt tttttgtcat ttcatgcagt
     4201 tagcaacgtt cccacatttt caccttgcaa ggcacgtgtc aagaaacctg gctcttctgc
     4261 cgagaaaatt tgtacagtca aacccggctt cttttggatg agggacagca tttcttccac
     4321 tttggctttc atcccaccag tgacatcggt gctcacggaa cccttgatgc cagaccgcat
     4381 cgcttcataa tccgacagtt gaatctgctt tacgagcttt gtcctcgctg ggaaatctgc
     4441 ccacactccg gcttcaatcc cagccagcag aattcgagtg gtagggaatt ggtccacgag
     4501 aaaggtgaaa acatcttcgg tagaaagaat ggtgccgcct agggtttcat caaaagcgac
     4561 atctccctgc accacaggta gcaaatccac gcccaaggcg cggcggatgg tctctacatt
     4621 gtgatgggtc actttccgtt tgtctgaaac catcgaagca gatggcggga aaggtagcgc
     4681 aggtatccct gcctcaaaga gagaggtgac aacataacga tttaactcgg cggcttgaag
     4741 ccgaacttca gtgaagccca gccattgttc cttcgtttgg acgccatcgc gagtgccatg
     4801 cttctttgcg gcaacgtgcc cgaacgagcc agaaccatgc ccgagaatca gtttcaagcc
     4861 cggggttgag tccagagccg ttttgatttc cttggcaagg tttgccagtt tatccagcct
     4921 tggggtgtag ggagtgtctt tatcggtaat gagcgatccg cccagtttga ggagaacgag
     4981 ttcgttggtc atagtgcttg ttttaacaca gatttttgaa attgtcgcgc actttgcttt
     5041 actgggtata attttggcat ggatattcgt actggtgtct tcgtcctcgc cctgtttgcg
     5101 gctgtggggg cgtttctttc tttccgtgga gcgatccgta atatgcaggc ggctcgcaag
     5161 atttcttttt acagcttacg taagcgttac aacaataccg cttggcgttt ggtttttctt
     5221 gcctttattt tgatcggtgt tgctttttgg ttcccgtctg gtggggcgca agtgatgtat
     5281 caggtctttc cgccttctgt caccccatcg ttgacgccga cgattacact gacgccgacc
     5341 attacgttaa cgccgaccat caccttaacg ccaacactaa cgcccacgcc attggtctct
     5401 gatacaccaa cattgactcc aactccgttc ttgccggctg cgattgaagc gctttttgct
     5461 ggacctgtca cgccgaaccc tgattcgata tttaccgcca ttcaattctc tacagagttt
     5521 gacggcgtaa atgcagtgga cccaaagact gtttttacac ttcctattga cacgatttat
     5581 ggcggtttcg attacaacaa cacgcaaccc ggcgtgcagt ggaccgcgct ctggtttcgc
     5641 aatggcgaat tggtttgtta tgagaccgaa ccttggcgtg aagaatgggg cacgggcggt
     5701 attggtgggt atacctcttg ttcttcacct ttggacggtt ggcaggcagg gcaatatgaa
     5761 gtgcagatct tcatgggata cgattggaaa gtcgttgggc gttttgtggt gatggatagc
     5821 ctgacccctt ctgtgaccgt aacagggacg ccagatttac ttcaaacagg aaccccaacc
     5881 atcgcgccca cggcaacacc gtaaatctat aacaacagga atacacatga cagaaaaagc
     5941 ctttcaagaa tactatcctg aacatttatc gcattgctac ggttgcggca cgatgaacga
     6001 attagggcat cagatcaaaa gctattggga cggggaagag tcagtgtgcc actttcagcc
     6061 taaaccttac cataccgcca ttcctggtta tgtgtatggt gggttgctcg cttctctcat
     6121 tgactgaagc gagcaaccca ccatacacat aaccaggaat gg
//
LOCUS       DAOQJE010000081         6145 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_7232_length_6145_cov_7.210837, whole genome shotgun sequence.
ACCESSION   DAOQJE010000081 DAOQJE010000000
VERSION     DAOQJE010000081.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 6145)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6145)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6145
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7232_length_6145_cov_7.210837"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..107)
                     /locus_tag="PLT08_03320"
     CDS             complement(<1..107)
                     /locus_tag="PLT08_03320"
                     /inference="COORDINATES: protein motif:HMM:NF014036.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosome-binding GTPase"
                     /protein_id="HNE03524.1"
                     /translation="MKLGIIGLPQSGKTTIFNALTRGNAPTNASAGRIEV"
     gene            174..256
                     /locus_tag="PLT08_03325"
     tRNA            174..256
                     /locus_tag="PLT08_03325"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:208..210,aa:Leu,seq:caa)
     gene            618..1868
                     /locus_tag="PLT08_03330"
     CDS             618..1868
                     /locus_tag="PLT08_03330"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008878 - glucose-1-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase"
                     /protein_id="HNE03525.1"
                     /translation="MPSYNEVLAVILGGGRGARLYPLTQMRSKPAVPIAGKYRLIDIP
                     ISNCINSEIFRIAILTQFNSVSLHRHITRTYNFDSFHQGWVQIWAAEQTIESADWYQG
                     TADAVRKQMLEIQATGAKYVLILAGDHLYRMDYKAMAEFHWANNADITVAVQPVAKDE
                     ANRFGVLKRESDGTISDFYEKPKDPDVQKRFVSRDDPERPFLGSMGIYMFNTKVLLDF
                     ISNFPDYDDFGGDIIPQAIKTHKVFGFDFDGYWQDIGTIRSFYETNLMLTKSNTPFDF
                     YDAKLPIYTDTRYLPSSIVEDSQMKDVLIAEGSRVLKSEITHSIIGIRSQIAGGCVIK
                     DSIVMGSDYYEREHRAGTPLGIGPNCHIEGAILDKNVHIGENVTIRPFPRNTDIDSEK
                     WYVRDGIVIIPKDVEIPSGTVIAP"
     gene            1992..2237
                     /locus_tag="PLT08_03335"
     CDS             1992..2237
                     /locus_tag="PLT08_03335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE03526.1"
                     /translation="MKSTREFWQRWAESLRRYQLHDLVASFLEAGSPLALLGAQAIYF
                     GGGLVKNDQLTALAGLLEDENETRAFVAFLSHNGASS"
     gene            2234..2965
                     /locus_tag="PLT08_03340"
     CDS             2234..2965
                     /locus_tag="PLT08_03340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE03527.1"
                     /translation="MTALAILGVAAFLLLSLTLWKRRSPSYLREIPAFTKLAHSLGLS
                     IEDGKRLHISLGHGGLLDAHGGSAFAGLALLRNIAERTSVSDMPAVASAGDPTLGLLS
                     QDTLQAGYQAAGVEDAYVHTTGRVTGLSPFSYAAGAMQIPQNENVSTNIVIGHLGAEA
                     GLITDAAERNNIKLIGSSDNLVGQSILFANTQDPLIGEELFAAGAYLNEDPAHAASLT
                     VQDIFRWLIILILLGGAALKFLGTL"
     gene            2965..3579
                     /locus_tag="PLT08_03345"
     CDS             2965..3579
                     /locus_tag="PLT08_03345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE03528.1"
                     /translation="MRIITAIIAIVVGILVLLGYFLPQAAIFQVILLDWAIILAGAAT
                     LAGVLNLVTVHSEKIRTRSKDNIYSAILILGLFATFVFSLLLKPQNIYVQTFILNGII
                     VPIEASLMALLAVSLLYAAVRLLRHRADLMGIVFVLTAALLFFGSATLPFGDVPIMST
                     LVRPWVSQILALGGARGILIGVALGTLTTGLRVLFGIDRPYGGK"
     gene            3579..>6145
                     /locus_tag="PLT08_03350"
     CDS             3579..>6145
                     /locus_tag="PLT08_03350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE03529.1"
                     /translation="MAEMITCPVCGTKNPANQAVCQRCQTPLNNALFQPGQSPIHKDT
                     GELEPLLPQWLKDARQSAKEEGETQQQTQQPPKPEAPAKPVASSTDFLAGLQSQAGDD
                     DDEEIPDWLASITGTPAPKQKPNEVVEEPSGTRWVETGNKSDFQQAEPEAEEEVPSWL
                     TGMQQQPNPDEKDELTDWFRSSSSEAPSAQPLSFEQPQQSPTFDSAFTPSASNDTPDW
                     LKQMAANAGEKEPESASDSSFGSSDWFSQPAESNPAPQADPFAQSSDTPDWLHQMQEN
                     LDSSAQQQPSASPFAASSGDSDLPDWLNQMQAGAVPAEPAQPAEPFNLSSDTPDWLNQ
                     MRSDSTPAESAPSAEPFSFSSDTPDWLSQLPADNAASQQPAADPFNASSSADMPDWLS
                     GLGSVPGGENSPFVESTPAESEPFSSNSDLPSWLAPEQDAAKKTDSKPRWLQSDQEVA
                     SGEPAWLASNDKTLVPESEQAADETPVNDVFSDMPDWLKSAAPASTIFDDAPAFETPK
                     AETPKQQPSSPFESAPAFSEDAFPAQNDALFTEMPDWLSNAMEEPASGGMIPEPIKGG
                     DALAPDILPSWVEAMRPSDQGMSNIVNAANDATLESRGALAGLSGVLPLGAGFAPTSK
                     PKAYSIKLNASDEQVKHAGILEQILAAETSAVSLTSEKSLGSSRVLRWSIAFVLFIVT
                     ILTSFLGTQIFSTPQAQPNELKLAVAVSQAIPDSAPVLVAVDYEASRAGEIEAAAAPL
                     FDNMLLLKHPRLTFVSSNESGAILAERLMNGPLAFHVQNGVSYLNLGYLSGGQMGIRA
                     FAQNPTSVAPSDVNGQPAWASPVLQNVTALNQFALIILLTDDADAARAWVEQTEDSRG
                     "
BASE COUNT         1507 a         1531 c         1455 g         1652 t
ORIGIN      
        1 acttcgatgc gtccagcaga tgcgttggtg ggggcgttgc cacgggtgag ggcgttgaaa
       61 atcgtcgttt ttccagattg ggggagtcca ataattccta gcttcatctt gaccttatca
      121 atgggagtgg tatacttgcg ttcgcctcgc taatgcgaga ccacaacaat taagccgaag
      181 tggcggaata ggcagacgcg cacgactcaa aatcgtgttc cctccgggaa tgagggttcg
      241 attccctcct tcggcacacg gattataccc tacgggtact tgaccctaag agcaaataat
      301 ccacctctgg cagcgtcctc cgcaatggag gacgtttgat ttttatatgt accctactct
      361 gtaaggttga ccaggtcatt tggggattgt agaatgaaaa tagaaacaag tatgggtttt
      421 atacgtattt atattaagct gtctagaccc gtaggaggag tccctcatga aactttctct
      481 ctttttcatg ttgtttgatg ttttcgtgat tttggcatac ccaattgctt atgtaattca
      541 tcgcattcgt aaggtgaagg gtgtaaaata gggagcattg ctcgacattc ctgaacgaaa
      601 attccaggag gctccatatg ccgtcgtaca acgaagtcct ggctgtgatc cttgggggtg
      661 gacgcggggc acgcctttac ccgctcacac aaatgagatc taagcctgcc gtcccgattg
      721 cgggaaaata ccgtctcatt gacattccca ttagtaattg tattaactca gagatatttc
      781 gtattgcgat tctgacccag ttcaactcgg tctcattgca tcgtcatatc acccgcacct
      841 ataactttga ttctttccat cagggttggg tgcagatctg ggcagccgaa cagacgatag
      901 aaagtgccga ctggtatcaa ggcacagccg atgctgttcg caagcagatg cttgaaattc
      961 aagccacggg tgcaaagtat gtactcatcc ttgcagggga tcatctgtac cgtatggact
     1021 acaaggcaat ggctgagttt cactgggcga ataatgcaga tatcaccgtc gccgtgcaac
     1081 ccgttgcgaa ggacgaagca aatcgctttg gcgtcttgaa gcgtgaatca gacggtacga
     1141 tctcagattt ttacgaaaag cctaaagatc cagatgtcca aaaaagattt gtcagccgtg
     1201 acgaccctga acgacctttt ctcggttcca tgggtatcta tatgttcaac accaaagtac
     1261 tgttggactt tatcagcaac ttcccagatt acgatgactt tggcggcgat attattccgc
     1321 aagcgattaa gacccataaa gtctttggct ttgattttga cggctactgg caagatattg
     1381 gaacgatccg ctcgttctat gagaccaatt tgatgctgac gaaatcaaat actccatttg
     1441 atttctatga cgcaaaattg ccgatctata ccgatacgcg ttatctgcct tcttccattg
     1501 tggaagatag ccagatgaaa gatgtattga tcgctgaagg tagccgggtc ttgaaatctg
     1561 agatcacgca ttcgatcatt ggtattcgca gtcagatcgc tggtggctgt gtgatcaaag
     1621 acagcattgt gatgggttct gattactatg aacgtgaaca ccgtgcaggc acacccttag
     1681 gaattggacc aaattgccac attgaaggtg ccattctcga taagaatgta cacattggcg
     1741 agaatgtaac cattcgcccc ttcccacgca ataccgatat cgacagcgaa aaatggtatg
     1801 tgcgagatgg tattgtcatc attccaaaag atgtagagat accatcgggt acggtcatcg
     1861 ctccataaac aatgaacccc tgaagatcga attcttcagg ggttttttct tgaaatattc
     1921 acaaaactgt aacatttagc gctcttatgc agcaggggca accttaaaat tatgctatac
     1981 tcgcattaac tatgaagtca acgcgcgaat tctggcaacg ctgggctgag tccttgcgcc
     2041 gttatcaact tcacgatctt gtcgcctcat ttctagaagc aggaagcccg cttgccctgc
     2101 tcggtgctca ggcgatttat tttggtgggg gacttgtcaa gaatgaccag cttacagctc
     2161 ttgccggttt gcttgaagat gaaaacgaga cacgagcctt tgtcgctttt ttgagtcaca
     2221 atggagcgag ttcatgaccg cactagctat tcttggcgtc gcagctttct tattacttag
     2281 ccttacatta tggaagcgac gctctccctc atatttacgt gaaattcccg cttttacaaa
     2341 gcttgctcat tctcttggct taagcatcga agacggaaaa cgccttcaca tttcgcttgg
     2401 gcatggcggc ctgctcgatg cacacggcgg atctgccttc gccgggcttg ctctactgcg
     2461 gaatattgct gagcgcacct ctgtgagtga tatgcctgct gtcgcctcgg caggggaccc
     2521 gacacttggt ctgttatccc aagacacctt gcaagccgga taccaagccg ccggcgtcga
     2581 agatgcctac gtccacacca ccgggcgtgt tacgggttta tcaccattta gttatgcggc
     2641 tggtgcaatg caaattcctc aaaacgagaa tgtttctacg aacatagtca ttggtcattt
     2701 gggagcagaa gctgggctta ttacagatgc tgccgaacgc aacaacatca aactgattgg
     2761 ctcgtctgat aatctggtgg ggcagtccat tctttttgcc aatacccaag atccactgat
     2821 cggtgaagaa ctctttgctg caggcgccta tctgaatgaa gaccctgccc atgctgccag
     2881 cctcactgtc caagatattt tccgctggct gatcatcctc atccttttgg gtggggcagc
     2941 acttaaattc ttagggacgc tctaatgcgc attatcactg ccattatcgc cattgttgtc
     3001 ggtatcctcg tcttgctggg atatttcctg ccgcaagccg cgatctttca agtcatcttg
     3061 ctcgattggg caattatcct ggcaggggca gcaaccttgg cgggtgtcct taatctcgtt
     3121 acagttcact cagaaaagat tcgcactcgt tcaaaagaca atatctatag tgctattttg
     3181 atcttgggtt tgtttgctac atttgtattt tctttgttgc tcaagccgca aaatatttat
     3241 gtgcaaacct ttattttgaa tggcatcatt gttccgatag aagcctctct tatggcgttg
     3301 ctggcagttt cattgttata tgctgctgtc cgcttattac gccaccgagc agacttaatg
     3361 ggtattgttt ttgttctaac agcagctttg cttttctttg gttctgccac attgcctttc
     3421 ggtgatgttc ccattatgag caccttggta cgcccctggg tctctcagat tttggcattg
     3481 ggtggtgcgc gcggtatttt gatcggtgta gcacttggca cacttacaac aggtctgcgt
     3541 gtcttgtttg gcatagaccg tccatacggg ggtaagtaat ggctgaaatg attacctgcc
     3601 ctgtctgtgg tacgaaaaac cctgccaacc aagcagtttg tcaacgctgt caaacgccgc
     3661 ttaataatgc tttattccaa ccggggcaat ctccaatcca caaagatact ggtgagttag
     3721 agccacttct cccgcaatgg ttgaaagatg cgagacaatc agctaaagaa gaaggcgaga
     3781 ctcaacagca gactcaacaa ccgcccaagc cagaagcgcc tgccaaacca gtcgcctcaa
     3841 gtaccgactt tttagcaggt ctgcaatctc aagcagggga cgacgacgat gaagagatcc
     3901 ccgattggct tgctagcatt acaggtacgc cagcacccaa acaaaagccc aatgaagtag
     3961 tagaagaacc tagtggaact cgctgggtgg aaactggcaa taaaagtgat ttccagcaag
     4021 ctgaaccaga ggcggaagaa gaagtgccaa gctggttgac aggcatgcag caacagccca
     4081 accctgatga aaaagacgaa ctgaccgatt ggttccgttc atcgtcttca gaggcgccaa
     4141 gtgctcagcc attgtctttt gaacagccac aacagagccc aacatttgat tctgctttta
     4201 ctccttctgc atcaaacgac acgccagatt ggctaaaaca aatggctgcg aatgccggag
     4261 aaaaagaacc ggaatcagct tccgattctt cgtttggatc ttcagactgg ttcagccaac
     4321 cagcagaatc aaaccccgct cctcaagccg acccatttgc ccaatcttcg gatacgcccg
     4381 attggttaca ccaaatgcaa gagaatctcg attcttcggc acaacagcaa ccttcggctt
     4441 caccgtttgc agcttcatct ggtgattcag atttaccgga ttggcttaat caaatgcaag
     4501 caggcgctgt tcctgcggaa cctgctcaac ccgctgagcc gtttaatctc tcttcagata
     4561 cgcctgattg gttgaatcag atgcggtctg attcaacacc tgccgaatca gctccatcag
     4621 cagagccatt tagcttctct tcggatacgc ctgattggct cagtcaactt ccggcagata
     4681 atgcggcatc tcagcaacct gctgcagacc cgttcaatgc ttcatcttct gcggatatgc
     4741 ccgattggct aagtggattg ggatctgtgc ccggcggaga aaactctccg tttgttgaat
     4801 caactccggc tgagtctgag ccgttctcgt cgaatagtga cttaccatct tggttggctc
     4861 ccgagcagga tgcagccaag aaaacggatt ccaaaccgcg ttggttgcaa agtgaccagg
     4921 aagttgcttc aggggagccc gcttggttgg catctaatga caagactctt gttcctgaat
     4981 ctgaacaagc tgcagatgaa accccggtca atgatgtctt tagcgatatg ccagattggt
     5041 tgaaatcggc tgcgcctgcg tcgacaattt ttgatgatgc tcctgccttt gaaaccccga
     5101 aggcagagac ccctaaacag caaccttctt ctccatttga atctgcacct gcattttcag
     5161 aagatgcttt ccctgcacaa aatgatgcgc tctttaccga aatgcctgat tggctttcga
     5221 atgcaatgga agaacccgct tcaggcggga tgatccctga acccatcaaa ggtggcgatg
     5281 cgcttgcacc agatattctg ccttcgtggg tggaagcgat gcgaccttct gaccagggaa
     5341 tgtctaacat tgtgaatgcc gcaaacgatg ccacacttga atcacgcggc gcattggcgg
     5401 ggctttctgg tgtgttgcct ttgggggcag gctttgcgcc aaccagcaaa ccaaaagctt
     5461 attccatcaa attgaatgcg agtgatgaac aggttaagca tgcaggtatc ctggaacaga
     5521 tccttgcggc agaaacatct gcggtttcgc tcacttctga aaaatctcta ggttcctcac
     5581 gcgtgttgcg ctggtcaatt gcttttgtgc tattcattgt caccattctg accagtttcc
     5641 tcggtacgca aatcttctcc acacctcaag cgcagcccaa tgaattaaag ttggcggtgg
     5701 cagtttctca agccatccca gatagtgcgc ctgtattggt cgctgtcgat tatgaagctt
     5761 ctcgtgcggg tgaaattgaa gctgctgcgg cacctttgtt tgacaatatg ctattgttga
     5821 aacatccgcg cctgaccttt gtttcaagca atgagtcggg ggcgatcctt gccgaacgcc
     5881 tcatgaacgg accgttagct tttcacgtcc aaaatggcgt ttcctacctg aatctaggat
     5941 atctctctgg cggacagatg ggaatccgtg catttgcgca gaaccccacc tctgttgcgc
     6001 cgtctgatgt caatggtcaa cccgcatggg catcgcctgt tttacagaat gtaaccgccc
     6061 taaatcaatt cgctctcatc atcttactga ccgatgatgc tgatgctgca cgtgcctggg
     6121 tggaacaaac cgaagactca cgtgg
//
LOCUS       DAOQJE010000082         6129 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_7263_length_6129_cov_6.181923, whole genome shotgun sequence.
ACCESSION   DAOQJE010000082 DAOQJE010000000
VERSION     DAOQJE010000082.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 6129)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6129)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6129
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7263_length_6129_cov_6.181923"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..293)
                     /locus_tag="PLT08_03355"
     CDS             complement(<1..293)
                     /locus_tag="PLT08_03355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE03530.1"
                     /translation="MQTTKRPFALALFASLISTLLFSCSEQENTATLSVEITQTAEPT
                     VLPSPTSTPLVAANENPLTGLPVKDASLLDLPALLVSISHFPVEARPQAGLSFA"
     gene            complement(352..837)
                     /locus_tag="PLT08_03360"
     CDS             complement(352..837)
                     /locus_tag="PLT08_03360"
                     /inference="COORDINATES: protein motif:HMM:NF023395.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIT domain-containing protein"
                     /protein_id="HNE03531.1"
                     /translation="MKHLWSPWRMNYIEGNKKEEGCVFCNAQSKPDSAENLIAFRGKS
                     AYVILNRFPYTSGHLMVIPFSHVATIEELVSETRAEMMELTSRCTTELRQIYKPHGFN
                     VGINMGEAAGAGVLGHVHIHIVPRWAGDTNFMSSVGETRVLPESLEQTYQRVHSAFNS
                     S"
     regulatory      923..1090
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00380"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="The M-box (ykoK) riboswitch appears to function as
                     a genetic 'off' switch that is important for maintaining
                     magnesium homeostasis in bacteria; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="magnesium"
                     /db_xref="RFAM:RF00380"
     gene            1432..2811
                     /gene="mgtE"
                     /locus_tag="PLT08_03365"
     CDS             1432..2811
                     /gene="mgtE"
                     /locus_tag="PLT08_03365"
                     /inference="COORDINATES: protein motif:HMM:TIGR00400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HNE03532.1"
                     /translation="MNPTLAMINLEALIEARDFNTLREQTKNWSAGDLAELMEPLSAE
                     KEAVIFRLLPREQAAGVFSYLSEERQEELLKAMAREDVANILNAMSPDDRTSLLEEMP
                     ANVIQHLLGLLSAEERTVASQLLGYEEDSVGRLMTPDFVRVRPQWTIAQALEHIRRYG
                     KDSETMSMIYVIDDNNKLVDDLRIRQILLASPTDTVADLMDSRFVYLKTTDNRETAVE
                     AFKDSDLNALPVTDKEDTLIGIVTVDDILDVAEEEATEDIQKMGGSEALDEPYMKIAI
                     SKMVQKRATWLIILFISEMLTATAMGNFEHEIAKAVVLSIFVPLVISSGGNSGSQAST
                     LIIRAMALGEVRLVDWWRVMRREFLSGLSLGAILGVIGFIRISVWHYLFDAYGPHWFL
                     LAVTVGIALVGIVLWGSLSGSMLPFLLKRVGLDPATSSAPFVATLVDVSGLIIYFTVA
                     AVILKGTLL"
     gene            2908..4110
                     /gene="xseA"
                     /locus_tag="PLT08_03370"
     CDS             2908..4110
                     /gene="xseA"
                     /locus_tag="PLT08_03370"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HNE03533.1"
                     /translation="MQPDLFSATLTVSQLTLRIRRLLEDNPELQDVWVEGEISNLSRP
                     ASGHIYFTLKDKNASLRCVMWKTDALRTRPSMQEGGAVEVHGKIAVYEPQGQYQLIAN
                     LIRPKGEGALFQEFLRLKSMLEAEGLFDSDRKREIPAFPKTIGIVTSATGAALRDMLN
                     TIRRRQPLARVILAPSPVQGLDAPPALVRAIQDINRQNPDVILVARGGGSIEDLWAFN
                     DERVVREVANSNAPVISGVGHETDFTLTDFASDLRAPTPTAAAELATSTTLDDLHLQM
                     TSYQTRLTDLISGLLADHQASVSALAARLKYVSPERRIQAEYQHIDELSRRAVSALTH
                     RIQLQSARVDGISKRLGALNPAGILSRGYAIITREADGKVVSQVLQAQGDMTVRVSDG
                     EFTVKRNS"
     gene            4146..4391
                     /gene="xseB"
                     /locus_tag="PLT08_03375"
     CDS             4146..4391
                     /gene="xseB"
                     /locus_tag="PLT08_03375"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HNE03534.1"
                     /translation="MAKAKPTQKPVEELTYEEALAELEQTVELLEGEQNPLDDAMKLF
                     ERGQALAARCGVLLEAAQLKVQKLVGETTLDFEEEDE"
     gene            4388..4840
                     /locus_tag="PLT08_03380"
     CDS             4388..4840
                     /locus_tag="PLT08_03380"
                     /inference="COORDINATES: protein motif:HMM:NF014711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divergent PAP2 family protein"
                     /protein_id="HNE03535.1"
                     /translation="MNLLAIFQNKVLIAVLLAWLLAQAMKIPTEYLRSRRWMWAMFFA
                     AGGMPSSHTALMVSGTLAVGLYYGFDNPIFGVAVAATMIIAHDAAGVRSQAGKHAERI
                     NVLFDELLRGHTWSENDLKEVIGHTPLEVVGGIIFGMLVAIVQWMVWK"
     gene            complement(4896..>6129)
                     /gene="murJ"
                     /locus_tag="PLT08_03385"
     CDS             complement(4896..>6129)
                     /gene="murJ"
                     /locus_tag="PLT08_03385"
                     /inference="COORDINATES: protein motif:HMM:TIGR01695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0034204 - lipid translocation [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HNE03536.1"
                     /translation="IVRHVLFVLKPELDPSLQSLTVELLRIILIAPTIFGVSGLLMSI
                     LNTHQKFWLPALAPVFNWVGWILGIIFFVPSMGIYGLAWGYVIGAMLHLLVQVPGLLK
                     LDRVRYSFTLGIGNPAIKEVARLMAPRLLGVAAVQINFVINAMIASGQGEGSLSAINV
                     ARMLMTMPLFVIAQAIATAALPTFSAQVARGEMSEMRSSLAATLRGVVLLSLPATVGL
                     ILLRQPVIVLLFQRGEFDATSTQMVSWALLWYTAGLVGHAVVEILSRAYYALHDTKTP
                     VIVGTIAMGLNALFSFGFAWLFSQAGWMPHGGLALANSFATALEAVALFIIMRKRLDG
                     IEGGSILRGVSASAIAAIAMGLAVVAWMAIGTNLSLWVSAPVGIAIGGLVYFGALFFL
                     RVPELNALVGAVMRRIRR"
BASE COUNT         1567 a         1521 c         1564 g         1477 t
ORIGIN      
        1 gcgaacgata gccccgcttg cggacgtgcc tctactggaa aatgtgaaat ggacacaagc
       61 aaggctggca agtcaagcaa gctggcatct ttcacaggca agccggtcaa tgggttttca
      121 tttgcagcca caagcggcgt ggatgttggg ctgggcaaaa cagtaggttc tgcggtttgg
      181 gttatttcta cagacaaggt tgctgtgttt tcttgttctg aacaagaaaa caacagcgtt
      241 gaaataagag acgcaaaaag agcaagcgcg aaaggtcgtt tggtagtttg cataaaatct
      301 ccaaaccaat attcccccat gatgattaat tccagctaaa cgacgttttg gttatgatga
      361 attgaacgcg ctatggactc gttgataggt ttgctctaga gattcgggta agacgcgcgt
      421 ctcaccgacg gaggacatga agtttgtatc acctgcccaa cgcggcacga tgtgaatgtg
      481 gacgtgcccc aaaacgccgg caccggcggc ttctcccata ttgatgccga cattgaagcc
      541 atgcggctta tagatctggc gcagttccgt ggtgcaacgt gaggtcagtt ccatcatttc
      601 ggcgcgggtc tcgctaacaa gttcttcaat ggtcgccaca tggctgaaag ggatgaccat
      661 taaatgtccg cttgtgtaag ggaagcggtt aaggatcaca taagcggatt ttccacgaaa
      721 cgcgatcaag ttctcggcgc tatccggttt ggattgtgcg ttgcagaata cacagccttc
      781 ttcttttttg ttgccttcaa tgtaattcat acgccacgga gaccatagat gtttcatgaa
      841 aagattctag cacaaacgag attgccatat aaaaattctt gacaagcaga ctgttacggg
      901 tgtataaccg cggtgcaaat tgaatggtcc tcgttaggcc aggcgtctgc aacatacata
      961 agccattgtc ctgaaacgtc gaaagacgcc aaagggtata ccaggtgagg tcggcataag
     1021 gcttcactca atgtggctgg gagcaatcct tacgatgtgc agtgctaaag ctcaaccaga
     1081 gaggcaaggt tttgttttgt gtatgtctgc ctcctggttt tgtaacggga ggctttcttt
     1141 ttgccagcat gtgttgccaa ggcaaatcca accacgtgat gcaaggaggt tatccgtatg
     1201 agtacggata gtagtaaaca gatgcaattt caggcgaaaa gattttcggc ttttcggtct
     1261 gtatcttcct acctccatgc tgttcactgc ccatgagttg accgggtaaa gcataaaacg
     1321 ggggagggaa gctgcaggct tgaacgagtg tgaatcgcct tttcgtcgaa acggaggcga
     1381 ttttttgttg cctccaacag gttctaccca ttttattttt aggagacgac aatgaaccca
     1441 acactcgcaa tgatcaacct tgaagcattg atcgaagcac gtgatttcaa cacattacgc
     1501 gagcagacca agaactggtc agctggcgat ctggctgaat tgatggagcc gctttcggcg
     1561 gagaaagaag cggtcatctt ccgcttgctg ccacgtgaac aagcggcagg cgtattcagt
     1621 tatttatccg aagagcgtca ggaagagtta ttgaaagcca tggcgcgcga agatgtggca
     1681 aacattctaa acgccatgtc gcctgatgac cgtacttcgc tcttggaaga gatgcccgcc
     1741 aatgtgatcc aacatttact gggcttgctt tcggcagagg aacgcacggt cgcttcgcaa
     1801 ttgctgggct atgaagaaga ttcggtcggt cgtttgatga cgcccgactt tgtacgcgtc
     1861 cgcccgcagt ggacgattgc tcaagcactg gaacacatcc gccgctatgg taaagacagc
     1921 gaaacgatga gcatgatcta cgtgatcgat gacaataaca aactggtcga tgatctgcgg
     1981 attcgccaaa tccttttggc atcgccgacc gacaccgtag cggacttgat ggattcgcgc
     2041 tttgtctatt tgaaaacaac agacaatcgc gaaacagccg tagaagcctt taaagactca
     2101 gacttgaacg ccctgcctgt aaccgataaa gaagatacgt tgatcggtat tgttacggtc
     2161 gatgacattc tcgatgtggc agaagaagaa gccacggaag atattcaaaa gatgggtggt
     2221 tctgaagcct tagacgaacc ctacatgaag atcgccattt ccaagatggt gcagaagcgc
     2281 gcaacttggc tgatcatttt attcatcagc gaaatgttga ccgctaccgc catgggaaat
     2341 tttgagcatg aaattgccaa agctgtggtg ctctcgatct ttgtgccatt ggttatctcc
     2401 agcggcggga actccggctc gcaggcttcg acccttatca tccgcgccat ggcgttgggt
     2461 gaagtgcggc tcgtggactg gtggcgcgtg atgcgacgtg agtttttatc cggtttgtcg
     2521 ctgggtgcca ttctcggcgt gatcggcttt atccgcattt ctgtttggca ttaccttttc
     2581 gatgcttacg gtccacattg gttcttatta gcagtaacgg ttggaattgc cctggtaggc
     2641 attgtgttgt ggggttcgct ttccggttcg atgttgccct tccttttgaa gcgcgtcggg
     2701 ctggacccag caacgtcgtc cgcgccgttt gtggcaaccc tcgtggatgt atctggactg
     2761 attatttatt tcaccgtcgc ggcagtgatt ttgaaaggca ctttactcta aacgcatatg
     2821 attcccccta gatgagacag gcgaggaaat tctcgcctgt cttttttatt tgttgcactt
     2881 gctttttttt atgttatcct acctccaatg caaccagact tgttctctgc tacactcacc
     2941 gtctcgcaac tgaccctgcg cattcgcaga ttgttggaag ataaccctga attgcaagat
     3001 gtgtgggtag aaggggagat ttcgaatcta tcacgcccag catcgggtca tatttatttc
     3061 acgctcaaag acaagaatgc ttctttacgt tgtgtcatgt ggaagacaga tgcgctccgc
     3121 actcgtccta gcatgcaaga gggcggtgct gtggaagtgc atggcaagat cgccgtctat
     3181 gaaccgcagg ggcaatatca actcatcgca aatctgattc gtcctaaagg cgagggtgca
     3241 ctctttcaag aatttctccg gcttaaatcc atgctggaag ccgaagggtt gttcgactca
     3301 gatcgcaagc gtgaaatccc tgcatttcca aaaacgattg gcattgtcac ttctgcgacc
     3361 ggcgccgctt tgcgcgacat gctcaacacc attcgccgcc gtcagccact tgcacgagtc
     3421 atccttgcac cgtcgcccgt tcaaggtttg gatgccccgc ccgcgttggt ccgcgctatt
     3481 caagatatca acaggcaaaa tcctgatgtc atccttgtcg cgcgtggggg cggttccatc
     3541 gaagatttat gggcattcaa cgatgaacgc gtcgttcgcg aagtggcgaa ttccaacgcg
     3601 ccggtcatct ctggtgtcgg ccatgaaacg gatttcaccc tcactgattt cgcttctgat
     3661 ttgcgtgccc caactcccac tgcggcggcg gaacttgcga catccaccac attggatgat
     3721 ctccatttac aaatgactag ctaccaaacc cgcctcacag acttaatctc tggcctgctt
     3781 gctgaccatc aagcttccgt ctccgccctt gccgcgcggc tcaagtatgt ctctcccgaa
     3841 cggcgcatcc aagctgaata tcagcacatc gacgaactct cgcgtcgggc tgtttctgcg
     3901 ctgactcacc gcatccaatt acagtctgcc cgcgtagatg gaatatccaa gcggctgggc
     3961 gctttgaatc ccgccggcat tctctcgcgt ggatatgcta tcatcacccg cgaggcagat
     4021 ggcaaagtcg ttagccaggt cttgcaagcg caaggcgata tgaccgtccg tgtgagcgat
     4081 ggcgaattta cagtaaagcg caattcgtag tctacggatt acacgaatct ctttttggag
     4141 caatcatggc aaaagcaaaa cccacccaaa aacctgtcga agagctaacg tatgaagaag
     4201 ctctcgccga acttgagcag accgtggaac tgttggaagg ggagcagaac ccattggacg
     4261 atgcaatgaa gctattcgaa cgcgggcaag ctcttgccgc ccgctgtggt gttttgctgg
     4321 aagccgctca acttaaagta caaaaactag tcggagagac aactttagat tttgaagagg
     4381 aagatgaatg aatcttttag ctatttttca gaacaaagta ttgatcgctg tgttattggc
     4441 ttggctattg gcacaggcaa tgaaaatccc cacagaatat ctgcgttccc gccgttggat
     4501 gtgggcaatg ttctttgccg caggtggaat gccatcttcc cataccgctc tgatggtatc
     4561 tggcacactg gcagtgggtc tgtattacgg ctttgacaac ccgatcttcg gtgtggctgt
     4621 ggctgcaacc atgattattg cgcacgatgc tgctggtgtc cgtagtcaag caggcaaaca
     4681 tgccgagcgc atcaatgtgc tatttgatga gttactacgc ggacatacat ggagcgaaaa
     4741 cgacctcaag gaagttatcg gtcatacgcc attggaagtg gtgggcggca ttatctttgg
     4801 catgttggtt gccattgtgc aatggatggt ctggaaataa aaatcctcct tgaaaaaaat
     4861 cattcctctt tatccagata aagaggaatg attttttaac gccgaattcg acgcatcacc
     4921 gcgccaacaa gtgcattcaa ttcagggaca cgcaaaaaga acaacgctcc gaaataaact
     4981 aacccgccaa tggcaatgcc aaccggagcg gagacccata aactaaggtt ggtaccaatt
     5041 gccatccatg ccacaacggc aagacccatg gcaatggcgg caatggcaga ggcactcaca
     5101 ccgcgcagaa tggagccacc ttcaatacca tccaaacgtt tgcgcatgat aatgaagaga
     5161 gccacagctt caagcgccgt tgcgaaggaa tttgccaacg ccagaccgcc gtggggcatc
     5221 caaccggctt gagagaaaag ccaggcaaag ccaaagctga ataaagcatt caaccccatt
     5281 gcaatcgtcc caactatgac gggagtcttt gtatcgtgca aagcgtaata ggcgcgcgag
     5341 agaatctcta caacggcatg acccaccaaa cccgccgtgt accaaagcaa cgcccacgaa
     5401 accatttggg tcgaggtcgc atcgaactcg ccgcgctgaa agagcaaaac aatcaccggc
     5461 tggcgtaaca gaatcaaccc aacggtggcg ggcaatgaaa gcaatacaac accgcgcagg
     5521 gttgcggcta acgaagaacg catttcgctc atctcaccgc gagccacttg agccgagaag
     5581 gtgggtaagg cggcagttgc aatcgcctga gcaatgacga acagcggcat ggtcatcaac
     5641 atgcgggcga cattgatggc agacaggctt ccttctcctt gacctgatgc gatcatggca
     5701 ttgatcacaa aattgatctg aaccgctgcc acacctaata aacgcggcgc catgagtctt
     5761 gctacttctt taatagcggg gttaccaatt cctaatgtga aggaatatcg cacacgatct
     5821 aacttcaata accctggcac ttgcaccaat aaatgaagca ttgcaccgat aacgtatccc
     5881 catgccaaac cgtaaatgcc catcgaaggg acgaaaaaga taatgccgag aatccagcca
     5941 acccagttga agactggtgc gagcgcaggc agccaaaatt tctgatgtgt gttcaggatg
     6001 ctcatcaaca atccgctcac gccgaagatg gttggcgcga tgagaatgat gcgaagcagt
     6061 tccactgtca gcgattggag ggaagggtca agctcgggct tgagaacgaa tagaacatgg
     6121 cgaacaatt
//
LOCUS       DAOQJE010000083         6105 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_7307_length_6105_cov_7.232397, whole genome shotgun sequence.
ACCESSION   DAOQJE010000083 DAOQJE010000000
VERSION     DAOQJE010000083.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 6105)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6105)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6105
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7307_length_6105_cov_7.232397"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            188..670
                     /locus_tag="PLT08_03390"
     CDS             188..670
                     /locus_tag="PLT08_03390"
                     /inference="COORDINATES: protein motif:HMM:NF023580.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3592 domain-containing protein"
                     /protein_id="HNE03537.1"
                     /translation="MSRIPSLLRIFGWGVFFLVFFLEFAPPAKSYLVTHIGRTLAIIV
                     PFCFSPLVVVGIFGADFMNARLRDTAAKQGHEVMARVVRTSYIGSEGNEYHSYPIIRF
                     ELEVEEDGKVRRASTELIDYGKVDYRNYPPGTQVRAKYDPQTGSIAMLDSRGRVLEKL
                     "
     gene            885..1784
                     /locus_tag="PLT08_03395"
     CDS             885..1784
                     /locus_tag="PLT08_03395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE03538.1"
                     /translation="MFKNKWVLSLALVAILAGLTTTGAFAKGNLPTDQETTAYGIFMP
                     PPPGDFQLSDEALAAAAEVLGITTDELSAALDAGTTLDELATTYSVDIKDVLDAIRAV
                     MPAPANGGFANGQPPANGGQPGQGGQPGQMPLNDEALAAAAEVLGITTDELSAALAEG
                     TTLDELATTYSVDIQDVFDAINTVQPMPQGGPDGQNGQLGQMPLNDEALAAVAEVLGI
                     TTDELSTALDAGTTLDELATTYGVDVQTIFDAINTVQPMPQGGPNGQNGQMPGGQNGQ
                     QPSQGQGGPNGTPPNGQAPGTRP"
     gene            1848..2486
                     /locus_tag="PLT08_03400"
     CDS             1848..2486
                     /locus_tag="PLT08_03400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE03539.1"
                     /translation="MKKNLYSFIFLLLIGALLSACSLPSAAETQSAQPVNDPNAVIPQ
                     SVATASVVTTSSLPAGMPTVETDLSKILSVLQSGNFSYLANLASEKYDASAYTVPGSL
                     VFNAAFPSSQTIYLNYGWCAKDLATLQQNLQHISTYFSFNGQQIPMEYVNTISTESSD
                     GLQCSNTGLLLSGWAPGTYQFQVKASFDSQINDGSADYPAGDYISEYTVTVQ"
     gene            2629..3882
                     /locus_tag="PLT08_03405"
     CDS             2629..3882
                     /locus_tag="PLT08_03405"
                     /inference="COORDINATES: protein motif:HMM:NF024883.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing sensor histidine kinase"
                     /protein_id="HNE03540.1"
                     /translation="MTLISNPSTLKHLEGKEQLLLKLVELSVTLNSTLDLEELLQLIT
                     ATATEALNCEAASILLYDEKHPRLYFAAATGSNPAKLAEIPVPIDNSLAGTIFRTNQP
                     MILNNADQDPRHFPRVAAHINLKVKTLLGVPMPIKDQTMGVLEAVNKRDGSFDESDAA
                     ILSVVAAHAAIAINNARLFRARQQALEKVRAMNEVKNNFLALASHELRTPLGIIIGYA
                     TFLQNDSTGEATEHAGHVLTAASQMRTLLDQMNNLTMLQTDEMILRKMKLPIQDILHF
                     ALDEIKVTASSRDLQLVLAFREDPILVQVDPEKTKLALVNILNNAIRFSPPKSELTIG
                     AIEQGKQVMVWVQDRGEGIPVEKLQKVFEEFYQIEPPNTRQHGGLGIGLTIAKGIVEA
                     QGGSIWAESEGKGRGSTFKVLLPKA"
     gene            3941..4013
                     /locus_tag="PLT08_03410"
     tRNA            3941..4013
                     /locus_tag="PLT08_03410"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3974..3976,aa:Ala,seq:cgc)
     gene            4038..4110
                     /locus_tag="PLT08_03415"
     tRNA            4038..4110
                     /locus_tag="PLT08_03415"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4071..4073,aa:Ala,seq:ggc)
     gene            4583..>6105
                     /locus_tag="PLT08_03420"
     CDS             4583..>6105
                     /locus_tag="PLT08_03420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HNE03541.1"
                     /translation="MVTAKKKNATKKTTTQKAVKKAVKTTAAKTVKKTVAKSAPKAKK
                     PAAKKAATKPSVKKTVKKVSQPRAKKEATPKAVTSKKVQEAPKVKDLSYNPNVIEKKW
                     QAKWDADKLYRSVIDNSKKKHYALTMLPYPSGDLHIGHWYAMTPSDARARFMRMQGYN
                     VLFPMGFDAFGLPAENAAIKSGIHPMVRTRDNIVKMRGQLRSMGAMFDWEREAVSCEP
                     EYYKWTEWFFIQLYKHGLAYRKMSPVDFCPNCNTTLAREQVWGDDRHCERCGTPVIKK
                     DLEQWFFKSTKYADEFLNFDGIDWPQTVKTLQTNWIDRSEGASVIFKTEVGNHDVEIF
                     TTRPDTLWGATFMVFSPEHPLVDEITTPENKAAVDEYKLQAARQSDIQRGAVGKEKTG
                     VFTGSYAINPVNNARIPIWIADYVLMSYGTGAIMAVPAHDERDFAFAKKYELSIIPVI
                     KPEGQSEAVVGETAYIGPGTMVNSGMLDGTKVNTEKGRKNPSIAKVIDWLQEKGVGK"
BASE COUNT         1652 a         1533 c         1505 g         1415 t
ORIGIN      
        1 agagtatcaa aattcaaata aagccgcacc cgttcaaggt ccatgagaga tgcggcattt
       61 tgtcagtcag atgaattgta ggacgttcaa gggcttgggt caagtagaaa atcctgagag
      121 cgtggtacag aaggttgatg ttcaaaaaac tctagcaggg atacgatttg gcaccaggag
      181 attgtatatg agcagaattc catcactatt gagaattttt gggtggggcg tattttttct
      241 ggtctttttt ctggaattcg caccaccggc aaaaagctat ttggtcacac acatagggcg
      301 gacgttggca atcattgttc cattttgttt ttcgcctttg gttgtggtcg gcatctttgg
      361 tgcagacttt atgaatgcac gattgcggga taccgctgcc aagcaagggc atgaagtgat
      421 ggcgcgagtg gtacgcactt catacattgg tagtgaaggc aatgaatatc actcctaccc
      481 gatcattcgt tttgaacttg aagtggagga agatggaaaa gtgaggcggg caagcaccga
      541 attaatagat tatggcaagg ttgattatcg gaactatccg ccagggactc aagtaagggc
      601 aaagtacgac ccgcaaaccg gcagcattgc catgctcgac tcgcgcggaa gggttttgga
      661 aaaactttga tgcaaaaagg ttcctgctga tgaagcagga acctttttta taagctaggt
      721 gagtaaaaaa aatccatcgt ttcatctata tagcctaaac atcttcacag aaacttctca
      781 gtatctgcat agaaaaatca cagcaaatcc acagtctcgg cttctatcat gacggcgttg
      841 gtgagatcgc caacaaatct ttagtgtcaa gaaaaggaga ctatatgttc aaaaataaat
      901 gggtgctcag ccttgctttg gtagctattc tcgcagggtt gaccacaacc ggagcgtttg
      961 cgaaaggcaa cctccccact gatcaagaga caactgcata cggcattttc atgccgcccc
     1021 ctcccggtga ttttcagtta agcgatgaag cgctggcggc tgccgctgaa gtcctcggca
     1081 tcactaccga tgaactttcc gccgctttgg atgcaggtac cactcttgat gagttggcaa
     1141 ccacctatag tgtggatatt aaggatgtac tcgatgcgat ccgtgctgtg atgcctgcac
     1201 ccgccaacgg tggttttgca aacggacagc cgcccgccaa tggtggtcaa cccggtcaag
     1261 gtgggcagcc tggtcagatg cctttgaacg atgaagctct ggcagccgct gctgaagtcc
     1321 ttggcatcac caccgatgaa ctttctgcag ctcttgcaga aggcacaacc ctcgatgaac
     1381 ttgccactac ttatagtgtt gatattcagg atgtgttcga tgccatcaat acagttcaac
     1441 ccatgccgca aggtggaccc gatgggcaga acggtcagct tggtcagatg cctttgaatg
     1501 atgaagcatt ggcagcagtg gcagaagtcc tcggcatcac cactgacgaa ctctccaccg
     1561 cactcgatgc tggcaccacc ttagacgaat tagccaccac ctacggcgtt gacgttcaaa
     1621 ccattttcga tgccatcaac accgtccagc ccatgccaca aggcggtccg aatggacaaa
     1681 acggtcagat gcccggcggt cagaacggtc agcagcccag tcaaggtcaa ggtggtccga
     1741 acggaacacc tcccaatggt caggctcctg gcacacgtcc gtaactcttt ataaaaatgt
     1801 cagcccgcac cgtgcatgcg ggctgacccc tcccataaaa aattcctatg aaaaaaaatc
     1861 tttattcctt tatctttctt cttcttattg gagcacttct aagcgcttgc tctcttcctt
     1921 ctgccgctga aactcaatct gctcaacccg taaatgaccc caatgctgta atcccacaaa
     1981 gtgtggcaac cgcctctgtc gtaacaacct catccctacc cgccggcatg ccaactgttg
     2041 aaacagacct tagcaaaata ctaagtgttt tacagagtgg gaacttctct tatcttgcaa
     2101 atctcgcttc agaaaaatat gatgccagcg catatacggt tcccggctcc ctagtcttca
     2161 atgcagcctt tcccagcagc caaaccattt atctaaatta tggctggtgc gcaaaagacc
     2221 tcgcaacctt acaacagaac cttcaacata tttccacgta cttctctttt aatggtcaac
     2281 aaatccccat ggaatatgtg aacaccatca gcacagaaag cagcgacggg ttgcaatgtt
     2341 ccaatacggg tttactgctt tcagggtggg cgcccggcac ctaccaattc caagtcaaag
     2401 ccagctttga ttcacagatc aacgatggca gtgccgatta ccccgcagga gattacattt
     2461 cagaatacac ggtcaccgtt caataaacca cactcacccc cgaagacagc ctttcgcggg
     2521 tgagttttca tttaattaca gttttattag ccccaactgg tacgaatggt tggttcacaa
     2581 agtataggag gaatctataa ttcagaatga aagtcaagcc aaaacctcat gacccttatc
     2641 tcaaacccca gcacgcttaa acatttggaa ggcaaagaac aacttctctt gaagctggtt
     2701 gagttgagcg ttaccctcaa ctcaactctt gatcttgaag aattgctgca actcatcacc
     2761 gctaccgcta ccgaagccct gaactgtgaa gccgcatcca tcctgcttta cgacgaaaaa
     2821 catccgcgct tgtattttgc tgccgccact ggctcaaacc ctgccaaact ggcagagatt
     2881 ccggtgccaa tcgataacag cctcgccggc acaatcttcc gcaccaacca acctatgatc
     2941 ttgaacaatg cagatcaaga cccgcgtcac ttcccgcggg tggcagctca tattaacctc
     3001 aaggtcaaga ccttgttggg agtgcccatg cccattaaag atcaaaccat gggcgtgctt
     3061 gaagcagtca acaaaagaga cggcagcttc gacgaaagtg atgctgctat tttgtctgtt
     3121 gtcgccgcac atgctgccat tgccattaac aatgctcgtc tcttcagagc acgtcaacaa
     3181 gccttggaaa aagtccgcgc gatgaacgag gtcaagaata attttcttgc tttggcttcc
     3241 cacgagttgc gcaccccctt gggcatcatc attggctatg ccaccttttt acaaaatgat
     3301 tcaaccggcg aagccaccga acatgccgga catgtactca ccgcagcttc tcaaatgcgc
     3361 accctgctcg accaaatgaa caacctgacc atgctgcaaa ccgatgaaat gattctgcgt
     3421 aagatgaagc ttcctattca agacatcttg catttcgcgt tggatgagat caaagtcacc
     3481 gcctccagcc gggaccttca gcttgtgctt gcgtttcgag aagatcctat tctcgtgcag
     3541 gtagaccccg aaaagaccaa acttgctctg gtgaatattc tcaacaacgc cattcgcttc
     3601 tcacccccaa aaagcgagtt gaccattggc gctatagagc aaggcaagca agtcatggtc
     3661 tgggtgcaag atcgaggcga aggtattccc gtagagaaat tacaaaaagt atttgaagag
     3721 ttctatcaga tcgaaccacc gaacacccgt caacatggcg ggttgggaat tgggctgacc
     3781 attgccaaag gaatcgtcga agcgcaaggc ggctccattt gggctgaatc ggaaggcaaa
     3841 gggcgcggtt cgacgttcaa agttctattg ccaaaagcat aatcccttca ttgacattcc
     3901 cttctccctc aggtagaata gccgtcgcct tttgaacgac gggcctgtag cgcagttggg
     3961 agcgcgcctc aatcgcactg aggaggtcag gggttcgaat cccctcaggt ccacagcgtt
     4021 cagaacatca ttttcctggg cccatagcgc agttgggagc gcgtctcaat ggcattgaga
     4081 aggtcggggg ttcgaatccc cctgggtcca cctacgagag cgattaggga actagtcgct
     4141 cttatgctat aatgaaaaaa gttaaaagtt aggacaggag tagtaggtca tcccgtcagc
     4201 gagctgggaa agcgaggtga aagcccagca ttcacgtgcc cacgggagca cataacatga
     4261 ccgaactcgc ctgttgtctc gcaagagaaa ctgcgctggt gaattaagta gtctgcgccg
     4321 ggaatgcccg ttatagcgat caaagagcgt ttgtttattc aaacgaatct gggtggtacc
     4381 gcggagagaa ctcttcgtcc ctgatgtgga tgaagggttt tttgtttctt aagccatcgt
     4441 ctattttgaa aggaggcacg catgaacagt aggcaaattt tttggaagca aggtccgctc
     4501 cttgcggggc ttccgtgtgc atttgtgtaa atcttcaagg tgaagagcct gatagtttga
     4561 accttacatt tgagaggtaa gaatggttac agcaaaaaag aaaaacgcta caaaaaagac
     4621 aacaacccaa aaagccgtaa agaaagctgt gaagacaaca gcggcaaaga ccgttaagaa
     4681 gacggttgca aaatcggcac cgaaagcgaa gaagcctgct gcaaagaaag cggcgacaaa
     4741 gccttcagtg aagaaaaccg tcaagaaggt gagccagccg cgagcgaaga aagaagcgac
     4801 gccgaaggcg gtcacatcca agaaagtgca agaggcgccc aaggtgaaag acttgtcgta
     4861 taaccccaat gtcattgaga aaaaatggca ggcgaaatgg gatgcggata agttgtatcg
     4921 ctctgtgatt gataacagca agaagaagca ttatgcgttg acgatgctgc cctaccccag
     4981 cggtgacttg catatcggtc actggtatgc gatgacgccg tcggatgcgc gggcacgctt
     5041 tatgcggatg caaggctata acgtgttgtt cccaatgggc ttcgatgcct ttggtttgcc
     5101 cgcggaaaat gccgccatta agagcgggat tcaccctatg gtgcgcactc gcgacaacat
     5161 cgttaagatg cgcggacagt tacgctctat gggcgcgatg ttcgattggg aacgcgaagc
     5221 tgtttcttgc gaacctgaat actacaagtg gacggagtgg ttcttcattc agttgtacaa
     5281 gcatggtttg gcatatcgca aaatgtcgcc ggtggatttc tgcccgaact gcaatacgac
     5341 cctggcgcgc gaacaggttt ggggcgatga ccgtcactgt gaacgctgcg gcacgccagt
     5401 tatcaaaaaa gatttggagc aatggttctt caagtccacc aagtatgcgg atgagttctt
     5461 gaactttgac ggcatcgact ggcctcagac ggtgaagacc ctgcaaacaa attggatcga
     5521 ccgcagtgaa ggcgcttcgg tcatcttcaa gaccgaagtt ggcaaccacg atgttgagat
     5581 cttcaccacc cgcccagaca cgttgtgggg tgcgaccttc atggtcttct cacccgaaca
     5641 tccactggtg gatgagatca cgacgccgga aaacaaagcc gctgtggatg aatacaaact
     5701 gcaagcggca cgacagtccg acattcagcg cggtgcggtt ggcaaagaaa agactggtgt
     5761 gttcactggc agttacgcca tcaacccggt caacaatgca cgcatcccga tctggatcgc
     5821 ggattatgtt ttgatgtctt acggtacagg cgcgatcatg gctgtgcctg cgcacgatga
     5881 gcgcgacttc gcctttgcca aaaagtatga gctgtccatt attccggtga tcaagcctga
     5941 aggtcagagt gaagcggtgg ttggcgagac tgcgtatatt ggtcctggca ccatggtcaa
     6001 ctcgggcatg ttggatggaa ccaaggtcaa cactgaaaag ggacgtaaaa atcccagcat
     6061 cgccaaggtc attgactggc tgcaggaaaa aggcgttggc aaaga
//
LOCUS       DAOQJE010000084         6105 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_7308_length_6105_cov_5.904132, whole genome shotgun sequence.
ACCESSION   DAOQJE010000084 DAOQJE010000000
VERSION     DAOQJE010000084.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 6105)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6105)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6105
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7308_length_6105_cov_5.904132"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..245)
                     /locus_tag="PLT08_03425"
     CDS             complement(<1..245)
                     /locus_tag="PLT08_03425"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNE03542.1"
                     /translation="MTTVLLVDDEKLIQRSLEKTLLRAGYDVLTAPDCKTGSEVFAQN
                     ASTVDIAVLDLNMPGFDGMPKNDAGLDLLADLKQKKST"
     gene            complement(270..2195)
                     /locus_tag="PLT08_03430"
     CDS             complement(270..2195)
                     /locus_tag="PLT08_03430"
                     /inference="COORDINATES: protein motif:HMM:NF024883.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNE03543.1"
                     /translation="MNADPRRLPQVLYIADSDESRSLVRRLLAYTFVVLEAANPLDGL
                     GLAEETRPNLILLDTNVAQMTSSEAATRLKKMLSGTPIVVVSGDTSNKARARALAAGA
                     VGFIPKPIGDDFEQLVIEYLNGRTDELENAEQYLREYQLELAEKIEDQTRQLTNTVER
                     NKYLLSQNQKMFAMLERRHKLLETAARVGQMVTSILDMTELLRHTVNIICSEFHFYYS
                     GIFLISDDRKWAVLRAGFALAGRKMLDADYKLPIDDKSMIGRSILTQQAQIALDVAGE
                     ESRFKNPYLPNTRSEMALPLIVNSISVGAVTIQSSELNAFNEEDITSLQAMADQVAIA
                     INNANLMKDLASANAELLRTKTYEAIATATGEAIHWVGNKAAPIPGSVHRVREDVQYL
                     LALVAQLKESELDNAALQAAVETVREEAEAAGLDLKNILAEMSAMKPNRLQALVSVES
                     LMEDLDIAENSAKTILDIKEGLIGPARQRHDAPVSLSEMITNTVDNMGLPHGAVEMKW
                     AEDMPMAHVDARQVEQVFNNLIKNAWEALTLSNIPEPKIYVDGRSDKDANFVLVSVKD
                     NGPGIPKEIQEKIWVSFFTTKGGKGGTGLGLSAVMQIVNQHGGKIWLESEPGQGATFY
                     VSLPAEKLYMPDENTSR"
     gene            complement(2206..3399)
                     /locus_tag="PLT08_03435"
     CDS             complement(2206..3399)
                     /locus_tag="PLT08_03435"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNE03544.1"
                     /translation="MTQPKKKRTPSHAGSPRTQPKAAPQKVHDASPEAIIEMRGVIKK
                     FSNAAGEFTVLKGVDLMINRGEFVSIVGKSGSGKSTLLNMITGIDHPTSGQVIVNHND
                     IYTGVSESARSKWRGKNLGIVFQFFQLLPMLTLLENVMLPMDYVDMYDFDERPIRALE
                     LLELVGLEKFANKLPVLVSTGQQQLAAIARAMACDPPLLIADEPTGNLDTRSSDTIIK
                     LFQHFVDQGKTIVMVTHDPSLTSRTDRNIIISDGEIIDETISRSLPQLRHRHMLEFSK
                     LAQRRMYQPRETIISRDEPVKNFFMIRSGQVEVVLQKQRSKETVLSRLGPNEFFGDIE
                     LLRGGKSIANVRAGSEPVEVVIIPREDFTRVMDESPITVEAISKIVQRRLEQHRMVDP
                     RKSRS"
     gene            complement(3396..4130)
                     /locus_tag="PLT08_03440"
     CDS             complement(3396..4130)
                     /locus_tag="PLT08_03440"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNE03545.1"
                     /translation="MNTPLIELKNVSKTYSTAAGEFPALRNINLNVYAGEFLGVIGKS
                     GAGKSTLLNMINGVDELSEGEVIVNSNNSRVSVHNLSEDEKALWRGKTMGVVYQSFQL
                     LPMLTLVENITLAMDLADNFKPRESRERAMELLRLVEIEEHADKLPSLISGGQQQRVA
                     IARALANDPDILVADEPTGSLDSVTADHIFDVFSRLANERGKTIVMVTHDMGLKSRFS
                     RCLTLVDGELQNEIKADAKPKRSRKQ"
     gene            complement(4203..4514)
                     /locus_tag="PLT08_03445"
     CDS             complement(4203..4514)
                     /locus_tag="PLT08_03445"
                     /inference="COORDINATES: protein motif:HMM:NF024988.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HNE03546.1"
                     /translation="MNDDLRSLTDLDRMIHEPARLLLVTILSGVASADFLFLQRQTGL
                     TKGNLSAHLSRLEEAGYVKIEKTFKGKMPFTVCKLTSAGQKALQRYRQQVQNFMDKTR
                     S"
     gene            complement(4507..5157)
                     /locus_tag="PLT08_03450"
     CDS             complement(4507..5157)
                     /locus_tag="PLT08_03450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE03547.1"
                     /translation="MNQDFNEIQQRVRHSWFKDGVGEIAVGGLFVALSLYFAGHYFAG
                     TRFLPSASNAPLYIDLGLGVVLLLGIVLTRKIIAVFKTHITYPRTGYVEYYIESKNSL
                     LVRVAIFIAGTLFVLLLIWVGRWVGSFKWIPAFLGVFFGGVLLFLRTKTAGLKRFNYH
                     ASLSILLGLGLTFSSMPIPGLSVYYAVFGVWLLGWGIWILAKYLRENPLPEGDEDE"
     gene            complement(5254..5763)
                     /locus_tag="PLT08_03455"
     CDS             complement(5254..5763)
                     /locus_tag="PLT08_03455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE03548.1"
                     /translation="MNDLFTCADELSLAVKVAADSPDIYLRIGEPDDVLTPVFQIDEE
                     ELLIVTHRESPVQNLSLEETQALFAGSSGLAVQVWVYPSELDVYEVFDQFVMQGRSVI
                     SAARVAVNPQEMSDVLNQESNAVGILPRHWKAGNVREVYSVGIFPVLALTQEEPQGNV
                     AQLIACLQK"
     gene            complement(5902..>6105)
                     /locus_tag="PLT08_03460"
     CDS             complement(5902..>6105)
                     /locus_tag="PLT08_03460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE03549.1"
                     /translation="IAAEIGIDLKAGGTGGVSDANLVAPLGIPVLDGLGAIGEGYHSE
                     REYIFAGSVAQRVKLISALLVNW"
BASE COUNT         1673 a         1543 c         1295 g         1594 t
ORIGIN      
        1 gttgatttct tttgcttgag atctgccaaa aggtcgagcc cggcatcatt tttaggcata
       61 ccatcaaaac cgggcatatt caaatccagc acggcaatgt caacggtgct ggcgttttga
      121 gcaaagacct cactgcctgt cttacaatcg ggcgcagtca acacatcata cccagcccgc
      181 aagagagtct tttccaaact gcgttggatc aacttctcat cgtccactaa aagaactgtc
      241 gtcatcattc ctccaaagtt gtacacgcat tagcgtgaag tattctcatc tggcatatat
      301 aacttttccg caggcaggct cacataaaat gtagcacctt gcccgggttc actttccaac
      361 caaatcttgc caccatgttg attcacaatc tgcatcactg ccgaaagccc caagcccgtg
      421 ccacctttac cgcctttggt tgtaaagaac gacacccaaa ttttctcctg tatctctttc
      481 ggaattcccg gtccattatc ttttactgaa acgagcacaa aatttgcatc tttatcgctg
      541 cgcccatcaa cataaatttt aggctccggt atatttgaaa gagtcaacgc ttcccaagca
      601 ttcttaatga ggttattgaa aacttgctcc acctggcgcg catccacatg tgccatgggc
      661 atatcttctg cccatttcat ttccacggcg ccatgcggca agcccatatt gtctaccgtg
      721 ttcgtgatca tctcgctcaa agaaaccggc gcatcatgtc gctgacgagc aggtccgatc
      781 aaaccttctt tgatatcaag gatggtcttt gcgctatttt cagcaatatc taaatcttcc
      841 atcaaagact caacgctgac caacgcctgc aagcgattcg gcttcatggc agacatctct
      901 gccagaatat ttttcaaatc caagcctgcc gcttccgcct cttcccggac cgtttcaacg
      961 gcggcttgca acgcggcatt atccaactct gattccttta actgcgcaac caaagccaat
     1021 aaatattgaa catcttcacg gacacgatgc acacttccag gaatcggcgc agccttgttc
     1081 ccaacccagt gaattgcttc cccggttgct gtagcaattg cctcatacgt ctttgtgcgc
     1141 aacaattcgg catttgcaga tgccaggtct ttcatgagat tggcattgtt gatagcaatt
     1201 gcaacctgat ctgccatagc ttgtaaagac gtaatatctt cttcattgaa agcattaagc
     1261 tcactgcttt gaatagtcac cgcaccgaca gagatcgaat tgacaataag tggcaatgcc
     1321 atctctgagc gagtgttcgg caaatacggg ttcttgaagc gtgactcctc acccgccaca
     1381 tccaatgcaa tctgagcttg ttgtgtcaag atcgagcgtc cgatcatgga tttatcatca
     1441 atcggcaatt tgtaatcggc gtccagcatc ttgcgcccag ccaatgcaaa ccctgctcgt
     1501 agaaccgccc acttacggtc gtctgagatc aggaaaattc cagaataata gaaatggaac
     1561 tcactacaaa taatattgac ggtgtgccga agtagttctg tcatatccag aatagatgtg
     1621 accatctgcc ctactcgtgc cgccgtttcc aataacttat gtctgcgctc aagcatggca
     1681 aacatcttct ggttctgtga aagcagatat ttgttgcgtt caactgtatt cgttagttga
     1741 cgggtctggt cttcgatctt ttcggctaat tcaagttgat attcacgcaa atactgttca
     1801 gcattttcta attcatcggt tctgccgttc agatattcaa taaccagttg ttcaaagtca
     1861 tcgccaattg gcttgggaat gaagcccacc gctccagctg ccaaagcccg cgcacgtgcc
     1921 ttattcgatg tatctccaga aacaaccaca atgggagtac cagacaacat tttcttaagg
     1981 cgagttgctg cctcactgct tgtcatctga gcgacattgg tatctagcaa aatcaaattt
     2041 ggacgggttt cttcagccag tcctaaccca tctaacggat tcgcagcctc taacacaaca
     2101 aaggtatatg caagcaaacg ccgcacaagc gaacgagatt cgtcgctatc ggcgatatat
     2161 aaaacttgcg ggagtctgcg agggtcagcg ttcataggag caaatctacg agcgactctt
     2221 tcttgggtct accatgcgat gttgttccaa acgtctctga acgatcttgc tgatggcttc
     2281 tacggtgata ggagattcat ccataacacg cgtgaaatct tcacgtggaa tgatcacgac
     2341 ctcaaccggc tcgcttcctg cgcgcacatt cgcaatcgat ttgccgccgc gtaagagctc
     2401 aatatcacca aaaaattcat taggtcccaa acgcgaaagg acggtttctt tgctgcgttg
     2461 tttttgtaaa acaacttcta cttgtccgct acggatcatg aaaaagtttt tcacgggctc
     2521 atcgcgagaa ataatggttt ctctcggctg atacattcgg cgctgggcaa gtttggaaaa
     2581 ctccagcata tggcgatgcc gaagttgcgg caaagagcgg gaaatcgttt cgtcaataat
     2641 ttcgccgtca gagatgatga tgttacggtc tgtacgcgag gttaaggatg ggtcatgggt
     2701 caccatcaca atggtcttgc cttggtcgac aaaatgctga aacaatttga tgatggtatc
     2761 agaagaacga gtatcgaggt tgccggtggg ttcatctgca atcagtagcg gagggtcaca
     2821 tgccatagca cgtgcaatcg ctgctaactg ttgttgaccc gttgaaacca aaaccggtag
     2881 tttattagcg aatttctcta aacctactaa ttccaaaagc tccaaagcgc ggataggacg
     2941 ttcatcaaaa tcgtacatgt ccacataatc catcggaagc atgacgtttt cgagtaaggt
     3001 caacatgggc agtaattgga agaattggaa aacaatgccc aagttctttc cgcgccattt
     3061 tgagcgagcg ctctcactca cgccggtata aatatcattg tggttgacaa tgacttgtcc
     3121 gctggtggga tggtcaatgc ctgtgatcat atttaatagc gtcgatttac cgctgccaga
     3181 tttacccaca atagacacaa actcaccgcg gttaatcatc aagtctacgc ctttgaggac
     3241 ggtaaactca ccagcggcat tggaaaattt cttaatcacg ccgcgcattt cgatgatcgc
     3301 ttccggtgag gcatcatgca ctttttgtgg agcagctttc ggctgagtcc gaggcgaccc
     3361 ggcatgcgag ggagtgcgtt tcttcttcgg ttgcgtcatt gcttgcggct cctcttcggt
     3421 ttggcatccg ctttgatttc attttgtaat tcaccatcga cgagagtcaa gcaacgagaa
     3481 aagcggctct ttaagcccat gtcatgcgtt accatcacaa tggtcttgcc acgttcattt
     3541 gccagacgcg aaaagacatc aaagatgtgg tctgcggtga cagagtcgag gctgccggtg
     3601 ggttcatcgg caacaagaat atcggggtcg ttcgccaatg cgcgcgcaat tgcaacacgt
     3661 tgctgctgac cacccgagat gagggatggg agtttatcgg catgttcttc gatctctacc
     3721 aaacgcaaaa gttccatagc gcgttcacgc gattcgcggg gtttgaaatt atctgccaga
     3781 tccatagcaa gggtgatatt ctcgaccagg gttagcatgg gtagaagctg aaaggactgg
     3841 tacacaacgc ccatagtttt accgcgccag agtgcttttt catcttcact caggttatgc
     3901 acagaaacac gagaattgtt cgagttgacg atcacttcgc cttcgctcag ttcatctact
     3961 ccgttgatca tgttcaacaa ggtagatttt cccgcgcctg atttaccaat tacccctaga
     4021 aattcgcccg catagacgtt taggttgata ttccgcaaag cggggaattc gcctgccgcc
     4081 gtagaatatg tttttgatac attcttcaat tcaatcaatg gtgtattcat aagttcaatt
     4141 tcattttacc gctgttgttg atgcgatttt gtgtccttta tttgcaaaga gttttcaatt
     4201 gctcacgaac gggtcttatc catgaaattt tgtacctgtt ggcgatatct ctgtaacgcc
     4261 ttttgcccag cactggtcag cttacaaact gtgaagggca tttttccttt aaaggtcttt
     4321 tcgatcttca catacccggc ttcttccaaa cgcgaaagat gggcagacag gtttcctttg
     4381 gtcaaaccgg tctggcgttg gagaaagaga aaatcggcgc tggcaacgcc ggagagaatg
     4441 gttaccaata agagccgagc cggttcgtga atcatacgat ctagatcggt tagtgaacgg
     4501 aggtcatcat tcatcttcat ctccctctgg cagtgggttc tcacgcaaat actttgccaa
     4561 gatccaaatc ccccaaccta acagccaaac gccaaaaaca gcgtagtaca cactcaaccc
     4621 agggataggc atactgctaa atgttagtcc aagtcctaat aagatggaca aacttgcatg
     4681 atagttaaaa cgtttcaagc cagctgtctt tgtgcgcaga aataacaaga ctccgccaaa
     4741 gaaaacgccc agaaaagcgg gaatccactt gaaagacccc acccatcttc caacccaaat
     4801 caataacaga acaaataatg tacctgcgat aaaaatagca actcgtacca ataaagagtt
     4861 cttagattca atgtagtatt ctacataccc tgtacgtgga tacgtaatat gagtcttgaa
     4921 cacagcgatg atcttgcgtg ttagcacgat gcctaaaaga agaacaactc ccaaaccaag
     4981 gtcaatgtac aacggcgcat tggaagccga aggcaaaaag cgtgtgccag caaagtagtg
     5041 cccggcaaaa tatagtgaaa gtgctacaaa taatccgccc actgcaattt cgcccacacc
     5101 atccttaaac caggagtgcc gcacacgctg ttgaatttca ttaaagtctt gattcataat
     5161 actccacaca aaccggtttg cactacaaac cggtttagat aataccatcc cattaggagg
     5221 cgtcaagtga aaaataaaac taagatgaga attttatttc tgcaaacaag cgattaattg
     5281 agcaacattc ccctgcggct cttcttgagt caaagcaaga acgggaaaaa tccccacaga
     5341 atacacctca cgcacattcc ccgctttcca atgtcgcggc aggattccga ccgcattcga
     5401 ttcttgattt aacacatcag acatctcttg cggattcacc gccacccgag ctgcagatat
     5461 cacgcttcgt ccttgcatca cgaactggtc aaacacctca tacacatcca actctgatgg
     5521 atacacccaa acctgtaccg ccaatccact tgaaccagca aataaagctt gagtttcttc
     5581 taaagaaagg ttttgcaccg ggctttcacg atgcgtgaca atgagtagtt cttcttcgtc
     5641 aatttgaaat acaggggtta gtacatcgtc tggctcaccg atacgcaaat aaatatcggg
     5701 cgagtctgcg gcaactttca ccgcaagaga cagttcatcc gcacaggtaa ataagtcatt
     5761 catccatggc tgcgcagatg ctgtggaata cgctgtgata atctgatttg aagataatgg
     5821 ttttggtgca caggaaaaca aaaagaagga aagacaaaca aaaataatct tcttgttata
     5881 tggaacagcc atagaaaatg attaccaatt gacaagcaat gccgagatca actttacacg
     5941 ttgagccaca ctacctgcaa aaatgtattc gcgctctgaa tgatagcctt cgccaatcgc
     6001 accaagacca tctaagaccg gaattcctaa tggcgcgacc aaattcgcat cagagacgcc
     6061 gcctgtacca cccgctttga ggtcaatgcc gatctcagca gcaat
//
LOCUS       DAOQJE010000085         6076 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_7355_length_6076_cov_6.613353, whole genome shotgun sequence.
ACCESSION   DAOQJE010000085 DAOQJE010000000
VERSION     DAOQJE010000085.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 6076)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6076)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6076
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7355_length_6076_cov_6.613353"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..233
                     /locus_tag="PLT08_03465"
     CDS             <1..233
                     /locus_tag="PLT08_03465"
                     /inference="COORDINATES: protein motif:HMM:NF024769.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="YdeI/OmpD-associated family protein"
                     /protein_id="HNE03550.1"
                     /translation="LNAEFIIPPDILKAIQADKEAWKNFQNFSDVYIRIRVAFIDSAR
                     KRPDEFKKRLRYLIEMTAKNRQIGFGGIEKHY"
     gene            230..388
                     /locus_tag="PLT08_03470"
     CDS             230..388
                     /locus_tag="PLT08_03470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE03551.1"
                     /translation="MTPTRWHPDILDLSCLVQELVTFALLAVEPIAGQTVACPCALYV
                     RSRLILEM"
     gene            complement(451..4956)
                     /locus_tag="PLT08_03475"
     CDS             complement(451..4956)
                     /locus_tag="PLT08_03475"
                     /inference="COORDINATES: protein motif:HMM:NF024583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HNE03552.1"
                     /translation="MLKTIKQFFTPPVFPDEDDKTRNAQILYALFASMMGILALLMLA
                     NIFVFVAKAIVSILLFILFIWILVARALAQSGKILLASRIFVSGLWILTTFQLLLSGQ
                     LSAAALAFHVSNLSIAGVLLKKRSAITLAVMSSLVGLGMVILEITGYSLHRYFPNPPI
                     VYWITWVIAFIMTIIPLNLTVQSAGDALARAQKSERRYVTLFNKAPMMYLIIRNEGDI
                     PIISDCNFTFLKNLGYTREQVIGQPLANFYTPQSRLMLLKEGGFQSSFQNEVTTSIER
                     DFVAQDGHILHTLVRTASEIDESGQSIGVLAMYLDITERKQAEEALRESETKLQTIFA
                     RSRDAIGVSKSGVHIFVNPAYVFMFGYESAEELIDTPVINLIAPESREMIIHNIQDRD
                     KGLAVPSDYETVALRKDGSTFILDVRISTYILQGEQYTLVILRDITERKRAEEALQIK
                     EWAIRSSINALALSDLDGSLIYVNPAFLALWGYQTEQEVLGRSAIEFWQVQENADQVI
                     SALRTQGGWMGEMVATRKDGTLLDVEVVASMVADNAGKPLCMMGAFVDITDRKRAEVE
                     LTIAKEKAEENEKKLREVLDNTKVQLWAFDGTRYNYVSKEWYDFTGQTLDTPLTIDLW
                     VSAVHPDDREPSGKIWLENWERKTAHDNYFRLRRQDGVYRDFYCHAIPIFDSQNKFKY
                     FHGFNVDITERKQAEEKLRHAENRLELFFSQSLDGFFFMLLDEPIQWSDDIDKDKTLE
                     HVFAHQRITKANDAMLSLFGTTRKQFIGLTPKDLYPGDVEQRKHFWGDLFDKGHLQLL
                     TAETKSDGSIMWLEGDYVCMYDTDGRLLGHFGIVRDVTERKQAEAVTAARARILQFSL
                     THSLPDFLQNVLDECELLTSSSIGFFHFMEADQNTLSLQNWSTRTLGEFCTAVGKHLH
                     YPLSQAGVWADCARERQAIICNDYAGAINSKGMPDGHAEIKRFVSIPILRGENVVAII
                     GIGNKAVDYDREDVEIVTQLADLSWDITERKRAEDSLWESETRFRRLANNAPDIIFRY
                     NFTPEMKLAFINPAVEKITFYTPEECYADPLLMLNMAHPDDAMMMAEYLQSRQTPDKP
                     LIMRWLDKYGTIHWMESRLVPIYDEADQLVAVEGITRDITERKRAELQIEEQLKRLNA
                     MHTIDTFIKSSFDLKNTLNILLTEVATQLKVDAASILLFNKDTLTLNYSAGYGFRSIA
                     IQHTKLRYGEGFAGRLLHDRKIIRIPDLTKTESELAKALALKGDNFTSYMGVPLIAKG
                     QTLGVLEIFQRAELTPDTDWFDFLEMLADQAAIAIDNMQLFESLQRSNLDLTLAYDAT
                     IEGWSRALDLRDKETEGHTQRVTSLTIQLAQQMGVPDADILHIRRGALLHDIGKMGVS
                     DNILRKPGPLTEAEWQEMRQHPLYAYKMLAPISYLNPALDIPYCHHEKWDGTGYPRGL
                     KGEEIPLVARIFAIADVYDAITSDRPYRAAWAKEKALDFIREQSGRHFDPNVVKYFLE
                     MIKDEVK"
     gene            complement(5091..5492)
                     /locus_tag="PLT08_03480"
     CDS             complement(5091..5492)
                     /locus_tag="PLT08_03480"
                     /inference="COORDINATES: protein motif:HMM:NF013970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HNE03553.1"
                     /translation="MTDYVADTHALIWYLENSPRLGSQARVAFDACDRGEIQIYVPTI
                     CLVEIVYLMEKGRISPEMKSMLDEELKSGESGLVLADLNMDVVDQIANVLRDDIPDLP
                     DRVIAATALALNLPLISRDRKIVLSGLDTIW"
     gene            complement(5492..5722)
                     /locus_tag="PLT08_03485"
     CDS             complement(5492..5722)
                     /locus_tag="PLT08_03485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE03554.1"
                     /translation="MVTPTSTDVLKMLRQLPPSEQLRIISLALPEIEKSLGRKARARK
                     SLCGLWSGAGITPKDIAETHKGMLSNFPRSDI"
     gene            complement(5751..>6076)
                     /locus_tag="PLT08_03490"
     CDS             complement(5751..>6076)
                     /locus_tag="PLT08_03490"
                     /inference="COORDINATES: protein motif:HMM:NF012398.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HNE03555.1"
                     /translation="QKYPLSAMDFYQVLDTSDVPGGVVNIVTGDRDHLVKTLVQHEDV
                     DSVWYFGSAEGSYHVENESAANMKRTWTGYGLPRDWLDREQGEGHEFLHEATQVKNIW
                     VPTGE"
BASE COUNT         1535 a         1525 c         1425 g         1591 t
ORIGIN      
        1 tcctgaacgc cgagttcatc attccacccg atatcttgaa agccattcaa gcggataaag
       61 aagcctggaa gaattttcag aacttttccg atgtgtacat tcgcatccgc gtggcattta
      121 ttgatagcgc acgtaaacgc ccggatgaat tcaaaaaacg attacgatat ttaattgaaa
      181 tgaccgccaa gaacaggcag atcgggtttg ggggaattga gaagcattat tgacccccac
      241 ccgttggcac ccagatattc ttgacctgag ttgcctcgtg caggaactcg tgaccttcgc
      301 cttgctcgcg gtcgagccaa tcgcggggca gaccgtagcc tgtccatgtg cgctttatgt
      361 tcgcagtaga cttattctcg aaatgtaact accttcctct gagcaatcag gtttgatgta
      421 aacacatata actattttgc aaataaaagt ttatttcact tcatccttta tcatttccaa
      481 gaaatacttg acaacattgg ggtcaaagtg cctgccgctt tgctcacgga taaaatccag
      541 agctttttcc tttgcccacg cggcacgata aggacggtca ctagtgatgg catcgtacac
      601 atccgcaatg gcaaagatac gcgccactag tggaatttct tcgcctttca aaccgcgcgg
      661 ataccctgtg ccatcccatt tttcgtggtg acaatagggg atatccagtg cggggttcaa
      721 gtaagagatg ggggcgagca ttttatacgc atatagagga tgttggcgca tttcctgcca
      781 ttccgcctcg gtcaatggac caggcttacg caggatatta tccgacacac ccattttgcc
      841 aatatcgtgg agcaatgcac cgcggcggat gtgaagaata tcggcgtcgg gaacgcccat
      901 ttgctgtgcc agttgaatgg tcaaggatgt aacacgctgg gtatgcccct cggtctcctt
      961 gtcgcgcagg tccaatgcgc gcgaccagcc ttcgatagtg gcatcgtaag ccaaggtaag
     1021 gtcaagattg gagcgttgca ggctttcgaa taattgcatg ttgtcaattg caatggctgc
     1081 ctgatctgca agcatttcaa ggaagtcaaa ccagtcagtg tctggggtga gttcagcgcg
     1141 ctggaagatt tccagtacac ccagggtttg ccccttggca atcagcggca cgcccatgta
     1201 agaagtaaaa ttatcacctt tcagtgcaag ggctttggca agttcgctct ctgttttcgt
     1261 caggtctgga atgcggatga ttttcctgtc atgaaggagg cggcccgcaa agccttcgcc
     1321 gtatctaagt tttgtgtgct gtatggctat agatcggaac ccatagccgg cggagtagtt
     1381 aagcgtaagt gtatccttgt tgaataaaag tatggaggcg gcatccacct tgagctgggt
     1441 ggcaacctca gtcagcaaaa tattgagcgt gttcttcaag tcaaaactgg atttgatgaa
     1501 agtatcaatg gtgtgcatgg cgttcagccg cttgagttgt tcctcgatct gtagttctgc
     1561 ccgttttcgt tcagtaatgt cacgggtgat accttccacc gccacaagtt gatccgcttc
     1621 atcgtaaatc ggcacaagac gactttccat ccagtgaatt gttccgtatt tgtccagcca
     1681 gcgcatgatc aaaggcttgt caggagtttg gcgcgattgc aaatactcag ccatcatcat
     1741 tgcatcatcg ggatgcgcca tattgagcat taacagtggg tctgcataac attcctccgg
     1801 ggtgtagaag gtgatttttt ccactgccgg gttaataaaa gccagcttca tctctggggt
     1861 aaagttataa cggaaaataa tatcgggggc gttattggcc aaccgtcgga aacgagtttc
     1921 gctctcccac aacgaatctt ctgcccgttt gcgctcggta atatcccagc taaggtctgc
     1981 caattgggtc acaatctcga catcctccct atcatagtcc acggctttgt taccaatgcc
     2041 aatgatggct acaacgtttt cgccgcgcag gatgggaatc gacacaaaac gtttgatctc
     2101 cgcatgcccg tctggcatgc ctttgctgtt tatcgccccg gcataatcat tgcaaatgat
     2161 ggcttgtcgt tcacgggcgc aatcagccca gactcccgcc tgacttaatg gataatgcag
     2221 gtgtttgcct acagcagtac aaaactcacc caaagtgcgt gtggaccagt tttgtagcga
     2281 caatgtattc tggtcggctt ccatgaaatg gaaaaaaccg atcgaactgg atgtgagcaa
     2341 ttcacattca tcgaggacgt tttgcaagaa atctggaagc gaatgtgtca gtgaaaattg
     2401 caagatgcgc gcgcgtgcgg cagtaaccgc ctctgcctgc ttgcgttcgg tgacatcgcg
     2461 tacgatgcca aaatgcccca aaagacgtcc atcggtatca tacatgcaca cataatcccc
     2521 ttcaagccac atgatggaac catcagactt ggtctcagcg gtaagcagct gaagatgtcc
     2581 tttatcaaaa agatcacccc aaaaatgctt cctttgttcg acatcgccgg gataaagatc
     2641 tttcggtgtc agcccaatga actgcttacg agttgttcca aacagagaca acatggcatc
     2701 gtttgctttt gtgatgcgct gatgagcgaa aacatgctcc agtgttttat ctttatcgat
     2761 atcatcactc cattgaatcg gctcgtctaa aagcatgaag aaaaagccat caagagactg
     2821 tgagaaaaaa agttcaaggc gattttccgc atggcgcaat ttctcctccg cttgtttgcg
     2881 ttcagtgata tccacattaa agccatgaaa gtatttgaat ttattttggc tatcgaaaat
     2941 cggtatggca tggcaataaa agtccctata tacgccatct tgtcttctca atcgaaaata
     3001 attatcgtgc gcggttttcc tttcccaatt ttccaaccat attttccctg aaggttcgcg
     3061 gtcatcagga tgtactgccg aaacccacag atcaatggta agcggggtgt ctagtgtttg
     3121 cccggtgaaa tcgtaccatt ccttgctcac gtagttatac ctggttccat caaaagccca
     3181 caattgaact ttggtgttgt ccaacacctc gcgtaacttt ttttcatttt cttctgcctt
     3241 ctctttggca atggttaatt ccacttccgc ccgcttacgg tccgtgatgt cgacaaatgc
     3301 acccatcatg caaagcggtt tgccggcatt gtctgcaacc atgctggcta ccacttcaac
     3361 atcaagcaat gtgccatctt ttcgcgtggc taccatctcg cccatccagc cgccttgagt
     3421 tcgtaatgct gagatgactt gatccgcatt ttcttgtacc tgccagaact ctatagccga
     3481 cctgcctaga acctcctgct cggtctggta tccccacaaa gcaagaaaag cggggttgac
     3541 gtatatcagg ctcccatcca agtcagatag ggcaagggca ttaatggaag accggatagc
     3601 ccactctttg atttgcaacg cctcctctgc ccgcttgcgc tcggtgatgt cgcgcagaat
     3661 gactagtgta tattgttcgc cttgcaatat gtaggtggaa atacgcacat ccaaaataaa
     3721 agttgatccg tcttttcgga gcgcgacggt ttcataatca gaaggcactg ctaaaccttt
     3781 atcacgatct tgaatattat ggatgatcat ctcgcggctt tcaggcgcga tgagattgat
     3841 aacgggagtg tcaatcaact cctccgcgct ttcatatccg aacatgaata catacgccgg
     3901 attgacaaaa atatgaaccc cgcttttgga aaccccgatt gcatccctcg atcttgcaaa
     3961 gatagtctgt agtttcgttt cgctttcgcg cagcgcctct tccgcctgtt tgcgttctgt
     4021 tatgtccaga tacatcgcca gcacaccaat ggattgcccc gactcatcaa tctcagaagc
     4081 agtccgtacc agtgtgtgga ggatatgccc atcttgcgcc acaaaatccc gctcaatcga
     4141 cgttgtcacc tcattttgga acgaactctg aaaaccacct tctttcaata gcatcaagcg
     4201 tgattgggga gtatagaaat tagccagtgg ctggccgatc acttgttcac gcgtatagcc
     4261 caggtttttt aggaatgtaa aattgcaatc cgaaataatg ggtatgtctc cctcattgcg
     4321 aattatcaag tacatcatcg gcgctttgtt gaacagggtc acgtagcgtc gttcgctctt
     4381 ctgcgcgcgt gccagcgcat ctcctgcgct ctggacggtc aggttcagcg ggataatggt
     4441 catgataaaa gcaatgaccc aggtgatcca atatacaatt ggggggttgg gaaagtaacg
     4501 atgcaacgaa taacctgtga tttcaagaat taccatcccc aacccaacca ggctgctcat
     4561 caccgccagt gtgatggcag aacgcttctt taaaaggaca ccagcaatgg acaagttcga
     4621 cacatgaaaa gcgagagcgg cagcagatag ttgtccagac agtaaaagtt ggaatgtagt
     4681 tagaatccac aatccgctca caaatatccg gctggcaagc aggattttgc cgctttgcgc
     4741 cagtgcgcgt gcaaccagta tccaaatgaa aaggataaac agcaaaatac tcacaatggc
     4801 tttggcaaca aatacaaaaa tgttggcaag cataagaagt gccaaaatcc ccatcatgct
     4861 agcaaatagc gcatagagta tctgcgcatt acgggtcttg tcatcctcat ctgggaagac
     4921 tggcggcgta aagaattgct tgatggtttt tagcataggg cacctcttat tttgaaacaa
     4981 gggtactttt gttgggcgtg cttcgcgcag ggctatctat tcacagagta gttagcgaca
     5041 ggggtcactc ccccgtcggc acccagatat tcttcacctg cgtcgccaaa ttaccaaatc
     5101 gtatccaaac cactcaaaac aatttttctg tcacggctaa tcaagggtaa atttaatgca
     5161 agtgcggttg ccgcaataac ccgatctggc aagtcaggaa tatcatcacg caggacgttg
     5221 gcaatctggt caactacgtc catattgagg tctgcaagta caagtccgct ttcgcctgat
     5281 ttcaattcct cgtccaacat ggatttcatt tcaggggaaa tgcgtccttt ttccatcagg
     5341 tagacaattt caaccagaca gatggtcggc acgtaaattt gaatctcacc tcggtcgcat
     5401 gcatcaaaag cgacgcgggc ttgtgagcca aggcgagggc tgttttccaa ataccagatc
     5461 agagcatgag tatcggcgac gtaatcagtc atcaaatatc actccgcggg aagttgctca
     5521 acatcccctt gtgtgtttca gcaatatcct tgggagtaat accagcaccc gaccacaatc
     5581 cgcacaggga tttgcgtgcg cgggctttcc tgccgaggct tttctcgatc tctggcaagg
     5641 caagcgaaat aatcctcaac tgttcgcttg gtggtagttg acggagcatt ttcaaaacgt
     5701 cggttgatgt tggagtaacc ataaatcacc tcacagaata attatacctg tcactctccc
     5761 gtcggtaccc agatattctt gacctgagtc gcctcgtgca ggaactcatg accttcgccc
     5821 tgttcgcggt cgagccaatc gcggggcaga ccgtagcctg tccatgtgcg cttcatgttc
     5881 gcggcggact cgttctcgac gtggtaactg ccttccgccg agccgaagta ccagactgag
     5941 tccacgtctt cgtgttggac gagggtcttg accaaatggt cacggtcgcc tgtgacgatg
     6001 ttgaccacac cgccaggcac gtcggacgtg tcgagcacct gatagaagtc catggcggag
     6061 agcggatatt tctggc
//
LOCUS       DAOQJE010000086         6067 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_7370_length_6067_cov_5.118762, whole genome shotgun sequence.
ACCESSION   DAOQJE010000086 DAOQJE010000000
VERSION     DAOQJE010000086.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 6067)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6067)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6067
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7370_length_6067_cov_5.118762"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1385)
                     /locus_tag="PLT08_03495"
     CDS             complement(<1..1385)
                     /locus_tag="PLT08_03495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE03556.1"
                     /translation="MKRILEIFKINPGEERMSTLLVGLMLLTSAGSALGGNAVSALFF
                     NRFGVESLPVMYMLLGTLTFMTSMGITIIMGRFSKKHLFTYLPVILGMVLLAGWITLG
                     LGIKWIFAVMWLGMNVIGSLQGVWVWGLAGAACDTRQAKRLFPLFSAGGILGAVLGGF
                     LTPPLAGRLHSETLVLLWAGVFLLCFYLGRTLVGGAVPETSSRAGNANILTEMQRGYQ
                     FVRRSSIMQWVAYSAFLFSLCYFFLDLQFSRGASARFPSADQLAGFLGLFQSINTIIA
                     LIVSLFLANRLFARFGIMPMLLLFPVIYLLGFGVLSFFAPFPALVSVRFLQMVWMQGI
                     AGTAWQTLFNVVPANQRDQVRTFIGGAPEQLGVFAAGLLLFLGDRALNSQQLNIVALF
                     SAAALVYVIWRASKAYGLSLVDALREGQPQIFFSEEQPFGGFHTDAAAVAVVLQGADD
                     PDVSIRRASTD"
     gene            1493..4303
                     /locus_tag="PLT08_03500"
     CDS             1493..4303
                     /locus_tag="PLT08_03500"
                     /inference="COORDINATES: protein motif:HMM:NF012437.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HNE03557.1"
                     /translation="MSGLHSGTVTFLFTDIEGSTRLWEEHPEAMKTALAKHDSVLRES
                     IESSHGQIIKTTGDGVHAVFETAIDSIRASIAAQSGFQTPEFLNKSGVLLRVRMGIHT
                     GEAELRDGDYYGSVLNRAARLMSMAHGGQILISNATSELIRDGLPANVTLRDLGEHRL
                     RDLTRPEHIFQLVHPDLPGEFPPLKTLDAFPNNLPVQLTSFIGREKEIAEIKSALGSA
                     RLVTLTGSGGTGKTRLSQEVGMQLLTNFQHGVWLVELAPLSDESQIIPALAQVFGLQE
                     LPFNPLAKLVEDYLRDKKLLLILDNCEHLIAGCARLADHLLHQCAGLKILASSREALG
                     IAGEVAYRTPSLSSPALGGFPAAEDGGLSKKILEYESTSLFVDRARAANSKFALTDSN
                     ASSIAQICHRLDGIPLAIELAAARTKLLSAEQIATRLDDMFRLLVGGSRTALPRQQTL
                     RALIDWSYDLLSDEEKRLLQFASVFVGGWTLEALEFVAEEPSTLELLEQLVNKSLVVT
                     EEHKGEMRYFLLETIRQYGREKLFEAKQASAARDRHFIYYKNLTNGLWDVTYFASEGQ
                     TQRLISQQAEVENLRAAFEWGLQNDAQDALELAANIAMSMSMMGGQSEGTNMLKTAME
                     KFRALPPAAGGDAERVRKEIYAHGCFSLGMLLQGTNEITYSRNTLQEAIAIARELGDK
                     HMLGIALEMYANASAMIKADDTVTAAQEGLEIFRELNYSMGMEMAYSNLARWAAIHGD
                     FQESEKYVALAQASQKSGTIRIQSGFLNMGLGIGARFQGRYDVAMRHFEEGLRIFKHI
                     GHKGMVAAMTSEVAHTKRALGNYAEAKQTYYETIKVFQDYGNRPSVAHQLECFAMIAV
                     AEEEPQRAAKLFGAAEAIREMTGHKPTDEEQAEEAQFITRLHAMLSAKYPQGDTEFNA
                     LWAEGKSMTMEQAIQLALHGHE"
     gene            4322..4819
                     /locus_tag="PLT08_03505"
     CDS             4322..4819
                     /locus_tag="PLT08_03505"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024812.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNE03558.1"
                     /translation="MNITIRRVEPTDYEAMQKLFDGPQVVWGTLQLPFPSVEMWRKRL
                     AEPPEGAFGLVACVENEIVGQLTIHTNPSRPRRRHAGGIGMAVRDDFQGKGVGTAILR
                     AATDMADNWLNILRLELEVYTDNEPAVRLYKKCGFEIEGTLKRHSFRAGQYVDVYTMA
                     RFKPD"
     gene            4844..5365
                     /locus_tag="PLT08_03510"
     CDS             4844..5365
                     /locus_tag="PLT08_03510"
                     /inference="COORDINATES: protein motif:HMM:NF024698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNE03559.1"
                     /translation="MGIILETNRLLLRHQVLSDLDDLWALYCDPAITKYIPDAPRSRE
                     EAKEELEWHMNGHPRHPELGLWATIHKQTGKFIGRCGLLPWEIDGQSEVEVAYTIAVE
                     YQGQGLGSEAANAILNHGFETLNLSRLICLIDPENIPSQKVAERMGMKFEKESSDEYG
                     TFRVYSIKKEADA"
     gene            complement(5391..>6067)
                     /locus_tag="PLT08_03515"
     CDS             complement(5391..>6067)
                     /locus_tag="PLT08_03515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE03560.1"
                     /translation="HYNLELEEVLIGTPSSPAGDKQIETILTQLRQRQIAEEQVETYS
                     QQEKAAVKERELREAESRAAMQGKMTEAELSINIQSNQGKAEYERSIQQAQQIRALAE
                     AEAEKVARVGIAQALATEEQVRAYGGPQFQVTQQVLNRFADAIEKSKVDVVPRVVVGG
                     SSEKGGSQTSSIMEALLTMLLSDRFGALASEAGTTQRSPDVEAMREKILKDMQKKDNK
                     GENKKA"
BASE COUNT         1496 a         1566 c         1714 g         1291 t
ORIGIN      
        1 atgtcggtgg atgcacgccg aatggaaaca tcggggtcgt ctgcgccttg cagtactaca
       61 gcaacggctg cggcatctgt gtggaatccg ccgaagggct gttcttcact gaaaaagatc
      121 tgcggctgcc cctcgcgcag ggcatcgaca agtgaaagcc cataggcttt actggcgcgc
      181 cagattacat aaaccaaggc tgcggcagaa aaaagggcga cgatatttag ttgctgcgaa
      241 ttcaaggcgc ggtcgccaag gaataaaagc aagcccgcag cgaaaacacc caactgttcg
      301 ggcgcgccgc caatgaaggt gcgcacctgg tcgcgttgat tggcgggcac cacattgaaa
      361 agagtttgcc atgcggttcc tgcaatgcct tgcatccaga ccatttgcaa aaagcgcaca
      421 gacaccaagg cggggaaggg cgcaaaaaag gacaatacgc caaaccccag caaataaatg
      481 acggggaaga gcagcaacat gggcatgatg ccaaagcggg caaacagacg attggcaagg
      541 aataacgaaa cgatcaaggc gatgatcgtg ttgatgcttt ggaatagacc gaggaaccct
      601 gcaagttggt ctgcgctggg gaaccgggcg ctggcgccgc gtgagaattg caggtccagg
      661 aagaagtagc acagtgaaaa taaaaaggcg ctgtacgcta cccattgcat gatggatgaa
      721 cggcgcacga attgatatcc gcgctgcatt tccgtgagga tgtttgcatt accggcgcgg
      781 gaggatgttt cgggcacagc accgccaacc agcgtgcggc caaggtaaaa gcagaggagg
      841 aatacgccag cccatagcag tacgagagtt tcagaatgaa gcctgcccgc caggggtggg
      901 gttaaaaaac cgccgagcac agcgcctaaa ataccgcccg cgctaaacaa ggggaacagg
      961 cgttttgcct ggcgcgtatc acaagccgcg ccagccagcc cccagaccca tactccctgt
     1021 aaacttccaa tgacgttcat acccagccac atgacggcga agatccattt aatccccaga
     1081 cccaatgtta tccatcccgc aagcaatacc atgccaagga tgacaggcag gtaggtgaag
     1141 agatgttttt ttgaaaaccg tcccatgatg atggtgattc ccatcgaagt catgaaggtc
     1201 aacgtcccca aaagcatata catgaccggc agagactcca caccgaaacg gttgaagaac
     1261 aaagccgaga cggcattccc tccaagcgca ctgccggcgg aggtgagcag catcaacccg
     1321 accagcaatg tggacatgcg ttcttcgcct gggttgattt tgaagatctc taaaattcgt
     1381 ttcatacaaa acctgttccg ttcgaggcac gggcgtatca gaggtatttt actttcaaat
     1441 gtggattaga atactccaaa taagtcagac cggttcgttt tggagattgg tcatgagcgg
     1501 attgcactca ggaacggtca cattcctatt caccgatata gaggggtcta caaggctttg
     1561 ggaagagcat cccgaagcga tgaaaaccgc gctggcgaaa catgattcgg ttctaaggga
     1621 atccattgaa tcaagtcacg ggcaaatcat caaaacaaca ggagatggcg tccatgcggt
     1681 cttcgaaact gccatcgact ccatccgcgc atccattgcc gcacaaagcg ggtttcagac
     1741 cccggagttc ttaaataaat ccggggtctt gttgcgcgtc cgcatgggca ttcacaccgg
     1801 cgaagccgaa ttgcgcgacg gcgattatta tggcagcgta ctgaaccgtg cagcccggtt
     1861 gatgtccatg gcgcatggcg gacaaatttt gatctcgaac gccacgtcgg aacttatccg
     1921 cgatggattg cctgcgaatg taaccctgcg ggatctgggc gaacatcgcc tcagggactt
     1981 gacccgaccg gaacacattt ttcaactggt tcaccctgat cttcccggtg aattcccgcc
     2041 tcttaaaaca ttggatgctt ttccaaataa cctgcccgta caactgactt cattcatcgg
     2101 gcgcgaaaaa gagattgccg aaataaaatc tgcgcttggt tctgcaaggc tcgttacgct
     2161 cactggctca ggcggcacgg gcaagaccag actctcacag gaagtcggta tgcaactgtt
     2221 gacaaacttc cagcatggcg tgtggctggt ggaactcgcg ccgttgagcg atgagtcgca
     2281 gatcatccct gcactggcgc aggtgtttgg attgcaggaa ttgccgttca atccgctggc
     2341 gaaactggtg gaagattacc tgcgcgacaa aaaacttctg ctcattctcg ataactgcga
     2401 gcaccttatc gcgggttgcg cgcggcttgc ggaccatctg ctccatcaat gtgctggact
     2461 caagatttta gcgagcagtc gcgaggcgtt gggcatcgcg ggcgaagtgg cgtatcgcac
     2521 accttcgtta tcttcccctg ctctcggtgg cttccccgcc gccgaggacg gcggcttgag
     2581 caaaaaaata ttggaatacg aatcgaccag ccttttcgtg gaccgcgccc gcgccgccaa
     2641 ctcgaagttt gctctcaccg attcaaacgc ttcttccatc gcccagatct gtcaccgcct
     2701 cgacggcatt cctttggcga tagagcttgc cgccgcacgg acgaaactcc tttccgcgga
     2761 acagatcgcc acacggctcg atgacatgtt ccgactgctt gtcggcggaa gtcgcaccgc
     2821 cctaccgcgt caacagactc tgcgcgcgtt gatcgattgg agttacgact tactatcaga
     2881 cgaagaaaaa cggctcttgc aattcgcctc ggtcttcgtt ggcgggtgga cgttggaggc
     2941 gttggaattc gtcgcggaag aaccgtctac gctggagtta ttggagcaac tcgtcaacaa
     3001 gtcgttggtg gtgacggaag aacacaaagg cgagatgcgt tacttcctgt tggagacgat
     3061 tcggcaatac gggagggaaa agctgttcga agcgaagcag gcttcggcgg cgcgtgatcg
     3121 gcatttcatc tactataaaa atttaacgaa tggcttatgg gatgtcactt atttcgcaag
     3181 cgaagggcag acgcaaagat tgatctccca gcaagcagaa gtcgaaaact tgcgggctgc
     3241 gttcgaatgg ggtttacaga atgatgcgca agacgcactg gaacttgctg cgaacatagc
     3301 aatgagcatg agcatgatgg gggggcaaag cgaaggaacg aatatgctca aaacagcaat
     3361 ggagaagttt cgcgccctgc cgcccgccgc aggtggcgat gccgaacgtg ttcgaaaaga
     3421 aatatatgcc catgggtgtt ttagcctggg aatgttgctg cagggtacga atgaaatcac
     3481 ttactcgcgt aataccttgc aggaagccat tgccatcgca cgcgaactcg gtgataaaca
     3541 catgttaggc atagcgctgg aaatgtacgc caacgcctcc gccatgatca aagcagatga
     3601 taccgtcacc gccgcacagg aggggctaga gatttttaga gagctcaact acagcatggg
     3661 gatggagatg gcttatagca acctggcgcg ttgggcagcg atccatggcg attttcaaga
     3721 gagcgagaag tatgtggcgc tggctcaagc tagccagaaa agcggaacca tacggattca
     3781 atctggtttt ctcaatatgg gtctggggat cggcgcgcga tttcagggtc gctacgatgt
     3841 tgccatgcgc cactttgaag aagggctcag gatattcaag cacatcgggc ataaaggcat
     3901 ggttgcggca atgacttccg aagtcgcgca tacaaaacgc gccttgggca attacgccga
     3961 agccaagcaa acctattacg aaactatcaa ggtttttcaa gactacggca atcgtccgtc
     4021 tgtggcgcat caactggaat gttttgcgat gatcgcagtt gcggaggaag agccgcaacg
     4081 cgccgcaaaa ctattcggcg cagcagaggc gattcgtgag atgacaggtc ataaacccac
     4141 cgatgaagag caagcggaag aagcacagtt cataacccga cttcatgcga tgctgtctgc
     4201 gaagtacccg cagggtgata cggagttcaa tgcgctctgg gcggagggta agtccatgac
     4261 gatggagcag gcgatccagt tggcactgca cggtcacgaa taatttggaa aggaaaagag
     4321 aatgaacatt acgatccgcc gcgtagaacc aactgattat gaagccatgc aaaaactatt
     4381 tgacggacca caggtggtgt ggggtacgct gcagttgcca tttccctcgg tggagatgtg
     4441 gcgcaagcgt ttagccgagc cgcccgaggg ggcattcggt ttggtggcgt gcgtggagaa
     4501 tgagattgtc gggcagctga ccattcacac gaaccccagc cgcccgcgcc gccgccatgc
     4561 tgggggcatt ggaatggcgg tgcgcgatga ttttcagggc aagggtgttg gcactgcgat
     4621 cctccgcgcc gctactgaca tggcggacaa ttggttgaac atcctccggc tggaattgga
     4681 ggtatacaca gacaatgagc cagccgtgcg gctatacaaa aaatgtggat ttgaaatcga
     4741 ggggacgctc aaacgccata gtttccgcgc cgggcaatac gtggacgtat acaccatggc
     4801 acgtttcaag ccggattgat cagcgcgctc cttttacttg tatatgggaa tcatcctcga
     4861 aacaaatcgg cttttgctcc gtcatcaagt cctttcggat ctggatgacc tgtgggcgtt
     4921 atactgcgac cccgcgatca ccaaatacat tcccgatgcg ccgcgttcgc gcgaagaagc
     4981 caaagaagaa ttggaatggc atatgaatgg gcatcccaga catcccgagt tgggattatg
     5041 ggcaacgatc cataaacaga cagggaaatt tattggccgc tgcggactgc tgccgtggga
     5101 gatcgatggg cagagtgagg tcgaggtggc gtatacgatc gcggtggaat atcaaggtca
     5161 gggcttggga agcgaagcgg cgaacgccat cctgaaccat gggtttgaga ctttgaatct
     5221 ttcacgcttg atctgtttga tcgacccgga gaatatccct tctcaaaagg tggcggaaag
     5281 gatggggatg aagtttgaaa aagaaagcag tgacgaatac ggaacattcc gggtgtactc
     5341 gataaaaaaa gaggctgatg cttgaagcac cagcctcttt ttgataaaga tcaggctttc
     5401 ttgttttcgc ctttattatc cttcttctgc atatctttca atatcttctc acgcatggct
     5461 tccacatcgg gggagcgttg ggttgttccg gcttcgcttg ccagcgcgcc aaagcggtcg
     5521 gagagcagca tggttagcag ggcctccatg atgctgctgg tctggctgcc gcccttctcg
     5581 ctgctgccgc ccaccaccac acgcggaacc acgtccacct tggatttttc aatggcatcc
     5641 gcaaagcggt taagtacctg ctgggtcacc tggaactgcg gcccgccata cgcgcgaacc
     5701 tgctcctcgg tggcgagcgc ctgcgcgata ccaacgcggg cgaccttttc ggcttccgct
     5761 tctgccagcg cacggatctg ctgggcctgt tggatcgagc gttcgtactc cgccttaccc
     5821 tggttcgact ggatgttgat gcttaactcc gcctcagtca tcttgccctg catcgcggcg
     5881 cgggattcag cttcacggag ttcgcgctcc ttgacggctg ccttttcctg ttgagaatag
     5941 gtttccactt gctcttccgc gatctgacgc tggcgcaatt gggtcaggat ggtttcgatc
     6001 tgtttgtcac cggcaggcga cgagggggta ccgatcagca cttcctccag ctcgaggttg
     6061 tagtgcg
//
LOCUS       DAOQJE010000087         6064 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_7374_length_6064_cov_5.577800, whole genome shotgun sequence.
ACCESSION   DAOQJE010000087 DAOQJE010000000
VERSION     DAOQJE010000087.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 6064)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6064)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6064
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7374_length_6064_cov_5.577800"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..802)
                     /locus_tag="PLT08_03520"
     CDS             complement(<1..802)
                     /locus_tag="PLT08_03520"
                     /inference="COORDINATES: protein motif:HMM:NF019581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HNE03561.1"
                     /translation="MKLLYNANIHTLDSANPHASAILIAGGRIIAVGSKDQLESLAHG
                     KVEKQDMKGKTILPGLTDAHIHMQFYSLGLSKVDCETKTKEECLRRVGERARTLKPGE
                     WVLGHGWQQNDWDGNFPKAAELDAVSPNNPVYLTAKSLHAAWANTSALKLANITDETP
                     DPKDGAVQRDANGKATGIMIETAMALVNAAVPEPTLGELEAAFEKAQSVLWKMGITGV
                     HDFDRRQSFMALQSLRAKGKLKLRVCKNIPVESVEQANDLGLRTGFGDE"
     gene            complement(875..1747)
                     /locus_tag="PLT08_03525"
     CDS             complement(875..1747)
                     /locus_tag="PLT08_03525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HNE03562.1"
                     /translation="MAQRKGLGKGLDALIPGGKSSTPAVTSSKTSGEGGVQQVPVEAI
                     KRNPRQPRVHFKEEDLNDLAASIREHGVIQPLIVSPKGDGTFILIAGERRWQASQRAG
                     LTKVPVVTRQANNQELLEIALIENVQRADLNAMEEAEAYHQLAEDFGLSHEMIAKRVG
                     KSRVAVTNTMRLTNLADAVKQALVDNTITEGHARALLGLSTQKAQTSALQTIINLSLS
                     VRQSEELIRKLTGVKPVKVKKATVSADVLDVEKRLQRSLGTKVALKHGKKGGTVTIYY
                     YSDEELDALIKKMS"
     gene            complement(1814..2584)
                     /locus_tag="PLT08_03530"
     CDS             complement(1814..2584)
                     /locus_tag="PLT08_03530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012096464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HNE03563.1"
                     /translation="MTKIYTLVNQKGGVGKTTSTINLAAYLAKLGQRVLVVDLDPQAN
                     ATSCLGVDKLLVEGSTYDALLGEDDIFPFILLNERLQLSLLPSSPSLAGAEVELVDEL
                     ARESRLRKALLKVASRYDYVLIDCPPSLGLLTVNGLMAAIDGVVVPVQCEYLALEGLG
                     QLTETIERVRAALFPDLKVRGVILTMFDNRTNLSADVVREVNKHFPNQVFKSIIPRNI
                     RLAEAPSYGLPISEYAPNSPGADAYETLAKELLKGDKN"
     gene            complement(2581..3423)
                     /locus_tag="PLT08_03535"
     CDS             complement(2581..3423)
                     /locus_tag="PLT08_03535"
                     /inference="COORDINATES: protein motif:HMM:NF012515.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HNE03564.1"
                     /translation="MSDLRPVDYLLIGHVAVDLTPSGKQLGGTVSYAALTAKAMGLRV
                     GIVTSAGEDAPLSLLDGVQILNLPTEQSTIFENVKTEHGRKQTLHHQATKISFEHVPQ
                     IWRDAAIVHLAPIAQEVDPALVGQFPASWVGVTPQGWMRGWDEKGSVFATAWENSEQV
                     LGQVGGVVMSLEDINHDLELGEWLAHHARLLCLTEGEEGAVLHWHGDRRRYRPIKVEE
                     VDATGAGDIFAAGFFSRLHQTKDPWEAARFATHLAAYSVTRLGLQGIPTHDEIEHCKM
                     EVLS"
     gene            complement(3534..4397)
                     /gene="jag"
                     /locus_tag="PLT08_03540"
     CDS             complement(3534..4397)
                     /gene="jag"
                     /locus_tag="PLT08_03540"
                     /inference="COORDINATES: protein motif:HMM:NF041568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_process: GO:0051510 - regulation of unidimensional cell
                     growth [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding cell elongation regulator Jag/EloR"
                     /protein_id="HNE03565.1"
                     /translation="MSERTTLEIIAPTVEEAIQQGLDQLGLTADAVSVEVLDSGSKGL
                     FGLGGRQVRVRISVNPPPMPEIPAARPEPKPAPAKKAESKPKAKEPKEPKPRAEKKQA
                     EPKTEAKPEPKPEPKPQKPAPQRSTINVENDAVLNTAEEVVSKLIYHMGMTAQVSAHY
                     DESDSADNRTIQVDVRGEDLSALIGRRAETLASFQHVASLIVGKQTQQWVQVIIDVEG
                     YRARREKQIRQLANRMADQVTKTGRKVTMEPMPSSERRVVHIELRGHPAVKTESTGEE
                     PYRKVVILPKE"
     gene            complement(4407..5225)
                     /locus_tag="PLT08_03545"
     CDS             complement(4407..5225)
                     /locus_tag="PLT08_03545"
                     /inference="COORDINATES: protein motif:HMM:TIGR03592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YidC/Oxa1 family membrane protein insertase"
                     /protein_id="HNE03566.1"
                     /translation="MWESFIIQPMVNLLLWIYDILGHGPQMFGLAIILFTIVIRLITW
                     PLNASQVKGAQAMQELNSDKEWQDIQKKYAKDREKLAQEQMRIYKERGINPFASCLPT
                     LIQFPIIIGLYQSIIRAMAATPLDMLNLARTIYPFQHVSNIIPLNSTFLWMNLGRPEG
                     IPLPFDVSFLPNGFPLLALVVAITTYVQTKLTMPTTPPNPNDQASAQTAAMSSSMSIY
                     MPLMLGWFALTFASGISIYFITSNTLGIIQYAVTGRANWSNLLPGGNKSTPTKK"
     gene            complement(5238..>6064)
                     /locus_tag="PLT08_03550"
     CDS             complement(5238..>6064)
                     /locus_tag="PLT08_03550"
                     /inference="COORDINATES: protein motif:HMM:NF024753.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HNE03567.1"
                     /translation="QLLVGSEGNNHELVSINPETGKDNWAKPFSVAKGKWVASPLVFN
                     DIVYAPNSDGFLYILDLNGETSADPIELGGSLWSTPVTDGTYIYVTSLDHHLHIVDPA
                     SNSIVSTIDLGGASPSSPVVGEGGVYVGSFASNIEFVTSNGDHEVLATATNWIWGTPA
                     LDNGTLYYADLDGVLYSLDVNSGKQNWTDVKPDGPVIANPVVVGDAIYFVTEAGTFLA
                     LDRDAKTIWEKEVGGKIYTTPVVSGDMVLVAPYKAEFALAAYDAEGKQAWTFTPEK"
BASE COUNT         1432 a         1642 c         1468 g         1522 t
ORIGIN      
        1 tctcatcacc gaagccggtg cgcaagccca ggtcgttcgc ctgctccacg ctttcaacgg
       61 ggatgttttt gcacacgcgt aattttaatt tgccttttgc tcgcaaagat tgcaaagcca
      121 taaacgattg acggcgatca aaatcgtgga cgcccgtgat acccatcttc cataacacgg
      181 actgtgcctt ctcaaacgcc gcctcaagct cgccgagagt tggttctggc accgcagcat
      241 tgaccaacgc catcgccgtt tcaatcatga tgcccgtagc tttcccgttc gcatcgcgct
      301 gaacggctcc atctttcgga tcgggtgttt catccgtgat attcgccaac ttcaatgcgg
      361 acgtattcgc ccatgccgca tgcaatgact ttgcggtgag atagacagga ttattcggcg
      421 agaccgcatc tagctccgcc gcttttggga agttgccatc ccaatcattc tgttgccagc
      481 cgtgacccag cacccactcg cccggcttga gagtccgcgc acgttccccc acccggcgta
      541 gacattcttc cttcgtcttg gtctcgcaat ccactttgga aagcccaagc gaataaaact
      601 gcatatgaat gtgcgcgtcg gtcagacccg gcaagatagt cttacctttc atgtcttgct
      661 tttccacctt gccatgtgca aggctttcaa gctggtcttt gctacctaca gcaataatcc
      721 gcccacctgc gatgagaatc gcagaagcat gcggattagc agaatcaaga gtgtgaatgt
      781 ttgcgttata gagaagtttc atatttttgt aggtgcaggt ctcctgccca aagctttaca
      841 caggaataag gcgcggaaac cgcgcctcta cgaattacga catcttctta atcaatgcat
      901 ccaactcttc atcggaataa taataaatcg tcaccgtgcc gccctttttg ccatgtttga
      961 gcgcaacctt cgtgcccaaa ctgcgctgga ggcgtttctc tacatccaac acatcggcgc
     1021 tgaccgttgc cttctttacc ttcacaggtt ttacacccgt caacttacgg atcaattctt
     1081 cagactgacg gacgctcaac gacaaattga taatggtttg cagtgcagag gtttgcgcct
     1141 tctgagtgga cagccccaat aaagcacgag cgtgaccttc ggtgatggta ttgtccacca
     1201 aggcttgttt gaccgcgtct gcgagattgg taaggcgcat agtattggtt actgccacgc
     1261 ggctcttccc cacacgtttc gcgatcattt catgcgaaag cccgaaatcc tccgcaagct
     1321 gatgataggc ttcggcttct tccatggcgt tcaagtctgc gcgttggacg ttttcgatca
     1381 acgcaatttc aagtaattcc tggttattcg cctggcgcgt taccacaggg actttcgtca
     1441 acccagcccg ctgcgaagcc tgccaacgac gttctcccgc aataaggatg aaagtgccat
     1501 ctcccttcgg agagacgatc aacggttgga tgactccatg ctcacgaatg gatgccgcca
     1561 agtcgttcag gtcttcctct ttaaaatgca cacgcggttg ccgagggttg cgcttaattg
     1621 cctccaccgg cacctgttgt acaccgcctt ctccacttgt tttgctgctc gtcaccgccg
     1681 gagtcgaaga ctttccgccc ggaatgagcg catccaaacc tttgcctaaa ccttttcgtt
     1741 gagccatgtt ctctaccttt tcgaccacaa agtattgaag ttttccttcg tgtctctttg
     1801 tgacacttcg tggttaattt ttatcgcctt tcaacaattc tttcgccaat gtttcgtagg
     1861 cgtctgcacc gggagaattg ggtgcgtatt ctgagatagg caatccatag gaaggtgctt
     1921 ctgccaaacg aatgttacgc ggaataatgc tcttaaacac ctgattgggg aaatgtttat
     1981 tcacttcgcg tacgacatcg gcagaaagat tggtgcggtt gtcgaacatt gtcagaatca
     2041 cgccgcgcac tttgaggtcc gggaaaagag ccgcgcgcac gcgttcaatc gtctcggtca
     2101 actgacctaa tccttcaagc gcaagatatt cgcattgcac cggtaccacc acaccatcaa
     2161 ttgctgccat caagccattc acggtcagca gtcccaaaga aggcgggcaa tcaatcaaca
     2221 cataatcata acggcttgcc accttcaaca atgccttgcg caaacgagac tcgcgtgcaa
     2281 gctcatccac cagttctact tcggcgccag ccaaagaagg cgaagaaggc aacagcgaca
     2341 actgcaaccg ctcattcaat aagataaacg gaaagatatc atcctcgccg agcagcgcat
     2401 cataagtact gccttctacg agaagtttat ccacgcccaa acaagaggtt gcgtttgcct
     2461 gcgggtcgag gtctaccacc aacacacgtt gccccagctt cgccaaatac gccgctagat
     2521 tgatcgtgga tgtggtcttc cccacaccgc ctttttgatt caccagagta tagatcttcg
     2581 tcaagacaaa acctccatct tgcaatgttc aatttcatca tgagtgggaa tgccctgcaa
     2641 acccaaacgt gttacagaat atgccgccaa atgagtcgca aaacgtgctg cttcccacgg
     2701 gtcttttgtt tgatgtaacc gcgaaaaaaa tccagccgca aaaatatcgc ctgcccccgt
     2761 cgcatccacc tcttccactt tgatgggacg atatcgtctg cggtcgccat gccaatgcaa
     2821 caccgcgccc tcttcccctt ccgtcaaaca cagcagacgg gcgtgatgtg ccagccattc
     2881 accgagttcc aaatcatggt tgatatcctc aagcgacatc accacgcctc ccacctgacc
     2941 gagaacctgt tcgctgtttt cccaagccgt agcgaacacc gaaccttttt cgtcccagcc
     3001 acgcatccaa ccctgcggcg tgactcccac ccacgaagct gggaattgcc ccaccaaagc
     3061 cgggtccact tcctgtgcaa tcggcgcgag gtggacaatc gccgcatctc gccagatctg
     3121 tgggacatgc tcaaacgaaa tcttcgtcgc ttgatgatgc aaggtttgtt tgcgtccgtg
     3181 ctcggtttta acgttctcaa aaatcgtgct ttgttcggtt gggaggttta atatctgaac
     3241 cccatccaaa aggcttaagg gagcatcctc ccccgctgag gtgacaatac caacccgcaa
     3301 gcccatcgcc tttgccgtca acgccgcgta tgaaaccgtc ccaccgagtt gtttgcccga
     3361 tggagttaaa tcgacagcca catgaccaat aagaagatag tccactgggc gaaggtcaga
     3421 cataggtgaa ttataccgta gtgaaatctt tcctctttct tctttcttgc aagaagaaac
     3481 aaaaagaccg tagattttca cctacggtct tttatctatc agtaaatgtc gaactattct
     3541 ttcggcagaa tgaccacttt gcgatagggt tcttcgccgg tgctctcggt cttcacggca
     3601 ggatgtccac gcaattcgat gtgcacgaca cggcgctcac tagaaggcat cggttccatg
     3661 gtcaccttgc gtccggtctt ggtcacctgg tcagccatgc ggttggcaag ctggcgaatc
     3721 tgcttttcac ggcgagcgcg gtagccttcc acgtcgatga tgacctgtac ccattgctgg
     3781 gtctgcttgc caacgatcaa cgatgcgacg tgttggaagg aagccaaggt ttcggcgcgg
     3841 cgtccgatca gggcgctaag gtcttcacca cggacgtcca cttgaatggt gcggttatcg
     3901 gcagagtctg attcatcgta atgagcagag acctgtgcag tcatgcccat gtgatagatc
     3961 aacttggata ccacttcttc cgccgtattc aacaccgcgt cattctcaac attaatcgtt
     4021 gagcgttgtg gagcaggctt ttgcggcttc ggttcaggtt tcggttcagg cttggcttct
     4081 gttttgggtt ctgcctgttt cttttcagct cgtggcttgg gctcttttgg ctctttcgct
     4141 ttcggcttgg actcagcttt tttcgctgga gcaggcttcg gctcaggtcg agccgcgggg
     4201 atttccggca tgggcggagg gttcacagaa atgcgaacac gcacttgacg tccaccgagt
     4261 ccaaataatc ctttggagcc ggagtctaga acttctacag aaaccgcatc cgccgtgagc
     4321 ccgagttggt cgagcccttg ctggatggct tcttcaacag ttggagcgat aatttctaac
     4381 gtagtccgct cactcatggt gcctccctat ttctttgtag gtgtagactt gtttccaccg
     4441 ggcaacaaat tgctccagtt ggctcgaccc gttaccgcat actgaatgat gcccaatgta
     4501 ttgctggtaa taaagtagat cgaaatgcct gaggcaaatg taagcgcaaa ccaacccaac
     4561 atgagcggca tataaatgga catgctgctg ctcatggcag cagtttgtgc agaagcctgg
     4621 tcattggggt tgggcggtgt agtaggcatg gtcagtttgg tttgaacata ggtagtaatt
     4681 gccacaacca acgccaagag cgggaaaccg ttcgggagga aggacacatc gaacggaaga
     4741 ggaatccctt cggggcgacc gaggttcatc cacaagaatg tactgttaag cgggatgatg
     4801 ttcgaaacat gctggaaagg gtaaatggtg cgcgccaaat tgagcatgtc gagcggggtg
     4861 gccgccatag cgcgaatgat gctctgatac aagccgatga tgatcggaaa ttggatcaag
     4921 gtcggtaaac aggaagcgaa aggattaatg ccgcgttctt tgtaaatacg catttgttcc
     4981 tgcgccagct tttcgcggtc tttggcatac ttcttttgaa tgtcttgcca ttccttgtcg
     5041 ctgtttaatt cctgcatggc ttgggcgccc tttacctgtg aggcattgag gggccaagta
     5101 atgagcctga tcacgatggt gaataaaatg atggcaagac caaacatttg cggtccatga
     5161 cctaaaatgt catagatcca caaaagcagg ttaaccatcg gttggatgat aaatgattcc
     5221 cacatgggct aggttcctta tttctcgggg gtaaacgtcc aggcttgttt gccttcagcg
     5281 tcataagccg ctaatgcaaa ttctgcctta tacggcgcaa cgaggaccat gtcgccggaa
     5341 acaacaggag tggtatagat tttgccgccc acttcttttt cccaaatggt ctttgcgtca
     5401 cgatccaaag cgaggaaagt acccgcctca gttacgaaat aaatggcatc gccaacgact
     5461 actgggttcg ctatgacagg accatctggc ttgacatcgg tccagttctg tttgccagaa
     5521 ttcacatcga gggagtaaag aacaccgtcg aggtcggcat agtacaaagt gccgttatcg
     5581 agtgcgggag ttccccagat ccaattcgta gcagttgcca aaacttcatg gtcgccattg
     5641 gaagtgacaa actcaatgtt cgaagcaaat gagcctacat atactccacc ctcacccaca
     5701 acggggctgc tgggagaagc accgccaagg tcaatggtgc tgacgatgga attgcttgca
     5761 ggatccacaa tgtgaaggtg atgatctaac gaagtgacgt aaatgtaagt accatccgtc
     5821 acgggcgtgg accaaagtga gccgcccaat tcgatggggt cggcagaagt ctcaccgttc
     5881 aagtcgagga tgtagagaaa gccgtcggaa ttgggagcgt agacgatatc gttgaaaaca
     5941 agcggagatg ccacccactt ccctttagca acagagaagg gttttgccca gttatctttg
     6001 ccagtttcgg ggttgatgct caccagttcg tgattgtttc cttcacttcc caccaacaat
     6061 tgac
//
LOCUS       DAOQJE010000088         6025 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_7443_length_6025_cov_5.151089, whole genome shotgun sequence.
ACCESSION   DAOQJE010000088 DAOQJE010000000
VERSION     DAOQJE010000088.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 6025)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6025)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6025
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7443_length_6025_cov_5.151089"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1074)
                     /locus_tag="PLT08_03555"
     CDS             complement(<1..1074)
                     /locus_tag="PLT08_03555"
                     /inference="COORDINATES: protein motif:HMM:NF014759.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-3' exonuclease H3TH domain-containing
                     protein"
                     /protein_id="HNE03568.1"
                     /translation="MPPTLYLIDGHALAYRMYFALTAGGGSQRWQTSKGEPTAGIYGF
                     ARELVRVLEQEKPEYLAVAFDVGKTFRDQIFPEYKATREKMPDDLRSQIARIREMVDA
                     FNIPRLEMDGYEADDVLGSVAKLAAEKGLGVKIITGDRDLLQLVNERTAVYLAGDDQT
                     YITDADVIKKLGVRPNQVVDYKAIVGDTSDNIPGVKGVGEKTAVALLEKFNTLDAIYA
                     NLDQVENRWRGKFETNKETAYMSRDLAQIETNLKIKFDFEHAKVAPFDGSKLEAFFKE
                     MEFRTLVSKVPAISGGVASASAEIKAPTVKTGKTKAGQMTMFAEEAQPVAVAIKPASI
                     EVVIVDSAEKLKSLANELEKAKVI"
     gene            complement(1181..2851)
                     /locus_tag="PLT08_03560"
     CDS             complement(1181..2851)
                     /locus_tag="PLT08_03560"
                     /inference="COORDINATES: protein motif:HMM:NF013921.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - transposition, DNA-mediated
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HNE03569.1"
                     /translation="MPADLMVVSPSSSLGEAIRRSLEDTKFYRVTTVNNKAAAIVKAN
                     EIGMPMAMLDLALGEEWVAEIGASLRTIRPSINLVILCEEAGSPPAFDSLRPWIMVRK
                     PFKISDFMNAISQPQSAQPAKSGAPAPAMPMSSTAQFMPWLNDPNKAAQHLTRLTLES
                     SAQAALITKKNDLWAYAGGLSQNAAKEVAQTVTRNWDGQKGSDLLRFIRLESTKAEHM
                     LYATRLTSDAVLALVFDAETPFSTIRSQASQLLNDFGTEKAEPKNQPAVSYAVSEDNN
                     KDEDLNIPPISNILADIPAPNPEPLTTRDFNLPNKKPAQPQPQEAFDPNQTRVSDALS
                     NASVFSRDPSPAVRRDSVPQPMAQTQKSEAHHDTQIDFDEVQVTAPSQPKARPETPVA
                     RPRPGENDATRESPSTEMARKLTAEPISAGLYHLTYACLLVPRFSSHYLTGDLSDRLG
                     EWLPNICIAFGWRLEFLAVRPEYLQWVVNVQPNTSPGHLMRIMRQQTSEKIFGDFPRM
                     KRENPSGDFWAPGYLIMGGSQPHPPQLVRDYIRQTRQRQGLEDLPPMK"
     gene            complement(2937..3500)
                     /locus_tag="PLT08_03565"
     CDS             complement(2937..3500)
                     /locus_tag="PLT08_03565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE03570.1"
                     /translation="MLKPTDLILVCLMPTPRDMEIARLLGWYRIPLKSAPKVVSVDYL
                     AFYQPASFGERGGQVEYIAQVRGHELTTRGELLKDEADHPRAKEEYFKLQLGNLEKLK
                     EPVRTDKWKRLIFLYSTGEYLLNAKTLNDLVVEGEERNMLWKNLRERAENDQLYQVDL
                     PEADIPPEVLMALLGIKDLAGGYDAGQ"
     gene            3513..4520
                     /locus_tag="PLT08_03570"
     CDS             3513..4520
                     /locus_tag="PLT08_03570"
                     /inference="COORDINATES: protein motif:HMM:NF012907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 18 protein"
                     /protein_id="HNE03571.1"
                     /translation="MFTMRLSFLLLSCSLLVACGGGATPLPAPEVDSTPIPSSTPQPT
                     ATSAPVPNFPVIGYLPDYRELNPAWASSLTDIIYFSAEPRADGSLDTSRLTEETWQAL
                     ASLKDENGIRVHISIGGWGRDGAFVEMSADPETRKTFIANLLSFLQEHQLDGADFDWE
                     FPQNDTEFEQYIALLTETKAALSPHGLLVSVALPAESSFPLGEFAVVDRVHLMSYDRG
                     ERHSTFEQSVADVEDFLEAGIPPEKLILGMPFYGRGMSSPSDTYSYEQIRSTYAPAPD
                     VDEVGDIYFNGLNTIQHKVCYGIAQNLGGFMVWELGLDTVDESSLLQQIYRLAQGAES
                     C"
     gene            4514..5140
                     /locus_tag="PLT08_03575"
     CDS             4514..5140
                     /locus_tag="PLT08_03575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE03572.1"
                     /translation="MLIHPSPYERITALTDIFLGILAAWGAAQLFAFADFRHQIWAWT
                     FCLLAIASFLGAIAHGFVMSTKTNDRLWMPINVALGLTLGLFVVGALFDLSGESVARQ
                     ALPVMIALGFGFFLITILKPGTFMTFIAYEALAMLFALGIYGYLFFTSNLLGVGWMVL
                     GVLVTIIAAVVQATGNAGKSIFWYFDNNGVFHLIQMIGMVLLYFGLNT"
     gene            complement(5171..>6025)
                     /locus_tag="PLT08_03580"
     CDS             complement(5171..>6025)
                     /locus_tag="PLT08_03580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-5-phosphate-dependent protein subunit
                     beta"
                     /protein_id="HNE03573.1"
                     /translation="DLMIFDQFQEFGNYLWHYTVTGHAMEEVFRQVAGKNGRFRGMAS
                     ATGSAGTIASGDYMKQLFPDSKIVASEALQCPTLLENGFGAHRIEGIGDKHVPWVHNT
                     KNTDVVTAIDDNAVVNISRLFNEENGKAYLVKMGVSEDVVNQLDLLGFSGISNVLSAI
                     KTAKYYEMGEDDIMITVLTDSMELYRSRLHEMHQEFGEYTEANAAADFARYLHGQSTD
                     NMLELRYPDRRRVHNLKYYTWVEQQGKTYDEIQAQWYQPDYWTSVQSQADEIDELIVE
                     FNKEVGLS"
BASE COUNT         1306 a         1494 c         1594 g         1631 t
ORIGIN      
        1 aatgaccttg gctttctcca actcgttggc gaggctcttt aatttctcgg cgctatccac
       61 aatcacaact tcaatgcttg ccggtttgat cgccaccgcc acgggttgcg cctcttctgc
      121 gaacatggtc atctgcccgg ctttggtttt gccggttttc acagtggggg ctttgatctc
      181 tgctgaggca gaagccacac cgccgctaat ggcaggcact ttgcttacca gagttctgaa
      241 ttccatttct ttgaagaaag cttccaactt cgagccgtcg aagggtgcca ccttggcatg
      301 ttcaaaatcg aatttgatct tgaggttcgt ttcaatctgc gccaggtcac ggctcatata
      361 cgccgtttct ttgttcgtct cgaatttacc gcgccagcga ttttccactt ggtctaagtt
      421 ggcatagatc gcatccagcg tgttgaactt ttccagcaag gcaaccgctg tcttttcgcc
      481 cacgcccttt acgccgggaa tattgtccga cgtatcgccg acgatggctt tatagtccac
      541 tacttgattc gggcggacgc ccagcttttt gatgacatcg gcatcggtga tgtacgtttg
      601 gtcatcacct gccagataca ccgccgtccg ctcattgaca agctgtagca ggtctctgtc
      661 tccggtaatg attttcacgc cgagaccttt ttccgccgcg agctttgcca cactgcccag
      721 cacatcatcg gcttcgtagc cgtccatttc gaggcgcgga atattgaacg catccaccat
      781 ttcacgaatg cgcgcgatct gcgaacgcaa atcatcgggc atcttttcgc gggttgcttt
      841 atattccggg aagatctggt cgcggaaggt tttgcctacg tcgaaagcaa cggcaaggta
      901 ttcaggtttt tcctgctcca acacgcgcac cagttcacgc gcaaagccgt aaataccagc
      961 tgtcggttca cctttcgaag tctgccaacg ttggctgcct ccgcctgctg tcagggcgaa
     1021 gtacatgcgg taggcgagag cgtgtccatc aatgagataa agggtagggg gcatgtcagt
     1081 atccagtcgt gtagtgacca attggaatag tgtctaaaag aaaaaacgca gagttaatta
     1141 tactctgcgt ttccttattt agaacttttg tttcagaaac ctacttcata ggtggcaggt
     1201 cttccaaccc ctggcgctga cgggtttggc gaatgtaatc gcgcaccagt tgtggcgggt
     1261 gtggttgcga accgcccata atgagatagc ccggcgccca gaaatcgcca gaggggtttt
     1321 cacgcttcat gcgcgggaaa tcgccaaaga ttttttctga agtctgctgg cgcataatgc
     1381 gcatcaagtg accgggcgat gtattcggct gcacgttcac aacccattgc aaatattccg
     1441 ggcgcacggc taagaactcc aagcgccagc cgaaagcgat gcaaatgtta ggcagccatt
     1501 cgccaaggcg gtctgagaga tcgccggtca ggtaatggga ggagaagcgc ggcaccaaca
     1561 aacaagcgta ggtcaggtga tacaagccag ccgagatcgg ctctgcggtc agtttgcgcg
     1621 ccatctcggt agaggggctt tcgcgagtgg cgtcattctc gccggggcgt ggacgtgcga
     1681 ccggtgtttc aggtcgcgcc ttgggctgag aaggagccgt cacttgcact tcatcgaaat
     1741 cgatttgcgt atcgtgatgg gcttcagact tttgagtctg tgccatgggc tgcggaaccg
     1801 aatctctgcg aacagcaggc gatgggtcgc gactaaagac cgaagcgttg gaaagcgcat
     1861 ccgaaacgcg ggtttgattc gggtcgaatg cttcttgcgg ctggggttgc gcaggtttct
     1921 tgttcggtaa attaaaatcg cgggtggtta atggctcagg gttcggagcc ggaatgtccg
     1981 cgagaatgtt cgagatgggg gggatgttta ggtcttcgtc tttgttgtta tcttcgctga
     2041 cagcgtaaga gaccgcaggt tgatttttag gttcggcttt ctcggtgcca aagtcgttca
     2101 gcaattggct tgcctgagag cggatggtgc taaagggagt ttcggcgtcg aaaacaagag
     2161 ccagcacggc gtctgaagtg aggcgcgtgg cgtagagcat gtgctccgct ttggtgcttt
     2221 cgagtcgaat gaaacgcaga aggtcagaac ctttttgtcc gtcccaattg cgggtgacgg
     2281 tttgcgctac ttctttggcg gcgttttgag agagaccgcc tgcatatgcc cacaagtcat
     2341 ttttcttggt gatcagcgcc gcttgcgccg aagattccaa ggtgagacgg gtaaggtgtt
     2401 gagccgcttt gttggggtca ttcagccacg gcatgaactg cgccgtgctc gacatcggca
     2461 tggctggagc cggagcgcca gactttgctg gttgagcaga ttgaggttga ctgatggcat
     2521 tcataaaatc agagattttg aacggtttgc gaaccatgat ccatggacgt aatgaatcaa
     2581 aggcaggcgg ggagcctgct tcttcgcaga ggataacaag gttgatgctg gggcgaatgg
     2641 tgcgtaaaga tgcgccaatt tctgcaaccc actcttcgcc aagtgcaagg tcgagcatcg
     2701 ccatcggcat tccgatctca tttgccttga cgatcgctgc agccttgttg ttgacggtag
     2761 tgactcggta gaacttggtg tcttccagcg aacggcgaat ggcttcaccg aggctactag
     2821 aaggggaaac aaccattaaa tcagcaggca tcggttaaag tgtatcactt ctgttatacg
     2881 catgcaagga aacctttggg tggtttctta tcattttagt aactcttctg gacaacttac
     2941 tgccccgcgt cgtagccgcc tgccaggtct ttgatgccca agagcgccat cagcacctct
     3001 ggcggaatat ctgcctcggg caaatccact tgataaagct ggtcattttc ggctcgctcg
     3061 cgcaggttct tccaaagcat gttccgctct tcgccctcta ccaccaagtc attcaaggtt
     3121 ttggcgttga gcaaatattc gccagtggaa tacaagaaga tcagccgttt ccatttatcg
     3181 gttcgaaccg gctctttcaa cttctccaaa tttcccaact gcaacttgaa atactcttct
     3241 tttgctcttg ggtgatcggc ttcatccttg agcaattcgc ctctggttgt caactcatgc
     3301 ccacgaactt gagcgatata ttcaacttgc ccgcctcttt cgccgaacga agcgggttgg
     3361 taaaacgcaa gataatccac actgacaact ttcggcgcgc tcttcaatgg gatgcgatac
     3421 catcccagca aacgcgcaat ttccatatcg cgcggtgtag gcatcaggca aacaaggatc
     3481 aagtccgtag gtttgagcat ggcttaatta taatgttcac catgcgactc tcattcctcc
     3541 ttctatcatg ttctctactc gttgcttgtg gaggaggggc tacgcctctt cccgcgcccg
     3601 aagtggattc aacccccata ccctcgtcca cgccccaacc gaccgccacc tcagcgcctg
     3661 tgccaaattt ccccgtcatc ggctatttgc cagattatcg cgagctgaac cccgcatggg
     3721 catcttcctt gaccgatatc atctacttct ctgccgagcc ccgcgccgat ggctcactgg
     3781 atacttcacg cttaacagaa gagacatggc aggcattggc atcgttaaag gatgaaaacg
     3841 ggattcgcgt acatatctcc attggcggct gggggcgaga tggtgccttt gtagaaatga
     3901 gcgccgaccc tgaaactcgt aaaaccttta ttgctaactt gttgagcttt ctacaagagc
     3961 atcagcttga cggtgccgac ttcgattggg aatttccaca aaacgacaca gagtttgaac
     4021 agtacatagc tttattaacc gaaaccaaag ctgctctttc cccgcatggc ttgttggtga
     4081 gtgttgccct cccagctgag tcgagtttcc cacttggaga gtttgcggtt gttgatcgtg
     4141 tgcatcttat gtcttatgac cgcggcgaac gacactccac ctttgagcaa tccgtggcag
     4201 atgtggagga ttttttagaa gcaggtattc cacctgagaa gttgatctta ggcatgcctt
     4261 tctatgggcg tggaatgtct tcgccatctg atacctattc ttatgaacag atacggtcta
     4321 cttatgcgcc tgcaccagac gtggatgaag tgggggatat ttatttcaac ggattgaaca
     4381 ccattcagca caaagtttgt tatggcatcg cgcaaaactt gggcggattc atggtttggg
     4441 agttagggct agatacagtg gatgagagtt cattactcca acagatatat cgcttggcac
     4501 aaggagcaga gtcatgttaa ttcatccttc cccgtatgaa cgtatcactg cattgaccga
     4561 tatcttttta gggattctgg cagcatgggg tgcggctcaa ctttttgcct tcgccgattt
     4621 ccgccatcaa atttgggcat ggaccttttg tttgctggca atcgcctctt ttctcggtgc
     4681 catcgcccac ggatttgtta tgtcaaccaa aacaaatgac cggttatgga tgccgattaa
     4741 tgtagcgcta gggttgacct taggcttgtt tgttgtgggg gcattgtttg acctaagtgg
     4801 tgaaagcgtg gctcgccaag cgttgcctgt gatgatcgcg ctaggcttcg gctttttcct
     4861 cattactatc ttgaagcccg gaacctttat gaccttcatc gcctatgaag cgcttgctat
     4921 gctctttgca ttgggcatat acggctattt attttttaca tctaatttgc tgggtgtagg
     4981 gtggatggtg cttggggtgt tggtgacgat catcgctgcc gtggtacaag caaccggaaa
     5041 tgctggcaag agtatcttct ggtatttcga taataacggg gtctttcact tgattcagat
     5101 gatcgggatg gttttgttgt attttggatt aaacacgtag gggcggggtt accccgcccc
     5161 tacttttctt ttacgataac ccaacttctt tattgaactc gacgatcaac tcatcgatct
     5221 catctgcttg cgattggacg ctggtccagt aatcgggctg ataccattgg gcttgaattt
     5281 catcgtaggt cttgccttgt tgttcaaccc aggtgtaata cttgaggtta tgcacacggc
     5341 ggcggtcggg gtagcgcagt tcgagcatat tgtctgtgga ctgtccatgt aggtagcggg
     5401 caaagtctgc ggcggcgttg gcttcggtgt attcgccgaa ctcttggtgc atttcatgca
     5461 ggcgcgagcg atacagttcc attgaatcgg tcaggacggt gatcatgatg tcatcttcgc
     5521 ccatttcgta atatttggcg gtcttaatgg cagagagtac gtttgaaatg ccagagaagc
     5581 cgagcagatc aagctggttg accacgtctt cagaaacgcc catcttcacc aaataggctt
     5641 tgccattttc ttcattgaat aagcgtgaaa tgttgaccac ggcattgtca tcaatggcgg
     5701 tgaccacgtc ggtgttcttg gtgttgtgaa cccatggcac atgcttatcg ccgatgcctt
     5761 caatgcgatg tgcaccgaat ccattttcga gcaaggtggg gcattgcaat gcttcactcg
     5821 cgacgatttt gctatcggga aagagttgct tcatgtaatc gccagaggca atggtgccag
     5881 ccgaacctgt agcagatgcc atgccacgga agcgtccatt tttgccagcc acttggcgga
     5941 agacttcttc catggcgtga ccggtgacgg tgtaatgcca aagataattg ccgaattcct
     6001 ggaattggtc gaagatcatc aagtc
//
LOCUS       DAOQJE010000089         6016 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_7464_length_6016_cov_5.651401, whole genome shotgun sequence.
ACCESSION   DAOQJE010000089 DAOQJE010000000
VERSION     DAOQJE010000089.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 6016)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6016)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6016
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7464_length_6016_cov_5.651401"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1027)
                     /locus_tag="PLT08_03585"
     CDS             complement(<1..1027)
                     /locus_tag="PLT08_03585"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0043138 - 3'-5' DNA helicase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecQ"
                     /protein_id="HNE03574.1"
                     /translation="MNTPSSILKNTFGYDTFRPLQQEVIENILARRDTLAVMPTGGGK
                     SLCYQIPSLLFDGLTVVVSPLISLMKDQVEQLRAFGVPSVFLNSSLSPHEYNENMDYV
                     KRGEVKLLYLAPETLLTPRILTLLSGLKVDLLTIDEAHCISEWGHDFRPEYRQIVEVR
                     KKFPNAVCMALTATATSRVRQDIRHTLKFATTNEFIASFNRENLFIEVVPKQDPAQQV
                     RELIDRYKDQSGIIYCFSRKQVDELAAYLTTKGYSVRPYHAGLDDAERRKNQELFIRD
                     DVQIIVATIAFGMGINKPNVRFVIHHDLPKSIEGYYQEIGRAGRDGLPAHCVLLYSYA
                     DVAKLNYF"
     gene            1230..2336
                     /locus_tag="PLT08_03590"
     CDS             1230..2336
                     /locus_tag="PLT08_03590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE03575.1"
                     /translation="MINLRKTFLRAIENEGRALGTPSIKFDNRKIISSGPDQDVPNGL
                     VYFLTDASGFYLSLPPNTVGIIVHPDGTIEAKSTGGLQEVRPGLYKILYVDMHDRSDS
                     TQPVTELSLDNESITLRVLFRYRVVDPILALGIERPVETLIEHLQADLAQYIRTNNHN
                     DLADRANPQESSKILQFFAQRHATRNRLSRAISIEGIELKEFTGDSEYVGMRRNTLTQ
                     QRQTQSEIDILDRKNEIEKLKAEQKLEIDRLTAKATAETTKLRNEILRETQKQDILLE
                     NMRTQTQRRHELLVKAVDAVGQAVEHSSYSRNPSEIKTAINNLLDAIKENSPAVEQPE
                     APKVGGSAPRTPPASNQKIEDLTNTLLDLLKPRK"
     gene            2333..3388
                     /locus_tag="PLT08_03595"
     CDS             2333..3388
                     /locus_tag="PLT08_03595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE03576.1"
                     /translation="MTKSFIETTETHEGWQSVFSFQAFHYPAEEWLKMRWGFNAGALL
                     DEAMDRNRLFLESQGINETLYFDENLPTRTLVIRGIKRPGNGLQMSVLGKVNAASQEQ
                     AKQSAEKYAREILSTFPHDFHLLPTETKLAHDKMAGNDLLVKRPSIVSIQRENTFIPP
                     MRGFHYLNGFWQTSIRSNEQIWRALSNMDQATMFNINLQPTVLLEDEKELLLEIKKKV
                     LDVEEKPAIFLPYYPWVENCIKRRLAPWKKFFLLQVHIVTDQEVDENLSRSIGSALTR
                     DTDTSPLPGFHAMHPETESEAEEWIEDLRLLSLTPPQRRMDDLVDLDEAFSVFRLPLR
                     PEAGLPGANFIEPSSIN"
     gene            3394..5565
                     /locus_tag="PLT08_03600"
     CDS             3394..5565
                     /locus_tag="PLT08_03600"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HNE03577.1"
                     /translation="MNKQTPREIVHVRVTRHMPQGLSILLDNGEQGIIRAREISWKEK
                     EDSNWKDNYPIGWDGYVVALPNKKGEVPEFSIRLLEYDPWEDFFEGLGKNRILEGHVT
                     GVYEYGAFIEVDEGITGLLHKSRVPAKIQKSIHDLFWFGDKVRVVVHDVDHERRHIEL
                     SLAPVEDITGENPAGMQNQASAASEAASHHLGQKLPKKHILVVEDETPQSDAVCGWLQ
                     EMGQQVDCVQSAEDAIEFLTRVQPDFALVDVGLPGMSGTELAHHIFENYPQIQVANAT
                     DWARASEVRAELEAVMDRGGNLFYKPLVPEDLAKFLTGDVQRVEIASQEDVVPSRSAS
                     KWDAKRDIHKLLVTFRKHLGVEQVFLFSFDPAHRKVQIAERVGDGLINKNALSQLLHS
                     PVRDVAEDGDAIFVDELTEREKKRFQYLIEFSPEAVSCIGVSVPTQSSLKYALFAMDR
                     QAKSFGEELRMYVEGMSLAVGATLDQIDLRERSALMQRSALIGNLASGMIHEINNLLA
                     PLQYESNNLRKALAKIENDPTQSKYESIRNEVANIEQDIRQIVGTVGTFGKIAKKSQV
                     EILRVDEIIKDTLILLNQISKRSRVKMDFHAPEKMVVVRNQAVVLGQIFLNVCLNAIQ
                     QISEQGADHIRAIRIDMETRQETSQSEVCRILIRDTGPGIHASLWEKIFEMGFSTRVD
                     GSGIGLFVSRNLMEEIEGKIYVLDSHILHGTVFALEFPVHV"
     gene            5574..>6016
                     /locus_tag="PLT08_03605"
     CDS             5574..>6016
                     /locus_tag="PLT08_03605"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNE03578.1"
                     /translation="MNRLLILDDNERFAEVLQRSISGFEGYESETVQYVTTSEVAIEL
                     VKEATELQSPFNVLLIDQNLEAEMDGIQVMKEMLSIHSDADTIIFTGYDTPEDGLRAY
                     EAGASRYLPKPFEPEELEFVLKELMRSRKVRLAEARQRQQFRIAT"
BASE COUNT         1770 a         1331 c         1417 g         1498 t
ORIGIN      
        1 tgaaatagtt taatttggca acatctgcat agctatatag caaaacacaa tgtgcaggca
       61 gaccatcacg cccggcacgc ccaatttctt ggtaatagcc ttcaatactt ttgggcaggt
      121 catggtggat tacaaagcgc acattaggct tattaatgcc catgccgaag gcaattgtcg
      181 ctacaatgat ttgaacatca tcacgaatga ataattcttg atttttacgc cgttctgcat
      241 cgtccagccc ggcatgatac ggacggaccg aatatccctt tgtggtaagg tatgcagcga
      301 gttcatccac ttgtttgcgc gaaaagcaat agataatgcc agattgatct ttataccggt
      361 caatgagctc gcgcacttgt tgcgcagggt cttgtttggg tacaacttca atgaaaagat
      421 tctcgcggtt aaaactggcg ataaattcgt tcgtggtggc aaacttgagc gtatgccgaa
      481 tatcttgccg cacacgagag gtagcagtag ccgtcaacgc catacaaacg gcattcggga
      541 atttcttgcg cacttctacg atctgtcggt attcggggcg aaagtcatgc ccccactctg
      601 agatgcagtg cgcttcgtcg atggtcagca agtccacttt cagtccagaa agaagcgtga
      661 gaattcttgg ggtgagaagc gtttccggcg cgagataaag caacttaact tcgccgcgct
      721 tcacataatc catattctcg ttgtattcat gcggagaaag cgagctattg agaaaaacgg
      781 acggcacgcc aaatgccctt aattgctcaa cttgatcttt catgagcgaa ataagtggag
      841 agaccacaac ggtcaagcca tcaaacaata gagatggaat ctgataacag agtgatttcc
      901 ccccaccggt aggcattaca gccaatgtgt cgcgccgcgc aaggatgttc tcgatcactt
      961 cctgctgaag cggtcgaaac gtatcgtaac caaaggtatt tttgagaata gatgaagggg
     1021 tattcacaag atccacccta aaaaggacaa gatgtttatg ggtgtgattt tatcatcttc
     1081 acaggtaaaa tttgtcttta caattaaaaa caggggtaat tgtctggagt tgtcttgcag
     1141 aaactacctt taagttgtaa actcttaata atcgtgcaaa cttcgctgct taataagcat
     1201 actatccatg ggacaaggca agagggatta tgataaattt acgcaaaaca ttcttaagag
     1261 ctattgaaaa tgaaggacgt gccctaggga caccttccat caagtttgat aatcgcaaga
     1321 ttatctcctc gggtccggac caagatgtac ccaatggatt ggtttatttt ctgactgacg
     1381 ccagcgggtt ttatctatct ctccccccaa ataccgtagg aattatcgta cacccagatg
     1441 gcaccattga agccaaaagc accggcggat tgcaagaggt gcgcccaggg ttgtataaga
     1501 ttttgtatgt agatatgcac gaccggtctg actccacgca gccagttact gaactttcat
     1561 tagacaatga atctattacc ttgcgcgttt tattcagata tcgtgttgtt gacccgatcc
     1621 tcgctcttgg cattgaacgc ccagtagaga ccctcattga acatctccaa gcggatctgg
     1681 cgcagtatat tcgcaccaat aaccataatg atcttgcaga ccgcgccaac ccgcaagagt
     1741 ccagtaagat attgcaattt tttgctcaac gtcatgccac tcgcaatcgc ctctcgcgag
     1801 caatcagcat tgaagggatc gaactcaaag aattcactgg cgatagtgaa tatgtaggca
     1861 tgcgccgtaa cacactcaca cagcaacgcc agacacagtc tgaaatagat atactggatc
     1921 gcaagaacga gattgagaag ctgaaggcag agcaaaaact agaaattgac cgcctcactg
     1981 ccaaggcgac ggctgaaact acaaaactac gcaacgaaat tctacgtgag acccaaaagc
     2041 aagatattct gctggagaat atgcgcactc aaacccagcg ccgccacgag ttattggtca
     2101 aagctgtgga tgcggttggg caagccgtgg aacattccag ttattctcgc aacccaagcg
     2161 agatcaaaac tgccatcaat aatctattag atgccattaa agagaattct ccggcggtcg
     2221 aacagccaga agcacccaaa gttggcgggt ctgctccgcg cacaccacca gctagtaatc
     2281 aaaagatcga ggatcttaca aacaccttat tagatcttct taaaccaaga aaatgaccaa
     2341 gtctttcatt gaaacaaccg aaacgcacga gggttggcaa tctgtatttt cttttcaagc
     2401 atttcattac ccagccgaag aatggctgaa aatgagatgg ggcttcaatg cgggcgcact
     2461 gcttgatgaa gccatggatc gaaaccgcct attccttgag tcacaaggta tcaatgaaac
     2521 gttgtatttt gatgaaaacc taccgacacg gacattggtc attcgcggga ttaagcgccc
     2581 tggtaatgga ctacaaatgt ctgtgcttgg aaaggtcaat gctgcaagcc aggaacaggc
     2641 aaagcaaagt gcagaaaaat atgcgcgcga aattctttct acctttccgc atgattttca
     2701 cctactgccc acagagacaa aattagctca tgacaaaatg gctggcaacg acctactagt
     2761 gaaacgtcca agcatcgtat ccattcaaag agaaaatacg ttcattccac ccatgcgcgg
     2821 ttttcactat ctaaatggct tttggcaaac aagtattcgt tccaacgaac agatctggcg
     2881 cgcgctctca aacatggacc aagcgaccat gtttaatatt aatcttcaac ccacagtact
     2941 gttggaagat gaaaaagaac tattattaga aataaaaaag aaagtgcttg atgtagaaga
     3001 aaaaccagcc atcttccttc cctattatcc atgggttgaa aactgcatca agcgtcgctt
     3061 agcaccttgg aaaaaattct ttctactaca ggttcacatt gttacagacc aagaagtaga
     3121 tgaaaatctt tcgcggtcca ttggctcggc gctcacacga gatacagaca cctcaccact
     3181 gcccggcttt cacgccatgc acccagaaac agaaagcgaa gccgaagagt ggattgaaga
     3241 ccttcgtctc ttgagcctga cgccgcctca aagaagaatg gatgacctgg ttgaccttga
     3301 tgaagccttt tcagtcttcc gcctccccct ccgacctgaa gcgggtctgc cgggtgcaaa
     3361 ctttatcgaa ccttcgagca taaactaaga attatgaata aacagacacc tagagagatc
     3421 gtgcacgtcc gcgtaacccg gcatatgcca caggggcttt ctattctgct ggataacggc
     3481 gaacagggca tcatccgcgc ccgggagatc tcttggaaag aaaaagaaga ctcgaattgg
     3541 aaagacaact atcccatcgg ttgggatggc tatgtagtcg cattgcccaa caaaaaagga
     3601 gaagttccag agttcagcat ccggttgttg gaatatgacc cttgggaaga tttttttgaa
     3661 gggcttggca agaatcgaat tcttgaaggg catgttacag gggtatatga atatggcgcc
     3721 ttcatcgaag tggatgaggg gattaccggg ctgcttcata aatcgcgcgt tcctgccaag
     3781 atacaaaagt ccattcatga ccttttctgg tttggtgata aggttcgggt tgtggttcat
     3841 gatgtagatc atgaacgcag gcacatcgag ctgagtctgg ctccggttga agatattaca
     3901 ggagagaatc cggctgggat gcagaatcag gcatcggctg cgtctgaggc ggctagtcat
     3961 catcttgggc agaaactccc caagaagcac atccttgttg ttgaggatga aacgccacag
     4021 tcagatgcag tttgtggttg gttgcaagaa atgggtcagc aggttgattg tgttcaaagt
     4081 gcggaagatg ccatcgagtt cttgacgcgc gtacagcctg actttgcgtt ggttgatgtg
     4141 gggttgcccg gaatgagtgg aacggagttg gcgcatcaca ttttcgagaa ctatccacaa
     4201 attcaagtag ccaacgcaac ggactgggcg cgtgccagtg aggttcgtgc agagcttgaa
     4261 gcggtgatgg atcgcggcgg gaatttattt tacaagccac ttgtacctga agacttggca
     4321 aaatttctaa cgggtgatgt tcaacgagtt gaaatagcaa gccaggaaga tgttgtgcct
     4381 tcacgatctg catcaaaatg ggatgcaaaa cgcgatattc ataagttgct ggtcacattt
     4441 agaaaacact taggtgttga acaggttttc cttttttcat ttgacccggc acatcgtaaa
     4501 gtccagattg cggaacgggt aggtgatggt ctcattaata agaatgccct cagccagctt
     4561 ttacatagcc ctgtacgtga tgttgccgaa gacggcgacg caatatttgt ggatgagctt
     4621 actgaaagag aaaagaagcg ttttcaatat ctgattgaat tcagccctga agctgtatca
     4681 tgcattgggg tgtctgtccc aacacagtct tctttgaaat atgctttatt cgcaatggat
     4741 aggcaagcta agtctttcgg tgaagaactg cgcatgtacg tagaaggaat gtctctggcg
     4801 gtgggcgcaa cgctggatca gatcgacttg cgtgaacgat cagcactcat gcaacgctca
     4861 gcgttaattg gcaatttggc gagcggcatg atccatgaaa tcaacaattt acttgcgccg
     4921 cttcagtatg agtctaacaa tttgcgtaaa gcacttgcaa aaatagaaaa cgacccaact
     4981 caatcgaagt atgaaagtat tcgcaacgag gtggcaaata ttgaacaaga tatccgccag
     5041 attgtaggga cggtgggaac ctttggcaaa attgcgaaaa aatcacaagt tgaaatatta
     5101 cgagtggatg agattatcaa agacacactt attttgctaa atcaaatcag caaacgctct
     5161 cgggtaaaaa tggactttca tgcgcctgaa aaaatggtgg tggtacgaaa ccaagctgta
     5221 gtattagggc aaatttttct aaatgtctgt cttaacgcaa ttcaacagat ttcagagcaa
     5281 ggtgcagacc atatccgtgc tattcggatt gatatggaaa ccagacagga aaccagccag
     5341 agcgaagtct gccgtattct aattcgggat acaggaccgg gaatccatgc atcactttgg
     5401 gagaaaatct ttgagatggg cttttccact cgtgtagatg gcagtggaat tgggttattc
     5461 gtatcacgaa acttaatgga agaaattgaa ggaaaaatct acgtattgga tagtcatatc
     5521 ctacatggca cggtttttgc ccttgaattc cctgtccatg tatgaggctt actatgaatc
     5581 gtttacttat tcttgatgac aatgaacgct ttgcagaggt tttacagcgc agtatcagcg
     5641 ggtttgaggg ctacgagtct gagactgttc aatatgtcac tacgtcagaa gtggcaattg
     5701 agcttgtaaa agaagcaacc gaacttcaaa gcccttttaa tgtccttctg attgaccaaa
     5761 acctggaagc agaaatggat ggcattcagg ttatgaaaga aatgttatcc atccattcag
     5821 atgcagacac aattattttc acagggtatg acacaccaga agacggctta cgagcctatg
     5881 aagcaggagc ctcacggtat cttccgaagc catttgaacc agaagagctg gaattcgtcc
     5941 tcaaagaact aatgcgctca agaaaagtgc gtctggcaga ggcacgtcaa cggcagcaat
     6001 ttagaatcgc tacaaa
//
LOCUS       DAOQJE010000090         6014 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_7466_length_6014_cov_7.019466, whole genome shotgun sequence.
ACCESSION   DAOQJE010000090 DAOQJE010000000
VERSION     DAOQJE010000090.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 6014)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6014)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6014
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7466_length_6014_cov_7.019466"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..486
                     /locus_tag="PLT08_03610"
     CDS             <1..486
                     /locus_tag="PLT08_03610"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015679.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_function: GO:0071972 - peptidoglycan L,D-transpeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HNE03579.1"
                     /translation="YIPSYNMRLIPNEELTQISPEVPPEEKLLHVDIATQMVTAFEGE
                     KVVLSVRCASGQHGTDTPKGEWSTYHKGPSIHMTNQGDAVANIYNLPGVPWCSFFTGS
                     GHAFHGTYWHNDYGRLRSNGCVNLPSSVAKWVYRWTNPVVPYNVDYVNLPGQGTKVII
                     E"
     gene            547..1122
                     /locus_tag="PLT08_03615"
     CDS             547..1122
                     /locus_tag="PLT08_03615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE03580.1"
                     /translation="MNQIFQGNQYLLKRQVFALTGKFRVFDTAGRLLLFSEQKMFKLR
                     EDIRVYADEAKTQEVLAIKARQIVDFSAAYDVIDSATGQKVGAMRRKGLKSILRDEWE
                     VLDVNDNVRGVLVEDSMGLALLRRFLTNLVPQNYDMLFGETRVGDFKQNFNPFTYELN
                     IDFSMDTAGQVDRRLGIAAGILLAAVEGRQQ"
     gene            1192..1617
                     /locus_tag="PLT08_03620"
     CDS             1192..1617
                     /locus_tag="PLT08_03620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE03581.1"
                     /translation="MPQPLFAGLIVDEDGNPVQAASIGSEPAYVIDDAGFKRHIPAEQ
                     VDRAVLNQMADMMKGSEDVLSEQTAKMLGQEDPFSKAMIANQLKNIDKQFDALMKTGF
                     PEELRAYLGMMGFKIVINYHGEVVRVEQPGTSGGDEGDE"
     gene            complement(1652..2386)
                     /locus_tag="PLT08_03625"
     CDS             complement(1652..2386)
                     /locus_tag="PLT08_03625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNE03582.1"
                     /translation="MTERDMNKAALRGEPSYVWRDGQKRRLNMIAKFAAERMQGKVLE
                     NGCGVGMYVEKMTELGATVTGLEYDFERACDAHVNSPRIVNAAGEFLPLPSSTFDLIL
                     SHEVIEHVQDDRSAIAEMVRVMKVGGRAVIFCPNRGYPVETHGIFWNGKYYFGNKLFV
                     NYLPRVWRDKLAPHVRVYSRRDMQQLFEGLPVKFIERTIIFGAYDNIIARLGVFGKFI
                     RAVLQFMEATPLKILGLSHLWVVEKI"
     gene            2580..>4191
                     /gene="nusA"
                     /locus_tag="PLT08_03630"
     CDS             2580..>4191
                     /gene="nusA"
                     /locus_tag="PLT08_03630"
                     /inference="COORDINATES: protein motif:HMM:TIGR01953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusA"
                     /protein_id="HNE03583.1"
                     /translation="MAKNDFALAFNEVLEEKQLAKDVVITAIESAMVSAYRRAVGAST
                     AQYVESKLDPETGIVTVYAEKEVVEDSIVDDRTEVLLEVARKVNPECQPGDMVVVEST
                     PADFGRVAAQTARQVIQQRIREAERSNQMEYYQRQEGEIVSGLVQAINAQGITIGLDM
                     KAEGVMPNNQRIPGEKLKLHDRVRAVVMEVKDGTRGPQIILSRAHRNFLRRLMENEVP
                     EIYHGIVEIRAIAREPGARAKVAVSATQPGIDPVGACVGQKGVRIQAIVKELHDEKID
                     IILWDADPVAYISKAISPAKVTGVYLTEEERTATVVVGEDQLSLAIGRDGQNARLAAK
                     LTSWRIDIKSLSEAAGDSLAKLQGDAELQNMLKAAVEVMPQVEEILAKKAENRLITPE
                     EFSTLEQFVDRVERRMILAKEEASREEDERIAAARAEIPAAAFNMPLDQATNLKEHVF
                     NILTEAGYETVGQLIFDMKTDQNKVLGLAGIGGKAITNIEETLAALTFPEPVAEPKVE
                     ETPAVVAEPVVTETAPVVETPVVEKPAEK"
     assembly_gap    4192..4291
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <4292..4609
                     /locus_tag="PLT08_03635"
     CDS             <4292..4609
                     /locus_tag="PLT08_03635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE03584.1"
                     /translation="TETAPVVETPVVEKPAEKKKEPKRVIEEEEEDGEVSKDGVNLDE
                     LFQMKPEMFQAGEGADEESDDKKKGKKVKKKGVALEFDENLGEVVSRKKHKRSDGGDD
                     EAW"
     gene            4654..4953
                     /locus_tag="PLT08_03640"
     CDS             4654..4953
                     /locus_tag="PLT08_03640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxR family protein"
                     /protein_id="HNE03585.1"
                     /translation="MVKKPVQRVKHIPQRTCVGCREILSKRQLLRIVRTAEGVRVDPT
                     SKLAGRGAYLHDKRSCWARGLKGGLAHALKTEISAEDRARLEEFMNTLPEAETDG"
     gene            5038..>6014
                     /gene="infB"
                     /locus_tag="PLT08_03645"
     CDS             5038..>6014
                     /gene="infB"
                     /locus_tag="PLT08_03645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HNE03586.1"
                     /translation="MSENGNKIELPASIVVRDLAQKIEKSPIDLIKKLMTNGVMATIN
                     QTVDYDTAAIVVAEYGFEAVPEVVEEESKEEAGEVPMWRQMIAGEDKAQLKQRPPVVT
                     ILGHVDHGKTSLLDAIRSTDVAAGEAGGITQHIGAYQVEMKGRLITFLDTPGHAAFTQ
                     MRARGAQGADIVILVVAADDGVMPQTKEAIAHAKAARVPIVVALNKVDKPNANPDRVK
                     QQLAELELVPDDWGGNTMVVPVSAKQKKGIDDLLEGVLLVADSNEIKANPSGKVIGTV
                     IEAELDKSKGVLATLLVQNGTLESGDVVVAGTAHGKLRAITDYKGKPVK"
BASE COUNT         1582 a         1457 c         1583 g         1292 t
ORIGIN      
        1 tacattcctt cttataatat gcgcctaatt cccaatgaag aattaacgca aatttctccc
       61 gaagttccgc ccgaagaaaa attactgcat gttgatattg ccacgcaaat ggtgaccgcc
      121 ttcgagggcg agaaggtcgt tctctccgta cgctgcgcaa gtggtcagca tggaaccgat
      181 acgcccaaag gagaatggag cacctatcat aaaggtccat ccattcatat gacaaaccaa
      241 ggcgatgctg ttgccaacat ctacaaccta cccggggtgc cgtggtgctc cttcttcacc
      301 ggctctgggc atgccttcca tggaacctat tggcacaatg attatggtcg cctccgcagt
      361 aatggatgtg tcaatttgcc ctccagtgtc gcaaaatggg tctaccgttg gacgaaccca
      421 gttgtgcctt acaacgtaga ttatgtgaac ctgcccggtc agggcacgaa agtaatcatt
      481 gaataggttg gcacgaacgt aaaagacgtt cgataaattt tttagaatca aaaaaaggac
      541 agagagatga atcaaatttt tcaaggcaac caatatttac tcaaacgcca ggtcttcgca
      601 ctcactggta aattccgcgt gttcgacacc gccggcagat tgctgctctt cagcgagcag
      661 aagatgttca agttacgcga agatattcgc gtatatgctg atgaagccaa gacacaagaa
      721 gttttggcga tcaaagctcg ccagatcgta gatttctcgg ctgcatatga tgtgattgac
      781 agtgccacag gtcagaaagt gggagccatg cgccgcaaag gtcttaagtc gattctgcgc
      841 gatgaatggg aagtgctgga tgtcaacgac aatgtgcgcg gtgttttggt cgaagatagc
      901 atgggtcttg ctttactgcg ccgcttcttg accaacctcg tgccgcagaa ctacgacatg
      961 ctcttcggcg aaactcgcgt aggcgatttc aaacagaatt tcaatccctt cacatatgaa
     1021 ctgaacatcg acttcagcat ggatacggcg ggtcaggttg accgccgtct tggcattgcc
     1081 gctggcattt tgcttgctgc ggttgaaggt cgccaacaat aatttcttga tatctttcct
     1141 ctttcttctt tcttgcaaaa caaaagagga aagaagaaag aggagttctt aatgccccaa
     1201 cctctcttcg ccggtctcat cgtagatgaa gacggcaacc ccgtacaagc cgcatccatt
     1261 ggcagtgaac ctgcttacgt aatagatgat gccggattca aacgtcacat ccctgccgag
     1321 caagtggatc gcgctgtgct caaccaaatg gcagacatga tgaaaggcag tgaagatgtc
     1381 ctttcggagc agactgccaa aatgcttggg caggaagatc ccttctcaaa ggcgatgatc
     1441 gcgaatcagc taaagaatat agataagcaa ttcgatgcct tgatgaaaac aggctttcct
     1501 gaagaactgc gtgcctattt agggatgatg ggtttcaaga tagttatcaa ttaccacggt
     1561 gaagtggtac gagtggaaca gccggggact tccggtggcg atgagggtga tgaataaaga
     1621 aaaactccga ggtgttgaac ctcggagttt tttatatctt ctctaccacc caaagatggg
     1681 ataaacccaa tatctttagc ggtgtcgctt ccataaattg caacacagca cggataaatt
     1741 ttccgaagac acccaagcgg gcgatgatgt tatcgtaggc accaaagata atggtgcgtt
     1801 caatgaattt gacaggtaat ccctcaaata gttgctgcat atcacggcgg gaatagacgc
     1861 gcacgtgcgg ggcaagcttg tcgcgccata ctctgggtag gtaatttaca aacagcttat
     1921 taccaaaata atatttgcca ttccagaaaa taccatgggt ttccacgggg tatccgcggt
     1981 tggggcagaa gatgacagcc cgcccaccca ctttcatcac gcgcaccatt tcggcaatcg
     2041 cggagcggtc atcctgtacg tgttcgatca cttcgtggct gagaatcagg tcaaacgtcg
     2101 aagaaggaag cgggaggaat tccccggcgg cgttgacgat acgcggcgaa ttcacatggg
     2161 catcacaggc gcgttcaaag tcatattcca acccggtgac tgttgcccca agttcggtca
     2221 tcttttctac gtacatgcct acgccgcagc cattttccag cactttgcct tgcatgcgtt
     2281 ccgcggcaaa ctttgcaatc atgttcaaac ggcgcttttg tccatcgcgc catacatacg
     2341 aaggttctcc gcgcagggcg gctttgttca tatctcgttc ggtcatggcg gcgattatac
     2401 actgccaagt tcaaatgctc ctctaacaga ctcagccttg acgctgaaac ctttctgcta
     2461 tagaatctcg ctcgtaaggg acaacccgaa tagtgaacgt caccgagaag tgggcagccc
     2521 ccacttcttc gctttatacc ctatgggtat gatatctaaa ggagttttgg agcagtacga
     2581 tggcaaagaa tgattttgcg ctagcattta acgaagtgct ggaagaaaag caactcgcca
     2641 aggacgtggt catcaccgcg atcgagtcgg cgatggtttc cgcataccgg cgggctgtgg
     2701 gggcatccac cgctcaatat gtggagtcca agctggaccc cgaaacgggg attgtaaccg
     2761 tctatgctga gaaggaagtt gtagaagata gcatcgtcga tgatcgcact gaggttcttc
     2821 tcgaggtagc ccgcaaggtg aaccctgagt gccagccggg cgatatggtc gtggtggaaa
     2881 gcaccccggc tgatttcggt cgtgtggcgg cacagacggc tcgtcaggtc atccagcagc
     2941 gtattcgtga agcagaacgc tccaatcaga tggagtatta ccagcgccag gaaggcgaaa
     3001 tcgtctctgg tttggtacag gcgatcaatg cacagggcat aactatcggc ttggatatga
     3061 aagccgaagg cgtgatgccg aacaatcagc gcattcctgg tgaaaaattg aagctgcatg
     3121 atcgcgtccg cgcggtggtg atggaagtca aagacggaac ccgtggtccg cagattatcc
     3181 tttcacgcgc gcatcgtaac ttcttgcgcc gcttgatgga aaacgaagtg cctgagatct
     3241 atcatggcat tgttgagatc cgcgcgattg cccgcgaacc aggtgcccgc gccaaagtgg
     3301 cggtttctgc cacgcaaccc ggtatcgacc ccgtcggcgc atgtgtgggt caaaagggtg
     3361 ttcgtattca agccatcgtc aaagaattgc acgatgagaa gatcgacatc attttgtggg
     3421 acgccgaccc ggttgcctac atctccaaag ccattagccc cgccaaagtg actggcgtat
     3481 atctcaccga agaagaacgc acagctaccg ttgtagttgg cgaagaccag ttgagtcttg
     3541 ccatcggtcg tgatggacaa aatgcccgcc tcgctgccaa actgaccagc tggcgcatcg
     3601 acatcaagtc tttgtctgaa gctgcaggcg actcgcttgc caaacttcaa ggcgatgccg
     3661 aattgcagaa tatgctcaag gctgcggtgg aagtgatgcc gcaggtggaa gaaattctcg
     3721 ctaagaaggc tgaaaatcgc ctcattacgc cggaagaatt cagcacactc gaacaattcg
     3781 tggaccgtgt ggaacgccgc atgatccttg ccaaggaaga ggcttctcgc gaagaagacg
     3841 aacgcattgc cgccgctcgc gccgaaatcc ctgccgctgc gttcaacatg ccgctcgatc
     3901 aggcaacaaa cctcaaggaa cacgtcttca acattcttac tgaagctggc tatgaaaccg
     3961 ttggtcagtt gatcttcgat atgaagaccg accagaacaa agtgttgggg ctggctggta
     4021 ttggtggtaa agccattacg aacatcgaag aaacgctcgc ggctctcacc ttccccgaac
     4081 cggttgctga acccaaggtg gaggaaaccc cggctgttgt tgcagaaccc gttgtgacag
     4141 aaacggctcc agtggtggaa acgcctgttg tggagaaacc tgccgaaaag annnnnnnnn
     4201 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4261 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nacagaaacg gctccagtgg tggaaacgcc
     4321 tgttgtggag aaacctgccg aaaagaagaa agaaccgaaa cgcgtcatcg aagaagagga
     4381 agaagatggc gaagtctcga aggacggcgt caacctcgac gagctgttcc aaatgaagcc
     4441 agaaatgttc caagctggcg aaggtgcgga tgaagaatcc gacgataaga agaagggcaa
     4501 gaaagttaag aagaagggtg ttgccctcga gttcgacgaa aaccttggcg aagttgtctc
     4561 acgcaagaag cacaaacgca gtgatggcgg cgacgacgag gcatggtagt taatgattcc
     4621 cgcctccata atggaggcgg ggagtaaacg gacgtggtca aaaaacctgt acaacgcgta
     4681 aaacatattc cgcagcgcac ctgtgtcggg tgccgggaaa tcctgtcgaa acgacaatta
     4741 ctgagaattg tccgcacagc agaaggcgtg cgtgtggacc ccaccagtaa gttggcagga
     4801 aggggcgctt acttgcacga caagcgctct tgttgggcgc gcggattaaa aggcggttta
     4861 gcccacgctt tgaaaacgga aattagcgca gaagaccgcg cccgtttgga agaatttatg
     4921 aacactctgc cagaagcaga gactgacggt taagccatgt gaacgaataa gccactcgct
     4981 cacaggctgc ctgatacatt gaacaaagat atgttttgag attcaaggag gtggcttatg
     5041 agtgaaaacg gaaataagat cgaattacct gccagcattg tagtacgaga cctggctcaa
     5101 aagatcgaaa aaagcccgat tgacctgatc aaaaaattga tgaccaatgg tgtcatggca
     5161 accattaatc agacggtgga ttacgatacc gccgcgattg tggttgctga gtatggcttt
     5221 gaagccgtac ccgaagtcgt tgaagaagaa tcaaaagaag aagcgggcga agtgcccatg
     5281 tggcgtcaga tgattgccgg ggaagataag gctcaactga aacagcgccc cccggttgtg
     5341 accattctgg gtcacgtcga tcacggcaag accagcttgt tggatgccat tcgttccacg
     5401 gatgtggctg ctggtgaagc tggcggtatt actcaacaca tcggcgcgta tcaagtggaa
     5461 atgaagggac gtctcatcac cttcctcgat acgcccggtc acgccgcgtt cacgcaaatg
     5521 cgcgctcgtg gtgctcaagg tgctgatatc gtcatcttgg tagtggctgc cgacgatggc
     5581 gtgatgcccc aaaccaaaga agccattgcg cacgccaaag cggcgcgtgt gccaatcgtg
     5641 gtggcattga acaaggtcga taaacccaac gccaacccag accgtgtaaa acaacaactt
     5701 gccgaactcg aactcgtccc tgatgattgg ggtggcaata ccatggtcgt gcccgtctct
     5761 gccaagcaga aaaaaggcat cgacgatttg ctcgaaggcg ttttgctcgt ggcagatagc
     5821 aacgagatca aagccaaccc cagcggcaaa gtaatcggta ccgtcatcga agcggaattg
     5881 gataaatcca agggtgtgct ggcgaccttg ctcgttcaga acggtacgct cgaatcaggc
     5941 gacgtggtgg tagctggaac cgcgcatggc aaattgcgcg ccatcaccga ttacaagggc
     6001 aagccggtca agaa
//
LOCUS       DAOQJE010000091         6005 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_7482_length_6005_cov_6.098151, whole genome shotgun sequence.
ACCESSION   DAOQJE010000091 DAOQJE010000000
VERSION     DAOQJE010000091.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 6005)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6005)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6005
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7482_length_6005_cov_6.098151"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            379..978
                     /locus_tag="PLT08_03650"
     CDS             379..978
                     /locus_tag="PLT08_03650"
                     /inference="COORDINATES: protein motif:HMM:NF012451.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaJ domain-containing protein"
                     /protein_id="HNE03587.1"
                     /translation="MYNENSTRSYPLYWPEGWPRTERHKIKRAQFKDRSVFTARKQLE
                     LEVRRFGGRELIISSNLELKLDGMPRSGQKQPADKGVAIFFTRNKVEMALACDVYSTV
                     EDNLWALVRTLDALRQIERDGSPALINKAFKGFAALPDPNERNWWEVLGVAANADDDQ
                     IKTAYRDLARRYHPDNAESGNIDTFHQIQKAYEQAVGRK"
     gene            complement(1634..1882)
                     /locus_tag="PLT08_03655"
     CDS             complement(1634..1882)
                     /locus_tag="PLT08_03655"
                     /inference="COORDINATES: protein motif:HMM:NF024252.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HNE03588.1"
                     /translation="MSVTVNNRFAILLAEKRIKERRNISLAEVADEIGISRKTLYAWE
                     NNTVTRFDVPVIDALCQYFSVKPGDLFEYIEEEQPKKK"
     gene            2021..2380
                     /locus_tag="PLT08_03660"
     CDS             2021..2380
                     /locus_tag="PLT08_03660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE03589.1"
                     /translation="MNSQNRLTKSEIFTVYNQMRAAARREGNLKLIERLNKAVGILLS
                     KEYYQGERLAYIPTAHSCGCHDWEFRNTAKRGYTGGCKHMLAEAMMQSALALRAAHEV
                     TVWLTPRESFPAEVAVR"
     gene            2710..3054
                     /locus_tag="PLT08_03665"
     CDS             2710..3054
                     /locus_tag="PLT08_03665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0070064 - proline-rich region binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HNE03590.1"
                     /translation="MAKKIFPLLVLLSAIAYGLVWLMPDVEPENASPIVIPSSQTLQS
                     PMPATPGDFCKVVTGLETGRVNLRECAGTTCPVLRVLEDGQMLTVVKAGAWNEVTTVD
                     GVRGWINSTFCK"
     gene            3064..4092
                     /locus_tag="PLT08_03670"
     CDS             3064..4092
                     /locus_tag="PLT08_03670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE03591.1"
                     /translation="MVRKIGTIVVFILAAVLLVYSATRSLDFISLTLPEDRKVLAYFG
                     LAALDGGLVAWILSYLYGSKGWQRAVSFLMVLVDLVGAIAMFTLDTLYNTGKAGMTEA
                     LTPSEMQTAVLGLSGVIALNIAAAVAHHLTDPEKLREQAEEDAFSKVEDATLKQISKN
                     ADSLAAQLAPTLAQDWMNQTRSRYMSYVGTGQIPTLIDSTAQNVPASSSAPVPFAMLQ
                     DNPKQTVSFMQFLPAWLTTKFGGGRKFEAAVPAPSVELKSHSEMFLYKCPSCGWEIKT
                     PEKFTNTKPCMNCDGFIELQTLKPQNNSLYEAFKQGIAEAQGVESADLHSPLGGSDAV
                     PTEGPEPK"
     gene            4374..4568
                     /locus_tag="PLT08_03675"
     CDS             4374..4568
                     /locus_tag="PLT08_03675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE03592.1"
                     /translation="MKVEIYSIDTNETISENTHQSVADAEKFIALIKEYGFEDTNGNR
                     HKFSDAKVTPQGAVVYVEKE"
     gene            4889..5845
                     /locus_tag="PLT08_03680"
     CDS             4889..5845
                     /locus_tag="PLT08_03680"
                     /inference="COORDINATES: protein motif:HMM:NF012798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HNE03593.1"
                     /translation="MTKSNAIIAQASSEIQIDQNAAAIYIASLGSESGRRTMLQVLNL
                     IAGSISRNENAMTMDWSSLRFQHVAAVRSKMQEHYKPATVNKALAALKGTLRASWQSG
                     QMSAEDYQKAVSVKGVRGETIPTGRELSSGELAALLSSCEEDPSPAGVRDAAVIAVMY
                     SCGLRRAEVVTLDYADYDDESGRLAIRGKGKKERTAYITGGAALALNDWLSLRGSNPG
                     ALFVAVNKAGVLGDARITSQAIYNMLQKRASEAGVKPFSPHDMRRTFVSDLLDSGADI
                     ATVSKMAGHANVTTTARYDRRPEDAKRKAASLLHVPYHGRGA"
BASE COUNT         1588 a         1357 c         1615 g         1445 t
ORIGIN      
        1 gcgcagaggg tatcaggcgt gtgaaagagt ccccgatcga aattgtagag agtcttcttc
       61 tgtgacctgt gaggaccgtt gctgtagaaa cagtgagcac cgacacgggt cggcgagagc
      121 tggtaactgt gtcggcagtg tatcggtgag aacgactctt gaaagctttt gatgtggttt
      181 ttcaaaggca gaagggaggg gtacaaaaaa gttttggctc ggggaatgta gaccggaggg
      241 gttcctcggc gtgaaaaaaa acccgaatgt gagaaatttc cggcggcttc tgttgctgtg
      301 gaaacgatga cctggtcagc gtctatggcg tcggattgat catcaaaaaa attctgaaca
      361 aaaaaggagt aagaaaaaat gtataacgaa aattcaacac ggtcctatcc cttatattgg
      421 cccgaaggat ggccacgaac cgagaggcat aaaatcaagc gagcacaatt caaagaccga
      481 tcggtgttta ctgcgcggaa gcaattggag cttgaggtcc gccggtttgg tggtcgagag
      541 ttgattatct catccaacct ggaattaaaa ctggatggga tgccgcggtc ggggcagaaa
      601 caaccagcgg ataaaggcgt cgcaatattt ttcactcgaa acaaagtcga gatggcattg
      661 gcttgtgatg tgtattcaac tgttgaagat aatctctggg cattggttag gacattagat
      721 gctctacgcc aaatagaacg cgacggatca ccagccctca tcaacaaagc attcaaggga
      781 tttgcagcat tgccggaccc gaatgagcgc aattggtggg aggtgctagg ggtggcagct
      841 aatgcagatg atgaccaaat caagaccgca tatcgtgacc tggctcgacg atatcacccc
      901 gacaatgcgg agtctggcaa tattgatacc tttcaccaaa tccaaaaggc ttatgaacag
      961 gctgttggta ggaagtagga atggtgacca ggtcattagt tacgtcgccg gtttgatcat
     1021 cacctcaagc catcacatca tggagctgtc ggcatttgaa tttctgagcg tgtgagagtc
     1081 tgtcggctag ctgtgtatat tatccacacc ggtgagggat ggactctcgc ggcttatgca
     1141 atttgaattg ccatgctcga ggatccttga ttatttgttt tggtaggtgg gtgagagtcc
     1201 ttgctttgat gtcggcatga tatccgggca cgactctcgc cgacttttcg ttcctgggcg
     1261 gtggcgaggt tttggaagcc agaaaaaaaa tctcagtttg aaaatagaac aaatttgcta
     1321 ataatctttt gaagtgtttc cacggaaaca ggaccgatcg gtggcgagag ccgtcttcat
     1381 aaaagcagac aagtactttt gcgacaataa ataatgtacc cccgtcacgc cactttcatt
     1441 agttcatcaa agcgctcatt gccggagtcg tatggttgac ctggtcgcgc tggtggatct
     1501 tcatcacgtg gtgtttctac agaaacggtg agaccggtcc ggatatcggc gccggcatcg
     1561 atgaccagac ataggcaaag taaaagccgc ctcgtgagca cgaggcggct tgttgttggt
     1621 ggggcggtgg agtctatttc ttttttggct gctcttcttc gatgtattca aacagatcac
     1681 caggcttgac actgaagtac tgacagagag catcaatcac gggcacatca aagcgagtaa
     1741 cagtattgtt ctcccaggcg tagagggtct ttcgagaaat gcctatttcg tctgcgactt
     1801 cagcaaggga gatgttgcgg cgctctttta tgcgcttttc ggcgagaagg atggcgaagc
     1861 gattattgac ggtcacactc atattttact ccatttgtac actagtgtta cagatttttg
     1921 acccttgaca tgctgttacc ctagggttac aatagcgaaa gtttagaaac agtaactatt
     1981 gtaacacaaa agtaagaacg gaaaacgaag gagataagcg atgaatagcc agaaccgcct
     2041 caccaaatcc gagatattca cagtctacaa ccagatgcgc gctgcggctc gccgcgaggg
     2101 aaatctcaag ttgattgaac gcttgaacaa ggctgtcggc attttgctga gcaaggaata
     2161 ctaccaaggt gaacgcctcg cttatatccc gaccgctcat tcctgtgggt gccacgattg
     2221 ggaattccgc aacactgcca aacgtggtta caccggtgga tgcaaacaca tgcttgctga
     2281 agccatgatg cagtcggctc ttgcgcttcg cgctgctcat gaagtcacag tctggcttac
     2341 tccgcgagag tcgttccctg cggaggtcgc agttcgataa cctagtggat accagcgcta
     2401 ctggcagcgc tgttccaagc ccgcctgtgt ccttcgcatg ggcgggcaac cttatctcag
     2461 aagaggagca tccaatgaga ccaaagccaa gaccaagaaa aagaaagccc aggagagtcc
     2521 tgggagattg gaggaagtta tttgaccaaa tgacaaaagg ggagaggctt gaaatggcat
     2581 gtctgatgtt gaagacaatc gagagtcgtc agcatcgcgg aataaaaaag cccgctcagg
     2641 aaatcggtac ataacctaag cgggcaccgt gggaagacgg tacgtttatt ttaccctgag
     2701 gaggtaacct tggctaagaa aatttttcct ctgcttgtgt tgttgtcggc gattgcttac
     2761 ggtctcgtct ggcttatgcc agacgttgaa ccggaaaacg catcaccgat cgtaattcca
     2821 tcatcacaga ctctacagtc accaatgccg gcaacgccag gcgatttctg caaggtggtg
     2881 actggtctcg aaacaggacg tgtcaacctg cgcgaatgtg cagggacgac gtgtcctgtt
     2941 ttgcgtgttc tggaagatgg tcagatgttg accgtagtca aagccggtgc gtggaacgaa
     3001 gtcacaacag tggacggcgt tcgcggctgg ataaattcaa ccttttgtaa ataggagaag
     3061 tatatggttc gcaaaatcgg tacgatagtt gttttcattt tggcagcggt gctgttggtg
     3121 tattctgcga ctcgcagtct cgacttcatc agtctcacgc ttcctgagga ccgcaaggtc
     3181 cttgcttatt ttggtctcgc tgcgttggat ggtggtctcg tcgcgtggat cttgtcctac
     3241 ctgtatggct ccaagggatg gcagcgtgct gtttcgtttt tgatggtgtt ggtcgacctg
     3301 gtcggcgcta ttgccatgtt cactttggat actctctaca acactggtaa ggcgggcatg
     3361 actgaagctc tcacaccttc tgaaatgcag accgcagtcc tgggattgtc cggagtcatt
     3421 gctttgaaca tcgccgcggc tgttgctcat cacctgacgg atccggaaaa gctgcgcgag
     3481 caagctgaag aagacgcatt cagcaaagtt gaagatgcga ctctcaagca gatctcaaag
     3541 aatgcggact ctctcgcggc tcagcttgct ccgactcttg cccaggactg gatgaaccag
     3601 acacgctctc gatatatgtc ctatgtcggc actggtcaaa ttccgacctt gattgatagc
     3661 accgcgcaaa atgtgccggc atcgagcagc gctcctgtgc ccttcgccat gttgcaggat
     3721 aacccgaagc aaacagtcag cttcatgcag tttcttccgg cgtggctcac tacgaagttt
     3781 ggaggtggtc gaaagtttga agccgcagtg cctgcgccat ccgttgaatt gaagtcgcac
     3841 agtgaaatgt ttttatataa atgcccttca tgtggatggg aaataaaaac ccctgaaaaa
     3901 tttaccaata ctaagccttg catgaattgt gacggtttta ttgaattaca aactttgaag
     3961 ccacaaaaca attctttata cgaagccttc aagcaaggca ttgctgaagc acaaggcgta
     4021 gagtctgctg atcttcattc tccgcttggt ggaagtgacg cggtccctac cgaaggaccc
     4081 gaaccgaagt aggaataaga gtctgtgctc gctgcggtct cgactatgtc ggcgctttac
     4141 atggacgtta ctgttcgcag tcctgcaagc aaaaagcata tcgagaccgc aagcggcacg
     4201 gcggaacggg tcccaaaaaa tcagatctcc ggacttgtgc tcgatgcgga atacaattcg
     4261 aaggtgctct tcatggtcga taccatagcc aggcatgcaa acaggcagcc tacagagagc
     4321 gtaagcagcg ccagaaccaa atggcgactt tgaattgaac aaaggagata accatgaaag
     4381 tagaaatcta tagcatcgat acgaacgaaa caatttcaga gaacactcat cagtccgttg
     4441 cagatgcaga gaaatttatt gctctcatca aagaatatgg ctttgaagat accaatggca
     4501 atcgccacaa atttagtgat gccaaggtga ccccgcaagg tgcagttgtg tacgttgaga
     4561 aagaataatt atttggcaag ttgaaagcct gaaccttaac aacaaaatat cgtcatctcc
     4621 cagccgtggg aagcggacag actctcgaag tgatttcgat ggtgagtact caaaatgcgg
     4681 gaaggtgacg aaacgacaat ataataaaaa agccttgtag aatgcgtgag agtcgtctgg
     4741 gtggagaata tcccacagga cagacggagc gagtcgctac gggcttcctg acacgaaaga
     4801 gggcatgtcc agcaatggac atgccctaaa aatgctgttt ttcagttatg agaatggcac
     4861 ttctgaaaat aaacgaaagg agaacggaat gacgaagagt aatgcaatca ttgcccaggc
     4921 aagcagtgaa attcagatcg accaaaacgc tgcagcaatc tacattgcaa gtcttggaag
     4981 tgaaagcgga cggcggacca tgctccaagt gttgaacctc attgccggta gtatctctcg
     5041 caatgagaac gctatgacta tggactggtc atcccttcgc tttcagcatg tcgcggctgt
     5101 tcgttcgaag atgcaggaac attacaagcc tgcgacggtc aacaaagctt tggcagctct
     5161 gaaagggact ctgcgggctt catggcaatc agggcagatg agcgctgagg attatcaaaa
     5221 ggctgtgagt gtgaaaggcg tccgtggcga gaccattcca acagggcgtg aattgtcgtc
     5281 cggagagttg gcagcgttgc tttcttcctg cgaagaggat ccttcacctg caggtgtgag
     5341 agatgcggcg gttattgcag tcatgtattc ttgtggattg cgtcgggctg aggtagtcac
     5401 acttgactat gccgactacg acgatgaaag cggacgcctg gcgatacgtg gtaagggcaa
     5461 gaaagaacga acagcataca tcaccggtgg tgcagctctc gcgttgaatg actggctgag
     5521 tcttcgagga agtaacccgg gagcgttgtt tgtcgcagta aataaagcgg gagtcctggg
     5581 agatgcacgg atcacttctc aagcaatata caacatgctt cagaaacgag cgagtgaggc
     5641 aggcgtaaag cctttctctc cgcatgatat gagacgaacc tttgtcagtg acttgctcga
     5701 ctctggtgcc gacatcgcta cagtctcgaa gatggctggt catgccaacg taacaacaac
     5761 ggcacgctat gaccgccgac cagaggacgc gaagagaaaa gctgcgtccc tcttgcacgt
     5821 gccctatcat gggcgtggtg cataaaaata gagcgactga ctctttcaag tcagccgatt
     5881 gtttaccgtt ctgtatccta gatatgggtg tttgttagag gcattgcccg tgtatttgct
     5941 acttcgataa ttttccctct tagattatcc gaagtcaggc gctctatcca ttgagctact
     6001 ggggc
//
LOCUS       DAOQJE010000092         5938 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_7611_length_5938_cov_4.293388, whole genome shotgun sequence.
ACCESSION   DAOQJE010000092 DAOQJE010000000
VERSION     DAOQJE010000092.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 5938)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5938)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5938
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7611_length_5938_cov_4.293388"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..223)
                     /locus_tag="PLT08_03685"
     CDS             complement(<1..223)
                     /locus_tag="PLT08_03685"
                     /inference="COORDINATES: protein motif:HMM:NF012787.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin domain-containing protein"
                     /protein_id="HNE03594.1"
                     /translation="MKNSRFITWMTWALILGMFWTVFSRVSPDQPKAQQDAVAKEGFT
                     APNFTLDLLDGSTVALSDLRGKVVLVNFWT"
     gene            complement(223..537)
                     /locus_tag="PLT08_03690"
     CDS             complement(223..537)
                     /locus_tag="PLT08_03690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE03595.1"
                     /translation="MKIELLYFDGCPSWEAGLESLRTALQEEGISASVEMVKVETDED
                     AERLKFLGSPHFRVDGMDLWHEERETYSMSCRVYSTPEGVKGFPTVHMLREKLQSLSG
                     TP"
     gene            complement(544..3180)
                     /locus_tag="PLT08_03695"
     CDS             complement(544..3180)
                     /locus_tag="PLT08_03695"
                     /inference="COORDINATES: protein motif:HMM:TIGR01511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042625 - ATPase-coupled ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HNE03596.1"
                     /translation="MTVRDPVCGMEIEPQSAFAEREHMGQTLYFCSQSCVEQFDIDPH
                     HYVMTSATTGFNPERTLTRIELPVADLPFYKPVTALESGLRALEGVHQVTTNAGAGVL
                     QVEYDSKKVNIPQMAAVIRLAGFQPGGSNLKIGIENLRCASCVKFIEDELKSTDGVLS
                     ATVNIATQEASVDYLPQKATLVQLNAAIEAWGYKPRPALTGAPIDKQEEAHAREYIRL
                     MRMFWFSAIVSIPVLLFAYPQYVPGIRDLSMETIRWSWVLSAVATLPVLFYSGYDFFT
                     GAWAAFKHRSANMNTLIALGTGAAWLYSTIAIAFPSVFPEGTSEPFYDVVAVVIALVV
                     LGQALELRAKGQSSAAIKKLLGLQAKTARVIRNGKELDLPVEEVLVGDVIQVRPGEKI
                     PVDGVIVEGSSAVDESMLTGESLPVSKKQGDEVIGATLNKTGAFKFRATKVGKDTALA
                     QIVKMVQDAQNSKAPIARLADTISGYFVPIVMILAVWTFVIWFVIGPQPQLVYALVTS
                     VTVLIIACPCALGLATPMSLMVGIGKGAEHGILIRSGEALQTARAIQTVVLDKTGTIT
                     KGKPELTDVILTNSMNGNSHQLLRLVASVEKVSEHPLAQAIVDGAQARKLELTDVQDF
                     EAIPGHGVSAKVEGRNILIGNLKLMNRENIALGELENRSKSLADDGKTPMFVAIDGKA
                     AGIVAVADTVKEDSAEAIKALQGMGIEVVMITGDNRRTAEAIARKVGVTRVLAEVLPE
                     DKANNVHLLQAENKKVAMVGDGINDAPALAQADVGLAIGTGTDVAIEASDITLIKGSL
                     KGVVTAIEVSRATMTNIYQNLFGAFIYNVLGIPVAMGVLFPFFGLLLSPLIAGAAMAF
                     SSVTVVGNANRLRGFRPKFSVK"
     gene            complement(3314..3667)
                     /locus_tag="PLT08_03700"
     CDS             complement(3314..3667)
                     /locus_tag="PLT08_03700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HNE03597.1"
                     /translation="MKKGNWWIVGIVAVLAALFLFGGGMIWGNRGYGMMGGYGMMGNW
                     GYSPFGWFGMGLGMIFMWLIPIGILVLIGFGVASLVRNTGNPPQASSLTPCSNCGKGT
                     QADWQTCPYCGNSLK"
     gene            complement(3680..3853)
                     /locus_tag="PLT08_03705"
     CDS             complement(3680..3853)
                     /locus_tag="PLT08_03705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HNE03598.1"
                     /translation="MFLMWIVPLVLVGLIVYSVSGNNLVNALKPVASRTCHKCGQAAQ
                     NDWKNCPHCGQTL"
     gene            complement(4007..4186)
                     /locus_tag="PLT08_03710"
     CDS             complement(4007..4186)
                     /locus_tag="PLT08_03710"
                     /inference="COORDINATES: protein motif:HMM:NF016811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YHS domain-containing protein"
                     /protein_id="HNE03599.1"
                     /translation="MTTTVHDPVCHMDIDPATAAGTSEYKGQTYYFCSLGCKKAFDAD
                     PEKYLSKTHEHAHHH"
     gene            complement(4279..4701)
                     /locus_tag="PLT08_03715"
     CDS             complement(4279..4701)
                     /locus_tag="PLT08_03715"
                     /inference="COORDINATES: protein motif:HMM:NF012790.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HNE03600.1"
                     /translation="MKQILLISLFLLVTLTACQSSVEEITGKEVTTVDGSYKNITPVD
                     LDTMLKNTDLILVNVHTPFAGNIADTDLSIPYDQISAPENLAQLPADKNARIVLYCRS
                     GRMSAIAAEELVSLGYTNIWNLEDGMVAWEQTGREIEK"
     gene            complement(4698..5444)
                     /locus_tag="PLT08_03720"
     CDS             complement(4698..5444)
                     /locus_tag="PLT08_03720"
                     /inference="COORDINATES: protein motif:HMM:NF024853.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HNE03601.1"
                     /translation="MDSPINSEDTITFKRTHFYSVLVILAFAIGILTGYVVWGLAPRS
                     QQAIVNNPSVAQGPIVEAPAATQAPQFTRYEIPFEGFPSQGPVDAPIVIVEFSDFQCP
                     FCKRFQDETAAQLLAAYPGQIRFVYRHLPLTSIHPEALPSAEASMCANEQGAFWDYHD
                     RIFENQDKLGRELYLQIAADLNLDAVTFEECLNSGKYKDAIQQDMDFALNLGVQSTPT
                     FFINGLAIVGAQPLEAFKQIIDLELAGKIP"
     gene            complement(5488..5598)
                     /locus_tag="PLT08_03725"
                     /pseudo
     CDS             complement(5488..5598)
                     /locus_tag="PLT08_03725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011173460.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcdA"
     gene            complement(5722..5928)
                     /locus_tag="PLT08_03730"
                     /pseudo
     CDS             complement(5722..5928)
                     /locus_tag="PLT08_03730"
                     /inference="COORDINATES: protein motif:HMM:NF014713.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcdA"
BASE COUNT         1488 a         1541 c         1505 g         1404 t
ORIGIN      
        1 atgtccagaa gttgaccagc accaccttgc cgcgtaaatc ggaaagcgca actgtgctgc
       61 cgtccaacag gtcgagggtg aagttgggcg cagtgaagcc ttctttggca actgcatcct
      121 gctgcgcttt aggctggtcg ggcgagacgc gcgagaaaac agtccagaac attccgagaa
      181 tcaaagccca ggtcatccac gtgataaagc gggagttttt cattatggtg ttccagacag
      241 tgattgcagt ttttcccgca gcatatggac agtgggaaat cccttgacgc cttcaggcgt
      301 ggaatagacg cggcaactca tcgaatacgt ttcgcgttcc tcatgccata aatccatacc
      361 atcaacacga aaatgagggg aaccgaggaa tttcaaacgc tccgcatctt catcggtctc
      421 tactttcacc atttccacag aggcggagat tccctcctct tgtaaagcag tccgaagact
      481 ttccaaacca gcttcccagg agggacaacc atcgaaatag agaagttcaa ttttcataac
      541 agctcacttt acactaaact tcggtctgaa gcctcgcagg cggttggcat tgccaactac
      601 ggtcacggat gagaatgcca ttgctgcccc cgcgatcaat gggctgagca acaatccaaa
      661 gaacgggaac aatactccca tagcaacagg gatacccaac acgttgtata taaaggcgcc
      721 aaataggttt tggtagatgt tggtcatggt agcgcggctg acttcgatgg cagtcaccac
      781 acctttgaga ctgcccttga tcagcgtgat gtccgaggct tcgatggcga catccgtgcc
      841 tgttccgatg gcgagaccga catccgcttg tgcgagcgcg ggggcatcat tgatgccatc
      901 gccaaccatc gcgacttttt tgttttccgc ttgcaacagg tgaacgttgt ttgccttatc
      961 ttcgggcaat acttccgcca gcacgcgcgt gaccccaacc ttgcgagcaa tcgcttcagc
     1021 ggtgcggcga ttatcgccgg tgatcatcac aacttcaatg cccatacctt gcagagcttt
     1081 gatcgcttcg gcagaatctt ccttgaccgt gtctgccacg gcaacaatac cagcggcttt
     1141 gccgtcaatt gcgacgaaca ttggggtctt gccatcgtcg gcgagcgatt tggatctatt
     1201 ctccaactcg cccagcgcga tgttctctcg gttcatcaac ttgagattgc caatcaggat
     1261 atttcgtcct tcaaccttcg cggaaacgcc atgtccaggg atggcttcaa aatcctgcac
     1321 atcggtcaat tccagttttc gcgcctgtgc tccgtccaca atggcttgcg ccaggggatg
     1381 ttcactgacc ttctcaacgg atgccaccag gcgcaacaac tgatggctgt taccattcat
     1441 ggaattggta aggatgacat ccgttaactc aggctttccc tttgtaatcg tgcccgtctt
     1501 atccaacacc acggtttgaa tggctcgcgc agtttgcagg gcttcgccag agcggatgag
     1561 aatcccatgt tccgcacctt tgccaatgcc gaccatcaag ctcatgggcg tggcaagacc
     1621 caaagcgcag gggcaggcaa tgatgagcac ggttacactg gtaacgagtg catataccaa
     1681 ttgcggttga ggtccaatca cgaaccaaat gacgaacgtc cacacggcga gaatcatcac
     1741 gatgggtacg aaataaccag agatggtatc ggcaagacgg gcaattggtg ctttggaatt
     1801 ttgcgcgtcc tgcaccatct tcacgatctg cgcgagagcc gtatccttgc cgaccttcgt
     1861 ggcgcggaat ttgaacgcgc ctgtcttgtt caaggtcgct ccgatcacct catctccctg
     1921 tttcttggaa actggcaacg actcacccgt cagcatcgac tcatccacgg cagaactgcc
     1981 ttcgacgata acgccatcca cgggtatttt ctcaccggga cggacctgga tcacatcgcc
     2041 gaccaacact tcttctacag gcagatcaag ttcttttcca ttgcgaatca cgcgggcagt
     2101 cttcgcttgt agacccaaca atttcttgat cgccgcgctg gattgtccct tggcgcgtag
     2161 ttcaagtgcc tgccccaaca caaccaatgc aatgacaact gcaactacat cgtaaaacgg
     2221 ctcggatgtc ccttcaggaa acacggacgg aaatgcaatg gcaatcgttg aatacaacca
     2281 tgccgcgccg gtaccgagcg cgatcaaggt gttcatgttg gctgagcgat gtttgaaggc
     2341 cgcccacgca cctgtgaaaa agtcataccc ggaatagaac aacacaggta gtgttgcaac
     2401 ggcggaaaga acccatgacc atcggatggt ctccattgac aaatcccgga tgccgggaac
     2461 gtattgcggg tatgcgaata acaacaccgg aatggaaaca attgccgaga accagaacat
     2521 tctcatcagc cggatgtatt cacgggcatg tgcctcttct tgtttgtcta ttggcgcgcc
     2581 agtcagcgca ggacgtggct tgtatcccca agcctcaatg gctgcattca attgtacaag
     2641 ggttgccttt tgggggagat aatccacgct cgcttcctgt gtggcaatgt tgaccgttgc
     2701 gcttaatacg ccatctgtgg acttcagttc atcctcgatg aacttcacac aggaggcaca
     2761 acgcaggttc tcgattccga ttttgagatt cgacccgcca ggttgaaacc ccgccaagcg
     2821 gatgactgcc gccatttgcg gaatgttcac ctttttggaa tcatattcca cctgcaacac
     2881 acccgcacct gcattggtgg ttacttgatg aacgccctcc agggcgcgca aaccagattc
     2941 cagggcggtg acaggtttgt aaaacggcag atcggcgacg ggtagttcga tgcgggtgag
     3001 cgtccgttcg ggattaaaac ccgttgttgc cgaagtcatc acataatggt gcgggtcgat
     3061 atcgaactgt tccacgcagg attgtgagca aaaatacaac gtctgcccca tgtgttcgcg
     3121 ctcggcgaag gcactttggg gttcaatttc cattccacat acagggtcac ggacagtcat
     3181 ggttgcctac cttttataaa gatgccaata gtgtatatcc aatagacgat ggcagggagc
     3241 ggatttcagc ctagtggtat ataagcaaaa tagcctatct tccgacaggc tactttgaga
     3301 acgcgaggtt actttacttc aaggaattgc cgcagtaggg gcaagtctgc caatcggctt
     3361 gggtgccctt tccgcagttg gagcagggcg tcagggatga ggcttgggga ggatttcctg
     3421 tatttcgcac cagggatgcg acaccaaacc caatgagcac gaggatgccg atggggatga
     3481 gccacatgaa gatcatgccc aatcccattc cgaaccagcc gaagggagag tagccccagt
     3541 tacccatcat gccataaccg cccatcatgc catatccacg gttgccccat atcatgccgc
     3601 ctccgaaaag gaacagagca gcaagaacag ctacgatgcc aacgatccac cagttacctt
     3661 ttttcatttt gacttccttt cataaagttt gtccgcagtg cgggcagttt ttccagtcgt
     3721 tttgcgcagc ctgtccacat ttgtggcagg tacggcttgc aacgggcttg agcgcattga
     3781 ccagattatt gccagaaacg ctgtagacga tcaaaccgac aagtactaaa gggacgatcc
     3841 acatgagaaa catactgtac tcctttcgat gatccaagaa tacccggcat gattaaagaa
     3901 tgtgtaaagg cacggcaaag agtgattgaa gattttgttt cggcaaattg tctaaaataa
     3961 aataacctgc ccagtgggca ggttacctga ggagaagagg atggaattaa tgatgatgcg
     4021 cgtgttcatg agtcttgctg agatacttct cgggatcggc atcaaacgcc ttcttgcagc
     4081 caagcgagca aaaataatac gtttgcccct tgtattcgga tgtgccggca gcagtagcgg
     4141 ggtcgatatc catgtggcaa acggggtcat gtacagtggt tgtcataagc tggtctcctt
     4201 tcctttgtat tatgcaatta atagacagcc tcaacgagag ccatattact gaggctctta
     4261 taggcaattc tgcctattct atttctcgat ttcgcgtcct gtctgttccc atgccaccat
     4321 gccgtcttcg agattccaga tattggtata cccgagcgaa acaagttcct ctgccgcaat
     4381 tgcgctcatg cgaccactgc ggcaatacag aacgatcctt gcatttttat ccgctggcaa
     4441 ctgcgccaga ttttccggcg cactgatctg gtcgtaggga atggacaggt ctgtatcggc
     4501 aatgttccct gcgaaaggag tatgcacatt gacaaggata agatccgtgt tcttcaacat
     4561 cgtgtccaaa tcgacaggag tgatgttttt ataggaaccg tcaacggttg taacctcttt
     4621 gcccgtaatc tcttcgacgc tgctctggca ggcggtcagt gtcacaagca gaaacaagga
     4681 tatgaggagt atctgtttca tggaattttt ccagccaact caagatcgat gatctgtttg
     4741 aacgcttcaa gaggttgcgc gccaacgatt gccagcccgt tgatgaaaaa tgttggggtg
     4801 gattgaacgc caaggttgag cgcaaaatcc atatcctgtt ggatggcgtc cttatattta
     4861 cctgaattga gacactcctc gaaagtgact gcatccaaat tgaggtcagc ggcgatctgc
     4921 aggtacaact cacgcccaag tttgtcctga ttctcaaaaa tcctgtcatg gtaatcccag
     4981 aaggccccct gttcgttggc gcacatcgag gcttccgccg atggaagggc ttcgggatga
     5041 atggatgtca ggggaagatg acgatagaca aaacggattt gaccgggata cgccgccagc
     5101 aattgtgcag ccgtctcatc ctggaagcgt ttgcagaacg ggcactgaaa gtcgctgaac
     5161 tcgacaatta cgatgggtgc atccaccggt ccctggctgg gaaagccttc gaatgggatt
     5221 tcataacggg tgaattgcgg tgcctgtgtc gctgccgggg cttcgacaat gggtccctgg
     5281 gcaacagatg gattattgac aatcgcttgt tgtgaacggg gggcaagccc ccagacaaca
     5341 taacccgtca atatgccgat ggcaaaagcc agtatcacca ggaccgaata aaaatgggtt
     5401 cgtttaaagg tgatggtatc ttccgaatta atgggggaat ccataagggt atcttctcta
     5461 aaaatattat gatctttgta tcgtgtgccc gctcagataa ccaagatagg caggcacgag
     5521 cggcaggacg caaggtgaaa gaaatgacaa tagaccggca aggatggcgg tgaagtatgt
     5581 cggcgcggca gagaacaggg taaacttttc gatatataga ccattcttga atgcccagat
     5641 cgctatcaga ctcattgtgt tcgtaagaag cagaacacca atggcaatga tgaaaacacc
     5701 gctggcaatt ttgaaatact tttgataggg tcgcatgcgt ttgatccagc ctgaagcgcg
     5761 ctccaaacca agcgccatcg ccaggaaggg aagccccaaa ccaagcgagt accctgaaga
     5821 tagccacatc gcctgtccga ccgtttgctg gctgagcccc attgttagga ttgcgcccag
     5881 tgtggcacca atgcatgggc tccaacctgc tgcaaagaaa atgcccatta acgccgag
//
LOCUS       DAOQJE010000093         5913 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_7659_length_5913_cov_6.598156, whole genome shotgun sequence.
ACCESSION   DAOQJE010000093 DAOQJE010000000
VERSION     DAOQJE010000093.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 5913)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5913)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5913
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7659_length_5913_cov_6.598156"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1174)
                     /locus_tag="PLT08_03735"
     CDS             complement(<1..1174)
                     /locus_tag="PLT08_03735"
                     /inference="COORDINATES: protein motif:HMM:NF012321.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaJ domain-containing protein"
                     /protein_id="HNE03602.1"
                     /translation="MSASKPDLYSVLGVLRDATQEEIKHAYLAAAQRLHPDKNVAAGE
                     TELFLDVQQAYEVLSNPKRRSQYDSTLPPEVEEKNQFIRHEVLFSRPSLVHLKEDQLL
                     YVLLEVEPTQKQSASLPTPPLNLCLVLDRSTSMQGDKMDILKSTAIQLIRSLRPQDVL
                     SIVTFSDRAEVIVPASISLDKRKQEGNVQMIRPSGGTEIFQGLDAGFKEVRRTLDPAR
                     VNHIILLTDGNTYGDEQDCLQLAEQAAALNIGITGMGIGHEWNDIFLDALAAKTGGSS
                     VYISQPKDIERFLIEKFKALTSVYADEVSLDIRELEHIHLNFAFRIQPEGSQIATTSP
                     LRMGPILRDVPLHVLLEFVVSPEALKGEKVLVLDGLLKASIASNPIPASPIRLKLTR"
     assembly_gap    1213..1312
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1313..1659)
                     /locus_tag="PLT08_03740"
     CDS             complement(<1313..1659)
                     /locus_tag="PLT08_03740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019847115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE03603.1"
                     /translation="MADFFKKLFGSKEESTKPRQVDSATTAPLSDQQLQSILSSQAPR
                     YEIKQLVAAVGQSVGKQRDHNEDSVLSLTNTISGSSESVPFGLYIVADGMGGHQFGEV
                     ASNAAIRIVGGNIT"
     gene            complement(1671..3578)
                     /locus_tag="PLT08_03745"
     CDS             complement(1671..3578)
                     /locus_tag="PLT08_03745"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HNE03604.1"
                     /translation="METRNITSPIGPYGSGSGSGSGDQAGRQQLIPGVTLVNRYSIQD
                     VIGVGGMGSVYRARDMHFPNVVKLVAVKEMINMAPDPLVRQTIVQNFEREANLLATLH
                     HPSIPRIYDYFSHDDRSYLVLEFVQGRDLETVINEANGFLTEDQVISWGIQLCDVLEY
                     LHGHKPDPIIFRDMKPSNVMINQNGDVILVDFGIAKTFQTGIKGTMIGTEGYSPPEQY
                     RGDATPLADVYALGATMHHSLTRRDPRLEPPFTFAERPIRRINSNVSIELEAVVNTAL
                     NYNPTERFPTAASMKEALMNVARKTGTLSKLSAVLPTPASSAIKPIWDFKCEDEIRGT
                     PTLSQGSLYIGCYDNNLYSLNAADGKFQWKYPTDGGIVSRPLVFENNVFFCSEDQRLH
                     VIGARTGKLLWTYYTEGKIYSSPRISDGHVFFGSEDHDLHAVNVNSGRAVWKFSTDGP
                     IYSTPLVANEMIYFGAESGSFYGLDFRGAMKWRFAAKRGMTSSPISKGQAIYTASLDG
                     TLYALDAKSGWAIWKFRLGKGSVSSPVIADDFIFLGAADGFVYCVDIRTAKEVWRYKT
                     DNQVSSSPTIYKDSIYIGSVDGSMYCLEYRTGRLRWKFETGGPITGSPVVLDDIVYFG
                     STDHHVYALFA"
     gene            3703..4326
                     /locus_tag="PLT08_03750"
     CDS             3703..4326
                     /locus_tag="PLT08_03750"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HNE03605.1"
                     /translation="MKILAVSDQVVERVYSLAPSGHFKGVELILGCGDMPYTYLEYLV
                     TILSVPLFYVPGNHDPDFNPRDHRSRAEGGSNLDLRLVYHNKSLIGGFGGSIRYRPDG
                     TNQYTQNEAFLRAFNMVPKLVMNRIKYGRALDILISHSPPFGIHDDTDYAHNGLKALN
                     WLLDFAKPRYHFHGHTHFYRNNIEQSITKTSTTQIMNVYPYKVVDIE"
     gene            4421..5269
                     /locus_tag="PLT08_03755"
     CDS             4421..5269
                     /locus_tag="PLT08_03755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE03606.1"
                     /translation="MSDVIDSKIRADFSRARFKSFINQVFSVLSGQRNTLLSYDDIKE
                     KLRIGGPIYRGMKTVRVEQIAGSLNRYHEFDRAFLPKEDQLASRWQKVDRAFYEDIHL
                     PPVVLYKVGQVYFVVDGHHRVSVAREQGQEYIEAEVRECATRVNITPDIRPEDLEVLG
                     AKVDFLERTGLDKYRPDANIKLTIPDGFTRMLEHIAVHRYFMGLDFKRDISEQEAVEH
                     WYDKVYVPIVSIIRESGLLDEFPDKTESDLYLWALDHQHYLYQEEGQPLQPPELAAKQ
                     FILENE"
     gene            5342..>5913
                     /locus_tag="PLT08_03760"
     CDS             5342..>5913
                     /locus_tag="PLT08_03760"
                     /EC_number="4.1.3.3"
                     /EC_number="4.2.1.41"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: protein motif:HMM:NF012904.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009436 - glyoxylate catabolic process
                     [Evidence IEA];
                     GO_process: GO:0071704 - organic substance metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HNE03607.1"
                     /translation="MTPLHPLAGVYAAAVTPYLGAPKTAGKPDTAFDFESLAMFLHFL
                     AGRGCHGALLFGTTGEGPSFSPREREALLRSIRVIKHQVRDFKFLVGTGTPSLTETIE
                     LTKLAYDLGYDGTVVLPPYYFRKANDEGLFRWFSELIENAVPQGKHLLGYHIPVMTGV
                     GFSLDLLERLKTKYPNQFAGIKDSSHDEAFA"
BASE COUNT         1611 a         1336 c         1341 g         1525 t
ORIGIN      
        1 gacgggtcag tttgaggcgg atgggagatg cagggatagg attggaagca atagatgctt
       61 ttaggagtcc atccaatact aagaccttct cccctttcaa tgcttcgggg ctgacaacaa
      121 attccaacaa gacatgcaaa ggcacatcac gcaagattgg tcccattcgc aaaggcgaag
      181 tggttgcaat ttggctgccc tccggctgga tgcgaaacgc aaagttaagg tgaatgtgct
      241 caagttctct gatatccagc gacacttcat cagcgtaaac actggtcaac gctttgaatt
      301 tttcgattag gaagcgttca atatccttcg gctgggatat ataaacggaa gatccgccgg
      361 tttttgcagc aagagcatct aaaaagatgt cattccattc atgcccaatc cccatgccgg
      421 taataccaat attcagggca gcagcttgct ctgcaagttg caagcaatct tgctcatcgc
      481 cgtaggtgtt tccatcggta agaagaataa tgtgattgac acgtgctggg tctagagttc
      541 ggcgaacttc tttgaacccg gcatctaatc cctgaaagat ttccgtccca ccagaaggac
      601 ggatcatttg cacattgcct tcctgtttgc gtttgtctag gctgatcgaa gcagggacga
      661 taacttcagc acggtcacta aaggtgacga tgcttagcac atcctgcggt cttaaactac
      721 ggattagctg aattgctgta gacttcaaga tatccatctt atcgccttgc atggaggtag
      781 atcggtctaa caccaagcaa aggtttaatg gaggcgtagg taggctggca gattgttttt
      841 gtgtaggttc aacttccaac agcacataca ataattggtc ctcttttaag tgaaccagac
      901 taggacggct gaaaaggacc tcgtgacgaa taaattgatt tttttcttca acttcaggtg
      961 ggagcgtaga gtcgtattga ctgcggcgtt tggggttcga gagaacttca tacgcctgtt
     1021 gaacatcaag gaagagttca gtctcgccag ctgctacgtt tttatctgga tgcagccgtt
     1081 gagcggcagc caaataggca tgcttaatct cctcctgtgt ggcatcacgt aacacaccta
     1141 gaacggagta tagatccggc ttgctggcag acatgagagg gtttatccaa taagttgagc
     1201 gagaatgacc gtnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1261 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nngtgatatt
     1321 gccaccgaca atgcgaatag cagcattgct tgctacttca ccaaattgat gtccacccat
     1381 accatcggca acgatatata agccaaatgg tacactctca gaactacctg aaatggtgtt
     1441 cgtcagggaa aggacactat cctcattgtg gtcacgttgt ttccctacag actgacctac
     1501 agccgcaaca agttgcttga tctcatagcg aggtgcctgg ctggataaaa tggactggag
     1561 ttgttggtca gaaagcggag ctgttgtagc agaatccact tgtcttggtt ttgttgattc
     1621 ctctttggaa ccaaaaagtt tcttgaaaaa atcagccaca agttctcctt ttaggcaaac
     1681 aacgcataaa cgtgatggtc ggttgagcca aaataaacaa tatcatctaa aacaacgggg
     1741 gaaccggtga tgggtccacc tgtttcaaat ttccaacgta aacgccctgt acgatattca
     1801 agacagtaca tggaaccatc cacagagcct atgtagatag agtctttata gatggttggt
     1861 gaactgctca cctgattatc cgttttatag cgccaaactt ctttggcagt gcgaatatca
     1921 acacagtata caaagccatc cgctgcaccc aggaagataa aatcgtccgc aataaccggc
     1981 gaagagactg accccttccc taaacggaat ttccagattg cccagccgct ttttgcgtcg
     2041 agggcataca aggttccatc caaagaggct gtataaattg cctgtccctt actgattggt
     2101 gaagacgtca tgccacgttt agcggcaaaa cgccacttca tggcaccacg gaaatctagc
     2161 ccgtaaaacg aaccagactc tgcgccaaag tagatcattt catttgccac aagcggagta
     2221 gagtaaatag gtccatcggt agaaaacttc cataccgctc taccactatt cacgttcaca
     2281 gcgtgaaggt catgatcttc ggagccgaaa aagacatgcc cgtctgagat gcgtggagaa
     2341 gaataaatct ttccttctgt gtaataagtc cacagtaatt tgcctgtgcg ggcaccaatt
     2401 acatgcaagc gttgatcttc agaacaaaag aagacattat tctcaaaaac caatgggcgg
     2461 gaaacgatgc cgccatcagt cgggtatttc cactggaact ttccatcagc agcgttcagg
     2521 gaataaaggt tattgtcata acagccaata tacaaagatc cctgactcaa tgttggtgtg
     2581 ccgcgaattt catcttcaca cttaaaatcc caaataggct tgatcgcact actggcaggg
     2641 gtcggcaaaa ccgcagaaag ctttgaaagg gttcctgtct ttcgagccac attcatcaaa
     2701 gcttccttca tagatgctgc tgttgggaag cgttcagtag ggttgtaatt caacgccgta
     2761 tttaccacag cttctaactc aatggaaaca ttcgagttaa tgcggcggat tgggcgttcg
     2821 gcaaaagtaa aaggaggttc gaggcgtggg tcgcgtcggg taagagaatg atgcatggtg
     2881 gcacccagcg cgtacacatc cgcaagaggc gtggcatccc cacgatattg ctcaggtggc
     2941 gaatatcctt ccgttccgat catcgtgcct ttaataccgg tctggaatgt cttggcgata
     3001 ccgaagtcaa ccaaaatgac atcgccattc tggttgatca tcacgttcga gggtttcata
     3061 tcacgaaaaa tgatgggatc tggcttatga ccgtgcaaat attccaacac atcacaaagc
     3121 tggattcccc agctaattac ctgatcttct gtcagaaagc catttgcttc attgataacc
     3181 gtttcaaggt ctctaccttg aacaaactct aaaacaagat aagaacgatc atcatgggag
     3241 aaataatcat aaatacgtgg aatagatgga tgatgcagag tagccaacaa attggcttca
     3301 cgctcaaagt tctgtacgat cgtctggcga accaaggggt ctggtgccat attgatcatt
     3361 tctttgacag ctaccagctt aaccacattc gggaaatgca tatcccgagc gcgatacaca
     3421 gaccccatac caccaacgcc tataacatct tgaatgctat agcggttgac aagggtcacg
     3481 cctggtatta attgctgacg tccggcttga tcaccggagc ctgagccgga acccgagcca
     3541 taaggaccaa tgggagaggt aatgtttctc gtttccatct gtgttttttt ccaaaaattt
     3601 aatgaattat aagggtattt gccgatgctg gctattgcaa ttttgcaaaa agctcacttt
     3661 tggggttgca atcgagcgtt ttctacggca caatgagccc ctgtgaagat attggcagta
     3721 agtgatcagg tggtagagcg tgtctactcg ctggcgccga gcggacattt caaaggtgtt
     3781 gaactgattc ttgggtgtgg agacatgccc tacacctacc ttgaatacct cgtcactatt
     3841 ttgagtgtgc cactttttta tgttccgggg aatcatgacc cagacttcaa cccgcgtgac
     3901 caccgttctc gagcagaagg cggctcaaac ctagacttga gactggtcta ccacaacaag
     3961 agcttgatcg gcggcttcgg cggttccatc cgctacagac ccgatggcac gaatcaatat
     4021 acacagaatg aagctttctt acgggcattc aatatggtac ccaaattggt catgaatcgt
     4081 atcaagtatg ggcgcgcact ggatattctc attagccatt ctcccccatt tgggatccat
     4141 gatgatacag attatgccca taatgggtta aaagcattaa attggctgtt agattttgca
     4201 aaaccacgtt atcattttca tggtcatacg cacttttatc ggaataatat cgagcagagc
     4261 ataacaaaaa ctagcactac tcagatcatg aacgtctatc cttacaaggt tgtcgatatt
     4321 gaataagaca tatcctaatc ggatattaat ctcacaataa cttatttatt agtatgtttt
     4381 atataataga ctgaattgtt attagcaaag agagaaagaa atgtcagacg taatagattc
     4441 aaaaatacgt gcagatttta gtcgggcacg tttcaaatcc ttcatcaatc aggtattctc
     4501 tgtcttaagc gggcagcgaa atactttgct ttcttatgat gatataaagg aaaaactccg
     4561 tatcggggga cctatctatc ggggtatgaa aactgtccgc gtggaacaga ttgcggggag
     4621 tctgaaccgg taccatgaat tcgaccgagc cttcttaccc aaggaagacc aacttgccag
     4681 ccgctggcaa aaagtcgatc gtgcttttta tgaagatatt catttaccac ctgtggttct
     4741 atataaagtg ggtcaggtgt acttcgttgt ggatggacat catcgtgtgt cggttgctcg
     4801 tgagcaagga caggaatata tcgaagctga agtgcgagaa tgtgcaactc gggtcaacat
     4861 cacgccagat attcgtcctg aagaccttga agttctcggt gccaaagtgg attttcttga
     4921 gcgtaccggt ttggacaaat accgacccga tgcaaatatc aaactgacga tccctgatgg
     4981 gttcacacgt atgcttgaac atatcgccgt ccatcgttat tttatggggc ttgatttcaa
     5041 acgcgatatt tcagaacaag aagctgtgga acattggtac gacaaggtgt acgtccctat
     5101 cgtatccatt attcgtgaaa gcggactctt ggatgaattt ccagataaga ctgaaagcga
     5161 cctatatctc tgggcgttag accatcagca ttatctatat caggaagaag ggcaaccact
     5221 tcaaccgccg gaattagcag ctaaacaatt tattctcgag aacgagtaaa ctagtaggga
     5281 cgaccccaaa gaaagatttt gaaaggtcgt ccctacttta ttttggagac aaagttcatc
     5341 tatgacaccc cttcatccat tagcaggtgt ttatgctgct gcagttaccc cctacctcgg
     5401 tgcacctaaa acagccggga aaccagacac ggcttttgac tttgagtcac tggcgatgtt
     5461 tcttcacttc ctcgccgggc gcggatgtca cggcgcgttg ctattcggaa ccacaggcga
     5521 aggtccgtcg ttctcgccgc gcgaacgtga agctctgctt cgttccattc gcgtcattaa
     5581 acatcaggtg cgtgacttta agtttttggt cggcaccgga acacccagcc tgactgaaac
     5641 catcgaactg accaaacttg cttatgacct tggttatgac ggaactgtgg ttcttccgcc
     5701 gtattacttc cgcaaagcca atgatgaggg attgttccgt tggttcagcg aattgatcga
     5761 aaatgctgta ccgcaaggaa agcatctgct cggctatcac atcccggtaa tgaccggtgt
     5821 gggcttttcg cttgacctgc ttgaaaggct caaaacgaaa tatccgaacc aatttgcagg
     5881 tatcaaagat tcatcacatg atgaagcctt tgc
//
LOCUS       DAOQJE010000094         5819 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_7859_length_5819_cov_6.313151, whole genome shotgun sequence.
ACCESSION   DAOQJE010000094 DAOQJE010000000
VERSION     DAOQJE010000094.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 5819)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5819)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5819
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7859_length_5819_cov_6.313151"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..102
                     /locus_tag="PLT08_03765"
     CDS             <1..102
                     /locus_tag="PLT08_03765"
                     /inference="COORDINATES: protein motif:HMM:NF012412.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE03608.1"
                     /translation="DEVADGPHSVIFQQAHNRLHAQKAILARLFDVA"
     gene            136..1338
                     /gene="rocD"
                     /locus_tag="PLT08_03770"
     CDS             136..1338
                     /gene="rocD"
                     /locus_tag="PLT08_03770"
                     /EC_number="2.6.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004587 - ornithine-oxo-acid transaminase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine--oxo-acid transaminase"
                     /protein_id="HNE03609.1"
                     /translation="MNTQDFIKIEEQYGAHNYHPLDVVIEKAEGVWVYDVEGRKYLDC
                     LSAYSAVNQGHVHPEILNALLEQAKKVTLTSRAFRNDQLPLLYKELSEMTGYDMSLPM
                     NSGAEAVETAVKLARKWAYQVKKVPRHQAEIIVAGNNFHGRTVTIVSFSTEPLYRDDF
                     GPFTPGFVVVEYGNLDALEKAITPNTAAVLLEPIQGEAGVIMPPAGYLKKVAELCKKN
                     NVLYISDEIQTGLGRTGKLFAANHEDVRPDVVIVGKALAGGFYPVSAVLADKPILGLF
                     TPGEHGSTFGGNPLAAAVARASLKVLREEKLADRAAELGTYFVEQLSEIPSPHVKEVR
                     GKGLLIGVELKPEAGGARRFCEALKSKGILAKETHDNVIRFAPPLVIDKDTIDWALPA
                     IRETLNMP"
     gene            1397..2488
                     /locus_tag="PLT08_03775"
     CDS             1397..2488
                     /locus_tag="PLT08_03775"
                     /inference="COORDINATES: protein motif:HMM:NF013430.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine dehydrogenase"
                     /protein_id="HNE03610.1"
                     /translation="MYIGIPKESRPFEYRVGLSPAGVEILSHLGHQVFVEHDAGASAG
                     FKDQDYEKAGGRIAFSAQEVFGRADFLLKVSRPIKQEMEWINDGAILAGFLHLASASQ
                     DQIDLLLEKKITALAYEQITDAQGGRAVLRPFSLICGAMVAQIAARWLQTNRGGKGIM
                     LSGVPGVPPAEVVIIGGGAVGTSAAQAMQNAGAHVTVLDKNMAALENIHDKLPGVVTM
                     MATQRNIERATAYADVVVGAVLVAGQRAPMVITREMVRAMKPRSVIIDVSIDQGGCVE
                     TSRPTMHDNPTFIEEGVVHYCVPNIASTVARTASHVFLNSAIPYITAIANTGIDAAMK
                     NDPSIEAAVNTHQGKLVNLVRLGGVEVKE"
     gene            2485..3795
                     /locus_tag="PLT08_03780"
     CDS             2485..3795
                     /locus_tag="PLT08_03780"
                     /inference="COORDINATES: protein motif:HMM:NF014594.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA hydrolase/transferase C-terminal
                     domain-containing protein"
                     /protein_id="HNE03611.1"
                     /translation="MNPSSIYQSRVTTAEEAIKQVKSGNRVFLTGNVSVPKVLLSALV
                     GHAPNINDVEVCQALTIGAADYVTEAMEGHLRVNSMFISANVRKAVQDGRADFTPVLL
                     SEFPLLFKRGILPVDVALIHVSRPDEHGFCSLGVEVGLTKSAAESAKIIIAEVNDQMP
                     RTLGDSFIHVSRLNYIVPVNYPIPEHKMAAEGDSDIVQGIAQHVASLIPDGATMQLGI
                     GSIPDAVLKFLMDKKDLGIHTELFSDGVIELVNAGVLTNARKSLHPGKIVAGFILGTQ
                     KLYDWVNDNPMVEMHPTEYVNDPFIIMQNERMVAVNSAIEVDLTGQVCADSIGPKLYS
                     GVGGQLDFIYGASRSKGGVPIIALPSTATMRDGTKVSKITAMLKPGAGVVTSRNHVHY
                     VVTEYGIADLYGKTIRQRAQALIKVANPIFHDELEKQAKELHYL"
     gene            4005..5477
                     /gene="pyk"
                     /locus_tag="PLT08_03785"
     CDS             4005..5477
                     /gene="pyk"
                     /locus_tag="PLT08_03785"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: protein motif:HMM:NF004978.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HNE03612.1"
                     /translation="MRNAKIVATIGPASDSEQALESLIKAGMNVARLNFSHGTHEQHQ
                     VRVKRIREVSERLNMPVGILQDLQGPKIRVGKLDVPLQLAVNEEVCLYATNDTPPQNG
                     CQLIPVDFRELFESVQKGDKLLLDDGRLALQVTSAEGRQVHATVKVGGALSSHKGINL
                     PGVKLRIAGFTSKDKADLAFGISQGVDAVAISFVRSADDVREVRAAIKEFSAGKHEPL
                     LIAKLEKPEAMVELEEILEVVDGVMVARGDLGVELPPEQVPPLQKMIIRAANARAKLV
                     ITATQMLESMIQNPLPTRAEASDVANAVFDGTDAVMLSAETASGEYPSLAVEMMSRIV
                     SEAESHFKEWGSRHDSKAGLGESDAASMARASNVLASDPEVQAVSVFTMRGRSAWLMS
                     KARPSKQILAFTPERETFNQLAFLWGVRPHLVKFAKTMDDMLSEVDSVLLKSGISSGQ
                     QVVLVCGFPIGEQRPPNMALLHTVASDPNLSLARKLAEKN"
     gene            complement(5557..>5819)
                     /locus_tag="PLT08_03790"
     CDS             complement(5557..>5819)
                     /locus_tag="PLT08_03790"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HNE03613.1"
                     /translation="TQGVGEITLGIIGGLLFLGFISTALAMFLWNYAFAELPAAVASL
                     TFFAQPVVGTLLGWFFLSEQITSLFIIGGVLIGVGIIIASRE"
BASE COUNT         1436 a         1356 c         1569 g         1458 t
ORIGIN      
        1 gacgaagtag cggatggtcc acactcggtg atcttccagc aggcgcataa tcgcttgcac
       61 gcgcagaagg caattttggc gcgtttattc gatgtcgcat aaagatattc atagttttta
      121 gaggataccc attttatgaa tacccaagac tttatcaaga tcgaagaaca atacggcgcg
      181 cataattacc atccattgga tgtggtcatc gaaaaagcag aaggggtgtg ggtttacgat
      241 gtggaaggca gaaaatacct tgattgtctt tctgcatatt ctgctgtaaa ccaagggcat
      301 gttcatcctg aaattttgaa tgcattgttg gaacaggcaa agaaggtcac actcacttcg
      361 cgcgctttcc gcaatgacca gttgccgctt ctttataaag aactttctga aatgaccggc
      421 tatgatatgt ctctgccgat gaactctggt gcagaagcag ttgagacggc ggtgaagctt
      481 gctcgcaagt gggcgtatca ggtcaagaag gtgccgcgcc atcaggcgga aattatcgtg
      541 gcgggcaaca acttccacgg gcgcaccgtc accattgttt cattctccac agagccactc
      601 tatcgggatg atttcggtcc gttcacgccg ggttttgtgg tggttgaata tggaaacctt
      661 gatgcgcttg aaaaggcaat tacgcccaac acggctgcgg ttttgctcga accgattcag
      721 ggagaggcag gggtgatcat gccgccggct ggatacttga agaaagttgc cgagctttgc
      781 aagaagaata atgtgcttta tatctcggat gaaattcaaa cgggtctcgg tcggaccggg
      841 aaactgtttg ccgcgaatca tgaagacgtc cgcccggatg ttgtgatcgt aggaaaggca
      901 ttggctggtg gcttttaccc cgtctctgct gttctcgcgg ataaacccat tttgggcttg
      961 tttacacccg gtgaacacgg ctccacattt ggtggaaatc cgctcgctgc tgcggtggct
     1021 cgtgcttcat tgaaagtttt gcgcgaagaa aaattagcag accgtgcggc agagttggga
     1081 acttattttg tagagcaatt gagcgaaatc cccagtccac atgtcaagga agtgcgcggt
     1141 aagggcttgc tcattggtgt ggaactcaag ccagaagcag gcggagcccg tcgtttctgt
     1201 gaagcattga agagcaaggg catacttgct aaagaaacgc atgataatgt gatacgtttt
     1261 gcaccaccac ttgttattga taaagatacg atagattggg cgctccctgc gatcagggaa
     1321 actctcaata tgccgtaata aattaaacca gaccctaaag gtttatatac ctctagggtc
     1381 tttttatgga ggaaccatgt atattggaat tccgaaagaa agtagaccgt ttgagtaccg
     1441 agttgggctg tctccggctg gggtggagat tctgtctcat ttggggcatc aagttttcgt
     1501 tgagcatgat gcgggggcga gtgctggctt caaagaccag gattacgaaa aagccggcgg
     1561 gcgcatcgcc ttttctgcgc aagaagtttt tgggcgggca gactttttat tgaaggtctc
     1621 acgcccgatc aagcaggaaa tggaatggat caatgatgga gctatccttg cggggttttt
     1681 acatctcgct tctgcttcac aagatcagat tgacctgctc ttggagaaaa agattacagc
     1741 gctggcatac gagcagatta cagatgctca aggcggacga gccgttttgc gtccgtttag
     1801 tttgatctgc ggtgcgatgg tcgctcaaat tgcggcgcgt tggctgcaga cgaatcgcgg
     1861 tggcaagggc attatgctca gtggcgtacc cggtgtgccg cctgccgagg tggtaattat
     1921 cggtggtggt gcggtgggaa cttctgccgc gcaagccatg caaaatgccg gtgcgcatgt
     1981 gactgtccta gataagaata tggctgcgtt ggaaaacatc cacgataagt tgcctggtgt
     2041 ggtaaccatg atggcaacgc aacgcaacat tgaacgtgcc acagcctatg cggacgtagt
     2101 tgtgggggcg gttttggtgg caggtcaacg cgcaccgatg gtgatcacac gcgaaatggt
     2161 ccgtgctatg aaaccgcgct cggtcattat tgatgtcagt attgatcaag gtggttgtgt
     2221 agaaacctct cgcccgacca tgcatgacaa ccccactttc attgaggaag gtgttgtgca
     2281 ttactgtgtt cctaatattg ccagcacagt ggcgcgcaca gccagccatg tgtttttgaa
     2341 ctcagctatt ccctacatca cagcaatagc gaacacaggc attgacgccg ccatgaagaa
     2401 tgacccttca attgaggctg cggtcaatac acatcaaggg aagttagtca accttgtacg
     2461 tttgggtggc gtggaggtga aggaatgaat ccatcttcca tttatcaatc ccgtgtgacg
     2521 actgcagagg aagccatcaa gcaggtcaaa tcgggcaacc gcgtctttct gaccgggaac
     2581 gtctcggtac ctaaggtgct tttgtctgca ctggtgggtc atgctcccaa tatcaatgac
     2641 gtggaagttt gccaggcatt gacgattggc gctgccgatt acgtaactga agccatggaa
     2701 gggcatttac gagtcaattc catgtttatt agcgccaatg ttcgtaaagc agtccaagat
     2761 ggtcgcgcag actttacgcc tgtattactt tcagaattcc cacttctatt caaacgtggc
     2821 attctgcctg tggatgtggc actgattcat gtctctcgcc cagatgaaca tgggttctgc
     2881 agccttggcg tggaagtggg gcttaccaag tctgcggctg agtctgccaa gatcattatt
     2941 gctgaagtca atgatcagat gccgcgtaca ttaggggatt ccttcattca tgtgagccgt
     3001 ttgaactaca ttgtgccggt caattacccc atccctgaac ataagatggc agccgaaggt
     3061 gactcggata tcgtccaagg cattgcccag catgttgctt cgctgattcc cgatggcgcg
     3121 accatgcaac tgggtattgg ttccatccct gatgctgtgt tgaaatttct gatggataag
     3181 aaagatctcg ggattcatac cgagttattc tcagacggcg tgatcgaatt ggtcaatgca
     3241 ggagtgttga ccaatgctcg caaatctctg cacccgggca agattgtggc tggttttatt
     3301 ttgggcaccc aaaaacttta tgattgggtc aacgataacc caatggtcga aatgcacccg
     3361 acggagtatg tgaatgatcc gtttatcatt atgcagaatg aacgcatggt ggcggtcaac
     3421 tctgccatag aagtggacct tactgggcaa gtctgtgccg atagcattgg tccgaagcta
     3481 tacagcggtg tgggcggaca gttagatttc atttatgggg cgtcccgctc caaaggtggt
     3541 gtgcccatca ttgctttgcc tagcaccgca accatgcgag atggcaccaa ggtgagtaag
     3601 atcactgcca tgctaaaacc aggtgccggc gtggtcacga gccgtaatca tgtccattat
     3661 gtggtgacag agtatggcat cgcggactta tacggcaaga ctatccgcca gcgggctcag
     3721 gcactgatca aggtagcgaa cccgattttc cacgatgagc tggaaaagca ggcgaaagaa
     3781 ctgcattacc tttaaaagag tacccaaaaa gggcatggag tttgcgtaac tccatgccct
     3841 ttttattgtt aaacccataa taatttgccc cggcttgaca ttcctctgtt ttagacctat
     3901 catacggggc gttttgagac acaaacaccc cgtatttttt aacgggtttg cgatggattt
     3961 tattttcaac gcagaacaaa tcattattga caggaagcag cactatgcgt aacgcaaaaa
     4021 ttgtagcaac gatcggtccc gcctcagatt cagagcaggc gttggaatcc ttgatcaagg
     4081 cgggcatgaa tgtcgcccgg ttaaacttct cccacggcac ccatgaacag caccaagttc
     4141 gtgtgaagcg aattcgagaa gtatcagaac ggcttaatat gccagttggc attttgcaag
     4201 atttgcaggg tcccaagatc cgtgtgggta aattagatgt tcctcttcag cttgcggtaa
     4261 atgaagaggt ttgcttgtat gccactaacg atactccgcc acagaacggc tgccaattga
     4321 tccctgtgga cttccgtgag ctgttcgagt ctgtacagaa gggcgataag cttcttttgg
     4381 atgacgggcg cctcgcattg caagtcactt cggcagaagg caggcaagtt catgctactg
     4441 tgaaagttgg tggggcatta tcttcgcaca aaggcatcaa cttgcccggt gtcaaacttc
     4501 gcattgcagg ctttacttca aaagataaag cggaccttgc ttttggtatc tcccaaggcg
     4561 tggatgcggt tgcgatttct ttcgtccgca gcgccgatga tgtccgtgaa gtccgcgcgg
     4621 cgattaagga attttcagcc ggtaaacatg aaccgttgtt gatcgccaag ttagaaaagc
     4681 ctgaagcgat ggttgagttg gaagaaatcc ttgaggttgt agacggtgta atggtagcgc
     4741 gtggcgattt gggcgtggaa ctcccgccgg aacaagtgcc gcctctgcaa aaaatgatca
     4801 ttcgcgcggc aaatgcccgt gccaaactgg tgattacggc gactcagatg cttgagtcga
     4861 tgatccagaa tccgctgccg actcgtgctg aagcttcaga cgttgccaat gccgttttcg
     4921 atggcaccga tgctgtcatg ctctccgctg aaactgcttc gggcgaatac cccagccttg
     4981 cggtggaaat gatgtctcgt attgtgtcag aagcggagtc tcacttcaaa gagtggggca
     5041 gccgtcatga ctcaaaagcc ggcttaggcg aatctgatgc ggcgtcgatg gcgcgtgcat
     5101 ccaacgtatt ggcaagcgac cccgaagtgc aggcggtctc tgtcttcacc atgcgcggac
     5161 gttccgcgtg gctcatgtcg aaggcgcgcc cgtcgaagca aattcttgcc tttacacccg
     5221 agcgcgaaac cttcaatcaa ctggcgttct tgtggggcgt tcgtccgcat cttgtaaagt
     5281 ttgccaagac gatggacgat atgctctctg aagtagactc agttctgttg aaatctggca
     5341 ttagttctgg gcagcaggtg gtgttggttt gcggtttccc cattggcgaa caacgcccac
     5401 ccaatatggc gttgcttcac accgtagcga gtgatccgaa tctctcgctg gcgcgcaaac
     5461 tggcagagaa aaattaaatt agcaaacaag aagcggatgc aacaaagtgc atccgcttct
     5521 ttcttaaccg aggcttgctt cagcgcttca ggcttttcat tccctcgaag cgataataat
     5581 cccgaccccg atcaataccc caccgatgat aaataatgac gtgatctgtt cggagagaaa
     5641 gaaccagccg agcaaagtgc caacgacagg ctgggcaaaa aaggttagtg aagcaactgc
     5701 cgcaggcaat tcggcgaagg cgtaattcca taagaacatg gcaagcgcag ttgagataaa
     5761 cccaaggaat aacaatccgc caatgatgcc aagtgtgatc tcgccaacac cttgcgtat
//
LOCUS       DAOQJE010000095         5810 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_7886_length_5810_cov_5.998089, whole genome shotgun sequence.
ACCESSION   DAOQJE010000095 DAOQJE010000000
VERSION     DAOQJE010000095.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 5810)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5810)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5810
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7886_length_5810_cov_5.998089"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..221)
                     /locus_tag="PLT08_03795"
     CDS             complement(<1..221)
                     /locus_tag="PLT08_03795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE03614.1"
                     /translation="MKSKTVRFFAGILVLLILQGCNLPSAVQPIETALPSTEAPATEL
                     QPVTPSEIQHQNIPISAPVAKPYPDVTSK"
     gene            complement(342..3182)
                     /gene="ppdK"
                     /locus_tag="PLT08_03800"
     CDS             complement(342..3182)
                     /gene="ppdK"
                     /locus_tag="PLT08_03800"
                     /EC_number="2.7.9.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050242 - pyruvate, phosphate dikinase
                     activity [Evidence IEA];
                     GO_process: GO:0015976 - carbon utilization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate, phosphate dikinase"
                     /protein_id="HNE03615.1"
                     /translation="MSKWVYLYNEVKEVEKLAKGSWDGVKAIVGGKGAGLMDMTRANV
                     PVPPFFTVTTEACNAYQKAGKFPQGMWEQELKALKEIERKTGKKFGDPKNPLLVSCRS
                     GAKFSMPGMMNTILNIGLTDIVAEGMIKATNNDRFVWDSYRRVVEMFGTVVMDIEDEA
                     FEHPLEAYKEKKGYKLDIEMRAEDWKEVVGIYKQAYKKACGQEFPQDPYKQLSLATEA
                     VFRSWNGKRAVAYRRKEGISDALGTAVNICTMVFGNMGDDSATGVAFTRNPSTGEKKM
                     MGEYLLNAQGEDVVAGIRNTDAIENLKKYMPKAYAQFMEITARLEKHYTDMQDVEFTI
                     ERGKLWMLQTRVGKRTAKAAVKIAVDMANEGLINKDQAVQRVTPDQVDALLHPQFNDE
                     AMNQAEKEGKFLAKGVNASPGAAVGQIYFDADKAEEYAKEHKQDTIMVRPFTKPDDVH
                     GMIAAKGVLTSEGGATSHAAVVARQFGIPCVVGATAIKIDLEKRIMHVGEIVLNEGDW
                     VSVDGTTGKVFAGKIPTSEPSLEEQKELMTLLKWADDISARKNIREEVEPGYPTRGLQ
                     VWANADYPKDARRARSYGAVGIGLCRTEHMFFEPERLPIVQRMILAETSEGRTEALNL
                     LLPHQRKDFDGLFEAMDGYPVIIRLIDPPLHEFMPDEEKLLEEVITMRVKGETKDLAE
                     KEKLLESIKGMHESNPMMGLRGVRLSISMPEIVEMQVRAIFEAAADCTLRGIRVKPEI
                     MIPLTGTVKELDWIQPRLERIGKAVMQEKKIKFAYKFGSMIEIPRAAVTADHIATQAQ
                     FFSFGTNDLTQMTFGYSRDDAERNFLITYVQEGILVKNPFQTLDRNGVGKLMQRAIFE
                     GRAVRPDLEVGICGEHGGDPDSIEWCHLIGNNYVSCSPFRVPVARLAAAHAALKHQPA
                     KKAKVAKKPAAKKVVKKVVKKAAKKVAKKSKK"
     gene            3386..3829
                     /locus_tag="PLT08_03805"
     CDS             3386..3829
                     /locus_tag="PLT08_03805"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HNE03616.1"
                     /translation="MTPTAKILVIDDDAAITELMSMLLKTFGFEVLTTNIGEEGIRLA
                     REANPNVILLDLMMPDLDGWQVCKEIRKTSTVPILILSAINDPRMVASVLDVGADDFL
                     VKPVPSGVLVANIKKLIRRNTGALDMSKLKKTTASLQQANHALHP"
     gene            complement(3893..4657)
                     /locus_tag="PLT08_03810"
     CDS             complement(3893..4657)
                     /locus_tag="PLT08_03810"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HNE03617.1"
                     /translation="MNKIKLNGIELAYERRGMGTPLMLIHGYPLDHSIWNDVAAQLEN
                     DFDLIIPDVRGFGKSTTVDTPYSLSDIANDLAALLDSLGVGPTALAGHSMGGYIALAF
                     AKSFPQRVTGLALVSSQAASDTPERKEGRYKTAADVVQKGVQIVADAMTDKLSPNQSV
                     RDFVRPLIAKQSVAGVTGALKAMAERDDMTSFIASYAQPLVLIHGDADELIPLERARE
                     IKSLVSSAQLVELAGVGHMPMLEMPNETASALKKLK"
     gene            complement(4664..5200)
                     /locus_tag="PLT08_03815"
     CDS             complement(4664..5200)
                     /locus_tag="PLT08_03815"
                     /inference="COORDINATES: protein motif:HMM:NF024873.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease H-like domain-containing protein"
                     /protein_id="HNE03618.1"
                     /translation="MNYVFFDLESQFLFDEVGGRDHADKLKVACAVTYSTAKNDFTVY
                     WENDVPALLAELKAATKVIGFNLISFDYRVLQPYANQVRLASIPTLDMLVDIHKNLGF
                     RVSLDNLASASLGATKTADGIQSVQWFRNGELDKVAEYCKADVDITRRVFEFGRDNGF
                     VYYKSKLGSKLKTPVKWK"
     gene            complement(5197..>5810)
                     /locus_tag="PLT08_03820"
     CDS             complement(5197..>5810)
                     /locus_tag="PLT08_03820"
                     /inference="COORDINATES: protein motif:HMM:NF014069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HNE03619.1"
                     /translation="RSLLMFSALYHDVCKPDTKTIDENGRIRFFDHDIKGAEVTADRA
                     RAFNLSNDEVERLHTIVRHHMRFHFFADRLEREGQPPSRKAVYRFFRDSGAAGIDLIL
                     LALADLRGTKGNELTQVTWTAYLDIARILLENYWEHPEEVVNPPRLIDGNELMKELNL
                     QPGKVVGTLLESIRENQAAGKIVDKEQALAFAREELAKGVNEA"
BASE COUNT         1478 a         1478 c         1307 g         1547 t
ORIGIN      
        1 tcttttgatg taacatctgg atagggcttg gctaccggtg cagagattgg aatgttttga
       61 tgttgaattt cagagggggt aacaggttgt agctcagtgg ctggcgcttc ggtagacgga
      121 agtgcagtct caattggttg aaccgcactt ggtaaattgc atccttgtaa tattaggaga
      181 acaagaatgc cagcgaagaa tcgaacagtt ttgcttttca tatattattc tccttgttac
      241 ttaattatta caatacaaga gattaaattc aaggttacaa aagaactatt gagaactaca
      301 acaaaaatcc ccgcctttcg gcagggattt ttgttgtagt tttatttttt gcttttctta
      361 gcaactttct tcgctgcttt cttcacaact ttctttacaa ccttcttagc agcgggtttc
      421 ttcgcaacct tcgccttttt cgcaggctgg tgcttcaacg cggcatgagc tgcagcgaga
      481 cgtgcaactg gcacacggaa gggggaacag gaaacatagt tgttaccaat gaggtgacac
      541 cattcaatag agtcggggtc gccgccatgt tcaccacaga tacccacttc aagatcaggt
      601 cgaacggcac gtccttcaaa gatggcgcgt tgcattagct tgccaacacc gttgcgatcg
      661 agagtctgga agggattctt gacgaggata ccttcctgca cataagtaat gaggaagttg
      721 cgttcggcat cgtcacgcga ataaccgaag gtcatttgtg taaggtcatt cgtaccaaag
      781 gagaagaact gcgcctgggt ggcaatgtgg tctgctgtaa cagcagcacg cggaatctcg
      841 atcatggagc caaatttata ggcgaacttg atcttctttt cctgcatcac ggctttaccg
      901 atgcgttcga ggcgcggctg gatccagtct aactctttca cggttccggt caatgggatc
      961 atgatctctg gcttgaccct gataccgcgc agggtgcagt cggcggcggc ttcaaagatg
     1021 gcacgcactt gcatttccac gatctcgggc atcgagatgc tcaaacgcac gccgcgcaaa
     1081 cccatcatcg ggttggattc gtgcatgccc ttgatcgatt caagcagttt ttctttttct
     1141 gccaagtctt tcgtctcacc cttcactcgc atggtaataa cttcttcaag gagtttttct
     1201 tcgtcgggca tgaattcatg caaaggtggg tcgatcaaac gaataatcac ggggtaaccg
     1261 tccatcgctt cgaagagacc gtcaaagtcc ttacgctgat gcggtaagag caagttgagc
     1321 gcttcagtac gtccttcgct ggtttcagcg agaatcatgc gttgcacaat cggcaagcgt
     1381 tcaggttcaa agaacatatg ctcggtgcgg cacaaaccaa tacccacagc gccataagag
     1441 cgagcacggc gagcatcttt tggataatca gcatttgccc aaacttgtaa accacgggtt
     1501 gggtaaccgg gctccacttc ttcgcgaata tttttgcgag cggaaatgtc atctgcccac
     1561 ttgagcaggg tcattaattc tttctgttct tcaagagaag gttcagaggt tgggatcttt
     1621 ccagcaaaga ctttaccagt cgtgccgtct acagagaccc aatcaccttc gttaagcaca
     1681 atttcaccca catgcataat gcgcttttca aggtcgatct tgatggcagt agcacccacc
     1741 acgcatggga taccaaattg gcgagccaca acggcggcgt gagaagtagc gccaccttca
     1801 ctggtcaaca cacccttggc agcgatcatg ccgtgaacat catctggctt ggtaaatgga
     1861 cgcaccatga tggtgtcttg cttgtgttcc ttggcatatt cttcagcttt atcggcatca
     1921 aaataaattt gaccaacagc cgcacctgga gaagcattca cacccttggc aaggaactta
     1981 ccttcttttt cagcctgatt catagcttca tcgttaaatt gcgggtgaag taaggcatcc
     2041 acctgatctg gggtgacacg ttgaaccgcc tgatctttgt tgatcaagcc ttcatttgcc
     2101 atatccacag cgatcttcac agcggctttg gcagtgcgtt tacccacacg agtctggagc
     2161 atccacaact tgccgcgttc aatggtgaat tccacatcct gcatatcggt gtagtgcttt
     2221 tcaaggcggg cggtaatttc catgaattga gcatatgcct tgggcatata cttcttgagg
     2281 ttttcaatgg catcggtatt gcgaatacca gccaccacat cttcaccctg cgcattgagc
     2341 agatactcac ccatcatctt cttttcgcca gtggatgggt tacgcgtgaa tgccacaccg
     2401 gtagcagagt catcgcccat gttcccgaag accatcgtac aaatattgac ggcggttccg
     2461 agcgcatcag agataccttc cttgcggcgg tatgcaacag cacgttttcc attccaggag
     2521 cggaatacag cttcagttgc taatgagagt tgtttataag gatcttgggg aaattcctga
     2581 ccacaggctt tcttgtacgc ttgcttataa atccccacaa cttctttcca atcttccgcc
     2641 ctcatttcaa tatcgagctt atagcctttt ttctctttat atgcttccaa tggatgttcg
     2701 aaggcttcgt cttcgatgtc catcacgacg gttccgaaca tttcaaccac acgtcgatat
     2761 gagtcccaca caaaccggtc gttattcgtt gccttgatca taccttctgc tacgatatcg
     2821 gtcagaccaa tattgagaat ggtattcatc atgcccggca ttgagaactt agcacctgag
     2881 cgacacgaga ccaaaagcgg atttttcgga tcaccgaatt ttttgccggt cttgcgctcg
     2941 atctctttca gcgctttcaa ttcctgctcc cacattcctt gcgggaattt cccagctttt
     3001 tgatatgcat tgcacgcttc tgtagtaacg gtaaagaaag gtggaaccgg cacattcgcg
     3061 cgagtcatat ccattaagcc cgcacctttg ccgccgacga tggctttgac accatcccat
     3121 gagcctttgg ctaatttttc aacttccttc acctcattat agagataaac ccatttagac
     3181 atttcgacct cctgatcgag taatattgtc actctatatc ttgattgtga aatattgtac
     3241 caaaaacagt ccaattagca cctgacaaaa atcatgctaa gagtggatgt caatcaggga
     3301 aattactaat tcttattcaa aagaacctgt taatacattg caaagtacat tgtcagtcaa
     3361 atcagggata atgtgcctag atattatgac acctacagca aaaatccttg tgatcgatga
     3421 tgatgctgcc atcacagagt tgatgagcat gttactaaaa acctttgggt ttgaagtgct
     3481 cacaaccaac attggcgaag aaggcatccg ccttgcgcgt gaagccaacc caaacgtcat
     3541 tctactcgac ctaatgatgc ctgatttaga cggatggcag gtttgcaaag aaattcgcaa
     3601 aacaagtacc gtgccgatcc tgattctttc tgcaatcaac gatccgcgca tggtcgctag
     3661 cgttttggat gttggcgcag acgatttcct cgtcaaaccg gtgccaagcg gcgtgttggt
     3721 tgcgaatatc aagaaattga ttcgacgcaa cacaggcgca ctggatatgt ccaagttaaa
     3781 aaaaacgacg gcttccctcc aacaagctaa tcacgcgctt cacccctagc ccgaagctgg
     3841 ttctacaaaa taaaaatgcc catccccgtt agctggatgg gcattttctt tgctatttga
     3901 gctttttcaa cgccgaagcc gtttcgtttg gcatttccag catgggcata tgccccaccc
     3961 cagccaactc caccaactgc gcagacgaga ccagtgactt aatctcccgc gcacgttcga
     4021 gcggtatcaa ctcatctgca tctccgtgaa tcaacacaag aggctgagca taagatgcta
     4081 tgaacgaagt catatcgtca cgctctgcca tggctttgag tgcccccgtc acaccagcca
     4141 cgctttgctt cgcaatcaac gggcggacaa aatctcgcac agactgattg ggcgaaagtt
     4201 tatccgtcat cgcatcggcg acgatctgaa ctcctttttg aacaacatct gccgccgttt
     4261 tatatctacc ttctttacgt tcaggggtat ctgaggctgc ttgagaggaa accaaagcca
     4321 aaccggtcac acgctgtgga aaagacttcg caaatgcgag agcgatataa cctcccatgg
     4381 aatgaccagc caaggctgtt ggacccactc cgagcgagtc cagaagcgcg gcaaggtcgt
     4441 tggcaatgtc tgagagggag taaggtgtgt caacagtggt tgatttgccg aatccgcgca
     4501 catcggggat gatcaggtcg aaatcatttt cgagttgagc agcgacatca ttccaaatcg
     4561 aatggtccaa aggatagccg tggatgagca tcaaaggggt tcccatgccg cgccgttcgt
     4621 atgccagttc aataccgtta agtttgattt tgttcatatc gctttacttc cacttgaccg
     4681 gggttttcag cttgctgccc agcttagact tgtaatatac gaagccatta tcgcgcccga
     4741 attcaaagac gcgccgagtg atatctacat cggctttgca gtattcagca actttatcca
     4801 gctcgccgtt acgaaaccac tgcacagact gaatgccatc tgctgttttg gttgccccaa
     4861 gagatgcact cgccaaatta tcaaggctca ctctaaaccc taaattcttg tggatatcta
     4921 ccaacatatc caaagttgga atggatgcga gccgaacctg atttgcatac ggctgcaaga
     4981 ctcgataatc aaagctaatg aggttaaacc cgatgacctt ggtagcagct tttaactctg
     5041 ccagtaaagc agggacatcg ttctcccaat acactgtaaa gtcatttttt gcagtggaat
     5101 atgtgaccgc gcatgcgacc ttcaatttat cggcatgatc acgcccgcct acttcatcaa
     5161 aaagaaattg agactcgagg tcgaagaata catagttcat gcttcattca ctcctttcgc
     5221 aagttcttca cgcgcaaacg ctaaagcttg ttctttatcc acgatcttgc cagcggcttg
     5281 attctcgcga atcgactcaa gcaacgtccc aacaaccttt cctggctgga gatttagttc
     5341 tttcatcaac tcgttcccat ctattaaccg cggcgggttg acaacttctt cggggtgttc
     5401 ccaataattc tccaacaaaa tacgcgcaat atcgagatac gccgtccaag tcacttgggt
     5461 cagttcgttg cctttggtac cgcgcaaatc agccaaagcc agaaggatca aatcgatgcc
     5521 agcggcgccg ctatcgcgaa agaagcgata taccgctttg cgagaaggcg gctgtccttc
     5581 tctttccaag cgatcggcaa agaaatggaa acgcatatga tgccgaacga tagtatgcaa
     5641 ccgctcgact tcatcattgc ttaagttgaa agctcgcgcc cggtctgcgg tcacctctgc
     5701 ccctttgatg tcgtggtcaa agaagcggat gcgcccattt tcgtcgatgg ttttggtgtc
     5761 tggcttgcag acgtcatgat ataaagcaga aaacatcaac aacgaacgat
//
LOCUS       DAOQJE010000096         5806 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_7901_length_5806_cov_4.342549, whole genome shotgun sequence.
ACCESSION   DAOQJE010000096 DAOQJE010000000
VERSION     DAOQJE010000096.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 5806)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5806)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5806
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7901_length_5806_cov_4.342549"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..968)
                     /locus_tag="PLT08_03825"
     CDS             complement(<1..968)
                     /locus_tag="PLT08_03825"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021134.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 87 protein"
                     /protein_id="HNE03620.1"
                     /translation="MIKINIQKILRLLSDTRPQVWLFAGFLIVYVLYFIIPIFFSSQV
                     MQFPTYVPVMRTIGGDLSQILDYSKSWAILRDTPYIGENPYPPFVAVFFTPFLFIPFS
                     LAYKFIVFLSLLFFVLGIVFLTVQFKNDSNFPFIFSFVFFTGLFSYGLHFELERGQFN
                     LIAMFFCYMAVWVYHSHNKFRYLAYILFALSVQLKLYPLIFIVMFVSDWKDWGRNVKR
                     LLLLCVGNFALFFILGTKIFSDFVAALRKFMLAGSVGGSHSIRAFVHMLVDLATENNW
                     IWMRDYSPSIQLILLIIVLTCIFVVLVQTFKQNSGKINPQLLLACAL"
     gene            complement(1023..2933)
                     /locus_tag="PLT08_03830"
     CDS             complement(1023..2933)
                     /locus_tag="PLT08_03830"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HNE03621.1"
                     /translation="MMNSFSTFRDLLMKVELSLRHNLFLPAFGLLPLAYVFYAGFIDL
                     PPLLNTPFVFFLLFAGLALVLHWRQGYFIPLLLVGYLGLFFLPLTWQWRGLEFDTNLL
                     MGVFPFNDGMYYLTDAYRLNAGLGTMILQNGRPIYPGLLAFFMWIFGGNLQAALAVIC
                     IVTAFSIFVLALEIREISGPYSAALLTAILFYFAYAYVGRVHTESLGLAFGTLGLTLL
                     LRGARKNNIWALVFGALALSMGLNVRAGAFFTLPFLLCWGWIKRETFGRKLLFLLILS
                     ILAGFLVNTFLMDKFAVVGFFEERTPFSNFGFSLYGLAAGYKTWGYIFAVHPELTNYS
                     NAWPYAWDLVRQQPLMLLWGIFLSYKDYFSPNGMFYIMRLGDQQIWVSWILYVLMFIG
                     FYRLLKLRYEEHWFMFLFLLFGVLLSMSMIPYNDNGLRILMTTNPINILVVSLAFAIP
                     SKPLTYEEYENTNPEFIAGLVLILTLMCTVGPSVVTNLHKEIPPLDKLNCPEGTEQIS
                     LYVMSGSYINVVKVGPEFGFLPEVKKIDIKARLDDYYIRGEVPYDPLTNFPAFERMLR
                     KLKQGDSIIMGFNLVDLNKSYGPEKFVFVITRTKQIQKIGDVNNFCARLPSDERFRSN
                     LFYFDQSVDILE"
     gene            complement(2966..3574)
                     /locus_tag="PLT08_03835"
     CDS             complement(2966..3574)
                     /locus_tag="PLT08_03835"
                     /inference="COORDINATES: protein motif:HMM:NF019846.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HNE03622.1"
                     /translation="MNKFSSEMRGVVIDFGGKRENKRGTFQPPETQALVWWYLNLDLS
                     TYPNIFADVNYTPLQTSGVDCILCTEVLEHIPNPQSCVDEIRRTLKNDGVVFVSVPFF
                     YPIHADPNDFQRYTEDGLRYLFRDFKSIDVFRMGGYLGSLGLMLELGIAGVLGNGIKE
                     KILRWIMKWSSRFLITWDIICLDRETEQHTKFTTGYFLRAVR"
     gene            complement(3661..5439)
                     /locus_tag="PLT08_03840"
     CDS             complement(3661..5439)
                     /locus_tag="PLT08_03840"
                     /inference="COORDINATES: protein motif:HMM:NF014589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyltransferase C-terminal
                     domain-containing protein"
                     /protein_id="HNE03623.1"
                     /translation="MQNIKSVLGVTLGHDTSFAHVVNGKIVAIMEAERYFRQKRYKLH
                     SQTMEPGKHISGYQYVSLEDMELFLGLVAKEWGNTFDALAVQNQGRIEEFKNFQQILS
                     RLGFKFSSVHHVDHHLSHAALSFFSSPFKEALVLSYDGEGNDGQTIIFGAKGNKLEYL
                     HNDPIRFGQSYNNMGYIVGIKPEVEGTTSGKVMGLTAYGEPVEDWMPYAREYIKKYIK
                     ATPRQIEGLNKYGKAHRINDIAVRDIPDLKQYLVPEEDSDGGLKSAVKKMIGRTNTIL
                     RLNGPEDKTAQNFAATVQVAWTKAVIDLLKPYFGKFENLCVVGGCALNGVTNYEIQQL
                     GVFKATHFVPNPSDCGLSAGAALYVYWNFGNNGTFEGYGDYFSPYLGSEVFDKSDLPA
                     LRKQYPHLELDADRTASILAGLVHTDNMVGVIRGRYEVGPRALGNRSILCNPLNKDMK
                     DIVNKKVKHREWYRPFAPIAIAERAADYFTNTTDIPYMSVICYTKPEYQAVLPSITHV
                     DGSSRLQTVSSEAHPFMHKTLKEFERISGMPIMLNTSFNPGGEPILNYYSVGLDMLKT
                     TELDFVLIENTLFCKIEREEILRSIN"
     gene            complement(5557..>5806)
                     /locus_tag="PLT08_03845"
     CDS             complement(5557..>5806)
                     /locus_tag="PLT08_03845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE03624.1"
                     /translation="NDNIGSKAKVRGNKRYGVYTPPLQGDFYYKIYEQKDVANLQKRN
                     LPVSFEEFVVPIGFGLPLDSEGNLLKSSFNDTIVADVN"
BASE COUNT         2051 a         1196 c          973 g         1586 t
ORIGIN      
        1 agcgcacaag caagaagcag ttgggggttg atttttccgc tattttgctt aaacgtttgt
       61 actaatacta caaatatgca agtgagcact ataattagta gaatcaattg tatagatggg
      121 ctataatcgc gcatccatat ccaattgttt tctgttgcga gatccacaag catatgcaca
      181 aaagctctaa tagagtgact ccctccaaca cttcctgcca acataaactt tcttaatgca
      241 gctacaaaat cactgaatat tttcgtcccc aatataaaaa acagggcgaa attgccaaca
      301 cataatagaa gcaatctctt aacatttctt ccccaatctt tccagtctga gacaaacatt
      361 actataaata ttaatggata aagcttaagt tgtactgata gtgcgaatag tatgtaagcc
      421 aagtaacgaa atttattatg ggaatgatat acccacaccg ccatatagca aaaaaacatg
      481 gcaatcaagt tgaattgacc tctctctaat tcaaaatgaa gtccatatga gaataatcca
      541 gtaaaaaaca caaaagagaa aatgaagggg aagttactat catttttaaa ctgaactgta
      601 agaaatacta taccgagaac aaaaaacaac agacttaaaa aaacaataaa cttataagcc
      661 aaagaaaagg gaataaatag aaacggagta aaaaacacag ccacaaatgg cggatatggg
      721 ttttcaccga tatagggagt atctcttaaa atagcccatg atttgctata atctaatatt
      781 tgagataaat caccgccaat agtacgcatc acaggaacat aggttggaaa ttgcattact
      841 tgagaagaaa agaaaattgg aataataaaa tacaacacat acacaatcaa aaaaccagca
      901 aaaagccaaa cttgagggcg tgtatctgag agtaatctta atattttctg aatatttatc
      961 ttaatcatta tttttcctaa cttattaaat atattacttt tcgacaaata atcaaaagta
     1021 acttattcta gtatgtctac agattggtcg aaataaaaaa gattactccg aaaacgttca
     1081 tcagagggca acctagcaca aaagttatta acatcaccaa ttttttgaat ttgttttgtt
     1141 cgagttataa caaaaacgaa cttttcagga ccataacttt tatttaaatc tactaaatta
     1201 aaacccataa tgattgaatc accttgttta agttttctca acattctttc aaaagcaggg
     1261 aaattggtca ggggatcata aggaacttca ccacgtatat agtaatcgtc taaccgtgcc
     1321 tttatatcga ttttcttaac ttcaggcaga aacccaaatt caggtcccac ttttactaca
     1381 ttaatataac ttccagacat cacgtataaa gaaatttgtt cagtaccttc aggacaattt
     1441 aatttatcta atggtggaat ctctttatgt aaatttgtta caaccgaagg tcctacagtg
     1501 cacatcaggg ttaatatcaa aaccaaaccc gctatgaatt ccggattagt attttcatat
     1561 tcctcatatg taagaggttt agaaggtatt gcaaaagcta agcttaccac cagaatattg
     1621 attggattgg ttgtcatcaa tatacgcaat ccattatcat tgtaaggtat catcgacatg
     1681 gaaagcaaaa caccaaacaa taaaaagaga aacataaacc aatgttcttc atagcgaagt
     1741 ttcagtaacc gataaaagcc aataaacatc agaacataca aaatccaaga aacccatatt
     1801 tgctgatcgc caagccgcat aatatagaac attccgtttg gcgaaaaata atctttataa
     1861 ctaaggaaaa tgccccaaag caacataaga ggctgttggc gaacaagatc ccatgcgtaa
     1921 ggccatgcat tggaataatt agttagttca gggtgcacag caaaaatata tccccatgtt
     1981 ttatatcctg cagccaatcc ataaaggcta aatccgaaat tggaaaatgg tgtcctctct
     2041 tcaaaaaaac caactacagc aaatttatcc attaaaaagg tattaaccaa aaagccagct
     2101 aagattgata gtattaataa aaatagcagt ttgcgcccaa aggtttcccg ttttatccat
     2161 ccccaacaaa gtagaaaagg caatgtaaag aatgcaccag ctcgcacatt gagtcccata
     2221 gataacgcta gtgctccaaa tacaagtgcc caaatattat ttttcctagc accccttaat
     2281 aataacgtca aacccagtgt tccaaaagcc aaaccaaggc tttcagtatg cacacgccca
     2341 acataggcgt aggcgaagta gaataaaata gcagttaaca acgcagcaga atagggacca
     2401 gaaatttctc gaatctctaa agctaataca aaaattgaaa aagctgtgac tatacaaatt
     2461 actgccaagg cagcttgcaa attaccgcca aatatccaca taaaaaatgc aaggagtcct
     2521 gggtagatgg ggcgcccatt ttggagaatc attgtaccca aacctgcatt tagacgatag
     2581 gcatcagtaa gatagtacat cccatcgtta aagggaaata cccccattaa gagattcgta
     2641 tcaaattcta gccctcgcca ctgccatgta agaggcaaaa aaaataaacc aagatatccc
     2701 actaaaagta gaggaataaa atacccttgt ctccaatgaa gcaccaacgc caacccagca
     2761 aaaagtaaaa aaaatacaaa aggggtgttc aaaagaggcg gcagatcgat aaaaccagca
     2821 taaaaaacat atgcaagcgg gagtaaacca aaagctggca aaaacaggtt gtgccgaaga
     2881 cttaactcaa ctttcatcaa caaatcccta aaagtagaaa agctgttcat cataatcatt
     2941 aatatcccat aaatcctggt attgttcagc gaacagcacg caaaaaataa ccagtggtaa
     3001 attttgtgtg ctgttcggtt tctctatcta aacaaataat atcccacgta attaaaaaac
     3061 gtgaagacca tttcataatc caacgcaata tcttttcttt aattccattt ccaagcacac
     3121 cagcaattcc aagttccaac attagaccta atgagccaag atatcccccc attctaaata
     3181 catcaataga cttaaaatca cggaagaggt aacggagtcc atcctcagta tatcgttgaa
     3241 aatcgttagg atcagcatga atgggataaa agaaaggcac tgaaacaaag acaactccat
     3301 cattcttaag tgtacggcga atctcatcta cacaagattg aggatttgga atatgttcca
     3361 atacttcagt acaaaggata caatcaaccc ctgatgtttg taagggcgtg taatttacat
     3421 cagcaaatat gtttgggtag gttgaaaggt caagattgag ataccaccat actaatgctt
     3481 gtgtttcagg cggctgaaaa gttccacgtt tgttttcgcg ttttccgcca aagtcaataa
     3541 caacgcctcg catctcgctg gaaaatttat tcaagtagtg atcaagccag aaacgccggt
     3601 aagttatgta acccttagac ttccaagggg atgaaaatga tgagatgcga gacatatgta
     3661 ttaattaatt gaacgcaata tttcctcgcg ttcaatttta caaaacagtg tgttttcaat
     3721 aaggacaaaa tcaagctctg tagttttaag catatctaat ccgacagaat aataattcaa
     3781 aatcggctcc ccccctggat taaatgaagt attgagcatg ataggcatac cagaaatcct
     3841 ctcaaattcc ttcaatgttt tatgcataaa gggatgtgct tcactgctaa ccgtctggag
     3901 gcgggagctt ccatccacat gagtgatgga aggtaaaaca gcttggtatt caggcttggt
     3961 ataacaaatc acagacatgt aaggaatgtc cgttgtattg gtgaaataat cagctgcacg
     4021 ttctgcaatg gcgattggtg caaaggggcg ataccattca cgatgcttaa cttttttatt
     4081 cactatatct ttcatatcct tattaagtgg gttacacaaa atcgaacggt ttcccaaagc
     4141 tcgtggaccc acttcatatc tcccacgaat aacacccacc atgttatctg tgtgaactag
     4201 tcctgctaga atgctagcag tacggtctgc atcaagttca agatgggggt attgctttcg
     4261 caaggcaggg aggtcactct tgtcaaaaac ttcagagcca agataaggag aaaagtaatc
     4321 tccataacct tcgaaagttc cattatttcc aaagttccaa tagacataca gagccgcacc
     4381 agcagagaga ccacagtcag atgggttagg cacaaaatga gtcgccttga aaacacctaa
     4441 ttgttggatc tcataattcg taacaccatt taatgcacat ccgccaacaa cacataaatt
     4501 ctcaaattta ccaaaatagg gcttaagcag gtcaattaca gctttagtcc atgcaacttg
     4561 aacagtagca gcaaagttct gagccgtttt gtcttcagga ccattcaacc gcagaatggt
     4621 atttgttctc cctatcattt tcttaactgc agatttcaac ccaccatcgc tatcttcttc
     4681 aggaacaaga tattgcttaa gatcgggaat atcacgaacc gcaatatcat taatacggtg
     4741 ggctttacca tatttgttga gaccttcaat ttggcgaggg gttgccttta tatatttttt
     4801 aatgtattca cgcgcatagg gcatccaatc ttccacaggt tcaccatatg ccgtaagtcc
     4861 catcacctta ccagatgtag tgccttcgac ttcaggcttg ataccaacaa tgtatcccat
     4921 attattgtaa ctctgtccaa aacggattgg atcattgtgt aagtactcaa gcttattacc
     4981 tttggcgcca aagatgatgg tctgaccatc attaccttca ccgtcatagc taagaacaag
     5041 cgcttcctta aaaggtgatg aaaagaaaga taacgcagca tggctaagat gatgatcaac
     5101 atgatgaact gaactgaact taaatccaag acgagataaa atctgttgaa aattcttgaa
     5161 ttcttctatg cgcccttgat tttgaacagc taacgcatca aaagtatttc cccattcctt
     5221 agctactaaa ccgaggaata actccatgtc ttccagagaa acatattgat acccactaat
     5281 atgcttacct ggctccatcg tctgactatg taacttataa cgtttttgtc taaaataacg
     5341 ttccgcttcc atgatagcca caattttccc attaacaaca tgagcgaaac tagtatcatg
     5401 ccccaaggtc actcctaaga cacttttgat attttgcata ttttttcctt ttcaaaactg
     5461 aaggtagaag gtttttatgt ttatttttca gaaacaccaa ggcaagaatt atactatttc
     5521 agtaattatg gtgcataaaa ttgcagataa tgcaaattaa ttaacatcgg caactattgt
     5581 gtcattaaag ctacttttca gcaaatttcc ctctgagtct aatggtaagc caaatccaat
     5641 tggcacaaca aattcctcaa aagacacggg cagatttctc ttttgcaaat tagcaacatc
     5701 cttttgctca taaattttgt aataaaaatc cccttgtaaa ggcggagtat agacaccata
     5761 tctcttattt ccgcgaactt ttgccttgga gcctatattg tcatta
//
LOCUS       DAOQJE010000097         5792 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_7924_length_5792_cov_6.687816, whole genome shotgun sequence.
ACCESSION   DAOQJE010000097 DAOQJE010000000
VERSION     DAOQJE010000097.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 5792)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5792)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5792
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7924_length_5792_cov_6.687816"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1377)
                     /locus_tag="PLT08_03850"
     CDS             complement(<1..1377)
                     /locus_tag="PLT08_03850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE03625.1"
                     /translation="MNEFDKNKKLETLLEETAEQLQPNVMFKAELEEKLRKAHKPRAS
                     LPTFAFRNWLPALTGIVVVGGLVIFMLWLFQTLEPQKSVGTNEEQPIEVQTTPGVIID
                     PNNTNSNETIGGYDYRGGKLFLTSALPESSATANIYEYIADQPATIEEARALANQFGL
                     QGEVYTTAFPQAPDKIGYVISDGKRMLTVYTKNYFTYTPDILVTDRGYYGVNNDNAET
                     VISDFFNQNGLTFPFSISKSPAKGMYHFMQLSPDGIPMQYDIYTIQSSNVTLGQNGEV
                     AYLSANMLSYNPQAFGSYGIISAEDALNAILDPHSTVGMTESGSGGEGGVIPQQWYRE
                     YPNNETVSISGSLIKYDSATAGQPPLIFVGSFEALGNTTDLQALSNFAFVQVTGQFVE
                     ENGIRKFNVESTNANVIQGYITGSLRKEGDQIILTSDDGNSEYVLLDPPADLPLNTVP
                     TESYLGISG"
     gene            complement(1374..1946)
                     /locus_tag="PLT08_03855"
     CDS             complement(1374..1946)
                     /locus_tag="PLT08_03855"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HNE03626.1"
                     /translation="MTTLMNMEIKELDEEDLARKAISDADAFAELYRQHVTRVYRYHM
                     AHIGNVKDAEDLTSQTFMAAMESIRSFRGDGSFAAWVMGIASKKRLMYFRGNRHEVPL
                     EAAEHYPNPSLPTDKAAMQRIRLQSISHALKQISSERSEAIVLIYFGGLSYAEAAGVM
                     RKNEASVKMLVSRGLQDLRERTSLRMEVEE"
     gene            complement(2051..2527)
                     /locus_tag="PLT08_03860"
     CDS             complement(2051..2527)
                     /locus_tag="PLT08_03860"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF015048.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HNE03627.1"
                     /translation="MSKIKQPNSRHCFICGVENPVGLHLHFYETEPGVIETEYTAPDH
                     FEGFPGVLHGGIVAAIIDEVSGRAYMGNDPANPRFMFTGKLEIKYRKNVPIGKLLRIV
                     GKVMENKGRIAKAWGGIYDAETNELLAEGTGMHINVPSDQFDMSKLEELGWKIYPD"
     gene            complement(2527..3219)
                     /locus_tag="PLT08_03865"
     CDS             complement(2527..3219)
                     /locus_tag="PLT08_03865"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone/menaquinone biosynthesis
                     methyltransferase"
                     /protein_id="HNE03628.1"
                     /translation="MTQLTGQERAQYVQSMFSKIAHRYDLMNRLMTGGQDIRWRREVI
                     SLTKIRPHGSILDLGTGTGDLAREALTQYPKAQVTAADFTLEMMRVGKKNGDLNFSTA
                     DALNLPFKGKNFDAVVSGFLMRNVTDVQQALREQHRVLKPGGRIVILDTTRPKKNLLS
                     PFIKFHMHVIIPMVGGMLSGMRDAYEYLPDTTENFLTAEELSVRMVAVGFRRVNFKRL
                     MLGTIAIHWGEK"
     gene            3280..4542
                     /locus_tag="PLT08_03870"
     CDS             3280..4542
                     /locus_tag="PLT08_03870"
                     /inference="COORDINATES: protein motif:HMM:NF014862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HNE03629.1"
                     /translation="MSTTSYSFARESQLALDQARRIAEGNKLPFVDSLMLLLTLLQLK
                     NSRVQALLKSLNVRVDNLAARIVATIKLEAKEILQVSEGKPVLSSECETVLALASAEM
                     QLAGQNSISEFSLLLGMLQNTESKAGQILSQYAVAVDQAREKIKIFKETPVSQSNILR
                     KDGFLRRAFRNGISPIFISLVLFTIAMAAFLWLGIGNNPRAFLIAFIIGGWIISVALH
                     EFGHAITAFWGGDESVEDKGYLTLNPLKYSNPVLSVIIPVMMLLLGGIGFPGGAVYIN
                     IFALRKPKYRSLVSAAGPLANLLVAILLAIPFANFPFFFFFIYAPQKFLMALAFLAFL
                     QITSIIINLLPIPGLDGFGILEPFLPREIVGIASFIRPFGFLILYLFVFIDSPISNMF
                     WQSIWAITNLLSPSLAYYANEGLKLFLN"
     gene            4550..5449
                     /locus_tag="PLT08_03875"
     CDS             4550..5449
                     /locus_tag="PLT08_03875"
                     /EC_number="2.5.1.74"
                     /inference="COORDINATES: protein motif:HMM:NF004751.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0046428 - 1,4-dihydroxy-2-naphthoate
                     octaprenyltransferase activity [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoate
                     polyprenyltransferase"
                     /protein_id="HNE03630.1"
                     /translation="MTETNKPNMFQIWWLAIRPRTLPAAAAGVAMGSALAWHDGSFQL
                     LPALAALLVALLLQVGSNVANDVFDFERGADTAERLGPVRVTQAGYLTPSQVKTGMVV
                     IFAFAAVLGLYLATLRGWTVIWLGAAAILSAVAYTGGPYPLGYHGLGDVFVFLFFGLA
                     AVAGTYFVQVGSVSPAAWWMAVPVGLIVTAILVVNNLRDLENDRKAGKRTLAVRFGEE
                     GAKAEYLLLMLIAYLIVPVLVWMNIIPIGALLAWLSLPIAIRTLRTVFTQKGKPLNVA
                     LAGTGQTAFLFSLFFWVGLLFSN"
     gene            complement(5442..>5792)
                     /locus_tag="PLT08_03880"
     CDS             complement(5442..>5792)
                     /locus_tag="PLT08_03880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HNE03631.1"
                     /translation="LVRDQASGIAPQLLQSLQSLGAGRRALFRFVYLPASLPAILTAL
                     RQSIGTAVAVLYITELSATKYGLGYYIYYKGSTLLDYPSMYAGVIAMSLLGLGMYFTV
                     DWLERKWCPWKFVS"
BASE COUNT         1350 a         1347 c         1397 g         1698 t
ORIGIN      
        1 tccagagata ccgagatatg actccgtagg aaccgtatta aggggcaagt ctgcgggtgg
       61 gtctagcaaa acatactcgc tgttgccatc gtcactggta aggatgatct gatcgccttc
      121 cttgcgcagc gagccggtta tatatccctg aataacattg gcgttcgtgc tttccacatt
      181 aaatttgcgg atgccatttt cctcaacaaa ttgacccgtc acttgtacaa aggcaaagtt
      241 gctcagagct tgtaagtctg tggtgtttcc aagtgcctca aaactgccaa caaaaatcaa
      301 cggtggctga cctgctgtcg cagagtcgta ttttatgagg ctgccagaga ttgaaacggt
      361 ctcattattt ggatattctc gataccattg ttgtggaatc acgccgcctt ctccgccaga
      421 gccgctttcg gtcatgccga cggtgctatg tgggtcgagg atggcattca aggcatcttc
      481 cgcgctgatg atgccatagc ttccgaatgc ttgtgggttg tagctgagca tatttgcaga
      541 gagataagct acttcaccat tttgtccaag cgtgacattt gaactttgga tggtgtagat
      601 gtcatattgc atggggatgc catctggcga gagctgcatg aaatgataca taccctttgc
      661 aggagacttg ctgatgctga atgggaacgt taacccgttc tgattgaaga aatcagaaat
      721 gaccgtctct gcattgtcgt tattcacacc atagtaaccg cggtctgtta ccaaaatatc
      781 gggtgtgtat gtgaagtaat ttttggtata cacagtaagc attcgcttgc catctgaaat
      841 gacataacca atcttatcgg gcgcttgtgg aaatgcggtt gtatatactt caccttgtaa
      901 cccaaattga tttgcaagcg ctcgcgcctc ttcaatggta gcgggctgat cagcaatgta
      961 ctcatagata ttggcagttg ctgaactctc aggcaaagca gatgtaagga acaacttccc
     1021 gccgcgatag tcatatccgc caatggtttc gttgctattt gtattattcg ggtcaatgat
     1081 gacgcccgga gttgtttgca cttcaatagg ttgttcttcg ttagtgccaa cgcttttttg
     1141 tggctccaac gtttggaaga gccaaagcat gaaaattacc aatccgccaa caacgacaat
     1201 acctgtcaac gcgggcaacc agtttcgaaa tgcaaaggtt ggcaaacttg cgcgtggctt
     1261 atgcgcctta cgaagcttct cttccaattc ggctttaaac attacattgg gttgtagctg
     1321 ctcggcagtt tcttccaata acgtttcgag ttttttgttt ttatcaaatt cgttcattct
     1381 tccacctcca ttctgaggga ggttcgctcg cgcaggtctt gcagcccgcg tgagacgagc
     1441 attttgacag acgcctcatt ttttctcatc acgcctgcgg cttcggcgta gcttaatccg
     1501 ccaaagtaga taagcacgat cgcttcagag cgttcacttg aaatttgctt gagcgcatga
     1561 gaaatagact ggagccggat gcgttgcatt gcggctttat cggtaggtaa gctcgggttg
     1621 ggataatgct ccgccgcctc taacgggacc tcgtgcctgt tcccgcgaaa atacataagt
     1681 ctcttctttg aagcgattcc catcacccac gccgcaaacg agccatctcc tcgaaacgag
     1741 cggatacttt ccatggctgc catgaaggtc tgcgaggtta agtcttcggc atcttttacg
     1801 ttgccgatat gtgccatgtg atagcggtat acccgcgtca catgctgacg gtaaagctct
     1861 gcaaaagcat ctgcatctga gatggctttc cgtgctaggt cttcttcgtc tagttctttg
     1921 atttccatat tcattagggt ggtcatatac ctcctgtgag tagtgcgcac atagatatgt
     1981 tgcaggaggt tgaaaaaagt aacaggcaaa ataaaaggtg acagtcactt tataagcgac
     2041 tgtcacctgg ctagtcaggg tatatcttcc aacctaactc ttcaagcttg ctcatatcga
     2101 actggtcact tggcacattg atgtgcatgc ctgtcccttc ggcaagcagt tcattcgtct
     2161 ctgcatcgta aattccgccc caggctttgg caattcgacc cttattctcc attactttac
     2221 caacgatcct gagcaatttg ccaatcggca cattcttgcg gtatttgatc tctaacttgc
     2281 ccgtaaacat aaaacgcgga ttcgccggat cattgcccat gtatgcccgc ccagagactt
     2341 cgtctatgat cgcggcgacg atgcctccat gcaacacacc agggaagcct tcaaaatggt
     2401 cgggcgcggt gtattcggtt tcgatgactc ccggttcggt ttcataaaaa tggaggtgta
     2461 agccaacagg gttttcaact ccacaaataa agcaatgacg tgaatttggt tgtttgattt
     2521 tagacattat ttttctcccc agtgaatggc aattgtgcca agcatcaggc gtttgaaatt
     2581 aactcttctg aagccaacag ccaccatacg aacggacagt tcttcggcgg tcaaaaagtt
     2641 ttctgttgta tcaggtaaat attcataagc atcgcgcatg ccagaaagca ttccacccac
     2701 catggggatg atgacatgca tatgaaattt gatgaacggt gaaagtagat ttttctttgg
     2761 gcgtgtcgta tcaagaatga cgatacgtcc acccggtttg agcacacggt gttgttctct
     2821 caacgcttgt tggacgtcgg tcacattgcg catgagaaaa ccagagacaa ccgcgtcgaa
     2881 gttttttcct ttgaatggca aattcaaagc atcggcagta gagaagttca aatctccatt
     2941 ctttttgccg acacgcatca tttcaagcgt aaaatctgct gcggtgactt gggctttagg
     3001 gtactgtgtc agcgcttcgc gagctaaatc accggtaccc gttccgaggt ccagaataga
     3061 gccgtgcggg cggatcttcg tcaggctgat gacttctctc cgccaacgga tgtcttgccc
     3121 gcctgtcatc agtcggttca tcagatcgta gcgatgggcg atcttgctga acatggattg
     3181 tacatactgg gcgcgttctt gcccggttaa ttgggtcata aattctcctg aaaaacgaat
     3241 tatacacaga aggtttttga cgcacggtat aatcgcaaaa tgtcaactac atcgtattca
     3301 tttgcccggg agagccagct tgcgcttgac caagctcgcc gcattgcaga ggggaataaa
     3361 cttccttttg tagattcttt gatgcttttg cttactctgt tgcaactcaa aaattcacgt
     3421 gtgcaagcct tgttgaaatc tctcaatgtc agagtggata accttgctgc gcgcatcgtg
     3481 gcaactatca agctggaggc gaaagagatc ctccaagttt ctgaggggaa accggtctta
     3541 tcttctgaat gtgaaactgt tttagcattg gcttctgccg agatgcaact ggcgggacaa
     3601 aactcgatta gtgaatttag tttgctgttg ggcatgttac aaaacacaga aagcaaagca
     3661 ggtcaaatat tatctcagta tgcagttgca gtagaccaag cccgtgagaa aattaagata
     3721 ttcaaagaaa cccctgtttc acaaagtaat attttgcgca aagatgggtt tctgcgcaga
     3781 gcgtttcgaa atggcattag cccgatcttt attagtttgg tgttgtttac gattgccatg
     3841 gcggctttct tgtggctggg cattgggaat aatcccagag ctttcttgat tgcgttcatt
     3901 atcggcgggt ggattatctc tgtggcattg catgagtttg ggcacgccat cacagccttt
     3961 tggggtggcg atgaaagtgt tgaagataaa ggctatctca ccctcaaccc gcttaaatat
     4021 tcgaacccgg tgttgagcgt tatcattcca gttatgatgt tgctattagg tgggattggt
     4081 tttccgggtg gagcagtgta tatcaatatc ttcgcattgc gcaaaccaaa ataccgcagc
     4141 ttggtctctg cggctggacc tttagcaaat ttattggtcg ctatattgtt agcgatccca
     4201 ttcgctaact tcccattttt tttcttcttt atttatgccc ctcaaaagtt tttgatggca
     4261 ctggcttttc tggcattttt gcagattacg tctatcatca tcaatctatt gcccatcccc
     4321 ggcttggatg gcttcggtat tcttgagccg ttcttaccac gggaaatcgt tggcattgcc
     4381 agttttattc gcccatttgg ctttttgatt ttgtatctct ttgtgttcat cgattcgccg
     4441 atttcaaata tgttctggca aagtatttgg gcaattacaa atttactcag cccgagcctt
     4501 gcctattatg ccaacgaggg tttgaaactc tttttgaatt aacaacgtta tgactgaaac
     4561 aaacaaaccg aacatgtttc aaatttggtg gctcgccatc cgtccgcgca ccttgcctgc
     4621 ggcggcggca ggagttgcaa tgggcagtgc tttggcatgg catgacggtt catttcaatt
     4681 gttgcccgcg ttggcggctt tgttggtggc attgttgttg caggtcggta gtaatgttgc
     4741 taatgatgtg tttgactttg aacgcggtgc cgatacggct gaacggctcg gtcccgtgcg
     4801 tgtgactcag gctggatatc tgactccatc gcaagtgaag acgggcatgg tggtgatttt
     4861 tgcttttgca gcagtattgg gcttatatct cgccacgctt cgcggctgga ccgtgatctg
     4921 gctcggcgct gctgccattc tctccgctgt tgcctacacc ggcggacctt accctcttgg
     4981 ctatcatggc ttgggcgatg tttttgtctt tctctttttc ggtctcgctg ctgtagcagg
     5041 gacatatttt gttcaagttg gctccgtcag ccctgccgct tggtggatgg cagttccggt
     5101 tgggttgatc gtgactgcga ttttggtcgt caacaatttg cgcgacctcg aaaacgaccg
     5161 taaagcaggc aaacgcacat tagctgtccg ctttggagaa gagggcgcca aggcagaata
     5221 tctgctcttg atgttgattg cttatttgat cgtgccagtt ttggtgtgga tgaacattat
     5281 tcccattggt gcattgcttg catggttatc cctcccgatt gccattcgta ctttgagaac
     5341 ggtttttact caaaaaggaa agccactcaa tgtggcttta gcaggcactg ggcaaacagc
     5401 ctttttgttc agccttttct tttgggttgg gttacttttt tctaactgac aaacttccac
     5461 gggcaccatt ttcgctccag ccaatcgact gtgaagtaca tgccaagtcc aagcaggctc
     5521 atggcgatca cacccgcata cattgacggg taatctagta acgtgctacc tttgtaatag
     5581 atgtaatatc ccaacccata ttttgttgct gaaagttctg tgatgtacaa caccgccacc
     5641 gccgtgccaa tggattggcg tagtgcagtt agaatggctg gcaaactagc gggtaaataa
     5701 acgaaacgga agagcgcgcg cctgcccgca ccgagacttt gcaggctttg aagcaattgc
     5761 ggcgcaatac cacttgcctg atcgcggaca ag
//
LOCUS       DAOQJE010000098         5742 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_8033_length_5742_cov_7.612449, whole genome shotgun sequence.
ACCESSION   DAOQJE010000098 DAOQJE010000000
VERSION     DAOQJE010000098.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 5742)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5742)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5742
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8033_length_5742_cov_7.612449"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(72..1094)
                     /locus_tag="PLT08_03885"
     CDS             complement(72..1094)
                     /locus_tag="PLT08_03885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNE03632.1"
                     /translation="MSDKKPLLEVKNLKTYFYTEDGVVRAVDGVDFEIYPGEVLGIVG
                     ESGCGKSVTSLSIMRLIGVPGKIVEGEINFDGKNLVTASEEEMRKVRGNRISMIFQQP
                     QSALNPVFRAGDQISEVLNIHQDFGKEAGRARAVELLKQVGIPEPNRRAEAFPHELSG
                     GMSQRVMIAMALACVPDLLIADEPTTALDVTIQAQILDLMRDLRNELGSALMLITHDL
                     GVIAEMANRVVVMYAGEIVEESPVASLFDKPHHPYTQGLIGSIPVLGQRRDRLDVIPG
                     NVPNLVNLPQGCRFAPRCRARTEHNLSICTEKHPGLIQVADGHKVRCWLYEDADGHTA
                     PLKSGK"
     gene            complement(1198..1425)
                     /locus_tag="PLT08_03890"
     CDS             complement(1198..1425)
                     /locus_tag="PLT08_03890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE03633.1"
                     /translation="MNVEHPERLIVLGVAMMLFGCIAPFLMVVKVWESTFFLNFLSYG
                     MSVLGMAIGFTGMAVMRVKQKRSDDDEDKYR"
     gene            complement(1422..2411)
                     /locus_tag="PLT08_03895"
     CDS             complement(1422..2411)
                     /locus_tag="PLT08_03895"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNE03634.1"
                     /translation="MTDTQRDVLLSINDLHVWFELRRLGFGHAGYVKAVDGVTFDLHY
                     GETIAIVGESGCGKSSLMKTILGLNKPTKGSITFDGKQDVGTLEGEMLRQYRAKIGYV
                     QQDPFGALAPFMNVQRILEEPLIINGIKDPAERERRVRKVMEEVKLTPVDDFLHKFPH
                     MMSGGQQQRVVIARAIILEPKLVVADEPVSMLDASVRVEILKLLRGLQEKYKLAVIYI
                     THDLSTVRYFSERVFVMYAGNVVEKAPVDSLVHNPKHPYTTALMDATSDPDANNALTY
                     RELPSGEPPSLVNPPSGCRFHPRCPKRIENLCEVEEPSDLKSDDHLVACWLYK"
     gene            complement(2450..3412)
                     /locus_tag="PLT08_03900"
     CDS             complement(2450..3412)
                     /locus_tag="PLT08_03900"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNE03635.1"
                     /translation="MANNDNLLRLEDLVLHFGTTRGSVQAVDKVNFSLNANQAVVVLG
                     ESGCGKSSLAKAILRLLPRNVVSYSGRVYLNGNDVMQLDDEEFRQNIRWASISMVPQA
                     AMNSLNPVIRVGDQVAEPAIIHLGVSKQEAVSLVKKMFQHVGVPEDFITRYPFELSGG
                     MRQRVAIAMALVTSPSLILLDEPTSALDVLTQASIFNVLKRLKGELGISFILITHDIA
                     TSSELADSVAVMYAGQIVETSDAHRFFAEPLHPYSRMLMASVPRLRANTEPEFITGQP
                     PSLLEPPKGCRFADRCPKRFDKCSEEPPVVDVDGIQVKCWLYTS"
     gene            complement(3413..4756)
                     /locus_tag="PLT08_03905"
     CDS             complement(3413..4756)
                     /locus_tag="PLT08_03905"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNE03636.1"
                     /translation="MNNFAKSFRELLRYPSAVIGLITIIFLLVVSAYALITIPYGEAT
                     RLWRGGEDVWYQNPKYAAPAWMNLFSRVKQPVSFAVNTADGSMTKTVEVGAQDTSTIT
                     VVYSFDYAYDEPPQEMLLYFDSTFKDKLPFASVAWITPSGERIRVGDFAVDREMTFRI
                     SQDQKLMKRLKTDDVLPALFMEPKSDSTDLVKGTYQLEIIATTFEPDSDINVEFVLHG
                     KLFGLAGTDHYRRDLMLPLLWGTPIALTFGLLAALGTSLLTMIIAAVGSWYGGWVDEL
                     IQRITEINLVLPFLAILIMVGTFYSRSIWVILGVTILLSIFTGAIKSYRAVFVQSKVS
                     PYIEAARAYGTTDRRIIFSYLIPRMIPLLIPALVSSVPSYVFLEASLAVLGLGDPVLP
                     TWGKIIENAFAEGALYRGLYYWVLEPAALLMLTGLGFAMLGFALDRIFNPRLRDV"
     gene            complement(4753..>5742)
                     /locus_tag="PLT08_03910"
     CDS             complement(4753..>5742)
                     /locus_tag="PLT08_03910"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNE03637.1"
                     /translation="LIANMGGYVDTIMRNEIRDNTTQSILANPAFRQLTPEAREKLIQ
                     EKIANEEKRLGLDKPFINRSFTYLVNGLTLNLGRAVNMNSDSGSRQVRLILLERLPAT
                     LLLAGISDLALFFGSVFLALGLSRKYGSFFDKLTVALSPTSAAPPWFYGIFLILIFAG
                     VLHVLPFGGLVDSPPPATSFGYAMSVLKHIILPASSIIVSSFFISVYNWRTFFLIYSS
                     EDYVEMARAKGLPSRDIERRYILRPTLPTIITNFALLIIGMWTGFTITETVFLWPGLG
                     RTLFQAIGLYDTPVIVGSTIIYAYLLGITVFILDFVYALVDPRVKVGGDNSQQ"
BASE COUNT         1703 a         1358 c         1311 g         1370 t
ORIGIN      
        1 gacgaggtta tcgaccacca acagttctgg ctgttcttgt ttgtttattt tattcatcta
       61 agccgcgtcc tttatttacc agatttcagt ggtgcagtgt gtccatccgc atcttcatac
      121 aaccagcagc gaactttatg cccatctgct acttggatca agccaggatg tttctcggta
      181 caaatagaaa ggttgtgttc tgtacgagcg cggcaacgag gagcaaagcg gcagccttgc
      241 gggaggttga cgagattcgg aacgtttccg gggatgacat ctaaacggtc acgacgttga
      301 cccaaaactg ggatggaacc gatcaagcct tgggtgtaag ggtgatgcgg tttgtcaaaa
      361 agagaagcaa ccggagattc ttctacaatt tcgcctgcat acatcacaac gacacgattt
      421 gccatttcag cgatcacgcc gaggtcatgt gtaatgagca tcaaagcaga accgagttca
      481 ttgcgcaagt cacgcatcaa gtcaagaatc tgcgcttgaa tggttacatc aagagctgtg
      541 gtgggttcat ctgcaatcag aagatcaggg acacaagcca gtgccattgc gatcatgaca
      601 cgctgtgaca taccgccaga gagttcatga gggaaggctt ccgcgcgccg atttggctca
      661 ggaataccca cttgtttcaa cagttcaact gcacgtgcac gcccagcttc tttgccaaaa
      721 tcttgatgaa tgtttaagac ttcagagatc tggtcacctg cgcgaaaaac cggattcaaa
      781 gcagattgag gttgttggaa gatcatcgag atacgatttc cacgaacctt gcgcatttct
      841 tcttctgaag cggtaacaag gtttttgcca tcgaaattga tttcgccttc aacaatcttt
      901 ccgggaacac caatcaaacg catgatagaa agagaggtaa cgctttttcc acaccccgat
      961 tcccctacaa taccgagcac ttcgcccggg tagatctcaa aatcaacgcc atccacagca
     1021 cggactacac cgtcttcagt gtagaagtat gtttttagat tcttcacttc aagcagtggt
     1081 tttttatcgc tcattgaagg ggctcccaaa agttaggttc ggctgattat aacaaagaaa
     1141 agaagcctgc aattgccgtt cagcttttgc aggcttcttc agggtcaaaa aatgaattta
     1201 gcggtacttg tcttcatcgt cgtcagaacg tttttgtttg acgcgcatga ccgccatacc
     1261 tgtaaaacca attgccattc cgagcaccga catgccgtag gaaaggaagt tcaaaaagaa
     1321 cgttgactcc caaactttta caaccatgag gaatggcgcg atgcatccaa atagcatcat
     1381 cgctactcca agaactatga ggcgctcagg atgttcgacg ttcatttata caaccaacaa
     1441 gctacaaggt gatcatctga ctttaaatca ctaggttctt ccacttcgca gaggttttca
     1501 atgcgtttgg ggcaacgcgg atggaaacga caaccagaag gaggatttac aaggctgggc
     1561 ggttcaccag agggaagctc acggtatgtc aaagcattgt tcgcatccgg gtctgatgtt
     1621 gcatccatga gcgcagtggt atatggatgt ttcggattat gaaccaatga atcaacaggc
     1681 gccttctcca caacgtttcc agcgtacatt acgaaaacac gctccgagaa gtatcgaaca
     1741 gttgaaaggt catgggtgat gtagatcacc gccagtttgt atttttcctg caagccgcgt
     1801 aaaagcttga gaatttcaac gcggacggaa gcatcgagca tggaaacagg ttcgtctgca
     1861 acgaccagct tcggttcaag aatgatcgca cgggcaatca caacacgctg ttgttgccca
     1921 ccactcatca tgtgagggaa tttatgcaaa aagtcatcca ccggggtgag ttttacttct
     1981 tccatcacct tacgaactcg gcgctcacgc tctgcaggat ctttgatccc gttgatgatg
     2041 agcggttctt caagaatgcg ttggacattc atgaatggag ccaaggcacc aaatgggtcc
     2101 tgttggacgt agccaatctt cgcgcgatat tggcgaagca tttcgccttc caaagttcca
     2161 acatcttgct ttccatcaaa cgtaatggag cctttggttg gtttgttcaa accaagaatg
     2221 gtcttcatca agctggattt accacaacct gactcaccta caatagcaat ggtttcgcca
     2281 tagtgcaagt cgaaggtaac gccatcaact gccttcacat aaccggcgtg tccaaaaccc
     2341 aagcgacgta attcgaacca cacgtgcagg tcatttatcg agagaagcac atctctttgt
     2401 gtatctgtca tgtcttgtag acctcttttt cgattttatc ttacacaaac taggatgtgt
     2461 acaaccaaca tttgacctga atgccatcta catctacaac aggtggctct tcagaacact
     2521 tgtcaaaacg tttagggcaa cgatccgcaa aacgacaacc tttgggaggt tccaacaagc
     2581 tggggggttg tccggtaata aactctggtt ctgtattggc acgtaaacgt ggaacacttg
     2641 ccatcaacat gcgagaatac gggtggagcg gttctgcgaa aaagcgatga gcatcgctcg
     2701 tttctacaat ttgccctgca tacatcaccg caacactatc ggcaagttca ctcgatgtag
     2761 caatatcgtg cgtaataaga ataaagctaa tacccagttc gcctttgagg cgttttagca
     2821 cattaaagat gctggcttgg gtcaatacat ccaacgcgga ggtgggttca tccaatagga
     2881 tcaagcttgg agaagtaaca agcgccatag caatggcaac acgttgacgc ataccaccgc
     2941 tcaattcaaa gggatagcgg gtgataaagt cttcgggcac gcccacatgc tggaacattt
     3001 tctttaccag cgaaacagct tcttgtttgc taacccctaa atggatgatg gcaggttctg
     3061 ccacctgatc acccacacga ataacagggt tcaaggagtt catcgccgct tgcgggacca
     3121 tggagataga tgcccaacgg atgttttggc gaaactcttc gtcgtccaac tgcatcacat
     3181 cgtttccatt caagtaaacg cgtccagaat aagaaacaac attacgagga agaaggcgaa
     3241 gaatagcctt ggcgagcgag ctttttccgc agcccgactc tcctaaaaca acaactgcct
     3301 gattagcatt cagagaaaaa tttactttat ccacagcttg aacagagccg cgggtcgtgc
     3361 caaaatgtaa aaccaaatcc tcaagtcgaa gaaggttatc gttgttcgcc atttatacat
     3421 ccctcaatct tggattgaaa atacggtcaa gtgcaaagcc taacatagca aaacccaacc
     3481 cggttaacat caacaaagca gcaggctcta acacccaata atacaaacca cgatagaggg
     3541 cgccttctgc aaaggcgttt tcaataatct tgccccatgt gggcaaaaca gggtcaccca
     3601 aaccaagaac cgccaaggat gcttccaaaa atacgtaaga aggtacagag gaaaccaagg
     3661 caggaattag caaggggatc atgcgaggga tgagatagct gaaaatgatg cgtctatctg
     3721 tcgtgccgta tgcacgtgca gcctcgatat aaggtgaaac tttggattgt acgaacacag
     3781 cacggtacga tttgatcgca ccagtgaaga tactaagcaa gatagtcaca ccgaggatga
     3841 cccaaatact acgagagtag aacgtaccca ccatgatcag aatagcaagg aagggcaaaa
     3901 caaggttaat ctcagtaata cgttggatca actcatcgac ccaaccgcca taccaagaac
     3961 caacggctgc aataatcatt gtcaaaagag aggtgcccaa tgcagcgaga agcccaaagg
     4021 tcagagcaat tggggttccc caaagcaaag gcaacattag gtcacgacga taatggtcgg
     4081 tgccagcaag accaaataac ttaccatgca aaacaaattc gacgtttata tcagaatctg
     4141 gttcgaaggt agttgctatg atctcaagtt gataagtacc cttcactaaa tcggtgctat
     4201 cactcttcgg ctccataaac agagccggca agacatcatc tgtctttaat ctcttcatca
     4261 gcttttggtc ttgagaaata cggaatgtca tttcgcggtc aacagcaaaa tcgccaacac
     4321 gaattctctc accagaaggg gtgatccatg caacggaagc aaatggcaac ttatctttga
     4381 atgtgctatc aaagtaaagg agcatttctt gaggtggctc gtcgtatgca tagtcaaacg
     4441 aatagacaac cgtaattgtg gatgtatctt gagcaccaac ttcaacggtc ttggtcatac
     4501 taccatcagc cgtgttcaca gcaaacgaga cgggctgttt cacgcggcta aataagttca
     4561 tccacgcagg ggctgcatat ttggggttct gataccaaac atcttctccc ccacgccata
     4621 aacgagtcgc ttcgccgtat ggaattgtga tcagcgcata agctgaaacg accaacaaaa
     4681 atatgatcgt tatcaaacca ataacggctg agggataacg aagcagttca cggaaggatt
     4741 tggcaaaatt gttcattgtt ggctattatc tccacccacc ttcacgcggg ggtcaaccaa
     4801 ggcataaaca aagtctaaga taaacactgt aatgcctaac aaataagcat aaatgattgt
     4861 tgaaccaaca atcacaggcg tatcatacaa gccgattgcc tggaagagag ttcgtcccaa
     4921 gcctggccac aggaagacgg tttccgtaat ggtaaaacct gtccacatgc cgatgatgag
     4981 caacgcaaag tttgtaatga ttgtaggcaa agttgggcga aggatataac ggcgttcaat
     5041 gtcacgagaa ggaagacctt ttgctcgtgc catttctacg tagtcttcac tggaatagat
     5101 taagaagaat gtacgccagt tataaacact tatgaagaag gaactgacaa tgatcgatga
     5161 agccggcaga atgatgtgct taaggacact cattgcatac ccaaaagagg tggcaggcgg
     5221 aggtgagtcc actagcccac cgaatgggag gacatggagt acacctgcaa agatcaagat
     5281 cagaaagatg ccataaaacc aaggtggagc ggcagaggtc ggcgataaag ccacggtcaa
     5341 tttatcaaag aaactgccgt atttacgaga cagccctaac gctaggaata cgctaccgaa
     5401 gaacagtgcc aagtctgaga tacctgcaag caacagagta gcaggcaggc gttctaataa
     5461 gatcaaacgg acctgacgcg aaccgctatc gctattcatg ttcactgcac gtcctaggtt
     5521 aagggtcagc ccattcacca aataggtgaa agatcgatta ataaagggct tatccaaacc
     5581 cagacgtttt tcttcattgg caatcttttc ttgaatcaac ttctcgcgtg cttccggagt
     5641 cagctggcga aatgctgggt tggcaagaat agattgtgtt gtattatcac ggatttcatt
     5701 ccgcataatc gtatctacat agccgcccat attagcgatt aa
//
LOCUS       DAOQJE010000099         5732 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_8047_length_5732_cov_6.457636, whole genome shotgun sequence.
ACCESSION   DAOQJE010000099 DAOQJE010000000
VERSION     DAOQJE010000099.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 5732)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5732)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5732
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8047_length_5732_cov_6.457636"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..860)
                     /locus_tag="PLT08_03915"
     CDS             complement(<1..860)
                     /locus_tag="PLT08_03915"
                     /inference="COORDINATES: protein motif:HMM:NF012437.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNE03638.1"
                     /translation="MPNRKIEPAHLLVVDDNKVNRMALSRSLELQGHMVETAENGKEG
                     LEKFQTGTFDLMLLDIEMPVMNGFEVLEACLNDVDLRQIPIIMTSAMEELDAVVKCVE
                     LGAEDYLTKPVNPILLRARVNASLEKKRLRDEQRKLFRTFATKEVAEELLRTGFSLGG
                     QIVTASVLVADIRSFTSIAESQDPSDSIELLNQYFSMMFDAVTHNGGIINQIVGDGLL
                     AVFGAPIFYEDHRERAVKAALQMLEQLKLFNEQQLAANKTAIRIGIGIASGRMVAGYT
                     GTHNRAIYTC"
     gene            complement(865..>1215)
                     /locus_tag="PLT08_03920"
     CDS             complement(865..>1215)
                     /locus_tag="PLT08_03920"
                     /inference="COORDINATES: protein motif:HMM:NF013767.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009927 - histidine phosphotransfer kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hpt domain-containing protein"
                     /protein_id="HNE03639.1"
                     /translation="KKTWRPMPVLDMKTFNELKENTGEDFILELLNAFYEDTTKQMGE
                     MRSAVSAGDAETFRRAAHSLKSNAATFGANDLSALARELEMMGREKNLAVGNRLEALK
                     EAFDLVKRQLNELR"
     assembly_gap    1216..1315
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(1316..>5732)
                     /locus_tag="PLT08_03925"
     CDS             complement(1316..>5732)
                     /locus_tag="PLT08_03925"
                     /inference="COORDINATES: protein motif:HMM:NF024583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HNE03640.1"
                     /translation="NERLLVTPLLSGEKVNGMMAVWRKGGEPFSPADQRFQEELSLQA
                     AIAIQNANFVNEVEQRAHELEIINSIQQGLSTKLDVQSVYELVGEKVRSIFNSQAVII
                     SYYHPETETADFPYMYWKGERIYPGREPLSGFSGYVIRNKAPIFINSNLEEMAHSYGS
                     TLLAGDTFPKSLIAIPILSGDQVFGSLSIQNFEQERAFTENDLRLLSTLAASMGISLQ
                     NAKLFDESQRLLVETRQRAEELALINTILSGLDTKLGIQTVYDATGEKIREIFDAQTV
                     VLSIYDKENQLTYYPYIIENGERLYQDPLPLREDGGGFGGYVIRTKEPLLVNRDFEEY
                     SKRFQSANLGVDAEVEVVVRAGLWVPMLVGDEVKGVISLQNLEREDAFSDSDVRLLST
                     IANSIGVAIENIRLFDETQRLLKETEQRATELAILNNVSESMSRALDVNAVTHTVGNK
                     IHEIFSAEIVDILLYNSATNIVSLVFSFSNNLSYENEPPWELGEGLTSKIIITKQPLL
                     LNTAKEIEDNGAATYVTAPEDEEDIKSYMGVPIMVGDQVLGVVDVQSYKAHAFDEGSL
                     RILQILSANMGVAIQNARLFDETQRLLKETEQQNSDLSALNSVQQALVSNIDVKSIYQ
                     AVGRKLTALFNVHSAVIYTVDLDTYSMTYEYAYEQGKEWDISPKQATSLHVYITDTVK
                     TNRKSLVINKDFDEFAAQFPDFKTSRSRFPKSLCAVPILIRKNSVTGISLQHLDTEDF
                     FNDTSLRLLETIASATSVALENARLFDETQRLLNETKRRATELEILNNIGQVLTQQLD
                     VNSIIEMVGDKVRELVNEDNLGIGLYDEETKTVTAHYVTKGSERIQFPPFPINEFTQR
                     AALQGKSLVINRRSPILWKKLGSHMTAMDEIPKSVVMVPMIVGKELIGGFTIQNFEHE
                     NAYDESIITLLESIASGTATAIQNARLFEEARLARAAAEQANEAKSAFLATMSHEIRT
                     PMNAVIGMSGLLLDTELNDEQRDYVETIRGSSDTLLAIINDILDFSKIEAGRMDLEHQ
                     PFDLRECVEASLDLVTARAVEKSIDIAYIFEGDIPPAIVGDVTRLRQVITNLLSNAVK
                     FTDRGEVVLTVSSQRYKNGSAKEKVMLTFAVRDTGIGLTHEGMSRLFQSFSQADSSTT
                     RKYGGTGLGLAISKRLAEMMGGTMWAMSQGLGLGSTFMFNIKAEVADLPPAKKQIHLG
                     IQPALQNKRVLIVDDNATNRYILNMQTAKWGMVPRDTAFPAEALQWIENGDFFDIAIL
                     DMHMPEMDGLELAKHLREKQMKFPLVLFSSLGRREAGDETNLFSAYLSKPIKQSQLFD
                     TLVGLFVQDEEENKKVTTDRFKPDPQMAARHPLRILLAEDNAVNQKLALRLLEQMGYQ
                     ADVAVNGRETVQAVERKEYDVILMDVQMPEMDGLEATREIVTRWAEKHPYIIGLTANA
                     MQGDREMCLNAGMNHYIPKPIRVVELVDALFKAKS"
BASE COUNT         1331 a         1479 c         1321 g         1501 t
ORIGIN      
        1 atgcaggtgt aaatggcgcg gttatgcgtc cctgtataac ctgctaccat ccgccctgaa
       61 gcgatcccga tcccgattcg aatcgcggtc ttgttcgctg ccagttgttg ctcgttgaac
      121 agtttcaact gctccagcat ttgcaatgcg gctttcactg cccgttcgcg atggtcttca
      181 taaaagatcg gcgcaccgaa cactgccaac aagccatccc ctacgatctg attgataatg
      241 ccgccgttat gggtcacggc atcgaacatc atactgaaat attggttaag caattcaatc
      301 gaatcagaag ggtcctgact ctctgcaatg gaagtgaacg aacggatatc cgccaccaac
      361 acactcgccg ttacgatctg cccgcccaac gaaaagcccg ttcgcagcaa ttcctctgct
      421 acttctttcg tcgcaaaggt acggaataac ttccgctgtt cgtctcgcaa gcgtttcttt
      481 tcaaggctgg cgttgacgcg cgcccgcagc agaatcgggt tcaccggctt ggtcaaataa
      541 tcttcagcgc ctaactccac gcatttcacc accgcgtcca attcttccat ggctgaagtc
      601 ataatgatgg gaatttgccg taagtcaaca tcgttcaagc aagcttccag cacctcaaat
      661 ccgttcatca ctggcatctc aatatccagc aacatcagat caaaagttcc ggtctgaaac
      721 ttctccaacc cctctttgcc gttctcggcg gtctctacca tatgcccctg caactcaaga
      781 ctgcgcgaaa gcgccatccg gttaaccttg ttatcgtcca ccaccaacag atgagcaggt
      841 tctatttttc gatttggcat ccgattacct taattcattt aattgacgct tgaccaggtc
      901 aaacgcctct ttcaacgcct caagcctgtt tcccactgcc aggttcttct cacgccccat
      961 catctccaac tcacgcgcca acgccgagag gtcattcgcc ccaaacgtcg ccgcattcga
     1021 cttcaagcta tgcgctgccc gccgaaacgt ctctgcatcc cctgcgctca ctgcagacct
     1081 catctcgccc atttgcttcg tcgtatcctc atagaacgca ttgagcaatt ccaagatgaa
     1141 atcctcccca gtattttctt tcaattcatt gaaggttttc atatccaaaa caggcatagg
     1201 tctccaggtt ttcttnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1261 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnttacg
     1321 acttcgcctt gaataaggca tctaccaact ctacgacacg gatgggcttg ggaatgtaat
     1381 gattcatccc agcattcaaa cacatctcac ggtcaccttg catggcattg gcggtcagcc
     1441 caataatgta aggatgtttc tccgcccagc gtgtcacgat ctcacgcgtt gcttccaacc
     1501 cgtccatttc gggcatttgc acatccatca aaataacgtc atactcttta cgctctacag
     1561 cttgcaccgt ttcacgtcca ttcactgcca catctgcctg ataccccatc tgctccaaca
     1621 accgcaacgc caacttttga tttacggcgt tgtcttcagc gagcagaatt ctcaacggat
     1681 gccgcgccgc catttgcggg tccggtttga agcggtctgt agtcaccttc ttattctctt
     1741 cttcatcctg cacgaagagt cctaccaagg tatcgaacag ttgcgattgc ttgataggtt
     1801 tcgaaagata ggcagagaag agatttgtct catcgccggc ttcacgcctg cccaacgaac
     1861 taaacaacac caacgggaat ttcatttgct tttcacgcaa atgctttgcc aattccaagc
     1921 cgtccatttc aggcatgtgc atatcgagaa tggcgatgtc gaaaaaatca ccattttcga
     1981 tccactgcaa agcttcagca gggaaggcag tatcacgcgg aaccatgccc cacttggcag
     2041 tttgcatatt gagaatgtaa cgattggtgg cattgtcatc cacgatcaat acgcgtttgt
     2101 tttgcaatgc cggttgaatg ccaagatgaa tttgtttctt ggcggggggc aggtctgcca
     2161 cttcggcttt gatgttgaac ataaaggttg agcccaaccc caagccctgg ctcattgccc
     2221 acatcgtacc gcccatcatc tcagccaagc gtttgctaat ggcaagccct aaccccgtgc
     2281 cgccatactt gcgcgtggta gatgaatcag cttgcgagaa cgattggaag agccgactca
     2341 tgccttcgtg tgtgagaccg atacccgtat cgcgtactgc gaacgtgagc atgactttct
     2401 ctttcgcgct tccattcttg taccgttggc tggataccgt taaaacgacc tcgcctctgt
     2461 ctgtaaactt gaccgcgtta ctgagcaaat tggtgatgac ctgccgtaag cgcgtcacat
     2521 cgcccacaat ggcgggcgga atatcgcctt cgaagatata tgcaatgtcg atgctttttt
     2581 ctacagcccg cgccgtgacc aggtctagtg atgcttcgac acattcacgc aggtcaaagg
     2641 gctgatgttc aaggtccatg cgcccggctt cgatcttcga gaagtcgaga atgtcgttga
     2701 tgatggcaag caaggtatcg cttgagccgc gaatggtctc aacataatca cgctgttcgt
     2761 cgttgagttc ggtatcgagc aaaagtccgc tcatgccgat aacggcattc atgggcgtgc
     2821 gaatttcatg gctcatggtc gcgaggaagg cgctcttggc ttcgttggct tgttctgccg
     2881 cggcacgtgc caaacgcgct tcttcgaaca ggcgtgcatt ttgaatggct gtggctgtac
     2941 cagaagcaat ggactcgagc aaggtaatga tggattcgtc ataggcattc tcatgctcaa
     3001 agttctggat ggtgaagcca ccgatcagtt ctttgcccac gatcattggc accattacaa
     3061 ccgatttggg gatttcatcc atggcggtca tatgggaacc aagcttcttc cacaaaatgg
     3121 gggagcggcg gttgatgacc agactcttgc cttgcaacgc agctctttgg gtgaactcgt
     3181 tgatcgggaa cggcgggaat tgaatccgct cgctgccttt ggttacgtaa tgggctgtga
     3241 cagtcttcgt ttcctcgtca tataaaccaa tgcctaaatt atcttcattg acgagttccc
     3301 gaactttgtc gccgaccatt tcaataatgc tgttgacgtc aagttgttgg gtgagcactt
     3361 gcccgatgtt gttgagaatt tccaactcgg tggcacggcg cttggtctcg tttaagaggc
     3421 gttgggtttc gtcaaagagg cgtgcatttt cgagtgccac actggtggcg ctggcaatgg
     3481 tttctaatag acgcagcgaa gtgtcgttga agaaatcttc tgtatcaaga tgctgcaagg
     3541 aaatccctgt caccgaattt ttgcggatca agatcggtac cgcacatagc gacttgggga
     3601 aacggctgcg cgaggttttg aaatctggga attgagcggc aaactcatca aagtctttat
     3661 tgatcaccag actcttgcga ttggttttta cagtgtctgt gatgtacaca tgcaaactgg
     3721 tagcctgttt gggcgaaatg tcccattcct tgccttgttc gtaggcatat tcgtaggtca
     3781 tactatacgt atcaagatct accgtataga tgaccgccga atggacattg aagagcgcgg
     3841 ttagttttcg ccctaccgct tgataaatcg acttcacgtc aatgttggag accaacgctt
     3901 gctgtaccga gttgagagcc gaaaggtcgg aattctgctg ttcggtttct tttaagaggc
     3961 gctgggtttc atcgaataag cgtgcatttt gaatggcaac gcccatattg gcagaaagga
     4021 tttgtagaat gcgcaaactg ccttcgtcga aggcatgagc tttatagctt tggacatcta
     4081 caacgcctaa tacttggtcg cccaccataa tgggcacacc catataagac ttaatatcct
     4141 cttcatcttc aggggcggtg acataggtcg ctgcaccgtt gtcttcgatt tctttggcag
     4201 tgtttaacaa caacggctgt tttgtgataa tgatcttgga ggtgagccct tctcccaatt
     4261 cccaaggtgg ttcattctca taagaaagat tgttgctaaa gctgaatacc aaactcacga
     4321 tattcgtggc tgagttataa agcagaatgt ccacgatctc ggcactgaag atctcgtgga
     4381 tcttgttgcc cacggtgtga gtgacggcgt tgacatccaa cgcgcgcgac atcgactcgc
     4441 tgacattgtt gagaatggca agttcggtgg cgcgctgctc ggtctctttg agcaagcgct
     4501 gagtttcgtc gaagaggcga atgttctcaa tcgccacccc gatcgagttg gcaatcgttg
     4561 aaagcaagcg cacatctgag tcactaaagg catcttctct ctcaaggttt tgcagcgaga
     4621 tcacgccttt cacttcgtcg ccgaccaaca taggcaccca cagtccggca cgcaccacta
     4681 cttctacttc ggcatccaca ccaaggtttg ccgattggaa gcgtttggag tattcttcga
     4741 aatcgcggtt gaccaatagc ggctctttgg tgcggatgac gtatccaccg aacccgcctc
     4801 catcttcgcg aagcggtaag gggtcttgat agagccgttc accattctcg ataatgtatg
     4861 gataataggt gagttgattt tctttatcgt agatgctaag cacaacggtc tgtgcatcga
     4921 agatctctcg tattttttca ccagttgcgt cgtataccgt ctgaatgcca agtttggtat
     4981 ccaacccgct taagatggtg ttgatcaacg ccaattcttc tgcgcgttga cgggtttcga
     5041 ccaacaatct ttgcgactca tcgaatagct tggcattctg aagcgaaatt cccatgcttg
     5101 ctgccagggt cgaaagcaaa cgcaggtcat tttctgtaaa ggcgcgttct tgttcaaagt
     5161 tttgaatgga aagcgaaccg aagacctggt caccggagag aatgggaata gcgatcaacg
     5221 attttggaaa ggtatcccca gccaaaagag tagacccgta actgtgtgcc atttcttcca
     5281 agttcgagtt gataaagatg ggcgctttgt tacgaatgac atagccagag aaaccagaaa
     5341 gcggctcacg cccagggtag atgcgttccc ccttccaata catgtaaggg aaatcggcag
     5401 tttctgtttc ggggtgataa tacgagatga tcacagcttg cgagttgaag atgctgcgaa
     5461 ctttctctcc aaccagttca taaaccgact gtacatctaa tttggtcgat aacccttgtt
     5521 ggatgctatt gataatctcc aattcgtggg cacgttgttc cacttcattg acgaagttcg
     5581 cattttgaat ggcaatcgca gcttgcagag acaactcttc ttgaaaacgc tggtctgcag
     5641 gggagaaagg ttcgccgccc ttgcgccaaa ccgccatcat accgttgacc ttctcaccag
     5701 aaagcagcgg agtgaccaac aagcgttcat tt
//
LOCUS       DAOQJE010000100         5710 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_8106_length_5710_cov_5.787798, whole genome shotgun sequence.
ACCESSION   DAOQJE010000100 DAOQJE010000000
VERSION     DAOQJE010000100.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 5710)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5710)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5710
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8106_length_5710_cov_5.787798"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(151..1548)
                     /locus_tag="PLT08_03930"
     CDS             complement(151..1548)
                     /locus_tag="PLT08_03930"
                     /inference="COORDINATES: protein motif:HMM:NF012581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proton-conducting transporter membrane subunit"
                     /protein_id="HNE03641.1"
                     /translation="MSAPILWIAVPFVLGVLILFVLSERYAAYVGGTISTILAAIAFF
                     IPIDTALLLGNVSLKISPAIEFFGRSFELSTADGRLLTLIYGLAALWFFGTEASGTAH
                     RFVSLGLMITALLTASIAVEPFLYAALLLEMAAMLVVPLLVPLYQRPGRGVTRFIIYQ
                     TLAMPFILFSGWMLAGVEASPGDLGTIVQSGVMVTMGFAFLFAVFPLYNWIPQLIEET
                     NPYAVGFLLWALPTFTTIFALGFLDRYTWLRNTPQISNAILFSGVIMTATGGIFGALQ
                     RHLGRIFGYAAIAETGLLLIALGLRSSEVVNIAFLILIPRGLELSVWALALSIIKRKA
                     YPLKFSDVQGLARKYPVATSALIIAHLSVAGFPLLAGFPSRLALWQELSLQSLSASLW
                     VFLGLLGLLVASVRTLATLVMASDDAPWELNETWVQTFMLGIGVLGIFLLGLFPQILQ
                     PFLSSLPALFEHLIQ"
     gene            complement(1545..2132)
                     /locus_tag="PLT08_03935"
     CDS             complement(1545..2132)
                     /locus_tag="PLT08_03935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE03642.1"
                     /translation="MTLVTATMILVNRDWRISLGALALQYLAAFWLVTRHLPFAMGSV
                     KLITGWMVVAILGMTRLSLTNLDQNEQDAFYPRGAAFRITLMGIVTLVALGSTPRIEA
                     AIPGLGMPVIAGGLLLIGAGVAHLGVTNDLLRVALGLLTVLTGFEIIYAAVESAILVT
                     GLLAVVNLGLGVMGSYLLMAGSIPLISPEEEEELL"
     gene            complement(2169..3713)
                     /locus_tag="PLT08_03940"
     CDS             complement(2169..3713)
                     /locus_tag="PLT08_03940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE03643.1"
                     /translation="MLILVTCLLLFITALVLVILRATQPNARYAWLVAAGGATLAFVS
                     VFVWLAQIPFQLTLPVWKPESLFSTPILFSADGVSWALAISIAALTLAVIFTVVTRSV
                     VLNSLSWAGILVLGGMGILAVTANNPLTLLLMWALLDMTELGTQLSSVSGEKNNERVV
                     ISFATRMIGIGLLLWAYIESFTGGGMVVFQTMPPDTGVYLVTAAGLRLGVLPLHLPYA
                     ADSTLRRGFGTALRLIGAASTLSILGHIQILPTNLTPILMILTSVAAIYGGWTWLRAP
                     DELNGRPYWMIGMASLAILSALSGNATGAIAWGSALVLVGGALFLFSIQDIRLSRSLL
                     IGGWALSSLPLSLTASTWASSFNISTIFSLAAQVLLTAGYIRHALRRNGTETLDDQPG
                     WAKVVYPAGILLLLVTQILLGFIGWDGAFKFGNLIFSLLASILALGLVGGSRRFRIFN
                     PVRAHWVTTAGSGVNGLYQWLWSLYRGLAQVAQAITNALEGEGGIMWTLLFLILFISI
                     IVQGIQ"
     gene            3783..5087
                     /locus_tag="PLT08_03945"
     CDS             3783..5087
                     /locus_tag="PLT08_03945"
                     /inference="COORDINATES: protein motif:HMM:NF022702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3048 domain-containing protein"
                     /protein_id="HNE03644.1"
                     /translation="MNFGSHRPYRHGTNLWLLTLFLAVSCAEPAPASTPDPTLVPLQV
                     NYQPTPTPFQPQPGTSPDPYLAISTPQDAVPTYTPVPTKYVPPQDVSVPVDVLPAAQP
                     GVTLYNPLTGLPVSDPYFLQHRPLAIKVGNSPDYVRPQSGLTLADVVYEYYIEWGDTR
                     FIAVFYSNLYNIERVGPVRSGRYFDENITRMYHSFLMFKGADPREYDYFMGLDIKQRF
                     ISVGIGKCPPYYYGHYKRDTYNNIFFNVTLWEACVAKKGLDNGPQTISGGFFSEEVPQ
                     SPLMVNKIYNFYTEYNYSYWQYDEETHSYFRYQESKDLVGYRKVEVYEQLFDDYTKEP
                     VTADNVVVLLVPYIFTNENQAEDEVYNPSLIDYGTAYVFRDGIAIPARWNRTAIDQPL
                     LLTNLDGTPIYLKPGQTFYQVMGVTSKQIQNGDEWRFEFATP"
     gene            5128..5646
                     /locus_tag="PLT08_03950"
     CDS             5128..5646
                     /locus_tag="PLT08_03950"
                     /inference="COORDINATES: protein motif:HMM:NF024183.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HNE03645.1"
                     /translation="MTFDPVFLANLTLVLTAFAGAFLVALWISLVVWTYRDIRSRARD
                     PLVQTLSALLVAVLNLPGVLVYLILRPPRTLEEEYQRTLEEEALLQALEDLPLCPGCE
                     RRVKEDWQICPNCHTKLKKTCHNCSKLMELPWNICPYCGTPAPGVRLEATSIDDALRG
                     IKIADEQGNNDN"
BASE COUNT         1671 a         1456 c         1310 g         1273 t
ORIGIN      
        1 gtttcaaagg aggcaaggct ttcttttaac gaggtaaggg tttccttagt ctcgcgttgt
       61 tgtttatcta aaaattcaag tctttttacg atctgttcaa attccatgag gactccgttt
      121 cgttaaatta tacccttttg caaaacggga ttattgaata aggtgttcga acaaggcagg
      181 caaactagaa aggaaaggtt gcaggatctg gggaaagagt cccagcagga aaataccaag
      241 aacaccgata cctagcataa aggtctgaac ccaagtttca tttagttccc acggcgcatc
      301 gtcgcttgcc ataacgagtg ttgccaatgt gcggaccgag gcaaccaaca atcctaataa
      361 gccaagaaag acccataatg aagcacttag cgattgaagc gatagctctt gccataacgc
      421 caaccgcgag ggaaagcccg ctaaaagcgg aaaccccgcc acagaaagat gtgcaattat
      481 caatgcgctc gttgctactg gatactttcg cgctaatcct tgaacgtcac tgaacttaag
      541 cggataggct tttcgtttga tgatggatag tgccaatgcc caaacgctta actcaagtcc
      601 gcgagggatg aggatgagga aggctatatt gacaacctca gaagagcgta agccgagtgc
      661 aatcaataat agccctgtct ctgcaatagc ggcataccca aagatgcgcc cgagatgacg
      721 ttgcaacgcg ccgaagatgc cgccagtagc ggtcatgatg acacctgaga aaagaatagc
      781 gtttgaaatt tgtggtgtat tgcgaagcca cgtgtagcgg tctaaaaagc ctaaagcaaa
      841 gatggttgta aatgtaggca acgcccaaag tagaaaacca actgcatacg gattggtttc
      901 ttctatcagt tgtggaatcc aattgtagag tggaaagacg gcaaagagaa aagcaaagcc
      961 cattgtcacc atcacaccag attgaacaat cgtccccaag tctcctggac tcgcttcaac
     1021 gcctgcaagc atccagccag aaaagaggat gaacggcatg gcaagcgtct ggtaaataat
     1081 gaagcgggtc acaccacgac ccggtcgctg gtacagcgga acgagcagtg gtactactaa
     1141 catggctgcc atctccaaca agagtgcggc atacaagaat ggctctactg caatagaagc
     1201 agttagcaat gcagtgatca ttaatcccaa agagacaaaa cgatgtgccg taccagaagc
     1261 ttcggtgccg aaaaaccaga gagcagccaa gccatagatc agcgttaata accgtccatc
     1321 tgctgtggag agctcaaaac tgcgaccaaa aaactcaata gccggagaaa tcttaaggga
     1381 gacatttccc aagagaaggg cagtatcaat tggaatgaaa aaggcaattg ctgccagaat
     1441 agttgatatc gtcccaccga catatgcagc atagcgttcg cttaatacga ataggattaa
     1501 tacaccgagc acaaaaggaa cggcaatcca tagaatgggg gcactcatag cagttcttcc
     1561 tcttcttcgg gggaaataag tggaatagaa cctgccatca ataaatagga gcccatcacg
     1621 cccaacccta aattcacaac tgccaaaaga cccgtaacaa gaatggcact ttccacggct
     1681 gcataaataa tctcaaaccc agtgagcaca gtcagtagac cgagcgccac acgtaataga
     1741 tcgttagtga ccccaaggtg cgctactcct gcaccgatca gtagtaatcc gcctgcgatg
     1801 acaggcatac caagccccgg aatggcggct tcaatgcgtg gtgtggatcc taatgctacg
     1861 agcgtgacaa ttcccatcaa cgtgatacga aacgctgcac cgcgcggata aaaagcatct
     1921 tgttcatttt gatcaagatt ggtcaagctg agacgcgtca tgccgagaat agctaccacc
     1981 atccagccgg tgatcaattt gaccgacccc atcgcgaaag gcaagtgacg agtcactaac
     2041 cagaatgcgg cgagatattg aagagcaagc gcacccaagc tgatgcgcca atcacgattc
     2101 acaaggatca ttgtggctgt gacaagggtc aatgcgaccg atatccaaga aacaatatca
     2161 gagaccattt attgaatgcc ctgtacgatg attgagataa atagaattaa gaagagcaac
     2221 gtccacatga tgccgccttc gccttcaagg gcattggtaa tggcttgtgc tacttgggca
     2281 agcccacgat agagcgacca gagccattga taaagtccat tgacccctga gccagcagta
     2341 gtgacccaat gcgcacggac cgggttgaag atgcgaaacc gtctgcttcc accgaccaag
     2401 cccaaagcaa ggatggaggc gagcaaggag aaaatcaaat ttccgaattt gaaagcacca
     2461 tcccagccga taaatccaag cagaatttga gtcacaagaa gcaggagaat accagctggg
     2521 tagacgactt ttgcccaacc gggttgatcg tccaaagtct cggtgccatt cctgcgcaag
     2581 gcatgccgaa tatacccagc ggtaagtaat acctgtgccg ctaatgaaaa aatagtcgag
     2641 atgttaaaac tgctcgccca tgtgctggct gtgagcgaga gaggcagaga ggaaagcgcc
     2701 catccgccaa tgagtaagga acgacttaga cgtatatctt gaatggaaaa aagaaagagc
     2761 gcgccgccta caagcacaag cgcactgccc catgcaattg cacctgtggc attcccgctt
     2821 aacgctgaga gaatcgcaag cgaagccatg ccgatcatcc aatatgggcg accgtttaat
     2881 tcatcgggcg cgcgtagcca agtccaacca ccataaatgg cagccaccga agtaaggatc
     2941 atcaatatcg gggtcaagtt ggttggaagg atttgaatat gcccgagaat actaagggta
     3001 gatgctgcgc cgatcaaacg taaggcggtt ccaaacccgc ggcgtaaagt tgaatcagca
     3061 gcgtagggca ggtgtaatgg caatacacca agccttaacc ccgctgcagt gaccagataa
     3121 acacccgtat caggtggcat tgtttgaaaa acaaccattc cgccgcccgt aaaactctca
     3181 atatatgccc acaataatag accgataccg atcatgcgtg ttgcgaacga gatcacgaca
     3241 cgttcattat ttttctcgcc gctcactgag cttaattgtg tgcctaactc tgtcatatcg
     3301 aggagcgccc acattaagag taacgtcagt gggttattgg ctgttactgc caaaataccc
     3361 atgccaccta gcacaagaat cccagcccat gaaagcgagt ttaaaacgac tgagcgtgtc
     3421 acgaccgtga agatcaccgc aagagtcaac gccgcgatgc tgatcgctaa cgcccaagag
     3481 accccatcag cactaaaaag aatgggtgta gaaaataaac tctcaggttt ccagaccggc
     3541 aatgttagtt gaaagggaat ttgagcaagc catacaaata cactcacgaa cgcaagtgtg
     3601 gcaccaccgg ctgccaccaa ccaggcatag cgtgcattgg gttgggtagc gcgtaaaatc
     3661 accagcacaa gtgctgtaat aaaaagcaaa agacaggtaa ccaagatcaa catgggaaaa
     3721 cataattgta acagtattat gattgtcgcg ctgatacctt gtggtttggt acaatccctg
     3781 ccatgaattt tggaagtcac cgaccatatc ggcatggaac aaatctctgg cttttgaccc
     3841 tcttcctcgc cgtttcttgt gcagagcctg cccccgcctc aacccccgac ccgacgcttg
     3901 ttcctcttca agttaattat caacctacgc cgacgccttt tcaacctcag ccgggcacat
     3961 ctccagaccc ttatcttgcc atcagcaccc cgcaagacgc cgtccctaca tatacacctg
     4021 tcccaaccaa atatgttccg cctcaagatg tatccgtccc tgtagatgta ttgcctgctg
     4081 cgcaacccgg tgtgaccctc tacaacccgt tgaccgggct cccagtatca gacccatact
     4141 tcctacaaca tcgtccactc gccatcaaag tggggaattc cccagattac gttcgcccgc
     4201 aatctggctt gacccttgca gacgtggtct atgaatacta cattgaatgg ggggataccc
     4261 gttttattgc ggtcttctat agcaatctct acaacatcga gcgggtggga cccgtccgtt
     4321 cggggcgtta tttcgatgag aacatcacgc gtatgtatca ttccttcttg atgttcaaag
     4381 gtgccgaccc acgagaatat gattacttca tgggtttgga tattaaacaa cgttttattt
     4441 ctgtcggtat tggaaaatgt ccaccttact attacgggca ctacaaacgc gacacctata
     4501 acaatatctt tttcaacgtc accctttggg aagcctgtgt cgccaaaaag ggactcgata
     4561 acggacccca aaccatcagc ggcggattct tctctgaaga agttccacaa agcccgttga
     4621 tggttaataa gatttacaac ttttacaccg aatacaacta cagctattgg cagtatgatg
     4681 aagaaaccca ttcctacttc cgataccaag agtctaaaga cttggttggg tatcgtaaag
     4741 tagaagtgta tgagcaactg tttgatgact acaccaaaga gcctgttact gccgataatg
     4801 tagtcgtttt gcttgtgccg tatattttca caaatgagaa tcaagctgaa gatgaagtgt
     4861 acaatccatc cttgatcgat tacggtactg cctacgtctt ccgcgatgga attgccatcc
     4921 ctgcccgttg gaatcgaacc gccattgacc aacctctctt gctaaccaat ctcgatggca
     4981 cacccattta cctgaaaccc ggtcagacct tctatcaagt aatgggcgtt acttctaaac
     5041 aaattcagaa tggcgatgaa tggcgttttg aattcgccac gccataacca tccctatgca
     5101 ttaaaatgaa cccacctcgg agttctcatg acttttgatc ccgtctttct cgccaacctc
     5161 acgctcgtgt taaccgcttt tgcaggtgca tttcttgttg cattgtggat cagccttgtc
     5221 gtgtggactt atcgcgacat ccgttcacgt gcgcgtgacc ccttggtgca aactctttct
     5281 gctctgttag tggcagtgtt gaatttgccc ggcgttttgg tttacctcat tctgcgcccg
     5341 ccacgcacac tggaagaaga atatcagcgc acactggaag aggaagcctt gttgcaagcc
     5401 ttggaagatc ttccactttg cccgggctgt gagagacgtg tgaaagaaga ttggcagatc
     5461 tgcccgaact gtcacaccaa gctcaagaag acctgccaca actgctcaaa gttgatggaa
     5521 ctgccgtgga atatttgccc ctattgtgga acccccgctc ccggcgtacg cctcgaagcg
     5581 accagcatcg acgacgccct acgaggaatc aagatcgcag acgaacaggg aaataacgac
     5641 aattaaattc aagaccctaa agccagcttt agggtctctt ctttggagaa accatgaaaa
     5701 tcgaatcaat
//
LOCUS       DAOQJE010000101         5677 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_8182_length_5677_cov_5.469406, whole genome shotgun sequence.
ACCESSION   DAOQJE010000101 DAOQJE010000000
VERSION     DAOQJE010000101.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 5677)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5677)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5677
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8182_length_5677_cov_5.469406"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..417)
                     /locus_tag="PLT08_03955"
     CDS             complement(<1..417)
                     /locus_tag="PLT08_03955"
                     /inference="COORDINATES: protein motif:HMM:NF012780.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HNE03646.1"
                     /translation="MDPTRQPTEQEKFGRHRPLTDRDAHEINRIEELSYDLKVREVMI
                     KNVITTTSDVPLSSALELLRVNRISGLPVVSDGNLIGVISLEDIVRALQTNGLSLPVK
                     AYMSTTLVTVKATDSVVEAIKTFSEKRLGRLPVVGEH"
     gene            complement(404..757)
                     /locus_tag="PLT08_03960"
     CDS             complement(404..757)
                     /locus_tag="PLT08_03960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE03647.1"
                     /translation="MKDIIVIAEGELLTPELNTDVNIHGGCCADLMSDVLAYSRPRAV
                     LLTGLTNPQVVRTAQMADFRAIVFIRGKRPQQETIDIANQENIPLITSPMGMFELCGK
                     LHDSGLKSYEGNGSD"
     gene            complement(949..1227)
                     /locus_tag="PLT08_03965"
     CDS             complement(949..1227)
                     /locus_tag="PLT08_03965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE03648.1"
                     /translation="MSEERAKPKAFATLCGRIFRDSDPQYFPFAEYKGKKILLCTEAC
                     LGAFLADPDIFCKVHRNSDKTAARMKKEIEHLATLWSQYASERPTEEK"
     gene            complement(1224..2942)
                     /locus_tag="PLT08_03970"
     CDS             complement(1224..2942)
                     /locus_tag="PLT08_03970"
                     /inference="COORDINATES: protein motif:HMM:NF012356.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HNE03649.1"
                     /translation="MTSLKWWQTAVFYQIYPRSFADGNGDGIGDFKGITEKLDYLVSL
                     GVDALWLSPHFPSPNWDCGYDISDYTNVAPEYGTLEDFKTFLKESHKRNIRVILDLVL
                     NHTSDEHPWFLESKSSRDNPKADWYVWVDTPPNNWQSCFDGDAWTYVPERQQYYYHYF
                     MKQQPDLNWHNPEVKAAMWDAVRFWLDLGVDGYRLDAIGTIYEDANLTPHTVPMTLAE
                     LRRFSELAETEEDKKLREKYWVEMFKNQWGQPGVHELMKELRSILNEYDGDRMLVGED
                     DNIDFMGDGEDELHLVFNFPLANVNRITPEHIRKNQTERIARLNQLAKGGWACNTLGN
                     HDRSRIYTFYGDKLHDAELARLNAALTLTLRGTPFLYNGEEIGMTDYLLPHISQVKDT
                     MGSWYYHTIVTEMGVDPEEAMFRTAEMTRDKNRTPMHWSNKANAGFSPEGVETWLPIN
                     PNYKEGINVFEQEQNPSSILNYYKHLLKVRKASPALVEGDYLPLHEDATDYFAFIRKS
                     DKQTVLVVLNYSDKRLRLNFSSDQDVNGKNLHTLFTSAVRSPADHKADNLTIAPFEVF
                     IAEVTA"
     gene            complement(2995..3261)
                     /locus_tag="PLT08_03975"
     CDS             complement(2995..3261)
                     /locus_tag="PLT08_03975"
                     /inference="COORDINATES: protein motif:HMM:NF012400.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b5 domain-containing protein"
                     /protein_id="HNE03650.1"
                     /translation="MIPDRVITLAELKRNTGERGTRMWIAHLGIVYDVTECPKWRAGM
                     HEFLHFPGQDLSSEMEDAPHEDEVFKHDCVKIIGKLEEQNVSTS"
     gene            complement(3258..3989)
                     /locus_tag="PLT08_03980"
     CDS             complement(3258..3989)
                     /locus_tag="PLT08_03980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE03651.1"
                     /translation="MTNQTPIINFSPKKILIFFFSLVALLVALSIWGQHMRFFGVGDI
                     RGPIHEMFIDIMMTSFYLDYESNVPTFINALMLFIPALLLLAIGLWKSNIKDKYRFQW
                     NALAFIFFLLSIDEIASFHERLIKPMRAAVGSHGVFFFAWIIPGMAAIALFGFAFLTF
                     FLHLDKKFKLLFFFSLAVYISGVIGGEMVSGYFAANLGQKNFTYAVVASLEESVEMFG
                     CGLIIYSLLEYIKHYLPEGLTFKGS"
     gene            complement(4040..5077)
                     /locus_tag="PLT08_03985"
     CDS             complement(4040..5077)
                     /locus_tag="PLT08_03985"
                     /inference="COORDINATES: protein motif:HMM:NF012335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HNE03652.1"
                     /translation="MKAVQFTGVNQPLQMQEIPIPEIGERDILVKIKAAGVCHSDAHY
                     RAGISQVRPVPLTLGHEVAGVVEKIGANVTNVKIGDRVALHYNLSCGDCYHCSTGNDQ
                     FCEKVLMLGHYTNGGYAEYIAVPARNAVHLPEEIPFEQGATLMCASATAFHALRKSRI
                     QGGETVAIFGAGGLGQSAIQLAKAFGALEVYAVDINESKLDLAKQHGAIPVNAQKVDA
                     VAEIKRLTHNKGVNVAIEMIGLPQTMKQALQVAGVMGRVVIVGVSNKMLEVQTYTELL
                     GNEVELIGSNDHHLQELPLLIDLARRNVLDTSKVVSKTIPLDADAINAALDELERFGS
                     DVRTVIVPASS"
     gene            complement(5081..5545)
                     /locus_tag="PLT08_03990"
     CDS             complement(5081..5545)
                     /locus_tag="PLT08_03990"
                     /inference="COORDINATES: protein motif:HMM:NF014082.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HNE03653.1"
                     /translation="MIQLTPIATVKNTRIEIEDDNWGNVVSVIELDTSIPEEALLEIE
                     SFSHAEVIYYFHLVPDAKIETGSRHPRNNTAWPKVGILSQRGKNRPNRLGATIVKIVK
                     REGHQLHVQGLDAIDGTPVLDIKPVMREFLPREEIRQPEWATELMKNYWAKE"
BASE COUNT         1475 a         1270 c         1328 g         1604 t
ORIGIN      
        1 gtgttcacct accacgggta agcgccccag ccttttttcg ctgaatgttt ttattgcttc
       61 aacaacactg tctgtagctt tgacggtgac aagggttgtg ctcatgtagg ctttaactgg
      121 gagcgataaa ccatttgttt gcaaagcccg tacaatatct tcaagactaa taacaccgat
      181 caagttgccg tcgctgacga cgggcaaacc agagatgcgg ttgactctca aaagctctaa
      241 tgcgctggaa agcggcacat ccgatgtggt tgtgatgaca ttcttgatca tcacttcacg
      301 tactttcaag tcatatgaaa gttcctcgat gcgattgatc tcatgtgcat cgcggtctgt
      361 gagcggacga tgccgcccaa acttttcctg ttcagtcggt tgcctagtcg gatccattgc
      421 cttcgtagct ttttaatcct gagtcatgaa gttttccgca tagctcaaac atgcccattg
      481 ggcttgtgat caacggaatg ttttcctgat ttgcaatatc gatggtctct tgctgaggtc
      541 tcttgccgcg gataaaaacg atcgcgcgga aatctgccat ttgcgcggtt cggactacct
      601 gtgggttggt cagccccgtc agcagtacgg ctcgcggacg gctgtacgcc agtacatcgc
      661 tcattaagtc tgcacagcaa ccgccatgaa tgtttacatc agtattcagc tcgggggtca
      721 gtaattcacc ttctgcaatc acaataatgt ctttcaaatt catattcata tataaaggga
      781 tggtcctttc acctcataat aaaactctct ttaaaattat tccctactta gtctgtttag
      841 gtaagtttct tttctcatgc tcagtaggtg actattgacc tgttaagtga taaatttcac
      901 aaatatataa caaataaaaa aacgctcttt agagcgtttt tttatttgtt acttttcttc
      961 agtaggtctt tcagaagcgt attggctcca taaagtagct agatgttcga tttctttttt
     1021 catgcgagct gcagttttat ctgagttgcg gtgaacttta cagaaaatat ctgggtctgc
     1081 gagaaaggca ccaaggcaag cctcggtgca aagcaaaatt ttcttgcctt tgtattcagc
     1141 aaaaggaaaa tactgtgggt cactatctcg gaagatacgt ccgcacaggg tggcgaatgc
     1201 ctttggtttc gccctttctt cactcatgca gtgacttccg caatgaagac ttcaaatggc
     1261 gcaatggtga ggttatctgc tttatggtca gcaggggagc ggacagcact ggtgaacaag
     1321 gtatgtaagt tctttccatt cacatcttga tcacttgaga aattcaagcg taagcgttta
     1381 tcggaataat tcaatacaac caataccgtc tgtttgtctg actttctaat gaatgcaaag
     1441 tagtccgttg catcttcatg aagcggaaga taatctcctt ctaccaatgc aggtgacgcc
     1501 ttacggacct tgagcaagtg cttgtagtaa ttcaagatcg agcttgggtt ttgctcctgc
     1561 tcaaaaacat tgatgccctc tttataattt gggttgatcg gcagccaagt ttctacacct
     1621 tctggagaaa aaccagcatt tgctttattt gaccaatgca tgggggtgcg gttcttgtcg
     1681 cgggtcattt ctgctgtgcg gaacatggct tcttctgggt ccacgcccat ttcggtgacg
     1741 atggtatggt aataccagga gcccattgtg tctttaacct gcgagatatg cggcagaaga
     1801 tagtcggtca tgccgatttc ttcaccatta tagaggaaag gggtgccgcg taaagtaaga
     1861 gtaagggcag cattaaggcg agctaattcg gcgtcgtgca gcttatcgcc ataaaacgtg
     1921 taaatgcgag aacggtcgtg gttacccaat gtattgcaag cccagccacc tttggcaagt
     1981 tgatttaggc gtgcaatccg ttcggtttga ttttttcgaa tgtgttccgg agtaatgcgg
     2041 tttacatttg ccaaggggaa gttaaagaca aggtgcaact catcttcgcc gtcgcccata
     2101 aaatcaatgt tgtcatcttc gccaaccagc attcggtcgc cgtcatattc gttgagaatg
     2161 ctacgcagtt ctttcatcaa ctcgtgaacg ccgggttgac cccattgatt cttgaacatc
     2221 tcgacccaat atttttctct aagcttctta tcttcttcgg tttctgcaag ctctgagaag
     2281 cggcgcaatt cagcaagagt catgggaacg gtatgaggcg ttaggttggc atcttcataa
     2341 atggtgccga tggcgtcaag acgataccca tccacaccaa gatccagcca aaagcggacc
     2401 gcgtcccaca tagcggcttt gacttctgga ttgtgccagt tcaaatctgg ctgttgtttc
     2461 ataaagtagt gatagtaata ctgttggcgt tcgggaacat aggtccatgc atcgccatcg
     2521 aagcaggatt gccagttgtt gggcggggta tctacccaaa cgtaccaatc cgctttgggg
     2581 ttgtcgcggc tggacttgga ttctaggaac caggggtgtt cgtcagaagt atgattcaag
     2641 acaaggtcca gaatgacgcg gatgtttcgc ttgtgagact cttttaggaa ggtcttgaaa
     2701 tcttcgaggg ttccatattc tggggcaaca ttggtgtaat ctgaaatgtc gtaaccgcaa
     2761 tcccaattgg gggagggaaa atgcggcgaa agccaaagcg catccacacc gagactgacg
     2821 aggtaatcaa gtttttcggt aataccttta aagtcaccga tgccatctcc attgccatca
     2881 gcaaacgaac gcggatagat ttgataaaaa acagcagttt gccaccattt gagtgatgtc
     2941 attgtagatg taattccttt tgcattgaga gtttgaaagt ttataggcaa gaaattaaga
     3001 agttgaaacg ttctgctctt ccaacttccc aattattttt acgcagtcat gtttgaatac
     3061 ttcatcttca tggggtgcat cttccatttc gctgctcaga tcctgacctg gaaaatgcag
     3121 aaactcatgc attccggctc tccacttggg gcattcggtc acatcgtaga cgatgccaag
     3181 gtgggcgatc cacatgcgcg tcccacgttc gcctgtattg cgttttagct ctgcgagagt
     3241 gataacacgg tctggaatca tgagcctttg aacgtgagcc cttcgggaag atagtgcttg
     3301 atgtattcaa gcagggaata aatgatgagt ccacagccga acatttcaac agattcttct
     3361 aaactggcga ccacagcgta ggtgaaattt ttctgcccga ggttggcagc aaaatatcca
     3421 ctaaccattt ctccgccgat tacgccgcta atatatactg ccagcgagaa gaaaaagagc
     3481 aatttgaact ttttatcgag gtgtaagaaa aaggtgagaa aagcaaagcc gaaaagtgca
     3541 atagcagcca tgccgggaat aatccaagca aaaaagaaaa cgccgtgcga accgactgca
     3601 gcacgcatcg gtttgataag gcgttcatga aatgaagcaa tttcatctat cgaaagcaag
     3661 aaaaagatga aagcaagagc attccattga aaacgatatt tatctttgat gttgcttttc
     3721 cacaagccaa tggcaagaag tagaagtgct ggaatgaaaa gcattagggc attaatgaag
     3781 gtcggcacat tgctttcata gtccagataa aaggatgtca tcatgatgtc gatgaacatt
     3841 tcgtgtatgg ggccgcgaat atcgccaacg ccaaagaagc gcatgtgctg tccccaaatg
     3901 ctgagtgcca ctaacaacgc tacaagagag aaaaagaaaa tcagaatttt cttggggctg
     3961 aaattgatga taggagtttg gtttgtcata gatacctgtg agcgttgatt atacgtcgaa
     4021 acgctggaca gttgtcggct taggaagagg cgggaacaat gactgtgcgc acgtcactgc
     4081 caaagcgttc caactcatcg agcgcggcgt taatggcatc cgcatccaac ggaatggttt
     4141 tgctgactac ttttgatgtg tctaatacgt tgcggcgggc gaggtcaata agcaagggaa
     4201 gttcttgcaa gtgatggtcg ttggacccga tgagttccac ttcattacca agcagttctg
     4261 tgtaggtttg cacttccagc attttgttcg aaacccccac aatgaccacg cgtcccatta
     4321 cccctgcaac ttggagcgcc tgtttcatgg tctgtggaag tccaatcatc tcaatggcaa
     4381 cattgacacc tttgttatga gtaaggcgtt tgatctctgc cactgcatct actttttggg
     4441 cattgacggg aatcgcgcca tgttgtttag caaggtcaag tttcgactca ttgatatcga
     4501 cggcatatac ttcgagagcg ccaaacgctt ttgccaattg aatagcagac tgaccgagtc
     4561 cgcctgcgcc gaagattgct accgtttccc cgccttgaat gcggctcttg cggagtgcat
     4621 gaaaggcagt ggcactggca cacatcaaag ttgcaccttg ttcaaaagga atttcttctg
     4681 gtaagtgaac ggcattgcgt gctggcacgg caatatattc tgcgtaaccg ccgttggtgt
     4741 aatgcccaag catcaaaact ttttcacaaa attgatcgtt acctgttgaa cagtgatagc
     4801 aatcgccaca cgaaaggttg tagtgaagtg caactcgatc accaattttt acattggtta
     4861 cgtttgcacc gattttttca accacgcccg ctacttcatg ccctaaggta agaggtacag
     4921 ggcgcacttg tgaaatgcca gcacgataat gagcatccga atgacaaacc cccgctgctt
     4981 tgatctttac taaaatatcc cgctcgccaa tttctgggat tggaatctct tgcatttgta
     5041 acggttggtt gacaccagta aattgaactg ctttcatgta ttactccttt gcccaataat
     5101 ttttcattaa ctctgtagcc cattcaggct gacgaatttc ttctcgcggt agaaattcac
     5161 gcatgacagg ttttatatcc aagacaggag ttccgtcgat ggcgtccaat ccctgaacat
     5221 gtagttgatg accttctcgc ttcactattt taacgatagt tgctccaagc ctattgggcc
     5281 tgttcttacc ccgttggctg agaatcccta ctttgggcca agccgtgttg ttgcgcggat
     5341 gacgtgaccc ggtttcgatt tttgcatctg gaaccaaatg aaaatagtaa atgacttctg
     5401 cgtgtgaaaa actttcgatt tccagcaacg cttcttcggg tatagatgtg tccaattcaa
     5461 tgacagagac gacattaccc cagttatcat cttcgatttc gatgcgcgta ttttttacgg
     5521 tagcaatagg agttaattgg atcatattag attttccgta ccaagtcacc agttttgaac
     5581 tcgccgggtt gaattatgcg agcattgatt ccgcgcaagc gcagcgattt gttttcggag
     5641 gcactgataa attttaaagc atccagcccg aagcgag
//
LOCUS       DAOQJE010000102         5673 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_8192_length_5673_cov_4.375578, whole genome shotgun sequence.
ACCESSION   DAOQJE010000102 DAOQJE010000000
VERSION     DAOQJE010000102.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 5673)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5673)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5673
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8192_length_5673_cov_4.375578"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..265)
                     /locus_tag="PLT08_03995"
     CDS             complement(<1..265)
                     /locus_tag="PLT08_03995"
                     /inference="COORDINATES: protein motif:HMM:NF024852.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NmrA family NAD(P)-binding protein"
                     /protein_id="HNE03654.1"
                     /translation="MTNSNNTILVIGGTGMLGKPVAQQLKADGFNVRLLVRNPEKAKK
                     LLGEGYELMQGDMDNESSLRTALIGVDGVHISLKGGPTEADFER"
     gene            complement(262..483)
                     /locus_tag="PLT08_04000"
     CDS             complement(262..483)
                     /locus_tag="PLT08_04000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE03655.1"
                     /translation="MLKLILPVFLTLVIGFVLGVIGLFIAMYIGGNYAEDFVFNGVRG
                     YEATGQIGFIIGGIIGLVTGWWSLLKGKK"
     gene            complement(545..1147)
                     /locus_tag="PLT08_04005"
     CDS             complement(545..1147)
                     /locus_tag="PLT08_04005"
                     /EC_number="2.1.1.100"
                     /EC_number="2.1.1.334"
                     /inference="COORDINATES: protein motif:HMM:NF016107.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenylcysteine carboxylmethyltransferase
                     family protein"
                     /protein_id="HNE03656.1"
                     /translation="MESFLKFFLPVYLVLYFAIALFWRSFMVWKTTRVVPYVFGKTDT
                     VHDFVGLLFRLTLLSCAVVIGVVSFWTDGYPYLAPIHWLENASLDYSGLGLLFLSFFW
                     TFSAQAQMGNSWRIGIDTAHKTELVQSGIFKFSRNPVFLGMRLTLLGLFFILPNAPTF
                     VIFLIGDLLMQIQVRLEEEFLTKTHGAAYREYCTQTRRWI"
     gene            complement(1158..1877)
                     /locus_tag="PLT08_04010"
     CDS             complement(1158..1877)
                     /locus_tag="PLT08_04010"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014566.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HNE03657.1"
                     /translation="MTPLLTALIIGSAAAGIFALRPVTAKIKTVFHSNPVVNTQIKFQ
                     SLQLVLAAIVLLLTYFLNPANFTRFFRMGDVNAHISKIAWLGVTGNETWLEIAASIGL
                     FITLGTAIFMFFQLKKAGVDYRYFLFSLLWSIPFSMANAFSEEAIFRIGIVSPLYGIF
                     SVSVIVLISGIVFGAPHYFGMPSGVVGAVMAGFLGWLLALSLIETQGLLIAWAIHFAQ
                     DVVIITSMILMSKTSATVSGK"
     gene            complement(1879..2601)
                     /locus_tag="PLT08_04015"
     CDS             complement(1879..2601)
                     /locus_tag="PLT08_04015"
                     /inference="COORDINATES: protein motif:HMM:NF014566.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II CAAX endopeptidase family protein"
                     /protein_id="HNE03658.1"
                     /translation="MNAQKKSRLWWTLLAAPILALVLIVLASVYFGIITQGKDPAAIP
                     QLVANSTPYQLVALQIWLLLVLLKSMKREGLSWKDIGWKLQDSQQLWREILIGAIPGV
                     VIALLYFFVLSPVLVSLQKIWDYVPAGSVLTALGASLIPFAVADVLFAPFVEESIYRG
                     YGLTHLLGKFSQPAAVALSVFFFGILHWTGGFWYIVLVGLVAGVPFAYLRIARKNIIA
                     PFAAHLALNLIETIFIIIGIGA"
     gene            complement(2598..3002)
                     /locus_tag="PLT08_04020"
     CDS             complement(2598..3002)
                     /locus_tag="PLT08_04020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE03659.1"
                     /translation="MKILIALLRWTARITSLLALLIFILLFIEDGLPFFLLGDDDPAL
                     AFHMWALLAMLIALPLGWKWEGISAVLVLGFFIADEIALLIYGLQIGLPDGLAFPLSA
                     FSFPFLMIPISGLFYLICWLWNKKLQSQGANS"
     gene            complement(3003..4400)
                     /locus_tag="PLT08_04025"
     CDS             complement(3003..4400)
                     /locus_tag="PLT08_04025"
                     /inference="COORDINATES: protein motif:HMM:NF014573.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HNE03660.1"
                     /translation="MKIIVLNGSPKGELSVTMQSVAYLAKLHPEHEFDVVHIAQRIRH
                     FEKDRAAFDKLMEQIRASDAVLWGFPLYILIVHANYKRFIELIFERGTQNTFTGKYAA
                     ALSTSIHFFDHTAHNYIHGICDDLGMRFVDSFSADMDDLTKKEGRQQLENFGRQFFNA
                     VKNQVPTQRQYPPLVRREFTYQPVAAPAPLSTSGKKVVILHDEKDTNSNLAKMVDRCR
                     ASFTDAVTVVNIHDLNIKSSCLGCIECGSGKKCAFTDKDDYIEFYKSTVMTADILIYA
                     GQMVDRYLSSRWKMFFDRIFFNTHTPVLIDKQVALVISGPLSQNPNLLEILRGFFEFQ
                     GANLSGVVTDEYGDSAELDRLLDQLLARTLKFSAESYTHPMTYLGVGGMKVFRDDVYG
                     RLRFVFDADHRAYKRMGVYKTFPQTEFKNMLFNTFIAPIINFPPIRKQFDGRIKKEMV
                     APHKKVVEKALPTAS"
     gene            complement(4418..5254)
                     /locus_tag="PLT08_04030"
     CDS             complement(4418..5254)
                     /locus_tag="PLT08_04030"
                     /inference="COORDINATES: protein motif:HMM:NF017206.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HNE03661.1"
                     /translation="MNQTILVIGAAGFVGAPVAKQLLVDGFTVRALVRNADKARQLLG
                     NQVELVVGDFSDKATLEKALAGVDGVNISAPWQSETQVARDVIEILAKQGRKNVRVSY
                     ISGVTVLPENRGYALVEEKLKAEEILTKSGVAYTILRPNWFMDALALFVRDGRATIFG
                     KQKQAYHFLSLADFARAVSKAHQTDAAANKTFVINGPEAMLMMDALQQYCDVVHPGVK
                     AASMPTWLGNLLAGMTKSAELKDAVQMMAYFEKSPKLSAANGAEKILGASSTTLGEWL
                     KT"
     gene            complement(5356..>5673)
                     /locus_tag="PLT08_04035"
     CDS             complement(5356..>5673)
                     /locus_tag="PLT08_04035"
                     /inference="COORDINATES: protein motif:HMM:NF021886.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE03662.1"
                     /translation="PLPMDPVREACLIQIFFSHFSTNEEIAMLFETRMKEIREHLHIL
                     KTVAQAAINENAQQVGIERARQLWQITLDYGIDYYEFEMAWHEKTLKIVQNLPPIVPP
                     QNP"
BASE COUNT         1455 a         1360 c         1519 g         1339 t
ORIGIN      
        1 tgcgttcgaa gtccgcttcg gtgggaccgc ctttcaggct gatgtggact ccgtctacgc
       61 caatgagtgc ggtgcggagt gaagattcgt tatccatatc accttgcata agttcatagc
      121 cttcacccaa aagtttcttt gccttttccg ggttacgcac cagcaagcgg acattgaacc
      181 cgtccgcttt gagctgttgg gcgacaggct tgccgagcat gcccgtcccg ccgatgacga
      241 gaattgtgtt gtttgagttt gtcatttctt ccctttgagt agagaccacc agcctgtgac
      301 taacccgatt atccctccga tgataaagcc gatttgcccc gtggcttcat atccgcgaac
      361 gccattgaac acgaagtctt cggcataatt tccgccgata tacatagcga tgaacaaacc
      421 aatcactccc aaaacaaatc caatgacgag tgtgagaaaa acgggcagaa ttaactttaa
      481 catgagtgtc ctttttgatt tattggaaat cagggcagtg tttttacttg cagcccacgc
      541 cttgctaaat ccagcggcga gtttgcgtgc aatattctcg atacgctgca ccatgtgttt
      601 tcgtaagaaa ttcttcttcc aatcgtacct gaatttgcat caacaaatcg ccaattaaaa
      661 atatgacaaa ggtgggcgcg ttgggtaata tgaagaacaa accaagcagg gtcagccgca
      721 ttcccagaaa aacaggatta cgggaaaact tgaaaatacc agattgcacc agttctgttt
      781 tgtgggctgt atcaattcca atgcgccagg aatttcccat ttgtgcctga gcgctgaatg
      841 tccaaaagaa agagaggaat aacaagccca atcctgaata gtcaagtgag gcattttcca
      901 gccagtgaat cggtgcaagg tatggatacc catctgtcca aaaagaaacc actccgatca
      961 caacggcaca ggagagcagt gtcaggcgaa acagcagtcc tacaaaatca tggacagtgt
     1021 cggttttgcc aaacacataa ggaacaaccc gggttgtttt ccagaccatg aaagaacgcc
     1081 agaagagagc aatggcaaag tacaacacaa ggtaaacagg caggaaaaac ttgagaaacg
     1141 actccatggt ggtctcctta ttttccagaa acggtcgcgg aggttttact cattaaaatc
     1201 atggaggtga taatgaccac atcctgcgcg aagtggattg cccaggcgat gagcagtccc
     1261 tgcgtttcga tgagactcag cgccagcagc cagccgagaa agcccgccat cactgcgccg
     1321 accacgccgc tgggcattcc aaaataatgc ggcgcgccga agacgatgcc agaaattaga
     1381 acgatgacgg ataccgaaaa aattccatac agcggcgaga cgattccgat tctgaaaatc
     1441 gcttcttcag agaatgcatt tgccattgaa aatggaattg accagagcag actgaacagg
     1501 aagtagcgat aatccacgcc tgcttttttc aattggaaga acatgaagat ggctgtgccg
     1561 agtgtgatga acaaaccgat gcttgcggca atctccagcc aggtttcgtt gcctgtgaca
     1621 ccgagccatg cgattttgct gatatgcgcg ttcacgtcac ccatgcggaa gaaacgcgtg
     1681 aagttcgcgg ggttgagaaa atatgtcagc agtaaaacga tggcggcaag aaccagttgc
     1741 agggattgaa atttgatttg tgtattcacc acagggttgg agtgaaacac ggtcttgatt
     1801 ttggcagtga caggccgaag ggcgaaaatg cctgcggcgg ctgacccaat gatcagtgcg
     1861 gtgagaagcg gggtcatgtc aggctccgat cccgatgatg ataaaaatcg tctcgatgag
     1921 attcaacgcc aagtgcgcgg caaagggcgc gatgatattt ttacgtgcga tgcgcagata
     1981 cgcgaagggc acgcctgcga caagtccaac tagaacgatg taccagaatc cacccgtcca
     2041 gtgcagaatg ccgaagaaga aaactgaaag ggcaacggcg gcgggctggc tgaattttcc
     2101 gagcaaatgg gttaagccat agccacgata aatactctct tcaacaaaag gcgcgaaaag
     2161 cacatcggcg acggcgaagg ggatgagcga cgcgccaagc gcggtgagga cggaacccgc
     2221 ggggacgtag tcccagattt tttgcaggct aaccaaaaca gggctaagca cgaaaaagta
     2281 caacagcgca atcaccacgc caggaatcgc gccgatgaga atctcccgcc agagttgttg
     2341 actgtcttgc aatttccagc caatatcttt ccatgaaagg ccttcgcgct tcatggattt
     2401 cagcaggacg agcaggagcc agatttgcag agcaaccaat tgatagggcg tggagttcgc
     2461 aacgagttga ggaatcgccg cggggtcttt gccctgcgtg atgatgccaa aataaacgga
     2521 ggcgagcaca atcaagacca gcgcaagaat cggcgcggca agcagtgtcc accagaggcg
     2581 ggattttttt tgtgcgttca tgaattggct ccttgtgatt gcaatttttt attccacagc
     2641 cagcagatga gatagaacaa accgctgatg ggaatcatca ggaatggaaa agaaaacgcg
     2701 ctgagaggaa atgccaatcc gtcaggcagt ccgatttgca ggccatagat caacaacgcg
     2761 atttcatccg cgatgaagaa tccaagcacc agcacagcgg agattccttc ccacttccag
     2821 ccgagcggca gcgcaatcag catggcaagc aatgcccaca tgtgaaaggc aagcgcggga
     2881 tcgtcatcac caagcaggaa gaatggcaag ccatcttcga tgaagagcag gatgaatatc
     2941 agcaatgcca aaaggctggt aatacgggca gtccaacgaa gtagggcaat aagaattttc
     3001 atttaggagg cagtaggcag cgctttttca accactttct tatgtggcgc aaccatctct
     3061 ttctttatcc tcccgtcgaa ttgcttgcgg atgggaggga aattaataat gggcgcgatg
     3121 aaagtattga aaagcatgtt tttgaattcc gtttggggaa aggtcttgta aactcccatc
     3181 cgtttgtaag cgcggtgatc ggcgtcgaac acaaagcgca gtcgtccata aacgtcatcg
     3241 cggaaaactt tcataccgcc cacgccgaga taggtcatgg gatgagtata actttccgcg
     3301 gaaaatttta gggtgcgcgc cagcaactga tcgagcagac gatccaattc tgcggaatct
     3361 ccgtattcgt cggtgaccac gccgctaagg ttggctcctt ggaattcaaa gaaaccgcgc
     3421 aggatttcca gcaggttggg gttttgactc agcggtcccg atatgaccag cgccacctgc
     3481 ttgtctatca ggacgggggt gtgggtgttg aagaaaatgc ggtcgaagaa catcttccag
     3541 cgggaagaca gatagcgatc caccatttgc cccgcataga tgaggatgtc cgcagtcatg
     3601 acggtggatt tatagaattc gatgtaatcg tccttgtcag tgaaggcgca ttttttccca
     3661 gagccgcatt cgatacatcc caggcaactg gatttaatat taagatcgtg gatgttgact
     3721 acggttacag cgtctgtgaa agacgcgcga cagcggtcta ccatttttgc cagattgctg
     3781 ttggtatctt tttcatcgtg caggatgacc actttctttc cgctggtgga taggggcgca
     3841 ggcgcggcga ctggctgata agtaaattcg cggcgaacca gaggcgggta ttgacgttgc
     3901 gtgggaacct gatttttcac ggcgttaaag aactgtcgcc caaaattttc gagttgttgg
     3961 cgtccctctt ttttcgtcag gtcatccatg tcggcggaga aggagtcaac gaagcgcatt
     4021 cccaggtcgt cgcaaatgcc gtgtatgtag ttgtgcgcgg tgtggtcgaa aaaatgaatg
     4081 gaggttgaga gcgcggcggc gtactttcct gtaaaggtat tctgcgtgcc gcgttcaaag
     4141 atcaattcga taaaacgttt gtaattcgca tggacaatga gaatgtacag cgggaaaccc
     4201 cacagcacgg cgtctgaagc gcggatttgt tccatgagtt tatcgaaggc ggcgcgatcc
     4261 ttctcgaaat gccgaatccg ctgcgcgatg tggacaacat cgaattcgtg ctctgggtga
     4321 agtttggcga gatacgccac tgactgcatg gtgacgctca actctccttt ggggctgcca
     4381 ttcaaaacga tgattttcat aatgttttcc tttacttcta ggtcttgagc cattcaccca
     4441 acgtggtgga tgatgcgccg agaattttct ccgcaccgtt cgccgcgctg agtttgggag
     4501 atttttcaaa gtatgccatc atctgcacgg cgtctttcaa ctcggcgctt ttggtcatgc
     4561 cagccagcag gttgcccaac caggtcggca tggatgcggc tttgactcca gggtggacca
     4621 catcacagta ttgttggagc gcgtccatca tcaacatggc ttccggtccg ttgatgacga
     4681 aggtcttgtt ggcggcagcg tcggtctggt gcgctttcga gacggcgcgg gcaaaatctg
     4741 caagcgagag gaaatgatag gcttgtttct gcttgccaaa aatggtggcg cgtccatcgc
     4801 gaacgaaaag cgccagcgca tccatgaacc agttcgggcg cagaatggtg tacgccacgc
     4861 cgctcttggt caggatttct tccgctttga gtttctcttc aaccaaggcg tagccgcgat
     4921 tttcaggcag taccgtgacg ccagagatgt aactaacgcg cacgtttttg cgtccctgtt
     4981 tggcaaggat ttcaatcaca tcgcgagcga cttgcgtctc agattgccac ggcgcgctga
     5041 tgttgactcc gtccacgcct gccagcgctt tttcgagcgt ggctttgtcg ctgaaatcgc
     5101 caacaacaag ttccacttga ttgccgagca actggcgggc tttgtccgca ttgcgaacca
     5161 gcgcacggac ggtgaagccg tcaactagaa gttgctttgc gacgggcgct ccgacaaatc
     5221 ctgccgcgcc gataacgaga atggtttgat tcatttttac tcctttggtc gaattgaccg
     5281 atgaaaagat gcgttgaata cacccgcctt gattcaggcg gttttggtct tggtttggta
     5341 attcagtttt gcggcttacg gattttgtgg cggcactatc ggcggtaggt tttggacgat
     5401 ctttaaagtc ttttcatgcc atgccatttc gaactcgtaa taatcgatac cgtagtcaag
     5461 ggtaatctgc cagagttgac gggcgcgttc gatgccgacc tgttgtgcgt tttcattaat
     5521 cgccgcttgt gccactgttt tcaatatgtg gaggtgctcg cgaatttcct tcatacgggt
     5581 ttcaaaaagc atcgcgattt cttcgttcgt gctgaaatgc gagaagaaga tctggatcag
     5641 gcaggcttcg cggacaggat ccatcggcag ggg
//
LOCUS       DAOQJE010000103         5642 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_8256_length_5642_cov_7.399857, whole genome shotgun sequence.
ACCESSION   DAOQJE010000103 DAOQJE010000000
VERSION     DAOQJE010000103.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 5642)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5642)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5642
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8256_length_5642_cov_7.399857"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..455)
                     /locus_tag="PLT08_04040"
     CDS             complement(<1..455)
                     /locus_tag="PLT08_04040"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNE03663.1"
                     /translation="MIYNSKIQRLNYNPIFGQRRIPPMLSTPSFDEAGMQYQQNRITH
                     WNKIAQKRDSWKGLGGWYHRRLAEVYKFLVAPNQRILEIGCGQGELLAALNPSEGVGI
                     DFSEEMIVRAKQKYSNLEFHQMDAHDLSHLEGKFDIIILSDTTNDLWDV"
     gene            454..930
                     /locus_tag="PLT08_04045"
     CDS             454..930
                     /locus_tag="PLT08_04045"
                     /inference="COORDINATES: protein motif:HMM:NF019884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HNE03664.1"
                     /translation="MTVESTNEEMRELTLLEQIESDPDVNQATLAQQLGVAVGTVNWH
                     LKRLIAKGAVKVARAERKKLRYIITPEGLALRARLAVNYVENSFSVYRRTRQKVKDNL
                     AKVQKAGYDQIRIIGSGDVADICKLTCIEQGITVVTDKSAPALVLDGYRIRLEGLE"
     gene            927..1937
                     /locus_tag="PLT08_04050"
     CDS             927..1937
                     /locus_tag="PLT08_04050"
                     /inference="COORDINATES: protein motif:HMM:NF027681.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HNE03665.1"
                     /translation="MTDRHILVTGGAGYIGSLLTSELLRQNYRVTLLDSLLFGGESIL
                     PFMSHPNFHFLKTDVTEPRAIRDAVKRDWGKPDAVVHLAGIVGFPACQAVGKQVAWKY
                     NVEATKMVYGQSADLGVERFVFASTYSNYGYSEDGKPVTEETPLHPQSLYAETKIAAE
                     EYLLSQKDSACAPLLFRFATLYGISPRTRFDLIVNQFVLEAFTKRQLIIYQRGYSRSF
                     VHIRDVVRGIIIGLEAEQNKIRGQVFNLGSDTGNYSKNDIVGFVLKRMPETIVEYKNL
                     TFGGDMRDVTVSFEKIRNVLGFETTLNVDDGVREVLYALKSGLIKDPTDDRYRNAQFI
                     VQ"
     gene            1954..2925
                     /locus_tag="PLT08_04055"
     CDS             1954..2925
                     /locus_tag="PLT08_04055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-L-fucose synthase"
                     /protein_id="HNE03666.1"
                     /translation="MAENFWSNKRVVVTGGGGFLGSFVVEKLKERGATDIFIPRKKDY
                     DLTQREAIERLYVDILKSVDPKNVVIIHLAANVGGIGANREHPAEFFYDNMIMGVELM
                     HQAYKHGVGKFVATGTVCAYPKFTPVPFKEDAIWDGYPEETNAPYGLAKKMMLVQAQT
                     YRQQYGFNAIYLLPVNLYGPRDNFNLQTSHVIPALIRKAVEAGERGDKELPAWGDGSP
                     TREFLYVEDAADGIVTAAENYNGDLPVNLGSGYEISIKDLTEMVAKLTDFQGKIVWQT
                     DKPNGQPRRGLDVSRAKELFNWSAQVSFEEGMKRTIEWFKANRDKIK"
     gene            2928..3773
                     /locus_tag="PLT08_04060"
     CDS             2928..3773
                     /locus_tag="PLT08_04060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNE03667.1"
                     /translation="MTELTKHEPHTVYIKPSKGLAALNLRDLWVYRELVYFMVWRDVK
                     VKYKQTLLGMAWAVVQPVMTMLVFTFLFGKVAKLPTDGIPYPVFSFTALLPWGLFVNA
                     LNQGSRSLVAHNNMVTKIYFPRLILPMASVFSGMVDFVIAFVILVGLMFYYQVTPAWN
                     LLWTLPLFLLLALVTALGVALWLSAINVQYRDVNQALPFLSQFWLFATPVAYSSKVIS
                     EKWQVVYSLNPMAGVVNGFRWALLGTGNGPDISLWVSVGISILVFVTGLFYFKSMEKT
                     FADTI"
     gene            3773..5062
                     /locus_tag="PLT08_04065"
     CDS             3773..5062
                     /locus_tag="PLT08_04065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNE03668.1"
                     /translation="MATAISVKNIGKQYKIGAAETKFRYNMLRDVIVDTVAAPVRLAK
                     AMIGKSDRRLNQNYVWALKDVSFDLEEGKVLGIVGRNGAGKSTLLKILSRVTDPTTGS
                     VTVRGRVGSLLEVGTGFHPELTGRENIYMNGAILGMKRSEIESKFDEIVDFSEVSQFI
                     DTPVKRYSSGMYLRLAFAVAAHLEPEILVVDEVLAVGDAEFQKKCLGKMGDVAQQGRT
                     VLFVSHNMSAILRLTQEAVVLNKGQLIMRGPTQEAVDYYLSSGQSQAGERTWDADEVP
                     AASAPFMPISLKVKERSGKIVDTIRSTEKVTVEFEYKLSSPVTGLRVGLYISTMRGEY
                     VFTSFDTDTPELYEKFDSRNAGHYISRAELPADIFNEGRYMVGVNASSFGVRRYFMDE
                     NALAFNVDISGAPGTQWGEPRVGPIRPRLNWKIEKID"
     gene            5101..>5642
                     /locus_tag="PLT08_04070"
     CDS             5101..>5642
                     /locus_tag="PLT08_04070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE03669.1"
                     /translation="MPKPNPLREILGDLPFTAEIDWMLRSKNRPRKDHYNLTRLLTSL
                     PDAVKVVKPYAESAPRGKNVLFFATLHYWIEQSAYLGLVLAGMGHDVTLLTLPYSEWH
                     KEKDKFTQRQRILHTHDALKMLSPLVKHISMLDMQSSSRASHGTLHAHAASELPERIQ
                     TDIKEISLWDVQYTLMREEV"
BASE COUNT         1418 a         1301 c         1402 g         1521 t
ORIGIN      
        1 tgaacatccc ataaatcatt tgtagtatct gaaagaataa tgatgtcgaa cttgccttca
       61 aggtgcgaaa gatcgtgtgc atccatctga tggaattcaa gattcgaata cttttgcttg
      121 gcacgcacga tcatttcttc agaaaaatcg atacccacac cttctgacgg gtttaaggct
      181 gcaagtaatt caccttgtcc acaacctatt tctaggatgc gctggtttgg agcaacaaga
      241 aatttataaa cttctgctag gcgacgatga taccagccac ctaaaccttt ccaagaatca
      301 cgtttttggg cgatcttgtt ccagtgagta attcggtttt gctggtattg cattccagct
      361 tcatcaaaag atggggttga tagcattggt gggattcgtc tctgtccaaa aattgggtta
      421 taattcagtc gctgaatttt ggaattataa atcatgactg ttgaatctac aaatgaagaa
      481 atgcgagaac tcacccttct cgaacaaatc gaaagtgacc ccgatgtcaa ccaggctacc
      541 cttgcgcaac aattgggagt agccgttggc acggttaatt ggcacttaaa gcgcctcatt
      601 gcaaaagggg cagttaaagt agctcgcgcc gaacgtaaaa aactccgtta tattatcacg
      661 cctgaagggc ttgcgttgcg cgcccgcctt gcagtgaatt atgtcgagaa ttctttttcg
      721 gtatatcgcc gcacccgcca aaaggtgaag gataatctcg ctaaagttca aaaagcgggc
      781 tacgaccaaa tccggatcat cggctcgggc gacgtggcag atatctgtaa attaacctgc
      841 attgagcaag gtattacggt cgtcactgat aaatcggcac cggcgcttgt tttggatggc
      901 taccggatca gactggaagg cttggaatga cggaccgtca tatcctggtt acgggaggag
      961 cggggtatat cggctctctc ttgacctcgg aactgctccg ccaaaattat cgggtcacac
     1021 tccttgactc tttgctcttt ggcggcgaaa gtattttgcc gttcatgagc cacccgaatt
     1081 ttcattttct taagaccgat gtgaccgagc ctcgtgccat tcgagacgcc gtaaagcgtg
     1141 actggggcaa gccagatgct gttgttcacc tagcagggat tgttggcttc ccggcatgtc
     1201 aggcggtggg caagcaggtg gcgtggaagt ataacgttga ggcgacgaaa atggtctatg
     1261 ggcagtcagc agacctgggc gtggaaagat tcgtgttcgc atccacctat agtaactatg
     1321 gttactcaga agatggcaag cccgttaccg aagagactcc gcttcatcct caatcccttt
     1381 acgccgaaac gaaaatcgct gctgaagagt atttgctttc ccaaaaagat tctgcttgtg
     1441 cacccttgct ctttcgcttc gcaacattgt atggaatttc accccgcacc cgctttgatt
     1501 taatcgttaa tcaatttgta cttgaggcgt tcaccaaacg ccaactcatc atctaccaac
     1561 gcggatactc ccgctcattt gtgcatatcc gtgatgtggt gcgtggcatc attattggac
     1621 tcgaagcaga gcaaaacaag attcgcggac aggtcttcaa ccttggctca gatactggaa
     1681 attattccaa aaatgacatt gtaggctttg tgctaaaacg gatgcctgaa acgatcgttg
     1741 aatataaaaa cctgaccttc ggtggcgata tgcgtgatgt gaccgtttcg tttgagaaga
     1801 ttcgcaatgt gcttggcttt gaaaccacct tgaatgtaga tgatggtgtg cgcgaagtgt
     1861 tatacgcttt gaaatctggg ttgatcaaag acccaaccga tgaccgatac agaaatgcac
     1921 aattcattgt gcaataatgg agagatataa aacatggcag aaaatttctg gagtaataaa
     1981 cgtgtagttg tcacaggcgg aggcggtttc ctcggctcgt tcgtagtgga gaaattgaag
     2041 gagcgcggcg cgacggacat ttttattccg cgcaaaaaag actacgatct aacccaacgc
     2101 gaagccatcg aacgcttata tgtggatatc ctcaaaagcg tagacccaaa aaatgtagtc
     2161 attattcacc ttgctgcgaa tgtaggcggc atcggtgcga atcgtgaaca cccggctgaa
     2221 tttttctacg acaacatgat catgggtgtg gaactcatgc accaagccta caagcatggt
     2281 gtgggtaagt ttgttgccac tgggacggta tgcgcatacc ccaagttcac gcctgtgcca
     2341 tttaaagaag atgctatttg ggatggttat cccgaagaga ccaacgctcc ttacgggctt
     2401 gccaagaaga tgatgctggt acaagctcaa acctaccgcc agcaatatgg cttcaatgcc
     2461 atttatcttt tgccggtgaa cctctatggt ccgcgcgata attttaattt gcagacctca
     2521 catgtcatcc ctgcgcttat tcgcaaggca gtagaagctg gagaacgcgg tgacaaagaa
     2581 ctccccgctt ggggcgatgg ttcacccact cgtgaatttc tctatgtgga agatgccgcc
     2641 gacggcattg tgactgctgc cgaaaactac aacggcgatt tgccggtcaa ccttggttct
     2701 gggtatgaaa tttctatcaa agacctcacc gagatggttg caaaactcac cgacttccaa
     2761 ggcaaaatcg tctggcagac agataagcca aatggtcagc cccgccgcgg acttgacgtc
     2821 tcgcgggcaa aagaactctt caactggagc gcacaagttt catttgaaga aggcatgaag
     2881 cgcaccatcg aatggttcaa agccaatcgc gataagatca agtaaatatg actgaactta
     2941 caaaacacga acctcatact gtttatatca agccgtctaa agggcttgcg gcactcaact
     3001 tgcgagacct ttgggtgtat cgcgaattgg tttatttcat ggtttggcgt gatgtcaaag
     3061 tgaaatacaa gcagacgttg cttggtatgg cttgggcagt tgtccaaccg gtgatgacca
     3121 tgttggtctt cactttcctc tttggaaaag tcgctaaatt acctacagat ggcattcctt
     3181 acccggtctt ttcgtttacc gcccttttgc cgtggggact attcgtcaac gcacttaacc
     3241 aaggcagccg ttcgcttgtg gcgcacaata acatggtgac gaagatttat tttccacgtc
     3301 tgattctgcc gatggcttct gtcttttcgg gcatggtgga ttttgtgatt gcctttgtca
     3361 tcttggttgg cttgatgttc tactatcaag ttacccctgc ctggaattta ctctggacgc
     3421 ttccactctt cctcttgctt gctcttgtga ccgcacttgg agtggccctt tggctttctg
     3481 caatcaacgt tcaatatcgt gacgtgaatc aggcgcttcc cttcctttcg cagttctggt
     3541 tgtttgctac accagtggca tattcctcca aggtcatttc agagaagtgg caggttgtct
     3601 attccctaaa cccgatggcg ggtgtggtca acggcttccg ttgggcattg ctcggtacag
     3661 gcaatggacc ggatatctcg ttgtgggttt ctgttggaat ttcaattttg gtttttgtga
     3721 cgggtctctt ctacttcaaa agtatggaaa agacctttgc ggatacgatc taatggcaac
     3781 agcgatttca gtaaaaaata ttggtaagca atacaagatc ggcgcggcgg aaaccaaatt
     3841 ccgctataac atgttgcgtg atgtgatcgt ggatacggtt gcagcgcccg tgcgtttggc
     3901 gaaggctatg atcggaaagt ctgaccgccg cctgaaccag aattatgtct gggcattgaa
     3961 agatgtttcc tttgacctcg aagaaggcaa agtgcttggc attgtggggc gtaacggtgc
     4021 tggtaaatct accttgctga aaatcctttc ccgcgtcact gatccgacca ctggttctgt
     4081 caccgtgcgc ggacgggttg gctccctgct tgaggtagga acgggctttc accctgaatt
     4141 gaccgggcgt gagaatatct acatgaacgg cgccatcctc ggtatgaaac gctctgagat
     4201 cgaatcaaag ttcgatgaga tcgtggattt ctctgaagtg agccagttca tcgatacacc
     4261 tgtgaagcga tattcatcgg gtatgtactt gcgtttggca tttgccgtag cggcgcatct
     4321 cgaacctgaa attcttgtgg tggatgaagt gcttgctgta ggcgatgccg aattccaaaa
     4381 gaaatgtctt ggaaagatgg gtgatgttgc tcagcaggga cgcactgttt tgttcgtcag
     4441 ccataatatg tctgcgattt tgcgccttac gcaagaagcg gttgtgttga acaagggtca
     4501 acttatcatg cgtggaccta ctcaagaggc ggtagattac tacctctcct cgggacaatc
     4561 tcaagccgga gaacgcactt gggatgccga tgaggttcca gcggcgagtg cgccttttat
     4621 gccgatcagc ctcaaagtta aagaaagaag cggaaagatc gtcgatacca tccgctctac
     4681 agaaaaagtc acggttgagt ttgagtacaa gttatcctcg cctgtgactg gtttgcgtgt
     4741 gggtctgtat attagcacca tgcgcggcga gtatgtgttt acttcattcg ataccgacac
     4801 ccctgaactt tatgaaaaat ttgactcgcg taatgcgggt cattacatca gccgcgcgga
     4861 attgcccgcc gatattttca acgaaggtcg ttatatggtt ggcgtgaatg cgagctcatt
     4921 tggtgtgcgc cgctatttca tggacgagaa tgcgttggcg ttcaatgtgg atatttctgg
     4981 cgctcctggt acgcaatggg gcgagccgcg tgtgggaccg atacgcccac gattgaattg
     5041 gaagattgag aagatagatt aatcgcaggt caccttacaa aaatgacctt ttgaaaattc
     5101 atgccaaaac ctaacccact ccgcgaaatt ttgggcgacc tgcccttcac tgccgaaatc
     5161 gattggatgc ttcgctcaaa gaatcgtccg cgcaaggatc actacaacct gacgcgcttg
     5221 ctcacatcct tgccagatgc cgtgaaggtg gtaaagcctt atgccgagag cgcaccgcgt
     5281 ggaaaaaatg ttttgttctt tgccacgctt cactactgga tcgaacaatc ggcatatttg
     5341 ggtttggtgt tggcaggcat ggggcatgat gtcactttgt tgactttgcc ctattctgaa
     5401 tggcataaag aaaaagacaa atttacccaa cgtcagcgca ttcttcatac gcatgacgca
     5461 ttgaaaatgt tatctccgct cgtgaagcat atttccatgt tagacatgca atcttcatcg
     5521 cgtgcttctc atgggacgtt gcatgcccat gctgcaagtg aattgcctga aagaattcaa
     5581 accgatatca aagagatttc cctttgggat gtgcaataca ctttgatgcg cgaagaagtg
     5641 ga
//
LOCUS       DAOQJE010000104         5616 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_8315_length_5616_cov_5.888150, whole genome shotgun sequence.
ACCESSION   DAOQJE010000104 DAOQJE010000000
VERSION     DAOQJE010000104.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 5616)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5616)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5616
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8315_length_5616_cov_5.888150"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..357)
                     /locus_tag="PLT08_04075"
     CDS             complement(<1..357)
                     /locus_tag="PLT08_04075"
                     /inference="COORDINATES: protein motif:HMM:NF024313.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE03670.1"
                     /translation="MSTVQASNQSGTMIAEIEAPSLSLRDLTWRRFRRHKMAIVGLGV
                     LILLIIYSFGGAFFVTEKYSNFTNTKERLQPPSAEHPFGTDTVGRDLMARTIYGGQIS
                     IIIGITAAFVEVMMGIL"
     gene            complement(367..1401)
                     /locus_tag="PLT08_04080"
     CDS             complement(367..1401)
                     /locus_tag="PLT08_04080"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNE03671.1"
                     /translation="MTKYIVRRLLQAIPLLLLISLILFVLMQNATDPLATMGGRTPTK
                     PEDRARLTRQLGLDKPIHIQYLYWLAGNDWAKTDLDGDGIAETYGTRKGVLRGDFGTS
                     IVQTGRPVTEVIWERLPNTLMLMLTSEIIIVLGALLVGVYSALRQYSFFDNLLTGVLF
                     VGYSMPIFFIALSTMYIFSVNFKRWGLPYLPTVGMFDPKVGETPGQIILHMILPVFSI
                     SFISFAAYSRYIRSTLLEVLGQDYIRTARSKGLPKREITFIHALKNASLPIVTVVGLD
                     LPLLLGGAVVTERIFAWPGMGRLFIDGIDRGDTPVVMGILMLIAIAVVVFQILTDIVY
                     AWLDPRIRFD"
     gene            complement(1477..3147)
                     /locus_tag="PLT08_04085"
     CDS             complement(1477..3147)
                     /locus_tag="PLT08_04085"
                     /inference="COORDINATES: protein motif:HMM:NF012706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HNE03672.1"
                     /translation="MKHLKSVLFMMVISALLLAACGGGSAPADNTGGEATQAPGAGDG
                     NKTATFIFTQEFDNLNPLYTNQWFTAITYQLWSCYAWTYDDQNAPVPNLVTELPTTEN
                     GGISEDGRTLTFKLRDDIVWSDGEPITADDFVFTYEMTMDPANTVASQSPYDQMESVV
                     AADPQTVVVTFTEPFAPWAATLWKGIIPKHVLQPVFEADGNLNAAEYNRAPTVGCGPF
                     NFAEWEAGSFARFVANDNYWLGRPKLDEIFIRFVPDDASQIAALQAGEGDLGTFIAYS
                     DIPALEEAGVQMFTVFSGYNEGLYLNLGEKGHPALKELGVRQAIAYALDRESLVNDLL
                     LGRTGVAATYWDNTPFVDPSVAPYPYDPAKANELLDAAGWTDSNGDGTRDKDGVELVL
                     KYGTTTREIRKEIQAVFQQQLLEVGIGTELINAENFFDGYAEGGPAATGSLDIFEYST
                     TANFPDPHTSEWFCDQIPTDAAPDGSNWQAVCDTDLEALFTTESSQVDAAARETTFHK
                     ISKHVFDQAYWIGIYWDPDIWAVNARLTNVKLSGATPFYNIMEWDLAE"
     gene            complement(3197..4003)
                     /locus_tag="PLT08_04090"
     CDS             complement(3197..4003)
                     /locus_tag="PLT08_04090"
                     /EC_number="3.4.15.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR02069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0044265 - cellular macromolecule catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyanophycinase"
                     /protein_id="HNE03673.1"
                     /translation="MTLLMPIGGNENDKKPVVSQEFIRLAGGEKADIVVLPQPSVLED
                     TPEYYVQHFLSLGARSVKSLDFRSRAEAHLDEYVEVIQNATGIFISGGTQMRLPAIIG
                     GTNVETAIFEAYRRGIIVSGTSAGASVMSKVMIAYGKGGSTPREGIVRFTPGLGLTDK
                     IILDQHFRQRDRFGRLLYAVSAHPGLLGVGIDEDTAAIIEDEAKITVVGTGAVTVVDG
                     SELESTNVAEAEKGKPVAVSNLRIHVLTAGCSYHIPTRKAHIPNLQLVRE"
     gene            complement(4000..4674)
                     /locus_tag="PLT08_04095"
     CDS             complement(4000..4674)
                     /locus_tag="PLT08_04095"
                     /inference="COORDINATES: protein motif:HMM:NF019349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HNE03674.1"
                     /translation="MNSDSHRQLRNVVADKMRAAILDGQYKPGEWLRQERLAQELGVS
                     QMPVREALNELVNEGLIEHVPYRGARVIQFSIEDVIDLYEHRAFLEGRAAYFAAQIIT
                     DRDLTALKKIQVGMEENDAPEAVLKYRDLNRNFHKTIFTISKHKYLIRTLNQMWAAFP
                     TMLIGNFAATASHPLPARDDTDRQEHQAIMDALFAHDGTSAEQAMRNHILATREQLVS
                     FLKANQ"
     gene            complement(4684..>5616)
                     /locus_tag="PLT08_04100"
     CDS             complement(4684..>5616)
                     /locus_tag="PLT08_04100"
                     /EC_number="3.4.15.6"
                     /inference="COORDINATES: protein motif:HMM:NF015534.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyanophycinase"
                     /protein_id="HNE03675.1"
                     /translation="VFAAKKHYEYYVVGNSNNATSPTQAGTVLMGGGTDVDAAFQWMI
                     NKSGGGDFVVIRASGTDAYNPYIYGLGTVDSVETLIITSRTGASDPFVLDKINNAEAL
                     FIAGGDQADYVNYWKGTPVEDAIHNLIARNVPIGGTSAGLAILGEFSFSAANGTVDSS
                     TALGNPFGRRIALEHDFLTVPYLSSLITDSHFVTRDRMGRLVTFLARIVNDGWAPQAK
                     GLGIDEETAVVVEADGSASILGNGAAYFLQTPGTPEVCLPKTDLTYRNVSVYRISGSA
                     TFNFATWTGSGGTAYTITAENGTVTSSQPGGSIY"
BASE COUNT         1550 a         1373 c         1318 g         1375 t
ORIGIN      
        1 gagaattccc atcatcactt ctacaaaagc ggctgtgatc ccgataatga tagagatctg
       61 cccgccataa attgtacgag ccataaggtc tcgtccaacg gtgtctgttc cgaagggatg
      121 ttccgcgctc ggcggttgta gtcgttcttt ggtattggta aagtttgaat atttctctgt
      181 aacgaaaaat gctccgccaa aagaataaat aatcaaaaga ataagaaccc ccagcccaac
      241 aatcgccatt ttatgacggc ggaaacgtcg ccaagtaagg tctcgcaggg ataatgaagg
      301 tgcttcgatc tcggcgatca tcgttccaga ctgattggaa gcttgaaccg tggacataac
      361 tctctcctaa tcaaagcgaa tacgcgggtc aagccaggcg tatacaatgt ctgtgaggat
      421 ttgaaaaaca acaacagcaa tagcaatcaa catgagaatg cccataacga caggagtatc
      481 tccacggtca atgccatcaa taaaaagcct gcccataccg ggccaggcaa agatacgctc
      541 tgtcaccaca gcaccgccta gtaatagtgg caagtccaac ccaacaacgg tcacaatcgg
      601 aagagaggcg tttttgagag catgaataaa cgtgatctct cgcttgggca gcccttttga
      661 gcgcgctgtg cggatatagt cttgcccaag tacttccagc agagtgctgc ggatgtaacg
      721 gctgtaagca gcaaagctaa taaatgaaat gctaaagact ggcaagatca tgtgcaaaat
      781 gatctgcccc ggcgtttcgc caactttagg gtcaaacatc cctacagttg gaagatatgg
      841 gagcccccag cgtttgaagt tgactgaaaa aatgtacatc gtagataatg cgatgaaaaa
      901 gatcggcatg gaatacccaa caaagagcac ccccgtcaga agattatcaa agaatgaata
      961 ttggcgtaaa gcagaatata cgcccactaa caatgcccca agaacaataa taatttctga
     1021 tgtaagcatc agcatcaagg tgtttggtaa gcgttcccaa atcacctcgg tgacagggcg
     1081 tcccgtttga acgatggacg ttccaaaatc tccacgcagt acacctttgc gcgttccata
     1141 agtttcggca attccgtcgc cgtccagatc ggtttttgcc caatcattgc cagccagcca
     1201 atagagatat tgaatgtgga tgggtttatc cagccctaat tggcgggtga ggcgggcacg
     1261 atcctcaggt ttggtgggtg tacgaccacc catcgttgct aatgggtcgg tcgcattctg
     1321 catcagaaca aagaggatca gactgataag cagcaataat ggaatggctt gcaacagacg
     1381 gcgaacaatg tacttggtca tggattcacc ttcaaaaagc aggggttctg caattttatt
     1441 acagaacccc tgctaattta gtactaactc aaaatcttat tcagcaaggt cccattccat
     1501 aatgttatag aacggagtgg cgccagaaag cttgacattg gtaaggcgag cattgactgc
     1561 ccaaatatca ggatcccagt aaataccgat ccagtaagcc tgatcgaata catgcttcga
     1621 aatcttatgg aaggttgttt cacgagcggc agcatcaacc tggctgcttt cagtagtgaa
     1681 gagagcttcg aggtcagtat cgcagacagc ctgccagtta ctgccatcgg gggcggcatc
     1741 ggttgggatc tggtcacaga accattcaga agtatgagga tctgggaagt ttgcagtagt
     1801 ggaatattcg aagatatcca acgaaccggt cgcagccggt ccaccttcag catagccatc
     1861 gaagaagttt tcggcgttga tcaattcggt gccaatgcct acttcaagta actgctgttg
     1921 gaacacagcc tgaatttcct tgcggatttc acgggtagtg gttccatact tcaagacgag
     1981 ttcaacaccg tccttatcgc gggtgccgtc accattagag tcggtccagc cagcggcatc
     2041 gagaagttcg ttcgccttgg cagggtcata aggatacggg gcgacagacg ggtcaacgaa
     2101 cggagtgtta tcccagtatg ttgcagccac gccagtgcga cccaacagta aatcattgac
     2161 aagggattcg cggtcaagtg cataagcaat tgcctggcga acacccaatt ccttcagcgc
     2221 agggtgacct ttctcgccaa ggttcaggta caaaccttcg ttatagccag agaagacggt
     2281 gaacatttgc acgcctgctt cttcaagggc gggaatgtct gagtaggcaa tgaacgttcc
     2341 gagatcacct tcaccagcct gaagagcagc gatctgagaa gcatcatcgg gtacgaagcg
     2401 aatgaagatt tcatccaact tggggcgtcc gagccaatag ttatcattgg caacaaaacg
     2461 ggcaaaagaa ccagcttccc attcagcaaa attgaatgga ccacagccga cagtcggtgc
     2521 gcggttgtat tcagcggcat tcaagttgcc atctgcttca aacacaggct gaagaacatg
     2581 ctttggaatg atgcctttcc aaagggttgc agcccaaggc gcaaacggtt cggtgaaagt
     2641 caccaccaca gtttgcgggt ctgcagcgac aacgctttcc atctggtcat agggactctg
     2701 agaagccacg gtatttgcag ggtccatggt catttcgtag gtgaatacga aatcatcagc
     2761 agtgataggc tcaccatcag accatacgat atcgtcgcgc aacttgaagg tcagggtgcg
     2821 accatcttca gagatgccgc cattttcggt ggtcggcaat tcagtcacca aattcggaac
     2881 aggggcgttt tgatcatcat aggtccatgc atagcagctc cagagctgat atgtaatggc
     2941 agtgaaccac tgattggtgt agagcgggtt cagattatca aactcctgag taaaaatgaa
     3001 ggtggcggtc ttgttgccat caccagcacc aggcgcctgt gttgcttcgc caccggtatt
     3061 atccgccggg gcagagccac caccacacgc tgcaagcagc aatgcagaaa tgaccatcat
     3121 gaacagaaca gactttagat gtttcatgct tttcttctcc tttgtgttat ttgaaaacat
     3181 gtgaaccgtt cacatgctac tcacgaacca attgcaggtt ggggatatgt gctttgcgtg
     3241 ttggaatatg atatgaacaa cctgccgtta aaacatggat tcttagattg ctgaccgcaa
     3301 cgggttttcc tttttctgct tctgccacat tggtggattc aagttcgctt ccatctacaa
     3361 cggtcactgc tcctgtaccc acaaccgtga tcttcgcctc atcctcaatg atcgcagctg
     3421 tatcctcgtc aatgccgacg ccgagcaaac ccggatgtgc cgaaactgca tagagcaaac
     3481 gtccaaaccg atcacgctgc cgaaaatgtt gatccaaaat aattttatcg gttaatccca
     3541 agccaggggt aaaccgaact atcccttcgc gcggcgtgga accgcctttt ccataagcaa
     3601 tcatcacctt agacattaca gatgccccag cacttgttcc cgaaacaatg atgccacgcc
     3661 gatatgcctc aaagattgct gtttcaacgt ttgtcccacc gatgatggca ggcaaacgca
     3721 tttgggtgcc gccagaaata aaaatacccg ttgcattttg aatgacctca acatattcat
     3781 ccaagtgcgc ctcagctcgt gagcgaaaat ctaaactctt cactgaacga gcaccaagac
     3841 ttaagaaatg ctgaacataa tactctggag tatcctctaa aaccgacggt tgtggaagca
     3901 ccacgatatc ggctttttcc cctcctgcaa ggcggataaa ctcttgcgaa acgactggtt
     3961 tcttgtcgtt ttcgtttcca ccaatcggca ttaacagcgt cattgattcg cctttaagaa
     4021 tgaaaccaac tgctcacgag tagcgagaat atgatttcgc atggcttgtt ccgcagaagt
     4081 cccgtcatgt gcaaataaag catccataat cgcttgatgc tcctgccgat ctgtatcatc
     4141 gcgggcaggc aaagggtgcg atgctgtagc tgcgaagttg ccaatcaaca tcgttgggaa
     4201 agcagcccac atttgattta gggtacggat taagtattta tgtttactaa tcgtaaaaat
     4261 agtcttgtga aaatttcggt taaggtcacg atacttcaat acagcttctg gagcatcatt
     4321 ctcttccatt ccaacctgta tcttttttag agccgttaag tcacggtctg taataatctg
     4381 tgctgcgaaa tatgctgcac gcccttcaag aaaggcacga tgttcataca ggtcaattac
     4441 atcttcaata gaaaactgaa tgacacgcgc gcctcgataa gggacatgtt cgatcagccc
     4501 ttcattgaca agctcattta gagcttctcg cacaggcatt tggcttactc ccaactcctg
     4561 tgcgaggcgc tcctgacgca gccattctcc gggtttgtat tgcccatcaa gaatggctgc
     4621 acgcatttta tctgcgacaa cgttccgtag ctgtctgtga gaatctgaat tcatagctcc
     4681 gtcttagtaa atggatccac cgggttggga agatgtcaca gtgccatttt cagcagtgat
     4741 cgtatatgct gttccgccgc tgcctgtcca tgttgcgaag ttgaatgtgg ctgagccgct
     4801 aatgcgataa acagaaacat tacgataggt taggtcggtt ttaggtaggc aaacttcagg
     4861 cgtgccagga gtttgaagga agtatgcagc tccgtttcca agaatagagg cgctcccgtc
     4921 agcttcaaca actacggcag tttcttcatc gattcccaac ccttttgcct gtggtgccca
     4981 tccatcgttg acgatacgag caaggaatgt aacgagtcga cccatacggt cacgggtaac
     5041 aaagtgacta tccgtgataa gactgctcaa ataagggacg gtaagaaaat catgctcgag
     5101 agcaatacga cgaccaaagg ggttgcctaa agccgtggaa gagtctactg tgccatttgc
     5161 tgctgagaaa gaaaactctc ccaaaatggc aagcccggca cttgtgcctc ctataggcac
     5221 gttccgtgca atcagattat gaatcgcatc ttccactgga gtccctttcc agtagttaac
     5281 ataatcagcc tggtcgcctc ctgcaataaa cagagcttca gcattattga tcttatccaa
     5341 tacaaagggg tcacttgcgc cagtacgtga tgtaataatg agagtctcta cagagtcaac
     5401 cgtgcctaaa ccataaatat aaggattata ggcatctgta cctgaagcgc gaatgacgac
     5461 aaaatctccc ccacctgatt tattgatcat ccattgaaat gctgcatcaa catcggtgcc
     5521 accgcccatg aggaccgttc cggcttgagt aggtgaagtg gcattgtttg agttaccaac
     5581 aacatagtat tcataatgtt tttttgctgc gaaaac
//
LOCUS       DAOQJE010000105         5580 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_8402_length_5580_cov_5.848145, whole genome shotgun sequence.
ACCESSION   DAOQJE010000105 DAOQJE010000000
VERSION     DAOQJE010000105.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 5580)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5580)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5580
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8402_length_5580_cov_5.848145"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..939
                     /locus_tag="PLT08_04105"
     CDS             <1..939
                     /locus_tag="PLT08_04105"
                     /inference="COORDINATES: protein motif:HMM:NF012457.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 1 glycosylhydrolase"
                     /protein_id="HNE03676.1"
                     /translation="TEEIVPLFEKYVRKMVDSLKEYTNLWVTINEPNVYALSGYVTGD
                     FPPGKKDLKQAMPVIANMVRGHAAAYRAIHELQPEARVGYALHFRPMVPRLSWSPLDR
                     LMRNIRYEGVNMGFPTAISTGVMKTPIGNFNIPEAKGTQDYLGLNYYSVDTVWFDLAK
                     ADQMFSNSGYPKDADMSDTNFLANIPHGIYDSIKWIVRTYPNLPIIVTENGVEDFDDH
                     MRPRYLVQHLHEVWRTVNFNWPVKGYFHWSLVDNFEWERGWTQRFGLWGLDLDTQKRI
                     RRPSVDLYAEICKENGISSEMVQKYCPEVFEKLFPS"
     gene            complement(1042..1944)
                     /locus_tag="PLT08_04110"
     CDS             complement(1042..1944)
                     /locus_tag="PLT08_04110"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNE03677.1"
                     /translation="MITYRRATPADNFTTFTIFRQSLEDFSKRTGVMAITGGNDPQKM
                     DELWESRRSFWEHLTNTSDNYWIAEKDGEAIGYARSILRGNHRELTEFFVVPGNQSVG
                     VGKELLQRAFPDDVPHRTIIATPDIRAQTRYLKAGVYPFMTEFYFERTPEPQIFETDL
                     VIELAEASPASSDDLGRIDLSILGFRRDIDHQFLTSDRKLYFYKRNGQVVGYGYINRD
                     LYGPFALLEKEDFPAVLAHAENEAHKMGASVVGFEVPTINSSAISHLLNRGYRLEGFL
                     GSIMSEKPFGRFEHYVLTSPPYFL"
     gene            complement(1980..2729)
                     /locus_tag="PLT08_04115"
     CDS             complement(1980..2729)
                     /locus_tag="PLT08_04115"
                     /inference="COORDINATES: protein motif:HMM:NF020296.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HNE03678.1"
                     /translation="MSRAKFQKYHREVAQALTSIGNPAFGKAVQKDRGSHLKHLGIKF
                     PELRRRVKQGFSFYALPEEQILEIWDALWQTSPYGDVLFAALEYYIPIVKKQVPANLW
                     GVVKGWSLRIDNWCHSDALCSVYSRILEGNQKEVYPQLLEWNQAQSEWLRRISLISLI
                     HYSGKNAVFMPPKKVLPLVSNCLDDERYYVQTAVGWVLREMGQMYQGEIQKYLEIHVE
                     RLSSPAFSRSIERLSTKERTHLQGIRKRRVA"
     gene            complement(2761..3930)
                     /locus_tag="PLT08_04120"
     CDS             complement(2761..3930)
                     /locus_tag="PLT08_04120"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013434.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HNE03679.1"
                     /translation="MTQTYDAIVIGAGVMGASLAFHFAERGLKVAILERKVTASGATG
                     HSSGLVRMHYDLAAESHLTFASYKNYFSIWKERVGGECGFQQTGFLQIAKREHEDKLR
                     GNVANQQKIGINTSVISVDEVRKLFPDLVTEYFDYAAYEPDSGYADATLTTNSFLDSA
                     KRNGATLIQNCEVTAIHVSGVKVTGVSTTQGDFSAPIVVNAAGPWAKHVAALAGIEVP
                     LVTWTHDVAFLHRPPTLGKIPAVIDDVINCYFRPEGSALILAAGEDESLRGEAPDAED
                     QTPTPTFLDKLIDQMVQRIPKIEESGLHSVHVGRDGITPDQRAIYSGTDLGGFYLACG
                     LSGTGFKTSPAAGASMVELILDGTPKTVDITPFRFSRFADGTLLEGEYGYGNIWK"
     gene            complement(4007..4432)
                     /locus_tag="PLT08_04125"
     CDS             complement(4007..4432)
                     /locus_tag="PLT08_04125"
                     /inference="COORDINATES: protein motif:HMM:NF016001.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family deazaflavin-dependent
                     oxidoreductase"
                     /protein_id="HNE03680.1"
                     /translation="MSAKHNPFIKLFWSIHLKLYLWSSGRIGNTIRGLPVLLLTTKGK
                     KTGQLRTKALMYLPYGDAFVVIASNLGKDNHPAWWLNLQADSTATVQVKDARFTVRAR
                     EAEGDEREKLFQALAQKTSDYEQYRSWTSRRIPVVILER"
     gene            complement(4434..5468)
                     /locus_tag="PLT08_04130"
     CDS             complement(4434..5468)
                     /locus_tag="PLT08_04130"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HNE03681.1"
                     /translation="MIRKLRILTLLSIFLHACVSASASTPPTETLTPPIPTASLTPSP
                     IPPAPETPQPTVTIPAPQPISRVFIVTFDGLRPDAIDAAGMTNVQEMMKNGAYTLTAQ
                     TIMPSTTLPAHSALVTGTCPAKNTVRWNEYVPQNGYALGTDIFDLTRSVGLRTVLVVG
                     KEKLRQITESTDFFGWVDNTDEAKDKTTLDNMALEQIDLGFGLMMVHFPDGDVVGHEY
                     GWLSKQQLSAYKKNDQSFGRFISAMKKNGYYDGTLFIITSDHGGALTDPPHGTNIPEA
                     MTIPWIISGPQIVPGELKTPVYIFDTAPTVAFALGLPLQAEWDGTPVYEAFGLPVDPL
                     HQGGCPGLTP"
BASE COUNT         1394 a         1318 c         1470 g         1398 t
ORIGIN      
        1 accgaagaga tcgtgccgtt gttcgagaaa tatgtgcgca agatggtgga ttctttgaag
       61 gaatacacca acctatgggt gacgatcaat gaaccaaatg tctatgcatt gagcggatat
      121 gtaacgggag atttcccgcc tgggaaaaaa gacttaaagc aagccatgcc agtcatcgcc
      181 aatatggtgc gcggacatgc cgccgcctac cgtgccattc atgagttgca gccagaagca
      241 cgggtggggt atgccttgca tttccgcccg atggtgccgc gtttgagttg gtcaccttta
      301 gaccggctaa tgcgcaatat ccgctatgaa ggcgtgaata tgggcttccc cactgcaatc
      361 tctaccggtg tgatgaagac tccaatcggc aatttcaaca tccctgaagc caaagggacg
      421 caagattatc tgggcttgaa ctattactcc gtggatacgg tttggttcga tcttgcaaag
      481 gcagaccaga tgttttcaaa cagcggctac cccaaagatg ccgatatgag cgacacgaat
      541 tttctcgcga atattccgca tggcatttac gactccatca aatggatcgt gcggacctat
      601 ccaaacctgc ccatcatcgt cactgaaaac ggcgtggaag atttcgacga tcacatgcgt
      661 ccgcgctatt tggtgcagca tttgcatgaa gtttggcgca cggtcaattt caactggcca
      721 gtgaagggat acttccactg gtcactggtg gataactttg aatgggagcg cggttggaca
      781 caacgcttcg gtctgtgggg cttggacttg gatacccaga aacgcatccg ccgcccgtct
      841 gtagatctgt atgctgagat atgtaaagag aatgggattt caagcgagat ggtgcagaag
      901 tattgccccg aagtgtttga gaagttgttt ccttcgtaag ttttattaat gtcaccctga
      961 gcggaccgaa gggtctctac ctatgggtca gagactcttc ggtcgcgaag agcacgcttc
     1021 ctcagagtga catagcggta ctcacaaaaa atagggcgga ctggtcagaa cgtaatgttc
     1081 aaatcgtcca aatggttttt cagacatgat cgagccaaga aagccttcga ggcggtatcc
     1141 gcgattgaga agatgactga tggcagatga attgatggtg ggtacttcaa agccgacaac
     1201 agacgcgccc atcttgtgcg cttcattttc tgcatgagcc agcacggcag gaaaatcttc
     1261 cttttcaagc aacgcaaaag gaccatataa atctcggttg atatacccgt aacctacgac
     1321 ttgtccgtta cgtttgtaga aatacaactt acggtcactg gttaggaatt ggtggtcaat
     1381 gtcgcggcgg aaaccaagga tcgagaggtc gattctgcct aagtcgtcgg aagaggcggg
     1441 cgaagcttct gctaattcaa taacaaggtc tgtttcgaaa atctgcggct cgggagtacg
     1501 ctcaaaataa aattcggtca tgaagggata tacgcccgct ttgagataac gcgtttgagc
     1561 gcggatatcg ggagtggcaa tgatggtgcg atgcggcaca tcatcgggga aggcgcgctg
     1621 gagcaattcc ttgcccacac ccacagactg attgcccggc acaacaaaga actcggtcag
     1681 ctcgcgatga tttccgcgca agatgctgcg ggcgtagcca atggcttcgc catctttttc
     1741 ggcgatccaa taattatcgc tggtgttggt gagatgctcc caaaaactgc gacggctttc
     1801 ccaaagctca tccatttttt gcgggtcatt gccgccagtg atcgccatca cgccagtgcg
     1861 tttactgaaa tcttccaacg actgacgaaa gatagtgaag gtagtgaaat tatctgcggg
     1921 ggtggctctg cggtaagtga tcatagttgg acattttagc agagtttgat tttatgcgat
     1981 catgccacgc ggcgttttcg aatgccttgt aagtgtgttc gttctttcgt gctcaaccgt
     2041 tcaatggacc ttgaaaaagc aggcgaactt aatcgttcta cgtgaatttc aagatatttt
     2101 tggatttcgc cttgatacat ttgccccatt tctcgcaaaa cccagccaac cgccgtttga
     2161 acatagtagc gttcatcatc taaacaattc gacaccaacg gcaggacttt tttcggcggc
     2221 atgaagactg catttttgcc gctgtaatga ataaggctga tgagagaaat gcgcctcaac
     2281 cattcacttt gggcttgatt ccactctagg agttggggat acacttcttt ttgattgccc
     2341 tctaaaatgc gcgaatacac cgaacaaagc gcatcgctat gacaccaatt gtcaatgcgt
     2401 aaagaccaac ctttgacgac gccccacaga ttggctggaa cttgtttttt cactatggga
     2461 atatagtatt caagcgcggc aaaaagcaca tcgccatagg gggaagtttg ccatagcgca
     2521 tcccaaatct ctagtatttg ttcttctggc aaagcataaa aagaaaaacc ttgtttcact
     2581 ctccgccgca actcgggaaa tttgatacca agatgcttaa gatgcgagcc gcggtctttc
     2641 tgcacagctt tgccaaacgc tgggttgcca atggatgtta gagcctgcgc cacttcgcgg
     2701 tgatacttct gaaattttgc gcggctcatc aggcaatact ctcaatatgt gacaaagaaa
     2761 ttacttccaa atatttccat acccatattc gccttcgagc aaagtcccat cggcgaaacg
     2821 gctaaaccgg aacggagtga tatccacagt tttgggtgtg ccatccaaaa tcaattccac
     2881 catgctagca ccagcagcag gtgacgtttt gaaaccagtg ccactcaaac cacaagcgag
     2941 ataaaacccg cccaagtctg ttccgctata gatggcacgt tgatcgggcg taatgccatc
     3001 acgcccaaca tggacagaat gcaagccgct ctcttcgatc ttcggaatgc gctgtaccat
     3061 ttgatcaatg agcttatcaa gaaacgtggg tgtgggcgtt tggtcttcag cgtctggcgc
     3121 ttcgccacgc aatgattcat cttcaccagc ggcaaggatg agcgcgctgc cttccgggcg
     3181 gaagtagcag ttgatgacgt catcgatcac agcggggatt tttcccaatg tgggcgggcg
     3241 atgaagaaag gcgacatcgt gagtccatgt caccaatggg acttcgattc ctgccaacgc
     3301 cgcaacatgt ttcgcccacg gtcccgccgc attgacgacg attggggctg agaagtcgcc
     3361 ttgggtcgtg cttacgccgg tcacctttac gccactgaca tggatggcgg tcacttcgca
     3421 gttttgaatc agcgtggcgc cgtttcgttt tgcagaatca agaaaagagt tggtggtgag
     3481 cgtcgcatcg gcatagccgg aatccggctc ataagcagca tagtcaaagt attctgtcac
     3541 aaggtctgga aaaagtttgc gaacttcatc caccgaaata accgaggtgt tgataccaat
     3601 cttttgttgg ttcgccacat tgccacgcaa tttgtcttca tgctcgcgtt tggcaatttg
     3661 caagaaaccg gtctgttgaa aaccacattc gccgcctacg cgctctttcc aaatgctgaa
     3721 atagttttta tagctggcaa aggttaggtg tgactcagca gcgagatcgt aatgcatgcg
     3781 gacaagcccg ctggagtgcc cggtggcacc agaggctgtc actttgcgtt caagaatggc
     3841 gactttcagt ccgcgctcgg cgaagtggaa agcaaggctg gcgcccatca caccggcacc
     3901 aatcacaatg gcatcgtagg tttgagtcat agaagagctc cttttgccgc gaattgtgcg
     3961 gttgagtttt ttggttgcga ggaattttag cagagtgggg cgattgttat cgttccaata
     4021 tcaccaccgg aatacggcgg gacgtccatg aacgatattg ttcgtaatca gaagtttttt
     4081 gtgccaatgc ctgaaaaagt ttttcacgct cgtccccttc ggcttcgcga gcacgaaccg
     4141 tgaagcgtgc atctttgacc tgtactgttg cggttgagtc tgcttgcagg ttaagccacc
     4201 aagcggggtg attatctttc ccaaggtttg aggcgatgac aacaaaggca tctccatatg
     4261 gcaaatacat caaggctttt gtacggagtt gccccgtttt cttgcctttg gtggttagaa
     4321 gaagcacggg gagtccgcga atggtattgc caatgcgacc tgaactccac agataaagct
     4381 tgaggtgaat ggaccaaaat agcttgataa aagggttatg tttggctgac atactatggg
     4441 gttaagcctg ggcaaccacc ctgatggagc gggtctacgg gtaagccaaa cgcctcgtaa
     4501 acgggcgtac cgtcccattc ggcttgcaag ggcaagccca gggcaaatgc cacggttgga
     4561 gctgtatcga aaatatagac cggcgttttt aactcaccgg ggacaatttg cggtccgcta
     4621 atgatccagg gaatggtcat agcttcgggg atattcgtgc catgtggagg gtcggttaag
     4681 gcaccaccat ggtcagatgt aatgatgaac agcgtgccgt cataatatcc gttctttttc
     4741 atggcactga taaaacgccc aaacgattgg tcgtttttct tgtaagcgct caattgctgt
     4801 ttggaaagcc agccatactc atgcccgacc acgtccccat cggggaagtg caccatcatc
     4861 agcccaaaac ctaggtcaat ctgttcaagc gccatgttat ctaaggtggt cttatcttta
     4921 gcttcatcag tgttatccac ccagccgaag aagtctgtgc tttctgtgat ttggcgtagt
     4981 ttttccttgc caaccacgag cacagtccgc aaaccgacag aacgtgtgag gtcgaagata
     5041 tcggtgccaa gagcataacc attttgagga acatactcat tccaacgaac cgtattttta
     5101 gcaggacagg tgccagtcac caaagcggag tgagcgggta aggtcgtgct gggcatgatg
     5161 gtctgcgcag tgagcgtgta agctccattc ttcatcatct cttggacgtt ggtcatcccg
     5221 gctgcatcaa tggcgtcggg gcgaagtcca tcaaaggtga cgatgaaaac gcgtgagata
     5281 ggttgtggcg cagggatagt aactgtcggc tggggcgttt caggcgcagg cggaattggg
     5341 ctaggcgtca gcgaggcagt agggattggg ggtgtaagag tctcggtcgg aggggtagaa
     5401 gcggaagcag agacacaggc gtgtagaaag atcgagagca gagtcaggat gcggagtttt
     5461 cgaatcatat aaagggatgt tttctatcta ggtcttacag gggtattttt cgtggattct
     5521 gggttggtct tcgccaaaga tcttaaagac gggaatgcca tcccattctt cttggttgat
//
LOCUS       DAOQJE010000106         5551 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_8460_length_5551_cov_7.705968, whole genome shotgun sequence.
ACCESSION   DAOQJE010000106 DAOQJE010000000
VERSION     DAOQJE010000106.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 5551)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5551)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5551
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8460_length_5551_cov_7.705968"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..963
                     /locus_tag="PLT08_04135"
     CDS             <1..963
                     /locus_tag="PLT08_04135"
                     /inference="COORDINATES: protein motif:HMM:NF014647.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HNE03682.1"
                     /translation="GEVTDLGGIDDKSFNANGWAGAQKAAAELGVEAKYLESTQQADY
                     AKNIQQFVDEGTNLIVTVGFLLGVDTATAAKANPDTHFAIVDYAYPDCFGTQVEGKDC
                     GSATELPNVLGLTFQIDQAGFLAGYAAAASTETGKVATFGGINIPPVASFMIGFEKGV
                     LYYNEQNGTAVEVLGWNTEANDGSFTGNFDSLDDGRSFAESFVQEGADIIMPVAGPVG
                     LGSAAYCQESGACKIIGVDVDWTVSAPEYKDIILTSVLKNINVAVYDAIQAEVDGTFA
                     GGVYVGTVENGGVGISDVAGASDDLKAQLDAVTQGIIDGSIATR"
     gene            1154..2686
                     /locus_tag="PLT08_04140"
     CDS             1154..2686
                     /locus_tag="PLT08_04140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNE03683.1"
                     /translation="MTNVLELRGITKRFPGVLANDKISITLREGKILALLGENGAGKS
                     TLMNILYGLYKPDEGQIIVRGKEVDIQGPNDAIAQGIGMVHQHFMLVPVMTVTENVML
                     GIEPTKNGIFLDKEKVANRIREISDEYGLEVDPNAYIKDLPVGIQQRVEIIKVLYRHA
                     DILILDEPTAVLTPQEVEGLFKIIDTLIKAGKSIIFITHKLKEVLAVADDITVLRLGT
                     VVGSIDPKEATSEILATMMVGRDVTLVVQKKLAEPGKPVLDVKDLYVRDERQNMTVQG
                     VSFEVRKGEVLGVAGVQGNGQTELVYALTGLLPLEAGTIHLLDEAIHHTSPRHILEKG
                     VAHIPEDRQRHGLILSFPIHDNMMLCTYYKDPFAKGISLQQKTIFENADQLVKQYDMR
                     TPNIYVNAGTLSGGNQQKVIVAREFSRPIELLIASQPTRGLDVGSIEYIHSQIIKKRD
                     EGSGVLLVSSELDEILALSDRIAVMYKGQIMAIVDASKATKEQLGLLMAGVHPSETSN
                     KN"
     gene            2698..3885
                     /locus_tag="PLT08_04145"
     CDS             2698..3885
                     /locus_tag="PLT08_04145"
                     /inference="COORDINATES: protein motif:HMM:NF014684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNE03684.1"
                     /translation="MTTTATPAPDAGTKETKRNSLWQELLIPILAVVTGLIVGAIVIF
                     VSGDNPFVAYGALFAGSFGTPADYVEGFKVLFSTGDTAALIKAIYPFTESLVTATPYI
                     FAGLSVALGFRCGLFNIGAEGQFFIGALCSAFVGYSLKGLPMIVHLPLAILGGALGGA
                     IWGAIPGYLKARFGAHEVVNTIMMNWIAFRLSDWLLSGPMKSSGFRPVTPNVEASAEL
                     PRFFPEPLRFNLGFFLALLAAYLLYWFLFKTTLGFEIRSVGANPDAAKYAGMNIIRNF
                     VLVMFLAGGLAGLAGTAQVLGVDHWVGQGFSAGYGFDAIALALLGRSHPAGVVLAALL
                     FGFLRSGATDMQSMAGIPIDIISIIQGMVIVFIAAPDIIRWLYRIRATKKDDAVLTRG
                     WGK"
     gene            3897..5159
                     /locus_tag="PLT08_04150"
     CDS             3897..5159
                     /locus_tag="PLT08_04150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNE03685.1"
                     /translation="MKNTKPEISSNTEKRQRISYGLVLLAVGLFIYFVFGLQTEAGTV
                     AKFGLNLAGSQAIEISDLSVPALPVVYFMAFLAVFAGAFQLARGVRSTNWLVGILAVT
                     IVVSFLMWAAQDKSFNLTGMLSSSFVRATPIALAALCGVISERSAVINIGIEGIMLIS
                     AQLAVLTATVTKSLYVGLFAAIIAGGLIAALHAYLVIRFKVDQTVSSVAINIFGAGTT
                     AFISSRFMQRAGDQLNNSGTFPIISIPGLSKIPVLGPVLFENNLIIYLAIILVFVMHI
                     VLYYTPWGLRTRAVGEHPKAADTLGINVYFTRYINVIAGGMIAGLGGAYFTLGSVGRF
                     DEAMTAGKGFIGLAAMIFGNWNPIGAYTSSLIFGFADSLQVKLQILRIPIPSEFLLMA
                     PYIVTMIILTGVVGRAIPPAADGQPYEK"
     gene            5292..>5551
                     /locus_tag="PLT08_04155"
     CDS             5292..>5551
                     /locus_tag="PLT08_04155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE03686.1"
                     /translation="MKNLTRLFHLFVVCALFLGACNLPSNNPQGAAATYAAQTVAALL
                     TPSTTPTTSLTSNNTATPSFTPSPTNTSTPAATATPTCPQAQ"
BASE COUNT         1278 a         1403 c         1337 g         1533 t
ORIGIN      
        1 ggtgaagtga ccgacctcgg cggtattgat gacaagtcct tcaatgccaa tggttgggct
       61 ggcgctcaaa aagctgctgc cgaactcggt gtggaagcca aatatctcga atccacccag
      121 caggctgatt acgccaagaa cattcagcag ttcgttgacg aaggcaccaa cttgatcgtc
      181 accgtcggct tcttgctcgg cgttgatacc gctaccgctg ccaaggcgaa ccctgatact
      241 cacttcgcga tcgtagacta tgcttaccct gactgcttcg gcacccaggt tgaaggcaag
      301 gactgcggtt ccgcgaccga actccccaac gttctcggtt tgaccttcca gatcgaccag
      361 gctggcttcc tcgctggtta cgctgctgcc gcctccaccg agaccggtaa agttgccacc
      421 ttcggtggta tcaacattcc tccggttgct tccttcatga tcggtttcga aaagggtgtt
      481 ctgtactaca acgaacagaa cggcaccgct gtggaagtcc tcggctggaa cactgaagcc
      541 aacgatggtt ccttcaccgg taacttcgac tccctcgatg atggtcgctc cttcgccgaa
      601 tccttcgttc aggaaggcgc tgacatcatc atgcctgtgg ctggacctgt tggtctcggc
      661 tctgctgctt actgccagga atctggcgct tgcaagatca tcggcgtgga tgttgactgg
      721 accgtttctg ctcctgaata caaggacatc atcctcacca gcgttctcaa gaacatcaac
      781 gttgctgtgt acgatgccat tcaggctgaa gttgacggca cctttgctgg tggcgtgtat
      841 gttggtaccg ttgaaaacgg cggcgtcggt atctccgatg ttgctggtgc ttctgacgac
      901 ctcaaggctc aacttgacgc tgtcacccag ggcatcatcg acggctccat cgcaaccaga
      961 taatctggct ctactgcact acaaaagccg gaggaggggt ttccctcccc cggcttttta
     1021 tttttcttat tgctcgtaga tctcaaaggt tttttcaatt tgtcatacat tttccatgca
     1081 ccatccttta taattggggc gtgcaaaaaa aaccttttgg atctatcaaa gtaaagatag
     1141 tggagggtat ggcatgacca atgttttgga actgcgtggc attaccaaac gtttccccgg
     1201 tgttcttgca aacgacaaaa tcagtattac tctgcgtgag ggaaaaattc tcgctttgtt
     1261 aggtgagaac ggtgcaggca aaagtacatt gatgaatatc ctttatgggt tgtacaagcc
     1321 tgatgaagga cagatcattg tccgcggtaa agaggtggat attcaaggac ccaacgacgc
     1381 catcgcacaa gggatcggca tggtgcatca gcactttatg ctcgtccccg tgatgacggt
     1441 caccgagaat gtaatgctcg gtattgaacc cacaaagaac ggtatatttt tagacaaaga
     1501 aaaggtcgcg aaccgcatcc gtgaaatttc ggatgaatac ggtttggaag ttgacccgaa
     1561 tgcctatatc aaagatctgc ctgtgggcat tcagcagcgc gtggagatca ttaaggttct
     1621 ctatcgccat gcagatattc tcatcctaga cgagccgaca gcggtactca ccccgcaaga
     1681 agtggaaggc ttgttcaaga tcattgatac gctcatcaaa gcgggcaaat ccattatttt
     1741 cattacgcac aaattgaaag aagtgttggc agttgcagat gatattaccg ttctgcgcct
     1801 cggaactgtg gttggatcga ttgacccgaa agaagctact tctgagattt tggcaaccat
     1861 gatggtagga cgtgatgtga cattggtggt tcaaaagaaa ttagctgaac ctggcaaacc
     1921 tgttttggat gtcaaagact tgtatgtacg agatgaacgt caaaatatga ccgttcaagg
     1981 cgtatctttc gaagttcgta aaggcgaagt ccttggcgtt gctggtgttc aagggaatgg
     2041 gcaaacggaa ttggtctatg ccctgaccgg gttattacct ctggaagcag gtacgatcca
     2101 tttgttggat gaagccattc atcacacgtc tccacgtcac atccttgaaa aaggtgtggc
     2161 tcacattcca gaagatcgcc agcgccatgg cttgatcctt tccttcccca tccacgacaa
     2221 tatgatgtta tgcacctact acaaggaccc atttgcaaag ggcatttcct tgcagcagaa
     2281 aaccattttt gaaaacgcag accaattggt aaagcaatat gatatgcgca ccccgaacat
     2341 ctatgtcaat gcagggacgc tttccggtgg taatcagcaa aaagtgatcg tggcgcgtga
     2401 attttcacgt cccattgaat tattgatcgc ctcacaaccg acccgtgggt tggacgttgg
     2461 ctcgattgaa tacatccact cacagattat caaaaagcgc gacgaaggtt ctggcgttct
     2521 gctcgtttca tccgaattgg atgaaattct cgctctctca gaccgtatcg ctgtgatgta
     2581 caaaggtcag atcatggcga tcgttgatgc aagcaaagcg accaaagaac aacttggtct
     2641 gctcatggcg ggtgttcatc cctccgaaac ctcgaacaag aattaggagt tgtatctgtg
     2701 actactacag ctactcccgc cccagatgct gggacaaaag aaacgaaacg taattcgctt
     2761 tggcaggaat tgttaatccc tattcttgct gttgtgaccg ggttgatcgt tggcgccatt
     2821 gttatcttcg tcagcggcga caatcccttt gttgcatacg gtgctctctt tgccgggtct
     2881 ttcggtaccc ctgccgatta tgttgaaggc ttcaaggttt tattctccac aggagataca
     2941 gccgcattaa tcaaagccat ttacccattt actgaaagtc ttgtaacggc tacgccctat
     3001 atttttgcag ggctttcggt ggcactcggc tttcgttgtg gtttgttcaa tatcggtgct
     3061 gaaggtcagt tcttcattgg tgctctttgc tctgcctttg ttggctacag cctcaaggga
     3121 cttcccatga tcgttcactt gccgcttgct attttaggcg gcgccttggg cggagcaatt
     3181 tggggagcca ttccgggcta tctcaaggct cgatttggcg cccatgaagt ggtcaacacc
     3241 atcatgatga attggatcgc tttccgattg agcgactggc tcttaagcgg accgatgaag
     3301 tcttctggtt ttcgcccggt gacccccaat gtggaagcct ctgcagaatt gccacgcttc
     3361 tttcccgaac cgttgcggtt caacctcgga ttcttccttg ccttgctggc agcgtatctc
     3421 ctctactggt tcttgttcaa gaccacgcta gggtttgaga tccgctcggt gggcgcaaac
     3481 ccagatgccg cgaaatatgc aggcatgaac atcatccgta actttgtatt ggtcatgttc
     3541 ctagcgggcg gcttggcggg cttggctgga actgctcaag ttctcggcgt ggaccactgg
     3601 gtgggtcagg gcttttcagc cggatacggg tttgatgcga ttgcgttggc tttgctgggt
     3661 cgcagtcatc cggcaggagt cgtccttgcg gcgctgctat ttggtttcct ccgtagtggt
     3721 gccaccgata tgcaatctat ggctggcatc cccatcgaca ttatctcgat cattcaaggt
     3781 atggtgattg tgtttatcgc cgcacctgat atcatccgct ggctttaccg catccgcgcc
     3841 acgaaaaagg atgatgctgt tcttacccgt ggttggggta aataaacgag gcttgcatga
     3901 aaaacaccaa acctgaaatt agctctaata ctgagaaacg ccagcgcatt tcatatgggc
     3961 ttgtattgct tgcggtgggt ctgttcattt actttgtctt tggtttacag acagaagctg
     4021 gcacagttgc caaattcggt ttaaatttag caggctcaca agccattgaa atttcagacc
     4081 ttagcgttcc ggcattgcct gttgtgtatt tcatggcatt tctcgctgtt tttgccggtg
     4141 cctttcaatt ggcacgtggg gtgcgctcta caaactggtt ggttggaatt cttgcagtta
     4201 ccattgtcgt ttcctttttg atgtgggcag cgcaagataa atcctttaac ctgaccggta
     4261 tgttgagtag ttcattcgtc cgtgctacac cgattgcttt agccgcctta tgtggtgtga
     4321 tcagcgaacg ctcagcggtt atcaacattg gtatcgaagg cattatgctc atctcagcac
     4381 agcttgctgt tctcactgct acagtgacga aaagcctgta tgttgggctc tttgctgcca
     4441 ttattgcagg tggtttaatc gctgccttgc atgcctattt ggtcattcgc ttcaaggtag
     4501 accaaactgt cagtagtgtt gccatcaaca tcttcggtgc cggtacaact gcttttatca
     4561 gctcacgctt catgcaaaga gcgggtgatc aattaaacaa ttctggcacc ttccccatca
     4621 tctccattcc tgggctttct aaaatccctg tgttgggacc tgttctcttc gaaaacaatc
     4681 tgatcattta tttggcgatc attttagttt tcgtgatgca catcgtgttg tactacaccc
     4741 cttggggctt gcgcactcgc gcggtaggcg aacaccccaa agccgcagac accttgggta
     4801 tcaatgtgta cttcacccgc tatatcaacg tcattgctgg cggtatgatc gctgggttgg
     4861 gtggcgccta cttcaccctc ggttccgtag gtcgttttga cgaagccatg actgcgggta
     4921 aaggtttcat cgggctcgcc gctatgatct tcggaaattg gaatcccatc ggtgcgtaca
     4981 cttcatcatt aatctttggt ttcgcagact cgttacaggt caaactacaa atcctgcgca
     5041 ttccgatccc ctcagaattc ttgctcatgg cgccatacat tgtgaccatg atcatcctga
     5101 ccggtgttgt aggacgtgcc atcccacccg cagcggatgg tcaaccgtac gaaaaataac
     5161 gccttattcg tacgccatac ttgcctcaaa aaacaggagt tttcaccact cctgtttttt
     5221 attttgggaa ataaggtgga tagcctaaaa tttatcttgg ggtaaaattt tgataattaa
     5281 gaggagaagc aatgaaaaac ctaacacgcc tattccatct gttcgtcgta tgcgctttat
     5341 tcttgggtgc atgcaacctg ccctcgaata atcctcaggg tgcagcggca acctatgctg
     5401 ctcaaaccgt cgcagcttta ttgaccccat ccacaacacc aactacatct cttacatcaa
     5461 ataacacagc tacaccaagc ttcacaccat ctccgacaaa tacgagcacc cctgccgcca
     5521 cagcaactcc aacctgcccg caggctcaat t
//
LOCUS       DAOQJE010000107         5544 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_8478_length_5544_cov_6.054108, whole genome shotgun sequence.
ACCESSION   DAOQJE010000107 DAOQJE010000000
VERSION     DAOQJE010000107.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 5544)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5544)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5544
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8478_length_5544_cov_6.054108"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..492
                     /locus_tag="PLT08_04160"
     CDS             <1..492
                     /locus_tag="PLT08_04160"
                     /EC_number="1.1.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF012678.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HNE03687.1"
                     /translation="HAAKSLRRVYQNGADKEAREGMALASLFGGLALANAALGAVHGF
                     AGPLGGMLHAPHGAICARLLPLVMEANIEAMKAEKHAGLARYEEVARTLTGNDAATVE
                     DGLQWVKELVRELNIPALSAHGMTKAHIPEAVQKTLNASSYKGNPILLSPEILQKILE
                     KAL"
     gene            700..2358
                     /locus_tag="PLT08_04165"
     CDS             700..2358
                     /locus_tag="PLT08_04165"
                     /inference="COORDINATES: protein motif:HMM:NF012706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HNE03688.1"
                     /translation="MKSNLLKGLSAIVLIGLLLTACGGGAPAATEAPAGEATSAPAEA
                     TEAPAVSGGTLTLGIPVEPETLDPGDAVYVQEQFILMNLFDSLLSISPDGELHPGLAT
                     TWEPNAEYSEFTFTLREDVTFHDGTPFNAEAVKASFDHINSDAVLESGGKSLLTDHSY
                     VETVVVDDYTVTVKFGASYPLFLRDAARQWLSISSPAALEQYGADYGRNPVGTGPFKF
                     VQWDAQSQIVIERNPDYNWGPEFASHQGPALLDQVVFRILPEAATRLTAFETGEIQIA
                     GEPPALDAASLVDAGTASLENFAQPGIPAILMINSAKAPTDNVNVRKAMILAVNQEEL
                     AQTAFQELGLPAYNVVSPTTWAYDEGAANLYRYNPEEAARLLEEAGWVDSDGDGVREK
                     DGETLTIDWPDNPAWSEAFNELIIGYLSDVGFNVQYRSMDDGAAYEELLAGNYTLVYM
                     YWTRPDPSPLRYLFHSENNNGGGAWSNFTYADLDNALADADTNTDEAARKQDYVTAQN
                     IIMENALAIPMFTVNTSYLTAPSVQGFTFDLEGYPFLYDMSVSQ"
     gene            2471..3397
                     /locus_tag="PLT08_04170"
     CDS             2471..3397
                     /locus_tag="PLT08_04170"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNE03689.1"
                     /translation="MLNYLIRRLFTSVLVLFGISVLVFSVIHLVPGDVTMAILGRQKV
                     NEEKVAELREQLGLNDPLYVQYGRYVSGALRGDLGKSIRTNQSVSASISEQLPGTFSL
                     AMSALAVALSIGAIFGLLAALRHGTWVDTIVMGLSVSGLSIPTFWMGLLLILFFSVKL
                     KLFPSISNGSSFADLFLPALTLGLPEAAVVARMVRASMLDVLNKEYITTARAKGLFER
                     SVIIKHALRNALIPVVTFVGLQMAYLLGGATIVETMFARQGIGRLAVQAIYSRDYPMV
                     QGVVLITATIYVLINTITDITYVLLNPKIRLK"
     gene            3394..4281
                     /locus_tag="PLT08_04175"
     CDS             3394..4281
                     /locus_tag="PLT08_04175"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNE03690.1"
                     /translation="MTANLSSTPIKSNSPGVRLFRQILRNRSAQFGGILLILLIVASY
                     AAPFITAKDPNKNAPSVALEAPSFEYPMGTDNLGRDNFTRFLYGGQLSLRVGLTGIII
                     GALIGILLGLISGYFGGWLDSFISWFNDVLMAFPDILLALAIIAILGPGINNAMLAIG
                     ISFIPSFMRLTRSSVFALREMTYIEAAKALGSSDTRIMLKHILPNSIRTLLVLITLGI
                     GSSILAGAALNFLGLGAEPPTAEWGAMLNAGMKYVRQAWWLTFFPGLGIFLAVLSINL
                     IGDAVSDAVAGTTIIKESK"
     gene            4283..4969
                     /locus_tag="PLT08_04180"
     CDS             4283..4969
                     /locus_tag="PLT08_04180"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HNE03691.1"
                     /translation="MASLSGKTAIITGGSTEIASAVALKLAEAGANICLCGRQADLLA
                     MAVDRVKQAGGKGFFVESNLKSLEEAEGVVHQTTAEFGGLDILILISPFWSGGMIHSH
                     SVKTWDLVMDANLREPFLMSRAALPFLREQKHGEVMTIGSDSALGVYPQDGAFAVAMH
                     GLTTLMELIRAENSEHGIRTHILSPGVAVSAPFDVEGKPNLMPDHVADWVLWLLTRPD
                     HLRGNSPILI"
     gene            complement(5015..>5544)
                     /locus_tag="PLT08_04185"
     CDS             complement(5015..>5544)
                     /locus_tag="PLT08_04185"
                     /inference="COORDINATES: protein motif:HMM:NF012892.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="cation-translocating P-type ATPase C-terminal
                     domain-containing protein"
                     /protein_id="HNE03692.1"
                     /translation="GKPLPLLPLQLLWLNLLTDGLLGLGMGVENPEKDTMKRKPYSPT
                     EGVFSRGAGVQTIWVGALIGALALGLGSWYYFTDRPEWQTMIFTSLAFMQIFQALASR
                     SDKDSLVKIGVMSNPVLAGMALLVFVLQMAVIYIPALANFFEVIPLRWHDLSIAAGTG
                     LLVFLVMEVSKWFKK"
BASE COUNT         1273 a         1411 c         1416 g         1444 t
ORIGIN      
        1 catgcggcaa aatctttacg ccgtgtgtat cagaatggtg cggataaaga agcacgtgaa
       61 ggcatggcat tggcgagtct gtttggcggg ttggcattgg cgaatgccgc acttggtgcc
      121 gtgcatgggt ttgcaggtcc gctgggtggg atgttacacg ccccgcatgg agccatttgt
      181 gcgagactgt tgcctttggt gatggaagcg aatatcgaag cgatgaaggc agagaagcat
      241 gccgggttgg cgcgctatga agaagtggca cgtaccttaa caggaaatga tgccgctact
      301 gtagaggatg gtttgcaatg ggtgaaggag ttggtgcgcg agctgaatat cccagccctt
      361 tctgcgcatg gtatgactaa ggcgcatatc cctgaagccg tacagaagac cttgaatgcc
      421 agtagctaca aggggaatcc aattctgtta agccctgaga tattgcaaaa aattttagag
      481 aaagcccttt aacatcagtg atatttctct tttgggtggg gtaaatattc aggtttttac
      541 ctcttctagg taaaaacctt ttcgttttgg tacgaatagg aatttaaaca ttttatgagg
      601 tgtttttagg ttaagtgtaa aatgatataa ttcctatagg attagtttta taaagtattg
      661 gctttataat accggtcgca atttataaaa ggagataata tgaaatccaa cttacttaag
      721 gggttgtccg ctatcgtatt gatcggactg ttattgaccg catgtggtgg tggggcgcct
      781 gcggctacgg aagcacctgc cggagaagcc acatccgcgc ctgcagaagc gaccgaagcg
      841 ccagccgttt ctggtggaac gctcacgctt ggcattccgg ttgagccgga aacgctcgac
      901 cctggagatg ccgtctatgt gcaggaacag ttcattctga tgaatctgtt cgactccctt
      961 ctttccattt cgccggatgg agagttgcac ccgggtcttg ctactacctg ggaacccaat
     1021 gcggagtatt ctgaattcac cttcacgttg cgcgaagatg tgaccttcca tgatggcaca
     1081 cccttcaatg ccgaagcggt gaaggcttca ttcgatcaca tcaacagtga tgcggtgttg
     1141 gaatctggcg gtaagtcttt gttgactgac cactcttacg tggagacagt cgttgtcgat
     1201 gattacaccg tcaccgttaa gttcggcgct tcgtatccgc tcttcttgcg cgatgccgcc
     1261 cgtcagtggt tgagcatttc ttcgcctgcc gccttggaac aatatggcgc tgactatgga
     1321 cgcaacccgg tgggaactgg tccgttcaag tttgtgcagt gggatgccca gtctcagatc
     1381 gtgatcgaac gcaaccccga ttacaactgg ggacctgaat tcgcgtccca tcagggaccg
     1441 gctctgctcg atcaggttgt cttccgcatt ttgccggaag ccgccacccg tttgaccgct
     1501 tttgaaaccg gtgagatcca gatcgctggt gaaccgccag ccttagacgc cgcctctttg
     1561 gtcgacgctg gaaccgccag ccttgaaaat ttcgcccagc ctggcatccc tgcgattttg
     1621 atgatcaact ctgccaaggc tccaaccgat aacgtcaacg tccgtaaggc gatgattctt
     1681 gccgtcaatc aggaagagct tgcgcaaacc gccttccaag aacttggctt gcctgcctac
     1741 aacgtcgtct ccccgaccac ttgggcatat gacgaaggcg ctgcaaacct gtatcgctac
     1801 aatccagaag aagcggctcg tctcctcgaa gaagcgggct gggttgactc tgatggcgat
     1861 ggtgttcgcg aaaaagacgg cgagacattg accattgatt ggcccgataa cccagcttgg
     1921 tctgaagcct tcaacgaact cattatcggt tatctctctg acgttggctt caatgtccaa
     1981 taccgctcca tggatgatgg tgccgcctac gaagaattgc tcgctggcaa ctacaccctg
     2041 gtctacatgt actggacccg ccccgatcct tctccattgc gctacttgtt ccactctgag
     2101 aacaacaatg gcggcggggc ttggtcgaac tttacctatg ccgacctcga taacgcgctt
     2161 gccgatgccg ataccaacac cgacgaagcc gctcgcaagc aggattacgt cacggctcag
     2221 aacatcatca tggaaaatgc acttgccatt ccgatgttca cagttaatac ttcctacctc
     2281 accgcgccat cggttcaggg tttcactttc gacctcgaag gttatccttt cctgtacgac
     2341 atgtccgttt cacaataaat ccaaaccaac ctttgctctt tcaacgagca tgttcggcaa
     2401 ataagtaata caatgggtgt gccgcatctc tttatagggg tgcggcacac ttaaaaactc
     2461 caggctttcc atgctcaatt atttgatacg gcgtcttttt acatcggtct tggtactctt
     2521 tggcatttcg gtgttggtct tttcggtcat tcacctcgtg ccgggcgatg tcaccatggc
     2581 gatccttgga cgccaaaaag tgaatgaaga aaaagtggca gaactgcgcg agcaactcgg
     2641 gctcaatgac ccgctttacg tgcagtatgg acgttacgtc tctggggcat tgcgcggaga
     2701 cttggggaaa tcgatccgca cgaatcaatc cgtttctgca tccatcagcg aacaactgcc
     2761 cggcacattc tctttggcaa tgtctgcatt ggcggtggca ctcagcattg gcgcgatctt
     2821 tggtctgttg gcggcattac gtcatggtac atgggtcgat acgattgtga tgggtctatc
     2881 tgttagtgga ctttccatac ccactttttg gatgggtctg ctcctcatcc ttttcttctc
     2941 tgtgaagtta aaactcttcc caagcatttc caacggctct tccttcgccg atctctttct
     3001 ccccgccctg acgttgggac tccccgaagc cgcagtcgtc gcgcgcatgg tacgtgccag
     3061 catgctcgat gtgctcaata aagaatacat cactaccgcc cgcgccaagg gactgttcga
     3121 acgttccgtg attatcaaac atgcattacg aaatgcatta attcctgttg tgacctttgt
     3181 cggtctgcag atggcgtatt tgcttggcgg cgcaaccatt gttgaaacca tgtttgcccg
     3241 tcagggaatt gggcgtttag ccgtgcaagc catctacagc cgtgattacc ccatggtgca
     3301 aggcgtggtg ctgattacag ccacaatcta tgtgcttatc aacaccatta cagacatcac
     3361 ctatgtgtta ctaaacccga agattcgcct caaatgaccg caaacttgtc atccactccc
     3421 atcaaatcca attcgccggg cgttcgtttg ttcaggcaga ttcttcgtaa tcgtagcgcc
     3481 caatttggcg gcatcctatt gattcttttg atcgtcgcct cgtatgccgc accttttatc
     3541 acagcgaaag accccaataa aaacgcccca tctgtagcct tagaagcgcc tagctttgaa
     3601 taccccatgg gcacagataa ccttgggcgt gataacttca cccgcttcct ttatggcggt
     3661 caactgtcat tgcgagttgg gctgaccggt atcatcatcg gcgcattgat tgggattctg
     3721 ctcgggttga tctctggcta cttcggtggc tggctggatt cgttcatctc ttggttcaat
     3781 gatgtgctga tggcgttccc tgatattttg ctggcattag ccatcatcgc cattctcgga
     3841 cccggtatca acaacgctat gttggcgatt ggtatctcgt tcatcccttc gtttatgcgt
     3901 cttactcgtt caagtgtgtt tgccttgcgt gaaatgactt acatcgaagc cgcgaaagca
     3961 ctcggcagtt cagacacgcg aatcatgctc aagcatattt tgccgaactc gattcgtact
     4021 ttgttggtgc tcatcacact tgggataggc tcatctatcc ttgcgggtgc ggcgttgaac
     4081 tttcttgggc tcggtgcaga gccgcccaca gcagaatggg gcgctatgct caatgccggc
     4141 atgaaatacg tccgccaggc gtggtggttg accttcttcc cggggttggg aattttcctc
     4201 gcggtgcttt cgatcaacct gattggtgat gcggtcagtg atgcggtagc agggacgacc
     4261 atcatcaagg aaagcaaata gcatggcatc tttatcgggc aagaccgcca tcattacggg
     4321 tggctccaca gaaatagcaa gtgctgttgc attgaagcta gcagaagcag gggcgaatat
     4381 ctgtttatgt ggcagacagg cagatttatt agcgatggca gtagacagag tcaagcaagc
     4441 gggaggtaaa ggcttctttg tagaatccaa cctcaagtcg ttggaagagg cggaaggggt
     4501 tgtccatcaa accacggctg aatttggcgg tttggatatc ctgatcctta tctcgccctt
     4561 ttggagcggt ggcatgattc acagtcacag cgtcaagacc tgggatctgg tgatggatgc
     4621 caatttacgt gagccgtttt tgatgtcgcg tgcggcgctc ccttttttgc gagaacaaaa
     4681 acatggtgag gtgatgacaa tcggctcaga ctctgcgttg ggcgtatacc cacaagatgg
     4741 ggcatttgcc gttgccatgc acggactgac aactttgatg gagctcattc gcgctgagaa
     4801 tagcgagcat ggcattcgta cacatattct ttcgccgggc gtggcggtat ctgcgccttt
     4861 tgatgtggaa ggaaaaccaa acctcatgcc agaccatgtt gctgattggg tcttatggtt
     4921 gcttacccgc ccagaccatt tacgcggaaa cagccctatt cttatctaac aaaaaagacc
     4981 tgcttcaaaa gcaggtcttt tattttctaa caagttattt cttaaaccat ttgctgactt
     5041 ccataacgag gaataccagc aagcctgttc ctgcggcgat agatagatca tgccagcgta
     5101 gaggaatgac ttcaaagaag tttgccaatg cagggatgta gatgaccgcc atctgaagaa
     5161 caaagaccaa cagcgccatg cccgccagaa cggggttact catcacgccg atcttgacga
     5221 gcgagtcttt gtctgagcga gacgccagcg cttggaagat ctgcatgaat gccagagagg
     5281 tgaagatcat cgtctgccat tcggggcggt cagtaaagta ataccaggag ccaagcccaa
     5341 gggcaagtgc gccgattaag gcgccaaccc aaatcgtttg tacaccagcc ccacgcgaga
     5401 agacgccctc agtcggggag taaggtttgc gcttcatcgt atccttttca ggattttcca
     5461 cgcccatccc taaaccgagc aaaccatcag ttagcaggtt gagccacagt aactgcaaag
     5521 gcaagagcgg caatggctta ccaa
//
LOCUS       DAOQJE010000108         5536 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_8498_length_5536_cov_4.635468, whole genome shotgun sequence.
ACCESSION   DAOQJE010000108 DAOQJE010000000
VERSION     DAOQJE010000108.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 5536)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5536)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5536
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8498_length_5536_cov_4.635468"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..687
                     /locus_tag="PLT08_04190"
     CDS             <1..687
                     /locus_tag="PLT08_04190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE03693.1"
                     /translation="MTMTIGAWCLGNAWLAFFTARRWNWQLVYPALSYLWSFGLLEGM
                     IVVSFREKLNLDYPVAWFYVSVISLNAIAAAFGIVDWLRVRPAGSPSEPMTGLMRGVA
                     TAFVLFVGFLGVYGLSVQAGAPATTGEIFPEAMTTLTLRSFGAFFLALTIGMLPLLFE
                     KNRAPFLNYSFLAFGLVIIITIAAFAYFPLFNFGEHPFGSLYFLAYFVAAGVSIFFFR
                     KFGTGATTKA"
     gene            complement(688..1470)
                     /locus_tag="PLT08_04195"
     CDS             complement(688..1470)
                     /locus_tag="PLT08_04195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE03694.1"
                     /translation="MPPSTIGGINLLSEPEKRAIYTKYIPQALLDKYNLPPLTSAAGY
                     NLLQFRFANGSTDVEMRLYHKVDFPDPILYAHLTDTMNAQIHVLLYVLNDPDSPRYDV
                     DKMPDGTPTKFGTLRRNIEAETKACEAGLAPGQVRRGLRLLGGAIEAFEGFVTALGHD
                     MYFVEPLYYHNAVIFERYGFSYQMGKRLMESINTGFQPGGDLHSQLDGSNAFRKTEAA
                     ESIRKRSWAIHDGILGEPFTNVTMYKRVGHSAGVDTTKGCKW"
     gene            complement(1529..1990)
                     /gene="smpB"
                     /locus_tag="PLT08_04200"
     CDS             complement(1529..1990)
                     /gene="smpB"
                     /locus_tag="PLT08_04200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HNE03695.1"
                     /translation="MTDNIKIIANNRKAGFEYTLIETFEAGISLQGSEIKSIRAGQIS
                     IQEAYVDVENGQEAWLLEAHIAPYEQAGKYFNHEPKRKRRLLMHKKEIRELWNNVRIK
                     GMTIVPTKVYLKDGRAKVEIALAKGKKAYDKRATIAKRDEARDKERAMRVR"
     gene            complement(2063..3316)
                     /locus_tag="PLT08_04205"
     CDS             complement(2063..3316)
                     /locus_tag="PLT08_04205"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNE03696.1"
                     /translation="MRFNLFKQSDIPEKYRSNFLHLYFDIAWFGVLSGTAVNFLNVYA
                     TRLGASASQVGLLTAASAVVNLFLAIPAGHWISKRHTGMAVFWSSVLFRIGYFMWIPL
                     PWFLSNQGQIWALIILTFLMAIPLTPLGVGFNALFAEAVPDRYRARVAGTRNVTFAIA
                     YMLTSLVAGYILKNTEFPGGYQVIFVIGAFGAAMSSYHIYHVKPMQDDTPAAPSAPVP
                     ASAPKPDSPRGMTSALRLDIWKSPFKNVLLALFIFHFAHYLSNPLYPIFNVRVLNLND
                     SHLGNGTALYYLSVLLASTQLGRIVHRFGNKRITGWGVAGMASYPLMLAFAHTVWQFY
                     VLSFLGGFLFALVNGSYANYMLERIPADDRPSHLAWYTLMFNFAILTSSLIGPAVAEV
                     IDLVPAMIAFGIARILAGLFLLKWG"
     gene            complement(3383..>3687)
                     /locus_tag="PLT08_04210"
     CDS             complement(3383..>3687)
                     /locus_tag="PLT08_04210"
                     /inference="COORDINATES: protein motif:HMM:NF012675.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glutaredoxin domain-containing protein"
                     /protein_id="HNE03697.1"
                     /translation="ATVAEVHSFQFSSPEEFMSQPNITVYGADWCGDCRRSKRFLTEK
                     NIPFTWVDVDQDQAGEALVLEKNNGKRIIPTILFEDGTFLAEPSDEALADKLGLPV"
     assembly_gap    3688..3787
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<3788..4959)
                     /locus_tag="PLT08_04215"
     CDS             complement(<3788..4959)
                     /locus_tag="PLT08_04215"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HNE03698.1"
                     /translation="MTEPKITLYGAYWCPDCRRSKKFLGEQFVPFKWIDIEQDKEAEA
                     LVLQKNNGKRIIPTIVFEDGSFLVEPTNAELAKKLGLKTEAKKTYYDLIIIGGGPAGL
                     TAAIYASREGADVLLIERSGLGGQAGITVGLDNFPGFPEGISGQEFSDRVTQQAKRFG
                     VEILQAVDVERLEEENGYHEVYTSDGKHYHSRSVLLATGASYRRLEVPGEDDYIGAGI
                     HFCATCDGPFYKGAKEIVVIGGGNAAVEEGLHLTNFAEKVTLLARGDKLTASQIAIDK
                     VTEPGSKVDVRYNVSVEGFDGQDSKLKSIKTRNKVSGQVEELHPAAAFVFIGQQPNTE
                     FVKSYVQTDPSGFILTGHDLGHDQDALPFETSVRGIFAAGDARHGSVKQVASAVGEG"
     gene            5129..>5536
                     /locus_tag="PLT08_04220"
     CDS             5129..>5536
                     /locus_tag="PLT08_04220"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF025054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNE03699.1"
                     /translation="MNANQNVAGQDNIFIAIASDGDQIKDITARAGVFSEEEVACVAE
                     IWDDYIENGVESCGYNFIVYREGTQVLGFASYGYRDLTDGVYDLFWIAVDANTRRGGV
                     GRKLLQATEEEVRKLGGRMVIAETSGKALYESTR"
BASE COUNT         1267 a         1356 c         1424 g         1389 t
ORIGIN      
        1 atgaccatga ccatcggcgc ctggtgcttg ggcaatgcct ggctggcgtt cttcacggct
       61 cgccgctgga actggcaact ggtctaccca gccttgtctt atctctggtc ctttggcttg
      121 ttggaaggca tgatcgttgt gtctttccgt gagaagctga atttagatta ccccgtggct
      181 tggttctatg tgtctgtgat cagcctcaac gccatcgccg ccgccttcgg catcgtagac
      241 tggttgcgcg tccgcccggc gggcagcccc tccgagccaa tgaccggtct catgcgcggc
      301 gttgcaacag cattcgtcct ttttgtaggc tttctcggcg tgtatgggct gagcgttcaa
      361 gcgggtgcgc ctgccacaac tggcgaaatc ttccccgaag ctatgaccac cctcacattg
      421 cgcagctttg gcgctttctt ccttgccctc accataggca tgttgccctt gttgttcgaa
      481 aagaaccgcg ccccgttttt aaactacagt tttctggcgt ttggcttggt cattattatt
      541 accattgcgg cgttcgcata tttcccgctc tttaactttg gcgaacaccc ctttggtagc
      601 ctttattttc tggcgtactt tgtggcagcc ggagtttcta ttttcttctt ccgcaaattt
      661 ggtacaggcg ctactacaaa agcatagtta ccacttacaa cctttggttg tgtccacacc
      721 cgcactgtga cctacacgtt tgtacatggt cacattggta aaaggctcgc cgagaatgcc
      781 gtcgtgaatc gcccaagagc gtttgcgaat gctctcggcg gcttctgttt tccggaaggc
      841 gttcgagccg tctagttgag aatgaaggtc gccaccgggt tgaaaaccgg tgttgatgct
      901 ttccatcaag cgtttgccca tctgatacga aaaaccataa cgctcgaaga taactgcatt
      961 gtgataatac aacggctcga cgaagtacat gtcgtgacct agggctgtga cgaagccctc
     1021 gaacgcttca attgcgccgc ccaacaacct cagcccgcgt cgtacctgac ccggtgccag
     1081 cccagcctca caagcctttg tctccgcctc gatattgcgc ctcaacgtgc cgaacttggt
     1141 gggtgtgcca tcgggcatct tgtccacgtc atagcgcgga gagtctgggt cattgagcac
     1201 atacaacaac acatgaattt gagcgttcat ggtgtcggtc aagtgcgcgt ataagatcgg
     1261 gtcggggaag tctactttgt gatacaggcg catttccaca tcggttgagc cgttcgcaaa
     1321 gcggaattgc agcaggttgt atcctgccgc ggaggtcaac ggcggcaggt tgtatttgtc
     1381 taatagcgct tgcggaatgt atttggtgta gatcgcccgc ttttcgggtt cagataataa
     1441 attgattccg ccaatggttg agggaggcat agcttcttct ttcttctttc acctctcctc
     1501 cctatataaa gaggaaagat gaaagaggtc atcttacccg catggcacgt tctttatcac
     1561 gcgcttcatc tcgtttagca atggtggcgc gtttgtcgta ggctttcttg ccttttgcca
     1621 gtgcgatctc gaccttggca cgcccgtctt tgagatagac cttggtcggg acaatggtca
     1681 tgcctttgat gcggacgttg ttccaaagct cgcggatctc ttttttgtgc ataagtaaac
     1741 ggcgtttgcg cttcggttcg tggttgaagt atttacccgc ttgttcataa ggcgcgatgt
     1801 gcgcctcgag caaccaggct tcctgcccat tttccacgtc cacgtaggct tcttgaatgc
     1861 tgatctgccc ggcgcgaatg gatttgatct cagacccttg cagggaaatg cccgcttcga
     1921 aggtttcgat gagagtgtat tcaaaaccgg ctttacggtt gttggcgatg attttgatgt
     1981 tatctgtcac aagatgattt tagcatggaa aattgttgga gaataagcca cagaacccgc
     2041 agagattttg agggctagtt gattagcccc atttgagcag gaacaaccca gcgaggatgc
     2101 gggcaatgcc aaaggcgatc atggcaggga caaggtcaat gacctctgcc acagccggac
     2161 cgatcaacga gctggtgagg atggcaaagt tgaacatgag ggtataccac gccaaatgcg
     2221 aagggcggtc atcggcgggg atgcgctcaa gcatgtagtt ggcatatgaa ccattgacca
     2281 aggcaaacag aaagccgccg aggaatgaaa ggacgtaaaa ctgccaaacg gtgtgcgcga
     2341 atgccaacat cagtgggtac gaagccatac ccgccacgcc ccagcctgtg atgcgtttgt
     2401 tcccaaagcg atggacgatg cgccctaatt gagtggatgc cagcaataca gatagatagt
     2461 agagcgcggt cccattgccg aggtggctgt cattgaggtt gagcacacgc acgttgaaaa
     2521 tcggatacag cggatttgac aggtagtgtg caaagtggaa gatgaacaat gccagcaaga
     2581 cattcttgaa tggtgatttc caaatgtcca ggcgcagggc ggaggtcatg ccgcggggag
     2641 agtctggttt gggggcagag gcaggcacgg gcgcggaagg agccgcaggc gtatcgtctt
     2701 gcatcggttt gacgtggtag atgtgataac tgctcatcgc cgcgccaaag gctccgatca
     2761 caaagatgac ttgataccca cctgggaatt cggtgttctt gagaatgtaa ccagcaacga
     2821 gggaggtgag catgtaggcg atggcgaagg tcacattgcg agtgcctgcc acgcgggcgc
     2881 gataacggtc tggcacggct tcggcaaaga gtgcgttaaa tcccacgcct aatggcgtaa
     2941 gtggaattgc catgagaaag gtcaaaatga tgagcgccca aatttgccct tggttgctaa
     3001 gaaaccaagg caggggaatc cacatgaaat agccaatgcg aaagagcacc gaagaccaaa
     3061 agaccgccat accggtgtga cgtttgctga tccaatgccc agcaggaata gcaaggaaaa
     3121 gattgacgac ggcagaggct gctgtaagca agccgacttg cgaagcgctc gcccccaagc
     3181 gcgtggcata cacattgaga aagttaacgg cggtgccgct tagcacgccg aaccaggcaa
     3241 tatcgaaata gagatgaaga aagtttgatc tgtatttttc aggaatatct gattgtttaa
     3301 ataaattgaa acgcatacat gaaattatac tcacgtatgc gtttcgagat tttgcagctt
     3361 atttgatttt caggttgttt tgttagacgg gtagaccaag tttatctgct agcgcttcgt
     3421 cagatggttc agcaagaaag gtgccatctt caaaaaggat ggttggaatg atgcgtttgc
     3481 cgttgttctt ttccaaaaca agcgcttcac ctgcttggtc ttggtcgaca tcgacccatg
     3541 tgaaggggat atttttttcg gtcaaaaagc gtttagagcg ccgacagtcg ccgcaccagt
     3601 ctgcgccata gacggtgata ttgggttgtg acatgaattc ctccggtgat gaaaactgaa
     3661 atgagtgtac ttccgctacc gtcgcgcnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3721 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3781 nnnnnnncct tcgcccactg ccgacgccac ttgtttaaca ctgccatgcc gcgcatcgcc
     3841 tgccgcaaag atgccacgaa cactggtttc aaatggcaaa gcatcttgat cgtgccccaa
     3901 gtcgtgaccc gtcagaataa atccgcttgg gtcggtctgc acatagcttt tcacaaactc
     3961 agtatttggt tgttgaccga taaacacaaa cgctgccgct ggatgcaact cttccacctg
     4021 accggacacc ttattacggg tcttaatgga ctttagtttt gaatcctgtc catcaaaccc
     4081 ttctacgctg acgttgtatc gcacatccac ttttgagccg ggttcggtca ctttatcaat
     4141 cgcgatctgg ctggcagtca gtttatctcc gcgtgccaag agggtcactt tctcggcaaa
     4201 gttggtcaga tgcagacctt cttccacggc ggcatttcca ccaccgataa ccacgatttc
     4261 ttttgcccct ttataaaacg gaccatcaca cgtggcacag aaatgaatgc ccgccccaat
     4321 ataatcatcc tcaccgggca cttccagtct gcgatatgat gcgcccgtag ctaacaaaac
     4381 agaacgtgaa tgatagtgct tgccgtctga tgtatatact tcgtgatagc cattttcttc
     4441 ttccagccgt tccacatcta cggcttgcag aatttccacg ccgaagcgtt tggcttgttg
     4501 cgtgacacga tctgagaatt cttgtccgct aatgccttca ggaaagccgg gaaagttatc
     4561 taacccaacc gtgatgcccg cttgcccgcc caaaccagag cgttcgatca gcaacacatc
     4621 tgcaccttca cgtgaggcgt agatcgcggc ggtcaacccg gcaggtccgc caccgatgat
     4681 gatcaggtcg taataggttt tcttcgcttc ggttttcaat cccagctttt tggcaagctc
     4741 ggcgttggtc ggctcgacta gaaatgaccc atcttcaaac acaatggttg ggatgatgcg
     4801 cttcccgttg ttcttttgaa gcacgagggc ttcggcttct ttgtcctgct caatatcgat
     4861 ccacttaaaa gggacaaatt gttcgcccaa aaatttcttc gagcggcggc agtctgggca
     4921 ccaataggca ccatacaggg tgatcttggg ttcagtcatt tcttcctccg gtaatttggt
     4981 atgctctact ggtgcgttgt acgcttgttt ctttactagg acaatcttcc tactcaagat
     5041 gataaaatcc taaaattgct ggctaagggc attggtgagt ccgtggcaag tgatacatac
     5101 aaatcaaaac aggaaggcat ttcaacccat gaatgcaaat caaaacgttg ccgggcagga
     5161 caatattttc atcgctattg cgtccgatgg agaccaaatc aaagatatca ctgctcgagc
     5221 aggtgttttt agtgaagaag aagtcgcctg tgtagcagag atctgggacg attatattga
     5281 aaacggtgtg gaaagttgcg gttataactt tattgtctat cgtgaaggga cgcaggtgct
     5341 gggctttgct tcttatggct accgcgacct gacggatggc gtgtacgatt tgttttggat
     5401 cgcagtagat gccaacaccc gtcgcggcgg agtagggcgc aagttgttgc aagccaccga
     5461 agaagaagtc cgcaaactgg gcgggcgcat ggtgattgcc gaaacttcag gcaaagcctt
     5521 gtatgagtcg acccgt
//
LOCUS       DAOQJE010000109         5507 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_8543_length_5507_cov_7.138114, whole genome shotgun sequence.
ACCESSION   DAOQJE010000109 DAOQJE010000000
VERSION     DAOQJE010000109.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 5507)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5507)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5507
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8543_length_5507_cov_7.138114"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1439
                     /locus_tag="PLT08_04225"
     CDS             <1..1439
                     /locus_tag="PLT08_04225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE03700.1"
                     /translation="FVDARIQAERKAAENKQKSAGKPFFPKEKYSAGDELVFPAVGWQ
                     RGKVASVRPGMNPAVGEFDVITVDFEDSTQRFFAANLPSHALNDAPIEKPGANDINAA
                     AIISEHGSALEKKIESAFGADEGLVKIAGRWFPRALLVDINEGQLNLVEAVLDMAGGE
                     PLTTADLMKDTELPTGVNPKLVEFSLNYAMQYDDRFDEVGPAGQVLWCLRRLEPDFVR
                     DVPPPLRYMDIPHDRSFLSPEMLALEAQLDDELTETDAADRDDVSSVTITLIYPHLRA
                     GTLPVSPRTRKLFPTAYETPRVRFTLVDGKTKQRIPAWVVLEHGYVFGLREWYKAHQL
                     IPGSLVQVRKGENPGEVIVEAKSQRSAKDWVRTVMVGSDGGFVFATLKQPITAEFNDR
                     MVVHVPDFKALDPIWEKKRSFEDLLLMVMRELAKSNPQGHVHAQELYAGVNLVRRVPP
                     APIFAALASNPVFKHVGDLHFRLDEEVN"
     gene            1439..1873
                     /locus_tag="PLT08_04230"
     CDS             1439..1873
                     /locus_tag="PLT08_04230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE03701.1"
                     /translation="MSLLKPTVKTPFHIDFEWWKQNEGDWHIFLRSFLCAEHQEAFAN
                     VEEGEMIDWIDPQTAEVKPVDGVQHALISHCAQLPDFVNQRTAIVEAIFRLFLANGNI
                     PMSAEDLSKKLGKPADTILRTIAGPRVYRGLRPMLNQPTETM"
     gene            1898..1970
                     /locus_tag="PLT08_04235"
     tRNA            1898..1970
                     /locus_tag="PLT08_04235"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1931..1933,aa:Arg,seq:tct)
     gene            2005..2388
                     /locus_tag="PLT08_04240"
     CDS             2005..2388
                     /locus_tag="PLT08_04240"
                     /inference="COORDINATES: protein motif:HMM:NF012241.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HNE03702.1"
                     /translation="MPTVIRKTRETLMEVRRGVLHAVSWQVKSSVWDPPTDLYETEEA
                     YIVRMEIAGMREENFEVSLLNDTLYITGFRHDTPEKRAVHQMEIRSGKFASMVRLPEP
                     VNVEKSLAEYQDGFLTIILPKETNE"
     gene            2401..4980
                     /gene="lon"
                     /locus_tag="PLT08_04245"
     CDS             2401..4980
                     /gene="lon"
                     /locus_tag="PLT08_04245"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HNE03703.1"
                     /translation="MPAQKWQSGLQNFLQMMGENEDEFSNQFMNSFFSQLQKKDDDSN
                     SSQSNGDNEKMSLPDVLPILPLRGVVVYPNTAVPLTVGQARSIKLVDDVVSGDKLVGL
                     VAALNPEQEMPGPNELYRVGTVATVHRLLRAPDGTVRLLVQGMERFRLGEFVEEEPYL
                     KAKIIRAPELDEKNIETDALARNARDQFQAITQMIPSFPEELSSSILSLEDPLQTVYT
                     IANFQRIDLKDAQEILEIDSVYDKLKKLIGLLVREAEVLQIGQKIQNEARGEIEKVQR
                     DYFLREQMKAIQRELGERDEQSEEVDEFRKKIDEAKMPEEADKQARRELDRLSRLPTA
                     AAEYGVIRTYLDTMVSLPWSAGTEDNLDIAHARQVLNQDHYGLEDVKDRILEFLAIRK
                     LRLDRANDKKEESKDQIRHEREGVILCFVGPPGVGKTSLGQSIARAMGRKFIRASLGG
                     VRDEAEIRGHRRTYIGAMPGRILQSLRRVGSKNPVFMLDEVDKLTNDFHGDPSSALLE
                     VLDPEQNGEFRDNYLEVAYDLSQVFFIATANTLEYIPGPLRDRMEIIYLSGYTETEKT
                     QIARGYLIPRQIRENSLRKSEIAFNDEALKLIIRQYTREAGVRNLERKIGAVCRKVGT
                     RIAEGKKKKYNINPKVVEEFLDNPIFLGPEELNKRTSIPGVVPGLAWTSFGGDILLIE
                     ATSMPGGRGFQITGSIGNVMQESARAALSYVRSRSNSFGIAHEYFEKHDIHMHVPAGA
                     QPKDGPSAGVTMATSLVSLITGRKVSPQVGMTGEITLRGQVLPIGGVKEKVLAAHRSG
                     LGTVILPKQNEKDLDDVPDEIRKSMKFVFAETVEDVINSALEKPKQKKKTTKKETKLR
                     KRK"
     gene            4987..5343
                     /locus_tag="PLT08_04250"
     CDS             4987..5343
                     /locus_tag="PLT08_04250"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNE03704.1"
                     /translation="MPKVLLAEDDFTMVSLLKTLLKMEGYEVIALDADADVIAAVRSM
                     KPDVLLLDVHLFSQSGLDILDTLRRSKDTSKVRVLMSSGSNVREECLMRGANGFLMKP
                     YMPDDLFTQLKQVLSV"
BASE COUNT         1418 a         1255 c         1407 g         1427 t
ORIGIN      
        1 agtttgttga tgcacgcatt caagctgaac gcaaggcggc agagaacaaa caaaaatcag
       61 ctggcaaacc atttttccct aaagagaaat attcagcagg cgatgaactg gtcttcccgg
      121 ctgtgggttg gcaacgtggc aaggttgctt ctgtgcgtcc gggtatgaat ccggcggtgg
      181 gtgaattcga tgttatcacc gttgattttg aagatagcac tcaacgattt ttcgcggcga
      241 atttgccttc gcacgctttg aacgatgctc cgatcgaaaa gccgggtgca aatgacatta
      301 atgcggctgc aattattagt gaacatggct ctgctcttga gaagaaaatt gagtctgcat
      361 ttggggctga tgaaggattg gtgaaaattg caggacgttg gttcccacgc gctttgttag
      421 tggatatcaa cgaaggtcaa ttaaatcttg tagaagctgt tttagatatg gcaggcggtg
      481 agcctctaac cactgctgat ttgatgaaag atactgaact gccaactggc gtaaacccta
      541 agctggttga gttttctttg aactatgcga tgcagtacga tgatcgcttt gacgaagtgg
      601 gtcccgccgg gcaggtactt tggtgcttac gccgcctcga accggacttt gtgcgggatg
      661 tccctccgcc gcttcgctac atggacatcc ctcatgaccg cagcttctta tctcctgaaa
      721 tgctcgcgtt ggaagctcaa ctcgacgatg agctaactga gacagatgct gcagatcgcg
      781 atgacgtttc ttccgttacc atcaccttaa tctacccaca ccttcgtgcc gggacgttac
      841 ccgtctcacc gcggacccgc aaactgttcc caaccgcgta tgagacgcca cgtgtgcgtt
      901 ttacccttgt agatggtaaa accaaacaac gcattccggc ttgggttgtt ttggaacacg
      961 gctatgtctt tggtttacgt gaatggtaca aggcgcatca attaatccct ggttcgttgg
     1021 ttcaggtgcg caagggtgag aaccctggcg aagtgattgt ggaagcaaaa agccagcgct
     1081 ctgcaaagga ttgggtgcgc accgtgatgg ttggttcaga tggtggtttt gtctttgcga
     1141 ccctcaaaca gcccatcact gcggaattta atgaccgtat ggtggtgcat gtccctgatt
     1201 tcaaggcgct cgacccgatc tgggaaaaga agcgctcttt tgaggattta ctcttaatgg
     1261 tcatgcgtga attagctaaa tcaaacccgc aaggacacgt ccatgcgcag gaattgtatg
     1321 ctggcgtgaa tcttgtccgc cgtgtgccgc ctgcaccgat ctttgctgct ttggcatcga
     1381 accccgtttt caagcatgtt ggcgatttgc acttccgcct cgatgaagag gtgaactgat
     1441 gagtctctta aagccgaccg ttaaaactcc gtttcacatt gactttgaat ggtggaagca
     1501 aaatgaaggc gattggcata ttttcctgcg ctccttcctt tgtgcagaac atcaggaagc
     1561 gtttgccaat gtggaagaag gcgaaatgat cgactggatc gacccgcaaa ccgccgaggt
     1621 taaaccggtg gatggggttc agcatgcttt gatcagtcac tgtgctcagt tgcccgattt
     1681 cgtcaaccag cggacggcga ttgttgaggc aattttccgc ttgttccttg ctaacggaaa
     1741 tattcccatg tctgctgaag atttaagcaa gaaattgggg aagcctgccg acaccatcct
     1801 gagaactatt gcgggtccgc gggtgtaccg tggtttacgt cccatgttga accagcccac
     1861 tgagaccatg tagtcgggta aaattaaagc aacacaagcc ctcgtagctc aatggacaga
     1921 gcagcagcct tctaagctgt tggttgggag ttcgattctc tccgggggcg ctcgaaagct
     1981 ggcaagatat taaggagtcg tcctatgcct actgtgattc gaaaaacacg cgaaacactt
     2041 atggaggtca ggcgcggcgt cctgcatgca gtgagttggc aggtcaaatc aagcgtctgg
     2101 gatcctccaa ctgacctcta tgagaccgaa gaagcctata ttgtgcgcat ggaaattgca
     2161 ggaatgcgcg aagagaactt cgaggtttca ctgttgaacg atacgcttta catcactggt
     2221 tttcggcacg acaccccaga aaaacgcgca gttcatcaaa tggaaatccg ctctggaaag
     2281 tttgcttcaa tggtgcggct tcctgagcca gtcaatgttg aaaagtcact ggcagaatat
     2341 caagatggtt ttctaaccat catcctccca aaagaaacga acgaataagg ttattactca
     2401 atgcctgcac aaaaatggca atctggcttg caaaattttc ttcaaatgat gggcgaaaac
     2461 gaagatgagt tttcgaacca gttcatgaac tctttctttt cacagcttca gaagaaagat
     2521 gatgactcaa atagctcgca atcgaatggc gacaatgaaa agatgagtct tcctgatgtg
     2581 ttgcccatcc tgcctttgcg tggtgtagtg gtatatccaa ataccgccgt cccgttgacg
     2641 gtggggcagg ctcgttccat caaattggtg gatgacgttg tctctggcga taagcttgtc
     2701 ggtttggtgg cggctcttaa cccagaacag gaaatgcccg gacccaatga actatatcga
     2761 gtcgggacgg ttgctacggt ccatcgtttg ctgcgcgcac cagatggaac tgtccgcttg
     2821 ttggtgcaag gcatggagcg ttttcgtctg ggcgaatttg tcgaagaaga gccttatctc
     2881 aaggcgaaga ttattcgtgc accagagctt gatgaaaaaa atatcgaaac agatgccttg
     2941 gctcgcaatg cacgtgacca gttccaagca attactcaaa tgattccctc ttttccagag
     3001 gaattatcaa gttctatcct ttcattggaa gatccgctcc aaactgttta caccattgcg
     3061 aactttcaac gcattgactt gaaagacgct caagagattc ttgaaatcga ctcagtctac
     3121 gataagctca agaaactgat cggcttgttg gtgagagaag ccgaggtttt gcagatcggg
     3181 caaaagatcc agaatgaggc gcgtggagag attgaaaaag ttcaacgcga ctacttcctc
     3241 cgcgagcaga tgaaagcgat tcaacgggaa ctcggcgagc gtgacgagca atcggaagaa
     3301 gttgatgaat tccggaaaaa gatcgacgag gcgaagatgc ctgaagaggc tgataaacaa
     3361 gcccgacgtg agttggaccg gctctctcgt cttcctactg ctgccgcaga atatggtgtt
     3421 attcgcacct atttggacac gatggtttcg ttgccttggt ctgcaggtac agaagacaat
     3481 cttgacatag cccatgctcg ccaagtattg aaccaggacc attatggttt ggaagatgtc
     3541 aaggaccgta ttcttgaatt ccttgccatt cgtaaattgc gattggatcg tgccaacgat
     3601 aaaaaagaag aatcgaaaga ccagattcgc catgaacgcg aaggtgtgat tttgtgtttt
     3661 gtgggtcctc ctggggttgg caaaacttct ttgggacaat ccattgcccg tgcaatgggg
     3721 cggaagttca ttcgcgcatc attaggtggt gtgcgtgatg aagctgagat tcgtgggcat
     3781 cgacgtacct atatcggtgc catgccgggt cgcattttgc aatcgctgag gcgggtaggc
     3841 tccaagaacc cggtctttat gcttgatgaa gtcgataaac tcacgaacga ctttcacggt
     3901 gacccatctt ctgccttgct ggaagtgctt gaccctgaac agaatggtga gttccgcgat
     3961 aattatcttg aagtagcata tgacctttcg caggtgttct ttattgccac cgcaaataca
     4021 ttggaatata tccccggtcc gctgcgtgac cgtatggaga ttatttatct ttcaggttat
     4081 accgaaactg agaagactca aattgcacgc ggctatctca ttccgcgcca gattcgtgag
     4141 aatagtttac gtaaatctga aattgctttc aatgacgaag ccttgaagtt gattatccgc
     4201 caatatacgc gtgaagctgg ggttcgtaat ttagaacgca aaatcggcgc tgtttgtcgt
     4261 aaggttggta ctcgcattgc agaaggcaag aaaaagaaat acaacatcaa cccaaaagta
     4321 gtggaagaat ttctcgataa ccccatcttc ctcggacctg aagagttgaa taaacgcact
     4381 tcgattcccg gtgttgtgcc cggtttggcg tggacctctt ttgggggtga cattcttttg
     4441 attgaagcca cttccatgcc cggcgggcgc ggtttccaga ttacaggctc gattggaaat
     4501 gttatgcaag aatctgcgcg tgcagctctt tcatacgtcc gctcgcgttc gaactctttt
     4561 gggatcgcgc atgaatattt tgagaagcat gatattcata tgcatgtccc agcaggggct
     4621 caacccaaag acggtccctc cgctggtgtg acgatggcaa cctcactcgt gtcactcatt
     4681 actgggcgaa aggtcagccc gcaggttgga atgaccggtg agattacctt gcgaggtcaa
     4741 gtcttgccaa ttggcggcgt caaagaaaag gtgcttgctg ctcaccgtag cggacttggc
     4801 accgtcatcc tgccaaaaca gaacgaaaaa gatctagacg atgtgccaga cgagattcgt
     4861 aaatctatga aatttgtttt tgctgaaacc gtcgaagatg tcatcaattc ggcgttggaa
     4921 aaaccgaaac agaaaaagaa gacgactaag aaagaaacaa aacttcgaaa aagaaagtaa
     4981 cccagaatgc caaaagtact cctcgccgaa gatgatttca ccatggtttc cttgctcaaa
     5041 actctcctaa aaatggaggg ctatgaggtg attgcccttg atgcggatgc ggatgtgatt
     5101 gcagccgtcc gttctatgaa accggacgtt ctcctgttag atgtccattt gttcagccag
     5161 agcgggttag atatccttga taccttgcgt cgctcaaaag atactagcaa agttcgcgtg
     5221 ttgatgtctt ctggttcaaa cgtccgcgaa gaatgtctga tgcgcggcgc gaatggtttt
     5281 cttatgaaac cctatatgcc agatgacctc ttcacacagc tcaaacaagt cttatcggta
     5341 tgacccaaaa gtttcatata cgagagttca tcttcgatca agattatgag cccgtattga
     5401 ggctttggca aaatattgag tctggcatgc aagttggtcg ttctgactct ccagaagaaa
     5461 tcaaacgtaa agtagagcgc gaccctgatt tgtttttgtt ggctgag
//
LOCUS       DAOQJE010000110         5489 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_8573_length_5489_cov_5.387744, whole genome shotgun sequence.
ACCESSION   DAOQJE010000110 DAOQJE010000000
VERSION     DAOQJE010000110.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 5489)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5489)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5489
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8573_length_5489_cov_5.387744"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..228
                     /locus_tag="PLT08_04255"
     CDS             <1..228
                     /locus_tag="PLT08_04255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE03705.1"
                     /translation="VFNIIERELKAGKSPVIFLHPYEIITPPNYFQRFAKDLLYYPLL
                     IPFSFNKSQFLKDLLKHFPTSTMQDYIKELP"
     gene            225..1247
                     /locus_tag="PLT08_04260"
     CDS             225..1247
                     /locus_tag="PLT08_04260"
                     /inference="COORDINATES: protein motif:HMM:NF024871.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016755 - aminoacyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0044038 - cell wall macromolecule
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan bridge formation glycyltransferase
                     FemA/FemB family protein"
                     /protein_id="HNE03706.1"
                     /translation="MNIQRVDSLNYPALEPQLSYIGLDSWMNFIHDVYGHSVHRFVAL
                     DGDRPLGALSLLEVKSPVFGHYLATAPFGNYGGFAFENHEARNLLLDEAQRLAQDIKA
                     EYVSIRFDDIASPPVDSWVEHPVYSTYLINLSATPDDLLKRFSSDHRNHVRKSLKKGH
                     AIRFGHLDLLNDAYEGLARSMHELGSPYHSKTYLRKMAEYLGNTLEFAVAYDPKGKIT
                     GSGVFIYQGKTIFNLHANVLRFARSSYAGEFLYWSVIEHAMQKGCTTFDLGRSLIGSG
                     NDAFKSKWAPEKKPLAYWYWLAPGHELPSLNQKNPKFQFAIATWKRLPSFIVRPLGPY
                     IIGGLV"
     gene            1252..1851
                     /locus_tag="PLT08_04265"
     CDS             1252..1851
                     /locus_tag="PLT08_04265"
                     /inference="COORDINATES: protein motif:HMM:NF012792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNE03707.1"
                     /translation="MSHTIYPLKQISSDTLPRIAEIHMGDAGLLSKLGHPFVLKYFEY
                     AIKDTRAFGFYAQEDETGEIMGFSLASPEPSSLTSKLTEDKGWFIKNIFKVVFTRPLA
                     FLQMVISSITIRGQMYEPNSIECVYFTVDPKFRGHGLGRSLQKALMDEGRKQGYKKIY
                     ASVETWNIASLKATQANGLKIIKTFREGIYHRHRLESEL"
     gene            1856..2830
                     /locus_tag="PLT08_04270"
     CDS             1856..2830
                     /locus_tag="PLT08_04270"
                     /inference="COORDINATES: protein motif:HMM:NF013381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HNE03708.1"
                     /translation="MTTHRERIQACFEGEQTDRTPIALWRHFPEEDQNPETLAAATIK
                     FQQTYDFDIVKVTPSSSFMVKDWGVDDEWQNNPEGSREYTKRAIQKPQDWETLKLLDP
                     TAPHLAAQLQCLKLVREGIGAETPVIQTVFNPMSQAKNLAGNDLLLEHIKKHPEAVMK
                     GLETITKSTVKFIEAVHEIGADGIFYAIQHAQGRILELDEYKNIALPYDQQVLKPAEE
                     FWCNLLHLHGKDVYFSLLRLMNFQIVNWHDRETYPSLSEAKSLYRGVVCGGIRQETLY
                     SGTPAQVKEEAANAIQQTKGKRFILGTGCVVYYQSAHENIMAAIESTR"
     gene            2827..3381
                     /gene="rsmD"
                     /locus_tag="PLT08_04275"
     CDS             2827..3381
                     /gene="rsmD"
                     /locus_tag="PLT08_04275"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052913 - 16S rRNA
                     (guanine(966)-N(2))-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HNE03709.1"
                     /translation="MSLRVISGTAKGRKLKAVPGDTTRPVMDRVKESLFNILAGDVID
                     SNWWDLFAGTGAIGIEALSRGAAFVKFTDINRMPIETIKDNVETCKFTAQADIRRGDA
                     FTLLAGTADQQFEYIYIAPPQYKKMWAEALNLVDDNMEWLTEDGTVIVQIDPTEYEEI
                     ELHNLVEGEQRKYGGTLLIFYDRK"
     gene            3393..3734
                     /locus_tag="PLT08_04280"
     CDS             3393..3734
                     /locus_tag="PLT08_04280"
                     /inference="COORDINATES: protein motif:HMM:NF025607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4345 family protein"
                     /protein_id="HNE03710.1"
                     /translation="MILTVLQYIACVLTILVGAYSLFAPKSAGKFTGLTPEGGRGITE
                     IRVVLGMFFVALGAYPILFPSDASFHMLGFAYVLVGIARLISIFVDKSGEQSNWISVA
                     SEFILGAILLF"
     gene            complement(3735..3974)
                     /locus_tag="PLT08_04285"
     CDS             complement(3735..3974)
                     /locus_tag="PLT08_04285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE03711.1"
                     /translation="MTDKQSRNETHGCIVCGKLHQMLVVYNVDGTFFDLKVMSSDGKK
                     VVHSTRPLVACERHTKEEVESAVARVYGTQQEDDE"
     gene            complement(4031..4723)
                     /locus_tag="PLT08_04290"
     CDS             complement(4031..4723)
                     /locus_tag="PLT08_04290"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF012387.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HNE03712.1"
                     /translation="MAKKKLAKKNNPQTASSQKTIQYVAGGVILLVVAFFVWQYFPKS
                     APKQDAAICEQFADIPVADQYDSAPPMKVDAAKKYFASVEMENGGQFKMELYPDKAPI
                     TVNSFVFLSCKGFYNGVTFHRVLEGFMAQGGDPTGTGAGGPGYQFVNEDSDLVFDKAG
                     VVAMANAGRDTNGSQFFITFAPADFLNGGYTIFGQVTEGMDVVNAITRRDPQQSPTFE
                     GDKIISVLISEE"
     gene            4805..>5489
                     /locus_tag="PLT08_04295"
     CDS             4805..>5489
                     /locus_tag="PLT08_04295"
                     /inference="COORDINATES: protein motif:HMM:NF025615.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YwiC-like family protein"
                     /protein_id="HNE03713.1"
                     /translation="MRSNSFSCMLAKMQSYFKRQIAFPQDHGSWVFIFSPLMVGLFAG
                     KTFQHATIYLITAALSAFLIRQPITILVKAISGRRSAADLPAARFWLLVYGIIAAAAL
                     LGLIFSGYSYLIYLAIPGAPVFAWHLWLVSKRAERKQLGVEVIATGVLALVAPAAFWV
                     GIGRYDPNGWWFWLFTWLQSAASIVYAYLRLSQREMSPEQAAATGRAEWWKMGIRALL
                     YTSFNMVLAV"
BASE COUNT         1437 a         1333 c         1238 g         1481 t
ORIGIN      
        1 gttttcaaca tcattgaacg tgaactcaaa gcaggcaaaa gtcctgtcat ctttttgcat
       61 ccgtatgaaa ttatcacgcc gccaaattat tttcagcgtt ttgctaaaga cctgctttat
      121 tatccattgc taattccgtt tagcttcaac aaatctcaat tcctcaaaga cctactcaag
      181 catttcccaa cctccactat gcaagattac atcaaagagc ttccatgaac attcaacgag
      241 ttgactctct taactatcca gcccttgaac cgcaattaag ttatatcggg ctcgactcat
      301 ggatgaattt cattcatgat gtgtatgggc attctgtcca tcgttttgtc gctctcgatg
      361 gcgatcgccc attgggcgcg ctttcattgc ttgaggtgaa gagtcccgtt tttggtcatt
      421 atcttgctac agcacctttt ggtaactatg gcggcttcgc atttgagaac catgaagcgc
      481 gcaatctttt gttggatgaa gcccagcgcc ttgcacagga tatcaaagcc gagtacgttt
      541 ctattcgatt tgacgatatc gcctctcccc ccgtcgacag ctgggttgaa caccccgttt
      601 actctaccta tctgataaat ctctctgcca ctccagacga cctgctcaag cgtttctcat
      661 ctgaccatcg caatcacgtt cgcaaatctc tcaaaaaggg acatgccatt cgctttggtc
      721 atctcgacct attgaatgat gcttacgaag gtctcgcaag aagtatgcac gaattaggtt
      781 caccttatca ctccaagact tatcttcgca agatggctga atatcttgga aatacgctcg
      841 aatttgccgt ggcctatgac cctaaaggca aaatcactgg cagtggtgtt ttcatttacc
      901 aaggtaagac catctttaac ttacacgcaa acgtacttcg atttgcacgt tcatcctatg
      961 caggtgaatt cttatattgg tctgtaatcg aacatgccat gcaaaagggg tgcaccactt
     1021 ttgacctcgg tcgcagtttg attggttcag ggaacgacgc attcaaatcc aaatgggcac
     1081 cagagaaaaa gccgcttgct tattggtatt ggcttgcacc ggggcatgaa ttgccatcac
     1141 tcaatcagaa gaatcctaaa ttccaatttg ccattgccac ttggaagcgc cttcccagtt
     1201 tcatcgtccg cccgttggga ccttatatca ttggtggatt ggtgtagtcc tatgtcgcat
     1261 acgatttacc ctcttaaaca aatttcgtct gatacacttc cacgcatcgc tgaaatacat
     1321 atgggtgatg ctggtttgct ctccaagttg gggcatccct ttgtgctcaa gtactttgag
     1381 tacgcaatca aggacacacg cgcctttggt ttttacgctc aagaagatga gacgggtgaa
     1441 attatgggct ttagcctcgc ctctcctgaa ccttcatcac tgacatctaa acttactgaa
     1501 gataaaggct ggttcatcaa gaacatcttc aaagttgttt ttacccgtcc gctagctttt
     1561 ttgcagatgg tcatttccag catcaccatc cgcggacaga tgtatgagcc gaacagtatc
     1621 gaatgtgttt atttcaccgt cgacccgaaa ttccgcgggc acggactggg acgctccctg
     1681 caaaaagccc tcatggacga gggacgtaaa caggggtata aaaaaatcta cgccagcgta
     1741 gaaacttgga acatcgccag cttgaaagcc acgcaagcga atggattgaa aatcatcaag
     1801 acctttagag aaggtattta tcacaggcac cgcctggaaa gcgagttgta agagcatgac
     1861 cacccatcga gaacgcattc aagcctgttt tgaaggtgag cagaccgacc gaactcccat
     1921 cgccctttgg cggcacttcc ctgaagaaga ccaaaatccg gaaacgcttg ccgccgccac
     1981 gattaaattc cagcagacgt atgattttga cattgtaaaa gtcacgccat cctcttcgtt
     2041 tatggtcaaa gactggggcg tagacgacga atggcagaat aaccccgaag gctcgcgaga
     2101 atataccaag cgtgccattc aaaaaccaca ggattgggaa acacttaagc ttcttgaccc
     2161 aactgcgcca cacctcgccg cacaacttca atgcttgaag ctcgtccgcg aagggattgg
     2221 ggcagagacg ccagtgattc aaacggtttt caatcctatg tcgcaggcga aaaatttagc
     2281 aggcaatgac ttgctcttgg aacacatcaa aaaacatcct gaagccgtga tgaagggatt
     2341 ggaaaccatt acaaaaagca cggtcaagtt tatagaagct gtgcatgaaa ttggcgcaga
     2401 tggcatcttc tacgcgattc aacatgcgca aggacggatt ctggaattgg atgagtacaa
     2461 gaatattgct ttgccttatg accagcaagt attaaaaccc gccgaagaat tttggtgcaa
     2521 cttgctccat ttgcatggca aggatgtgta tttctctttg ttgcgcctga tgaattttca
     2581 gatcgttaac tggcacgacc gcgaaacata cccatcgttg agtgaggcga agagcctata
     2641 ccgtggtgtg gtatgcggag gcatccgcca ggagacgctc tactcgggca ccccggctca
     2701 ggtcaaggaa gaggcggcga atgctattca gcagaccaaa ggcaaacgct ttatcctcgg
     2761 cacgggctgc gtagtctact atcaatcagc gcatgaaaat attatggctg ccattgagag
     2821 cacacgatga gcctaagagt gatttcggga actgccaaag gcagaaagtt aaaagcggtg
     2881 ccgggagata ccacgcgccc agtgatggat cgtgtcaaag agtcgctatt caatatttta
     2941 gcaggcgatg tgattgactc aaactggtgg gacttgttcg caggcacagg cgcaatcggt
     3001 atcgaagcgt tgagccgcgg ggctgcattc gtaaagttta cagatataaa tcgcatgccc
     3061 atcgaaacta tcaaagataa tgtagagact tgcaaattca cagcacaagc agacattcgc
     3121 cgtggcgatg catttacgct gctagccggg acagccgatc aacaatttga atatatttat
     3181 attgcgccgc cgcaatataa aaagatgtgg gcagaagcgt tgaatttggt cgatgacaac
     3241 atggaatggc tgaccgaaga tggaaccgtg attgtgcaga tcgacccgac cgaatatgaa
     3301 gaaatcgaat tgcacaatct cgtggaaggg gaacaaagaa aatatggcgg aacgttgttg
     3361 atcttctacg atagaaaata aaaggagcag ccatgatttt gacagtcctt caatatatcg
     3421 cttgtgtgtt gaccattttg gtgggcgctt actctttatt tgccccaaag agcgccggaa
     3481 aatttacagg gcttacccca gaaggcggac gtggcattac tgagattcgc gttgtgcttg
     3541 gaatgttctt tgtagcatta ggagcatatc ccatcctctt cccctctgat gcgtcgtttc
     3601 atatgctcgg attcgcatac gtgctggttg ggattgcaag attgatttca attttcgtgg
     3661 ataaatctgg cgaacaatca aattggatca gtgttgcaag tgaatttatc cttggtgcga
     3721 ttcttttgtt ttagctactc gtcatcttct tgttgtgtac catagactcg tgcaacagca
     3781 gattcaactt cttcttttgt gtgtctttcg caagccacca acgggcgcgt agaatgcaca
     3841 accttctttc catccgagct cattactttg aggtcaaaaa aggttccatc tacattgtag
     3901 acaacaagca tttgatgtag ctttccacaa acgatacagc cgtgtgtttc attacgagat
     3961 tgtttatcgg tcatcatcac tcctaaagaa aagagacagt caccctgaaa gtgactgtct
     4021 cttttttgaa ttactcctct gaaatcaata cagagataat cttatcgcct tcaaaggttg
     4081 ggctttgctg cgggtcacgt cgggtaatcg cattgaccac gtccatgcct tcggtcactt
     4141 gaccaaaaat agtatagccg ccattcaaga aatctgccgg ggcaaaggtg ataaagaact
     4201 ggctgccgtt cgtatcacgc ccagcatttg ccatcgccac cacaccagct ttatcaaaga
     4261 ccaagtcgct atcttcattc acgaactgat aaccaggtcc gcctgcaccg gtgccggttg
     4321 ggtcgccgcc ttgtgccatg aatccttcga gcacacggtg gaaggtcacg ccgttgtaaa
     4381 agcctttgca agacaagaaa acaaagctat tgacagtaat cggcgcttta tcggggtaaa
     4441 gttccatttt gaactgaccg ccattttcca tttcaacact tgcaaaatat tttttggcag
     4501 catccacctt catgggcgga gctgagtcat actggtctgc cacaggaata tcagcaaact
     4561 gttcacaaat cgcggcgtct tgtttagggg cactctttgg aaaatattgc caaacaaaaa
     4621 atgcaaccac aagcaaaatg acaccgccag caacatactg gatagtcttt tgtgaagatg
     4681 cggtttgtgg attatttttc ttagccaact tcttttttgc catacaaatt tctctcctaa
     4741 aaaattttct caattataac aaccttgacc ttctttcctt aataaacaca tgagagatgg
     4801 caccttgcgt tcgaattctt tttcctgtat gcttgccaaa atgcaatcgt acttcaaacg
     4861 gcaaatcgct tttccgcaag atcacggctc atgggttttc attttcagcc cgttaatggt
     4921 cggtcttttt gctggcaaaa cctttcaaca cgcaacgatt tacctcatca ctgcagcgtt
     4981 atctgcattc ctgattcgac aaccaatcac gattctcgtc aaagccatca gtggacggcg
     5041 ctccgctgcg gatcttcccg ccgcccgttt ctggttgctg gtctatggca tcatcgccgc
     5101 tgccgccttg ctcggactta tcttctccgg ctatagttat ctcatctacc tcgccatacc
     5161 gggagcccct gtctttgcat ggcatttgtg gttggtaagt aaacgtgctg agagaaaaca
     5221 actcggcgta gaagttatcg cgaccggtgt cttggctctt gtggcgcctg ccgctttttg
     5281 ggttggcatt ggcaggtatg acccaaatgg ctggtggttt tggttgttca catggctgca
     5341 atcggctgcc agtattgtgt atgcctatct ccggctgagt caaagggaaa tgtcgccaga
     5401 acaggctgcg gctacaggtc gagccgagtg gtggaagatg ggaatccgcg ccttgttgta
     5461 cacatccttc aatatggttc tcgcggtag
//
LOCUS       DAOQJE010000111         5449 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_8671_length_5449_cov_6.154060, whole genome shotgun sequence.
ACCESSION   DAOQJE010000111 DAOQJE010000000
VERSION     DAOQJE010000111.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 5449)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5449)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5449
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8671_length_5449_cov_6.154060"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1004)
                     /locus_tag="PLT08_04300"
     CDS             complement(<1..1004)
                     /locus_tag="PLT08_04300"
                     /inference="COORDINATES: protein motif:HMM:NF013179.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HNE03714.1"
                     /translation="MKKVNHLQHETLTGLHGVIIFSLDIYYQYTGFTESHKQTMKLLW
                     GADIDLGQNILECIQHEDDRKRAQENFDKAIRGELIIVEEEYGDALKHVRLWWENRYS
                     PIYDKDRKIVGVTVFAIDITQRKHIEHALRTSEIHYRSIMDQASDGIFIADPSGRYVD
                     VNPSGCELLGYTREEILNLTMYDIAVGTKFNPIRIKELMDGKVILTERELLGKNGKLV
                     PVEISAKKMEDGHLMGIIRNIEARRQEEARVRFLAFVMDKISSAVISTDTDMKINYWN
                     KSAENLYGWKEEEVLGQNIDEVCGTEFLEGQQELAKNALLYEKTWRGELKQNHRNGKE
                     "
     gene            1219..2484
                     /gene="obgE"
                     /locus_tag="PLT08_04305"
     CDS             1219..2484
                     /gene="obgE"
                     /locus_tag="PLT08_04305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HNE03715.1"
                     /translation="MFIDQVNIHVKSGKGGDGMVHFRREKYEPRGGPDGGDGGKGGDV
                     IFEVKPILNSLSHFKPKQSFAAENGINGGGSQMTGRGGKDLTIHVPPGTVIYDAETGA
                     LLGDLTDPGQQLIVCKGGRGGRGNQHFATSRNQAPRTAERGEPHEEKMLRLELKLIAD
                     IGIIGLPNAGKSTLLSVLTNAKPKIGDYPFTTLEPNLGVAKIDEEATVVLADIPGLIE
                     GAHEGAGLGHDFLRHIQRTRVLIHMIDGLSEDPLADFSQINTELSLFDTKLGKKPQVV
                     VINKIDQPDVQARLKEIQTSFKKKKVEIITASAMARNNTRDVLLTAYRMLQELPPEQL
                     GEEALPVYKPEVDPNLFDIHREDGDKWRVSGVAIERAAKMTYWEHFGSVRRFQRLMQK
                     LGVDKALREAGVQDGDTVFVGDFELEWQE"
     gene            2575..3003
                     /locus_tag="PLT08_04310"
     CDS             2575..3003
                     /locus_tag="PLT08_04310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE03716.1"
                     /translation="MTKQRILTTGLIVIGLVVVIFFGMNTLRAFKHMRGHGPFNGKPP
                     TANQTDVELIRDWMTLPYIGSMYDVPPEALFYSLGIEPGKQNGKKSLKELNDEFFPEQ
                     PDLAISQVKDTIKAFQTQERPPEPFPPVAPTPPSPPTQNP"
     gene            3000..3611
                     /locus_tag="PLT08_04315"
     CDS             3000..3611
                     /locus_tag="PLT08_04315"
                     /inference="COORDINATES: protein motif:HMM:NF020893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HNE03717.1"
                     /translation="MTDTISGFFLTQVINYGAPLFALTLFLGAVGLPVGASLMVIAVG
                     AFSQQGYFEWTTMALLGLLGAALGDALSFGMGHYGKGWVEKRFGTSPTWKNAQSYFDK
                     RAWVAIFLTRFLVTALAIPTNLIAGGSGYKFQRFMLYDVLGEMTWIILYGGLGYAFGS
                     QWEVVYDFIGNFGGFVLGAVILGAGIWMARNQFNKPKQEQLAV"
     gene            complement(3645..4136)
                     /locus_tag="PLT08_04320"
     CDS             complement(3645..4136)
                     /locus_tag="PLT08_04320"
                     /inference="COORDINATES: protein motif:HMM:NF012478.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HNE03718.1"
                     /translation="MANDTVKDGLVVSMEYKLTVDGEVLDSSDEAGPLQFLAGYGNII
                     PGLENEMMGMKIGDSKNVTVQPADGYGEFEDDAFVDVPRTEFPADMEIEEGMELHVTD
                     EDGNHQAAYVAEFDDKTVKLDFNHPLAGAVLDFYVKVVALREPTDEELDHGHVHEGDG
                     HHH"
     gene            4258..5325
                     /locus_tag="PLT08_04325"
     CDS             4258..5325
                     /locus_tag="PLT08_04325"
                     /inference="COORDINATES: protein motif:HMM:NF015535.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P1 family peptidase"
                     /protein_id="HNE03719.1"
                     /translation="MKKIRARDLGIPFEGTPGAFNAITDVPGITVGYSTIIEGTDART
                     GVTIIHPRSMDNPTPVFAGVHSFNGNGEMTGAAWVDEGGILEGPIGLTNTHSVGVVHH
                     ALIKWQVEHHKLTQNWLTPVVAETADAWLNNMNGFFVQEHHVLEAINSAKSGEIDEGN
                     VGGGTGMLCYEFKGGTGTSSRKLPEKLGGWTVGAIAQCNFGRRYQLTIAGVPIGHHLT
                     DDAPATNGENPFTQDDGSLIVIIATDAPLLPHQLKRVAKRVSLGMSRTGAIGGNGSGD
                     IFLAFSTANSEAAKGLGRTQLEALPNGQLDPIFAATAYATEEAIVNALVAAEDMTGHE
                     ISAKALPHEDVKELLKKHGRL"
BASE COUNT         1503 a         1287 c         1189 g         1470 t
ORIGIN      
        1 atttctttgc cattgcgatg gttttgtttt aattccccgc gccaggtctt ttcgtacaaa
       61 agggcatttt tggcaagttc ttgttgccct tctaaaaact ctgttccaca cacttcgtca
      121 atattttgtc ccaatacttc ttcttctttc cacccatata aattttctgc gcttttattc
      181 caataattga ttttcatgtc tgtatcagtt gaaatcactg cactagaaat tttatccatt
      241 acaaaagcaa gaaaacgaac acgcgcctct tcttgccttc gtgcctcaat gtttcgaatg
      301 atccccataa ggtgtccatc ttccattttc tttgcactaa tctcaacagg caccaatttc
      361 ccattttttc cgagtaattc ccgctcggta agaatgacct taccatccat caattctttt
      421 attcgaatag gattgaattt tgttcccact gcaatatcat acatggtcag attaaggatt
      481 tcttcacggg tgtaccccaa cagttcacac ccagaaggat tcacatccac atatcgacct
      541 gatgggtcag caataaaaat cccgtcagat gcttgatcca taatagaccg ataatggatc
      601 tcactagtgc gtagcgcatg ttcaatatgt tttctctgtg taatatcaat tgcaaaaaca
      661 gtaacgccaa caatttttct atccttatca tatattggcg aataacgatt ctcccaccac
      721 aaacggacat gtttcaatgc atccccatat tcttcttcta caatgattaa ctcaccacgt
      781 atggctttat cgaaattctc ttgcgccctt tttcggtcat cttcatgttg aatacattca
      841 agaatatttt gtccaaggtc aatgtctgcc ccccaaagca atttcatggt ctgtttatga
      901 gactctgtaa atcccgtata ttgataataa atatcaagag aaaaaataat aacgccatgc
      961 aagcctgtaa gagtttcatg ctgaagatgg tttacctttt tcatcttaac aatcatcttt
     1021 caataattaa aactaaaggg tgctgcggcc ttgtttgtat actactaaac gtatacaaac
     1081 aagagattat ttttatccta gcatcattct acatctcaat taatagccat ttaccttatc
     1141 tatcatgagt ctattcaagc aaatacccct taatttgtat tccctcgaaa gatcacttat
     1201 agccattata atgggggaat gttcattgac caagtaaata ttcatgtaaa atctggaaaa
     1261 ggcggcgatg gtatggtgca ttttcgccgt gaaaaatacg aacctagggg cggtccggac
     1321 ggcggagacg ggggcaaagg cggcgatgtc atctttgaag tgaaacccat tcttaattcc
     1381 ctctctcact ttaaacccaa gcaatctttt gcagccgaaa atggcatcaa cggcggcggt
     1441 tcgcaaatga ccgggcgtgg cggcaaagac ctgaccattc atgttccacc gggaaccgtc
     1501 atctatgatg cggaaaccgg agcattgctc ggcgacctca cagacccagg acagcaactc
     1561 atcgtctgca agggcgggcg gggtggacgt ggcaaccagc attttgccac ctcacgaaac
     1621 caagccccaa ggaccgccga acgcggcgaa cctcatgagg aaaaaatgct gcggctcgaa
     1681 cttaagctga ttgctgatat tggcatcatt ggcttaccca acgccgggaa atccactctc
     1741 ctctccgtgc tgacgaacgc caaacccaag atcggtgact atcccttcac aactctcgaa
     1801 cccaacctgg gtgttgccaa aattgatgaa gaagccaccg ttgtgctggc agatatccct
     1861 ggtttgatcg aaggcgctca tgaaggtgca gggttggggc acgacttttt acgccatatt
     1921 caacgcactc gcgtcctcat tcatatgatt gacgggttat cagaagaccc actcgccgat
     1981 ttcagtcaga tcaatactga gctttcgctc tttgatacaa agctaggcaa aaagccgcag
     2041 gttgtggtta tcaataagat tgaccagcct gatgtgcaag cgcgattaaa agaaattcaa
     2101 accagcttca aaaagaaaaa agtggaaatc atcactgcct cagccatggc acgcaacaac
     2161 acacgagatg tgttgctcac agcgtatcgt atgcttcagg aactgccacc agaacaactc
     2221 ggggaagagg cgttgcccgt ctacaaaccg gaagtagacc cgaacttgtt cgacatccat
     2281 agagaagatg gagataagtg gagagtatcg ggggtagcga ttgaacgtgc tgcaaagatg
     2341 acgtattggg aacactttgg ctctgtgcgg cgcttccaac gtttgatgca aaaactcggc
     2401 gtggataagg cattacgcga agccggggtt caagatggcg atacggtttt cgtaggtgat
     2461 tttgaattgg aatggcaaga gtaacgataa aaggacacac cacatggtgt gtcctttttg
     2521 attccacatt atttctttac aatttgaacc tgaaagcagg gataaaatac gcatatgact
     2581 aaacaacgca ttttaacaac cgggttgatc gtcataggat tggttgtcgt tatctttttt
     2641 ggcatgaata ctcttcgcgc ctttaaacac atgcgcggac atggtccatt caatggcaaa
     2701 ccacctacag cgaatcaaac tgatgtggaa ttgatccgag attggatgac gcttccctac
     2761 attggcagta tgtatgatgt tccgccagaa gccttgttct acagtttagg gattgaaccc
     2821 ggaaaacaaa acgggaagaa aagtttgaaa gaattgaacg atgaattctt ccctgaacaa
     2881 ccagacctgg cgatctcgca agtaaaagat acgatcaagg cattccaaac acaggaacgt
     2941 cctccggaac cattcccacc tgttgcaccg actccaccgt caccgccgac tcaaaaccca
     3001 tgaccgatac gatctctgga tttttcctta cacaagttat taactacggg gcgcctcttt
     3061 ttgcgctgac gctttttctt ggagcagttg ggctacccgt aggtgcctct ctgatggtta
     3121 ttgctgttgg cgctttctct caacaaggat atttcgaatg gaccacgatg gcgcttctgg
     3181 gcctgctcgg ggcagcactt ggagatgctc tcagctttgg aatgggtcat tatggaaaag
     3241 gctgggttga aaagcgcttt ggcacctccc ccacttggaa gaacgctcaa tcctattttg
     3301 ataaacgtgc ctgggtagcc atttttctca ctcgcttttt ggtaacggcg ttggcaattc
     3361 ccacaaacct gattgctggt ggcagcggat ataagtttca acgttttatg ctatacgatg
     3421 ttctcggcga aatgacctgg atcattctgt atggagggtt gggatatgca tttgggtctc
     3481 aatgggaagt ggtctatgat tttattggaa acttcggtgg gttcgtatta ggtgccgtga
     3541 tcttaggtgc tggcatttgg atggcaagaa atcaattcaa caaacccaaa caagaacaac
     3601 tagctgtata aaaaaacatc ctctatacag aggatgtttt tttattagtg atgatgtcca
     3661 tcgccctcgt ggacatgacc gtgatcgagt tcttcgtccg ttggctcacg gagagctaca
     3721 actttgacat agaaatccaa cacagcgcca gcaaggggat gattgaaatc aagcttgacg
     3781 gtcttgtcat cgaattcagc cacatacgca gcctgatgat taccatcttc atcggtaacg
     3841 tgcaattcca taccttcttc tatttccata tcagcaggga attcagtgcg tggaacatca
     3901 acgaaagcat cgtcttcgaa ctcgccatat ccatcggctg gttgaactgt gacatttttg
     3961 ctgtcgccga tcttcattcc catcatttca ttttccaaac cgggaatgat attgccgtac
     4021 cccgccagaa actgcaaagg acctgcttcg tcagaagagt ccaaaacttc gccgtctacg
     4081 gtcagtttgt attccatgga aaccaccaaa ccgtctttta ctgtatcgtt tgccatactt
     4141 gtatcctttc gaatttaatg ttcccattgt accagcaata aatagactgc aaggcatcca
     4201 cagaacccct tcggctataa tgttggcgtc ttttcaatgc tttgccaagg agaatgtatg
     4261 aaaaaaatac gtgcccgcga tctgggcatc ccttttgagg gaacacctgg ggcattcaac
     4321 gcaatcacag acgtgcctgg tattacggtt ggatactcca ctattatcga agggacagat
     4381 gcacgcaccg gtgtgacgat cattcatcca cgcagtatgg ataacccaac cccggtattc
     4441 gccggagtcc attcttttaa tggcaatggt gagatgaccg gggctgcctg ggtagatgag
     4501 ggcggtattt tggaaggtcc tattggatta acaaacacgc atagcgttgg cgttgttcat
     4561 catgccttga tcaagtggca ggttgaacac cataaattaa ctcaaaattg gttaaccccc
     4621 gttgtagctg aaaccgcaga tgcctggctc aataacatga atggtttttt cgtacaagaa
     4681 caccatgttc ttgaggcgat caactctgcc aaatcgggtg agatcgatga aggcaacgta
     4741 ggcggtggga cgggtatgct atgctacgaa ttcaagggcg gaacgggaac atcatccaga
     4801 aaactgcccg aaaaacttgg cggttggaca gtcggcgcga tagcgcaatg taactttggc
     4861 agacgttatc aacttacgat tgcaggcgta cctattgggc atcacctaac agatgacgcc
     4921 cctgccacca atggtgaaaa tccgtttacg caggatgatg gttcattgat cgtcatcatt
     4981 gcaaccgacg ccccacttct accacatcaa ctgaaacgtg tcgcaaagcg ggtctctctt
     5041 ggaatgagtc gtacgggcgc aattggcgga aatggctctg gtgatatttt ccttgcattt
     5101 tccacagcga attctgaagc ggcaaaggga ctgggacgga cccagcttga ggcattgcca
     5161 aacggtcaac ttgacccgat ctttgccgcc acagcttatg caacagaaga agcgatcgtc
     5221 aacgcgcttg ttgcagctga agatatgaca gggcatgaga tcagcgccaa ggctttgcca
     5281 catgaagatg tgaaagaact cttgaagaaa cacggcaggt tataaatcag taaatccgct
     5341 taactgacta tacgaaaatt ctataaaccc tagtcgttta tgactagggt ttgtagttta
     5401 agagcccttg acaaaacaaa acctataata tataaacagt tatatataa
//
LOCUS       DAOQJE010000112         5437 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_8693_length_5437_cov_5.728725, whole genome shotgun sequence.
ACCESSION   DAOQJE010000112 DAOQJE010000000
VERSION     DAOQJE010000112.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 5437)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5437)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5437
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8693_length_5437_cov_5.728725"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..323
                     /locus_tag="PLT08_04330"
     CDS             <1..323
                     /locus_tag="PLT08_04330"
                     /inference="COORDINATES: protein motif:HMM:NF019886.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HNE03720.1"
                     /translation="TIARNLAYKKSQREKRFVPLSADLVERQAEPSQLAEQAMNLRDA
                     VQFIQTLPEIDRTALVLRLQNELTYEEISSILNISLAAAKVKVHRARMKLTEYMRHQE
                     ELSQ"
     gene            320..733
                     /locus_tag="PLT08_04335"
     CDS             320..733
                     /locus_tag="PLT08_04335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE03721.1"
                     /translation="MKITRDVINDLLPVYQSGEASEDTQRLVEEFLQTDPEFAELVAV
                     QDQPLEKLNINISKETEMKTLQDTRALLQKRSIYLAFTIFFFLLPASFTFDSNGIYWM
                     WAKAPINVLIFVAFGIFNAVQYWRTSQKLKGSGLE"
     gene            complement(778..1182)
                     /locus_tag="PLT08_04340"
     CDS             complement(778..1182)
                     /locus_tag="PLT08_04340"
                     /inference="COORDINATES: protein motif:HMM:NF020950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease inhibitor I42 family protein"
                     /protein_id="HNE03722.1"
                     /translation="MKNTLYLLIASVLFLAACASPTPAPTADPASSTMPALTDPSKPI
                     TVSAGETFMIVVESNPSTGYHWEIVGDLTNVEFVSREYTGSEPVIPGSGGVEVWTFKA
                     TTAGDTSFVLGNYPPGEGTTYEQQVQFSVTVQ"
     gene            complement(1279..1644)
                     /locus_tag="PLT08_04345"
     CDS             complement(1279..1644)
                     /locus_tag="PLT08_04345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE03723.1"
                     /translation="MEIKHLGDPVLNDILSSNVESFSMIETVPHEVVLIHNGQGYNVE
                     LGLLSVGKVHDSVYSKFPPVPKNLKFVVIILSQRATRAMMGSALEILDRVFFRRKMTA
                     VVASMQEAEAQIMQMGFSG"
     gene            complement(1733..3667)
                     /locus_tag="PLT08_04350"
     CDS             complement(1733..3667)
                     /locus_tag="PLT08_04350"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:NF028237.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HNE03724.1"
                     /translation="MFKFTVIAATLLITASLNIISTTISWSRAKSRIGLYFAIGMTGV
                     TFWTLASGLDYAAIPIPLKIYFAKWEYVFYHITLVFFLMFIMSFAGYKTLLKNKLVIT
                     VLWVIPTINILLAWTNDWTGWLWVGFVESNFGNNTIIFEHGFAFLWVTATGYMLLASI
                     IAASWIAMRRSFGFARRQGQVLFYGSLLPLIGNLIYLFQPPAFDGVDWSSIFISGSSV
                     LFLWAFYGTNLINVIPIAREKLIDRSGDGMIVLDSRNYIIDINESAAKLLNLTVASLL
                     GKKLADYLPDAKFLSREALEEELSTELEIEAPTPRFFDVLVTPLFEGQKTIIGSLVVL
                     RDITKRRQNELRLLLLNQTVEQSPNSVVITDFDGKITYVNSAFTAITGYSAKEVLGKK
                     TSILKSGYMSKEVYENLWQTILSGKTWEGELNNMKKGGELFWENTRIAPLLNQDGEIS
                     NFVAIKVDITERKRAQDELERMAITDPLTGLFNRRYFFEVAQKEFLKSIRYNRPLSVV
                     LFDIDMFKSINDTYGHLAGDQILAQIGVLLTQKERETDLSARYGGEEFVLLLPETDCE
                     GARNTAERLRSLLETSPIYYENHKIQFTASFGVAGIGNRDYPETFDYLILQADKALYE
                     AKRISRNTVICSCETQSEIK"
     gene            complement(3828..4316)
                     /locus_tag="PLT08_04355"
     CDS             complement(3828..4316)
                     /locus_tag="PLT08_04355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE03725.1"
                     /translation="MSSNPRKAKRQQQIRIFNALIGFCAIFARVPKLKPISNFLLRRL
                     ADFTYWMRNGQQQNDLTGLAKEWQRMFPSRAFVPIKQVTAETAFAEIHGVCPVTASGD
                     VHACHRLMEYDRHLLKKMGGQFVVLDSKADPTVPICMVAMRMKERSIEDLIPAHSKSP
                     LQ"
     gene            complement(4566..>5437)
                     /locus_tag="PLT08_04360"
     CDS             complement(4566..>5437)
                     /locus_tag="PLT08_04360"
                     /inference="COORDINATES: protein motif:HMM:NF025658.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF4375 domain-containing protein"
                     /protein_id="HNE03726.1"
                     /translation="GNGNFDQFYYNSTGDYSLEMVSALEEIGAYDAARILSECNRIFP
                     NGVPPQNRDERIQALDALPSNSIAFLNRAGHELMDDSDFLLRIYHYYVTGEKISAAEL
                     RTVYYNFYAGERFFQDLSKRPENEQFDFLLKKIKEMMMVPFSERYFLDKRIFEIRTYL
                     LNLAYFDEKAIVEIIERFIYKPGFNKQGSKAFDDFGAFTREADLTLELLKILERKAEP
                     SDNLEIKLQDWFVKLLDVPAEKSMIWLNLKYVAVVLHSLWETKYPEPIIGPSDNKLLN
                     AEVYLKAINSHLK"
BASE COUNT         1546 a         1139 c         1170 g         1582 t
ORIGIN      
        1 tgacgattgc tagaaatttg gcgtacaaaa agtcgcagcg tgagaagcga tttgttcctt
       61 tgagcgcgga cttggtcgaa cggcaggcgg aaccgtcgca attggctgag caagctatga
      121 atttgcgaga tgcggtgcaa tttatccaaa ccttaccaga aatagaccgc acagccctgg
      181 tattacggtt gcaaaatgaa ttaacgtatg aagagatctc cagcatattg aatatttcac
      241 tggcggctgc gaaggtcaaa gtccatcggg cgcggatgaa gttgaccgaa tatatgcgcc
      301 accaagagga gttatcacaa tgaaaatcac acgcgatgtc atcaatgacc tgttacctgt
      361 ttatcagtca ggagaagcca gcgaagatac acagaggctt gtggaagaat tccttcaaac
      421 cgaccctgaa tttgcagaac tcgttgcggt gcaagaccag ccgcttgaga aacttaatat
      481 caatatatca aaggagactg aaatgaaaac actgcaagat actcgtgcac tattacaaaa
      541 acgctcgatc tacctggcgt tcactatctt tttcttcttg ctgccagctt catttacatt
      601 tgactcgaac ggcatttact ggatgtgggc aaaggctccg atcaacgttt tgatctttgt
      661 cgctttcgga atctttaatg cggttcaata ctggcgaact tcgcagaagc tcaaaggaag
      721 cggattggaa taagcagtag taaaaaacac ctcctatttc taggaggtgt tttttattta
      781 ttgtaccgtg acggaaaact gtacttgctg ttcgtatgtt gtgccttccc cgggagggta
      841 atttccaagc acaaatgagg tatctccagc agtagtagct ttgaaggtcc agacttctac
      901 gccgccagag ccggggatga cgggttctga gccggtatat tcacgtgaga caaattcaac
      961 attggtcaag tcccctacga tttcccagtg atagcccgtg gacgggttgc tttcgacgac
     1021 gatcatgaag gtttcaccgg cgctgactgt gattggctta gatggatcgg taagggcagg
     1081 catagtggaa gatgccgggt ctgcggtggg agcaggggta gggctggcgc aggctgctaa
     1141 aaatagtacg gaggcgatta ataaatataa ggtgtttttc atgtaaggct cctttggttt
     1201 atagggagat aacgttcaat atgaaaattt tgttcccggt taaagagaaa aagatgcaaa
     1261 tgtaaggatt gtcttcttct agcctgaaaa ccccatttgc ataatctgag cttctgcctc
     1321 ttgcatggat gcgaccacgg ctgtcatctt gcgtcggaag aagaccctat ccaatatttc
     1381 caaggcggag cccatcatgg cacgggtggc acgttgactc aagatgatga ctacgaattt
     1441 caggtttttt ggaacgggtg gaaatttgct atacaccgag tcgtggacct tgccaacgct
     1501 gagcagaccc agctctacgt tgtagccttg accgttgtga atgagcacga cttcatgcgg
     1561 cacggtctcg atcatcgaga acgattcgac atttgacgaa aggatgtcat tcaagaccgg
     1621 atcgccgaga tgtttgattt ccaaaagata tgaatttatc ttttcagcgt acggtacttc
     1681 atacgatgcg tggtgaggtc tttgtcgaat ttttcttcat caccgttcaa attcatttga
     1741 tttccgattg tgtttcacaa gaacaaatga ccgtatttct actgattcgc ttggcttcat
     1801 ataaagcttt atcagcttgt aaaattaaat aatcaaaagt ttctggataa tctcgattac
     1861 caataccagc taccccaaaa cttgcggtga attggatttt atgattttca taataaatag
     1921 gggaagtttc caacaagcta cgcaatctct ctgcagtatt tctagcgcct tcacaatctg
     1981 tttcaggtaa taataaaacg aattcctctc ccccatatcg agcagaaaga tctgtttcac
     2041 gttccttttg ggtcagcaga accccaattt gagccagtat ctgatcacca gcaaggtgtc
     2101 catacgtgtc attgatactt ttgaacatat caatatcaaa taaaaccaca gatagaggtc
     2161 tattatagcg aatagatttt agaaattctt tctgagcgac ttcaaaaaaa taacgacgat
     2221 tgaacaaccc ggtaagaggg tctgtgatag ccatacgctc caattcatcc tgagctcgtt
     2281 tgcgttcagt aatatccact ttgatggcga caaagttact gatttctcca tcctgattca
     2341 gtaatggcgc gatgcgggta ttttcccaaa aaagctcgcc accttttttc atattattca
     2401 gttcaccttc ccaagttttc ccagacagaa tcgtttgcca aagattttca tatacttctt
     2461 ttgacatata tccagatttc agaatggatg ttttttttcc aagcacttcc tttgctgaat
     2521 aaccagtgat ggcagtaaat gctgaattga catatgtgat tttgccatcg aagtcggtga
     2581 ttacaactga atttggactt tgttctactg tttgattaag caaaagcagg cgtagttcat
     2641 tttgtctgcg tttggtaata tctcttaaaa cgaccagaga tcctattatc gttttctgtc
     2701 cctcaaataa aggggtgaca aggacatcaa agaaacgtgg ggtgggcgct tcgatctcta
     2761 attctgtact taattcttct tccagtgctt ctcttgacaa gaatttggca tcaggcaaat
     2821 agtcagccag ttttttacct aataggcttg caactgttaa atttagtagt tttgctgcgg
     2881 actcgttgat atcaataata taatttcttg aatccaagac aatcattcca tcccctgacc
     2941 gatcaatcaa tttttcacga gcgattggaa tgacatttat taaatttgtg ccataaaatg
     3001 cccaaagaaa taagacactt gacccagaaa taaaaatact tgaccaatct acgccatcga
     3061 atgcgggggg ttgaaataag tagataaggt ttccaataag tggtaggagg ctgccataaa
     3121 atagaacttg tccctgtcga cgtgcaaacc caaaacttct acgcattgca atccacgaag
     3181 cggcaataat acttgccaat aacatatagc ctgtcgcggt tacccacaag aacgcaaatc
     3241 catgttcaaa aataattgta ttattcccga agttgctttc aacaaatcca acccataacc
     3301 aaccagtcca gtcattcgtc catgcaagta ggatgttaat agtaggtatc acccacaaca
     3361 ctgtaatgac caatttattc ttcaataatg tcttataccc cgcaaacgac atgataaaca
     3421 tcaagaaaaa gaccagtgta atgtgataaa aaacgtattc ccatttagca aaataaattt
     3481 ttaaggggat tgggatggct gcataatcaa gcccggatgc caatgtccaa aaagtaaccc
     3541 cggtcatccc tatggcaaag taaagtccaa tgcgagattt tgctctactc caactaatag
     3601 ttgttgaaat gatatttaga cttgctgtaa ttaatagggt tgcggcaata acggtaaatt
     3661 tgaacataat ctcttgcaat agtttacatg atttaaaaga aattacaaaa cttcatatcc
     3721 ccttagaaat caaaaggaac ggatgcgaga cccgttccta cgattatctt ttcaacgtgc
     3781 ggtatttgac tcgatacgtg gtcaaatatc tgccgatggc attttattta ctgcagcgga
     3841 gatttggaat gcgcggggat gaggtcttcg atggagcgtt ctttcatgcg cattgcaacc
     3901 atgcaaatgg gaacggtggg atctgccttg gagtcgagca cgacgaattg cccgcccatt
     3961 tttttgagga gatgcctgtc gtattccatc aggcggtgac aggcgtggac atctccgctg
     4021 gcagtgacgg ggcaaacgcc gtgaatttcg gcgaaggctg tttcagcagt cacttgtttg
     4081 atcggtacga acgcccgact ggggaacatg cgctgccatt ctttggcgag accagtcaga
     4141 tcgttttgct gttgcccgtt gcgcatccaa taggtgaagt ctgcgagacg gcgcaggaga
     4201 aagtttgaga tcggtttcag cttgggaaca cgcgcaaaaa tcgcgcagaa tccgatcagg
     4261 gcgttgaaga ttcggatctg ttgctggcgt tttgctttgc gtgggttgga ggacatgttg
     4321 aaattttatg gggatatgag aatgtagggg cgacacactg cgcagcgtcg cccttgatgt
     4381 tgagatgaaa caaaaagggg cgggggactc tgcccctacg gacgcttatc tcttcagcgt
     4441 tctatatttc acccgatgtg gagttaagtc tttgccaaat ttttcggtca tcatcacttc
     4501 ataatctgac cagttgccaa gatgtactct tgtggcaatg tcactttcaa aggtgaagat
     4561 aacttttatt tgagatgtga attgattgct ttcaagtaaa cctcggcatt tagcaatttg
     4621 ttgtcacttg gcccaattat gggttcaggg tatttggttt cccataaaga gtgaagcact
     4681 acagcgacat attttaggtt taaccaaatc atgctctttt ctgctggcac atctaaaagc
     4741 ttaacaaacc aatcttgaag tttgatttcc aggttatcag atggctctgc ttttcgctcc
     4801 aatattttta gaagttcaag agttaaatct gcctctcgtg taaatgcacc aaaatcatca
     4861 aaagctttgc ttccttgctt gttaaatcca ggcttgtaaa taaagcgctc aataatttcg
     4921 acaatagctt tttcatcgaa atacgccaaa ttcaaaagat aagttcttat ttcaaaaatt
     4981 cttttatcta aaaaatagcg ttcactaaat ggcaccatca tcatctcttt gattttcttt
     5041 aagagaaaat caaattgctc attttctggt ctttttgata agtcttggaa aaagcgctca
     5101 ccagcataaa aattatagta tacagttctg agttccgctg ctgatatttt ttctccagtc
     5161 acataatagt gataaattct cagcaaaaaa tctgaatcgt ccattagctc atgacctgct
     5221 ctgtttaaga atgcgattga gtttgatggc aaagcgtcaa gtgcttgaat tcgttcatct
     5281 ctgttttggg gcggcacccc atttgggaat atcctattgc actcagataa aattctcgcc
     5341 gcatcatacg cccctatttc ttccaaagcg gaaaccattt caagtgaata atcgcctgtg
     5401 gagttgtaat aaaactggtc gaaattacca ttgccga
//
LOCUS       DAOQJE010000113         5419 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_8732_length_5419_cov_5.598620, whole genome shotgun sequence.
ACCESSION   DAOQJE010000113 DAOQJE010000000
VERSION     DAOQJE010000113.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 5419)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5419)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5419
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8732_length_5419_cov_5.598620"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..594
                     /locus_tag="PLT08_04365"
     CDS             <1..594
                     /locus_tag="PLT08_04365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase"
                     /protein_id="HNE03727.1"
                     /translation="LATPHGVVLQQPAFSKYYLHLGEISSYPPGYKENAGIFCHTNPW
                     IMIAEAKVGRGDKALDYYLRINPSAREGIGDLHRCEPYVYAQMIAGKDAATHGEAKNS
                     WLTGTAAWNYVAITQWILGIRPTYEGLQVSPVVPVGWKSFEAVREFRSVRYNIKVERS
                     GAGNQVKLEVDGKAIAGNIIPLAANGTKEVRVRVILS"
     gene            complement(642..1343)
                     /locus_tag="PLT08_04370"
     CDS             complement(642..1343)
                     /locus_tag="PLT08_04370"
                     /inference="COORDINATES: protein motif:HMM:NF015956.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HNE03728.1"
                     /translation="MSVSLAMQRYAAEIYRLQQDHEQVPLSLLSSHVDASAQAISNMV
                     KRLQKLGYLVHEPYRGVRLTETGEKIAMPSLRRHRLTEVFLVKVMKYDWATAHELSDT
                     FEKGVNDEIENRMDELAGRPTRCPHGEPIPSKDGFMPVVKDMPLIDVPSGSDCVISRV
                     RTHDLEKLNYIGELGLTPGKQFHLFSCAPFKGPLRLQMKPHDHLIGYELAASLWVEVT
                     MKGEGNKVPTSLASK"
     gene            1507..2046
                     /locus_tag="PLT08_04375"
     CDS             1507..2046
                     /locus_tag="PLT08_04375"
                     /inference="COORDINATES: protein motif:HMM:NF017987.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ChuX/HutX family heme-like substrate-binding
                     protein"
                     /protein_id="HNE03729.1"
                     /translation="MFQTNISLSPEMTANERNQAIREAYNQDRSQMTLMLAHQLRIPE
                     VDILRALEGDTARELDFARWEEIIRAFEALGEVTVIVSNGSTTIESFGQFGGFSNAQG
                     FLNVRSKSLDMHIRGWELASVFAFRKPSHLDKHESLSFQFFDKRGNSAFKVFLNFGGK
                     DPASELVEAYNDLIGRFSK"
     gene            2186..3490
                     /locus_tag="PLT08_04380"
     CDS             2186..3490
                     /locus_tag="PLT08_04380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BCD family MFS transporter"
                     /protein_id="HNE03730.1"
                     /translation="MFIKRLQLALIHTAVAMTLVPINSTLNRVMIFDLGVSKTLFTLL
                     AIFPYLLSPIQVAIGSFSDRHPILGYRRAPYIFAGLLLCVIGVAVSPQIAILISNNFT
                     VGVAAGILAFGLWGMGYNLSAVSYLSLASELSGEKERGKTVATMFTLMVIGLIATGIS
                     LSQMVPTFEPATLQRAFLIVAASALTMGLIGLIKLEPRFDHSAQAPKADTYTVKQMMA
                     AITENPVAKIFFVYLLLLLAAILSQDVLLEPFGAQAFGMTLEQTSRIVSITNSFTLIA
                     FIIAGFLDGRVKKKYVAQTGNLAALFGFIVIVISGLIANKNAFYVGITLLGFGTGIST
                     IANLSLMFDLTVPEKVGLYIGAWGFSNGLSRLVGLLMAGVIADVATKITGDALHGYLV
                     VFGLEALMLFIAAIMLYRIDVGVFQKKAHEPGFAEKIALASE"
     gene            3518..4147
                     /locus_tag="PLT08_04385"
     CDS             3518..4147
                     /locus_tag="PLT08_04385"
                     /inference="COORDINATES: protein motif:HMM:NF024139.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HNE03731.1"
                     /translation="MTNDEWLSLAGAADLLGVHPSTVRLWSDKGLLPVHKTQGGHRRY
                     KRGEILLWAESNEKSKEEALEPEGMMQEVIKNVRMQISEGRLQEESWYQKLDEDARAQ
                     YRMSSRSLFQGLMIYVATNGREADSEAYAIGYEYASRARRYSLSYVDAAKAFLFFRDT
                     LVASVIKVYNEANVPAKRATEMYARMHTFTDEILISLLETYRKLETANH"
     gene            complement(4176..>5419)
                     /locus_tag="PLT08_04390"
     CDS             complement(4176..>5419)
                     /locus_tag="PLT08_04390"
                     /inference="COORDINATES: protein motif:HMM:NF020926.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1998 domain-containing protein"
                     /protein_id="HNE03732.1"
                     /translation="HLLILLEHLRCAMFELPFQRGEGFGSISDELVDEYLEFLVSNNE
                     AHFSNEKYFWMQDAYPAANISLRSASPQSVVLHTTADDGRPTIVGTVDGESANWMVHP
                     GAIYMHEAQQYFVQELDIENHIAHLVPVGLDYYTEAQQQSEIQITSLNEQIATRGCEK
                     GFGEIQVTTQVVGFRKIRWFTNENLGQEPLDLPPSELQTTGYWIVLSEAALKVLRDAG
                     LWTNDPNDYGPDWQKIRLAVRKRDQFKCQVCGAAETNREHDVHHKTPFRAFIQNGVLD
                     REQANRLENLTTLCQSCHRKVEQNVRIRSGLTGLGFVLGNLAPLFLMCDAGDLGVFSE
                     PAWSAVNGQPAVVLYDLVPAGIGFSEKLFELHDELIRRAHELVSECACEDGCPSCVGP
                     GGENGYGGKAETLAILQQLLK"
BASE COUNT         1337 a         1403 c         1296 g         1383 t
ORIGIN      
        1 ttggcgacac cgcacggagt ggtattacaa cagccagcct tcagcaaata ttatttgcac
       61 ttgggtgaga tctcttccta cccgccgggc tacaaggaga acgcgggtat tttctgccat
      121 accaatccat ggatcatgat cgcagaggca aaagttgggc gcggagataa agcgcttgac
      181 tattacctac gcatcaaccc atcggcgcgg gaaggcatag gggatttgca tcgctgtgag
      241 ccgtacgtct atgctcaaat gatcgcgggc aaagatgctg cgactcacgg cgaggcaaag
      301 aattcctggc tcactggcac tgccgcttgg aattatgtcg ccatcacgca atggattctt
      361 ggaatccgcc cgacgtatga agggttgcaa gtctcgccgg ttgttccggt tgggtggaaa
      421 tcgttcgaag cggtgagaga atttagaagc gtacggtaca acatcaaggt cgaaagaagc
      481 ggtgctggga atcaggtaaa gctggaagtg gatggaaaag ccatcgcagg aaatatcatc
      541 cccttggctg cgaatggaac caaagaagtt cgtgtgcgtg tgatattgag ctagtgattt
      601 tcttgataaa aaaacacatc ccgaaatcgg gatgtgtttt tttatttaga cgccaggctt
      661 gttggcactt tatttccttc gcccttcatg gtcacttcca cccaaagaga agcggcgagt
      721 tcgtagccta tgagatggtc atgcggtttc atttgtaatc gtaacggacc tttgaacggg
      781 gcgcaactaa acaagtgaaa ttgcttgccg ggggtaagac ctaattcgcc aatgtaattt
      841 aatttttcaa ggtcatgggt gcggacgcgg ctaatgacgc aatcagaacc agaggggacg
      901 tcgatcaatg gcatatcttt gacaacaggc atgaagccat ctttggaagg aatcggctcg
      961 ccatgcgggc aacgtgtggg gcgcccggca agttcatcca tgcggttttc gatctcgtcg
     1021 ttgacgcctt tttcgaaggt gtcagaaagt tcgtgggcag ttgcccagtc gtacttcatc
     1081 actttgacga ggaaaacctc agtgaggcga tgcctacgca gggaaggcat ggcgatcttt
     1141 tcgccagttt cagtaaggcg gactccgcga tagggttcat gtacaagata acccagcttt
     1201 tgcaaacgct tgaccatgtt ggaaatggct tgtgcagaag catccacatg cgaacttaaa
     1261 agcgaaagcg gcacctgctc atggtcttgt tgcaggcggt agatctccgc ggcataacgc
     1321 tgcatagcca aacttacaga cattctttaa aacactccaa aatcttcaat tgggttgaaa
     1381 actggcttca actggatata gataaatttt ataacaatta tctagattat tcgtaccgcc
     1441 gtgattatac tacgcgccac ttatcaaaca aagtatggtc aacactacat tttaggagaa
     1501 tatttcatgt tccaaaccaa tatcagcctc agccccgaaa tgaccgccaa cgagcgaaac
     1561 caagcgattc gtgaagcgta taaccaagac cgctctcaaa tgacactcat gcttgcccac
     1621 cagttacgca tccccgaagt ggacattctg cgagcgctcg aaggcgacac cgcccgtgag
     1681 cttgattttg cgcgttggga ggagatcatc cgcgcatttg aggcgttggg cgaggtgacc
     1741 gtcatcgtct cgaacggctc gaccaccatc gaatcgttcg gtcagttcgg cggtttttcc
     1801 aatgctcaag gctttctcaa cgtccgcagc aaatcactgg atatgcacat tcgtggttgg
     1861 gagttggcat cggtcttcgc tttccgcaaa ccgagccacc tcgacaagca cgaatcgctt
     1921 tccttccaat tcttcgataa acgcggcaac tctgccttca aagtctttct caatttcggc
     1981 ggcaaagacc ctgcctctga gttggttgaa gcctataacg acttgatcgg gcgtttttca
     2041 aaataattct tcctcagaaa actctagtga aattctacat aaatctatat actcctaagg
     2101 aaacttgagc ctacaatatc aaccaagagg tgagggcgtc caaatcgccc ttccttttgt
     2161 ttaatcctca acctcaggaa atgctatgtt catcaaaaga cttcaacttg ccctcatcca
     2221 taccgctgtt gccatgacgc ttgtgcccat caacagcacc ctcaaccggg tcatgatctt
     2281 cgatctaggc gtctcaaaaa ccctttttac actgcttgcc atctttccct atttgctttc
     2341 ccccattcaa gttgccattg gctccttctc agaccgtcac cccattctag ggtatcgccg
     2401 cgcgccatac attttcgctg gtctgctttt gtgtgtgatt ggggttgccg tctcaccgca
     2461 gatcgccatc ctaattagca acaactttac cgttggggtg gctgctggca ttctcgcctt
     2521 tggtttatgg ggcatgggct ataacctctc tgctgtttca tatctttcgc tggcatctga
     2581 actttccggc gaaaaagaac gcggtaagac cgtagccacc atgttcacac tcatggtcat
     2641 cgggttgatc gcgacgggca tcagcctgag tcagatggtg cccacctttg aacctgccac
     2701 ccttcagcgc gccttcctga tcgtcgccgc ctcagccctg accatgggac tgatcggtct
     2761 catcaagctg gaaccacgct tcgaccactc tgcccaagcc cccaaagcgg atacgtacac
     2821 cgtcaagcaa atgatggctg ccattactga aaaccccgta gcgaaaatat ttttcgtata
     2881 cctgctcttg ctcctcgccg ccatcttgag tcaggatgtt ctgctcgaac cctttggtgc
     2941 tcaagccttc ggcatgaccc tcgaacagac ctcgcgcatc gtctccatta ctaattcgtt
     3001 cacactgatc gccttcatca ttgcaggttt tctcgatggg cgcgtgaaaa agaagtacgt
     3061 tgcacaaacc ggcaatctcg ccgcgttgtt cggcttcatt gtcatcgtca ttagcgggtt
     3121 gatcgccaac aaaaatgcct tttatgttgg cattaccctg ctcgggtttg gcaccggaat
     3181 ttccaccatc gccaacctct ccctcatgtt tgacctgacc gtccccgaaa aggttggttt
     3241 gtatattggc gcgtggggct tctcgaacgg actctcgcgt ttagttggtc tactcatggc
     3301 aggtgtcatc gcagacgtag caaccaaaat cactggtgat gcccttcatg gctatctggt
     3361 ggtctttggg ttagaggcat tgatgttgtt catcgctgcg atcatgttgt accgcattga
     3421 tgtgggcgtt ttccaaaaga aggcgcatga acctgggttt gccgaaaaga tcgcgcttgc
     3481 ctcagaatag tttgaaaaag caggatatcg gctgaatatg acaaacgatg aatggctttc
     3541 tctcgctggt gccgccgatc tgctgggggt tcacccgagc acggtgcgat tatggtctga
     3601 taaaggctta ctccccgttc ataagactca gggtgggcat cgccgctata agcgtggaga
     3661 aattctcctt tgggcagaat caaatgaaaa gtccaaagaa gaggcgcttg aacccgaagg
     3721 catgatgcaa gaagtcatca agaacgtccg catgcaaatc tctgaaggac gacttcaaga
     3781 agaatcctgg tatcaaaaat tggatgaaga tgcccgtgca caatatcgca tgagttcgcg
     3841 ttcgttgttt caagggttga tgatctatgt tgcgaccaat gggcgtgaag cagactcgga
     3901 agcctatgct attgggtatg agtatgcctc gcgggcgcgg cgttacagcc tcagttatgt
     3961 agatgctgcc aaagcatttc ttttctttcg agataccttg gtagcttcgg tcatcaaggt
     4021 atataacgag gccaatgttc cggcaaagcg cgccacagaa atgtacgcac gtatgcacac
     4081 cttcaccgat gaaattctca tcagcttgtt ggaaacctat cgcaagctgg aaacggcaaa
     4141 tcactaaaaa ggacgagcgt aggctcgtcc tttttttatt tcaataactg ttgcaagatc
     4201 gccagcgttt ctgccttgcc cccataccca ttctcgccgc cgggtcctac gcatgatggg
     4261 caaccatctt cgcaggcaca ttcactcacc aattcatgcg ctcgtcgaat cagttcatca
     4321 tgcagttcaa ataatttttc gctgaagcca atccccgccg gaacaaggtc gtacaacacc
     4381 actgctggtt gaccattcac cgcagaccaa gccggttcag aaaagactcc caagtcaccg
     4441 gcatcacaca tcaaaaataa cggcgcgaga ttaccgagca caaatcccaa acccgttaag
     4501 ccactgcgaa tacgcacatt ctgttccact ttgcggtgac agctctggca aagcgtggtg
     4561 aggttttcaa gccggttcgc ctgttcacgg tctaagactc cgttttgaat gaaggcacgg
     4621 aagggagttt tgtgatgcac atcatgctcg cgattcgtct ccgccgcgcc gcacacctga
     4681 catttgaact ggtcccgttt tctgacggcg agtcttatct tttgccaatc gggtccgtag
     4741 tcgttcgggt cattggtcca tagaccagca tcacgcaaca ctttaagcgc tgcttctgag
     4801 aggacgatcc aatagccggt cgtctgtaat tcagaaggcg gaaggtcgag cggttcctgc
     4861 ccaagattct cattcgtgaa ccagcggatc ttgcgaaagc ccacgacttg tgttgtgact
     4921 tgaatctcac cgaagccctt ttcacagccg cgtgtagcga tttgttcatt caacgatgtg
     4981 atctgaattt ccgattgctg ttgagcttca gtatagtaat ccaacccaac tggcacaaga
     5041 tgagcaatat gattttcaat atcaagttcc tgaacgaagt attgttgggc ttcgtgcata
     5101 tagatcgcgc cgggatgaac catccaattc gcagactcac catccacagt tcctacgatc
     5161 gttggtcgtc catcatctgc ggtggtatgc aaaaccacgc tttgtggaga tgccgaacgc
     5221 agagaaatat ttgccgcagg ataggcatct tgcatccaaa aatatttttc gttcgagaaa
     5281 tgcgcttcat tgtttgaaac aagaaactca agatactcat ctaccagttc gtctgaaatg
     5341 gagccaaagc cttcgccgcg ctgaaacggc agttcgaaca tagcacagcg caagtgctca
     5401 agcagaatca acaaatgat
//
LOCUS       DAOQJE010000114         5415 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_8746_length_5415_cov_7.445336, whole genome shotgun sequence.
ACCESSION   DAOQJE010000114 DAOQJE010000000
VERSION     DAOQJE010000114.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 5415)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5415)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5415
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8746_length_5415_cov_7.445336"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            140..>2011
                     /locus_tag="PLT08_04395"
     CDS             140..>2011
                     /locus_tag="PLT08_04395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE03733.1"
                     /translation="MTKLKNHILDGVISAIAGFVISFALAQRFFDLTSLPLIDQLFLV
                     FIPAVAVFICLLFLLPLLKKALEGIYTPACVFLVLWAALSSLLTLFVVHESLLFFLFL
                     FFVFLLFVIPSARSIQSLIEASGRRRVFGAWLFAFIASSLLIGFLDNFYFTTVEVILL
                     TVVLQAVFGVGGYFLMGRVRRVANERWFDALIHGVLFVLLIGFIIWLFQTSRMVSLFP
                     ISYFVLNDETSGLFLFVSLLALPWQAWFHLKLKFSGFYNRAKQTKIYSYVSANLAGFS
                     LALVFFILYLIFASVINDPRFDVDDIFFDADGLNYRIRLTTDTWHDFYWRSVHPFMIL
                     LFKPPVDLISFFLKGNKLWGAYVFVALGGAACVYLAWMFIKSATGNSVYASLIAAMLG
                     LTASHLIFGSLLESYIFLAASLLLFYILLLKDKPLPPLIFASLMTIGITYTNFAQNVI
                     ALFAVRLNIKQMFRFVVSVLVFLVLLTLLNNVLYPDSNPLFFIPSTLQAEQANFFPLN
                     SLRIVGLIRGFAFHNIIAPTPIFYDKDIPFIQFRFFKPEINEISRYDLPIQKITVWFW
                     LALLIVGAILFLITIKKNQHLRLSLGLVGGILLNVMLHLRYGKELFLYSQNWTYALVL
                     "
     assembly_gap    2012..2111
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2112..3560
                     /locus_tag="PLT08_04400"
     CDS             <2112..3560
                     /locus_tag="PLT08_04400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE03734.1"
                     /translation="DAIFSAVIALAVFAFFIGLLNMASRFPNEFDILSYVLPSTTLLF
                     FCVGILAALPASIGLSALARIHNVEQTRLYKFVDANLGGIVIALLFFAIYLMLATIFN
                     QPSFDFDDIYFDTDSALYRYRFGTEFYKDYYDRTVHPYVLIIVRPFVWLVSLLLKGDM
                     LYGAFIVTALTGALCVFLVWYFVREYTSNSLYALLIAGLFGASTSQLAFGSLIENYIF
                     LGAAALIFMVLLLKDQPMYLLIIAGLTSFGITISNIAQTVIAHFFVKRNIPQLIKYGL
                     IVGAFIIPLNLLNNFIYPDAHPYLWEVSTLQFEEKNVFQPTILRANYLSRVMVMHSFV
                     APDPLFIKEDIPFIKLWLFRAAQKQDLLEIARYETALSKWLVTVWLLFVAVGGVLFLK
                     NLFKQDNRFPFAFIFIVLFSFVLHMRYGRDVFLYSTNWTYAITLFLALAWREIANKRW
                     FQIPLFAFIAFQMINNSRLIFFMLISAYLHLR"
     gene            3575..5239
                     /locus_tag="PLT08_04405"
     CDS             3575..5239
                     /locus_tag="PLT08_04405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE03735.1"
                     /translation="MQSFDKRLPLAWLLGIPLSYFIVSYFENFYKTGFQIALFTIIVH
                     SLVNLFLYYLFGRAIKEFLVNRISAVFVTILFTGVAIFIPALYNMAKPYQNLFDVSAF
                     HLDASLRISFFNALIPAFFIAMVVLHVAKQRKWDETRFFKFIDENLEGLLIAVLFFSA
                     YLIFASIFNRPSFNEDDIFFDADSRLYRWRYATENYRDYYWRPAHPFILIIVRPLVGI
                     LAFLFNGDTLFAAFTLNALTAALCVFLVWYFVKHSVGYRLYALLIATLFGASATQLVF
                     GSIIESYIYLSAVALIFLVLLLKDSPLYLQVIAGLVAFGITISNVGQTFIAHFFIKRN
                     IKQIIIYGVMIGLFVVPLTLLNNYIYPDSQPYFWDLSLLDGEGHNQFPATLQRTNYLA
                     RVMALHSFVAPEPLTIDDEFAFTKVWMFRASIKKDPMRLANYETPLGDGLVIAWAGLL
                     ALGGVLFLKNIIKKYDGYSIAFLVTLLFYFLLHMRYGKDVFLYATNWTYAITLFLALA
                     WRDLAGKRWFQVILLAFVLLLLVNNAGLLKTMLEVTSPAIQTPIWR"
BASE COUNT         1161 a         1186 c         1071 g         1897 t
ORIGIN      
        1 atgccgtctc gttcgataac gtggacattt ggatggataa tgaagattgg tctgagttca
       61 agcatttgct gaacgcatta gggaagtaat taattaggtg gcagtcacat agaatgactg
      121 ccacctttta atttttgtca tgacaaaact aaaaaaccat atcctagacg gcgtgataag
      181 cgccatagct gggttcgtta tttcctttgc gctcgcgcag agattttttg atttgacttc
      241 cctgccattg atagaccaat tatttttagt gtttattccg gctgtcgcgg tatttatttg
      301 tcttttattt ttgttgccac tcctgaaaaa agcacttgaa ggaatatata cacctgcgtg
      361 tgtatttctt gttctttggg cagccctttc atcgctcctt actttgtttg tggttcacga
      421 gtctttgctc tttttcctct ttctcttttt tgtttttctc ctctttgtaa ttccctctgc
      481 acgatctatt caaagcctaa tcgaagcaag cgggcgtcgc cgtgtatttg gagcatggtt
      541 attcgctttt attgccagct cactgctcat tggcttttta gataatttct atttcaccac
      601 tgttgaagtt attttgttga ctgtggtttt acaggcagtt ttcggtgtgg gtggatattt
      661 cctgatgggc agagttcgac gcgtagccaa tgagcgttgg tttgatgcgt taatacatgg
      721 cgttttattt gttttgctta ttggctttat tatctggctt tttcaaacga gccgaatggt
      781 ttcgcttttc cctatctcct actttgtgct gaacgatgaa accagtgggc ttttcttgtt
      841 tgtcagtctg ctagctttgc catggcaagc ttggttccac ctcaaactga agttcagtgg
      901 cttttacaat cgcgccaagc agacaaaaat atattcttac gtaagtgcta acttggctgg
      961 gttttcatta gctcttgtgt tttttatctt gtatttgatt ttcgcttcgg tgatcaatga
     1021 tccacgtttt gatgtggatg atattttctt cgacgccgat ggattgaact accgcatccg
     1081 cctcaccacc gatacatggc acgatttcta ttggcgttcg gttcacccat tcatgatttt
     1141 gctcttcaaa ccgcccgtag atttgatttc atttttcctc aagggtaaca aactatgggg
     1201 tgcatatgtc ttcgtagcat tgggcggcgc agcatgtgtg tatcttgcat ggatgttcat
     1261 caagtctgcg acggggaatt cggtctatgc gtcactcatc gccgccatgt tgggattaac
     1321 cgcctcgcac ttgatttttg gctcgttgct cgaatcttac attttcctcg ctgcgagttt
     1381 gttgctgttc tacatcttgc tcttgaagga taaaccttta ccaccactga ttttcgctag
     1441 tttaatgaca attggtatca catatacaaa ctttgctcag aatgtgattg cgttatttgc
     1501 agtgcgcctg aacattaagc agatgttccg ctttgtggtt tcagtgcttg tatttctggt
     1561 tttgcttacc ctgcttaaca atgtactcta tccagattcg aatccgcttt tctttatccc
     1621 atccacatta caggctgagc aagcgaattt cttcccttta aactcattac gtatagttgg
     1681 gctgataaga ggttttgcat ttcataacat tattgccccc actccgattt tttatgataa
     1741 agatattccg tttattcaat ttcgtttctt caaaccagag attaacgaaa taagccgata
     1801 tgaccttcca attcaaaaga tcacagtttg gttttggctg gcactgttga tagttggtgc
     1861 cattctcttt ctgataacaa taaagaagaa tcagcatttg cgcttatctt taggtttagt
     1921 aggtggaata ttgctgaatg tcatgttgca cttgcgatat ggcaaggaat tgttcttata
     1981 ttcccaaaat tggacctatg ccctagtttt gnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2041 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2101 nnnnnnnnnn ngatgcaatc ttttctgccg tcatagctct tgctgtgttc gcctttttta
     2161 ttggattgct caacatggca agtcgcttcc ccaatgaatt tgatattctg tcctatgttt
     2221 tgccatcaac aacattgttg ttcttttgtg ttgggatttt agctgcttta cctgcttcaa
     2281 tcggtttatc agctcttgca agaatacata atgttgagca aactcgcctt tataaatttg
     2341 tagacgcaaa cctgggtgga attgtgattg ccttgctgtt ctttgcgatt tatttaatgc
     2401 ttgcaactat tttcaaccaa cccagctttg actttgacga tatttacttt gatactgata
     2461 gtgcactcta tcggtatcgt tttggtacag aattttataa agattattat gaccgcactg
     2521 ttcaccccta tgtgctaatc atcgttcgac cattcgtctg gttggtatcc ctccttctca
     2581 agggcgatat gctttatggc gcattcatcg ttacagcatt gaccggcgct ctgtgtgtct
     2641 ttctcgtttg gtattttgta agggaataca cctcaaactc actgtacgct ctgttgattg
     2701 caggcttgtt cggcgcttct acatctcaac tcgcgtttgg ttctctaatc gagaactaca
     2761 tcttcctcgg agcggctgct ctgatcttca tggtcttgct tttgaaagac cagccgatgt
     2821 atttgctcat aatcgccggg ttgacctctt ttggcattac tatctccaat atcgcacaga
     2881 cggtgattgc gcatttcttt gtcaagcgta atattccgca actcataaag tatggactga
     2941 ttgtaggtgc tttcatcatt ccattgaatc tgcttaataa ctttatttac cccgatgcac
     3001 atccctactt atgggaagtg tcaactctgc agtttgaaga aaaaaatgta tttcaaccaa
     3061 caatcctccg cgctaattac ttgagccgtg tgatggtgat gcacagcttt gtcgcgccag
     3121 acccattatt tataaaagaa gatattccat ttatcaaact ttggctattc cgtgctgcgc
     3181 aaaagcaaga ccttctcgaa attgcccgat acgaaacagc actgagtaaa tggcttgtca
     3241 cggtttggct tttgtttgta gccgtaggtg gtgtcctttt cctcaaaaat cttttcaagc
     3301 aggataatcg tttcccattt gcttttattt ttattgtctt gttctctttt gtgcttcata
     3361 tgcgttacgg aagagacgtg ttcctgtact ccacaaactg gacctatgcc atcaccctgt
     3421 tccttgcctt ggcgtggcgt gaaattgcca acaagcgctg gttccagatt ccgcttttcg
     3481 ccttcattgc ttttcaaatg atcaacaatt cacgtttgat tttctttatg ctgatttcag
     3541 catatctcca cctccgctaa tcaaggattc tcatatgcag tcattcgata aacgcctccc
     3601 tttggcttgg ttgctaggga ttccactttc gtatttcatt gtttcgtatt ttgagaattt
     3661 ctataaaact ggctttcaga ttgctctatt cactatcatc gttcacagtc tggtcaattt
     3721 gtttttgtat tatctttttg gtagagcaat caaagaattt cttgtaaacc gcatcagtgc
     3781 ggtttttgta actattctat ttactggtgt ggcgattttt attccagctt tatataacat
     3841 ggcaaagccg taccagaatt tgtttgatgt aagtgctttt catctggatg caagccttcg
     3901 tatctcgttt ttcaatgctt tgattcctgc tttttttatc gcgatggtcg ttttgcatgt
     3961 tgccaagcaa agaaaatggg acgagacgcg cttcttcaaa ttcatagacg aaaatttaga
     4021 aggcttgctg attgccgttc ttttcttttc tgcttacctc atcttcgcat ctatcttcaa
     4081 tcgtccatcc ttcaacgagg atgatatttt ctttgacgca gatagccgtt tgtatcgttg
     4141 gcgttatgcc acagaaaact atcgcgacta ttattggcgt cctgctcatc cattcatcct
     4201 tattatcgtt cgcccgttgg ttgggattct tgctttcctt ttcaatgggg ataccctctt
     4261 cgcggctttt accctcaatg cgttgaccgc cgccttgtgc gttttcttgg tctggtattt
     4321 tgttaaacac tctgtcggat atcgtctcta cgcacttttg atcgcaaccc tttttggcgc
     4381 ctctgcgact caacttgtgt ttggttctat tatcgagagc tatatctacc tttcagctgt
     4441 tgcgttgatc ttccttgtcc tgttgctaaa ggatagccct ttatatctac aagtcattgc
     4501 tgggttggta gcgttcggta tcactatctc taacgtcggg cagacgttca ttgcgcactt
     4561 cttcatcaaa cgcaatataa aacagatcat tatttatgga gtgatgattg gtttgtttgt
     4621 cgttcctctc accttgttga ataactatat ctatcctgac tctcagccgt acttttggga
     4681 tttatcactg ctcgacggtg aaggacataa tcaatttccc gccacccttc agcggacaaa
     4741 ctatcttgca cgtgtcatgg cattgcatag ctttgtcgcc cctgaaccat tgaccattga
     4801 cgatgaattt gccttcacca aagtgtggat gttccgtgcc tcgatcaaaa aagaccccat
     4861 gcgcctcgcc aattatgaga cgccgcttgg tgatggtttg gtcattgcat gggctgggtt
     4921 attggctttg ggtggggtgc ttttcctgaa aaatatcatc aaaaaatatg atggatattc
     4981 catcgcgttt cttgtcaccc tgctctttta ttttttactt cacatgcgtt atggcaaaga
     5041 tgttttcctc tatgccacca actggaccta tgccattacc ctcttcctcg ccctcgcctg
     5101 gcgtgatttg gcagggaagc gttggtttca ggtcattcta ctagcctttg ttttgctgct
     5161 gttggtgaac aatgctggtc tgctaaagac aatgctcgaa gtgacttccc ccgcaatcca
     5221 aaccccaatt tggcgctaaa aataccaata tgtaataaaa aagccctgct tttgcagagc
     5281 ttttttgtgg gcgctgaagg actcgcctgt ccctgtaagg ggaacccccg acaaaaagaa
     5341 aaaagcccta cttttgcagg gcttttttgt gggcgctgag ggactcgaac ccccgacacc
     5401 ttcggtgtaa acgaa
//
LOCUS       DAOQJE010000115         5391 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_8811_length_5391_cov_4.987256, whole genome shotgun sequence.
ACCESSION   DAOQJE010000115 DAOQJE010000000
VERSION     DAOQJE010000115.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 5391)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5391)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5391
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8811_length_5391_cov_4.987256"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..872
                     /locus_tag="PLT08_04410"
     CDS             <1..872
                     /locus_tag="PLT08_04410"
                     /inference="COORDINATES: protein motif:HMM:NF014893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HNE03736.1"
                     /translation="LAGYPVETDLSEALKKHKPDAVIVSNPTSIHLDIAIPAAEAGCA
                     ILLEKPIAGSMERVDVLQKTVKKSGSKVLVAFQFRFHPGMVKTRELIENGEIGRVVSA
                     SVHFGEYLPAWHPWEDYRQGYAARADMGGGVVATQCHSLDYLPWLVGKKVESVWGFAD
                     KLSDLEVTADDTAKIGLRFEGGAVGSLHLDYNQQPPEHEFRIIGTNGTIKWNLVDGAA
                     RIYRTEKKDWDVYPLPEGWERNVMFQQQTKHFVDIVNGKAQPSCTLEDGVQVQRIIAS
                     VHESQKTGKLIKL"
     gene            1111..2838
                     /locus_tag="PLT08_04415"
     CDS             1111..2838
                     /locus_tag="PLT08_04415"
                     /inference="COORDINATES: protein motif:HMM:NF004040.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional sulfate
                     adenylyltransferase/adenylylsulfate kinase"
                     /protein_id="HNE03737.1"
                     /translation="MAKANLITPYGGKLVDLVVKGAERDALIARAGHLPSIKITMRNL
                     CDLELIATGGFSPLTTFMGKADYERVLKEMRLADGTLFPLPITLTADPKELPTVGEEL
                     ALRNANFDLIAVMRLDEVYHWDANTEAALAYGSTDTKHPMVSEMGRWGKVCISGPLQV
                     INLPKYYDFVNLRHTPAQVREMLEAMGNDNVVAFQTRNPLHRIHEELTKRAAAQVNGS
                     LIVHPVVGMTKPGDVDHYTRVRTYKALVDNHYDQKSTMLSLLPLAMRMAGPKEALLHA
                     IIRRNHGANHFIVGRDHAGPGNDSTGKPFYGPYDAQELMTKHEAELGVKMVPFEMLVY
                     LPDEERYVEEKDVPKGAKVANISGTQVRDDYLAKGKLLPEWFTRPETAEILREMYPPR
                     HKQGFGIWFTGLSGSGKSATTAVLTTLLLERGRETAILDGDVVRTHLSKGLGFSKEDR
                     DTNILRIGFVAGEIVHAGGIVICAAISPYRATREEARKMVGENFIEVYMDTPVEVCEQ
                     RDVKGLYAKARQAMADGKPMGFTGVDDPYEPPVKPEITLKGYESTPEDNARAIIKYLE
                     EQGFLLPVK"
     gene            2887..4437
                     /locus_tag="PLT08_04420"
     CDS             2887..4437
                     /locus_tag="PLT08_04420"
                     /inference="COORDINATES: protein motif:HMM:NF014425.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000030 - mannosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006493 - protein O-linked glycosylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid carrier-dependent
                     glycosyltransferase"
                     /protein_id="HNE03738.1"
                     /translation="MTKSHSASFLILGLLALSGTFLILYATPQGMGLFDDSIAYIAGA
                     RSIVAGQGYREAWLASNQPVTHFPPGFSTVLALVGISGLDPLRGTRLVNAVVFGANIF
                     LLGLIGWRMTKSKLAGMALSALLLLSGELFRVHTTAMSEPLYIFFTLAAFLAFSQTFV
                     SEEKQTNKFLLLASALTAFAYLTRYAGLALFATFLVSLFLLHPTWKQRLTSAGIFLAG
                     FVPFALAWAVRNRLLTDNATNRKLVYHPITPENIQLGISNFASFILPIEEWRKVLIKV
                     PNLVAVILIALILALLGWVVLKGLKKFFNPASPTPEVLSFVSTLYVFGYLASILSSMT
                     LFDASTKFQLRILAPVYVSLLIMLVYFGYWLWQKQKIALILLSLVAFSFSVSSFTSTL
                     TQLRKGGQGYASFQWFDSEAMKFMQNLPAEARIYSNETAAVYLYTDHPGYVLPDLIDP
                     VTGLPRGRFDEGVKALQEDVLSGNAVLALFDVDSQDEDVQSTYQILANGLYLAFDKQG
                     DKIYTAYP"
     gene            4472..5299
                     /locus_tag="PLT08_04425"
     CDS             4472..5299
                     /locus_tag="PLT08_04425"
                     /inference="COORDINATES: protein motif:HMM:NF024950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HNE03739.1"
                     /translation="MTIFDKFSLKDRVAVVTGGGGQLGFEFCKTLAEAGAAVVAADLN
                     MDFAGKTATRLTEAGYAAMAFPLDVTRPESTRELVAETVKQFGRLDILVNSAALDPKF
                     DPTAAEKGIAPGAFEDYPLADWQAALNVNLTGMFLTTQACVKQMVSQGKKGSVINICS
                     TYGLNGPDQRIYIKDGKRVAYKPVYYTTTKAGVMGFTKYLAAYYAETEIRVNALTPGG
                     VYNNHEEYFVKNYSAKTILGRMAKKDEMNGALLFLASDASSYMTGNNVIVDGGWTAW"
BASE COUNT         1253 a         1343 c         1372 g         1423 t
ORIGIN      
        1 aactggcagg ctatcccgtt gagacagacc tgtctgaagc cttgaaaaag cataaaccag
       61 atgcggtcat cgtctcgaac cccacttcaa tacatttgga tattgccatt cctgccgcgg
      121 aggcgggctg tgctattttg ctggagaaac ccattgctgg ctcaatggag cgcgtggatg
      181 ttttgcaaaa gactgtgaaa aaaagcggct cgaaggtgtt ggtggcgttt cagttccgct
      241 ttcacccggg aatggttaag acccgtgaac tgattgaaaa cggtgagatt gggcgggttg
      301 tctcggcaag cgtgcatttc ggtgagtatt tgccagcttg gcacccgtgg gaagattatc
      361 gtcagggata tgcggcacgg gcggatatgg gcggaggcgt ggtggcaaca caatgtcact
      421 cactcgatta tttgccgtgg ttggttggaa agaaggtgga gtctgtttgg ggttttgctg
      481 acaagcttag cgaccttgaa gtgactgcgg atgacacagc caagatcggg ctgcgatttg
      541 aaggcggcgc agtaggaagc cttcacctag actataacca acagccgcca gagcatgaat
      601 tccgcattat tggcacgaat ggaaccatta agtggaattt ggtggatgga gcggcgagaa
      661 tttaccgcac agagaaaaaa gattgggatg tgtacccgct accggaaggg tgggagcgga
      721 acgtcatgtt ccaacagcaa acgaaacatt ttgtggatat agtcaacggc aaggcacagc
      781 cttcttgtac acttgaagat ggggtccaag tgcagagaat catcgcttcg gtgcatgagt
      841 ctcagaagac agggaagttg ataaagttat aggtgagaaa gcggcagaga acctgtcgct
      901 tttttgttgg attcgctttc agggatggga aagtttggaa ggcgtggcag gctcgaggtc
      961 cagaaagagg cggtgggttt ccaaaattca cccactgaat tttggagtat aattggcgct
     1021 tgtgcaaact ggtatgggcg atggatttcc atgcatcccc atactttatg cccacgggca
     1081 ttatctaaaa caaatggagt aacaatcaca atggctaaag caaatcttat taccccttat
     1141 ggtggaaaat tagtggactt ggtcgtgaag ggtgccgaac gcgatgcatt aattgcccgt
     1201 gccgggcatc tgccttccat caagatcact atgcgtaact tgtgcgacct tgaattgatc
     1261 gctacgggtg gtttttcgcc tttgacgact ttcatgggca aagcggacta cgaacgtgtg
     1321 ctcaaagaaa tgcgccttgc agatggaacg ctctttccgc tgccgattac attgactgcc
     1381 gacccgaaag aattgccgac cgtgggtgaa gaactcgcgc ttcgcaatgc aaactttgac
     1441 ttgatcgctg tgatgcgcct cgatgaggtc tatcactggg atgcgaatac cgaagcggct
     1501 ttggcatacg gctcgaccga taccaagcac ccgatggttt ctgaaatggg acgttgggga
     1561 aaagtgtgta tctctggtcc tttgcaggtg atcaaccttc ccaagtatta tgactttgta
     1621 aatctgcgcc ataccccggc tcaagtgcgt gaaatgcttg aagcaatggg taacgataat
     1681 gtggtcgctt tccaaacccg caatccttta caccgcatcc atgaagaatt gaccaaacgc
     1741 gctgcggctc aggttaacgg ttctttgatc gttcaccctg tggtgggtat gaccaagcct
     1801 ggcgatgtgg atcactacac ccgcgtccgc acgtacaagg cattggttga caatcactac
     1861 gatcagaaga gcaccatgct tagcttgttg ccgctcgcca tgcgcatggc gggtccaaag
     1921 gaagcgttac tgcatgccat tatccgccgc aatcatggcg cgaatcactt catcgttggg
     1981 cgcgatcatg cgggccctgg caatgactcg acgggcaaac cgttctacgg tccttacgat
     2041 gcgcaggaat tgatgaccaa acatgaagcg gaactcggcg tgaagatggt tccgttcgaa
     2101 atgcttgtgt atctccctga tgaagaacgc tatgtggaag agaaagatgt tcccaagggt
     2161 gccaaagttg ccaacatctc tggcacacag gtgcgtgatg attatcttgc caagggcaaa
     2221 ctgctccccg agtggttcac ccgccctgaa accgctgaaa ttttgcgcga aatgtatcct
     2281 ccgcgccaca agcagggctt cggcatttgg ttcactggtt tgagcggctc tggcaaatct
     2341 gccaccaccg ctgtcctcac caccctgttg ctcgaacgcg gacgtgagac cgccatcctt
     2401 gatggagatg tagttcgtac ccatctgtcc aagggacttg gttttagcaa ggaagaccgc
     2461 gataccaaca ttctccgcat tggctttgtg gctggcgaga tcgtgcacgc cggtggcatc
     2521 gtcatctgcg ccgctatcag tccgtaccgc gccacccgcg aagaagctcg caagatggtc
     2581 ggtgaaaact tcatcgaagt ttacatggac actcccgttg aagtttgtga acagcgcgat
     2641 gtaaaaggtt tgtatgccaa ggctcgtcag gcgatggcag atggcaagcc gatgggcttc
     2701 acgggcgttg atgatccgta tgaaccgcct gtcaagcccg agatcacact caaaggctat
     2761 gaatccacgc ccgaagacaa tgcgcgtgcc atcatcaagt atttggaaga acaaggcttc
     2821 ctgcttccgg tgaagtaatt gctattcagg tgacagtcac cattatggtg actgtcacct
     2881 cgttgtatga ccaaatccca ctccgcttca ttcttaatcc tcggtctgct tgctctttca
     2941 gggacctttc tcatcctgta tgcgactcca caaggcatgg gactcttcga tgattccatt
     3001 gcttatattg ccggtgcgag aagcattgta gcggggcaag gctatcgcga agcgtggctt
     3061 gcttctaatc aaccagttac acacttccct ccaggttttt caactgtttt agccctcgtc
     3121 ggcatcagcg gacttgaccc tttgcgtggc acacgcttgg tcaatgctgt tgtgtttggc
     3181 gcaaatatct ttttgctcgg gctcattggc tggcgcatga ccaagtcgaa gctggctggg
     3241 atggctttgt ctgctttgct tttgttaagc ggggaacttt tccgcgttca taccacggca
     3301 atgagcgagc cgctgtatat cttttttaca ctggcggcat ttctagcctt ctctcaaacg
     3361 tttgtctctg aagagaaaca gacaaacaaa tttttgttac tggcgtctgc tcttaccgct
     3421 tttgcttacc taacccggta tgccggtctt gccctctttg cgacctttct cgtttcactc
     3481 ttccttcttc atcccacttg gaagcaacgc ctaactagcg caggtatttt cctcgcaggc
     3541 tttgttcctt ttgcgcttgc ttgggcggtt cgtaatcgct tactgaccga caatgccacc
     3601 aaccgcaagc tggtctatca cccgatcact cctgaaaata ttcagcttgg catttccaat
     3661 ttcgcctctt ttattttgcc cattgaagaa tggcgtaagg ttctgatcaa agttccaaat
     3721 ctggtcgctg ttattcttat tgctcttatt ttggctctgt tgggttgggt tgtgctcaaa
     3781 gggttgaaaa aattttttaa cccggcttct ccaacccctg aagttctttc ctttgtcagc
     3841 acgctttatg tttttggcta tcttgcatcc atcctttctt cgatgacatt gttcgatgcc
     3901 agcaccaaat tccaactccg catccttgcg ccagtgtacg tcagcctgct cattatgttg
     3961 gtctattttg ggtactggct ttggcaaaaa caaaaaatag ccctcattct cttgtcactg
     4021 gtcgccttct ctttttctgt ttctagtttt acgagtaccc tcacccaact ccgcaaaggc
     4081 gggcagggat atgcttcctt ccagtggttc gactcagaag cgatgaagtt catgcaaaat
     4141 cttcctgcag aggcgcgtat ctattcgaat gaaacggcgg ctgtatatct ttatacggac
     4201 caccctgggt acgtcttgcc cgatctcatt gacccggtca ccggtttgcc gcgtgggcgt
     4261 tttgacgaag gagtcaaagc attgcaagaa gatgttttat ctggaaatgc cgtgctggcg
     4321 ctgttcgatg ttgattcgca ggatgaggat gtacaatcaa cgtatcaaat tctcgccaat
     4381 ggtttgtatc tcgcttttga caaacagggc gataaaatct ataccgcata tccgtaaggc
     4441 gattctatcc cttacacaaa aaggaattca gatgactatt ttcgataaat tcagtttgaa
     4501 agaccgtgtg gctgttgtca ctgggggcgg tgggcaactt ggttttgagt tttgcaagac
     4561 ccttgctgaa gctggagctg ctgtggttgc ggcagacctc aatatggatt ttgctgggaa
     4621 aaccgcgacc cgcctcaccg aggctgggta cgccgccatg gcatttcccc tcgatgtgac
     4681 ccgccccgaa tcgacccgtg agttggtcgc tgaaaccgtc aagcagtttg gtcgccttga
     4741 tattctcgtc aactccgcag ctcttgaccc aaaatttgac cccactgccg ctgaaaaggg
     4801 catcgccccc ggcgcctttg aagattatcc acttgcagac tggcaagccg ccctcaacgt
     4861 caacctgact ggcatgttcc tcactaccca agcctgtgtg aaacagatgg tgtcgcaagg
     4921 taaaaagggc agtgttatca acatctgctc tacctatggc ttgaacggac ctgaccaacg
     4981 tatttacatc aaagacggca aacgggtagc ctataaaccc gtctattaca ccaccaccaa
     5041 agccggtgtg atgggtttta ccaagtacct tgccgcttac tatgctgaga cggagatccg
     5101 cgtcaatgca ttaacccccg gcggtgttta caacaaccac gaagaatatt tcgtcaagaa
     5161 ttattctgcc aaaactatcc ttggacgcat ggcaaagaaa gacgaaatga atggtgcctt
     5221 gctcttcctc gcttcggatg cctcttctta catgaccggc aacaacgtta ttgtcgacgg
     5281 tggttggacg gcgtggtagt gagcgatact cggttattga agatcaaaga tcgaattgcg
     5341 aatatcgaga acttatgacc aatattcttg ccattatccc tgctcgcggc g
//
LOCUS       DAOQJE010000116         5383 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_8830_length_5383_cov_5.787350, whole genome shotgun sequence.
ACCESSION   DAOQJE010000116 DAOQJE010000000
VERSION     DAOQJE010000116.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 5383)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5383)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5383
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8830_length_5383_cov_5.787350"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..842
                     /locus_tag="PLT08_04430"
     CDS             <1..842
                     /locus_tag="PLT08_04430"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HNE03740.1"
                     /translation="EKELKATLQEMAILQNQLADANIKLLELEKGKIATKSTEQAEVL
                     ASISQELRQPLSSIVGYTDLLLGESVGILGALQRKFVDRIKVSTERIRSLTDDMIQIT
                     TLATDLYSLNPEAVDLNSIIDNAMSYTSSQIREKNISIHLEVPKAVTSIHADREALQQ
                     IVIHLLQNAGAATPFEGTIRLKVQTQNEDGAEYILIQVADQGGGIASEDIPRVFTRLY
                     RADNVLIQGVGDTGVGLSIAKTLTEAQHGRIWVESKVGEGSTFSVLLPISGDKLADKQ
                     KRK"
     gene            846..1655
                     /locus_tag="PLT08_04435"
     CDS             846..1655
                     /locus_tag="PLT08_04435"
                     /inference="COORDINATES: protein motif:HMM:NF013630.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HNE03741.1"
                     /translation="MRGVRDFGNALVAAILSIGLTLGALSISLVGFIPEEVATPSPTL
                     IVSPIPVTATNTLPPTATLLVTVNIPTSSPTNTIVPPTSCQPPVGWVGVVVQVGDTLD
                     SLAARYGTYKDILKNKNCLFTDSLIAGTLLYVPGGISTSTVVACNQGAVGWIKNYMVR
                     PGDTFYNIGTRYGVNANLIKLVNCRTSDIIYAGEILYVPNVPTRTPTFTPLPGITYTA
                     APLLTEPLTLTVLPFTATYPPTQTSTATTAPTASPIPTQTASLTAIPTPTP"
     gene            1682..3172
                     /locus_tag="PLT08_04440"
     CDS             1682..3172
                     /locus_tag="PLT08_04440"
                     /inference="COORDINATES: protein motif:HMM:TIGR00350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HNE03742.1"
                     /translation="MKSSNLFVWALCLAVFISACGAAAPTQAPQPFVLITAAPNASPT
                     PTPFQPSLASPTAPFNTSGSNPAPQPILATETTFPTATPQASPTATAPIEQLFPTQAA
                     PPPAEALVNPTALPPLTTENDTINFLLVGSDRRPTGTSHRTDTLLIAVVWYKEGQVSL
                     ISIPRDTWLYIPTVGMQRINTAYQSGEGVYPGGGMGLLKDTILQNFGIRIDHTAMVEF
                     DGFRRIVDTLGGIDVPISCPYTDWRLISPGLDENDENNWWLYTVGPGQIHMDGDLALW
                     YARSRSKSSDFDRGRRQQEVIRTIFQKALQTGTFTKIPQLYNDFSSTVVTDMSLGDML
                     SLAPYAVNFTNANIRSYYIRPPYVLPWTTPGGAAVLLPQDSLSSMLVEATTLSTYAAT
                     RETVTVEVQNGSYFDTMETLAASRLNYAGYETITSPADNRNYGSSVLVDYTVAQDPSQ
                     RQTIIDVLGTYSANVLSMPDPNSKVQYRVIIGADYEACFRPEDLAH"
     gene            3215..3288
                     /locus_tag="PLT08_04445"
     tRNA            3215..3288
                     /locus_tag="PLT08_04445"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3249..3251,aa:Val,seq:cac)
     gene            3406..4044
                     /locus_tag="PLT08_04450"
     CDS             3406..4044
                     /locus_tag="PLT08_04450"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HNE03743.1"
                     /translation="MSSRTNETWLSDLRSSGPVHEEALSDLRAIIQKGLPYALSRWLS
                     SDDPLFPPLVEEVTQETLLRVLDQLDTFEGRSLFTTWVHKIAIRIALTELRRKRWRDA
                     SLDELTENEDAPPPPGLLADPHASPETSAERKDMVSRVRRILEEELTPKQREALVLLG
                     LQDMPMEDAAKRMKTNRNALYKLLHDARLRLKKRLALEELTPQDVLLAFEQK"
     gene            4112..4408
                     /locus_tag="PLT08_04455"
     CDS             4112..4408
                     /locus_tag="PLT08_04455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE03744.1"
                     /translation="MNNEHLPTPQNKPSQPEITDEVALRFLKILEQVREEDLSCSDMY
                     ARLDEFVEAEIKDGKDAAILTPLIHEHLDMCSGCCDEYEALLNVVENTSNTQDE"
     gene            complement(4438..4944)
                     /locus_tag="PLT08_04460"
     CDS             complement(4438..4944)
                     /locus_tag="PLT08_04460"
                     /inference="COORDINATES: protein motif:HMM:NF019533.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1684 domain-containing protein"
                     /protein_id="HNE03745.1"
                     /translation="MSELELFRAEKNEFFGSHPQSPLTREQKKDFHGLNYFPENETLR
                     LEVKVEEFSVKDELVMQTTTGGVQHYERFGKFSFDVEGQTVELTVYRSEHGFFLPFVD
                     ALAGSETYPAGRYLEPDPLPGGRFFIDFNLAYNPYCAYNEMWSCPITPAENRLKVPIR
                     AGEKLFHE"
     gene            5074..>5383
                     /locus_tag="PLT08_04465"
     CDS             5074..>5383
                     /locus_tag="PLT08_04465"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNE03746.1"
                     /translation="MNSRQKTVMIIEDEADAAELFSEMMRINGFRVIKMFSSAPAIPI
                     ISQEKPDIILLDVMMPDISGLEVLRYIRREPELASIPVIILSAKSMPGDIKTGLEAGA
                     S"
BASE COUNT         1436 a         1522 c         1114 g         1311 t
ORIGIN      
        1 cagaaaaaga attaaaagca accttgcaag aaatggcaat tttacagaac cagttggcag
       61 atgctaatat caagctttta gaattggaaa aagggaaaat cgctaccaag agcaccgagc
      121 aagcagaagt gctcgcttcc atttctcagg aacttcgtca gccgctttct tctattgtcg
      181 ggtatacaga cctgttactg ggcgagtcag tcggtattct cggtgcgtta cagcgcaaat
      241 ttgtggaccg catcaaggtt tccacagaac gcatccgcag cctgacagac gatatgatcc
      301 aaatcacaac gctggctacc gacctgtatt cgctcaatcc tgaagctgta gatttgaact
      361 caatcatcga caatgccatg tcgtacacga gctctcagat tcgtgagaaa aatatttcca
      421 ttcatctgga agttccaaag gcagtcacat ccatccacgc ggacagagaa gccctacaac
      481 aaattgtcat tcacttgttg caaaacgccg gagcggcaac tcccttcgaa gggacgatcc
      541 gcctcaaagt ccagacccag aacgaagacg gcgctgagta cattttgatt caagtcgcag
      601 atcaaggcgg cgggattgcc tccgaagata ttccgcgcgt ttttacccgt ctctatcgag
      661 ctgataatgt tctcattcaa ggggttggtg ataccggcgt tggtctttct attgccaaaa
      721 ccctcaccga agcccagcat ggtcgtattt gggttgaaag caaagtggga gaaggttcca
      781 cgtttagtgt gcttttgcca atctctggcg acaaacttgc cgacaagcaa aaaaggaaat
      841 aaacaatgcg aggcgtgcgc gattttggaa acgcgcttgt tgcggcaatt ctctcaattg
      901 ggttaacact gggagcgttg tcgatttctc ttgttggctt tatccctgaa gaagtcgcca
      961 ctccatcccc aacactgatt gtttctccta ttccggtaac tgctacaaac accttgcctc
     1021 caacggctac tttgttagtc accgttaata ttcctacgtc ttcaccaacc aataccattg
     1081 ttcctccaac ctcctgtcaa ccacctgtgg gctgggtggg agttgttgtt caagtaggcg
     1141 acacgttaga tagcctcgca gcaaggtatg gcacgtataa agacattctc aaaaacaaaa
     1201 actgcctttt cacagacagt cttattgccg gcacgcttct atatgtgccg ggtggtattt
     1261 ccaccagtac agtagttgct tgtaaccaag gcgctgttgg ttggatcaaa aactatatgg
     1321 ttcgcccagg cgataccttc tataacatcg gcactcgcta tggcgtcaac gccaacttaa
     1381 tcaaacttgt caactgccga actagcgaca ttatttatgc gggtgaaatt ctttatgtac
     1441 caaacgtacc cacacgcaca ccaactttta cacctttgcc tggtattacc tacaccgcgg
     1501 caccattact caccgaacca ttaaccctaa cggttctgcc tttcacggca acatacccgc
     1561 caacccaaac atccactgcc accaccgcac ctacggcttc gcccattcca acccaaaccg
     1621 ccagcttaac cgctatcccc accccaacgc cctaagcagg cgcactttcc ataacacatc
     1681 gatgaaatca tccaaccttt ttgtatgggc gctttgcctt gccgttttta tttcagcttg
     1741 cggggcagca gctccgaccc aagcgcctca gccttttgtt ttaatcactg ccgctccaaa
     1801 cgcatcgcct acaccaacac cctttcaacc ttccttagcc agcccaactg caccttttaa
     1861 tacaagcgga tcaaaccccg ccccgcaacc gattcttgca actgaaacta cattcccaac
     1921 tgccacgcct caagcttcac cgacagcgac agcccccatt gagcagttat tccccaccca
     1981 agccgctcct cctcctgccg aagccttggt aaaccccaca gccttaccac ccctcaccac
     2041 cgaaaacgac accattaatt tcctgctagt cggctcagac cgtagaccaa ccggaacctc
     2101 tcaccgcaca gacacattac tcattgctgt ggtttggtac aaagaaggtc aagtctccct
     2161 catctccatt ccgcgcgata cttggctcta cattccaacc gtaggcatgc aacgcatcaa
     2221 caccgcttat caaagcggag aaggcgtgta ccccggcggc gggatgggac ttttgaaaga
     2281 caccattctg caaaacttcg gtatccgtat cgaccataca gccatggtgg agtttgatgg
     2341 ctttcgccgc attgtagata ccctcggtgg gatcgatgtc cccatatcct gcccctatac
     2401 cgattggcgt ctcatctccc ccggtctgga cgaaaatgac gaaaacaact ggtggctata
     2461 cacagtcgga ccaggtcaaa ttcatatgga tggcgatctc gccctttggt acgcccgctc
     2521 ccgttccaaa tccagcgact ttgaccgcgg aagacgtcaa caagaagtga tccgaaccat
     2581 cttccaaaaa gcccttcaaa ccggtacatt cacaaaaatt ccgcagctct acaacgattt
     2641 ctcaagcaca gtcgtcactg atatgagcct tggcgacatg ctcagcctcg caccctatgc
     2701 agtaaatttc accaatgcca atattcgcag ctactacatt cgcccgccct acgtcctgcc
     2761 gtggacaacg cccggcggag cagccgtcct tctgccacaa gactcattaa gctctatgct
     2821 ggtcgaagcg acaactcttt cgacttatgc cgcaactcgc gaaaccgtca cagtagaggt
     2881 gcaaaacggc tcatactttg acacgatgga gaccctcgcc gcctcacgct taaattatgc
     2941 tgggtatgaa accatcacca gcccagcaga caaccgaaac tatggcagct ccgttctggt
     3001 tgattacact gttgctcaag atcccagtca acgccaaacc atcatagacg tgttgggcac
     3061 ctattcagcc aatgttttgt ccatgccaga cccgaacagc aaggttcaat atcgcgtcat
     3121 cattggcgca gactatgaag cctgttttag acccgaagac ctggcacatt aggcttgcaa
     3181 aagatgagag gctcggctat aatctcgctc cgttgggtgc gtagctcagt tggttagagc
     3241 actactatca cacagtaggg gtccggggtt cgagtccctg cgcgcccaca gaaaaccgtc
     3301 tccttgaggc ggttttttct tttccgaaac ggatttataa aaatactatc agaatctctc
     3361 agggatgctg aaaaacccct tgcagtatct tataatgact acgttatgtc ttctcgcact
     3421 aatgaaactt ggttaagtga tttacgcagt tctggtcccg tccatgaaga ggcgttaagc
     3481 gacttgcgtg caatcatcca aaagggtttg ccgtacgcac tatcacgctg gctatcgtca
     3541 gacgatcctc tctttcctcc cctcgtcgaa gaagtaaccc aggaaaccct cctacgcgta
     3601 ttagaccaat tggatacctt tgaagggcgc agccttttca ccacctgggt acacaaaatt
     3661 gccatccgta tcgcattgac cgagcttcgc cgcaagcgct ggcgtgacgc gtctctcgat
     3721 gagctgacgg aaaatgaaga cgctcctcct cctcccggtc tgcttgcaga ccctcatgca
     3781 tcccctgaaa cctctgccga acgcaaagat atggtctctc gtgtacgccg catcttggaa
     3841 gaagagctga ccccaaaaca gcgagaggcg ctcgttttgc ttggcttgca agacatgccc
     3901 atggaagatg ccgcaaaacg catgaaaacc aaccgcaatg cattatacaa actgcttcac
     3961 gatgcccgcc tgcgcctcaa aaaacgccta gcgctggaag aactgacacc gcaagatgtt
     4021 ctactcgcct tcgagcagaa ataatttctt atcaattggg tataattgac aaagtaagat
     4081 taagttttta ttaatcgtct caccctttag aatgaataac gaacatctcc ctacccctca
     4141 aaacaagcct tctcaacctg agattacgga tgaagttgcc ttgcgattct tgaagatcct
     4201 tgagcaagtt cgtgaagagg atctctcttg cagtgacatg tatgcgcggc tggatgaatt
     4261 tgtggaagcc gagatcaaag acggtaaaga cgccgccatt ctcacgccac tcatccatga
     4321 gcatttagat atgtgttccg gctgttgtga cgaatatgaa gctttactga atgttgtaga
     4381 aaatacaagc aatactcaag atgaataaaa aaaatgacgg gctagcccgt cattttttta
     4441 ttcatggaat agtttctcac cagcccgtat gggcaccttc aagcggttct cagccggggt
     4501 gattgggcag gaccacatct cattgtaggc acagtatggg ttgtatgcca agttgaagtc
     4561 aataaagaag cgcccacctg gcaaagggtc tggctcaagg taacgcccgg cggggtacgt
     4621 ctcactacca gccaacgcat ccacgaacgg cagaaaaaat ccatgctcgc tgcgataaac
     4681 ggtcaactct acggtttgcc cctccacatc aaaagagaat tttccaaagc gttcatagtg
     4741 ctgaacacca cctgtggtgg tttgcatcac caactcatcc ttaactgaaa attcttccac
     4801 tttcacttcc agccgtaaag tttcattttc tggaaaataa ttcaacccat gaaagtcttt
     4861 tttctgctca cgggttagcg gactttgcgg atggctgcca aaaaactcat tcttttctgc
     4921 acgaaaaagc tccaattcac tcattgatac ctccaaatct tattttgcat agacatggaa
     4981 caaatccgaa ttcttacaag gacatcgcaa aaatcatgcg cccttgcaaa ttccatacct
     5041 ttatgctagt tttagcatag tagaggaaac aaaatgaatt cgagacaaaa aacggtcatg
     5101 atcatcgaag acgaagccga tgccgctgaa cttttttcag aaatgatgcg catcaacggg
     5161 ttccgtgtta tcaaaatgtt ctccagcgct cccgccattc ccatcatctc acaagaaaag
     5221 ccagacatta ttttgctcga tgtaatgatg cccgatattt caggtcttga agtgttacgc
     5281 tacatccgcc gcgaacctga actcgcctcc attcccgtca ttatcctttc agccaaaagc
     5341 atgcccggcg atatcaaaac cgggcttgaa gctggggctt caa
//
LOCUS       DAOQJE010000117         5376 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_8848_length_5376_cov_5.697049, whole genome shotgun sequence.
ACCESSION   DAOQJE010000117 DAOQJE010000000
VERSION     DAOQJE010000117.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 5376)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5376)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5376
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8848_length_5376_cov_5.697049"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..718)
                     /locus_tag="PLT08_04470"
     CDS             complement(<1..718)
                     /locus_tag="PLT08_04470"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNE03747.1"
                     /translation="MSLPVIYVENLTRRFGDFVAVDHINFEVNEGEVVGYLGPNGSGK
                     TTTIRMLLGLLAPSEGEATVLGYDVHRQSEEVRARVGYMSQKFAIYDDLTTLENLTFY
                     GGVYGITDKARIMETLELVGLSGHEHTLTRDLSTGWRQRLSLGIALVHKPKLLFLDEP
                     TSGVDPTARRTFWDLIYQLADNGVTILVTTHYMDEAEYCERVGIMRDGKLLAMDTPSN
                     LKRDIVPGDVWEVYAEPLEQG"
     gene            complement(719..1153)
                     /locus_tag="PLT08_04475"
     CDS             complement(719..1153)
                     /locus_tag="PLT08_04475"
                     /inference="COORDINATES: protein motif:HMM:NF022072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HNE03748.1"
                     /translation="MINIDLGVLIDRPVKDVFAFVSNPNNMTKWNSAVINLEPLTPGT
                     VGVGAKFKTTGEAMGRRIEGEMQITAYEPDTKCGFQVTAGPMQVNLTMTFKTVGTGTK
                     VSLNAQGNPGGLFKLAEGVMAGQVKSMMEGNLSRLKSALEGS"
     gene            complement(1169..2131)
                     /locus_tag="PLT08_04480"
     CDS             complement(1169..2131)
                     /locus_tag="PLT08_04480"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNE03749.1"
                     /translation="MDDYLVNATGLKKSYGANHAVNGVSLRIPPGEIYGLVGADGAGK
                     TTTMRLLVGALLPDAGEISVCGYDVLKQTEQARSTIGYLSQRFSMYEDLTVLENIRFF
                     AEIRGLSSTEWLPRSLEILDFVGLEKFKDRRAGQLSGGMKQKLGLASALVTRPRLLLL
                     DEPTTGVDPVTRQDFWQLVIKLVGSREGDGVSVIISTPYMDEASRCHRVGFMKNGRII
                     TEDTPSNLRSLLNGRILELRGSPLNTLRHVAHTDVDVEDVQAFGDRLHIRVRDDKANQ
                     VLRRLKSSIKAAGGHVVELRMVPPALEDVFIALSEDKAISAVSV"
     gene            complement(2198..3508)
                     /locus_tag="PLT08_04485"
     CDS             complement(2198..3508)
                     /locus_tag="PLT08_04485"
                     /inference="COORDINATES: protein motif:HMM:NF027890.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HNE03750.1"
                     /translation="MQNKLPPLPVRIVIAVIVLGTLAYFGFQSLKDDTNGSLTASGTI
                     EATIVNVAPETAGKVTDVRADEGESVTKGQSLLSLDASLLTAQQAVASAAVDSATAAL
                     AAAQTKYDQTLEAALAAQAAQRSKDWQASAPSEFDQPNWYIEQDQQVSSAQAELDAAK
                     AEIDAANENLDRVITDLKNSEFLKTEQELADARAAFFIAQQVKDQASSASDNSGLTDA
                     AQDYYDDAVTRLNDAEEAYNDLMNSDEADDVRYARGQVLVAQQRYDAAYARLLTLQTG
                     ENSPAVIAAGKELDKAKSALAQAEASLALVQAQIAKLEVHAPMDGVILTRNVEPGEFV
                     QPGAAALTIGDLSDLTITVYVPENRYGEISLGQTAEVTVDSFPGVTFTATVIQIANKA
                     EFTPRNVQTVEGRSATMFAIKLSVQDAEGRLKPGMPADVIFISK"
     gene            complement(3550..4680)
                     /locus_tag="PLT08_04490"
     CDS             complement(3550..4680)
                     /locus_tag="PLT08_04490"
                     /inference="COORDINATES: protein motif:HMM:NF024091.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNE03751.1"
                     /translation="MFNTRIVSIVRKEFVQIFRDPRSLALIIVMPIIQLFLLGYAATT
                     DVKNVPIAVLDQSQSAQSRELLDAFRAADYFRIDYVVGSTEEYQLLIESGKIRAALII
                     PPDYDNQLADGKAQVLFVLDGSDGSVGATALSTARLIGQSYSTKVQTSQFALRGSGSL
                     PTQPVEVRTQVWYNPDFQSAYFMIPGVIGMILSFITTILTATTIVRERERGTIEQLIV
                     TPIRSWELIVGKLLPYVILAFVETFEILIVGHLAFDVPVRGSLLLITLASGLFLMSSL
                     GIGLFASTIANTQQEAMLSVMMFNLPMIFLSGFFFPVQAMPQFLQFVSLAIPLRYYLV
                     VIRALMLKGVGFAAIQSEIISLTIFGIVIMGIAALRFRKRLD"
     gene            complement(4685..5293)
                     /locus_tag="PLT08_04495"
     CDS             complement(4685..5293)
                     /locus_tag="PLT08_04495"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HNE03752.1"
                     /translation="MAEETHTKGERTRIQIEDAAIELFIEQGYHATSMRQIADRADLA
                     LGGIYNHFKSKDEIFEAIIVDKHPYKRLLPLILEAEGHTMEDFLGHAARVVIKELTSQ
                     PYYIKLMLIEIAEFNGVHGAVLIKEIGPKLLPVFERIIKTRKNLRLTNPAMLMRTFIG
                     MVVSYMITEMLISNSMLNKMMPKNPIDAYVDIYLHGIVKSEA"
BASE COUNT         1399 a         1338 c         1341 g         1298 t
ORIGIN      
        1 gcccttgttc caagggttcg gcatacactt cccatacatc gccggggacg atgtctcttt
       61 tcaggttgga gggggtatcc attgccagca atttgccatc gcgcatgatg cccacgcgtt
      121 cgcaatattc ggcttcatcc atgtaatgcg tggtgacgag gatggtgacg ccgttatcag
      181 ccagttggta gatcaaatcc cagaaggtgc ggcgagcggt ggggtccacg ccagaggtgg
      241 gttcatctag gaagagcaat ttcggtttgt ggaccagtgc aattccaagt gagagcctct
      301 gccgccagcc ggtggagaga tccctcgtca gtgtatgctc atgcccagag agaccgacca
      361 gttccaacgt ctccatgatg cgggccttgt cggtaatccc atacacgcca ccgtaaaagg
      421 tcaaattctc caacgtggtt aggtcatcat aaatcgcaaa cttctgcgac atatacccaa
      481 cccgtgcgcg gacttcttcg ctttggcggt ggacatcgta ccccaatact gttgcttcgc
      541 cttcactggg ggcaagcaaa ccaagcagca tgcgaatggt agtggttttg cccgagccgt
      601 tgggtccaag ataaccaacc acttcacctt cgttcacttc aaagttgata tgatccaccg
      661 cgacaaaatc gccaaaacgc cgggtgagat tttcaacata aataacaggc agtgacattt
      721 acgatccttc caatgcagat ttgaggcgag aaagattgcc ttccatcata gatttgacct
      781 gccccgccat gacaccttca gccagtttga ataaaccgcc ggggtttcct tgtgcgttca
      841 agctaacttt tgtacctgta cctactgttt tgaaggtcat ggtcaggttc acttgcatgg
      901 gtccggcagt gacttgaaag ccgcacttgg tatcgggttc ataggctgta atttgcattt
      961 caccttcgat gcgccgcccc atggcttcgc cggtcgtttt gaacttcgca cctacaccaa
     1021 ctgtgcccgg cgtgagcggc tctaaattga tgacagctga attccatttt gtcatgttgt
     1081 tcgggttgga aacaaaagca aaaacatctt tgacagggcg gtcgatcaat acacctaaat
     1141 cgatgttaat catggcaggc tccttttatt aaaccgatac agcagagatg gctttatctt
     1201 cagataaagc gataaacaca tcttccaagg cggggggcac catacggagc tcaaccacat
     1261 gcccgcccgc agctttgata ctggacttca agcggcgcaa tacttgattc gccttatcat
     1321 cacgcacacg gatgtgaagc cggtcaccaa aggcttgcac atcttccaca tccacatcgg
     1381 tatgggcaac atgccgcaga gtattcaaag gtgagccgcg caattcaagg atacgtccgt
     1441 ttagaagtga acgcaaatta gacggggtat cttccgtaat aattctgcca tttttcataa
     1501 acccaacccg atggcaacga gaggcttcgt ccatgtatgg tgtggagatg atgaccgaaa
     1561 cgccatcacc ttctcgtgaa ccaaccagtt taatgaccag ttgccaaaaa tcctgacgag
     1621 tcacagggtc tacgccggtg gtgggttcgt cgagcaagag caaacgtgga cgagtgacca
     1681 atgccgaggc aagcccaagc ttttgcttca tgccgccaga aagttgcccg gcgcggcgat
     1741 ctttgaattt ttccaaaccg acaaagtcca gaatctccaa cgaacgcggc agccattcgg
     1801 tggaagaaag cccgcggatc tcagcaaaga aacggatatt ctccaacacg gtcaagtctt
     1861 catacatcga gaagcgctgc gaaagataac caatggttga gcgggcttgt tcggtttgct
     1921 tcaacacgtc gtatccgcaa accgaaattt ctccggcatc tggcagcaac gccccaacca
     1981 gtaagcgcat tgtggtcgtc ttacctgccc catctgcacc gacgagtcca taaatttcgc
     2041 cgggagggat acgcaaagaa acaccattca ctgcatgatt agcgccatat gattttttta
     2101 atccagttgc gttaactaga taatcatcca taatttctac cttcaactgg tggtttcgat
     2161 acgctccata aaaaacgcgg agcgactcaa ccaccgatta tttactaata aagataacat
     2221 ccgcaggcat accgggtttc aaacgtcctt cggcatcttg cacactgagt ttgatcgcga
     2281 acatggtggc actgcgtcct tccacagttt gcacattgcg cggagtgaac tcggctttgt
     2341 tcgcaatttg gatgacggtg gcagtgaaag tcacgccggg gaaagagtcc acagtcactt
     2401 cggcggtctg cccaagggag atctcgccgt agcgattctc tgggacgtac actgtgatgg
     2461 tcaggtctga gagatcgccg atggtaagag ctgccgcacc cggctggaca aattctcccg
     2521 gctcgacatt acgggtgaga atgactccgt ccattggggc gtggacttcg agtttagcaa
     2581 tctgtgcttg gaccaatgcc aggctggctt ctgcttgtgc cagtgcagac tttgccttat
     2641 ccaattcttt tccagccgca atcacggcag gagaattttc gccggtctgt aaggtgagca
     2701 agcgggcata ggcggcatca taacgctgtt gcgccacgag gacttgtccg cgggcatagc
     2761 gcacgtcatc ggcttcgtcg ctgttcatca agtcgttata ggcttcttca gcgtcattga
     2821 ggcgggtaac agcatcgtcg taataatctt gggcggcatc ggttaatccg ctattatctg
     2881 aagcgctgga cgcctgatct ttcacttgtt gtgcaatgaa aaatgcagca cgagcatcgg
     2941 caagttcttg ttctgttttt agaaactcag aattcttcag gtctgtaatg acacggtcca
     3001 ggttttcgtt ggctgcatca atttcggctt tggcggcatc aagttctgct tgagccgatg
     3061 aaacctgctg gtcttgttcg atgtaccaat tgggttggtc aaactcactt ggagcagaag
     3121 cctgccaatc tttggagcgt tgtgcagctt gggcggcaag agcggcttcg agcgtttgat
     3181 catatttggt ttgggcagcg gcaagggcgg ctgtggcaga atctactgcg gcagaggcaa
     3241 cggcttgttg ggcagttaac aggcttgcgt cgagggagag aagcgattgt cccttcgtca
     3301 ctgattcgcc ttcatcggcg cggacatccg tcactttgcc agcggtttca ggggcgacgt
     3361 tcacaatggt cgcttcgatg gtaccagaag cagtcagact gccattggta tcatctttga
     3421 gcgattgaaa gccgaagtat gccagcgtgc cgagcacaat cacagcgata acgatgcgga
     3481 cgggcagagg gggtagttta ttttgcatga gagactccag aattgaaagt taaaagtaca
     3541 agttgaaaat tagtcgaggc gttttctaaa gcgcaaggca gcaatgccca taataacgat
     3601 gccaaaaatg gtcagggaaa tgatctcgct ttgaatagca gcaaagccta cgcccttaag
     3661 catgagtgcg cggatcacca caaggtaata gcgcaatgga atcgccagag atacgaactg
     3721 caaaaactgc ggcatggctt gcactgggaa gaaaaagcca gagagaaaga tcatcggcag
     3781 gttgaacatc atgaccgaca gcatggcttc ttgctgggtg ttagcaatcg tagaagcgaa
     3841 caagccaatg cctaaactgg acatgagaaa caagcccgaa gccagcgtaa tgagcagtaa
     3901 gcttccgcgc acgggcacat caaacgcaag atgcccgacg ataagaattt caaaggtctc
     3961 cacaaaagct aaaatgacat agggcaacaa cttccccacg atcaactccc acgagcggat
     4021 tggcgtaaca atgagctgtt cgatcgtgcc gcgctcgcgt tcacggacga tggtagtagc
     4081 tgtgagaatg gtggtgatga aagaaaggat catcccgatc acaccaggga tcatgaagta
     4141 tgccgattga aaatcggggt tgtaccaaac ttgagtgcgt acttccacag gttgtgttgg
     4201 taatgaaccc gaaccacgca gtgcgaattg gctcgtttgc accttggttg agtacgactg
     4261 accaatcaac cgcgcagtgg aaagtgccgt tgcgcctaca ctgccatcgg aaccatctaa
     4321 cacaaaaaga acctgcgctt tgccatctgc cagttgatta tcataatcag gcgggatgat
     4381 gagcgcagca cggatcttgc ccgactcaat cagcaattga tactcttccg tagaacccac
     4441 cacataatca atgcggaagt aatcggcggc gcgaaaagca tctaacaatt cacgtgattg
     4501 agcgctttgg ctttggtcaa gcacagcgat aggcacgttc ttcacatccg tggtggcggc
     4561 ataccccagc aaaaacagct gaataatggg cataacgatg atcagcgcca gtgaacgcgg
     4621 gtcgcggaaa atctgcacaa actctttacg aacaatcgaa acgatacggg tattgaacat
     4681 ggaatcaagc ctcagattta acaatgccat gcaggtaaat atcaacatag gcatcgatgg
     4741 gatttttagg catcatttta tttagcatgg aattcgatat gagcatttct gtgatcatgt
     4801 acgaaaccac cataccgata aaagttcgca tcaacatggc tgggttagtt aggcgcagat
     4861 tcttgcgagt ctttattatt cgttcaaaga ccggcaacaa cttgggaccg atctccttga
     4921 tcaacacagc accatgcacg ccgttaaact cggcgatctc aatcagcatc aacttgatgt
     4981 aatacggctg agacgtcagt tccttgataa ccacccgcgc cgcatgaccg agaaaatcct
     5041 ccatcgtatg cccttctgct tccaaaataa gcggtaaaag ccttttatat ggatgtttat
     5101 caacaattat ggcttcgaat atctcgtctt tactcttgaa gtggttgtaa ataccgccca
     5161 atgccaaatc tgcccgatcg gcaatttgac gcatcgatgt agcatggtag ccctgctcaa
     5221 tgaacaactc aattgcagca tcttcaattt gaatacgagt gcgctcgcct ttggtatgtg
     5281 tttcttctgc catgacgtct ccagaaaata aatgaacgaa cgttcgtttt tattttagac
     5341 caagtaggtt tattttgtca aggaggaaaa ataagt
//
LOCUS       DAOQJE010000118         5372 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_8858_length_5372_cov_6.066391, whole genome shotgun sequence.
ACCESSION   DAOQJE010000118 DAOQJE010000000
VERSION     DAOQJE010000118.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 5372)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5372)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5372
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8858_length_5372_cov_6.066391"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..313
                     /locus_tag="PLT08_04500"
     CDS             <1..313
                     /locus_tag="PLT08_04500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE03753.1"
                     /translation="DNVVLITGTVLDINNNPINGIEISISRDEQSDRTLTSKEGTYYL
                     YLPTTLSGVWSLKQTAISCESNLMGSDCKCLNSSCGAFEPIEYRLQFPLAPGLYDFVV
                     K"
     gene            complement(366..611)
                     /locus_tag="PLT08_04505"
     CDS             complement(366..611)
                     /locus_tag="PLT08_04505"
                     /inference="COORDINATES: protein motif:HMM:NF013286.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NifU family protein"
                     /protein_id="HNE03754.1"
                     /translation="MSVSESAVRESQVRQLLENLSAYIEQFHGGTVEFISLAGNDLQI
                     RLGGACLNCPLLPATLHGWVEGTVHQFFPEIHVVASN"
     gene            complement(611..1069)
                     /locus_tag="PLT08_04510"
     CDS             complement(611..1069)
                     /locus_tag="PLT08_04510"
                     /inference="COORDINATES: protein motif:HMM:NF012257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HNE03755.1"
                     /translation="MLDNLPGIPLFENLNPEQRFTLMSVFEKFACSPGTVIFEQGDIA
                     KYLYLILRGKAVISYKPYDGPRIVLSRLKDGDVFGWSAVVGGKKYSSSVASETTLETI
                     RIRRELLQNLLTQTPETGKIIIDRLALNVSPRWKNAHEQIKPLIHEERKQ"
     gene            1223..1426
                     /locus_tag="PLT08_04515"
     CDS             1223..1426
                     /locus_tag="PLT08_04515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE03756.1"
                     /translation="MKKTKNLSSFEKLLLGLEKPEILEVDVPLRKGSACPNCNEGVLD
                     YNGMLQLECPSCGFVNGESGGCT"
     gene            1426..>1673
                     /locus_tag="PLT08_04520"
     CDS             1426..>1673
                     /locus_tag="PLT08_04520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE03757.1"
                     /translation="MTEINSREETNELDIMEQEIPANTIPLGHIMALVMIGFGFLALG
                     IAFLLLRNQDEVSTGSSIDFSTVPAKVDFPAPDLSFID"
     assembly_gap    1674..1773
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            1847..2317
                     /locus_tag="PLT08_04525"
     CDS             1847..2317
                     /locus_tag="PLT08_04525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HNE03758.1"
                     /translation="MDVTLNWQGKMAFEGAGPSGFVQRLDSDASAGGDNSGARPMEMI
                     AIGLAGCTAMDVISILSKKQQKVADFQIKLHAERASDHPKVFTDSVIEYHVYGMDIDE
                     KSLVRAIELSAEKYCPAQAMLSKAFPIRLIYKIFDAETKSLLKEGEYQPKQKVA"
     gene            complement(2331..3821)
                     /locus_tag="PLT08_04530"
     CDS             complement(2331..3821)
                     /locus_tag="PLT08_04530"
                     /inference="COORDINATES: protein motif:HMM:NF013630.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HNE03759.1"
                     /translation="MKKITKIKNSIQTVTLVAVLFLSSCTSLPNNQTVASTSLPNSAV
                     EQSAVATAAFPTRRPYAPGELVDYVAQTGDTLPTLALRFNTSVDEILEANSFIPRDAT
                     TMPQGMPMKIPIYYRALWGSAFQIIPDHAFINGPTNIGFNTAAFLSTQTGWLSTYRAY
                     VGGEWKTGAALVDYAAINYSISPKLLLAILEYQGGALTQPAPPVNKNLLGLKRPFWDS
                     PYLQLVLAANTLNNGYYGWRTGTLLEFEDDDAILIRPDPWQNPGSVALQYYYSRMFTG
                     NDYNRAIGPDGLYKTYATFFGNPWENQFDLIPGSLSQPELSLPFPGDQTWSFTGGPHT
                     GWGTGFPFAALDFAPPAEQSGCVPAKPENFATAVADGLVVRSAQDGLALDLDRDGDER
                     TGWVIFYLHLATDQRATLGADLKTGDMIGYPSCEGGRSTGTHVHIARKYNGEWIPADS
                     ALPFVISGWVPHNGIEAYQGTLTKGGLTVTACVCGDAYTAISANYP"
     gene            complement(3805..>5372)
                     /locus_tag="PLT08_04535"
     CDS             complement(3805..>5372)
                     /locus_tag="PLT08_04535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HNE03760.1"
                     /translation="WGVVPTPTPNASQTAPLTDPFAGQSDPIVFPTSTIPPFIETENA
                     QTPTPQVTEETVTTQVEPSITTAPLNDAAPFLYYAQSGDMLSAVAARFGVSESEIISD
                     ADLTKTTLIDPGTLLVIPNRINEPTTPNVQLLPDAEFVFSATSIGFDTEKFVKDQNGY
                     LSSFRDYLGSVGWVQGYDAIDRLSVENSVSPRLLLALLEYEARWVRGQPIDLLHTEFP
                     MGFNDYHYKGMSVQMTWAINNMSIAYYGWRAGTITHIEFPDGTRLRLDPRLNAGTVAI
                     QYLFSKLHSESQWSQIINPDSGFPALYNEMFGDPWARADLVGPIFPPGLIQPPLVLPF
                     EPGAKWSFTGGPHNGWGQISPSIYGQSHSIYSAIDFAPAAAKSGCVPNDAWVVAAAPG
                     LVIRSENGVVMVDLDGDGYEQTGWDLLYLHLATKDRVPAGTYLELNDRIGHASCEGGV
                     ATGTHLHFARKFNGEWVTADGPIPFVMSGWRVVAGSAPYKGQLVKGDQVVTADTLSQA
                     WSNIIREKDEEDN"
BASE COUNT         1406 a         1125 c         1335 g         1406 t
ORIGIN      
        1 agataatgtg gtattgatta ctggaactgt attggacata aacaataatc ccataaatgg
       61 gattgagatt agtatctcca gagatgagca aagtgaccgt acgctaactt caaaagaagg
      121 aacttattat ttgtatttac ctacaaccct aagcggggtt tggtctctaa aacagacagc
      181 aataagttgt gaaagtaatt tgatgggttc agattgcaaa tgcctgaatt caagctgtgg
      241 agcttttgag cctatcgagt atcggcttca gtttccgctt gcgcctgggt tgtatgattt
      301 tgtagtgaag taggtaggtg cccagatggg tcttatgtta attaaattta ttgcagggtt
      361 cttttttaat tacttgctac tacatggatt tctggaaaga actgatgaac tgttccttcc
      421 acccagccat gtaaggttgc tggaagcaat ggacagttca agcaggctcc tcctaaacgg
      481 atctggaggt cattccctgc aagcgatatg aattccacag tcccgccgtg gaattgctca
      541 atgtaggcgc tcaaattttc caacaactgg cgtacctgac tttcacgaac ggctgactca
      601 gaaacagaca ttattgcttc ctttcttcgt gaatgagcgg tttaatttgt tcgtgggcat
      661 ttttccaacg aggagagaca ttcaatgcca atcgatctat gatgatcttt cccgtttccg
      721 gcgtttgtgt gagcaggttt tgtagcaatt cgcgacggat acgaattgtt tctagagttg
      781 tttcgctggc tacactggaa gaatattttt tcccgccaac tacggcagac cacccaaata
      841 catccccatc tttaaggcgc gaaagcacaa tccgcggacc atcataaggc ttatagctaa
      901 tgactgcttt accccttaat atcaggtata aatatttggc tatatcgcct tgttcaaaga
      961 tgaccgtccc aggggaacac gcaaatttct caaacaccga cattaaagta aacctttgtt
     1021 cggggttgag attttcaaat aacgggattc cgggcaggtt atctagcatt tgaaatcatg
     1081 ttaaccattg tataaatacc ctaccagtat catccatcct aaaccggatg acattcatcc
     1141 tgttttctat ttgggtattt tcccttagtg tatactttgt gaaaacggta atttttactt
     1201 atgttagacc ttcagaatct tgatgaaaaa aacaaaaaac ctttcgtctt ttgaaaaact
     1261 attacttggg ttggaaaagc cagaaattct cgaagtggat gtgccgcttc gcaaaggctc
     1321 ggcttgcccg aattgtaatg aaggggtatt ggattacaac ggtatgcttc aattggaatg
     1381 cccctcctgt ggctttgtta acggggagag tggagggtgc acctgatgac cgaaataaac
     1441 tcccgtgagg agactaacga attggatatt atggaacaag aaatacctgc aaacacaatc
     1501 cctcttgggc atattatggc attggtgatg atcgggtttg gattcctcgc actaggaatc
     1561 gcttttttgc tgttgaggaa tcaggatgaa gtatccaccg gctcttccat cgacttttca
     1621 acggtgcctg caaaagtaga tttccctgcc ccagacctga gttttattga cttnnnnnnn
     1681 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1741 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnatgtgga ttgggggcat ttcaaaaaag
     1801 aatctagaaa aatatgttcc tgatgtgatt gaggagtaaa aataaaatgg atgtgacatt
     1861 aaattggcag ggcaaaatgg catttgaagg agcgggacct tctggctttg tacaacgctt
     1921 agattcagat gcttctgctg gcggcgataa tagtggcgca cgccccatgg aaatgattgc
     1981 tataggtctg gctgggtgta cagccatgga tgtgatctca attctttcta agaagcaaca
     2041 aaaggtggca gattttcaaa tcaaacttca tgcagagcga gccagtgacc atcctaaagt
     2101 ttttacagac tcggtgattg agtaccatgt ttatggtatg gatattgatg aaaagtccct
     2161 tgtccgtgct attgaacttt cggcagaaaa atattgccct gcacaagcca tgctttccaa
     2221 agcttttccg attcgattga tatataaaat atttgatgca gagacaaaat ctctgttgaa
     2281 ggaaggcgaa tatcagccaa aacaaaaggt cgcgtaagcg accttttgtt ttatggataa
     2341 tttgcactga tggcggtata agcatcaccg catacacagg cagtgacagt taatccgcct
     2401 ttggtgaggg tgccctgata ggcttctatg ccattgtgcg gcacccagcc actaattaca
     2461 aagggtaatg cgctatctgc aggaatccat tcgccgttgt attttctggc aatatgaacg
     2521 tgtgtcccag tagaacgccc gccttcacaa gacgggtaac caatcatgtc gcctgttttt
     2581 aagtctgcgc cgagcgtggc gcgttgatcg gtggcaagat gtaaataaaa aattacccaa
     2641 cctgtacgtt catcaccgtc acggtcgagg tcgagagcca agccatcttg agcagaccga
     2701 acgaccaacc cgtcggctac agcggtggca aagttttcag gttttgcagg tacacagccc
     2761 gattgttcgg caggaggcgc gaagtccaac gctgcgaatg gaaaacctgt gccccagcca
     2821 gtgtggggtc cacctgtgaa gctccaggtt tgatctcctg ggaatggcag agaaagttct
     2881 ggctgcgata aactgccggg aatcagatcg aattgatttt cccaagggtt gccaaagaaa
     2941 gtggcatatg ttttataaag cccatctgga ccaatcgctc gattgtaatc attgcctgtg
     3001 aacatacgtg agtaatagta ttgaagcgca accgaacctg ggttttgcca tgggtctggg
     3061 cggatgagaa tggcatcgtc atcttcaaat tcaagcagag tccctgtgcg ccagccataa
     3121 tatccgttgt tgagtgtgtt agcggcaagc acaagttgta aataaggtga atcccagaag
     3181 ggacgcttca gcccaagcag gtttttgtta acgggcgggg caggttgtgt aagcgcaccg
     3241 ccttgatatt caagtattgc caataataac ttggggctaa tgctatagtt aattgccgca
     3301 taatctacaa gagcggcgcc tgttttccat tctccaccta cataggcgcg gtaggtggaa
     3361 agccagccgg tttgagttga aagaaacgct gcggtgttga aaccaatatt ggttggtccg
     3421 ttgataaagg catggtctgg gatgatctga aatgcactac cccaaagcgc gcgatagtag
     3481 atagggatct tcattggcat gccttgtggc atggtggttg catcacgggg aataaatgag
     3541 ttcgcttcaa gaatttcatc tacgctggta ttgaagcgta aagcaagggt cggtagggta
     3601 tctccggtct gagccacata atctactaat tcacccgggg cgtatggacg acgagttggg
     3661 aaagcagcag ttgccacagc agattgctcc actgcgctat ttggcagtga agtggaagca
     3721 actgtttgat tgtttggcaa ggatgtacaa gaggaaagga acaagacagc tacaagagta
     3781 accgtctgaa tggagttttt tattttagtt atcttcttca tctttttcgc ggattatatt
     3841 tgaccaagcc tgactcaatg tatctgctgt gacgacttga tcgcctttga caagttgacc
     3901 tttataaggg gcgcttcctg caacgacccg ccaaccgctc attacaaatg gaataggacc
     3961 atccgctgtg acccattcgc cattaaattt acgggcaaag tgaaggtgtg ttccggtggc
     4021 aacaccgcct tcgcaagaag catgaccgat ccggtcgttg agttcaaggt aggtgcctgc
     4081 agggacgcgg tcttttgtag ccaagtggag atataaaagg tcccagccgg tttgttcata
     4141 gccgtcgcca tcaaggtcta ccatcactac tccattttct gagcgaatga ctaaccctgg
     4201 agccgctgcg acgacccagg catcatttgg gacacagcca gattttgccg cggcgggagc
     4261 aaagtcgatg gcggaataga tactatgcga ctgcccataa atacttgggc taatctgccc
     4321 ccagccgttg tgtggaccac ccgtgaagct ccatttagcg cccggctcaa acggcaggac
     4381 aagcggtggt tgaattaagc cgggagggaa aatgggaccg actaaatctg cgcgtgccca
     4441 agggtcaccg aacatttcgt tgtacaaggc tgggaagccg gagtccggat tgataatttg
     4501 tgaccactgg gattcactgt gaagctttga aaataaatat tgaatggcaa cggtgcctgc
     4561 attgagacga gggtcaaggc ggagtcttgt accgtcgggg aattcaatat gcgttatggt
     4621 gccagcgcgc caaccgtaat aggcaatcga catgttgttg atcgcccatg tcatctgtac
     4681 actcatgcct ttgtaatgat agtcgttaaa acccattggg aattcagtgt gtaacaagtc
     4741 tatgggctga ccgcgtaccc agcgcgcttc gtattcaagc aaagccagca acaagcgcgg
     4801 gctgacagag ttctcgacgg aaagacgatc aatggcgtcg taaccctgta cccaaccgac
     4861 agaccctaag taatcacgaa acgaactgag atacccgttt tggtctttaa caaatttttc
     4921 tgtatcaaaa cctatagacg tggcagagaa aacaaattca gcatctggca aaagctgaac
     4981 attgggcgtt gtgggttcgt tgatgcggtt tggaatgaca agcagggtgc cggggtcgat
     5041 tagggttgtt ttggtgagat ctgcatctga aataatctca gattcgctca caccaaagcg
     5101 agctgcaacg gcagagagca tatctccgct ttgggcatag tatagaaaag gtgcggcatc
     5161 attaagcgga gccgtggtaa tggatggttc cacttgggtt gtaacagttt cttcagtaac
     5221 ttgtggtgtt ggcgtttgtg cattctccgt ctcaatgaaa ggcggaatgg ttgaggtggg
     5281 gaaaacgatc gggtcactct gaccagcgaa cgggtccgta agaggcgctg tttgagaggc
     5341 gtttggggtt ggggtgggaa caactcccca aa
//
LOCUS       DAOQJE010000119         5355 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_8894_length_5355_cov_6.441132, whole genome shotgun sequence.
ACCESSION   DAOQJE010000119 DAOQJE010000000
VERSION     DAOQJE010000119.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 5355)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5355)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5355
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8894_length_5355_cov_6.441132"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..236
                     /locus_tag="PLT08_04540"
     CDS             <1..236
                     /locus_tag="PLT08_04540"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNE03761.1"
                     /translation="QIFISISCQGRQLWVSIADDGKGFKSTSIEKQKPEDGSGLGMKT
                     MKERAESLGGKLRVTSAPKQGTTIELTIPLKES"
     gene            239..880
                     /locus_tag="PLT08_04545"
     CDS             239..880
                     /locus_tag="PLT08_04545"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HNE03762.1"
                     /translation="MKAIRVLIVDDHEIVREGLQTLLSEEKEIEVVGTAGDSDEAVSM
                     VKRHKPDVVLMDVILPGVDGIETTRRVVATNPSTRVLMLTSYSDDQRVREAIQAGAIG
                     YLLKDVLKADLLNAIRSAAAGKPALHAEAQQFLMRQLTGSDSAPHTRLTAREFNILQL
                     IAAGKSNKEIALALHLTEGTVKGYVSTIFDKLEVADRTQAALYAVEHGLLKPK"
     gene            1042..3309
                     /locus_tag="PLT08_04550"
     CDS             1042..3309
                     /locus_tag="PLT08_04550"
                     /inference="COORDINATES: protein motif:HMM:NF021778.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YncE family protein"
                     /protein_id="HNE03763.1"
                     /translation="MPSKHSNFIFRVAIVLMLVFISSCGGAPSDSETAPESSSGVIAK
                     NSSYSSPIAISDDDSLIVVANTLNSTVSLINVAGDANTKLTEVQVGEEPRSVVITPNK
                     AFAYVTNQVSGTVSVIDLNSQTKVTDITVGAEPYGIALTPDGTRAYVANSASNTVSII
                     DTAINTVVGTINIPGVQPRGVAITNNNGGTGQQFVYVTQFLSQPTASGGPGLDQGSEG
                     KVFVISTTDDAQIQGVITLAAHDTGFTADRTAFGGTDKEPTFAYPNQMQSIILKNGRG
                     YLPNVAASPEGPVKFNVDTQAFLSVFDVAAKSELAGGTINLHAAVKAQTFTPKLFLAN
                     PWAIAFKHNANEGYVVSAASDVLVKITIDDNGVPTVVSVPADGDTTRVLTIDVHKNPR
                     GIVINNADTRAYVYNFISKDVSVVDLTASPEVELARVQSADLPAAGSDAAKFLAGNEL
                     FHSSRGEFDEGVAERMSNEGWQACASCHPDGLSDGVVWAFNSGPRKSVPLNGTFSHEN
                     PTGNQRVLNYSAVFDELEDFELNIRNVSGGQGLIIQSGTTDPDPNVKAFDPPNANREQ
                     LHVGGFPAWDAIVAWTQFRVPSPVSPYRGVDPSSELGQQIAQGRDLFTQANCQACHGG
                     PKWSTPQVDFARVSPFPETITAGKPPEAPIGQLSRFLQIVGTFDPANPLEKNNNNQNA
                     LGTLGYNPPSLLSINAFPPYLHNGSCLTLDCVLENQTHREAGGVTGVLDDPANREALV
                     TFLKSIDITTEPITP"
     gene            3370..4812
                     /locus_tag="PLT08_04555"
     CDS             3370..4812
                     /locus_tag="PLT08_04555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE03764.1"
                     /translation="MNTFLKSRIWTIFLLVSLLIVACGGSATEAPVATEAPATEAAPT
                     GPTYTIIGAVNRDATQAAQAPFVTVDAQKNLLVVWAENSSGGVRQIFASQLNGTAFQP
                     LGSSINIHTNVLADSPSITVAGENNAVPWVAWSEPSPGFDNVAQVFASRFSVETGLWQ
                     QAGTDRGGGEASLNISTERAGTHPFIFSGSTEIGNPPVPWVAWEEVSDVNPSSVQIFV
                     AKGVKDESAIGGFRWETVGQVKEDGEQTLNVNRFRISQHPTGVFAETSNSVPWVTWQE
                     QGRDEPSRIFTARGVADPNTPGGFKWINVPPCDPKDETKCALNINPLNDAKDASMASG
                     STVPGEGNVPWIAWPEIGQNGKWQIFVSRLDTSTRNSFLQVGGSLNIDPNHDAKLPTI
                     VFVGNVPYVAWLEDDGTGKFEVQMRHLASDPQTGTWALDTPAEGFNKDKTLSDFNLSV
                     VASTDTVFLAWGEGDPATSASQMVIGAFRP"
     gene            4834..5244
                     /locus_tag="PLT08_04560"
     CDS             4834..5244
                     /locus_tag="PLT08_04560"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HNE03765.1"
                     /translation="MPLRLLMHPAHPARDSYRILIVDDAPTVRESLKWLLEDESGLTV
                     IGDAGNGDDAIQMAVNLKPDLVLLDIELPDMDGFAVAKQIKAMLNPPRIILLSMHNDA
                     VSKKRGAEAGCDAFVGKEFGWGVLLPVLRTVLES"
BASE COUNT         1351 a         1330 c         1365 g         1309 t
ORIGIN      
        1 gtcagatttt tatatcaatt tcttgccaag gcaggcaatt gtgggtttcg attgcagatg
       61 atggaaaagg cttcaaaagc acgtccattg agaaacaaaa gcccgaggat ggcagcgggt
      121 tgggaatgaa gacaatgaaa gagcgggcag aatcactggg tggcaaactc cgtgtgacat
      181 ctgccccgaa acaaggtacg accattgaat tgaccatacc tcttaaggag agttagagat
      241 gaaagctatt cgtgtcttga ttgtggatga ccatgaaatt gtccgcgagg gattgcagac
      301 tttgctaagc gaagaaaaag aaatcgaagt ggtgggcact gcgggggata gtgatgaagc
      361 cgtctctatg gtgaagcggc ataaaccgga tgtggttctt atggacgtga tcttgccagg
      421 tgtggatggt attgagacaa cccggcgagt cgttgccacc aacccatcta ctcgggtgtt
      481 gatgctaaca tcgtattctg acgatcagcg agtgcgagaa gccattcaag caggcgcgat
      541 tggttattta ttgaaagatg tgctcaaggc agatttatta aatgcgattc gatctgccgc
      601 tgccggaaaa cctgccttac acgcagaagc gcagcaattt ctgatgcgcc aactcaccgg
      661 ttcagattct gcgccgcata caaggttgac cgccagagag ttcaatatct tgcaattgat
      721 cgccgccggg aaaagcaaca aagagatcgc gctcgctttg cacctcacag aagggactgt
      781 gaaaggctat gtcagtacga ttttcgataa attggaagta gcagaccgaa cacaagccgc
      841 gctttatgcg gtggaacatg ggctattgaa gccgaaataa atcgccttcc tccgtctgat
      901 tctgtaccta actttggtta gccgtaatgg ctaacctttt tcttttgatt caatggctct
      961 tgatggtttg ttttgaaagg gctttcttct atgattatga gtatcccgtt atcggtatcc
     1021 attatataga aggagagaag tatgccatcg aaacattcaa attttatttt tcgagtcgca
     1081 attgtgctta tgcttgtatt cataagttcg tgcggcgggg cgccgtctga ttctgagact
     1141 gcgccagagt caagcagtgg ggtgatcgca aagaattcga gctattccag cccgattgcc
     1201 attagtgatg atgactcact catcgtggtt gcgaatacgc ttaacagcac ggtgtcgctg
     1261 atcaatgtag caggcgatgc caacaccaag ttgaccgagg ttcaagtagg cgaagagccg
     1321 cgctctgtgg tgatcacgcc gaacaaagcc tttgcttatg taaccaatca agtcagcggt
     1381 acggtctcgg tcatcgactt gaattcacag accaaggtca ctgatatcac cgttggagct
     1441 gagccgtacg ggatcgcgct cacccccgat ggaacccgtg cctatgttgc aaattccgcc
     1501 tcgaatacgg tttcgatcat tgatacagca atcaatacag ttgtcggtac gatcaatatt
     1561 cccggcgtgc aaccacgtgg ggtagcgatc accaataaca acggaggaac cgggcagcaa
     1621 ttcgtttatg taacccagtt cctctcccaa cccaccgctt caggtggacc aggtttagat
     1681 caaggcagtg aaggcaaagt ctttgttatt tccacaacag acgatgcaca aattcaaggc
     1741 gtgatcacgc tcgcagcgca tgatacaggc ttcaccgcag atcgaaccgc atttggcggc
     1801 acagacaaag aaccaacctt tgcgtatccg aatcagatgc aatccatcat cttgaaaaat
     1861 ggacgcggct atttacccaa cgtagcggct tctcctgaag gacctgtgaa gttcaatgta
     1921 gatacacaag ccttcctttc ggtattcgat gtagctgcaa aatctgaatt ggcaggcggc
     1981 acgatcaatt tgcatgcggc tgttaaagca caaaccttta cgcccaagtt attccttgcc
     2041 aacccatggg caattgcctt caagcacaat gccaatgaag gctatgtcgt ttcagcagcg
     2101 agtgatgtat tagtcaagat cactatcgac gataacggtg tgcctacggt agtttctgtg
     2161 ccggcggatg gagatacaac gcgcgtcttg acgattgatg tccacaaaaa tccgcgcgga
     2221 atcgtaatca acaatgcgga tactcgcgca tacgtttaca acttcatttc caaagatgtc
     2281 tctgtagtgg acctcaccgc ctcacccgaa gttgagctgg ctcgtgtcca gtcggcagat
     2341 ttgcccgctg caggttcaga cgccgcaaaa ttcctggctg ggaatgagtt attccattct
     2401 tcacgcggtg agtttgacga aggcgtggcg gaaagaatgt ccaacgaagg ttggcaagct
     2461 tgtgcaagtt gtcaccccga tggcttgagc gatggtgtag tgtgggcgtt caattccggt
     2521 ccgcgcaaga gcgtgccctt gaatggaacc tttagccatg agaaccccac tggcaatcag
     2581 cgtgtgttga attactccgc agtgttcgat gagttggaag actttgaatt gaacattcgt
     2641 aacgtctctg gcgggcaagg cttgatcatt caaagcggta ccaccgaccc agacccgaat
     2701 gtaaaagcct ttgacccacc caatgccaac cgtgaacaac tgcatgttgg cggcttcccc
     2761 gcttgggatg cgatcgttgc gtggacacaa ttccgtgtac cttcccccgt ttccccttat
     2821 cgtggcgtag atccaagcag tgagcttggt caacagatcg cacagggacg tgacctcttc
     2881 acgcaagcga actgtcaggc ttgccatggt ggacccaaat ggtccacgcc tcaagtggat
     2941 tttgctcgcg tttcgccctt cccagaaacc atcacagccg gaaaaccacc cgaggctcca
     3001 attggtcaac tctcacgctt cttgcagatc gtcggcacct ttgaccctgc caacccgctt
     3061 gagaagaaca acaacaatca gaatgcgctt ggaacattag gttacaaccc gccttctttg
     3121 ttgagcatca atgccttccc accttatctc cataacggct cctgcctgac cttggattgt
     3181 gtcttggaaa atcaaaccca tcgcgaagcg ggcggcgtga ccggcgtatt ggatgaccca
     3241 gcaaaccgtg aagcattggt caccttcctc aaatccatcg atatcaccac agagccaatt
     3301 accccgtaaa tttgttcgtc acatttcagc gagagggaga caactctctc tcgctgaagg
     3361 agaaccctta tgaatacatt cttgaaatca agaatatgga cgatcttctt actcgtaagt
     3421 ttattaatcg tagcctgcgg cgggtctgca acagaagcgc ctgttgccac cgaagcccct
     3481 gctacagaag ctgcccccac cggacccacc tacaccatta ttggtgctgt taaccgggat
     3541 gcaacccaag cggctcaagc tccattcgta acagtggatg cgcaaaagaa tctactggtt
     3601 gtttgggctg agaattcctc tggcggagta cgccaaatct ttgccagcca attgaatgga
     3661 actgcctttc aacctttggg ctcctccatc aatatccaca ccaatgtgct tgcagattcg
     3721 cccagcatca ctgttgcagg tgagaacaat gctgtgcctt gggtggcgtg gtctgagccc
     3781 agccccggtt tcgacaatgt ggcgcaggtg tttgcgagtc gcttcagtgt agagacaggt
     3841 ttatggcagc aagcaggcac agacagaggg ggtggcgagg cgagtctcaa catctcgaca
     3901 gagcgcgcag gcacgcatcc atttattttc tcagggtcca cagagatcgg gaatccgccc
     3961 gttccgtggg tggcatggga agaagtctcg gatgtgaacc catcttctgt gcaaattttt
     4021 gtagccaaag gcgtaaagga tgaaagtgcg attggcggct tccgatggga gaccgttggg
     4081 caagtgaaag aagatggcga acagactttg aatgtcaatc gcttccgcat ctcacagcac
     4141 cctacgggcg tgtttgctga aacgtcgaac tcggtaccgt gggtaacctg gcaagaacag
     4201 ggacgtgacg aacccagccg catctttaca gcacgtggtg tggcagaccc caacacgccc
     4261 ggtggtttca agtggatcaa tgttccgcct tgtgacccga aagatgagac caaatgcgcg
     4321 ctcaacatca atccgttgaa tgacgcgaaa gatgcttcca tggcttctgg cagcaccgtt
     4381 cccggtgaag gcaatgtgcc atggatcgca tggcccgaaa ttggtcagaa tggcaaatgg
     4441 cagatctttg tctcgcgttt ggataccagc acgcgtaata gttttcttca ggttggcggc
     4501 agtttaaaca ttgacccgaa tcatgatgcc aagttaccga ccattgtgtt tgttggaaat
     4561 gttccatatg tggcttggtt agaagatgac ggcacaggca aatttgaagt gcagatgaga
     4621 cacctcgcct cagacccaca aaccggaact tgggcgctcg ataccccagc cgaaggcttc
     4681 aataaagata aaaccctttc agatttcaat ctctctgtgg ttgcttctac agatacggtc
     4741 ttccttgctt ggggcgaagg tgaccctgca acctctgcct cgcaaatggt tatcggcgca
     4801 ttccgcccat aacgagctga tatgcagaac tccatgcccc tgaggcttct catgcaccct
     4861 gcccaccctg caagagatag ttaccgaatt ctgatcgtag atgatgcgcc taccgtacgt
     4921 gaaagtttga agtggttgtt ggaggatgaa tcggggttga ccgttattgg cgatgccggg
     4981 aatggcgacg atgccattca aatggcggtc aaccttaagc ctgatctggt tctgctcgat
     5041 atcgaattac cagatatgga cggctttgcc gttgcgaaac agatcaaagc catgctcaac
     5101 ccgccgcgga tcattctgct ttccatgcat aacgatgcgg tctctaagaa acgaggtgca
     5161 gaggcaggct gtgatgcttt tgtaggaaaa gaattcggtt ggggtgtgtt gttacctgta
     5221 ttaaggacag ttttggaaag ttgatacaaa aaaggaggtc acgttttgaa cgtgacctcc
     5281 tttttatttc gtagaaattt ctacttcagc gctcattccc cacatcaagc catcgggttg
     5341 ttcatccaat tggat
//
LOCUS       DAOQJE010000120         5351 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_8904_length_5351_cov_7.637840, whole genome shotgun sequence.
ACCESSION   DAOQJE010000120 DAOQJE010000000
VERSION     DAOQJE010000120.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 5351)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5351)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5351
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8904_length_5351_cov_7.637840"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..956)
                     /locus_tag="PLT08_04565"
     CDS             complement(<1..956)
                     /locus_tag="PLT08_04565"
                     /inference="COORDINATES: protein motif:HMM:NF013871.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE03766.1"
                     /translation="MEEQNNTNHSPYAGRWVALVRGRVVAQGGTPEQALRASQSSRYK
                     ERPEIRFMSVPFNLPPLLEKIVNVLPANTEIYLVGGAVRDLLTNRRSPDFDFAVPSNG
                     ISLARTVANALKADFMVLDGERDTGRVIIIEDGTYTYLDFAAYRGATLEEDLRDRDFT
                     INAIALNLRDITVIDPLNGATDIRAKVIRACSPDSLSNDPVRILRAVRQAAAFGFSID
                     KSTREWMKQAADKINNVSIERVRDEVFKMLGGPKASASIRALEMLGVLQHLMPELLKM
                     KGVTQSNPHIHEVWVHTLAVVDQLEDLLTSSLRLDFDPEKTS"
     gene            complement(978..1535)
                     /gene="hpt"
                     /locus_tag="PLT08_04570"
     CDS             complement(978..1535)
                     /gene="hpt"
                     /locus_tag="PLT08_04570"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006166 - purine ribonucleoside salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HNE03767.1"
                     /translation="MQNYQDFLAEVLIDTASLQKRVAELGKQISADYNGQDLLLICIL
                     RGAVPFMVDLSRHITVPHMMDFMAVSSYGIGRRESSGSARVTLDLQMDIRDHNVLLVE
                     DIVDSGHTIAAVLQMLGTRQPKSLKVCALLDKPERREAHVPIDYLGFSIPNKFVFGYG
                     LDLDEYHRNLNFVGVVDLDRYKPVQ"
     gene            complement(1605..2174)
                     /locus_tag="PLT08_04575"
     CDS             complement(1605..2174)
                     /locus_tag="PLT08_04575"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004797 - thymidine kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HNE03768.1"
                     /translation="MRHTHGLIEVVCGSMFSGKTDELIRRLVRATIAKQKVQVFKPAI
                     DIRYAVEKVASHTGSTFDAIPVEKASEILSKVAADTTVVGLDEAQFFDAEIVDVAQTL
                     SDRGIRVIVAGLDMDFRGEPFGSMPVFMAKAESVLKLHAICMVCGDEASRTQRLVNGK
                     PARYDEPVVIVGASELYEARCRLHHEVPR"
     gene            complement(2276..2572)
                     /locus_tag="PLT08_04580"
     CDS             complement(2276..2572)
                     /locus_tag="PLT08_04580"
                     /inference="COORDINATES: protein motif:HMM:NF015102.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha
                     C-terminal domain-containing protein"
                     /protein_id="HNE03769.1"
                     /translation="MEEQKTREESTNVNNRSVDDLGLTPRATEGLKRAGILNIGQLVE
                     KFAEGDAALLAINGFGQSALTAAKKKLRAMDITLPEPPKVEKAEKAEKPAEAAE"
     gene            complement(2582..2785)
                     /gene="rpmE"
                     /locus_tag="PLT08_04585"
                     /pseudo
     CDS             complement(2582..2785)
                     /gene="rpmE"
                     /locus_tag="PLT08_04585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003151132.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L31"
     gene            complement(2785..3045)
                     /gene="rpmA"
                     /locus_tag="PLT08_04590"
     CDS             complement(2785..3045)
                     /gene="rpmA"
                     /locus_tag="PLT08_04590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002513859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HNE03770.1"
                     /translation="MAHKVGGGSSRNGRDSNSQRLGVKRYAGQFVLSGNVLVRQRGTR
                     IKPGLNVMVGKDDTLFATADGVVMFDVYRGKKRVNIVPAEVE"
     gene            complement(3066..3374)
                     /gene="rplU"
                     /locus_tag="PLT08_04595"
     CDS             complement(3066..3374)
                     /gene="rplU"
                     /locus_tag="PLT08_04595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HNE03771.1"
                     /translation="MKFAIVESGGKQYRAVEGTTIDVDRLALELGTKFDFERVLLVAD
                     GDNVSVGTPTLSVKVSATVVDHVKGPKTLAFKYRPKKRIRVRGGNRAHYTRLKIESIG
                     "
     gene            complement(3624..4100)
                     /locus_tag="PLT08_04600"
     CDS             complement(3624..4100)
                     /locus_tag="PLT08_04600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE03772.1"
                     /translation="MPTPIKPAVKPGSAIAKPGTVSYVKFSDKLTASVTDIAKIIEQH
                     KEMIDSIQEVAIELTSSIGSLHALTVKYARTANQILDVLLPILKNLPIIPKNVMQLLV
                     NLESLTQKIIDNEKVTAKTITDVNSGLKTGDANKLKAHAGQLKEVTKTITAIIPKA"
     gene            4249..5094
                     /locus_tag="PLT08_04605"
     CDS             4249..5094
                     /locus_tag="PLT08_04605"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF013506.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S49 family peptidase"
                     /protein_id="HNE03773.1"
                     /translation="MSDADVRWERIRNILLLAVLVIIGLLIGSAIFPQPVIGVIRLED
                     AIYTYSAQNTIKQIQYAIDHSEVKAVVFVIDSPGGTVVDTESIYMELTRLREKKPVVT
                     VVNGMSASGGYYLSANTDYIFAKPTSLVGNIGVIGYLPPAPFVIEDVITTGPYKLWGA
                     PRDSDMRQVEMIKQGFFDAVRFGRGDKLTAEENVTFSGQIWAGIDALKLGIIDEFGTE
                     NDGSKKAAELAKVANYEIVDLAAPAGIDMSGGFFFMASADGVTLPYPKEAGMYLLYIP
                     PLPVK"
     gene            5107..>5351
                     /locus_tag="PLT08_04610"
     CDS             5107..>5351
                     /locus_tag="PLT08_04610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE03774.1"
                     /translation="MKRQTIYIALAALILPILLRGLWFYRGLPAQRPEIATPDYASFE
                     RPAVSVSTPDLENVKQLGGSVLIDAAHTNQFTMTEIDS"
BASE COUNT         1339 a         1272 c         1374 g         1366 t
ORIGIN      
        1 tcgctggttt tttcagggtc aaagtctaat cgcagagacg aggtgagcaa atcttcaagc
       61 tggtctacca cagcgagagt gtgtacccac acttcgtgga tgtgcggatt ggattgggta
      121 acgcccttca ttttcaacaa ttcaggcata aggtgttgta aaacgcccaa catctctaac
      181 gcccgaatgg acgcgctggc tttgggtccg ccaagcattt taaagacttc atctcgtaca
      241 cgctcaatag aaacgttgtt gatcttgtct gccgcttgtt tcatccactc gcgagtggat
      301 ttgtcgatac tgaaaccaaa tgctgccgcc tgacgcaccg cacgcagaat ccgcaccggg
      361 tcattggaca aggaatcggg tgagcaggca cgaatcactt tggcgcggat atctgtcgcg
      421 ccgttcaatg ggtctatgac agtaatgtca cgtagattaa gcgcaatcgc attgatggtg
      481 aagtcacggt cgcgaaggtc ttcttcgagt gtcgctccac gataggcagc gaaatcgaga
      541 taggtatatg taccgtcttc gataataatg acacgtcctg tatcacgttc gccatcgaga
      601 accatgaagt ccgctttaag ggcgttggca acggttcgag cgagagaaat gccattcgaa
      661 ggcacggcaa aatcaaagtc gggagagcgg cggttggtca atagatcccg cactgcccca
      721 ccgacaagat agatttccgt gtttgcagga aggacattaa ctattttttc caataaaggg
      781 ggaaggttga agggaacaga cataaaacga atctctggtc tttctttata gcggctggat
      841 tgcgaagcac gcaaggcttg ttctggtgta ccgccttgag caacgacacg cccacgaacg
      901 agggcaaccc aacgccctgc atatggtgaa tgattggtgt tgttttgttc ttccaaaata
      961 ccccctgcac cgggcgctta ttgaacaggt ttgtaacgat cgagatctac gacgccaaca
     1021 aaattcaaat tgcgatgata ttcatcaagg tcgagcccat aaccgaagac gaacttgttg
     1081 ggaatactaa agccaaggta atcaataggc acatgcgctt cgcggcgttc gggcttatcg
     1141 agcaaggcgc aaacctttag cgatttgggc tgacgtgttc caagcatttg caaaacagca
     1201 gcaatggtat gaccactatc cacgatatct tcaacaagca aaacattgtg gtcacgaata
     1261 tccatttgaa ggtctaaagt aacacgagca gaaccgctgg attcacgcct tccgattcca
     1321 tatgaagaga cagccatgaa gtccatcata tgtggcacgg tgatgtggcg gctcaagtcc
     1381 accataaaag gcaccgcgcc gcgcagaatg cagatgagca acaaatcttg tccgttatag
     1441 tcggcactga tctgcttgcc cagttcggca acgcgtttct gtaaagaggc tgtatcaata
     1501 agaacttcgg cgaggaaatc ctggtagttt tgcatcaaag tattccttgg gtatttgagt
     1561 ttaattagtt tatcactact aaagcccggt gtggggcttc tccactaacg cgggacttcg
     1621 tgatgcaaac gacagcgggc ttcatacaac tcagaagcac caacgatgac caccggctca
     1681 tcataacggg caggcttgcc attcaccaga cgctgagtcc tagacgcttc atctccacaa
     1741 accatacaaa tggcatgcag cttcaacaca gattctgcct ttgccataaa caccggcatg
     1801 gagccgaatg gttcgccacg aaaatccata tccaaaccag caacgatgac acgaatgcca
     1861 cggtctgaaa gggtctgcgc cacgtccacg atctcggcat caaagaactg cgcctcatcc
     1921 aaacccacaa cggttgtgtc tgctgcaact tttgagagaa tttcactggc tttttccacg
     1981 ggaatggcat cgaaggttga gccggtatgt gaagcgactt tttccaccgc ataacgaatg
     2041 tcgatggcgg gtttaaagac ctgcaccttt tgcttggcaa tggtggcgcg tactagtcgt
     2101 cgaatcaatt catccgtctt gccgctaaac attgaaccgc aaacaacttc gatcaagccg
     2161 tgggtatgcc tcattgaaat tctcctgccc cgttcgggga aaatgttttt ttcataaaaa
     2221 caggcagacg cttttgcatc tgcctgttta gtttacctga taagaagcgg actgactact
     2281 cagcagcttc ggcgggcttt tcggcttttt cagccttctc taccttggga ggttcaggga
     2341 gagtaatgtc cattgcgcgg agctttttct tggcagccgt caaagcagac tgaccaaaac
     2401 cgttgatcgc gagaagagca gcatcgcctt cagcgaattt ttcaacaagc tgaccaatgt
     2461 tcaaaatgcc agcgcgcttg agaccttcgg tagcacgcgg ggtgagaccg agatcatcga
     2521 cagagcggtt gttgacattg gtggattctt cgcgggtctt ctgttcttcc acataggact
     2581 ggcgagcaga aagcttctta tagaagcggt ctacctgacc ttcgacatcg aggatcttag
     2641 cagattcacc ggtgtagaaa ggatggcagt tggagcacac gtccacgcgt aattctttct
     2701 tggtggaagt agtaatccaa gtgttgccgc aggaagcaca agtgaccttg gcatcgtaca
     2761 ctgtaggatg aatatccgct ctcattattc cacctcagcc ggaacgatgt tcacgcgctt
     2821 cttgccgcga tagacatcga acatcaccac accgtcggca gtggcgaaca aagtatcatc
     2881 tttaccgacc atcacgttca aacccggctt gatgcgggtt ccgcgctggc gcaccaacac
     2941 gttgccggaa agcacaaact gaccggcgta gcgcttaaca cccaagcgct gggaattgct
     3001 atctctaccg ttgcggcttg aaccaccgcc taccttatga gccattgtct acctaccttt
     3061 atttcttaac cgatggattc gatcttcaaa cgggtgtaat gggcgcgatt gccgccacgc
     3121 acacggatgc gtttcttcgg gcgatatttg aatgcgaggg tcttgggtcc cttaacatgg
     3181 tccaccacag tagcagaaac tttcacactg agggttggtg tacccacgga gacgttgtct
     3241 ccatcagcca cgagcaaaac acgctcgaag tcaaactttg tgcccaattc aagggcgagg
     3301 cgatccacat cgattgtggt gccttcgacg gcacgatatt gcttgccgcc gctttcaacg
     3361 attgcaaatt tcattttcta ttttctccag tcattcactt cgccgagaaa gttcgcgggg
     3421 atcttgcacc cttcgcctac ttctggggaa gctggggttg taaacaaaca actaccccgg
     3481 aatctgacct ccggggtgga agcggacggt attatacatg cagagtctgg gacttgtcaa
     3541 cgagatttgg gcgagggaga actgccaagg cggggtctcc tgcctggttg ggcgccgcag
     3601 gtttaggtcc agaaagaggc gggctaggct ttggggataa tagccgtgat ggtcttggtc
     3661 acttccttca attgacccgc atgcgccttc aatttattgg catcgcctgt tttcaagcca
     3721 gaattgacat cggtaatggt cttggcggtg accttttcat tgtcgatgat cttttgggtc
     3781 aggctttcaa ggttgaccaa caactgcatc acattcttgg ggatgatggg caggtttttg
     3841 agaataggga gcaacacatc gagaatctga ttcgcggtgc gggcatattt gacggtgaga
     3901 gcatgcaggg agccgatgga agaggtcaat tcgatggcaa cttcttgaat cgagtcgatc
     3961 atttctttgt gttgttcaat gatctttgca atgtctgtga ccgaggcggt taatttatct
     4021 gaaaacttga cgtaggatac ggtgcccggt tttgcaatgg cgcttccagg ttttactgca
     4081 ggtttgattg gggttggcat gagaaaatac ctcctgaaaa aatttgtgct gcacatgcag
     4141 caagatatgc caaatatact gtatatcgtt tcgcaaaaca accttgttga caaagcatgt
     4201 tttctcgtat atcctgaaag cgaattccaa cttatagagg agaaacccat gagcgatgcg
     4261 gatgtacgtt gggaacgaat tcggaacata ctgctattgg cagtccttgt aatcataggg
     4321 ttgctaatag gctcggcaat cttccctcaa ccagtcattg gggtgatacg gctggaagat
     4381 gcgatttata cctattcagc acaaaacact atcaaacaga tccaatacgc gattgaccat
     4441 tcagaagtaa aggcggttgt atttgtgatc gatagccccg gtgggacggt ggtggataca
     4501 gagtccattt atatggagct gacccgtttg cgcgaaaaga agccggtggt gacagtggtc
     4561 aatgggatgt ctgccagcgg tgggtattat ctttctgcta atacagacta tatcttcgcc
     4621 aagcctacgt cattggttgg gaatattggt gtgatcggct acttgccccc tgccccattt
     4681 gtgattgaag atgtcattac gacgggaccg tataagttat ggggtgcgcc gcgtgattcg
     4741 gatatgcgtc aggtggagat gattaagcaa ggcttctttg atgcggtccg ctttgggcgt
     4801 ggggataagc tgacagcgga agaaaatgtc acgtttagcg gacagatctg ggcggggatt
     4861 gatgcgctca agttgggcat cattgacgaa tttgggacgg aaaatgatgg ctccaagaaa
     4921 gccgcagaat tggctaaagt cgccaactat gaaattgtag atttggctgc tccggctggc
     4981 attgatatgt ctggcggctt tttcttcatg gcaagtgccg acggcgtgac attgccgtat
     5041 ccaaaggaag cgggcatgta tcttctttat attccgcctc ttccggttaa ataataggag
     5101 gatgaaatga aacgccaaac tatttatatt gcgctcgctg ctttaatcct ccccattctc
     5161 ttgcgtggac tatggtttta ccgtgggctt cctgcacagc gacctgaaat tgctacgcca
     5221 gattatgcat cttttgaaag accggctgtc tctgtgagca cgcctgactt ggaaaatgtg
     5281 aagcaacttg gtggcagtgt tttgatcgat gctgcgcata cgaatcaatt tacgatgacc
     5341 gaaattgact c
//
LOCUS       DAOQJE010000121         5275 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_9089_length_5275_cov_5.098276, whole genome shotgun sequence.
ACCESSION   DAOQJE010000121 DAOQJE010000000
VERSION     DAOQJE010000121.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 5275)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5275)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5275
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9089_length_5275_cov_5.098276"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            932..1132
                     /locus_tag="PLT08_04615"
     CDS             932..1132
                     /locus_tag="PLT08_04615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE03775.1"
                     /translation="MANSTSSPSVNFRLNAEDKQLLRELAKELRTSQVMAVRLVLREV
                     VPALKASREKRAAKQARSRLQS"
     gene            1192..2169
                     /locus_tag="PLT08_04620"
     CDS             1192..2169
                     /locus_tag="PLT08_04620"
                     /inference="COORDINATES: protein motif:HMM:NF012798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HNE03776.1"
                     /translation="MHSDFTPSLTHTSSNAQPAQGQFNMPSPFVLELGLFALLEMFIA
                     DRKAQGLAQKSIDFYEHNLNIFIDWCAAQGATNADQLNPDVIRSFFNHWKETGHSNGG
                     IHGVYRSVRALLRWFEQEYEPSGWRDPLRNIKPPRVDIEPLDPVSIEHVRAMVDTCNG
                     GKFTDYRDKAVMLFMLDTGVRAGELLALNIQDIDREGGVLVRKSKNRRPRTVFLGKEA
                     RRALRAYLKQRDDVWPALFVADDNERLKESGLRQILVRRAKRAQVPTPSCHSFRRAFA
                     LAMRRGGTQVLEIQKLLGHTDPKQIERYLKLDAEDLRDVHNRASPADAV"
     gene            2251..2577
                     /locus_tag="PLT08_04625"
     CDS             2251..2577
                     /locus_tag="PLT08_04625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE03777.1"
                     /translation="MQTHFTRSSTRDKSIYVKGRWIGKVIADTFKKTISSSLILHSPY
                     TAIANSVSVLNDAEALGAKYCEVFVSDTKQTYRATIKDIRAKGETCGSRDDPQIKLAL
                     SEWRRV"
     gene            2722..4020
                     /locus_tag="PLT08_04630"
     CDS             2722..4020
                     /locus_tag="PLT08_04630"
                     /inference="COORDINATES: protein motif:HMM:NF024872.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNE03778.1"
                     /translation="MMKTGAPTVDKFFVSRVEKPSKILTRRMMIDLIGRRVRGEVIDI
                     DPNSFSEPYKQCWLALDNAAPGKEFDTLKMIADESLLKQLAQQKAPTPYYPPMSEIQE
                     QIKDVTWYWDNRIPNGKLTVLGGESGVSKSMLAQRLCYLQLHEKQFPDGTPIHKPGEP
                     VLYVDCEGFASGIKKRAQAWQMDLSKFFLWSVDMENDGFINFSNPAFRDELIERVNSA
                     NPALVVIDSFGNASAGGQDKLEDVRDLLNFLNKVAYEFDLAILLIAHTRKPPAVFSGK
                     GEINQDDIRGSGHVVAMARSVIGVWLVQTGPEADPNGPRMMAVLKSNYARKPKPIGYD
                     VLNDANNNPMIFFGDAPKPYREPSKAQMCADWILETLTDEGAPMQPKELEVLAQKAGF
                     KRDTFYSAHRQLERGGLVKDTHGNKNPENAWALVDDREDG"
     gene            4200..5267
                     /locus_tag="PLT08_04635"
     CDS             4200..5267
                     /locus_tag="PLT08_04635"
                     /inference="COORDINATES: protein motif:HMM:NF013930.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="primase-helicase zinc-binding domain-containing
                     protein"
                     /protein_id="HNE03779.1"
                     /translation="MNANQINATFDLVAIVPDLRKAGRYHVGPCPFCGGNDRFTIKQS
                     EKGSIWHCRNCGDGKYHTPIDFIMRREDCDFKTALKIMGGEVVTRDTYTQQRMPTVQA
                     APPSWSRPDAEWRSHTWQDAAWKTIDSCSERLLNNHEGELGREYLASRGLHVGTWGQW
                     FLGFEYHYDPREKRKRPAIVIPWIDFERIDNMVVMALKYRFIDQLAKQDKSKRFTQRA
                     GSVSVLYGLGYVMPAHHTLLLVEGEFNCISVNQCVPAGVTCLSFGSEGGGDMDVMQAI
                     ATRHSRVFVWADDLWDNPRQVKRAGELQVLSRTGGRLRSAVEGADKFDANELLKRGAL
                     PGFLSQILGVQCLGHLGAVLG"
BASE COUNT         1446 a         1192 c         1380 g         1257 t
ORIGIN      
        1 agctggtgcc gacttgataa ccattcagaa gctattgggg catgcagatc tatctcagct
       61 catccgatat cttgcccaga cgccggatga tttgagagaa gctcatcagc gaggaagccc
      121 agccgataat ctgatttagc ctggaaggac gactcttgta ggctttgcaa tattgcagca
      181 tcacagaatg gatctaatgt ttgaggctgt gcctggtgtg ttatgacagg cgtgttgttt
      241 ctgtggaaac ggtcgccgga acagatagca aatttgttct attttcaagc cgagaatttt
      301 tttcagactt tgaaataccc tgccaccgcc caggaacgaa aagtcggcga gagtcggggt
      361 catcgattgt tttgaaaaat ggatctatag catgtgagag tctgcattgc atccaaggac
      421 tcccgcgtgc ctggcaattc aaaacgctga catcatcctg agtagggtca ttgaaagtgg
      481 agaccggtcc agagtcgttc tttgctttat agactctcac actcttggca attcagatgc
      541 cgacggcagc caacccaatg acttttgaaa tggcggtcaa ttaggagtcg tgctctatta
      601 tgcgacacct aaattcaatg gaccgtttct acagaaacga cagaaacgga acaccctaat
      661 tgctgattgt ttaggattga catcattgga ccgtcgcggt agtatagtga tacacacagg
      721 ggcatgaagt gtctataaag tggtcgctcg tgttgcgacc gatgtcggag ttacgcccga
      781 cgagtccaac gatttcgtgg caccactttc tataaatggc agttctggta tatccggaac
      841 gcaaaaagaa ataccggagt gatgccgccc gacgaaatcc aagtggattc gtcgggcgtt
      901 ttttgttaac caatttcgaa aggatatctg tatggcaaat tcaacttcat ccccctctgt
      961 gaacttccgt ttgaacgcgg aagataagca actcctgcgg gagcttgcta aagaattacg
     1021 gacatctcaa gtgatggctg ttcgcctggt gctgcgtgaa gttgtgccag ccctgaaagc
     1081 ctcaagggaa aagcgagcgg caaagcaagc aaggagccga ctgcagagct aacccattaa
     1141 acagcaaatc gcctgctagt aacaggcgac ttgccaaaag atgaatttcg gatgcattca
     1201 gattttactc cctccctgac tcacacgtca agcaatgctc agcctgcgca aggtcaattc
     1261 aacatgccat ctccattcgt cttggagctt ggcttgtttg cgctcctgga gatgttcatt
     1321 gccgaccgca aagctcaggg acttgctcaa aagtccatag atttttacga acacaattta
     1381 aatatcttta tagactggtg tgctgcgcag ggagcgacaa atgcagatca actaaatccg
     1441 gatgtcatcc gttccttctt caatcactgg aaggaaactg gtcatagcaa tggcggcata
     1501 catggtgtgt accgaagcgt gcgtgcgttg cttcgatggt tcgaacagga atatgaaccg
     1561 tctgggtggc gtgacccgct tcgtaacata aaacctccac gtgtggatat tgagcctcta
     1621 gaccctgtgt ccattgagca cgtgcgcgcg atggtggaca catgcaacgg cggcaaattc
     1681 accgattata gagacaaggc ggtaatgctc ttcatgctcg atacgggcgt gagagctggt
     1741 gaattgctcg ctctcaacat tcaagatata gacagagaag gaggtgtatt ggtccgaaaa
     1801 agcaagaaca gaagaccacg aactgtattc cttggtaaag aggcgcggcg tgcgctgcgt
     1861 gcttacctaa agcagcgtga tgatgtctgg cccgcgttat ttgtggcaga cgataacgaa
     1921 cgattgaagg agagcggact tcgtcaaatc ctggtgcggc gagcaaagcg tgcacaggtg
     1981 ccgacgccat cctgtcattc tttccgtcgt gcatttgccc tggcaatgag gagaggagga
     2041 acacaagtac ttgaaattca aaaactatta gggcacacag acccaaaaca aattgagcgt
     2101 tatttgaagt tagatgctga agacttgcgg gatgtacata accgcgcgag tccagcggat
     2161 gccgtctgaa aaccaaccca ttagaccgtt taaatgcgaa ctcgtctgct ggaagggcag
     2221 acgagcgcac ggctgaaaag gaaaatcaaa atgcaaactc attttaccag aagctcaaca
     2281 agagacaagt ccatttatgt aaaaggcaga tggatcggaa aggttatagc agataccttc
     2341 aagaaaacca tctcttcaag cctcatcctt cactcaccat atacagcgat tgcaaacagt
     2401 gtttcggtgc tgaacgatgc cgaagcctta ggcgcaaaat attgtgaagt atttgtcagt
     2461 gataccaagc agacctatcg cgcaacgata aaggatatca gggcaaaggg cgaaacctgc
     2521 gggagtcgcg atgatccaca aatcaaacta gccctttcag aatggagaag agtctaaggc
     2581 tgatcaattt aacgcgtctc gcctgctcat gcttgagcag gcgagcgcaa tttggagagt
     2641 tgcgaatgag acgatgacgg gtcaccggca ctctttccaa ttctacccaa aaatcttaaa
     2701 taagacactg gagaattaaa gatgatgaaa acaggtgcgc caacggttga taaattcttt
     2761 gtatcacgag tggaaaaacc atcaaaaata ttgactcggc ggatgatgat agaccttatc
     2821 gggcgtcgag ttcgcggaga ggtaatcgac attgatccaa attctttctc tgaaccttat
     2881 aagcaatgct ggcttgcact tgacaacgcg gcacccggga aagagtttga tactttgaag
     2941 atgattgctg acgagagtct cttgaagcag cttgctcagc aaaaggcgcc gacgccatac
     3001 tacccaccga tgtctgaaat ccaggaacaa attaaggatg tgacatggta ttgggataac
     3061 cgaattccga atggaaaatt gacagtcctc ggcggtgaga gcggtgtcag taaatccatg
     3121 cttgctcaac ggctctgtta ccttcaactt catgagaaac aatttccaga tggcactcca
     3181 atacacaagc caggggagcc ggtgctatat gttgactgcg aagggtttgc ttcaggcatt
     3241 aagaagcgtg ctcaagcctg gcaaatggat ctctcaaagt tttttctgtg gtcggttgat
     3301 atggagaatg acggttttat caacttctct aaccctgcct tccgggatga attaattgag
     3361 agagtcaact ctgcaaatcc tgccctggtt gtgattgact catttggaaa tgcgagcgca
     3421 ggaggacagg ataagttaga ggatgtcaga gacctcctga actttctcaa taaagttgcc
     3481 tatgagtttg atcttgctat tctgctcatt gcccacacac gtaaaccccc cgcagtattt
     3541 tcaggtaaag gcgagataaa ccaggacgac atacgaggca gtggtcatgt tgtggcaatg
     3601 gcgcgctctg tcatcggtgt ttggttggtt caaacaggtc cggaggcaga tccgaatggt
     3661 cctcgcatga tggcagttct caaatccaac tatgcgagaa agcccaagcc cattggctat
     3721 gatgttttga acgatgcgaa caacaatccc atgatcttct ttggagatgc tccaaagcca
     3781 tatcgggaac cgtcaaaggc acaaatgtgt gccgactgga tccttgagac attgaccgat
     3841 gaaggtgcac ctatgcagcc aaaggaattg gaagtcttag ctcaaaaggc tggcttcaaa
     3901 cgagatacct tttattcggc tcacaggcag cttgaaagag gcggattggt caaagatact
     3961 cacggcaata agaacccgga gaacgcttgg gcgcttgtgg atgaccgtga agacgggtag
     4021 ggaaatcgcg caagagtctg actgtatgac tgttttaaga ctttgcaata ggggggatgt
     4081 gaaaaagcaa tttcttgtta aaactgtcat acagtcatac agttccctcc tgttacggga
     4141 agtcacactc attttttgag actatgtatc gtaacaacgt aacgaattgg agtagacaaa
     4201 tgaacgctaa ccaaataaac gcaacctttg acctggtagc catcgtgccg gatctgcgca
     4261 aggctggtag gtatcatgtt ggaccctgtc cgttctgtgg cggtaatgac cggttcacca
     4321 tcaaacaaag cgagaagggg tctatctggc actgtagaaa ctgcggtgac gggaaatatc
     4381 acacgccgat tgacttcatc atgcggcgag aggattgtga tttcaaaacg gcattgaaga
     4441 taatgggtgg tgaggtggtg accagagaca catacactca acagcgcatg ccgacggtgc
     4501 aagcagctcc tcccagctgg agccgaccag atgcagaatg gcgctctcac acctggcagg
     4561 atgcagcatg gaagaccata gactcctgca gtgaaaggct gctcaacaat catgagggcg
     4621 aattgggaag agagtatctt gcctctcgtg gtcttcatgt tggaacatgg ggacaatggt
     4681 tccttggctt tgaatatcat tatgacccac gcgagaaacg aaagagacca gcgattgtca
     4741 tcccgtggat tgattttgag cgcatagaca acatggtagt gatggctttg aaataccgtt
     4801 tcatagacca actggcaaag caggacaaat caaagcgctt tacccaaaga gccggcagcg
     4861 tgtcagtgtt gtatggatta ggctatgtca tgccagcaca tcacacctta cttctcgtcg
     4921 agggagaatt caattgcatc agcgtaaacc agtgcgtacc cgcaggcgtg acctgtctct
     4981 cgtttggaag tgaggggggc ggtgacatgg atgtgatgca ggcaattgca accaggcatt
     5041 cacgtgtctt tgtgtgggct gatgatctat gggacaaccc acggcaagtg aagcgggcag
     5101 gggagctgca ggtgctatca aggaccggcg gacgcctgcg cagcgctgtt gagggtgctg
     5161 acaagttcga tgcaaatgag ctgctgaagc gtggcgcctt gcctgggttc ctctcgcaga
     5221 tccttggggt gcaatgcctt ggacatctgg gcgcggtcct tggttgaagt tcgcc
//
LOCUS       DAOQJE010000122         5251 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_9153_length_5251_cov_6.677829, whole genome shotgun sequence.
ACCESSION   DAOQJE010000122 DAOQJE010000000
VERSION     DAOQJE010000122.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 5251)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5251)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5251
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9153_length_5251_cov_6.677829"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            53..358
                     /locus_tag="PLT08_04640"
     CDS             53..358
                     /locus_tag="PLT08_04640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE03780.1"
                     /translation="MSQAPMMEVSSDDKLWAALAYAFSPLIPIILMFMEDKKNRPFIK
                     AHNMQALILGIITVVTSGFCIGILVWIYALYCAYQAYQGLEVKIPVLSDFVKNQGWA"
     gene            470..910
                     /locus_tag="PLT08_04645"
     CDS             470..910
                     /locus_tag="PLT08_04645"
                     /inference="COORDINATES: protein motif:HMM:NF021221.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4870 domain-containing protein"
                     /protein_id="HNE03781.1"
                     /translation="MMNEQIPQQPLAPLSPAEERQWAMFAHLGVLTNLFSGFLGPIVP
                     LAIYMIYKDRSRYVAYQSLQALIFQLIWWVGGGLLTGIVWAITGTLSAFIIGLICIPF
                     ACIISAMPLVALGYGTYAGIQCNQGQDFKYWLIGDWLRTTLTDN"
     gene            complement(947..2425)
                     /locus_tag="PLT08_04650"
     CDS             complement(947..2425)
                     /locus_tag="PLT08_04650"
                     /inference="COORDINATES: protein motif:HMM:TIGR00350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0070726 - cell wall assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HNE03782.1"
                     /translation="MDRFEFLKNIKRPTVSQIIFWVVTIALSITAFIVANNFTQCWTF
                     TKLPGIPPAKCGVTTDSTGFNPETGATQEAVLPDAPAEVSAPDVALPSWDGASRINIL
                     FIGLDERDLAANEGPPRTDSMIVFTIDPVSKTAGMLSIPRDMWVNIPGFGYSRINTAY
                     PSGEGAQLPGGGPELAKKTVSQFLGVPIHYYAQVDFNVFTKLVDELIAIGGCIYVEPT
                     EKMRLDPIGSGLDKIIITPGGARGLCEGWKVLAYARDRHTSGGDADRARRQQEVVLAL
                     KEIIFTPENFPKFFSAAPRMYSELSYGIKTDMAFEDALKLAVLGKDISIESIKTGVID
                     PQQGMAIFDNTTLNGEAASILKPVMDKIRVLRDEIFTSTGPTSPLAVPNGYTDPNLFA
                     QAMQQEEARVRVMDGTFTPGLDQRAGAVFQSYGMNVTEVAPSPEAYGQTIVIVYGPKL
                     YTIKWLQATFGISDNQIRFSPDPNQTVDIEIRIGSDIAGTIP"
     gene            complement(2438..3001)
                     /locus_tag="PLT08_04655"
     CDS             complement(2438..3001)
                     /locus_tag="PLT08_04655"
                     /inference="COORDINATES: protein motif:HMM:NF025030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HNE03783.1"
                     /translation="MKDLFAELTSGDDERAEQAIPAIVDAGAAAIPALLELTYSENTD
                     TRWWAVRALAASPHTQTSDLIPTLSDSSPEVRAAAALALSQHPGEEAVSALAQNLRDS
                     DPLAAGLAANALVKIGKAAVPSLIEAAKDGEQGIRILALRALSELRDHRAIPVMMQCI
                     QEDSAVLGYWAQMGLERLGLDMVYIKP"
     gene            complement(3008..3226)
                     /locus_tag="PLT08_04660"
     CDS             complement(3008..3226)
                     /locus_tag="PLT08_04660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE03784.1"
                     /translation="MVSTELLEILRCPSCVREKDGLLKLHKDAWLICQDCGRKYPIVD
                     DIPVMLIEEGDKWVNTAQDALPVPPPAK"
     gene            complement(3281..5155)
                     /locus_tag="PLT08_04665"
     CDS             complement(3281..5155)
                     /locus_tag="PLT08_04665"
                     /inference="COORDINATES: protein motif:HMM:NF016202.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanW family protein"
                     /protein_id="HNE03785.1"
                     /translation="MNAFIPQRDLPKQLLAVLSGGFVLFIGIVLVWTVGYQLAYAGRI
                     FPGVTVAGVDLSGLSPSEAAVKLSQTLSYPNTGKVLFRDGERVWVASPAELGMVFDPT
                     ASATAAYEHGRRGGLFASLSGQISARGLGMDIPPVVLFDQRVAYAYLQNIASQIDQPV
                     AEAILRVDGTNVIAEPGRVGRALNLDATLIYLGSQLQSFRDGEVSLVIQETSPLLLDV
                     TPQADVARAMLSQSFTMTLPNVGSGDPGPWTFDVPVLANMLVFNKVDAGGVPQLQVGL
                     DTNALHKSLSELKVVVDREPQDARFIFNESKGQLEAISASTIGRAVDVEASILAINGA
                     LQRGEHSVNLVVTEKQPAVTDQATGADLGIVQLVATQTTYFYGSSEARIQNIVTAAAQ
                     YDGLLVAPGEVFSMGSVLGDVSLENGYAEALIIYGGRTIKGVGGGVCQVSTTLFRTVF
                     FAGYPVVERYSHAYRVPYYEMDQSGSVDGNLVGMDATVYFPLVDFKFKNDTPYWMLME
                     TNVNVAARTITWKIYSTSDGRSVTWETTGAQNTVPAPETVFEENVDLKTNELKQVDYA
                     AEGADVTVTRTVWRNGAVYFTDQIQTHYEPWAAVCQYGPGTEDPRRQARKSEVCGFSS
                     "
BASE COUNT         1385 a         1311 c         1183 g         1372 t
ORIGIN      
        1 gattggttgt aaaatactta aaattcaaac aacttattcg gaggaataaa ccatgtctca
       61 agcacctatg atggaagtat cgagtgatga taagctgtgg gcagctcttg cttacgcctt
      121 ctcccccctt atcccgatca ttttaatgtt catggaagat aagaagaatc gtccattcat
      181 caaagctcac aatatgcagg ctttgatcct tggcattatc accgttgtca cttccggttt
      241 ctgcattggt attttggtct ggatctacgc tctgtattgc gcgtaccagg cgtaccaagg
      301 cttggaagtt aagatccctg ttcttagcga tttcgtcaaa aatcagggct gggcataatc
      361 agtctcgctc tacaaaagac ccgcgtgtaa cacgcgggtc ttttattttg ctactttgcc
      421 tctcctatac acgtattaat tcaaagagtt tgtatactat taggagaata tgatgaacga
      481 acaaatacct caacaaccac ttgccccgct cagccctgca gaagagcgcc aatgggcgat
      541 gttcgcccac ttaggagtgt tgacaaatct attttcaggt tttttaggtc ctattgtgcc
      601 attagcaatt tatatgatct ataaagatcg ttcccgctat gttgcatatc aatcgcttca
      661 agcgttgatt ttccaactca tttggtgggt cggtggcggt ttattgacag gtattgtgtg
      721 ggcaataacc ggcactttga gcgcctttat tatcgggcta atctgtattc catttgcttg
      781 tatcattagc gctatgccac tggtcgccct cgggtatgga acctacgcag ggattcaatg
      841 caatcagggt caagatttca agtattggct tatcggagat tggctccgca ccacattaac
      901 ggataactaa taaaaaatcc ccttcaaaag aaggggattt tttatattac gggattgtcc
      961 ctgcaatatc agaacctatt cggatctcaa tatctacagt ctggttcggg tctgggctga
     1021 agcgaatctg attatctgaa attccaaagg tcgcttgcaa ccatttgatc gtatatagct
     1081 ttggtccata cacaatcact atcgtttgtc catacgcttc aggggatgga gccacttcgg
     1141 tgacgttcat gccataggat tggaataccg ctccggcacg ttggtcaagc ccgggtgtaa
     1201 aggtgccatc cataacacgg acgcgggctt cttcttgctg catagcttga gcaaaaagat
     1261 tcgggtctgt atatccattc ggaaccgcca atggactggt aggacctgta gaagtaaaaa
     1321 tctcatcacg caaaacacga atcttatcca tcacaggttt gagaatgctg gcagcttccc
     1381 cattgagggt tgtgttatca aagatcgcca taccttgttg agggtcaata acacctgttt
     1441 ttatgctttc aatagaaata tctttgccaa gcacagcaag cttcaaagca tcttcaaatg
     1501 ccatatctgt cttgataccg tatgaaagtt cactatacat gcgcggagcg gcgctgaaaa
     1561 acttagggaa gttttcagga gtaaagataa tttccttaag cgctaagacc acttcctgct
     1621 gacggcgtgc acggtcagca tcaccaccgg aggtgtgacg gtcacgcgca tatgcaagca
     1681 ccttccagcc ttcgcacaac ccacgtgcac cgcctggtgt aattatgatc ttatccaatc
     1741 cagagccaat cgggtcaagc ctcatttttt cagtaggttc aacataaata cagccaccaa
     1801 tagcaatcaa ttcatctacc agttttgtaa acacattgaa atcaacttgg gcatagtaat
     1861 ggatcggcac gcccaagaat tgagaaactg ttttcttagc aagttctggt ccgccgcccg
     1921 gtaattgagc gccttccccg ctaggatagg ctgtattgat acggctatat ccaaaacccg
     1981 gaatgttcac ccacatatca cgcggaatag ataacatccc tgcggtcttg ctcacagggt
     2041 cgatggtaaa tacgatcatc gagtcagtac gtggcggacc ttcatttgct gcaagatcgc
     2101 gctcatccaa accgatgaat aagatattga tgcgactggc gccatcccat gagggtaatg
     2161 caacatcagg ggcagaaacc tcagccggag catcaggaag aacagcttct tgagtagcgc
     2221 ccgtttcagg gttgaaaccg gtgctatccg tggtcacacc acattttgca ggtggaatgc
     2281 ccggcaactt cgtaaacgtc caacactggg tgaagttatt tgccacaata aacgcggtga
     2341 tcgaaagagc aatagtcacc acccagaaaa taatctggga aaccgttggg cgttttatat
     2401 tttttagaaa ttcaaatcta tccattcact cattacctca aggcttaata tataccatat
     2461 ccaagcctag tcgttctaaa cccatctgtg cccaataccc caacacagct gagtcttctt
     2521 gaatacattg catcatgaca gggatagcac gatgatcgcg cagttctgag agtgctcgca
     2581 atgccaaaat acggatgccc tgctccccat cttttgccgc ctcaataaga cttggtacag
     2641 cagcttttcc gatcttgact aaagcattcg ccgcaagccc cgctgcaagt gggtcactat
     2701 ctctcaggtt ttgagccaac gctgaaacag cttcttcgcc tgggtgctga gacaatgcca
     2761 acgcggcagc ggcacgcacc tcagggctgg aatcacttag ggtgggaatc aggtctgagg
     2821 tctgggtgtg aggcgaagcg gcgagggcac gcactgccca ccaacgtgtg tctgtgtttt
     2881 cagagtaggt caattccaat aaggctggaa ttgccgccgc tcctgcatct acaatagcgg
     2941 gaatggcttg ttctgcgcgt tcatcgtctc cgcttgtaag ctcagcgaac aggtctttca
     3001 tttttattta ttttgcaggc ggcgggacgg gcaacgcatc ttgcgcggtg ttcacccatt
     3061 tatcgccttc ttcaatcaac atcacgggga tgtcatccac aatgggatat tttcgtccgc
     3121 aatcctggca gatgagccag gcatctttgt ggagtttcag caaaccgtct ttttcgcgca
     3181 cgcagctggg gcagcgcaag atttcgagta attcagtact gaccattgtt tctccttgtt
     3241 gggaaaagat tgtaccatgt aggttttgac aagattttat ttatgaacta aagccacaaa
     3301 cttcgctctt ccttgcttgt cttcgcggat cttctgtgcc gggaccatat tgacaaaccg
     3361 cagcccacgg ttcatagtga gtttgaattt ggtcggtgaa atatactgcc ccattacgcc
     3421 ataccgtgcg tgtgacagtt acatcagcac cttcagcggc gtaatctact tgtttaagtt
     3481 cgtttgtctt taaatctaca ttttcttcaa agaccgtttc tggggcaggg accgtatttt
     3541 gtgctcccgt tgtttcccaa gtgaccgaac gtccatctga tgtggaatag attttccacg
     3601 taatggtgcg cgcagctaca ttaacattgg tctccatcaa catccagtag ggcgtatcgt
     3661 ttttgaactt aaagtcgacc aaggggaaat aaaccgtggc gtccatgcca accaagtttc
     3721 cgtccactga gccgctttga tccatttcat aataaggcac acgataggcg tgagaataac
     3781 gttccaccac tgggtaccct gcaaagaaca ccgtgcggaa caaggttgtg ctcacctgac
     3841 acacaccgcc tcccacacct ttgatggtac gaccgccata aataatgagt gcttcggcat
     3901 aaccattttc caagctgaca tcgccaagca cactgcccat ggaaaacact tcgccaggag
     3961 caacgagcag accatcatac tgcgcagctg cggtgacaat attttgaatg cgcgcttcgc
     4021 tggacccgta aaaataagtg gtttgggtgg caacgagctg aacaatgcca aggtctgcgc
     4081 cagtggcttg atctgtcact gcaggctgct tttctgtaac aaccaagtta acgctgtgtt
     4141 ctccgcgttg taacgcaccg ttaattgcca gaatactagc ctccacgtcc acagcacgtc
     4201 caatcgtgga agcagagatc gcttcaagtt gtccctttga ctcgttgaag ataaagcgtg
     4261 catcttgagg ctcacggtct acaacgacct tcagttcact caacgatttg tgtaaagcgt
     4321 tcgtatccaa accaacttgt aattgcggca caccgcctgc atccactttg ttgaagacca
     4381 acatatttgc caaaacaggc acatcaaaag tccatggacc agggtcacca gaaccgacat
     4441 ttggtagtgt catcgtaaat gactggctaa gcatggcacg cgccacatct gcttgaggcg
     4501 taacatccaa caaaagtggg gatgtttctt gaatcacgag agaaacttct ccatctcgaa
     4561 aactttgcaa ctgcgaacca agatagatca aggttgcatc gaggttgagt gcgcgtccca
     4621 cacgccccgg ctctgcgatc acattagttc catctactcg cagaatagct tcagctacag
     4681 gctggtcgat ttgagaggcg atgttttgca aatatgcata tgcaacccgt tggtcaaaga
     4741 gcaccaccgg tggaatatcc atgccaagtc cgcgcgcgct gatttgtcct gatagtgaag
     4801 caaacaaccc accccgacgt ccatgttcat atgcagcagt ggcactggca gttgggtcga
     4861 acaccatacc taattctgca ggcgatgcca cccacacgcg ctcaccatct ctgaaaagaa
     4921 cttttcccgt attcgggtaa gaaagtgtct ggctcaactt cacagcagcc tcactgggag
     4981 aaagacccga aaggtccaca ccagcaaccg tcactccagg gaaaatgcgt ccggcgtagg
     5041 caagttgata tccaaccgtc cacacgagaa cgataccaat aaaaagcacg aacccaccgc
     5101 tcaacacggc aagcaactgc ttgggcaaat cacgttgggg aatgaatgcg ttcatggtca
     5161 aagattataa cgaaaaaaat cgggcgatta tgaatagtat ctaagttttg aatgaaaact
     5221 tttcggtaca attgagaaaa tttatttgga t
//
LOCUS       DAOQJE010000123         5232 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_9197_length_5232_cov_5.046938, whole genome shotgun sequence.
ACCESSION   DAOQJE010000123 DAOQJE010000000
VERSION     DAOQJE010000123.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 5232)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5232)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5232
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9197_length_5232_cov_5.046938"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..762)
                     /locus_tag="PLT08_04670"
     CDS             complement(<1..762)
                     /locus_tag="PLT08_04670"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNE03786.1"
                     /translation="MDRTRAILRKAAWSVFTIFFVIILNFFLFRILPGDPARAGIRDP
                     RLNKDAVEALRVRFGLDKPIINCFESLNPIKIGSCSVNPLDTQFFIYVKNLLQGEMGF
                     SYHTNRPVVEVLSERLWNTILLIGVGQILAILFGVAFGIFAAWRARTSIDYASVGASL
                     VAWSLPTFWLAIVLLFWGSMIGLPVAGKATPGMSSYPLLQQWGDIAKHMLLPSITYTI
                     VYMGEYTLIMRSSLLDVLSEDYILTAKAKGLSTVQI"
     gene            complement(835..2469)
                     /locus_tag="PLT08_04675"
     CDS             complement(835..2469)
                     /locus_tag="PLT08_04675"
                     /inference="COORDINATES: protein motif:HMM:NF012706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HNE03787.1"
                     /translation="MKQKVLYLLTLLALVLSACTGGAANQPAIARVGWAGSPDTLNPG
                     MAILTEAYTMFELVYDSMYDLNLDGSFKLSLAESATVSDDGIVWTFKIRDGIKWHDGQ
                     PLTAEDIAWTYNLYKDTPEYPYLNGYYTTYFDTIEATDNNEVVLTLTEAIPNIESQLV
                     FLYILPKHIWENVDKLEYTNTEMIGSGPFKMTEYVQNEFVHLTANKDYFGGAPKIDEV
                     IFQTFENQDALVQAIKTGQVDMITEMPNTAAESLKSEKDIELVVGAPLAPGVTDIIFN
                     QVDPENCPAEGGLCTGHPALRDRNVRLALAHATDKQNLIDVILLGLGKPGLTLIPDGL
                     GVWYNDSITDYEFDVAKANQILDDAGYIDADGDGIRDMPDGSRPLTFRLNWPSDSIPA
                     PRMAELLAEMWGKVGISLEPQAVDPDALTAQCCPAFDFDIMIWGWASDPDPSSLLYVY
                     TSEGIPTGSSETGYSNPEYDALYAKQQVELNFDARKQLVWDMQKIVHDDVVYIIPFYD
                     ANVQAYRTDRFTGWITDQAKVELSDVTSLTVIEPVK"
     gene            complement(2627..3610)
                     /locus_tag="PLT08_04680"
     CDS             complement(2627..3610)
                     /locus_tag="PLT08_04680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016290536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNE03788.1"
                     /translation="MSEPLVRVENLTKHFPIRKGMLIERVVGAVRAVDNISFTIARGE
                     TLGMVGESGCGKTTAGRTLLGLYPATSGKTIIDGHSVHEARGSELMKVRRKAQMIFQD
                     PYASLNPRWTVSSIISEPLRVHGLLSSQKARDERVKELLELVGLNARHINRFPHEFSG
                     GQRQRIGIARALAPDPLFIVCDEPISALDVSIQAQVVNLLEKLQDQLGLTYLFVAHDL
                     SMVRHICDRVSVMYLGVMVELAERDELYENPQHPYTKALLSAVPVPDPRKERERKRVI
                     LSGDVPSPINPPSGCRFHPRCPIAVDRCKAEVPEWREIKPGHHVACHLVEK"
     gene            complement(3607..4602)
                     /locus_tag="PLT08_04685"
     CDS             complement(3607..4602)
                     /locus_tag="PLT08_04685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNE03789.1"
                     /translation="MKTDHKFLEVKDLKTQFNTPEGTVYAVNGVSFSLNEGETLAVVG
                     ESGCGKSVTMMSIMKLIQMPPGKIVGGEVIFRDSDLLKLSEEAMEQIRGREIAMIFQD
                     PMTSLNPVLTVGRQITETLRTHTPMTPEQCRDRAAQLLELVGIPDPVKRLDDYPHQFS
                     GGMRQRVMIAMALACTPSLLIADEPTTALDVTIQAQIVELVKDLKKSVGMAMIWITHD
                     LGVVAGMADRVLVMYAGYVVEEAPVDDLYENTLHPYTLALLQALPRADRRRDQSLKSI
                     KGSPPNLLMEPKGCPFAPRCEFAQEKCIHENPTLAEFEPNHKVACWVDVKTGALR"
     gene            complement(4609..>5232)
                     /locus_tag="PLT08_04690"
     CDS             complement(4609..>5232)
                     /locus_tag="PLT08_04690"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNE03790.1"
                     /translation="AFVEVMMGILIGAIAGYFGGKIDAFLMRFTEAMLNLPQLLLLIV
                     MAKFFSSDIKNINLFGREFSGSVVVIIFIIGLTSWPYLARIVRAEFLSIKEREFILAA
                     RATGTPTWEIIFKHILPNSIAPIVVSATLGVAGAISLEAYVSFLGLGVRAPTATWGNM
                     LENAYRYIESAYWMWLFPGLFIVLIVLSINFLGDGLRDALDPRSRTV"
BASE COUNT         1413 a         1304 c         1214 g         1301 t
ORIGIN      
        1 aatttgaacc gtgctcaatc ctttggcttt ggcggtcaga atgtaatctt cagaaagcac
       61 atctaacaag ctagaccgca taatcagggt gtattcgccc atgtatacga tcgtatatgt
      121 aatagaaggc agaagcatat gctttgcaat atcgccccat tgttgcaata gcggatacga
      181 actcataccg ggcgtggctt tacccgcaac aggtaagccg atcatactgc cccagaagag
      241 caaaacgata gcaagccaaa aggtcggcaa actccacgcg accaaacttg cgccgacgga
      301 agcataatct atggaagtgc gcgcacgcca agcagcaaaa ataccgaatg ccaccccaaa
      361 aaggatagca aggatttgtc ctactccaat taacaaaatc gtgttccaca atcgttcgct
      421 aaggacttct acaacgggac ggttcgtgtg atagctaaat cccatttcgc cttgtagaag
      481 atttttcaca taaatgaaga attgtgtatc gagcggatta accgagcagc tcccgatctt
      541 gatcggattg agcgactcaa agcaattgat aattggttta tctaacccaa agcgaacgcg
      601 caacgcttcc accgcatctt tgttcaaacg cgggtcgcgg attcccgctc gagcggggtc
      661 accgggcagg atacgaaata gaaaaaaatt caaaataatg acaaagaata ttgtgaaaac
      721 agaccaagct gcttttcgga gaatggcgcg agtgcgatcc aagggttttg tctcttgaac
      781 gttgcccaac aggttcaatc aacctgttgg gcaacgtctt tcatttcaaa gtttttactt
      841 cacaggctca ataacggtca aagatgtgac atctgagagt tccactttgg cttggtcggt
      901 aatccatcca gtgaaacggt ctgtgcggta agcttgaaca ttggcgtcgt agaatggaat
      961 gatgtacaca acatcgtcat ggacaatctt ttgcatatcc catacaagct gtttgcgggc
     1021 gtcaaagttc aactccactt gctgtttggc gtagagggca tcatattcag ggttggagta
     1081 gcctgtttcg ctggaacctg tcggaatgcc ttcagacgtg taaacatata acaaagagct
     1141 tgggtcaggg tcagatgccc aaccccagat cataatgtcg aaatcgaatg cagggcagca
     1201 ctgggcagtc aaagcatcgg gatccacggc ttgcggttcg aggctaatac caactttgcc
     1261 ccacatttcg gcgagaagct ccgccatgcg tggagcgggg atactgtcgc tgggccagtt
     1321 caagcggaag gtcaaggggc gtgagccgtc tggcatgtca cgaatcccat ctccatcggc
     1381 atcgatgtaa ccagcgtcat caaggatctg gtttgctttg gcaacatcga attcatagtc
     1441 tgtaatagag tcgttgtacc aaacgcccaa accatcagga atcaacgtga gaccgggttt
     1501 tccaagcccc aacaagatga cgtcaatgag attctgcttg tctgtggcgt gtgcgagcgc
     1561 aaggcggacg ttgcggtcac gcaaggcagg gtgacccgtg caaagcccac cttctgcagg
     1621 acagttttca gggtcaactt gattgaaaat aatatcggtt acacccgggg caagcggcgc
     1681 gccgacaacc aattcgatat ctttttcaga tttcaacgat tcggcagcag tattcggcat
     1741 ttcagtgatc atgtccactt gaccggtttt gatcgcctga accaacgcat cttgattttc
     1801 aaaggtctgg aagatgacct cgtcaatttt cggagcgcca ccgaaatagt ctttgttggc
     1861 tgttagatgc acaaattcat tttgtacata ctccgtcatc ttgaacggac ccgagccgat
     1921 catttctgta ttggtgtatt ccagtttatc aacattctcc caaatatgct tcggaaggat
     1981 atacaggaat accaactggc tctcaatgtt cggaatggct tcagtcaggg tcagaacgac
     2041 ttcattgtta tcggtcgctt cgatggtatc aaagtaggtg gtgtaatacc cgttcaagta
     2101 gggatattct ggggtgtctt tgtagagatt ataggtccag gcaatatctt ctgctgtcaa
     2161 aggctgacca tcgtgccatt tgattccatc gcggattttg aacgtccata cgataccatc
     2221 atctgatacg gtggcgctct ccgccaagct aagtttgaac gagccgtcta aattaaggtc
     2281 atacattgaa tcgtagacca actcgaacat ggtgtacgcc tcggtcaaga ttgccatgcc
     2341 ggggttgagc gtatctgggc tgccagccca gcctacacgg gcaatggcgg gttgattcgc
     2401 tgcaccgcca gtacaagccg aaagaaccaa ggcaagtaag gtgagcaaat acaatacttt
     2461 ctgtttcata acttctcctc cgatgactga ttagcttcta taaaataaaa tgcgccattg
     2521 cagaaaacac ggcaaggcgc attaattaac tggtttctca gggatgaggt tgggctcctt
     2581 cataacctga gctaaatttt actgtagttt acagattacc gaaagttcat ttctctacta
     2641 aatgacaggc aacatgatgc ccaggcttga tttctcgcca ttcgggcacc tctgctttac
     2701 agcggtccac ggcaatgggg caacgcgggt ggaaacggca gccagaaggc gggttgattg
     2761 ggctgggcac atctccgctt aggatgacgc gctttcgctc cctttctttg cgcgggtctg
     2821 gcacaggaac ggcggaaagc aaagcctttg tataggggtg ttgtggattt tcatatagtt
     2881 catctcgttc agccaattcc accatcacac ccaaatacat tactgataca cggtcacaga
     2941 tatgacggac catgctgagg tcatgagcta caaagaggta tgtcagtcca agttgatctt
     3001 gaagtttctc caacaagtta acgacttgtg cctgaataga tacatccaaa gccgaaatcg
     3061 gctcatcgca gacgatgaac agcgggtctg gagccagggc tcgagcgatg ccgatgcgct
     3121 gccgttgtcc gccagaaaac tcgtgcggga agcgattgat atgccgagcg ttcaaaccga
     3181 ccaattccaa aagctctttt actcgttcat ctctcgcttt ttggcttgaa agcaagccat
     3241 gtactcgcaa aggctcactg atgatcgaac ttaccgtcca acgagggttc agggatgcgt
     3301 aggggtcttg aaagatcatc tgcgctttgc ggcggacttt catcagttca ctaccacggg
     3361 cttcatgcac actatgccca tcaataatgg ttttgccaga agtagcaggg tataacccaa
     3421 gcaaagtacg cccggcggtt gtcttaccgc agccactttc gccaaccatg cccaaggttt
     3481 caccgcgcgc aatggtgaat gaaatattgt ccacagctcg tacagcgcca accacacgtt
     3541 cgatcaacat cccttttcga attgggaaat gcttggtcag gttttcaact cgtaccaacg
     3601 gttcgctcat cgcaatgctc cagtctttac atctacccag caggcgactt tatggtttgg
     3661 ttcaaattca gctaatgtag ggttttcatg gatgcatttt tcttgagcga attcacagcg
     3721 tggcgcgaag ggacatcctt taggttccat caacagattt ggcggagagc ccttgataga
     3781 tttcaagctt tggtcgcgtc ggcgatctgc tcgtggtaaa gcttgcagta atgccaaggt
     3841 ataaggatgc aatgtatttt catacaggtc atccacagga gcttcttcca ctacatatcc
     3901 ggcatacatc acaagcacac gatctgccat acctgccaca acacctaaat catgcgtgat
     3961 ccaaatcatg gccatgccca cgcttttctt taggtctttc accaattcaa cgatctgcgc
     4021 ctgaattgtc acatccaaag ctgtggtcgg ttcatctgcg atcaacaaac tgggcgtgca
     4081 agccaacgcc atagcaatca tcacacgctg gcgcatgcca ccagaaaact gatgtggata
     4141 atcatccaac cgtttgactg ggtctggaat cccaacaagt tctaataact gagcagctcg
     4201 atctcgacac tgttcaggcg tcataggagt atgagtgcgc agcgtttctg tgatttgacg
     4261 acccacggtc aacacaggat ttagactggt cattgggtct tggaagatca tcgcaatctc
     4321 acgcccgcgg atttgttcca tcgcctcttc acttaatttg agcaggtcgc tgtctcgaaa
     4381 gatcacttcc ccgccgacaa ttttcccggg tggcatctga attaatttca tgatggacat
     4441 catcgtcacc gatttgccac aaccactttc gcctacaaca gctagtgtct caccttcgtt
     4501 caagctaaac gaaacgccat tcacagcata gacagtccct tccggtgtat tgaactgcgt
     4561 cttgaggtct tttacttcaa ggaatttatg gtctgttttc ataagtcatt acaccgttcg
     4621 gctacgaggg tcaagagcat cacgcaagcc atcacccaaa aagttgatgc taagcacgat
     4681 caaaacaata aataaaccgg ggaacagcca catccaatac gcagactcaa tgtagcgata
     4741 ggcgttttcg agcatattgc cccaagtcgc tgtcggcgca cgaacgccta agcctaaaaa
     4801 gctgacatat gcttcaaggg agattgcgcc cgcaacgccc aaggtagcgg atacgacgat
     4861 tggagcaatg ctattcggca gaatatgctt gaaaataatc tcccaagttg gggttccagt
     4921 tgcgcgtgcc gcaagaataa actcacgttc tttgatagac agaaattcag cacggacaat
     4981 acgcgccaaa tatggccaac tcgtaagacc aatgatgaag ataattacga ccacactgcc
     5041 gctaaactcc cttccaaaga gattaatatt tttaatatca ctggaaaaga actttgccat
     5101 tacgatcaac aaaagcaatt gcggcaaatt cagcatggct tcggtaaagc gcatcagaaa
     5161 tgcatcgatc ttcccaccaa aatagccagc aatcgcccca atgagaattc ccatcatcac
     5221 ttctacaaaa gc
//
LOCUS       DAOQJE010000124         5228 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_9217_length_5228_cov_5.042915, whole genome shotgun sequence.
ACCESSION   DAOQJE010000124 DAOQJE010000000
VERSION     DAOQJE010000124.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 5228)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5228)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5228
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9217_length_5228_cov_5.042915"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(283..744)
                     /locus_tag="PLT08_04695"
     CDS             complement(283..744)
                     /locus_tag="PLT08_04695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE03791.1"
                     /translation="MVENATNSVVNEITNQIEAEKPSKLTINQLQISFSGGSFRRNVP
                     NIRLNILSQIHHGIIQVTVSKNKLIVFYRLWYTEMFVIVTVVSILFALLLIISSGFTI
                     QSLWVALIIWAFLYLGNYVISVIRFNIFIHFAINKLDFITDNEYKIFQENE"
     gene            complement(884..2035)
                     /locus_tag="PLT08_04700"
     CDS             complement(884..2035)
                     /locus_tag="PLT08_04700"
                     /inference="COORDINATES: protein motif:HMM:NF014686.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HNE03792.1"
                     /translation="MSTILLTGGRAPVTLDLARAFHRAGHVVYVAESLRGHLTEPSAA
                     IKAHFEVPAPRQEPEAFLAALRSIIEAYQIELLIPTCEEVFHIAKGLNTLPCRVFTEP
                     LARLDGYHNKWKFVVKAGECDLRVPESMLVTRQDDLLYAYAHWRGLVLKPVYSRFAAR
                     TLILPPLKDAMATLTFDSLTNQEPWIAQEYIEGEQFCSYSVCQNGRILAHAVYPTAFT
                     AGQGATIAFQSVQHDAIYEWVRTFVEHYKVTGQMAFDFVQTADGNVVALECNPRATSG
                     AHLLTSNPRFVESFFNSELECITPYEGTSRMLGTAMLTYGLPASLFNGRFGAWLKTFS
                     HSDDVLLDFRDPLPFLLQWRSILSYVLLGRKQGISALEASTFDIEWNGE"
     gene            complement(2063..3034)
                     /locus_tag="PLT08_04705"
     CDS             complement(2063..3034)
                     /locus_tag="PLT08_04705"
                     /EC_number="3.4.13.19"
                     /inference="COORDINATES: protein motif:HMM:NF013413.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070573 - metallodipeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase"
                     /protein_id="HNE03793.1"
                     /translation="MKTQTTDIHRQSIVWDTHACFPLNPQSDLSELKRYKDSGVTFVS
                     LNIGMDMDSFENVMRVLARFRSHIAAHPNELILARSVEDIHRAKRENKLAIAFDLEGS
                     NPLCGDLNLISFYYDLGVRQMLLAYNKDNAASGGCMEGRIGLTEFGRQVVQEMNRVGM
                     VVDVTHMGYRATMDTFAVSTKPVIFSHSNPARLHAHARNISDEQIKACAKTGGVIAIN
                     GIGDFLGGKTSELFVQHLEYVLNLVGPEHVGIGLDYVIDKQELIDYIQGNPDVFPADR
                     FEDFLSFVEPEQLPEISEILYKHGHDEDVIRKVLGGNFLRVAEQTWK"
     assembly_gap    3242..3341
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<3342..4228)
                     /locus_tag="PLT08_04710"
     CDS             complement(<3342..4228)
                     /locus_tag="PLT08_04710"
                     /inference="COORDINATES: protein motif:HMM:NF012336.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] synthase III
                     C-terminal domain-containing protein"
                     /protein_id="HNE03794.1"
                     /translation="MQVNIPLKIIGLGRYLPKRVVPSSELEALCGVPAGWVERRNGVR
                     ERRWVTDETSSFMSAEAGREALEEAKLKPNQLDLIINASGTGEQAIPDTGVLIQRQLG
                     LGASGIPAMTVHTTCLSFVAAMDVASNFIQSGRYKNILIASADVASCGINPKEPESAS
                     LVGDAAAAVVVTRANDGDASRIHHAHFKSFGDGAYLTTIMGGGSRFHPRFEGHKPEDD
                     LFHMDGPAVLRMVRGIAHEFLDELYPGLSKSMVDVDVVVPHQASKVGLMMLERFGWKD
                     EKIIKTIETLGNCVAASIPV"
     gene            4364..>5228
                     /locus_tag="PLT08_04715"
     CDS             4364..>5228
                     /locus_tag="PLT08_04715"
                     /inference="COORDINATES: protein motif:HMM:NF016090.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="circularly permuted type 2 ATP-grasp protein"
                     /protein_id="HNE03795.1"
                     /translation="MTPETQYSMPESVLSNLYDPAPFYDEMFSSAGQPRPHYEVLRKY
                     LQGMTAEMFAERRRAADAAFLYQGITFTVYGQEDGIERIFPFDLVPRIIPNTEWDHLE
                     RGLTQRVTALNLFLHDIYHEQRILREKRIPPELVFSSRNFRHEMIGVNVPKDRYVHIV
                     GTDIVRGGDGEYYVLEDNLRSPSGVSYMLENRQAMKQTFADLFSQYGVLPIEHYPQEL
                     LNTLRSVAPHDYSDATVVLLTPGIYNSAYFEHTFLARQMGIEIVEGRDLVAHQNKIYM
                     RTTRGLKLVDVI"
BASE COUNT         1337 a         1409 c         1119 g         1263 t
ORIGIN      
        1 tcagagaagc tgacttttgc cgctgcttct ggagcatcca tcgtagtgca tcgagcgctg
       61 gatgtaacca atagaagagc aactacaacc attctccaag atgccttcat ggctttacct
      121 cttcagagcg attcaattat tatgtttgtt tcaaacataa tactcttttc ttaaattata
      181 cgtcccccat ctaaaaaaac aactaccccc ctcgcaaccc tcatacgttg acctagccaa
      241 gtccagccag aaaaggttat gccactttgg cttagtttaa gtttactcat tctcttgaaa
      301 aattttatat tcattgtctg taataaaatc tagcttgttt atagcgaaat gtatgaatat
      361 attgaagcgg ataactgaaa taacatagtt acctagatat aaaaatgccc atattattaa
      421 tgccacccat aaactttgaa ttgtaaaacc tgaggagatt ataagaagta gtgcaaacaa
      481 aatggatact actgtcacta taacaaacat ttctgtatac cagagtctgt aaaaaactat
      541 cagtttgttt ttgctgactg taacttgaat gattccatga tgtatttgag acaatatatt
      601 caaccgaata tttggaacat ttcttcggaa agagccacct gaaaaagaaa tctgaagttg
      661 gttgattgtc agtttactcg gcttttctgc ctcgatttgg ttggtaattt catttacaac
      721 gctgtttgta gcgttttcaa ccatactgtc tgtgataatg aattcaattt tttggctaaa
      781 cgtaaagggt atcattattt accatacttg ttttacaaag caaaaacgaa cggaattgcc
      841 cttcccaacc ctcacgtgtt gatctagcca attccagaaa accctactcc ccattccatt
      901 caatatcaaa agtggatgcc tctaatgctg agatgccttg cttgcgcccc agcaacacat
      961 atgagaggat gctgcgccat tgcagaagga agggcaatgg gtcgcgaaag tctaagagca
     1021 catcgtcgct gtgagagaag gtttttagcc acgccccaaa gcgaccgtta aatagcgagg
     1081 cgggcagccc ataggtcaac atggctgtgc ccaacatacg cgaagtccct tcgtagggtg
     1141 taatgcactc cagttcactg ttaaagaaag actctacaaa gcgcgggttc gaggtcagca
     1201 aatgcgctcc gcttgtggca cgtgggttac attccaacgc caccacgttg ccatctgctg
     1261 tttgcacaaa atcgaacgcc atttgcccgg tcactttgta atgctccaca aaggtccgca
     1321 cccattcata gatggcatca tgctgcaccg attgaaacgc aatcgttgct ccctgcccgg
     1381 ctgtgaacgc cgtgggatag accgcatgcg ccagaatgcg tccattctga caaaccgaat
     1441 aactacaaaa ttgctctccc tcaatatatt cttgtgcgat ccacggctct tggttcgtga
     1501 gagagtcaaa cgtcagcgta gccatggcat cttttaacgg gggcaagatc aaggtccgcg
     1561 cagcgaagcg ggaatagacc ggcttcagca ccagtccgcg ccagtgagcg taggcataga
     1621 gcaagtcatc ttgtctcgtc accaacatcg actcaggcac acgcaaatca cattcccccg
     1681 ccttcaccac aaacttccac ttgttgtgat atccatccaa ccttgccagc ggctcggtaa
     1741 acacgcggca gggtaaagta ttcaaccctt ttgcaatatg gaaaacttcc tcacaggtgg
     1801 ggatcagcaa ctcgatctga tacgcttcga tgatgcttcg caacgctgct aaaaacgcct
     1861 caggttcctg acgtggagct ggcacctcaa aatgagcctt gatcgccgcc gaaggctcgg
     1921 tgagatgccc gcgtaaagac tcggcaacat acactacatg ccccgctcgg tgaaacgcac
     1981 gcgccagatc taaggtgacg ggcgctcgcc cgccggtgag aagaatggtt gacattaggt
     2041 cagcctgcca ataagggatt ttttacttcc aggtttgttc tgccacccgc aagaagttcc
     2101 cacccaacac cttacggatc acatcttcat catgcccgtg cttgtataaa atctcgctga
     2161 tctctggcaa ttgttctggt tctacgaacg aaaggaaatc ttcaaagcgg tcggctggga
     2221 agacatccgg atttccctga atgtagtcga taagttcctg tttgtcgatg acataatcca
     2281 atccaattcc cacatgttcg ggtccgacca aattcaaaac atactcaagg tgttgtacaa
     2341 aaagctcgct ggttttgccg cctaaaaaat cgccaatgcc gttgatggcg atcacgccgc
     2401 cggtcttggc acatgctttg atctgctcat ctgaaatatt tcgggcatgg gcgtgcaaac
     2461 gggcagggtt tgaatgtgaa aaaatgaccg gcttcgtaga gactgcaaag gtatccattg
     2521 tggcgcggta gcccatatgc gttacatcca ccaccatgcc cacacggttc atctcttgca
     2581 ccacttgcct gccgaactcc gtcagcccga ttctcccctc catgcacccg cctgaagcgg
     2641 cattgtcttt gttgtatgcc aatagcattt ggcgcacacc aaggtcataa tagaacgaga
     2701 tcaggttcaa gtcgccgcat aatgggttcg acccttccag atcaaaagcg atggctaatt
     2761 tattctctcg tttggcgcgg tgaatatctt ccacagaccg tgccagaatc aattcattgg
     2821 gatgagccgc aatatgggag cgaaaacgtg ccaacacgcg catcacattc tcgaacgagt
     2881 ccatgtccat gccgatgttg agcgacacga aggtcacgcc gctgtctttg tagcgtttca
     2941 gctcgctcag atccgattgc ggattcaatg gaaaacaggc atgcgtatcc catacgattg
     3001 actgacggtg aatgtctgtg gtttgggttt tcattatgta tataccgtat gggcggggtc
     3061 attccgccta tacaaaatta aaacgtcaac accataccgc caatagacaa gcccgcgccc
     3121 gtgcccacca acaacacctt atcgccgcgt tgaatgcgtc catcgcgcac gccttgatac
     3181 aaagttacag ggatcgaagc cgccacacaa ttaccgagcg tctcaattgt cttgatgatc
     3241 tnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3301 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nacagggatc gaagccgcca
     3361 cacaattacc gagcgtctca attgtcttga tgatcttttc atccttccaa ccaaagcgtt
     3421 cgagcatcat caaacccacc ttgctggctt gatgcggcac gaccacatcc acgtccacca
     3481 tgctcttgga aagacccggg tacaactcat ctaaaaattc atgggcgatt ccacgcacca
     3541 tgcgcaacac tgccggccca tccatgtgga acaaatcatc ttctggctta tgtccttcaa
     3601 agcgcggatg aaagcgcgag ccgccgccca tgatcgtcgt cagataggcg ccatcgccaa
     3661 acgatttgaa atgtgcatga tggattctcg aagcatcgcc gtcattggca cgcgtcacca
     3721 ccacagccgc cgcggcatct cccaccaacg aagccgactc aggttccttc gggttgatcc
     3781 cgcacgaagc cacatctgca ctggcgatca gaatattttt ataccgtccg ctttggatga
     3841 agttcgacgc cacatccatt gccgccacaa aacttaaaca ggttgtatgc accgtcatcg
     3901 ccggaatgcc tgaagcgcct aaacctaatt ggcgttggat caacaccccc gtatccggaa
     3961 tcgcctgctc tcccgttccc gaagcgttga tgatcaagtc gagttgattc ggcttgagct
     4021 tcgcctcctc caatgcctct cgccccgcct ccgccgacat aaatgatgac gtttcatccg
     4081 tcacccaacg gcgttcacgc acaccattgc gtcgctccac ccaaccagcg ggcacaccgc
     4141 acagcgcttc gagctctgag ctgggcacaa ctcgtttcgg taaatatcgt ccaagaccta
     4201 taattttgag ggggatgttg acttgcatag aagagactcc aataaggaat gattttatga
     4261 gattatctca tacccgcaaa aaatcaacca taagggataa caagaatact acccataagg
     4321 gtactctcgg ctttctgcct tccataagta tttcatggta aaaatgaccc cagagaccca
     4381 atactcgatg ccggaatccg ttctatcgaa cttgtatgat cccgctccct tctatgatga
     4441 gatgttctca tcagccgggc aaccacgtcc gcactatgaa gtgctgcgca agtatctaca
     4501 agggatgacg gcagagatgt ttgccgaacg ccgccgtgcc gccgatgcgg cgtttttata
     4561 tcagggcatc accttcacag tgtatggtca ggaagatggc attgaacgca tcttcccctt
     4621 cgacctggtg ccgcgcatca tccccaatac agagtgggat cacctcgagc gcggactcac
     4681 ccaacgggtg acggcactca acctcttcct ccacgatatt tatcacgaac aacgcatcct
     4741 gcgcgaaaaa cgcatcccgc ctgaattggt ctttagctcg cgcaattttc gtcatgagat
     4801 gatcggcgtc aacgttccca aagaccgcta tgttcacatt gtagggacgg atattgtccg
     4861 cggcggtgat ggcgaatatt atgtgctcga agataacctg cgctcgccct ctggcgtgag
     4921 ctacatgctc gagaatcgcc aagccatgaa gcaaaccttc gccgacctgt tctcacaata
     4981 tggggtcttg cccatcgagc attatccgca ggaactactc aacacccttc ggtctgttgc
     5041 cccgcatgat tattccgatg ccaccgtggt gttactcaca cccggcattt ataactccgc
     5101 ttattttgaa cataccttcc tggcacgcca aatgggcatt gagatcgtgg aaggacgcga
     5161 cttggttgcc caccaaaaca agatctacat gcgcaccacc cgcggactca aactggtcga
     5221 tgtcatct
//
LOCUS       DAOQJE010000125         5228 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_9218_length_5228_cov_4.886720, whole genome shotgun sequence.
ACCESSION   DAOQJE010000125 DAOQJE010000000
VERSION     DAOQJE010000125.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 5228)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5228)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5228
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9218_length_5228_cov_4.886720"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1036)
                     /locus_tag="PLT08_04720"
     CDS             complement(<1..1036)
                     /locus_tag="PLT08_04720"
                     /inference="COORDINATES: protein motif:HMM:NF012464.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HNE03796.1"
                     /translation="MKNEALRAVGYRRVSMREQVDGFSLDAQENNIRQYVEAQGWQLT
                     HIYTDAGISAKKDSHRPSLEQMMLDAEARRFDVIVVDKVDRFYRHLRGLLSALDQLNS
                     WNVAFASVQERLDFTTVWGKLTLTVLGTLAEIYIDNLRQETKKGKLQRARDGYWNGNP
                     TYGYCRGLCSACKEPNGKGYCPDYGLSNKGDGKGLVLHPVESKVVRLVYDWYLTGEES
                     DGHIAERLRTYGLDEHGLLARSRGIPGQKGPGPIQKDSIRNILTNILYAGFIPYYGTS
                     KKGQTTSRSVKEIFPGKHPAIVSMEEYEHVQEIRNVLYRAPRFKRNTQSRIYPLSRIL
                     RCGYCGRTMRG"
     gene            1121..1543
                     /locus_tag="PLT08_04725"
     CDS             1121..1543
                     /locus_tag="PLT08_04725"
                     /inference="COORDINATES: protein motif:HMM:NF013540.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HNE03797.1"
                     /translation="MADASLAKKRKLSVKKKYDPKPFIHRIEQLMEARNLSMRETALS
                     SGLDHQAIRRIKAGQKRPDMTYCILLANFFNLNPNELLQLGGWPTLKVFDIKQVSAER
                     LPPEAVDVALDIAKIPDPGTRKQVAEAVRILLKKYFGD"
     gene            2380..2880
                     /locus_tag="PLT08_04730"
     CDS             2380..2880
                     /locus_tag="PLT08_04730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE03798.1"
                     /translation="MKLLRFSHPLPWTDLQLYDWLTTTIKGIRFVRGGVSHCCEPKNI
                     VVIALNGDSMLLLTDQWVMDGSIHGLMDATLLYAHEARHNEGFLHACTTKNGNDNTLD
                     EMGAWAISHYFALWIAQYGDRAFLKPQSGSPDLFREAALQQAEITRLTRFCKEVYMEP
                     ATTLAP"
     gene            3152..3736
                     /locus_tag="PLT08_04735"
     CDS             3152..3736
                     /locus_tag="PLT08_04735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE03799.1"
                     /translation="MSKFNFWTYEELLYIQKDLERFDQASKVDLGTLYPSSVKLKRIL
                     ANDNKACAETNRYFVKNSSIVFYNSFDKLCGEGVLVHELGHYLDGINQRKYSKEFEEY
                     VGAFTFLWWYNLGQENPPIYGSGYPPNRREDFAESLEEYVLLYTNYSGPDIGIVPGEK
                     RWKFIEFVLDTGQLSINKEQYLCLPSYGKLINLN"
     gene            3754..4404
                     /locus_tag="PLT08_04740"
     CDS             3754..4404
                     /locus_tag="PLT08_04740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE03800.1"
                     /translation="MKIKTIILRILLIPLPLILVLTGCKDSKGTVLVGTRDCEPPCWL
                     DIMPGVTKSGDMIEILKKKEQAKEGFLTLLDGGIIRWQSLDGYNSYVYPSENEVVTRI
                     EVDLRSNSIKLDDLISIFGEPLTLDIGKVEDGYFRSTVFYPNKGLAFVISGDEIDITT
                     SDLKFLFNPDMAVLKGIFFSPMEDISTMINLLYSSNAKPSVLLNIQNWTGYGIYYK"
     gene            complement(4544..>5228)
                     /locus_tag="PLT08_04745"
     CDS             complement(4544..>5228)
                     /locus_tag="PLT08_04745"
                     /inference="COORDINATES: protein motif:HMM:NF012798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HNE03801.1"
                     /translation="VWFAYVQERIKAGIKPSSLNTELKVVQSLIRFASENNIPVCRMM
                     LEVHPLKTGEALPKDLNMGQVKALLNAARNKTEEAWILLMLHCGLRTCEIRALRWEDV
                     DLQKRVIYIRESKGLQSRAVFLSLPVKEALEGLPQNHGFIFTYRNESLSRRYCQSRLS
                     TLGRQVGFHVTPHQLRHTCATMLLNAGMSILGVQRLLGHKYVETTLRYARLYDVTVVR
                     DFWKVSDVK"
BASE COUNT         1466 a         1160 c         1098 g         1504 t
ORIGIN      
        1 aaccccgcat cgttcgcccg caatacccac atcgtagtat tctagataat ggatatatcc
       61 ttgactgggt attgcgtttg aagcgaggcg cacgatataa gacattacgg atctcttgta
      121 catgttcgta ttcttccatg ctaacaattg cgggatgctt tccaggaaat atctctttca
      181 cacttcgtga tgtcgtttgc ccctttttag acgtaccgta gtacgggata aaacccgcgt
      241 agagaatatt tgtgagaatg ttacgaatcg agtccttctg aattggtcct ggtcctttct
      301 gaccaggaat gccacgagac cgggcaagca aaccatgttc atccaaacca taggttctca
      361 atcgttctgc gatatgcccg tccgactcct cccctgttag ataccaatca tatacaagcc
      421 gaaccacctt gctctcgacc gggtgcaaca ccaacccttt tccatctccc ttattagaca
      481 atccataatc tggacaatat ccttttccat tgggctcttt acaggcagaa cacaacccac
      541 ggcaataacc atacgtggga ttcccattcc aatacccatc gcgggcacgt tgcaacttcc
      601 ccttcttcgt ttcctgacgc agattatcta tatagatctc tgccaaggtg cctaacaccg
      661 tcaatgtcag ttttccccag acagtagtga aatccaaacg ctcctggacg gatgcaaaag
      721 cgacgttcca actattaagc tgatcaagcg cactcagtaa tccacgcaga tgacggtaga
      781 aacggtccac cttatcaaca acaatgacat cgaaacgtct tgcctcggca tctaacatca
      841 tttgttccag tgatgggcgg tggctgtctt tttttgcgga gatgcctgca tcagtatata
      901 tatgtgttaa ctgccagcct tgcgcttcca catattgccg gatgttgttt tcctgcgcat
      961 ccagactgaa gccatccacc tgttcccgca tggacacacg ccgatatccc acagctcgaa
     1021 gtgcttcgtt cttcatctta ccacctccta ttgggtataa tttcctatcg agaccgctgg
     1081 gtctgcattc attttatctt taacacctgg agcacttcgt atggctgatg cgtcacttgc
     1141 gaaaaaaaga aagctttctg tcaaaaaaaa gtacgatccg aaacccttca tccatcggat
     1201 cgagcagtta atggaagcaa gaaatctcag tatgcgcgag actgcattaa gcagcgggtt
     1261 ggatcatcag gcgatccgca ggatcaaggc ggggcaaaaa cgcccggata tgacgtattg
     1321 catcctactg gcaaattttt ttaatctcaa tccaaatgag ttgttacaac ttgggggctg
     1381 gccaaccttg aaagtctttg atatcaagca agtgagcgct gaacgtctcc cgcccgaagc
     1441 agtagatgtg gcattggata ttgcgaagat tcccgaccca ggcacccgca aacaagtcgc
     1501 ggaagcggtg cggatattac taaaaaaata ttttggtgat tgaagaaaaa gaacagactg
     1561 tgcaagcctg ttcttttttt ctatttatct actatcaagc cacccgatgg caagttaagt
     1621 aaacgatcaa taataggtag ggattgcttt agtttgtcct tgcgtcctag tcaggaacct
     1681 gccagtgttg ggatcacacc tgcactgcac cgagtgcaac gagatgcaag tgtaatactg
     1741 gtactctgtt ggggctttta ctaaatctca gccgcacttt ttcacgcagt aagcgattta
     1801 cacattccca aatacagtcg tatcaccaca gacgaaaaca atggtataca ccttttcaag
     1861 gtggtatttc cttcagcaac gatctccatg cgcaaccgcc ttgtagtatg taatcatatg
     1921 ccagtcatgc tcacactgat aatctttttt gcttcggctg gcagatatgg tcaaattttc
     1981 gagcattgtg tatcattcag ttcagttgtg tacgtatgtt gtgatttggt catccaataa
     2041 ttttgcccaa ccatccctat ttttcaaggt ttttaggctc tctcttaata tttacgtatg
     2101 aatttcgaac agttaattac tttaggtttt tcattttttc tattcagttg ttcttccttg
     2161 gaaaggtctg taattaagct caccctaaca ccagacggtg aatctattgg tgaatttttt
     2221 gagcgttgcc ccacacttga agaggttgta caggtcaacg cggatttgag aatcatcgtg
     2281 gaataagacc ccacatctgg tgtggacgtt tgccatgaaa atgacggctc tgcaaattta
     2341 agcgccctgc aaaagcggat ctatcaaacg gtgtatgtta tgaagttgct caggttctcc
     2401 caccctctgc cgtggacaga tttacagtta tatgactggc tgacgacaac tatcaaaggc
     2461 attcggtttg tacgtggggg tgtcagccac tgctgtgagc ctaaaaacat cgttgtcatt
     2521 gcactaaacg gagattcgat gctactcctc accgatcaat gggtaatgga tggcagcata
     2581 catggactaa tggatgcaac cttgctttat gctcatgaag cgagacacaa cgaaggcttt
     2641 ctgcatgcat gtactacaaa aaatggaaat gacaatacgc ttgatgaaat gggagcatgg
     2701 gcaatttcgc actattttgc cttatggatc gcccaatatg gagaccgcgc ctttcttaaa
     2761 ccccaaagcg gatcaccgga tttgtttcgg gaagccgcct tgcagcaagc agaaatcacc
     2821 cggctcacaa gattttgtaa ggaggtatac atggagccgg caacaacttt agcaccctaa
     2881 tcgtgaacaa taaggggatt ccacacagtt aatttacctc aggtccagat attacaaccc
     2941 cgccgatggg aggttccagt cacgggatac gtggagtggg gattacaacc gtcccttgtc
     3001 gctgaaccgg tggatgtacg tcgaggggaa tcctgtcaag tatacggatc caagtggatt
     3061 ctgccctgaa cctcgaacag atgaagaccc atgctggggt cttctacttc aaattgaatc
     3121 tcgttatagt tttgtggatt tagagtctga gttgagtaaa tttaattttt ggacttacga
     3181 agaattatta tatatacaaa aagacctcga aagatttgac caagcttcta aagttgactt
     3241 aggcacactt tatccctcta gcgtaaaatt aaagagaatt ttggctaatg acaataaggc
     3301 ttgtgcagaa acgaatcgct attttgtaaa aaacagctcc attgtttttt ataacagctt
     3361 tgataaattg tgtggggaag gggttttggt gcatgaatta gggcattatc ttgatggtat
     3421 aaatcagcga aagtatagta aagaattcga ggaatatgtt ggggcattca cttttctttg
     3481 gtggtacaac cttggacaag agaacccacc aatttatggc tcaggttatc ctcctaatcg
     3541 tcgcgaagat ttcgccgaat cactagaaga atatgtttta ctttacacaa attattcagg
     3601 accagacatt ggcatagttc ctggcgaaaa aagatggaaa tttattgagt ttgtcttaga
     3661 cacaggacaa ttatctataa ataaagagca atacctctgt cttcccagct atggtaaatt
     3721 gataaatctc aattagagat tcaatggtga gttatgaaga ttaaaacaat tattttgcgc
     3781 atattgctaa ttccacttcc tttgattttg gtactaacag gttgcaaaga ttctaaagga
     3841 actgttttag ttggtacaag agattgcgag ccaccttgct ggttagatat tatgccagga
     3901 gttacaaaaa gtggtgatat gatagaaatt cttaagaaga aagaacaggc aaaagaagga
     3961 tttttaacac ttttggatgg tggaattata aggtggcaat cactcgatgg ttacaactct
     4021 tatgtttacc cgtcagagaa cgaagttgtt accagaatcg aggttgactt gcgatctaat
     4081 tcaataaagt tggatgattt aattagcatt tttggagagc ctttaacatt agatattgga
     4141 aaagttgaag atggatattt tcgttctact gtattttatc caaataaggg attagcattc
     4201 gttattagtg gtgatgaaat tgatattacc acttcggatt tgaagtttct tttcaacccc
     4261 gatatggcgg ttttaaaagg tatttttttt agtccaatgg aagatatatc tactatgatc
     4321 aatttacttt attcgtcaaa tgccaaaccc agcgtattgt taaatatcca aaattggaca
     4381 ggatatggga tttattataa ataatattgg aactgaaaga tgtatggcaa catcctacgt
     4441 ataaattttc aggaaattac catacatcct aaaacttact tgcttatcaa tacccacact
     4501 ccaacgtcag aaagcacatc gtcaacgact agagtcggtt tctttatttc acatcactta
     4561 ccttccaaaa atctctcaca actgtgacat cgtacaacct tgcatatcgc aaggtcgtct
     4621 ccacatattt gtgtcccaac aacctctgca ctcccagaat ggacatcccc gcattgagga
     4681 gcatcgtcgc gcaggtgtgc ctgagttgat gaggtgtgac atgaaacccg acttgcctgc
     4741 cgagggttga caacctgctc tgacaatagc gacgactcaa agactcgtta cgataggtga
     4801 atataaatcc atgattttgc gggagtccct ccagtgcttc ctttactggt aggctcagaa
     4861 aaacagcgcg actttgtaag cccttagatt ctcggatgta gataacccgc ttttgtagat
     4921 caacatcctc ccagcgcaag gcgcggattt cacaagttcg taacccacaa tggagcatga
     4981 gtaatatcca ggcttcctca gttttgttac gagcggcatt tagcaaagct ttgacctgac
     5041 ccatgttcaa atccttgggt agagcctccc ccgtcttcaa gggatgtacc tccagcatca
     5101 tcctgcaaac agggatgttg ttctcacttg caaatcgaat cagggactga acgactttca
     5161 attctgtgtt cagcgaggaa ggtttgatgc ctgcttttat cctctcctga acgtaggcaa
     5221 accagacc
//
LOCUS       DAOQJE010000126         5217 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_9250_length_5217_cov_6.179388, whole genome shotgun sequence.
ACCESSION   DAOQJE010000126 DAOQJE010000000
VERSION     DAOQJE010000126.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 5217)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5217)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5217
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9250_length_5217_cov_6.179388"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..378)
                     /locus_tag="PLT08_04750"
     CDS             complement(<1..378)
                     /locus_tag="PLT08_04750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE03802.1"
                     /translation="MSLDSTSPRTYRFVLADILTSGYRVAGKVAVTTHGAMGFLNDIN
                     HSTLEIHDARMARLHMPTKLVDHFELVRTMKSRIHAVCLPRREDLGPQGIVRGGYMNV
                     VEYQVRLTTSMFEVDGIMELPGRF"
     gene            complement(393..680)
                     /locus_tag="PLT08_04755"
     CDS             complement(393..680)
                     /locus_tag="PLT08_04755"
                     /inference="COORDINATES: protein motif:HMM:TIGR03831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgiT-type zinc finger protein"
                     /protein_id="HNE03803.1"
                     /translation="MNELNALTPCNECHAGLLHPRHVTYFTWLGHELITVPHFPAWVC
                     DVCGRREYDERAIQWLNMILDPNAGKPTPSHRNTPPRPRPQTGFRSPTPES"
     gene            complement(689..1276)
                     /gene="lepB"
                     /locus_tag="PLT08_04760"
     CDS             complement(689..1276)
                     /gene="lepB"
                     /locus_tag="PLT08_04760"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: protein motif:HMM:TIGR02227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HNE03804.1"
                     /translation="MDTLEQEPLVEESKPAEETTNWKRFVLDVIETLVLAVILFVGIN
                     SVSARVRVDGFSMQPTLQDGEFVLVSKLSYKFGDFQRGDIIVFDFPMNPDEELVKRII
                     GVPGDHVMVRDSHVYVNEQMLNENYIAQAPLYSGDWIVPDGTLFVLGDNRNNSNDSKD
                     WGLLPQGNVVGKAVLIYWPPKFWKVLDHPEMAVSQ"
     gene            complement(1476..2039)
                     /locus_tag="PLT08_04765"
     CDS             complement(1476..2039)
                     /locus_tag="PLT08_04765"
                     /inference="COORDINATES: protein motif:HMM:NF012330.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016311 - dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dual specificity protein phosphatase family
                     protein"
                     /protein_id="HNE03805.1"
                     /translation="MKHLPIIESYWVEEGHFLAGEYPGSYNPEDARRRMDAFLESGIR
                     TFIDLTQPHELVPYEPILKEEARIYEHMAVYHRFAIRDHGIPSVQTMTLILDTIDAAI
                     ESGSPVYVHCWGGVGRTGTVVGCYMVRHGLTPSQALERVNALYKTRPQNYFLVRSPET
                     EEQADFVRKWGETPGFGHKAKQRFCEG"
     gene            complement(2043..3077)
                     /locus_tag="PLT08_04770"
     CDS             complement(2043..3077)
                     /locus_tag="PLT08_04770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE03806.1"
                     /translation="MPRLKFLFALAALILSSLACVTLFGQKSASAGIAPGMAIPATAT
                     PEALSCPLITNKIVDQNATIDLSNQLPLDVLNSSVDDGDEAIYLVTYTVDGNEIRDPY
                     FEQDVPADLVDEQNDTALHQQLWEYFSTVIPLEARPTLAEFSIMTDGSDNVLAAVAQT
                     YDDPNRWSLEVDVADTSDYYYLTFTLVHEYAHLLTLGPDQVPPSLAVFNNPDDNDLYL
                     QEVAACATFFPGEGCSNPDSYMNAFYDQFWTNIYDEWNQINLEEDEDLYYEKLDDFYS
                     KYEDQFLTDYSATNPAEDIAESFGFFVFADKPDGDRIAEQKILFFYQYPELVELRTKI
                     LNNVCVNFPQ"
     gene            complement(3171..4160)
                     /locus_tag="PLT08_04775"
     CDS             complement(3171..4160)
                     /locus_tag="PLT08_04775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE03807.1"
                     /translation="MSKNISFMGVLLSLVLFLFIGLYFGQKSILPLGPDAYASCAVSM
                     DHVLDSGKGDVYNFSGVTNFEEPAFYYLASYDVEESTITDPILKTVPKDLQDEQHDTV
                     LQQEAWSIFTTLIPVQNRQMVSQFNVFTDGYSNTLAAVDQTENDLSQWMLEVDVADLE
                     DKDALIYTMVHEYAHLLTLNSSQVTPDADTYKDPLNPELQKEKAAACSTYFVGTGCTH
                     PDSYMNAFYNRFWADISDEWQQVDALQYGAEDLVPYYNGLYAFYLKHKDRFVDDYAVT
                     HPAEDIAESFTYFVFNPKPVGNSIRDQKLAFFYEYPELVQLREDILSGACSMK"
     gene            complement(4182..>5217)
                     /locus_tag="PLT08_04780"
     CDS             complement(4182..>5217)
                     /locus_tag="PLT08_04780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE03808.1"
                     /translation="LLCYTYGMEICYLIAFPDTEHKVAPVKESIKGLKDAPYFQPVDI
                     DLVSLGDETVLIEGHAVSVIRHQYDEYVQMVEARFLLKNPFDSSTLQERSKIEDALKS
                     KYIPVEHRQSGMFEEYTILLVDEVKQAPDKWIDKNALDISRFIRSQRDTFDKNEIAEI
                     LMSRTRYSASELTLVDWEGAVIVAPNGDYDSDVVLLKIGNYQLLRYRMLDHSIENLLD
                     KINESFFENKRRPRATRGLIRQIAEHKLEVMLDFERTEQTLLLIGDWYTAKLYEAIQS
                     EFYLKDWKESLRAKLDNLESIMQTIKDNLSVSWENLMDRIQLLLWMVMLIGYLYLYLL
                     DAKWITIPSN"
BASE COUNT         1454 a         1292 c         1148 g         1323 t
ORIGIN      
        1 aaagcgaccg ggtaactcca tgataccgtc cacttcaaac atggaagtcg tcaggcgcac
       61 ctgatattcc actacattca tatatccccc acgcacaatc ccttgcggac ccaggtcttc
      121 acgccgaggt aaacaaacgg catgaatgcg gctcttcatc gtgcgtacca attcgaaatg
      181 atccactaac ttggtaggca tgtgtaaacg tgccatacgc gcatcatgaa tttccagcgt
      241 cgaatgattg atatcgttta agaaacccat ggcgccatga gtggtcactg caaccttgcc
      301 tgccacacga tacccagaag ttaggatatc tgccagcaca aagcgatatg tacgaggaga
      361 agtgctatca agtgacatgg agaaaccttg agttaagatt caggggtcgg ggagcggaac
      421 cctgtttgag gtcgagggcg aggcggggtg ttgcgatgcg aaggcgtagg cttgccggca
      481 ttcgggtcca agatcatgtt caaccattgg atcgcgcgtt catcatactc gcgccgcccg
      541 cacacatcac acacccaagc cgggaaatgc ggtacggtga tcaactcatg cccgagccag
      601 gtgaagtaag tcacatggcg gggatgcagc aaacccgcat ggcattcgtt acaaggagtt
      661 aatgcattca attcgttcat ggcaaaccct attgtgaaac agccatctcc ggatgatcta
      721 acactttcca aaatttcggg ggccaataga tcaaaacagc cttacccaca acattgccct
      781 gaggcagcaa gccccagtct tttgagtcat tcgagttatt acgattatca cccaacacga
      841 acaaggttcc atccggcacg atccaatcgc cagaatagag cggcgcttga gcaatatagt
      901 tctcgttcaa catctgctcg ttcacatata catggctatc gcgcaccatc acatgatcac
      961 cgggcacccc aataatacgc ttcaccaatt cttcatccgg gttcattggg aagtcgaaca
     1021 caatgatatc gccgcgttga aaatcaccga acttatagct caacttgctc accaacacga
     1081 actcaccatc ttgcaacgtg ggttgcatac taaagccatc cacccgcaca cgtgccgaaa
     1141 cagaattgat gcccacaaat aaaatcactg ccagcaccaa ggtctcaatc acatccagaa
     1201 cgaagcgctt ccaattcgtt gtttcttccg ctggtttcga ttcctctacc aacggttctt
     1261 gttccaaagt atccaaaatg cctccaaact gcccggaaat tatactgcca tttccagaga
     1321 gcctccccat gcttaactct tacttattgg ggatgaaacc ccgcttcatg cgagatgtaa
     1381 agtgggcagc tgccattcgg cttttaccac actctagctt tgatccgcca atcgtagcac
     1441 caccccagag caaaacttgc acctgcaatg gagtcttaac cctcgcagaa acgttgcttt
     1501 gccttatgtc caaaccccgg cgtctcgccc cacttgcgga caaaatctgc ctgttcctct
     1561 gtttcgggcg agcgcaccaa aaagtaattt tgcgggcggg tcttatacaa agcatttacg
     1621 cgctctaagg cttgcgaggg agtcaaccca tgccgcacca tataacatcc caccacggtg
     1681 ccggtccgcc ctaccccacc ccaacagtgg acatacaccg ggcttccgct ttcaatcgca
     1741 gcatcgatgg tatcgagaat caaggtcatc gtctgcaccg aagggatgcc atgatcgcga
     1801 atggcgaagc ggtgataaac cgccatatgt tcatagatac gcgcttcctc tttaaggatc
     1861 ggttcgtatg gaaccaactc atgcggttgg gtcagatcaa taaatgtgcg aataccgctt
     1921 tcaaggaaag catccattcg gcggcgggca tcttcagggt tgtaactgcc cgggtattcg
     1981 cccgctagaa aatgaccttc ttccacccaa taggattcga tgatgggaag atgtttcata
     2041 cgctattgtg ggaaattcac acacacgtta ttgagaattt ttgtgcgcag ttccactagt
     2101 tcagggtatt ggtagaagaa caaaatcttt tgttctgcaa ttctgtctcc atctggcttg
     2161 tctgcgaaaa caaagaaacc aaaggactcc gcaatatcct cagcggggtt ggtcgcagag
     2221 taatctgtca ggaactggtc ttcgtatttg ctgtagaaat catcaagttt ttcgtaataa
     2281 agatcttcgt cttcttcgag gtttatctga ttccactcat cgtaaatatt ggtccagaat
     2341 tggtcataaa aagcgttcat atacgaatcg gggttcgagc aaccttcccc ggggaagaat
     2401 gtggcacaag ccgccacctc ctgcaaatac aggtcgttat catctgggtt attgaacaca
     2461 gcaaggctgg gcggaacctg gtctggacct aaagtgagta aatgggcata ttcatgcaca
     2521 agagtaaagg ttaggtagta ataatcgctg gtgtcagcaa catccacttc taaagaccaa
     2581 cggttcgggt catcgtaggt ctgcgcgacg gctgccaaga cattatccga gccatcggtc
     2641 atgatggaaa attctgccaa ggtagggcga gcctccagcg gaatcactgt agaaaaatat
     2701 tcccacaatt gttggtgcaa tgctgtgtca ttttgctcat ccacaagatc cgcaggcaca
     2761 tcctgctcaa aatacgggtc gcggatttca tttccgtcca ctgtgtatgt gaccaaatat
     2821 atcgcctcat ccccatcatc aacagatgaa tttaaaacat ccaaaggaag ctgattggaa
     2881 aggtctatgg ttgcgttctg gtccacgatc ttgttggtga tcaatggaca ggaaagcgct
     2941 tcaggcgtag ccgtagccgg tatcgccatc ccaggcgcga tcccagccga ggcggatttt
     3001 tgtccaaata atgtaacaca agctaaagaa gaaagaatca gcgcagcgag cgcaaagaga
     3061 aatttcagac ggggcataaa aatatccttg gaattttttt ccaaggatac cataaggaat
     3121 ttttatcgaa ttaacagtct cattacgata ttttctacac aaaatcctca ttatttcatg
     3181 gagcaagcgc cgctcaaaat atcttcacgc aactgcacca gctctgggta ttcataaaag
     3241 aaggcgagct tttggtcacg aatggaattg ccaactggtt tggggttgaa aacaaaatag
     3301 gtaaaagact cggcaatatc ttcggcgggg tgcgttacag cataatcatc tacaaagcga
     3361 tctttgtgct taaggtaaaa cgcatacaac ccgttgtaat atggcactag gtcttctgcc
     3421 ccatattgga gcgcgtccac ttgttgccac tcgtctgaaa tatctgccca aaaacgattg
     3481 taaaaagcat tcatgtatga atcaggatgg gtgcaacctg tgcctacaaa ataagtagaa
     3541 caagctgcgg ctttttcttt ttgcaattca gggttgagtg ggtctttata ggtatctgca
     3601 tctggcgtga cctgagatga attcagggtc aacaagtgag catattcgtg aaccatcgta
     3661 tagataaggg cgtctttatc ttcgaggtca gcaacgtcca cttctagcat ccattgtgag
     3721 aggtcatttt ctgtttggtc aacggctgcc agggtgtttg aatatccatc ggtgaagaca
     3781 ttaaactgag ataccatttg acgattctga acaggaatta gggttgtaaa aatactccat
     3841 gcttcctgtt gcaacacggt atcgtgttgc tcatcctgta aatcttttgg aactgtcttt
     3901 aggataggat cggtgatggt gctttcctct acatcataag aggcaagata ataaaatgcc
     3961 ggttcttcaa aattagtcac tccgctgaag ttgtacacat cgcctttgcc actatcaagc
     4021 acgtgatcca tggaaacagc acaagatgca tatgcatcag gtccaagagg caaaatactc
     4081 ttttgcccaa aatacagccc gataaaaaga aataaaacca gcgaaagtaa aacacccata
     4141 aagcttatgt tcttcgacat gccgcctccg cggagatacc attaatttga agggattgtg
     4201 atccatttcg catctaaaag atagagatac aggtaaccga tcagcatcac catccaaagc
     4261 aataactgaa tcctatccat cagattttcc cacgaaaccg ataaattatc tttgatggtc
     4321 tgcatgatgc tttccaaatt atcgagcttg gcacgcagac tttccttcca atctttgaga
     4381 taaaactctg attgaatggc ttcatacaat ttggcagtat accaatctcc gatcaggagc
     4441 aaggtctgct cggtgcgttc aaaatcgagc atgacttcaa gcttgtgctc cgcgatctga
     4501 cggataagtc cgcgcgtggc gcgtggacgg cgtttattct caaagaaaga ttcgttgatt
     4561 ttatccaaca agttctcgat ggaatgatcc aacatacggt accgtaacaa ttgataattg
     4621 ccgatcttta ataagacgac atccgagtca taatccccat tgggcgcaac tatgacagcc
     4681 ccttcccaat ccaccaaggt caactcgctg gcagaataac gcgtccgtga catgagaatc
     4741 tcagcaatct cattcttatc aaaagtatct cgctgagagc ggataaaacg ggaaatatcg
     4801 agggcattct tatcgatcca tttatcgggt gcttgcttca cctcatccac cagaagaatc
     4861 gtatattctt cgaacatacc gctctggcgg tgttctacgg gaatgtattt gctcttaagt
     4921 gcgtcttcga tcttgctgcg ctcttgcaga gttgatgaat cgaatgggtt cttcagtaag
     4981 aaacgtgctt ccaccatctg cacatattca tcatactgat ggcgaatcac agaaacggcg
     5041 tgaccttcaa tcaaaacggt ctcatccccc aggctaacaa ggtctatatc tacaggctga
     5101 aagtagggtg catctttcaa gcccttgatt gactctttta ccggtgcaac tttatgttct
     5161 gtatcaggaa aagctattaa gtaacaaatc tccatgccat aagtgtagca caggagg
//
LOCUS       DAOQJE010000127         5212 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_9270_length_5212_cov_5.624588, whole genome shotgun sequence.
ACCESSION   DAOQJE010000127 DAOQJE010000000
VERSION     DAOQJE010000127.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 5212)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5212)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5212
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9270_length_5212_cov_5.624588"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..298)
                     /locus_tag="PLT08_04785"
     CDS             complement(<1..298)
                     /locus_tag="PLT08_04785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE03809.1"
                     /translation="MKQRFSIQDLLARTPLGNLNLRGKLTAGNMIITLLVIAVMGIYV
                     YFRIQTASQQLITSVDENARNRAEEDLVNTSREQATFLDSFFVNMTNNTSIATAA"
     gene            complement(314..2629)
                     /locus_tag="PLT08_04790"
     CDS             complement(314..2629)
                     /locus_tag="PLT08_04790"
                     /inference="COORDINATES: protein motif:HMM:NF014763.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HNE03810.1"
                     /translation="MDQTAQTPPEETNFPEHLQQTSLGLKLTLIAVGLLLVAFIVYTF
                     ISIRISQGDLLASVEENLKIKNNETISLIQSRLTEAKGIATNLSVAVESGAFTEGDLR
                     VIIQNTVDRNASIHGATVGYEPNQFKSNIYYWSPYYYRMPDNTIQYLQLGDYDYFNQE
                     WYSVTKKNSAPTLLPPYRNKSHGETWISTWSIPIYNNQGDFKGVAAVEIDLGKIQQTF
                     TSLKFGRTGYAFMIDSSGTILGIGDEGGNFEPMIDSMYVIANTNPASNWITLIDKMTN
                     GEAGYVEVNDRNGDPMFVSYAPVGMDTDWSLALVYPREEVIQQTRRLQASLTAYSFGL
                     ALIFGLVIFFFTRTITNPLEQLTKVAEEISNGNLNISAPVESDDEVGTLAETINRMTA
                     QLQDTYNNLERRITERTVDLEIARRQSEIRTTQLLSIGEISKIINSEQELDILLPLIA
                     RLVSERFNFYHVGIFLIDDARQYAVLQAANSSGGQNMLKRGHKLKVGESGIVGYVAKT
                     GVPRIALDVGQDAVFFNNPDLPNTRSEVALPLKVRENIIGVLDVQSERPGAFSEDDTN
                     IFNILADQVAITLENTRLFEQTQQALDEVRSAYQRNLQEGWKVFGQEEGIIGYQQSIT
                     GGKKLAKPVHLDEIQQAMHRGQVQEFHPDGKTGEPTLVVPIKLRDQIIGVMHIKAPSK
                     ERLWTQDETNLVEAVSERLSLALENARLIQESQRQVIKEQTITEITSRIGSSINLDNV
                     LLTAVEELGRNIPGSEVTIKLKNENKNDGSE"
     gene            complement(2654..4378)
                     /locus_tag="PLT08_04795"
     CDS             complement(2654..4378)
                     /locus_tag="PLT08_04795"
                     /inference="COORDINATES: protein motif:HMM:NF024883.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HNE03811.1"
                     /translation="MLEKLLNLIRSEEDQDPSFIRLTRNILIFVMVVTFAVIPLVTGI
                     AGSPQNRLAFTALSILLVLEAASFYFITRGNVLPAKVVVPLGLVIAITTIANNTNGVR
                     DTSFFALPMILIVSATLLGKRSLTLIVPVAVVATAIIAITDISRGRRAGLVGVDDAII
                     IPLLMAASGVITHLLISRLNESTVRAQKSEQLQKIENQELLVLRATLEERVRERTAEL
                     EIANKVNEKRALQFSAIAKVMNAISSIQTLDELLPLVAKVISEEFNFYHVGIFLLDSR
                     KELAVLRASNSEGGQRMLARNHSLSVGQTGIVGYVTATGQPRIALDVGADSAYFDNPD
                     LPNTRSEIALPLRYAKQIIGALDVQSLEANAFQADDIAILTTLADQVTVAINNALTIA
                     EAKKTLAEAQAAIGQVAEEAWQVLRPTRLGLGFAYSESGVKPLQNPFDNVQAHEAFQK
                     GQTMFTTSPDHKTVLAVPIRLRGSVIGVMQLSNRSNYEKLTQDDAEIAEAVAERLSLA
                     IETATLLQSTQHRADIERITANITSKVSSSSRFETILQTAAQELSRALGGSDVLVQIE
                     PAALKMYT"
     gene            complement(4396..>5212)
                     /locus_tag="PLT08_04800"
     CDS             complement(4396..>5212)
                     /locus_tag="PLT08_04800"
                     /inference="COORDINATES: protein motif:HMM:NF024583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HNE03812.1"
                     /translation="SANSEGGKVMLARGHRLGVGEQGIVGYVTKFGLPRIAVDVGQDA
                     TYFNNPDLPETHSEVALPLRSGSEIIGALDVQSKLTNAFSDEDVNILLTLADQVSIAI
                     QNARSFQQSREALERAERAAAQLTEQQWSQIRKGQKVAGFHFDGIQTQELKSQQKSNQ
                     NNLAIPIILRGVQIGSIKLSTPDADRKWDNNEIAMAQATAERTALAIETARLLEEAQK
                     RASKERTIGHISSTIGSLVNIENIVQTVIQELGDTLPGTDVAIQFTNRQSEQQ"
BASE COUNT         1332 a         1126 c         1170 g         1584 t
ORIGIN      
        1 ttgcagccgt agcaatggat gtgttatttg tcatgttaac gaagaagcta tcaaggaagg
       61 tcgcttgctc gcggctggta ttgactaaat cttcctctgc acggttgcgc gcattttcat
      121 ccacactggt gattaattgc tgactggctg tctggatacg aaaatataca tatataccca
      181 taaccgcaat gacaaggagg gtaataatca tatttcccgc agttagcttt cctcgtaaat
      241 tgaggttgcc aagcggagtt ctagcaagca agtcctgaat tgaaaatctt tgcttcatgg
      301 cttgtttttt tccttattca ctgccatcat tcttgttttc gtttttcaat ttgatggtta
      361 cctctgagcc gggaatgtta cgtcctaatt cttctaccgc agtgagaagt acattatcta
      421 aattgatgga tgaaccaata cggctcgtaa tttcagtgat tgtttgctct ttaatgacct
      481 gacgttgtga ttcttgaatc aggcgtgcat tttcaagcgc aagcgagagg cgttcggaaa
      541 cagcttcgac aagattggtt tcgtcttgtg tccataaacg ttctttcgac ggcgctttga
      601 tgtgcattac accaatgatt tggtcacgaa gcttgatcgg aacaaccaag gtaggttcgc
      661 ctgtctttcc atctggatga aactcttgaa cctgtccacg atgcatggct tgttggattt
      721 catccaagtg aaccggcttg gctaattttt tgcctccggt tatggattgt tgatatccaa
      781 taatgccttc ctcttgacca aaaactttcc aaccttcttg aaggttgcgt tgatacgcgg
      841 atcgcacttc atcaagtgct tgctgagttt gttcaaagag gcgtgtgttt tcaagtgtaa
      901 ttgccacttg gtctgccaga atgttgaaaa tattcgtgtc atcttcgctg aatgcgccag
      961 gtctttcgct ttgcacatct agaacaccaa taatattttc acgcaccttg agaggaagcg
     1021 ccacttcaga acgtgtatta ggaagatctg ggttgttgaa gaagacagca tcttgaccta
     1081 catctagggc gatgcgtgga acgccagttt tagcgacata ccccacgata ccgctctcac
     1141 cgactttgag cttgtgtcca cgcttcagca tgttttgccc gccggaactg ttggctgcct
     1201 gaaggacggc atattgacga gcatcatcga ttaagaagat gccaacatga tagaaattaa
     1261 aacgttcact aaccaaacga gcaataagtg gtagtaggat atctaattcc tgctcagagt
     1321 taatgatttt tgagatttcg ccaatggaaa gaagttgtgt cgtgcgaatt tcactttggc
     1381 ggcgtgcaat ctctaagtct actgtacgtt ctgtgatacg cctttcaaga ttattgtagg
     1441 tgtcctgcaa ctgagccgtc atacggttga tggtctcagc taatgtgcct acttcatcat
     1501 cactttcaac aggggcggaa atatttaaat ttccatttga tatttcttca gcgacctttg
     1561 tgagttgttc aagcgggttt gtaatcgttc gggtaaagaa gaagataact aacccaaaga
     1621 taagcgccaa cccaaaactg taggcagtga gcgatgcttg taatcggcga gtttgttgaa
     1681 ttacctcttc acgtggatac accaatgcca aagaccaatc tgtatccata ccaactggag
     1741 catacgagac aaacatgggg tcgccgttgc gatcattaac ttctacatac cctgcttcgc
     1801 catttgtcat tttgtcgatg agtgtgatcc agtttgaggc ggggtttgta tttgcgatca
     1861 catacatgga atcgatcatc ggctcaaaat taccgccttc atcgccaatg ccgagtatgg
     1921 ttccgcttga gtcgatcatg aaggcatagc ctgttcgacc aaactttaga ctggtaaagg
     1981 tttgttgaat cttgcccaag tcaatttcaa cggcagcaac acctttgaaa tcgccttgat
     2041 tgttgtaaat agggatgctc catgtagaaa tccaagtttc tccatggctc ttgtttctgt
     2101 agggggggag taaggtaggt gccgaatttt tcttagtgac cgagtaccac tcttggttga
     2161 aataatcata gtcgcctaat tgaagatatt gaatggtgtt atctggcatt ctgtaataat
     2221 aaggcgacca ataatagata ttggacttaa attgattcgg ttcataccca acggttgcac
     2281 catgaataga agcattgcga tcaactgtgt tttgaataat tacccgcaga tcaccttctg
     2341 taaaagcacc cgactctact gcaacagata aatttgttgc aattcctttt gcttctgtta
     2401 accggctttg aataagagag atggtctcat tgttcttgat ttttaaattt tcttctacgc
     2461 ttgctaaaag atcgccttga ctaatgcgaa tactaataaa ggtgtacaca ataaatgcaa
     2521 ccagaagtaa gccaactgca attaaggtta gctttaaccc gaggcttgtc tgttgcagat
     2581 gctcgggaaa atttgtttct tctggaggtg tttgagcggt ttgatccata aaaattctct
     2641 ttgtgtaaga cgattaggta tacatcttca gcgcggctgg ttcgatctga acgagcacat
     2701 ctgagccgcc caaggcacgg cttaattctt gagctgctgt ttgaaggatg gtttcaaatc
     2761 tagatgaaga actgacttta ctagtgatgt tggctgtgat tcgctcaata tcggcacgat
     2821 gttgtgtaga ttgaagcaag gttgctgttt caatcgcaag agagaggcgt tctgctaccg
     2881 cttctgcgat ttctgcatca tcctgggtca atttttcata gttactgcgg ttgctcaatt
     2941 gcattacgcc aattacgctt ccacgcaagc ggataggaac agccagaacc gttttgtgat
     3001 ctgggctggt ggtgaacatg gtttgtccct tttgaaaggc ttcgtgtgcc tgaacattat
     3061 caaacgggtt ttgaagtggc tttacaccag attcagaata tgcaaagcct agcccaaggc
     3121 gagtggggcg taaaacctgc caggcttctt ctgcaacttg accaattgcc gcttgagctt
     3181 ctgccaatgt ctttttggct tctgcaatgg tcaaagcatt attaatagca actgttactt
     3241 gatctgcaag tgttgttaat atagcaatgt catctgcttg aaatgcattg gcttccaaac
     3301 tttgtacatc taatgcgcca ataatttgct tggcgtagcg cagaggcaaa gcgatctcag
     3361 agcgggtgtt tgggaggtct ggattgtcaa agtaagcaga atccgctccc acgtccagag
     3421 cgatgcgagg ttgtcctgtt gctgttacgt accccacaat acctgtttgt ccaacagaaa
     3481 gagagtggtt acgggcaagc atcctttgtc caccttcact gtttgaagcg cgtagaacgg
     3541 ctagctcttt cctgctatca agcagaaaga taccaacatg gtaaaaattg aattcttcgc
     3601 tgatcacttt tgcaacaaga ggcagcaatt catcaagagt ttgaatggac gaaattgcgt
     3661 tcataacttt tgcaatggca ctaaactgta gcgccctttt ttcattgacc ttgtttgcta
     3721 tttcaagttc tgccgttctt tctcgtaccc tttcttcaag ggtggcgcgc aagacaagca
     3781 actcttgatt ttctatcttt tgtagctgtt cacttttttg agcccgtacc gtactttcat
     3841 taaggcgaga tatcaacaga tgtgtgatta cgccgctggc tgccattagc aaaggaatga
     3901 tgattgcgtc gtcaacacct accaagcctg ctcgacgacc acggcttata tccgtaatgg
     3961 caattattgc tgtcgcaact actgctactg gcacaataag agtcagcgaa cgtttgccta
     4021 ataaagtagc agagacgatc aagatcatag gaagagcgaa gaaagacgta tcacgtacac
     4081 cgtttgtgtt gtttgcgatg gtggtgatgg cgatgaccaa cccaagtgga actactactt
     4141 tggcaggtaa tacattccct ctagtaatga agtagaacga agcagcttcc aataccaata
     4201 atattgacag ggcggtaaat gcaaggcggt tttgaggtga accagcaatc cccgtcacta
     4261 gtggtatcac tgcaaaagtt acgaccataa caaagatcaa aatattccgc gtcaaacgaa
     4321 taaatgacgg gtcttgatct tcttcgcttc gaattaggtt tagtaatttt tctagcatat
     4381 ttggctcctg ggcggctatt gctgctcgga ttgtctgttt gtaaattgaa tggcaacatc
     4441 agtgccgggc aatgtatcac ctaattcctg aatgacagtt tgtacaatgt tttcgatatt
     4501 gaccaaacta ccgatcgtgg atgagatgtg cccaatcgtg cgttcttttg aagcacgttt
     4561 ttgtgcttct tccagaaggc gtgcagtttc aatagcaaga gctgttcttt ctgcggtcgc
     4621 ctgtgccatg gcaatttcat tattgtccca ttttctgtca gcatcaggtg tgcttagctt
     4681 aatagagcca atttgcacgc cgcgcaatat gatcggaatg gcaaggttgt tttgattact
     4741 tttttgttgt gacttaagtt cctgagtctg aatgccatca aagtggaacc cggcgacttt
     4801 ttgcccttta cggatctgac tccattgttg ttcagttaac tgcgctgccg ctcgctctgc
     4861 tctttcaagc gcttcacgac tttgttggaa tgatcttgca ttttgaatcg caatactcac
     4921 ctggtctgca agtgtcaaaa ggatattaac atcttcgtca ctaaacgcat ttgttaattt
     4981 actttgcaca tccaatgcac cgatgatctc ggatccgctt cggaggggca gggcaacttc
     5041 agaatgagtc tctggaagat ctgggttatt gaagtaggtg gcatcttgcc caacatcaac
     5101 agcaatacgt ggtaagccaa atttagtgac ataccctaca ataccttgtt caccaactcc
     5161 tagacggtga cctctggcta acatgacctt accaccttca ctatttgctg ac
//
LOCUS       DAOQJE010000128         5199 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_9314_length_5199_cov_6.448872, whole genome shotgun sequence.
ACCESSION   DAOQJE010000128 DAOQJE010000000
VERSION     DAOQJE010000128.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 5199)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5199)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5199
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9314_length_5199_cov_6.448872"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1406
                     /locus_tag="PLT08_04805"
     CDS             <1..1406
                     /locus_tag="PLT08_04805"
                     /inference="COORDINATES: protein motif:HMM:NF012264.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hydrogenase iron-sulfur subunit"
                     /protein_id="HNE03813.1"
                     /translation="WLMPRAKALKPVKVDLAHCDGCTLCERDCPYLAIKMVPRTDGAR
                     PKFQAEIDPNLCVSCGVCIGSCPDNALTFGDIPLDPMWKNTLEKVTEKKNVKVVFTCE
                     RHAMHGAGEHFNDPNVHIVPLTCIAMANPNLAAQALEAGASDVQFIGCPPEDCANREG
                     NVWLNDRVNGERLPKLKPNFIPLVHTAWAAPTNFGSAIKSQVKSEANTFSFIPNSSHI
                     RFVLPLLGVMAVVTAAQLWLSNRPVTFFTDDFATLAIQMTHHSGYAIHDITPPVDIEP
                     DLTQPIRITLEVDHQPFFDETYTATDNHINQGARIFEQIQLPVGRHHITIKMYDRADA
                     SYEQILFDDTVNLEPRQSLTINFRDVHLPDPKMGESIYYETASGVNAGCRICHSLTKG
                     EKIIGPSFYGIADRAGERVPGLTAEEYLHQSIVEPNAFVVPGYPSGQMIQNFGQILTE
                     EQIQDLIAFLMTQKEDK"
     gene            1459..1890
                     /locus_tag="PLT08_04810"
     CDS             1459..1890
                     /locus_tag="PLT08_04810"
                     /inference="COORDINATES: protein motif:HMM:NF025012.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HNE03814.1"
                     /translation="MKRTSVLLLLLVLALALTACGRKPPAPLGADAPADNVPEIIGEY
                     GLNGEDATHEQYGGSLFITAGNQPGEYNLQWLVSGDIQEGIGTLAGNQLTFTWKSVEG
                     TDQSLSGYGTYTITVNGELYGERFVDGVAEPGIESVYPNPK"
     gene            2023..2151
                     /locus_tag="PLT08_04815"
     CDS             2023..2151
                     /locus_tag="PLT08_04815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE03815.1"
                     /translation="MNGVIHLGSFSGISGTLYTTPYDVPATVFVYKTDGEVFRYLS"
     gene            2180..3619
                     /locus_tag="PLT08_04820"
     CDS             2180..3619
                     /locus_tag="PLT08_04820"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HNE03816.1"
                     /translation="MAKVVVIGAGFAGHTAALYLGRRLGKKHEITVISNRDVFGYVPS
                     WVWVGVGHMKPEQTIFKLKPVYDKMNVKFVHAAAKEIHPDEGDQYVLGEEVGSGKAVR
                     LNYDYLVIAPGPLLNFAGTPGLGPENGFTHSICSLPHAMKARDAYLALCERMKKGEKV
                     KIVIGTGHPGATCQGAAFEYIVNVHKDLVKKGLRDNADILWLSNEPAMGDFGVNGVQI
                     KRNGHVMKSEEFLTSVFNDHGIRYQVQTGVTKVEDGKIHWQNYEGEEGTTEFDFSMLI
                     PQFKGQPFKYIDKNGEDITSKLTNPAGFVLVDGKYGLPYPELAQTPDAWPSQYQNPTY
                     KNVFAAGIAFAPPGPISKPFTNKNNLLIAPAPPRTGMVSGVIGRVVALNIADLIEKGE
                     MTHSEPMTEMVAACIASMGDSLWDGNAVSIVMKPVVPDRKKYANEFGRDLFTSHLEMG
                     VSGAWMKFMLHHTFIWKFKARPGWTIIPE"
     gene            3630..3755
                     /locus_tag="PLT08_04825"
     CDS             3630..3755
                     /locus_tag="PLT08_04825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE03817.1"
                     /translation="MSSQEFSKAEKFWMNFKLYQLWRFVVLNVKILMTVYQSKRS"
     gene            4001..>5199
                     /locus_tag="PLT08_04830"
     CDS             4001..>5199
                     /locus_tag="PLT08_04830"
                     /inference="COORDINATES: protein motif:HMM:NF013791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0070069 - cytochrome complex [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0019646 - aerobic electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome ubiquinol oxidase subunit I"
                     /protein_id="HNE03818.1"
                     /translation="MDPITLSRWQFGLTTVYHFLFVPLTLGLSWFVAYFQTRYYQTKD
                     ETYRKMTKFWGKLFLINFAIGVVTGIVQEFQFGMNWSEYSRFVGDIFGAPLAVEALMA
                     FFLESTFLGVWIFGEGKVSEKAHLTSIWLAAIGSNLSALWILLANGWMQHPVGFVINE
                     VTGRAELVDFFALISNPKGWLFFWHTISAGLTTASFLILGVSAYHLIRKQNVDFFKRS
                     FQTATIVGLVASTLVFFGGHTNGQYMFETQPMKFSSIEAHWETSQPADFSILTIGDLS
                     GKREVWSFRTSQLGIPGVLSFLACNNFDCEVRGVNDIQAEYEATYGPGDYIPLMVVTY
                     WTFRFMMSIGFLMMLLSFLFLLALRGNFENAKWMKWVPYVLVLPYIANMSGWILTEMG
                     RQPWIVQG"
BASE COUNT         1279 a         1464 c         1211 g         1245 t
ORIGIN      
        1 cctggcttat gccgcgtgcg aaagcgctca aacccgtcaa agtggatctt gcccattgcg
       61 atggctgtac gctttgcgaa cgagactgcc cctaccttgc catcaaaatg gtgccgcgca
      121 ctgatggtgc ccgcccgaaa ttccaagccg aaatcgaccc aaacttatgc gtctcgtgtg
      181 gggtgtgtat tggctcctgc cccgataatg ccctaacatt cggcgatatt ccgctcgacc
      241 cgatgtggaa gaacacactt gaaaaagtga ccgaaaagaa aaatgtaaaa gttgtcttca
      301 cttgtgaacg tcatgccatg cacggcgcgg gcgagcactt caacgacccg aacgttcaca
      361 tcgtcccgct cacttgtatc gcgatggcga atcctaatct cgccgcacag gctctcgaag
      421 ccggtgccag tgatgtgcaa ttcatcggct gcccgcctga agattgcgcc aatcgtgaag
      481 gcaacgtctg gcttaatgac cgtgtcaacg gggagcgctt gccgaagctc aaaccgaact
      541 tcattccgtt ggtgcatacg gcatgggcgg cgcccacaaa ctttgggagc gcaatcaagt
      601 ctcaggttaa gtctgaagcg aacaccttct cattcattcc gaattccagt cacatccgtt
      661 tcgtgctgcc cttgctcggt gtgatggctg ttgtcacagc agcgcagtta tggctaagca
      721 accgcccagt gactttcttt acagatgact ttgccacgct ggcgatccaa atgacccatc
      781 acagcggcta tgccattcat gacatcacgc ctcctgtgga cattgagcct gatctgaccc
      841 aaccgattcg cattacgctg gaagtggatc accaaccctt cttcgacgaa acctataccg
      901 cgaccgataa tcacatcaac caaggcgcac gcatcttcga gcagattcaa ctgcccgttg
      961 gcagacatca catcaccatc aagatgtatg accgtgccga tgccagctat gagcaaatcc
     1021 tctttgacga taccgtcaac ctggagccgc gccaatcgct gaccatcaac ttcagagacg
     1081 ttcacctgcc agacccgaag atgggcgaat cgatttatta cgaaaccgct tctggggtca
     1141 atgctggttg ccgcatttgt cactcactga ccaaaggcga aaagatcatc ggtccgtctt
     1201 tctacggtat cgcagaccgt gccggtgaac gtgtgcctgg cttaaccgcc gaagagtacc
     1261 tgcatcaatc cattgtggag ccaaatgctt ttgtggtgcc tggctacccc agtggacaga
     1321 tgattcaaaa cttcgggcag atcctcaccg aagaacagat tcaagacctg atcgcattct
     1381 tgatgaccca aaaagaggat aaataaaatc taattgaccg tcacttttta gggtgatcgt
     1441 catctaagtt gaggaactat gaaacgaacc agcgtgctac ttttattact tgtccttgct
     1501 ctcgccctca ccgcctgcgg acgcaagccc cctgcccctc tcggcgcaga tgcccccgca
     1561 gataatgtgc ccgaaatcat tggtgaatac gggttgaatg gtgaagatgc cacccacgaa
     1621 caatatggcg gctcgttatt tatcacagca ggcaaccagc cgggtgaata caaccttcaa
     1681 tggctggtct ctggcgatat tcaagaaggg attggcacgc ttgcaggcaa tcaactaacc
     1741 ttcacctgga agagcgtgga aggcacagac caatctctga gcggttacgg tacttacacc
     1801 atcaccgtga acggagaact ctacggtgag cgttttgtag acggtgtcgc tgaacccggc
     1861 atcgagtcgg tatacccaaa tccaaaataa tcagttttca tcactctgct cgtagcaagt
     1921 catctataaa acaactttaa taagaactgt cagcccaata tcgagagacg tgcatcgctt
     1981 tatcgttcga tgaccgtcgt gaagtccaca tcatgtattc agatgaatgg agtaattcac
     2041 cttggctcgt tttcaggcat ttcaggcaca ctttacacaa caccctatga tgtgcctgca
     2101 acagttttcg tctacaagac agatggcgaa gtctttcgct atctgtcttg attcttaatt
     2161 cccaaaggag agaaagaata tggcaaaagt tgtagtgatt ggagcagggt tcgctggtca
     2221 tactgcggca ctgtatctag ggcgtcgtct cggtaaaaaa cacgagatca ccgtgattag
     2281 taatcgggat gtgtttggct acgtcccttc atgggtttgg gtcggcgttg ggcatatgaa
     2341 acccgagcag accatcttca aactcaaacc cgtgtacgac aaaatgaatg tcaagttcgt
     2401 gcatgccgcc gccaaagaaa tccaccccga tgaaggcgac caatacgtgc tgggggaaga
     2461 agtcggctcg ggcaaagcgg tgcgcctgaa ctatgattac ctcgtcatcg ctcccggacc
     2521 tttactcaac tttgccggca cgcccggtct cggacccgag aacggattca cccactccat
     2581 ttgttctctg ccccacgcca tgaaagcgcg cgatgcttac ttggctttgt gcgaacgcat
     2641 gaagaaaggc gagaaggtta agatcgtcat cggtaccggt caccccggcg cgacgtgtca
     2701 gggtgcggcg tttgagtaca tcgtcaatgt tcacaaagac cttgtcaaaa agggactgcg
     2761 cgacaatgcc gatatcctct ggctctcgaa cgaacctgcc atgggcgact tcggcgtcaa
     2821 cggggttcag atcaaacgca atggtcatgt gatgaagagc gaagaattcc tcacctcggt
     2881 cttcaacgat cacggcattc gctaccaagt tcaaaccggc gtcaccaagg tcgaagatgg
     2941 caagatccac tggcaaaact acgaaggcga agaaggcacc acagaattcg acttctcgat
     3001 gctcatccca cagttcaaag ggcagccctt caaatacatc gacaagaacg gcgaagatat
     3061 cacctccaaa ttgaccaacc ctgccggctt tgtattggtg gatggaaaat acggtcttcc
     3121 ctatcccgag cttgctcaaa ccccagatgc ctggccctcc cagtatcaga atcccaccta
     3181 taagaacgtc tttgctgccg gcattgcctt cgcccctccc gggccgatct ccaaaccgtt
     3241 caccaacaag aacaatctgc tcattgcccc tgccccaccg cgcaccggca tggtcagcgg
     3301 tgtgatcgga cgtgtcgtag cgctcaacat cgcagactta atcgaaaaag gcgaaatgac
     3361 ccacagcgaa cctatgaccg aaatggtcgc cgcctgtatt gccagcatgg gcgactcgct
     3421 ctgggatggc aacgccgtct ccattgtgat gaaacccgtt gtgccagacc gcaaaaaata
     3481 cgccaacgaa ttcggtcgtg acctgttcac ctctcacctc gaaatgggcg tcagcggcgc
     3541 gtggatgaag tttatgctgc accacacctt catctggaag tttaaagccc gccccggctg
     3601 gaccatcatc cctgaatagg agccttgcca tgtcttcaca agaattctca aaagccgaaa
     3661 agttttggat gaacttcaaa ctctatcaac tttggcgttt tgtggtctta aacgtcaaaa
     3721 tcttaatgac ggtttatcag agcaaacgct cttaaacatt ctcccctctt gttaaaagcg
     3781 ccaagccttg gtttggtgct tttaattttg cccaaaacgt gcaacaaata aattacataa
     3841 caaaataata acattacaat agtcaagcca tgtttgtgac attattcacg cccacctcat
     3901 aagtttaatg tttacaataa tggtaatcgt tgaccaaagt agtcaaggtt ttgaccaatt
     3961 tggataaagt tagccgatta tttttttgga gaacacatct atggatccaa tcactctctc
     4021 acggtggcaa ttcggtctca caaccgttta ccacttcctc ttcgtccccc tcaccctcgg
     4081 gctttcctgg tttgtagcgt acttccaaac ccgctactac caaaccaagg atgagaccta
     4141 ccgcaaaatg accaagttct ggggaaaact cttcctcatc aactttgcca ttggcgtagt
     4201 caccggcatt gtgcaggaat ttcagttcgg tatgaactgg tctgaatatt cccgcttcgt
     4261 cggcgatatt ttcggcgctc cgctcgcagt ggaagcgctc atggcgttct tcctcgaatc
     4321 gaccttcctc ggcgtctgga tcttcggtga aggcaaagtt tcggaaaaag cgcatcttac
     4381 ctcgatctgg cttgccgcga tcggctcgaa cctctctgcc ctttggattc tgctcgccaa
     4441 cggctggatg cagcacccgg tcggcttcgt gatcaacgaa gtaaccggtc gcgcggaact
     4501 ggtcgatttc ttcgcactca tcagcaaccc caaaggttgg ttgttcttct ggcacaccat
     4561 ctctgccggt ctcaccaccg ccagcttcct cattctcggt gtgagtgcct atcacctcat
     4621 ccgcaaacag aacgtggatt tcttcaagcg ctccttccag accgccacta tcgtgggtct
     4681 cgttgccagc accttggtct tcttcggcgg tcacacgaac ggacagtaca tgttcgaaac
     4741 ccagccgatg aaattctcct ccattgaagc gcactgggaa acctcacaac ctgccgattt
     4801 ctccatcctc accatcggtg atttgagcgg caagcgtgaa gtctggtcat tccgcacgag
     4861 tcagctcggc atccccggag tcttaagttt cctagcttgt aacaacttcg actgcgaagt
     4921 gcgaggcgtg aacgacatcc aggctgaata tgaggccacc tacggtcccg gcgattacat
     4981 ccccttgatg gtcgtcacct actggacctt ccgcttcatg atgagcatcg gcttcttgat
     5041 gatgttactt tccttcctct tcttgctggc tctacgcggt aactttgaaa atgccaagtg
     5101 gatgaagtgg gttccgtacg tgcttgttct gccttacatc gccaacatga gcggatggat
     5161 cctgaccgaa atgggacgcc aaccgtggat cgttcaagg
//
LOCUS       DAOQJE010000129         5192 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_9331_length_5192_cov_4.825774, whole genome shotgun sequence.
ACCESSION   DAOQJE010000129 DAOQJE010000000
VERSION     DAOQJE010000129.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 5192)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5192)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5192
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9331_length_5192_cov_4.825774"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..778)
                     /locus_tag="PLT08_04835"
     CDS             complement(<1..778)
                     /locus_tag="PLT08_04835"
                     /inference="COORDINATES: protein motif:HMM:NF014477.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HNE03819.1"
                     /translation="METLSFRAMNTSVLLAVDGEPYAAVGLQEARSVIEQFEKRFSRF
                     LPDSELSQLNASHGAWTSVSPELFEMLDLALSYYQDTNGLFDPSILPNLKRLGYDRSM
                     DQLRKQPPSLRRSLPFPRADFAALELDPAQKRVRLPKGMEIDLGGIAKGWIVERTAQL
                     LSQYAEVCAVNAGGDILFVGEPLDGFGWDVYLEDPHNPNQSLVPLHVRSGAVATSSIT
                     KRAWIQGAESRHHLIDPRTGQPAVSGWLSVTVFSASLVQAE"
     gene            complement(782..1036)
                     /locus_tag="PLT08_04840"
     CDS             complement(782..1036)
                     /locus_tag="PLT08_04840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE03820.1"
                     /translation="MKLFKFGLRFWITLGSVFSFLIGWVIFGHSLKPTQPVSQANLTT
                     LPTLEPLPPMGGLQDSRNRQFPQISQPSFSSRPMFMTSGS"
     gene            complement(1045..1428)
                     /locus_tag="PLT08_04845"
     CDS             complement(1045..1428)
                     /locus_tag="PLT08_04845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE03821.1"
                     /translation="MKKQFSAFIVASLITVIVAAGLLLIGGAVYFNQNGTQMQSTSVS
                     SSSAGTSNAPSQEVAQLQSLVQQYQEREKEYQAREQQYQAQLASANQQLQMDQQQFQQ
                     VNMLLVELQQRGVITVSDGRVFINQ"
     gene            1577..2734
                     /locus_tag="PLT08_04850"
     CDS             1577..2734
                     /locus_tag="PLT08_04850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE03822.1"
                     /translation="MDSLQTLKELSSQMQFEADGEPKVHPGAPVCYSPKEDNNAFTHA
                     AQLPNGQKIVLLKTLLSSACERDCFYCPFRAGRDFRRATFKPDEFAGLFSKMTQSGFA
                     QGVFLSSGIAAGGVRTMDKLLATADILRKKHNFKGYLHLKVMPGSEKDQVYRAMQLAD
                     RVSVNLEAPSTERLAKLAPHKIFIEELMRPLRWVEEIRRTVPAYKFWGGKYPSTVTQF
                     VAGGSDESDLELLNTTQWLMKNVRLKRAYFSAFHPIRDTPLENKTAVDPIREHRLYQA
                     SFLLRDYGFDLEDLPFTADENLPLHTDPKLAWAQMNLTHTPVEVNRADRRELMRVPGI
                     GQKGADAILSARRLNKLRDISALKKLGIIAERAAPYVLLDGKRLASQLALF"
     gene            2833..4182
                     /locus_tag="PLT08_04855"
     CDS             2833..4182
                     /locus_tag="PLT08_04855"
                     /inference="COORDINATES: protein motif:HMM:NF013998.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HNE03823.1"
                     /translation="MLLPKRTTQRLRLPTPSYNPHLKSTLRLNAKAFPVIAIIALILQ
                     IITPSRVWVTLWIVLGGVWLLCWWWVKGLSRSLEFKRELRFGWAQVGDKLEERFTINN
                     PFILPVIWIAVDDHSTLPDHYASLVISVDGRSSFQWKLNSQCTHRGVFTLGSTTLETG
                     DPFGIYTLKFEDPSSSTVVVMPPVISLPRFNILASGWAGDGRPSPRALEETTNISHTR
                     EMVSGDSLRLVHWKTSARKNKLHVREFDGTQAGNWWILLDLYKDSQVGTGWDSTEEHA
                     VILAASLIVQGLNEEHPVGLAVNGHEPTWHIPRRNEYQSHALLKALAIAKPSEHPLRE
                     FIQRNSKSLGQRSSLLIITASHDPTWVEDLIPLMRRGILPTIFLFDVNTFGAQADSRS
                     LSSTLAELGVPCHIIPKDLVGSQQIRRGHEGEWKWRVTGTGKAVAVQQPIADWRALE"
     gene            4179..>5192
                     /locus_tag="PLT08_04860"
     CDS             4179..>5192
                     /locus_tag="PLT08_04860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE03824.1"
                     /translation="MMQRLTRQLVIADVLACVIVIAALKIILTGISASVPNTVNNQYF
                     FVIGLVATSIGFGLSKAQRNSIQASAGIAALGILLVWIIGARLPQPLFDLVSAIFNTL
                     KQVILSIQHDTAVETTAIQSAWAVIASSSVTLWSRLQTWLAGFTSKTVNDALMRSLAW
                     ILLYWVISAWLGWYTNKRNALIAFLPALFLLTALTAYSEKKIDSVLALLASLLLLMGI
                     WHYKSHMALWMKRRLDFSESLPLDVGQAIIAITVAVVLLAALTPSLSWDDLVNLIRRN
                     NTDSVAKMLGVRPPATSNGSGTTQPPSMPREHLLTGGFANSEKVVMTIRTGELPPLNV
                     ETLP"
BASE COUNT         1240 a         1412 c         1229 g         1311 t
ORIGIN      
        1 cttcggcttg cacaaggctg gcgctgaaca ccgtcacgct cagccaacct gaaaccgccg
       61 gttgtcctgt gcgagggtca atgagatgat gtcggctttc cgcgccctga atccacgccc
      121 gtttggtgat ggatgaagtt gccaccgctc ccgagcggac gtgcaacggc accaaacttt
      181 ggttcgggtt gtgtgggtct tccagatata catcccagcc gaaaccgtct aacggctcac
      241 caacgaacag gatatctccg cccgcgttta ccgcacacac ctcagcatat tgactcaaga
      301 gctgcgcggt tctttctacg atccagcctt tggcaatgcc gcccaggtcg atctccatgc
      361 ctttgggcaa ccgaacacgc ttctgggcag ggtccagttc aagcgcggcg aagtctgctc
      421 gtgggaatgg cagagaacgc ctaaggctag ggggctgctt gcggagctgg tccatgctgc
      481 ggtcatagcc gaggcgtttt aaattgggca gaatcgacgg gtcaaagagt ccattcgtgt
      541 cctggtaata agaaagcgcc aggtccagca tctcgaatag ctcaggcgaa acactcgtcc
      601 aagccccatg tgaggcgttt agttgtgaaa gctcgctatc tggcaagaag cggctaaaac
      661 gcttttcaaa ctgttcaatg acagaacgcg cctcttgcaa gccgacggcg gcatacggct
      721 ctccgtccac tgccaacagc acggaggtgt tcattgcccg aaacgaaagg gtttccattt
      781 gttacgagcc gctcgtcatg aacatggggc gcgaagagaa agaaggctgc gaaatctgag
      841 ggaattgccg gttgcgcgaa tcttgcagtc cgcccatggg gggtaaaggc tccagcgtgg
      901 gtaaggtagt taagtttgcc tgagaaaccg gctgcgtagg cttcagcgaa tgtccaaaga
      961 tcacccaccc gatcaaaaag ctgaagacac tgccgagggt gatccaaaaa cgtaaaccaa
     1021 atttaaagag tttcatagtt gcctctattg attgataaaa accctgccgt ctgaaacggt
     1081 gattacgccg cgttgttgca actccaccaa aagcatgttg acctgttgaa actgttgctg
     1141 gtccatttgc agttgctggt tggcagacgc caattgcgcc tgatactgct gctcgcgagc
     1201 ctgatattcc ttttcacgtt cctgatactg ctgtacaagg ctttgaagct gtgccacctc
     1261 ttgggagggg gcgttacttg tacctgcact agacgaggaa acgctggtac tctgcatttg
     1321 tgtgccattt tggttgaagt acaccgcccc accgatcaac aacagccccg ccgccacgat
     1381 aacggtgatg aggcttgcca caataaatgc cgaaaattgc tttttcatct ggtttctcca
     1441 gtagttctca atttggtagt tgagatggta tctgaaccct ctggtgaatt ggtgaagaga
     1501 ttttgaaggt tatatgaatc taaaatgagt aaaaaaacaa cttgaaaatt tagaacatat
     1561 attctataat gagtacatgg actccctcca gactctcaag gaactatcct ctcaaatgca
     1621 gttcgaagcc gacggcgaac ccaaagttca ccccggcgca ccggtctgtt actcaccgaa
     1681 agaggataac aacgctttca cgcatgccgc ccagctccct aacgggcaga agatcgtctt
     1741 gctcaagact ctgctttctt cggcttgtga gcgcgattgt ttctactgtc cattcagggc
     1801 agggcgtgat ttccgccgag ccaccttcaa gcccgatgaa ttcgccggtt tgttcagcaa
     1861 aatgacccag agtggcttcg ctcagggtgt tttcctaagc tccggcattg cagccggagg
     1921 cgtccgcacc atggataaac tgctcgccac tgcggacatc ctaagaaaaa agcataactt
     1981 caaaggctac ctgcatctca aggtcatgcc gggttctgag aaagaccaag tctatcgcgc
     2041 catgcagctc gccgaccgcg tctcggtcaa cctcgaagcg ccaagcaccg aacgcctcgc
     2101 caagctcgca ccccataaaa ttttcatcga agaactcatg cgcccactca ggtgggttga
     2161 agagattcgc cgaactgtcc ctgcatataa attttggggc ggcaagtatc cctctacagt
     2221 gacgcaattt gtagcaggcg ggtctgatga aagcgacctt gaactgctca acaccactca
     2281 atggctgatg aagaacgtgc gcctcaaacg cgcttatttc tctgccttcc atcccattcg
     2341 tgatacaccc ctcgaaaaca aaaccgccgt cgaccccatc cgcgagcatc gcctttatca
     2401 agcctcattc ttattgcgcg actatggctt cgacctcgaa gacttgccct tcacagcgga
     2461 tgaaaacctt ccgcttcaca ccgacccgaa actggcgtgg gcgcaaatga acttgactca
     2521 cactccagtc gaagtcaacc gcgccgaccg ccgcgaattg atgcgtgtac cgggcattgg
     2581 tcaaaaaggt gccgacgcca tcctctccgc tcgcaggctc aacaaactgc gtgatatctc
     2641 tgccctgaaa aagcttggca tcatcgctga gcgtgccgcc ccttatgttt tgctagatgg
     2701 taaacgcttg gcaagtcagc tcgctttgtt ttaaagttca acacctccta ccccgtcgta
     2761 taatcgatat ataaagtttg ttccacggtt gccttactgc aaccgcatct ttattttgcc
     2821 gtctgggtcg ctatgttact tcctaaaaga acgacgcaac gcctacgcct tcccactcca
     2881 tcgtataacc cgcatttgaa atctactttg cgattgaatg caaaggcgtt ccctgtcatt
     2941 gccatcatcg cactcatact tcagatcatt acaccatcac gagtgtgggt cactttatgg
     3001 attgtcttgg gcggcgtttg gcttctttgc tggtggtggg tcaaaggtct ttctcgctca
     3061 cttgaattca aacgtgaact gcgctttggc tgggctcaag tcggcgataa actcgaagaa
     3121 cgcttcacga tcaacaatcc attcattttg ccggtcattt ggattgctgt agatgaccac
     3181 tccactctgc ccgatcacta tgcatccctc gtcatcagtg tcgacgggcg atcttctttt
     3241 caatggaagc tcaattcgca atgcactcat cgcggagttt tcacactggg tagcacaaca
     3301 cttgaaaccg gcgacccttt tggaatctat accctcaagt tcgaagatcc ttcctcatct
     3361 actgttgtag tcatgccacc ggtcatttct ctgccacgct tcaacatcct tgccagcggt
     3421 tgggcaggtg atggaagacc cagtccacgt gcattggaag aaacaaccaa tatctctcac
     3481 actcgtgaaa tggtgtcagg agattcattg cgcctcgtgc attggaagac cagtgcccgc
     3541 aagaacaaac tccacgtccg cgaatttgat ggcacccaag ctggcaactg gtggattctt
     3601 ctcgacctgt acaaagactc gcaagtcggc acaggctggg actctaccga agaacacgcc
     3661 gtcattctgg cagcgtcgct cattgttcaa ggtctaaatg aggagcatcc cgttgggtta
     3721 gccgtcaatg ggcatgaacc cacctggcat ataccgcgcc gaaatgaata tcaatctcat
     3781 gctttgctca aagcgttggc aatcgccaaa ccttcagagc acccactcag agaatttatt
     3841 caacgcaaca gcaaatcgct tggtcagcgc agcagtctgt taatcatcac cgcctcgcat
     3901 gatcctacat gggtcgagga tcttattccg cttatgcgcc gtggaatatt acccaccatc
     3961 tttcttttcg atgtgaacac cttcggcgca caggctgatt cccgctcgtt atcgtccacg
     4021 ttggctgagc tcggcgtgcc atgtcatatc atccctaaag acttggtagg gtcacagcaa
     4081 attcggcgtg gtcatgaagg cgaatggaaa tggcgagtca ctggcactgg caaagccgtt
     4141 gccgtgcaac agcccattgc cgattggaga gcgctagaat gatgcagcgc ttaacacgtc
     4201 agctcgtcat agcagatgtt ttggcatgtg tgattgtcat cgcagcgctc aagatcatac
     4261 taacgggtat ttcagcctct gtgcccaaca cagtaaacaa tcaatatttt tttgtaatcg
     4321 gtctcgtcgc tacgagcatc ggattcggtt tgagtaaagc tcaacgtaac agcattcaag
     4381 cttcggcggg gattgcggcg ttgggcatcc tccttgtttg gatcattggc gcgcgtctcc
     4441 ctcaaccttt atttgacctt gtttctgcga tattcaacac actcaagcaa gtcatcttat
     4501 cgattcaaca cgacactgca gtagaaacga ctgctattca atccgcttgg gcagttattg
     4561 cttcttcttc tgtcacatta tggtctcggt tgcaaacttg gcttgcaggc ttcacctcaa
     4621 aaacggtgaa cgacgccctt atgcgcagtt tggcttggat tttgttgtat tgggtcatct
     4681 ctgcttggct aggttggtat acgaataaac gcaatgcctt aatcgctttt ctgcctgctt
     4741 tatttttgct aacggcactc acagcatata gtgagaagaa aatagactct gtattagccc
     4801 tcttggcatc tttgctttta ctcatgggaa tttggcacta caaatctcat atggctttat
     4861 ggatgaaaag gcgcttagat ttttcagaaa gccttccatt ggatgtaggg caagccatca
     4921 ttgcgatcac agttgcagtt gtcttactgg ctgcacttac cccatcgctt tcatgggatg
     4981 accttgttaa tttgatacgc agaaacaata ctgactcggt tgccaaaatg ttaggggtgc
     5041 gtcctcctgc cacatccaat ggttctggca caacacaacc accgtccatg ccacgcgaac
     5101 atcttctcac tggcggcttt gccaactctg aaaaggtcgt catgacgatt cgaacaggtg
     5161 aacttccgcc gttgaatgtc gaaacgttgc ct
//
LOCUS       DAOQJE010000130         5185 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_9348_length_5185_cov_7.364522, whole genome shotgun sequence.
ACCESSION   DAOQJE010000130 DAOQJE010000000
VERSION     DAOQJE010000130.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 5185)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5185)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5185
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9348_length_5185_cov_7.364522"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            115..597
                     /locus_tag="PLT08_04865"
     CDS             115..597
                     /locus_tag="PLT08_04865"
                     /inference="COORDINATES: protein motif:HMM:NF020496.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdeI/OmpD-associated family protein"
                     /protein_id="HNE03825.1"
                     /translation="MIKFRAKIEIIGVNPFVFLPEHALTEIFKQANITKGKIPIKMKI
                     DGHEFTQTLIKWQGAWRLYLNMPMRKAAQKDVGDIADFEIVYDPVKRGFPMHPKLKKA
                     LKENPSAKEVYESLRPSLQLEINRYLSFLKTEESVDRNVIRAIQFLLGKGRFIGRDKP
                     "
     gene            650..1105
                     /locus_tag="PLT08_04870"
     CDS             650..1105
                     /locus_tag="PLT08_04870"
                     /inference="COORDINATES: protein motif:HMM:NF020397.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1801 domain-containing protein"
                     /protein_id="HNE03826.1"
                     /translation="MKTTTASPKGKSTTPTTSVKEFIASLDDEQSKADTHTLIEMMQR
                     ISGHAPKMWNVGTIGFDTYHYKYESGREGDSQTIGFYPRKGKLTVYLMDGTVRYSELL
                     SRLGKHTTSRVCIYIKRLSDIDLSILEQIVRQSYEYVKSQDGHMHRALE"
     gene            1147..1608
                     /locus_tag="PLT08_04875"
     CDS             1147..1608
                     /locus_tag="PLT08_04875"
                     /inference="COORDINATES: protein motif:HMM:NF018184.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HNE03827.1"
                     /translation="MKTMGLKIIGIAVRTTNKDGQSARDIGELWGQFYSQNLLETIPN
                     KLSNDIYSIYTDYKSDFTDEYTTIIGLQVSSLDTIPSGLIGRQFPTETFEVFTAKGEM
                     PQAVMNTWLDIWQRDNELQRKYTYDFEFYGEKSQNGENSEVQIFIATNRTQ"
     gene            1809..2186
                     /locus_tag="PLT08_04880"
     CDS             1809..2186
                     /locus_tag="PLT08_04880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE03828.1"
                     /translation="MITKLFSDGVYGDADKSLRAAQVYYRQAQIKFPPIERPPFRETP
                     LRNNTSGYNGICETYCRTRKGEKISCWNVSWYNPPNKLNSKKFYFHDPQERKQALKEA
                     IQFRKEREAEILKRSRSRRKIIS"
     gene            2318..2821
                     /locus_tag="PLT08_04885"
     CDS             2318..2821
                     /locus_tag="PLT08_04885"
                     /inference="COORDINATES: protein motif:HMM:NF017015.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HNE03829.1"
                     /translation="MNSLQNESLLTKLIEHNNWANLQIIQACSVLNDKQLDASPQSTT
                     KGSIRSTLLHLVASQRGYLSMLTQAEEAQSQTPLTFAELQASAEISGAGLLAVARDEA
                     TMSVKTKIQAKDGFIIVPWVIMVQVINHATEHREQIKSMLSDLGVLPPKIDGWSYGEF
                     TNAYTER"
     gene            2835..3320
                     /locus_tag="PLT08_04890"
     CDS             2835..3320
                     /locus_tag="PLT08_04890"
                     /inference="COORDINATES: protein motif:HMM:NF024698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNE03830.1"
                     /translation="MTNELLLRDVSLDDLPIFFEQQRDEEANTMAAFTAKDPNDREAF
                     FAHWRRILADETIINKTIVVHGQVVGSVASYIDEGNPEVTYWLGKEYWGKGIATKALN
                     EFLAHHNQVRPIYARVAKDNLGSRRVLAKCGFTVIGESKGFANARGQEIEELLLERSA
                     A"
     gene            3384..5063
                     /locus_tag="PLT08_04895"
     CDS             3384..5063
                     /locus_tag="PLT08_04895"
                     /inference="COORDINATES: protein motif:HMM:NF024986.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HNE03831.1"
                     /translation="MANPEHLEALKQGMEKWNTLRSKNFDTVFDLSGADLSGMNLVGI
                     RLYNVNLSGANLSNAYLRNADFSSSDLSGANLTGADLSQSYMFGVKLSDANLEGAIFE
                     GVNADNEVLKLISQSTANPEHLKILKQGVSAWNAWRQENPRVTPILSNADLHELTDMT
                     RVNLSGVIMHGVNLAGVNLESAEFNHANLSEANLAGVNLRTASLTGANLSKANLHGAN
                     LRGVSLIQADLSEADLSNANLDVIPDRDGDTEAFLSYATFRATNLSGASMRKAILRYS
                     NLVDANLTGANLTDADLYNADLVRSNLTNAILTGSDLSLADLVETNLTNAEISGCRIY
                     GASAWNVTLENTSQKDMVISRKDEPIVTVDDLEVGQFIYLMLNNTKIRDVINTITSKG
                     VLILGRFADPQRKAVLDGLREKLREFDLLPIVFDFDRPTDKDYTETVQTLAGMSMFVI
                     ADVTSPKSTPLELEATAKQFKIPFIPIIDISVDPRPFAMLVDLQKSFHWVLPTLQYQT
                     KEDLLDDENLKTYIIDRAVAKREELRVAKSQEPEMLLVRKKGTSTPPATAS"
BASE COUNT         1618 a         1168 c         1172 g         1227 t
ORIGIN      
        1 tgagaaagga aaaaagcgga aagaatccgt ctattggcag gcacgttcaa tgtccctgca
       61 tgagctggaa catataacgg ctattgaaac cttactagcc aaagtcaaat aaccatgatc
      121 aagtttagag ccaaaatcga aatcatcggg gtaaaccctt ttgtgttcct tcctgaacac
      181 gcgttgacag aaattttcaa acaggcaaac ataaccaaag gcaagattcc catcaaaatg
      241 aaaattgacg ggcatgagtt cacgcagacc cttataaaat ggcagggagc ctggcgtttg
      301 tatctaaaca tgccgatgcg aaaagcggct caaaaggatg tgggcgatat cgctgacttt
      361 gaaattgtct acgaccctgt aaagcgcggc ttcccaatgc accctaaact caaaaaagcg
      421 ctgaaagaaa acccatctgc aaaagaggtc tacgaatcat tacgtccctc attgcaattg
      481 gaaatcaacc gctatttgag cttcctgaaa acagaagagt ccgtggaccg aaacgttatt
      541 agggcgatcc agttcctctt aggcaaaggt agatttattg gcagagacaa gccataagta
      601 aatttcaatc ttgacgaaat agaacataag ttctaaaatc ttaggactta tgaaaacaac
      661 taccgcttca ccaaaaggca agtccactac cccaaccact tctgtcaaag agttcattgc
      721 ttcgttggac gatgaacaat caaaagcgga tactcatact cttatcgaaa tgatgcaacg
      781 catcagcggg catgcgccca aaatgtggaa tgtaggcacg attgggtttg atacttatca
      841 ctacaagtat gagagcggac gtgaaggtga cagtcagacc attggctttt atccaagaaa
      901 aggcaaacta accgtttacc tcatggacgg cacggtccgc tattctgagt tgctgtctcg
      961 tttgggcaaa catacaacca gccgggtctg tatatatatt aaacggctta gcgacataga
     1021 cctgtcaatc cttgaacaga tcgtgcggca atcatacgag tacgtaaagt ctcaagacgg
     1081 acacatgcat cgggcgctcg agtaaaaaat ctatgcgttt cgcatatcgg tagcggagaa
     1141 aacctgatga aaacaatggg tctcaaaata attggtattg cagttcgaac gaccaataaa
     1201 gatggtcagt ccgctcgaga tattggggag ttatgggggc aattctattc acaaaatcta
     1261 cttgaaacca ttccaaacaa gttgagtaac gatatttact ctatttacac cgactacaaa
     1321 agtgatttca cagacgaata tacaacaatc ataggtcttc aagtcagcag tttggatacc
     1381 attcccagcg ggctgatcgg tagacaattt cctaccgaaa catttgaagt gtttactgcc
     1441 aagggggaaa tgccccaagc ggttatgaat acctggctcg atatttggca acgagataat
     1501 gaattgcaaa gaaaatacac ctatgacttt gaattctatg gagagaaatc tcaaaatgga
     1561 gaaaattcag aagttcagat ctttatagca accaatagga cccaataatc aaaatactgt
     1621 ccaagtaacc gatcaaaacc ccgctaaccg ctctctatat tctacaaaat ttgtcagata
     1681 taattccttt cacatcttac atacgaaagg aatttgataa tcgaatgagt acttactctt
     1741 tgaggggtat cagcaggata cactcaaagg gggctggctg gttagtcagg ttgtatcgga
     1801 atgctaaagt gatcacaaaa ctctttagcg atggcgttta tggagatgcc gataaatcac
     1861 taagggcggc gcaagtgtat taccggcagg cacaaataaa atttccaccc attgaaagac
     1921 ctccttttcg ggaaacgcca cttcggaata atacctctgg ttacaatggc atttgtgaga
     1981 cgtactgccg cactcgtaaa ggcgagaaaa tctcatgctg gaatgtcagt tggtataacc
     2041 cgccaaacaa gttgaacagt aaaaagttct attttcacga tcctcaagaa cgcaagcaag
     2101 ccttgaaaga agcgatccaa tttcgcaaag aacgagaagc cgagatatta aagcgctctc
     2161 gcagtagacg aaaaatcata tcataagggt tgtgagctcg tagccgtcaa aatgatgcac
     2221 aaaaaagttt cgtgccgttt tgacggctat tgttataaat aatatacagg atagcctatt
     2281 tataaataat tgataagtga ggagcaaatc ttagaaaatg aattctttgc agaacgagag
     2341 tcttcttacg aagttaatag aacacaacaa ttgggcaaac cttcagatta ttcaggcgtg
     2401 ctctgttttg aatgacaaac aactggacgc tagtcctcag tctacgacca agggcagtat
     2461 tcgtagcacc ttgttacatc ttgttgcctc tcaacgaggc tatttatcga tgttgacaca
     2521 ggcagaggaa gcacaatctc aaactccgct cacatttgct gagttgcaag cgtctgcaga
     2581 aataagcggc gcaggcttgc tagcagtggc aagagatgaa gcaaccatgt ctgtaaaaac
     2641 aaaaatacag gcaaaggatg gcttcatcat tgtgccttgg gtgattatgg tacaggttat
     2701 caatcacgcc actgaacacc gggagcagat caaaagcatg cttagcgatt tgggtgtatt
     2761 acctccaaag atcgatgggt ggagctatgg cgaatttacg aatgcatata cagaaagata
     2821 ggcaaattta cgacatgaca aacgaacttc ttctgcggga tgttagcctc gatgaccttc
     2881 caatcttttt tgaacaacaa cgagatgagg aagccaatac catggcggca tttaccgcca
     2941 aagacccaaa tgatcgggaa gcctttttcg cccactggcg aagaattttg gcagatgaga
     3001 cgatcatcaa taagaccatc gttgtccacg ggcaggttgt cggcagtgtt gccagttaca
     3061 tagacgaggg caaccctgaa gtcacttatt ggcttgggaa ggaatattgg ggcaaaggca
     3121 ttgcgacaaa agcattgaac gagtttttag cccaccacaa ccaagtccga cccatctatg
     3181 cgcgtgtggc aaaagataac ctcggctcgc ggcgcgtact ggcaaaatgc ggctttacgg
     3241 tcatcggaga atcgaaaggg tttgccaacg cacgcggtca ggagatcgaa gagttgcttt
     3301 tagaacgatc agcggcgtaa gctcatcaaa actgatatca tcgagaaaaa agcaaccact
     3361 ttaaaaagag gagcctatcc cctatggcaa accccgaaca tctcgaagct ctaaagcaag
     3421 gcatggagaa atggaataca ctccgaagca aaaattttga tacggtcttt gatcttagcg
     3481 gcgcagactt gagcggtatg aatttggttg gcatacgttt atacaatgtt aatttaagcg
     3541 gagctaatct gtccaatgcc tatttgcgca atgcagattt ctcatcttca gaccttagcg
     3601 gagccaatct caccggtgcc gatttaagcc agtcctatat gtttggggtg aagctgagtg
     3661 acgcaaattt agaaggtgcg atttttgaag gggtgaatgc agataatgaa gttcttaaac
     3721 tcatcagtca atcaacagcc aaccccgaac atctgaagat tcttaagcaa ggggttagcg
     3781 catggaatgc ctggcggcaa gaaaacccca gagtgacccc cattctgagc aatgctgacc
     3841 tgcacgagtt gaccgacatg acacgcgtca acttgagcgg cgtcataatg cacggcgtta
     3901 acctcgccgg ggtcaacttg gaatcggcag agtttaacca tgccaactta agcgaagcga
     3961 accttgccgg ggtcaactta cgaaccgcat ccttaaccgg agcgaacctg agcaaagcaa
     4021 atttacatgg ggcaaatttg cgaggcgtgt ccttaatcca ggcagacctc agcgaagcgg
     4081 atttgagcaa cgccaatttg gatgtcatcc cagatcgtga tggcgatacg gaggcgttcc
     4141 tatcttatgc tacgttcagg gcaaccaacc ttagcggagc cagcatgcgc aaagccattt
     4201 tgcgctactc aaacttagtg gatgcaaact tgaccggagc caacctgacc gatgccgacc
     4261 tctataatgc cgatttggtc cggtcgaacc tcaccaacgc gatactcacc ggctctgacc
     4321 tcagcctggc tgatttggta gaaaccaact taaccaacgc tgagatctca ggttgtcgaa
     4381 tttatggggc ttcagcatgg aatgtcactt tggagaatac ctctcaaaaa gacatggtca
     4441 tttcgcggaa agatgaaccg attgtcaccg tggatgatct ggaagttggt cagttcattt
     4501 acctgatgct gaacaatacc aagatccgag atgtgatcaa taccatcaca tctaaaggtg
     4561 tactcattct tggcaggttc gccgacccgc aacgcaaagc cgttttggat ggactgcgag
     4621 aaaagctacg cgagttcgac ctattgccga tcgtcttcga ttttgaccgt cccaccgata
     4681 aagactatac cgaaacggtt caaaccctgg caggtatgag catgttcgtc atcgccgatg
     4741 tgaccagccc gaagtcgacc ccattagagt tggaagctac cgccaaacaa tttaagatcc
     4801 ctttcattcc cattatcgat atcagcgtag acccacgtcc gtttgccatg ctggtcgatt
     4861 tgcaaaaatc tttccattgg gttttgccaa cccttcaata tcaaaccaaa gaagacctgt
     4921 tggatgatga gaatctaaaa acctatatca ttgatcgcgc cgttgcaaaa cgcgaagagt
     4981 tacgagttgc aaaaagccag gagcctgaaa tgctgctcgt tcgcaaaaaa ggcacatcca
     5041 ccccgcctgc aacagcaagt tgaggcagta aaccattaca ggagaaaacc atgtcgtttc
     5101 aagcctattt agataaagtt gaagaactta caggcaaagc cccgaaagaa ttcgtggctg
     5161 aggcaaagaa aaagaagctg acaac
//
LOCUS       DAOQJE010000131         5166 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_9399_length_5166_cov_6.919977, whole genome shotgun sequence.
ACCESSION   DAOQJE010000131 DAOQJE010000000
VERSION     DAOQJE010000131.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 5166)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5166)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5166
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9399_length_5166_cov_6.919977"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            192..1115
                     /locus_tag="PLT08_04900"
     CDS             192..1115
                     /locus_tag="PLT08_04900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNE03832.1"
                     /translation="MIKVSGLTKDYGSRRAIHNLSFDAQQGEIVGFLGPNGAGKTTTM
                     RILTGYMPPTDGEAIVAGYDVVEESLEVRKRVGYLPESVPLYYDMVVSEYLKYMGELR
                     RIPNVDDRVDEVLDMVGLFDRADGYIGNLSKGMKQRVGLAQALLHRPEVLILDEPTIG
                     LDPGQVVEVRELIREIGKERTVLLSTHLLNEAQNLCDRVLIINKGKIVAEDTTENLQA
                     RLLGAERVVIRVRGEAEELADSIKSIKGVRKVETKDDGAVEFEFASGKDLRPEVAKQV
                     IKDGYDLLELRPLGMSLEEIFLELTGNEGKK"
     gene            1120..1863
                     /locus_tag="PLT08_04905"
     CDS             1120..1863
                     /locus_tag="PLT08_04905"
                     /inference="COORDINATES: protein motif:HMM:NF024141.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNE03833.1"
                     /translation="MNNIWTIAKREYNNYFNGPLAYVVMLAILLPLGIYFSLILFYSS
                     QQGLYGGSAAPDITPVNGLFAFLMIFLSPALTMRLLSDEARMGTLELLLTAPVRDIEL
                     VVGKWLGAMLFILTLLGTTLIYPLVLNNFVDPGIDWMVVLSSYIALILLCGTMLALGV
                     GISAIFTNQFAAFFVTLGLIFFLYFMIGLPAGLLPQSGEVFNYLSIQNHFDQMNSGTL
                     TLGGIVYNLSLTVFGLFAGFTAIETRRWR"
     gene            1863..3485
                     /locus_tag="PLT08_04910"
     CDS             1863..3485
                     /locus_tag="PLT08_04910"
                     /inference="COORDINATES: protein motif:HMM:NF021351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GldG family protein"
                     /protein_id="HNE03834.1"
                     /translation="MAGKKKNPNARYAVIALAVSLVACISTGLIGSANLLSNIGMFPL
                     SDEIQNGVNIALQVSIGLLIIALAVAAILNPDPVRRFFTGRQARYGSNSLILTIAVIG
                     ILIAGNYIVFKNTDLLGSPYDFTEDKSNTLAPETLQILAELKEPVVATGFFSTNSSTA
                     SAEELLAKFKNNSDGKFTYNFVNPDVDPVAAREAGITGDGKIMLQMGETKEIATFATE
                     DELARTLLRLMSPGARTVYFLQGHGEISLESGNELAYSVVQSTLEAKNYTVSTLNLLT
                     TREIPADALAIIVAGPQKPVSAEEVDLLKAYVDKGGSLVVLEDPIFFTKFGDAADPLA
                     EYLVNDWGIVLNNDVVIDGVNQQNPLAAISSLYNPDHPITQNLTENLFVIMPQARSIS
                     VSGEKENITQTWLISTTENSWGETSPLVENENPQFDPATDVQGPLYLALAAENSATTG
                     RVVVFGNSLFAIDGNFDVYGNGNFFINSVDWAAEQEDLLNLTTRPRTERIFTPPVETW
                     RFLLLVLVIVIIIPGMVVFFGVSAWLARRKRG"
     gene            3489..4211
                     /locus_tag="PLT08_04915"
     CDS             3489..4211
                     /locus_tag="PLT08_04915"
                     /inference="COORDINATES: protein motif:HMM:NF025598.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4340 domain-containing protein"
                     /protein_id="HNE03835.1"
                     /translation="MATKSTKTTKSTKTTKTTKPRASRAQSTPKVSKPAVRTGTWVAL
                     IAFIAVIATLLYMNRKAETEATATETPAAEETFIFSSESVVSSIEVKPAEGNAVKVER
                     NEEKTWVLSQPEKAEANQAASEAAATQIGALSIVTTIEGNKDPSIFGLDAPVYTITVT
                     FDDGKKGVLEVGDTTPTSTGYYVRLDKDKVYVVAMDGIDALTSLTVAPPYLNTPTPVP
                     TATSTPLPTDTPATTTEATPTP"
     gene            4392..4586
                     /locus_tag="PLT08_04920"
                     /pseudo
     CDS             4392..4586
                     /locus_tag="PLT08_04920"
                     /inference="COORDINATES: protein motif:HMM:NF015912.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor region1.1
                     domain-containing protein"
     gene            4707..5166
                     /locus_tag="PLT08_04925"
                     /pseudo
     CDS             4707..5166
                     /locus_tag="PLT08_04925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176834.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
BASE COUNT         1267 a         1360 c         1244 g         1295 t
ORIGIN      
        1 attatacaag atggaatgaa ttggtcagac aaaattagga ttggattaga gggctataca
       61 cccgcttgac tttggtcttt catatattgt atagtattct gcggtacgaa aaaaaaggca
      121 ccgccaacac ggctgtgtct tttctttgcg ataatcccat tttatatatt atttcaccgt
      181 gaggagaagc aatgatcaaa gttagtggtc tcacaaaaga ctatgggtca cgtcgcgcca
      241 tccacaatct gagttttgat gcccaacagg gcgagattgt gggttttttg ggtcccaatg
      301 gcgccgggaa aaccaccacc atgcgcatcc tgactggtta catgccgcct actgatggcg
      361 aagcgattgt agctggttat gatgtggtgg aagaatcgtt ggaagtacgc aagcgcgtcg
      421 gctatttgcc ggagtctgtg ccgctgtact acgacatggt cgtttctgaa tacttaaagt
      481 atatgggcga acttcgccgc atcccgaatg tggatgaccg tgtggatgaa gtgttggata
      541 tggtcggctt gtttgaccgt gccgacgggt acattggcaa cctatccaag ggtatgaaac
      601 agcgtgtggg cttggctcag gcgcttttgc accgccccga agtgctcatc ctcgacgagc
      661 cgacaattgg tctcgaccct gggcaggtgg tagaagtccg cgaattaatc cgcgagattg
      721 gtaaagagcg cacggtgctc ctctccactc accttttgaa tgaagcgcaa aacctctgcg
      781 accgcgtctt gatcatcaac aagggcaaga tcgtcgccga agacaccacc gaaaacttgc
      841 aggctcgctt gctcggtgcc gaacgggttg tgatccgtgt acgtggcgaa gccgaagagc
      901 ttgcagactc gatcaagagc attaagggtg tccgcaaggt ggaaaccaaa gacgacggtg
      961 ctgtggaatt cgagtttgcc tccggcaagg atttacgccc cgaagttgcc aaacaggtta
     1021 ttaaagatgg gtacgacctt ttggaactcc gcccgttggg catgagcctc gaagaaatct
     1081 tcctcgaact cactggaaac gaagggaaga aataatccca tgaacaatat ttggaccatc
     1141 gcaaaacgcg aatacaacaa ctactttaac ggaccgctcg cctatgttgt gatgctggcg
     1201 atccttttgc cacttggcat ttacttcagt ttgatccttt tctacagcag ccagcaaggt
     1261 ttatatggcg gcagcgctgc ccccgatatc actcccgtca acgggttgtt tgctttcctt
     1321 atgatcttcc tcagcccggc gcttactatg cgtttgctct ctgacgaagc tcgtatggga
     1381 actctcgaac ttttgctgac cgcccccgtc cgcgatattg agctggtggt tggcaagtgg
     1441 ctgggcgcca tgttgttcat cctcaccttg cttggcacta ccttgatcta tccgctggtg
     1501 ctgaacaact tcgttgaccc cggcatcgac tggatggtgg tgctttcttc ttatatcgct
     1561 ttgatcctgc tttgcggcac catgttggct ttgggtgttg gcatctctgc cattttcacg
     1621 aaccagttcg ctgccttctt cgtgaccctc ggcttgatct tcttcttgta cttcatgatc
     1681 ggtctgcctg cagggttgct cccccaaagc ggtgaagtct tcaactactt gagcattcaa
     1741 aatcactttg accaaatgaa ttccggcaca ctaacactcg gcggcatcgt ctataacttg
     1801 agcctgacgg tgttcggtct cttcgccggt tttaccgcaa ttgaaactcg gagatggcga
     1861 taatggctgg gaaaaagaaa aatccaaacg caagatacgc agtcatcgcg ctggcagttt
     1921 ctctggtcgc atgtatttcg accggtttga tcggctctgc gaatctgctt tctaacattg
     1981 gcatgttccc gctttctgac gaaattcaga acggtgtgaa cattgcttta caagtcagca
     2041 ttggtttatt gatcattgct ctggcagtcg ccgcgatttt gaacccggat cccgtccgcc
     2101 gcttctttac cggacgccaa gctcgctatg gttcaaattc cttgatcctc acgattgccg
     2161 tgatcggtat cctcattgct gggaactaca ttgtcttcaa gaacaccgac ttgcttggtt
     2221 ctccttacga ttttaccgaa gacaagagca atacccttgc tccagaaacc ctgcaaattt
     2281 tggctgaatt aaaagaacct gtcgtagcta caggcttttt ctcgacaaat tcaagcacag
     2341 ccagcgcaga ggaattgctc gctaaattca agaataacag cgacggcaag tttacctaca
     2401 acttcgtgaa cccagatgtt gaccccgttg ccgctcgcga agctggtatt acaggcgatg
     2461 gcaagatcat gttgcaaatg ggcgaaacaa aagaaatcgc tacctttgct acagaagatg
     2521 aacttgcgcg cacccttttg cgccttatga gccccggcgc acgtacggtc tatttcttgc
     2581 agggacatgg cgagatcagc cttgaatctg gcaacgaatt agcctacagc gttgtgcaaa
     2641 gtacccttga ggcaaagaat tacaccgtca gcacgcttaa tctgttgacg actcgcgaaa
     2701 tccctgcaga cgctttggca attatcgttg cgggtccgca aaaacctgtc agcgcagaag
     2761 aagtggactt gctcaaagcc tatgtggata agggcggttc cttggtcgtc ctcgaagacc
     2821 cgatcttctt caccaagttt ggtgatgccg cagacccctt ggctgaatac ctcgtcaatg
     2881 attggggcat tgttctcaat aatgacgttg tgatcgatgg tgtcaaccag caaaatccgc
     2941 tggcggcgat ctcttctttg tacaatcccg atcaccccat cacccaaaac ctgaccgaaa
     3001 accttttcgt catcatgcca caagctcgca gcatcagtgt ttctggtgaa aaagaaaata
     3061 tcacccaaac ttggctcatc agcaccacag aaaactcctg gggcgaaacc tctcccttgg
     3121 tagaaaacga gaatccacaa ttcgaccccg caactgacgt tcaaggtcct ttgtacctcg
     3181 cccttgctgc ggagaattct gcgacgaccg gtcgtgtggt tgtgttcggt aactcacttt
     3241 ttgctatcga cggaaacttc gatgtgtatg gcaacggcaa cttcttcatc aattctgtag
     3301 attgggctgc cgaacaggaa gaccttctga acctcaccac ccgtccacgc accgaacgca
     3361 tcttcacacc tccggttgaa acctggcgct tcctgctctt ggttctcgtg atcgtgatta
     3421 tcatccccgg tatggttgtt ttctttggcg tgtctgcatg gcttgcccgt cgtaaacgag
     3481 gttaattcat ggctacgaaa tcgaccaaaa caacaaaatc tacaaaaaca accaagacca
     3541 ccaaaccacg cgcgtctcgt gcgcaaagca ccccaaaggt ctccaaaccc gccgtccgca
     3601 ccggaacatg ggttgcgctg atcgctttca tcgctgtgat tgccacttta ctgtacatga
     3661 atcgcaaggc agaaacagaa gctaccgcta cagaaacccc ggcggctgaa gagactttca
     3721 tcttctcaag tgagagcgtt gttagcagta ttgaggttaa gcctgctgaa ggtaacgcag
     3781 ttaaggttga acgaaacgaa gagaaaacct gggtgttatc tcagcctgaa aaagcagaag
     3841 caaatcaggc tgcgtctgag gcggctgcca ctcaaattgg tgcgctgagc attgtcacca
     3901 ccattgaagg caataaagac ccctccatct tcggtttgga tgccccggtt tacaccatca
     3961 cagtcacctt tgatgatggt aaaaaaggcg tcctcgaagt gggcgatacc accccaacct
     4021 ccactggtta ctacgtgcgt ttggataaag ataaggtgta tgttgtcgct atggacggaa
     4081 ttgacgcgct caccagcctg accgtcgccc cgccatattt gaataccccc acaccggttc
     4141 caacggcgac atccacgcct cttccgaccg atacgcctgc tacaacaacc gaagctactc
     4201 ctacccccta agtttttacg gcgtcgggga atctgtttga aaattgaggc agattccccg
     4261 acccgattcc tccaaaaagt aatacaaaat cattgcaagg catctgataa gccgaacact
     4321 attgcttttc ggttcaaaaa cgtgtattct gaaatgtaag acaaatattt gttgaaagtt
     4381 ggatgatgtg aatggatgta gataagatac taattagcga agatgatgaa gaagagtatt
     4441 ctgctatagc gcgtttgatc gaactggggc ggcaaaaatc ctttgtaact ctcgacgaca
     4501 tattgcactt ctttcccgaa gcggaacaag acgtagaaca gcttgaagaa gcgttctctg
     4561 cgcttttaag cgctggtatc ccgttcgtgg aagaaagcat gacccccgaa cccaccgaag
     4621 acgaattggt ggctgtagaa gagagcggtg aagctgaacc tgaacccgat ctcgcgctgg
     4681 atgattacct agccaatatc gatactgacg ataccattgg tctgtacctc aaagaggtca
     4741 gccgtgtgcc gcttcttacc gccacggaag aagttcaact tgctcagcgc atcgaacgcg
     4801 gacgttctgc ccgcgaagaa cttgcgcatg ggtctgtttc tcccaagcgt cgcctagaac
     4861 tccgccgttt tattgaagac ggctgggcag ctcgtgaaca ccttattacc gcgaactctc
     4921 gcctcgtgat ctccgtcgcc aagaaataca tgggacgcgg tgtgcccttc ctagatctta
     4981 ttcaggaagg caatatcggt ttgatccgcg caaccaagaa gttcgactat cgccgtggtc
     5041 acaagttctc gacctacgcc acctggtgga tccgccaagc tgtgacccgt gccatcgccg
     5101 atcagggacg taccatccgc gtaccggttc acatgggcga ccaaatcaac aagctcctgc
     5161 gcgttc
//
LOCUS       DAOQJE010000132         5158 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_9421_length_5158_cov_6.722712, whole genome shotgun sequence.
ACCESSION   DAOQJE010000132 DAOQJE010000000
VERSION     DAOQJE010000132.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 5158)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5158)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5158
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9421_length_5158_cov_6.722712"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(58..1458)
                     /locus_tag="PLT08_04930"
     CDS             complement(58..1458)
                     /locus_tag="PLT08_04930"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HNE03836.1"
                     /translation="MRKRFFIALLLLTLIVPFKPALAQDTGPIYIVQPGDSLYSIAAR
                     FSISLDELLAANGIADANSLAVGQQLIIPGLDGITGILDTKFINFGDSYRGLMRQTQV
                     QAPLFRKLNHVVSPSEFYVGSNMIVPVQDQTQYSKRISPTTGESMLELAVKNNTDVWS
                     LSSINQLSGSWDVLPGDTLYAPGEASGNNASGLPSAFVSAEIRDLPIKQGGTGVITVV
                     AQPGVTLSGILVDHQLHFFPQEDGSQVALQGVHALLEPGVYPLRLDATLPDGSTQSYE
                     QLILIISGNYPEDPLLYVDPATIDPASTEPELQQLVTITTPTSPTKLWAGSFISPAIA
                     YAESTYFTSRYGNRRTYIGQGTDLTVQGFHTGLDFGGGDGLPITAPANGVVVFAGPWT
                     VRGNATVIDHGWGIYSGFWHQSAIQVQVGQTVNQGDVIGLVGGTGRVTGAHLHWELWV
                     NGVQVDPLDWLNQDYP"
     gene            complement(1451..1822)
                     /locus_tag="PLT08_04935"
     CDS             complement(1451..1822)
                     /locus_tag="PLT08_04935"
                     /inference="COORDINATES: protein motif:HMM:NF014678.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF192 domain-containing protein"
                     /protein_id="HNE03837.1"
                     /translation="MPRPITIQNLSRTLEKPATVGYCDSFQCKLRGLMFRSHLDINDG
                     LLLVEKRNSRLDTSIHMFFVPFDLTVVWINSDLTVVDKVLAKSWKPAYFPKADAQYTL
                     EIHPDRWADYEIGDKVEFKNA"
     gene            complement(1875..2261)
                     /locus_tag="PLT08_04940"
     CDS             complement(1875..2261)
                     /locus_tag="PLT08_04940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNE03838.1"
                     /translation="MDETPTKTILCVEDEPEMIDLIRLILSRKGYSVKGASGGVEGIR
                     LIKELHPDLVLLDLMMPDMDGWEVYQQMKAEASLREIPVIVVTAKAQNIDKVLGLHIA
                     KVDDYIAKPFSPQELVDSVDKVFSHK"
     gene            2313..3077
                     /locus_tag="PLT08_04945"
     CDS             2313..3077
                     /locus_tag="PLT08_04945"
                     /inference="COORDINATES: protein motif:HMM:TIGR00633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HNE03839.1"
                     /translation="MNIITWNVNGIRAALGKNALDWAFEKKPDALCLQEVKARPEQLN
                     EEQAAQLKLPYIWNPAERPGYSGVATFYKTHPDETVLGMDAPEFDVEGRIIQTRWGNV
                     RLFNIYFPNGQRGHDRVDYKLRFYARLLEICNTLHSKGELVVITGDFNTSHMPIDLKN
                     PKANEKTSGFMPEEREWVQKYLDNGFADAYRRLYPDRVQYTWWTYRLDARARGIGWRL
                     DYFLVSEALMPRVKDVIIHEQVMGSDHCPVELVLNV"
     gene            3277..5136
                     /gene="ftsH"
                     /locus_tag="PLT08_04950"
     CDS             3277..5136
                     /gene="ftsH"
                     /locus_tag="PLT08_04950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HNE03840.1"
                     /translation="MNSRSQSFFVYFLLLVAIGAMIFVGLRDNSSAAEPLTISEVARM
                     IQGGQVARIIIESDDTIRVVKVNGSEETALESRKESNTTLIEQLRDYGVTPEQLAEMN
                     IEVSAPSPWAGVLSGALYLLPVLFMGGLLWFIFRQAQGSNNAAMSFGKSRARMFSGEH
                     PTVTFADVAGADEAKQELAEIVEFLKEPQKFIQLGARIPKGVLLVGPPGTGKTLLSKA
                     VSGEAGVPFFSISGSEFVEMFVGVGASRVRDLFDQAKRHSPCIIFVDEIDAVGRQRGA
                     GLGGSHDEREQTLNQMLVEMDGFDTDTNIIIIAATNRPDILDPALLRPGRFDRRVTLD
                     RPDVKGREAILKVHVKGKPLEPQADLASVARGTPGFAGADLENLVNEAAILSARRNKK
                     SIGQPELEEAIERVVMGPERKSRLISDEEKRIIAYHEAGHAIVANAIAEADPVQKVTI
                     VGRGQAGGVTWFRPDEDRMLMSRKKMLATLAYALGGRAAEELVFDDITSGASGDIEQV
                     TRIARAMVTRLGMSAEMGTMVYGQKEELIFLGREISEQRDYSDAVAEQIDREVRKLVE
                     DAYKQAKATVKKYRKQLDAVAQKLLEVESITREEFESIFPPPLGKKSGTPQVA"
BASE COUNT         1309 a         1300 c         1298 g         1251 t
ORIGIN      
        1 agattattca tgtcggaaag tttagcatat tagaggatga agtgcaacgc ctcgaaccta
       61 cgggtaatcc tgattcaacc agtcaagcgg gtccacttgg acgccgttta cccacaactc
      121 ccaatgcaag tgagccccgg tcacacgccc tgttccgcca accagaccaa tcacatcgcc
      181 ctggttgacc gtttgcccca cctgtacctg tatagcagac tgatgccaaa acccactgta
      241 aatgccccag ccatgatcaa tgacggttgc atttccacgc accgtccacg gaccggcgaa
      301 aacgacgacc ccatttgccg gagccgtaat gggcaaccca tcacctccac caaaatctaa
      361 ccctgtgtgg aagccctgca cagtcaggtc tgttccctga ccaatatagg tgcgacggtt
      421 gccataccgc gaggtgaaat acgtcgattc agcgtaggca atggctggag aaataaatga
      481 accagcccac agcttagtcg gactcgttgg cgtcgtaatg gtgacaagtt gctgaagttc
      541 aggttccgta gaagccgggt caatcgtagc ggggtctaca tagagaagcg ggtcttcagg
      601 gtaattgcca gagataataa gaatcaactg ctcgtacgat tgtgtactgc catcgggcaa
      661 cgtggcatcc aagcgtaaag gatacacacc cggctcaagt aaagcatgca ctccttgtaa
      721 ggcaacttgt gagccatctt cttgaggaaa gaaatgcaat tgatggtcga ccaaaatacc
      781 gctgagtgtg acacctggtt gcgctactac tgtgattacg cctgtgcccc cttgcttaat
      841 gggcaggtcg cgaatctctg cgctgacaaa tgccgacggc aatccgctgg cattgtttcc
      901 actcgcttca ccgggagcat acaaagtatc tccaggtaga acatcccaac tgccacttaa
      961 ttgattgatc gaagataacg accaaacatc cgtattattt ttgactgcca attccagcat
     1021 agactcgccc gtagtagggg agatgcgctt gctgtattgg gtttgatctt gtacaggcac
     1081 gatcatatta ctgcccacat aaaattcact gggactcacc acatggttga gtttgcggaa
     1141 caacggagct tgtacctgcg tctggcgcat taagccgcga tacgaatcac caaaattaat
     1201 gaatttggta tctaaaattc cggtgatgcc atctaacccg gggatgatca gttgttgccc
     1261 aacagccaac gagttggcat cggcaatccc atttgcagct aagagttcat cgaggctaat
     1321 gctgaagcgt gccgcaatcg aatacagact atctcccggt tggacgatgt agatcggtcc
     1381 ggtatcttga gcaagcgctg gtttgaaggg aacaatgagg gtgaggagca gaagagctat
     1441 gaaaaaacgt ttacgcattt ttgaattcaa ctttatcgcc aatctcataa tccgcccaac
     1501 ggtctgggtg tatttccaac gtgtattgtg catctgcctt ggggaagtag gcgggcttcc
     1561 atgattttgc aagaacctta tccaccaccg tcaggtcgga gttgatccag acgacggtaa
     1621 ggtcaaatgg cacaaagaac atatgaatgg aagtgtcgag ccgcgagttt cgtttttcaa
     1681 cgaggagcaa gccatcgtta atgtccagat gcgagcggaa catcaatccg cgcagtttgc
     1741 actgaaacga gtcacaatag cccacagtgg cgggcttttc caaggttcga ctaaggtttt
     1801 gtatggtgat ggggcgaggc atggggtttt atgaaaaatg ggcggcgtat tcgccgcccg
     1861 ttttggaatg aaaattattt atggctaaaa actttatcga cgctatccac taactcttgt
     1921 ggactaaacg gcttggcaat gtagtcatcc actttggcga tgtgtaaacc aagaacttta
     1981 tcgatatttt gtgctttggc agtgacaaca ataacgggga tttcgcgcag agaagcttcg
     2041 gctttcattt gttggtatac ttcccaaccg tccatatcgg gcatcataag atcaagcaaa
     2101 acaaggtctg gatgaagttc tttaataagg cgaattcctt ccacaccgcc agacgcgcct
     2161 tttacgctat acccctttcg agaaagaatc aaccgaatca gatcgatcat ttcgggttca
     2221 tcttcaacgc ataggatggt tttggtaggt gtttcatcca taatttttgc cttctaaaat
     2281 aagtttacca taaagagacc aaccaacttc gtatgaatat cattacctgg aatgtgaacg
     2341 gaattcgcgc tgcacttggc aagaatgctc ttgattgggc gttcgaaaaa aagccagatg
     2401 cgttgtgttt gcaggaagtt aaagcccgcc ctgaacaact aaatgaagaa caagccgctc
     2461 aattgaagct gccttacatc tggaatcctg ccgaacgccc agggtatagt ggggtagcga
     2521 ctttttataa gacacatcca gatgaaactg tgcttggaat ggacgcgcct gaatttgatg
     2581 tagaagggcg catcatccaa acccgttggg ggaacgtacg tctcttcaat atttatttcc
     2641 caaatggaca acgcggacat gaccgcgtgg actataaatt acgcttttat gcccgtttgc
     2701 ttgaaatatg taacacgctt cattctaagg gagagctggt cgtcattaca ggcgacttca
     2761 acacctctca catgcccatc gacttgaaga atcccaaagc caacgagaag acttctgggt
     2821 ttatgccaga agaacgcgag tgggttcaaa aatatctgga taacggcttt gccgatgcct
     2881 accgccgcct ctacccagac cgggtccaat atacttggtg gacctatcgc ctcgatgctc
     2941 gcgcccgcgg aattggctgg cgcttggatt atttcctcgt ctcagaggcg ctcatgcccc
     3001 gcgtaaaaga tgtcatcatt catgagcagg tgatgggctc tgatcattgt ccggtagagc
     3061 tggttttaaa tgtgtaaact ttgtggatgg ctgattaatt tctaataagt tccgttgaaa
     3121 ttatccccta gggtgacaaa agattgattg gttttgcaag caggttccgg ttagactttg
     3181 aaccgtgtta gaaaatcgta ataataaaag cccagacggt atctctactg gtatggcttg
     3241 gtataatgac cgcgtacgtc aaatttggag gattccgtga attcccgtag tcaatcgttt
     3301 tttgtgtact ttttattgct cgtagccatc ggagcgatga tttttgttgg cttgcgagac
     3361 aattccagcg ctgccgagcc gctcaccatc agtgaggtgg cacggatgat tcagggcggg
     3421 caagtggcac gcatcatcat tgagagcgac gataccattc gtgtggtaaa ggtcaatggg
     3481 tctgaagaaa cagcccttga atcccgcaaa gagtcaaata cgacccttat cgaacaactc
     3541 cgtgattatg gtgtaacccc cgagcaactt gctgaaatga atatcgaagt cagcgcacca
     3601 tcgccatggg cgggtgtgtt gagcggcgct ttgtatctgt taccggttct gtttatgggt
     3661 gggttgcttt ggttcatttt ccgccaggca caaggcagca acaatgcagc gatgtccttt
     3721 ggtaaatctc gtgcgcgcat gttcagtggg gaacatccca ccgtcacctt tgccgacgtt
     3781 gccggcgccg acgaagccaa acaggaattg gcagagatcg tcgagttcct gaaagagccg
     3841 cagaagttta tccaacttgg tgctcgtatt ccgaagggcg ttttgttggt gggccctccc
     3901 ggaaccggta aaacccttct ttctaaagct gtttctggtg aagcaggcgt gcccttcttc
     3961 tccatctctg gttctgaatt cgtagaaatg ttcgtaggtg tgggcgccag ccgtgtgcgt
     4021 gacctgttcg accaagccaa gcgccattcg ccttgtatta tctttgtaga tgaaattgat
     4081 gcggtgggtc gccaacgtgg agcaggactc ggcggttcac acgatgaacg cgagcagacc
     4141 ctcaaccaga tgctcgtcga aatggacggc ttcgataccg acaccaacat catcatcatt
     4201 gcggcaacca accgccccga tattctggac ccggctcttc tccgccccgg tcgctttgac
     4261 cgccgcgtga cgcttgatcg ccccgatgta aagggacgtg aagccatcct caaggtgcac
     4321 gttaaaggca aacccttgga accgcaagcg gacctcgctt ctgtggcacg tggtacaccc
     4381 ggtttcgccg gtgccgacct tgaaaatctt gtcaacgaag cggcaatttt atccgctcgt
     4441 cgcaataaga aatccatcgg tcaacccgaa ttggaagaag ctatcgaacg cgtggtgatg
     4501 ggaccggaac gcaagagccg cctcatctct gacgaagaga agcgcatcat tgcctaccat
     4561 gaggctggtc atgccatcgt agccaatgcc atcgctgaag ccgaccccgt ccaaaaagtg
     4621 accatcgtcg gtcgcggaca agctggcggc gtgacttggt tccgccccga tgaagaccgc
     4681 atgctcatgt ctcgcaagaa gatgcttgcc accctggcct atgcgctcgg tgggcgcgcc
     4741 gccgaagaac tcgtcttcga cgacatcacc tctggtgcct ccggcgatat tgaacaggtg
     4801 acccgaattg cccgtgccat ggtcacccgc ctcggcatga gcgctgaaat gggcaccatg
     4861 gtctacggcc aaaaggaaga gttgatcttc ctcgggcgtg aaatttccga acaacgcgat
     4921 tattccgacg ccgttgccga acagatcgac cgtgaagttc gcaaattggt cgaagacgcc
     4981 tacaaacaag ccaaggcaac cgtgaagaaa tatcgcaagc aattggatgc cgtggcccag
     5041 aaactccttg aggttgagtc catcactcgc gaagagtttg aatccatctt cccgccgccg
     5101 cttggcaaga aaagcggtac tccgcaagtc gcataataat gataaaagta ccttctgc
//
LOCUS       DAOQJE010000133         5157 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_9425_length_5157_cov_6.078793, whole genome shotgun sequence.
ACCESSION   DAOQJE010000133 DAOQJE010000000
VERSION     DAOQJE010000133.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 5157)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5157)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5157
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9425_length_5157_cov_6.078793"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..835)
                     /locus_tag="PLT08_04955"
     CDS             complement(<1..835)
                     /locus_tag="PLT08_04955"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012424.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoamide acetyltransferase family
                     protein"
                     /protein_id="HNE03841.1"
                     /translation="MATKVYVPRLGEGVDEVTVTKWLKQVGDSINELEPLLEVNTDKV
                     DTEIPAPATGTLLQIVAQEGVPAKVGELLAIIGKPGETVDSGQASIESVASSPTPVTQ
                     QSPAVIPQPQSINRDLGFISPVVAKIAAEHGVDLSQVNGTGLNGRITKNDVLNFVESG
                     KSKGTGQPSNISTFKPSNLTPDTGTLGTLIKHTTMRKSIAQHMVESKHTSPHVLTVME
                     ADMSRVAKHRAANKAAFERDGVNLTFTAYFMMAIVAGLKAYPQTNSSWTDEGLLIHKN
                     VN"
     gene            complement(860..3280)
                     /locus_tag="PLT08_04960"
     CDS             complement(860..3280)
                     /locus_tag="PLT08_04960"
                     /inference="COORDINATES: protein motif:HMM:NF012880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016624 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, disulfide as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HNE03842.1"
                     /translation="MPNKKKGTIALPLEKDEIIKDYRLAYQSRQASLIGRREVLSGKA
                     KFGIFGDGKEIAQLAIARAFQKGDWRSGYYRDQTWMFMLGIMSIQEFFAQLYAHADAQ
                     YDPASGGRQMNAHFATRHLHPNGAWRAQTQLYNTSSDVSCTAAQMPRAVGLAYASVLY
                     RNIKELNNLKDFSINGNEVTFATIGNASTAEGHFWEAVNAIGVLKAPAVITVYDDGYG
                     ISVPNQFQMVKENIGALLKGFERDPRTPHNKVQNGYDHYTVRAWDYSALIETYQTAAE
                     VAREYHIPALVHVVDVTQPQGHSTSGSHERYKTPERLKWEEEFDGLKKMREWMITNRV
                     ISAPELDALEKADYDTVEGFRKAAWEAYLAPLTEERNQVMDILEEIASSTPNHVAELR
                     MLRDRLTALPSFSRRDIHSIAHEALRLLRNESHPSKQVLIQWKHENDKLTEERYSSHL
                     YSGTALKVEEIKPVYSDSSPTMFGFEVINAAFDAALTRDSKLIAFGEDVGKLGDVNQG
                     FKGMQEKYGEFRVTDTGIREATIMGQAIGMAMRGLRPIAEIQYLDYVLYALQTMSDDL
                     ASLHWRTAGGQKAPVIVRTRGHRLEGVWHAGSPMSGILSLTRGMYVLVPRDMTRAAGF
                     YNTLLKSDEPAIVVEVLNGYRVKEKLPDNIGEITLPLGVPETIRQGKDITIVTYGACC
                     RIVLDAAEKLSKVGIDVEVIDVQSLMPFDIHGKIVESLKNTNRILFVDEDVPGGATAY
                     MMQEVIEKQGGYFHLDSPARTLSAKAHRPSYGSDGDYWSKPNAETVFDVVYEMMHEVN
                     PTAFPLFS"
     gene            complement(3414..3782)
                     /locus_tag="PLT08_04965"
     CDS             complement(3414..3782)
                     /locus_tag="PLT08_04965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE03843.1"
                     /translation="MSEAKKVVIERKKGYLSVEYAKPYNIDDLLSFSKEALEVSKAEG
                     YKKLFLDISKMPGRIKPMERYEVGVKAATLFRYKLKVAVLYKTEEINRFAETVALNRG
                     MNVKIFNTKEEAISWLEVEE"
     gene            complement(3815..>5157)
                     /locus_tag="PLT08_04970"
     CDS             complement(3815..>5157)
                     /locus_tag="PLT08_04970"
                     /inference="COORDINATES: protein motif:HMM:NF014774.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HNE03844.1"
                     /translation="DYHRIWINPDNPDIMLIASDQGAIITVNGGKTFSSWYNQPTAQF
                     YHVSTDNAFPYNVYGGQQESGSVCIASRGNDGQITFREWHAVGAEEYGYVAADPLNPD
                     IVYGGKITKYDKSTGQTQDISPEAVWACTACGACTDICPVGNEPMRDILEIRRSLVLM
                     DNEFPTQLQNAYKGMERNVNPWNVSQGDRMKWAEGMSVPTIEQNAEPEILWWVGCAPA
                     TDSRAQKTAQAFAKILNTAGVNYAVLGKNEACTGDSARRAGREDIFFGLASQNVEILN
                     EVAPKRIVTTCPHCLHTIKNEYPAFGGNYQVIHHTQLINELVGAGKISMNLEGDNMKI
                     TFHDPCYLGRHNKVTDAPRDALKSAGVETVEMPRNGAKSFCCGAGGAQMWKEEEQGTA
                     RVNATRFAEAKSTGADTVAVGCPFCLTMMTDASKGDGGTIQVKDVAEIVAERLK"
BASE COUNT         1202 a         1269 c         1247 g         1439 t
ORIGIN      
        1 agttgacatt cttatgaatg agcaagcctt catccgtcca cgaagaattg gtctgtgggt
       61 aggctttcag ccctgccaca attgccatca taaaatacgc cgtgaacgta aggttgactc
      121 cgtcacgttc gaaagcggct ttgttcgcgg cacgatgctt cgccacccgc gacatatctg
      181 cttccatcac ggtcaacaca tgcggcgaag tatgcttaga ctcgaccata tgctgtgcaa
      241 tcgattttcg catggtggta tgtttgatta aggtgccaag tgttccggtg tcaggtgtca
      301 ggtttgaagg tttgaaagtt gaaatgttgg aaggttgacc tgttcctttt gactttccac
      361 tttcgacgaa attcaacaca tcgtttttcg taatgcgtcc gttcagcccc gtgccgttga
      421 cctgagacaa gtccacgccg tgttcagcgg cgatttttgc cacaacagga gagatgaagc
      481 caagatcgcg gttgattgat tgaggttgtg gaatgacagc aggagattgt tgagtaacag
      541 gtgttggcga agaagcaaca ctttctatgg acgcttgtcc actatctaca gtttcccctg
      601 gtttaccaat gatggctaac agttcgccta cctttgcggg cacaccttcc tgtgcaacga
      661 tctgcaagag agttcccgta gcaggggcgg ggatttctgt gtcaactttg tctgtgttta
      721 cttcgagcaa tggttctaat tcattaatag aatcaccaac ttgtttgagc catttggtga
      781 ccgtaacttc gtccacgcct tcgccgagcc ggggtacata aacttttgta gccatgaatt
      841 tctccagttg gtcgtgctat caactaaaaa gtggaaatgc agtcggattc acttcatgca
      901 tcatctcgta caccacatca aacacggtct cagcattggg cttgctccag taatctccgt
      961 ctgagccata tgatggacga tgcgctttgg cagataatgt acgtgcgggc gagtctaaat
     1021 ggaaataacc gccttgcttc tcgatgactt cttgcatcat ataggcagtt gccccaccgg
     1081 gaacatcttc atctacgaag agaatacggt tggtgttctt taacgactct acgatcttgc
     1141 catgaatatc gaagggcatc agcgattgga cgtcgatcac ttccacatcg attcctacct
     1201 tgctcaattt ctcagcagca tccaacacaa tacgacagca cgccccataa gtgacgatgg
     1261 taatgtcttt gccttggcga atggtttcag gaacacccaa gggtaatgta atttcaccaa
     1321 tgttatcggg caatttctct ttgacacgat acccatttag aacttcaaca acaatagccg
     1381 gctcatccga tttcaataag gtgttgtaga aaccagcggc gcgggtcata tcgcgcggaa
     1441 ccaaaacata catgccgcga gtcaaactaa gaatgccaga catgggcgag cctgcatgcc
     1501 aaacaccttc caaacgatgc ccacgtgtgc ggacgataac aggcgctttc tgtccaccag
     1561 cggtgcgcca atgcaatgaa gcaaggtcat cactcatggt ttgcaacgca tacaagacat
     1621 aatcaaggta ttgaatttca gcaatcgggc gtaatccgcg cattgccatg ccaatggctt
     1681 gtcccataat ggtagcttca cgaataccgg tatcggtcac acggaattcg ccgtattttt
     1741 cttgcatccc cttaaaaccc tgattgacat cgccaagctt gccaacatct tcaccgaaag
     1801 caattagttt tgagtcacgg gtaagtgctg catcaaaggc tgcattgatc acttcaaaac
     1861 caaacatggt gggggagctg tctgaataaa ccggcttgat ttcttccact ttgagtgctg
     1921 tgccagagta caaatgcgaa ctatagcgct cttcggtcag cttgtcattt tcatgcttcc
     1981 attgaatgag aacttgtttc gaaggatgag actcatttcg tagaagacgc aatgcctcat
     2041 gcgcaatgga atggatatcg cgcctcgaaa acgacgggag ggctgtcagc cgatctctga
     2101 gcattcgcaa ctctgcaaca tgattcggag tcgagctggc aatttcttcg aggatatcca
     2161 tcacctgatt gcgttcttcg gtcaacggtg cgagataagc ttcccatgca gctttgcgga
     2221 atccttctac ggtgtcgtag tctgcttttt cgagggcatc cagctcgggc gcggaaatga
     2281 cgcggttcgt gatcatccac tcacgcatct ttttcaagcc gtcaaattct tcttcccact
     2341 tgagccgttc aggcgtctta tatctttcat ggctgccaga ggtggagtga ccctgcggct
     2401 gtgtgacatc cacaacgtgc actagtgcag ggatgtgata ttcgcgcgca acctctgcag
     2461 cggtttgata cgtttcaatg agtgcagaat aatcccaagc gcgaacggtg taatggtcat
     2521 agccgttttg cactttgttg tgtggtgtgc gcgggtcacg ttcaaaacct ttcagcaacg
     2581 ccccaatatt ttctttgacc atctgaaatt gatttggcac cgagatgcca tatccgtcat
     2641 catagacggt gataacggct ggcgctttga gaaccccaat cgcgttgacc gcctcccaaa
     2701 aatgaccttc tgctgtagag gcattgccga tggtggcaaa ggtcacttca ttgccgttga
     2761 tcgaaaaatc tttgagattg tttaattctt tgatgttgcg atataagacc gatgcatacg
     2821 ccaacccaac ggctctgggc atttgagctg ctgtgcaaga gacatcggat gaagtgttat
     2881 agagttgggt ttgtgcacgc caagcaccat tggggtgaag gtgacgggtg gcaaagtgag
     2941 cattcatttg cctgccgcca ctggcggggt cgtattgagc atcggcatgt gcgtataact
     3001 gcgcaaagaa ttcttgaatg ctcatgatac cgagcatgaa catccaggtc tggtctcgat
     3061 aatagcctga acgccaatcg cctttttgaa aagcacgagc aatggcgagt tgagcgatct
     3121 ctttgccatc gccaaaaata ccaaattttg ctttgccact tagaacttca cgtctaccaa
     3181 taagggatgc ctgcctgctt tgataggcaa gacggtaatc ttttatgatc tcatcttttt
     3241 caaggggaag tgcaatcgta cccttcttct tgttgggcat gaatctctcc tgttgagttg
     3301 attgttcaag gattataaca aaggaagagg ggaaaggttt ctcgctggat tttgattgtg
     3361 aaaaaaaaga aaaagcggat aaaactatcc gctttttctt ttttttgttt tttctattct
     3421 tcaacttcaa gccatgaaat ggcttcttct tttgtattga agatttttac attcattccg
     3481 cggttaaggg caacggtctc tgcaaagcgg ttgatctctt cggtcttata taaaaccgcc
     3541 actttgagct tatatcgaaa aagcgtagct gcttttactc caacctcata gcgttccata
     3601 ggcttgatcc tgccaggcat tttggaaata tccaaaaata actttttata gccttcagcc
     3661 ttggaaactt ccaatgcttc ttttgaaaaa cttagaaggt catctatatt gtagggcttt
     3721 gcatattcta cagacagata gccttttttt ctttcaatga ctactttttt cgcttctgac
     3781 atacagttga gtcggcgttt tcaaatttat tcttctattt caatcgttcc gctacgatct
     3841 ctgccacatc tttcacttgg atcgtgcccc catctccttt ggatgcatcg gtcatcatcg
     3901 taaggcagaa gggacagccc actgccacgg tatcggctcc ggtggatttg gcttcggcaa
     3961 agcgagtggc attgaccctt gccgtacctt gttcttcttc tttccacatt tgtgcgccgc
     4021 ctgcaccaca gcagaaagat ttagcaccat tgcgtggcat ttccaccgtt tcaacaccag
     4081 cagatttcag cgcatcacgc ggcgcatcag tgaccttgtt gtggcgccct aaatagcatg
     4141 ggtcatggaa ggtgatcttc atgttatcgc cttcaaggtt catggagatt ttgcctgcgc
     4201 ccacgagttc gttgatcaat tgcgtatggt gaatcacctg atagtttcca ccgaaggcgg
     4261 ggtattcgtt cttgatcgtg tgcagacaat gcggacaagt cgtcacaatg cgttttggcg
     4321 caacttcgtt caagatttct acattctgtg aagccaaacc aaagaatata tcttcgcgtc
     4381 cagcacggcg agccgagtca ccggtgcagg cttcgttctt gcctagtacg gcgtagttaa
     4441 cgccggcagt attcaaaatt tttgcaaaag cttgcgccgt tttttgtgca cgcgagtctg
     4501 tagctggggc acaaccgacc caccataaaa tttcaggttc ggcgttttgt tcaatggtag
     4561 gcacactcat gccttctgcc catttcatgc ggtcaccctg cgaaacattc cacgggttga
     4621 cgttgcgttc catgcctttg taagcgtttt gcagttgggt ggggaattcg ttgtccatca
     4681 acaccagcga gcggcggatt tccagaatgt cgcgcatggg ttcgttgccg accgggcaga
     4741 tgtccgtaca cgcgccacag gctgtacacg cccacaccgc ttcggggctg atatcctgcg
     4801 tctggccggt gcttttgtcg tatttcgtga tcttgccgcc gtacacgatg tcgggattca
     4861 gcggatcggc agcgacgtag ccgtattcct cggcgcccac ggcgtgccat tcgcggaacg
     4921 tgatctggcc gtcgttgccg cggctggcga tgcacaccga gccgctttcc tgctgaccgc
     4981 cgtagacgtt gtagggaaaa gcgttgtcgg tactgacgtg gtaaaactgc gccgtcggct
     5041 ggttgtacca cgacgaaaag gtctttccgc cgtttaccgt aataatggcg ccctggtcgc
     5101 tggcgatgag cataatgtcc ggattgtccg ggttgatcca gatgcggtgg tagtcgt
//
LOCUS       DAOQJE010000134         5137 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_9486_length_5137_cov_5.022235, whole genome shotgun sequence.
ACCESSION   DAOQJE010000134 DAOQJE010000000
VERSION     DAOQJE010000134.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 5137)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5137)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5137
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9486_length_5137_cov_5.022235"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..355)
                     /locus_tag="PLT08_04975"
     CDS             complement(<1..355)
                     /locus_tag="PLT08_04975"
                     /inference="COORDINATES: protein motif:HMM:NF013381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HNE03845.1"
                     /translation="MNSLQRIKNVISGKPVDRTPNLDIFMIRAVHHLGIPLSKYYLDY
                     RVLCEVNMAVLKDFELDVVQAISDPYREACDMGLEVEFPYDKMPMHRNLVIHEPEDLA
                     KIKFPVQDFGSRMTDR"
     gene            complement(853..2988)
                     /locus_tag="PLT08_04980"
     CDS             complement(853..2988)
                     /locus_tag="PLT08_04980"
                     /inference="COORDINATES: protein motif:HMM:NF012364.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase domain-containing protein"
                     /protein_id="HNE03846.1"
                     /translation="MDKVNGWLFDLYEHPEYGLVLWVIADSGERLRLRMEFPTVFYVA
                     GDFPLLRQAWVFLRKESTKLERVTRRDLFIGDREVMQVTLHKQSSLIHELQRQFPSLD
                     YYDADIPIALRFIAETDVHLLGRCRVILNGEWVKTIEPLDSPWQLSPNRIPLRIMELA
                     PDADPAFRAPTRLKVRYEAVEYVMNIEPHRALLISLQADLRRFDPDLILTRHGDPWLI
                     PMLIQWSQETGLGLDLNRDKHCNVLQKKANSYFTYGQVVHRRAQSHLFGRWHIDRKNA
                     MLFSEYELEGVFEQARVTGIGVQEMARKSPGAGITAMQMQTALKNKVMVPLHKQQAED
                     RKSLAELIRADKGGLVYQPLIGVHKDVAQIDFSSMYPGIMVHYNVSPETIHKTNPEEG
                     LIPKTLKPLLEKRLAIKKLLTELDLRDCEIPVLKQRSDALKWLLVVCFGYLGYKNARF
                     GKIESHETVTAISRELMLQAKEIAEDMGFDVLHMYVDSLFVRKDGLKNKSDFDTLLKM
                     IAEQTRIPIALEGIYRWVTFLPSRRDKRVPVPNRYFGVFQNGDVKYRGIEARRRDTPY
                     WVRKIQLEVLDILAKAEALEEIPTWLPYTSDHVARAKRDLRNGNIPLEELVITQSLSR
                     AVEEYKVPSPAAQAGMQLQLAGKLVAPGQFMRFVYVYGKERVRAWELGVDPRTVDMKR
                     YCLLLDRAVTTVIEEIKTEPQSFFCNPVL"
     gene            complement(2981..3244)
                     /locus_tag="PLT08_04985"
     CDS             complement(2981..3244)
                     /locus_tag="PLT08_04985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE03847.1"
                     /translation="MGRTVVTITQLLNETEASLSPFRRSLRRSDQYIFDGILASARRH
                     ITAISQTDSLLPFEAALLAMLLEQAKEIAVLRQELDQLMGKDG"
     gene            complement(3251..3784)
                     /locus_tag="PLT08_04990"
     CDS             complement(3251..3784)
                     /locus_tag="PLT08_04990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE03848.1"
                     /translation="MNIIPYLKNNRFVVVLSLHSAKEYIPTLIAELALRGPVTVLDAG
                     NQFQAYRIVQLIRLRSLHVDDIAKRIAVRRAFTCYQTVSLLAETPAQPHPYVILDLLA
                     SFYDDQVSILECRRLLTICIKEIERLSFTAPVVLDLPPIRVEAKSFLMDELCRRADEV
                     FILETLPAIQEKQLALF"
     gene            complement(3908..4219)
                     /locus_tag="PLT08_04995"
     CDS             complement(3908..4219)
                     /locus_tag="PLT08_04995"
                     /inference="COORDINATES: protein motif:HMM:NF012422.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HNE03849.1"
                     /translation="MTTLSQHDGRPEDELIQNLLAAGLTHYYESEELWKKWEILSPRE
                     KEITALVCLGYTNKQAAERLVLSPETVKSHLRNVLIKFNLSTRSELRMALEDWDFSAW
                     T"
BASE COUNT         1356 a         1289 c         1137 g         1355 t
ORIGIN      
        1 ggcggtctgt catgcgcgaa ccaaaatctt gcaccgggaa tttgatcttg gcaagatcct
       61 ccggctcatg gataaccagg ttcctatgca tgggcatttt gtcgtatgga aattcaactt
      121 ccaagcccat gtcgcaggct tcacgatatg ggtctgagat ggcttgcaca acatccagct
      181 caaagtcttt caagacagcc atgttcactt cgcataacac gcggtaatcg agataatatt
      241 tcgataaggg gatgccgagg tgatgcacgg cgcgaatcat gaaaatatcg aggttgggag
      301 tgcggtccac cggcttgccg gagatgacat tttttatgcg ttgcagtgag ttcatgcaat
      361 tacctccata gattggatgt gtttcttgtt tgaaactaca tgatagcaaa taatatagca
      421 attcaacttt attttgaagt accggcgcct tacactcttt gagcaacaat gcaaatgaat
      481 gcatcagaaa accgaacggg atgtgcgtcc gtttgcactt catgtcaact ttgccgcctt
      541 tgccctcaca tttttaatgt gagatttaat tgccccaaag tatcgtctaa ggtatcatgt
      601 agtatcaaga gaacacaaca cacgaaagga aacttgcata aaacagcaat ttcattgacg
      661 aaaaacatgc cgttttttgt gtaaaaaatt atgaaccctt cttctgtcac cgctcaagca
      721 aatccgaaca tcgccttcat caagtagtca cactcgatac actattgaac tgaccgcgag
      781 gttcgttgtt ttacctgtat tgcctactaa aagtccatag acaaatgtaa taagtaagct
      841 ttccctaatg atttataaaa ctgggttaca gaaaaaagac tgtggctcag tttttatttc
      901 ttctatcacc gtggtcactg cccggtccaa taacaaacaa tacctcttca tatccacagt
      961 gcggggatct acccccaact cccaggcacg gaccctctcc ttgccgtata cataaacgaa
     1021 cctcataaat tgtcctgggg caaccagttt ccctgccagt tgcaattgca ttccggcttg
     1081 tgcagcaggc gaagggactt tgtattcttc cacagcgcgg ctcaggcttt gggtgatcac
     1141 caattcttcc aacgggatgt tcccatttcg aaggtcgcgt tttgcacgcg caacatgatc
     1201 acttgtgtat ggcagccagg ttggtatctc ttccagtgcc tccgcctttg ccagaatatc
     1261 cagcacttcc aattgaattt ttcgcaccca atagggtgta tctctgcgcc ttgcttcaat
     1321 gccgcggtat ttcacatctc cattttggaa gacaccaaag taacgatttg gaaccggcac
     1381 ccgcttgtcc ctacgggatg gaaggaaagt cacccaacga taaattccct caagtgcaat
     1441 cgggattcgg gtttgttctg cgatcatttt tagcagggta tcaaaatcgc tcttgttttt
     1501 caaaccatcc ttccgcacaa agaggctatc aacatacata tgaaggacat cgaaccccat
     1561 atcctcggca atctctttgg cttgcagcat caactcgcgg ctgatggctg taacagtttc
     1621 atgagattcg atcttcccga acctggcgtt cttatatccc aaatatccaa agcaaaccac
     1681 cagcagccac ttcaatgcat ccgatctttg cttgaggacg gggatttcgc aatcgcgcag
     1741 atctaattcg gtcaataact ttttgatcgc caatcgcttt tccaacaggg gcttcaaggt
     1801 ttttggaatc aacccctctt ctggatttgt cttgtggatc gtttctggag agacgttgta
     1861 atgcaccatg atgcccgggt acatcgaaga gaaatcaatc tgggctacat ctttatgcac
     1921 cccgatcaaa ggttggtaga ccagcccgcc tttatcggcg cggattaact cagcaaggct
     1981 cttcctatcc tcagcttgtt gtttatgcag aggaaccatt accttgttct ttaacgcggt
     2041 ctgcatctgc attgcagtaa tgccagcccc cggagacttg cgtgccatct cctgaactcc
     2101 aatgccggtc actcgcgcct gttcgaagac tccctccagt tcatattcac taaacaacat
     2161 tgcgttcttg cggtcaatat gccagcgccc aaacaaatga ctctgcgcgc ggcgatgcac
     2221 cacttgccca taggtaaagt agctgttagc tttcttttgc aggacattgc aatgtttgtc
     2281 acggttcaga tccaatccca accccgtctc ttgagaccat tgaatcaaca ttggtatcag
     2341 ccagggatca ccatggcggg tgagaatgag atctggatcg aagcgccgca agtcggcttg
     2401 cagactgatg agcaatgccc gatgcggctc gatattcatc acatattcca ccgcctcata
     2461 gcggaccttc aaccgggtcg gcgcacgaaa ggcagggtcg gcgtctggag ccaattccat
     2521 gatgcgcaat gggatgcgat tgggcgataa ctgccagggc gaatccaaag gctcaatggt
     2581 tttaacccac tccccgttta gaatgacccg gcaacgacca agcaggtgaa catctgtttc
     2641 tgcaatgaac cgcaacgcga tgggaatgtc ggcatcgtaa taatccaagg aaggaaattg
     2701 acgctgcaat tcatggatca gactactttg tttatgaagc gtgacctgca ttacttcacg
     2761 atccccgata aaaagatcgc ggcgtgttac ccgctccaat ttcgtactct ccttccgaag
     2821 gaaaacccac gcctggcgca gcagcgggaa atcgcccgca acataaaaaa cggttggaaa
     2881 ttccatcctc aaccgcaggc gttcaccact gtcggcgatg acccacaaaa ccaaaccgta
     2941 ttcaggatgt tcataaagat cgaagagcca gccgttgacc ttatccatct ttacccatca
     3001 actggtcaag ctcctgccgc aggacagcaa tctcctttgc ctgctcaagc agcatcgcca
     3061 acaatgccgc ctcaaaagga agaagtgaat ccgtctggct aatggcagtg atatgtctcc
     3121 gtgcagaggc gaggatgccg tcaaagatgt actgatcgct ccgccgcagg gagcggcgga
     3181 agggtgaaag cgatgcttcc gtctcattga gcagttgcgt gatcgtaact acggttcgtc
     3241 ccatattcac ctaaaacaaa gccagttgtt tttcttgtat cgcgggaagt gtttccaata
     3301 taaagacttc atctgcccgc ctgcatagtt cgtccatgag aaatgatttt gcctccaccc
     3361 gaatgggagg caggtcgagt acgacaggtg ccgtaaagct tagccgctcg atctctttga
     3421 tacagatagt taacaatctt cggcactcga ggatggagac ctgatcgtcg taaaaagaag
     3481 ccagcaggtc cagtatcacg tacgggtggg gttgagccgg tgtttcggct aacaggctga
     3541 cggtttggta gcaggtgaaa gcccgccgca ctgcaatccg tttggcaatg tcatccacat
     3601 gcagggatcg taggcggatg agttgtacaa tgcgatacgc ctggaattga ttaccggcat
     3661 ccaatacagt gacgggtccg cgcagggcaa gctcggcgat cagggttggg atgtattctt
     3721 tggcgctatg taatgaaaga accactacga atctgttatt ttttaagtaa gggatgatgt
     3781 tcatgccaac acaataacag taggcaagaa tataaaaaat cccctgatgg gggatttgga
     3841 ggttgaatat cgtttaaccg agcgggggat gaatatgtgg gtatagatat ggaacgaacc
     3901 agcacatcta tgtccaagcg ctgaaatccc aatcctcaag cgccatccta agctcggacc
     3961 gagtgctcag gttgaacttg atcaggacat tgcgcaggtg tgattttacg gtttcagggg
     4021 agaggaccaa acgctccgca gcctgtttgt tggtataccc gagacacacc aaagcggtaa
     4081 tctccttctc gcgcggcgaa aggatttccc atttcttcca tagttcttcg gattcatagt
     4141 agtgggtgag gcctgcggcc agcaggttct ggatcaactc gtcttcagga cgcccgtcgt
     4201 gctgggagag ggtggtcaaa gttacttgca tggactcact gacctgatat ttccgagggg
     4261 cggataaggg gcgcaagcct atcacggcca aaagtttgtg ccagatagac atgcgccgat
     4321 tgtaatagaa caagtgttct gttgtcaaat tgaatatgat tttggctgct agaaaatctt
     4381 gaatattttt tctcgttatt atcccaattc tcattcaaat caagattcat taatcatcac
     4441 ccaagagaac cttactatct ttaattccaa ctagcaatga tcaattgttt ggtaataaaa
     4501 atagtatcat caccacatga ttaaatattc ttctcaagcg tcttctaaca ttgtatccat
     4561 caagtagtca cattggatat actattgaac taactgcaag gtttgttgtt ttacctggat
     4621 tgcctaataa aaactcgtag atatattttg agtaagtctt tctctaagag atataaaagc
     4681 tgggttatag aaacaaactg tgactcagtc tttatttctc ccttcactgt attcactgcc
     4741 cgatccaata acaaacaata tctcttcaaa tccacagtac gagaatccac tcccaatccc
     4801 caagcacgaa cctattccat tcctaacaca taaacgaacc tcataaactc cccaggagca
     4861 accgtcttcc ctgtcagttg caattgcgtc ccagcctcta cagcaaggga agggtctccc
     4921 gtttcttcta tatgctctag aacatcactt ctctcctgtg cgggtcgtaa cgccagcaga
     4981 atatgttctc tcgatttttg gacatagcaa tattcatgcc ttggggaaac tctcccaact
     5041 tcgaagagca atcccatttc cacctaaaac atcgacaaat ttcataaatt gtccgacaaa
     5101 ggtacttttg gtctcttcag ggggccattt gtccctc
//
LOCUS       DAOQJE010000135         5134 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_9495_length_5134_cov_5.186848, whole genome shotgun sequence.
ACCESSION   DAOQJE010000135 DAOQJE010000000
VERSION     DAOQJE010000135.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 5134)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5134)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5134
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9495_length_5134_cov_5.186848"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..706
                     /locus_tag="PLT08_05000"
     CDS             <1..706
                     /locus_tag="PLT08_05000"
                     /inference="COORDINATES: protein motif:HMM:NF024989.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="NAD(P)-dependent alcohol dehydrogenase"
                     /protein_id="HNE03850.1"
                     /translation="GLGLLGTYAEYICVPEDGALVLKPANITYEEAAALPNGALTALP
                     FLRDKGNIQSGQRVLINGASGAVGTIAVQLAKYFGADVTGVCSTTNLEMVRSLGADKV
                     IDYTKEDFTKNGQTYDIIFDTVGKSPFSSCKGSLTDNGIYMTTVPSPATMLQMLWTSR
                     TGGKKVAIAATGLRPSNEKIKELVFLNELIETGKIKAVIDRIYPLEQIAEAHTYVEAG
                     HKKGNVVIAIGYDDRI"
     gene            731..1435
                     /locus_tag="PLT08_05005"
     CDS             731..1435
                     /locus_tag="PLT08_05005"
                     /inference="COORDINATES: protein motif:HMM:NF025687.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4386 domain-containing protein"
                     /protein_id="HNE03851.1"
                     /translation="MNSAKRENNVTTKVNTYRTTAKVVGALYILGFVVGIAGSMLGTP
                     GQIETISARSMMIAIGALLWVIAAAGDAAHGVMMFPILKQNNERIALGYLSARLVEAA
                     IIAVSALFILLQIPLGVGFLKASASETSYLQSLSALFVQSQAYTYQIGMVALGMAGLT
                     LCYGFYRAKLVPQFFIIWGFIGYVSFLIGSMLEILGFNLQLLHTLPGGLWEMSVGVWL
                     IVKEFNSAAFVSESVE"
     gene            1457..2164
                     /locus_tag="PLT08_05010"
     CDS             1457..2164
                     /locus_tag="PLT08_05010"
                     /inference="COORDINATES: protein motif:HMM:NF012792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNE03852.1"
                     /translation="MSFATFWIEDTLTPIASLPGFTAGPAFDWHELAEINHISHAEVM
                     DRRNNGHRPYVARMTGQPVAYGWLATKKISIGELNIHVELPPDDRYLWDFATLPNWQG
                     RGLYPRLLQAILEQEIQNAKRFWIIHAPENLPSGAGMSKAGFEFIGQLSYAADGKVGL
                     APYPDSTERARIGADLLQVPTIESVLAPCWHCGGAIAKHPEIADANLCWPPQTANGSA
                     CCCVKEVKKSNNVITIT"
     gene            2240..3241
                     /locus_tag="PLT08_05015"
     CDS             2240..3241
                     /locus_tag="PLT08_05015"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HNE03853.1"
                     /translation="MNSQKQNGKMNPSKGKGCLVWLGRIAVSIVGLLLVGYIYEPIAE
                     STDAKAYPPPGQLVDVGGYRLHINCTGTGNPTVIIEAGLENWSTLWAAYVQPEVAKTT
                     RVCTYDRAGMGWSEAGSMPRDAAQLSKELHTLLQEADIPGPYVMVGHSLGGLVVRVFV
                     ADYSSEVAGVVLIDSMNPKQLLSQSQEVLSQSESKSQPFSFKAMLARFGIARLLVKLP
                     GIAPSLGPDLEAYYPLYIRPQSFQTTANESQGMPAAGAQASAVKTFGDLPLIVLTAKL
                     NDLPGWMEWQSELLQLSSNSQQMFAENSGHNIQVDEPQAAVAAIIQMVQQVREMGTK"
     gene            3247..4737
                     /locus_tag="PLT08_05020"
     CDS             3247..4737
                     /locus_tag="PLT08_05020"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HNE03854.1"
                     /translation="MNRKITYISFTILLLISSVLGVSRSFAKAERTEFEEVDAYIIAK
                     MNELGIPGAALVIVEGDQVIHLKVFGIADADGRPVTPQTPFFTGSTGKSFTALAIMQL
                     VEAGKVKLDAPVRTYLPWFRVADVEASKLITIRHLLNQTSGLPVSIGREQLANTDLSE
                     SAIENNVRALAKIELNALPGERYEYSNANYVTLGMVIQAVAGQSYEAYIRQHIFKPLD
                     MQNSFTSKTEAQQDGLALGYQKWFGIPIASPNLPFSRGSLPAGQLILSAEDFGHYLIA
                     QLNDGNYQEVSILSPAGIAALHNPDVPMTGSMDFYGMGWEVQHFQDVEVIRHNGEVPG
                     YTADMFLVPEKNIAVAMVMNTYSPMLGIRASRLPSSVLRILLGQEIIPGNEFLYMRII
                     YGLVMLIPVLQIIAVLTTLRRFLSLQKSAQSSMPLQIALPLIWDAVIAYVLLVTLPGN
                     FGADIPTMILFQPDVGWTAVLSGVFAIVWGFLRTSLFLRSQRDAML"
     gene            4865..>5134
                     /locus_tag="PLT08_05025"
     CDS             4865..>5134
                     /locus_tag="PLT08_05025"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNE03855.1"
                     /translation="MQLTINKLGKEYKRGFWGLKDFSLEIKPGILGLLGPNGASKSTF
                     MRMLATITKPTDGTITWNDTDIVKSPDTLRDVLGYLPQDFGVYPNL"
BASE COUNT         1290 a         1233 c         1311 g         1300 t
ORIGIN      
        1 cgggctgggc ttattaggca catatgccga atacatctgc gtgcctgaag atggggcgct
       61 tgtccttaaa ccagccaaca tcacctatga ggaagccgcc gccctgccga acggggcatt
      121 aaccgccctg cctttcctta gggataaggg aaatattcaa agcggacaaa gagttcttat
      181 caatggggct tctggtgcgg taggcaccat tgccgtacaa cttgccaaat acttcggggc
      241 ggatgtcacc ggggtatgca gcaccacgaa tctggaaatg gtgagatcgt tgggtgccga
      301 caaggtcatt gattacacca aagaggactt tacaaagaac ggtcagacgt acgacatcat
      361 ttttgacacg gtcggtaaga gtccgttttc aagttgtaaa ggctcgctca ctgataatgg
      421 gatctatatg acaactgtcc cttccccggc aaccatgctt caaatgttat ggacctccag
      481 gactggtggt aagaaggtcg cgatcgcagc cacaggattg agaccatcca acgaaaagat
      541 caaggaatta gtcttcctta atgagttgat cgagaccggg aagataaaag cggtaattga
      601 tcgaatctat ccgctggagc agatcgctga ggctcacacg tatgtcgagg caggacataa
      661 aaaggggaat gtcgtcattg ccataggtta tgacgacaga atctagaatg cagaaatgag
      721 gagataaaaa atgaattcag ccaaaagaga aaataacgtg actacaaaag tcaacacata
      781 cagaacgaca gccaaagtgg tgggcgcgct atatattttg ggatttgttg tgggaattgc
      841 gggatccatg cttggtacac ccggtcagat tgagacgatt tctgccagaa gcatgatgat
      901 cgcaatcggc gcgttgctct gggtaatcgc cgctgctggc gacgccgccc acggggtgat
      961 gatgtttccg attttgaagc aaaataacga gcggattgca ctcggttacc tcagcgccag
     1021 gctcgtcgaa gccgctatta tcgccgtctc ggcgttgttc atcctgcttc aaattccgct
     1081 gggtgtcggg ttcctgaaag ccagcgcctc cgaaacttcc tatcttcaat ccctgagcgc
     1141 tctgttcgta caatcacagg cttataccta tcagatcggc atggtcgctt taggcatggc
     1201 aggtttaacg ctttgttacg gtttctatcg agcaaaattg gttccgcagt ttttcattat
     1261 ttggggcttc attggatacg taagcttttt gatcggatcc atgttggaaa tcctgggctt
     1321 taatctgcaa ctactccaca cgcttccagg tggattatgg gagatgtccg tcggtgtctg
     1381 gctgatcgtc aaggaattca attctgccgc attcgtttct gaatctgtcg agtaaaaatc
     1441 ctacgaggaa aacaaaatgt cattcgccac cttttggatt gaggataccc tgacaccgat
     1501 cgcctcatta ccggggttta cggctggtcc tgccttcgat tggcatgaac tcgctgagat
     1561 caatcacatc tcccacgcag aagtgatgga ccgtcgaaat aatggtcatc gtccctatgt
     1621 ggcgcgaatg acaggtcaac cggttgcgta tggctggctt gctacaaaaa agatttccat
     1681 cggcgaactc aatatccatg ttgaacttcc gccagacgat cgctatcttt gggattttgc
     1741 aactctccca aattggcagg gtcgtgggtt gtatccgcga ttactgcaag ccatccttga
     1801 gcaggagatc caaaacgcga aacgattttg gatcatccat gcacccgaga atctcccttc
     1861 cggcgcgggc atgagcaaag caggttttga atttataggg caactctctt acgcagcgga
     1921 tggaaaagta ggactcgcac cctacccgga ttcgaccgaa cgtgcccgca tcggcgcgga
     1981 cctgttacag gttcccacca tcgaatcggt tctcgctcca tgctggcact gcggaggggc
     2041 gatcgcaaaa catcctgaaa tagcagatgc gaacttatgc tggccgcctc aaacggccaa
     2101 tggaagcgca tgttgctgcg tgaaggaagt caagaaatca aacaatgtga tcacgatcac
     2161 ttagaaccat gctcctcttt atggtttgta cagcgagcct gaaacgaata tttattggta
     2221 attttatgga gatcgtacaa tgaacagtca gaagcagaac ggaaagatga atccatccaa
     2281 agggaaaggt tgtttagttt ggctgggacg aattgcagtt tcgatcgtag gcttgctgtt
     2341 ggtgggttac atttatgaac ccattgcaga atccacggat gcaaaggcat atccgccgcc
     2401 cggtcaactg gtggatgttg gcgggtatcg tcttcatatt aactgtaccg gcaccggtaa
     2461 ccccactgtg ataattgagg cgggattgga gaattggtca acattgtggg cagcatatgt
     2521 acaaccagaa gtggcaaaga cgactcgggt ctgcacttat gatcgcgctg gcatgggctg
     2581 gagcgaagca ggctcgatgc cgcgcgatgc tgcgcaactc tcaaaggaac tacatacctt
     2641 gctgcaggaa gccgatattc cgggaccgta tgtaatggtc gggcattcgc tcggtggact
     2701 tgtcgtgcgc gtatttgttg ccgattattc ttcagaagtg gcaggggttg tattaataga
     2761 ttcgatgaat ccgaaacaac tattatccca atcacaagag gtactgtctc agtcggaatc
     2821 aaagtctcag ccattttcct ttaaggctat gttggcgcgt tttggaatcg ctcgtttgct
     2881 tgtgaaattg cctggaattg ctcctagttt aggacccgat cttgaagcct actatccgct
     2941 ttatattcgc ccacagtctt tccaaactac tgctaacgaa tctcaaggga tgccagccgc
     3001 tggagcgcag gcttctgccg tgaaaacctt cggtgacctg ccgttgatcg ttctgaccgc
     3061 caaactgaat gacttgccag gttggatgga atggcagtct gaactcctcc agttgtcatc
     3121 caacagccag caaatgtttg cagaaaacag cggtcacaat attcaagtgg atgagccgca
     3181 ggctgctgtt gcagcaatca tccaaatggt tcagcaagtc cgcgagatgg gaacgaagta
     3241 ggctttatga atcgcaaaat aacatatata agttttacga tattgttgct tatttcttcc
     3301 gttcttggcg tcagccgatc cttcgcgaaa gctgagcgta cagaatttga agaagtcgat
     3361 gcatatatca tcgcaaaaat gaatgaactt ggcattcccg gcgcagcgct tgttattgtg
     3421 gagggcgacc aagttattca cctgaaagtg tttgggatcg ccgatgccga tggacgcccg
     3481 gtcacaccgc agacgccatt ctttaccggc tccacgggca agtcgtttac cgcgcttgcc
     3541 atcatgcaat tggtggaagc aggtaaagtt aagttggatg cgcctgttcg aacctacctg
     3601 ccctggtttc gggttgcaga tgtcgaggca tccaaattaa taaccatccg ccatcttctc
     3661 aaccagacca gcggactccc cgtatccatc gggcgggagc agttggcaaa caccgacctg
     3721 agtgaatctg cgatcgaaaa caatgtgagg gcattggcaa agatcgaact gaatgcgctg
     3781 ccgggcgagc ggtatgaata ttccaacgcg aactatgtca ctttgggcat ggtcattcaa
     3841 gcggtcgccg gtcaatccta tgaggcctat atcaggcagc atattttcaa gccccttgat
     3901 atgcagaaca gttttacttc gaaaaccgag gctcaacaag atggactcgc gctgggctat
     3961 cagaaatggt ttgggattcc catcgcctcg cccaacctgc ctttctcacg cggttccctc
     4021 ccggcgggtc aactcatctt gagcgctgag gattttggtc attatttgat cgcacagttg
     4081 aacgatggga actatcagga agtatctatt ctgtcaccgg caggaatcgc tgccttgcat
     4141 aacccggacg ttccgatgac aggttcaatg gatttttacg gcatgggctg ggaagttcag
     4201 cacttccagg atgtggaagt gatcagacac aatggggagg tgccgggata tacagcggat
     4261 atgttccttg tgccggagaa aaacatcgca gttgcgatgg tgatgaacac gtatagtccg
     4321 atgttgggga ttcgtgcatc gcgccttcca agcagcgtac tgcgtatatt acttgggcaa
     4381 gagatcatac cgggaaatga atttctttat atgcggatca tctatggtct cgtgatgcta
     4441 attcccgttc tccaaataat cgccgttctg acaacgcttc gccgtttcct ttctttgcag
     4501 aagagtgcgc aatcctcaat gccattgcaa atcgctttgc cgttgatctg ggacgccgtc
     4561 atcgcgtatg tacttcttgt aaccctgcca ggtaacttcg gggcggacat tccgactatg
     4621 attctcttcc agccggatgt cggctggacg gcggttctta gtggcgtctt cgcgatcgtg
     4681 tggggattct tgcggacaag cctcttttta cgcagtcagc gagatgctat gttgtaataa
     4741 aatagaagac cacctgccct gatggtgcga tcgactgatt aatttggcgg tcaggtgcaa
     4801 ccatttacac tggttctcgt aaacagacta caattctcaa acctttcgct tattggagaa
     4861 ctctatgcaa ctcaccatca acaaattggg aaaagaatac aaacgcggat tttggggact
     4921 caaagatttt tcccttgaaa tcaaacctgg cattttaggg ttactcggtc cgaacggtgc
     4981 gagcaaatcc acgtttatgc ggatgctcgc caccatcacc aaacctacag atggaacgat
     5041 cacatggaat gacacagaca tcgtcaaatc gcctgacacc ctgcgcgatg tgttgggata
     5101 tctaccacaa gactttggcg tttatcccaa cttg
//
LOCUS       DAOQJE010000136         5131 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_9502_length_5131_cov_6.575650, whole genome shotgun sequence.
ACCESSION   DAOQJE010000136 DAOQJE010000000
VERSION     DAOQJE010000136.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 5131)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5131)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5131
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9502_length_5131_cov_6.575650"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            36..671
                     /locus_tag="PLT08_05030"
     CDS             36..671
                     /locus_tag="PLT08_05030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE03856.1"
                     /translation="MKQDSFKSLIAVLTAIVTVLGATAACLASVAVSTAGDNDFAGLD
                     ASIRGQKAEIINYVVAYEHYRAFTSYVRYNELGNLMYDPGADQDTDARNGAFQREVWG
                     IASGISSVFFSPRYITSEGQYDLERELQEEYAKDAQSEDLNPAPYFEESDRQRNISAT
                     LTADMIVFAVSFWFLTLAQATEKKIKYVWAVLGGLMGLAGIIGIFIGRFLQ"
     gene            668..2359
                     /locus_tag="PLT08_05035"
     CDS             668..2359
                     /locus_tag="PLT08_05035"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNE03857.1"
                     /translation="MKTENTSEQRFENMVTIMIASVAIWVAITAYFQNYASNISDQAR
                     RRAQQYAIEATKTEVNGTIQFSYDWQGAFQTWRELSWQITAAQQNGDTKAVERYQELQ
                     ARIAPLSKMLAPQYFDPTVGWPESYKYEAESYLVESTRLSETYLAESDLGNFTDNVAD
                     SLIVQITLLTVSLSLYGLSMALKGRVRWLFIIVGSGIVGVCVLWLGWSLLELLIRPEV
                     NQAAITAYSEGVGLAYQGRYQESIDKFTQAINENQYYAKAYYERGLSYYELGDLQTAI
                     SEMEEARTQGMQEDVSLNWNLGWTYYLAGQYDKAIEINEPILSDNPEVLGMRMNQAIS
                     YLSKGDFENSQKQYDLLLQEAQRQVNEARSQGAEPSASLWFYMDAGALDLQNLIDQLD
                     NNPKDWTQAPEANLITGNHSAIRDFATSQMKRLKESTVALEYTGQLPNTQSEIQVEPF
                     VFGQITGTDAQGFITGFEPASNNIIPYGEDSFTLEFTYSGTPPKQMLWKVYVNGYEDQ
                     SLRIVSTDDISDGATWYKTFGYSYTNIFILSPGEYTVELYADNILVQRGTFYVQE"
     gene            2414..3154
                     /locus_tag="PLT08_05040"
     CDS             2414..3154
                     /locus_tag="PLT08_05040"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF024811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HNE03858.1"
                     /translation="MPTPIRYLIFDLGGTLMHSRADWGPIHEQADEALSQSLRENGIE
                     LSAEVFRSRLHDYYQQRDKDFYETTYHLVLRELLKELGHAEVAESVLRSALDALYVIT
                     QSNWELESDAMETLETLKSQNYKLGIFSNAGDDKDVQELVESFGIRSYFDFVLTSAAC
                     FYRKPHPRAFEIALAQWNATPAEAAMIGDSLEADIIGANELNITSIWLTRRAQFKTED
                     MHRIKPNFSLRKLSELPPTLDLLSFSRR"
     gene            3201..3602
                     /locus_tag="PLT08_05045"
     CDS             3201..3602
                     /locus_tag="PLT08_05045"
                     /inference="COORDINATES: protein motif:HMM:NF013868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HNE03859.1"
                     /translation="MLNFKAETQNARVPVSVLRITGDIDSSNFQTFQTQVDDLVKNGA
                     RHILLDFSNVKRISSAGLRVIHNLFNKLRDIHKDVNDDELRKQMSSGAYKSPYLKLFN
                     LAPQIAEIFSLSGFDIYIQTFDDETEAVQSF"
     gene            3632..5038
                     /locus_tag="PLT08_05050"
     CDS             3632..5038
                     /locus_tag="PLT08_05050"
                     /inference="COORDINATES: protein motif:HMM:NF009881.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNE03860.1"
                     /translation="MPDLFDHAMQERMKSEAPLAARMRPRTLEEYIGQEHIIGEGKLL
                     RRAIEADRLFSSIILWGPPGTGKTTLAQVIANTTRSHFVTISAILAGKADLRVVIDEA
                     LERRRLHNERTILFVDEVHRWNKAQQDALLPHVENGTFTLIGATTENPYFEVIKALIS
                     RSRVFQLRNLNQAETGTLLDRALADKERGYGNKNIQFDEDARNHLIDVASGDARNALN
                     ALELAVESTSPDKDNIIHITLDVAQESIQRRAVLYDKDGDAHYDTISAFIKSVRGSDP
                     DAALYWLAKMIYAGEDPKFIIRRLIILAGEDIGLADPIGLVVASSAAQAFDYIGLPEG
                     IYPIVEATLYLSTAPKSNSAGAYFNAMKKIEEEGQVSVPRHLMDGNRDAAAMGHGKDY
                     VYPHDMDGHFTPQQYLPKRLLGTYFYSPSEEGYETQVKSRLEMWREAQRKALGITEVK
                     TMPAMNEEQIQEMKRKIK"
BASE COUNT         1448 a         1166 c         1153 g         1364 t
ORIGIN      
        1 cataatcgtc ttatcagcca aaggagagat gagttatgaa acaagatagc tttaaatcat
       61 tgatcgctgt tcttaccgcc atcgtaaccg tgctaggagc gacagcggct tgtctggcat
      121 cggttgcagt atctactgct ggtgataatg actttgccgg cttggatgcg tcgattcgtg
      181 gtcagaaggc agagatcatc aactatgttg tcgcgtatga gcattatcgt gcctttacca
      241 gttatgtgcg ctataacgag ttgggcaact tgatgtatga ccctggagca gaccaagata
      301 cagatgcgcg taatggcgct tttcaacgtg aagtttgggg tattgccagt ggtattagtt
      361 ctgttttctt tagcccgcgt tatattactt cagaaggtca gtatgacctt gaacgcgagt
      421 tgcaagaaga atatgcaaaa gatgctcaaa gcgaagattt gaatcccgct ccctatttcg
      481 aagagtctga cagacaacgg aatatctctg caactctcac tgccgatatg atcgtgtttg
      541 ctgtttcatt ttggtttcta actcttgctc aagccaccga aaagaaaatt aagtatgttt
      601 gggctgtgct gggtggtctg atgggcttgg caggtattat tggaattttt attgggaggt
      661 tcttacaatg aaaaccgaaa atacctctga acaacgcttt gaaaatatgg tcaccatcat
      721 gatcgcatcg gttgcgatct gggtggcgat cactgcttat ttccaaaatt acgcctccaa
      781 tatttcagat caagcacgcc gccgtgccca acaatatgcc attgaagcta cgaagaccga
      841 ggtaaacggg accattcaat ttagctatga ttggcagggc gcttttcaaa cctggcgtga
      901 actaagctgg cagatcacag ctgcgcaaca aaatggagat acaaaggcgg tggaacgcta
      961 tcaagagttg caagcccgta ttgctccgct tagcaaaatg cttgcgcctc aatactttga
     1021 cccgactgtt gggtggcccg aatcatacaa atatgaagct gaatcctatt tggtcgagtc
     1081 gacacgcctc tcagagacgt atttggcaga gtctgattta gggaacttta cagacaacgt
     1141 tgccgattca ttgatcgtgc aaattacttt gcttacggtt tctctatcgc tttatggtct
     1201 ttccatggca ttaaagggac gcgtccgctg gttgtttatt atcgtaggct caggcattgt
     1261 tggggtttgt gtcctctggc tgggatggtc tttgcttgag ttgttaattc gccctgaagt
     1321 caaccaagcg gctattacag catactctga aggtgttggg ctcgcatatc aaggacgata
     1381 ccaagaatca attgataaat tcacgcaagc tatcaacgaa aatcagtatt atgcaaaagc
     1441 ctattacgaa cgcggacttt cttattatga attaggagac ctgcaaactg ccatttctga
     1501 aatggaagag gctcgtactc aaggtatgca agaagatgtg agcctgaatt ggaatctagg
     1561 ttggacgtat tacctcgccg ggcagtacga taaagcgatt gaaattaacg agcccatctt
     1621 aagtgataac cctgaagtgc ttggaatgcg gatgaatcaa gccatcagtt atctttccaa
     1681 aggggatttc gagaattcgc aaaaacaata tgaccttctg ctccaagaag cacaaaggca
     1741 agtgaatgag gcacgctcac aaggtgcaga accttccgct tccttatggt tttacatgga
     1801 tgccggcgct ttggatcttc aaaacctaat tgaccagtta gataacaatc ccaaagattg
     1861 gacccaagcc ccagaagcaa atttgatcac gggcaatcat tctgccattc gtgactttgc
     1921 tacttcacaa atgaaacgtt tgaaagaatc tactgtagca ttggaatata cgggtcaact
     1981 gccgaatact caaagtgaaa ttcaagttga gccgtttgtc tttgggcaaa tcaccggaac
     2041 cgatgcgcaa ggttttatca ctggcttcga gccggcaagt aataacatca ttccgtatgg
     2101 cgaagactct ttcacactcg agttcaccta ttcaggaact cctccaaagc aaatgctgtg
     2161 gaaggtatac gtcaacgggt atgaagacca atccttgagg atcgtctcta ccgatgatat
     2221 tagcgatgga gccacttggt acaaaacttt cgggtatagt tatacaaata ttttcatttt
     2281 gagtccgggt gaatacaccg tggaacttta tgcagataac attcttgtac aacgaggaac
     2341 cttctacgtg caagaatagt tatcatttat aagacaggga gtcgttttct cgaacggctc
     2401 cccactaaaa aacatgccta cacctattcg atatttgatc tttgacctcg gcggaaccct
     2461 aatgcactcg cgtgcagatt ggggtcctat tcacgaacag gcagatgaag cgctgtcaca
     2521 gtctttgcgc gaaaacggta tagaactcag cgccgaggtt ttccgctcca gattacacga
     2581 ttattatcaa caacgcgata aagattttta cgaaacaacc tatcatcttg tcttgcgaga
     2641 attgctcaaa gagttgggtc atgctgaggt cgcagaatcc gttttacggt ccgcgctgga
     2701 tgcgttgtac gttatcaccc aaagcaattg ggaattagag tctgatgcca tggagacact
     2761 cgaaaccctc aagtcgcaaa attacaagct aggcatcttt tccaatgctg gcgatgacaa
     2821 agatgttcaa gaacttgtag agtcctttgg tattcgcagt tattttgatt ttgtgcttac
     2881 ctctgcggca tgtttttatc gcaaaccaca tccgcgtgcg tttgagatcg ctcttgcgca
     2941 atggaacgcc accccagccg aagcggcaat gatcggtgat agtctcgaag cggatattat
     3001 tggcgcaaat gaattgaaca taacttccat ttggttgaca cgccgtgcac aattcaaaac
     3061 tgaggatatg catcgcatca aacctaactt tagcctgcgc aaactctcag agctgccacc
     3121 cacgcttgac ctactctctt tctcccgccg ttagaccaaa gcacttcatc taaaatcaaa
     3181 actcatcaaa ggaaatttta atgttgaatt tcaaagctga aacacaaaat gcgcgcgtac
     3241 cagtgagtgt cctgagaatc acaggggata ttgactcgtc gaatttccaa acgtttcaaa
     3301 cacaagtaga tgatctggtt aagaatggcg ctcggcacat tctcctagac tttagcaacg
     3361 tcaagcgcat tagcagtgct gggctgcgcg ttattcacaa tctgttcaat aaattgcgtg
     3421 atattcataa agatgtaaac gatgatgaat tgcgaaaaca aatgagttct ggggcatata
     3481 aatcacctta tttgaaactt ttcaacttgg caccgcaaat tgcagaaatt ttctcactta
     3541 gtgggtttga tatttatatt caaacattcg atgatgagac agaagccgtt caatctttct
     3601 agacaattta cttcacgcta taatcctctc catgccagat ctctttgacc acgccatgca
     3661 agaacggatg aaatctgaag cgccacttgc cgcgcgcatg cgtccacgca cattagaaga
     3721 atatattggt caggagcata ttattggtga agggaaactt ttacgtcgcg ccattgaagc
     3781 ggatcgtttg ttttcctcta tcatcttatg gggacctccc ggcacaggca aaactacact
     3841 tgcacaagtg attgcaaata ccacacgcag tcactttgtg accatctctg ctattttggc
     3901 aggcaaagca gacttgcgtg tggtaatcga tgaagcactg gaacgtcgcc gtttgcacaa
     3961 tgaacgcacc attcttttcg ttgatgaagt tcatcgctgg aataaagccc aacaagatgc
     4021 ccttctccct catgtggaaa atggcacttt cacacttatt ggtgcaacga ctgaaaaccc
     4081 ctactttgaa gttattaaag cgttaatttc gcgttcgcgt gtctttcaac tacgcaacct
     4141 aaaccaagct gaaactggca ctctgttaga ccgcgcgctg gcagacaaag aacgtggtta
     4201 cgggaacaaa aacatccagt ttgatgaaga tgctcgaaat catcttattg acgtagcttc
     4261 tggggatgcc cgaaatgcgc tcaatgcatt agaactggca gttgaatcca caagccctga
     4321 caaggacaat ataatccata tcacccttga tgtcgcacaa gagtctattc aacgacgggc
     4381 ggtgctctac gataaagacg gcgacgcaca ttacgacaca atttcggctt ttatcaaatc
     4441 ggtccgcggc tcagaccccg acgcagcctt atactggctc gcaaaaatga tctacgctgg
     4501 agaagacccc aaatttatca ttcgccgtct catcattctt gctggcgaag atattggctt
     4561 ggctgacccg ataggtttgg ttgtagcctc ttctgccgca caagcatttg actatattgg
     4621 gctccccgaa gggatctacc caattgtcga agcgacgctc tatctttcca ctgcccctaa
     4681 atcaaatagt gctggtgcct atttcaatgc catgaaaaag atcgaagagg aagggcaggt
     4741 ttcggttcca cgtcacctca tggatggaaa tcgtgatgct gcagctatgg gtcatggtaa
     4801 agattacgtc tacccgcatg atatggacgg tcatttcacg ccgcaacaat atcttcccaa
     4861 acgcctactc ggaacctact tctattctcc atcggaagaa ggctatgaga cgcaggtcaa
     4921 atcacggttg gaaatgtggc gcgaagccca acgtaaagca cttggaatta cagaagtcaa
     4981 gactatgcca gccatgaacg aagaacagat ccaagagatg aagaggaaga tcaaataatg
     5041 accttattat tactaatccg tcacggtgag aatgagtatg tcaaaacagg gaaaatggca
     5101 gggcgaattc ccggcgtgca cctaaatgaa a
//
LOCUS       DAOQJE010000137         5110 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_9564_length_5110_cov_5.419189, whole genome shotgun sequence.
ACCESSION   DAOQJE010000137 DAOQJE010000000
VERSION     DAOQJE010000137.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 5110)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5110)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5110
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9564_length_5110_cov_5.419189"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>5110)
                     /locus_tag="PLT08_05055"
     CDS             complement(<1..>5110)
                     /locus_tag="PLT08_05055"
                     /inference="COORDINATES: protein motif:HMM:NF024583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HNE03861.1"
                     /translation="ALLMLRLVARTHSDPQESSTSPILTKSNSRDAVIVIQPGGRVEY
                     LSAAARPYFNLREDEPFDLERLARHVRPSDDFIDLCVTPGVRRVSINGKPIELSSYEV
                     PGAYPRMLISVRGKDASAASISNNSSESDEILRVATEFGQTIAASLDLDTTVRSILDH
                     VGRLVPSDVLELKLWNEERQILTPFRFQQAASSKVITASLSRFGGLTDQLSARREPII
                     LNDFESIHEVLSGNELNPIHSYMGIPLMAGGELVGTLEAGQLSSGAFGQHDLNLLHLI
                     SGQAAIAIRNARLYEDEQRRRAELTGLANLNQALGAVRDGQDVIAQLVESIAPLFDAE
                     IVGFLLYDENKRTLEGRNPFRGLPAHFVEIYRATILADSPAEAVIARQEPVITTNAMA
                     DESWRALGLADIATAASLRDIALIPLLSSRRMLGYLQVGHHARGVVSFNAEELRLMSI
                     VANQSASIIANILLVQQARTRAQRADALRRIASLAASSATLDEILKYSVLELARLFNA
                     DRGAVFLLDEARGMLILNSPSMYGVPPEIGDNFIQIFLDDPDFRHTVSGGKRPFLSGH
                     LSSERRILPAYRPLATALAMESAMVVPLVVRDRSVGELMLGSKKQDHFNSSDLQAVST
                     AAGQLAAAIESAGLLSQTDNTLRHRVDQLSFITRVNRELISSLDIRHLLGVLREEAVR
                     ISQADCASAVLFELPSSQTEPVIQLAVGCDRVPTVTPPEWTVIKSGEPLVISDYAREA
                     ILPPHENAVSAVILPVMFQTRVLGLVTVHSNRAGFFTAEAVELLQMLTSQAGIAVNNA
                     NRYQIEKQHSDLMRRRAETLVRLTDVSYNLGHDQPLDQALQMIARAIRDATPFRVVLV
                     SIFEAETSQLRRVTSVGLSQEAWNELSSRKQPLTSVQQLMRSEFKISHSYFIPSDQTP
                     IIPPDVHRVIVDASKTTTNNANTWQAEDFLLLPLENSEGQIVGLISVDDPSDGLRPDK
                     ATIEAVEVFAAQAALLISNTLRQTDLRSRIDSLSAAFDRQQKLINMAKDDLPILLRKD
                     LEQTISLHNLDRRAQRVRAGLAITESVSRQLDASSALSALGRETLTQLGMSVALVAEN
                     TPDGPRLLHVMGSLPRATNVEALFGQRNPMRSCLQNGMPILIPNLDEDDEWRDASLLT
                     SLRAKGVICLPIIVEGKPVAAMLAASPEPMPSFTDEDRHVYTQISQQTSVILQNISLL
                     NQTRRRLDEVNLLLEFSRGLSGMDADSVVESLLASARRVIPHAHAGVVLVWNSKTEHL
                     VPRAVSGYADNDSMMKINYRLGEALPGTAFLNRKSRRVDEINFPRDYNLNTENLAHYR
                     QATGGRLPVSCLIVPIVSAEQNLGLLVLDNFNTTAAFRPDDETLLVSLSQQVALSLDN
                     MRLVQTSQERAGQLQALNDASASLTTSLSTDQLIQSLLDQLGPILPFDTATLWLRERD
                     RLTVISTRGFSDVEERQGLSISVSDSALFKELAQTGQPIFVKDVREDPRFPPVDAPRL
                     SWLGIPLIYKNELMGVLAVEKWQANFYTREQMQAGLTFASQAAVSLDNSRLFEDSVRR
                     ATELDQRSQRLTALNRFTSTLTRSLDTDQILTATAEELFQGLGASRVSIVTFERGQNL
                     WKYTLPRIRAKLPRMLPDAPIFSRLRESQGIFNTDDVRSEPDLVSILEMLGDQARALL
                     ILPLVSGGSLNALVFVQLMGETRFGINELEVARTI"
BASE COUNT         1402 a         1301 c         1268 g         1139 t
ORIGIN      
        1 tgatcgtgcg cgcaacttcc aactcattga taccaaaacg ggtttcgccc atgagttgca
       61 caaagaccaa tgcatttagc gaaccgccgc ttaccaaagg caaaatcaac aacgcccgag
      121 cctggtctcc taacatttca agaatggata caagatccgg ctcactgcgg acatcatcag
      181 tattgaagat accttgtgat tcgcgcaagc gactaaagat aggagcatct ggcagcatac
      241 gcggaagttt ggcacgaatt cgcggcaagg tgtatttcca caagttttgt ccgcgttcaa
      301 aggtcacaat agagacgcgg ctggcaccca agccttggaa caactcttcc gcagttgcag
      361 taagaatttg atccgtatca agcgagcgtg tcagtgtgga tgtaaagcga ttcaacgctg
      421 tgagacgttg tgaacgttga tccaattcag tagcacgtct cacgctgtct tcaaagaggc
      481 gcgagttatc aagtgaaacc gcagcctgtg aagcaaaggt caacccggct tgcatttgtt
      541 cgcgggtata gaaattcgcc tgccactttt caacagccag cacacccatc aattcatttt
      601 tataaataag cggaatgccc aaccaagaaa ggcgtggtgc atccacaggt gggaagcgcg
      661 ggtcttcacg cacatctttg acgaagattg gctgaccagt ctgcgctaac tctttgaaca
      721 aggcactgtc actcacagag atggacaagc cttggcgttc ttctacatcc gagaaaccgc
      781 gagtagaaat cacggttagg cggtcgcgtt ctcgaagcca gagcgtcgcc gtatcgaaag
      841 gtaagatcgg tccaagctga tcaagcagag attgaatcaa ctgatccgtg ctcaagctgg
      901 ttgtcaacga ggcagacgca tcgttcaacg cctgtaattg acctgcgcgc tcttgagagg
      961 tttgtaccaa gcgcatattg tcaagcgaga gcgccacttg ttgtgaaagt gagaccaaca
     1021 aggtttcatc atcagggcgg aaggctgcag tggtgttgaa gttatcgagt acgagcagac
     1081 ccagattttg ttctgctgaa acaatcggca cgatcaaaca agatactggt aaacgcccgc
     1141 ccgttgcctg acggtaatga gcaaggttct cagtattgag gttgtaatca cgcgggaagt
     1201 tgatctcatc cacacggcgt gattttcgat tcaagaatgc tgtaccgggc aaggcttcac
     1261 cgagacgata attgatcttc atcatgctat cgttatcggc ataaccagaa accgcacgag
     1321 gtacaaggtg ctctgtcttg ctattccaaa ccaaaacaac accggcatgt gcatgcggga
     1381 tcacgcgacg agcgctcgct aacagagact caaccaccga gtctgcatcc atgccagata
     1441 agccacggct gaattcaagc aacaagttca cttcgtctaa cctgcggcgg gtttggttca
     1501 agagagaaat gttttgcaga atgaccgaag tctgctgaga gatctgggtg tatacgtggc
     1561 ggtcttcatc agtgaacgat ggcattggct ctgggcttgc cgccaacatg gcagccactg
     1621 gcttgccttc cacgatgatg ggcaaacaga tcacgccttt ggcacgcaaa gacgtaagca
     1681 atgaagcatc gcgccattca tcatcctcat caaggttcgg aatcagaatg ggcataccat
     1741 tttgcaaaca agagcgcatc gggttgcgtt gaccgaacaa tgcttccaca ttggtagcac
     1801 gtggcaggct ccccattaca tgcaacaaac gcggaccgtc tggtgtgttt tcagcgacca
     1861 gcgcaacaga catcccaagt tgggtaagag tttcacgccc gagcgcagaa agagccgagc
     1921 ttgcatccaa ttggcggcta actgattcag tgatcgccaa ccccgcgcgg acgcgttgag
     1981 cacgtcgatc caggttatga agcgagatgg tctgctcaag gtctttgcgt agcaaaatgg
     2041 gcagatcatc tttcgccata ttgatcagct tttgctggcg atcaaatgcc gcagaaagcg
     2101 aatcgatgcg ggaacgtaga tccgtctggc gcaatgtatt gctaataagc aatgcggctt
     2161 gtgcagcgaa gacttccacc gcttcaatcg tcgccttatc tgggcgcaac ccatcagaag
     2221 gatcatccac gctgataagc ccaacgatct gcccctctga attttcaagt ggcaacaaaa
     2281 ggaaatcttc agcctgccag gtgttggcgt tgtttgtagt cgttttcgaa gcgtccacaa
     2341 tcacacgatg aacatccggc gggatgatcg gagtttggtc agacggaata aagtaagagt
     2401 ggctaatctt gaactcagag cgcattaatt gctgcacgct ggtaagaggc tgtttgcgag
     2461 aggaaagttc gttccatgct tcttgcgaaa gtcccacaga tgtgacacgg cgcaattgac
     2521 tagtttctgc ttcaaagatg ctgaccaata ccacacggaa cggagtagca tcgcggatgg
     2581 cgcgtgcaat catttgcaat gcctgatcta acggttgatc atgccccaag ttgtagctga
     2641 catccgtcag acgcacgagg gtttctgcac ggcggcgcat taggtcactg tgttgttttt
     2701 cgatctgata tcgattcgca ttgttgacag caatgcccgc ctgcgaagtg agcatttgca
     2761 agagttccac ggcttcagcc gtgaaaaagc ctgcacggtt tgagtgaacg gtgaccaacc
     2821 caagcacgcg agtctggaac atcactggca aaataaccgc agacaccgca ttttcatgcg
     2881 gcggcaaaat ggcttctcgg gcatagtctg aaatcacaag cggctcaccg ctcttgatta
     2941 cagtccattc aggaggagtc actgtcggaa cgcggtcaca acccactgcc aattggatga
     3001 caggctcggt ttgagaactg ggcaattcaa agagaactgc cgaagcgcaa tcggcttggg
     3061 agatacgaac agcttcttct cttaaaacac ctaacaaatg acgaatatcg agcgaagaaa
     3121 tcaattctcg atttacacgt gtaataaagg aaagttggtc tacacggtgg cgaagtgtat
     3181 tatctgtttg agataacaag cccgcagact caatcgcagc cgccagctga cctgccgctg
     3241 ttgaaactgc ttgtaagtcc gatgagttga agtgatcctg tttcttgcta cccagcatca
     3301 actcacccac actgcggtcg cgaaccacta acggaacgac catggcagat tccatggcga
     3361 gtgcggtggc gagtggacga tatgcgggca gaatacggcg ttctgagctg agatgccccg
     3421 aaaggaaggg acgcttaccg ccagaaacgg tgtgacgaaa atctgggtca tccaagaata
     3481 tttggatgaa gttatccccg atctcaggtg gaactccata catggatgga gaattaagaa
     3541 tcaacatacc acgggcttca tccaacaaga aaaccgcacc gcggtctgca ttgaaaaggc
     3601 gcgccaattc cagaacagaa tattttagaa tttcatcaag ggtggcagaa gatgccgcca
     3661 aacttgcaat acggcgcaat gcatcggcac gctgagcacg agtccgcgct tgctgcacga
     3721 gcaaaatatt cgcgatgatc gaagcagact gattggcaac gatgctcatc aagcgcaatt
     3781 cttcagcatt gaaagaaacc acccctcgag cgtggtgacc aacctgaaga tagccgagca
     3841 tgcgcctcga agaaagaagc ggaatgagag caatatcgcg caaacttgcc gcagttgcaa
     3901 tatccgctaa gcctaacgca cgccaacttt catctgccat ggcattggtt gtgatcaccg
     3961 gctcctgacg cgcaattaca gcttcggcag ggctatcggc caaaatcgtg gcacgataaa
     4021 tttctacaaa gtgggcaggc aaaccacgga agggatttcg tccttccaag gtacgtttat
     4081 tttcatcata tagaagaaaa ccaacaatct ccgcatcaaa cagcggagcg atgctttcta
     4141 ccaattgcgc aattacatcc tgcccatcgc ggacagcccc aagcgcttga tttaaattcg
     4201 cgagccctgt caattcggcg cggcgtcttt gttcatcttc atacagcctt gcattacgaa
     4261 tggcaatcgc tgcttgcccc gagatcaaat gcagaagatt caagtcatgc tgaccgaaag
     4321 caccgctact tagctgacca gcttccaatg tacccaccag ctccccacca gccatgagcg
     4381 gaatgcccat atacgaatga atgggattta attcattacc tgataaaact tcgtgaatgg
     4441 attcaaaatc attaagaatg atcggttctc tccgcgccga taactggtcg gtaagaccgc
     4501 caaagcgcga aagcgacgcc gtaatcactt tgctcgaagc cgcttgctgg aagcgaaatg
     4561 gagtcaggat ctggcgttct tcattccaaa gcttcaattc caaaacatct gaaggaacca
     4621 aacgcccaac atggtcaaga atcgagcgga ccgttgtatc caagtctaag ctagcagcaa
     4681 tggtctgacc aaactctgtt gccacacgca agatttcatc cgattcagat gaattatttg
     4741 agatgcttgc agcgctcgca tctttcccac gcacagaaat caacatgcgc ggatatgccc
     4801 ccggcacttc ataagaagaa agctcaatcg gcttcccatt gatagaaacg cgccgtactc
     4861 ctggagtcac acacagatca ataaaatcat ctgaaggacg cacatgtcgt gcaagacgct
     4921 ccaagtcaaa tggttcatct tctcgtaaat taaagtatgg ccgtgcagcc gcgctaagat
     4981 attccacccg tcccccgggt tgaataacaa tcaccgcatc gcgcgaattc gattttgtaa
     5041 ggattgggga agttgaactt tcctgggggt ctgaatgggt acgagcaaca aggcgtagca
     5101 tcaaaagagc
//
LOCUS       DAOQJE010000138         5094 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_9602_length_5094_cov_5.638817, whole genome shotgun sequence.
ACCESSION   DAOQJE010000138 DAOQJE010000000
VERSION     DAOQJE010000138.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 5094)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5094)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5094
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9602_length_5094_cov_5.638817"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..449
                     /locus_tag="PLT08_05060"
     CDS             <1..449
                     /locus_tag="PLT08_05060"
                     /inference="COORDINATES: protein motif:HMM:NF012698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HNE03862.1"
                     /translation="MLTARDAIENRVEGLESGADDYLVKPFAPAELVARVHAMLRRVD
                     AKPENQRASYADVSLDPSTREAKRGDMDLNLTVTEFNLLHLLLRHPRQVLERRQILND
                     VWGYDFGGDDNVLEIYVGYLRKKLEAEGGSRLIQTVRGVGYALREE"
     gene            449..1885
                     /locus_tag="PLT08_05065"
     CDS             449..1885
                     /locus_tag="PLT08_05065"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase dimerization/phospho-acceptor
                     domain-containing protein"
                     /protein_id="HNE03863.1"
                     /translation="MSIRLRFTLLYTFILALTLSIFGISLYAIQAQSTLDSLKRDLVD
                     GASKLSEITLKTNFPNDSTQRPPNEQKPPPVTFDQFSSDQNLKDIREHEILRVLDPNG
                     NLVASPFGREEDALPLSVEGLQAAQNGGDWYESGMVSDEEMLIYSRPVIVEDEVVYIL
                     QVARPLTERNRTLQSLSITLVVAGALTILIAFGVGWVFARFTLRPIQRITHTAQHIGD
                     ERDFSRRVDYVGPQDEVGQLASTFNTMLTRLQDAFQKVEHSLQMQRDFVADVSHELRT
                     PLTTLRGNLGLLRRNPAPEEQTDILNDMVDESDRLIRLVNDLLQLARADAGRSLVKES
                     VEISPLLQEVVRQAQLLDPQRTIQLNAADPLQVRGDRDAIKQVLIIGLDNALKHSSSQ
                     VEVICKKLDGRVEIQIQDHGDGIPPEKLEHVFDRFYRGEDSASVPGFGLGLSIAKSLI
                     ERQDGTIQMNSQVGKGSVLKLRLMISSN"
     gene            complement(1931..2524)
                     /locus_tag="PLT08_05070"
     CDS             complement(1931..2524)
                     /locus_tag="PLT08_05070"
                     /inference="COORDINATES: protein motif:HMM:NF014693.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FmdE family protein"
                     /protein_id="HNE03864.1"
                     /translation="MNLPEILLESSSRHKHLCPRQVLGARMALYAGELLGLELPREDK
                     RLLVTSETDGCAVDGVIAASHCRVGSRTLRILDFGKVAATFTDTHTETSLRIAPQREA
                     RTQADEYASHAKNHWEAMLEAYQIMPFDKLFHVQKVCLITPLSEVISKPGKKILCGIC
                     GEEVINGREILLNESTLCKSCAGESYYLANTPVFADR"
     gene            complement(2521..3579)
                     /locus_tag="PLT08_05075"
     CDS             complement(2521..3579)
                     /locus_tag="PLT08_05075"
                     /inference="COORDINATES: protein motif:HMM:NF021539.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2270 domain-containing protein"
                     /protein_id="HNE03865.1"
                     /translation="MPRKKETPPPPPAQEEESVWTYRGYKLKSSEFVTAMVHLFRAEV
                     QRANVWRQRLDTTTNWAVVATGATLSISFSQPNVHHGVIILNTLLVTWFLFIEARRYR
                     YYELWSYRVRLMETDFYAPMLVPPFHPSPEWAENLAENLLTPSFPISMWEAFGRRLRR
                     NYLWIYLILYISWMGKSFLFPEPATTIAEFVGRGAVGSIPGFVMISLGLAYHLFLLII
                     AIGTVGMTKATGEVLPRFGDETAPAAPSMEGKHTGLRSLLVPRKRRRQLLALIITDKA
                     KEVSSRIMNDLKRGVTSMEGKGMYTGKDRSILMCALTITEAHALKSAVAKEDPKAVVI
                     VSPAQEILGGGFAPLEEK"
     gene            complement(3585..5093)
                     /locus_tag="PLT08_05080"
     CDS             complement(3585..5093)
                     /locus_tag="PLT08_05080"
                     /inference="COORDINATES: protein motif:HMM:NF015557.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HNE03866.1"
                     /translation="MTASAVLSLFMNNIAAASLLFPSLSGVARKMKISPSKLLMPLAF
                     GTILGGMATLLTTVNLVVSGLLRDAKLSAFSLTDFLPVGLPLAFAGIMFMVLWGRKLL
                     PSQSPAQRHEEENASDELIKTYQLAERLVRAEVCKGGSLDGVTLEKSGLREKHHLNVI
                     AVQRGRNTLPIEAKMILKGDDILLIMARPEDSVPESLGEMLEVLPSGEWEQDYLSTPN
                     MKLIEAAIAPRSHLASQTLSEIRFEQKFNSQVLAIWRRGRSIRTRLADLPLEFGDGFL
                     MQGTEKSLSLLRTEPGLVLLAESAPSTRMTWRNWLTVLIMFATLALAVLFSDNIAEIM
                     LTGSVMMVLIGTMSMDQAYRSIDWRSIFLVAGMLPLGVALNKTGASTLFADAILSAFG
                     NASHLVLLASFVLLTVALTQIINGAAAVTVIAPIAIATSQQVGMEPRSVAMAVALASS
                     MAFMSPLGHAVNVMVMGAGGYTFKDYAKVGIPLTILLVIILLIILPILMPIG"
BASE COUNT         1282 a         1392 c         1262 g         1158 t
ORIGIN      
        1 tgatgctcac ggctcgcgac gccatcgaaa accgtgtgga agggctcgaa agcggcgccg
       61 atgattacct ggtcaaacca tttgcacctg ccgaactggt ggcgcgcgtc catgccatgc
      121 tgcgacgggt ggatgccaag ccagagaatc aaagagccag ttatgccgat gtgtctctcg
      181 acccatccac ccgcgaagcc aaacgcggcg atatggactt gaacctcact gtgaccgaat
      241 tcaatttgct gcacctgttg cttcgccatc cgcgccaggt gttggagcgc aggcaaatcc
      301 tcaacgatgt gtggggctac gatttcggcg gcgatgacaa cgtgctcgaa atttacgtcg
      361 gctaccttcg taagaaactc gaagccgaag gcggttcgcg cctcattcaa actgttcgcg
      421 gcgtgggcta cgctctgcgg gaggaataat gtcgattcgt ttgcgcttca ccttgcttta
      481 tacatttatt ctcgcgctga ccttatccat ctttgggatt tctctttatg ccatccaggc
      541 gcaatccaca ctcgattcgc tcaaacgcga cctggtggat ggagccagca aattatctga
      601 gatcacgctg aaaacaaatt tccccaatga cagcacccag cgcccgccaa atgagcaaaa
      661 gccaccgcca gtcacctttg atcaattttc cagcgaccag aacttaaaag acatccgtga
      721 acatgagatc cttcgcgtgc tcgaccccaa cgggaacctg gtcgccagtc catttgggcg
      781 cgaagaggat gccctgccgc tcagcgtaga agggctgcaa gccgcgcaaa acggcggtga
      841 ctggtatgag tcgggcatgg tttcagatga agagatgctc atctacagcc ggcctgtgat
      901 cgtggaagat gaggtggttt acattttgca ggttgcccgt ccgcttacgg agcgcaaccg
      961 caccttgcaa tccctttcga tcaccctggt ggtggctggc gctctcacga tattgatcgc
     1021 ctttggcgtg ggttgggtct tcgcacggtt caccctgcgc cccatccaac gcattacgca
     1081 taccgcacaa catatcggtg atgagcgcga tttctcgcgc cgcgtggatt acgtcggccc
     1141 acaggatgag gtcggccaac ttgcaagcac cttcaacacc atgctcaccc gcttgcaaga
     1201 cgccttccaa aaagtggaac actctctgca aatgcaaagg gattttgttg ctgatgtttc
     1261 gcatgaattg cgcacgccgc tcaccaccct gcgcggaaat ttaggcttgt tacgccgcaa
     1321 ccccgccccc gaagaacaga cggacatcct caacgacatg gtggatgaaa gcgaccgcct
     1381 gattcggctc gtcaatgatc tgctgcaact tgcccgcgcc gatgccggtc gtagtttagt
     1441 caaagagagt gtggaaattt cccccctgct tcaagaggtt gttcggcagg cacagttgct
     1501 cgacccacag cgtacgattc aactcaatgc ggcagatcca cttcaagtca gaggggaccg
     1561 cgatgcgatt aagcaggtgc ttatcatcgg attggataat gcccttaaac attcatcgag
     1621 ccaggtcgaa gtcatctgca aaaaattgga tggacgggtg gagattcaga tccaagatca
     1681 cggcgatgga attccacccg aaaaattgga acatgtcttc gaccgtttct atcgcggcga
     1741 agattctgca tccgtccccg gctttgggct ggggctgtcc attgccaaat ccctaatcga
     1801 gagacaggat ggcactattc aaatgaatag ccaggtaggt aaaggtagtg tgctgaaatt
     1861 aagattaatg atctcaagca attgaactta ttgttcatca ttagggcata ttgccacgtt
     1921 gcactgacct ttatctatct gcaaatactg gggtatttgc cagataataa ctttcgcccg
     1981 cacatgattt acacagagta gattcattaa gcaatatctc gcgcccgttt attacctctt
     2041 ctccacagat gccacagaga atctttttcc ctggcttgct tataacttca gagagcggcg
     2101 taattaaaca gaccttctgc acatgaaata atttatcaaa tggcatgatc tgatatgcct
     2161 ccagcatggc ctcccagtgg ttttttgcgt gagatgcata ttcatccgcc tgagtcctgg
     2221 cttcacgctg tggggcaata cggagcgaag tttcagtgtg ggtatcggta aaggtggcag
     2281 caacttttcc aaagtccagg atgcgaagcg tgcggctgcc aacccggcaa tgactggcgg
     2341 cgatgacccc atccactgcg cagccgtcgg tctcagaagt aacaagcagc cgcttgtctt
     2401 ctcttggcaa ttcaagcccc agtaattccc cggcatacaa tgccatacgc gcgcccagca
     2461 cttggcgcgg acaaaggtgt ttatggcggc ttgagctttc cagcaatatt tccggcaggt
     2521 tcatttttct tccaatgggg caaagccacc gcctaggatc tcctgtgcag gcgaaacgat
     2581 cactactgcc ttaggatctt ccttagcgac cgcggacttg agtgcgtgtg cttctgtgat
     2641 cgttaacgca cacatcaaaa ttgaccggtc tttcccggtg tacatcccct tgccttccat
     2701 ggatgtgact ccacgtttca ggtcattcat gatccgacta gagacttctt ttgctttatc
     2761 cgtaataatg agtgccagca gctgtctgcg tcttttcctg ggaacaagca gcgaacgcaa
     2821 tcccgtatgt ttgccttcca tggatggtgc ggcgggtgcg gtctcatcac cgaagcgcgg
     2881 caggacttca cctgtagcct tggtcatgcc gactgtgcca atggcaatta tcaaaaggaa
     2941 aagatgatat gccagcccaa gcgaaatcat gacaaatcct ggtatggatc ccactgcccc
     3001 gcgcccaaca aattctgcaa tagtggtggc gggctcggga aaaagaaaac ttttccccat
     3061 ccacgaaata taaaggatca aatagatcca aaggtagtta cgccgcaagc ggcgaccgaa
     3121 cgcctcccac atggaaattg gaaaacttgg cgtgagcaaa ttctctgcca gattttccgc
     3181 ccactctggt gagggatgaa acggcggcac aagcatggga gcataaaaat ccgtttccat
     3241 caagcggacg cgataactcc atagttcata ataacgataa cggcgcgctt caataaataa
     3301 aaaccatgta accaataatg tatttaaaat aataacgccg tgatgtacat tgggctgact
     3361 gaacgaaatg gaaagcgttg cacctgtagc aacgaccgcc cagttggtgg ttgtatccaa
     3421 gcgctgacgc cagacattgg cacgctgaac ttctgcacgg aagagatgta ccatggcagt
     3481 gacaaattca ctgctcttga gtttgtaacc gcgatacgtc caaacagatt cttcttcctg
     3541 tgctggtggc ggcgggggag tttctttttt tctgggcatg agatttatcc gatcggcata
     3601 agaatgggga gaatgatcaa aagaataatg acaagcagaa tcgtgagcgg gatgcccacc
     3661 tttgcataat ctttgaaggt ataaccgccc gcgcccatca ccatcacatt gacggcgtgc
     3721 ccaagcggtg acatgaacgc catagacgac gccaacgcga ccgccatggc aacgctgcgc
     3781 ggctccatcc cgacctgctg actggttgcg attgcgatgg gtgcgatcac agtgacagcc
     3841 gccgcgccat tgatgatctg agtcaaagca acggtcagca agacaaaact cgccagcaac
     3901 accagatgac ttgcattccc aaacgcagac aagatggcat ctgcaaacag cgtggacgca
     3961 cccgttttat ttaaggcgac gccaagcggc aacatgcctg caaccaaaaa tatgctgcgc
     4021 cagtcaatgg agcgatacgc ttgatccatg ctcatggtgc cgataagcac catcatcaca
     4081 gaccctgtca gcatgatctc cgcgatgttg tcggagaata ggactgccag cgccaatgtt
     4141 gcaaacatga tcagaaccgt cagccaattg cgccatgtca tgcgcgtgga cggagccgac
     4201 tcagcaagca gaaccagacc tggctccgtt cgcaacaggc ttaaactttt ttcagtgcct
     4261 tgcatcaaaa aaccgtcacc aaattcgagc ggcagatctg ccaagcgtgt gcggatggaa
     4321 cgtccgcgcc gccaaatggc taagacctgc gagttgaact tctgctcaaa gcgaatttca
     4381 gacaaggtct gcgaagcgag atgagagcgc ggcgcgatgg cggcttcgat caatttcata
     4441 ttgggtgtgg aaagataatc ctgctcccat tcgcccgaag gcagcacttc cagcatctcc
     4501 cccagcgatt cgggcacgct atcttcaggg cgagccatga tcagcaaaat gtcatcgcct
     4561 ttcaagatca tcttcgcttc aataggcaaa gtgtttcgtc cgcgttgaac cgcgatcaca
     4621 ttgagatgat gtttttcgcg caagccgctt ttttcgagag tcacaccgtc caaactgccg
     4681 cccttgcaaa cctccgcgcg caccagccgt tcagcaagtt gataggtctt gatcaattca
     4741 tccgaagcat tttcttcttc atgccgttgc gcaggcgatt gacttggtaa taattttcgt
     4801 ccccataaga ccatgaacat gattcccgca aaagccagcg gcaacccaac tggtaaaaaa
     4861 tcggtcaatg aaaaggcaga taatttcgcg tcgcgcaaca gaccactcac cacaaggttg
     4921 accgttgtca gcagcgttgc catgccgccc aaaatggttc cgaacgccaa cggcatcagc
     4981 aatttcgacg gcgatatttt catcttccgc gccacgccag atagcgaagg gaacagcaat
     5041 gaagccgccg cgatgttatt cataaaaagc gacaacaccg cagaagcggt catc
//
LOCUS       DAOQJE010000139         5094 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_9604_length_5094_cov_5.204406, whole genome shotgun sequence.
ACCESSION   DAOQJE010000139 DAOQJE010000000
VERSION     DAOQJE010000139.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 5094)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5094)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5094
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9604_length_5094_cov_5.204406"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            57..296
                     /locus_tag="PLT08_05085"
     CDS             57..296
                     /locus_tag="PLT08_05085"
                     /inference="COORDINATES: protein motif:HMM:NF015956.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046914 - transition metal ion binding
                     [Evidence IEA];
                     GO_process: GO:0006811 - monoatomic ion transport
                     [Evidence IEA];
                     GO_process: GO:0055072 - iron ion homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FeoA family protein"
                     /protein_id="HNE03867.1"
                     /translation="MPDPIQLHLLKPGQSATITRVGGSSALRRRFIEMGIVRGETILI
                     ERNAPLGDPVEYFIKGYHLALRKEEAANIEVVLNG"
     gene            289..792
                     /locus_tag="PLT08_05090"
     CDS             289..792
                     /locus_tag="PLT08_05090"
                     /inference="COORDINATES: protein motif:HMM:NF014474.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FeoB small GTPase domain-containing protein"
                     /protein_id="HNE03868.1"
                     /translation="MADLTIALAGNPNAGKTTIFNALTGLHQHTGNWPGKTVEKKEGE
                     IEHNGKVINIVDLPGTYSLTAYSPEEIIARDYIIEARPDVVINVVDATNLERNLYLTV
                     QILELDVPVVLALNMTDDLQKDGARIDVDQLSQLLGNVPVVQTTANKSKGITELIDKA
                     LRMAKRN"
     gene            797..2479
                     /gene="feoB"
                     /locus_tag="PLT08_05095"
     CDS             797..2479
                     /gene="feoB"
                     /locus_tag="PLT08_05095"
                     /inference="COORDINATES: protein motif:HMM:TIGR00437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015093 - ferrous iron transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein B"
                     /protein_id="HNE03869.1"
                     /translation="MNISQSLFKLDYGGKIEREIAKILTAFEEMKFDTGRYPKRWLAL
                     KLLEADTEILERVQSMPNGEQVIALAKQGADHVQSMLGDDFDLLTADRRYGFINGVVR
                     QSLHRPQMDRITLTDRIDDIVTHKWFGLPVFFAVMYIVFRLVIDVSSPFLDWTDAVIN
                     GPIANGMRFLLNLLPLPAWIHSLVIDGIVAGVGGVLVFVPGLLILYFFLALLEDSGYM
                     SRAAFVMDRFMRIVGLHGKSFIPMILGFGCAVPAIYATRTIASRRDRVLTALLVPLMS
                     CSARLPVYVVFGLAFFGSRAGTVIWAMYALGIVVAMLAGMVFTRTILKPDATSAFLLE
                     LPPYRQPAMKSVLIHMWENTREFVRKAGTMILFTSVVMWFLLNLPWGVTEQRDSYFGK
                     VSNAISPIFTPLGFGSWETGGALVSGFMAKEIVVSTMSQIYLGGEDVEVVEPKTFGEE
                     VIGVGTGFVDATISSGKILLSIIPGVNLIGEEAESEDTALSIALRKQFTPLSAVSLLI
                     FVLLYVPCVATLGAIKHEFGTSWAVTSAVYQTVVAWLMAFIVYQGGLLLGLG"
     gene            2484..2729
                     /locus_tag="PLT08_05100"
     CDS             2484..2729
                     /locus_tag="PLT08_05100"
                     /inference="COORDINATES: protein motif:HMM:NF020581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FeoC-like transcriptional regulator"
                     /protein_id="HNE03870.1"
                     /translation="MLAQLIVLLENKENGLSLSEISRAMNAQPSAVLSMIDLLVKKGK
                     LLEIGPDGKCCSTCGLESECNLLAARGKRYVIAQSLG"
     gene            2907..4334
                     /locus_tag="PLT08_05105"
     CDS             2907..4334
                     /locus_tag="PLT08_05105"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor domain-containing diguanylate cyclase"
                     /protein_id="HNE03871.1"
                     /translation="MFSSDDKEYLVTLFEYAPVALWEQDFSHIKKSFDELRAKGVRSL
                     DAHLVMHPEFLDQCMGKIQVLDVNLQTVAMLKADSKEHLLSNLDKVFRDGMRHHFRDE
                     LRALWNGDVKWSGEGVNYTLEGNPVDIILNWRIMPEYENNWERVLVTTEDITARKRAE
                     QRLHNLFEASPISLWEEDYSAIKKYFDVLRIQGVTSLESYLEEHPEAVSYCVGLINVL
                     DVNQKTLQLFGAESKEHLIANLPRIFRDEMEEHFANELIDLWNGKLSYEREGVNYSLS
                     GEPINILLNFKIMPGYEDDFGWALVSIQDISARKKAEEYLHYLGTHDVMTGLFNRAYF
                     EETIQKLELSRKDPVGVIILDLNYLKKTNDTLGHQAGDKLIRRLAEVMIAAFNNGQIA
                     ARIGGDEFAVILPRTEESEMVDFIKQIHALIEINNKYYREPILSISAGFATSSPGMSL
                     ERVIQLADDAMYRSKAEYHHRRKDD"
     gene            complement(4370..4969)
                     /locus_tag="PLT08_05110"
     CDS             complement(4370..4969)
                     /locus_tag="PLT08_05110"
                     /inference="COORDINATES: protein motif:HMM:NF013933.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysE family transporter"
                     /protein_id="HNE03872.1"
                     /translation="MMLFLRAFIIGISIAAPVGPIGVLCIRRTLANGRLAGFVSGMGA
                     ASADMVYGAIAAFGLTAITGLLVDNAFWFRIVGGLFLLYLGIKTLLEKPSDKPAQAAQ
                     GNLLSMYTSTFLLTITNPMTILSFAAIFAGTMLGKTTGSPLVMVAGVFAGSAAWWLTL
                     SFIVGILRDRLTQVHMAWINRISGAIIIAFGVYALFSGS"
BASE COUNT         1365 a         1031 c         1221 g         1477 t
ORIGIN      
        1 gtttgtcttt ggtagattat gtcattgtga aataaaacta ttttggagta ttccctatgc
       61 ctgaccccat ccaactacat ttattgaaac ccggtcaatc tgccacgatt actcgtgtag
      121 gtggctcgag cgctttacgt cgccgattta ttgaaatggg cattgtccgc ggtgagacga
      181 ttttgatcga gcgaaacgcc ccattaggtg atcccgttga gtatttcatc aaaggttatc
      241 atcttgcctt acggaaggaa gaagctgcca atatagaggt ggtgctcaat ggctgacctg
      301 accattgcgc ttgctgggaa ccccaatgca ggcaagacca ctattttcaa tgcgctgacg
      361 ggcttgcatc aacatacagg caattggccc ggaaaaactg ttgagaaaaa agaaggggaa
      421 attgaacata atggcaaagt catcaatatt gttgacctgc cagggacata cagcctaaca
      481 gcatattcgc ctgaagaaat tattgcccgt gattacatta ttgaagcccg cccagatgtt
      541 gtgattaatg tagttgatgc tactaatctg gagcgtaatc tttacctcac ggttcagatc
      601 ctagaattgg atgtgcctgt tgttctggca cttaatatga ccgatgacct gcaaaaagat
      661 ggcgcacgta ttgatgtcga tcaactctca caactgcttg gaaatgtccc cgtagtgcaa
      721 acaacagcga ataaatccaa gggcatcact gaattgattg ataaagcgct tcgaatggca
      781 aaacggaatt aactttatga acatttctca atctttattt aaacttgatt atggtggcaa
      841 gatcgaacgc gaaattgcga agatacttac ggcttttgaa gagatgaagt tcgataccgg
      901 gcgttacccc aagcgatggc tggcgctcaa gcttcttgaa gccgacactg aaatattgga
      961 acgggttcaa tccatgccca atggggaaca agtgattgca cttgctaaac aaggcgcaga
     1021 ccatgttcag tctatgcttg gtgatgactt tgaccttctc accgcagaca ggcgttatgg
     1081 atttatcaat ggcgttgtgc gccaatcgct tcaccgtccg caaatggata ggatcacact
     1141 tacggacaga attgatgata ttgtcaccca taagtggttt ggtttaccgg ttttctttgc
     1201 tgtaatgtat atcgtctttc gcctcgtgat tgatgtctca tctccattct tggattggac
     1261 ggatgcagta attaacggtc ccattgcgaa tggcatgcgt ttcttgctta acctgcttcc
     1321 gctcccagcg tggattcact cactggtcat tgatggaatc gtcgccggag ttggaggcgt
     1381 gcttgtcttc gtgccggggc tacttatttt gtatttcttc cttgctttgc tcgaagactc
     1441 tggctatatg tctcgcgctg cctttgtcat ggatcgcttc atgcgcattg ttggtttgca
     1501 tggcaaatca tttatcccaa tgatcctggg ctttgggtgt gcggttccgg cgatctatgc
     1561 tactcgtacc attgcaagtc gacgtgaccg tgtgctcact gcattgcttg taccgcttat
     1621 gtcttgctcg gcgcggttgc cggtttatgt ggtctttggg ttggcgtttt tcggttcacg
     1681 cgctggaacc gtgatttggg caatgtatgc gttgggcatt gtggttgcta tgctggctgg
     1741 aatggttttt acacgcacta ttctcaagcc tgatgcaacc tctgcctttt tgttggagtt
     1801 gccgccttat cgtcaacctg ctatgaagag cgtgttgatc cacatgtggg agaatacgcg
     1861 tgaatttgtt cgtaaggcag gcaccatgat tcttttcaca tcggttgtga tgtggttcct
     1921 gctcaacttg ccttggggcg tgactgaaca acgagattct tattttggaa aagtcagcaa
     1981 tgcaatttcg ccgattttta ctccgcttgg ttttggaagt tgggaaacag gtggggcgtt
     2041 ggtttcgggg ttcatggcaa aagaaattgt ggttagcacg atgagccaaa tatatcttgg
     2101 cggcgaagat gttgaagttg tagaacccaa gacatttggc gaagaggtca tcggcgttgg
     2161 cacagggttt gtcgatgcga ccattagctc aggaaagatt ttgttgagca tcatccccgg
     2221 cgtaaatttg attggtgaag aagcggagag cgaagatacg gcgcttagca ttgccttgcg
     2281 taaacaattt actccgctta gcgctgtttc tttactaatt tttgtgttgt tatatgtgcc
     2341 ttgtgttgcc accttgggcg ccatcaagca tgaatttgga acatcatggg cagtcacttc
     2401 ggcggtttat cagaccgtgg tcgcatggtt gatggctttc attgtatatc agggcggttt
     2461 actacttgga ctaggatgat tgaatgcttg ctcaattaat tgtacttctt gaaaataaag
     2521 agaatggttt gagtctttct gagattagtc gtgccatgaa cgctcaacca tccgccgtac
     2581 tttcgatgat cgatttgttg gttaagaaag gtaaactgtt agaaattgga ccagatggga
     2641 agtgctgttc cacttgtgga ttggaatcgg aatgcaactt actcgccgct cgtggaaaac
     2701 gatacgttat agcgcaatca ttaggatgaa gtgcatcgct tcttccttct cctatacctg
     2761 tttcagcttt agaaaaaggt actacgctgt gaccagtttc cggcaaataa caaccggcat
     2821 gaaattgcac ttttttcgaa atgtaatttc atgtgctgct aggtataatc cccttattca
     2881 cttaagaagt agacttggag cggtttatgt ttagctcaga tgataaggag tatttagtga
     2941 ccttgtttga atatgcaccc gttgctcttt gggagcagga ttttagccat atcaagaaat
     3001 catttgatga gttgcgtgct aagggtgtta ggtcattaga tgctcatttg gtgatgcacc
     3061 cagagttttt agaccagtgt atggggaaaa ttcaagtttt ggatgtcaac cttcaaaccg
     3121 ttgccatgct caaagcggat tctaaagaac atttgctttc aaatttagat aaagtttttc
     3181 gtgatggaat gcgccatcat tttcgtgatg aattgcgtgc cttatggaac ggggatgtaa
     3241 aatggtcggg agaaggggtc aactatacat tggagggcaa cccggtagat attattttaa
     3301 actggcgtat catgccagag tacgaaaata attgggaacg ggtgttggtc actacggaag
     3361 atattactgc tcgcaaaaga gctgagcaac ggcttcataa tttgtttgaa gcctctccca
     3421 tttcgttatg ggaggaagat tatagtgcta ttaaaaaata ttttgatgta ttacgaatac
     3481 aaggggttac cagccttgaa agctatcttg aagaacaccc agaagcggtt tcttattgcg
     3541 ttggcttaat aaatgtacta gatgtaaacc aaaaaacttt gcagttgttt ggcgccgaat
     3601 caaaagaaca tctgattgca aatttgcctc gtattttccg tgacgaaatg gaagaacatt
     3661 ttgctaatga gttgatcgac ttatggaacg gcaaactttc ttatgaacgc gaaggggtca
     3721 attattcact aagcggtgaa cccattaata tcctcttgaa tttcaagatc atgccggggt
     3781 atgaagatga ttttggttgg gcattggttt ctattcaaga tatctcagcc cgtaaaaagg
     3841 ctgaggaata tcttcactac ctcggtactc atgacgtaat gaccgggctt ttcaaccgtg
     3901 cttattttga agaaacaatc caaaaattgg aattgagtcg taaagaccca gttggcgtca
     3961 ttattttaga tttgaattat ctaaaaaaga caaacgatac actagggcat caggcaggcg
     4021 ataagttaat tcggcgctta gctgaggtta tgattgcggc atttaataat ggacagattg
     4081 cagctcgtat tggtggcgat gaattcgcag ttattttgcc tcgcacagaa gaaagcgaga
     4141 tggtcgattt catcaaacaa atccatgctc taattgaaat caataataaa tattatcgcg
     4201 aacctattct cagtatttcg gctggttttg caactagttc tccaggcatg agtttagaga
     4261 gagtgataca actagccgat gatgctatgt atagaagcaa agctgaatat caccatagac
     4321 gaaaagatga ctaataaaaa gtgccagcga tatcgctggc actttttatt tagcttccgc
     4381 taaataaggc gtacactcca aaagcaatga tgattgcacc tgaaattcgg ttaatccacg
     4441 ccatatgaac ttgggtcagc cgatctcgta gtattccaac aataaaacta agggttagcc
     4501 accaagcagc ggacccagca aatacgcctg caaccatgac caaaggagac ccggtcgtct
     4561 ttccgagcat ggttccagca aagattgctg caaacgagag aatggtcata gggtttgtga
     4621 tcgtcagcag aaatgtagat gtgtacatac tgagcaggtt gccttgtgcg gcttgagcag
     4681 gcttgtcaga aggcttttcg agcaatgtct ttatgcctag ataaagtaaa aacagcccac
     4741 caacgatacg aaaccagaag gcgttatcta ccagtaatcc cgtaattgca gttaatccaa
     4801 aagcggcaat ggcgccgtaa accatgtctg cactggcagc acccatgcca gaaacaaaac
     4861 ctgccaacct accgttcgct aatgtgcggc ggatgcataa cacgccaatg ggcccgaccg
     4921 gggcagcaat tgaaattccg ataataaaag cacgaagaaa aagcatcatg aatcacctgc
     4981 aagcgacgcg atatgcgtcc aactctaaat ttttatataa agcattgaaa ttcatctggt
     5041 ttgattgggc acaaaactct tgtggagaaa gttcgtactc aatcacgaag actg
//
LOCUS       DAOQJE010000140         5092 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_9608_length_5092_cov_6.467937, whole genome shotgun sequence.
ACCESSION   DAOQJE010000140 DAOQJE010000000
VERSION     DAOQJE010000140.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 5092)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5092)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5092
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9608_length_5092_cov_6.467937"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(80..2152)
                     /gene="fusA"
                     /locus_tag="PLT08_05115"
     CDS             complement(80..2152)
                     /gene="fusA"
                     /locus_tag="PLT08_05115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HNE03873.1"
                     /translation="MARVHPLEKYRNIGIIAHIDAGKTTTTERIMFYTGLTHRIGSVD
                     DGNTVTDWMVQERERGITIVSAAVSAEWKGYQVNIIDTPGHIDFTAEVQRSLRVLDGG
                     VVVFDAVQGVEPQSETVWRQADRYGVPRICFVNKMDRVGASYERTIETIIDRLGAKPI
                     PMQVPIGFEATFKGVVDLLSMTAIVWEDDLGKEPKVTEIPADLKAQAEEARSKMVEKI
                     AELDDELTMKFLEAEEISVDELKAALRKGVIANKATPVFCGSSLKNKGVQAMLDAVVD
                     YLPSPIDKPTISASEPGNPENTFELPTVDDAPLSALVFKIVTDPYVGRLAYIRVYSGV
                     LSQGQTIQNSTKGKKERIGRLLKMHADRREDITEIRAGDIGAVLGFKDSFTGDTLCDN
                     KSLVLESISFPEPVISIAIEPKSTGDQEKMGEALRKLAEEDPTFRVNSDDSTGQTVIR
                     GMGELHLDIIVDRLLREFKVQANVGTPRVAYRESITKPVKEVNYKYAKQSGGKGQYGH
                     VVFALEPGERGSGIIFENKIVGGSIPKEYINPVEKGFREAAESGVLAGYPVVDLKITL
                     FDGSFHEVDSSEMAFKLAASMGFKEGMQKGNPILLEPMMKVEVVAPEEYLGDVMGQVN
                     SRRGLIQGMDVRPGNAQAVRAMVPLGEMFGYATQLRSATQGRGVFSMEFDHYAPVAQS
                     VAEEILKS"
     gene            complement(2187..2654)
                     /gene="rpsG"
                     /locus_tag="PLT08_05120"
     CDS             complement(2187..2654)
                     /gene="rpsG"
                     /locus_tag="PLT08_05120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HNE03874.1"
                     /translation="MRRAKPEKRELLPDVRFNSINIQTMIQHVLKSGKKSVAIGLVYD
                     AMDLIKERTEKNPMEVFDAALKNVGPVMEVRPRRVGGATYQVPMEVSADRRTTLAIRW
                     ILGSARDRSGKSFADKLASELIDASNETGASIRKRDETHKMAEANRAFSHYRI"
     gene            complement(2676..3092)
                     /gene="rpsL"
                     /locus_tag="PLT08_05125"
     CDS             complement(2676..3092)
                     /gene="rpsL"
                     /locus_tag="PLT08_05125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018003268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HNE03875.1"
                     /translation="MPTVNQMVRKGRKDKKSKSKAPALQFTLNSYKQRRFRQDKGAPQ
                     KRGVCTVVRTMTPKKPNSALRKIARVRLSNGIEVTAYIPGEGHQLQEHSVVLVRGGRV
                     KDLPGVRYHIVRGTLDTTGVANRKQGRSKYGAKRPK"
     gene            complement(3291..>5092)
                     /locus_tag="PLT08_05130"
     CDS             complement(3291..>5092)
                     /locus_tag="PLT08_05130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HNE03876.1"
                     /translation="QRSNQSTCIDQRPSVVKGQLIKAGDIIADSSSTDNGQLALGQNV
                     VIAFLSWEGGNFEDAILLSERLVQEDRFTSVHIEKYEVEARDTKLGPEEITRDIPNVG
                     DDAIKDLDENGIIRIGAEVGPNDILVGKITPKGEKELTPEERLLRAIFGEKSRDVKDT
                     SLRMPHGERGKVVDVKVFTREENSDLAAGVDMMVRVSVAQRRKITAGDKMAGRHGNKG
                     VVSKVVPVEDMPFLENGTPVDIILNPLGVPGRMNIGQVLEVHLGWAAKRLGYRAVTPV
                     FDGASEEQIEAELARAWIVDQAWKEAGVRAWDWLKEQEYDPTTIEDDDEVRRLYLEQW
                     LDKRRYDIYSLNDPAYAHRATAREWLREKGYTEPDEILLEDREVAHDTKLDDLAITAC
                     LRLWMEVNGITRRVAEDKVFETAQELAKEKGLPLPILGKQTLRDGKTGIPYDQPVTVG
                     VMTILKLHHLVEDKVHARSTGPYSLVTQQPLGGKAQFGGQRFGEMEVWALEAYGAAHT
                     LQEMLTVKSDDVQGRVNTYEAIVKGEPIEEPSIPASFRVLVKELQSLGLAVEAINENG
                     DVVKFGKDEEKTHPPKHDTGLLSLGEGQSRKKQ"
BASE COUNT         1266 a         1348 c         1186 g         1292 t
ORIGIN      
        1 catcgttccc acattcaagt gcggcttgct tctgtcaaac ttccctttcg ccatgataat
       61 actccttggg taataaccgt tacgatttca aaatttcttc agcaaccgac tgcgcaactg
      121 gagcgtagtg gtcaaattcc atactaaata cgccgcgtcc ttgggtggcg gagcgaagct
      181 gagtcgcata cccgaacatt tcacccaatg gaaccatggc acggacagcc tgagcgttac
      241 cagggcgaac atccatacct tggatcaaac cacggcggct gttaacctgt cccatcacgt
      301 cgccaagata ttcttccggc gcaactactt caaccttcat catcggttca agcaaaatag
      361 gattgccctt ctgcatacct tccttgaagc ccattgaagc ggcaagtttg aatgccattt
      421 cactggagtc aacttcatgg aatgaaccat cgaagagggt gatcttcaag tcaacgactg
      481 ggtatccagc aagcacgcca ctttcagcag cttcgcggaa acctttttca accgggttga
      541 tatattcctt cggaatagaa ccacccacga tcttgttttc gaagataata ccgctgccgc
      601 gttcaccggg ctcgagggca aatacaacgt gaccatattg acccttacca ccggattgct
      661 tggcatactt gtagttaact tccttgaccg gcttggtaat ggactcgcgg tacgccacac
      721 gcggggtacc gacattcgcc tgcactttga attcgcgcaa gagacggtca acgataatat
      781 caagatgaag ctcgcccata ccgcggatca cggtctgacc ggtactatca tctgaattaa
      841 cacggaatgt gggatcttct tcagcaagtt tacgaagcgc ctcacccatt ttttcctgat
      901 cgccagtgct cttcggctca atagcgatgg aaataacagg ctcggggaac gaaatgcttt
      961 caagaacgag cgatttatta tcacaaaggg tatcaccagt gaagctgtct ttgaagccga
     1021 gaacagcgcc aatgtcgcca gcgcgaatct cagtgatgtc ttcacgacgg tcagcatgca
     1081 tctttaacaa acgaccaatg cgttctttct tacccttggt gctattctga atagtctgac
     1141 cttggctcaa aacacccgag taaacacgaa tatacgcaag gcgtcctaca tacgggtcgg
     1201 tcacgatctt gaaaaccaaa gcgctcaaag gcgcatcatc aactgttgga agctcaaaag
     1261 tgttttcagg attaccaggt tcggatgcgg aaatggtggg cttgtctatc ggagatggaa
     1321 gatagtccac cacagcgtca agcattgcct gaacaccttt attctttaat gaagaaccgc
     1381 aaaaaacagg cgtagcctta ttggcgatca cacctttacg aagtgctgcc ttgagttcat
     1441 caactgaaat ttcctccgct tcaaggaatt tcatggttaa ttcatcgtcg agttcggcaa
     1501 tcttttcaac catcttggag cgggcttctt cagcctgagc ttttaaatca gcaggaattt
     1561 cagtaacttt aggttcttta ccgagatcat cttcccaaac aatggctgtc atagaaagaa
     1621 ggtcaacgac ccccttgaat gtggcttcaa aaccgatagg aacttgcatc gggatcggct
     1681 ttgcacccaa gcggtcaatg atggtctcaa tggtgcgttc ataagaagca ccaacacgat
     1741 ccatcttgtt aacaaaacaa atgcgtggaa caccgtaacg gtcagcttgg cgccaaacag
     1801 tttcagactg gggttccacg ccttgtacag cgtcaaacac aacaacgccc ccatcgagaa
     1861 cacgaaggga acgctgaact tcagcagtaa agtcgatatg accaggagta tcaatgatat
     1921 tgacctgata ccctttccac tcggcggaaa ctgccgcgga cacaatagta ataccgcgtt
     1981 cacgctcttg caccatccag tcagtgacgg tattcccatc gtcaacggaa ccaatgcggt
     2041 gagttaaacc ggtatagaac atgatccgct cggtggtggt cgtcttcccg gcgtcaatgt
     2101 gggcaataat gccaatattt cggtattttt ccaacggatg aacgcgtgcc attgctatct
     2161 atctacctat ctctatgttc taaaaattaa atgcggtaat gggagaaagc gcggttggct
     2221 tctgccatct tgtgggtttc atcacgctta cggatagaag cgccggtttc atttgaggcg
     2281 tcaatcaatt ccgaagcgag cttatcagcg aaagatttgc cagagcggtc acgggcagag
     2341 ccgagaatcc agcgaatcgc caaagtagtg cggcggtcag cagatacttc cattggcacc
     2401 tgataggttg caccacccac gcggcgtggg cgaacttcca tcacgggacc aacattctta
     2461 agggcagcat caaagacttc cattggattc ttttcagtgc gttctttaat aaggtccatt
     2521 gcatcataaa ccaagccaat tgccacgctc ttcttaccgc tcttaagaac gtgctggatc
     2581 atggtctgga tattaatgct attaaagcgg acatcgggaa gcagttcccg cttttctggt
     2641 tttgctctac gcatgcttaa ttcactccat caaaattact tgggacgctt cgcgccgtac
     2701 tttgagcggc cctgcttacg atttgccacg ccggtggtgt cgagagttcc gcgcacaatg
     2761 tggtagcgga cgcccggaag atccttcaca cgaccgccgc gaacgagcac gacggagtgt
     2821 tcctgcaact gatgaccctc acccggaatg tacgctgtca cttcaatacc attgcttaag
     2881 cgcacacgag cgattttgcg aagcgcagaa ttcggcttct taggggtcat ggtgcgaacc
     2941 acggtacaaa caccacgctt ttggggcgcg cctttgtcct ggcggaaacg gcgctgctta
     3001 tagctgttca atgtgaactg cagagcagga gccttacttt tggatttctt gtctttgcgg
     3061 cccttgcgga ccatttgatt tactgtcggc acgatcagcc tccgattaca aattcttcta
     3121 attgattatt tatttttgtt ttttcgcaat aaatgagacc atcaattcgt tccctgatgg
     3181 cctcatttga gctgccaatt tttttcggag cggggggtga attctgccgc tcctttttct
     3241 tgacaaaggc acaacatttt atcatgctgt accagtcaag tcaatagaaa ttactgcttt
     3301 tttctggatt gcccttcacc caagctcagc aagcccgtgt catgttttgg tggatgggtc
     3361 ttctcttcat ccttaccgaa cttgaccaca tctccattct cgttgatggc ttcaacagcc
     3421 agaccaagag attgaagttc cttcaccaac acacggaagg aagccgggat gcttggctct
     3481 tcgatgggct cacctttcac gatggcttca taggtgttga cacgaccttg aacatcatca
     3541 gacttaaccg tgagcatttc ctgtagtgta tgagcagcac catatgcttc aagcgcccac
     3601 acttccattt caccgaagcg ttgaccaccg aactgagcct taccaccgag cggctgttga
     3661 gtcaccaagc tgtatggacc agtagagcga gcatggacct tatcttccac caagtgatgc
     3721 agcttaagaa tggtcataac acccacggtc acaggctggt catatggaat gccggttttg
     3781 ccgtcacgaa gagtttgctt gccaagaata ggaagcggaa gtcccttttc ctttgcaagt
     3841 tcctgtgcgg tctcaaagac cttgtcttca gcaacacggc gagtaatacc gttcacttcc
     3901 atccataagc gcaagcaggc tgtaatcgcc aagtcatcaa gcttggtatc gtgagccact
     3961 tcgcggtctt cgaggagaat ttcgtcgggt tcggtatagc ccttttcgcg caaccattca
     4021 cgggcggtgg cacgatgcgc gtacgctggg tcattcaaac tatagatatc gtagcggcgc
     4081 ttatccaacc actgttccag atagaggcga cgaacttcat cgtcatcttc aatggtggtc
     4141 gggtcgtatt cttgttcctt gagccagtcc caagcgcgga cgccagcttc cttccatgct
     4201 tgatcaacga tccacgcgcg agcgagttcg gcttcgatct gttcctcact ggcaccatcg
     4261 aagacgggag tcacggctcg ataaccgaga cgcttggcag cccacccaag atggacttcc
     4321 aacacctgac caatgttcat acgtccagga acgccgagcg ggttgaggat aatatcaacg
     4381 ggagtaccgt tttcaaggaa aggcatatct tccacaggca caaccttgga aaccacaccc
     4441 ttgtttccgt ggcgacctgc catcttatcg cccgcggtga tcttgcggcg ctgagcaaca
     4501 gagacacgaa ccatcatatc cacgccagca gcaaggtcag aattctcttc gcgggtaaag
     4561 accttcacgt ctacaacttt accgcgctcg ccgtgaggca tgcgcaatga ggtatctttc
     4621 acgtcgcgag atttttcgcc gaagatggcg cgcaacaaac gttcttcagg agtaagttct
     4681 ttttcaccct tcggtgtgat cttaccgacg agaatatcgt tcggaccaac ttctgcaccg
     4741 atgcggatga taccgttttc gtcgaggtct ttgatagcat catcaccgac gttcgggata
     4801 tcgcgagtaa tttcttcggg tccgagtttg gtatcgcggg cttctacttc atacttctca
     4861 atatgaacgg acgtgaagcg gtcttcctga acaagacgtt cagagagcaa gatagcatct
     4921 tcgaagttac caccttccca agagaggaag gcaataacca cgttctgtcc aagcgccaac
     4981 tgaccattat cggttgagga agagtcggcg atgatatcac cagccttgat caactgcccc
     5041 tttactacag aaggacgttg gtcaatacaa gtgctctggt tggagcgctg at
//
LOCUS       DAOQJE010000141         5063 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_9693_length_5063_cov_5.091454, whole genome shotgun sequence.
ACCESSION   DAOQJE010000141 DAOQJE010000000
VERSION     DAOQJE010000141.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 5063)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5063)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5063
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9693_length_5063_cov_5.091454"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(77..1633)
                     /locus_tag="PLT08_05135"
     CDS             complement(77..1633)
                     /locus_tag="PLT08_05135"
                     /inference="COORDINATES: protein motif:HMM:NF012464.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HNE03877.1"
                     /translation="MARRAVIYIRTSSETQGANSSPVEQEADCRRLAEEKGLQVAHIY
                     RDVEKYRVGSKLVEPSGSRSDRPGLLAMLKDAARDEFDVILAWREDRLYRGLRSMLTV
                     LETVQDYKIEILLAKENFDSRIAPVRAWAAQMELDGMKERLGMGVKARLKAGKANTGQ
                     DRYGYIRISDKIHVVEEEADWVRKIFDWYNQGVSLLQIRERLIAGNAPQKGSSTPRHI
                     HWARSSIQGILESAKEYAYGHKEQSRDGETFQIPVEPILEIPTYELFKTQREKNKTNS
                     PQHIRHDYLLSGHLKCSCNLTWQAKTATHRRSRQGEWIKRKTPIGTYFCPQTHKELRA
                     SDCPKSISAKQAEAQIWEKVSEFITNPAYLLAEAEAKVSWLQKDYEQMQREELRLQKE
                     LKRLNDERQEFITIACKEQMPDEELTSRMNVLYEKERGVQRRLTTIERTKDDFIKLDL
                     EEQIKKYVAELQSEMVELIHADPQTPEERHQVFLLKKRVVDTVLEEARIDQSREIHIK
                     FRSDFTTRTN"
     gene            1838..3127
                     /locus_tag="PLT08_05140"
     CDS             1838..3127
                     /locus_tag="PLT08_05140"
                     /inference="COORDINATES: protein motif:HMM:NF024571.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNE03878.1"
                     /translation="MNENIWKSRWSKDQIKSMLLEQFDSFWSRDLGIVRTQLAEVERA
                     SGVPHAVIISGLRRAGKSTLLLQLAHRLGREQFYYLNFEDDRFLGFLAEDANDLYQAL
                     LEVFGERKIFIIDEIQNIDGWEHFVRRFMDMGFKFYITGSNASLLSRELGTRLTGRYV
                     PIELFPFSFKEFMLFRGESLPNLERMTTVDHARLQNSLQSYLKFGGIPDALKYPELPL
                     LRTLYDDVLYRDIATRYRLEAVTAIKELGFFLMSNPANLISFNKLKDQFRVGSVNTIK
                     NYIGYMENSWLLFTLNVYDYSVKRQQIAPKKIYGIDTGLINTIGFGFSPNTGKLLENL
                     VFLALRRKAKEIHYLSSPGGYEVDFYLPEQRQLIQVSGNLAQSTTRDREVRALKDAIG
                     TVKVQSAIILSDSNEDSFEINGIPIHVQSTAEWLLAE"
     gene            3439..>5063
                     /locus_tag="PLT08_05145"
     CDS             3439..>5063
                     /locus_tag="PLT08_05145"
                     /inference="COORDINATES: protein motif:HMM:NF037660.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE03879.1"
                     /translation="MSYNGFMPTPILATKLFIPPLRTNIVPRPHLTERMKEGKFHKLT
                     LISAPAGFGKTTLVSEWITGCGLPVAWLSLDEGDNDHKRFLAYLIAALRTITKNIGER
                     ASDLLDAAPFPDIESVLTSLLNEITATSDHFILVLDDYHLIESKQVDEAVSFLVEHLP
                     PQIHLVIATREDPPLPLPRLRVRGQLTELRAADLRFTPTEAADFLNRVMGLNLSAENI
                     TALETRTEGWIAGLQLAALSMQGHQDTASFIKSFTGSHRFVLDYLIEEVFQQQPERVQ
                     AFLLHTSILERMCGSLCDAILRDSSVPAQETLEHLEHANLFIVPLDSERRWYRYHHLF
                     GDLLRQRAGKNLSQAEIVELHIRASEWYEQNEVLLDAFHHALAAGDFDRAARLAETAW
                     QGMERNFQTLAWLGWVKKLPDAVIGSRPWLCVHIGWAYSDVGELEPSETYLQKAERSL
                     TDMKDWEEFKSIPSTIALIRSGNAQIEGNLPETVRYAELCLQLAPEDSLLIRAQAAIT
                     LGFTQWAVGDVEASLQAMLAWMDDMQKLGNQMYA"
BASE COUNT         1204 a         1274 c         1189 g         1396 t
ORIGIN      
        1 tttttaatga caacaaggcg gctcgaaagt gagccgcctt gttgtcattt tcggaatcag
       61 attatggagg tcaatatcaa tttgttcggg ttgtaaagtc tgaacggaat ttaatgtgta
      121 tctcccggct ttgatcaatt cgtgcttcct ccaaaacggt atccaccact cgcttcttca
      181 acagaaacac ctgatgtctc tcctctgggg tttgtggatc agcatggatc aactcaacca
      241 tctctgactg caattcagcc acatatttct tgatctgttc ttccaagtca agttttataa
      301 aatcatcctt cgttcgctca atggttgtca gcctgcgctg cactcctcgc tccttctcat
      361 acagtacgtt catccgggaa gtgagctctt catcaggcat ttgttcttta caagctatgg
      421 ttatgaattc ctgacgttca tcatttagcc ttttgagttc cttttgcagg cgtagctcct
      481 ctcgctgcat ttgttcgtag tctttctgca accaggaaac tttagcctcc gcttcagcca
      541 gaaggtaagc tgggttggta ataaattcgc ttaccttctc ccagatttgg gcttcggctt
      601 gtttggcact gatcgacttg ggacaatctg aggctcgtaa ctccttgtgc gtctgcgggc
      661 agaaataggt cccaatgggc gttttgcgct tgatccactc cccctgcctg ctacgacggt
      721 gggttgccgt ttttgcctgc caggtcagat tacatgagca ttttagatgt ccagaaagta
      781 gatagtcatg gcggatatgt tggggtgaat tggttttgtt cttttctcgt tgcgttttga
      841 acaactcata ggtggggatt tcgagaattg gctccaccgg gatttggaag gtttcaccat
      901 cacgcgactg ttccttgtgg ccataggcat actctttcgc ggattccaaa atcccctgga
      961 tgctggagcg agcccagtgg atatgccttg gtgtgctact ccccttctgt ggagcgttgc
     1021 cggcaatcag tctttcacgg atctgtaaca gcgaaacacc ctggttgtac caatcaaaga
     1081 ttttcctgac ccagtctgct tcttcctcta caacatggat cttgtcactt atgcggatat
     1141 agccatagcg atcttgcccg gtatttgctt tccccgcttt cagtcgcgct ttgacaccca
     1201 ttcccagccg ttctttcata ccgtcaagtt ccatctgggc agcccaagca cggacaggtg
     1261 cgatcctgga atcaaaattt tcttttgcca gcaggatttc gattttatag tcctgaacgg
     1321 tttccaagac cgttaacatg gaacgcaatc cacgatacaa cctatcttcg cgccacgcga
     1381 gaatgacatc gaattcatct ctggcggcgt ctttgagcat tgccagtaga ccggggcgat
     1441 ctgagcggct acccgaaggt tcgaccagtt tgctgcccac acggtacttt tctacatcgc
     1501 ggtagatatg agctacctgc aatccttttt cttctgccag gcgacgacaa tctgcttcct
     1561 gctcaactgg gctggaattt gccccttgtg tttcagacga tgtgcggatg taaattactg
     1621 ctcttcgagc catttgaata ccttcggaag aaaatatgga actaccagtt caactatacc
     1681 gaaaaaataa aataggtggt gtcgaagtgt cgctaaggaa ttcaatgtac taccgcaatg
     1741 aatccctgca taccgggtag tatggcgatc cctaccaaac tctcttcacc aaaaagtttt
     1801 cattgacaat gaaaactttt tggtttatta ttcctgcatg aatgaaaaca tatggaaatc
     1861 cagatggagc aaggatcaaa tcaaatccat gttgttggag caatttgatt ccttttggag
     1921 ccgcgatttg ggaattgtac gaacgcaact cgcggaagtg gagcgggcga gtggggttcc
     1981 ccatgctgta atcatctccg ggttacgtcg ggcagggaaa tcgacactct tgttacaatt
     2041 agctcatcgg ttggggcgag agcaattcta ctatctcaat tttgaggatg atcgcttttt
     2101 aggctttctt gcagaagatg ccaacgacct ataccaggcg ctgctcgaag tttttggcga
     2161 acgcaagatt ttcattattg atgaaatcca gaatattgat ggctgggaac attttgtccg
     2221 ccgctttatg gacatggggt tcaaatttta tatcacggga tccaacgcct ccctactaag
     2281 ccgggagctg ggaacccgcc taacgggtcg gtacgtcccg attgagttgt ttccattttc
     2341 ctttaaggag ttcatgctgt ttcgcggaga gtccctgcct aacctggagc ggatgacaac
     2401 cgtggaccat gcccgcttgc aaaattccct tcaatcctat ttaaaattcg gcggtattcc
     2461 agatgcgctc aagtatccag aactccccct cttacgtaca ctctatgatg atgttttgta
     2521 tcgagacatt gcaacacgtt atcggcttga ggcggtgacc gctatcaaag aactcggatt
     2581 ctttctgatg agcaatccag ccaatctgat atcgtttaat aaactcaaag accagtttcg
     2641 agtcggcagt gtaaatacga tcaagaatta cattgggtac atggagaaca gttggctgct
     2701 ctttacgctg aacgtctatg actactcggt aaaacgccag cagatcgccc ccaagaagat
     2761 ctatggaatc gataccggct tgatcaacac cattgggttt ggtttttcac caaacacagg
     2821 caaattgctg gaaaacctgg tttttctggc gctcaggcgg aaagcaaagg agatccatta
     2881 cctctcttcc ccaggcgggt atgaggtgga tttctatttg cctgaacaac gccagctcat
     2941 tcaggtttcc gggaacctgg ctcaatctac aacgcgggac cgggaggtac gcgctctaaa
     3001 ggatgccatt gggactgtaa aagtacaaag cgctattatt ctgtcggact ccaacgagga
     3061 ttcattcgag atcaatggca tcccgataca tgtacaatct actgctgaat ggctacttgc
     3121 agaatagatg agcctgtcta acttccccaa tttcatcccg tgcaatactc atcattgcaa
     3181 tccttctcgc aatgatgagt attgatttgc tacttcgcgt atcatgttta tgaagtctag
     3241 tgaaaataac ctcacaagca acaaatgact ttgatgtcga accttgagat tacaacacca
     3301 ccaaaatctg tccggcgggg tacagtacaa atctggcata caggtataaa tttcgtttac
     3361 gaacgtaggc ggtggatatg agaacgaagc gtcctccaaa aatggaaggc gtttttttgt
     3421 tttcaggcga gattgggtat gtcttataat ggatttatgc ccacgccgat attagccacg
     3481 aaactcttca tccctccgct tcggaccaac atcgtgccac gtccgcatct gaccgagcgg
     3541 atgaaagagg ggaagtttca taagctaacc cttatctctg cgcctgctgg ttttggcaag
     3601 acaacgctgg tcagcgaatg gattacgggt tgtgggctac ctgttgcctg gctttcgttg
     3661 gacgagggtg acaatgatca caaacgcttt ctggcatatt tgatcgccgc gttgcgcacc
     3721 attacgaaaa atatcggaga gagggcctca gacttattgg atgccgctcc attcccagac
     3781 attgaatccg ttctcacttc tcttcttaac gaaatcaccg ccacctcaga tcatttcatt
     3841 ctcgtccttg acgattatca cctgatcgaa tccaaacagg tggatgaagc cgtttccttt
     3901 ctggtcgaac atttaccgcc gcaaatacat ctcgtcattg cgacccgcga agacccgccg
     3961 cttcctctcc ctcgtctgcg tgtccgcgga cagttgaccg aactccgcgc cgccgacttg
     4021 cgttttacac ccactgaagc cgctgatttt ctcaaccgcg tgatgggatt gaatctttcc
     4081 gccgaaaaca tcaccgcact cgaaacccgc accgaaggct ggatcgcggg attgcagctc
     4141 gccgcgctct ccatgcaggg acatcaggat acagccagtt tcatcaaatc tttcacgggc
     4201 agtcaccgtt ttgtgttgga ctatctcatt gaagaagttt ttcaacagca gcctgaacgt
     4261 gttcaagcct ttcttttgca cacctccatc cttgagcgaa tgtgcggctc gctttgtgat
     4321 gccattctac gcgattcgtc agttcccgcg caggagacac tcgaacatct tgaacatgcc
     4381 aatctgttca tcgttccgtt agacagtgaa agacgctggt atcgctatca tcatctcttt
     4441 ggagatttgc tccgtcagcg cgcgggaaag aatctctcac aggctgaaat tgtcgaactg
     4501 catatccgcg ccagtgaatg gtatgagcag aacgaagtgc tgctcgatgc gtttcatcac
     4561 gcccttgcgg ctggtgattt cgaccgagcc gcacgtctcg ctgaaacagc ctggcagggc
     4621 atggaacgaa attttcaaac ccttgcctgg ttgggctggg taaagaaact gcccgatgca
     4681 gtgattggtt ctcgcccgtg gctgtgcgtc cacattgggt gggcgtattc agatgtcggt
     4741 gagttggagc cgagtgaaac atatctgcaa aaggccgagc gttcactgac ggatatgaag
     4801 gattgggaag agttcaaatc cattccgtcg acgattgccc tgatccgctc aggcaacgcc
     4861 caaatagaag ggaaccttcc tgaaacggtg agatatgctg aactctgcct acaactcgct
     4921 cccgaagata gcctgttgat ccgcgctcaa gccgccatca cgttgggctt tacccagtgg
     4981 gcagttgggg atgtggaagc gtcactgcaa gccatgctcg catggatgga tgacatgcaa
     5041 aagttgggca atcaaatgta tgc
//
LOCUS       DAOQJE010000142         5042 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_9759_length_5042_cov_5.257269, whole genome shotgun sequence.
ACCESSION   DAOQJE010000142 DAOQJE010000000
VERSION     DAOQJE010000142.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 5042)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5042)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5042
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9759_length_5042_cov_5.257269"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..434
                     /locus_tag="PLT08_05150"
     CDS             <1..434
                     /locus_tag="PLT08_05150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE03880.1"
                     /translation="MDTENVPSEWIIFNESKGEDWGGQLYTVAFAYGNESESPALEQQ
                     LIVYSDESAAITGYDEFDAYIFVEEWTVNPEITFSPSTTTDKFAAKCTDREIDHVMTK
                     ICFVLQQHANYVSALGVHMGGPITFDVLNSVLKSIDTKLNQ"
     gene            complement(477..902)
                     /locus_tag="PLT08_05155"
     CDS             complement(477..902)
                     /locus_tag="PLT08_05155"
                     /inference="COORDINATES: protein motif:HMM:NF012272.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019825 - oxygen binding [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="globin family protein"
                     /protein_id="HNE03881.1"
                     /translation="MSITLEQKRLVQETWEKVVPIAETAAELFYGRLFEIDPELKPLF
                     KNADMKEQKMKLLQMLGMAVKGLDNLDQLVPAVENLGRRHVGYGVKDSHYDTVGDALL
                     WTLEKGLGEDFTPEVMEAWTETYVTLATVMKNATETVAA"
     gene            complement(1053..1442)
                     /locus_tag="PLT08_05160"
     CDS             complement(1053..1442)
                     /locus_tag="PLT08_05160"
                     /inference="COORDINATES: protein motif:HMM:NF019928.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC domain-containing protein"
                     /protein_id="HNE03882.1"
                     /translation="MKNEFVLSETFNANAAAIYNAWLSTQGHTLMTGSPAKVNGHAKG
                     DFTAWDGYIWGTFLDLEPNKRIVQLWRTGEFPDDAEDSRVEILLEETNGKTKLTLRHT
                     HMPEDQIESYKTGWLDFYFKPMKEYFG"
     gene            complement(1480..2406)
                     /locus_tag="PLT08_05165"
     CDS             complement(1480..2406)
                     /locus_tag="PLT08_05165"
                     /inference="COORDINATES: protein motif:HMM:NF016142.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine-5'-phosphate glycosidase"
                     /protein_id="HNE03883.1"
                     /translation="MKHPHIQLSVEVSRALTMGAPIVALESTVITHGLPQPQNLELAR
                     NMEQQVRDEKATPATVAVLDGKIRLGISDEELVRLSESQSTLKVSHRDFATTIVKKVN
                     GGTTVAGTMLAANMSGIKVFATGGIGGVHKESAFDISTDLKALAETPMVVVCAGAKAI
                     LDLPATMEYLETMGVPVIGYKTDEFPAFYSRESGLNVSARLDSPEEIAEFAKAHWSLG
                     LRSAILVTNPVPEADAISKSEMEPMIAQASAEAVEKGIHGQALTPFLLGRISELTKGK
                     SLKTNLALLLNNARLAGEIANQMYTRKKEWAI"
     gene            complement(2409..3998)
                     /locus_tag="PLT08_05170"
     CDS             complement(2409..3998)
                     /locus_tag="PLT08_05170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE03884.1"
                     /translation="MNRFRRRRDEMIPRIFALTDEAVEYRSQIRNYARIVVWAVLSGF
                     TLILFFFFAETTIQRLTVLGLSLLIYLPLFSAAQTVARHKAANYLADIYEIEDETIAD
                     SFLEEVIFGEGTEKITINEGKISERDEESPLIMIGGPGYIQVNLDSVALLERVDGTPE
                     IIHPRGKPWKLGSFERIREIGKHDEPGKREYAIINLRDQFVRGLSVRSRTKDGIPLEA
                     QDIKVLFSILRKPKEEAPENDPYHFDETAVHSLVYNQTIISPSPAKTAGVSFPWDTTV
                     IPLITSEMENLITSRNLSEILASISHKEIDDLNEGEATNKQIRLEMTGEQTVPTNSNS
                     PSAPNFESRSKITAQFFSPQFKAKAAKLGVSIHWIDIGTWQLPSEMILEELKSGWKMM
                     RENVRRRNDVNRLAKRQEMKELRELVNHVIVSSFTKANNANDNRKLSDDEFSKLSKII
                     EENPNIAFSPALQQKMAQDVTLKKDAALEILKAFRKEFIAARELIQKENRSSIDKQAE
                     IARIDKALRDIDYHVFHYIKG"
     gene            complement(3995..>5042)
                     /locus_tag="PLT08_05175"
     CDS             complement(3995..>5042)
                     /locus_tag="PLT08_05175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE03885.1"
                     /translation="ASAIVTQTETEIIGAAGPGIHFTKGREKITRGEGVDLRPQWQFI
                     GPLASDQPFLNPPSPDSKKYNELQARRQQTAGQTRDGFEISPTISIKFRIKQPEEKTP
                     SESGVVSHYGYNATAVLHAVTREVIELGTSDNKRTRMEWYRLPAHLVVNLWREYVRKF
                     KLEDLFKAEGVSGLQTIEEMINRRVKRPEVVAMDDTGIPTGEALPSLEYQQLIERGLE
                     VIDVRIHNVLFDPAIEEQTIKNWNNEWTKIAKREEDLLNEREKLIETAAHNEAVKRFA
                     RLASLKFENPIAMPADAYTTLQALIEPVKETLVIESRANSQMETDIKKLDEIWKWLLV
                     SKLDKSMHRKEDES"
BASE COUNT         1164 a         1191 c         1232 g         1455 t
ORIGIN      
        1 tgatggatac agaaaatgtc ccttccgagt ggatcatttt caatgaatcc aaaggtgaag
       61 attggggcgg tcagttgtat acagttgcat tcgcgtatgg gaatgaatca gaatcgccgg
      121 cattggaaca gcaattgatc gtgtattcag atgagtctgc tgcgataact gggtatgatg
      181 aatttgatgc gtatatcttc gtggaagagt ggacggtcaa ccctgaaatc acgttttccc
      241 cgagtacaac gacagacaaa tttgcggcaa agtgtacaga ccgtgaaatt gaccatgtga
      301 tgaccaaaat ctgttttgtg ttacagcaac atgcgaacta tgtctctgct ttgggcgttc
      361 acatgggtgg acccatcacc tttgacgtgt tgaactcggt cttaaaatct attgatacaa
      421 aattgaacca ataaaaagaa ggtctcgcta aaacgagacc ttctttttta gaaccactag
      481 gcggctacag tttcagtcgc attcttcatt acagttgcta aggtgacgta ggtctccgtc
      541 caagcttcca tgacttcggg ggtaaagtct tcgccgagcc ctttttccag cgtccacagc
      601 agggcgtccc ccacggtatc gtagtgagag tcttttacgc catagccaac atggcggcga
      661 ccaagatttt ccaccgcagg cactaactga tcgaggttat ccaaaccttt tacagccatg
      721 ccgagcattt gcaataactt catcttttgt tctttcatat ctgcattctt gaacaaaggc
      781 ttaagttcag ggtcgatctc aaaaaggcga ccgtagaata gttcagcagc agtttctgcg
      841 atcggtacga ctttttccca ggtttcttgc acaagtcttt tttgttctaa tgtgatgctc
      901 atttctaaaa tactcctcta aagttaattg attaatacga gcgcgatttt ataagcatcg
      961 cttggaattg accatgtgag tttggtacta aaactcagat aggacattac tcctataaaa
     1021 aagagctgca ttttatgcag ctctttttta ttttatccaa aatattcttt cattggcttg
     1081 aaataaaaat caagccagcc tgttttatag ctctctattt gatcttcagg catatgtgta
     1141 tggcgtagcg tgagttttgt tttgccatta gtttcttcca gcaagatttc tacacgagag
     1201 tcttcggcat cgtcagggaa ttctccggtg cgccaaagtt gcacgatgcg tttgttgggt
     1261 tccagatcaa gaaaggtgcc ccaaatatac ccatcccatg cggtgaaatc acccttggcg
     1321 tgcccgttga cctttgccgg gcttccggtc atcaaggtat gtccctgtgt ggacaaccag
     1381 gcgttgtata tggctgcggc gttggcattg aaggtttcag aaagaacaaa ctcatttttc
     1441 atcctgcctc cggcgatcaa acgaatctac gctatctatc taaatcgccc attccttttt
     1501 tcgcgtgtac atctggtttg caatttcacc tgctaaacgt gcattgttca ataacaatgc
     1561 cagattggtc ttaagagatt taccctttgt caattcgcta atacgaccca gcaagaaagg
     1621 agtcagtgcc tgcccgtgga tgcctttctc tacagcttca gcagatgcct gagcgatcat
     1681 cggctccatt tcagactttg agatggcatc tgcttcggga accggattcg tcacgagaat
     1741 ggcacttcgt aaccccaggc tccagtgggc tttggcaaac tcggcaatct cttccggaga
     1801 atccaaccgc gcgctgacgt tgagtccgct ttcgcgtgag tagaaggcag ggaattcgtc
     1861 ggtcttgtac ccgatgaccg gtacacccat ggtttcgaga tattccatgg tggcgggtag
     1921 gtctaaaata gctttcgctc cagcacagac tactaccatg ggggtctcag ccaatgcttt
     1981 taaatcggtg gagatatcga aagcagattc tttatggaca cccccaatac cgcctgtagc
     2041 aaaaactttg ataccgctca tgttggcggc gagcattgtc ccggcgacgg tggtaccgcc
     2101 gttcactttt ttgacgatgg tagtggcaaa atcgcggtga ctcactttca gcgtggattg
     2161 actttcagaa agccgcacca gttcctcgtc tgagattccg aggcggatct ttccatccaa
     2221 tacagccaca gtggctggcg tcgccttttc atcgcggact tgctgctcca tgttgcgggc
     2281 gagttccaaa ttttgtggtt ggggtaaacc gtgcgtgatg acggtcgatt cgagagcgac
     2341 aataggcgcg cccatcgtga gcgcgcgaga aacttcaaca gaaagttgga tgtgggggtg
     2401 cttcattctt aacctttgat gtaatggaat acgtgatagt cgatgtcgcg cagggcttta
     2461 tcgatccttg cgatttcggc ttgtttgtct atggaagagc ggttctcttt ttggatgagt
     2521 tcgcgggctg cgataaattc tttgcggaag gctttgagaa tttcgagcgc ggcatctttt
     2581 ttgagggtga catcttgtgc catcttttgc tgcaaggcag ggctgaaggc gatgttcgga
     2641 ttttcctcaa tgatcttgga taactttgaa aattcatcgt ctgaaagttt gcgattgtcg
     2701 ttggcgttgt ttgctttggt gaaactggaa acaatgacat gatttaccag ttcgcgcaac
     2761 tctttcattt cctgccgttt cgcaagtcgg ttgacatcgt tgcgccggcg gacattttca
     2821 cgcatcatct tccaaccgct ttttaattct tcgagaatca tttcagaagg aagctgccat
     2881 gtcccaatgt cgatccaatg aatgctaacg ccgagtttgg cggctttggc tttgaactgc
     2941 ggactgaaaa attgcgctgt gatttttgaa cgtgactcaa agtttggtgc actgggagaa
     3001 ttgctgttgg ttggaaccgt ttgctcgccc gtcatttcga ggcgaatttg tttgttggtc
     3061 gcttcgcctt cgttgaggtc atcaatttct ttgtggctga tactggcaag aatttcgctc
     3121 aagttgcgcg aagtgatgag gttttccatt tcgcttgtga tgagcggaat aacggtcgta
     3181 tcccatggga aagaaacgcc agcggttttt gcaggcgaag gtgaaatgat agtttggtta
     3241 tacaccaagg aatggaccgc ggtttcatcg aaatgatacg ggtcattttc aggcgcttct
     3301 tctttgggct tgcgcaaaat gctgaaaagc actttgatat cttgcgcttc aagcggaata
     3361 ccgtctttgg tgcgcgagcg gacggaaagt ccgcgcacga attggtcgcg caggttgatg
     3421 atggcatatt ctcgtttgcc gggctcatcg tgtttaccga tctcacggat gcgctcgaaa
     3481 ctgcccagct tccacggctt gccgcgtggg tgaatgatct caggagtgcc atctacgcgt
     3541 tccagcagcg ccacactatc caagttgacc tgaatatagc caggtccgcc gatcatgatg
     3601 aggggcgact cttcatctct ttctgagatt ttgccctcgt tgatcgtgat tttctctgtc
     3661 ccttcgccga agatcacttc ttcaagaaaa ctatcggcga tcgtttcatc ttctatttca
     3721 taaatatctg ccagataatt ggcggcttta tgtcttgcaa cggtttgcgc tgcactaaac
     3781 aaaggcaggt agatcaagag acttaagccc aacaccgtca ggcgttgtat cgtagtttcg
     3841 gcaaagaaaa agaacaagat tagtgtgaat ccgcttaata ctgcccacac cacaatgcgg
     3901 gcgtagttgc gaatctggct tcgatactcg acagcctcat cggtgagagc aaagatgcgc
     3961 ggaatcattt catcgcgtcg acggcggaat cgattcatga ttcgtcctct ttgcgatgca
     4021 tggatttatc caacttgctt accaacaacc acttccaaat ttcatctaat ttcttgatgt
     4081 cagtttccat ctgactgttg gcgcggcttt caataaccaa ggtctctttt actggttcga
     4141 tcagagcttg taatgttgtg taggcatcag ccggcatggc aatcggattc tcgaacttta
     4201 gcgaagcgag ccgcgcaaaa cgtttgacag cctcattatg ggccgccgtc tcgatcaact
     4261 tctcgcgttc attcaataaa tcttcttcac gcttggcgat cttggtccac tcgttgttcc
     4321 aattcttgat ggtctgttct tcaatggcag ggtcgaacaa cacattgtga atccgcacat
     4381 cgatcacctc aagaccgcgt tcgatgagtt gctgatattc gaggctgggc aatgcttcac
     4441 cagttggaat tccggtatcg tccatggcga ctacttcggg tcttttcacc cgacgattga
     4501 tcatttcttc gatggtttgt aacccgctga caccttcggc tttgaacaaa tcttccagct
     4561 tgaacttgcg cacatactcg cgccacaaat tcaccacgag gtgagccgga aggcgatacc
     4621 attccatgcg agtacgtttg ttatccgaag ttcccaattc aatgacttcg cgcgtcaccg
     4681 catggaggac ggcggtggcg ttatagccat aatgagaaac gacgccgctt tcagaaggag
     4741 tcttttcttc tggctgctta atacggaact tgatactgat agtgggggat atttcaaacc
     4801 cgtcgcgggt ttgcccggcg gtttgttgac gccgagcctg caactcgttg tattttttcg
     4861 aatcaggcga aggcgggtta agaaacggtt ggtcactcgc caacggacca ataaactgcc
     4921 attgcgggcg caaatccaca ccttcaccgc gggtaatctt ttcacgtccc ttcgtgaagt
     4981 ggatgcccgg acctgccgcg ccgatgattt ctgtttcggt ttgggtgaca atggcgctgg
     5041 cg
//
LOCUS       DAOQJE010000143         5036 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_9776_length_5036_cov_6.361373, whole genome shotgun sequence.
ACCESSION   DAOQJE010000143 DAOQJE010000000
VERSION     DAOQJE010000143.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 5036)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5036)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5036
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9776_length_5036_cov_6.361373"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2154
                     /locus_tag="PLT08_05180"
     CDS             <1..2154
                     /locus_tag="PLT08_05180"
                     /inference="COORDINATES: protein motif:HMM:NF024583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNE03886.1"
                     /translation="SDTKVQFEYELAISGQLFWFLATLTKLDENSVFWVARDITERKK
                     SEEAIQRRNQYLAVSAEIGKLVTSILDLSTIFARTVNLITDRFNVYHAAIFIVEETGF
                     NAVLKEGTGEAGAEMKQMEHSLQLNDKSIVGSVASTGQLVVANNVLENPLYMAHPLLA
                     ETRSELAIPLKIGTRVIGVIDMQSRELNSFNEDEVSVLQTLADQVAVAIDNARSFELS
                     QQAVMEMREADRLKSQFLANMSHELRTPLNSIIGFSRVILKGIDGPVTELMQQDLTAI
                     YNSGQHLLGLINDILDSAKIEAGKMELAFDEVNIQDLTHSVLSTMSGLIKDKPIEMRQ
                     FIEQDTPTVRADAIRIRQVMINLLSNAAKFTEEGSITVNVAPYREDGKKLVKISVTDT
                     GSGISIQDQEKLFQAFSQVDASPTRKSGGTGLGLSICKQLINMHGGQIWVESKEGEGS
                     TFHFTLPLFNKENELAEEGNKVILTIDDDPQVIGLYERYLQPQGYHVHSLTDPSRAVE
                     VAKQVKPFAITLDIMMPGTDGWTVLDTLKSDSATRNIPVIICSIVEDIEKGFNLGASD
                     YLVKPILEEDLVNSLDRLNADGSIREVLVIDDNPNDLRLIGKMLNDDGRYKPILAEGG
                     RNGWTIISSGTPPHAIILDLFMPEMDGIQILEQLQADKRLRDIPTIVISGMDVTPEQK
                     NKLKEFGQRLLTKGAFNESELLTSIQRSLDRLSKK"
     gene            2177..3832
                     /locus_tag="PLT08_05185"
     CDS             2177..3832
                     /locus_tag="PLT08_05185"
                     /inference="COORDINATES: protein motif:HMM:NF012512.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HNE03887.1"
                     /translation="MSFIIKCLDCGHNAPYFPTSTTCPKCGSQWREAEYDYEMLGKTL
                     PEKLKSRQQDLWRYRELLPVRNPNISLSLGEGYTPLIRAVNLGMMLGCPNIFIKDERQ
                     GPTSSFKDRQASVTIAALKEAGITEMVAASTGNVAISYSAYASRAGMKLWAFVTSLVP
                     AVKMREIALYGSQVIKITGSYDQCKQVAAEFARQRKLYLDMGARTITSIEAMKTIAFE
                     IAEQLTNVLGPDENSQLRTPDWYVQAISGGMGPIGVHKGFREMQQMGWVDKIPAFAPI
                     QAEGCAPMVDSWKKGLAKAEPVTNPKTRIETLATGDPGRAYEFLKGHVDSSNGVFESV
                     TDEEAFRAMHVLAKMEGISAEPAAGAAFAGLFKLIRAGVIKPSDTVVVNCTGHTMPAE
                     QGILGENWARDVKFPSLAQAPQEEGLLSALTQVAPERFPKIAIVEDTLEARRLIRRIL
                     QSQGNFTILEAEDGRAGLDLVQKELPDLVILDLMMPEMDGFSVIEALRSNPETATIPV
                     IVATAKELTPDEKNRLSGHIQALLQKGDFLNDEFLDEVRSLLK"
     gene            3856..4803
                     /locus_tag="PLT08_05190"
     CDS             3856..4803
                     /locus_tag="PLT08_05190"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HNE03888.1"
                     /translation="MAKKNTAGILAAFSSALFLGMAPVFGKLAITEGFSPFAVVALRT
                     GFAAGIMLLVMMLFYRQYLYIFPVGLIGCILAGAINGLGSLFYYLALERLDASLGQLL
                     YSLYPFFVALWLILDHQPPSRLTIFRLALATIAVLLLTNVPDRPMDLTGVAMMIAAAV
                     LYAMHLPINQRVLYEVPAPTVALYTLISMSAIVIPAYLIFDRTWPASHAPWLPVAGLT
                     FVTIFSRVALFLGVKKIGGMQTALLGLAELLIAIFGSSLLLGESLTFTQWLGALGLAV
                     SLILVMYEKTAAFPAVSKSGWLSWIRPPGLPKDTFGPYE"
     gene            complement(4837..>5036)
                     /locus_tag="PLT08_05195"
     CDS             complement(4837..>5036)
                     /locus_tag="PLT08_05195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE03889.1"
                     /translation="SMKRFIEQNDRGLGIRTNVGVLLCDAEYIDIQQVLNDIDLARQQ
                     LSAGLYTNPSIFDREMLNAHR"
BASE COUNT         1347 a         1173 c         1164 g         1352 t
ORIGIN      
        1 agtgatacaa aagttcagtt tgagtatgaa cttgctatat ctggacaact tttttggttc
       61 ctcgcgaccc tgacaaaatt agatgagaat agcgtcttct gggtggcgag agatattaca
      121 gaacgcaaaa aatctgaaga agcgattcaa cgcagaaatc agtatttggc ggtatcagct
      181 gaaatcggta aattggtcac ttctatcctc gacctgagca ccatctttgc ccgtaccgta
      241 aacctcatca cagatcgttt caatgtgtac catgccgcaa tttttattgt agaagaaact
      301 ggctttaatg cggttctgaa agaaggaacc ggagaagctg gcgctgaaat gaaacaaatg
      361 gaacattctt tgcaattgaa cgacaaatcc attgtaggct cggttgcatc tactgggcaa
      421 ttggtggttg caaataatgt tcttgaaaat ccgctttaca tggcacatcc attattggca
      481 gagacacgtt cagagcttgc cattccgctc aagatcggta cacgtgtaat cggtgtgatt
      541 gacatgcaat cacgagaact aaactcattc aatgaggatg aagtttcggt tcttcaaacc
      601 cttgcagacc aagtggctgt tgctatcgac aatgcccgct cctttgaact ctcacaacaa
      661 gccgttatgg aaatgcgcga ggcagatcgg ttaaaaagcc agttcctcgc aaatatgagt
      721 catgagttgc gtacgccttt gaattccatc attggttttt cacgcgttat tctaaaaggt
      781 attgacggac ctgtcactga gttaatgcag caagacctaa ccgcaattta taactctggt
      841 caacacttgc ttggtctcat caacgatatc ctcgactctg ccaagatcga agccggcaaa
      901 atggaattgg cgttcgacga agttaatatt caagacctga ctcacagtgt attgtctacg
      961 atgagcggtt tgatcaaaga caaaccaatc gagatgcggc agtttatcga gcaagatacg
     1021 ccaaccgtcc gcgccgatgc gattcgtatc cgtcaagtca tgatcaacct gctttctaat
     1081 gcagctaaat tcacagagga aggctctatc acggttaacg ttgctcctta tcgtgaagat
     1141 gggaagaaac tagtcaaaat cagtgtgacc gatacaggct ctggtatttc aatccaagac
     1201 caagaaaaac tcttccaagc cttttctcaa gtagatgcct ctcctacccg caaaagcggt
     1261 ggcacaggct taggtctttc catctgtaaa caattaatca atatgcatgg tggacagatc
     1321 tgggtagaaa gtaaagaagg tgaaggaagt acgttccact tcactttgcc gctcttcaac
     1381 aaagaaaacg aattggcaga ggaaggcaat aaggttatct taactattga tgatgaccca
     1441 caagtgattg gtttgtatga acgttacctc cagccgcaag gctatcacgt ccactcatta
     1501 acagaccctt cgcgagcagt agaagttgcc aaacaagtaa agccttttgc aattactctt
     1561 gatattatga tgcccggcac cgacggctgg actgttttgg atacgctcaa atccgactct
     1621 gcaacccgga atattccggt tatcatatgt agtatcgttg aagatattga aaaaggtttc
     1681 aaccttggcg cttccgatta cttggtaaag cctattctag aagaagactt ggtcaattcg
     1741 ttagatcgct tgaatgcgga tggctccatt cgtgaagtgc ttgtgattga tgacaacccc
     1801 aatgacctgc gtttgattgg caaaatgctg aatgacgatg gacgatataa acccatcctt
     1861 gccgaaggtg ggcgcaacgg ttggacgatt atctcctctg gtacaccgcc tcatgccatc
     1921 atcttggatt tgttcatgcc agaaatggac gggattcaaa tccttgagca gttacaggca
     1981 gataagcgtt tacgtgatat acccaccatt gtaatcagcg gtatggatgt cacgcctgaa
     2041 caaaagaata agctcaaaga gtttggtcaa cgcttattaa ccaagggagc ttttaacgaa
     2101 agtgaattac taacttcgat tcaacgctcg ctggatcgct tgtcaaagaa ataatcgaag
     2161 tgtacaggag aattaagtgt catttattat taagtgccta gattgtgggc ataacgcgcc
     2221 atactttcca acctccacaa cctgccccaa gtgtggaagt caatggcgcg aagcggaata
     2281 cgactatgaa atgcttggca aaaccttgcc agaaaagtta aagtcgcgcc agcaagacct
     2341 ttggcggtat agagagctac ttcctgttcg taacccaaac attagtttat cgctaggtga
     2401 aggctacacc ccattgattc gtgccgtcaa cttgggaatg atgttgggct gcccaaatat
     2461 ctttatcaaa gatgaacgac aaggacccac ttcttctttc aaagaccgtc aggcatcagt
     2521 gactattgcg gcactcaaag aagccggtat cactgaaatg gtagctgcat cgactgggaa
     2581 tgttgccatt tcatattctg cctacgcttc tcgagcaggg atgaagcttt gggcatttgt
     2641 taccagcctt gttccagcag tcaaaatgcg tgagatcgct ttgtatggca gccaagtcat
     2701 caaaatcact gggtcatatg accaatgcaa acaagtggct gctgaatttg cacgtcaacg
     2761 caaactgtat ctggacatgg gtgcacgtac tatcacatcc attgaagcga tgaagaccat
     2821 tgcatttgaa atcgcagaac agcttaccaa cgttctcggt cccgatgaaa actctcagct
     2881 gcgcaccccg gattggtatg tgcaagccat tagcggcggg atgggaccga ttggcgtcca
     2941 taaaggcttt cgtgaaatgc aacaaatggg ctgggtggac aaaatccccg cttttgctcc
     3001 cattcaagca gagggctgtg cgcccatggt agatagttgg aaaaagggtc ttgcaaaggc
     3061 agaaccggtt accaacccaa aaactcgcat tgaaaccctt gccacaggcg accccggacg
     3121 tgcctacgaa tttttgaaag ggcatgttga ttcaagtaac ggtgtttttg aaagtgtgac
     3181 cgatgaagaa gctttccgtg ctatgcacgt gctcgcgaag atggaaggta tttccgcaga
     3241 gcctgccgct ggagctgcgt ttgctggctt gtttaaatta attcgtgcag gtgtgatcaa
     3301 gccaagtgac acggtggttg ttaactgcac tgggcatacc atgccagctg aacaaggtat
     3361 tcttggtgag aactgggcac gagatgtgaa gttcccgtct ttagctcagg ctccacaaga
     3421 agaaggctta ctatctgcgt taacgcaagt agcacctgaa cgcttcccca agatcgccat
     3481 cgtagaagac acgcttgaag cacgccgctt gattcgccgc attttgcaat cgcaagggaa
     3541 tttcacaatt cttgaagcag aagatgggcg tgccgggtta gaccttgttc aaaaagaatt
     3601 gcccgacctt gttattctcg acttgatgat gccagagatg gacgggttct ctgttatcga
     3661 agcattacgt tcgaatcctg aaaccgcaac catccctgtg attgttgcga cggcaaaaga
     3721 gttgacaccc gatgaaaaga atcggctcag cggacacatc caggcattgc tgcaaaaagg
     3781 tgattttctc aacgacgaat ttctggacga ggtcaggtct ctcttaaagt aactattcaa
     3841 taaaggctgg caagattggc aaaaaaaaat acagccggga tccttgcggc gtttagctcc
     3901 gcccttttcc ttggcatggc acctgtgttc ggtaaacttg ccatcacaga aggtttttca
     3961 ccatttgcgg ttgttgcttt acgaactggg tttgcagcgg ggatcatgtt actggtcatg
     4021 atgttgttct atcgccagta tctctacatc tttcctgtag gtctaatcgg ctgtatcctt
     4081 gctggtgcca ttaatggact agggtcgctg ttttactacc ttgccttgga acgtttagac
     4141 gccagcttgg ggcaactgct ttattctctc tatccgttct ttgttgcact ttggttgatt
     4201 cttgaccatc aaccgcccag ccgtctgaca attttccgcc tcgcacttgc aaccatagct
     4261 gttctgctcc ttacgaatgt tccagaccgc cccatggacc taaccggtgt agctatgatg
     4321 atcgcagccg ctgtgctata tgctatgcat ctcccaatta atcagcgggt gctctacgaa
     4381 gtccctgcgc cgactgtcgc gctctacaca ctgatatcta tgagcgcgat tgtgatccct
     4441 gcttacttaa tctttgaccg cacttggcca gcatcgcatg cgccttggct accagtcgca
     4501 gggctaacct ttgtgaccat tttctcgcgt gtggctttat tccttggcgt gaaaaagatc
     4561 ggtggcatgc aaaccgcatt gctaggtttg gcagagttac tgatcgctat ttttggtagc
     4621 agtttactgc ttggagaaag ccttacattt acccaatggc ttggtgcgct tgggctggca
     4681 gtgagtctta tccttgtaat gtatgaaaaa accgctgcat tcccagccgt ttccaaaagc
     4741 ggatggctct catggattcg cccacccggg ttacctaaag acacatttgg tccatatgaa
     4801 taaaaagagg tcgacgtcat gtcgacctct ttttattcag cgatgtgcgt tcaacatttc
     4861 tctgtcaaaa attgatggat tggtatataa ccccgcactc agttgctgac gtgccaagtc
     4921 gatatcgttc aaaacttgct gaatgtctat atattcggcg tcacacaaca aaacaccaac
     4981 atttgtacga atgcctaatc cgcggtcatt ttgttcgatg aaacgcttca tactat
//
LOCUS       DAOQJE010000144         5033 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_9784_length_5033_cov_6.810366, whole genome shotgun sequence.
ACCESSION   DAOQJE010000144 DAOQJE010000000
VERSION     DAOQJE010000144.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 5033)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5033)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5033
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9784_length_5033_cov_6.810366"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(113..1075)
                     /locus_tag="PLT08_05200"
     CDS             complement(113..1075)
                     /locus_tag="PLT08_05200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE03890.1"
                     /translation="MKSLNSALLLSMVLILAISTSCVQAGEDTKIPSLPASITVTSTK
                     VPTALPTDTPTFISTLPIEQAQANLLELLSNNASCRLPCFLGITPGKTTYTEARNILY
                     PFSSISISMNLSGDNNADSAWLTYDEGDIRTFAELSYFYNNKGTVNSLMFKAGEYKET
                     AESRSPVYNSKIFGNRLHLYMLSGILSEFGKPTSVVIHTSGEQITGSGGFDILLLYPN
                     EGIFAHYTTQMETTGTNARGCPANAQVELHLSPSGNIEAFTKSLSETTLGGMFEGLEI
                     FDNPSWKSIEKVTTMSLDQFHETFYLPTDKCIETPLKEWYVPDN"
     gene            complement(1265..1951)
                     /locus_tag="PLT08_05205"
     CDS             complement(1265..1951)
                     /locus_tag="PLT08_05205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE03891.1"
                     /translation="MSTTITIQPFERAYCLSLINFLHTVLPNFGIHEIDVMYGLAWGN
                     EIFNWKTRSIELSSLLNEISDIESQGFGNLGEDELHIIIKSHNLKIVLCHETDIHVEF
                     DIATPLLEQMLNFLSSSFQTITQETEPYTSPIFFSDKPTFSVSSILSAEKLESSIINL
                     VQWNKILELPPCKLIRIMGYDSSQSGLLEIDQSNEIIKVKGWHSSNIRVYCDKYLLYD
                     TKELSKEGRV"
     gene            complement(2442..2648)
                     /locus_tag="PLT08_05210"
     CDS             complement(2442..2648)
                     /locus_tag="PLT08_05210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE03892.1"
                     /translation="MTKKRLTKRAPDAGDSAHISSSFTRLIIFLDGRLRRPRPSAGNA
                     NRWVDSLRLSLEKHSPTENVWIWL"
     gene            complement(2645..4969)
                     /locus_tag="PLT08_05215"
     CDS             complement(2645..4969)
                     /locus_tag="PLT08_05215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE03893.1"
                     /translation="MQNQAIKGKRMIAESIANISLSQLNITTRSYNPLKTAGISTIGQ
                     FLNAYDDSSIDSVLTPKTYKVIYQHIQVLIETSNQGIPDWLAYSRQLQIPVIPESYVS
                     ESSSNNILKDIPSIVNQVLGDERRWYIIERRFEFNNTAKLTLQDLGESFGGVSRQRID
                     QIWDESIIKLRSVLIEHNFKGEPYRIHPEILEFIEKIISFLNENVSSGILENRLFDSL
                     EHSFDLQIKTARPVLFLLLELCGFDCLDFGVSDLNPIWGKFDKTNRRIYEIAIPQIDK
                     LLTVETVHPQKEFDVLRLINKSMPKNSKLLPSDIKMAIELCSSVEKRDDGLYWGKFEY
                     LKGRGNQAERLLLENAPHTMSIDSIVREINHRLVLLGTKKANHRNVANQISKNERFAN
                     IEKSGQWGLSTWEHIDTSSIVELMEEFFARKNKPATTDEIYEYVSSKRPVEKSSVTMY
                     LTTKEQFSKHDRLHWKMKKWNEPDGEMWGIDQVASFVEDFFKKNKAKVLEYKIVKQAL
                     MDEAGLTSKQAQGMLNINPVIETYWDDHRKIFATLQENYHDKLLHVKTFGLRKGNTTL
                     GEKISERIKEIINASPDKQILLSELVKRLRKEFKFNNRPEPYGYVQRAEFVEVIELPN
                     QRQKNCRIKVNENLQFPQIDKISNIELKDKVKRAMGFLNLENVDIGLFLLSKEFESTL
                     LMYLLQAEKRGKITNPTPGKWKSLDEMINFISKEGLVTDKATLHYLRQKRNDRAHGTM
                     PSIEERQLMMNNLQVVTNLYIDYIKFFDDRTNAL"
BASE COUNT         1418 a          988 c         1012 g         1615 t
ORIGIN      
        1 tagaaaaatg cctgagattg ccgcagaatc cccagcgtca ggtgcacgct ttgttaggcg
       61 gtggttgacc gaagcctcat caccattgtt taataggctc tgacactgtt ggttaattat
      121 ctggcacata ccattctttt aatggcgtct caatacattt atcggttggg agataaaaag
      181 tttcatgaaa ttgatctagc gacattgttg ttactttttc tattgatttc caagaaggat
      241 tgtcgaaaat ttctaatcct tcaaacatac ctcctagagt tgtctcggac aatgatttag
      301 taaaggcttc aatatttcca gatggagata gatgtaactc tacttgtgcg ttggcagggc
      361 aaccacgcgc attggttcct gtagtttcca tttgtgtagt gtaatgagca aatatcccct
      421 catttggata gaggagaaga atatcaaatc cgccactacc tgtaatttgt tctccactgg
      481 tatgaatcac caccgaggta ggttttccaa actcggatag gataccggac aacatatata
      541 agtgcaaccg attaccaaaa atttttgaat tatagacagg cgaacggctc tcagcagttt
      601 ctttgtattc tcccgcctta aacattagac tgttaacagt gcctttgtta ttataaaagt
      661 aactcaattc ggcgaatgtc cgaatgtcac cttcatcgta tgtaagccaa gcactatctg
      721 cattattatc gccactcagg ttcattgaaa ttgagatgct actaaagggg tatagaatat
      781 tacgtgcttc agtataagtt gtttttcctg gtgtaatacc taaaaaacaa ggtaaacgac
      841 aactagcatt attggaaagc aattccaata gatttgcttg ggcttgttct ataggcaagg
      901 ttgaaataaa agttggcgta tcagttggca aagcggtcgg gacttttgtg gacgtaaccg
      961 tgatagacgc tggcaaggaa ggaatttttg tatcttcacc tgcttgtaca caactggtgc
     1021 taattgcaag aattaacacc atactaagca ataaggctga attgagggat ttcatatttg
     1081 ccttggagac tcagaccaaa ttcaataatt tcatacgtag ccgcctaacg gcttgcgtta
     1141 cctgcgctgg ggcggggacg gcgaagccgt ccagccagaa aaatgataag gcgtaggaaa
     1201 atgcttgaaa atggcgcaga atccccagcg tcaggtgcac gctttgttgg ctggctgtta
     1261 atttttagac tcttccttct tttgacaatt cttttgtgtc ataaagtaga tacttgtcgc
     1321 aatatactcg aatattgctt gaatgccaac ctttgacctt gattatttca ttactttggt
     1381 caatttccaa taatcctgat tgagaagagt cgtagcccat aattctaatt aatttgcagg
     1441 gcggaagttc taatatttta ttccattgca ccaaattgat aatcgaggac tctaattttt
     1501 cggcggacaa aatacttgaa acactaaagg taggtttatc tgaaaagaaa attggggatg
     1561 tgtatggctc tgtttcttgt gtaatggttt ggaatgatga agataaaaaa ttaagcattt
     1621 gttcaagaag aggagttgca atatcgaact caacatgaat atctgtttcg tggcatagga
     1681 cgattttaag attatgggac ttaataatga tatgaagttc gtcttcacct aaattaccaa
     1741 agccttgact ttcgatatca gaaatctcgt tcaagagact acttaattca atgctacgag
     1801 ttttccaatt gaatatttca ttaccccaag ccaatccata cattacatca atttcatgga
     1861 tgccaaaatt aggaagcact gtatgaagaa aattgatcaa agaaaggcaa tacgctctct
     1921 cgaaaggctg gatggtaatt gttgtagaca tttttatttt atagcaaaag aattgattat
     1981 gttatgcaca aagccagcca acggcctgcg tgacctgccg atgggggagg ggcggaaaag
     2041 gccagagcgt ccgataaggc cttagaggtg aaaactgcca gggcttcgat aaagaccctg
     2101 aaggtcaggt ccacgctttg ttgggcggct ccttggtagc ggggaaaggc tgaggcgtag
     2161 gcctctatta tatgacggta aagcgaaaaa aaagcaggcg gaataaaaca aaaagagaaa
     2221 aacaaggtaa agcctgcccc ctgtttaccc cgtagggtgt attttttcgc ctgacgcagt
     2281 tcttacccct gctcagaggc tcattgtcaa cggtaggcag cacgattttt ttacggcgtc
     2341 gaggccgtaa aaatcgtgcg ccggttccta ccgttgatct ttgagatggg caggggtagc
     2401 gtcgggcagg cgaaaaaaac atagtcgtgt agcctcttgt cttatagcca tatccaaacg
     2461 ttttcggtgg gggagtgttt ttcaaggctg aggcgtaggc tgtccaccca acggtttgcg
     2521 ttacctgcgc tggggcgggg acggcgaagc cgtccatcca gaaaaatgat aaggcgtgtg
     2581 aaactgcttg agatgtgcgc agaatcccca gcgtcaggtg cacgctttgt taggcgtttt
     2641 tttgtcataa ggcatttgtc ctatcatcga agaactttat gtaatcaatg taaagattgg
     2701 taacgacttg cagattgttc atcataagtt gacgctcttc aatagatggc atagttccat
     2761 gagcgcggtc atttcgcttt tgcctgagat aatgtaaagt ggctttgtct gtaaccaaac
     2821 cttcttttga aataaagttt atcatttcat caaggctttt ccactttcca ggtgttgggt
     2881 ttgtaatctt tccacgtttt tcagcctgta ataaatacat caacaaagtc gattcaaatt
     2941 ctttgctaag taaaaacaac ccaatatcta cattttcaag attcaaaaaa cccattgctc
     3001 ttttgacttt atcttttaac tcaatattgc taattttatc aatctgagga aattgtaaat
     3061 tttcattgac cttgatgcgg caattttttt gcctttgatt tggtagttca attacttcta
     3121 caaattctgc tcgctgaaca tagccgtatg gttcggggcg attgttaaac ttaaattctt
     3181 tcctaagcct ttttacaagt tcagataaca agatttgttt atcaggtgac gcattgataa
     3241 tttccttgat gcgttccgaa attttctctc ctaacgtcgt gtttcctttt cgtaacccaa
     3301 atgttttaac atgcaaaagt ttatcgtggt agttttcttg taatgtggcg aagatttttc
     3361 tgtggtcatc ccaataagtt tctattacag ggttaatatt caacattcct tgtgcttgtt
     3421 tagatgtaag tcctgcttca tccattaaag cctgcttgac aattttgtac tccaaaactt
     3481 tcgctttgtt tttcttgaaa aaatcttcca caaaactggc aacttggtct ataccccaca
     3541 tttcgccatc tggctcattc cattttttca ttttccagtg cagcctgtca tgctttgaaa
     3601 actgctcttt tgttgtcaag tacatagtta cagaactttt ttcaacgggg cgtttgctcg
     3661 aaacatactc gtaaatttca tctgtcgttg caggtttatt ttttcttgcg aaaaactctt
     3721 ccattaactc aacaatactg ctcgtgtcaa tatgctccca tgtacttaaa ccccattgac
     3781 cacttttttc gatatttgcg aatctttcat ttttggatat ttgattggca acatttctat
     3841 gattcgcttt ttttgtaccg agcaaaacta atctgtgatt gatttctctt acaattgaat
     3901 caatactcat agtatgtggc gcattttcca aaagcaacct ttctgcttga tttccacgcc
     3961 ccttaagata ttcaaacttg ccccaataca aaccgtcatc acgtttttcc acactgctac
     4021 atagttcgat tgccattttt atatctgatg gtagcagttt agagttcttt ggcattgatt
     4081 tgtttatgag gcgtaagaca tcaaactctt tttgagggtg aacagtctca acagttaaca
     4141 gtttatctat ttgaggtatg gcaatctcat aaatgcgacg atttgtttta tcaaattttc
     4201 cccagattgg attcaaatct gaaacgccga aatccaaaca atcaaaaccg cataattcga
     4261 gtaaaagaaa cagcacaggt cttgctgtct taatctgtaa atcgaaagaa tgttcaaggc
     4321 tgtcaaaaag tcgattttct aatattccac ttgaaacatt ttcattcaaa aaggaaataa
     4381 tcttttcaat aaactctaat atttcagggt gaatacgata aggctctccc ttgaaattgt
     4441 gttcaatcaa tacacttctt aacttgatta ttgactcgtc ccaaatttgg tctattcttt
     4501 gtctgcttac tccaccaaaa gactctccca aatcttgtaa cgtaagtttg gctgtattat
     4561 tgaactcaaa tcttcgctcg atgatatacc atctgcgctc atcgccaaga acttgattaa
     4621 ctattgaggg aatatccttc aaaatattat tagaggatga ttcggaaaca taactttctg
     4681 gaatgacagg aatttgtagt tggcgtgaat aggcaagcca atcgggaatg ccttgattag
     4741 aagtttcaat taatacctgt atatgctggt aaattacctt gtaagtttta ggagttaaaa
     4801 ccgaatcaat tgaagagtcg tcgtaggcat ttaaaaattg cccaatcgtt gatattcccg
     4861 cagttttaag gggattataa gaccgtgttg ttatattcaa ttgcgataga ctgatattgg
     4921 caatgctttc tgcaatcatt cttttccctt ttattgcttg gttttgcaag acaaacgcct
     4981 aacggtttgc gttacccgcg ctggggcggg ggacggcgaa gccgtccgac tgg
//
LOCUS       DAOQJE010000145         5002 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_9871_length_5002_cov_8.145138, whole genome shotgun sequence.
ACCESSION   DAOQJE010000145 DAOQJE010000000
VERSION     DAOQJE010000145.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 5002)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5002)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5002
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9871_length_5002_cov_8.145138"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..523)
                     /locus_tag="PLT08_05220"
     CDS             complement(<1..523)
                     /locus_tag="PLT08_05220"
                     /inference="COORDINATES: protein motif:HMM:NF025890.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c3 family protein"
                     /protein_id="HNE03894.1"
                     /translation="MHQRIKLRTPHIWTVSLLLGLGLFAFALAALTTTAQAAPSLQPA
                     AQDQKPNNDFCLACHQEEGIDKTLGSESLPVTINPTEFGLSVHAEEGVLCVDCHVDIS
                     DYPHPDVKSKSVRDFQMTFADSCKECHEDQFTDAHDSVHQTAMDEGDPNAPLCADCHN
                     PHTQGRIIGETSGE"
     gene            complement(717..2873)
                     /locus_tag="PLT08_05225"
     CDS             complement(717..2873)
                     /locus_tag="PLT08_05225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE03895.1"
                     /translation="MSLKKFLVLLGVLVVLGAVLAACGGTQPTQAGTETATEEAPAAP
                     IPDTPYLADWQGSAHANVADEPFRHWDDAAENPDGVPTSCAKCHTTDGYRDFLGADGS
                     EAGKVDVAVAADHAQGVQCVACHNAVTMTKTTVMFPSGVEITAGDDSRCMECHQGRES
                     KVSVDAQIEKFAATDPDAVVAPIKDDQGNDVFFGFRNVHYYAAAATLYGGMTHGGYEY
                     DGLTYDAKNTHTDGYASCTGCHDPHTLEVKVDQCAFCHEDVATVEDLKNIRMVSSAPD
                     YDGDGNVEEGMYYEIEGLQAALLAEIQTYAADKGGAAIIYDSASYPYWFIDTNANGAA
                     DEGEVAFPNAYSTWTPRLLKAAYNYQVSMKDPGAFAHGNKYIVQLLFDSIADLGGDTS
                     KLARTDAGHFAGNTEPFRHWDKDEEGNPVYEVEAACAKCHSAAGLPQFLEYGANLPVE
                     SANGFMCSTCHNEEAWPERYAVASVTFPSGSTVSFGGKDADGNFVADDSNLCIECHQG
                     RSSTPTLNKALTGKEEDTPDPKIRFSNIHYFAAGATIFGADVQGAYMYEGKEYVGQNM
                     HADEAGKMNKCQDCHDVHALEPKVEACETCHDTTDPTAVRESDVDYDGDGDVTEGIKG
                     EIDTMTEALYAAIQAYAEGHGGAIEYKATAYPYFFGADGKGYAAWTPRLLKAAFNYQY
                     VQKDPGAYVHNPKFVIQFLYDSIQDLGGDVSAFTRP"
     gene            complement(3097..3480)
                     /locus_tag="PLT08_05230"
     CDS             complement(3097..3480)
                     /locus_tag="PLT08_05230"
                     /inference="COORDINATES: protein motif:HMM:TIGR02605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HNE03896.1"
                     /translation="MPTYDFICNTCEKRFEVFLTYSEYGKKTVECTHCNSTDVRRRMT
                     KVRIAKTDEGRLEHMAGDLGSLEGMENDPAAFARRMKKMGKEMGEELPAEFDEVVDRL
                     EAGQSPEEIESAMPDLSGGDELSKE"
     gene            3540..4154
                     /locus_tag="PLT08_05235"
     CDS             3540..4154
                     /locus_tag="PLT08_05235"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HNE03897.1"
                     /translation="MTEFELRKPNPLLIVISGPSGVGKDSVVQRMKDRGFPFHFVVTA
                     TTREKRANETHGVDYFFLSKDEFAHMIEEDELIEYAIVYGDYKGIPKEQVRNAFASGK
                     DVVMRIDVQGAESVRKLAPEALLIFITCESEEELERRLRERKTESPDSLSLRIATARK
                     ELQRLEAFDYVIVNHDFHLDDTVDKVRAIIDAEHLKVHHRMVTL"
     gene            4151..4951
                     /locus_tag="PLT08_05240"
     CDS             4151..4951
                     /locus_tag="PLT08_05240"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF013827.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HNE03898.1"
                     /translation="MIQPPASYSDPSLGQQPEPIPTRPPTYVRMPTLQPIVTYALIGV
                     TIFVYLLQVLSSVLFGDSSYGIDWVTLYGARISDAIYAGQLWRLFTPMFLHGSIPHIF
                     FNMYALFSVGSLLERHFGHGRFLLLYVLGGFAGNVLSFLFTQGYSVGASTAVFGLIAA
                     EAIFFYQNQKLFGSHAKQAISNAIFIIAINLFLGLAPSIDTWGHIGGLLGGAMFAWFA
                     GPKWTVVGMPPEYHLDDERESREIITGALLVLLIFGGLTVWAFIQHVG"
BASE COUNT         1181 a         1224 c         1276 g         1321 t
ORIGIN      
        1 gctcgccgct ggtttcgcca atgatgcgtc cctgagtatg ggggttgtga cagtcggcgc
       61 agagtggggc gttggggtcg ccttcatcca tcgcggtttg gtggacacta tcatgtgcat
      121 cggtgaactg atcttcgtga cattctttgc aggagtcggc gaaggtcatt tgaaaatcac
      181 gaacggattt tgattttacg tctgggtggg gatagtcgct gatatctaca tggcaatcta
      241 cacagagaac gccttcttca gcgtgaaccg ataaaccaaa ttcagtgggg ttgatggtta
      301 ctgggagcga ttcgctaccg agggttttgt cgatgccttc ctcttggtga caggcaagac
      361 aaaaatcgtt attcggtttt tggtcttgtg ctgcgggctg aagagaaggg gcggcttgcg
      421 cggttgtggt gagtgctgct agagcgaagg cgaaaagccc aagtccgaga agcaggctca
      481 cggtccagat gtgaggcgtg cgaagtttga tcctttggtg catcacattc tcctttttaa
      541 aattcggaca atttacatat tatattcccg attacaaacg tgggggaatt gccccaattg
      601 taagtggggc atgcgcccca ttacagtgct aaatatcacg gttttggtgt gattaagaat
      661 tggattaaaa agaaaatggc ggatgttgcc atccgccatt ttttattact tggagcttac
      721 gggcgtgtga acgcgctcac atcaccgccc aggtcctgaa tggagtcata caggaactgg
      781 ataacgaact tgggattgtg aacgtacgca cccgggtctt tctggacgta ctggtagttg
      841 aaggcagcct tgagcaagcg aggagtccac gctgcatatc ccttgccatc cgcaccgaag
      901 aagtacgggt aagcagtggc tttgtattca atggcgccgc catgtccttc agcatatgcc
      961 tgaattgcag catagagggc ttcggtcatg gtgtcgatct cgcccttgat accttcggtt
     1021 acatcgccgt cgccatcata gtccacatcg ctttcgcgga ctgctgttgg gtcggtggtg
     1081 tcgtgacagg tttcacaggc ttcgactttg ggttcgagag cgtgaacatc gtgacaatcc
     1141 tggcatttgt tcatcttgcc agcttcgtca gcatgcatgt tctgacctac atattccttg
     1201 ccttcgtaca tgtaagcgcc ctgaacatca gcaccaaaga tggtagcgcc ggcagcaaag
     1261 tagtgaatat tgctgaagcg gatctttgga tcaggagtat cttcttcctt accagtgagt
     1321 gccttgttca aagtcggtgt agaagagcga ccctgatggc attcgataca aaggttggag
     1381 tcgtcggcga cgaagttgcc atcggcatct ttaccaccaa agctgacggt tgaaccactg
     1441 gggaaggtca cagaagcaac ggcgtagcgt tcgggccaag cttcttcatt gtgacaggtg
     1501 gagcacatga aaccgttggc agattccaca ggcaggttgg cgccgtattc aaggaattgc
     1561 ggcagaccag cagcgctatg gcacttggca caagcggctt ctacttcgta gacaggatta
     1621 ccttcttcat ccttatccca gtggcggaaa ggttcggtgt tgccagcgaa gtgacccgca
     1681 tcggtacgag cgagtttgct ggtatcgcca ccgaggtcgg cgatcgagtc gaagagcaac
     1741 tgaacgatgt acttgttgcc gtgggcaaac gcaccaggat ccttcataga gacctgatag
     1801 ttataagcag ccttgagcaa acgaggagtc caggtggaat acgcattcgg gaaggcaact
     1861 tcaccttcat cggctgcgcc attggcattt gtgtcgataa accaataggg ataggatgct
     1921 gaatcgtaga tgatcgcagc gccaccctta tcagcggcat atgtttggat ctcagccaac
     1981 aaagcagctt gtaagccttc gatctcataa tacatacctt cttcaacatt gccatcgcca
     2041 tcatagtcag gagcggatga aaccatgcgg atgttcttga gatcttccac ggtggctaca
     2101 tcttcgtgac agaaagcgca ctggtcaact ttgacttcga gggtatgagg atcatgacaa
     2161 cctgtgcagg aagcataacc atcagtgtgg gtgttcttgg catcgtaggt caagccatca
     2221 tattcatatc caccatgggt cataccaccg tagagggtgg cagctgcagc atagtagtga
     2281 acattgcgga aaccaaagaa cacatcattg ccctggtcat ccttgatcgg agcaacaacg
     2341 gcatcggggt cggtggcggc aaacttttca atctgcgcat ccacactgac cttggactca
     2401 cgtccctgat gacattccat acaacggcta tcatcaccgg ctgtgatttc aacaccggag
     2461 gggaacatga ccgtggtctt ggtcatggtt acagcgttgt gacaagccac gcactgcacg
     2521 ccctgggcat ggtcggcagc aacggctaca tccactttac cggcttcgga accatcggca
     2581 ccgaggaaat cacgataacc atcggtggta tggcacttag cgcaggaagt gggaacgccg
     2641 tcaggatttt cggcagcgtc atcccagtga cggaaaggtt cgtctgctac attcgcgtga
     2701 gcagagcctt gccaatcagc taagtatgga gtatctggta ttggagcagc gggagcttct
     2761 tccgtggctg tttcagtacc tgcttgagtg ggttgggtgc cgccacaggc cgctaaaacc
     2821 gcgccgagca caaccaaaac cccgagcaaa actagaaatt ttttgagact cattattcat
     2881 tctccttaga tcagtgtaaa aaagctggta aatatcggcg tgttcttgaa aatatgcctc
     2941 ctttggagaa aatgactgct ttgacatttt ttagcagata tgagaaattt tacttaagtg
     3001 ggagattaat gcacagtaaa aataagcatc ccttaatgtg acaaaagtca caacaattta
     3061 gataagatta gtgaaattat acgtcgggga aagggtttat tccttagaaa gctcgtcgcc
     3121 cccactaagg tctggcatgg ctgattcgat ttcttcgggg ctttgcccag cttccagcct
     3181 atccaccact tcgtcaaact cggctggcaa ctcttcgccc atctctttcc ccatcttttt
     3241 catcctacga gcaaatgcgg cagggtcatt ttccatccct tccaaactgc ctaggtcacc
     3301 agccatgtgt tcaagtctgc cttcgtccgt ttttgcaatg cggaccttcg tcatacggcg
     3361 gcgcacatct gtgctgttgc aatgagtaca ttcaaccgtt ttctttccat attcgctata
     3421 tgtcaaaaaa acttcaaagc gcttttcaca ggtattgcag ataaagtcat aagtaggcat
     3481 agactgattt tacagaatca agccagaggt tggcaagagg cgtttggtac aatacgcaga
     3541 tgactgaatt cgagcttcgt aaacccaatc cgctattgat cgtcatctct ggtccttctg
     3601 gtgtaggcaa agactctgtg gtgcaacgca tgaaagaccg cgggtttccc ttccactttg
     3661 tggtgactgc caccactcgc gagaagcgag ccaatgagac ccatggcgtg gattatttct
     3721 tcctctcaaa agatgaattt gcgcacatga tcgaagaaga tgagttgatc gaatatgcca
     3781 tcgtctatgg cgattacaaa ggcattccca aagaacaggt gcgcaatgcc ttcgccagcg
     3841 gcaaagatgt ggtcatgcgc atcgacgtgc aaggagctga gtcggttcgc aagcttgcgc
     3901 ctgaagcttt gctcattttc atcacgtgcg aaagcgaaga agaattagaa agacgtttgc
     3961 gcgaacgcaa gaccgaatcg ccagactccc tttctctacg cattgccacc gcccgcaaag
     4021 aactgcaacg gctcgaagcc tttgactatg tgatcgtcaa tcatgatttt catttggatg
     4081 acactgtgga taaagtccgc gcgatcattg atgccgaaca tttgaaagtg caccaccgca
     4141 tggtgacctt gtgattcaac ctcccgcctc ttattcggac ccgtcactgg gtcaacaacc
     4201 cgaaccgatt cctacccgtc cgcccactta cgtgcggatg cccaccttgc aacccatagt
     4261 cacctatgca ctcattggtg tgaccatttt cgtttatctt ctacaagttc tcagttctgt
     4321 cctttttggt gactctagct acggcattga ttgggtgact ttatatggcg cccgcatcag
     4381 tgacgcaatc tatgccgggc aactttggcg tttgttcacg cccatgttcc tgcatggctc
     4441 catcccgcat attttcttta atatgtatgc gcttttctcg gttgggagtc tgctggaacg
     4501 tcactttggg catggacgct tcttgttgct gtacgtactc ggtggttttg ccgggaatgt
     4561 attgtctttt ctctttaccc aaggctattc cgtcggtgct tccacagcgg tatttggtct
     4621 cattgccgca gaagccatct tcttttacca aaaccaaaag ttattcggtt cacatgccaa
     4681 gcaagccatt agcaatgcta tctttatcat tgccatcaat ctcttcctcg gcttggctcc
     4741 cagcattgat acgtgggggc acattggggg tctgctcggc ggggcaatgt ttgcctggtt
     4801 tgcaggtccc aagtggacgg tggtcggtat gccgcctgaa tatcatctgg atgacgaacg
     4861 agaaagtcgt gagatcatca ctggtgcatt gcttgttctg ctcatctttg gcgggctaac
     4921 cgtttgggca ttcattcaac atgttgggta acaaataata aaaaagcccg tcgtcaagac
     4981 gggctttttt gattcaatgt tt
//
LOCUS       DAOQJE010000146         4996 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_9890_length_4996_cov_7.922688, whole genome shotgun sequence.
ACCESSION   DAOQJE010000146 DAOQJE010000000
VERSION     DAOQJE010000146.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 4996)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4996)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4996
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9890_length_4996_cov_7.922688"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..380)
                     /locus_tag="PLT08_05245"
     CDS             complement(<1..380)
                     /locus_tag="PLT08_05245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE03899.1"
                     /translation="MPLHIIGMNIEILDLGLIDYEQAWKLQDEYAGEIAKGLRPPTLL
                     LLEHPHVYTFGRKGNASNLLWNENQLKEKGIATYWVDRGGDVTYHGPGQLVGYPLIPL
                     GRLNQDNRLPEADYVGYIRKLETM"
     gene            complement(404..1798)
                     /locus_tag="PLT08_05250"
     CDS             complement(404..1798)
                     /locus_tag="PLT08_05250"
                     /inference="COORDINATES: protein motif:HMM:NF013714.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HNE03900.1"
                     /translation="MRTTFTKLENFGHSLEAPAYVLKPRNSEEIYSAFKQAKKMGLTV
                     TARGAGRSYNDASLNGGGIVLNMTGMDQILEWDPATGLVRCEPGVTLEKLWQQVEPDG
                     WWPPVVSGTMRTTLGGCLSANIHGKNNFKMGTIGEHVVEFTATLPTGAEVTCSPKKNA
                     DLFYSMISGYGMLGIFTSVTMQMKRLHSGLLVVDALPVHNLREHLSALQDGAPHNDYI
                     VGWLETFASGKALGRGQIHAARYLHEGEDPNPQETMKLKNQILPPMIFGVFPKSILYQ
                     FMKPFANNLGWRLVNIAKYVASLPKHTFRQSHAAFHFLLDYVPNWELSYGRGGLIQYQ
                     SFLPKETAESAWREVLSLSQKRGMPSYLGVTKRHRPDKFLLTHAVDGFSLALDFKVSD
                     STRPKMAAMLKEFDKIVLAHGGRFYFAKNGETTAETAQAFYGEETVKKFKKLKKRCDP
                     DGLLESDLYRRIFG"
     gene            complement(1807..2589)
                     /locus_tag="PLT08_05255"
     CDS             complement(1807..2589)
                     /locus_tag="PLT08_05255"
                     /inference="COORDINATES: protein motif:HMM:NF012334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HNE03901.1"
                     /translation="MSDLKPATPLNPRRRAIVVGASVGMGAALSKKLAKEGYVVALVA
                     RQKEKLNELCAEINESAGEVCAFAYPHDVSNYKEAPELLKNIVAEIGGLDTFIFMAGV
                     NYPPGGNDIYNFENDRQMVEINLIGAMAWLSPVAEMFQAAKYGQIVGVGSVAGDRGRV
                     GNPGYNTSKAGLATYLEALRNRLTRHGVNVLTVKPGFVKTEMLKAAVGPTPFAIPVEK
                     AVDDIYKALQKRKQSIYTPFFWQYIMLIIRHIPSFIFRRLSF"
     gene            2738..4000
                     /locus_tag="PLT08_05260"
     CDS             2738..4000
                     /locus_tag="PLT08_05260"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor domain-containing diguanylate cyclase"
                     /protein_id="HNE03902.1"
                     /translation="MNSNHGKHGSVFTRAWDIISRDDTIPPEEKKAILRLIQVIVEAR
                     EQASDKRVGDDEEKSITHEVISKHSLMMTVKHQADELDALRRISLNLTSNLDLQKVLD
                     ILVTESMGLIKNANNVTIFLYERGELEFGAALDDKGERNIPVSKPRKNGLTYSVATSG
                     QQIIVEDMKSHPFYQNTSLNLTGSIIGVPLKFKDEVVGVMNLSKKVVGKFTRSELRLI
                     NLLADQAAVAIFNASMYKRLAQMANTDSVTGLPNRRALDERLQEEWRLAEQTKFPFAV
                     VMMDLDGFKAVNDTFGHNIGDDLLYSLFNFLAQRMRSSDFLARYGGDELTLVMRNTDA
                     EAAQSVMKKILELMQDYHFPFPKGKDMKLGITAGIAIYPTHANTPSDLIRAADSALYH
                     AKKYARGEYVVAKAITGRLNPVQVKDGS"
     gene            4072..4995
                     /locus_tag="PLT08_05265"
     CDS             4072..4995
                     /locus_tag="PLT08_05265"
                     /inference="COORDINATES: protein motif:HMM:TIGR00147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family lipid kinase"
                     /protein_id="HNE03903.1"
                     /translation="MPRKVKLILNPMADMGRAWKTANALRPIAQEFQGELTWSGTVYP
                     THAIELARQAAEEGYDMVIAMGGDGTVHEVMSGLMQVPAEKRPVMGVVPIGSGNDFAY
                     SIGIHQKPDHALAHALKAENIKPVDIGLMTDEHGRKEYFDNTLGIGFDAVVTIRSHKL
                     PIVKGFLMYLTAVIQTILLNHNPAKLNIETDHQSWSASALMLTLCNGPREGGGFMLSP
                     KSKNDDGTMEFLIVNKVSRAMMFRLVPEFMSGTHMRFKQIQMGEFKRFSLTSDSPLYI
                     HADGEIFTSFGSNLKKVAFEILPHALKVVHG"
BASE COUNT         1312 a         1079 c         1266 g         1339 t
ORIGIN      
        1 aacatggttt cgagcttgcg aatataccca acgtaatcgg cttcgggtaa acgattgtct
       61 tggttcaatc tccctagtgg aattaacgga tagcccacca gttgaccagg tccatgatag
      121 gttacatcac caccacggtc tacccagtaa gtggcgatgc ctttttcttt gagctgattt
      181 tcattccaaa gtaagttgct ggcattgcct ttacgcccaa aagtgtacac atgtggatgc
      241 tcaagcaata aaagagttgg cgggcgcagc cctttcgcaa tttcccccgc gtattcatcc
      301 tgaagcttcc aagcttgttc gtagtcgatt aaacctaaat ctaaaatttc gatgttcata
      361 ccaataatat gtaggggcat gatttatcat gcccctgttc aaattatcca aatattcttc
      421 gatacaaatc tgattccaat aaaccgtctg ggtcgcagcg tttctttaat tttttgaact
      481 tcttgacagt ctcttcgccg taaaaagcct gcgctgtttc agcggttgtt tcaccattct
      541 tggcaaagta aaaacgccca ccatgagcta ggacgatctt atcaaattcc tttaacatgg
      601 ctgccatctt cggacgagtg gaatcggaca ctttgaaatc caacgcgagc gagaaaccat
      661 ctacagcgtg ggtgagcaaa aatttatcgg ggcggtggcg cttggtcaca ccgaggtacg
      721 agggcatacc gcgcttttgt gaaaggctca acacttctcg ccatgcagac tccgcggtct
      781 ctttggggag gaaggattga tattggatca agccgccgcg tccatatgaa agctcccaat
      841 tcggcacata atctaacaag aaatgaaatg ccgcatgtga ttggcggaag gtatgttttg
      901 gaagggatgc cacatacttt gcgatattca ccaaacgcca accgagattg ttcgcgaacg
      961 gtttcatgaa ctgatataag atggatttcg gaaagacgcc aaagatcatg ggcggaagaa
     1021 tctggttctt caacttcatc gtctcttgcg ggttcgggtc ttcgccttcg tgcaaatagc
     1081 gtgcggcatg aatttgtccg cgccctaatg ctttaccgct tgcaaaggtt tccagccagc
     1141 caacaatgta atcgttatgc ggggctccgt cctgcagagc agaaaggtgt tcgcggaggt
     1201 tatgtacggg gagcgcatcc acaactaaaa gtccggagtg gaggcgtttc atttgcatgg
     1261 ttacggaagt gaagatgcca agcatcccat atccgctgat catggagtag aacaagtccg
     1321 cattcttttt gggcgaacaa gtcacttccg cgccggtggg tagtgtggct gtaaactcca
     1381 ccacatgttc accgatggtt cccatcttaa aattattttt gccgtggatg tttgcactga
     1441 gacaaccgcc caatgttgtg cgcatcgtac cagagacgac gggcggccac cagccatctg
     1501 gttcgacttg ttgccaaagt ttttcaagcg tgacgccggg ctcgcatcgc accaagccgg
     1561 tagctggatc ccattccaga atttgatcca tgccggtcat gttgagcaca attccgccac
     1621 cgttgaggga agcatcgtta taacttcgcc cggctccacg agctgtgacg gtaagcccca
     1681 ttttctttgc ttgcttgaat gcagaataga tttcttcgct gttgcgaggt tttagaacat
     1741 acgccggggc ttcaagagaa tgaccaaaat tttcgagctt cgtaaaagta gtacgcatga
     1801 ggttcgttag aaagagagac gtctgaatat gaaagagggg atatgccgaa tgataagcat
     1861 gatgtattgc cagaagaaag gcgtatagat ggattgcttt cgtttctgca gagctttgta
     1921 aatgtcatcc actgcttttt cgaccgggat tgcgaacgga gtgggaccta ctgcggcttt
     1981 gagcatttcg gttttcacga agcctggttt gacggtgagc acattcaccc catgacgggt
     2041 gaggcggttt cgtaaggctt ctagataagt tgccaaccca gctttggatg tgttgtagcc
     2101 ggggttgcct actcgtccac ggtcaccagc aacggagccg acaccaacaa tttgcccata
     2161 ttttgcggct tggaacattt ccgcgactgg gctgagccat gccatagccc caatcaggtt
     2221 gatttcgacc atttggcggt cgttctcgaa attgtagatg tcgtttccac cgggcgggta
     2281 atttacccca gccatgaaaa taaaagtatc taaaccgcca atttcagcga ctatattctt
     2341 taagagttct ggagcttctt tatagtttga aacatcgtgc gggtaagcaa aggcacatac
     2401 ttccccagca gattcgttga tttcggcgca gagttcattc aatttttctt tttgacgggc
     2461 aactaaagca acgacatacc cctctttggc aagtttttta gagagcgcag ctcccattcc
     2521 aacagaagcg ccgaccacaa tggctctgcg gcgcggattt aatggtgttg caggttttaa
     2581 gtcactcaca atgacctcgt attaagtatt tattcatgtc attctaacac agcaataaat
     2641 gacctttcgg gtcgaaatag aatatgtata tgaaagtgta taatagtaca aaggatgtct
     2701 cgacagttaa tgctttttag gcataattct aaaaaacatg aatagcaatc atggcaagca
     2761 cgggtcggtc tttacgcgag cgtgggatat tatctctcgt gatgatacga ttccgccaga
     2821 agagaaaaaa gccatccttc ggctcattca ggttattgtt gaggcacgtg agcaggcttc
     2881 tgataaaaga gtaggggatg atgaagagaa atccattacc catgaagtga tctccaaaca
     2941 ctcgctgatg atgaccgtca agcatcaggc agatgaactg gatgcgttaa ggcgcatcag
     3001 cctgaatttg acctcgaact tggatttgca aaaagtgttg gatatcctcg tgacagagtc
     3061 gatggggttg atcaagaatg caaacaatgt gaccatcttc ttatatgaac gcggcgagct
     3121 ggaatttggg gctgcattgg atgacaaagg ggaaagaaat atcccggttt caaaaccacg
     3181 aaagaatggg ttgacgtatt ctgtcgccac ctcgggacag cagattattg ttgaagatat
     3241 gaaatctcat ccgttttacc agaacacctc attgaattta acagggtcaa ttattggtgt
     3301 gccgcttaaa tttaaagatg aggttgtggg ggtgatgaac ctttcgaaaa aggtagtggg
     3361 aaaatttacc cgctctgaat tgcgtcttat taacttattg gcagatcagg cggcggtagc
     3421 catcttcaac gctagcatgt ataaacgctt ggcacaaatg gcgaatacgg atagtgtgac
     3481 gggattaccg aaccgccgtg ccttggatga acgtttgcaa gaagaatggc ggcttgccga
     3541 acaaaccaag ttcccctttg cggttgttat gatggacttg gatggtttca aagcggtaaa
     3601 tgacaccttt ggtcataata ttggggatga tctgttgtat tctttgttca attttctagc
     3661 acagcgaatg cgcagttcag attttcttgc gcgttatggc ggtgacgagt taactttggt
     3721 catgcggaat acagatgccg aagcggctca atctgtgatg aaaaagattc tcgaactcat
     3781 gcaggattat cacttcccat ttccaaaagg gaaggatatg aaattgggca ttacagcagg
     3841 gattgcaatt tacccgaccc atgccaatac cccaagtgac ttgatccgtg cggcagattc
     3901 ggctttgtat catgccaaga aatatgcccg cggcgagtat gtggttgcca aggcgatcac
     3961 gggtaggttg aatccggttc aagtgaaaga cggttcctga agcgagacgc aactcaaagt
     4021 tacgaagttg aggcggtggg gttatatcct taaaccaata aggagagaca aatgcctcgt
     4081 aaagtcaaat tgatcctcaa ccctatggca gatatggggc gggcgtggaa gaccgccaat
     4141 gcgttgcgac cgattgctca ggaatttcaa ggtgaactta cctggagcgg gacggtgtac
     4201 ccaacacacg cgattgaact tgcgcggcag gctgccgaag agggatatga tatggtgatt
     4261 gccatgggcg gggatggtac ggttcatgaa gtgatgagcg ggttgatgca agtgcctgcc
     4321 gagaagcgcc ctgtgatggg tgttgtgccg attgggtctg gaaatgattt tgcgtattct
     4381 attggtatcc accaaaagcc agaccatgcg cttgcccatg ccttgaaggc agaaaatatt
     4441 aaacctgtgg atatagggct gatgacggat gaacacgggc gcaaggaata ttttgataat
     4501 acccttggca tcggttttga tgcggtggta actatccgtt cgcataagtt gccgattgtc
     4561 aaaggatttc tgatgtactt gaccgcggta attcaaacca ttcttttgaa tcataaccct
     4621 gcaaaattga atattgaaac tgatcatcaa tcgtggagcg caagcgcttt gatgctgacg
     4681 ctgtgtaacg gtccgcgaga gggtggtggg tttatgcttt cgccgaaatc aaagaacgat
     4741 gatggcacaa tggaattttt gattgtcaat aaggtttcgc gtgcgatgat gtttcgattg
     4801 gtgcccgagt ttatgagcgg aacgcacatg cgttttaaac agatccaaat gggcgaattt
     4861 aagagatttt cgctcacttc tgattctccg ctttacattc atgcagacgg tgagattttt
     4921 acaagttttg gcagtaatct aaaaaaggtg gcattcgaaa ttctgcctca tgcattgaaa
     4981 gtggtgcatg ggtaaa
//
LOCUS       DAOQJE010000147         4996 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_9891_length_4996_cov_6.325845, whole genome shotgun sequence.
ACCESSION   DAOQJE010000147 DAOQJE010000000
VERSION     DAOQJE010000147.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 4996)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4996)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4996
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9891_length_4996_cov_6.325845"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1237
                     /gene="miaB"
                     /locus_tag="PLT08_05270"
     CDS             <1..1237
                     /gene="miaB"
                     /locus_tag="PLT08_05270"
                     /EC_number="2.8.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HNE03904.1"
                     /translation="EEADVIVLNTCVVRQSAEDKAMGRVSSLAPLKKKNPNLVINLMG
                     CMVGVRGAEKLKAKLPYVDVFSPPSDPGPLISHLTQGEIQSLEDAETTRRFLTMDEEL
                     ILPVAERGKLVMAHVPIVYGCSHACTFCIIPYKRGVERSRPVGDIVSEVRSLAKQGVK
                     EITLLGQIVDRYGKDIPDGPNLAALLRILHEVEGIERIRFLTSHPNYFDDDLINAIAE
                     LPRVMPHIELPIQAGDDTVLENMKRGYTQQQFRDLVAKIRQHIPNCSIATDIIVGFPG
                     ETEEQFMETYRVLSDLKLDVAHLARYSIREGTVATRRMVDDVPEEEKMRRLHMLDDLQ
                     EQIVSEINKKYLGQTVDVLFEENVKGRWKGRTPTNKLVFVQSEEDLRGKLLPVTVTWT
                     GPWSMQANLVKQPAELISL"
     gene            1299..2216
                     /locus_tag="PLT08_05275"
     CDS             1299..2216
                     /locus_tag="PLT08_05275"
                     /inference="COORDINATES: protein motif:HMM:NF015509.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HNE03905.1"
                     /translation="MSFIELFNTFANNLLPIMLIAAAGYILGKTLTVDSRTIGRMVFY
                     IFSPLLVFNLMVTSQLNLKQALTTVGYTVAVIAIMGLLAFIFGKIFQLSRPHLLAVII
                     TVAFGNTGNYGLPLVKFAFGDEALAVASIFYVTTTILFNTVGVVIASLGHLDLKSAIL
                     GVFKLPILYAVTIALILKGFGIAIPLPLSRTIEIAANGAIPAMIILLGLELTRIEWTQ
                     SLRATGVGVAAKLLIGPLVGLLLASLFGLQGHTRQGSVIEAAMPAAVATTVVATEYKL
                     EPSLVTAIVFFGTILSPITLTPLIVFLTR"
     gene            2222..3454
                     /locus_tag="PLT08_05280"
     CDS             2222..3454
                     /locus_tag="PLT08_05280"
                     /inference="COORDINATES: protein motif:HMM:NF024876.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase MA family metallohydrolase"
                     /protein_id="HNE03906.1"
                     /translation="MSATRQRILQVFMVTLLVATLSLKPNQAQAQEGIVVENPNAIPD
                     FGKTITFTAKIKASNPIQQASLLFRGVNEETTRVETLQVAEDGSVQFIYDASQNVFPP
                     FSWVVYWFQATLSDGKTYTSESNRVQYVDNRFPWRDVTQANVTIHWYAGDDAFGASAL
                     DSAGAGMLGMKDFIPLSLTEPIHIYIYSNVQDLGDTLMLGGREWAGGHAHADLGVVLV
                     AIAPGANQFTEMESLIPHELAHVMLYRALGDTYYKQPAWLIEGIASMVELYPNPDYAR
                     ALEIASVNDTLIPFSDMCASFPADSGSAFLAYAQSQSFVTYIRDSFGVSGLSRLISTY
                     GDGFDCDLGITKALGASLNQLDSRWRENVLGQNQVGAALRNLLPFLLLLLLVLIVPIW
                     GAVDVMRRRRKKYAAKSK"
     gene            3435..3737
                     /locus_tag="PLT08_05285"
     CDS             3435..3737
                     /locus_tag="PLT08_05285"
                     /inference="COORDINATES: protein motif:HMM:NF012911.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HNE03907.1"
                     /translation="MQPNPNEPVRVHIWVKGRVQGVGFRAHVEYSARQIGGLSGWVRN
                     VGDDTVEAIAEGQREQVERLIEAMKQGPRMSRVDEAKIDWESPTGEYNGFRVKSSA"
     gene            3741..>4996
                     /locus_tag="PLT08_05290"
     CDS             3741..>4996
                     /locus_tag="PLT08_05290"
                     /inference="COORDINATES: protein motif:HMM:NF028237.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase N-terminal 7TM
                     domain-containing protein"
                     /protein_id="HNE03908.1"
                     /translation="MFSNPYSPYLFTSAITSFVMAMLLWRRRIAPGATPLSVLLGLTS
                     VWALASALLLESSSTLLRELWFDILSISAMMTTPMLLIFCLEYTERQNFLSKRLLLFV
                     YLFPILNAILCVTTNYHHLFYAEVDFSYQPANFLALVPGPFYWIYTVYSYGIALFIFV
                     LILTNFFQTSSTYRKQTATILLGLLFPFVGNVIYTLLLGVGKGAFDPTPMLFTVMGFF
                     LVCGLLWFQLFDVIPVARHTLVEHMQDGVIVVDVKNRIVDVNPSALKWLNWEGKSSPI
                     GKNVEELLTSWFEQFANLPIDLYVQTEVHKKGTPGLYFDLRVEPIYNRQNVLTGRLIV
                     FRDITRQKMSEKALLEAHDRLRLHLKEIELLQSELREQVIRDPLTGLFNRRYMEETLE
                     REISRAIRENISIGVCMADIDQFKSF"
BASE COUNT         1225 a         1275 c         1167 g         1329 t
ORIGIN      
        1 cgaagaagca gacgttatcg ttttaaatac ctgtgtcgtt cgtcaatcgg ctgaagacaa
       61 agcgatggga cgtgtctcat cactcgctcc attgaagaag aaaaacccaa acctcgtcat
      121 caacttaatg ggatgcatgg tcggcgtgcg tggcgcggaa aagctcaaag ctaaacttcc
      181 ctatgtagat gtgttctctc ccccatcaga cccgggtccg ttgatttcgc acctcaccca
      241 aggtgaaatc caatcccttg aagatgccga aaccacccgc cgcttcctca ccatggacga
      301 agaactgata ctgccggtgg ctgaacgcgg caaactcgtt atggcgcacg ttccgattgt
      361 atacggctgt tctcacgcct gcaccttttg cattattccc tacaagcgcg gcgttgaacg
      421 ctctcgccct gtcggcgata ttgtgagtga agtccgctcc ttggcaaagc aaggcgtgaa
      481 ggaaatcacc ctactcggtc agatcgtaga tcgctacggc aaagacattc ccgacggtcc
      541 taaccttgcc gcactcctgc gcattctgca tgaagtagaa ggcatcgaac gtatccgctt
      601 cttaacctca catcccaact actttgatga tgacctcatc aacgccattg ccgaattacc
      661 gcgcgttatg ccgcatatcg agcttcccat tcaagctggc gacgacaccg tgctcgaaaa
      721 catgaaacgc ggatacaccc aacaacaatt ccgtgacctt gtggcaaaga tccgccaaca
      781 catcccgaac tgttccatcg caaccgatat catcgttgga ttccccggcg agaccgagga
      841 acagttcatg gaaacctacc gcgtcttatc ggacctcaag ctggatgtag cccatttagc
      901 ccgctactcc atccgcgaag ggaccgttgc cactcgccgc atggtagacg atgtaccaga
      961 agaagaaaag atgcgccgcc tgcacatgct cgacgatttg caagaacaga ttgtcagtga
     1021 aatcaacaag aagtaccttg gtcaaaccgt agatgtactc ttcgaagaaa acgtcaaagg
     1081 tcgctggaaa gggcgcacgc caaccaacaa actggtcttt gtgcaatcgg aagaagacct
     1141 acgcggcaaa cttctacccg tcaccgtcac ttggacgggt ccgtggtcaa tgcaggcgaa
     1201 tttggtaaaa caaccggctg aattgatttc gttgtaattt tgtaggggcg gatcgcgatc
     1261 cgcccctacc tttttcgggg gttataattt cattcaacat gtccttcatc gaactcttca
     1321 acacctttgc aaacaacctg ctgcccatca tgctcatcgc tgcggctgga tacatcctcg
     1381 ggaaaacgct caccgtcgac tcgcgcacca ttgggcgtat ggtcttttac atttttagcc
     1441 ccttactcgt tttcaactta atggtcacca gccagttaaa cctgaagcaa gccctcacta
     1501 cagtgggata cacagttgcc gtgattgcaa ttatggggct gcttgctttc atcttcggga
     1561 aaatctttca acttagccgc ccacacctct tggcggtcat tatcacggtc gcatttggaa
     1621 acacgggcaa ctatggcttg cccttggtta aattcgcatt tggcgatgaa gcattggcag
     1681 ttgcttctat tttttatgta acaaccacca ttcttttcaa taccgttggg gtggtcattg
     1741 catcgctcgg gcatttagat ttgaaatccg ctatactggg agtattcaag ttgcccattc
     1801 tttacgctgt gaccattgca ttaatcctca aaggctttgg catcgccatt ccactgccgc
     1861 tgtcacgcac catcgagatc gccgcaaacg gagccattcc tgccatgatc atcttgctcg
     1921 gcttggagct cacccgcatc gaatggacgc aaagcctgcg cgcgactggc gtgggcgttg
     1981 ctgctaagtt attgatcgga ccgttggttg ggttattgct ggcaagcctg ttcgggttgc
     2041 aagggcatac ccgtcagggc agtgtcatcg aagccgccat gcccgccgcg gtagcaacaa
     2101 ccgttgttgc taccgaatac aaacttgaac cgtctcttgt gacagcgatt gttttcttcg
     2161 gcaccatact gagtccgatt acccttacac cgttgatcgt tttccttacg aggtaatgaa
     2221 catgtccgcc acacgtcaaa ggattttgca agtcttcatg gtcacacttc tcgtggcgac
     2281 cctttctctg aaaccgaacc aagcccaggc gcaagaagga atcgttgtcg aaaatccaaa
     2341 tgcaatacct gactttggaa agacgatcac cttcacggca aagatcaaag catccaatcc
     2401 tattcaacaa gcatcgctct tgttccgcgg ggtcaacgaa gaaacgaccc gtgtagaaac
     2461 cttacaagtt gcagaagatg gctcggtaca attcatctat gacgcttcgc aaaatgtttt
     2521 ccctccattc agttgggtcg tctattggtt tcaagcaaca ttaagtgacg gcaaaactta
     2581 caccagcgaa tcaaatcgcg ttcaatatgt tgataatcga ttcccatgga gagatgttac
     2641 gcaagccaac gtcaccattc attggtatgc cggcgatgat gcttttggtg caagcgcatt
     2701 agatagtgcc ggtgcaggca tgttggggat gaaagatttt attccgctct cacttaccga
     2761 acccattcat atttatatct actccaacgt ccaagacctt ggcgatacgc tgatgctcgg
     2821 cgggcgcgaa tgggcaggcg gacacgctca tgcagacctt ggcgtggtat tggttgcaat
     2881 tgcgcccggc gccaatcaat tcaccgaaat ggaatctcta atcccacatg aacttgcaca
     2941 tgtcatgcta tatcgcgcgc ttggcgatac ttactacaaa caacctgcct ggctgattga
     3001 aggcattgcc tccatggtgg agctttaccc caaccccgat tatgcgcgtg ctttggaaat
     3061 tgccagtgtg aatgacacct tgattccatt ttcggatatg tgcgcctctt tccccgccga
     3121 ttctgggtca gcgtttctgg catatgctca atctcaatcc tttgtcacct acatccgcga
     3181 ctcgttcggg gtctcgggtc tctcacgcct gattagtact tacggtgacg gcttcgactg
     3241 tgacctcggc attaccaaag ccttaggtgc ttcgctcaac caattggatt cacgttggcg
     3301 tgaaaatgtg ctcgggcaaa atcaagtagg cgcagcgtta cgaaacttat tgcccttcct
     3361 acttttactt cttcttgttt tgatcgtacc aatctgggga gccgttgatg tgatgcgccg
     3421 aaggagaaag aaatatgcag ccaaatccaa atgaaccagt ccgtgtacac atttgggtga
     3481 agggacgggt gcaaggggtt gggtttcgtg cacacgtaga atattcggcg cgccaaattg
     3541 gagggttaag tggctgggta cgcaatgtag gggatgatac agtggaagcc atagctgaag
     3601 gtcaacgaga acaggttgaa cgtttgatcg aggcgatgaa acaaggtccg cgcatgtcac
     3661 gggtggatga ggcaaaaatc gattgggaat cgcccacagg ggagtacaat gggtttagag
     3721 taaaaagcag cgcataagtt atgttttcaa acccatactc cccctaccta tttacctcag
     3781 ccatcacctc atttgtgatg gcaatgttgc tgtggcgtcg acgcatagct ccgggggcga
     3841 ctccattatc cgtcctcttg ggtctcactt cagtttgggc gttggcatcg gctttgttgc
     3901 tagagtcgtc cagtacgtta ctcagggagt tatggttcga tatattaagc ataagcgcaa
     3961 tgatgaccac ccctatgctg cttatctttt gtttggaata cacagagcgc caaaacttct
     4021 taagtaaaag attgttactc tttgtctatt tatttccgat tcttaatgcc attctgtgtg
     4081 tcacaacgaa ttaccatcac ctgttctatg cagaggttga tttttcatac cagcctgcta
     4141 attttcttgc tttggttccg ggtccatttt attggatata caccgtgtat tcctacggca
     4201 tagcgctttt tatatttgtt cttatcctta cgaatttttt tcaaacctct tcaacttatc
     4261 gcaagcaaac agccactatt ctacttgggc tattgttccc attcgttggg aacgtgattt
     4321 acactctgct tcttggggtt ggcaaaggtg cttttgaccc cacacctatg ttgtttactg
     4381 tgatgggatt cttccttgta tgtgggttgc tctggtttca gttattcgat gtgatcccag
     4441 ttgcgcggca tacactggtt gaacacatgc aagatggcgt gattgtagtg gatgtaaaaa
     4501 atcgcatcgt agacgtgaat ccatccgcct tgaaatggct gaattgggaa ggcaaatcat
     4561 cccccattgg gaaaaatgtt gaagaacttc ttacttcgtg gtttgaacaa ttcgcaaact
     4621 tacccatcga cctttatgtt caaacagaag tacataaaaa aggcactcca gggttgtatt
     4681 ttgacctgcg tgtagagcct atctacaata ggcaaaacgt tctcacgggt cggctgatcg
     4741 tgtttcggga tatcacccgc cagaaaatgt ctgaaaaggc tttattggaa gcccatgacc
     4801 gcttaagact ccatctgaaa gagattgagc ttctgcaaag tgagttgcgc gaacaagtca
     4861 tccgcgaccc gctgacgggg ttgttcaacc gtcgttacat ggaagaaaca ttggagagag
     4921 aaatctcgcg agccattcgc gagaacattt caattggggt ttgtatggca gatattgacc
     4981 agttcaagtc tttcaa
//
LOCUS       DAOQJE010000148         4983 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_9932_length_4983_cov_5.650365, whole genome shotgun sequence.
ACCESSION   DAOQJE010000148 DAOQJE010000000
VERSION     DAOQJE010000148.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 4983)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4983)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4983
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9932_length_4983_cov_5.650365"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..760)
                     /locus_tag="PLT08_05295"
     CDS             complement(<1..760)
                     /locus_tag="PLT08_05295"
                     /inference="COORDINATES: protein motif:HMM:NF015715.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HNE03909.1"
                     /translation="MQTTTVNRLPRNAILFILLAVLIAVASSLMPDGFFTWLWDKLNV
                     FATVFLGIFVEAVPYLLLGTFGSGLVEVFVDRDQMSRWVSHRPAAAAISGAFMGMVFP
                     VCECGVVPLTRRLFKKGLPLSAGVSFLLAAPVLNPIVILSTASAFGWGQMLFWRFTIS
                     LVIAVVVGLVFSAQGDAAEVLRPVLAGEDHDHVHAEAGDSVGEKIRKALLITVDEFFD
                     MGRYLIIGSLLAAGLQTFIAQSSLLAIGSGPVLSV"
     gene            851..2584
                     /locus_tag="PLT08_05300"
     CDS             851..2584
                     /locus_tag="PLT08_05300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE03910.1"
                     /translation="MNYRHFKYEGWLYALAFLVALAVRFIALGALPLTDAEAAPALQA
                     LRISQSANPALDPHPFYILSTSLIFLMYGGGTNFLARLIPALIGSLLVLAPRLLDDRL
                     KPRPSLILAFFLALDPGLVAISRQITSPILAITFLAFTVGFINKNKWTWAAITAALAL
                     LSGPSAWLGLLGIGITYGIAKLLARRTEESSEEENTPTEKRKRDRSSSAFGKEFFKSS
                     NLITFALAFIFAGSLFFIVPGGIGAAFSSIPAFFKTWAATGPVTQGMLIISLFVYQPF
                     ALLLAGMALLRGWMGGLRRIVFLSIWFFVALLLVIFLSGRQMYDLAWALIPLNALAAL
                     ELVRHLSIFPEERREVAGVVLLTTFIWVFIWLGTAGFSWILVDTPDYRLRAGMLVGSI
                     FLLIISLVLVAAGWSIRIARLGGVWGMAICLGLLSVGGLFGATGLRGLSAPELWWLPN
                     VPTQAQLVRDTVHQVSDIGRGSIYSATVAIIGVNSPALEWAVRENPVQVVDAVDPNNT
                     PDFVITSYELDPELVSAYRGQDFTWRQAPIWNGADTFAWLKWISLRQMPQSQEAIILW
                     ARTDLFLGGKQ"
     gene            2651..>3087
                     /locus_tag="PLT08_05305"
     CDS             2651..>3087
                     /locus_tag="PLT08_05305"
                     /inference="COORDINATES: protein motif:HMM:NF014300.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004127 - cytidylate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HNE03911.1"
                     /translation="MTKTPPSIIALDGPAASGKSTVGRRLADALGYLFFDTGVMYRAV
                     TWIALNHDMDLKNEAAITQMAESAELDIRPPSQNDGRACDIIVGDKDITWDVRSGDVE
                     ASVSLVAAYAGVRTALSGQQRRIGLRGKVVMVGRDIGTVVLPEA"
     assembly_gap    3088..3187
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            3290..4000
                     /locus_tag="PLT08_05310"
     CDS             3290..4000
                     /locus_tag="PLT08_05310"
                     /inference="COORDINATES: protein motif:HMM:NF013702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HNE03912.1"
                     /translation="MSDIVQPPKPVTEIWKPELVRLPELTRLRLAFRKFAYGLVKLVV
                     WSCLRVTREGLENIPSSGPLLIVINHLGHADVPALISSLPYTPDALGEIELYDVPVLG
                     RLMDWYGVIWLHRGRPDKRALRAALDGLAEGRVIVIAPEGRYSLTGALEEGTGGAAFL
                     AHKSGAPILPIAVIDTENKSVFGHLKNWRHAPVTVRVGKVFRLVDQPSARKEALEMGT
                     SRIMAALADLLPEKYRGK"
     gene            4062..>4983
                     /locus_tag="PLT08_05315"
     CDS             4062..>4983
                     /locus_tag="PLT08_05315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE03913.1"
                     /translation="MDFILSLLAGIAFPAPPTLAGWLVWVSLLAVGATYLYRQRAAQS
                     AWGSKGWGLFLVLFLLVPILNLFIGLRLTSGSVRPLPGLPADAPGSALMVFSAIPWLL
                     GGGLLGPLAAAILGAFAGLVRGTWDLYSLFPVIEFAFLGVWFARNSRQRYRTPAYRLL
                     RQPLVGALLLIPIHTLFYTLSALFTQWGTEIPATARLDFAISNALVVTLAFSGEMLIG
                     GLVAQVISMTFPANWGSKQPLQPSPGEKSLESRFIFAVGSFILVLLLTLLIGGWVVAG
                     QAARGMLEDRLSSAGESAAQSVPFFLETGQN"
BASE COUNT         1008 a         1274 c         1324 g         1277 t
ORIGIN      
        1 agaccgaaag aaccggtcca cttccaatgg caagcaggga cgattgcgca atgaaagtct
       61 gcaagccagc ggcgagcagt gatccgatga tgagataacg acccatatcg aagaactcat
      121 ccacagtaat aagcagcgcc ttgcggattt tctcgccaac cgaatcgccc gcctccgcat
      181 ggacgtggtc atggtcttcg ccagccaaca ccggacgaag cacttctgcc gcatcacctt
      241 gtgccgagaa gaccaatccc accaccacag cgatcacaag gctgatggtg aatctccaga
      301 agagcatttg cccccaccca aatgcagagg cggtgctgag aatcacgatg gggttaagaa
      361 ccggtgccgc tagcaagaac gaaacaccgg cagaaagcgg aagccctttc ttaaagaggc
      421 ggcgggtaag tggcaccaca ccacattcac aaaccggaaa gaccatgccc atgaacgcac
      481 cgctgatcgc cgcggcggcg ggacggtgcg atacccaacg gctcatctgg tcgcggtcaa
      541 cgaatacttc taccaagcca gagccaaagg ttcctaacaa gagataaggc accgcttcca
      601 caaagatgcc aaggaatacc gtggcgaaga cgttgagctt gtcccatagc caagtgaaga
      661 agccatcggg catgaggctg gatgcaacag cgattagtac agccaaaagg atgaacaaaa
      721 tcgcattgcg cggtaatcga tttacagttg ttgtttgcac aggagagatt atactcaccg
      781 tcgcagcatc aaaattaaaa ccacttgagc gttctccttt atccacccca aacagattac
      841 aatagcgttt atgaattatc gtcatttcaa atacgaaggc tggttgtacg cacttgcatt
      901 cctggttgcc ctggcggttc ggttcatcgc cctcggcgcc ctgcccctta ccgacgcaga
      961 ggccgcgcct gctttgcaag ccttgcgcat ttcgcaaagc gccaaccccg ccctcgaccc
     1021 acacccgttt tacattcttt ccacttcttt aatcttcctc atgtatggcg gtgggacaaa
     1081 cttccttgcg cgactcatcc ccgctctgat tggaagtctg ctcgtgttgg cgccccgcct
     1141 tttggatgac cgcctcaaac cccgcccaag cctgatcctc gctttcttcc ttgccctcga
     1201 ccccggcttg gtcgccatct ctcgccaaat caccagcccc attctcgcca tcacattcct
     1261 cgcctttacc gtcggcttta tcaataaaaa taaatggacc tgggctgcta ttaccgccgc
     1321 ccttgccctc ctcagcggac cgtccgcttg gctgggtctg ttagggatcg gcatcactta
     1381 tggcattgcc aaactgctcg cccgccgcac agaagaatca tcagaggaag aaaacacacc
     1441 cacagaaaaa cgaaagcgag atcgttcgtc ttctgccttt gggaaagagt ttttcaaatc
     1501 ttctaacctt ataacttttg cacttgcctt tatctttgct ggctctctct tctttattgt
     1561 gcccggtggt attggcgctg cattttcttc catccctgct tttttcaaaa catgggcagc
     1621 gacgggtccc gttacacaag ggatgttgat catctctttg tttgtgtatc agccttttgc
     1681 gctgttgctg gcaggcatgg ctctactccg cgggtggatg ggtggtctgc gccgcatcgt
     1741 tttcttgagc atttggttct ttgttgcctt gttgctggtg atcttcctgt caggtcgcca
     1801 aatgtacgac ctcgcttggg cgctcattcc attgaatgcc ttggctgctt tggaattagt
     1861 acgtcacctt agcattttcc ccgaagagcg tcgcgaagta gcgggtgtgg ttttattaac
     1921 cacgttcatt tgggtattca tctggcttgg cactgcgggc tttagctgga tattggtcga
     1981 tacacccgat tatcgtttgc gggcaggcat gttggttggt tcgatcttct tgcttattat
     2041 cagtttggtc ttagtggcgg cgggttggtc cattcgcatt gcacggttgg gtggcgtgtg
     2101 gggcatggca atctgcttgg gtctgctgag tgtaggtggc ttatttggtg ctaccggctt
     2161 gcgcggatta agcgcccccg aactatggtg gcttcctaac gtcccaaccc aagcccaatt
     2221 ggtgcgagat acggttcatc aggtttcaga cattggcaga ggcagtatct attctgcgac
     2281 ggttgcgatc atcggcgtca actcaccggc gttggaatgg gctgtccgtg aaaatcccgt
     2341 gcaagtggtc gatgcggttg acccgaacaa tacgcctgat tttgtcatca cctcctatga
     2401 attagaccct gaactggtct cagcctatcg cggacaggac tttacctggc ggcaggctcc
     2461 catttggaat ggggcagaca cctttgcttg gttgaagtgg atttcgttgc ggcaaatgcc
     2521 tcaaagccaa gaagcgatca tcctctgggc acgaaccgac ctcttcctcg gcggaaaaca
     2581 gtaagcacca aagaccgtag aacttctacg gtcttttttt atagagtgtc tgatgtaaaa
     2641 tgggggcact atgacaaaaa ctcctccttc cattattgct ctcgacggac cagccgcctc
     2701 tggaaaatct acagtggggc ggcggctcgc agatgcattg ggttatttat tctttgatac
     2761 aggtgtgatg tatcgcgctg tgacgtggat cgcgctcaat cacgatatgg acctaaaaaa
     2821 cgaagctgcc attacccaaa tggcagagtc tgccgagctt gatattcgcc cgccctctca
     2881 aaacgacggt cgtgcctgcg atattatcgt gggcgataaa gacattacgt gggacgtccg
     2941 ttcgggagat gtggaggcta gtgtctcatt agtggcggca tatgcagggg tgcgcactgc
     3001 gctctctggt caacaacgcc gcattgggtt gcgtggtaag gtggtgatgg tcggcagaga
     3061 tattggcacg gtcgttttac ccgaagcnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3121 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3181 nnnnnnngtc gctccgctgc gccctgccga cgatgcgctg gtgttggata cagataaatt
     3241 atcggaagaa gaagtctttg ccaagatttt agagatgtgc aaataaagca tgtctgatat
     3301 tgtgcagccg cctaaacccg tcactgaaat ttggaagccg gaattagtgc ggctgcccga
     3361 gttgactcgt ctgcgccttg cctttcgcaa gtttgcgtat ggattggtta aactggttgt
     3421 atggagttgc ttgcgcgtca cacgggaagg gttggaaaat atcccgtcaa gcggaccttt
     3481 gctgatcgtt atcaatcatc tagggcatgc cgatgtgcct gcattgatct cgtccttgcc
     3541 ctatacgccc gatgcacttg gggaaattga gttgtacgac gtcccggtct tgggtcggct
     3601 catggattgg tatggtgtta tctggctgca ccgtgggcga cccgataagc gcgccctgcg
     3661 tgccgcgctc gatgggttgg cagaagggcg tgtcatcgtc attgcacccg aagggcgtta
     3721 ctcgctgacc ggcgcattgg aagaaggtac gggcggagcg gcttttttgg ctcataaatc
     3781 tggcgcaccc attttgccaa ttgcggtgat tgatacagag aataagagtg tttttgggca
     3841 tctcaaaaat tggcggcatg caccggtcac agtgcgggta gggaaagttt tcaggcttgt
     3901 cgaccagcct tcggctagga aagaggcgct ggaaatgggg acgagtcgca ttatggcagc
     3961 cctagcagac cttttgccgg aaaagtatcg cgggaaataa ttccaaaaat gtaaacaggc
     4021 ttgtgaacca cctgtggtat ttggggctat aattctaaaa aatggatttc atcctttcgc
     4081 tccttgcagg cattgcgttc cccgccccgc ccactcttgc aggatggctg gtatgggtgt
     4141 ctctgcttgc ggtgggggcg acgtacttat atcgtcaacg cgcggctcaa agtgcatggg
     4201 gctcaaaagg atgggggctt tttcttgtcc tgttcctgct tgttccgatc ttaaatttat
     4261 ttattggatt aagactgacc tccggctctg tgcgaccttt gcctgggttg cccgccgatg
     4321 cgccgggttc ggcgctgatg gttttttctg ccattccgtg gcttttgggt ggcggcttgt
     4381 tgggtccgct ggcagctgcg attctggggg cgttcgccgg tttggtgcgc ggcacttggg
     4441 acttgtactc gctcttcccg gtcattgagt ttgccttttt aggcgtttgg tttgctcgca
     4501 attcacgtca acgctatcgc acccccgctt atcgcctgct tcgtcagccg ttggtggggg
     4561 cgttgctttt aatcccgatt catacgttgt tctatacatt gagtgcgctt ttcactcaat
     4621 ggggcacaga gattcccgcc accgcacggc tagactttgc cattagcaat gccttggtcg
     4681 taacgcttgc ctttagcggc gagatgttga tcggtggttt ggtggcacaa gtcatttcga
     4741 tgactttccc cgctaattgg ggtagcaagc aaccgttgca gccttcgccc ggtgagaaga
     4801 gcctcgaatc tcgttttatc tttgcggtcg ggagtttcat ccttgttctg ttgctgactc
     4861 tactcatcgg tggatgggtg gtggcagggc aggcagctcg tggcatgttg gaagatcgtt
     4921 tgtccagcgc tggagagtcg gctgcgcaaa gtgtgccctt cttcttggag acggggcaaa
     4981 acc
//
LOCUS       DAOQJE010000149         4963 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_10008_length_4963_cov_6.515077, whole genome shotgun sequence.
ACCESSION   DAOQJE010000149 DAOQJE010000000
VERSION     DAOQJE010000149.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 4963)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4963)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4963
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10008_length_4963_cov_6.515077"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..995)
                     /locus_tag="PLT08_05320"
     CDS             complement(<1..995)
                     /locus_tag="PLT08_05320"
                     /inference="COORDINATES: protein motif:HMM:NF012360.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE03914.1"
                     /translation="MLKIILHAPNPIMPFCEPARELRIQNTPLWLHQRNLLAPYVTRE
                     MELKQGERLQPVREPAIVYRDNLFFDEAFITEFMTQALKLNRPVRAAFRADDPAFREH
                     ALPLSTSYTPAGSLYLADLWYYPNGPVAGAEPLVIDMQAKEIGYYHVPTYMADQSGDL
                     VFQVPLRCMIAIDSWVHIFIADMVFSQFARGARFEKRLNEDLMFKLKILGKAVYEGRQ
                     VLECSELVKVGKGCVIDPSAVIHGPTIIGDNVTINAGAVIENCVIGSNVNVSQDVQLM
                     LSVVGDGAFLPFRTALFMTTVMENSMVAQNTCLQMCVVGRNTFIGAGSTFTDYNL"
     gene            complement(1064..1675)
                     /locus_tag="PLT08_05325"
     CDS             complement(1064..1675)
                     /locus_tag="PLT08_05325"
                     /inference="COORDINATES: protein motif:HMM:NF014845.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-vinyl reductase"
                     /protein_id="HNE03915.1"
                     /translation="MSETYHYPQKMGRIVLLGMEEVIGKGGMDAVLNFAALNDLQPSA
                     TNERTFSFETVSKLHQSLEQVYGPRGGRGLALRSGRACFKYGIKEYGSLLGLTEMAFR
                     LLPLPTKLHTGARSFADLFNKHTDQQVRVEETESQILWHIERCPLCWERKTEEPVCHL
                     AVGLLQEALYWLSGGKVFSVEETACHARGDEHCIITIQKTPLT"
     gene            complement(1687..2469)
                     /locus_tag="PLT08_05330"
     CDS             complement(1687..2469)
                     /locus_tag="PLT08_05330"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HNE03916.1"
                     /translation="MSSITTDQGIVHYEVFGRGKPVILLHGYQGSWGLWQETMAYLGA
                     FYRTYALDFWGFGESGTKRSTYAVKDFVSLVYQFMEQLGIVRAPLVGHSMGGTVSLMT
                     AMQHPDRIQKVVVIGSPISGSSLYFFPRVFGYKTFGWLTYHNLWIYKGFYRILSPFYS
                     KDKNWAKMMDRDVSQVSLEAFFSSIGSLRRTDLRPSLHTIQAPVMGMYGDKDIVVNPN
                     QWKPLLDGVSKARIERFHTAGHFIMLDEPITFNQKLKSFLDE"
     gene            complement(2490..3026)
                     /locus_tag="PLT08_05335"
     CDS             complement(2490..3026)
                     /locus_tag="PLT08_05335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP/GTP-binding protein"
                     /protein_id="HNE03917.1"
                     /translation="MQTVKMVVTGPFSAGKTQFIQSVSEIDVVATERKISSDEERSVK
                     SATTVAMDFGRITVDQDLVLYLFGTPGQKRFDFMWEILSEGMLGFIVMVDSTRPETFR
                     EARSILETFRAYAPTPFVVAANKQDKEDAWDLEDMGHALRLDKNTKLLPCVATDRNSV
                     KTILLELLYSIMSEMEGK"
     gene            complement(3054..3860)
                     /locus_tag="PLT08_05340"
     CDS             complement(3054..3860)
                     /locus_tag="PLT08_05340"
                     /inference="COORDINATES: protein motif:HMM:NF025690.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4388 domain-containing protein"
                     /protein_id="HNE03918.1"
                     /translation="MALRGNLRDFTITQLLNLINLASKTGTLVIDGASEQAHIAFREG
                     KLAYARIGKEDGSLALVLYKANKINANQYKAIIDRAGQMSDKELGLLLINAGYVSRDD
                     ILLNLQAYFTDMVRRMFTWVEGVFRFEAEMLPPEDRINVRLDLENIIIEGSRQLRELE
                     QLQDEIPSLDMALKFTDRPLTNVNLSVDEWKVVKFVDPKNTMRQIAKTNKLTEVEVRR
                     IVYGLLQAGLVELVRPADAPTPQAAKPLAPQEVEDKKNLVNKLIGRIRSL"
     gene            complement(3988..>4963)
                     /locus_tag="PLT08_05345"
     CDS             complement(3988..>4963)
                     /locus_tag="PLT08_05345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNE03919.1"
                     /translation="FLPILLAQVIWGVGYTFTSGATQAWITDEVGEEKANPLFMRAAR
                     AGLVASLIGMGATMLIGANNAAMPIIVGAIGVILIGIVLIFIMPETGFKPTPMENRST
                     WQHMWHTFNEGVKSVKARPRLMNILGVGFFYGLYSEGFDRLWVKHLLDTFQIPVLFGG
                     NQVAFFSLLRFSAGLLTVFVMQFIEKRVDASSPLSIGRAMMIVTGVIALGIVGFALSS
                     LLPLTLGLYLTIYILRQVRIPLQTAWVNHKLDPQTRATVHSMTGQVDSIGQILGGGSA
                     FIARYLSVVAAITTSGILLGPALGLVRRANSQSTHEAEVDPVGELTGD"
BASE COUNT         1371 a         1325 c         1092 g         1175 t
ORIGIN      
        1 atcaagttgt aatctgtgaa tgtagagcct gcgccgataa acgtattacg tcccaccacg
       61 cacatttgca aacaagtatt ttgcgccacc atgctatttt ccatcacagt ggtcatgaat
      121 aaagctgtac ggaatgggag aaatgcacca tctcccacaa ccgaaagcat caactgcaca
      181 tcttgcgaga cattaacatt gctaccgata acacaatttt caataacagc ccccgcatta
      241 atggtgacat tatctccaat aatcgtcggt ccgtgaatca cagcgcttgg gtcaatgaca
      301 cagcccttcc ctacttttac caactctgaa cattccaaaa cctgacgacc ttcgtaaacg
      361 gctttaccaa gaatctttag tttgaacatc aggtcttcgt tcaatctctt ttcaaagcgc
      421 gcgccacgag caaattggct aaacaccatg tctgcaataa aaatatgcac ccatgaatca
      481 atggcgatca tacaacgcaa cggcacctga aaaaccaggt cgccactttg gtctgccatg
      541 taggttggta cgtggtaata cccaatttct ttcgcctgca tgtcaataac aagcggctct
      601 gcacccgcta caggaccatt cgggtaatac caaaggtccg caagatataa gctacccgcc
      661 ggggtatagg atgtagacaa aggcaaagca tgttcacgga aagccgggtc atctgcgcga
      721 aaagccgctc gaaccgggcg gttaagcttc aatgcctgcg tcatgaactc cgtaataaat
      781 gcctcatcga agaataagtt atcacggtaa acaatggcag gttcacgtac cggctggagc
      841 cgttcaccct gctttaactc catttcgcgg gtcacataag gggcgagcaa attccgctga
      901 tgaagccaaa gaggagtatt ttgaatccga agttcacgcg ccggttcgca aaaaggcatg
      961 atcgggttgg gcgcatgaag aataatctta agcatgggca gaattataaa tcatagcgtg
     1021 cctctcggca cgctatgtaa gaaaacaaaa ttgttatgca attttaggtt aagggggtct
     1081 tctgaatggt aataatgcag tgttcatccc cccgagcgtg acaagctgtc tcttccacgc
     1141 taaaaacctt gcccccactc agccagtaca acgcctcttg aagcaaaccc accgccaaat
     1201 gacaaaccgg ttcttcggtc tttctctccc agcacagcgg acagcgctca atatgccaaa
     1261 gaatctggga ctcagtttct tctacacgca cctgctggtc ggtatgttta ttgaaaaggt
     1321 ctgcaaaaga acgggcacct gtatgcagtt tggtcgggag cggcaataaa cggaacgcca
     1381 tttcagtcag cccaagtaga gagccgtatt ctttaatccc atacttgaaa caagcacgcc
     1441 ccgaacggag cgccaatcca cgcccaccac gcggaccata aacctgctcc agcgattgat
     1501 gcaacttgct caccgtttca aacgaaaacg tccgctcatt ggtagcggac ggctgtaagt
     1561 cattcaaggc tgcaaagttg agaacggcat ccatcccgcc tttgccgatc acttcctcca
     1621 taccgagaag gacgatcctt cccatctttt gggggtaatg ataggtttca ctcatgagtt
     1681 ttgtatttat tcatccagaa agctttttag tttttgattg aatgtaatcg gctcatccag
     1741 cataatgaag tgacctgctg tatgaaagcg ctcaatccgc gcttttgaaa caccatctaa
     1801 taagggcttc cactgatttg ggtttacgac aatgtctttg tcaccataca tccccatcac
     1861 aggggcttga atggtatgca agcttgggcg caagtccgta cggcgtaggg agccaatgct
     1921 tgaaaagaac gcttccaagg agacctgtga aacgtcacgg tccatcatct ttgcccagtt
     1981 tttatctttt gaatagaagg gagaaaggat tcgataaaaa cctttataga tccaaaggtt
     2041 atgataggtc agccaaccaa aggtcttgta accaaaaaca cgcggaaaga aatacagaga
     2101 agaccctgag atgggtgaac cgatcaccac tactttttga atccggtctg ggtgctgcat
     2161 tgcggtcatc aagctgaccg tcccgcccat gctgtgcccc accaatggcg cacggacgat
     2221 gcccaactgc tccataaatt ggtaaaccaa acttacaaag tcttttaccg cataggtgga
     2281 acgcttggta cctgattcgc caaaacccca aaaatcaaga gcataggtgc gataaaacgc
     2341 gcccaagtat gccatggttt cctgccataa cccccatgaa ccctgatacc catgcaaaag
     2401 gatgaccggt ttcccccgac cgaaaacctc gtaatgaacg attccctgat cagtcgtaat
     2461 tgaagacaca gtaagacatc acccgcccac tattttcctt ccatttcgct cataatgctg
     2521 tacaacagct caagcaaaat ggtcttaacg gagttgcggt ctgtcgcaac gcagggcagc
     2581 agtttcgtat ttttatcgag gcgcaaggca tgccccatat cttccaaatc ccaagcgtct
     2641 tccttatcct gcttatttgc agccacaacg aagggagtag gagcataagc gcggaaggtt
     2701 tcgagaatcg aacgcgcttc acggaaggtc tctgggcgtg tgctatccac catcacgatg
     2761 aatcccaaca taccttcaga aaggatctcc cacataaagt cgaaccgttt ctgaccgggg
     2821 gtgccgaaca ggtacaggac cagatcctgg tcaactgtga tgcgcccgaa atccatcgcc
     2881 acggttgtgg cagatttcac cgagcgttcc tcatctgagg aaatcttccg ttctgtggca
     2941 accacatcga tctcactgac agactgaata aactgggtct tgccagcgct gaatggacct
     3001 gtgactacca ttttgaccgt ttgcataatt cctatccttt cggcctgaaa aaattataac
     3061 gaacgaatgc ggccgatcag tttgttgacc aaattctttt tgtcttccac ttcttgcggg
     3121 gcgagcggct ttgcagcttg tggcgtaggt gcatcggcag ggcgaaccaa ttccacaaga
     3181 cctgcctgca ataaaccgta tacaatacgg cggacttcaa cttccgttaa cttgttcgtc
     3241 ttcgcgatct ggcgcatcgt attcttcggg tctacgaact tgaccacttt ccattcatcc
     3301 acgctcaagt tgacattggt caacgggcgg tcggtgaact tcagcgccat atccaagctt
     3361 ggaatttcat cctgcaactg ctccaattcg cgcagttgac gtgaaccttc gataataata
     3421 ttctcaaggt caagccgtac attgatgcgg tcttcagggg gcaacatttc cgcctcaaag
     3481 cggaacaccc cttccaccca tgtgaacatg cggcgcacca tgtctgtaaa ataagcctgc
     3541 aaatttagca gaatatcgtc acgagaaacg taccctgcat tgatcaacaa caaccccaac
     3601 tctttatcag acatctgccc ggcgcggtcg ataatggctt tgtactgatt ggcattgatc
     3661 ttatttgcct tataaagaac caaagcaagg ctgccatctt ccttgccaat acgggcatag
     3721 gctaacttac cctcgcggaa cgcaatatgt gcctgttccg acgcgccatc aatcaccaag
     3781 gttcccgttt tgctggcgag attgatgagg ttcaaaagtt gagtgatcgt aaaatctctt
     3841 aaattaccgc gcagagccat aattcctcaa attcccttca tagggagcgc tcgaaattat
     3901 acttggtaaa cagataaagc gtcaattaaa cctgtttgca ctcttgtatg ggtaggattt
     3961 atcaaatttc ccccgctatt ccgactttta atcgcctgtc aactccccta ctggatcaac
     4021 ctccgcctca tgcgtggact gtgagttggc tctgcgaacc aagccgagtg ctggcccaag
     4081 cagaatgcct gaagttgtaa tggctgccac taccgagaga tagcgcgcaa taaacgccga
     4141 cccgccgccc agaatttgtc caatcgaatc tacttgtccc gtcatcgagt ggacggtggc
     4201 acgagtctgc gggtcaagct tatgattgac ccaagcggtt tgcagaggaa tcctcacctg
     4261 acgcaaaatg taaatggtga gatacaagcc caacgtcaaa ggaagaagcg aggagagggc
     4321 aaatccgaca atgcccaagg caatgacgcc cgtcacaatc atcatcgctc tgccaatcga
     4381 aagcggacta ctcgcatcta cgcgtttttc gatgaactgc atcacgaaaa ccgtaagcaa
     4441 gcctgctgaa aatctcaaca gcgaaaagaa agcaacttga ttaccgccaa acaggactgg
     4501 gatttgaaag gtatcgagca agtgcttcac ccaaagacgg tcgaagcctt cactatacaa
     4561 gccatagaaa aagcccacgc ctaaaatatt catcaaacgc gggcgcgcct tcacagactt
     4621 cacaccttca ttgaaggtat gccacatgtg ttgccatgtg ctgcgattct ccatgggagt
     4681 tggtttgaag cccgtttcag gcatgatgaa gatcaaaaca atgccaatca gaatcacacc
     4741 aatggcgcca acgataattg gcatagctgc attgtttgca ccgataagca ttgtggcgcc
     4801 catcccgatc aacgaagcga ccaagcccgc tcgtgccgcc cgcataaaaa gcggattggc
     4861 tttctcctcc cccacttcgt cggtaatcca agcttgtgtg gcgccgctgg tgaaggtata
     4921 gcccacgccc caaatcactt gtgcaagcaa aatcggaaga aaa
//
LOCUS       DAOQJE010000150         4932 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_10093_length_4932_cov_4.359852, whole genome shotgun sequence.
ACCESSION   DAOQJE010000150 DAOQJE010000000
VERSION     DAOQJE010000150.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 4932)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4932)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4932
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10093_length_4932_cov_4.359852"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..488
                     /locus_tag="PLT08_05350"
     CDS             <1..488
                     /locus_tag="PLT08_05350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE03920.1"
                     /translation="ADIQLVYTNLMNASYNHLAAFNGTAGGNGQQGGNGNGNSGGNGQ
                     AGGAGQGQAGSGVPQANISGAVTVHGVVTSYDGMGISFTADDGQALYVDTGNMRYSQS
                     LGFAPVAGDGMTVVMFLGDQGTNSAISVTMDATGMVYTFRNETGQPLWAGGNGKGNGK
                     P"
     gene            903..995
                     /locus_tag="PLT08_05355"
     CDS             903..995
                     /locus_tag="PLT08_05355"
                     /inference="COORDINATES: protein motif:HMM:NF024731.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE03921.1"
                     /translation="MRQLNLSVRAYHRILKLARTIVDLAGSEEI"
     gene            complement(1059..1376)
                     /locus_tag="PLT08_05360"
     CDS             complement(1059..1376)
                     /locus_tag="PLT08_05360"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024898.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HNE03922.1"
                     /translation="MNSDGQWAAVALGPMAVLPAHQKQGVGSTLIRTGLKELLALGHN
                     VVIVLGHPEYYPRFGFKHSKPLGIQWEINVPEEVFMVAELSTGALSGRMGIVRYHSAF
                     KDV"
     gene            complement(1373..1573)
                     /locus_tag="PLT08_05365"
     CDS             complement(1373..1573)
                     /locus_tag="PLT08_05365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE03923.1"
                     /translation="MNLVIRPETPQDIAAIYQVNQLAFDRENEARLVDSLRHSGAVTL
                     SLVAQMDNEILSGTSYSRPLQL"
     gene            complement(1576..2142)
                     /locus_tag="PLT08_05370"
     CDS             complement(1576..2142)
                     /locus_tag="PLT08_05370"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013053.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase family protein"
                     /protein_id="HNE03924.1"
                     /translation="MEQISQNTALIIIDVQKGFDEPVWGERNNPQAEDNIVRLLEAWR
                     KYGRPVFHVQHMSLSPSSPLNPDHPGNAIKDELQPREGESLIQKNVNSAFIGTDLEQQ
                     LRARGCNSLVIVGLTTPHCVSTTTRMAGNFGFETYLVSDATAAFELTDHNGRTYSAQE
                     IHDVTLTTLNGEFAKVIETDMLLRLLED"
     gene            complement(2159..2797)
                     /locus_tag="PLT08_05375"
     CDS             complement(2159..2797)
                     /locus_tag="PLT08_05375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE03925.1"
                     /translation="MNKRNWLAFFGFLYVIVWVIGLLIPTGTLSPSMSNAELQQVLLA
                     NQLARLIQVYLIDGVAGIAILLFAVAAASLFRTVEEKPLARVVLSAGVVAGTISLVQA
                     AVQQTLVNPDLLASAETPFRTLLVLVNQIDTFKLMALALLSISTSMLGVRTRLIPAWV
                     NWLGIVLTLALILGGLSFAFANSLLTAVLFASLPMLLVWVGAVSAVAMRQVK"
     gene            complement(2797..3129)
                     /locus_tag="PLT08_05380"
     CDS             complement(2797..3129)
                     /locus_tag="PLT08_05380"
                     /inference="COORDINATES: protein motif:HMM:NF022279.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YrdB family protein"
                     /protein_id="HNE03926.1"
                     /translation="MFKMINLLVRFLLELCILAIFGYWGFQTGSNPFMKFLLSLGTPI
                     LFAVVWGTFLAPKSPMRLHEPWLFLLELVIFALTCWALYSTGKVDLTVAFGGIYILNK
                     ILMVIWRQ"
     gene            complement(3113..3367)
                     /locus_tag="PLT08_05385"
     CDS             complement(3113..3367)
                     /locus_tag="PLT08_05385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE03927.1"
                     /translation="MNANQRPIFYKIRLRGKLDLEQAAWFPELVHAEDDNGNTLLVGE
                     LPDQAALMGVLFRAHNLNLKILCVKIKTGKTGELKNVQND"
     gene            3511..4257
                     /locus_tag="PLT08_05390"
     CDS             3511..4257
                     /locus_tag="PLT08_05390"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014566.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HNE03928.1"
                     /translation="MKNLITKRPMIFSVIVMLILEAFVLAGLFFSRVTGVPLVSLDLP
                     MLMLNAVLAIVILSALRGWREAGFNAPSQWKKMSLFLVPLALLIGPTLFFQPQLPEPA
                     KIVVLIIVTLLIGFQEEAIFRGILLSAFRPQGVMKAVLISAALFGIIHINSLFVGRDP
                     IFVLSQIIASFLGAIGLGALRIRTNTVVPLVLLHAFNDFVQFSATGGMEAGDVALYIP
                     VLKVVISGLMALYGFYLLRGYKEETTSAAE"
     gene            4273..>4932
                     /locus_tag="PLT08_05395"
     CDS             4273..>4932
                     /locus_tag="PLT08_05395"
                     /inference="COORDINATES: protein motif:HMM:NF022441.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head GIN domain-containing protein"
                     /protein_id="HNE03929.1"
                     /translation="MMKQLLSALLVLTFATLACSTTIPGALVGSGDIVTESFDVSDFD
                     QIRLGTSGTLYVEQGDEFSLTIETDDNILPALNVDVTKGVLRLTADTDYTALQSTTLI
                     YRVTVPELTALDVSGSGEIFVEDLKSASLSMTVSGAGDTSFSNAEIDSLTLNVNGSGN
                     AIFENITAEDIIINVNGSGDATLAGKTEAVTITTSGSGKVFAEELETAKADVTINGSG
                     DT"
BASE COUNT         1167 a         1261 c         1342 g         1162 t
ORIGIN      
        1 atgccgatat tcaactggtg tacaccaacc tgatgaacgc ttcgtacaat cacctcgctg
       61 ctttcaatgg aactgcgggc ggtaacggac agcaaggcgg caatggaaac ggcaacagcg
      121 gaggaaatgg tcaggctggg ggagcaggtc agggtcaggc aggaagcgga gttccgcagg
      181 ctaatatctc cggcgcggtt actgtccacg gtgtggtcac atcctatgac ggtatgggta
      241 tcagtttcac agccgatgat gggcaggctc tctatgtgga cacgggcaac atgcgctaca
      301 gtcagtcgct tgggtttgcg cccgtggcag gtgatggtat gacagtggtc atgttcctcg
      361 gcgaccaggg cacgaacagc gcgatctcgg tcacgatgga tgcgacgggc atggtctaca
      421 ccttccgcaa tgaaaccggt cagccgttgt gggcgggcgg taatggaaaa ggcaatggaa
      481 agccgtaatc ttcaaacaaa tacgccccgc aagtgcgggg cgtatttgtt ttgatctttg
      541 tgaacacatt taggtaaaac caaccttgct gctactaaat actgtgttcc ctttagatta
      601 catcgccaat gcgatattgg ggtggctgat ggcggtggca gaggaaggtt tcatggcatt
      661 tttatacata aaatggctgg tttttagtcg aagatattgc cgttttacgc aaatttcctt
      721 tcggttgatg tattctcttg gaactacatg ataccttaga cgatgttttg gggcaattaa
      781 tttttgattg aagagtcatt aaatgttacc catatctact ccattgtgga tcaattccct
      841 gcaggaatgc cgctcatcaa acaacatcag agcttgatgc aggcggcgaa atcgtgctgc
      901 gcatgaggca actcaatttg tcggtgcgcg cctatcaccg catcctcaaa cttgcacgta
      961 caatcgtgga tctggcgggg agtgaagaga tatagtccgc gaacctagag gaagcactac
     1021 agtatcgtcc gaagttgaat ggggcgactt gattgcctct acacatcttt gaaggctgaa
     1081 tggtatcgaa caatacccat cctaccgctt agcgctcccg tacttaattc tgcaaccatg
     1141 aatacttctt ctggcacatt gatttcccac tgaataccaa ggggtttgga atgcttgaaa
     1201 ccaaaacgcg ggtaatactc gggatgcccc agaacaatga caacattatg cccaagagct
     1261 agaagttcct tcaacccggt gcggatcaat gtggagccga ccccttgttt ttgatgggcg
     1321 ggcaaaacag ccatgggacc caacgcgaca gcagcccatt gaccgtcgct attcacaact
     1381 gtaacgggcg agaataggat gtgcccgaca aaatctcgtt gtccatttgc gcgaccaatg
     1441 acagggttac tgctccacta tgacgaagag agtcaacgag gcgcgcttcg ttctcgcggt
     1501 cgaacgccag ttggttaacc tgataaatgg cggcaatatc ttgcggtgtt tcagggcgaa
     1561 tgacaaggtt catcgtcaat cctccaacaa cctaagcagc atgtctgttt ctataacttt
     1621 ggcgaactct ccgttcaggg ttgttagggt tacatcgtgg atctcttgtg cggaatatgt
     1681 ccgcccgttg tggtcggtga gttcgaaggc ggcggtggcg tctgagacga ggtaggtttc
     1741 aaagccaaaa ttgcctgcca tgcgcgtcgt cgtggagaca cagtgcgggg tggtgagacc
     1801 gacgatgacc agcgagttac atccgcgcgc acgaagttgc tgttccaaat ctgtgccgat
     1861 gaaggcgctg ttgacgtttt tctgaatcag cgattcacct tcacgtggtt gtaattcatc
     1921 tttgatggcg ttaccgggat gatctggatt caatggtgaa cttggagaga gggacatgtg
     1981 ttgaacatgg aagacagggc gtccgtattt acgccacgcc tctaacaacc tgacgatatt
     2041 gtcttcggct tgcggattgt tcctctcgcc ccagacaggt tcatcaaaac ctttttgaac
     2101 gtcgataatg atcagggcgg tgttttgaga aatttgttcc atatattgtc cttccacctt
     2161 attttacctg tctcattgca acagcgctga ccgcacccac ccacacgagc aacatgggca
     2221 gggaagcgaa caggacagca gtgagcagtg agttggcaaa ggcgaaactg agtccgccga
     2281 ggatgagggc gagggtgagg acgattccaa gccagttgac ccaggcaggg atgaggcgag
     2341 tgcgcacccc aagcatggat gtggaaatgc tcagcaacgc caacgccatc aatttgaagg
     2401 tgtcaatctg gttgaccaac accagcagcg tcctgaaggg agtctctgcc gaggcgagca
     2461 gatcagggtt gacaagggtt tgttgcacag ccgcctgcac gagtgagatg gtgcccgcga
     2521 ccacgcctgc gctcaagacc acacgggcaa ggggcttttc ctctacggtt cggaatagac
     2581 tggctgccgc cactgcgaag agcaggatgg ctatgcccgc taccccgtca ataagataga
     2641 cctgtatcaa acgcgccagt tgattggcaa gaaggacttg ctgaagttct gcattggaca
     2701 tggacgggga gagcgtcccc gtggggatca atagtccgat cacccaaacg atcacataca
     2761 gaaaaccgaa aaacgcgagc caatttcttt tgttcattac tgcctccaga taaccatcaa
     2821 aatcttgttg agaatgtaga taccgccgaa tgcgacggtc agatcgactt tgcctgtgct
     2881 gtataacgcc cagcacgtca gggcaaaaat gacgagttcg agcagaaaca gccagggttc
     2941 atgtaagcgc atgggcgatt tcggcgcgag gaaggtcccc cacactacgg cgaataggat
     3001 gggagttccc aagctgagca agaacttcat aaaggggtta ctgcctgttt ggaagcccca
     3061 gtatccgaag atggcgagaa tacagagttc aagtagaaag cgcacgagca gattaatcat
     3121 tttgaacatt tttgagttct cctgttttgc cagttttgat cttcacgcac aaaatcttca
     3181 aattgagatt gtgagcgcgg aagagaacac ccatcagcgc ggcttgatca ggcaactcgc
     3241 ccaccagcag cgtattgccg ttgtcgtctt cggcgtggac caactctggg aaccacgcag
     3301 cctgttcgag gtccagtttg ccgcgcaggc ggatcttata gaaaatcggg cgttggtttg
     3361 cgttcatgtc cccatcttat gcaggcgggt tgattctttc atctcccaat ggaggtattt
     3421 gtgggggtag tttctccccc gccgaaatcc ccctgttggg agatggaaaa ggaaagcaca
     3481 tcgcctacac tgggcggcaa ggagaaaaaa atgaaaaacc taattacaaa acgacccatg
     3541 atcttttctg tgatcgtcat gctgattctt gaggcatttg ttctggcggg gctcttcttt
     3601 agcagagtga cgggcgtgcc gctcgtctcg ctcgacctgc cgatgctgat gctcaatgcc
     3661 gtgcttgcca ttgtgatcct ttccgcgttg cgcgggtggc gggaggcggg cttcaatgcg
     3721 ccgtcccaat ggaagaaaat gagcctgttt ctcgtgccgt tggcgctgct gatcggaccg
     3781 accctctttt tccagccgca gctccccgag cctgccaaga tcgtcgtcct catcatcgtc
     3841 acgctgttga tcggctttca ggaagaagcc atatttcgcg gaatcctgct cagcgcattc
     3901 agaccgcaag gcgtgatgaa ggctgtcttg atctccgctg cattgttcgg aatcatccac
     3961 atcaattcgc ttttcgtcgg gcgcgatccc atcttcgtgc tgtcgcagat catcgcgtca
     4021 ttccttggag cgatcggctt gggcgcgttg cgcatccgca cgaacacggt cgtgccgctg
     4081 gttttgctcc acgcgttcaa tgacttcgtg caattcagcg caacaggcgg catggaagcc
     4141 ggagacgtgg ctctttacat ccccgttttg aaggtcgtca tttctgggct tatggcgttg
     4201 tacggtttct acttgttgcg cggttacaag gaagaaacca cctcagccgc tgaataaccc
     4261 aacaaggaaa acatgatgaa acaactactt tctgctttac tagtacttac gtttgcaaca
     4321 ctggcttgca gcaccaccat cccgggggcg ctggtcggct cgggcgatat cgtcactgaa
     4381 tcgttcgacg tgagcgactt cgatcaaatt cggctcggca cctctggcac gctgtatgtc
     4441 gaacagggcg atgaattcag cctgaccatc gaaaccgatg acaacatcct ccccgctttg
     4501 aatgtggatg tgaccaaggg cgtgctgcga ctgaccgcag acaccgatta caccgccctg
     4561 caatctacaa cgctgatcta ccgcgtgacc gtgcccgaat tgacggcgtt ggatgtctct
     4621 ggttcaggcg agatctttgt ggaggaccta aaatccgctt cgctttccat gactgtgagc
     4681 ggggcgggcg acacgagctt ttccaacgct gagatcgact cgctgacgct caacgtcaac
     4741 ggctccggca acgccatctt tgaaaacatc accgcagaag acatcatcat caatgtgaac
     4801 ggttcgggcg acgcgaccct ggctggcaag accgaagccg tcaccatcac cacgtccggc
     4861 tcgggcaagg tctttgctga agaactggaa accgccaaag cggatgtcac catcaacggc
     4921 agcggtgaca cc
//
LOCUS       DAOQJE010000151         4931 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_10096_length_4931_cov_7.206932, whole genome shotgun sequence.
ACCESSION   DAOQJE010000151 DAOQJE010000000
VERSION     DAOQJE010000151.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 4931)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4931)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4931
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10096_length_4931_cov_7.206932"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1555)
                     /locus_tag="PLT08_05400"
     CDS             complement(<1..1555)
                     /locus_tag="PLT08_05400"
                     /inference="COORDINATES: protein motif:HMM:NF028237.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase N-terminal 7TM
                     domain-containing protein"
                     /protein_id="HNE03930.1"
                     /translation="MYNADFLVPFVYILAAIFYALVGLYAWRKRPAVGVVSFAWVMLS
                     MSIWSLTYALEIFLPTINAKLQVLNIEYLGILGVPVFLFFFALDFTGRSHLLTPRFRL
                     ILWGVPAFILLMVWTNPLHSLMWGKVETFRSGSLSLLSVDFRSAFWVNIIYTYGLVIA
                     ACIILIMEFLQRPGSLRLQISFVILSLLASLLGSSTFVLNISPIVGVDLSPLFFLPAA
                     IGLAWVTLRYRLAEILSLEHITVLQNMKDSVIVLNETKKVIYINQVTEKLFNLTEDKV
                     IGQPFNFVSGRLANLLDPYISPNEQHHNEIEIGDGEEKKTYEVSISPIVSATRNTAEI
                     IIALHDITLRKEKEQELSRHGAIMSAVSRAAEQFLKTEDWEQNISNVLENLGQAADVS
                     RVSVKIEPVDNATYEWKAQENPYSEKPLSQVVFPVFVNGMQWATISFDEFRYDRQWSN
                     MELEAFQTAANILAAAETRTQTVHQLNRRQLALGLLQDIATVSLQAANLNEMAEVSAE
                     RLAKLIKADG"
     gene            complement(1677..2657)
                     /locus_tag="PLT08_05405"
     CDS             complement(1677..2657)
                     /locus_tag="PLT08_05405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE03931.1"
                     /translation="MPSVLETIANLFRPAPYPKDNTTEPAQIIRSKVLVIVYDPVMDA
                     AGTKLSKYMKWKDTTDLISGFMLDMLDVTEGKVRYEVVERIDVNEFPAKTDGFRYTAQ
                     TYLDVWNRTSAPHMPQEVNYSAILAQFNILNRVARSEIDEVWIFGFPYGGFYESTMAG
                     PGAFWCNAPPLANTALSKRRFVIMGFSYERGIGEMFESYGHRAESIMEKTFSKLTGEA
                     NLWKRFIRYDKVAPGKAACGNVHFAPNSQADYDWGNKTPVKSECYDWLLNFPNFKGDI
                     RTVDSYEWGGGEIRAHHKWWFNHFPRVAGRKNGIHNNWWQYIAAPQNVII"
     gene            complement(2676..>4931)
                     /locus_tag="PLT08_05410"
     CDS             complement(2676..>4931)
                     /locus_tag="PLT08_05410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE03932.1"
                     /translation="ALRAASDSLNKVLLDRNMSSSNRGQYTLAWLALAAVRESQSIFS
                     LCGPMHAYWFSANGTRHFFEPTISGKGLGSSQTINIHYAQSDLSPNDMLLFCGRLPNA
                     WVTPLEDAQPSSFDAMRRRLTSITNEDLNAVLIQTTTGAGVINLLRGNMVTKTEAPVE
                     VAPTETITEAPVQEQPQAIEEPNLEEEPAPQTSTPDLPATYEPESTPEGGAHLLQPST
                     YSLPPQREETAFPASIPNADPLANLPHRTGPREFPASIPRAKPRTEAEAPAIEENPIV
                     EKIEPKPEPVQEIKTEKESVPEIKIPEGPREPSPQAKQAAKIVVNLMQGFRTGSASLG
                     ERFRNFLPRLLPAEEQSTTSVSANAFMGFMAVLIPLIVVTLAVVVYLRYGRNEQYEAY
                     YNQALQMKQQALALTNPVEQRIAWENVLANLDQAENHNVTSDTMNLRQEAESKRDQLL
                     GISRMQFNPAFTAKLGIDVSRMAATENDLYLLNAANGEVLRAIPAGNGGFELDNTFNC
                     TPGVGNTVGPLVDILALPITNIYSATVLGIDASGNLLYCKPGATPQTGLLPAPDTNWG
                     RITSFILDSGNLYVLDAPSRAVWVYLGKESAFVDRPYFFFGQQTPTQDVIDFLVIGDE
                     MYLLHADGRISNCTYSRVDTNASKCQDPVNKINPFPGYADMDLFATAHFTQILFAAPP
                     DPSLLLLDADNQVVMRFAPRTLELQNQFHPTSGRSNPIPRSPVSAVAVSPDHVLYLAV
                     NGQVYFAPNMP"
BASE COUNT         1309 a         1071 c         1220 g         1331 t
ORIGIN      
        1 acccatctgc tttaattaac tttgctaggc gttctgctga tacttcagcc atctcattta
       61 agtttgctgc ctggagggat acggttgcga tatcttgtaa taaccccaaa gcaagttgac
      121 ggcgattgag ttgatgaaca gtttgtgtac gcgtctctgc agcggcaagg atatttgctg
      181 ctgtttggaa ggcttccaat tccatattgc tccattgacg gtcatatcga aactcatcaa
      241 aggaaatagt tgcccattgc attccattga caaagactgg aaaaaccact tgcgataaag
      301 gcttttcaga gtatggattt tcttgtgctt tccattcata cgttgcatta tcaacaggct
      361 caatcttgac agatacccgg ctgacatctg ctgcttgccc taaattttct agcacgtttg
      421 atatattttg ttcccaatct tcagttttga gaaattgttc agccgctcta ctgactgcag
      481 acataattgc cccatgacga ctaagttctt gctctttttc tttacgaaga gttatgtcgt
      541 gtaatgcgat tatgatttca gctgtatttc gtgtcgctga aacgattgga gaaatagaaa
      601 cttcataggt ttttttctct tcaccatctc caatttcaat ctcattatga tgctgttcgt
      661 tgggactgat atagggatct aataaattag ctaatcttcc cgatacgaaa ttgaagggct
      721 gcccaatgac tttgtcttca gtaagattga ataacttttc tgtgacttgg ttgatataga
      781 taactttttt tgtctcattt agaacaataa cgctgtcctt catattttga agaacagtga
      841 tgtgttcaag tgagaggatc tcagcgagac gatatcgtaa agttacccag gcaagcccaa
      901 tggcagcagg cagaaagaat agcggtgaaa gatctacgcc aacaatagga ctgatattta
      961 gaacgaacgt cgaagagccc aaaagcgaag caaggaggct taagattaca aaactgatct
     1021 gaagccttaa gctgcctgga cgctgcaaaa attccataat gagaatgatg caggctgcaa
     1081 taacaagccc atatgtgtaa ataatattta cccagaatgc acttctaaag tccacgctta
     1141 ataaacttaa ggagccagaa cgaaaggttt cgaccttgcc ccacattaaa gagtggagtg
     1201 gatttgtcca caccataaga agtataaaag cgggaacacc ccatagaatc agcctaaaac
     1261 gtggcgtaag taggtggctt ctgcctgtga aatctaatgc gaaaaaaaat aagaaaactg
     1321 gaaccccaag gattccgaga tattcaatat ttaatacttg taatttggca ttgattgtag
     1381 gaaggaagat ttccagcgca taggtaagcg accagattga catggagagc atgacccatg
     1441 caaatgagac aacacctact gccgggcgtt tcctccatgc atatagccct acgagcgcat
     1501 agaagatggc agcaaggatg taaacaaacg ggactaaaaa atcagcgtta tacattccct
     1561 tacaaatcta ttgtaatttt agcactgact aaagttcata ataataagat gatagtttgg
     1621 gtagggtttt tatctataca aaatggcact tcgttccaga acgaagtgcc attttgttat
     1681 atgatcacat tttgtggagc agcaatgtat tgccaccagt tattgtgaat gccattcttt
     1741 ctaccagcca ctcgtggaaa gtggttaaac caccatttgt ggtgagcgcg gatttcaccg
     1801 ccgccccatt cataagagtc cacagtgcgg atatcccctt tgaaatttgg gaagtttaat
     1861 aaccagtcgt aacattcact tttgacggga gtcttgttgc cccagtcata atctgcctgg
     1921 ctattgggtg caaagtggac gtttccgcag gcggctttgc ccggcgctac cttgtcgtag
     1981 cggatgaagc gcttccatag atttgcttcg ccggtaagtt tgctgaaggt cttttccatg
     2041 atcgattcgg cacgatgacc atatgactcg aacatttcgc caatgccacg ttcgtacgag
     2101 aatcccatga ttacgaagcg acgtttgctc aacgctgtat ttgctaatgg tggcgcattg
     2161 caccaaaaag cgccgggacc tgccatggta gattcataga acccaccata tgggaaaccg
     2221 aagatccata cttcgtctat ttcactgcgc gcgactcggt ttaagatatt gaattgggcg
     2281 agaatcgcgg agtaattcac ttcctgcggc atatgaggtg cagaggtgcg gttccagaca
     2341 tcgaggtagg tttgagccgt ataccggaag ccgtctgttt tcgcgggaaa ttcgttaacg
     2401 tctatgcgtt caacaacctc ataacggact ttgccctcgg tgacatccaa catatccagc
     2461 atgaagcctg aaatcaggtc tgtggtgtct ttccacttca tatacttgga aagttttgtg
     2521 cctgccgcat ccatgaccgg gtcataaacg ataaccaata ctttgctgcg gatgatttgc
     2581 gcaggctcgg tcgtgttatc tttcgggtag ggagcgggac gaaagagatt ggctattgtt
     2641 tcgagaacgc taggcatgga aacctcctta caacgttatg gcatgttggg ggcgaaataa
     2701 acctgaccgt tgactgcaag atacaacaca tgatcagggc taaccgctac ggcgctgaca
     2761 gggctgcgcg gaatagggtt cgatcttcca gatgtagggt ggaattgatt ttgaagttca
     2821 agcgtgcgcg gtgcaaaacg catcacaact tggttatctg catctaataa taacaaggat
     2881 ggatctggcg gcgctgcaaa caagatctga gtaaagtgtg ctgttgcgaa caagtccata
     2941 tcagcatacc cagggaaggg gttgatctta ttaaccgggt cctgacattt cgaagcgttc
     3001 gtatcaacgc ggctatatgt acaatttgaa atgcgaccat cggcatgcag gagatacatc
     3061 tcatcaccaa tgacgaggaa atcgatcaca tcttgtgtag gggtttgttg accgaagaag
     3121 aagtaggggc ggtcaacaaa tgcggattct tttccaagat acacccatac tgcacgcgaa
     3181 ggcgcatcga gcacatacag atttccagag tccaagatga aactggtaat gcgtccccag
     3241 ttagtatcgg gggcgggtaa gagaccggtt tgcggagtgg cgcccggttt gcaatataaa
     3301 agattgccgc tcgcatcaat acccaagacg gttgcgctgt aaatattggt gatgggcaaa
     3361 gcaagaatat ccactaatgg acctactgta ttgccaacac cgggagtaca gttgaacgta
     3421 ttgtctaact caaagccacc attcccagca gggatggcac gcagcacttc accatttgca
     3481 gcattgagca gatagaggtc attttctgtg gctgccatgc ggctgacatc aattccaagt
     3541 tttgcagtga acgccgggtt gaattgcatg cgagaaatgc ctagcaactg gtcgcgtttg
     3601 gattcagctt cctgacgaag attcatcgtg tcgctggtga cgttgtgatt ctcagcctga
     3661 tccaaattag caagcacgtt ttcccaggca atgcgttgtt caactgggtt agtgagagcc
     3721 agagcttgtt gcttcatttg taacgcttgg ttgtaatagg cttcatactg ttcattgcgt
     3781 ccataacgca ggtaaacaac cacggcaagc gttacaacaa tgagcggaat gagcactgcc
     3841 atgaatccca taaaggcgtt agctgaaacc gaagtggtgc tttgttcttc cgcgggtaaa
     3901 aggcgcggca aaaaattgcg gaagcgttca ccgagtgaag cgctgccggt gcgaaatccc
     3961 tgcatgaggt tgacgacgat cttcgctgct tgtttggctt gtggcgaagg ttcacgcgga
     4021 ccttcaggaa tttttatttc aggaacggat tctttttcgg ttttgatttc ttgaacaggt
     4081 tctggcttcg gctcgatttt ttcaacgata ggattctctt cgatcgccgg agcttctgct
     4141 tctgttcgag gtttggctct tggaatggat gccggaaatt cgcgtggacc tgttcggtgt
     4201 ggaagattcg ctaatgggtc tgcatttgga atagatgcgg ggaatgccgt ctcttcacgt
     4261 tgaggcggaa gagaataggt ggaaggttgt aagagatgag cgccaccttc aggcgttgac
     4321 tcaggttcat aggtggctgg caagtctggc gtcgaagttt gaggcgcggg ttcttcctcc
     4381 aaatttggtt cttctattgc ttgaggttgc tcttgaacag gcgcttctgt aatggtttct
     4441 gtgggggcga cttcaacagg tgcctctgtt tttgtcacca tgttgccgcg cagaaggttg
     4501 atgacgcctg caccagttgt ggtttgaata agcaccgcat tcaggtcttc attggtaatc
     4561 gaagtgaggc ggcggcgcat ggcgtcgaaa gaagagggtt gcgcatcctc aagcggagtc
     4621 acccaagcgt tggggagacg cccgcaaaaa agcaacatat cgtttgggct taggtcactt
     4681 tgtgcgtaat gaatgttgat agtttggctg gagcccaaac ctttgccaga aattgtaggt
     4741 tcaaagaaat gacgcgtgcc atttgcgcta aaccaatacg catgcatggg accacacaac
     4801 gaaaagatgc tctgactttc tcgcacagcc gcgagagcca accatgccaa cgtgtattga
     4861 ccacgattgc tagagctcat attacggtcg agcaacactt tattcaacga atcagaagcg
     4921 gcgcgcaaag c
//
LOCUS       DAOQJE010000152         4926 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_10116_length_4926_cov_5.035311, whole genome shotgun sequence.
ACCESSION   DAOQJE010000152 DAOQJE010000000
VERSION     DAOQJE010000152.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 4926)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4926)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4926
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10116_length_4926_cov_5.035311"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(5..775)
                     /gene="rfbF"
                     /locus_tag="PLT08_05415"
     CDS             complement(5..775)
                     /gene="rfbF"
                     /locus_tag="PLT08_05415"
                     /EC_number="2.7.7.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018040698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047343 - glucose-1-phosphate
                     cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate cytidylyltransferase"
                     /protein_id="HNE03933.1"
                     /translation="MKVAILAGGLGTRLSEETILKPKPMVELGGRPILWHIMKIYASY
                     GFKEFVVALGYKGELIKDYFLNYHLRSRSLTVQLRTGEVTTHSMDGEDWTVHLLDTGA
                     ETNTGGRVKRLSKFIGNETFMMTYGDGVSNVNIPALLEFHKKQAKLVTMTSVRPPARF
                     GQMVIEDNLVTQFKEKPQLGEGWINGGFFVIEPEACKYIPGDQTAWEFESLEHIAADR
                     QLAAYQHEDFWQCMDTLRDVHNLEKLWQEGNAPWKLWS"
     gene            complement(923..2314)
                     /locus_tag="PLT08_05420"
     CDS             complement(923..2314)
                     /locus_tag="PLT08_05420"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNE03934.1"
                     /translation="MVEINRIDGSFRDPSGFLFKHDQVLYRQVNKRYADEYTLLMSSG
                     LYESLIKSRLLVAHEEVNDIQPAEPKDAFKIIRPQRVNFISYPYEWSFSQLKDAALAT
                     LSIQKRAMKYGMSLKDASAYNIQFHEGKATLIDTLSFEKYTEGTPWVAYKQFCQHFLA
                     PLALMAKTDIRLSQLLRIHIDGIPLDLASGLLPFSTRLNFGLLTHIHLHARAQQQYAD
                     KQVETSTAQKGGMSKQALQGLVESLESAVKALTWKPVGTEWGDYYNFTNYSDSSFEQK
                     KEIIGKWVEQVKPRSVWDMGANTGFFTRIASEREIPSVAFDIDPAAVEKNYRQVKTSK
                     EKNILPLIQDLTNPSPSIGWNNRERDALSGRAPADMIFALAIIHHLAISNNVPLADIA
                     EFFHGLGKWLVIEFVPKSDSQVKKLLASRLDIFDEYTAEGFERYFIEKFCLKEKSQIL
                     GTERFLYLLETRP"
     gene            complement(2301..2501)
                     /locus_tag="PLT08_05425"
     CDS             complement(2301..2501)
                     /locus_tag="PLT08_05425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE03935.1"
                     /translation="MFFSHIALSPVFYLDPGSGSILFQMIVAALLGAGILIRSQWSRI
                     KRWFGVKPEVDENEDNEDNGGN"
     gene            complement(2658..3617)
                     /locus_tag="PLT08_05430"
     CDS             complement(2658..3617)
                     /locus_tag="PLT08_05430"
                     /inference="COORDINATES: protein motif:HMM:NF013761.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline dehydrogenase family protein"
                     /protein_id="HNE03936.1"
                     /translation="MLRSFFIYLSKAAWAQKLITGWSFSWRIASRFVAGVTLTDAIKI
                     VRELNAKGINATLDHLGEHTNTPEEAQQAADSIFATLDAIGADPSLRSNISIKLTQIG
                     MGLDESLCAETLERILARAKTAKTFVRVDMEDTPYTDKTINLYYAMREKGYENHGMVI
                     QAYLFRTEADVRRMLQDGVRIRNVKGAYNEPPDKAFPQKADVDANYDLITKILLDASL
                     AQKSKLSDDGRVPPIPAIATHDERRIAFAKSYAEKIGLPKNGMEFQMLYGIRRDLQES
                     LVKEGYLVRVYVPFGTHWYPYFMRRLAERPANVWFFIVNFFKG"
     gene            3743..>4926
                     /locus_tag="PLT08_05435"
     CDS             3743..>4926
                     /locus_tag="PLT08_05435"
                     /inference="COORDINATES: protein motif:HMM:NF024748.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HNE03937.1"
                     /translation="MRNRNTNTILRGVSILFLLGAFTLTVVSLISYSRQRTGYPAGMM
                     IAGVPVDGLSPAEASQRLLQVYNSPIEVNYNGAFFHIDPGAVGFTMDVETMLAAADLT
                     RTGGSFWGEFWDYLWNRAPAESEIPLSASISEDRLREFLQNEIASRYDIPPAPAQPVP
                     GGTTFLPGAPGQTLDVNRAVVIITNALRSPADRSVVLTFNQTTASRPTIENLEILLKQ
                     IITTSDFDGLIGYYMMDLQTGQEIHFLINNKQEITVEPDVAFTASSTIKLPILTSYLI
                     NHGTNLDTATTEAITRVFRISDNSATDSILERIDPNNGPIIVSQNMEQLGLTSTYISG
                     MFFLGAPNLIPGKVTPGNSRADIFTDPDPYSQTTPSEMGTLLADIYQCVQNGGGALIA
                     AFP"
BASE COUNT         1364 a         1232 c         1004 g         1326 t
ORIGIN      
        1 cggttcaact ccacagtttc catggagcat ttccttcctg ccagagtttt tcaagattat
       61 gaacatcccg cagtgtgtcc atgcactgcc agaaatcctc atgttgatac gccgcaagct
      121 gacgatctgc cgcgatgtgt tccagcgact cgaactccca ggcggtctga tcgccgggga
      181 tgtacttaca cgcctccggc tcgatgacga agaatccacc gttgatccag ccttcaccaa
      241 gctgcggctt ttctttaaac tgcgttacga ggttgtcttc gatcaccatt tgcccgaacc
      301 gcgcaggcgg gcggacggaa gtcatcgtca ccaacttagc ctgtttttta tgaaactcaa
      361 gcaaagcagg gatgttgaca ttgctcacgc catcaccata ggtcatcata aaagtttcat
      421 tgccaataaa cttagaaagc ctcttcactc gcccgcctgt gttggtctca gcgcctgtat
      481 ccaataaatg cacggtccag tcttcgccgt ccatgctatg tgtggtcacc tcgcccgtcc
      541 gcaattggac ggtcaagctg cgggaacgta gatggtaatt cagaaaataa tctttgatga
      601 gctcgccttt atagccaagc gcaaccacaa actccttaaa accgtaagaa gcgtatattt
      661 tcataatatg ccaaaggata gggcgcccac ctagttccac cattggctta ggtttaagga
      721 ttgtctcttc tgataagcga gtccctaatc cgcctgctaa tattgctact ttcatgaaaa
      781 tataattcct ttacaagtta agaataacca aattatacat gccagaaaaa attttacctc
      841 ttacaagatt gttattaaat acagcgaaag tccgttagaa aaccaacgga ctttcgctgt
      901 acacgtatta taaaaatttg tctcaaggtc ttgtttctaa aagatacaaa aacctctctg
      961 tacctaaaat ttgacttttt tcctttaggc agaatttctc aataaaatat cgttcaaatc
     1021 cctcagccgt gtactcatca aaaatatcca gccgggatgc gagtaatttc tttacttgcg
     1081 aatcagactt aggcacaaac tcgatcacca accattttcc cagcccgtga aagaattcag
     1141 cgatatccgc caaagggaca ttgttcgaaa tagcgaggtg gtgaataatt gccaaagcaa
     1201 aaatcatatc agcaggggcg cgaccagata aagcatccct ctcgcgattg ttccagccaa
     1261 tggatgggct cggattcgtt agatcttgta tcagcggaag aatgtttttt tctttagaag
     1321 tctttacttg ccgataattc ttttccacag ccgcggggtc aatgtcaaag gcaacagaag
     1381 ggatctctcg ttctgaagcg atgcgggtga aaaagcctgt attggcaccc atatcccaga
     1441 ccgaacgtgg cttgacctgt tccacccact ttccaataat ttctttcttt tgttcaaaag
     1501 aagaatcaga gtaattcgtg aaattgtagt aatcccccca ctctgtaccg acgggcttcc
     1561 aagtcaatgc tttaacagca ctttccaaac tttcaaccaa tccttgtaaa gcttgtttgc
     1621 tcatgccgcc cttttgagca gtggaagttt ccacctgctt gtccgcatat tgttgctgcg
     1681 ctctagcgtg caaatgaata tgtgtcaaca atccaaaatt caggcgtgtt gaaaaaggca
     1741 ggagtccact tgcaaggtca agaggaatgc cgtcaatatg aatgcgcaat aattgagata
     1801 atcgaatatc cgtttttgcc atcaaagcca gtggagcgag aaaatgctgg caaaattgtt
     1861 tataagcaac ccaaggtgta ccttcagtgt acttttcaaa ggaaagtgta tcaattaagg
     1921 ttgcttttcc ttcatggaat tgaatgttat aagcactcgc atccttaaga gacatgccat
     1981 atttcatggc acgtttttga atagacaagg tcgcaagtgc agcatctttt agttgcgaaa
     2041 aagaccattc atagggataa gaaataaaat tcactctctg cggacgaata atcttgaaag
     2101 catcttttgg ctctgctggt tgaatatcat ttacttcttc atgagcaaca agcaatctac
     2161 tcttgattaa gctctcatat aaaccagaac tcatcaaaag agtatattca tcagcatatc
     2221 tcttattgac ctgacgatac aaaacttgat cgtgcttaaa gagaaatccg cttgggtcgc
     2281 ggaaagagcc gtcaatacga ttaatttcca ccattgtcct cgttgtcttc gttttcatca
     2341 acttcaggtt tgacgccaaa ccaacgctta atgcgagacc actgagagcg tatcaaaata
     2401 cctgccccca aaagagcagc tacgatcatt tggaacaaga tgctgccaga tccagggtca
     2461 agataaaata caggagatag agcaatatga gagaaaaaca tgtttacttt ccttatggtg
     2521 aaataatgtc aagactgttt attttattgc agggacatca tggcgtcaat aaggttaatg
     2581 ttctgtaaaa gcgcaatcag cttttaaaca aaaaaagcgg tgaaaatctg tagattttca
     2641 ccgctttgat gagtctttta gcctttaaaa aagttgacaa taaagaacca cacattcgct
     2701 gggcgttctg ccaggcgtct cataaagtag gggtaccagt gcgtcccaaa cggaacataa
     2761 acacgaacca gatatccttc ttttacaaga gactcttgca aatctctgcg aataccgtac
     2821 aacatttgga attccattcc atttttaggc agccctattt tttcagcata gcttttggca
     2881 aaagcaatac gcctttcatc atgcgtcgca atcgccggga taggcggcac acgcccatcg
     2941 tcactgagct tcgatttttg tgccagcgaa gcatctaata aaatcttcgt gatcaggtcg
     3001 tagtttgcat caacatctgc tttttgagga aaggctttat caggcggctc gttatatgca
     3061 cctttaacat tgcgaatgcg gaccccgtct tgtaacatac ggcgaacatc cgcttcggtg
     3121 cggaagagat atgcttgaat aaccatgcca tgattttcat atcccttttc acgcatggca
     3181 taatacaagt tgattgtttt atccgtatac ggcgtatctt ccatatcaac gcggacaaag
     3241 gttttagcgg ttttagcacg agcgaggata cgctccaagg tttcagcaca caggctctcg
     3301 tccagcccca tgccgatttg tgtcagctta atggaaatat tgctgcgaag gctagggtcc
     3361 gctccgatag catccaatgt tgcgaagata ctatccgctg cctgctgcgc ctcttcaggg
     3421 gtattcgtat gctcccctaa gtgatccaaa gtagcattga tcccctttgc atttaactca
     3481 cggacaattt taattgcgtc cgtaagagta acccccgcaa caaaacgaga tgcgatccgc
     3541 caggaaaagc tccaacctgt gatcagcttt tgtgcccagg cggctttcga aaggtagata
     3601 aaaaatgaac ggagcatatc catcctttga actgtgattt agattaatat caacttattc
     3661 tagcacaagc cctatgtttc ggtatgttat actttttccg tgatttttgt catgcccatg
     3721 acatcggagg caatgtttaa ccgtgagaaa ccgaaatacc aataccatcc ttcggggtgt
     3781 ttcgatctta tttttactcg gggcgttcac actaaccgtc gtttccctta tctcttatag
     3841 ccgtcaaagg acgggctacc ccgctggcat gatgattgcc ggtgttccag tagatggctt
     3901 atcccctgcc gaagcatctc aaagacttct ccaagtgtac aactcgccaa ttgaagtaaa
     3961 ttacaacggc gcgttttttc acatcgaccc aggagcagtc ggcttcacca tggatgttga
     4021 gaccatgctg gcagccgcag atctgacaag aacaggcggc tctttctggg gcgaattttg
     4081 ggactacctc tggaaccgag cgccagccga atctgaaatc ccgttaagtg caagcatatc
     4141 cgaagaccgt ctgcgagaat ttctacagaa tgaaattgca tcgcggtacg atatccctcc
     4201 cgcccccgca caacccgtcc ccggcgggac aaccttcctc cccggcgctc ccgggcagac
     4261 tcttgatgtc aaccgtgctg ttgtgatcat cacaaacgct ttgcgctctc ctgcagaccg
     4321 ctctgttgta ttgacgttca accaaaccac cgcatcacgc ccaaccatcg aaaatcttga
     4381 aatcctgctc aaacaaatca tcaccacatc agactttgac ggtttgattg gttactacat
     4441 gatggacttg cagactgggc aggaaattca ctttctgatc aacaacaaac aggaaatcac
     4501 agtagagcca gacgtcgctt tcactgcatc cagtacgatc aagttgccca ttctgacttc
     4561 atacctgatc aatcatggca cgaatctcga tacagcgacc acagaagcca ttacccgtgt
     4621 gtttcgtatt tcagacaact ctgctacaga ttccatcctt gaacgtatcg atcccaataa
     4681 cggtccgatt atcgtctccc aaaacatgga acaactcggg ctgaccagta cctacatcag
     4741 cggtatgttc ttccttggtg caccaaactt gattccagga aaggtcaccc ctggcaattc
     4801 tcgcgcagac attttcacag acccagaccc ctattcgcaa acaactccgt ccgaaatggg
     4861 caccttgcta gcagacatct accagtgcgt ccaaaatgga ggcggggcgt taatcgcagc
     4921 cttccc
//
LOCUS       DAOQJE010000153         4925 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_10119_length_4925_cov_5.987269, whole genome shotgun sequence.
ACCESSION   DAOQJE010000153 DAOQJE010000000
VERSION     DAOQJE010000153.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 4925)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4925)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4925
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10119_length_4925_cov_5.987269"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            40..1404
                     /gene="der"
                     /locus_tag="PLT08_05440"
     CDS             40..1404
                     /gene="der"
                     /locus_tag="PLT08_05440"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016290913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HNE03938.1"
                     /translation="MKPIVALVGRPNVGKSTLFNRLVGERMAIVDDTPGTTRDRLFGD
                     AEWNARPFTVIDTGGIDPTHGGRTPLSVGSADFIGDIRRQAQAAIQEADAILFVVDGE
                     TGVTEPDREVADILRRMQKKQPDGSFLPPIILVVNKCESRERRDSVGEFYELGLGEPF
                     AISAIHGTNTGDLLDALVSSFPTQNEDEEDNNIKIALVGKPNAGKSSLLNKLVGEERV
                     IVSPIAGTTRDAIDMELEFGGLPITLIDTAGIRRRGKIEKGVEEYSVIRSFKAIERAD
                     VALLMIDATTGITSQDAHIAGFIKDEWKSCVVLVNKWDAIEKDGTTMDAYTEKILNDL
                     NFVSYVPILYISAKTGQRVDQVLPLALRVQEERLARLTTSKINEIIHAAQDAHPHPTH
                     AGRALKMYYGTQVRSDPPTFMIYVNEPSLMHFTYMRYLENQIRAEYGFLGTPIRIVLK
                     GRHE"
     gene            1397..1777
                     /locus_tag="PLT08_05445"
     CDS             1397..1777
                     /locus_tag="PLT08_05445"
                     /inference="COORDINATES: protein motif:HMM:NF022700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3054 family protein"
                     /protein_id="HNE03939.1"
                     /translation="MNKKNILIIGDVLAFAILNIIGFATHKETGVSFLPRMAVAFFPI
                     VFAWFVLAPWFGLFDETVINNSKNLLRIPLVFLFVAPLAVILRGALLNAPALPLFALV
                     FGSTNAFGMLVWRWLYLVLFAKKA"
     gene            complement(1793..3244)
                     /locus_tag="PLT08_05450"
     CDS             complement(1793..3244)
                     /locus_tag="PLT08_05450"
                     /inference="COORDINATES: protein motif:HMM:NF013736.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing phosphohydrolase"
                     /protein_id="HNE03940.1"
                     /translation="MVKTKFVEVSRSFIVIAILIWLAIAALGTIFLPGETGEIREMFA
                     FSQIIPILVAAFYFGQTGGLLAAFAASVVSGSLVIANIDNIDSIFVQRVMFQIISFNS
                     VALLTSFLSDQAKAHRRRLTRQVERLNALRAIDKAIISGGDLSNTLYILLESLTRLLN
                     VEAASIFLFDPKTEQLRLSASLGFFGEYPHQVLCNLGEGMVGQAAAEKHTIYHHNLEK
                     VDKQIIPAVKGEGVMDYYAVPLFANDRLRGVLEVFDRAPRLDSEWKSYLETLAGQAAI
                     AIAQSTLLGELKDANKELSEAYEETLKGWSRALNLRDKETDHHTQRVVELSVQLAGRM
                     GLRGEQLIQFERGAILHDIGKMGIPNEILLKPGKLTEEEWVIMRKHPLYAQMLIAPIN
                     YLEPAMTIPLYHHERWDGSGYPFGLHGTEIPLEARVFAIIDVWDALRSDRPYRKALLD
                     EEALTYIQAQADKQFDPEVVRVFAEMVRDNSLK"
     gene            complement(3238..3735)
                     /locus_tag="PLT08_05455"
     CDS             complement(3238..3735)
                     /locus_tag="PLT08_05455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HNE03941.1"
                     /translation="MQKDRSYIGLILFTLAYPAAHYLVGFIPNPFVPEANLAINMIFP
                     ILAGYFYGPISGAIAGFLGTGLSAILTADVYDALAILPHTMMGLVAGLAGNSRQQFLA
                     ACSVLLGHALNILFFSRFGYLLIERPPILILGLLTETTVDVVAVILFIVLLHRKLYRV
                     DDERW"
     gene            3857..4465
                     /locus_tag="PLT08_05460"
     CDS             3857..4465
                     /locus_tag="PLT08_05460"
                     /inference="COORDINATES: protein motif:HMM:NF019265.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HNE03942.1"
                     /translation="MDETALIASAQQGDLNAFNTLILHYQDAVFNTAVRILGDDDMAQ
                     DAAQDAFISAFQSIKSFRGGSFKAWILRTVTNACLDELRRQKRRPTTPLEPETHDGEE
                     MDSPKWLADSSMTPAQKAEADELEHAIQHCLDALPTEFRTAVVLADVQGMDYTEVATA
                     IRAPLGTIKSRIARARLRLRECLRSFEELLPSSFRLEEENAQ"
     gene            4462..>4925
                     /locus_tag="PLT08_05465"
     CDS             4462..>4925
                     /locus_tag="PLT08_05465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE03943.1"
                     /translation="MKNFYDVETLSAYLDGQLDASNAARLEARLKTDAELASALKDLR
                     AARGILKKLPARKAPRNFTLTRQMVGLKPPLPRTYPLLRFATAFAAVLFLFSFTANTL
                     VPMFNFGAAAPAYGLGSGGGPESAPMAAAATEAPATELFAAQEAAPASTLTT"
BASE COUNT         1345 a         1299 c         1053 g         1228 t
ORIGIN      
        1 cccacaccaa cccctaaacc ataaagtaaa ataaacccta tgaaacccat tgttgcacta
       61 gtcggccgac ccaatgtcgg taaatccacc ctgttcaatc gcctcgttgg cgaacgcatg
      121 gcaatcgttg acgatacccc cggcaccacc cgtgaccgcc tttttggtga tgcagagtgg
      181 aacgcccgtc ccttcactgt tattgataca ggcggtatag acccgactca cggcggtcgg
      241 actccgctct cagttggttc tgccgatttt atcggagaca tccgccgaca agcccaagct
      301 gccattcaag aagccgacgc catcctcttt gtcgtcgatg gcgaaaccgg cgtcactgag
      361 ccagaccgcg aagtggcaga tattttgcgc cgcatgcaaa agaaacagcc cgatggttct
      421 tttctgcctc cgatcatatt agtcgtaaac aaatgtgaat ctagagaacg ccgtgattct
      481 gtaggtgaat tttatgagct aggtttaggg gaaccctttg ccatttctgc cattcacggc
      541 accaacaccg gtgacttact cgatgcactc gtctcttcgt tcccgactca aaatgaagat
      601 gaagaagata acaatatcaa gatcgccttg gtgggcaaac ccaatgccgg gaaatcaagc
      661 ctgctcaata aactcgttgg cgaagaacgt gtgatcgtca gcccaattgc agggaccacc
      721 cgtgatgcca tcgacatgga attagaattt ggcgggttgc ccatcactct cattgatacc
      781 gcgggtatcc gtcgccgtgg caagattgaa aaaggcgtgg aagagtacag cgttatccgt
      841 tctttcaaag ccattgaacg cgcagacgtt gcgttactga tgatcgatgc caccaccggt
      901 atcacctcac aagatgccca tattgctggt tttatcaaag acgagtggaa atcttgtgtg
      961 gttttggtca acaaatggga tgccattgaa aaagatggca ccacgatgga tgcctacaca
     1021 gagaaaattc tgaacgacct gaattttgtt tcgtacgttc ccattcttta tatctctgcc
     1081 aagaccggtc agcgcgtgga ccaagtctta cccttggcat tgcgtgtcca agaagaacgc
     1141 ctcgcccgcc tgacgacttc gaagatcaat gaaatcattc acgccgcgca agatgcccat
     1201 ccgcacccta cgcatgccgg gcgtgctctt aagatgtatt acggcacaca agtccgctca
     1261 gatccgccca ccttcatgat ctatgtcaat gaaccttcgt tgatgcactt cacgtacatg
     1321 cgttatttag agaatcaaat ccgcgctgag tatggtttct taggaacccc cattcgtatt
     1381 gtcctcaagg gcagacatga ataagaaaaa catcctcatc ataggcgacg tcctcgcttt
     1441 tgcgattcta aatattattg gctttgcaac tcacaaagag actggcgttt ctttcctgcc
     1501 gcgtatggca gttgcctttt tcccaattgt ttttgcatgg tttgttctcg ccccatggtt
     1561 tggtttgttc gatgaaactg tcatcaataa ttcgaaaaat cttttgagaa tcccactggt
     1621 gtttctgttt gtagcccctc tggcagtgat cttgcgcgga gcattgctca acgcgccagc
     1681 gttgccgctt tttgcgttag tctttggcag tacgaatgcc tttggtatgt tggtgtggcg
     1741 ttggttatat ctcgttctgt ttgcgaaaaa agcgtaacat ctaaaattca catcatttca
     1801 aactattatc tctaaccatt tcagcaaaga cccggacaac ctccgggtcg aattgtttat
     1861 ccgcctgagc ttgaatataa gtcaacgcct cttcatctaa aagtgcttta cgatatgggc
     1921 gatccgaacg gagggcatcc cacacatcaa taattgcgaa cactctggct tcaaggggaa
     1981 tttctgttcc atgcaaccca aacggatatc cactcccatc ccagcgctca tggtgatata
     2041 gcggaattgt catggcgggt tcaagataat taatgggggc aattagcatt tgagcataaa
     2101 gcggatgctt tcgcatgatg acccattctt cttcagtcag cttccccggt ttgagcaaaa
     2161 tctcatttgg aattcccatc ttcccaatat catgcaagat cgcccctcgc tcaaactgga
     2221 ttaactgctc gcctcgcaat cccattcgtc ctgccagctg aaccgataac tccaccaccc
     2281 gttgggtatg gtgatccgtt tctttatcgc gcaaattcaa agcacgcgac caacccttca
     2341 aggtttcttc gtaggcttcc gataattctt tatttgcatc ttttagctcc cccagcaatg
     2401 tagactgtgc aatagcaatc gctgcctgcc cagccaaggt ttcaagatag cttttccatt
     2461 ccgaatccaa acgaggagca cggtcaaaaa cctccagtac tcctctcaaa cggtcatttg
     2521 caaacaaggg cacagcataa taatccatga ccccttcgcc ttttacagca gggattatct
     2581 gcttatctac tttttccaag ttgtgatgat aaatcgtatg tttttcagcc gctgcctgcc
     2641 ccaccatgcc ttcacccaga ttacacagca cttggtgcgg atattcgcca aaaaaaccaa
     2701 ggctagcgct taaacgtaat tgttctgttt tagggtcgaa caaaaatatg gaagcggctt
     2761 ctacattaag cagccgtgta agactttcca gtaatatgta aagagtattg cttaggtctc
     2821 ccccagaaat aatggcttta tcaatagctc gcaaggcatt gagacgctcc acctgccgcg
     2881 tcaaccttct gcgatgcgcc ttggcttgat cagacagaaa gctggttagc aaggctacag
     2941 aattgaacga tatgatctgg aacatcacac gttgaacaaa gatcgaatca atattgtcaa
     3001 tgttcgcgat tactaaagaa ccgcttacaa cgctggcagc aaatgccgct aaaagcccgc
     3061 ctgtctgacc aaaataaaaa gctgccacca aaatgggaat gatctgagaa aaggcaaaca
     3121 tctcccgaat ctcgcccgtc tcaccaggaa gaaagatcgt tcccaaggca gcaattgcca
     3181 gccatatcaa aatagcaatg acaataaaag atcttgaaac ttcaacgaat ttagttttca
     3241 ccatcgttca tcgtctacac gatatagctt tcgatgcaaa agtacaatga agagaatcac
     3301 agcgacaaca tccactgttg tttcagtaag caagcccaga atgagaatgg gaggtctttc
     3361 aataaggaga tagccaaaac gcgaaaagaa aaggatgttt agcgcatgtc ccaacaacac
     3421 agaacaagcc gccaagaatt gttgacgcga attccctgcc aaaccagcaa ccaaacccat
     3481 catggtatgt ggcaaaatcg cgagcgcatc atagacatca gcagtaagaa tggcagaaag
     3541 cccggtccct aaaaagcctg caattgcacc gcttatagga ccataaaaat accccgcaag
     3601 aatcggaaaa atcatattaa tagcaaggtt ggcttcaggg acaaacggat tggggataaa
     3661 ccccacaagg taatgagccg ctgggtatgc cagcgtgaat aagatcaatc ctatatagga
     3721 tctgtctttt tgcatctgca aattatagta gatagataca tctttttgta ttgcaaaata
     3781 taaaatctgt tatatatata caagaacact cccaaattat atagtgaaat gaaaagaccg
     3841 ggtaaaatta cagtctatgg atgaaactgc attaattgcc tctgcccaac aaggcgacct
     3901 gaacgcgttc aatacgctca tcctgcatta tcaggatgct gtcttcaaca cagctgtgcg
     3961 catccttggg gatgatgata tggcgcaaga tgctgcacaa gatgctttca tctcagcatt
     4021 ccaaagcatc aaatcatttc gcggcggctc gttcaaagct tggatcctgc gcactgtgac
     4081 gaatgcctgt ctcgatgaat tgcgccgcca gaaacgccgc cccacgactc cgcttgaacc
     4141 tgaaacccac gatggtgaag aaatggactc tcccaaatgg ctagcagatt cgagcatgac
     4201 accggcgcaa aaagcagaag ccgatgaact tgaacatgct attcaacatt gcctcgatgc
     4261 gttgcccact gaattccgca cagcagttgt gcttgccgat gtgcaaggca tggattacac
     4321 cgaagttgca accgcgatcc gtgcgccatt gggcaccatc aaaagcagaa ttgcacgcgc
     4381 acgattaagg ttacgtgagt gtttacgttc cttcgaggaa cttttgccct cctcatttcg
     4441 tctggaagag gagaacgctc aatgaaaaat ttttacgatg ttgaaaccct ctctgcatat
     4501 ttagatggac aactcgatgc ttcgaatgcg gcgcgactag aagcccgcct caaaaccgac
     4561 gccgagcttg cctctgcttt aaaagacctt cgcgctgctc gtggcattct caaaaagctg
     4621 ccagcacgca aagctcctcg caactttacc cttacccgcc aaatggtggg gctcaaacca
     4681 cctctgccac gtacctaccc attgcttagg tttgcaactg catttgcagc cgttctcttc
     4741 ctgttctcgt tcacggcaaa cacactcgtc ccaatgttca actttggtgc tgcagccccc
     4801 gcatatggat taggctctgg cggtggacct gaatctgctc cgatggcagc agccgctaca
     4861 gaagccccag ccactgagct atttgcagcc caagaagctg caccagcctc cactctcacc
     4921 accga
//
LOCUS       DAOQJE010000154         4867 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_10273_length_4867_cov_4.335204, whole genome shotgun sequence.
ACCESSION   DAOQJE010000154 DAOQJE010000000
VERSION     DAOQJE010000154.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 4867)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4867)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4867
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10273_length_4867_cov_4.335204"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..605
                     /locus_tag="PLT08_05470"
     CDS             <1..605
                     /locus_tag="PLT08_05470"
                     /inference="COORDINATES: protein motif:HMM:NF017946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC-2 family transporter protein"
                     /protein_id="HNE03944.1"
                     /translation="IALLGVFRLVNTMMIALIWPNTEKFNQSIRDGSMDYTILQPVNS
                     MFLVTFSRITVWRIWDLILAAVLIVVGVNMSAETTSALQILTFILLAISGMIVIYSLW
                     IVLIALTFWFVKFDNNVTILQALMDSGRYPATVYPAWLRIIVTFIVPIAVATTIPLQA
                     LRGELTVVQIFIFLGVSLASFVIASQVWKSGLKQYSGASS"
     gene            complement(638..1054)
                     /locus_tag="PLT08_05475"
     CDS             complement(638..1054)
                     /locus_tag="PLT08_05475"
                     /inference="COORDINATES: protein motif:HMM:NF015669.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="META domain-containing protein"
                     /protein_id="HNE03945.1"
                     /translation="MKLATLPALIFLALAMFVFNACSNTGTTLTGEWKLISYGDATNT
                     TPAIADSETSITFNEGKFGGTVGCNTFGADYTSEGSNIHIGSVVSTMMFCESTSAQES
                     AVLGILSDKTLKVSMNSNRLTLTTEDGASVVVLEKK"
     gene            complement(1147..>1945)
                     /locus_tag="PLT08_05480"
     CDS             complement(1147..>1945)
                     /locus_tag="PLT08_05480"
                     /inference="COORDINATES: protein motif:HMM:NF012797.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TrmH family RNA methyltransferase"
                     /protein_id="HNE03946.1"
                     /translation="NPFLVDSLNHPLAGPIRKLLTREGRRELNQILIDDEENILQAIE
                     AGVEIDSLYFAGEETVSETLRKNLTAGATIHEVAKRTTKKLFENDKISRIFAIAYTPQ
                     PVGLEALKSIKKDVVVLEDLSISGNIGNITRTSLALGVGGILLLNMDPVDLYDRRLIR
                     ASRGYLFSLPMITASTADFLDYCKKNNESLMVMDMHADKTVDDIAATPHRLLLAFGSE
                     KDGCSPEIENAATMKVRIPISDKVESLNVSASAGITLYSRSKFNQAN"
     assembly_gap    1946..2045
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(2141..2599)
                     /locus_tag="PLT08_05485"
     CDS             complement(2141..2599)
                     /locus_tag="PLT08_05485"
                     /inference="COORDINATES: protein motif:HMM:NF020022.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNE03947.1"
                     /translation="MSTLFLICGLPGAGKTTLAKELEESEPALRLCPDEWIQPILSDP
                     NNRQEMDRLRGHIEAIQWEFAKRSLSLGVHVILEFGFWSKQERANFRKEAEAIGANVR
                     LIYLDVEPEELWKRLEKRNANLTKGSFHVEKDELLDWVQLFEAPTPDEFA"
     gene            2652..3263
                     /locus_tag="PLT08_05490"
     CDS             2652..3263
                     /locus_tag="PLT08_05490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE03948.1"
                     /translation="MEYLLTSSPILGMLLWITLYISDYYMTLSSAKGYKEMSVMRFEN
                     SFELNPAFQKDIDSQTKVSSRHVTFLILYTAAILFMWFVAVKMFNAAWAYSFFIGMLV
                     LMEVTIHMRHFRNWYFLRVFRSKGGITGGITYAQWFSYQVSAFEFYCFAALYLLFFLL
                     TYSFFFLGGVMICLRIGVSHSNLAKKAERQHSASVAKNENSSS"
     gene            3244..3894
                     /locus_tag="PLT08_05495"
     CDS             3244..3894
                     /locus_tag="PLT08_05495"
                     /inference="COORDINATES: protein motif:HMM:TIGR00005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HNE03949.1"
                     /translation="MKILHRDSSIVVVDKPAELSVLAEGWSKDAPYLAKILEEQFGKI
                     WVVHRLDKGTSGVIVFALTAEAHRSLNIQFEKHEVEKAYHALVNGIPKWDEKVTKFPL
                     RVNVGHKHRTVVDDRNGVPAETRFRLLERYQAAALVEASPMTGRTHQIRVHAYALGYP
                     LVGDILYSAPENNLIARPALHARSLTLTHPENGKRLTFQADYPNDLLNAINLLRGK"
     gene            3937..4614
                     /locus_tag="PLT08_05500"
     CDS             3937..4614
                     /locus_tag="PLT08_05500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HNE03950.1"
                     /translation="MNERILIIEDDQAILKLLQRGLAYEGYTVDTATDGRMGLIAARD
                     HTPDLVVLDWMLPGMDGLEVCHRLRQGGSIPILMLTAKDTVQDRIQGLDAGADDYMVK
                     PFNLDELLARVRALLRRTQPERIPVLKFADLSLDTGSRQATRGNRTVALTAKEYELLE
                     LFLRHPKQVLTREVIFDRVWGYDFGGESNVLEVYIRYLRQKLEAENEQRLIHTVRGVG
                     YVLRENP"
BASE COUNT         1122 a         1084 c         1222 g         1339 t
ORIGIN      
        1 tcatcgcctt gctaggcgtc ttccgtcttg tcaacaccat gatgatcgcg ctgatttggc
       61 ccaacaccga aaaatttaac caatccatcc gcgatggctc catggactac accatcttgc
      121 agccagtcaa cagcatgttc ctcgtcacct tttcgcgcat caccgtctgg cggatctggg
      181 acttgatcct cgcggcagtc ttgatcgtgg tgggcgtgaa catgtctgcc gagacaacct
      241 ccgcgcttca gatcctgacc tttatcctgc tcgccatctc aggcatgatc gtcatctaca
      301 gtctttggat tgtcctcatc gcgctcacgt tctggttcgt caaattcgac aataacgtca
      361 ctatcctgca agccctcatg gactcgggac gttaccccgc caccgtctac ccggcatggc
      421 tgcgcatcat cgtcaccttc atcgttccca tcgcagtagc gacgaccatt ccactgcaag
      481 ccttgcgcgg cgagttgact gttgtgcaga tattcatttt cttaggggtg agtttggcaa
      541 gcttcgtgat tgcttcacaa gtttggaaat caggactaaa acaatatagt ggagctagta
      601 gttaaataaa aacggcgacc atttggtcgc cgttttttta tttcttttcc agcaccacaa
      661 cggatgcccc atcttctgtg gtgagtgtta accgattgct attcatagac accttcagcg
      721 ttttatccga aaggattccc agaacggcgc tttcctgtgc ggaagtcgat tcgcagaaca
      781 tcatagtcga aacgaccgag ccaatgtgaa tgttgctgcc ttcagaggta tagtccgcgc
      841 cgaaggtgtt acagcctaca gtgccgccaa acttcccttc gttgaatgtg atggaagttt
      901 ctgaatccgc aatggctggc gtggtatttg ttgcatctcc atacgaaatg agcttccatt
      961 cacccgtgag ggttgtgccc gtattcgagc aggcgttgaa cacgaacatg gcaagagcta
     1021 aaaagataag agcgggtaag gtggcgagtt tcataatatc ttctccttga tatatgttgt
     1081 tgacgccctc gcagtgaaaa agttcccgta aaaaaagaac cgaggttttc ctcggttcct
     1141 gttgggctaa ttagcttgat tgaattttga gcggctataa agtgtgatcc ctgccgaagc
     1201 cgaaacgttg agagactcca ctttgtcact gatggggatg cgcaccttca tcgtggcggc
     1261 gttttcgatc tctggcgaac acccatcttt ttcgctgcca aaggcaagca agagccggtg
     1321 gggagtggct gcaatatcat ccactgtttt gtcggcgtgc atgtccatga ccatgagcga
     1381 ctcgttattc tttttgcaat agtccaaaaa gtctgcagtg gatgcggtga tcatgggcag
     1441 ggagaagaga tacccccggc tcgcgcggat gagacggcgg tcgtacagat ccacggggtc
     1501 catgttgagc aggagaattc cgcctacgcc gagtgccagc gaagtacggg tgatattgcc
     1561 gatattgcca gagatgctca aatcttcgag cacaaccaca tcttttttga tggatttcaa
     1621 cgcctccaac ccgacaggtt gtggcgtgta ggcaatcgca aagatgcgcg aaattttgtc
     1681 attttcaaag agcttttttg tggtgcgttt ggctacttcg tgaatggtcg ccccggcagt
     1741 gagattcttg cgcagcgttt cagagacggt ctcttcgcct gcaaagtaaa gtgagtcgat
     1801 ctctactccg gcttcgatgg cttgcaggat attctcttca tcgtcaatga ggatctgatt
     1861 cagctcgcgc cgcccttcgc gggtgagcaa tttgcgaatg ggacccgcca gcgggtgatt
     1921 gagcgaatcg acaagaaaag ggttgnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1981 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2041 nnnnncccgc cagcgggtga ttgagcgaat cgacaagaaa agggttgttg gctgattgtt
     2101 cttccatatt ggtcatgata acgccctaac ggaatgagtg tcaagcaaat tcgtctggag
     2161 tgggggcttc aaagagttgt acccagtcca acagttcatc cttttcaaca tgaaagctgc
     2221 ctttggtaag gtttgcgttt cgtttttcaa gccttttcca tagctcctca ggctctacat
     2281 ctaagtaaat caggcggacg ttcgctccga tggcttcggc ttcttttcga aagttcgccc
     2341 gttcttgctt cgaccagaat ccgaactcaa gaataacgtg aacgcccaaa gagagagaac
     2401 gtttggcaaa ctcccattga atagcttcga tgtgaccgcg caatctatcc atttcttggc
     2461 ggttatttgg gtcagataag atgggttgaa tccattcgtc tgggcagagg cgtagggcag
     2521 gctctgattc ttccaattct ttagcgagag tcgtttttcc ggcgccgggt aacccacaaa
     2581 tcaggaataa agtagacatg cttatacatt gtaatagggg agcaggtaaa attagagtca
     2641 gaggagttga tatggaatac ctgctcacga gttcccccat tcttggcatg cttctgtgga
     2701 tcacgcttta catcagcgat tattacatga ccctttcttc tgcgaaggga tataaagaaa
     2761 tgagcgtgat gcgctttgag aatagctttg aattaaaccc ggcatttcaa aaagatattg
     2821 atagccaaac caaagtttca agtcgacacg tcacgttctt gatcttgtat acagccgcta
     2881 ttctttttat gtggtttgtt gctgtaaaaa tgttcaatgc ggcttgggca tactcttttt
     2941 ttatcggtat gcttgttctc atggaagtga ccatccatat gcgtcacttt cgtaattggt
     3001 attttttgag ggtatttcgt agcaaaggcg gaatcacagg tggtatcacg tatgctcagt
     3061 ggttttcata tcaggtatct gcttttgaat tctattgctt tgccgccttg tatttgctct
     3121 tcttcttact tacctacagc tttttctttt taggcggggt tatgatttgt ttacgtattg
     3181 gagtgtctca ttccaatctg gcaaagaaag ctgaacgcca acactctgcc tctgtagcta
     3241 aaaatgaaaa ttcttcatcg tgattcgtct attgtagtgg ttgataaacc agccgagctc
     3301 tctgtccttg cggaggggtg gagtaaggat gcaccatatc ttgcaaaaat tttggaagaa
     3361 caatttggga aaatttgggt ggtgcatcgt ttggataaag gcacaagcgg agtgatcgtc
     3421 tttgcactga cggcggaggc tcatcggtcg ctgaatattc agtttgagaa gcatgaagtg
     3481 gaaaaagcct atcatgcctt ggtgaacggt attccaaaat gggatgagaa ggtgacgaaa
     3541 tttccattgc gtgtgaatgt ggggcataaa catcgcactg tggtggatga tcgcaatggc
     3601 gtcccggctg agacgaggtt taggctgctg gaacgctatc aagctgcggc gttggtagag
     3661 gcgtcgccaa tgacggggag aacgcatcag attcgtgtcc atgcctatgc gttgggttat
     3721 ccgctggtgg gggatatcct ttatagcgca ccggaaaaca acttgatcgc gcgcccggca
     3781 ttgcatgcac gatcgttgac attaacacat cctgagaatg gtaagaggtt gacctttcaa
     3841 gcagactatc ctaatgattt attgaatgct ataaatttgc tgagaggcaa ataagcctct
     3901 tgaatttatg gtatattttt agtaaggagt atagccatga acgagcgaat cctcatcatc
     3961 gaagacgatc aagctatctt aaagttgtta cagcgcggtc tggcgtatga aggttatacg
     4021 gtggataccg ccacagatgg acgcatggga ttgatcgcgg ctcgcgacca tacccccgat
     4081 ctggtcgtcc tggattggat gttacccggc atggatggtt tggaagtgtg ccatcgtttg
     4141 cgacagggcg gttcaattcc gattttgatg ctgactgcca aagataccgt gcaagaccgc
     4201 attcagggat tggatgccgg tgcggatgat tacatggtca aaccgtttaa cctggatgag
     4261 ttattggcac gtgtgcgtgc gcttctgcgc cgaacccagc cagaacgtat tcctgttttg
     4321 aagtttgcag acttgtcact ggatacgggg tcacgtcagg caactcgcgg gaatcgcacc
     4381 gtcgctttga ccgcgaagga atacgaatta ctggaattat tcctgcgcca ccctaagcaa
     4441 gtgctcactc gtgaagtgat cttcgaccgt gtgtggggct acgattttgg cggcgagagc
     4501 aatgtgttgg aagtgtacat tcgttatctg cgccaaaagt tggaagccga aaacgaacaa
     4561 cgccttattc ataccgtgcg cggtgtgggg tatgtcttaa gggaaaatcc gtaatggaac
     4621 ggcgggtgac cgccgttttt ttattgtagt gagaaatatc agggtatgaa atgatttttc
     4681 tcaccatcta ttcttagctt gccctaaact cgaaccatta aactgtgacc atgtcgttgc
     4741 gtttgcgcct caccttgcta tactccacct ttatgggggg gattttgctt gtttttggcg
     4801 cggcggttta tattcttgta aacgtgattt tgctcaacca ggtggataac ttgttagcaa
     4861 acgtggc
//
LOCUS       DAOQJE010000155         4864 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_10286_length_4864_cov_5.000208, whole genome shotgun sequence.
ACCESSION   DAOQJE010000155 DAOQJE010000000
VERSION     DAOQJE010000155.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 4864)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4864)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4864
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10286_length_4864_cov_5.000208"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1574
                     /locus_tag="PLT08_05505"
     CDS             <1..1574
                     /locus_tag="PLT08_05505"
                     /EC_number="6.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013225.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="acyl-CoA carboxylase subunit beta"
                     /protein_id="HNE03951.1"
                     /translation="SPKGINMTQEDPRILELRRVRAKAKEGGGEERNAKQRAKGKMTA
                     RERIEALLDPGTFNEIEPFITHQGDELGLLKEKFYGDGVITGYGQINGRTVYLYSQDF
                     TIYGGTLSEMQSHKICRVMDLAVRNGVPFISLIDSGGARIQEGVRSMGGYAEIFRRNA
                     QYSGVVPQVSVMLGPCAGGAAYSPALTDLVIMVEKQSFMFLTGPDVIKAVTGEVIDAE
                     SLGGSVVHNSVSGVAHLSVTSEMAALEMARKFLSYLPSNNVENPPYVQPSDALSRMDE
                     DLNHIIPLEANEPYVMHQVIEKIVDKNSFLEIQPDWARNAIVGLARIGGHSVGIVSQE
                     PSIMAGVIDIDAADKMTRFVRMCDCFNIPLVTFVDSPGFLPGIAQEHGGIIRHGAKLL
                     YAYSEATVPKICIITRKAYGGAYVVMSSKYLGTDVTYAWPSAEIAVLGAEGAANILYK
                     KQIETAADPATERKRLADEYREKFNNPYYSASTGYVDDIIEPRESRPKIIASLSALRD
                     KYAPAPPRKHGNIPV"
     gene            1577..1906
                     /locus_tag="PLT08_05510"
     CDS             1577..1906
                     /locus_tag="PLT08_05510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE03952.1"
                     /translation="MSTFNLSLQITALGMGLVFGAILLLWLMMVVLTAITADKEPASD
                     SAKVDSTPLADSKLQVALIAVAMAMAEQEASSAHPLEQPPTTIVSAWQLGMRTSQMSE
                     KGNFRRH"
     gene            1908..2339
                     /locus_tag="PLT08_05515"
     CDS             1908..2339
                     /locus_tag="PLT08_05515"
                     /inference="COORDINATES: protein motif:HMM:NF012584.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoyl-binding protein"
                     /protein_id="HNE03953.1"
                     /translation="MKYKLNVNSQTYEVEIENINARPVVVTVDGQRFEVMPESSTQAQ
                     TKNEAVSNIEVKPTMPSPLGVANSSVSGSSSLHAPLPGTVVEVFVKAGDSVEAGQVIL
                     VIEAMKMKNSIRTTRSGTIANVLVNQGQSVAHKQTLVEFTS"
     gene            2387..3520
                     /locus_tag="PLT08_05520"
     CDS             2387..3520
                     /locus_tag="PLT08_05520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium ion-translocating decarboxylase subunit
                     beta"
                     /protein_id="HNE03954.1"
                     /translation="MDLNSLLPELLKGFSHFTLGNGVMIFAALVLIYLAVFKEIEPVL
                     LLPIGFGCLLANIPLAGMTAAEGMTKVIYDAGIKTELFPLLIFIGVGAMIDFSPLLAQ
                     PRMVLLGAAGQFGIFGTLMLAVALGFPLNQAASIGVIGAIDGPTSIFVATKLAPELLA
                     PIAVAAYSYMSLIPIIQPPLMKLFTTKKERLIQMEYTPTPISKTTLIFFPVVLTLVVG
                     LLVPEATPLISMLMLGNLLKVTGVVDRLSNAAQNEIINIATLFLGLAIGSTMNAAAFL
                     NLATGKIMLLGLFAFALDTVAGLLFGKFMAFITGGKINPLIGAAGISAFPMAGRLAAK
                     TANDEDNNNFILMHAMGANTAGQLGSVIAGGILLSVVSKILGI"
     gene            3547..3876
                     /locus_tag="PLT08_05525"
     CDS             3547..3876
                     /locus_tag="PLT08_05525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE03955.1"
                     /translation="MKLKKEAQMKLEGSFIFASLAFLFAIGSIIYLKRMQNVIENDIK
                     HRIRTEYPPESRKQILEFYRRLKIKEMEGLFSKILDDAKGDVNKVGKLTNVAESIGWK
                     AFLENEW"
     gene            complement(3916..4419)
                     /locus_tag="PLT08_05530"
     CDS             complement(3916..4419)
                     /locus_tag="PLT08_05530"
                     /inference="COORDINATES: protein motif:HMM:NF033634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4231 domain-containing protein"
                     /protein_id="HNE03956.1"
                     /translation="MTENEQLNPNEPIDDKYVLDKYNNKISYYWGASRSNKNSYKRYR
                     AWTVILGALVTLVSSISAAEFVQKPEWLRVAFAVATPVIAATLTVINGLGQNFHWGAT
                     WRDMVLNGTRLEKERDRFLATKPEDRDLVKELDRLNTIVLDETRNFFQRVLDSEVKPG
                     KDQNGNG"
     gene            4568..4855
                     /locus_tag="PLT08_05535"
     CDS             4568..4855
                     /locus_tag="PLT08_05535"
                     /inference="COORDINATES: protein motif:HMM:NF018717.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1330 domain-containing protein"
                     /protein_id="HNE03957.1"
                     /translation="MTAFVILDIEVTDPVGYEDYKKLAVPSLEPYGGKYAARGGKVEN
                     LEGDWSPTRIVVIQFESVERAKAWINSPEYSEARALRHKYAVSKAIVVEGL"
BASE COUNT         1293 a         1122 c         1161 g         1288 t
ORIGIN      
        1 tttcacctaa aggaattaac atgactcaag aagaccccag aattttggaa ttacggcgag
       61 ttcgtgcgaa ggcaaaggaa ggtggtggcg aagaacgcaa tgccaaacag cgtgccaaag
      121 ggaagatgac ggcacgcgaa cgcattgaag ccttgcttga cccaggcacc ttcaatgaga
      181 tcgaaccgtt catcacccac caaggcgatg agctgggatt actgaaagag aaattttacg
      241 gggatggtgt gatcacaggg tatgggcaaa ttaacgggcg gacggtttac ttatattcac
      301 aagattttac aatttatggc ggcacactca gcgagatgca atcgcacaaa atttgccgtg
      361 tgatggatct ggcagttcgt aatggcgtgc cattcatttc gttgatcgac tcaggtgggg
      421 cgcgcattca agaaggtgtg cgttcaatgg gcggatatgc tgaaatcttt cggcgtaatg
      481 ctcaatattc gggtgtggtg cctcaagtca gcgtgatgct tggaccctgt gctggcggtg
      541 cagcctactc acccgctctg accgacctgg tcattatggt ggaaaaacaa tcattcatgt
      601 tccttaccgg tccagatgtc atcaaagctg taactggtga agtgatcgat gctgagtcct
      661 tgggcggatc cgtggttcat aactcggtga gcggcgtggc acatttgagt gtcaccagtg
      721 aaatggcagc attggaaatg gcacgcaaat tcctttcgta tttgccgtcg aataacgtag
      781 aaaacccgcc gtatgttcaa cccagcgatg cactttctcg catggacgaa gaccttaatc
      841 acatcattcc attggaagcg aatgaaccct atgtgatgca ccaagttatc gagaagatcg
      901 ttgataaaaa ctctttcctt gaaattcaac ccgattgggc acgcaatgcc attgtcgggc
      961 tggcacgcat tggtggacat agtgtgggca tcgtttcgca agaaccgtcc attatggcag
     1021 gtgtgatcga tattgacgct gctgataaga tgacacgctt cgtccgcatg tgcgattgtt
     1081 tcaacattcc gctcgtaacc ttcgtggact caccgggctt cttacccggc attgcgcagg
     1141 agcatggcgg cattattcgt cacggtgcga aattgcttta tgcatactct gaagcgactg
     1201 tgccaaagat ttgtatcatc acccgcaaag cctatggcgg cgcctatgtc gtgatgtcta
     1261 gcaagtattt gggcaccgat gtcacctacg cctggccttc tgcagaaatc gccgtactcg
     1321 gcgcagaagg ggccgcaaat attctttata agaagcagat cgaaaccgcc gcagacccgg
     1381 ctactgaacg caaacgcctt gcagatgaat atcgcgagaa attcaacaac ccgtattact
     1441 ctgcatctac tggatacgtc gatgacatca ttgaaccgcg tgaaagccgc ccgaagatca
     1501 ttgcatcact ttcggcgctt cgcgataaat atgctcccgc ccccccacgc aagcacggga
     1561 acattccggt gtaatcatga gcacatttaa tctctctttg caaatcactg ccctcggcat
     1621 ggggcttgtc tttggtgcga tcctcttact atggctgatg atggtagtgc tcactgcaat
     1681 tactgcggat aaagaacccg cctcagactc agccaaagtt gactctactc ccttggcaga
     1741 ttccaagctg caagtggcgc tcattgcggt tgccatggca atggcagaac aagaggcttc
     1801 ttctgcacat ccgctagaac agccacccac taccattgtc tcagcatggc agctcggcat
     1861 gaggacaagc caaatgtcgg aaaaaggaaa ttttagaagg cattagcatg aaatataaac
     1921 taaacgtcaa cagccaaacc tatgaagtgg aaattgaaaa tattaacgcc cgtccggtcg
     1981 tggtgacggt agatgggcag cggtttgagg tcatgcctga gagtagcacg caagctcaaa
     2041 caaaaaatga agcggtttct aatattgaag tcaaacctac gatgccgagt ccgcttgggg
     2101 tggcaaactc atcagttagc gggagttcat ctctccatgc tccgctaccc ggcaccgtgg
     2161 tggaagtgtt cgtgaaagca ggcgactctg tggaagccgg gcaagtgata ttggtcattg
     2221 aagcaatgaa gatgaaaaac agcatccgca caacacgcag cggcacaata gccaatgtgc
     2281 ttgtaaacca gggtcaaagt gtggctcaca aacaaaccct ggttgaattc acatcttaat
     2341 tttacgaatg acttaatagg cattgctgca ttcgtaaagg actcttatgg acttaaattc
     2401 tttgcttcct gaacttctaa aaggcttctc gcatttcact cttggtaacg gtgtgatgat
     2461 ctttgcggca ttggttttaa tttacctggc tgtcttcaaa gaaattgaac ctgttttgtt
     2521 attgccgatc ggttttgggt gtttgctggc aaatattcca ctcgctggta tgaccgctgc
     2581 agaaggcatg accaaggtga tttatgatgc tggcatcaag actgaacttt ttccactgct
     2641 gatttttatt ggcgtcggtg caatgatcga cttctcgcca ctgttggcac aaccacgcat
     2701 ggttttgctc ggtgcagcag gacagttcgg catattcggc accttgatgc tggctgtagc
     2761 attgggcttt ccgctcaatc aagctgcgtc tataggtgtg atcggcgcca ttgacggacc
     2821 gacatctatt tttgtagcaa ccaaacttgc gcctgaattg cttgctccta tcgcggtggc
     2881 agcatattcc tatatgagtc tgattcccat cattcagcca ccgttgatga agctctttac
     2941 cacaaaaaaa gaacgtttga ttcagatgga atacacacct acacccatct cgaaaacaac
     3001 gctgatcttc ttccctgtgg tattgacact ggttgtgggt ctgcttgtgc ctgaagcgac
     3061 gccgcttatt tcgatgctca tgctcgggaa tttgctaaaa gtcacaggtg tcgttgatcg
     3121 cttgagcaat gccgcacaga atgagattat caacatcgcg acattgtttc ttggtttagc
     3181 catcggttca acgatgaatg cggctgcttt tctgaacttg gcgaccggca aaatcatgtt
     3241 actcggtcta ttcgccttcg cgcttgatac ggttgcaggg ttgttatttg gaaaattcat
     3301 ggcattcatc acaggcggga agatcaatcc gcttattggg gcggctggca tctctgcctt
     3361 tccgatggca gggcgccttg ctgcaaaaac cgccaatgat gaagataata acaactttat
     3421 tctcatgcat gccatgggcg cgaatacagc cggtcaacta ggaagcgtga tcgcgggtgg
     3481 aatattgctt tctgtagtta gcaaaatact aggcatttaa ggaactcaac tggctttttt
     3541 gctacaatga aactgaagaa agaggcgcaa atgaagctgg aagggtcatt catctttgca
     3601 agtttagctt ttctgtttgc aattggttcc atcatttatc tgaaacgcat gcaaaatgtg
     3661 atagaaaatg acataaaaca cagaatacga acagagtatc cgccagagag ccgaaaacaa
     3721 attttggaat tctatcggcg tctaaaaatt aaagaaatgg aagggctttt ttccaaaatt
     3781 cttgacgacg ccaaagggga tgtaaacaag gttgggaagc taacaaatgt cgctgaaagc
     3841 atagggtgga aagcatttct tgaaaacgag tggtgataaa aagaaacagg gctaagaaat
     3901 tcgccctgtt tctttttatc cattcccgtt ctggtctttt cccggtttta cttcgctatc
     3961 tagcactcgt tgaaaaaaat ttctggtttc atcaagcaca attgtgttta accgatccaa
     4021 ttcttttacc aagtctctat cttctggctt ggttgcaagg aaacggtctc gttctttctc
     4081 aagacgagtg ccatttaaga ccatatcccg ccaggtagca ccccaatgga agttctgtcc
     4141 caatccattg atcaccgtca acgtggcagc gatgaccgga gttgccacag caaaagcgac
     4201 tctcagccac tccggctttt gcacaaattc tgcggctgag atcgatgaaa ccaaggtcac
     4261 gagtgcaccg agaatcactg tccaagcacg gtagcgttta tacgagtttt tattactacg
     4321 gctggcgccc caataatagg aaatcttgtt gttgtactta tctaaaacat atttatcatc
     4381 aataggttca tttgggttga gttgctcatt ttcagtcatt atcatacctc cacatcggga
     4441 tgcgtttttt cttgccacta ttatgacact tttttacaaa atttcacagg aattttttac
     4501 cctcacaaga gtcaatgtta gaatcttgct tcacacaacc tatcgctaaa tcactaagga
     4561 gtcaggtatg accgcatttg taattctaga cattgaagta acagaccctg ttggatacga
     4621 agattataaa aaactggcag tgccttctct cgaaccctac ggcgggaaat atgcagcccg
     4681 tggtggaaaa gttgaaaacc ttgaaggtga ttggtcaccc acacgtattg ttgtgatcca
     4741 atttgaaagc gtggaacgcg ccaaagcatg gattaattcg ccagaataca gcgaagcgcg
     4801 tgctttacgg cataaatacg cagtctcgaa agccattgtg gtggaagggc tataaattga
     4861 agtc
//
LOCUS       DAOQJE010000156         4840 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_10366_length_4840_cov_6.266876, whole genome shotgun sequence.
ACCESSION   DAOQJE010000156 DAOQJE010000000
VERSION     DAOQJE010000156.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 4840)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4840)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4840
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10366_length_4840_cov_6.266876"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2641)
                     /locus_tag="PLT08_05540"
     CDS             complement(<1..2641)
                     /locus_tag="PLT08_05540"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HNE03958.1"
                     /translation="MKFKPISSPLRTALIYLIFGGLWILSSDTVLGLLVSHSSDLYAQ
                     IQTIKGWLFVLVGAVLLYSILSVDMKALHKREEEIRQSHNLYQQLFDSNPQPMWVYDL
                     DTLRFLMVNHAALEHYGYTETEFLSMTIKDIRPPEQLAALTANLAQPPQPVERSVGWV
                     HRKKDGSVIDVEISSHAIDFKGRPARMVLANDVTERKKIESALQASEAQFRNLVERTP
                     AITYISTLDEISTTKYISPQIEAVLGFSPKEFEAIPHLWEQQLHPEDHQYTIQTFQES
                     QKEGRSFICEYRMISKQGEVVWFRDQANLVRNENGKPLFYEGFMLVITEQKLAEQALQ
                     ASEIRFRALFEDVPIAIWEEDFSAVKEHIDLLKTQGVTDFRAYLTAHPNRVHEFMEKA
                     KVLNVNRTALNLYKAASKEELIASTTAELSPGEAENNIEDLIAIAEGRLGNHWEGADT
                     TLKGEKLQINLQWSVVPGHETDYSKVIVTTVDITERKQAEEALRVSEENYRGLVESSD
                     NAIAVFDRDGNVLYANQSSQQLWNAPELVGKTLYDLFQKDFADHHLISMKKVIDEQII
                     DTNELEVVIHGNKMWFHVTMSPLKNSGGSVDRLLLNALNLTERKKAEEAQRRSEILFF
                     KIFRASSIGIHLFRLSDGRSYNVNDAFLEIIGYPREELIGRTAVELNLFEDIETRNRW
                     MQALREGRSVRNQDARIRRKSGEIRDCLASLDLIDVSGEVMVLVIATDITERKRAEVQ
                     LRQSEERYRTTLDSMLEGIQIIDSDWRYTYINEAAAKQGQSSPEQLLGRTMMEAYPGI
                     EHTELFSILRDCMTHRTVKRFENKFIFPDGFSGWFELNVQPAPEGIFILSVDVTERKK
                     AEEELRGSEERFRLMAENIEEGFW"
     gene            2988..3362
                     /locus_tag="PLT08_05545"
     CDS             2988..3362
                     /locus_tag="PLT08_05545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE03959.1"
                     /translation="MSTCALSVSDDKKFIIMKITGQVNSQDMYQHTLEAHQLGRSLHL
                     RAFLVDLTDAINTQSVVEKYDFVYKKVQEDDELLHFVRMAMWVKKGDHSHDFIETLAN
                     NTGEQAKIFHDQQAAIRFLLEP"
     gene            complement(3442..4026)
                     /locus_tag="PLT08_05550"
     CDS             complement(3442..4026)
                     /locus_tag="PLT08_05550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE03960.1"
                     /translation="MNKNLRILSVLGVQVLIVAALLFGAQKLTEYQNANVPPKTYVYK
                     NIYFDYPGNWELKLEESDQGGELTVTPAKGSPEEKMFGQFYIMFLPDIAFDENWKQTL
                     ADEASCSGKENTQWHQMIEGDDFSGFECVWKDPERAFPSWEFFLYNEGEQTAISILAL
                     PVNDTATEALQSPETVQQVFPDLLALAKSLRISK"
     gene            complement(4023..>4840)
                     /locus_tag="PLT08_05555"
     CDS             complement(4023..>4840)
                     /locus_tag="PLT08_05555"
                     /inference="COORDINATES: protein motif:HMM:TIGR03862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TIGR03862 family flavoprotein"
                     /protein_id="HNE03961.1"
                     /translation="GLPADTSLHFETPNGNVTVKADAVILALGGGSWRKLGSDGAWVE
                     WLSQAGVKVEPLRPANCGFDVAWSSVFKEKFDGQPVKSVIVSFGAFHQQGEFIITQEG
                     VEGSLVYAASALLRDAIEASGTATMTLDLAPDRTEAQLVERLSKPRGSRSMASHLEKA
                     VGIKGVKAGLLREFVPKEEFTNEVRLAFYIKNLPVPLVATRPLDEAISSAGGVRFEML
                     DQGLMLKHHPGIFCAGEMIDWEAPTGGYLLTACFASGRWAGAGVKKWLSGDME"
BASE COUNT         1188 a         1194 c         1138 g         1320 t
ORIGIN      
        1 tccaaaaacc ttcttcaatg ttttcagcca tcaagcggaa gcgttcttct gaccctcgca
       61 gttcttcttc agctttctta cgctctgtga catctaccga aaggataaaa atgccttctg
      121 gggcaggttg cacattgagt tcaaaccaac cactaaaccc atctgggaag atgaatttgt
      181 tttcaaagcg tttgaccgtg cggtgggtca tgcagtcacg cagaatggaa aaaagttccg
      241 tgtgctcaat gccggggtac gcttccatca tggtgcgccc caggagttgt tcaggcgagc
      301 tttgcccttg cttggcggca gcttcattga tgtaggtgta gcgccaatcg gagtcgatga
      361 tctgaatccc ttccaacata ctatctagag tggtgcggta gcgttcttcg ctttgtctta
      421 attggacttc ggcgcgttta cgttcggtaa tgtcggtggc aatcaccagc accatgacct
      481 cgccgctgac atcgatcaag tccagcgatg cgaggcagtc tcgaatctcg ccagatttgc
      541 gcctgattct ggcatcttga ttgcggacgc tcctgccttc tcgtagggct tgcatccacc
      601 tatttcgcgt ctcaatatct tcaaacagat ttaactctac ggcagtccgc ccgatcaatt
      661 cttcgcgagg gtaaccgatg atctcaagaa acgcatcatt gacattatag gagcgcccat
      721 cggaaagacg gaagagatgg atgccgatgg aactggcacg gaagatctta aagaatagaa
      781 tctctgatcg gcgttgcgct tcctcggctt ttttgcgctc ggtgaggttg agcgcgttta
      841 acaacagcct gtccacagag ccgcctgaat tttttagcgg gctcatggtg acatggaacc
      901 acatcttgtt tccatggatc acgacttcca gttcattggt gtcgatgatc tgttcatcga
      961 tgaccttttt catggaaatg agatggtggt ctgcaaagtc tttttggaag aggtcgtaca
     1021 gggtcttgcc gaccagttcg ggggcgttcc ataattgctg gctggattgg tttgcataaa
     1081 gcacattgcc gtctcgatca aacacggcaa tcgcgttatc agaagactct accaacccgc
     1141 gataattttc ttcactcacg cgcaaggctt cttcagcttg tttgcgttca gtgatatcaa
     1201 cggtggtgac gatcactttg gaatagtcgg tttcatgccc cggcactaca gaccattgca
     1261 ggttgatctg gagcttttcg ccttttaggg tggtatcggc tccttcccag tgattgccaa
     1321 gcctgccttc ggcaatggca atgaggtctt caatattatt ttccgcttcg ccggggctga
     1381 gttctgcggt tgtgctggcg attaactcct ctttgctggc tgccttatac aggttaagcg
     1441 cggtgcggtt gacgttcaac acctttgcct tttccataaa ctcatgcacc ctgttagggt
     1501 gggctgttaa ataggctcgg aaatcagtaa ccccttgtgt ttttagaaga tcgatgtgtt
     1561 ctttgaccgc tgaaaaatct tcttcccaaa tggcaatggg cacatcttca aacaaggcac
     1621 ggaagcggat ttcacttgcc tgtagggctt gttctgccag cttttgttcg gtgatgacca
     1681 acataaagcc ctcataaaag agcggctttc cattttcatt cctgaccaag ttggcttggt
     1741 ctcggaacca aacgacctcg ccttgcttgc tgatcatgcg atattcacag ataaaagaac
     1801 gcccctcttt ttgactctct tgaaaggtct gaattgtata ttgatgatct tccgggtgga
     1861 gttgttgctc ccataaatga gggatcgctt caaattcttt gggagagaac cccaatacgg
     1921 cttcgatctg tggactgatg tattttgtgg tgctgatttc gtcgagggtg gaaatgtagg
     1981 tgattgcggg cgtgcgttcg accagattac gaaactgggc ttctgaggct tgcagggcgg
     2041 attcgatctt ttttcgttcg gtgacatcat ttgccaagac catgcgggca ggtctgcctt
     2101 taaagtcgat ggcgtgcgag gagatttcga catcgatcac ggagccgtct ttcttgcggt
     2161 gcacccagcc aacggatctt tccacgggtt ggggcggctg tgccagattt gccgtgaggg
     2221 cagctaactg ctctggcgga cgaatatcct tgatggtcat gctcaggaat tcagtttctg
     2281 tatagccata gtgctctaac gcggcgtgat tgaccattaa gaagcgcaga gtatcaagat
     2341 catagaccca catgggctgt gggttgctat cgaataactg ttgatatagg ttgtggcttt
     2401 gcctgatttc ctcttcgcgc ttgtgtaggg ctttcatatc tacagataag atcgaataca
     2461 agagcacggc accgaccagc acgaaaagcc agcctttaat ggtttgtatc tgtgcgtaga
     2521 gatcagagga atgggatacc agcagaccaa gcacggtatc actggaaaga atccatagtc
     2581 ccccaaagat caggtagatc agtgcggttc gtagtgggct tgagataggc ttgaacttca
     2641 caaactacct ccatgcaaca aaatgttaaa cctctaaaag ttaaccaata aaaaaagtat
     2701 agcacttcag gctttgcctg acattgttac taagtaacaa gattatagtg taagttgacc
     2761 tcctgttgac gatttgacct cctattccac ccttaaacca agagacagcc cctgctacgg
     2821 gactgtctct tttggtttgg agctggtttt accaaaattt gaacaattgg caaagtaggt
     2881 tacaatgtaa agctaggaat gtatacaagt catagagaaa attgattgaa acgtagccaa
     2941 atataaaata caatggaagt aggtcttaat agggatagga gaccactatg agcacttgtg
     3001 cgctttctgt ttcagatgat aaaaaattca tcatcatgaa aattaccggg caggtcaata
     3061 gtcaggacat gtaccagcat acattggagg cgcatcaatt ggggcgttcc cttcatctgc
     3121 gcgcctttct cgtcgacctg accgatgcca tcaataccca atccgtagtc gaaaaatatg
     3181 attttgtcta taaaaaagtt caggaggatg acgagttgct tcatttcgtt cgtatggcga
     3241 tgtgggtgaa aaagggcgac cattcccatg attttatcga aacccttgcc aacaacacag
     3301 gggagcaagc caaaatattt cacgaccaac aagcggctat ccgcttttta cttgaaccgt
     3361 agaacgtgct ccatccatca aaaaggacat ccctcgagcc atatcgaggg atgtcctttt
     3421 gatagacaag acaaaaattg actacttaga aatgcgtaaa ctttttgcaa gcgccagcag
     3481 gtctgggaat acttgctgga cggtttcagg gctttgtaga gcttcggttg ccgtatcatt
     3541 gaccggcaag gctagaatac tgatggcggt ttgttcgcct tcgttgtaga ggaaaaactc
     3601 ccaactgggg aaggctcttt cggggtcttt ccacacgcac tcaaagcccg agaaatcatc
     3661 cccttcgatc atctgatgcc actgagtgtt ttctttccca gaacaactgg cttcgtctgc
     3721 cagagtttgc ttccagtttt catcaaaggc aatatcgggc agaaacataa tatagaactg
     3781 cccaaacatt ttttcttccg gcgagccctt cgcgggtgtg acggtcagtt cgccgccttg
     3841 gtcgctttct tccaacttga gttcccaatt gccggggtaa tcaaaataga tattcttata
     3901 cacataggtc ttgggtggga cgtttgcatt ctgatactca gtcaactttt gtgcgccgaa
     3961 caataatgcc gcaacgatca acacttgcac cccaagcacg ctcaaaattc taagattctt
     4021 attcattcca tgtctccaga taaccatttt tttacgccag cacctgccca acgtccgctt
     4081 gcaaaacaag cagtcagcaa ataaccgccc gtgggagctt cccaatctat catttcaccg
     4141 gcgcaaaaaa tgccggggtg atgttttagc atcaagcctt gatccaacat ctcaaatctc
     4201 acgccgccag cagaactaat cgcctcatcc aacggacgag tcgcaaccag tggcacaggc
     4261 aggttcttga tatagaaagc cagccgtact tcatttgtaa attcttcttt gggtacgaac
     4321 tcacgcaata acccggcttt tacccctttt atgcctacgg ctttttccaa atggcttgcc
     4381 atcgagcgcg acccacgtgg ttttgataac cgctctacca actgagcttc tgttcggtct
     4441 ggtgcaaggt ctagagtcat tgtggctgta ccgcttgctt caatcgcatc acgtaacaat
     4501 gctgaagcgg cgtataccaa acttccctcc acgccttctt gtgtgatgat aaattcgccc
     4561 tgctgatgaa atgccccaaa cgatacgatc acagacttga ctggctgccc gtcaaacttt
     4621 tctttgaata ccgaactcca tgccacatca aacccgcaat tggctggacg taacggctcg
     4681 accttaaccc cggcttgact caaccattcg acccatgccc cgtccgagcc gagtttgcgc
     4741 caactgccgc cgcccaaagc aaggatgacc gcatcagctt tcacagtgac attgccattg
     4801 ggtgtttcaa aatgtagaga cgtatcggct ggcaacccgc
//
LOCUS       DAOQJE010000157         4830 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_10391_length_4830_cov_6.029110, whole genome shotgun sequence.
ACCESSION   DAOQJE010000157 DAOQJE010000000
VERSION     DAOQJE010000157.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 4830)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4830)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4830
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10391_length_4830_cov_6.029110"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1298
                     /locus_tag="PLT08_05560"
     CDS             <1..1298
                     /locus_tag="PLT08_05560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE03962.1"
                     /translation="FEVLDSHFTWSHFPKQVKIDDADIKQWMISLPLTNLPRITQSIS
                     NLPTSPDEKELEEAIRETITTSWKSLSYSQIDLAVNTFMNCIREALLPLKKQTLLIVG
                     RAVLRTEDKINLLIKLTEKLIEQSSKPSTTNTTNPDKQFSKPDTRQETLYSNLLKIES
                     YAKRIYVAPTHARGRNEIFRNLLKLSGHANSEFILKNKTIISFHDLCSYPWTEVCDSS
                     LAIEYSSAEWALSEKIDKRNEFIDLLNKSIEAKLYPEVLFDREEKYYYFAPTEDLRDK
                     ELSYPSLQKNTSRFVFKAYIKKTEQKIGYYRHSAFYGKFLRLDNNWYLEISPHYRFTK
                     NGYWLSRFSDKQISTIKRVELNPAVLGQIVMWSEYLSRRPDMFTKEYPFLSFSDLESF
                     EVDFGFDDDSWLKREDEDNLMLDVVKSSVNDLPLFKGMK"
     gene            1305..2750
                     /locus_tag="PLT08_05565"
     CDS             1305..2750
                     /locus_tag="PLT08_05565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE03963.1"
                     /translation="MKIEFIDEPELEFGTGRSVDIRFGLTNYHPVDFDNRIAPKSIRV
                     GIVGNNETIEGMAGWLEKCRNEIQAKQSNQPNLFPKFPGFRLDTGFNSELILDSSLQR
                     IIPQRQIDEALKAGNIKDKVINAVNLYLDELSYLNENAKVDVLICAIPEEVFNIGQPV
                     SDESDEDEAASYSRSISSIDFHDLLKAKAMRLGVPIQLVLPSTYDSKKRRTSRNNPAL
                     TRMLQDEATRAWNFHTAIYYKAKGIPWRLLRNPSDYSTCYVGVTFFKTDNDMLRTSVA
                     QVFNERGEGVIVRGGLARFDKEDRQVHLEQKDATTLLTNSLNLYKKEHGAFPARLVLH
                     KSSTYNKSEVDGFLESASSFSVDNVNLLSVEKSFTRLFRMGKYPPLRGTFLSLDKKQH
                     ILYTRGSVPFFATYPGMYVPKTFGFKTEIIEETPYFLAKEILALTKMNWNNTQFDGGE
                     PITTRASRQVGKILKYIEDGDPIAPSYRYYM"
     gene            3418..3999
                     /locus_tag="PLT08_05570"
     CDS             3418..3999
                     /locus_tag="PLT08_05570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE03964.1"
                     /translation="MKYLSVALIALIMAGCGPSSPEMTATADMAMAETQTAAPTSTPT
                     LTPTSTATPTSTTTPILTLTPTLPHEAPGIDPGGMPESANSLFFESTPMFTFNLGGSV
                     KVNYIVFLEIKKELPKDAILEVHFQNPADPSTAYVVVVTDLTGEKIMVESKKFPMSNF
                     ECRNYWIDVHIYADRESIYELGTHVQWTSSSFC"
     gene            complement(4094..4303)
                     /locus_tag="PLT08_05575"
     CDS             complement(4094..4303)
                     /locus_tag="PLT08_05575"
                     /inference="COORDINATES: protein motif:HMM:NF014043.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF86 domain-containing protein"
                     /protein_id="HNE03965.1"
                     /translation="MIGEAASRLPKEFTARFPEIPWVDIIGFRNIAVHEYFSIRWDIA
                     WIAATEEVPVLREQVEKILREEFAE"
     gene            complement(4293..4439)
                     /locus_tag="PLT08_05580"
     CDS             complement(4293..4439)
                     /locus_tag="PLT08_05580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE03966.1"
                     /translation="MRPEKLYLTDIVEAAQAIERFVMGESYYEFEQNEMMNSAVLQKI
                     DRDR"
     gene            complement(4429..4746)
                     /locus_tag="PLT08_05585"
     CDS             complement(4429..4746)
                     /locus_tag="PLT08_05585"
                     /inference="COORDINATES: protein motif:HMM:NF014021.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HNE03967.1"
                     /translation="MDEKTSSRVKIRVPKKELADFCRRYNVQKLALFGSVLGNNFRAD
                     SDVDVLVSFQPNAKIGFITFSRMQRELAELFNRPVDLVPMDGLKPVIRDSVLSDIEEV
                     YAS"
BASE COUNT         1529 a         1081 c          946 g         1274 t
ORIGIN      
        1 tttttgaggt gctcgactct cattttactt ggtcacattt tccaaagcag gtcaaaatcg
       61 acgacgccga tattaagcag tggatgatat cgctgcccct tacaaatcta cctagaataa
      121 ctcagtcaat ctcgaatcta ccaacctctc ccgacgagaa agagctggaa gaagctataa
      181 gagagacgat tacgacctct tggaaaagtc tctcttattc gcaaatcgat ctagcagtta
      241 atacattcat gaattgtatt cgtgaagcgt tactgccgct gaaaaaacaa acattgctaa
      301 tagtagggcg tgctgttcta agaactgaag ataaaatcaa tttacttatc aagcttacag
      361 agaaattaat agaacagtct tcaaaaccat ccaccacaaa cacaaccaat cctgataaac
      421 aattctcaaa gccagacaca cgtcaggaaa cgctatattc aaacttattg aaaattgaga
      481 gctacgccaa aagaatatac gttgccccga cacacgcacg cgggcggaat gaaatattta
      541 gaaacctact taagttaagt gggcacgcca acagcgaatt tatacttaag aataaaacta
      601 ttatttcttt tcacgacttg tgttcatatc catggactga agtatgcgac tcatcattgg
      661 ctattgagta tagtagcgca gagtgggcac tttcagaaaa aatagataaa agaaatgaat
      721 ttatcgactt attaaacaaa tctattgaag caaaattgta cccagaagta ttgtttgatc
      781 gagaagaaaa atactattat tttgcaccga cagaagattt gcgagacaaa gagctatcgt
      841 acccaagctt acagaaaaac acttctcgtt ttgtgttcaa agcttacata aagaaaactg
      901 agcaaaaaat cggatattat cgtcactcag cattttacgg aaaattccta cgactagaca
      961 ataattggta tcttgaaatc tcgccacatt atcgtttcac taaaaatgga tactggctat
     1021 cgagattttc agacaaacag atttcaacca tcaaacgagt tgaattaaat cctgcagttt
     1081 taggtcaaat agttatgtgg tctgaatact taagccgacg cccagatatg tttacgaaag
     1141 agtatccatt tttatctttt agcgatctag agtccttcga agttgatttt ggtttcgacg
     1201 atgattcgtg gctaaaacgc gaagatgaag acaatctaat gttagatgtg gtcaaatcct
     1261 ctgtgaacga cttgcctctt tttaaaggga tgaaataatt tctcatgaaa atagaattta
     1321 tcgatgagcc agaacttgaa ttcggaacag gtcgcagtgt ggatattcga tttggtttaa
     1381 ctaattatca ccctgtagat tttgacaatc gaattgctcc caaatcaata agggttggca
     1441 ttgttggaaa taacgaaaca atagaaggaa tggctggctg gcttgaaaaa tgtcgtaacg
     1501 aaattcaagc taaacaaagc aatcaaccta atttattccc aaaatttcca gggtttaggc
     1561 ttgatactgg ctttaattcg gagttgatac ttgactcgag tctacaaaga attattcctc
     1621 aaagacaaat agacgaagca ttaaaagcag gcaatatcaa agataaagta attaatgcgg
     1681 taaaccttta cctcgatgaa ctttcttatc taaacgaaaa tgcaaaggtt gatgttctga
     1741 tttgcgcaat tcccgaagaa gtcttcaata ttggacaacc agtatcagat gaatccgacg
     1801 aggatgaagc ggcaagttac agcagaagca tttcttcaat tgactttcac gatctgctaa
     1861 aagcaaaagc catgagattg ggagttccaa ttcaattggt tttaccatct acctatgact
     1921 cgaaaaagag gcggacatcg cgtaacaacc ccgctcttac gcgcatgttg caagatgaag
     1981 cgactcgagc ttggaatttt cacacagcca tatactataa agcaaagggt attccatggc
     2041 gtttattgag gaatccatca gattattcca cgtgttatgt tggagtaacc tttttcaaga
     2101 cagataatga tatgctccga actagtgttg cacaagtttt taacgaaaga ggagaaggtg
     2161 taattgttcg aggtggttta gctcgttttg ataaagaaga taggcaagtc catttagagc
     2221 aaaaagacgc aaccacatta cttacaaact cattaaattt gtataaaaaa gagcatgggg
     2281 cattccccgc acgcctcgtt ttacataaaa gttcaactta caataaatct gaggtcgatg
     2341 gctttttaga aagtgcctct agtttcagcg ttgataatgt taacttattg agtgtggaaa
     2401 agtcattcac aagactattc cgaatgggga aatacccacc attacgggga acatttttat
     2461 ctcttgataa aaaacagcat attttatata caagaggtag tgttccattt ttcgcaacct
     2521 atcctggaat gtacgttcct aaaacttttg gttttaagac cgaaataata gaagagacac
     2581 cttattttct agcaaaagaa atcctagcac ttactaagat gaattggaat aatactcaat
     2641 ttgatggcgg agaaccaata accactcgtg catctcgaca agttggtaaa attttgaagt
     2701 atatagaaga tggagatcct atagccccat catatcgata ctatatgtaa aaacaataat
     2761 acctccgagc ggacaactcg gaggtattac tttccactca ctactttcta cgatcactcc
     2821 cccgacggca cccagatatt cttgacctgc gtcgccttgt gcaggaactc gtgaccttcg
     2881 ccttgctcgc ggtcgagcca attgcgggac gagccgtaac cgacccaaat ccgctgccta
     2941 gcaagcataa catccgattg cctatacgca aatcgaaaca gggggaacaa gaagtcctct
     3001 caggggagga ttggaaacga gtcagggaag gaattaaacc cttccttgag aggatcagaa
     3061 acctcccttg actcgtttta aaaaacatct ataagttttt taaaaacttc tagatgtttt
     3121 cccaaagact tctaaatgtt ttttaaaaac ttcaagaagt ttttccactc aaaacagccc
     3181 atttttcaca aaaaaaggcc tcgaaagcgc ctcgaaattc tgtcccatcc tgtccctggt
     3241 caaacccggt ctggaggcgt ccccacacgg caagatggat gagaacaggg gaaaattcta
     3301 ttacgcaggc caaaattaaa aattggcggt tattggcagt taaaagccgg ggaaaggttg
     3361 tataacgcca taatcaaaac aatcgcatca aacggaatag ccaacaggag aattaccatg
     3421 aaatatttat ccgtagcatt aatcgctttg atcatggctg ggtgcggccc gtcatcgcct
     3481 gagatgacag ccactgccga tatggcaatg gcggaaactc aaaccgcagc ccccacatcc
     3541 acgcctactt tgactccgac atcaaccgca accccaacgt caaccacgac cccgatattg
     3601 accttgactc ccacacttcc acatgaagcg cccggcattg acccgggtgg catgccagaa
     3661 tctgcaaact ctttgttctt tgaaagcacg cccatgttta cattcaacct tggcggttct
     3721 gtaaaggtta attatattgt ttttcttgag ataaaaaagg aattacccaa ggatgcaata
     3781 ctcgaggtcc atttccaaaa cccagctgac ccgtcaacgg cctatgttgt ggtcgtaaca
     3841 gacctaacgg gtgaaaagat aatggtcgaa tccaagaaat tccccatgtc caatttcgaa
     3901 tgcaggaact actggatcga tgtccacatc tatgcagaca gggaatcgat ctacgaatta
     3961 ggcacacatg tccaatggac aagttccagt ttttgctaaa aaacagacct ctgaggccag
     4021 cctcagaggt catctactct tcaatctcta cttccctgct cttccctact cccccgttgg
     4081 cacccagata ttcttattct gcaaactctt cccgcaaaat tttctcgact tgttccctca
     4141 acacagggac ttcctctgtc gccgcaatcc atgcaatatc ccaacgaatg gagaaatact
     4201 catggacggc gatattgcga aaaccgataa tatccaccca cgggatttca ggaaatcgag
     4261 cagtaaattc tttcggcaaa cgggatgcgg cttcaccgat cacggtcaat tttttggagc
     4321 acagcgctat tcatcatctc attttgctcg aactcataat aactctcgcc catgacaaag
     4381 cgctcaatgg cttgggcggc ttcaacgata tccgtcaggt aaagtttctc aggacgcata
     4441 cacttcctcg atatctgaga ggacagagtc gcgtatgacg ggcttcaaac cgtccatggg
     4501 aaccaagtcg acggggcgat tgaacaactc ggcaagttcg cgctgcattc gagagaaagt
     4561 gatgaacccg atttttgcat tgggttgaaa ggacaccagc acatccacat cactatccgc
     4621 gcggaagtta ttcccaagca cagaaccgaa taaagccaat ttctgcacgt tgtaacggcg
     4681 gcagaaatca gccaattctt tcttgggaac tctgatcttc acgcgggaag aagttttctc
     4741 gtccatgcct taattatacc tgtcactccc ccgtcggcac ccagatattc ttgacctgag
     4801 ttgcctcgtg caggaactcg tgaccttcgc
//
LOCUS       DAOQJE010000158         4830 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_10392_length_4830_cov_5.835393, whole genome shotgun sequence.
ACCESSION   DAOQJE010000158 DAOQJE010000000
VERSION     DAOQJE010000158.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 4830)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4830)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4830
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10392_length_4830_cov_5.835393"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1943
                     /locus_tag="PLT08_05590"
     CDS             <1..1943
                     /locus_tag="PLT08_05590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE03968.1"
                     /translation="LHDFYWKGCMINGWPETLLAGLQTINQILTAGIAITAFSLFLYS
                     LSFNLRDRVARSFAIILLCVVIVFTAESLQDNSVTIKALDLLMRLQWFGLAFLPAAYL
                     HLSDALLVTAGRPSRGRRRFAVRGMYIISALFLVLLAFGYLLGPIVPDGKPAPYLQRT
                     AWTEVFTIFYVTSMVWAGVNFYRAYTLMLTRSGRRRMIYLMAGATAPALGSYPYLLFG
                     YSLASQHQFLFWTAATVINALVGALVIVMAYAVAFFGVSWPDRVIKSRLFKWIMRGPV
                     VASATLALMTVVRRGGELLGTPYSAFVPFTVVASILLMEHAITFAAPLWERGLFFGRD
                     RNELELLQNIEERLLTQGDLQQFLEAVLAAVRDHLQSPSAFVAALDDETLLPVVVAGN
                     RALLEQESLTEALEHINGDVRHEFQWGNYWVLPLYSRRRPEMDEDELPPLLGLLGVAK
                     NGKRDIMEQDQRESLWLLADRAALALEDRQLQQRVFRSLADIQPQVEMIQRMRAASRY
                     DTNANLLTAPLPREADLANWVKDALTHYWGGPKFTNNPLTKLQVVQELADKEDGNISN
                     ALRTLLRQAVDEVKPRGDRKFTTEWILYNILEMKFIEGRKVRDVAARLAMSEADLYRK
                     QRVAIEAVAKAILEMEVSKLEQ"
     gene            2037..3002
                     /locus_tag="PLT08_05595"
     CDS             2037..3002
                     /locus_tag="PLT08_05595"
                     /inference="COORDINATES: protein motif:HMM:NF012784.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HNE03969.1"
                     /translation="MNEKTKYDKAEPTIDEGWWESVLAEENRYSTPTQRLVQSKPEVQ
                     KEAGKESVSEEAQITVDWSQIKDLYLKDRIVELTVTGHNRGGLLVEGGGMFGFVPFSH
                     LIELAGKDNLDRSHDLEPYIGRTLHLKVIECVPEDGRVVFSERAAQTEPGKRAELFHS
                     LHAGQSVSGTVTNITDFGVFVDLGGVEGLIHISELSWGRVTHPSQFVKLGNAIDVQVL
                     DLAPERCRVALSLKRMLPNPWTNAANEFPIGSVHNATITSVLSYGVFARLDKYGVEGL
                     VHASEIPHEEGTLLKDFLSDGQAIQVRVLHLDPAHHRLGFSMRLE"
     gene            3006..>4830
                     /locus_tag="PLT08_05600"
     CDS             3006..>4830
                     /locus_tag="PLT08_05600"
                     /inference="COORDINATES: protein motif:HMM:NF013727.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015616 - DNA translocase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HNE03970.1"
                     /translation="MSKPTETRRYAQEDFSPRTDWLGRLARLNTQFGRFTRDLIGVSL
                     ISFSVMAFFAIWNWTDGFVLTPIALILRTWFGWGSLLILAGIAYLGYSFLQRQQTSLH
                     WGRILALELGALLTLGLLAIPFGNDLFRAESGMDGGRLGWGIVRLLWQMGTIPGTLVL
                     LLLWALSVATGFGLWAKLEAWLIKIAGDEAPVIVSMPEPEVPQEIAVEQPVSKEAAAP
                     KKKAPAIPPEFRTQLKSAEKKPEKSLKPPPRGEDLPPLTVLMGDSTVRTDERTINQTA
                     GLIMKTLSEFGIPATVIGYRVGPSVTQFAVQPGFIKKGNEEEDAQQMKVRVAQIASLE
                     KDLALALSAQRLRIEAPVPGKPYVGIEVPNVNSSIVRMRALLESDAFYKVGAPLTIAL
                     GRDVSGNPLMADLSRMPHLLIAGSTGSGKSVCVTAIAACLAMNNTPEELRIVMIDAKM
                     VELIRFNGLPHLYGKVETNLERILGVLRWVVVEMEHRYRLLESMHARDLNSYNKKVSR
                     SADGEQLPRIVVIIDELADLMMSAPDQTEHNLVRLAQMARATGIHLVVATQRPSTDVV
                     TGLIKANFPARLAFAVASGTDSRVILDYTGAESLLGRGDMLF"
BASE COUNT         1085 a         1115 c         1290 g         1340 t
ORIGIN      
        1 cgttgcacga tttttattgg aagggttgca tgattaacgg ttggccagaa acactactgg
       61 cggggcttca aacgatcaat cagattctga cggcgggaat tgccattacg gcattctcgt
      121 tgtttctcta ttcgctttca tttaacctgc gagacagggt ggcacgttca tttgcgatca
      181 ttttgctttg tgttgtgatc gtttttaccg cagagtctct acaagataat tcagtaacta
      241 ttaaggcgct tgacctgtta atgcgcttgc aatggtttgg gctggcgttt ttgccggcgg
      301 cttacttgca tctttcagat gctttgcttg ttacagctgg gcgcccttct cgtggaaggc
      361 gtcgttttgc tgtgcgtggc atgtacatca tttctgcgct tttcctcgtt ttactcgcat
      421 tcggatattt actcggaccc attgtgccag atgggaaacc tgctccttac cttcaacgca
      481 cagcgtggac agaggttttc acgatctttt atgtgacttc gatggtctgg gcgggtgtga
      541 atttttaccg tgcttataca ttgatgctaa cccgttcggg cagacgccgc atgatctacc
      601 tgatggcggg tgcaaccgcc ccggctttgg gctcataccc gtatcttttg tttggatatt
      661 ctctcgcatc acaacatcag ttcctctttt ggaccgctgc aacagtaatc aacgctttgg
      721 ttggtgcttt ggtgatcgtg atggcgtatg cagtggcgtt ctttggcgtt tcatggccag
      781 accgtgtgat taagagccgc ctctttaagt ggatcatgcg tggacctgtc gttgcctctg
      841 ctacactggc attgatgact gtcgtacggc gtggtgggga actgctcgga actccttata
      901 gtgcctttgt cccttttacg gttgtagcat ctattctctt aatggaacat gccatcactt
      961 ttgctgctcc gctttgggaa cggggcttgt tctttgggcg tgaccgcaat gaattggaat
     1021 tattgcaaaa cattgaagag cgcttgctaa cccagggtga ccttcagcaa tttttagaag
     1081 ctgttttggc tgctgtgcgc gaccatttac agtcgccttc tgcttttgtt gcagccctag
     1141 atgatgagac tttattaccg gttgttgtgg cgggaaaccg tgcattgctg gaacaagaaa
     1201 gcttgaccga agctcttgaa cacatcaatg gagatgtacg gcacgaattt cagtggggta
     1261 attattgggt tctgccgctt tattctcgtc gccgacccga aatggatgag gatgaactcc
     1321 cgcctttgtt gggtctatta ggtgtggcaa agaacggcaa acgagacatc atggagcaag
     1381 accagcgcga gtctttgtgg cttttagccg accgtgccgc gttggcattg gaagaccgcc
     1441 aacttcaaca gcgggttttc cgttcattgg cagatattca acctcaagtg gaaatgatcc
     1501 aacggatgcg tgcagccagc cgttatgata ccaacgccaa tttattgacg gcgcctttgc
     1561 cccgtgaagc tgaccttgcg aattgggtca aagatgcgct tacgcattat tggggtggac
     1621 ccaagttcac gaataatcca cttacgaagt tgcaggtggt gcaagaactg gcagacaagg
     1681 aagatgggaa tatctcaaat gccttgcgaa ccttgttacg tcaagcagtg gatgaagtca
     1741 aaccgcgtgg agatcgtaaa ttcaccaccg agtggatctt gtacaatatc cttgaaatga
     1801 aatttattga agggcgtaaa gtccgtgatg tggcggcgcg gcttgccatg tctgaagcgg
     1861 atttatatcg caaacagcgt gtggcaattg aagcggttgc caaggcaatt ctcgagatgg
     1921 aagtgagcaa gctcgagcag taagtttttt attggaagat ctcgtcgaag aatcctgatg
     1981 tatctctagg agggcgcatt gggatttagg gtgcggcgat ttataaggag aatgttatga
     2041 atgaaaaaac caagtacgac aaagcagaac caacgatcga tgaaggctgg tgggagtcgg
     2101 tccttgccga ggagaacagg tactcgacgc cgacacagcg tctggtgcag tccaagcccg
     2161 aggtccagaa agaggcgggc aaggagtctg tttctgaaga agctcaaatc acagttgatt
     2221 ggagtcagat caaggacttg tacttaaagg atcgtatcgt tgaactgact gtcaccgggc
     2281 ataatcgagg tgggctattg gtggaaggtg gcgggatgtt tggctttgtc ccattttcac
     2341 atttgatcga actggcaggc aaagataatc ttgaccgcag tcacgatctt gaaccttata
     2401 ttgggcgtac tctccacttg aaagtgattg aatgtgttcc agaagatgga cgtgtggttt
     2461 tttcggaacg tgctgctcag acggaacccg gaaaacgcgc tgagttattc cactcattgc
     2521 atgcaggtca aagcgtaagt ggaacagtta cgaacattac agactttggt gtgttcgtag
     2581 accttggcgg tgtggaaggt ttgattcata tctctgaact ttcttggggg cgtgtaacgc
     2641 acccctcgca atttgtaaaa cttggcaatg ccattgatgt gcaagtatta gaccttgcgc
     2701 cagaacgctg ccgtgttgcc ttaagtttga agcgcatgct tcccaaccct tggacgaatg
     2761 cggcaaatga attcccgatt gggtctgtgc ataacgcaac catcaccagc gttttatctt
     2821 atggcgtgtt tgcacggctg gataaatatg gtgtggaagg cttggttcat gcttctgaaa
     2881 ttccgcatga agaaggcaca ttgctaaaag actttctctc tgacgggcaa gcgattcagg
     2941 tgcgtgtatt gcatctcgac cctgcacatc accgccttgg cttcagcatg aggcttgaat
     3001 aagggatgtc taaacccact gaaacaaggc gttacgctca agaagatttt tctccgcgca
     3061 ccgattggct cggaaggctt gcgcgtctta acacgcaatt tgggcgtttc actcgcgatc
     3121 ttattggtgt aagcctaatc tcgttttcag tcatggcatt ctttgccatt tggaactgga
     3181 ccgatggctt tgtgcttaca ccaatcgcct tgatattgcg tacttggttt gggtggggta
     3241 gtctcttgat tttggcaggg attgcttatc ttggatattc ctttttacaa cgccaacaga
     3301 cgtctctaca ctgggggcgg attcttgctc ttgaactcgg cgctctctta accctgggct
     3361 tgcttgctat accctttggc aatgacctct tccgagccga atctggcatg gacggtggaa
     3421 ggttggggtg gggtattgtg cgcctgctgt ggcaaatggg aaccatcccc ggcacgttgg
     3481 tgcttttgct tttgtgggcg ttgagtgttg ctactgggtt tgggctgtgg gcaaagcttg
     3541 aagcatggct cataaaaatt gcaggtgacg aagcgcctgt gatcgtctct atgccagagc
     3601 cagaagttcc acaagagatc gctgtcgaac agccagtttc aaaagaagct gctgctccga
     3661 aaaagaaagc tcctgccatt ccgcctgagt ttcgtactca gttgaaatct gctgaaaaga
     3721 agcccgagaa atcactcaag ccgcctccac gcggtgaaga tttaccgcct ctcactgttt
     3781 tgatgggcga ctcaactgtc cgcaccgatg aacgcacgat caaccaaact gccggtttga
     3841 tcatgaagac tctttcagag ttcggtattc ccgcaacggt aatcgggtat cgagttggtc
     3901 catctgtgac gcagttcgct gttcagccgg gcttcatcaa aaaaggtaat gaagaagaag
     3961 atgcccaaca gatgaaggtt cgtgtggcgc aaatcgcttc actggagaaa gaccttgcgt
     4021 tggcgctatc cgctcaacgt ctacgtattg aggcgccagt tccgggtaaa ccttatgttg
     4081 gtatcgaagt cccgaacgtc aacagttcta tcgtccgcat gcgagccttg ttggaaagcg
     4141 atgctttcta taaagttggc gctccgttga ccattgccct gggacgtgat gtctctggca
     4201 acccgttgat ggcagattta tctcgtatgc cgcacttatt gatcgcaggt tctacgggat
     4261 ctggtaaatc ggtgtgtgtt actgcaattg cggcttgtct tgccatgaac aacacgcccg
     4321 aagaactgcg cattgtgatg atcgatgcca agatggtgga attgatccgt ttcaatggct
     4381 tgccacatct gtacggaaaa gtggagacca accttgaacg cattcttggt gtgttgcgtt
     4441 gggtggtcgt tgaaatggag catcgctatc gcttgctcga atcgatgcac gcccgtgacc
     4501 tgaattcgta caacaagaag gtctcacgtt ctgccgatgg ggaacaactc ccacgcatcg
     4561 tagtcatcat cgatgaactc gcagacttga tgatgtctgc gccagaccag accgaacata
     4621 acctcgtccg cttggcacaa atggcacgtg ctacaggaat tcaccttgtg gttgcgaccc
     4681 aacgtccatc caccgatgta gtgacgggtc ttatcaaagc gaacttccca gcgcgtcttg
     4741 cctttgcagt tgcatcgggc accgatagcc gcgttattct cgattacacc ggcgccgagt
     4801 ctttgcttgg tcgcggtgat atgctcttcc
//
LOCUS       DAOQJE010000159         4791 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_10512_length_4791_cov_2.975929, whole genome shotgun sequence.
ACCESSION   DAOQJE010000159 DAOQJE010000000
VERSION     DAOQJE010000159.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 4791)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4791)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4791
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10512_length_4791_cov_2.975929"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..461
                     /locus_tag="PLT08_05605"
     CDS             <1..461
                     /locus_tag="PLT08_05605"
                     /inference="COORDINATES: protein motif:HMM:NF012695.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HNE03971.1"
                     /translation="TNTGGRVKRLAEFIGKQTFMLTYGDGVGNIDIPKLLEFHHKQGR
                     LATLTAVRPPARFGQMVLSGEKVVEFKEKPQIGEGWINGGFFVLQPEIINYIEGDSTF
                     WEFDSLEKIAAAGGLSAYQHQGFWQSMDTQRDVHLLNRLWQEGNAPWKIW"
     gene            446..1438
                     /locus_tag="PLT08_05610"
     CDS             446..1438
                     /locus_tag="PLT08_05610"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: protein motif:HMM:NF027681.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HNE03972.1"
                     /translation="MEDLVNRAFWLDRPTFVTGSTGLVGSWLVKRLVESGADVVCLVR
                     DWVPQSELVRNGLIEKVKVVRGDIRDRDILERTLGEYEIDTVVHLAAQTIVTIANRNP
                     VSTFETNIAGTWNVLEAARRSPKVRQIVMASSDKAYGDQEILPYDEKTPLQGQHPYDV
                     SKSAADLIASAYAKSYDLPVVITRCGNFYGGGDLNWNRIIPGTIRSIMRGQRPVIRSD
                     GQFVRDYFYVEDGAAAYMLLAEQLASNRSLKGESFNFSNEIQVTVLEIVEKILNLMGS
                     DLKPEILNEASNEIRHQYLSADKAHRILNWKPLFNLDEALKLTIDWYKEFLSNE"
     gene            1431..2747
                     /gene="rfbH"
                     /locus_tag="PLT08_05615"
     CDS             1431..2747
                     /gene="rfbH"
                     /locus_tag="PLT08_05615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017944024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide biosynthesis protein RfbH"
                     /protein_id="HNE03973.1"
                     /translation="MSDRSNELRAKILELTAEYVAEEFPARKFIPGESSVPVAGRVFD
                     TADVQSLIDSSLDFWLTEGRFARQFEKEFARFFNMRHSILVNSGSSANLLALTSLTSP
                     LLKERQLQPGDEVITVATGFPTTVNPIFQNGLVPVFLDVDIPTYNIDVSQLEAALSEK
                     TRAVMIAHTLGNPFDLRTVTEFCKKHNLWLVEDCCDAVGALYDNRMLATFGDVATVSF
                     YPAHHITMGEGGALLTDKPKLKKIIESFRDWGRDCWCEPGKDNTCYKRFDWQLGDLPQ
                     GYDHKYTYSHIGYNLKATDMQAAVGVSQLKKLNVFIEKRRANFDYLTELLSPLEDFFI
                     FPKATPNSTPSWFGFPIFVRPDAPFARDELIRHLEAHKIGTRLLFGGNLIRQPAYREK
                     EYRVVGDLKNSDEVMHRVFWIGVYPGLSNTMLEYVAETFHHFCDSH"
     gene            2847..3827
                     /locus_tag="PLT08_05620"
     CDS             2847..3827
                     /locus_tag="PLT08_05620"
                     /inference="COORDINATES: protein motif:HMM:NF027681.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HNE03974.1"
                     /translation="MSTLIESQKNILQEMELLSDIRGSRVLVTGAGGFVGLHLCEALL
                     DLGAEVHALSRNIESHGLPYRIKTHPVDLQDSHSIKKCVADVKPEVVYHLAGLVNTRQ
                     DLNLVLPTLRNNLVGSVNLFMALAEESCRRLVVVGSSEEPTAGRLGNVANSPYAVSKE
                     AATEYARMFHEIFSLPVVITRPYMSYGPRQPTAKIIPYVITNLLAGTPPEVSSGKRIC
                     DLIYVQDLVMGLVLSGFKPQLTGVSVDLGTGVASTIHDAVGLISDMMNVSVKPKFGAI
                     PDRLYETPQIANSEATFQLLGYRPQWSFRDGIELTVEWYRNHPEFYKTER"
     gene            3834..>4791
                     /locus_tag="PLT08_05625"
     CDS             3834..>4791
                     /locus_tag="PLT08_05625"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: protein motif:HMM:NF027681.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HNE03975.1"
                     /translation="MTWLITGGCGFIGSNLANTLMEQGEQVILLDNLSRVGSQDNLEW
                     LRSRHGSDWAFVQSDIRDSDAVERTIKQHKPKVIAHLAGQVAMTTSLQNPRMDFEING
                     MGTLNVLESVRLHSLDTIVLYSSTNKVYGSLEELDYRETDLRYELTDHPDGLDESLQL
                     DGHSPYGCSKLTGDQYVRDYSRMFGLRTVVFRHSSMYGGRQFATYDQGWIGWFCQKAL
                     EMANPNSPIFTISGDGKQVRDVLHARDLVRVYLQAAKHIDRSSGQIYNIGGGMKNSLS
                     LLELFKLLEENTGFPMRFEKLEWRHGDQKVFVANTEKAKKITW"
BASE COUNT         1283 a          982 c         1171 g         1355 t
ORIGIN      
        1 aaaccaatac tggtggtagg gtaaagaggt tggcggaatt tattggaaaa caaacattta
       61 tgttgaccta tggcgatggt gttggaaata tagatattcc taaacttttg gagtttcatc
      121 ataaacaagg tagacttgcc acacttacag cagtgcgtcc gcctgcaagg tttggacaga
      181 tggtcttaag tggtgagaaa gtggtcgaat ttaaagaaaa acctcaaata ggtgaaggtt
      241 ggatcaatgg cggctttttt gtattacaac ctgagattat taactatatt gagggtgata
      301 gcacgttttg ggagtttgac tctttagaga aaatcgcagc tgctggaggt ttatccgctt
      361 atcagcatca gggattttgg cagtccatgg atactcagcg tgatgtccac ttactcaaca
      421 gactttggca ggaaggaaat gcgccttgga agatttggta aacagagctt tttggctgga
      481 tcgcccaacc tttgtgactg gcagtactgg actggtcgga tcatggctcg taaaacgttt
      541 ggttgaaagc ggagcagatg tcgtctgctt ggtaagagat tgggttccgc agagcgaact
      601 ggttcgaaat ggtttaatcg aaaaagttaa agtagttcgc ggtgatatac gagaccgcga
      661 tattcttgag cgtacacttg gtgagtatga gattgatacc gtcgttcacc tggcagcgca
      721 aacgattgta accattgcca atcgtaaccc ggtttctaca tttgaaacca acattgctgg
      781 aacttggaat gtgcttgagg ctgctcgccg cagtcctaag gtacgtcaga tcgtgatggc
      841 atcatctgat aaggcttatg gcgatcagga aattttgcct tacgatgaaa agacgccgct
      901 tcaggggcag catccatatg atgtgagcaa atctgcagct gatctgatcg cctctgctta
      961 cgctaaaagt tatgatttac cagttgtcat tacccgttgt ggcaattttt atggcggcgg
     1021 cgacttgaac tggaatcgaa ttattcccgg aaccattcgt tccattatgc gcggacagcg
     1081 acctgtgatc cgttccgatg ggcaatttgt tcgtgattat ttttatgtgg aggatggcgc
     1141 ggcagcgtac atgcttttgg cagagcagct tgcttcaaac cgctctctta aaggtgagtc
     1201 ttttaatttt tcaaatgaaa tacaggttac tgtcctggag atcgttgaga aaatccttaa
     1261 tttaatgggg tccgacctta agcccgagat cctcaacgag gcatcaaatg agattcgcca
     1321 tcagtatctc agcgcagaca aggctcaccg catattgaat tggaaaccgt tgtttaacct
     1381 ggatgaagcc ttgaaattaa cgatcgactg gtacaaggaa tttttaagca atgagtgatc
     1441 gatctaatga actccgtgcg aagatactgg agttaaccgc tgaatatgta gcagaagaat
     1501 tccccgcaag aaaattcata cctggcgaat catctgtgcc cgtcgctgga cgcgtattcg
     1561 ataccgcaga tgtacaatcg ttgatcgatt ccagccttga tttttggcta accgaagggc
     1621 gctttgcacg tcagtttgaa aaggaatttg cgcgtttttt caatatgcgg cattccattt
     1681 tagtgaactc tgggtcttct gcaaatttgt tggcgctcac ttcgctgact tcgccgctat
     1741 tgaaagaaag acagcttcaa cctggtgacg aagttatcac agttgcaacg gggtttccaa
     1801 caactgtaaa ccccattttt cagaacggtc tcgttcccgt atttctggat gtggatatcc
     1861 ctacttataa tattgatgtg tctcaacttg aagcagcgct ttctgaaaaa acccgcgctg
     1921 tgatgatcgc tcatacactc ggaaacccgt ttgacctgag gaccgtcact gagttttgta
     1981 agaaacataa cctttggctg gtggaggatt gttgcgacgc agttggcgca ctttacgata
     2041 accgcatgct tgcaaccttt ggtgatgtag caacagtgag tttctatcct gcacatcata
     2101 tcactatggg cgaaggtggt gcgcttctca ccgacaaacc caagctcaaa aagatcatcg
     2161 aatcattccg cgattggggg cgtgattgct ggtgcgagcc gggaaaagat aacacttgtt
     2221 ataagcgttt cgattggcag cttggcgacc ttccccaagg atatgaccat aagtacactt
     2281 actcgcatat tgggtacaac ttaaaagcga ccgatatgca ggctgctgtt ggcgtttcgc
     2341 aactcaaaaa attaaatgtc tttattgaaa aacgccgtgc taatttcgac tacctaaccg
     2401 aacttttatc gcctttggag gatttcttca tctttccgaa ggcgactcca aactcaactc
     2461 caagttggtt tggcttccca atttttgtcc gccctgatgc tccttttgca cgcgatgaat
     2521 tgatccgtca ccttgaggcg cacaagatcg gtacgcgcct tctgttcggt ggaaatttga
     2581 tacgccagcc tgcttatcgc gagaaagagt atcgtgtagt tggcgacctg aagaattcag
     2641 atgaagtgat gcatcgtgtg ttttggattg gggtataccc tggtctttcc aacaccatgc
     2701 ttgagtatgt agctgaaact ttccaccatt tctgtgattc tcattaattc acttgaatct
     2761 ccaaataccc gtagtgcccg atctttcgct agaagcggga tagttttttg aaaaaatatt
     2821 tttgaacaga aaaagctcca atccttatgt ccacattaat tgaatcacaa aaaaatatat
     2881 tacaggaaat ggagctgctt tccgacattc gtggcagccg ggttctggta acgggtgcag
     2941 gagggtttgt tggacttcat ttgtgcgagg cattattgga tttgggcgca gaagtgcatg
     3001 ccttgtcgcg taatattgaa tcgcatgggt taccctatag aattaagacg caccctgtgg
     3061 atttacaaga ttcgcattct ataaaaaaat gcgttgctga tgtgaagcct gaagttgttt
     3121 atcatctggc agggcttgta aacacacggc aggatcttaa tttggtttta ccgacattaa
     3181 gaaacaacct tgtcggatct gtgaacttat ttatggcact agccgaggaa tcatgccgac
     3241 gattggtagt tgtgggttct tcagaagaac ccactgcagg acgacttgga aatgttgcaa
     3301 actcacctta tgccgtttcg aaagaggcgg cgacagaata tgcgagaatg tttcatgaaa
     3361 tattctccct tccagttgtg attacccgcc catacatgag ctatgggccc aggcagccca
     3421 ctgcaaaaat aattccttat gtgatcacaa accttcttgc tggcactcct cccgaagtta
     3481 gcagtggaaa acgaatatgt gatcttattt atgttcaaga tttagtaatg ggtttggtct
     3541 tgtctggttt caagccacaa ttaaccggtg tatcagttga tcttggcact ggagttgctt
     3601 ccactattca tgatgcggtt ggtctgatct cagatatgat gaatgtatca gtaaaaccga
     3661 aatttggagc aataccagat cgtttatatg agacaccaca aattgcgaat agtgaagcaa
     3721 catttcaact gctggggtac cgcccgcaat ggtctttccg tgatggaatt gaactaacag
     3781 ttgaatggta tcgaaaccat ccagaattct ataagacgga aaggtaattt tttatgactt
     3841 ggttgattac cggcggttgt ggttttattg gaagcaactt ggcgaacacc ttgatggagc
     3901 agggagagca ggtgattctg ctggataact taagccgggt tggatcgcaa gataatttgg
     3961 aatggcttcg cagcaggcac ggttcagact gggcttttgt tcagtcagat attcgcgatt
     4021 ctgatgcggt ggaaagaacg ataaagcaac acaagcccaa ggtgattgct cacctggcag
     4081 ggcaagtggc gatgacaacc agtttacaaa atcctcgtat ggattttgag atcaatggta
     4141 tggggacatt gaacgttttg gagtccgtca ggctgcactc attggatacc atcgtgctct
     4201 attcttcaac gaataaagta tatggttctc ttgaagaact tgattatcgt gaaacggatc
     4261 tgcgctatga gcttaccgat catccagatg gactggatga gtcgctccaa ttggatggac
     4321 actccccgta tggctgctct aagttaaccg gagatcaata tgtgcgcgat tacagcagaa
     4381 tgttcgggct gcggaccgtt gtttttcggc attcctcgat gtatggcggc agacaatttg
     4441 caacatatga tcaaggctgg atcggttggt tctgtcaaaa agccctcgaa atggcaaatc
     4501 ctaacagccc gattttcacg atttctggcg atggaaaaca agtccgcgat gtgcttcatg
     4561 cccgagatct ggtaagggta tatttgcagg ctgccaaaca tatcgacagg tcttccgggc
     4621 aaatttataa tatcgggggc gggatgaaga acagcttgtc actattggag ctattcaaac
     4681 tattggaaga aaataccggt ttcccgatgc gatttgaaaa attagaatgg cggcatggcg
     4741 atcaaaaagt atttgtagca aatactgaaa aagcaaaaaa aataacgtgg g
//
LOCUS       DAOQJE010000160         4786 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_10528_length_4786_cov_5.604101, whole genome shotgun sequence.
ACCESSION   DAOQJE010000160 DAOQJE010000000
VERSION     DAOQJE010000160.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 4786)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4786)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4786
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10528_length_4786_cov_5.604101"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..181)
                     /locus_tag="PLT08_05630"
     CDS             complement(<1..181)
                     /locus_tag="PLT08_05630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HNE03976.1"
                     /translation="MKTMATVPTPLPKQEVTRFARFLTVGAVGTLLDFSILTLLKLAG
                     MPTLFANSLSFTAGLL"
     gene            331..1656
                     /locus_tag="PLT08_05635"
     CDS             331..1656
                     /locus_tag="PLT08_05635"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HNE03977.1"
                     /translation="MHSTDDSTRILRTAAWIWLGFLFAMALMDIALYMPQVQNLLGDS
                     TRLQPALPNQPLPPTPIGNLPNSPLRGPHYPVFIFYTANGLTALFFLALTHWSDIQAK
                     MEDIYYPFLLSVISVAPIVINAFIVPRFPQGPLSNAEGMALRQLPVMFVALVLVAWNY
                     RFLHVVLFSIVTTFIELGLVAINPSDNRNITVYIFIAIIRTISFVAVGFFISFIVSRL
                     RKQRESLREANANLTHYASTLEQLTVSRERNRLARELHDTLAHSLTAISVSLETAKAY
                     FDIDLNQSREMIDTSLEATRKGVDETRRALKALRSSDLVDMGLRLAVKKAAESAASRF
                     SLDLELDLQDPMPSLSPDVEQTILRVTQEAIENMTKHSQAKKFSVRLASDKHTTLVIQ
                     DDGIGFDMKPKDSTGHFGLVGMRERAELAGGTLKIESGKGKGTKVVLTI"
     gene            1717..2349
                     /locus_tag="PLT08_05640"
     CDS             1717..2349
                     /locus_tag="PLT08_05640"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HNE03978.1"
                     /translation="MKILLCDDQAVIRDGLEMLLQLEKDFQVIGSAQDGFEAVELASK
                     KSPDLVLMDLKMPGMNGIEATREIRKKHPNMKILVLTTYDDDEWVFDAIRAGASGYLL
                     KDTPRQKIIEAIRGTMDGKSFVDPAVAGKLLNQVANNQTQPASILKDKLTERELDVLR
                     LLAKGMTNTDIAGTLHLSEGTVRNHVSAILEKLGVSDRTQAAVIAIQHGL"
     gene            2416..4140
                     /locus_tag="PLT08_05645"
     CDS             2416..4140
                     /locus_tag="PLT08_05645"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HNE03979.1"
                     /translation="MNRNKITDIFIYIGLMIAFLTPRLPGIGSFVTLDEPQWLSQGAN
                     FYYALGQREFQNTVYEYQPAVTTMEIISLAMFLYFPEYRGLGQGYLDYEKGELDPFMY
                     KHGYDPLVLLAYSRTIQVFVLLILWLILYYLLLRFFTKWVAVFAVLLIAFDPYYLGLT
                     RIMTHEAMVALFILVSLVSLAVYFFRDQNFIFLLISGVAAGFAQLTKSSAIAMLGAIG
                     VLLLFQVFQGQKGNWQKNVLHAFQTFIKWLLILALTYVVFWPGMWVAPGKMLYQVYGN
                     AFSYAFQGARLTVTEDLTPSEFSLNTAPDNTWDVASVLLYRTTPIIWVGGILGLIFLF
                     YSEHGKILNYRVLAAFLLTNTIAFVLLIGLAQGRNSPHYILSSYVSLDVLAAMGWVAL
                     FQRFTFKTTVLSINSMQAIVMAILVVFQVFSSTLKFPYYFTYRNPILYAAGWYQEYPQ
                     KPYGEALEMAADYLSKQPDASDSTAFVYYSRGCFSYFYPGKSISFRPYYIDGDHSEDL
                     MNNLNSSDYLVVYYANQVQFEKYHPFLNILAKSEPYHVIWMDGYEYVRIYKVDTLSSE
                     ILEALAGL"
     gene            4137..>4786
                     /locus_tag="PLT08_05650"
     CDS             4137..>4786
                     /locus_tag="PLT08_05650"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014425.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HNE03980.1"
                     /translation="MKLNKNIWFYILFLVFALPSWLIQNDRVINVDEPRWIVRGANFY
                     YAVTHGEFEKTIFEYHPGVTNMWIVGTAMHIYFPEYRGMGQGYFDPLKTKFEEFIREN
                     DKDVLEIVRISRWLHTGVLAAFFLIALFLLSKLVDIKVAFLSVLLAAVSPFYLGHSRL
                     MNLEGMLAIFVLVSLLGLYIYTNKEKNLFFLVLSGVCFGLAQLTKSSSIVLLGTVG"
BASE COUNT         1199 a          990 c         1082 g         1515 t
ORIGIN      
        1 tgagcagacc cgccgtgaat gagagggagt tcgcaaacaa ggtgggcatt cctgccagtt
       61 tgagcaaagt cagaatgctg aaatctaaca aggtgccaac cgcgccgacg gttaagaagc
      121 gtgcgaagcg ggttacttct tgtttgggga ggggtgtagg aacggttgcc atagttttca
      181 tgttgaatat tttattttcc acccgagaaa gcacagagtg ccccaaatca tgattcctaa
      241 ggatcaccca tgacacctgt catgggtgga tttacaaggt cattacatgg gtcatgccca
      301 tgaaaaatcc cttcatgata agatgcagat atgcattcca cggatgactc cactcgcatt
      361 cttcgcactg ccgcctggat ctggcttggt tttctatttg ccatggcgct gatggatatt
      421 gcgctgtata tgcctcaggt gcaaaatcta ttgggagatt caactcgctt acaacctgcc
      481 ctgccaaacc aacctttgcc gcctaccccc attggaaatt taccaaactc tcctttgcgt
      541 ggaccacact accctgtttt tattttttac actgccaacg ggctaacggc tttatttttc
      601 ctcgctctta cgcattggag tgatattcaa gcaaaaatgg aagatatata ttatccattt
      661 ttgttatcgg ttatatcggt ggcgccgatt gtgatcaacg cttttatcgt tccgcgcttt
      721 ccacaaggtc cgctttccaa tgcagaaggg atggcgttac gtcaattgcc ggtgatgttc
      781 gtagcactgg tgcttgtggc atggaattat cggtttcttc atgtggttct ttttagtatc
      841 gttactactt ttatcgaact tgggctggtc gcaatcaacc catcagataa tcgcaacatt
      901 accgtataca ttttcatcgc cattattcgc accattagtt ttgtagcggt gggtttcttt
      961 atcagtttca ttgtgtcacg tttgcgaaag caacgtgagt cacttcgtga ggcaaatgcc
     1021 aatctcacgc actatgcgtc cacgttggaa cagcttacgg tgagtcgcga gcgtaaccgt
     1081 cttgctcgtg aattgcatga caccctcgca cattccctta ctgccatttc cgtttccctt
     1141 gaaactgcca aagcatattt cgatattgac ctgaatcaat ctcgtgaaat gatcgacaca
     1201 tcgcttgaag cgactcgaaa aggtgtggat gaaacccgcc gcgccttgaa agccttacgt
     1261 tcatccgatt tagtggatat gggcttgagg ctcgccgtga aaaaggcggc agagtccgcc
     1321 gcctctcgct tctctctgga cctcgaactg gatttgcaag accccatgcc ttctctcagc
     1381 ccagacgtag agcaaaccat cctacgtgtg acgcaggaag cgattgagaa catgaccaaa
     1441 cattcacaag caaaaaaatt cagtgtgcgg ttggctagcg ataaacacac gacattagtc
     1501 attcaagatg atggcattgg gtttgatatg aaacccaaag actctacagg tcactttggt
     1561 ttggttggca tgcgcgaacg tgctgagttg gcgggtggca ctctaaaaat cgaaagcgga
     1621 aagggaaaag gcaccaaagt ggtgctgacg atctaattat ttgacgatgc ctatggttac
     1681 ttccatggtc tatcgtccat cgtctacgat ctatttatga aaattctact ttgtgatgat
     1741 caggcagtga tccgcgacgg gttggaaatg cttcttcaat tggagaaaga tttccaagtg
     1801 atcggttctg ctcaagacgg atttgaggca gtggaactcg cttcaaaaaa atctccagac
     1861 cttgttctaa tggacttgaa gatgccgggg atgaacggta ttgaagctac acgtgagatt
     1921 cgtaagaaac acccgaatat gaaaatcctc gtccttacca cttacgatga cgacgagtgg
     1981 gtcttcgatg ccatccgcgc tggggcttcg gggtatttgc tcaaagacac cccacgacaa
     2041 aagatcatcg aagccattcg cggcacgatg gatgggaaat cttttgtcga cccggctgtg
     2101 gcagggaaat tgctcaacca agtcgccaac aatcaaaccc aacctgcctc cattctgaaa
     2161 gacaagctca ccgagcgcga attggacgta ctccgcctgc tcgccaaagg catgaccaac
     2221 accgatattg ccggaaccct gcatctttcc gaaggcacag tccgaaacca cgtcagcgct
     2281 attctggaaa aattaggcgt gtccgatagg acacaagcag cagtcattgc aatacagcat
     2341 gggttataag agagacggtc accattaagg tgaccgtctc tcttcgatgg gggatgtaac
     2401 ggtataatct tggacatgaa tcgtaacaaa ataacggata ttttcatcta cattgggttg
     2461 atgattgcct ttctgactcc gcgtttgccg ggtattgggt cttttgtcac tcttgatgaa
     2521 ccacaatggt tgagtcaggg agcaaacttt tattacgcat taggacagag ggaatttcaa
     2581 aatacggttt atgaatatca gcctgctgtc actacaatgg aaattatctc tctggcgatg
     2641 tttttatact ttccagagta ccgcgggttg ggtcaaggct acctcgatta cgaaaaaggt
     2701 gaattagacc catttatgta taagcatggg tatgatccac tcgttttatt ggcatattca
     2761 cgaaccattc aagtttttgt gttgttgata ttatggctga ttctttatta tctgttgtta
     2821 agattcttta ccaagtgggt agctgttttt gctgttcttc tcatcgcatt tgacccgtat
     2881 tatcttgggc ttactcgcat catgacgcat gaagcaatgg ttgctttgtt tatattggtg
     2941 tctttggtgt ctttggctgt atattttttc agagaccaga attttatttt tttgttgatt
     3001 tctggtgtgg cagcggggtt tgctcagttg acaaaatcat ctgccattgc aatgctaggc
     3061 gccattgggg tattgctttt gtttcaagta tttcaggggc aaaaaggtaa ttggcaaaaa
     3121 aatgtgttgc acgcttttca gacatttatc aagtggctat tgattttggc gctgacttat
     3181 gtagtgtttt ggcccgggat gtgggttgca cctggcaaaa tgctctacca agtttatggg
     3241 aatgccttta gttatgcttt tcagggagct cggttaacag ttactgaaga tttaacccct
     3301 tcagaattta gcctaaatac agcaccagat aacacttggg atgttgctag cgttcttttg
     3361 tatcgaacaa cacccatcat atgggtaggt ggaatattag gattgatttt tctgttttat
     3421 tctgagcatg ggaagatact taattatcgt gtattggcag cttttctgtt aacgaatacg
     3481 attgcttttg ttttgttgat cggcttggcg caagggcgta attcaccaca ttatattttg
     3541 tccagctatg tttctcttga tgtattggca gctatggggt gggtcgcctt gtttcaacga
     3601 tttactttca aaacgactgt cttgtccatc aactctatgc aggcgatagt gatggcaatt
     3661 cttgtcgttt ttcaagtgtt tagttctact ttgaagtttc cttattactt tacatatcga
     3721 aacccaattc tatacgctgc gggctggtat caagaatatc ctcagaaacc ctatggtgag
     3781 gctttggaga tggcagcaga ctatttgtcg aaacagccgg atgcatctga ttcaacagcc
     3841 tttgtctact atagtagagg atgtttttct tatttttacc ctggtaagtc cattagcttt
     3901 cgcccttatt acattgatgg tgatcattct gaagatttga tgaacaactt gaatagctca
     3961 gactatttgg ttgtttatta tgcaaatcaa gttcaatttg aaaaatacca tcctttcttg
     4021 aacattcttg ccaagtctga accttatcat gtgatttgga tggatggcta tgaatacgtc
     4081 cgcatttata aggtggatac cttatcttct gaaatacttg aggctttggc tggtttatga
     4141 aattaaataa gaacatatgg ttctatattc tttttctggt gtttgctctt ccgtcttggc
     4201 taatacaaaa tgaccgggtt attaatgttg atgaacctcg ttggatcgtc cgtggagcta
     4261 atttttacta tgctgttacg catggggagt ttgaaaaaac tatttttgaa tatcatcctg
     4321 gtgttaccaa tatgtggatc gttggtaccg ccatgcacat atattttcct gaatatcgtg
     4381 gtatgggaca aggatatttt gaccccttaa aaacaaagtt tgaagagttt atacgcgaga
     4441 atgataaaga tgtcctggaa attgttcgaa ttagccgttg gcttcataca ggagtattgg
     4501 ctgctttttt cttgattgct ttatttcttt tatcaaaact tgttgatata aaagtagcgt
     4561 ttctatctgt tttattagct gctgtttcac cgttttatct gggtcactcc cgattgatga
     4621 atctggaagg aatgttggct atttttgtgc tggtatccct tttaggattg tatatttata
     4681 caaacaaaga aaagaatctt ttcttccttg tactttctgg ggtttgtttt gggcttgcgc
     4741 aactaacaaa atcgtcttct atagtgcttc ttggaacagt aggtat
//
LOCUS       DAOQJE010000161         4779 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_10552_length_4779_cov_6.517570, whole genome shotgun sequence.
ACCESSION   DAOQJE010000161 DAOQJE010000000
VERSION     DAOQJE010000161.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 4779)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4779)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4779
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10552_length_4779_cov_6.517570"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..298)
                     /locus_tag="PLT08_05655"
     CDS             complement(<1..298)
                     /locus_tag="PLT08_05655"
                     /inference="COORDINATES: protein motif:HMM:NF016001.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase/quinone reductase family protein"
                     /protein_id="HNE03981.1"
                     /translation="MKRPNFIQKFVHRFFMLKPVTAFFATRTHIMDGIALKLTGGKYT
                     VSEILGWNIAQIITTGAKTGEKHTIILIAVINGEKIALIGSNFGRKPNPGWYYNL"
     gene            complement(298..735)
                     /gene="dtd"
                     /locus_tag="PLT08_05660"
     CDS             complement(298..735)
                     /gene="dtd"
                     /locus_tag="PLT08_05660"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015293283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacyl-tRNA deacylase"
                     /protein_id="HNE03982.1"
                     /translation="MRVLIQRVSQASVTVEQQTISSIGKGLLILLGIGHGDGEEQVQF
                     LAEKTANLRIFEDEQGKTNLSVLDVKGEAIVVSQFTLYADSRKGRRPSFIDAALPDVA
                     EPLVHRFVELLRSHGVPTQTGKFGAHMQVEIHNDGPVTIWLEK"
     gene            complement(773..1759)
                     /gene="trpS"
                     /locus_tag="PLT08_05665"
     CDS             complement(773..1759)
                     /gene="trpS"
                     /locus_tag="PLT08_05665"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HNE03983.1"
                     /translation="MAKKGRVFSGARPTGRQHLGNYLGAIENYVGLQTDYDCVYCIVD
                     VHALTTVETTQELRQNTFEMALDWLAAGIRPEDSILFVQSHVPEVMELHTYLSMVTPL
                     GKLTDLPTFKEKVRQQPENVNYGLLGYPVLMTADIVLYKTDIVPVGIDQAPHIEFARE
                     TVRSFNYRFGTKALIEPQVKHTEVKKVVGIDGKDKMSKSLNNHIELALTAEETTKRVR
                     EMVTDPARQRKTDPGNPDVCNVFTMHKIFSPQEEVDMINTECRKAGIGCVDCKLRFAS
                     NLNKHLEPFRAKRADFASKPAEVQEILNDGGKRARAIAQETMSEVREAMKLP"
     gene            complement(1816..2004)
                     /locus_tag="PLT08_05670"
                     /pseudo
     CDS             complement(1816..2004)
                     /locus_tag="PLT08_05670"
                     /inference="COORDINATES: protein motif:HMM:TIGR02174.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Rdx family protein"
     gene            2249..2488
                     /locus_tag="PLT08_05675"
     CDS             2249..2488
                     /locus_tag="PLT08_05675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HNE03984.1"
                     /translation="MPVYTYRCENCGVQFERQQSFTDDPLKTCPECRKKALKKVITPS
                     KVIFKGSGFYATDHKSPSGDSSSSKKSTESSSSAE"
     gene            complement(2550..4073)
                     /locus_tag="PLT08_05680"
     CDS             complement(2550..4073)
                     /locus_tag="PLT08_05680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE03985.1"
                     /translation="MIWITKLKWTDSKIAFLIAMLGSLPLLFYNAFLHDFPMGYAGLF
                     TQMAKQIADASFALPTQSPYYGPGGIPFAYPPLGLYVLAICIKLTGKYYIFLRFLPPL
                     YSLFAVGLTFLLAQKIFKQPFLSMMVAILTATSVDLYVAHTWSSGVVRALAFIFTILV
                     LYFYSPNPDERTRKDIFLAGLFFGLAGLSHLVYALFCFFWIVSTSISFRDWKKTITDP
                     LLAGLTALVTISVWVIPVAVRYGWTVFFGAFNSHGGSDLIGEASNFASLIHLFLINLE
                     PILSNPLLAIWVIVGGVYLLLHKQYHFVAFFLLIVLAFPENARFVYWLGCFFAAYGLW
                     FFANQVYDWGLKKAKFSKPVWMTVLFTLILGILWTIGFNSISRFTPFLSNSAIELQGK
                     SSEIFLKDGTYLALLIQDEAEWLPFLIQHEPLVSQWGSEWLGKYDEQTRRMSLFQGCR
                     KEKDWVCVKAVLAEMNARPEYVITYRIDKKMNEQIAQDGKWREVFSNERYTVWEAEH"
     gene            complement(4070..>4779)
                     /locus_tag="PLT08_05685"
     CDS             complement(4070..>4779)
                     /locus_tag="PLT08_05685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE03986.1"
                     /translation="FISHTWAALLAFILSPKGRFSRFSLFSFVFIVLYAIPISLSDSP
                     RAPAVLMSVYLPSLAVLLAVLIEAIKKYFPSIRLGEWIGIGVFIAVAFPTFYSWAVYD
                     VGIAQAAIAERKVLSVIEQTLRTKGLTSSDEIFSDRYDFYTPNTMPYHARGIEKWSVD
                     WFWGMSETYPLLPNDTWESFATACKEEGIRFLVLGPNSYLRGDFFGQIYTDDVNLDAL
                     GLQFIGQRGNKRLYEFK"
BASE COUNT         1429 a         1142 c         1038 g         1170 t
ORIGIN      
        1 tgaggttgta gtaccagccg gggttgggtt tgcgcccaaa gttggagcca atgagagcaa
       61 ttttttcacc gttgatgaca gcaatcaaaa tgatggtgtg tttttcgcct gtcttcgcgc
      121 cagtggtgat gatctgggca atattccacc ccaaaatttc agaaaccgta tactttccgc
      181 ctgtgagttt aagggcaatc ccatccatga tgtgggtgcg agtggcaaag aaagctgtga
      241 ccggtttcag cataaaaaaa cgatggacaa acttttgaat aaaatttggg cgtttcatta
      301 cttctccaac cagatggtga caggtccatc attatgaatt tcgacctgca tatgggcgcc
      361 gaacttgcct gtttgagtgg gaaccccatg tgaacgtaag agttctacaa agcgatgcac
      421 cagcggttcg gcaacatcgg gcaaagcagc atcgataaaa gaggggcggc gtcccttacg
      481 gctgtcggca tagagcgtga actgtgaaac aacaatcgct tcacctttta catccaaaac
      541 ggagaggttg gtcttacctt gttcatcttc aaagatgcgc aagttggcgg tcttctcagc
      601 aaggaattga acttgctctt ccccatcgcc gtgaccgatg cccaataaaa tcagcagacc
      661 ttttccgatg ctggaaatgg tctgctgttc tacggtgacg ctggcttgag aaactcgttg
      721 gataaggacg cgcataagaa ataaataggg cggggaaacc ccgcccctgc gtttaaggta
      781 acttcatcgc ctcgcggact tcgctcatgg tttcttgagc aatggcgcgg gcgcgtttgc
      841 cgccatcgtt gaggatttcc tgcacttcgg caggtttaga agcaaagtcg gcgcgtttgg
      901 cgcggaaggg ttcaagatgt ttgttgaggt tagaagcgaa gcgcagtttg caatccacac
      961 agccaatgcc agctttacgg cattcggtat tgatcatgtc tacttcttct tgcggtgaga
     1021 agatcttgtg catggtgaac acgttgcata catcagggtt gccggggtct gtcttgcgct
     1081 gacgagccgg gtctgtgacc atctcgcgca cacgtttggt ggtctcttcg gcagtgagcg
     1141 ccaactcaat atgattattg aggctcttgc tcatcttatc cttgccgtcg ataccgacca
     1201 cttttttcac ttcagtgtgc ttgacctgtg gttcaatcaa ggcttttgtg ccaaaacgat
     1261 aattgaacga gcggacggtc tcgcgcgcaa attcgatatg cggagcctgg tcaatgccga
     1321 ccgggacaat atccgtctta tacaaaacaa tgtccgctgt catcaaaacc gggtaaccca
     1381 gcaaaccata gttgacattt tctggctgtt ggcgaacttt ctctttgaag gttggcaaat
     1441 cggtcaactt accaagcgga gtgaccatgg aaagataggt gtgtaattcc atcacttcag
     1501 gcacgtgtga ttgcacaaac aagatcgaat cttccggtcg aatccctgct gcgagccagt
     1561 cgagcgccat ttcaaaggtg ttctgacgaa gctcctgtgt cgtttccaca gtggtcaatg
     1621 catgaacatc cacgatgcaa tacacacaat catagtcagt ttgtaagcct acataattct
     1681 caattgcgcc aaggtaattc cccaaatgtt ggcgtcccgt tgggcgcgcg ccagaaaata
     1741 ctcgtccttt ttttgccatt cgtatgtcct tgttatccgc aagggagacc tagttgggtc
     1801 acccctacat gtaatttaat caaccatctt agatattaga ccaagcactt cgcctgcttc
     1861 tgcatgacgc ttggtctgca atttggagta gaccaactcc ccgttcacgc tgacctcgaa
     1921 cctgcctccg tccgaaggaa caagagtgat cccctcgatg acgtgctcat aatctttcaa
     1981 caattcctgt gccagactca cggcccggtt ggtgtaatgt caaaccgcac aatagacgat
     2041 ctcaatcttt agcatagttg ctccacaaga ccttcaccct atagactgca acttttcttg
     2101 tcccctcaca tctaacaagg tgccaggtca gcgaaattgt aagaaaatta taacatgctg
     2161 cttctgaagg cttgccacac cctaacaagg gggctataat gccgcagttg tatccaagaa
     2221 atattagtaa gaagcaagga gagaacgtat gcccgtctac acttatcgat gcgagaattg
     2281 cggagtgcaa ttcgaacgcc agcaatcctt caccgatgat ccattgaaga cctgccccga
     2341 gtgccgcaag aaagcactga aaaaggtcat cacccctagc aaagtgattt tcaagggttc
     2401 agggttctat gccaccgacc acaagtcacc ttctggagat tcctcttctt ccaagaagag
     2461 caccgaaagc tcctcctctg ctgaataagt agagtctagt acggcatatc aagaaggtca
     2521 cactcatggg tgtgaccttc ttttttattt taatgctctg cttcccaaac cgtatacctt
     2581 tcgttcgaga aaacctctcg ccacttacca tcttgggcaa tctgttcgtt cattttttta
     2641 tcaatacggt aagtgataac atattcaggt ctagcattca tctctgccaa cacagctttt
     2701 acacacaccc aatctttttc ttttcgacac ccttggaata gtgacatccg acgagtctgc
     2761 tcatcgtact tgcccagcca ttcacttccc cattgggata ccaacggctc atgctggatg
     2821 agaaatggta accactcagc ttcatcctgt atcaatagtg ccaagtacgt cccatccttc
     2881 aaaaatattt cagaggattt tccctgaagc tctatagccg agttacttaa gaacggagta
     2941 aatcgcgaaa tagaattaaa accaatcgtc caaagaattc ctaaaatcag agtaaacagc
     3001 actgtcatcc aaacaggctt agaaaatttg gcttttttca atccccaatc gtatacctga
     3061 ttagcaaaga accacaatcc ataggcggca aaaaagcacc caagccaata tacaaaacgg
     3121 gcattttcgg ggaaagccaa aacaataagc aaaaagaagg caacaaaatg atattgtttg
     3181 tgaagaagca aataaacccc gccaacaatc acccagatcg ccaaaagtgg atttgacaga
     3241 attggctcga gattaatcag gaacagatga ataaggctgg cgaagtttga tgcctctcct
     3301 atcaaatcac tccccccatg agaattgaag gctccgaaga aaaccgtcca cccataacga
     3361 actgcaacag ggatgaccca aacactgata gtaaccagcg ccgtcaaacc agccagtaat
     3421 ggatctgtga ttgtcttctt ccaatcacga aatgaaattg aggtgctcac gatccaaaag
     3481 aagcaaaaca atgcatatac cagatggctc agcccagcta gcccaaaaaa cagccccgct
     3541 aaaaagatat ctttccgcgt ccgttcatca ggattaggag agtagaaata caaaaccaag
     3601 atcgtaaaaa taaaagcaag ggcacgcaca acacccgaag accaagtatg tgctacatat
     3661 aaatccactg aggttgcagt caagatcgcg accatcatcg aaaggaaagg ctgtttgaaa
     3721 attttttgag cgaggagaaa ggtcaaccca accgcgaaga gactataaag tggtgggaga
     3781 aagcgaagga agatgtaata cttacccgtc aacttgatac atatagcaag tacatacaag
     3841 cccaatggtg gataggcaaa tggaatgcca cctggaccat aatatggaga ttgagtgggc
     3901 aaagcaaagc tcgcgtccgc aatttgtttt gccatttgtg taaacaaccc agcatacccc
     3961 attgggaagt catgtaaaaa tgcgttataa aaaagtaaag gaagagaacc caacattgca
     4021 atcaaaaaag caattttgga atcagtccat tttaattttg taatccagat catttgaact
     4081 cgtacaatct tttattgccc ctttgcccaa taaattgcaa gcctaaagca tcaaggttaa
     4141 catcatcggt atagatttga ccaaagaaat ctccacgaag atagctattc gggccaagaa
     4201 cgagaaaacg aattccctct tctttacaag cagtggcaaa tgattcccaa gtgtcatttg
     4261 gcaataatgg atatgtttca ctcattcccc agaaccaatc gacggaccat ttttcgatcc
     4321 ctctagcatg ataaggcatg gtattcgggg tataaaaatc gtaccgatca gagaaaattt
     4381 catcagaaga agtcagacct tttgtgcgaa gagtctgttc aataacagaa agaactttcc
     4441 gttcggcaat ggcagcctgt gctataccga catcatatac agcccagcta taaaatgttg
     4501 gaaaggcgac agcaataaat actccaattc caatccattc acccaaacga atagagggaa
     4561 aatatttctt aatagcttcg atcaaaacgg ctaacaaaac ggctaacgat gggagataca
     4621 cgctcatcaa aacagctggt gcacgagggc tatcgctaag cgagatcgga attgcataca
     4681 aaacaataaa aacgaaggaa aataaagaaa agcgggagaa acgccctttt ggcgaaagga
     4741 taaatgcaag caaagcagcc caagtatggg aaatgaaat
//
LOCUS       DAOQJE010000162         4770 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_10582_length_4770_cov_5.786426, whole genome shotgun sequence.
ACCESSION   DAOQJE010000162 DAOQJE010000000
VERSION     DAOQJE010000162.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 4770)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4770)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4770
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10582_length_4770_cov_5.786426"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1136)
                     /locus_tag="PLT08_05690"
     CDS             complement(<1..1136)
                     /locus_tag="PLT08_05690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase family protein"
                     /protein_id="HNE03987.1"
                     /translation="MIYLDYAATTPVDERVLAKMMPYFRDSFGNPSSIHRFGQKAEAA
                     VDEAREKVASVLGCRPDEVIFTSGGSEADNLALRGAMVANKASTKWLLTAKTEHPAVS
                     KTAIQLEKEYGFPLEWLDVNEHGVVTVESLSKAICNDTTMASVMYGNNEIGTINPIAD
                     LAKVAKSNNVLFHTDAVQAAAYLDVNVNKLGVDLMSLGGHKFYGPKGVGALYVRKGTK
                     LVPHLTGGSQESSLRAGTHNVPYIVGFSEALSLAAEEREARIAHVKPMRDHIIGTVLE
                     TISDAKLTGHPEQRLPNHASFVFKDVDGNLLLQMLDAAGFACSSGSACKTGNPEPSDV
                     VTALGHSHDWALGSLRITLGTGTTPEHVESFLQALPSLVEKTRS"
     gene            1199..2836
                     /locus_tag="PLT08_05695"
     CDS             1199..2836
                     /locus_tag="PLT08_05695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE03988.1"
                     /translation="MHNRTQWLKIVGGALGATAVLLMVFANQLGLESFPTWGVRRVTI
                     LLIGILLLSVSLFYQEDNFIGKFIHSADGPLYIALLVLNAGIFLIYIWYVSTGLMKTF
                     TSETNYFDLQANAFAKGQLALDVQPDPALLAFKDESLYEPENRIGIPVLWDATLYQGK
                     YYLYWGPAPALLLAAIKAFYTPDIGDRLLVLIFLTGTLVFLNLIILDLRKRYFPNIPN
                     WAVLVAVAFAGMINPMLYVLVEGRIYEAAIVAAQFFLIGGFYFLLPTFEKPTFPRLAL
                     AGLFFVFSIGSRTTTLPGIALISITLLLWAYQTQRPRMFPILLAFALPVAIGGIAYAT
                     YNYARFGSFTEFGYRYQLTSYNLYQKMDETFAIEYIPPNLYKTLFNPLETRDAFPRLF
                     PTRWSGPEWLTNYHPKMYLTLTENITGVFVSSPFVLLALLAFFKPRRDLRYVITSITL
                     CFLGIFITLQAFFFVAMRYMLDVIPTLSLLVIIGFWHGFELFKNKKVYIIITTLLLIH
                     TIACSLLISFSGNLETFKILNPELVREMTWTFNNLLK"
     gene            2938..3966
                     /locus_tag="PLT08_05700"
     CDS             2938..3966
                     /locus_tag="PLT08_05700"
                     /inference="COORDINATES: protein motif:HMM:NF024245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HNE03989.1"
                     /translation="MSRFLDFLNTADLNTLTQVSGVNRQLAGNLIAARPFDAEEDALN
                     VKGMGKATLARLQSFAEAQGNGSESSAMVPVEEEAMPAVMEKNPPAQEDAPKKESFFV
                     RLGRAIRSFFVALLKLIMLAILFVSIGAMFYYGLPYIQRTFIAPVEQNTAQIKTMEAE
                     IETLQTQLDEMNSRVSALEGSVEAHTASIEKLEEMQTALETQLEENQDAVLLELKQEI
                     MFTRALDVLARARLYLAQSNFGLAKTDVQTARDLLAELNAEKEDEVLTKAIERIDLAL
                     DNLPDFPVVASGDLEIAWQILVSGNAPASTATATPTLVPTEPATTTPTTVDTAATPTL
                     IPSATATP"
     gene            4065..>4770
                     /locus_tag="PLT08_05705"
     CDS             4065..>4770
                     /locus_tag="PLT08_05705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucomutase/phosphomannomutase family
                     protein"
                     /protein_id="HNE03990.1"
                     /translation="MAYKIKFGTDGWRGVIAEDYTFDNLRRCAQGFALYMLSQGKKGQ
                     WVVVGHDKRFHSENFAAAVAEVLAGNGLKVYLTDGATPTPVIAYAVVDKKAAGAVNIT
                     ASHNPPTDNGFKVRNETGGAIDPEGLKQIEAGIPDDLKDVKLKNYKEAEAAGEIVKFD
                     AATPYIEHLTRDGLIDLQPIKDAGLTVVVDAMWGNGAGWFTRLLGGGKTKIIEIHNER
                     NPSFPEMKRPEPIRPNI"
BASE COUNT         1196 a         1222 c         1087 g         1265 t
ORIGIN      
        1 aaacttcttg tcttctctac aagcgaaggc aaagcctgca agaaagattc gacgtgttca
       61 ggggttgtcc cagttccgag cgtaatgcgc agcgagccta atgcccaatc atgggaatgc
      121 cccaaagcag tgaccacatc actgggttcg ggattccccg tcttacacgc cgagccagaa
      181 gaacaagcaa acccggcagc atcgagcatc tgcaaaagca aattaccatc tacatctttg
      241 aatacaaaag aagcatggtt agggagacgt tgttcgggat gaccagtaag ttttgcatca
      301 gagattgttt caagcacggt accaataata tggtctcgca taggttttac atgggcaatt
      361 cgcgcttcac gttcttccgc agctagagac aatgcctcgg aaaatccaac gatatacggc
      421 acattatgcg tccccgcccg caagctagac tcctggcttc cgcccgtaag atgtggcacc
      481 aacttcgtcc ctttgcggac gtacaacgcc cccacccctt tcggtccgta gaatttatgt
      541 ccccccaacg acatcaagtc cacaccgagt ttgttcacat ttacatcgag atatgccgcg
      601 gcttggacag catctgtatg gaataagaca ttattcgact tggcaacttt tgccaagtct
      661 gcaatcgggt tgatggtgcc aatttcatta tttccataca tcacagacgc catcgtcgtg
      721 tcattgcaaa tcgcctttga aagcgattcg accgtcacaa caccatgttc atttacatcc
      781 aaccattcca gcggaaaacc atattccttt tcaagttgta tcgccgtttt gcttacagcg
      841 gggtgttcgg tcttggcagt tagcaaccac ttggtcgagg ctttattcgc caccatggcg
      901 ccgcgcaagg cgaggttatc cgcttctgag ccgccagagg tgaaaatgac ctcatcgggg
      961 cggcatccta aaaccgaggc aaccttctcg cgcgcctcat ccactgcggc ttccgctttt
     1021 tgcccaaaac ggtgaattga cgaggggttt ccaaacgaat cgcggaaata tggcatcatt
     1081 ttagcgagca cacgctcgtc tacgggtgtg gtcgcggcat aatcaagata aatcatgact
     1141 gggattatag caaaggttaa tcataaaaag accggtctca aaatgataaa atcattccat
     1201 gcacaatcgt actcaatggc taaaaatcgt aggtggggca ctcggagcta ccgctgttct
     1261 tttgatggtc tttgcaaatc aattgggatt agaaagcttc cctacttggg gggtacgtcg
     1321 cgtcacaatt ttattgatcg gcattcttct gctctcagtt tctcttttct atcaagaaga
     1381 taatttcatc ggcaagttca ttcattcagc agatggtccg ctttatattg cgcttcttgt
     1441 tctcaatgca ggcatctttc tcatttacat ttggtacgtc agcaccgggc tgatgaaaac
     1501 ctttaccagc gaaaccaatt attttgacct gcaagccaat gcttttgcca aaggtcaact
     1561 ggcgttggat gttcagcccg accctgctct cttggcattc aaagacgaaa gcctatacga
     1621 gccagaaaat cgcatcggca ttccagtctt atgggatgcg actctctatc aaggcaaata
     1681 ctatttatat tggggtccgg cacctgcgct tttattagct gccatcaaag ccttttacac
     1741 acccgatatt ggcgaccgtc tgctagtgtt gattttcctc actggcacct tggtcttcct
     1801 taacttgatt attctcgacc tgcgaaaaag gtattttcct aacattccaa attgggcagt
     1861 tcttgttgcg gttgcatttg cagggatgat caacccgatg ttgtatgtgt tggtggaagg
     1921 gcgcatttac gaagctgcta tcgtggcggc gcaattcttt ctgattggcg ggttttattt
     1981 tcttttgccc acatttgaaa aaccaacttt tccgcggttg gcgctggctg gcttgttctt
     2041 cgtcttttcg attgggtcgc gcacaacaac attacccggt attgctctca tctctattac
     2101 acttctattg tgggcatatc aaactcaacg tccacgcatg tttccgattt tgctggcatt
     2161 tgcccttccc gttgccatag gcggcatagc atatgccacg tataactatg ctcgtttcgg
     2221 ttctttcacc gaatttggct atcgttacca actcaccagc tataacctgt atcaaaagat
     2281 ggatgagacc ttcgccattg aatatattcc gcccaattta tataaaaccc ttttcaaccc
     2341 gctcgaaaca agagatgcat ttccacgcct cttccctact cgttggtcgg gtcctgaatg
     2401 gctgacgaat tatcacccca agatgtattt aaccctcacc gaaaacatca ccggtgtatt
     2461 cgtgagcagt ccattcgttt tgctggcttt gctggcgttt ttcaaaccac ggcgagactt
     2521 acgctatgtc attacatcca tcaccttatg ttttcttggc atcttcatca ccttacaagc
     2581 cttcttcttc gtcgccatgc gctacatgtt ggatgtcatt ccgacactat cccttctcgt
     2641 tatcattggc ttttggcatg gctttgaact tttcaagaac aagaaagtgt atatcatcat
     2701 cactacgctt ttattgattc ataccatcgc atgtagtttg ttgatttcgt tctcaggcaa
     2761 tttggaaacc ttcaagattc tcaacccaga actcgtccgc gagatgacat ggactttcaa
     2821 caaccttctc aaataacaga tatccacgcc tcgtgcgtga cgtgggcttg catctattac
     2881 ctcaagctga ttcgcatctg aaaccttttg ttgtacaatg accgtatagg agattccatg
     2941 agccgctttc tcgattttct caacactgca gacctgaata ccctcactca agtatctggc
     3001 gttaaccgtc aactggcagg gaatctcatt gccgcgcgtc ctttcgatgc agaagaggat
     3061 gcgctcaacg ttaaaggcat gggcaaagca acccttgcgc gcttgcagtc ttttgctgag
     3121 gcgcagggaa atggttcaga aagcagtgcc atggttccgg tcgaggaaga ggcgatgccc
     3181 gccgtaatgg agaagaatcc gcctgcacag gaagatgccc cgaagaaaga atcattcttc
     3241 gtgcgtttag ggcgggcaat tcgatccttc tttgtagcat tgttaaagct gatcatgttg
     3301 gcgatcttgt tcgtatcgat tggcgcaatg ttctattatg gcttgccata cattcagcgg
     3361 accttcattg cgcctgtaga acaaaacacg gcacaaatta aaaccatgga agcagagatc
     3421 gaaacgctcc aaactcagct cgatgaaatg aactcacgcg tttctgcact tgagggctcg
     3481 gtagaagctc atacggcttc gattgaaaaa ctggaagaga tgcaaaccgc gctggaaact
     3541 caactcgaag aaaatcagga tgcggttctg ttggaactga aacaagagat catgttcacc
     3601 cgtgcgttgg atgttctagc acgtgcacgg ctgtacttgg cacaaagcaa ttttggcttg
     3661 gcaaagaccg atgtgcaaac cgcacgagat ttgctggctg aactcaatgc cgaaaaagaa
     3721 gatgaggttc taaccaaagc catcgagcgg attgaccttg cgcttgacaa cctgccagac
     3781 ttccctgtcg ttgcctcagg agatttggaa attgcctggc agattttggt ctctgggaat
     3841 gcccccgcat ccactgcaac ggcgacaccc acgctagtcc ccacagagcc agcgaccacc
     3901 acccccacaa cagtggacac ggcagctacg cctacattaa ttccatctgc aacagcaacc
     3961 ccgtaacgca aaactccgag gcaatgcctc ggagtttttt tatgatattt aggctcgtgg
     4021 cgggtataat ttcgaaagtt atcattccca agaggagaat tctcatggca tacaaaatca
     4081 aattcggcac agacggttgg cgcggagtca ttgctgaaga ttacacgttc gacaacctgc
     4141 gtcgctgtgc gcaggggttt gcgttgtata tgctttcaca aggtaaaaag ggacagtggg
     4201 tcgtagttgg gcatgacaag cgtttccact ccgaaaattt tgctgcagct gtggcagaag
     4261 tgttggctgg caacggcttg aaagtctatc tgaccgatgg cgcgacccca accccggtca
     4321 ttgcttatgc cgtggtggat aaaaaagctg ccggtgcggt gaacatcaca gccagccaca
     4381 acccacccac cgataatggc ttcaaggtcc gcaatgagac cggtggcgct attgaccctg
     4441 aaggtttgaa acaaatcgaa gcaggcattc ctgatgattt gaaggatgta aagctcaaaa
     4501 attataaaga agccgaagcc gctggcgaga tcgtcaagtt cgatgcggcg actccgtata
     4561 ttgaacacct cactcgcgat ggattgatcg atttacagcc catcaaagat gccggtctga
     4621 ctgtggtggt ggatgccatg tggggcaatg gtgcaggttg gttcacccgt ctgctcggcg
     4681 gcggtaagac caagatcatt gaaattcaca atgaacgcaa cccttccttc cctgaaatga
     4741 agcgcccgga acccatccgc ccgaacattg
//
LOCUS       DAOQJE010000163         4762 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_10613_length_4762_cov_7.222860, whole genome shotgun sequence.
ACCESSION   DAOQJE010000163 DAOQJE010000000
VERSION     DAOQJE010000163.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 4762)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4762)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4762
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10613_length_4762_cov_7.222860"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            68..1924
                     /locus_tag="PLT08_05710"
     CDS             68..1924
                     /locus_tag="PLT08_05710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE03991.1"
                     /translation="MISSKLFKKELPSWLPLVLAGVGAILYLIQAIVYAHTTVSSLDE
                     GSYLIKGMFYLNGVYKPFEPYGPLTNKAPFAFLIPGVAEYIFGAGLRTGRYFSIFLSL
                     LTILGVWIVSRRWSGNWFAAFTVYFFVLSPMIIKLYALAVSEVIIACILAWVCVLVLA
                     EERPLWQIILASFLAAAAVFARQNVVPILPFMILYIFWQHGKSKGIWSFGTTSIFFLL
                     FHIIYWPRVMTIWAPWLPESLTPFLNSFRVPTDSVPIWDPTIDFWGRLNSFFQGIRYH
                     FIPLVGGFFGLLLIPPRAEWKSASAIRAAVFLAVSYFVLAAMHGWAAIASQYESYSCV
                     FCFSNYLGFFDPLGILFFIIIFSYAWNRNPNSVVQVVIVFSALVIATGIGYSLFEKVG
                     NGLLNTPVIPRLRSSGLGFVTIFDFLTHGFDMPLVQIKRWISASLGFIIGIAVIFIAF
                     FIWKKRNAPRFAHVLANTFLISGLILSPILHLGGSQIDCQTDIIRDHEGLGTYLASII
                     PAGSLVYWDGGNAYTPMIYVPEARIFPPQINDGYTFHIGGDPDVLYYFSHWNAELDAR
                     WRAEADVFIIEAKRYANWKDFLTPQEFQEFAKPSVSPSCVEGAELRIFQRLP"
     gene            1921..2877
                     /locus_tag="PLT08_05715"
     CDS             1921..2877
                     /locus_tag="PLT08_05715"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HNE03992.1"
                     /translation="MNLSLIIPVYNEQDNLPMLFEALYATMNSLGKTWEVILVDDGSR
                     DKSLSILEEYAAKDADHVRVISFRRNFGQTAAIAAGLDYSQGDIIILLDADMQNDPSD
                     IPMLLAKLDEGYDLVSGWRKQRKDNAITRNLPSMMANKLISMVTGVELHDYGCTLKAY
                     RRPVLEGFRLYGEMHRFIPVFASSVGARITEVVVNHHPRRFGKTKYGLERTVKVVLDL
                     FTVKFLVSFSSKPIYLFGGTGLGLMVISALTMLYLFIRRIFFLVGVTGSPLFQTSTML
                     FILGFQSMLMGLIAELLVRTYHESQRKTTYTVRTKINIKDND"
     gene            2870..3787
                     /locus_tag="PLT08_05720"
     CDS             2870..3787
                     /locus_tag="PLT08_05720"
                     /inference="COORDINATES: protein motif:HMM:NF015651.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase transmembrane
                     domain-containing protein"
                     /protein_id="HNE03993.1"
                     /translation="MIKWLRENRQMVARGLGSLLALALLLVLIQEEGDGELFSAIRRV
                     SLVYFLLALLALLISRTFAATRWFVLLRSAGVKIPLSRAIMLMFTGNFSSNFLPTTIG
                     GDVVRLGGAMQMGYDRAICLASLVVDRLIGMAGMALALPLGLIPLFSLNSSLVQSFTL
                     AGLFQKGVDFGKRTFEAFSIWLKKPLGLSGALLSTLGNQVFIYLMIYLLLQGIDHHVS
                     FWLVAGMYTLTYFVTLVPISINGLGVQELSMTFLLVQLGGLTSSESATIALLTRLLFI
                     LTSLPGAFFLPSILAAMNEKKVEIPESNG"
     gene            3794..>4762
                     /locus_tag="PLT08_05725"
     CDS             3794..>4762
                     /locus_tag="PLT08_05725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE03994.1"
                     /translation="MESVSTRKFLQVWLWALAVVSLCAILQTIQRTIELEIVLLRSKW
                     IGLVGIFAFTAILSVWVSFSSFLNHIVSWFDNLENQSLSPLRITFYVALIIFGFLVSI
                     FFRLYIFSNTLPQVMPILWIFLWASLAQTIGLKQLRKFDWASSFAIIVLIQGLIYQTY
                     EIFAITSANPFSMGYSEAGRHYYASLFFSEKLYGMELPLPFLHPSRYLLLSIPFLIDG
                     LPLWFHRFWQAFLWFGLTLGASLSLARFSRTSGWKYIALTAWAFLFFFQGAVYYHLHV
                     MVILILVGVSVNKPWRSLVFIILASLWAGISRVNWFPVPAMLAIAIY"
BASE COUNT         1037 a         1046 c         1067 g         1612 t
ORIGIN      
        1 ccataaaaaa ttaggttaat gaattaggtg acagttgctc aataagtgac tgtcacttgc
       61 tttatttatg atttcttcaa aattgttcaa aaaagaattg ccctcttggt tgccgctcgt
      121 tctggcaggt gtgggggcga ttctgtattt aatccaagca attgtctacg ctcatacaac
      181 tgtcagcagc ttggatgaag gttcctacct catcaaaggc atgttctatt tgaacggggt
      241 ttacaaaccg ttcgagcctt atggtccgct gactaacaaa gctccatttg catttctgat
      301 tccgggtgtt gctgaatata tttttggcgc ggggttacgc accggaagat atttttcaat
      361 attcttgagc ttattaacta ttttgggagt ttggattgtg tcgcgccgtt ggtcaggcaa
      421 ttggtttgcg gcatttactg tttatttctt tgtacttagc ccaatgatca ttaagctcta
      481 tgctcttgcc gtctcagaag tgatcattgc ctgtattctg gcttgggttt gtgtcttggt
      541 attggcagaa gaacgcccac tttggcagat tattcttgca tcatttttgg cggctgcggc
      601 agtatttgcc cgtcaaaatg tggtgccgat attacccttt atgatcttat acatcttttg
      661 gcaacatgga aagtcaaaag gtatatggtc atttggtaca acatccatat tctttctatt
      721 gtttcatatt atttactggc cgcgtgtaat gaccatttgg gcgccgtggt tgcctgaaag
      781 tctcacacct tttctgaatt cttttcgtgt gccaacagac tccgtcccga tctgggatcc
      841 tactatcgat ttttggggac gacttaattc tttcttccag ggaatccgat accatttcat
      901 tccgttggtt ggcggtttct tcggactttt attaattcca cctcgcgccg agtggaagtc
      961 tgcttctgca attcgagcgg ctgtttttct cgctgtgagt tattttgtgt tggctgctat
     1021 gcacggttgg gcggcaatag caagccaata tgaaagctat tcctgtgtgt tttgtttttc
     1081 caactatctc ggcttctttg accctttagg gattttattt ttcatcataa tcttttcata
     1141 tgcttggaat cgaaatccta attctgttgt ccaggtcgta attgttttta gcgcattggt
     1201 aatcgccacg ggtataggct attctttgtt tgagaaagtc ggtaatgggc tcttaaacac
     1261 cccagtgatt ccgcgccttc gttctagtgg acttggcttt gtaacgattt ttgacttttt
     1321 gacgcatggc tttgatatgc cgcttgtaca gatcaagcgc tggattagtg cttctcttgg
     1381 ctttattata ggaatagctg ttatttttat tgctttcttc atttggaaga agcgaaatgc
     1441 tccacgattt gcccatgtgc ttgcgaatac attcttaatc agcgggctta tcctttctcc
     1501 cattctccac cttggtggaa gccaaataga ctgtcagacc gacatcatcc gtgaccatga
     1561 ggggttggga acttatcttg cttcgatcat tccggctggg agtctggtat attgggacgg
     1621 cggcaacgca tacaccccaa tgatctatgt gccagaggcg cgtattttcc cgccgcagat
     1681 caatgatggg tatactttcc acattggtgg cgacccagat gtactgtact atttcagtca
     1741 ctggaatgcc gaattggatg cccgttggcg tgccgaagcg gatgttttca tcattgaagc
     1801 caaacgctac gcaaattgga aagatttctt aactcctcag gagttccaag agtttgcaaa
     1861 accctctgta tctccttctt gtgttgaagg tgcagaacta aggatatttc aacgattacc
     1921 atgaaccttt ctctcattat tcctgtttac aacgaacagg ataacctccc aatgcttttt
     1981 gaagctcttt atgcaacaat gaactccctt ggcaagacgt gggaagttat tcttgtagat
     2041 gatggcagcc gtgataaaag tctttctatt cttgaggaat acgctgcaaa agacgcagat
     2101 cacgtccgtg tgatttcatt ccgtcgcaat tttgggcaga cagctgccat tgccgctggt
     2161 ttggattatt ctcaaggtga catcatcatt ctgttggatg cagatatgca gaatgaccct
     2221 tctgatatcc ccatgctttt agcaaaactt gatgaagggt atgacctcgt cagcggctgg
     2281 cgtaaacaac gtaaagacaa tgccataacg cgtaacctcc cctctatgat ggcgaataag
     2341 ctcatttcta tggttacggg tgtggagttg catgattatg gttgcactct aaaagcttat
     2401 cgtcgcccgg tcttggaagg ttttcgttta tacggcgaaa tgcatcgctt cattccagtc
     2461 tttgccagct cggttggagc acgaatcaca gaagtcgttg tcaaccacca cccacgccga
     2521 tttggaaaga ccaagtatgg gctggaaaga accgtgaagg tcgttctcga tttgtttacg
     2581 gtgaaattct tggtcagttt ttcatcaaaa cccatttatc tgtttggtgg aactggtttg
     2641 gggttgatgg ttattagcgc tctgactatg ttgtacttgt tcattcgtcg cattttcttc
     2701 cttgtcggtg ttacgggttc gccgctattt caaacaagta ccatgttgtt catccttggt
     2761 ttccaatcca tgttgatggg tttgatcgcg gaacttctag ttcgcaccta tcacgagtcg
     2821 cagcgtaaga cgacgtatac agttcgtacg aagatcaata tcaaagataa tgattaagtg
     2881 gttgcgcgaa aatcgccaga tggttgcccg tggattaggc agccttctgg cattggcttt
     2941 acttttagta ctaatccaag aagaggggga tggagaatta ttttcggcga ttcgccgggt
     3001 ttcactagta tattttttat tggcacttct cgctttatta atctctcgca catttgcagc
     3061 aacccgttgg tttgtcttgc ttcgttcggc tggggtaaag ataccgttat cgcgtgccat
     3121 tatgctcatg ttcaccggca acttctcatc caatttttta ccaacgacga ttggtggaga
     3181 tgttgtgcgc ttgggcggcg ccatgcaaat gggctatgac cgtgcaatct gtctcgcctc
     3241 gctcgtagtg gaccgtttga ttgggatggc aggaatggca ttggcactcc ctttaggttt
     3301 gattccccta ttttctttga atagtagttt ggttcagtct tttacattgg caggtttatt
     3361 tcaaaaaggg gtggattttg gcaaacgcac ctttgaagcc ttctccatct ggttgaagaa
     3421 acccttagga ctaagcgggg cattattatc cacacttggc aaccaagtat ttatttattt
     3481 gatgatctac ctgctcttgc aaggcattga ccatcacgtc tctttctggc ttgtagctgg
     3541 gatgtacacc ctgacctatt tcgtcaccct cgtgccgatc tctattaatg ggcttggtgt
     3601 gcaggaactt tcaatgactt ttctgcttgt ccaacttggc ggattaacgt cttctgaaag
     3661 cgcgaccatt gctttgctta cccgtctctt gttcatcctc actagtctgc ctggggcttt
     3721 cttcttgcca tccattttgg cggcgatgaa cgagaaaaaa gttgaaatac cagaatcgaa
     3781 cggatgaaga aatatggaat ccgtttctac gcgaaaattt cttcaagttt ggctatgggc
     3841 tctggcggtt gttagcctgt gtgcgatttt acaaaccatt caacgcacaa ttgaattaga
     3901 gatcgttctg ctccgctcca agtggattgg cttggtaggc atctttgcat ttacagccat
     3961 tttgtctgta tgggtatctt tctcttcctt tttgaaccat attgtttctt ggttcgataa
     4021 cctcgaaaac caatccttat ctcccttaag aattaccttt tatgttgcac tcatcatttt
     4081 cggctttctt gtaagtatat tcttccggct atatattttt agcaataccc tgccccaagt
     4141 tatgcccatc ctatggatat tcctatgggc aagccttgcg cagactattg gattgaaaca
     4201 actccgcaaa tttgattggg cttcttcatt tgcaattatt gttttaattc aaggcttgat
     4261 ctatcaaacc tacgaaatct ttgccattac ttctgctaat cctttttcga tgggttactc
     4321 cgaggcggga cgacattatt atgcttcgct atttttctct gaaaaattgt atggaatgga
     4381 attgccgctt ccattccttc acccgtcacg gtatttgctc ctctccatcc cgttcctaat
     4441 tgacggattg ccgctttggt tccatcgttt ttggcaggca ttcttatggt ttgggttgac
     4501 gcttggcgcg tctctgagtt tggctcgttt ctctcgcaca agcggatgga aatatattgc
     4561 tctcactgct tgggcttttc tgttcttctt ccaaggcgca gtctattatc acttgcatgt
     4621 aatggtgatt ctgattcttg taggtgtttc ggtcaataaa ccgtggcgct cgcttgtatt
     4681 cattatcctt gcttcgcttt gggcagggat cagccgagtg aactggttcc ctgtcccggc
     4741 gatgttggca atagcgatct at
//
LOCUS       DAOQJE010000164         4753 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_10642_length_4753_cov_5.573436, whole genome shotgun sequence.
ACCESSION   DAOQJE010000164 DAOQJE010000000
VERSION     DAOQJE010000164.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 4753)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4753)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4753
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10642_length_4753_cov_5.573436"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            129..1469
                     /locus_tag="PLT08_05730"
     CDS             129..1469
                     /locus_tag="PLT08_05730"
                     /inference="COORDINATES: protein motif:HMM:NF024241.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNE03995.1"
                     /translation="MTETTQTPPEEKLDFKRILPILVIVLVDLMGLSIIIPLLPLYAA
                     RFGAEPIVVGILQATYPLMQFIGAPVLGRLSDRFGRKPVLIASQIGTLIGFIMLGFAN
                     SMVVLFISRIIDGLSGANISTAQAAIADSTNEKTRTQGLGLVGAAFGVGFVLGPILAF
                     IVLAASGQNYQYVAWTAALFSLISILLTTFWFKETVADAADPEASRKRRPFSFGNLRE
                     AMAHRPTIMFLVIVMFFYQIAFGGYEQLFSLFTLNRLGMDARDTSGLFMLAGILIIVI
                     QGGLIGRWSRKKGDRWLALMGIGALAIGLIGTALTPAMPVPWYDKARVLESLAGQGEF
                     RVSIQSINVSLPDEAARGWFGIIWLMIASIPAALGGGTLQPAINSLITKDSDKSEVGG
                     MLGLSAAAYSAANAIAPLFYGALFQWFGAPVPFFAGGAILLALFLFAPRVIKNS"
     gene            complement(1498..2175)
                     /locus_tag="PLT08_05735"
     CDS             complement(1498..2175)
                     /locus_tag="PLT08_05735"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HNE03996.1"
                     /translation="MPLDVYRINALCFDVDGTLSDTDDLYVQKVSKLFPRFLFKDPEH
                     AARRFVMWIEAPGNALLGLADTLHLDDEMVAVINWLSRHRKQSDKKFLLVPGVDEMLK
                     QLHGRYPMAVVSARDEKGTMRFLEQYDLVKYFDAVITGLSAVHTKPYPDPILLAAQKM
                     NVSPENCLMIGDTTVDIRAGKSAGAQTLGVLCGFGEEPELRHLGADEIVEDTTKLLEI
                     LTINRER"
     gene            2219..4348
                     /locus_tag="PLT08_05740"
     CDS             2219..4348
                     /locus_tag="PLT08_05740"
                     /inference="COORDINATES: protein motif:HMM:NF024583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HNE03997.1"
                     /translation="MTEDPNRALLELLVKVSREVATALDLRTVLQRVLYACIQYVGGE
                     RGSIVVMDDLGKPVDATIVYGKQFHDHTTQQLRETVERGLAGWVVQNRKPALVPDTSK
                     DERWLRRADDAAEKSGAKSAICVPLLARERFVGVLTLVHPVPNAFNEQHLELVQAISD
                     QASIAVLNARLYTETQRTARVMTALAEGAATINSSLEMQDVLRRILNQTMQALQVETV
                     ALGLIDSHSNDLVFQAAAGNNSGHIPGLHVPAGKGLVGKVIQAGRGVILPSIKHDPTL
                     VEEDRLNGVEMRALAIAPIQLHGKVIGVLEAVNPISGAFDPDALVVMTGLGNLAGTTI
                     DNSKLFEQLQNAHKHYRELFEDSIDPILITDWEGKVLEANRQAVELSGYTTEQLHTMS
                     IDQLHEVNWGKVGLSFETIKAGTSCTYESGMLRQDGGRLPVEVYARRVEFEDSDSIQW
                     ILRDITARKDLDALRDDMTSMIFHDLRSPLGNIVSSLEMMSTLMPEDETLSSMLNIAK
                     NSTGRIQRLVNSLLDINRLEAGQRIVDQNSIDPVALVREAIRDVEPSVTARQHVLTNR
                     ALSVLPLIWVDVDMIHRVLINLLENSIKFTPAGGRIDIGAQTNEDGLFVRFWIRDNGP
                     GIPAAERERIFEKFTRLRGKNKAGGLGVGLAFCRLAVHAHDGDIWIESEEGKGTTFWL
                     TLPVAQRKSAGQIKRQTGRLTFKPDKP"
     gene            4408..>4753
                     /locus_tag="PLT08_05745"
     CDS             4408..>4753
                     /locus_tag="PLT08_05745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE03998.1"
                     /translation="MQEITKNIFIEDQFLGVTLGVIVTPRGLIQIDAPPSPEDARSWR
                     ASLMNLGGGIDRLLINMDAHPDRTLGARAMDCTVVAHEKTAYIFRTRPSTFKAQGEET
                     GADWESIPGLGSV"
BASE COUNT         1139 a         1189 c         1234 g         1191 t
ORIGIN      
        1 ttctatcacg aactgtacat gagccagttc aagaaaaagg aagacgaaac catcgtagcg
       61 taataaaatc aacagaccct aaagtttcgc tttagggtct gtcattaaat caaatccaag
      121 gattgtccat gactgaaaca actcagactc cccccgaaga aaaacttgat ttcaaacgca
      181 tcctgccgat tctcgtcatc gtgcttgtag acttgatggg actttccatc attattccgc
      241 ttttgccttt gtacgccgcc cgcttcggtg cggaaccgat cgtggtcggc attctccaag
      301 ccacgtaccc gctgatgcag ttcatcggcg ctccagttct gggtcgcctt tcagaccgct
      361 ttggtcgtaa gccagtcctt attgccagcc agatcggcac attaatcggt tttatcatgc
      421 ttggtttcgc caacagtatg gtggtccttt tcatctcccg catcattgac ggtctttctg
      481 gagcgaatat ctctaccgct caagcagcga ttgcagacag caccaatgaa aagactcgca
      541 cgcaagggtt gggtttggtg ggtgccgcat tcggtgtagg gtttgtgttg ggtcctatct
      601 tggctttcat tgttttggct gcctcgggtc agaattatca gtacgtggca tggacagccg
      661 cgcttttctc cctcatttct attttgctaa cgacattctg gttcaaagaa acagttgcag
      721 atgccgccga ccccgaagcc tcgcgcaagc gccgtccgtt ttcgtttggt aatttacgcg
      781 aagctatggc acaccgcccc accatcatgt tcctcgtgat cgtgatgttc ttctatcaaa
      841 tcgcttttgg cggatacgaa cagctcttct cactcttcac tctcaatcgc ctcggcatgg
      901 atgcgcgtga cacctctgga ttgttcatgt tggcaggcat ccttatcatt gtgatccaag
      961 gtggtttgat cggacgttgg tctcgcaaaa agggtgaccg ttggctggca ttgatgggca
     1021 ttggtgcctt agccatcggc ttgatcggca cggctctgac tccagccatg cctgtgcctt
     1081 ggtacgataa ggcgcgtgta ctcgaaagcc ttgccgggca aggtgagttc cgtgtttcta
     1141 ttcaaagcat caacgtcagt ttgccagatg aggcggctcg cggttggttc ggtatcattt
     1201 ggttgatgat cgctagcatc cctgccgctt tgggcggtgg gacgctccag ccagccatca
     1261 atagcttaat cacaaaagat tctgataaga gcgaagtggg tggaatgctc gggttatcgg
     1321 ctgcggcata tagcgcggca aatgcgattg cccccctgtt ttatggtgca ttattccagt
     1381 ggtttggtgc gccagttcca ttctttgcgg gcggagcgat tttactggcg ctgttcttat
     1441 ttgccccaag agtgattaag aactcataac gaaagaatgt gagcgctaga cgaaggtcta
     1501 gcgctcacgg tttatagtca gaatctccaa tagctttgtt gtatcttcta caatctcatc
     1561 ggctccaaga tgtcttagct ccggctcttc gccaaaacca catagcacgc caagggtttg
     1621 tgctccagcc gatttgcccg cacgaatatc aaccgttgta tccccgatca tgaggcagtt
     1681 ttccggcgag acgttcatct tctgcgccgc aagaaggatg ggatcgggat agggtttggt
     1741 atgaaccgct gaaagccctg taatcacagc atcgaaatat ttcacaaggt catactgctc
     1801 gagaaaacgc atggtccctt tttcatctcg ggcgctgacg acagccatgg ggtaacgtcc
     1861 gtgtagctgt ttgagcatct catccacgcc ggggacgagt aggaattttt tatcagattg
     1921 tttgcggtga cgactcagcc aattgatcac agccaccatt tcatcatcca aatgaagcgt
     1981 atcggcaaga ccaagcaagg cattgccggg agcttcgatc cacatcacga agcgacgtgc
     2041 ggcatgttct gggtccttaa aaagaaaacg gggaaacaat ttcgacacct tttgaacgta
     2101 caggtcatct gtatcgctta aggtgccatc tacatcgaag catagtgcat ttatcctata
     2161 tacgtctaga ggcatggatt aattgtacca aaggggctat acttaagccg ggataagaat
     2221 gactgaagat cctaatcgtg ccctcctcga attattagta aaggtcagcc gtgaggttgc
     2281 caccgcgctg gatctccgca cggtgttgca acgggttctg tacgcttgca tccagtacgt
     2341 gggcggtgag cgtggcagca ttgtggtgat ggatgacctt ggcaaacctg tagatgccac
     2401 cattgtgtat ggaaaacaat tccatgatca caccacacaa caacttcgag aaacggtcga
     2461 acgtgggttg gcaggttggg tggttcaaaa tcggaaacca gcccttgtcc ctgatactag
     2521 caaggatgaa cgttggctgc gccgcgcaga tgatgccgcc gaaaaaagtg gagccaagtc
     2581 tgcgatctgt gtgccgcttc tggcgcgcga acgctttgta ggtgtgttga cgctggttca
     2641 ccctgttccc aatgctttta atgaacaaca tcttgaatta gtccaggcga tctccgatca
     2701 ggcgagcatt gcagtgttga atgcccgctt gtataccgag acccagcgca cggcgcgagt
     2761 tatgacggcg ctggcagaag gcgctgcaac gatcaattct tcgctggaaa tgcaggatgt
     2821 attacgtcgc atcttgaatc agaccatgca agccttgcaa gtggaaacgg ttgcgctggg
     2881 gctgatcgac tcgcatagca atgatctcgt atttcaagcc gcagctggaa ataactctgg
     2941 gcatattccg gggttgcatg taccagcagg aaaaggcttg gtagggaaag tgattcaagc
     3001 cgggcgcgga gtgattctgc ctagcatcaa acatgaccca acgttggtcg aagaagacag
     3061 actgaacgga gtcgagatgc gcgctcttgc tatcgctccg attcaattgc atggaaaagt
     3121 catcggcgtt ttggaagcgg tgaatcccat ctctggtgca tttgacccgg atgcgttggt
     3181 ggtgatgacc ggcttgggga atcttgctgg aacaaccatc gataattcga aattattcga
     3241 gcaacttcaa aatgcgcaca agcattatcg cgagttattc gaagacagca ttgacccgat
     3301 cctcattacg gattgggaag gcaaagtgtt ggaagcgaac cgtcaggcag tggagttgag
     3361 cggctatacc accgagcagc ttcatactat gagcattgac caactccacg aagtgaattg
     3421 gggcaaggtg ggtctgagct ttgaaacgat caaagctggc acctcttgta cgtatgaatc
     3481 tgggatgtta cgccaagatg gcggcagatt acccgtggaa gtatatgcac gccgagtaga
     3541 atttgaagat tctgactcca ttcagtggat cttgagagac atcaccgcgc gcaaagattt
     3601 ggatgcactg cgcgatgata tgacctccat gatcttccat gacttgcgct caccgcttgg
     3661 caatatcgtt tcaagcctcg aaatgatgag cacgctcatg ccagaagatg aaacgttaag
     3721 ctccatgctt aacatcgcca agaattctac tgggcgcatt cagcgtctgg tcaattcatt
     3781 gctagatatc aatcgtctcg aagcgggtca acgcattgtg gaccagaatt caatcgaccc
     3841 ggttgcgttg gtccgtgaag ccattcgcga tgtggaacca tcggtgacag ctcgtcaaca
     3901 tgtgcttacc aaccgcgcat tgagtgtctt gccgttgatc tgggtggacg tggatatgat
     3961 ccaccgcgtg ctgatcaact tgttggaaaa ctccatcaag ttcacgcccg ctggtgggcg
     4021 cattgacatt ggtgcacaga ccaatgaaga tggtttgttc gtcagatttt ggatacgaga
     4081 taacggtccc ggcattcccg ctgcggaacg tgaacgcatt ttcgaaaagt ttacacgcct
     4141 gcgtgggaag aacaaagcag gcggcttggg ggtagggctt gctttttgtc ggcttgcagt
     4201 ccacgctcac gacggtgata tctggatcga aagtgaagaa ggcaaaggaa ccaccttctg
     4261 gctcaccctg cccgtggcgc aacgcaaatc tgctggtcag atcaaacgcc agaccggtag
     4321 actcaccttc aagccagata agccataaaa agatagaaag aacaacgaag gacaatagat
     4381 ccgaaaggaa tttttttcag aaccatcatg caggaaatca caaagaacat tttcatcgaa
     4441 gaccaatttt taggcgtcac cttgggcgtg atcgtcaccc cgcgcggatt aattcagatc
     4501 gacgcgcctc cctctcctga agatgcccgc tcttggcgcg cctctctcat gaatttaggc
     4561 gggggcatcg accgtttact gatcaacatg gatgcccatc cagaccgcac cctcggcgcc
     4621 cgcgccatgg attgcaccgt tgtagcccat gaaaagactg cgtacatttt ccgtactcgt
     4681 cccagcactt tcaaagcaca aggcgaagaa accggcgccg attgggaatc cattcccggc
     4741 ttgggcagtg tgc
//
LOCUS       DAOQJE010000165         4748 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_10653_length_4748_cov_5.673130, whole genome shotgun sequence.
ACCESSION   DAOQJE010000165 DAOQJE010000000
VERSION     DAOQJE010000165.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 4748)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4748)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4748
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10653_length_4748_cov_5.673130"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..751)
                     /locus_tag="PLT08_05750"
     CDS             complement(<1..751)
                     /locus_tag="PLT08_05750"
                     /inference="COORDINATES: protein motif:HMM:NF014148.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE03999.1"
                     /translation="MTSTKITVIGAGSASFGENTLSAIMRSKKLSGSTLALVDRNTDS
                     LDIVRRLAERLNREWDSGFKIESYSHHRDALPDSRFVVCAIEVGARENLWQQDFEIPL
                     KYGVRQPYAENGGPGGFAHAARNIRPILDIAHDMEQACPDAWFINFTNPMVRICDAVN
                     RYSKIKVVGLCHQIYIGYCYAGLVLGDDLGFDIPKGIRNMNAEVDQHDLREQVIGQAV
                     KRLDIRAAGTNHFSWILSMVDRETGEDLYPLF"
     gene            complement(774..1895)
                     /locus_tag="PLT08_05755"
     CDS             complement(774..1895)
                     /locus_tag="PLT08_05755"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HNE04000.1"
                     /translation="MTNTNFPVQYPPHTPEPKKKISSRKIRDFFQSLFSHALINFIGL
                     FFLVPFLWMLITAFKSNQDVFHTPPRWLPYDNVLVDINGEQYPLYDVQTPDGVKQLAA
                     LKIVEGVGTFVDPASPTETIEYEMQQGNTKIAEPVMHVSFRWQNFPDAMNRASRPSID
                     ASFWVYFKNSLIIAFFSIVGTLLSNTPVAYAFARLKFPGRDFLFVIVLATMMLPYQVT
                     MIPLYLLFNNFLGWGDSFLPLIIPTFFANAYDVFLLRQFFRTIPEEMCDAARVDGASE
                     WQIFTQLVIPLSTPVLATVTVFTFLWAWNDFTGPLLFLTSPRNFTMALGLQDFQSQRT
                     LIWNQLMAASAIFTIPIIVAFFFAQKTFIQGIKLTGSKE"
     gene            complement(1900..2784)
                     /locus_tag="PLT08_05760"
     CDS             complement(1900..2784)
                     /locus_tag="PLT08_05760"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HNE04001.1"
                     /translation="MNERRSFKIGMLFLLPWTIGFLAFTLYPMLASLVYSFTIYHPRQ
                     PLEWNGIKNYTDLLTDDLFWTALYNTLYMVLIGVPLTLFSSFFCAILLNLKVRGQAYY
                     RVIYFLPSIVPTVASTILWLWILNPQAGIMNTLLSYIGIDGPNWMTNPAWSKPGLILL
                     GMWGMGNTIIIYLSGLQDVPLSLLEAAELDGASWWQRLWNITVPMVSPITLFNLITGT
                     IFMFQYFAQAYVFRGFQGLGYPLNSTLFYSVLLYQNAFQWLKLGYASAMAWVLFIIIL
                     VCTLLMMKISDKFTYYAG"
     gene            complement(2925..4226)
                     /locus_tag="PLT08_05765"
     CDS             complement(2925..4226)
                     /locus_tag="PLT08_05765"
                     /inference="COORDINATES: protein motif:HMM:NF024808.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HNE04002.1"
                     /translation="MNKKIFMLFSLFVILSMLLAACGGGKQETSQVDTLRVWIQWGDN
                     PQQIQALFDKYTAETGIKVEVTAPLETDKILPALTGSEPPDVLVLSGGDLVKSYYKEG
                     LVDDLNGAITTGGIDLNDFFDAPLNQCRSGDKILCLPWGTDMYALFWNKDLFEAAGLD
                     PETPPATMEELAEFADKLTKVAPDGSLEQIGFLPDMGWDHSDLYINMYGGFWYNEDGT
                     QMTVNSQPVLDALTWQQQFYTKYGVDNVMAFSSGWADTYMSADYPFYTGKLAMYVDGE
                     WMTGPNFINAFKPEASYGVAPFPPPADHPDRANTAVVQGTVAVIPSGAANKEASAKLL
                     AWMMSPEVVAEEFCFNANLPTSKKAAEDPCFTSNPKFKVFTDLISSPNAKIIVTTPIS
                     LEFNDAWGLVIEQVLHAGMDPKPILDDLQAEFEVKLADALK"
     gene            complement(4294..>4748)
                     /locus_tag="PLT08_05770"
     CDS             complement(4294..>4748)
                     /locus_tag="PLT08_05770"
                     /inference="COORDINATES: protein motif:HMM:NF024770.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HNE04003.1"
                     /translation="PEGHEDSLWRERGYREALEKHNLPFAPELVVMGGFEEEIAFNNI
                     QKMIQEGIQFDAVFAGDDDTAIGVYRALKASKRLIPDDVAVVGFDDVQFSRYISPALT
                     TVRAPIEEVGREAVRQLKSLMDDGQAESLVLMRTELVIRESCGCKNHP"
BASE COUNT         1382 a         1189 c         1050 g         1127 t
ORIGIN      
        1 gaaaaagcgg atacaagtcc tcccccgttt cgcggtcaac catcgacaga atccacgaaa
       61 aatgattagt tcctgccgcg cggatgtcca accgcttcac agcctgacca ataacctgtt
      121 cccgcaaatc atgctgatcc acctctgcat tcatgttgcg aatccctttc ggaatatcaa
      181 agccaaggtc atcacccaac accaaccccg cataacaata gccaatatag atctgatgac
      241 acaagcccac cacttttatc ttgctatatc ggttgacggc atcacagatc ctcaccatag
      301 gattggtgaa attgataaac catgcatctg ggcaggcttg ttccatatca tgagcaatat
      361 ccaaaatagg acggatattc cgcgccgcat gggcaaaacc accgggtcca ccattctcag
      421 catatggttg gcgcacacca tacttgagcg gaatttcaaa atcttgttgc cataaatttt
      481 ctcgcgcgcc cacctcaatg gcacaaacca caaaacggct gtcaggtaac gcatcacgat
      541 gatgtgaata ggattcgatc ttgaagccag aatcccattc tcgattaagg cgttccgcca
      601 acctgcggac aatatctagg ctatctgtat ttcggtccac aagagccaat gtagacccac
      661 ttaatttttt ggaacgcata atagcagaga gagtattctc tccgaatgaa gccgagcccg
      721 ctccaattac tgtaatcttt gtagatgtca taatcaattc cgtggttaaa aagttattcc
      781 ttcgaaccgg ttaacttgat gccttgaata aaggtctttt gtgcaaagaa aaacgcaacg
      841 atgatcggaa tcgtaaagat ggctgaagct gccatcagct gattccaaat caaggtgcgt
      901 tgactttgaa agtcctgcaa accgagcgcc atcgtaaagt tacgcgggct agtcaagaag
      961 agcagcggac ctgtgaaatc gttccaagcc cataaaaagg tgaagacggt cactgttgca
     1021 agcacaggcg tagaaagcgg aataaccagt tgagtgaaaa tctgccattc tgaagctcca
     1081 tctacacgag cggcatcgca catctcttca ggaatggtgc ggaagaattg tcgcaacagg
     1141 aagacgtcat aagcattcgc aaaaaacgta gggatgataa gcggtaaaaa gctatcgccc
     1201 caacctagaa aattattgaa caagagatat aaaggaatca tggtgacttg atacggaagc
     1261 atcatggtcg ccaagacgat cacgaacaaa aaatcacgcc ctgggaattt gagacgcgca
     1321 aaggcatatg caaccggagt attcgatagc aaagtcccca cgatagaaaa gaaagcaatg
     1381 atcaaactat ttttgaaata cacccagaaa ctggcgtcaa tgctggggcg agaagcgcga
     1441 ttcatcgcat ctggaaagtt ctgccaccga aagctgacat gcataaccgg ctcggcgatc
     1501 ttggtattcc cttgctgcat ctcatactca atcgtttcgg ttggagaagc cgggtccaca
     1561 aaagtaccca ccccttccac gatcttgagt gcggccaact gcttcacacc atccggcgtc
     1621 tgcacatcgt aaagcggata ttgctcgccg tttatatcga ccagaacatt atcgtagggt
     1681 agccagcgag gcggagtatg aaaaacatcc tgattggact taaacgccgt aatcaacatc
     1741 cataaaaatg gaacgagaaa aaacaaaccg atgaagttga tcaacgcatg gctaaacagt
     1801 gactggaaaa agtcgcgaat ctttcggctg cttattttct ttttcggctc gggcgtatgg
     1861 ggagggtatt gaacgggaaa atttgtattt gtcattggct taccctgcgt agtaagtgaa
     1921 cttatcgctg atcttcatca tcagcaacgt acaaacaaga ataataatga acaaaaccca
     1981 cgccatggca gaggcatacc cgagcttaag ccattgaaag gcattctgat acaaaagcac
     2041 actatagaag agcgttgagt tcaacggata cccaagccct tgaaaccctc gaaacacata
     2101 agcttgtgca aaatattgga acataaagat cgtgcctgta atcagattga aaagcgtaat
     2161 tggggaaacc attggaacgg taatattcca aagtctctgc caccaactgg ctccgtccaa
     2221 ctcggcagct tccaacaggc ttagcggcac gtcttgcaag cctgaaagat aaatgatgat
     2281 cgtattcccc atcccccaca tacctagcaa aataagcccc ggcttggacc atgccgggtt
     2341 ggtcatccag tttggtccgt caatgccaat atagctaagc aaggtgttca tgatccctgc
     2401 ttgcggattc aaaatccaca accacaaaat ggtgctggca acggtcggca caatagacgg
     2461 caaaaaataa atcacgcggt aataggcttg tccgcgcact tttaggttca agagaattgc
     2521 acaaaaaaag gatgagaaca acgtcaacgg aacgccgatc agcaccatat acaaggtgtt
     2581 atagagcgct gtccaaaaga ggtcatcggt cagcaagtca gtgtaatttt tgatgccatt
     2641 ccattctaaa ggctgccgtg gatggtaaat ggtaaagctg tacacaagag atgccagcat
     2701 gggataaaga gtgaaggcta aaaaaccaat tgtccacgga agcagaaaca acattccgat
     2761 cttgaaactg cgtcgttcat tcatgcccca tttcctaaat ttgggaggcc aggaaaactt
     2821 ccatcctgtt gttcgcaatt cttccatgtc ttatctccaa ttggctacga aatatcagta
     2881 tgggcggagc gcgtcttgcg ctccgcccaa aatttatgga aaggctattt cagagcgtct
     2941 gccagtttca cttcgaactc agcttgcaag tcatctaaaa tcggtttggg atccataccc
     3001 gcatgcaaaa cttgttcgat tactaatccc caagcatcgt taaattcgag cgaaataggg
     3061 gtagtgacaa tgatcttggc attcgggctt gagatcaaat cggtgaagac cttgaacttg
     3121 gggttgcttg tgaagcatgg gtcttcggct gccttcttgc tagtgggcag gttggcattg
     3181 aagcagaatt cttctgccac aacttcagga gacatcatcc atgccaacaa tttagcagag
     3241 gcttctttat tcgcagcacc gctggggatg actgccacgg ttccttgaac aacggcagtg
     3301 tttgcgcggt cggggtgatc ggctggtggc gggaagggcg caacaccata gctggcttca
     3361 ggcttgaaag cattgatgaa gttcggaccc gtcatccatt caccatctac atacatggca
     3421 agtttgccgg tatagaaggg ataatcagca gacatatatg tatctgccca accagaggag
     3481 aacgccatca cattgtcaac accatatttg gtatagaact gttgttgcca agtgagtgca
     3541 tccaacactg gttggctgtt gacagtcatc tgtgttccat cttcgttgta ccagaagcca
     3601 ccatacatgt tgatatacag gtcactatga tcccaaccca tatcaggtaa gaagccaatt
     3661 tgttcaagcg aaccatctgg cgcaactttc gtcagcttat ctgcgaactc cgccagttct
     3721 tccatcgttg caggcggagt ctcagggtca agaccggcag cttcgaacaa atccttattc
     3781 cagaacaaag catacatatc cgtcccccat ggcaaacaaa ggatcttatc gccgctgcgg
     3841 cattgattca aaggtgcatc gaagaaatca ttcaaatcaa tccctccggt tgtaatggcg
     3901 ccgttcagat catccaccag accttccttg taatacgatt tcacaaggtc gccgccgctc
     3961 aataccaaca catcaggggg ctccgagccg gtgagcgcgg gcaaaatctt atcggtttcc
     4021 aaaggcgcgg tcacttcaac cttaataccc gtttcagcag tgtatttatc aaagagggct
     4081 tgaatttgct gtgggttatc accccattga atccacacac gcaacgtatc tacctgagac
     4141 gtctcctgct tgcctccgcc acatgccgcc aacagcatgg aaaggataac gaacaggctg
     4201 aatagcatga aaatcttttt gttcatgatt ctcctccagg gaaaatgatt tggatacgac
     4261 aacaaccaaa tggatactct tcgccgcacc tccttatgga tgatttttac atccgcacga
     4321 ttcacggata accaactctg tacgcatcaa aaccagactt tccgcctgcc catcgtccat
     4381 taggcttttc aattggcgga cagcttctct accaacttct tcgatcggcg cgcgaaccgt
     4441 cgtcagcgcg ggggaaatgt atcgggaaaa ctgaacatcg tcgaaaccga caacagccac
     4501 atcgtcggga atgaggcgct tggatgcttt taaggcacgg tatacaccaa ttgcagtgtc
     4561 atcatcgcct gcaaacacag catcaaattg aatcccctct tgaatcattt tttgaatgtt
     4621 gttgaatgcg atctcttctt caaagccacc catcacaaca agctctggcg caaagggcaa
     4681 gttatgtttt tccaatgctt cgcgataacc gcgctcacgc caaagagaat cttcatgacc
     4741 ctctggtc
//
LOCUS       DAOQJE010000166         4732 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_10702_length_4732_cov_6.348728, whole genome shotgun sequence.
ACCESSION   DAOQJE010000166 DAOQJE010000000
VERSION     DAOQJE010000166.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 4732)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4732)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4732
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10702_length_4732_cov_6.348728"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1028
                     /locus_tag="PLT08_05775"
     CDS             <1..1028
                     /locus_tag="PLT08_05775"
                     /inference="COORDINATES: protein motif:HMM:NF024583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HNE04004.1"
                     /translation="KVQERTVELETRMAQLRASTLTARAIAETQDIAELLKNAANLIA
                     ERFEYYHVGIFILDDLKRNAYLQASSSEVGNSLIGQMFRLDTDRKNPLTTAIEGNRTI
                     IITDLDRSNFVRDPNFPLTRSRMIMPLSVRGKEIGVIDIHSDQPNAFTSEDAEILQTL
                     ADLTAISFDNVRLLAETRNLLTQLETNTTLQTQRTWTKFTSRQKSAYQYTPAGVRPIF
                     SQDKNNESDGLYTPIILHGQEIGRIKLKKRKGHLDEWTDRERDLVEKISSQVALALEN
                     SRLVDEAQKNALRNQMIASFSSYVRETLDVDAVIQTAATELRKVFDLKEAEIFIGMST
                     TNQTDES"
     gene            1062..3356
                     /locus_tag="PLT08_05780"
     CDS             1062..3356
                     /locus_tag="PLT08_05780"
                     /inference="COORDINATES: protein motif:HMM:NF024583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HNE04005.1"
                     /translation="MKTGSNLFAPDKNTPTKGTTSWAKLSYFQKFAIITMVFALPVLA
                     FIPLAYEQYQRIDQYGTKEAQGVLYLRNLWDLSDSLHNYVAVSTNVENGVADKTDLTR
                     AKNNVTDALNALKENTEIEKNLGLSITAQNLEDAWKNSQDINNTLQLNVTVSNVIREV
                     GDKSFLILDPDLDTYYLMDAVLLRLPENQNNLYTIERLTQKVDSGSALTPEEYQQLQN
                     DVNIIQNNLEDITKNLKVVAENNKNNVFSLALTESLDSYKSNFDTYSKEVLSYTSANS
                     SGTSSQASTLFTEVINTEAVFYNAASSALEQGIQSRVSSYSFSIEFYSIVSILSILVA
                     FTLGNQLLRSIIVPLQKTIEASEKFAAGEFHTRIDYTTADEAGLIIQAFNKLAEEIDV
                     KQNNLQLRSNALENKTSELETIAKVSREITSFRDLSSLLTAATNLIHENFGYYHVGIF
                     LLDERKEFAVLAASNSEGGKRMLERGHHLKVGETGIVGYVSQTLEARIALDVGKDAVF
                     FNNPDLPETRSELALPLVVNTQILGVLDVQSTQPEAFDQEDVKTLNILADQLAIAIQN
                     ANLFSEAEKAIEATRATYGQLSREAWNRILRSQAKVGFLATPPSTVQIQNETLESNIA
                     KAIETGDLIHDADGLTISVPVKIRGQIIGAIRLKKSDISEAWTQDETNLAIALSDQLS
                     GALESARLYRESQQRAYRESLVSDISARINASATRDLIIRETVQELGQSLVNTSVTFQ
                     LLDQSFSENPNNGNGRTAKSSRNE"
     gene            3365..>4732
                     /locus_tag="PLT08_05785"
     CDS             3365..>4732
                     /locus_tag="PLT08_05785"
                     /inference="COORDINATES: protein motif:HMM:NF024583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HNE04006.1"
                     /translation="MNNMSLRDYLFKDRPGEEFGQRAEILVAHTLAFLATLLSVLAFN
                     SPAALISSVITNSIGLVTLILAIRGNFKYLFIFPLVTSITLCIVSITDGKGSHDLIWV
                     GPMGLFLLANVYTRKNSIGPILLGLLNIALFVATGIAEIYKVIPNQYGTNTEHILLGV
                     LYLSSIMGAITGIFHRHRNLLRIAVDSKNEEEQVKKQYEEVISTLDQQVNARTAELDA
                     INQELLSKTARLQAASDISQELTKIFYEHNVDLLSYTTRLISEKLGYYHVGIFIVDKS
                     RGFAVLQAANSKGGQQMLTKHHQLKVGGTGIVGYVAQSGRPRIALDTGADAVFFNNPY
                     LPETRSEISLPIKVLNNVIGVLDVQSTQSAAFTNEDTNILMTIANQLALLLFEEEGST
                     STVSTIRKRTQFSDLERQFGYSFRTDGSVVANTTIDPSPSIKRAIASGETVTIISASK
                     DGLPML"
BASE COUNT         1463 a         1042 c          922 g         1305 t
ORIGIN      
        1 acaaagttca agaacgtaca gttgagttag aaactcgcat ggcacaacta cgcgcctcta
       61 ccctaactgc tcgtgcaatt gctgaaacgc aagatattgc cgaattgtta aaaaatgctg
      121 cgaatttaat cgctgaacgt ttcgagtatt accacgttgg aattttcatc cttgatgatc
      181 tgaaacgaaa tgcctatctt caggcttctt cttctgaagt tggaaactct ttaattggtc
      241 aaatgttccg tctcgataca gaccgtaaaa accctctcac aactgccatc gaaggaaata
      301 gaactatcat cattactgac ctcgatcggt caaattttgt tcgcgatccg aatttccctc
      361 ttacccgctc acgtatgatt atgccattgt ctgtacgtgg taaagaaatt ggagtgatcg
      421 atattcattc tgatcaaccc aatgccttta catcagaaga cgctgaaatt ttacaaactc
      481 tcgcagactt gactgcgatc tcatttgata acgtccgcct ccttgcggag actagaaact
      541 tgctcactca gcttgagact aacactacac tccagactca aagaacttgg accaagttta
      601 caagtcgtca aaagagcgct taccaataca caccagcagg tgttcgtccg attttttctc
      661 aagacaaaaa caacgagagt gatggcttat acactcccat cattttgcac ggacaagaga
      721 tcggcagaat taagctcaag aagagaaaag ggcatctcga tgaatggacg gatcgcgaac
      781 gagatctggt tgagaaaata tcttctcagg tggctcttgc tcttgaaaac agtcgtctcg
      841 tagatgaggc tcaaaagaac gctttacgaa accaaatgat tgcaagcttc tcaagctatg
      901 tccgcgaaac attggatgta gatgctgtaa ttcaaacagc tgctaccgaa ttacgaaaag
      961 tctttgacct caaggaagca gagattttta tcggaatgtc aactacgaat caaacagatg
     1021 aaagctaatt tgtagaaact tgtttcttta ctaaggtctt tatgaaaaca ggctcaaacc
     1081 tcttcgctcc agataagaac acccctacta agggcacaac ctcttgggcg aaattaagtt
     1141 atttccaaaa attcgccatt atcacgatgg tttttgcatt gccagtcctt gcatttattc
     1201 cgcttgcata tgaacaatat caacgcatag accaatatgg aacaaaagaa gctcaaggcg
     1261 ttctgtattt acgtaatctt tgggatcttt cagacagtct tcataactat gtcgcagtaa
     1321 gtaccaatgt agagaatggg gttgccgata aaactgactt aacaagggca aaaaataacg
     1381 taactgacgc gctaaatgcc cttaaagaga acacagagat tgaaaaaaat cttggattaa
     1441 gcattactgc acaaaattta gaagatgctt ggaaaaattc acaagacatc aacaacaccc
     1501 tacaattaaa tgttactgtc tcaaatgtta ttagagaagt tggcgataaa tcttttttga
     1561 ttctcgatcc ggacttggat acttactatc ttatggatgc cgttctgttg cggcttccag
     1621 aaaaccaaaa caatctatat acaattgaga gactcacaca aaaagttgac tctggaagcg
     1681 ctctaacgcc tgaagaatat caacagctgc aaaatgatgt caacattatt caaaataacc
     1741 tggaagatat tactaaaaat ctaaaagtag tggcagaaaa caataaaaac aatgttttct
     1801 cgttagcgtt gaccgagagt ttggattcat ataaatcaaa ttttgacaca tactctaaag
     1861 aggttttatc ttacacgtca gcgaatagta gtggaacatc atcacaggca agcacactat
     1921 ttaccgaagt tataaataca gaagctgttt tctataatgc tgcatcttct gctctggagc
     1981 aaggcattca aagccgtgta agctcttata gcttctcaat tgagttttac tccattgtct
     2041 ctatattaag cattttggtg gcttttacac tcggaaatca attattacgc tcaatcatcg
     2101 tacctcttca gaaaacaatt gaagcttcag agaagtttgc tgccggagaa tttcatactc
     2161 gcattgatta cactaccgca gacgaagccg gattaatcat tcaagccttc aacaagttgg
     2221 cagaagaaat tgacgtcaaa caaaataacc tccaactccg cagcaatgcc ttggaaaaca
     2281 agacaagcga gcttgaaact attgcaaaag tctcgcgcga aattacatcc ttccgcgatc
     2341 tatcatctct gcttacagct gctactaatc tcatccacga gaattttgga tattatcatg
     2401 taggtatttt tttactggat gaacgcaaag agtttgctgt tttggcagcc tctaacagtg
     2461 agggcggtaa aagaatgctc gaacgcggtc atcacctcaa agtaggcgaa actggcattg
     2521 ttggctatgt atcacagaca cttgaagccc gtattgctct tgatgtaggt aaggatgctg
     2581 ttttcttcaa caaccctgac ttacctgaga cccgttctga actagcgcta cctcttgtag
     2641 taaacactca gattctcggt gtattggatg tacaaagcac tcaacctgaa gcgtttgacc
     2701 aagaagatgt aaagacatta aatatccttg ccgatcagct tgcaattgca attcaaaacg
     2761 ccaacctttt tagtgaagca gaaaaggcga ttgaggcaac acgtgctaca tacggtcaat
     2821 tgagccgtga ggcgtggaat cgtattttgc gtagtcaagc caaggttggc ttcctcgcaa
     2881 cccccccatc tacggttcag atccaaaacg agactctgga atctaacatt gccaaagcaa
     2941 tcgaaaccgg agaccttatt catgatgctg atgggctaac aatcagcgta ccggtcaaga
     3001 tccgtggaca aatcattggc gccattcggc taaagaaatc agatatctcc gaagcatgga
     3061 cacaagacga gaccaatctt gcaattgctc tttcagatca attgagcggt gccttagaaa
     3121 gcgcccgtct ttatcgcgaa tcacaacaac gcgcctatcg cgagtcgcta gtgtcagaca
     3181 tttcagctcg tatcaatgct tctgctacaa gagatctaat catccgcgaa acagtgcaag
     3241 aattaggtca gtcgctagta aatacttcgg tcacattcca attgttagat cagtctttta
     3301 gcgagaatcc aaacaatgga aatggaagaa ccgcaaagag ttccaggaat gagtaacgtc
     3361 acttatgaat aacatgagtt tacgcgatta tctattcaaa gatcgtccag gtgaagaatt
     3421 tgggcaacga gccgagattc ttgttgcgca tacactggca ttcttagcta ctcttttatc
     3481 ggtactggca tttaactcac cagctgcttt aatttcttct gtgatcacga attcaattgg
     3541 gcttgttact ctcattctag ctattcgcgg gaatttcaag tacctattta tatttccact
     3601 tgtaacttct attactcttt gcattgtatc catcacagac gggaaaggtt ctcacgatct
     3661 gatttgggtt ggtccaatgg ggctattctt gctagccaat gtttacacgc gtaaaaattc
     3721 aataggtcct attcttcttg gcttattaaa tattgcctta tttgtagcaa ctggtatagc
     3781 agaaatatat aaagtcatac ctaaccaata cggcactaat acagaacata ttcttctggg
     3841 cgtcttgtac ttatcctcta tcatgggtgc aatcacaggt attttccacc gtcatcgtaa
     3901 tctccttaga attgctgttg atagtaaaaa tgaagaggaa caggtcaaga aacaatatga
     3961 agaagtcatt tccactcttg atcaacaagt gaatgcacgt actgctgagc tagatgcgat
     4021 taaccaggaa ttactaagca agaccgcacg cttgcaggct gcatcagata tttcacaaga
     4081 gttgaccaaa atcttctacg aacacaatgt agatttgctt tcctacacta cacgattgat
     4141 tagtgagaag cttggctatt atcatgttgg gatttttatt gttgataagt ctcgcgggtt
     4201 tgctgttctt caagcagcca acagcaaagg tgggcaacaa atgctcacaa aacatcatca
     4261 actcaaagta ggcggaaccg gcattgtagg ttatgtcgcg cagagtgggc gtccacgcat
     4321 cgctcttgat acaggggcag atgcggtctt cttcaacaac ccttacctgc cagaaacacg
     4381 ttctgaaata tcactaccta tcaaggtttt gaacaatgtt attggtgtat tagacgtgca
     4441 aagcacacag tctgcggcat ttacgaatga agacacaaac atccttatga ccattgctaa
     4501 tcaattagca ctacttctat ttgaagaaga aggctcaaca tctacagtct ctactatcag
     4561 aaagcgtact caattctcag accttgagag acagttcgga tattccttcc gcaccgatgg
     4621 aagtgtggtc gcaaacacaa caattgaccc cagcccgtct attaaacgag ctattgcctc
     4681 aggcgagact gttaccatta tcagcgcttc aaaagatggg ttgcccatgt tg
//
LOCUS       DAOQJE010000167         4719 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_10737_length_4719_cov_5.920240, whole genome shotgun sequence.
ACCESSION   DAOQJE010000167 DAOQJE010000000
VERSION     DAOQJE010000167.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 4719)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4719)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4719
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10737_length_4719_cov_5.920240"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..624)
                     /locus_tag="PLT08_05790"
     CDS             complement(<1..624)
                     /locus_tag="PLT08_05790"
                     /inference="COORDINATES: protein motif:HMM:NF014042.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-phospho-L-lactate transferase CofD family
                     protein"
                     /protein_id="HNE04007.1"
                     /translation="MKIVAFAGGVGGAKLAQGLAQTLPPKDLTIIVNTGDDFEHLGLY
                     ICPDLDTVCYTLGGLANPETGWGRAGETWNTIANVEKLGGPAWFRLGDSDIATHLERT
                     RRLKEGKSLSEITKEFCKAWGIEATVLPMTDSPVRTMVDSDEGELAFQEYFVHRQCQP
                     RVKGFRFDGVEVAEPAQGVREALASADAIIFCPSNPWVSIDPILSVVK"
     gene            complement(626..1252)
                     /locus_tag="PLT08_05795"
     CDS             complement(626..1252)
                     /locus_tag="PLT08_05795"
                     /inference="COORDINATES: protein motif:HMM:NF013077.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HNE04008.1"
                     /translation="MTNIIDLHTFLRTRRSIRRFKPDPIPDSVIREILHTATLAPSAH
                     NRQPWRFVVMTDKTSIAEFAKTMAIEFRHVLEQDQLPPEEIQKRLNRSQERISGAPVV
                     IIACVEMTEMEKYPDERRKKAEYLIATQSVANAGMQLLLAAHAEGLGAVWICSPLFAQ
                     SAVQEALRLQPTWEPQAMFLVGKPVEVPAVRERKPIEEISIFIDKKGN"
     gene            complement(1236..1628)
                     /locus_tag="PLT08_05800"
     CDS             complement(1236..1628)
                     /locus_tag="PLT08_05800"
                     /inference="COORDINATES: protein motif:HMM:NF013412.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HNE04009.1"
                     /translation="MPFPENFLDLIQPETKSFLYLATVNAKGVPQVTPVWFDTDGEHI
                     LINTNEGRLKDRNMKKNENVAMVIQDPKSPYRYLGMQGKVVSYTTEGADEHINLLSLR
                     YDEEPWKYREGQKRIIFKIKPTSFDEHN"
     gene            complement(1702..2472)
                     /gene="cofE"
                     /locus_tag="PLT08_05805"
     CDS             complement(1702..2472)
                     /gene="cofE"
                     /locus_tag="PLT08_05805"
                     /EC_number="6.3.2.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043773 - coenzyme F420-0 gamma-glutamyl
                     ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="coenzyme F420-0:L-glutamate ligase"
                     /protein_id="HNE04010.1"
                     /translation="MSLTLNTLKNIPLIRQGNNLADILLKSLHESGIALQDNDILVLA
                     QKIVSKTEGRMVNLAKIEPSSAALELAPIIDKDARLIELILQESNEVLRIRKGVIVVE
                     HKLGFVCANAGIDHSNVMGDGKEDEEYVLLLPENPDTSARWLREEILRKTDKTVGIMI
                     IDSHGRAWRNGTVGVCIGLSGIPALVDERGWKDLFGYTLKATIVGVADELAAAASLMM
                     GQAAEGTPVVHVRGFPYPLSESGSLQELIRPKEMDMFR"
     gene            complement(2527..3201)
                     /gene="npdG"
                     /locus_tag="PLT08_05810"
     CDS             complement(2527..3201)
                     /gene="npdG"
                     /locus_tag="PLT08_05810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent F420 reductase"
                     /protein_id="HNE04011.1"
                     /translation="MKEDHILVSVAVLGGTGKEGKGLAYRWARAGYHVLIGSRSEEKA
                     VAAAKEVTEMLGDGVSVAGMNNLQAAKEANIVVLTVPYAAHRETLESVKDALQGKILV
                     DVTVPLVPPKVATVQMPAAGSAAQEAKQILGEGVEVCAAFQNISHEHLLGGEGVECDV
                     LVTGTSKDARANVILLVEAAGLRGWDAGPIENSVVVEGLTSVLIGINKKYGSTHAGIK
                     ITGISK"
     gene            complement(3225..4577)
                     /gene="ssnA"
                     /locus_tag="PLT08_05815"
     CDS             complement(3225..4577)
                     /gene="ssnA"
                     /locus_tag="PLT08_05815"
                     /inference="COORDINATES: protein motif:HMM:NF005540.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative aminohydrolase SsnA"
                     /protein_id="HNE04012.1"
                     /translation="MLITNANIITWESPNRVLSNYAILIEGGKIKEIGTTKSLTAKHP
                     KVKATDVNGQYVMPGGICAHTHFYGAFARGMAIPGPAPKDFPEILKKLWWPLDRSLDA
                     EGVRYSALVSIVDAIKHGTTTLIDHHASPNTIDGSLDVIADVVDKSGLRGVLCYEVTD
                     RDGIEKANAGIQENVRFIKRLASNPHPRLAAAFGLHASLTLSGATLQACAAAAPEGTG
                     FHIHTAEHESDEYDSLQKSGMRVIDRLLKHGILGPKTITAHGVHFDAREMEILKETET
                     WLTHQPRSNMNNGVGTAQIESMLRLGIKVCLGNDGFTNSMWDDWKVAYLLHKAEHRDP
                     RRMGGYEVQQMAIYNNAALAQNFFPEAPVGQIIPGAAADLIFVDYHPFTPLTDGNLPW
                     HIIFGFQQSMVTSTMVAGKFLMKDRELLTLNEEEIAARAREIAPRIWKKYEEEVAKVM
                     "
BASE COUNT         1099 a         1168 c         1152 g         1300 t
ORIGIN      
        1 tttgacaacg ctcagaatag gatcaatact gacccacgga ttggatgggc agaaaatgat
       61 cgcatctgca gacgcaagcg cctcacgcac gccctgagct ggttcagcga cttcgactcc
      121 gtcgaacctg aagcctttca ctctcggttg gcattgtctg tggacgaaat attcttgaaa
      181 tgccaattcg ccttcatcag agtcgaccat ggttcgcaca ggcgaatccg tcatgggcaa
      241 cactgtcgct tcaatgcccc aagctttgca gaattctttt gtgatctctg aaagagactt
      301 cccttctttt agtcgtcgcg tgcgctcaag atgggttgca atatccgagt cgccaaggcg
      361 aaaccatgcc ggtccgccaa gcttttcgac gttagctatg gtgttccacg tctcgccagc
      421 ccgaccccaa ccggtctcgg ggttggcaag cccgcccaag gtgtaacaaa cggtatcaag
      481 gtctgggcag atataaagcc ccaaatgttc aaagtcatca ccagtgttga cgataatggt
      541 taagtctttt ggtggaaggg tttgagcgag tccttgtgcc agttttgctc cgccaacccc
      601 gcctgcaaat gctacgattt tcaatttagt ttcccttttt atcaatgaag atcgaaattt
      661 cttcgatcgg tttgcgttct cgcaccgccg gaacctctac tggtttaccg accaggaaca
      721 tagcttgagg ttcccatgtg ggttgaagtc ttaatgcttc ttgcacagca gattgagcaa
      781 ataatgggct gcatatccat acggcgccca agccttctgc atgcgctgcc agtaaaagct
      841 gcatgccggc attggcaact gattgcgttg cgatcagata ttcggctttc ttacggcgtt
      901 catctgggta tttttccatt tcggtcattt caacacacgc gataatgacg actggtgcgc
      961 cgctgattct ttcttgggag cgatttaggc gtttttgaat ctcttcgggc ggaagttgat
     1021 cttgctcaag cacatgcctg aactctattg ccatcgtctt tgcaaactca gcgatagacg
     1081 ttttatctgt catcaccaca aaacgccaag gctgacggtt atgcgcggat ggggcgagag
     1141 tggcagtgtg gagaatttcc cggatgactg aatcaggaat cgggtccggt ttgaagcggc
     1201 gaatcgagcg ccttgtccgc agaaaggtgt gcagatcaat tatgttcgtc aaaggaagtc
     1261 ggcttgatct taaaaataat ccgcttttgc ccctcgcggt atttccatgg ttcttcatcg
     1321 taacgcaaag aaagcaagtt gatatgctca tctgcgcctt cagttgtgta agaaaccact
     1381 ttgccttgca tgccgagata acgatacggg cttttcgggt cttgaatgac catcgccacg
     1441 ttttcgttct ttttcatatt gcgatctttg aggcgacctt cgtttgtgtt aataagaata
     1501 tgttcaccat ctgtatcgaa ccataccggg gtcacttgtg gaaccccctt tgcattgacg
     1561 gtggcaagat aaagaaaaga tttggtctca ggttgaatga ggtcgaggaa gttctcaggg
     1621 aagggcattg tattctccag gaattttatt cgagccttgc gtacctttta tatcaagggc
     1681 gcataagatt cctgttagag cttatcggaa catatccatt tctttggggc gaataagctc
     1741 ttgaagtgag ccgctttcac tgagcgggta cggaaacccg cgcacatgta caaccggcgt
     1801 accttctgcg gcttgaccca tcattagtga agcggcggcg gcaagttcat ctgccacacc
     1861 aacaatagtt gctttgagcg tgtacccaaa gaggtctttc catccgcgtt catcgacaag
     1921 ggctggaatt ccgctgagcc cgatacaaac acctaccgtt ccattgcgcc aggcacgccc
     1981 atgcgagtcg atgatcataa tgccgacggt tttatctgtt ttgcgtagta tttcttcgcg
     2041 caaccatctt gcagatgtat ctgggttttc tggcagaagc agtacatatt cttcatcttc
     2101 tttgccatcg cccatgacat tggaatggtc aatgccggca ttggcacata caaacccaag
     2161 tttatgttca actacgatta cccctttgcg aatacgtaaa acttcattgc tctcctgcaa
     2221 gatcaattcg atcaggcgag catctttatc aatgattggg gcaagttcaa gtgccgcaga
     2281 tgaaggttcg atctttgcaa ggttgaccat gcgtccttcg gttttgctaa cgatcttctg
     2341 tgcaagcacg agaatgtcat tatcttgcag agcaatgcct gattcgtgca atgatttaag
     2401 aagaatatcc gccaggttgt taccttggcg gatgagagga atatttttga gtgtgtttag
     2461 agtgagagac atgatacgcg ttttgtaatg cgcaacacgt caaatgtacg cattacgcac
     2521 ggaggcttat ttagagattc cggtgatctt tattccggca tgggtagaac cgtacttttt
     2581 attgataccg atcaaaacgc tggtgagacc ttcaactaca actgaatttt caatcggtcc
     2641 tgcgtcccag ccacgtagac cagccgcttc cactaacagg atgacatttg cacgcgcatc
     2701 cttactggta cccgtaacaa gtacatcgca ttccacgcct tcgccgccga gcaagtgctc
     2761 atgcgaaata ttctggaacg ctgcacaaac ttccacgcct tcgccgagaa tttgtttggc
     2821 ttcctgtgcc gcagaacccg ctgccggcat ttgcactgta gccactttcg gcgggacgag
     2881 aggtaccgtc acatcaacaa ggattttgcc ttgcaatgca tctttgacgg attccagtgt
     2941 ttcgcgatgt gccgcatatg gaacggtcaa cacgacgatg ttcgcttctt tcgccgcctg
     3001 aagattgttc attccagcga cagagactcc atcgccgagc atttctgtca cttctttggc
     3061 tgccgcaacc gctttttcct ctgaacgaga accgatcaat acatgatacc cggcgcgagc
     3121 ccaacggtag gctagccctt tgccttcttt gcctgtgccg cccaagacag caactgaaac
     3181 taaaatgtga tcttctttca tgagttctcc taaatggacg ttctctacat gaccttggcg
     3241 acttcttctt cgtacttttt ccaaatgcgc ggagcgattt cccttgcgcg cgctgcaatt
     3301 tcttcttcgt tgagagtgag caactcacga tctttcatca aaaattttcc agctaccata
     3361 gtgctagtca ccatactttg ttgaaaacca aagatgatgt gccatggcag gttgccatct
     3421 gttagcgggg tgaaggggtg ataatccaca aagatcaagt ctgcggcagc accggggatg
     3481 atttgcccaa caggtgcttc ggggaagaag ttctgagcaa gtgcagcatt gttgtagatc
     3541 gccatctgtt gcacttcata cccgcccatg cggcgtggat cgcgatgttc ggctttatgc
     3601 aggagatatg ccactttcca atcgtcccac atggaattgg tgaagccatc attgcccaaa
     3661 caaaccttta tgccaagccg gagcatggat tcgatttgag ctgtgcccac accattattc
     3721 atattagaac ggggctgatg agtaagccag gtttcggttt ctttgaggat ttccatttca
     3781 cgggcatcaa agtggacacc atgtgccgtg atggtctttg gacctaagat gccatgtttt
     3841 agcaagcggt caatcacacg catgccagat ttttgcaagc tatcgtattc atcggactca
     3901 tgctcggctg tatggatgtg gaagcctgtc ccttcggggg ctgcggcggc acaggcttgc
     3961 aaggtcgcgc ccgaaagtgt caggctggca tgcagcccaa aagcagctgc caaccgcggg
     4021 tgtggatttg aggcgagacg tttgatgaag cgcacattct cttgaatccc tgcattggct
     4081 ttttcgattc cgtcacggtc tgttacttcg tagcaaagca caccacgtaa gccgctttta
     4141 tctaccacat cagcaatgac gtctagcgag ccgtcaatag tgttgggaga tgcatggtga
     4201 tcaatgagag tagtggtgcc atgcttgatt gcatccacga tagaaaccaa ggcagagtat
     4261 cgcacgccct ctgcatcaag agaacggtca agtggccacc ataatttttt gaggatttca
     4321 ggaaaatcct ttggcgccgg acccggaatt gccatgccgc gcgcaaaagc accataaaag
     4381 tgagtgtggg cgcaaatgcc gcccggcatc acgtactgac cgtttacatc ggtggctttt
     4441 actttgggat gtttggctgt aagcgatttg gttgtgccga tttccttgat ctttccgcct
     4501 tcaataagaa tggcgtaatt tgaaaggacg cgattgggag attcccatgt gatgatgttt
     4561 gcgtttgtga tcagcatatt agttcaatcc atattcgcta gggtgcggta atttgacgcc
     4621 cgctgcgcca agttcccaaa ggatgttgaa ggttgcttcc cgtaattctg ggtcatcttt
     4681 gtacatggct tcatacaatt gtttgagcac aatctcttg
//
LOCUS       DAOQJE010000168         4713 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_10751_length_4713_cov_5.892658, whole genome shotgun sequence.
ACCESSION   DAOQJE010000168 DAOQJE010000000
VERSION     DAOQJE010000168.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 4713)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4713)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4713
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10751_length_4713_cov_5.892658"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..530
                     /locus_tag="PLT08_05820"
     CDS             <1..530
                     /locus_tag="PLT08_05820"
                     /inference="COORDINATES: protein motif:HMM:NF014038.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="class IV adenylate cyclase"
                     /protein_id="HNE04013.1"
                     /translation="IQPRVHEVNLRFDNEAGELRKNFKVLRLRQDIEAKFTFKGPSEE
                     RAGGVLSRKEIEFSVGSFEAAKEFLELLGFVPVVFYEKYRTTYEFLGVHIMLDELPYG
                     TFVEIEGEDIEALQKVANLLGLNWNAMVRAGYHALFERITEKYNLEYGQLSFEALKSI
                     IATAEDFGITYSDIN"
     gene            536..1030
                     /locus_tag="PLT08_05825"
     CDS             536..1030
                     /locus_tag="PLT08_05825"
                     /inference="COORDINATES: protein motif:HMM:NF023629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3601 domain-containing protein"
                     /protein_id="HNE04014.1"
                     /translation="MNRSTPNPLFTALLLFVSFVFFAMVIWIMDNPFPTSWNIIAVLM
                     GLFFITFLWGFLRILRARDIKNSQLIQSRPSAPPPLPTPETEGTVYGLYPGWEYIVVQ
                     SFTDYYGNIFEVNQKLKFKGRHFLPYQGGHTIMFEECSLYLQEDQNKNILDNFSSYII
                     KIKQ"
     gene            1060..1830
                     /locus_tag="PLT08_05830"
     CDS             1060..1830
                     /locus_tag="PLT08_05830"
                     /inference="COORDINATES: protein motif:HMM:NF016107.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HNE04015.1"
                     /translation="MQIKGLDTLARHVPELNSSSGRMKIGVTFFAIFGLTTFYFIITD
                     QIPTWTLDSQIVVTALGYLILSRVFRQKKAFIEKHKEQAFAVAFKQFTVPSLAIFSAL
                     IIHIAYMNGPKFTQPTLTTILTWLGWVCILIGTPLFIRALTILGADHATMLYVYFPGG
                     ELTHSSIYNIVRHPMYAGMLRICIGLACLNRGIFALTFVIILPILVFGWIRLIEEKEL
                     IERIPNYIEYRKQVPAFFPYPKQIFRFFKFVLTGQSYA"
     gene            1823..2314
                     /locus_tag="PLT08_05835"
     CDS             1823..2314
                     /locus_tag="PLT08_05835"
                     /inference="COORDINATES: protein motif:HMM:NF016001.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family deazaflavin-dependent
                     oxidoreductase"
                     /protein_id="HNE04016.1"
                     /translation="MLEKRPNSLLKFFFKTPLFMHKLGLGGWERLIGAQWMFLTTIGR
                     KTGKCRETLVDVMDYDSATDTYFIEAAYGTRADWYKNIQAHPVFEAQVGRRKFNASAK
                     ELNSQDTGETLVQFFRKKPAYTRSVMAMVGLKFKDENELRALGDKLTLLAVTPLSHDR
                     SAK"
     gene            complement(2280..3098)
                     /gene="murI"
                     /locus_tag="PLT08_05840"
     CDS             complement(2280..3098)
                     /gene="murI"
                     /locus_tag="PLT08_05840"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008881 - glutamate racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HNE04017.1"
                     /translation="MNLNPIGVFDSGVGGLSVLREIRNLMPNEDILYFGDQGHVPYGP
                     RPMEQIQDFSEGITRFLLKERSKIIVVACNTASAAALKYLRQAFPNVQFVGMEPAVKP
                     AAEKTQTGKVGVLATPATFQGALYASVVERFANGVELFQNTCNGLVQQIEKGNLDGEE
                     TRKILEGALHPMLEKKIDTVVLGCTHYPFVIPLIEKIVGENVRVIDPAPAVAKQVKRL
                     LEAGEALNQKEQLGSTRFMTSGDVEVVKSSLKILLQLEREVEAVTWLNDREIAA"
     gene            complement(3117..3827)
                     /locus_tag="PLT08_05845"
     CDS             complement(3117..3827)
                     /locus_tag="PLT08_05845"
                     /inference="COORDINATES: protein motif:HMM:NF019342.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HNE04018.1"
                     /translation="MTKPLIGITTFNRNNDYGKPMSGVQHTYIRAVVQGGGIPILIPA
                     ILDEEDRNDLYARVQGVLFTGGGDVSIKYFNGVDHPKIAEVDESRDITEISLLKKTVE
                     DGKPFLAVCRGVQVMNVALGGTLYTHIPDQFPNALHHDNEEFTEIIHPVNINEDSRMA
                     EIFGETLLNVNSLHHQGLKDVASPLKVVGHAPDGMIEAVELPDHPYAMGVQWHPEWLT
                     DQPVMRRLFKSFVDASLQ"
     gene            complement(3938..4285)
                     /gene="rplS"
                     /locus_tag="PLT08_05850"
     CDS             complement(3938..4285)
                     /gene="rplS"
                     /locus_tag="PLT08_05850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HNE04019.1"
                     /translation="MADLIKAVEAKDNEKIPQLAAGDTVSVHVKIKEGDRERIQEFKG
                     LVIRLRNSGNNASVTVRRMASNGVGVERTFLLRSPRVDKIVVERHGKVRRAQLYYMRE
                     RTGKSARIKEKFS"
     gene            complement(4371..>4713)
                     /locus_tag="PLT08_05855"
     CDS             complement(4371..>4713)
                     /locus_tag="PLT08_05855"
                     /inference="COORDINATES: protein motif:HMM:NF014819.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="beta-ACP synthase"
                     /protein_id="HNE04020.1"
                     /translation="INAHGTGTSLNDQAETRAVKAAFGDLAYKMPISSTKSMTGHMMG
                     ATGALEVIFCVNAIRDGILPPTIHYETPDPECDLDYIPNKAREAKISLAISNAFGFGG
                     HNAVLAIRKYS"
BASE COUNT         1270 a         1152 c         1000 g         1291 t
ORIGIN      
        1 taattcagcc gcgtgtccat gaagtcaatt tacgattcga taatgaagcc ggggaattaa
       61 gaaaaaactt caaggtactt cgcctgcgac aggatatcga agccaaattc acgttcaaag
      121 gtccaagtga agaacgcgcg ggcggcgtac tcagccggaa agagattgaa ttctctgttg
      181 gcagttttga agcagccaaa gaatttctag aattacttgg tttcgtgcct gttgtctttt
      241 atgaaaagta tcgcacaacc tatgaatttc ttggcgtgca cattatgtta gatgaattgc
      301 cttatggtac ttttgttgaa atcgaaggcg aagatattga agcacttcaa aaggtagcga
      361 atcttcttgg gttgaactgg aacgctatgg tcagggcggg gtatcacgca ctgtttgagc
      421 gcattacaga aaagtacaac cttgaatatg gtcagttgag ttttgaggca ttaaagtcca
      481 tcattgccac ggctgaagat ttcggaatca cctattctga tataaactag aatttatgaa
      541 ccgttctacg cctaatccgc tcttcactgc tctattgctt tttgtcagtt ttgttttctt
      601 tgccatggtg atatggatca tggataatcc attccctact tcatggaaca tcatcgccgt
      661 tctcatgggg ctgtttttca tcacgttttt atggggattc ttgcgtatct tacgagcaag
      721 agacataaaa aattctcaac ttattcaatc cagaccgtcc gctccgccac ctttaccaac
      781 tccagaaaca gagggaactg tctatggact ttacccaggc tgggaataca tagtagtaca
      841 atcttttaca gactactatg gcaatatctt tgaggtgaat caaaagctca aattcaaagg
      901 acgccatttt ttaccgtatc aaggcgggca tacgattatg tttgaagaat gtagccttta
      961 tttacaagag gaccaaaaca agaatattct cgataatttt tcgagttata ttatcaaaat
     1021 caaacaataa actaacaggt ttcaaacaaa gagattgcca tgcaaatcaa aggtttagat
     1081 actttagcaa ggcatgttcc agaattaaat tcatcatcag ggcggatgaa aatcggcgta
     1141 acattcttcg ccatatttgg tttaaccacc ttttatttca tcatcaccga ccaaatcccc
     1201 acatggactc ttgatagcca aattgtcgtc actgcattgg gttatcttat tcttagtcga
     1261 gtcttcaggc aaaagaaagc ttttattgaa aagcacaaag agcaagcctt tgccgtggca
     1321 ttcaaacaat ttacagtgcc aagtcttgct attttttctg cattaattat tcacatcgct
     1381 tacatgaatg gaccaaaatt tacccagcct acacttacaa caatcctcac ttggcttggc
     1441 tgggtttgta ttcttattgg aacgccccta tttattcgtg cgcttaccat attgggtgca
     1501 gatcatgcca ctatgctata tgtgtatttt ccaggcggcg agctgacaca ctcaagtatc
     1561 tacaacattg ttcgtcaccc catgtatgca ggcatgcttc gcatctgcat tgggttagcg
     1621 tgcctcaacc gcggaatttt tgcgctgacc tttgtaatta ttctacccat ccttgttttt
     1681 ggttggatcc gtctcataga agaaaaggaa ctaatagaac gcattcccaa ctatattgaa
     1741 tatcgaaaac aggtgccggc attctttccc tacccgaaac aaatattccg ctttttcaaa
     1801 tttgttctaa caggtcaatc gtatgcttga aaaacgcccc aactctctac tgaagttttt
     1861 cttcaaaact cctctcttta tgcacaaact cggtttaggt ggttgggagc gcctcattgg
     1921 ggcgcaatgg atgtttctca ccaccatcgg cagaaaaacc ggcaaatgcc gcgaaacgct
     1981 tgtagatgtg atggactatg actctgccac agacacctac ttcattgaag ccgcctatgg
     2041 cacccgtgca gattggtata agaacattca agcgcaccca gtttttgaag cacaggtcgg
     2101 taggcgaaaa tttaacgcca gtgctaaaga gttaaactca caagacactg gcgaaacgtt
     2161 ggttcagttt tttagaaaaa agcctgctta tacccgctcc gtgatggcaa tggtcgggtt
     2221 gaaattcaaa gatgagaatg agcttcgcgc attaggcgac aagctaactc tcttagcggt
     2281 cacgccgcta tctcacgatc gttcagccaa gtaaccgctt cgacttcccg ctcaagctgc
     2341 aacaaaattt tcaaactgga tttgaccacc tcgacgtcac ccgatgtcat aaagcgtgtg
     2401 ctcccaagtt gctccttttg gttcaaagct tcgcccgcct caagcagacg tttgacctgt
     2461 tttgccactg ccggagctgg gtctatcacc cgcacgtttt cgcccactat tttttcgatc
     2521 aacggaatca caaaagggta atgagtgcag cccaacacaa cagtatcgat cttcttttca
     2581 agcatggggt gaagagcacc ttctaagatt ttgcgagttt cttcaccatc caaattcccc
     2641 ttctcgatct gttgcacaag tccgttgcag gtattttgaa ataactccac gccatttgcg
     2701 aaacgctcca cgacagaagc ataaagcgct ccctgaaacg tagccggtgt tgccagaacg
     2761 cctactttcc ccgtctgtgt tttctctgct gctggcttaa ccgccggttc catgcctacg
     2821 aattgaacgt taggaaaagc ctggcgcaaa tatttcaatg ccgcagccga agccgtatta
     2881 cacgccacca caatgatctt ggacctttcc ttcagcaaaa agcgcgtaat gccctcagaa
     2941 aaatcctgta tctgctccat cgggcgcgga ccgtatggga cgtgcccctg atcgccaaag
     3001 tatagaatgt cttcatttgg catcaagttt ctgatctctc gcaacacaga aagtccacct
     3061 acaccggaat caaacacacc tatagggttt aaattcatat cgccaaaacc cggcatttat
     3121 tgaagagacg catccacaaa cgatttgaac agtcggcgca tcacaggctg atcggtaagc
     3181 cattcggggt gccattgaac gcccatagcg tacggatggt ctggcaattc tacagcttcg
     3241 atcatgccgt ctggagcatg accaactact tttaagggtg aggcgacgtc cttcaatccc
     3301 tgatggtgca agctattgac gttcaacaaa gtttccccaa aaatctctgc catacgagaa
     3361 tcctcgttaa tgttaactgg gtgaataatc tcagtaaatt cttcgttatc gtgatgcaac
     3421 gcattaggga attgatctgg gatatgtgtg tacagagtgc cgcctaaagc gacattcatt
     3481 acttgaacac ctcggcacac cgcgagaaaa ggctttccat cctccactgt ttttttcagc
     3541 aatgaaatct cagtgatatc gcgcgactca tcgacctctg caattttcgg gtggtctacg
     3601 ccgttgaaat atttaataga cacatctccg ccgcctgtaa ataaaacacc ttgcacccga
     3661 gcatacaaat cgtttcggtc ttcttcatcc aaaatcgccg ggatcaagat cggtatgccc
     3721 ccaccttgaa ccaccgcacg aatataggtg tgttgcacgc cagacatagg cttgccgtaa
     3781 tcattattgc gattgaaagt tgtgatgccg attaatggtt tggtcacagg ttcctccaaa
     3841 acaagaaatt tacgaatata agcataccac agccaaacaa gcggttataa acaaaaaaat
     3901 cccgatgggg ttgccatcgg gattcaataa ggaaaaacta ggagaacttt tccttgatgc
     3961 gggcgctctt gccggtgcgt tcgcgcatgt agtagagctg ggcacggcgg accttaccgt
     4021 ggcgttccac cacgattttg tccacgcgcg gggagcggag caggaaggtg cgttcgacgc
     4081 caacgccatt cgacgccatg cggcgcacag tcaccgaagc gttgttgccg ctgtttcgca
     4141 ggcgaattac caagcctttg aattcctgaa tacgctcacg atcaccttcc ttaattttca
     4201 cgtggacgct cacagtgtcg ccagcagcaa gctgggggat cttctcgtta tcttttgcct
     4261 ctacggcttt aatcaagtct gccatttcgt tcttccttac tttatgatta ggtttccaat
     4321 atacgcgagg gcggattata gcacggataa tctcaaatca caagcaaaaa tcaggaatat
     4381 tttcgaattg ccagcactgc gttgtgcccg ccaaaaccga aagcattgct gattgccagc
     4441 gaaatcttcg cctcacgagc cttgttcgga atatagtcca agtcacattc ggggtcgggg
     4501 gtttcgtagt ggatggttgg cggcaaaatc ccgtctcgaa tggcgttcac acagaaaatc
     4561 acttccaaag caccggttgc acccatcata tgccctgtca tagacttggt cgaggaaatc
     4621 ggcattttgt acgccaagtc accaaatgcc gccttaacag cccgggtttc ggcttggtcg
     4681 ttcaaagaag tgccagtacc atgtgcattg ata
//
LOCUS       DAOQJE010000169         4706 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_10784_length_4706_cov_7.226618, whole genome shotgun sequence.
ACCESSION   DAOQJE010000169 DAOQJE010000000
VERSION     DAOQJE010000169.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 4706)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4706)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4706
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10784_length_4706_cov_7.226618"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            161..1288
                     /locus_tag="PLT08_05860"
     CDS             161..1288
                     /locus_tag="PLT08_05860"
                     /inference="COORDINATES: protein motif:HMM:TIGR00200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CinA family nicotinamide mononucleotide
                     deamidase-related protein"
                     /protein_id="HNE04021.1"
                     /translation="MTSAEIITIGTEILLGEIVDTNTRYIARTLRSLGVDLYRTVTIG
                     DNTDRIAEAIQRSMERANIIITTGGLGPTVDDPTREAVAQAVGVQTEFREDLWQQVVE
                     TIGRYGRTPSENQKRQAYVPQGALGLRNPVGTAPCFIVETETNAVISLPGVPAEMEHV
                     LHESVIPYLQKRFGLNEVIKVRVLHCAGLGEGMIDEKIADLEELSNPTVGLAAHTGVV
                     DVRITAKAKSESEANEMIAKIEADVRQRLGNVVFGADEDKLEEVTLDMLAKRGLTLTA
                     IESGLDGLLARKVPHTASKPDLDPAALMDALRAARADSKADIALGVSVYVNERAAEMA
                     MITAKGEKSHRITYGGPPKSLPRWSMNLALNWLRTTLEEMK"
     gene            1288..2286
                     /locus_tag="PLT08_05865"
     CDS             1288..2286
                     /locus_tag="PLT08_05865"
                     /inference="COORDINATES: protein motif:HMM:NF012471.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M14 family metallopeptidase"
                     /protein_id="HNE04022.1"
                     /translation="MAKPKPRKKNDTPLVILAWGLNIFGLCALALVGVAYFALNPVSA
                     ATQAVSVNPAYTATMPPTYTPDVLSINLPTITPNPLSTMIVVQSPTPFVLQNAGTLGR
                     ASVATVGYSVSGRPIEVYMFGNGEHEKMIVAGIHGGYEWNTIALADELIQYIFDHPEV
                     IPSDVTLYVLRNMNPDGDARDHGIDGRVNDHGVDLNRNFPANWAKTWDRTGCWDLTKT
                     TGGTGPGSEPETKAVMYFLQSHNVETLISYHSAALGVFPGGIPWEADSKQFAKALAKA
                     TNYPYPPIDTGCVYTGTLADYAVELGVTAVDMELSTHKYTDFDQNVKALEVLLNWR"
     gene            2339..4312
                     /locus_tag="PLT08_05870"
     CDS             2339..4312
                     /locus_tag="PLT08_05870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HNE04023.1"
                     /translation="MTQTIDTLFINAIVLTMDEKLTQYDPGAVAVNGDSIVAVGAEAE
                     LKKEYSAKEVVDCGGKILMPGLVNAHTHVPMTLVRGLADDLRLDVWLMGYMLPVERQF
                     VSPEFVRLGTLIACAEQIRSGVTTFNDMYFYEDDIAQATADAGMRAVCGESVMKYPAP
                     DAASYDDSLAYAREFIKKWKGHPLIVPAIAPHAPYSTNPEIIRACVDLAKEFDVPLHI
                     HISETAFEVENMRKEQGMPVVPYVKKLGLFEAKVIAAHCVHIDTGEIRTLQHYKAGVS
                     HNPSSNLKLASGFAPVQKMLEDGLNVGIGTDGPASNNDLDMFEEVRLASFVAKAASND
                     PTVLPAATALTMATRLGAQALHIGHLTGSLEVGKRADLILVDTNPLHNTPRFKRDPQN
                     AYAQLVYASKSTDVTDVMVNGKWLMRDHQLLTLNEKELIAQARELATKIDAFLTEREQ
                     SVLSKLIALGGSMEEESFEVQVKVKVEEPNLIVQNVKRPEIEINAYKHYRQFDEYFLF
                     EDPNQGRLRYREDSLINEKGDIVNVRSRLTLLGVKREDEMGEVLLSRSRFLAPAAQSL
                     RFYREYFKPKSEISVEKNRFRWHIKYKNTEFFINLDEVKEPAMGHFLEVKSRTWSRKD
                     ADLKADLVRELLDILGVGNAETVTQDYIEILTA"
     gene            4383..>4706
                     /locus_tag="PLT08_05875"
     CDS             4383..>4706
                     /locus_tag="PLT08_05875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE04024.1"
                     /translation="MEPFRKPIATFFATSFVVTAVMAILFLNFDRRAFSAETYQQAFA
                     REDFYNKIPNLMAQSIVSGANMGQLPTVTQGMSLETWENFIRILLPPEVLKPIGDDVL
                     ISTFAY"
BASE COUNT         1219 a         1239 c         1141 g         1107 t
ORIGIN      
        1 gcaaattgat gagtgagttt attgtggatg gaaaattctc aagcgtggat gtttcaatgc
       61 ttgacctgaa acgcttcgaa gaaggtcgtt tgataaacga gtacaatgta gtttgacctc
      121 acccctgccc ctctcctaaa agagaggggg gaaattgaat atgacctcag cagaaatcat
      181 tactatcggt acagaaatat tactcggcga aatcgtagat acgaacactc gctacatcgc
      241 gcgcacgctt cgcagcctgg gcgtggattt atatcgcacc gttaccattg gcgataacac
      301 agaccgtatt gccgaagcca ttcaacgttc gatggagcgc gctaacatta tcatcaccac
      361 tggcgggctg ggtcccaccg tggatgaccc cacacgtgaa gccgttgcac aagccgttgg
      421 cgtgcaaact gaatttcgtg aagacctgtg gcagcaagtg gtcgagacta ttggtcgtta
      481 tgggcgcaca ccttctgaga atcaaaaacg tcaggcatat gttccacaag gtgcgcttgg
      541 attgcgcaac ccggttggaa cggcaccttg ttttattgtt gaaacagaaa ccaatgccgt
      601 catttctttg cccggcgtgc ccgccgaaat ggaacatgtc ctgcatgagt cggtcattcc
      661 gtatttgcaa aaacggttcg ggttgaatga agtgatcaaa gtccgcgtgt tgcattgcgc
      721 gggtttaggc gaaggcatga tcgatgaaaa gatcgcagac ctagaagaat tgagcaaccc
      781 aaccgttggg ctggcggcac ataccggcgt ggtagatgtt cgcatcacgg caaaagccaa
      841 aagcgagtct gaggcgaatg aaatgatcgc taaaattgaa gcggatgtgc gtcaacgact
      901 tggcaatgta gtcttcggcg ctgacgaaga taagctcgaa gaagtgacat tggatatgct
      961 cgccaaacgc ggcttgaccc tgaccgccat cgaaagcgga ctcgacggat tgctcgcccg
     1021 caaagtgccg cacaccgcct ccaaaccgga ccttgaccct gccgctctta tggacgcttt
     1081 acgcgccgct cgagcagact caaaagcaga cattgcgctt ggcgtttcag tttacgtcaa
     1141 tgaacgtgct gctgagatgg caatgatcac cgccaaaggg gaaaaatccc accgcatcac
     1201 ctacggcggt ccgcccaaga gcctgccgcg ttggagcatg aatttagcat tgaattggct
     1261 gcgcacaacc cttgaggaaa tgaaataatg gcaaagccca agccacgcaa aaagaatgac
     1321 acaccgttgg tcatccttgc atggggcttg aacatctttg ggttatgtgc gcttgcattg
     1381 gttggagtgg catactttgc gttgaacccg gtttcggctg ccacacaggc tgtttctgtg
     1441 aacccagcct ataccgcaac catgccgcca acctacacgc cagatgttct ctcgattaat
     1501 ttaccgacca ttaccccaaa ccctctttca acgatgatcg tggtgcaatc tcccactccc
     1561 ttcgtgctgc aaaacgcagg gacgttaggg cgtgccagcg tcgccacggt gggatattcc
     1621 gtttctggca gacccattga agtgtatatg tttggcaatg gtgaacacga aaaaatgatc
     1681 gtagccggga ttcacggcgg ttacgaatgg aataccattg cattggctga tgagttgatc
     1741 caatatatct tcgatcatcc agaagtgatt ccaagcgatg tgaccctgta tgttctgcgc
     1801 aacatgaacc cggatggtga cgctcgcgac cacggtattg acgggcgtgt caacgaccac
     1861 ggtgtggact tgaaccgtaa ctttcccgcc aattgggcaa aaacctggga tcgcaccggc
     1921 tgttgggacc tcaccaaaac caccggggga acgggtcccg gctcggagcc ggaaaccaaa
     1981 gcagtgatgt acttcctgca atcacacaat gttgagacgc tcatcagtta tcacagcgcg
     2041 gcactgggtg tcttccccgg cggcattcca tgggaagctg attcaaaaca gtttgccaaa
     2101 gcgcttgcaa aagcgaccaa ctatccctac ccccccatcg acacgggttg tgtttacaca
     2161 ggcaccttgg cagattacgc agtagagctt ggtgttacag cagtagatat ggaactttct
     2221 actcacaagt acaccgactt cgatcaaaat gtaaaagctt tagaagtgct cttgaattgg
     2281 agatagcttc tatttcatat cgctcagggc gataatcaaa ctataaaaag gagattgaat
     2341 gacccaaact atagataccc tctttatcaa tgccatcgtg ttgacgatgg acgaaaaact
     2401 cactcaatat gaccccggcg cagtagctgt aaacggcgat tccatcgttg cggttggtgc
     2461 tgaggctgaa ctaaaaaaag aatattctgc gaaagaagtt gttgactgcg gtggcaagat
     2521 tctcatgccc ggtctggtca acgcgcatac ccatgtaccc atgaccctcg tccgcgggct
     2581 tgcagacgac ctacgcctcg acgtctggct aatgggttat atgctgcccg ttgaacgtca
     2641 attcgtttcg ccagagttcg tgcgccttgg aaccctcatt gcctgcgccg aacaaatccg
     2701 tagcggtgtt actaccttca acgatatgta cttttatgaa gatgatatcg cccaagccac
     2761 tgccgatgca ggcatgcgcg cagtatgtgg cgaatcggtt atgaaatacc ccgcgcctga
     2821 tgccgcttct tacgatgact cgcttgcgta tgcacgtgaa ttcatcaaaa aatggaaagg
     2881 gcatccgctt attgttccgg ctattgcgcc acatgccccc tactccacca accctgaaat
     2941 tatccgcgcc tgtgtagacc ttgccaaaga gttcgatgtg ccgttgcaca ttcacatttc
     3001 tgaaaccgcc ttcgaagtgg aaaacatgcg caaagaacaa ggcatgccgg tggttccgta
     3061 tgtcaaaaag cttggcttat tcgaagccaa agtcattgcc gcccactgtg tccacattga
     3121 tactggtgag atccgcactc tgcaacacta caaagccggt gtttctcata acccatcttc
     3181 aaatctcaaa cttgcctccg gctttgctcc cgttcaaaaa atgctcgaag atggactcaa
     3241 cgtaggcatc ggtacggatg gtcccgcttc aaacaatgac ctcgacatgt tcgaagaagt
     3301 gcgcctcgcc tctttcgtcg ccaaggctgc ttcaaacgac ccaaccgttc tccccgccgc
     3361 caccgcgttg accatggcaa cccgcctggg ggctcaggca ttgcacatcg gtcaccttac
     3421 cggctcgttg gaagttggca aacgagcaga cctgattctc gtggatacca atccactgca
     3481 caacacaccg cgcttcaagc gtgacccaca gaatgcctat gcccagttgg tatacgcttc
     3541 taaatcgaca gacgttaccg atgtgatggt caacggcaag tggctgatgc gcgatcatca
     3601 attgcttacg ttgaacgaaa aagaacttat tgctcaagct cgcgaactcg caacaaaaat
     3661 cgacgccttc ttgaccgaac gcgaacagtc tgtgctttcg aagctcattg ccctcggcgg
     3721 ttcgatggaa gaagaatcat tcgaggtgca ggttaaggtc aaggtcgaag agcccaatct
     3781 catcgtccag aatgtgaagc gccctgagat cgaaatcaac gcctacaagc attatcgtca
     3841 attcgatgaa tacttcctgt tcgaagatcc caatcaaggg cgtttacgtt accgcgaaga
     3901 cagtcttatc aatgaaaaag gtgacatcgt caatgtgcgt tcacgtttga ctctattggg
     3961 tgtgaagcgc gaagatgaaa tgggagaggt gttgctctcg cgcagccgct tccttgcgcc
     4021 tgccgcacag tcgctgcgtt tttatcgtga atacttcaaa cccaaatctg agatttccgt
     4081 cgaaaagaat cgcttccgct ggcatatcaa gtataagaac accgagttct ttatcaacct
     4141 cgatgaagtg aaagaacccg ccatgggaca cttccttgaa gtgaaaagcc gcacctggag
     4201 ccgaaaagac gcagacctca aagcagacct cgtgcgagaa ctgcttgata ttcttggcgt
     4261 tggcaatgct gaaaccgtca cccaagatta cattgaaatt ttgaccgcgt aatcaatacc
     4321 aagggcgacc tgcctgccat ggtgtttatg gcatcgggtc gcccttatga atttaattaa
     4381 ctatggaacc ctttagaaaa ccaatagcaa cattctttgc cacatcgttc gttgtcacag
     4441 ccgtgatggc gatcttattc ttgaactttg accgccgcgc tttcagcgcc gaaacctatc
     4501 aacaagcgtt tgcacgcgaa gacttttaca acaaaatccc caacctcatg gcgcaatcca
     4561 ttgtatcggg tgcaaacatg gggcaattgc caaccgtcac tcaaggcatg agccttgaga
     4621 cttgggagaa tttcattcgc attctgctgc cgcccgaagt cttaaaaccg attggtgatg
     4681 atgtgttgat ttccaccttt gcctat
//
LOCUS       DAOQJE010000170         4698 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_10823_length_4698_cov_6.488262, whole genome shotgun sequence.
ACCESSION   DAOQJE010000170 DAOQJE010000000
VERSION     DAOQJE010000170.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 4698)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4698)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4698
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10823_length_4698_cov_6.488262"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1217
                     /locus_tag="PLT08_05880"
     CDS             <1..1217
                     /locus_tag="PLT08_05880"
                     /inference="COORDINATES: protein motif:HMM:NF027651.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="cytochrome c-type biogenesis CcmF C-terminal
                     domain-containing protein"
                     /protein_id="HNE04025.1"
                     /translation="KRGLLKHWNMILIILTYGLVIFGTFLTRSGVLSSVHAFANSPIG
                     PFFMGFVSLTLIASIALLIKRWPELKSETEMKSMLSREALFLLNNLLFMGILVVCFWG
                     VISPLASELFTGTKITVGPPYYERATGPLFAGLLFLMAVAPLSAWGHSTVQTLGRAIW
                     KSAALATLVTVALIFTYTKNIYALIGFFAIALVLFATTHEFFRGAVARMNAQKENFFV
                     AVGRLMGRNRRRYGGYIIHISMALMAIGILGIEIFQQETQGTIAAGESLELGEYRVEY
                     RELASWDEFNAGVNYTRSVVDIYENGVFIGKLYPRIDYYYDSQQNMTIPGQHATLRDD
                     LYILLVDWQPVSSEGATFKIYVNPLVNWLWIGSLTFLFGLIFAAWPDRDPADEPLRRK
                     VSAGNRQTSAAD"
     gene            1267..1758
                     /locus_tag="PLT08_05885"
     CDS             1267..1758
                     /locus_tag="PLT08_05885"
                     /inference="COORDINATES: protein motif:HMM:NF015853.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type biogenesis protein CcmH"
                     /protein_id="HNE04026.1"
                     /translation="MKKFLFILTLMLVASGLFTAVAFAQDKTPTDDEVNAIAKQLYCP
                     VCENTPLDVCPTEACHQWRELIRQMLAEGKSEQQIKDYFVANYGARVLSEPPRAGFNW
                     LVYIIPPVLILVGAVFLFNAFRAWTKPKSAEAGSGQAEEAGTSSSDDEYIKRLEEELK
                     NRK"
     gene            1791..2360
                     /locus_tag="PLT08_05890"
     CDS             1791..2360
                     /locus_tag="PLT08_05890"
                     /inference="COORDINATES: protein motif:HMM:NF020123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA family protein disulfide reductase"
                     /protein_id="HNE04027.1"
                     /translation="MTETTAPARKSVPLWVQVTIWVFLAGLLALVGFGLNRASHPMAE
                     VGDTVPDFKLVYYEGYEHNNTPEMKLSDLRGKVVLINIWASWCKPCEQEAPELEQAWQ
                     LYKDQGDVVFIGVDYVDTPEGAFGYLKKFGITFPNAPDLQSNISSILNRQMGVPETYL
                     IDRDGVLRQIKIGPFASVSEIQAFIASAE"
     gene            2370..2879
                     /locus_tag="PLT08_05895"
     CDS             2370..2879
                     /locus_tag="PLT08_05895"
                     /inference="COORDINATES: protein motif:HMM:NF024637.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HNE04028.1"
                     /translation="MELGSIFLILAVLVAVGMYLYAPFMYRSRKLSTDEMHEISALKA
                     ERDRVINSLQELDFDFKLGKIPAEDYPEQRAELLKKGSDILRKLDELEPILSSARDAA
                     SRIEKAAAAKRADSSSTASDMSDEDIEAMISARRKQHKSKSAGFCPSCGKPVLTADNF
                     CPSCGKSLK"
     gene            2899..>4698
                     /locus_tag="PLT08_05900"
     CDS             2899..>4698
                     /locus_tag="PLT08_05900"
                     /inference="COORDINATES: protein motif:HMM:NF012264.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HNE04029.1"
                     /translation="MKLRHLFLLAIGTLLLSACNMTLAADVTPPPGYVSPTPPPTLGP
                     LYPDKAPNVENGKAIFTEKCAACHGEKGLGDGEQSKDLPVSVIPIGLPEFANEATLSS
                     WYAVVTQGRIERFMPPFNSLTDQERWDVVAYALTLHTTNEQLELGKKLFEENCADCGD
                     AFTNLQMMSALSEDDIVKFVREGNGNFPAFGSNFSDEEAYAVAAYIHTLTFAVPSAPV
                     AAAATETPAAAESTPSTDSTPADGTPQAEGTPAATEAAVASMVKGFIINNSSADLSAD
                     QQITLRGFDHGSDPSAGPTEVLNVQGTVNPDGSYSFDVELLEGQIYLAELDVDGITYQ
                     SEFAVVPAGATELALPDITVYDTTTELTDLVIDSIAVHFDYTDPESISAFVVYLLRNT
                     GDKNIKVQLENNNQDIPFIKAPANAKLLGFQQTQDGATLLATKEQDGFYIQPSDQTYG
                     LIAFSSLPRTNKVDFSQEFLLPVGSGKVFLPNGVTAQGDQLTDQGMVPLQDSSGKNYE
                     YQTYSFTNVSANSTLDFTLKGDPSETSATADLTQNQYVLIGVGAFGITLILAGVFMYL
                     RDRKKAAEESEEAEDDEEDDDQFDDTESIMDAII"
BASE COUNT         1163 a         1267 c         1105 g         1163 t
ORIGIN      
        1 aaaagcgcgg tttgctcaaa cactggaaca tgatcctcat catcttgacc tatggactcg
       61 tgatcttcgg caccttcctg actcgctcag gtgtactttc ttccgtccat gcctttgcga
      121 acagcccgat tggtccattc ttcatgggct tcgtcagcct aaccttgatt gcttcaattg
      181 ctttactgat caaacgctgg cccgaattga aaagtgaaac tgagatgaag tccatgcttt
      241 cacgtgaggc gctcttcctt ctcaataact tgcttttcat gggcatctta gtagtttgtt
      301 tctggggtgt gatctcgcca ttggcgtctg agcttttcac cggcaccaaa attacggttg
      361 gacctcccta ctacgaacgt gctacaggtc cgctatttgc tgggttgctc ttcttgatgg
      421 cagttgcgcc actctctgct tggggacatt ccaccgtgca gactctcggt cgcgccattt
      481 ggaagtctgc tgctctcgcc accctcgtga ccgtggctct gattttcacc tacacaaaga
      541 atatctacgc cttgatcggg ttcttcgcga ttgcgttggt attgttcgcc accacccatg
      601 aattcttccg tggcgcagtt gccagaatga acgctcagaa agagaacttc ttcgttgccg
      661 taggtcgctt gatgggacgc aatcgccgcc gttatggtgg ctacatcatt cacatcagca
      721 tggcgttgat ggcgatcggt attctcggca ttgaaatctt ccaacaagaa actcaaggca
      781 caattgcagc cggtgagtcg cttgaattgg gcgaatatcg cgtggaatat cgtgaactcg
      841 cttcatggga tgagttcaat gcgggtgtga actacactcg ttcggttgtg gatatttacg
      901 aaaacggcgt cttcattggc aaactctatc cgcgcattga ctattactac gactcacagc
      961 agaacatgac catccccggt cagcatgcca ctttgcgtga tgacttatac atcctgctcg
     1021 tggattggca gccggtttcg agcgagggtg ccaccttcaa gatatacgtc aacccgttgg
     1081 tgaactggtt gtggattggc agcctgacct tcttattcgg tctcatcttt gccgcctggc
     1141 ccgaccgtga cccggctgat gagcctctac gccgtaaggt gagtgcgggc aatcgccaaa
     1201 cgagtgcagc cgactaaaag agtcatcggt cgaaggtcaa ctttcgatcg atctcggaga
     1261 acttcaatga agaaattcct ctttattctt acccttatgc tggttgcctc tggtctcttc
     1321 acggcggtgg catttgccca agacaaaacc cctactgatg atgaagtgaa tgcaattgcc
     1381 aagcaattgt attgcccggt ctgtgaaaac acgccgttgg atgtatgccc aaccgaagct
     1441 tgtcatcaat ggcgcgagtt gatccgccag atgttggcag aaggcaagag tgagcaacag
     1501 atcaaagatt attttgtagc aaactacggc gctcgtgtgc tctctgagcc gccacgggct
     1561 gggttcaact ggttggtgta tatcattcca ccagtattga tccttgtggg agccgtcttc
     1621 ctcttcaatg cgtttcgagc gtggaccaag cccaagtccg ctgaggcagg ttcaggtcaa
     1681 gcagaagagg cgggcacgtc atccagtgac gatgaataca ttaagcgtct tgaagaagaa
     1741 ttgaagaaca gaaaataaca gggaccaatc ggtcttttac ggagaatgca atgacagaaa
     1801 caacagcgcc agccagaaag agcgtcccct tgtgggtgca agtcaccatt tgggttttcc
     1861 ttgcgggttt gctcgcattg gtgggtttcg gtttgaaccg cgccagccac cccatggcag
     1921 aagtgggcga taccgtgccc gatttcaagt tggtttatta cgaaggctac gaacacaata
     1981 atacgcctga gatgaagctt tctgatttgc gcggcaaggt tgtgctcatc aacatttggg
     2041 cttcatggtg caagccctgc gaacaggaag ctcctgaact tgaacaagct tggcagttat
     2101 ataaagacca aggtgatgtc gttttcatcg gcgtggatta tgtggatacg cccgaaggtg
     2161 cgtttggcta tttgaaaaag tttggtatta cattccccaa tgcgccagat cttcaatcaa
     2221 acatctccag cattttgaat cgtcagatgg gcgtacctga aacctatttg atcgaccgcg
     2281 acggtgtctt gcgccagatc aagatcggtc ccttcgcatc ggtttctgaa attcaagctt
     2341 ttattgcaag tgcagagtag ggggttctca tggaactagg ttcaatcttt ctcatcctcg
     2401 cggttctcgt cgcagtagga atgtatctat atgcaccttt catgtatcgt tcgcgcaaac
     2461 tttcaacgga cgaaatgcat gagatctcag ccttgaaagc cgaacgcgac cgcgtcatca
     2521 actcgttgca agaattggat tttgatttca agctcggcaa aattcctgct gaagattacc
     2581 ccgaacaacg cgctgagtta ttaaagaaag gctctgatat cttacgcaaa ctcgatgaac
     2641 tcgagccgat cctttcttct gctcgcgatg ccgcctcccg cattgaaaaa gcggctgccg
     2701 ccaaacgcgc agactcttcc tccaccgctt ctgatatgag cgatgaagat atagaagcaa
     2761 tgatctctgc ccgccgcaaa caacacaaaa gcaaatctgc cgggttctgc ccttcgtgcg
     2821 gaaaacccgt gcttactgcc gataacttct gcccatcttg cggaaagtct ttgaaataat
     2881 ccgcaaaccg aggaattcat gaaattacgt cacctctttc ttttagccat aggcaccctc
     2941 ttgctctctg cctgtaatat gactctcgcg gcagatgtta ctccgccgcc tgggtatgta
     3001 tcccccacgc ctccgcccac attgggtccg ctatatccag ataaagctcc caatgtcgaa
     3061 aatggcaaag ccatcttcac tgaaaagtgc gcagcatgcc acggagaaaa aggcttgggc
     3121 gatggtgaac agagcaaaga cctgcctgtc tccgtcattc cgatcggttt gcctgaattt
     3181 gcaaatgaag ccacactttc aagttggtat gccgtagtaa cccagggacg cattgaacgc
     3241 ttcatgccgc ccttcaacag cctcaccgac caggaacgtt gggatgtggt ggcgtatgct
     3301 ctcacacttc acaccacaaa cgaacagctt gaactcggca agaaactctt cgaagaaaat
     3361 tgtgccgatt gtggtgatgc attcaccaac ttacaaatga tgtcggcgct atcagaagat
     3421 gacatcgtca agttcgtgcg tgaaggcaat ggaaacttcc cagcctttgg aagcaatttc
     3481 agcgacgaag aagcgtacgc cgttgcagcc tacatccata cgttgacgtt tgcggttcct
     3541 tccgcgccgg tagctgctgc agccaccgaa actcccgccg ccgctgaaag cactccatcc
     3601 accgatagca cacctgctga tggcacaccc caagctgaag gtacaccagc agcgacagaa
     3661 gctgcagttg ccagcatggt taagggtttc atcatcaata attcaagcgc agacctttct
     3721 gcagaccagc agatcacctt gcgcggcttc gaccacggca gtgacccaag cgcaggtccg
     3781 accgaagtac tcaacgtgca aggcaccgtc aaccccgatg gttcatattc cttcgacgta
     3841 gaactgcttg aagggcaaat ctatctcgca gagttggatg tagacggcat tacctatcaa
     3901 tctgaattcg ctgttgtgcc cgccggcgct accgagctcg ctctgcccga catcaccgtc
     3961 tacgacacca ccaccgaatt gaccgacctt gtgatcgaca gcattgcagt ccactttgat
     4021 tacaccgacc ccgaaagcat tagcgcgttt gtggtctatc tcttgcgcaa cactggcgat
     4081 aagaacatca aagtacagct cgaaaacaac aatcaagata tcccgttcat caaagcacct
     4141 gccaatgcca aactgcttgg cttccaacag acacaagatg gcgcaacctt gctcgccacc
     4201 aaagaacaag atggcttcta cattcagccc agcgaccaaa cctatggctt gatcgctttc
     4261 tccagcctgc cacgcacaaa caaagttgat ttctctcaag agttcttgct tcccgttggc
     4321 agtggaaaag tattcttgcc caatggcgtc actgcacaag gagaccaact cacagatcaa
     4381 ggcatggttc cgcttcaaga ctcaagcggc aagaactatg aatatcaaac ctattcattc
     4441 acaaacgtga gcgccaactc tacattagac ttcacgctca aaggcgatcc ttccgaaacc
     4501 tctgccactg cagacctcac ccaaaatcag tacgtattga tcggtgtggg tgcctttggt
     4561 attacgctca tccttgcagg tgtcttcatg tacttgcgcg accgcaagaa agccgccgaa
     4621 gaatcagaag aagccgaaga cgatgaagaa gatgacgacc aattcgatga caccgaatcc
     4681 attatggatg ccatcatc
//
LOCUS       DAOQJE010000171         4698 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_10824_length_4698_cov_4.935387, whole genome shotgun sequence.
ACCESSION   DAOQJE010000171 DAOQJE010000000
VERSION     DAOQJE010000171.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 4698)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4698)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4698
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10824_length_4698_cov_4.935387"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(117..1004)
                     /locus_tag="PLT08_05905"
     CDS             complement(117..1004)
                     /locus_tag="PLT08_05905"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HNE04030.1"
                     /translation="MPKSKVIPYLEALFAVLVWGGTFIATKIALSEVSPATIVWIRFG
                     MGTVILGIVVVARKQFALPEKSEWLYFAMLGFLGVTFHQILQATGLQTAKATTTAWIV
                     ATTPVFIAILGWVALKEKLNGLQILGIVLAGFGVLLIVSKGNIGALFTGEEGTFGDFL
                     VLISSVNWAVYTILSRRELARHPAARMMFFVMLIGWLLVNVWIFGFGPGVSEIPQLTS
                     SGWVAILILGIFGSGLAYIAFYDALQEIPASQLGAFFNVEPLVTTILASFMINEAITA
                     ITLIGGAIIILGIWLVNRK"
     gene            1235..1882
                     /locus_tag="PLT08_05910"
     CDS             1235..1882
                     /locus_tag="PLT08_05910"
                     /inference="COORDINATES: protein motif:HMM:NF024874.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HNE04031.1"
                     /translation="MEITWYGHSCFRLTERNYATVVTDPFDHKAVGYSALKLKADIVT
                     VSHDAPGHANLEAVKGSEHVIDGAGEFEIGGVFITAVQTAGGGKKSKDKTRNTLYVFD
                     YEGITVAHLGDLQEVPTQSEVEALGTVNILLIPVGGGSSLNAAKAAEVVSVIEPNLVI
                     PMHYSTDAAKIKLDPLNKFLKEMGLGKVESLPSLKATRSSLPQETKVVVLDYQQG"
     gene            1884..2186
                     /locus_tag="PLT08_05915"
     CDS             1884..2186
                     /locus_tag="PLT08_05915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE04032.1"
                     /translation="MAVKVIMTWDIAAERDQEYFEFIIGEFVPGVQRLGLQPAEAWAT
                     LYGSYPQIQVGLLATDAPEARRVLASAEWAMLQDRLFGYVKNYSYKVVPVRTGFQF"
     gene            2249..4657
                     /locus_tag="PLT08_05920"
     CDS             2249..4657
                     /locus_tag="PLT08_05920"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HNE04033.1"
                     /translation="MPNKFSKILPWFFFAAAFQSLLAVAALLRIPSEGLSLARLALLG
                     VIGGIFLLSIGLGLARRNLLRFEWLSTTPVIISSALLSLTFSLLLFLLRYFNPERFLP
                     YYERMSPLLWLLFFLAAEAALCFLFLKNGFHPQSLTNYSSAFRASVIPLLVLFTIFLF
                     VAITKIGVTPDTGYWGEPGVAIQGWHFVIALLLGCTVLILSSSNSSLFIRYLPFVLYA
                     IAAILWLSVPLETLANSFYVSTTPPTNMPLPYSDAGFYDFSAQSLLIGTGYFGGIPPR
                     PLYVVFLSILHLVFGQNYPAVINAQVLVLAFFPVALYFLAKKLHSPAAGVTVALFAIF
                     REYTGLWIASNTRVANSKVFTTDFPTAFAIVIICLVILWWLERRDLRSTLIAGGAFGI
                     FLLFRSQSMLTIPFLFLLVLLVFNFKWSEWIKSGIIFAVTMLLAVIPWLTHNYTIAGK
                     FSFDDPNQVAVIYSQYAFTGNLDLSQFNPETDSVRERIISFTLENPAYVANFITSHFL
                     NTEIGGLLALPLIKPFNGLQEPVNLYWIEWDGNLEWYNVILVLFYLAMVALGVGSAWR
                     RLKWMALVPLAFNLGYAVSNGIARFSSWRYNMPIDWVFYFYFAIGIVEVFSILAQLFG
                     AQVKEAQTETPIFKPIQLSDFRVQYLLPVFAFVFVGSLPWLAKELVQPRYMSTQDELV
                     AKLVTAGYDQNEIDIFLQQPKAILNEGRLLFPRMYWRGEGLASANPWPAYAVQDFPRI
                     GFILINTNSQNLIYPTKEVLDFPQGADSIVLACAGDNDLLYVRVIAFDDATYQNTPLT
                     EPCP"
BASE COUNT         1130 a         1137 c         1090 g         1341 t
ORIGIN      
        1 cgatctgtgc aaaacgatag gcagacgaat ctccggctgg gattttcatc aaatagcctt
       61 tttcggtctt tgtgatgcta gcttcagggg tagtgcgtgc ttttaatggc atttgattat
      121 ttccgattta ccaaccaaat acctaatatg ataattgcgc cgccgatgag agtgattgcc
      181 gtgatggctt cgttgatcat gaatgaagcc agaatggtag tgaccaacgg ttcaacattg
      241 aagaatgccc caagttgtga ggcggggatt tcttgcaagg catcatagaa tgcaatgtac
      301 gccaaccctg aaccgaagat accaagaatc agaatggcaa cccagcccga agacgtgagc
      361 tgcggaattt cactcacacc cggaccaaaa ccaaaaatcc acacattgac caaaagccag
      421 ccgatcagca tgacgaagaa catcatccgt gccgcagggt gacgtgccaa ctcacggcgc
      481 gagaggatgg tgtacaccgc ccagttgacc gagctgatga ggacgaggaa atcaccgaag
      541 gtcccttctt cgccagtgaa aagcgcaccg atattgccct tactgacgat gagcaaaacc
      601 ccaaacccgg caagaacaat accgagaatt tgcaacccgt tcagtttttc tttgagtgct
      661 acccaaccga gaatggcaat gaaaacgggc gtggttgcca cgatccatgc agtggtggtg
      721 gctttggcgg tttgtagccc cgtagcttgc aggatttgat gaaaggtgac acccagaaaa
      781 ccgagcatgg caaaatatag ccactcgctt ttttcaggca gggcaaattg cttgcgggcg
      841 acaacgacaa taccgaggat gactgttccc atcccaaaac ggatccacac gatggtagca
      901 ggggagactt cgctgagcgc gatcttggtg gcgataaacg tcccacccca aacgaggacg
      961 gcaaataaag cttcgagata gggaatgact ttagattttg gcataaggtt tcctgaggaa
     1021 agagttggag aattataatt gttgtttact acactacttt aaggggtatt tttcctaagc
     1081 ggaattcacc atttgggtag ggattttgaa ggtgttgtga tggtcgggaa atagtcccac
     1141 caaaaactaa gacctatttg actattagac gaccaaactt cattttgtcc gtgtacccat
     1201 cgggtataat atctccctcg gaggcactag ctacatggaa attacatggt atggacattc
     1261 ttgttttaga ctgacagaac gcaattacgc gactgttgtc accgacccgt tcgatcacaa
     1321 ggcggtgggc tacagtgcgc tcaaactaaa agcggatatt gtcaccgtca gccacgatgc
     1381 acccggtcac gccaatttag aggcagtgaa gggtagtgag catgtcatcg acggcgcggg
     1441 cgaatttgag atcggcggtg tatttattac agcggttcaa actgccggag gcggcaagaa
     1501 aagtaaggat aagacccgca acaccctcta tgtgttcgat tacgaaggta tcactgtggc
     1561 acatctcggc gacttgcagg aagtgcccac tcaaagcgaa gtagaagcct tgggaacagt
     1621 gaacattctc ctcatcccgg tgggaggcgg aagcagtctc aacgccgcca aagctgcgga
     1681 agtggtcagc gttatcgagc cgaatcttgt catccccatg cattactcca ccgacgccgc
     1741 caagatcaag ctcgacccat tgaacaaatt tctcaaagaa atgggtctcg gcaaggttga
     1801 gtctctgcct tctttgaaag ccacccgttc ttcattaccg caagaaacca aggtggttgt
     1861 gctcgattac caacaaggat agtatggcag ttaaagtcat catgacctgg gatatcgccg
     1921 ctgaacgcga ccaggaatat tttgaattca tcatcggtga attcgtaccc ggtgtgcaac
     1981 ggcttggctt acagcccgcc gaagcttggg ctacccttta cggttcatac ccgcaaattc
     2041 aagtaggctt gcttgccact gatgcacccg aagcgcgccg tgtgttggca tctgctgagt
     2101 gggcgatgtt gcaagaccgt ttgttcggct atgttaaaaa ctattcttat aaagttgtac
     2161 ctgtaagaac tgggtttcaa ttttagatac caatacacca tcattgcgag gagggtgata
     2221 gctctcccgc aatgacggaa taggtcacat gccaaataaa ttttcaaaaa ttctgccgtg
     2281 gtttttcttt gccgcggcat ttcagtctct actcgccgtc gctgcactgt tgcgaattcc
     2341 atctgaaggc ttgtcgctgg cgcgccttgc cttgcttgga gtaataggag gcatcttttt
     2401 attaagcata ggattgggac ttgcacgccg caacctttta cgcttcgaat ggctttcgac
     2461 aacaccggtt attatctcgt ccgcgcttct ctcgctgact tttagcctgc ttctgttcct
     2521 tttacgttat tttaaccctg agcgttttct accctactac gagcggatga gtccgctgct
     2581 ttggctttta ttcttccttg ccgctgaagc cgctctctgc ttccttttcc tcaaaaacgg
     2641 attccatccg caaagcctca caaactatag ctcggcattt cgcgcttctg ttattccgct
     2701 tttagtcctt tttactatct ttcttttcgt agctattacc aagatcggcg tcaccccaga
     2761 taccggctac tggggcgaac caggtgtagc cattcaaggc tggcattttg ttattgctct
     2821 tctgttggga tgcactgttc taatactttc ctcctctaac tcatcgcttt ttattcgtta
     2881 cttgcctttt gttctttatg ctattgctgc catcctttgg ttaagtgttc cgcttgaaac
     2941 gttggcaaat agcttttatg tctccacaac gccaccgacg aatatgcctt taccatattc
     3001 cgatgctggt ttttacgatt tttctgctca aagcttgtta attggtacag gctacttcgg
     3061 tgggattccg ccacgcccat tgtatgtggt ttttctatcc atcttgcatt tggtatttgg
     3121 gcaaaattat ccagccgtga tcaatgcaca agttttggta ttagcatttt tcccggtcgc
     3181 tttatatttt cttgcgaaga aattgcactc tcccgccgcc ggtgtgaccg ttgccctctt
     3241 tgccattttc cgcgaataca ccggtttgtg gattgcctcg aatacccgtg tcgctaactc
     3301 caaagtcttt accacagact tcccaacagc ttttgctatt gtcattatct gtttggtcat
     3361 tctttggtgg ctggagcgaa gagacttgcg ttctacctta attgcgggtg gagcatttgg
     3421 tattttcttg ctcttccgtt cgcaatctat gctaaccatt ccatttctgt ttcttttggt
     3481 cttgttggta ttcaacttca aatggagtga gtggattaaa tcgggcatta tctttgctgt
     3541 caccatgtta ttggctgtca ttccatggct gacgcataac tacaccatcg caggcaaatt
     3601 ctcatttgat gacccgaacc aggttgcggt gatctatagt caatatgctt ttacaggcaa
     3661 ccttgacctc agccaattca accctgagac cgatagcgtc cgcgaacgga ttatttcatt
     3721 cacacttgaa aacccggcgt acgtggcgaa ctttattacc tctcatttcc taaatactga
     3781 gattggcggt ttgcttgcgc ttccacttat caaaccattc aatggactgc aagaaccggt
     3841 caacttgtac tggatcgaat gggatgggaa ccttgaatgg tacaacgtca ttcttgtctt
     3901 gttctatctg gcaatggttg cactaggcgt aggctctgct tggcgtcgcc tgaagtggat
     3961 ggcgcttgtt ccattggcgt tcaatttggg atacgcggtt tccaatggca tcgcccgttt
     4021 ttctagctgg cgctataaca tgccgattga ctgggtcttt tatttctact ttgccattgg
     4081 cattgtagaa gtgttcagta ttttggctca gttatttgga gcccaggtaa aagaagccca
     4141 aactgaaaca cctatattca agcctattca gttgagtgat tttcgtgtgc aatacctttt
     4201 gcctgtgttt gcatttgtct ttgtgggctc tttgccttgg ctggcaaagg aattggtaca
     4261 accgcgttat atgtcaactc aagatgaatt ggtggcaaag cttgtaacag caggatatga
     4321 ccagaatgag atagatatat tcctgcaaca acccaaagca attctcaatg aaggtaggtt
     4381 gctatttccg cgcatgtact ggcgcggtga agggcttgct tctgcgaacc cgtggcccgc
     4441 atatgccgtg caagatttcc cgcgtattgg gtttattttg atcaacacaa attctcagaa
     4501 tttgatttac ccaaccaaag aagtgttgga ttttccccaa ggtgcagata gcattgtcct
     4561 cgcatgtgcc ggggataatg atttgttata cgtccgcgtg attgcttttg atgatgctac
     4621 ttaccaaaac actccactaa ccgaaccctg tccctgatga agaaccgcct cgcgactcaa
     4681 ctcctaaact ggtaccac
//
LOCUS       DAOQJE010000172         4653 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_10969_length_4653_cov_6.262723, whole genome shotgun sequence.
ACCESSION   DAOQJE010000172 DAOQJE010000000
VERSION     DAOQJE010000172.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 4653)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4653)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4653
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10969_length_4653_cov_6.262723"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..327
                     /locus_tag="PLT08_05925"
     CDS             <1..327
                     /locus_tag="PLT08_05925"
                     /inference="COORDINATES: protein motif:HMM:NF012264.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HNE04034.1"
                     /translation="PADETSIARGKEIYQINCMMCHGETGQGNGTVAAFLVKKKPADL
                     TSEPVQAKSDGSWFLTISNGIWNPNNTLFPERQFSGQMPPLAENLSIRERWDVINYLR
                     TLKASQ"
     gene            340..999
                     /locus_tag="PLT08_05930"
     CDS             340..999
                     /locus_tag="PLT08_05930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE04035.1"
                     /translation="MKDDVRKYIYGAIAVFLVGVLAWVGIVFVSACGVSLSCNRGNTL
                     PDTTPVPTLIPATLPPMTVESNIVAPSDKCYVAGSDLIGAWVDAGSPEADVFEFTDAN
                     GATCEATFEDVQPLFTKANLWYSGSLSCISCHSVDLAVSPAQLDMSSYEGILAGSRRT
                     DDAPKGTDILGGGKWKSSLLYQFIVEAHADVPGHDAALSSDLMVYAGTPLPDVKATPT
                     P"
     gene            complement(1097..1186)
                     /locus_tag="PLT08_05935"
     tRNA            complement(1097..1186)
                     /locus_tag="PLT08_05935"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1148..1150),aa:Ser,seq:gga)
     gene            complement(1235..>2890)
                     /locus_tag="PLT08_05940"
     CDS             complement(1235..>2890)
                     /locus_tag="PLT08_05940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE04036.1"
                     /translation="FALGLAGLWASGNTQSVILNGLLPLTDAAGYYTDALRLQYGSNV
                     SNFTAMRPLYAGLLSFFLWISNNNLMIAVSIFTAITGFSCYLACKEIQKTHGAEVAVF
                     FLILTFLYYRHHSGTTMSESFGVPVSLLGVALLWKGIYSKNEGTTLFGIGMIALALNI
                     RPGAMFVLPGLLLWGGWIFRKEKKFSFKFFISGLTVVLSVFYINSRMIDFVAGPSGVA
                     FENFSWAFYGLSSGGKSWTYIFEAHPELRLLQDSEVTPTIYKLAFKQILNNPSLMIKG
                     SFFYWRMFFSDSWYNAYAFVAGENYWISEIARWGMYILGALGIYKLLKNYQDPYTSLA
                     GITAVGVLASVPFVPPTDAYRVRLYAATIPFFILLPGMGVSLLLDAVPQRLLVPKFTF
                     LNKSSTSVIFSIVLILTLLLSPIYIKKTGTVPTFSQTSCPASLDSFLIRYDDGSSFTV
                     YRESQVFIDQMPDFHMSTFRRNAHSLPDMNLITTLENIEPPKTITYSIDLLTNNQAII
                     IAEPELLPVSGVYLLLCGKFIYEPNFTPYSFFYVEQVFSVEGH"
     assembly_gap    2891..2990
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(3110..4405)
                     /locus_tag="PLT08_05945"
     CDS             complement(3110..4405)
                     /locus_tag="PLT08_05945"
                     /inference="COORDINATES: protein motif:HMM:NF017760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNE04037.1"
                     /translation="MTSEQQVPENWAIRFFTIFAGQAFSLFGSALVQFALIWHLTQKT
                     GSATVLATASLFGMLPQVLLGPIAGTIVDRGNRRLIMILSDSLIAISTLLLAYLFWSG
                     AAEIWHIYVALTIRSAGGAFHYPAMASSTSLMVPDKHLSRVAGANQTLQGLISIVAPP
                     VGALLVAAMSTQNILMIDVITAIMGVTPLLFFTIPQPPRKAAQTATQTSFLKDLGAGF
                     KYMASWPGLLMLGLLATVLNFLLAPLTALMPLLVTKYFKLGALELGSLDSFFGIGMIA
                     GGITLGVWGGFKKRIVTSLSGIILTSFSILAIAGAPSNAFWMVVAAMAFMGFMMPMVN
                     GPIHALFQSVVEPDMQGRVMSLISSVAQAMMPIGFILAGPITDAISFQAWFWVAGILN
                     LLIGVSGFFIPAVINIEENRKAYHPAENNESTPITEFSN"
     gene            complement(4402..>4653)
                     /locus_tag="PLT08_05950"
     CDS             complement(4402..>4653)
                     /locus_tag="PLT08_05950"
                     /inference="COORDINATES: protein motif:HMM:NF036989.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE04038.1"
                     /translation="LEVQQAVTRYSFGVGAFFHSRADFEKYKAKGDKIKIDVIGIYHV
                     PDDVSVALALEVVESIKVYGIFQARSELKDALQKAGKIR"
BASE COUNT         1365 a          958 c         1030 g         1200 t
ORIGIN      
        1 cctgcagatg aaacctccat cgcccgtgga aaagagatct atcagatcaa ctgcatgatg
       61 tgccatggcg aaactgggca ggggaacgga accgtggcag cattcctcgt aaagaagaag
      121 cctgctgacc tcactagtga acctgttcaa gctaaatcgg atggtagctg gttcctgacg
      181 atctctaacg gtatttggaa tcccaacaac actcttttcc cagaacgaca gttctccggg
      241 caaatgccac ctttggctga aaatctcagc attcgcgagc gttgggatgt gatcaactac
      301 ctccgcacgc taaaagccag tcagtaggag atgcatgcaa tgaaagacga tgttcgaaaa
      361 tatatttacg gtgcaatcgc cgtatttctg gtaggcgtgc tggcttgggt agggatcgtg
      421 tttgtgagtg cttgtggtgt ttctctttcc tgcaaccgcg gcaatacttt gcctgatacc
      481 acccctgttc caaccttgat tccagccaca cttccgccta tgactgtgga atccaacata
      541 gtggcaccct ctgataaatg ctatgttgct ggctccgatt tgatcggcgc ttgggtggat
      601 gcaggctctc cagaagcaga tgtttttgag ttcacagatg ccaatggtgc aacctgtgaa
      661 gctacttttg aggatgtgca gcctttattc actaaagcta acctttggta ttccggctct
      721 ctctcctgca tttcctgtca ctctgttgat ttggctgttt ctcccgccca attggacatg
      781 agcagctatg aaggcatcct tgctgggtca cgccgtacgg atgatgcacc taagggtacc
      841 gatatccttg gcggtggaaa gtggaaatcg tccctgttat atcagttcat tgtagaggca
      901 catgccgatg tccctgggca tgatgcggct ctatcctctg acctgatggt atatgcagga
      961 actccgctac ccgatgtaaa agctacacca acaccatagt atccagaata acaaaaaagg
     1021 acctgatttc atgatcaggt ccttttttgt tagagaaaat ttgataaaaa gaaagcccct
     1081 cattgagggg catggctggc ggagagggcg ggattcgaac ccgcgaacct ttgtgggtta
     1141 cacgctttcc aagcgtgcgc gctaagccag actacgcgac ctctccaaac taacagcggg
     1201 cagtattata ccatgcgttt ctatattggc aagtttaatg cccttcaact gaaaagactt
     1261 gctcgacgta aaagaaggag tatggagtga aattaggttc ataaataaat ttgccgcaaa
     1321 gaagcaggta tacacctgaa acaggcaata attcaggttc ggcaataata atagcctggt
     1381 tgttcgttaa caagtcgatg gagtaagtga ttgttttagg cggttctata ttttctaagg
     1441 ttgttatgag attcatatct ggcagactat gagcattccg acgaaaagta ctcatatgaa
     1501 aatctggcat ttgatcaata aacacttggc tctctctata aacagtgaaa gagctaccat
     1561 catcatacct tatgagaaaa ctatctaaac tagccgggca acttgtttgt gaaaatgtag
     1621 gcacggttcc agtttttttt atatatattg gtgatagaag aagtgtaagt attaatacaa
     1681 ttgagaagat aacgcttgtt gagcttttat taagaaaagt aaatttaggg actaagagtc
     1741 gttgaggaac tgcatcgagc aatagagaga ctcccattcc tggtaataga atgaaaaaag
     1801 gaattgttgc cgcgtaaagc cggactctat atgcatcagt cggcgggacg aagggtactg
     1861 aagctagtac tcctactgct gtaattccag ctagacttgt atacggatct tgatagtttt
     1921 ttagtaattt ataaatccct aatgctccaa gaatatacat gccccatcgt gctatttcac
     1981 tgatccaata gttttcacca gctacaaaag cataggcgtt gtaccatgaa tcagagaaaa
     2041 acattcgcca gtaaaaaaat gaacccttaa tcataagaga tggattgttc agtatttgtt
     2101 taaaagcgag tttataaatg gtaggtgtta cttctgagtc ttgtaggaga cgtaattcag
     2161 ggtgtgcttc aaaaatataa gtccaagact tgccgccgga agataagcca taaaatgccc
     2221 acgagaagtt ttcaaatgct actccacttg gaccagcaac aaaatctatc atgcgactat
     2281 ttatataaaa cacagataaa actacagtta gtccagagat aaagaatttg aaagaaaact
     2341 ttttttcttt tcgaaatatc caacctcccc atagtaaaag tccgggtaga acaaacatag
     2401 ctcctgggcg aatattcagg gctaaagcaa tcatccctat tccaaataaa gtagttcctt
     2461 cattttttga ataaatcccc ttccaaagta aagctacacc aagcaagctt actggaactc
     2521 cgaaactttc cgacatggta gttccactgt ggtggcgata atatagaaag gtcagaatta
     2581 agaaaaatac agcaacttca gccccatgtg ttttttgaat ctctttacaa gctaagtaac
     2641 aagaaaaacc tgtaattgcc gtgaaaattg atacagcaat cattaggttg ttattcgata
     2701 tccaaagaaa aaaagataat aatcctgcat acagcggacg catcgcagta aagttggaaa
     2761 catttgatcc atattgcaat cgtagtgcat cagtataata accagcagca tctgtaagcg
     2821 gaagcagtcc atttaaaatt acagactgag tgttccctga tgcccataat ccagctaatc
     2881 ccaatgcaaa nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2941 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn ttgattttta
     3001 ttaacataga atagttatta taaatgaatt atctatgcct taaatgagtt acaggtcgca
     3061 gcataaggcg cgacctgtaa ccatcatctt ggaggatgaa tttaataaat taattgctaa
     3121 attctgttat aggtgttgac tcgttatttt cagcgggatg ataggctttt cggttctctt
     3181 ctatattgat aactgctggg atgaaaaaac cgctcacccc aataagcagg ttaagaatac
     3241 ctgccaccca gaaccatgct tgaaaactaa ttgcatccgt aatgggacct gcaaggataa
     3301 aaccgattgg catcatggct tgagcaacgc tgcttatcaa tgacatgact cttccttgca
     3361 tgtcgggctc tacaacagac tgaaataagg catggatcgg tccattaacc atgggcatca
     3421 taaatcccat gaatgccatg gctgcaacca ccatccagaa ggcatttgaa ggggcacctg
     3481 caatggcaag gatagaaaaa ctagtgagga tgatacccga gagggaagtg acgatccgtt
     3541 ttttgaaacc accccaaacc ccaagggtaa ttccgccggc aatcatgccg atgccgaaga
     3601 atgagtcgag actgcctaat tcgagagcac ctaatttgaa gtatttcgta actaacaatg
     3661 gcatgagcgc tgtgagggga gcgaggagga agttcaatac agttgccagt aaacctaaca
     3721 tgagtagacc aggccaggat gccatatatt tgaaacctgc accgaggtct ttaagaaagc
     3781 tggtttgtgt agcggtttgc gcagctttac ggggtggttg tggaatggtg aagaaaagca
     3841 acggagtgac acccataatg gcagtaatta cgtcaatcat caaaatattt tgtgtagaca
     3901 tggctgcgac aagcagagcg cctactggtg gagcaacgat gctaataagc ccttgcaagg
     3961 tttggttggc accggctaca cgcgagaggt gtttatcggg aaccattaac gaggtagatg
     4021 acgccattgc ggggtagtgg aaagctccgc ctgcagatcg gatggttaag gctacataga
     4081 tatgccaaat ttctgcagcg ccagaccaga ataagtaggc gagaagaagc gtagagatcg
     4141 caatcagtga gtctgatagg atcatgatca agcgacggtt gcctcgatcc acaatggttc
     4201 ctgcaatggg tcctaaaagt acctgtggaa gcatcccgaa taaagatgcg gtagcaagta
     4261 cggttgcaga acctgtcttt tgggtaaggt gccagattaa tgcaaactga actaatgcag
     4321 agccgaataa tgaaaaagcc tgacctgcaa agatggtgaa gaaacggata gcccagtttt
     4381 ctggaacttg ttgttcgctt gtcatctgat tttccctgct ttctgaagag cgtcttttaa
     4441 ttcactacgc gcttggaaga tgccatacac tttaatggac tcgacgactt ccagcgcaag
     4501 agctacggaa acatcatctg gaacatggta tatcccaatg acatcgattt tgatcttatc
     4561 gcccttggct ttatattttt caaagtctgc acggctatga aagaatgcac cgacaccaaa
     4621 tgaatagcgg gtgactgctt gctgtacctc aag
//
LOCUS       DAOQJE010000173         4653 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_10970_length_4653_cov_6.149413, whole genome shotgun sequence.
ACCESSION   DAOQJE010000173 DAOQJE010000000
VERSION     DAOQJE010000173.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 4653)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4653)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4653
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10970_length_4653_cov_6.149413"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..761
                     /locus_tag="PLT08_05955"
     CDS             <1..761
                     /locus_tag="PLT08_05955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE04039.1"
                     /translation="LVILPAVNFFNSEFIQDPAERLQFSLTQGLKFSALILLFAWIGV
                     GLSSLLTLIKDGRSRKLISWVVYLGFLFFCIITPILGVVFVYGVSYAGSSWQKFPTPP
                     ETPKAIAAAGYSTVVIETEEGHYFYCDTSQLELCWQPENKPSSLIIPVSYGSVEQIEG
                     KPIVPAPGRVVSLVGVDHQDGPNHTEIYYAILKNGSIWYLQLDQSSAGLLAGLSSLIA
                     IPIVFVSSMLFIGMVLAAFLHWLANRIWREKEAA"
     gene            complement(797..1210)
                     /locus_tag="PLT08_05960"
     CDS             complement(797..1210)
                     /locus_tag="PLT08_05960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE04040.1"
                     /translation="MHRHKRYRYGPPLFPRPFRGWIWMLLIAFMMFGGRGNWWPGILI
                     LVGVMFLFSSLFRDQRPPEPPPPPMQHPPFDMSPPPPPRRADTPPSEPVRRADLLPAT
                     CPNCGGPVRSNDLKWTSSHSVACSYCGSNIETRKE"
     gene            complement(1261..1665)
                     /locus_tag="PLT08_05965"
     CDS             complement(1261..1665)
                     /locus_tag="PLT08_05965"
                     /inference="COORDINATES: protein motif:HMM:NF014608.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HNE04041.1"
                     /translation="MASKTVYLNWDSESKFVLNDRDNYQLRVNGAGNIGSADLLPMSL
                     IGCSAYDVVEILEKQRQELHQLKVTAEAQQDDTPPWKFRKIHIRYQVIGKGIDLEKAK
                     KAIQLSEEKYCSVYATLRDVIEITHEVEVLEA"
     gene            1719..>2047
                     /locus_tag="PLT08_05970"
     CDS             1719..>2047
                     /locus_tag="PLT08_05970"
                     /inference="COORDINATES: protein motif:HMM:NF019559.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysoplasmalogenase family protein"
                     /protein_id="HNE04042.1"
                     /translation="MVIFLVIAFVFAGLESLALYRNWVKVEWVAKPAVMVCLLIWLFT
                     SVGLEGALLWFGLGLLFSLVGDVLLMISLDTLFLAGLVAFLFAHVFYVIGFNIPLPAI
                     SAWSVILA"
     assembly_gap    2048..2147
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            2283..3254
                     /locus_tag="PLT08_05975"
     CDS             2283..3254
                     /locus_tag="PLT08_05975"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR01251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0002189 - ribose phosphate
                     diphosphokinase complex [Evidence IEA];
                     GO_function: GO:0004749 - ribose phosphate diphosphokinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate pyrophosphokinase"
                     /protein_id="HNE04043.1"
                     /translation="MSRPIPADEVRIFSGTSNLPLAKSIAAELDIPLEKTIVKKFSND
                     NLYLQLGASVRYRRVYIVQSLTPPVNDNLMELLMMLDIARDAAASEIHAIIPYYSFGR
                     SDKKDAPRISITARLVADLLRTSGATHVMSMMFHSPQVHGFFRVPTDPLSSRPVFQQY
                     LENRDMSGMIIVSPDMGQAKSAARFAKSLSLPVAAGNKERVSDTEVRISGLVGNQVKG
                     HKRALIYDDEIATGGSIAELTRLLVNEGVEEIMVMCTHGVFTRGGLEKLAAIPQIQEI
                     VTTDTVDIPAEKRHPKLTVLSVAKVFADSIRHNFNRQSIGDLFVYGE"
     gene            complement(3305..4303)
                     /locus_tag="PLT08_05980"
     CDS             complement(3305..4303)
                     /locus_tag="PLT08_05980"
                     /inference="COORDINATES: protein motif:HMM:NF025030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HNE04044.1"
                     /translation="MAELTFQNVLDHLLDSKKDIPAGHLAYYSDLDPKSLRLFLDVWS
                     SVPQKRKLLLLDTLVSHLDEDTIVSYEDIGKALLDDPDAEVRARAMGLLAESDDPKLI
                     DTLLKILKSDSELAPRVKASTLLGEFVLMGELEELDEARLRKIEEALIAIIRSDENPS
                     LRRAAVEAFGYSGRDEAVAILESAYEREDPLWVASALRAMGRSHDNRWDDSVVSKLLD
                     PDPRVRYAAAEAAGELTIEEAGPIMLKMLEDEEEDDNVTMAAIWSLSQIGGEDARAYI
                     LNMLDSSDDDDVTEFLEDALENLDFNDELNKFDLLSLDEDDDLSEFDEDDLDDDDK"
     gene            complement(4307..>4653)
                     /locus_tag="PLT08_05985"
     CDS             complement(4307..>4653)
                     /locus_tag="PLT08_05985"
                     /inference="COORDINATES: protein motif:HMM:NF014244.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HNE04045.1"
                     /translation="GIYDLFNYSVQEDVSSRQEAAQVQTSGGGSAPKPTNFTLNLIGV
                     LSVSLGVFNLFPIPALDGGRILFTLPEILFKKRIPTEWENMVNGVAMLLLIGLMLFVN
                     IMDFVNPANLTP"
BASE COUNT         1040 a         1059 c         1211 g         1243 t
ORIGIN      
        1 tgcttgttat tttgcctgct gtaaatttct ttaattccga atttatccaa gatcctgctg
       61 agcggcttca attttcgttg acccaggggc ttaaattttc tgcgctcatt cttttgtttg
      121 catggatcgg cgttgggctt agtagtttat tgacattgat caaagatggg cgatctcgta
      181 agctcatttc gtgggtggtg tatcttggtt ttcttttctt ctgtatcatc acgccgattc
      241 ttggcgttgt atttgtgtac ggtgtttcat atgctggttc tagttggcaa aaattcccca
      301 ccccgcctga aacaccaaag gcaatcgctg cagccgggta tagtacggta gtgattgaaa
      361 cagaagaggg acattatttc tattgcgata cgtctcaact tgagttgtgt tggcagccag
      421 agaataaacc gagtagccta atcatccctg tgtcttacgg ctcagtagaa caaattgaag
      481 gcaaaccaat tgtccctgcc cctgggagag ttgtgagttt ggtaggcgta gatcatcaag
      541 atggtccaaa ccatactgaa atctattatg cgattctaaa gaatggcagt atttggtatt
      601 tgcaattgga tcagagttca gcaggtttac tggcaggtct ttcttctctt attgcaattc
      661 ctattgtttt tgtgagcagc atgctcttta tagggatggt gcttgctgca ttcttgcatt
      721 ggttggcaaa ccgcatctgg cgcgagaaag aagccgcata aagaaaaagc tctgggttca
      781 aaacccagag ctttttttat tcttttctcg tttcaatgtt cgacccgcaa tacgagcagg
      841 cgacggagtg agaactggtc cacttcaggt cgtttgagcg gacgggacca ccacagttgg
      901 ggcaggtggc gggcaacagg tctgcgcggc gcaccggctc acttgggggc gtgtccgctc
      961 gccgcggggg aggaggcgga gacatgtcaa atggcgggtg ttgcatgggc ggcggtggag
     1021 gttcgggcgg gcgttggtct cgaaagagag agctaaagag aaacatgacg ccaaccaaga
     1081 tgagaatgcc gggccaccaa ttgcccctgc cgccgaacat catgaaagcg attaagagca
     1141 tccagatcca gccgcgaaag ggtctaggga agaggggcgg tccataacgg taacgtttat
     1201 gtctgtgcat gaaatatcct ttgggtacgc atactatacg cgccaccctc attgaagttg
     1261 ctatgcttcc aacacttcta cctcgtgcgt gatctcgatc acatcgcgca gggtggcgta
     1321 caccgagcaa tatttttctt cgctgagttg aatggctttc tttgcctttt caaggtcgat
     1381 gcctttgcca atgacctgat accgaatgtg gatctttcga aacttccaag gtggtgtgtc
     1441 atcttgctgc gcctctgccg tgaccttgag ctggtgcagt tcctgacgct gtttctccag
     1501 aatttcgacc acgtcatatg ccgaacagcc aatcagcgac atgggtaaca aatcggcgga
     1561 gccaatattt cccgcgccat tgacgcgtaa ttgataattg tctctatcgt tgagtacaaa
     1621 tttggactct gagtcccagt tgagatatac agttttcgat gccatatccg cagtatagca
     1681 tgggatgcct tcgtggtagg atagagtagg agggcactgt ggttatcttt ttagtgattg
     1741 cgtttgtatt tgcagggttg gaatctctgg cattgtatag aaactgggtg aaagtggaat
     1801 gggttgccaa accggcggta atggtttgct tactcatctg gttattcact tctgttggat
     1861 tagaaggggc gttgttatgg tttggtttgg ggttgctctt ttctttggtg ggtgatgtgt
     1921 tattgatgat ctcgctcgat acgttgtttt tagcgggact ggtggcattc ttatttgcgc
     1981 atgtgtttta tgtgatcggg tttaatatcc ctttgcctgc catctcagcg tggagtgtga
     2041 ttcttgcnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2101 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnttt aattatgggc
     2161 gtatctataa catcgccttg tatcatcttg gtcagatcat gttgatctcc ggggtgatcg
     2221 tgcagtatca ccgctgattt gtgtcataat cggaatacaa ttcaaggtat atggagaatc
     2281 atatgtcgcg tcccatcccc gctgatgaag ttcgcatttt cagtggcacg tctaatttgc
     2341 cgcttgccaa aagcattgcc gcagagctgg atattccatt agaaaagacc atcgtgaaga
     2401 agttcagtaa cgataatttg tatttgcagc tcggtgccag tgtgcgctat cgccgcgtgt
     2461 acattgtcca gtcgctcacg ccgccggtca acgataattt gatggaactg ttgatgatgc
     2521 tcgatattgc gcgcgatgcc gccgcttctg agatccatgc cattattcct tattattctt
     2581 ttgggcgttc cgataaaaaa gacgcgccgc gcatctccat cacagcccgc ctcgtcgcgg
     2641 acctgctcag aacctctggc gcaacgcatg tgatgagcat gatgttccac tcgccgcagg
     2701 tgcatgggtt cttccgtgtg cctacagacc ccctgagcag tcgcccggtc tttcagcaat
     2761 atttggaaaa ccgcgatatg agcggcatga tcatcgtttc gcccgatatg ggtcaagcca
     2821 agtctgcggc gcgctttgcg aagagcttaa gtctgcctgt ggcggcagga aataaagaac
     2881 gcgtctccga taccgaagtg cgcattagcg gacttgtcgg taaccaagtt aaagggcaca
     2941 aacgcgcttt gatctatgat gatgagatcg ctacgggtgg ctccattgct gaattgacgc
     3001 gtttgctggt gaacgaaggc gtggaagaaa tcatggtgat gtgtacccat ggtgtcttta
     3061 cccgcggcgg gttggagaaa ctggcggcga ttccgcaaat tcaagaaatt gtaacgaccg
     3121 ataccgtcga tatccctgca gagaaacgtc accccaagct gaccgttctt tcggtggcaa
     3181 aggtctttgc agactccatt cgccataact tcaatcgaca gtcgattggt gacctgttcg
     3241 tgtacggcga ataactctca gcgcgaacaa aaagacactc ccttactggg agtgtctttt
     3301 tgttttattt atcatcatcg tcgaggtcgt cttcgtcgaa ttcagaaagg tcgtcatctt
     3361 catcgagcga gagcaggtca aacttgttta gctcatcgtt gaagtctaaa ttttcgagcg
     3421 catcttcgag aaattccgtt acatcgtcat catcggagct atcgagcatg ttgaggatgt
     3481 aggcacgcgc atcttcgccg ccgatctggc tcagagacca aatggctgcc atggtcacgt
     3541 tatcgtcctc ttcttcatct tcgagcatct tcagcataat aggacccgct tcttcgatgg
     3601 tcagttcacc ggcggcttcg gcggcagcat agcggacgcg tggatcgggg tcaagcagtt
     3661 tgctgaccac gctatcatcc cagcggttat cgtgcgaacg tcccatggcg cgcagagcag
     3721 atgccaccca aagcggatct tcacgctcat acgctgattc aaggatcgcc accgcctcat
     3781 cacgaccaga gtagccgaag gcttccacgg cggcgcggcg caggcttgga ttctcgtcgc
     3841 tgcgaatgat ggcgatcagc gcttcttcga tcttgcgcaa acgggcttca tcaagctctt
     3901 cgagttcacc catcaacacg aactcgccaa gcaacgtaga ggctttcaca cgcggagcaa
     3961 gctcgctgtc agatttgaga atcttcagga gggtatctat aagcttgggg tcgtctgatt
     4021 cggcgagcag acccatagct cgggcgcgga cttccgcatc cgggtcatcc agaagcgctt
     4081 tgccgatgtc ttcatacgaa acaatggtgt cttcatcgag gtgagagacc aaggtatcta
     4141 atagaagcaa tttacgcttt tgcggaacac tgctccaaac atccaaaaag aggcgcaaag
     4201 attttggatc caggtctgag taataggcaa ggtgacccgc aggaatatcc tttttggaat
     4261 caagcaggtg atcgagtacg ttttgaaaag taagttcagc cataagttac ggagtgaggt
     4321 ttgcagggtt gacgaagtcc atgatgttga cgaacaacat caagccgatc agcaggagca
     4381 tggcaacacc gttcaccatg ttctcccatt cggtagggat gcgcttcttg aaaagaattt
     4441 cgggcagggt aaagagaatg cgcccgccat cgagtgcagg gatggggaag aggttgaaga
     4501 cgcccaacga aacactgagt acgccaatga gattgagcgt gaagttggtt ggtttgggag
     4561 cgctgccgcc accacttgtt tgtacttgcg cggcttcctg tcggctcgat acatcttctt
     4621 gcacggaata attgaaaagg tcgtagatac ctt
//
LOCUS       DAOQJE010000174         4625 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_11058_length_4625_cov_6.767177, whole genome shotgun sequence.
ACCESSION   DAOQJE010000174 DAOQJE010000000
VERSION     DAOQJE010000174.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 4625)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4625)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4625
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11058_length_4625_cov_6.767177"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..345
                     /locus_tag="PLT08_05990"
     CDS             <1..345
                     /locus_tag="PLT08_05990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE04046.1"
                     /translation="NGGSYYRFALACDGTVRAERVSNSVRLVLQKPIPSGDVPPGAPG
                     NVRIGVWIVGNEMRLFLNGRFQFSINDPSFPIGTVGVFVRSAGETAAIVSFSDLVIQE
                     VDYIPPTPTATP"
     gene            461..1432
                     /locus_tag="PLT08_05995"
     CDS             461..1432
                     /locus_tag="PLT08_05995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE04047.1"
                     /translation="MLSSSDFLHLPYTPDLTEAGIAYALRSLAYSYERDGRSPYGRLR
                     RLVANVAVELAFRRYLTQEHVSFEVKASTPFTDRERFDVVLNGHRCDLKPFLISHREQ
                     ILQIRKNPAVLLDAQALVPSDSHAGDGHSYNDIYLFAFMNGLVAASQADLQKAIEKNQ
                     PHYLVYVPSMLLRKPIHWKPLGTLTLRSESAEELIVEVNGQDEAREMKREVISLSPGV
                     NVKLHEVFYSITSLHVRRVPEARIRIHCDALNETLVIAPHDWSNIWVYGMDIFMVGYA
                     TYEEMSQRATLLLPNSRTFQYERTHVKNLSLLVSKLKPIQKLWAAVS"
     gene            1484..2335
                     /locus_tag="PLT08_06000"
     CDS             1484..2335
                     /locus_tag="PLT08_06000"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: protein motif:HMM:NF013670.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HNE04048.1"
                     /translation="MESTNEPVSQEPVVQEPAMHETPVRRRQPKPRGINPFLSTLGIA
                     VLIATLFTAWTPNSIFTNNLSDQLRTLLTPQSAIQSGASTPLPQIRIGIVAGHMGNDS
                     GAVCYDDNGEPDLTEADVNLSIATLVQRSLTERGFQVDLLNEFDTRLNGYRAAALVSI
                     HNDSCDYINDEATGFKVAASLETRDVNRAQRLTACLTDRYQNLTGLFFHANSITPDMT
                     QYHAFSEIDPNTITAIIETGFLYLDREILTKQTDVVAEGVTQGILCFVNNESVLPNPV
                     NLPTILP"
     gene            2332..3009
                     /locus_tag="PLT08_06005"
     CDS             2332..3009
                     /locus_tag="PLT08_06005"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015679.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_function: GO:0071972 - peptidoglycan L,D-transpeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HNE04049.1"
                     /translation="MTSRITGMIFGLLIFLAGAIAWTVTTSEALAPIPNVTPTYSKPF
                     AVAELLKPTYTPLPPPTLAPTQEPSLTPQALVEAVIVDSPERVNAAAEEQAPVNYSGG
                     KKILVDISEQHMYVYDGEQIVYSFVASTGMNNATRTGTFSVLDKIPNAYGATWNIWMP
                     NWLGIYWAGSLENGIHALPILPSGATLWAGYLGTPISYGCVVLDTYDAQVLYDWAEVG
                     VPVEIQW"
     gene            3095..4021
                     /locus_tag="PLT08_06010"
     CDS             3095..4021
                     /locus_tag="PLT08_06010"
                     /inference="COORDINATES: protein motif:HMM:NF015679.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HNE04050.1"
                     /translation="MNNRLTNSQAPAQTGGKSPKNGNPAKGPAKKRNLILPIVLVITG
                     CVVFGFAAWSAVTSPVMASILNRGSNSAEFQQVTPGQSFAQVYIAKPTYTPMGLEPVA
                     QQVVVQPTVTFTPTSQAELAVVQPTDPPTIEAAAEVVVQPTATTESVAVVSTGEAVAQ
                     AQIIADTPTSEYVAPPTAVANAPAPVSSPGNGVRWFDVDLSDQRMYAYEGDTLVRTFV
                     VSTGTWQTPTVTGRFKVWIKLKSAPMSGPGYYLPDVPYIMYFYGDYGIHGTYWHNNFG
                     VPMSHGCVNLSIPDAEWAYNFASVGTVVNVHD"
     gene            4120..>4625
                     /locus_tag="PLT08_06015"
     CDS             4120..>4625
                     /locus_tag="PLT08_06015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE04051.1"
                     /translation="MVPPLNFDSDDPFDLQQGRVTVRTVWVYDKPTLEATKVKLLQRD
                     AIFNITNTAVTEDIDSHNRIWYQVGTDGYVPSGNIQPVRTMLSQPYTANIPIEGLLAE
                     VCVPYTDAFVEPSTESKVGYRIYYETTHWVKALVAGKTDGQPWYQIRDDKWDELYYVR
                     ADHIRIMTP"
BASE COUNT         1148 a         1255 c         1079 g         1143 t
ORIGIN      
        1 aatggtggct cctactatcg tttcgccctc gcctgcgatg ggaccgtccg tgctgaacgt
       61 gtgagcaata gcgtaaggct tgtgctgcaa aaacccatcc ccagcggaga tgtgcccccc
      121 ggcgcaccgg gcaatgttcg cattggagta tggatcgttg gcaatgaaat gcgcctcttc
      181 ctaaacggac gtttccaatt cagcatcaac gacccgtcgt tccctattgg taccgtcggc
      241 gtattcgtcc gctcggcagg agaaaccgcc gccatcgttt cgttttctga ccttgtcatt
      301 caagaggtgg attacattcc ccctacccca actgctacac cgtagcaatt gacgattttt
      361 gatttgagat ttacgattag aacagtagtg ggaaacgcat caaacatcaa caccataccc
      421 acaacacata ttggtacttg gaatactggt cactgatact atgctatcgt cttccgactt
      481 cctacacctt ccttacacgc cagacctcac agaggcaggc attgcctatg cattgaggtc
      541 actggcatat tcttatgagc gcgatgggcg ttctccatat gggcgtttgc gccgtctggt
      601 ggcaaatgtt gccgtagagt tggcatttcg gcgttatctc acacaggaac acgtttcatt
      661 tgaagtaaaa gcctccacac cgtttacaga ccgagaacga ttcgacgtgg tcttgaacgg
      721 tcaccgctgc gacctcaaac cttttcttat ctcccatcgc gaacagattc tacaaatccg
      781 taaaaaccct gccgtcttgt tagatgctca agccttagtg ccttctgact cacatgccgg
      841 agatggacat tcctacaacg atatctatct ctttgccttc atgaacggac tcgtcgcagc
      901 gtcacaagca gacttgcaaa aagccatcga aaagaatcag ccgcattatt tagtctatgt
      961 gccatcgatg ttacttcgta aacccataca ttggaaacca ctcggcacgt taactctgcg
     1021 ttctgaatca gcggaggaat taatcgtcga agtcaatggg caagacgagg cgcgggaaat
     1081 gaagcgcgaa gtcatcagcc tttcgccggg cgtcaatgtc aaattgcatg aggtcttcta
     1141 ttcgatcacg tccctgcatg tccgccgcgt gcccgaagcg cggattcgta tccactgcga
     1201 cgcgttgaac gagacactcg tgattgcgcc acatgactgg tctaatattt gggtctacgg
     1261 catggatatt ttcatggtgg gatatgccac ctacgaagag atgagtcaac gtgccacact
     1321 gttactgccc aactcgcgga cattccagta cgaacgcacg catgtcaaaa acctttcgct
     1381 gctggtctcc aagctaaagc ccatccaaaa gttatgggcg gcggtttcgt agcaatctgt
     1441 ttttcatttg tatccaagca aaataaggta atattattta gctatggaat ctacaaacga
     1501 acccgtctca caggaacctg ttgttcagga gcctgctatg cacgaaactc cagttcgacg
     1561 caggcagccc aaaccgcgcg gcattaaccc ttttctttcc acgctgggca ttgcggtctt
     1621 gatcgcaact cttttcaccg cttggacccc caacagtatt tttacaaata atctcagcga
     1681 tcaactgcgc acattgctca cgccgcaatc agctattcaa agcggcgcat ccacgccttt
     1741 gccgcaaatt cgcattggca ttgttgccgg gcacatgggc aatgactcgg gggctgtttg
     1801 ttacgatgac aatggcgaac ccgatctaac agaagcggat gtcaacttat cgattgcgac
     1861 cctcgtacag cgcagcctca ccgagcgcgg ctttcaagtg gacttgctca atgaattcga
     1921 tacgcgtttg aatggctatc gtgcggcagc attggtgtcc attcataacg actcttgcga
     1981 ttacatcaac gatgaagcga ccgggttcaa agtagcggct tcgcttgaaa ctcgcgatgt
     2041 aaatcgtgca cagcgcctca ccgcctgcct cacagaccgt taccaaaacc tgaccgggct
     2101 tttcttccat gccaacagca tcacgcccga tatgacccaa tatcacgcct tctcagaaat
     2161 tgacccgaac accatcactg ccatcatcga aaccgggttc ttatacctcg accgcgagat
     2221 tctcaccaaa caaactgatg tggtagcaga aggcgtaacg caaggcattt tgtgcttcgt
     2281 caacaacgaa agtgttttac ccaaccctgt taacctgcca accatcctcc catgacctct
     2341 cgcatcaccg gcatgatttt tgggctgctt atttttcttg cgggcgccat cgcatggact
     2401 gttacaactt ctgaagctct tgctccgatt ccaaacgtca ccccaaccta tagcaaaccc
     2461 tttgccgtag cagaattgtt gaaaccgaca tacacgcctc tccccccgcc gacgcttgct
     2521 cctacccaag agccttcact tacgccgcaa gcattagttg aagctgtgat cgtggactct
     2581 cccgagcgcg tcaacgcagc agcagaagaa caagcaccgg tcaactacag tggcggcaaa
     2641 aagattctcg tcgacatttc tgaacagcat atgtacgtct atgatgggga acaaatcgtc
     2701 tacagcttcg tcgcttctac aggcatgaac aatgccaccc gcaccggaac ctttagtgtg
     2761 ctcgataaaa tccccaatgc ctacggtgct acatggaata tctggatgcc caactggctt
     2821 ggtatttatt gggcgggtag cttagagaat ggcatccatg ccttgcccat cctgccgagc
     2881 ggagccaccc tctgggctgg ctatttgggg actccaattt cgtatggttg tgtcgtcttg
     2941 gatacatacg atgcacaagt gctgtatgac tgggcagagg tcggcgtgcc tgtcgaaatt
     3001 cagtggtgaa attggaatct ttttgtttat aatcttatat agttcgtttg ttttgtaatt
     3061 gtccttattc gttgatgcct caaaaggtga ccccatgaac aaccgtctca caaattcaca
     3121 agcgcccgct caaacgggtg gcaaaagccc aaagaacggc aacccggcga agggtccggc
     3181 aaagaagcgg aatctcatcc tgccgattgt cttggtcatt acagggtgtg ttgtttttgg
     3241 cttcgcggca tggtcggcag tcacatcgcc tgtgatggca tcgattttga atcgcggctc
     3301 aaatagcgca gaattccagc aagtgacacc cgggcagtct tttgcgcagg tgtatattgc
     3361 caagccaaca tataccccta tggggctgga accggtagcg caacaagtcg ttgtgcaacc
     3421 aacagtgaca tttacaccca cctctcaagc agagttggca gttgtgcagc ctacagaccc
     3481 accaacaata gaagcggcgg cagaagtggt tgtgcaaccc acggctacta ctgaatcggt
     3541 tgcggttgta tctacaggcg aagcagtagc tcaagcccaa atcatcgcag atacaccaac
     3601 ttcagaatat gttgcgccac ctacggctgt tgcaaacgca ccagcaccgg tcagctcgcc
     3661 gggcaatggg gtacgttggt tcgatgtgga cctttcggat cagcgcatgt acgcttacga
     3721 aggcgatacc ttggtacgga cctttgtggt ttctacaggc acctggcaaa cccccaccgt
     3781 aaccggacgt ttcaaagttt ggatcaagct aaaatctgcg ccaatgtcgg gtccgggtta
     3841 ctacttgccg gatgttcctt acattatgta tttctatggc gactatggaa ttcacggcac
     3901 ctactggcat aataactttg gcgtgcccat gagccacgga tgcgtgaacc taagcattcc
     3961 cgatgcagaa tgggcgtata actttgcttc agtgggcact gtggtgaacg tgcacgatta
     4021 atggtgagtt gtcaccctgt gtgactctgt tctttagaaa tgaagacttt atcaagaaga
     4081 gatttcctaa agttgagcgg gctagggcta gccagtttca tggtgccacc gctcaatttt
     4141 gattctgatg acccattcga cctgcaacaa gggcgcgtca ccgttcgcac agtatgggtg
     4201 tacgataaac ctacgctcga agcaacaaag gtcaaactgc ttcagcgcga tgcaatcttc
     4261 aacatcacca ataccgcagt gaccgaagat atcgactcac acaatcgcat ttggtatcaa
     4321 gtcggcaccg atggctatgt tccatcgggc aacattcaac ccgtccgaac aatgttaagt
     4381 cagccgtata ctgcaaatat tcccattgaa ggtttattgg cagaagtctg tgtgccctat
     4441 acagatgcct tcgtagaacc cagcaccgaa tcaaaggtcg gctatcgtat ttattatgaa
     4501 acgacccatt gggtcaaagc cttggtggca ggcaaaacag atgggcaacc ctggtatcag
     4561 atccgcgatg ataaatggga tgagttgtac tacgttcgtg ccgatcacat ccgcatcatg
     4621 acgcc
//
LOCUS       DAOQJE010000175         4605 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_11132_length_4605_cov_4.041538, whole genome shotgun sequence.
ACCESSION   DAOQJE010000175 DAOQJE010000000
VERSION     DAOQJE010000175.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 4605)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4605)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4605
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11132_length_4605_cov_4.041538"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(3..998)
                     /locus_tag="PLT08_06020"
     CDS             complement(3..998)
                     /locus_tag="PLT08_06020"
                     /inference="COORDINATES: protein motif:HMM:NF012264.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HNE04052.1"
                     /translation="MKRPELISRILIITGILLAVGAPLFLWARTPLIHARMAEDGGWN
                     PDVIQAETGKPLHLKLTSDDVVHGFAVGQMDMQSVDILPGKVTEITLTFDKPGIYTFF
                     CTRWCGLNHWRMRGTIEVTGGSDIITAGGSSEPASVPLYVSLDLDIDAPHDSPVIPNE
                     KPSAMNGQLLGANLPIDQSLDYYLSHSPYQVFTDLSDTSLTESQKWDVVAYLWQSNTS
                     SKSLANGEQLYAQNCAACHGENGAGDGVFADDLATAGEASMQTMEGAMDMVMQNPVDF
                     TDPRRMLGASPALLQGKILRGGMGTGMPMWGVIFTEEQIWDLIAYIYSFQFEYEK"
     gene            complement(995..2347)
                     /locus_tag="PLT08_06025"
     CDS             complement(995..2347)
                     /locus_tag="PLT08_06025"
                     /inference="COORDINATES: protein motif:HMM:NF024211.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HNE04053.1"
                     /translation="MKEILLPNMNRIELTRIPFIKNTLKSRYPQLAVFIVMLVGYIFA
                     ILAGLIGTPVGSHNFSIVFVWIAWWAILILVAVPFFGRGWCAVCPIPLPGEWLQRGAI
                     LAPPDKRPKWLNLRVPKMFRNIWMQNISFLLLALFSSALLTTPNITGIVLAAMLFVAI
                     GLSAIFERRAFCRYLCPVGGFIGLYSQTAPIELRIKDKQVCVKCVGKPCYNGSDAGYG
                     CPWDVFPGGMTKNTYCGLCMECIRTCPHDNIAVNLRPFSADLAKPTARLDEAFKAFIM
                     FGAAMIYAGVLLGPWGTLKDAAYNVGTGAWFMYAVIFLAIIFIVLPGLFTLGIMTTKG
                     RQSLKQRFMSLSTALIPLGLMFWVAFSLSFVLTNASYILAALSDPLALGWNLFGTANA
                     AWQPMLTTILAPAQTLALVVGLVWSARTAQKVNEAGTSTIPVIVYCFVATSIMLWLLL
                     "
     gene            complement(2325..2804)
                     /locus_tag="PLT08_06030"
     CDS             complement(2325..2804)
                     /locus_tag="PLT08_06030"
                     /inference="COORDINATES: protein motif:HMM:NF024864.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupredoxin domain-containing protein"
                     /protein_id="HNE04054.1"
                     /translation="MRLHFSLLLLVMAGLVVAFAPLPVPAVAPQERTFEVDARQYAYS
                     PSELKVNAGDTVTIKLVSTDVVHGLYVDGYDISVEADPGQSARLTFVADKPGSFRFRC
                     NVTCGAMHPFMIGKITVGTNDWLYRSIGLASLAVIGFFPLSSFLNQSKKKDERNIAS"
     gene            complement(2809..3255)
                     /locus_tag="PLT08_06035"
     CDS             complement(2809..3255)
                     /locus_tag="PLT08_06035"
                     /inference="COORDINATES: protein motif:HMM:NF025170.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PCYCGC motif-containing (lipo)protein"
                     /protein_id="HNE04055.1"
                     /translation="MFSKTRKFLFLLVILALTATAVSACSTSNPDDAHLAMMPLDQMP
                     MEVQSAPVAVQEAYQFNTANPDIMQDIPCYCGCGDIGHTSNYDCYVSNIDDKNNITFD
                     NHALGCSICVDITQDVMRMLRDGKSPQDARVYIDATYSKYGPSNIP"
     gene            complement(3380..3679)
                     /locus_tag="PLT08_06040"
     CDS             complement(3380..3679)
                     /locus_tag="PLT08_06040"
                     /inference="COORDINATES: protein motif:HMM:NF012621.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-associated domain-containing
                     protein"
                     /protein_id="HNE04056.1"
                     /translation="MSDNCHVEPIQKSALDHVIQSANRILLSVSGMGCPNCATRVRNG
                     LLLLDGVHDADVMLNMRMAEVYFDENMVSAEMLIQAVIGAGNDGRHNYQAQVIAS"
     gene            complement(3692..3895)
                     /locus_tag="PLT08_06045"
     CDS             complement(3692..3895)
                     /locus_tag="PLT08_06045"
                     /inference="COORDINATES: protein motif:HMM:NF016811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YHS domain-containing protein"
                     /protein_id="HNE04057.1"
                     /translation="MSFKDPVCGKRVNRGKAHITIEFEGVNYFLCCPQCQAQFERSPK
                     TFAKPELGEKARKVQHYPVKQHN"
     gene            complement(4046..>4605)
                     /locus_tag="PLT08_06050"
     CDS             complement(4046..>4605)
                     /locus_tag="PLT08_06050"
                     /inference="COORDINATES: protein motif:HMM:NF019351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="multicopper oxidase domain-containing protein"
                     /protein_id="HNE04058.1"
                     /translation="TPFDILRLMIGEKGADVNPLPSQLSTIERHAVNDAVNGNNPRSF
                     RLAMQGMVHTINNRLFEMDAVAQDEIVRLNDLEVWEFVNLEGSGGGMGMGMMNMEMPH
                     PMHIHGVQFQVLERQIARGFESAYQELSGGFVDEGWKDTVLVMPGETVRVLVRFENYT
                     GIYLYHCHNLEHEDAGMMRNYRIKG"
BASE COUNT         1135 a         1130 c         1274 g         1066 t
ORIGIN      
        1 ccttactttt catattcaaa ctggaacgaa tagatataag cgatcaagtc ccagatttgt
       61 tcttcggtaa aaatgacgcc ccacatgggc atgcctgtgc ccatgccgcc gcgcaggatt
      121 tttccttgta ataatgcagg gcttgcacca agcattcgcc gcggatcggt gaaatctaca
      181 gggttttgca tcaccatgtc cattgcacct tccattgttt gcatggaggc ttcgcctgct
      241 gtagccagat cgtctgcaaa gactccgtca cctgcaccgt tctctccgtg acacgccgcg
      301 cagttttggg cataaagttg ctctccattg gcaagagatt tggatgaggt gttggattgc
      361 caaagatagg cgacaacatc ccacttttgg gattcggtta aggatgtgtc cgataagtcg
      421 gtgaagacct ggtaggggga gtgagaaaga tagtaatcga gtgattggtc gattggtaaa
      481 ttagctccaa gtaattgacc attcattgct gatggctttt catttggaat aacaggcgag
      541 tcgtgcggag cgtcaatgtc caggtcgaga gaaacgtaga ggggaacaga agcaggctcg
      601 gatgagcccc ctgcggtgat gatatccgaa ccgcctgtta cttctatcgt gccgcgcatg
      661 cgccagtgat tgagtccaca ccaacgggtg cagaagaagg tatagatccc aggcttgtcg
      721 aaggtcaacg tgatctcggt gactttgccg gggaggatgt ccacactttg catgtccatc
      781 tgaccgactg caaagccatg cacaacatca tcggaggtga gtttcaaatg aaggggcttg
      841 cccgtttctg cttgaatcac atcggggttc cagccgccat cctccgccat gcgggcgtgg
      901 atcaaaggcg tgcgcgccca aagaaacaat ggagcaccaa cggcaaggag tatccctgtg
      961 ataatcagga tgcgtgagat gagttcggga cgtttcatag caataaccat agcatgatgg
     1021 atgtggcgac gaagcagtag acgatgacag ggattgtgga cgtccccgct tcattcacct
     1081 tttgggctgt tcgcgccgac cagaccaatc ctacgaccag agcgagtgtc tgcgcgggcg
     1141 caaggatcgt ggtgagcatg ggctgccatg cggcatttgc ggtgccaaac aaattccagc
     1201 caagcgccag cggatcggac aatgcggcga gaatgtaaga agcatttgtt agaacgaagg
     1261 atagactgaa agcaacccag aacatgagtc ccagtgggat gagagcagtg gaaagagaca
     1321 tgaagcgttg tttgagagat tgcctgcctt tggttgtcat aatccccaaa gtgaaaagcc
     1381 caggcaggac gataaagatg atcgcaagaa aaattactgc atacatgaac catgcgcctg
     1441 taccgacgtt gtatgccgcg tctttgagtg tgccccacgg accaagcaac acgcccgcgt
     1501 agatcatggc ggcgccaaac ataatgaagg ctttgaaggc ttcgtcgagt cgcgctgtcg
     1561 gtttggcaag gtcagcagag aaagggcgta ggttgaccgc gatattgtcg tgcgggcagg
     1621 tgcggataca ttccatacaa agtccgcaat aggtgttctt ggtcatgccg ccggggaaga
     1681 catcccacgg acagccgtag cccgcgtccg agccgttgta gcagggcttg cccacacact
     1741 tcacgcaaac ttgtttatct ttgatgcgta gttcgatggg cgcggtttgt gaatacaagc
     1801 cgatgaatcc acccacggga cagagataac ggcagaaggc gcggcgctcg aagatggcgc
     1861 tcagtccgat tgcgacaaag agcatggcag ccaggacaat gccagtgatg tttggggtag
     1921 tgagcagggc actgctaaag agagccagaa ggagaaacga aatgttctgc atccagatat
     1981 tgcggaacat ttttggcaca cggagattca accatttcgg tcgtttatct ggcggggcaa
     2041 ggatcgcgcc gcgttgcagc cattcccctg gaagcgggat cggacagacc gcgcaccagc
     2101 cgcgtccgaa gaaggggacc gcgacgagaa tgaggatcgc ccaccacgcg atccaaacaa
     2161 agacaatgct gaagttgtga ctgccgacag gcgttccgat gagtcccgca aggatggcaa
     2221 agatgtaacc gaccagcatg acgataaaga ctgccaattg cgggtaacgg cttttgagag
     2281 tgttctttat aaaagggata cgggtaagtt cgatgcggtt catgttagga agcaatattt
     2341 ctttcatctt tcttcttact ttgattcagg aaagacgaaa gaggaaagaa gccaatgact
     2401 gcaagagacg ccaaccctat tgagcgataa agccaatcgt ttgtgccaac ggttattttc
     2461 ccgatcatga agggatgcat ggctccgcaa gtgacgttac agcggaaacg gaaggaaccg
     2521 ggtttgtcgg caacgaaggt taacctggcg ctttgtccgg ggtcagcttc gacggaaatg
     2581 tcgtaaccat ccacatatag cccatgaaca acatctgtgc tgaccagttt gatggtcacg
     2641 gtgtcgccag cgttgacctt cagttcggag ggggaatagg catattggcg cgcgtccacc
     2701 tcaaaggtcc gctcctgagg cgcaacggct ggaactggca gaggcgcgaa cgcgaccacc
     2761 agtccagcca tcacgagaag gagaagagag aaatgaaggc gcatgagatt aggggatgtt
     2821 ggagggtccg tacttggaat acgttgcgtc gatgtaaacc ctggcatcct gcggactttt
     2881 gccgtcccgc aacatgcgca tcacatcctg ggtgatgtcc acgcagatgg aacagccgag
     2941 ggcatggttg tcgaaggtga tgttgttttt gtcatcgatg ttcgagacgt agcaatcgta
     3001 attggaagtg tgaccgatgt cgccgcatcc gcaatagcag ggaatgtcct gcatgatgtc
     3061 tgggttggcg gtattgaact ggtaggcttc ctgcactgcc acaggcgcgg actgaacttc
     3121 cattggcatt tggtcgagcg gcatcatggc aagatgcgcg tcatcagggt tggaagtgga
     3181 gcaggcagaa accgcagtag cggtcagcgc caaaataacg agaaggaaaa ggaactttcg
     3241 ggtctttgaa aacatattgg gtcctccgtg ttcggatgac ccaatagtag tctgaccatt
     3301 aagttatctc cagcgcagta cggcgggtgg ggatatacgc caaatggctt atgatgaatg
     3361 tcatctgacg aaaggtcatt tacgatgcta tcacttgcgc ttgataattg tgacgtccat
     3421 cgttccctgc tccaattacc gcctggatca gcatctcggc agagaccata ttctcatcga
     3481 agtaaacttc cgccatgcgc atgttcaaca tcacatcagc gtcatggacg ccatccagta
     3541 acagaagtcc attacgtacc cttgtggcac agttgggaca gcccatccct gaaacggaca
     3601 gcaggattcg gttggcagac tggatgacat gatccagcgc ggacttttgg atcggttcaa
     3661 cgtgacaatt gtcgctcata atggtttcct tttaattgtg ttgttttacg ggatagtgct
     3721 ggactttcct tgccttctct cccaattcgg gcttggcaaa ggtttttggg gaacgttcaa
     3781 actgagcctg gcattgcgga cagcacagaa aatagttcac accttcgaac tcgatggtga
     3841 tatgggcttt gccgcgattg acgcgttttc cacagacggg atctttgaat gacatatcga
     3901 aactccttgg ttgtgatctg acggttggat tgtagagtcg ctactggaat tattgaagcg
     3961 cagaatggct tgatgtagga ataagcaatt ctgcgtatcg cccttctaaa aaaaacaggc
     4021 tccgattttt ggagcctgtt cctactcaac ctttgatcct gtagtttcgc atcatgcccg
     4081 catcctcatg ttcgagattg tggcagtgat aaagatatat ccctgtgtaa ttctcgaagc
     4141 gcacaagcac ccgaacggtt tcgcctggca tcaccagcac ggtatccttc caaccctcgt
     4201 ccacaaagcc gccgctcaat tcttgatacg cggattcaaa tccgcgcgcg atctgcctct
     4261 caagcacttg gaattgcacg ccgtggatgt gcatgggatg cggcatttcc atattcatca
     4321 tgcccattcc catgccaccc ccgctgcctt cgagattgac aaactcccaa acttccaggt
     4381 cgttcagccg cacgatctca tcctgcgcca ctgcgtccat ctcgaacagg cggttgttga
     4441 tggtgtgaac catgccctgc attgccagcc tgaacgagcg cggattgttt ccattgactg
     4501 catcgttcac agcatgtcgt tcaatcgtgg agagttgcga gggcaacggg ttcacgtccg
     4561 ctcctttctc gccgatcatc aaacgcagga tgtcgaacgg cgtac
//
LOCUS       DAOQJE010000176         4582 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_11220_length_4582_cov_3.882925, whole genome shotgun sequence.
ACCESSION   DAOQJE010000176 DAOQJE010000000
VERSION     DAOQJE010000176.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 4582)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4582)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4582
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11220_length_4582_cov_3.882925"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1033
                     /locus_tag="PLT08_06055"
     CDS             <1..1033
                     /locus_tag="PLT08_06055"
                     /inference="COORDINATES: protein motif:HMM:NF037015.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HNE04059.1"
                     /translation="TPVSISLSSNDEGSGVWKVEHRINGGVWKDGSVFQVTEDGPYSI
                     DYRVTDRAGNVFTLPDGMVKDAAAPGVTILTPSKQTIVDKNVIISGSSDDATSGLDAV
                     EISIDNGKTWFPLNNGNWSFDFDSSDMKNGELKILSRAFDKAGNQAQSESTILLANHG
                     PQVSIPDQWIYTEAGGLSIEPQYFEVASVTVTITNDAGEVIRVTKSSENPGYIAWDGK
                     QNGELLPAGDYPVVVVVCDTNNKCSTTAGRIEIPAFNYIIPTPEQEGKPEEIIVVPPP
                     PIVVEEQTPIIKFTSAVFGSTRKAGISVAVLASGFLLLFAIQNVSDPRPRAMRSLAGT
                     IRKNMIPKE"
     gene            1043..1618
                     /locus_tag="PLT08_06060"
     CDS             1043..1618
                     /locus_tag="PLT08_06060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE04060.1"
                     /translation="MSTEMLMFIMLSVFVVILTILSYSQASSMREMERVVQDYYAMQL
                     RTARRAYAGALDTLNPYEWVSNRLSARQSKPVQIIEVIRSIPEIGAVDLRTNDNRRVV
                     VSVHPKLDILLFDRRAKVSKRSKKRAADRIADFAVRPVLDGSRYGWGVTTIEVAMSKT
                     DEFFDMEADAVGKKLNVDWKTPTRLYFHVVD"
     gene            1605..2066
                     /locus_tag="PLT08_06065"
     CDS             1605..2066
                     /locus_tag="PLT08_06065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE04061.1"
                     /translation="MLWTNTAFELAVLIPILITGWQDYRTREASNLITIPYFFIGVGM
                     GIYRYHEDHSMWYVFLVQGLITLITWFNGMGGADWKMQCGLFGLHPLMGLVSVLAEGI
                     WGAVLLLRHGTQARFPKLSVMALSSALTFLIDISIMPNIVQFLPGRIVPTA"
     gene            2153..2350
                     /locus_tag="PLT08_06070"
     CDS             2153..2350
                     /locus_tag="PLT08_06070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE04062.1"
                     /translation="MTNILTYTFTWIRANRFTSMLFDKRADEFGEKAVVIVLVVLGGL
                     AAFAAFGSAVSGRITEAAGAI"
     gene            2357..2776
                     /locus_tag="PLT08_06075"
     CDS             2357..2776
                     /locus_tag="PLT08_06075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE04063.1"
                     /translation="MIAVLRRLLCSKRADEFAEAAIALPVVVFITIAFINLTMAGFAS
                     VNANNAANYAARVGSVNQQSPGPAAYSAALESINHAKIGDYQVGVSGGGFPGAQINVV
                     VTWNVPNFMGGIAAFFGASFGDDFTGTATSVFRQEGW"
     gene            2777..3205
                     /locus_tag="PLT08_06080"
     CDS             2777..3205
                     /locus_tag="PLT08_06080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE04064.1"
                     /translation="MLQNKRGDIYAWVAALMLFIAVPLASLSIDIVRMMYVRGHLHTA
                     TDAACQAAVNTLDVSHFTTTGEARVDRGRGRTNAVNVFRANLVDADTVRYTESLVVDF
                     PGPRLAHCTSTAQVDHLIPMTPTMNVVVETTSEMRGSYGN"
     gene            3195..3638
                     /locus_tag="PLT08_06085"
     CDS             3195..3638
                     /locus_tag="PLT08_06085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE04065.1"
                     /translation="MEIDLEFGDENNWVGITAIVLLVMIALVGLASIGERMTPVNELG
                     QPRVMNADDWRVHEATRAYNEEISVLRDDIREVSLLLQKSPNPIAAQILYERVAGHAN
                     NGQSTTQIARQLTVTAAESVLAWSNGLVDRASAMNAVNEAIEVLK"
     gene            3644..>4582
                     /locus_tag="PLT08_06090"
     CDS             3644..>4582
                     /locus_tag="PLT08_06090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE04066.1"
                     /translation="MPDIAFHGQRSLSDLLEAERSMRAASDETVRGLRDVLLSVANRL
                     DGKRMEALRHVDPKIPGNWSAKQWKEFVDAIPAPDAWGAMTTAAISMSNNEENSRYVE
                     ELKAEIQNLMVDLENANTRIADMEKQQEAILLKTVRKNPVELKPMPSTRPNGYVFPPL
                     DALVHEAESRLLLFPNPPGKLMNGLVSVGSRDGKNRLIFLQRVFVSLFALGARGISNT
                     LELNHLVALPGEAQPNSGSVGKVLNDIEKNGLIAIERFEMSEPKSTLKVAKLTEKGEA
                     LFKGLFPKEKLIESEVSTSMQWAHNDRSRATALMYFA"
BASE COUNT         1169 a         1079 c         1204 g         1130 t
ORIGIN      
        1 tacccccgtc agcatatctc tcagcagcaa cgatgagggg tcaggcgtat ggaaagtcga
       61 acaccgcatc aacggtggag tttggaaaga tggatctgta tttcaggtga cagaggatgg
      121 tccgtacagt attgactatc gcgttaccga tcgtgcaggt aatgttttta cattgcctga
      181 cggcatggta aaagatgccg ctgcgccagg tgttaccatc ctgacgcctt caaagcaaac
      241 cattgtagat aagaacgtga tcatatcagg cagttcagat gatgcaacgt ctggcttgga
      301 tgctgtcgaa attagtattg acaacggaaa gacatggttt ccattgaata atggcaactg
      361 gtcattcgac ttcgactcgt ccgatatgaa aaatggcgaa ctgaagatac tctcacgcgc
      421 cttcgacaag gccggaaatc aagcgcaatc tgaatccaca atattacttg caaaccatgg
      481 accgcaggta agcatacccg accaatggat atacaccgag gcaggagggt tatccatcga
      541 gcctcagtat ttcgaggtcg cttctgtgac ggtcacgatc acgaatgatg cgggcgaagt
      601 gatccgtgtg acaaaatcat cagaaaaccc tgggtacatt gcctgggatg gaaaacagaa
      661 tggagagctg ctgcctgccg gtgattatcc ggttgtcgta gttgtttgcg acacgaacaa
      721 taagtgctcc accaccgcag gacggatcga gatccctgct ttcaactaca tcattccaac
      781 cccggagcag gaaggtaaac cggaagaaat aatagtagta ccgcctccgc cgattgtcgt
      841 ggaagaacag acgccgatca tcaagttcac atcggctgtt tttggcagca cacgcaaggc
      901 aggcatcagt gtcgctgtcc tggcaagtgg ttttctattg ctatttgcca tacaaaatgt
      961 atctgatccc agacccaggg caatgagatc cctggctggc acgatccgaa aaaacatgat
     1021 ccccaaggag tagtaacaaa ccatgtccac agaaatgttg atgttcatta tgctttcggt
     1081 ctttgtcgtg atactcacga tcctgagcta cagccaggcg tcctcgatgc gggaaatgga
     1141 acgtgtcgta caggattatt acgccatgca attacgcaca gcccgtcgcg cttacgcggg
     1201 ggcgctggat acgctcaacc catacgagtg ggtatccaac cgactctctg cccggcaatc
     1261 caagccggtg caaataatcg aagtgatccg ctcgatcccc gagatcggag ctgtggatct
     1321 gcgaactaac gataatcgca gggttgtcgt cagtgtgcat cccaaattgg atatcctgct
     1381 ttttgatcga cgtgcaaagg tgtcaaaacg atcaaaaaag agagctgcgg atcgaattgc
     1441 cgattttgct gtgcgtcctg ttttggatgg cagccgatac ggatggggag tgaccaccat
     1501 tgaagtcgca atgtcaaaga cggatgaatt ctttgatatg gaagcggatg ctgttggaaa
     1561 aaagctcaat gtagattgga aaactcctac gaggctgtac ttccatgttg tggactaaca
     1621 cggcattcga gctggcagtt ctgatcccaa tcctgatcac aggctggcag gactaccgca
     1681 cccgtgaagc gtcaaacctc atcaccatcc cgtatttctt tattggggtg gggatgggga
     1741 tataccgtta ccacgaagac cactcgatgt ggtacgtttt tcttgttcaa ggattgatca
     1801 cactgattac atggttcaat ggaatgggtg gcgcagactg gaagatgcag tgcgggttat
     1861 tcggattgca cccattgatg ggtttagtat cggtattggc agaaggcata tggggagcag
     1921 tcctgctttt gcgtcatggt acgcaggcga ggtttccaaa attgtcggtt atggctttat
     1981 cctcagcctt gacatttctt attgatattt ctataatgcc gaatattgtg caattcctcc
     2041 ccgggaggat tgtgccaacc gcatgaaaga cgcggcaggc acataccttg ggagaattgc
     2101 atttttttat ttaattcccc ccaataaatt cactattcaa aggattaacg aaatgacaaa
     2161 catcttgacc tatacattta catggatccg tgcaaaccgc tttacttcaa tgctttttga
     2221 caaacgcgcg gatgagtttg gcgaaaaagc cgttgttatt gtattggtgg ttctcggcgg
     2281 attagctgcc ttcgcagcgt ttggttctgc tgtgtcagga cggatcaccg aagccgctgg
     2341 agccatttaa cctgccatga tcgcagttct tcgcaggttg ttgtgctcaa aacgcgcgga
     2401 tgaattcgca gaagcggcaa ttgcgcttcc agtagtcgta ttcattacga tcgcatttat
     2461 caacctgacc atggcagggt ttgcttccgt aaatgcaaat aacgctgcga attatgccgc
     2521 ccgtgttggc tccgttaatc aacagagtcc gggtccggct gcatattcgg cggcattgga
     2581 gtccatcaat catgccaaga ttggcgatta ccaggtgggc gtttccggcg gtggtttccc
     2641 tggggcgcaa atcaacgttg tagtgacttg gaatgtccct aatttcatgg gagggatcgc
     2701 agctttcttt ggagcgagct tcggcgacga cttcacagga acggcaacca gcgtgttccg
     2761 tcaggagggc tggtaaatgc tgcaaaacaa gcgcggtgac atatatgcgt gggtggcagc
     2821 gttgatgctg tttattgcag tgccgctggc aagtctgagc atagacattg ttcgtatgat
     2881 gtatgttcgt ggacacctgc acactgccac agacgcggcc tgccaggcgg cagtcaatac
     2941 cctggacgta tcacacttca ccaccacagg cgaagctcgt gttgaccgtg gtagagggcg
     3001 cacaaatgca gtaaatgtgt ttcgtgcaaa ccttgtggat gctgacaccg tgcgatacac
     3061 cgaatcgctt gttgtggact ttcctggtcc gcgcctggct cattgcacat cgaccgcgca
     3121 ggtcgatcac ctcattccca tgactcccac catgaatgtc gttgtggaaa ccacatcaga
     3181 gatgcgaggt agttatggaa attgatctcg aattcggcga tgagaacaat tgggttggta
     3241 tcacagctat tgttctactt gtcatgatcg cccttgtagg acttgcctcc atcggtgaac
     3301 gcatgacgcc cgttaacgaa ttggggcagc cgcgagtgat gaacgcggat gactggcgcg
     3361 tccatgaagc aacccgcgcc tataacgagg aaatctcagt cttgcgtgat gatatccgtg
     3421 aagtgtccct gctgttacag aaatcgccaa atccaatcgc ggcacaaatt ctttacgaac
     3481 gggttgctgg tcacgcaaat aacggacagt ccaccacgca gatcgctcgt cagcttactg
     3541 tgactgccgc cgagagcgta cttgcatggt cgaatggatt ggtggatcga gcgtcagcca
     3601 tgaatgcggt gaacgaagcc attgaggtat tgaaataatg acgatgcctg atattgcctt
     3661 tcatgggcaa cggtctttat ctgatttact tgaagcagaa cgtagtatgc gtgctgccag
     3721 cgatgaaaca gtgcgcggtt tacgagacgt gctgctaagt gttgccaatc gtctggatgg
     3781 taaacgcatg gaagcgttga ggcatgttga ccccaagata ccaggaaatt ggtcagctaa
     3841 acaatggaaa gagtttgtcg atgcaattcc tgcccctgat gcctggggtg caatgacaac
     3901 cgcggcaatt tccatgagta ataatgagga aaacagcaga tacgtggaag agttaaaagc
     3961 ggaaatccag aatctgatgg tcgatctgga aaacgctaat acccggatcg ccgatatgga
     4021 aaagcagcag gaagcaatat tgctaaaaac cgtcaggaaa aatccggttg aattgaaacc
     4081 aatgccttcc accagaccca acggatatgt tttccctccg ttggacgccc tggtccatga
     4141 agcagaatcc aggcttttat tattcccaaa ccctcccggc aagttgatga acgggctggt
     4201 aagcgtcggg tcgcgtgacg gcaagaatcg ccttatcttt cttcagcgtg tgtttgtgtc
     4261 tctattcgcc ctgggcgccc gtggcatatc caatactctg gaattgaatc atttggtcgc
     4321 attgcctggc gaggcgcagc ccaattcggg ttccgtaggg aaggtattga acgatattga
     4381 gaagaatgga ctgatcgcga tcgagcgatt tgagatgtcc gagccaaagt ctacattgaa
     4441 ggttgcaaaa ctgaccgaaa aaggcgaggc gttattcaaa ggtctgttcc caaaggaaaa
     4501 acttattgag tctgaagtat ccacctccat gcagtgggca cataacgatc gctccagggc
     4561 aactgccctc atgtattttg ca
//
LOCUS       DAOQJE010000177         4522 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_11444_length_4522_cov_5.095366, whole genome shotgun sequence.
ACCESSION   DAOQJE010000177 DAOQJE010000000
VERSION     DAOQJE010000177.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 4522)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4522)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4522
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11444_length_4522_cov_5.095366"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            56..499
                     /locus_tag="PLT08_06095"
     CDS             56..499
                     /locus_tag="PLT08_06095"
                     /inference="COORDINATES: protein motif:HMM:NF040341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6174 domain-containing protein"
                     /protein_id="HNE04067.1"
                     /translation="MKKILLLVLALLLVACSAASEYDQNIKKWQDADVAHYRFDLFIG
                     CFCPFRNVMPLTIEVQNGKVVSMTQADGMVVATDDPSYELFTSVASMDAIFATLDSSL
                     NGDADEVLVTYDTMYGFPTSISIDHIKEAVDDEIFYQVENFEVLP"
     gene            complement(532..1215)
                     /locus_tag="PLT08_06100"
     CDS             complement(532..1215)
                     /locus_tag="PLT08_06100"
                     /inference="COORDINATES: protein motif:HMM:NF012257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HNE04068.1"
                     /translation="MKTIESTLKQKMDALRGNQYFDDVSDEILKDIATHTRLGEFQRG
                     DVLFWEGDPCEGLFILQQGSAKIFRLSPQGRQYIVRILQEGDTFAEVPAFDEGTSPVN
                     VEALETCRTWIIDKNKLHTLVMEHPAFAQKVLVNFGRMLRGMVRMVSEMAFYQVTHRL
                     ARLIDTELPQDKSAAWTQEQLAARLGTVREVVARSIKELERSGAIKIEDRRIQIADRE
                     IFEQWIMPS"
     gene            complement(1221..1736)
                     /locus_tag="PLT08_06105"
     CDS             complement(1221..1736)
                     /locus_tag="PLT08_06105"
                     /inference="COORDINATES: protein motif:HMM:NF012383.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyltransferase family protein"
                     /protein_id="HNE04069.1"
                     /translation="MKPEPRRQEAITWEEVDKLIDHLIPQFRREFTAMVMITRGGIIL
                     GGMLAEAMDITHILTAAVDFPAQTQSQQSSAFMAWPEFIQFPADDKLRGRPTLIVDDV
                     WGSGRTITAVKNRVSAAGGFPETCVLHFNPYRNLFGSVRPDYYAAVTDAFITYPWEIN
                     RGPDQVLLGEV"
     gene            complement(1799..2701)
                     /locus_tag="PLT08_06110"
     CDS             complement(1799..2701)
                     /locus_tag="PLT08_06110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE04070.1"
                     /translation="MQSFSRGWSFLKQAWSMAFKDKDLLKPSIYALIVGAIVSVIGII
                     PIAIAAFAFGNSDIGNIIIYAMGALLVFVQFIVTYMFSAMTVYLIYGYLSEGDGRMDK
                     AWEIVRRDFFDILTLAAASTVVNLIKNAAQRNRRGGVGASIARSATGLLETLWTEAAA
                     LVLPAMVIDDLNLKDGMQRIWKITKENFLLVGISTIGVRFVTGLIGFVFSMIGLILAF
                     AIGGGLAFASGGDTTLSIIGVVVGALIFFAFVMISSVFSSYTTTAYHTCLYIWAREVE
                     KATAQGMAPAQVRAPAPLAAVLAS"
     gene            2825..3205
                     /locus_tag="PLT08_06115"
     CDS             2825..3205
                     /locus_tag="PLT08_06115"
                     /inference="COORDINATES: protein motif:HMM:NF012264.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HNE04071.1"
                     /translation="MKHKFPFLVLVAALFLSACATAATPVPTPTLDPIAKQGQAVFNA
                     RCATCHALVPDTIIIGPSLYGVATRAETRVEGLSAEEYITMSILRPGDYVVEGFNNVM
                     ITNLAKELTSEELNAIVAFLMTMK"
     gene            3253..>4522
                     /locus_tag="PLT08_06120"
     CDS             3253..>4522
                     /locus_tag="PLT08_06120"
                     /inference="COORDINATES: protein motif:HMM:NF015131.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c peroxidase"
                     /protein_id="HNE04072.1"
                     /translation="MKVWIKRIFLGLGVLLLVGAALAAFIPLPPAVELPEEKWGAGSS
                     SVEPAWSGLQRDFPASNETNENPTTPEKVELGRLLFFDPVLSQNNDMSCASCHHPDMG
                     FTDGNAQAIGADGEALPRSAMSLWNVAYNTTFFWDGRAASLEKQMETPLTSVDEMGSS
                     PDDIVAEIKAIPEYVDLFEKAFGSSDAVTFENVTYAIAAFERSLVTNNSPFDRYAAGD
                     VNALTPSQRRGLGLFRSAATRCFECHAAPTFADESFSVTGVPDLPGQPHDEGRKAIEP
                     SGLDGAFKAPTLRNIALTAPYMHNGVFNTLEEVVDFYAKGGGRDAGVADVDIHVLGFE
                     LTEQEKADLVNFLYALTDESNLPEIPTSVPSGLDVVKKLEPVQARQVVSEINAAESAA
                     GSTSTHEPVTLRVEPGQTIQEVVDQALPGDT"
BASE COUNT         1151 a         1135 c         1093 g         1143 t
ORIGIN      
        1 ttttatttgg gaacatttct gggataatct tcgttctcta aacaaggagt tttacatgaa
       61 gaaaatttta ttgctcgttc ttgcgctttt actggttgcc tgttctgccg cttctgagta
      121 tgaccagaat ataaagaaat ggcaggatgc agatgttgcc cattatcgtt ttgacctgtt
      181 catcggctgt ttctgtccgt ttcggaatgt gatgccattg acaatcgaag ttcaaaatgg
      241 gaaagtggtt tcgatgaccc aggcagatgg tatggttgtt gcaaccgatg acccctcgta
      301 tgaattgttc acgtccgttg ccagcatgga tgctattttt gctacattgg attccagctt
      361 gaatggcgat gcggacgaag tgctcgtcac gtatgatacg atgtatggtt tccccacctc
      421 gatttccatt gaccatataa aagaagcggt ggatgatgag atcttttatc aggtggagaa
      481 ctttgaagtt cttccataaa ataaaaagcg gcgaattcgc cgctttttat tttaacttgg
      541 catgatccac tgctcaaaga tttctctatc tgcgatctga atgcgacggt cttcaatctt
      601 gatcgcgccg ctgcgttcca attccttgat cgatctcgcc acgacttcac gcacggttcc
      661 caaccgcgcc gcgagttgtt cctgcgtcca ggcagcggat ttatcctgtg gcagttcagt
      721 gtcgatcaag cgcgccagcc gatgggtgac ttgatagaac gccatctcgc tgaccatgcg
      781 caccatgccg cgcagcatcc taccaaagtt gaccaagacc ttttgggcaa aagcaggatg
      841 ttccatgacc agtgtatgta gtttgttctt atcgatgatc caagtgcggc aagtttccag
      901 cgcctctaca ttgaccgggc tggtgccttc atcgaaggca ggaacttctg cgaaggtgtc
      961 gccctcttga agaatgcgga cgatgtattg ccgtccctgt ggcgagaggc gaaatatctt
     1021 ggcgctgccc tgttgtagga tgaacaaacc ttcgcaggga tcaccttccc aaaaaagaac
     1081 atccccgcgc tggaattctc caaggcgggt atgggttgct atgtctttca agatttcatc
     1141 tgatacatcg tcaaaatact gatttccccg caaggcgtcc atcttttgtt ttaaggtgga
     1201 ttcgatcgtt ttcattccaa ttacacttcg cccaatagca cctggtctgg tccgcgattg
     1261 atttcccaag gataggtgat gaaagcatct gtcacagcag cgtaataatc ggggcgcaca
     1321 ctgccaaaca aattgcggta tgggttgaaa tgcaacacac aagtttcagg gaacccgcct
     1381 gccgctgaaa cgcgattctt tacagccgtg atcgtacgcc cagaacccca cacatcatcc
     1441 acgatcaagg ttgggcgacc acgtaattta tcatccgcag gaaactgaat aaactcgggc
     1501 cacgccatga atgcagaaga ttgctggctc tgagtctggg cagggaaatc caccgcagca
     1561 gtcaggatgt gggtaatatc catggcttct gctaacatgc cgccaaggat aatcccgccg
     1621 cgcgtgatca tcaccatggc ggtaaattca cggcgaaatt gtggaatgag gtggtcaatc
     1681 agcttgtcca cttcttccca agtgatggct tcttgtcggc ggggttcagg tttcatagta
     1741 gatattttat caaaagaccc taaaggatgc tgacctttag ggtctcatca tatatttctt
     1801 aggatgcaag aaccgccgcg agcggagccg gagcccgtac ctgtgccgga gccattcctt
     1861 gcgccgtggc tttttctact tcacgtgccc agatataaag gcaggtgtga tatgccgtcg
     1921 tggtatacga gctgaataca cttgaaatca tcacaaaggc aaagaagatc aacgccccaa
     1981 ccacaacacc tataatggaa agagtggtat ctccaccaga agcgaaggca agtccgccac
     2041 cgatggcaaa agcgagaatc aagccgatca tgctgaaaac aaacccaatc agtcctgtaa
     2101 cgaagcgcac accaatggtg ctaataccaa ccaataggaa gttctctttg gtgattttcc
     2161 aaatacgttg cattccatct ttgagattga gatcatcgat caccatggca ggcaaaacga
     2221 gagcggcggc ttccgtccac aaggtttcca acaaacccgt tgcagaccgg gcaatgctgg
     2281 cgccaacccc accgcgacga ttgcgttgag ccgcgttttt gatcaagttc acaactgtag
     2341 aagcagcagc caaggtaagg atatcgaaga agtcacggcg gacaatttcc cacgctttgt
     2401 ccatgcgacc atcgccttcg gaaagatacc cataaataag atacaccgtc atggctgaga
     2461 acatataagt cacaataaat tgaacgaaga ccaataaggc acccatggca taaatgatga
     2521 tgttcccgat atcgctgttc ccaaatgcaa aagcagcaat cgcaatagga ataattccga
     2581 tgacagaaac aatagcaccg acgatcaaag catagataga cggtttgaga agatccttat
     2641 ctttgaatgc catgctccag gcttgcttta aaaatgacca accacgtgaa aaactttgca
     2701 tgctacactc tcctataaac gaaccgaagt ttggacaaac aaattatagc aaacttacca
     2761 aactgcttgc ttccttttta gggtgtccat gtataatcgg gcaattccaa cctcaggata
     2821 tgccatgaaa cataagttcc catttttggt gctggttgca gccctattct tatcggcttg
     2881 cgccactgct gctacacctg tgcctacccc cacacttgac cccattgcca agcaaggaca
     2941 ggcagtcttc aatgcccgtt gtgccacctg ccacgcgctt gtgcccgata caatcatcat
     3001 cggaccatcc ctgtatgggg tcgccactcg cgccgaaacc cgtgtagaag ggttaagtgc
     3061 tgaagaatac attactatgt ccattttacg ccccggtgat tacgtcgtgg agggcttcaa
     3121 taatgtaatg atcaccaacc ttgccaaaga actcacgagc gaagaactga atgcgattgt
     3181 ggcattctta atgacaatga aataacctgc ggtttgtgcg tgcaggaaaa aactgaatac
     3241 agaggagagt gtatgaaagt ttggatcaaa cgtatctttt tagggctggg cgttttgctg
     3301 ttggttggcg ctgcccttgc cgcatttata ccccttccac ccgctgtgga attgcccgaa
     3361 gaaaaatggg gggcaggctc tagcagtgtt gaacccgcct ggagcggatt acagcgtgac
     3421 ttccccgcct cgaacgaaac aaatgagaat ccaactaccc ctgaaaaagt ggaattgggt
     3481 cgcctccttt tctttgaccc ggtcttgtct cagaacaatg acatgtcctg cgcctcatgc
     3541 catcacccag atatgggctt taccgacggc aatgcccaag ccatcggtgc agacggtgag
     3601 gcacttccac gcagcgccat gagcctttgg aatgtggcgt ataacaccac tttcttttgg
     3661 gatggtcgtg ccgcttcatt ggaaaagcag atggaaactc cgctcaccag tgtggatgaa
     3721 atgggcagtt ctcctgatga catcgtagct gaaataaaag cgattcccga atatgttgat
     3781 cttttcgaaa aggcatttgg atcttctgat gccgtgacct ttgaaaatgt cacctatgcc
     3841 attgctgctt ttgagcgttc attagtgaca aataactcgc catttgatcg atatgccgct
     3901 ggcgatgtga atgcccttac tccatcgcag cgtcgcggac ttggactctt ccgctcggcg
     3961 gcgacgcgct gttttgaatg tcatgccgcg ccaacctttg ccgatgagtc attctctgtc
     4021 acaggcgtgc ccgatttgcc aggtcagccg catgacgagg gacgaaaagc cattgaaccg
     4081 tctggtttgg atggtgcctt caaagctcct acgcttcgta atattgctct cactgccccc
     4141 tacatgcaca atggtgtatt caacaccctt gaagaagtgg tggattttta cgccaaaggt
     4201 ggcggacgtg atgctggagt tgcggatgtg gatattcacg tcctcggctt tgagttgacc
     4261 gagcaggaga aagccgacct agtcaacttc ctttatgcgt tgacagacga aagcaatttg
     4321 ccggaaatcc caacctctgt gccttcaggt ttggatgtgg tgaaaaagct cgagcctgtg
     4381 caggcgcgtc aagttgtcag tgagatcaat gctgctgaaa gtgcggcagg ctccacgtct
     4441 acgcatgagc cggtgacctt gagagtcgag ccgggtcaga ccattcagga agttgtcgat
     4501 caggcattgc ccggtgacac ca
//
LOCUS       DAOQJE010000178         4504 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_11526_length_4504_cov_3.473814, whole genome shotgun sequence.
ACCESSION   DAOQJE010000178 DAOQJE010000000
VERSION     DAOQJE010000178.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 4504)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4504)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4504
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11526_length_4504_cov_3.473814"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            12..1421
                     /locus_tag="PLT08_06125"
     CDS             12..1421
                     /locus_tag="PLT08_06125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE04073.1"
                     /translation="MSEMKIAIPLHGKMAARTFHNPSFRQHLVSNGFMPLYFLSPIYY
                     RSFNLNPEQYFELQTDFYDKEYQRHFLLQQLRMLRRFVVITETTDLRFRQMIESKLFD
                     SSLLGMSFQMMFVNFVRKIPGMGQLLSAMEQNLYVSHAHEDHFVQQNIGCVLTPGMGN
                     YAYWNEGKFATEAQRMNIPVFAAITNYDNVVNMGYRMFTPRRLAVWSRQMADEIIKLH
                     RYPASKLEVTGPAHYDRFMQPLSMSRIGFLEKIGLDPSKKTVFFAGGGNISHYFEIYR
                     LFVEEKHRILRGDFNFVVRPHHHGHLRTNPAWNLLEKLFLDAGVYVSNPGSVDATGDR
                     TNELRLDLALDEDYDELHYLLKYSDVLVNIFSTMGLEAAICDLPTIHIGYDAYTIGMK
                     FGLTSAFQQRMTHNRRPLRLKASKVAKNEMDLIKYIEEYLGDRSMDSEVRREYAVSEC
                     GELDGLSSSRLVEMIKSHI"
     gene            1706..2638
                     /locus_tag="PLT08_06130"
     CDS             1706..2638
                     /locus_tag="PLT08_06130"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNE04074.1"
                     /translation="MGIGLVSGSGMFFLFLCAGMAIALEIAYNSSSHVFTNNRTSLRM
                     HMKMEHKNRDGNRVLENGRLVYYKEKATREFWERLWSQYTKPDFYKPYQEGHLFEFEK
                     IFNRHLPASGKILEAGCGTAQLVLALSAKGYDCYGLDYTLETLRNTQNLVGPLRLLSG
                     DLHAIAVPDGAFDAIISIGVVEHNYLGPEKILKEMTRVLKKGGVMLISVPYFNSLRKW
                     RADHGAYQDDVLGLEFYQYAFSRREFSDFIVSCGYEIEAVYSYSHQKTLSEELHWLKR
                     VPASLRKLIMRLSKYVPYVNSDLGHMLMVIARKR"
     gene            2711..>4504
                     /locus_tag="PLT08_06135"
     CDS             2711..>4504
                     /locus_tag="PLT08_06135"
                     /inference="COORDINATES: protein motif:HMM:NF025072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNE04075.1"
                     /translation="MIKVFLTGGDEVGWALDEDLNLMRKVLRQMPEISLTDLKNCDIV
                     HSCSPGGLHEISDEQLAGKKVICHMPGEPFRYLQQPDFPKVASKVGLWVARTQQAVNE
                     FKSLNIQSTLVPYIVDEDVFYPLAHDGLEKNALRERLNLPKDSYLIGSFQRDTEGADL
                     ITPKYMKGPDVFFEIVHSLHRKLIPIHVLLAGPRRFWLIKNLEEAGIPYTYVGRHTSK
                     DDIFINTLPRQQLNELYNSLDCYIVSSRSEGGPHAVLEALSSGCSIISTPVGVSLDVL
                     PQDSLFSTVADAVDKVVLDMKRKQSGYASPNPIGIGSTLNLTTLAIHLEQCYRNVHDI
                     KSFKAPDKMDKNAQKSKQTGWLKFPKLIKSGAIKTTTVGLWHTYHKPPYGGGNQFMLA
                     MRKQLALQGVNVIENKIQDMVDYYFLNSIHFDVDAFTRLKKNRDIKVLHRIDGPIYLI
                     RGYDREKDELCYRLNAELASATVLQSTYVYRKIVEFGYQPINPVIIHNAVDPEIFNRK
                     GKLEFDTNRKIRLISSSWSNNPRKGGSIYKWIEEHLNWDLFEYTFVGNVSEPLGKIKH
                     LPPMPSEDLAVVLKEHDIYITASENDPCSNAL"
BASE COUNT         1258 a          850 c         1048 g         1348 t
ORIGIN      
        1 gggtagtttg aatgtctgag atgaaaattg caattccttt gcacggcaag atggcggcgc
       61 gtactttcca taatccatcc ttccgccagc atctggtaag taatggcttt atgcctttgt
      121 acttccttag tccgatatac tatcgctcct ttaatctaaa tcctgagcaa tattttgaac
      181 ttcaaaccga tttttacgat aaagaatatc aaaggcattt tctgttacag caactacgaa
      241 tgttgcgacg ttttgtggtc ataaccgaaa caacagatct gcgctttcgc caaatgattg
      301 aaagtaaatt gtttgattcc tctttgctag gtatgtcatt tcaaatgatg tttgttaact
      361 ttgtaagaaa aatccctgga atggggcaac ttctctctgc tatggagcag aatctgtatg
      421 tttctcatgc acatgaagat cattttgtgc aacaaaatat aggctgtgtt ttgaccccag
      481 gcatgggtaa ttatgcttat tggaatgaag ggaaatttgc gactgaggcg caacggatga
      541 atattcctgt atttgccgcc attaccaatt acgacaatgt tgtcaatatg ggctatcgaa
      601 tgttcacacc caggcgactt gctgtgtgga gtaggcaaat ggctgacgaa attatcaaac
      661 ttcatcgcta ccctgcctcc aaactggagg tgacgggacc ggcacactat gatcgtttta
      721 tgcaaccttt aagtatgagt cgaattggtt tcttggaaaa aataggcttg gacccttcaa
      781 agaaaactgt tttttttgct ggtggcggga acatcagcca ctactttgag atttaccgtc
      841 tgtttgtaga agagaaacac cgtatattac gtggggactt caattttgtt gtccgcccgc
      901 atcaccacgg gcacttaaga acaaaccccg catggaacct tttggaaaaa ctgtttttag
      961 atgcaggagt atatgtatcc aatcccggct cagttgatgc cacgggagat cgaactaatg
     1021 agttgcgttt agatcttgcg ctcgatgaag attatgatga actacattat ttgttgaaat
     1081 acagtgatgt tttggtgaat atattctcca caatgggttt ggaggcggct atttgtgacc
     1141 tgccaacgat tcatattggc tacgatgcct ataccattgg catgaagttt ggcttgacat
     1201 ctgcgtttca acaacggatg acgcataacc gtagaccttt gcgattaaag gcgtctaaag
     1261 ttgcaaagaa cgagatggac ttaataaaat atatcgaaga atatcttggt gaccgcagca
     1321 tggatagtga agtacgcaga gaatatgctg tttctgagtg tggtgagctg gatggattat
     1381 ccagctctag gctcgttgaa atgataaaaa gccatatcta agctggattt tattaaaaaa
     1441 acaaggtaac cttttgttaa tactcctcca agaggtcgct agtagctacc cgtaaaatga
     1501 cttaattccc attttgctag ttgctaggtt gagtggagaa aagagttttt aatattattg
     1561 ccgtgtgtta agttggcgaa aaaattatgg gtacatacga catgtaggga gtgttttatt
     1621 agtgggtgcc ctggtgatgc ctgatgctga ttatgcagcg acttttattt cgcccatgca
     1681 gtagatggag tttgttttgt gggacatggg cattggactt gtgtcgggtt ctggtatgtt
     1741 ttttttgttt ctctgcgcag gcatggcaat cgcccttgag atcgcctaca acagtagttc
     1801 tcatgttttt accaataata ggaccagttt aaggatgcat atgaaaatgg aacacaaaaa
     1861 tcgagatgga aatcgcgttt tagaaaacgg aaggttggtc tattacaagg agaaagccac
     1921 acgggaattt tgggagcgat tgtggtccca atataccaaa cctgatttct acaagccgta
     1981 tcaggaagga catcttttcg aatttgaaaa aatatttaat aggcaccttc ctgcatctgg
     2041 aaaaatattg gaggcaggtt gcggcacggc tcagcttgtg cttgctctca gcgctaaagg
     2101 gtatgactgt tacggtttgg actatacact tgaaactctg cgtaatacac aaaacttggt
     2161 cgggcctttg cggttgttgt ctggcgatct tcatgccata gcggtccctg atggggcttt
     2221 tgatgcaatt atttccatag gtgtggtgga gcacaactat ttgggtcctg aaaaaatttt
     2281 gaaagaaatg acccgtgttt tgaaaaaagg cggtgtgatg cttatatccg ttccatactt
     2341 taactcgttg cgcaaatgga gagcggatca tggtgcgtac caagatgatg tgcttgggct
     2401 ggaattttat cagtatgcat tttccaggag ggagtttagc gattttatcg tttcctgtgg
     2461 gtatgaaatt gaggcagtgt attcatattc acaccaaaaa acgctttcag aagagttgca
     2521 ttggcttaag cgggttccag catcgcttcg taaattgatt atgcgtttga gcaagtatgt
     2581 gccttatgtg aattctgatc ttgggcatat gctcatggtg attgcacgga agcgctaacc
     2641 ggttatgttg gttagagaaa catgatttga gagatgttat ttaacacatc gggacttaag
     2701 gagattattt atgataaaag tttttttgac gggcggtgat gaagttggct gggcgttgga
     2761 tgaagacctc aacctcatgc gcaaggtgct tcgtcaaatg ccagagatat ctcttacaga
     2821 tctaaagaat tgtgatatcg tgcattcctg ttcgccaggc gggctgcatg agatatcaga
     2881 tgagcagctt gcaggcaaaa aagtgatatg ccatatgccg ggcgaacctt tcagatatct
     2941 tcaacagccc gatttcccaa aagtagcctc gaaggtaggc ttgtgggttg ccagaaccca
     3001 acaggcggtc aatgagttta agagtctcaa tatccagagt acattggttc cttatatagt
     3061 ggacgaagat gttttttacc ccctagctca tgacgggctg gagaaaaatg ccctgcggga
     3121 aagattaaat cttccaaagg attcctacct gattggtagt tttcaacgcg atactgaagg
     3181 tgcagacctg atcaccccaa aatatatgaa aggaccagat gtattctttg agattgtaca
     3241 ctccttgcac cggaaattga ttccgatcca tgttcttctg gcggggccaa gacgtttttg
     3301 gctgattaaa aaccttgaag aagcaggtat tccttatacg tatgttgggc gacatacttc
     3361 caaagacgat atttttatca ataccttacc cagacaacag ttgaatgaac tttataattc
     3421 actggattgt tacatagttt ctagccgttc cgaaggcgga cctcatgctg tgcttgaggc
     3481 actttcttct ggatgctcca ttatcagcac tccagtggga gtgtctttgg atgtccttcc
     3541 tcaagatagt ttattttcaa cggttgctga tgctgtagat aaagttgtct tggatatgaa
     3601 acgaaaacaa tcgggttatg ccagtcctaa cccgatcggt attggaagca ctttgaacct
     3661 cactacgctt gctattcatc ttgagcagtg ttatcgaaat gtccatgata ttaagagttt
     3721 taaggcaccg gacaaaatgg ataagaacgc ccaaaagtct aaacagacgg gttggttaaa
     3781 gtttcccaaa ctgataaagt ctggtgcgat taaaacaaca acagtcggtc tatggcatac
     3841 ctatcataaa cctccttatg gtggtggaaa tcaattcatg ctggccatgc gaaagcagct
     3901 tgccttgcaa ggtgtaaatg tgattgaaaa taaaattcaa gatatggttg attattattt
     3961 tctaaactcc atacattttg atgtggatgc ctttactcga ctaaagaaaa acagggatat
     4021 aaaggtacta caccgcattg atggtcctat atacttaatt cgcggatatg accgggagaa
     4081 agacgagctt tgttatcgat taaatgcgga gttggcttca gctaccgtct tgcaatccac
     4141 ttatgtttat cgaaagatcg tggagtttgg gtatcaaccc atcaatccgg ttattatcca
     4201 caatgcagtt gacccggaga tatttaatcg taagggaaaa ctggaatttg ataccaacag
     4261 aaagatcagg ttgatttcat ccagctggtc gaataacccc agaaaaggcg gttcaattta
     4321 taaatggatt gaagaacact taaattggga cttgtttgag tatactttcg ttgggaacgt
     4381 ttccgagccc ctcggcaaaa tcaagcacct tccgcctatg ccatccgaag atctggctgt
     4441 agtgttgaag gaacatgata tttatattac agccagtgaa aatgatccct gttcaaatgc
     4501 attg
//
LOCUS       DAOQJE010000179         4494 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_11564_length_4494_cov_5.054517, whole genome shotgun sequence.
ACCESSION   DAOQJE010000179 DAOQJE010000000
VERSION     DAOQJE010000179.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 4494)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4494)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4494
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11564_length_4494_cov_5.054517"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..470
                     /locus_tag="PLT08_06140"
     CDS             <1..470
                     /locus_tag="PLT08_06140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE04076.1"
                     /translation="FLPHSDSSYIRRFVTPAGESWVDRFYKIRNHSLVDEIYQIEHLG
                     QPKDDDNVYERNSRWAMYSAMGRVGIDNMTLIAVASEFIGDTKDRDIQITRYTIDLMR
                     NLGGRVEEFINPTKYIYSVIDSALERLIEQGIASNEKKITPTKLRKRKAERKV"
     gene            519..1124
                     /locus_tag="PLT08_06145"
     CDS             519..1124
                     /locus_tag="PLT08_06145"
                     /inference="COORDINATES: protein motif:HMM:NF013843.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HNE04077.1"
                     /translation="MNKLFVIVGASGVGKTTLMNSLLTNYDCAIAYEQHDERPFQKLF
                     KEDARYALANQIDYLLMRAEQERELRSGEKPALMDGGFDLDFYGFTRLFHTHGWLSAA
                     EFDLCRRLHTLTRQLLPAPDLIIHLHADAETVRQRLASRNRINIASSEDAELLESYVN
                     EWLETVPGTNILRLDVSNENASYPVSVPRILDKIRGTHGKS"
     gene            1111..1998
                     /locus_tag="PLT08_06150"
     CDS             1111..1998
                     /locus_tag="PLT08_06150"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNE04078.1"
                     /translation="MENLDQLKHQTNGEPKYKLMHANVYVSPGGFHYIDHLDPMEEIS
                     PEIDGSNLTEAEITYIEKSLQSNPERLENQIAAVKRHTIIAGKEALDIGCGGGLFLSK
                     LKAEGANVTGVELSDTRAYYSKTKYGFEVIKRTIEDEYWNAFQGRFDIVTLWDVIEHV
                     NHPLATLQAAAKLLKPGGILLIDTPCRDAFYHRFGQFTYKLSGGKLPTFLNTMYSAKP
                     FGHKQILSLAEMRGLYEAVGMEVVELKRFHELSFPYSFYLKKLVRSDFLVKVLLPFVY
                     IFFAIFPIRNKMLAIGKKA"
     gene            2025..2426
                     /locus_tag="PLT08_06155"
     CDS             2025..2426
                     /locus_tag="PLT08_06155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE04079.1"
                     /translation="MEFEEYINEVTRKTIQEGGSASKPSSCIFLPIQDLWCFPKYPAK
                     TFILSPHINLAEELKNFIKKNITFLDEEDCWLGTWVNPQTGEYYLDISTGIENLEAAR
                     SFAIQAGQKDGREIVAMFNPRQRHTIFLKSV"
     gene            complement(2423..3175)
                     /locus_tag="PLT08_06160"
     CDS             complement(2423..3175)
                     /locus_tag="PLT08_06160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE04080.1"
                     /translation="MQFTGLLWFAFLLVPLVYLQRLLHREIQIIFYLITRNKPLTIAI
                     FSMIFFPGVFLHELSHFLMAKVLGVRTGKFSLLPKSLDTGHLQLGYVETEQTDILRDS
                     LIGLAPIIAGTLFVAYAGVVQLRLDALWHVFSNGQVELFWMGLRLLPTVPDFYLWFYL
                     TFAVSSTMLPSESDRHAWLPLGMWIGALLVLAVFSGAGVWMLNNLAPVFDPLLRSVAL
                     LFALSDIVHIVLLIPLYGIRQVMTYTMQLQVN"
     gene            complement(3179..3934)
                     /gene="tpiA"
                     /locus_tag="PLT08_06165"
     CDS             complement(3179..3934)
                     /gene="tpiA"
                     /locus_tag="PLT08_06165"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HNE04081.1"
                     /translation="MRKTFVAGNWKMNKNIAETRELLSALSSGLNAVNGVERVVCPPF
                     TSLMVASEAFAGTEIGLGAQNLHWEEKGAYTGEIAPGMVKELCNYVILGHSERRTYFG
                     ETNETVNKKLLAAQKFGLTPIVCVGETLAQYEAGETQHVVTSQTREGLKGVSAENAAQ
                     IVIAYEPVWAIGTGKASSAENAQTVHGMIRSVLTEIFGKETAQAIRILYGGSVTAANA
                     AEFFSQPDVDGALVGGASLKPEEFISIAKAAVK"
     gene            complement(3947..>4494)
                     /locus_tag="PLT08_06170"
     CDS             complement(3947..>4494)
                     /locus_tag="PLT08_06170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE04082.1"
                     /translation="SLALWDDGQSVESYEVLAQAANLAGTNKDFFMKAAQEFRQRSAW
                     VSAAGMYLRLVGLGEKENVPPNVVEGLHESVYKSTSDKNMPLYVFFERIDSFDLPLGY
                     IARGRYALYNGSIDDAKLQLSNAEGLRPDMYEVFLLKAEIELKVGSKVTAQNILLSLS
                     SDLGAPDWVRGSAEELLKNNQ"
BASE COUNT         1364 a         1031 c          934 g         1165 t
ORIGIN      
        1 tattcttacc tcactctgat tccagctaca ttcggcgctt tgtaacacca gctggtgaaa
       61 gttgggtgga tcgtttctac aaaattcgaa accattcatt ggtagatgaa atctaccaga
      121 tcgaacacct tggtcaaccc aaagatgatg ataatgtcta cgagcgaaac agccgatggg
      181 caatgtattc agctatgggg cgcgttggca ttgataacat gacgctaata gctgtagcaa
      241 gtgaatttat tggcgatacc aaagaccgtg atattcaaat cactcgctac acaatcgatt
      301 tgatgcgcaa cctgggcgga agggttgaag aatttatcaa ccccaccaag tatatttata
      361 gcgtaatcga tagcgcgctc gaacgcctta ttgagcaagg cattgcctca aatgaaaaga
      421 aaattacgcc caccaagctg aggaaaagaa aagccgaaag aaaggtataa caaaattatc
      481 gacccgctgt aaaagcgggt cgatttatcc taaatgctat gaataaactt tttgtcatcg
      541 taggtgccag cggcgtaggc aagaccacgc tcatgaattc tttgcttacg aactacgatt
      601 gcgccattgc atatgaacaa catgacgagc gtcccttcca aaaattattc aaagaagacg
      661 caagatatgc attggctaat cagatcgact atttattgat gcgggcagaa caagagcgcg
      721 agttgcgatc tggcgaaaag cctgctctga tggatggcgg cttcgatctt gatttttatg
      781 gattcacacg cctctttcat actcatggct ggctcagcgc tgcagaattt gacctgtgtc
      841 gacggctgca tacacttacc cgtcaactgt tacctgctcc tgacctcatc atccatctcc
      901 acgcggatgc agaaactgtc cgacagaggc tggcttcgag aaaccgaatc aacatcgcgt
      961 ctagtgaaga tgcggagctt ttagagtcct atgtcaacga atggctggag acggttccag
     1021 ggacaaatat tctgcggctg gacgtttcca acgaaaatgc gagttatcct gtcagcgtac
     1081 cgcgcatcct tgataaaatt aggggtactc atggaaaatc ttgatcaact taaacaccaa
     1141 acaaatggcg agccaaaata taaattgatg cacgcaaatg tatatgtttc gcccggtggg
     1201 tttcactata ttgaccatct tgacccaatg gaagaaattt caccagaaat cgatggcagc
     1261 aatctaaccg aagctgaaat tacatacatc gaaaaatcat tacaatcgaa ccccgaacgg
     1321 cttgaaaatc aaattgccgc tgtaaaacga catacgataa ttgccggcaa agaggcgttg
     1381 gatattggtt gtggtggcgg gctatttctt tcaaaactta aagcggaagg tgcaaatgtg
     1441 acgggcgtag agttaagcga tactcgcgca tattactcaa aaaccaaata tggctttgaa
     1501 gtaatcaagc gtaccattga ggatgagtat tggaatgctt ttcagggtcg gtttgatatt
     1561 gtcactttgt gggatgtgat cgaacacgta aaccatccat tggcaacttt acaagctgca
     1621 gcaaaattac taaaacccgg cggtatttta ttgatcgaca caccatgccg cgatgctttc
     1681 tatcatcggt ttggccagtt tacatacaag ctctctgggg ggaaattacc gacattctta
     1741 aataccatgt attcagcaaa gccattcggg cataagcaaa ttctatcgct cgcagaaatg
     1801 cgcggattgt acgaagcagt tggcatggaa gtagtagagc taaaacgctt tcacgaactt
     1861 tctttcccat atagttttta cctaaaaaag cttgtacgtt ctgacttttt agtaaaggtt
     1921 ctacttccat ttgtatatat cttttttgcg atatttccta ttcgcaacaa aatgcttgca
     1981 attgggaaaa aggcgtagat ttctttaaaa catagctgct atatatggag ttcgaggaat
     2041 acataaacga agttacaaga aagaccatac aggaaggcgg cagcgcgtcg aaaccgtcat
     2101 cctgtatatt tttaccaatt caagatttgt ggtgttttcc taaatatccc gcaaaaactt
     2161 ttattctctc gcctcatata aatttagcag aagagttaaa aaacttcatc aagaaaaata
     2221 taacgtttct cgatgaagaa gattgctggc ttgggacttg ggtcaacccg caaactggtg
     2281 aatattattt ggatatttca actggaattg aaaaccttga agctgcacgg tcatttgcca
     2341 tacaggcagg tcaaaaagat gggagagaaa tcgttgccat gtttaatcct agacagcgac
     2401 atacaatttt cctcaaatca gtttagttta cttgaagttg cattgtatat gtcatcactt
     2461 gccgaattcc atataaggga ataagcagca caatatgtac gatatcactc agggcaaaca
     2521 acaaggcaac agaacggagt aatgggtcaa atacaggggc aagattattc aacatccaaa
     2581 ctcctgcgcc tgaaaatact gcaaggacta acaaagcacc aatccacata ccaagcggaa
     2641 gccaagcgtg gcgatctgac tcagagggca acatggtgct agaaacggca aaggttaggt
     2701 aaaaccaaag gtaaaaatct ggcaccgttg gcagtaatct caaccccatc cagaacagct
     2761 ctacttgccc gttgctaaac acatgccaca aggcatccaa tcgtagttga acaactccag
     2821 catacgccac aaatagcgta cccgcaataa taggtgccaa gccgatcaac gaatcacgca
     2881 aaatatccgt ctgttcggtc tctacataac ccagttgtaa atgtcctgta tctaaggatt
     2941 taggaagtag agagaatttc cctgtgcgga cacccaaaac tttcgccatt agaaaatgac
     3001 tcaattcatg cagaaatacc cctgggaaaa atatcatcga gaaaatcgca atggtcaatg
     3061 gtttgttgcg ggtaataaga taaaagataa tctgaatttc acgatgcaaa agccgctgga
     3121 gatacaccag cggcacaagt aaaaaagcaa accaaagtaa tccggtaaat tgcacggctt
     3181 atttcacagc tgcctttgca atggaaataa attcctctgg cttcaggctc gcgccgccca
     3241 ccaaggcacc gtcaacatca ggttgggaga aaaattctgc ggcgttggca gcggttacag
     3301 aaccaccata caagatgcgg atggcttgag ccgtttcttt tccaaaaatt tccgtcaata
     3361 cagaacggat catgccatgc acagtctgtg cgttttctgc gctggaagct ttaccagtac
     3421 caatcgccca aacaggttca taggcaatga caatttgagc cgcattttct gcagagacac
     3481 ccttcagccc ctcacgggtt tgcgaagtaa caacgtgctg cgtttcacct gcttcgtatt
     3541 gagccaatgt ttcacctaca catacaatag gcgttaatcc aaatttttga gccgcgagta
     3601 attttttatt cacagtttca ttggtttcgc caaaataggt gcggcgctct gaatgcccaa
     3661 ggatgacata attgcacaat tctttgacca tccctggagc aatctcgcct gtatatgcgc
     3721 ctttctcctc ccagtgcaaa ttctgcgcgc ccaaaccaat ttctgttcca gcaaatgctt
     3781 ccgatgcaac catcagcgag gtaaatggcg ggcaaacaac tcgctctaca ccattgacgg
     3841 cgttcagtcc gctagagaga gcagaaagca gctcacgcgt ctctgcaata ttcttattca
     3901 tcttccagtt tccagcaaca aatgtttttc tcatcgatgc atgctcctat tgattatttt
     3961 ttagtaattc ttccgccgat cctcgaaccc aatcgggcgc acccaagtct gaggatagag
     4021 aaagtaatat gttttgtgca gttaccttgc ttccaacttt caattcaatt tctgccttaa
     4081 gaaggaaaac ttcatacatg tcggggcgta atccttcagc gttcgaaagt tgaagcttgg
     4141 catcatcaat cgaaccattg taaagcgcat atcttccgcg cgcaatatat ccaagcggta
     4201 aatcgaacga atcaatgcgt tcaaagaaaa catacaaagg catgttttta tcagatgtag
     4261 atttatagac agactcgtgt aaaccttcca caacatttgg cggaacattc tccttttcgc
     4321 ctaagcctac caaacgcaaa tacattccag ccgcagagac ccatgcactg cgttgtctga
     4381 attcctgcgc agctttcatg aaaaagtctt tatttgtgcc agccaagttt gccgcttgcg
     4441 ccaagacttc atacgattct acactttgtc catcatccca tagcgctaaa gata
//
LOCUS       DAOQJE010000180         4491 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_11581_length_4491_cov_4.178314, whole genome shotgun sequence.
ACCESSION   DAOQJE010000180 DAOQJE010000000
VERSION     DAOQJE010000180.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 4491)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4491)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4491
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11581_length_4491_cov_4.178314"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..213
                     /locus_tag="PLT08_06175"
     CDS             <1..213
                     /locus_tag="PLT08_06175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE04083.1"
                     /translation="SDNADYRYIDGYRFHLQSAYDFPVSVAPGVTVELTVDMQAPPTP
                     GQYSTFWIINIGKNAFCKMQLDITVQ"
     gene            complement(280..657)
                     /locus_tag="PLT08_06180"
     CDS             complement(280..657)
                     /locus_tag="PLT08_06180"
                     /inference="COORDINATES: protein motif:HMM:NF015953.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HNE04084.1"
                     /translation="MTKFIIRWLINAVALYAAVWLIPGIDYLGDWKGILALALIMGLL
                     NALVRPLLKFLTCPLIILTLGLFTLVINTVMLMLTSSIGQSFGIPLMVDGFWSALLGS
                     LVMSVVSIVMSVIFRDELKGKRD"
     gene            complement(691..1665)
                     /locus_tag="PLT08_06185"
     CDS             complement(691..1665)
                     /locus_tag="PLT08_06185"
                     /inference="COORDINATES: protein motif:HMM:NF024917.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNE04085.1"
                     /translation="MSSITVPARADNHPNLRPMSILRDLPAVADLIEQCFSSTMDSDG
                     RRYVQDMRRAGNDNSFLKWANRVAESTSLPLTGYVWEEGHHIVGNVSLVPFRQNKHRL
                     YLIANVATHPDYRRQGIARALTLRAMQHAHEKKVNDIWLHVRADNPEAIHLYETLGFV
                     ERARRTAWQAYTDANTSQVETDIAITNRYPRFWGTQLKWLSRLYPDALSWHMNWSFSS
                     LKPGLFNWLYLMFLDMNIRQWAATRKDDLLATLSWIPTGRGESLFAATGERSDPEALR
                     ALLTQARRDLSRSYPHISLEFPAGLFDDAIQAAGFKALRTLIWMQATS"
     gene            1785..3926
                     /locus_tag="PLT08_06190"
     CDS             1785..3926
                     /locus_tag="PLT08_06190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE04086.1"
                     /translation="MKKNSMFSRGIDIQVLRVFFLILAIEAIVSFLVLLSLPKSINNA
                     WLFGYSLNRIVLIFVMLLISVILGGFALGLRKHSALQEKSIFILNFLLRSERKFELVL
                     STFSFFFLLVVFLLVLWGVTKDKFYLAYLTRFGPILVFAALFVLQTLWLLLLNYSDNR
                     KSMWYWSLLLGGFLVFLQEWILIINNKIDQPILVGLLLYTTYYTQFVFRKNFNLVLWG
                     SWILASVLIWILIYLEFFFIPKSFLPYKQIFLTFSPLVMTTLAVVTDMVYGVLSWSTH
                     KFWVRVIVFALVLLGVIYVGKAYYTIGTEHAKHVNTAYETFDDELAYMRFVIEAKETN
                     FRYTGMRNQMPIYPYVQALFYEKGMSLDELFAHGKHVNIWLSLLVLGFIFFIAFKFLS
                     LGETINLILITAFGLYALKSGYFMAELLYYALSFSAYLGMCLLLVRPSSKLGITTGIL
                     LGLGHLTKASILPGFLLFVVVFGIKYLALYLWRDKNSLNYEPLKNLSLVALCFLIIIS
                     PYILESKKIYGSYFYNVNSTFYMWYDSYEEAINGTIAHGDGIGWPDMPADEIPSPSKY
                     FKEHSLADIVERVRYGLHWQVENITYQYSFFNYPIFLSAFFLLTLLLNLKVSVQLLRE
                     HWVLVLFVLGYLLAYFSLYVWYSVISSMSRFLFALYLPFVFSVFIAVKELTGKINFPF
                     VKMTNVLVFLMVTIDIWYLLSYGPFFRDFGS"
     gene            3987..>4491
                     /locus_tag="PLT08_06195"
     CDS             3987..>4491
                     /locus_tag="PLT08_06195"
                     /inference="COORDINATES: protein motif:HMM:NF012797.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrmH family RNA methyltransferase"
                     /protein_id="HNE04087.1"
                     /translation="MPKLTISVENAEYQIIQALKLNRTKRHKAQEAFVEGIESIKQVA
                     SAGLEITRIIAVDVERLSTWAKDLIYSHPRAKIVEMSFDLYKALCDRDEPSEIVITVK
                     VNFLNLSDLQLPSNPFVLVFDRPSDMGNLGSILRSANSFGVDAILLIGHGVDVYDPKV
                     IRSSLGSI"
BASE COUNT         1207 a          791 c         1030 g         1463 t
ORIGIN      
        1 tctgataatg cagactatcg ctatatagac ggatatcgct ttcatttaca atctgcatat
       61 gattttcctg taagtgttgc tccaggagtt acagttgaat tgacagttga tatgcaggcg
      121 cctcctacgc caggacagta ttccacattt tggattatta acattggtaa aaatgctttc
      181 tgtaaaatgc aattagatat tacagtgcag taaaacgcat aatttagcct ttaacgaaaa
      241 gagcccttac ttgtaagggc tcttttcgtt atgtattttt tagtctcgtt tacctttgag
      301 ttcgtcgcgg aatatgacgc tcatcacgat actgacaaca ctcattacca aactgccaag
      361 cagggcagac cagaacccat ccaccatgag cggaatgcca aacgattgac cgatagaact
      421 ggtcaacatc aacatgacgg tattaatgac gagggtgaac aagcccaaag taagaataat
      481 aagtgggcag gtgaggaact tgagcaatgg acgcacaagc gcattcaaca accccataat
      541 aagggcaagg gcgagaatgc ctttccagtc gccgagatag tcaataccag ggatcagcca
      601 cacggcagca tataacgcaa cagcgttgat cagccagcga ataataaact tggtcatttt
      661 ttctcctttc ttttatgtac gcaaaatatt ttacgaagtt gcctgcatcc agatcaatgt
      721 gcgaagcgct ttgaagcctg cggcttgaat agcatcgtca aagagaccag cgggaaattc
      781 aagcgagata tgagggtagg aacgagacag gtcgcggcgt gcttgagtga gaagcgccct
      841 caacgcctct gggtcggacc tctcgcctgt tgccgcgaat aggctttctc cacgccccgt
      901 cggaatccac gagagcgtgg cgagcaagtc atctttgcga gtagccgccc attgacggat
      961 attcatatcg aggaacatca ggtacaacca gttgaacaaa cccggtttga gcgaggaaaa
     1021 actccagttc atgtgccaac ttagggcgtc gggatagagg cgggaaagcc atttgagttg
     1081 tgttccccaa aagcgtggat atctattcgt gatggcgata tccgtttcca cttgtgaagt
     1141 gttggcatct gtataggctt gccatgcggt tcggcgggct cgttcgacaa atccaagtgt
     1201 ttcgtagagg tgaatggctt cagggttatc agcacggacg tgtaaccaaa tatcattcac
     1261 ctttttttca tgggcatgtt gcatggctcg cagagtgagg gcacgtgcaa tgccttgacg
     1321 acgataatct gggtgggtag ctacattggc gattagatag aggcgatgct tattttgtcg
     1381 gaagggcaca agactgacat taccaacaat gtggtgacct tcctcccaga catagcccgt
     1441 gagcggtaat gaagtggact ctgctacacg gtttgcccat tttaggaaag agttgtcatt
     1501 gccggcacgg cgcatatctt gcacatatcg cctgccgtcg ctatccatgg ttgaagagaa
     1561 gcattgttca atgaggtctg ctaccgcggg caggtctcgt agaatgctca tagggcgtag
     1621 gttggggtga ttgtccgccc gggcagggac ggtgatggag gacatgaggg gattataagc
     1681 gggttgaggt acaactgctt aaaaagaaat tcttactgcc aattttgaat tgtgcctgca
     1741 tgttacaatt gaaaaatagc agaaataact tccagagaat tacgatgaaa aaaaattcga
     1801 tgtttagccg tgggatagat atacaagttc tacgggtttt ctttttaatt ttagcaatag
     1861 aggcaatagt aagttttctt gttttattaa gccttccaaa atcgattaat aatgcttggc
     1921 tgttcggtta ttcacttaac cgaattgtat tgatctttgt catgcttttg ataagtgtaa
     1981 tattgggtgg gtttgcactt gggctacgga aacattcagc cctacaggaa aaatcaattt
     2041 ttatacttaa ttttctattg cgctccgaaa ggaagtttga actcgtacta agcacatttt
     2101 ccttcttttt tcttctggtc gtgtttttgc tggttttatg gggcgtgacg aaagataaat
     2161 tttatctggc atatctaacg cgttttggac caattctagt atttgcagca ctgtttgtat
     2221 tacaaacctt gtggttgctc ctattgaatt attccgataa tagaaagtct atgtggtatt
     2281 ggtctctgct attgggtggg tttctagtat ttttgcaaga atggattctt attattaata
     2341 ataaaatcga tcagccaatt ctggttggtt tattgttata tacaacatac tatactcaat
     2401 ttgttttccg caagaatttt aatttagtac tttggggaag ttggatttta gcttctgtct
     2461 tgatatggat attaatctat cttgaattct tttttatccc aaaaagtttt ttgccatata
     2521 aacaaatttt cctgacgttt tcgcctcttg ttatgacgac attagctgtc gtaacggata
     2581 tggtttatgg tgtattgtca tggtctacac ataagttttg ggttcgtgta atagtttttg
     2641 ctctagttct attaggcgta atttatgtgg ggaaagctta ttacactatt gggacagagc
     2701 atgcaaaaca tgtgaatact gcctatgaga cttttgatga tgaattagcc tacatgagat
     2761 tcgtaataga agctaaagaa acaaattttc gatatacggg tatgcgaaat caaatgccga
     2821 tttaccccta tgttcaggct ttgttttacg aaaaagggat gagcttggat gaactttttg
     2881 cgcatgggaa gcatgttaat atttggctat cacttttggt tctgggcttt attttcttta
     2941 ttgcctttaa gtttttatct ttaggagaaa caataaactt aatcttaatt acagcgttcg
     3001 ggttgtatgc cttgaagtcc ggttatttta tggctgaatt gttatattat gctttgagct
     3061 tttcagctta tttgggaatg tgccttttgc ttgtaagacc ttcttcgaaa ttaggcatta
     3121 ctacgggaat attgcttggt ttaggacatc ttaccaaggc atctatactg ccaggctttc
     3181 tcttatttgt cgttgtgttc ggaataaaat atttggcgct ttatctttgg agggataaga
     3241 atagtttgaa ttatgagcct ttgaaaaacc tttcgttggt tgctttgtgt ttcttaataa
     3301 taatttctcc atatattctt gaaagtaaga agatatatgg tagctacttt tataatgtca
     3361 actccacatt ttacatgtgg tacgacagtt acgaagaagc aattaatgga acaattgccc
     3421 atggagatgg aattggctgg cccgatatgc cggctgatga aataccaagc cctagtaaat
     3481 attttaaaga acatagtctc gctgatattg tagaaagagt tcgttatggg ttgcattggc
     3541 aggtggaaaa catcacctat cagtatagct tttttaatta ccctattttt ttgtctgcgt
     3601 tttttctttt gacgctttta ttgaatctga aagtcagtgt gcaactgttg agggaacatt
     3661 gggtgttagt gttatttgtt ttaggctatt tacttgcata cttttctttg tatgtttggt
     3721 attcagtgat ctctagcatg tccagattct tgtttgcctt gtacctacct tttgttttta
     3781 gtgtattcat tgcagtaaaa gagttgacag gaaagataaa ttttcctttt gtaaaaatga
     3841 cgaatgttct tgtgttttta atggtaacta ttgatatttg gtatttgctc tcttatggcc
     3901 cgttttttag agatttcggt agttaagact gtaggaaact taaaaataaa aataaatggg
     3961 tagatgccaa gtagtaaaat acgcatatgc ctaaattgac gattagtgtt gaaaacgccg
     4021 aataccagat tattcaagct ttgaaattaa atcgcacgaa gcggcacaaa gcgcaggaag
     4081 cgtttgtgga ggggattgag tcgataaaac aggttgcgag tgctgggctg gagattactc
     4141 gtattatcgc cgtagatgtg gaaagactat cgacatgggc taaggatttg atatatagcc
     4201 acccaagagc caagattgtt gagatgtctt ttgatttgta taaagcatta tgcgaccgtg
     4261 atgagccttc tgagatcgtt ataacggtta aggtgaactt tctcaattta agcgatttgc
     4321 aattaccgtc aaatccattt gtgttagtgt ttgaccgtcc aagtgatatg ggcaatctag
     4381 gctctattct tcgttcggca aattcgtttg gtgtggacgc aattttgctt attggtcacg
     4441 gtgtggatgt gtatgatccg aaagtgattc gttctagttt gggaagtatt t
//
LOCUS       DAOQJE010000181         4436 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_11794_length_4436_cov_7.033326, whole genome shotgun sequence.
ACCESSION   DAOQJE010000181 DAOQJE010000000
VERSION     DAOQJE010000181.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 4436)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4436)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4436
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11794_length_4436_cov_7.033326"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1523)
                     /locus_tag="PLT08_06200"
     CDS             complement(<1..1523)
                     /locus_tag="PLT08_06200"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HNE04088.1"
                     /translation="MTEQFTFGGEIVWRPSQAQVDGANLTAFMQMWKLPDFDALMQKS
                     THDVAWFTEAVLKFLDIRFYEPYSQVIDLKNGIQFPKWCVDGKMNIVHNCVEKWVENE
                     ADRKKIAIAFEGEDGITKTLTYEDVDKEINKTANALRTLGLGKGDAIGIFMPMTPEIV
                     IALLAIAKIGGIILPLFSGYGAGAIVSRMVDADAKALFAADGAFRRGKAVEMKSVADE
                     AAEQIPTLKHMIVLKRTGQDVKMKAGRDHWWHELVDVQNDVCNTEQTSAEDPLMIIYT
                     SGTTGRPKGALHTHCGFPVKAAQDMAFGTDVHQNDVIYWMTDMGWMMGPWLVFGSLIL
                     GATIFLYDGAPDFPTPSRLWELVERHKINQMGVSPTLIRSLIPHGDEYFKKYDLSSLK
                     CFASTGEPWNPDPWMWLFANVGEKRIPIINYSGGTEISGGIVMGNMLLPLKPCAFSAP
                     CPGMDADVVDENGKSVRNAVGELVIKAPWIGMTRGFWKDQQRYLDTYWSRWENVWVHG
                     "
     gene            1672..2214
                     /locus_tag="PLT08_06205"
     CDS             1672..2214
                     /locus_tag="PLT08_06205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE04089.1"
                     /translation="MSEFTMKLIKCPSCGGPIDPPSGESTAKCPYCGSAVVIPESLRK
                     PAVSNQNQSSLTSSANVFGVDLNSMMGYGAQWSQVVQLAQAGQKEEAVKKYMSFSSIS
                     EMDARRTVEGLANTQVYDVSAYTTSVANAYAPMVQSYTDTAKKITAWSFGFSCLITGF
                     IMLTVILILGATFWAVFKSF"
     gene            complement(2262..2678)
                     /locus_tag="PLT08_06210"
     CDS             complement(2262..2678)
                     /locus_tag="PLT08_06210"
                     /inference="COORDINATES: protein motif:HMM:NF016001.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase/quinone reductase family protein"
                     /protein_id="HNE04090.1"
                     /translation="MNGNDFMAWILRSPFHGMLSNGMMIISITGRKTGKKITTPVGYY
                     MEGEYLWVITSRERKWWRNLQGGATVDLVLRRKPVQGFAELELDEKAVEARMSEYLRH
                     IPQAASRMGVRMVDGKPNQEDVARTAKDRLFVRIKL"
     gene            2782..3408
                     /gene="upp"
                     /locus_tag="PLT08_06215"
     CDS             2782..3408
                     /gene="upp"
                     /locus_tag="PLT08_06215"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016292883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004845 - uracil phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HNE04091.1"
                     /translation="MSNVYASKHPLIAHKLSILRDKNTKPKKFRQLVKEIAGLLAYEA
                     TLDLATTPVEIETPLETTTAQELKEKIGLVPILRAGLGMVEGIWELMPSAEVWHIGLY
                     RDEKTLRPVEYYNKLPLEPRVAVCLILDPMLATGGSATATAEVLKRWGVTKIKFVGLI
                     AAPEGIKAMQTAHPDIDIYVAAIDDHLNERAYIVPGLGDAGDRQFGTG"
     gene            3428..3760
                     /locus_tag="PLT08_06220"
     CDS             3428..3760
                     /locus_tag="PLT08_06220"
                     /inference="COORDINATES: protein motif:HMM:NF016854.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TfoX/Sxy family protein"
                     /protein_id="HNE04092.1"
                     /translation="MAYDLKLAERIRAEVKGLPIVEKKMFGGVGYMLHGNMACGILED
                     NLIIRVALDDYEKTLKRKYVKIFTMKNGPRPMKGWVMVEPEGYKTSKQLKDWVKTGID
                     VAINLPPK"
     gene            3784..4326
                     /locus_tag="PLT08_06225"
     CDS             3784..4326
                     /locus_tag="PLT08_06225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE04093.1"
                     /translation="MSSTIALLMFIALIGFIAFLFWRQNRIFNDIQAFYKDNKMVYQE
                     TAPVEHPFIYTSIKPICSKGNLKPDMPFTFMLGSRSEGAGQSRVLHTYIGVFIPDHPR
                     LTDAWLNTWKHKVAERGDNWAQHSDVEPHEKSWGLMGAPEDLPIVAKRINNGVLIAWS
                     GLHIRKVFEARLDELKQTLS"
BASE COUNT         1239 a         1183 c          913 g         1101 t
ORIGIN      
        1 ccatgtaccc aaacattctc ccagcgactc cagtacgtat ccaaatagcg ttgttgatct
       61 ttccaaaagc cgcgcgtcat cccaatccac ggggctttta ttaccaactc gcccaccgca
      121 ttccgaaccg acttaccgtt ctcatccaca acatctgcat ccatgcccgg gcagggagct
      181 gaaaatgcac aaggtttgag tggcaacaac atattgccca ttacaatgcc gccagagatt
      241 tccgtcccgc cagaatagtt gatgatcgga attctcttct cgcccacatt tgcaaacaac
      301 cacatccacg ggtccggatt ccacggctct ccagttgagg cgaagcattt gagcgaagac
      361 agatcatatt tcttaaaata ctcgtcccca tgcggaatca acgagcggat caacgttggc
      421 gaaacgccca tttgattgat tttgtgtctt tctaccaact cccaaagtcg gctcggtgtt
      481 gggaaatctg gcgcaccatc atatagaaat atcgtcgcgc caagaatcaa actcccaaat
      541 accagccatg gacccatcat ccaacccatg tctgtcatcc agtaaatgac atcattttga
      601 tgaacatccg tgccaaacgc catatcctgc gccgctttaa ctgggaagcc acaatgtgta
      661 tgcaaggcac ctttcggtct accagtggtg ccagaagtat aaataatcat caacgggtct
      721 tcagcagagg tttgttcagt attgcaaaca tcgttttgca catcgaccaa ctcatgccac
      781 caatgatctc ttcctgcttt catcttcaca tcttgaccag tccgcttcaa gacgatcatg
      841 tgcttaaggg tcgggatttg ctccgccgct tcatcagcca ccgacttcat ttccacagcc
      901 ttgcctcgtc ggaaggcgcc atccgctgca aatagtgcct ttgcatccgc atctaccata
      961 cgggatacaa tagcgcccgc accataacca gaaaacaaag gcaaaatgat tccgccgatc
     1021 ttcgcaattg ctaataaagc aataacgatc tctggcgtca ttggcatgaa gataccaata
     1081 gcgtcccctt tgcccaatcc taatgttcgc aaagcattgg cagtcttatt gatctcttta
     1141 tctacatctt cgtaggtgag tgtttttgta attccatctt ccccttcaaa tgcgattgct
     1201 atctttttac ggtcagcttc attctccacc catttttcaa cgcaattatg cacaatgttc
     1261 atcttcccat ctacacacca ctttgggaac tgaattccat tctttaggtc aatcacttgt
     1321 gaatacggtt cataaaaccg aatatcaaga aatttcaaga ccgcctcagt gaaccaggca
     1381 acatcatgcg ttgacttttg cattagcgca tcaaaatcag ggagtttcca catctgcatg
     1441 aatgccgtca aattcgcccc atccacctgc gcttgggaag gtcgccaaac gatctcaccg
     1501 ccaaacgtaa actgttcggt catgaacatc tcctcgtcaa gttggaatga agcgattcta
     1561 accccaagcc agccagcgga caatgcaaga agttgactag tacgaatggc acctatgcta
     1621 aaaatctggt gttcatataa tttaaccatt gcaaaccaga ggagaagtgc catgtctgaa
     1681 tttacgatga agctaattaa gtgccccagt tgcggaggtc ccattgaccc gcccagcgga
     1741 gaaagcaccg ccaaatgccc ttactgcggc agcgccgttg tcatcccaga aagtttgaga
     1801 aaaccagcag tctcaaacca aaaccaatcg tctctcacgt catctgcaaa tgtgttcggc
     1861 gtggacttaa attccatgat gggctatggg gcgcagtggt ctcaagttgt ccagcttgct
     1921 caagccggac aaaaagaaga agccgttaaa aaatatatga gcttctccag catcagtgaa
     1981 atggatgctc gccgcactgt ggaaggtctg gcaaatacac aggtatatga tgttagcgcc
     2041 tataccacga gcgttgccaa tgcctatgct cctatggttc aaagctatac tgacaccgcc
     2101 aaaaagatca ccgcctggtc gtttggcttt agctgtttga tcacagggtt tatcatgctg
     2161 accgttatac tgatccttgg ggctacattt tgggcagtat tcaaatcttt ctaaccttat
     2221 aaaagaagcg gatggaattt caaccatccg cttctttctt gttacaactt aattcttaca
     2281 aaaagccggt cttttgctgt tcgagcaaca tcttcctgat ttggctttcc atccaccatg
     2341 cggacgccca ttctgctggc tgcctgcgga atatgacgca gatattcaga catgcgcgcc
     2401 tcaacagcct tttcatccaa ttccagttcg gcaaatccct gaaccggttt acgcctcaac
     2461 accaagtcga cggttgctcc gccctgcaaa ttccgccacc acttccgctc gcggctggta
     2521 atcacccaca aatactcacc ctccatataa taaccaacag gggtagtgat tttcttgcca
     2581 gtctttcgcc cagtgatgct aataatcatc atgccattgc ttaacatgcc atgaaatggg
     2641 gagcgtaaaa tccatgccat aaaatcgttt ccgttcatgt tatctccttt aaaaaaagtg
     2701 taacaaaagt aaccagccga gtcacgagac aaacatcacc ttcacggcta taattggtca
     2761 actcattttg gagaatatgt catgtccaac gtttacgcat ccaaacatcc gttaattgcc
     2821 cacaaacttt ccatcctaag agataagaac accaaaccca agaagttccg ccaactcgtg
     2881 aaagagatcg cgggacttct tgcctatgaa gccacactcg acctcgccac caccccagtt
     2941 gagatcgaaa ccccgctgga aactactaca gcgcaagagc tcaaagaaaa gatcgggctt
     3001 gtgccgattc tacgtgccgg gctgggaatg gtggaaggca tttgggaact catgcccagt
     3061 gccgaagttt ggcatatcgg tttataccgt gatgaaaaaa ccctacgtcc tgtggaatac
     3121 tacaacaaac tcccgcttga gccgcgcgta gcggtctgcc tcattcttga ccccatgctt
     3181 gccaccggtg gttctgccac agccacagcg gaagtgctca aacgctgggg agtgaccaag
     3241 atcaaattcg tagggctgat cgcggcgcca gaaggcatta aagctatgca aacggctcac
     3301 cccgatattg atatctatgt tgctgccatt gatgatcatc tgaacgaacg ggcgtacatc
     3361 gtgcccggct tgggtgacgc tggagaccgt cagttcggaa cagggtaaac gatacaccaa
     3421 gtcacttatg gcatacgacc tgaaacttgc agagcggatt cgcgctgaag taaaaggctt
     3481 acccatcgtc gagaaaaaga tgtttggcgg cgttggctat atgctgcatg gcaacatggc
     3541 atgcggcatt cttgaagata acttaatcat acgtgttgcg ctggatgatt atgaaaagac
     3601 tctcaaacgc aaatacgtca aaatattcac catgaaaaac ggacccagac ccatgaaggg
     3661 ctgggtcatg gtggagccag aaggatacaa aacatcaaaa caacttaagg attgggtaaa
     3721 aacaggcata gatgttgcta ttaacctacc tcccaagtaa atatacataa aaggagattg
     3781 agaatgtctt ccacaatcgc tttattgatg tttattgcgc tcatcggttt tatcgccttc
     3841 ttgttttggc gtcaaaatag aatcttcaac gacattcaag ctttttacaa agacaataag
     3901 atggtctacc aagaaactgc ccctgtagag catcctttta tttacaccag cataaaaccg
     3961 atctgttcaa agggcaacct aaaacctgat atgcctttca cattcatgct tggttccagg
     4021 tctgaaggtg ctgggcaatc tcgagttctc cacacctaca ttggggtctt catcccagac
     4081 cacccacggc tgaccgatgc ctggctcaat acttggaaac acaaagtagc cgagcgtggt
     4141 gataactggg cacaacattc cgatgtggaa ccccatgaaa aaagctgggg actgatggga
     4201 gcccctgaag atttacccat tgttgccaaa cgcatcaaca atggcgttct aattgcatgg
     4261 tcagggctgc atattcgaaa agtatttgaa gcacggctag acgaattaaa gcaaacgctc
     4321 tcctaacaac caaatcatat gggatacaac cccgcttccg actcatttga agcgggttca
     4381 acatcccaaa ctgatactca cactgacaag tgatacccgc agtgatttgc accaca
//
LOCUS       DAOQJE010000182         4415 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_11879_length_4415_cov_5.404817, whole genome shotgun sequence.
ACCESSION   DAOQJE010000182 DAOQJE010000000
VERSION     DAOQJE010000182.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 4415)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4415)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4415
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11879_length_4415_cov_5.404817"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1186
                     /locus_tag="PLT08_06230"
     CDS             <1..1186
                     /locus_tag="PLT08_06230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE04094.1"
                     /translation="EKNTVLFEADAGPWMTILASPESAPINRAVHPLSLILIRPLVRL
                     FSGFMGEHYALGGMIVVSLVAAACVFITWLFVKRATNSSTYAFIFAIFLGSTATHLLF
                     GSLTENYIFGAAALIFFFLLIQSNETRFSVLVPAGLFLFGITITNIAQGVIALFANKL
                     GFKRLIKYSLLILSLGILLTIATNVIYPKYITFFFLPEDIAFEFNFVSPENAGVLSDP
                     IRLSEPSSITRKLNVVSRSILLYGIVAPDVIESISEKPPFPTIDLKTFDVRTKAIASY
                     RGLSNLPLVLWLILLAGAFFMFVKTWRTSKYAPLMLGLLGALGFNFLMHLFYGTELFL
                     YTSYWVYAFVLFIALALSDLAEKTWLEWGLGIILLTLMVNNFHFIFSIFQSLAPFYAA
                     AP"
     gene            1205..1630
                     /locus_tag="PLT08_06235"
     CDS             1205..1630
                     /locus_tag="PLT08_06235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE04095.1"
                     /translation="MTLLRKLIPFVLVAMSLAMAIYQFVLSFSAGEWRNSSEEMVSKW
                     EDRIQSLREALPADVKQVGYIDDAVISEDPALFDANEFQLMQYSVAPVAIDSEKRHEW
                     MIGNFNDDKNLEAWLTEQLGAYELQGFGFGLYLIHDVEN"
     gene            1631..2986
                     /locus_tag="PLT08_06240"
     CDS             1631..2986
                     /locus_tag="PLT08_06240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE04096.1"
                     /translation="MTSLLSFIVLLPRIFLGFFIVHSIWNVKDGKSLLVKIFLSAGVG
                     FGVSSLFSFLWIWVGLPLTVYAWVESILAILLMIWLLFQNREILRIPQVAVKQEFLWL
                     SFLAAGALFFALNLILFGLQYPHGRPDAWINWNVVARFIYLGGSNWQATFLRQWDHPD
                     YPFLVPMTNAITWVFLGNASTWGPVALHFVLSLFAAGLLFAFVNIFRGFKQSILPVLI
                     FTSLPFVVNQGMRQYSDFLVAYLILAAGGLTLMYLQTKEKRIAVLVGLVIGLAGWAKN
                     EGLMAILSVSFVWVLLALKKEGRTGLLRYVIGLVFPVVVIVLFKIFLAPSNDLMSAQE
                     SLADKIFDIERYKTIFSIAIPMLWNLGNTPISIIGLIILTALLVRRSTNRITGLWALA
                     VIVLIQLTAYFGIFVLTPRDLAWHLNTSLDRLYLHILPLAFLLFFLWLKSPQELLSKE
                     S"
     gene            2990..3754
                     /locus_tag="PLT08_06245"
     CDS             2990..3754
                     /locus_tag="PLT08_06245"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III pantothenate kinase"
                     /protein_id="HNE04097.1"
                     /translation="MLLTIDIGNTNLTLGLYEGDTLGAHWRLSTDHNRMPDEYGLQFL
                     GVLQNAGKTIKDIQGISVASVVPPLTGRVIQACREYLKQEPLVVDAGVKTGIKIRYED
                     PKAVGADRVCDAVAVMKLYGGPACVVDFGTATTFNAVTKDGEYLGGAITAGINLAAEA
                     LYTRAAKLPRIDLQVPPSVIGRNTVHAMQSGLLFGYVSMVEGMVNRFRAELGSDMKVV
                     ATGGLAEVVAHETKVIDIIAPWLTLEGLRLIWEMNR"
     gene            3907..>4415
                     /locus_tag="PLT08_06250"
     CDS             3907..>4415
                     /locus_tag="PLT08_06250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE04098.1"
                     /translation="MKTKKIFLTFVIVCTGLAACAPPPLSSTSNGSAPSTDSQPITDS
                     PQSVAPTESETHEAADANHSHTSDVDLTHLELGDGKYSTSPQMGYVYTCQTQFNGGGA
                     TGTGSWMNGDGTWDATKKAVVDGSITWPHSFTITIQGDQRVFVGNDLPDHPTGNYPIS
                     PSDDAYAVDR"
BASE COUNT          972 a         1114 c         1007 g         1322 t
ORIGIN      
        1 ggagaagaac accgtcctct tcgaagccga cgctggtccg tggatgacca tcctcgccag
       61 tcctgagagt gccccgatca accgcgccgt gcatccactg agtctcattc tcatccgccc
      121 attggtgagg cttttctccg gcttcatggg cgaacactat gccctcggcg gaatgatcgt
      181 cgtctcattg gtcgctgccg cctgtgtctt cattacctgg ctctttgtca aacgcgccac
      241 caactccagc acctacgctt ttatcttcgc catctttctc ggctcgaccg ccacacatct
      301 gctctttggt tcattgacag aaaactacat ctttggcgct gcagctctta tctttttctt
      361 tttactgatc caatctaacg aaactcgttt ttcggttctt gtccctgccg ggctctttct
      421 gttcggtatc accatcacca acattgcgca gggagtcatc gccctttttg caaacaaact
      481 gggcttcaaa agactgatta aatattcctt gctcattcta agcctcggca ttttgctgac
      541 cattgccacc aacgtcattt accccaagta catcaccttc ttctttcttc ccgaagatat
      601 tgctttcgag ttcaattttg tttctcctga aaatgcaggc gtcttatccg atcccattcg
      661 gttatcagaa ccttcgtcca ttacccgcaa gttgaacgtc gtttcgcgct ccatcttgct
      721 ctacggaatt gtcgcacctg atgtcatcga atcgatttcc gaaaaaccgc ccttcccaac
      781 cattgacctg aaaaccttcg atgtccgtac taaagccatc gcctcctacc gcggactttc
      841 caacttacca cttgttctgt ggttgatctt gttggcaggc gcatttttca tgttcgtaaa
      901 aacctggcgg acttccaaat acgctccatt gatgctcggc ttgctcggtg ctttgggatt
      961 taactttctc atgcacctgt tttatggcac agagttattc ctttacacct catattgggt
     1021 ctacgctttt gtcttattta ttgctttggc attgtctgac ctcgcagaaa agacttggct
     1081 cgagtggggg cttggaatta ttctcttgac cttgatggtc aataatttcc acttcatttt
     1141 ctcgatcttt caatctcttg ctccctttta cgcggctgcc ccgtagaccg gttaaaatac
     1201 ctgcatgacc ctcctgcgaa agttgatccc atttgttttg gttgccatgt cgcttgcaat
     1261 ggcaatttat caatttgttt tgagcttttc cgcgggtgag tggagaaatt catcagagga
     1321 gatggtctcc aaatgggagg atcggattca gtccctgcgt gaggcgttgc ctgcggatgt
     1381 gaagcaagtt gggtacattg acgatgccgt gatcagcgaa gatcccgcgt tgtttgatgc
     1441 caatgaattc caattgatgc aatattcggt ggcaccggtt gcaattgatt cagagaagcg
     1501 tcacgagtgg atgatcggta atttcaacga tgataaaaac cttgaagcat ggctgacgga
     1561 gcaattaggg gcatatgaac ttcaaggctt tgggtttggc ttgtatctta ttcacgatgt
     1621 ggaaaattag atgacgtcgc ttctgtcatt cattgttttg ctgccaagaa tctttttagg
     1681 ctttttcatc gtccattcca tttggaatgt gaaagatggc aaaagcctgc tggttaaaat
     1741 ctttctgtct gccggggtgg ggtttggcgt ttcatctctc tttagctttt tatggatatg
     1801 ggtcggactt ccattaacgg tctatgcttg ggtcgaaagc attcttgcaa tccttctgat
     1861 gatttggctc ctcttccaaa accgcgaaat tttgcgtatc ccacaggtcg ctgtgaaaca
     1921 agaatttctt tggctttcat ttcttgctgc tggtgctctt ttctttgcgt tgaatctgat
     1981 tcttttcgga ttgcaatacc cacatgggcg ccccgacgcg tggatcaact ggaacgtggt
     2041 cgcgcgtttt atctatctcg gtggttcaaa ctggcaggcg acatttcttc gccaatggga
     2101 tcaccccgat tatccgtttt tggtgccaat gacgaacgcc attacttggg tgtttttagg
     2161 caacgcatcc acttggggac cagtcgcttt acacttcgta ttgtctctct ttgcagcagg
     2221 attgttgttc gcatttgtaa acatttttcg cggctttaaa caatccattt taccggttct
     2281 tatattcact tcgcttccat ttgttgttaa tcagggaatg cgtcagtatt cagattttct
     2341 ggtcgcttat ctcatcttag ccgcaggcgg cttgacctta atgtacctgc aaacaaaaga
     2401 gaaacggatt gccgtccttg ttggtcttgt gattgggttg gcaggctggg caaagaacga
     2461 aggactaatg gcgatcctgt ctgtgtcttt tgtatgggtt ttattggctt tgaaaaaaga
     2521 gggacgaact ggattattaa gatatgttat cggtttggtt ttccctgttg tagtgattgt
     2581 gctctttaag attttccttg ccccttccaa tgacttgatg tctgctcaag aaagcttggc
     2641 ggataagatt ttcgatatcg agcgttacaa aaccatcttt agtattgcaa tccccatgct
     2701 gtggaatctg gggaatacgc ctatcagcat aattggactt ataattttga ccgcacttct
     2761 tgttcgtaga tcaacaaatc gtattacagg tctatgggcg cttgctgtta tcgttcttat
     2821 tcagttgaca gcctatttcg gaatttttgt ccttacccct cgtgatctgg cttggcacct
     2881 gaatacttct ttagatcgtt tgtatttaca catccttcca cttgcatttc ttttgttctt
     2941 cctctggctg aaatctccgc aagaactttt atcaaaagag agttgaccca tgcttcttac
     3001 gatcgacatt ggcaatacca accttacgct tggtttatac gaaggtgata cgcttggcgc
     3061 acactggcgg ctctctacag atcacaaccg tatgcccgat gaatacggct tacagttttt
     3121 aggcgtattg caaaatgccg ggaagaccat caaagatatt caaggcattt cagtcgcatc
     3181 cgtcgtcccg ccgttgaccg ggcgtgtgat tcaagcctgc cgcgaatatc tcaaacaaga
     3241 gccgctggtg gtggatgcag gtgtgaagac cggcatcaag atccgctatg aagaccccaa
     3301 agctgtcggt gcggaccgcg tttgtgacgc agtggctgtg atgaaacttt acggcggtcc
     3361 cgcctgtgtg gtggacttcg gcaccgcgac tactttcaat gccgttacca aagatggtga
     3421 atatcttggt ggcgccatca cagcggggat caatctcgct gccgaagcgc tttacacgcg
     3481 tgcagccaaa ctcccacgca ttgatttgca agtgccgcct tctgtcattg ggcgcaatac
     3541 cgtccatgcc atgcaatccg gactcctctt cggctacgtc tccatggttg agggaatggt
     3601 gaaccgattc cgggcggagt tgggaagcga tatgaaagtc gtcgcaaccg gcgggctggc
     3661 agaagtcgtc gcacacgaaa ccaaagtcat cgatatcatc gccccctggc tcaccctcga
     3721 aggtctgcgc ctgatctggg aaatgaaccg ctagaaatct tgcacctgcc aacgacccat
     3781 gacagccgtc atgggtcgtt gtgattcatc tcatgcttat tgtaaaaaag ctgtgatttc
     3841 acacactctg catgtcaacc cactggctat aaactattgc taatcaattc attagaggag
     3901 aatgacgtga aaacgaaaaa gatattttta acgtttgtga tcgtatgtac tggactggcg
     3961 gcgtgcgcgc ctccgcctct ttcttcgacc tcgaatgggt ctgccccatc aacagactct
     4021 cagcccataa cagattcgcc ccaatctgtt gctccaaccg agagcgaaac ccatgaagca
     4081 gcagatgcaa atcactctca tacttccgat gtggacttaa ctcaccttga acttggtgac
     4141 ggtaaatatt ccaccagccc gcaaatggga tatgtgtaca cctgtcaaac tcaattcaat
     4201 ggcggcggcg cgacgggcac agggtcctgg atgaacggag atggcacctg ggatgcgacc
     4261 aagaaggcag ttgtcgatgg ttccatcacc tggccgcatt ctttcacgat caccatacag
     4321 ggagatcagc gtgtgttcgt tggaaatgac cttcccgacc atcccactgg gaattacccg
     4381 atttcaccca gcgatgatgc ctatgctgta gaccg
//
LOCUS       DAOQJE010000183         4405 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_11926_length_4405_cov_4.825287, whole genome shotgun sequence.
ACCESSION   DAOQJE010000183 DAOQJE010000000
VERSION     DAOQJE010000183.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 4405)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4405)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4405
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11926_length_4405_cov_4.825287"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..412
                     /locus_tag="PLT08_06255"
     CDS             <1..412
                     /locus_tag="PLT08_06255"
                     /inference="COORDINATES: protein motif:HMM:NF012780.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HNE04099.1"
                     /translation="KKGSEVFSISPDATVFEALSQMAKYNTGAMMVVRNGKVEGIVSE
                     RDCVRKMDLEGRSAKTAKVNEIMTSKVIYVEASQQLEECMALMIDKNIRHLPVYDGNE
                     LLGLISVRDVLREVIDVQKFMISQLEHYITGGGR"
     gene            complement(457..1404)
                     /locus_tag="PLT08_06260"
     CDS             complement(457..1404)
                     /locus_tag="PLT08_06260"
                     /inference="COORDINATES: protein motif:HMM:NF012512.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HNE04100.1"
                     /translation="MTVTINDIRQAAQRIKPYIHHTPVMTNESLNQRVGAQVFMKCEN
                     LQKVGAFKFRGASNAVWSLTDIEASSGVVTHSSGNHAQALALAAKIRGIPAYIVMPSN
                     APQVKKNAVAGYGGQITFCEPTLAARESTMESIRQRTGATVVHPYDNEHVIAGQGTAV
                     LELLEDIPDLDVIIAPVGGGGLLSGTSIAATEMKKGIRVIAAEPEMADDAFRSLQAGK
                     IIPSMNPKTVADGLLTSLGALTFPIIKERVEQIVTVSEAGILDAMKFIWERAKIVIEP
                     SAATVIAVLWEKKIDLTGKKVGVILSGGNVDLEKLPWQK"
     gene            complement(1404..2309)
                     /locus_tag="PLT08_06265"
     CDS             complement(1404..2309)
                     /locus_tag="PLT08_06265"
                     /inference="COORDINATES: protein motif:HMM:NF014841.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-2-hydroxyacid dehydrogenase"
                     /protein_id="HNE04101.1"
                     /translation="MYILSKDADEYRSILEKSKLPDLIIDKELTETCEILFGSPFSIR
                     DVLGKLPNLRWVQSTWAGVEPLLDPSLRRDYTLTNARGVFGGLMSEYIFGYLLAHERK
                     IFERMEAQKNKQWDTSLTGSLRGKTIGLLGVGSIGAEVAQTAKVFGMTVRGYTRTSKA
                     SAQVDRYYHGDELLEFVNGLDYLVNILPNTKETKKIIDANTLNALPSHAIFINVGRGS
                     TVDEPALLKVLQENKIAGAVLDVFEQEPLPSEHPFWDAPNLSITFHTSAPSLPEDIAQ
                     VFIENYKLYSDRKPLKHQVDFERGY"
     gene            2351..3286
                     /locus_tag="PLT08_06270"
     CDS             2351..3286
                     /locus_tag="PLT08_06270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE04102.1"
                     /translation="MNSPKIAFRTFMSQILDYAGMYPPANLSLDEAFKNFVAYQSDPY
                     AWMLSRFVIPAKRLKEISAFNEKISFTTLGRGGKDMDEFLANVNLDIADIRTFCDSHQ
                     AATVDMYEVALPASSLTDKFAANDIVTRAADALNKNGIMVFFEAPFVATGESWEVRAE
                     KLLRALRKVKDKHVGFKLRTGGVTADAFPSTAQVAWAMAQTREAGVPLKCTAGLHHPV
                     RHFNQSVQTKMHGFLNVFGAGILTAANGISQAEIQAILEDENAENFVFDENGFSWNTL
                     RVGESEIKKARLFATSFGSCSFDEPKEDLQAIKLL"
     gene            3324..>4405
                     /gene="fahA"
                     /locus_tag="PLT08_06275"
     CDS             3324..>4405
                     /gene="fahA"
                     /locus_tag="PLT08_06275"
                     /EC_number="3.7.1.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004334 - fumarylacetoacetase activity
                     [Evidence IEA];
                     GO_process: GO:0006559 - L-phenylalanine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006572 - tyrosine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetase"
                     /protein_id="HNE04103.1"
                     /translation="MPKIQVGLNSDFPIQNLPYGIFFTKENPTPRVGTRLGDVVIDLA
                     MLDAENAFGKQYGFFADASLNRFMAAGRDVWREIRQRLTRLVAEEQASLKSEALIFIH
                     EVRMHLPVEVGDYTDFYASREHATNVGIMFRGKENALMPNWLHLPVGYHGRASSVVLS
                     GTDVIRPRGQVKPKDAPLDFVASRQLDFELEMGFFVGKGNDLGEPISIEDAHEHIFGM
                     VLLNDWSARDIQAWEYQPLGPFLAKNFATSISPWVVTMDALEPFRVKGPLQDPTPLPY
                     LGSNANGYDIQLEVSLQSARMDAPQVISNSNMKHLYWSIDQMLAHHTITGCNMRVGDL
                     CGTGTISGSTEDSYGSLLELTWRGERP"
BASE COUNT         1138 a          973 c         1018 g         1276 t
ORIGIN      
        1 taaaaaagga agcgaagtgt tctccatttc acccgatgcg actgtttttg aggcgctgag
       61 ccaaatggca aagtacaaca ccggcgccat gatggtagtc cgcaacggga aggttgaagg
      121 aattgtttca gaacgagatt gtgtaagaaa gatggatctg gaggggagat ctgccaagac
      181 agccaaggtc aacgagatca tgaccagcaa ggttatttat gtagaggcga gccagcaact
      241 agaagagtgc atggcactga tgatcgataa gaacattcgc catttgccag tttatgatgg
      301 caatgaactt ctcgggctga tttcggtccg cgatgtgttg agagaggtca tagatgtaca
      361 aaaatttatg atttctcaat tagaacatta cattacaggt gggggaaggt agttatgaaa
      421 tcaagcaccc ttcatttgca gggtgcttga tttattttac ttttgccaag gcagcttttc
      481 taaatctaca ttcccaccac ttagaatcac cccaaccttt tttcctgtta aatcaatttt
      541 cttttcccat aaaaccgcaa tgaccgtcgc cgctgaaggt tcgatcacga tcttggcacg
      601 ctcccatata aatttcatcg catccaaaat ccctgcttca cttactgtga caatttgctc
      661 gacgcgctct ttaatgatgg ggaaagttag agctcctaat gacgttaata acccatccgc
      721 cactgttttg ggattcatcg acggaataat tttaccggct tgcaacgaac gaaatgcgtc
      781 atctgccatc tcaggctcag ccgcaatcac ccgaatccct tttttcattt ctgttgctgc
      841 aatggatgtg ccgcttaaca atccaccgcc accaacaggt gcgataatca catccaaatc
      901 tggaatatct tccagcagtt ccaatactgc tgtcccttga cctgcaatga cgtgttcatt
      961 gtcatatgga tgaaccacgg tcgcgcccgt gcgttgcctg atgctctcca tagtactttc
     1021 tctcgctgcg agcgttggct cgcaaaatgt aatttgtccg ccatatcctg ctacagcatt
     1081 tttcttcacc tgtggtgcat tactcggcat gacgatatat gcaggaatac cacgtatctt
     1141 tgcagccaaa gccaacgcct gtgcatgatt cccagatgag tgagtgacca ctccacttga
     1201 ggcttctata tctgtcaatg accagacggc atttgatgcc ccgcgaaatt tgaatgctcc
     1261 aactttttgc aggttctcgc atttcataaa aacttgtgcg ccaacccttt ggtttaaact
     1321 ttcattggtc attactggtg tatggtgaat gtatggcttg atgcgttgcg ctgcttgtcg
     1381 aatatcattt attgtgacag tcattagtat cctctttcaa aatcaacttg gtgcttcaat
     1441 ggctttctat cactataaag cttgtagttt tctataaaaa cttgggcaat atcttccggt
     1501 aaactcggtg cggatgtatg gaaagttatg ctcagattcg gcgcatccca gaatggatgc
     1561 tcgcttggca aaggctcctg ttcgaaaaca tccaaaactg caccagcgat tttattttct
     1621 tgtagaacct tcaagagcgc aggttcatct actgttgagc cacgccccac gtttatgaat
     1681 atggcatggg aaggtaaagc atttaaggtg tttgcatcta taatcttttt ggtttctttc
     1741 gtgttcggaa gaatattgac gagataatcc aatccgttca caaattcaag caactcatcc
     1801 ccatgataat acctatctac ttgtgcactt gctttgctgg ttcgtgtata tccgcgcacg
     1861 gtcatgccaa agacttttgc agtttgcgct acttctgcgc caattgagcc aactcctaat
     1921 aacccaatcg tttttccacg caacgaacca gtgagggatg tgtcccattg cttgtttttt
     1981 tgtgcttcca ttcgctcaaa gatcttccgc tcgtgtgcta gaagatatcc aaaaatgtat
     2041 tcagacatta atccgccaaa cacacctctc gcattggtta atgtgtaatc cctccgaaga
     2101 gatggatcta acagaggctc tacacctgcc catgttgatt gcacccaacg caagtttgga
     2161 agtttcccca acacatcacg aatagagaat ggagagccaa aaagaatctc gcaggtttca
     2221 gtaagctctt tatcgattat taagtcaggt agctttgact tttctaatat tgatcgatat
     2281 tcatctgcat ctttgctaag aatatacatg tgtggcatag tgccccaatt ttacccgcta
     2341 taatacagat atgaattctc cgaaaatagc tttccgtaca ttcatgtcgc aaattttgga
     2401 ttatgccgga atgtacccgc ctgcaaattt gtcgctagat gaagctttca agaattttgt
     2461 tgcttatcaa tccgatcctt atgcgtggat gctgtcacgg ttcgtgattc ccgccaaacg
     2521 cttgaaggag atttcagcct tcaatgaaaa aatatcgttt acaactctag ggcgtggagg
     2581 caaggatatg gacgaatttc ttgccaatgt gaacttggat attgcagaca tccgcacatt
     2641 ttgtgactct caccaagccg ctacggtaga catgtacgaa gtagccttgc ccgcttcttc
     2701 cttgaccgat aaatttgcag ccaacgatat tgttactcgc gccgccgatg ccttaaacaa
     2761 gaacggcatc atggtctttt ttgaggctcc ttttgttgcg actggagaaa gctgggaagt
     2821 acgagcggag aaattgctac gtgccttgcg caaagtaaaa gataaacatg ttggctttaa
     2881 acttcgcacc ggcggtgtga cagcggacgc attcccatct actgcgcaag ttgcatgggc
     2941 gatggctcaa acccgtgagg cgggtgttcc gctcaagtgt acggctgggc tgcatcatcc
     3001 tgttcgacat ttcaatcaaa gtgtgcaaac caaaatgcat ggctttctga acgttttcgg
     3061 ggcagggata ttgacggctg cgaacggaat ctcacaggca gagattcaag ctattttgga
     3121 agatgaaaac gctgaaaact ttgtctttga tgaaaatgga ttttcttgga atactttgcg
     3181 tgtaggcgaa tcagagataa agaaggcaag attgtttgcc acgtcttttg gtagttgcag
     3241 tttcgatgag cctaaagaag atttgcaagc tataaaactt ttgtagtttc agttaacaac
     3301 ctcatttata aggtgaaaca actatgccga aaattcaagt tggtctaaat tctgatttcc
     3361 caattcaaaa tttaccttat gggatattct ttacaaaaga gaatcctacc ccacgtgtgg
     3421 ggacacggtt aggtgatgtt gtcattgatc ttgccatgtt ggatgctgaa aatgcctttg
     3481 gtaagcaata tggcttcttt gcagatgcat cgcttaaccg cttcatggcg gcaggacgag
     3541 atgtgtggcg ggaaattcgt cagcgtttga ctaggctagt ggcggaagag caggcatccc
     3601 ttaagagtga agcgttgatt ttcatccacg aagtacgtat gcatttaccc gttgaggttg
     3661 gtgattacac tgatttttac gcatctcgcg aacatgctac gaatgtgggg attatgttcc
     3721 gtggaaaaga aaatgcgctt atgccgaatt ggttgcattt accagttggg taccacgggc
     3781 gcgctagttc ggtggtgctt tcgggtacgg atgtgattcg tccgcgtgga caggtgaaac
     3841 cgaaagatgc gccgttggat ttcgttgcat cgcgtcagtt ggattttgaa ttggagatgg
     3901 gcttttttgt ggggaaggga aatgatcttg gtgagcctat ttcaattgaa gatgctcatg
     3961 agcatatctt tggcatggtg ttgctcaatg actggagcgc gcgtgatatt caggcgtggg
     4021 aatatcagcc tttaggacca ttcctcgcca agaattttgc tacatctatt tcaccttggg
     4081 tagtaacgat ggacgctttg gaaccattcc gcgtgaaagg accactgcaa gacccaaccc
     4141 cgttgccgta tcttggctcg aatgcaaacg gatacgatat tcagttggaa gtgtctttgc
     4201 aatctgcccg aatggatgcg ccgcaagtca tcagtaattc gaatatgaag catttgtatt
     4261 ggagcattga tcagatgctg gcgcatcaca ccatcacggg ttgtaatatg cgtgtgggag
     4321 acttatgtgg cactggcacg atcagtggat cgacagaaga tagctacggc tcactgcttg
     4381 agttaacttg gcgcggcgag agacc
//
LOCUS       DAOQJE010000184         4388 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_11992_length_4388_cov_7.619432, whole genome shotgun sequence.
ACCESSION   DAOQJE010000184 DAOQJE010000000
VERSION     DAOQJE010000184.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 4388)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4388)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4388
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11992_length_4388_cov_7.619432"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..255
                     /locus_tag="PLT08_06280"
     CDS             <1..255
                     /locus_tag="PLT08_06280"
                     /inference="COORDINATES: protein motif:HMM:NF015126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EF-Tu/IF-2/RF-3 family GTPase"
                     /protein_id="HNE04104.1"
                     /translation="VLQVFTASKFGRVAGCRVTDGELKRGAKVRLYRDSDIIYEGDLS
                     SLRHEKDDVKEIRQGFECGVGFKNFSDIQSGDTLVCYILE"
     gene            326..676
                     /gene="rbfA"
                     /locus_tag="PLT08_06285"
     CDS             326..676
                     /gene="rbfA"
                     /locus_tag="PLT08_06285"
                     /inference="COORDINATES: protein motif:HMM:TIGR00082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HNE04105.1"
                     /translation="MPSGIRIKRIEDRIQQELSEMLIHEISDPRLKQIYVTGIKVDRE
                     LAFAEVYVSAVEGASRSEEVLAGLESASGFLRRTLSARVELRSFPKLRFHWDPTPENA
                     DHIEKLLAGLRNKK"
     gene            716..1672
                     /locus_tag="PLT08_06290"
     CDS             716..1672
                     /locus_tag="PLT08_06290"
                     /inference="COORDINATES: protein motif:HMM:NF013528.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HNE04106.1"
                     /translation="MTDQISGDIKNRLADAKKVVIASHVRPDGDAIGSLLGLGLALIE
                     AGKTVEMILVDGAPASFKFLEGSELIKKEPTGGHDTFITVDCADFKRTGKIFESFGQP
                     DINIDHHKTNEKFGKLNLIEAEEVATAAILTNHLPTWGLKITKPVAAALLTGILTDTL
                     GFRTSNVTPEALRQAATLMESGVDLPEIYMQSLVRKSFPAAKYWGAGLSSLESKDGIV
                     WGTLTLEDRTSASYGGNDDADLINMISAIEGHKVGMVFVQQTDNKVKISWRALQAGID
                     VSPIAKHFNGGGHAAAAGADIEGSLADIQKQALTVTKELLKL"
     gene            1765..2694
                     /gene="truB"
                     /locus_tag="PLT08_06295"
     CDS             1765..2694
                     /gene="truB"
                     /locus_tag="PLT08_06295"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: protein motif:HMM:TIGR00431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004730 - pseudouridylate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HNE04107.1"
                     /translation="MNSQDVKNAISGALVIDKPVGMTSHDVVQAIRNGTGLRRAGHTG
                     TLDPRASGVLVILVGPAVRLSEYVSASDKRYQAIIRLGGTTDTYDAEGKFTPTKDPSH
                     ITEAEFEEALKTFVGEIEQTPPPYSAVKVQGRKAYEMARKGEEVELEPRTITVHHLEV
                     LEWTPPEVVIDVHCSSGTYVRSLANDLGKKLGCGAYLVGLRRTKSGKFTLRDAVPLRK
                     LQEAFNAGNWYQYLIPAAEALGDWPAIELNPDEVEAVRHGHRVVAKASDIVHEKVRGV
                     STQGELVALMVQADNVETGNKEWQPKKVFFTSD"
     gene            2741..3667
                     /locus_tag="PLT08_06300"
     CDS             2741..3667
                     /locus_tag="PLT08_06300"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: protein motif:HMM:NF004160.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003919 - FMN adenylyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HNE04108.1"
                     /translation="MQHYRSLEEVSLKNSYLTVGVFDGVHRGHREIIQKLVQDAHANH
                     APAVVLTFSPHPATVLTGREIHCLTTPDERADLLGSLGVDAVITQRFTRDLSTVPAHE
                     YMSSLKTHLGLSHLLIGYDFALGKGREGNAPRLAEIGTELGYTVEVVPALGDESGVIS
                     STEIRKLVSIGNMPEASKLLGYPYRMSGEVIHGAGRGKQINFPTANVDYAPQKASPPN
                     GIYACWAKLGDERFMAATNIGFNPTFTPERKTQSLEAYLLDFDRDIYGDVLTLEFVAR
                     LREEIRYTNVEALIEQIHDDVLQTRKLLSDLK"
     gene            3710..4147
                     /locus_tag="PLT08_06305"
     CDS             3710..4147
                     /locus_tag="PLT08_06305"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HNE04109.1"
                     /translation="MPKRSGIILIHEGKLALIERYKKGRHYFAFPGGGVDEGETDEQA
                     AIREAEEELGIRVKIVQKAATVLRTGRRDQVYFVVNWVDGEFGTGTGEEYTEPYEENG
                     TYAPLWMPLEDVLVKNVVPRGLAELTVRSHQEGWKENVIIHES"
BASE COUNT         1228 a         1022 c         1079 g         1059 t
ORIGIN      
        1 gtcttgcagg tctttactgc ctcgaagttt ggacgtgtgg caggctgccg tgtaaccgac
       61 ggcgaattga agcgtggtgc caaggttcgc ctttaccgcg actcagatat catctacgaa
      121 ggtgacctct cctctcttcg ccacgaaaaa gacgatgtga aagagatccg ccaagggttt
      181 gaatgtggtg ttggtttcaa gaacttctcc gacatccaat ctggcgacac cctcgtttgc
      241 tacatcttgg aataagtata cttactttta ctacttacat atcacgtatc aaagtgatat
      301 gtaagtagta acatttggaa agactatgcc atctggaatt agaatcaaac gaattgaaga
      361 tcgtattcag caggaacttt cggaaatgct gattcatgag atcagtgatc cgcgtctcaa
      421 gcaaatttat gtgaccggca tcaaagtgga ccgtgaactt gcctttgcgg aagtgtacgt
      481 ctctgccgtg gaaggggctt cccgctctga agaagtgctg gcagggctag aatccgcttc
      541 tgggtttctg cgccgcaccc tttctgcccg tgtagaacta cgttcattcc caaaattacg
      601 atttcattgg gatcccacac ctgaaaatgc tgaccatatc gaaaaactgt tggcagggtt
      661 aaggaacaaa aaataatcat ccttattgaa aataaggaat tgtggagaaa ttatagtgac
      721 agatcagatc tcaggggata taaaaaatcg actcgcagat gctaagaagg tggtgattgc
      781 atcgcatgta cgcccagacg gagacgccat cggctccctg ctcggacttg gactggctct
      841 aatcgaagca ggcaaaaccg tcgaaatgat cctggtagat ggagcgcccg cttctttcaa
      901 atttcttgaa gggagtgaac tcattaaaaa agaacccacc ggcggacacg acacttttat
      961 tactgtagat tgtgccgatt tcaaacgaac ggggaaaatc tttgaaagct ttggtcagcc
     1021 tgatatcaat attgaccacc acaaaaccaa cgaaaaattt gggaaactaa atctaattga
     1081 agcagaagaa gttgccacag cagctattct caccaaccac ctccccactt gggggctcaa
     1141 gatcaccaag ccggttgccg ctgctttatt gaccggtatt ctgaccgata cccttggctt
     1201 ccgcacatcg aacgtcacgc ctgaagcgtt gcgtcaagct gcgactttaa tggaatcggg
     1261 tgtggacctt ccagaaattt acatgcaatc gctagtgaga aaatccttcc cggctgccaa
     1321 gtattggggc gccgggcttt caagccttga aagcaaagat ggcattgttt ggggaacgct
     1381 gacattggaa gaccgtacat ctgcaagtta tggcggcaac gatgacgcag atttgatcaa
     1441 tatgatctct gccatcgaag gacataaagt gggaatggtc tttgtgcaac agaccgataa
     1501 taaagtaaag atctcctggc gtgctcttca ggcgggcatt gacgtttcgc cgatcgccaa
     1561 acacttcaac ggtggcggtc atgcagccgc tgcaggagca gatattgagg gaagtttagc
     1621 tgatattcaa aaacaagcat taactgtcac caaagaacta ttgaaattgt aagtacggta
     1681 gtactacagc actatgttat atgcattgaa aaaaataccg aatatgtcat aataaaaagg
     1741 acaatgaagt aggaggattg taagatgaat agtcaagatg taaagaacgc catatcaggc
     1801 gcgctcgtta tcgataaacc cgtgggtatg acctctcacg atgtagtaca agccattcgc
     1861 aatggcacag ggcttcgccg cgcagggcat actggtacat tagaccccag agcttctggc
     1921 gtactcgtta tattagtggg tcccgccgtt cgtttaagtg aatatgtttc tgcatccgat
     1981 aaacgctatc aagccatcat ccgcttgggc ggtacgaccg atacctacga tgccgaagga
     2041 aagttcaccc ccaccaaaga cccttctcat atcactgaag cggaatttga agaagcattg
     2101 aaaacatttg tgggtgaaat cgagcaaacc ccgcctccct attctgccgt taaggtacag
     2161 ggacgcaaag cctacgaaat ggcgcgcaaa ggcgaagaag tagaactcga acctcgcacc
     2221 attaccgttc accacctcga agtgctcgaa tggactcccc ccgaagtggt gatcgatgtt
     2281 cattgctcgt ccggaacgta tgtccgttcg ctggcgaatg acctcggtaa aaaattaggc
     2341 tgtggcgctt atctcgttgg cttacgccgc accaagagcg gaaagttcac cctgcgcgat
     2401 gccgttccgc tacgcaaact gcaagaagcc ttcaatgcag gcaattggta tcaatacctc
     2461 atccctgcgg ctgaagcgct cggcgattgg cccgctattg agctcaaccc cgacgaagtg
     2521 gaagccgtac gtcatggtca tcgcgtggta gcaaaagcct cagacatcgt acacgaaaaa
     2581 gtgcgcggcg tcagcaccca aggtgaattg gttgcactca tggttcaggc agataatgtc
     2641 gaaaccggca acaaagaatg gcaacctaaa aaagtgttct tcacgtctga ttaacacgga
     2701 atgataaaat aaagccgccg tcaaaggcgg cttttttcat atgcaacatt accgctctct
     2761 tgaagaagtt tccctaaaaa attcatatct aactgtgggt gttttcgacg gtgtccaccg
     2821 gggtcatcgc gagatcattc aaaaactggt acaagatgct catgccaacc atgccccagc
     2881 tgtggtgtta accttttcgc cgcacccggc aacagtactc acaggcagag aaatccattg
     2941 cctaacaaca cctgatgaac gagcggattt gctcggttcc ttgggtgtgg atgcggttat
     3001 cacccaacgc ttcacacggg acctttcaac tgtccctgcc catgaatata tgtccagtct
     3061 gaaaactcac ctcgggctta gccatctgct aattggttat gattttgccc tcgggaaagg
     3121 acgtgaaggc aatgccccac gtctcgccga gattggcacg gagttaggat acaccgtcga
     3181 agttgtacca gctttgggcg acgaaagcgg cgtgatctca tccaccgaga ttcgcaagct
     3241 ggtttccatc ggcaatatgc ccgaagcatc caaactgctt gggtatccct accgcatgag
     3301 cggagaagtc attcacgggg ctgggcgcgg aaagcaaatc aacttcccca ctgcgaatgt
     3361 cgattatgct ccccagaaag cgtctccacc caatggtatt tatgcttgtt gggcaaaact
     3421 gggcgatgaa cgttttatgg cagcaacaaa tattggcttc aaccctacat ttacccctga
     3481 gcgaaaaact cagagcttgg aagcctattt actcgatttt gaccgtgata tttatggtga
     3541 cgtattgact ctggaatttg ttgcccggct acgggaagaa atacgttaca caaacgtaga
     3601 agcgctgatc gaacagattc atgatgatgt gctgcagacc cgtaagctat tgagtgattt
     3661 gaaataatcg ggcaattgcc cgatattttt tgcgtttcaa aggagtgata tgcctaaacg
     3721 ttcaggaatt attttgattc atgaaggaaa gctggcactg atcgaacgct acaaaaaggg
     3781 gcggcattat tttgcttttc ccggcggcgg cgtagacgag ggcgaaactg acgaacaagc
     3841 tgccattcgt gaagcggaag aggaattagg cattcgggtg aagatcgtgc aaaaggctgc
     3901 tacggtctta cgcacagggc ggcgcgacca ggtgtatttc gtggtgaact gggtggatgg
     3961 ggaatttggc acgggaacgg gtgaagaata tacggaaccc tatgaggaaa atgggacgta
     4021 tgccccgctt tggatgcctt tggaagatgt gctggtcaaa aacgttgtgc cgcgtgggct
     4081 tgccgagcta acggttcgat ctcaccaaga gggttggaaa gaaaacgtaa tcatccacga
     4141 gtcttaagtc aaaacgccgc aaaagcggcg ttttttattt tcagttcaaa atccactctt
     4201 aagtattaga agtggcaggt ttattttttg tatgatgaaa gaaacccctt tttatatgga
     4261 tacgccgaat ctgtataaag aaatgaaagg aggaaagccc atgacctagg aactttttcc
     4321 accacaggta cgtcacttgt acaacgccga aattcaaaaa ttaaatacgg aggatgaaat
     4381 gaaatcac
//
LOCUS       DAOQJE010000185         4386 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_11999_length_4386_cov_10.099977, whole genome shotgun
            sequence.
ACCESSION   DAOQJE010000185 DAOQJE010000000
VERSION     DAOQJE010000185.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 4386)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4386)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4386
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11999_length_4386_cov_10.099977"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            21..326
                     /locus_tag="PLT08_06310"
     CDS             21..326
                     /locus_tag="PLT08_06310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE04110.1"
                     /translation="MYGTIARFRVRKDVNKEEFKQKMDEFGSAIIPGWIADYIFQTDA
                     SSNEYFLVAIFKDKATYQANADSAEQHERYLIFRSFLEDDPEWNDGTIVSATGPGSK"
     gene            complement(393..776)
                     /gene="rplL"
                     /locus_tag="PLT08_06315"
     CDS             complement(393..776)
                     /gene="rplL"
                     /locus_tag="PLT08_06315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HNE04111.1"
                     /translation="MSDKLAKLVDELSTLSVLEAADLVKMLEEKWGVSAAAPVAVAAV
                     GGGAAAAAEPVEEKTEFDVIIKDAGAKKIDVIKVVRQLTSLGLGEAKALAEAAGSKVL
                     TGVGKDAAMDAKKKLEEAGAQVVVE"
     gene            complement(851..1375)
                     /gene="rplJ"
                     /locus_tag="PLT08_06320"
     CDS             complement(851..1375)
                     /gene="rplJ"
                     /locus_tag="PLT08_06320"
                     /inference="COORDINATES: protein motif:HMM:NF000955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HNE04112.1"
                     /translation="MAISKQRKEEVLAQYNDWVKRSQAVILVEYSGANMKALDGLRAK
                     IRESGGEFHVVKNTLARRVFADNGMEVPGDMLVKSTAVSFAFSDPASTAKALTDATKG
                     SEFVKVKGGFMAGKPLNAAQVKALSDLPPLPVMRATLLGVLQAPAGKLVRTLAEPARG
                     LAAVIKARSEQAAA"
     gene            complement(1600..>1943)
                     /locus_tag="PLT08_06325"
     CDS             complement(1600..>1943)
                     /locus_tag="PLT08_06325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009256176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HNE04113.1"
                     /translation="VGRLGRVLGPRGLMPNPKAGTVVGASDMDRAIKEAKAGRVEYRL
                     DKSNNIHVSIGKASFDATKLYENYVALMDAINKSRPTGAKGNFVKRITVASTMGPGVK
                     VDPNEHIDGEG"
     assembly_gap    1944..2043
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2044..2468)
                     /locus_tag="PLT08_06330"
     CDS             complement(<2044..2468)
                     /locus_tag="PLT08_06330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HNE04114.1"
                     /translation="MAKHGKKYTAALAKVDIEKIYAAKEAVALAKETSITKFDSTIEV
                     HMRTTLDSRQSDQQLRDVVVLPHGLGKTVRVLVFAQGEGAVAARAAGADFVADDDESL
                     KKIEGGMLDFDVAIATPDAMGKVGRLGRVLGPRGLMPNP"
     gene            complement(2521..2946)
                     /gene="rplK"
                     /locus_tag="PLT08_06335"
     CDS             complement(2521..2946)
                     /gene="rplK"
                     /locus_tag="PLT08_06335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004118588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HNE04115.1"
                     /translation="MAKKLKAIVRLQLEAGKANPAPPVGPALASHGINIMAFCKEYNA
                     RTSNRPGEVLPAEITIYTDGSFTFVLRTSPAAVLLRKAAKIDKGSGVPNKEKVGKVTR
                     AQVKEIAELKMKDLNAISLEGAMLQIEGTARSMGITIVD"
     gene            complement(3049..3579)
                     /gene="nusG"
                     /locus_tag="PLT08_06340"
     CDS             complement(3049..3579)
                     /gene="nusG"
                     /locus_tag="PLT08_06340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HNE04116.1"
                     /translation="MVEGPAWFVIHCYSGYENKVRHNLEQRIETMGMKDMIFDVVIPT
                     QEEIEVKDGKRRTVERHIFPGYVLVNLILTEESWYVVRNTPGVTGFVGMGNSPTPLRP
                     EEVAQIVKRMEAESPTVKVTFKQGERVRIIDGPFNDFRGVVSEIDMERTKVRVMVSFF
                     GRETPVELDFLQVEKS"
     gene            complement(3753..3971)
                     /gene="secE"
                     /locus_tag="PLT08_06345"
     CDS             complement(3753..3971)
                     /gene="secE"
                     /locus_tag="PLT08_06345"
                     /inference="COORDINATES: protein motif:HMM:TIGR00964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HNE04117.1"
                     /translation="MAEKAKQVKKKENAVQRYFRETTGELRKVSWPTWPEARRLTILV
                     LIVMVVMGLFLASVDALAKELLNLMLGV"
     gene            complement(4033..4105)
                     /locus_tag="PLT08_06350"
     tRNA            complement(4033..4105)
                     /locus_tag="PLT08_06350"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4070..4072),aa:Trp,seq:cca)
     gene            complement(4139..4309)
                     /gene="rpmG"
                     /locus_tag="PLT08_06355"
     CDS             complement(4139..4309)
                     /gene="rpmG"
                     /locus_tag="PLT08_06355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HNE04118.1"
                     /translation="MASKKKDVRPVITLQCNDCKERNYTTEKNRRNDPNRMELKKYCD
                     RCKKHTVHRETK"
BASE COUNT         1028 a         1156 c         1055 g         1047 t
ORIGIN      
        1 tacaagaaaa gaggtgactt atgtacggaa ccatcgctcg ttttcgtgtc agaaaagatg
       61 taaacaagga ggaatttaag caaaaaatgg atgagttcgg ctcagccatc atccccggct
      121 ggatcgcaga ctatatcttc caaaccgatg ccagctcgaa tgaatatttc ctcgtagcaa
      181 tctttaaaga taaagcaacc tatcaagcaa atgctgattc tgcagaacag catgaacggt
      241 atttgatctt ccgttcattt ctggaagacg accccgaatg gaatgatggg acgattgttt
      301 cagcaaccgg tccagggtcc aagtaaagaa gcgcatgaat caaaaaagga ctgcccgaaa
      361 tcggacagtc cttttttgtt ccgagtagta gattactcga cgacgacctg agcgccagct
      421 tcttcgagct tcttcttggc gtccatggcg gcatctttgc ccacaccggt gagaaccttg
      481 gaaccagcag cttcggcaag agccttggct tcgccgagac cgagggaggt caactggcgg
      541 acgaccttga tgacgtcgat cttcttggca ccagcgtcct tgatgatcac atcgaattcg
      601 gtcttttctt caacaggttc agcagcggcg gcagcaccac caccaacggc agcaacagca
      661 accggagcag cggcagaaac gccccatttt tcttcgagca tctttacgag gtcagcagcc
      721 tcgagaacgg aaagggtgga aagttcatcc acgagtttag cgagcttgtc agacattgtt
      781 tagcactcct tgctaagtaa ttagataatt ttgattaacg gtgcgagttt tggaactcgc
      841 ttggataaaa ctaggcagca gcctgttcgg aacgtgcctt gatgacggca gccaatccgc
      901 gagcgggttc tgcgagcgtg cggacgagtt tgccagccgg ggcttgcaaa acgccgagca
      961 atgttgcgcg cataacgggc agcggcggca ggtcagacaa agccttcacc tgagcagcgt
     1021 tcaacggctt accagccatg aaaccaccct ttaccttcac aaactcgcta ccttttgtag
     1081 catcggtcag ggcttttgcg gtggaagcag gatctgagaa cgcgaacgag acagcggtag
     1141 acttgacgag catgtcaccg gggacttcca tgccattgtc agcgaaaacg cggcgagcca
     1201 acgtattctt gacaacatgg aattcaccac cagattcacg gatcttcgca cggagaccat
     1261 cgagcgcttt catgttagcg ccagagtact ccacgagaat caccgcctgg ctgcgcttga
     1321 cccagtcgtt atactgagcc aaaacttctt ccttgcgttg ctttgaaatt gccaaaggga
     1381 attcacctcc tttcaaataa aaagtctttg ccactctcgc aggcaaagac tttttcgcac
     1441 tccctcacgg gagaacagca cggaggtgct gaaatatgct actgggtctc cacctgagca
     1501 ggatattaag ttccgcttgg gtcacgtact ccgcacgacc atttgcagaa cacccgccgt
     1561 cattggcgaa gtggggttag gaattatagg ataacgtgct tatccttctc catcaatatg
     1621 ttcgttggga tcgaccttga cgccgggtcc catggtggaa gcaacggtaa tgcgcttcac
     1681 aaaattgcct ttcgcaccgg tcgggcgaga cttgttgatc gcgtccatca gagcgacgta
     1741 gttttcatac agtttggttg catcgaaaga agctttaccg atagaaacgt ggatgttatt
     1801 gctcttatcg aggcggtatt ctacacgacc agccttggct tccttgatgg cgcgatccat
     1861 atcagaagcg cccacaacag taccagcttt ggggttcggc atcaagccgc gcggaccaag
     1921 cacacgaccc aagcgaccaa cctnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1981 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2041 nnnttggggt tcggcatcaa gccgcgcgga ccaagcacac gacccaagcg accaacctta
     2101 cccatcgcat caggggtagc gatggcgaca tcgaaatcaa gcatgccgcc ttcgatcttc
     2161 ttcaaagatt cgtcatcgtc cgcaacgaaa tccgcaccag cagcgcgggc agccacagca
     2221 ccttcaccct gagcaaagac caaaacgcga acggtcttac ccaagccatg agggagcaca
     2281 accacatcac gcaactgttg gtcagattgg cgcgaatcga gcgtagtgcg catgtgaact
     2341 tcaatcgtgg aatcaaactt ggtaatgctg gtttctttcg ccagagctac cgcttctttc
     2401 gcagcgtaga tcttttcaat atcaactttg gcgagagccg ccgtatattt ttttccgtgt
     2461 ttagccattt tatcctccgt ggtacaagcg gacagcccga agccgtcctc ccacaattaa
     2521 ttagtcaact atcgtaatac ccatcgagcg ggcggtgcct tcgatctgca acatagcacc
     2581 ttcaaggctg atcgcattga gatctttcat cttcaattca gcgatctctt taacctgagc
     2641 gcgggtcacc ttaccgacct tttccttgtt cggaacgccg gaacccttgt caatcttcgc
     2701 agccttgcgc aacaacaccg cagcaggcga cgtgcgaagc acgaaggtga aagaaccatc
     2761 ggtatagatt gtgatctcag cggggagaac ttcacccggg cggttggaag tacgagcgtt
     2821 gtattcctta caaaacgcca tgatgttgat accatgactt gccaacgcag gaccaaccgg
     2881 aggagccggg tttgccttgc cagcttcaag ctggagacgt acgattgctt ttagtttctt
     2941 tgccatttca tttactcctg tggttttagc gggtcattct gggagaccct cccaccgcat
     3001 caggctttgt tttagcctgc tactgtctat ttagttttat ctacactgtt aagacttctc
     3061 aacctgcaag aagtccaatt ctactggcgt ttcacggccg aagaagctca ccatcacacg
     3121 aaccttggtg cgttccatat cgatctcaga aaccacacca cggaagtcgt tgaacggtcc
     3181 atcgataatg cggacgcgtt cgccctgttt gaatgtgacc ttcaccgtgg gcgactcagc
     3241 ttccatacgc ttgacgatct gcgccacttc ttctggacga agcggggttg ggctgttgcc
     3301 cattcccacg aaaccggtca caccgggggt gttgcgcacc acataccagg attcttcagt
     3361 aagaatgagg ttcaccagaa cataaccggg gaagatgtgg cgttccaccg tccggcgttt
     3421 gccatctttc acttcgatct cttcctgtgt cggaatgacc acatcaaaga tcatatcctt
     3481 catccccatc gtttcaatac gttgctcaag gttatgacgc actttattct cataccctga
     3541 ataacagtgg atcacaaacc aagcaggacc ctcaacgatt gtttcgacag gaggcaggtt
     3601 cgaaggaatc ttctcgactg caggagcagg ctcaggcgtc acgggtgaag cggaagaatc
     3661 cgaaggcaca gattgtgctt cggactgttc aatcggttcc tgttcaaact gctcttgcgg
     3721 ttccggtaaa tccattacgt cctcaattca atttacacac caagcatcag gttgaggagt
     3781 tctttggcaa gcgcatccac gcttgcgaga aaaagtccca taacaaccat gacgattaag
     3841 acgagaatcg tgaggcgacg cgcttcgggc caagtgggcc agctaacttt gcgtaattcg
     3901 cctgtcgttt cgcgaaaata gcgctgaacg gcgttctctt ttttcttgac ttgctttgct
     3961 ttctcagcca aggtattgct ccttcaggtt tttacggcta aaacgccgcg gggcgcggcg
     4021 ttgtgcctga ggcaggccag gtaggacttg aacccacaac cgctcgtttt ggagacgaga
     4081 gctctgccaa ttgagccact ggcctaggat gtaggggcgg ttccccgccc ctactgaatt
     4141 actttgtttc gcgatgtact gtgtgcttct tgcagcggtc gcagtacttc ttcaattcca
     4201 tgcggttcgg atcgttgcgg cgattctttt cagtggtgta gttgcgttcc ttgcagtcgt
     4261 tgcactgaag ggtgatcacc ggacgaacat ctttcttctt cgaagccatt ttactacctt
     4321 actttcagtt tcttacgcaa tgatcttggt gacgacaccc gcaccgacgg tgagaccgcc
     4381 ttcacg
//
LOCUS       DAOQJE010000186         4362 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_12085_length_4362_cov_5.224286, whole genome shotgun sequence.
ACCESSION   DAOQJE010000186 DAOQJE010000000
VERSION     DAOQJE010000186.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 4362)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4362)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4362
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12085_length_4362_cov_5.224286"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            83..610
                     /locus_tag="PLT08_06360"
     CDS             83..610
                     /locus_tag="PLT08_06360"
                     /inference="COORDINATES: protein motif:HMM:NF023096.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3267 domain-containing protein"
                     /protein_id="HNE04119.1"
                     /translation="MNKRDLSVSMERANIIVLFIGIPVVILQFILFDQLHPSPAETGE
                     WNFLLLLLVIVMGVILHEIVHGVTWAIAGRKPWTSIKFGVQAKTLTPYCHITEPLEIN
                     AYRIGAVMPGLVVGVLPYIYSLMSGNMNWLWFSLVHTSAAGGDWLVLWLIRHVKAGSL
                     VEDHPSQAGCYVLEG"
     gene            613..1572
                     /locus_tag="PLT08_06365"
     CDS             613..1572
                     /locus_tag="PLT08_06365"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HNE04120.1"
                     /translation="MKKRIQKIAGGFFIVILVILLVGPFLIPVPPLENTVTAESLADE
                     DSQFIEVNGLNVHYKTYGTGEPIFILLHGFGASLFSWHEVTAPLAEYGTVIAYDRPAF
                     GLTERPLEWEGESPYSQDAQVELVIGLMDALGIEKATLVGNSAGGTVSMLTALKYPER
                     IESLILVDPAVYAGGGAPAWIRPLLGTPQMRHVGPLISRQLQAQGVEFIKTAWHDPSK
                     IPQSTLEGYQKPLQVENWDKALWELTLASRESGLAERVSEFNLPILVITGDDDRIVPT
                     EQSLRLAEELPNAELAVIPQCGHVPHEECPDDFMQAVKGFLGK"
     gene            1575..2840
                     /locus_tag="PLT08_06370"
     CDS             1575..2840
                     /locus_tag="PLT08_06370"
                     /inference="COORDINATES: protein motif:HMM:NF012296.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HNE04121.1"
                     /translation="MAQQLSPETGRATLKALFHERSPLGPLKVMAKHVGRFFQIPIPG
                     FSPYVVFGAEAARKVLITDRKKLLWRNTDPVTDLLRRGVLVTDGEEHDHYRSLMETPF
                     HPSQLSKYSEMMIQQVDRVISTWQDGQMVDMLVEGRKIALLIIMQTLFNKDAWNDIPK
                     IWKPILKTIQYVSPGMWILWRKIPRLGYGKSLKVIDDYMYAIIDERRKEERKTNDFLQ
                     HLIDAGLSDDIIRDQMLTMLIAGHDTSTALFAWTFALLGQHPEIHANVAQEVDRAEGK
                     YQLLDNVIKESLRLYPPIHIGNRRVAEEMEFAEGNIPAGERMFYSIYLTHRDPEVWEN
                     AEQFCPERFAHGRKTPPMSYIPFGGGPRACIGAAFGQAEARIVLAHLLKNFTFEFTGH
                     TIHAHMGATLEPRPGVKMKVKRRTVASVQ"
     gene            2906..3700
                     /locus_tag="PLT08_06375"
     CDS             2906..3700
                     /locus_tag="PLT08_06375"
                     /inference="COORDINATES: protein motif:HMM:TIGR03462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lycopene cyclase domain-containing protein"
                     /protein_id="HNE04122.1"
                     /translation="MTYFGFLLRFLVIPILVFLVITWWDNKNNRPTFGFNNGKAVWVA
                     ILIHIVLAVVYTTPWDNYLVATGVWYYNPNLVTGIVIGYVPIEEYTFFVLETILAGLW
                     WWFLARRIPEPTNTFKPNKTGRIISFSVLFIFWVIFTYLFFFGGVELTYLSIILFWAL
                     PAIFPQMLYGADILWHYRKLVFITIFVPGLYLSLMDIIALTDTTWSISKEQTTGILFF
                     NILPLEEVLFFFITNVLIGFGMTLLLSKLGTQRFEDWKSNNYKGLP"
     gene            3697..4071
                     /locus_tag="PLT08_06380"
     CDS             3697..4071
                     /locus_tag="PLT08_06380"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PPOX class F420-dependent oxidoreductase"
                     /protein_id="HNE04123.1"
                     /translation="MKNIEQFKKQQYINLETFRKNGGGVKTPVWFAQDGETLRIWTQA
                     DAGKTKRIRRDGKVRIAPSTASGEPIGEWIDANATVLNTPDEINYTKDLFRKKYGVMF
                     NVFGFLGKVRGSSFVTIKVNLN"
     gene            4154..>4362
                     /locus_tag="PLT08_06385"
     CDS             4154..>4362
                     /locus_tag="PLT08_06385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE04124.1"
                     /translation="MINQIEQNILNLLQHLYDELGWFGVTIIMIVENSTGIAPSEVVL
                     ALAGWMLIDAHSLPFSTVFLGGLFAA"
BASE COUNT         1131 a         1023 c         1041 g         1167 t
ORIGIN      
        1 atccatctga accaatcgta gatcgagacg tggtaaaaga gcgcacgttg agcgcatata
       61 gtgtttcaaa ggaaaaaata cgatgaacaa acgtgatcta tctgtttcga tggaacgcgc
      121 aaacatcatt gtgcttttca tcgggattcc tgtagtgatt ctacaattca tcctctttga
      181 tcaattacac ccatcgcccg ctgaaactgg tgaatggaat ttcttgttgt tgctcttggt
      241 cattgttatg ggtgtgatac ttcatgagat cgtgcatggg gttacctggg caattgcagg
      301 gcgtaagcct tggacttcaa ttaagttcgg ggttcaagct aaaacgctta caccgtattg
      361 ccatataact gagccgcttg agattaatgc gtaccgtatt ggtgcagtca tgccggggtt
      421 agtcgttggg gttttgcctt acatctatag tcttatgagt ggaaatatga actggctatg
      481 gttcagcctc gtccacacct ccgccgctgg tggagactgg cttgtgctct ggctcatccg
      541 ccacgtaaag gcgggttctc tggtagaaga tcatcccagc caggctgggt gttacgtcct
      601 cgaaggataa atatgaaaaa acgaatccaa aaaattgcgg gtggattttt tatcgtaatc
      661 cttgttatct tattggttgg tccgttcctc atcccagtac caccgttgga aaacacagtg
      721 accgccgaaa gtttggcaga tgaagatagt caattcatcg aagtcaatgg tttgaatgtg
      781 cattacaaga cttatggaac gggcgagccc attttcattt tgctgcacgg ctttggcgcg
      841 agtttgtttt cgtggcacga agtgacagct ccacttgcgg agtatggcac ggtcattgca
      901 tatgaccgcc ctgcctttgg tctcactgag cgtccgctag aatgggaagg tgaaagccca
      961 tacagtcagg atgcgcaagt agaactggtc attggtttga tggatgcact tggcattgag
     1021 aaggcaacct tggttgggaa ttctgcgggc ggaacagtct ctatgttgac ggcgttgaaa
     1081 taccccgagc gaattgaaag cctcattctg gttgacccag ccgtgtacgc aggtggaggc
     1141 gcgcccgcat ggattcgtcc actgctgggc acaccacaga tgcgtcatgt cggtccgttg
     1201 atctcacgtc aacttcaggc gcaaggcgtg gaattcatca agactgcctg gcatgaccca
     1261 agtaagattc cacagtccac gcttgagggg tatcaaaaac cgttacaagt ggaaaactgg
     1321 gacaaagcct tatgggagtt gacactcgcc agccgcgaaa gcggattggc agaacgggtt
     1381 agcgagttca atttgcccat cctcgttatt actggcgatg atgatcgcat cgttccaacg
     1441 gaacagtctt tacgacttgc agaagagcta cccaatgcag aactggctgt catccctcaa
     1501 tgcgggcatg tgccgcacga agaatgccca gacgatttca tgcaagcagt gaaaggcttt
     1561 cttggaaagt aatcatggca cagcaacttt ctcctgaaac cggtcgcgcc actttgaaag
     1621 cgctcttcca cgaacgctcg ccattaggtc cattgaaagt aatggcaaag catgtggggc
     1681 gcttctttca gattcccatc cccgggttca gtccatatgt cgtcttcgga gcggaagcgg
     1741 cacgcaaggt attaatcact gatcggaaga agttgctatg gcgcaacacc gacccagtga
     1801 ccgacctgct gcggcgcggc gtactggtga ccgacggcga agagcatgac cactatcgaa
     1861 gtctgatgga aacgccattc catccctcac agttatcgaa atattccgag atgatgattc
     1921 aacaagtgga tcgagttata tccacctggc aagatggtca aatggtagat atgttagtgg
     1981 aagggcgaaa gattgctctg ctcatcatca tgcagacctt gttcaacaaa gatgcctgga
     2041 atgacattcc gaagatctgg aaaccaatcc tcaaaaccat tcaatacgtc tcaccgggaa
     2101 tgtggatttt atggcgaaag attccgcgtc ttgggtacgg gaagtcgttg aaggtgatcg
     2161 atgattatat gtatgcgatc atcgatgaaa gaaggaaaga ggaaagaaaa acaaatgatt
     2221 ttctgcaaca tttgatcgat gccgggttga gtgatgacat catccgcgac caaatgctga
     2281 ccatgcttat tgccgggcat gatacctcca ccgccttatt tgcatggacc tttgccttgc
     2341 ttgggcagca tcctgagatc cacgcaaatg tggcgcagga agttgaccgc gcagaaggca
     2401 aatatcaact attagataac gtcatcaaag aatccttaag gctgtatcca cccattcata
     2461 ttggtaaccg ccgtgtggca gaagagatgg aatttgcaga aggcaatatc ccggctgggg
     2521 agcgtatgtt ctactccatt tacctcaccc atcgcgaccc cgaggtctgg gaaaatgctg
     2581 agcaattttg ccccgagcgt ttcgcccatg gacggaaaac tccgcctatg agttacattc
     2641 cgtttggcgg cggtccacgg gcttgcattg gcgcggcatt cggtcaggca gaggcgcgga
     2701 tcgtcctcgc tcacttgctc aagaacttca cattcgaatt cactggtcat actattcacg
     2761 cgcacatggg tgccacactg gaacccagac cgggcgtaaa gatgaaagtg aagagaagga
     2821 ctgtagccag tgtgcagtga agtatgactt atacaaacga ctatctgact aacaaactat
     2881 tagactaagt gactaacaaa ctacaatgac atactttggt tttctcttac gctttctcgt
     2941 cattcccatc cttgtttttc tcgtcattac atggtgggat aataaaaaca atcgccccac
     3001 atttggtttt aacaatggta aagccgtatg ggtcgcgatt ctgattcaca tcgtccttgc
     3061 cgtggtgtat accacgccgt gggataacta tctagttgcc accggcgttt ggtattacaa
     3121 ccccaatctt gtgacaggca ttgtaattgg ttacgtgcct attgaagagt acaccttctt
     3181 tgtgctagaa accatcctcg ccgggttatg gtggtggttc cttgcacgtc gtatccctga
     3241 accgacgaac acattcaagc ctaacaaaac cgggcgcatc atctcattca gtgttctgtt
     3301 tattttctgg gtcatcttca cctatctgtt cttcttcggc ggcgtggaat tgacctatct
     3361 ctcaattatt ctgttctggg cgcttcctgc catcttccca caaatgctgt atggcgccga
     3421 tatcctctgg cattaccgca agttggtttt catcacgata tttgtcccag gcttatatct
     3481 ttctctaatg gatattatcg ctctcacaga caccacctgg tctatttcga aggaacagac
     3541 cacaggtatt ctcttcttta atattctgcc attagaagaa gttttattct ttttcatcac
     3601 caacgtcctc attggctttg gcatgacatt gcttctttca aaacttggca ctcagcgctt
     3661 tgaggattgg aaatcaaaca actacaaagg tctaccatga agaacattga acaattcaaa
     3721 aaacaacaat acataaacct tgagacgttt cgcaaaaatg gcggaggggt caaaactcca
     3781 gtttggttcg cacaggatgg agaaacgctt cgtatctgga cccaagcgga tgccggcaaa
     3841 accaagcgca tccgccgtga cggcaaagtg cgcatcgcac catccacagc ttcaggcgaa
     3901 cctattggcg aatggattga tgctaatgcc acagttctta acacaccgga tgaaataaac
     3961 tacacaaaag acctttttcg caaaaagtac ggagtaatgt tcaatgtatt tggcttttta
     4021 ggaaaagtcc gcggctcaag cttcgttacg atcaaagtaa atcttaacta attttgctct
     4081 cattgggatt aaaccatggc tcatcttttt tatgtaagat agaagccagt ttagatctag
     4141 agacaagaat agaatgatca atcaaataga gcagaatatc ttaaatttac ttcaacatct
     4201 atatgatgag ctaggttggt ttggcgtcac catcatcatg attgttgaaa actcaactgg
     4261 catcgcccct agtgaggtcg tgcttgccct tgctggctgg atgttgattg atgcacatag
     4321 tttgcctttt tctaccgtgt ttcttggagg gcttttcgcc gc
//
LOCUS       DAOQJE010000187         4361 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_12093_length_4361_cov_6.859266, whole genome shotgun sequence.
ACCESSION   DAOQJE010000187 DAOQJE010000000
VERSION     DAOQJE010000187.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 4361)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4361)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4361
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12093_length_4361_cov_6.859266"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            13..129
                     /locus_tag="PLT08_06390"
     CDS             13..129
                     /locus_tag="PLT08_06390"
                     /inference="COORDINATES: protein motif:HMM:NF013854.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047429 - nucleoside triphosphate
                     diphosphatase activity [Evidence IEA];
                     GO_process: GO:0009143 - nucleoside triphosphate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="non-canonical purine NTP pyrophosphatase"
                     /protein_id="HNE04125.1"
                     /translation="MPELGKTMSELDMSEKNRLSHRARAVIAAMPILKRIFA"
     gene            complement(212..988)
                     /locus_tag="PLT08_06395"
     CDS             complement(212..988)
                     /locus_tag="PLT08_06395"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HNE04126.1"
                     /translation="MPFVQGIHYSFQQSKETNPSRPPLIFIHGAGGSFLSFHPYLRHI
                     QGETIYALDLPGHGKSVGKGRQSIEEYADDIARFMDDLQIASAVMVGLSMGSGIALSF
                     ALKYPHKVSALVVMGGGAKLRVAQSTLENVGKPETFEATVETINRACFSTYASNELVE
                     LSKKSMLSTGPDILLSDYLACNEFDVTTQLNQIQIPTLLLCGMEDAMTPPKYSQYLRD
                     RLPNAQLHLLEKTGHMVTLEQPELVAKLLKQFLDELPPLS"
     gene            complement(992..2749)
                     /locus_tag="PLT08_06400"
     CDS             complement(992..2749)
                     /locus_tag="PLT08_06400"
                     /inference="COORDINATES: protein motif:HMM:NF024883.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing sensor histidine kinase"
                     /protein_id="HNE04127.1"
                     /translation="MKRFETPYMADWFAISLRWIMLVGLIVSLGLGQRLDASASWPLG
                     LMIVWNLGMTALAGLNIRTQYHRRLNIAMDLILTAAFFWAQGGVRGPAFWAGLLPILT
                     GSIYFELIGAVVAAILFSGFVIYTGTRVDGNITLAFLVAAILSLLGVLFGFLGRRLTD
                     HMRKNRALWVDAEEKKRAIQNERMRAIYELTSTLSTTLSYKRVLDSALDMSASALNPN
                     LDPEQTTSDPLVGAVMLFNAGKLRIGSARRFTNADMRITFDGAEGVLKKALDEGEPVT
                     FKDIGYDQELGRVIALRNCTGGYVFPLRSGFNVYGVLLFAHPEPDFFNTERTNLLDII
                     GRQAVIAIQNARLYQDLVEEKERLADVYEEARKKLARDLHDGPTQSVAAMAMRLNIAR
                     RMLTKDTKMANEELVKLEELAHRTTKEIRHMLFTLRPLILESQGLGAAIQSMADKMME
                     TFSQRVVVEIDDRAVQQLEMGKQGVIFYIIEEAVNNARKHAAAEAITVRLRQVDTGIV
                     LLEIQDNGAGFDVKAITQNYDKRSSSSLGMINLQERAELVNGLLNIDSAPGKGTNVQV
                     YIPLTEEAADRLHHARRSA"
     gene            complement(2828..3556)
                     /locus_tag="PLT08_06405"
     CDS             complement(2828..3556)
                     /locus_tag="PLT08_06405"
                     /inference="COORDINATES: protein motif:HMM:NF012888.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008146 - sulfotransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase domain-containing protein"
                     /protein_id="HNE04128.1"
                     /translation="MIVLSVGMPRAGSGWHYNLIHDLMKTTGCADAQDIRKKYGLQKI
                     LTEVNCNIGVLSPRRLAMVTVPAVLGNTFVIKAHASPSSASRFLASLGMLRITYIYRD
                     PRDAMLSAFDYGQRALAKGRPNAFSHLSDFEKSVDFMMEYVHIWEKWMKEKNVLIARY
                     EDLLTNYEVESAKLVDYLKLDANKPEVRAVIEQYRPGANDGQQGLHFYKGKIGRFRDA
                     YTSEQQNVLLEKLSPYLERMNYEK"
     gene            complement(3553..>4361)
                     /locus_tag="PLT08_06410"
     CDS             complement(3553..>4361)
                     /locus_tag="PLT08_06410"
                     /inference="COORDINATES: protein motif:HMM:NF016800.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HNE04129.1"
                     /translation="VLTKYRVTRLSWLEPALLAVTTLVLLATYSRGGLLIILGTSGLL
                     MLLFGRDILKGMWAWFISGFRGRISDFALRIGMVAVLVGIVAGAVLFLGQKNYFRRLW
                     EINAESLSEYVVDINAGARGAYSVGALGAFSEYPLTGVGLGGSGFYIYKNLPDWALTT
                     VPEIAKQLNPSNKLYPNPKNLYVRLLAETGLFGFVLFFAFLFNVLGDMLSLLKRSEPW
                     ARFAAVAGVFAWVAIALYNFTQDSLTTPNIWIVPGILVGLATHQLNKEVS"
BASE COUNT         1220 a         1114 c         1001 g         1026 t
ORIGIN      
        1 cctatctttt tcatgccgga actcggcaag acaatgtctg aactggacat gagtgagaag
       61 aataggctca gtcatcgggc gagggcagtg attgcggcaa tgccaatctt gaaaagaatt
      121 tttgcataaa aaaataaata gccagtcaca ccacaagtga ctggctattt ttagattaag
      181 aaaaaactaa atgggcagaa tcagctaccc gttaggaaag aggcgggagc tcatccaaaa
      241 actgtttcaa taactttgca actaactccg gctgttcaag tgtcaccata tgtccggttt
      301 tttccagcaa gtggagttgg gcattaggaa gacggtctct taagtattgt gaatattttg
      361 gaggggtcat ggcatcttcc ataccgcaaa gaagcaatgt tggaatttgg atttggttca
      421 gttgggtagt gacatcaaac tcattacaag cgagataatc acttaataaa atatctggac
      481 cagtgctcag catactcttt tttgaaagtt caacaagttc attagaagca tatgtactaa
      541 aacacgcacg gttaatggtt tcaacagtgg cttcaaaggt ttcaggctta cccacattct
      601 caagcgtgga ttgtgcaact cttaattttg ctccgccacc catcacaacc aatgcactta
      661 ctttatgcgg atatttcagt gcaaaagata aagcaatgcc acttcccatt gaaagcccaa
      721 ccatgactgc tgatgcaatc tgaaggtcat ccataaaacg cgcaatatca tcagcatact
      781 cttcaataga ctgtctacct ttgcccacag acttgccatg ccctggcaag tctaacgcat
      841 agatagtctc tccttgtata tgccgtagat atgggtgaaa ggataaaaaa ctgccgcccg
      901 caccgtgtat aaaaatcaac ggtgggcggc tgggatttgt ttctttggac tgttggaacg
      961 agtagtgaat gccttgcacg aacggcatgg cttatgcaga cctgcgagca tggtgtaagc
     1021 gatcggcagc ttcttcggta agcggaatat agacctgcac gttggtgccc ttgcccggcg
     1081 cggagtctat gtttagcaag ccgttcacca attcagcacg ctcttgcagg ttgatcatac
     1141 ccaagctgct gctggaacgc ttgtcataat tttgcgtaat ggctttcacg tcaaagcctg
     1201 cgccgttgtc ttgaatttct aaaagcacaa tccctgtatc cacttggcgc aagcggacgg
     1261 taatcgcttc ggcagcagca tgtttgcgag cattgttaac agcctcttcg atgatataga
     1321 aaatgacgcc ttgtttgccc atctccaatt gctgaacagc acggtcatcg atctcaacca
     1381 ccacacgttg tgagaaagtc tccatcattt tatctgccat ggattgaatg gcagccccca
     1441 aaccttgcga ctcaagaatc aacgggcgca gggtaaagag catatggcga atttctttgg
     1501 tggttcgatg tgcaagttct tccaatttga cgagttcttc gttcgccatt ttggtgtctt
     1561 tcgttaacat acggcgagcg atgttcaaac gcattgccat ggcagctaca gactgggtag
     1621 gtccatcgtg caggtctctt gctagctttt tacgagcctc ttcgtacaca tccgcgaggc
     1681 gctctttttc ttccaccagg tcctgataaa ggcgggcatt ctgaatggcg atcactgcct
     1741 gacgcccaat gatatccaaa agattagtcc gttcggtatt aaaaaaatca ggttcagggt
     1801 gagcaaacaa aagcacacca tatacgttga aaccgcttcg taaagggaaa acataaccgc
     1861 ccgtgcaatt gcgcaaggca atgacacgtc ccagttcttg atcgtaacca atatctttga
     1921 aggtgacagg ctccccttcg tcgagcgctt ttttcaaaac gccctctgca ccatcaaaag
     1981 taatgcgcat atcagcgttg gtgaaacgac gagcggagcc aatgcgtaat ttacccgcat
     2041 taaaaagcat aacagcgccc actaatgggt cgctggtcgt ttgttctggg tcaagattgg
     2101 gattcaatgc agaagcgctc atatcgagcg ccgagtcaag cacacgttta tagctaaggg
     2161 tcgtggaaag cgtggaagtc aattcgtaga tcgcacgcat acgttcattt tgaatggcac
     2221 gtttcttttc ttcggcatca acccacaaag cccgattctt gcgcatatgg tcagttagcc
     2281 ggcgacctag aaacccaaag agtaccccta aaaggctcag aatcgcagct acgagaaatg
     2341 caagggtaat attcccatcc acccgggtgc cggtgtagat aacaaagcct gagaacaaaa
     2401 tggcagccac cactgcgcca attagctcaa agtagatgga accggtcaaa atagggagca
     2461 atcctgccca aaaagcaggt ccccgaacgc caccttgcgc ccagaagaat gccgctgtaa
     2521 ggatcaaatc catcgcaatg ttgaggcggc gatgatattg tgtacggatg tttaagccag
     2581 ctagagccgt catccccaag ttccaaacaa tcatgagccc cagaggccag ctggcactcg
     2641 catctaatct ctgtccaagt ccaagagaaa cgataagacc cactagcatg atccagcgca
     2701 gtgagatggc aaaccagtct gccatgtagg gagtttcaaa tcgtttcatc gctgccctat
     2761 gataacgaaa aagggatgtt tcgacaacat ccccgacgtt acagtcagat tacacctagt
     2821 gcaaatatta cttttcgtag ttcatacgct ccaaatacgg gcttaatttc tctaaaagca
     2881 cgttttgctg ctcgcttgta tacgcatcac ggaaacgccc gatcttgccc ttgtaaaaat
     2941 gcaagccttg ctgcccatca tttgcgccag ggcgatattg ttcgatcacc gcgcggacct
     3001 caggcttgtt cgcatccaac ttgagataat ccaccagttt ggcagattca acttcatagt
     3061 tggtaagtag gtcttcgtaa cgcgcgatca acacattttt ctctttcatc cacttttccc
     3121 aaatgtggac atattccatc ataaaatcca cgcttttctc aaaatcggaa aggtgtgaaa
     3181 aagcgttggg acgtcccttg gcaagcgcgc gctgaccgta gtcgaaggca gagagcatgg
     3241 catcacgtgg atcgcggtag atgtaggtaa tacgcagcat acccagtgaa gccaagaagc
     3301 gggaagccga tgaaggactc gcatgcgcct tgatcacaaa ggtattgccc agtacggctg
     3361 gcacagtcac catcgccaaa cggcgcggag aaagcacacc aatattgcaa tttacctctg
     3421 tgaggatctt ttgcaaccca tacttcttac ggatatcctg cgcatcggca cagcccgtgg
     3481 ttttcatcag gtcgtgaatg aggttatagt gccagcccga cccagcgcgc ggcatcccaa
     3541 cagaaagaac gatcatgaaa cttccttgtt caattgatgt gtagcaagcc ccaccaagat
     3601 ccccggcacg atccagatat tcggggtggt aagcgaatcc tgggtgaaat tatacagggc
     3661 gatagccacc cacgcaaata caccagccac cgccgcaaag cgcgcccacg gctcgctccg
     3721 tttgagcagg gagagcatat ctccaagcac attgaagaga aatgcaaaaa agagaacgaa
     3781 gccaaataac ccagtttccg caagcaagcg cacatataaa ttcttggggt tcgggtacag
     3841 tttattcgat ggattcaact gcttcgcgat ttccggaacg gtggtcagcg cccaatcagg
     3901 cagattttta tagatgtaaa acccgcttcc tcccaagccg acgcctgtta acgggtattc
     3961 tgaaaacgct cccaacgccc caaccgagta cgctccgcgc gccccggcgt tgatgtccac
     4021 aacatactcg ctcaaactct cggcgttaat ttcccacaag cggcggaagt aattcttctg
     4081 cccgaggaac aagaccgcgc cagctacaat accaaccaac accgcaacca taccaatacg
     4141 caatgcaaag tcagagatac gtccacgaaa gccgctgatg aaccaagccc acataccttt
     4201 aagaatatcg cgaccaaaga gcaacataag caagcccgat gtgccaagga tgatgagcaa
     4261 gccgccgcgg gaataggtag ccaaaagcac caatgttgta actgctagca acgcaggctc
     4321 cagccacgat aggcgtgtga cgcggtactt cgtcaaaaca g
//
LOCUS       DAOQJE010000188         4358 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_12112_length_4358_cov_5.260748, whole genome shotgun sequence.
ACCESSION   DAOQJE010000188 DAOQJE010000000
VERSION     DAOQJE010000188.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 4358)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4358)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4358
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12112_length_4358_cov_5.260748"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..755)
                     /gene="yqeB"
                     /locus_tag="PLT08_06415"
     CDS             complement(<1..755)
                     /gene="yqeB"
                     /locus_tag="PLT08_06415"
                     /inference="COORDINATES: protein motif:HMM:TIGR03309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenium-dependent molybdenum cofactor
                     biosynthesis protein YqeB"
                     /protein_id="HNE04130.1"
                     /translation="MNPLILIRGGGDLASGVALRLHRVGFQVAILELDKPLAVRRTVS
                     FSEAVYEGSQTVESVTARLISADQFQVTLEAGEIPVLIDPHANILNNQFLTSPTSTFV
                     VDARLMKTEPEPLPERVALHIGLGPGFTAGANCHAVIETMRGHALGRVYWDGQTAPDS
                     RQPDGDTRRVLRAPAAGTLQPNKAIGAFCKEGETLAFVSGKPVISPFDGILRGLIHPK
                     VELTEGMKIGDVDARNEPSMVRFVSDKALSVAGG"
     gene            complement(752..1468)
                     /locus_tag="PLT08_06420"
     CDS             complement(752..1468)
                     /locus_tag="PLT08_06420"
                     /inference="COORDINATES: protein motif:HMM:NF018766.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1361 domain-containing protein"
                     /protein_id="HNE04131.1"
                     /translation="MFQKIYNKLKPFRFRLTILGLLAVASLISIALFRFRTRISGTME
                     YDFLVWNLFLAWLPLGMSYIASSFTNKRPFVIALIVPVAAVLWLLFFPNAPYILTDLF
                     HLRHPREIVPIWYDTLLINWFAWTGVLLGVFSLFMMHDIVRRVFGRFIGWLFVLTVST
                     LCGVGIYIGRFLRWNSWDVILHPYERMREMLYYVLHPSMQSILFISVFSALFVFIYIT
                     TYTFGLLFQEQAQPISQDIS"
     gene            complement(1535..2290)
                     /locus_tag="PLT08_06425"
     CDS             complement(1535..2290)
                     /locus_tag="PLT08_06425"
                     /inference="COORDINATES: protein motif:HMM:NF018766.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1361 domain-containing protein"
                     /protein_id="HNE04132.1"
                     /translation="MTFNRAKNFLFRNRHNLAVFVLLNLACVACIVFVAARVVYTDSS
                     RHTGLIWNLFLAWIPFILAYFAHALSWKKVWVYLAIPFVAFLWLLFFPNAPYMLTDLQ
                     DLARGTGYEAPLWYDVIIVVWCSWTGTLLGVISLYLMQDIIARTFHRWLGWVFVFAIS
                     GLSSFGIYIGRFVRLNSWDILQNPAETAMEILGIVIDPSKRLAAFTLAYTFFFLFIYL
                     LLYSFSHMLQEQVIRAQSSPEQLASTQPTQPVH"
     gene            complement(2287..2781)
                     /locus_tag="PLT08_06430"
     CDS             complement(2287..2781)
                     /locus_tag="PLT08_06430"
                     /inference="COORDINATES: protein motif:HMM:NF017015.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HNE04133.1"
                     /translation="MNKEYIQTLYRYNQWSNARILDTAAKLSPAQFLATSEHPHRYLR
                     GVLTHTLFAEWIWHKRWTGESPTTRLKPEDFPTFEALRTRWAEEESQLMQFVAQVTDE
                     KLNAPFTYLATDGTKYENILWESMAHVVNHGMQHRSEAAAILTELGVSPGDLDFILFT
                     RKKL"
     gene            complement(2833..3708)
                     /locus_tag="PLT08_06435"
     CDS             complement(2833..3708)
                     /locus_tag="PLT08_06435"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HNE04134.1"
                     /translation="MPFPYLDETLLLNAETRKDLPGSFIALPSGVTHYEEGGDPKGQP
                     VVLVHGFSVPYFIFEPTFVFLCQQGFRVLRYDLLGRGFSDRPNTRYNATLFVQQLKEL
                     LDALNYKNICLAGLSMGGAVTSSFIDAYPDYVSKYVLIDPSGARAVSLSPFLKAVRMP
                     GFGELAIGLFGSESMLKGIASDLFSPELVREFQEKYKVQMQYQGFKRAILSTMRNGML
                     DSFIETYKRVGKLKKPTLLFWGKQDTTTPFEHSADICAVMPHAEFYAFDSCGHIPHYE
                     RASEVNRILLEFLRK"
     gene            complement(3769..>4358)
                     /locus_tag="PLT08_06440"
     CDS             complement(3769..>4358)
                     /locus_tag="PLT08_06440"
                     /inference="COORDINATES: protein motif:HMM:NF014848.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE04135.1"
                     /translation="VTEQTAEAGLSAPPAIAGALLSGLLSDTLILTSPTTTDRDRVAA
                     ERLARWAFVGGSPLQGETIETYGKAVLSAGAGLSNRKPEEVVSTDIKPFEGGGFKFAV
                     AQAEVTDIMQLSEHLAPLTKALDDLRDKRGLDFALLLVTDVVRGTSRLIVTSSAPPIL
                     NDLPYPPLPDGTRDAPGVVSRKKQLLPAVLGLLER"
BASE COUNT         1200 a         1032 c         1116 g         1010 t
ORIGIN      
        1 ccgcctgcaa cggacaaggc tttatctgaa acaaaacgga ccatgcttgg ctcattgcgt
       61 gcatccacat cgccgatctt catgccttcg gtcaactcca ctttggggtg aatgagtccg
      121 cgcaagatgc catcgaacgg gctgataacc ggcttgccgc ttacaaaggc gagggtttct
      181 ccttctttgc aaaacgcgcc aatggcttta ttgggttgca acgtccctgc ggcaggggcg
      241 cgtagtacac ggcgggtatc accatcgggt tggcggctat cgggcgcggt ttgcccatcc
      301 caatacactc tgccgagggc atgtccgcgc atggtttcga tcacagcgtg gcaattggct
      361 ccggctgtaa acccaggtcc cagcccgata tgaagcgcca cacgctctgg caatggctct
      421 ggctccgttt tcatcaaacg cgcatccaca acgaatgtgg aagttggact cgtcagaaat
      481 tgattgttga ggatgttggc atgtgggtcg atcaacacag gaatttcacc cgcctctaac
      541 gtgacctgaa actggtctgc cgaaataagc cttgcggtta cgctttcaac cgtttgtgag
      601 ccttcataaa ccgcctctga aaatgagacc gttctacgaa ctgccaaggg tttatccagt
      661 tcgagaatgg caacctgaaa accaacgcgg tgcaagcgga gcgccacgcc tgaagcgagg
      721 tcaccgccac cgcgaatgag aatgagtgga ttcatgaaat atcctgtgaa atgggttggg
      781 cttgttcttg aaacaataaa ccgaatgtat aagtggtaat gtagatgaaa acaaacaaag
      841 ccgagaagac actaataaaa agaatcgatt gcatgcttgg atgcaagaca tagtatagca
      901 tctcgcgcat gcgttcataa ggatgcagaa tgacatccca tgaattccag cgcaagaagc
      961 gcccgatgta aatgcccacc ccgcaaagtg tgctgaccgt gagcacgaat aaccaaccga
     1021 tgaaacgccc aaacacacgc cgtacgatgt cgtgcatcat gaaaagcgaa aagacaccca
     1081 acaagactcc ggtccatgcg aaccagttga tgagtaaggt gtcgtaccaa atgggcacaa
     1141 tctcacgcgg atggcgcaag tggaataggt ctgtcagaat gtagggagca ttcgggaaga
     1201 ataacagcca taaaacagcc gcgacaggga caattaacgc gataacgaag ggacgcttgt
     1261 tcgtaaaact tgaagcgatg tacgacattc ccaaaggcaa ccaagcgagg aacagattcc
     1321 aaaccaggaa atcatattcc atggtgccgc tgatgcgggt gcgaaagcga aataacgcaa
     1381 ttgagataag gctggctact gctaatagcc ccaaaatagt cagacgaaaa cgaaatggtt
     1441 ttagtttgtt atatattttt tgaaacatag tatctccggt gtttgaccca ttgtaagcag
     1501 atgggcttcc aattccttgc aaagactagc aggcttaatg tactggttgt gttggctgcg
     1561 tagaagccaa ttgctccgga gagctttgag cacggatcac ctgttcttgc agcatgtgac
     1621 taaatgaata caacaacagg taaatgaaca agaagaaaaa ggtatatgcc aacgtaaagg
     1681 cggcgagacg cttgcttggg tcaatgacga tgccgagaat ttccatggcg gtttcggcgg
     1741 ggttttgcaa aatgtcccat gaattcaggc gcacgaagcg accgatgtaa atgccaaaac
     1801 tgctcagccc tgaaattgca aagacgaaga cccatcccag ccagcggtgg aaggtgcggg
     1861 cgataatgtc ttgcatcaaa tagagcgaga tgacgcccag caaagttcct gtccacgagc
     1921 accagacgac gatgatgaca tcgtaccaaa gcggagcttc ataacccgtg ccgcgagcga
     1981 ggtcttgcag gtcggtcaac atgtatggag cgttggggaa gaacaatagc cacagaaaag
     2041 ccacaaatgg aattgccaga tacacccaaa cttttttcca cgaaagagca tgagcaaaat
     2101 acgcaaggat gaacggaatc catgccagga agagattcca aatcaaaccc gtgtgacgcg
     2161 acgaatcggt atacaccaca cgcgctgcta caaaaacaat acaagccacg catgccaggt
     2221 taagcaagac gaacacagct aggttatgac gattccgaaa aagaaaattc ttggcgcgat
     2281 tgaaggtcat agtttctttc ttgtgaatag gatgaaatct aaatcgccgg gagaaacccc
     2341 aagttcggtg agaatggcag cggcttcact gcgatgttgc atgccatgat tgaccacatg
     2401 tgccatcgac tcccacagaa tattttcata ttttgtgcca tcggtggcta aataggtaaa
     2461 aggcgcgttc agtttttcgt ccgttacttg cgcaacgaat tgcattaatt ggctttcttc
     2521 ctctgcccaa cgtgtacgca atgcttcgaa ggttgggaag tcttccggct tcaagcgcgt
     2581 agtgggtgat tcgcctgtcc agcgtttgtg ccaaatccat tcggcaaaga gggtgtgtgt
     2641 aaggacaccg cgtaaatagc ggtgtggatg ttctgaagtt gctaaaaatt gcgcagggct
     2701 gagtttggcg gcggtgtcga gaatccgcgc attcgaccac tggttatagc ggtacaacgt
     2761 ttgaatatat tctttgttca cggttgctcc tttaatccat cccccaatca ctatgtattt
     2821 gttttgaggg aattacttcc tcaaaaattc taacagaatt ctgtttacct cgcttgcgcg
     2881 ctcgtagtga ggaatgtgcc cacaactgtc aaaggcatag aattctgcat gcggcatcac
     2941 tgcgcatata tcggcactat gctcaaacgg agttgttgta tcttgttttc cccaaaaaag
     3001 aagagtcggt tttttgagtt tgccaactcg tttgtaggtc tcaataaaag agtccaacat
     3061 gccattgcgc atcgtagaaa gaatggcacg cttgaaccct tgatattgca tttggacttt
     3121 atatttttct tggaattcac gcacaagttc tggactgaac aagtctgatg caatgccttt
     3181 aagcatactt tcgcttccga acagaccgat agccagttcg ccaaacccgg gcattctcac
     3241 cgctttgaga aaaggcgaga gcgaaactgc ccttgcgcca gatgggtcaa tgagaacgta
     3301 ttttgaaaca taatcagggt acgcatcaat aaacgacgag gtgaccgctc cacccataga
     3361 tagccctgca agacagatat ttttgtagtt cagtgcatcc aacagttctt tgagttgttg
     3421 aacaaacaga gttgcgttgt atcttgtatt tggacgatct gaaaacccac gcccaagcaa
     3481 gtcatagcgc aagacacgaa agccttgctg acaaagaaat acaaaagttg gttcgaaaat
     3541 aaagtaaggg acagaaaatc catgcaccaa aactaccggc tgaccctttg gatccccgcc
     3601 ttcttcgtag tgtgttactc cactaggcag ggcgatgaac gagcccggca agtctttacg
     3661 ggtttcggcg ttgagtaaaa gggtttcgtc gaggtatggg aagggcatgg tttttcaaaa
     3721 ttatacaaaa taaggcgggt tcaaaacccg ccctatatgg acatttactt atcgttctaa
     3781 taaacctaat acggctggca acaactgttt ctttctcgaa accactccgg gtgcatcgcg
     3841 agtgccgtcg ggtaagggcg ggtagggaag gtcattgaga atgggaggcg cacttgatgt
     3901 aacgatcaag cgactggttc cacgcaccac atcggtgacc agtaacagcg caaagtcgag
     3961 tccgcgtttg tcgcgtaggt cgtcgagggc tttggtgagt ggcgcaaggt gttctgaaag
     4021 ctgcatgatg tcggtcactt cggcttgagc cactgcaaat ttgaatccgc cgccttcgaa
     4081 tggtttgata tctgtgctga cgacttcttc tggcttgcgg ttggaaagcc cagcacctgc
     4141 actaagaacg gcttttccat aggtttcgat ggtttcgcct tgtagggggc tgcctccaac
     4201 aaacgcccag cgtgccaacc gttctgcggc gacgcggtca cggtctgtcg tggtgggaga
     4261 ggttaggatg agcgtatccg acaaaagtcc agaaagaagc gcacccgcaa tggcaggcgg
     4321 ggcagacaag cccgcttcag ccgtctgttc ggtgacga
//
LOCUS       DAOQJE010000189         4354 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_12134_length_4354_cov_6.622936, whole genome shotgun sequence.
ACCESSION   DAOQJE010000189 DAOQJE010000000
VERSION     DAOQJE010000189.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 4354)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4354)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4354
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12134_length_4354_cov_6.622936"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1090
                     /locus_tag="PLT08_06445"
     CDS             <1..1090
                     /locus_tag="PLT08_06445"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNE04136.1"
                     /translation="HRAVFESISDSVIVLDPELRIIELNPAAVEMFKLNIGEVLGKSF
                     QSVFRPWVQLNDKALQTHGYHREIVLENDGEPVRWLHLYVSALRNGASTTEGHIITLR
                     DVTSIKENESALAIARDEAMQANSFKSQLLANVSHELRTPLGIILGYTDLIVRRSYGD
                     LTEKQVNILGRIKDSTQYLDGLVSELLDQAQLDSGKLQLSTRPFEPREVLGSVCNQLS
                     ILAEAKNLEFHYSISENMPISIVGDSQRLKQILVNLISNAIKFTEVGRITVNIAVLPS
                     NTEWIMQVIDTGPGIPEESLGTVFEPFKQLANAHKSLRKGYGLGLSIARQLIRLMGGN
                     ISLESALGKGTTFTITLPLVVETEDSDE"
     gene            1083..1466
                     /locus_tag="PLT08_06450"
     CDS             1083..1466
                     /locus_tag="PLT08_06450"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNE04137.1"
                     /translation="MNSFPNAIVVEDDPYLLDIYADTLRGMNIQVETYQDGMSALKGL
                     KNATPNLIILDLNLPKLSGIEIFHALRNQPQTAETWVLIITANPAQAAELSKNEIESQ
                     NLLILTKPISVDQLDQLAQRIIFHS"
     gene            1496..2503
                     /locus_tag="PLT08_06455"
     CDS             1496..2503
                     /locus_tag="PLT08_06455"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF022137.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNE04138.1"
                     /translation="MDSTFATLQPLLDQAISLRLTASSRTLNSSSHEEAFRLFNGFYE
                     GFPDLVLDVYGETLVIHDYSEAPNSVFIQELTSYIRTSLKWLRAGILKSRHGTSQKEK
                     RGVLIFGTEPNLKIKEHGIWYSIDLTMNRDASFYLDTVNLRKWLIENSRGKSVLNTFA
                     YTGSLGVAALAGEAAKVIQTDLNRQFLNLAKESCTLNGFPVEKPNFIAQDFFSVIARF
                     KAGKQAFDCVIIDPPFFSSTSRGKVDMEHESERLINKVRPLIQDGGTLIAINNAVFLS
                     GKEYMQTLEKLCKDGYLSLRELIPVPDDFMGTKRIQTPITDPAPFNHSTKIAILDVRR
                     K"
     gene            2500..2895
                     /locus_tag="PLT08_06460"
     CDS             2500..2895
                     /locus_tag="PLT08_06460"
                     /inference="COORDINATES: protein motif:HMM:NF021514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HNE04139.1"
                     /translation="MNGETELGKLLKNLKPERQPGEYVFCVVNTLKHAAELNPISTFQ
                     EAEGVTVVLPKRLADENILFYSVVCAWITLTVHSSLEAVGLTAAVSSALAGANISCNV
                     VAAFHHDHIFIPVKDAEQAMEILLSLSKG"
     gene            2897..3271
                     /locus_tag="PLT08_06465"
     CDS             2897..3271
                     /locus_tag="PLT08_06465"
                     /inference="COORDINATES: protein motif:HMM:NF024092.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HNE04140.1"
                     /translation="MNPKQVLETFWEKMESNDFYAVAELLHDEFTLEWHQSGELIRGS
                     ENFAKINTAYPANGEWRFNINSIVAEGDVVVTDVSVTDGVVKDRVITFSTVRDGKIWK
                     QIEFWPEPFAAPEWRARWVEKI"
     gene            complement(3268..>4354)
                     /locus_tag="PLT08_06470"
     CDS             complement(3268..>4354)
                     /locus_tag="PLT08_06470"
                     /inference="COORDINATES: protein motif:HMM:NF012415.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HNE04141.1"
                     /translation="IFGFYFACYDSGRMCNMMYGMKLKTTLFLALFFFALLTLSSPAK
                     AQEFGPTPTLGAPPNPADIINAVNNLRLSYGLSALYAHPVLMQIAQEQASGIAAGMPG
                     HWRPNNMTLGQWMLSLGYPLSGDLTLDGYRSENWGPASTAEDAIQMWLGDDLHTNTML
                     STERSDIGAGVAIADGQFIVVIETALQTRSGQMQSNAASILTGIPQTQIAYSNMETQA
                     AIDGVLPQYSIPVVLNTPFPNGKVYHEVLYGQTLWSIAVAYGTTIKQIQGLNNLGDST
                     TVYEGQTLLVRTDATQPAPVVTVTTENAVIPAEQTSTPAQTLLPLMETPQNSQNQRGS
                     TFGVTAIILSALFLAGVFAVMARKKEI"
BASE COUNT         1216 a         1067 c          953 g         1118 t
ORIGIN      
        1 ccaccgcgcc gtctttgaaa gtatctccga ctcggtcatt gtcctcgacc cagaactacg
       61 cattattgag cttaatcccg ccgccgtgga gatgttcaaa ttaaacattg gcgaggtgct
      121 tgggaaatct tttcaatctg tcttcagacc ttgggtgcaa ttaaatgata aagccctgca
      181 aacccatggg tatcatcgcg agatcgtatt ggaaaacgat ggcgagcctg tacgttggct
      241 acatctttat gtaagcgcct tgcgcaatgg tgcatcgacc actgaagggc atatcatcac
      301 cttacgtgat gtcactagta tcaaagaaaa tgaatctgca ttagctattg cccgtgatga
      361 agccatgcag gcaaatagct tcaaatctca attacttgcc aacgtaagcc acgaactgcg
      421 gactcctctc ggtatcatcc ttgggtacac agacctgatc gtacgtagat cctacggcga
      481 tttgaccgaa aaacaagtca acattctggg gcgcattaaa gacagcaccc aatatttaga
      541 tgggctggtt tctgaacttc tcgaccaagc ccaacttgac agcggaaaat tacagctctc
      601 cacacgtccg tttgagccgc gtgaagtgct tgggtcggtt tgcaatcaac taagcattct
      661 ggctgaggca aaaaaccttg agtttcatta ttccatttca gagaatatgc ccatttccat
      721 tgtcggagat agtcaacggc taaagcaaat ccttgtcaat ctcatcagca atgcaatcaa
      781 gttcacagaa gtcggcagga ttacggtcaa catcgcagta ttgccctcca acacagaatg
      841 gatcatgcaa gtcatagaca caggtccagg catcccagaa gagtcgcttg gcactgtttt
      901 cgagccattc aagcaattag caaatgccca taaatccctg cgcaagggtt atggcttggg
      961 gctttcaata gccaggcaac ttatccgact catgggcggg aatatctcgc ttgaaagcgc
     1021 ccttggcaaa ggcactacct tcaccatcac cctccctctt gtagttgaaa cagaagatag
     1081 cgatgaatag ttttcccaat gcaatcgtcg tagaagacga cccatacctc ttggatatct
     1141 atgccgatac tttacgcggc atgaacattc aggttgagac ctatcaagat ggcatgagcg
     1201 ccttgaaagg actcaaaaat gctacgccca acctcatcat tctcgatctc aatcttccga
     1261 aactttctgg catcgaaatc tttcatgcgc ttcgcaacca accccaaacc gctgaaacat
     1321 gggtcttgat catcaccgcc aaccccgctc aagccgccga actttctaag aacgaaattg
     1381 aaagccaaaa tctgctcatc ctcacaaagc cgatcagcgt agaccagctc gaccaacttg
     1441 ctcagcgcat tatttttcac agctaaactc accacaagcc ggatacaatc agtcaatgga
     1501 ctcaactttc gccactctgc aaccattgct agaccaagcc atttccctgc gacttaccgc
     1561 ctcttcccgg accctgaaca gctccagcca cgaagaagcg tttcgcctct tcaacgggtt
     1621 ctacgaaggc ttcccagacc ttgtgttgga tgtttatggt gagaccctag ttattcacga
     1681 ttattcagaa gctccaaatt ctgtttttat tcaagaactt acaagctata ttcgcacttc
     1741 tctaaagtgg ctacgcgctg gaattcttaa atcccgccat ggtacaagtc aaaaagaaaa
     1801 acgcggtgta ctcatctttg gtactgagcc gaacctcaag attaaagaac acggcatctg
     1861 gtactccatc gacttaacca tgaaccgcga tgcaagcttc tatcttgata ccgtcaactt
     1921 acggaaatgg ctgattgaaa actcacgcgg gaaatctgta ttaaacacct ttgcttatac
     1981 aggcagtcta ggggttgctg ctctcgcagg cgaagctgca aaagtgatcc agacggatct
     2041 taatcgtcaa ttcttgaatc ttgcaaaaga atcctgcaca ttgaacggct ttcctgtcga
     2101 aaagcctaac ttcattgcac aagacttctt ctcggttatt gcccgtttca aagctgggaa
     2161 gcaagccttc gattgcgtca tcattgaccc gcctttcttc tcttccacat cacgcggaaa
     2221 agtggacatg gagcatgaaa gcgaacggct catcaacaaa gtccgcccgt tgattcaaga
     2281 tggcggcact ctcattgcca taaacaatgc agtttttcta agcggcaaag aatacatgca
     2341 aaccctcgaa aagctttgta aagacggcta tcttagtctt cgtgaattga tccccgttcc
     2401 agatgatttt atgggaacca aacgtatcca aactcctatc actgaccctg cgccttttaa
     2461 tcattcaact aaaattgcga tcttagacgt gaggcgaaaa tgaatggtga aacagaactc
     2521 ggaaaattat tgaaaaactt gaaaccagaa cgccagcctg gcgaatatgt tttctgtgtt
     2581 gtaaacacat taaagcatgc ggctgaacta aaccccatct ctacatttca agaagctgaa
     2641 ggtgtgaccg ttgttttgcc taaacgactg gcagatgaaa atattctttt ctattcagtg
     2701 gtatgtgcat ggatcacgct caccgtccac tcttctctcg aagcggtagg gctcacagcg
     2761 gcggtcagca gtgcgttagc aggagccaat atcagttgta atgtggtggc ggcttttcat
     2821 catgaccaca tatttatccc ggtcaaagat gcagaacagg cgatggaaat tctgttatcc
     2881 ctatcaaaag gctaagatga atcctaaaca ggtcttagaa acattttggg aaaagatgga
     2941 aagcaacgat ttttatgcag ttgctgaact cttgcacgac gaattcacgc tcgaatggca
     3001 tcaatcaggt gaattgatcc gcggcagtga aaattttgca aagatcaata cggcataccc
     3061 agccaatgga gagtggcgct tcaatatcaa ttccatcgtt gctgaagggg atgttgttgt
     3121 aacagatgta tcagtcacgg atggggtggt aaaagataga gtcatcactt tttcaacagt
     3181 tcgcgatgga aagatctgga aacagatcga attctggccc gaaccattcg cagcaccaga
     3241 atggcgagcc cgatgggtgg aaaaaatcta gatctctttt ttacgcgcca ttacagcaaa
     3301 caccccagca agaaataaag cagaaagaat aatcgcagtc acaccaaacg tggacccacg
     3361 ctgattctga gagttttgtg gtgtctccat taaaggcagg agcgtttgtg caggtgttga
     3421 tgtctgctcg gcgggaatca ctgcgttctc agttgttacg gttacaacag gtgcaggttg
     3481 ggtggcatct gttcggacaa gcagtgtttg cccttcataa acagttgttg agtctcctag
     3541 attatttagt ccctgaattt gcttaatcgt cgttccgtaa gctacagcaa tgctccataa
     3601 ggtttgccca tacaatactt cgtgatacac cttgccattc gggaagggag tattcaatac
     3661 cacgggaatg gaatattgcg gcaatacgcc atcgatcgca gcttgtgtct ccatgttgga
     3721 atatgctatt tgcgtttgag gtattcctgt aaggatcgaa gccgcgttcg attgcatttg
     3781 tccactacgg gtttggagtg cggtttcgat caccacgata aattgtccat cggcaatggc
     3841 gacacccgct ccaatatcac ttcgctctgt tgagagcatg gtatttgtgt gcaggtcatc
     3901 tcccaaccac atttgaattg catcctctgc ggttgatgcg ggtccccagt tttcggagcg
     3961 atagccgtcc aaggtcaggt caccagacag tggatagccc aatgagagca tccactgtcc
     4021 caacgtcatg ttattaggtc gccagtgacc gggcatacca gcagcaattc cgctggcttg
     4081 ttcttgggca atctgcatta atacggggtg ggcatacaat gctgacaacc catatgaaag
     4141 ccgcaaatta ttaaccgcat tgataatatc cgccggattg ggtggagcac ctaatgttgg
     4201 agtaggtcca aactcctgcg ctttggctgg agaagataag gttaataacg caaaaaagaa
     4261 taaagcaaga aatagtgtgg tttttagttt catcccatac atcatgttgc acatcctacc
     4321 tgaatcataa caggcaaaat aaaagccgaa gatt
//
LOCUS       DAOQJE010000190         4311 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_12314_length_4311_cov_4.697368, whole genome shotgun sequence.
ACCESSION   DAOQJE010000190 DAOQJE010000000
VERSION     DAOQJE010000190.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 4311)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4311)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4311
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12314_length_4311_cov_4.697368"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..861
                     /locus_tag="PLT08_06475"
     CDS             <1..861
                     /locus_tag="PLT08_06475"
                     /inference="COORDINATES: protein motif:HMM:NF027964.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="saccharopine dehydrogenase C-terminal
                     domain-containing protein"
                     /protein_id="HNE04142.1"
                     /translation="PFNKPGVKLGDYQFEKAKEWEKKGLLALVGFGVEPGMADVFARY
                     AADHLFDEIEEVGIRDGANITIEGYDFAPNFSIWTTIEECLNPPVIWQNGDWHTTAPF
                     SEPEVFDFPEIGPVEVVNVEHEEVLLVPRWVKAKRVTFKYGLGNEFIEVLKTLHKLGL
                     DNKNKINVKGVQVAPRDVVAACLPDPAHLGDKMKGKTCAGTWVKGTKDGKKKEVYLYQ
                     VADNEECMKTWGCQAVVAQTAFTTVIAMDLLEHGAWKGTGVLGPEAFEPTVFMEKMAD
                     YGFPYGIKNM"
     gene            1080..2987
                     /locus_tag="PLT08_06480"
     CDS             1080..2987
                     /locus_tag="PLT08_06480"
                     /inference="COORDINATES: protein motif:HMM:NF013481.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde ferredoxin oxidoreductase family
                     protein"
                     /protein_id="HNE04143.1"
                     /translation="MPNGYNGKILHVDLTKGSLTVEEPNEAFYRKYLGGSAMGMHYIL
                     RDMPKGADPLGPENVLTLFTGVTTGAAISGQSRLNANAKSPISGGIGDSQSGGFFPAE
                     LKFAGFDGIVVKGKSPKPVYLCIIDGKFELRDASHLLGKLSGEVDDIIHKEVDPKAEI
                     LQHGIGAENGVLYSSLVSMSNRHNGRTGMGLVMASKNLKAVVVRGTGKIKLANQKALT
                     ELNRIGPKAIPENGDMDGLAKFGTAVVVLFNNTIGTLPTRNYNEGQFEGCEPISGEKM
                     AETVLKERDTCYACVVKCKRVVEIKDGPYKVDPRYGGPEYETLGTFGSYCGVDDLAAV
                     SLANQICNEYAVDTIAAGATIAFAMECYEKGIITKEQTAGLDLKFGNAEAMIAALNML
                     VKGEGEFGKTLGLGSERAAAKWGNGADECLITVKGAEAPAHMPQAKRSLALIYAVNPF
                     GADHQSSEHDPYYEEGVGDFNLDRLKQIGLGSPQPAYSLTEEKVRFAYETEVFYSMLD
                     SAELCQFVYGPTWTVYDGEQTAQMISSVTGWDITLNELMEVGRRRLDLFRVFNAREGL
                     ARNADKLPKKFFKELKGTGPTAGFALTHEEVESALDTYYKMAGWTNNGIPTRETLKKH
                     DVEWAAEYLPA"
     gene            3075..>4311
                     /locus_tag="PLT08_06485"
     CDS             3075..>4311
                     /locus_tag="PLT08_06485"
                     /inference="COORDINATES: protein motif:HMM:NF012428.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HNE04144.1"
                     /translation="MTNQLYQDNFSHVTPAWSRIFNFVADRAEGSYIYTDDGKKLLDF
                     TSGIGVTNTGHCHPKVVEAIREQAGLFLHAQANIVIHKPMLQLIEELRKVVPPSIDSF
                     YFANSGAEALENAVKIAKAVTGRQNIIVFNGSFHGRTHATMALTTSKTIYRTGFAPLP
                     AGIYVAPFPYAFNLKMTEEQASQYALEQLEYLLASQTAPKETAAILIESVLGEGGYVV
                     PPASFMKGLREICDKHGIMLIFDEVQSGFGRTGKWFALEHFGVVPDIITAAKGIASGM
                     PLSGVFSRTEIMKKVDTGSIGGTYGGNAVACAAGVATIRAMREEKMLENATERGIQLM
                     TGLHKLQEEYPQIGDVRGKGLMVGTEFIVDGSQAKAKPLVKEIIHKAEEKGLLLLSCG
                     TYDNILRWIPPLNVSSEQIN"
BASE COUNT         1103 a          912 c         1198 g         1098 t
ORIGIN      
        1 ccgttcaata aaccgggggt taagttgggc gattatcaat ttgagaaagc caaagagtgg
       61 gagaagaaag gtttgttggc gcttgttggg tttggcgtgg aacctggcat ggcagatgtc
      121 tttgctcgct atgcagctga ccatctcttt gacgagatcg aagaagtggg cattcgcgat
      181 ggcgctaata tcaccattga aggctatgat tttgcgccga atttttcaat ttggacaacc
      241 attgaagaat gtttgaatcc acccgtgatt tggcaaaacg gagattggca taccacggct
      301 ccgttctctg agcctgaagt gttcgacttc cctgaaattg gacccgttga agtcgtcaac
      361 gtggagcatg aggaagtatt gctcgtgccg cgctgggtga aggcaaagcg tgtgaccttc
      421 aagtatgggt tggggaatga atttattgaa gtgttgaaaa cccttcataa acttggcttg
      481 gataataaga acaagatcaa tgtgaaaggc gtgcaagttg cgccgcgaga tgtggtggcg
      541 gcttgtttac cagacccagc tcacttgggt gacaagatga aaggcaagac atgtgccgga
      601 acctgggtaa aaggcacaaa agatgggaag aagaaagaag tatatctata tcaggttgcg
      661 gataatgaag aatgcatgaa gacttgggga tgtcaggcag tggtggctca aacggcattt
      721 accactgtga ttgctatgga tttgctggaa catggcgctt ggaagggcac aggtgtgctc
      781 ggacctgagg cgtttgagcc gactgtcttc atggaaaaaa tggcagatta cggcttccca
      841 tacggcatta agaatatgta gtttataaaa gggcgactct ttacgggacg cccttttgct
      901 tcgggtgtga aaggttcgag aaagcggtat ccgctgtcag ttaggaagga agcgcggcgg
      961 ggagtgtagg aaaagactta atcgaagcgg ttaagctgtc gctttcggaa actgcctgtt
     1021 gtgagaagtc agacccgctt gttataatta ccatcatcaa ttcttacgga ggagtaacca
     1081 tgccaaacgg ttataacgga aagattttgc acgttgatct gacgaagggc tctctgacgg
     1141 tggaggagcc gaatgaggct ttttaccgca agtatttagg cgggagcgca atgggaatgc
     1201 actacatctt gcgcgatatg ccgaagggcg ctgacccgct tggacctgaa aatgttttga
     1261 ccttgtttac cggtgttaca actggggcgg caatctcagg tcagagccgt ttgaatgcaa
     1321 atgccaagtc gccgatcagt ggcggtattg gtgattcgca gagcggtggg ttcttcccgg
     1381 ctgaattgaa gtttgccggt tttgatggca ttgttgtgaa ggggaagtca cccaagcctg
     1441 tatatctctg cattattgat ggaaaatttg aattacgcga cgcctcgcat ttgctgggca
     1501 agctttcggg cgaagtggac gatatcatcc acaaagaagt ggacccgaag gctgagatcc
     1561 ttcagcatgg gattggcgct gaaaatgggg tactgtattc atccctcgtt tctatgtcta
     1621 atcgtcataa tgggcgtacg ggcatgggtt tggtgatggc atctaagaat ctcaaggctg
     1681 tggtggtgcg aggtaccggc aagattaaac ttgccaatca aaaggctctt actgagttaa
     1741 atcgtatcgg tccgaaagcc attcctgaaa atggtgatat ggacgggctt gcgaaattcg
     1801 gtacggcagt ggtggtattg ttcaataaca ccattggtac attgccaacc cgtaactata
     1861 acgaaggtca gttcgaaggt tgtgaaccga tcagcggcga aaaaatggct gagacggtct
     1921 tgaaggaacg tgatacttgc tatgcatgcg tggtcaaatg caaacgcgtg gtagagatca
     1981 aagacggtcc ttataaggtt gatccgcgct atggtggtcc tgagtacgaa actcttggca
     2041 cgtttggttc gtactgcggt gtggatgatc ttgcggcggt atctcttgcc aaccagattt
     2101 gtaatgaata tgccgtagat acaattgcgg ctggtgcaac catcgctttt gcgatggagt
     2161 gctatgaaaa aggcattatc accaaagaac agaccgctgg tcttgacttg aaatttggta
     2221 atgccgaagc aatgatcgct gctttgaata tgctggtgaa gggtgagggt gaattcggca
     2281 aaacccttgg tttgggctca gaacgtgctg ccgccaagtg gggaaatggt gcagatgaat
     2341 gcctcatcac ggtcaaaggc gctgaagctc ctgcacacat gccgcaagcc aaacgctcgt
     2401 tggcgctcat ttatgcggtc aatcccttcg gcgcagatca ccaatcttct gaacacgacc
     2461 cgtactatga agaaggtgta ggtgatttca acctcgaccg cctcaaacag attggtttgg
     2521 gttcgcccca acctgcatac tcacttacag aggaaaaagt ccgcttcgca tacgagaccg
     2581 aagtctttta ctcgatgctc gactcggcag aactctgcca gtttgtgtac ggtccgacat
     2641 ggacggtcta tgatggcgaa caaactgcac agatgatcag ctcggtaacg ggttgggata
     2701 ttaccctaaa cgaattgatg gaagtcgggc gccgacgttt ggatctcttc cgtgtcttca
     2761 atgctcgtga aggtttggct cgcaatgctg ataaattgcc taagaaattc ttcaaggaat
     2821 tgaaggggac aggtcccaca gcgggcttcg ctctgactca tgaagaagtt gaatctgctc
     2881 tcgataccta ttacaagatg gcaggttgga cgaataacgg cattcctacc cgcgaaactt
     2941 tgaagaagca tgatgtagag tgggcagcgg aataccttcc ggcataatgt aaatacaatc
     3001 tgatcactga gagcacagag tccaagaaga aattcaggga aaactctgtg ctctttctgg
     3061 tcaataagga aatcatgacc aatcaactct atcaagataa tttttctcat gtaacccctg
     3121 cctggagccg cattttcaat ttcgtagcgg accgtgctga gggttcatat atttataccg
     3181 atgacggcaa gaaacttctg gatttcacca gtggaattgg cgtcacaaac acggggcatt
     3241 gtcaccccaa agtggtggag gcgattcgtg agcaggctgg cttgttcttg catgctcagg
     3301 cgaacatcgt catccacaaa ccgatgttgc aactgattga agaactgcgt aaagtagttc
     3361 cgccttcgat tgatagtttc tactttgcca actctggcgc agaggcgttg gaaaatgccg
     3421 tgaagattgc caaggctgtc acgggtaggc aaaacattat tgtcttcaac ggctcctttc
     3481 atgggcgcac gcatgccact atggcattga ccacctctaa gacaatctat cgcaccggct
     3541 ttgcgccttt gcctgctggc atttacgtag cgccgttccc atatgctttc aacctgaaga
     3601 tgaccgaaga acaagccagc caatatgcgt tagaacaatt ggaatatttg ctagcttctc
     3661 aaacggctcc taaagagacc gccgctatct tgatcgagtc agtattgggt gagggcgggt
     3721 atgtggtgcc gcctgcctcc ttcatgaagg gattgcgtga gatctgcgac aagcacggca
     3781 ttatgctgat tttcgatgaa gtgcaatctg gttttggtcg cacgggcaag tggtttgcgt
     3841 tggaacactt tggtgttgtt ccagatatta tcactgctgc aaaagggatt gcctctggaa
     3901 tgccgctttc gggcgtcttt agtcgcaccg agatcatgaa gaaagtggat actggctcca
     3961 ttggtgggac ctatggtggg aacgcagttg cctgcgcggc tggcgttgct accatccgcg
     4021 cgatgcgtga agagaagatg ttggagaacg ccacggaacg cggaatccaa ttgatgaccg
     4081 ggcttcataa attgcaggaa gaatatccgc aaattggtga tgtgcgcggc aaaggcttaa
     4141 tggttggtac cgaatttatt gtggatggca gtcaggcgaa agccaagccg ttggtcaaag
     4201 agatcatcca taaagcagaa gagaagggtt tgctccttct ctcctgtggt acctacgata
     4261 atattttgcg ttggattccg ccattgaatg tctcttctga acagatcaac g
//
LOCUS       DAOQJE010000191         4298 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_12370_length_4298_cov_5.744520, whole genome shotgun sequence.
ACCESSION   DAOQJE010000191 DAOQJE010000000
VERSION     DAOQJE010000191.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 4298)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4298)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4298
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12370_length_4298_cov_5.744520"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..311
                     /locus_tag="PLT08_06490"
     CDS             <1..311
                     /locus_tag="PLT08_06490"
                     /inference="COORDINATES: protein motif:HMM:NF012797.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HNE04145.1"
                     /translation="RSGMGAHFRLPIHAMTWAEIEKTTNGLQVYVADMDGQSCWDVDL
                     SKPIALIVGNEAEGASESARKLANGKISILMSGETESLNAGVAGSVLMFEILRQRSNV
                     "
     gene            351..1568
                     /locus_tag="PLT08_06495"
     CDS             351..1568
                     /locus_tag="PLT08_06495"
                     /inference="COORDINATES: protein motif:HMM:NF017019.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF711 family protein"
                     /protein_id="HNE04146.1"
                     /translation="MKIRSITYFCNPGYPLKENILREAGDFLSKAKSAYETAGYEVQT
                     VRVATVSFAKLLGEKHIDGLPKYAEQFTQVMKQNGVVYAALGPALPELPRSYQVIPDA
                     IKASEGVFFSGVMADAQTIHMAQVKACAEIIVKSAPLDPNGFANLHFTALANVGANAP
                     FFPGAYHTSDQPAFGIAVECADMAVDAFANAQSIEEGRQWLVSEMTKHGQAITNIANG
                     LQIEFKGIDFSLAPFPDDAHSLGKAVENMGVPKIGLHGSLAAAAILTDAIERADFPHI
                     GFNGFMQPILEDSILAKRAAEGALTVKDALLYSAVCGTGLDTVPLAGDVTADELTPLL
                     LDLCALALRLNKPLTARLMPVPGKKAGDATTFDFAFFANGRVMALESQPLSLPFNSNE
                     SFSLKPRAERNRP"
     gene            1658..3334
                     /locus_tag="PLT08_06500"
     CDS             1658..3334
                     /locus_tag="PLT08_06500"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional metallophosphatase/5'-nucleotidase"
                     /protein_id="HNE04147.1"
                     /translation="MKRNILIIGGLVFVAIVAFFVYKAVAPSKPVNVQLLALNDFHGN
                     LLPPSGSSGQVDGVDAGGVEYLATHIDTLRGENPNTVVVSAGDLVGASPLLSALFHDE
                     PTIEAFNLIRLDFNAVGNHEFDEGWAELKRLQEGGCHPVDGCLDGDDFSGAEFKFLAA
                     NVINKETGEPIFPAYEVREFEGVKIAFIGMTLKGTPSIVTPSGVADLEFLDEAETVNA
                     IIPELQAQGIETIVVLVHEGARQNGDAPTANTCQDIDGPIVDIVSRFDDAVDVVISGH
                     THQAYNCLLDGKLLTSAMSFGRLVTQIHLTIDPKTGDVTTMEANNLVVTRDVEKASLL
                     TALIERYNKVAEPLANHEVGTITADILREPNDAGEMALGDVIADSQLFATSASDMGNA
                     VIAFMNPGGIRTDLVYADSVGEGDGTVTFGEIFTVQPFNNNLVTLTLTGEQIDALLEQ
                     QFVDCGQTENPRILQVSKGFSYTWKASGAPCEKVDPATIFLNDEPIDPAKEYRVTVNS
                     YLADGGDRFKVLTEGTDRLVGVMDLDALEAYMLQASPVEPGAQDRIKVIP"
     gene            complement(3375..3932)
                     /locus_tag="PLT08_06505"
     CDS             complement(3375..3932)
                     /locus_tag="PLT08_06505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HNE04148.1"
                     /translation="MSEELVAFFKVLSDANRLKIIGLLAHQPYSVEELAALLGLKPST
                     VSHHLSKLSKVGLVSAKTESYYNVYQLDQKALEEKSRSLFSQDKMTASVADVDVDAYD
                     RKVIGDYTRKDGSLKTIPAQRKKLDAVLRHIVKAFKVGKRYSEKQVNEILSTYHDDTA
                     SLRRELVGAKLMQREGGGGEYWRDE"
     gene            complement(3934..>4298)
                     /locus_tag="PLT08_06510"
     CDS             complement(3934..>4298)
                     /locus_tag="PLT08_06510"
                     /inference="COORDINATES: protein motif:HMM:NF013691.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HNE04149.1"
                     /translation="KTRKELNREYAERVKPAGIYQVKNTVSGKMFLGSSLNLEGPLNR
                     HRFMLKIGSHTNKALQNDWNQLGADAFAFEILEEVKMKDDPNFNLHDELTLLEMIWLE
                     KLQPVENGYNLNARIREA"
BASE COUNT         1087 a          980 c         1090 g         1141 t
ORIGIN      
        1 tgcgatctgg tatgggagcg catttcaggc tgcccattca tgcaatgact tgggcagaga
       61 ttgagaaaac tacaaatggc ttgcaagttt acgtcgccga tatggatgga caatcttgct
      121 gggatgtgga cttatccaag ccgattgctt tgatcgttgg caacgaagca gaaggggcaa
      181 gcgagtccgc gcggaagttg gcgaatggga aaattagcat tctaatgtcc ggcgagactg
      241 agtctctgaa tgcgggagta gcggggtcgg tgttgatgtt tgagatatta aggcaacgtt
      301 ccaacgtttg aacgtttata ggttttcaat cttctactgt atgaggaaaa atgaaaatac
      361 gatccattac ctatttctgt aatcctggtt atcccttgaa agaaaatatc ctgcgtgaag
      421 cgggagattt tttatctaaa gccaagtctg cgtatgagac ggctgggtat gaagtacaaa
      481 ctgtgcgtgt ggcgacggtt tcgtttgcga aactgctggg tgaaaaacat atcgatggtc
      541 tgccgaaata tgccgagcag tttacacagg tgatgaaaca aaacggcgtc gtgtatgcag
      601 cgctgggacc tgctctgcca gaattgccac gcagctatca agtcattcca gatgcgataa
      661 aagcaagtga aggcgttttc ttcagcggcg taatggcaga tgcgcagacg atccacatgg
      721 cacaagtcaa ggcatgtgca gagatcatcg tcaagtctgc accgttagac ccgaatgggt
      781 ttgccaatct gcatttcact gcgttggcaa atgtcggtgc gaatgcgcca ttcttcccgg
      841 gcgcgtatca tacgtctgac cagcctgcgt ttggaattgc cgtcgaatgt gctgatatgg
      901 cagtggatgc ctttgcaaac gcccaatcca ttgaagaagg gcgtcaatgg ctcgtttcag
      961 aaatgaccaa acatggtcaa gccattacga acatcgcaaa cggtttacaa atcgaattca
     1021 aaggcatcga cttctcgctt gcacccttcc ctgatgatgc acattcgctg ggcaaagccg
     1081 tagagaatat gggcgtgccg aagatcggtt tacatggttc gcttgcagca gcagcaattc
     1141 tcaccgatgc catcgagcga gcagactttc cgcatattgg gttcaatggt ttcatgcaac
     1201 ccattctcga agattctatt ttggcaaagc gtgccgccga aggtgcgctg accgtgaagg
     1261 atgctttgct ttattccgcc gtgtgcggca caggtttgga tactgttccg ttggctgggg
     1321 atgtaaccgc agacgaactc acgcctcttc tcttggacct gtgtgctttg gcgcttcgat
     1381 tgaacaaacc cctcaccgcc aggctgatgc ccgtcccagg taaaaaagcg ggagatgcaa
     1441 ccacctttga cttcgctttc ttcgccaacg gacgtgtgat ggcattagag tcacagccgc
     1501 tgagcttgcc tttcaatagc aatgagtcat tttcgctgaa gccaagggca gagagaaacc
     1561 gtccctgaag aaaaggttga agaggggctg ccagttttcg gttaagaatg aagcgggttt
     1621 tcatcctgtt tcaaatagtt caagaggaaa ataatcaatg aaaagaaata tcctaattat
     1681 cggtggattg gtttttgtcg caatcgttgc gttttttgta tataaagccg tggcaccatc
     1741 taagcctgta aatgtgcaat tgctggcatt gaatgacttt catggaaatt tgttgccgcc
     1801 ttctggctct tcaggtcagg tggacggtgt ggatgcaggc ggcgtagagt atctggcaac
     1861 gcatatcgat acgttgcgcg gtgaaaaccc aaatacggtt gtggtttctg ctggtgatct
     1921 ggtgggagcc agcccgctgc tttctgcgct ctttcacgat gaaccgacca ttgaagcctt
     1981 taatttgatc aggttggatt tcaatgccgt tggcaaccat gagtttgacg aaggctgggc
     2041 ggagttaaaa cgtttgcaag agggtggttg tcaccctgtg gatggatgtt tggatggcga
     2101 tgatttttca ggagccgagt ttaaattcct tgcggcgaat gttatcaaca aagagactgg
     2161 cgagccgatc tttccagcct atgaggtgcg cgagttcgaa ggcgtaaaaa tcgctttcat
     2221 tggcatgacg ctcaaaggaa cgccaagcat cgtcacgcct tctggcgttg cagatttgga
     2281 atttctggat gaggctgaaa cggtgaatgc gattatccct gaattgcaag cgcaagggat
     2341 tgagaccatt gtagttttgg ttcatgaggg cgcacgccaa aatggagatg ctcccactgc
     2401 aaatacctgt caggatattg acggaccgat cgtggatatt gtcagccgat ttgatgatgc
     2461 cgtggatgta gtcatcagtg gacatacgca tcaggcatat aactgtttac tggatggaaa
     2521 actgcttacc agcgccatgt cgtttgggcg gctcgtgacg caaattcatt tgaccattga
     2581 ccccaagacc ggtgatgtaa ccaccatgga agccaataat ttggtcgtga cccgcgatgt
     2641 ggaaaaggcg tcattactaa ctgcattgat cgaacgctat aacaaagttg cagaaccgct
     2701 tgccaatcat gaggtgggca ctatcaccgc agatattctg cgcgaaccca atgatgcagg
     2761 tgaaatggca ttaggcgatg tgattgcaga ttcacagctc tttgccacaa gtgcctctga
     2821 tatgggcaat gccgtgattg cctttatgaa cccgggcggc atccgcacgg atttggtata
     2881 tgccgattct gtcggtgaag gggatggcac ggttactttt ggtgagattt tcaccgttca
     2941 gccgttcaac aacaatctgg ttacgctcac tctaaccggt gaacagatcg atgctttact
     3001 tgaacagcaa tttgtagatt gcgggcagac tgagaatccg cgcattttgc aggtctctaa
     3061 aggcttctcg tatacatgga aagcgtctgg cgctccatgc gaaaaagtgg accctgctac
     3121 aatcttttta aatgatgaac caattgaccc cgcaaaagaa tatcgtgtca cggtcaactc
     3181 gtatttagca gatggcggtg accgcttcaa agtgttgaca gaaggaacag atcgcctcgt
     3241 aggtgtaatg gaccttgatg ctttggaagc ctacatgctc caagcttcac cagtagaacc
     3301 cggggcacaa gatcgaatta aggttattcc ataaacacga ataaaaagag actctggcaa
     3361 gccagagtct ctttttattc gtctcgccaa tactctccgc caccgccctc acgctgcatg
     3421 agctttgctc ccaccagttc acggcgtaaa gaagctgtgt catcatgata ggtgcttaga
     3481 atctcattga cctgcttttc gctataccgt ttgccaactt tgaaagcttt tacgatgtga
     3541 cgcagaaccg catccaactt cttacgctga gcagggatgg ttttcaagct accatctttc
     3601 cgagtgtaat caccgattac tttacgatca taagcatcca cgtccacatc ggcaacagag
     3661 gcggtcattt tgtcttgaga aaacaggctg cgagattttt cttccagcgc cttctggtca
     3721 agttgataaa cgttgtaata actttccgtc tttgcgctca ccaagccaac ctttgaaagc
     3781 ttggacaagt gatgcgagac agtagaaggt ttcagcccca ataaagcagc gagttcttcc
     3841 acactatacg gctgatgcgc aagcagtcca atgatcttga ggcgattcgc atccgaaagg
     3901 actttgaaaa aggcgaccaa ttcttcgctc atatcacgcc tcccggattc tggcattcag
     3961 gttgtagcca ttttcaacag gctgaagctt ctcaagccag atcatttcca gcagagttaa
     4021 ctcatcgtga aggttaaaat tcgggtcgtc tttcatcttt acttcttcaa ggatttcaaa
     4081 ggcaaaggca tctgcaccca attgattcca atcattttga agagccttat tggtatggct
     4141 tccaatcttt agcatgaagc gatggcggtt caacggacct tcaaggttta ggctactgcc
     4201 aagaaacatc ttgccgctta ctgtattttt cacctgataa atcccagccg gcttaacacg
     4261 ttctgcatat tcgcgattga gttctttacg ggttttca
//
LOCUS       DAOQJE010000192         4280 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_12460_length_4280_cov_6.240237, whole genome shotgun sequence.
ACCESSION   DAOQJE010000192 DAOQJE010000000
VERSION     DAOQJE010000192.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 4280)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4280)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4280
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12460_length_4280_cov_6.240237"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..404
                     /locus_tag="PLT08_06515"
     CDS             <1..404
                     /locus_tag="PLT08_06515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE04150.1"
                     /translation="EERFPERAPILYTEAGRAAVLNGDAKKGVTHFRSALTLLSTQQR
                     YRRLQNLGERIVAELRERGLNAEADEVASVLRNNAQVSAGKEAPAPQKRAVLPTHCPS
                     CGAAVRPDEVEWLDDVTAECDYCGSPVRAES"
     gene            406..1005
                     /locus_tag="PLT08_06520"
     CDS             406..1005
                     /locus_tag="PLT08_06520"
                     /EC_number="3.6.1.55"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005777 - peroxisome [Evidence IEA];
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003986 - acetyl-CoA hydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0008413 - 8-oxo-7,8-dihydroguanosine
                     triphosphate pyrophosphatase activity [Evidence IEA];
                     GO_function: GO:0010945 - CoA pyrophosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0035539 - 8-oxo-7,8-dihydrodeoxyguanosine
                     triphosphate pyrophosphatase activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0009132 - nucleoside diphosphate metabolic
                     process [Evidence IEA];
                     GO_process: GO:0015938 - coenzyme A catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA pyrophosphatase"
                     /protein_id="HNE04151.1"
                     /translation="MTEITEEYIWNKLNTTDRSRVETDGYAEIPIHPDVQLKCAAVLV
                     PLVFFQNEWHVLYTRRTDRVESHKGQVSFPGGACDEGETTPEETALREADEEIGLNPK
                     DVKVIGKLSRMVTISKFKVTPVVGIIPFPYAFKTSGAEVARVFTMPLLWLANRNNYWE
                     FSMPGSGRTLLAYHPYDGELLWGATARMTVNFLSLLDLL"
     gene            1227..2042
                     /locus_tag="PLT08_06525"
     CDS             1227..2042
                     /locus_tag="PLT08_06525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HNE04152.1"
                     /translation="MKVQNKVMVVTGGGNGMGRELVLNLLAKGARVAAIDINETALKE
                     TAQLAKEYKENIATFVLNITDKDAVDALPAKVIAKFGKVDGIINNAGIIQPFVRLVDL
                     DYSAIQRVLDVNLYGTIYMVKAFLPHLKERPEAHITNVSSMGGFLPVPGQTVYGASKA
                     AVKLLTEGLYAELLNTNVKVTLVYPGAIGTNIAANSGITVPNMPEADSKEASNFKQLA
                     PDKAAEIIVNAIENNRYSVLVGSDAKFMDFIYRLNPQNAAKFIYNQMKALLPS"
     gene            complement(2108..2752)
                     /locus_tag="PLT08_06530"
     CDS             complement(2108..2752)
                     /locus_tag="PLT08_06530"
                     /inference="COORDINATES: protein motif:HMM:TIGR00731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0008097 - 5S rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25"
                     /protein_id="HNE04153.1"
                     /translation="MEKVVLKATKRDVIGKQVKAMRREGKLPAVIYGRHLEPISIVLD
                     AHTAGLALSKVSASSLVTIDVEGKEYPALVRERQRDFIKGVLTHVDFLAVDMNEKIRT
                     SVGLAFVGVSGGVKDYNGILVHNLERLEVECLPGDLPERINVDISLLKQIGDIFRVKD
                     LAISEKVRILADEEEPIAVVTITKDEASAQSAAAGTGEATSPELSVERGKKDEE"
     gene            complement(2856..3374)
                     /locus_tag="PLT08_06535"
     CDS             complement(2856..3374)
                     /locus_tag="PLT08_06535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE04154.1"
                     /translation="MTETNPSSEQNPQVTNLSQVDVETVNAQLVRMHQSSAGLVTSED
                     VDMTMSAVAQAKVTNLKLTDSGIAALHADTANVQNSVVGVAQTTELTLNGVSGAVVAG
                     SVEFGNAYAGIVAGREVRAERIESLILLSPNVQGNVTTVMDTRGAIIAGLVSGLFAGI
                     MLLLGRMLFGKK"
     gene            complement(3426..4067)
                     /locus_tag="PLT08_06540"
     CDS             complement(3426..4067)
                     /locus_tag="PLT08_06540"
                     /inference="COORDINATES: protein motif:HMM:NF016207.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding negative transcriptional regulator"
                     /protein_id="HNE04155.1"
                     /translation="MHIPKYYREEDQDKIVEFLKANSFPAIVSSDGEKLLATHLPVEV
                     IENADGSLTVLGHMSRANPQWKSFGEQEVLLIFQGAHTYISPRWYNHVNVPTWNYINV
                     HVYGKVRMLEGEGLHSLLSKLVSKHETGTGYSMEGLPADFVEKEMRGVVGFALDVTRL
                     DAASKLSQNRDDESYANIISHLEARGDDISAEIAHEMRDRRPSLTNPPQTDKI"
     gene            4129..4263
                     /locus_tag="PLT08_06545"
     CDS             4129..4263
                     /locus_tag="PLT08_06545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE04156.1"
                     /translation="MSKRYKKKRSTTPAWVDSLIVILEILGAIGTVIAGIYSLLTWVF
                     "
BASE COUNT         1119 a         1073 c         1031 g         1057 t
ORIGIN      
        1 ctgaagaacg cttccctgaa cgagcaccga tcctttatac agaagcgggt cgtgctgcgg
       61 ttttgaatgg cgacgcaaaa aaaggcgtta cccattttcg tagtgcgctg accttactaa
      121 gcacacaaca acgttatcgc cgtttgcaga accttggcga gcgcattgta gcggaattac
      181 gtgaacgcgg cttgaatgcc gaagctgacg aagtagcatc cgttctcagg aacaatgccc
      241 aagtttcggc ggggaaagaa gcgccagcac cgcaaaagcg agcggtcttg ccaacccatt
      301 gcccatcgtg tggtgcggca gtccgcccgg atgaagtcga atggctggat gatgtgaccg
      361 cagaatgtga ttactgcggc agcccggtgc gcgctgaatc ttagtatgac cgagattaca
      421 gaagaataca tttggaataa acttaatacg accgaccgtt cgcgcgtgga aacggacggg
      481 tatgccgaga ttccgattca tccagatgtg cagttgaaat gcgcggcggt tctggttccg
      541 ttggtattct tccaaaatga atggcatgtg ctatacaccc gccgcacaga ccgggttgaa
      601 tcgcacaaag ggcaagtctc ttttcccggc ggtgcttgcg atgaaggcga aaccacgccc
      661 gaagagaccg ctctacgcga agccgatgaa gagatcgggt tgaatcctaa agatgtaaaa
      721 gtgattggca aactcagccg catggtgaca atcagtaaat tcaaagtgac ccctgtggtt
      781 gggatcattc cttttcctta tgcgtttaag acctctggtg cagaagtggc gcgggtcttt
      841 accatgcctt tgctttggct cgccaaccgc aataactatt gggaattttc catgcccggc
      901 tctgggcgga ctttgctcgc ctatcaccca tatgacggtg aacttctctg gggcgccaca
      961 gcacgcatga cggtcaactt cttgtctctc ttagatttgc tgtagtttct ccagatatat
     1021 aacttgaagt tagatttagg acaatttcca ccctctatat tgtgaaaaat atcacaataa
     1081 tcctctttat ttgtgattaa tttcactaat aaaatctctc cctcagacct acaatacaga
     1141 cgatcagaac gatcaaacaa caatctcggt gcccctcccc ttatggaggt gaaagcaacc
     1201 caaggattga taaaggagag atcaacatga aagtacaaaa taaagttatg gtagtaacgg
     1261 gcggcggaaa cggtatggga cgtgagttgg tgttgaacct tttagcaaaa ggcgcacgtg
     1321 tggctgccat cgatatcaac gaaactgctt tgaaagaaac cgctcaattg gcgaaagagt
     1381 acaaagaaaa tatagcgaca ttcgtcttga acattacaga taaagacgct gtcgatgcgt
     1441 tgcctgccaa agtgattgcc aaattcggaa aagtagatgg catcatcaac aacgcgggca
     1501 ttattcagcc tttcgtgcga ctcgttgact tagactattc cgccattcaa cgtgtattgg
     1561 atgtaaacct gtacggcacc atctacatgg tcaaagcttt tctgccgcat ttgaaggaac
     1621 gccctgaagc tcacatcacc aacgtttcca gcatgggcgg gttcttgccg gtccctgggc
     1681 aaacggttta tggcgcgagc aaggctgcag ttaagttgct aaccgaaggt ttatatgcag
     1741 aattgctgaa caccaacgta aaagtgacct tggtgtaccc gggcgccatt ggtacaaaca
     1801 tcgctgccaa ttcgggcatc acagtaccga acatgccaga agcggacagt aaagaagcca
     1861 gtaactttaa gcagttagcg ccagacaaag cggcagaaat catcgtgaat gccatcgaga
     1921 ataatcgcta cagcgttctc gtaggctcag atgcaaagtt catggatttc atctacagac
     1981 tgaatccgca aaatgcagcc aagtttatct ataaccaaat gaaagccttg ctgccttcgt
     2041 aatacattgg taaaaaaagt ccgccatctt gcgatggcgg acttttttat tgctagtaca
     2101 gaagaaatta ttcttcgtct ttcttgccac gttccacgct gagctcggga gacgtcgctt
     2161 cgccggtgcc ggcagcagca gactgggcag aagcctcatc cttggtgatg gtcaccacgg
     2221 caatgggttc ttcttcatcc gccaagatgc gaactttttc ggagatcgcg aggtctttca
     2281 cgcggaagat atcgccgatc tgcttgagca gagagatatc tacattgatg cgttcaggaa
     2341 gatcacctgg taaacattcc acttcgaggc gttccaagtt gtggacgagg ataccgttgt
     2401 aatccttcac gccgccagaa acacccacga aagcgagtcc cacgctggtg cggatctttt
     2461 cgttcatgtc cacggcgagg aagtcaacat gggtaagaac acctttgata aagtcacgct
     2521 ggcgttcgcg gacgagggca ggatattcct tgccttccac atcaatcgtc acgaggctgg
     2581 aagcagaaac cttcgacaaa gccaagccgg cggtgtgtgc atccaaaaca attgaaatcg
     2641 gttcgagatg acgaccataa atcaccgcag ggagtttccc ttcgcggcgc atggctttca
     2701 cctgcttgcc gatcacatca cgtttggttg ctttcaatac tactttttcc atcttaccta
     2761 actccttggg cgcctctcac acagcaactc cgctgtgtta ccttcgcccc aaaatgaatt
     2821 tctgtaaagc gggtcattcc cgctgaacat ccgaattatt ttttgccgaa caacatgcgc
     2881 ccgagcaaaa gcataatgcc tgcaaacaaa ccgcttacca agccggcaat aatcgcaccg
     2941 cgagtatcca ttacggtagt gacattcccc tgcacattgg gcgataatag gattagcgac
     3001 tcaatacgct cagcacggac ttcacgtcca gccacaatgc cggcataggc gttgccaaac
     3061 tcgacgcttc ctgccacgac cgcgcctgat acgccattca aggttaattc agtcgtttga
     3121 gcgactccta ctactgaatt ttgaacatta gcagtgtcgg catgcagagc cgctatgcca
     3181 gagtcggtca actttaagtt ggtgaccttc gcctgcgcta ccgcactcat ggtcatatcg
     3241 acatcttctg aagtgaccaa accagccgag ctttggtgca tgcgaaccaa ctgcgcgttg
     3301 accgtttcca cgtccacttg ggagaggttg gtcacctgcg ggttttgctc agaagaagga
     3361 tttgtctcgg tcatgatttt ggcagaaaat acgcctcctc acggaggcgc aggttgaata
     3421 acggctcaga ttttatccgt ttgagggggg ttcgtcaagg aagggcgcct atcccgcatt
     3481 tcgtgcgcaa tctcggctga aatatcatct ccgcgggctt cgaggtggct gataatgttt
     3541 gcataggact catcgtcgcg gttttggctc agtttcgagg cggcgtcgag gcgggttaca
     3601 tccaaagcaa aacctaccac cccacgcatc tctttttcaa cgaagtcggc gggcaagcct
     3661 tccatgctat agcctgtgcc ggtttcgtgc ttagagacca acttgctaag cagggaatgt
     3721 aatccctccc cttccaacat gcggaccttt ccatacacat gtacattgat gtaattccag
     3781 gttggcacat tgacatggtt ataccagcgc ggagaaatat aggtgtgcgc accctgaaaa
     3841 ataagcagaa cttcctgctc gccaaaagac ttccactgcg ggttggcgcg agacatatgc
     3901 cccagaacgg tcagagaccc atcggcattt tctataactt ccaccggtaa atgcgtggca
     3961 agtaattttt caccatcgct cgagacaatc gccggaaaac tattggcttt tagaaattcc
     4021 acaatcttat cttggtcttc ttcacgataa tattttggga tatgcatgtt aaacctctaa
     4081 ggaaagtata acaatatttc ttaagaggct caggtagaat tttgcaacat gtcgaaacgg
     4141 tataaaaaga aacggtctac tactccagcc tgggtagaca gcctcattgt cattttagaa
     4201 atccttggag ccattggcac tgtgatcgca ggcatttata gcctgcttac gtgggtgttt
     4261 tgataaacct tggttataat
//
LOCUS       DAOQJE010000193         4259 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_12557_length_4259_cov_2.611323, whole genome shotgun sequence.
ACCESSION   DAOQJE010000193 DAOQJE010000000
VERSION     DAOQJE010000193.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 4259)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4259)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4259
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12557_length_4259_cov_2.611323"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2948
                     /locus_tag="PLT08_06550"
     CDS             <1..2948
                     /locus_tag="PLT08_06550"
                     /inference="COORDINATES: protein motif:HMM:NF024883.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNE04157.1"
                     /translation="AYNSQARNYIIYGDFKKTFNLFQKALKHAELAGEKDLICSCLVR
                     FSGIANLIGKNELAIRLSQKGLELAQEIGDLEMQSHAKINLFSAFNQMGQRRYEIITD
                     ALNLALKSGSLQVIGSAYNILGTNSQDAALSIKYIKKSIEAHKAWGSIVDVLTTEANL
                     AYIYTQLGLLFFSRRLFSQLLADLPYKDLYFETIVLSNLVTIEVETKSLEQARFYLNR
                     FSETAPLLNSEHLNSYLFSAYGNVSFLAGEYSQAVQHYSKALNLARTNKFYRENEMLT
                     ELGKVYLAQRDFAQALASTTEAVKIHEEASFAQPENSSCPNQRIWWLHTQALHANHKT
                     VEMRTALERAYDFLLEGIANVPDVGLRRNYLNKVTGNRDLLRFWLSEGKREKLSKERL
                     FAYLNIKSNTREPFTRLTDTSQRLNLLKDIPAIQTFLVEEAIELSGAERLMLILEDGK
                     KLEVAESILPVGEEADKVFSSIKKHLTQARLTRTVQLVLPKKQGLSRIIAPLIAQNQV
                     VGYLYADMDSIYGLFDETDRDMLGMLANQAAVALDNAGLVTNLEQKVNERTAQLQASI
                     TETQRLLKESRTLGRVGRDISSSLDLEQVLENITSYAKDLLNADLSALLLPEQGGSTF
                     RAVTALGIYANDLLNDVVSLGDGLLGSVAKNKIGEIINDTHLDSRSIAISGTELIEHE
                     HLLAVPLLEDNELRGLMAVWRIGAGREFNQNELNFLINLSRQAVVALKNAQLFSEAHE
                     SRLVAEQANKMKSAFLANMSHELRTPLNAIINFTELVQMGVMGEVNEEQKEALGYSLG
                     SSKHLLQLINDVLDISKIQAGKLTLFLDQKVDLSNEIEDVLHMVEPLLKKQIELYEYD
                     VKLVKDVDANLPLITCDRRRIRQVLLNLLSNAVKFTQKGSITLSVKRKADHLEFAVID
                     TGTGIRQDMQTQIFEPFQQTEDGIKQVEGTGLGLPITKSLVEAHGGRIWLESELGEGS
                     AFFFILPTR"
     gene            3100..4104
                     /locus_tag="PLT08_06555"
     CDS             3100..4104
                     /locus_tag="PLT08_06555"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor domain-containing diguanylate cyclase"
                     /protein_id="HNE04158.1"
                     /translation="MPANNILELLIIMFVMVVLFYIIRSWVEQPISNETRKILNLSRD
                     GLVILNKKGEILLVNQAAERLLGETWESLQHKNITSINYEIAVAVVSAIELQKSKFAF
                     LTLNNISMYVKVYVNKSAKEWWKNHGLWWKDNCVLELEDVTEYTYSQHAPTKSQKYLY
                     DLVYIDHLTSAFNRRYFDTEIPNILMNAMSENLPISLLLLDIDKFKEINDTHGHQVGD
                     IVLKFLVESMKRNSRKEDMVFRLGGDEFALLLVNTSEKVAMARAQQLCNDIFEAKVDA
                     LNRHIKLTISIGVATFPKPAASLGQLLQFSDNALYHAKAEGRNCIRLYTTPFIVSSPE
                     "
BASE COUNT         1322 a          901 c          896 g         1140 t
ORIGIN      
        1 tggcatataa cagccaagct cgtaattaca taatatatgg tgactttaag aaaaccttta
       61 atctttttca gaaagcattg aaacacgccg aactcgccgg agaaaaggat ttaatttgtt
      121 cgtgtttggt gcgctttagc ggtatagcca acctaatcgg gaaaaacgag ttagctatta
      181 gactttctca aaaaggactc gaacttgctc aagaaatagg cgatctggaa atgcagtcgc
      241 atgccaaaat taacctattt tcagccttta atcagatggg gcagaggcgg tatgaaatta
      301 ttacagacgc tctcaacttg gcgttgaaat ctgggtctct ccaagttatt ggtagtgctt
      361 acaacattct gggaactaat tcgcaagatg ccgctttgag tattaagtat ataaaaaaat
      421 ccattgaagc gcacaaagcc tggggctcca ttgtagatgt attaacaact gaagcaaacc
      481 tggcttatat ctacactcaa ctaggtctac tctttttcag caggagatta ttttcccaac
      541 ttcttgccga tcttccatac aaggatttgt acttcgagac aatagtgctt tccaacctgg
      601 tcactattga agtagaaact aaatccctgg aacaggcacg cttttatcta aacagatttt
      661 cagaaacagc tccgctttta aatagtgaac acttaaattc ctacctgttc agcgcgtatg
      721 gcaatgtgtc gttcctggct ggagaatatt ctcaagccgt tcagcattac agcaaagcct
      781 tgaatttggc gcgtacaaac aagttttatc gagaaaacga gatgcttacc gagcttggca
      841 aggtctattt ggcacaaaga gattttgccc aggcactagc cagcacaacc gaagcagtaa
      901 aaattcatga agaagcctcc tttgcccaac ctgaaaatag tagttgtccg aaccagagaa
      961 tttggtggct ccacacccag gctttgcatg ctaatcataa gactgttgag atgcgaaccg
     1021 ccttggaacg cgcctatgac tttctcctgg aaggcattgc gaatgtccct gatgtaggat
     1081 tacgccgcaa ctacctcaac aaagtaacag gcaatcgaga tctattacgc ttctggcttt
     1141 ctgaaggtaa aagggaaaaa ttgtccaaag aacgcctgtt tgcctatctc aacatcaaat
     1201 cgaacacccg tgaacccttt acccgcctca cagataccag ccaacgctta aatcttttga
     1261 aagacattcc cgcaatccaa accttcctcg tagaagaagc catagaacta agcggtgccg
     1321 aacgattgat gttgattctc gaagatggca aaaagctcga agtggcagaa tccatcctcc
     1381 ctgttggaga agaagcggac aaggtgttct cctcgatcaa aaaacacctg acgcaagccc
     1441 gcctcacccg gaccgtacaa ctggtcctcc ctaaaaagca gggtctcagc cgcatcattg
     1501 caccactcat tgcccagaac caagttgttg gttatctcta tgccgatatg gattccatct
     1561 atggactgtt cgatgaaacc gatagagata tgctcggaat gcttgccaac caagctgccg
     1621 ttgctttgga taatgccggg ctggtgacaa acctcgaaca aaaggtcaat gaacgcaccg
     1681 cacaattaca agccagcatt accgaaactc aacgtctgct caaagagagt cgtaccttag
     1741 ggagagtcgg gcgtgatatt tcctctagcc ttgatttgga acaggtgttg gaaaacatta
     1801 cgtcttatgc caaagacctg ctaaacgcag acttgagcgc gttattgcta cctgaacaag
     1861 gaggctcaac gtttagggca gtaacggcat taggaatata tgcaaacgat ttacttaatg
     1921 atgtagtgag tctaggtgat gggcttctgg gatcagttgc taaaaataaa atcggtgaga
     1981 tcattaatga cactcacctt gatagtcggt caattgcgat cagtgggaca gaacttattg
     2041 aacatgagca tcttttagcc gtacctttgc tggaagacaa tgaattacgt ggtttgatgg
     2101 ctgtttggcg tattggtgct gggcgcgagt ttaaccaaaa tgaattgaac ttcctgataa
     2161 atttatcccg tcaggcggtg gtagcgttga agaacgcaca gctattcagc gaagcacatg
     2221 aatcaaggct ggttgctgag caggcgaaca aaatgaagag cgctttcctt gcgaacatga
     2281 gccatgaact gcgcacacct ctgaatgcca tcatcaactt taccgagttg gtacaaatgg
     2341 gtgtcatggg tgaggtcaac gaagaacaaa aagaagcact gggttattct ctcggcagtt
     2401 ccaagcactt attacaactt atcaacgatg tgctcgacat tagcaagatc caggcaggca
     2461 agctcaccct cttcctcgat caaaaagtag atttgagcaa cgagatcgag gatgtgctcc
     2521 acatggtcga gccgctcctg aaaaaacaga ttgagttgta tgaatatgat gtcaaactgg
     2581 tgaaagatgt ggatgccaac ttgcctctga tcacctgtga ccgtaggcgc atccgccaag
     2641 tgttattgaa cctgctctcg aatgcagtaa agttcacgca aaagggcagc atcaccctga
     2701 gcgtaaaacg caaggcagat caccttgaat ttgctgtcat agatactggg actggtattc
     2761 gacaagatat gcaaactcag atcttcgagc ctttccagca gactgaagat ggcatcaagc
     2821 aagtggaagg tacagggttg ggtttgccca tcaccaagag tttggttgaa gcacacggtg
     2881 gaagaatctg gctcgaaagt gagctgggtg aaggttctgc attcttcttt atactcccca
     2941 cacgataaca gtctcaattc acaattatgc ctgtccatac ggacaggcat aattatttaa
     3001 ggacaggtga cgaattccac aggcgtacat acaattgata tatttaaaca catgacttga
     3061 gttttctcaa tctaggtttc actaaaggac taataataaa tgccagcaaa taatattttg
     3121 gaactactaa ttattatgtt tgtgatggtg gtgctttttt atattattag aagttgggtt
     3181 gaacaaccaa tctcaaatga aactagaaaa atactgaatt tatcacgaga tggattagtc
     3241 atcttaaata aaaagggcga gatacttctt gtgaatcagg cagcagagcg cctgcttgga
     3301 gaaacttggg agagccttca gcacaaaaac attacctcga ttaattatga aatcgctgta
     3361 gctgtagttt cagcaatcga attgcaaaag tcaaaattcg cgtttcttac tttgaataat
     3421 attagcatgt acgttaaggt ttatgtaaat aagtctgcaa aagaatggtg gaaaaatcat
     3481 gggctttggt ggaaagataa ctgcgttttg gaattagaag atgttactga atatacctat
     3541 tcacaacatg cgccaactaa atcacaaaaa tatctatacg acttggttta cattgaccat
     3601 ttgaccagtg cattcaaccg ccgatatttt gacacagaaa taccgaatat tttaatgaat
     3661 gcaatgtctg aaaatctacc tatcagcctc ttacttctgg atattgataa attcaaagag
     3721 attaacgaca cacatgggca tcaagttggt gatattgtat tgaaattttt agtagaaagc
     3781 atgaaacgaa attcgcgcaa agaagatatg gtctttcgcc taggtggaga tgaatttgcc
     3841 ttactactgg taaacacttc tgaaaaagta gcaatggctc gtgctcaaca gctttgcaat
     3901 gatatctttg aagccaaggt tgatgcatta aacagacaca taaaactgac aatatcgatt
     3961 ggcgtagcaa cctttccaaa accagcggct tctcttggac aactccttca gttttcagat
     4021 aatgctttgt accatgcaaa agctgaaggg cgaaattgta tacgtttata taccactcca
     4081 ttcattgtct cctccccaga atgatattct ttattcatgg gttctattat tgaaacaaaa
     4141 actacccttt catttttctg tatacaagat atagcaaaaa taacaccaaa gaaggatatt
     4201 ccagcatggt acacttaatt aacaaaaaat ctttatttca aacgagaact ttcccatga
//
LOCUS       DAOQJE010000194         4252 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_12586_length_4252_cov_6.974744, whole genome shotgun sequence.
ACCESSION   DAOQJE010000194 DAOQJE010000000
VERSION     DAOQJE010000194.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 4252)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4252)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4252
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12586_length_4252_cov_6.974744"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..299
                     /locus_tag="PLT08_06560"
     CDS             <1..299
                     /locus_tag="PLT08_06560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HNE04159.1"
                     /translation="LVIYLRASVDTLLNRINHRGRDYERTIAPDYLQSLNDLYEGWID
                     NFTLCPVLAVPADDLDYVAHNGHLRLIVSKIEAKLTGKEEVVFEPEEVARAAED"
     gene            complement(352..1695)
                     /locus_tag="PLT08_06565"
     CDS             complement(352..1695)
                     /locus_tag="PLT08_06565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNE04160.1"
                     /translation="MQETKTNRPSGMFAFTIVWLGQIISVLASAMSQFGLTIWMYEKT
                     QSATALGLMQVFFITPFLIISPIAGVMVDRHNRKLMMMVSDLTAGLATILILIFQSLG
                     VLQFWHLYVASIIYGLGNAFQWPAYSAAISTMVPKDQYGRANGMMSLIEAGPGVFAPL
                     LAGALLPLIGLTGILVIDVVTFSFAVGALLIVHIPQPERTVEGVQGKGSVWQEAVFGF
                     KYIFARPSLLGLQMVFFFGNLFSGILFTLLAPMILARTDNNSLMFGTVQTAGAIAGVV
                     GGLIMSAWGGFKRKVHGVLGGWAITGLGSAVIGLSFGLPAWVIGVAIASLISPLVNAS
                     NQAIWQSKVAPDLQGRVFSARRLIAWFTNPISPIVAGTLADYVLEPQMRAGGTLSQTF
                     SWLVGTGPGSGMGLIILACGVFCVLIGVAGYFIPAIYNAETILPDHDQLAKVEPA"
     gene            complement(1847..2233)
                     /locus_tag="PLT08_06570"
     CDS             complement(1847..2233)
                     /locus_tag="PLT08_06570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE04161.1"
                     /translation="MANVEERMKILKLIEEGKISAEEGAKLLSALNDSRKNIPTPPRP
                     PSAPGGARMLRVRVTDTRTGRSKASVQIPLALVDAGLKIGAHFAPEVQGVDMSNVMEA
                     LRMGVTGKIIDVTDEEDGEHVEIFVE"
     gene            complement(2274..3566)
                     /locus_tag="PLT08_06575"
     CDS             complement(2274..3566)
                     /locus_tag="PLT08_06575"
                     /inference="COORDINATES: protein motif:HMM:NF015185.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNE04162.1"
                     /translation="METIKKQPLLSGILILFLTAMIFANIGGNMYEGLMPLYLKELKA
                     DITDIGLFFTLAQIVPLFLQILGGWVSDSLGRLRAIAIGSVFGLLGFIPLVLADTWHW
                     LLIATAVGAIARALVGPSFDAFIAEHSTEENRGKVYGISQAIFMIVTVVGPPLGGWLT
                     GAYGFKLMLFIAGVFYAIAVAMRLSMAREATKGEKENGEKKSLSFTSLKENLGSMIAL
                     IFSGGLITWMLITDGLRDTSFQFSGNLFPVYMQEIGGLSYQQIGWVTSMFGIFMMLST
                     IPGGWLSDKAGERVGIAAGMFLMSVALFILIVIPQGTQWQWLFFVGWAVAGLGAGISG
                     PAYQSLISKAVPQKLRGLAFGLFSTSLGIVSLPAPWIGAQLWARFGPTAPFKITATVI
                     LLSIIPIWLKFKLPKTTEETQPEAEQPAEENNVAIAAG"
     gene            complement(3573..4235)
                     /locus_tag="PLT08_06580"
     CDS             complement(3573..4235)
                     /locus_tag="PLT08_06580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE04163.1"
                     /translation="MSSEERRKILQMVADGKISAEEAATLMRALDDSNAEEASAEMEI
                     IEVRPASSAEGAGSSYQEKSHAPELDAVRMRAHRFSGAFLWIGTIVTVLIAWWMFNVQ
                     QNSGLNFWFFCLSLPLTLGILLIALGAGSRTSRWLYVNVDRSQSKDQDGPRKISLAFP
                     LPLGFASWFLRTVGRHIEELRDKNVEAIADTIALAKNMTEPLIVHVDDDSDGTQVQVF
                     IG"
BASE COUNT         1261 a         1181 c          863 g          947 t
ORIGIN      
        1 acctcgttat ttacctccgc gcctctgtag ataccctcct aaaccgcatc aaccatcgcg
       61 ggcgtgacta tgaacgcacc atcgctcccg actatctgca aagcctgaat gacctctacg
      121 aaggttggat cgacaacttc accctctgcc ccgtcctcgc cgtcccagcc gacgatctgg
      181 attatgttgc ccataacggt cacctacgct tgattgtctc aaagatcgaa gccaaactca
      241 ccggcaaaga agaagttgtc ttcgaacctg aagaagtagc ccgcgcagca gaagattaga
      301 tagaacaaaa agcggagttg agatatctca actccgcttt tttattttct gttatgcagg
      361 ttctactttt gccagttgat catgatcagg caagatcgtc tctgcattgt aaattgcagg
      421 gatgaaatac cccgctacgc cgatcaagac gcaaaacaca ccgcatgcca aaatgatcag
      481 ccccatccct gaaccgggac cggtgcccac caaccagcta aaggtctgcg aaagcgtccc
      541 accggctcgc atttgcggct caaggacata atccgccagt gttccagcaa caataggaga
      601 aatgggattt gtgaaccaag caatcaaacg ccgtgcggaa aaaacacgtc cttgaagatc
      661 tggtgccacc tttgattgcc aaatagcctg attagatgca ttcacaagcg gactaatcaa
      721 cgaagcaata gcaacgccaa tcacccatgc aggcagacca aaactcaagc caatcacagc
      781 agacccaagc cctgtaattg cccatccacc taacacgcca tgtaccttgc gtttaaaccc
      841 gccccaggca ctcataatca agccacccac tacaccagca atcgctcctg ccgtttgaac
      901 cgtaccaaac attaagctgt tgttatcggt tcttgcaagg atcatgggcg ctaataaagt
      961 gaacagaata cccgaaaata aatttccaaa gaaaaacacc atctgcaaac caagcaggct
     1021 ggggcgggca aaaatatatt tgaaaccaaa aaccgcctcc tgccaaacac tgccttttcc
     1081 ctgaacacct tccaccgttc gttcaggttg agggatgtgg acgatcaaca aggcgccaac
     1141 ggcaaatgaa aatgtcacaa catcaataac aaggatgcca gtcaaaccga tcaatggcag
     1201 caacgccccg gctaatagcg gggcaaaaac gccgggtccc gcctcgatca gggacatcat
     1261 accgtttgcg cgaccatact gatccttcgg caccatcgtg gaaattgcag ccgaatatgc
     1321 gggccattgg aaagcatttc ccaacccgta aatgatcgaa gctacataca aatgccagaa
     1381 ttgcaaaaca cccaacgact gaaagattaa aatcaaaatc gtagcaagtc cagcggttaa
     1441 gtcgctaacc atcatcatca acttacggtt gtgccggtct accatcacac cagctatggg
     1501 agaaataatc aagaatgggg taatgaagaa gacttgcatc aaacccagtg cagtagcgct
     1561 ttgggtcttt tcgtacatcc agatcgtaag cccaaattga ctcatcgcac tggcaagcac
     1621 ggaaatgatc tgccccagcc acacaatcgt aaatgcgaac ataccactgg ggcgatttgt
     1681 ctttgtctct tgcataaaag tatcctttga tgatctgtga taaaaatgat acaacgcgca
     1741 agagaaatct cttgcgcgtt gatggctata acatcaagcg atggacaagg tatagtgatg
     1801 ggaacatagt tttctccttc tgataaccaa cacgaaccga cagactttat tcgacaaaga
     1861 tctcgacgtg ttcaccatct tcttcatcgg tcacgtcaat gatcttgccg gtaacaccca
     1921 tgcggagtgc ttccataaca ttactcatgt ctacaccctg aacttcagga gcaaaatgag
     1981 caccaatctt caacccggca tccaccaacg ccagtggaat ttgaacagag gctttggaac
     2041 gcccagtgcg ggtatctgtc acgcggactc tcaacatgcg tgcaccaccg ggagcagaag
     2101 gcgggcgcgg aggcgtgggg atattcttcc gcgagtcgtt cagagctgag agcaacttag
     2161 cgccctcctc cgcactgatt ttgccttcct caattaattt cagaattttc attctttctt
     2221 ctacgtttgc catgttccat ttctcctttc gccgttcagg acgaacgctt attttatcct
     2281 gccgcgatag cgacgttatt ttcttctgct ggctgctctg cttcgggctg ggtttcttca
     2341 gttgtttttg gaagtttgaa ttttaaccaa attgggatga tcgaaagcaa aataacagta
     2401 gcagtaatct tgaagggagc cgtcggacca aaacgagccc acaactgagc cccaatccac
     2461 ggagccggaa gcgatacgat gcccaaactg gtgctaaaca gcccgaaagc aagtccgcgc
     2521 aatttttgtg gaaccgcttt actgatcaaa gattgatacg ccggaccgga aatccctgcc
     2581 cccaagccag ccacagccca ccccacaaag aataaccact gccactgcgt cccttgaggg
     2641 atgacaatca agatgaacaa agctacagac atcaagaaca tgccagccgc aatccccacc
     2701 cgctcccctg ccttatcgga aagccaaccg ccagggatgg tgctcagcat cataaaaata
     2761 ccgaacatcg aagttaccca gccgatctgt tgataagaca agccaccgat ctcttgcata
     2821 taaaccggga acaaatttcc agagaattga aaagacgtat cgcgtaaacc atcggtaatc
     2881 aacatccaag tgatcaagcc gccagagaaa atcaacgcaa tcatagagcc caagttttcc
     2941 ttcaagctcg taaaggaaag actcttcttt tcgccattct ctttttcgcc ttttgtcgcc
     3001 tcgcgcgcca tgctcaaacg cattgctacc gcaatggcat aaaacacacc tgcaatgaat
     3061 aacatcaatt tgaaaccata ggcgccggtc aaccagccac ctaaaggagg tccgaccacg
     3121 gtaacgatca taaagatcgc ctgcgaaata ccatacacct tgccacggtt ttcttctgtg
     3181 gagtgttccg caatgaaagc gtcaaagctg ggtccgacca aagcacgagc aatagcacct
     3241 acagcagtag caatcaacaa ccagtgccag gtatctgcca acaccaacgg aataaaccct
     3301 aaaagaccaa acacactacc aatcgcaatg gcacgtaaac gtcctaaaga atctgaaacc
     3361 caaccaccta aaatctgaag gaacaaaggc acaatctgag ccaatgtaaa gaacagaccg
     3421 atatcggtaa tatctgcttt cagttccttc aaatacaagg gcatcagccc ttcatacata
     3481 tttccgccaa tattcgcaaa gatcattgcc gttagaaaca agatcaatat gccacttaat
     3541 aaaggttgtt tcttgatggt ttccattttt ttctatccta taaaaacttg cacctgcgta
     3601 ccatcactgt catcgtccac atgtacgatc aacggttccg tcatattctt agctaaggca
     3661 atggtgtcgg caatggcttc tacattctta tcgcgtagct cttcaatatg tcgtcccact
     3721 gttcgcaaaa accaacttgc aaaaccaagt ggcaaaggga atgccaatga aatcttgcgc
     3781 ggaccatctt ggtcctttga ctgcgaacga tctacattca catataacca acgcgatgta
     3841 cggctgcccg cacccaaggc gatcaataaa ataccaaggg tcaatggcaa gcttagacag
     3901 aagaaccaaa aattcaaccc agaattttgc tgaacattga acatccacca cgcgatcaaa
     3961 accgttacga tcgttccgat ccaaaggaaa gcgccagaga accgatgagc gcgcatacga
     4021 acagcatcta attcaggggc atgactcttt tcttgatatg aagacccggc tccttccgcg
     4081 ctcgaagcgg ggcgcacttc gataatctcc atctccgctg aagcctcttc agcgttagag
     4141 tcgtccaagg cacgcatcaa ggtagcggct tcttccgcac tgatttttcc gtctgcaacc
     4201 atttgcagaa tttttctgcg ttcttcagaa gacattagga acctccttca ag
//
LOCUS       DAOQJE010000195         4249 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_12595_length_4249_cov_6.467573, whole genome shotgun sequence.
ACCESSION   DAOQJE010000195 DAOQJE010000000
VERSION     DAOQJE010000195.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 4249)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4249)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4249
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12595_length_4249_cov_6.467573"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(143..991)
                     /locus_tag="PLT08_06585"
     CDS             complement(143..991)
                     /locus_tag="PLT08_06585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE04164.1"
                     /translation="MINWQELTIDMFVEQLRTSYRRTYGDIQSEYGNIVSWVGRLALE
                     NISNSDALYHDIDHTIMVSLAGLAIIEGKHLREGGVSARDWMRYMIAVLCHDIGYVKG
                     VCKNDTDELFATGEGTERIKISKDGTDVALTPYHVNRSKLFVMERFGSGLLKDMEEHL
                     NAELITTYIEMTRFPSPKGEFYQDTQGLGGLVRAADFVGQLGDPDYFRKTPALFYEFQ
                     ELGANEKLGYQSPNDMRKNYAKFYWEQVNPYLKDALVYLRLTEDGKQWIANMHSHVFD
                     SEHTYK"
     gene            1078..1371
                     /locus_tag="PLT08_06590"
     CDS             1078..1371
                     /locus_tag="PLT08_06590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE04165.1"
                     /translation="MTKRRIFLNWFLIGLSIFLLLNILASALSSDCGLPALFGLGGCA
                     DAISRIGFPFVFFEQGGFAYHSSFNLPVFIIDIAIGIGFAVFLGSYKSRKSNS"
     gene            1372..2292
                     /gene="miaA"
                     /locus_tag="PLT08_06595"
     CDS             1372..2292
                     /gene="miaA"
                     /locus_tag="PLT08_06595"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0052381 - tRNA dimethylallyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HNE04166.1"
                     /translation="MTKPPVILIVGPTAVGKTELALRLAEQMNGEIVSVDSRLFYRGM
                     DIGTAKPSKEEMARVPHHLIDIVNPDETLSLAVFQEMAQTIIEGIHARGRLPFLVGGT
                     GQYIRAVTQGWSPPEVTPNEQMRAVLETLQEEKGKEWLHAKLQILDPEAAQAIDLRNV
                     RRTIRALEVIFSTGRRFSEQRGIGDSPYHLITIGLARPREELYQRVDERIEKMFADGL
                     LNEVKSLLDQGYSPTLPSMSAIGYRECVSVVQGSMTEEQAKVEMRRLTRVFVRRQGNW
                     FKESDPSIHWFHPARENLLNEIEAMIRNSI"
     gene            2394..4094
                     /locus_tag="PLT08_06600"
     CDS             2394..4094
                     /locus_tag="PLT08_06600"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid--CoA ligase"
                     /protein_id="HNE04167.1"
                     /translation="MSNKPWLAHYDKGVPQTIEYPKAPLFHFLEEAARKYPDRACTIF
                     KGAVISYREMNERSNAVAAALVEMGVKKGDRVGIFMPNLPQFVSVYFGILKAGGVVIA
                     VNPTYPVEEILTPVNDAGIEIMFTLSRFYGKVKEVREKSGLKKIIVSNLKEALPPFTR
                     LLFTLLKEKKDGDKLDSLDSRDVWLTDLLKKYAGASKPNVDIQPDDTALFQYSGGTTG
                     VPKGAVAMHRNVVANTLQIKSWMTGLDEGKEVVLMGIPLFHVYGMVAGMNFAMATGSS
                     MVMIPNARDLKDVLENIAKYKATIFPGVPLLYNGINNHPDVKAGKYDLSSIKACISGS
                     AALMRETKEEFERLTGGKVFEGFGMSEAPTAVTCNPLNGVNKTGSIGMPLPDVDMKII
                     SLDDGETEMQLGEIGEIVVNGPQVMKGYHNMPTETANSLRQLKDGKTWLFTGDIARMD
                     EDGYFYIVDRKKELIKPGGFQVWPREVEEAIMDNPKVLEVGVGGIPDPKSGETVKAWV
                     VLKPGESMTEDELKEFCKERLAPYKVPRYVEFRTELPKTTVGKILRRELVRQHKEGGK
                     "
BASE COUNT         1126 a         1026 c         1057 g         1040 t
ORIGIN      
        1 atctggagca tgggcggcat tcggtacaaa gcaacaggca actcgcgttt atatcgcatt
       61 tgcccgaatt gcagtcaacg cagttggcag acgatttcaa agcgaaaata aaaaagcgcc
      121 gcagagaacc tgcggcgctt ttttatttat atgtatgttc cgagtcgaac acgtgggagt
      181 gcatgttggc gatccactgc ttgccatctt ccgtgaggcg caggtacacc aaggcatctt
      241 tcaaatacgg gttgacctgc tcccagtaga acttggcata attcttgcgc atgtcgttcg
      301 gagattgata gccaagcttt tcattcgcgc ctaattcttg aaattcatag aacaacgccg
      361 gagtcttgcg aaaataatct gggtctccga gctgaccgac aaaatctgcc gctcgaacca
      421 acccgcccaa accttgggtg tcttgataaa actcaccctt cggtgaaggg aagcgtgtca
      481 tctcaatata cgttgtgatc aattcagcgt tgagatgttc ttccatatct ttaagtagcc
      541 cagacccaaa gcgttccatc acgaacaact tactgcggtt cacgtggtaa ggggtcaacg
      601 ccacatcggt gccatctttt gaaatcttaa ttctttccgt cccctccccc gttgcaaaca
      661 gttcatcggt gtcgttcttg cacacaccct ttacatagcc aatatcatgg catagcaccg
      721 cgatcatata acgcatccaa tcgcgcgcac tcactccgcc ttcgcgcaaa tgcttccctt
      781 caataatggc aagcccggca agagaaacca taatggtatg gtcgatatca tgatacagcg
      841 catctgagtt cgaaatgttt tccaacgcaa gccgccccac ccaagaaaca atattgccat
      901 actccgattg aatatcgcca taggtgcggc gatacgaagt acgcaactgc tccacgaaca
      961 tatcaatggt caattcctgc cagttgatca taaagacatc ctataagggt aaaattgaat
     1021 agtgccaaaa gtttaccaga aacatacaaa aagaaaacag acggaaatcg aattgttatg
     1081 acgaaacgcc ggatctttct caactggttc ctgatcggac tctcaatctt tcttctactc
     1141 aatatccttg cctcggctct ttcgtctgac tgtggcttgc cagccctctt tgggctgggt
     1201 ggctgcgccg acgccatttc acgcatcggt ttcccgttcg tgttctttga acaaggcggc
     1261 ttcgcctatc atagcagttt caatctacct gtttttataa ttgatattgc cattggaatt
     1321 gggttcgctg tatttcttgg ctcctacaaa agtagaaaat caaattcctg aatgaccaaa
     1381 ccacccgtca tcctcatcgt tggtcccaca gctgttggga aaacggaact tgccttgcgc
     1441 cttgccgagc aaatgaacgg cgagatcgtc tcggttgatt cgcgcctgtt ctatcgtggc
     1501 atggacattg gtacggctaa gccgtcaaaa gaagagatgg cacgtgtgcc tcatcacctg
     1561 atcgacatcg ttaatcccga tgaaaccttg agtttggcgg tctttcaaga gatggcgcaa
     1621 accattattg aaggcatcca cgcccgcgga cgattgccat tcctcgttgg tggcacaggg
     1681 caatacatcc gtgccgtgac tcaaggttgg agtccgccag aggtgacgcc aaatgaacag
     1741 atgcgcgccg ttcttgaaac cctgcaagag gaaaaaggga aagaatggct acacgctaaa
     1801 cttcaaatcc tcgaccctga agctgctcaa gccattgact tgcgcaacgt ccgccgcacg
     1861 atacgggctt tggaggtcat cttcagcaca gggcgaagat tctctgaaca acgtggcatt
     1921 ggagactcgc cgtatcattt aattacgatt gggctggcac gtccgcgcga ggagttgtat
     1981 caacgggtag acgaacgcat tgagaaaatg ttcgcagatg gcttactgaa tgaagtaaaa
     2041 agcctgcttg atcagggata ctcccccaca ttgccaagca tgtctgcgat cgggtaccga
     2101 gaatgtgtca gcgtggtaca aggctccatg acggaggaac aagccaaagt cgaaatgagg
     2161 cgtttgactc gcgtctttgt gcgccggcag ggaaactggt tcaaagagtc agacccatcg
     2221 attcattggt ttcatcctgc ccgtgaaaat ttactcaatg aaattgaagc aatgattcgt
     2281 aactctatat aaatacaggg gttgtaattg acttttaata tcttcacgta tatacttcaa
     2341 acctaggtag agagtcatct tcagttatcc aacaaaccaa tggaggagga agtatgagca
     2401 acaaaccgtg gttggcgcac tatgataaag gtgtgccaca aacaatcgaa tatcccaaag
     2461 cgccgttgtt tcactttttg gaagaagccg cgcgcaaata cccagaccgt gcctgtacga
     2521 tatttaaagg cgcggtcatt tcgtatcgtg agatgaacga aagatccaat gctgtggcgg
     2581 ctgccttggt tgagatgggt gtcaagaaag gggatcgggt tggtattttt atgcccaacc
     2641 tgccgcagtt cgtaagcgtg tattttggta ttctcaaagc aggtggtgtg gtgatcgctg
     2701 tgaatcccac atacccggta gaagagattc tcaccccggt aaatgatgcc gggatcgaga
     2761 tcatgttcac gctttcacgt ttttatggca aggttaaaga agtacgcgaa aagtcggggc
     2821 ttaagaaaat cattgtttcc aatttgaaag aagctttgcc gcctttcacc cgtctgttgt
     2881 tcactcttct aaaagaaaag aaggatggcg acaaactgga ctcgctcgat agtcgcgatg
     2941 tatggctgac agatttgctg aagaaatatg ccggcgcctc aaaaccgaac gtcgatattc
     3001 agccagacga tacggcactc ttccaatatt ctggcggaac gaccggtgtg cccaagggcg
     3061 ctgttgccat gcaccgtaat gtagtggcaa atacgctgca aatcaaaagc tggatgaccg
     3121 ggcttgatga aggcaaagaa gtggtcttga tgggcattcc gctcttccat gtatatggta
     3181 tggtggcggg catgaacttt gccatggcga ccggttccag catggtcatg atcccgaatg
     3241 cgcgtgactt aaaagatgta ttagagaata tcgccaaata caaggcgacc atcttccccg
     3301 gtgtgccact tttgtataac ggcatcaaca atcacccgga tgtgaaagct ggcaagtatg
     3361 acttgtcctc catcaaggct tgtatctctg gttctgcggc attgatgcgt gagaccaaag
     3421 aagaatttga acgcttgacc ggtggtaagg tattcgaagg gttcggcatg tctgaagccc
     3481 ccaccgctgt gacttgtaac ccgctcaacg gcgtgaacaa gaccggctct attggtatgc
     3541 ctttgcccga tgtagatatg aagatcatca gtttggacga tggcgaaact gaaatgcaat
     3601 tgggcgagat cggcgaaatc gttgtgaatg gtccgcaagt gatgaagggc tatcacaaca
     3661 tgccgaccga gaccgccaac tcgctgcgtc aactgaagga tggaaagacc tggctcttca
     3721 ccggcgatat cgctcgcatg gatgaagatg gctacttcta tattgttgat cgcaagaagg
     3781 aactcattaa accgggcggc ttccaagtat ggccccgtga agtggaagaa gccattatgg
     3841 ataaccccaa ggttctcgaa gtgggcgtgg gcggtatccc ggacccgaag agcggtgaaa
     3901 cggtcaaagc ctgggtggtg ctcaaacccg gtgaaagcat gaccgaagat gaattgaagg
     3961 aattctgcaa agagcgcctg gcaccctaca aagtgccgcg ctatgtagaa ttccgcaccg
     4021 agttgcccaa gacaaccgtt ggcaagatct tacgtcgcga gttggtccgc cagcataaag
     4081 aaggcggcaa gtagttcgta attaataaaa taaaagagcc tctcaaattg agaggctctt
     4141 tttgtatgac agatatttat gcggtcaact gcggggtgcc gctcttcttg ccaagcggag
     4201 gcgggaagat ctgctcaaac tcttcgcggg tgatggattc cacttcgag
//
LOCUS       DAOQJE010000196         4240 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_12634_length_4240_cov_6.400239, whole genome shotgun sequence.
ACCESSION   DAOQJE010000196 DAOQJE010000000
VERSION     DAOQJE010000196.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 4240)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4240)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4240
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12634_length_4240_cov_6.400239"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..410)
                     /locus_tag="PLT08_06605"
     CDS             complement(<1..410)
                     /locus_tag="PLT08_06605"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HNE04168.1"
                     /translation="MSAAETLERIAHEVSVCTKCALHESRKKAVTGEGPVDAEIMFIG
                     EGPGFHENEQGRPFVGASGKFLDQLLEQAGLTRSDVFIANVVKCRPPANRDPQPEELM
                     ACGEYLDGQIEAINPSIIVTLGRISMGRYFPGAK"
     gene            complement(420..878)
                     /gene="rimI"
                     /locus_tag="PLT08_06610"
     CDS             complement(420..878)
                     /gene="rimI"
                     /locus_tag="PLT08_06610"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: protein motif:HMM:TIGR01575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008999 -
                     peptide-alanine-alpha-N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HNE04169.1"
                     /translation="MAEVLIRKMIHADLEQVIEIDQISFSLPWPKRSFQYELDENFTS
                     RCWVADVDGRIAAMMVGWMIIDELHVATIATHPDYRGQGIGKKVLVHALLAARKEGVI
                     RAFLEVRQTNEAALNMYRSLGFVEDGRRKEYYKDNNEDAILMSLNDMTHL"
     gene            complement(884..1549)
                     /gene="tsaB"
                     /locus_tag="PLT08_06615"
     CDS             complement(884..1549)
                     /gene="tsaB"
                     /locus_tag="PLT08_06615"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: protein motif:HMM:TIGR03725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HNE04170.1"
                     /translation="MLLAVDTSTALVGLALYDGDQVLGEMTWTTKQHHTTELSPALSG
                     LLKRCGVTMTSVNALGVAIGPGSFTSLRVGLSLVKGIALARSLPVMGISTLDVIAAAQ
                     PISETPLIAVLQAGRTRVAYGVYQSVDGQWQAEGPVRSGTLDEMLSQFESATLIAGEL
                     TSEERKKVSKAKHFQLASPVLCVRRPAVLAELAWARWQDDDADDAVSLAPIYLHVAGT
                     PIQ"
     gene            complement(1552..2046)
                     /gene="tsaE"
                     /locus_tag="PLT08_06620"
     CDS             complement(1552..2046)
                     /gene="tsaE"
                     /locus_tag="PLT08_06620"
                     /inference="COORDINATES: protein motif:HMM:TIGR00150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HNE04171.1"
                     /translation="MSILDAHMLEFFSRSPEQTRRIGMRLGSLLEAGDVICLQGNLGA
                     GKTTFTQGLAQGWGSLDTVSSPTFILVNQYRRADGAQLFHLDAYRLETVPEAEELDLD
                     SMLTEGALIIEWPERLGDLIPAEHLHIRLEHLSEEHRQMNFHAHGKRYDDLLDSIRQS
                     MVGG"
     gene            complement(2124..3446)
                     /locus_tag="PLT08_06625"
     CDS             complement(2124..3446)
                     /locus_tag="PLT08_06625"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNE04172.1"
                     /translation="MDPQNTDLPIGSLRIGLFTDTYAPQVNGVSVSLQMISEGLKKRG
                     HQVTIFAPKFPGYKDNEPNVMRLPSLKYLNNPPIYVAVLGTPRSTWKLTRKHFDVLHA
                     HSPASVGLLAYLTASTKRLPLIYTYHTSITDYTHYIKFIGNTGIIKYAASWFSKASTD
                     LGDQIIVPSPKFYRLLLNQKVKQPISIIPNGIDLSMFKVAKNPGALRKRLGLGADVPI
                     MLTVGRMDPEKRLDFIVEAFDLIADRVPNAHLVFAGDGGARKHVEEKANSTRAKDRIH
                     FLGMVNRADLPDILHDANVFLSASTTEVHPISVIEAIASGLPFVAVQDEAFEGMLEEG
                     QNGYTVPLDVKKYADVLADLLPDRERLQRFSAHSRELSEKYSIEAQVKALEKLYMEAI
                     LQNWRGTLFKRMIPRQLNEIPQRISKEINEFPQKITKEINKILPGKKD"
     gene            complement(3470..3697)
                     /locus_tag="PLT08_06630"
     CDS             complement(3470..3697)
                     /locus_tag="PLT08_06630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF951 domain-containing protein"
                     /protein_id="HNE04173.1"
                     /translation="MLPDLQMNDHLRLRKPHPCGSYEWTVVRLGADIGLECKGCQHRV
                     MLTRRELTKRMKTNLSQQEREKHRDSEEHNT"
     gene            3924..>4240
                     /locus_tag="PLT08_06635"
     CDS             3924..>4240
                     /locus_tag="PLT08_06635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HNE04174.1"
                     /translation="MNKDFTVKFWGVRGSYPTPGASTVQYGGNTACVEVNAGGRTIIL
                     DAGTGIIPLGRELAKRQNLELLILFSHLHHDHTQGFPFFVPAYMPKAKLHIYGPGGTA
                     ETV"
BASE COUNT         1073 a         1092 c         1000 g         1075 t
ORIGIN      
        1 atctttgcgc cgggaaaata tcgccccatg gaaatacgcc ccaacgtcac aatgatactc
       61 gggtttatcg cttcgatctg accatccaaa tattcaccac atgccattaa ttcttcaggt
      121 tgcgggtcac ggttggcagg cggacggcac ttgaccacat tcgcaatgaa cacatccgaa
      181 cgagtcaaac cggcttgttc caacaattga tctaaaaact tacccgatgc ccccacgaac
      241 ggacgtccct gttcattctc atgaaagccc ggaccctccc caatgaacat gatctccgca
      301 tctaccggac cttcacccgt cactgctttc ttgcgcgact catgcaaagc acacttcgta
      361 caaacagaaa cttcatgggc aattcgttca agggtttcag cggcagacat gcagtctcct
      421 tacagatgcg tcatatcgtt caacgacatc agaatggcat cctcattatt atctttgtaa
      481 tactcttttc ttcgaccatc ttccacaaag cctaaacttc gatacatgtt caaagccgct
      541 tcatttgttt ggcgcacttc aagaaaggcg cggatcaccc cttcttttct cgccgccaaa
      601 agggcatgaa caagcacctt ctttccaatt ccctgtccac gatagtcggg atgtgtagca
      661 attgttgcga catgcaattc atcaatgatc atccagccca ccatcattgc agcaatacga
      721 ccatccacgt cagccaccca acaacgcgac gtaaagtttt catctaattc gtattgaaag
      781 gaacgcttcg gccacgggag tgaaaaagaa atctgatcga tctcgatcac ctgttccaaa
      841 tctgcatgga tcatcttacg aattagaact tcagccataa tctttactgg atcggtgtgc
      901 ctgctacatg caagtaaatc ggcgcaagcg acacggcatc atcagcgtca tcatcctgcc
      961 aacgcgccca agctaactct gccaatacag ccggtctgcg cacacacaaa actggagacg
     1021 ccaactggaa atgttttgcc ttggagactt tctttctctc ttcggatgtt aactccccag
     1081 cgatcaaagt cgcactctcg aactgactca acatctcatc cagggttccg cttcttacgg
     1141 gaccctcagc ctgccactga ccatccacac tttgatacac cccgtaagcg actcgcgtcc
     1201 gcccggcttg taaaacagct attagcgggg tttctgaaat gggttgtgct gcggcaatca
     1261 catccaaggt ggagattccc ataacgggta ggcttcgcgc caatgcaatg cccttcacca
     1321 aagacaaccc gactctcaaa ctcgtgaatg aaccgggtcc gattgctaca cccaaagcgt
     1381 tgaccgaagt catggtcacg ccgcagcgtt tcaaaagtcc ggaaagagcc ggggaaagtt
     1441 ccgtggtgtg atgctgtttg gtggtccacg tcatctcacc gaggacttga tccccatcat
     1501 acaaagctaa cccaaccaat gcggtggaag tatctacggc aagcaacata gttaacctcc
     1561 caccatagac tgacgaatgg agtctaagag atcatcgtag cgtttgccgt gcgcgtgaaa
     1621 attcatttga cggtgttctt ctgaaaggtg ctcaagccga atatgcaaat gctcagctgg
     1681 gatgagatcg cccaagcgtt cgggccactc aatgatgagc gcgccttcag tgagcatgga
     1741 gtcgaggtcg agctcctcag cttcgggcac ggtttcgagc cgataggcgt cgaggtgaaa
     1801 aagttgagcg ccatcggcac ggcgatattg attgaccaga ataaacgtgg ggctagaaac
     1861 ggtatctaac gacccccaac cttgtgccag accttgtgtg aaggtggtct tgcctgcgcc
     1921 aaggttgcct tgtaaacaaa taacatctcc cgcctcaagc aaactgccaa ggcgcatgcc
     1981 gattcttctt gtttgttcgg ggcttctgct gaaaaactcc agcatgtggg catcaaggat
     2041 ggacatcggg ttggaattat accccccgct ttgctagtta caaattacgg gttacgcatt
     2101 gtggatacgt aacccgtaat cattcagtct tttttccctg gcaggatctt attaatttcc
     2161 ttggtgatct tttgcggaaa ttcattgatc tctttcgaaa ttctttgagg aatttcgttt
     2221 aattgtctgg gaatcatgcg tttgaagagc gttccgcgcc aattttgaag gatggcttcc
     2281 atatacagtt tttccaaggc tttgacctgc gcttcgatgg aatacttttc gctcagttcg
     2341 cgagagtgtg cgctgaaacg ctgtaaccgt tcacggtctg ggagcaagtc ggcaagtaca
     2401 tcagcgtatt tcttcacgtc gagcggaacc gtatagccgt tttgcccttc ttcaagcata
     2461 ccttcaaagg cttcatcctg cacggcgacg aatggcaaac cggaggcgat tgcttcaata
     2521 acagaaatag gatgaacctc tgttgtagac gcggagagga acacattggc atcatgcaaa
     2581 atatcgggta agtctgcgcg gttgaccata ccaaggaagt ggatacggtc tttggcgcga
     2641 gtggaatttg ctttttcctc cacatgcttg cgggcgccac catccccggc aaagacgagg
     2701 tgagcgtttg gcacacggtc tgcaatcaag tcaaaagctt cgacgatgaa atcaaggcgt
     2761 ttctctgggt ccatgcgacc aacggtgagc ataatcggca catcggcacc taaccccagt
     2821 ctctttctca atgcgccggg gtttttcgcg accttaaaca tgctcaggtc aattccattt
     2881 ggaataatac tgataggttg tttcactttt tgattcaaaa gtaaacgata gaacttcgga
     2941 gaagggacga tgatctgatc ccccaagtct gtagatgcct tactgaacca tgatgccgca
     3001 tatttgataa tgcctgtatt cccgatgaac ttgatgtaat gagtgtaatc agtaatagac
     3061 gtgtgatacg tgtagatcaa tggcagtcgc tttgtggaag ctgtgagata cgccagtagc
     3121 cccacacttg ccgggctgtg agcatgcagc acgtcgaaat gcttgcgcgt taacttccac
     3181 gtagagcgcg gagttcccaa taccgccacg taaataggcg gattgttcaa atatttcagc
     3241 gaaggcaggc gcatcacatt cggttcattg tctttatagc cggggaattt tggcgcaaag
     3301 atggtgactt gatgtccgcg cttcttcaag ccttctgaga tcatctgcaa cgagaccgaa
     3361 acaccattta cttgtggagc gtaggtatcg gtaaataagc caatgcgaag tgaaccaatg
     3421 gggagatctg tattctgtgg gtccatgtat gctccttgtt tgtatttatc tacgtattat
     3481 gttcttctga gtctctatgt ttttcccgtt cctgttgaga caagttcgtt ttcatgcgtt
     3541 ttgtcagttc gcggcgggtg agcatgacac gatgctggca gcctttgcat tccaaaccga
     3601 tatctgcgcc gagccgcacc acggtccatt cgtaagagcc gcagggatgt ggtttgcgca
     3661 aacgtaggtg atcgttcatt tgcaggtctg gaagcataca aaggattata cctttacgac
     3721 catagacgat agaccatgaa aaaccgacca tcgtctatgg tcaattgtcc atcgtccgca
     3781 cttgggaaat agttctcacc tgcccagaga acactttcct tgaaggatac tgagtttgga
     3841 acataataca agtgtcttgg aggtgacaaa gcttcagaaa gaagaccgtc atctccaaga
     3901 acctcaacaa attggaggca gacatgaaca aagatttcac agtcaaattc tggggcgtgc
     3961 gcggaagtta cccgacgccg ggtgcaagca ccgtccaata tggcggtaac acggcttgtg
     4021 tcgaagtcaa tgcaggcgga cgcacgatca ttctcgatgc gggcacgggc attattccgc
     4081 ttgggcgtga gcttgccaaa aggcagaatc ttgaattgct gattctgttc agtcatctgc
     4141 atcacgacca cacgcaggga tttccgttct tcgtgcctgc gtacatgcca aaagcaaaat
     4201 tgcacattta tggtcccggc ggcacggctg agacggtgaa
//
LOCUS       DAOQJE010000197         4240 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_12635_length_4240_cov_5.381601, whole genome shotgun sequence.
ACCESSION   DAOQJE010000197 DAOQJE010000000
VERSION     DAOQJE010000197.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 4240)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4240)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4240
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12635_length_4240_cov_5.381601"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..431
                     /locus_tag="PLT08_06640"
     CDS             <1..431
                     /locus_tag="PLT08_06640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE04175.1"
                     /translation="SSRMARLGGEFAMLMLVTVSDAEFQNLDKDFQEMRGEGYQVTLM
                     QTEDVSKKYAGWLPYEIEVTGADHEGIIQQISHHLASQGINIESMDTSATPAPMSGTP
                     LFMMKGIVLVPPKLNFNAWSDALEEVGDTLNVSVKIEVVK"
     gene            complement(483..>1501)
                     /locus_tag="PLT08_06645"
     CDS             complement(483..>1501)
                     /locus_tag="PLT08_06645"
                     /inference="COORDINATES: protein motif:HMM:NF024110.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNE04176.1"
                     /translation="ISQEIVAELEKSTAAYPELMSLADAEEEFSARRVSAVLVIPAGL
                     EISAIQNGTAELDFRQQPNDMDASIAQRAIQTAIRRVSSAVNAANVAVEEAKARGTFE
                     SAEAEQAYFEEALKVAKDLQANSPERITVIEGSTPDKVDYDPRANTSAGQLVTWVFIP
                     LFGISALFAYERQQGTLRRLLTTPTSKAVFLLGTIFGQVVMALIQMSLLILFGMFALQ
                     LKWGQEPLALFILLTCAALAAGAIGTTMGTFIKSEGQASGLSIMAGMVMALLGGCWYP
                     LELFPAAVQNIVKILPTTWAMQGLLDLVLRGGGLTDILLESGVLLGFAVVFFSIGVAR
                     FRYE"
     assembly_gap    1502..1601
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1602..2677)
                     /locus_tag="PLT08_06650"
     CDS             complement(<1602..2677)
                     /locus_tag="PLT08_06650"
                     /inference="COORDINATES: protein motif:HMM:NF024091.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNE04177.1"
                     /translation="MLKTFIIGIKDVKLAFRDRAALIFMLLAPFLLTLGMGLVTGRFS
                     GDNSSGISDIPVVIVNQDDGQLSKALVDLFYSEDLANLVEPSLGTSPEAARQMVDDDE
                     VATAIIIPAGFTDSIIPPTEMGMTIYVGPKLIEMYANPSRPTSAGIIKSIVDEFMSRV
                     DESTVSGQTAIQQLLTSGRVPPEQAQVAGTELNQRLTNSQQDEPLAIKLNATTADGEE
                     IKFDVLAYLAPGMALMFLMFTVSYGGRSILAEKSQGTLPRLLVSPTNSTQILGGKVFG
                     IFLTGVAQMLILIGGTSLMFQLKWGDTLGVLVLVFAAVFGATGWGMLITALAHTPAQV
                     GSVGSATMLIFGILGGSFIQLDNMP"
     gene            complement(2682..3626)
                     /locus_tag="PLT08_06655"
     CDS             complement(2682..3626)
                     /locus_tag="PLT08_06655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNE04178.1"
                     /translation="MASILEVQNLEKNYGDFKAVKGISFDIKEGEIFSLLGPNGAGKT
                     TTISMLSTLYTPTAGDATIAGHSITKAPMAVRNAIGVVPQDLALYEDLNARENLTFWG
                     QMYGLGGSALKNRVEEVLNQIGLVDKAKDRVKTYSGGMKRRVNIGVGLLHKPKLLFMD
                     EPTVGIDPQSRRAILDTVKDLNKQGMTVLYTTHYMEEAEELSNRVGIIDHGELIAIGT
                     QKELTQQVGETETLILHISENEDPEALVTAFKGIKGVLEAKSVDHEISVITPSAADVM
                     APVVTKANERGIKIHSIDIREPNLEAVFLHLTGRALRD"
     gene            complement(3643..4197)
                     /locus_tag="PLT08_06660"
     CDS             complement(3643..4197)
                     /locus_tag="PLT08_06660"
                     /inference="COORDINATES: protein motif:HMM:NF021886.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HNE04179.1"
                     /translation="MLKYALLGFLSYSPLTGYELKQRMDSSTNNFWNANLSQIYTSLK
                     SLEEDGWVKSKIQAQDDKPDKRIYTLTDEGLSSLKAWLEEPITDPEQRKSTFLLKLFF
                     SAKVDKETSLAQLRVSKNLHQREKERYKSEAQTSIKDIVATYPSMKKEAIFWEATRRF
                     GELYEEMYVHWLSETIELIENKMK"
BASE COUNT         1007 a         1013 c         1043 g         1077 t
ORIGIN      
        1 aatccagccg catggcgcgt ttgggtggcg agtttgccat gttgatgttg gtcacagtat
       61 cagacgcaga atttcagaat ctcgataaag attttcaaga aatgcgcggc gaaggctatc
      121 aagtcacctt gatgcagacc gaagatgttt ctaagaagta tgcaggttgg ctgccctatg
      181 agatcgaagt gaccggtgcc gaccatgaag gcatcattca acaaatttca catcatctgg
      241 cttcgcaggg tatcaacatt gaaagcatgg ataccagtgc aacgcctgcg ccgatgagcg
      301 gaactccgct ctttatgatg aaagggattg tgcttgtccc gccgaagttg aacttcaatg
      361 cctggagcga tgcattggaa gaagtggggg acaccttgaa tgtcagtgtg aagatcgaag
      421 ttgtgaaata aaagaaaagc aaacggcgga ttaacatcaa tccgccgttt ttattttaag
      481 tgttactcgt atcgaaatct tgcgacccca atagagaaga acacaaccgc aaacccgagc
      541 aaaacgcctg attcaagtag gatatcggtg agtccgccgc cgcgcagaac gaggtcaagt
      601 aaaccttgca ttgcccaagt agtagggaga atcttgacga tgttttgtac cgccgcaggg
      661 aataactcca acgggtacca acaaccaccc agcaatgcca tcaccatgcc tgccataata
      721 ctcaacccgc tggcttgacc ttcgcttttg atgaacgttc ccatggtggt gccaatcgct
      781 ccggcggcga gcgcggcaca ggtgagcaag ataaagagcg caagcggctc ttgcccccat
      841 ttgagctgga gagcgaacat cccaaacaga atcaacagcg acatttggat gagtgccatc
      901 actacttgac caaagatggt gccaagcaga aaaacggctt tactggttgg ggtagtgagc
      961 aaacgacgta atgtcccttg ttggcgttcg taagcaaaga gcgccgagat accaaacaaa
     1021 ggaatgaata cccaggtcac taattgccct gccgatgtat tggcacgcgg atcatagtcc
     1081 actttatctg gtgtgcttcc ttcaatgacg gtgatacgtt caggggaatt ggcttgaagg
     1141 tctttggcaa ctttcaatgc ctcttcaaaa taggcttgct ctgcctccgc ggactcaaac
     1201 gtgccgcgcg ctttcgcctc ttccacagct acgtttgcgg cgttgacagc actgctcaca
     1261 cgtcgaatcg ccgtttgaat ggcacgttgc gcaatggaag catccatgtc gttgggttgc
     1321 tggcgaaagt caagttcggc agttccgttt tgaatggcag aaatttccaa gccagcaggg
     1381 atcaccaaca ccgccgaaac acggcgcgca ctgaattctt cttcggcatc tgccagagac
     1441 attaactcgg ggtatgccgc agtggacttt tccaactctg ccacgatctc ttgcgagatg
     1501 gnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1561 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nggcatgtta tccaattgga
     1621 tgaaacttcc gccgaggatg ccgaagatca acatcgtggc agagccaaca ctgcctactt
     1681 gtgcaggcgt atgagccagc gcagtgataa gcatgcccca gccggtagca ccgaagacag
     1741 ccgcaaatac caacacaagt acaccaaggg tatcacccca tttcaattga aacatgagcg
     1801 atgtgccgcc gattagaatc aacatttgtg ctacgccagt gaggaagatg ccaaagactt
     1861 tgcctccaag aatttgcgtt gagttggtgg gggagaccaa aagccgaggc aacgtgcctt
     1921 gtgatttttc tgcaaggatg gaacgcccgc cgtagctgac tgtgaacatc agaaacatca
     1981 atgccatgcc aggagcaaga taggcaagca catcgaactt aatttcttca ccatctgcag
     2041 tggtcgcatt gagtttgatc gcgagcggct cgtcttgctg gctgttcgtg aggcgttgat
     2101 tcaactctgt gccagccact tgtgcttgct ctggcggaac gcgcccgctg gtgagcaatt
     2161 gctgaatcgc agtctgcccg ctaacggtgc tttcatccac gcggctcatg aactcgtcca
     2221 cgatcgattt gatgatgcct gcgctggttg ggcgtgatgg attggcatac atctcgatca
     2281 gctttggtcc gacgtaaatg gtcatgccca tctcagtggg cgggatgatg ctatctgtaa
     2341 acccggcagg gatgatgatg gcagtagcaa cttcgtcatc gtccaccatc tgacgtgccg
     2401 cctcaggact cgtgccgagg cttggctcaa ccaagttagc caagtcttcg ctgtagaaca
     2461 ggtccaccaa cgccttgctc agctgtccat catcctgatt gacaatcacc actggaatat
     2521 ctgaaatgcc agatgaattg tctccgctaa aacgcccggt gaccagcccc atgcccaagg
     2581 tgagtaggaa gggcgctaac agcatgaaga ttaatgccgc acggtcgcga aaggcgagtt
     2641 ttacatcttt gatgccgata ataaatgttt tgagcatgat gttaatcgcg cagtgcgcgc
     2701 cctgtgagat gtaagaagac ggcttccaaa ttcggttcgc gaatatcaat cgagtggatc
     2761 ttgattccgc gttcattggc tttggtcaca accggagcca taacgtctgc tgcagagggt
     2821 gtgatgaccg aaatttcatg gtcaaccgat ttggcttcga gcacgccctt aatgcctttg
     2881 aatgccgtca ctaaagcttc ggggtcttca ttctcgctaa tgtgcagaat caacgtttcg
     2941 gtttcgccaa cttgttgggt gagttctttt tgagtaccaa tggcgatcaa ctcaccatgg
     3001 tcaataatgc ccacgcggtt ggagagttct tcggcttctt ccatgtaatg agtggtgtag
     3061 aggacggtca tgccttgttt gttcaagtct tttaccgtgt cgagaatggc acggcgagat
     3121 tgtgggtcga tacccaccgt tggctcgtcc atgaagagca gctttggttt gtggagcaag
     3181 cccaccccaa tgttgacgcg gcgcttcatc ccgcccgaat acgttttgac acgatcctta
     3241 gctttatcca ccaacccaat ctgattcaac acttcttcca cgcggttctt aagagccgag
     3301 ccgcctagcc catacatttg cccccaaaaa gtgaggtttt cgcgagcatt gaggtcttcg
     3361 tatagggcaa ggtcttgcgg tacgacacca atggcattcc gcaccgccat gggggctttg
     3421 gtaatggagt gacccgcaat ggtcgcatcg ccagcggtgg gggtgtagag tgtggaaagc
     3481 attgagatgg tggtggtctt cccggcgccg ttcggtccga gtaagctgaa gatttcgcct
     3541 tctttgatat caaacgaaat cccttttaca gctttaaagt caccgtagtt tttttcgagg
     3601 ttttgtactt ccagtataga tgccatgatt gttcctttgt gttcacttca ttttgttttc
     3661 aataagttcg attgtttcag ataaccaatg tacgtacatc tcttcgtaaa gttctccaaa
     3721 tcttcgcgtg gcttcccaga aaatggcttc ttttttcata cttgggtagg ttgcgacgat
     3781 gtctttaata cttgtttgtg cttcagattt gtatcgttct ttttcgcgct ggtgcaggtt
     3841 tttggatacc ctaagctggg caagggatgt ttctttatcc acctttgccg agaagaaaag
     3901 ttttagcaag aatgtgcttt ttctctgctc agggtcggta ataggctcct cgagccacgc
     3961 ctttagactg ctaagccctt catcggtcaa cgtgtagatg cgcttatccg gtttatcgtc
     4021 ttgagcctga atctttgatt taacccagcc atcctcctct agagacttca acgaagtgta
     4081 gatttgacta aggttcgcat tccagaaatt gtttgtagaa ctatccatac gctgttttag
     4141 ctcgtacccc gttaaagggg aatagctgag aaacccgagt aaggcatatt tgagcataag
     4201 gcatccttta tatataaatt tttatatata actgtttata
//
LOCUS       DAOQJE010000198         4215 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_12753_length_4215_cov_5.864183, whole genome shotgun sequence.
ACCESSION   DAOQJE010000198 DAOQJE010000000
VERSION     DAOQJE010000198.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 4215)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4215)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4215
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12753_length_4215_cov_5.864183"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(337..3339)
                     /locus_tag="PLT08_06665"
     CDS             complement(337..3339)
                     /locus_tag="PLT08_06665"
                     /inference="COORDINATES: protein motif:HMM:NF037660.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HNE04180.1"
                     /translation="MSGYEKAIVKDGFLQASPNPIPIGSNLWFEWLSNAKKFSYKYPN
                     GAFVAQHEIRRNTSYWYAYKRRNGKLYKAYLGKTTELTLERLQQIGNFLRGDGVGDPP
                     PLSALTTDSHFFESRIDSSFLPITKITVPVLPRQLVSRGRLNQKISTPLTLIHAPSGF
                     GKSTLLNDWKQTCGYPIAWLTLDEHDNNPARFLYSVVTALQTIHSDFGTDLLTYISAA
                     TSINPAEAVNHLTKGILELQDFHPRLGIVLDDFHRINHSIIYDTLQVWLEHFPSNLQL
                     VISGHTRPPLSLGHLRAQGMATELDVNDLRFTNTEGVKYLQQYVQEPSLAYDDLEKLV
                     KHTEGWAAGLTLTALALSKQNDQRQFIDTFSGAHIYMREYFMETVLQRSNSEIQSFLL
                     RTAVLKNLTGSLCNAVTERTNSDEILEHLWQENLFIVRLEEQGWYRYHDLFAEMLCSQ
                     LQARYPNEVMHLHQRAAQWYREQYAPADAIFHLLSIEAWEEAATLIEEMALRELEQFG
                     EDSRLLRWLQDLPENVVQKHKNLLFVYMRLANNALSQKNIEIFINHIERIISNKPEIL
                     RTPDEKDVLAEIQEIKYTWAQGNTFVPPARSENDYDARWDLLNGLFLLKPAYSQCQEY
                     PEEPIINLLNAAQAQNNLFVTLMTVGSLTKRFVFAGQLRRAEKMCKQALEKALAQRGR
                     LPETASIALNVLGHVSFERNEMDLAQKYLSQSLDTDPNPTSSNMPIQNAILRSKIQVK
                     LGQFDEAIATIQTARNLHVRRPAGVWSSEDLLSYEIYIHLRNNNMDKAEELLHEGPTG
                     TGHHFMQQMQAELLLKNGHFEEAEEILWNVIDTFPTLIMIEPLQEVRILLSLALFWQN
                     KVNQSVQVMTGALRLSAPEKFIRPFLDWGKECAPILLLVSETQSLNGEAQEFLSKLLK
                     TLAITNEKTNPLKEDMEKLSTSASISIREQEILLLLSEGYSNREISKKLSVSESTVKT
                     HLGNIYMKLKVNSRMLAVSRAKQLKLIP"
     gene            3688..>4215
                     /locus_tag="PLT08_06670"
     CDS             3688..>4215
                     /locus_tag="PLT08_06670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HNE04181.1"
                     /translation="MKKILYVVSLLVIASMILTACGTPATEAPAATEAATEAAATEAP
                     AATEAASSEPADPNALPRNETLYFNGQQWGPVVGWNPYSSSMNNGLAIAQQDNARVTM
                     FETPYLYNMMDGKVYPLLADGDYQWNDAKTEITFKIKAAAHWSDGTPVTAEDVAYTWA
                     THIKYSTGIGAGNKDY"
BASE COUNT         1140 a          882 c          928 g         1265 t
ORIGIN      
        1 ttgatacgag gcagtggcag ctccccaaac aaagttatga ggaaaagtgt aattgttcat
       61 atattcaaat ccttttgtga aaatacttat ggacacactg ccgattaaac tcacgcttct
      121 ttttttgacc tttagcctgc tctacagact tagccctaat ctactctgtt gccgttctgt
      181 cattaatcca aatcgccctg ttgattacag ggcgaaagaa atatataaaa ggggagagat
      241 aaataagata gctttgaagg tctgcatgaa ttcagtactt tctgaaatcg attgcagagt
      301 ataaagagat tcagatagga tgtcaaaccc ccatgtttag gggataagct taagttgttt
      361 cgctcgtgat accgccaaca tacggctatt gactttaagt ttcatgtaaa tgttcccaag
      421 gtgagtcttg accgtacttt cagagacaga tagctttttc gatatttctc ggtttgaata
      481 cccttcactc aataaaagca atatttcttg ttcgcgaatg gatatggatg ctgaagtaga
      541 aagcttttcc atatcctctt tgagagggtt agttttttca tttgtaattg ccaatgtctt
      601 gagcaattta ctaagaaatt cttgtgcctc gccatttagg ctttgggtct ctgaaactaa
      661 taataaaata ggcgcgcatt ctttgcccca atctaagaag ggacgaataa atttctcagg
      721 tgccgagagg cgtaaagctc cggtcattac ttgaactgat tgatttactt tgttttgcca
      781 gaacaatgcc aaagataaca agatgcgcac ctcctgcaga ggctcgatca taatcaaagt
      841 tgggaatgta tcgatcacat tccatagaat ttcttctgct tcttcgaaat gcccattttt
      901 taacaagagt tcagcttgca tttgttgcat aaaatggtgc cctgttccag tgggtccttc
      961 gtgaagaagt tcttcggctt tatccatgtt gttgttacgg agatgaatgt aaatttcgta
     1021 agagagtaaa tcttcactgc tccacacacc agctgggcgg cgaacatgta agttgcgggc
     1081 ggtttgtatg gtggcaattg cctcgtcaaa ttgccctaat ttgacctgta tcttagaacg
     1141 cagaatggcg ttttgaatag gcatattact gctggtgggg ttgggatcgg tatctaaaga
     1201 ctgcgataga tatttctgtg ccaagtccat ttcattgcgt tcaaaactga catgtccaag
     1261 gacgtttaat gcaatactgg cggtttctgg caatctgcct cgttgggcga gcgccttttc
     1321 caaagcctgt ttgcacattt tttcggcacg tctcaattgc ccggcaaaca caaatcgttt
     1381 ggtgagtgaa ccaaccgtca ttaaagtgac aaaaagatta ttttgtgctt gtgcggcatt
     1441 taataaattt atgatcggtt cttcaggata ttcctgacac tggctatatg ctggtttgag
     1501 aaggaacaaa ccattgagta ggtcccatct ggcatcgtag tcattttcag acctggcggg
     1561 gggcacaaag gtatttccct gtgcccatgt gtacttaatt tcttgtatct ctgccaacac
     1621 atctttttca tcaggggtgc gaagaatttc aggcttattg gaaataatac gttctatgtg
     1681 gttgataaat atctcaatat tcttttgcga taaagcatta ttggctaacc tcatataaac
     1741 aaataataag tttttgtgtt tctgaaccac attttctggc aagtcttgta accaacgtaa
     1801 tagacgcgag tcttctccga attgttctaa ctcacgtaat gccatttctt cgatcaatgt
     1861 ggcggcttct tcccatgctt ctatggaaag taggtggaag atagcatctg ctggggcata
     1921 ttgttcacga taccattgcg ccgctctctg atggagatgc ataacctcat tcgggtatcg
     1981 ggcttgcaac tggctacata gcatttctgc aaaaagatca tggtatcgat accaaccttg
     2041 ttcttccaga cggacgataa atagattctc ctgccataaa tgttcaagaa tttcatcgga
     2101 gttcgtccgt tcagtcactg cattgcataa actacctgtc agatttttga ggacggctgt
     2161 tcttaacaaa aaggattgga tttctgagtt tgaacgctgg agaacggtct ccatgaaata
     2221 ttcacgcata taaatgtgcg ccccgctaaa cgtatcaata aattgacgct ggtcattttg
     2281 tttgcttaat gccaatgctg ttaaggttaa accagcagcc cagccttctg tatgtttaac
     2341 tagtttttca agatcatcat aggctagaga aggttcttgc acatattgtt gcaagtattt
     2401 aaccccttcg gtgtttgtaa aacgtaggtc gttgacatcc aattcagttg ccatgccttg
     2461 ggcacgtaaa tgacctaacg ataatggcgg acgggtatgc cctgaaatca ctagttgcag
     2521 attcgatgga aagtgttcaa gccagacttg aagcgtgtca taaataatag agtgattgat
     2581 gcgatgaaag tcatccaaaa caatgccaag gcgtgggtga aagtcttgaa gctcgagaat
     2641 cccttttgtt aggtggttca cagcttcagc agggttgatg gaagttgccg cactaatata
     2701 tgtaagcaga tctgtgccga aatctgaatg aatggtttgg agagccgtaa ccactgagta
     2761 taaaaaacgg gcagggttgt tatcatgttc atctaatgtt agccatgcaa tagggtaacc
     2821 acaggtttgc ttccagtcat tgagaagtgt gctttttcca aacccgctgg gcgcatggat
     2881 taaggttagt ggcgtactga ttttctggtt aaggcgtccg cgtgagacca actgcctagg
     2941 cagaacagga acggtgattt ttgtgatcgg aagaaatgac gaatcaatgc gtgactcaaa
     3001 aaagtgggaa tcagtagtga gggcactcag tgggggggga tcaccaaccc catcgcccct
     3061 taagaagttt ccaatttgct gcaatctttc aagggtaagt tctgtcgttt ttcctagata
     3121 tgccttatac agtttgccat ttcgccgttt gtaggcgtac cagtaggacg tatttcggcg
     3181 gatttcatgc tgtgctacaa atgctccatt tgggtactta taggagaatt ttttcgcgtt
     3241 ggaaagccac tcaaaccaca aatttgaccc aattggaatg gggtttgggc ttgcttgtag
     3301 gaaaccatct ttcacaattg ccttttcgta gccagacata ctatctccag tgtaaaaagt
     3361 tacccaacgc ttctaattat acctgttggg taactttttg cttaataatc ttttgttaac
     3421 gtagttttga ccacgaattt atagattttc tctcgtggaa aatcatccga aaacccgatt
     3481 aatttgttta tgcacctgaa ctattcttat aacgttagtg aagtatgttt taaagtgctt
     3541 tttcagagca cgcaaatata tattgctgat ataacaatta aataatataa cgactgtaaa
     3601 ggaggtggaa gccgcctgta attaattcat gaatgataga gtttcgatcg gttatttcaa
     3661 tccctaattt gaggagaaaa actcaccatg aagaaaattt tgtacgttgt ttctttgttg
     3721 gtcatcgcat cgatgatcct caccgcttgc ggtacccccg caacagaagc accggctgcc
     3781 accgaagccg caacagaagc cgccgcaact gaggctcctg ccgcgactga agctgcttcc
     3841 agtgaacctg ctgatcccaa tgctcttccc cgcaatgaga ccctgtattt caacggtcaa
     3901 cagtggggtc cagttgttgg ctggaatcct tactccagca gcatgaacaa tggtcttgcc
     3961 attgcccaac aggacaatgc ccgcgtgacc atgttcgaaa ccccctatct ctacaacatg
     4021 atggatggca aggtctatcc acttctcgct gatggtgact atcagtggaa tgatgccaag
     4081 accgagatca ctttcaagat caaggcagcc gctcactgga gcgatggcac ccctgttacc
     4141 gctgaagatg ttgcttatac ttgggcaacc cacatcaaat acagcactgg catcggcgct
     4201 ggcaacaaag actac
//
LOCUS       DAOQJE010000199         4213 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_12760_length_4213_cov_5.735690, whole genome shotgun sequence.
ACCESSION   DAOQJE010000199 DAOQJE010000000
VERSION     DAOQJE010000199.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 4213)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4213)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4213
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12760_length_4213_cov_5.735690"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..721)
                     /locus_tag="PLT08_06675"
     CDS             complement(<1..721)
                     /locus_tag="PLT08_06675"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNE04182.1"
                     /translation="MRSKSSDSASPALVLAAIGGLLFLHAPIWVIFLYSFTTDEATYT
                     FPLPGLTTKWFGAALQRVDLWNALTLSLQVAVVATLVALVLGTLAAAAVYRSDFFGKE
                     TISLLLILPIALPGIVTGIALRSAIGTLNIPFSYWTIVIGHATFCVVTVYNNVLARFR
                     RMQGSQVEASMDLGANSFQTFRYVVLPNIATALVAGGMLAFALSFDEVIVTTFTAGQQ
                     ATLPIWIFSQLVRPRDRPVTNV"
     gene            complement(711..1616)
                     /locus_tag="PLT08_06680"
     CDS             complement(711..1616)
                     /locus_tag="PLT08_06680"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNE04183.1"
                     /translation="MLNRISTYLYRHPRLVLGLLLGPPMLYMGVVYLGSLFSLLINSF
                     FYLEEFTGLVVREFTLQTYAKLLDPTNLGIFFRTATMALLVTIADAIIAFPLAYYIAR
                     FAPKKLKTYLYIAVTIPLWSSYLVRVYAWKLILAKEGIVSWFFNLFGLQFILDGILSI
                     PGIGGSSLSLSPIGMFIAFCYIWLPYMIVPIQTALERVPKSLLEASSDLGANPSQTFR
                     QVLLPLSFPGVVAGSIFTFSLTLGDFIIPQVLGNSRFFIGQAVYSYQGTAGNIPLAAA
                     FTMGPIVIMVVYLLIARKLGAFDAL"
     gene            complement(1609..>2582)
                     /locus_tag="PLT08_06685"
     CDS             complement(1609..>2582)
                     /locus_tag="PLT08_06685"
                     /inference="COORDINATES: protein motif:HMM:TIGR01187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNE04184.1"
                     /translation="QVEMEIRDGEFFSMLGPSGSGKTTCLRMIAGFDRPTSGHIYLYG
                     QDVSNLPPYERDVNTVFQDYALFPHMSVGDNIAYGLMIKKVEKVERMKRVEEMLDLVQ
                     LSGFAGRKPSQLSGGQRQRVALARALINHPKVLLLDEPLGALDLKLRQQMQVELKSIQ
                     KRVGITFIFVTHDQEEALTMSDRIAVFSQGKIEQIGTPSEVYEHPASQFVAGFVGTSN
                     LISGDVAERITGHAQTFSIRPEKIHIGSQQEYISSDMYAVDGTIRDVIYLGLYTRYLV
                     EITQGIDLVVISQNLKTTSADVFSSKGSAVRLSWQQDHISILGGKGA"
     assembly_gap    2583..2682
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(2889..4070)
                     /locus_tag="PLT08_06690"
     CDS             complement(2889..4070)
                     /locus_tag="PLT08_06690"
                     /inference="COORDINATES: protein motif:HMM:NF024808.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HNE04185.1"
                     /translation="MKKQLFNMLVVFALASLVLAACGGSGGTASEMKTEVGDGEGEVS
                     IISWAGYIERGETDPNYDWVTEFEAQTGCKVTNKTAATSDEMVTLMNEGGFDLVTASG
                     DASNRLIAGGKVQPINIDLIPSWSTVDDRLKDAPWHTVGGVHYGVPYQWGPNVLMYNT
                     EVFTEAPTSWDVTFEKMDLPDGKSNEGRVQAYDGPIYIADAALYLKYHKPELGIENPY
                     DLTEEQFNAVVELLTAQRSLINRYWHDAYVQMDDFKNEGVVASGSWPFQVNLLAFEGA
                     PIASTFPVEGATGWADTTMMHVDAPHPNCAYKWLEWSLNPKLQGDLAAWFGSVPSVPA
                     ACTGNELLTDAGCSANGFDQFDNIWFWRTPTAECSTGAGNCVPYSKWVEAYVGIISSG
                     Q"
BASE COUNT         1212 a          968 c         1000 g          933 t
ORIGIN      
        1 caacatttgt cacagggcgg tcacgcgggc ggacgagttg gctgaaaatc caaataggca
       61 aagtagcctg ttgcccagca gtaaatgttg taacaatgac ttcatcgaag gaaagtgcaa
      121 acgcaagcat gccgcctgct accaacgctg tggcgatatt cggcaagacg acatagcgaa
      181 atgtctggaa agaatttgcg cctaagtcca tcgaggcttc gacttgcgaa ccttgcatcc
      241 gacggaaacg cgctaacaca ttgttgtaaa ccgtcacaac acaaaaggtg gcatgcccaa
      301 tcacgatggt ccaataagaa aatgggatgt ttagtgtgcc aatagcggaa cgtaaagcaa
      361 tacccgtcac aatgccgggc aaggcaattg gcaaaattag caacagcgag atggtttctt
      421 tgccgaagaa gtcactacgg tacacagccg cagcggcaag ggtaccaagc actaatgcaa
      481 caagagtagc cacaaccgct acttgcaaag aaagcgtgag cgcattccac aaatccacgc
      541 gctggagcgc tgcgccaaac catttggttg tgagtccggg aagtggaaat gtgtatgtgg
      601 cttcgtctgt tgtgaatgag tacaggaaga tcacccaaat tggggcatgt agaaagagca
      661 agccgccaat ggctgcaagc accaaggcgg gagaagcaga gtctgaagac ttagagcgca
      721 tcgaaagctc ccaatttacg ggcgatcaat aaataaacaa ccataatcac gatgggaccc
      781 atcgtgaacg cagcagcgag tggaatgttg ccagccgtac cttgatagga atacacagcc
      841 tgaccaataa aaaagcgcga attgcctagc acttgaggaa taataaaatc acctaaagtt
      901 agggaaaatg taaaaataga acccgcgact acgccaggga aggacaaggg taacaatact
      961 tggcgaaaag tttgtgaagg gttcgcgccc aaatcactgg atgcttcgag caaagatttt
     1021 ggcacgcgtt ccaatgctgt ttgaatgggg acaatcatat atggcagcca gatatagcaa
     1081 aatgcaatga acatcccaat tggagaaagc gaaagagatg agccaccaat acctggaatg
     1141 gaaagaatgc catccaaaat aaattgcaac ccaaacaaat taaagaacca agaaacaatg
     1201 ccttctttgg cgaggatcaa tttccatgcg tagacacgta caagatagct ggaccaaagc
     1261 ggaatagtga cagcaatata caaataggtc tttaattttt tcggcgcaaa ccgcgcaata
     1321 taatatgcaa gcggaaaagc aataatggca tctgcaatgg ttactagcag cgccatggtt
     1381 gccgtacgaa agaaaatacc taggttggtt gggtctaaaa gtttggcgta tgtttgtaat
     1441 gtaaattctc gaacaaccaa acctgtaaat tcttcaaggt agaagaagct gttgatcaat
     1501 aacgaaaaca gagaccccaa atacaccaca cccatataaa gcatgggagg tccgagcaaa
     1561 agccctagca cgaggcgggg atgccggtac aaataggtgg agatgcggtt aagcaccttt
     1621 gcctcccaaa atactaatgt ggtcttgttg ccatgaaagc cgcacggcac tgcccttaga
     1681 cgagaagacg tcggcagagg ttgttttcaa attttgtgaa atgaccacaa ggtcaatgcc
     1741 ttgcgtgatt tcaaccaaat agcgagtgta caagccaaga taaatcacat cgcgaatggt
     1801 gccatccacg gcgtacatat ctgagctaat gtattcctgt tgtgaaccaa tatgaatctt
     1861 ttcagggcgg atggaaaaag tctgcgcgtg acctgtgatg cgttcggcga cgtctccgct
     1921 aatcaaattg gatgtaccca caaaccccgc cacaaactgc gacgcgggat gttcatacac
     1981 ttcagacggg gtaccaatct gttcaatttt cccctgacta aaaaccgcaa tgcgatcact
     2041 catggtgagc gcctcttctt gatcgtgcgt tacgaagatg aaggtaatac ccacccgctt
     2101 ttgaatggac ttaagctcca cctgcatttg ctggcgtaat ttcaagtcta atgcgccgag
     2161 gggttcatcg agtagcaaaa ctttggggtg attgatcaag gcacgggcaa gtgcgacgcg
     2221 ttggcgctgt ccgcctgaga gttgagaagg tttgcgtccg gcaaagccag agagttggac
     2281 gagatccagc atttcttcca ctcgtttcat acgctcaact ttttctacct ttttgatcat
     2341 gagaccatag gcaatattat cgcccacaga catatgaggg aaaagagcat aatcttgaaa
     2401 aacggtattt acatcccgtt catagggcgg aagattggaa acatcttgac cgtataaata
     2461 aatatgccca gaggtaggtc ggtcaaaccc cgcaatcatt cgcagacagg tggttttgcc
     2521 tgaacctgaa ggtcctaaca tggaaaaaaa ttcgccatcg cgaatttcca tttccacttg
     2581 atnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2641 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nngaaggtcc taacatggaa
     2701 aaaaattcgc catcgcgaat ttccatttcc acttgatcta cggcttttac atctccaaaa
     2761 tggcggctaa cagactcaaa acgaatcgca ggtgtatcca taaaactccg taaaaaagca
     2821 atattcaggc ggagccattg ccccgcctga atattttaca tcacgttact agaattctgg
     2881 cagcaggatt actgtccgct gctgatgatg cctacgtagg cttcaaccca tttgctgtaa
     2941 ggtacacaat tgccagcacc ggtggagcat tcggcggtcg gcgtgcgcca gaaccaaatg
     3001 ttatcgaact gatcgaaacc attggctgaa cagccagcat cggtgagcag ttcgttgccc
     3061 gtacaagcgg caggcacaga aggcacggaa ccaaaccaag ccgcaaggtc gccctgcagt
     3121 ttggggttca aggaccattc aagccacttg tatgcgcagt tggggtgagg agcatccaca
     3181 tgcatcatgg tggtgtctgc ccagccggtg gcgccttcaa cagggaaggt ggaggcaatc
     3241 ggtgcacctt caaaggcgag caggttcacc tggaaaggcc aggaaccaga agccacaacg
     3301 ccttcgttct tgaaatcgtc catttgaaca taggcatcgt gccaatagcg gttgatcaaa
     3361 gaacgctgag cggtgagcaa ttcaacgaca gcattaaatt gttcttcggt gagatcgtag
     3421 ggattttcaa taccgagttc tggcttgtga tacttgagat acaaagcagc gtcggcaatg
     3481 tagataggac cgtcataggc ttgaacgcgt ccttcgttgg acttgccatc aggaaggtcc
     3541 atcttttcga aggttacatc ccatgaggtt ggggcttcag tgaaaacttc tgtgttgtac
     3601 atcaacacat ttggacccca ctgataaggg acaccatagt gaacgccacc cacggtatgc
     3661 cagggagcat ccttgaggcg atcgtcaact gtagaccaag aggggatcaa atcaatgttg
     3721 atgggctgaa ccttgccacc tgcaatgagg cggttggagg catcgccaga agcggttacg
     3781 aggtcaaatc cgccttcgtt catcaaggta accatttcgt cagaggttgc agcagttttg
     3841 ttggttactt tacaacctgt ttgggcttcg aattcagtca cccagtcgta gttcgggtcg
     3901 gtttcgccgc gttcaatgta accagcccat gaaatgatgg aaacttcccc ttcaccatcg
     3961 ccaacttctg tcttcatttc agaagccgtt ccaccgctgc caccacaggc agcaagaacc
     4021 aggcttgcta aagcaaaaac aaccaacatg ttaaacagtt gcttcttcat atttctctct
     4081 cctattctct ttttgattag ttgaaacatc aggtcacttt atagaaatgg cttgatctct
     4141 tctgcaccga cctcctttta ggtcattagg aaagaattcc ccacatccaa tgacagggat
     4201 acggggatta ggc
//
LOCUS       DAOQJE010000200         4206 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_12796_length_4206_cov_7.037822, whole genome shotgun sequence.
ACCESSION   DAOQJE010000200 DAOQJE010000000
VERSION     DAOQJE010000200.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 4206)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4206)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4206
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12796_length_4206_cov_7.037822"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1293
                     /locus_tag="PLT08_06695"
     CDS             <1..1293
                     /locus_tag="PLT08_06695"
                     /inference="COORDINATES: protein motif:HMM:NF037660.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNE04186.1"
                     /translation="PPLYAHALSMQAYAFHTHDRNELAFQAASQAFDLSMATKDVSNA
                     VRALNIRAVSALMLGKTRGALDDLQQALALLEQHNLSASTQTVQSLYHLGTALVFSQD
                     YQQARAILSRAVELSKKGGLRRLEAAALTMLGQIALNCGQYKLAVEIYDRSIEVAGES
                     YLPGMWGKFAGRGWANLRMGNLLAARTDFERGLNVSIQVESQYGTILMRSYLAFTALA
                     AGESPSASFADLEADALTQALHPVVLFNANIQSQGWRLFGDSEKANSAHHRAIEAAKN
                     SDVPSFVQSARLQKLYTQVILEENIDPTYMEEELAELAKLTLDSGEVPLQVNTFLVRA
                     WLHSRMGNLQEALSTATQALTLARACPDLPWIGECQVLLAQLFTKLGDQNQAKICTEE
                     VHALAKTSFAPLTVKFGGEPLALVQEHLLKSLTPTHLN"
     gene            1396..2013
                     /locus_tag="PLT08_06700"
     CDS             1396..2013
                     /locus_tag="PLT08_06700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE04187.1"
                     /translation="MRNLLLAIFILALASMACNMPGSAGSPVNPPIEIPTFTATVPSV
                     VSDKPTDLPAPATSTPEQAVLQPQVNTFDPRSIADCDIFVDSDFPNTIGALPDTKKAL
                     PETDKKACQYNFANGTLFVSIFTSMPGMEAFENVRQFDALTGATIIPYPIGEAAVFKD
                     FGDGHITLEAVINGWYVVLDARGFDEKNLALLAELLLANLAPYSP"
     gene            2026..2259
                     /locus_tag="PLT08_06705"
     CDS             2026..2259
                     /locus_tag="PLT08_06705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE04188.1"
                     /translation="MNERRKAASFLIRVWQEPSQWQPPGEWRVSAHPLEGGGEVLFNS
                     ATELWRYLTHADSQIQSTPQTKKIISEGEENEQ"
     gene            2249..>4206
                     /locus_tag="PLT08_06710"
     CDS             2249..>4206
                     /locus_tag="PLT08_06710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE04189.1"
                     /translation="MNNKRSLGCVLTGALIILVICGAIAGFATWRLNTRGPEILVEIK
                     RPQTTMLANVNEALPLIVSAEASDIITRLEVYADGALIAAANGEGQNAAMLAQTWTPS
                     TVGRHVLVARAFLDAEHFSDSEVVFVDALDLSALPLQVNVDDLPRGEGVTEISVGDLA
                     TASGTTLEEIVRLNPSLSSTGSIPSGTLVSIPRLAPEPGIGSPVPPPAPGAPGSPAPS
                     TSDPHFEGETHSCNQIAMRWTGSDFETGYTLYRLAPGDSSMTLVATLPVTTVSYADNS
                     ISRAGTYRYFLSPIWRGGGSGITSMLAVEIPADCAPVSSTSANLRLSLMSLTAQESFT
                     GVYCYVSVNGSRYERLPAGDGLLRPTSGNLYYDLPLQLPNRGQYTLGPTADGLVRLEG
                     ECWGRRGVESVRIGRFAGNHASSEWDGRDLTSDVAFEPIEVASLDTIPLMTGGSNFIR
                     YRIQSAASHFDLTGLYNGAIQVPLAITDPFRIENPTVPPPTNVRLVNRASGICEDLPM
                     DDPNLGTILCQEQIVRHIAWDWSASEQVPQSAVTGFMLITSIEDAISTTPAGEGYTHN
                     VNSGTARSAPVPNYSQRWRCGATVRFTVRAVTNFGMSEPSEAFEVRLPACPSARNLLI
                     RIESLTISPSASTGQVLDRGDICILCDDR"
BASE COUNT         1019 a         1052 c         1029 g         1106 t
ORIGIN      
        1 ccgccactat atgcacatgc tctttcgatg caagcctatg cttttcacac ccacgaccgc
       61 aatgaacttg catttcaagc tgcttcgcaa gcatttgacc tttccatggc aacaaaagat
      121 gtttcgaatg cggtgcgtgc tttaaacatt cgcgcagtga gtgccttgat gctgggaaag
      181 acacgtggcg cgttggatga tcttcagcaa gcacttgcct tactggaaca acacaacctt
      241 tctgcatcaa cgcaaactgt acaaagtttg tatcatctcg gcactgctct tgtcttttca
      301 caggattatc aacaagcacg agccatactt tcccgtgcag tggaactttc aaaaaagggc
      361 ggactgcgtc gtctagaagc ggctgcgttg accatgttgg gtcagatcgc tttgaactgt
      421 ggccaataca aacttgctgt tgaaatttac gatcgatcca ttgaagttgc tggagagtcg
      481 tacttgcccg gtatgtgggg taaatttgcc gggcggggtt gggcaaattt acggatggga
      541 aatctactgg cagctcgcac agactttgaa cgcgggttga atgtttctat tcaagtagaa
      601 agccaatatg gaacaatatt gatgcgttct tacttggcat tcactgcact tgcagctgga
      661 gaatctcctt ccgcttcatt tgcggacctc gaagcggatg cgctgaccca agccttacat
      721 cccgttgtgc tttttaatgc caatatccaa tctcaggggt ggcgcttatt tggggatagc
      781 gaaaaagcaa attcggcaca ccaccgcgcg atcgaggctg ccaaaaatag tgacgttcct
      841 tcttttgttc aatcagcaag attgcaaaaa ctatatacgc aagtcatcct tgaggagaac
      901 attgacccaa cttacatgga agaggaactc gctgaattag ccaagctgac tcttgattct
      961 ggcgaagtgc cattacaagt caacacattt ttggtgcggg cgtggttgca ttctcgtatg
     1021 ggcaatttac aagaagcgct ttccactgcg acccaagcac taaccttggc gcgcgcctgc
     1081 ccagacctgc catggatagg agagtgccaa gtcttacttg cccagctttt cacaaaactt
     1141 ggggatcaaa atcaggcaaa gatatgtaca gaagaagtgc atgccttggc gaaaacttcc
     1201 tttgcacctt taaccgttaa atttggagga gagcctttag cgcttgtgca agagcattta
     1261 ttgaaaagtc ttacacccac acatttgaac tagtccttcc gtactaaact cttgaaggta
     1321 aggggaaaca cttgtttaga ggacgttttt aggacgctct ctctgtaaga tatggggcaa
     1381 aaaggagaga aaagcatgcg aaacctgcta ttggctattt ttattttggc tctggcttcg
     1441 atggcttgta atatgcctgg ctcagccggc tcgcctgtca acccgcccat tgagattcct
     1501 acttttacag ctacggttcc cagtgtagtg tcagataaac caactgactt gcccgcccct
     1561 gcaacatcca ctccagagca agctgtatta caaccgcaag tcaacacatt tgacccgcgc
     1621 agtatagctg actgcgatat ttttgttgac tccgattttc caaacaccat tggcgctctt
     1681 ccagatacga agaaagctct tcccgaaact gataaaaaag cctgccaata caattttgca
     1741 aatggcacac tctttgtttc catattcacc agcatgccgg ggatggaagc ctttgagaat
     1801 gtccgtcagt ttgacgctct gacaggtgca acaataatcc catacccaat cggtgaagcc
     1861 gccgtattta aagattttgg tgatggacat atcacactcg aagcagtcat caacggttgg
     1921 tatgtggtat tggatgcacg cggcttcgac gaaaaaaatc tggcactgct ggcggaatta
     1981 ttgcttgcca atctggctcc ctattcgcct taacggagct tatccatgaa tgaaagacga
     2041 aaagcagcat cttttctgat tcgggtttgg caggaaccga gtcagtggca gccccccggc
     2101 gagtggcgag taagcgctca cccgttggaa ggaggcggtg aggtcttgtt caactcggca
     2161 actgagctat ggcgctacct cacccatgca gactcgcaaa tccagagtac ccctcaaaca
     2221 aagaagatta tttcagaagg agaagagaat gaacaataaa cgcagtcttg gttgtgttct
     2281 aacgggagcg ttgataattc ttgtaatttg cggcgccata gcaggttttg ctacgtggcg
     2341 attgaataca cgcggacctg aaatcttggt tgagatcaaa agaccacaaa cgaccatgct
     2401 tgctaatgtc aatgaagcgt tgccgttgat cgtctctgct gaagcaagcg atattatcac
     2461 ccgtcttgaa gtgtatgcag atggtgcttt gattgctgcc gctaatggtg aggggcaaaa
     2521 tgctgccatg ttggcacaga cttggacgcc ctctacagtc gggagacatg tgcttgtagc
     2581 gcgagcattt ttagatgccg aacatttttc ggattcagaa gttgtttttg tggatgcgct
     2641 tgacttgagt gcattgccac ttcaagtgaa tgtagatgac ctgccgcgcg gtgaaggtgt
     2701 gactgaaatt tcagtgggtg acttagccac cgcctcggga accacgctgg aagaaattgt
     2761 tcggttgaac ccaagtcttt cctctactgg aagtattcct tcaggtacgc ttgtttctat
     2821 tccgcgcctt gctcccgaac ctggtattgg tagccctgtg ccgccgcctg ccccaggtgc
     2881 tccgggatcc cctgcacctt caaccagcga cccgcatttt gaaggtgaga cgcattcctg
     2941 taatcagatc gcgatgcgct ggactggcag tgactttgaa actggctata ccctctatcg
     3001 acttgcccca ggcgatagct ccatgacgct tgttgcgact ttgccagtaa caaccgtttc
     3061 ttatgcagat aactcgattt cccgagccgg aacatatcgc tatttccttt ctcccatttg
     3121 gcgtggcggt ggcagtggta tcacatccat gctggcagtg gaaattcctg ccgattgtgc
     3181 accagtttcc agcacatcag caaacttgcg tctttcactt atgagtctca ctgcgcagga
     3241 aagttttacc ggcgtgtatt gttatgtctc ggtcaatggg tcacgttatg aacgccttcc
     3301 agcaggggat ggcttgttgc gccccacctc tggtaattta tattacgacc ttcctctgca
     3361 attgcccaac cgcggacaat atacccttgg tcccactgcc gacggcttgg ttcgtttaga
     3421 gggagaatgt tggggcaggc gcggagtaga aagtgtgcgc attgggagat ttgcaggcaa
     3481 tcacgccagt agtgaatggg atggacgcga cttaactagt gatgtggcat ttgaacccat
     3541 tgaagttgct tcgctagaca ccatacctct tatgacgggt ggttccaact ttatccgcta
     3601 tcgcattcaa tctgcagcca gccattttga cttaacgggt ttatacaatg gcgcaattca
     3661 ggttccgctt gcgattacag atccatttcg aatagaaaac cctaccgttc cgccgcccac
     3721 gaacgtacgt ttggtcaacc gtgcatctgg catttgtgaa gacctgccaa tggacgaccc
     3781 caatctcggc acgatcttgt gtcaggagca gatcgtccgt cacattgcat gggattggag
     3841 cgctagcgaa caagtgccgc aatcagcagt gacgggtttc atgctcatca cttctatcga
     3901 ggatgcaatc tcaactaccc cggctggtga gggctacacc cataatgtca actcggggac
     3961 tgcgcgttct gcacccgttc caaactattc acaacgttgg cgttgcggcg cgaccgtgcg
     4021 attcaccgtc cgtgctgtta caaatttcgg catgtcagaa ccgagcgaag cctttgaggt
     4081 gcggctgccg gcttgcccat cagctcgcaa tcttttgatc cggatcgagt cactcaccat
     4141 cagccctagt gcatctacag ggcaggtatt agaccgcggc gatatctgta ttttgtgtga
     4201 tgaccg
//
LOCUS       DAOQJE010000201         4201 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_12826_length_4201_cov_4.746503, whole genome shotgun sequence.
ACCESSION   DAOQJE010000201 DAOQJE010000000
VERSION     DAOQJE010000201.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 4201)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4201)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4201
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12826_length_4201_cov_4.746503"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(44..1333)
                     /locus_tag="PLT08_06715"
     CDS             complement(44..1333)
                     /locus_tag="PLT08_06715"
                     /inference="COORDINATES: protein motif:HMM:NF017760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNE04190.1"
                     /translation="MHIPPSLKHKKFFYLWLGQLISITGTQMQLWALFWHINQIDKNP
                     IALGGIGVARILPVIIFSLIGGALADSVDRRKVMFATQSVAAVLALTLALFTQFGQVT
                     IWHIYIITALQAVVVAFDGPSRQALIPNLLPKEDLPNAFSMTFTAFQAGAVIGPALSG
                     FFIAGFGQEAVYYFNAISFVAVLVALLMIGDVPQAIGEKTAGISMESIKDGIRFILSK
                     PIIFSTMMVDFVATFFASASTLMPIIAKDVLDVGVVEYGYLSAASAVGAVAVALVISQ
                     VKEIRKQGLVFVVAVIIFGIATIIFGITRSFIVAWLAIAVTGGADGVSTIVRNTIRQL
                     QTPDHVRGRMTSINQIFFMGGPQLGEIEAGAVAQFFGAPIAVITGGIGCIVGTLWIVW
                     KWPQLKNYNGDEPIEAGGSFPSPSTGASIIANRVRKQ"
     gene            complement(1402..2229)
                     /locus_tag="PLT08_06720"
     CDS             complement(1402..2229)
                     /locus_tag="PLT08_06720"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008795 - NAD+ synthase activity [Evidence
                     IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD+ synthase"
                     /protein_id="HNE04191.1"
                     /translation="MIDINLSINTDLAREILSGFIKSEITRIGMSRAVIGLSGGIDSA
                     LSLALAVDALGKDNVLAVRMPYKASSKDSLDHAQLLIDQLGVPSKTIEITDMVEPLFK
                     LSPDMSKLRKGNIMARERMIVIYDQSEVFKGLVVGTSNKTEILLGYSTLFGDSANALN
                     PIGDLYKTQVRQLSRAMNIPSPIVDKPPSADLWEDQTDEGELGFTYSEADRLLYLLVD
                     QRYSPQEAIEAGFDEKFVNAVTTRIRRNQFKRMLPPIAKISNRTIGYDFLYLRDWGT"
     gene            complement(2222..3073)
                     /locus_tag="PLT08_06725"
     CDS             complement(2222..3073)
                     /locus_tag="PLT08_06725"
                     /inference="COORDINATES: protein motif:HMM:NF012993.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrilase-related carbon-nitrogen hydrolase"
                     /protein_id="HNE04192.1"
                     /translation="MKLKLALAQINTKLGDVEANLAKHLEYIERAKAEKADLLVFPEL
                     SLTGYVLQDLVASVAHKPTEDDPVFRHLLKASHDLDLVVGFVDEDSRHRFFIASAYLS
                     GGKVLHVHHKVYLPTYGLFDEGRFFAWGDSVRSFDTKFGRVGMLICEDFWHASPPYLL
                     WLDGADIMLFSSASPGRGLNDKDKLESARWVERITKAYASMFTSFVAHANRVGYEDGL
                     HFWGGATIFDPTGEELAHGPYNEEAITYSELDLNQLRRTRARLPLLRDERTALVQKEM
                     NRILARD"
     gene            complement(3109..>4201)
                     /locus_tag="PLT08_06730"
     CDS             complement(3109..>4201)
                     /locus_tag="PLT08_06730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE04193.1"
                     /translation="TELPEPVASVEECKLPPLSFTNVGLGLPNPAFKMPSVGDVKTIV
                     LFADFSDVPATQTPEELLSIISPGAEAFYSEMSYGRMRWTLEPHPVWLRLSQPSAHYG
                     EGIRSYDGHLAFIQEAVTLADADVDFSTADSVIVLVPPEASAIPYGPAFGASPGGGYF
                     ADGKTFANGATSGADLPGWGSYWLNHETGHMMGLPDLYAYQYDPNNYPDQHRFVGDFS
                     LMGFIDGSAPGFFAFERWQLSWLDDEQIICQTTSDQTTTLTPIEIQGGIKAVIVPISE
                     SKALVVESRRPVGADAELVKSGALVYLADTSIYSGEGTITVLPVSGDDPYRVQSPLAV
                     GETLTVEGITVTVTASDDQSDTVQVVVTK"
BASE COUNT         1162 a         1044 c         1004 g          991 t
ORIGIN      
        1 gaaataaaag agacagtcgc ctgcgaggtg actgtctctt ggtttactgt ttccgcacac
       61 gatttgcgat gatcgaagcc ccagtagatg ggcttgggaa tgacccacct gcttcgatgg
      121 gttcatcacc gttatagttt ttgagttggg gccacttcca cacgatccac aaggttccaa
      181 cgatacaacc aatgccgcca gtaatgacgg caatgggcgc gccaaagaac tgcgccacgg
      241 ctccagcttc gatctcgccg agctgcggtc cgcccatgaa gaagatctgg ttgatactcg
      301 tcatccgccc gcggacgtga tccggcgttt gcaactgacg gatggtattg cgtacgatgg
      361 tgctgactcc atcggcaccg ccggtgacgg caattgccag ccacgccaca atgaatgacc
      421 ttgtaatccc aaaaatgatc gttgcaattc caaagataat gacagcaacc acgaacacca
      481 gtccctgttt gcgaatctct ttaacctgtg aaataaccaa tgctactgcc actgctccca
      541 ccgccgaagc ggcggagagg taaccatatt ccactacacc tacatccaat acatctttgg
      601 caatgatggg catcaaggtg ctggcagagg caaagaaggt cgcaacaaaa tcaaccatca
      661 tggtggaaaa gataatcggt ttggagagga taaagcgaat gccgtctttg atagattcca
      721 tactgatgcc cgctgtcttt tcgccaatgg cttgagggac atctccgatc atcagaagag
      781 cgaccaaaac cgctacaaat gaaatcgcat tgaagtaata aactgcttcc tgaccaaagc
      841 cagcaatgaa gaagccactc aaggcgggtc caatcacagc gccagcttga aaggctgtga
      901 atgtcatact gaaggcattt ggcaaatcct ctttgggtaa caagttcgga atcaacgcct
      961 gacgagatgg accatcaaac gccacgacca ccgcttgcaa tgcagtaatg atgtaaatgt
     1021 gccagatggt gacctgccca aattgtgtga acaaagccaa ggtcagagca agaaccgcgg
     1081 caacagactg tgtggcaaac atcactttac gtctatccac tgagtctgct agtgcaccac
     1141 cgatcaaaga aaagatgatg acgggcaaaa tacgcgcgac accaatgcca ccaagcgcaa
     1201 tggggttttt gtcaatctgg ttgatatgcc aaaataaagc ccagagttgc atctgggttc
     1261 cggtgatgga gatgagttgt ccgagccaaa gataaaagaa ttttttatgt tttagggaag
     1321 gaggaatatg cattttatta tttgtagggg cgaggcgata ccactttgtg gcacaagcct
     1381 tgcccctact ccattatttt tttatgttcc ccaatcgcgg agatataaga agtcgtagcc
     1441 gatggtgcga ttgctgatct tggcgatcgg cggaagcata cgtttaaatt gattgcgtcg
     1501 aatgcgtgtt gtgacggcgt tgacaaattt ctcatcaaag ccagcttcaa tcgcctcttg
     1561 cggactgtag cgctgatcca ccaaaagata gaggagcctg tccgcttcag aataggtaaa
     1621 gcctaattcg ccttcgtcgg tttggtcttc ccatagatca gcggaaggtg gtttatcgac
     1681 aattggagat ggaatattca tcgcccttga aagttgacgg acttgcgtct tgtacaaatc
     1741 tccaatggga ttaagagcat tcgccgagtc accgaagaga gtgctgtacc ccaaaaggat
     1801 ttcagtctta ttgcttgtac cgacaactag ccctttgaac acttcgcttt ggtcgtagat
     1861 cacgatcatc cgttcacgag ccatgatatt gcctttgcgc agtttggaca tatcgggact
     1921 gagtttgaac aagggctcaa ccatgtcggt aatttcaata gttttgctgg gcacacccaa
     1981 ttggtcgatc aataattgag cgtgatccaa cgaatctttg ctagacgctt tatacggcat
     2041 gcgcaccgca aggacattat ccttcccgag cgcatcaaca gcaagcgcaa gagaaagcgc
     2101 cgagtcaata ccaccagaaa gcccgattac cgcgcgtgac attccaatgc gggtaatttc
     2161 agatttgatg aaacctgaga gaatctcgcg agccaggtct gtattgatgc taaggttaat
     2221 atcaatcacg ggcgagaatc ctgttcattt ctttttggac gagagccgtg cgttcatcgc
     2281 gcaacaaagg taagcgggca cgtgtgcggc gaagttgatt caaatccaat tcagagtatg
     2341 tgatggcttc ttcgttatag ggtccatgcg ccagttcttc tcccgttggg tcaaagatcg
     2401 tcgcaccacc ccagaagtgc agtccatctt cataacccac tcggttggca tgcgctacga
     2461 acgaggtgaa catactggca taggctttgg tgatgcgttc cacccagcgt gcggattcga
     2521 gtttgtcttt gtcgttcagt ccgcgacccg gcgaggcgga agagaaaagc ataatgtcgg
     2581 cgccatcaag ccagagcaag tatggaggcg aagcgtgcca aaaatcttcg cagataagca
     2641 tgcccactcg tccaaacttc gtatcaaacg agcggacaga gtctccccac gcaaagaaac
     2701 gaccttcgtc aaaaagtcca taggtcggta aatatacttt gtgatgcaca tgcaaaactt
     2761 ttccacccga aaggtatgca gaggcaatga aaaagcgatg acgtgaatct tcgtctacaa
     2821 agccaaccac caagtcaagg tcatgagacg ctttgaggag gtgcctgaag acagggtcgt
     2881 cctctgttgg tttgtgtgcc accgaagcga ccaaatcttg taagacataa ccggtaaggg
     2941 aaagttctgg aaaaacaagt aaatccgctt tttcggcttt tgcgcgttca atgtattcaa
     3001 ggtgtttagc gaggttagct tccacatcgc caagctttgt attgatctgt gcgagagcga
     3061 gtttaagttt cattgcgaga atcttaccac taaaaaatac aagcaaaatt attttgttac
     3121 tacgacctga acggtatcac tctggtcgtc tgaagcggtc acagtcacag taattccttc
     3181 taccgtaagt gtttcgccta cagccaaagg agattgcacc cgatacgggt catcccctga
     3241 aacaggcaat acagtaattg tcccttcgcc agaataaata gatgtatctg ccaaatatac
     3301 caatgcccct gatttgacaa gttccgcatc cgcaccaacg ggacggcggg attcaaccac
     3361 cagagcttta ctttcgctga tgggtacgat cactgcctta atgccacctt gtatttcgat
     3421 cggcgtgaga gtcgtcgttt ggtcagaggt cgtctggcaa ataatctgtt catcatccaa
     3481 ccaactcaat tgccagcgtt caaaagcaaa gaaacccggc gcactaccat caataaagcc
     3541 catcaaacta aaatcaccca caaagcgatg ctggtctgga taattattgg ggtcatattg
     3601 atatgcataa agatctggca accccatcat atgcccggtt tcatggttca accagtatga
     3661 tccccaaccc ggcaaatctg cgccagacgt ggcaccattg gcaaaagtct tgccatcagc
     3721 aaaatagccg ccgccaggcg atgcaccaaa ggcaggtcca tacgggatgg cgcttgcctc
     3781 aggtggcacc aacacgatca cagaatcagc tgtagagaaa tcgacatccg catccgcaag
     3841 agtaaccgct tcttgaatga acgccaaatg accgtcataa cttcgaatgc cttcaccata
     3901 atgagcagaa ggctgactca atcgcagcca aaccggatgc ggttcaaggg tccatctcat
     3961 tctgccatac gacatttcgg aataaaacgc ctctgcaccg ggtgaaatga tgcttagtaa
     4021 ttcttcaggg gtttgcgtgg caggcacgtc cgaaaaatcg gcaaagagga cgatcgtctt
     4081 tacatcaccg actgacggca ttttgaatgc tgggttcggc aatcccaagc caacattggt
     4141 aaaggaaagt ggcggcaact tacattcttc cacagaagct actggctctg gcaattctgt
     4201 a
//
LOCUS       DAOQJE010000202         4186 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_12908_length_4186_cov_7.469620, whole genome shotgun sequence.
ACCESSION   DAOQJE010000202 DAOQJE010000000
VERSION     DAOQJE010000202.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 4186)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4186)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4186
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12908_length_4186_cov_7.469620"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..256)
                     /locus_tag="PLT08_06735"
     CDS             complement(<1..256)
                     /locus_tag="PLT08_06735"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014612531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HNE04194.1"
                     /translation="MTNSSFQEIILKLQNFWASKGCLITQPYYTQVGAGTMNPATFMR
                     VLGPEPWNVAYVEPSVRPDDGRYGENPNRLQKHTQFQVILK"
     gene            379..735
                     /locus_tag="PLT08_06740"
     CDS             379..735
                     /locus_tag="PLT08_06740"
                     /inference="COORDINATES: protein motif:HMM:NF014021.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HNE04195.1"
                     /translation="MAQISAKPKTNKSTMPDKHRFVVLKTKVTPLLKPYASRISVFGS
                     YARGDMTASSDIDILIALKPAKSRPPLGLFGFIALEQNLAKKLGRPVDLVTEESINPR
                     RRKSIQQDRVVLYEAK"
     gene            722..928
                     /locus_tag="PLT08_06745"
     CDS             722..928
                     /locus_tag="PLT08_06745"
                     /inference="COORDINATES: protein motif:HMM:NF014043.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF86 domain-containing protein"
                     /protein_id="HNE04196.1"
                     /translation="MKQNRDLVYLGDILDAIRRIEDYTAGIKKKEFMEHLMMQDAVMR
                     QIEIIGEASNSVSDDFQEKHPELP"
     gene            complement(1353..2900)
                     /locus_tag="PLT08_06750"
     CDS             complement(1353..2900)
                     /locus_tag="PLT08_06750"
                     /inference="COORDINATES: protein motif:HMM:NF015722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HNE04197.1"
                     /translation="MPEPKRPLKVFLCHASTDKPKVRELYRYLKKRGIQPWFDEEDLV
                     GGQDWQVEIPKALATSDAIVICLTKNSVDKEGYIQKEIKFALDKALEMPEGRIFLIPV
                     KFEECEVPFTLSRYQWVDLTVESGYAKMMKALKFRASQLERSTVEISKKDVEEEYLVL
                     GKTDRKSNEPVEQKSKEKRSPEQQIYETIKKPRQKLTIRKLFGNTVLIFWSIFVFGAI
                     ALMWKPFFQMIAPATEEKTPQVATTSPYTETVAASTLESTIKPTLLPTEITDAKGVSM
                     VLVPAGEFSMGIDNSNNGNQDANLSHIVYLDDYYIDKYEVTNAQYKTCVEIGACTLPE
                     FTNSSTRSNYFDNPNYNDYPVVNVSWLQADTYCSWRKVRLPTEAEWEKAARGIDGRTY
                     PWGEDLNCINTNYSGCAGNDTVAVGSFLNDKSPYGAYDMGGNVEEWVWDWYSATYYQS
                     SPYANPLGPEDGENRVVRGGAWVWGSVSTFYNSLEPLKVFTRHWWYSTDQGYEHIGFR
                     CASDVVP"
     gene            2985..3413
                     /gene="tcmP"
                     /locus_tag="PLT08_06755"
     CDS             2985..3413
                     /gene="tcmP"
                     /locus_tag="PLT08_06755"
                     /inference="COORDINATES: protein motif:HMM:TIGR04474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="three-Cys-motif partner protein TcmP"
                     /protein_id="HNE04198.1"
                     /translation="MDKYLLPEDDGLPARTFGKWTTAKTDYLQRYVDLFETSMRGKPW
                     CARCYIDLYSGAGKYQVEGQTGFHLGSPLIGITTKYPFTHYFFADISQENIDILKQRC
                     EPASQIRKKFYVRDANLLAQEIVNEIKSIERNRSKEQWPL"
     gene            3401..3853
                     /gene="tcmP"
                     /locus_tag="PLT08_06760"
     CDS             3401..3853
                     /gene="tcmP"
                     /locus_tag="PLT08_06760"
                     /inference="COORDINATES: protein motif:HMM:TIGR04474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="three-Cys-motif partner protein TcmP"
                     /protein_id="HNE04199.1"
                     /translation="MASLNLAFLDPNGLELEWSTIESLASVNKMDLIIYYSQSGLTRN
                     FENCINVDGDTDIDRFFGDREWRDIFKTSHGKSAGLHRKLIDQYKHKLSILGYLDVKD
                     IEDGAEPLMKNSKDAPLYRLIFASKHARGHDFWNKIIKKDAFGQGKLF"
     gene            3921..>4186
                     /locus_tag="PLT08_06765"
     CDS             3921..>4186
                     /locus_tag="PLT08_06765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5131 family protein"
                     /protein_id="HNE04200.1"
                     /translation="MAEHSGIEWTESTWNPLTGCTKISPGCKHCYAERMAKRLQAMGQ
                     PNYRNGFKLTLHENAIEKPLEWKKPQVIFVNSMSDLFHKDVPVE"
BASE COUNT         1223 a          866 c          930 g         1167 t
ORIGIN      
        1 gcttgagaat cacctgaaac tgggtgtgct tttgcaaacg gttgggattc tcgccatagc
       61 gcccgtcatc ggggcggacg gacggctcaa cataagccac attccacggc tcagggccga
      121 gcacgcgcat gaaggtggcc gggttcatcg tccctgcgcc gacctgggtg tagtacggct
      181 gtgtgatgag acatcccttg gatgcccaga agttttggag tttgaggatg atttcttgga
      241 aggaggagtt cgtcatgtac gatttccgat cttgggatgc ttcgacaatc tcagcacaag
      301 ttttggattg atgattgcgg ggggattata actgatgggg aatggatggc aggttaagaa
      361 attcggtata atatttatat ggctcagata agcgcaaaac caaaaacaaa caaaagtacg
      421 atgcctgaca agcatcgttt tgtggttttg aaaacgaaag ttaccccatt gttgaaacca
      481 tacgccagcc gaatttctgt tttcgggtcg tatgcccgcg gagacatgac tgcttctagc
      541 gatattgaca ttttaatcgc actgaaacct gctaagagcc gcccaccatt ggggttgttt
      601 ggtttcattg ccctggaaca aaacctagca aaaaaactgg gacgccctgt ggacttggtg
      661 acagaagaaa gtattaatcc acgccgaaga aaaagtattc aacaagacag ggtagtactt
      721 tatgaagcaa aatagggact tggtctacct cggtgatatt ttggacgcta ttcgccgaat
      781 tgaagactac actgccggta taaagaaaaa ggaattcatg gaacatctga tgatgcagga
      841 cgccgtaatg cggcaaatag agatcattgg cgaagcgtcc aatagtgttt cagatgattt
      901 tcaagaaaag caccctgaac tgccgtagtt tcaaatgcgc gggatacgca ataagatcat
      961 ccatgactaa cggggaatta atttacaggt catttgggat acggtgaaga atgatctgcc
     1021 cgccctcaag aaacaggttg aagagatttt aggggagtag taggtaaaaa tgagaactcc
     1081 gagattttga aaatctcggg gttcttttat tcatcgtccc tgcaccgacc tgggtgtagt
     1141 agggctgcgt gatgagacat ccgtgcgatg cccagaagtc ttggagtttg aggatgatgg
     1201 attggaagga agaagacatt tgatttccga ttgccgattt tggattggtg attgcggggg
     1261 gattataact gatggttaat ggggattcac cctccatctt atggtgcgct ataggtttta
     1321 caagatattg gaataatacc aagtagtcta ttttatggga ctacatcgct agcgcaacgg
     1381 aaaccaatat gttcatagcc ttggtcagta gaataccacc aatgacgcgt aaaaactttt
     1441 aatggttcca aactattata gaaagttgaa acactccccc aaacccaagc tccaccacgt
     1501 acaactcgat tttctccgtc ttctggtcct aaagggttag cataagggga agattgatag
     1561 tatgttgcag agtaccaatc ccaaacccat tcttccacat taccacccat atcatatgca
     1621 ccatatggac ttttatcatt tagaaaactt ccaacagcaa ctgtatcatt ccccgcacaa
     1681 ccagaataat tagtgttaat acaattaagg tcttcccccc aaggatatgt tcgtccatca
     1741 ataccgcggg ctgctttttc ccattctgcc tctgtaggta atctaacttt ccgccaacta
     1801 caataagtat cagcctgtaa ccaactaaca ttcactacag gatagtcatt atagtttggg
     1861 ttgtcaaaat aattagaccg tgtagatgag ttcgtaaatt ctgggagagt acatgctcct
     1921 atttcaacgc aagttttgta ctgtgcattt gttacttcgt atttatcaat gtaataatcg
     1981 tccaaataaa caatatgtga aagattagca tcctgattcc cattgttact attgtcaatt
     2041 cccattgaaa actcacctgc tggtacaagc accattgaca cacctttggc atccgtgatt
     2101 tcggtaggca aaagtgtagg ttttatcgta gactctaagg ttgatgcagc aactgtctct
     2161 gtatatggtg aagtggtagc aacttgaggc gtcttttctt cggttgctgg ggcaatcatc
     2221 tgaaagaacg gcttccacat caaagctatt gcgccaaata caaaaatact ccaaaatata
     2281 agaactgtat taccaaaaag ttttctgatt gttagttttt gtcttggttt cttaatggtt
     2341 tcataaattt gttgttcagg tgatcttttt tcttttgatt tctgctcaac gggctcgttg
     2401 gatttgcgat ctgtcttacc aaggacaaga tattcctctt caacatcctt cttggaaatc
     2461 tcaaccgttg atcgttccaa ctgcgaagca cggaacttca gcgccttcat catcttggca
     2521 tagccagatt caaccgtcaa atccacccat tgataacggc tcaaggtaaa tgggacttca
     2581 cattcctcaa acttgacggg aatcaggaaa atccgtccct ccggcatttc gagggcttta
     2641 tccaaggcaa atttgatttc cttttggatg tagccttctt tatctactga gttcttggtc
     2701 aaacaaatga cgatggcgtc cgaagttgcc agggctttgg ggatttcaac ttgccagtcc
     2761 tgcccaccca ccaagtcctc ttcgtcaaac cacggttgaa tccctcgctt ttttaggtag
     2821 cgatagagtt cacggacttt gggcttgtcc gtggatgcgt ggcagaggaa gactttgaga
     2881 gggcgtttgg gttcaggcat aggaagggtt tcccgctgtg aattagtgaa attatacggc
     2941 agaatctgct atactcacac tatccgacaa aaaaggagtg gaaaatggat aaatatttac
     3001 ttcccgaaga tgatggttta cctgctcgaa cttttggtaa atggacaact gctaaaacag
     3061 attaccttca aagatatgtt gatctctttg aaacttcaat gcgcggtaaa ccttggtgcg
     3121 caagatgtta tattgacctt tactcaggag caggaaagta ccaagttgag ggacaaactg
     3181 gtttccatct tggttcccca ttaattggaa ttacaaccaa atatcctttc acccattatt
     3241 tctttgcgga tatttcgcaa gaaaatatcg atattttgaa acaacgttgt gaacccgcaa
     3301 gtcaaataag aaagaaattt tatgtaaggg atgccaattt acttgctcaa gaaatcgtta
     3361 atgaaattaa atccattgag cgaaatcgct caaaagaaca gtggcctctt taaatctggc
     3421 atttcttgac cccaatgggc tggaattaga atggtcaaca attgaatctc ttgcttcggt
     3481 gaataaaatg gatttaataa tttactattc acaaagtggg ttgacgagaa actttgaaaa
     3541 ttgtattaat gtagatggcg atactgacat tgatcgcttt tttggggaca gggaatggcg
     3601 ggatattttc aaaacatctc acgggaagag cgctggttta cacaggaaac tgattgatca
     3661 atacaaacat aaactatcca ttcttggtta tctcgatgtc aaggatattg aagatggcgc
     3721 agaaccatta atgaagaact ccaaagacgc tccgctgtac cgtcttattt ttgccagcaa
     3781 acacgcaaga ggacatgatt tctggaacaa aatcataaag aaggatgctt ttgggcaagg
     3841 aaaactattc tagaaaatag aaaattcgtg ctattattaa gtaaacagtc tttacaggta
     3901 aattcttcaa aggaggaaga atggctgaac attcaggaat cgagtggaca gaatccactt
     3961 ggaatccttt gacgggctgc accaagatca gtcccggctg caagcattgc tacgccgaac
     4021 gcatggcaaa gcgtctgcaa gcaatggggc aacccaatta ccgcaacggt ttcaaactca
     4081 ccctgcacga aaacgccatc gaaaaaccgc tcgaatggaa aaagccgcag gtcatctttg
     4141 tcaactcaat gagcgacctg ttccataaag atgtccctgt tgaatt
//
LOCUS       DAOQJE010000203         4180 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_12936_length_4180_cov_6.114182, whole genome shotgun sequence.
ACCESSION   DAOQJE010000203 DAOQJE010000000
VERSION     DAOQJE010000203.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 4180)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4180)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4180
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12936_length_4180_cov_6.114182"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..203
                     /locus_tag="PLT08_06770"
     CDS             <1..203
                     /locus_tag="PLT08_06770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE04201.1"
                     /translation="YGAHRGVTMGACILIAQGMHPLEAMELIKSKRPQADPDAFHIRP
                     RILKFAKEWQFMHHKDLKTQSR"
     gene            255..1202
                     /locus_tag="PLT08_06775"
     CDS             255..1202
                     /locus_tag="PLT08_06775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE04202.1"
                     /translation="MKRQAILTLLVLMTACTPQTTPLPTQRAIDTPTRTVPTPRYDIP
                     PQEPITVTAESTQTKQPTSMPVGEATQVTAADEDNAWMSLPVQPEPGVNIQDIYQLGL
                     TLGNDPHAFSIFGDCQSRPAEFFGVYETDAALIASLSPELQETVANFSGSFNRESPTS
                     QDGTTPGALLWDQWHRGEYGCQFGETPVDCELRIHRPSFVIIQIGSHFESRNTEYLRR
                     VIDQLLANGVVPILATKADNREKDNRINRDMAMLSAEYDLPLWNFWAAVDDLPNRGLY
                     TRKDRPLQGDIYLTDDAKLIHRETGLEALDIVWRMATGE"
     gene            complement(1298..1540)
                     /locus_tag="PLT08_06780"
     CDS             complement(1298..1540)
                     /locus_tag="PLT08_06780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE04203.1"
                     /translation="MSIDDAIKKMSDLVTAACASAEIKATKMSDEEARLTVLAPAGDM
                     QKIKDATFQPAIDMLNTDGMDVQVFVYDKDAPPLKG"
     gene            complement(1644..4043)
                     /locus_tag="PLT08_06785"
     CDS             complement(1644..4043)
                     /locus_tag="PLT08_06785"
                     /inference="COORDINATES: protein motif:HMM:TIGR01069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HNE04204.1"
                     /translation="MDSKTLNVLEYPKILDKLAGYCDFSASMALARELDPTDSYDLAI
                     ARLAETTEARKLLSVQDVSIGASHDIRQAADLAARSGVLDPQALLDVKSTLIAARELK
                     KTFEKKEDEYPRLCLIAEGLPETYGIVDAISRVLSERGEVLDSASVKLADIRRNLRIA
                     HDRLMSRLQKYVTDSKTSSMLQESIITQRDGRYVIPLRAEFKGKIKAVIHDQSSSGAT
                     LFVEPLPVVEANNEIRELQLAERDEERRILAEVSSVVGAHASELKYGVENLAVLDLAF
                     AKAKYSEELRASEPVMHERGKKNDASASLPILRLIQARHPLLDQDTVVPVDVDPREGT
                     RAIVITGPNTGGKTVSLKTVGLLVLMAQSGLHIPAQSGSELPFFKSVYADIGDEQSIE
                     QSLSTFSGHITNIIRILKQIDNRSLVIFDELGSGTDPQEGAAIARAILSHLLEVGVMT
                     LVATHYPELKTFAHSTEGVVNASLEFDIKTLRPTYKLTIGLPGRSNALAIAQKLGLPQ
                     PIIDSAKAEINPLDLRADKLLDDIRKERNRTSREREKLEKQRSKLEAQNADLAKRLEK
                     IEDERRETLAKARAEGELEVALLKRNIDSLKSQLKKASQPLTAIRAIEERMEKLEEKV
                     EAPVERKSEQSAVTSNQGLKLGEKVTVSSLNAEGVVTALGESDAEVQIGTIRVRAKMS
                     DLIRKSQEKSEASNEKKVVSKSVSSSRSSLPATSSPGMEVDLRGLMAEDALDKMERYL
                     EQAYLSGLPWVRIIHGKGTGKLRQAVRETLKGHTYVKSFEEGASNEGGEGVTVAKLNA
                     A"
BASE COUNT          976 a         1145 c         1040 g         1019 t
ORIGIN      
        1 cttatggcgc tcaccgcggc gtgaccatgg gagcttgcat cctcatcgcg caaggtatgc
       61 atccgctcga agcgatggaa ttgatcaagt ccaaacgtcc gcaagccgac ccagatgcct
      121 ttcacatccg tccgcgcatc ttgaagtttg ctaaagagtg gcaattcatg caccacaaag
      181 acttgaagac acaaagccgc taagattttc ttagtgactt cgagctttcg tgcctttgtg
      241 gttaaatcat cagaatgaaa cgacaagcca tcctaacctt attggtttta atgactgcct
      301 gcaccccgca aacgaccccg cttccgactc agcgggcaat cgatacgccc acccgaaccg
      361 ttcctacacc acgatatgat attcccccgc aagaacccat cacagtcaca gcagagtcaa
      421 cgcagaccaa acaacccacg tccatgccag tgggtgaggc gacacaggtc acagcggcgg
      481 atgaagataa tgcgtggatg agtctcccgg tccagccgga gccgggggtg aatattcaag
      541 acatctatca actcgggctg actctcggca atgatccgca tgccttctcc atcttcggtg
      601 attgtcaatc tcgccccgct gaatttttcg gcgtctatga aacggatgcg gcgctcatcg
      661 ccagcctctc acctgaattg caagagaccg ttgccaactt cagcggctcc ttcaaccgtg
      721 aaagccccac ctcacaagat ggcaccacgc ccggcgcctt gttatgggac cagtggcatc
      781 gcggcgaata tggctgtcaa ttcggcgaaa cgcctgtaga ctgtgaattg cgcatccatc
      841 gcccgtcttt tgtcatcatt caaattggtt cacacttcga gtcgcgcaac accgaatact
      901 tgcggcgcgt catcgaccag ttgctggcga acggcgtcgt tcccatcctc gccaccaaag
      961 ccgacaaccg cgaaaaagac aatcgcatca accgtgatat ggcaatgctc tccgccgaat
     1021 atgacttgcc cttgtggaac ttctgggcag cggtggatga cctgcccaac cgcggacttt
     1081 acacccgcaa agaccgtccg ctgcaaggcg atatttatct gaccgacgac gccaagctca
     1141 tccaccgcga aaccggcttg gaggcattgg acatcgtctg gcgcatggca acgggagaat
     1201 aaaaagtagg ggcgatgctt gtgccacgcc cactgtatcg acaaataaaa aggacgcgga
     1261 aaccgcgtcc ctacaatcat cattcgaaat tcagcactca gcctttaagg ggcggggcat
     1321 ctttatcgta cacaaaaacc tgaacatcca tcccatcggt attgagcata tcaatggcag
     1381 gctgaaaggt cgcatctttg atcttctgca tatcgcccgc aggtgcaagc accgtcaggc
     1441 gtgcttcttc atcagacatt ttcgtggctt tgatctcggc actggcacag gcggcggtta
     1501 caaggtcgga catttttttg atggcatcgt caatggacat aaacgtctcc tccataaaaa
     1561 ttataaacgc aatcataacc cggtcagatt acgattgcgt taagcgtaat acctaaaagt
     1621 actaagcaca atacattaaa cccctacgct gcgttcagct ttgccaccgt taccccttcg
     1681 ccgccttcat tagaagcccc ttcttcaaaa gacttcacgt aggtatgtcc tttcagggtc
     1741 tcgcgtacag cttggcgcag tttcccggtc cctttgccgt gaatgatgcg cacccaaggc
     1801 aaacccgaaa gatatgcctg ctctaaatag cgttccatct tgtcgagcgc atcttccgcc
     1861 atcaacccgc gcaagtccac ttccatgccg ggagaagaag tagcaggtaa cgaagagcga
     1921 gatgacgaga cactcttgct tacaactttc ttctcattac ttgcttctga tttttcctgt
     1981 gacttccgaa tcaaatccga catcttcgcc cgcacacgaa tcgtcccgat ttgaacttcc
     2041 gcatccgact cacccaaagc tgttacaaca ccctcggcat tcaagctgct gacggtgact
     2101 ttctcgccca gcttcaaccc ttggttactg gttactgcag actgttcact ttttctctca
     2161 accggtgctt ctaccttctc ttcaagcttc tccattctct cttcaatggc acggatggcg
     2221 gttaggggct ggctggcttt cttcaattga gacttcaacg agtcgatatt gcgtttgagc
     2281 aacgccactt ccaactctcc ttcggcgcgg gctttggcga gagtttcgcg gcgttcatct
     2341 tcgatctttt caaggcgttt tgccaggtct gcattttgcg cttcgagctt ggagcgttgc
     2401 ttttccagtt tctcacgttc gcgcgatgta cggttgcgtt ctttgcgaat gtcgtccagc
     2461 aatttatcgg ctcgcaaatc cagcgggttg atctctgcct tggcagagtc aatgatgggt
     2521 tgggggagcc ctaacttctg agcgatggct aaggcgttag acctgcccgg cagaccgatg
     2581 gtcaacttat aggtcgggcg aagcgttttg atgtcaaatt ccaaagaagc attgaccacg
     2641 ccttcggtcg aatgagcgaa ggtttttaat tcagggtaat gtgtcgcgac caaggtcatc
     2701 acgcccactt cgagtaaatg gctcaagatc gcgcgcgcga tggcagcgcc ttcttgcggg
     2761 tcggtgccgg agccaagttc atcaaagata accagtgaac gattatcaat ctgtttgaga
     2821 atgcggataa tgttggtgat atgtccactg aaggttgaaa gcgattgctc aatcgattgt
     2881 tcatcgccaa tatcggcata gaccgatttg aagaatggca actccgagcc gctttgggca
     2941 gggatatgaa gtccgctttg cgccatgagc acaagcaaac caactgtctt taatgaaact
     3001 gtcttaccac ctgtattcgg acccgtgatg acgatggcgc gtgtcccttc gcgcgggtca
     3061 acatctacag ggacgaccgt atcttggtcg aggagtgggt ggcgggcttg aatgaggcgt
     3121 aagatgggta gtgaagcaga ggcatcattc tttttacctc tttcatgcat tacaggttca
     3181 ctcgctcgta actcttcaga atactttgct tttgcaaatg ccaggtccaa cacggcgaga
     3241 ttttccacgc cgtatttcaa ttcactggca tgggctccaa ccacagatga aacttcggct
     3301 aggattctgc gttcttcgtc tcgttcggca agttgtaatt cgcgaatttc attattcgct
     3361 tctacgacag gcaacggctc cacgaagaga gtcgcgccgc tcgaagattg atcgtgaatc
     3421 accgctttga tcttgccttt aaattcagca cgtaatggaa tcacataccg cccatcacgc
     3481 tgggtgatga tcgactcttg cagcatggat gaggtctttg agtcggtcac atacttctgc
     3541 aggcggctca tcaaccggtc gtgggcgatg cgcagattgc ggcgaatgtc tgctaatttg
     3601 acggaagcag aatccagcac ttcgccgcgc tccgaaagga cgcgcgaaat tgcatccacg
     3661 atgccgtagg tctcgggcag tccctctgcg atcaggcaca agcgcggata ttcgtcttcc
     3721 ttcttctcaa aggtcttctt caactcgcga gcagcaatca gcgtggactt tacgtctagt
     3781 aaggcttgcg ggtcgaggac gccgctgcgg gcagcgaggt ctgcggcttg gcgaatgtcg
     3841 tgcgaggcac cgatgctgac atcttgcacg gagagtaatt tgcgggcttc ggtcgtttcg
     3901 gcgagacggg caatggcgag gtcatacgaa tcggtcgggt caagttcgcg ggcgagtgcc
     3961 attgaagcag aaaagtcaca atagcctgcg agcttgtcga ggatctttgg gtattcgagt
     4021 acgttgaggg tcttgctgtc cataatggga gattatactt cggacgacgg acgatagacc
     4081 attgtctatc gtcaaacaaa ggagcaaact atgagactca aaaataaaac tgccatcatc
     4141 accggcggca catcgggtat tggcaaggcg acggcgttgc
//
LOCUS       DAOQJE010000204         4170 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_12975_length_4170_cov_5.120778, whole genome shotgun sequence.
ACCESSION   DAOQJE010000204 DAOQJE010000000
VERSION     DAOQJE010000204.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 4170)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4170)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4170
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12975_length_4170_cov_5.120778"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1226
                     /locus_tag="PLT08_06790"
     CDS             <1..1226
                     /locus_tag="PLT08_06790"
                     /inference="COORDINATES: protein motif:HMM:NF014375.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="B12-binding domain-containing protein"
                     /protein_id="HNE04205.1"
                     /translation="LPTPQRTAGGHRLYSQYDIETIKWLIARQAEGLSISRAVDLWNE
                     QTASGSDPLAALAPMNMVLTQNVSTNIPADTTLDGIRAEWINACMNFSEVQAEQALNK
                     AFSIFPVEAVCVDVLQKGMSEIGSLWYENRASVQQEHFASGLAMRRLDALLSASPAPT
                     RAQTILMGCPSNEWHTFTSLLLALLLRRRGLNVIYLGANVPTERFSETAQKVKANLVI
                     LVAQTLNSAAHLQHAALSLSTQKVNVAFGGRIFNLRPSIVDYIPGHYLGATLEGSINE
                     VESLLKGVSKPRKIKAVAQEYVSAMQGFNSKRAHIESTLKLNMPPLSIGTEELDASIQ
                     HLGHNVSAALQFGDMEHLSEEMEWLKVLLHAYNRPKQELVYFMESYSNAIDAHINGQG
                     DPIKKWLRSYAVERT"
     gene            1231..2016
                     /locus_tag="PLT08_06795"
     CDS             1231..2016
                     /locus_tag="PLT08_06795"
                     /inference="COORDINATES: protein motif:HMM:NF018649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1295 domain-containing protein"
                     /protein_id="HNE04206.1"
                     /translation="MNFVSLWLTAGLIILVLMLLLWLYSLAIKNSSIVDIFWGTGFVI
                     TFWATAWIGATALSTRVLLLGVLVTLWGLRLSLYIFSRNHGHPEDFRYAKWREEAGSA
                     WWWRSFFKVFLLQGVIMWVIAIPLIAAQTSTPSPFKCECTDYTGAALWLVGFIFEAGG
                     DWQLARFKKDPANKGKLLTTGLWSLTRHPNYFGDAAQWWGFWLLAFANGAWWTFFSPL
                     IMTYLLMRVSGVTLLENTLTKTKPGYEEYMARTSAFFPWFPKK"
     gene            2098..3375
                     /locus_tag="PLT08_06800"
     CDS             2098..3375
                     /locus_tag="PLT08_06800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNE04207.1"
                     /translation="MNKRLLSIILVVFIDLLGFSLILPLLPYYAEKYGATQFVTGLLV
                     ASYAAMQLIGAPLLGRLSDRYGRRPILLASVFGTFLGFLLLGFADNIGTAMANAFDPQ
                     AANIFVLAILFVSRMVDGLTGGNLSVAQAYISDVTDAENRSKGLGMIGAAFGLGFIIG
                     PATGGILSQYGYAIPGFVAAALSFVNLSLIYFWLPESLTEEKRAEMTDKKPAVTLSAL
                     TKALTRPFTGALLTTRFFYGLAFSIFQTVFALYALQKFNFEARDTGFTLTFVGILSVF
                     VQGFLIGRLTKKFRDDNLILLGGVLMTISLLGWALTPNVFWLLIVLVPTAISGGILNT
                     LLSSTLTKAVQPQEIGGILGLGAAIESATRIFAPIIGGYLLQTFGTWSPGAFGAVMML
                     GITIFIYFTIYNHPIASEIRAKSIQQAPAPNGD"
     gene            3394..3972
                     /locus_tag="PLT08_06805"
     CDS             3394..3972
                     /locus_tag="PLT08_06805"
                     /inference="COORDINATES: protein motif:HMM:NF021577.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DICT sensory domain-containing protein"
                     /protein_id="HNE04208.1"
                     /translation="MNKDILGSFFNRILPLFPPRTEAVLKGMTTSTLEDMNFRYIFTV
                     DGMVTISHIIEEMINKNKGVADLHVSFQYFSRFADQEERYREVADHATGLWLYGVDDA
                     PLPALPRLTAVNTKDTPLENYWFVIAYGPGVSATLLAEEITPDHRLDNEPRMYEGFYT
                     FESDTGYQILMLMHQMFPNQVPLPVAPEKLRS"
BASE COUNT          986 a         1136 c          952 g         1096 t
ORIGIN      
        1 gtcttcctac cccgcaacgc acggcgggcg gacatcgctt gtactcgcag tacgatatcg
       61 agactatcaa gtggctgatc gcacgacaag cagaaggact atccatctct cgcgctgtgg
      121 atctatggaa tgaacaaacc gcctctggtt cggacccgtt agccgctctt gcacccatga
      181 acatggtgct cactcagaac gtatccacta acattcctgc tgatacgaca ttggatggca
      241 tccgcgctga atggattaac gcctgcatga atttcagcga agtacaagcc gaacaagcgc
      301 tgaataaagc cttctcaatc ttcccagttg aagccgtctg cgtggacgtg ttgcaaaagg
      361 gcatgtctga gatcggcagt ttgtggtacg agaatcgtgc cagtgtgcaa caagaacact
      421 tcgcttcggg tcttgccatg cgccgcttgg atgcacttct cagcgcctcc cctgccccca
      481 cccgtgcgca gacaattttg atgggctgtc cttccaacga atggcatacc tttacctcgt
      541 tattactcgc tttgcttttg cgccgccgcg gactgaacgt tatctatctc ggtgccaacg
      601 tccccaccga gcgcttctca gagaccgccc aaaaagtaaa agccaatctg gtcattttgg
      661 tggcgcaaac gttgaacagt gccgcacatc ttcaacacgc cgcactttcg ttgagcactc
      721 aaaaggttaa tgtagcgttc ggtgggcgga tatttaacct ccgccccagc atcgtggatt
      781 acattcccgg tcactacctc ggcgctacac tagaaggttc gattaatgaa gttgagtctt
      841 tgctcaaagg cgtttcgaag ccgcgcaaga tcaaagcagt tgctcaggaa tatgtctctg
      901 ccatgcaagg cttcaattcc aaacgtgctc atattgaaag cacgctcaaa ctgaacatgc
      961 ctccgctttc cattggcacc gaagaactgg atgcgagtat tcaacacttg ggtcacaatg
     1021 tgtctgctgc tttgcaattc ggagatatgg agcacctctc tgaagaaatg gaatggttga
     1081 aagttttgtt gcacgcgtac aaccgtccta aacaagaatt agtttatttc atggaaagtt
     1141 actcgaatgc cattgacgct catatcaatg gacaaggtga ccccatcaag aaatggctgc
     1201 gttcgtacgc agtagaaaga acctaacttt atgaatttcg tctccctttg gctgaccgcc
     1261 ggtctcatta ttcttgtcct catgcttttg ctgtggctgt acagcctggc aatcaagaac
     1321 tccagcatcg tggacatttt ctggggcact ggcttcgtca tcaccttttg ggcgacggca
     1381 tggataggcg caactgccct cagcactcga gtcctcctgc tcggtgtgtt ggtcactctt
     1441 tgggggctgc gcctatccct ttacatcttt tcgcgcaacc acggtcaccc cgaagacttc
     1501 cgatatgcca aatggcgaga agaagctggg tcagcttggt ggtggcgcag tttcttcaaa
     1561 gtttttcttc tgcaaggcgt catcatgtgg gtcatcgcca tcccattgat cgctgcgcaa
     1621 acctccacgc cttcgccgtt caaatgcgaa tgcactgact ataccggcgc tgcattatgg
     1681 ttggtcggct tcatcttcga agcaggcgga gattggcaac tggcacgctt caagaaagac
     1741 cccgcaaaca aaggcaaact gctcaccact ggcttgtgga gtctcacccg tcaccccaat
     1801 tactttggtg acgcggctca gtggtggggc ttctggctcc tcgcctttgc caacggagcc
     1861 tggtggacct tcttcagtcc cttaataatg acctatctct taatgagagt ttctggcgtg
     1921 acattgctcg aaaacacgct gaccaaaacc aaacccggtt atgaagaata catggcacgc
     1981 accagtgcat tcttcccgtg gttccctaaa aaataacgta tccacccttc aaaacgaaag
     2041 ccataacgca aagtcgacct ttgtgtggct tcgtgcaact tgaaccaaaa ggaaactatg
     2101 aacaaacgct tactctccat cattctggtc gttttcattg acttgctcgg cttcagtctt
     2161 atccttccgc ttttgcccta ctacgcggaa aaatacggcg cgacccaatt cgtgaccggc
     2221 ttactcgtcg cttcatacgc cgccatgcag ttgatcggcg cacccttact gggtcgcctc
     2281 tcagaccgtt acggacggcg ccccattctg ttggcatctg tctttggaac cttcctcggc
     2341 ttcctcctgc ttggctttgc agacaatatc ggcaccgcaa tggcaaatgc cttcgaccct
     2401 caagctgcta acatcttcgt attggcgatt ctcttcgtca gccgcatggt agatgggctc
     2461 actggcggga acttgagcgt ggcacaagcc tatatctctg atgtgaccga tgctgagaac
     2521 cgttcgaaag gtttgggcat gatcggtgcg gcattcggat tgggcttcat tatcggaccc
     2581 gctaccggcg gcatcttaag ccaatatggc tacgcaattc ccggctttgt agcggcggcg
     2641 ttgtctttcg tcaacctctc gttgatctat ttctggctac ctgaatcctt gaccgaagaa
     2701 aaacgtgccg aaatgacaga taaaaaacca gcggttacgc tttctgcatt gaccaaggcg
     2761 ctgacccgtc cattcaccgg cgcacttcta acaacgcgct tcttttatgg gctggcgttt
     2821 tccattttcc aaaccgtgtt cgcgttatat gctttacaaa aattcaactt cgaagcaaga
     2881 gataccggtt ttacactcac gttcgtaggc attttgtctg tttttgttca aggattcctc
     2941 attggcagac tgaccaagaa attccgtgat gacaacctaa ttcttttggg cggggttttg
     3001 atgacaatct cattacttgg ctgggcatta acacccaacg ttttttggct cttaatagtt
     3061 ctagtcccca ccgctatttc gggcggcatc ctcaatacac tcctatcctc cactcttacg
     3121 aaagccgttc aaccgcaaga gatcggcggc attctcgggt tgggcgctgc cattgaaagt
     3181 gccacacgta tctttgcacc gatcatcggc ggatatttac tgcaaacttt tggcacctgg
     3241 tctccaggag ctttcggggc tgtaatgatg ttgggcatta ccatctttat ttacttcacc
     3301 atctacaacc accccattgc aagtgagatc cgcgcaaaat cgatacagca agcgcctgcg
     3361 ccaaacggtg attaattctt aaaggacttt ttaatgaata aagatattct tgggagtttc
     3421 tttaaccgta tcctaccgct cttccctccg cgcacagaag cggtcttgaa aggcatgaca
     3481 acatcgacgt tggaagatat gaactttcgt tatatcttca ccgtcgatgg catggtgacc
     3541 atcagccaca tcattgaaga aatgatcaac aagaacaaag gtgtagctga cctgcatgtc
     3601 tcttttcaat atttttcgcg ctttgccgat caagaagagc gttaccgtga agtggcagac
     3661 catgctaccg gtctctggct gtatggcgta gatgatgctc cgcttcctgc tcttccacgc
     3721 ctgacggcgg tcaacacaaa agacactccg ctggaaaact attggttcgt gatcgcatat
     3781 ggccccggcg taagtgcaac cttgctcgct gaagaaatca caccagacca tcgcttagac
     3841 aatgagccgc gcatgtatga gggcttctac acttttgaat ccgatacagg ctatcagatc
     3901 ctcatgctta tgcaccagat gttccccaat caagtacctt tgcctgtagc gcccgaaaaa
     3961 ttaaggtctt agtcttttac aaaaggagcc attcatgtta atcgacacac tacgttatga
     4021 attaccgccc gaaggaatta ctcttttagg ttatgttgtg cgccgcatgc acaaaacaga
     4081 gtggctcgta aaagccagcg agatgttagc gaatgaaaaa acaagcgagg cacttgagtc
     4141 cgttgccatt tatgcagccg cctcctccac
//
LOCUS       DAOQJE010000205         4141 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_13114_length_4141_cov_5.346304, whole genome shotgun sequence.
ACCESSION   DAOQJE010000205 DAOQJE010000000
VERSION     DAOQJE010000205.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 4141)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4141)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4141
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13114_length_4141_cov_5.346304"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..871)
                     /locus_tag="PLT08_06810"
     CDS             complement(<1..871)
                     /locus_tag="PLT08_06810"
                     /inference="COORDINATES: protein motif:HMM:NF014762.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HNE04209.1"
                     /translation="MYYTIAAITLLVLIFMPRIGLLALYKNYRTSQIREQVEDALKHL
                     LDRERQGRHASPESLGGTLDLQRARVTHLIQDMEAQGLVESNGNELHLTAKGERWALH
                     VVRAHRLWERYLADEARMPLNKIHDEAQKREHSLTEAQLNELDAALGHPTRDPHGDPI
                     PTREGKMNQTEGKPITAWQADRPARIVHLEDEPALAYQQILAAGLRLGQDIRVIEKTP
                     NRIVLSDGENEYRLAPAVAYNIHVAAIPESEMLKREAVPLFELEHDQRGEIVTLDDAV
                     QGFTRRRFLDLGLT"
     gene            complement(887..1480)
                     /locus_tag="PLT08_06815"
     CDS             complement(887..1480)
                     /locus_tag="PLT08_06815"
                     /EC_number="2.1.1.100"
                     /EC_number="2.1.1.334"
                     /inference="COORDINATES: protein motif:HMM:NF016107.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenylcysteine carboxylmethyltransferase
                     family protein"
                     /protein_id="HNE04210.1"
                     /translation="MNETIFRILAAVILFTGVGISIYFRSKADRTSGEKLSRNVDGTP
                     LMLVIRIFGLVLWLSPLVYLVNPVWMNWSKISLPAWSRWFGVVFGIFCVFGIYWLFTS
                     IGSGITPVSATRKEHKLATSGIYRWIRHPLYTVGSSFIVSFGLIADNWFIIALGMLAF
                     TIMAIRTPKEEENLIEKFGDEYRDYMQRTGKFFPKFK"
     gene            complement(1541..2203)
                     /locus_tag="PLT08_06820"
     CDS             complement(1541..2203)
                     /locus_tag="PLT08_06820"
                     /inference="COORDINATES: protein motif:HMM:NF015956.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HNE04211.1"
                     /translation="MKTSTSIQDYLKRIYELTESGQSASTNDLARELKVKPASVTGMI
                     QKLAAETPALVEYQKHQGVTLTVAGKRAALEVIRHHRLLETWLVQTLGYSWDEVHEEA
                     ERLEHVISEDFERRIAAALGHPLRDPHGELIPTEDLKMPLDESTPLSALRKDQSGTIL
                     CIKAADADLLRHLTELGLTPNTFIQVTEYSPYDHNLTIRVDKKTHVLGLSITSKIFIE
                     EG"
     gene            complement(2207..2896)
                     /locus_tag="PLT08_06825"
     CDS             complement(2207..2896)
                     /locus_tag="PLT08_06825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE04212.1"
                     /translation="MELIPFNLLGILGIVGATTYAVGDVLLLASKISLDDYPKLKPFA
                     KLLSDSEKMVALSPIRMMWGALLGVFATPLVLFGFWHAYQGLSGVNAWVALIAFFLFA
                     LASIIGTFVHGTFYYMGEYVHALNKVSTDSQAVIVEMIERHKKVLIITYAPLLVFIIF
                     ASILFSILVASGNTLFPVWMTAINPLTLTILWMLGKRILPQFVRDWTEGAGFNIAYMA
                     FFACTTITLWK"
     gene            3093..>4141
                     /locus_tag="PLT08_06830"
     CDS             3093..>4141
                     /locus_tag="PLT08_06830"
                     /inference="COORDINATES: protein motif:HMM:NF012692.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family transcriptional regulator"
                     /protein_id="HNE04213.1"
                     /translation="MQKATHQQTKQHNRDLVLRTIFANESISRAEVARVTNLTRTTVS
                     DVVNSLLDEGLVEEVGRGESIGGKTPILLSVVADSRYLIGLNLAQDKFIGAIVNLRGE
                     IKEMVEVPVHGEDGKNALDLVYQIIDQLMKKRIKPIVGIGVGAPGLVNTREGVVVNAV
                     NLEWQDLPLGQLLEKKYKIPVLILNDSQATAIGEFVYGGAHALDENLIVVNVKHGIGS
                     GILVNGRLFQGDGGGAGEIGHVVVQENGELCRCGKHGCLETISSARAVLRQLNLKSLD
                     EAKSAFDAGDEKTQWVIKNTAHYLGISLANLIGILNIQKIIITGDMTRFGAPWMDEVN
                     NSMRNAALARLSENT"
BASE COUNT         1076 a          930 c         1016 g         1119 t
ORIGIN      
        1 gagtcagccc gaggtccaga aagcggcggc gcgtgaagcc ttgcacggca tcatccagag
       61 tcacaatttc gccacgctga tcgtgctcca attcaaataa cggaacggct tcacgcttta
      121 gcatctcgct ctcaggaatt gccgccacat gaatgttata tgccacagcc ggtgcaagcc
      181 gatattcatt ttcgccgtca ctcaacacaa tgcggttcgg tgttttttca atcacgcgga
      241 tgtcttgccc caagcggagc cctgccgcca ggatttgttg atacgccaga gcgggttcat
      301 cctctaagtg gacgattctt gccggacggt ctgcctgcca cgccgtaatg ggctttcctt
      361 ctgtctgatt cattttcccc tcacgcgttg gaatcgggtc gccatgtggg tcgcgagtgg
      421 ggtgacccaa tgcggcatcg agttcattta gttgtgcctc agtgagagag tgttcacgtt
      481 tctgcgcttc gtcatggatc ttgttcagtg gcatgcgtgc ttcatctgcc aagtaacgct
      541 cccataaacg atgtgcacgt acaacatgca atgcccagcg ttcgcctttg gcagtgagat
      601 gtaattcatt cccattcgac tcaaccaaac cttgagcttc catatcttga atgagatggg
      661 tgactctggc gcgttgaagg tctagggtgc ctcccaaaga ttctggagag gcgtgccttc
      721 cttgtcgttc acggtctaga aggtgtttca gagcatcttc cacctgctca cggatttgac
      781 tggtacggta atttttataa agcgccaata atcctatgcg cggcatgaag ataaggacta
      841 ataatgtaat cgcagcaatg gtgtaataca tagttttttt ccttgcttat ttgaatttcg
      901 ggaagaattt tcccgtccgt tgcatatagt cgcgatattc atcgccgaat ttctcaatga
      961 gattttcttc ttctttcggc gtacggattg ccatgatcgt gaatgccaac atacctaatg
     1021 cgatgatgaa ccagttatct gctatgagac caaacgagac gataaacgat gaacccacgg
     1081 tataaagtgg atgccttatc cagcggtaga tgccactggt tgcaagttta tgttctttac
     1141 gcgttgcact gactggtgta attccgctgc caatactggt gaacaaccag taaattccga
     1201 agacacaaaa tataccgaac acgactccga accaacggct ccatgcgggt aaactgatct
     1261 tcgaccagtt catccataca gggttgacta agtagacgag cggacttagc cagagcacca
     1321 acccaaagat gcgaatgacc agcatcagcg gagtgccatc tacattgcga gatagttttt
     1381 ctcctgaagt acggtctgcc ttgctacgga aatatatgga gatgcctaca cctgtaaaaa
     1441 ggataacggc tgccaagatt ctaaaaatgg tttcgttcat agtgttaaag cctttcttct
     1501 ttctgtttct tacttttcat ttattcgata actttgtcga ttaaccttcc tcaatgaata
     1561 tcttgctagt gatacttagc cccaacacat gtgtcttttt atcaacccga atggtaaggt
     1621 tgtggtcgta tggggaatat tctgtcacct gaatgaacgt gtttggtgta agccccagct
     1681 cggtcaggtg ccgaagcagg tctgcgtctg cggctttgat gcataagatc gtgccagatt
     1741 ggtcttttcg caaagcagat agcggagtgg actcatccag cggcatctta agatcttcgg
     1801 tggggatcaa ctctccgtgc gggtcacgaa gcggatgccc caaagcggca gcgatgcgtc
     1861 gctcgaaatc ctctgaaatg acatgctcca atcgttctgc ctcttcatgg acctcatccc
     1921 atgaataccc aagggtttgt accagccagg tttccaataa acgatgatga cggatcactt
     1981 ccagagcggc acgtttgcca gccacggtga gagtgacgcc ctgatgcttt tggtattcga
     2041 ccaaggcagg cgtttctgct gcaagttttt ggatcatacc agtgacagat gctggcttga
     2101 cctttagctc acgcgccaga tcattggtgc tggctgattg cccgctttcg gtcagttcgt
     2161 agatgcgttt gagataatct tgtatggatg tggatgtttt cattgattat ttccaaagag
     2221 tgatagttgt gcaggcaaag aatgccatat atgcgatgtt gaaaccggca ccctccgtcc
     2281 aatcgcggac gaattgcggc agaatgcgct ttccgagcat ccaaagaatc gtgagggtaa
     2341 gtgggttgat cgctgtcatc catactggga ataaagtatt cccagaagcg accaaaatcg
     2401 aaaataggat agaagcgaaa ataatgaaca ccaacaacgg ggcatacgtg atgataagaa
     2461 cttttttgtg tctttcaatc atttctacga ttactgcttg cgagtcagtg gagactttgt
     2521 tcaaggcatg cacatactcg cccatgtagt aaaacgttcc atgtacaaag gttccgatga
     2581 tggaagccaa tgcaaaaagg aaaaaagcga ttaatgctac ccaggcgttg actccgctta
     2641 agccttgata tgcatgccaa aacccaaata gtacgagggg agttgcaaac actccaagta
     2701 atgcgcccca catcatgcgg atgggggaga gtgccaccat tttttctgag tcagaaagta
     2761 gtttggcaaa tggcttgagt tttgggtagt cgtcgaggga gatttttgag gcaagtaaaa
     2821 gcacatcacc aacggcatag gtggtcgcac caacgatgcc cagaatacca agtaagttaa
     2881 agggaataag ttccataatt ttcgtttata aaataattta gtcttaccta aattatttta
     2941 taatatcccc taaaaaatgt caatagattc agccttgact cttgctgcgg aaatctgtat
     3001 actaactttg ttagtacagt gaatttacaa agtaattaaa tgatcgtttg ctatccaaga
     3061 tacttctgcg cgtagaaatt ttggcaaaaa tcatgcaaaa agcgactcat cagcaaacca
     3121 agcaacacaa tcgtgatctc gttctgcgaa ccatcttcgc aaacgaatct atcagccgtg
     3181 cagaagtggc gcgtgttacc aacctcacac gcaccacggt ttcagatgta gtcaacagtt
     3241 tgttggatga aggcttggtc gaagaagttg gcagaggcga atcgattggt ggcaaaacac
     3301 cgatcctgct tagtgttgta gcagattcgc gttatctgat tggtttgaat ctcgcacagg
     3361 ataaattcat tggcgcgatc gtaaatttac gtggggaaat caaagaaatg gtcgaagttc
     3421 ccgttcacgg ggaagatggc aagaatgccc ttgacctcgt ttatcaaatt attgatcaat
     3481 tgatgaagaa acgcatcaag cccatcgttg gaattggagt gggcgctccg ggcttggtca
     3541 acacccgcga aggtgtggtg gttaatgccg tcaatttgga atggcaagat ttgccacttg
     3601 gtcaattgct ggaaaagaaa tacaagatcc ctgttttgat cttaaatgac agccaggcaa
     3661 ccgctattgg tgagtttgtc tatggtggcg cgcatgcttt ggatgaaaac ttgattgtag
     3721 tcaatgtgaa acatggcatt ggctctggta ttttagtcaa cggtcgtttg ttccagggcg
     3781 atgggggcgg ggcgggcgaa attggtcatg tggtggtgca ggagaatggt gaattatgcc
     3841 gttgtgggaa gcatggctgt cttgaaacca tttccagcgc tcgtgcagta cttcgccaat
     3901 tgaatttgaa atcgcttgat gaagccaaat ctgcgtttga tgcgggcgat gaaaaaacac
     3961 agtgggtcat caagaacacc gcccattatc tgggaatctc tctcgcgaat ttgatcggta
     4021 ttttgaacat tcaaaagatc atcatcaccg gcgatatgac ccgctttggc gctccgtgga
     4081 tggatgaggt caataactcc atgcgcaatg cggcgttggc gcggctctct gaaaatacga
     4141 a
//
LOCUS       DAOQJE010000206         4140 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_13120_length_4140_cov_3.748837, whole genome shotgun sequence.
ACCESSION   DAOQJE010000206 DAOQJE010000000
VERSION     DAOQJE010000206.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 4140)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4140)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4140
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13120_length_4140_cov_3.748837"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..292)
                     /locus_tag="PLT08_06835"
     CDS             complement(<1..292)
                     /locus_tag="PLT08_06835"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: protein motif:HMM:NF013530.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HNE04214.1"
                     /translation="MTWLITGGCGFVGSNLADFLLSSKEDVVVFDNLSRAGSHENLNW
                     LQTKHGSHWRFIEADIRNEETVLKLIGEIRPKVIAHLAGQVAMTTSLQNPRDD"
     gene            complement(333..1268)
                     /locus_tag="PLT08_06840"
     CDS             complement(333..1268)
                     /locus_tag="PLT08_06840"
                     /inference="COORDINATES: protein motif:HMM:NF027681.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HNE04215.1"
                     /translation="MRGKKFTPQLAEFHSFFSKRRVIITGADGFIGGHLRDVLVSLNA
                     KVYGINRSAKANINNYKSISTDLSNLKDLQSAIDTIQPETIFHLAGKVTGRQNIELVY
                     PTLQDNLIGTINLLLASSNNTGIRRIITVGSAEETINSTPNSPYSVSKTAVALYTKMF
                     HQIYSLPIVTAKLFMVYGPHQQEDKLIPYSIISLLKGKFPQITSKDRLVDLIYISDVV
                     RGLLLTATYPGIEGQTFEFGTGESVKIKQIIKTLTSISGNATKIKAENPIHRIQEKNL
                     VANINKTKKILGWVPEWTLRDGLWETFNWYQKHLI"
     gene            complement(1299..2618)
                     /gene="rfbH"
                     /locus_tag="PLT08_06845"
     CDS             complement(1299..2618)
                     /gene="rfbH"
                     /locus_tag="PLT08_06845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017944024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide biosynthesis protein RfbH"
                     /protein_id="HNE04216.1"
                     /translation="MKKTTEELRKQILSLTAEYFTQAFQPREFIPGKSPIPVSGRVFN
                     EIELQHLVDSSLDFWLTTGRFADNFQKEFSKFMGVRNTILVNSGSSANLVAISCLTSP
                     QLGDKALRPGDEVITVAAGFPTTVNPIIQNNLIPVFIDIEIPTYNIDIQYLEAALSPR
                     TRAIMIAHTLGNPFNIDEVTKFAKKHNLWLIEDCCDAVGATYRNKQVGTFGDIATTSF
                     YPAHHITMGEGGAVLTDKPKLKTLAESFRDWGRDCWCEPGKDNTCGKRFEHQLGDLPH
                     GYDHKYTYSHVGYNLKATDMQAAVGLAQMTKISGFINARRENFTYLDKNLRELQSYLI
                     LPKATTYSNPSWFGYPIAVQQDSPFSRNQLIRFLENRKIATRLLFGGNLVRQPAYKNT
                     NFRVASTLKNTDFVMNQVFWIGVYPGLTRQMLDYVIESFHDAKSSLS"
     gene            complement(2602..3603)
                     /locus_tag="PLT08_06850"
     CDS             complement(2602..3603)
                     /locus_tag="PLT08_06850"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: protein motif:HMM:NF027681.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HNE04217.1"
                     /translation="METMVNRTFWLDRPVFVTGGTGLVGSWLVRRLVEAKADVVCLLR
                     DWVPQSELVRTGLIERVKVVHGDIRDRNLLERALGEFEIDTVIHLAAQTIVTIANRNP
                     ISTFETNIAGTWNLLEASHRSPKVRQIILASSDKAYGDQDKLPYSEDTPLQGRHPYDV
                     SKSAADLLAHTYAKSYELPVAITRCGNFYGGGDLNWNRIIPGTIRSILRGQEPVIRSD
                     GEYIRDYFYVEDGAAAYMLLAEQLASHPTLKGQAFNFSNEIQVTVKEIVESVIRLMGS
                     NLKPIILNEANNEIRHQYLSAEKARQLLKWEPLFNLDQGLALTIDWYKNFFSHEKNN"
     gene            complement(3588..>4140)
                     /locus_tag="PLT08_06855"
     CDS             complement(3588..>4140)
                     /locus_tag="PLT08_06855"
                     /inference="COORDINATES: protein motif:HMM:NF012695.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HNE04218.1"
                     /translation="SITVKLKTGDVTAHNIEGEDWIVHLLDTGIDTNTGGRVKRAAEF
                     IGNETFMLTYGDGVGNVDINTLIKFHKNHGKLATMTAVRPVARFGQIVLQGNQVVEFA
                     EKPQTGEGWINGGFFVLQPDIIKYIEDDQVIWERKPMERLASDGELMAYNHNEFWQCM
                     DTLRDVQLLNNLWDGGNAPWKLW"
BASE COUNT         1123 a          807 c          830 g         1380 t
ORIGIN      
        1 agtcatcgcg aggattttga aggctggttg tcattgcaac ctgtcctgca agatgtgcga
       61 ttaccttggg acggatttcc ccaatcaatt tcagtacggt ttcctcattt ctgatatcgg
      121 cttctataaa acgccagtga gagccatgct ttgtttgtag ccagtttaag ttttcgtgag
      181 agccagctcg acttaagtta tcaaagacaa ccacatcttc tttgctggat aataaaaagt
      241 ctgcaagatt actgcctaca aagccacaac cgcctgtgat taaccaagtc atacaaattt
      301 acctctgatt attgttttat ttattaaggg ttttatatta aatgcttttg ataccaattg
      361 aatgtttccc aaagcccatc ccgtaatgtc cattctggca cccatcctaa gatttttttt
      421 gttttattta tattggctac gagattcttt tcttgaatac ggtgaatagg attttctgct
      481 tttattttag tggcatttcc agaaatagat gttagggttt ttattatttg ttttattttg
      541 acactctctc cagttccaaa ttcaaatgtt tgaccttcta tacccggata agttgctgtt
      601 agtaaaagcc ctcgaactac atctgatatg tagattaaat ctaccagacg gtccttgctt
      661 gtgatctgag gaaattttcc ttttagaagt gaaataatgg agtatggaat tagtttgtct
      721 tcttgttgat gcggaccgta aaccataaaa agttttgctg ttactattgg taaagaatat
      781 atttggtgga acatttttgt gtataacgca actgctgttt ttgagactga gtatggggaa
      841 tttggggtgc tgtttatagt ttcttctgca gagccgacag ttattattcg tctaatccca
      901 gtattattag atgatgcaag taaaagattg attgtgccaa tgaggttgtc ttgaagcgtt
      961 ggatatacca attcgatgtt ttgcctacct gtgaccttac ctgcaagatg aaagatagtt
     1021 tcaggttgta ttgtgtctat tgctgattgt aaatctttaa gattgcttag gtctgtgctt
     1081 atgcttttgt aattatttat gtttgctttc gcacttctgt taattccgta cacttttgcg
     1141 tttagagaaa caaggacatc tcgtaggtgc cctccaataa acccgtctgc gccggttatt
     1201 attactcttc tttttgaaaa aaaagaatgg aattcagcta actgtggagt aaattttttg
     1261 cctctcaatt tattgacaat cctttttgtg aaaataaatt aacttagtga cgattttgcg
     1321 tcatgaaaac tttcaattac ataatccagc atttggcgag ttaagccagg atagacgcca
     1381 atccaaaaaa cttgattcat aacaaagtct gtatttttta gtgtgctggc aacgcgaaaa
     1441 tttgtatttt tgtaagcagg ttgcctgaca aggttcccgc cgaagagtag gcgagtagca
     1501 atttttctgt tttcaagaaa tcgtatcaat tgatttcgtg aaaagggcga atcttgctga
     1561 acagcgattg ggtaaccaaa ccaacttgga ttgctgtatg tagttgcttt tggaagaatt
     1621 aaataacttt gtaattctct gaggtttttg tctaaataag taaaattttc acgccttgca
     1681 tttatgaaac ctgaaatctt tgtcatttgt gcaaggccaa cagctgcttg catgtcagtt
     1741 gcctttaagt tatagcctac gtgtgagtag gtgtatttat gatcgtatcc atgtggtaaa
     1801 tcacctagtt gatgttcgaa tctttttcca caggtattat cttttccggg ttcacaccaa
     1861 caatcacgac cccaatcacg aaaagactcg gccaaagttt tcaattttgg tttatcagtt
     1921 aaaacggctc ccccttctcc catggtgata tgatgtgctg gatagaaact tgttgtggca
     1981 atatctccaa aggtgccaac ttgtttattt cgataggttg ctccaacggc atcacagcaa
     2041 tcttctatta gccagaggtt atgttttttt gcgaatttag tgacctcgtc aatgttgaaa
     2101 gggttgccaa gtgtgtgtgc aatcattatc gcacgcgtac gtggacttaa cgctgcttct
     2161 aaatattgaa tatctatatt gtatgtcgga atttcaatat caataaaaac aggaattaga
     2221 ttgttttgga ttattgggtt tacggtggtg gggaaaccgg cggccacagt gattacttca
     2281 tctccaggac gaagagcttt atctccaagt tggggggatg taagacagga aatagccaca
     2341 agatttgcag aggagccaga atttaccaaa atagtgttcc gaacccccat aaatttagag
     2401 aattcctttt ggaaattatc tgcaaaacgg ccagtggtca gccagaaatc taaagacgaa
     2461 tcaacaaggt gttgtaattc gatttcattg aataccctgc ctgaaacagg tatgggtgat
     2521 ttgccaggaa taaattcgcg cggctgaaac gcctgcgtga agtattcggc tgtaagcgaa
     2581 agaatttgct tgcgaagttc ttcagttgtt tttttcatga ctaaaaaaat ttttatacca
     2641 atctattgtt aaggctaaac cttggtcgag attgaataat ggttcccatt ttaaaagttg
     2701 ccttgctttt tctgcgctca agtattgatg acgtatttcg ttgttagctt cgtttaggat
     2761 gataggcttg aggtttgatc ccattagtct tataacactt tcaacaattt ccttgacggt
     2821 cacttgtatt tcatttgaaa aattaaatgc ttgacctttg agtgttgggt gagacgcaag
     2881 ttgttctgct aataacatat aggcggctgc accatcttcg acataaaagt aatcgcgaat
     2941 atattcgcca tcagaacgaa tcacgggttc ttgacctcgt agtattgaac gaattgtgcc
     3001 tgggatgatt cgattccagt ttaaatctcc gccgccataa aaattcccgc aacgagtaat
     3061 tgcaactggt aactcataac ttttggcata ggtatgcgca agtaaatctg ctgctgattt
     3121 acttacatcg tatgggtgac ggccttgtaa aggagtgtct tcgctgtaag gtagtttatc
     3181 ttggtcgcca tatgccttat ctgaagatgc caatatgatt tgacgtactt tagggctgcg
     3241 atgggaggct tcaaggagat tccacgttcc tgctatgtta gtttcaaaag ttgaaatcgg
     3301 gttacgatta gcaatagtta caattgtttg tgcagccaaa tgaatgacag tgtcaatttc
     3361 aaactctcca agtgcgcgtt ccagcaaatt gcggtcgcga atatcgccat ggactacttt
     3421 tactcgttca attaagcccg tgcggacaag ttcactttgc ggcacccagt cacgtaaaag
     3481 gcacactaca tctgcttttg cttccactaa ccttcgaaca agccaggacc ccaccaaacc
     3541 agttccaccg gttacaaaaa cagggcgatc cagccaaaat gtccgattca ccatagtttc
     3601 catggagcgt taccaccatc ccaaagatta ttcaagagtt gcacatcgcg caatgtatcc
     3661 atgcactgcc agaactcatt atggttgtat gccattagtt caccatcaga tgccagcctc
     3721 tccattggtt ttcgttccca aattacttga tcatcctcaa tgtatttgat gatatcaggt
     3781 tgtaatacaa aaaatcctcc attaatccag ccttcgcctg tttgtggttt ttcagcaaac
     3841 tctacaactt ggtttccctg tagaacaatt tgtccaaaac gtgctactgg gcgtactgcg
     3901 gtcatggtag ccaatttgcc atggtttttg tgaaatttga ttaatgtatt aatatctaca
     3961 ttacctacac catcaccata tgtaagcatg aaggtttcgt ttccaatgaa ttctgctgct
     4021 cgtttaacgc gaccacctgt gtttgtatca attccagtat ccaataaatg aacaatccaa
     4081 tcttctcctt ctatattgtg tgcggttaca tcgcctgttt taagcttgac tgtaatactc
//
LOCUS       DAOQJE010000207         4138 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_13125_length_4138_cov_6.036003, whole genome shotgun sequence.
ACCESSION   DAOQJE010000207 DAOQJE010000000
VERSION     DAOQJE010000207.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 4138)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4138)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4138
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13125_length_4138_cov_6.036003"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1225)
                     /gene="lysS"
                     /locus_tag="PLT08_06860"
     CDS             complement(<1..>1225)
                     /gene="lysS"
                     /locus_tag="PLT08_06860"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HNE04219.1"
                     /translation="KIQLFLRVNEVGEDRLAFFNKMFDLGDFVEATGIMMRTKAGEAS
                     LQVLDFNLLAKSISPLPADKDVTQEDGTVVRYASLDDVELRARQRYADLAVNPDVRDT
                     FRKRAKLIKSLRTFLDDHNFLEVETPILQPLYGGAAARPFVTHHNELDQDMYLRISFE
                     LYLKRLLVGNLERVYEIGRDFRNEGVSFKHNPEFTQLEFYWAYADYLQVMELTEQMVA
                     YAAEQVTGSTKVTFGEHELEFKPPWKRVEMRDGILETSGIDIAEHKTAEALFKAIKEK
                     LPKAMPDPKSTRGKLIDFLVSEYLEPTLIQPTFLYNYPRDISPLAKSMPGDPLTVERF
                     EGYVAGFELCNAFTELNDPLDQEQRFLEMGRDYENDDEEKHPLDEDYLRAMRYGMPPN
                     GGFGMGVDRLTMVLTN"
     assembly_gap    1226..1325
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(1379..1849)
                     /gene="greA"
                     /locus_tag="PLT08_06865"
     CDS             complement(1379..1849)
                     /gene="greA"
                     /locus_tag="PLT08_06865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HNE04220.1"
                     /translation="MPTSFLTKEGYDKLQEELDHLRTVKRQEVAERLHEAMEGGELIE
                     NAEYEAAKNEQAFVEGRIQELDLLLATAQIIEDNGKGKKHDAIQVGSKVTIKEGNYEA
                     ETFTIVGAAEANPREGKISNESPIGKAILGHKVGDTVKVETPGGTYNVKILKVA"
     gene            complement(1992..2717)
                     /locus_tag="PLT08_06870"
     CDS             complement(1992..2717)
                     /locus_tag="PLT08_06870"
                     /inference="COORDINATES: protein motif:HMM:NF014625.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HNE04221.1"
                     /translation="MRWIYLSPHLDDAALSAGGWIYDQTQAGNKVEIWTFLCGYPPSK
                     ELSMFARILHMQWGMPTARQVIRMRRAEDKAAATILGAKVRHFDFLDCIYRRDVEGNW
                     LYTDVFVDPHPSEANLPAEIASAISARLKSDDKLICQFGVGKHVDHVTVRRAAELLGR
                     PLHYVTDIPYYFNTPDHLAPNTVGMKKTVERVSRDGLASWKEAILAYKSQISSLFKSQ
                     RAMRAQINQYCSEFKGMPFWTTN"
     gene            2794..3183
                     /locus_tag="PLT08_06875"
     CDS             2794..3183
                     /locus_tag="PLT08_06875"
                     /inference="COORDINATES: protein motif:HMM:NF018894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HNE04222.1"
                     /translation="MQERRKEERKNLVAYTQVYDLYGGNLLGYLGDLTISGAMIISEK
                     AMKPDTEITLAIELPEMPGIKAIRMSLLTRVAWCEQDLSPQYFNIGFEFKEITPDQKG
                     LIEAIIKNYEFRRDVPKYPTRGAPPKQ"
     gene            3240..>4138
                     /locus_tag="PLT08_06880"
     CDS             3240..>4138
                     /locus_tag="PLT08_06880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase"
                     /protein_id="HNE04223.1"
                     /translation="MFPAKTLFFTAPRQVEVRESNLPLLKEDEVLVETICSAISAGTE
                     MLVYRDQFPQLKDSHDAVSSELKYPLAYGYACVGKVREIGKVVGKEWQDRLVFSFQPH
                     TSHFVSKPENLFPIPNSLKPENACFLPNMETAVNLVQDGAPILGERVLVLGQGIVGLL
                     TASLLHEFPLENLTVVDAIELRRKAMKEIEGQMSKVNKLSPSDLQSSSFDLVFELTGS
                     PSALNTAIEHTSFSGRIVIGSWYGQKRAEIDLGGSFHRSRIQLISSQVSSISPTLSGR
                     WDKSRRFEVAWQALERIQPQKWIT"
BASE COUNT         1002 a         1008 c         1002 g         1026 t
ORIGIN      
        1 tattcgtcag caccatcgtc aaacggtcca cgcccatgcc gaagccgccg ttgggcggca
       61 tgccgtagcg catggcacgc aggtaatctt catcgagcgg atgcttctct tcgtcatcgt
      121 tttcgtagtc gcgtcccatt tcgaggaagc gttgttcctg gtcaagcgga tcgttcaact
      181 cggtgaaggc attgcacaac tcaaagcctg ccacatatcc ttcaaaacgc tctaccgtga
      241 gcgggtcacc gggcatggac tttgccaacg gcgaaatatc acgcgggtag ttatatagaa
      301 aagtcggctg gatgagggtc ggctcgagat attcgctgac gaggaaatcg atcaacttgc
      361 cgcgagtgga cttcggatca ggcatcgcct tgggcagttt ctctttaatt gccttgaaca
      421 acgcctcagc cgttttgtgc tcggcgatgt cgataccact ggtttcaaga atgccgtctc
      481 gcatttccac gcgtttccac ggcggtttga attcgagttc gtgttcaccg aatgtgacct
      541 tggtggagcc ggtcacctgc tcggcggcat aagccaccat ctgctcagtg agttccatca
      601 cctgcaaata gtccgcgtat gcccaataga attcaagctg tgtaaattcg gggttgtgct
      661 tgaaagacac gccttcgttg cggaagtcac gtccgatctc atagacacgt tccagatttc
      721 ccacgagcaa acgcttgaga tacaactcaa acgagatgcg caggtacata tcctggtcga
      781 gttcgttgtg atgggtcaca aacggacgtg ccgccgcacc accgtaaaga ggctgaagga
      841 tcggcgtttc tacttcaagg aagttatgat catcgaggaa ggtacgcagg gacttaatga
      901 gcttggcacg tttacggaac gtatcacgaa catccggatt caccgccaaa tctgcataac
      961 gctgacgggc acgtagttct acatcatcaa gtgacgcata acgaaccacg gtgccatctt
     1021 cctgagtcac gtctttgtca gccgggagag gagagatgga tttggcgagc aggttgaagt
     1081 cgaggacttg aagcgaagcc tcgcccgcct tggtgcgcat catgatgccg gtcgcttcaa
     1141 cgaaatcgcc gaggtcgaac atcttattga agaaggcgag gcgatcctcc cccacttcgt
     1201 tcacgcgcag aaagagctgg atcttnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1261 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1321 nnnnnaaaaa agccccgcat cccaaaaata ggacgcgggg caacgaggcg cgtcctattt
     1381 aggcgacctt gaggatcttt acgttgtagg taccgccggg ggtttccact ttcacggtgt
     1441 cgcccacttt gtgacccaaa atagccttcc caatgggaga ctcatttgag atcttgcctt
     1501 cacgtgggtt ggcttctgct gcaccaacaa tggtgaatgt ttcggcttcg taattgcctt
     1561 ctttgatggt tacttttgag ccgacttgaa tggcatcgtg ctttttgcct ttgccattat
     1621 cttcaatgat ctgagctgtg gcaagcaaaa ggtctagctc ttgaatgcgt ccttcgacaa
     1681 aagcctgttc attcttagcg gcttcgtatt cagcattttc gatcagctcg ccaccttcca
     1741 tggcttcgtg caggcgctcg gcaacttcct ggcgtttaac ggtgcgcagg tggtcaagct
     1801 cctcttgaag cttgtcataa ccctctttgg taaggaaact ggttggcatt ggtttgtcct
     1861 gctaaatgaa ttttgcatcc cctaccgaga tgcaatgatt aaaaatttcc ggtggtactt
     1921 cccggaaaac gcgcatacta tatcatgatt tatagcgggt ttcaagcccc caaaagtatt
     1981 accttttttg tctaattcgt cgtccaaaaa ggcataccct taaattcgga gcagtactgg
     2041 ttgatttgag cccgcatcgc ccgttgactt ttgaatagtg acgagatctg ggatttgtat
     2101 gccaaaatcg cctctttcca agacgcaagc ccgtctctgc tcacccgttc gaccgttttc
     2161 ttcatcccaa ccgtgttcgg agccaaatga tcgggtgtgt tgaaataata tggaatatcg
     2221 gtcacataat gaagcggacg cccaagcaac tctgctgcac ggcgcacggt gacatggtcc
     2281 acgtgtttgc caacgccaaa ttggcagatc agtttatcgt ctgatttgag gcgagcggaa
     2341 atggcagaag caatttcggc ggggagattt gcctcgctgg gatgcgggtc cacaaagaca
     2401 tcggtataga gccaattccc ttccacatcg cggcggtaga tacaatctaa aaagtcgaag
     2461 tgccgcacct tggcgccaag aatagtagcg gcggctttat cctctgcacg gcgcatgcga
     2521 atcacctgac gtgccgtagg cataccccac tgcatatgca gaatgcgtgc aaacatagaa
     2581 agttctttgc tgggcgggta tccacacaga aacgtccata tctcaacttt gttgcctgcc
     2641 tgagtttggt cgtagatcca gccaccagcg gagagggcgg catcatcaag gtgaggggaa
     2701 agataaatcc agcgcatggt gcggtatttt taccacaaaa cagcaatgga acttgcccac
     2761 cccttccttt acacttgtgc gaaatgaggt tttatgcagg aacgacgtaa agaagagcga
     2821 aaaaatttag ttgcttatac acaggtgtac gacctatacg gtgggaattt gcttggctac
     2881 ctgggtgatt taactatttc aggggcaatg atcatcagtg aaaaagccat gaagcccgat
     2941 accgaaatca cactcgccat tgaactgcct gagatgcctg ggatcaaggc aattcgtatg
     3001 agcctattaa cccgggtggc atggtgcgaa caagacctaa gcccgcaata tttcaatatc
     3061 ggttttgagt tcaaagaaat taccccagat caaaaaggtt tgatcgaagc cattatcaaa
     3121 aactatgaat tccggcggga cgtccccaaa tatccaactc gtggagcacc accaaagcag
     3181 tagaaaacaa aaggtgacag tctgaaaaga ctgtcacctt tttatgttaa actttcgcca
     3241 tgtttcctgc taaaacttta ttttttacag ccccccggca agtggaagtt cgagagtcta
     3301 acctccctct cttgaaagag gatgaagttt tagtcgaaac catttgctct gccattagcg
     3361 cagggacaga gatgctggtc tatcgtgatc aatttccaca gttgaaagac tcgcatgatg
     3421 cggttagcag cgagttgaag tacccgctgg cgtatggtta tgcttgtgtg gggaaagtgc
     3481 gcgagattgg caaggtggtt ggcaaagaat ggcaagatcg cctggtattt tcgtttcagc
     3541 cacacacgtc tcattttgta agtaaacctg aaaacctgtt ccccattcca aattcgctta
     3601 agcctgaaaa tgcctgcttc cttccaaaca tggaaacagc cgtcaacctt gtgcaagatg
     3661 gcgctccaat cttaggcgaa cgcgtgctgg tgttgggtca agggatcgtt ggtctgctaa
     3721 cggcttctct gcttcatgag tttccgcttg agaatttgac cgttgtagat gcaattgaat
     3781 tacgacggaa agcaatgaaa gaaatcgaag gtcaaatgtc aaaggtcaat aaactttcac
     3841 ccagcgacct tcaatcttcc agttttgacc tcgtctttga actcactggc tctccttctg
     3901 ctttgaatac tgccattgaa cacacctcat tcagcggacg gatcgtaatt ggttcatggt
     3961 atgggcaaaa acgagcagaa atcgaccttg gcggttcatt tcatcgctca cgcatccaac
     4021 tgatttcttc acaagttagc agcatttcgc ccacgctaag cggccgctgg gacaagtctc
     4081 gtcgcttcga ggtggcatgg caagccttgg agcgcatcca accacagaag tggattac
//
LOCUS       DAOQJE010000208         4134 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_13146_length_4134_cov_6.665849, whole genome shotgun sequence.
ACCESSION   DAOQJE010000208 DAOQJE010000000
VERSION     DAOQJE010000208.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 4134)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4134)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4134
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13146_length_4134_cov_6.665849"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..231
                     /locus_tag="PLT08_06885"
     CDS             <1..231
                     /locus_tag="PLT08_06885"
                     /inference="COORDINATES: protein motif:HMM:NF013592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family aminopeptidase"
                     /protein_id="HNE04224.1"
                     /translation="QGFRPYTNATYFQGAHFLEKVRERIGDEAFFAFVQDYLNQGRGK
                     IVTANDFFRILRSHSGTDISDLINQYFRSVYQ"
     gene            231..1823
                     /locus_tag="PLT08_06890"
     CDS             231..1823
                     /locus_tag="PLT08_06890"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: protein motif:HMM:NF013592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family metallopeptidase"
                     /protein_id="HNE04225.1"
                     /translation="MKRYAFSISYYLLLSALLAACAAPPSTPTPQPFGQFILPTLDPS
                     KATVTPFQPAGQTNTSVPTYTASPLPTSTATITPPPPTVTFTSAPAATVVVSTTQAPP
                     PPVTTSTRPNYILYSTLDFKNRTIATDETIRYYNTTGTTLSDLVLSVQPNTYTNAFTL
                     NAIAQDGTALTSYTLSGQRLSVNLNQPLASGSATTITLSFNIKIPQKASGEVFGYDFN
                     QINLVDWYPFIVPYEGGWVLHDAMPWGEHLVYDAADIELNIKTDSGVTLAVGASPEAN
                     GEWTRYRMYGARQLALSASNEFIVNETTWGNVTIRSYYFNGYAKGGDDMLIYASEALQ
                     TYSTEFAPYPHQSLAIVQSDMDDGQEYDGLVFLATDFYSQYTGSAKSNLTTIGVHEIA
                     HQWWYSLVGSDHAFEPWLDEAMATYSERIFYENNYPANISWWWQFRVNYFKPTGYVDA
                     TIYDYGSFRAYTNAVYFQGALFLDDLRELMGYGNYSKFIKTYATRYANGHATTADFFA
                     LAREIVNINYDNLIAKYFSRSY"
     gene            1965..2663
                     /locus_tag="PLT08_06895"
     CDS             1965..2663
                     /locus_tag="PLT08_06895"
                     /inference="COORDINATES: protein motif:HMM:NF013989.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HNE04226.1"
                     /translation="MNRPYTFINVAMTADGKIDTFERKGAAISSKQDKQRVDELRAMA
                     DAILVGGKTLLEEQPKLTVKSEALREERIRQGRSPNPIKVGVATVADIQKDSDFLKAG
                     DARVVIFTTSQTSISQIENLRALGVNVFVDDAPRVDLHKMMITLKKLGVDHLMVEGGG
                     TMNFELMRLGLVDEVLIYVAPMIFGGANSPTLADGLGVTRDHALPMNLTGVEPWDDGG
                     VILKYKFFDQRREP"
     gene            2660..3418
                     /gene="ribA"
                     /locus_tag="PLT08_06900"
     CDS             2660..3418
                     /gene="ribA"
                     /locus_tag="PLT08_06900"
                     /EC_number="3.5.4.25"
                     /inference="COORDINATES: protein motif:HMM:NF001591.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003935 - GTP cyclohydrolase II activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase II"
                     /protein_id="HNE04227.1"
                     /translation="MTTLMHEKIQVLLEEDCCHECDGYGEDHVCVHIESMAELPTRFG
                     DFHIVAFSNNRDGKEHVAIVKGDVIGEEDVPVRLHSECLTGDAIGSLRCDCRDQLEAA
                     LKKIGEMERGMVLYLRQEGRGIGLTNKVRAYALQDTGLDTVEANLALGFRDDERDYAV
                     AAHMIHSLKVKSIKLMTNNPKKIAQLEQHGVKINERMPHILPMNEHNIFYLKTKAEKS
                     GHMIDFHGKEHLPEQSDRAIVEGMSEDQISALYE"
     gene            3418..4086
                     /locus_tag="PLT08_06905"
     CDS             3418..4086
                     /locus_tag="PLT08_06905"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HNE04228.1"
                     /translation="MNRNIIITGATGALGSLTAKTFASMGHNLALLDKDQAKLDALVR
                     DLNLPTERLFAQTVDLLNEQSLQTSAEAVLSKFGSVHALFHLIGGWVGGKTFTDTSAE
                     DLEFMINQHIRTTFNLFKVFEGPLAKNGWGRVIAVSASTVPNPPGKSGVYTATKSAQE
                     NMVLSLAAELKEAHVTANIIHVRAIDVDNKGTGTTPAEIVSAMQYLFSDAADKVNGTR
                     LPLY"
BASE COUNT         1078 a         1160 c          949 g          947 t
ORIGIN      
        1 cagggcttcc gcccatatac caatgccact tacttccaag gcgcacactt cctcgagaag
       61 gtccgcgagc ggattggtga tgaagccttc ttcgcctttg tgcaagatta tctcaaccaa
      121 ggtcgtggca agatcgtcac agccaatgat ttcttccgca tcttgcgttc acactctggc
      181 accgatattt ctgacctaat caaccaatac ttccgcagcg tctaccaata atgaaacgtt
      241 acgcattcag catttcatac taccttttac tttctgcctt gcttgccgct tgtgctgcgc
      301 cgccatcaac acccacgccg cagccctttg gtcaattcat cttgccaaca cttgacccca
      361 gcaaagccac cgtcacaccc ttccaacccg ccgggcagac gaacacatcc gtaccgacct
      421 acacagcatc accactccca acgtcaacag cgaccatcac accaccgcct cccacagtga
      481 cgttcacttc ggctcctgcc gcaacggtag ttgtttcaac cacccaagcc ccacctccgc
      541 cggtaaccac atccacccgc cccaactaca tcttatattc cacgctggat ttcaagaatc
      601 gaaccattgc aaccgacgaa accattcgct actacaacac caccggaaca acgctctcag
      661 acttggttct ctccgttcag ccaaatacgt acaccaacgc tttcactctc aacgccattg
      721 cacaagatgg cacagcgctc acctcctaca ccctcagcgg acaacgcctg agcgtaaatt
      781 taaatcaacc cttggcatcg ggttctgcca caaccatcac cctgagcttc aatatcaaga
      841 tcccgcaaaa agcctcgggc gaagtcttcg ggtacgactt caaccagatc aaccttgtgg
      901 attggtatcc cttcatcgtg ccatacgaag gcggctgggt tttacacgat gccatgccat
      961 ggggcgagca tctcgtctat gatgccgcgg atattgagtt gaacatcaaa actgactccg
     1021 gcgtgaccct cgctgtgggc gcttcgcccg aagccaacgg cgaatggact cgctaccgca
     1081 tgtacggcgc gcgccaattg gctttatctg ccagcaatga attcattgtc aatgaaacca
     1141 cttggggcaa tgtcaccatc cgctcgtatt atttcaacgg ctatgccaaa ggcggcgatg
     1201 acatgctcat ctatgccagc gaagcgttgc aaacctattc cacagaattc gcaccctatc
     1261 cgcatcaatc tctcgccatt gtgcaatcag atatggacga tggacaggaa tacgatggct
     1321 tggtcttcct cgctaccgat ttctacagcc aatatacagg ctctgccaaa agcaacctga
     1381 cgaccattgg cgtgcatgag atcgcccatc agtggtggta cagcttggta ggcagtgacc
     1441 atgccttcga gccgtggctg gatgaagcca tggcaaccta tagtgagcgc atcttctacg
     1501 aaaataacta cccagccaac atcagttggt ggtggcagtt ccgggtcaac tacttcaaac
     1561 cgactggcta tgtagatgcg accatttacg attatggctc tttccgtgct tataccaacg
     1621 ccgtgtactt tcagggcgca ctcttccttg atgacttgcg tgaattaatg ggctacggca
     1681 attactcaaa gttcatcaaa acgtatgcta cgcgctatgc caatggacac gccaccacag
     1741 cagacttttt cgccttggcg cgtgagattg tcaacatcaa ttacgataat ttgatcgcga
     1801 aatatttttc aaggtcatat taaccctaac tttggcaagc cacattctct aactttcgag
     1861 ggttggataa acctgcccat caatcctata atcaagccat cctttatgga tggcttgatg
     1921 ttttgtaaga agagctgggt acgacctgtc agtgaacaaa tcttatgaac agaccttata
     1981 cctttatcaa cgtcgcgatg acggcagatg gcaagatcga cacctttgag cgcaaagggg
     2041 ctgctatatc gtctaaacaa gataaacaac gtgtagacga attgcgcgcc atggcagatg
     2101 cgattctcgt cggcgggaaa acgcttttgg aagaacagcc caagctcaca gttaagagtg
     2161 aagcgctgcg tgaggagaga ataaggcagg ggcgttcgcc caaccccata aaggtcggcg
     2221 tggctaccgt cgcagacatc caaaaagact cagactttct taaggcgggc gacgcgcggg
     2281 tcgtaatatt tacaacatct caaacatcta tatcgcagat tgagaatttg cgcgcgctgg
     2341 gcgtgaatgt gtttgtagat gatgcccccc gtgttgacct gcacaagatg atgatcacgc
     2401 tgaaaaaatt aggcgtggat catttgatgg tagaaggcgg tgggacgatg aacttcgaac
     2461 ttatgcggct tggtcttgtt gacgaagtgc tgatctatgt agcccccatg atctttggcg
     2521 gcgcgaattc tcctacactt gccgatggtc ttggtgttac tcgtgaccat gccttgccca
     2581 tgaacctgac aggcgtggaa ccctgggacg acggcggtgt gattttgaaa tacaagtttt
     2641 ttgaccaacg gagagaacca tgacaaccct aatgcatgaa aaaattcaag ttttactaga
     2701 agaagattgc tgccacgaat gtgatggcta cggcgaagat cacgtttgtg tacatattga
     2761 atcgatggca gaactgccca cccgctttgg cgattttcac attgtggcat tttcaaataa
     2821 ccgtgatggc aaggaacatg ttgccattgt caaaggcgat gtgatcggcg aagaagatgt
     2881 gcccgtgcgc ttgcactcag aatgccttac cggcgatgcg attggctcgc tgcgctgtga
     2941 ctgccgcgac cagctcgaag cggctcttaa gaagatcggc gaaatggaac gcgggatggt
     3001 tttgtatctg cgtcaggaag ggcgtggcat tgggctgacc aacaaagtgc gcgcttatgc
     3061 tttgcaagat accggtctcg ataccgttga agccaatttg gcactcggtt tccgtgacga
     3121 tgaacgcgat tacgctgttg cagctcacat gatccactcc ctcaaggtca agtccatcaa
     3181 gctaatgacc aacaacccca agaagatcgc ccagctcgaa cagcatggtg taaaaatcaa
     3241 cgaacgcatg ccacacattc tgccgatgaa cgagcacaac atcttctacc tcaaaaccaa
     3301 agcagagaaa tctggtcaca tgatcgactt ccacggcaaa gaacacctgc ccgaacaaag
     3361 cgaccgcgcc attgtagaag gcatgagcga agaccagatc agcgcgttgt atgaataatg
     3421 aatagaaaca ttatcatcac tggtgcaacc ggcgctcttg gaagccttac cgccaagacc
     3481 tttgcaagca tgggtcacaa cctggcattg ctcgataaag accaagccaa attggatgcc
     3541 ctcgtccgcg acttgaacct gcccaccgag cgactcttcg cccaaaccgt ggacttgctc
     3601 aatgagcaat cgcttcaaac ctctgccgaa gctgtacttt ccaaatttgg cagtgtccac
     3661 gccttgttcc acctcattgg cggatgggtc ggcggcaaga ccttcactga tacctctgcc
     3721 gaagatctag agttcatgat caaccagcac attcgcacca ccttcaactt gttcaaagtg
     3781 tttgaaggtc cgctcgcaaa aaatggctgg ggacgtgtga tcgccgtatc cgcttctact
     3841 gtgcccaacc cgcccgggaa atctggcgtg tacacggcaa caaaatcggc gcaagagaat
     3901 atggtgctct ctctcgccgc cgaattgaaa gaagcacatg tgacggcaaa tatcatccac
     3961 gtacgcgcca tcgacgtgga caacaaaggc accggcacca cgcccgcgga gatcgtctct
     4021 gccatgcaat atctcttctc cgatgcagcc gataaggtca acggaacgag attgccgttg
     4081 tattaggaag taacaacaaa aaagtgaccg ccgaacggcg gtcacttttt ataa
//
LOCUS       DAOQJE010000209         4125 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_13190_length_4125_cov_6.242998, whole genome shotgun sequence.
ACCESSION   DAOQJE010000209 DAOQJE010000000
VERSION     DAOQJE010000209.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 4125)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4125)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4125
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13190_length_4125_cov_6.242998"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..191)
                     /locus_tag="PLT08_06910"
     CDS             complement(<1..191)
                     /locus_tag="PLT08_06910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE04229.1"
                     /translation="MLRSRTFLYFLIGISLLIALVFGGYYLYNTYRVRTVVIATNEEG
                     SQSYIMMDALAKAVQQRDSR"
     gene            complement(209..1042)
                     /locus_tag="PLT08_06915"
     CDS             complement(209..1042)
                     /locus_tag="PLT08_06915"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNE04230.1"
                     /translation="MSIVNRLLSIMHLQSLTPRPFLDSDLNEFPFTVPVIRSLPEIKF
                     TSPVTFFVGENGSGKSTVLEALACAVESITVGTESVKTDKTLAPVRKLSKYFKLAWTK
                     RTRKGFFMRAEDFFGYARSMRETREELEEEYNNVERDYEGRSKYAADLARSAYAGQLA
                     AMKSRYGRDLDNFSHGEAFLTLFQARFVPGGLYLLDEPEAALSPSRQLSFLSALRQMV
                     EEEAQFIIATHSPIILAYPGAQIFSFQNGAIKQVQYEELEHVQLTKDFLNNPQSYLRH
                     L"
     gene            complement(1073..2326)
                     /locus_tag="PLT08_06920"
     CDS             complement(1073..2326)
                     /locus_tag="PLT08_06920"
                     /inference="COORDINATES: protein motif:HMM:NF012766.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M24 family metallopeptidase"
                     /protein_id="HNE04231.1"
                     /translation="MKSDLDAIMQSRNLDAFVVFGNAENNPPMYYLTGGGHVSHATVI
                     KKRGEDAVYFYNDMERDEAAKSGLKLTPYSKYDYNELLQKANGDLLLTSALRYQQMFE
                     DVGVTKGRVGVYGHYDVGAVFGWLNHLQKLMPAIEFVGEARDDSIFLRAMETKDENEV
                     ARIRKMGKVTTAVVEKVKEYLTSCDVREDEVLLKEDGTPLSVGDVHSKIRLWVSEHGA
                     ELPSGFIFAIGRDAGVPHSAGNPADLMRLGQTIVFDIYPAEAGGGYFYDFTRTWSLGY
                     TTPEAQTLFDQVKEIYDTLNDNFDLNVAFKDYQRMTCEFFEGKGHQTPLNTKAPIEGY
                     VHSLGHGVGLNIHERPFSGLTAGDDQRLRPGVIITSEPGLYYPEKGMGFRIEDTLWVR
                     PDGVMEKLADYPYDFVLPMKKWKKK"
     gene            complement(2323..3690)
                     /gene="selA"
                     /locus_tag="PLT08_06925"
     CDS             complement(2323..3690)
                     /gene="selA"
                     /locus_tag="PLT08_06925"
                     /EC_number="2.9.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004125 - L-seryl-tRNASec selenium
                     transferase activity [Evidence IEA];
                     GO_process: GO:0016260 - selenocysteine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-seryl-tRNA(Sec) selenium transferase"
                     /protein_id="HNE04232.1"
                     /translation="MTTLRNLPSVEQLLQTETTARLIARFGRPLTLDALRGTLDDIRA
                     RFKINPEAGLPSIDSILNQAESTLTAWTAATLHPVINATGVILHTNLGRAPLSNATLH
                     AMDLVSRGYSNLEFDLETGKRGSRLIHAEGLLKKLTGAEAALVVNNCASAVLLTLSAL
                     ANKKRVVIARSQLVEIGGGFRVPDVMKQSGAKLVEIGTTNKVRVADYTSALEESTALV
                     MRAHRSNFKIVGFTEEPDFKDIVDAAHKAGIPVVDDLGSGALYDTAKYGFSHEPTVQE
                     SMQAGADVILFSGDKLLGGPQAGIIIGKKELLDRIKKHPLARAVRADKSCLAGISATL
                     THYLKDEAEREIPIVRMMSLTLKQVKGRAEAWREELGQGNVVEGESTVGGGSLPDESI
                     PTFLLELQVKSAEKFLRALRKNNPPIIARTENDKVLLDPRTVFPEQEGALLVGLKNLL
                     KKENK"
     gene            complement(3674..>4125)
                     /locus_tag="PLT08_06930"
     CDS             complement(3674..>4125)
                     /locus_tag="PLT08_06930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE04233.1"
                     /translation="LIQKSDASIIPTMDEKLHFKPFGLSALALALLGWGGLFLLITLT
                     LPFVWSRWGFFVLLLMALTGTALPVVFYLHQRFPTEPPADANVIIRQAMWVGVYGATL
                     AWLQLGRLVTLYIILGLAGGLIAAEYFIRIREKANRRPPMIHDDYTS"
BASE COUNT         1083 a         1074 c          975 g          993 t
ORIGIN      
        1 cgagaatcac gctgttgaac cgccttcgcc aaagcatcca tcatgatgta actctggctg
       61 ccttcttcat ttgtcgcaat aaccaccgta cgaacacggt acgtgttgta caagtaatat
      121 ccaccaaaca ccaatgcgat caataaggaa atgccgatca agaaataaag aaaagtgcgc
      181 gagcgaagca ttgagatcat tcctgtatct ataaatgccg caagtaagat tgcgggttat
      241 tcaaaaaatc cttggtaagt tggacgtgtt caagctcctc gtattgtact tgctttatag
      301 ccccattttg aaagctgaat atttgcgccc ccggatacgc caaaatgatc ggcgaatgag
      361 tagcgatgat aaactgcgcc tcttcttcga ccatttgcct gagtgccgaa aggaagctga
      421 gctgtcggga cggcgaaagc gccgcctcgg gttcatccaa cagatataag ccgccgggga
      481 caaaacgcgc ctgaaacaaa gtgaggaatg cctcgccgtg cgagaagttg tccaagtctc
      541 taccgtaacg gctcttcatc gccgcaagtt gaccggcata cgccgaacgt gccaagtcag
      601 cagcatattt cgagcgtcct tcataatcgc gctctacatt gttgtattct tcttccagtt
      661 cttcacgcgt ctctcgcatg gaccgggcat atccgaaaaa atcttcggcg cgcataaaaa
      721 agcccttgcg cgtacgtttc gtccatgcaa gtttgaaata ctttgatagc ttacgaacag
      781 gcgcaagggt tttatcggtc ttgacgcttt ccgtccccac cgtaatggac tctaccgcac
      841 aagccaatgc ctctaacacc gtcgatttgc ccgagccgtt ctcccccaca aaaaaggtta
      901 ccggtgaggt gaacttgatc tctggcaaag aacgaatcac cggcaccgtg aacggaaatt
      961 cattcaggtc actatcgaga aatggacgag gtgtaagcga ttgaaggtgc atgatggaca
     1021 atagacgatt gacgatggac attctttcat ggtccatcgt caatcgtcga cattatttct
     1081 ttttccactt cttcattggc aaaacaaaat catacgggta atctgccagc ttttccatga
     1141 cgccatcagg gcgaacccaa agcgtatctt cgatacggaa gcccatccct ttttcggggt
     1201 agtacaagcc cggttcggag gtaatgatca ctccgggtct gaggcgttga tcgtcccccg
     1261 ccgtgagccc actgaatggg cgttcgtgga tgttcaaccc aacaccatgc ccaaggctgt
     1321 ggacatatcc ctcaattggc gctttggtat tgagcggagt ttgatgccct ttgccttcaa
     1381 agaattcgca ggtcatgcgt tgataatcct tgaatgccac atttaagtca aaattatcgt
     1441 tgagcgtatc gtagatttct ttgacttggt caaacaatgt ttgcgcttca ggcgtagtat
     1501 agccaaggct ccatgtacgg gtgaaatcat agaaatagcc accacctgct tctgccggat
     1561 aaatatcaaa aacaatcgtc tgacccaaac gcatcaggtc agcggggttg cccgcagagt
     1621 gcggcacacc ggcgtcgcga ccaatggcaa agataaaacc cgaaggcaac tctgcgccat
     1681 gttcagaaac ccaaagccgt atcttcgagt ggacgtctcc tacgctaagc ggagtgccat
     1741 cttccttgag taacacctca tcctcgcgta catcgcaaga ggtaaggtat tcctttacct
     1801 tctctacgac tgcagtcgtt accttaccca ttttgcggat acgcgcgact tcattctcat
     1861 ccttggtttc catggcgcgc aaaaagatag agtcatcacg tgcttcaccg acaaactcaa
     1921 tcgcgggcat caacttctga agatggttca gccacccaaa caccgcacca acatcataat
     1981 gaccatatac accaacacgt ccctttgtca ccccaacatc ttcaaacatc tgttgatacc
     2041 gcaaagcact tgtcaacaaa agatcgccat tggctttctg taacaattcg ttatagtcat
     2101 atttgctata gggagttaac ttcaatccgc tcttggcggc ttcatcgcgc tccatatcat
     2161 tgtaaaaata aaccgcatct tcgccgcgtt tcttgatcac cgtagcatgg ctaacatgcc
     2221 caccaccggt cagataatac atgggcgggt tattctcagc attaccaaat acaacgaatg
     2281 catccaagtt gcgagattgc ataatggcat cgaggtctga tttcatttat tttccttttt
     2341 caacaaattt ttcaacccaa ctaataacgc accttcttgt tcaggaaaaa cagtacgagg
     2401 gtcgagcagc actttatcat tctctgttcg agcaataata ggcggattat tctttcgtaa
     2461 ggcgcgtaaa aatttttcgg cagatttcac ttgcaattca agcaaaaagg tcgggatgga
     2521 ttcatccgga agcgacccgc cccccaccgt agactcacct tccactacat tgccttgacc
     2581 taactcctct cgccacgcct cagcccgacc tttgacctgc ttcagcgtta gagacatcat
     2641 cctcacaatg ggaatttctc gctccgcctc gtctttgagg taatgtgtga gcgtagcaga
     2701 gatgcctgcc aaacaagatt tatctgcgcg gacggctctc gccagcggat gtttcttaat
     2761 tctatcgagc agctcctttt tgccgatgat gatgcccgcc tgtggaccgc caagcagttt
     2821 atcgccggaa aagagaatca catccgcccc agcctgcata gactcttgaa cggtcggttc
     2881 gtgagagaag ccatatttgg cggtgtcgta caaagcgccc gagccgaggt catctacgac
     2941 gggaatgcca gctttatggg cagcatcgac tatgtctttg aaatcgggtt cttcagtaaa
     3001 gcccacgatc ttgaaattcg agcgatgcgc acgcatgacc aaagcggttg attcttccaa
     3061 cgcggaggtg taatctgcca cgcggacttt gttggtggtg ccgatctcca ccaactttgc
     3121 acccgattgc ttcatcacat cgggcacgcg gaagccgcca ccgatctcca ctagttgcga
     3181 gcgtgcgatg accacgcgct ttttatttgc caaggctgaa agcgtaagca acactgccga
     3241 agcacagtta ttaacgacca atgccgcctc tgcacctgtg agttttttca acaacccttc
     3301 agcatggata agacgtgacc cgcgtttgcc tgtttcaagg tcgaactcaa ggttggaata
     3361 gccacgtgag accaaatcca tagcatgaag ggtggcgttg ctcaatgggg cacgccctag
     3421 attggtatgc agaatcactc cggtggcgtt aatgaccgga tggagcgtgg cagccgtcca
     3481 tgcagtgaga gtggattcgg cttgattgag gatcgagtct atagagggca accccgcttc
     3541 gggattaatt ttgaagcgcg cgcggatgtc atccaacgtg ccacgtaacg catctaaggt
     3601 caacggacga ccaaagcggg caatgagtcg cgcggtcgtt tcggtttgaa gaagttgttc
     3661 gacggaggga agattacgaa gtgtagtcat cgtggatcat gggcggacgg cgattggctt
     3721 tctcacggat gcgaatgaaa tactcggcgg caatcagccc acctgctaga ccgagaatga
     3781 tgtataaggt cacgaggcga cccaattgca gccaggcaag agtggcgcca tagacaccca
     3841 cccacatggc ttggcgaatg atgacattcg catcggcagg cggctcggtt ggaaagcgtt
     3901 gatgtaaata aaaaacgact ggaagtgccg tgccagtgag cgccataaga aggaggacaa
     3961 aaaatcccca acgggaccaa acgaaaggca aagtaagggt gataagaaga aacaagccac
     4021 cccagccaag aagagcaagt gctaaagcgg ataaaccaaa tggtttgaaa tgcagttttt
     4081 cgtccattgt tggaattata ctcgcatcag atttttgaat gagcg
//
LOCUS       DAOQJE010000210         4122 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_13207_length_4122_cov_6.375461, whole genome shotgun sequence.
ACCESSION   DAOQJE010000210 DAOQJE010000000
VERSION     DAOQJE010000210.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 4122)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4122)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4122
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13207_length_4122_cov_6.375461"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..615
                     /locus_tag="PLT08_06935"
     CDS             <1..615
                     /locus_tag="PLT08_06935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE04234.1"
                     /translation="MDAHPDDAQNFISLYNEIYALVKAEAPDTQIFVTFQWDDLNNMF
                     AQPEEGDAQRLSPKWEQIEAFEPNLDVWAISSYPYFVFPAGADIPSDYYAPLLSRTSK
                     PVALAEGGFSTVAFNELNNTPEDQVAYLNAIHEQLGPRMAFWVNTLLSDFNIDSYAKE
                     MEKQGRNPDDAQMLGNFAYIGLREFDGTPKPALKVWDGFRNSTP"
     gene            complement(612..1046)
                     /locus_tag="PLT08_06940"
     CDS             complement(612..1046)
                     /locus_tag="PLT08_06940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE04235.1"
                     /translation="MNIDDLRVRLEEEACSRANYSIGERDSDVFCLENQNGIWRIFYT
                     ERGIDQPPIFESPNESEACEYFFTFMTTKIRHDHIVGYFISREKAEALVETLAQHNIQ
                     TYRNDIPYHGWHDPRFRVFVIGKDVFKAREILGELPVRDIRD"
     gene            complement(1043..2209)
                     /gene="mltG"
                     /locus_tag="PLT08_06945"
     CDS             complement(1043..2209)
                     /gene="mltG"
                     /locus_tag="PLT08_06945"
                     /inference="COORDINATES: protein motif:HMM:TIGR00247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008932 - lytic endotransglycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HNE04236.1"
                     /translation="MNDTQPQAPVRRRKPRNSMRRYQLYFALGSIVLVAFICIFVFIY
                     YVPAQATLLYGPPAGHLSISDRIEYSTRLLSHGTELTTPLNVNGLEQSFRVEPGESVA
                     SIANRLEGLGIISSADAFFDYAVYTGIDLTIQSGDFNLSPAQSIVDIAQSLQKFNPSD
                     AVLSILPGWRIEEIAASLPTSGLSIAPEAFLAAAQTPPQVLAFAAPTTMEGFFFPDTY
                     TLPRTTTVDQLLDVVARNFTTKLTTDIQNGFAAQNLSVYQGVILASIVQREAVKIEEA
                     PVIASVYLNRISIGMKLDADPTVQYALGYQFDNGSWWKSPLALTDLEVISPYNTYLNN
                     SLPPTPISSPGLEALMAVAYPQSTPYYYFQAACDGSGYHVFVVTYEEQIANGCQ"
     gene            complement(2206..2625)
                     /gene="ruvX"
                     /locus_tag="PLT08_06950"
     CDS             complement(2206..2625)
                     /gene="ruvX"
                     /locus_tag="PLT08_06950"
                     /inference="COORDINATES: protein motif:HMM:NF015607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HNE04237.1"
                     /translation="MRILAVDHGEKRIGLALSDPTATIASPLKVIQHVSRLIDAAQVA
                     EIAMQNEVGLIVVGQSYDEEGDPNPAGRRAGRFADELRNQTNIPIEMWDESHSTQIAR
                     AARIELGVSRKKRAGHQDEFAAVVILQSYLEAKRNSE"
     gene            complement(2634..>4122)
                     /locus_tag="PLT08_06955"
     CDS             complement(2634..>4122)
                     /locus_tag="PLT08_06955"
                     /inference="COORDINATES: protein motif:HMM:NF016736.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="baseplate J/gp47 family protein"
                     /protein_id="HNE04238.1"
                     /translation="NLKTKIITLESHDDLISVRDKLSWAKTPRILLVWPKYEKVTLRI
                     LDLKVLQRHADSLGAQLGLVTRRMNVRHDAESLGIPVFRTTTAAQKESWGEPIPRVQR
                     TPKSPQKGLRQLRDEVYIQEPAWRTSLFGRVVAFTAGVMAVLVLAGLFVPRAVVTVHS
                     EAQAVSLVIPVNASPFFDSVSLSGEIPAQVISVTVRNEQTVQVSSLVSVPKTKATGVV
                     QFTNLGSDEIAIPAGTVVASNTLIRFATLNETRLPAGVDETVEVKVEALEAGAQGNVE
                     SGAISVIEGTLGLSAKITNLEPTTGGSDEEVLGATEEDRATLRDSVIAGLTSIAETQI
                     RSQIGASDLFISDTLTMKDIQNEEFTPPLGGEAPTLTYAVDAEFTAHYILEEDLKALA
                     ASSVMASIPQGFSPTGDMVFNPTETPFTDTTGVTRFALQATQSTLRDVNVMQVLDLIR
                     GRNVQQATLLVKDTLALQNEPQITMTPSWWKWLPLIPFNISVKVQ"
BASE COUNT         1074 a         1020 c         1002 g         1026 t
ORIGIN      
        1 atggatgctc atcctgatga tgcgcagaac ttcatcagcc tatacaacga aatctatgca
       61 ctcgtcaaag ctgaagcgcc tgatacgcaa atcttcgtca catttcaatg ggatgacttg
      121 aacaatatgt tcgcacaacc cgaagagggc gatgcgcaac gcctttcgcc aaagtgggaa
      181 cagatcgaag cttttgaacc gaatttggac gtatgggcga tctccagtta cccttacttc
      241 gtcttccccg ccggtgcaga tattccctct gattactatg ccccgcttct ctcgcggacc
      301 tcgaaaccag ttgcccttgc cgaaggcgga ttctccacgg ttgctttcaa cgaactaaac
      361 aacacccctg aagaccaggt tgcatacctg aatgctattc acgagcagtt aggtccacgc
      421 atggcgtttt gggtgaacac cttgttaagt gacttcaaca tagactctta tgccaaagaa
      481 atggaaaaac aagggcgcaa tccagacgat gcccaaatgc ttggaaactt cgcttatatt
      541 ggcttacgtg aatttgatgg cacacccaaa cctgcattga aagtttggga tggttttaga
      601 aattccacac cttaatctcg aatatcacga acaggcaatt cgcctaaaat ctcgcgggct
      661 ttgaacacat ctttgccaat cacaaatact cgaaagcgcg ggtcatgcca gccatggtag
      721 ggaatgtcat ttcggtaggt ttggatatta tgttgagcca atgtctctac caaggcttcg
      781 gctttttcac gagagataaa ataacccaca atatggtcat gccgaatttt ggtggtcatg
      841 aaggtgaaga aatattcaca agcttctgat tcgtttgggc tttcaaagat cggaggctgg
      901 tctataccgc gttcggtata aaaaatccgc cagattccat tttgattttc aaggcaaaag
      961 acatctgagt cgcgctcacc aatcgaataa ttggcgcggc tacaggcttc ttcttccaaa
     1021 cgcactctca ggtcgtcaat gttcattgac atccattcgc gatctgctct tcataggtta
     1081 ctacaaagac gtgataccca gagccatcgc acgccgcttg aaaataataa tacggcgtag
     1141 actgtgggta tgccaccgcc atcaacgcct caagaccggg gcttgagatg ggcgtgggcg
     1201 gcaagctatt gtttagatag gtgttatatg gcgagatgac ttccaggtcg gtcaatgcca
     1261 gcggactctt ccaccaagag ccgttatcaa attgatatcc caatgcatat tgaactgtag
     1321 gatctgcatc tagtttcata ccgatggaaa tgcgattgag atagacagaa gcaatgaccg
     1381 gcgcttcttc gatttttaca gcttctcgtt gaacaataga ggcaaggatc accccttgat
     1441 aaacgctgag attctgcgca gcaaaaccgt tttgaatatc ggttgtcaat ttagtggtga
     1501 agttacgcgc aaccacatcg agcaattggt ctactgtggt tgtgcgtgga agggtatatg
     1561 tatctgggaa gaaaaatcct tccatggtgg taggagcggc aaaggcaagc acttgaggcg
     1621 gagtctgtgc ggcggcgaga aaagcttcgg gtgcaatgga caagcccgag gtcgggagtg
     1681 aggcggcgat ctcttcgatc cgccaaccgg gcaagatcga gaggacggca tcgctgggat
     1741 tgaatttttg gagcgactga gcaatgtcta caatcgattg tgccgggctg aggttgaaat
     1801 caccagattg aatggtgagg tcaatgccgg tgtaaacagc gtaatcaaaa aacgcatcgg
     1861 cacttgagat aatgcccaac ccctcaagcc gattcgcaat ggacgccacg gactcacccg
     1921 gctctacccg gaatgattgt tcgagcccat tcacattcaa aggggttgtg agttcagtac
     1981 cgtgcgaaag taagcgagtt gaatattcga tacggtctga aatggaaaga tgcccggcag
     2041 gtggaccata caaaagtgtg gcttgcgcag ggacataata gataaacaca aagatacata
     2101 taaaagcaac caacacaatc gaccctaaag caaaatagag ttgatagcgg cgcatggagt
     2161 ttctaggttt tcgtcttcga actggggctt gtggttgagt atcgttcatt cggaattacg
     2221 tttcgcttct aaataagatt gtaagatgac cactgcggcg aactcatctt gatgcccggc
     2281 acgtttcttg cgtgaaacgc caagttcaat acgagcggca cgggcgatct gggtgctgtg
     2341 agattcatcc cacatttcga tgggaatatt ggtttgatta cgaagctcat cggcaaagcg
     2401 cccagctctg cgcccggctg ggttggggtc gccttcttca tcatacgact gcccgacaac
     2461 gattagacct acttcatttt gcatggcaat ctctgccacc tgagccgcat cgattaaacg
     2521 tgagacatgt tgaatcactt tcaaggggct ggcaatcgtt gcagttggat cgctgagagc
     2581 aagaccgatt cgtttttcac cgtggtcaac ggcaaggatt ctcatactat tgtctactgt
     2641 actttcactg aaatattgaa ggggatcaac ggcagccact tccaccaaga aggtgtcatg
     2701 gtgatttggg gttcgttttg caacgccaac gtatctttca ccaacaacgt cgcttgttgg
     2761 acgttccgtc cgcgaatgag gtctaaaacc tgcatcacat tcacatcgcg caacgtggat
     2821 tgcgtggctt gcaatgcaaa acgcgttaca cctgttgtgt cagtgaacgg cgtttcggtg
     2881 ggattaaaaa ccatatcccc cgtgggagaa aacccttgcg ggatggacgc catcacagat
     2941 gaagctgcca aggctttaag gtcttcttcc aaaatgtaat gcgcggtgaa ttctgcatcg
     3001 actgcatatg tgagagttgg tgcttcccca cccaaaggcg gcgtaaactc ttcgttttga
     3061 atatctttca tcgtaagcgt atcgctgata aacaaatcac tcgccccgat ttgcgaacgg
     3121 atctgtgttt cggcaataga agtcaagcca gcgattacag agtcacgtag cgttgcacgg
     3181 tcttcttcgg tagcgcccaa tacttcttca tcgcttccgc ccgtggtggg ttctagattc
     3241 gtaatcttgg cagaaagccc aagcgttcct tcaattacac taatcgcgcc agattcaaca
     3301 tttccttgcg cccctgcttc caacgcctcc acttttactt ccactgtttc atccacacca
     3361 gcaggcaagc gggtttcgtt cagtgtcgca aatcgaatca acgtattgga cgccactacc
     3421 gtcccagcag ggatggcaat ttcatcagag cctaaattcg taaactgcac cacaccggtc
     3481 gcttttgttt ttggcacaga aaccaagctc gatacttgga ccgtctgttc attgcgaacg
     3541 gtgacagaaa tgacttgggc gggaatctct ccacttaagg agacggaatc aaaaaatggg
     3601 ctcgcattga ccggaatgac aagcgagacg gcttgagctt cggaatgtac cgtcaccacg
     3661 gctcgcggga cgaataagcc tgccagcacc aaaacagcca tcacccccgc tgtaaacgcc
     3721 accaccctcc caaagagcga cgtgcgccaa gctggttcct gaatatagac ttcatcgcgc
     3781 aattggcgca aacccttttg tggcgacttg ggtgtacgct gtacacgtgg aatgggttca
     3841 ccccatgatt ctttttgtgc cgccgttgta gttctaaaaa cgggaatccc taaagactcg
     3901 gcatcgtgac gtacattcat acgacgtgtc accaacccca actgcgctcc cagtgaatcc
     3961 gcgtgacgtt gtaagacctt caaatccaat attcgcaagg tgaccttctc atacttgggc
     4021 cacaccagca aaatacgcgg cgtcttcgcc cacgataatt tatcgcggac agagatgagg
     4081 tcatcgtgag attcgagggt gatgatcttg gttttcaagt ta
//
LOCUS       DAOQJE010000211         4108 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_13268_length_4108_cov_6.398717, whole genome shotgun sequence.
ACCESSION   DAOQJE010000211 DAOQJE010000000
VERSION     DAOQJE010000211.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 4108)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4108)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4108
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13268_length_4108_cov_6.398717"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1846
                     /locus_tag="PLT08_06960"
     CDS             <1..1846
                     /locus_tag="PLT08_06960"
                     /inference="COORDINATES: protein motif:HMM:NF012869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNE04239.1"
                     /translation="FVYGMLNSPNMKNLIRSADFLRPYVWQVLASLLMLLVLTGLNLV
                     IPRIIQNVVDDGLLRGDTIYLVRSALLLFGLGVVSALLGLGNRYASEWVAAHVGYDLR
                     NRMYDHIQYLPFAYHDHAQSGQLISRCIEDVRAIEKFAGSSIPELMRFVLLTLGILGV
                     MLTTNSKLAIISLLPMLPLIVMTGWFGTKIGKLFFEVDQAVGEVSNRLQENVTGVQVV
                     RAFAREDYEIKRFESANMDVYHKWLHVINEWAKIMPTTNWLITIGTILILYFGGQMVI
                     AGELTVGAVVAFNAYLLMMAEPAQALTGLVNAGGEAAAGAQRVFEVLDTEPEIRSKKE
                     ALVLSELKGEVEFHNVSLKYQDENTRSLANINVKVERNKIVALIGPTGSGKTSLVNLI
                     PRFYDVTDGMVLVDGQDVRDVDLTSLRRQIGIVLQTSLLFSDTIRANIAYGRPDAKQE
                     EIVAAAKAAQAHEFIEGFTKGYDTIVGERGVTLSGGQRQRVAIARALLMNPRILILDD
                     SLSSVDTQTEKLIQAALDVLMEGRTTFVIAHRLSTVRRADMILVMDKGRIVEQGTHDE
                     LIKAGGLYKEIHDLQLVEREDDITAVQPIFGEKPLHENTIYSGKSNSG"
     gene            1851..3647
                     /locus_tag="PLT08_06965"
     CDS             1851..3647
                     /locus_tag="PLT08_06965"
                     /inference="COORDINATES: protein motif:HMM:NF012869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNE04240.1"
                     /translation="MTTKIIPPEFITKSEEQLDKGYDPKVASGLMKFVKPYYGQTIFA
                     LILMVIVTAASVSGPYFVKLAIDEGITAKDPVALRNITLTFLVVSVIQLGLNYWRVRI
                     MARVGQHVLYDVRTAMFAHLQKLSLSFYNRYSVGRVITRVINDVGTLREFITWAVLAI
                     VRNVLGIIGTLVAMIALNFQLSLLTFIVLPIMVWATIAFRKAARQNYRKVRAAVSWGN
                     SVLAENVNGVRVVQAFSRQQHNYNTFSGYVNRYYLETSLDAVRVASLFTPVIDLLGAL
                     ATALVIYVGGTAVLGESITAGVLIAFVLYIDRFFEPIRDLSRRFDTLQSTMAGGERIL
                     ELLHTPIEVRDEEHANEMGPITGAVRFDNVSFHYSDDPVLVLDSIQLDVKPGETVALV
                     GETGAGKTTIVKLLARFHDPTSGGVLIDGVDLRTVTQGSLRAQMGMVLQDPFLFNGTV
                     KENILFGRLDAKDEEVIATAKAVGAHEFIVNLKNGYDTSVEEGGLTLSVGQRQLISFA
                     RALLANPRILILDEATSSVDTQTEHVIQTALATLLQGRTSFVIAHRLSTITNADKIVV
                     IDGGKIVEQGKHAELIALQGRYYELYKTGFQE"
     gene            3651..>4108
                     /locus_tag="PLT08_06970"
     CDS             3651..>4108
                     /locus_tag="PLT08_06970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE04241.1"
                     /translation="MTEPTNEFLYKMSKHMRDGLADVQKKINDLYKIEELEEYVDIPD
                     SPIEHLKAAVMPLVIQVNDFMDYAETVTGRMELDLQDVDVHALLEGVLMMADQLVEMK
                     GGITLEEDIPDNLPTIEGDPTRLSQALLNYIHNAVKFTEKGTVTVRVAREA"
BASE COUNT         1011 a          801 c         1119 g         1177 t
ORIGIN      
        1 ttttgtttat ggtatgctta actccccgaa tatgaaaaat ctaatacgct ctgctgactt
       61 tcttcgacct tatgtctggc aggtgttggc tagcctgctt atgttgttgg ttctcaccgg
      121 attgaacctg gttattccgc gtattatcca aaatgtagta gatgatggac ttttgcgtgg
      181 cgatacgatt tatctggttc ggtctgcgct tctcttgttc ggacttggag ttgtttcagc
      241 gctgctaggg ttgggcaacc gttatgcttc tgaatgggtg gcagcgcatg ttggttacga
      301 tctgcgcaac cgcatgtatg accatattca atatttgccc tttgcctatc atgaccatgc
      361 ccaatctgga caattgattt cgcgttgtat tgaagatgtg cgtgcgattg aaaaatttgc
      421 agggtcttcc atccctgaat tgatgcgttt tgttttgctt acgctgggga ttttaggggt
      481 gatgcttacg acaaatagca aattggcgat aatctctctc ttgcccatgc ttccgctgat
      541 cgtaatgaca ggctggtttg gaacaaagat cgggaagctg ttttttgaag tcgatcaggc
      601 ggtaggagag gtctctaatc gcttgcagga aaatgtgacc ggggtacagg tcgtccgtgc
      661 ttttgcacga gaggattatg agatcaagcg ttttgagtct gccaacatgg atgtgtacca
      721 taaatggctg catgtgatta atgagtgggc aaaaattatg ccaacgacaa actggttgat
      781 cacaattgga acgattttga tcctgtactt cggcgggcag atggtgattg ctggcgaatt
      841 aaccgtcggt gcggtggtgg cattcaacgc gtatttgctt atgatggcag aaccagctca
      901 agcgttgacc ggtttggtta acgcaggcgg agaggcggcg gcgggtgctc aacgggtatt
      961 cgaagttttg gatacagaac cagagatcag gtccaagaaa gaggcgttgg ttttatccga
     1021 attgaaaggc gaggtggaat ttcataatgt ctcgttgaaa tatcaggatg agaatacgcg
     1081 ctcattggcg aatatcaacg tgaaagtgga acggaataaa atagtggcgt taattggtcc
     1141 gaccggctct ggcaagacat cacttgtaaa tttgattccg cgtttttatg atgtgactga
     1201 tggtatggtg ttggtggacg gacaggatgt gcgcgatgta gatttgactt ctcttcgcag
     1261 gcagattggg attgtgcttc aaacctcttt gttgttttca gacacgattc gcgcaaacat
     1321 tgcttacgga agaccagatg caaagcaaga agaaattgtt gcggcggcaa aagctgctca
     1381 ggcgcatgag tttattgaag gctttacgaa gggatatgac acgattgttg gcgaacgcgg
     1441 tgtgactctt tcaggtgggc agcgtcagcg tgtggcaatt gcgcgtgcgt tgttgatgaa
     1501 cccacgaatt ttgatcttgg atgattcgct ttcaagtgtg gatacacaaa ctgaaaaatt
     1561 gatccaagct gcattggatg tattgatgga gggtcgtaca acgttcgtga ttgcgcaccg
     1621 tctgagtacg gtgcggcggg cagatatgat tcttgtaatg gataaggggc gtatcgtgga
     1681 gcaaggcacc cacgatgagt taataaaagc gggcgggctt tacaaagaaa ttcacgactt
     1741 gcaattggtg gaacgcgaag atgatattac ggcagttcaa ccaatttttg gtgaaaagcc
     1801 attgcatgag aatactatct actctggaaa atcaaattcg ggataatgcc atgactacaa
     1861 aaataattcc tcctgaattt attaccaagt ctgaagagca actcgataaa ggctacgacc
     1921 ctaaagttgc cagcgggttg atgaagtttg tcaaaccgta ttatgggcag acgatttttg
     1981 ctttgatttt gatggtgatc gttaccgcgg cttcggtttc gggcccatat tttgtgaagc
     2041 tggctattga tgaaggtatt acagccaaag accctgttgc cttacgaaat atcacactca
     2101 cattccttgt tgtttcggtt atacaactag ggctcaacta ttggcgcgta cgcattatgg
     2161 ctcgtgtagg gcagcatgtt ttgtatgatg tgcgcacagc catgtttgct catttgcaga
     2221 agctttcatt gagtttctac aatcgctata gcgttgggcg tgttatcacg cgtgtgatca
     2281 atgacgtggg aactttgcgt gagtttatta cttgggcggt tctggcgatc gtgcgtaatg
     2341 tacttggcat cattggtaca ttggtggcaa tgatcgcttt gaattttcaa ctttcattat
     2401 taacctttat cgtcttgccg atcatggtat gggcaacgat tgcctttcgt aaagcggcgc
     2461 ggcaaaacta tcgcaaggta cgcgccgcgg tttcgtgggg caactcggtg ttggcagaaa
     2521 atgtcaacgg agtgcgagtg gtgcaagcat tttcgcgcca acagcataac tacaacacct
     2581 ttagtgggta tgtcaatcgc tattacctgg aaaccagtct cgatgcggtt cgcgtcgctt
     2641 cgcttttcac acccgtgatc gatttgcttg gggcattggc aactgcattg gttatttatg
     2701 tgggcggaac ggcggtactt ggcgaatcga ttacggctgg tgtgttgatt gcatttgttc
     2761 tatatattga ccgcttcttt gaacctattc gtgatttgag ccgccgcttt gatacgcttc
     2821 aatccacgat ggcaggtggg gaacgcatac ttgagttact ccatacgccg attgaggttc
     2881 gagatgaaga acatgccaac gaaatgggac ccattaccgg cgcggtgcga ttcgataacg
     2941 tctcgttcca ttattcggat gacccggttc tggttcttga ttcaattcaa ttggatgtaa
     3001 agcctggtga gacggttgcc cttgtggggg agaccggtgc tggcaagacg accatcgtta
     3061 agttgttggc acgcttccac gacccgacct cgggcggggt gctcattgac ggcgtggatt
     3121 tacggacggt gactcaaggc tcgcttcgcg ctcaaatggg tatggtgttg caagacccgt
     3181 tcctgttcaa tggtacggtg aaagaaaata ttttatttgg tcgcttggac gcgaaagatg
     3241 aagaggtaat tgctactgca aaggcggtgg gcgcccatga attcatcgtg aatttgaaaa
     3301 atgggtacga cacgtcagtt gaagaaggcg ggttgacctt gagtgtggga cagcggcaat
     3361 taatttcatt tgcgcgtgcc ttgttggcga atccgcgtat ccttattttg gatgaagcaa
     3421 cttcttctgt tgacacgcag accgagcatg ttattcaaac tgccttagca actctcttgc
     3481 aaggacggac ttcgttcgtg attgcacatc gtttgtccac aattacaaat gcagataaaa
     3541 ttgtggtgat cgatggtggg aaaatcgtcg aacaaggaaa gcatgcagaa ttaattgcct
     3601 tacaaggcag atattacgaa ctctataaga cgggttttca ggagtagatt atgacagaac
     3661 caacgaacga atttctttat aaaatgtcca agcatatgcg tgatgggctt gccgatgtac
     3721 aaaagaagat caatgacttg tacaagattg aagagttgga agagtacgtg gatattccag
     3781 attctcccat tgaacatctc aaggcggcag taatgcctct ggtgattcag gtcaatgatt
     3841 ttatggatta tgccgagacg gtcactgggc gcatggagtt ggacctacaa gatgtagacg
     3901 tgcatgcgtt gctcgaaggt gtgctgatga tggcagatca attagttgaa atgaaaggtg
     3961 gcattacgtt ggaagaagat attccagata atcttccaac gattgaaggt gaccctactc
     4021 ggctttcgca ggcgttattg aattacattc ataatgctgt gaagtttaca gagaaaggca
     4081 cagtgaccgt ccgcgtggca cgagaggc
//
LOCUS       DAOQJE010000212         4103 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_13290_length_4103_cov_3.113142, whole genome shotgun sequence.
ACCESSION   DAOQJE010000212 DAOQJE010000000
VERSION     DAOQJE010000212.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 4103)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4103)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4103
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13290_length_4103_cov_3.113142"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(191..1153)
                     /locus_tag="PLT08_06975"
     CDS             complement(191..1153)
                     /locus_tag="PLT08_06975"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNE04242.1"
                     /translation="MRDVFKNDREFNQWDKLVLDVKEVYKKHPLSVNVLEGAQLPPHT
                     ALFRRFYKTPLVPLSLRRFIHKLSLRTSLDTSWFEDFSNYWSSVLGGRPLLGVQDFYF
                     LKNLYRIRFQDNQIPDTNDVNIHLEAWQRPELLYQLLHLIYKETIVDYANLIKKMFEY
                     KPKLRNILEFGCGTAPVTASLYEFFEVRKEIKVYFSDIQTIAFHYAAYRFARYQNANP
                     VILKPENEFKLANLPPLDVITCITVFEHLNKPLDTVKDFHALLLPGGLFFFDYIKSDG
                     SGMDTNHGSQERLAVLKFVASHFDILEGDLNENDSTGLVIARKK"
     gene            complement(1228..2277)
                     /locus_tag="PLT08_06980"
     CDS             complement(1228..2277)
                     /locus_tag="PLT08_06980"
                     /inference="COORDINATES: protein motif:HMM:NF015087.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016051 - carbohydrate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate synthase family protein"
                     /protein_id="HNE04243.1"
                     /translation="MARKLQVENLAISDDNDCFVIAEIGNNHQGDLQKAMEMFKAAKE
                     CGVDAVKLQKRDNRSLLTRAAFNKQYDNENSFGVTYGEHREALEFGKYEYVELQKYAK
                     ELGLIFFSTAFDFNSADFLAELDMPLYKIASGDLKNTPLLKHIAKIGKPMIISTGGGT
                     MKDVQRAYDAIMPINKQLAILQCTASYPAEPEDLNLRVISTYREKFPDVVVGLSDHEN
                     GIAMALAAYVLGSRIVEQHFTLNHTWKGTDHAFSLEPIGMKKLVRDLRRVKVAMGDGE
                     KRIYPSEVNPIHKMGKKLVAARDLKAGQVLTREDIAIKSPSDGIPPYELDNVLGKTLA
                     KDLFEDDNFTFEDLS"
     gene            complement(2282..3112)
                     /locus_tag="PLT08_06985"
     CDS             complement(2282..3112)
                     /locus_tag="PLT08_06985"
                     /inference="COORDINATES: protein motif:HMM:NF016908.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FkbM family methyltransferase"
                     /protein_id="HNE04244.1"
                     /translation="MPEKDRIRDVIVYKVVNIFGTTLKARLMDKLPLFNKAEFYNGVQ
                     CYVSSSRELRRSQRLFLPEVRDWLEHFKPGEVFYDIGANIGMFSLTVAKMYNGHVKTY
                     AFEPSFSTFSALVHNVIGNGFGDIIFPFSLALGRDEGLHSFNYTDIASGAAVHTLDTL
                     INQTGKEFAPAFKQQVISYSLDDLVRKFQFPLPTHIKIDVDGGEMEIIQGMEESLKKT
                     GVKSVLVEVTETQEDDEQVKRLMDIFKRCGFEKTRDIPHTEYKTYPLVSDILFTKDWN
                     "
     gene            complement(3539..>4103)
                     /locus_tag="PLT08_06990"
     CDS             complement(3539..>4103)
                     /locus_tag="PLT08_06990"
                     /inference="COORDINATES: protein motif:HMM:NF025877.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Wzt carbohydrate-binding domain-containing
                     protein"
                     /protein_id="HNE04245.1"
                     /translation="IFLRDGEISNIGDIETIVQAYTQVVAEDNQTEVFFLPREDSAVN
                     FTRFSMQGSSGKLKRVFTAGEDFYFVVDYEVKEPILGDYFALLIYRTDGLLILKAAED
                     DYGRKVLEGKKPGKYKVKIRFPGNVLNEGIYKFRVVFGKRRGVFHEDRHGFSFEIEDW
                     NDYTNSSFGKRNGVLLLPLGWSEEKID"
BASE COUNT         1235 a          950 c          759 g         1159 t
ORIGIN      
        1 gctttctttg ccaattcaac agcttgttga atatccgcca ttcgtgaacg tgctaccttg
       61 aagaggactt gtccattcgc ggggttgagt ttctcaaacc actcgcccgc ttgagtaggc
      121 tgctcctgag tgccgatcca gttttttata gtaacaggaa acataatctc gtctctaaaa
      181 aacaattacc ctatttcttt cttgcaatca ccagaccagt gctatcgttt tcattcagat
      241 caccttcaag tatatcgaaa tggctggcaa caaacttcaa aacagctaaa cgttcctgtg
      301 agccatgatt cgtatccata ccgctcccat cagatttgat gtaatcaaag aaaaataacc
      361 cacctggtaa gagaagggca tgaaagtcct taaccgtatc caatggtttg ttcaagtgct
      421 caaatacagt tatacatgta attacatcca atggagggag attggcaagc ttgaattcat
      481 tttccggttt taaaattacg ggattcgcgt tttggtaccg ggcaaagcga taagctgcat
      541 agtgaaaagc aattgtctgg atatcactaa aatagacttt tatttctttt ctcacctcaa
      601 agaattcata caaggaagct gtaacgggag cagtgccgca cccaaactcc aaaatatttc
      661 gaagctttgg tttatattca aacattttct taataaggtt ggcgtaatca acaattgtct
      721 ctttataaat aagatgcaat aactgataaa ggagttcagg gcgctgccat gcttcaaggt
      781 gaatatttac atcgttcgta tcaggaatct ggttatcctg aaagcgtatt cggtataaat
      841 tcttcaaaaa atagaaatcc tgaacgccca atagtgggcg cccacccaat acgctgctcc
      901 aataattgga gaaatcttcg aaccaactcg tatccaagct ggttcgaagg gacaacttat
      961 gaataaagcg acgaagcgac aacggaacaa gaggggtttt gtaaaaacgg cgaaacaagg
     1021 ccgtatgagg aggcagttga gccccttcca aaacattcac agacagagga tgttttttat
     1081 agacttcttt cacatccaat acaagcttgt cccattgatt gaactctcta tcatttttga
     1141 aaacatctct catccaaata tctccttacg cgttttatgg agcgcccatg ctattagagc
     1201 aaacttaaag ccgcaactac tgagcgttta actaagatct tcgaaagtaa aattatcatc
     1261 ttcaaataaa tccttggcga gagtcttacc aaggacatta tccaactcat atggtggtat
     1321 gccgtcactg ggggatttga tggcgatatc ttcacgggtc aaaacctgac cggctttcaa
     1381 gtcacgtgcc gcaacgagtt tcttgcccat cttgtgaatc gggttaacct cactggggta
     1441 gatacgtttt tcaccatcac ccattgccac cttcactcga cgcaagtcac ggacaagttt
     1501 tttcataccg atcggttcga gtgagaaggc gtgatcagta cccttccatg tatggttaag
     1561 tgtgaagtgc tgttccacaa tacgtgaacc aaggacataa gcagccaacg ccatcgcaat
     1621 gccattctca tgatcggaaa gaccaacaac aacgtcagga aacttctcgc gataagtgga
     1681 gataacacga agatttagat cctcgggttc agcaggataa gaggctgtgc attgcagaat
     1741 ggccaattgc ttgttaatgg gcatgatggc atcatatgca cgctgaacat ctttcatcgt
     1801 cccgccgccg gtgcttatga tcattggctt accgatcttg gcaatatgtt taagcagagg
     1861 ggtgttcttc aaatcaccag aggcaatctt gtacagcggc atatccaact cggcaagaaa
     1921 gtcggcactg ttgaagtcga acgcggtgga aaagaagata agcccaagct ctttggcata
     1981 cttttggagc tcgacatact catacttgcc aaactccaac gcctcgcgat gttcgccata
     2041 ggtgacgcca aaactattct cgttatcgta ctgtttgtta aaggcggcgc gcgtcaacag
     2101 agatctgtta tcacgcttct gcagcttgac ggcatctaca ccacattcct tggcagcttt
     2161 gaacatttcc atggcttttt gcaggtcgcc ctggtggttg ttaccaattt cagcaattac
     2221 aaagcaatcg ttatcgtcgg agatggcgag attctctact tgaagttttc ttgccatgag
     2281 attagttcca atcctttgta aataaaatgt cagacacaag cggataagtt ttgtactcgg
     2341 tatggggaat atcgcgggtt ttctcaaacc cgcacctctt gaagatgtcc atcaatcgtt
     2401 ttacttgctc gtcgtcttcc tgcgtttccg taacttcaac aagaacagac ttaacacctg
     2461 tttttttcag gctttcttcc atcccttgaa taatttccat ttcaccacca tccacatcta
     2521 tcttgatatg agtaggcaga ggaaattgaa acttccgcac taaatcatct agtgaatagc
     2581 tgataacctg ctgcttaaaa gccggggcaa attctttgcc agtctggtta atcaaagtat
     2641 caagggtatg cacagcagcg ccagaagcga tgtcagtata gttgaaggag tgcaaacctt
     2701 catcacgccc caaggctaga gaaaatggaa aaataatatc accaaatcca ttcccaatta
     2761 cattatgcac aagtgccgaa aaagtggaga acgagggctc aaatgcgtag gtttttacat
     2821 gcccattgta cattttcgcc acagtcaggc taaacatgcc tatatttgcg ccaatatcat
     2881 agaaaacttc acctggctta aaatgctcaa gccaatcgcg cacctcaggt aaaaaaagac
     2941 gttgactgcg ccgtaattcc ctgctggacg acacataaca ttgcacccca ttataaaatt
     3001 cagccttatt aaacaacggg agcttatcca tcaaacgtgc tttaagcgtt gttccaaaaa
     3061 tattgacaac cttataaact attacatcgc gtattctatc tttttccggc attgttatac
     3121 tccttgctcg catggtttgg catcagttta ccagtcttaa atcgtacaaa caaccccacc
     3181 atattctttg taacctccat acttagatag tctttgtatg gagccgcaaa tttgaaaagg
     3241 aaacggattt ccaaagttca tatcatattg ctttgcgtta gcatatacaa aactaggaat
     3301 tttcagggaa cactcgttct caaacaacaa ttttgcggac attttgataa cagcctgata
     3361 cccccacttc tgaacaaact ttccgcacaa gcggtcttag tttcatttaa aatccttcac
     3421 tatatttgtt gtaacggttc acatagcatc atataacagg ataaagcttg gcttataaaa
     3481 aactacttaa caataaaaat atctcaatta aatgaaacct gcatggttac ggtaagtatc
     3541 agtctatttt ctcttcactc caaccaaggg gaagtaataa aacaccattt cgtttaccga
     3601 atgaagagtt ggtatagtca ttccaatctt caatctcaaa agaaaaacca tgacggtctt
     3661 catgaaaaac cccacgccgt ttaccaaaga caacccgaaa tttgtaaatg ccttcattta
     3721 acacattgcc cggaaaccgt atcttgacct tgtattttcc cggcttttta ccttcaagca
     3781 cttttctacc ataatcatct tcagctgctt tcaaaatcaa caaaccatca gtacggtaaa
     3841 tcaataatgc aaaatagtct ccaagaatag gttcttttac ttcataatca acaacaaaat
     3901 aaaaatcttc accagcagta aaaactcttt ttaatttacc cgaactcccc tgcatcgaga
     3961 aacgcgtaaa gtttactgca ctatcttcac ggggtaagaa aaagacctct gtctgattat
     4021 cttctgctac cacctgtgta taggcttgaa caattgtctc tatatctcca atatttgaaa
     4081 tctccccatc acgcaaaaag atg
//
LOCUS       DAOQJE010000213         4096 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_13320_length_4096_cov_6.713190, whole genome shotgun sequence.
ACCESSION   DAOQJE010000213 DAOQJE010000000
VERSION     DAOQJE010000213.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 4096)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4096)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4096
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13320_length_4096_cov_6.713190"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..329)
                     /locus_tag="PLT08_06995"
     CDS             complement(<1..329)
                     /locus_tag="PLT08_06995"
                     /inference="COORDINATES: protein motif:HMM:NF012343.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0009060 - aerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase subunit I"
                     /protein_id="HNE04246.1"
                     /translation="MKQYQVSPAEKKLVGSHILVAILALAVGSLFGPLQAFEHAGWDF
                     YPYLKPFIKSYYQGLTVHGVLNALVWTTFFIMGFLTFNVVFGLKRSLKNPVLNKVGFW
                     VMVVGVVT"
     gene            complement(348..848)
                     /locus_tag="PLT08_07000"
     CDS             complement(348..848)
                     /locus_tag="PLT08_07000"
                     /inference="COORDINATES: protein motif:HMM:NF024864.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit II"
                     /protein_id="HNE04247.1"
                     /translation="MSTEKLHIDPYERNWMIISAILLVLFATAVSVAAFGLGIQVPAP
                     EQRVDPKTVATDPNSPWSNPGLREIAPGKYDAYVLARAVPNWEYLPKEMSIPAGSSVS
                     FYVTSADLQHGFKIQNTNANFMVIPGQVSKLTIKFDKPGTYNFICTEYCGSGHGVMYG
                     AITVTP"
     gene            complement(845..982)
                     /locus_tag="PLT08_07005"
     CDS             complement(845..982)
                     /locus_tag="PLT08_07005"
                     /inference="COORDINATES: protein motif:HMM:NF019724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit 2A"
                     /protein_id="HNE04248.1"
                     /translation="MAKKDKEDDEFRPTGTIAVLTIFVITLIAIWATIYMILVGRGGT
                     L"
     gene            complement(1096..1683)
                     /locus_tag="PLT08_07010"
     CDS             complement(1096..1683)
                     /locus_tag="PLT08_07010"
                     /inference="COORDINATES: protein motif:HMM:NF014667.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCO family protein"
                     /protein_id="HNE04249.1"
                     /translation="MKIRFFLVGIATILVLIAGIYFLRPHTFHGTVIQSPDPSFDFTL
                     KSADGDVSISDFRGKLVLLYFGYTFCPDICPGTLANVAQALREMGAKADDVQLIMVSL
                     DPERDTPEKLKEYMGHFYPSFVGVTGSKEQLDEITSLYGVFYQKKEGSVATGYLIDHT
                     ATLMVLDREGYLKLVFPFGVTSEEIADDLKYMLRQ"
     gene            complement(1699..2373)
                     /locus_tag="PLT08_07015"
     CDS             complement(1699..2373)
                     /locus_tag="PLT08_07015"
                     /inference="COORDINATES: protein motif:HMM:NF014667.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCO family protein"
                     /protein_id="HNE04250.1"
                     /translation="MNKYALNGLYTLFGALAIAAFAFKVFQPIQVLPRMRLSPAYSFI
                     NQDGETVTSEDLRGQFVLYSFTYTNCPAPCYNMGETMKEVQARLNEVDLGGIPVSFVT
                     ISIDPDRDTPEALKTYGTDLGANFDEWMFVTTTNKALLKTIVGSGFETYYEDKGNGSF
                     ALDPGYVLVDGWGIIRAEYRYSTEVSDADRIIRHMGVLADEVKNSKGSTKLAYEAAHL
                     FLCYAP"
     gene            complement(2370..2876)
                     /locus_tag="PLT08_07020"
     CDS             complement(2370..2876)
                     /locus_tag="PLT08_07020"
                     /inference="COORDINATES: protein motif:HMM:NF014173.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HNE04251.1"
                     /translation="MFRVNRQTDYAVRVVLALAKKPQGTRLPTAEIGREMLIPPSLLQ
                     RIVAELANGGYIKTQPGRDGGVSLAHLPSEITLLQVVEHFEGNLVISDCILKEGDCPF
                     ETKCPVSCQWKRLNDLLIAEMSRITFEQLVEDGKQIETSQMTTTASNQLKVVSNQKTF
                     SLTDSLSA"
     gene            3132..>4096
                     /gene="lgt"
                     /locus_tag="PLT08_07025"
     CDS             3132..>4096
                     /gene="lgt"
                     /locus_tag="PLT08_07025"
                     /EC_number="2.5.1.145"
                     /inference="COORDINATES: protein motif:HMM:NF013915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HNE04252.1"
                     /translation="MIDPVIFSFKLFTWQVTLTWYGLIVMSGVLVAAWFAEREVRRRG
                     ENGEVLIDAMVWAVVAGIIGARLWYVMNALIGGNRSYIEDPMSIIRPPIAGLHFFGGL
                     LFGAIALIIYLRNNGYDVWLFMDAVAPVALLGQAVGRIGNYINQELYGPPTQLPWGIS
                     IPADHRLAQYADLAKYPVESTRFHPTFAYEMVLNVLAFLFIMWYARRDEEEPKPGTLF
                     SMWLIAAGFIRTFIEFFRPDQPRLGDTFITTSMLVAFLMGVTGVILFLARHGKLQIAA
                     LTDWEEEYFIKKVEKKSAPRARKVESAAVVETDSEPEVDEPVVKR"
BASE COUNT         1040 a          895 c         1034 g         1127 t
ORIGIN      
        1 gtgaccacac caacaaccat tacccagaaa ccgactttgt tcaagacagg attcttaagt
       61 gagcgtttga gaccaaaaac aacattgaag gtcaaaaagc ccatgatgaa gaaggtcgtc
      121 caaaccaagg cgttcaacac accgtggacg gtaagtccct gatagtacga cttgatgaac
      181 ggtttgaggt aagggtagaa atcccaaccg gcatgttcaa aggcttgcaa aggaccgaac
      241 aagctgccaa ccgcaagtgc gaggatagca accaaaatgt gggagccgac aagtttcttt
      301 tcggcagggg atacttgata ttgtttcata gttgactctc tttgcactta tggtgtaacg
      361 gtgatcgcgc catacatcac gccgtgacca ctgccgcaat attcggtgca gatgaagttg
      421 taggtgccgg gcttatcgaa cttgatggtc agtttagaga cctgtccggg gatgaccatg
      481 aaattggcat tcgtgttctg gatcttgaac ccgtgctgaa gatcagcaga ggtcacatag
      541 aaagagacgc tggaaccagc aggaatgctc atttctttag gtaaatattc ccagttggga
      601 actgcacgtg caagcacata agcatcatat ttaccggggg caatttcgcg caagccaggg
      661 ttcgaccagg ggctgttcgg gtcggtggca accgtcttcg ggtccacgcg ttgttcaggt
      721 gcagggacct gaatgcccaa accaaaggct gctacactca cggcagttgc gaacaatacg
      781 agcaggatgg cgctaataat catccaattg cgttcgtatg ggtcaatatg caatttctct
      841 gtgctcatag tgtgcctcca cgtccgacaa ggatcatata gatcgttgcc cagattgcga
      901 tgagtgtgat cacgaatatc gtcaagactg caatggtgcc agtcgggcga aactcatcgt
      961 cttctttatc tttcttcgcc atgttacatt ctcctcttga aaaaatgaaa ttaggatgac
     1021 taccgcttca cgcggcgccg caagctgcgg tggtggataa tgggcagttc tctacgctgt
     1081 agcaggtcca cctccttatt gccgtagcat gtacttcaga tcatcggcaa tctcttcaga
     1141 agtgacgccg aatggaaata ccaacttcaa atatccttcc cgatccaata ccattaaagt
     1201 agctgtatgg tcgatcaaat acccggtagc aacactgcct tccttctttt gatagaagac
     1261 cccatacagt gaggtaattt cgtctagttg ttctttgcta cctgtaacgc ccacaaacga
     1321 agggtaaaaa tgccccatgt attcctttaa cttttcaggg gtatcccgtt ctgggtcaag
     1381 cgagaccatg atgagctgaa catcatcggc ttttgccccc atttctcgca aagcctgagc
     1441 cacattcgca agcgtgccgg ggcaaatatc tgggcagaag gtgtaaccga aataaagcaa
     1501 taccagtttg ccgcggaagt cgcttatgga aacatcgccg tctgcgcttt taagggtgaa
     1561 atcaaaagaa gggtcagggg attgaatgac cgttccatga aatgtatgtg ggcgtaaaaa
     1621 atatattcct gctatcaaaa caagaattgt cgcaatacca acaagaaaaa atcggatttt
     1681 cataataacg atggaccttt atggcgcata acaaaggaag aggtgagccg cttcatatgc
     1741 tagtttggta gaacctttgc tgttcttaac ttcatctgcc agaaccccca tatggcggat
     1801 gatacggtca gcatctgaga cttcagtgga gtagcgatat tcagcacgga tgatgcccca
     1861 gccatctaca agaacgtaac cggggtcgag ggcaaaacta ccatttcctt tgtcttcgta
     1921 atatgtttca aaacctgagc caacaatggt cttgagtaaa gccttgtttg tggtggtgac
     1981 gaacatccat tcgtcaaagt ttgcaccgag gtctgtgccg taagttttca acgcttcggg
     2041 cgtatctcgg tctgggtcga ttgagatggt gacaaatgaa accggaatac cgcccaaatc
     2101 cacttcgttg aggcgggctt gtacttcttt catggtttcg cccatgttgt agcaaggtgc
     2161 tgggcagttg gtgtatgtga aggagtagag gacgaactgt ccgcgtaggt cttcgctggt
     2221 aaccgtttcg ccatcttggt tgataaaact ataagcaggg gagaggcgca tgcgggggag
     2281 tacttgaatg ggctgaaaaa ctttaaatgc gaaagcagca attgcaagag caccaaagag
     2341 tgtgtacaga ccgttgagag cgtatttgtt catgcagaga ggctgtcagt gagagaaaag
     2401 gttttttgat tagaaacaac tttgagttgg tttgaggcgg ttgtcgtcat ttggctggtt
     2461 tcgatctgtt ttccgtcttc taccaattgt tcgaaggtga tacgagacat ttctgcgatc
     2521 agtaggtcat ttaagcgctt ccattggcag cttaccgggc atttcgtctc aaaagggcaa
     2581 tcgccttcct ttaatatgca gtctgagatg accaaattgc cttcaaagtg ctctacgacc
     2641 tgtaaaaggg tgatctcgct ggggaggtga gccaagctga caccgccgtc gcgcccaggc
     2701 tgagttttga tgtagccgcc gtttgccaat tcagccacaa tgcgttgaag caaagaaggc
     2761 ggaatgagca tttcgcgtcc gatttctgca gtaggcaaac gagtcccttg cggttttttc
     2821 gcaagagcca gcacaacgcg aacggcataa tcggtttggc ggtttactcg gaacattaat
     2881 atcctttggt ttaacattga catttgatgt caatgtttac tgagattgta aggatttggg
     2941 gctttctttt gacgtgttat taatcacatt gttaaatgac aaacgtctcc tattcttgtc
     3001 agcacgtgaa gtatttatga cagaatttgt tgtatttagc aaaaagtaac atatgccctt
     3061 ctattaggct gtatgtattg cacgatttga tttggggata taatcgtcaa aacttaactc
     3121 agaggtaata catgatagac cccgttattt tcagttttaa attattcacg tggcaagtca
     3181 cactgacatg gtatggcttg atcgtgatgt ctggtgtgtt ggttgccgct tggtttgccg
     3241 aacgtgaagt gcgccgccgt ggagagaatg gtgaagtatt gatcgacgcg atggtgtggg
     3301 cggttgtagc tggcattatt ggtgcgcgct tatggtatgt catgaatgcg ctgattggtg
     3361 gcaatcgttc ttatattgaa gaccccatgt ccattattcg cccgccaatc gctggtttac
     3421 atttctttgg tggtttgctt tttggtgcaa ttgctctcat catttatctg cgcaacaacg
     3481 gatatgatgt gtggctgttc atggacgcgg ttgcgcccgt ggcattgctt gggcaggctg
     3541 taggcagaat tggtaattac attaatcagg aactgtacgg tccgccaaca caacttccgt
     3601 ggggtatttc gatccctgct gatcatcgcc ttgctcaata tgcagatttg gcgaagtacc
     3661 cggtagagtc aacgcgcttt catccgacct ttgcttatga aatggtcttg aatgttttgg
     3721 cttttctttt catcatgtgg tacgcccgtc gtgatgagga agagcccaag cctggcactc
     3781 tctttagcat gtggttgatc gctgctggat tcattcgtac gtttattgaa tttttccgcc
     3841 cagatcaacc gcgccttggc gatactttta ttaccacctc catgttggtt gcgttcctca
     3901 tgggcgtaac tggtgtgatt ttgttcctcg ctcgccatgg caaacttcaa attgcagcat
     3961 tgacagattg ggaagaagag tatttcatca agaaagtgga aaagaagtct gcgcctcgcg
     4021 ctcgcaaagt agaatctgcg gctgttgtgg aaacagatag cgaacccgaa gttgatgaac
     4081 ccgttgtaaa acgaaa
//
LOCUS       DAOQJE010000214         4079 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_13387_length_4079_cov_5.461481, whole genome shotgun sequence.
ACCESSION   DAOQJE010000214 DAOQJE010000000
VERSION     DAOQJE010000214.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 4079)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4079)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4079
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13387_length_4079_cov_5.461481"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..373)
                     /locus_tag="PLT08_07030"
     CDS             complement(<1..373)
                     /locus_tag="PLT08_07030"
                     /inference="COORDINATES: protein motif:HMM:NF013143.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HNE04253.1"
                     /translation="MNWLLEPLTYEFMQRGLLASVIVGVLCAVMGTYVVLRGMAFLGD
                     AMAHAILPGVAIAYILKGDLLIGAGVAAVAVALTIGLFTKDGAVKEDTAIGILFAAAL
                     SLGVALISTMQTYAVDLSHILF"
     gene            complement(370..1176)
                     /locus_tag="PLT08_07035"
     CDS             complement(370..1176)
                     /locus_tag="PLT08_07035"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HNE04254.1"
                     /translation="MSHTPHRLEVKNISIGYGDKTILRDLSFQVPHGSRVAVVGPNGA
                     GKSTLFKALVGLLPLQQGEIHIHGEKLGTHQDCVAYVPQREEVDWRFPVTVDDVVMMG
                     RFSQMGWFKRATLRDKQAVQKSLAQMGIADLAGRSIGQLSGGQQQRAFLARAIAQEPH
                     ILLMDEPFTGVDITTQEATLRLLDHLQEKEVTAIVSTHDLNLAGSRFDLVLLLNHRLI
                     AFGTPKEVFAKENLAQAFGNSLLVMDNGMMLVDECCPPDEEHTHHHTEAK"
     gene            complement(1247..2452)
                     /locus_tag="PLT08_07040"
     CDS             complement(1247..2452)
                     /locus_tag="PLT08_07040"
                     /inference="COORDINATES: protein motif:HMM:NF014542.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HNE04255.1"
                     /translation="MEKDSRLPVTVLSGFLGSGKTTVLNHILNNREGLHVAVIVNDMS
                     EVNIDAQLIRDGGAELSRTEEKLVEMTNGCICCTLREDLLLEVTRLAGEKRFDYLLIE
                     STGIGEPLAVAATFDFPMNEDDTNLSDIARLDTMVTVVDASNWLRDYLEAKSLKELGM
                     AVSDEDGRTMVDLLIDQVEFADVILVNKVDLVTEEEIGKLIAILKKLNPDAAVVKTEF
                     GKAPLNFILNTGLFNMEKSAALPQWADELAGRHNPETEEYGISSLTYQANLPFHPERL
                     MEFLQNGGLDSIVRSKGYFWLASRMDHALGWSQAGTQTTINLAGLWWAAVPKKQWPRA
                     AELRKFIQSNWQEPYGDRRQELVFIGQKLQKDELKQALDQCLMTEMEMKNIKKLKITD
                     PFPSLQPNN"
     gene            complement(2465..2707)
                     /locus_tag="PLT08_07045"
     CDS             complement(2465..2707)
                     /locus_tag="PLT08_07045"
                     /inference="COORDINATES: protein motif:HMM:NF013026.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bL28 family ribosomal protein"
                     /protein_id="HNE04256.1"
                     /translation="MARKLNHKTRKALAPKTRFGKNISFSQRHTPRKFKPNVQTITLW
                     INGKPIRVKLSARQIRSLGKQEQPKKLMTELRKLAK"
     gene            complement(2707..2871)
                     /gene="rpmG"
                     /locus_tag="PLT08_07050"
     CDS             complement(2707..2871)
                     /gene="rpmG"
                     /locus_tag="PLT08_07050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013584775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HNE04257.1"
                     /translation="MAKKKDIRIVVKMQSTESQYMYYTQKSRRNNPQRIELRKFDPIV
                     KKHVLFKESK"
     gene            complement(2966..3904)
                     /locus_tag="PLT08_07055"
     CDS             complement(2966..3904)
                     /locus_tag="PLT08_07055"
                     /inference="COORDINATES: protein motif:HMM:NF013465.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein"
                     /protein_id="HNE04258.1"
                     /translation="MKKIFNTIIVTLTFSALLLAGCGSAPQSGSDTLKVLASTTFLAD
                     IAQNVAGDRVQVESLLPVGADPHAYQAAPADVSKIAESNVLILNGVEYEHFIEPLLEN
                     AGGERLVIEATTGLTPHQMEEHAAEAESGTSHEHEAGDPHMWLDPNLVVTYVENIRDG
                     LSKADPDGAETYAANAEAYIAQLKDLDAWISEQVNSIPAENRLLVTNHEALGYFAERY
                     GFEVVDTILPSFSSEASASAQEIAAAVDAVKNSGAPAIFLGEVENADLANQIAAETGV
                     KVVDTLYLESLTDGEPAATYIDMMKHNVTQIIEGLK"
BASE COUNT         1002 a          982 c          968 g         1127 t
ORIGIN      
        1 cgaataggat gtgagacaag tccacggcgt aggtttgcat ggtgctgatg agcgccacac
       61 ccagggatag cgccgctgcg aacaaaatac cgatagcagt atcttctttg acagctccat
      121 ctttggtgaa gagaccgatg gtgagagcaa ctgcaacagc ggcaacgcct gcgccgatga
      181 gcaagtcgcc tttcaagatg taggcaatgg ctacgccggg gagaatggca tgcgccatcg
      241 cgtcgcctag gaatgccata ccgcgcaaga caacatacgt tcccatcacg gcacacagca
      301 caccaacaat aacggaggcg agcagtccgc gttgcatgaa ttcgtaggtg agaggttcaa
      361 gcaaccagtt catttcgcct cagtgtgatg atgagtgtgt tcttcgtctg gagggcagca
      421 ttcatccacc agcatcatgc cgttgtccat cacaagcagg gagtttccaa atgcctgagc
      481 aagattttct ttcgcgaaga cttctttcgg cgtgccaaac gcaatgagac ggtgattcag
      541 caaaaggaca aggtcgaagc gtgagcctgc gagattcagg tcatgagtcg agacgatggc
      601 agtcacttct ttttcttgca agtggtcaag cagacgcaat gtcgcttctt gagtagtgat
      661 gtccacaccg gtgaagggtt catccattaa aaggatgtgt ggctcttgag cgatggcacg
      721 tgctagaaaa gcgcgttgtt gttgcccgcc cgagagctga ccgatcgaac gaccagccaa
      781 atctgcgatg cccatttgag ccaggctttt ttgaacggct tgtttatcgc ggagtgtcgc
      841 tcgtttgaac caacccattt gagagaaacg ccccatcatc actacatcat ctactgtaac
      901 ggggaaacgc cagtccactt cttcgcgttg aggcacatac gcaacacaat cttgatgtgt
      961 gcctaacttt tcaccgtgaa tatggatctc accttgttgt aaaggcagaa gcccaaccaa
     1021 ggctttgaat agtgtggact tgcccgctcc gtttggaccg acgacagcga cgcgcgaccc
     1081 atgcggcact tgaaaactca aatcccgcaa aatggttttg tcgccgtagc cgatgctgat
     1141 gttttttact tcaagacgat ggggtgtatg agacatttgt atcgaatgac gctttcaatt
     1201 ttattctatc ttacttctag ttgaacatag atgtatcaga ttagtgttag ttatttggct
     1261 gaagagacgg aaatgggtct gtgatcttta atttcttgat atttttcatc tccatttcgg
     1321 tcattaagca ttgatctaat gcctgcttta attcatcctt ttgcagtttt tgaccaataa
     1381 agacgagttc ttgtctgcga tcaccatacg gctcctgcca attggattga atgaattttc
     1441 tcaattcagc cgctctgggc cattgctttt taggaacagc tgcccaccat agccctgcca
     1501 gattgatggt tgtttgagtg cctgcctgtg accaacccaa ggcatgatcc atacgagatg
     1561 caagccaaaa gtagcccttt gagcgcacaa tactatccaa cccgccgttc tgtaaaaatt
     1621 ccataaggcg ttctgggtga aaaggcaggt tggcttgata ggtaaggcta ctaattccgt
     1681 attcttccgt ttcggggttg tggcgccctg ccagttcatc tgcccattgt ggcagcgcag
     1741 cagatttttc catgttgaat agcccggtat taagaatgaa attcaatggt gctttaccaa
     1801 attctgtttt gaccacggct gcatctgggt tcaacttctt gagaatggcg atcaatttcc
     1861 ctatttcttc ctcggtaacg aggtcaactt tattgaccaa gatcacatca gcaaactcaa
     1921 cctgatcgat gagtaaatcc accatggtac gaccatcttc atcgctgact gccatgccca
     1981 attccttcaa gcttttggct tcaaggtaat ctcgcagcca gttggatgca tccacaacgg
     2041 ttaccatggt atcgaggcgg gcaatgtcgg agagattggt atcatcttca ttcattggga
     2101 aatcgaatgt cgcagccact gccagcggct caccgatccc agtcgactca atgagcagat
     2161 aatcaaaccg cttttcgcca gcaagccggg taacttccag gagcaggtct tcgcgcaggg
     2221 tacaacagat acagccattg gtcatttcga ccagtttctc ttctgtgcgg ctaagttctg
     2281 cgccaccatc gcggatcagt tgcgcatcga tgttgacttc gctcatatca ttgacaataa
     2341 ccgccacatg tagaccttca cggttgttga gaatatggtt tagcacagtg gtttttcctg
     2401 agcctaaaaa gccggaaagc acagtaacag gtaaacgaga atctttttcc atggtgcaat
     2461 ccttttattt tgcaagctta cgaagttctg tcattaattt tttaggttgt tcttgtttgc
     2521 caagcgaacg gatttgcctg gcagataact tcacacggat tggctttcca ttgatccata
     2581 acgtaatggt ttgtacgttc ggcttgaatt tacgtggagt atggcgttga gaaaaactta
     2641 tattctttcc gaaacgtgtt ttgggagcta acgccttgcg ggttttgtga ttcaatttgc
     2701 gtgccattat ttgctctcct tgaataaaac gtgctttttt acgatcgggt caaacttgcg
     2761 aagttctatc ctttgagggt tgttgcgacg gctcttctgg gtgtaataca tgtattggct
     2821 ttctgtgctt tgcatcttta caacgatgcg gatgtctttt ttctttgcca tgtgtgcctc
     2881 taaatgaaaa tgattttcaa taagaaattc gataaaaaga cagggagcct atgaatctcc
     2941 ctgtctcaga gaaaattaca gagtcttact tcaaaccttc gatgatctgc gtgacattat
     3001 gcttcatcat gtcaatgtag gtcgcagcgg gctcaccatc agtgagtgat tcaagataaa
     3061 gcgtatctac cactttgaca cccgtttcag cggcgatctg attggcaagg tctgcatttt
     3121 ctacttcgcc gaggaagatg gcaggcgcac cagaattctt gaccgcatcc accgccgcag
     3181 cgatctcttg agcagaagcg cttgcttcag aactaaagct cggcaaaata gtatccacaa
     3241 cctcaaagcc atagcgttct gcaaaataac ccaacgcctc atggtttgtg accaacagac
     3301 gattttctgc cggaatgctg tttacctgtt cgctaatcca tgcatccaag tctttcaact
     3361 gcgcaatata agcttcggcg ttggcggcat aggtttctgc accgtcaggg tctgctttgc
     3421 tcaagccgtc acggatattc tctacataag tgacgacaag gttcgggtcc agccacatat
     3481 gcggatcgcc cgcctcatgc tcgtgacttg tgcctgattc tgcttcggcg gcatgttctt
     3541 ccatctgatg cggggtgagc ccggtggtgg cttcaatgac caagcgttct ccgcctgcat
     3601 tttccagcaa cggctcgata aagtgctcat actccacgcc attgagaatc agcacattgc
     3661 tttctgcgat cttggaaaca tcggctgggg cagcctgata ggcatgcgga tctgctccaa
     3721 caggaagcaa ggactcaacc tgcacccggt caccagctac gttctgggca atgtctgcca
     3781 ggaaggtggt agaggcgaga accttcaagg tgtcgcttcc gctttgtggg gcagacccac
     3841 acccggcgag gagaagagcc gagaaggtta atgttacgat aatggtattg aaaatctttt
     3901 tcatttagaa ctcctaaaat ttatccctca acatgtgagg gagttttttt atcactcaga
     3961 tggggctaat tcacatcatg agcaaaattt atttgcaatt cgtacacaag ccaaacagtt
     4021 gaagccaatg ttcctgaatt tgataaccgg tctttttggc aatcgacttg gttaagggt
//
LOCUS       DAOQJE010000215         4068 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_13431_length_4068_cov_5.316721, whole genome shotgun sequence.
ACCESSION   DAOQJE010000215 DAOQJE010000000
VERSION     DAOQJE010000215.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 4068)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4068)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4068
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13431_length_4068_cov_5.316721"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            518..>4068
                     /locus_tag="PLT08_07060"
     CDS             518..>4068
                     /locus_tag="PLT08_07060"
                     /inference="COORDINATES: protein motif:HMM:NF024907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FG-GAP-like repeat-containing protein"
                     /protein_id="HNE04259.1"
                     /translation="MYIRKPFIIIILTIASFLPKNVMATPMQRPNSSIQATLIGELDT
                     GFGVNGVVNTDFFSGSEQVWDVIVTLDGSIILAGSATDSGGDTGFGIAKYNSSGALDA
                     SFGSGGKLLMQISGSVDRATTVLPLSDGKILVGGWGGVSGSRDFIIIKLNSNGSQDTS
                     FGINGIIQTDINNNGSDDFIASLNIDSFGRIVAVGQKDHTVAIARYMSNGDPDATFSG
                     DGKTSFAILNNTSLWDSVIDAAIQSDNKIIIVGGKETAGSYDFALARVNENGSIDTTF
                     HSDGFVTTTIGSGVSVIKSVVVQPDGKILVAGRGSGNTKHALARYLANGQPDTSFDVD
                     GIALYDFNTTGGDDVASDMVLLDNGNILTIGFTETTSNVFDIVVSSFLPNGALNPEFS
                     SDGYTVTPGTGFDMGTSFLAAAAVQTDGKIIFAGTIGTGSSANFGVARFHGLNPVTFT
                     EHQIPGNFYYAIDTGDIDNDGDIDIVSATDNGNNLVWLEKVLNTWSSHTIKTGLIPGV
                     GGGARDTLLADIDNDTDLDIVLASTGEDKLYWFENNGSPGNSWTEHLLAQTYDTPINI
                     SVSDLEGDGDLDIFAANLYSDNISWLENEIDPVLGNVFIERVVVSSWWGPYDLHIYDI
                     DGDGDNDIIASSFFSYQVNWFENDGSPNAGSWPIHNIYTSNTDIPSISISDIDNDGDY
                     DVAVIELTGTNRSIYWMENIGLPGNATNWQKNIIQSGIDWGRFIATSDLNRDGNIDII
                     RAHSSTAPTVSWFANLGDPVSGGWSDLLINNSNGFAQLKVDDISSDGRVDIIAAPTSG
                     SIYWWEQKNDLPLQNTPTISDIQVFDRMPIDYSCVPENIGPTGPIIPILVSADVYFKI
                     TNPELIDTVYANIKAGGAPIYKSYPMLLSSLKTEVPDIYKYPIRIPDYRSLLRGLFEF
                     LFELIKEKAEIYLPYEINVSDWALQNIDIPLTANIENLVLIEKNGNTHTLAPSAPIVV
                     TTINHRPLEPSSNAGIYKIACPMSFVKLESPADILLTSNEGMRVGNTSTGVVSEIPGA
                     FYTLPDYEHEFIIIGSPDPSEDFQIKITGTDNGTYGLSYGYLGDSSTLFTRTDYINLP
                     TTINHNDYYINPVVPSIISIKTNSPNPTNAVGVDYLVTFSEEVKDIEISDFNITKSSG
                     ITNDSITAVTGSGSTRIVTINTGSGYGTLRLDIPDTAS"
BASE COUNT         1258 a          785 c          855 g         1170 t
ORIGIN      
        1 cgatcgatga caacgacgat ggcggtgcgg acttcatgca gttcttcagc ggcaatgctc
       61 ccgcccccag ccgtcccaaa ctcatccttc aatattacct gccgtagaag taacatctaa
      121 aaaagcggac ttgccatacg gcaagtccgc tttgcttgtt tgtacatgag caagcaaaat
      181 cggcattcat gccaaattgt agttttcaaa gaatgtccaa taaaatctcg ttcgttttta
      241 ctctggagaa atcatcgcct accttgtacg aaggaagcca ttaagcgatt ttattgttaa
      301 gttgtggtgc tatagcaaag ggatctggat ttgtcaatat ttatgataat tttctagtat
      361 attcagatcg ccgtatgtca actttaaacc attacatctt cctaattgat tttgctacaa
      421 aaatccttat agttattttt atcattgtgt gttatcagaa cacaaagttg ctccatgtaa
      481 caggttgagc catcattcat tcaaatagga gacgaggatg tatatcagaa agcccttcat
      541 tattattatt ttgacaatcg caagtttcct acccaaaaat gtcatggcaa cgccgatgca
      601 aaggccaaat tcatcaatcc aagcaacttt aattggagaa ttggataccg gttttggtgt
      661 caacggtgta gtcaatactg atttttttag tgggtcagag caggtttggg atgtaatcgt
      721 taccctggat ggaagtatta ttttggcagg atcagcgact gactcgggcg gtgatacagg
      781 ttttgggata gccaaataca attcctcagg cgctttggac gcctcctttg gttccggcgg
      841 caaactatta atgcaaatat caggaagcgt ggatcgagct acaactgtat tacccctttc
      901 tgacggcaaa atactagttg gaggatgggg aggggtaagc ggctcaagag atttcattat
      961 aattaaatta aattcaaatg gtagtcaaga tacaagtttt gggataaatg gaattattca
     1021 aacggatatt aacaataacg gcagcgatga ttttattgcg tcattgaaca tagattcttt
     1081 tggaagaata gttgcagttg ggcaaaagga ccatactgtt gccattgcta ggtacatgag
     1141 caatggggac cccgatgcaa cttttagcgg cgacggcaag acaagttttg caatattaaa
     1201 taacacaagt ctttgggata gcgttataga tgcggcaatt caatcggaca ataaaattat
     1261 tattgttgga ggaaaagaaa cagctggttc ttatgacttt gctcttgcaa gagtgaatga
     1321 aaatgggagt attgatacaa catttcattc cgatggcttt gttactacga ccatcggttc
     1381 cggggttagc gttatcaaat cagtggtcgt tcaaccagat ggaaaaattc ttgttgcagg
     1441 ccgagggtca ggtaacacaa aacacgctct cgccagatat ttagctaatg gacaaccaga
     1501 tacgagtttt gacgtggatg ggattgcgct ttatgatttc aacacaactg gcggtgacga
     1561 cgtcgcgtcg gatatggtgc ttttagataa tgggaatatc ctgacgatag gattcacaga
     1621 aacaacctcg aacgtctttg atatagttgt ctccagtttt ttgccgaatg gagcgctgaa
     1681 ccctgagttc agttcggatg gatatacggt aacaccagga acaggatttg atatgggaac
     1741 cagttttctt gctgcagcgg cagttcaaac tgacggcaaa attatttttg ccggaacaat
     1801 aggaactgga tcatcagcta attttggagt tgcacggttt cacggattga atcctgtgac
     1861 ttttactgaa catcaaatac ctggaaattt ctattacgca atcgatacag gcgacattga
     1921 caatgacggg gatatcgata ttgttagtgc aacagacaat ggaaacaatc tggtatggct
     1981 agagaaagta ttaaacactt ggtcaagcca tacaataaaa acaggtttaa tacccggcgt
     2041 aggaggcgga gcaagagata ctttactagc agatattgat aatgataccg atctggacat
     2101 tgttctagct agcaccggcg aagataaatt atattggttt gaaaacaatg gatctcctgg
     2161 aaatagttgg acagaacatt tgttagcaca gacatacgac acaccaatta atatttcagt
     2221 ttcagaccta gaaggcgacg gcgatctaga catctttgca gcaaatttat attcagacaa
     2281 tattagttgg ctcgaaaatg aaatagatcc ggtattagga aatgtcttta ttgaacgagt
     2341 tgtcgtttct tcttggtggg gtccatacga ccttcatatt tacgatattg atggtgatgg
     2401 tgataacgac ataatcgcct catcattctt ctcctatcag gtaaattggt ttgaaaatga
     2461 tgggtcgcca aatgctggat catggccaat tcataatata tatacttcaa acaccgatat
     2521 acccagcatt tccatttctg atattgacaa cgacggggat tatgatgttg ctgtaattga
     2581 gttaacagga acaaaccgat cgatatattg gatggaaaat attggtttac cgggaaacgc
     2641 aacaaattgg caaaaaaaca taattcaatc cggtattgat tggggaagat ttattgcaac
     2701 cagcgactta aacagggacg gaaatataga tattattagg gcacactcca gcactgcgcc
     2761 tacagtttct tggttcgcca atttgggaga tccagtctcc ggcggctgga gcgatctatt
     2821 aataaataat tccaatggat ttgcccagtt gaaggttgat gatattagtt cagacggcag
     2881 agttgatatt attgcagcac caacttctgg gagtatttat tggtgggagc aaaagaacga
     2941 tcttccatta cagaacacac ctaccattag cgacattcaa gtttttgaca gaatgcccat
     3001 tgattattcc tgtgtcccag aaaacatagg tccgacagga ccaataattc ctattctcgt
     3061 tagtgcagat gtttatttca aaatcaccaa tccagaatta atagatactg tctatgcaaa
     3121 cattaaggcg ggaggtgctc ctatttacaa atcttaccca atgctccttt cttctttaaa
     3181 aacagaagtg cctgatatat ataagtaccc aataaggata cctgattaca gaagcctgct
     3241 tcggggatta tttgaatttt tatttgaact aattaaggaa aaagcagaaa tctatttgcc
     3301 ctacgaaata aatgtctcgg attgggccct gcaaaatatc gatattccac taactgcaaa
     3361 tattgaaaat ttagttttga ttgaaaaaaa cgggaacacc cataccttag caccttcagc
     3421 gcctattgtg gtcaccacaa taaatcatcg tccgcttgag cctagtagta atgcaggtat
     3481 ttacaaaatt gcatgtccga tgtcattcgt gaaattagaa tctcccgcgg atatactttt
     3541 aacaagcaat gaaggaatga gagtcggaaa cacatcaaca ggtgtagtat ctgaaattcc
     3601 tggcgctttt tacacattgc ctgattatga gcacgaattt attatcatag gtagtccaga
     3661 ccctagtgaa gattttcaaa taaaaattac tgggacagat aacggcacat acggtctctc
     3721 ttatggctac ttaggggata gtagcacctt atttacaaga acagattata taaacttacc
     3781 aacaaccatt aatcataatg attactatat caaccccgtc gtacctagta tcatatctat
     3841 aaaaaccaat tcccccaacc ctacgaatgc tgtaggtgtg gactatttag taactttctc
     3901 agaagaggtg aaagatattg aaatttccga ttttaatatt acaaaaagca gtggcattac
     3961 aaacgattca atcaccgctg tcaccggctc aggttccacg cgaattgtca ctatcaatac
     4021 cggctctggc tatggcactc taaggctgga catcccggac accgcctc
//
LOCUS       DAOQJE010000216         4063 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_13460_length_4063_cov_6.212325, whole genome shotgun sequence.
ACCESSION   DAOQJE010000216 DAOQJE010000000
VERSION     DAOQJE010000216.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 4063)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4063)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4063
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13460_length_4063_cov_6.212325"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..505
                     /locus_tag="PLT08_07065"
     CDS             <1..505
                     /locus_tag="PLT08_07065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HNE04260.1"
                     /translation="MDSITRLARAYNLTVNPSGRTLSGGMDPSALYPPKRFFGAARNV
                     EEGGSLTIIATCLVDTGSRLDDVVYEEFKGTGNMELHLSRKLQERRTFPAVDLERSST
                     RREDLLLGPDLLQRVWLLRRMFVQMTSSQPAGAGMDPSVATEAILTRLEKSKNNIEFL
                     ENLTKDA"
     gene            552..1727
                     /locus_tag="PLT08_07070"
     CDS             552..1727
                     /locus_tag="PLT08_07070"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HNE04261.1"
                     /translation="MKLFERIKSIFPARKKAKEEEQPQAVSAVNEPSTSPRKEVKKAD
                     RFTIVSWSVTSVLVVALLSGTLYYKSTLPATVVTVPAATEEPAADGSVPVVVNSGTGG
                     GGSEGLFPTILRKLQIKTNKPERPRYDPELYRVVRGDSMYRIAEDFKIKSETILYVND
                     QLEDNPHSLRPGMELTIPPADGLYYTWKEGDTFESVAEEYNANADDIINFTGNQIDLT
                     DPKVEVGTQIFIPGGSRELVNWAAQLPTNARGVNTGTGGGNAANVCGGGPVASGFGWP
                     ADDHTLSGNAYGPGHLGIDISAPEGANVYAAGTGVVTMAAGGWNYGYGNVVQIDHGNG
                     YVTVYAHLSSILVSQCQTVGQGSVIGLAGNTGNSFGAHLHFEIRVGGSNINPYDIVQ"
     gene            1774..2553
                     /locus_tag="PLT08_07075"
     CDS             1774..2553
                     /locus_tag="PLT08_07075"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR00121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004077 - biotin-[acetyl-CoA-carboxylase]
                     ligase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HNE04262.1"
                     /translation="MNESSLKKRLAKLNLGGLRYFESLGSTNDEALAWAARGADDFSL
                     VVADEQTQGRGRLDRKWFTPKGSAIAMSLILRPTAVMRSHLSRTVGLAALSLADACLK
                     LGLSPKIKWPNDILLDSKKTAGILVEMVWAGEDADSLVIGMGINVHKSAVPPRETLQF
                     PATSLEDMLGNAPKREDIIFQILSNVQRWREQIGTERFIQAWEEKLAFRGEAIQIHAG
                     GAESHQGVLDGLESDGSLRICDEHNKSVIVRFGDVSLRPTA"
     gene            2584..2808
                     /locus_tag="PLT08_07080"
     CDS             2584..2808
                     /locus_tag="PLT08_07080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE04263.1"
                     /translation="MFDDLRNDAAKQYEDEVQAEYQPASVTSSGAPRQRSTKFLGMTS
                     IQRFILVFLLMTATCVIGFLCLFAFGKIAF"
     gene            2860..3624
                     /locus_tag="PLT08_07085"
     CDS             2860..3624
                     /locus_tag="PLT08_07085"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HNE04264.1"
                     /translation="MQILNTLNFFTSKNLFKSYKRLGFWQTIILFSGVAFVSVTGIIV
                     PTNMLLGGSIRTGMVINFIVCTTFMPYHLHQILSLMVELDTLRMNIYDKSIRDELTKA
                     YNRRYFFEASKAIENNEALIPQNTSLLLIDIDDFKLLNDTYGHDIGDLALKTLTEESS
                     RLLRTTDVFARYGGDEFICLLPNTKQSQAYEIATRLLESVNKHPFYKKDGNIFLRLSI
                     GIATTTSETKLNDLISLADKALYKSKQNGKNTVEAL"
     gene            complement(3665..>4063)
                     /locus_tag="PLT08_07090"
     CDS             complement(3665..>4063)
                     /locus_tag="PLT08_07090"
                     /inference="COORDINATES: protein motif:HMM:NF015922.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase C-terminal beta-propeller
                     domain-containing protein"
                     /protein_id="HNE04265.1"
                     /translation="SMDVVDKEGALLVVTTNGFGKQTPLKEYTPKGRATGGISTIDQK
                     ALQEIGKIAAARVVNKDDDLTIMTANGVAIRLKVKDVKQSGRATRGVHLIKPQEGDSV
                     ASVARISADDLKKAGAQIEEQEAEPQPKLV"
BASE COUNT         1034 a         1017 c          976 g         1036 t
ORIGIN      
        1 aatggactcc attacccgcc tcgcccgcgc ctacaacctg accgtcaacc cctctgggcg
       61 caccctctcc ggcggtatgg acccctccgc gttgtacccg cccaagcgtt tcttcggtgc
      121 ggctcgtaac gtggaagaag gcggctcgct aaccatcatc gcaacctgtc tcgttgacac
      181 cggttctcgt ctcgatgatg tggtgtacga agagttcaaa ggcaccggta acatggagtt
      241 gcacctctcg cgcaaattac aagagcgccg cacattcccc gctgtggatc tcgagcgctc
      301 gtccacccgc cgcgaagacc tgctgctcgg acccgacctg ctccagcgtg tttggctcct
      361 acgccgcatg ttcgtacaga tgacttcatc ccagcccgct ggcgccggta tggatccttc
      421 tgtggcgacc gaagccattt tgacgcgctt ggaaaaatca aagaacaaca tcgaattttt
      481 ggaaaatctt acaaaagacg cataacgaaa acaaaagttg aattggtaca aaatcgtaac
      541 acccattaaa aatgaaactt tttgaaagaa tcaaatccat cttcccggca cgcaagaaag
      601 ccaaagaaga agagcagcct caagctgttt cagcagtgaa tgaaccctca accagtccgc
      661 gtaaggaagt aaagaaagcg gatcgcttta ccatcgtcag ttggtctgtg acttcggtct
      721 tggttgtggc attgctcagt ggaacgctct attacaaaag cactcttccc gctactgtgg
      781 tgaccgtccc tgcggcgacg gaagaaccgg cggcagatgg cagtgtaccg gtagtggtca
      841 actcaggtac aggcggagga ggaagcgaag gtctcttccc taccattctc cgcaaattgc
      901 agatcaagac caacaagccg gaacgtccgc gctatgatcc agaactttat cgcgttgtgc
      961 gcggcgactc gatgtatcgc attgccgaag atttcaagat taagtctgaa accattttgt
     1021 acgtcaacga ccagttggaa gataacccac acagtttgcg tcccggcatg gagttaacca
     1081 ttcctccggc agatggtttg tattacacct ggaaagaagg cgatacgttt gaatctgtgg
     1141 cggaagaata taacgccaat gctgatgaca ttatcaactt cacaggtaat cagatcgacc
     1201 tgaccgaccc caaagtagaa gttggcaccc aaatttttat ccctggtggt tctcgtgagt
     1261 tggtgaactg ggcagcgcaa ttgcctacca atgcacgcgg agtgaacacc ggcacaggcg
     1321 gtggcaatgc cgccaatgtg tgtggcggtg gacctgtagc aagcggcttt ggttggcccg
     1381 ccgatgacca caccctctct ggaaatgctt acggacctgg tcaccttggc atcgatatca
     1441 gcgcccctga aggtgccaat gtgtatgccg ctggcaccgg tgttgtaacc atggcggctg
     1501 gcggttggaa ttacggttat ggcaacgtag tgcagatcga tcacggcaat ggttacgtaa
     1561 cggtttatgc ccacttaagc tccatcctcg tcagtcaatg tcaaaccgtg gggcaaggct
     1621 cggtgatcgg gctagcaggc aacacgggca actccttcgg cgcgcactta cactttgaaa
     1681 ttcgcgtcgg tggttcgaat atcaacccgt acgatattgt tcaataaaat agagacctga
     1741 cagtttcaaa gcctgtcagg tcttttaatt tccatgaacg aatcctctct caaaaaacga
     1801 cttgcaaaac taaacctcgg cggcttgcgc tattttgaat cgctgggctc tactaatgac
     1861 gaagctcttg catgggcagc tcgtggcgcg gatgatttct ctctcgtcgt ggcagatgaa
     1921 caaactcaag ggcgtgggcg gttagacagg aaatggttca cgcctaaagg cagtgcgatt
     1981 gcgatgagtc tcatcctacg ccctactgcg gtgatgcgct cgcatctctc gcggaccgtg
     2041 ggcttggcag ctctctcatt ggcagatgct tgtctcaagc tgggtctgtc tcccaagatc
     2101 aagtggccca atgatattct gctcgatagc aaaaagactg caggcatcct tgttgaaatg
     2161 gtgtgggctg gcgaagatgc cgattcattg gtaattggga tgggaattaa cgtccataag
     2221 tctgcagtgc cgccaagaga gacgctccaa ttccctgcaa ccagtttgga agatatgctg
     2281 ggaaacgctc cgaagcgaga agatatcatc tttcagattt tgagcaatgt gcaacgctgg
     2341 cgtgaacaga tcggcacaga gcggttcatt caggcgtggg aggaaaaatt agcctttcgc
     2401 ggagaggcaa ttcaaatcca cgctggaggc gcagagtctc atcagggtgt gttggatggt
     2461 ttggagtctg atggtagttt gcgcatctgt gatgaacata acaaatctgt tattgtgcgc
     2521 tttggggatg tgagtctgcg cccgactgca tgatatactt ttaaaaaata atcagaggtt
     2581 cccatgtttg atgatcttcg aaatgatgcc gcaaaacaat atgaagacga agtacaggca
     2641 gaatatcagc cagcttcagt cacttcatcg ggtgcgccgc gccaacgttc aaccaagttc
     2701 ctcggcatga catccataca gcgttttata cttgtgttct tattgatgac cgcaacctgt
     2761 gtgattggtt ttttgtgcct ttttgccttt ggaaaaatcg ctttctaagt cgcaacgcat
     2821 ttaacaatag ggtgtttagt atctgcatag agtgatagca tgcaaatact aaacaccctc
     2881 aattttttta caagcaaaaa tcttttcaaa agttataaac ggctgggatt ctggcaaacg
     2941 atcattcttt tttcgggggt tgctttcgtt tccgtaacag gcattatcgt ccctaccaac
     3001 atgctcttgg gcggaagtat cagaacaggc atggttatca acttcattgt ttgtacaact
     3061 ttcatgccgt accaccttca ccaaatcctt agcctcatgg tggagctaga cactttaaga
     3121 atgaacatat acgacaagtc gattcgggat gagttgacga aggcatataa ccgccgctac
     3181 ttttttgaag cgtctaaggc tatcgagaac aacgaagcct taattcccca aaatacatct
     3241 ctgctattaa ttgatattga tgatttcaaa ttgttaaatg acacctacgg gcacgatatt
     3301 ggcgaccttg cgttaaagac gcttacagaa gaaagctcgc gtctgttgcg tactacagat
     3361 gtctttgcac ggtatggtgg agatgaattt atctgcctgt tgcccaacac aaagcaatct
     3421 caagcctatg agatcgcaac ccgcctgctc gaaagcgtga acaaacatcc tttctacaaa
     3481 aaggatggga acatttttct ccggcttagt attgggattg ccacaacaac aagcgagacc
     3541 aaacttaatg acctaatttc tttagccgat aaagctttat ataaatcaaa acaaaacgga
     3601 aagaatactg tagaagcctt ataaaaaaag acctggtgat ttctcaccag gtctcttttt
     3661 atcgttatac caacttcggc tgtggttcag cttcttgctc ttcgatttga gcaccagcct
     3721 ttttcagatc atcggctgag atgcgtgcca ccgacgccac gctgtcacct tcttgtggct
     3781 tgatgagatg cacgccgcgg gttgcccgcc cagattgttt cacatcttta accttcaaac
     3841 gaatcgccac accattggca gtcataatgg tcaggtcgtc atctttgttg accacacgtg
     3901 ctgctgcaat cttgccgatc tcttggagcg ccttctggtc aatggtggaa ataccgcccg
     3961 tagcacgtcc tttgggtgta tattctttca aaggtgtctg cttcccaaat ccatttgtgg
     4021 tcacaaccag caatgcgcct tctttatcca ccacatccat gct
//
LOCUS       DAOQJE010000217         4059 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_13490_length_4059_cov_4.185814, whole genome shotgun sequence.
ACCESSION   DAOQJE010000217 DAOQJE010000000
VERSION     DAOQJE010000217.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 4059)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4059)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4059
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13490_length_4059_cov_4.185814"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1376
                     /gene="gguA"
                     /locus_tag="PLT08_07095"
     CDS             <1..1376
                     /gene="gguA"
                     /locus_tag="PLT08_07095"
                     /inference="COORDINATES: protein motif:HMM:NF040905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HNE04266.1"
                     /translation="VLSGVYPFGTYNGDILLDGKQQQFRHISDSEKAGIAIIYQELAL
                     IPEMTVYENIFLGHEIKKNNIVDWNETIKQAGEMLQKVKLDINSATKVKDLGIGKQQL
                     IEIAKALSKNVKLLILDEPTSSLNEKDSENLLQLLRDLKGQGVTSIMISHKLKEVISI
                     ADTVTVLRDGKTICTLSAAKGEVSENVLIKNMVGREINNIYPPKKHTPSQEVVLEVRN
                     WNARNPALGRDILKDVNIKVNRGEIVGLAGLIGSGRTELALSIFGNPAKYQVQGDLYV
                     QGEKRSFSHPEEAIRAGLSYVSEDRKGNGLILIQDVKQNITLANLKELTTRLVVNNNA
                     EIQVANKYKTDLNIKTPTVEQKVSNLSGGNQQKVSLGKWLFAKPNILILDEPTRGIDV
                     GAKYEIYTIMRDLVQKGLSIVMISSELPEVLGMSDRIYIVASGKIAGELSAQEATQEK
                     IMHLATN"
     gene            1389..2594
                     /gene="gguB"
                     /locus_tag="PLT08_07100"
     CDS             1389..2594
                     /gene="gguB"
                     /locus_tag="PLT08_07100"
                     /inference="COORDINATES: protein motif:HMM:NF040906.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HNE04267.1"
                     /translation="MNFFSTARKLLQQNIREYGMYIALFVIMVYFTIMTEGTFISSRN
                     IVNLVNQTGYIAVLAIGMTLVIIIRHIDLSVGFLAGFLGAIAAIAMADPKVVAAGMSL
                     PVYIVVPMTLGLGIVAGLITAFLVAQMKIPSFVASLTGWLIYRGAILVVTESTGTIII
                     NNEAFNAIGNGFIPDIPDIGILPGVHKLTLLLGIFAIVAMIFSELNERRKKQSYNFET
                     LPTEMFVIKLVFISGIIGTITWILAGYQGLSWTAVVVLVVTLVYHFIMSQTVLGRHIY
                     AVGGNPDAAELSGISVKRLVYIVFGSMGMLSALSGILFASRLQSATTTAGNLFELDAI
                     AAAYVGGVSAAGGVGKVLGSIIGALVMTSLTSGMNLMGIGISYQYMVRGAVLAAAVIF
                     DVATRNTGK"
     gene            2667..3839
                     /locus_tag="PLT08_07105"
     CDS             2667..3839
                     /locus_tag="PLT08_07105"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNE04268.1"
                     /translation="MNTSSRNLTSILLTAICFFAFFIFGTTDNLKGSTLPAMLAELNI
                     DYGTGGNIFFSEYVGFLIATLITGILADRFGLKIVFILAGIALVFGVGGYSSFSTPFL
                     LSTSLFIVGLGLGAFELGPNAVIVSLYHEQKGLYLNLMAVMHGLGSMIAPLVAGYLLS
                     MDVNWRTIYRFDLLLIAIFLVASLLVRFPKSEEKASLDFRAIPTVAFKHTLPFYYGAM
                     LFYVAAEIGLASWMVVYLQEARSLNETASNNYLAIFFGMLMLGRFFGSFFVKRVGYLR
                     SILFATLLATASIALGIFTNLVIFIPITGFFFSIIFPTLTAAVSDAEQENINTILGTL
                     FTFSGIGGMIGPWLVAWSSKLFGLQVGFSSIILLTGSTLVFAFILNQRSNTNGQRS"
     gene            3823..>4059
                     /locus_tag="PLT08_07110"
     CDS             3823..>4059
                     /locus_tag="PLT08_07110"
                     /inference="COORDINATES: protein motif:HMM:NF013567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HNE04269.1"
                     /translation="MDNVLNWGLLSTAKINRALIKPLRASKRTRLLGVASRSQSSAEA
                     YASEWKIPRAYGSYEAMLADPEIDVVYNSLPNPLH"
BASE COUNT         1038 a          979 c          902 g         1140 t
ORIGIN      
        1 aagttttaag tggtgtctat ccattcggaa cctacaatgg agacatcctc ctcgatggca
       61 aacagcagca atttcgtcat attagtgaca gcgaaaaagc tggtattgcg atcatttatc
      121 aggaacttgc tttgattcct gaaatgaccg tttatgaaaa catttttctt ggtcatgaaa
      181 ttaagaagaa caatatcgtt gattggaacg aaacgatcaa gcaagccggg gaaatgcttc
      241 aaaaagtaaa gttagatatc aattcggcaa caaaagtaaa agatctggga attggaaaac
      301 aacagctgat tgaaattgcc aaagcgttaa gcaaaaacgt caagttgttg attttggatg
      361 aaccaacatc ctcccttaat gaaaaagaca gtgaaaatct gctgcaatta ctccgcgacc
      421 taaaaggaca gggagtaacc agcatcatga ttagtcacaa actaaaagaa gtgatttcga
      481 tcgcagacac ggtgaccgtc ttacgcgatg ggaaaaccat ctgcacgtta agcgcagcca
      541 aaggtgaggt ttctgaaaat gttctgatca agaacatggt agggcgtgaa atcaataata
      601 tctatccgcc aaagaagcat actccctctc aagaagttgt tctcgaagtt cgaaactgga
      661 atgcacgaaa ccctgcactt ggcagggata ttctgaaaga tgtaaacatc aaagtaaatc
      721 gcggcgagat cgttgggctg gcagggctga ttgggtctgg gcgcacagag ttggcgctaa
      781 gcatctttgg caacccggca aagtatcagg tgcaaggtga tttgtacgtt caaggtgaga
      841 agcgcagttt cagccacccc gaagaagcga tccgcgcggg cttgtcgtat gtctcagagg
      901 accgaaaagg caacggcttg atcctcatcc aagatgtgaa gcaaaatatc acactggcaa
      961 atctcaagga actaacgact cgcctcgttg ttaacaacaa tgcagaaatt caggttgcca
     1021 ataaatacaa aacagacctg aacattaaga cccccactgt tgagcagaaa gtttcaaatc
     1081 tgagcggcgg gaatcaacaa aaagtaagcc taggcaaatg gctgttcgcc aagccgaata
     1141 tcctcatcct tgatgaaccc acacgcggca ttgatgtggg tgcaaagtac gagatctata
     1201 ccatcatgcg tgatctcgtc caaaaagggc tgagcattgt catgatttct tctgaacttc
     1261 ctgaagtgtt aggcatgagt gacagaattt acattgtcgc ttctggaaaa atagcaggtg
     1321 aactctctgc tcaggaagcc acgcaagaaa agatcatgca tttggcaacg aactaggaga
     1381 atacccttat gaatttcttc agtaccgcac gaaaactttt gcaacaaaac atccgcgaat
     1441 atggcatgta cattgcgctt ttcgtgatca tggtttactt caccatcatg acagaaggca
     1501 cgttcatttc atcgcgcaac atcgtcaact tggttaacca aacaggttac attgccgtgc
     1561 tcgccattgg catgaccttg gtcattatca ttcgccatat cgatctatca gtaggctttc
     1621 tagccggttt tctcggtgcc attgccgcaa tcgccatggc agaccctaaa gttgttgccg
     1681 caggaatgag cctgcctgtg tacatcgttg tgcccatgac acttggcttg ggcattgtcg
     1741 ccgggttgat caccgccttc cttgtcgctc aaatgaagat cccctctttc gttgcttcgc
     1801 taacaggttg gttgatctat cgtggtgcca ttttggtagt tacagaaagc accggtacta
     1861 tcatcatcaa taatgaagcc ttcaatgcca ttggtaatgg ctttatccca gatattccag
     1921 atattggcat tctccctggg gttcataagc tcacattact tctcggtatt tttgcgattg
     1981 ttgccatgat cttcagcgag cttaatgaac gcagaaagaa acagtcctac aactttgaaa
     2041 ccttgcctac cgaaatgttt gtcatcaagt tggtctttat ttcgggcatt atcggaacca
     2101 tcacttggat tttggctggg tatcaaggtc tctcgtggac agccgtagtt gtactcgttg
     2161 tcacccttgt ctatcatttc attatgtcgc aaactgtgct cggcaggcac atttatgcag
     2221 tcggcggtaa ccccgatgca gcggagttaa gcggcatcag cgtgaagcga ctggtctata
     2281 tcgtcttcgg ctcgatgggc atgctctcgg ctctttctgg aattttgttt gcctctcgcc
     2341 ttcaatctgc caccaccacc gcaggcaatc tcttcgagtt ggatgccatt gctgcggcgt
     2401 atgtgggtgg tgtctctgct gcaggcggtg tcggtaaagt gcttggctcc atcatcggtg
     2461 cgcttgtgat gacctcgctc accagcggca tgaatctgat gggcattggt atctcatatc
     2521 aatacatggt tcgcggagca gtgctcgccg cagctgtgat ctttgacgta gccacccgca
     2581 ataccggcaa ataattttcg cttcactcaa aatgagagac agggcttgtg tacaagccct
     2641 gtctcttttt gttctataat caccaaatga atacatcttc ccgaaacctt acttccattc
     2701 tcctcactgc catctgtttc tttgcttttt ttatctttgg caccaccgat aacctcaaag
     2761 ggtctacatt gccagccatg ttagcagagc taaacattga ctatggcaca ggtgggaata
     2821 tcttttttag tgaatatgtc ggcttcttaa ttgccacact cattaccggc atactggcag
     2881 accgctttgg tctcaaaatt gttttcattc ttgcggggat agctctcgtt ttcggagttg
     2941 gcggatacag ctcattcagt acaccgttcc tgttatcaac ctctctcttt atcgttgggc
     3001 ttgggctggg agcttttgaa cttggtccca acgcggttat tgtcagtttg tatcatgaac
     3061 aaaaaggctt atatcttaat ctcatggcgg tcatgcacgg acttggctcc atgatcgcgc
     3121 ctttagttgc aggatacctc ctaagtatgg atgttaactg gcggacgatc tatcgctttg
     3181 acttactgct catcgcaatt tttcttgttg ccagcctgtt agtacgcttt ccgaagtcag
     3241 aagaaaaagc ctcgttagat tttcgcgcca tcccaacggt tgcattcaaa cacacattgc
     3301 cgttttacta tggagccatg ctattttatg tcgcagccga aattggtttg gcatcctgga
     3361 tggtcgtcta tttacaagaa gcacgcagcc taaacgaaac agcaagcaat aattatctcg
     3421 ccatcttttt tggcatgctg atgttgggac gtttcttcgg cagtttcttt gtaaaacgcg
     3481 tcggctattt gcgctctatt ttatttgcca cacttcttgc cacggcaagc atcgcgcttg
     3541 gcatttttac caaccttgtg attttcattc caatcactgg ctttttcttt tcaatcatct
     3601 tccccactct caccgctgcc gtctctgatg ctgaacaaga aaacatcaat accattctcg
     3661 gtacgctatt taccttttct ggcatcggcg ggatgatcgg tccatggttg gtggcgtgga
     3721 gcagtaaact cttcggtttg caagtcggtt tttcatccat cattctattg acaggctcaa
     3781 ccctggtctt tgctttcatt ctcaaccaaa ggagtaacac aaatggacaa cgttcttaat
     3841 tggggattgc tctccaccgc aaaaattaac cgcgctttga ttaaacccct gcgcgcctcc
     3901 aaacggaccc gcttactggg cgtcgcttcg agaagtcaat cctccgccga agcctacgcc
     3961 agcgaatgga agatccctcg cgcctatggc agttacgaag ccatgctcgc ggaccctgag
     4021 atcgacgttg tctataactc tttgcccaat cccctccat
//
LOCUS       DAOQJE010000218         4053 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_13517_length_4053_cov_8.406453, whole genome shotgun sequence.
ACCESSION   DAOQJE010000218 DAOQJE010000000
VERSION     DAOQJE010000218.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 4053)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4053)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4053
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13517_length_4053_cov_8.406453"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..3851)
                     /locus_tag="PLT08_07115"
     CDS             complement(<1..3851)
                     /locus_tag="PLT08_07115"
                     /inference="COORDINATES: protein motif:HMM:NF024883.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HNE04270.1"
                     /translation="MNTPNPSDDAMKKRIDEMFSGQELPSYASLGEVNAMKARIRELE
                     EKLYQSTKPAVASLKLEEVNSVAEEKVTEKRPKSGILSLLTPPSFGNLAKDRIAKLQH
                     QILLGIFTTAILALIAVFLSWNGSGSLFGVGILSFEFVVLAFAFYFLRRGNLQVVSWI
                     LIGLIYSILISSLFVSGFTLATVIELALIVTMAGLLLHPWQVVIVSISAIVMNFVGPN
                     IGVPTTLPDNNLIFTGIILALEGALLTIASSTLENTFKEIDRSTKALVISNKNLQDIS
                     ANLEERVASRTYDLELATEVGKILSGKVDNLYGLLEDAVETIRARFNLYYTQVYITDP
                     SGRTITLRAGTGDVGKQLLKRGHHLLINPSSLNGRAASEKRTVIVSNTTDNPNFLPNP
                     LLPFTRSEMAVPLIAGDRVVGVLDMQSDQANFLTENNADAFEALAGQLAIAIQNAALF
                     EQVKLSRLELEAQTKKQSYENWQNFLNAIEREEKLGYVFDQSDVLPLIKAQGIEQTST
                     ALTAPIQVAGADIGNIQFIDNAERVWTPAEEDIVQNVVSQLSVHMDTLRLLAESDKYR
                     QDAELSTKRLTVEGWNEYLKSRQNTTEGFIYNDHKVLPLNGADIKTIGKEAVIPLQVQ
                     NQSIGEIAIETTEEFSDEELDILSNVSRQLSNHIENLRLFEQSNDSRKALEVTLQQLS
                     TNEARLSEALDIARLGNWEYDVEKDIFTFNDHFYAVFRTNVQEVGSYQMSSAEYAERF
                     VFPEDAPLVGIEIGKAMTTTERHFKQHLEHRIIFPDGSLGYISVNINVERDENGKVLR
                     WYGANQDITDRKMAERAVYEREEQFRNTLNNIPTAIVVTRMSDGNVLYANEALASMFG
                     APTQELIGKRSPDFYYNTNDRQRVVDIVRKDGYVKNNELLFKRITGEQFWALFNVYPL
                     KFFGENALMGSFYDLTERKEAEEAVRKAQEQYTLAVDGSNDGLWDWNIETNEVYFSPR
                     WKSMVGYEENELMNGFADFEALLHPDDHDRVLGVVNDYLTGKIDDYNVEFRFRHKNGS
                     YRWILARGKAMRNSDGAPYRMAGSHTEITERKEAEELIRTNEARLAEALEIARLGNWE
                     YDVENDIFTFNDRFYSVFRTNIQEVGSYQLSSAEYAQRFVHPEDLPIVGVEIEKALTS
                     KDRFYAASLEHRIVFPDATTGYISVNINVERDENGKITRYYGANQDITERKQSEELIA
                     KRAEQLSVLNRVMEVANTSQDKYYILQTATDEFRSLMQGFSAGVLLLDERGDMLTLVT
                     ESYADPSMPKLAGRSMPISVNP"
     gene            complement(3865..>4053)
                     /locus_tag="PLT08_07120"
     CDS             complement(3865..>4053)
                     /locus_tag="PLT08_07120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE04271.1"
                     /translation="YMEAQSQAERENIINEINRKIQNTSSVEQALQVAIRELGSTLGA
                     KDSRIVLHLPDSVTKESR"
BASE COUNT         1041 a          884 c          921 g         1207 t
ORIGIN      
        1 gggttaaccg agatcggcat ggagcgccct gctaattttg gcatggaggg gtccgcatag
       61 ctttcagtga ccagggtcaa catatcgccc ctttcatcca ataagagtac accggcgctg
      121 aagccttgca ttaagctgcg gaattcatct gtagcagttt gcaagatgta atacttatct
      181 tgtgaggtgt tcgccacttc catcacacgg ttaagcacag atagttgttc cgcacgtttt
      241 gcaatgagct cttcagattg tttacgctcg gtgatatctt gattggctcc atagtaacga
      301 gtgatttttc cgttctcatc gcgttctacg ttaatgttga cagaaatata gccagttgta
      361 gcatctggga aaacaatgcg atgttcaagt gaggcagcat aaaaacggtc tttggaggta
      421 agagcctttt cgatttctac accaacaatg ggtagatcct caggatgaac aaatctttgg
      481 gcatattctg ctgatgacag ttggtagctt ccaacttctt gtatatttgt gcggaagact
      541 gaatagaagc ggtcgttgaa agtaaagata tcgttttcaa catcgtattc ccagttacca
      601 aggcgggcaa tttcaagcgc ttcagccagg cgggcttcgt tggtacggat gagctcttct
      661 gcctctttgc gttcggtaat ctctgtatga gaccctgcca tacgataggg agctccatcg
      721 ctgttacgca tggctttacc tctagcgagg atccaacgat acgacccgtt tttatggcgg
      781 aagcggaatt cgacgttgta gtcatcaatt tttccagtca ggtagtcgtt tacaacacca
      841 agcacacggt catgatcatc ggggtgcaga agggcttcga aatcagcaaa gccattcatt
      901 aattcatttt cttcataccc gaccatggac ttccagcgtg gtgagaagta cacctcgttt
      961 gtctcaatat tccagtccca taagccatcg ttagaaccat ctaccgcaag ggtgtactgt
     1021 tcctgcgctt tgcggacagc ttcttctgct tctttacgct cagtaaggtc gtagaacgaa
     1081 cccataagcg cattttcacc aaagaatttc aatgggtata cgttaaagag cgcccagaac
     1141 tgttctcccg tgatacgctt gaataggagt tcattgttct ttacataacc gtcttttcgc
     1201 acaatgtcca caactcgttg acggtcattt gtattgtaat aaaagtctgg cgatcgttta
     1261 ccgattagtt cttgagtagg tgcgccaaac atgcttgcta atgcttcatt tgcatatagt
     1321 acattcccat cagacatgcg ggtaaccaca attgcagttg gaatattgtt gagcgtattt
     1381 ctgaattgtt cttcacgctc atagactgct cgttctgcca ttttgcggtc ggtgatatcc
     1441 tggttggcac cataccagcg aagaaccttt ccattttcgt cgcgttcaac gttgatattg
     1501 accgagatat acccaaggct gccatctggg aaaataatgc gatgttccag atgctgtttg
     1561 aagtggcgct ctgtggtggt catcgctttt ccgatttcga taccaaccaa cggagcatct
     1621 tcagggaata caaaacgctc ggcatattca gcagaagaca tctgataact tccgacttct
     1681 tgtacatttg tgcggaatac agcatagaaa tggtcgttga atgtaaagat atctttttca
     1741 acgtcatatt cccagttacc aaggcgggcg atatcgagcg cttcagaaag acgggcttcg
     1801 ttggtgctta attgttgtag tgtaacttcc aacgcttttc gactgtcatt gctttgttcg
     1861 aacaatctta agttttcaat atgattgctc aactggcgtg aaacattgga aaggatatcc
     1921 agttcttcat cgctaaactc ttcggtcgtt tcaattgcaa tttcgccaat gctttgattt
     1981 tgcacctgga gcggaataac cgcctctttg ccgatggttt tgatgtctgc gccgttgaga
     2041 ggaagtactt tgtgatcgtt ataaatgaaa ccttctgtcg tgttttgacg tgatttaagg
     2101 tattcattcc agccttctac ggttagacgt ttcgtactta actctgcatc ttgtcgatat
     2161 ttatcagact ctgctaatag cctgagggta tccatgtgaa ccgaaagttg ggaaactaca
     2221 ttttgtacaa tatcctcttc ggcaggagtc cagacccgtt cagcattgtc tatgaattgg
     2281 atattgccaa tgtctgcacc agccacttga ataggggcag ttagagccgt tgaggtttgt
     2341 tctatacctt gtgcttttat caacggtaag acatcacttt ggtcaaagac atacccaagc
     2401 ttttcttccc gttcgatcgc gttcaggaag ttttgccagt tttcatagga ttgtttcttt
     2461 gtttgggctt cgagttccaa acgagatagt ttgacctgtt caaagagagc tgcattttga
     2521 atggcaattg ccaattgccc agccaaggct tcaaaggcat cggcattgtt ttcggtaagg
     2581 aaattggctt gatcgctttg catatccaaa acgcctacaa cgcgatcacc tgcaatcaaa
     2641 ggaacggcca tttcagaccg ggtgaagggg agtaaggggt tggggaggaa gttgggatta
     2701 tcggttgtgt tcgaaacgat aacggtccgt ttttcagaag cggcacgtcc attcaatgag
     2761 ctagggttga tcaaaaggtg gtgaccacgc ttaagcaatt gcttgcctac atctccagtt
     2821 cctgcacgga gggtgatggt gcgcccagaa ggatctgtaa tataaacttg ggtatagtac
     2881 aaattgaaac gagcgcggat tgtttccacc gcatcttcca ataagccata taagttatct
     2941 accttgccag ataggatctt accgacttcc gtagctaatt caaggtcata agtacggctt
     3001 gctacgcgtt cttcaaggtt ggcagaaata tcttgtaagt ttttattact gataactaac
     3061 gcttttgtac ttctgtcaat ttctttgaat gtgttttcca gggtgctaga tgcaattgtt
     3121 aataaagcac cttccaacgc tagaatgatg ccggtgaaga taagattatt atctggtaat
     3181 gtggttggga caccgatatt cggacctaca aagttcatca caatagcact gatagacaca
     3241 ataaccactt gccatggatg aagaagcaaa cctgccatgg taacgatcag cgccagctcg
     3301 attacggttg ccagtgtaaa cccgctgaca aagagactgg aaattaagat ggaatagata
     3361 agaccaatta atatccaact caccacttga agattgccgc ggcgtaagaa ataaaaggca
     3421 aaagctagaa caacaaattc gaaggacaga atgcccaccc cgaacaaaga acctgagccg
     3481 ttccagctta gaaaaacagc gatgagcgcc aagattgctg ttgtaaagat acccagcaaa
     3541 atttggtgtt gtaatttggc aatacggtct tttgccagat taccaaaact tggcggggtt
     3601 agtaggctta agatgccaga ctttggacgt ttttcagtca ctttttcttc tgccacagaa
     3661 ttaacttctt ctaacttcag tgaagctaca gcaggctttg tggattggta cagtttttct
     3721 tccaattcac gaatgcgagc tttcatggca tttacttccc ctaggctggc ataggaaggt
     3781 aactcttgcc cagagaacat ttcgtcaata cgttttttca tggcgtcatc agatggattt
     3841 ggagtattca taaaggttct ccttctaacg gctttctttc gtaacagaat caggcaggtg
     3901 caacactatc cgcgaatctt ttgctcccaa tgtggaacct agttcgcgga tggctacttg
     3961 caaggcttgc tctaccgaag aagtattttg aatctttcgg ttgatttcgt tgatgatgtt
     4021 ttcgcgctct gcctggcttt gggcttccat ata
//
LOCUS       DAOQJE010000219         4052 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_13525_length_4052_cov_6.924443, whole genome shotgun sequence.
ACCESSION   DAOQJE010000219 DAOQJE010000000
VERSION     DAOQJE010000219.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 4052)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4052)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4052
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13525_length_4052_cov_6.924443"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(174..>1304)
                     /gene="hutU"
                     /locus_tag="PLT08_07125"
     CDS             complement(174..>1304)
                     /gene="hutU"
                     /locus_tag="PLT08_07125"
                     /EC_number="4.2.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016153 - urocanate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006548 - histidine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urocanate hydratase"
                     /protein_id="HNE04272.1"
                     /translation="GAQPLSITMNEGVGLIVEVDPERAERRRAIGYVDMVVDNLEEAM
                     TLVEENVKNGTPKSIGLIGNAADVYTELSQRGIIPDVVTDQTSAHEALFYVPSGLSIT
                     AANQLRESDPEKYKAMSMDSMAKHVQAMLAFQRAGAEVFDYGNNLRQQAYNHGVSDAF
                     EFPGFVPAYIRPLFCEGKGPFRWVALSGDKEDIYVTDQAIMELFPEDEHLHRWLKMAK
                     EKVPFQGLPSRICWLGYGERVKAGLMFNELVATGKVKAPIVIGRDHLDSGSVASPNRE
                     TEAMKDGSDAISDWAILNAMINAVGGATWVSFHHGGGVGMGYSQHAGQVIVADGTPEA
                     AKRLERVLTTDPGMGVVRHADAGYDIAVEAAKRHGIKMPMLK"
     assembly_gap    1305..1404
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1405..1613)
                     /locus_tag="PLT08_07130"
     CDS             complement(<1405..1613)
                     /locus_tag="PLT08_07130"
                     /EC_number="4.2.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561536.1"
                     /note="catalyzes the formation of
                     4-imidazolone-5-propanoate from urocanate during histidine
                     metabolism; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urocanate hydratase"
                     /protein_id="HNE04273.1"
                     /translation="MSTPRVIRAPRGTELTCKNWLSEAAYRMIQNNLDPEVAERPEDL
                     VVYGGRGKAARTWEAYDAILESLKNL"
     gene            complement(1626..2477)
                     /locus_tag="PLT08_07135"
     CDS             complement(1626..2477)
                     /locus_tag="PLT08_07135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE04274.1"
                     /translation="MKQHRGFLMAVLLMLTALACVVPGLQPASAIPPTPDTRLDRMVA
                     ETVAAALALTQQVPTATNTSTPTPTSTPTSTPLPVEPTQAEESVLDKNTDGTSSFIDL
                     SGKYQFTVPTQWLALRINSPEYDEAVLLPEAANPAIQNQLSVIQKQDPNVFRLFLLDI
                     NEEHIDSGFVTNVNLVWDPQMEISVATDSDLKGLAATLPTSLKDAEVTGVEIKTTTGG
                     LSYGVITATTPLMTQEGARLNVYQKLVFFDLPTGTLSVTLSTTEKWQSTVEPFFDEFV
                     TSFLILE"
     gene            complement(2568..2981)
                     /locus_tag="PLT08_07140"
     CDS             complement(2568..2981)
                     /locus_tag="PLT08_07140"
                     /inference="COORDINATES: protein motif:HMM:TIGR00738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HNE04275.1"
                     /translation="MQITRQADYAVRAVLHLARNGETRTATSLIAEEQKIPPSFLAKI
                     ISQLSIAGLLHTSRGARGGVTLAREPKDITLLEVIEAIDGPIQLNECVGDTGTCSFDD
                     NCPLRPVWCEAQEQLVGRLKGTNFADMIAKGQTAA"
     gene            complement(3169..4011)
                     /locus_tag="PLT08_07145"
     CDS             complement(3169..4011)
                     /locus_tag="PLT08_07145"
                     /inference="COORDINATES: protein motif:HMM:NF028425.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toast rack family protein"
                     /protein_id="HNE04276.1"
                     /translation="MYRKLIPFLSILALTALACGISVPKPPTPQPEVTEDIKADYPDA
                     DEVNLALRFGAGEMTLNPGADVLVDGTATYNYDELKPEVNTDGGDVEVRVGDGNLNIF
                     PNLNNLKNVWDLKLGDQPMNLSIESGAYDGTFELGGLSLTRLDIKDGAANVELAFSEP
                     NPVEMSTFTYSTGASDVKITGLANANFSFFDFSSGAGDYTLDFSGDLQRDASVKIETG
                     LSNFIIIVPEGVDAVVTVQGGLSNVNAGPGWERSGSDYIQKGEGPTITFVIEMGAGNL
                     TLAR"
BASE COUNT          994 a          986 c          996 g          976 t
ORIGIN      
        1 aaacccgtag gcgtgggaac aggcggcaga gagctacccc aaatattggg gagcgtgcca
       61 caacctgcga taagaaaagc aaggagaaga agagtaaggc gtttcagaag atttctccaa
      121 tgataaaagg cgaccctaaa tccagatggg tcgccccgat gattccgtac agattatttc
      181 aacattggca ttttgatgcc atgacgtttc gcagcttcca ctgcaatatc atacccagca
      241 tcggcatggc gcaccacacc cataccgggg tcggtggtga ggacacgttc caagcgtttg
      301 gcagcttctg gtgtgccatc cgcaacaatg acctgaccag catgttgtga atatcccatg
      361 cccacgccac caccgtgatg gaaggaaacc caagtagcgc cacccacagc gttgatcatg
      421 gcattgagga tcgcccagtc cgaaatcgcg tctgaaccgt ctttcatcgc ttcggtctcg
      481 cggtttggcg aagcgaccga acccgaatcc aaatgatcac gtccgatcac gatcggggct
      541 tttactttgc cggtcgcaac caattcgtta aacatcaaac cggctttcac acgctcgcca
      601 tacccaagcc aacaaatacg cgaaggcaaa ccttggaacg gcactttttc ttttgccatc
      661 ttcaaccaac gatgcaaatg ttcgtcttca gggaagagtt ccataatggc ttggtcagtc
      721 acataaatat cttctttgtc gccagaaagc gccacccacc gaaacggacc tttgccttca
      781 caaaacagcg ggcggatgta agcaggcaca aagccgggga attcaaaggc atcgctcacg
      841 ccatggttat aagcttgctg gcgtaaattg ttgccataat caaatacttc cgcaccagca
      901 cgctggaaag caagcatcgc ttggacatgt tttgccatcg aatccatcga catggctttg
      961 tatttttccg ggtcagactc tcgcaattga tttgccgctg taatgctcaa ccccgatggg
     1021 acatagaaca acgcctcatg cgctgacgtt tggtctgtca ccacatccgg aatgattcca
     1081 cgctgtgaga gttcggtgta cacatccgca gcgttaccga ttagcccaat agacttgggt
     1141 gtgccattct ttacattttc ttccacgagg gtcatggctt cttcgaggtt atccacgacc
     1201 atgtccacat agccaatggc gcgacgacgc tctgcacgct cggggtccac ttcaacaatg
     1261 agaccgacac cttcattcat ggtaatggaa agcggttgag cgccnnnnnn nnnnnnnnnn
     1321 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1381 nnnnnnnnnn nnnnnnnnnn nnnnagattt ttcagcgatt ccaaaatcgc gtcatacgct
     1441 tcccaagtcc gcgcagcctt cccccgccca ccgtagacga caaggtcttc tggcctttct
     1501 gccacttcgg ggtcgaggtt gttctggatc atacgatagg cagcttcgct gagccagttc
     1561 ttacaagtca gttcggtgcc gcgtggcgcg cggataacac ggggtgtgga catgtttagt
     1621 ctccgttatt ccaatatgag gaaagaggta acgaattcat caaagaatgg ttcaactgtg
     1681 ctttgccact tttcagtggt ggaaagagtc acagataagg ttccggtagg caaatcaaaa
     1741 aataccaatt tttgatacac attcaaccgg gcgccttctt gggtcatgag aggtgtggtg
     1801 gcagtgataa cgccatacga taaaccgccg gttgtagttt tgatctcaac accggtcact
     1861 tcggcatctt tgagagacgt gggcagagta gccgccaagc ctttgaggtc tgaatcggtg
     1921 gcgaccgaaa tttccatttg cgggtcccat accaaattga cgttggtgac aaagccgctg
     1981 tcgatatgtt cttcattgat atcaagcaag aaaagacgga agacattcgg gtcttgtttt
     2041 tggatgaccg aaagttgatt ttgaattgcg gggtttgccg cttcgggcaa caacacagct
     2101 tcatcatatt ccggcgagtt gatgcgcaac gcaagccact gtgtgggaac cgtaaattga
     2161 tacttaccgc tcaagtcaat gaaagagctg gtgccatcag tgttcttatc caagacactt
     2221 tcttcggctt gggttggctc aacaggcagc ggcgtgcttg tgggtgtgct ggttggggtc
     2281 ggggttgaag tattggtggc tgtaggaacc tgttgcgtta acgcgagagc ggcagcaacg
     2341 gtctcagcga ccatgcggtc aagtcgtgta tcaggtgtcg gcggaatcgc agaggcgggt
     2401 tgtaatcccg gcacgacaca tgccaaagca gtcagcatga gtaagactgc catcaaaaat
     2461 ccacggtgtt gtttcatagg tttatccttg caaatggaat tacccatagg gtatataaaa
     2521 aaaagcctcc cggctattat agccgagagg ctttgatttc tttgacctta cgccgcagtt
     2581 tgacctttgg cgatcatatc tgcaaagttg gtgcctttca aacgaccaac caactgttct
     2641 tgtgcttcgc accataccgg gcgcagcgga cagttatcgt caaaggagca agttccggta
     2701 tcgcctacac attcattgag ctgaatggga ccgtcgatag cttcgatgac ttcaagcaga
     2761 gtaatatctt tcggttcacg cgcaagggtt acaccgccgc gcgctccgcg ggaggtgtgc
     2821 aacaacccgg caatcgaaag ttgagaaatg atcttcgcaa ggaaggaggg tggaattttt
     2881 tgttcttcag cgattaagct ggtggcagtg cgggtttcgc cattgcgagc aagatggagc
     2941 acagcgcgta cagcataatc ggcttgtcgg gttatttgca taaggacctc acttaatata
     3001 ggtaaaactt acccacaatc acgattttat tgtgaacggc gttacaaggc aagtgataga
     3061 ggtcactgat gaattatgac ctttcgcgct taattagaaa gatggttttt aaacgaaaaa
     3121 cggattactt gtaaggtaat ccattttttc aacttgtaaa attctttcct aacgtgccaa
     3181 ggtcagattc cctgccccca tctcgatgac aaaggtgatg gttggacctt ctcctttttg
     3241 gatgtaatcg cttccgcttc tctcccagcc gggacctgca ttcacattcg acagcccacc
     3301 ctgcactgtg accactgcgt cgaccccttc gggcacaatg atgatgaaat tgcttaagcc
     3361 ggtctcgatc ttgaccgaag catcgcgctg caaatcaccg gagaaatcaa gagtgtagtc
     3421 acctgcgccg gaagaaaagt cgaagaagct aaagttggca tttgccagcc cagtgatctt
     3481 cacatccgaa gcgccagtgc tataggtaaa ggtgctcatc tcaacgggat ttggttcaga
     3541 gaaagcgagt tccacgtttg cagcgccgtc tttgatatcc aagcgcgtga gggagagccc
     3601 gcctaattcg aaggtgccat cataagcgcc agactcaata gacaggttca tgggctgatc
     3661 gccgagtttc aggtcccata cattcttgag gttattgaga ttcgggaaga tgttgagatt
     3721 cccatctccc acgcggactt ccacatcgcc gccatcggtg ttgacttcag gtttgagttc
     3781 gtcgtagtta tacgtagcag tgccatctac aagcacatca gcgccggggt tgagagtcat
     3841 ttcgcctgcg ccaaaacgca atgccaagtt gacctcatcg gcatctggat agtcggcttt
     3901 aatatcttca gtcacttcgg gttgaggtgt aggtggcttg ggtacactaa ttccacacgc
     3961 caacgccgtc agcgcaagaa ttgagagaaa gggtataagt ttgcggtaca taaagaaaaa
     4021 cctccttagg ttattactaa gaaggtatac gg
//
LOCUS       DAOQJE010000220         4034 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_13594_length_4034_cov_6.497864, whole genome shotgun sequence.
ACCESSION   DAOQJE010000220 DAOQJE010000000
VERSION     DAOQJE010000220.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 4034)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4034)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4034
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13594_length_4034_cov_6.497864"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..206
                     /locus_tag="PLT08_07150"
     CDS             <1..206
                     /locus_tag="PLT08_07150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase"
                     /protein_id="HNE04277.1"
                     /translation="KTRRVVDDGISVPDSTKKSGQRLVGDVNFAEAKEVAGFITPVPG
                     GVGPMTIAMLMANTFRAAEIQDK"
     gene            439..795
                     /locus_tag="PLT08_07155"
     CDS             439..795
                     /locus_tag="PLT08_07155"
                     /inference="COORDINATES: protein motif:HMM:NF015513.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HNE04278.1"
                     /translation="MVEPGSHTKKFQKELNAGTSSLVLLSVMSRSDEPMYGYQIAKML
                     EESAPDIPMMKQGTLYPVLRSLEENGLLESTVEPSVSGPPRRYYKITKDGQAALAEWT
                     ELWKQTKKFVDSILKG"
     gene            800..1687
                     /locus_tag="PLT08_07160"
     CDS             800..1687
                     /locus_tag="PLT08_07160"
                     /inference="COORDINATES: protein motif:HMM:NF025168.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor domain-containing protein"
                     /protein_id="HNE04279.1"
                     /translation="MFKTIEDYLDALKNEMKDADPALVQDAQSDAREHLTMALSAAKE
                     KQPEISDADALTSIIDGYGSPEETASAYKEVERRTSPALTQTVKPQSFLGRFFGVYLD
                     PRAWGALFYMLIAFVTGIFYFTWAVTGVSLSLSLMILIFGLPVALLFLISIRGLALLE
                     GRLVEALLGIRMPRRPLFTHQGMKWLERLKALLVDRQTWMMLLYMVLQFVLGIVYFVV
                     IVMVLSFSLAFIALPILQETLHMGAVVINDTRFFMPSWSYPLFVVGGFLLWTIFMNIV
                     RGVGQLHGRLAKWILVGES"
     gene            complement(1744..>4034)
                     /locus_tag="PLT08_07165"
     CDS             complement(1744..>4034)
                     /locus_tag="PLT08_07165"
                     /inference="COORDINATES: protein motif:HMM:NF021507.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HNE04280.1"
                     /translation="PESIRLTNNEETLMVNVIGPDISFYQDDPQTPQGVNFIKMRESA
                     YYVIVRAGQNLWGDRDFKVNWRESKAAGLPRGSYWFYDSRIDPKQQAEKWISMFDGDF
                     GELPLWGDFEDTYNGPYKGWKNWYNFLERLKQLLPSDKEVGVYTGYYYWRENTVAVGI
                     PNASLNYFKQYPLWIANYGTSAPLVPEPWDTWTLWQFTDNGDGPAYGVESLNIDLNYF
                     NGDLEALKTRFKLGDLNFPPAPTPSNYYRVTVSSLKVRDAAGLNSTQIGSITLNEVVE
                     KLEANSDGTWLKIRKKDGSLTGWSFAAYLQNLSSTDSSTDNSDGSNSGGSGSGSGNSG
                     GGTSGGDTGTNNPPDEEDTNWYRVTASSLKIRSGPGLEYDAIGTFTLGELVEKLEATT
                     NNDWLKVRSKDGTKTGWCSGEYLISVHNPSDEQASGTPVDEHADKNWYRVNTVTLNLR
                     ETAELNGKILSTLTKNDVIPGLDESNNKWLKTEKLDGTTGWVSRDYLVQVSTASRPSS
                     ISQKLFAGVTYLRKDLTSPRQIVVHVFAVDLKELKLEFLVTPSTDNTDVLCTRVTSKF
                     LEEFKLHAAINGGYFSYIETSSSSTSLCPNGGDPVRISDYAASRGKIYSPKKTAQPVV
                     HISRKNVVSFNKAPGAVFNAVAGDRMVVVDGKVVKNLAAQVPNPRTAVGLNKNGRWLT
                     MMVVDGRQPGFSEGVTFPELAELLISYGVHIGANMDGGGSSTMVIRGADGKARVLNSP
                     IEMNQPGKERPVGNHLGLFIKP"
BASE COUNT         1062 a          910 c          931 g         1131 t
ORIGIN      
        1 aaaaaacaag aagagtagtt gatgatggaa tttctgtgcc ggattcgacc aagaagagcg
       61 gtcaacgcct tgtaggtgat gtcaactttg cagaggcgaa agaagtcgct gggtttatta
      121 ccccggtacc tggtggggta ggaccgatga ctattgccat gttgatggca aacacattca
      181 gagcggctga aatacaagat aaataaagta attcagaaca ggaattttta tcaatcccgt
      241 cgcgaggcgg gatttgtttt gtgtgaatac agagaacaaa atgaaaaaat actaaattat
      301 gaaaatcgct gatttttata gcactaaaga gctagccaaa agcggctctt ttttatttac
      361 acccttgact taatatcaaa ttgatactat tataagattg ttactagtaa gaatttgata
      421 ctattgaaag gagtaggaat ggttgaacct ggatcgcata ccaaaaaatt ccaaaaggaa
      481 ctgaatgcag gcacatcttc gttggttctt ttgagtgtta tgagccgcag tgatgaaccc
      541 atgtatggct atcagattgc aaaaatgctg gaggaaagtg ccccagacat cccaatgatg
      601 aagcagggga cgttataccc cgtacttcga tcgttggaag aaaatggctt gttggaaagt
      661 acagtggagc cgtcggtctc cggtccgcca cgccgctatt acaaaatcac caaagatggg
      721 caagccgcgt tggcagaatg gacagaatta tggaaacaaa cgaagaaatt cgtagactcg
      781 atcttgaaag gatgacatca tgtttaagac gattgaagat tatttagatg ctttaaaaaa
      841 tgagatgaaa gatgccgatc cggctttggt tcaagatgcg cagtctgatg ctcgtgaaca
      901 cttaacgatg gcattaagtg ccgcaaaaga aaaacagcct gaaatttctg atgccgatgc
      961 cttgacctcc attattgatg gatacggttc accggaagaa actgcctctg catacaaaga
     1021 agtggagcgc cgcacgtccc ccgcattgac acaaacagtc aagccgcaat cttttttagg
     1081 tcgttttttc ggcgtttacc ttgacccacg tgcttggggc gcattgtttt acatgttgat
     1141 tgcatttgta accggcattt tctatttcac gtgggctgtt acgggtgtat cactttctct
     1201 ttccttgatg attttgatct ttggcttacc ggttgctttg ctttttctaa tttctattcg
     1261 agggctagca ttgttagaag gtaggttggt agaagctttg cttggcattc gtatgccgcg
     1321 ccgtcctttg tttacacatc aaggcatgaa atggcttgaa cgcctcaaag cattgctcgt
     1381 tgaccgccaa acctggatga tgttgcttta tatggttttg cagttcgtgc tgggcattgt
     1441 gtactttgtt gtgatcgtaa tggtactgag tttttctcta gcgtttattg ctttaccaat
     1501 tttgcaagaa actcttcaca tgggtgcggt ggtcataaac gatacaagat ttttcatgcc
     1561 atcttggtcg tacccattat tcgtggtggg cggtttcctc ttgtggacaa tttttatgaa
     1621 tatcgttcgc ggagtgggac aattgcatgg aagactcgcc aagtggattt tggtgggcga
     1681 atcctgatat aaggaaataa aaacagtccg gctcaaaacc ggactgtttt tatttcctgt
     1741 ttcttatggc ttgataaaca gccctaagtg attcccaacc gggcgttcct tgccaggttg
     1801 attcatttca atgggagagt tcagcacgcg cgctttgccg tctgcgccgc gaatgaccat
     1861 ggtggaggac ccgccaccgt ccatgtttgc gccgatgtgg actccatatg aaattaacaa
     1921 ctcagctaac tcagggaagg tcacgccttc gctaaaccca ggctgacgtc catctacaac
     1981 catcatcgtg agccagcgac cgttcttatt caacccaacc gccgtgcgtg ggtttggcac
     2041 ttgagcagcg aggtttttca caactttgcc gtctacaact accatacggt cacctgccac
     2101 ggcattaaag acagcacccg gcgccttgtt aaaactaact acatttttac ggctgatatg
     2161 aacaaccggt tgagccgtct ttttcgggga atagatcttg ccacgcgaag ccgcataatc
     2221 gctaattcgc accgggtcgc cgccattggg acataaagat gtagatgaac tggacgtctc
     2281 aatgtaactg aaatatccgc cgtttatagc agcgtgcaac ttgaactctt ccaaaaattt
     2341 agaggttacg cgtgtgcaca atacatctgt attatctgtc gatggggtga caaggaattc
     2401 aagctttagt tccttgaggt ctactgcaaa gacatgtaca acgatctgcc gtggagaagt
     2461 gaggtctttt cggagatatg tgaccccagc gaataacttt tgagaaatag aactggggcg
     2521 gctggcagtg ctgacctgta ccagataatc acgagatacc cagcccgtcg ttccatccaa
     2581 cttttcagtt ttgagccatt tgttgtttga ctcatccaag cccggaataa catcattctt
     2641 tgtaagggtg ctaagaattt tcccattaag ttcagcagtt tctcgcaggt ttagggttac
     2701 ggtattaacc cgataccagt tcttgtctgc atgttcatca acaggggttc cagaggcttg
     2761 ttcgtctgat ggattatgaa cgctaataag atactcacca gaacaccagc cggttttggt
     2821 cccatcttta ctgcggactt taagccagtc attgtttgta gtagcttcta gcttttctac
     2881 aagttcgccc aacgtaaagg tgccgatggc atcatattcc aaaccgggac ctgagcggat
     2941 cttcaacgaa gacgccgtca cacgatacca attggtatct tcctcatctg gcggattgtt
     3001 tgtaccggta tcgcctccgc tggtgccgcc accgctattt ccagacccag agccgctgcc
     3061 gccagaattc gaaccgtcag aattatcggt agaagaatcg gtgctgctca aattttgaag
     3121 ataggcggca aaactccacc ccgtgaggct gccatccttt ttacggatct taagccaggt
     3181 tccatctgaa ttggcttcga gcttttctac tacttcgttt agggtaatgc tcccgatctg
     3241 agtggaattc aaacccgccg catcgcgaac tttgagtgaa gaaacggtca cgcgataata
     3301 attgctgggg gtaggggctg gagggaagtt aagatctccc agtttgaagc gggttttgag
     3361 agcttccaga tcaccattga aatagttcaa atcaatattg aggctttcaa ccccatacgc
     3421 aggaccatct ccattatccg tgaattgcca gagcgtccat gtatcccaag gctcaggaac
     3481 gagcggagca cttgtcccat aattggcgat ccataatgga tattgtttga aataattcag
     3541 cgaagcattt ggaattccga ccgcaactgt attttcgcgc cagtagtaat acccagtgta
     3601 aacaccaact tctttatcag aagggagcaa ctgtttcaac ctctctaaaa agttgtacca
     3661 gtttttccaa cctttataag gaccattgta cgtatcttca aaatcgcccc aaagtggcaa
     3721 ttcgccaaaa tcgccgtcaa acatcgagat ccatttttca gcttgctgtt tcgggtcaat
     3781 gcggctgtcg taaaaccaat aagaaccgcg tggcaaaccg gctgcttttg actcacgcca
     3841 attgactttg aaatccctat cgccccataa attttgccct gcacgcacga tcacataata
     3901 agcgctttca cgcatcttaa taaaattgac gccttgcgga gtctgtggat cgtcctgata
     3961 aaaactaata tcgggtccga tgacatttac catgagagtc tcctcgttgt tcgttagccg
     4021 tatgctttca ggtt
//
LOCUS       DAOQJE010000221         4033 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_13599_length_4033_cov_5.299899, whole genome shotgun sequence.
ACCESSION   DAOQJE010000221 DAOQJE010000000
VERSION     DAOQJE010000221.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 4033)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4033)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4033
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13599_length_4033_cov_5.299899"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            148..969
                     /locus_tag="PLT08_07170"
     CDS             148..969
                     /locus_tag="PLT08_07170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE04281.1"
                     /translation="MNLRYLFLRLLRHFMPEGLARFLLKRRWIIKPGLESTDPFAASA
                     RYVNTLAAQGVSITGKRVLVFGYGGRFAVGVDLLKRGAAHVVLCDHFVLLDTERNREL
                     LSAYGQYLKVEGDEVQPRSEFITLLHGDIREEAIQKQISQVDVVLSTSVFEHLDDVPG
                     ITKALAKLTASQGIHLHFVDLRDHFFKYPFEMLKYSESVWKHFLNPTSNLNRYRWNDY
                     KQVFEENFGQVSFIVIERLVEEFSKVKSKIRPEFRTGDENIDAVTLIHVIAKQPK"
     gene            982..2631
                     /locus_tag="PLT08_07175"
     CDS             982..2631
                     /locus_tag="PLT08_07175"
                     /inference="COORDINATES: protein motif:HMM:NF024629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000030 - mannosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006493 - protein O-linked glycosylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid carrier-dependent
                     glycosyltransferase"
                     /protein_id="HNE04282.1"
                     /translation="MHNKLNTFFTRYEFLLPLFVFAIFLTASLPGNSWGAPALWNPDE
                     LVWRVDMALHGNMRFDVTEPDYNYPSLPKYVMYAIGYVTYGLGRSNYAFIVAARSFSA
                     FLGALAGVLIYYLARLIGANKRTATLAGLFYVISGIAAENGRFAHNDLYLQFFTILSV
                     FFVIKYQHTTKNNWLYLSFFSVGLAASCKYTGGSLIILPIIVFIAANWSDIRRVWLFI
                     IVNLIFSAFIAYVGYGLGTPQAFITPIDYFSKVIPALRNLTNYGFNSGTPYGLYGQWP
                     IFKNAVGVFCYYLFLVGFLWSGARWLLSKFKIVPFQNLQGAAVGAFLVTILIFDLPFL
                     ISINYIGRYFIPFIPFLAILSAFFVEDLIGMAKKYGINNFVSPILITILIAGVTYSAL
                     RLVSISLLFINDARIPATEYIASFRGYQKSIEYTLYPPYIEKRRFMRAHNYPIYFVEW
                     EGDTVPTGGRFEYNLGEQGLLERDTDYFVIDSFTYDRFYVDSICDTTPVECEFFLSLL
                     DNKVQTYQLLKSFTYELPTYLPDINLTAVNPKIYIYERVIE"
     gene            complement(2656..3399)
                     /locus_tag="PLT08_07180"
     CDS             complement(2656..3399)
                     /locus_tag="PLT08_07180"
                     /inference="COORDINATES: protein motif:HMM:NF019846.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HNE04283.1"
                     /translation="MGIERHKDLIDINQKLWHRKPILRTVYTDLYRIMQDYLTQVPGK
                     NLELGSGMGTIKEVMPDFLLTDLFHYPWLDLVENAYSLSFQNASISNLLMVDVFHHLR
                     YPGNALREFFRVLVPGGRLILMEPGLGLMGRVIYEWMHIEPVGKASNITWLAPEKWSP
                     NEMDYYAAQGNATWIFKKNLFSSHLSEWEIIETKNISALAYAASGGYSGPQLYPSRAY
                     PFIRSLERFLGLFPSIFTTRLLIVLEKRF"
     gene            complement(3402..>4033)
                     /locus_tag="PLT08_07185"
     CDS             complement(3402..>4033)
                     /locus_tag="PLT08_07185"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNE04284.1"
                     /translation="LLPYKEYKGVEKIYSVIFYYLPIFGRMYRKRVELCLNQCKGGES
                     ILEVGFGSGLTFINLSEKYTKIYGLDLTCDVKVVSKVFEPHKIQPDLQNGNVLSMPYE
                     DSKFDTVLLISILEHLKPIELEQAFKEIKRVLKPGGQVVYGVPVERPFMVFMFRLLGY
                     NIREHHFSTEIEVRETAEKILKKNHIHTMEASPVIFGSVYEIGHFTKVN"
BASE COUNT         1151 a          730 c          796 g         1356 t
ORIGIN      
        1 gggttgaatg ggtatccatt cctgtgctct gttattatca agagatacag acgtataaag
       61 aggttaatgt aatgctgctt attccaaaac cggtgccaga taattgcaca gactatattc
      121 caccaaaacc tgctgatcaa tagacttatg aaccttcgct atctttttct tcgactttta
      181 cgccatttta tgccagaagg gctagcacgc ttcttactta agcgacgctg gattattaag
      241 ccggggcttg aatccaccga cccatttgcc gcttccgcgc gctatgttaa tacactagct
      301 gcacagggag tttctattac tggaaaacga gtgctagtat ttggatacgg cggacgtttt
      361 gctgttgggg tagatctgct taagcgaggg gcagcgcatg tggttttgtg cgaccatttt
      421 gtgttgctag atacagaacg taaccgcgaa ttactgtcag cgtatgggca gtacctgaaa
      481 gtagaggggg atgaggtcca gccgcgaagt gaatttatca ccttattgca tggtgacatc
      541 cgtgaagaag ctatccaaaa acagatttca caggtggatg ttgtcttaag tacttcggtc
      601 tttgaacatt tagatgatgt gccggggatt acaaaagcgc ttgcaaaact gaccgcgtca
      661 caaggtatcc atttacattt tgtggacttg cgcgaccatt tcttcaagta tccattcgaa
      721 atgttaaaat actcggaaag cgtttggaaa cacttcttaa atcccaccag taatttgaat
      781 cgatatcgtt ggaatgatta taagcaagtc tttgaagaaa attttggaca ggtaagtttt
      841 atagtgattg aacggcttgt tgaagaattt agtaaagtga aatctaaaat tcgccctgaa
      901 tttagaactg gggatgagaa tatcgatgct gtgactctta ttcatgtaat agcaaaacag
      961 ccaaaatgat tggaattgaa gatgcataat aaattaaata cttttttcac gcggtatgaa
     1021 ttcttactac cgctttttgt tttcgctatt tttcttactg cttcattacc tggtaatagt
     1081 tggggtgccc ctgctttatg gaatcctgat gaattggtct ggcgcgtgga tatggcgctg
     1141 cacggtaata tgcgatttga tgtgaccgag ccggattata actatccctc tttacctaag
     1201 tatgtgatgt atgcaattgg ttatgtaacc tacgggttgg ggcgttctaa ttatgcattt
     1261 attgtggcag caagatcatt ttcggcattt ttaggtgctt tggctggggt cttgatctat
     1321 tatcttgccc gcctaattgg tgccaataaa agaactgcaa cattagctgg gctgttttat
     1381 gtaataagtg gtattgctgc tgaaaatggg cgttttgctc ataatgattt atatctacag
     1441 ttctttacta tcttaagcgt gttttttgtt attaagtatc agcatacaac aaaaaacaac
     1501 tggctttatc tttcattttt ttcggttggg cttgctgcgt catgtaagta tacgggcggt
     1561 agtttaatta tcctgccaat aattgttttt attgcggcaa attggtcaga tattcgtaga
     1621 gtgtggttat ttattatagt aaatttgatt tttagtgctt ttattgcata tgtgggctat
     1681 ggacttggta cgcctcaagc ttttattacc ccgattgatt acttttcaaa agttattcca
     1741 gcccttcgaa atttaactaa ctatggtttc aattcgggca caccatatgg tttgtatggt
     1801 caatggccca ttttcaagaa tgcagtagga gttttttgct attatttatt tttagtaggg
     1861 tttctttggt ctggtgcaag atggctatta tccaagttca agatagttcc atttcaaaat
     1921 ctacaaggag cggcagttgg tgcttttctt gtaacaatat tgattttcga tttgcctttt
     1981 ctgatctcca ttaattacat tgggcgttat ttcattccat ttattccttt tctagcaatt
     2041 ctgagcgcat tttttgttga agatttaatt ggtatggcta aaaaatacgg cattaataat
     2101 tttgtgtctc ccatcctcat aactatttta atagctggag ttacctattc tgcacttcga
     2161 ttagtaagca tttctttgtt gttcataaat gatgctcgta taccagctac ggaatatatt
     2221 gcatcttttc gtgggtatca gaaaagtata gaatatacac tgtaccctcc ctatattgaa
     2281 aaaagacgtt ttatgcgagc ccataattac cccatatatt ttgtggaatg ggaaggagat
     2341 acagtcccaa caggtggacg ttttgaatac aatcttggag agcagggtct tttggaacgt
     2401 gatacagatt attttgtaat agatagtttt acttatgatc gtttttatgt agactcaatt
     2461 tgcgatacta ctccagtaga gtgtgaattc tttttaagtt tacttgataa caaagtgcaa
     2521 acataccagt tgcttaaaag ctttacctat gaacttccaa cttacttacc tgatataaac
     2581 ttgacagcag ttaatcccaa aatttacata tatgaaagag taattgaata aagaaaagag
     2641 gttctgaagt atgcttcaga acctcttttc taaaacaatt aataaccgtg ttgtgaaaat
     2701 agaaggaaaa agccctaaga acctttcaag tgacctgatg aaagggtatg ctcttgaagg
     2761 ataaagttgt ggaccagaat atccgccaga agcagcatat gctagtgctg agatattctt
     2821 tgtttctatg atttcccatt cactaagatg tgatgaaaat aggttttttt taaatatcca
     2881 agtagcattg ccctgtgcag cataataatc catttcattt ggagaccatt tttctggggc
     2941 aagccaggtt atattgcttg cctttcctac tggttcaata tgcatccatt catatataac
     3001 tctccccatt agcccaagac cgggctccat tagtataaga cgaccacctg gaactaatac
     3061 tctgaagaac tctcttaaag cattcccagg atatcgcaga tgatgaaaaa catctaccat
     3121 gagtaagttt gatattgatg cattctgaaa agataatgaa taagcatttt ctactaaatc
     3181 taaccaagga tagtgaaaaa gatctgtaag taaaaaatct ggcataactt ctttaattgt
     3241 gcccatacca gaaccaagct ctaaattttt acctggaact tgggtaagat agtcttgcat
     3301 gatacggtaa agatctgtat atacggttct taatatgggc tttcgatgcc aaagcttctg
     3361 gtttatatcg ataagatctt tatggcgctc aatccccatg attaattaac cttggtaaag
     3421 tgcccaattt cataaacgct cccaaatatt acaggagatg cttccattgt gtggatatgg
     3481 tttttcttta ggattttttc tgctgtttca cgaacttcga tttcagtaga aaaatgatgt
     3541 tcgcgaatgt tataccctaa tagacgaaac ataaatacca taaaaggacg ttcaaccggc
     3601 actccataaa caacctgccc gcctggcttc aaaacgcgct taatctcttt aaatgcctgt
     3661 tcaagttcta taggttttaa gtgttcaaga atactgatga gtaaaactgt gtcaaatttg
     3721 ctgtcttcat atggcattga aaggacattg ccattctgta gatctggctg aattttatgt
     3781 ggctcaaaga ctttgctgac aactttgaca tcacaagtta ggtcaagccc ataaatcttt
     3841 gtgtattttt cactaagatt tatgaaagtt agtccgcttc cgaaacctac ttcaaggatg
     3901 ctttccccac ctttacattg attaaggcaa agttcaactc gtttacggta cattctccca
     3961 aaaattggta aatagtaaaa aatgactgag tagattttct ccactccctt gtattctttg
     4021 taaggtaata aac
//
LOCUS       DAOQJE010000222         4024 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_13648_length_4024_cov_5.350466, whole genome shotgun sequence.
ACCESSION   DAOQJE010000222 DAOQJE010000000
VERSION     DAOQJE010000222.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 4024)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4024)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4024
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13648_length_4024_cov_5.350466"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2058
                     /locus_tag="PLT08_07190"
     CDS             <1..2058
                     /locus_tag="PLT08_07190"
                     /inference="COORDINATES: protein motif:HMM:NF014763.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cache domain-containing protein"
                     /protein_id="HNE04285.1"
                     /translation="SIFIPTNVNLNNALAIKLNLLKHSELIFPPMLKGNPDIVAIYFG
                     GESKETIYYPNIDLANIVPADFDVTGRGWYLAAKPSNNPKGGVVWSAPYEDAALNGLV
                     ITTSAPVVDKIGRFQGVTAIDVKITRIIDLVANVKIGDTGYAFLIDDNNRLISLPETG
                     YNDFNITDENAKLSIIMDPAALSSVTPEFTDVMEKIKSQEQGIFSIVLNGAEKHITFH
                     NIPEVNYKLVYVIPSEELFSSRQLISEQISNETRSTINFSVLLIAIVFLVAIGTSFAI
                     NNSFTAPLQPLNEAASEIIKGNFDARVEVKSRDELETLATTLNTMTDTVKDLVASLEK
                     RVYERTIELQQANQEGEKRGKQYEAIAKVAQAISTQKNLQELLPQITNVISEQFGFYH
                     VGIFLNDALNKYAVLVAANSAGGKRMLKRGHQLKVGAQGIVGYATGMKRPRIALNVGD
                     DSVFFNNPDLPDTRSEVALPLMETGNILGALDVQSTEPNAFSEKDLEVLSVLAELVSI
                     AIENAKLYEKMDRSLAEAEAASRQFFRENWNRLAEEYRIAGYRYTAGGATSLSIAQED
                     VASSIPSDRRQVQVPIIMRGQEIGELSVVIPKDENIKADQMDLIKAVADRVAVIAENA
                     RLFDETTRRAERERLVTDISTKIRSTNDPQAMIETAINELREALKVSRIEIVPQKKNI
                     TDR"
     gene            2087..2872
                     /locus_tag="PLT08_07195"
     CDS             2087..2872
                     /locus_tag="PLT08_07195"
                     /inference="COORDINATES: protein motif:HMM:NF018290.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HNE04286.1"
                     /translation="MTKTIAVSNEKGGVAKTTTVLSLGAALAELGNRVLLIDLDAQAN
                     LTLALGLEPGETDLTSSNVMLDNEPILKVCRKTEIPDLDLIPSSSRIETSEQFLPMRT
                     NYTTILQRAIQSAPLPYDYIVLDCPPALGAITRNALAAANLLLIPTQAEYFSAYALRN
                     MMGLIRRTREMDNPNLSYRILVTMLDRRNRTHRNIHEQLRATFGSGVFNTVIELDTKL
                     RESPIAGQPITHYKSAARGSTQYRVLAQELMEYAKETENHQAS"
     gene            2838..>4024
                     /locus_tag="PLT08_07200"
     CDS             2838..>4024
                     /locus_tag="PLT08_07200"
                     /inference="COORDINATES: protein motif:HMM:NF024583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HNE04287.1"
                     /translation="MPKKPKITKRLDDLFKDVKPEENAKGRATKPKSQKEAPPPLPAD
                     PKPAQPAQAASTPVKTTRRQTGTLALPSKFIGRDTNAEAKSSYATTIQLGEEEWATLR
                     LVDNQSERSFSTDEQLLIRQVTDQLSLALENARLFQETQRRAQELEIVNKVVSAASGS
                     LDLQSTLQSVADNLVSLLDAQNVGIALINDEKTHLVLAAEAFSTLESSTGIGLLIPIE
                     GNLSTEEVLRTRKPLFIRDVDNNPLLVDSVREALQQRSTKNLLILPLNSEQEVIGTIG
                     VAFFDANRVIAQDELRILETIVAQIAAAVQKNRLFTQVEQSETNFRALFSAMEDVVLV
                     INREGRYMQIAPTNPSLLVRTPSDMLGKTFHELLPIDIADSFLSKVQEALVSDTKVQF
                     EYEL"
BASE COUNT         1172 a          983 c          865 g         1004 t
ORIGIN      
        1 tctatattta ttcctaccaa cgttaactta aacaatgcac tggcaatcaa attaaatctt
       61 ctaaagcatt cagaattgat tttcccgccc atgttgaagg gcaatcctga tattgttgct
      121 atctattttg gtggtgaaag taaagaaacc atctattatc ctaatattga cctggcaaat
      181 atcgtccctg ccgatttcga tgtgaccgga cgcggttggt atcttgccgc caagccaagt
      241 aacaacccca aaggcggagt tgtttggtct gccccatatg aagatgctgc actaaacgga
      301 ttggtcatta ccaccagtgc gcccgtggtc gataaaatcg gtagatttca aggtgttact
      361 gccattgatg taaaaatcac tcgtattatt gaccttgttg ccaatgtaaa aatcggtgac
      421 acagggtatg catttttaat tgatgataac aatcgcctca tttctctacc cgaaactggc
      481 tacaacgact tcaacatcac cgacgaaaat gccaaactca gcattattat ggaccccgct
      541 gctctatctt ctgtgactcc agaatttact gatgtcatgg aaaagatcaa atcgcaagag
      601 cagggcattt tctccattgt gctgaatgga gccgagaaac acatcacttt ccataatatc
      661 ccggaagtca attacaaact ggtttacgtc attccatctg aggaactgtt ctcaagtcgt
      721 caactcatct ccgaacagat atcaaatgaa actcgctcaa cgatcaattt tagtgttttg
      781 ttgattgcta ttgttttctt ggtcgctatt ggcacatcgt tcgccatcaa taatagcttt
      841 acagcgccgt tacagccact aaacgaagct gccagtgaga ttatcaaagg aaactttgat
      901 gcccgcgtag aagtaaaatc acgggatgag ttggaaaccc tagccacaac cctcaacacc
      961 atgaccgata ccgtaaagga cttggtcgct tctcttgaaa aaagagtgta tgagagaacc
     1021 atagaacttc agcaagccaa tcaagaaggt gaaaaacgcg gaaagcaata tgaagccatc
     1081 gccaaagttg cgcaggcgat cagtacccaa aagaacctgc aggaattatt gccgcaaatt
     1141 accaacgtga tcagcgagca atttggtttt taccatgtgg gcatcttctt gaatgatgca
     1201 cttaataaat atgccgtttt agtggcagcc aacagtgctg gcggaaaacg catgttgaaa
     1261 cgcgggcatc aattgaaagt tggcgcacaa ggcattgtag ggtacgccac agggatgaaa
     1321 agacctcgca ttgcccttaa cgtaggtgac gactcggtct tctttaacaa cccagatttg
     1381 cctgatactc gctctgaagt ggcattgccg cttatggaaa ctggcaatat cctcggcgcg
     1441 ctagatgtgc aaagcacaga gccgaacgcc ttctctgaaa aagatctcga agtgcttagt
     1501 gttctggcag aattggtaag tatcgccatt gaaaacgcaa aactctatga aaaaatggac
     1561 cgatcgcttg cagaagcaga agcggcttca cgccagttct tccgcgaaaa ttggaatcgc
     1621 cttgcagaag agtatcgtat tgcaggttat agatatactg caggtggagc cacatccctc
     1681 tctatcgctc aagaggacgt cgccagttcc ataccatctg accgaagaca ggttcaagtc
     1741 cccatcatca tgcgtggaca agaaataggc gagctttctg tggtcattcc aaaagatgag
     1801 aacatcaaag ctgaccaaat ggacctcatc aaagctgtag cagaccgtgt agccgtgatc
     1861 gcagaaaatg cccgcttgtt cgatgaaacc acacgtcgcg cagaacgcga acgcctggta
     1921 acagatattt caacaaagat ccgcagtacg aacgatccgc aagccatgat cgaaactgcc
     1981 atcaatgaat tacgtgaggc gctgaaggtc tcacgtattg agattgttcc gcaaaagaaa
     2041 aatattacag accgttagcg gtgttttagg aaatgaggaa tacaacatga cgaagaccat
     2101 agccgtatcg aatgaaaaag gaggagttgc caaaacaaca accgtactct cccttggtgc
     2161 agcattggca gaacttggca accgcgtcct tctcatcgat ttggatgcgc aagcaaacct
     2221 cactctggct cttggtttag agcccggaga gaccgatctc acctcaagca acgtcatgct
     2281 tgataacgaa cctattctca aggtctgtcg aaaaacagaa attccagacc tcgatcttat
     2341 cccgtccagt tcacgcattg agacctcaga acaatttttg ccaatgcgca caaattacac
     2401 caccattctt caacgtgcta ttcagtcggc accattgcct tatgattaca tcgtgctaga
     2461 ttgtccaccc gcattgggcg ccatcacacg caatgcgctg gctgctgcca atttattgct
     2521 tatccctacc caagcagaat atttctctgc ttatgctttg cgcaatatga tggggcttat
     2581 ccgccgcacg cgcgaaatgg ataaccccaa tctctcatat cgtatccttg ttaccatgct
     2641 agaccgacgt aaccgcacgc atcgcaacat ccatgaacaa ctccgtgcca catttggttc
     2701 gggggtcttc aacaccgtta tcgagttaga tactaaactt cgagaaagcc caatagctgg
     2761 acagcctatt acacattaca agtctgctgc ccgtggttct acccaatatc gtgtcctcgc
     2821 ccaggaatta atggaatatg ccaaagaaac cgaaaatcac caagcgtctt gatgatctct
     2881 tcaaggatgt aaaacctgaa gagaatgcca agggacgggc aaccaagccc aagtcacaga
     2941 aagaagcgcc accgcctctt cctgcggacc cgaagcctgc gcaacccgcc caagctgcat
     3001 ctacaccggt aaagacaacc cgtcgccaaa cgggtacgct tgctttgcct tcaaagttca
     3061 ttgggcgtga tacaaatgcc gaagcgaagt cttcgtatgc tacaacgatt caacttggag
     3121 aagaagaatg ggcaactctt cgccttgtag ataatcagtc tgaacgctca ttttcaactg
     3181 atgagcaatt gcttattcgg caagtgaccg accagttatc gctggcgtta gaaaatgcac
     3241 gtctgttcca agagacacag cgccgcgcac aagagcttga aatcgttaac aaggttgtgt
     3301 ctgcggcatc tggttcgctt gacctgcaaa gcaccttgca aagtgttgcc gataaccttg
     3361 taagcttgtt ggatgcacaa aatgttggta ttgcgctaat caacgatgaa aagacacatc
     3421 ttgtccttgc agcagaagca ttctcaaccc tagagagttc aactggaatt ggactgttaa
     3481 ttccaattga aggcaatctc agcacagaag aagttttacg cactcgtaaa ccattattta
     3541 tcagggatgt agataacaat ccgcttctag tggactctgt tcgagaagca cttcaacaac
     3601 ggagcaccaa aaatttactg atcttgccct tgaattctga gcaagaagtg ataggaacca
     3661 ttggcgttgc atttttcgat gccaaccgtg ttattgctca agatgaactt cgcattcttg
     3721 agaccattgt tgcccaaatc gctgcggctg tacaaaagaa caggttgttt actcaagtag
     3781 agcaatctga aacgaacttc cgcgcgctat tctcggcaat ggaagatgtg gtgttggtta
     3841 tcaaccgtga agggcgttat atgcaaattg cccccaccaa cccatctctc ttggttcgca
     3901 ccccttcaga catgctggga aagaccttcc atgagctttt gccgatagat attgccgact
     3961 catttctttc aaaggttcaa gaagctttag taagtgatac aaaagtacag tttgagtatg
     4021 aact
//
LOCUS       DAOQJE010000223         4012 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_13711_length_4012_cov_5.420268, whole genome shotgun sequence.
ACCESSION   DAOQJE010000223 DAOQJE010000000
VERSION     DAOQJE010000223.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 4012)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4012)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4012
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13711_length_4012_cov_5.420268"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            232..1119
                     /locus_tag="PLT08_07205"
     CDS             232..1119
                     /locus_tag="PLT08_07205"
                     /inference="COORDINATES: protein motif:HMM:NF019474.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HNE04288.1"
                     /translation="MLSWQSYIVRLYLNYSKATLDDNTPIEKLRQAVEDGARFSRFPK
                     SVQSQTVTIEHITAEWIIPSQADTRSVMLYLHGGGYALGSIKSHRATAGQIAEAGKIR
                     TLIIEYRLAPEYPFPAALEDSLIAYKWLRKNGYEKIIIVGDSAGAGLALSTVISLRDN
                     QISMPMQVICLSPWLDLEGTGESVITNSGRDPLFKADDLRLGKFYIGENNPHNPLISP
                     LYADFTRLSPILIHVGADEILLSDSTRLVQKAKDAGVDVQMKVWQGMWHIFPFFAPFV
                     PESSQAIIEIGDFIRHKID"
     gene            1382..1957
                     /locus_tag="PLT08_07210"
     CDS             1382..1957
                     /locus_tag="PLT08_07210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE04289.1"
                     /translation="MDKISFYFYIFSGAVFLLLLFAIITNDHKTKQWIFAASYSSVIL
                     VVLGFFISNLPSNELYTLATAIIAPQIIQLIANLRHKPIHKSSNPFKSKPFRWAAFLI
                     LIAISSIPFFTVIDNETNIAYFFRLSSGVFLFIFFILIYIQPDQADGIRTVYEHLEED
                     IPSLENQGWIIVDTTNKNVEATVRHILLQIK"
     gene            2203..2400
                     /locus_tag="PLT08_07215"
     CDS             2203..2400
                     /locus_tag="PLT08_07215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE04290.1"
                     /translation="MFLLMMSPILSIFVFSIAPFIGGYKGYKNAREDEDKIEQAMKGI
                     GIGVLIDIPFLCVILALFSTT"
     gene            2447..2890
                     /locus_tag="PLT08_07220"
     CDS             2447..2890
                     /locus_tag="PLT08_07220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE04291.1"
                     /translation="MADIQQLLKLLQSNKPDKRFEACEELRAKPSLPPEAIDAFYPLT
                     IDENQDVADAAQRAISTHAHQHPQSPLEDASLVPVRKTTVTDYAVGGFVVGTVVSGLL
                     IFLFFSTFGTCTEVCPFAMLVLSPILGVFIAIISGIVGARKTHKY"
     gene            3169..3663
                     /locus_tag="PLT08_07225"
     CDS             3169..3663
                     /locus_tag="PLT08_07225"
                     /inference="COORDINATES: protein motif:HMM:NF025160.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HXXEE domain-containing protein"
                     /protein_id="HNE04292.1"
                     /translation="MQSASLWLFFPIAITLHNLEEAIWLPAWTKYAKQFHKPMEANVF
                     YFAVIFVTILAYLSTFLAVAFPSSWLWKYIFHGFLGAMILNTIFPHLVSTIILRRYSP
                     GLVTGLFLLVPINSITLYQSVIFGHVKLSELMISILIVSVVLLSFLPLFFKIGEQLAK
                     QMKA"
BASE COUNT         1152 a          838 c          868 g         1154 t
ORIGIN      
        1 ctactgatac cgaagacaga agccgcctaa caaagcgtcc cgatagaacc gggatgatat
       61 gcagcaacct gaaaagcacc aggacaggct gtctggaaag tcaagagcaa aagcaccttt
      121 aaaaagaccg tctcaagcag gctggctgga cagattcgcc cggtccgtga gacagggcgg
      181 gtaacgtcaa gccgttgggc tgtttcttgc acaataaagg aggtcgagag tatgttaagt
      241 tggcaaagtt atatcgttag gttatatcta aattactcga aagccacact tgatgacaac
      301 actccaattg aaaaactccg tcaggcagtg gaggatggag cgcgtttcag ccgttttcca
      361 aaatcggtgc aatctcaaac tgtcactatt gagcatatta ccgctgagtg gattattcct
      421 tcgcaggcag acactcgttc agtgatgtta tatctacatg gtggcgggta tgctttaggg
      481 tctatcaagt ctcatcgagc aacggcaggg caaattgctg aagcaggaaa aatccgtact
      541 ttaataattg aatatcgcct agcgcctgag taccctttcc cagcagcact agaggattct
      601 ttgattgcct ataaatggct acgaaaaaat gggtatgaaa agattatcat tgttggcgat
      661 tcggcagggg ctggattggc actttctaca gttatttcct tacgggacaa tcagatatct
      721 atgcctatgc aggtaatctg tttatctccg tggcttgacc tagaaggaac tggcgaatct
      781 gtgataacaa attctggacg cgacccattg tttaaagcgg acgacctaag attagggaaa
      841 ttttacattg gagaaaataa tccgcacaat cctcttattt cacctctata tgcagatttc
      901 acaagacttt cgccaatcct gattcatgtg ggagcagatg aaattttact gagcgactcc
      961 actcggcttg ttcaaaaagc aaaagacgcg ggtgtagatg tgcagatgaa agtatggcaa
     1021 ggaatgtggc atattttccc atttttcgcg cccttcgtgc ctgaatcatc gcaggctata
     1081 attgaaattg gtgattttat tagacacaaa attgattaac ggcaacacag cccaacaaag
     1141 cgtcccgata gaactgggat gatatatgca cctgaccctc ggacgaatac cgaggacagg
     1201 cgctggggac tgtccggagg gcgtgcggca cgcttcgcgc aagcagtttt caacgccttg
     1261 tcttttttcc ctggactgtc acaggctggc tggacggctt cgcccggtcc gctagacggg
     1321 gcgggtctcc acagggcaag tcgtacgccg ttggcatgtt ctttccaaaa ggaggtaatg
     1381 aatggacaaa ataagctttt atttttacat tttttctgga gcagtatttc tattactgct
     1441 ttttgcaatc ataactaatg accataaaac gaaacaatgg atatttgcag cttcttattc
     1501 tagtgtaatt ttagttgttt taggtttttt tatttcgaac ttgccaagca atgagttgta
     1561 tactttagca acagcaatta ttgcgccgca aatcatccaa ttaattgcca atttacgcca
     1621 taagcccata cacaaaagca gtaatccatt taaaagcaaa ccatttagat gggcagcctt
     1681 cttaatattg attgcaatat caagcatccc attttttact gttatagata atgaaacgaa
     1741 tattgcatac tttttcagat tgtcaagcgg ggtattccta tttattttct ttatccttat
     1801 atatattcag ccagatcaag cagatgggat acgaacagtt tacgaacatt tagaggaaga
     1861 tattcccagc cttgaaaatc aaggctggat tatagtggac acaacaaaca aaaatgttga
     1921 agcaacagta agacatattc tcttacaaat taagtaaatt gagcgctact tcaaaactcg
     1981 gctggcggca tcgggtttcc caacaaagcg tcccgataga actggcagtt ttccctggac
     2041 tgtcgtccag gacaggctgg ctggacggct tcgcccggtc cacgagacag ggcgggtacg
     2101 cacagggcaa gccgttggga agttccttaa agagtttatt ccatatcttc atgcacttgt
     2161 agtttatact ctgaaccata tagcggctaa aggagaaaaa caatgtttct tctcatgatg
     2221 tctccaattc tatccatatt tgttttttca atagcaccat tcattggagg atacaaaggc
     2281 tataagaatg ctcgtgagga cgaagacaaa attgagcagg caatgaaagg tataggtata
     2341 ggagtattaa ttgacattcc ttttttatgt gttattttag ccctattttc tacaacctaa
     2401 atttcttgat cgcttaacgc aaggtcgatt aaattaagga tgatatatgg cagatattca
     2461 acaacttttg aaattgcttc aaagcaacaa acctgataaa cggttcgagg cttgtgaaga
     2521 attgagagca aagccatcac tcccgcctga agcgattgat gctttttatc ctctcacaat
     2581 agatgaaaat caagatgttg ccgatgcagc acagagagcg atatctactc atgctcacca
     2641 acatccacag tcaccccttg aagatgcttc cctagtccct gtccgaaaaa cgactgtcac
     2701 tgattatgcc gtaggtggtt ttgttgtcgg tactgtggtt tctggtttac ttattttctt
     2761 gtttttttca acctttggca catgcacaga agtgtgccca tttgctatgc tagtcttgtc
     2821 tccgattcta ggtgtattta ttgctatcat ttctggaata gtaggtgccc gaaagaccca
     2881 taaatattaa tataaggacg aatcaggctt taaggagtat ctcgtggttt tcatccagcg
     2941 cctaacaaag cgtcccgata gaactgggat gatatgcagc aaccctgaaa agcaccagga
     3001 caggctgtct tgaaagtcaa gagcaaaagc acctttaaag ggaaacccgg accgtctcag
     3061 gtcaggctgg ctgggcagct tcgcccggtc cgctagacag gacaggtccc cacagggcta
     3121 gccgcaaata cgtcaaatat caaagagtaa tttcatggag aaaaaataat gcagtctgcc
     3181 agtttatggt tatttttccc gattgctatt acccttcata atttagaaga agcaatttgg
     3241 ttgccagcat ggacaaaata cgcaaagcaa ttccataaac ctatggaagc aaatgtgttt
     3301 tattttgcgg tcattttcgt aacaattctt gcatatttat caacattttt agccgttgct
     3361 tttccgtcat cgtggctatg gaaatatatt tttcacggtt ttcttggagc gatgattttg
     3421 aatacaattt ttccgcacct tgtttcaaca attatcttgc gtagatattc ccctggttta
     3481 gttactggtt tatttctttt agttcccata aactcaatta ctctctatca gtcggttata
     3541 tttgggcatg tcaagttatc agaacttatg atttcaattc tcatagtttc tgttgttcta
     3601 ttaagttttc ttccgctttt tttcaaaatt ggcgaacaac ttgcaaagca aatgaaagca
     3661 tgaaaataaa attgtgatta aaatctgatt aggcgtattt ttagccaatg agtctccaag
     3721 tcaaaagccg cccaacaaag cgccccgata gaaccggcat ttttccctgg actgtcgtct
     3781 aggacaggct ggttggatga cttcgcccgg tccgtgagac agggcgggta cccacagggc
     3841 aagccgcaaa ccgttgggcg tttgagttca gtgcattctg cttagaaaaa aacttaagga
     3901 atcaaggaac aatgaatacc tatgtaatcc ttttacgcgg catcaatgtt ggcggcaaaa
     3961 atacagtacc aatggcgggt ttgagaaaat gtctggaaga acaaggattt tt
//
LOCUS       DAOQJE010000224         4000 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_13765_length_4000_cov_7.061850, whole genome shotgun sequence.
ACCESSION   DAOQJE010000224 DAOQJE010000000
VERSION     DAOQJE010000224.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 4000)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4000)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4000
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13765_length_4000_cov_7.061850"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..417
                     /locus_tag="PLT08_07230"
     CDS             <1..417
                     /locus_tag="PLT08_07230"
                     /inference="COORDINATES: protein motif:HMM:NF016115.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004061 - arylformamidase activity
                     [Evidence IEA];
                     GO_process: GO:0019441 - tryptophan catabolic process to
                     kynurenine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclase family protein"
                     /protein_id="HNE04293.1"
                     /translation="DLEGIVIRADYRKGLAVTVEAFKGKEIRGRAVLVHTGWAEHWAT
                     DAYFENHPFLTEDAAIYLRDCGVKLVGIDSHNIDDTRGNARPVHSVLLKNEIIVAEHL
                     CNLENVPDEGFTFTAAPPKVKGAGTFPVRAFVKIQG"
     gene            473..628
                     /locus_tag="PLT08_07235"
     CDS             473..628
                     /locus_tag="PLT08_07235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein"
                     /protein_id="HNE04294.1"
                     /translation="MLFSPKEKGQGLVEYALILVLVAIVVIAALMILGPIIGNVFSKL
                     NSSLASV"
     gene            829..984
                     /locus_tag="PLT08_07240"
     CDS             829..984
                     /locus_tag="PLT08_07240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein"
                     /protein_id="HNE04295.1"
                     /translation="MLFALEEKGQGLVEYALILVLVAIVVIATLLILGPIIGNVFSKL
                     NSSLVSI"
     assembly_gap    1329..1428
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(1434..2582)
                     /locus_tag="PLT08_07245"
     CDS             complement(1434..2582)
                     /locus_tag="PLT08_07245"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNE04296.1"
                     /translation="MKLRFQLAIFLFVRTVLNSIHRMVYPFLPIFARGLGIDLSTMSL
                     LMTGRSLLGATSPFLGPIADWRGRKFGMLSGLGIFILGIGLVALFPTFWALVIALGLA
                     LIGKYIFDPAMQAYFGDRIPYHQRGTVLAVTEVSWSMAFIVGVPIAGFLIDRYGWAAP
                     FPLFSILGLIAFFILWRIIPNEDSHHEPVNNTREGYAAIIKSMPALAGVSIALWASAA
                     NELVNVIFGVWLEDSFGLKIAALAGASAVIGISELSGEGLVALTTDKLGKPRALAIGL
                     VANSLAAGLLPFIGRTQVGALIGLFLFYITFEYVMVSHIPMVTELVPAARATLLSLNV
                     TGHALGRALGAFLAAIIYQQFGFIFVALSAVVFNIIGIFALRKMQQAE"
     gene            complement(2591..3199)
                     /locus_tag="PLT08_07250"
     CDS             complement(2591..3199)
                     /locus_tag="PLT08_07250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE04297.1"
                     /translation="MKNFKSPLTYFFLTVGLIALLAFFGPEEKSLGSNVRLVYIHGAW
                     VLTAEIAFIFAALAGLLGLLLRRDLFHTWSAALGRTGIIFWVTYLPLSLLAMQTNWNG
                     LFLAEPRFRLAMIFAVTGILLQLGLWMFSIPWLTSLANIFYIIVLRVIFAQAQYIMHP
                     PPSPIFNSGNYVIIGFFVSLNLLAWLAAYFLTRFLLNLKPQN"
     gene            3321..>4000
                     /locus_tag="PLT08_07255"
     CDS             3321..>4000
                     /locus_tag="PLT08_07255"
                     /inference="COORDINATES: protein motif:HMM:TIGR03716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF475 domain-containing protein"
                     /protein_id="HNE04298.1"
                     /translation="MEYWPVVVIILQLIFLEGILSIDNAAVIGALVSPLPNDEEVPWP
                     GKLTWLGKALHPILGFQRMAALRVGLLGAYVGRGAMLFFTSFLIHNSWIKLVGAAYLI
                     HLAFDNLDDMAMGGDENGEVSPIEARSFWATVLTVEIMDLVFSIDNVVAAVSLSDKLW
                     VVMLGVGIGILTMRYAAGIFSYAVEKEPILKQAAYILVLNIGVELILSQVWKIEIPDL
                     LRFGISVAT"
BASE COUNT         1136 a          775 c          947 g         1042 t
ORIGIN      
        1 gaccttgaag gcatcgtcat ccgcgcggac tatcgcaagg gattggctgt caccgttgaa
       61 gccttcaaag gcaaagagat tcgcggacgc gctgttttgg tccacaccgg ttgggcggaa
      121 cattgggcaa ccgatgcgta ttttgaaaac catccctttt tgaccgaaga tgcagcaatt
      181 tacttgcgtg attgcggcgt gaagcttgtt ggtattgact cgcataacat cgacgatacc
      241 cgcggcaatg caagaccggt ccattcggtg ctgcttaaga acgaaatcat cgttgccgag
      301 catttatgca atttggaaaa tgtccctgac gagggattca ccttcacagc tgccccgcct
      361 aaggtcaaag gcgccgggac ttttcccgtc cgcgcatttg tgaaaattca aggttaagaa
      421 gtggggtaaa atcaagtttg taattatctt cagaggaagg aggtaaattg ccatgttatt
      481 ctcgcctaaa gagaaaggac aaggtttggt tgagtatgct ttgattcttg ttttggttgc
      541 catcgttgtt attgccgcac tcatgatcct tggtccaatc attggtaatg tgtttagtaa
      601 gctaaactct agtctcgcca gtgtctaaga gctactacag ttacatataa atccgtgttc
      661 aaagactgag cgcggattta ttttttcaat tcagacaaat tttataacct ttcaaatctc
      721 ttaagaaaaa ccattaaaaa gagattgaaa cccttactgt tttcctgcaa aatgatttca
      781 actctcgcat aaaagtgaga aattcaaaaa gaaaggaggt ggattattat gttatttgct
      841 ctcgaagaga aagggcaagg tttggttgag tatgcattaa ttcttgtttt agttgctatt
      901 gttgttattg cgactctttt gatccttggt ccgatcattg gtaatgtttt tagtaaactc
      961 aactccagcc ttgtaagtat ttaaagttcc acaataagtc attattatcg gggtaataat
     1021 gacttattgc actctactag tcaacctcat catccttgta aggttaacat aattgttaag
     1081 gaataacata cagggtctat tgcaaaaaaa acaagtccat gtaaaatact agtatctcag
     1141 gatgaccttc tgggaaatac tgaagaaagg agaaaattcc catgttattt tcacccaagg
     1201 aaaaaggtca gggtttggtc gaatatgcgt tgattcttgt tttggtagct atcgttgtta
     1261 ttgcggctct tatgatcctc ggcccgatca tcggtaacgt cttcagcaag attaactcca
     1321 gcctcggcnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1381 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnngg catttactct
     1441 gcttgttgca tttttctcag tgcaaagatt cctataatat taaacacaac agcactcaat
     1501 gcaacaaata tgaatccaaa ctgttggtag attattgcgg ctaaaaatgc gccgagtgcg
     1561 cgtcctaagg catgaccagt tacattgagc gatagcagag ttgcacgtgc agcagggaca
     1621 agttctgtca ccatggggat atggctaacc atgacatact cgaacgtaat gtagaagaga
     1681 aacagaccaa tgagtgctcc aacttgagta cgtccaataa acgggagtaa gccagctgct
     1741 aatgaattag caaccaagcc gatagcaaga gcacgcggtt ttcctagctt atcggtagta
     1801 agagccacaa gaccctcacc gctcaattct gaaatgccaa taactgccga agcaccagct
     1861 aaagccgcga ttttcaaccc aaatgaatct tccaaccaga caccaaaaat cacattgacc
     1921 aattcatttg ctgcactagc ccataacgca atagaaacac ccgctagtgc aggcattgac
     1981 ttaataatag ctgcataacc ttcacgggta ttattgactg gctcatgatg cgaatcttca
     2041 ttggggatga tacgccagag aataaaaaag gcgatcaacc ctaaaatgct gaataagggg
     2101 aatggcgcgg cccatccata acggtcaatt agaaaccctg caatgggcac acctacaatg
     2161 aatgccatag accaggaaac ttctgtaact gcaaggacgg ttccgcgttg gtgataagga
     2221 atgcgatcgc caaaataggc ttgcatggcg gggtcaaaaa tatatttgcc gatgagtgcc
     2281 agccctaatg cgatgaccaa tgcccaaaag gtagggaaga gcgccacaag acctatgcct
     2341 agaataaaaa tccccaagcc tgataacatg ccaaattttc gcccacgcca atcggcaatg
     2401 ggacctagaa atgggctggt agcaccaaga agggaacgcc cggtcatgag cagagacatg
     2461 gtggagaggt ctatgccaag cccacgagca aagataggca agaacggata gaccattctg
     2521 tgaattgaat ttaacacagt gcggacgaat aaaaagatgg caagttgaaa gcgtagcttc
     2581 acaagaatgc tcagttctgc ggtttgaggt taagcaagaa gcgggtgagg aaataagctg
     2641 caagccaagc aagcagattc aaagatacga aaaagccgat gatgacgtaa tttccagagt
     2701 taaagattgg ggatggaggc gggtgcatga tgtattgggc ttgcgcaaag ataacccgca
     2761 gaacaataat ataaaaaata ttcgccagcg atgtgagcca cggaatactg aacatccaca
     2821 gacccaattg gagtaggata cctgtaacag caaagatcat ggcaagacga aaccgcggct
     2881 cagcaagaaa gagaccgttc cagttggtct gcatggctag caaggacaga ggcagatatg
     2941 tgacccaaaa aataatgcct gtgcgaccaa gagctgcgct ccaggtgtgg aaaaggtcgc
     3001 gtcgcaggag caagcctaaa agtccggcaa gagccgcgaa aataaaggcg atttcagcgg
     3061 tgagtaccca agctccatgt atgtaaacga ggcgaacgtt tgatcctaat gatttttctt
     3121 cgggaccaaa gaaggctaga agggcgatca agcctacggt taaaaagaaa taggtgagag
     3181 gggatttgaa gtttttcatg gttggatttt aacattgcta tttatttgtt acaaaaattt
     3241 tcaaaagaaa gaggaaaatc tttgcggttt gggatagaat ccatacttgc gagtttatgg
     3301 agtgaaaggc tccttgtgat atggaatatt ggcccgttgt tgtaattatt ttgcagttaa
     3361 tttttttaga ggggattctt tcaattgata atgcggctgt gatcggtgcg ttggtgtcgc
     3421 cgttaccgaa tgatgaagaa gttccttggc ccggtaagtt gacatggctt gggaaggcat
     3481 tgcatcccat cctcggtttt cagcgtatgg cagccttgcg tgtgggctta ttaggtgctt
     3541 atgtaggacg tggagctatg cttttcttca ctagctttct catccacaat tcgtggatta
     3601 agctggtggg cgctgcttat ttgatccatt tggcatttga taatcttgac gacatggcaa
     3661 tgggtggaga tgaaaatggg gaggtttctc ctattgaagc gcgttcattc tgggctacag
     3721 ttttaaccgt tgaaataatg gacttggtct ttagcattga taatgttgta gcggcggttt
     3781 cgctttctga taaattatgg gtggtaatgt tgggtgtagg cattggcatt ttgaccatgc
     3841 ggtatgccgc tggtattttt agttatgccg tagagaaaga gcccattctg aagcaggctg
     3901 cgtatatcct tgttttgaat attggtgttg aactcatcct ttcgcaggtg tggaaaattg
     3961 aaatccctga tttgctgcgg tttgggattt cagttgcgac
//
LOCUS       DAOQJE010000225         3996 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_13783_length_3996_cov_5.938594, whole genome shotgun sequence.
ACCESSION   DAOQJE010000225 DAOQJE010000000
VERSION     DAOQJE010000225.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3996)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3996)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3996
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13783_length_3996_cov_5.938594"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1157)
                     /locus_tag="PLT08_07260"
     CDS             complement(<1..1157)
                     /locus_tag="PLT08_07260"
                     /inference="COORDINATES: protein motif:HMM:NF013109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015116 - sulfate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SulP family inorganic anion transporter"
                     /protein_id="HNE04299.1"
                     /translation="MPRRSLARLYRDEFAGYSLATFRNDLVAGLNVALVALPLALAFG
                     AASGATAAAGLVTSIVGGIIIGLLAGAPYQMTGAKAVISVVLIVLVQQYGLEGMWVAL
                     FMAGLLTLGIGLFRLGRFIAFIPSPVIAGFTAGISAIIIIRQFDPLLNIHTPTTITAA
                     QKLIGYFTTPNLSPDLHSLLMGLFVMGIMILYPTSWNKRIPGSLVALVIATASNSALG
                     WSIPTIQNIPRTLFLQPNFSIAEIPWQNISDFIAPALTLTALGAVETLLTGSIGAELT
                     GKRLQVNQHLIAQGIGNMILPFFGAIPTTSGIIRTKVAIQSGGQTRLTGIIHSLILLI
                     VMFAFADYLAMIPLSALASVLMVTAIRSVDLSSVKFTFGNFFKTDMIAFTVA"
     gene            complement(1318..2103)
                     /locus_tag="PLT08_07265"
     CDS             complement(1318..2103)
                     /locus_tag="PLT08_07265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE04300.1"
                     /translation="MMRTKAIYPILVLLCFSFACQALFPATPPPRDGVVIDNCSEFVK
                     SIRSMQTNDTPQELIETGIKQGGEFDVNEYFNVTPNLSMQEGYALDYVIIPDSLGSFP
                     LLAARPVDQPPYKSSSEVSVDSELTNYWKYMDVNDNEQGYFEYASFLLLANQFYLVWH
                     ANYNDLGIVCDRATVDNIVANVNDGGFGVEFDREQMKQIKALQNIEPLVKLTDTTATV
                     ELVVFSNWGGFYRMTYTISRSSPHEIMDRQDENIIPYDCGIMF"
     gene            complement(2165..2527)
                     /locus_tag="PLT08_07270"
     CDS             complement(2165..2527)
                     /locus_tag="PLT08_07270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE04301.1"
                     /translation="MSHTCEAIVVHCMDYRLQSYINQWLETNLGKGSYDRAVMAGGVY
                     DVYDVIRQVDISARLHGITKVVLINHEDCGMYGPGGTEERHEEDLDKAEDKIRRLFPH
                     LEVDSYYLNLDGTFERKS"
     gene            complement(2544..3581)
                     /locus_tag="PLT08_07275"
     CDS             complement(2544..3581)
                     /locus_tag="PLT08_07275"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HNE04302.1"
                     /translation="MTQFLTLSPHSPSPELIRQAADVILRRGLVAFPTETVYGLGANA
                     LDEEAVAKIFHAKGRPSNDPLIVHVASLDDVKLVAAEIPPIVEKLAAAFWPGPLTLVL
                     PKQAHVPASVTAGLDSVAVRVPSHPVALALIRASGVPIAAPSANRFGHTSPTTAQHVM
                     DDLNGRIDMILDGGATSVGVESTVLDVTCDPAMILRPGGVSREAIEKVIGVVDLRNNR
                     PNSDDEAQVSPGLLGKHYTPNAELILCKGERDSALEKLLGLAKQAQAEGRSVGLLLAN
                     EDMASFESACIPYPLGASNDLEALARNLYAGMRALDDQGVKLILTRDFGKQGLGLAIH
                     DRLLRAASQII"
     gene            complement(3581..3958)
                     /locus_tag="PLT08_07280"
     CDS             complement(3581..3958)
                     /locus_tag="PLT08_07280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE04303.1"
                     /translation="MPTKKPIIHCDALVVHCMDYRLQKFIQPWITVRFGYDNFDIISL
                     AGSVHDYEMVLKYVQLAVQIHTIETVCLINHEDCRAYGRDGTYKRHKHDLTDAQKKIK
                     ALFPHLKVETFYLHLDGTFEPIS"
BASE COUNT         1073 a          935 c          987 g         1001 t
ORIGIN      
        1 gcaactgtga aagcaatcat gtcagttttg aagaaattgc caaacgtaaa tttgacggag
       61 cttaagtcta ctgagcggat ggcagtgacc atcaagacac tagccagcgc agagagcggg
      121 atcatggcaa gataatcggc aaaagcgaac atgacaataa gcagtatcaa agagtgaata
      181 atgcctgtca ggcgcgtctg tccgccagat tgaatggcaa ctttggtgcg aatgattcca
      241 gatgtggtgg gaatggcacc aaagaatggc aggatcatat tcccaatacc ttgggcaatc
      301 agatgttgat tgacttgcag tcgtttgcct gtcagttcgg cgccaatgga tccggtaagg
      361 agggtttcaa cagcaccaag ggcagtgaga gttaaagccg gcgctatgaa atctgaaatg
      421 ttttgccatg gaatttctgc gatgctgaaa tttggttgca ggaataaggt tcgggggatg
      481 ttttggatgg tggggatgga ccagcccaga gcggagttgg aggcggtggc aattactaat
      541 gcgaccagcg aaccgggaat ccgtttgttc cagctggtag ggtagaggat catgattccc
      601 atcacaaata aacccatcag aaggctatga aggtctgggg agagattggg agtggtgaaa
      661 tatccgatca gtttttgtgc ggctgtgatt gtggtagggg tatggatgtt gagcagcggg
      721 tcgaattgac ggataataat gatggcggaa atccctgcag taaaaccagc aataacaggg
      781 gatgggataa aagcgatgaa acgcccgagc ctaaatagac cgatgcctag tgtgagcaaa
      841 cctgccatga agagcgctac ccacattcct tcaagaccat attgttgaac gagtacgatc
      901 aacaccacac tgataacggc ttttgcgccg gtcatttgat agggcgcacc tgccagaagt
      961 ccaatgatga tgccgccaac tatggaagtg actaatccgg cggcggcggt tgcccccgag
     1021 gcagcaccaa aggcaagcgc aagcggcaaa gcaaccagcg caacatttag acctgcaacg
     1081 aggtcattgc gaaatgtggc gagcgaatag cccgcaaact cgtccctgta aagacgggca
     1141 agggaacgtc ttggcatatt taattctccg taaatagtag tacaccaaag gctcggtgct
     1201 cccccaaggc gtcctttggt ttatgtcggg cggtatttta tcactgctga ttttattttg
     1261 tatacggatt taatagacta cacggtattc gtaatgaatc cgtgtagtct ttttattcta
     1321 gaacataatc ccacaatcat acggaataat attctcatct tgtctatcca taatctcatg
     1381 cggagacgat ctgctaatcg tataggtcat gcgataaaag ccaccccaat tgctaaacac
     1441 aaccaattct acggtggcag tcgtgtccgt cagcttgacg agtggttcga tattttgcag
     1501 agccttgatc tgtttcattt gttctctatc gaactccacg ccgaaccctc cgtcgttgac
     1561 gtttgcaaca atattatcaa cggttgcacg atcgcagact atacccaagt cgttgtaatt
     1621 ggcatgccaa actaggtaga attgattggc aagaagcagg aaggaagcgt attcaaaata
     1681 tccctgctcg ttatcgttga cgtccatata cttccaataa tttgtgagtt cagaatccac
     1741 ggagacttcg ctcgatgatt tatatggtgg ttgatctacc ggacgagcag ccaaaagcgg
     1801 aaaggaacct aatgaatcgg ggataatgac ataatccagc gcatatccct cttgcataga
     1861 aaggtttggt gtgacgttga agtattcatt cacatcaaat tcaccccctt gtttgatgcc
     1921 cgtttcgatc aactcttgag gcgtatcgtt tgtctgcata ctgcgaatcg acttaacgaa
     1981 ctctgaacag ttgtcgatca caaccccatc acggggtggc ggtgtagcag gaaagagcgc
     2041 ctgacacgca aaagaaaagc acaacaaaac aagaattgga taaattgcct ttgttctcat
     2101 catatgctcc aaataaaagg gttgagaaaa agacgaaagc cgttctgaaa atgttcctta
     2161 ttggctaaga cttacgctcg aaggttccat ctaagtttag gtaatatgaa tctacttcaa
     2221 ggtgtgggaa taacctgcga attttatctt cggctttatc aaggtcttct tcatggcgtt
     2281 cttccgtccc accaggacca tacataccgc aatcttcatg attgatcagc accacctttg
     2341 taataccatg caaccttgct gaaatatcca cctgacgaat cacatcataa acatcgtaca
     2401 caccacctgc catcacggcg cggtcgtaac tccctttgcc taaattcgtt tccaaccatt
     2461 ggttgatata agactgtaaa cgataatcca tgcaatgaac cacaattgct tcacacgtat
     2521 gagacatttc ttccctccga aaattaaatg atctgactcg ctgctcttaa taagcggtca
     2581 tgaatagcca gccccaaacc ttgttttcca aaatcacgtg tcagaatcaa cttcacacct
     2641 tgatcgtcca acgctcgcat gcccgcataa agattccgcg caagcgcttc gaggtcattg
     2701 gatgcaccaa gcggatatgg aatgcaagcc gactcgaaag acgccatgtc ttcatttgca
     2761 agaagaagtc ccacgcttct tccttccgct tgtgcctgtt tcgccaatcc cagcagtttt
     2821 tccaacgctg aatccctttc gcctttgcat aggatcaatt ctgcatttgg cgtgtaatgt
     2881 ttccccaata accccggcga gacttgtgcc tcgtcatccg agttggggcg gttattgcgt
     2941 aaatccacca caccgatcac tttttcaatt gcctcacgcg aaaccccgcc cggacggagg
     3001 atcatcgccg ggtcacaagt cacatccagc accgtagatt caactcccac tgaagttgca
     3061 ccgccatcga ggatcatgtc aatgcgaccg ttgagatcat ccatcacatg ttgagccgtg
     3121 gtcgggctgg tatgcccgaa gcgatttgca gatggcgcag caataggcac accactcgca
     3181 cggatgagcg ccaacgccac agggtgtgac gggacacgta ctgcaactga atctaaccct
     3241 gctgtgacag atgcggggac atgcgcttgc tttggcagca ccaacgtcag cggaccgggc
     3301 cagaaagcgg cggcgagctt ttcaacgatg ggggggatct cagcggcaac gagttttaca
     3361 tcatcaagcg aagccacatg cacaatcagc gggtcattgg aagggcgccc tttggcatgg
     3421 aaaatttttg ccacagcttc ctcatcgaga gcgttggctc caagtccgta tacggtttcg
     3481 gtggggaagg caaccaagcc gcggcgtagg atcacgtctg cggcttggcg aatcagttca
     3541 gggcttgggg aatgaggcga aagggtcagg aattgggtca tcaagagatt ggttcgaagg
     3601 ttccatccaa gtgcagatag aacgtctcaa ctttcaagtg tggaaataaa gctttgattt
     3661 ttttctgagc atcggtcagg tcatgtttgt ggcgtttata tgtgccgtct cgtccatagg
     3721 cgcggcaatc ttcgtggttg atcagacaga cggtttcaat ggtatggatt tgcacagcaa
     3781 gttggacata cttcaatacc atttcatagt catgtacact acctgccagt gagatgatgt
     3841 cgaagttgtc gtatccaaag cggacggtga tccacggttg gatgaatttc tgcaggcggt
     3901 agtccatgca gtgaacaacg agtgcgtcgc agtgaatgat cggttttttg gttggcataa
     3961 tgtttttacc tctaatatgt aatgttgctc aattca
//
LOCUS       DAOQJE010000226         3993 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_13796_length_3993_cov_6.341290, whole genome shotgun sequence.
ACCESSION   DAOQJE010000226 DAOQJE010000000
VERSION     DAOQJE010000226.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3993)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3993)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3993
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13796_length_3993_cov_6.341290"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1730
                     /locus_tag="PLT08_07285"
     CDS             <1..1730
                     /locus_tag="PLT08_07285"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015662 - P-type ion transporter activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0043169 - cation binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HNE04304.1"
                     /translation="KVVDAKQYPAILSTLWLGVLNNDAEIEITGETDSQKTYRIVGDP
                     TEGSLLVAAAKAGAIHVEIDEAYPRENEVPFDSERKRMITIHDVSSPNPNDPSPFTNE
                     KHKDWDVIAVKGAPDIVLTLCSKYQGMDDQPRELTDDMRSKILGANDAMTKDALRVLG
                     FAYRLEKNVPDNLENIKTEDLEKDLVFVGLMGMIDPPRTEVKPALEKARHAGIRTVMI
                     TGDFPNTAKAIAESIGLLRPGKGVMTGAELDKIDDTQLKNIIEDTDVFARVSPEHKMR
                     IVDALQANDEVVAMTGDGVNDAPAIKRADIGVAMGITGTDVAKGTADMVLTDDNYASI
                     VSAVEQGRIIYSNIRKFVFFLLSSNVAEIMIIFLATLAGLPAPLTAIQLLWLNLITDG
                     APALALAMEKGDPDIMDQKPRAKSEPIVNRSMGIGIVVQTIMQTSAVLIAFVMGLAWH
                     LESGAIIPSGANFLSHVLAHDWRGVDVQTAETMAFATLSMAELFRAYTVRSERASVFK
                     IGIFSNKYMQYAVGISMVLLLLVCAVPFLQPIFNTHFLSLREWGLVVGLSLMPATAEE
                     VTKFFLRKG"
     gene            1825..3369
                     /locus_tag="PLT08_07290"
     CDS             1825..3369
                     /locus_tag="PLT08_07290"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HNE04305.1"
                     /translation="MKKEKRTLTLNEWIWLILGSAVILGSIVRLLPGVLAGFPLNDGG
                     MFLSMVKDLQTSHYVLPKFTTYNYLNIPFAYPPFGFYFARAVSDILSIPELALFRWVP
                     VLVNSFSIFVFYLLASEIAKSKLAGAISSVLYALTPGAFGWFIMGGGLTRSFGSLFLL
                     LTVYSTLKLFREDEKKYIGLSILFGGLTVTSHPEAGIHAAIGCVLVWVFFGRTNKSLL
                     QAIAIGLGVFLFISPWLASVLHYHSVTPILSALHTGSSGNNFIGSIYDLLFDEGIIPI
                     LFLFRITGLIWGVSKKHYFLLVWSFLPYLVEPRSAPSVSFYPMTILTALAFIEALPWL
                     LGRIKKQEIQNLHQNVIFNIFLLTVISLLFIDSCLYGFRLVGNSLKPNDLETMYWVKE
                     NLPENSVFMVLTGIPSPEIDPYVEWFPALAERQNISTLQGYEWLLADKFYNFYTELSK
                     LQQCRSVDCIEKWNTTNSQRYDYVVIFRTEAVEIDELLLKNSSYEKVYSNSNALIVKY
                     DESSTK"
     gene            complement(3382..3801)
                     /locus_tag="PLT08_07295"
     CDS             complement(3382..3801)
                     /locus_tag="PLT08_07295"
                     /inference="COORDINATES: protein motif:HMM:NF024845.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-TFIIB domain-containing protein"
                     /protein_id="HNE04306.1"
                     /translation="MNCPKCSSELTKKYYKGMFEVDSCPNCRGMWLDFNELDKLEDVI
                     FDEDEHKGSLVHFQTRTEFPCPHCGMLLDEFKYRLYDLKLETCAENNHGFWLDAGEDE
                     RVMEIMQKRAGDMQRKINAETEWKQILKGMHEFLGRK"
     gene            complement(3782..>3993)
                     /locus_tag="PLT08_07300"
     CDS             complement(3782..>3993)
                     /locus_tag="PLT08_07300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE04307.1"
                     /translation="QVLLDVLHYAKAEGFGLLGLVKSSLLGPKGNAEFLVWLNMKPND
                     VLAEDLVNSIFPASEMDKNDELSKV"
BASE COUNT         1057 a          899 c          845 g         1192 t
ORIGIN      
        1 gcaaagtggt ggatgcaaag caatatcccg ccattctttc cacactgtgg cttggggtct
       61 tgaacaatga tgctgaaatt gagatcacag gcgagaccga ctcacagaag acttaccgta
      121 ttgtaggcga ccccaccgaa ggctctttgc tggttgcggc ggcaaaagct ggtgctattc
      181 acgttgaaat tgacgaagcc tatccgcgtg aaaatgaagt cccatttgac tcagaacgca
      241 aacgcatgat caccattcat gatgtgagca gccccaatcc caatgatcca tctccgttca
      301 ctaatgaaaa gcataaagat tgggatgtca ttgccgtaaa aggtgctcct gatatcgtgt
      361 tgacactctg ctcaaaatat caaggcatgg atgatcagcc acgtgaatta acggatgaca
      421 tgcgtagcaa aattcttggc gcgaatgatg ccatgaccaa agacgctctg cgtgtgcttg
      481 gctttgccta tcgtttggaa aagaatgtac ccgataacct tgaaaatatt aagaccgaag
      541 accttgaaaa agatttggtc tttgttggtt tgatgggcat gatcgaccca ccgcgcaccg
      601 aagtaaaacc tgcactcgaa aaggcacgcc atgcgggcat ccgcacagtg atgatcacag
      661 gcgacttccc aaacactgcc aaggcgatcg cagagtcgat tgggttgttg cgacccggca
      721 aaggcgtcat gaccggagcc gagttggaca aaatcgacga tactcagctc aagaacatca
      781 tcgaagatac agacgtcttc gctcgcgtct ctcctgaaca caaaatgcgt attgtggatg
      841 cgttacaggc gaatgatgaa gttgttgcca tgactggcga tggcgtgaac gacgcgccag
      901 ctatcaaacg cgcagacatc ggcgtcgcaa tgggtattac tggcaccgac gtcgccaaag
      961 gcactgctga tatggttctc acagacgata actatgccag catcgtctct gctgtagaac
     1021 agggacgcat catctactcg aacattcgga agttcgtgtt cttcctgctt tcgtccaatg
     1081 tggctgagat catgatcatc ttccttgcga ctctcgcggg tctacctgcg cctttgactg
     1141 ctattcaact cctgtggctt aaccttatta cagatggtgc tcccgcattg gcgcttgcca
     1201 tggaaaaagg cgaccccgat attatggatc aaaagccgcg tgctaaatct gagcccatcg
     1261 tcaatcgttc catgggtatt ggtatcgttg tccaaaccat catgcaaaca tctgctgtat
     1321 tgatcgcctt tgttatgggg ttggcgtggc accttgaatc aggagcgata atcccatctg
     1381 gagcaaattt cctgtctcat gtccttgccc atgactggcg cggcgtggat gtgcaaacag
     1441 cagaaacaat ggcgtttgcg accctttcaa tggcagaatt gttccgtgcg tacacagtcc
     1501 gttcagagcg tgcttctgtt ttcaaaatcg gcatcttctc aaataaatat atgcaatatg
     1561 ctgtaggcat ctccatggtg ttgttactct tggtttgtgc cgtaccgttt ttgcaaccta
     1621 ttttcaatac acacttcctg agcctgcgtg aatggggact ggtggttgga ttgtcactta
     1681 tgccagctac cgcagaagaa gttacaaaat ttttcttaag aaaaggctag cactaaaaga
     1741 gccgcccttc gttttacgaa aggcggctct tttttatgac atagtgtatt attcattaca
     1801 ttaataacaa tctacttaaa cattatgaaa aaagaaaaac gcaccctaac cctcaatgaa
     1861 tggatctggt taattcttgg ttcagcagtg attcttggaa gcattgttcg tcttttgcct
     1921 ggcgtcttag caggttttcc acttaatgat ggaggcatgt ttctaagcat ggttaaagat
     1981 ctacagacca gtcactatgt tcttccaaaa ttcactacat acaattatct aaacatacct
     2041 tttgcatacc caccttttgg gttttacttt gcgagggcag tttcagatat cctttccatt
     2101 ccagaattag ccttatttcg ttgggtgcca gtactggtta attctttttc gatttttgtt
     2161 ttttacttat tggcatccga gatagccaaa tcaaaactag caggggcaat ctcgtctgtg
     2221 ctctatgcat tgacgcccgg tgcatttggt tggtttatta tgggcggagg gctaacacgc
     2281 agttttggct ctttattttt gcttttaact gtctactcca cactaaagtt atttcgagag
     2341 gatgagaaaa aatatatcgg tttatcgata ttatttggcg gtttaaccgt tacgagtcac
     2401 ccagaagcgg ggatccatgc cgctataggc tgtgttcttg tgtgggtttt ctttggtcga
     2461 acaaacaaat cccttttgca agctattgca attgggctag gcgtttttct tttcatatct
     2521 ccatggctag caagtgtatt gcactatcat agtgttactc ctatcctttc ggctctacat
     2581 acgggatcca gtgggaataa ttttatcggc tcaatctatg acctattatt tgacgaaggt
     2641 attattccga tcttgtttct ttttcgtatt acaggcttaa tatggggagt ttcaaaaaaa
     2701 cattacttcc ttttggtatg gtctttttta ccttatcttg ttgagccacg cagcgcgcca
     2761 agcgtatcgt tctatccaat gacaatacta acagcgctgg cgtttattga agctttgccg
     2821 tggctacttg gacgaataaa aaaacaagag attcaaaatt tacatcaaaa tgtaatcttc
     2881 aacatttttc tacttaccgt catctctttg ttatttattg attcatgctt atatggattt
     2941 cgtcttgttg ggaatagcct caaaccaaac gacttggaaa caatgtattg ggtaaaagaa
     3001 aatttacctg aaaattcagt gtttatggtc ttaacgggga ttcccagccc agaaatcgac
     3061 ccatatgtag agtggttccc ggcattagct gaacgtcaga atatctccac attacaaggt
     3121 tatgaatggc tactggcaga taaattttat aatttttata ccgagctttc aaaactacag
     3181 caatgcagat cggttgattg tatagaaaag tggaacacga ccaattcaca acgatacgac
     3241 tatgttgtta tttttagaac agaggcagtc gaaattgatg agctattgtt gaagaactcg
     3301 agttatgaaa aagtctactc aaacagcaac gcattaatcg tcaagtatga cgaatcatct
     3361 acaaaatagc taagatcttt tctattttct tcctaaaaat tcgtgcatac ccttgaggat
     3421 ttgcttccat tccgtctcgg cgttgatttt tcgttgcata tcccccgctc gcttttgcat
     3481 gatctccatc acacgttcgt cttcacccgc atctagccaa aaaccgtgat tattttctgc
     3541 acaggtttct agctttaagt catataaacg atatttaaat tcgtccaata gcataccaca
     3601 gtgcgggcag ggaaactccg tccgtgtttg gaagtgaact agtgaccctt tgtgttcgtc
     3661 ttcatcgaag atcacatctt cgagtttatc aagttcgttg aagtctagcc acatgccacg
     3721 gcagtttggg caggaatcca cttcaaacat tcctttgtag tacttcttcg ttaattcaga
     3781 actacacttt ggacagttca tcgttcttat ccatttcgct tgcagggaag atgctattaa
     3841 ccaaatcctc tgcaaggaca tcattcggtt tcatattgag ccagaccaaa aattcggcat
     3901 ttccttttgg tcccaacaac gaagacttta ccaacccgag caagccaaag ccttctgctt
     3961 ttgcgtagtg taagacatcc aacaaaactt gtt
//
LOCUS       DAOQJE010000227         3970 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_13925_length_3970_cov_5.266922, whole genome shotgun sequence.
ACCESSION   DAOQJE010000227 DAOQJE010000000
VERSION     DAOQJE010000227.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3970)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3970)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3970
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13925_length_3970_cov_5.266922"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..612)
                     /locus_tag="PLT08_07305"
     CDS             complement(<1..612)
                     /locus_tag="PLT08_07305"
                     /inference="COORDINATES: protein motif:HMM:NF013324.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HNE04308.1"
                     /translation="MNISSLLQGIASFAWIGFIGILVTISVRASRKQPVGGLSTAAII
                     LVVTAVLLTTVGAGIVFIEPDELGIVVTVLGSGGIRPDPLDSGLHWIVPFAERVERYP
                     KTNQTYTMTSVADEGQVAGDDSIQVRTKDGQQVYIDASVIYAVDPIKVVQLYSTWRFS
                     YEDQFVRTQARGVIRDVASQYGVEEIVSSKRAEMEQSIDDQLVA"
     gene            685..1359
                     /locus_tag="PLT08_07310"
     CDS             685..1359
                     /locus_tag="PLT08_07310"
                     /inference="COORDINATES: protein motif:HMM:NF021903.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE04309.1"
                     /translation="MKTGTGKIEELFLDGSARISCPPELIPAPGQYLLAHAAGSDAPL
                     PVPLFSSLSSSQNGFRFAPPTPSSWQPGQLLHLRGPIGHGFSLPSSARKIALVGLEDT
                     PARLLGLLSQALKQSAEVVLVSNSSVEDLPEVVEIQPVKGLLDVLRWADYVAMDVNRE
                     KLNQLKEMLAGKDQITAKVEAQVLIRAPMPCGALADCGVCALSIDHDWKMICKDGPVF
                     ELKEIL"
     assembly_gap    1416..1515
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(1755..2867)
                     /gene="wecB"
                     /locus_tag="PLT08_07315"
     CDS             complement(1755..2867)
                     /gene="wecB"
                     /locus_tag="PLT08_07315"
                     /EC_number="5.1.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015703970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008761 - UDP-N-acetylglucosamine
                     2-epimerase activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase
                     (non-hydrolyzing)"
                     /protein_id="HNE04310.1"
                     /translation="MRILTVFGTRPEAVKMAPIVKLLASTPGVEARVCVTAQHRQMLD
                     QVLNLFQIEPHYDLNLMRDGQSLAQLSADIFTHLDPVLEDFKPDWVLGQGDTTTVAIT
                     SMLCFYRQIRFGHVEAGLRTHDKWQPFPEEVNRRIVSVIADLHFAPTNWSKNNLLREG
                     VDEKTVRVTGNTVIDALQFVSKQAEPAEITELVQSKLSHKKLILVTAHRRENFGAPME
                     NICKALLQIASRGDVEIVYPVHLNPNVQEPVSRLLRGVEHVTLLPPLDYLPLVHLMKH
                     ANIILTDSGGIQEEAPAFGIPTLVLRDVTERPEGVDAGTLKLVGTETSHIVEETRRLL
                     EDESAYKKMATSANPYGDGHAAEKIIQALLGSRENM"
     gene            2916..3071
                     /locus_tag="PLT08_07320"
     CDS             2916..3071
                     /locus_tag="PLT08_07320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE04311.1"
                     /translation="MFKLIKFTKRSIILALYFSLILGLASCAKATCGMQAVAGGSQIC
                     IEVLNAE"
     gene            complement(3072..>3970)
                     /locus_tag="PLT08_07325"
     CDS             complement(3072..>3970)
                     /locus_tag="PLT08_07325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003979 - UDP-glucose 6-dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
                     /protein_id="HNE04312.1"
                     /translation="LVAPILEKSKLKAGVDFHLCYSPERVLPGQILRELIENSRVIGG
                     VTPESAKAGADLYSTFVKGQIIQTDATTAEMVKLMENTYRDVNIAIANEFSRLADKFG
                     VDVWEAISIANLHPRVKILSPGPGVGGHCISVDPWFFVEAAPNLTPLIYNSRQVNDAQ
                     PAFVVDVVKRALGSLKGKKIAALGLAYKPDVDDLRESPAVEAVHFFQKEGAEVLACEP
                     FKPDAKLPGVTIAPTFEDAVRDADAIILLVKHTEFSKLKPEQVAQQTKARLLIDTVQG
                     WDAEQWKHAGFRHVKLGSGKHA"
BASE COUNT          968 a          954 c          929 g         1019 t
ORIGIN      
        1 agctactaat tgatcatcga ttgactgttc catttcagcg cgcttcgagc tgacgatttc
       61 ttctacgcca tattgtgatg ccacatcacg gatgacgcca cgtgcctgag tgcgcacaaa
      121 ttggtcttca tagctaaacc gccaagtgga atacaactgc accactttga tcgggtctac
      181 agcgtaaatg acggaagcgt caatataaac ctgctgacca tctttcgtgc ggacctgaat
      241 agagtcatcc ccggcaactt gcccctcatc ggctactgac gtcattgtat acgtctggtt
      301 tgttttgggg taccgctcta cgcgttcagc aaaaggcaca atccaatgca accccgaatc
      361 aagcgggtct gggcgaatac cgccagagcc tagcaccgtc acaacgatac cgagttcatc
      421 tggttcaatg aacacaattc ccgcacccac agtggtcaac aaaaccgctg taaccaccaa
      481 aataatcgca gccgtgctta acccaccaac cggctgtttt ctactagcgc gcactgagat
      541 cgtaactaaa attccaataa aaccgatcca tgcgaaagaa gcaatacctt gcagcaaact
      601 tgatatattc atggttcctc caattgatct cgcaaaatta taactggctt gtcatggcta
      661 ccgaagcatg ttaatatttt tcaaatgaaa actggcacag gaaagatcga agaacttttt
      721 ttggatggct ccgcccggat ctcatgtccg cccgagctga ttcctgcccc tgggcagtac
      781 cttctcgccc atgcagccgg ctcagatgca cccttaccgg ttcctctttt ctcaagcctt
      841 tcctcatctc aaaacggatt tcgcttcgcg ccgccaacac cttcttcttg gcagccaggt
      901 cagttactac atctacgtgg accgatcggg catggtttca gcctgccgtc ttcggcaagg
      961 aaaatagcgc ttgttggctt ggaggatacc cctgcacgat tgctgggact gctttcgcaa
     1021 gcgttgaaac aaagcgccga agttgtattg gtctccaact cgtctgttga agacttgccc
     1081 gaggtggtag aaattcagcc ggttaaaggg ctacttgacg tgcttcgctg ggcagactat
     1141 gttgccatgg atgtaaatag ggagaagctg aaccagctaa aagaaatgct ggcagggaag
     1201 gatcaaatca cggcgaaggt agaggcgcag gttcttatcc gtgcccctat gccttgtggc
     1261 gcactggcag attgcggtgt ctgtgctttg tccatcgatc atgactggaa aatgatttgt
     1321 aaagatggtc cagtgtttga gctgaaagaa atcttataaa aaaagcctcc catttgggag
     1381 gcttttgctt agtaccaaat tctgaaggat gttgtnnnnn nnnnnnnnnn nnnnnnnnnn
     1441 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1501 nnnnnnnnnn nnnnnccgta aaggtaggcg gcgttagggt tgctattgta aaaagcaaga
     1561 aagtctccag agacattatg tcccgtctca ctgaaatatt gagtctctac agactgtgca
     1621 tgaacaggaa tccaagcagc caggagcaga agcgtcagaa caatgggcgt gaaagttttc
     1681 ctcattacta ccaccactgt cattatactc agaaacaccc ctcaagacat tacaaacagt
     1741 tatcttgaga aagattacat gttttcccta ctgcctagta aagcttggat gattttctct
     1801 gcagcgtgtc catctccgta tggattggcg gatgttgcca tctttttgta tgcagattca
     1861 tcctctaaga gccgcctcgt ttcttcgacg atgtggctgg tttcggttcc aacgagcttt
     1921 aatgtccccg catcgactcc ctcagggcgt tcggtcacat cgcgtagtac cagtgttggg
     1981 attccaaagg ctggggcttc ttcctgaatc ccgcctgaat cggtcaatat gatgtttgca
     2041 tgcttcatca aatgaacgag cggaagataa tccagtggcg gtagcagagt aacatgctcc
     2101 acgcctctca atagacggct aacaggttct tggacatttg ggttgagatg aacgggatac
     2161 acgatctcta catcccctcg gcttgcgatt tggagcaatg ctttacaaat attctccatc
     2221 ggcgcgccaa agttctcacg gcgatgcgcc gtgacgagaa tcaacttttt atggctcagt
     2281 ttagattgca ccagttcggt gatttctgct ggttcggctt gctttgacac gaactgcaaa
     2341 gcatcaatga cagtgttccc ggttactcta acggttttct cgtccacacc ttcgcgcaat
     2401 aaattgttct ttgaccaatt cgtgggcgca aaatgcaaat ccgcaatgac gctgacgatg
     2461 cggcggttta cttcctctgg gaaaggctgc cacttgtcat gggtccgcaa accagcttcc
     2521 acatgcccga agcggatttg gcgataaaag cacagcatgg atgtgatggc aactgtggtc
     2581 gtatcgcctt gcccaagtac ccaatctggt ttgaaatctt ctagaacagg gtcgagatgc
     2641 gtgaaaatat ccgcacttaa ttgggcaaga gattgtccat ctcgcataag gttcaaatca
     2701 taatgtggtt caatttggaa caggttcaac acttggtcaa gcatctggcg gtgttgcgcc
     2761 gtaacacaaa cccgagcctc aacacctggc gtagaagcaa gcagcttcac aattggtgcc
     2821 attttgacgg cttcgggtcg agttccaaaa actgtaagga ttctcataag tcctttttgg
     2881 gcctgttttt ctactaggtg tgtggagaaa caaatatgtt caaattaatt aagtttacaa
     2941 aacgttctat aattttggcg ctttatttca gccttattct agggcttgca tcttgtgcaa
     3001 aagccacctg cggtatgcaa gcggttgcag gtggctcgca aatatgtatc gaagttctga
     3061 acgctgagta actatgcgtg ctttccagac ccaagcttga cgtggcggaa acctgcatgc
     3121 ttccattgct ctgcatccca accttggacg gtatcaatca agaggcgtgc ttttgtttgt
     3181 tgagcaactt gttcaggctt gagcttgcta aattcggtat gttttacgag caagataatc
     3241 gcgtcggcat cacgaactgc atcttcaaag gttggcgcaa ttgttacgcc gggcaatttg
     3301 gcatctggtt tgaaaggctc gcaagcgaga acttctgcgc cttctttttg gaagaagtgc
     3361 accgcttcaa cagccgggct ttcacgtaaa tcatccacat caggcttgta tgccaagccg
     3421 agagcggcga tctttttccc tttgagggaa ccaagtgcgc gtttgaccac atctacaaca
     3481 aaggctggtt gcgcatcgtt gacttggcga gagttgtaaa tcagcggagt gagatttggc
     3541 gccgcctcta caaagaacca agggtctacg ctgatacagt gaccaccaac ccctgggccc
     3601 gggctgagaa ttttgacacg tgggtgcaaa ttggcaatgg agatggcttc ccagacatcc
     3661 acaccgaatt tatcggcaag gcgagagaat tcattagcaa tggcaatgtt gacatcgcga
     3721 taagtattct ccatcagttt gaccatttcg gctgtggtcg catcggtttg aatgatctgc
     3781 cctttgacga aggtagagta caagtctgct ccggctttag cagattcagg ggtcacgccg
     3841 ccgataacgc gtgagttttc aatcaactcg cgcagaattt gccccggcaa gacgcgttct
     3901 ggtgaatagc aaaggtgaaa gtccacgccc gctttcagct tggatttttc taaaatgggg
     3961 gcaaccaagt
//
LOCUS       DAOQJE010000228         3966 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_13944_length_3966_cov_5.754538, whole genome shotgun sequence.
ACCESSION   DAOQJE010000228 DAOQJE010000000
VERSION     DAOQJE010000228.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3966)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3966)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3966
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13944_length_3966_cov_5.754538"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..795
                     /locus_tag="PLT08_07330"
     CDS             <1..795
                     /locus_tag="PLT08_07330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HNE04313.1"
                     /translation="MTQVVLAPCSPFSVTSDLMKQSAKLARQYGVHLHTHLAETEDEE
                     QFCMQMFGHRPVGYMQEVDWVGDDVWFAHAVWVNDEEIQVFAKHNCGVAHCPTSNMRL
                     ASGIAPVKEYRKAGVNVGLGVDGSASNDGSHLLAEVRNAMLVSRVKEGLTGYSLSNDP
                     NRKLMTAREALHLGTRGGAAVLGRKDIGSLEPGKCADFFAVDLNKLGYAGMHDPVSAI
                     VFGQSFNVDYTVVGGKFVVKEGQLVTVDQNKLIEKHNKAAKRLLAG"
     gene            823..2613
                     /gene="ade"
                     /locus_tag="PLT08_07335"
     CDS             823..2613
                     /gene="ade"
                     /locus_tag="PLT08_07335"
                     /EC_number="3.5.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000034 - adenine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0006168 - adenine salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine deaminase"
                     /protein_id="HNE04314.1"
                     /translation="MSSSTKLATALVDVAMGRANADLVVKNGKWVSVQSGEIVPNIDI
                     AVVQGHIAFVGRDASHTIGKKTKVIDAKGHYLVPGLLDAHMHVESGMVTVTEFVRAVA
                     VRGTTGMFIDPHEIANVFGLKGVKLMVDEAQKQPIHVWVQMPSCVPSAPGLETPGSSI
                     GPKEVAEAMTWKGIIGLGEMMNFPGVFMSDKKMLDEMSATHAAGKTIGGHYASPDLGL
                     PFHGYVAGGAEDDHEGTRMDDAIQRVRQGMKAMLRYGSAWHDVAAQVKAITEKKLDSR
                     RFLLCTDDSHAETLTTEGHMDRVLRHAISEGLNPMTAIQMMTINTAEHFGLTKEMGMI
                     APGRWADILLVEDLMNFSADLVIAKGQVIAEKGEWQVKLPVVKYPKWVTNSVHLKRKL
                     TATDFILRTRAAHGSEVEAHVIGVIENQAPTRHLKMKVKAENGEVKADIKQDLAKIAL
                     VERHRATGKVTVGLVSGFEFTRNCAIGSTVAHDSHHMIVVGNDDESMAVAANELAKCG
                     GGQIVVMDGKVIGQVEFPIAGLMSNERADVVAKKAATVLEGFKMCGSELNNPNMQLSL
                     MALVVIPELRISDKGLVDVTQFDFIPVLED"
     gene            2629..3345
                     /locus_tag="PLT08_07340"
     CDS             2629..3345
                     /locus_tag="PLT08_07340"
                     /inference="COORDINATES: protein motif:HMM:NF019322.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HNE04315.1"
                     /translation="MANFPFQRLQTDLAALINKTPAGQRLPSEPELAKQLGVSRATLR
                     EAMRSFETQGLIRRRQGSGTFVVGKVQALDSGLEVLESLETLAKRIGLEVSVSDLSIE
                     AIAADADLAASLNVTVGAKLTRVRRVIRAEGRPVAYLVDTLPEDFVHAKDVPSDFHGS
                     VLDFLLNRGDLLASSRANVSAIGATADVAKPLEIQRGDVLLHFNSQLFDSEGKVVDYS
                     LSYFIPGYFRFHIVRRVGNG"
     gene            3399..>3966
                     /locus_tag="PLT08_07345"
     CDS             3399..>3966
                     /locus_tag="PLT08_07345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HNE04316.1"
                     /translation="MTDTTKEIQKRVQSSREDIIEFMRDIVAIPSMDSQIGPVGERIQ
                     AEMKKLGFDEVRFDKMGNTIGRIGNGKRVMVYDSHIDTVGIGDPSSWQWDPFKGKIED
                     GILYARGACDEKGSTPGMIYGLAIARDLGLLDGWTVYYFGNMEEWCDGIAPNTFAEVD
                     PGIKPDFVVIGEPTKMLVYRGHKGRLEMK"
BASE COUNT          956 a          942 c         1127 g          941 t
ORIGIN      
        1 atgactcaag tcgtccttgc gccttgctcg ccgttcagcg tcacttcgga tttgatgaag
       61 caatctgcaa aattggcaag gcaatacggc gtccatctcc acactcattt agccgaaacc
      121 gaagatgaag aacaattctg tatgcagatg ttcggtcatc gtccagtagg ttacatgcaa
      181 gaagtggact gggtgggtga tgatgtctgg tttgcgcacg cggtctgggt caatgacgaa
      241 gagattcaag tctttgccaa acataactgc ggcgtggcgc actgtcccac ctcgaacatg
      301 cgtttggctt cgggcattgc tccggtcaag gaataccgga aagcaggcgt gaacgtcggc
      361 ttaggcgtgg acggttctgc ctccaacgac ggctcacatc tccttgcaga agtgcgaaat
      421 gccatgctcg tctcgcgagt caaagaagga ctgacgggct attccctctc gaacgatccc
      481 aaccgaaaat tgatgactgc ccgcgaagcc ttgcatttgg ggactcgtgg cggtgcagcg
      541 gttttaggac gtaaagatat cggcagtttg gaacccggca aatgcgccga cttcttcgcc
      601 gtggatttga acaaactcgg ctatgctggc atgcacgacc ccgtctctgc cattgtcttc
      661 ggtcaatcct tcaatgtcga ttacaccgta gtgggcggca agttcgtcgt caaagaaggt
      721 caactcgtga cggtagacca aaacaaactc atcgaaaaac ataataaggc tgccaaacgt
      781 ctgttggcag gttaaccctt ttcaacccag aacggagatc ttatgtcatc ttccaccaaa
      841 ctcgccacgg ctcttgttga tgttgccatg gggcgcgcca acgccgacct tgtcgtgaag
      901 aacggcaagt gggtgagcgt gcaatcgggt gagatcgttc cgaatatcga tattgcggtg
      961 gtgcaagggc atattgcctt tgtggggcgc gatgccagcc ataccatcgg caaaaagacc
     1021 aaggtcattg atgccaaggg acattatctc gtgcccggtc tgctcgatgc acatatgcac
     1081 gttgagtcag gcatggttac agtgacggag ttcgtgcgtg ccgtggcggt gcgtggtacc
     1141 accggcatgt tcatcgaccc gcatgagatc gcaaatgtct ttggtctcaa aggcgtgaag
     1201 ttgatggtgg atgaagccca gaagcagcct attcatgtat gggtgcagat gccctcttgt
     1261 gttccgtccg ctccgggatt agaaaccccc ggctcttcaa ttggaccgaa agaagttgcc
     1321 gaagcgatga cctggaaagg catcatcggc ttgggcgaaa tgatgaactt ccccggcgtt
     1381 ttcatgagcg acaagaagat gctcgatgaa atgtccgcga cgcatgcggc gggtaaaacg
     1441 attggtgggc attacgcctc gcctgatctc ggtttgcctt tccacggcta tgtggctggc
     1501 ggcgccgaag atgaccacga aggcacccgc atggacgatg cgattcagcg cgtccgccag
     1561 gggatgaaag ccatgctgcg gtatgggtcc gcgtggcatg atgtggcggc gcaggtcaaa
     1621 gccattaccg aaaagaaatt ggattcacgg cgcttcttgc tctgcaccga tgactcacac
     1681 gctgagacgt taaccaccga aggtcacatg gaccgcgttt tgcggcatgc catcagcgaa
     1741 ggtttgaacc cgatgacggc gatccagatg atgaccatca acaccgctga acatttcggt
     1801 ctcaccaaag agatgggcat gatcgctccg ggcagatggg cagatatttt gctggtggaa
     1861 gacctaatga atttcagcgc cgatctggtg attgccaaag ggcaagtcat tgccgagaag
     1921 ggcgagtggc aggtcaagtt gccggtggtg aaatacccca agtgggtcac caactccgta
     1981 catctaaagc gcaaactcac agcgacagat tttatccttc ggacaagagc cgctcacggg
     2041 tccgaagtag aggcgcatgt gatcggcgtc atcgaaaatc aagcgccgac acgtcatcta
     2101 aagatgaagg tcaaagctga gaatggcgaa gtcaaagccg atatcaaaca agatttggcg
     2161 aagatcgcgc tagtggaacg tcatcgtgcc acaggcaaag tgacggttgg cttggtgagc
     2221 ggatttgaat ttacacggaa ttgcgccatt ggctcaaccg tggcgcatga cagccatcat
     2281 atgatcgtgg tgggcaacga tgatgaaagc atggctgttg ccgcgaatga actggcgaag
     2341 tgtggcggcg gtcagatcgt ggtgatggat ggcaaggtga ttgggcaggt tgagttccca
     2401 attgcaggtt tgatgtcgaa tgaacgtgcc gatgtggtgg caaagaaagc cgcaacggtg
     2461 cttgaaggtt tcaagatgtg cggcagtgaa ttgaataacc ccaatatgca attgagtttg
     2521 atggcattgg tggttatccc tgaattgaga atttctgata agggtctggt ggatgtgacc
     2581 caattcgatt tcattcctgt gttggaagac taagagagag aacaacctat ggctaatttt
     2641 ccttttcaac gattacaaac tgacctcgct gcgttgatca ataaaacacc agcagggcag
     2701 cgcttgcctt ctgaacctga gttggcaaaa caattgggtg tatcgcgtgc caccttgcgc
     2761 gaagcgatgc gctcttttga aacacagggc ttgatccgcc gcagacaagg ttcaggcacc
     2821 tttgtggtgg gtaaagtgca agcattggat agtgggcttg aagtgctgga aagcctcgag
     2881 acccttgcca agcgcattgg gctggaagtt tcggtcagtg acttgagcat tgaagccatc
     2941 gctgccgatg cagatttggc ggcttcgttg aacgtcactg tgggtgccaa gctgacccgt
     3001 gttcgacgtg tcatccgcgc cgaaggacgc ccggttgcgt atctggtgga taccttgcct
     3061 gaggattttg tgcacgccaa agatgtccct tcggatttcc acggctctgt gctggacttt
     3121 ttgctgaatc gcggtgacct gttagcttct tctcgcgcca atgtgagtgc catcggcgcc
     3181 actgcagatg tggcgaagcc cctcgaaatt caacgtggcg acgtgctctt gcacttcaat
     3241 tctcagcttt ttgattctga aggcaaagtg gtcgactact cattgagcta cttcatcccc
     3301 ggttacttcc gtttccatat cgtccggcgg gttgggaacg ggtaattaca tatattcagg
     3361 attctctacg aaccctgtgt aattctaagg agaaaaatat gaccgataca acaaaagaaa
     3421 ttcaaaaaag agttcaatct tctcgcgaag atattatcga attcatgcgc gacattgttg
     3481 caattccatc catggactct cagattggtc ctgtggggga acgcattcaa gcagagatga
     3541 agaaactggg cttcgatgaa gtccgctttg acaagatggg caataccatc gggcgtattg
     3601 gcaacggcaa gcgggtgatg gtttacgact cgcacatcga tacggtgggt attggtgacc
     3661 cgtcatcctg gcaatgggat ccattcaagg gcaagatcga agatggtatt ttgtacgctc
     3721 gtggtgcctg tgatgaaaaa gggtcaaccc ccggaatgat ctacggtctc gccattgctc
     3781 gcgacctcgg tttgctcgat gggtggacgg tctattattt tgggaatatg gaagaatggt
     3841 gtgatggtat cgcacctaat acctttgccg aagtagaccc gggcatcaaa ccagattttg
     3901 ttgtcatcgg tgaaccgacc aagatgctgg tctatcgcgg acacaaaggt cgtttagaga
     3961 tgaagg
//
LOCUS       DAOQJE010000229         3944 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_14066_length_3944_cov_6.489586, whole genome shotgun sequence.
ACCESSION   DAOQJE010000229 DAOQJE010000000
VERSION     DAOQJE010000229.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3944)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3944)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3944
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14066_length_3944_cov_6.489586"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..221)
                     /locus_tag="PLT08_07350"
     CDS             complement(<1..221)
                     /locus_tag="PLT08_07350"
                     /inference="COORDINATES: protein motif:HMM:NF013098.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HNE04317.1"
                     /translation="MKIKLASVSIDDYDKALKFYTEILGFILKRDIPLGNDARWITVV
                     SPDDPNGVELVLEPNASYPAMKALKESLV"
     gene            complement(218..607)
                     /locus_tag="PLT08_07355"
     CDS             complement(218..607)
                     /locus_tag="PLT08_07355"
                     /inference="COORDINATES: protein motif:HMM:NF024242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HNE04318.1"
                     /translation="MATYTQNMLDYSEQIRNVTQYIREHINESLSREVLAEVAGFSVP
                     HFHRVFIATTGESAASYVRRMRLERAGRKLRMGAIDITEVALAAGYVSHAAFGKAFKQ
                     QFGLSPSEFRQLSCSHATKLLTRGMKK"
     gene            789..2753
                     /locus_tag="PLT08_07360"
     CDS             789..2753
                     /locus_tag="PLT08_07360"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HNE04319.1"
                     /translation="MSTNPITQETSLKNRPGLRWTLLGVWGLVVLYTIMVLGAVLSPQ
                     FMMFRDASMARILLGIPALLQTIIDYGVDLLLLFGFSVIAVILIVNRSDDWFAILTSL
                     FLIVFGARITNLANNIALTPGYETQGGLILALGDIGIVLFTMLFPDGKFAPKWMKFLV
                     PLLIITMFGIYLFPNTSFYWVKMRDNGYYFLVTVTWYMVGLGSGIYRYYRSSTLAQKQ
                     QIRWIFIGTMGPFVWFILFQALTYLFPALTNSEVASASFQAISRLAGIFMFLMLPLFV
                     AISIVQARLFDIDMIINRSLVYGILTIGLGLTFGLVFIIASAIFRSMHASDQFFFVAV
                     IFSVAAGAGFQPLRKKLQRFVDRFFYHIQIDYQKTPLDVRAEADTNVEGGITLSSYRN
                     LKLIGHGGMAEVYRATDSTGRGTVAVKILRAALAGDEQFQKRFMREAEIVNRLEHPNI
                     VRVTDFGEERGMYYIVMEMLSGPDLNHLIRQEKRISLSNTVSLLRGIADALDYAHLNG
                     LVHRDIKPSNVMLDSSTIPSRSVLTDFGIAKMLDAHTRITNTAGMLGTFDYMAPEQIQ
                     ASADVDGRADIYALGVMTYQMLTGRLPFERPDTGAILLAHLNAPPPDARELIHDLPRS
                     AARAVQKAMAKDVRDRFASAREFIGALETD"
     gene            2875..3645
                     /locus_tag="PLT08_07365"
     CDS             2875..3645
                     /locus_tag="PLT08_07365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE04320.1"
                     /translation="MSKDITRQVANALSVVLALTVNILASTLPLNGQNTGEISDRFLV
                     YFVPAGYVFAIWGIIYIGWIAFTIYQFQPAQKENPRLQNLGYFFALSGLFNAAWLVCW
                     HYNWFGLSVLVMLTLLSLLVVSYLKLNVGLTPVSTAEKWSVDIPFSVYLGWVSVATVA
                     NISDWLYFVNWNGFGIAPQAWAVIMIAIASVLGVLMTVTRRDSGYAFVLVWSFAGIAV
                     KQAGVPLVANAAWGATLFVFGLAFYSILVRRRRNDLTS"
     gene            complement(3649..>3944)
                     /locus_tag="PLT08_07370"
     CDS             complement(3649..>3944)
                     /locus_tag="PLT08_07370"
                     /inference="COORDINATES: protein motif:HMM:NF024811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="HAD hydrolase-like protein"
                     /protein_id="HNE04321.1"
                     /translation="LDELSKTFPPVFEHIFSSEEAGYYKPHPSIYALPFQRLGIPATS
                     LLHVAGSPTDVLGAKSARLQCVWSNRLGQPYLDVNHKADYELKDLSGLLDILK"
BASE COUNT          939 a          923 c          921 g         1161 t
ORIGIN      
        1 ctaacaagtg attctttaag agctttcatg gcaggataac tagcattcgg ctcaaggact
       61 aactccacgc cattgggatc atctggtgag actacggtaa tccaacgggc atcatttcca
      121 aggggaatat cccgctttaa gataaaacct aaaatctctg tatagaactt tagagccttg
      181 tcatagtcgt caatggatac acttgccaat ttgatcttca ttttttcatt cctctcgtta
      241 atagttttgt ggcatggcta caactcaact gacggaattc actagggctc agaccaaact
      301 gttgcttgaa tgcttttcca aatgctgcat gtgaaacata gcctgctgct aaggcaactt
      361 cggtgatatc tatagcgccc atgcgaagtt ttcgcccggc acgttcgagc cgcatgcggc
      421 ggacgtaact tgctgcactc tctcccgtgg tggcaataaa tacgcggtga aaatgcggca
      481 cagagaaccc tgccacctct gccaatactt cacggctcag ggattcatta atatgctcac
      541 ggatatattg ggtaacgttt cgaatctgct cagaataatc caacatgttt tgagtgtagg
      601 tcgccatgcg gctcaatata tttcagagaa agagggtctc gctactccaa atgtatcatg
      661 aaccttagaa aaaataggca acttttcgcc tgctacagcg taagttgact aaaccccgac
      721 caccttccaa aaataaaaag agtgtgaagc ggttttggag ttatgatttt gaaaatcaac
      781 ggcaacttat gtccacgaac cctatcacac aagaaacctc tctgaaaaat cgtcccggac
      841 tgcgttggac gctgcttggg gtttggggac tggttgtgct ctacactatt atggtgttgg
      901 gggcagtcct ttcgccacag tttatgatgt ttagagatgc gagcatggct cgcattttgc
      961 tcggcatccc cgcgcttttg caaaccatta tcgactacgg cgtagacttg cttcttttat
     1021 ttggattctc ggtgattgct gtgattctga tcgtcaaccg ctcagatgac tggttcgcga
     1081 tcctgacctc ccttttcttg atcgtcttcg gcgctcgtat caccaacctt gcaaacaata
     1141 ttgcgttaac tcccggttac gagacgcagg gcgggctgat cttggcgttg ggggatatcg
     1201 gcattgtgct gtttacgatg cttttcccag atggaaagtt cgctcccaaa tggatgaagt
     1261 ttcttgttcc gcttctgatc attaccatgt ttggaattta tctttttcca aatacatctt
     1321 tctactgggt aaagatgcgt gacaatggct attactttct ggtaactgtc acttggtata
     1381 tggttgggtt aggaagtgga atctatcggt attatcgctc ttctaccttg gcgcagaaac
     1441 agcagattcg ttggatcttt atcggcacga tgggaccatt tgtatggttc atcctgtttc
     1501 aagcgctaac atatctcttc cctgcactga caaattcaga agttgcttct gccagcttcc
     1561 aagccatttc gcgtttggct ggcattttta tgttcttaat gttgccgctt tttgtggcta
     1621 tttccattgt acaagcgcgg ctctttgata ttgatatgat cattaaccgc tcgttggtgt
     1681 atgggattct caccatcggg ctgggactca cctttgggtt ggtcttcatc atcgcctcag
     1741 caatcttccg cagcatgcat gccagtgacc aattcttttt cgtggctgtg atcttttctg
     1801 tggcggcggg cgcgggcttt cagcctttac gtaaaaaact tcagcgcttt gtcgaccgct
     1861 tcttttatca tattcaaatt gactatcaga agactccact tgatgtccgc gctgaggcag
     1921 atacaaatgt agaaggcgga attactttat cttcatatcg taatctaaag ctgattggtc
     1981 acggcgggat ggcagaggtc taccgcgcca cagactcaac cgggcgtgga accgtagcgg
     2041 tcaaaatcct gcgagccgca ttggcgggtg acgaacaatt tcagaaacgg ttcatgcgtg
     2101 aggcggagat cgtcaataga cttgaacatc caaacatcgt gcgagtgacc gattttggcg
     2161 aagaacgtgg aatgtattac attgtgatgg aaatgctttc gggtccagac ctcaaccatc
     2221 tgatccggca agagaagcgc atttctttat ccaacactgt gagcctgttg cgaggaatcg
     2281 ccgatgcgtt ggattatgct cacctcaacg gattggttca ccgtgatatc aagccgtcga
     2341 atgtaatgtt ggattcttcc actatccctt cacgttcggt gttgaccgat tttggcattg
     2401 ccaagatgct ggatgctcat actcgcatta ccaatacagc gggcatgctt ggcacctttg
     2461 attatatggc gccagaacaa attcaagcgt ctgccgatgt agatggacgc gcagatattt
     2521 acgctttggg tgtgatgacc taccaaatgc tgaccgggcg cttgccattc gaacgacccg
     2581 acactggtgc cattttgcta gcgcacttaa atgcaccgcc acccgatgcg cgcgagttga
     2641 ttcacgacct gccacgctcg gcggctcgtg ctgtgcaaaa ggcaatggca aaggatgtgc
     2701 gagaccggtt tgcctctgca agagaattta ttggcgccct cgaaaccgat taaagacttt
     2761 tataaaacat gctccccaag catgtttttc ttttctgccg ctctgatagt atttgacagt
     2821 aagagacata gctgtagtcc tatccatctc agtacatcaa taatggagag agacatgtct
     2881 aaagatataa ctcgtcaagt agccaatgca ctttcagtcg ttcttgcatt aaccgtcaac
     2941 attcttgcca gcactttgcc ccttaacgga cagaacaccg gcgaaatctc agatcgcttt
     3001 cttgtatatt tcgtccctgc cgggtatgtc tttgcaattt ggggcattat ttacattggt
     3061 tggatcgcct tcaccattta tcaatttcaa cccgcacaaa aagaaaatcc acgcttgcaa
     3121 aacctgggct acttctttgc cttaagcgga cttttcaacg cggcatggtt ggtgtgctgg
     3181 cattacaact ggttcgggtt gagcgtattg gtgatgctta ccttactttc tctgcttgtc
     3241 gttagttatc tcaaacttaa tgtagggctt acacctgtca gtactgccga aaaatggagt
     3301 gtggatattc cttttagcgt gtatcttggt tgggtgagcg ttgccactgt tgcaaatatt
     3361 tctgattggc tatattttgt caattggaat ggattcggca ttgcgccgca ggcatgggct
     3421 gtgattatga tcgccattgc gagtgtgctt ggggttctta tgacggtcac acgcagagac
     3481 agtggatacg ccttcgtgtt agtttggtct ttcgctggaa tcgctgttaa acaagcaggt
     3541 gttcctcttg tagcaaatgc tgcttggggc gcaactctct ttgtattcgg gctggctttt
     3601 tattcgatct tagtgcgtcg ccgccgcaat gatttaacct cttgaccctt atttcaaaat
     3661 atccaacaat ccacttaagt cttttaactc ataatcagct ttgtgattaa catccaaata
     3721 cggttgaccg agacgattag accaaacgca ctgtaagcgt gcagattttg ctccgagcac
     3781 atctgttggg gagccggcaa catgcaacag agatgttgcc ggaatgccca agcgttgaaa
     3841 tggcaaggca tagatgctgg gatgtggctt gtaataaccc gcttcttccg acgagaagat
     3901 atgctcaaaa acgggaggaa acgtcttgct caattcatcc aata
//
LOCUS       DAOQJE010000230         3916 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_14219_length_3916_cov_7.925667, whole genome shotgun sequence.
ACCESSION   DAOQJE010000230 DAOQJE010000000
VERSION     DAOQJE010000230.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3916)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3916)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3916
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14219_length_3916_cov_7.925667"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1507)
                     /locus_tag="PLT08_07375"
     CDS             complement(<1..1507)
                     /locus_tag="PLT08_07375"
                     /inference="COORDINATES: protein motif:HMM:NF024180.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIR domain-containing protein"
                     /protein_id="HNE04322.1"
                     /translation="MGQEHIFISHSSKNDDVVKRLREILELEKFELWVDSREFTGGDI
                     LEDTLQEQIKTAKSFIILLSIEALSSEWVQKELKLAQEVAEERKADGYKVISLIMPGV
                     PAGLLKPFFPKEPIHIFLAQGATGPDLDSKMPHILAALGKELPNDWEPPTLIKAAPVE
                     ELILELTDPIIEEKEGIRRATATAVLTYNPADNSRAIESKRYRFTAPLGAVELGEIRW
                     YIEKYYQWPTGVFKTRAEKTEKALPEWGNALYKAALGGESAREPFEAWKRTTGSRRFS
                     VQVDGEPLEGTDDAQAALIREAASDLLSLPWEIMHDGGGFLSQGANAARVRRRLPNRQ
                     DTFTAQADLPIRVLLLSPRPEVDEAGNPVGYLDHRSSALPLIQAVENLGELVQVDILH
                     PPTFPALKDALKKAKEAKNPYDIVHFDGHGVYDRRVGLGALCFEAPQDDKKLGQRLMA
                     LVHAHELAAELRQYGVPLIYLDACQTAQSVTDPKASVAAKLLEEGVGSVVAM"
     gene            1761..2096
                     /locus_tag="PLT08_07380"
     CDS             1761..2096
                     /locus_tag="PLT08_07380"
                     /inference="COORDINATES: protein motif:HMM:NF033788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HNE04323.1"
                     /translation="MRRDVFQAIADPNRRAILSLLARQRMTLNGVADNFRISRPAVSK
                     HIRILKECGLVVVIPQGRERFVEARFDKLNEVTDWVEQYRQLWETRFNRLDDLLEMMK
                     QEKKNALEK"
     gene            2080..2562
                     /locus_tag="PLT08_07385"
     CDS             2080..2562
                     /locus_tag="PLT08_07385"
                     /inference="COORDINATES: protein motif:HMM:NF019928.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HNE04324.1"
                     /translation="MPSKNKVTVTAEPGTQELFIVREFDAPRELVYKAHIDPKLYVQW
                     LGPHGYEMVLETFEPTNGGKYRYVHKDKDGNEYGFHGSFHAMSIENMIQTFEFEGYPG
                     HVSLDCMTLEELPGNRTKATIHSIFQSVSDRDGMIQNGMEKGVSEGYERLDDILKEMK
                     "
     gene            2563..3114
                     /locus_tag="PLT08_07390"
     CDS             2563..3114
                     /locus_tag="PLT08_07390"
                     /inference="COORDINATES: protein motif:HMM:NF019614.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1697 domain-containing protein"
                     /protein_id="HNE04325.1"
                     /translation="MNGYLVLLRGINVGGKNIIKMADLKASFEEMGFSKVVTYIQSGN
                     VVLQSEDKDKAVLTTRIEKGLSKRFNFEAKVVVISQKELAAVVKSAPKGFGEDDAKFR
                     YDVIFLKEPLTPKEAMESVKVREGVDTAHAGKQALYFSRLISRASQSYLTKIIGTPVY
                     QNMTIRNWNTTTKLLALMEGQDG"
     gene            3107..3478
                     /locus_tag="PLT08_07395"
     CDS             3107..3478
                     /locus_tag="PLT08_07395"
                     /inference="COORDINATES: protein motif:HMM:NF020397.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1801 domain-containing protein"
                     /protein_id="HNE04326.1"
                     /translation="MAKTNFQSIDEYIAACPLDSQAYLQEIRKLVRALVPDAKEKISY
                     QIAAFERNGRNIIHFAGWKKHVSLYPVPAGSEAFERQIAKYVDGKGTLKFPLDEPLPI
                     KLIERVVKLHLEANKKKTKGD"
     gene            3480..>3916
                     /locus_tag="PLT08_07400"
     CDS             3480..>3916
                     /locus_tag="PLT08_07400"
                     /inference="COORDINATES: protein motif:HMM:NF018663.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HNE04327.1"
                     /translation="MNKIVPSLWFDTQCEEAMNYYVDTFNGAPYKKEASKIISITRYE
                     KGMEAPGAEQMLGKVITGIFELAGQRYMALDGGPTFNFTEAISFYVECADQKEVDYFW
                     DKLSAVPEAEQCGWCKDKFGLSWQIVPRQLGELMGDPDPERSGR"
BASE COUNT         1024 a          904 c         1035 g          953 t
ORIGIN      
        1 tcatcgccac caccgagccg acgccctcct ccagcaattt ggcagcgacg gaagcttttg
       61 ggtcagtcac cgactgtgcc gtttggcagg catccagata aatgaggggg acaccatact
      121 gccgcagctc ggcggctaat tcatgggcat ggacgagcgc catcagacgc tgccccagtt
      181 ttttgtcatc ctgcggcgct tcaaagcaca acgcgcccag ccccacgcgg cggtcgtaaa
      241 caccatgccc gtcaaaatgg acgatatcgt agggattttt cgcctccttc gcctttttga
      301 gtgcatcttt caaagctgga aacgtgggcg gatgcaggat gtccacttgg acaagttcgc
      361 ccagattttc aactgcctga atgagcggca gagcgctgct gcgatggtcg agatacccga
      421 cgggattgcc cgcttcatcc acttcgggac gcgggctgag caacaacacc cgaatcggga
      481 ggtcggcttg cgctgtaaac gtatcttgtc ggttcggcaa acgacgccgt acccgcgccg
      541 cgtttgcccc ctgcgagaga aacccgcctc catcgtgcat gatctcccac ggcaacgata
      601 gcaggtcact ggcggcttcg cggatcagcg cggcttgggc atcgtccgtg ccttcgagcg
      661 gttcgccatc cacctgcacc gaaaaacgac ggctgccagt tgtgcgcttc caagcctcaa
      721 aaggctcgcg ggcagattcc ccgcccaagg cagccttgta gagtgcgtta ccccattctg
      781 gtagcgcctt ttcagttttc tcggcgcgcg tcttgaacac gcctgtgggc cactgataat
      841 atttctcgat ataccagcga atctcgccca actctacggc tcccaatggc gcagtgaaac
      901 gatagcgttt gctttcgatg gctcgactgt tatcagcagg gttgtaggtc aatacagccg
      961 tggcggttgc gcggcggatg ccttccttct cttcgatgat cgggtcggtc agttcgagaa
     1021 tcagttcttc aacgggtgcg gctttaataa gtgtaggggg ttcccagtca ttgggtaatt
     1081 ctttccccaa cgctgcaagg atatgcggca tcttggaatc caggtcagga cccgtcgctc
     1141 cctgcgccaa aaagatatgg atcggctctt tggggaagaa gggtttcaat aagcctgcag
     1201 gcacgcctgg catgatcaag gagatgactt tatacccatc cgccttgcgt tcttcggcaa
     1261 cttcctgcgc caatttcaac tctttttgca cccactctga actaagcgct tcaatactca
     1321 aaaggataat gaaagatttg gcggttttta tctgctcttg cagggtatct tcgagaatat
     1381 ccccgcctgt gaattcacgc gaatccaccc aaagctcaaa tttctcgagt tcgagaattt
     1441 cgcgcaatcg tttgacgaca tcatcgtttt ttgaagagtg cgagataaag atatgttcct
     1501 gtcccatcga attgcctcct gcgagaaatt catcaatgtt ccacttggtt aagcttggct
     1561 aacaggtaca aaattagagt tatgcagctt ccatagtccc ttattggaaa attatacagc
     1621 taaagatttg gataagtata aacagactca aatggcaata tggcaaaagt gtaacatacc
     1681 gatctaaacc ttgacaaaat agaaattatg ttctaaaata atttcgcaat aagtaaccgt
     1741 ttggttacat aaaggagacc atgagacgag acgtatttca agctattgca gaccctaacc
     1801 gtagagcgat cctctctttg ctggcaaggc aacggatgac attgaacggt gttgccgaca
     1861 attttcgtat cagtcgtcct gccgtttcga aacacatcag gatattgaaa gagtgcggac
     1921 tggtggttgt catcccacag ggacgtgagc gttttgtgga agcacggttc gacaagctaa
     1981 atgaagtgac cgattgggtc gaacaatatc gccaattatg ggaaacgcgc ttcaatcgat
     2041 tggatgacct tttagaaatg atgaaacagg agaagaaaaa tgccctcgaa aaataaagtg
     2101 accgttaccg ctgaacctgg aacacaggag ctttttatcg tccgtgaatt cgatgcgccg
     2161 cgtgaattgg tgtacaaagc ccacattgat ccaaaactgt atgtgcagtg gcttggtccg
     2221 catggatatg agatggttct cgaaacgttt gagcccacca atggtggaaa gtatcgctat
     2281 gttcacaagg ataaggatgg aaacgaatac ggtttccatg ggtcgttcca tgcgatgtcc
     2341 attgagaaca tgatccagac cttcgagttc gagggatatc cggggcacgt ttcgctggat
     2401 tgtatgacgc tggaggaatt gcccgggaat cggacgaaag ccacgattca ttccatcttt
     2461 caatctgtct ctgaccgaga cggcatgatc caaaacggaa tggaaaaagg tgtgagtgaa
     2521 gggtatgaac ggctcgatga tattttgaag gaaatgaagt aaatgaatgg atatctggtt
     2581 cttctgcgcg ggatcaatgt gggcggaaag aacatcatca aaatggccga tttaaaagcc
     2641 agttttgagg agatgggctt ttcgaaggtg gttacctaca ttcaaagcgg aaatgtcgtc
     2701 cttcagtcgg aagataagga taaagctgtg ctgaccacca gaatcgaaaa gggactttcg
     2761 aagcgcttca actttgaggc gaaggtggtg gtgatttctc aaaaagaact ggctgctgtt
     2821 gtgaaatctg cgccgaaggg atttggcgag gatgacgcga agtttcgcta tgatgtcatc
     2881 tttttgaagg agccgcttac gccgaaggaa gcgatggaga gtgtgaaagt ccgcgagggc
     2941 gtggatacgg ctcatgcagg caaacaggcg ctgtattttt cacgtctcat cagccgcgct
     3001 tcgcagagtt atctcaccaa gatcattggc acgcctgttt atcagaatat gaccattcgc
     3061 aattggaata caactacgaa actattggca cttatggaag gacaagatgg ctaagacaaa
     3121 ttttcaatcc attgatgaat atattgcggc atgcccgctg gattcgcagg catatttaca
     3181 agagatccgc aaactggtcc gcgcactggt gccggatgcc aaagagaaga tcagctatca
     3241 gatcgcggcg tttgaaagga acggcaggaa catcatccat tttgcggggt ggaaaaagca
     3301 tgtgtcgctg tacccggttc cggcggggag tgaggcgttc gagcgtcaga tcgcaaaata
     3361 tgtggatggc aaaggcacgt tgaaatttcc gcttgatgag ccgcttccaa taaagttaat
     3421 tgagagagtc gtcaaattgc atttagaggc aaataagaag aaaaccaaag gagattgaaa
     3481 tgaataagat tgttccgagt ttatggttcg atacacaatg tgaagaggca atgaactatt
     3541 acgttgatac gttcaacggt gcgccataca aaaaggaagc ttcgaagatc atttccatta
     3601 cgcgctatga aaaaggtatg gaagcacccg gcgcagaaca gatgttaggc aaggtcatta
     3661 ccggtatttt tgagttggca ggtcaacgct atatggcgct cgatggcgga ccaaccttta
     3721 acttcaccga agcaatctcg ttctatgtgg aatgtgcaga ccagaaagag gtggattatt
     3781 tctgggataa gttatctgct gttcctgaag ctgagcaatg cggctggtgc aaggataagt
     3841 tcggtttgtc ttggcagatc gtcccaaggc agttgggcga gttgatgggg gacccagacc
     3901 ctgaaagatc aggacg
//
LOCUS       DAOQJE010000231         3914 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_14230_length_3914_cov_4.479399, whole genome shotgun sequence.
ACCESSION   DAOQJE010000231 DAOQJE010000000
VERSION     DAOQJE010000231.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3914)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3914)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3914
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14230_length_3914_cov_4.479399"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..931)
                     /locus_tag="PLT08_07405"
     CDS             complement(<1..931)
                     /locus_tag="PLT08_07405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamate-ammonia ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase beta-grasp
                     domain-containing protein"
                     /protein_id="HNE04328.1"
                     /translation="MAKSVKEVLELAKGVQVVDLRFTDLPGTWQHFTMPVQRLNEDFF
                     KEGIPFDGSSIRGFQEIHESDMLLKADPETAFVDPTAETPTLVLSCNVFDPITEVAYS
                     RDPRYVAQKAEAYAKSTGLADTVYFAPEAEFFVFDGVRYTSTPNESGFSIDSEEGWWS
                     SNREGAKGGQIQPKRGYFPVSPTDTLQELRNKFMLAMQAAGITMDLHHHEVATAGQSE
                     MSMTFTTLTRMADDLLLYKYIIKNMARKYGKTVTFMPKPIFGDNGSGMHVHSSMWKDG
                     KNVFYDEKGYAGLSQTAKYYIGGLLKHAPALLAL"
     gene            complement(1501..2835)
                     /gene="glnA"
                     /locus_tag="PLT08_07410"
     CDS             complement(1501..2835)
                     /gene="glnA"
                     /locus_tag="PLT08_07410"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamate-ammonia ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glutamate--ammonia ligase"
                     /protein_id="HNE04329.1"
                     /translation="MTSDQISDILARTEQNQIKFIDLQFTDVVGVVKNVTIPIHQLPA
                     ALENGIWFDGSSIEGFARIAESDMHLRPDPSTLAIIPWLKGEEATARLICNVYTPDGQ
                     PFPGDPRSILRKMLDEAADMGFRYNTGPELEFFLLKPNANGSLTPPTPHDSGSYFDAP
                     SDMSAGLRRQMANTLDALGLQVEAMHHEGAHGQQEIDFRYSDALTTADSAVTLRVALK
                     VIAQLNGLYCTFLPKPIRGINGSGMHVHQSLTYVANGKNAFSDPGDPHGLSKIAKHFI
                     AGQLAHAKGMCAILAPLVNSYKRLVAGYEAPVHISWARINRSALIRVPRASESDSTRI
                     ELRCPDPSCNPYLAFTVMLAAGLDGIRRELQVPEATEENIYLLEKPNYSSYPLLPTSL
                     NQALRALEEDEVVRNALGAHTHEVFISAKRLEWEDYRLEVTDWELEKYLPNY"
     gene            complement(2857..>3914)
                     /locus_tag="PLT08_07415"
     CDS             complement(2857..>3914)
                     /locus_tag="PLT08_07415"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF019334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HNE04330.1"
                     /translation="PRRIAWVVQETKENSVMNAYAIITNTMLQIGDQFDHYQIQAHMA
                     QGGMSDVYRAFDLMHRREVVIKVPDHSMIGDPAQFERFQRELEVMKSLSHPAILKGLD
                     SGKHNRVPYLVTEFVHGESLRTLMEKSGSLPTDQVVSLIQKLANGMAYCHKNEVIHRD
                     LKPENILITSDGQPVIMDFGLALTKDSHRVTYSNLSATMGTPDYMSPEQIEGQRGDQR
                     SDIYALGTIMYEMLAGHTPFTGDTPMAVMAQHLNNNAPRLDKVNPSISVHLAAIVARC
                     LARNPEDRYPDMDSLIAVLDTPETVDISILDKSSAPPKSSSLSLTAQQTIKGVLIAAG
                     IIILLILLSLGLQTLRP"
BASE COUNT          966 a          834 c         1095 g         1019 t
ORIGIN      
        1 tgagtgcgag gagcgcagga gcgtgcttga gcaaaccacc gatgtagtac ttggctgtct
       61 gggaaagacc cgcgtaaccc ttctcatcat agaagacgtt cttgccgtct ttccacatgg
      121 aggagtggac gtgcatacca gaaccgttgt caccgaagat gggcttgggc atgaaggtta
      181 cggtcttgcc gtacttgcga gccatgttct tgatgatgta cttgtaaagc aggaggtcat
      241 ctgccatgcg ggtgagcgtg gtgaaggtca tgctcatttc gctctgacca gcggtggcaa
      301 cttcgtggtg gtgtaagtcc atggtgatgc cggcggcttg catggcaagc atgaacttat
      361 tgcgtaattc ctgaagggtg tcggtggggg aaaccgggaa gtaaccgcgc ttcggctgga
      421 tctgaccgcc tttggcacct tctcggttgc tgctccacca gccttcttca gagtcgatgg
      481 agaaaccaga ctcattgggg gtggaggtgt agcgaacgcc atcgaagacg aagaattccg
      541 cttcaggcgc gaagtaaacg gtgtcggcga gacccgtgct cttggcatac gcttcagcct
      601 tctgtgctac atagcgcgga tcgcgagaat aggctacttc ggtgatcggg tcaaagacgt
      661 tgcaggataa gacgagggta ggggtttcgg cggtgggatc tacaaacgca gtttcggggt
      721 cagctttgag gagcatgtcc gattcgtgaa tttcctggaa gccgcggatg gacgaaccat
      781 cgaaaggaat accttccttg aaaaagtcct cattgaggcg ctgaacaggc atggtgaagt
      841 gctgccaagt gccgggcagg tcggtgaagc gcaggtctac aacctgaacg cccttagcca
      901 gttccaaaac ttctttgact gatttagcca ttcttttctc tcctaacaaa ttttgttgtt
      961 ttcctctgtt gagtagcctt aagactacgg aggatatata tttgatatcg gcattatagg
     1021 aggggcgggg agggcgggtc tattacccaa acgggtacac ttgaattgca taaatggcga
     1081 aaaatgactt gtcactcatg gaatgggtac ttaaacgaaa aagggacagg tcctgtgcta
     1141 acctgtccct tcctgtccct gttgggacca aaccaaggag atggggaaga acgatgttga
     1201 ttgtagtttc gatatatgac taagtctatt gcccaaatgg gtatatttcg atgagcactt
     1261 aaagaaaaat gggcaggtcc tgtgacacct gtccattttc ttgtccctta taggacgaaa
     1321 ccaaggaggg gggatttgtg aggaaaacta agggataaga aaatcgaatt gaagatccac
     1381 tataaaattt tcatcactat acagaagttg agaggtttaa tccatacccc gaatgggtgc
     1441 tgttctaaac actaaagcgg atacaacgta tccgctttag tgtttggcat tatatcaata
     1501 tcaataattg ggtaaatatt tttcaagttc ccaatcggta acttccaatc gatagtcttc
     1561 ccactcaagg cgtttggcgc tgataaatac ttcatgggtg tgtgcgccca aggcattgcg
     1621 cactacttca tcttcttcta atgcacgtaa ggcttgattg agtgaagtgg gtaacaaagg
     1681 ataagaagaa tagttgggtt tttctaaaag atagatattt tcttccgtcg cttcaggaac
     1741 ttgtaactcg cggcggatgc catccaaacc ggcggcgagc atgacggtaa aagcaaggta
     1801 tggattgcaa cttgggtctg ggcaacgcag ttcaatgcgg gtcgagtcgc tttcggaggc
     1861 gcgcggaacg cggatcaatg ccgagcggtt gatgcgtgcc caactaatat gaaccggtgc
     1921 ttcatatccc gctacgaggc gtttgtatga attgactagg ggggcaagaa tggcacacat
     1981 ccctttggcg tgagcaagtt gaccggctat aaagtgtttg gcaatcttgg aaagtccgtg
     2041 cgggtcgccg gggtctgaga acgcattttt gccattggcg acatatgtga ggctttgatg
     2101 aacgtgcata ccgctgccat taatgccgcg aatgggcttg ggtagaaagg tgcaatatag
     2161 cccattcaac tgagcgatga ctttcaatgc cacacgcaat gtcacggcac tgtcggcggt
     2221 ggttaatgca tcagagtaac ggaagtcaat ctcttgctgt ccgtgtgcgc cttcgtggtg
     2281 catcgcttcc acttgcaagc cgagagcatc taatgtgttt gccatttggc gcctcaaccc
     2341 agcggacata tcggaagggg cgtcgaagta actgccgctg tcgtgcgggg taggcggagt
     2401 taagctccca ttggcgttgg gcttaagcag gaagaattca agttcgggtc cggtgttgta
     2461 gcgaaagccc atgtctgcgg cttcatccag catttttcgc aagatcgagc gtgggtcacc
     2521 gggaaagggt tgaccatcag gagtgtatac attgcagatg aggcgggcgg ttgcttcttc
     2581 tcctttgagc caggggatga tcgctaaagt agaagggtcg gggcgtagat gcatgtcact
     2641 ttctgcaatg cgggcgaagc cttcaataga agaaccgtcg aaccagatgc cattttcaag
     2701 tgcagccggc agttggtgaa tgggaatggt gacatttttg acgacaccga ccacgtcggt
     2761 aaattgcagg tcgataaact tgatctggtt ttgttcagta cgcgccagaa tatctgagat
     2821 ttgatcggat gtcatggaga cgctcacagg ggaagattat gggcgcagtg tttgcaaacc
     2881 cagagacagt aatatcaata agatgatgat gccagctgca atcaaaaccc ctttgatggt
     2941 ttgctgggca gttagagata agcttgagct tttgggtggt gcgctagatt tatcgagaat
     3001 agaaatgtca acagtttctg gggtgtcgag aactgcaatt aatgaatcca tgtctgggta
     3061 gcggtcttca gggttgcgcg ccaaacatct agcaacgatg gcggcaaggt gtactgaaat
     3121 ggaaggattt actttatcca aacgaggcgc attattgttc aggtgttgtg ccatcactgc
     3181 catgggggta tcgcctgtga agggagtgtg cccggcaagc atttcataca tgatcgtacc
     3241 tagggcataa atgtcactgc gttgatcacc tcgttgtcct tcgatttgtt caggcgacat
     3301 atagtctggg gtgcccatgg tggcgcttag gttggagtag gtgacacggt gcgagtcttt
     3361 tgtcaacgcg agaccaaaat ccatgatgac gggttgtcca tcactagtga taaggatatt
     3421 ttcaggtttc aggtcacgat gaatgacttc gttcttgtgg cagtatgcca tcccgttggc
     3481 taatttttgg ataagagaaa caacttgatc ggtcggtaaa gagccgcttt tttccatcaa
     3541 tgtgcgcaat gattcaccat ggacgaattc tgtcacaaga tatgggacgc ggttgtgctt
     3601 cccagaatcc agccctttta gaatcgctgg gtggctaagt gacttcataa cttcaagctc
     3661 acgttggaag cgttcaaatt gggcagggtc gccgatcatg gaatgatcgg gaactttaat
     3721 aacaacctcg cgacggtgca taagatcaaa ggcgcgatat acatcactca ttccgccctg
     3781 agccatgtgt gcctgaattt gataatgatc aaattgatcg ccaatttgca acatagtgtt
     3841 cgtgattata gcataggcgt tcatgacact attttcctta gtttcttgaa ccacccaagc
     3901 gattcttcta ggag
//
LOCUS       DAOQJE010000232         3913 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_14234_length_3913_cov_7.417833, whole genome shotgun sequence.
ACCESSION   DAOQJE010000232 DAOQJE010000000
VERSION     DAOQJE010000232.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3913)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3913)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3913
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14234_length_3913_cov_7.417833"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..506)
                     /locus_tag="PLT08_07420"
     CDS             complement(<1..506)
                     /locus_tag="PLT08_07420"
                     /inference="COORDINATES: protein motif:HMM:NF012869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter transmembrane domain-containing
                     protein"
                     /protein_id="HNE04331.1"
                     /translation="MHGYGWFMAMRQGDEKPKVTRELLLRVLNYAKPYWWHIAGMLVT
                     ILLSTGLSLLTPLIFRNMIDVVLPAKDTQRLIWLGLALLFIPALSGGVNVLQRRLNST
                     VGEGVIYDLRSSLFARLQRMSLRFFTNTKTGELMSRLNSDVVGAQNAISNTIVNIVTN
                     LIEAVALFA"
     gene            778..1551
                     /locus_tag="PLT08_07425"
     CDS             778..1551
                     /locus_tag="PLT08_07425"
                     /inference="COORDINATES: protein motif:HMM:NF012420.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase family protein"
                     /protein_id="HNE04332.1"
                     /translation="MKLYKPTLVLVLAALLVACAPATEEASHASTPHWTYEGEEGPEH
                     WGDLDPSYAACETGTKQSPIDVSNPAEQDLANIAFHYQPSEVNILNNGHTVQVNYDAG
                     SYIELDGVRYDVAQFHYHAPSEHAVDGKLFAAELHIVHKNADGGLAVVGILLDEGAEN
                     AAYQPFIQNLPAEESDVKDAGVTVNAADLLPAVQTTFRYSGSLTTPPCTEGVTWLLMT
                     TPVEISASQIEALASLFEAGNNRPVQPLNDRPLAEDSTP"
     gene            1626..2552
                     /locus_tag="PLT08_07430"
     CDS             1626..2552
                     /locus_tag="PLT08_07430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE04333.1"
                     /translation="MPLRFPVTSLVLIWMLVGCSSLPFVATPTPTALPTSTPTRTPLP
                     TQTPSPTVTPILVTYPFKHVWFKYTVERPAEEANPLRLTEEPLLVIYTDGTMIAARAG
                     DPKVRTRVLNTKEVCDFLNRLDTFGFYTLEDSHSTDETNPLYDFGTKYQAVDEGDFAS
                     LTVNRTDPKSVTFFEPYRKFFARPMRKIMEFVETYNPGALKAYEPDRLVVFVQRGRAE
                     GTDEKVRVRDWTVNTFSLKDATDLPYTFLEGQAAVDVYALLAKYETHIYLENEIEYTV
                     TMRLLFPHEVLNADSTPIAIPTEARLPVNCTP"
     gene            2572..2919
                     /locus_tag="PLT08_07435"
     CDS             2572..2919
                     /locus_tag="PLT08_07435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE04334.1"
                     /translation="MPTKTPSIISTVLTVLSLLVFVIASLFFSLVALNGVSESQGTPA
                     LIASLICNGIGIILAAVVAWKMPAWLIHKFSWKSLWAVLVSLIVSFFLGGGISIVAMF
                     VSILVADILWNAR"
     gene            3066..>3913
                     /gene="glgP"
                     /locus_tag="PLT08_07440"
     CDS             3066..>3913
                     /gene="glgP"
                     /locus_tag="PLT08_07440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucan family phosphorylase"
                     /protein_id="HNE04335.1"
                     /translation="MEFLTSPSQKFDLPKRIERLGELATNLWWSWHPEAAHLFGRLDY
                     DLWERLGHNPIRILNEVGRTRLNQASKEKEYLELYDAVFKTFDAYFSKETWSQKTHKE
                     LKKPIAYFSMEYGLHETLPIYSGGLGVLSGDHLKEASDLGLPFIAVGFMYAQGYFSQR
                     ITEDGWQEALNNPLNFDHLPVSLVTKDGKAVTVSFDFPDRKLTAQIWEVRVGRVPLYL
                     LDSNVESNSEYDRLLTARLYWSDLDRRIMQEVLLGIGGARAIRALGYDPAVWHMNEGH
                     ASFLTL"
BASE COUNT         1012 a          989 c          924 g          988 t
ORIGIN      
        1 gcgaataaag caacagcttc aataagattg gtgacgatgt tgacaatggt attgctaatg
       61 gcgttctgcg ctccaaccac gtcactgttg aggcgagaca tgagttcgcc tgttttggtg
      121 ttggtaaaga aacgtaaaga catgcgctgc aatcgagcga agagcgaaga acgcaagtca
      181 taaattacgc cttcgcctac cgtcgagtta agtctgcgct gcaagacgtt aactccaccg
      241 cttagtgccg ggatgaacag caacgccagc cccagccaaa taagtcgttg ggtatctttg
      301 gcgggcaaga ctacatcgat catgttacga aagatgagtg gggtcagcaa cgataaacct
      361 gtgctgagca aaatagtaac aagcatgcct gcaatgtgcc accaataagg tttggcgtaa
      421 ttgagaacgc gtaataaaag ctcgcgggtg actttgggtt tttcgtcgcc ttggcgcatt
      481 gccatgaacc agccgtatcc gtgcataaag gttgtctccg ataattaggt gtaggtgcga
      541 ctcgtcaaga tcttggtgag gtgatggcgc gtcatgtatc cttgcatgaa aattggcttg
      601 tcacacccag gcgggtcgcc cctacggaat gatgtgacta taagaccaag tgaccattcc
      661 ggcaactgga caaaggggga ggttaatgaa agtctgatga agtgtaaagc aggtgcaaag
      721 aaccttttca aacaaaccaa tacaatacat caaattaaat aggagagagt taatcaaatg
      781 aagttatata aacccacact cgtacttgta ctagctgcct tacttgttgc ctgcgcacct
      841 gcgacagaag aagcctcaca cgcttccact ccacactgga cgtatgaagg cgaagaaggt
      901 cctgaacatt ggggcgacct tgacccatcg tatgcagcct gcgaaacagg caccaagcaa
      961 tcaccgatcg atgtttccaa cccagctgaa caggatttgg cgaatatcgc ttttcattat
     1021 cagccaagcg aagtcaatat tctgaacaat ggacataccg tccaagtcaa ctatgatgcg
     1081 ggcagttaca ttgaattaga tggtgtccgc tatgatgtgg ctcaattcca ttaccacgcg
     1141 cccagtgaac acgctgtgga cgggaaactc ttcgcagcag aattgcatat cgtccacaag
     1201 aatgcagatg gcggcttagc agtggtaggt attttattgg acgaaggcgc agaaaatgca
     1261 gcctatcaac cgttcattca aaacctccca gcagaagaaa gtgacgtgaa agatgcaggc
     1321 gttaccgtca acgccgcaga tttgctccct gctgtgcaaa cgactttccg ttatagcggc
     1381 tcgcttacca caccgccttg tactgaaggt gtgacctggt tgcttatgac tacgcctgta
     1441 gaaatctctg caagccagat cgaagccctt gcttctttat ttgaagcagg caataacaga
     1501 cccgtccaac ccttgaacga ccgtccgctg gcggaagata gtacaccgta acaagaattc
     1561 aagagacagt cacctttgag gtgactgtct cttttaaggg cgggatataa ttcaagacaa
     1621 atttcatgcc tctacgattt cctgtgacct cgcttgtttt gatatggatg ttggttggct
     1681 gtagttcttt gccttttgtt gccacgccca cacctacagc gcttcccacc tctaccccaa
     1741 cgcggactcc actccctacg cagacgcctt cgcctacggt tacacctatt ctcgtcacgt
     1801 atccgttcaa gcatgtgtgg tttaagtaca cagtggagcg cccggctgag gaggcgaatc
     1861 cgcttagatt gacggaagag ccgctgctcg tcatctatac cgatggcacc atgatcgcag
     1921 cgcgggcggg ggatccgaaa gtccgcacgc gggttctcaa cacgaaagaa gtctgcgact
     1981 ttctcaaccg gctagatacc ttcggttttt acacgctgga agattcacac tcgacagatg
     2041 agacaaatcc tttatatgat tttggcacaa aatatcaagc cgtggatgaa ggtgattttg
     2101 ccagcctcac tgtgaatcgg acggacccca aaagcgtaac tttctttgag ccttatcgaa
     2161 agttttttgc aagacccatg cgcaagatca tggaatttgt agaaacatac aatcctggcg
     2221 cgctcaaggc atatgagcca gaccgtttgg tcgttttcgt gcaacgcgga cgagccgaag
     2281 gcaccgatga aaaagtccgc gtgcgtgatt ggacggtgaa tacgttcagt ttgaaggatg
     2341 caacagacct accatataca tttcttgaag ggcaagccgc cgtagatgtg tatgcattgc
     2401 tggcgaagta tgaaacccac atctatcttg agaatgaaat agaatacaca gtcacgatgc
     2461 gtttgctgtt cccacatgaa gtgcttaatg cagactctac gccgattgcc atccccaccg
     2521 aagcgcgcct tcctgtgaat tgcacaccat aacctttatt tgaggaaacc catgcctacc
     2581 aaaaccccgt ccattatttc tactgtgcta acggttctaa gcttgcttgt gtttgtgatc
     2641 gccagcttgt tcttttcttt ggttgctctg aatggggttt cagaaagtca gggcaccccc
     2701 gcgctgatcg catcgttgat atgtaatggg atcggaatca tcctcgccgc cgtcgtcgca
     2761 tggaaaatgc ctgcctggct gattcataaa tttagctgga agagcctttg ggcagtgctg
     2821 gtttccttaa tagtcagttt cttccttggc ggagggattt ctattgttgc catgttcgtc
     2881 agcattctcg tagcggacat attatggaat gcccgttaaa cctgaatcaa cattttgctc
     2941 aaatgacggt catctttaca ggtgaccgtc atttttaatt ccgaagttca tttagtttga
     3001 ccaaacaact aatccgcctt acaatcgctg gacgcaaaaa aacaatgagg tatcatccgc
     3061 ccactatgga attcttaact tctccctctc aaaaattcga tttgccaaaa cgtatcgaac
     3121 gtcttggtga acttgccacc aacttgtggt ggtcctggca tcctgaagcc gctcatctct
     3181 ttggtcgttt ggactatgac ctgtgggaac gactcgggca taaccccatc cgcattctta
     3241 atgaagttgg gcgcacacgc ttaaatcagg catctaaaga aaaagaatat ctcgaactat
     3301 atgatgccgt cttcaaaact ttcgatgcat actttagcaa agaaacctgg tcacaaaaga
     3361 cgcacaaaga gttgaaaaaa ccgattgcct atttctcgat ggaatatggc ttgcatgaaa
     3421 ccctgcccat ttattctggt ggcttgggcg tgctttcagg ggatcatctc aaagaagcct
     3481 cagacctcgg cttgcctttc atcgcagtcg gcttcatgta tgctcagggc tatttctctc
     3541 aacgcatcac tgaagacggc tggcaggaag ctctcaacaa cccgctcaac tttgatcacc
     3601 tgcccgtctc cctcgtcacc aaagatggca aagccgtcac cgtttctttc gattttcctg
     3661 atcgaaagct caccgcgcaa atttgggaag tgcgcgttgg gcgtgttccg ctctacttgc
     3721 tcgactcaaa cgtcgaaagc aacagcgaat atgaccgtct gctgaccgcc cgcctctact
     3781 ggtctgacct ggatcgccgc atcatgcagg aagttctgtt gggaatcggc ggtgcgcgcg
     3841 ccatccgcgc cttgggatat gaccccgccg tctggcacat gaatgaaggt cacgcctctt
     3901 tcttaaccct cga
//
LOCUS       DAOQJE010000233         3907 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_14278_length_3907_cov_4.223001, whole genome shotgun sequence.
ACCESSION   DAOQJE010000233 DAOQJE010000000
VERSION     DAOQJE010000233.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3907)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3907)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3907
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14278_length_3907_cov_4.223001"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..796)
                     /locus_tag="PLT08_07445"
     CDS             complement(<1..796)
                     /locus_tag="PLT08_07445"
                     /inference="COORDINATES: protein motif:HMM:NF020036.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HNE04336.1"
                     /translation="MKFIGDFFKPPVFEDEQKTHQAYLLNIILWGLIAIPIPYVIYAQ
                     IFLSGTSTRALTQSIIGEFINIFLLILLKRGYVRTVSLLQVIAFWIFFTVSAATGIGV
                     QGESYLLGYPLVIMISGILLGNRFTLGLTALSLVSGLLMVFSEGNGLINKDVIRPPFL
                     TWILSFAIFPMSAVLQYLSSRTVNNALKRAKESEEKYRLISSVSSDYAFESRINDNEL
                     GEAIWIAGAFEKMTGYTPDEYIKAGGWYAHIHPDDLDQDMEDMKKLL"
     gene            complement(804..2690)
                     /gene="selB"
                     /locus_tag="PLT08_07450"
     CDS             complement(804..2690)
                     /gene="selB"
                     /locus_tag="PLT08_07450"
                     /inference="COORDINATES: protein motif:HMM:TIGR00475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0001514 - selenocysteine incorporation
                     [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="selenocysteine-specific translation elongation
                     factor"
                     /protein_id="HNE04337.1"
                     /translation="MRVIGTAGHVDHGKSTLIETLTGTHPDRLKEEKAREMTIELGFG
                     WLTLPNGEELGIVDVPGHRDFIENMLSGIGGIDAVLLVISADEGIMPQTKEHLAILDL
                     LQIPAGLIVLTKTDLASDSGWLDLVEADIRHAVSGTVLKEAPILRVSARAGTGLKELT
                     HALETLLQEKPARPDLSRPRLPIDRIFTMSGFGTVVTGTLRDGHLSVGDEVEILPSGL
                     QGRIRGLQTHKKKEESAVPGSRTAVNVSGVDVEQIRRGDVLAHPGQYQSTRRIDVRFK
                     ALPDISKPLTYGDEVKFFVGASETVAIARLLGTEELKAGEEGWLQLELRDPIVALRGD
                     RYILRRPSPGETLGGGAVVDHQPKGRHKRFDSDVLKSLESLAQGSPADVLFEAALALQ
                     IASIKDMVSKSRLEVKVAESALNELLQNGQLISLENSLKPEALAVAFPYWSKLSTTIL
                     DTVNGYHSQYPLRRGIPREELKSKLKLTPKVFNAAIAFLASKSDLVDERGAVLKPGHE
                     IKFDGQNQVKVQVLRRKFESNPYATPSVKECQAEADEEIVNALIETGELVLVSQDVIF
                     RKVDYDEMVAKIKNTLQSQGQITLAEVRDMFNTTRKYVQALLEHLDATGVTMRDGDAR
                     KLRK"
     gene            complement(2769..>3907)
                     /locus_tag="PLT08_07455"
     CDS             complement(2769..>3907)
                     /locus_tag="PLT08_07455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE04338.1"
                     /translation="ETKETLTSLKESLASFETTVNAVSKQIKTLSKQVTDITPAAKRV
                     DQFETMVGKQRNELVKMIEDVDKAHTRSERDTTKQFQSDINELSKTLTQLKVSTDLTE
                     IKKQLKERGNEIQRALANIADFKTVVEDTKRSNEDVKLSIKAADEARKNDLKRIADLQ
                     GELTALRKRIDENREKSTIQGDTIRNIETRITELLSSELERKQAQAVFLDQQRVAQVD
                     RERAWKDWQDKFVSFQKEAETLDQQIQRLDETLRNAKKAQDTYMELNSKLERRINEVT
                     EMQRLAEERLRQEWISFKADDQKRWTGYSLSSEESFRDIRKEVSKYEGSISTLNEVSQ
                     VLQDQLHQTTDASEKQLQEMMNVVHEWMTSYQRIMGHGKKPVKK"
BASE COUNT          934 a          922 c          893 g         1158 t
ORIGIN      
        1 gtagaagctt tttcatgtcc tccatgtctt ggtcgagatc atctgggtgg atgtgtgcat
       61 accaaccccc agctttgatg tactcgtcgg gtgtatagcc ggtcattttt tcgaaagctc
      121 cggcaatcca aattgcttct cccaattcat tatcgttgat gcgactttca aacgcatagt
      181 ctgaacttac acttgagatc aagcgatact tttcttcact ttcctttgcg cgtttcaaag
      241 cattgtttac cgtccgcgag gataggtatt gcaaaactgc gctcatcggg aagatggcaa
      301 agctgaggat ccatgtcagg aacggcgggc gaatgacatc tttattgatc agaccattgc
      361 cttctgaaaa caccatcaaa agccctgaaa ccaaagacaa tgcggttaat cccagcgtaa
      421 accgattgcc gagcaaaatt cccgatatca tgatgacaag cgggtagcct aacaagtaag
      481 actcgccctg tacaccaatg ccggttgctg ctgaaacggt aaagaagatc caaaatgcga
      541 tgacttgtaa tagggaaaca gtacgaacat atcctcgttt caagagaata agaagaaata
      601 tattaatgaa ttccccaatg atgctttgcg ttaatgccct tgttgaagtg ccagataaaa
      661 atatctgagc atagatcaca taagggattg ggatcgcgat cagcccccaa agaataatat
      721 tgagcaaata cgcctgatgt gttttttgct catcttcaaa gacaggtggt ttaaaaaagt
      781 ctccgatgaa tttcatcccg ccttcacttt cttagctttc ttgcatctcc atctcgcatg
      841 gtcacaccgg tggcatccaa atgctctaac aaggcttgga catactttct agtcgtgttg
      901 aacatatcgc gaacctcagc taatgtgatc tgcccttgag attgaagtgt atttttgatt
      961 tttgctacca tttcatcata atccacttta cggaagatga catcttgaga aacgagcacc
     1021 aactcacctg tttcaatcaa cgcattgacg atttcttcat cggcttctgc ctggcattcc
     1081 ttgacgctgg gggtagcata agggttggat tcaaacttcc gcctcaatac ctggaccttg
     1141 acctgattct gcccatcgaa ttttatttca tgccccggct tcaataccgc tccgcgttca
     1201 tctaccaaat cgctttttga agcgaggaat gcaattgctg cgttaaagac cttgggtgtt
     1261 aactttagct tgctctttaa ttcctcacgc ggaatcccac gccgtagtgg gtattggctg
     1321 tgatatccgt tgactgtatc cagtatcgtt gtgcttagct tgctccaata ggggaatgcc
     1381 acggcaagcg cttcgggttt cagagaattt tccagtgaaa tcagctgacc attctgtaat
     1441 agttcattca aagcggactc tgcaacttta acttctagcc tagatttgct aaccatgtct
     1501 ttaatggacg caatttgcaa agccaatgcc gcctcaaaaa ggacatcagc gggagaacct
     1561 tgtgccagtg attcgagcga tttcaaaaca tctgaatcaa atcgtttgtg tctacctttg
     1621 ggttgatggt caactacggc gccaccgcca agtgtttcac cgggtgaggg tctgcgaagg
     1681 atatatcggt caccgcgcaa agcaacaatg gggtctcgta attcaagttg gagccaaccc
     1741 tcttcacccg ccttgagttc ttctgtgcct aataaacggg ctattgcgac ggtttcactt
     1801 gcccccacaa agaatttaac ttcatcgcca taagtaagtg gcttgctaat atcaggaagc
     1861 gccttaaagc gcacatcaat tcggcgcgtt gactggtatt gacctggatg ggcaagaaca
     1921 tctcctcggc gaatctgttc tacatctaca ccagaaacat tcacggcggt tcgcgaaccg
     1981 ggtacagcgc tttcttcttt tttcttatgc gtttgtaacc cccgaatacg tccttgcaac
     2041 ccactgggaa gaatttctac ttcatcgcca acagaaagat gcccatctct caatgtgccg
     2101 gtgacgactg tgccaaaccc gctcatcgtg aagatacggt caattggcaa acgtggacgg
     2161 ctcaggtctg ggcgggctgg tttttcttgt aatagggttt caagggcatg agtgagttct
     2221 tttaaacctg tccctgcacg agcagagacg cgcagaatag gagcttcctt gagaacggtt
     2281 ccacttacag cgtggcgaat gtctgcttca acgaggtcaa gccaacctga gtctgaggcg
     2341 aggtcggttt tggtcaagac gatcaaccca gcgggaatct gcaataagtc tagaattgca
     2401 aggtgttctt tcgtctgcgg catgatgcct tcatctgcag agatgacaag cagaaccgca
     2461 tcgatgccgc caatgccgga gagcatattt tcaatgaagt cacgatgacc gggtacatcc
     2521 acaatgccga gctcttcccc atttggcaga gtaagccagc caaagccaag ctcgatggtc
     2581 atttcgcggg ctttctcttc tttgaggcgg tctggatgcg tgcccgtcaa cgtttcgatg
     2641 agcgtggact ttccgtgatc aacgtgccct gctgtgccta tgactctcat aatttaattt
     2701 taaccacaat ttgatttaac gaaaataaaa aagtggaaat cttgcgacct ccactttgga
     2761 aagctctttt atttcttgac tggcttttta ccatgcccca taatgcgttg gtatgaagtc
     2821 atccattcgt gaacgacgtt catcatctct tgtaattgtt tttcagaagc atcggtggtt
     2881 tggtgtagtt gatcttgaag cacttgcgat acttcattga gagttgaaat actgccttcg
     2941 tatttgctca cttccttgcg aatgtcgcgg aaggactctt cggaagataa gctgtaccca
     3001 gtccaacgct tttggtcatc ggctttgaag ctgatccatt cctgacgcag gcgttcttcg
     3061 gcgagacgct gcatttcggt cacttcgttg atgcggcgtt ctaacttgga gttgagttcc
     3121 atgtacgtat cctgcgcttt tttggcattg cgcaaggttt catctaaacg ttgaatttgt
     3181 tgatccaaag tttcggcttc tttttggaag gaaacgaatt tgtcctgcca atctttccag
     3241 gcacgttcac ggtccacttg ggctacgcgt tgttgatcta agaacacagc ttgggcttgc
     3301 ttgcgttcca gttctgaaga aagtaattcg gtaatgcgcg tttcaatgtt gcgaatggta
     3361 tcgccttgaa tcgtggactt ttcgcgattt tcatctatac gtttgcgtaa tgcagtgagc
     3421 tcaccttgca ggtccgcgat gcgtttgagg tcatttttgc gggcttcatc tgccgctttg
     3481 atggataact tcacatcttc gttcgagcgc tttgtatctt ctacaactgt tttgaagtct
     3541 gcaatatttg ccaaggcgcg ttgaatttca ttaccgcgct ctttgagctg tttcttgatt
     3601 tctgtgagat cggtgctgac ctttagctga gtcaatgttt tgctgagctc gtttatgtca
     3661 gattgaaact gttttgtggt atcgcgctct gaacgagtgt gggctttgtc cacatcttcg
     3721 atcattttga ccagctcatt acgctgttta ccaaccatgg tctcaaattg atctacacgc
     3781 ttggcggcag gggtaatgtc tgtgacctgc ttgctcaggg ttttaatctg ctttgaaacg
     3841 gcatttactg tagtttcaaa ggaggcaagg ctttctttta acgaggtaag ggtttcctta
     3901 gtctcgc
//
LOCUS       DAOQJE010000234         3904 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_14304_length_3904_cov_6.332554, whole genome shotgun sequence.
ACCESSION   DAOQJE010000234 DAOQJE010000000
VERSION     DAOQJE010000234.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3904)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3904)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3904
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14304_length_3904_cov_6.332554"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1275)
                     /locus_tag="PLT08_07460"
     CDS             complement(<1..1275)
                     /locus_tag="PLT08_07460"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNE04339.1"
                     /translation="MIASRWKKVWSDFWSNRGRTFLTIATIAVGTFAVGFNSNLGLYM
                     NESMDGDFLSANPSEAEIYASGLNDDLVKKAREVPGVEAVEGYSSVQGQLIRPDGTKI
                     DISFTAAETPNALTLNFLKPAMGETSIPSYGKKEVILDASASSLGYTAGDTIVIEFDN
                     GKRRELTFTGYMHAVTGFPYNLAQQVNAYVTPDTLAWLGGNINQYGRLSISVSEKQTD
                     ADHVTAVAQAVADELKRAGSTVYFVSVYQPGHHFAYSITQGVFFVLGVLGYLTVLLSG
                     FLIVNTITALMTQHTRQIGIMKATGGSAWQIFFMYLVLIMSFGIGALLLAVPLADIAA
                     QTIGSGMASWLNFYPTDYKGYSITLIQQVIVAILVPLLAAILPIYNSVRITVREALSD
                     YGLGGNVKTKEETVGKNSVLVPRPIRISLRNTF"
     gene            complement(1272..2414)
                     /locus_tag="PLT08_07465"
     CDS             complement(1272..2414)
                     /locus_tag="PLT08_07465"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNE04340.1"
                     /translation="MAISFFKPKAEDSDTAERPIIDIRSVNKFYKTAVGDYHALSNVD
                     LQIQAGEFVSIIGKSGSGKTTLLNMITGIDRPSNGEVWVNGTGVHTLNENKMARWRGK
                     NLGVVFQFFQLLPMISVIENIMLPMDFCRMYSPAERRKRAMDLLELVELADHAHKLPT
                     ALSGGQQQRVAIARALANDPPVIIADEPTGNLDSKTAESVFVLFNDLVAKGKTIIIVT
                     HDSAMAKRTHRTALIADGEIVNEYVAKAMPTLSHTQLLQATKSTTPMTFEAGAMILSE
                     GTNADTFYIVSHGTVEVILPRPNQSDVVAVQLGPGKVFGEMEFFHEKKHRASIRASEA
                     GPVEVLAIGYDKLTELLSQSDVTREALREMAGKHNGENLSWRGVSS"
     gene            complement(2460..3581)
                     /locus_tag="PLT08_07470"
     CDS             complement(2460..3581)
                     /locus_tag="PLT08_07470"
                     /inference="COORDINATES: protein motif:HMM:NF027890.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HNE04341.1"
                     /translation="MKSPKLIITILLALALTACGNASAATPESTPIPVVEADDTIIAE
                     GKLEPITYTEISLNTSGLVSELPVEEGDRVEAGDVLAVVRSENAQSLEDAQAKATQEL
                     TEAYKEYRDAQSRLDDFDVPARWNGMTPTQAIASSLEKLDVARTEFEPYKHLSEKLIK
                     YEEPKPDFSEMTQEEMEFYEASLENHKNDDRPLNGEARAKKKALDEAWELYRISIRWL
                     ELESNYQNAKVRLANAQADFDALQDTDFSLDTAALRAILASAELRAPHSGVITDLNLK
                     VGEFATDAAPVATIADTSKWLVKTSDLTEIDVVNLEEGQPVTVKLDAIPGKEFKGNVL
                     SIGQNYSENQGDVVYEVTILLSEFDPSMRWGMTAVVTFE"
BASE COUNT          966 a          933 c          922 g         1083 t
ORIGIN      
        1 aaatgtattg cgcaaggaaa tgcggatcgg gcgtggaacc aacaccgaat ttttgcctac
       61 agtctcttct tttgtcttca cgttaccccc taaaccataa tcgctcaacg cttcacgaac
      121 tgtaattctg acgctgttat agattggcag gatggcggct aataacggaa ccaagattgc
      181 aacgataact tgttgaatca aagtaatgga atagccctta tagtctgttg ggtaaaagtt
      241 caaccaagat gccattccac taccgatggt ttgggcagca atatcggcaa gcggaacagc
      301 cagcaagagc gctccaatgc caaaactcat gatgagcacc aaatacatga aaaaaatctg
      361 ccaggcactt ccgccggttg ctttcatgat gccgatttga cgggtgtgtt gagtcatcaa
      421 tgcagtgatg gtgttgacga tcaaaaatcc gctaagcaat acggtgagat atcccaaaac
      481 gcccaacacg aagaacacac cttgtgtaat gctgtaggca aagtgatgcc ccggttgata
      541 cacacttaca aaatacaccg tagaccctgc tcgcttaagc tcatcagcaa ctgcctgagc
      601 tacagcagtg acatggtctg cgtctgtctg cttttcagaa acactgatcg agagtcttcc
      661 atattgattt atgtttccgc ctaaccacgc taatgtgtct ggcgtaacgt atgcatttac
      721 ttgttgcgct aagttgtagg ggaaacccgt cacggcatgc atatacccag taaaggttag
      781 ttcgcggcgt ttgccattat cgaactcgat aacaatcgta tctcccgctg tatatcccag
      841 agacgaagcg ctagcatcaa ggatgacttc ctttttgcca taactgggaa tactcgtttc
      901 tcccattgca ggtttgagaa aatttaatgt caacgcattg ggggtttctg ccgcagtgaa
      961 agagatatct attttcgttc catcagggcg aataagttgc ccctgtacac tagaataccc
     1021 ttccactgct tcaacaccgg gtacttcgcg cgccttcttg actaagtcat catttaagcc
     1081 tgaggcgtaa atctctgcct cagaagggtt ggcagacaga aaatccccat ccatactttc
     1141 attcatgtac aagccaagat tgctattgaa ccctacagca aacgtcccaa cagcaattgt
     1201 ggcaatggtt aagaaggtcc gcccgcggtt gctccaaaaa tcagaccaga cttttttcca
     1261 acgactggca atcatgatga gactccccgc caagacagat tctcgccatt gtgtttgcct
     1321 gccatctctc tcagcgcttc acgcgtaacg tcagactgac tcagcaattc tgtgagtttg
     1381 tcatatccga tagcaagaac ctctacaggt cccgcttccg aggcgcggat ggaagcacgg
     1441 tgcttcttct cgtggaaaaa ttccatttcg ccgaacactt ttccaggtcc gagttgaacc
     1501 gccacgacat ccgattgatt cgggcgcggc aaaataactt ccaccgttcc atgtgagacg
     1561 atgtagaacg tatctgcatt tgtcccttca gaaaggatca tggcacctgc ttcgaaggtc
     1621 atgggagtgg tagattttgt tgcctgtaaa agttgagtat gagaaagtgt gggcattgcc
     1681 tttgccacgt actcgttgac aatttcgccg tctgcgatga gtgcggtgcg atgggtgcgt
     1741 tttgccatgg cgctatcatg tgtgacgatg ataatggttt tgccttttgc gacaaggtca
     1801 ttgaatagca caaaaacaga ctcggctgtt tttgagtcaa ggttgcctgt tggttcatct
     1861 gcaataatca cgggcggatc attagcaagc gcacgcgcta tcgctacacg ttgctgttgt
     1921 ccacctgaaa gcgcggtggg gagtttatgt gcgtggtctg ccagttccac caattcaagc
     1981 aagtccatgg cgcgtttgcg gcgttcggca ggggagtaca tgcggcagaa atccatgggc
     2041 aacataatgt tttcgatcac agagatcatc ggcaaaagtt gaaagaactg gaatacaacg
     2101 cccagattct ttccacgcca acgtgccatt ttattttcat tgagcgtatg cacacctgtg
     2161 ccattgaccc atacttcgcc atttgatggg cgatcaatgc cggtgatcat gttcaataga
     2221 gttgtcttac cgctgcccga tttgccgata atgctgacga actcgcctgc ctgaatttgc
     2281 agatctacat tgctgagcgc gtgataatcg ccaacagcgg ttttgtagaa cttgttcaca
     2341 ctgcgaatat caatgatcgg gcgttctgcg gtgtcgctgt cttccgcttt cggtttgaag
     2401 aaggatatag ccattagtaa tttccttcaa cacgacatct atatgccgtg ttgactactt
     2461 tattcgaagg taacgacagc ggtcatgccc cagcgcatag acgggtcgaa ctcgctcaaa
     2521 agaatggtga cttcatagac cacgtcgccc tgattttcag agtaattctg accgatggag
     2581 agtacattgc ccttgaattc tttgccggga atggcatcca acttaacggt gacgggctgt
     2641 ccttcttcaa ggttgaccac atcgatctcg gtcaggtcac tggttttgac gagccatttg
     2701 gacgtatctg caatagttgc tacgggtgct gcatcagtcg caaattcacc aactttgaga
     2761 ttcaagtctg taatgacacc tgaatgaggt gcacgtaatt cagcgctcgc aagaatggcg
     2821 cgtagagctg cggtatcgag tgagaaatct gtatcttgca gagcatcaaa gtctgcttga
     2881 gcattggcaa gccgaacctt tgcattttga tagttgcttt ccaattccag ccagcgaata
     2941 gagatgcggt ataactccca agcttcatcg agtgcctttt tctttgcgcg cgcttctcca
     3001 ttcaagggac ggtcatcgtt tttatgattt tcaaggctgg cttcgtagaa ctccatctct
     3061 tcctgagtca tttcagagaa atcgggtttg ggttcttcat acttgattaa tttctctgaa
     3121 agatgcttgt acggctcaaa ttctgtacgg gcaacatcca acttttcgag agaggaagca
     3181 atcgcttggg taggtgtcat accattccag cgtgcaggga catcaaaatc atccaagcgg
     3241 ctttgggcgt cgcgatattc tttgtaggct tcggtcagtt cttgggtagc ttttgcttga
     3301 gcatcttcga gagattgcgc attttcagaa cgcacaacag caagcacatc acctgcttcc
     3361 acacgatctc cttcttccac gggtaattcg ctgaccagtc cgctcgtgtt tagggagatt
     3421 tctgtatagg taataggctc cagtttccct tcggcgataa tggtatcgtc tgcttcaacg
     3481 acgggaatgg gagtgctttc tggtgttgct gctgaggcat ttccgcaggc tgtgagagct
     3541 agtgcaagta agatcgtgat gataagttta ggcgatttca tgtccgcttc ctttgaagga
     3601 ttgattattt gttaagacag gatatataaa gaaaagtttc aaatttcagt gaacttttcg
     3661 tgtttactta cgacgccatg ggaagtttgg ttgcacagaa aaagagcaca atctaaaaga
     3721 attgtgctct ttccgtatta cttttctatt ctttttccca cccttgagaa ctgttttatt
     3781 tttccaacat catgctagct tcttcattga gccagcgccg accatcccga gcgagcaggt
     3841 cgtagccttc gggtggaccc cagctgccgg gtttgtaaac agccaacggc ggagtctgat
     3901 gcgt
//
LOCUS       DAOQJE010000235         3902 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_14316_length_3902_cov_6.391994, whole genome shotgun sequence.
ACCESSION   DAOQJE010000235 DAOQJE010000000
VERSION     DAOQJE010000235.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3902)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3902)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3902
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14316_length_3902_cov_6.391994"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(2..1189)
                     /locus_tag="PLT08_07475"
     CDS             complement(2..1189)
                     /locus_tag="PLT08_07475"
                     /inference="COORDINATES: protein motif:HMM:NF014176.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c3 family protein"
                     /protein_id="HNE04342.1"
                     /translation="MNNTNRLGCLTGTGLIAALVTLFLLVGVAFASGSKMFSSGALNA
                     QPGENMGGVTSHADIKECNTCHTAPWESATMADRCLDCHTDIATQMRDVAQLHGSIVQ
                     KSPAIACRDCHKEHRGSTASLTDLGENSFPHDTFGFSLNEHQRMENGDPITCENCHGN
                     DISTFASDSCQTCHSDMDIAFTQAHVLSYGNDCVACHDGVDRFDDFNHNAYAFKLEGE
                     HANVACTKCHLDARSIADLQSAPQDCYSCHRQDDVHNGENGTDCETCHNPSNWEDADF
                     DHSRTNFPLTGGHSNVECNQCHTNGTYKGLSSTCVTCHQDPAFHAGAFGTDCESCHSI
                     NAWSPAQFNLSHPEPRVDEGGSGIHHGGASCRECHPSSVFESSCDSCHSGGFEGGEGG
                     GDD"
     gene            complement(1199..1753)
                     /locus_tag="PLT08_07480"
     CDS             complement(1199..1753)
                     /locus_tag="PLT08_07480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE04343.1"
                     /translation="MLRGNIELWLAFLTCILIGAGYGVVTVWTREVPAASELFGHTLG
                     IIGFLLMLMTETFYSIRKRSRSAALGRMSTWLKFHIYMGLVGPFMVLLHSSWKFNGLA
                     GATTLLTAIIVFSGFIGRYIFTRIPRTLDGLEIEGTLSQEALKRARQMMALWHTVHIP
                     IGIALFLAAFVHMGGALYYATFLK"
     gene            complement(1799..3103)
                     /locus_tag="PLT08_07485"
     CDS             complement(1799..3103)
                     /locus_tag="PLT08_07485"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HNE04344.1"
                     /translation="MKYVVIGTGVAGISAVEAIRSVDRAGEIVMLGDDPHGYYSRPGL
                     AYILTGELQDESLFPKKREDYIPLKFKYKKARVIRIDRANKVLHLDDKTPLAYDKLLI
                     ATGASAIPLTVPGANLQGVMKLDHLEDARGILKHAKRGRTAIVVGGGITALELTEGLL
                     ARGVHVHYLLRGNRYWSSVLDEHESRIVEGRLQAEGVTLHFHSEVQEIIGKSGKVRGV
                     RLLDGRIMKCDMLAYAVGIKPRAELAKSADLALDRGILVNEYLQTNDPNIYAAGDVAQ
                     VYDPLTGRSVLDSLWSPAREQGYAAGLNMAGKSQAYVKMVPFNVTRLAGLTTTIIGAV
                     GHGRDEDIVGIARGDSETWRQLPDAIVAQGGFDINRVRLLVGEDYLIGAVVMGDQKLS
                     VPLQRMISEKINISSIRDTLLKKDTKVADVIVDFWSHHVAAV"
     gene            complement(3100..3366)
                     /locus_tag="PLT08_07490"
     CDS             complement(3100..3366)
                     /locus_tag="PLT08_07490"
                     /inference="COORDINATES: protein motif:HMM:NF024634.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HNE04345.1"
                     /translation="MSILNVFKTNASRHTTASSGYVVPDPYRCVQCGICSYNCPINID
                     VRAHAWRGEPVKHSECLTCGECVARCPRGVLRFERTDLFAEGAS"
     gene            complement(3602..3814)
                     /locus_tag="PLT08_07495"
     CDS             complement(3602..3814)
                     /locus_tag="PLT08_07495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HNE04346.1"
                     /translation="MSERIVGTVKWFNATKGYGFIGRDGGEDVFVHFSAIQTDGYRKL
                     EAEQKVEFSIEEGPKGLQAANVVPLQ"
BASE COUNT         1035 a         1014 c          915 g          938 t
ORIGIN      
        1 actaatcgtc cccaccgcct tcgccacctt caaagccacc gctatgacaa gagtcacagc
       61 tagactcgaa gacagatgag gggtgacatt cgcggcagga ggctccgccg tggtggatac
      121 cgctaccacc ttcgtcaacg cgcggctcag ggtgagacag gttgaattgg gcaggagacc
      181 aagcattgat cgagtggcaa gactcgcaat ctgtaccaaa ggcaccagca tgaaacgcag
      241 ggtcttgatg gcaggtgaca caggtagatg aaagcccttt atacgtccca ttggtatggc
      301 attgattaca ctccacgtta gagtgtccgc cagttaatgg gaagttggtg cggctatggt
      361 caaaatcggc atcttcccag tttgatgggt tatgacaggt ttcacagtct gtaccattct
      421 caccattgtg aacatcgtct tgtctgtggc aggagtagca atcttgcggt gcagattgca
      481 ggtctgctat agagcgtgca tcgagatgac attttgtaca agcgacgtta gcatgttcac
      541 cttctagctt gaaagcataa gcattgtgat tgaaatcatc gaagcgatct acgccatcat
      601 gacacgctac acagtcgttg ccgtaagaga gcacatgagc ttgggtgaag gcaatgtcca
      661 tgtcgctgtg gcaggtctgg caggagtcag aggcgaaggt cgaaatatcg ttgccatggc
      721 aattttcgca agtaatcggg tcgccgtttt ccatccgctg atgttcattt aatgagaatc
      781 caaatgtatc gtgaggaaaa gagttctcgc ccagatctgt cagcgatgcg gttgaaccgc
      841 gatgctcttt gtgacaatcg cgacatgcaa tggcgggcga tttctgtaca atgctgccgt
      901 gcagttgagc gacatcgcgc atttgggttg caatatcggt gtgacagtct aaacaacggt
      961 ctgccattgt ggcagattcc catggcgcgg tatgacaggt gttgcattct ttgatatcgg
     1021 catgcgatgt gacgccgccc atgttttcgc cgggttgtgc attgagtgcg ccggagctaa
     1081 acatttttga cccgctggca aacgcaacac ccacaagcaa aaatagagtg accagtgcgg
     1141 ctatcaagcc tgtgccggta agacaaccaa gtcgatttgt gttattcatg gctgtctcct
     1201 atttcaaaaa tgtggcgtaa tacaatgcac cgcccatatg aacaaaggct gcaaggaaca
     1261 atgcaattcc aattggaatg tgaaccgtat gccaaagagc catcatctgg cgggcacgct
     1321 tgagggcttc ttgtgaaagc gtaccttcta tttcaagccc atccagcgtg cgcggaatac
     1381 gagtgaagat ataacgcccg atgaatccgc taaagacaat gatggcggta agtaaggtgg
     1441 tagcacctgc caacccattg aacttccatg aagaatggag caacaccatg aacggaccga
     1501 ctagccccat atagatgtga aatttcaacc aagtggacat gcgtcccaat gccgcactgc
     1561 ggctgcgttt gcggatgcta taaaaagttt ctgtcatcaa catcagcaga aacccgataa
     1621 tgcccaaggt gtgcccaaaa agttcagaag ctgcaggcac ttcacgcgtc cagaccgtca
     1681 ctaccccata accagcccca atcaatatac aagtaagaaa agcgagccaa agttctatgt
     1741 ttccacgaag catttttgtt gccctcccga ggcaaaaatt tgaccgttaa caatgaagct
     1801 atacagccgc cacatgatgc gaccagaaat ccacgatcac atcggcaacc tttgtatctt
     1861 ttttcaacaa cgtatcacga atggaagaaa tatttatttt ttctgagatc attctttgca
     1921 aaggcacaga gagtttctgg tcgcccatca cgactgcgcc aattaggtaa tcttcgccaa
     1981 ccaacaagcg gacacggttg atatcaaaac cgccttgtgc cacaatcgca tctggcagtt
     2041 gacgccacgt ctcgctatcg ccacgggcaa tgcccacaat atcttcgtcg cgcccatgcc
     2101 ccactgcccc aataatggtc gtcgtcagcc cggcaaggcg cgtgacattg aacggcacca
     2161 tctttacata cgcctggctc ttgcctgcca tattcaaccc agcagcatac ccctgttcgc
     2221 gagcagggct ccacaaacta tccagcacag aacgcccagt caacgggtcg tagacttgag
     2281 ctacatcgcc cgccgcataa atatttgggt cattggtttg caaatattcg ttgactaaaa
     2341 ttccgcgatc caaagcaagg tctgcagact tggctagctc cgcccgaggc ttaataccta
     2401 cggcataggc aagcatgtca catttcatga tgcgcccatc taataaacgg actccgcgca
     2461 ctttcccaga tttgccaatg atctcttgca cttcagagtg aaaatgcaga gtcactcctt
     2521 cggcttgtaa acgcccctca acaatgcgcg actcgtgctc atcgagcaca ctgctccagt
     2581 aacgattgcc gcgcaacaga taatggacat gcactccgcg cgccagtaaa ccttcggtta
     2641 actcaagcgc tgtgataccg cctcccacca caatggcggt ccgcccacgt ttggcatgct
     2701 tcaaaattcc acgtgcatct tcaagatggt cgagcttcat cacgccttgc aagttcgcac
     2761 cgggaacagt caacgggatg gcagaggctc cggttgcaat gagcagctta tcgtacgcaa
     2821 gaggcgtttt gtcatccaaa tgcaaaacct tattcgcacg gtcaattcgt atgacgcgcg
     2881 cttttttata cttaaacttt agcgggatgt aatcttcacg tttcttcgga aaaagagatt
     2941 catcttgcaa ttcacctgtg aggatgtatg ccagccccgg gcgggaataa tacccatgcg
     3001 ggtcatctcc aagcatgacg atttcaccgg ctctatccac gctgcgaatg gcttcaactg
     3061 ccgaaatgcc tgctacgccg gtaccaatga cgacatattt catgaggcac cttcggcaaa
     3121 caagtctgtt cgttcaaagc gtaacacccc gcgcggacat ctcgccacgc actcaccaca
     3181 agtgagacat tcactatgct ttacaggctc tcccctccac gcatgggcgc ggacatcaat
     3241 attgatggga cagttatagc tacaaatacc gcattgcaca cagcgatacg ggtctgggac
     3301 gacatagcca gatgaggcag ttgtatgccg gctggcgttg gttttaaata cattaagaat
     3361 ggacaagaat atcctccatt tacagggtcg cttagaattt gaaaaagtta cggggcattt
     3421 ctatatctat actaaatctg cctgagggca tccaaatagt gtcgaatgtc ataaagatgt
     3481 acaaatgaaa tgagtttgaa aagaaatgaa acttctctta aaagtcttat ttcgtaaaat
     3541 ttaaaaaaaa cagggctgtc tcacgacagc cctgtctgtt aggcaaagat attttattat
     3601 cttactgaag gggcactaca ttggcagctt gcaagccttt gggaccttct tcgatggaga
     3661 actccacttt ctgttcggct tccaacttgc gatagccgtc tgtctgaatg gcgctgaagt
     3721 gaacgaacac atcctcgccg ccgtcgcgac cgatgaagcc atagcctttg gtcgcattga
     3781 accacttaac ggttccaaca atacgttctg acatctttct ttactcctgc tgtataaaaa
     3841 tttatgcccg tccacctaaa cgctttcaaa ggcgggtcgg gcacgcggag gcaagattat
     3901 ca
//
LOCUS       DAOQJE010000236         3894 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_14363_length_3894_cov_6.861422, whole genome shotgun sequence.
ACCESSION   DAOQJE010000236 DAOQJE010000000
VERSION     DAOQJE010000236.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3894)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3894)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3894
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14363_length_3894_cov_6.861422"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(127..1305)
                     /locus_tag="PLT08_07500"
     CDS             complement(127..1305)
                     /locus_tag="PLT08_07500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE04347.1"
                     /translation="MRCKTTRHSLIVSRLFLSLSLFATLLAACQPAAPTTDINAALTA
                     AFETAVAQINAPTATPQITDTPIPTATVPRTPPALPGTYQSGILKPNDIPRTYIVASC
                     QYLKDKWDSTKSAPGTVVMTIMSHRINQDAATSDPASLTISSKDLKRLMNSLHDAGFT
                     AINMQQMSDFMYNNAKIPQLSVLIIVDDRSSSSKYNDHFRKYYEDWGWPVVNAYIGLD
                     ERPDLWAENAVLSSEGWVDYEAHGYIHNIPISDGSTDDFITSEMGGVITTFQKYFNKT
                     PIAYIWPGGGFSKRAAEIGVQLGYKLGFTVNPRGPVMYNWIPQADAYNEDNPYAIPET
                     PAGNPLMTLPRYMDITAGDHIDEVRVMSQEAAAYAEQNKATELEYYNIVCAPTYGPIP
                     "
     gene            complement(1386..2474)
                     /locus_tag="PLT08_07505"
     CDS             complement(1386..2474)
                     /locus_tag="PLT08_07505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol-3-phosphate synthase"
                     /protein_id="HNE04348.1"
                     /translation="MANKKKVRVAIIGVGNCASSLVQGVQFYKNAKNDDVIPGIMHPQ
                     LGEYHIGDIEFTLAIDINATKVGKDLSEAIFAEPNNTYKFCDVPHLNVPVVRGMTHDG
                     LGKYLSQVIEKAPGSTADIVRLLKETKTEVVISFLPVGSEMATKWYVEQIIEAGCAFV
                     NGIPVFIASSEYWGKRFADAGLPILGDDIKSQVGATILHRQLATLFVDRGVKIERTYQ
                     LNFGGNTDFLNMLERERLESKKISKTNAVTSMIPYEINPDNVHVGPSDHVPWLTDRKW
                     AYIRMEGTTFGNVPLNMEVKLEVWDSPNSAGVMIDALRCAKIALDRELAGPIVGPSSY
                     FFKTPPKQFRDEIAKQKVEDFVSGKSDE"
     gene            2574..3839
                     /locus_tag="PLT08_07510"
     CDS             2574..3839
                     /locus_tag="PLT08_07510"
                     /inference="COORDINATES: protein motif:HMM:NF024583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNE04349.1"
                     /translation="MTDSQTKILSRLLEVTRSLDSEADLESSLRLILSAATELTASET
                     ASLLEYDETALNFFFKYVPWFNQDSQGGSTLRIPLNESIAGWVLLNKSSVTVNDAIND
                     PRHSKKVDEFAGITIHSVLGAPLIVRDVPIGVLEVFNKRSNYTEEDALIVENLAALAA
                     AAMEKDLLEKKVASSKEEARELDRMKNEFIAITSHELRTPLGLILGHSTFLKELLGGS
                     YEDQVDVIIRNASRLKEIIESLTSVDNYQSGGALVRSRKVSIARIIEDVIVSFSEMAN
                     KKKITIKKALQPGYELWVDVDAGKVAIVLSNIMKNAITFTNEGGEIIIRGEQHPDYVK
                     VSVKDNGIGIPTKDLPHVFDRFYQVEGHLTRRHGGMGLGLSVAKVMVEMHGGRIWADS
                     EEGKGSIFSFILPVRSEPPAPASSSPFSI"
BASE COUNT          961 a          810 c         1032 g         1091 t
ORIGIN      
        1 aacgtatttc ttggcgattg catataaata gaattgtaaa ccagtttttg agaatgagtt
       61 ttggcttatg taaacaggtc aggcattgcc tgacctgttt aggttgtact taaaaagaat
      121 tattaactag gggataggtc cgtatgttgg agcgcagacg atgttgtagt attccaattc
      181 ggtggctttg ttttgctcgg cgtatgcagc cgcttcttgg ctcatgacac gaacttcgtc
      241 gatatgatct cctgcagtga tgtccatgta gcgagggagg gtcattaagg gattcccagc
      301 tggcgtttca ggaatggcat atggattatc ttcattgtag gcatcggctt gaggaatcca
      361 gttatacata acgggtccgc gtgggttgac agtaaagccg agcttatacc caagttgaac
      421 accgatctca gcggctcgct ttgaaaaccc gccgccgggc cagatataag caatgggggt
      481 cttgttgaaa tatttttgga aggttgtaat cacacctccc atttctgagg tgatgaagtc
      541 atctgtagag ccatcactaa tggggatgtt atggatgtag ccgtgggctt catagtccac
      601 ccagccttca gaggaaagta cggcattttc tgcccaaagg tcggggcgtt cgtctaaccc
      661 gatatatgca ttgaccacgg gccagcccca atcttcataa tatttcctaa agtggtcatt
      721 gtattttgaa gaacttgaac gatcatccac aatgatgagc accgaaagtt gaggaatctt
      781 tgcgttgttg tacatgaaat ctgacatctg ttgcatattg atggcggtaa agccagcatc
      841 atgtaaggag ttcatcaaac gttttaagtc tttgctggaa atagtcaaac ttgcagggtc
      901 agaggtagcc gcatcctgat tgatgcggtg agacataatg gtcatgacta cggtccctgg
      961 cgcagatttg gtcgagtccc atttgtcttt taggtattgg catgaggcca cgatataggt
     1021 gcgagggatg tcatttggtt ttaggattcc ggattgatat gtgcctggca aagcaggtgg
     1081 cgtgcgtgga acggttgcag ttggaatggg agtgtcagta atttggggtg ttgcagttgg
     1141 tgcattgatc tgtgccacgg cggtttcaaa agcggctgtc agtgcggcgt tgatgtcggt
     1201 ggtgggggca gcgggttggc aggcggctag aagtgtggca aacaggctaa ggctaagaaa
     1261 gaggcgagaa acgattaacg agtgacgagt ggttttgcat cgcatgatga atttatatcc
     1321 ttttgtgatt gtcaataata acagaaaagg cgcaccagat ttctgtgcgc cttttctgtt
     1381 atttgttatt catcactttt gccagagacg aaatcttcca ctttttgttt tgcgatttca
     1441 tcgcggaatt gttttggcgg agtcttgaag aagtaagagg atggtccaac aatcggaccg
     1501 gcaagttcgc ggtcgagcgc gatcttggca cagcgtagag catcgatcat aacgccggca
     1561 gagttggggc tatcccacac ttcaagtttc acttccatgt tgagcggaac gttgccgaag
     1621 gtggtacctt ccatgcgaat gtatgcccat ttgcgatccg ttaaccaggg gacgtgatcg
     1681 ctgggaccga cgtgaacatt atctggattt atttcgtatg ggatcatgct ggtgacggcg
     1741 ttggtcttgg aaattttctt ggattcaagg cgttcgcgtt cgagcatgtt gaggaagtct
     1801 gtattgccgc caaagttgag ctgataggtg cgctcgatct tgacgcctcg gtctacgaac
     1861 agggtggcga gctggcggtg aagaatggtg gcaccgacct gacttttgat atcgtcaccg
     1921 agaattggca aaccggcatc ggcgaagcgt ttgccccaat attcagagga ggcaatgaat
     1981 accggaatac cgttaacaaa ggcgcaacct gcttcaataa tctgctcaac ataccatttg
     2041 gttgccattt ctgagcctac cgggaggaag ctgatgacaa cttcggtctt tgtttctttt
     2101 aataggcgta caatatcggc cgtggagccg ggtgccttct caatgacctg gctgagatac
     2161 ttgcccaacc catcatgggt catgccgcgc acgacgggta cattgaggtg aggcacgtca
     2221 cagaacttgt aggtattgtt cggctccgca aaaattgctt cagacaaatc tttgccaacc
     2281 ttggtggcat tgatgtcgat cgccagggtg aattcaatgt caccaatgtg atattcaccc
     2341 aattgcgggt gcataatgcc aggaatcaca tcgtcatttt tggcattttt atagaattga
     2401 acgccttgca cgagggaaga ggcacagttt cctacgccaa tgatcgccac acgtactttt
     2461 ttcttatttg ccatttgttt agctcctgag gtggattaca gtttccttat tgaattatga
     2521 gggtcgattt tcagatacaa ccgtgaagta taatcttacc caaggttatt tgtatgacgg
     2581 actcacagac gaaaatcctt tcacgtctcc tcgaagtgac acgcagcctc gactctgaag
     2641 cggatttgga gtccagttta cgtttaatcc tctctgccgc taccgaactg acggcgagcg
     2701 aaaccgcgtc tcttttggaa tatgatgaga cggctctaaa tttctttttc aaatatgtcc
     2761 cttggttcaa tcaagattca caaggtggaa gtacgctccg tattccgttg aatgagagta
     2821 ttgccggttg ggttttgctg aataaatcat cggtgactgt gaacgatgcg atcaatgacc
     2881 cgcgtcattc caaaaaggtg gatgaatttg caggcataac catccactcc gttttagggg
     2941 caccgttgat tgttcgcgat gtgccgattg gggttttgga agttttcaat aaacgcagca
     3001 actacacaga agaagatgct ctgattgttg aaaacttggc ggcactggcg gctgcagcga
     3061 tggaaaagga ccttcttgag aagaaagtcg cttcgtcgaa agaagaggcg cgtgaacttg
     3121 atcggatgaa gaatgagttt atcgcgatca catcacatga actgcgcaca cccttgggtc
     3181 tcatccttgg acattctacg ttcttgaaag aattattagg cgggagttat gaagaccagg
     3241 tggatgtcat cattcgtaat gcctctcgtt tgaaagaaat tatcgagagc ttaaccagcg
     3301 tggataacta ccagagcggt ggggcattgg tccgctcgcg caaagtttct attgcaagaa
     3361 ttattgaaga tgtgatcgtc tctttttctg aaatggcgaa taagaaaaag atcacgatca
     3421 aaaaagctct tcagcctggg tatgaattgt gggtggatgt agatgctggg aaagttgcca
     3481 ttgttctgag caatattatg aagaacgcta ttacatttac gaatgaaggc ggagaaatca
     3541 tcatccgtgg tgaacagcat ccggattatg taaaagtctc ggtgaaagat aacggaattg
     3601 ggattcctac caaagacttg ccacacgtgt ttgaccgctt ctatcaggtg gaaggtcacc
     3661 tcacacgccg acacggtggc atggggttgg gactttccgt tgccaaggtg atggttgaaa
     3721 tgcatggcgg gcgtatttgg gctgatagcg aagaaggcaa agggagtatc ttttccttta
     3781 ttcttcctgt tcgcagcgag ccgccagcgc cagcatcctc ttctcccttt tccatttgag
     3841 tcccgtcaca ccgttgacgg gatttttatt tccctataca ctcacgccca ttct
//
LOCUS       DAOQJE010000237         3887 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_14412_length_3887_cov_6.947808, whole genome shotgun sequence.
ACCESSION   DAOQJE010000237 DAOQJE010000000
VERSION     DAOQJE010000237.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3887)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3887)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3887
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14412_length_3887_cov_6.947808"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1395
                     /locus_tag="PLT08_07515"
     CDS             <1..1395
                     /locus_tag="PLT08_07515"
                     /inference="COORDINATES: protein motif:HMM:NF019982.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HNE04350.1"
                     /translation="YNTVENAADLRDIVTLLGYEKVNLYGASYGTRLALVAMRNHPEV
                     IRSAILDSVMPVETNWVTEFPNIANYTLTNLFNACKADPDCNKAYPDIETVFWELASK
                     LDEKPITLSTSAYPMGTVTETIDGSYLLSIIMGLSKTTRFIDTAPQTIYRVRDGDYST
                     LIAAQYSLPYGFEGINTGLYISVLCREHVLDTSPEKLLEAATQLRVKDFVWSPFYGHL
                     DKMFSACKTWGSTGPVLGEKDKVVSDIPSLVITGTFDGATPPQQGKQVADSLPNSYYF
                     EFPNQGHVPTASDDSGCAMQIALEFLDNPLTEPSRDCMSELKPVKFLVPYTGDPALEL
                     KRENLNGVSLIVPTEWKFVDDGFWWRGSASFDITQVTAFRANISVQDLVDYFSSSING
                     YRGFDGAPTPAGTREANGHAWTLYYATSNGRPVDVAAVDDKGNSIVIVMFSHPDEHDA
                     LYRTVFLPMVDSAK"
     gene            1500..2075
                     /gene="infC"
                     /locus_tag="PLT08_07520"
     CDS             1500..2075
                     /gene="infC"
                     /locus_tag="PLT08_07520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HNE04351.1"
                     /translation="MSANEFRVNEGIRVAEVRLIGPDGGNVGVVSIKEALKIAREAEL
                     DLVEVSPGANPPVCRVMDFGKFIYEKAKKEREAKKSQTKIEVKEIRLRPKTNEAHRGF
                     KVDDARRWLLQGHKVRVTVKFRGREMDYPEIALEDLKEIAADLADVAIIEVPPQMEGR
                     TMLVVMAPNKAGAPKKKEKPEQAETKNEAEA"
     gene            2158..2391
                     /locus_tag="PLT08_07525"
     CDS             2158..2391
                     /locus_tag="PLT08_07525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bL35 family ribosomal protein"
                     /protein_id="HNE04352.1"
                     /translation="MPRKAKPGKKFKLKTHKATSKRFRLTGAGVLVRTKGGKSHLRRR
                     TSDRTKALLSETIVVKGRKFVKRIKRLAPNMEG"
     gene            2489..2830
                     /gene="rplT"
                     /locus_tag="PLT08_07530"
     CDS             2489..2830
                     /gene="rplT"
                     /locus_tag="PLT08_07530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002507379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HNE04353.1"
                     /translation="MRVKGGPQGHLRHKKVLKITKGQRGSKSTLFKRANEAMLKSLWY
                     ATRDRRVRKRDLRKLWIARINAAARLNGTTYSKLVAAMKKANIELNRKMLADIAVRDP
                     QAFAAVVAQTK"
     gene            2894..3454
                     /locus_tag="PLT08_07535"
     CDS             2894..3454
                     /locus_tag="PLT08_07535"
                     /inference="COORDINATES: protein motif:HMM:NF013798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macro domain-containing protein"
                     /protein_id="HNE04354.1"
                     /translation="MNSILVEKKFALGQSLQIVQGDITIEEVDAIVNAANEQLAHGGG
                     VAWAISKKGGPKIQAESDAWVRKHGQVSHAHPAWTSGGALPAKYVIHAVGPVWINGRD
                     DENQKLQAAVTGSLQVADELKCESISMPAISTGIFGFPKDRAAGIIFETIGDYFDKHE
                     GSTVNVVRIVLFDQSTIDAFLKQWAE"
     gene            3451..>3887
                     /locus_tag="PLT08_07540"
     CDS             3451..>3887
                     /locus_tag="PLT08_07540"
                     /inference="COORDINATES: protein motif:HMM:NF019644.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase substrate-binding
                     domain-containing protein"
                     /protein_id="HNE04355.1"
                     /translation="MITSNQNSKIKLVRALLGRAKERREAGAFVAEGVRLVEEAVKAN
                     WTCRFALYDETLSERGKSQVESLRSRGVDVEEVSASVMKSISETEAPQGILAVLEQSQ
                     LPIPDSRNFILIPDQIRDPGNLGTLLRSAAASGVQAVLIPPETT"
BASE COUNT         1034 a          947 c         1019 g          887 t
ORIGIN      
        1 tataacacgg ttgaaaatgc agccgactta agagatatcg tcacattact gggatacgaa
       61 aaggttaact tatatggtgc ttcatatggg acgcgtctcg cattggttgc catgcgcaat
      121 catcccgagg ttattcgcag cgctatttta gattcggtca tgccggtaga aaccaattgg
      181 gtgacagaat ttcccaacat agccaattac actctaacga atttatttaa tgcctgcaaa
      241 gcagaccctg attgcaataa agcctaccca gatattgaaa ccgtattttg ggagcttgcc
      301 agtaaattag acgaaaaacc gataacttta tctacatctg cgtacccgat gggaacggtt
      361 acagagacca tcgatggctc ctatttatta tcgatcatca tggggttatc aaaaactacc
      421 cgtttcatag atactgctcc acaaaccatc tatcgcgtac gcgatgggga ttattcaact
      481 ttgatcgccg ctcaatattc attgccgtat gggtttgaag gcattaatac gggtctgtat
      541 atctcagttc tgtgtcgcga acatgttttg gatacatctc ccgaaaaatt gctggaagct
      601 gctactcagt taagagtcaa agattttgtt tggagccctt tttatgggca tttggataag
      661 atgttctctg cctgcaagac ttggggctca acgggtccgg tactcggcga aaaagataaa
      721 gtcgtcagcg atattcccag tttggtcatc accggaacct tcgatggtgc tactccgcca
      781 caacaaggca aacaggtggc agactcatta cccaatagct attattttga attccccaac
      841 caagggcatg tgcccaccgc aagcgacgac agcggctgtg ccatgcaaat tgctttggaa
      901 tttctggata acccgcttac agagcccagc cgagactgta tgagcgaatt gaaaccagtt
      961 aaattcttag tgccctacac cggcgaccct gcccttgaac tgaaaagaga aaacctcaac
     1021 ggagtctcgc ttattgtccc taccgaatgg aagtttgtag acgatggctt ctggtggcgc
     1081 ggatccgctt ccttcgatat cacacaggtc acagcatttc gtgcaaatat cagtgtgcaa
     1141 gacttggtgg actatttctc ttccagcatc aatggctacc gtggttttga cggtgctccc
     1201 accccagcag ggacgcgtga ggcaaacggt catgcctgga ccttatacta tgccacatcc
     1261 aatgggcgcc ctgtagatgt ggcagctgtt gacgataaag ggaattccat cgtgatcgtc
     1321 atgtttagtc acccagatga acacgatgcc ttgtatcgaa cggtcttcct accgatggta
     1381 gattcagcaa aataagccct ttttcgttga cgactcgcct gccttcatgg tattattcgc
     1441 ccgctaaaaa accagccatg ggctgggttt gttttgtcta acagcaagag gagaagcaca
     1501 taagcgccaa cgaatttcga gtaaatgaag gcatccgcgt cgcggaagtg cgcctgatcg
     1561 gtccagatgg tggcaacgtc ggagttgtgt ccatcaagga agcgttgaag atcgcacgcg
     1621 aagcggaatt ggaccttgta gaggtatcgc ccggtgcgaa cccacccgtc tgccgtgtga
     1681 tggactttgg taagttcatc tacgaaaagg cgaagaagga acgcgaagca aagaaatctc
     1741 agaccaagat cgaggtcaaa gagatccgct tgcgaccgaa gaccaacgaa gcccatcgcg
     1801 gcttcaaggt ggacgatgcc cgccgctggc tccttcaggg acacaaggtg cgtgtgaccg
     1861 tcaagttccg tggacgcgaa atggattatc ccgaaatcgc gctcgaagac ttgaaagaga
     1921 tcgccgccga tctggctgat gtcgctatca tcgaagtccc tccacaaatg gaaggacgca
     1981 ccatgttggt ggtgatggct ccgaacaagg ctggagcgcc gaagaagaaa gagaaacccg
     2041 aacaggctga aacaaagaac gaagcggaag cctgaggtga gcaagaccca aagatgttga
     2101 tcgtccgcgg gcagtgaaat gtccgcggtt tattttgtct gaaaggagta ctacgcgatg
     2161 ccacgcaaag ctaaacctgg caagaaattc aaactcaaga cccacaaggc gacatccaaa
     2221 cgctttcgcc tgaccggtgc tggcgtgctg gtgcgcacta aaggtggtaa aagccacttg
     2281 cgccgccgca cttcagaccg caccaaggct ctcttaagtg agaccattgt ggtgaagggc
     2341 cgcaagttcg tgaagcgcat caagcgcctc gcacccaaca tggaaggcta aatcttccgg
     2401 tcgtcgtaaa ttttttatgt gcggctttcg ggtgtgcgca actggtgtaa cccaccgacg
     2461 cccgcgagtt tgcaggaggt actaaacaat gcgcgttaaa ggaggcccgc aagggcactt
     2521 acgtcacaag aaagttttga agatcacgaa gggacagcgc ggctcgaaga gcacgctgtt
     2581 caagcgagcc aatgaggcca tgctcaagag cctctggtac gccactcgtg accgccgcgt
     2641 tcgcaaacgc gatcttcgca agttgtggat cgcccgtatc aacgctgcgg cccgcctgaa
     2701 cggcaccacc tacagcaagt tggtggcagc catgaagaag gcaaacattg agctcaatcg
     2761 caagatgctc gccgacattg ctgtgaggga cccacaagcc ttcgccgcag tcgtagcaca
     2821 aaccaagtaa ttttttatgt aggggcggat cgcgatccgc ccctacaatt tttaacgggg
     2881 gtaaaattga ttcatgaatt ccatcctagt agaaaagaaa tttgcattgg gtcagtcact
     2941 acaaatcgtg caaggtgaca tcaccataga agaagtggac gccattgtca atgcggcgaa
     3001 tgaacagctc gcccacggcg gcggagtggc ttgggcaatt tcaaagaagg gtggaccaaa
     3061 aattcaagca gaaagtgatg cctgggtccg caaacacgga caggtctcac acgcgcatcc
     3121 tgcctggacc tcgggcgggg cgctgcctgc gaagtatgtg atccacgctg tgggtccggt
     3181 gtggatcaat ggcagagatg acgaaaatca aaaattacaa gctgctgtga caggttcttt
     3241 gcaagtggca gatgaattga aatgtgagtc gatctctatg ccggcaattt ctacgggcat
     3301 tttcgggttt ccgaaagacc gagcagctgg aattattttc gaaacaatag gggattattt
     3361 tgataaacac gaaggttcaa ctgtaaatgt tgtgcgtatc gttctatttg accaatccac
     3421 aattgatgct tttctaaaac aatgggctga atgatcacat ccaaccagaa ttcaaaaata
     3481 aaacttgtgc gcgccttgtt ggggcgtgcc aaagagcgcc gtgaggctgg ggcatttgtc
     3541 gctgaagggg tgcggctggt ggaagaggca gtgaaagcga attggacatg tcgctttgcc
     3601 ttgtatgatg agaccttgag cgaaagaggc aagtctcagg tcgaaagttt aaggtcgcgc
     3661 ggcgtggacg tggaagaagt ctcagccagt gtgatgaagt ccattagcga gacggaagcg
     3721 ccgcagggta tccttgctgt gctcgaacaa tcccaactcc caattcccga ttcccgaaat
     3781 ttcatcctca tcccagacca gatccgcgac ccggggaatt tgggaacgtt gttacggtct
     3841 gcagcggctt cgggcgtgca agccgtttta attccgccag aaacgac
//
LOCUS       DAOQJE010000238         3881 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_14448_length_3881_cov_7.229482, whole genome shotgun sequence.
ACCESSION   DAOQJE010000238 DAOQJE010000000
VERSION     DAOQJE010000238.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3881)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3881)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3881
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14448_length_3881_cov_7.229482"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..950
                     /locus_tag="PLT08_07545"
     CDS             <1..950
                     /locus_tag="PLT08_07545"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HNE04356.1"
                     /translation="ISKRDLEILFDHIQAAIALVDRDGNLISWNSAFDAGKRQYPEVS
                     NLKDLFLQRDAESLRTKLAEGVANHWAGEFAITETDAAVLCDFWLVPTINEHMLFIAE
                     RIETEADAENMVEKLRKQVKLFQVESEVSKKLARNKQIEMEAVLAQAQEVAHVDPLTF
                     LPNRRTIIKDLQSEVMRAERYHSKFCISVVDIDKFKSINDTYGHPVGDEVLRHVALQL
                     RDGIRHPDVVGRYGGEEFIILLPNSDNNAAAEQASRLCRQIRSSVVRSKEHNITVTIS
                     IGIAQFHIGEDSWHSLLKRADNAMFEAKSRGRDRWVVGD"
     gene            complement(995..2218)
                     /locus_tag="PLT08_07550"
     CDS             complement(995..2218)
                     /locus_tag="PLT08_07550"
                     /inference="COORDINATES: protein motif:HMM:NF019040.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1501 domain-containing protein"
                     /protein_id="HNE04357.1"
                     /translation="MTTLLPRRQFLQGCSAAIAAMAGAKLTNLAFAKQDNPSDILVVI
                     FLRGGWDALNVVPPIDGDDRGFYEKARPDLKISSLLRLNDQFGLHPAMSPLYDLYQAG
                     KAGIIHSVGLNYDTRSHFDAQDYIELGTPGSKSTTSGWITRHLQATGISSILPVVSTT
                     GQPMSLLNFVPTVSVSTPDEFSLWDNGLRDSHMNALRQLYAGESLIHRAGSRTLDALD
                     IVRPLNGNTYQPANGAFYNDDELGNQLKTIAQMIKLDAGLRVAAVDFGGWDTHEYQNS
                     GNEGYMADLLNSLASGLANFYLDLDSGYTEKLSVVVISEFGRRLVQNDSYGTDHGHGS
                     VMLTLGGNVNGGQVFGSFPGLHNDQLYDHADLAITTDYRSVLGELLSKRMGNADLESV
                     FPGFSGNYNPLGIYR"
     gene            complement(2223..>3881)
                     /locus_tag="PLT08_07555"
     CDS             complement(2223..>3881)
                     /locus_tag="PLT08_07555"
                     /inference="COORDINATES: protein motif:HMM:NF020390.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1800 domain-containing protein"
                     /protein_id="HNE04358.1"
                     /translation="ACAPGEENAITQPFEGTTTLQNPVITPEAQTSLPPVSMAILALS
                     RLTFGIRPGDVDAFNALGATDDERLSAFVTQQLNPDSIDDSEFEARYANAGFETLHKS
                     HEQLYADHIASNPYDSNDDAYWQWYSKPTYELVDATILRAVYSKKQVVELLADFWHNH
                     FNVYFWQDDGVPLLVSYNRDVLRAHMLGNFRQMLEAVARHPSMLYYLNQNNSSDAGPN
                     ENFARELFELHTLGAENYLGVRDPNTVEKDANGIAVGYVDNDVYESARCLTGWRVDDD
                     LGDWEDGVEKTGNFLYYKPWHDRFNKLVLGKYIPADQEDMKDAHDVLDLLAYHPGTAR
                     HIARKLCTRFISDVPSESIVQAAANTFIQFKDAPDQLKRVMETILLSAEFKASWGGKV
                     KRPFEAAISMIRALNSETVRISGKLNWMLSLMGQAIFERRTPDGYPDTRDAWANSMSL
                     LYRWNLAVSLAENWMDDDDTGFKIASDVYGQTPAEIRSASSLADYWIQRILNRTLPDE
                     SRNAIIAVMAQEYGWDETLPEDHVHYVLPAMVEVILMSPDFQWR"
BASE COUNT          995 a          905 c         1007 g          974 t
ORIGIN      
        1 atatttccaa acgtgacctt gaaattctgt ttgatcacat tcaggcggcg attgccttgg
       61 tggatcgaga cgggaattta atttcgtgga attctgcatt tgacgcgggg aaaaggcaat
      121 acccagaggt ctcgaactta aaggaccttt ttttacagcg cgatgctgaa agcctgcgta
      181 cgaagttggc agaaggtgtt gcaaatcatt gggctgggga atttgccatc actgagaccg
      241 atgctgctgt gttgtgtgat ttctggctgg tgccgaccat aaatgagcat atgcttttca
      301 ttgccgagcg aatcgaaaca gaagccgatg cagagaacat ggtggaaaag ctccgcaagc
      361 aagtgaaatt gtttcaggtg gaaagcgaag tttcaaaaaa actagcacgc aataaacaga
      421 ttgaaatgga agcggtcttg gcgcaggcgc aagaagtagc gcatgtcgac ccactgacct
      481 tcctgccgaa tcgccgtacc atcatcaaag acttgcaaag tgaagtgatg cgtgctgaac
      541 gatatcattc taagttttgt atttcagttg tggatattga taaatttaag tccattaatg
      601 atacatatgg gcacccagta ggggatgaag tgttacgcca tgtggcactt caattgcgag
      661 atggcatccg tcaccccgat gtggtgggac gttatggcgg cgaagagttc atcattctgc
      721 tcccaaactc agataacaat gctgcggctg aacaagcctc acgtttgtgc agacagatac
      781 gcagttctgt ggtccgctcg aaagagcata acattacagt gaccataagc attgggatcg
      841 ctcaatttca catcggcgag gattcatggc atagcttgct caaacgggca gacaatgcca
      901 tgttcgaagc caaaagccgc ggacgtgacc gctgggtcgt tggagattaa ataaaaagag
      961 cctgtctcat tgagataggc tctttttcaa atagttatct gtaaatcccg agcgggttgt
     1021 aattgccaga aaagccgggg aagacagact caaggtcggc attgcccatt cgtttggaaa
     1081 gtaactctcc caatacggag cgataatctg tagtgatggc aaggtcagca tggtcgtata
     1141 gctggtcatt atgcaaaccg gggaaggaac cgaagacttg cccgccattg acgttaccgc
     1201 caagcgtaag catcacactg ccatgtccat gatcggtgcc gtaggagtcg ttttgaacaa
     1261 gccgacgccc aaattcacta atgacaacaa ccgataattt ctcggtgtat ccagagtcta
     1321 agtctaggta aaaatttgcc aacccagacg ccagactgtt gagcaaatct gccatgtagc
     1381 cttcattgcc gctgttctga tattcgtgcg tatcccaacc gccaaagtcc acggcagcaa
     1441 cgcgtaagcc ggcatcgagt ttgatcattt gtgcaatggt ctttagttga ttgcccagct
     1501 catcatcgtt atagaaggca ccattggcgg gttgatatgt atttccattc aacgggcgga
     1561 cgatatccaa ggcatccaat gtccgcgagc cggcacggtg aatgaggctt tccccggcgt
     1621 agagttggcg caacgcattc atgtgcgagt cgcgcaagcc gttatcccat aatgagaatt
     1681 catcaggcgt gctcacactg actgtgggaa caaaatttaa caacgacata ggctgccctg
     1741 tggtggaaac cactggcaag atcgaagaaa tgccggttgc ctgtaagtga cgtgtgatcc
     1801 accctgaggt agtgctcttg gaaccgggcg ttcccaattc aatatagtct tgagcgtcga
     1861 agtgactgcg cgtatcgtaa ttcaacccca cagagtgaat gatgcctgct ttaccggctt
     1921 gatacagatc gtataacgga ctcatggcag ggtgcagacc aaattggtca ttcaagcgca
     1981 atagactgga tatcttcaga tcggggcggg ctttttcgta aaagccacgg tcatctccat
     2041 caataggcgg aacaacattg agcgcatccc aaccgccgcg tagaaatatg acgacgagga
     2101 tatcgcttgg gttatcttgt ttagcaaaag cgaggttggt caactttgcg cccgccatgg
     2161 cggcaatggc ggctgaacat ccttgcagga actgacggcg tggaagtaaa gtggtcattg
     2221 gtctatctcc attgaaaatc ggggctcatt agaatcactt ccaccatggc aggcagaaca
     2281 tagtgcacgt gatcttcagg cagggtttcg tcccaaccgt attcttgtgc catcactgcg
     2341 atgatggcgt tgcggctttc atcgggtagc gtacggttca ggattctttg aatccaataa
     2401 tcggcgagag aagaggcgct gcgaatctcc gccggggttt gcccataaac atccgaagcg
     2461 attttgaagc cggtatcgtc atcatccatc cagttttctg ccaaacttac cgccagattc
     2521 cagcgataca agagcgacat ggagtttgcc catgcatcgc gggtgtcggg atagccatca
     2581 ggtgtgcggc gttcaaagat ggcttgaccc atcagactca acatccagtt gagtttgcct
     2641 gaaatgcgca ccgtttctga gttaagcgca cgtatcatgg atattgccgc ctcaaacgga
     2701 cgtttgacct ttccgcccca gctggcttta aactccgctg aaagcaggat ggtctccatt
     2761 acacgcttga gttggtctgg tgcatctttg aattgaataa aagtattggc tgcagcctga
     2821 acaatcgact cggatggcac atcggaaatg aagcgtgtgc aaagttttct tgcaatgtgg
     2881 cgagccgtgc ccgggtgata ggctaaaagg tcgagtacat catgagcgtc tttcatatct
     2941 tcctgatcgg caggaatgta tttgccgagc acgagtttgt tgaaacggtc atgccagggt
     3001 ttgtaataca ggaagttgcc tgtcttttca acaccatctt cccaatcacc gagatcatca
     3061 tccacccgcc agccggtgag acagcgcgca gactcgtaga cgtcgttatc aacatagccg
     3121 acggcaatgc cattcgcatc tttctcaacc gtgtttgggt cacgtacgcc cagataattc
     3181 tctgcgccga gtgtgtgaag ctcaaataat tcgcgtgcga aattctcgtt gggacctgcg
     3241 tcgctgctgt tgttttgatt caagtagtac aacatagaag ggtgccttgc gacggcttcc
     3301 aacatttggc ggaagttgcc aagcatatgg gcacgcaaca catcgcggtt gtatgaaacc
     3361 agcagtggca cgccatcatc ctgccaaaaa tagacattga aatggttatg ccagaaatct
     3421 gcgagaagtt ccacaacttg ttttttgctg tatacggctc gtaagatggt ggcatctact
     3481 aattcataag tgggtttgct ataccattgc cagtatgcat cgtcattaga gtcgtatgga
     3541 ttgctggcaa tgtggtctgc gtataactgt tcatgcgatt tatgcaaggt ctcaaacccg
     3601 gcattggcat aacgtgcttc aaactcactg tcatcgatgc tatctgggtt gagttgttga
     3661 gttacaaaag ctgaaagacg ttcgtcatct gttgcgccaa gggcattgaa agcatccacg
     3721 tcaccggggc gaatgccaaa tgtaaggcgg ctcaaagcta ggatcgccat cgaaacaggg
     3781 ggaagagacg tttgtgcttc gggtgtaata accgggtttt gtagagttgt tgtcccttca
     3841 aaaggctgcg tgatcgcgtt ttcttctccc ggcgcacaag c
//
LOCUS       DAOQJE010000239         3875 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_14482_length_3875_cov_5.263089, whole genome shotgun sequence.
ACCESSION   DAOQJE010000239 DAOQJE010000000
VERSION     DAOQJE010000239.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3875)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3875)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3875
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14482_length_3875_cov_5.263089"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..492)
                     /locus_tag="PLT08_07560"
     CDS             complement(<1..492)
                     /locus_tag="PLT08_07560"
                     /inference="COORDINATES: protein motif:HMM:NF015427.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PCRF domain-containing protein"
                     /protein_id="HNE04359.1"
                     /translation="MLDKLQAIEEKFEQLGQELLVVGTDYKRAAEISKERTSLEPLIE
                     KAKEYRQAVKSLEEAKEMLISESDAELLALAKADIADLEPKIEELEKVIKGLLVPKDP
                     RDDKNVIVEIRAGAGGDEAAIFAADLFRMYTRYTDEKKWKTEIMSENAIGVGGYKEVI
                     FEVK"
     gene            complement(606..755)
                     /locus_tag="PLT08_07565"
     CDS             complement(606..755)
                     /locus_tag="PLT08_07565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE04360.1"
                     /translation="MAGKNSKNQKNNKVMEEKVAKRQKTAQIMFAAFAILLIISMVLS
                     AVAKY"
     gene            complement(765..1145)
                     /locus_tag="PLT08_07570"
     CDS             complement(765..1145)
                     /locus_tag="PLT08_07570"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNE04361.1"
                     /translation="MKEVWIVDDDEEMARAVSLMLKLLDCTSTHYLGPRPAAQALLSG
                     KRPDMMILDINMPEVTGLDMLEFLRRRPEWKNLPVIMLSSEAADVMVDKALKLGADGY
                     VMKPVTIEELEKVMAQAFYKNIIK"
     gene            complement(1142..>1900)
                     /locus_tag="PLT08_07575"
     CDS             complement(1142..>1900)
                     /locus_tag="PLT08_07575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HNE04362.1"
                     /translation="AQWQADAHRQEQALSDLQTHLNLSAPPNRIECYDVSHTQGVATV
                     GAMVVFEQGVPAKRLYRKFNIESTSIGNPDDFASMEEMLTRRFRRWKGSQETESEVGS
                     KKDEAFSFLPDLIIIDGGKGQLGRVVKVMEQFEIFDKVPVVGLAKQEEEIFFPHKSDP
                     LLLPRHSQGLYLVQRIRDEAHRYGITAHRKKRSKLGLASQLDSIPEIGPTRRKALLKH
                     FGSMDKIKEASVEELMTVKGMTETAAKNVKAHLE"
     assembly_gap    1901..2000
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2001..3168)
                     /gene="uvrC"
                     /locus_tag="PLT08_07580"
     CDS             complement(<2001..3168)
                     /gene="uvrC"
                     /locus_tag="PLT08_07580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HNE04363.1"
                     /translation="MEVSEKLQGILATLPTKPGCYLYRNAEGTIIYVGKAINLKNRVR
                     SYFHADSSHDNKTRRLVRDIADIEWIVVGSELEALILEMNLIKKHRPKYNIRLKDDKR
                     YPYIKVHWNEPFPKITVTRQMEEDGSRYFGPYTSAWAVYQTLEVLRKIFPYLTCDREI
                     TGNDPRACLYYDIKLCTGPCIGAVSKDGYRQMISDLMEFLSGHSEPIVERVQADMLKA
                     SEEMRFEKAAALRDQLKAMQSIIERQKIVFGSDYADSDVIAMAREDGEACVQIFFIRG
                     GKLIGREYFILEGTEDTTDSQVMEEFVKQFYTEAANIPEQVMLPQELEERKIIAQWLR
                     SKRGGEKVELFVPNEGQPKDLVQMATENATETLSSLRAQWQADAHRQEQALSDLQ"
     gene            3306..>3875
                     /locus_tag="PLT08_07585"
     CDS             3306..>3875
                     /locus_tag="PLT08_07585"
                     /inference="COORDINATES: protein motif:HMM:NF016764.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0031267 - small GTPase binding [Evidence
                     IEA];
                     GO_process: GO:0006888 - endoplasmic reticulum to Golgi
                     vesicle-mediated transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Yip1 family protein"
                     /protein_id="HNE04364.1"
                     /translation="MEQMPSNTPVMESNPGPAGWVQTWIKAVTKPNEQTFIDISESPD
                     AKIQTALIWAAIAGLISGVVIGMGFALQQLIQGGNISDKVVTIAVAICGFPLAMAVIN
                     PIALSFSTALFQWVAKLFGGVGTFEKLIYPISAITVPMTIVSSVFSFLSSIPLVGACL
                     SIFSIAISIYATVLNIMAVKAVNRFDWGQA"
BASE COUNT          870 a          917 c          879 g         1109 t
ORIGIN      
        1 ctttacttcg aagatgactt ctttatatcc acctacgcca atggcgtttt cagacatgat
       61 ctccgtcttc cattttttct catctgtata gcgggtgtac atgcggaaga gatctgcggc
      121 gaagatggcg gcttcgtcac cgcctgcgcc tgcgcgaatt tccacgatga cgttcttatc
      181 atcacgtggg tctttcggca caagcaagcc cttgataact ttttcaagtt cttcgatctt
      241 gggctcgagg tctgctatat ctgctttggc gagagcgagc aattcggcgt cgctttcaga
      301 gataagcatt tccttggctt cttccaagct cttcactgcc tggcgatatt cctttgcttt
      361 ttcgatcaag ggttccaagc tggtgcgttc cttgctaatc tcggcggcac gtttgtagtc
      421 tgtgccgacg acaagcagtt cctgcccaag ctgttcaaat ttttcttcaa tggcttgtaa
      481 tttatctaac ataattcctc aagtcttgac gatatcgtct gcggtcggtt ttgtaaattt
      541 gcacaagaaa gcgcggcacc ataacgaaga ggcgccgcgc aaagaggtct tacgatgaaa
      601 ccaagctaat attttgcgac tgcagagagg accatggaga tgatcaaaag aatggcaaaa
      661 gcagcaaaca taatctgtgc cgttttttga cgcttagcga ccttttcttc catcacctta
      721 ttattttttt gattttttga attctttcca gccatttcaa ctactcactt tatgatattt
      781 ttgtagaatg cttgagccat taccttctca agttcctcga ttgtaacagg tttcatcaca
      841 tatccgtctg cgccgagctt gagggcttta tccaccatca catcggcggc ttcagaggag
      901 agcatgataa ccggtaaatt cttccattca gggcggcggc gtaggaactc tagcatgtcc
      961 aagccggtca cttcgggcat gttgatgtcc aagatcatca tgtctggtcg cttgcctgag
     1021 agcaatgcct gcgctgctgg gcgcggaccc agatagtgtg tggaggtaca gtccagcagt
     1081 tttagcatca aactgacggc gcgagccatc tcttcgtcgt catctacgat ccaaacttct
     1141 ttcattctaa atgagccttt acatttttcg cggcagtctc ggtcatgcct ttgactgtca
     1201 tcaattcttc gacactcgct tctttaatct tatccataga cccgaaatgt ttcaagagcg
     1261 ccttccttct agtaggacca atttccggga tggaatccag ttgagaagca agacccagtt
     1321 tcgagcgttt tttgcgatgc gcggtaattc cgtagcgatg ggcttcgtcg cgaatcctct
     1381 gcacgaggta gagtccctgt gagtggcggg ggaggagcag cgggtcactt ttgtgtggga
     1441 agaaaatctc ttcttcttgt ttggcgagac ccacaaccgg cactttatcg aagatctcaa
     1501 attgctccat tactttgacg actcttccca attgaccttt accaccatcg ataataatca
     1561 ggtctgggag gaaggaaaac gcctcatctt tcttggaccc gacttctgac tcggtttcct
     1621 gtgagccttt ccaacgccgg aagcgacggg tgagcatttc ttccatcgat gcaaagtcat
     1681 ccgggtttcc tatgctggtg ctttcaatgt tgaacttacg atacagccgt tttgccggaa
     1741 caccttgttc gaacacaacc atcgcgccga ccgttgctac gccttgggtg tgactcacat
     1801 cgtaacactc aatgcgattt ggcggggcag ataaattcaa atgagtttgc aaatcagaaa
     1861 gcgcctgctc ttgtcggtgc gcatcggctt gccattgcgc nnnnnnnnnn nnnnnnnnnn
     1921 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1981 nnnnnnnnnn nnnnnnnnnn tttgcaaatc agaaagcgcc tgctcttgtc ggtgcgcatc
     2041 ggcttgccat tgcgcccgca aagaggacaa ggtctcggtg gcattttcgg ttgccatctg
     2101 caccaaatct ttgggctgac cttcatttgg cacgaacaat tccacttttt cgccgccacg
     2161 ttttgaccgc aaccattgcg cgatgatctt tctttcttcc aattcttgcg gcaacatcac
     2221 ttgttcaggg atgtttgctg cttcggtata gaactgcttg acgaattctt ccatcacttg
     2281 actatcggtc gtatcctctg tcccttcaag gatgaagtac tcgcgcccga tcaatttgcc
     2341 gccgcggatg aagaatattt gcacgcaggc ttcgccatct tcgcgcgcca tcgcgatgac
     2401 atctgagtcc gcgtaatctg acccgaagac aattttttgc cgttcaatga tggattgcat
     2461 cgccttcaat tggtctcgta aggcagcggc tttttcaaaa cgcatctctt cagatgcttt
     2521 gagcatgtct gcttggacgc gctccacaat gggttcgctg tgtccgctga ggaattccat
     2581 caagtctgag atcatttggc ggtagccatc ttttgaaacc gccccaatgc acggacctgt
     2641 acacaactta atgtcgtaat acaaacatgc ccgtggatca ttgcctgtga tctcgcggtc
     2701 acaggtcaag taggggaata tttttcgcag cacttccagc gtctgataca ctgcccatgc
     2761 cgaggtatat ggaccaaaat aacgcgagcc atcttcttcc atttggcggg tgaccgtgat
     2821 ttttggaaaa ggttcattcc aatggacttt gatgtatgga tagcgtttat cgtctttgag
     2881 ccggatgttg tacttcggac gatgcttctt aatgaggttc atctcaagga tgagtgcctc
     2941 aagctctgag ccgaccacga tccattcaat gtctgcaata tcgcgcacca ggcggcgggt
     3001 cttgttatcg tgactagaat cggcgtggaa atagctccgt acgcggttct tcaaattgat
     3061 cgccttgccg acgtaaatga ttgtcccttc ggcgttgcga tatagataac agcccggttt
     3121 ggtgggcagg gtggcaagaa tgccttgtag cttctctgaa acttccattg cggcaattct
     3181 accgcttgtt atcgaaatta ggatatttgt tctgaaaatt gccgttgaaa tacagtacgg
     3241 tcaagcggaa tatcatcgct taaaatggat acgtcatcac tttggaaaga aaaaggagaa
     3301 ttcaaatgga acaaatgcct tcaaacacac ctgtcatgga atccaacccc ggacccgcag
     3361 gctgggttca aacatggatc aaagcagtta caaaaccaaa tgaacaaacg tttattgata
     3421 tctctgaaag cccagatgca aaaattcaaa cagcgcttat ttgggctgcc atcgcagggc
     3481 ttattagcgg ggttgtgata gggatgggtt ttgctctaca acaactgatt caaggtggca
     3541 atatttctga taaagtagtt actattgccg ttgcaatttg tggctttccg ctcgccatgg
     3601 cagttattaa cccaattgct ctatcttttt ccacagcatt gttccagtgg gttgcaaaac
     3661 tctttggcgg ggtaggcacg ttcgaaaaac ttatttatcc aatttcagcc attaccgtgc
     3721 cgatgacgat tgtttcaagc gtgtttagct ttttaagcag tattcctctt gtaggggcgt
     3781 gtttaagcat ttttagtatt gctataagta tttatgcgac tgtcttgaat attatggcgg
     3841 ttaaagcagt aaatcgtttt gactgggggc aggca
//
LOCUS       DAOQJE010000240         3871 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_14511_length_3871_cov_7.281971, whole genome shotgun sequence.
ACCESSION   DAOQJE010000240 DAOQJE010000000
VERSION     DAOQJE010000240.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3871)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3871)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3871
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14511_length_3871_cov_7.281971"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..202)
                     /locus_tag="PLT08_07590"
     CDS             complement(<1..202)
                     /locus_tag="PLT08_07590"
                     /inference="COORDINATES: protein motif:HMM:NF014591.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047429 - nucleoside triphosphate
                     diphosphatase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Maf family protein"
                     /protein_id="HNE04365.1"
                     /translation="MDKVKFVLASNSPRRRQLFGLGGWEFTVIVADVDETPFEHEPPR
                     DYVIRLAQAKASAVQPKAGLDAV"
     gene            366..935
                     /gene="xpt"
                     /locus_tag="PLT08_07595"
     CDS             366..935
                     /gene="xpt"
                     /locus_tag="PLT08_07595"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000310 - xanthine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0046110 - xanthine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HNE04366.1"
                     /translation="MNALQERILKEGKVLGGGILKVDSFVNHQVDPVLMDECGRAFAK
                     LFANVGATKILTAEISGIAPALTTGIHMGLPVVYARKTKPVTMPDQVYLTLAPSHTKG
                     RMVELIVSPEYLANNEKVLIIDDFLASGQTILGLVRLAEASGSKVVGIGALVEKSFEG
                     GRDALASVGVQIESIACIKSLDDGKIEFV"
     gene            969..1316
                     /locus_tag="PLT08_07600"
     CDS             969..1316
                     /locus_tag="PLT08_07600"
                     /inference="COORDINATES: protein motif:HMM:NF013401.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIT domain-containing protein"
                     /protein_id="HNE04367.1"
                     /translation="MIRKILHLRFIVFLIGWIFAHMSFAIPVKRLRETSNLIAFQHPS
                     PSYKVHLLITPKRQVQSLAELDPQDTAFFTDLYSTVQSLVKELDLKAYRLIVNGGEYQ
                     DFPHLHFHLISEM"
     gene            1350..2885
                     /gene="guaA"
                     /locus_tag="PLT08_07605"
     CDS             1350..2885
                     /gene="guaA"
                     /locus_tag="PLT08_07605"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HNE04368.1"
                     /translation="MNSIAILDFGSQYAQIIARRVRESQVYCELFPWDAPQEKILSIK
                     PKGFILSGGPKSVYEENAPFIQKFILESGLPILGICYGMQALTHALGGKVDPSAQREY
                     GHAEITPLVPGTMLDVLSKVWMSHGDKVTQLPEGFMALAKSGNSPFAAMGDMKRKYFG
                     VQFHPEVHHTPNGTELIKHFAIDICGVTPDWTPANIIEESVKRIREQVGNERVLAAVS
                     GGVDSSVAAALVHKAIGDQLVCVFVDTGLLRANEGAQVASAFREGLGAELITVDAADD
                     FLGALKGQTDPEMKRKIVGEKFIRTFEREAKNVGQPRFLVQGTIYPDVVESSGPDRSK
                     AAKIKSHHNVGGLPEDMQFELVEPLRYLFKDEVRLVGEALGLNSSLVWRQPFPGPGLT
                     VRCLGECTPERVARLRAADAILLEELSKAGFLGKGAQTSQAFVVLLPVRSVGVMGDMR
                     TYQEAAAIRAVATDDFMTADWARLPFELLAKVSSRIVNEVEGINRVVYDITSKPPATI
                     EWE"
     gene            2918..3820
                     /locus_tag="PLT08_07610"
     CDS             2918..3820
                     /locus_tag="PLT08_07610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE04369.1"
                     /translation="MNFDYGNILTRALKLTWKHKSFWLLLMIPVFISFMILAAIILPV
                     IFLTENEESMTLIMVLWAGIVIFGFFASFIIGAVSMNAVTLGILRAERGEGSTALMDV
                     VRDSFQYFKNAFGAVLIVQLSVGAVFTVFFLCMTALTLVTMGIASICLQPIMLLLTPL
                     SFLVGAVLYGALVAVIDEGLGALDAVKRAVQVVRDHVWKFVVISLIAYFGSMIVSSIF
                     TFPFMIPAMFGPALIESGVDITGNTFALIFIPFLCIFGVLMAFVSGVTGTFIAASMEI
                     SYLQLSRPVPEVIFAADAPKDATS"
BASE COUNT          916 a          972 c          918 g         1065 t
ORIGIN      
        1 taaccgcatc cagccccgcc ttgggttgaa ccgctgaagc tttggcttgt gccaatctga
       61 tcacataatc ccgtggaggc tcatgctcaa acggagtctc atccacatct gcaacgatga
      121 cggtgaactc ccacccgccc aagccaaata gttgtcgcct gcgcggagag ttagatgcta
      181 aaacgaactt tactttatcc atcaagagca agattctaac acagtcattt ttgatggatt
      241 aaaccgcgat aagtcgttgg aaatattggc aaagtactca gacaactttt tttgtcattt
      301 atcttctctc ttttcaaatc cggttgtaaa atcattcgat atctgtccag acaatcggag
      361 gcttcatgaa tgctttgcaa gaacgaattt taaaagaggg gaaagtgctc ggcggtggaa
      421 ttcttaaagt agatagtttt gttaatcacc aagtagaccc tgtgctcatg gatgagtgcg
      481 ggcgtgcgtt tgccaaactc tttgccaatg tgggtgcaac caaaattctc acagcagaaa
      541 tctcgggcat tgctccggcg ctcaccactg gcattcacat gggtttgccc gttgtgtatg
      601 ctcgcaagac caaacccgtt accatgcccg atcaggtgta tctcaccctc gccccttctc
      661 acaccaaagg tcgcatggtg gagttgatcg tctcaccgga atatcttgcc aacaacgaaa
      721 aagttctcat catcgatgac ttcctcgcca gcggacagac cattcttggg cttgtacgtt
      781 tggcagaagc ctctgggtcc aaagtcgttg gcattggcgc gttggtcgaa aagagtttcg
      841 aaggcgggcg tgatgctctc gcttctgttg gtgtgcaaat tgaatccatc gcttgtatca
      901 agtcactgga tgatggcaag attgagtttg tctaacacac acggttcact ctttgcaaag
      961 agtgaaccgt gattcgaaaa atcctacacc tgcgcttcat tgttttcctc atcggttgga
     1021 tcttcgctca catgagtttc gccatcccgg tcaaacgcct ccgtgagacc tcgaacctga
     1081 ttgcgttcca gcatccttct cctagttata aggttcattt gcttatcacc ccgaagcgac
     1141 aggttcaatc gttggcagag cttgacccac aagacaccgc tttctttaca gacttatact
     1201 ccactgtgca aagcctcgta aaagaacttg acctcaaagc gtatcggcta atcgtcaatg
     1261 gcggagagta tcaagatttt ccacatttac attttcatct tatttcagaa atgtaggggc
     1321 gagtcccccc tacgggatta ataaaacaca tgaattcaat cgccatttta gatttcggtt
     1381 ctcagtacgc ccaaattatt gcccgacgtg tgcgcgagtc acaagtctat tgtgaactct
     1441 ttccatggga tgcgccgcaa gaaaaaatcc tttcgataaa accgaaggga tttattttat
     1501 ctggcggacc caagtctgtt tatgaagaga atgcgccctt cattcaaaaa ttcatcctcg
     1561 aaagcgggct tcccatcctt ggcatttgtt atggcatgca agccctcacc cacgccctcg
     1621 gcggcaaagt ggacccatct gcccagcgcg agtatggtca tgccgaaatc acgccattgg
     1681 ttcccggcac catgttggat gtgctctcca aagtttggat gtcacacggc gataaagtga
     1741 cacaactccc cgaaggtttc atggcactcg ccaaaagtgg aaacagtccc ttcgcggcga
     1801 tgggcgatat gaaacgcaaa tattttggtg tgcaatttca ccccgaagtg catcacaccc
     1861 cgaacggcac cgaactcatc aagcacttcg ccatcgacat ttgtggcgta acgcccgatt
     1921 ggacccccgc taacatcatc gaagaaagcg taaaacgaat tcgtgaacaa gtgggcaacg
     1981 aacgtgtgct cgcagccgtt tccggtggcg tggattcctc tgttgcggcg gcattggttc
     2041 ataaagcgat tggcgatcaa ctggtctgtg tcttcgtgga tacgggactc ttgcgcgcca
     2101 atgaaggcgc acaagtcgct tcggcatttc gggaaggctt gggtgctgaa ctcatcaccg
     2161 tcgacgccgc tgacgatttt ctcggtgcat tgaaggggca aactgaccca gagatgaagc
     2221 gcaaaattgt cggcgagaaa tttatccgca cctttgaacg cgaagccaag aatgtgggtc
     2281 aacctagatt cttagtgcaa ggcacgatct accccgatgt agttgagtca tccggtcccg
     2341 accgcagcaa agcagccaag atcaagtctc atcacaatgt gggcggactc cctgaagata
     2401 tgcaatttga actcgtggaa ccgttgcgct atttattcaa ggacgaagtc cgtttggtag
     2461 gtgaagccct cggcttgaat tccagtttgg tgtggcgtca accgttcccc ggtcccggtc
     2521 tgaccgtgcg ctgcctcggg gagtgtacgc ccgagcgtgt ggcacgtctg cgggcggcag
     2581 atgcgatcct tctggaggaa ttatccaaag caggttttct gggcaagggc gctcaaacgt
     2641 cacaggcatt tgttgtgtta ctcccggttc gttcggtggg cgtgatgggt gacatgcgaa
     2701 cctatcaaga agcagcagcg atccgcgctg tagcaacgga tgacttcatg accgctgatt
     2761 gggctcgctt accatttgaa ttattagcaa aggtctcgag ccgtattgtc aatgaagtag
     2821 aaggcattaa tcgtgtagtc tatgatataa cgagcaaacc gcctgcaacc attgagtggg
     2881 agtaaatcaa ttttcgattg acaattggga gatggaaatg aacttcgact atggcaatat
     2941 cttaacccgc gcattaaagc tcacctggaa gcataaatcg ttttggcttc tcttgatgat
     3001 tcccgtgttc atttctttca tgattcttgc agcaatcatt ttgccggtca tctttttaac
     3061 tgaaaacgaa gaatctatga ctctgatcat ggtgctttgg gctgggattg tcatttttgg
     3121 tttttttgct agttttatta tcggcgcagt tagtatgaat gcagtcacac ttggcatttt
     3181 gcgagccgag cgcggtgaag gttctactgc acttatggat gtagtccgcg atagttttca
     3241 atatttcaag aatgcttttg gtgcagttct tatcgtccaa ctcagtgtgg gtgccgtatt
     3301 tacagttttc tttctctgta tgacagctct cacccttgtc accatgggta ttgcatccat
     3361 ctgcctacaa ccgatcatgc ttttgctcac tcccctatca tttttagtgg gtgccgtcct
     3421 ctatggagcg ttggtagcgg tcattgatga agggctgggc gcattggatg cagtcaaacg
     3481 tgccgtgcaa gttgtccgtg atcatgtgtg gaagtttgtc gtcatttcgt tgatcgccta
     3541 tttcggatcc atgattgtct ccagcatttt cacctttccc ttcatgattc ctgccatgtt
     3601 cggacccgct ctcattgaat ctggagtaga catcaccgga aacacctttg ctctcatctt
     3661 tattccattt ctttgcatat ttggagtact gatggcgttc gtctctggtg taacggggac
     3721 ttttatagcc gcctccatgg aaatttcgta cctacaactc tctcgccctg tcccagaagt
     3781 catctttgcc gccgatgccc caaaagatgc gacttcataa tcaccctcca cgtacataag
     3841 gatagaacgg cgcagaccgt tcactgtcac c
//
LOCUS       DAOQJE010000241         3865 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_14551_length_3865_cov_5.889764, whole genome shotgun sequence.
ACCESSION   DAOQJE010000241 DAOQJE010000000
VERSION     DAOQJE010000241.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3865)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3865)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3865
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14551_length_3865_cov_5.889764"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..698
                     /locus_tag="PLT08_07615"
     CDS             <1..698
                     /locus_tag="PLT08_07615"
                     /inference="COORDINATES: protein motif:HMM:NF020893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HNE04370.1"
                     /translation="GFLNLGLGTFYFEIIHLLGKSSPHRSMVLNFDFIWRTIPTMHLE
                     QLITHFGYPALLLGLLLEGETVLVLAAFMAHRGYLDLPLVILLGGLVAFASDQFFFWM
                     GRIQGNTFLANRPAWQPHVEKAKALLDRNSTLLFLSVRFMYGLRTVLPFVLGMSKLNT
                     KKFVLLDFIGAFLWAITFGLAGHFIGHVMTLIFDDVKEHELPVMIGIALIGIGIWLYR
                     RNNNKDKDGKDHE"
     gene            691..1245
                     /locus_tag="PLT08_07620"
     CDS             691..1245
                     /locus_tag="PLT08_07620"
                     /inference="COORDINATES: protein motif:HMM:NF017970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1003 domain-containing protein"
                     /protein_id="HNE04371.1"
                     /translation="MNNHSQEQFKNLFLEKMETFTRNEWELLRHIMDRRHVTRNVNKE
                     FDDQLTFGQRLADNVAKFGGSWTFIIIFGLILLTWVFLNSVVLARLNDAFDPYPYILL
                     NLFLSMLASIQAPIIMMSQNRQATKDRMDAAHDYEVNLKAEMEIETLHQKLDELRDKQ
                     WAELVEMQQEQIRLLTKLLEEKSK"
     gene            1498..2058
                     /locus_tag="PLT08_07625"
     CDS             1498..2058
                     /locus_tag="PLT08_07625"
                     /inference="COORDINATES: protein motif:HMM:NF013936.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HNE04372.1"
                     /translation="MRIAKHFRKDQETLQRFLDVFGAGSAMLGGGNKNVQAGFFIHGS
                     TFIHEYVDGNFFRKEELLMKTLEDAGFPVNSGVIGNMKDEHAKCRQAADLLSKAASDW
                     QGGDAGARIEVGWAASEYTSALRQNLDRLKNLVFPLLDQNIPPEEESAIIDGLNQIAL
                     ENSKEIEAGKYTKLLETLEEELSDWK"
     gene            2196..2651
                     /locus_tag="PLT08_07630"
     CDS             2196..2651
                     /locus_tag="PLT08_07630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HNE04373.1"
                     /translation="MPTHFEGSRAERRTLETFIKLTRCTNSVLGELSARQTTGDLTPS
                     QFAVLEALYHIGSMTQGEVSSKVLKSTSNLTTVIDNLERDGYVRRERDAEDRRVIHVH
                     LTEAGTSKIEAVLPNHVGALVEIFSVLSASEQETLGELCKKLGKGRGSA"
     gene            2615..2986
                     /locus_tag="PLT08_07635"
     CDS             2615..2986
                     /locus_tag="PLT08_07635"
                     /inference="COORDINATES: protein motif:HMM:NF036395.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5519 family protein"
                     /protein_id="HNE04374.1"
                     /translation="MQETWQGQGVGMSVKGAQARITQSVTSWAGVTVQPHRFGGVEYV
                     IGRREVGHIHGDHMVDIPFPKKVRDVLVAEGRAQPHHLLPETGWISFYLQQDDDVEKA
                     IALFRESYEIAQKQKTKKLEV"
     gene            2988..>3865
                     /locus_tag="PLT08_07640"
     CDS             2988..>3865
                     /locus_tag="PLT08_07640"
                     /inference="COORDINATES: protein motif:HMM:NF017206.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NmrA/HSCARG family protein"
                     /protein_id="HNE04375.1"
                     /translation="MNNKIIAVTGATGQQGGVVARKLLADGWQVRALTRDVNKPAAQE
                     LKALGAELFAGDLDNRAELDAAFKGVYGVFSVQNFWLPNVGFDGEIKQGKAVADAAKA
                     AGVQHLVYSSVGGADRAPGVQHFESKWEIEKYIQSLNLPFTILRPVAFMDNYNWSRAY
                     ILSGTFTGFGLRADKTLQIIAAEDIGVFAALAFTNPKEYLGKTLEIAGDELTEPQIAE
                     TLAKVIGRPVNIAQKEENDQEMNAMLRFFNGKGYTADIAALRKLHSGLLSFEQYLRKN
                     GWENQQPIPIPENTGWG"
BASE COUNT         1011 a          865 c          987 g         1002 t
ORIGIN      
        1 tcggtttcct taatcttggt ctaggcacgt tctactttga gattatacat ttactaggaa
       61 aatcctcccc ccaccgtagt atggttttaa attttgattt tatatggcgt acaattccga
      121 ccatgcatct tgaacaactg attactcatt ttggttatcc cgccctgttg ttgggactct
      181 tgctggaagg tgaaacagtc ctagtccttg cggctttcat ggcgcatcgc ggatatttag
      241 acttgccgtt ggttattttg ctcggtggct tagttgcctt cgcttccgac caatttttct
      301 tttggatggg gcgtattcaa ggaaatacgt ttttggcaaa tcgacctgcc tggcagccgc
      361 atgtagaaaa agccaaagct ctacttgacc ggaactccac tctgcttttt ctcagcgtgc
      421 gttttatgta tggcttaagg accgtattac cttttgtgct tggtatgtcc aaactgaata
      481 caaagaaatt tgttttatta gattttatag gtgctttttt gtgggcaatt acttttgggt
      541 tggctgggca ttttattggg catgtgatga cattgatctt tgatgatgtg aaagagcatg
      601 aactgcccgt gatgatcggg atcgctttga taggaattgg catttggttg tatcgccgta
      661 acaataataa agataaggat ggtaaagacc atgaataatc attctcagga acaattcaaa
      721 aacctgtttt tagaaaaaat ggaaacattt accagaaatg aatgggagct gcttcgccac
      781 atcatggatc gcagacacgt gacccgtaac gtcaacaagg agtttgatga ccaactaacc
      841 tttggtcagc gcctcgcgga taatgttgcc aaatttggcg gatcatggac gttcatcatt
      901 atctttggct tgatcttatt gacctgggtt tttctcaact cggttgtgct tgctaggttg
      961 aatgatgctt ttgacccata cccttatatt ttgctaaatc tctttctttc catgttggcg
     1021 tcgattcagg ctcctattat tatgatgtct caaaaccgcc aggcaacgaa agaccggatg
     1081 gatgcagcac atgattacga agtgaactta aaagcagaga tggagatcga aacattgcac
     1141 cagaaattag atgaactgcg cgataagcaa tgggcagaac tggtggaaat gcaacaggaa
     1201 cagattcgat tgttgacaaa actgttggaa gaaaaaagca aataaacgtt tgtataaaac
     1261 aggtcggagc aactctgacc tgttttgttt ccaatcggga acactcgtga tagcccctta
     1321 ctaatgccag cggactggcg ctctgtcctt ttggggttgt ttgcgtcttg gtttggaatg
     1381 agatcagttg gctaaattag catacaattg cacgggttca ttgctgtttg tttctattaa
     1441 agggcggtaa aattttaaag caaagaaaaa gtcccgtttt actgacagga aacccatatg
     1501 cgtattgcga aacattttcg gaaagatcaa gaaactctcc agcggttttt ggatgtattt
     1561 ggcgcagggt ctgccatgct tggcggaggt aataaaaatg ttcaagcggg ttttttcatc
     1621 catggcagca ccttcatcca tgagtatgta gacggaaatt tctttcgcaa ggaagaactg
     1681 cttatgaaaa ctctcgaaga tgccgggttt cccgtcaact cgggcgtgat cggaaatatg
     1741 aaagatgaac atgcgaaatg caggcaagcc gcagacctgc tttccaaagc cgccagtgac
     1801 tggcagggcg gggatgctgg cgcccgaatt gaagtgggct gggcggcgag cgaatacaca
     1861 tctgccttgc gccagaattt ggaccgcctc aaaaatctcg ttttcccgct gcttgatcag
     1921 aacatcccgc cggaagagga atctgcgatt attgatgggt tgaatcaaat tgcactcgag
     1981 aatagcaagg agatcgaggc tgggaaatac actaaactgc tcgaaacgct tgaggaagaa
     2041 ctcagcgact ggaagtaatc atccctgacc catggacccg cctaaggcgg gtttttgatt
     2101 cctacccaaa cggctagttt tatgcctctt gactctgagg gtaagttttg ataatatttc
     2161 gatatcaaac tatttgatgt cgaaagagat ttgatatgcc cactcatttt gaaggctccc
     2221 gcgccgagag gcgcaccctg gaaacgttca tcaagctcac ccgctgtacc aactcggtgc
     2281 ttggggaact atctgcccgc caaaccaccg gcgacctgac cccttcgcag tttgctgtgc
     2341 tggaggcgct ctatcacatc ggttcgatga cgcaaggcga ggtcagtagc aaggtgctga
     2401 agagcacgag caacctgacc actgttatcg acaacctcga acgtgatgga tacgtccgcc
     2461 gcgagaggga tgcagaagac cgcagagtga tccatgttca tttgacagaa gcaggcacga
     2521 gcaagatcga ggcggtgctt cccaatcatg ttggggcgct ggttgagata ttcagcgtgt
     2581 tgagtgcatc cgaacaggag acgcttggcg agttatgcaa gaaacttggc aagggcaggg
     2641 ggtcggcatg agtgtgaagg gagcgcaagc acgcattact caatctgtca catcatgggc
     2701 aggtgtgacc gtacagccgc atcgctttgg cggtgtggaa tatgtgatcg ggcggcgtga
     2761 agttgggcac atccacggcg accatatggt ggatattccc ttcccgaaga aagtgcgcga
     2821 tgtgttggtc gcagaaggac gcgcacagcc gcatcatctt ctgcctgaaa ccggttggat
     2881 cagtttctac ctgcagcagg atgatgatgt ggaaaaggca attgcattgt ttcgtgaaag
     2941 ttatgagatc gcacaaaaac aaaaaactaa aaaattagag gtatagaatg aacaataaaa
     3001 ttattgcagt gaccggtgcc actggacaac aaggcggtgt cgttgcccgc aaacttttag
     3061 cagacggctg gcaggtacgc gcactgaccc gcgatgtgaa caaacccgcc gcacaagaac
     3121 tcaaagctct cggcgcagaa ctcttcgcag gcgatctgga taaccgagct gaacttgatg
     3181 ccgcgttcaa gggtgtgtat ggcgttttca gtgtgcagaa cttctggctg cccaatgtag
     3241 gtttcgacgg cgagattaag caaggtaaag ccgttgccga tgccgctaaa gccgcaggcg
     3301 ttcaacactt ggtgtattcc tccgtcggcg gtgcagaccg cgccccgggt gtgcagcact
     3361 tcgaaagcaa atgggagatc gagaagtaca ttcaatcttt gaatcttcca tttaccatcc
     3421 tgcgcccagt cgcattcatg gataactaca actggagccg cgcatatatt ttgagtggta
     3481 cgtttactgg ctttggtttg cgcgctgata aaactttaca gatcattgcc gcagaagaca
     3541 ttggtgtttt tgctgccctc gcttttacca acccgaagga atatcttggc aagacgcttg
     3601 agatcgctgg tgacgaattg accgaaccgc agattgccga aacacttgca aaggtcatcg
     3661 gacgccccgt caacattgcg caaaaggaag aaaacgacca agagatgaac gccatgctgc
     3721 gtttcttcaa cggcaagggc tacaccgcgg atattgctgc gcttcgcaaa ctccactcgg
     3781 gcttgctcag cttcgaacaa tatcttcgca agaacggctg ggaaaatcaa cagcccatcc
     3841 caatcccaga aaacacgggt tgggg
//
LOCUS       DAOQJE010000242         3849 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_14659_length_3849_cov_5.443068, whole genome shotgun sequence.
ACCESSION   DAOQJE010000242 DAOQJE010000000
VERSION     DAOQJE010000242.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3849)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3849)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3849
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14659_length_3849_cov_5.443068"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..975
                     /gene="trpB"
                     /locus_tag="PLT08_07645"
     CDS             <1..975
                     /gene="trpB"
                     /locus_tag="PLT08_07645"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HNE04376.1"
                     /translation="QIYLKREDLAHTGAHKINNALGQALLVKRMGKKRIVAETGAGQH
                     GVASATAAALLGLECVVYMGSVDIARQEPNVFRMKLLGAEVRPVESGTKTLKDAINEA
                     IRDWVTNVRDTHYLLGSALGPHPYPTIVREFQSVIGREAREQMLMDAGRLPDTVIACV
                     GGGSNAIGVFSGFVNDEHVELIGVEAGGSGIASGKHAARFGDPEKARVGVIHGTRTYV
                     LQDEDGQIAETHSVSAGLDYAAVGPEHAMMRDNERAFYTSATDEEALNAFQMLCHTEG
                     IIPALESSHAVAEVIKKAPTMRKDQVILVNLSGRGDKDLNTVIKELGN"
     gene            1045..1812
                     /gene="trpA"
                     /locus_tag="PLT08_07650"
     CDS             1045..1812
                     /gene="trpA"
                     /locus_tag="PLT08_07650"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: protein motif:HMM:TIGR00262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HNE04377.1"
                     /translation="MNRLESAFANKPIFMPYFPLGYPDVETSVDVIEALAKNGADLIE
                     VGLSFSDPLADGPVIQQATQVALEKGITVKKLLAAVKELRARGVTIPLILMGYYNPML
                     AYGLEKFIHDAREAGADGFIIPDLPLEEAEEFQSINGDMPLIQMLAPTSPDERMEAIA
                     RNAKGFIYLVSVTGVTGERREISNDLGALIARVRAHTSAPVCVGFGIGTPEQAQQVGQ
                     LADGVIVGSACVKTIGKSMNPVETARQFSREFKDALK"
     gene            complement(1897..2256)
                     /locus_tag="PLT08_07655"
     CDS             complement(1897..2256)
                     /locus_tag="PLT08_07655"
                     /inference="COORDINATES: protein motif:HMM:NF013098.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HNE04378.1"
                     /translation="MLNLNSVMISTMQPAVLAAFYEKVFGKPADMADQENGFWGWQVG
                     SGFVGILTHSEMGGNAKDPGRVMINFETPQVKEEFDRIKSLGGTVVKDPYEMGSGFIA
                     TLADPDGNYFQLMNPMP"
     gene            complement(2445..3455)
                     /gene="ansA"
                     /locus_tag="PLT08_07660"
     CDS             complement(2445..3455)
                     /gene="ansA"
                     /locus_tag="PLT08_07660"
                     /inference="COORDINATES: protein motif:HMM:NF006998.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004067 - asparaginase activity [Evidence
                     IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HNE04379.1"
                     /translation="MKKRVYIIYTGGTIGMQPTPEGYAPAPGYLAEQLLSMPELKNGL
                     MPDVDIHEYDPLLDSANMTPEDWYKIAHDIGEHYNGYDGFIILHGTDTMAYTASALPF
                     MLEGLAKPVVLTGSQIPLCEVRNDARANIITALTIAANFNIPEVCLCFGTQLLRGCRS
                     VKVSTDGLDAFASPNLPPLGEIGVEIRVNKNLVLPAPQSKLQVQELKQVNVGALPLWP
                     GIPAQVVRNFLQPPLQGLILRAYGVGNGPTNNADFLAALEEATVRGVVIVDCTQCLRG
                     SVHLGDYATGSALARAGVISGFDMTAEAALAKLSYLFSQNLPVDEIKRLMQMNLRGEL
                     TV"
     gene            complement(3543..>3849)
                     /locus_tag="PLT08_07665"
     CDS             complement(3543..>3849)
                     /locus_tag="PLT08_07665"
                     /inference="COORDINATES: protein motif:HMM:NF012958.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="alpha/beta hydrolase-fold protein"
                     /protein_id="HNE04380.1"
                     /translation="SVYTGLRMPDIFSRVLCQSGVFELEGRDFSAVDLIRARKAQELN
                     LWMDIGEYDSLLEDNRRMQPIFREHGYDVNYHEFSGGHNYTCWRDDVSHGLELLFGD"
BASE COUNT          948 a          928 c         1046 g          927 t
ORIGIN      
        1 caaatctatt tgaagcgtga agacttggcg cacacaggcg cgcacaagat caataatgcg
       61 ttggggcagg cgttgttggt gaagcgcatg ggcaagaagc gcatcgttgc cgagactggg
      121 gcagggcagc acggcgtcgc atctgccacc gctgcggcat tgctcggact cgaatgcgtg
      181 gtctacatgg gctccgtgga cattgcacgg caggagccga atgtcttccg catgaagttg
      241 ctcggcgcgg aggtgcgccc cgtggagtcg ggcaccaaga ccttgaaaga cgccatcaac
      301 gaagccatcc gtgattgggt gacgaacgtg cgcgacacgc attatctttt gggttctgcg
      361 ttgggaccac acccataccc aaccatcgtg cgcgagtttc agtcggtgat tgggcgtgag
      421 gcgcgtgagc aaatgctcat ggacgcggga cgcctgcctg atacggtcat cgcctgtgtg
      481 ggcggcggct cgaatgccat cggcgtcttc agtggatttg taaatgacga gcatgttgaa
      541 ctcatcggcg tcgaagcagg tggcagcgga atcgcttcgg gcaagcacgc ggctcgtttt
      601 ggcgacccag aaaaagcgcg agttggagtc attcacggta cacgcacgta tgtcctgcaa
      661 gatgaagatg gtcaaattgc tgaaacgcat tctgtttccg ctggtttgga ttacgccgct
      721 gtcggacccg aacacgccat gatgagagac aacgaacgcg ccttctacac ctccgcgacc
      781 gatgaagaag cattgaacgc ctttcagatg ttgtgtcaca ccgaaggaat tatccctgcg
      841 ttggagtcat cccatgcggt agcagaagtc atcaagaaag cgccgaccat gcgtaaggat
      901 caggtaatct tggtcaatct ttcgggacgc ggtgacaaag acttgaatac ggttatcaag
      961 gaattaggta attagggatt aggaattggg ttcaacgcct aattacctaa tccctaattc
     1021 ctaatctcga atatcgagga acacatgaac agacttgaat ctgcttttgc aaacaaacct
     1081 atctttatgc cttacttccc gctgggctac cctgacgtgg aaacatccgt tgacgtaatc
     1141 gaagccctcg ctaaaaacgg cgcggacttg atcgaagttg gtctttcctt ttctgacccg
     1201 cttgccgatg gacctgtaat tcagcaggct acacaagtag cgctggaaaa gggtatcacg
     1261 gtcaagaaat tgcttgcagc ggtgaaagaa cttcgcgcac gcggcgtaac catcccgctg
     1321 attttgatgg gctattacaa cccgatgctg gcgtatggac ttgagaaatt catccacgat
     1381 gcccgagagg caggtgcgga cggattcatc atccccgatc taccgttgga agaagcagag
     1441 gaattccaat ccatcaatgg cgatatgcca ttgatccaga tgctcgcgcc gacctcgccc
     1501 gatgagcgca tggaagcgat tgcccgcaat gcgaagggat ttatttatct agtttcggtc
     1561 acaggcgtaa cgggtgaacg cagagaaatc tcaaacgacc ttggcgcatt gattgctcgc
     1621 gtgcgggctc atacgtccgc gccggtttgt gtagggtttg gtattggcac acctgagcag
     1681 gcgcagcagg tcggtcaact cgcggatgga gtcattgtcg gctcggcgtg cgtgaagacg
     1741 attgggaaaa gtatgaatcc agtagaaaca gcaagacaat tttccagaga atttaaggat
     1801 gctctaaaat aatggaatgt gatataaatt tttaagggcc tgtaataaaa aatacctctc
     1861 gccatgagag gtatttttta ttcaacacat atcagcttaa ggcatcggat tcatcaattg
     1921 aaaatagttt ccatcggggt cagccagggt ggcgatgaat ccgctgccca tttcatacgg
     1981 atctttaacg accgtgccgc caagggactt gatgcgatca aattcctctt tgacttgagg
     2041 ggtttcaaaa ttgatcatca cacgcccagg gtcttttgcg ttgcctccca tttcggaatg
     2101 ggtcaggatt cccacaaagc cacttccaac ctgccatccc caaaagccat tttcctgatc
     2161 ggccatgtct gcgggctttc caaatacttt ttcatagaaa gccgccaaca cagctggttg
     2221 cattgtgctt atcattactg aattcaaatt taacatttta cgcatccttt ctaaagtagg
     2281 tcaacacgat cattaataaa acgttgaatt cattgtagaa cgaatattcc attttgacaa
     2341 gtgcgcggta tgaaaatgat cggaagtagt gataagccca ttgaaacagc gaaacagttt
     2401 gcggcggagt ttcgaagcgc gttgtctatg ttgtggaaat aatatcagac ggtcagttcc
     2461 ccgcgcagat tcatttgcat caggcgcttg atttcatcca ccggaagatt ttggctgaat
     2521 agataagata gcttggcaag agcggcttcg gcggtcatgt cgaagccgct gataactccg
     2581 gctcgggcta aggcggagcc tgtggcgtag tcaccaaggt ggacactacc cctgagacat
     2641 tgtgtgcaat ctacaatgac cacgccgcga acagtggctt cttcgagcgc ggcgagaaag
     2701 tccgcgttgt tggtgggacc gttgcccacc ccataggcgc gtagaattaa tccttgcagc
     2761 gggggttgta gaaaattgcg tacgacctgc gcgggaatgc cgggccacaa aggcaatgca
     2821 cccacgttga cttgtttcaa ctcttgcact tgcaatttgc tttgtggcgc gggcaagact
     2881 aaatttttgt tgacgcgaat ctccacgccg atttcgccaa gcggcggcag gttgggggag
     2941 gcgaaggcat ccaagccatc ggtgctgact ttgactgagc ggcatccgcg caggagttgg
     3001 gttccaaagc ataagcagac ttcggggata ttgaaattag cggcgatggt caatgctgtg
     3061 atgatgttgg ctcttgcatc gttgcggact tcgcacaatg ggatttgtga acctgttaga
     3121 acaacgggtt tagccagacc ttccaacata aacggtaacg cagaagccgt atatgccatc
     3181 gtgtcggtgc catgcaaaat gatgaagccg tcataaccgt tgtaatgttc cccaatatca
     3241 tgagcgattt tgtaccaatc ttcaggggtc atgtttgctg aatcgagcag cggatcgtac
     3301 tcatggatgt ccacatcggg catcaggccg ttctttaact ccggcatcga aagaagttgt
     3361 tcggccaggt aacccggcgc tggtgcatat ccttcaggtg taggctgcat gccgatggtc
     3421 ccgccggtgt agatgatgta gactcgtttt ttcatgggtg agttccctat gtaggaataa
     3481 aaggttcagg ggagaggata gttaatgggt ttggttaatt ttcggcggag tctgaagcgg
     3541 attcaatctc caaataatag ttccaggcca tgcgagacgt cgtcgcgcca gcaggtgtag
     3601 ttatgtccgc cactgaattc atggtaattg acatcatagc catgttctcg aaaaatgggc
     3661 tgcatccggc gattatcttc cagcagtgaa tcgtattcac cgatgtccat ccagagattc
     3721 aattcctgcg ccttacgcgc gcggatcagg tccacggcgg agaagtcgcg gccttcgagt
     3781 tcaaagactc cagactgaca cagcaccctg ctgaagatat caggcatgcg cagcccggta
     3841 tagacagac
//
LOCUS       DAOQJE010000243         3845 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_14682_length_3845_cov_6.128760, whole genome shotgun sequence.
ACCESSION   DAOQJE010000243 DAOQJE010000000
VERSION     DAOQJE010000243.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3845)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3845)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3845
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14682_length_3845_cov_6.128760"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(84..467)
                     /locus_tag="PLT08_07670"
     CDS             complement(84..467)
                     /locus_tag="PLT08_07670"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: protein motif:HMM:NF012306.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="retropepsin-like aspartic protease"
                     /protein_id="HNE04381.1"
                     /translation="MPKYDAENFDPPAPVAHVTLRDPETGASVSNVPMLLDTGADVSI
                     IPGDVLEELGASPSEGETYEVQGFSGDSQMIKAVTLEMTFLGKRFTGQFLVAEQPMGI
                     LGRNILNSVSILFDGSHLKWDEQKR"
     gene            complement(471..668)
                     /locus_tag="PLT08_07675"
     CDS             complement(471..668)
                     /locus_tag="PLT08_07675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE04382.1"
                     /translation="MTLTTYEGIVEKGKVRLKKGIRLPENAIVYVIVPEQAEKKIARV
                     RTPKLVNRKQAGQFKMKVSKA"
     gene            complement(649..1095)
                     /locus_tag="PLT08_07680"
     CDS             complement(649..1095)
                     /locus_tag="PLT08_07680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE04383.1"
                     /translation="MTMLFIGNGMEINQTTGEITKYYFAGTQRIALRKYTIPQSMSVE
                     YFLGDHLGSTSITTDTAGAKVSEMRYKPLGEVWKASQTTTPAYALTRFTFTGQYSHII
                     PVGRLLRAGNLSTEPHRGVLSDLRMKSLPIKSAIIWVEVQYDSDNI"
     gene            complement(1171..1725)
                     /locus_tag="PLT08_07685"
     CDS             complement(1171..1725)
                     /locus_tag="PLT08_07685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE04384.1"
                     /translation="MSTKEHDALVIKDMLSHAVGEKVWGVRLVAERAHRISIHFGSPT
                     PVEGKSKTVRGKWRLLTEWCVWRLEKDGDPFVGSGDVPHLPLSDQDSQEYRNEIVAMQ
                     SEVQTQLERLNNLTLLAIEVFPLSFDVTFTFSEGIVLHLFSIVSKDDESVNRRARHWF
                     FHLPNDEVLTVGPGTSWEVMKRSA"
     gene            complement(1746..2192)
                     /locus_tag="PLT08_07690"
     CDS             complement(1746..2192)
                     /locus_tag="PLT08_07690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE04385.1"
                     /translation="MEILTPLVGAKAWGPRVLVGSFIHLEFGEPRPYERDTSIMRGNW
                     VLLTQLCEWRLEKDGNLLIGSENLRENINVALQELNGSILQKVDVQLPTLDTTLFFMN
                     GVILRIFTVSFLRDNMHWVLYLPEDYQVLEVGPGKTWRLINDANAK"
     gene            complement(2235..3077)
                     /locus_tag="PLT08_07695"
     CDS             complement(2235..3077)
                     /locus_tag="PLT08_07695"
                     /inference="COORDINATES: protein motif:HMM:TIGR03696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
                     /protein_id="HNE04386.1"
                     /translation="MGFTSITTDTAGVKVSEMRYKPWGEVRYTWKNSDPAVIPSAYAL
                     TRYTYTSQYSYMDDPSTSATEGFGLMFYNARWYDPALGRMAQADSVVPAGVQGYDRYA
                     YNFNNPIKYTDPSGHDPILAGALALLTFDAVATTAAELGVAALAVVLAPEFLIAVGVG
                     LIVAGAVIAYETYTAAEEDNSEDTGITEDGSKGNWEDSEDMDDYGDWGEDGGGTQKMN
                     PDKSSNTAENETANYVRKQLEKDLGIEIDRQEFHDLVSGQGLDKEGMYQEGLNKYYTG
                     QNGQ"
     gene            complement(3204..3545)
                     /locus_tag="PLT08_07700"
     CDS             complement(3204..3545)
                     /locus_tag="PLT08_07700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE04387.1"
                     /translation="MKAIAIVSSVLYVLIIFFVSSNSLDLRKKSGWLYGMVSFLCGFA
                     LVYGLNGKVIDSAIVGIGFVFFILFNAMNERRHKREYERDTKTLIKEYGENKTFFGRL
                     VKKISVFLRKD"
BASE COUNT          979 a          975 c          796 g         1095 t
ORIGIN      
        1 cgggatggtg tacttgcgct gggcgatccg cgtcgccccg gcaaaggaat atttgtttac
       61 attatttttt ttgtacaggc ctgttatctc ttttgttcat cccacttgag atgacttcca
      121 tcaaatagaa tggacacaga gttcaagata tttctaccta gtatgcccat tggctgttcg
      181 gccacaagaa attgaccggt aaacctcttc cccagaaagg tcatttccag cgtaactgct
      241 ttgatcattt gagaatcccc gctaaaccct tgtacttcat acgtttcgcc ctcgctcggg
      301 ctggcgccaa gttcttcaag aacatcgccg gggatgatgg agacatctgc gccagtatcc
      361 agcagcatgg ggacattgga aacggatgcg ccagtttcag ggtcacgcag agtgacatgg
      421 gcaaccggcg caggcgggtc gaagttttcc gcatcgtatt tcggcatgac ttatgccttg
      481 ctgaccttca ttttgaattg acctgcctgt ttacggttca ccagtttcgg cgtgcggact
      541 cgcgcaatct tcttttcggc ttgttccggt acgatcacgt acacgatcgc attctccggt
      601 aggcggattc ccttttttag ccgaaccttg cctttttcaa caatgccttc atatgttgtc
      661 agagtcatat tgcacctcaa cccaaattat agcagacttg ataggcagtg atttcattct
      721 cagatctgac aggactcccc tatgaggttc ggtggaaagg ttcccggcgc ggagcaatct
      781 ccctacgggg atgatatggg aatattgtcc cgtaaacgta aagcgggtca gggcatacgc
      841 cggtgtggtg gtctgactcg ccttccacac ttcgcctaac ggtttgtatc tcatctccga
      901 gaccttcgcg cctgctgtat cggtggtgat ggaggtggac cccaaatggt ctcccaggaa
      961 atattccacg ctcatgctct gggggatggt gtatttccgc agggcaattc tctgtgttcc
     1021 agcaaaataa tatttggtga tttcgccggt ggtttgattg atttccatgc cgttgccgat
     1081 gaagagcata gtcatgccgt tttcggagtt ctatatttgg cgaggactac gatcagtgtg
     1141 cggcgcattg ctgggcgaga tctattcggc ttacgcgctg cgcttcataa cttcccatga
     1201 tgtgcctggc cctacagtga gtacttcatc atttggtaaa tgaaaaaacc aatgtcttgc
     1261 gcgccgattc acagattcgt catccttgga aacaatagag aacagatgta atacaatccc
     1321 ttcgctaaaa gtaaatgtta catcaaacga cagaggaaat acctctatcg caagcaaggt
     1381 caaattgttc aagcgttcaa gttgggtttg aacctctgat tgcattgcaa caatctcatt
     1441 tctgtattcc tgactgtctt gatctgataa aggcaaatgc ggaacatctc cggaccccac
     1501 aaaagggtcg ccatcttttt ctaatcgcca cacacaccat tcagtaagca atcgccattt
     1561 tcccctaacc gtttttgact tcccttccac tggtgtgggc gatccaaaat gaatagatat
     1621 acgatgcgcc ctctcggcga ctaatcgtac tccccaaacc ttttcaccga cggcatgaga
     1681 aagcatatcc ttgattacaa gagcgtcatg ttcttttgtt gacattaaaa ttcgcctcaa
     1741 taaggttatt tggcattagc atcgtttatt agccgccagg ttttccctgg tcccacttca
     1801 agtacttgat agtcttcggg caaatacaag acccaatgca tattatctct cagaaaggaa
     1861 actgtaaata ttcgcaaaat aacgccattc ataaaaaaca aagttgtatc cagcgtaggc
     1921 agctggacat ccaccttctg caagattgac ccatttaatt cttgcaaagc gacgttaatg
     1981 ttctcccgaa gattctccga gccgattagc agatttccat ccttctccag cctccattca
     2041 cacaactgcg tgagtagtac ccaatttccg cgcatgatac tagtatcccg ttcatagggc
     2101 ctcggctcgc caaattcaag atgtatgaaa ctcccgacaa gcactctcgg cccccatgct
     2161 ttcgcgccta ctaaaggggt cagtatttcc ataatgttct tctcaagtat cgtcattgta
     2221 atatcaagcc tttcctattg tccgttttgt ccagtatagt acttgttaag gccttcttga
     2281 tacatacctt ctttatcaag accttgccca gatactaaat catggaattc ttgtctatct
     2341 atttcgatgc cgaggtcttt ctcgagttgt tttctaacat aattcgcggt ttcgttctcg
     2401 gccgtattgg atgatttgtc gggattcatc ttctgtgtcc ctccaccgtc ttcgccccaa
     2461 tctccataat catccatgtc ttcactatct tcccaattgc ctttgctacc atcctctgtg
     2521 atacccgtgt cttcactgtt gtcttcctcg gcggcagtat aggtttcata tgctataacg
     2581 gcgcctgcta ctattaaccc gaccccaaca gcaataagaa actcaggagc aagaacaacc
     2641 gcaagtgccg caacaccaag ttctgctgca gtcgtagcaa cagcatcaaa agttaacagt
     2701 gctaatgctc cagctaaaat tggatcatga cctgatggat cagtgtattt gatagggtta
     2761 ttaaaattat aggcatatct atcatacccc tgcaccccgg cagggacaac gctatccgcc
     2821 tgcgccatgc gtcctaacgc agggtcatac cagcgagcgt tgtagaacat caacccaaac
     2881 ccttccgttg cggacgtgga cggatcgtcc atgtaggaat attgcgaggt gtaagtgtac
     2941 ctcgtcaacg cataagcgga cggtatgacc gcagggtcgg aattcttcca cgtatagcgc
     3001 acttcgcccc agggtttgta tctcatctcc gagaccttta cgcctgctgt atcggtggtg
     3061 atggaggtga aacccaaatg gtctcccagg aagtattcct cgctcagtta tgtcaaggcg
     3121 catcattacg ggacagagta gaagacggac taaacttacg gtttggttcg tcttttgtaa
     3181 agtagtgcat ctgtctttaa aggttaatcc tttctcaaaa atactgaaat cttctttact
     3241 aaccgtccaa aaaaggtttt attttcacca tattctttta tcaaagtttt agtatctctt
     3301 tcgtactccc ttttatggcg acgttcattc atggcgttga ataatataaa aaaaacaaac
     3361 ccaattccta ctatagctga atcaatcacc tttccattta atccgtatac aagtgcgaag
     3421 ccacaaagga aactaaccat cccatacagc cacccggatt tttttcttaa gtcaagagaa
     3481 ttagaggaaa caaaaaagat aatcagcaca tacaatacac tactaactat agctattgct
     3541 ttcatatgag aaatatcctt taggggcaag actttattat cggcttatca ttaagaaatg
     3601 atgtaatatc attaaaataa tcatgattgt atcccgcccc gtatccatca ataatgaatt
     3661 caacaacagg atcttttatt aggagcccgg cgaggtgatc gtgtccgcct gcgcaaaccg
     3721 tcccaacaca gggtcatacc agcgggcatt gtccctacgg gagacttcgc actagaacat
     3781 caacccaaat ccctcggtga ctccgcttgt gctgggatca tccatacccc ctacggggat
     3841 gatat
//
LOCUS       DAOQJE010000244         3844 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_14685_length_3844_cov_6.042228, whole genome shotgun sequence.
ACCESSION   DAOQJE010000244 DAOQJE010000000
VERSION     DAOQJE010000244.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3844)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3844)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3844
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14685_length_3844_cov_6.042228"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..949)
                     /locus_tag="PLT08_07705"
     CDS             complement(<1..949)
                     /locus_tag="PLT08_07705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016292173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HNE04388.1"
                     /translation="MNYLPSTSRYQSMHYRRSGRSGLKLPAVSLGLWHNFGGVDVYEN
                     SRAMVLRAFDLGITHFDLANNYGPPPGSAEETFGQIMAKDLHPWRDELFISSKAGYYM
                     WEGPYGEWGSRKYLVSSLDQSLKRMGLEYVDLFYHHRPDPDTPLEETMQALDFIVRSG
                     RALYVGISNYPADKAREAAQILRSLGTPCLIHQPKYSMFERWVEDGLLQTLKEEGVGC
                     IAFSPLAQGLLTDKYLGGIPEGSRASKAHGFLKPAHITDERIAKVQKLNALAQQRGQT
                     LAQMALAWVLRHEGMTSVLIGASKVSQIEDAVGMLNKVEF"
     gene            complement(1014..2045)
                     /locus_tag="PLT08_07710"
     CDS             complement(1014..2045)
                     /locus_tag="PLT08_07710"
                     /inference="COORDINATES: protein motif:HMM:NF013530.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde reductase"
                     /protein_id="HNE04389.1"
                     /translation="MTISSKPILVTGASSFVGIHTILQLLEQGYTVRGTIRSLSKETE
                     VREVVTKYGQANDRLEFKAADLEQDSGWEEAMNGIDYVLHIASPFPLLEPENEDDLII
                     PAVQGTLRVLRAAHKAGVKRVVQVSSVAAISSGYKDKIRTFTEEDWSIIENEIGAYAK
                     SKTLAERAAWDFINGPENSHHMELVAINPPLILGPVPNKNFRTSVEVIRTYMLGQVPG
                     VGRIKMGLVDVRDVAAAIILAMATPEAAGQRFLCSGGMAWLKEIAGVLHAEFSPRGYK
                     IPTLEFPSWAVRFLGLFDKKIARVTEELDRDTEQSSEKAKRILKWSPRPLKEAIIAMG
                     NSLIEHGFV"
     gene            complement(2047..2838)
                     /locus_tag="PLT08_07715"
     CDS             complement(2047..2838)
                     /locus_tag="PLT08_07715"
                     /inference="COORDINATES: protein motif:HMM:NF014049.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF92 domain-containing protein"
                     /protein_id="HNE04390.1"
                     /translation="MQLFLGFLLALLIAFLAYKARSLNKSGAIAAAFTGTIIFGIGGL
                     NWAILLLIFFITSSALSRAFKNRKAKLEEKFSKGHERDAGQVFGNGGIATLFAALHFF
                     YPNEVWVWAAFAASLAAVNADTWATELGVLNPHPPRMITNLTKQVEKGTSGGISLVGT
                     LASLAGSALIAFFASLLTSNWSLFLLVTIAGLAGSLFDSFLGGTVQAMYFCPTDQKET
                     EKHPLHTCGTQTVHIRGWKWLDNDLVNFSCGTFGMLVSLLLLGIF"
     gene            complement(2898..3806)
                     /locus_tag="PLT08_07720"
     CDS             complement(2898..3806)
                     /locus_tag="PLT08_07720"
                     /inference="COORDINATES: protein motif:HMM:NF022338.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PH domain-containing protein"
                     /protein_id="HNE04391.1"
                     /translation="MNTGKFPPPKQRGLLIHGIILFVLIVVVTIGFIQLTSADVGPAF
                     LTWLLVSLAAFAPIPFFGYRIFALYRAEYEIDRDSLSISWGLRVEDIPLSDIEWIRPA
                     DDLTNPLSLPALPLPGALLGMRRHSDLGAVEFIAADPKKLLLVATAKRVFVISPDNPA
                     GLAQTFARATEMGSLAPTEAKSVYPSFIVSQAWESGMARYLWLSALFLNIGLFVWASL
                     IIPSTPQVALAVQATGNAIETIPSSQLIIFPVASLLLAVAGWIAGLYFYRWDRERVLA
                     FIVWGSSTFTSLLFLLAVLFIVTTPV"
BASE COUNT          996 a          906 c         1056 g          886 t
ORIGIN      
        1 aaaattccac tttattcaac atcccaacgg catcttcgat ctggctgacc ttgctagcgc
       61 cgatcaacac ggaggtcatg ccttcatggc gcagaaccca agctagtgcc atttgcgcaa
      121 gggtttgtcc gcgctgctgg gcgagggcgt tgagtttttg caccttggcg attctttcat
      181 cagtgatgtg agctggtttg aggaagccat gtgcttttga agcacgcgaa ccttcaggga
      241 tgccgccgag atatttatca gtgagtaagc cttgagcgag aggtgagaag gcgatacagc
      301 caactccctc ctctttcaac gtctgtaaca gaccatcttc cacccagcgt tcgaacatgc
      361 tgtacttcgg ctgatggatc agacaaggcg tgcccagtga tcgtagaatt tgagctgctt
      421 cacgcgcttt atcagcgggg taatttgaaa tgcctacgta caatgcccgt ccactgcgga
      481 cgatgaaatc gagcgcttgc atcgtttctt caagcggagt atcagggtcg gggcgatgat
      541 gatagaacaa gtccacatat tccagtccca tgcgtttgag gctttgatca aggctggaga
      601 cgagatattt gcgcgaaccc cattctccgt atggaccttc ccacatgtaa tacccggctt
      661 tcgatgagat aaataattca tcgcgccagg gatgcaggtc ttttgccatg atctgcccaa
      721 aggtctcttc tgccgagccg ggaggcggac cataattatt ggcaaggtca aagtgagtga
      781 taccgaggtc gaaggcacgc agcaccatgg cgcggctgtt ttcgtacacg tccacgccgc
      841 cgaagttatg ccacaaacca agtgagacag ccgggagttt tagtccgctg cgccctgaac
      901 ggcggtagtg catggattga taacgagagg tggaagggag gtaattcata aaggctccta
      961 ttatataaag gtgacagtca cttttaaagt aactgtcacc tcatcttaaa attttataca
     1021 aacccgtgct cgatcaaact atttcccata gcgataattg cctctttcaa tggtcgcgga
     1081 ctccatttga gaatgcgttt cgctttttca ctcgattgtt ctgtatcgcg gtctaattct
     1141 tcggtgacgc gcgcgatctt tttatcgaac aagcctaaaa agcgcacagc ccaactcgga
     1201 aattcaaggg tagggatttt atatccgcgc ggcgagaatt cagcatgcaa aacacctgca
     1261 atctctttta gccatgccat gccgcctgaa cataagaaac gctgccctgc ggcttcaggc
     1321 gtggccatgg ctaggataat agcagccgcg acatcgcgca catctaccaa ccccattttg
     1381 atgcgcccca caccagggac ttgccccagc atgtaggtgc ggatcacttc tacggacgtg
     1441 cggaaatttt tattcggcac gggtccaagg attagcggcg ggttgatggc gaccaactcc
     1501 atgtgatggc tgttctcagg tccgttgata aaatcccatg cggcgcgttc agcgagagtc
     1561 ttgcttttgg catacgcgcc gatctcgttc tcgatgatcg accaatcttc ttcggtgaag
     1621 gttctgattt tgtctttata tcctgaagaa atggcagcaa ccgacgacac ttgcactaca
     1681 cgtttgacgc cagctttgtg cgcagctcgc aagactcgca gtgtcccttg cacggcaggg
     1741 atgataaggt catcttcgtt ttcaggctct agcaaaggaa aaggcgacgc aatatgcaga
     1801 acataatcga taccgttcat cgcttcttcc caacctgagt cttgttccag gtccgcagct
     1861 ttgaactcca gtcggtcatt ggcttgaccg tatttggtca ctacctcgcg gacttcggtc
     1921 tcttttgata gagaacgaat tgtcccacgc acggtatagc cttgttcaag caattgcaag
     1981 atggtatgaa tgccgacgaa actgctcgcg ccggtgacga gaataggctt ggatgaaatt
     2041 gtcatgttag aagattccta agagaagtaa tgagactagc attccaaacg taccgcaaga
     2101 gaaattgact aagtcattgt ccagccactt ccagccacgg atgtgaaccg tctgtgtgcc
     2161 acaagtgtgg agcggatgtt tctcggtctc tttctggtcg gtggggcaga agtacatggc
     2221 ttgaacggtc ccgccgagga aggagtcgaa gagagaacca gccaacccgg cgatagtaac
     2281 caagagaaac agcgaccagt tcgaggtcaa gagagaagcg aagaaggcga ttaacgctga
     2341 accggctaga gaggcgagcg tgcctaccag cgagattccg cctgaggtgc ctttttccac
     2401 ttgtttggtg aggttggtga tcatgcgcgg cggatgggga ttcagcacgc caagctcggt
     2461 tgcccatgta tcggcattga cggcggcaag tgaagcggcg aaggctgccc atacccaaac
     2521 ttcattgggg taaaagaaat gtagtgcagc gaagagtgtg gcgatgccac cattgccgaa
     2581 gacttgtcct gcatcacgct cgtgcccctt ggaaaatttt tcttcaagct tggctttgcg
     2641 atttttgaag gcgcggctca aggctgaaga ggtgatgaag aagatgagca agaggatcgc
     2701 ccaattcaga ccgccgatgc cgaagatgat ggtgccggtg aaggcagcgg cgatggcgcc
     2761 gcttttgttg aggctgcggg ctttgtatgc aagaaatgca atgaggagag cgaggagaaa
     2821 accgaggaag agttgcatga tgagagttgg aaagttagga tcggtttgaa ggctttaact
     2881 tgcaaacctt ctaactttta tacaggggtc gtaacaatga acagaacggc gagcaaaaac
     2941 aataaactgg tgaaggtgct ggagccccaa acaataaaag cgaggacgcg ttcacggtcc
     3001 caacgataga agtaaagacc agcaatccaa ccagcgacgg caagcaagag gcttgctacg
     3061 ggaaaaataa ttagctgcga agagggaatg gtttctattg cgttgccagt agcttgtacc
     3121 gcaagcgcca cctgtggcgt ggatgggatg atgaggcttg cccaaacgaa tagaccgata
     3181 ttgagaaaaa gtgctgaaag ccagagatac cgcgccatac cgctttccca ggcttgcgaa
     3241 acgatgaacg aaggatagac agacttggct tcagtagggg caaggctgcc catttcggtg
     3301 gcgcgggcga aggtttgtgc aagaccagcg gggttatcgg gtgaaattac gaaaacccgt
     3361 ttggcagtgg caaccagtaa aagcttttta gggtcagcgg cgataaattc cacagccccc
     3421 agatcagaat ggcggcgcat gccaagtaat gcaccgggca atggcaaggc gggcaagcta
     3481 agcggattgg tgagatcgtc tgctgggcga atccattcga tatctgaaag aggaatgtct
     3541 tcaacacgta agccccaact gatggagagg ctatcacggt cgatttcata ttctgcgcga
     3601 tacaaggcaa agatgcgata gccaaaaaat ggaatcggtg caaacgctgc cagcgaaact
     3661 aacaaccaag tcagaaatgc tggtcctaca tcagctgagg tgagttggat gaagccaatc
     3721 gtgaccacga cgataagaac gaagaggata atgccgtgaa tgaggagtcc gcgttgtttg
     3781 ggcggaggga atttaccagt gttcatgaaa ggtatagcat cttaatgaag accgttttgg
     3841 gttt
//
LOCUS       DAOQJE010000245         3843 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_14690_length_3843_cov_5.373812, whole genome shotgun sequence.
ACCESSION   DAOQJE010000245 DAOQJE010000000
VERSION     DAOQJE010000245.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3843)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3843)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3843
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14690_length_3843_cov_5.373812"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..372
                     /locus_tag="PLT08_07725"
     CDS             <1..372
                     /locus_tag="PLT08_07725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE04392.1"
                     /translation="ASLGKTLFKQSISLYGFDVSDTPNIEKADTYASLSAALDTHFSA
                     FTSNQTEAQQILNPWTQQPLADWGVKYPLQQFQSNARFSQLVVMFNPKGIYIATLGTF
                     PEDKLEEITRLGVELVKAHGS"
     gene            478..909
                     /locus_tag="PLT08_07730"
     CDS             478..909
                     /locus_tag="PLT08_07730"
                     /inference="COORDINATES: protein motif:HMM:NF033749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin family protein"
                     /protein_id="HNE04393.1"
                     /translation="MYTPKPLKWDDALSTGNQTLDEQHKYLIDTLNSLGDAIIEGHGI
                     DAIARILGRLRFYAGWHFGREEECFESYHCPAAEKNKKAHAVFIEKFDKFQDKFHESG
                     GSLDLALKVHEEISDWIVNHIMIVDGELYPCIHNHPKPLKK"
     gene            complement(951..1223)
                     /locus_tag="PLT08_07735"
     CDS             complement(951..1223)
                     /locus_tag="PLT08_07735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE04394.1"
                     /translation="MQTDPASLDPEERLNKFYALLSAGFGVLSLCAGLIPMAGTALGI
                     AGIVFGLLGRRSENKRLAVVGIILSIIGVLTAIIYSILLYRAKYGA"
     gene            complement(1226..3265)
                     /gene="uvrB"
                     /locus_tag="PLT08_07740"
     CDS             complement(1226..3265)
                     /gene="uvrB"
                     /locus_tag="PLT08_07740"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HNE04395.1"
                     /translation="MEFKLRAPFQPMGDQPEAIRGLVDGVNRGLKHQVLLGATGTGKT
                     FTIASVIQQLQKPALIMAHNKTLAAQLYAEFKEFFPENAVSYFVSYYDYYQPEAYVPR
                     HDLFIEKETEINEEIERLRLAATTALISRRDVIIVASVSCIYGLGSPEEFGKGTVTLK
                     VGEIYRRNALLRQLIESQYQRNDMELRPGTFRVRGDTLEIIPAYEDKKGYKITFFGDE
                     VERIMQFSPLTGEIYEEPQEISIYPAKQFLTDQERLKESIKNIETELEERLALYKSEG
                     KFLEAQRIEQRTRYDLEMLKEVGYCSGIENYSRHLDGRPAGSHPWTMIDFLPSDYLLI
                     LDESHMTVPQIRGMYNGDRARKETLVEYGFRLPSALDNRPLKFDEFEQVMGSTIYTSA
                     TPADYEMKHAEQVVEQIIRPTGLIDPEVVVRPVKGQVDDLVGEIKQRVERGERILVTT
                     LTKRMAEDLTTYMKELGIKVNYLHSEVETLERVGILRDLRLGNFDVLVGINLLREGLD
                     LPEVSLVAILDADKEGFLRSDTALIQTIGRAARNVNGRVIMYADRMTDSMKRAIDETN
                     RRRAKQVKYNEEKGIVPFSITKAIHDLTEEMSAKAVGEMKGEYKVKNKNEMPRNELRQ
                     VMHEMEKQMKDAAKNLEFEKAAALRDELYELKSLLAEDESLKPWERIKLLSGEEE"
     gene            3400..3843
                     /locus_tag="PLT08_07745"
     CDS             3400..3843
                     /locus_tag="PLT08_07745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE04396.1"
                     /translation="MKTSPLTPEQQNWLKANAILPVVFLVIILAVFGGIFACLFAGIH
                     ESLFFAIFFAIAGFMIVAVLAAAGMHVYNNFMDLRDGVAQVREGELTRKHHTYRSPKT
                     FYAEFEGVGSIIVMGDVYEKLEEGKTYRVIYSPRTRRGWDVDLRS"
BASE COUNT         1058 a          966 c          791 g         1028 t
ORIGIN      
        1 gcctctctcg gaaaaaccct tttcaaacag tccatttcgc tttatggatt cgacgtttca
       61 gacacaccaa acatcgaaaa agcggatacc tacgcctctc tctccgccgc gctggatact
      121 cacttctcag cctttaccag caaccaaact gaagcgcaac aaattctcaa cccgtggacc
      181 cagcaaccgt tggcagactg gggcgtgaaa tatccgttac agcaattcca atccaacgca
      241 agattcagtc aactcgtagt gatgttcaac cccaaaggca tctacatcgc tacactagga
      301 accttcccag aagacaagct tgaagaaatt acccgtcttg gcgtggagtt ggtcaaagca
      361 catgggagtt aaaaatagga caatcgtcct ccgaatttca ttacaatgat acgaagtctc
      421 cagagagacc ccggaatata atttgaatct aaatcagttg tctttctgga gaaaaaaatg
      481 tacacaccca aacccctaaa atgggatgat gcactcagta ctggcaacca aaccctcgac
      541 gagcaacaca agtacctgat cgatacccta aatagccttg gcgatgcaat tattgagggg
      601 cacggaattg acgccatcgc cagaattcta ggcaggttgc gcttctatgc tggctggcat
      661 tttgggcgcg aagaagaatg ctttgaaagc tatcactgcc cggcagccga aaagaacaaa
      721 aaagcacatg ccgtgtttat cgaaaaattc gataaatttc aagacaaatt tcacgaaagc
      781 ggtggctcgc ttgatttagc gctaaaagtc catgaagaaa tttcagactg gatcgtaaat
      841 cacatcatga tcgtggacgg cgagttatat ccttgcattc ataatcaccc caagccgctc
      901 aaaaaataat ataaaagagc cgcagaattc tgcggctctt ttcgttgtaa tcaagcacca
      961 tattttgctc gatacaaaag aattgaataa atgatggcag tcagcacccc aataattgat
     1021 aagataattc caaccactgc caaccgttta ttttcagacc gcctgcctaa taaaccaaag
     1081 acaataccgg caattcccaa cgccgtccct gccatgggaa ttaatccggc acataaactc
     1141 aataccccaa aacctgccga aagcagggca tagaacttat tgagtcgctc ttccgggtca
     1201 agagaagcag ggtctgtttg catggctatt cttcttcacc agatagtaat ttgattcgtt
     1261 cccaaggctt caggctttca tcctctgcca ataagctttt caactcatac agctcatcgc
     1321 gcagagccgc cgccttctca aactcaagat ttttcgcagc atctttcatc tgcttttcca
     1381 tctcatgcat tacctggcgc aactcgttgc gcggcatttc attcttattc ttaaccttat
     1441 actcgccctt catttcgccc accgccttgg cagacatttc ctcggtcaga tcatgaattg
     1501 ccttggtaat actaaacggc acaataccct tttcttcgtt atatttcacc tgcttggcac
     1561 ggcgacgatt ggtctcgtca atggcgcgtt tcatcgaatc ggtcattcgg tcagcgtaca
     1621 tgatgacacg tccattcaca ttacgcgccg cacgcccaat cgtttggatc aatgcagtat
     1681 cagagcgcaa gaaaccttct ttgtccgcat cgagaattgc caccagcgac acttcaggca
     1741 agtccaaccc ctcgcgcaaa aggttgatac ctaccaacac atcaaaatta ccaaggcgta
     1801 aatctcgcaa gatacccaca cgctccagcg tttccacttc agagtgcaaa taattaacct
     1861 tgatgcccaa ctctttcatg taagtagtta gatcttctgc catgcgtttg gtaagggtag
     1921 tgacgaggat acgctcgcct ctttctaccc gctgcttgat ctctccgacc aagtcatcta
     1981 cctgcccctt gaccgggcgc acaactactt cagggtctat caaccctgtt gggcggataa
     2041 tctgctcaac gacttgctcg gcatgcttca tttcataatc agctggcgtc gcagatgtgt
     2101 aaatcgtgga gcccatcacc tgctcaaatt catcgaactt gagcggacga ttatccaatg
     2161 cagatggtaa acggaaacca tattccacca atgtctcttt acgagcgcgg tcaccgttat
     2221 acatgccgcg tatttgcggc acagtcatat ggctctcatc taggatcaac aaataatcgc
     2281 tgggcaaaaa gtcaatcatc gtccatggat gtgaaccggc agggcgtcca tccaaatggc
     2341 gagaataatt ctcaatccct gaacaatagc caacttcttt gagcatttcc aagtcatatc
     2401 gagtccgttg ctcaatgcgt tgtgcttcaa gaaactttcc ttcgctttta tacaaagcca
     2461 agcgttcttc caactcagtt tcaatattct taatcgactc tttcaggcgt tcttgatcgg
     2521 tgagaaattg ctttgccggg taaatcgaaa tttcttgcgg ttcttcataa atttcaccgg
     2581 tcaacgggct aaattgcata atacgttcta cttcatcccc aaagaaagtg attttgtaac
     2641 cttttttatc ttcatatgca ggaatgatct ccaacgtatc accacggaca cggaaagtac
     2701 ctgggcgtaa ttccatatca ttgcgctggt actggctctc aattagctga cgcagtaagg
     2761 cattgcgacg gtaaatctct cctactttta gggtcacagt ccctttccca aattcctctg
     2821 gtgaacctaa accataaata caagaaactg aagcgacaat aatgacatct cggcgagaaa
     2881 tcagagctgt cgttgcagct aggcgcaggc gctcaatctc ttcattaatc tcggtttctt
     2941 tttcaatgaa caaatcatgc ctcggaacat atgcctctgg ctgatagtag tcgtaatacg
     3001 atacaaaata agaaaccgca ttttcaggga agaactcttt aaactctgcg tacaactgtg
     3061 cagctaaggt tttattatgc gccatgataa gggcaggttt ctggagttgt tgaataacag
     3121 acgcgattgt gaacgtttta ccggtccctg ttgcgccgag caagacctga tgcttaagac
     3181 cacggttcac gccatccaca agtccgcgaa tggcttcggg ctggtcgccc atcggttgga
     3241 agggagctct gagtttaaat tccataagtt tatcttttcg gtattatgtg aacgagtgtt
     3301 ctattttacc tcaacacagc caaggtgtgt ggaaactgac agccattgca caagcatggg
     3361 acaagaaatc accacgggta taatttaaag catataaata tgaaaacaag cccactcacc
     3421 cctgagcaac aaaactggtt gaaggcaaat gcgattctgc cagttgtatt tcttgtgatc
     3481 atactggctg tctttggtgg gatatttgct tgtctttttg ctggcataca tgaaagtctt
     3541 ttcttcgcta ttttctttgc cattgccggt tttatgatcg ttgcggttct tgcggcagca
     3601 gggatgcatg tatacaacaa ttttatggac cttcgtgatg gtgtggcgca agtgcgcgag
     3661 ggagaattga ctcgtaaaca tcatacctat cgctctccaa aaacgtttta cgcggagttc
     3721 gagggagtcg gttctataat cgtcatgggc gatgtttatg aaaagcttga agaaggcaag
     3781 acctatcgtg taatctacag tccgcgtacg aggcgtggat gggatgtaga tttgcggtct
     3841 taa
//
LOCUS       DAOQJE010000246         3841 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_14704_length_3841_cov_6.716587, whole genome shotgun sequence.
ACCESSION   DAOQJE010000246 DAOQJE010000000
VERSION     DAOQJE010000246.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3841)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3841)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3841
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14704_length_3841_cov_6.716587"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..988
                     /locus_tag="PLT08_07750"
     CDS             <1..988
                     /locus_tag="PLT08_07750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE04397.1"
                     /translation="KETVQERAYNDPDIVAAYLTGSLRGEPMLGGTADIDIVFVHKTT
                     PMKSREFVKLTPDFHIDISRRAKDEFKSPRELRGDPWLGYEMYDPVLLYEREKFFDFV
                     QASLRAGFEFEQPPLMLQRCRTLLNHGRGNWMDISEHDGEVGPKEIRKYMKSLFHAVN
                     AIVELSGPPIWERRLLLDFPARAEAIQKPEMITTIYGLLGATQVDAEKIKTWLNDWKH
                     AFKLASETPGVDARIHPARLNYYEKSIKALLDSDTPLSALWPVLHTWTLSAKVLSGDH
                     LKFWEQACNSLGLLEGFADKVSGLDHFLDEIEIIFEEIATANGLDVEPSTGL"
     gene            1137..1481
                     /locus_tag="PLT08_07755"
     CDS             1137..1481
                     /locus_tag="PLT08_07755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="YqfO family protein"
                     /protein_id="HNE04398.1"
                     /translation="MYDFTDVKLEIFVPETHVNYVRDALVEVGIGVIGNYDHCFAISQ
                     VQGSFRPLPGSNPFDGKIGEVTYSVESKIEVNCRRELVEEALAAVRRSHPYEEPLINI
                     FPLANHLFTRNV"
     gene            1478..2521
                     /locus_tag="PLT08_07760"
     CDS             1478..2521
                     /locus_tag="PLT08_07760"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HNE04399.1"
                     /translation="MKSKNLVYIFQALLAALFFGASAPIAKLLLGDIAPIYLASFLYL
                     GSGTGIALTKLTQPKGTEASIQRADVPWLAGAIISGGILAPIVLMNSLQNTPASTASL
                     LLNFEGVGTTLLAALFFKESISRRAWMAIIVITLASIFLSTDFSGGFELSLGALGIIL
                     ACVLWGADNNFTRNISGKDPLVIVAWKGLVAGSFSFILASSLGNPFPAVTTILGTLLL
                     GFLSYGLSTMLFIRSMRGLGAARTSALYGTAPLAGVALSIFMFGEFPSFFFIIAAILM
                     IGGALLLINEEHSHTHVHTAIVHEHSHSHDDGMHGHSSGEGVHSHGHEHPEEEHDHDH
                     MPDIHHRHGHEDK"
     gene            2588..3568
                     /gene="pyrB"
                     /locus_tag="PLT08_07765"
     CDS             2588..3568
                     /gene="pyrB"
                     /locus_tag="PLT08_07765"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase"
                     /protein_id="HNE04400.1"
                     /translation="MPTTSTNPHLPFGENKNADWYGKDIISVKQFSRDDLEYVFGVAH
                     EMRGMVERIGTFDLLKGKILANLFYEPSTRTSSSFTAAMERLGGSVIPINEVKYSSVS
                     KGESLPDTIRTLECYADVIVLRHPETGSAAIAAKAAKKPVINAGDGVGEHPTQALLDT
                     FTIMEELGRLDNLNVTMLGDLKYGRTVHSLARLLSQFSNIKLNYVSPDILKMPKEVME
                     EVGAKGVPQAEYSSLEQVLPETDVLYVTRVQKERFEDPADYEKVKGAYVIDPATMKAA
                     KKEMAVMHPLPRVGEISTDFDDDPRAAYFRQMEYGLYVRMALLAMVLGKA"
     gene            complement(3615..>3841)
                     /locus_tag="PLT08_07770"
     CDS             complement(3615..>3841)
                     /locus_tag="PLT08_07770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE04401.1"
                     /translation="EQFGYPDKPGLPIIIDQAVKETLTFSSDHSYSYEKMGFTRQGIV
                     ERYKDIFERFNFETREEEELVPVKRMELER"
BASE COUNT          933 a          899 c          943 g         1066 t
ORIGIN      
        1 caaagaaacc gttcaagaac gggcatataa cgacccggat attgttgcgg cgtatctgac
       61 tggctcattg cgcggcgaac ctatgttggg cggaactgcc gatattgata ttgtctttgt
      121 ccacaagacc actccaatga agtcacgcga gttcgtcaaa ctcacgcccg attttcatat
      181 tgatatcagc cgccgtgcga aagatgaatt caagtcgccg cgtgagttgc ggggtgaccc
      241 gtggcttggg tatgaaatgt acgacccggt tctgttgtat gagcgcgaaa aattcttcga
      301 ctttgtgcaa gccagtctgc gcgctggctt tgagtttgaa cagccgccgc tgatgttgca
      361 gcgctgccgt acccttctca atcatgggcg cggcaactgg atggacatct cggagcatga
      421 tggggaagtg ggtccgaaag aaattcgcaa atacatgaag tcgctttttc atgcggtcaa
      481 tgcgattgtt gagttgagcg gtccacccat ttgggagcgc cgcctcctgc tcgatttccc
      541 tgcccgtgct gaagccatcc aaaagcctga aatgatcact accatatatg gtttgctcgg
      601 cgccacacaa gtggatgccg agaagatcaa gacctggctc aacgattgga agcacgcctt
      661 caaactcgca tctgaaactc ccggcgtgga tgcaaggatt caccccgcac gcctgaacta
      721 ttacgagaaa agcatcaaag ccctgctgga tagtgatacg cctctttccg cgctctggcc
      781 tgttctgcat acctggacct tatctgccaa ggtgctgagt ggtgaccatc tcaagttctg
      841 ggaacaagct tgcaactcgc ttgggctact tgaaggcttt gcagacaaag tctctgggct
      901 tgatcacttc cttgatgaga tcgagattat ttttgaagag atcgctaccg ccaacggatt
      961 ggatgtagaa ccatcgacag gtttataaat gcgagtataa tcgcgccgat gtttataggg
     1021 tatccgcacc gccatttatt aaacaagttc cgcccgggcg cattctttgt tttctttcat
     1081 cgttctcaaa agcctgacaa ttaaatgtca ggcttttttc ttaatggaga tattcgatgt
     1141 acgactttac tgatgtcaaa ctcgagattt tcgtccctga aacgcatgtc aactatgttc
     1201 gcgatgcgct ggtagaagtt gggattggtg tgatcgggaa ctatgaccac tgttttgcca
     1261 tctcacaagt gcagggctcg ttccgcccat tgccggggtc gaatccgttt gatgggaaaa
     1321 taggggaggt cacttacagc gtagagtcaa agatagaagt gaattgcagg cgtgagcttg
     1381 tggaagaggc gcttgccgca gtgcggaggt ctcatcctta tgaagaacca cttattaata
     1441 tctttccgtt ggcgaatcat ctatttacga ggaatgtatg aagtcaaaga atcttgttta
     1501 tatttttcaa gctctgctcg ccgctttgtt ctttggtgca agtgcaccga tcgcgaaatt
     1561 attgctgggt gatattgcgc caatttacct tgcttccttt ttgtatctcg gcagcggaac
     1621 cggaatcgcc ctcacaaaac ttactcaacc caaaggaacc gaagccagca ttcagcgtgc
     1681 agatgtgcct tggctggcgg gtgcgattat ctctggcggt attcttgcac caatcgtcct
     1741 tatgaatagt ttgcaaaata cgcctgcatc aacggcttcg ttactgctta atttcgaagg
     1801 tgtagggact actttgttgg ctgctttatt tttcaaagag tcgattagtc gccgcgcatg
     1861 gatggcgatc attgttatta cccttgcaag tatttttctt tctactgatt tttcaggcgg
     1921 tttcgaactt tcacttggcg ctttgggtat catccttgct tgtgtacttt ggggcgcaga
     1981 caataatttc acgcgcaata tttctggtaa agacccgctc gtcatcgtag catggaaggg
     2041 attggttgct ggttcatttt ctttcatctt agcttcgtcg cttggaaatc cattccctgc
     2101 ggtgacgact attcttggaa cgctgctgtt ggggtttctt agctatggat tgagcacaat
     2161 gctgttcatc cgctccatgc gcggacttgg cgccgcccga accagtgctt tgtatggtac
     2221 ggctccgctg gcgggtgttg ctttatctat ttttatgttt ggtgaatttc cttctttctt
     2281 tttcattatc gccgcgattt tgatgattgg cggcgcgttg cttcttatta atgaagagca
     2341 ttctcacacc catgtgcata cagcaattgt tcatgaacat agccatagcc atgatgatgg
     2401 catgcacgga catagtagcg gcgaaggtgt ccattcgcat gggcatgaac accctgaaga
     2461 agaacatgac catgaccata tgccagatat tcaccatcgg catgggcatg aagacaagta
     2521 aataccgata tatgcccaac tcatatatcg aattgataac caattatcag aaccaggaga
     2581 ctaagaaatg ccaacaactt ccacgaaccc ccatttgcct tttggcgaaa acaagaacgc
     2641 cgattggtac ggcaaggaca ttatttccgt caagcaattt agccgtgatg acttggaata
     2701 tgtctttggc gtggcgcatg aaatgcgcgg aatggtcgag cgcattggaa cctttgactt
     2761 actcaaaggg aaaatccttg ctaatctctt ctacgaacca tccacacgta cttcatcttc
     2821 attcacggct gccatggagc gtttgggtgg gtcagtgatt ccgatcaatg aagtgaaata
     2881 ctcatcagta tccaaaggtg aaagcttgcc cgataccatc cgcaccttgg aatgttatgc
     2941 cgatgtgatc gttctgcgtc accccgaaac cgggtccgcg gcgattgcgg caaaagcagc
     3001 aaagaaaccg gtcatcaatg caggcgatgg ggtcggtgag cacccgactc aagccttgct
     3061 cgatacattc accattatgg aagaacttgg tcgccttgat aacttgaacg tcactatgct
     3121 cggtgacttg aaatatgggc gcactgtcca ctcgctggcg agattgctct cgcagttcag
     3181 caacatcaag ctcaattacg tctcgccaga tattttgaaa atgccgaaag aagtaatgga
     3241 agaagtgggc gctaagggcg tgccgcaagc ggaatattct tctttggagc aagtcttgcc
     3301 tgaaacggat gtgctctatg tcacccgtgt tcagaaagaa cgctttgaag accccgccga
     3361 ttatgaaaaa gtaaagggcg cttatgtgat tgacccagct actatgaaag ccgcaaagaa
     3421 agaaatggcg gtcatgcatc cccttccgcg tgtgggcgaa atctcaacag actttgatga
     3481 tgatccacgc gctgcttact tccgccagat ggaatatggt ctgtacgtga gaatggcatt
     3541 gcttgccatg gtgcttggaa aagcataaac gaggttaaat aaaaacgcgg acacttgtgt
     3601 ccgcgttttt tgttttatcg ttctaattcc attcgtttga ctggaacgag ttcttcctct
     3661 tctcgcgttt caaaattgaa gcgttcgaag atatctttgt atctctccac gattccctga
     3721 cgggtaaagc ccatcttttc ataggaatag gaatggtcac tgctaaaggt caatgtttct
     3781 ttgacagctt ggtcaatgat gatgggcaga ccgggtttat cgggataacc aaattgctca
     3841 t
//
LOCUS       DAOQJE010000247         3840 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_14710_length_3840_cov_6.375958, whole genome shotgun sequence.
ACCESSION   DAOQJE010000247 DAOQJE010000000
VERSION     DAOQJE010000247.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3840)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3840)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3840
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14710_length_3840_cov_6.375958"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..739)
                     /locus_tag="PLT08_07775"
     CDS             complement(<1..739)
                     /locus_tag="PLT08_07775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE04402.1"
                     /translation="MKKISHLLLVLLILISIPARSAHADVAPPETVPGSNLVPGGEST
                     QVRMVAETVTLTISQDPADEKRAMAETRAVFTMRNLGTAEESMAVRFPLSFFDGASDG
                     FFNYPELSSIAVKVDGKTVPTRREMQPFLASQSNSSDRDEIPWSVFDVTFPPNQDVTI
                     EVVYNVDGFGDYPYEVFKYVLETGAGWNGTIGSAEIILRFPYEANTYNVWTEGVEGYG
                     DATPGGVFSGNEVHWKYTELEPTFENNL"
     gene            894..1715
                     /locus_tag="PLT08_07780"
     CDS             894..1715
                     /locus_tag="PLT08_07780"
                     /inference="COORDINATES: protein motif:HMM:TIGR00010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HNE04403.1"
                     /translation="MQLTDTHCHLDYHKYNTDRADVIQRAKDAGLVKVLVPGLHHRSS
                     HEAVKLAESNTMVYAAVGFHPTDLDEFSEKAFLQVKELAKHPKVVAIGEIGIDYYWVK
                     EAEKRAVQREVLKQHLAFAQEVNKPVVLHMREQDDAWFGEASVDLLEILSEWQNGLRG
                     ELREKPGVLHSFNGTLETAQKALSLNFYLGVTGPVTYPSAEKKREVISQLPLDRLLIE
                     TDGPYLTPAPHRGKRNEPAYVVHIADKIAEIHSKSPAEIAQITTANAKRLFAWGD"
     gene            1717..2682
                     /locus_tag="PLT08_07785"
     CDS             1717..2682
                     /locus_tag="PLT08_07785"
                     /inference="COORDINATES: protein motif:HMM:NF012568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HNE04404.1"
                     /translation="MNSVTFLSPVTEEIKLIEDRMRIQADDSHPDLRAALKHLLAAGG
                     KRIRPTLGMLVGNMLGAPLEKLINLGAAVELLHTATLVHDDLIDGSLLRRGTPTLNAR
                     WSPPATVLTGDFLFARAAKLAADTDYLPLMKLFSETLAIIVNGELTQMFTSRGLINRD
                     NYYKRIYAKTASLFEMTSRSAAMVSPASDDSIEAMRDFGYQIGMAFQIVDDILDFNGD
                     QNAVGKPLGSDLLNGLVTLPAIYFAEINPDDPDVMSLPQGGWTNNENMTRLVEKIRAS
                     SCTKKAMVEAEQHVDRALVCLESFEPCAERDALENLAKYIVDRKV"
     gene            2749..3258
                     /locus_tag="PLT08_07790"
     CDS             2749..3258
                     /locus_tag="PLT08_07790"
                     /inference="COORDINATES: protein motif:HMM:NF014657.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HNE04405.1"
                     /translation="MTNPRKPFRFNVGFIIHEEVGYNHDFPFEFEKVTLNGDLELRNL
                     EGNVNVGKTPQGLIVQGKFTAETTVTCSRCLTDVVQDLEWEFTEMYAFDQRSITESGL
                     LLPEDAQLDVESLLREYALLEVPINPICKPDCLGLCIECGQNLNEKDCGHRPEEPDSP
                     FAKLKDLLK"
     gene            3266..>3840
                     /locus_tag="PLT08_07795"
     CDS             3266..>3840
                     /locus_tag="PLT08_07795"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021134.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 87 protein"
                     /protein_id="HNE04406.1"
                     /translation="MPPAYAGGFHFSMKLSIPKPIRILITILVLVVLIFIRVRINLGF
                     DVPNSNFSFFWLAGRMILDGQNPYDETQYLAGHDANSMDWRPNNIFPYPLPLALFCIP
                     LGFFSMQTAYVIWQIVTLLLIALTIYLLLQHWQEESQQNLLLPIFFFLLFLGPVYLST
                     HAGTISILPVMVIVGAILLLEKEQSLWAGVVL"
BASE COUNT          995 a         1001 c          880 g          964 t
ORIGIN      
        1 gaagattatt ttcaaaggtc ggctctaatt cggtgtactt ccaatggact tcattcccgc
       61 taaacacgcc accgggtgtg gcatcgccat agccttccac gccttctgtc catacattgt
      121 aggtattcgc ttcataggga aagcgcagaa tgatctcggc actgccaata gtgccattcc
      181 aaccggcgcc cgtttcaaga acatatttga agacttcata aggataatcg ccaaagccat
      241 ccacattgta tacaacctcg atagtgacat cttgattggg cggaaaggtc acatcgaaaa
      301 cagaccaagg gatttcatcg cggtctgaag agtttgattg gctggctaaa aagggttgca
      361 tctcacgccg agttggcaca gttttcccat ccacctttac ggcaatggag gaaagttcag
      421 ggtagttgaa aaagccatcc gatgcaccat cgaaaaaaga aagcggaaaa cgcactgcca
      481 tgctctcttc tgccgttcct aaattgcgca tggtgaaaac ggctcgtgtt tcagccatag
      541 ctcttttttc gtccgccggg tcttgagaga tagtgagtgt gaccgtctct gctaccatgc
      601 ggacttgggt tgattcgccg ccgggcacaa ggttcgagcc agggacagtc tccgggggag
      661 ctacgtcggc atgggcagag cgagcgggaa tagaaatcaa aatcaggaga acgagcaaga
      721 gatgcgatat ctttttcata agggacctat attgtttcct actatacaag aatgaatccc
      781 attggtcaat tcggcaaaag taccagacga atgtcaccaa tacctcactt attttgtaaa
      841 aatcatccat ttaacatttt gccctaaaaa cagactgata taatgccaac gctatgcaat
      901 tgaccgacac ccactgtcac cttgattatc ataaatacaa cacagaccgc gcagatgtaa
      961 tccaacgtgc aaaagatgca gggttggtaa aggttctggt accaggactc catcacaggt
     1021 ccagccatga agcggtgaag ttggcagagt ccaacacaat ggtgtatgcg gcagtcggct
     1081 tccatcctac agatttggac gagttttctg aaaaagcgtt cttacaagtg aaggaattgg
     1141 cgaagcaccc caaagtggtt gccattggcg agatcggcat tgactattat tgggtcaaag
     1201 aggcagaaaa acgcgccgtt caacgcgaag ttctcaaaca acatttagcc tttgcgcaag
     1261 aagtgaacaa acccgtggtt cttcacatgc gcgaacaaga cgacgcctgg tttggcgaag
     1321 cctctgtgga tttgttagag atcttgagcg agtggcaaaa cgggctacgc ggagaattgc
     1381 gcgaaaaacc cggcgtttta cactcgttca atggcacgtt agagaccgcc caaaaggcgc
     1441 tgtcactgaa tttttacctc ggtgttacgg gtccagtgac atacccaagc gcagagaaaa
     1501 aacgcgaggt gatttctcaa cttccgctcg atcggctgct cattgaaaca gacggtcctt
     1561 accttacacc tgccccacac aggggtaaaa gaaacgagcc cgcctacgtc gttcatattg
     1621 ctgataaaat agcagaaatc cattccaaaa gccctgcgga gatcgcccaa attaccactg
     1681 ccaatgccaa acggctcttc gcatggggag attgacttga actcggtaac ctttctttca
     1741 cccgttacag aagaaataaa actcattgaa gatcgcatgc ggattcaggc agatgatagc
     1801 caccctgacc tgcgtgccgc tttgaaacac ttattggcag ccggagggaa gcgcatccgc
     1861 ccgaccttgg gcatgcttgt tggcaatatg ctgggtgccc cactggaaaa gctcatcaac
     1921 ctcggcgccg ccgtagagct tttgcacact gccaccttag tccacgatga tttgatcgac
     1981 ggctcgttac ttcgccgcgg cacgcctacc ctgaacgcac gctggtctcc acctgcaacc
     2041 gtgctcactg gcgactttct ctttgcccgc gccgccaaac tcgccgcaga tacagactac
     2101 ttacctctga tgaagttgtt ttctgaaacc ttggcgatca tcgtcaatgg cgaattgacg
     2161 caaatgttca catcacgtgg gctcatcaac cgcgataact actacaagcg catttacgcc
     2221 aaaaccgcct ccctcttcga aatgacctcc cgctctgctg caatggtcag cccggcaagc
     2281 gatgactcaa ttgaagccat gcgcgatttc ggctatcaga tcgggatggc attccagatc
     2341 gtggatgata ttttggactt caatggcgat cagaatgctg tgggcaaacc gctcggttca
     2401 gacctactaa atggcttggt caccctccca gccatttact tcgctgaaat caaccccgat
     2461 gacccggatg tgatgtcgct gccccaaggc ggttggacca ataacgaaaa catgacccgc
     2521 ctcgtggaaa agatccgcgc cagtagctgc accaagaaag ccatggttga agccgaacaa
     2581 catgtagacc gggcactcgt ctgccttgaa agttttgaac cgtgtgccga acgcgatgcc
     2641 ttggaaaact tggcaaagta tattgttgat agaaaagtct aagaatcagg aatccagatt
     2701 taacagaccc tgattttcta gtttcttatt cctaatccct tatttcccat gaccaaccct
     2761 cgcaaaccct ttcgctttaa cgtcggcttc attattcatg aagaagtcgg ctataaccac
     2821 gactttccgt tcgagttcga gaaggtcact ctcaacggtg acttggaact ccgcaacctg
     2881 gaaggcaatg tcaacgttgg caaaacccct cagggattga tcgttcaagg caaattcacc
     2941 gctgaaacca cagtcacttg ttcacgttgc ctcaccgacg ttgtacaaga ccttgaatgg
     3001 gaattcaccg aaatgtacgc cttcgaccaa cggtctatta cagagtctgg tttgctgttg
     3061 cccgaagatg ctcaactaga tgtagagagc cttctgcgcg agtatgcgct tttagaagtg
     3121 cccatcaacc cgatttgcaa acccgattgt ctcggtctgt gcattgaatg cggtcaaaac
     3181 ctcaacgaga aagattgcgg tcaccgcccc gaagaacccg attcgccttt tgcaaaattg
     3241 aaggacttgc ttaagtaaaa cctgcatgcc acctgcttac gcgggtggtt ttcatttttc
     3301 tatgaaactt tccattccta agcctattcg catcctcatc acaatcctcg ttctggtagt
     3361 tttgatcttc atccgcgttc ggattaacct cgggttcgat gtccctaact ccaatttttc
     3421 tttcttctgg ctggcagggc ggatgatttt ggatggacaa aacccatacg acgagactca
     3481 atatctcgca gggcacgatg ctaacagcat ggattggcgc ccaaataata ttttccctta
     3541 tcccctaccc ttggctttat tctgtattcc gctcggcttc ttttccatgc agacggcgta
     3601 cgtcatttgg cagattgtta cgcttttgct cattgccctg accatttacc ttctgttgca
     3661 acactggcaa gaggaaagtc agcagaattt attgctgcct atatttttct tcttactctt
     3721 tttaggtccg gtctatctaa gcactcatgc agggacaatc agcattctgc ctgtgatggt
     3781 gatagtaggt gcaattctct tgcttgaaaa agaacagtcg ttatgggcgg gtgtcgtcct
//
LOCUS       DAOQJE010000248         3823 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_14798_length_3823_cov_6.522028, whole genome shotgun sequence.
ACCESSION   DAOQJE010000248 DAOQJE010000000
VERSION     DAOQJE010000248.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3823)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3823)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3823
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14798_length_3823_cov_6.522028"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..927)
                     /locus_tag="PLT08_07800"
     CDS             complement(<1..927)
                     /locus_tag="PLT08_07800"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNE04407.1"
                     /translation="MRLLFVTDARSPISKNWIRHFIERGDEVFIASTFEWDVEGIPSE
                     RTEFTPVAFSSAKKRTSAPSTASSRTLSLRTKIRQWLGPVTIPAAAFKLRAFIREVQP
                     DIVHAMRIPYEGMLTAEALQGLKARPTFLASIWGNDFTLHAPSTPLIRYYTRKVMNSV
                     DGLHADVERDVKLAHEWGLSAEKPTLVTPGNGGVRSDVFYPPKELVKSPIIINPRGVR
                     PYVRNDSFFKAIPLVLAKYPDAKFLCVGMQNEPQAMRWMQEYGIENSVELLPTSPHEK
                     MGEVFRGAQIVVSPSIHDGTPNTLIEAMACGCF"
     gene            992..1366
                     /locus_tag="PLT08_07805"
     CDS             992..1366
                     /locus_tag="PLT08_07805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNE04408.1"
                     /translation="MSAGRILIVEDNMDTYELVRFILEKNGFETFLAMNGRDGVNAAT
                     KQRPDLIIMDLSMPEMDGWTAMRLIKSDPAISSIPMIALTAHALPSDRKRAFDAGCDE
                     YITKPMDLIDLLDTVKSWIQKV"
     gene            1451..2746
                     /locus_tag="PLT08_07810"
     CDS             1451..2746
                     /locus_tag="PLT08_07810"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose/GDP-mannose dehydrogenase family
                     protein"
                     /protein_id="HNE04409.1"
                     /translation="MKQICVVGVGYVGLVTAACFADLGNRVVALDVNEERVANLKKGI
                     MPIYEPGLDELVKRNVKAGRITFTSSYTEALKGAEYAFIAVGTPSGSDGSADLQYVAA
                     AATSIAKNMTAPLILINKSTVPIGTGDWVADIVKGAQPKPIDFAVVSCPEFLREGSAI
                     GDFMNPHRTVIGSLDRDAANKVAQLHLPLRAPIVITDLRTAEMIKYASNAFLATKISF
                     INELADLCERVGADVKEVAAGMGYDARIGRHFLDAGLGWGGSCFPKDVEALAFMAKEK
                     GLEPKILNSVMDVNYDRRKEAVKVVENMVGGSLKGKTIGLLGLAFKPNTDDMRDAPSI
                     DISAELIEAGAKVRGYDPVAMSVARPILPAVEMYDEPYKLAEGCDALMIITEWNEFKQ
                     LDLDKVKSLLKSPVIYDGRNIYEPGRMKDMGFTYRAIGR"
     gene            2836..3738
                     /locus_tag="PLT08_07815"
     CDS             2836..3738
                     /locus_tag="PLT08_07815"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNE04410.1"
                     /translation="MTNTPPVCNYEGSDYQQSFWDQGGRAYEDQSEAIALRRMLPKSG
                     KLMLEVGAGAGRNTPRYAGFDRIVVMDYSTTQLAQAQERLGRSDKYIYVAADVYRLPF
                     RNGLFDGATMIRVLHHMADAPKALGQIQGTLQPGATFILEFANKLNLKAILRYWLGKQ
                     TWSPFTLEPVEFVKLNFDFHPKAIRQWLGNLGFNIEKTLTVSHFRLGLLKRTIPSKLL
                     AAVDGLFQPTGALFQLTPSVFVKAVLPGASSADIPLNVIDLFKCPECNSGGLQEKTDH
                     VFCPSCKAKWSVKDGIYDFREKIS"
BASE COUNT          991 a          939 c          943 g          950 t
ORIGIN      
        1 gaaacatcca cacgccatgg cttcgatcag cgtgtttggc gtcccatcgt gaatactggg
       61 ggaaaccacg atttgtgcac cacgaaacac ctctcccatc ttttcatgag gagaggtagg
      121 caacaactcc acagaatttt caattccata ctcttgcatc cacctcatgg cttgtggctc
      181 attttgcata cctacacata agaacttcgc atcaggatat tttgccagca ccaagggtat
      241 cgccttaaaa aacgaatcat tccgcacata gggtcttact ccacgagggt tgatgatgat
      301 tgggcttttc accaactctt ttggcggata aaaaacatcg cttctaactc cgccgttacc
      361 gggtgtcacc aacgtaggtt tctctgcact caagccccat tcatgtgcca gttttacatc
      421 ccgctctaca tctgcatgca aaccatctac agaattcatc accttgcgag tgtaatacct
      481 aatcagtggt gtagatgggg catgcagggt aaagtcattg ccccaaatgg aagcgagaaa
      541 tgtagggcga gccttcagcc cttgtagggc ttcggcggtc aacatacctt catacggaat
      601 gcgcatggca tgaacaatat ctggctggac ttctctaatg aaagcccgca atttgaacgc
      661 ggcagcggga atggtaacag gaccaagcca ctgtcgaatt ttggtgcgca ggctcagggt
      721 ccgggaagag gcggtggagg gagcagaggt acgtttttta gcagagctaa atgccacagg
      781 ggtgaattca gtccgttcgg aggggatgcc ctccacgtcc cattcaaaag tagaagcgat
      841 aaaaacctca tccccgcgct cgataaaatg acgaatccag ttttttgaga tgggggaacg
      901 ggcatctgtg acaaatagca ggcgcataag gtgattatac attgtaaaaa tattgggcta
      961 tcgcaaccta ctttgtagta tatttaagcg catgagcgca ggacgcattc ttattgttga
     1021 agataatatg gatacctacg agctggtacg ttttattctg gagaagaacg gttttgaaac
     1081 cttcctcgct atgaatggac gagacggcgt caatgctgcc actaaacaac gcccagactt
     1141 gatcattatg gacctttcca tgcctgaaat ggatggttgg accgccatgc ggttgatcaa
     1201 aagcgaccct gccatatctt ccattcctat gatcgcccta actgctcatg ccctgcccag
     1261 tgaccgcaaa cgtgcgttcg atgcaggctg cgatgaatac atcaccaaac ccatggacct
     1321 gatcgatttg ttggataccg ttaagagttg gattcaaaaa gtataaatca ggctagggaa
     1381 aataaatggc ttatagcaat atacaaagtc tcatattaaa gagccaaatc ttcaaaaaag
     1441 gaaatacacc atgaaacaaa tttgtgtcgt cggcgtgggc tacgtcggtc tcgtgaccgc
     1501 tgcctgtttt gccgatttag gtaaccgcgt ggttgccttg gatgtcaatg aagaacgcgt
     1561 tgccaatctc aaaaagggca ttatgcccat ttacgaaccc ggcttggatg aacttgtcaa
     1621 acgcaatgta aaagcagggc gtattacgtt cacaagctcc tatacagaag cgctgaaagg
     1681 tgcagaatac gccttcattg ctgtgggtac gccttctggg tctgatgggt ctgctgattt
     1741 gcaatatgtg gcagccgctg ccacttccat tgcgaagaac atgaccgcac cattgatcct
     1801 gatcaacaag tccacggttc cgatcgggac cggtgactgg gttgccgata ttgtgaaggg
     1861 tgcccagccc aaaccgatcg acttcgccgt tgtctcttgc cccgagtttt tacgtgaagg
     1921 ctccgctatt ggcgacttca tgaatccgca ccgcaccgtg atcggttcct tggaccgtga
     1981 tgctgcaaac aaggttgctc aattgcactt acctttgcgc gcccctattg tcatcaccga
     2041 cttgcgcacg gcagagatga tcaagtacgc cagcaatgct tttctcgcca ccaaaatttc
     2101 cttcatcaat gaactcgctg acttgtgcga gcgcgttggt gcagacgtaa aagaagttgc
     2161 agcaggcatg gggtacgatg ctcgtattgg gcgtcacttt ctggatgcgg gtcttggttg
     2221 gggcggctct tgcttcccca aagatgtaga agccctcgcc ttcatggcaa aagaaaaggg
     2281 actggaaccg aagattctga acagcgtgat ggatgttaac tacgaccgcc gcaaggaagc
     2341 tgtgaaggtt gtggaaaaca tggtcggcgg cagcctcaaa ggcaagacca ttggtttgct
     2401 tggcttggct ttcaaaccca ataccgacga catgcgtgat gcgccctcca ttgatatctc
     2461 ggcagagttg attgaagcag gcgccaaggt ccgcggatat gacccggttg ccatgagtgt
     2521 ggcacgcccg atcctcccgg ctgtggaaat gtacgatgaa ccttacaaac ttgccgaagg
     2581 ctgcgatgcg ttgatgatca tcaccgaatg gaatgagttc aaacagctcg acctcgacaa
     2641 agtgaaaagc ttgctcaaat ccccggtcat ttacgatgga cgtaatatct acgaacccgg
     2701 tcgtatgaaa gacatgggct ttacctaccg cgcaatcgga cgctaatttt tcagttcatt
     2761 tgtttggtag tcgagtagtc gggtacaatc tgacggctcg actaccaatt ttttaaatac
     2821 aaaaagaaca gaattatgac caatactcct cctgtttgta actatgaagg ctccgactat
     2881 cagcaatcct tttgggatca aggcgggcgc gcttacgaag accaatctga agccattgca
     2941 ttaagacgca tgttgcccaa aagcggcaag ctgatgctgg aggtaggcgc cggtgccgga
     3001 cgcaacaccc ctcgctatgc gggctttgac cggattgtag tgatggacta ctctacgact
     3061 caactcgctc aagcgcaaga gaggctgggt cgctccgaca aatacattta cgtcgctgca
     3121 gatgtctacc gcctcccctt ccgtaatgga ctattcgatg gcgcaaccat gatccgcgtg
     3181 ctccatcaca tggcagatgc gcccaaagcg ttggggcaga ttcagggaac actacaaccc
     3241 ggggcaactt ttatccttga gtttgcgaac aagctcaacc tcaaggcgat tctgcgctat
     3301 tggctcggca agcaaacgtg gagtccgttc acgctggagc cggtggaatt cgtcaaattg
     3361 aactttgact ttcaccccaa ggctattcgt cagtggttgg gaaatcttgg tttcaatatt
     3421 gaaaagacct taaccgtctc acattttcgg cttggtttgc tcaaacgaac tattccttct
     3481 aaactgctcg ccgctgtgga tggtctcttt caaccaacag gtgcgctctt ccaactcacg
     3541 ccaagcgtat tcgtaaaagc agttttgccc ggtgcctcca gtgcagatat tccgttaaat
     3601 gtcattgacc tcttcaaatg ccccgaatgt aacagtggag ggttgcaaga gaaaacagac
     3661 catgtatttt gtccttcttg caaggcaaag tggtctgtca aagatgggat ttatgatttt
     3721 agagaaaaga taagttagaa aataaaacgc gctcaatgta gagcgcgttt tttgttattc
     3781 cttcaacaac ggcaagcgga tgaagaaggt gctgccgggg tat
//
LOCUS       DAOQJE010000249         3818 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_14825_length_3818_cov_5.762689, whole genome shotgun sequence.
ACCESSION   DAOQJE010000249 DAOQJE010000000
VERSION     DAOQJE010000249.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3818)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3818)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3818
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14825_length_3818_cov_5.762689"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1178
                     /gene="alr"
                     /locus_tag="PLT08_07820"
     CDS             <1..1178
                     /gene="alr"
                     /locus_tag="PLT08_07820"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HNE04411.1"
                     /translation="RPNLNPMWNITGTQNNMRPTYLEVNLTQLQKNIEAIRAHVGGAK
                     VMPMVKANAYGHGIEGVAPFIEPYVDMLGVALVEEGIQLRQLGIKKPILVAGGTLIEQ
                     LPLFFEHDLTLTASSPDLLLAANQLAESTRKRLTVHLKIDTGMERVGVREYEAEEFIL
                     KVFACSHLSVEGIYTHLANSELVELAGMPSHVVTGQEQLERFQEVLHLYEKHSLPVPS
                     IRHVCNSGGILNLPDGHLDMVRPGVLFYGVYPGRDIPRTIDVKPALTWKSRVSYSKVT
                     QPGRAVSYGSLWQVEGSPKRVVTIPCGYADGYFRRMTNQGRVLINGKSYSQVGRICMD
                     QFMVDAGSDDVTVGDEVILLGSGIAPEELADWAGTNEYEVMTNISARVPRVFVGGVQ"
     gene            1205..1744
                     /gene="lspA"
                     /locus_tag="PLT08_07825"
     CDS             1205..1744
                     /gene="lspA"
                     /locus_tag="PLT08_07825"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: protein motif:HMM:TIGR00077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HNE04412.1"
                     /translation="MNSKLRDYLMLFGVAGVSVALDQWTKWLVRTNIEYSGQWLPDWL
                     NWLSPYARIVHWYNSGAAFGMFQNGNLIFTILAFVVIAAILYYFPSVDSDDWTLKLAM
                     GLQLGGAVGNLIDRLIMQKVTDFISIWTFPVFNIADASISVGVAVLLLGVWLKEREEK
                     LKAAQVKTEAVPETMKEGS"
     gene            1749..2294
                     /gene="lspA"
                     /locus_tag="PLT08_07830"
     CDS             1749..2294
                     /gene="lspA"
                     /locus_tag="PLT08_07830"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: protein motif:HMM:TIGR00077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HNE04413.1"
                     /translation="MTEEISVQEKTQPRSRRFFIFFAVAVVIIALDQWTKWLVIQAVP
                     LNTSWLPEALDWLSPYARLLHIQNRGVSFGMFQGGNTVFIILTTVIVAAIVFYVSRMD
                     HPSDWMWVAAGLYLGGAVGNLIDRLTIGAVTDFISVGNFYIFNIADASINISVVLLLL
                     LSWLHERRSSTLETEPKDVSA"
     gene            2291..3217
                     /locus_tag="PLT08_07835"
     CDS             2291..3217
                     /locus_tag="PLT08_07835"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HNE04414.1"
                     /translation="MTDRIETFHYDEQNPDRLDKYLVSCLPEFSRARIQGLILDGFVS
                     INGVPAKKAGQVIEDGDDIEVRVPPPVPSGLVAEDIPLDIIFENDDLIVVNKPAGMVV
                     HPAAGHYSGTLVNAVLGYDPDLEGIGGEERPGLVHRLDKETSGLIILAKNERAHNWLQ
                     DQFRLRKVEKTYLALVDGKPPTPAGRVEAPIGRDPSHRKKMAIVSPGKGREAVSEYKT
                     LESFRHHTLLEFHPHTGRTHQIRLHCQFLGCPIVGDSVYGKRTNTATIDRHFLHAFRL
                     KIILPNEKQPRMFEAELPEELKAVLEEVKRYE"
     gene            3250..>3818
                     /locus_tag="PLT08_07840"
     CDS             3250..>3818
                     /locus_tag="PLT08_07840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntG family PLP-dependent aldolase"
                     /protein_id="HNE04415.1"
                     /translation="MNYVDLRSDTVTKPTPEMREAMAEAEVGDDVYGDDPTVNQLQVK
                     AAEMLGKEASLFVPSGTMGNLLALLVHCSRGEEVICGDKSHIYVNEAGGMAALGGIYP
                     HPVPNQKDGTLRLEDIRASIQPDDSHRTITRLVCIENTQNMCGGVVLSVEYTKQVGQL
                     AKEYGLKFHIDGARIFNAAAALNVDIKELV"
BASE COUNT          927 a          805 c         1058 g         1028 t
ORIGIN      
        1 gccgcccgaa tctgaacccg atgtggaata tcactggtac tcaaaataat atgcgtccca
       61 cttatcttga agtcaatctc acccaactgc aaaaaaatat cgaagccatc cgcgctcatg
      121 tgggtggcgc gaaggtcatg ccgatggtca aagccaatgc ctatggtcac ggcatcgaag
      181 gcgttgcgcc gttcatcgaa ccgtatgtcg acatgctcgg tgtcgctttg gtggaggagg
      241 gcatccaact tcgtcaactc gggatcaaga aaccgatcct cgttgccggt gggacgttaa
      301 tcgagcaact gcctttattc tttgaacatg acttaaccct aaccgcttca tcccctgatt
      361 tgttgctggc tgctaaccag ttagccgaat ctacccgaaa gcgactcacc gtccacctca
      421 agatcgatac cggcatggag cgggtcggcg tccgcgaata cgaagcggaa gagttcattc
      481 tgaaagtgtt tgcgtgtagt catttaagcg tggaggggat ttatacacat ctcgcaaact
      541 cagagctggt tgagctggct gggatgcctt cgcatgttgt tacgggtcaa gaacagcttg
      601 agcgttttca agaagtactt cacttatatg aaaaacactc tttgcctgtg ccatcgattc
      661 gacacgtctg caattctggt gggattctga atctacctga tgggcattta gacatggtgc
      721 gtcctggcgt cttgttttat ggagtttacc cagggcgcga tattccacgg acaattgacg
      781 tgaaaccagc actcacttgg aaatcacgag tctcgtatag caaggtcaca cagccggggc
      841 gtgctgtcag ctatgggtcg ttgtggcagg tcgaagggag tccgaagcgc gtggtcacca
      901 tcccatgcgg gtatgccgat ggctacttcc gccgtatgac caatcaagga cgagtcctca
      961 tcaacggcaa gtcgtattcg caagtaggtc gtatttgcat ggatcaattc atggtcgatg
     1021 ctgggagcga tgatgtaacg gtgggggatg aggtgatatt gttgggcagc ggcattgcgc
     1081 cagaagagtt ggcagattgg gcaggcacca acgaatatga ggtgatgacc aacatcagcg
     1141 cccgcgtgcc gagggtattt gtgggtgggg tacaataggc gcacttttcg ttttgaggtg
     1201 agagttgaat tccaagttaa gagattattt aatgctgttc ggcgtggctg gcgtttcggt
     1261 tgcactcgat cagtggacaa agtggttggt acgtacaaat attgaatata gtgggcagtg
     1321 gttacctgat tggttaaatt ggttaagccc ttatgcacgt attgtgcatt ggtataacag
     1381 cggcgctgcg tttggcatgt ttcaaaatgg aaacttgatt ttcactattt tggcatttgt
     1441 tgtgattgct gccattcttt attactttcc cagtgttgac tcagatgatt ggacgttgaa
     1501 attggcgatg gggttgcaat tgggcggcgc ggttggaaat ttgatcgacc gtttgattat
     1561 gcaaaaggtg acagatttta tttcgatctg gaccttccca gtttttaata ttgctgatgc
     1621 atccatttcg gtgggggtag cggttttatt attgggggta tggcttaagg agcgagaaga
     1681 gaaactaaag gcggctcagg taaaaacgga agccgtgcca gaaacaatga aagaaggttc
     1741 gtagtttgat gactgaagaa atcagtgtac aagaaaaaac gcagcctcgt agcaggcgtt
     1801 tttttatctt ttttgccgtg gcagtcgtga tcattgccct cgaccagtgg acaaagtggc
     1861 tggtgattca agctgtccct ctcaacacat cttggttgcc tgaagcgctg gattggcttt
     1921 cgccatatgc ccgtttgctc catattcaga atcgcggtgt ttcgtttggg atgttccagg
     1981 gcgggaatac ggtgtttatc atcttgacga ctgtgatcgt ggcggcgatt gtcttttatg
     2041 tgtctcgtat ggatcacccc agtgattgga tgtgggtggc ggctggcttg tatttgggcg
     2101 gtgccgttgg aaacttgatc gaccgcctca cgattggagc cgtaacggat ttcatttccg
     2161 tgggtaactt ttacatcttc aatattgcag atgctagtat caacataagt gtcgttttgt
     2221 tattgctttt atcttggttg catgaacgaa gatcgtcaac gcttgaaact gagcccaagg
     2281 acgtgagcgc atgaccgacc gaattgaaac attccattat gacgaacaga atccagaccg
     2341 gctggataaa tatcttgttt cctgtctgcc tgagttttca cgtgcccgta ttcagggctt
     2401 gattttggat ggatttgtat cgatcaatgg tgtgcctgct aaaaaagcag ggcaagtaat
     2461 tgaagatggc gacgatatcg aagtgcgagt tccgccgcca gtgccaagtg gattggtcgc
     2521 agaagatatt ccgcttgata ttatttttga aaacgatgac ctgattgtgg tgaacaaacc
     2581 tgccggtatg gtcgtccacc cggcggcagg gcattactct ggaaccttgg tcaatgcagt
     2641 attagggtat gacccagacc ttgaaggtat tggtggcgaa gaacgccccg gtttagtaca
     2701 tcgtttggat aaagagactt caggtttgat catccttgcc aagaatgaac gcgctcacaa
     2761 ctggctgcaa gaccagttcc gcttgcgaaa agtggaaaag acctatcttg cgcttgtaga
     2821 tggcaagcca ccaacaccgg ctgggcgtgt agaagcgccg attgggcgtg acccaagtca
     2881 tcgcaaaaag atggcgatag tttcaccggg taaaggacgt gaagcggtca gtgaatacaa
     2941 gacgctagag tcttttaggc atcatactct gttggagttt catccacata cgggtagaac
     3001 acatcagatc cgtttgcatt gtcaattctt gggttgcccg atcgtgggag actcagttta
     3061 tgggaagcga actaacactg ctaccattga ccggcatttc ttgcatgcgt ttcggttgaa
     3121 gattatcctt ccgaacgaaa aacagccccg catgtttgaa gcggaattac cagaggaatt
     3181 gaaagctgtc ttggaagaag taaagagata tgagtaagta gcaccctact tgaataaacg
     3241 gagatttcca tgaactatgt agatttacga tctgatactg taaccaaacc gacgcccgaa
     3301 atgcgtgagg caatggcaga agcggaggtg ggggatgatg tttacgggga tgacccaacg
     3361 gtgaatcaat tgcaggtgaa ggcggcagag atgcttggta aggaggcgtc tcttttcgtc
     3421 ccttcgggca cgatgggcaa tttgttggca ttgcttgtac attgttcgcg cggcgaagaa
     3481 gtgatctgcg gcgacaagtc gcatatttat gtcaacgaag ctggtgggat ggctgcgttg
     3541 ggcgggattt atcctcatcc cgtgccgaat caaaaggatg gaacacttcg gctcgaggac
     3601 atccgcgcct cgattcaacc cgatgactca catcgcacca ttacgcgttt ggtttgcatt
     3661 gagaacacgc agaacatgtg cgggggtgtt gtcctttctg tggagtacac aaagcaggtt
     3721 ggtcaactcg caaaagagta tggattgaaa ttccatattg atggtgcgcg tattttcaat
     3781 gcggcggctg ctttgaatgt ggatatcaaa gaacttgt
//
LOCUS       DAOQJE010000250         3814 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_14846_length_3814_cov_7.201383, whole genome shotgun sequence.
ACCESSION   DAOQJE010000250 DAOQJE010000000
VERSION     DAOQJE010000250.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3814)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3814)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3814
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14846_length_3814_cov_7.201383"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..455
                     /locus_tag="PLT08_07845"
     CDS             <1..455
                     /locus_tag="PLT08_07845"
                     /inference="COORDINATES: protein motif:HMM:NF013702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HNE04416.1"
                     /translation="DFLLFLGIVFHLGISVRYMGKAELFNNPFGWFFYWCGGVPVDRK
                     KSVGLVDQMVEVCNQSDKFILVIAPEGTRYQVNEWKRGFYHIAKSANIPIVTARVDGI
                     QKIAHVGQIFQPGDDIEADMKAIQESFIGMTGINPRLKTKKKYITLAN"
     gene            456..911
                     /locus_tag="PLT08_07850"
     CDS             456..911
                     /locus_tag="PLT08_07850"
                     /inference="COORDINATES: protein motif:HMM:TIGR00022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhcH/YjgK/YiaL family protein"
                     /protein_id="HNE04417.1"
                     /translation="MIIDRIENIALYVSLHPRFKQAFDYLSQIDIHAISVGRHDVDGD
                     AIYALVQEYTTKQTGEGKWEAHRRYIDLQYVVQGAEGMGYANIHHLRQGEYVPEKDFL
                     PLFGEGDQVPLRSGNFVLLFPEDAHMPSMALGEPAPARKIVLKIAVEAG"
     gene            complement(938..2605)
                     /locus_tag="PLT08_07855"
     CDS             complement(938..2605)
                     /locus_tag="PLT08_07855"
                     /inference="COORDINATES: protein motif:HMM:NF017011.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE04418.1"
                     /translation="MGLEKFSSIFYVLLSDTICTTMKEFFTQRIKRGLVRRLTLLKVA
                     SVAREVARKEPQPNGAPIVFFKASTGIDDLSWNSGFHLLTTWALRLQGIPVAYFACNS
                     GLSKCVLGTNRDNPHKEMPCKSCVMQSKALYTGVEGHKSKVGWFDFQRDPELVSRISS
                     LSVSELSTFHFQDLPLGELCLPALRWILRIHHLNDDENTRYLLREYILSAWNVAQKFS
                     KFLDETSPRLVVVFNGQFYPEATARYLAKKRGLRVITHEVGLEPATAYFTEGEATAYP
                     IHIPDEFELSEAQNARLDAYLAKRFKGDFTMAGVKFWADMKGLDEAFLQKAAQFKQIV
                     PIFTNVIFDTSQPHSNTVFEDMFAWLDTTLEVIKEHPETLFIIRAHPDELRLRKASRE
                     TVGGWVESTRVTSLPNVVFVPPNETLSSYELIAKSKFVMIYNSTIGLEASIMGAAVLC
                     AGKARFTQYPTVFFPQTVEDVRRKMKEFLTAETIKVPVEFKRNARRFLYYQLYRTSLP
                     LGQFLEPSVRVTQTKLKTFDLAELQKSKSMQAILDGLLNNGDFLLKE"
     gene            complement(2613..3467)
                     /locus_tag="PLT08_07860"
     CDS             complement(2613..3467)
                     /locus_tag="PLT08_07860"
                     /inference="COORDINATES: protein motif:HMM:NF012888.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008146 - sulfotransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase domain-containing protein"
                     /protein_id="HNE04419.1"
                     /translation="MGIKQKLRRALRPYAKTAQAIWHWKRFSFNDAPPIFANSKPKSG
                     SHLLLQVMNGFTQIMPYKYVDADPVRTITYKGRRKTNEEILADLKHTPKGVIGWGYVD
                     ATPENASFLTSAGRVNYFIYRDPRDMLVSQVFFATDMHEEHGMHDYYNSLPDFGARFN
                     VAITGIDRDGLKMVSVKQRYEGVFQWLEQKNTMCLRFEDLINNRDATLLKMLDEVEKT
                     GYKIPTPREKALPILVEAIQPKKSHTFRSGKTGGWAQHFTAEHKKLFKDVAGDLLVRL
                     GYEKNNDW"
     gene            complement(3515..>3814)
                     /locus_tag="PLT08_07865"
     CDS             complement(3515..>3814)
                     /locus_tag="PLT08_07865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016051 - carbohydrate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate synthase family protein"
                     /protein_id="HNE04420.1"
                     /translation="MIETLRKEFPNNPIGYSGHETGLVPSAVAVALGACSVERHITLD
                     RAMWGSDHAASVEPGGFEKLVKYIRVTEAGLGDGVKRVYESEKAPMKKLRRVVNE"
BASE COUNT          989 a          884 c          945 g          996 t
ORIGIN      
        1 gggatttttt gctgttcctt ggcattgtct ttcaccttgg cattagtgtg aggtatatgg
       61 gcaaagccga gttgttcaac aatccttttg gatggttctt ttattggtgt ggcggtgtgc
      121 ctgtggacag aaaaaagtct gttgggctgg tcgatcaaat ggtggaagtt tgcaaccaat
      181 ctgataaatt tattttggtc atcgcccccg aagggacgcg ttatcaggtc aacgaatgga
      241 agcgtgggtt ttatcatatt gccaagagcg ccaacattcc cattgtgacg gcaagggtgg
      301 atggcattca aaagattgca catgtgggtc agatatttca gcctggtgat gatattgaag
      361 cagatatgaa agcgattcag gaatctttca ttggcatgac gggcatcaat ccacgcttaa
      421 agacaaagaa aaaatatatt acattggcaa attaaatgat catagaccgc attgaaaaca
      481 ttgccctata tgtttcgctt catccgcgtt tcaagcaggc atttgattac ttgagccaga
      541 tcgatattca tgcgatatcc gttgggcggc atgatgtaga tggtgacgcc atctacgctt
      601 tggttcagga atacacgacc aaacaaacag gggaaggaaa atgggaagct catcgccgct
      661 atatcgactt gcaatatgtg gtgcaaggcg cagaaggcat gggctacgct aatatccatc
      721 atctacgcca aggggaatat gtacctgaga aggactttct gcctttattt ggcgaaggtg
      781 atcaagtgcc gttaagaagt ggtaactttg tattgctatt ccccgaagat gctcatatgc
      841 ccagtatggc attgggtgag cctgcacctg ctagaaagat tgttttgaaa attgcagtag
      901 aggcggggta ataccgcttc gtggcacaaa ccccgcccta ctcttttagt aagaaatccc
      961 cgttattcaa caagccatct aagattgcct gcatagactt agatttctgt agctcagcca
     1021 aatcaaacgt ctttaacttc gtttgagtca cacggaccga cggctcaaga aattgaccta
     1081 agggcaaaga agttcgatat aactgataat acaagaagcg acgtgcgttg cgtttgaatt
     1141 cgacagggac tttgatcgtc tcagccgtca aaaactcttt catctttctt cgtacatctt
     1201 ccactgtttg cgggaagaat acggtgggat attgagtaaa acgcgcctta ccagcacaca
     1261 agaccgcagc ccccataatg gacgcttcca agccaatggt cgagttatag atcatgacaa
     1321 atttagattt cgcgattaat tcataggaac ttagcgtttc gtttggcgga acaaaaacaa
     1381 cattcggcaa actggtgact cgggtagatt ccacccatcc gcccactgtt tcacggctgg
     1441 ctttgcgaag acgtagctca tcggggtgag cgcggataat gaacaaggtc tctgggtgtt
     1501 ctttgatgac ctccagagtc gtatctaacc aagcaaacat atcttcaaag accgtgttgg
     1561 aatgtggctg actggtatca aagataacat tagtaaagat gggcacgatt tgtttgaact
     1621 gagccgcttt ttgaaggaag gcttcgtcca gtcctttcat atcagcccaa aatttgacgc
     1681 ccgccatcgt aaagtcacct ttgaagcgtt tggcaagata agcatcgagc cgtgcatttt
     1741 gcgcctcgct tagctcaaat tcatcgggga tatgaatcgg gtaggctgtc gcttcgcctt
     1801 cggtgaaata ggcagtagca ggctccaagc ccacctcatg ggtgatgacg cgcagtccgc
     1861 gttttttggc gagataccgt gccgtcgctt cggggtagaa ctgtccattg aagaccacca
     1921 ccaaacgtgg acttgtctca tcgagaaact tcgagaactt ttgcgccaca ttccatgcgg
     1981 acaaaatgta ttcacgcaaa agataacggg tgttttcgtc gtcattcaag tgatgaatac
     2041 gaaggatcca acgcagggcg ggcaagcaca attcaccaag aggaaggtct tgaaagtgga
     2101 aagtggaaag ttcagagaca gaaagtgatg agatacgaga aacgagttca ggatcacgct
     2161 gaaaatcaaa ccaaccgact tttgacttgt gaccttcgac tcctgtataa agcgccttgc
     2221 tctgcatcac acaagattta caaggcattt ctttatgggg attatcgcgg ttcgtaccca
     2281 atacgcattt gctcagccct gagttacaag caaagtatgc cacagggatg ccctgcaagc
     2341 gcaaggccca agtggtcaac aaatggaacc cactgttcca tgagaggtca tcaatgccag
     2401 tggaggcttt gaagaataca attggtgcgc catttggttg cggctcttta cgagccacct
     2461 ctcgcgcaac ggatgcaact ttgaggaggg tgagtctgcg gacgagtccg cgtttgatgc
     2521 gttgagtgaa gaattctttc atagtcgtgc aaattgtatc agagagcaga acataaaaaa
     2581 tggaactgaa tttctccagt cccatgcata tactaccagt cgttgttctt ttcgtaccct
     2641 aacctgacca gcaaatctcc tgctacatcc ttgaaaagct tcttatgttc tgcggtgaag
     2701 tgctgagccc aaccgccggt tttgcccgag cggaaggtat gacttttctt tggctgaatg
     2761 gcttccacca aaataggtaa tgctttttca cgcggagtgg ggatcttgta gccggtcttt
     2821 tccacttcat cgagcatttt taacaaggtc gcatcgcggt tgttgatgag gtcttcaaaa
     2881 cgaagacaca tcgtattttt ttgctccaac cactggaaga cgccttcata cctctgcttc
     2941 acgctaacca tcttcagccc gtcacggtca atgccggtga tggcaacatt gaaacgtgcg
     3001 ccaaaatcag gcagggaatt gtagtaatcg tgcatgccat gttcttcgtg catatcggtc
     3061 gcgaagaaca cttgtgagac gagcatatct ctcgggtcgc gataaataaa atagttcacg
     3121 cgcccggctg aggtcaagaa agaggcgttc tctggggtgg catccacata gccccaaccg
     3181 atcactccct ttggggtgtg cttcagatcc gcaagaattt cttcgttcgt ttttcttctg
     3241 cctttgtagg tgatcgttcg cacggggtcg gcatccacat acttgtaggg catgatctgc
     3301 gtgaagccat tcatcacctg caaaagcaaa tgcgaaccgc tcttgggctt tgagttagca
     3361 aagatgggcg gtgcatcgtt aaacgaaaac cgcttccaat gccagatggc ttgggcggtc
     3421 tttgcatacg ggcggagtgc gcggcggagt ttttgtttaa ttcccataat ttaataaata
     3481 taggggcgag gtaacctcgc ccctacaaaa ttctttattc gtttaccaca cggcgaagtt
     3541 tcttcattgg agctttttca gattcataca cacgcttcac gccatcacca agcccggctt
     3601 cggtgacacg gatgtatttg actagcttct caaaaccacc gggttccaca gaggcggcat
     3661 ggtcactgcc ccacatggcg cggtctaggg tgatgtggcg ctccacggag caggcgccca
     3721 gcgccaccgc cacggcagaa ggcacaagcc cggtttcatg accagaatac ccgatcgggt
     3781 tattggggaa ttccttgcgc agggtttcga tcat
//
LOCUS       DAOQJE010000251         3814 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_14847_length_3814_cov_6.140463, whole genome shotgun sequence.
ACCESSION   DAOQJE010000251 DAOQJE010000000
VERSION     DAOQJE010000251.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3814)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3814)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3814
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14847_length_3814_cov_6.140463"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..214)
                     /locus_tag="PLT08_07870"
     CDS             complement(<1..214)
                     /locus_tag="PLT08_07870"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HNE04421.1"
                     /translation="MRHHRTSILIADDHEVVRSGIRSYLEKISDFQVVGEASSGEETL
                     SLVSELIPDIVLLDLILPGMDGIETTR"
     gene            complement(216..>3814)
                     /locus_tag="PLT08_07875"
     CDS             complement(216..>3814)
                     /locus_tag="PLT08_07875"
                     /inference="COORDINATES: protein motif:HMM:NF024583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="two-component regulator propeller
                     domain-containing protein"
                     /protein_id="HNE04422.1"
                     /translation="GKNIRFEQISLEEGLSQSVVNVILQDQKGFLWIGTDDGLNRYDG
                     YNFKIFKPDTTNPFSLSDRSITDIVEDKDGNLWIATRMGGLNRYDPISGKFTQYMHSK
                     NDATSIISNQVTSLFVDKTGLWVGTNNGLDFLDFSSNQFIHYPLATGKLVPESKSISV
                     VYKDSANRLWIGTSDAGLGLYDLKDQSFKAFKNNEYNNQSLSHNRILAIKQDLAGNIW
                     VGTSNGLNLYNEEEGDFTRFKRSKDDPNSIAGNIIYSLFTDQYGSLWVGTNVGLDRYS
                     PQEGNFIHHQYQPNVPNSLSNNEVLYIYEDDSGVLWVGTYGGGLNKYNRQQDRYAYFR
                     NNPDDPASLSSNIIFAIQPAEFGKVWVGTLDSGLNLFEPYTETFTRFVHDPNTPNSLI
                     NNSIFSLYRDKAGLIWVGTGNGLDRYNRGSKSFTHYSPNESDFTRTDERFNVLAILED
                     TQKNFWVGSSMGLYLFDRKAGTLTRHNSELGTTRVNALLEDKDGNLWVGTPEDGLRRL
                     NTQTGEISYFRYDSTEKTSLSNNSVLCIYQDPQGTIWIGTHGGGLSRFNPETETFTHF
                     TEQEGLSNNVVYGILEDESGKLWLSTNFGLARFNPINQTFRIFTASDGLQSNEFNQNS
                     YAKDENGTMYFGGINGLNVFTPQEITDNQYTPKLSLTSITVDGTPFTNKDGRTTEYLD
                     TITLTWPQDSFEFEFAAFAFEQPLNNRYAYKLEGFDTDWISTDKQRNGRYTNLPGGTY
                     TLRLRGSNNDGVWNDQGASIQVVVVPPLWETWWFRGILVVAFMFSVVGGLRWRVKSVE
                     NKNLELERLVKIRTADLEKRTGEIEALYQADERILRSVTLNQIFQALVDVSISILKAD
                     RSMVLTWNDEKRKITPRISLGFRPETLAVMNFDYDEGLIGISMKTGHPMIASEENLSS
                     LREDTRKAIEVEGIRSFAHFPIVVDEKVIAIFNVAYLRSNALTKDSIRLFTALVNRAA
                     MAIANMELFEQTRDLAVMEERNRLARDLHDSAKQKAFAALAQLGTVNGMAKAVPAGPV
                     VPIGAEQHLSEAENLVYEVIQELNFLIQEIYPIALQEKGLPTVLREYIFEWENRNDIV
                     ANLMIQNERRLQLETEQAIYRVIQEALANVSRHSQAKRVDISLVYNPDLLQVAMADDG
                     CGFNVNQKATGLGFRSMRERIISVRGTFQIQSAPGQGTRIIIQIPLKANSGG"
BASE COUNT          994 a          854 c          861 g         1105 t
ORIGIN      
        1 ggcgtgtagt ttcgatgcca tccataccgg gaaggatcaa gtcaagaaga acaatgtcgg
       61 ggattaactc agagaccaaa ctcagagtct cttctccgga tgaagcttca cctacaactt
      121 gaaaatctga aattttttca aggtaagaac ggatgccact tcgcactact tcgtggtcat
      181 ctgcgatcaa aatacttgtg cggtgatgtc tcatgttaac ctcctgaatt ggctttgaga
      241 gggatttgaa taataatacg cgttccctgc ccgggcgcac tttggatttg gaaggtgccg
      301 cgtacactga taatacgttc tcgcatggaa cggaaaccca agccggtcgc tttttggttg
      361 acattgaacc cgcaaccatc gtctgccatg gcaacttgaa gaaggtctgg gttgtatacc
      421 aatgaaatat ctacacgttt tgcttggcta tggcgggaaa catttgccaa cgcctcttga
      481 atcacgcggt aaattgcttg ctctgtctca agctgtagcc tccgctcgtt ctgaatcatg
      541 agattggcga caatatcgtt tctattttcc cattcaaaaa tgtactcacg cagaacagtg
      601 ggcaatcctt tttcttgtaa agcgattggg taaatctctt ggatcaagaa gttcaattcc
      661 tgaatcactt cgtagaccag attttcggct tcactcaaat gttgctctgc tcctataggg
      721 acgacaggac ctgctggaac agcttttgcc atgccgttta cggttcctaa ttgtgcgagt
      781 gcagcaaagg ctttttgttt ggcagagtca tgcaggtcac gcgcgaggcg gtttctttct
      841 tccatcactg ctaggtcgcg agtctgctca aatagctcca tgtttgcgat cgccatggct
      901 gctcggttca ccagcgcggt aaagagacga atggagtctt tggttaaagc gttagagcgc
      961 agataagcaa cattgaaaat tgcgataacc ttttcatcga ctacgatggg gaagtgggcg
     1021 aaagaccgaa tgccttcaac ttcaatggct ttgcgagtgt cttcacgcag cgacgataaa
     1081 ttttcttcag aagcgatcat gggatgacca gttttcatag aaataccgat caaaccttca
     1141 tcgtaatcaa agttcatcac agcgagagtt tcagggcgga aacccagact aatgcgtgga
     1201 gtgatcttcc gcttttcatc attccaggta agcaccatgc tacgatctgc tttgagaatg
     1261 gaaatggaca catccaccag agcctgaaat atctggttga gtgtgacgct gcgtaaaatc
     1321 cgctcatcag cttgatacaa agcttcgatc tcgccagttc gtttttcaag gtcagctgta
     1381 cgaattttga ccagccgttc cagttccaga tttttatttt ccacactttt tactcgccag
     1441 cgtaaaccac ctactacgct gaacatgaaa gcaacaacta gaatgccacg gaaccaccaa
     1501 gtttcccaga gaggtggtac cactaccact tgaatcgatg caccttggtc gttccaaact
     1561 ccgtcattgt tggaaccgcg caggcgtaaa gtataggttc cgccagggag gttggtgtac
     1621 ctgccgtttc tttgtttatc agtgctgatc caatcggtat caaacccttc taacttgtag
     1681 gcataacgat tattgagagg ttgttcaaaa gcaaacgctg caaactcaaa ttcgaaggag
     1741 tcttggggcc aggtgagggt gatggtatca agatactcgg tggtgcgacc atccttattt
     1801 gtgaaaggtg tgccatctac agtaatagag gttaatgaga gtttcggcgt atattggtta
     1861 tctgtaattt cttgaggcgt aaagacatta agcccattga tgccaccaaa gtacatggtg
     1921 ccgttttcat cttttgcata agagttctgg ttgaattcgt tgctttgtaa accatcgctt
     1981 gccgtaaaaa tacggaaagt ttggttgatc ggattaaacc gggcaagccc aaaatttgtg
     2041 ctcaaccaaa gctttccgga ttcatcttcc aaaattccat atacaacgtt gttcgataga
     2101 ccttcctgtt cagtaaaatg ggtgaaggtc tctgtctctg ggttaaaacg gctcaaaccg
     2161 ccgccatgtg tcccaatcca aatagtgcct tgtggatctt ggtagataca taacacgcta
     2221 ttgttgctca acgaagtctt ttctgttgag tcatatcgga aataagatat ttcaccggtt
     2281 tgggtattaa gacggcgtaa cccatcttct ggtgtaccta cccatagatt gccatcttta
     2341 tcttctaaaa gcgcattgac tcgtgtggtg ccaagctcgc tattgtgcct ggtaagagtt
     2401 ccagctttcc tatcgaaaag atagagcccc atgctggacc ccacccaaaa gtttttctgc
     2461 gtgtcttcca gaatggctaa aacattaaac cgttcatcgg ttcttgtgaa atcactctcg
     2521 ttgggagagt agtgcgtaaa gcttttcgag ccgcgattgt agcggtctag cccattccct
     2581 gtgccaaccc agatcaagcc ggctttgtct ctatagagag agaagatgct gttgttgata
     2641 aggctgttgg gtgtgttcgg gtcgtggaca aaacgtgtaa aggtctctgt gtacggttca
     2701 aatagattta acccgctatc gagagtgccc acccaaacct tcccaaactc tgcgggttga
     2761 attgcaaaaa tgatgttgct gcttaagctg gcagggtcat cgggattatt gcggaagtaa
     2821 gcataccgat cttgttggcg gttgtatttg tttaatccgc cgccgtaggt gccaacccat
     2881 aacactccgc tatcatcttc gtagatatat agcacttcgt tattgctcaa actgttcggc
     2941 acgtttggct gatattggtg gtgaataaag ttaccttctt gtggagaata acggtctaac
     3001 cctacatttg tcccaaccca taaacttccg tattggtcag taaataagga gtaaatgata
     3061 ttgcctgcaa tactatttgg gtcatctttc gatctcttaa aacgagtgaa atcaccttct
     3121 tcttcgttgt agagattcaa tccattacta gtgccaaccc agatattgcc agcgaggtct
     3181 tgtttgatcg caagaatgcg attgtgactc aagctctgat tgttgtactc attattttta
     3241 aacgccttga aagactggtc cttcaaatca taaagtccta agccagcgtc tgatgtgcca
     3301 atccaaagcc ggttggcaga gtctttatat acaacagaaa tggatttact ttcagggacg
     3361 agcttgcccg tagcgagcgg atagtgaatg aattggttgg atgagaaatc aagaaaatcg
     3421 agcccattgt ttgtgccaac ccaaagcccg gttttatcta caaaaagtga tgtgacttga
     3481 ttgctgataa tactggtcgc atcgttttta ctatgcatgt attgagtgaa cttacccgag
     3541 atggggtcgt aacgatttaa tccccccata cgagtggcga tccacaaatt tccatctttg
     3601 tcttcaacaa tatcagtgat cgaacggtca cttaaactga atggattggt agtatcaggt
     3661 ttgaatatct tgaagttgta gccatcataa cggttcaacc cgtcatcggt gccgatccat
     3721 aaaaaacctt tttggtcttg caagattaca tttacaacac tttgcgaaag accctcttca
     3781 agactaatct gttcaaagcg gatgttcttt ccgt
//
LOCUS       DAOQJE010000252         3799 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_14935_length_3799_cov_5.451923, whole genome shotgun sequence.
ACCESSION   DAOQJE010000252 DAOQJE010000000
VERSION     DAOQJE010000252.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3799)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3799)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3799
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14935_length_3799_cov_5.451923"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(14..670)
                     /locus_tag="PLT08_07880"
     CDS             complement(14..670)
                     /locus_tag="PLT08_07880"
                     /inference="COORDINATES: protein motif:HMM:NF012698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HNE04423.1"
                     /translation="MKTTTLLVIEAKHAEVPSFAADLQKKGFDVHIAQNGSKAVALLK
                     EVSPSLVVVNAASMRSTGLRICQSLREKDSKLPIILILDEEKEVSKDAADAVLILPFT
                     VQKLSNRIKPLLPGDGKNVLHVGPIRLDLEKRRVRCLGKSTKLTPRLVTLLQLLMEKH
                     GEVVEREPLFKKAWETNYTGDTRTLDVHISWLRRAIELDPDNPKFLKTVRGVGYRLDI
                     "
     gene            complement(842..1918)
                     /gene="rlmN"
                     /locus_tag="PLT08_07885"
     CDS             complement(842..1918)
                     /gene="rlmN"
                     /locus_tag="PLT08_07885"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: protein motif:HMM:TIGR00048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016433 - rRNA (adenine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HNE04424.1"
                     /translation="MLIYDLNLPALESVLKEWNEPAYRAKQIWQGLYQHLYSSPEQFS
                     NLPNSLRSKMAEHFTFNPFTIKTYLDSSDKSTRKTLFQLPDGNLIESVLMRYGDPADE
                     HSTSSEKASKQRRTLCISTQAGCAMGCVFCATGQLGFKRNLSSGEIVAQVMYYAQMLK
                     GQEEVVSNVVFMGMGEPFHNYDNVMSAIDRLNDADGYKFGARRLTISTVGLVPQIRRF
                     ADEQRQVNLAISLHAADDDERLAIMPVNKRYKIDDVIEACKYYIEKTHRRVTFEWALI
                     NGVNDTPEVAQKLASRLKGLLCHVNAIPLNPTQGYQGEATSRQRAQVFRETLEQAGIP
                     CTIRMRRGIDINAGCGQLAGAESK"
     gene            2123..3154
                     /locus_tag="PLT08_07890"
     CDS             2123..3154
                     /locus_tag="PLT08_07890"
                     /inference="COORDINATES: protein motif:HMM:NF027484.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NBR1-Ig-like domain-containing protein"
                     /protein_id="HNE04425.1"
                     /translation="MLKPMKPKQTFPNNTILWILVGVLVIGGIIAIVSAFSGGGKNAE
                     DEVNAVYTNAAATLAIQQMTLQAASPSVTPTVATPTGTVTFTPQPLATLPTSQGISTS
                     SSSSSGGSGAVGCNNSVFVSDVTVPDDTVMTPGQSFTKTWKLQNTGSCPWTTSFKVSF
                     VSGNAMSGTTTALTTTVQAGQSGDISVAMKAPATTGDAIGYWILTNDSGQNFGSSFYV
                     KIKVGGTSTTGTVTATATATATTAAVTAPTAASNVAITLTCTLGGTNGTQKEHAGTLT
                     WTDNSNNETGFNIYINSVFAATVAANTTSFGVPSGTFYDTGVTSTYAVEAVNGSTKAA
                     QATVTVSCP"
     gene            complement(3211..>3799)
                     /locus_tag="PLT08_07895"
     CDS             complement(3211..>3799)
                     /locus_tag="PLT08_07895"
                     /inference="COORDINATES: protein motif:HMM:NF024639.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="HAD hydrolase-like protein"
                     /protein_id="HNE04426.1"
                     /translation="DVEAEQVVTSALGIAFMMAQRHPRGTKIFMIGENGIRVALEEKG
                     FEVVGVEDAHQAEAVVMGIDRGISFQKVAEATLLVRAGRPFYTTNTDRTFPTPRGEIP
                     GSGSWLSVVTSATGVEPMVAGKPFPFLMELSLERLGTSKEETLVVGDRLETDIAAGQA
                     VGCPTAFVLSGVSKLEEAKEWNPSIIADSLAKLVE"
BASE COUNT         1003 a          908 c          851 g         1037 t
ORIGIN      
        1 aaagcaattt cagctaaata tctaagcgat acccaacacc gcgcacggtc ttgagaaatt
       61 ttgggttatc ggggtccagc tcaatggcgc gtcgcaacca tgagatatgt acatcaaggg
      121 tgcgggtatc accggtgtaa ttggtttccc atgctttttt gaagagcggt tcacgttcca
      181 ccacttctcc atgtttttcc atcaacaatt gaagcaaggt gaccaaacgc ggggtgagtt
      241 ttgtgctctt acctaaacag cgcacacgtc gtttttcaag gtctaaccga ataggaccca
      301 catgcaaaac gttttttccg tcaccgggca ataaaggctt gatgcgattg gaaagttttt
      361 gaactgtgaa cggcaaaatc aaaactgcat cggctgcatc tttgctaact tctttttctt
      421 catccagaat aagaataatg gggagctttg aatctttttc gcgcagcgac tgacagatgc
      481 gtaaaccagt acttcgcatg gatgccgcat tgacaacaac caagcttggg ctgacttctt
      541 tcaacaacgc aacagctttg ctgccattct gtgcgatatg tacatcaaat cccttcttct
      601 gcaagtctgc tgcgaaagaa gggacttctg cgtgtttggc ttcaatgacc aatagggttg
      661 tggtcttcat aagaaatttt gataatgcct ttttgcaaat tattgctgca acgagctttt
      721 aagcttgtta agattgattc attatacacc atatcttaac aaagggtctc aaacctgagt
      781 actatttgca tcttaatttc attgacatat tttttaagtt gtggaatcaa atcagaaaat
      841 tttattttga ctcagcgcct gcaagttgac cacagcccgc attgatatca ataccgcggc
      901 gcatgcggat cgtgcatgga attccggctt gttccaatgt ttctctgaat acttgcgcac
      961 gctgacggct cgtcgcttcg ccttgatagc cttgtgtggg gtttaatgga atggcattca
     1021 catgacaaag caagcctttg agtctggagg cgagtttttg cgccacttcc ggcgtatcgt
     1081 tgactccatt aatgagcgcc cattcaaagg tcacgcgacg atgcgttttt tcaatatagt
     1141 atttgcaggc ttcgataaca tcatcaatct tataacgttt gttgaccggc ataatcgcga
     1201 gacgctcatc gtcatcggcg gcatgaaggg aaatggctaa gttgacttgg cgctgttcat
     1261 ctgcaaagcg ccgaatttgt ggcacaagcc caaccgtaga aatggtcaag cggcgtgccc
     1321 cgaatttgta tccatcggca tcgttcaaac gatcaatggc agacatcaca ttatcgtagt
     1381 tgtgaaaagg ctcgcccatg cccataaaaa cgacgttgct tacaacttcc tcttgtcctt
     1441 tgagcatttg cgcgtaatac ataacctgcg ccacgatctc accgctggaa agattgcgct
     1501 tgaacccgag ctgacccgtg gcgcagaaca cacatcccat agcgcagcca gcctgcgtgg
     1561 agatgcaaag cgtgcgcctt tgctttgagg ctttttcaga agaagtggaa tgctcatcgg
     1621 cggggtcgcc ataacgcatc aagacagact cgatcaaatt cccatcaggc agttgaaata
     1681 acgttttacg ggtagattta tcagatgagt ctaaataggt tttaattgtg aatggattaa
     1741 aggtaaaatg ctcagccatt ttgctgcgca gggaattcgg cagattgctg aattgttctg
     1801 gtgaagaata aagatgttga tacagtccct gccagatttg cttggcgcgg tatgcaggtt
     1861 cattccattc ttttaagaca gattcaagag cgggaaggtt aagatcgtaa attaacataa
     1921 atggtttcct attttacacc agcaaatgga aatcccattt ttcgtaggaa tggaacttat
     1981 ctctcatgga gaccgtcata aatctataaa attctgcccg tatcgtacaa gaaaagtagg
     2041 aaattcgtag ggaaatacct atgttttctt aattttttcc aaatataatt ctaagccttg
     2101 cttgaaaacg aaggagaaat ttatgttgaa accaatgaaa ccaaagcaaa cttttccaaa
     2161 taacactatt ctgtggattc tggtgggagt ccttgttatt ggcggcatta ttgccattgt
     2221 gtctgcattt agtgggggag gaaagaatgc agaagatgaa gttaacgcgg tatacactaa
     2281 tgcggcagcg acccttgcta ttcagcagat gaccttgcag gcggcttccc cgtctgtaac
     2341 cccaactgtt gcaaccccaa cagggacagt gaccttcact ccacagcccc ttgccaccct
     2401 cccaacatcg caggggattt cgaccagttc ttcctcttcg tctggcggct ctggcgcggt
     2461 gggatgtaac aactcggtct ttgtctcaga tgtgaccgtg ccggatgata cggtaatgac
     2521 ccccggacag tcttttacca agacttggaa attgcaaaac accggctctt gtccgtggac
     2581 aacgtcgttc aaagttagct ttgtttctgg caatgcgatg agcgggacta ctactgctct
     2641 tactactacc gttcaagctg ggcaatctgg tgatatttca gtagctatga aagctcctgc
     2701 aactacaggt gacgccatcg gttattggat tttgaccaac gatagcggcc agaattttgg
     2761 ctcttctttc tatgttaaga tcaaagtagg tggcacttct acaacaggta cggtcactgc
     2821 aacggctact gccacggcta caacagcggc tgtaaccgct cctactgctg ccagcaatgt
     2881 tgcaatcaca ttgacttgta ctcttggtgg cacaaacggc acacaaaaag aacatgcagg
     2941 tacattaact tggaccgata attcaaataa tgaaaccggt ttcaatatct acattaattc
     3001 agtatttgcc gcaacagtag cagcaaatac tacatcgttt ggtgtgcctt cagggacatt
     3061 ttatgacaca ggtgttactt cgacctatgc tgttgaagct gtcaatggga gcactaaagc
     3121 agctcaggca actgtcactg taagttgccc ataatgcaat cgcattctta agaataaaaa
     3181 gacctgcaaa ctaattgcag gtctttttat ttattccact aactttgcca aactatcagc
     3241 aatgatgctc ggattccact ctttcgcttc ttcgagtttg gaaaccccgc tcaatacgaa
     3301 tgcggttggg cagcccacag cctgacctgc cgcaatatcc gtttcgaggc ggtcgccaac
     3361 gaccaaggtt tcttcttttg atgttcccaa ccgttcgagc gaaagctcca ttaagaaggg
     3421 gaaaggtttg ccagcgacca ttggctccac gcccgtagcc gatgtgacca cagagagcca
     3481 agacccagaa ccggggattt ctccgcgcgg agtggggaag gtgcggtctg tattagtcgt
     3541 gtaaaacgga cgacctgctc gcaccagtaa agtggcttcg gcaacttttt ggaagctaat
     3601 gccacggtca atgcccatta caacagcttc tgcttgatga gcatcctcca cgccaacgac
     3661 ttcaaacccc ttttcttcca acgccacacg gatgccattt tcgccgatca taaaaatttt
     3721 cgttccacgc ggatgccgtt gtgccatcat aaaggcgata ccaagcgcag aagtcacaac
     3781 ctgttccgct tccacatcc
//
LOCUS       DAOQJE010000253         3785 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_15019_length_3785_cov_5.705898, whole genome shotgun sequence.
ACCESSION   DAOQJE010000253 DAOQJE010000000
VERSION     DAOQJE010000253.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3785)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3785)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3785
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15019_length_3785_cov_5.705898"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1034)
                     /gene="recA"
                     /locus_tag="PLT08_07900"
     CDS             complement(<1..1034)
                     /gene="recA"
                     /locus_tag="PLT08_07900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008094 - ATP-dependent activity, acting
                     on DNA [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HNE04427.1"
                     /translation="MARKKAASGDAAPSQNIDNAKRAVLDKAVGDILKRYGDGSIMRL
                     GEAQGMVTEVIPSGSLSLDIALGVGGVPRGRITEIYGPESSGKTTLCLHIVAESQKMG
                     GTAAFVDMEHALDPVYAARLGVDIDNLLVSQPDTGEQALEITETLVRSGAVDVIIVDS
                     VAALVPRNEIEGDMGDASMGMQARLMSQALRKLSGAINQTKTSVIFTNQLRQKIGVMF
                     GNPETTTGGQALKFYASLRLDVRRIQSIKMGEEVIGNRTRVKVVKNKVAPPFRTAEFD
                     IMFNEGISKAGDVLDLATKFEVIQKRGAFFSYGDIRIGQGRENAKDYLRSNPDLMGEI
                     EGVIRQKSLSG"
     gene            complement(1178..2299)
                     /locus_tag="PLT08_07905"
     CDS             complement(1178..2299)
                     /locus_tag="PLT08_07905"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HNE04428.1"
                     /translation="MKQCDTRLSLILMTFKRFLSSFFVLLFISSCTYDVSGTNTPVAA
                     IQTAAPGQTEVPFEAFITPSPQPFQFNLPTPGAEPITNWRPPLYPVPWAVGPNDHFYF
                     TRPIAADQTNWPIADYRYGGVFFGRDIVHTGVDIPSPEGTPIMAAGPGTVVWADWGLF
                     RETPGDETDPYGMAIAIRHDFGYNDQQLYTIYAHMSQMIAFVGQHVETGEVIGLVGST
                     GATTGPHVHFEVRFPHNSFFYTYNPELWIAPPQGWGVLAGRIMDEKGELIQQMEILVA
                     PERASRTFTVRTYGKGGAVNSDPYYQENFVLGDLPAGIYKVSFKYDEERVQTWVEVFP
                     GQVSYFTYKNKEGFTIAPPPAPTLDFVPESLPATPTPQP"
     gene            complement(2299..>3785)
                     /locus_tag="PLT08_07910"
     CDS             complement(2299..>3785)
                     /locus_tag="PLT08_07910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE04429.1"
                     /translation="IKQVTNTPQYDSAPSWSPDGQWLAFETYQNENLEIAVVNVETGE
                     TVPLTQHPASDHSPAWAPDGRNVAFISTRSGNSDVWLANLDLAGDDRYTNLSNTPFAS
                     ENAPLWNPNGTQLLWSSASSQNIGFSGLYIWNAQNPTRAAYWVGDGNRGTWNEAANQV
                     MAAVGAPNETYLTSYDLNGKLVLASIPLSGQVRGLTWGSVDLPNPLPDSFNQAAVETP
                     VALWAPVITPGGDVPNQRWSVVPVENIQAPFPQLHDLVDESFYALRQRASTDAGWDVL
                     ASVENTFVPLTTSLEPGMDEDWLYTGRAFAINSLMANAGWMVTLREDIGAQTYWRVYI
                     RCAVQDGSMGEPIHNSPWNLNARYDLDPRTYEQGGSYAPVPAGYWIDITALAQAYGWE
                     RQPSLPNWRTYYKGARFTTFANTGGLTWYQAMRELYPEQALVTPTKVLAPTFTPTNTP
                     TLTSTPRPTRTPRMTFTPSLSPTVTNTPLPPTATPTPPTVIPEG"
BASE COUNT          962 a          855 c         1107 g          861 t
ORIGIN      
        1 ccactgagcg atttctggcg gatgacgcct tcgatttcac ccatgaggtc aggattgctg
       61 cgcaggtagt ctttggcatt ttcacgtccc tgaccgatgc ggatatcgcc ataggagaag
      121 aatgcgccac gcttctgaat gacctcgaac ttggtcgcca agtcgagcac atcaccagcc
      181 ttggatatgc cttcgttgaa catgatatcg aattcggcgg tgcggaaggg aggcgcaacc
      241 ttgttcttta ccactttgac gcgagtgcgg ttaccgatga cttcttcgcc catcttgatg
      301 gattggatgc ggcgaacatc gaggcgcagg gaggcataga acttgagcgc ctgaccaccg
      361 gttgtagttt cggggttacc gaacattacg ccgatcttct ggcgaagctg attggtgaag
      421 ataacggaag tcttggtctg gttgatggcg ccgctcaact tacgaagggc ttgagacatg
      481 aggcgggctt gcatacccat gctggcatcg cccatatcgc cttcgatttc gttgcgagga
      541 acgagggctg caaccgagtc aacgatgatg acatcgacag caccagaacg gacgagggtt
      601 tcagtaattt cgagcgcctg ttcaccagtg tcgggctgag aaaccagtaa attgtcaata
      661 tccacgccaa ggcgggctgc atagacgggg tcgagggcat gttccatatc gacaaaggca
      721 gcggtaccgc ccatcttttg agattcagcc acaatgtgca aacaaagtgt ggtcttacct
      781 gaagactcag gaccatagat ttcggtgata cgcccacggg gaacgccacc cacaccgagt
      841 gcaatgtcca atgacaatga accggaagga atgacttcgg tcaccatacc ttgggcttca
      901 ccaaggcgca taatagaacc gtcgccatag cgtttgagga tatcgccaac agctttatct
      961 aatactgcgc gtttggcatt gtctatattt tgtgagggtg cggcatcacc ggatgcagct
     1021 ttttttctgg ccatgagttt gtctcctttt gttgcttaac cacgagagtt attttcaaaa
     1081 caaaggttaa gtgggttaag tatttcgaga gtatagcaca gttgttctaa acgtcaaggt
     1141 cgcgtgaggg attggttaca ttacaagcac atgccgatta tggctgagga gtcggcgtgg
     1201 caggcaagga ttcggggacg aagtctaagg ttggggcagg tggcggagct atagtgaagc
     1261 cttctttatt tttatatgta aagtaagaga cctgaccagg aaatacttcc acccaggttt
     1321 gcactcgttc ttcatcatac ttaaatgaga ccttataaat accagcgggt agatcgccca
     1381 aaacaaaatt ctcttgataa tacgggtctg agttgacagc gccaccttta ccataggtcc
     1441 gcacagtgaa agtacgagag gcgcgttcgg gggctacgag aatctccatc tgttgaatca
     1501 gctccccttt ttcgtccatg atgcgaccgg cgagtacgcc ccaaccttga ggtggagcga
     1561 tccacaactc tgggttgtag gtatagaaga aagagttgtg tggaaaacgc acttcaaaat
     1621 gaacatgagg tccggtggtg gcaccggtag aaccgaccaa accgatcacc tcccccgtct
     1681 ccacatgttg accaacaaaa gcgatcatct gactcatatg agcataaatg gtgtaaagct
     1741 gttggtcgtt gtaaccaaaa tcatggcgga tggcaattgc cataccatag gggtcggttt
     1801 catcaccggg ggtttcacgg aacaaacccc aatcagccca aaccacagtg ccgggtccgg
     1861 cagccataat gggagtgcct tcgggcgaag gaatatctac gccggtatgg acgatatcac
     1921 gcccaaagaa cacgccacca taacgatagt ctgcgatagg ccagttggtt tggtccgctg
     1981 caatggggcg agtaaagtag aaatggtcgt taggtcccac cgcccaaggc acagggtaaa
     2041 gaggcggacg ccaatttgtg atgggctctg cgccaggagt gggaagatta aattgaaacg
     2101 gctgaggaga tggagtgata aacgcttcaa acgggacttc ggtttgcccc ggcgccgcag
     2161 tctgaatcgc agcgacaggc gtattcgtgc cactcacatc atacgtgcaa gaggaaatga
     2221 atagaagcac aaagaaagaa gagagaaagc gtttgaaggt catcagtata agggacagtc
     2281 gtgtgtcgca ctgcttcatt agccttcagg gatgacagta ggcggagttg gagtggctgt
     2341 aggcggcaaa ggtgtatttg tgactgtagg cgaaagcgaa ggcgtgaagg tcatgcgtgg
     2401 agtgcgagtg gggcgaggag tagatgttag tgttggagta ttggtagggg taaaagtagg
     2461 agccaacacc ttagttgggg tgacaagggc ttgttcgggg tagagttcgc gcatggcttg
     2521 ataccaggta agcccacccg tattggcaaa agttgtgaag cgagcgcctt tgtaataagt
     2581 gcgccagttg ggtaaggagg gctggcgttc ccagccatag gcttgagcga gagccgtgat
     2641 gtcgatccaa tagccagcag ggacgggagc ataactacca ccttgctcat aggtacgggg
     2701 gtcgaggtcg taacgggcat tcaagttcca aggggagtta tgaatgggtt cgcccatgga
     2761 accatcttgt acagcacaac ggatgtaaac gcgccaataa gtttgggcac cgatatcttc
     2821 ccgcagagtg accatccaac cggcatttgc catgagcgag ttaatagcga aggcacgacc
     2881 agtgtagagc cagtcttcgt ccataccggg ctcaaggctg gtagtaaggg ggacgaaagt
     2941 attttctaca ctggcgagga catcccaacc ggcgtcagtc gaggcgcgtt ggcgtaaggc
     3001 ataaaaagat tcgtcgacga ggtcgtgcag ttgagggaag ggagcttgaa tattttcaac
     3061 cggcacaacc gaccaacgtt gattaggtac atctccgccg ggggtgataa ctggtgccca
     3121 taaagcaaca ggggtctcaa cagcagcttg gttgaaagaa tcgggtaagg ggttgggaag
     3181 gtcaacactg ccccaagtta atccgcgaac ctgcccgcta agtggaatgg aggcaagcac
     3241 aagtttgccg ttgagatcat aggaagtgag ataggtttcg ttaggagcgc ctacagccgc
     3301 catgacttgg tttgcagctt cattccaagt gccacggttg ccatcgccga cccagtaagc
     3361 cgcacgggtt gggttctgag cattccaaat gtataagccg ctaaagccaa tgttttgcga
     3421 tgaggcagat gaccataata attgggtgcc gttaggattc cagagcgggg cattttcaga
     3481 agcgaaaggt gtattgctaa ggttggtgta acggtcatcg ccggcaaggt ctagattggc
     3541 aagccaaacg tcactgttac cactgcgagt ggagatgaaa gcaacgttac gtccatctgg
     3601 cgcccaagca ggagaatgat ctgaggcagg gtgttgtgta aggggcacgg tctcgccggt
     3661 ttccacattg acgacggcga tctcgagatt ttcgttttga taggtctcga aggcaagcca
     3721 ttgtccatca ggtgaccaag atggagctga gtcgtattga ggggtgttgg tgacttgttt
     3781 aatgt
//
LOCUS       DAOQJE010000254         3782 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_15046_length_3782_cov_3.880333, whole genome shotgun sequence.
ACCESSION   DAOQJE010000254 DAOQJE010000000
VERSION     DAOQJE010000254.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3782)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3782)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3782
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15046_length_3782_cov_3.880333"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..206
                     /locus_tag="PLT08_07915"
     CDS             <1..206
                     /locus_tag="PLT08_07915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate
                     aminotransferase"
                     /protein_id="HNE04430.1"
                     /translation="MVIAVATEGDELVKGMADHVLWIPDAPWMLSPIVTVLPLQMLAY
                     HIATIRGLDVDQPRNLAKSVTVE"
     gene            292..723
                     /locus_tag="PLT08_07920"
     CDS             292..723
                     /locus_tag="PLT08_07920"
                     /inference="COORDINATES: protein motif:HMM:NF025793.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine/deoxycytidylate deaminase family
                     protein"
                     /protein_id="HNE04431.1"
                     /translation="MRPDWDSYFLKIAYAVSERSTCDRAFVGCVLVREKRILTTGFNG
                     SPAGQDHCDEIGHLMVDGHCVRTIHAETNAIIQAALHGVSTKGATCYVTHLPCINCTK
                     ALINAGIARIVYSVAYRSDENALNFLKAANIEVAQREFTPS"
     gene            complement(728..1255)
                     /locus_tag="PLT08_07925"
     CDS             complement(728..1255)
                     /locus_tag="PLT08_07925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE04432.1"
                     /translation="MFYEFTEEELRSNQRGFISPKQKEFLNRYGKSLIQSHRSGWPVI
                     IFFMVLGGGIILGMNLSNESTRRALFADPLNLVILCASIPLILGVYGLGAFWARRTAS
                     RFREPELRVAEGAIRWDDEYSDSVGRTYFLYVGKTEFKFAEDLSYTFPIGKRARIFYC
                     EGSQIKLILSHELLS"
     gene            complement(1259..1840)
                     /locus_tag="PLT08_07930"
     CDS             complement(1259..1840)
                     /locus_tag="PLT08_07930"
                     /inference="COORDINATES: protein motif:HMM:NF016581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HNE04433.1"
                     /translation="MTTSRQKILTHLKKTRSASAREIARALKLSAPNVRHHLSVLCSD
                     GRVEMTSLHNREGRGRPEKVYSLSAAALGDNLSALAEAVLTVASKTLSMEAVASRILD
                     AKQFAGQPINKRLALLVEALNEMHYQARWEAGAGGPRVIFGRCPYARVIDEHPEICKM
                     DEAMLKATLERDILPTRRAETQQGLCPFLFEIG"
     gene            2042..2356
                     /locus_tag="PLT08_07935"
     CDS             2042..2356
                     /locus_tag="PLT08_07935"
                     /inference="COORDINATES: protein motif:HMM:NF022643.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2834 domain-containing protein"
                     /protein_id="HNE04434.1"
                     /translation="MKKNIYLILTIVGFFLPYYFVFKFYTANEMSTSAALAQLVSTDW
                     GALFVADLTISVFAAWTFIYNEAKRLKMNYWWAYPIATMMVGLSFALPLFLYFRERHL
                     EQ"
     gene            2353..2565
                     /gene="iscX"
                     /locus_tag="PLT08_07940"
     CDS             2353..2565
                     /gene="iscX"
                     /locus_tag="PLT08_07940"
                     /inference="COORDINATES: protein motif:HMM:NF016284.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein IscX"
                     /protein_id="HNE04435.1"
                     /translation="MSLHWDATFAIAMELRRRHKDVNIEDVTLQQILDWTLELSEFED
                     DPALVNDDILYAIYQDWYEEHIHGRK"
     gene            2552..3049
                     /gene="nuoB"
                     /locus_tag="PLT08_07945"
                     /pseudo
     CDS             2552..3049
                     /gene="nuoB"
                     /locus_tag="PLT08_07945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000236330.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoB"
     gene            3196..>3782
                     /locus_tag="PLT08_07950"
     CDS             3196..>3782
                     /locus_tag="PLT08_07950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C"
                     /protein_id="HNE04436.1"
                     /translation="MATPASTSTLDASAQLSASLVTRFPDSVTADARPGFSGFIVKKE
                     NLVEVATALRDEFGFDLLSSVTGVDYIAENKMEVVYHAHRTTGGPGIVFKTQVDRVDP
                     VEVPSLIDVWAGADFQEREAWDLMGIKFVGHPDLRRILMWEGFEGHPLRKDYKEALFE
                     EETKPYKSRWPEGNHTYSELKNPFRDNIKLPEGFSL"
BASE COUNT          985 a          978 c          904 g          915 t
ORIGIN      
        1 gcatggtgat tgccgttgcc accgaaggcg atgaattggt gaaaggcatg gcagatcatg
       61 tgctttggat cccggatgcg ccgtggatgc tctcaccaat cgtgacagtg ttgcctttgc
      121 agatgttggc gtatcacatt gcaaccatcc gtggactaga cgtggaccag ccacgcaacc
      181 tagcaaaaag tgtaacggta gaataaaaac aaaaagacag cctggctatt gccaggctgt
      241 ctttttgata agcatatata atacccaaaa ctactaacct taggagaatc tatgcgcccc
      301 gattgggatt cctatttttt gaagatcgca tatgccgttt cagaacgcag cacctgtgac
      361 cgtgcctttg tgggatgtgt gcttgtacgt gaaaagcgga ttctaacgac tgggttcaac
      421 ggctcccccg ctgggcaaga tcattgcgat gaaattgggc acctgatggt cgatggacac
      481 tgcgtccgca cgattcatgc tgaaacaaat gcgattattc aggcagcgtt gcatggggtt
      541 tccaccaaag gcgcaacttg ttacgtcacc catttaccct gcatcaactg caccaaggcg
      601 ttgatcaacg ctggcatcgc ccgcattgtg tatagtgtgg catatcgcag cgatgagaat
      661 gcgttgaact ttctaaaagc agccaatatt gaggtagcac aaagagaatt tacacccagt
      721 taattttcta agataatagc tcatgcgaaa gaatcagctt aatctgagac ccttcgcaat
      781 aaaagatccg tgcgcgtttg ccgattggaa atgtgtagct caggtcttct gcaaatttaa
      841 actcagtctt tccaacgtat aaaaagtaag tccgcccaac tgagtcggaa tattcatcat
      901 cccagcggat tgcaccttct gccacgcgca gctctggttc acgaaagcga gaagcagtac
      961 gcctcgccca aaaggcgcct aagccataca cacctaaaat caaaggtata gatgcacaca
     1021 aaataactag gttgagaggg tcggcaaaga gcgcccggcg ggtactttca ttggaaagat
     1081 tcatgccaag aataattccg ccgcccaaca ccataaagaa aataatcacg ggccagccgc
     1141 tgcggtgaga ctggattaag ctttttccat atcggttcaa gaattctttt tgctttgggg
     1201 aaataaaacc acgctgattc gagcgtaatt cttcttctgt aaattcgtaa aacataattc
     1261 atccgatctc aaataaaaat gggcacaaac cttgttgtgt ctctgcacgg cgagtgggca
     1321 aaatatcccg ttccagtgta gctttcaaca tggcttcatc cattttacag atctcggggt
     1381 gttcatcaat cacccgtgcg tacgggcaac gcccaaatat cacgcgcgga cctccagcac
     1441 ccgcctccca acgggcttga taatgcatct cattcaacgc ttcgactaac aaagccaaac
     1501 gtttattgat aggttgacct gcaaattgct ttgcatccaa aatgcgactc gccaccgctt
     1561 ccatacttaa cgttttgctg gctactgtca aaacagcctc cgccaatgcg gagaggttat
     1621 ctcccaacgc cgccgccgaa agcgaataga ctttctccgg tctgcccctc ccctcgcggt
     1681 tgtggaggct ggtcatttcc acgcgcccat cagagcaaag cacagagagg tggtggcgca
     1741 cattcggcgc ggagagtttc aaggcgcgtg caatttcacg ggcagaagca gagcgagtct
     1801 ttttgagatg agtgaggatt ttttggcggg aagtggtcat attgtctata cgcaggtgtt
     1861 cagagtaggg aaagttcaaa gaaacagagg taggctcaag ttacgggcga agcgagggtg
     1921 tcatcctgat ggagtgatta tattcacttc tttatttatg taaatatatt tttgcataat
     1981 tcaattgacg tatttccatc ccgtggctat aatccgataa cttgtgaagg attacaccga
     2041 tttgaaaaag aacatctacc tcattctcac tatagtcggg ttcttccttc cttattattt
     2101 cgttttcaag ttctacactg ccaacgaaat gagcacatct gcagcgctgg ctcaattggt
     2161 ctccaccgat tggggcgcgc tttttgttgc tgacctgacg atctccgtct tcgccgcgtg
     2221 gaccttcatt tataacgaag ccaaacgctt gaagatgaac tattggtggg catatccgat
     2281 tgccaccatg atggtcgggc tttcattcgc tttaccgctc tttctttatt ttcgtgaacg
     2341 acatcttgaa caatgagcct gcattgggat gccacatttg ccatcgcaat ggaattgcgc
     2401 cgcagacata aagatgtgaa cattgaagac gtaaccctgc aacagatatt agattggacg
     2461 cttgaacttt cggaatttga ggatgatccc gcgctcgtga acgacgacat cctatacgcg
     2521 atataccaag actggtacga ggagcacatc catggaagaa aataaatacg aaggattgaa
     2581 cctgccgaac tacaacattc cggaggatat tcagaacgtg gtttcaatca ccacgatcga
     2641 ccgcctctac aactggggac gccgctggtc ggtatggccc ctgatgttcg gtttggcatg
     2701 ctgcgcgatc gaaatgatcg ccgcacaaac tgcacggtac gatatggcac gcttcggtat
     2761 ggaagtgatg cgccccaccc cgcgtcaggc agacatgatg ctggtctctg gcactgtcac
     2821 caagaagatg ctgccgtcca tcatccgctt atacaaccaa atgcccgagc cgaaatatgt
     2881 cgtggcaatg ggcgcttgcg cctccagcgg tggacctttc aaagaaggct acaacgtagt
     2941 ggctggaatc gataaattcc tgcctgtgga tgtgtacatt cccggctgcc ctcccacccc
     3001 gcaggcgttg atcgcaggca tgatgaaact gcaagagaag atcgacaagc aatccatttc
     3061 gaaagtacgt tggtacgaaa agggcaaggt cgactcaaac tacgtgccca tgccgatcct
     3121 cggaccggac ctcattgacg tgcgccgcat caaggaaatt gaagctgccg ccgcacccaa
     3181 acaggaaggt gcctaatggc tactcccgcc tcaacctcaa cccttgacgc ttcggctcaa
     3241 ctcagcgcaa gcctggttac tcgtttccca gactctgtca ccgccgatgc ccgccccggg
     3301 ttctccggct ttatcgtcaa gaaagaaaac ctcgtagaag ttgccaccgc cttacgcgat
     3361 gaattcggct ttgacctgct ctcctccgtt accggcgtgg actatatcgc cgagaacaaa
     3421 atggaagtgg tctatcacgc ccaccgcacc accggcggac ctggaatcgt cttcaagact
     3481 caagtagatc gtgttgaccc tgtggaagtg ccttccctca ttgacgtgtg ggctggcgca
     3541 gactttcagg aacgtgaagc ttgggatttg atgggcatca agtttgttgg tcacccagac
     3601 ctgcgccgta ttttgatgtg ggaaggtttc gaagggcacc cgcttcgcaa ggattacaaa
     3661 gaagccttgt tcgaagaaga aaccaaaccc tacaagagcc gctggcccga aggcaaccat
     3721 acctattccg aattaaagaa ccccttccgc gataacatca aactccccga aggcttcagt
     3781 ct
//
LOCUS       DAOQJE010000255         3766 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_15136_length_3766_cov_6.374832, whole genome shotgun sequence.
ACCESSION   DAOQJE010000255 DAOQJE010000000
VERSION     DAOQJE010000255.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3766)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3766)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3766
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15136_length_3766_cov_6.374832"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..415
                     /locus_tag="PLT08_07955"
     CDS             <1..415
                     /locus_tag="PLT08_07955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE04437.1"
                     /translation="HLQWDSAIIQHYSFEIRGSSQDICMVNALIEVQDNKVILVQPLG
                     TSSPLSPQKWADPDWGNEVFLCDYNHFTIPKIFAMLEKTLGNSPFAILETEFDSLYGF
                     ITQFEDGIYTSHGWLNFRDKNVYNEFQISNFKIES"
     gene            complement(441..1040)
                     /locus_tag="PLT08_07960"
     CDS             complement(441..1040)
                     /locus_tag="PLT08_07960"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014447.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNE04438.1"
                     /translation="METIRGKTSLTMDSTELHARLANYKRISLDLGTGDGKFAFCHAQ
                     NFPSHFFIGMDSCRENLREYSRTNLPNLLYVIANAQSLPHELHGRISHITINFPWGSL
                     LSSLLNGDSDFLCGLESALGSGAKLEVRLNGGALSEQGWSLEDGSERIFQNLAMAGWR
                     LDAPANMDTHALRNFPSTWAKRLAFGRDPRAIHISASLK"
     gene            1336..1407
                     /locus_tag="PLT08_07965"
     tRNA            1336..1407
                     /locus_tag="PLT08_07965"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1369..1371,aa:Arg,seq:cct)
     gene            complement(1662..2015)
                     /locus_tag="PLT08_07970"
     CDS             complement(1662..2015)
                     /locus_tag="PLT08_07970"
                     /inference="COORDINATES: protein motif:HMM:NF018057.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1048 domain-containing protein"
                     /protein_id="HNE04439.1"
                     /translation="MMSIKDIIEGKRQWRAHMSRVNALPQDYRIVYKEIQKYLFKVGI
                     VELTEGTNILSGIVDLFTEGAAMQKGVLEVTGNDVAAFCDNLLKNSKTYSDVYQEAVD
                     QEVKKAMKIAMTKTK"
     gene            complement(2022..2369)
                     /locus_tag="PLT08_07975"
     CDS             complement(2022..2369)
                     /locus_tag="PLT08_07975"
                     /inference="COORDINATES: protein motif:HMM:NF018057.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1048 domain-containing protein"
                     /protein_id="HNE04440.1"
                     /translation="MNFWEKITGNDLKQEFKTFESRVQKLPADYQAAWEEINGTLWLH
                     SDFTGHNIMSILSGVLDLFEETSADGQSVQETLGDDIKGFCSALASEAGAKSYRDKWR
                     DQLNNTIAKKLGG"
     gene            complement(2369..2695)
                     /locus_tag="PLT08_07980"
     CDS             complement(2369..2695)
                     /locus_tag="PLT08_07980"
                     /inference="COORDINATES: protein motif:HMM:NF015513.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HNE04441.1"
                     /translation="MQEDLTEMLKGVLEGCILEIISRKKTYGYEITRQLNTLGFTDVV
                     DGTVYTILVRLEKNKLVDIEKSPSEMGPPRKFFTLNNAGREELRKFWEKWKFVSSRID
                     KLQEKS"
     gene            complement(2885..>3766)
                     /locus_tag="PLT08_07985"
     CDS             complement(2885..>3766)
                     /locus_tag="PLT08_07985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE04442.1"
                     /translation="IHEVRNYLEEIEFNPKRLDDVEERLDLIHSLTRKYGGNIPAVIS
                     YGADAKKQLETITGAADRIAELEMQEAKFLEKLSKQGNVLSDKRKSAAETMSKGIELE
                     LDDLRMASAQFGVDFQTKADPNGIPVADGTRLAFDQNGIDRIEFLVAPNPGEGLKPLA
                     KVASGGETSRLMLGLKNVMARADEVPSLIFDEIDQGIGGRVGMVVGHKLWNLSRSHQV
                     FCVTHLPQLAVFGDQHYQVQKLVDKGRTLTRVEPLEGESRLLELSQMLGEVGEGTLRS
                     AHELLQTARQMVKESKK"
BASE COUNT          936 a          842 c          854 g         1134 t
ORIGIN      
        1 acaccttcaa tgggattctg cgattatcca acattatagt tttgagattc gtggatcctc
       61 tcaagatatc tgtatggtta atgccttgat tgaggttcaa gataataaag tcatattagt
      121 tcaacctttg ggtacaagct ctccactctc acctcaaaaa tgggcagacc cagactgggg
      181 gaatgaggtc tttctttgcg actataacca tttcactatc cctaaaatat ttgcaatgct
      241 tgagaagact ttagggaatt ccccgtttgc tattcttgaa actgaattcg attcgctgta
      301 tgggtttatt acacagtttg aagacggaat atacactagc catggatggc ttaattttag
      361 agacaaaaat gtttataacg aatttcagat tagcaatttc aaaatagagt cataggtcac
      421 gctttggcgt gacctatttt ttacttcaaa cttgcactga tatgaattgc tcgcggatct
      481 cgtccaaagg caaggcgttt tgcccatgtg ctcggaaagt tacgtaacgc atgtgtatcc
      541 atgttggcag gggcatcgag acgccagcct gccattgcaa gattttggaa gatgcgctct
      601 gacccatctt ccagcgacca gccttgttcg gataacgcgc ctccgttcaa gcggacctcg
      661 agcttggcgc ccgaacccaa agcggattca agtccgcaga gaaagtctga gtcgccattg
      721 agcagactgc tcagcaaact accccacgga aagttgatgg tgatatgtga aatccgtcca
      781 tgcagttcat gcggcaggga ttgggcgttg gcaatcacgt aaagcagatt tggtagattg
      841 gtgcgcgaat attctctcag attctctcgg cacgagtcca tgccaatgaa gaaatggctg
      901 ggaaagtttt gagcgtggca aaaggcaaat tttccgtcac ccgttccaag gtccagagag
      961 atgcgtttgt aatttgcaag tcgtgcatgc agttcagtcg aatccatagt aagggacgtt
     1021 ttgccccgaa ttgtttccat gtgaaacctc caatttgtcg agttgagatt gagggagaca
     1081 aattggagcg gggcggtgca cgagcgcgct taaatgcaaa tagacggttg cttcgtcagg
     1141 caccgtctag atcatcaggg ctaggttttt tacgcgttag gggaaatgca aggagcgcca
     1201 gtgtggcgca gaacaatgat tagcagggag ggcgtgtggt gttcatagat ttcatcatag
     1261 catgcatata aaagctcgtg tattaatttt tcctatgagt gctggctgaa attttttggg
     1321 tataatgcct ttatcgctct gatagttcaa tggatagaac aaggcactcc taaggcttag
     1381 atgtaggttc gattcctact cagggcacaa gttgtttata cgagtaaatt acctctaatt
     1441 tttttcttag caagaagtac ttgacttttg gaaatgctcg gtgtattatt acaaccgttg
     1501 acagccaggg tgcccccctc accctggctg tcaacatttt aatttccaga ctcaaataaa
     1561 aaaaacaggc gcttttgcaa aagcgcctgt tttttgtctg tagattttat aggtcctcat
     1621 gttgcggttg agtgaacaat tggctaaatc gtattcgcct tttactttgt tttggtcata
     1681 gcgattttca tggctttttt aacctcctgg tccacagctt cttgatagac gtctgagtac
     1741 gttttagaat ttttgagcag attatcgcag aaagcggcta catcattgcc ggttacttca
     1801 agcacgcctt tctgcattgc tgcaccctct gtaaaaagat caacaatgcc tgagaggata
     1861 tttgtccctt cggtcagctc aacaatccct accttaaaga ggtatttttg aatctcttta
     1921 taaacaattc gataatcttg tgggagcgca ttgacacgtg acatgtgtgc tcgccactgc
     1981 cttttccctt cgataatgtc ttttatactc atcatattct cctagccgcc taattttttg
     2041 gcgatggtat tgttaagttg atcgcgccac ttatcacgat aagactttgc ccctgcttca
     2101 cttgccaatg ctgaacagaa gcctttgata tcatcaccca aagtctcttg aacgctttga
     2161 ccgtctgctg atgtttcttc gaataagtca agtacaccgc taagaatcga catgatgttg
     2221 tgaccggtga aatctgaatg cagccaaagg gtgccattaa tttcttccca tgctgcttga
     2281 taatctgctg gtaatttttg gactcgcgac tcaaacgttt taaattcttg tttcaagtcg
     2341 ttgcctgtga tcttttccca aaaattcatt agcttttctc ctgtagcttg tcaattcttg
     2401 acgatacaaa cttccatttt tcccaaaact ttcgtagttc ttcgcgaccg gcattgttga
     2461 gtgtgaaaaa cttgcgtggc ggacccattt cagatgggct tttttctatg tccaccagtt
     2521 tatttttttc aagccgcact agaatcgtat ataccgttcc atcaacaaca tcagtgaaac
     2581 cgagggtgtt tagttgcctt gtgatttcgt agccgtaggt ctttttgcgg cttatgattt
     2641 caaggatgca gccttccagc acacctttga gcatttctgt taggtcttcc tgcatatttg
     2701 cctccagtct atatagtatt acttactact gctatatagt agcacgtagt agctaggctg
     2761 tcaatagctt ttattcttaa atttagctag tcttatgccg cctgcaaaaa caggctcttc
     2821 aagaaaacaa tgattttagg ggatgcctga ataaaaaatc gggcgatagt catcgcccga
     2881 ttttttattt tttgctttct tttaccattt gtcttgcggt ttgtaagagc tcgtgtgcgg
     2941 accgtaacgt cccttcaccg acttcgccga gcatttgcga tagctccagc aaacgagact
     3001 caccttcaag cggctctaca cgggtgagtg tgcgaccttt atcaacaagt ttttggactt
     3061 gataatgctg gtcaccgaag acagcaagtt gagggagatg agtcacgcag aagacttgat
     3121 gactgcgcga tagattccac agtttgtgac cgacgaccat acctacacgt ccgccgatgc
     3181 cctgatcgat ttcatcaaag atgagagaag gcacttcatc ggcgcgagcc atgacgtttt
     3241 tcagacccag cataaggcgt gaggtttcac caccagaggc aacttttgcc aacggcttaa
     3301 gcccttcgcc ggggttgggg gcaacgagaa attctatgcg gtcgataccg ttctggtcga
     3361 aagcgaggcg ggttccatcc gcaacaggaa tgccattggg atcagccttg gtttggaagt
     3421 ccacgccgaa ttgagcagat gccatgcgca gatcatccaa ctccagttca atgcctttgc
     3481 tcatggtttc agccgcagac ttgcgtttgt cagaaaggac attaccttgc ttggaaagtt
     3541 tctcaaggaa cttggcttct tgcatttcaa gctcggcaat gcggtctgct gcgccggtaa
     3601 tggtttcaag ttgtttcttt gcgtctgccc cgtatgagat gacagccgga atattgccgc
     3661 catacttgcg ggtcagagag tggatgagat ccaggcgttc ttcgacatca tccaaacgct
     3721 tggggttgaa ttcgatctct tctaaataat tacgcacttc atgaat
//
LOCUS       DAOQJE010000256         3757 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_15198_length_3757_cov_5.478660, whole genome shotgun sequence.
ACCESSION   DAOQJE010000256 DAOQJE010000000
VERSION     DAOQJE010000256.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3757)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3757)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3757
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15198_length_3757_cov_5.478660"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..220
                     /locus_tag="PLT08_07990"
     CDS             <1..220
                     /locus_tag="PLT08_07990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE04443.1"
                     /translation="RFMLDAYYKAVWQVQIAISVGFFLLLIGLTNYSSPASMGTFSIG
                     AGVALLMRFMVKKEEAKEQEHVKETKKK"
     gene            complement(351..824)
                     /locus_tag="PLT08_07995"
     CDS             complement(351..824)
                     /locus_tag="PLT08_07995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE04444.1"
                     /translation="MGDYNRSTKEVTFGEVPVNVIKSIQTYIAKHNLGDILANPTMCI
                     ISTSEKIKKGLFGGGPGPKLLIQTAILTDRWLILADNVDQNALYIKSMQLRDITVEDY
                     EQSSFYKMIPDTGMNLNGQFTDASELGSMFLPLGKDVAGEKFKSALIESVQKAKL"
     gene            complement(912..1913)
                     /locus_tag="PLT08_08000"
     CDS             complement(912..1913)
                     /locus_tag="PLT08_08000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M42 family metallopeptidase"
                     /protein_id="HNE04445.1"
                     /translation="MKSLLKTLTETYAPSGYEDAVRKIIQKEVKPYADEIRVDAMGSL
                     IVRKKPTVAAQNSKRIMLAAHMDEIGVIVSHVDKNGFVRFASNGGVFGRYTLGARVRF
                     MNGVTGVIGFDKLEAVDQAPSLSKMYIDVGASSRSDCPVKVGDFGAFERSYMEMGNRL
                     VAKSMDDRAGVVVLIEALKSIKITPHDLYFVFTTQEEVGTRGAGTAAYSIDPEIGIAI
                     DVTGTGDTPASLKMSMELGKGPCVKVRDPGMLSDMRVVEWMASTAEKAKIPYQREVLL
                     VGSTDAKAIQVTRAGVMSGALSIPCRYIHSASEMVDFEDLKNSVKLLTAMLSKPISF"
     gene            complement(1917..2636)
                     /locus_tag="PLT08_08005"
     CDS             complement(1917..2636)
                     /locus_tag="PLT08_08005"
                     /inference="COORDINATES: protein motif:HMM:NF015534.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Type 1 glutamine amidotransferase-like
                     domain-containing protein"
                     /protein_id="HNE04446.1"
                     /translation="MNGLIALLGSGEYLPVMNEVDAYLLSNCGANGRKPRVVCLPTAA
                     GQEGDASVGRWMKMGVDHFTALGAEVQALKVIDKTTANDVNHAAAVKEADLVYFSGGN
                     PMYLYQTMKDSLVWQAAQEVFERGGVYAGCSAGAMILGREVPDFRMLGSRTTPVFGLV
                     PAAFILPHFDAIPIFGKPLLSVTRNRLHDGEVLIGIDEDTAIIGRANETWTVMGKAKA
                     HVFTKNESKSYADGEKFTLGK"
     gene            complement(2639..3685)
                     /locus_tag="PLT08_08010"
     CDS             complement(2639..3685)
                     /locus_tag="PLT08_08010"
                     /inference="COORDINATES: protein motif:HMM:NF017181.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE04447.1"
                     /translation="MLSIGSPQIKLLEKLCNAMAVSGDEGEVRRIVLEEVKPYADEVK
                     VDALGSVLVRKKGTGRKPLRVLLDAHMDEVGFMIVHDDGDGFYEFRIVGGIDERHLVG
                     KQVIVGKDHIPGVIGAKPIHLTDAGERSHTVDVESMRIDLGPDGKATVGDRATFATKF
                     KRVGPSIMSKSIDDRIGVAILIELLKNAPENIELCLAFSVQEEIGLRGATVAGYYFNP
                     DLAIAIDSTPARDLPDYEGRENYTYNTKLGLGPSIYPAHSPVISDPRLVSFLSEVGTK
                     HKIPYQFRQPGGGGTDAGAIQQAHSGIPVVSVSVPHRYTHSPISVSRVDDWKNTLNLL
                     HVALQEMTPALLKR"
BASE COUNT          923 a          964 c          926 g          944 t
ORIGIN      
        1 ccgcttcatg ctggacgcgt actacaaagc cgtttggcag gtgcaaattg ccatttcagt
       61 tggtttcttc ctcctactta tcgggctgac gaattattcc agccctgctt ctatgggcac
      121 attttccatt ggcgcagggg tagcgttgct aatgcgtttt atggtcaaga aggaagaagc
      181 aaaagagcaa gaacacgtca aagagacgaa gaaaaaatag gaaagatgaa agaaaaaggc
      241 ttgaacgata ccgttcaagc ctttttcttt acaagtatgt cacactgagc ggagcgaagg
      301 gtctcttaca ttatcgtaga gattcttcgc tatcgctcag aatgacgtca ttataacttc
      361 gccttctgca cggactcgat caacgcggat ttgaattttt ctccagcgac atctttccct
      421 agcggtaaaa acatcgaacc caattcgctg gcatcagtga attgtccgtt taagttcata
      481 cccgtatcgg ggatcatttt gtagaatgaa ctttgttcgt aatcttccac cgtaatatct
      541 cgcagttgca tggatttgat atacagcgca ttctggtcga cgttatcggc gaggatgagc
      601 cagcggtcgg tgaggatggc ggtttgaatc aacagtttcg gacccggtcc gccgccgaac
      661 agtccctttt tgattttttc agaagtggag ataatgcaca tagttgggtt agcgagaata
      721 tcacccagat tatgttttgc aatataagtt tgaatgctct ttataacgtt gacaggaact
      781 tccccaaatg taacttcttt tgtggaacgg ttgtaatcac ccatagtatt tacctttaaa
      841 tccgtaaggg cgacccactt ggtttaagca agatgacttg ctcttaccaa aagggtcgcc
      901 cctacaatta attagaatga aattggtttg ctcaacatag cagtgagcaa ttttactgag
      961 ttcttcaaat cttcgaaatc caccatctct gaggcagagt ggatgtagcg gcacggaatg
     1021 gacagcgcac cagacatcac accggcacgg gtaacttgaa tggctttcgc atcggtgctg
     1081 ccaaccaaca agacttcacg ctgatagggg attttcgcct tttctgcggt acttgccatc
     1141 cattcgacca cgcgcatatc tgagagcatg cctgggtcgc ggaccttcac acagggaccc
     1201 ttgcccaact ccatagacat tttcaatgaa gccggcgtat cgcctgtgcc agtcacatcg
     1261 atggcgatgc caatctccgg gtcaatgctg taagcggcgg ttccagctcc gcgagtgccg
     1321 acttcttcct gcgtagtgaa cacgaaatac aaatcatgcg gtgtgatttt gatggacttc
     1381 aacgcttcga tcaataccac cacgcctgcg cggtcatcca tagactttgc aacaaggcga
     1441 ttgcccattt ccatatacga gcgttcaaag gcaccaaaat caccgacctt caccggacaa
     1501 tcactgcggc tggatgcacc cacgtcaatg tacattttgc tgagcgaagg cgcttggtct
     1561 acggcttcta atttgtcgaa gccgatcaca cccgtcacgc cattcatgaa gcggacgcgt
     1621 gcgccgagcg tgtaacgacc gaagacaccg ccattgctgg caaaacggac gaagccgttc
     1681 ttgtccacat ggctgacgat gacgccgatt tcatccatat gagccgcgag catgatgcgt
     1741 ttggaattct gcgctgccac ggttggtttc ttgcggacga tgagactgcc catcgcatcc
     1801 acacgaattt catcggcgta tggcttcact tccttttgaa tgatcttgcg gacggcatct
     1861 tcatagcctg aaggcgcata ggtttcagtg agggtcttga gtaatgattt cattggttat
     1921 tttcccaaag tgaatttttc accatctgca taacttttgc tttcgttctt tgtaaacaca
     1981 tgggctttgg ctttgcccat tacggtccag gtttcgttgg ctctgccgat gatggctgta
     2041 tcttcatcaa tgccgatcag cacttcacca tcatgcagac gattgcgcgt gaccgagagc
     2101 aaaggcttgc cgaagattgg gatggcatca aaatggggga ggataaatgc cgctggaacc
     2161 aaaccaaaca ccggcgttgt gcgtgagccg agcattcgaa aatctggcac ttcacgcccg
     2221 agaatcatgg ctcctgcaga acaacccgca tagactccgc cgcgttcaaa cacttcttgt
     2281 gccgcctgcc aaacgagact atctttcatg gtttgataga gatacatagg attcccgccc
     2341 gaaaaataga cgaggtccgc ctctttcacc gccgcggcat gattcacatc attggcagtt
     2401 gtcttgtcga taactttgag cgcctgtacc tctgcaccga gggctgtgaa atgatccacg
     2461 cccatcttca tccagcgacc aacactggcg tcgccttctt gtcctgctgc cgtgggcagg
     2521 caaactacac gaggcttgcg tccattcgcg ccgcaatttg aaagcaaata ggcgtctacc
     2581 tcgttcatca cgggtagata ttcgccggaa ccgagtaaag caatgagtcc gttcatagtt
     2641 atctctttaa taaagctggg gtcatttctt gtaaagcaac atgcaataag ttcaacgtat
     2701 tcttccaatc atccacacgc gaaacgctga taggactatg ggtgtaacga tgcggcaccg
     2761 aaaccgaaac gactggaatg cccgaatgcg cttgttgaat ggcgcctgca tctgtaccgc
     2821 cgccaccggg ctgacggaac tggtacggaa ttttgtgctt ggtacccact tccgagagga
     2881 agctgacgag acgcggatcg ctgataacgg gggaatgagc cgggtaaatg gaaggaccta
     2941 accccagctt ggtgttgtag gtgtaatttt ctctaccttc gtaatcgggc aagtcgcgtg
     3001 cgggcgtgga atcgatggcg atggcgagat caggattgaa ataataacca gccacagttg
     3061 cgccgcgcag accgatctct tcttgcacac tgaatgcaag gcaaagctcg atgttttcag
     3121 gggcgttctt gagcagttcg atcagaatcg ccacgccaat gcggtcatcg atagacttgg
     3181 acatgatgga tggacccaca cgcttgaact tcgttgcaaa cgtagcgcgg tcaccgactg
     3241 tggctttgcc atctggaccc aagtcaatac gcatggactc gacgtccacg gtgtggctgc
     3301 gttcgccagc atcggtgaga tgaatgggct tggcaccgat cacgccggga atgtgatctt
     3361 tgccgacgat gacttgtttg ccgactagat gccgctcgtc aatgccgcca acgatgcgga
     3421 actcatagaa gccgtcgccg tcatcatgca cgatcatgaa gccgacttcg tccatgtggg
     3481 catcaagtaa cacgcgcaag ggtttgcgcc cagtgccctt tttgcggacg agtacgctgc
     3541 cgagcgcatc cacttttact tcatcggcat agggcttgac ttcttccaag acaatgcggc
     3601 gcacttcgcc ttcatcgcca gagacagcca tggcgttgca tagtttttcg agtaatttaa
     3661 tttgagggga gccaatggaa agcatcaatt aatcccaaac gattctttca agaaagtccg
     3721 cttcgagcga agcgacgaat tcagccatca aacgacc
//
LOCUS       DAOQJE010000257         3757 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_15199_length_3757_cov_5.220692, whole genome shotgun sequence.
ACCESSION   DAOQJE010000257 DAOQJE010000000
VERSION     DAOQJE010000257.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3757)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3757)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3757
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15199_length_3757_cov_5.220692"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            251..844
                     /locus_tag="PLT08_08015"
     CDS             251..844
                     /locus_tag="PLT08_08015"
                     /inference="COORDINATES: protein motif:HMM:NF018446.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022841 - potassium ion leak channel
                     activity [Evidence IEA];
                     GO_process: GO:0071805 - potassium ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TMEM175 family protein"
                     /protein_id="HNE04448.1"
                     /translation="MQKSRLEAFSDGVFAIVITLLILDIRFPEVPYNQFGQTLLSLLP
                     RILAYVMSFILIGLYWVVHHNSLHGMKKTDRGFLWLNILLLLFVSFIPFPTSLLGRYP
                     YQAGPIMFYGLTLIACNLVGYLMILYVWRHPHLAHAEFSPKYLQRHTPVYLVVNGLYL
                     CAVLVAHSLPLLSYLIYIAVVFGLIVLLPNLDDGLAA"
     gene            875..1459
                     /locus_tag="PLT08_08020"
     CDS             875..1459
                     /locus_tag="PLT08_08020"
                     /inference="COORDINATES: protein motif:HMM:NF018446.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005267 - potassium channel activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TMEM175 family protein"
                     /protein_id="HNE04449.1"
                     /translation="MSKGRMEAFSDGVIAIIITIMVLELRPPEEGTLAALLPLLPKFL
                     SYILSFVFIGIYWNNHHHLLQAAKGVNGSILWANMHLLFWMSTIPFTTSWMGEHFAST
                     PVAIYGAVLWITGLAYYILSRALIAHHGADSVIAKAIGRDEKGLRSLFLYTAAIVLAF
                     VSPWVSAGLYIVVAIIWFIPDRRIEEKVSNSKHD"
     gene            1574..2899
                     /locus_tag="PLT08_08025"
     CDS             1574..2899
                     /locus_tag="PLT08_08025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE04450.1"
                     /translation="MESQSLKVFKCPSCGGPLEPDKYSTTTKCPYCGAAVINPTSSTP
                     TGTLSDVTRLAKEGKLDEAARIYSKITGLSHENAMFSVKSMAGVREEEPASSNASPYS
                     NPYGMPQASPSYQAPPVPQPVYQPKVRARGGSCLSGIIRLIVLLSILGSAFPAIWKAL
                     QFQLPFELPFSTDGITIIPEPFAKEVMSINTGSFKDPRAIQVDGNDNILLFNYNSSDI
                     QMFDPQGNELSVMKITDADGDEINNSKMGVTSNGTIYVPGFQSIMSFSESGERLREIP
                     NTDQFFIIHTLLVGADDKLYVRSSSGILRFDENNKVDLFIPDATLEDLSGQTPSIGAL
                     GVDAQGNIYFSGTFNKDVLKFSPTGEFISSFGGDEFTSVRAIEIDSYGRIYIVDFSEV
                     RVYDSNFTYLGSIDGAFWGVDFDSQGYMYAVETNGDDVLKFEVKKPDAQ"
     gene            3032..>3757
                     /locus_tag="PLT08_08030"
     CDS             3032..>3757
                     /locus_tag="PLT08_08030"
                     /inference="COORDINATES: protein motif:HMM:NF012517.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HNE04451.1"
                     /translation="MSQPLNNQTRPFELGFYNFVDSGANPLTGEKKDPATVMKNMLEV
                     IELADQVGLDVFAIGEHHRVEYLASAPEVILGAAAARTKNIRLSTAVTVLSSDDPVRV
                     FQRFATVDLISNGRAEIMAGRGSFIESFPLFGYNLNDYDGLFDEKLRLLSKLNESTNI
                     TWSGNFRAPLNDLGIYPRPVQEKLPIWVAVGGTPESVVRAASMGLPMALAIIGGMPER
                     FAPYLDLYRQASAEYGFEPDTIPL"
BASE COUNT          904 a         1014 c          848 g          991 t
ORIGIN      
        1 cggcggtctc tgaaggcgac aaggctttac aagcaagcga tgcccagacc agcacagtga
       61 ctgccaagag gatgtttctt ttgttgttca tgaaaggctc caagaataaa gataagtttt
      121 tagaatacga ctaaagcaga gcggatttta ccgaagcctg cgcgtttgaa tatcacctaa
      181 aggtaagggg attttcgttt tgggctttat gctttacgat tagaagaata gaccaactgt
      241 tggagcagat atgcaaaagt cccgcctcga agcctttagt gatggggtct tcgccattgt
      301 tatcacttta ctgattctcg atattcgctt tccagaagta ccctacaacc aatttgggca
      361 gaccttgctt tctttattac cgcgcatcct cgcctatgtg atgagcttta ttctgatcgg
      421 cttgtattgg gtggtgcatc acaactccct gcatggcatg aaaaagaccg accgtggctt
      481 tctgtggctc aacatccttt tattgctgtt cgtgagtttc atccctttcc ccacctccct
      541 attggggcgc tacccttatc aagcgggtcc gatcatgttt tacgggctga ccctgatagc
      601 ctgcaatctt gtgggctatc tcatgatctt gtatgtctgg cggcatccgc acctggcaca
      661 tgcagagttc tctccgaaat acctgcaacg ccacacgccg gtctatctcg tggtcaacgg
      721 cttgtatctt tgtgcggttt tggtcgcgca ttccctgccc ctgcttagtt acttgatcta
      781 tatcgccgtc gtcttcgggc taatcgtcct cctgccgaat ttagatgacg gtttagccgc
      841 ctaaagaatc aaatcacctc aaggagaacc ctgtatgagc aaaggaagaa tggaagcttt
      901 tagcgatggc gtcatcgcca tcatcattac catcatggtc ttagagttac gtccgcccga
      961 agagggcacc cttgccgcat tactgccctt attacccaaa tttctaagtt acatcctcag
     1021 cttcgtcttc attggcattt actggaacaa tcatcaccac ctcttgcaag ccgccaaagg
     1081 cgtcaacggc tcgatcctgt gggcaaacat gcatctcctc ttttggatgt ccacgattcc
     1141 ttttaccacc agttggatgg gagaacactt cgcctccacg ccggtggcaa tttacggtgc
     1201 ggttctttgg ataactggct tggcatatta cattctgagc cgcgccctca tcgcccacca
     1261 cggcgcagac tcggtcattg ccaaagccat cgggcgggat gagaaagggc tgcgctcgct
     1321 ctttctttac actgccgcca tcgtcctggc atttgtctcg ccctgggtgt cggctggttt
     1381 atatatcgtc gttgccatca tctggtttat cccagatcgg cgcatcgaag agaaagtttc
     1441 caattcaaaa cacgactaat tcctttagcg aacgttcaag gcagtagccc aaaagagcga
     1501 tatatatcca accccaaaat cctttatgat gtaaagaaca gtcacgttat gtctttattt
     1561 ttcggagatg cccatggaaa gtcaatcgct taaggttttc aagtgcccat cgtgtggagg
     1621 tccgctcgag ccagataaat actccaccac tactaaatgt ccatattgtg gggcggcagt
     1681 catcaacccc acctcgtcaa caccgacggg aacgctctca gatgtcaccc gcctcgccaa
     1741 agaaggcaag ctggatgaag ccgcccgcat ctacagcaag atcacagggt tgagccacga
     1801 aaacgccatg ttcagcgtca agtctatggc tggggttcgg gaggaagagc ccgcttcgag
     1861 caacgccagc ccatattcaa atccatacgg tatgccgcag gcaagtccca gctatcaggc
     1921 gccgccagtg ccccagcctg tgtatcaacc caaagtccgc gcaagaggcg gttcttgtct
     1981 atctggcatt attcggttga tagtcctact ctccattttg ggaagcgcct tccctgccat
     2041 ctggaaagcg ctacaatttc agcttccgtt tgaattgcca ttctctacag atggcatcac
     2101 catcatccct gaaccttttg caaaagaagt catgtcgatt aacacgggtt cttttaaaga
     2161 cccacgcgcc attcaagtgg acgggaatga caatattctg ttgttcaatt acaacagttc
     2221 cgatattcaa atgtttgacc cacaaggcaa tgaactatcc gtcatgaaga tcaccgatgc
     2281 tgacggggat gaaatcaaca acagcaaaat gggcgtcacc agcaacggca cgatctatgt
     2341 acccggtttt caaagcatca tgagctttag cgaaagcgga gaacgcctta gagaaattcc
     2401 aaataccgac cagttcttta tcattcatac tctattggtc ggcgccgatg ataagttata
     2461 cgtccgctcc agttcaggta ttctgcgctt tgatgaaaat aacaaggtcg atttgttcat
     2521 cccagatgca accctcgaag acctatcagg tcaaactccc agtattggcg ctttgggtgt
     2581 agatgcacaa ggaaatattt actttagcgg caccttcaac aaagatgtgc ttaagttctc
     2641 tcctacgggt gaatttatca gcagctttgg aggtgatgag ttcaccagcg tgcgcgcaat
     2701 tgaaatcgac tcctatggtc gtatctatat tgtcgatttc tctgaagtac gagtgtacga
     2761 ttcgaatttc acctaccttg gcagtatcga tggcgcattc tggggtgttg actttgactc
     2821 acaaggctac atgtatgcgg tcgaaacaaa cggcgacgat gtcttgaaat ttgaagtcaa
     2881 aaagcccgat gcgcaatagc cctaatttct gcaatttata attctcaaag cgaccctctt
     2941 ctacgaaaaa gggtcgcttt ttagtaagac tttcgcctgt agctgcttct gtgtctctac
     3001 agaaaccatt aatactgaat cggaaatcaa aatgtctcaa cctttaaata accaaactcg
     3061 cccatttgaa ttaggctttt ataacttcgt tgatagtggc gcaaatccgc tgaccggtga
     3121 aaagaaagac cccgccacag tgatgaaaaa tatgctcgaa gtcatcgagc ttgccgacca
     3181 ggtcggcttg gatgttttcg ccattggcga acatcaccgc gtggaatatc tcgcctccgc
     3241 cccagaggtc atcctcggtg ctgccgccgc gcggacgaag aacatccgcc tctctaccgc
     3301 cgtaaccgta ctcagctccg atgaccccgt gcgcgtcttt cagcgcttcg ccacagtcga
     3361 ccttatttcc aatggtcgcg ccgaaatcat ggctgggcgc ggctctttca ttgagtcctt
     3421 tccgctcttt ggctacaacc tcaacgatta cgacggtctt ttcgatgaaa agttacgctt
     3481 gctctcgaag ctcaatgaat ccaccaatat tacatggtct ggcaacttcc gcgcaccact
     3541 aaatgacctc ggcatctatc cgcgcccggt gcaggaaaag ctcccaatct gggtcgccgt
     3601 cggcggcacg cctgagtcgg tcgtccgcgc ggcttcgatg ggtctgccca tggcgcttgc
     3661 cattatcggc ggcatgcccg agcgcttcgc tccctaccta gacctttacc gccaagcatc
     3721 tgccgaatat gggtttgaac cagatactat tccattg
//
LOCUS       DAOQJE010000258         3756 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_15205_length_3756_cov_6.979465, whole genome shotgun sequence.
ACCESSION   DAOQJE010000258 DAOQJE010000000
VERSION     DAOQJE010000258.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3756)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3756)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3756
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15205_length_3756_cov_6.979465"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..277
                     /locus_tag="PLT08_08035"
     CDS             <1..277
                     /locus_tag="PLT08_08035"
                     /inference="COORDINATES: protein motif:HMM:NF012296.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HNE04452.1"
                     /translation="YDVNADAKAVGETPEKLNPHRELEKGVYRSMIGFGSGPHRCAGE
                     HLALAETDVFIQKLLKLPGIRIESGPNIIRNDTVEGYEVNDLIVAVD"
     gene            344..772
                     /locus_tag="PLT08_08040"
     CDS             344..772
                     /locus_tag="PLT08_08040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE04453.1"
                     /translation="MKLNTKKEVWIARVIAWVLLCFISSGILFVTTFMVGPTLFVVPN
                     LTEKLAVAIYCPGAESTTLETGASTPTTSDPNGTTGHTVEITCTFPDGSTDIIDNGEY
                     AIASIGGMFGIGALIGLLISLPLFVVPFFLIRKRKEPSAL"
     gene            complement(817..>1095)
                     /locus_tag="PLT08_08045"
     CDS             complement(817..>1095)
                     /locus_tag="PLT08_08045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE04454.1"
                     /translation="NESNFTFSSINIVMQDKVKNISRSVTYNNFPIQTGCEGLTWVET
                     VPADASATVLGPKFDYNIRGNKLHVTVTVCSEKDMKGACSTRETNLVP"
     assembly_gap    1096..1195
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1196..1907)
                     /locus_tag="PLT08_08050"
     CDS             complement(<1196..1907)
                     /locus_tag="PLT08_08050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0070064 - proline-rich region binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HNE04455.1"
                     /translation="MSDLKRLMPFMVSILVVLACAIPSIPIQNVDTINTNVANTVIAG
                     LTQSVTATFPASSTPSETLAYTSTPTLTYLTARVPSQTPTFTPLPGTDTAIPLLTDTA
                     EPGLVKISVTRPTHCRSGPGKSYEIVGSLLVGMKADVIGRDPTSEYWYIPNPYVFTDY
                     CWVWGEYAEFEGNQLSVPVVMPPPTPTSTATTIPTVDFKLLGSGHHSCDKTHWMNITI
                     LNESNFTFSSINIVMQDKV"
     gene            complement(1946..2755)
                     /locus_tag="PLT08_08055"
     CDS             complement(1946..2755)
                     /locus_tag="PLT08_08055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE04456.1"
                     /translation="MKRFFSKKPEGQPTRGKTADNRLRRVDNFVLLYEPSLLTRTDSL
                     FAKSLFVDLGYGFDARTTLESAVRFRKVNPNLNILGVEIDKERVEAALPYADDKTFFR
                     LGGFNLPLQSGETVRLIRAFNVLRQYEEKDFSPAYERLAEYVLPGGLLVEGTSTPFGS
                     LWAANVARRVESAQWSFEALVFSTNFHTGFDVEEFQTILPKNYIHRVVKGELIFEFFE
                     AWKRSAAETSHTKIFGLKQWFIASAEALGQKGFQVDRHKKWLNKGYLIWYL"
     gene            complement(2815..>3756)
                     /locus_tag="PLT08_08060"
     CDS             complement(2815..>3756)
                     /locus_tag="PLT08_08060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HNE04457.1"
                     /translation="VEEIFEARKQPKGEATVAEIGGIIRIQQSEKYADMREVHIEHAE
                     MVHDEYAIPEDWKIVAKDESEVQAGEVLATLEKATIVSQHGGRVAVEKKDRKVIVSYE
                     QREEAILDIPNTSRILVRDGSHVEAGTPLTEGSLNPHRILKIQGRDACQMYLMTEVQK
                     VYRSQGQNIHDKHFEVIIRKMLSKVQVTRPGDTKYLPGDVVDRLEIRKTNEALIAEGR
                     QPARFSEVLLGVTKASLSTDSFLSASSFQHTIKVLASAAIGSTTDPLYGLKENVIIGK
                     LIPAGTGFIHGRFTPPGGEETAAPDSAEEMPDTTAGD"
BASE COUNT          973 a          891 c          847 g          945 t
ORIGIN      
        1 ctatgatgtc aatgccgatg ccaaagctgt aggtgaaact ccagagaaat taaatccaca
       61 ccgtgaattg gaaaaagggg tctatcgctc catgatcggc ttcggttctg gtccgcaccg
      121 ttgtgcgggt gaacatctcg ccttagccga aaccgatgtc ttcatccaaa agttgctcaa
      181 acttccgggc atccgcatcg aaagcggacc gaacatcatc cgcaacgaca cagtagaagg
      241 atacgaagtc aacgacttga tcgtagcagt cgactagaaa aaagacagcc tttgaaagag
      301 gctgtctttt tatataaatg atgatttcca tgagaggtga aacatgaaac ttaacacaaa
      361 aaaagaggtg tggattgccc gtgtgatcgc ttgggtcttg ttgtgtttta tttccagtgg
      421 gattttgttc gttacaactt tcatggtagg tcccacttta ttcgtcgttc caaacctcac
      481 tgagaaactt gccgttgcca tatattgtcc cggtgcagaa agcaccacgc ttgaaacagg
      541 tgcatctacg ccgaccactt cagaccccaa cgggaccaca ggtcacaccg tggaaatcac
      601 ctgcaccttc ccagatggct ccaccgacat catcgacaat ggcgaatacg caattgcctc
      661 catcggcggc atgttcggta ttggggcatt gatcggactg ttaatttcat tacctttgtt
      721 tgtggttccg tttttcttaa ttcgcaaaag aaaagagcca tcagctttat agcatctggc
      781 tctaaacaca taaggcactt catacttctt tgttaactaa ggcaccaaat tggtctcacg
      841 ggtactacat gcacctttca tatccttctc tgaacagaca gtaactgtca catgcagttt
      901 attaccacgg atgttgtaat caaatttagg accaagcaca gttgccgaag catctgcagg
      961 aaccgtctcc acccaagtaa gcccttcaca acctgtctga atagggaagt tgttgtacgt
     1021 cacagagcga gaaatattct tgaccttatc ttgcatcaca atattaatgg agctaaacgt
     1081 aaaattactc tcattnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1141 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnntgacc
     1201 ttatcttgca tcacaatatt aatggagcta aacgtaaaat tactctcatt aaggatcgta
     1261 atattcatcc aatgtgtttt atcacaagag tgatgcccgc tgccgagtaa cttaaagtca
     1321 acagttggaa tagtcgtcgc cgtactagtg ggagtaggcg gcggcataac aacagggaca
     1381 gataattgat tcccttcaaa ttcagcatac tccccccata cccaacaata atccgtgaat
     1441 acatagggat taggaatata ccaatattca gaagtcgggt ctcgcccaat tacatctgct
     1501 ttcattccta ccaaaagtga gccaacaatc tcatatgatt ttcccggacc ggaccgacag
     1561 tgagttgggc gagtaacgct gatctttacc agacccggtt ctgctgtatc tgttaataat
     1621 gggattgccg tatccgtacc aggaagtgga gtaaaggtcg gtgtttgaga aggcacacgt
     1681 gctgtaagat aagtcaacgt tggagtggaa gtgtatgcca gggtttcaga aggtgtacta
     1741 ctggctggga atgtagccgt tacactttgc gtcaacccgg caatcacggt gttagcgaca
     1801 ttggtattta tggtatcaac gttttgtatg ggaatactag gaatggcgca agccaaaaca
     1861 actagaattg aaaccataaa tggcataagt ctttttaaat cagacataga tgtctcctat
     1921 tacattccca attatgacac gaaaatcaaa gataccagat caagtaccct ttgttcagcc
     1981 attttttgtg acgatccact tgaaaacctt tttgccccaa cgcctcagca gaggcaataa
     2041 accattgctt tagcccaaaa atcttggtgt gtgatgtctc tgcggctgag cgtttccatg
     2101 cctcaaaaaa ttcgaagatc aactcacctt ttaccacacg atggatatag ttcttgggaa
     2161 gaatcgtctg aaattcttcg acgtcaaacc ccgtgtggaa attagtggag aagaccaacg
     2221 cctcaaacga ccattgagcg gactccaccc ttcgagccac attcgccgcc caaaggctac
     2281 caaacggcgt ggacgtccct tccacgagaa gaccgcccgg caaaacgtat tctgccaagc
     2341 gttcataagc aggggaaaaa tccttctctt catattgacg caatacattg aatgcccgaa
     2401 tcaaacgcac tgtttcgccg ctttgcaatg gcaaattgaa gccacctaaa cgaaagaaag
     2461 tcttatcatc ggcatacggc aatgccgctt caactcgttc cttatcgatc tccacgccta
     2521 aaatatttag atttggattt accttccgaa agcgaacggc gctctcaagt gtggtgcgtg
     2581 catcaaagcc ataaccgaga tccacgaaca gggacttggc aaacaggcta tcggtccggg
     2641 tgagaagcga aggttcatag agaagcacaa agttatccac acgtcgcagg cgattatcag
     2701 cagttttgcc acgtgtgggt tgtccctctg gttttttaga aaagaaacgt ttcatcaaaa
     2761 cgattataca aaataaaaac gccccgaatt ttcattcgag gcgtttcggt ttacttagtc
     2821 gccagcggtg gtatctggca tttcttcggc agagtctgga gcagccgttt cttcaccacc
     2881 gggaggggta aagcgcccgt ggatgaagcc cgtacctgca gggatgagct taccgatgat
     2941 gacgttctcc ttcaagccgt agagcgggtc ggtggtggaa ccgatggcag cgctcgccaa
     3001 cactttgatg gtgtgctgga aggacgaggc agagaggaag gagtcggtgg agagagaagc
     3061 cttggtcaca ccgagcaaca cttcactgaa gcgagccggt tgtcttcctt ccgcgatcaa
     3121 cgcttcgttg gtcttgcgga tctcaagacg atccaccaca tcaccaggca agtacttggt
     3181 atcgccgggg cgagtgacct gcaccttgct caacatcttg cggatgatga cttcaaagtg
     3241 tttatcgtgg atattctgac cttgtgagcg atacaccttc tgcacttcgg tcatgaggta
     3301 catctggcag gcgtcgcgtc cttgaatctt caagatgcga tgcgggttga gagaaccttc
     3361 ggtgagcggt gtgcctgctt ccacatgcga gccatcacgg acgaggatgc gggacgtatt
     3421 cggaatatcg agaatggctt cttcgcgctg ttcgtaagag acgatgacct tgcgatcttt
     3481 cttctccacg gcaacacgtc cgccatgctg agatacgatg gttgcctttt cgagcgtagc
     3541 gaggacttca ccagcctgca cttcagattc gtctttggca acgatcttcc agtcttcagg
     3601 gatggcgtat tcgtcgtgca ccatttcagc gtgttcgatg tgaacttcgc gcatgtctgc
     3661 atatttttca gactgttgaa tgcggatgat gccgccaatt tcagcaacgg tcgcttcacc
     3721 cttcggctgt ttgcgcgctt caaagatttc ttccac
//
LOCUS       DAOQJE010000259         3754 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_15219_length_3754_cov_6.557718, whole genome shotgun sequence.
ACCESSION   DAOQJE010000259 DAOQJE010000000
VERSION     DAOQJE010000259.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3754)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3754)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3754
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15219_length_3754_cov_6.557718"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            168..2237
                     /locus_tag="PLT08_08065"
     CDS             168..2237
                     /locus_tag="PLT08_08065"
                     /inference="COORDINATES: protein motif:HMM:NF024883.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HNE04458.1"
                     /translation="MTNNIERHAKLLKASARAAKNITTILDPYELFQRTVDIICDEFG
                     FYYAGVFLVDDASQYAVLKAGRGDAGKAMLREEHKLAIGGNSMIGASIANKKGRIALD
                     VGEEAVFFENPHLPKTRSEMALPLIIADEVIGALTVQSTEEAAFHEEDIDALQTMADQ
                     LAVAIQNSKLHRQEERRSRLLKAANRVGKEVASLLDLDQLLPQTVNIICEAYGLYYAG
                     VFLLDEKGEYAVLRAGYGKAGKAMLSEGHKLRVGTDSMIGACVAMGEARIALDVGEER
                     VHFKNPHLPHTRSEMALPLIYGGKTLGAVTIQSSEERAFSDDDITTLLTMAEHLAVAI
                     NNAKLIDELKEAHNEILRNKVFEALTSASTEAIHWIGNKTLPISLTVSRLREEIADGK
                     VDVESLKEDLDMISESAAQIIQVKEQLIGAVREMKPRPVLFADVLQTAAMQRGIAQNA
                     LKVSIDPEAAYVIADSTQLTRAVGNILQNAVEAGAKSVKVRVHPTEERGVLEINIEDN
                     GSGMNDETQRKVWSPFYTTRGVSHHGLGLPAAMHVVSQSQGRIALVSEEGKGTTVALF
                     MPQGRVTSSEVQPGGVKNILLIDDNDDWSNLLTEMFKGSKVKLTQTTDLKKLPEADLI
                     LVDENIASAPLTDVLAALSKAGLASKTVVLTSAINPERVTQYLREGLKDVTVKPYSVN
                     ELNELLK"
     gene            2291..>3754
                     /locus_tag="PLT08_08070"
     CDS             2291..>3754
                     /locus_tag="PLT08_08070"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNE04459.1"
                     /translation="MRPRWRKVFHDLWDNFMRTALVVLSIAVGVFSIGVITGAYEIIS
                     NDMGESYAANNPANIELRMTNFNDDVLSSIRNFKGVQDAEGRRVINYRVHPEGSTQWS
                     TIDLIAVEDFSKNNVNLLVPILGKAEAGKDEIVLNKKNMDKVHATVGDYLVFELADGT
                     TKKLKVVGVVQDSSTGADDFLAPPFAFTVMDTLQTLQQPDLFNRAYLMVSDSPNDLTH
                     IRTLGADLKDKLEKNGTTVIRTRYSERNKHPMTDTINAVLYILMALGVLIVFLSSSLI
                     ANTLSALLSQHLRHIGIIKLVGGRNKQVFAMYLTLIMAFAVLSLLIAIPLGGWGAYAL
                     AAFIADNLNFGLLGYRIVPIAFFVQVAVGLLVPLFAGLAPVLNGSRISVLRALSNDVA
                     GDESKPHVAGGKPRVNWLDWALVRLTRILAARGMHMPRPFIISLRNTFRRTNRLLLTL
                     FTLTMGGAIFIAVFNVRVSLRDYTEQIGSYFRADVTLD"
BASE COUNT          928 a          823 c          968 g         1035 t
ORIGIN      
        1 tacattaccg gttgtgattt tgacggcata cgatgaagtg aacaaagcca aagatgctgt
       61 ttctaaaggt gccaatgcct ttttcgtaaa aggacgtgag caaggtttgg tggagttgat
      121 ccaaaaaatc ttgagtggaa attaattcac tttcaacaga ggaacttatg acaaataata
      181 ttgaacgaca tgcaaaatta ttgaaagcct ccgcgcgtgc cgcaaagaac atcaccacca
      241 ttcttgaccc ctacgagctt ttccagcgta cagtggatat catttgcgat gagtttggct
      301 tttactatgc cggtgtcttt ttggtagatg atgccagtca gtatgctgtg ctcaaagctg
      361 ggcgtggcga tgctggtaaa gccatgctgc gcgaagaaca taaactggcg attggcggca
      421 attccatgat cggtgccagc atcgcgaata agaaaggtcg catcgcgctg gatgtgggcg
      481 aagaagcagt gttttttgaa aacccacatt tgcccaagac ccgttctgag atggcacttc
      541 cgctcatcat tgccgatgaa gtgatcggtg cgcttacggt tcagtcgacc gaagaggctg
      601 ctttccacga agaagatatt gacgcactgc agaccatggc agatcagctt gcggttgcta
      661 ttcaaaactc caagttgcac cgccaagaag aacgccgctc tcgcttgctc aaggctgcga
      721 atcgagttgg aaaagaagtt gcatctcttc tcgacctcga ccagctctta ccgcagaccg
      781 tgaatattat ttgtgaagcc tatggcttgt actatgcagg cgtgttcttg ctcgatgaaa
      841 aaggtgaata tgctgtctta cgtgcaggct atggcaaggc tggcaaagcc atgctttctg
      901 aaggtcataa gttgcgcgtc ggtaccgact cgatgatcgg tgcttgtgtc gcaatgggcg
      961 aggcgcgtat tgcgttggat gtaggcgaag aacgggtgca tttcaagaat ccgcatttgc
     1021 cgcacactcg ctctgaaatg gcgcttccgc tgatttatgg tggcaaaact ctcggggcgg
     1081 tcaccattca atcttctgaa gaacgtgctt tcagtgatga tgacattact actctgttga
     1141 cgatggctga acacttggct gtcgcaatca ataatgccaa actaatcgac gaacttaagg
     1201 aagcccataa cgagatcctt cgcaacaaag tctttgaagc gctcacttcc gcttctaccg
     1261 aagccattca ctggatcggc aataagacgc ttcccatctc gctgacagtt tcccgcttgc
     1321 gcgaagaaat tgccgatggc aaagtggatg tcgaatcgct caaagaggat cttgatatga
     1381 tctctgagag tgctgcacag atcattcagg tgaaggaaca actgatcggt gccgtccgtg
     1441 agatgaaacc gcgcccggtt ctctttgctg atgtgcttca aaccgcagct atgcaacgcg
     1501 gcattgccca gaacgctttg aaggtatcca ttgaccctga ggctgcttat gttattgcag
     1561 actccactca gcttactcgt gcagttggta acattttaca aaatgctgtt gaagcgggtg
     1621 ccaagtccgt caaagtgcgg gtgcatccta ccgaagagcg cggcgtgctt gaaattaaca
     1681 tcgaagataa tggttctggc atgaacgatg aaactcagcg caaagtttgg tcgcctttct
     1741 acacgacacg tggtgtttca catcatggtt tggggttgcc tgcggcaatg catgtcgttt
     1801 cgcaatcaca gggtcgcatt gcactcgtca gcgaagaagg taagggaact accgtcgcgt
     1861 tgtttatgcc ccagggacgt gtgaccagtt ccgaggtcca gccgggaggc gtgaagaata
     1921 tcctgttgat cgatgataac gacgattggt caaatttgct cacagagatg ttcaaaggtt
     1981 cgaaagtcaa gttgacccaa acgacagact tgaagaaact ccctgaagcc gatctgattt
     2041 tggtggatga gaacatcgcc tcggctccgc tgaccgatgt gttggctgct ctttccaagg
     2101 ctgggttagc atcgaagacc gtggtcctca cctctgccat caaccctgag cgtgtgaccc
     2161 aatatctgcg cgaaggtttg aaagatgtaa cggtcaagcc gtattctgta aacgaattga
     2221 atgaactgtt gaagtaaagg aaagaaagtt gacaggtctt caagacctgt caactttgta
     2281 aaagagaaac atgagaccac gttggcgtaa agtatttcac gatttatggg ataacttcat
     2341 gcgtactgca ttggtggttt tatccattgc agtgggtgtg ttttccattg gggtaatcac
     2401 tggtgcgtat gagattatct caaacgatat gggagaatcc tacgcggcaa ataaccctgc
     2461 gaatattgaa cttcgcatga cgaatttcaa tgacgatgta ctgtcgtcta ttcgtaattt
     2521 caagggtgtt caagatgctg aaggcaggcg tgtgattaac tatcgcgtcc atcctgaggg
     2581 cagtactcaa tggtcgacca ttgacttgat tgcggtagaa gacttcagta agaataatgt
     2641 taatttattg gtgcccattt taggcaaggc agaagcgggc aaggatgaga ttgtcttaaa
     2701 caagaagaac atggataaag tccatgctac agtgggtgat tatctcgtct ttgagttggc
     2761 agatggaacg accaagaagt tgaaagtggt cggtgttgta caagattcca gtaccggtgc
     2821 tgacgatttt cttgcccctc catttgcgtt tacggtgatg gatacgcttc agacgctcca
     2881 gcagccagat ttattcaacc gtgcctattt gatggtgagt gattcgccca atgacctcac
     2941 tcatatccgc actttgggtg cagacctgaa agataaactg gaaaagaacg gtactaccgt
     3001 tatccgcact cgttattctg agcgcaacaa acatcccatg acagatacga tcaatgcggt
     3061 cttgtatatt ttgatggcat tgggtgtgtt gatcgtgttt ttatccagct cgttgattgc
     3121 taatacattg agtgctttgt taagccagca cttgcgtcat attggtatta tcaagttggt
     3181 gggcggtaga aataagcaag tctttgcgat gtatctaacg ctcattatgg cattcgctgt
     3241 attgtctctt ctaattgcta ttccattagg cggttggggg gcttatgcac ttgcagcgtt
     3301 tattgcagat aacttgaatt ttggtttatt gggttaccgc attgttccga ttgcattttt
     3361 tgttcaagta gccgttgggt tgttggttcc gctctttgct gggcttgccc cggttctaaa
     3421 cggttcgcga atttcggtgt tgcgtgcttt gagcaacgac gttgctggag atgaaagtaa
     3481 gccgcatgtg gcgggcggga aacctcgggt taattggctc gattgggcat tggtgcgcct
     3541 gacgcgtatt ttagctgcac gtggaatgca tatgccacgt ccgttcatta tttcgctgcg
     3601 gaatactttc cgccgtacga atcgcctttt attgacgctc tttacgctaa ccatgggcgg
     3661 cgcgattttt attgcagtat tcaatgtccg tgtcagtttg cgtgactaca ctgaacaaat
     3721 cggcagctac ttccgcgcag atgtcactct tgat
//
LOCUS       DAOQJE010000260         3753 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_15225_length_3753_cov_5.813683, whole genome shotgun sequence.
ACCESSION   DAOQJE010000260 DAOQJE010000000
VERSION     DAOQJE010000260.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3753)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3753)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3753
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15225_length_3753_cov_5.813683"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..851
                     /locus_tag="PLT08_08075"
     CDS             <1..851
                     /locus_tag="PLT08_08075"
                     /EC_number="4.1.1.31"
                     /inference="COORDINATES: protein motif:HMM:NF012532.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008964 - phosphoenolpyruvate carboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA];
                     GO_process: GO:0015977 - carbon fixation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxylase"
                     /protein_id="HNE04460.1"
                     /translation="AGSINGKFRVTEQGEIIASRYANPGLAHRHLEQIASAVILASAP
                     HKEEPIPAKWRETMETISNAGFRAYRSLVYETPGFIEFWEAATPLDEIKRLQIGSRPA
                     SRAKAGAVNQIRAIPWVFSWMQSRFNLPSWYSLGSGLEAVADLSLLQEMYDGWLFFRT
                     LLNNSEISLLKADMDISALYVSLVPDQKLANDVFKTIRTEYERTCDAVLRISRHQNLL
                     ELEPLTQQAVQLRNPYVDPLNYIQVETLQRLRKLDDQDGDEAKSLREVMALTINGIAA
                     GLRNTG"
     gene            912..1484
                     /locus_tag="PLT08_08080"
     CDS             912..1484
                     /locus_tag="PLT08_08080"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HNE04461.1"
                     /translation="MVKIENSKRTILNLGETLLQDKGFNGFSYANIAAELGVKNAAIH
                     YHYPTKEDLGIAVMQRYRERFKLWINNARVKDLTPEGKLDWFLSIYTNMRADQGKICL
                     VGSMEAEFNSIPEGLQSEVQGLHKELLIWLQSTLTEGRDAGAFAFKGEPADKAAMLLS
                     TLQGALQMARALGAKKFHAVIEQIKLDLLK"
     gene            1538..2344
                     /locus_tag="PLT08_08085"
     CDS             1538..2344
                     /locus_tag="PLT08_08085"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HNE04462.1"
                     /translation="MNALEPKVDSQAVIKKVYRAFFGPSKYEVKSSDEAILARGKNYR
                     IPFEGGELAVTTWGESGPSILLMHGWGGARAQMTGFVEPLLAAGYRVVAYDQPAHGES
                     DGKMTNVLEISPTMDLVMQREGKFDAIIAHSFGTIITSYALVKRNFPMPSKLIYLGAF
                     NRLLDSLPRFQVLANLPDEVMHGLRNMITENFGKDVLEEIVNENLVKQIHIPALMFHD
                     RSDNVTPVEDSRAIASAWSEAQYIETSGLGHRGALQSAEIHEKIIDFLKA"
     gene            2595..>3753
                     /locus_tag="PLT08_08090"
     CDS             2595..>3753
                     /locus_tag="PLT08_08090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HNE04463.1"
                     /translation="MAGMERFTQRARRVLSLAHQEAERARQNSIGTEHLLLGLMDEEG
                     GVAGRVLRELGMSSDRVREVVARISSGSPTFDPNRIELAPETQQVLEFAVDEAKRLGH
                     HYIGTEHILLGLVRVDSTALEALRRLGVTPDQIRRQTRRVLNESASSSSPTPAGPQPG
                     KTAAGANNPKTPLVDQLATDLTSKAEEKKLDPVIGRQMEIERVIQILARRTKNNPALI
                     GEPGVGKTAIVEGLAQRIIDGDVPAPLMNKRLLQLDVGSLVAGTMYRGQFEERLKRII
                     DELKQSGAILFIDEVHMLVGAGAAGSSVDAANILKPALSRGELQVIGATTLEEYRKHI
                     ESDAALERRFQPVQVDEPSEEETIQILKGIRGAYEEHHHLVISDEALEAAAH"
BASE COUNT          980 a          895 c          949 g          929 t
ORIGIN      
        1 ctgcggggag catcaatgga aagttccgtg ttacagagca aggcgagatc attgcttcgc
       61 gctatgccaa ccctggtttg gcacatcgtc acttggagca gatcgccagc gcggtgatcc
      121 ttgcatctgc acctcataag gaagaaccca ttcctgccaa atggcgcgaa accatggaga
      181 ccatttcgaa tgcgggtttc cgggcatatc gctcccttgt gtatgaaacc ccgggcttta
      241 tcgagttttg ggaagcagca actcctttag atgaaatcaa acgcttgcaa attgggtcac
      301 gcccggcatc gcgtgccaaa gcgggggcgg tgaatcagat ccgcgcgatc ccgtgggtct
      361 tctcatggat gcagagtaga ttcaacttac cgagctggta cagcctcggg tccggtttgg
      421 aggcggtggc tgatctttca ctccttcaag aaatgtatga tggctggcta ttcttccgca
      481 ccttgttgaa taattccgag atttcattac tcaaagcaga catggatatt tctgctttat
      541 acgtcagcct tgtgcccgat caaaaacttg caaacgatgt tttcaaaacc attcgcactg
      601 aatacgaacg gacatgcgat gctgtattgc gaatcagccg ccatcaaaac ttgctggaat
      661 tggagccgct cacgcaacaa gctgtgcaac ttcgcaaccc atatgtggac ccgttgaact
      721 acatccaggt agaaaccttg cagcgcttgc gaaaactgga tgatcaggat ggagacgagg
      781 caaagtcttt gcgtgaagtc atggcattaa cgatcaatgg cattgctgca ggcttgagga
      841 ataccggata gtttgtgact ctaatgggga aggcttgaca acttactaac tagtaagtaa
      901 aatttttacc aatggtcaag atcgagaact ccaaacgcac aattctgaac ctgggcgaaa
      961 ccttattgca agacaaaggt ttcaatgggt ttagttatgc caacatcgct gcagaattgg
     1021 gtgtgaaaaa tgctgctatt cattatcact accctaccaa agaagatttg gggattgcgg
     1081 ttatgcagcg ttatcgcgag cgtttcaagt tatggatcaa taatgcccgc gtcaaagacc
     1141 tgacgccaga ggggaaactg gattggttct taagtattta cacaaacatg cgtgcagacc
     1201 aaggcaagat ttgccttgtc ggttccatgg aagcagaatt caattccatt cccgaagggt
     1261 tacaaagcga agttcaaggc ttacataaag aattgctaat atggcttcag tccactttga
     1321 ctgaaggcag agacgctggc gcctttgcct tcaaaggaga gcccgcagat aaagccgcaa
     1381 tgcttttatc gaccctgcaa ggcgcattac aaatggcgcg tgccttgggc gctaagaaat
     1441 tccacgctgt gatcgaacag attaagttag atttactcaa ataaattttt tttgtcaata
     1501 aaacttacta ggtagtaagt tttcaaaagg agaaactatg aatgctttag aaccaaaagt
     1561 tgattcgcaa gctgtaataa agaaggtgta tcgcgccttt ttcggaccga gcaaatatga
     1621 ggtgaaatca tcggatgaag ctattcttgc tcgaggaaag aattatcgca tccccttcga
     1681 gggcggtgag ctggctgtca ccacatgggg cgaaagcgga ccatccatct tattaatgca
     1741 tggttggggc ggtgcccgcg cgcaaatgac tggttttgtt gaaccgcttc ttgcggcagg
     1801 atatcgagtg gttgcatacg atcaacctgc tcatggcgag tcggatggca agatgaccaa
     1861 tgtgcttgag atttccccca ctatggatct ggtgatgcaa agagaaggta agttcgatgc
     1921 tattattgcc cattcatttg gcaccattat tacttcgtat gctctggtaa agcggaattt
     1981 ccctatgcct tctaaattga tttatctggg tgcattcaat cgcttgctgg attcgcttcc
     2041 gcgttttcaa gtgctggcaa acctgccaga tgaagtcatg cacgggttga gaaacatgat
     2101 caccgaaaat tttgggaaag acgtgttgga agagattgtc aacgaaaatc tggtaaagca
     2161 gattcatatt cctgccctga tgttccatga ccggtccgat aatgtcacgc cggtggaaga
     2221 tagtcgtgcc attgcatccg catggagcga ggcacagtac atcgaaacaa gtgggttggg
     2281 tcaccgtggg gcattgcaat cggcagagat tcatgaaaag ataatcgatt tcttaaaggc
     2341 ataataaaaa ggacccagcc gggtcctttt tattttaggc ttgatgtaag aatcgtgatt
     2401 tcaagagtat cagtatgctt caattccaca tctttcaaat accctatcca ttcggctagg
     2461 tatttcgtaa aagatgaggg tttttcgata aaaagccaag attgtccgct tcttgtaaga
     2521 aaactctaac ttttttccta cgcggggaat tgggcttttt ggtagaatcc ctcaaaggaa
     2581 tttaggagaa aattatggct ggcatggaac gttttacgca gcgagcaagg cgtgtactca
     2641 gcctcgccca tcaagaggca gagcgcgccc gtcaaaatag tattggtacc gagcaccttc
     2701 tcctcggatt aatggatgaa gaaggcggtg tggctggacg tgtgctgcgc gaattgggca
     2761 tgtcctcaga ccgtgtgcgt gaagtggtgg cacgcatctc aagcgggtct ccaacctttg
     2821 acccgaaccg tattgaactg gcacccgaaa ctcagcaagt tcttgaattt gctgtggatg
     2881 aagccaagcg cttggggcat cactatatcg gcactgagca tatcttgctt ggtctcgtgc
     2941 gtgtggactc tactgctctt gaagccctgc gtcgcctcgg tgtgactcca gatcagattc
     3001 gtcgccaaac ccgccgcgta ctgaacgagt ccgcttcatc ttcctccccg acgcctgccg
     3061 gacctcaacc gggcaaaacc gccgctggag ccaacaaccc caagaccccc ctcgtcgatc
     3121 aactcgccac cgacctgacc tccaaagccg aggaaaagaa actcgacccg gtcattggtc
     3181 gccagatgga aattgaacgc gtcattcaaa tcttggcgcg ccgcaccaag aacaatcccg
     3241 ccctgattgg tgaacccggt gtgggcaaga ccgccatcgt ggaaggtctc gcccaacgca
     3301 tcatcgacgg cgatgtgcct gccccgctca tgaacaagcg cctgcttcaa ttggatgtag
     3361 gttcacttgt cgcgggcacc atgtaccgtg ggcaattcga agaacgcctc aagcgcatca
     3421 tagatgagtt gaagcaatca ggtgcgattc tatttataga tgaagttcac atgttggttg
     3481 gggcaggagc cgcaggttct tccgtcgacg cagccaatat cctcaagcca gcgttgtcac
     3541 gtggtgagct gcaagttatc ggcgcgacca cgttagaaga atatcgcaag cacatcgaat
     3601 cagatgctgc gctcgaacgt cgtttccaac ctgtgcaggt agatgaaccc tctgaggaag
     3661 agacgatcca aattctcaag ggcattcgtg gtgcctacga agaacaccat cacttggtga
     3721 tctctgatga agcactggaa gcggctgctc atc
//
LOCUS       DAOQJE010000261         3740 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_15293_length_3740_cov_8.204613, whole genome shotgun sequence.
ACCESSION   DAOQJE010000261 DAOQJE010000000
VERSION     DAOQJE010000261.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3740)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3740)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3740
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15293_length_3740_cov_8.204613"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2890
                     /locus_tag="PLT08_08095"
     CDS             <1..2890
                     /locus_tag="PLT08_08095"
                     /inference="COORDINATES: protein motif:HMM:NF026288.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNE04464.1"
                     /translation="MKNITSFENEIERLAVTLVNEAFEGINEHFVLILDDFHILDGVQ
                     PIYEFLNRFIQLVDDNCHVIISSRVLTALADLPLMAAREQVRGLSFSDLAFQVNEIQA
                     LLQQNKNILLSDEDAEKMFKESEGWITGLQFGVYEKDRKSGVGLSDYLGQQVLDRQTP
                     QMREILLRTSLMDEFDSSMCEQVLGLFYESKQDWDFFLRSILSNNLFALPVGSDGSYL
                     RYHHLFRDYLRERIKKERPDEVRPILERLSHVYETIGEWEKAYAIIRTLGDVNLLVEL
                     IDLTSFNNLQDTMRLIENWIHDLPPSVQRNHPLIVSINGTIKLIKGDTQGGIRQLETA
                     IEVFQTEGNIPHLALSLIRRSYGYLYLGDYQSGIADLENVIRLVEDQDDLQQLHAEAL
                     HVMSSYMMRLGKNRSALKLIEKAMDIRIRLNGSKDIARTLADIGGIYQFLGKYDEAEK
                     NLNKALEIYSRDFNLIEEANLLNNLGFMFYQNGNYEKAAFSFEEGMLCAKRSRHVRLE
                     TLLSISLGDLYTRLNDSEIAEQNYRHAENLLEERDDQFLLFSLLIGQANLALLRRDLF
                     AAGQLIDSLGETVKKNSSHYDNGQFNLLLGKFDMFSGEFVGAVSKLEKAELCFLQDEL
                     TLDVISARIWLSAAYVANGHSTTAIQKIKETIGNKPQHVALIAASQSLKWLKPLQKDI
                     KAGREVRDLLGRAEKLADQLPDIRRELRRQARVVEVPVAQLVINGFGNSKVLRDGREL
                     SPSDWQTQSVRELFFFFLSQDVPLTKEQVASRFWQELDDPAKLKLRFKNDMYRLRRAV
                     GSEVVRFENNQYVFNRSLNYEYDVEAFESHLLRAHSAKETEEMIEFYTRAVDLFAGPY
                     LNDMYFDWVIADRERLDQMYLATLITLADLHQKQAELNQALIVCQRAIDYSPIYEAAY
                     CSTMQVYARMQNRTAVIQTYEACKSALKKHLSMPPSPETEALYKKLLS"
     gene            2983..3129
                     /locus_tag="PLT08_08100"
     CDS             2983..3129
                     /locus_tag="PLT08_08100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE04465.1"
                     /translation="MKTQFALFTHYTQKINRHHIQLAVTIVSLALLVLGIGAPIDGSG
                     PGPR"
     gene            3130..3672
                     /locus_tag="PLT08_08105"
     CDS             3130..3672
                     /locus_tag="PLT08_08105"
                     /inference="COORDINATES: protein motif:HMM:NF036410.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5317 domain-containing protein"
                     /protein_id="HNE04466.1"
                     /translation="MVLLVGIVFGLLVGLGMAARHQVRYEAPNFHHLWLVPLAFLLQW
                     LETSREFPIIASQILFLAFIYFNRSLPGMQVLMAGAFLNLVVMATNGGHMPISPETAS
                     RLFPQENLWDFPDGTLIGAKDVLLPPEQTRFEWLADRFLPPVWLPYQFAFSLGDVFIA
                     AGAFWLLAKPTSFAKGKQTS"
BASE COUNT         1091 a          735 c          830 g         1084 t
ORIGIN      
        1 catgaaaaac atcacatctt ttgaaaacga aatagaacgc ctggctgtaa cattagttaa
       61 tgaggcgttt gagggaataa atgagcattt cgttttaatt cttgatgatt ttcatatttt
      121 ggatggtgtg caaccgattt atgaatttct caatcgcttt attcaattgg ttgatgataa
      181 ctgccatgtc attatttctt cccgcgtgct cactgcactg gcagaccttc cgctcatggc
      241 agcacgtgaa caagtccgtg gtttgagctt ttcagacctt gcctttcagg tgaatgagat
      301 tcaagctttg ctacagcaaa acaagaatat tttgctttca gatgaagatg ctgagaaaat
      361 gttcaaagag tctgaagggt ggattactgg gctgcaattc ggtgtgtatg aaaaagatag
      421 aaaatctggc gtcggtttaa gtgattacct tgggcaacaa gtcttggatc gacaaacgcc
      481 acagatgcgc gagattttgt tgcgcacttc tcttatggat gagtttgatt catccatgtg
      541 tgaacaggtt cttggacttt tttatgagag caagcaagat tgggattttt tccttcgatc
      601 aattctgagc aacaatttat ttgcactgcc tgttggtagt gatggaagtt atctgcgcta
      661 tcatcattta ttccgcgatt atttgcgcga aagaataaag aaggaaagac cggatgaagt
      721 ccgcccaatt ttggaacgat taagtcatgt ctatgagact ataggagaat gggaaaaggc
      781 gtatgcaatc attcgcacct tgggagatgt aaatttgctg gtggagttaa ttgacctgac
      841 cagttttaat aatctacaag atacgatgcg tttgatcgaa aattggatcc atgacttacc
      901 gccttctgtt caacgaaacc atccgcttat tgtttctata aatgggacca taaaacttat
      961 taaaggagac actcaaggtg gaatacgtca attagaaaca gctattgaag tgtttcaaac
     1021 agaaggaaat attccacatc tggcactctc attaattcga cgttcatatg gctatcttta
     1081 tttaggtgac taccaatccg gtattgctga tttagaaaat gtaatccgct tggttgaaga
     1141 ccaagatgat ttgcaacaat tacatgcaga agctttgcat gtaatgagtt cgtatatgat
     1201 gcgattaggg aaaaatcgat cagcgttgaa gttgatcgaa aaagcaatgg atataaggat
     1261 tcgactgaat ggcagtaaag atattgcgag aacgttggct gatattggag ggatatacca
     1321 atttttagga aaatatgacg aggcggaaaa gaatcttaat aaggcactag aaatttatag
     1381 cagagacttt aacttaatcg aagaagctaa tctattaaat aacttaggtt ttatgtttta
     1441 tcaaaatggt aattatgaaa aagcagcttt ctcttttgag gaaggtatgc tttgtgctaa
     1501 acggagccgc catgttcgtt tagaaacttt gctttcgatc agtttaggtg acctttatac
     1561 aagactgaat gattcagaga ttgcggaaca aaactacaga cacgctgaaa atttattgga
     1621 agaacgcgac gaccaatttt tacttttttc tttacttata ggacaggcaa acctagcact
     1681 gttgagacga gacttgtttg ctgctggaca gttaattgat agtttgggag agactgtaaa
     1741 gaaaaactct tctcactatg acaatggaca attcaattta cttttaggta aattcgatat
     1801 gttttcagga gaatttgtag gagcagtaag taaactggaa aaggcagaac tttgtttttt
     1861 acaagacgag cttactctgg atgtgatttc tgccagaata tggctttctg cagcatatgt
     1921 tgcaaatggg cattccacaa ccgccattca aaaaataaaa gagactatag gaaataaacc
     1981 tcaacatgtc gctttgattg cggcaagcca aagtttgaag tggctaaagc cgcttcaaaa
     2041 ggatataaaa gctggacgtg aggtgagaga tttgctaggt cgtgctgaaa aacttgcaga
     2101 tcaattgcca gatattcgtc gtgaactccg tagacaggcg cgagtcgttg aagttccagt
     2161 agcacaactg gtcattaacg ggttcggaaa ctccaaagtt ttgcgtgatg gacgcgaatt
     2221 gagcccgtcg gattggcaaa cgcaatctgt gcgggagtta ttctttttct tcctttctca
     2281 agatgttcca cttacaaagg aacaagtagc ttcacggttt tggcaggaat tagatgaccc
     2341 tgctaaatta aagctaagat ttaaaaacga tatgtatcgc ttacgccgtg cagttggaag
     2401 tgaagttgtt cgtttcgaaa acaatcaata tgtatttaat cgtagtctca attacgaata
     2461 tgatgtcgaa gcttttgaat cacacttatt gcgggcacac tcggcaaaag agactgaaga
     2521 gatgatcgag ttttacacga gggctgttga cctttttgcg ggaccttacc tcaatgatat
     2581 gtattttgat tgggtaatcg cggaccgtga acgtttggat caaatgtatc tcgcaacttt
     2641 aataacccta gcagaccttc accaaaaaca agccgagcta aatcaggcac tgatagtgtg
     2701 ccaacgtgcc atcgactata gcccaatcta tgaggcggca tactgctcaa ccatgcaagt
     2761 ctatgcccga atgcaaaacc gcacagcagt aatccaaacc tacgaagcct gcaaatctgc
     2821 cttgaaaaaa cacctttcta tgccaccatc tcctgaaacc gaggcgttat acaaaaaact
     2881 actatcctaa aatagctaaa aacagctatt ttgaagcctg ttttgaagcc tgggggtgac
     2941 tatcttaagg gcatcgtttc cgagaaaaga aaggatgctc aaatgaaaac acaatttgct
     3001 ctctttaccc actataccca aaaaataaac cgtcaccaca ttcaactggc ggtcactata
     3061 gttagccttg ccttattggt gctaggcata ggcgcgccaa ttgatggttc aggaccaggt
     3121 ccgcgctaaa tggttctttt ggtaggaatc gttttcgggt tgttagttgg cttaggaatg
     3181 gctgcccggc atcaggtccg gtatgaggcg ccgaattttc atcacttgtg gctggtgccg
     3241 cttgccttcc tactccaatg gctggaaaca tcccgagaat ttcccatcat tgcttcacag
     3301 attttgtttt tggcgttcat ctatttcaac cgcagtctgc cgggaatgca ggtcttgatg
     3361 gcaggcgcgt tcttaaatct tgtggtgatg gcaacaaatg gcgggcatat gcccattagc
     3421 cctgaaactg ccagcaggct tttcccgcaa gaaaatcttt gggatttccc cgacggaact
     3481 ttgatcggcg ccaaagacgt tttattgccg ccggaacaaa ctcgcttcga atggctcgcg
     3541 gatcgtttcc tccctcctgt ttggttgcca taccaatttg cctttagcct cggtgacgtt
     3601 tttatcgcgg cgggtgcatt ttggttgctt gccaaaccga cttcttttgc caaaggaaaa
     3661 caaacttcat gatcgccact tccttcaacc gtaattcaga gaaccatcat acaaaacctc
     3721 agcagcgtat atctaaggca
//
LOCUS       DAOQJE010000262         3721 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_15439_length_3721_cov_5.999182, whole genome shotgun sequence.
ACCESSION   DAOQJE010000262 DAOQJE010000000
VERSION     DAOQJE010000262.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3721)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3721)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3721
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15439_length_3721_cov_5.999182"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..733)
                     /locus_tag="PLT08_08110"
     CDS             complement(<1..733)
                     /locus_tag="PLT08_08110"
                     /inference="COORDINATES: protein motif:HMM:NF014763.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cache domain-containing protein"
                     /protein_id="HNE04467.1"
                     /translation="MKKIFSSNRSSTPLSASLQSVSLQTRLLLTSIFLVLIPALITTL
                     TSTYISSRGLTTDSFDQLESVATLKENQVRTWLNTLQTSLELVFSDQASVQRVANVIN
                     AEEDNDVFIEKAKLRFDLGRYNGLSGYFTEIFIMNTDGVVVLSTVKDQEGKIFSNQTF
                     FSEGIKGPYINPPIFEPSFSNYSIIISQPLVSQNGKALGVLAGRVNLSTLNDIMSERA
                     GLGETGETYLVNANFAVLTNLRFQET"
     gene            complement(854..1846)
                     /locus_tag="PLT08_08115"
     CDS             complement(854..1846)
                     /locus_tag="PLT08_08115"
                     /inference="COORDINATES: protein motif:HMM:NF020650.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HNE04468.1"
                     /translation="MKIYSRLFLVFAITALLSACGAFKSAAPADKVTVQLSWFHGVEY
                     SGFYAAIDQGYYKDANIEVTLNAGGPDINQFDEVSNGNAQFGIGSGDSLITARANGQQ
                     FVAVSTIFKENPLAITSLTKDNITKPDDLVGKTVGVYSTDLSNFNDLPFLALMSRTNL
                     DRESMKYALIQDFQGANEIKAGNMDAMSGMFATDQQVMASQAGDELNFIYYKDYGYDV
                     YINAIFVTDEFRQNNSDLIRRFVQATIKGYQYAIENTDAAAQLALTYDETLDPAYQQA
                     VMQAQIPFINSGDGQIGVMNENIWENTQNILLEFGLISSPIDLSTVYTNEFATP"
     gene            complement(1860..>3721)
                     /locus_tag="PLT08_08120"
     CDS             complement(1860..>3721)
                     /locus_tag="PLT08_08120"
                     /inference="COORDINATES: protein motif:HMM:NF024883.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNE04469.1"
                     /translation="VTLLTRYLEYEGYDQVVARDGLEAINKIRDEMPDLILLDVNMPN
                     KDGFTVLSEVRADSAIAHIPVIILTAARLDPSDVQSGLNLGADDYVTKPFDRHELMAR
                     IRTKLRVKEAEDITRRRNRELNLLPEIGKQLSARLDPKDLANVLLKRTAETLGAIQAN
                     MYILDSEEKEIQEKYEVVLNPSGAAENLTPPAKLIQHVQENHQGVVIEDAVNDPLWQV
                     DAQTHIRSALVVPIFGRYALLGLIILTHEQESYFSSDHLLLLQAIASQAAIAIENTRL
                     YSVVLQEQKKVAAILRHASEAILLFDEQGNLTLLNPAGEQLFLMHPMKMNQPLPTDYG
                     YDGLIKLIEDARTSQMSISGEIVWVDQRTFTAFVAPIEDGGTVAILHDVSRFKDLDQV
                     KNEFIATASHDLKNPLTSIGGFAQLLLQAGPLNENQKEFVDRIQHSVKSMSELIQNMM
                     ALAQADLNATQKHEPVDIKEVLDEVVNEFAPQARGKEQNLEFTSPTSHVQVNGDSLHL
                     RQAFRNLLGNAIKYTPKGGLIQVQSVIEQGNLLVHFKDNGYGIPATDLPFIFNRFYRV
                     RTGKASEVEGNGLGLAIVKAITVEHNGSVNVESQAGKGSCFSITLPLLNNNFA"
BASE COUNT         1026 a          805 c          830 g         1060 t
ORIGIN      
        1 tggtttcttg gaaacgtaag ttggtcaata cagcaaaatt ggcattaacc aaataagttt
       61 cgccggtttc tcccaagcct gcacgttcgc tcatgatgtc gtttaatgtg gaaagattaa
      121 cacgccctgc taaaacccca agcgcttttc cgttttggct caccaaaggt tgtgaaataa
      181 taatggaata attcgagaat gaaggttcaa atatgggagg gttgatatac ggtcccttaa
      241 taccttcaga gaaaaaggtt tggttgctaa atatcttgcc ttcttgatct ttgacggtgg
      301 agagtacaac cacaccatct gtgttcataa tgaagatttc agtaaagtat ccagagagac
      361 cgttatagcg ccctaaatca aagcgtagtt tggctttttc tataaaaaca tcgttgtctt
      421 cttcagcatt gatcacattc gccactcgtt gaaccgatgc ctgatctgaa aataccaatt
      481 ccaagctggt ctgcagggta ttcaaccatg tccgaacctg attctctttg agtgtggcaa
      541 cagactctaa ttggtcaaaa gagtcggttg tcagaccgcg agaactgatg taggttgatg
      601 tcaatgttgt gattaaagct ggaataagca ccaggaaaat gcttgttaag agcaaacgcg
      661 tttgcagcga aactgattgt aacgatgcac ttaatggggt gctacttcga tttgaagaga
      721 aaattttctt catgaaactc tccggacaca aaattgaatg aaatctggtg tcttatcttt
      781 tttataaata ggtaatgcgt aaagtcaagt gatattgaag ttactattca atatcacttg
      841 acttgaaaaa actttatggg gtcgcgaact cgttcgtgta taccgttgat agatcgatgg
      901 gggatgaaat caacccgaat tcaaggagaa tgttctgggt gttttcccaa atgttttcat
      961 tcataacgcc gatttgccca tcaccagagt tgatgaaagg aatctgggct tgcataacag
     1021 cttgttgata agcaggatcc agagtttcgt cataggttaa tgccagttgg gccgctgcat
     1081 cggtgttttc aattgcatac tgatagccct taatggtagc ttgaacaaaa cgacgtatca
     1141 gatcagagtt attctgtcta aattcatctg tgacgaaaat agcgttgata taaacatcat
     1201 aaccatagtc tttgtaatag atgaaattta attcatcacc tgcttggctt gccatcactt
     1261 gttggtctgt agcaaacata ccactcatag catccatgtt ccctgctttg atttcatttg
     1321 caccttgaaa atcctgaatc aaggcatatt tcatggactc acggtcaagg ttggtgcggc
     1381 tcatgagagc aaggaaaggc aggtcgttaa aattgctaag atcagttgaa taaacaccga
     1441 ctgttttccc gaccaagtca tcaggcttcg taatgttatc ctttgttaag gatgtgattg
     1501 caagagggtt ttccttgaaa atggtggata ccgctacaaa ttgttgccca ttggcgcgag
     1561 ccgtgatcag gctgtcacca cttccaatgc cgaattgggc atttccgttg ctaacttcat
     1621 cgaactgatt aatatccggc ccaccagcat tgagtgtgac ctcaatattg gcatctttgt
     1681 agtatccctg atcgatggca gcataaaaac cagaatattc cacgccatgg aaccagctca
     1741 attgaacggt caccttatct gccggagccg ctgatttaaa tgcgccacaa gcgctcagta
     1801 gtgcggtgat tgcaaaaact aaaaataaac gagaatagat tttcattggg ttcctctttc
     1861 taggcaaaat tattgttcaa caacggcaag gtgatgctaa aacatgaccc cttcccggct
     1921 tgactttcta catttactga accattatgt tcaactgtga tggcttttac gatggcaagc
     1981 cccaacccat tgccttccac ttcgctggct ttcccagtgc gaacgcgata aaaacgattg
     2041 aaaataaaag gcaggtcagt agcagggatg ccatatccat tgtctttgaa atgaacgagc
     2101 aggttccctt gttcgattac agactggacc tgaataagcc cgcctttagg cgtgtactta
     2161 atggcgtttc ccagtaaatt ccggaatgct tggcgtaaat ggagcgagtc accatttacc
     2221 tgcacatgag aggtgggtga ggtgaattca agattttgtt ctttgccgcg cgcttgtgga
     2281 gcgaactcat taaccacttc atccaaaact tctttaatgt caactggttc gtgcttttgg
     2341 gtagcgttca aatctgcctg agccaatgcc atcatatttt ggattaattc gctcatgctc
     2401 ttaacggagt gttgaatgcg gtctacaaac tctttttggt tttcgttaag tggacccgcc
     2461 tgaagcagca attgcgcaaa cccaccaatc gaagtaagcg gattcttcaa gtcatgtgag
     2521 gcggtggcga tgaattcatt cttgacctga tccaagtctt tgaagcggct cacatcgtgc
     2581 aagatggcaa cggtcccgcc atcttcaatt ggagcgacaa aggcagtgaa tgtgcgttgg
     2641 tcaacccaaa cgatttcgcc cgaaatagac atttgagatg tacgtgcatc ttcaattaac
     2701 ttaataagcc cgtcgtatcc ataatctgta ggcaaaggtt gattcatttt catggggtgc
     2761 atcagaaaaa gctgttcgcc tgcagggttt agcaaggtta ggttgccttg ctcatcgaag
     2821 agcaaaatcg cctctgaggc gtgtcttaaa atcgcagcaa cttttttctg ctcttgcaat
     2881 accacggaat acaatctcgt gttttcaatg gcaatggcag cctggcttgc aatggcttgt
     2941 aataacaaca aatggtcgct tgaaaaataa ctttcctgtt cgtgagtcag aatgatcaac
     3001 cccaataatg cataacgacc aaagatgggc acaaccaaag ccgaacggat gtgggtttga
     3061 gcatccactt gccagagcgg gtcgttgacc gcatcttcga ttaccacccc ttgatgattt
     3121 tcctgtacgt gttgaataag tttggcaggc ggagtaaggt tttcagcagc ccctgaagga
     3181 ttcaaaacca cttcatactt ttcttgaatc tctttttctt cagagtctaa aatatacata
     3241 ttggcttgaa tagcacccaa agtttcggcg gtgcgcttga gcaaaacatt tgcaaggtct
     3301 ttggggtcta aacgagcgct gagttgtttg ccaatttcag gcaataaatt taactcgcgg
     3361 ttgcggcgac gcgtaatatc ttcggcttct ttcacacgta acttggtgcg aatgcgcgcc
     3421 attagctcat gacggtcgaa aggcttggta acgtaatcat cggcaccaag gttcaagcca
     3481 gattgcacat cagagggatc taaacgagcg gcggtcaaaa taatgaccgg aatatgagca
     3541 atcgccgaat ctgcgcggac ctctgaaagg acagtgaagc cgtctttgtt cggcatatta
     3601 acatccaata ggatgaggtc tggcatttca tcacgtatct tgtttatcgc ttccagcccg
     3661 tcacgcgcaa caacctggtc atacccctca tattcaagat aacgggttaa cagagtgacg
     3721 t
//
LOCUS       DAOQJE010000263         3718 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_15469_length_3718_cov_3.183183, whole genome shotgun sequence.
ACCESSION   DAOQJE010000263 DAOQJE010000000
VERSION     DAOQJE010000263.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3718)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3718)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3718
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15469_length_3718_cov_3.183183"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..575
                     /locus_tag="PLT08_08125"
     CDS             <1..575
                     /locus_tag="PLT08_08125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE04470.1"
                     /translation="SYDPITGSVRQPLWRMIIAVPIFYGALSYLIASFPQKILIWQNL
                     GFILAILSGGLTFWMNYRPSQIAKSLVFTSLASMFMLISIRSFSFLFPNSKVVWILLI
                     VLSFVMTYLPQLFQQPSLLKSEFFQKGNYYLPFILPAIGLVGTIFGLITTRANMLHVQ
                     AVFLGLIAWLLGIALSFSLSPPQMNENDAD"
     gene            820..1554
                     /locus_tag="PLT08_08130"
     CDS             820..1554
                     /locus_tag="PLT08_08130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HNE04471.1"
                     /translation="MNNLFEKISTYNPFQLTFLQKIVENVEKRLSTQSLNTTELQSLI
                     TDITANEICQNDQLIELKEVRDTLISFLQEKGDVVHYLISSLHEYQSRYFAASFLGWF
                     GNSAKVALPELIDLASGHGIASGAARKSILLIGDAESEMLNAIMESVRFEDDESFRNL
                     TTLATKTKLATSNSFFEMLKSCSKHSNPYFRELVADVIWSLGSLDQEEIISILKILSS
                     DKEEFVRDAALEALNMISSGRNKYSG"
     gene            complement(1912..2678)
                     /locus_tag="PLT08_08135"
                     /pseudo
     CDS             complement(1912..2678)
                     /locus_tag="PLT08_08135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012251879.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5 family transposase"
     gene            2754..3062
                     /locus_tag="PLT08_08140"
     CDS             2754..3062
                     /locus_tag="PLT08_08140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE04472.1"
                     /translation="MKRATAEIELIQKYITTDVTPPLEEASRNQTQYVRQYQDLEQVY
                     ASLNQIQQRLAILKDAVNPVPNSEITSIPPTLQRQLSHVRDSFFEDIAKQQNEPHKDR
                     "
     gene            3070..>3718
                     /locus_tag="PLT08_08145"
     CDS             3070..>3718
                     /locus_tag="PLT08_08145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE04473.1"
                     /translation="MNIKTSRLPNEVENYRNRMRTGYWDIKMPEIVTRYWRNEIEPSN
                     LYGLGEEGVKIFLKKHKNLSEKNLICFARQAETYGYIEMENGFWKHAYLRSTKNSRVS
                     KSKRRESKTSQSINSEKQNFALSFQELWFTILREQEKYSCYSIFFALPSDKEAMRYLE
                     EYNNDLQLISSNTMVIKMGRDKLLHVEIGGKSLSAEVDYGRLAHLFEINFTMFPCV"
BASE COUNT         1114 a          733 c          762 g         1109 t
ORIGIN      
        1 caagttatga ccctataact ggttcagttc gacagcccct ttggaggatg atcattgcgg
       61 tacctatttt ctatggggct ctcagctact tgatcgcttc attccctcaa aagatcttga
      121 tatggcaaaa cctaggcttt atattggcca tcttgagcgg cggattgaca ttttggatga
      181 attatcgtcc cagccaaatt gctaaatcac ttgtgtttac gagtttggca tccatgttta
      241 tgttgatctc cattagatct ttcagctttc ttttcccaaa ttcgaaggtt gtatggatat
      301 tgctaatcgt gttaagtttt gtgatgacat atttacctca gttgtttcag cagccttctc
      361 tccttaagag tgagttcttt caaaaaggta attactattt accttttata ttaccagcaa
      421 ttggacttgt gggaacaatt tttggtttaa ttacaacaag agctaatatg ttgcatgttc
      481 aagctgtttt cttaggtttg attgcatggc ttcttggtat tgcattgtcg ttttctttat
      541 ctccgccaca gatgaatgaa aatgatgcag attagacaat aattcatccc ccgccacaca
      601 ggagcctcga acggctctgg ctagaactcg atcttcaaat gcctcggctc caccagcatt
      661 gtgaccaacg ccagcggaaa caaggtctcg gagatgagat agtataaatt ccggttagga
      721 ggagacaatg tctacgtatg atttttatca aattgtattt tcttgttttt taacatcgat
      781 gggaggatgt tacttaggat ttaaaggata tctgatgata tgaataatct ttttgagaaa
      841 atttctacct acaatccatt tcaacttacc tttttacaaa agatcgtaga aaatgttgaa
      901 aaaaggcttt cgacgcagtc tttgaacaca actgaattgc aaagccttat aaccgatata
      961 actgcaaacg agatatgcca aaatgatcag ttaatcgaat tgaaagaggt gcgtgataca
     1021 ttaataagct ttttacaaga aaaaggagat gtagttcact atctgattag ctctctgcac
     1081 gaatatcaat caagatattt tgcagcgagt tttttaggtt ggtttggaaa tagcgcaaaa
     1141 gttgcattgc ccgagctaat tgatttggca agtggtcatg gtattgcttc aggcgctgct
     1201 cgaaagtcta ttcttcttat cggtgacgcg gaatccgaga tgctgaatgc gattatggaa
     1261 tctgtaagat ttgaagatga cgagagcttt aggaacttaa ccacgttggc aaccaaaact
     1321 aaacttgcta catccaatag tttttttgaa atgctgaaat cttgttcaaa gcatagcaac
     1381 ccctatttcc gagaattggt cgctgatgtt atatggtctc ttggaagctt ggatcaggaa
     1441 gagataatct ctattctaaa aatattatct tctgataaag aagagtttgt cagagatgct
     1501 gcattagaag cactcaatat gatatcgtca ggaagaaata aatattcagg ttaacaataa
     1561 tcgtcttaca atttttttga aatacgtgta agaactatgt tcaaaattct attaaaggcg
     1621 taacctcacc aagttcgttt ttgataaata cgggttcagc aatgcttgga atgcatacga
     1681 ttatgggtgg ggagagcatg ctggtgaacc aatggaagat ttttaggcat ctgttgcagt
     1741 agatgtagct ataactatag ttgttccagt tcttacagca atagttatag ggactgtaat
     1801 gctggctata ggagtagtat ctcttccggc ggtggctttt gttggtggtg tcgcgctaat
     1861 ttcaacaagt gtatctatgc tgcttgaacg gaatagctag aactcatctc aaaaatagtt
     1921 tctgataaaa acaacaatgc aagccagatg aacaaaagcg aggtaattct caagaaatcg
     1981 atcgtagcga accagaatgc gacggtagtt ttgtaaccaa gcaaacaagc gctccacctt
     2041 ccatcgcctg cgatagcgac gtaactcgcg accatcttgg gtctttggct tctggcgatt
     2101 ggatttgtgc ggcgcaatca acaagatgcc ctgtttggct aacttctcat ccaaaggatc
     2161 actatcgtac gctcgatctc ctatcaatcg ttcaggattc tcgtccacta atcggtcatc
     2221 caaggtcttc tcgactagct tgacttcatg cggtgaagca cttccaacgg atatggcgac
     2281 aggaaaacca gcgccatctg ccactgccat gagctttgta cctttgcccc gcttggtttt
     2341 tcccacccct gagccccttt tttagccatg acaaaggtgc catcgatgaa acattctgtc
     2401 agatctaagt tgcctcgttc tttcacatcc tgtaccagtt tcctgaggat agtctcaaat
     2461 gtacctgttt ggacccattc ctggaaacgt cgatggcagg tttggtacgg tggataccga
     2521 ttgggcatat catgccaagg agcacctgtt cgcatgaccc aaaggatgcc attcaagatg
     2581 gcgcggttat ccactcgtgg tctgcctctt ccatctgccc gtttcacggg tttaggtaat
     2641 agaggttcta ccactgccca ttgttcgtct gtaagatcca ttcgctcgct cccaacttgg
     2701 gtttgagcgc cagtttacca tttttgagat aacttctaga aatttcccat atattgaaac
     2761 gcgctaccgc agagatcgaa ttaattcaaa aatacattac tacagatgta acacctcctc
     2821 tagaagaagc aagccgcaat cagacacaat atgttaggca atatcaagat ctcgaacagg
     2881 tgtacgcaag tctaaatcaa attcaacaac gtcttgctat attaaaagat gctgtaaatc
     2941 ctgtgcctaa ttcagaaatt acctccatac caccgacttt gcagcgtcaa ctaagccatg
     3001 ttcgagatag cttttttgaa gatatagcta agcagcaaaa tgagcctcac aaagacaggt
     3061 aggtaaaaaa tgaatataaa aacttcaaga cttcccaacg aagtggaaaa ctaccgaaat
     3121 agaatgagaa caggttattg ggatataaaa atgcctgaga ttgttacgcg gtattggcga
     3181 aacgagattg aaccttcaaa cctttatggt ttaggagaag aaggcgttaa aatattcttg
     3241 aaaaagcata aaaacctaag cgagaagaac ttaatatgct ttgcgcgtca agcagaaacc
     3301 tatgggtata tagagatgga aaatggcttc tggaaacatg cttatctaag atctacaaaa
     3361 aattcacgtg tctctaaaag caagagaaga gaaagcaaaa cctcgcaatc gataaacagt
     3421 gaaaaacaaa atttcgcctt aagctttcaa gaattatggt ttactatcct acgggaacag
     3481 gaaaaatatt cttgttactc aatcttcttt gcacttccat ccgataagga ggcaatgcgt
     3541 tatttggaag agtacaataa tgatttacaa ttaatttctt caaatactat ggttataaaa
     3601 atgggacgcg ataagctttt acacgttgag ataggcggga aaagtttgtc agcagaagtg
     3661 gactatggaa gacttgctca cttatttgaa ataaatttta caatgttccc atgcgttg
//
LOCUS       DAOQJE010000264         3715 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_15487_length_3715_cov_7.193989, whole genome shotgun sequence.
ACCESSION   DAOQJE010000264 DAOQJE010000000
VERSION     DAOQJE010000264.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3715)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3715)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3715
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15487_length_3715_cov_7.193989"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            47..769
                     /locus_tag="PLT08_08150"
     CDS             47..769
                     /locus_tag="PLT08_08150"
                     /inference="COORDINATES: protein motif:HMM:NF014566.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II CAAX endopeptidase family protein"
                     /protein_id="HNE04474.1"
                     /translation="MDQYPLDNNPNLTPPLSTPKPASWSANDTWLGLALLLIVVAGYL
                     FALSRMEQNQSTNILYVATFEFLLLIPIAVIFLWRKVSWKELGFNRFNANQLALGCGL
                     LVGVYMLVIINNLVMISLGVTTQADVISELLGEINAPYLFAFTTVIVAPITEEMFFRG
                     FLFKGLREKYGWVNALMFSSMIFAIFHGQLATLLPTFLLGALFSYLYQRTDSVFPGMF
                     LHFTVNAMGALVLLFANQTGAL"
     gene            complement(808..2058)
                     /locus_tag="PLT08_08155"
     CDS             complement(808..2058)
                     /locus_tag="PLT08_08155"
                     /EC_number="1.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HNE04475.1"
                     /translation="MNTKQKPHVLIIGAGFAGLETARGLAKAPVRITLIDKNNHHLFQ
                     PLLYQVAIAGLLPSQIAQPVRTIFRKQKNLAFQMGEVTDINLNEKFVKMNGSVIAYDY
                     LVIAAGARTNFFGFDTLEKHGLQLKDLETAVTTRNHLLSRFEGASHEADPEKRKAMLT
                     FVIVGGGPTGVETAGALAELITHVMRKDYPRLNLNEAQVILLEAGPNLIAPYPDELRQ
                     ATLRLLRDKKVDVRLNTKMQDFNGQRVTLGDGSVIESQTLIWTAGAKAAEVIDALPAE
                     KASMGRVRIQATLSLPQFPEAFVIGDASFLANGNGQPLPMLSTVAIQQGQAAAKNIRL
                     MMEGKQPKAFYYKDPGLLATVGRNAAVARIFGISFSGFIAWVIWVFLHIYRIIGFRNR
                     LIVMFNWAWDYFFYDNQVRLITRE"
     gene            complement(2234..3307)
                     /gene="arsB"
                     /locus_tag="PLT08_08160"
     CDS             complement(2234..3307)
                     /gene="arsB"
                     /locus_tag="PLT08_08160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008490 - arsenite secondary active
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACR3 family arsenite efflux transporter"
                     /protein_id="HNE04476.1"
                     /translation="MTNEKRKELSFLDRYLTLWIFLAMAVGVGIGFLFPKTVADFNMA
                     VSVGTTNIPIAIGLILMMYPPLAKVHYDELGDVFRNWKILSLSLIQNWVIGPILMFVL
                     AIIFLRGYPEYMTGLILIGLARCIAMVIVWNELAKGDSEYAAGLVAFNSIFQVLFYSV
                     YAYVFITVLPTWFGMTGNIVDITIGEIAKSVFIYLGIPFLAGFFTRFFLVRAKGKDWY
                     HKVFVPKISPMTLIALLFTIVVMFSLKGDVIVQLPLDVVRIAIPLLIYFVVMFLVSFF
                     MGKSIGADYSKTTTLSFTAASNNFELAIAVAVAVFGIGSGAAFAAVIGPLIEVPVMIG
                     LVNVAFFFQKRYFTSTVQHAPAR"
     gene            complement(3320..3715)
                     /locus_tag="PLT08_08165"
     CDS             complement(3320..3715)
                     /locus_tag="PLT08_08165"
                     /EC_number="1.20.4.4"
                     /inference="COORDINATES: protein motif:HMM:NF013607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030612 - arsenate reductase (thioredoxin)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase ArsC"
                     /protein_id="HNE04477.1"
                     /translation="MKKVLFLCTGNSCRSQMAEAIVNARMGAEWYAVSAGTKPAGYIH
                     PKALTVLSEIGIQHEGRSKRADEFQDESFDLVVTVCDSAAEECPVWLGKGKKVHHSFP
                     DPALTDDMNDFRAVRDDIEKVILELLKTN"
BASE COUNT          938 a          975 c          874 g          928 t
ORIGIN      
        1 gaccgtaaag gtcaatggct gatgtataga gaattggaaa aaacaaatgg atcaataccc
       61 gctggacaat aacccgaacc tcacgccgcc tctttccacg cccaagcctg cctcttggtc
      121 tgcaaacgac acctggctcg ggctggctct tctgctcatc gtcgttgccg ggtatttatt
      181 tgcactttct cgcatggaac aaaatcaaag caccaacatc ttgtatgttg ccaccttcga
      241 attcctttta ttgattccca ttgccgttat cttcttatgg cgcaaagtct catggaaaga
      301 gctgggcttc aaccgtttca atgccaatca attggcattg ggttgcgggt tgttagttgg
      361 cgtgtatatg ctggtcatca tcaacaatct cgtgatgatc tcactcggcg tcaccaccca
      421 agccgatgtc atctccgagc ttcttggcga aattaacgcc ccgtatctct ttgcctttac
      481 caccgtcatt gtcgcgccta tcaccgaaga gatgttcttc cgtggctttc tcttcaaagg
      541 cttgcgcgaa aaatatggct gggtcaatgc cttgatgttc agctcgatga tctttgccat
      601 ttttcatggg caacttgcta ctttattgcc tacttttctg ctcggcgcct tgttctctta
      661 cctgtaccag cgtacagact cagtcttccc gggcatgttt ctacatttca cggtcaacgc
      721 catgggggca ttggtcttgc tcttcgcaaa tcaaaccggt gctttataag aatacatcct
      781 cagagaaaat ctctgaggat gtattcttta ttcccgcgtg attagtcgta cttgattatc
      841 gtagaagaaa taatcccacg cccaattgaa catcacaatc agacggttgc gaaacccgat
      901 aatgcgatag atgtgcagga agacccagat cacccaggca atgaatccac tgaaggagat
      961 gccaaagatg cgggcgacgg cggcgttccg tcccaccgtg gcgagcagac cggggtcttt
     1021 atagtagaac gctttgggtt gcttcccttc catcatgaga cgaatattct tcgccgcggc
     1081 ttgaccttgc tggatcgcca cggtcgaaag catgggcaaa ggttgaccat ttccattggc
     1141 gagaaaagaa gcatcaccga tcacaaaggc ttcaggaaac tgaggcaggc tcaacgttgc
     1201 ctggatgcga acgcgaccca tgctggcttt ctcggctggt aacgcgtcaa tgacctcggc
     1261 agctttcgcg cccgcagtcc aaattagcgt ttgactttcg atcacagagc cgtctcccag
     1321 cgtcactcgt tgaccgttga aatcctgcat cttggtgttc aagcgcacat ccactttttt
     1381 atctcgcaaa agccgcaacg ttgcctgacg cagttcatcg ggatatggcg cgatcaaatt
     1441 cggacctgcc tccagtaaga tgacttgagc ctcgttcaaa ttcaaacgcg gataatcctt
     1501 gcgcatcaca tgtgtgatga gctctgctaa tgcgcctgcc gtttccacgc cggtgggacc
     1561 tcccccaacg atgacgaagg tgagcatggc tttacgtttt tcggggtcgg cttcgtggct
     1621 ggcaccctcg aaccttgaaa gcagatgatt gcgtgtggtg acggcggttt caaggtcttt
     1681 gagttgcaag ccgtgttttt cgagcgtatc aaagccgaag aagtttgtgc gcgctcccgc
     1741 cgcaatgaca agataatcat aggcgatcac ggagccgttc atcttcacga acttttcgtt
     1801 caaattgata tctgtcactt cgcccatttg gaaggcgagg ttcttttgtt tgcggaatat
     1861 cgtgcgcacg ggttgggcaa tttgcgatgg caacaaacca gcaatcgcca cctgatacaa
     1921 cagcggctga aagagatgat ggttgttctt gtcgataagg gtgatgcgta caggcgcttt
     1981 ggcgagtccg cgggcggttt cgagcccggc aaagccagcg ccgataatta agacatgggg
     2041 cttttgcttg gtgttcatgg tttcctctgt gttgttatgc gattcatttc taattgtgaa
     2101 atttatcacc attattataa ccaaaactgt cttatttgta aatattttca caataggatg
     2161 ttagtactgc cccacttaac ggaaaagcct cccgcttcat ttcaacgaga ggcttgatac
     2221 gaccagtgag tttttatctg gctggggcgt gttgcacagt cgacgtgaag taccgcttct
     2281 ggaagaagaa cgccacattg accaacccga tcatcaccgg cacctcgatc aacggaccga
     2341 tgaccgccgc gaatgccgca ccagaaccga tgccaaacac tgctacagcc acagcaattg
     2401 ccaactcaaa gttattgcta gcagccgtga atgaaagcgt ggtcgtcttg gaatagtccg
     2461 cgccgatgga tttgcccata aagaatgaga caaggaacat caccacaaag taaatcaaaa
     2521 gcggaatagc aatacgcacc acatcaagcg gcaattgaac aatgacatcc cccttgagcg
     2581 agaacatcac aactatagtg aagagcaatg cgatgagagt cattgggctg atcttcggca
     2641 caaagacctt gtgataccaa tccttgccct tcgcacgtac aaggaagaag cgcgtgaaga
     2701 aaccggcgag gaacgggatg ccgaggtaaa tgaacacgct cttcgcgatc tctccgatgg
     2761 tgatatccac aatattgcca gtcatgccga accaagttgg caagacggta ataaaaacat
     2821 aggcatatac actgtagaaa agcacttgga agatgctatt gaaagcgacc aatcccgccg
     2881 cgtattcgct atcgcctttg gcaagttcgt tccacacgat gaccatcgcg atacaacgcg
     2941 caagtccgat caaaatcaag cccgtcatat attccggata gccgcgcaag aagatgatcg
     3001 caagaacgaa catcaagatc ggaccgatca cccagttttg aatgagcgaa aggctgagga
     3061 tcttccaatt gcggaagaca tcgcccaact catcgtagtg gacctttgcc agcggcggat
     3121 acatcatcag gatcaatccg atggcaatcg gaatattcgt cgttcctacc gaaacagcca
     3181 tgttgaaatc tgccaccgtt ttggggaaga gaaagccaat ccccaccccc acagccatgg
     3241 caaggaaaat ccacagggtt agatatcggt cgagaaatga aagttccttg cgcttttcgt
     3301 ttgtcatttt gcctccgtat taattcgttt ttagcaactc cagaatcact ttttcaatat
     3361 catcgcgcac agcgcgaaag tcattcatat catcggtcaa cgctgggtcg gggaaactat
     3421 gatgaacctt cttccccttc cccaaccaga cgggacattc ctctgccgct gaatcacaaa
     3481 cagtgacgac gaggtcaaag ctttcgtctt gaaattcgtc tgctcgcttc gacctaccct
     3541 catgctggat cccaatttca gaaagcacag tcaacgcctt ggggtggata taacccgcag
     3601 gcttcgtccc cgcgctgacg gcataccact ctgcgcccat ccgcgcattg acgatggctt
     3661 ctgccatttg cgaacggcag gaatttccgg tgcagaggaa taaaactttt ttcat
//
LOCUS       DAOQJE010000265         3703 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_15565_length_3703_cov_6.141447, whole genome shotgun sequence.
ACCESSION   DAOQJE010000265 DAOQJE010000000
VERSION     DAOQJE010000265.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3703)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3703)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3703
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15565_length_3703_cov_6.141447"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..284
                     /locus_tag="PLT08_08170"
     CDS             <1..284
                     /locus_tag="PLT08_08170"
                     /inference="COORDINATES: protein motif:HMM:NF017050.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="GMC family oxidoreductase"
                     /protein_id="HNE04478.1"
                     /translation="NEAARTYAGISDGMPHNSVLESMFNMSVTAHILGGCPIGVDSQS
                     GVIDSRHEVFGYPGLYVMDGSAIPANVGVNPSLTITALTERAMSLFPQK"
     gene            complement(305..2680)
                     /locus_tag="PLT08_08175"
     CDS             complement(305..2680)
                     /locus_tag="PLT08_08175"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:NF024703.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HNE04479.1"
                     /translation="MDTNPNASLEADHTHSISVRSLVEFVMQSGDLTAGGFQRRDRAQ
                     LGTLGHKQVQKSRPEGYDTEVEIICRIENTNPPLEIRGRIDGLYAGKEPVIIEEIKTT
                     TLSLDLIHDDHNPLHWGQAQCYAYMYALQQNLSEINVHLTYYQLDSHEEKTFERHFTW
                     AELESFFSNLITQYLDWFRKVQAWQARRDRSIEQLEFPYDEYRPGQRDLAVTVYKTIR
                     ANDRAYIQSPTGVGKTIATLFSSVKALGQGLATKIFYLTAKTSGRLVAEKALEDMYRS
                     NLYLRSVTLTAKEKICFCPPVNCDPEVCIFARGYFDKVKLALQELDQHQTFTRPVIEE
                     LARKYEICPFEFSLDVALWVDCIICDYNYVFDPRVYLHRFFDFTTEPYIFLVDEAHNL
                     PDRARAMYSAELDKETVLSLQRTLKPHVPALAKKLGEVNKILLEKRKACEAEDKDALI
                     EHDLPETLLKTLREFSHKAEDWLVMNQVAEFRQELLEFYFLCSNFLRTAEYFDTYYVS
                     YFERRGQSDLRAKLFCLDPAPLLAFPLERSCATIFFSATLLPMNYFKKLLTGATDHIS
                     RTFESPFPQENACLLIHNKISTKYTQRADSYDDIAELIETIIKTHTGNYLVYFPSYVY
                     LNEVVERLKQRLPDRQLLIQERGMDENARDSFLAQFSTTNEETLVGFAVMGGVFGEGI
                     DLVGDRLIGVIVVGVGVPQLGLERDLIKEYFDRDLGRGYAYAYQYPGFNRVLQATGRV
                     IRTDTDRGIIVLIDERFTQTQYRQLFPSHWRGYQVVKNIHEVEDKLTHFWK"
     gene            2800..>3703
                     /locus_tag="PLT08_08180"
     CDS             2800..>3703
                     /locus_tag="PLT08_08180"
                     /inference="COORDINATES: protein motif:HMM:NF015168.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF255 domain-containing protein"
                     /protein_id="HNE04480.1"
                     /translation="MPNYLISASSPYLLQHANNPVDWYPWGEEALNKAKAENKPIFLS
                     IGYAACHWCHVMAHESFEDSETAAFMNEHFINIKVDREERPDLDNIYMQATIAMTGSG
                     GWPMSVFLTPELKPFYAGTYFPPVRRYNMPSFREVLEGLSNAWVNDREEIEKTSNKVF
                     EHLDTKLKSNNEENLTTDHLEAIAKAMQGAYYWGTGGWGDAPKFPQPMALEFLLHHAV
                     TTKQNEYIKLIEHCLKVMARGGMYDVVGGGFSRYSTDDHWRVPHFEKMLYDNAQLVRV
                     YLHAWQVTHDPFYKRIVEETLEFIA"
BASE COUNT         1056 a          878 c          768 g         1001 t
ORIGIN      
        1 ctaacgaggc ggcacgcact tatgccggta tcagtgacgg catgccacat aactccgtgc
       61 tggaaagcat gttcaatatg tcggtcactg cgcatatttt gggtggctgc ccaatcggag
      121 tggatagtca aagcggcgtg atcgactcgc gccacgaagt ttttggatat cccggtctgt
      181 atgtgatgga tgggtcagcc atccctgcca atgtgggcgt caatcccagt ctcaccatca
      241 ccgcgctaac cgaacgcgcc atgagtttat ttcctcaaaa ataaggttga cagcattaag
      301 ggttctattt ccaaaaatgt gttaatttat cttccacctc atgaatgttt tttacaacct
      361 gatatccacg ccaatgagaa ggaaacaact ggcgatactg agtctgagta aagcgttcat
      421 cgattaaaac tatgattcct cgatctgtat ccgtgcgaat gacccgcccg gttgcctgta
      481 atacccgatt aaatcctgga tattgataag cataagcata ccctctacct agatcgcgat
      541 caaaatattc tttgatcaaa tctctttcca atcctagttg tggtaccccc actccaacaa
      601 cgataacccc gatcaatcga tctccaacca gatcaatgcc ctcaccaaaa acaccgccca
      661 tcacagcaaa cccgaccagc gtttcttcat tggtcgtgga aaactgtgct agaaaagaat
      721 cgcgggcatt ttcatccatt ccccgttcct gaattagaag ctgtctatct ggcaatctct
      781 gcttgagccg ttctacgacc tcatttaagt acacataaga tgggaaataa accaaataat
      841 tacccgtatg ggttttaatg atggtttcaa tcaactcggc aatatcgtca taagaatccg
      901 cacgttgtgt atatttagtg gatatcttgt tatgaattaa caaacaggca ttttcctgcg
      961 gaaaaggtga ttcaaaagta cggctgatat gatctgttgc acctgtcaac agtttcttga
     1021 aataattcat gggtaacaga gtcgcagaga aaaagattgt cgcacaacta cgttccaaag
     1081 gaaacgctaa cagtggagca gggtccagac agaatagttt ggcacgcaga tccgattgtc
     1141 cccgccgttc aaagtacgag acataatacg tgtcaaaata ttcagcagta cgaagaaaat
     1201 tactgcacag gaagtaaaac tccaacaact cctgcctaaa ttcagccacc tgattcataa
     1261 caagccaatc ttcagcctta tgactaaatt ctcggagtgt cttgagcaag gtttcaggca
     1321 aatcatgttc aatcaaggca tctttatctt ctgcctcaca agctttacgt ttctctaata
     1381 atattttgtt gacctctccc agctttttcg ccagagcagg cacatgtggc ttcaaggtac
     1441 gttgaaggct tagcacagtc tctttatcca actcagccga gtacatggct ctggcacgat
     1501 ctggtaggtt atgagcttcg tcaacaagaa agatataagg ctctgtcgtg aagtcaaaaa
     1561 agcgatggag atagacgcgt gggtcaaaaa catagttgta atcacaaatg atgcaatcca
     1621 cccataacgc tacatccaac gaaaactcga agggacaaat ctcatacttt cgggcgagtt
     1681 cttcaataac cgggcgcgta aaagtttggt gctgatccag ctcttgaagc gcaagtttca
     1741 ctttgtcaaa atagccacga gcaaatatac atacttccgg gtcgcagtta actggcgggc
     1801 aaaaacagat tttttccttg gcagtcaatg tcacgcttcg tagatacaaa ttcgaacggt
     1861 acatatcctc taaagctttt tccgcaacca atcgccctga cgtcttggct gttaaataaa
     1921 aaatcttggt tgccaatcct tgccccaagg ctttgaccga tgagaaaagg gtcgcaatgg
     1981 tctttcccac gcccgttggc gattggatat aagcacgatc attcgcacga atggttttat
     2041 agacagtgac tgccaaatct cgctgaccag ggcgatattc atcataggga aattcgagct
     2101 gctcaataga acgatcacgc cgagcttgcc aggcttggac cttcctgaac cagtccaagt
     2161 actgggtaat taggttcgag aaaaagcttt ccagttctgc ccatgtaaaa tggcgttcaa
     2221 atgttttttc ttcatggcta tccaattggt aataagttag atggacattg atttcactta
     2281 agttctgttg aagggcatac atatatgcat agcattgtgc ttgcccccaa tgcaatgggt
     2341 tgtgatcatc gtgtatcaga tccaagctca gtgttgttgt tttgatttct tcaatgatga
     2401 ccggttcttt gccagcatat aaaccatcaa tccgccctcg tatttccaat ggcggattgg
     2461 tattttcgat acgacaaatg atttctactt cggtgtcata tccctccggg cgggattttt
     2521 gtacttgttt atgtcccaga gttcctagct gggcgcgatc gcgtctttga aacccgcctg
     2581 ctgtcaggtc gccagattgc atcacgaatt ccactaggct gcgaacagaa atactatgag
     2641 tatggtctgc ttctaatgaa gcgttagggt tggtatccat tttcaaatgg gattctagca
     2701 cgataaacaa tatcttttta atatttataa agcgttgctc tagaccataa gctataatcc
     2761 catacaagtt aacgtataca tttactatag aggttacaca tgcctaacta tcttatttca
     2821 gcgagttctc catatttact tcaacacgca aacaatcctg tagattggta cccatgggga
     2881 gaagaagctc tcaataaagc caaagccgaa aataaaccga tttttctaag catagggtac
     2941 gccgcatgtc attggtgcca tgtaatggct catgaatctt tcgaagattc tgagaccgcc
     3001 gctttcatga acgaacattt catcaacatc aaagtagatc gcgaagaacg ccccgacctc
     3061 gataatatct acatgcaggc aaccatcgcc atgaccggct ccggcggctg gcctatgtct
     3121 gtctttctta cgcccgaact caaaccattt tatgctggca cctacttccc gcccgtgcga
     3181 cggtataaca tgccctcttt cagggaagtg ttagaaggtc tctccaatgc ttgggtaaat
     3241 gaccgcgaag aaattgagaa aactagcaac aaagtctttg aacatttaga tacaaaacta
     3301 aaatccaaca acgaagaaaa tcttacgaca gatcatctcg aagcaattgc caaagcaatg
     3361 caaggtgctt attactgggg aactggcggt tggggtgatg cgccaaaatt tccacagccg
     3421 atggctcttg aattcttgct tcatcacgcc gtcacaacaa agcaaaatga atacataaaa
     3481 cttatcgaac attgcctcaa agtaatggca cgcggcggga tgtatgatgt tgttggcggc
     3541 ggcttctcac gctatagtac agatgaccat tggcgcgtgc ctcattttga gaaaatgctt
     3601 tacgacaacg ctcaactcgt ccgcgtgtat ttgcatgcct ggcaagtgac acacgaccca
     3661 ttctacaaac gcattgtcga agaaactctt gaatttatcg cga
//
LOCUS       DAOQJE010000266         3697 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_15613_length_3697_cov_5.316859, whole genome shotgun sequence.
ACCESSION   DAOQJE010000266 DAOQJE010000000
VERSION     DAOQJE010000266.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3697)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3697)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3697
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15613_length_3697_cov_5.316859"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..790
                     /locus_tag="PLT08_08185"
     CDS             <1..790
                     /locus_tag="PLT08_08185"
                     /inference="COORDINATES: protein motif:HMM:NF024989.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="NAD(P)-dependent alcohol dehydrogenase"
                     /protein_id="HNE04481.1"
                     /translation="THPKDPRIGTDFAGVVEAVGSKVTHLKAGDEVYGGRTGAFAEYV
                     NVVNAVVRKPANVSFEEAAAIPTAAITALQGLRDHGKIKAGDKVLINGAAGGVGTFAV
                     QIAKAMGAEVTGVTSTKNVELIRSLGADTVIDYSKEDFTQTNKQYDILLDIAGSRTWK
                     EYRKVLKPKANFVIVGGQKGNKFIGPLANALRLRVSALGASQTVAFFIANFSRDDLNL
                     LRELVERGKVKPVVEKVYPLAQAADAMKHLGTGHARGKIVLKVK"
     gene            871..2421
                     /locus_tag="PLT08_08190"
     CDS             871..2421
                     /locus_tag="PLT08_08190"
                     /inference="COORDINATES: protein motif:HMM:NF012488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HNE04482.1"
                     /translation="MEITYFEFVKHLIRFNRNVYLDHNATTPVSEHVRNKMDQALKYH
                     YGNPSSFYGSGRKSAGLVEEARGHVANAIHAEPYEVIFTACATESNNAVLKSVSAHFY
                     PQKKKIISTPIEHPSVLNTLAYLETQGVVVEFCPVDNKGRIQLTHLENMVDNDTFLIC
                     CILANNETGVLQDIQAVTKIAKSHGALVLTDCVQALGKIPIDVHTWDVDYASFSAHKL
                     YGPKGVGALYVKQGSPFTPLLHGGHQEGGLRAGTESTHNIVGFGAACQQVDKLLAHVE
                     KVRALKYDLIQRLKDIKPDCVINSPEVDSECLPNTLSIRFPGVENSGLMGMLDYRGIA
                     VSAGSACSTGEDTPSHVLKAIGLSDQAARETIRISLGHDTSARDIQYTANVIRDYLEG
                     RISFVNMLAPAQLNETILFSDDTFILDVRPSDDRAKFKGIPNAHEVNPLFVENYIKQL
                     PRDKQIIVYCPGGGLSVMISYFLKAKGFKRITNLRGGLDAWRKRRADLYGKYAGQNVT
                     VLEPDAKR"
     gene            2552..3028
                     /locus_tag="PLT08_08195"
     CDS             2552..3028
                     /locus_tag="PLT08_08195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE04483.1"
                     /translation="MNKGPFIVLRVLGILLILGLIAGAGAFGYKAGLAQGIQQAPEVA
                     AAFEKAAENGQVAMPGAPMMYGYGYPHMYGFGHPHFFNPFGAICFSFLFLFLFFGAMK
                     MLFFRRMRHGWGHHGHHGPWRKDWEGNVPPFFNEWHKRAHGESTEGETPADSEKKE"
     gene            3129..>3697
                     /locus_tag="PLT08_08200"
     CDS             3129..>3697
                     /locus_tag="PLT08_08200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HNE04484.1"
                     /translation="MPELILVVDDEPKIVRIARDYLEKNGFRVITAADGHAALATARR
                     EKPDLIVLDLMLPLMDGREVCKTLRRESDVPIIMLTALAEEVDQVTGLEIGADDYITK
                     PFSPRALVARVRALLRRAKGEVKPPSIIRVGGLEIDPDRYSVTFNDEPVKLTPNEFKL
                     LQLLSARPGQTLTREQLLEDMHGIASSFDR"
BASE COUNT          969 a          923 c          923 g          882 t
ORIGIN      
        1 cacccacccg aaagatccgc gcattggcac agattttgcc ggcgtggtgg aagcggttgg
       61 cagcaaggtc actcatctca aagcgggtga tgaagtgtat ggcgggcgga cgggggcttt
      121 tgctgaatat gtcaacgtag taaatgcagt ggtgcgcaaa ccagccaacg tctcatttga
      181 agaggcggca gccatcccta ctgcggcgat taccgctcta cagggattaa gagatcacgg
      241 caagatcaaa gctggagata aggttctcat caacggcgcg gcgggaggcg tgggcacgtt
      301 cgcagtgcaa attgcaaagg caatgggagc cgaagtgacc ggtgtgacca gcacgaaaaa
      361 cgttgagtta atccgctcac tcggcgcgga tactgttatt gactacagca aagaagattt
      421 cacccaaact aataagcagt atgacattct cttggacatt gccggaagcc ggacgtggaa
      481 agaatataga aaagtcctca aacccaaagc aaactttgtc attgttggtg ggcagaaggg
      541 caataagttt attggtccgc tggcaaatgc gcttcggttg agagtttcgg cattgggtgc
      601 cagccaaact gttgctttct tcattgccaa tttcagccgt gacgacctca atcttttacg
      661 agagctagtt gaaaggggaa aagtaaaacc cgtcgtggag aaagtctatc cgctggcgca
      721 ggcagccgac gccatgaagc atttggggac ggggcatgca cgcggcaaga tcgtgctcaa
      781 ggtgaaataa gagggcatcc attaccaact attacttcgg aacaacgtat actgagagta
      841 accgtatcat agctttatcc cggagtctca atggaaatca catactttga attcgtaaaa
      901 cacctcatcc gcttcaaccg caacgtttat cttgaccata atgcaacgac accggtcagc
      961 gaacacgtac gcaataaaat ggatcaggca ctgaaatatc attatggtaa cccttcgtca
     1021 ttttatggca gtggaagaaa atcagctggg ttagtagaag aagcccgagg gcatgtggca
     1081 aatgccattc atgccgagcc gtatgaggtg atttttacag cgtgcgctac tgaatcaaat
     1141 aatgcggtac tgaaatctgt ttcagcgcac ttttaccctc aaaagaaaaa gatcatttca
     1201 acaccgattg aacacccttc tgtgttgaat acattggcat atttagaaac acagggtgtg
     1261 gttgtcgaat tttgcccagt ggataacaaa gggcgcatcc aactcactca cctcgaaaat
     1321 atggttgata acgacacctt tttaatttgc tgtattctag ccaacaatga aaccggcgtg
     1381 cttcaagata ttcaggcggt tacaaaaatt gctaaatcac acggagcgct ggtgttgaca
     1441 gactgcgtgc aagccttggg aaagatacca attgacgtcc acacttggga tgtagattac
     1501 gcctcttttt cggctcacaa gctgtacggt ccaaaaggag tcggagcgtt atacgtcaag
     1561 cagggaagcc catttacacc actgctgcat ggtgggcatc aagaaggcgg gctacgggca
     1621 gggacagaaa gcacccacaa tatcgtcggc tttggcgccg cctgtcaaca agtggacaaa
     1681 cttctagcac atgttgaaaa agtccgtgca ttgaaatatg atttgattca acgtctcaaa
     1741 gatatcaaac cagattgcgt tatcaattcg cccgaggtgg attcggaatg tctaccgaac
     1801 acactcagca tccgtttccc cggtgtggaa aattccgggc tcatgggcat gttggattat
     1861 cgtggaatcg ccgtctctgc tggctcggcg tgcagtactg gtgaagatac gccatcgcat
     1921 gttttgaaag caatcggttt gtctgaccaa gccgctcgcg aaacgattcg catcagtctc
     1981 gggcacgata cttcggcgcg agatattcaa tatacagcaa acgtaattcg agattatctc
     2041 gaaggtcgga tttcgttcgt gaatatgctc gctcctgcgc agctgaacga gacaatcctt
     2101 tttagcgatg acacgtttat tctcgatgtg cgtccctctg atgaccgggc aaagttcaaa
     2161 gggataccca atgcacatga ggtcaacccg ctgttcgttg aaaactatat aaagcaactc
     2221 ccccgcgata agcagattat cgtctattgc ccgggcggag gtctttcggt catgatctct
     2281 tatttcttga aagcaaaagg gtttaaacgc atcaccaatc tgcgcggcgg gcttgatgcg
     2341 tggcgcaaac gacgggctga cttgtatgga aaatatgcag ggcaaaacgt gacagttctt
     2401 gagccagatg ctaaacgtta agcaagaggc gctaataatt ttcctacatt ccctccacaa
     2461 tccctcacct gtccatccat aatccctcca caatgggttg atacatttag acgcgtaagg
     2521 acaaatcaaa caattaccat tggaggtaaa aatgaataaa ggtccgttta ttgttttacg
     2581 cgttctcggt attctgctca tcctcgggct gatcgcaggc gcaggggcgt ttgggtataa
     2641 ggctggtttg gcacaaggga ttcaacaagc tcctgaagtt gccgccgcct ttgagaaagc
     2701 cgccgagaat ggtcaggttg ctatgcccgg cgcacccatg atgtacggat atggctaccc
     2761 gcatatgtac ggttttggtc acccgcactt cttcaatccg ttcggcgcga tctgcttctc
     2821 gttcttgttc ctcttcctgt tcttcggcgc aatgaagatg ctcttcttcc gccggatgcg
     2881 acatggctgg ggtcaccacg gacatcacgg tccctggcgc aaagattggg aaggcaacgt
     2941 ccccccattc ttcaatgaat ggcacaagcg cgctcacggc gaaagcacag aaggcgaaac
     3001 acctgccgac tctgagaaga aagagtaacg tgtgaaataa agaagaaaga ggaagaaacg
     3061 cctctttctt cttccccttt cttaagccct cactatccat ccttcctcat tccctttata
     3121 ctttccccat gcctgaactg atcctcgttg ttgatgatga acccaagatc gtgcgtatcg
     3181 cgcgcgacta tctggaaaaa aatggctttc gcgtgattac ggctgccgac ggtcacgccg
     3241 cgctggcaac ggctcgccgc gaaaagcctg atctgatcgt tttggacttg atgctccctc
     3301 tcatggacgg gcgtgaagtg tgcaaaactc tgcgccgtga aagcgacgtc cccatcatca
     3361 tgctcaccgc tctcgccgaa gaagtggatc aagtcaccgg gctggagatt ggtgccgatg
     3421 attacatcac caaaccgttt tcgccgcgcg ccttggtcgc gagagtccgt gccttgttgc
     3481 gacgtgcaaa aggtgaagtg aaaccaccta gcatcatccg tgttggcgga ttggaaattg
     3541 accccgatcg ctactcggtc acattcaacg acgagcccgt caaacttacg ccaaatgagt
     3601 tcaaactttt acagttgctt tccgcccgcc caggtcagac cctgacccgt gaacaattac
     3661 tcgaagatat gcacggcatc gcatccagct ttgaccg
//
LOCUS       DAOQJE010000267         3692 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_15650_length_3692_cov_8.144900, whole genome shotgun sequence.
ACCESSION   DAOQJE010000267 DAOQJE010000000
VERSION     DAOQJE010000267.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3692)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3692)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3692
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15650_length_3692_cov_8.144900"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1480)
                     /locus_tag="PLT08_08205"
     CDS             complement(<1..1480)
                     /locus_tag="PLT08_08205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HNE04485.1"
                     /translation="MSWSYGEVLKPETINYRRLRPEKDGLFCEAIFGPTRDWQCYCGK
                     YKNPRYKGITCEKCGVEVTRSSVRRERMGHIGLASPVAHIWYTRRIPSQMGMLLDISR
                     RNLDRVLYFAQYIVTYVDEEARKKALQRLEDEITVSEREQASEINNRIIEIKQKRDAS
                     ISELKQKQATLEQGYDEGIAEQLDPVIKEGQKLERLLQDQMGEAAKKAIVFEQTGEKI
                     IDAGDKVASKHITLIQKTVQKKLESLENELKDKRQAEIDDLKTKAATIKAQADTEMEA
                     LRNQLESQTSVSSNNSSRLRDELLELRPFTFLSEIRYRELKQRWGQVFRADMGAEAFY
                     DVLERLDLDKLSEELWHEVKTTKSKQKRKKATTRLKVVEAFKRSGNRPEWMILTVLPV
                     IPPDLRPMVQLDGGRFATSDLNDLYRRVINRNNRLKRLLELGAPDVIIRNEKRMLQEA
                     VDSLIDNSQRGKALSRRGRRELKSLSDMLKGKKGRFRRNLLGK"
     gene            complement(1785..>2348)
                     /locus_tag="PLT08_08210"
     CDS             complement(1785..>2348)
                     /locus_tag="PLT08_08210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE04486.1"
                     /translation="GADFSQKTIPGWETVNDDKLHFHKGPPAELRGKFTPQVNSYYML
                     KSSGFLDNFDVSSTLRFFDGDKEWIRAGYFFRHTPEGGYGLMVSAQGSYTFGYVAKDE
                     KNELLFYKIMPWSWHTALRSGMNVFNRLRVICNGSSFRIYLNGVLATSFQDERFKMGR
                     IYLAAEPSEKSSMELGFSDLQVREVPK"
     assembly_gap    2349..2448
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2449..>3692)
                     /locus_tag="PLT08_08215"
     CDS             complement(<2449..>3692)
                     /locus_tag="PLT08_08215"
                     /inference="COORDINATES: protein motif:HMM:NF013100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HNE04487.1"
                     /translation="AVKALEYVGIYDDPNTPEKDGMIAMAERLGITSLTRTDYGLSLT
                     LGGGDVSLFDMTSAYAVFANGGKKLPPVAILKITTFDGEVVYEYQQPEGEQILRPEHA
                     YLISSILSDNEARSYTFGRNSALNLSFPVAAKTGTTNDIRDNWTMGYNPDLVTGVWVG
                     NADYTPMVNSSGLSGAAPIWSQFMEFAVPYLTNGAPTPFSRPSGIVDKVVCRLGGTEP
                     TNECGGEYTEVFASDQLPPTKDQGLVRRVNLDVWTGYEASDACDGPTDEKLVMNVKDP
                     WALKWFDTGDGKAWLTDHGLDENLDIVPDRKCEEGDPQPEIEINLSDGQVISAPVVEV
                     KGTASASGNFKQWRLEFGIGEDPGSWTTLSESNNPVEGGTFTTWDVSNLPNGIITLRL
                     TLVGERTDISKRVRLNLSLPIPT"
BASE COUNT          842 a          902 c          930 g          918 t
ORIGIN      
        1 gcttaccgag caggttgcgg cggaagcgac ccttcttgcc tttgagcata tcgctcaagg
       61 acttgagttc gcggcgtccg cggcgggaga gagctttacc gcgctggctg ttgtcgatca
      121 aagagtcaac agcttcttga agcatacgct tctcgttgcg gataatgaca tctggcgcgc
      181 cgagttcgag caatctcttc aagcggttgt tgcggttgat cacgcggcga tagagatcgt
      241 tgagatcgga agtcgcaaag cgaccaccat ccaactgcac catcgggcgc aggtcgggag
      301 ggatcacggg caatacggtg agaatcatcc actcagggcg gttgccagag cgtttgaacg
      361 cttccacaac cttgaggcgg gtggtagctt tcttgcgctt ctgcttgctc ttggtggtct
      421 ttacttcatg ccagagttct tcagagagct tatcgaggtc gaggcgttcg agaacgtcgt
      481 agaaggcttc agcacccatg tcggcgcgga agacctgacc ccaacgctgc tttaattcac
      541 gatagcgaat ttcgctcaag aacgtgaacg gacgcagttc aagcaattcg tcacgcaagc
      601 gggaagaatt gttcgaagag accgaagtct gcgattccaa ctggttgcgc aaagcttcca
      661 tctcagtatc agcctgcgct ttgatggtag cagccttggt cttgagatcg tcgatctcag
      721 cctgacgctt atccttcaac tcgttctcaa gcgattcaag tttcttctga actgtcttct
      781 ggatcaaggt aatgtgcttg cttgcaacct tgtccccagc gtcgatgatc ttttcgccgg
      841 tctgttcgaa gacaatagcc tttttcgcag cttcacccat ctggtcctga aggaggcgtt
      901 cgagtttctg accttctttg atgaccgggt caagctgttc agcaatacct tcatcgtaac
      961 cctgttcaag ggtcgcttgc ttttgcttca gttcactaat gctggcatca cgtttttgtt
     1021 tgatctcgat gatgcgatta ttgatttctg aagcctgttc gcgttcagaa acggtgatct
     1081 catcttcaag gcgctggagg gctttcttgc gggcttcttc gtccacgtag gtaacgatat
     1141 attgagcaaa gtaaagcaca cggtccagat tgcggcggga aatatccagc aacataccca
     1201 tctgagatgg aatgcggcgg gtataccaaa tgtgcgcaac cggagaagcc aacccaatat
     1261 gacccatgcg ctcacggcgc acagaagaac gggttacttc aacaccacac ttttcacaag
     1321 taataccttt gtaacggggg ttcttgtact taccgcaata acactgccaa tcgcgggtag
     1381 gtccaaagat cgcttcgcag aacaagccgt ctttctcagg gcgcaagcgg cgatagttaa
     1441 tggtttcggg tttcaacacc tcgccataag accaggacat aattgtctgg ggtgaggcaa
     1501 ggctgatacg gagtgctgtt aatcccttgg tttccactgg atcctctcca aaaaataaat
     1561 ttcgtttgag cgatacggta gcgcatagat aacgcgtacc tgagacaaac aaatgcaagc
     1621 gctctgaggt ttttcctcaa gcgcttgctg gtgttttctc gttttttaca tccaacttag
     1681 caattatatg gattcaggca ctgagagtca aggttaatca gtgaagagag gcatacttta
     1741 aggcggattc agcgcctctt tcccgtgcca aagcctgatt ctgcctattt tggcacttcc
     1801 ctcacctgca aatcggagaa tcccaactcc atagaagact tctcgcttgg ctcagcggca
     1861 aggtagatcc tacccatttt gaaacgctca tcctgaaacg acgtggcaag cacaccgttc
     1921 aagtagattc gaaaactgct gccattgcat atcactcgca agcggttgaa gacattcatg
     1981 ccagaacgta acgccgtatg ccacgaccat ggcatgatct tataaaaaag caattcattt
     2041 ttttcatctt tggcaacata accaaacgta tacgaaccct gtgccgacac catcaaccca
     2101 tacccacctt caggagtatg gcggaagaag taccccgctc gaatccactc tttatcgcca
     2161 tcaaaaaagc gcagggtaga acttacatcg aagttatcta aaaagccaga actctttagc
     2221 atgtaatacg agttgacctg tggcgtgaat tttccgcgca actcggcggg cggaccttta
     2281 tggaaatgta acttgtcgtc gttgaccgtt tcccaaccag gtatggtttt ctgggaaaag
     2341 tctgcgccnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2401 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnngt tggaatgggc
     2461 aggctcaaat taagtctgac gcgtttactg atatccgtgc gttctccaac cagagtcaag
     2521 cgcaaggtta tgattccgtt tggcagattc gaaacatccc aagtggtgaa ggtgccgccc
     2581 tctacagggt tgttgctttc tgagagggtc gtccatgaac cggggtcttc gccgatgccg
     2641 aattctaaac gccattgttt gaagttaccg ctggcagagg cagtgccttt gacttcaacc
     2701 acgggggcag aaatgacttg accatcggag aggtttattt cgatctctgg ttgcgggtca
     2761 ccttcttcac acttgcggtc tggcacgatg tctaggtttt catccaagcc atgatctgtt
     2821 agccaggctt ttccatcgcc tgtatcaaac catttgagcg cccacgggtc tttgacgttc
     2881 atgacgagct tttcatcggt gggaccatca catgcgtccg aggcttcata gccagtccaa
     2941 acgtccaggt ttacgcggcg tacaagtccc tgatcttttg tgggaggcaa ttggtctgat
     3001 gcgaagactt cggtatattc gccgccgcat tcattggtgg gttcggttcc gccgagacga
     3061 cagacaactt tatccacgat gccagatgga cgcgagaaag gagtcggcgc gccgttggtg
     3121 agatagggca cggcaaattc catgaactgt gaccagatcg gagcggctcc gctcaagccg
     3181 gatgaattga ccatgggggt gtaatcagca tttccaaccc acacgcctgt gaccaagtct
     3241 gggttatagc ccatcgtcca gttatcgcgg atgtcgttgg tggtaccggt tttggctgcc
     3301 accgggaagg aaagattcaa tgcagagttg cgaccaaagg tgtagctacg cgcttcgtta
     3361 tctgaaagaa tggaggagat gagataggca tgttcagggc gaaggatctg ttcgccttcg
     3421 ggctgttgat attcataaac cacttccccg tcaaaggtgg tgatcttaag aatggcgact
     3481 ggcgggagtt tcttgccgcc attggcaaag actgcatagg cactggtcat atcgaagagg
     3541 ctgacatcgc cgccgcccaa ggtgagggac agaccataat cggtacgcgt gagcgatgtg
     3601 atgccaaggc gttctgccat ggcgatcatg ccatcttttt cgggggtgtt cgggtcatcg
     3661 taaatgccga catattccaa tgctttgacg gc
//
LOCUS       DAOQJE010000268         3687 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_15691_length_3687_cov_2.973293, whole genome shotgun sequence.
ACCESSION   DAOQJE010000268 DAOQJE010000000
VERSION     DAOQJE010000268.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3687)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3687)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3687
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15691_length_3687_cov_2.973293"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..447
                     /locus_tag="PLT08_08220"
     CDS             <1..447
                     /locus_tag="PLT08_08220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE04488.1"
                     /translation="GSVSGLDELPGDMPLFVGNGRAPYKLFWELRYQEYEAIEGCLAG
                     PNGNGNYNCGGGTGHRGITGYEWKNHSSGGEIPPSAVANLPAARMADINNDGTPDAYW
                     DTNLTLRRMDDAGSISNPQYQRSWNWGGIIYWAVREGQGQIGWPGQ"
     gene            complement(566..1267)
                     /locus_tag="PLT08_08225"
     CDS             complement(566..1267)
                     /locus_tag="PLT08_08225"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013594.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metalloprotease"
                     /protein_id="HNE04489.1"
                     /translation="MQVIQPILILSITSLLGWILLYCNYYYQINKLNNPRDTNLENYP
                     NSLRTMIDNFNPDIKLIIVKGSITETRAVTFGFFPSYIVLSQRLFEKGKSEFVEFNTE
                     DLHAIVAHELGHIIPNSKLTLIMWLCFSAFLAIFVSVQYSLLLSLCMGFSFFILFAWL
                     SLSSRHEEYQADAFAITIAAISMEQLVNCLKKLDLVFRREIPLRQRQSIFRRLFRPTL
                     EQRIFQLYQNNKISH"
     gene            complement(1285..2385)
                     /locus_tag="PLT08_08230"
     CDS             complement(1285..2385)
                     /locus_tag="PLT08_08230"
                     /inference="COORDINATES: protein motif:HMM:NF013862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HNE04490.1"
                     /translation="MIGLALSGGGSRAAAFHLGCLRALDDLGLLNRIQVISTISGGSI
                     IGAYFAYSPNKSFSDFDSDICMLLRNGLQKEIVLEVLKPGNLIRSSMNGLAEKIDRFL
                     SNIIKKDPSLLRYPTRTDMFIDVLNRELFAGLKVSAKRRNDMDIVIGACELRTGTAFR
                     FSNSRAGDWKHGELIDWDIDLACAVAASAAYPLLLPALDRRWRFHKNGQDTFHRVILT
                     DGGVYDNLGVQVLEPNRDPDISLHTFPCEYLVVCNAGRGQDEVLSMPVDFVPRVARSL
                     EIAHRRVQESTMRRLHHLRETGKIKGFVMPYLGQQDSKLLNLPADFITRSDVMNYPTD
                     FAPMSDDWIRKLAGRGEQLTKNLIYRYLTELL"
     gene            complement(2382..>3687)
                     /locus_tag="PLT08_08235"
     CDS             complement(2382..>3687)
                     /locus_tag="PLT08_08235"
                     /inference="COORDINATES: protein motif:HMM:NF013094.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ThiF family adenylyltransferase"
                     /protein_id="HNE04491.1"
                     /translation="VRRDNGTPMSWFNGKKVLILGCGALGSWAAEIIARANPCQIELV
                     DDSLVKPGILARQNFESRHIGLNKSIALADRLSAITFGCSVIPHPDEALKFLLSDIAR
                     FQEYDVVIDCTASNIFQSKLERDWNKFGSRTPGLISLIIDSQAKHTLCVVVPQNAPDG
                     IWNAYLGLKLRLTSENGLPNVASAFYSTRATDGMFQPEPGCSDPTFVGSTADIFALVS
                     NALNWAISNLQGINPGGALALSTLVHNGKRDSDVVDLPHFREVQVGSYKVRITPNIFT
                     QAKAWIKQNHRSRSQQHETGGLLWGFWDDAIGVIWIFDLSGPPSDSRHDPGHFICGVK
                     GTQEEHGRRSDKSFGLCGFIGHWHTHPNLPSYQSPVDMSSMINLVSTVGQNQKRSLMM
                     ILGSVHEMDTVGIYVYESQPLKRNHEMITVGSVQFDLDGDRT"
BASE COUNT         1080 a          841 c          764 g         1002 t
ORIGIN      
        1 ggcagtgtca gtggactgga tgaactcccc ggagacatgc ctttgttcgt cggcaatgga
       61 cgcgccccct acaaattgtt ctgggaattg cggtatcagg agtatgaagc catcgaaggc
      121 tgccttgccg gtccgaatgg caacggaaat tacaactgcg gcggcggaac cggacatcgc
      181 ggcatcacag gctatgaatg gaagaatcat tccagcggag gcgagattcc tccgtctgca
      241 gtggcgaact tgcctgccgc aagaatggcg gatatcaaca atgatggtac gccggacgcc
      301 tactgggata ccaacctgac cctgcgacgc atggatgatg ccggcagtat cagcaatcct
      361 caatatcagc gttcctggaa ttggggcggg atcatctatt gggcggtgcg tgaaggacag
      421 ggacagatcg gctggccagg gcaataatct aaaatttggt tatcagccta tgatatacaa
      481 aaagcatgcc ataggctgat aaatcgatcc aacaagttta tgcagccaat actttatttt
      541 ttggagggta gaaataatag ttgtattaat ggctgatttt attattttga tatagctgaa
      601 aaatgcgttg ttctagtgtc ggacggaaca acctgcgaaa tattgattgg cgctgtctta
      661 aaggaatttc tctcctaaaa accaaatcaa gtttttttaa acaattaaca agctgttcca
      721 ttgaaattgc cgcgatagtt attgcaaacg catccgcctg atattcttca tgccgcgaac
      781 taagcgacag ccaggcaaat aggataaaaa acgaaaatcc catgcagagg gacaataaca
      841 aactgtattg cacagaaaca aatattgcaa ggaaagcgct aaaacacagc cacattatca
      901 acgtaagttt gctgttgggg ataatatgac ctaattcatg cgccactatt gcatgaagat
      961 cttctgtatt aaattctaca aattcagatt tccctttttc aaatagtcgt tgcgataaaa
     1021 cgatatagct cggaaaaaag ccaaaagtca ctgctctcgt ctccgttatt gaccctttga
     1081 ctattatcag ctttatgtca ggattgaaat tatcaatcat cgttcgtaaa gaatttggat
     1141 aattttccag attggtatct cttggattat tgagtttgtt gatttgataa taataattac
     1201 agtaaagcaa tatccatccc aacaaggaag ttatacttaa gattaaaata ggctgtatta
     1261 cttgcattga atcaatccaa agtgtcaaag caattcagtt agataacgat aaatcaaatt
     1321 ttttgtcaac tgttcgccgc gcccagcaag tttcctgatc caatcatcac tcataggcgc
     1381 aaaatcagtc ggataattca tgacatcgga tctagtaata aagtccgcag gtaagtttag
     1441 taatttacta tcttgttgtc ccagatatgg catgacaaaa ccttttattt tgcccgtttc
     1501 tcttaagtgg tgaagccggc gcattgttga ttcttggact cttctatggg ctatttccaa
     1561 tgaccgtgca actctaggga caaaatcaac aggcatagat aaaacttcat cctgtcccct
     1621 cccagcattg cataccacca aatattcaca tggaaatgtg tgaaggctaa tatcggggtc
     1681 tctgtttggc tccagaacct ggacacccag gttatcataa actccgccat cagtcagaat
     1741 aactctgtga aaggtgtctt gtccattttt atgaaatcgc catctgcgat caagcgcagg
     1801 caaaagaaga ggataagcgg ctgatgcagc aactgcacag gccaaatcta tatcccaatc
     1861 tataagctca ccatgtttcc agtcccccgc tctactattg ctaaatctaa atgctgttcc
     1921 tgtacgcaac tcacaagcgc caattactat atccatatca tttcgacgtt tcgcgctcac
     1981 cttaagccca gcaaataact cacgatttaa aacatcaata aacatatcag tacgagttgg
     2041 gtagcgaaga agtgaaggat cttttttgat gatgtttgac aaaaaacggt caattttttc
     2101 agccaatcca ttcatgctac tacggataag attcccaggt tttaacactt caaggacaat
     2161 ttccttttgc aatccattcc ttaagagcat acatatatcc gagtcgaaat ctgagaaact
     2221 cttattcgga gagtacgcaa agtaggctcc aataatcgac ccgccggaaa ttgtcgaaat
     2281 tacctgaata cggttaagca atcccagatc atcaagtgct cgaaggcagc caaggtggaa
     2341 tgcagctgca cgcgagccgc caccggaaag cgctaaccct atcatgttct gtctccgtca
     2401 agatcaaact gcaccgagcc tactgtaatc atctcatggt ttcttttgag tggctggctc
     2461 tcgtaaacat aaattcccac agtgtccatt tcatgaacgc tacccagtat catcataaga
     2521 gacctctttt gattttgtcc taccgtcgaa accagattga tcatactact catatctaca
     2581 ggactttgat atgaaggtaa atttggatgt gtatgccaat gtccaataaa accacataat
     2641 ccaaaacttt tgtcagagcg acgaccatgt tcctcctgcg tccctttcac accacatatg
     2701 aaatgccctg ggtcgtgtcg gctgtccgat ggtggaccag ataagtcaaa aatccaaatt
     2761 actccaattg catcatccca aaatccccac aacaagcctc ctgtctcatg ttgttgtgaa
     2821 cgggagcggt ggttttgctt tatccaggct ttggcttgtg taaagatatt gggcgtaatc
     2881 ctgaccttat agctgccgac ctgtacctcc cggaaatgcg gcaaatccac tacatctgaa
     2941 tcgcgtttgc cattatgaac tagagtagac aacgctaacg caccacctgg atttataccc
     3001 tgtaaattac tgattgccca atttagggca ttggaaacta atgcaaaaat gtccgcagtt
     3061 gatcctacaa aggttgggtc agagcacccg ggttctggtt gaaacatgcc atcagttgca
     3121 cgggttgagt aaaaagctga tgcaacattg ggtaatccgt tttcgctggt caggcgcagt
     3181 ttcagcccca agtaggcatt ccagattcca tccggtgcgt tttgaggcac aacaacacaa
     3241 agagtgtgtt ttgcttgcga gtcaatgatc agtgaaatca gacccggggt ccgtgaaccg
     3301 aacttgttcc aatctcgttc aagtttactt tggaatatat ttgatgcagt gcaatcgatg
     3361 actacatcat attcctgaaa acgtgcaata tcactcaata agaatttgag agcttcatct
     3421 gggtgtggga taactgagca cccgaaagta atagcactta ggcgatcagc aagcgcaatg
     3481 gatttgttga gacctatgtg ccgagattca aaattctgcc tagcaaggat gcccggtttt
     3541 accaacgaat catccactaa ctcaatttga catggatttg ccctcgctat gatctcggcg
     3601 gcccatgatc ctaatgctcc gcatccgaga attaatactt ttttcccgtt aaaccaagac
     3661 ataggagtgc cattgtctcg gcgaaca
//
LOCUS       DAOQJE010000269         3684 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_15711_length_3684_cov_4.235878, whole genome shotgun sequence.
ACCESSION   DAOQJE010000269 DAOQJE010000000
VERSION     DAOQJE010000269.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3684)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3684)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3684
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15711_length_3684_cov_4.235878"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(180..1799)
                     /locus_tag="PLT08_08240"
     CDS             complement(180..1799)
                     /locus_tag="PLT08_08240"
                     /inference="COORDINATES: protein motif:HMM:NF015722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HNE04492.1"
                     /translation="MSETKRPLKVFLCHASADKPAVLDLYKRLVADGVDAWLDAESLI
                     AGQKWEVEIPKAIRDSDVVIVCLSEKSINKEGYVQREIKFALDIADEKPEGMIFIIPA
                     KLEECKVPDRLSTYHWVELIEEEGYRRLMRALRMRADKIDVTLQVPNSWLQSHLEEPA
                     QKIALDSKLLQTQVREFVRLINSRFLKKISPRIIIAILASLTFVIVAALKPEFFSQPP
                     IFSTPTATTIIPPSLTFNHTLPAKTFTPSKTPTKTATLEVMQTFTPSITITQKSASAT
                     LSFPVEITDAKGVQMVYVPSGSFTMGYGGGVNQAPTRKVFLSSFYIDKYEVSEKMYAQ
                     CVQDEACEIPNAGVWRTIDQYIDAKVNDNFPVVHVNWEMAGKYCEWREARLPTEAEWE
                     KAARGSGNSLFPWGSVYNGNVANLCDKNCESVSSNSDFNDGYGWLAPVDAFSRGASSY
                     GVYNMVGNVNEWVGDLYSTYYIRDQLTNPKGPTATTSNSHVIRGGAYNSDEWPLPVSI
                     SRWLSSNGGKDIGFRCAYDLKSDDSSNDDLP"
     gene            1901..2770
                     /gene="tcmP"
                     /locus_tag="PLT08_08245"
     CDS             1901..2770
                     /gene="tcmP"
                     /locus_tag="PLT08_08245"
                     /inference="COORDINATES: protein motif:HMM:TIGR04474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="three-Cys-motif partner protein TcmP"
                     /protein_id="HNE04493.1"
                     /translation="MDKYLLPEDDGLPARTFGKWTTAKTDYLQRYVDLFETSMRGKPW
                     CARCYIDLYSGTGKYQVEGQTGFHLGSPLIGITTKYPFTHYFFADISQENIDTLKQRC
                     DPAIQLRKKFYLGDANLLAQDIVNEIKSIERNRSKEQWASLNLAFLDPNGLELEWSTI
                     ETLASVNKMDLIIYYSQSGLTRNFENCINVEGDTDIDRFFGDREWRDIYKTSHGKSAG
                     LHRKLIDHYKHKLSTLGYMDVKDVEDGAEPLMKNSKDAPLYRLIFASKHARGHDFWNK
                     IIKKDAFGQEKLF"
     gene            2838..3560
                     /locus_tag="PLT08_08250"
     CDS             2838..3560
                     /locus_tag="PLT08_08250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage Gp37/Gp68 family protein"
                     /protein_id="HNE04494.1"
                     /translation="MAEHSGIEWTESTWNPLTGCTKISPGCKHCYAERMAKRLQAMGQ
                     PNYVNGFKLTLHENAMEKPLEWKKPQIIFVNSMSDLFHKDVPVEFVLEIFDVMRRAHW
                     HTFQVLTKRAERLEELSPKIDWPENVWMGVSVENQDYTYRIDHLRRTGAKTKFLSLEP
                     LLGPLHKMNLKGINWAIVGGESGPGARPLDPAWVTDIRDQCLRAKVPFFFKQWGGVQK
                     KKAGRVLEGRTWDEMPVNLVRA"
BASE COUNT         1112 a          821 c          751 g         1000 t
ORIGIN      
        1 acttgtgtca ttaggtttga ttcttcgttt tgaaagagta taacatggtg aaaaagccta
       61 actgtggcat gatacatcag tacttttttt atgaagttta cactttgagc agcttaggat
      121 caggtatagc acggcagaaa cacataactt aactaaaatc tattgtagaa tttttcaact
      181 caaggcaaat catcattact agaatcgtct gatttcaaat cataagcaca cctaaaccca
      241 atatccttac cgccatttga agataaccaa cggctaatag aaaccggcaa aggccattca
      301 tcagaattgt aggctccccc acgaattaca tgcgaatttg aggtagtcgc cgtgggaccc
      361 ttgggattcg ttaattgatc ccttatgtaa taagtagaat acaaatctcc aacccattca
      421 ttaacattcc ctaccatatt gtatacacca tacgaacttg caccgcgaga aaaagcatca
      481 accggagcca accatccata accatcatta aagtcggaat ttgaactaac actttcgcag
      541 tttttatcgc acaaattagc cacatttcca ttgtatacac ttccccaagg gaaaaggcta
      601 ttcccgctcc ctctagcagc cttttcccat tcagcctcag tcggcaaacg agcctctctc
      661 cattcacaat atttaccagc catttcccag ttgacatgta caacaggaaa gttgtcattt
      721 acttttgcgt ctatgtactg atctattgtt ctccacactc cggcgtttgg gatttcacac
      781 gcctcatcct gaacacattg ggcatacatt ttttctgaaa cctcatattt atcaatataa
      841 aatgaactca aaaaaacttt acgggttggc gcctgattaa ctcccccgcc atatcccata
      901 gtgaaagagc cgcttggaac atacaccatt tggacgcctt tcgcatcggt aatctcaact
      961 ggaaaagaga gagtggcact tgctgatttt tgcgtaattg taatgctcgg tgtaaacgtt
     1021 tgcatcactt ccaaagttgc ggtttttgtt ggtgttttac taggagtaaa agtttttgct
     1081 ggcagggtat gattaaatgt cagtgatggc ggaattatcg ttgtggctgt tggtgttgag
     1141 aatataggag gctgagaaaa aaattctggc tttaaagcgg caacgataac gaaagttaaa
     1201 cttgcaagaa tagcaataat aatacgggga cttatttttt tcagaaatct tgaatttatt
     1261 aaccgtacga attccctcac ttgcgtctgt aaaagcttac tatcgagtgc aatcttttgt
     1321 gctggctctt caagatgcga ttgaagccaa ctattaggaa cttgtaaagt tacatcaatc
     1381 ttatctgccc gcatccgcaa agcgcgcatt agtcttctgt atccctcttc ttcaatcaac
     1441 tctacccagt gatacgtgct tagcctgtca ggaactttac actcttctaa tttagcagga
     1501 atgataaaaa tcattccttc gggtttttca tctgcaatat ccagagcaaa cttgatttcc
     1561 ctctgaacat acccttcctt attgattgac ttttcagaca gacatacaat aacaacatcc
     1621 gaatcacgga tcgccttcgg aatttccacc tcccattttt gtcctgcaat tagactttca
     1681 gcatccaacc acgcatctac cccatcagca acaagacgtt tatacagatc gagcacagca
     1741 ggtttgtccg cagaagcgtg gcagagaaat actttgaggg ggcgtttggt ttcggacatt
     1801 ggtgggttta tcccgctaat ttgagttaat gagattatac ggcagaatct ctcaaaacta
     1861 tatgctatac tcgcgctatc cgactaaaaa ggagtggaaa atggataaat atttactacc
     1921 cgaagatgat ggcctgccag ctcgaacttt tggcaaatgg acaaccgcca aaacagatta
     1981 tcttcaaaga tatgtagatc tttttgaaac ctcaatgcgc ggtaaacctt ggtgcgctcg
     2041 atgttacatt gatctttact cgggaacggg caagtatcaa gttgagggac aaactggttt
     2101 tcatctgggc tccccattga tcggaataac aactaaatat cctttcactc attatttctt
     2161 tgcggatatt tcacaagaaa atattgacac actaaaacaa cgctgtgatc ccgcaatcca
     2221 attaagaaag aaattttact taggggatgc caatttactc gcccaagata ttgtcaacga
     2281 aattaaatcc attgaaagaa atcgttcaaa agaacaatgg gcatctttga acctagcatt
     2341 tctagatccc aacgggctgg aattagaatg gtcaaccatt gaaactcttg cttcagtaaa
     2401 caaaatggac ttaatcattt actattcaca aagcggtctg actaggaatt ttgaaaactg
     2461 catcaacgta gaaggcgata ccgatattga ccgttttttc ggggataggg agtggaggga
     2521 tatttacaaa acatctcacg gaaaaagtgc tggattgcac aggaaattga tcgatcacta
     2581 caagcacaag ttgtccactc ttggttatat ggatgtaaaa gatgttgaag atggagcgga
     2641 gccattgatg aaaaactcca aagacgcgcc gctatatcgc cttatttttg ccagcaaaca
     2701 cgcaagaggg catgacttct ggaacaagat cataaaaaag gatgcttttg ggcaagaaaa
     2761 actattctaa aaaatagaaa attcgtgcta ttattgtgta aacaaacttt acaagtagat
     2821 tcttcaaagg aggaagaatg gctgaacatt caggaatcga gtggacagaa tccacttgga
     2881 atcctttaac tggctgtacc aagatcagcc ctggctgtaa acattgctac gccgagcgca
     2941 tggcaaaacg cttgcaggcc atggggcaac ccaattatgt caacggcttc aaactcactc
     3001 tgcacgaaaa cgcaatggaa aaaccgctcg aatggaaaaa gccacagatc atcttcgtca
     3061 actccatgag cgatttgttc cataaagatg tacctgtcga attcgtgctt gaaatcttcg
     3121 atgtcatgcg ccgcgcacac tggcacactt tccaagtcct caccaaacgc gccgaacggc
     3181 tggaagaact cagtcccaaa attgattggc ctgaaaatgt gtggatggga gtcagcgtgg
     3241 aaaatcagga ttacacctac cgcattgacc acctgcgcag aacaggcgca aaaacaaaat
     3301 tcctgtcatt ggagccgttg ttggggccgc tgcacaaaat gaatctcaag ggaatcaact
     3361 gggccatcgt gggcggcgaa tctgggccag gggcacgtcc gcttgaccct gcgtgggtga
     3421 cggatattcg agatcagtgt ttgagagcta aagtcccatt tttcttcaag cagtggggcg
     3481 gcgtgcagaa gaaaaaagca ggcagagtcc ttgaaggcag aacgtgggac gagatgccgg
     3541 tcaacttggt gagagcgtaa atgaaaagcc agtccactac ggggctggct ttttttgatt
     3601 tcaaagctat ctctttcaat tctcatgcaa cacggatcaa ctcccccatc acacgttcct
     3661 tgatataggg tttcaaactg tcgg
//
LOCUS       DAOQJE010000270         3671 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_15807_length_3671_cov_5.944967, whole genome shotgun sequence.
ACCESSION   DAOQJE010000270 DAOQJE010000000
VERSION     DAOQJE010000270.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3671)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3671)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3671
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15807_length_3671_cov_5.944967"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..466
                     /locus_tag="PLT08_08255"
     CDS             <1..466
                     /locus_tag="PLT08_08255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE04495.1"
                     /translation="IDFGRGPSKLVTMGWGDVSTAYHSTGIPNVTVYMSFPKSMISVM
                     KFTKVAGPLLYSRAARNFIKWLIGIFLAPGPSREKNKTGFSLLIAEVTDGTKTVRAKL
                     RTPEAYYLTALTSVEIMKRILSNELKPGFQTPSKMYGADFILQFNGVEREDL"
     gene            606..1742
                     /locus_tag="PLT08_08260"
     CDS             606..1742
                     /locus_tag="PLT08_08260"
                     /EC_number="2.5.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010081119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase"
                     /protein_id="HNE04496.1"
                     /translation="MKFETLAIHAGQEPDPNNGAVMTPVYLTSTYKQDGIGKPRQGYE
                     YSRTLNPTRKALQDCLAELESGNWGLAFASGMAATDTVLRMLGQGDHVVAGNDVYGGT
                     FRLFDKILRRFGLDFTFADTTDPENLAEALTPSTRIVWLETPTNPLLAVSDIRAVAEV
                     VKNHPNKPLLVVDNTFATPYLQRPLELGADLVVHSMTKYLGGHSDVVGGAIIGKDKEL
                     GEKLAYLQNAIGGVQGPMDSFLVLRGIKTLPIRMDRHAQNAEKIVAFLEKQKNVKRLI
                     YPFHESHPQVQIAKRQMKNGGGMISFIMKDGRDAAVKVAESTKLFTLAESLGGVESLI
                     EVPAAMTHLSTQGSTLEVDPGLVRLSVGIENVDDLIADLEQALR"
     gene            complement(1773..3020)
                     /locus_tag="PLT08_08265"
     CDS             complement(1773..3020)
                     /locus_tag="PLT08_08265"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HNE04497.1"
                     /translation="MNEKMDSSVFLKIPLFKDLPKAELYSLVSELPVEKYQAGDYIFH
                     EGEPGNSLYVVQKGKIDVVLAGGTNDEMLLRTCGPGEYVGEMSLILKQGKRTAAVRVR
                     EDSQLWMMTRDKFNECLQRWPHLAYAMVEIISERLDSSNEAAFNDLVKKNQMLQKAYD
                     DLKAAQAQLIEKERLERELQLAADIQLSILPDVLPDTEEFGFGARILPARQVGGDFYD
                     VFQLKDDRIGVLIGDVADKGVPSALFMARAHALIMAEADIGATPAEVMNLVNSHITRL
                     QKSTQFVTVLYGILDLKTRIFSYARAGHEPPLVLHTDGSVERIPHSPGMAIGLWEPVT
                     LDEKTVKLSSGDTLLLYTDGMTDCRDPQGDAFGLERIKDKLGELSKQSAQQICNTLLE
                     TLQNHQNGAKQDDDVTLVAVKAK"
     gene            complement(3020..3655)
                     /locus_tag="PLT08_08270"
     CDS             complement(3020..3655)
                     /locus_tag="PLT08_08270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HNE04498.1"
                     /translation="MAIAGNIGAGKTTLTERIGARLGWWTGYESVADNPYLSDFYGDM
                     RSWSFHLQVFFLGHRADQYLEAARDARSAILDRSIYEDAHIFARALHHLGNLNERDYL
                     SYRKLFELVVNGLPRPDLLIHLKAPVPVLMDRIRRRARNMETGISAEYLTLLESFYDE
                     WLGSFDLCPVLTIQTDDLDYVNYPQHLDQVAQRIQDKLAGREMMDLRGDTK"
BASE COUNT          960 a          946 c          890 g          875 t
ORIGIN      
        1 tatagatttc gggcgcggac cttcgaaact tgtgacaatg ggctggggcg atgtcagcac
       61 agcatatcac agcacgggca tcccgaacgt gacggtgtac atgagctttc caaaatccat
      121 gatctcggtg atgaaattta ccaaggttgc aggtccgctg ctttattcac gagcggcaag
      181 aaacttcatc aaatggttga tcggaatatt ccttgcgccg ggtccttcac gcgaaaagaa
      241 caaaaccggt ttctcattgc tgatcgctga agtgaccgac ggaacaaaaa ctgtccgcgc
      301 aaagttgaga acaccagaag cgtattacct gacagcactg acttcggttg agatcatgaa
      361 gcgcattcta tcaaatgaac tcaagcccgg ctttcagacg ccaagcaaaa tgtatggtgc
      421 agattttatt ttgcaattca atggggttga gcgcgaagac ttgtaggttc aacaaaatgt
      481 caccctgagc gacatcgaag ggtttctact acaatgtaag agactcttcg gtcgcacaga
      541 acgctccctc agagtgacat gaattttctc ttttataatc acaaaaaccg accaaaggag
      601 catgaatgaa atttgaaacc ctcgccattc acgcagggca ggaacccgac ccgaacaacg
      661 gcgccgtgat gacgcctgtg tatctcacgt ccacatataa acaagacggc atcggcaaac
      721 cgcgtcaggg atatgagtat tcgcgcacgc tcaaccccac tcggaaggcg ttacaagatt
      781 gtcttgctga attggaaagt ggaaactggg gacttgcttt tgcctcaggc atggcagcca
      841 ccgatactgt cttgcgcatg ttgggacaag gtgaccatgt cgtcgcgggc aatgatgtgt
      901 acggcggtac gttccgcctg tttgacaaga tcctgcgccg cttcggtctg gactttacat
      961 ttgccgacac gaccgatcct gagaacctcg ctgaagcctt aaccccgtcc acgcgcatcg
     1021 tgtggttgga gactcccacc aatcctctgc tggcagtgtc tgatattcgt gccgttgcag
     1081 aagtagtgaa gaatcacccg aacaaacctt tattagttgt ggataacacg ttcgctacac
     1141 cgtacctcca acgcccactt gagctgggcg cggatttggt cgtccattcg atgacgaaat
     1201 atctcggcgg tcactcggat gtggtcggcg gcgcgatcat tggtaaagac aaagaacttg
     1261 gtgagaagtt ggcatatcta cagaatgcca tcggcggtgt gcaaggaccg atggactcct
     1321 tccttgtgtt gcgtggaatc aagaccctgc ccatccgcat ggatagacac gcccaaaacg
     1381 ccgagaagat cgtcgcgttc ttagagaagc agaaaaacgt caaacggctg atctacccat
     1441 ttcatgagag tcacccacag gtccagatag cgaagcgaca gatgaagaat ggcggcggca
     1501 tgatctcgtt catcatgaaa gatgggcgtg atgcagcggt caaagtcgca gaatccacca
     1561 aattattcac actggcagaa tcgctcggcg gcgtggagtc gctcatcgaa gtccctgcgg
     1621 cgatgacaca tctctccacc caaggttcca cgctcgaagt ggaccccggc ttggtacgtc
     1681 tctccgtggg catcgaaaac gtggacgatc tgatcgcaga ccttgaacaa gccctacgct
     1741 aaaacgaaga cctcccttgc gggaggtctt ttttatttcg ctttcacggc aaccaaagtc
     1801 acatcgtcat cttgcttggc accattctgg tgattctgta gggtttcaag caatgtatta
     1861 caaatctgct gtgcgctttg cttcgaaagt tcgccgagtt tatccttgat gcgttccaag
     1921 ccaaatgcgt caccttgtgg gtcgcggcag tcggtcatgc cgtcggtata taagagcaag
     1981 gtgtcgccag aagataactt gaccgtcttc tcgtccaacg tgaccggctc ccataacccg
     2041 atcgccatgc cggggctatg tggaatacgc tccacgcttc catcggtatg caataccaac
     2101 ggcggctcat gcccagcacg cgcatatgaa aagatgcgag tcttcaaatc caaaatacca
     2161 tagagcacgg tcacaaactg cgtggatttt tgcaagcgcg tgatgtgcga attgaccaag
     2221 ttcatcacct cggcaggagt cgcaccaata tccgcctctg ccatgatgag cgcatgggca
     2281 cgtgccatga acagcgcaga aggcacacct ttatcagcta catcaccgat caaaacccca
     2341 atgcggtcat ccttgagttg gaagacatca taaaaatcgc cgcctacctg acgggcaggc
     2401 aaaatccgtg cgccgaaacc aaactcttcg gtatcgggca aaacgtctgg gagaatgcta
     2461 agctgaatat cagctgccaa ttgaagttca cgttcgaggc gttctttttc gattaactgg
     2521 gcttgggctg ctttcaagtc atcataggct ttttgcagca tctgattctt tttcacaaga
     2581 tcgttaaaag ctgcttcgtt tgaagagtcc aaacgttcgc tgatgatctc aaccatggca
     2641 tatgccaaat gaggccagcg ttgcagacat tcattgaact tatcgcgggt catcatccaa
     2701 agctggctat cttcccgcac gcggacagcg gctgtgcgct tgccttgttt gagaatgaga
     2761 ctcatctcac ccacatattc gccgggtcca caggtgcgta gaagcatttc atcgttcgtg
     2821 ccgcccgcca gaaccacgtc gatcttgcct ttttgaacca cgtataaact attgcccggc
     2881 tcaccttcat ggaagatgta atcgcccgct tgatattttt ctaccggcag ttcgctgacc
     2941 aaggaataca actccgcctt gggcaggtct ttgaacagtg gaatctttag aaagacggaa
     3001 ctatccatct tttcattcat tatttggtat ctccacgcaa atccatcatt tctctacctg
     3061 ccaatttatc ttgaatacgt tgtgcaacct ggtcaagatg ttgaggataa tttacataat
     3121 cgaggtcatc tgtttggatg gtaagcacag gacaaaggtc gaaagagccg agccattcgt
     3181 catagaatga ctcgagtaag gtaaggtatt cagcagaaat gccggtttcc atgttacgtg
     3241 cacggcggcg aatgcggtcc atcaagactg gaactggcgc tttgaggtga atcaacaggt
     3301 ctgggcgtgg aagtccattc acgaccaatt caaacaactt gcgatacgaa agataatccc
     3361 gttcgttcaa gtttcctaaa tgatgcaagg cacgtgcaaa aatatgagca tcttcataaa
     3421 tactacggtc aagaatcgca gaacgagcat ctcgtgccgc ctcaaggtat tgatcagcac
     3481 gatgccccaa aaagaaaact tgcaagtgaa atgaccacga ccgcatatct ccataaaaat
     3541 cagagagata tgggttatca gctacagatt catatccggt ccaccagcca agccgtgcac
     3601 caatacgttc tgtcaacgta gttttgcctg caccaatgtt gccggcaatg gcaatgtggc
     3661 ggagttgctt t
//
LOCUS       DAOQJE010000271         3652 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_15949_length_3652_cov_6.386155, whole genome shotgun sequence.
ACCESSION   DAOQJE010000271 DAOQJE010000000
VERSION     DAOQJE010000271.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3652)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3652)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3652
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15949_length_3652_cov_6.386155"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..694)
                     /locus_tag="PLT08_08275"
     CDS             complement(<1..694)
                     /locus_tag="PLT08_08275"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF018076.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HNE04499.1"
                     /translation="MNWLEVSLTVNGELAESVADVLARFAPNGVMTEQGVRFVNDEDE
                     GTATGPITVRAYLEVNDQLEETRQKIEESLYYLGMITPVPTPTYKQIADQNWMEAWKQ
                     HYKPILIGEGLLILPAWLDNPDPKRIPIKIDPGMAFGTGTHPTTQLCLELMERSVNSN
                     WRLVIDVGCGSGILSIAALKRGAEKVLGVDIDIESVKNSRENADLNGIGEELTLGQGS
                     VTEVLAGQFAFKS"
     gene            complement(698..1177)
                     /locus_tag="PLT08_08280"
     CDS             complement(698..1177)
                     /locus_tag="PLT08_08280"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HNE04500.1"
                     /translation="MESRKYPVHIVAAGGFVINSENQVLMLKSPRYDDWEFPGGQIEE
                     GEIIPKGLEREVLEETGIVVRVKNLIGIYSNIGNPPIVNMDFLCEYISGEPKTSNESI
                     DVKWVDRNEVLNLVERKVIRTRLQNMLEFSGEINYLAYSVNPNIKDLSYQEFENRKI"
     gene            complement(1180..1563)
                     /gene="crcB"
                     /locus_tag="PLT08_08285"
     CDS             complement(1180..1563)
                     /gene="crcB"
                     /locus_tag="PLT08_08285"
                     /inference="COORDINATES: protein motif:HMM:TIGR00494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:1903425 - fluoride transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:1903424 - fluoride transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fluoride efflux transporter CrcB"
                     /protein_id="HNE04501.1"
                     /translation="MTNILLVGAGGFIGSVLRYLVSGYIQQSANRMDFPFGTLAVNII
                     GCFIIGLLAQFGDKYGTFSNESRAFLVIGVLGGFTTFSSFGNDTVNLIRQDFVMNAVA
                     NVGANVIIGLLAVWLGRTVGQLIWR"
     gene            complement(1560..2105)
                     /locus_tag="PLT08_08290"
     CDS             complement(1560..2105)
                     /locus_tag="PLT08_08290"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HNE04502.1"
                     /translation="MDESLLVFVLTADGELAVSGLSSAVVYNDSPMLKPLFRLWRLLP
                     MWVHVIVTRITQPKFNAGVSSLVFDEHGRVLLFKHTYRKYEWGIPGGALEYREQPIEA
                     VVREFYEETSMQIEVIKLLRVASAREFPHLGITYLCKITGGEFKPSHEISEMKYFDVN
                     DLPIMLFAEKDLIRWAVEESK"
     gene            complement(2138..3262)
                     /gene="dnaJ"
                     /locus_tag="PLT08_08295"
     CDS             complement(2138..3262)
                     /gene="dnaJ"
                     /locus_tag="PLT08_08295"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HNE04503.1"
                     /translation="MSNQDYYDILGVGKGASDDEIKAAFRKLARQYHPDVNKEEHAEE
                     KFKEINEAYGVLSDSEKRARYDRFGKAGVSGAGGQGYQDFSGFGDIFEDLFNGFGFST
                     GGGRSRRSPRRGRDLQMQVSLTFEEAVFGVEKEIEFSRDETCSRCSGKGAEPGTTPLR
                     CSTCNGQGEVRQVRQTFLGQMVQTAPCPTCNGRGETIASPCTTCRGNGLERKKVKKKV
                     SIPAGVDMGTQIRLNGEGAPGENGGPQGSLFLVLDVKPHKFFKRRDQDVILNLDINIA
                     QAVLGAEVTIPTLDGEDRLKIPSGTQPGKVFHVKGKGVPHVRNKNQRGDEIVIVNVAV
                     PTKLSKEQKELFEKLAESLGTTVKPQEKGFLDWLNDAFGG"
     gene            complement(3369..>3652)
                     /locus_tag="PLT08_08300"
     CDS             complement(3369..>3652)
                     /locus_tag="PLT08_08300"
                     /inference="COORDINATES: protein motif:HMM:NF012242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Hsp70 family protein"
                     /protein_id="HNE04504.1"
                     /translation="ADNTVYAGEKALRDLGDKVPAEIKAEVEAKSAEVKEAAKGEDVE
                     KIKAAVEALGQTIQKIGASVYEQGQPSAGGEAGPSSDAGPDVVDGEVKE"
BASE COUNT          874 a         1083 c          768 g          927 t
ORIGIN      
        1 cggacttgaa cgcaaactgc cccgccagca cctcggtcac ggaaccctgc ccgagggtca
       61 actcctcccc tattccattc aagtctgcat tctcacggga gttttttacg gactcgatgt
      121 caatgtcaac ccccaatacc ttttctgcgc cacgtttcaa cgccgcaatt gaaagaatac
      181 ctgagccaca ccccacatca attaccaatc tccaattact atttaccgat ctctccatca
      241 actccaaaca taattgcgtc gtcggatgag tacctgtgcc aaacgccatg ccggggtcga
      301 tcttgatcgg gattcgtttt gggtcggggt tgtccaacca ggcagggagg atgaggagcc
      361 cttcaccgat gagaatcggc ttgtagtgtt gcttccaggc ttccatccag ttttgatccg
      421 cgatctgctt gtaggtggga gtgggtaccg gtgtgatcat ccccaaataa tagagcgatt
      481 cctctatttt ctgtctcgtc tcttcgagtt gatcgttgac ctccaaatat gctcggacgg
      541 tgatgggacc cgtggcagtg ccctcgtctt catcgttcac aaagcggaca ccctgctcgg
      601 tcatcacgcc gttgggcgca aaacgtgcca gcacatcggc aacagattcg gcgagttcgc
      661 cgttgacggt caaagaaact tcaagccagt tcatgttcta tattttccta ttctcgaatt
      721 cttgataact caggtctttg atatttgggt tcacactata ggcgagataa ttaatttcgc
      781 cagaaaattc aagcatattt tgcaatcgtg tgcgaatgac ttttcgctct accaagttta
      841 acacttcatt tcgatcaacc cattttacgt caatgctttc gttgctggtt ttgggttcac
      901 ccgaaatata ttcgcacaga aaatccatgt tgacgattgg cgggttacca atgttcgaat
      961 agatccctat gaggttttta actctgacta caattcctgt ttcttccaac acttcccgtt
     1021 ctaaaccttt aggaatgatc tcgccttctt caatctgccc gccaggaaat tcccaatcgt
     1081 cgtagcgtgg acttttcagc atcaaaacct gattctcgct gttgatgaca aatccgcctg
     1141 ccgccacgat atgaactgga tattttctac tttccacact tatctccaga ttaattgtcc
     1201 aacggttcga cccagccaaa ccgcgagtag tccgattatc acattcgcgc caacatttgc
     1261 gacggcattc atgacaaagt cttgccttat caagttgacc gtgtcattgc caaaggacga
     1321 aaaggtggtg aagccgccta agacgccaat gaccaaaaag gcacgagact catttgagaa
     1381 cgtgccatat ttatcgccaa actgcgccag cagaccaatg ataaagcagc cgatgatatt
     1441 gaccgccaat gtaccgaacg gaaagtccat tcgattcgca gattgctgaa tatagccgct
     1501 gaccaaataa cgcaagaccg agccgataaa tccgcctgcg ccgacaagaa gaatgtttgt
     1561 cacttgcttt cctccactgc ccagcggatc aaatccttct ccgcgaacag catgatgggc
     1621 aagtcgttca catcgaagta tttcatctcc gaaatctcat gcgacggttt gaactcaccg
     1681 cccgttattt tacacaggta ggtgattccc aaatgcggaa attccctcgc acttgccacc
     1741 cgtaaaagtt taatcacctc gatctgcatg ctcgtctctt cgtaaaactc acggactacc
     1801 gcttcaatcg gctgctccct atattccaac gccccgccgg gaatccccca ctcatacttg
     1861 cggtaggtgt gtttgaaaag taacactctg ccatgctcgt caaagaccaa cgaagagaca
     1921 cccgcattga attttggttg agtaattctg gtaacaataa catgaaccca catgggtagc
     1981 agacgccaaa gacgaaatag gggtttaagc ataggcgagt cattataaac gaccgcagat
     2041 gatagaccgc tgaccgccaa ttcaccatct gccgtcaaaa caaaaacgag cagactttca
     2101 tccactcgtt tttaattacc aatctccaat taccgcccta tcccccaaac gcatcattca
     2161 accaatccag gaaccccttc tcctgcggct tgacggtcgt gcccaaactc tctgccaatt
     2221 tctcaaagag ttctttctgt tccttcgaca acttggttgg gacagccaca ttgacgatca
     2281 caatttcatc gccgcgctga ttcttattgc ggacatgcgg cacgcccttg cccttcacat
     2341 gaaagacttt tccgggctgt gtgccagagg gaatcttcaa tctatcttcg ccatcaagag
     2401 tcgggatagt tacttccgca cctaagaccg cttgtgcgat attgatatcg aggttcagaa
     2461 ttacatcctg atcgcggcgc ttgaagaact tatgcggctt cacgtccaac acgaggaaca
     2521 aactgccctg cggaccgccg ttctcgccgg gtgccccttc gccattcaag cggatctgcg
     2581 tgcccatgtc cacacctgcg gggatggata ctttcttctt gaccttcttg cgttccaagc
     2641 cgttgccgcg acaagtggta cacggcgaag caatcgtctc accgcgacca ttacaggttg
     2701 ggcatggagc cgtctgcacc atctgaccta aaaaggtctg acgcacctga cgtacttcgc
     2761 cctgcccatt acaagtggaa catcttaacg gagttgtccc cggctcagca cctttgccac
     2821 tacaacgtga acaagtctca tctcgtgaga actcgatctc tttctcaacg ccgaagaccg
     2881 cttcctcaaa ggtaagcgaa acctgcattt gtagatcccg cccacggcgc ggagagcggc
     2941 gcgaacgtcc gcccccggtt gagaatccaa atccattgaa cagatcttca aagatatcgc
     3001 cgaagccaga gaaatcctga tacccctgcc cgcccgcgcc gctgacaccc gccttgccaa
     3061 aacggtcata gcgcgcgcgc ttctcagaat ccgaaagcac accatacgcc tcgttgatct
     3121 ctttgaactt ctcttcggcg tgttcttcct tgttcacatc gggatgatac tgacgcgcaa
     3181 gtttacggaa cgcggctttg atctcatcat cgctcgcgcc cttgcccacg cccagaatat
     3241 cgtaatagtc ttggttgctc atatctgctt acgtcattgc gagggcgctc ttgctctttg
     3301 cccgaagcaa tctcattatg aatagaagat tgcttcgtcg ggaagaacac cctcctcgca
     3361 atgacatgtt attcctttac ttccccgtcc accacatcag gtccagcatc ggatgaagga
     3421 cccgcttcac ccccagccga cggctgaccc tgctcataaa cactggcacc aatcttttgg
     3481 atggtctgcc ccaaggcttc gacagcggct ttgatcttct ccacatcttc gcctttcgcc
     3541 gcttccttga cctcggcgct cttcgcctcg acctcagcct tgatctccgc cggaacctta
     3601 tcgcccaagt cgcgcaacgc cttctcaccc gcatagacgg tgttatcggc at
//
LOCUS       DAOQJE010000272         3651 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_15964_length_3651_cov_5.524472, whole genome shotgun sequence.
ACCESSION   DAOQJE010000272 DAOQJE010000000
VERSION     DAOQJE010000272.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3651)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3651)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3651
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15964_length_3651_cov_5.524472"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..229)
                     /locus_tag="PLT08_08305"
     CDS             complement(<1..229)
                     /locus_tag="PLT08_08305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE04505.1"
                     /translation="MLKLTRPLHLFLAALTYLLGASIPSYLGKPFLLAPFLLGLAFVL
                     LMQTSMSFLREVFRPHNEPLVEDETPQKKETL"
     gene            complement(231..896)
                     /locus_tag="PLT08_08310"
     CDS             complement(231..896)
                     /locus_tag="PLT08_08310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE04506.1"
                     /translation="MFVLLPYYVLLIVTSVFEKYFNSNFLLKDFISFFIEFFLLIFLE
                     IFTTVLVCKHTIFSSHEYPFWTMLKHYYERGLFLFVSTLFALILFGVVLAIFAPAIGK
                     FLYTLSTISCMFICTLSLQYMVVYNKDSIRDSLKGGIASLYKDFIFYFLVLLVGILIN
                     KFPSLLFPSSWLFLPILPIAEIEFILNPVAWLSWLIYPVLNSLQFISITLAFIYKHNK
                     WNS"
     gene            complement(961..2070)
                     /locus_tag="PLT08_08315"
     CDS             complement(961..2070)
                     /locus_tag="PLT08_08315"
                     /inference="COORDINATES: protein motif:HMM:NF012382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HNE04507.1"
                     /translation="MELTPFKLERYFAKYEFNTEYLLCSSDCESMSVADLLALEAGAM
                     ERFHKHWLGYTESQGSPSLRKEIAGMYATTQAEDILVFTGANEAIYLFMMASLKENDH
                     VIVHSPHYQALSEIAKGIGCMVSPWRAREENGWALDLDELRHLMRSSTKAIIVNVPHN
                     PTGYLMPRADFDALNQFAKENKLLLFSDEVYRESEYDSADRLPAAVDYGPHAVSLGVT
                     SKTYGLAGLRIGWVATKNRDLYAKIASLKDYTTICNSAPSEFLAEVAIRNRATLANRN
                     LEIIKNNLAIVDNLFSRHSSLFTWHRPRAGSMGFPKLLKGDVEAFCDDLVKKAGVLLL
                     PGSVYDDAHNHFRLGLGRKNLPQAAERLEDYLSER"
     gene            complement(2104..2574)
                     /locus_tag="PLT08_08320"
     CDS             complement(2104..2574)
                     /locus_tag="PLT08_08320"
                     /inference="COORDINATES: protein motif:HMM:NF023144.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HNE04508.1"
                     /translation="MSEIAELTEKLKTEGERMVAFFSELTDDQWVNEVYTEGTTWSIR
                     NVLSHFVTSERGLVKLFERIRATGEGASDDFSIDRYNASQQEKTKDLEPAELLEQYKQ
                     VRADSVQWVSGLKDEDLEIKGRHPFLGETVIREMIKMLYLHNQLHYRDVKRSLK"
     gene            complement(2592..>3651)
                     /locus_tag="PLT08_08325"
     CDS             complement(2592..>3651)
                     /locus_tag="PLT08_08325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE04509.1"
                     /translation="SLMLLAVIVMRGLKRSISIIAIFGVVFLILGGSYFLWRWNYFGY
                     PLPNPFYKKGEGGWSWQTFDASMLNMLRLSLPVVLAFILGFRSNETLKTTLAYMIPIF
                     GFAAAFGLISDEMNYGARFQYATVPLMLMSWIPLVRGFKFDGLNQLQVRERAVYFVAL
                     FAVAAGVVYYSWFQNCSLALYQQACDRPYERDGRLEMARMLADYRGKGYVMAVTEAGL
                     LPYYSGWNAIDTWGLNDQFVAHNGSITVEYLDKYKPEIIMFHDYYSPLVPPKLTDANL
                     AQRWFSMTILLKTYAEENGYILAAVFGDSPYDTHYYYVRPGFEDSKRLVEQISKFRDY
                     FYPTTGKRSINYAMYAQP"
BASE COUNT         1218 a          829 c          716 g          888 t
ORIGIN      
        1 gaagagtctc ttttttctgc ggggtttcgt cttcgacaag aggttcatta tgcgggcgga
       61 acacctcgcg caagaatgac atgctggtct gcataagaag tacaaaagca agacccagca
      121 aaaacggagc aagcagaaac ggtttgccta aataagaagg aatacttgca ccgagaagat
      181 aggtcagcgc agcaagaaaa agatggagag gacgagttag ttttagcaat ttaagaattc
      241 catttattat gcttataaat aaatgctaag gttattgaaa taaactgaag agaatttaat
      301 acaggataaa ttagccagct tagccaagct acaggattaa gtataaattc aatttccgca
      361 ataggcagga ttggaagaaa cagccaactt gaagggaaaa gaagggacgg aaacttatta
      421 attaaaatac ctacaaggag aactaaaaaa taaaatataa aatctttata taaactagcg
      481 atcccaccct tcaacgagtc tcgaatagaa tccttattgt aaacaaccat gtattgaagg
      541 gataatgtgc aaataaacat acagctaatt gttgagaggg tatacaaaaa tttcccgata
      601 gcaggagcga aaatcgctaa aacaactcca aacagtatta aggcaaaaag agttgaaaca
      661 aataaaaaca accctctttc gtaataatgc ttcaacatag tccagaatgg atattcatga
      721 ctagaaaata tcgtgtgttt gcaaactaac actgtagtaa atatttcaag aaaaatcaaa
      781 agaaaaaact caataaaaaa acttataaaa tccttcagca agaaattaga attaaaatat
      841 ttttcaaaaa ccgatgtcac aatcagcaac acatagtaag gcaataaaac gaacaatttt
      901 tttcttaaga ataatatcga gtctaggtaa acagaataga tcaaataaat cattattaac
      961 ttatctttcg gaaagataat cttccaaccg ttccgccgcc tgcggaagat tttttcttcc
     1021 caaccctaag cggaaatgat tatgtgcatc atcataaacc gagccgggca gcaaaagaac
     1081 gccagccttt ttgacaaggt catcacaaaa agcttctaca tcccctttga gtaatttggg
     1141 aaagcccatc gaaccagccc tggggcgatg ccaagtaaaa agcgaagagt gacgagaaaa
     1201 tagattatca acaatagcta ggttattctt gatgatttct aaatttcgat tcgccaaagt
     1261 agcacgattc cggatcgcaa cctcggcgag aaattcgctg ggagctgagt tgcatatagt
     1321 ggtgtagtcc ttcaacgaag caatctttgc gtacaaatca cggtttttgg tggcgaccca
     1381 accaatccgc agacctgcca agccgtaagt tttggaagtc actccaagag aaacagcatg
     1441 aggtccataa tccacggcgg cagggaggcg atccgcagag tcgtattcag actcacgata
     1501 aacctcatcc gaaaaaagga gaagcttatt ttccttggca aattgattta aggcatcaaa
     1561 atcggcgcga ggcataagat agccagttgg attatgggga acattgacga taatcgcttt
     1621 ggtcgatgag cgcatgagat ggcgtaattc gtccaaatcc aacgcccagc cattctcttc
     1681 gcgagcgcgc cacgggctaa ccatacagcc gatacctttg gcgatttcac tcaacgcttg
     1741 ataatgtggc gaatggacaa ttacatggtc attctctttc aacgaagcca tcatgaataa
     1801 gtaaatcgcc tcatttgccc cggtaaagac taatatgtct tctgcctgcg tggtggcata
     1861 catgccagca atctctttcc gcaacgatgg gctcccctga gactctgtat accccaacca
     1921 atgcttatga aatcgctcca tagcacctgc ttctaaagcg agcaaatctg ctacagacat
     1981 cgactcgcaa tctgaagaac agagtaaata ttcagtatta aattcatatt tggcaaaata
     2041 acgttcaagc ttaaacgggg tcagttccat gaaagtctcc agatattcac aaaaagcatt
     2101 gagctatttc aatgaacgct taacatcacg ataatgaagt tgattatgca agtaaagcat
     2161 ctttatcatt tcacgaataa ccgtttcccc taaaaaagga tgtcgccctt taatttccaa
     2221 atcttcatct ttcaacccac ttacccattg caccgagtca gcgcgaacct gcttgtattg
     2281 ctccagcaac tcagcaggct ctaaatcttt tgtcttttct tgttgagaag cgttataacg
     2341 gtctattgag aaatcatccg aagcgccttc tcctgtggca cgaatgcgtt caaaaagctt
     2401 caccaatccg cgttcagaag tgacgaaatg cgaaagcaca tttcgaatgc tccatgttgt
     2461 cccttccgtg taaacctcgt tcacccactg gtcatctgtc aattcggaaa agaaggcaac
     2521 cattcgttcg ccctcggttt tgagcttttc agtaagttca gcaatctcag acacgggcat
     2581 ctcctataaa tctatggctg ggcgtacatc gcataattga tcgagcgttt cccagttgtc
     2641 gggtaaaaat aatcacgaaa ctttgatatc tgctccacta aacgcttgct atcttcaaag
     2701 ccggggcgca cataataata atgggtatca tacgggctat cgccaaacac tgccgcaaga
     2761 atataaccat tctcttcagc ataggttttc aacaaaatcg tcatgctaaa ccagcgttgc
     2821 gcaaggttgg catctgtcaa cttcggcggc accagcggcg agtagtaatc atggaacatg
     2881 atgatctccg gcttgtattt atccaaatac tccacggtaa tcgagccatt gtgcgcaaca
     2941 aattgatcgt tcagccccca tgtatcaatt gcattccagc cagaataata cggcaacaaa
     3001 cctgcctctg tcaccgccat tacataaccc tttccacgat agtccgctaa cattcttgcc
     3061 atttccaagc gcccatcacg ttcgtaaggg cgatcacatg cttgttgata taaagccaga
     3121 gaacaatttt ggaaccagga ataataaacc accccagcag ctacagcaaa cagtgcgacg
     3181 aagtacacgg ctctttcgcg gacctgaagc tggttcaacc catcgaactt gaatccacgc
     3241 acaagcggaa tccacgacat gagcatgagc ggcacggttg catactgaaa acgagcacca
     3301 taattcattt cgtctgagat caagccaaac gccgctgcaa acccaaagat gggaatcata
     3361 taagccaatg tagttttaag ggtttcgttt gagcgaaagc cgagaataaa tgccaacaca
     3421 acaggcaaag ataatctcag catatttagc atagacgcat caaacgtctg ccaagaccaa
     3481 ccaccttcac cctttttata aaatgggttt ggaagaggat agccaaaata attccaacgc
     3541 caaaggaagt atgaaccacc caatatcaag aaaacaaccc caaagatcgc gataatagat
     3601 atggaacgct taagtccgcg cattacgatg acagccagaa gcatgagtga a
//
LOCUS       DAOQJE010000273         3647 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_16002_length_3647_cov_3.446269, whole genome shotgun sequence.
ACCESSION   DAOQJE010000273 DAOQJE010000000
VERSION     DAOQJE010000273.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3647)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3647)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3647
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16002_length_3647_cov_3.446269"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1323
                     /locus_tag="PLT08_08330"
     CDS             <1..1323
                     /locus_tag="PLT08_08330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE04510.1"
                     /translation="PFVEQHAWGYTESDLGWSLAQIFAEGLKTSYTTSGLETNVKILG
                     PADKVPTIFGFNEDHLVHSAETVSEKINGQVVIYGVLSQDEYGDTVAQVKVYISPTNF
                     GEAQELISDSMMGDLSLGSFVLTGETANGTDLEAQNEDLRNRLAIFASLINFLGAYLG
                     DDFSSAQKYIEAAGDSILWNDPNGLEVIQLLQGNLELRRARVSIVQKDLEGTQASIDL
                     ARTYFGNARETALKNNKGQYARAYLGLAGAENLAAVAEANINGDPSAINTDALKETLA
                     LLEQAEQANYAPDSADVSVKANYGRAQVNLAYFAKTGNSDYLTEAKKYYQFVVEEYKR
                     TDNKRIVDYTALSHSGLGLIASQENQVTVAIENFTSAYQLTGNPSLKIQCLVNMGDIY
                     FANKMYAEALKVYQDALSRRNDLENAISAERIDLIEERINFIKNGGTL"
     gene            1320..2165
                     /locus_tag="PLT08_08335"
     CDS             1320..2165
                     /locus_tag="PLT08_08335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0070064 - proline-rich region binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HNE04511.1"
                     /translation="MNKTAPLLALFTFLSLVASCSTSQPVSNENESQSASIENSNAST
                     IPTVDINEPVINIPSTNETAPAGILEQINWAPSGGGDTTVTPTPNPGCVQECSVNIKD
                     SHIFLTNFNPNQKVRIVFYTRSDQTMTCMMFLIEYVTNSIVQVDESGNFSAKLSDTNK
                     PLSVVVLDYNTNEKIWGFEYQYTPCDISYYNACSGAPKQRLKIDEKAYVCTQKDSVKL
                     RSDAGKEYSEIKALIPGAEVTVIDGPLCADNWSWWQVRTESGFIGWMSEGGDEVDEYF
                     LCPLP"
     gene            2216..3073
                     /locus_tag="PLT08_08340"
     CDS             2216..3073
                     /locus_tag="PLT08_08340"
                     /inference="COORDINATES: protein motif:HMM:NF019844.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0070064 - proline-rich region binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HNE04512.1"
                     /translation="MNKTSQLLALFTLLTFLVSCSTPRQASNNDRQNGSSEISNNPAI
                     PVDSLNEPIINLPSTNEVAPAGILDQLAWTERGGGDASTPTPAPIPICSDCNIRMQNS
                     QVVLLTNFAPNQKIRIVFYKHSTERSSCGFPSANYLTNSEVQVDEQGNLSIQLNGADM
                     NVFVAVAFDANTGPKVWELSGRYGYEDCNVSVPSGSSCPGAPPQRLQVNKMAYVCTQS
                     DSVKLRSDAGKQYSEIKSLVPGADLTIIGGPICADNWSWWQVKTESGFIGWMSEGGDE
                     VDEYFLCPK"
     gene            3097..>3647
                     /locus_tag="PLT08_08345"
     CDS             3097..>3647
                     /locus_tag="PLT08_08345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE04513.1"
                     /translation="MEFRIAKTFEESLSRLANEEQKAAKITVTDLQLDPSAPGLSFHK
                     LDRAQDPNFASVRISRDLRLIVHQLHGNLLICYVGHHDNAYQWAERRKLEVHPTTGAA
                     QIVEVREVVQDVIIPNYIIEDRPKPRLFAKHSDEILLGYGVPPEWLEDVRNATEDTIL
                     YVVDHLPEEAAEAGLESLQQAAG"
BASE COUNT         1070 a          862 c          814 g          901 t
ORIGIN      
        1 ccatttgtcg aacaacatgc ctgggggtat accgaaagtg accttggctg gagtcttgct
       61 caaatctttg cagaaggttt gaaaacatcc tatacaacat caggactcga aaccaatgtc
      121 aaaatccttg gtcctgccga taaggttcca accatttttg ggttcaatga agatcatttg
      181 gttcattccg ctgaaacggt ctccgaaaag atcaatggtc aagtggtgat ctatggagtc
      241 ctttcacaag atgaatatgg ggataccgtg gcgcaggtca aggtgtacat ctcgccaacg
      301 aattttggcg aagcgcagga actgatctcc gactccatga tgggagatct atcgctcggt
      361 tcttttgtgc tgacaggaga aacggcgaat ggaaccgatc tagaggcgca aaacgaagat
      421 ttgcgtaacc gccttgctat tttcgcctcc ctcattaatt ttctgggagc ctaccttggg
      481 gatgatttct ccagtgccca gaagtacatt gaagccgcag gtgattcaat tctttggaat
      541 gaccccaatg gcttggaagt catccaactg ctgcaaggca acctggaact tcgccgcgcc
      601 agagtgtcga tcgttcaaaa agacctggaa ggcacgcagg catccataga tcttgcgcgt
      661 acttactttg gaaacgcacg cgagactgcc ttgaaaaaca acaaagggca atacgcacgg
      721 gcatatcttg ggctggctgg ggcggaaaac ctagctgcag tagcagaggc aaatatcaat
      781 ggggatcctt ccgcgatcaa taccgatgcg ctcaaagaaa cactcgctct cctcgaacag
      841 gcagaacagg caaattatgc ccccgactct gcagatgtaa gtgtcaaagc caactatggc
      901 agggcacagg tcaatctggc atattttgca aaaacaggca acagtgatta cctgacagaa
      961 gcaaaaaaat attatcaatt cgtagtggaa gagtataaaa ggacagacaa taaacggatc
     1021 gtagactaca ccgccctgtc ccattcaggg ttgggtctta tcgcctctca agaaaaccaa
     1081 gtgactgttg cgatcgagaa tttcacatct gcctatcaac tgaccggcaa tcccagcctc
     1141 aaaatccaat gtcttgtcaa catgggtgat atttattttg ctaacaaaat gtatgcagaa
     1201 gcattaaagg tatatcaaga cgcgctaagt cgaaggaacg atcttgaaaa tgcgatctcg
     1261 gcggagagaa tcgatctcat tgaagagaga attaacttta tcaagaatgg aggaacgcta
     1321 tgaataaaac agctccactt ctcgctctat ttacgttctt gtctcttgta gcatcctgtt
     1381 caacatcaca gccggtaagt aatgaaaatg aaagccaaag tgcttcaatt gaaaatagca
     1441 atgcttccac cattcccact gttgacatca atgaacctgt cataaatatc ccatcaacaa
     1501 acgagaccgc ccctgctggt attctcgaac aaatcaattg ggcgccaagc ggcggcggag
     1561 atacaacagt gacgccaact cctaatccgg gatgtgttca agaatgtagc gtcaatataa
     1621 aagactcaca tatatttctc accaatttca acccgaacca aaaagtgaga attgttttct
     1681 atactcgctc agatcagact atgacatgca tgatgtttct cattgaatac gtaacaaact
     1741 cgattgttca ggtagatgag agtggcaatt tttctgccaa acttagtgac accaataaac
     1801 ctttatctgt tgtagtgctt gattacaata cgaacgaaaa aatatggggt tttgaatacc
     1861 aatacacacc atgtgacatc tcttattaca atgcttgttc aggcgcgccg aaacagcgtt
     1921 tgaaaatcga cgaaaaggct tatgtatgca ctcaaaaaga ttctgtaaag ctcagatccg
     1981 atgccggtaa ggaatattct gaaataaaag ccctgatccc cggagccgaa gtgactgtga
     2041 tcgatggtcc tttatgtgca gacaattggt cgtggtggca agtcaggact gagtcaggct
     2101 ttattggctg gatgtcagag ggtggagacg aagtagacga gtatttcttg tgtccgcttc
     2161 cttaggaatc gcgaaactgc atgaaatcac cactacattg actgggagat tagccatgaa
     2221 caaaacatct caactactgg ctttgtttac cctcttaacc tttttagttt cctgttcaac
     2281 cccacggcaa gcaagtaaca atgaccgcca aaatggttca agtgaaatct caaacaaccc
     2341 agctattccc gttgatagtt tgaacgaacc cataatcaat ctaccatcaa cgaatgaagt
     2401 tgcccccgct ggtattcttg atcaacttgc atggacggaa cgcggtggtg gagatgcgtc
     2461 aactccaacg cccgcaccta ttccaatatg tagcgattgt aatattagaa tgcaaaattc
     2521 tcaggttgtg ctgctcacca attttgcgcc caatcaaaaa attagaattg ttttctacaa
     2581 acattccacc gaacgatctt catgcggatt tccatctgcc aattatttga cgaattcaga
     2641 ggttcaagta gatgagcagg gcaatttgtc aattcaacta aatggtgccg atatgaatgt
     2701 atttgttgct gttgcctttg atgcgaatac agggccaaag gtatgggaac tttcaggcag
     2761 atatggttac gaagactgta atgtatctgt tccctctgga agttcttgtc caggtgcgcc
     2821 gcctcaacgt ctacaagtaa ataaaatggc ttatgtatgt acccaaagcg attctgtaaa
     2881 gcttagatca gatgcaggga aacaatactc tgaaataaag tctcttgttc ccggagctga
     2941 tcttacaatc attggtggac caatatgtgc agataattgg tcttggtggc aagttaagac
     3001 cgagtcaggc tttattggct ggatgtcaga aggcggagac gaagtggatg aatatttcct
     3061 gtgccctaaa tagtagaaat tcaaagaaga ggacaaatgg aattccgtat cgctaaaacc
     3121 ttcgaagaaa gcctgagtcg ccttgccaat gaagaacaaa aagcggcgaa gatcaccgtc
     3181 accgacctgc agcttgatcc ctccgcgcct gggcttagct tccacaaact cgaccgcgcc
     3241 caagacccca acttcgcctc cgtccgcatc agccgcgacc tgcgtctaat cgtccatcaa
     3301 ttgcatggca atctgcttat ctgctatgtc ggtcatcacg ataatgctta ccaatgggca
     3361 gagcgccgca agctcgaagt gcatcccact accggcgctg cgcagatcgt cgaagtccgc
     3421 gaagtggtgc aggatgtcat catccccaat tacatcatcg aagaccgccc caaacctcgc
     3481 ctctttgcca aacattccga tgaaatcctg ctcggctacg gcgtcccacc cgaatggctg
     3541 gaggatgtcc gcaacgccac cgaagatacg attctttatg tagtagatca cttgccagaa
     3601 gaagctgccg aagccgggct ggaatctctt caacaagcag caggaca
//
LOCUS       DAOQJE010000274         3637 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_16058_length_3637_cov_5.769403, whole genome shotgun sequence.
ACCESSION   DAOQJE010000274 DAOQJE010000000
VERSION     DAOQJE010000274.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3637)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3637)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3637
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16058_length_3637_cov_5.769403"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2007)
                     /locus_tag="PLT08_08350"
     CDS             complement(<1..2007)
                     /locus_tag="PLT08_08350"
                     /inference="COORDINATES: protein motif:HMM:NF013434.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HNE04514.1"
                     /translation="MKTHAEIVVIGGGIYGAQVAYHLAKNGRKDVVIVEKGEVASGES
                     SHAAGLVTQFATSQAMLRFRMYSVQMYKELGLFDTVGSLRVASSKEQLLEMERSVSRA
                     KALGLDCEVISPEESKKYMPQISDQDLYGGIYLPGDGQLDPYTVTTSMARFAKELGVE
                     IYTNTRVTGIKVSAKGEVEAVVTDKGTIRCEIIVNAAGMWAPRIAAMAGLHVPTTPVD
                     HQHIALRAVPGHEFNDQTPCLRDPDNLVYMRQEKGGLVIGGYEPKPLPRWIDGTPWEH
                     GSKSFPGDFDQFEMLLEGAIRRLPFLDQAGIITLVRHPGAYTPDCQPLLGPMPGVKGF
                     WMMAGMSLNGYGGAGGMGKLMAEWIMDGEAPMDVYGYRATRFGNYYSDFNYAAERTVE
                     AVKYYYRLNFPHDESEAARPHRTSPVHYRLMENGAVFGEKFGWERVNYFNPGNEWRRM
                     GEDQREWGWTKPPYFERMRVEHIATRERVALFDLTSFGKIEVKGPGALPLLQRLTSSN
                     IDKPVGSAVYTQFLNRNGGIESDLTVTRLGEAYFWVITGSGFIANDHARILMNVNEAD
                     GEVSIRDITQEHACLALWGPKARDVLKKITSSDVSNEGHPYLTTKPIQINGVKVIAQR
                     VSYAGELGWELYIPNNRATMVWDMLVEAGKEFGMELGGYKVLDPL"
     gene            complement(2091..2879)
                     /locus_tag="PLT08_08355"
     CDS             complement(2091..2879)
                     /locus_tag="PLT08_08355"
                     /inference="COORDINATES: protein motif:HMM:NF012669.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HNE04515.1"
                     /translation="MGKPLIPAQRREKIQEYLGIHQIARTADLMDLLEASEATVRRDL
                     EWLEQKGILERTHGGAVLNQRVIFEQEYQLRLKNYPDEKKQIGEFAATLIEEGDIVFI
                     NSGTTATQVLQHVPRNPRITVFTNNVSALVDIGDPGFSLNLTGGEFQARSNSLSGRFA
                     LDNLNQVFANKAILGVDGISLKHGLTVPTNPEAEVVRKMIERTKGQVIIVADHSKWGA
                     VSNFHVASIEAMDKLVTDTGFSKQAKKELADYSIEVFLANNMKV"
     gene            3007..>3637
                     /locus_tag="PLT08_08360"
     CDS             3007..>3637
                     /locus_tag="PLT08_08360"
                     /inference="COORDINATES: protein motif:HMM:NF012780.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HNE04516.1"
                     /translation="MTKLVKDLMHKGVITCKPDATLGQVATLLDQHQVHALFVTDRDG
                     RIVGVISDFDLMTGEWLSSDPESLNTMRTLTASDLMTKPVDTIEAATPLTEAADLFMQ
                     KKVGRFLVLEHEKPVGTISLSDFVASLSERIKSKREKVGDVMSHAILVCRGKTPVLSA
                     AYAMTSAGWRSVLVVDLNGKVIGVVSGHDLLHLVKNGVDEKLTVRDVMHP"
BASE COUNT          926 a          981 c          797 g          933 t
ORIGIN      
        1 tagcgggtcc agcactttat agccgccgag ttccatgcca aattctttac cagcttcaac
       61 aagcatatcc cataccatgg tggcgcgatt gttcggaata tacaactccc agcccaattc
      121 gccagcatag cttactcgtt gtgcaatcac tttcacgccg ttaatttgaa tcggctttgt
      181 ggtcagataa ggatgccctt cattagacac gtcactgcta gtaatcttct ttaatacatc
      241 acgcgccttg ggtccccaca aggcaagaca ggcatgttcc tgcgtaatat cacggataga
      301 cacttcgccg tctgcttcat tcacgttcat caaaatccgc gcatggtcgt tggcaataaa
      361 accagaacct gtaataaccc agaaatatgc ttcacccaaa cgagtgaccg tcaaatcaga
      421 ctcaataccg ccattgcgat tcaagaattg ggtatacacc gcgctgccaa cgggcttatc
      481 aatattactc gacgttaagc gttgcaacaa aggcaatgca ccgggtcctt tgacttcgat
      541 cttgccaaaa gaggttaggt caaacagtgc cacacgctca cgggttgcaa tatgttccac
      601 tcgcatccgt tcgaagtatg gaggtttcgt ccacccccac tcgcgttgat cttcgcccat
      661 acgacgccac tcattacctg ggttgaaata attcacgcgc tcccagccaa acttctcgcc
      721 aaaaaccgcg ccattttcca tcaaacgata atgcaccggg ctggtgcgat gcggacgtgc
      781 cgcttccgat tcatcgtgcg ggaaattcaa acgatagtaa tactttacgg cttccaccgt
      841 tcgttcggcg gcatagttga aatcagaata gtaattgcca aagcgtgtgg cacgatagcc
      901 atacacatcc atcggtgctt caccatccat gatccattcc gccatcagtt tgcccatgcc
      961 acctgcgcca ccatatccat tgagcgacat gcccgccatc atccagaaac ctttcacacc
     1021 gggcatcggt ccgagcaagg gctggcaatc gggcgtgtac gcaccggggt gacgtactaa
     1081 cgtaatgatg cccgcctgat ccaaaaacgg aaggcgacga atcgcacctt caagcaacat
     1141 ttcaaattgg tcgaaatcac cggggaaaga tttactgcca tgttcccacg gcgtaccatc
     1201 gatccaacgt ggaagcggct tcggctcata cccaccgatc acaagccccc ctttttcctg
     1261 acgcatatac accaaattat ccgggtcacg caagcaaggc gtctgatcgt tgaactcatg
     1321 cccaggcaca gctcgcaacg caatatgttg atggtcaacc ggagtggtcg gaacgtgcag
     1381 acccgccata gcagcgatgc gcggcgccca cattcctgcg gcattgacaa tgatttcgca
     1441 tcgaatcgtg cccttatctg ttacaaccgc ctcaacctca cccttcgctg acactttgat
     1501 tccggtcacg cgggtgttgg tataaatctc cacgcctaat tctttagcaa aacgcgccat
     1561 cgatgtagtg acagtatacg gatcgagttg accgtcacca ggtaggtaaa tgccgccgta
     1621 cagatcttgg tccgaaattt gcggcatata ttttttggat tcttctggtg agataacttc
     1681 acaatccagt cccaacgcct tggcacggct cacgctgcgt tccatttcca acaattgttc
     1741 tttgcttgaa gccacgcgca aactaccaac tgtatcgaat aaaccaagtt ctttatacat
     1801 ctgcacgcta tacatacgga agcgtaacat tgcctgtgag gtggcaaact gcgtcaccaa
     1861 ccccgccgca tgcgaagact caccgctagc aacctcccct ttttcgacta tgacgacatc
     1921 cttgcgtccg ttcttggcaa ggtgataagc aacctgtgcc ccataaattc ccccaccaat
     1981 aacaacgatc tctgcatgag tcttcattca tcaactccgt agtttgattg tgatgtaaaa
     2041 caagaaaaca cgttcaaaac aaatgtctta aaacgtgttt tatatttgtg ttagaccttc
     2101 atgttatttg caagaaaaac ttcgatcgaa taatctgcca gttccttttt cgcttgttta
     2161 ctaaaaccag tgtcggtaac aagtttatcc atcgcttcga tacttgctac atgaaaatta
     2221 gaaaccgcac cccatttgct atggtctgcc acaataatga cctgtccctt cgtgcgttca
     2281 atcatcttgc ggacgacttc tgcttcaggg ttggtgggaa ccgtcagccc gtgttttagg
     2341 gagattccgt ctacgccgag aatcgcttta ttagcgaaga cttgattcaa attgtccagc
     2401 gcgaagcgac cggaaagaga gttggaacga gcctggaatt cgcctccggt caaattgaga
     2461 gaaaaacccg ggtcgccaat atccaccaag gcactgacgt tattcgtaaa aaccgtaatg
     2521 cgtggattcc tcggaacgtg ctgcaatact tgtgtagcag tggtaccgct gttgataaaa
     2581 acaatatcac cctcttcaat aagggtggcg gcaaactcac cgatttgttt cttttcgtcg
     2641 ggataattct tgagacggag ttgatactcc tgttcgaaga tcacacgttg atttaagacc
     2701 gcgcccccat gcgtgcgctc taggattcct ttttgttcca accattccaa gtcgcggcga
     2761 acggttgctt cagaagcttc aagcaaatcc atcaggtcgg cagtacgcgc gatttgatgg
     2821 atgccaagat actcttgaat cttttctcta cgttgggctg gaatgagggg tttacccatc
     2881 tgacggatta tataagaatc tgaaggaatt tgcaagtttt tgattttttg cttataatgg
     2941 atttgtaaca cagacctgac agcttaatct cacaaaacct gtcgggtctt cttttttgga
     3001 gactgcatga caaaacttgt caaagacctt atgcacaaag gcgtgatcac ttgcaaaccc
     3061 gatgcaaccc tgggtcaagt agctaccttg ctcgatcaac atcaagtgca tgccttgttc
     3121 gtcacagatc gcgatggtcg gattgtggga gtcatttcag actttgattt gatgacaggc
     3181 gaatggcttt cttcagaccc tgaaagcctc aacaccatgc ggacactcac cgcctcagac
     3241 ctaatgacca agcctgttga caccatcgaa gccgctactc ctctgacgga agccgccgac
     3301 cttttcatgc aaaaaaaggt tgggcggttt ttagttttag agcatgaaaa accagttggc
     3361 acgatctcgc tttcagattt tgttgccagc ctttccgagc ggatcaaatc taaacgcgag
     3421 aaagtcggcg atgtgatgtc gcatgccatt cttgtttgcc gtggcaagac accagttctt
     3481 tccgccgcct atgccatgac ttctgccggc tggcgctcgg tgctagttgt ggacttgaac
     3541 ggcaaagtca tcggtgtagt gagcggacat gatctcttgc atctcgtcaa gaacggcgtg
     3601 gatgaaaaat taaccgtccg cgatgtgatg caccctg
//
LOCUS       DAOQJE010000275         3631 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_16098_length_3631_cov_5.863255, whole genome shotgun sequence.
ACCESSION   DAOQJE010000275 DAOQJE010000000
VERSION     DAOQJE010000275.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3631)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3631)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3631
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16098_length_3631_cov_5.863255"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..613)
                     /locus_tag="PLT08_08365"
     CDS             complement(<1..613)
                     /locus_tag="PLT08_08365"
                     /inference="COORDINATES: protein motif:HMM:NF013429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HNE04517.1"
                     /translation="MALSFKFGTVGSPSGTPKKPGGTIGAIAFSKSIGLETLELGWVQ
                     SVRVSEETCALIKATRDEHNFALSVHAPYFINLNAEKEEWPKSRKRLMDAAHYGYLAG
                     VTDIIFHPGSYFGNDPAEVLKVAIPRLEGCVKELQKNGDKVTLRPETMGKSAMLGSFE
                     DALAMSKTVDMVQPCIDFAHLHARPGDGSMNSYDEWSRLLEKYG"
     gene            complement(657..1043)
                     /locus_tag="PLT08_08370"
     CDS             complement(657..1043)
                     /locus_tag="PLT08_08370"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013391.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HNE04518.1"
                     /translation="MKEFILSRIRSFRYAFQGWGYVLRTQRNAWIHSAIATAVFIVGL
                     WLQLPARDWAVIILTAALVFSAEFINTAIEAVVDLATEEHHPLAKIGKDVGAAAVLVA
                     ALAAVLIGLLILGPPLLQKIKSIYAF"
     gene            complement(1046..1516)
                     /gene="ybeY"
                     /locus_tag="PLT08_08375"
     CDS             complement(1046..1516)
                     /gene="ybeY"
                     /locus_tag="PLT08_08375"
                     /inference="COORDINATES: protein motif:HMM:TIGR00043.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - ribonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HNE04519.1"
                     /translation="MIHIDNQMDFRDTALLERAALVTLESVTPLNPDLGPDSAHADMS
                     IVLTDDRQLHELNLDYLGVDAPTDVLSFPAGEVDPETESLYLGDLAISIPRATQQAQN
                     GGHPVEAEAQLLVVHGTLHLLGYDHGTDEEKAVMWAEQAKVLERLGLSRIKIQE"
     gene            complement(1513..>3631)
                     /locus_tag="PLT08_08380"
     CDS             complement(1513..>3631)
                     /locus_tag="PLT08_08380"
                     /inference="COORDINATES: protein motif:HMM:NF019317.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="HDIG domain-containing protein"
                     /protein_id="HNE04520.1"
                     /translation="VRMTTRPPVPRRTRILQYLMIAVVALASFGMLISPLSLNPNQQT
                     YDIGEVTQSSIRSPRDIEYVSEIRTEEARKAAESAVQSVYSPPDPSITRAQIERLRTS
                     LLNISAIRDNKELTNEEMRQSLLSLSDLRLKDETIDYLLGISSTRWQSVQDEAVRVLE
                     QVMRRAIYEDRVEAAQSSISSSVSLTFNEQQTELVTELVSPFVIANSFFSQELTDAEK
                     LRAREAIQPIVQTYKAGETIVPAGEIITPADVEAFQQLGMIRPGQRWEDIAATAAIVL
                     LCAAFVPIYFYRKPRSVFLNDPKNLTIISLLFVVFLAGARLLAERTLAPYGYPIQAAG
                     LLLTALFSMEVGLVFAIPLAVLSAFGLPNSADLIPYYMFTSLIGLLALGPARRFWGFL
                     RAGIAISISGAAVLMAFRIPLITLDWLGILQYMGMIAFAGFSSASIALLLQYIFAQIL
                     GLTTALQLLDISRPDFPLLQFFLRNAPGTYQHSLQVANLAEQAAEKISADPLLTRVGA
                     LFHDIGKALNPNFFIENQVQGNLNAHNNANPEEVSATIIRHVTDGVQLAKKHRLPRRL
                     HDFILEHHGTLITHYQYNQAMEAVGGDISKVDIEKFRYPGPRPASRETALLMLADATE
                     ARARAERPTNDEEIRALVTSVIQTVQKYNQLDDTLLTLRDLRLITDSFVTTLRGTYHP
                     RIQYPKAAVPDQDTTLLVTRKEK"
BASE COUNT          952 a          879 c          959 g          841 t
ORIGIN      
        1 tgccatattt ttctagcaag cgagaccatt catcgtagct attcattgac ccatcaccag
       61 ggcgggcgtg gaggtgggca aagtcgatac aaggctgtac catatcaaca gttttagaca
      121 tggcaagcgc atcttcaaaa gaaccaagca tggcagattt gcccatcgtt tcagggcgta
      181 gggtcacttt gtcaccgttc ttttgcaatt ctttcacaca accttccaag cgcggaattg
      241 ccaccttcaa tacctcggca gggtcattgc caaaatacga gccagggtga aagataatat
      301 ctgttactcc agcaaggtaa ccataatgcg ccgcgtccat gaggcgctta cgcgatttgg
      361 gccattcttc cttctctgca ttcaagttga tgaaatatgg cgcatggacg ctcaatgcga
      421 aattatgttc gtctcttgtg gctttgatca atgcacaagt ctcttcgctc actcgcaccg
      481 attgcaccca gcccaattcc agcgtttcca aaccgatcga cttgctaaat gcaatcgccc
      541 ctatcgtccc gccgggtttc ttgggagtgc cagagggtga accaactgtg ccaaatttga
      601 aagagagtgc catgagagtc tccagaaaat agaaatgagt aacagtaatt cgtcaattag
      661 aaagcgtata tggatttgat tttttgcaac aacggcggac ccaaaatgag caagccaatg
      721 agaacagccg ccaacgccgc cactaacaca gccgccgcgc caacatcttt gccgatcttt
      781 gccagcggat gatgctcttc tgtagcaagg tccactacgg cttcgatggc ggtattgatg
      841 aactcagcag aaaaaaccaa tgccgccgta aggatgatca ccgcccaatc tcgtgccggg
      901 agttgaagcc acaaaccaac aatgaaaaca gccgtggcaa tagcgctatg aatccatgca
      961 ttgcgttgcg ttcgcagaac atagccccag ccttgaaaag catatcgaaa cgaacggatg
     1021 cgcgagagga taaattcctt cacgattatt cctgtatctt gatgcgagac aaaccgagtc
     1081 tttctaaaac tttggcttgc tctgcccaca tcacggcttt ttcttcatcc gtgccgtggt
     1141 cataaccaag caagtgtaac gtcccatgca ccacaagcag ctgtgcctcg gcttctacag
     1201 ggtgcccccc attctgcgct tgttgtgtgg cgcgcggaat ggagatagcc aggtcaccga
     1261 ggtagagtga ttcagtctcc gggtccactt cgccagcagg aaaggagagc acatctgtag
     1321 gggcatccac gccgagataa tcgagattca gttcgtgaag ttggcggtca tcggttagga
     1381 cgatggacat atctgcatgg gcagagtccg gtccaaggtc cgggttgaga ggcgtgacag
     1441 attcaagagt cacaagcgcc gcacgttcaa gcagtgcggt atcgcggaaa tccatttggt
     1501 tatcaatatg aatcattttt cttttctcgt aactaacagg gtcgtatcct ggtcgggtac
     1561 ggcggctttg ggatattgaa tgcgcggatg atatgtgccg cgcaaggtgg tgacgaaaga
     1621 atcagtgatg agacgcaagt cgcgcaaagt gaggagggta tcgtcgagct ggttatattt
     1681 ttggacggtc tgaatcacgc tggtaacgag tgcgcgaatt tcttcgtcgt tggtcgggcg
     1741 ctctgcacgg gcacgggctt cggtggcatc tgccaacatc agcaaggctg tttcgcgcga
     1801 agccgggcgt ggaccgggat aacggaattt ttcaatgtcc actttagaga tgtcgccacc
     1861 tacggcttcc atggcttggt tgtattgata gtgtgtgatg agcgtgccat gatgttcaag
     1921 gatgaaatca tgcaagcggc gcggaaggcg atgtttcttg gcaagctgca caccatcagt
     1981 aacatgccga atgatggtgg cagaaacttc ttcggggtta gcattattat gtgcgttgag
     2041 attgccctgc acttgattct caataaagaa gttcgggttg agcgccttgc caatatcgtg
     2101 gaataaagcg cctacgcgag tgagcaaagg gtctgcactg attttttcgg cggcttgctc
     2161 tgccaaatta gcaacctgca aactatgttg ataggtgccg ggcgcattgc gtaagaaaaa
     2221 ttgtaaaagc ggaaagtcgg ggcgtgaaat atctagtaat tgcaaggcag tggtcagccc
     2281 gaggatttgc gcaaagatgt attgcaataa aagcgcgatg ctcgcagaag agaagcctgc
     2341 aaaggcgatc atgcccatgt attgaagaat gccaagccaa tccaatgtga tgagcggaat
     2401 gcgaaatgcc atcagcaccg cggcacctga aatagaaatg gcaatgcctg ctcgcaagaa
     2461 gccccagaaa cggcgtgccg gacccaacgc cagcagtccg atcagcgatg tgaacatgta
     2521 atagggaata agatcggcag agttgggcaa accaaatgca gataagaccg ccaacggaat
     2581 ggcaaagacc aagcccactt ccatgctaaa taaggcagtg agcaataacc cagccgcttg
     2641 gattggatag ccatagggcg caagagtccg ttctgctaac agacgagccc ctgctaaaaa
     2701 gaccacaaag agcagggaga tgatcgtgag attcttcggg tcgttcagga atacagaacg
     2761 cggtttgcga taaaaataaa tgggaacaaa agcggcgcac agcaaaacga tagcagccgt
     2821 agcagcaatg tcctcccaac gctgaccggg acgaatcata cctagttgtt gaaaggcttc
     2881 cacatctgcc ggtgtaatga tctcccctgc tgggacgatg gtttcgcctg ctttataggt
     2941 ttgcacaatg ggttgaatgg cttcgcgcgc gcgcaacttc tcagcgtcgg tcaattcctg
     3001 gctgaagaag ctgtttgcga tcacaaacgg cgaaaccaat tctgtcacca gttcggtttg
     3061 ctgttcatta aaggtcaggc ttacagaaga agagatgctc gattgtgccg cttcaacacg
     3121 gtcttcgtaa atggcacggc gcatcacctg ctccagaacg cgcacggctt catcttgaac
     3181 agattgccaa cgtgtacttg aaatccccag caggtaatcg atcgtctcgt ctttcagacg
     3241 caagtcactc agtgataaca aactctgacg catttcttca ttggtcagtt ctttattatc
     3301 gcgaatggca gagatattca aaagagaagt gcgcaaacgt tcgatctgtg cgcgggtgat
     3361 ggacgggtct ggcgggctat ataccgattg aaccgcgctc tcagcggctt tgcgagcttc
     3421 ttccgtgcgg atctcactca catactcaat atcacggggc gaccgtatcg aagattgagt
     3481 cacttcccca atatcataag tctgctgatt ggggtttaag ctcaaaggcg aaatgagcat
     3541 accgaatgaa gccagtgcaa cgacggcgat catcaggtat tgcaatattc gagtacggcg
     3601 tggaacgggg gggcgtgtgg tcatacgaac g
//
LOCUS       DAOQJE010000276         3625 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_16126_length_3625_cov_5.407563, whole genome shotgun sequence.
ACCESSION   DAOQJE010000276 DAOQJE010000000
VERSION     DAOQJE010000276.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3625)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3625)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3625
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16126_length_3625_cov_5.407563"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..255
                     /locus_tag="PLT08_08385"
     CDS             <1..255
                     /locus_tag="PLT08_08385"
                     /inference="COORDINATES: protein motif:HMM:NF013584.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase family protein"
                     /protein_id="HNE04521.1"
                     /translation="PTCPTTAFKLGEKTSDPLEMYLSDIYVVSTNPAGVPAIALPAGF
                     SNNMPVGMQLIGKHLDEATLFQVAHAYQQVTDWHKQQPSL"
     gene            252..1121
                     /locus_tag="PLT08_08390"
     CDS             252..1121
                     /locus_tag="PLT08_08390"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HNE04522.1"
                     /translation="MSITFASILCGISGMFGWGIYDFLGGVFAKKIGSFKSLFWSQLA
                     GLVSLIVLAIFLKPNFHLPLLALALFPIASIVYSAGYLFFFKGFEVGNVSIVAATMNL
                     WAVFTMIFAFTFMGQRLSSTQTIGVLLILAGVLLASVNWSEIREQKFQLSAGVREAVA
                     GAFFFGIFWNISEIVSEEIGWLYTTLLVKLGIIVFLLLFSAIAKRELGLGKIASKTKS
                     AILLMGIIEAGAVAIVNYGLTIGDAILITPIASALSIVTILLAIIFLKDKITKIQGLG
                     IATAITGIVVTGF"
     gene            complement(1118..1612)
                     /locus_tag="PLT08_08395"
     CDS             complement(1118..1612)
                     /locus_tag="PLT08_08395"
                     /inference="COORDINATES: protein motif:HMM:NF017015.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HNE04523.1"
                     /translation="MNAEAFRHFFNYHFAENRKILEHVAALTFEQFTQKVDYSRGSIR
                     EQLIHLIDAEDVWISELRGVQPSEPLPETTEVDDRDAIRSLWDVVEQNTRTYLANLQD
                     NQLVSKPITDPEEDKELIVWQVLLHIVNHATDHRAQLLRALHDLGVDTKSQDYIFYVY
                     ENPL"
     gene            1673..2095
                     /locus_tag="PLT08_08400"
     CDS             1673..2095
                     /locus_tag="PLT08_08400"
                     /inference="COORDINATES: protein motif:HMM:NF022072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HNE04524.1"
                     /translation="MTKISTSLTINASQEAIWKVLSDVAHWQEWTPTVTKVEMLDTPE
                     IKLGNHYKVIQPKLQPVVWTVTELNSANFTWESKSPGMHMEAEHVVKSINANQSEVTL
                     TFAFNGWLGNLIGRMYGKMTEEYIRTEAQSLKKKAESA"
     gene            2109..2735
                     /locus_tag="PLT08_08405"
     CDS             2109..2735
                     /locus_tag="PLT08_08405"
                     /inference="COORDINATES: protein motif:HMM:NF016085.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF402 domain-containing protein"
                     /protein_id="HNE04525.1"
                     /translation="MGVTTFKSIGEYATLRFVESKVFGAFPTIVVQDSPEFIVLYMPT
                     GVLGKNVDHKPATNELFSADKIDIIDFQWKRTDVLMLIVPGEAFSTYVMWDAGTKNLD
                     CWYVNLQEPIRRTSIGFDTMDNMLDVVISPDMNTWQWKDDDEFEAAQSIGFYSAEKAR
                     AIRAEGEKAVQLITKERRTLYEKWKTWEANPEWELPILSPDWQRMDLG"
     gene            2737..3426
                     /locus_tag="PLT08_08410"
     CDS             2737..3426
                     /locus_tag="PLT08_08410"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: protein motif:HMM:NF024744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004721 - phosphoprotein phosphatase
                     activity [Evidence IEA];
                     GO_function: GO:0004725 - protein tyrosine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-protein phosphatase"
                     /protein_id="HNE04526.1"
                     /translation="MATNRVLNWQGCNNVRDLGGLTASNGSKTRWGAVVRGDQPAKLT
                     PEGWGELYEHGIRTIVSLRTYGFNEKDHPVVTPPYPDIHVMSVEIEDVTNKKFVEKWA
                     TSDLWGTPLYWTDALNLWANRHADALKAIAQAQPGGVFFHCIRGHDRTGIVSFLLLSL
                     LGVSLKDITADYELSADPVRDKLLAERNTTAHEVLQKTHAGINIENYLLNGGMTQDDL
                     AALRARLLTNK"
     gene            3546..>3625
                     /locus_tag="PLT08_08415"
     CDS             3546..>3625
                     /locus_tag="PLT08_08415"
                     /inference="COORDINATES: protein motif:HMM:NF014930.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE04527.1"
                     /translation="MTQYEPVIGLEIHGELLTNSKMFCSCS"
BASE COUNT          964 a          957 c          832 g          872 t
ORIGIN      
        1 ccaacctgtc ccaccaccgc cttcaaactc ggcgagaaaa ccagcgaccc gctcgaaatg
       61 tacctctcag atatttacgt cgtttcaacg aatcccgctg gcgtccctgc cattgcactc
      121 cccgctggct tctccaacaa catgcccgtc ggtatgcaac tcatcggcaa acacttggac
      181 gaagccaccc tcttccaagt cgcccacgcc taccagcaag tgactgattg gcacaagcaa
      241 caaccaagtt tatgagcatt accttcgcaa gcatcctgtg tggaatatca ggcatgttcg
      301 gctggggaat ctacgatttc ctcggcggcg tcttcgctaa aaaaataggc tcctttaagt
      361 ctctgttttg gtcgcagttg gctggcttgg tttccctaat tgtgcttgcg atattcctca
      421 aacccaactt tcatttaccc ttgcttgcgc tggctttatt tccgatcgct tccatagttt
      481 attcggcagg gtatttgttc tttttcaaag gctttgaagt tgggaatgtt tccatcgtcg
      541 cagcgaccat gaatctgtgg gcagtgttca cgatgatctt cgccttcaca ttcatgggac
      601 agcgtctttc ctccactcaa accatcggcg ttctgctaat cctcgctggc gtcctgctgg
      661 catccgtcaa ttggagcgaa attcgggagc agaaatttca actctcggca ggcgtcaggg
      721 aagcggtggc aggagcgttc ttcttcggca ttttttggaa catcagtgaa attgtttccg
      781 aagagatcgg atggctgtac acgaccctgc tcgtaaaatt gggcattatc gtttttctcc
      841 tgcttttctc agctattgcc aaacgagagt tggggctggg aaagattgca tcgaaaacaa
      901 aatccgccat cctgctgatg ggcatcatcg aagcgggcgc agtggcaatc gtcaactacg
      961 ggctgaccat cggcgatgcg atcctgatca cgcccattgc atctgccctt tccatcgtga
     1021 ccattttatt ggcgatcatt ttcttgaaag ataaaatcac gaaaatccag gggctcggaa
     1081 ttgcaactgc aattaccgga attgtcgtta caggcttcta gagtggattt tcgtagacgt
     1141 aaaaaatgta atcctgagat ttcgtgtcca cgcccagatc atgcaaggcg cgtagcagtt
     1201 gagcacgatg atcggtggca tgattaacaa tatgaagcaa gacctgccag acgatcaact
     1261 ccttgtcctc ttcgggatca gtgatcggct tggagaccaa ctggttatct tgtaagtttg
     1321 ccagataagt gcgagtgttc tgctcaacca catcccacaa cgagcggatg gcatcacgat
     1381 cgtcaacctc agttgtttct ggcaacggtt cagacggttg aacgccacgc aactcgctga
     1441 tccagacgtc ctcggcatca atgagatgga tgagttgttc ccgaatagag ccgcgagagt
     1501 aatccacttt ttgagtgaac tgctcaaaag tcaacgcagc gacatgctcc aatatcttgc
     1561 ggttctcggc aaagtgatag ttgaaaaaat gacgaaaggc ttctgcattc ataaagttgc
     1621 tccttttact aagtaaattt tattatagcg caaatgttct aggaatcaaa atatgacaaa
     1681 aatttcaacc tccctcacca tcaacgccag ccaggaagcc atctggaaag tcctctccga
     1741 tgtcgcccac tggcaagagt ggactccgac tgtgaccaaa gtcgaaatgt tggatacgcc
     1801 tgaaataaaa ctcggtaatc actacaaagt gattcaaccc aaactccaac ccgttgtttg
     1861 gacggtcaca gaacttaatt ctgccaactt cacatgggag tcaaagtccc cgggcatgca
     1921 catggaagcg gagcatgtgg tcaagtcgat caatgcaaat caatctgaag tcacactcac
     1981 cttcgccttc aacggctggc ttggcaatct cattggcaga atgtatggaa agatgaccga
     2041 ggaatacatc cgaaccgaag cgcagtcgct gaaaaagaaa gcagaatctg cttaatagga
     2101 gatttgactt gggcgttaca accttcaaat ccataggtga atacgcaact ttgcgctttg
     2161 ttgaaagcaa ggtctttggt gcttttccaa caatagttgt acaagactct cctgaattta
     2221 tcgttttata catgccaact ggcgtacttg gaaaaaatgt cgatcacaag cccgcaacca
     2281 acgaactatt ttccgctgac aaaatcgata ttatagactt tcagtggaaa agaaccgatg
     2341 tcttgatgtt aattgtgccc ggtgaagcat tcagcaccta cgttatgtgg gatgccggca
     2401 caaagaacct ggattgctgg tatgtcaact tacaggagcc tatccgccgt acaagcatcg
     2461 gctttgatac gatggataat atgctcgatg ttgtcatcag cccagatatg aacacctggc
     2521 aatggaaaga tgacgacgag ttcgaagcag cgcaaagcat cggcttttac tctgccgaaa
     2581 aggcccgcgc aattcgggct gaaggcgaga aggctgtaca actgatcacg aaggaaaggc
     2641 gcactttata cgaaaaatgg aagacctggg aagccaatcc tgaatgggaa ttgcccatcc
     2701 tttcccccga ctggcaaaga atggatctag gatagcatgg ccacaaaccg tgttctaaac
     2761 tggcaaggct gcaacaacgt ccgcgatctg ggcgggctga cggcttccaa cggaagcaaa
     2821 acccgttggg gcgctgtcgt gcgcggcgat cagccagcca aactcacccc agaaggttgg
     2881 ggtgagttat acgagcacgg catccgcacc atcgtgtcgt tgcgtacgta cggcttcaat
     2941 gaaaaggatc accccgtcgt tactccaccc taccccgata tccacgtgat gtcggtcgag
     3001 atcgaagatg tcaccaataa aaagttcgtc gaaaaatggg caacttccga tctttggggc
     3061 actcctcttt attggaccga tgcactgaat ctctgggcaa atcgtcacgc ggacgcgctc
     3121 aaagccattg cccaagccca acccggtgga gtcttcttcc attgcatccg cggacatgac
     3181 cgcaccggga tcgtttcctt tttattgctc tccctgcttg gcgtctcgct gaaagacatc
     3241 accgccgatt acgaactcag cgcagacccc gtacgagata aacttctggc agagcgcaat
     3301 acaaccgccc atgaagtcct gcaaaaaact cacgcaggca tcaacatcga aaactacctc
     3361 ctcaacggcg gcatgaccca agacgatctc gccgcccttc gcgcaagact actgaccaac
     3421 aaataatcat ccttcgtaag ggcgacccgt ccgggtctga ccaatacctt gccaagcaaa
     3481 atagacttgc aaaaacataa actatttggc attgacgggt cgcccttaca acaacgagag
     3541 aaaccatgac ccaatacgaa cccgtaatcg gacttgaaat tcacggcgag ttactcacca
     3601 actccaaaat gttctgcagt tgctc
//
LOCUS       DAOQJE010000277         3623 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_16140_length_3623_cov_6.798487, whole genome shotgun sequence.
ACCESSION   DAOQJE010000277 DAOQJE010000000
VERSION     DAOQJE010000277.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3623)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3623)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3623
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16140_length_3623_cov_6.798487"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            62..484
                     /locus_tag="PLT08_08420"
     CDS             62..484
                     /locus_tag="PLT08_08420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE04528.1"
                     /translation="MNHMYQQRALINGFTLKRFVTQTAIILLLGGIISASIVILDWDM
                     ELLFIACQIVGYTLFTTVSVIHSRLTQFWVMIVADGLVGLIPFIILVGYCYFHIENTA
                     CSVRSVKGNSIAFWVFPSIIMWILWILAPLGKTRRRYG"
     gene            784..1266
                     /locus_tag="PLT08_08425"
     CDS             784..1266
                     /locus_tag="PLT08_08425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE04529.1"
                     /translation="MSKDYYIVGTKYGRGIEDVYPDMLALGAVSVGFSRQIDLSDWYG
                     ASEKDIIEHLKKLGESEISYHNLKRFLNIKAGDLIAIKRSGSPLGNMARLVIAGYAIA
                     EARDGKVYCHDPNGLGHLIYVKFLNTESDKEFSLGYGQAVHKLTRESHIRQIFSDYGA
                     "
     gene            1511..2506
                     /locus_tag="PLT08_08430"
     CDS             1511..2506
                     /locus_tag="PLT08_08430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002670781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine-specific methyltransferase EcoRI family
                     protein"
                     /protein_id="HNE04530.1"
                     /translation="MPNKNELLHKAGKHKKDEFYTQLSDIERELKHYKNHLKGKVVYC
                     NCDDPRVSNFFHYFSYNFEKLGLKKLIATCYKNQNMDLFSQNNSEQAIYLEYVGDKNK
                     NSIPDPKEIGIKPLKGDGDFRSKECIELLKQADIVVTNPPFSLFREYVAQLIEYDKKF
                     IIIGHQNAISYREIFKLIMDNKLWLGYGFNRNMAHFINPNYEDYAADADHKEGMIRVS
                     GVVWFTNLDIQKRHEDLILYKTYRGNESEYPKYDYYDAINVDKTKDIPMDYKGQIGVP
                     ITFIDKYNPDQFEIIDGIGRYSMLTGPTKKTKGTYLTKVDGKPKYARIVIKNKRL"
     gene            2519..3601
                     /locus_tag="PLT08_08435"
     CDS             2519..3601
                     /locus_tag="PLT08_08435"
                     /inference="COORDINATES: protein motif:HMM:NF015210.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF262 domain-containing protein"
                     /protein_id="HNE04531.1"
                     /translation="MKIELKEITVRELTNGYQDNEENGVIGYGGKLDIRPPYQREFIY
                     KDKQRDAVIDTIIKKFPLNVMYWAVRADGNFEVIDGQQRTISVSQFVEGDFAFQNRYF
                     HNLQKDEQKQILDYKLMVYLCSGEDSEKLEWFKTINIAGEKLTDQELRNAVYAGSWVS
                     DAKRYFSKNNCPAYNMGNDYLSGAPIRQDYLETTIEWISNSSIEKYMAEHQHSPNANE
                     LWLYFQSVINWVKAVFPKYRNEMKGIQWGFLYNEFKDKNPDSKKLEKEITKLMLDEDV
                     TNKKGIYSYVLTRKEKFLNIRAFSDNQKREAYERQKGICVVCKQHFELSEMEADHRKP
                     WHEGGKTSAENCQMLCKEDNRRKSGK"
BASE COUNT         1252 a          639 c          742 g          990 t
ORIGIN      
        1 ccgccccagc gcaggtaacg catgccgttg ggcagcttaa ctgcaacata gaaaaacatt
       61 cttgaatcat atgtaccagc aacgtgctct aataaatggt ttcacgctaa aaagattcgt
      121 aactcaaaca gcaataatcc ttttgttggg tggaattatt tccgcctcaa tagttatctt
      181 agactgggat atggaattgt tgttcatcgc ctgccaaatt gtgggatata cattgttcac
      241 tacagtttca gtcatccatt ctcgccttac acagttttgg gtgatgatag tcgcagatgg
      301 tcttgttgga ttaataccgt ttattatttt ggtgggttat tgttactttc atattgagaa
      361 taccgcttgc agtgtgcgtt cagtaaaagg taatagtatt gcgttctggg ttttcccatc
      421 cataataatg tggattcttt ggattcttgc tcctcttgga aaaacgagaa gaaggtatgg
      481 ttaagtgcgc tggaaattgt attgggttgc actatactac tttgaaccaa tttcaatata
      541 atggagtttg aaggcaagag gctgcccaac accgcgtgca gccgacttgg ggtacgcacc
      601 gcgttatctt ggcatttttc tggcttggag tttttcccgt ttcgacggcg aatccacgct
      661 ccaaccccaa gcggctaacg ccagccgttg ggctgttcct tgcaaaacaa aataattact
      721 gaaaatcaaa aacatcgcca ttaatcaaga ttgagaatcg ggttgcaaag gagtgcaatc
      781 acaatgtcaa aagattacta tattgttggc acaaagtatg ggcgcggcat agaagatgtt
      841 tacccagata tgcttgctct tggcgcagtg agcgtcgggt tttctcgcca aattgattta
      901 agtgattggt atggcgcaag cgaaaaagat attattgaac atctaaaaaa attaggggag
      961 tcggaaattt cataccataa cttaaaacgg tttctaaata tcaaagcagg cgaccttatt
     1021 gcaattaagc gtagtggatc gccattaggc aatatggcgc gactggtaat tgcaggttat
     1081 gctattgcag aagcaagaga cggcaaagta tattgccatg accccaatgg tttaggacat
     1141 ttgatctatg ttaaattcct aaatacagaa agcgataaag aattcagttt gggatatggt
     1201 caagccgttc ataagttaac tcgtgaatct catattcggc aaatattctc tgattatggc
     1261 gcgtgaaatg atatgtcgcc aaacaataca agagtctcta aacataaaaa cagcccaaca
     1321 aagcgtgcac ccgacgctgg ggattctggc gcgattccaa gcatttttct acgcctcagc
     1381 attttcccag ttggacggcg ttccgccgcc cgccccagcg cgggtaacgc aaaccgttag
     1441 gtggctgtct tgcataacaa agtaaaatag tggttggaaa ttaaaaacat tgcaaaccga
     1501 gatttaggat atgccaaata aaaacgaact tttgcataaa gcaggtaaac ataaaaaaga
     1561 tgagttttac actcaattat ctgatattga gcgagaacta aagcattaca aaaaccatct
     1621 taagggtaag gtggtttatt gcaattgcga tgacccgcga gttagtaatt tcttccacta
     1681 tttctcgtat aattttgaaa aattgggttt gaaaaaatta attgcaacgt gttataaaaa
     1741 tcaaaatatg gacttgttca gccaaaacaa ctctgagcaa gccatttatt tagaatacgt
     1801 tggagataaa aacaaaaata gcatacctga ccccaaagaa atcgggataa aaccgttaaa
     1861 aggtgatgga gattttcgta gtaaagaatg tatcgaacta ttaaaacagg ctgacattgt
     1921 agtaactaat ccgccttttt ctttattcag agagtatgtc gcccaactaa ttgagtacga
     1981 taaaaaattc atcattatcg gacatcaaaa cgcaatttct tacagagaaa tattcaaact
     2041 cattatggat aataaattgt ggctcggata cggctttaat cgtaacatgg cacattttat
     2101 aaatcccaat tacgaagatt atgcagcgga cgcagaccat aaagaaggca tgattagagt
     2161 ttctggcgtg gtatggttta ctaaccttga tattcaaaaa aggcatgaag atttaattct
     2221 ctacaagaca tatcgtggaa atgaaagcga atacccaaaa tatgattatt atgacgctat
     2281 caatgtagat aaaacaaaag atatacctat ggattacaaa ggtcaaattg gtgttccaat
     2341 aacctttatt gacaaataca atcctgacca gtttgaaatc attgacggaa tcggcagata
     2401 cagcatgtta actggaccga ctaaaaaaac aaaaggaaca tatcttacta aagttgatgg
     2461 caaaccaaaa tatgctcgta tcgttataaa aaataaaagg ctctaaattg agtaaacaat
     2521 gaaaatcgaa ctcaaagaaa ttaccgtccg tgaattgacc aatggatacc aagacaacga
     2581 agaaaacggc gttattggtt atggtggcaa attagatata cgcccgcctt atcaaagaga
     2641 gtttatctac aaagataaac aaagagacgc agtaattgac acgattataa aaaaatttcc
     2701 gttgaatgta atgtattggg ctgttcgtgc agacggcaac tttgaagtta ttgacggaca
     2761 acaacggact atttcagtca gtcaatttgt agaaggtgat tttgcgtttc agaatagata
     2821 ttttcacaat ttgcaaaaag atgagcaaaa acaaatttta gattacaaac ttatggttta
     2881 tttgtgcagt ggcgaagata gcgaaaaact tgaatggttc aaaactatta atattgcagg
     2941 agaaaaactt accgaccaag aattgagaaa tgctgtctat gctggttcat gggtttctga
     3001 cgcaaaaagg tatttcagca aaaataattg tccagcctat aacatgggca acgattattt
     3061 aagcggtgcg ccaatcagac aagattattt agaaaccaca atcgaatgga ttagcaacag
     3121 ttccatagaa aaatatatgg ctgaacatca gcatagccca aacgctaatg aattatggct
     3181 ttattttcaa agcgttatca attgggtaaa agcggtgttt ccaaaatacc gtaatgagat
     3241 gaaaggtatc caatggggat tcctttacaa cgagttcaaa gataagaatc cagattctaa
     3301 aaaacttgaa aaagaaatta caaagttaat gctagacgaa gatgtaacaa ataaaaaagg
     3361 tatttattca tacgtcctca ctcgaaaaga aaagtttttg aatatccgtg catttagcga
     3421 taatcaaaaa cgtgaagcat acgaaaggca aaagggaatt tgcgtagttt gcaaacagca
     3481 ttttgagtta agcgaaatgg aagcagacca tagaaagccc tggcacgaag gcggtaaaac
     3541 atccgctgaa aattgccaaa tgttgtgcaa ggaagataac cgaaggaaat ctggaaaata
     3601 aagttgtgtt aaagtcaaac gcc
//
LOCUS       DAOQJE010000278         3618 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_16194_length_3618_cov_6.299747, whole genome shotgun sequence.
ACCESSION   DAOQJE010000278 DAOQJE010000000
VERSION     DAOQJE010000278.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3618)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3618)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3618
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16194_length_3618_cov_6.299747"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(124..1323)
                     /locus_tag="PLT08_08440"
     CDS             complement(124..1323)
                     /locus_tag="PLT08_08440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HNE04532.1"
                     /translation="MDQNETLSGTNNAQGDHNNQSMEALLASESLSIDLPKAGEIRKG
                     TIASISQNQILISIGAKSEGVVAGKELEQLTEEERAALKVGQELDVFVIIPEDNNGNV
                     VLSLKRAFEQIAWENVEKMIADETVFDTKIIGFNKGGLIAAIGSLRGFIPSSQISAAR
                     RAQSTGDKPEQRYQKMVGQPISVRIVEVDRERRRLILSERAANTESRSELKERVIGEL
                     EEGKSYTGRVTSLADFGAFVNINGADGLVHLSEISWDHVTHPKEVLEVGQEVNVKVIN
                     IDRDKKRIGLSIRALQDDPWKNRMSKFSVGQLVEGVITRLTKFGAFARLEGDIEGLIH
                     ISELSENRVEHPKEVLHEGDVKTLRIIRIDVDQHRIGLSLRKVDSAAFADKDFKMLTK
                     DFGGDEE"
     assembly_gap    1543..1642
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1643..2422
                     /locus_tag="PLT08_08445"
     CDS             <1643..2422
                     /locus_tag="PLT08_08445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNE04533.1"
                     /translation="VGTLDIEKAIQKGERHFEPGVLANANRGLLYIDEVNLLDDHVVD
                     ILLDSAAMGVNTVEREGISFAHPARFILVGTMNPEEGDLRPQLLDRFALSVEIVGIRE
                     ARERMTIMERNISYERDPEAFRKQWQPKEDELSQQIAKARELVDQVKYTSRDLLSIAA
                     LTASLNVDGHRADMVILKAARAEAAFEGRTKINDHDIALAAELTLPHRIKRTPFQQAE
                     MTTEQLQERIEQLAGQSMPNEEEDDSSESQEKGSDKEEKKT"
     gene            2476..3387
                     /locus_tag="PLT08_08450"
     CDS             2476..3387
                     /locus_tag="PLT08_08450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HNE04534.1"
                     /translation="MENREVFASSNANWWEGGQKVKAGDTFQPRKLNTPLDKLTRKQA
                     GRRSLTKTERKHGRYIMARPAGDNLTDIAFDATIRAAAPFQKQRTEERKKLAFSIKRS
                     DLQRKIRVKRVSNLVLFLVDASWSMAVAERMNATKGAILSLLTDAYQRRDRVGLIVFQ
                     KDRATLVLSPTNSVQLAQRALMDIPVGGKTPLSAGLFMAHDVISHEKHIHPDVEPLLI
                     VLTDGAGNVSLGTLPPQEEGFKFAEMIAMEKIRSVVINMEHAAFDQGLAQQLANHLEA
                     PCFSLNELKAETLYNAVVDEMSKPKKK"
     gene            complement(3422..>3618)
                     /locus_tag="PLT08_08455"
     CDS             complement(3422..>3618)
                     /locus_tag="PLT08_08455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE04535.1"
                     /translation="LNGERTERTELQMVKSKPFPFPPEPFRSPIINFTRWSLDQADRN
                     QGKRNLWLKTLDALGLGFDS"
BASE COUNT          889 a          950 c          865 g          814 t
ORIGIN      
        1 tacggtgcgc gtataatcgc ggtcgtactt gagcatgttg taggcaatat cggcgtgagc
       61 gtcaactaag agtggcatgg gcataattga aaagcgcacc ttgcggtgcg ctctttttgt
      121 tttttattct tcgtcgccgc cgaagtcttt cgtcagcatc ttgaagtctt tgtcggcgaa
      181 agcggcagag tccactttgc gcaggctcaa gccaatgcgg tgttggtcaa catcgatgcg
      241 gatgatgcgc aaggtcttga catcaccttc gtgaagaact tccttgggat gttcaacgcg
      301 gttctcactt aactcagaga tgtggatgag accttcgata tcgccttcga ggcgggcaaa
      361 agcaccgaac ttggtcaggc gggtaatgac accttccacc aactgaccca cgctgaactt
      421 ggacatgcgg ttcttccaag ggtcatcctg caaggcacga atggacaagc cgatgcgctt
      481 cttgtcgcgg tcaatgttga tgaccttgac gttaacttcc tgacccactt cgaggacttc
      541 cttggggtga gtaacatggt cccaagaaat ttcggagaga tgcacgagac cgtcggcacc
      601 gttgatgttg acgaaggcac cgaagtcggc gaggctggtc acacgaccgg tgtaagactt
      661 gccttcttcc aattcaccga tcacgcgttc cttgagttct gaacgagatt cggtattggc
      721 agcgcgctca gagagaatga ggcggcggcg ttcgcgatcc acttccacaa tgcggacgga
      781 gatcggctga ccgaccatct tctggtagcg ttgttcgggc ttatcacccg tagactgcgc
      841 acggcgagcg gcgctgattt gtgaagatgg aataaatcca cgcaggctgc caatggcagc
      901 gataagaccg cccttgttga aaccaatgat ctttgtatcg aacacggttt catcagcaat
      961 catcttttcg acgttttccc aggcaatctg ttcgaacgca cgtttgagag aaagcactac
     1021 attcccgttg ttatcttccg ggataataac gaacacgtca agttcctgtc cgactttcag
     1081 cgcagcgcgc tcttcttcgg tcagttgctc caattccttg ccagcaacga cgccctcgga
     1141 cttcgcccca atgctgatca ggatctggtt ttgtgaaata cttgcaattg tccctttgcg
     1201 gatttcgcct gctttaggaa gatcaatgct caacgactca gatgctaata gcgcctccat
     1261 cgattgattg ttgtgatctc cctgcgcatt atttgtcccg ctcagggttt cattttgatc
     1321 catcactcgt gactccagtt aaaagaaata tgaacaagat tatacaagtg aaacgacaag
     1381 ttgacaaccc taataagagg aaggaacaca taatttgcga gtaagtttcc aaaaaaaggg
     1441 cgttataatc acgggctgga gtaaacaaat gcccgctatt ttccccttta cagctatcgt
     1501 tggacaagac cgcatgaagc gtgcgctcat cctcaacgcc gtnnnnnnnn nnnnnnnnnn
     1561 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1621 nnnnnnnnnn nnnnnnnnnn nngttggaac gctcgacatt gaaaaagcca ttcaaaaagg
     1681 cgaacgccac tttgaacccg gtgtgcttgc caatgccaac cgcggcttgc tctacatcga
     1741 cgaagtgaac ttgctggacg atcatgtggt ggatattctt ttggactctg ccgccatggg
     1801 cgtgaacacg gtggagcgtg aaggcatttc ctttgctcac cccgctcgct tcattctcgt
     1861 tgggacgatg aatcctgaag aaggcgatct tcgccctcag ttgctggatc gcttcgccct
     1921 ctcggtggag atcgtaggaa tccgcgaagc ccgcgaacgc atgaccatca tggaacgcaa
     1981 tatttcctac gaacgcgatc ccgaagcctt ccgcaagcaa tggcaaccca aagaagatga
     2041 actctctcag caaattgcca aagcccgcga actcgtagac caggtcaaat acaccagccg
     2101 cgatttgctc tccatcgctg cactcaccgc ttccctcaat gtggatggtc accgcgccga
     2161 tatggttatc ctcaaagccg cacgtgctga ggcggcattt gaaggtcgca ccaagatcaa
     2221 tgaccacgat atcgctctcg ccgcagaatt gactctgccg catcgcatca agcgcacacc
     2281 tttccagcaa gccgaaatga ccaccgaaca actgcaagaa cgcattgagc agttggcagg
     2341 tcagtccatg ccgaatgaag aggaagatga ctcttctgaa agccaggaaa aaggcagcga
     2401 caaagaggaa aaaaaaactt aaaactggaa gatgagcaat ccgaacaggg tccgcaagaa
     2461 ggcaaccccg aaccgatgga aaatcgggaa gtgttcgcaa gcagtaatgc gaactggtgg
     2521 gaaggcggac aaaaagtcaa agctggcgat accttccagc cgcgcaagct caatactcct
     2581 ttagataaac ttactcgcaa gcaggcgggt cgccgttcgc tcaccaagac cgaacgcaaa
     2641 catggtcgct acatcatggc ccgccctgct ggcgataact taacggatat cgcgttcgat
     2701 gcgaccatcc gcgctgcagc cccctttcaa aaacaacgaa ctgaagaacg caaaaagctc
     2761 gctttctcga tcaagcgcag cgacttgcaa cgcaagatcc gcgtgaagcg ggtttcaaat
     2821 cttgtcttat tcctcgtaga tgcttcctgg tctatggcgg tggctgaacg tatgaacgcg
     2881 accaaaggcg ccatcctttc gcttctcacc gatgcctatc agcgccgtga tcgcgtgggt
     2941 ctcatcgtct tccagaaaga ccgcgccaca ctggttctct cccccaccaa ctccgttcaa
     3001 ctcgctcaac gcgccctgat ggatatccct gtcggcggca agactccact ttcagcaggc
     3061 ttattcatgg cgcatgatgt tatcagccat gagaagcata tccacccgga tgtggagccg
     3121 ttgctgattg tgttgaccga tggagctggt aatgtctctc tcggcacctt accgccacaa
     3181 gaagaaggct tcaaattcgc tgaaatgatc gccatggaaa aaatccgctc ggtggtgatc
     3241 aatatggaac acgccgcctt cgatcaggga cttgcccaac aattggcaaa ccatctcgaa
     3301 gcgccctgtt tctcgctcaa tgagctaaaa gccgaaacgc tctacaacgc ggtggtcgac
     3361 gaaatgtcca aaccgaaaaa gaagtaaaaa agaggcagtc actagtgact gcctcttttt
     3421 attacgagtc aaaccccaac cccaatgcat ccaaggtttt caaccaaaga tttctcttgc
     3481 cttggtttcg atctgcctga tccaatgacc aacgcgtaaa gttgataatc ggagagcgga
     3541 acggttctgg cgggaatggg aagggtttgc tctttaccat ttgcaattcg gttcgttcag
     3601 tgcgttcgcc attcaaca
//
LOCUS       DAOQJE010000279         3618 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_16196_length_3618_cov_5.005052, whole genome shotgun sequence.
ACCESSION   DAOQJE010000279 DAOQJE010000000
VERSION     DAOQJE010000279.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3618)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3618)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3618
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16196_length_3618_cov_5.005052"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(13..1047)
                     /locus_tag="PLT08_08460"
     CDS             complement(13..1047)
                     /locus_tag="PLT08_08460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE04536.1"
                     /translation="MTLGRIAFLGSGETSLAGGRIFETLARLIPDPLRIAILETPAGF
                     ELNASLVAGRVADFLKTRLGNYKPVIDLIPARKKGTEFSPDNPEILKPLLQANMIFMG
                     PGSPSYVARQLKDTLAWDIIRARHRQGATLVFASAATISVGRWVLPVYEIYKVGEDVH
                     VKDGLDFFADFGMQVSFVPHWNNAEGGVDLDTSRCFIGMERFNQWRNLTPSDNIIVGL
                     DEHSGIIMDCEKGVCDVHGVSSVSVLKSDTSEMYASGSSFSLMELGNCAPPNPIEKGI
                     RPEVWEMITSAQSAETDGPSEEVLALLEQRQQARANKDFAESDRLRDQISSLGWTVQD
                     SKAGQTLVKK"
     gene            complement(1044..1508)
                     /locus_tag="PLT08_08465"
                     /pseudo
     CDS             complement(1044..1508)
                     /locus_tag="PLT08_08465"
                     /inference="COORDINATES: protein motif:HMM:NF012787.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin family protein"
     gene            complement(1529..2497)
                     /locus_tag="PLT08_08470"
     CDS             complement(1529..2497)
                     /locus_tag="PLT08_08470"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HNE04537.1"
                     /translation="MKRWKKILLFAFAFACVTTTLGPFAVPVPKLDGLVSEQEFVEPD
                     SKFIEINGVEIHYKEKGSGERTFILLHPFGGSTYSWRNVMDEFAKYGKVIAYDRPAFG
                     LTERPMPGDWETNPYGMKANVEILREMLDAFGIEKAVLVGNSAGGGVAVAFALEYPER
                     IDELILVAPGVGGGYGPQFPAWALPIMWTPQMRHLGPLMMRDYQESLPRTVEREWFDQ
                     SKLTDEIRAKHLQILKIKNWDKAFYELTFAPAYPELRPLLPNLKVPALIVAGQEDRLI
                     RSWYFEAISTEIPEAQLKTIPQCGHVPQEECPTEFMEVVLKYLHYQ"
     gene            complement(2516..3262)
                     /locus_tag="PLT08_08475"
     CDS             complement(2516..3262)
                     /locus_tag="PLT08_08475"
                     /inference="COORDINATES: protein motif:HMM:NF024107.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HNE04538.1"
                     /translation="MKKINWKIVAAVITAVLLVLVAGFVFWASDASAATDVALQALNS
                     DSKVNVSAENGWVIFTPAENSRPETGFIFYPGGKVDYRAYAPILKMISAKGYFVAVVP
                     VPLNLAFFDINAASRVEAAYPEVKNWFVGGHSLGGVAASSYAASHEEIRGVVFWASYP
                     ADDSLKTKNIPAVSIYGTNDGLATGDKLDASKALLLEDTKFVPIDGGNHAQFGSYGPQ
                     AGDNEATISPEDQWLQATDATVAFFAEVLK"
     gene            complement(3268..>3618)
                     /locus_tag="PLT08_08480"
     CDS             complement(3268..>3618)
                     /locus_tag="PLT08_08480"
                     /inference="COORDINATES: protein motif:HMM:NF015146.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase family protein"
                     /protein_id="HNE04539.1"
                     /translation="LKDMYITASGRCAPPDNKPSPEELNNCQPFLERELEIINPKVIV
                     CLGRIAFERILKIYSEKSSVYKFAHGASYQLANGKWLLCSYHPSQQNTSTKKLTPTMF
                     DAIWVKAKQLADRN"
BASE COUNT          974 a          891 c          831 g          922 t
ORIGIN      
        1 ggcggttctt ttttatttct tgaccaatgt ttgccctgct tttgagtctt gtacagtcca
       61 cccaagagaa gagatttgat cacgcaaacg gtctgactcg gcaaaatctt tatttgcgcg
      121 agcctgctga cgttgttcca gcaatgccaa tacctcttca gagggtccat ctgtttcagc
      181 agactgagca gaggtaatca tctcccatac ttccgggcga atacccttct caataggatt
      241 cggcggtgca caattaccta attccattaa agaaaaactg gagccagagg cgtacatttc
      301 agaggtgtca cttttcaaaa cagagaccga gctgacgcca tgtacatcac aaacgccttt
      361 ttcgcagtcc atgataatgc cggaatgttc gtctaaaccc acaatgatgt tatcggaagg
      421 cgtcaaatta cgccattggt tgaagcgttc cataccaata aagcaacggc tagtatcaag
      481 gtccacgcca ccttcggcat tgttccaatg tggcacgaag gaaacctgca tgccaaaatc
      541 tgcaaagaaa tcgagcccgt ctttcacatg gacatcttca cctactttgt agatttcata
      601 aacaggcaaa acccaacgtc ccacagaaat ggtagcagcg gaggcaaaga ccaaggttgc
      661 gccttgacga tgcctggcac ggatgatatc ccacgctagc gtgtctttta attgacgcgc
      721 cacgtagctg gggctgcccg gtcccatgaa gatcatatta gcttgtaata agggttttag
      781 aatttcgggg ttatcagggc tgaactctgt cccttttttg cgagcaggga tcaggtcaat
      841 cacaggtttg tagttaccca aacgcgtctt gagaaagtct gcgacgcgcc ctgcgacgag
      901 agaggcgtta agctcaaagc cagctggcgt ttcgagaatg gcgatccgca acgggtccgg
      961 gatgaggcgg gcgagggttt cgaagatgcg tcctccggca agagaagtct cgccggaacc
     1021 gagaaaggca atgcgtccaa gtgtcatact ttactggatg ctttattcaa ctcatctatt
     1081 acatcaaaga aaattttaat atctgggtaa tctttgcgat cggcactgta atgaatataa
     1141 cggatatagc catcgaggtc tacaatgcaa ttcatgggca tacgaccgaa tttaaatagg
     1201 tttacttctt gcttgtacaa tttggcaaca ctattttctg ggtctggcac gccaatgaag
     1261 gtaaggtctt cacgctgcca ataacgctgg aattgttcaa gcccatcagg acctacggca
     1321 atcaattcgg catttcgttc agtaaaaccc tcatacgaat cgcggaagcg cgccaactgc
     1381 gcgcggcaat gtggtcagat aaaaccacgc aaaaaggaaa ggatgaccgg tttctttcct
     1441 cgataatcag aaaggcggat gaggtttccg tttatatctt ctagtgaaaa atctggtgca
     1501 agttgaccgt tttgcagcaa tagagtactc attggtagtg taaatatttt agtacaactt
     1561 ccataaattc ggttggacat tcctcttgtg gcacatgccc gcattgagga atggtcttga
     1621 gttgcgcttc gggaatttca gtggagatgg cttcaaaata ccacgagcga atgagacgat
     1681 cttcctgccc agctacaatg agggcaggga cttttaagtt cggcagcaat gggcgtagtt
     1741 cagggtacgc tggcgcaaag gtgagttcgt aaaaggcttt gtcccaattt ttgatcttga
     1801 gaatttgcaa atgtttcgca cgaatctcat cggtgagttt ggattggtcg aaccactcac
     1861 gttccactgt gcgcggcagg ctttcttgat aatcgcgcat catgagcgga ccgagatgcc
     1921 gcatttgcgg cgtccacata atcggcaacg cccacgccgg gaactgcgga ccatacccac
     1981 cacccacacc gggcgcgaca aggataagct catcaatacg ttctgggtat tccaacgcaa
     2041 aggctactgc cacgccaccg cccgccgagt tgcccacaag gacagctttt tcaatgccaa
     2101 aggcatcgag catttcacgc aaaatttcga cattggcttt catgccatat gggtttgttt
     2161 cccagtcacc gggcatggga cgttctgtta gcccaaaggc tgggcggtcg tacgcgatga
     2221 ctttgccata ttttgcgaac tcatccatta catttcgcca agaatacgtg ctgcccccaa
     2281 aaggatgcaa caggatgaag gtcctttcgc cagaaccttt ttccttgtaa tgaatttcca
     2341 cgccgttgat ctcgatgaat ttactatcag gttcaacaaa ttcttgttcg cttaccaagc
     2401 catccaattt tggaacaggg acggcaaaag gccccagcgt ggtggtaacg caggcaaaag
     2461 caaaagcgaa taacagaatt tttttccagc gcttcatggt tagacgatag gcgatttatt
     2521 tcagaacctc agcaaagaaa gcaacggttg catcggtggc ttgaagccac tggtcttcgg
     2581 gagaaatagt cgcttcgtta tcgccggctt gaggtccgta agagccgaat tgagcgtggt
     2641 ttccaccatc aatcggaaca aatttagtat cttccaacag cagggctttg gatgcatcta
     2701 acttatcgcc tgttgcaagt ccgtcatttg tgccatagat cgaaaccgca gggatgttct
     2761 tggtcttcaa agaatcatca gccggatacg aagcccaaaa caccacgcct cgaatttctt
     2821 cgtgacttgc cgcataagaa gatgccgcca cgccgcctaa cgagtgccca cctacaaacc
     2881 aattcttgac ttcaggatac gccgcttcta cccgagaagc tgcattgata tcaaagaaag
     2941 ccaaattcaa tggaaccgga actaccgcta cgaaataccc cttggcagaa atcattttca
     3001 agatcggcgc atatgcgcga taatccactt taccaccagg gtaaaaaata aagcccgttt
     3061 caggtcgtga attttctgcc ggggtaaaaa taacccagcc attctcagcc gaaacattca
     3121 ctttcgaatc tgagttcaat gcttgcaaag ccacatcagt ggcggctgaa gcatcacttg
     3181 cccaaaacac aaaaccggca acaagtacca acaaaacggc ggtgatcacc gccgctacga
     3241 tcttccaatt tatttttttc atgcacatca attcctatct gcgagttgtt ttgccttcac
     3301 ccaaatcgca tcgaacatcg ttggggtcag tttttttgta gaggtatttt gctggctggg
     3361 gtggtatgaa caaagcagcc acttcccatt tgccagttga taagacgcgc cgtgagcgaa
     3421 tttatatacc gaactttttt cggaatatat ttttagaatc ctctcaaaag ctatccgacc
     3481 caggcaaaca atgaccttcg gattaataat ttctaattca cgttcaagaa aaggctgaca
     3541 attatttagc tcttctggac tcggcttatt atctggcgga gcgcaacgac ccgacgcggt
     3601 gatatacata tcctttag
//
LOCUS       DAOQJE010000280         3616 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_16215_length_3616_cov_5.786858, whole genome shotgun sequence.
ACCESSION   DAOQJE010000280 DAOQJE010000000
VERSION     DAOQJE010000280.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3616)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3616)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3616
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16215_length_3616_cov_5.786858"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..822
                     /locus_tag="PLT08_08485"
     CDS             <1..822
                     /locus_tag="PLT08_08485"
                     /inference="COORDINATES: protein motif:HMM:NF012546.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HNE04540.1"
                     /translation="EAYNLLHAPMADRELPDELPSDYNMQYEDVTVTNPDGMKLVGWF
                     IPSENGAVIIMQHGYKSTRSEMLNEAEMMHRHGYGILLTTVRAHDESEGELITLGYYE
                     MADLEAWYQYLISRDDIDPEKIGILGNSYGGMLSIQYAAQNQNIKAVVADCAFSSMKD
                     TVSTSVRHFTGLPDFPFVPLIVFWAENMTGISIDDIDATKWIPNISPRPVFLMQGGKD
                     TVISPTSGQILYDAAGEPKELWFDPELGHVDFDSERAEEFEARVSAFFDQYLLGQ"
     gene            829..1440
                     /locus_tag="PLT08_08490"
     CDS             829..1440
                     /locus_tag="PLT08_08490"
                     /inference="COORDINATES: protein motif:HMM:NF013933.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysE family transporter"
                     /protein_id="HNE04541.1"
                     /translation="MWVYLIQGVGYGFAAASQIGPFQTFLISQALTRGWKRALPGAFA
                     PFLSDGPIILLSVLVLSQLPDWAQRAMYIVGGLFVLYLAYGSYKSWRNFDETLAHPEL
                     SGQQTIFKAAMTNALSPGAYLFWMFVTGPILVQGWRETPINGLGLLIGFYTTFIICLI
                     AMIIVFGAASRLGPRLNRVLLGLSCIILFGFGLYQFWLGIFVN"
     gene            1451..2593
                     /locus_tag="PLT08_08495"
     CDS             1451..2593
                     /locus_tag="PLT08_08495"
                     /inference="COORDINATES: protein motif:HMM:NF024826.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding domain-containing protein"
                     /protein_id="HNE04542.1"
                     /translation="MQTDLLIIGAGPFGLSLAAYAKSLGMDFLVAGKPMEFWKKNMPA
                     GMFLRSASDWSLDPTDRFSIMNYLETLGKTPKDVEPISLEFYLQYAQWFQSGAGIETI
                     SEYVTRLDQKEDGFLATFEDGKVIEAKNVAIAIGMGYFKNYPAALTDILPVGRYEHTC
                     DAVQLSEYKGRRVLILGGRQSAFEWAALLNDGGAAEVHVVHRHESPKFAEADWSWVPP
                     LVDGMVADPAWFRGLPQAEKDAIVKRMWGEGRLKVEPWLEKRVMKPNTSIHQKASLLA
                     CVERSDGALDVKLDNGDAFVVDDVILATGYKVELERLSFLKNGNMFDKVEVKNGFPVL
                     DPHFQTTAKGLYFTSMPAGQDFGPFFGFTVSVRASAKIIGNALVSK"
     gene            2603..2908
                     /locus_tag="PLT08_08500"
     CDS             2603..2908
                     /locus_tag="PLT08_08500"
                     /inference="COORDINATES: protein motif:HMM:NF015925.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HNE04543.1"
                     /translation="MNIVLVHVHVKPEFVDAFKQASLENASNSVKEEGIARFDVIQQA
                     DDPTRFILVEVYKTVEAQSAHKETAHYLTWRDTVAEMMAEPRQGVKYSNIFPADGGW"
     gene            2911..>3616
                     /locus_tag="PLT08_08505"
     CDS             2911..>3616
                     /locus_tag="PLT08_08505"
                     /EC_number="1.1.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF012678.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HNE04544.1"
                     /translation="MSFEFATANRIIFGAGKLSGLGDQLKGSVKRLLLVRGRSADAIP
                     RVREILSAQGIQFNEFEVHGEPTVDVVREGVKFADGCDAVIGLGGGSVLDAGKAIAAL
                     ATNPNDVVDYLEVVGKGQPLIEKPLTYIAIPTTAGTGTEVTRNAVLDVPEHGVKVSLR
                     SPMMLPSLAIVDPELTYSLPPEITAASGLDALTQLIEPFVSVKANPLTDAICREGMKH
                     AAKSLRRVYQNGADKEA"
BASE COUNT          853 a          789 c         1001 g          973 t
ORIGIN      
        1 gaagcatata acttattgca tgcccccatg gcagatcgtg aactgcctga tgaattgcct
       61 tctgattaca acatgcaata tgaagacgtc actgttacca acccagatgg tatgaagcta
      121 gtgggttggt ttatcccatc tgaaaatggg gcggttatca tcatgcagca cgggtataaa
      181 tccacgcgca gtgagatgct caacgaagct gaaatgatgc accggcatgg ctacggcatt
      241 ttgcttacca cggtccgtgc tcatgatgag agtgaaggag aactcatcac acttggatat
      301 tatgaaatgg cagacctcga agcgtggtat caatatctta tttcgcgaga tgacatcgat
      361 cccgaaaaga tcggtattct cggcaattcg tatggcggca tgctttccat tcaatatgct
      421 gcgcagaatc aaaacatcaa agccgttgtg gcagattgtg ctttctcttc tatgaaagat
      481 acagtttcca ccagtgtgcg tcattttacg ggcttgccag attttccatt tgtgccactt
      541 attgtctttt gggcagagaa catgaccggt atcagcatcg acgatattga tgcaacaaag
      601 tggattccca atatcagtcc gcgcccggtg ttcttaatgc aaggcggcaa agacacggtc
      661 atttcgccca caagcggaca aattctttat gatgccgccg gagagccaaa agaactttgg
      721 ttcgaccccg aactcgggca cgtggatttt gacagcgaac gcgctgaaga attcgaagcg
      781 cgtgtatccg ctttctttga ccaatactta cttgggcaat aaatctatat gtgggtgtat
      841 ctcattcagg gtgtcggata cggcttcgcc gccgcctctc agatcggacc ctttcaaacg
      901 tttctgattt ctcaagcctt aacccgcggc tggaagcgtg ccctgcccgg cgcatttgcg
      961 ccattcctca gtgatggtcc tatcattttg ttgagtgtgc tggtattaag ccaactaccc
     1021 gattgggcgc agcgtgccat gtatattgtg ggcgggctat tcgttctcta tcttgcttat
     1081 ggttcatata aatcctggcg taattttgac gagacacttg cgcatccgga attatcgggt
     1141 cagcagacca ttttcaaagc ggcgatgacg aatgccctca gccccggagc ttatcttttt
     1201 tggatgttcg tgactggtcc tattttggtg caaggctggc gcgaaacgcc tatcaacggc
     1261 ttgggtctct tgatcgggtt ttatacgacc tttattattt gcctgatcgc aatgatcatt
     1321 gtcttcggag ccgcatctcg tttgggaccc aggttgaatc gtgttttact tgggctttcc
     1381 tgcatcatct tgttcggctt tgggctatat cagttttggc ttggaatttt cgttaactaa
     1441 ggagagtgga atgcaaacag atttacttat cattggtgcg ggcccttttg gtttgtcttt
     1501 ggcggcatat gccaaatctt taggcatgga ctttctcgtt gcaggcaaac ctatggaatt
     1561 ttggaagaag aacatgcccg cgggcatgtt cctacgctct gcgtcagact ggtcgctgga
     1621 cccaaccgat cgtttttcca ttatgaatta tctggaaaca ttaggtaaga ctccgaaaga
     1681 tgttgagccg atctctcttg agttctattt acagtacgct cagtggttcc aatctggagc
     1741 aggaatcgag accatctctg aatatgtcac acgcttagac caaaaggaag atggtttcct
     1801 tgcgaccttt gaagatggaa aggtcattga agccaaaaac gttgccattg ccattggcat
     1861 gggctatttc aagaattatc ccgccgcgtt aacagacatt ttgcctgttg gtagatatga
     1921 acacacctgt gatgccgtgc aactttctga atataaaggc aggcgggtgt taatcttggg
     1981 tggcaggcaa agcgcttttg aatgggcggc gttgttgaat gatggcggcg cggcggaggt
     2041 gcatgtggtg catcgccacg agtcaccgaa atttgccgaa gcggattgga gttgggtgcc
     2101 gcctctggtg gatggtatgg tggcggatcc cgcctggttc cgcgggctgc cacaggcgga
     2161 aaaagatgcc atcgttaaac gtatgtgggg tgaggggcgt ttgaaggtgg agccatggtt
     2221 agagaagcgc gtgatgaaac cgaacacgag catccatcag aaagccagtt tgctcgcttg
     2281 tgtggagcgc agtgatggcg cattggatgt gaaactggat aacggtgatg cattcgtggt
     2341 ggatgatgtt attcttgcca caggctataa ggtcgagttg gaacgattgt ctttcttgaa
     2401 gaacggcaat atgttcgata aggtcgaagt gaagaatggc ttcccggttc tcgacccgca
     2461 tttccaaacc acagccaaag ggttgtactt caccagcatg ccagccgggc aggattttgg
     2521 tccgttcttt ggtttcactg tttcggtgcg ggcttctgca aagatcatcg gaaacgcctt
     2581 ggtatctaaa taggagcttc aaatgaacat agttcttgtg catgtgcatg ttaaaccaga
     2641 atttgtggat gccttcaagc aggcaagcct tgaaaatgcg tcgaatagtg tgaaggaaga
     2701 aggtattgca cggtttgacg tcattcaaca agcggatgac ccgacgcgtt ttatccttgt
     2761 ggaagtgtat aagaccgttg aagctcaatc tgcccacaaa gagacggctc attacttgac
     2821 gtggcgtgat actgtcgcgg agatgatggc agagccacga cagggcgtga aatacagcaa
     2881 tatctttcca gcagatggcg gttggtaaaa atgtcctttg aattcgcaac agcaaatcga
     2941 atcatcttcg gggcagggaa gctttctggg ttaggcgacc agctcaaagg gagtgtgaag
     3001 cgtttgctgt tggtgagggg caggtcagcg gatgcgattc cgcgtgtgag ggagattctg
     3061 tctgcacagg gaatccaatt taatgaattt gaagttcatg gcgaaccgac ggtggatgtg
     3121 gtgcgcgaag gggttaagtt tgctgatggg tgcgatgccg tgatcggttt gggcggcggc
     3181 agtgtgctgg atgcaggcaa ggcaatcgcg gcattggcga caaaccctaa cgatgtcgtt
     3241 gattatttgg aagtggtggg gaaggggcag ccgctcattg agaaaccgtt gacttatatt
     3301 gccattccca ccacagcggg aaccgggacc gaagtgacgc gtaatgcggt gctggatgtg
     3361 ccggaacatg gcgtgaaggt cagcttgcgt agcccaatga tgttgccaag cctcgccatc
     3421 gttgaccccg agttgactta ttctttgccg ccagaaatta cagccgcttc tggtttggat
     3481 gcgctgacac agttgatcga gccttttgtt tcggtgaaag cgaatccact tacggatgcg
     3541 atttgccgtg aaggtatgaa gcatgcggca aaatctttac gccgtgtgta tcagaatggt
     3601 gcggataaag aagcac
//
LOCUS       DAOQJE010000281         3611 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_16251_length_3611_cov_5.376828, whole genome shotgun sequence.
ACCESSION   DAOQJE010000281 DAOQJE010000000
VERSION     DAOQJE010000281.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3611)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3611)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3611
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16251_length_3611_cov_5.376828"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..302)
                     /locus_tag="PLT08_08510"
     CDS             complement(<1..302)
                     /locus_tag="PLT08_08510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE04545.1"
                     /translation="MQTDTGIDTQLKEAVQLTGADWAVLAERVGGVWLVRASYHLTKA
                     AQNELTGIMAAASTDAWLCGALSGGHSRSAEIPENRSIGEGRFLAFPILSTSKLILV"
     gene            361..1701
                     /gene="trmFO"
                     /locus_tag="PLT08_08515"
     CDS             361..1701
                     /gene="trmFO"
                     /locus_tag="PLT08_08515"
                     /inference="COORDINATES: protein motif:HMM:NF003739.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate--tRNA-(uracil(54)-
                     C(5))-methyltransferase (FADH(2)-oxidizing) TrmFO"
                     /protein_id="HNE04546.1"
                     /translation="MNEVTVIGGGLAGSEAAWQLAQQNIKVKLYEMRPVNPTGAHVTS
                     DLAELVCSNSLGSNQADRASGVLKNELRRLNSLLVECAEDTALPAGAALAVDREAFAK
                     RVTERVEAHPNIELVREEVKEIPSSPVIVASGPLTSDSLSKSIAALSGDEHLFFFDAI
                     SPIVRAESINMDIAFRGSRYDKSEQEEGDYINCPFTKEEYYEFLTALKTAERIELRAF
                     EDAIKTGVKAGQFFEGCLPVEIIAERGDESLAYGPMRPVGLRDPRTGKRPYAVAQLRQ
                     DNLAGSLYNIVGFQTNLKFPEQRRVLRMIPGLANAEFERYGQMHRNTFIASPKLLRPT
                     LQHITREDLFFAGQLTGVEGYMGNIATGLLAGINMARLIQNKALLTLPKESMLGALCH
                     YITHADLKDFQPMKANFGILPDLNLGKKISKREKGQAYADRASQSLETYLQESS"
     gene            1701..2609
                     /locus_tag="PLT08_08520"
     CDS             1701..2609
                     /locus_tag="PLT08_08520"
                     /inference="COORDINATES: protein motif:HMM:NF016289.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family peptidase"
                     /protein_id="HNE04547.1"
                     /translation="MNKRAVVLYLSIILGLVLIVTGWYAASFLTYTSESVPEGFSGDR
                     AYADVQTQVDMGPRTPGSAGHAQIREWMRTELESAGWIVEVHESERLGHPIYNIIAKR
                     GKEPPQIILAAHYDTRFFADNDPDPAKHTEPVPGANDGASGVAVLLELARTLPADTTP
                     TWLVFFDTEDNGRVEGWDWILGSRAFVEEVPIQPQAVVIIDMIGDADLNIFYEKNSDP
                     TIRAEIWSTAAELGYGKQFIQEEKYNMLDDHTPFVEKGIPAVDMIDFDYPYWHTVQDT
                     PDKVSAESLKAVGDTLWHWVVKHGEK"
     gene            2739..>3611
                     /locus_tag="PLT08_08525"
     CDS             2739..>3611
                     /locus_tag="PLT08_08525"
                     /inference="COORDINATES: protein motif:HMM:NF012722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HNE04548.1"
                     /translation="MAVQASLQKLRPIWVERVSRELAAGEGVRSGFEEQLQRFYTLLE
                     QTIVTGDTAWLDPILFDWGHSPTQTNLEQADYHVSFVLDRMISLTIDIAGDHLEDKDA
                     LELLAAVIPILAHSLGIVVRYEMETRVAHISKELGSVQEKLQNLDQNKSKFISVAAHE
                     LKTPLTLIEGYASMMVDLVQNSEQAVMKNYLSGVNTGIQRLRDIIDDMIDVSLIDNQL
                     LTLNIQPMWTTHLLNLVRNEFKKPIAERNQTLNVYDFDGSDLMIYGDSERLYQALQNV
                     VSNAIKYTPDKGIIT"
BASE COUNT          970 a          937 c          872 g          832 t
ORIGIN      
        1 acgagaatca acttggatgt gctaagaatg ggaaaagcaa gaaagcgccc ctcaccgatc
       61 gaccggtttt ctgggatttc cgccgaacga gaatgccctc cgctgagcgc gccacacaac
      121 catgcatcag tagaggctgc cgccataata ccggtcagtt cgttttgagc agctttggtg
      181 agatgatacg aagcccgcac cagccataca ccgccaacac gttctgccaa caccgcccag
      241 tctgcaccgg tgagttgaac cgcttctttg agttgagtat cgatgcctgt gtctgtttgc
      301 atggtaaatg ctattcaaat tatacattat gcccatcgtc cactggtaaa atagcgccct
      361 atgaatgaag tgactgttat cggtggcggg cttgccggaa gtgaagcagc atggcagctt
      421 gcccagcaaa atatcaaagt aaaactctat gagatgcgcc cggtcaaccc gaccggtgcc
      481 catgtgacca gtgacctagc ggaactggtc tgttcgaatt cactcggctc caaccaagcc
      541 gaccgcgcct cgggcgttct gaaaaatgag ctacgccgac tcaattccct gctggtggag
      601 tgtgctgaag atacagcctt gcccgccggc gctgctttag cggtagaccg tgaagccttt
      661 gcaaaacgcg tcacagaacg agtggaagcg catcccaata tcgaattggt ccgtgaagaa
      721 gtgaaagaga ttccatcttc gcctgtcatc gtcgccagcg gaccattaac ctcagatagt
      781 ctctccaagt cgattgcggc attgagcggt gatgaacacc tcttcttctt cgatgccatt
      841 tcacccatcg tccgcgccga aagcatcaac atggatatcg ccttccgcgg ttcgcgttac
      901 gacaaaagcg aacaggaaga aggcgattac atcaactgtc cgttcaccaa agaagaatat
      961 tacgaatttt tgactgcctt aaaaacggca gaacgaattg aattgcgcgc ctttgaagat
     1021 gctatcaaaa ccggcgtaaa agcgggtcag ttctttgagg gttgtttgcc agtcgaaatc
     1081 atcgccgagc gtggcgacga atcgctggcg tatggaccca tgcgcccggt cggcttgcgc
     1141 gacccacgca caggcaaacg tccatatgca gtggcacaac tgcggcaaga taatctcgct
     1201 ggaagtttat ataacatcgt cggcttccaa accaatttga aattccccga acaacgccgt
     1261 gtattgcgca tgattcccgg tcttgccaat gcggagtttg agcgctatgg gcaaatgcac
     1321 cgcaacacct tcattgcatc gccgaaactt ttgcgtccca cccttcaaca catcacacgt
     1381 gaagaccttt tcttcgcggg tcaactcacc ggtgtggaag gctacatggg caacatcgct
     1441 acgggcttgt tagcaggtat caacatggca aggctcattc aaaacaaagc cttgctcacg
     1501 cttccgaaag aatcgatgct tggggcgttg tgtcactata tcacccatgc agatttgaaa
     1561 gactttcaac cgatgaaagc caactttgga attttgcctg acttgaatct cggcaagaaa
     1621 atcagcaaac gagaaaaagg gcaagcctac gcagaccgtg cttcacaatc gttggaaact
     1681 tatttacaag aaagttcttg atgaataaac gcgccgtcgt tctttatcta tccatcatcc
     1741 ttggcttggt gttaatcgtc accggatggt acgccgcttc tttcttgacc tatacgtctg
     1801 agtctgttcc tgagggcttt agcggcgacc gcgcctatgc ggacgtgcaa acccaagtgg
     1861 atatgggtcc gcgcacgcct gggtcggcgg gacatgccca aatccgggaa tggatgcgaa
     1921 cagagctaga gtccgctgga tggatagtag aggtccacga gtcagagcgt ctggggcatc
     1981 caatttacaa tatcattgcc aaacgcggaa aagaaccacc acagatcatt ctcgccgctc
     2041 attacgatac gcgcttcttc gcagataatg acccagaccc cgccaagcat acagaacctg
     2101 tccccggcgc aaatgatgga gcttcaggtg tggcagtatt gctagaatta gcacgcaccc
     2161 tgccagcaga tacgacaccc acatggttgg tctttttcga taccgaagac aacggtcgtg
     2221 tagaaggctg ggattggatt ctcggctcac gcgcttttgt agaagaagtg cccattcagc
     2281 cacaagccgt ggtcattatt gatatgatcg gcgatgcaga tttgaacatc ttttacgaaa
     2341 agaattcaga ccccaccatt cgggctgaga tttggtccac agccgccgag cttgggtatg
     2401 gcaaacaatt tattcaagaa gaaaaataca acatgctgga cgaccacacc ccttttgttg
     2461 aaaaaggaat tcctgctgtg gatatgatcg atttcgacta tccctactgg cataccgtgc
     2521 aagacacgcc tgataaggtc tcggcagaaa gcctaaaagc cgtgggcgat acactttggc
     2581 attgggtcgt taaacatggt gaaaaatagt agtagccaaa aggaaagact cggcgtacac
     2641 ttactgtgta tcgcacattt tggcaggtta agtctttctt gcacagacaa cttcaataaa
     2701 aaaacaggct taaaaacgaa ggaataagcg ataagcgaat ggcagtccaa gcatcgttgc
     2761 agaaattaag acccatatgg gtggaaagag tctcccgtga gttggcggct ggagaaggcg
     2821 tgcgctcagg ctttgaagag caattgcaac ggttctatac tctcctcgaa caaactattg
     2881 tcacgggcga tactgcttgg ttagacccca tcttgttcga ttggggacat tctcccacac
     2941 agaccaatct cgaacaagca gattaccacg tctctttcgt gcttgaccgc atgatctcgt
     3001 tgaccatcga tattgcgggc gaccacttgg aagataaaga cgcactcgaa ttgttagcgg
     3061 cggtcatccc cattctagcg cacagcctcg gcattgttgt ccgctatgaa atggaaaccc
     3121 gcgtggcgca catttccaaa gaactaggtt cggttcaaga gaaattacaa aacctcgatc
     3181 agaacaaatc caagtttatt tcggtggcag cgcacgagct aaaaactccg ctaaccctga
     3241 ttgaggggta cgcctccatg atggtggact tggtgcagaa ctctgaacag gcagtgatga
     3301 aaaactacct aagcggagtc aacactggca ttcagcgttt gcgcgacatt atcgatgaca
     3361 tgatcgacgt ctcgttgatc gataaccaac tgctcaccct caacatccaa cctatgtgga
     3421 cgacccatct gctaaacctt gtccgcaatg agttcaagaa gcccatcgcc gaacgtaacc
     3481 agaccctgaa cgtttatgac ttcgatggca gcgatttgat gatttacggc gattcagaac
     3541 gcttgtatca agccttacaa aatgtcgtct ccaacgccat taagtacaca cccgataaag
     3601 ggattatcac g
//
LOCUS       DAOQJE010000282         3607 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_16286_length_3607_cov_6.711149, whole genome shotgun sequence.
ACCESSION   DAOQJE010000282 DAOQJE010000000
VERSION     DAOQJE010000282.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3607)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3607)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3607
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16286_length_3607_cov_6.711149"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..594)
                     /locus_tag="PLT08_08530"
     CDS             complement(<1..594)
                     /locus_tag="PLT08_08530"
                     /inference="COORDINATES: protein motif:HMM:NF027484.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NBR1-Ig-like domain-containing protein"
                     /protein_id="HNE04549.1"
                     /translation="MYRRNYKILYFLIGLTLMLACVPTLGPAPAPIPTFDVNAPLTAI
                     VLTANSAATQTALFLPPTATPTAPTNTPFPTATPLPTFLFLIPTKDEPPTQIPLGNSD
                     KAYECQVLSAEPRDPLPVSASFVAKWLVANIGKSTWDANNADYRYIDGYRLHLQSVYD
                     FPVNVAPGVTVELTVDMQAPATPGQYSTFWIIYIGKNA"
     gene            complement(605..1231)
                     /locus_tag="PLT08_08535"
     CDS             complement(605..1231)
                     /locus_tag="PLT08_08535"
                     /inference="COORDINATES: protein motif:HMM:NF027484.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NBR1-Ig-like domain-containing protein"
                     /protein_id="HNE04550.1"
                     /translation="MLKKTLILLSFAILLVACLPSQQPTVDAQAQINTAVAGTMQVNR
                     QIDQAVQQTVAAKEAVDAPKATQNIIDINNPGLQVNETATPTETPFVLASPTATASPI
                     PPKYSCSVYKKKPKDNEYFSKGEEFDVKFIVTNTGTRTWPKGIDFKFVSGTQLAAPKR
                     VEIPVTLAPWQTYEIVLDGKAPAEKGFYVMTFFVDGPMCYGYIAINVK"
     gene            complement(1314..2645)
                     /gene="ablA"
                     /locus_tag="PLT08_08540"
     CDS             complement(1314..2645)
                     /gene="ablA"
                     /locus_tag="PLT08_08540"
                     /EC_number="5.4.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050066 - lysine 2,3-aminomutase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0046440 - L-lysine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine 2,3-aminomutase"
                     /protein_id="HNE04551.1"
                     /translation="MKTEFVSKRAKIYADVPDEKWNDWRWQLSNRLNSVEDIEKVLPL
                     TDSERKALQTQGLFRVDVTPYFISLIDPNDPNDPTRLQIIPRAEEMQAFTAMMEDSLA
                     EDRHSPVPGLVHRYPDRVLMLVTTQCASYCRYCTRSRIVGDPGQTFKREEFEMQIEYL
                     KRTPQVRDVLLSGGDPLVLAPKVLEELLSRLREIPHIEIIRIGSRVPVFMPMRVTQEL
                     CDMLAKYHPLWMNIHVNHSNEISKELAEATDRLTRAGIPVGNQSVLLAGVNDNVHIQR
                     QLVQDLVRIRVRPYYLYQCDLVEGAGHFRTPVAKGIEIMEGLRGHTSGYAVPQYIVDA
                     PGGGGKIPVMPNYMLSMSDHKVILRNYEGYVTTYEEPTDYIPANAAKFKGLKRPEPGQ
                     GGLTALLDGEELFIKPEGFDELHNRDGIQHRLKDDNKWKPLGIGSGETDND"
     gene            2851..>3607
                     /locus_tag="PLT08_08545"
     CDS             2851..>3607
                     /locus_tag="PLT08_08545"
                     /inference="COORDINATES: protein motif:HMM:NF012703.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginase family protein"
                     /protein_id="HNE04552.1"
                     /translation="MTEEGTHGYWGDLHRPDLTPEQTDFSIIGIPFDGLASARKGAAL
                     APERLRYWSRHLTPFSEDRTRLKDMTICDLGDIEIKNPETDFVTVRQRVATLPNMPII
                     LGGDHSVTIPVLQAQRERYKDQRLGILWIDAHPDLCDFFDGSRLSHANTMRRALDFGI
                     EAHDVCMVGLRSWEEQEIDLIENGGIHVYTAADVAERGIRHIADSIYNILNDCDAVHV
                     SLDIDCLDPSVAPGTGIPEFGGLTSRDVLTLIKS"
BASE COUNT          933 a          827 c          903 g          944 t
ORIGIN      
        1 cgcatttttg ccaatgtaaa taatccaaaa tgtggaatat tgtcctggtg tcgctggcgc
       61 ttgcatgtcc acggtcaatt ctactgtaac tcctggagca acatttacag ggaaatcgta
      121 tacagattgc aaatgaagtc tgtatccatc tatataacgg taatcagcgt tatttgcgtc
      181 ccatgtggac tttcctatat tagcaacaag ccatttagca acaaaactcg ctgaaacggg
      241 aagcgggtct cgcggctcag ccgaaagaac ctgacattca tatgctttat cagagttacc
      301 aagcgggatc tgagtaggcg gctcatcttt tgtaggaata agaaaaagga aagttggtaa
      361 aggtgtggct gtggggaaag gtgtattagt aggggctgta ggtgttgcag taggaggcaa
      421 aaacaatgct gtttgggtcg cagcagaatt tgcagttaat acaatagcag ttagtggagc
      481 attgacgtca aatgttggaa taggcgcggg ggcaggtcct aatgttggca cacacgccaa
      541 cattagggtt aagccaatta aaaaatataa tattttgtaa ttacgcctat acattctttt
      601 attcctattt cacattgatt gcaatatagc cataacacat aggaccgtcg acaaaaaagg
      661 tcatgacata gaatcccttt tctgctggtg ctttcccatc aaggacaatc tcataagttt
      721 gccaaggcgc aagcgtgact gggatttcta ctctctttgg agctgcaagc tgagtcccgc
      781 taacaaactt aaagtcaatc cctttaggcc atgtgcgtgt ccctgtattt gtgacaatga
      841 acttaacatc aaactcttcg cctttactaa aatactcatt atctttgggc tttttcttat
      901 atacagaaca tgaatatttt ggtggaatag gagaggctgt tgctgtagga gaagccagaa
      961 caaaaggagt ttctgttggg gtagcagttt cattaacttg taagccagga ttgttaatgt
     1021 ctataatatt ttgagtggct ttgggggcat ctactgcttc ttttgcagca actgtctgtt
     1081 gaactgcttg atcaatctgg cgattaactt gcatggttcc ggcaacagcg gtattgatct
     1141 gtgcctgtgc atcgaccgta ggctgctgag aaggcagaca tgccactagc aggatggcga
     1201 aagatagcaa aatgagtgtc ttttttagca taacaaactc cttaatttta atagacctga
     1261 cggagatttt ctcccgtcag gtctaagtat atcagcccct gtaaattggc gtattaatcg
     1321 ttgtcggtct cgccagagcc gattcccaac ggtttccact tgttatcatc tttcaagcgg
     1381 tgttggatgc cgtcacgatt gtggagttcg tcgaaacctt caggcttgat gaacagttct
     1441 tcgccgtcga gcaatgctgt cagaccgcct tgacctggct cagggcgttt gagcccctta
     1501 aacttggcgg cattggcagg gatgtaatcc gtcggttctt cgtaggtggt cacgtaccct
     1561 tcatagttac gcaagataac cttatggtca gacatgctca acatatagtt cggcatgact
     1621 ggaatcttgc caccgcctcc gggcgcatcg acgatatatt gcggcacagc atagccagag
     1681 gtatgtccgc gtagaccttc catgatttcg atacctttgg caaccggcgt acggaagtga
     1741 ccagcacctt ccacaaggtc gcactggtaa aggtagtatg gacgcacacg aatacggacg
     1801 aggtcttgca ctagctgacg ttgaatatgg acgttatcat tcacgcctgc caataacaca
     1861 gactgatttc caaccgggat acctgcacga gtaaggcgat cggttgcttc agcaagttct
     1921 ttgctgatct cattcgagtg gttgacgtga atattcatcc acaaaggatg gtatttggcg
     1981 agcatgtcgc acaattcttg tgtgacgcgc atgggcataa agaccggcac gcgtgaacca
     2041 atgcggataa tctcaatatg cgggatctcg cgcaaacggg aaagtaattc ttcaagaact
     2101 ttcggcgcga gcacgagcgg atcgccgcca gagagcagca cgtcacgaac ctgtggcgtg
     2161 cgtttgagat actcaatctg catctcaaat tcttcgcgtt tgaaggtttg cccggggtca
     2221 cccacaatgc gggagcgagt gcagtaacgg cagtacgagg cgcactgagt cgttacgagc
     2281 atgagaactc tgtcaggata ccggtgaaca agccccggca cgggagagtg gcggtcttcc
     2341 gcaagggaat cctccatcat ggcggtaaag gcttgcatct cctctgcacg tggaataatc
     2401 tgcaaacgag tcgggtcatt cgggtcgttg gggtcgatca acgaaatgaa atagggagtc
     2461 acatccacac ggaatagtcc ctgtgtttga agagcctttc gttcgctatc ggtaagcgga
     2521 aggacttttt cgatatcctc aacagaattg aggcggttgg aaagctgcca gcgccagtcg
     2581 ttccactttt cgtcgggaac atcggcgtaa atcttagcgc gtttggaaac aaactcggtt
     2641 ttcatggttc ctccattagt tgtaaataga tcagaatatc aacattaaaa aaagccactc
     2701 acattggagg gtcgtggtcg gggaggtaaa aacctgctat gcccataaag gtgacgttct
     2761 tcggcataat tgcattttac gggcaaacga taggggggtc aatgcgggta gttttcaaag
     2821 gtaaaatagc caaatccaag gagcataacg atgaccgaag aaggtacaca cggctactgg
     2881 ggcgacctac accgcccaga cctcaccccc gaacagaccg atttcagcat tattggaatt
     2941 cccttcgatg ggcttgccag cgctaggaaa ggagcggctc tcgctcccga acgcctgcgt
     3001 tactggtcca ggcatttgac tccctttagc gaagaccgca ctcgcctgaa agatatgacg
     3061 atctgcgatt tgggtgatat cgaaatcaaa aaccccgaaa ccgattttgt caccgtgcgt
     3121 caacgcgttg cgacattgcc aaacatgccc atcattctcg gcggtgacca ttctgtgaca
     3181 atccctgttt tacaagccca acgtgaacga tacaaagacc aacgcctcgg cattttatgg
     3241 atcgatgctc acccagacct atgcgatttc ttcgatgggt cacggctttc tcatgccaac
     3301 accatgcgcc gtgccttaga ctttggcatt gaagcccatg atgtatgtat ggtggggttg
     3361 cgctcttggg aagaacaaga aatagactta atcgaaaatg gcgggattca tgtgtataca
     3421 gctgctgatg ttgccgagcg tggcattcgc catattgcgg atagtattta caacatctta
     3481 aatgactgtg atgccgtcca tgtttcactg gatattgact gcctcgaccc atccgttgcg
     3541 ccgggcacgg gcattcctga attcggcggg cttacttcac gcgatgttct aacgttgatt
     3601 aagtcga
//
LOCUS       DAOQJE010000283         3601 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_16334_length_3601_cov_4.460519, whole genome shotgun sequence.
ACCESSION   DAOQJE010000283 DAOQJE010000000
VERSION     DAOQJE010000283.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3601)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3601)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3601
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16334_length_3601_cov_4.460519"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..250
                     /locus_tag="PLT08_08550"
     CDS             <1..250
                     /locus_tag="PLT08_08550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE04553.1"
                     /translation="ETGRPVLYMGGFSGGDAVVTVDDLKELVANGELRYILYGGDRGN
                     QEITNWLSSSCAIVNDFNNLSQAQQGPQNQAMTLYSCR"
     gene            complement(282..611)
                     /locus_tag="PLT08_08555"
     CDS             complement(282..611)
                     /locus_tag="PLT08_08555"
                     /inference="COORDINATES: protein motif:HMM:NF017273.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HNE04554.1"
                     /translation="MSALSIWLLFVAIGAGTFTLRFLFIYLFGKVEMPTWLSRALRFV
                     PAAALAALVFPALTHPAGHLDISLTNFRLLAGLGGALVAWRTRNVLLTILVGMALLWS
                     LQAAFGM"
     gene            complement(608..1309)
                     /locus_tag="PLT08_08560"
     CDS             complement(608..1309)
                     /locus_tag="PLT08_08560"
                     /inference="COORDINATES: protein motif:HMM:NF015549.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HNE04555.1"
                     /translation="MKWNKDFIAGIKGAAPILIGVIPFAMITGVGAVSVGLSFWESMG
                     MSVFVFAGASQLVVFQLMSVGTPWIIMVLTAWVVNVRFTMYSATLAPYLQKLSTGRKA
                     LLAYMLSDQAFGVSMSRFISTDKEIDYRWYYFGAALIIWLAWQVSAVIGTLLGALVPA
                     SLGFDFAFPLSFMALMFGALRDRPTVVAALTGGFIAVLAKSLPYNTGLVLATFLGIIA
                     GYVAESLKLKEEAVL"
     gene            1480..2391
                     /locus_tag="PLT08_08565"
     CDS             1480..2391
                     /locus_tag="PLT08_08565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE04556.1"
                     /translation="MMKRTIALTLSFLWLTACVTLTNPTPLTPTETVPTTPSVSAADN
                     TPTSTPLINNSTLLYISGTTHIESKPQSWPNIDAFLNFLQNITAQGLKWSVGADIGWL
                     EGEPRAAELIQASEALGVQWDIHAHNPQERAKAAYLITQLGGHPTSVISGMLVSEFSS
                     IGPQTYQGFTWTPHVLWGGTNCPGHRPGCDDLAAGIWIPLSAEQYTTHDPNGTLIRVG
                     GGSHQLADGLVLAESIANGQYPYPIISFTLMVSPTSLTIVNSNDGLEQITTFANKLKQ
                     YSFVHWAYIEETAQAWTKAGKVPSRIE"
     gene            complement(2431..>3601)
                     /locus_tag="PLT08_08570"
     CDS             complement(2431..>3601)
                     /locus_tag="PLT08_08570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE04557.1"
                     /translation="SRGIPMFYMYYSRIELILRYRYGIGGDAYTLPTREVVSGVSPRS
                     RVELLHQEKDGCKSFPCSITNHVTHNIITSFVSLPASLTFKDTYNIVKSDLPYWNKGW
                     RDGQIDIGGWVAIIFSLVLIALGAAAIWRQSRAGVWLPAFFFLAYLLTNSIGLTSGGR
                     YVTPVDWILYFYFAAGAVQLLYWLLAMTGYIAEIEPANESYGIVPVASVINIRALVPI
                     IAGILGIGVLIPLPEFLFQPRYQAQTPSETFASLRETGLFERSNFSEEELTAFLAEPG
                     AMILTGRALYPRYYPAGEGESDLNTHYKKFHFQRLVFTVIGPDFPGAIGLVIPGDSLP
                     KGLSLQAADVVVLGCWTPDRYSPLLNAVVVFVTSGDGYVYNRSPESPLHCPLKIP"
BASE COUNT         1108 a          928 c          736 g          829 t
ORIGIN      
        1 cgaaaccggg cgcccggtct tatatatggg tggtttcagt ggcggtgatg ccgttgtaac
       61 agtagatgat ctgaaagaat tagttgccaa tggagagttg cgttacattc tctacggcgg
      121 cgatagaggc aaccaagaaa ttaccaattg gctaagtagc tcatgtgcga ttgtgaatga
      181 tttcaataat ctatctcaag cgcagcaggg tccacaaaat caagccatga ccttatactc
      241 ctgcagataa taaaaagccc cgagataatc tcggggcttt tttacatccc aaatgctgct
      301 tgaagagacc acaacaaagc catgcccacc aaaatggtaa gcagaacatt tcttgtgcgc
      361 cacgccacca atgccccacc aagccctgcc aagagacgga aattcgtgag cgaaatatca
      421 aggtgccccg cagggtgagt caacgctggg aagaccaacg ccgccaacgc tgccgcaggg
      481 acgaacctta gagcccggct caaccaagtg ggcatctcaa ctttgccaaa gagataaata
      541 aacaaaaacc gaagcgtaaa tgtgcccgct ccaatcgcca caaagagaag ccaaatcgat
      601 aatgcgctca taggactgcc tcctctttta gcttcagact ttcagcaaca tacccggcaa
      661 taattccaag aaaggttgcc aataccaacc cagtgttata tggcaagctt ttcgccaaca
      721 cggcgataaa accgccggtc aatgctgcta ctactgtagg gcggtctcgc aaagctccga
      781 acatcaacgc catgaaactg agcggaaagg caaagtcaaa gccgaggctg gcgggtacaa
      841 gagcacctaa caatgtccca atcacagcgc taacttgcca tgccaaccag atgatcagcg
      901 cagcgccaaa gtaataccac cgatagtcta tttctttatc ggtggaaata aagcggctca
      961 tactgacccc aaatgcctgg tctgagagca tatatgccaa caatgcttta cgtcccgtag
     1021 aaagtttttg taaatatggc gctaatgtag cactgtacat cgtaaaacgt acatttacca
     1081 cccacgccgt taaaaccata ataatccatg gggtgcctac actcatcagt tgaaacacca
     1141 ccaactgaga agcccccgca aacacaaaca cagacatccc catcgactcc caaaatgaca
     1201 aaccaacact caccgctccc actccagtga tcatcgcaaa aggaatcact ccaatcaaga
     1261 ttggggctgc cccttttatt ccagctataa aatctttatt ccatttcata atttatccat
     1321 actgtatttt ttagggggca attctacccc tgaactctac ggttttttac aatctattta
     1381 caactctatg atagctgtca cgattaattc catgcaagca tgatgcgagt cactcttact
     1441 aaatttttca aaatcataga atctacctta aaggagaata tgatgaaacg aacgattgcc
     1501 ctcactctta gttttttatg gctcacagca tgtgtaaccc tgacgaatcc tacgcctctt
     1561 actccaacgg aaactgttcc aacaacccca agtgtctctg cagccgacaa tactcccaca
     1621 tccacgccac ttatcaataa cagcacatta ctctatattt ccggcactac tcacattgaa
     1681 tccaagccgc aatcatggcc aaatatagat gcatttctaa atttccttca aaacattaca
     1741 gcacaaggtt tgaaatggtc agttggagca gatataggct ggctagaagg agaaccgcgt
     1801 gctgctgaat tgatccaagc ctctgaagca ttgggggtgc aatgggatat ccatgcccat
     1861 aacccacaag agcgtgccaa agcagcttat ctcatcactc aattaggcgg gcaccctact
     1921 tcagtcatat ctgggatgct tgtcagtgaa ttcagtagca tcggacctca aacataccaa
     1981 ggtttcacat ggactccaca cgtgttatgg ggtggcacga actgtcccgg tcaccgccca
     2041 ggatgtgatg atctagcggc aggcatctgg attccactta gtgctgagca atacaccact
     2101 cacgacccca atggtacact catccgcgtt ggcggtggct ctcatcaatt agccgatggg
     2161 ctagttctcg ctgaatctat cgcaaatgga caatatccat atccaattat cagtttcaca
     2221 ttaatggtct ctcccacctc tctaaccata gtcaacagca atgatggttt agaacaaatc
     2281 acaacatttg caaacaaact gaaacagtat tcatttgtcc attgggcata tattgaagag
     2341 acagctcaag cttggactaa agccggaaag gttcccagtc gaattgagtg aaataaaaac
     2401 aggtcacgtc ttataacgtg acctgtttta tcaaggtatt tttaatggac agtgcaacgg
     2461 tgactctggt gaacggttgt atacatatcc atcgccagag gtaacaaaaa ccacaactgc
     2521 attcaggagc ggggagtatc gatccggtgt ccagcatcca agcacaacca catctgccgc
     2581 ttgtagtgaa agcccttttg gtagagagtc acccggaatc actagtccaa ttgcaccagg
     2641 aaagtccggt ccgatcacgg taaacactaa tctttgaaaa tgaaattttt tgtaatgtgt
     2701 atttaagtca gattcgcctt cacccgccgg atagtagcgc ggatataaag cccgcccggt
     2761 caagatcatc gcacctggct cagcaagaaa agcggtcaac tcttcctccg agaaattgga
     2821 acgttcgaaa agtcccgttt cacgtaaaga agcaaaagtc tcagatggag tctgtgcctg
     2881 atagcgtggt tgaaacaaga attctggcag gggaattaac acgcctattc ccaaaatgcc
     2941 cgcaataatc ggtacaagag ccctaatatt aattacactt gcaactggaa caatgccata
     3001 cgattcgttc gcaggttcta tttctgcaat ataccccgtc atcgccagaa gccagtaaag
     3061 caattgcaca gcacctgccg caaaataaaa atacaagatc caatccaccg gcgtgacata
     3121 acgcccgcca gaggtaagcc caatcgaatt tgtcaaaaga taagccagga agaaaaaggc
     3181 gggcaaccag accccggcac gactctgcct ccaaatagcg gcagcaccca atgcaatcaa
     3241 cactaaggaa aagatgattg ccacccatcc gccaatatct atttgaccat cacgccagcc
     3301 cttgttccaa tacggcaaat ccgatttcac aatattgtat gtatctttaa aggttaacga
     3361 ggcaggcagt gatacaaatg aggtaatgat gttatgagta acgtgattgg taatcgaaca
     3421 tggaaatgac ttacagccat ccttctcctg atggagaagc tccactcgag agcgcggtga
     3481 tacgcctgaa acaacttctc gggttggcag agtgtaggca tccccaccga ttccataccg
     3541 ataacgtagt atcaattcaa tacgagagta gtacatgtaa aacatgggaa taccacgaga
     3601 t
//
LOCUS       DAOQJE010000284         3599 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_16356_length_3599_cov_5.452596, whole genome shotgun sequence.
ACCESSION   DAOQJE010000284 DAOQJE010000000
VERSION     DAOQJE010000284.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3599)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3599)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3599
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16356_length_3599_cov_5.452596"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            14..1285
                     /locus_tag="PLT08_08575"
     CDS             14..1285
                     /locus_tag="PLT08_08575"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HNE04558.1"
                     /translation="MTILSTKSTTAWAEVDENGRLILPPEVAQRYGLHPGSKVRLDEG
                     NNIVRMHRPVTHLTKIYIEPTVACNLDCITCFRNAWDQPIGKMTDETFESIFNSLKQM
                     DPIPSVYFGGIGEPLFHPKTIEWIRRIKQELGVKVELITNGTTLTERKTRELIDAGLD
                     ILWVSLDGATPESFSDVRMGAELPVILENLRRLFRMRNGGHFPQPEIGVAFVAMKRNI
                     NDLPKVIKLGHTFGARYFSVSNIQPATEGMQEDRLYKRTIRNIAYLPSPVLPKLSLPK
                     MDFNEETQAALTEAFNSGCNVSFAGNNWGGANDVCNFVESGTMSIAWTGEVSPCWPLM
                     HTHMSYLHGKPRLSKKHVIGDTRKQSLEEIWLSPEYVAYRERLHNFVFAPCTFCGGCD
                     LSETNEEDCLGNDIAPVCGGCLWAQGIIQCP"
     gene            1364..1696
                     /locus_tag="PLT08_08580"
     CDS             1364..1696
                     /locus_tag="PLT08_08580"
                     /inference="COORDINATES: protein motif:HMM:NF014636.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoaD/ThiS family protein"
                     /protein_id="HNE04559.1"
                     /translation="MQLRFYANMRTTVGMPLLEINEAMVDSFRKLIPLLLEQYPETAF
                     HLLDEHGELRPDVPVYVDGRNPRLSSVGMDTPLKPDSVISFFSPISSGKLNVEVLREP
                     TIDTRSEP"
     gene            1693..1971
                     /locus_tag="PLT08_08585"
     CDS             1693..1971
                     /locus_tag="PLT08_08585"
                     /inference="COORDINATES: protein motif:HMM:TIGR01687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoaD/ThiS family protein"
                     /protein_id="HNE04560.1"
                     /translation="MKVNFFATLRDIAGGKTVEFDLDHGITARELLDAICERFPAMKK
                     ELLKEDGEMHGHVHFFINGRDVQFTDLQFATPITQGDTVNVFPAVGGG"
     gene            2113..3117
                     /gene="moaA"
                     /locus_tag="PLT08_08590"
     CDS             2113..3117
                     /gene="moaA"
                     /locus_tag="PLT08_08590"
                     /EC_number="4.1.99.22"
                     /inference="COORDINATES: protein motif:HMM:TIGR02666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP 3',8-cyclase MoaA"
                     /protein_id="HNE04561.1"
                     /translation="MTILDTLNRPLRDLRISVTDRCNFRCVYCMPKEIFGSDYKFLHH
                     SQVLTFEEITRIARLFAGLGVKKIRLTGGEPLVRKDFPDLVALLAQIPDLDLTMTTNG
                     SLLPRFAEQLKKSGLNRVTVSLDSLDNKIFKSMNDVDFPVEKVLEGMDAAKAAGLEPI
                     KVNMVVKRGVNEDSILPMARFFREKGFILRFIEYMDVGSTNGWRMNDVVSAKEIISII
                     NAEMPLEPADPNYRGEVAERWRYKDGQGEIGVIASVTQAFCRDCNRARLSAEGQLYTC
                     LFAVKGHDFRDMLRNGASDEEILHKIETVWGKRTDRYSEIRSDNTIPLPKVEMSHIGG
                     "
     gene            complement(3198..>3599)
                     /locus_tag="PLT08_08595"
     CDS             complement(3198..>3599)
                     /locus_tag="PLT08_08595"
                     /inference="COORDINATES: protein motif:HMM:NF013481.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016625 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, iron-sulfur
                     protein as acceptor [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde ferredoxin oxidoreductase C-terminal
                     domain-containing protein"
                     /protein_id="HNE04562.1"
                     /translation="PLAWKGKGEMNFIFQNVHAVTDCLDICKFATFAESLDSFAAQYE
                     AMTGVPCDAGHLLKVGERVYNLERHINNLNGFREGSDTLPKRFTSEPSTYGGSKGQVC
                     ELDDMLVEYYEKRGWVNGVVPESKLKELEII"
BASE COUNT          906 a          906 c          882 g          905 t
ORIGIN      
        1 gtaaggagaa cgtatgacca tcctctcgac aaaatccacc accgcctggg cagaagtgga
       61 cgaaaacgga cgcctcattc ttccgcccga agttgcccag cgttacgggc tgcatcctgg
      121 ctcgaaagtt cgcctcgacg aaggcaacaa catcgtccgc atgcatcgcc cggtgactca
      181 cctcactaaa atttacatcg aacccactgt cgcctgcaac ctcgactgca tcacctgctt
      241 ccgcaatgcc tgggaccagc ccatcggcaa aatgaccgat gaaacctttg agagcatctt
      301 caacagcctc aaacaaatgg acccgattcc cagcgtctat ttcggcggca tcggcgaacc
      361 cctctttcat cccaaaacca tcgaatggat ccgccgcatt aagcaggaac tcggcgtaaa
      421 agtagaactc atcaccaacg gcaccaccct caccgaacgc aagacccgtg agttgatcga
      481 tgccggtctg gatattctct gggtgtctct agatggcgct acccctgaaa gcttctcaga
      541 tgtacgcatg ggcgctgaac tccccgtcat tctagaaaac ctgcgccgac tttttagaat
      601 gcgtaatggc gggcacttcc cacagccaga aattggcgtc gcctttgtgg cgatgaagcg
      661 taacatcaat gatctgccca aggtcatcaa acttgggcac accttcggtg cccgttactt
      721 ctcggtcagc aacattcaac ccgccacaga gggcatgcaa gaagatcgtc tctataaacg
      781 caccatccga aacattgctt acttaccttc gcccgtcttg cccaaactca gtctgcccaa
      841 aatggacttc aacgaagaaa cccaagccgc tttaacagaa gccttcaata gcggatgcaa
      901 cgtcagcttc gcgggcaaca actggggcgg tgcaaatgac gtctgcaatt tcgttgaaag
      961 cggcaccatg tctatcgcat ggacggggga agtcagcccg tgctggccct tgatgcacac
     1021 gcacatgagc tatttgcatg gcaaaccgcg cctttcaaag aaacatgtta tcggcgatac
     1081 tcgcaaacaa tcactggaag agatctggct cagcccagaa tatgtcgcat atcgcgaacg
     1141 cctgcacaac tttgtattcg caccctgtac cttttgcggc ggctgtgacc tctctgaaac
     1201 caacgaagag gattgcctcg gcaatgatat cgccccggtc tgcggcgggt gcctctgggc
     1261 gcagggcatt attcaatgcc cgtaatttga tctagggttt tcaaagagct cgggtcttcc
     1321 gagttctttg ttttaacacg aacgaaaatg ataaaatcag aacatgcagc tacggtttta
     1381 tgccaatatg cgaaccacag ttggaatgcc actgctcgaa atcaacgaag cgatggtcga
     1441 ttcatttcgc aaactaatac cgctactcct cgaacagtat cccgaaactg cattccattt
     1501 actggatgaa catggcgaat tacgcccgga tgttcccgtc tatgtggatg gaagaaaccc
     1561 gcgtctttcc tcggttggaa tggatactcc tctcaaaccg gattctgtca tcagcttttt
     1621 ctcccccatc tcaagcggaa aactaaatgt agaagtgtta cgtgagccga ctattgacac
     1681 aaggagtgaa ccatgaaagt aaatttcttt gcgaccttac gtgatattgc tggtggtaaa
     1741 actgttgagt ttgaccttga ccatggcatt actgcccgtg aattgttgga tgccatttgt
     1801 gaacgcttcc cagcaatgaa aaaggaatta ctcaaggaag atggcgagat gcatgggcat
     1861 gtccacttct ttattaatgg gcgggatgta caatttactg acctgcaatt tgccacgccg
     1921 atcacacaag gcgatactgt gaatgttttc cccgcagtgg gcggcggtta attcaggcaa
     1981 atttcgaaat tgaaaaagct tagaagcgcc gtatcagacc tgagattaag aaagatgcgg
     2041 tttttaggtc aaaaacaaca tatccttacg ggtataatcc tttatgtcag agcttgttga
     2101 cactcaccta ttatgacaat tctcgatacc ctcaaccgtc ctcttcgtga cttgcgcatc
     2161 tcggtcactg atcgctgtaa ttttcgctgt gtatactgca tgccgaagga aatctttggc
     2221 tcagattata agtttctgca tcacagccag gtgctgactt tcgaagagat tacgcgcatt
     2281 gcacgtttgt ttgctggctt gggggtcaag aagattcgcc ttactggggg cgaacccctt
     2341 gtacgtaaag attttcctga ccttgtggca ttactggcac agatccccga ccttgatctc
     2401 acgatgacaa ccaacgggtc gcttctgcct cggtttgccg agcaactcaa aaagtctggg
     2461 ttgaatcggg taacggtcag ccttgattct ttggataaca agatattcaa gtcgatgaac
     2521 gatgtggact tcccagtgga gaaagttctt gaaggcatgg atgctgctaa agccgctggg
     2581 ttggagccga tcaaggttaa tatggtggtg aagcgcggtg tgaatgaaga cagcattttg
     2641 cccatggcgc gcttcttccg tgaaaaaggt tttattctgc gtttcattga atacatggat
     2701 gtggggagta ccaatggctg gcgtatgaac gatgtggttt cggcaaaaga gatcattagc
     2761 atcatcaatg ctgaaatgcc gctagagcct gctgacccga attaccgcgg agaagtggca
     2821 gaacgctggc gttataaaga tgggcagggt gaaattggtg tgatcgcttc ggtgacacaa
     2881 gccttttgcc gtgattgcaa tcgtgcgcgt ctttctgcag aagggcaact ttatacctgt
     2941 ctcttcgcag tcaaagggca tgacttccgc gatatgcttc ggaatggtgc cagtgacgaa
     3001 gagatcttgc ataagatcga aactgtttgg ggtaaacgca cggaccgata ttctgaaatt
     3061 cgttctgata acaccattcc gcttccaaaa gtagaaatgt ctcatattgg cggttaggcg
     3121 ttttcattct ctattgttct taacataaaa aagacctccc gtaaaatacg ggaggtcttt
     3181 tttattcaac ttgtgaacta gatgatttcc agttctttga gtttggattc gggtacaacg
     3241 ccgttcaccc atccgcgctt ctcgtagtat tccacgagca tatcgtcgag ttcgcagacc
     3301 tgacccttgc tgccgccgta ggtggagggt tcgcttgtga agcgtttcgg caaagtatct
     3361 gaaccttcgc ggaagccgtt gaggttgttg atatggcgtt cgaggttgta gacgcgttca
     3421 ccaactttga gaaggtgacc agcatcacac ggcacgcccg tcatcgcttc gtattgagcg
     3481 gcgaaggagt cgagggattc ggcgaaggtg gcgaatttgc agatgtccaa gcagtcggta
     3541 acggcgtgaa cgttctggaa gatgaagttc atttcgcctt tgcccttcca cgcgagcgg
//
LOCUS       DAOQJE010000285         3596 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_16375_length_3596_cov_5.454391, whole genome shotgun sequence.
ACCESSION   DAOQJE010000285 DAOQJE010000000
VERSION     DAOQJE010000285.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3596)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3596)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3596
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16375_length_3596_cov_5.454391"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..308
                     /locus_tag="PLT08_08600"
     CDS             <1..308
                     /locus_tag="PLT08_08600"
                     /inference="COORDINATES: protein motif:HMM:NF020123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HNE04563.1"
                     /translation="KAEMPAIEKMYNEYKDQGFNVLAINSTFQDDPLQIKPFTEEYSL
                     TFPILLDETSDISRDYQVRSLPSSYFVNRYGIITEVVIGGPMSEALLRTKIEEALKP"
     gene            305..1033
                     /locus_tag="PLT08_08605"
     CDS             305..1033
                     /locus_tag="PLT08_08605"
                     /EC_number="2.4.99.-"
                     /inference="COORDINATES: protein motif:HMM:NF013915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HNE04564.1"
                     /translation="MIETFQNLFAPPRHMILLVIAAWLGLALAERRTERHGISKEDLN
                     NITFYGLIAFVIGGRISYITQNISAFTKSPLGIVSINPNLFDPIGAIAVAFIVLIVYG
                     QRKQLNIWNIFDSLTLFFAVLAIGLGLSHLAAGSAFGLPTDLPWGINLWNAARHPTQI
                     YDTLASSLILILLWLFKPSPRPGITFLTYAAFVALAQLILQAFRANYTIIFSGLRQEQ
                     VVAWVGLLVCFILIEVRLKSQEKQ"
     gene            complement(1077..1640)
                     /gene="raiA"
                     /locus_tag="PLT08_08610"
     CDS             complement(1077..1640)
                     /gene="raiA"
                     /locus_tag="PLT08_08610"
                     /inference="COORDINATES: protein motif:HMM:TIGR00741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_component: GO:0022626 - cytosolic ribosome [Evidence
                     IEA];
                     GO_function: GO:0045182 - translation regulator activity
                     [Evidence IEA];
                     GO_process: GO:0009386 - translational attenuation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HNE04565.1"
                     /translation="MSNKVEVQTRHIRLTERIEEYVSKKAGNLDHYLPAIEDARVELS
                     HHQAARDVNDRNVAQITVFGKGFTLRSEERADEALAAFDKALDNMQRQIERHKGKKYH
                     GRGDGRSAAEAVDDIIDDETGQLSPLFAKRKKFVMYPMNEEEALVQMRNLGHDNFFVF
                     YNAETSKINVLYRRRNGSYGLIEPELG"
     gene            complement(1682..2407)
                     /locus_tag="PLT08_08615"
     CDS             complement(1682..2407)
                     /locus_tag="PLT08_08615"
                     /inference="COORDINATES: protein motif:HMM:NF012383.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HNE04566.1"
                     /translation="MYNLKYKAYRSIWSGLDLLFPPECGGCGKPGTRWCDICQSSLKL
                     LDGIVCDVCGLPQDKPGVCSTCLANRPRFHSLRAWTVFEKPIQPALHKLKYRRDFSMG
                     DAIAAAMLPFVQSLNWNIDLVIPIPLGKQRMHERGYNQVAMIAKPLAMGLGLQYSPHA
                     LARRKETRSQVGLSREERQKNVHDAFQASGVQGKIALIMDDVSTTGSTLSSGAEALYA
                     AGAKEVYALTVARALSHHDLRHV"
     gene            complement(2459..3424)
                     /locus_tag="PLT08_08620"
     CDS             complement(2459..3424)
                     /locus_tag="PLT08_08620"
                     /inference="COORDINATES: protein motif:HMM:NF012694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HNE04567.1"
                     /translation="MGIAIAIVVGIALFGSIVFVVVIGARYAREAQGDESDPVLSRLA
                     EATQRGENVSLEDIELQQPFMERVVIPVAKRLGELSTRFTPDKLLQETALKLELAGNP
                     GRIDASTFLATRFVGAAVFGGLLLLISNLPTVNWPFGRVFLVVSIFTLLGFFFPQLWL
                     GSRISRRQNEVRKALPDALDLLTICVEAGLGFEAAMSKVADKWENELSLMFGRCIREI
                     QLGKTQREALRDMADRIGLPELTSFVAAVIQSQLLGVSMAKVLRIQSDQMRLKRRQRA
                     EEEAHKAPVKMIIPMALLTFPSIMIILMAPAGFQIAGAFGPFIGN"
BASE COUNT         1051 a          904 c          669 g          972 t
ORIGIN      
        1 gcaaagccga aatgcccgct attgaaaaaa tgtacaacga atacaaggat cagggcttta
       61 acgtgttagc gatcaacagc acctttcaag atgatccgct tcagatcaaa ccgttcactg
      121 aagaatatag cttaaccttt cccatcttat tagatgaaac ttcagatatc agccgtgatt
      181 atcaagtccg ctcactgcct tcatcgtatt ttgtcaatcg ctatggcatc atcaccgaag
      241 ttgtaatcgg tggtcccatg tccgaggctt tgctccgcac taaaattgaa gaggctttaa
      301 aaccatgatc gaaacgttcc aaaatttatt cgcgccgcca cgtcatatga tcttgctcgt
      361 tatcgccgca tggcttggtc ttgcattggc agaacggcgt acagaacgtc acggcatcag
      421 caaagaagac ctcaataaca ttaccttcta tggattaatt gcttttgtca ttggtgggcg
      481 catttcgtat attacccaaa acatatcggc gtttacaaaa agtccacttg gcattgtttc
      541 catcaaccca aatctcttcg accctatcgg tgcaattgct gtagcattta tcgttcttat
      601 agtatatggt caacgcaagc aactcaacat ttggaatatc tttgactcgc tgacactttt
      661 tttcgctgtt ctcgccattg ggcttgggtt aagccacctt gctgcgggca gtgcatttgg
      721 actccctacc gatcttccat ggggaatcaa cttatggaat gccgcacgtc atccaactca
      781 aatttatgac acgctcgcct cttccctcat attgatcttg ctctggctct tcaaaccaag
      841 tccacgccca ggcatcacct tcttaaccta tgctgcattt gtagccttag cacaactcat
      901 cttgcaagct ttccgagcta attacactat tattttttct gggcttcgtc aggaacaagt
      961 agttgcatgg gtgggcttac tagtatgttt cattcttata gaagttaggc tgaaatccca
     1021 agaaaaacaa taaataaaag agctgactaa agtcagctct tttatttatt gaaagcctat
     1081 ccaagctcag gctcaatcag accatagctt ccattgcgcc tgcgatacag aacattaatc
     1141 ttgctcgtct ctgcattgta aaacacaaag aaattatcgt gccctaaatt acgcatttgc
     1201 actaacgctt cttcctcatt cataggatac ataacaaatt tcttgcgttt tgcaaaaagc
     1261 ggtgaaagtt gcccggtctc atcatcgatg atgtcgtcga cagcttcagc agccgaacgt
     1321 ccatcgccac gtccatgata cttcttccct ttatgccttt caatctgacg ctgcatatta
     1381 tccaatgctt tgtcaaatgc agccaaggct tcgtctgcgc gttcttctga gcgaagggta
     1441 aatccctttc caaacacagt aatctgcgca acatttcgat cattgacatc gcgtgcagct
     1501 tggtggtgcg aaagttccac gcgcgcatct tcaatagccg gcaagtaatg atcgaggttg
     1561 ccagccttct tcgaaacata ctcttcgatc cgttcggtca agcgaatatg acgtgtctga
     1621 acttccactt tattggacat aagatactcc ttttattagg ttgttaagtt acaaacacac
     1681 gctatacatg tctcaaatca tgatgagaca atgcacgcgc gactgtcaac gcatatactt
     1741 ccttcgcacc cgcagcataa agagcctctg cgccagagga aagagtcgaa ccagttgttg
     1801 aaacgtcatc cataataaga gctatttttc cttgcacccc tgatgcttga aaagcgtcat
     1861 gaacattctt ctgtctctcc tccctactca gccccacttg agaacgcgtc tcttttcggc
     1921 gcgccaaagc gtgaggtgaa tattgcaagc ccaaacccat agcaagtggt tttgcgatca
     1981 ttgcaacctg attatatccc ctttcatgca ttctttgttt tcctaacgga attggaatca
     2041 caagatcaat attccaattt aagctttgca caaacggcaa cattgctgcc gcaattgcat
     2101 cgcccataga aaagtcccgt cggtatttca acttatgcaa agctggctgg ataggctttt
     2161 cgaacacggt ccaagctctc aaagaatgaa aacgcggtct attcgcaaga caagtggaac
     2221 aaacaccagg cttatcttgc ggtagaccgc atacatcaca aacaatacca tccaacaatt
     2281 tcaggctact ttgacagata tcacaccatc ttgttccagg tttgccacag ccgccgcact
     2341 ctggcggaaa tagcaggtca agtccgctcc agatcgaacg atatgcttta tatttcaaat
     2401 tgtacatttg ctcccccctt tctgaaggtt aattttatag aaaccttaca tggctatact
     2461 aatttccaat gaaaggcccg aatgccccag caatctggaa accagcagga gccatcagaa
     2521 taatcataat tgaaggaaag gtcaaaagcg ccattgggat aatcattttt acaggagcct
     2581 tatgcgcctc ttcttcagca cgctggcgac gttttaaccg catctgatcc gattgaatcc
     2641 ttaaaacctt cgccatacta acaccaagga gttggctttg aatcactgca gccacaaaac
     2701 ttgtcaactc tggaagtcca atacgatccg ccatatcgcg caaggcttca cgttgtgttt
     2761 tcccaagttg aatctcacga atacatcgcc caaacataag ggaaagttca ttttcccatt
     2821 tgtccgcaac tttagacatt gctgcctcaa aacctaaacc ggcttccaca catatggtca
     2881 aaagatcaag tgcatctgga agtgccttac gcacttcatt ctgtcttcga gatatgcggc
     2941 ttccgagcca caactgtgga aagaagaagc ccaatagtgt aaagatagaa acgactaaga
     3001 aaacacgtcc aaatggccaa ttaacagttg gaaggtttga aattaataaa agcaacccgc
     3061 caaacacagc agcgcctaca aaacgagtag caagaaaagt agaagcatca atacgccctg
     3121 gattaccagc tagttcaagc ttcaaagctg tttcttggag aagtttatct ggggtgaatc
     3181 gagtggaaag ctctcccaat cgttttgcga ctgggataac aacacgctcc ataaatggtt
     3241 gctgaagttc aatatcttca agagaaacat tttcaccacg ttgggttgct tctgccaaac
     3301 gtgacaaaac tggatcagac tcgtcacctt gggcttcacg agcataacgc gccccaataa
     3361 caacaacaaa aacaatacta ccaaacaaag caataccaac gacaattgct atggctattc
     3421 ccatattaca cctcaatatc cgcgatcttc atcataataa agtaactaga tataattaaa
     3481 ccaagaacta cgcaaataat gaccgccgta caaatgggtc caccctttgt aactgataat
     3541 agataatctg gattcaagaa ccataatatt atagttaaac caaatggtat taagga
//
LOCUS       DAOQJE010000286         3591 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_16414_length_3591_cov_6.465215, whole genome shotgun sequence.
ACCESSION   DAOQJE010000286 DAOQJE010000000
VERSION     DAOQJE010000286.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3591)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3591)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3591
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16414_length_3591_cov_6.465215"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..606
                     /locus_tag="PLT08_08625"
     CDS             <1..606
                     /locus_tag="PLT08_08625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HNE04568.1"
                     /translation="YKYFGPHAGILYGKRELLEKLVAYKVRPATNELPGKFETGTQNH
                     EGIAGILGAIEYFEWVGKEFGGEYTEELKQQGYSGRRLTLKQAMSAIHAYEFELSRVT
                     LSTLEAIPGIHIFGNTDPRRLDERIATFSFRIGDMNPQTIAEKLAAENIYVWNGNYYA
                     INVSERLGVEEKGGMVRVGPVHYNTVEEINKLGDVLKKIAG"
     gene            complement(653..3391)
                     /locus_tag="PLT08_08630"
     CDS             complement(653..3391)
                     /locus_tag="PLT08_08630"
                     /inference="COORDINATES: protein motif:HMM:NF024938.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetate--CoA ligase family
                     protein/GNAT family N-acetyltransferase"
                     /protein_id="HNE04569.1"
                     /translation="METLVTPGFDPAHDVLHYGRQPLDSIFAPKSVAVVGATETVGSV
                     GRTIVWNLMSSSFGGTIYPVNPKRPSVLGIKAYPDLKSIPEKLDLIVVVTPAPTVPDI
                     IKEAVDLGVPSAIIISAGFKETGPAGVELERQVMEHARRGNMRIIGPNCLGVMSPTTG
                     LNATFASTIARRGSVGFISQSGALCTAILDWSLTENVGFSSFVSIGSMLDVDWSDLIY
                     YLGDDPHTKSIVIYMETIGNARAFLSAAREVAMTKPIIVIKPGRTEGAAKAAASHTGS
                     LTGSDEVLEVAFRRSGVLRVNSIAELFYMAEVLGKQPRPKGPRLTIITNAGGPGVLAT
                     DALISNGGELTTISPETMEEFNKLLPAAWSHNNPVDILGDASPERYAKALEIAAKDEN
                     SDGILVILTPQAMTDPTETADVLKPYAQGLGKPVLATWMGGKEVAPGEAILNKVNIPT
                     FPYPDTAARVFDYMAHYSENLHSLYETPMSMDDGGDGIDRERAAKLIEDVRKSGRTIL
                     TEYESKQLLACYGIPTVDTRIAKSKEEAVKAADAIGYPVVLKLHSETITHKTDVGGVQ
                     LNLADAKDVKKAYESIEKSVTEKAGAEHFLGVTVQPMAKVDGYELILGSSIDPQFGPV
                     LLFGLGGQLVEVFKDRALGLPPLNTTLARHMMENTRIYTALKGVRGRNPVDLAALERL
                     LVRFSQLITENRWIQELDINPLMASPEKLIALDARVLVYGKDVTQAQLPKMAIRAYPM
                     KYVSSWTAKDKTNVTIRPILTEDEKLLVRFHADLSDRSVFLRYLQPMMFQERVVHERL
                     SRICHCDYDREIALVAEKKVDGESIIMGVVRLSKLHANSEGRLSILVADPYQGTGLGG
                     EMVRRMIDVAKQEHLETISAILTDDNQVMGQLFKKLGFTIEATAENPKLMNALLML"
BASE COUNT          867 a          897 c          938 g          889 t
ORIGIN      
        1 tataaatact tcggtccaca tgcagggatt ttgtacggca agcgcgaatt gcttgaaaaa
       61 ctcgttgcct acaaagtgcg cccagccacg aatgaattgc ccggcaaatt tgagaccggc
      121 acccaaaacc acgaaggcat tgcaggcatt ctcggtgcca ttgaatactt tgaatgggtt
      181 ggcaaagaat tcggtggtga atacactgaa gagttgaagc aacaaggcta tagcggacgt
      241 cgcctgactc tcaagcaagc catgagcgcc atccatgctt atgaatttga actcagccga
      301 gtgacgcttt caaccctcga agcgattccg ggaattcata ttttcggcaa taccgatccg
      361 cgccggttgg acgaacgtat cgcgaccttc tccttccgca tcggcgacat gaatccgcag
      421 accattgctg agaaactcgc cgccgaaaat atttatgtgt ggaatggcaa ctactacgcg
      481 attaacgtct ctgaacggtt gggagtggaa gaaaaaggcg gcatggtgcg tgtgggaccc
      541 gtccactaca atactgtgga agaaatcaac aaactaggcg atgtgttaaa gaaaattgca
      601 ggctaaacaa aaaactccca gtcgaatgac tgggagtttt tatttaaaga tattacagca
      661 tcagcagagc gttcatcaac ttggggtttt cagcagtcgc ttcaatggtg aagcccagct
      721 ttttgaagag ctgacccatc acctgattat cgtccgtcag aatggcgctg atcgtttcaa
      781 ggtgttcctg tttggcaaca tcgatcatac ggcgcaccat ttcgccgccc aagccggttc
      841 cttgataggg gtcggcaacg agaatgctca aacgaccttc gctattggca tgtagtttgc
      901 tcaaacgcac cacacccatg atgatgcttt cgccatccac tttcttttca gcgaccaaag
      961 caatctcgcg atcatagtcg cagtgacaaa tgcgagagag gcgttcatgc acaacacgtt
     1021 cctggaacat catcggctgg agataacgca ggaatacgct gcgatccgaa aggtcagcat
     1081 ggaagcgcac gagaagtttt tcatcttccg tcaaaatcgg gcggatggtg acattggtct
     1141 tgtctttcgc cgtccacgag ctaacgtatt tcatcgggta cgcacggatc gccatcttgg
     1201 gcaattgcgc ctgcgtcaca tctttgccat acacaagcac acgcgcatct aaagcgatga
     1261 gcttttcagg tgatgccatg agcgggttga tatccaattc ttggatccag cggttctcgg
     1321 tgatcaactg tgagaagcgg acgagcaagc gttccaaggc ggcaaggtct acggggttgc
     1381 gtccacgcac accttttaag gcagtataga tacgggtatt ctccatcatg tgacgtgcaa
     1441 gagttgtatt gagcggaggc aatcccaagg cgcggtcttt gaagacttcc accaattgac
     1501 cacccaaccc gaacaacaac acgggaccaa actgcgggtc aatgctgctg ccgagaatga
     1561 gttcgtaccc atcaaccttt gccatcggtt gaaccgtcac accgaggaaa tgttccgcgc
     1621 ctgctttttc ggtcacagat ttttcaatgc tttcgtaggc tttcttcaca tccttggcat
     1681 ctgcaaggtt tagttgaacg ccgcccacat cggtcttgtg ggtgatggtc tctgaatgaa
     1741 gtttgagcac caccgggtag ccaatggcat ctgcggcttt gacggcttct tctttgctct
     1801 tcgcgatgcg agtatccacc gtcgggatgc cgtaacaagc caaaagttgt tttgattcgt
     1861 attctgtcaa aatggtgcga ccagatttgc gaacatcctc aatgagtttt gcagcacgtt
     1921 cacggtcgat cccatcgccg ccatcatcca tagacatagg cgtttcatac aagctgtgca
     1981 ggttctcaga ataatgcgcc atgtaatcga agacgcgcgc cgccgtatcg ggataaggga
     2041 aggtggggat gttcactttg ttgagaatgg cttcacccgg tgccacttct ttgccgccca
     2101 tccaagtagc gaggacgggt tttcccaagc cttgtgcgta tggcttcaag acgtctgccg
     2161 tttcggtagg gtcggtcatg gcttgcgggg tgaggatgac taaaatgccg tcgctgtttt
     2221 cgtctttggc tgcgatctca agtgctttgg cgtaacgttc agggctggca tcgccaagga
     2281 tatcgaccgg gttgttatgg ctccacgcgg cgggaagcag tttgttgaat tcttccatcg
     2341 tctctggtga gatagtggta agttcaccgc cattcgaaat gagtgcatcg gtggcgagca
     2401 cgccgggtcc gccagcattg gtaatgatcg taaggcgtgg acctttcggg cgcggttgtt
     2461 tgccgagcac ttctgccatg tagaacaatt cggcaatgct attcacgcgc aagacaccac
     2521 tgcgacggaa agcaacttca agcacttcgt cactaccggt caatgaaccg gtgtgagaag
     2581 ccgctgcttt ggctgcacct tcggtgcgcc ccggtttgat gacgatgatg ggtttggtca
     2641 tggcaacttc acgcgccgca gaaaggaaag cacgtgcatt accaatcgtt tccatataaa
     2701 tgacgatgct cttggtgtgt gggtcatcgc cgagatagta aataaggtcg ctccagtcca
     2761 catcgagcat ggagccgatg gagacgaagg aactaaagcc cacgttttcg gtgaggctcc
     2821 aatccaaaat ggcggtgcag agcgcgccac tctggctgat gaagcccacg cttccacgtc
     2881 gtgcaatggt gctggcgaag gtcgcgttca agccagtagt tgggctcatt acaccgaggc
     2941 agttcggtcc gatgatgcgc atgtttccac ggcgggcatg ttccatcacc tggcgctcaa
     3001 gttccacccc tgccggaccc gtctctttga atccagccga aatgatgatg gcgcttggca
     3061 caccaagatc cacggcttct ttgatgatat cgggcacagt cggcgccggt gtgacgacaa
     3121 cgataaggtc taatttttca ggaatggatt tcaaatctgg gtaggctttg atgccaagga
     3181 cgcttgggcg cttcgggttg acggggtaaa tggtgccgcc aaaagagctg ctcattaagt
     3241 tccaaacaat ggtccgcccc acgctgccaa ccgtttcagt agcaccgaca acggcaacgc
     3301 tttttggggc aaagatagag tctaggggtt ggcgcccata atgtagaaca tcatgagcgg
     3361 ggtcgaatcc gggagtgaca agggtttcca taatttactt ctttctttaa agggtacgtt
     3421 gttacgatgt tttttcatcg taacaatcag ttttgaagac aggatgatac aaagacatat
     3481 ataccactag aagcgggtgc tcaatttcac gtttcttata gattaacttc cttattcttg
     3541 attttttccc aacaaaaaag cggatgtctt cctaaaaaga catccgcttt c
//
LOCUS       DAOQJE010000287         3591 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_16417_length_3591_cov_5.168835, whole genome shotgun sequence.
ACCESSION   DAOQJE010000287 DAOQJE010000000
VERSION     DAOQJE010000287.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3591)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3591)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3591
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16417_length_3591_cov_5.168835"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..167
                     /locus_tag="PLT08_08635"
     CDS             <1..167
                     /locus_tag="PLT08_08635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="cyclic pyranopterin monophosphate synthase MoaC"
                     /protein_id="HNE04570.1"
                     /translation="TGKTGVEMEALTAVSVAALTVYDMAKAAEKTMHIQNIRLLEKHG
                     GMSGDVINEP"
     gene            178..546
                     /locus_tag="PLT08_08640"
     CDS             178..546
                     /locus_tag="PLT08_08640"
                     /inference="COORDINATES: protein motif:HMM:NF019498.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HNE04571.1"
                     /translation="MNYTSINLKEKLSKFSEHWSPKIIAQLNDYHLKLAKVQGEFVWH
                     DHPDTDEVFIVVQGHLDILFRDGKVSLDEGEMFVVPKGVEHKPVAEKECHILLIEPAG
                     LVNTGSAKDSELTAPNDVWI"
     gene            548..796
                     /gene="moaD"
                     /locus_tag="PLT08_08645"
     CDS             548..796
                     /gene="moaD"
                     /locus_tag="PLT08_08645"
                     /inference="COORDINATES: protein motif:HMM:TIGR01682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin converting factor subunit 1"
                     /protein_id="HNE04572.1"
                     /translation="MNHIKILFFATIRDRAGTKSIELDIPADATILQLKEKLSADYPN
                     LKESLKSVLVTINREYVFDEAVVPTNAEIALFPPVSGG"
     gene            806..1273
                     /locus_tag="PLT08_08650"
     CDS             806..1273
                     /locus_tag="PLT08_08650"
                     /inference="COORDINATES: protein motif:HMM:NF014448.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor biosynthesis protein MoaE"
                     /protein_id="HNE04573.1"
                     /translation="MQPSNHPTIYSITESEIDLNDLLAKITLTSTGAAAIFTGMVRGV
                     TTRGDAHETEYLEYESYVPMAEAKMQQVADEIREKWPVVEGIAIVQRIGKLYPKTPTV
                     LIACTAAHRDTGVFDAARYGIDRLKEIVPVWKKEVGPNGEEWVEGDYVPKPGE"
     gene            1283..1837
                     /locus_tag="PLT08_08655"
     CDS             1283..1837
                     /locus_tag="PLT08_08655"
                     /inference="COORDINATES: protein motif:HMM:NF024698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNE04574.1"
                     /translation="MHTQRLILRNFELADLDAYVGYRNDPEVGKYQGWVIPYSKEKGE
                     EFIREVQDMHAPKQGEWMQIAIALKETNVLIGDIGCVLRQEDARQAIIGYRIASPHWH
                     KGYAFEAVQAWLDFLFEDLNLHRVIADCDTENTASYKLLEKLGFRREAHFIESYLENG
                     GYTNEYHYGILQREWRAKAAFRNC"
     gene            1868..2392
                     /locus_tag="PLT08_08660"
     CDS             1868..2392
                     /locus_tag="PLT08_08660"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNE04575.1"
                     /translation="MVTIRKAVLKDASAIATVHVAAWKEAYRGIVADEVLNNLSIQRR
                     TEQWTNSLSDETHPYHRAFVAEMNSQVVGFSNFGAPQLKDLGFDGELFAIYILKAAHE
                     IGIGRSLVQAVVNGLREIGSKSMMVWVLKDNPARGFYERLGGVYLYEKPIQIGADELM
                     EVAYGWRDLSIFPA"
     gene            2530..>3591
                     /locus_tag="PLT08_08665"
     CDS             2530..>3591
                     /locus_tag="PLT08_08665"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: protein motif:HMM:NF005589.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HNE04576.1"
                     /translation="MQERIVITGMGTVNPLGLNAQESWTNAINGVSGVAPITQFDSHE
                     AKLQVHIAAEVKNFKAENFMDAREARRRDRFEQFAIAASKEALSSSGLQVTEKNAGRI
                     GIIISSAIGGLYSLQEATLTNYKEGPRKVSPFMIPMLMANGGAGMAAIEFGIKGPCFS
                     VASACASGADGIGTALMMLRTGMIDAALAGASEATITSTGVAAFDRVGAMSRRNDNYS
                     MTPQPFDKNRDGLVMGEGAAVLVLERESDAKARGANILAELAGYGATADAFHVTAPHE
                     HGAGGAAAIRMALDSAKANIDEVGYINAHGTGTSLNDQAETRAVKAAFGDLAYKTPIS
                     STKSMTGHMMGATGALEVIF"
BASE COUNT          934 a          713 c          968 g          976 t
ORIGIN      
        1 tgaccggcaa aaccggcgtt gagatggaag ccctaacggc tgtttccgtc gctgcgttga
       61 ccgtctacga tatggcaaaa gctgccgaga aaaccatgca catccaaaat attcggttgt
      121 tggaaaaaca tggcggtatg agcggcgatg ttattaatga accgtgatgg gtgaaccatg
      181 aattacacct ctattaatct aaaagagaaa ctctcgaagt tcagcgaaca ttggtcgccg
      241 aagattattg ctcaacttaa tgactatcat ctgaagctag ccaaagttca aggtgaattt
      301 gtctggcatg accatcctga tacagacgaa gtctttattg tggttcaagg tcatcttgat
      361 attctgtttc gtgatggcaa agtttcactg gatgagggtg agatgtttgt tgtgcccaaa
      421 ggcgtggagc ataagcctgt tgctgaaaaa gaatgtcata ttttattgat tgaaccagct
      481 gggctggtaa atactggtag cgcaaaagac tcggagttaa ccgctccaaa tgatgtatgg
      541 atataaaatg aatcacataa aaatcttatt ttttgccact atccgtgacc gtgcagggac
      601 aaaatctatt gagttggata ttcctgcaga tgcaaccatc ttgcagttaa aggagaagtt
      661 gagcgcagat taccctaact tgaaagagtc gcttaagtct gttttggtca ctataaaccg
      721 cgaatatgtt tttgatgaag cggtcgtccc tacaaatgct gaaatcgccc ttttccctcc
      781 cgtatctgga ggataatatt caactatgca accttcaaac cacccaacca tttattccat
      841 taccgagtct gaaattgact tgaacgattt acttgccaag atcacgctta catccacagg
      901 agcagcggcg attttcacgg gcatggtacg tggcgtcaca acccgtggcg atgctcacga
      961 gacggagtat ctcgaatatg agtcttatgt ccctatggca gaagccaaga tgcagcaagt
     1021 agcagatgaa atccgcgaga aatggcctgt agtggaaggt attgccattg ttcagcgcat
     1081 tggcaagctc tacccgaaaa cgccgactgt tcttattgcc tgtactgctg cccatcgtga
     1141 tacaggcgtt tttgatgctg cccgttatgg tattgaccgc ctcaaggaaa ttgtgcctgt
     1201 ttggaagaaa gaagtcggac ccaatggaga ggaatgggtc gaaggcgatt acgttcccaa
     1261 acctggagaa taagttttgt ttttgcacac ccaacgatta atcctgcgga attttgagct
     1321 tgccgacctt gatgcctatg taggctatcg aaacgaccca gaagtaggca aatatcaagg
     1381 ttgggtcatt ccttattcaa aagagaaagg ggaggagttt attcgagaag tgcaagatat
     1441 gcatgctccc aagcaaggtg agtggatgca gatcgcgatt gctttgaaag agactaatgt
     1501 attaataggc gatatcggtt gcgtattgag gcaagaagat gcccggcagg caattattgg
     1561 ttatcgtatt gcttcccctc attggcataa aggatatgct tttgaagctg ttcaagcctg
     1621 gttggatttt ttgtttgaag accttaattt acatcgtgtc attgcagatt gtgacacaga
     1681 aaacacagcc tcatataaat tgcttgaaaa gctaggtttt cggcgcgaag ctcattttat
     1741 tgaaagctat ttagagaatg ggggctatac caatgaatat cattacggca tccttcaacg
     1801 tgaatggcgt gccaaggctg catttcgtaa ctgttgattt gttctgagcc ttaagaaggg
     1861 agcaaagatg gtcactattc gtaaggctgt tttgaaagat gcttctgcga ttgctactgt
     1921 ccatgtggcg gcgtggaagg aggcgtaccg tggcattgtg gcggatgagg tgttgaacaa
     1981 tctgtccatt cagcggcgga cggagcagtg gaccaattct ctttctgatg aaacacaccc
     2041 atatcaccgc gcatttgtgg cggagatgaa tagtcaggtc gtgggttttt caaattttgg
     2101 cgcacctcaa ttaaaagact tgggctttga tggcgagttg tttgcaattt atattttgaa
     2161 agcggctcac gaaataggaa taggaaggtc attggtgcag gctgttgtca atgggttgcg
     2221 ggaaataggt agtaaatcca tgatggtgtg ggtgttgaaa gataacccag cgcgtggatt
     2281 ttacgagcgt ttgggtggtg tatatctcta tgaaaagcct attcagatcg gtgcagacga
     2341 gctgatggaa gtggcatatg ggtggcgaga tttgagcata ttccccgcgt agacccatgt
     2401 tttagagggc tatcaggcaa gggcggggga tgaagcgttg ccttaatttt atcgatggag
     2461 ggcgtcacga ttctgagaat cctgtgttgt taatatcgtt atattcattt ttttatcgtc
     2521 ggaggcgaga tgcaggaacg tatcgtgatt acaggcatgg ggactgtaaa cccacttgga
     2581 ttgaatgcgc aggaatcatg gacgaatgcg attaatgggg tgtctggtgt ggcgccaatc
     2641 acacagttcg actcacatga agcgaagttg caagtacata ttgcggcaga agtgaaaaac
     2701 ttcaaagcag aaaattttat ggatgcgcgc gaagctcgac gccgcgaccg ctttgaacag
     2761 tttgccattg ccgcttcaaa agaagctctg tcgagttctg ggttgcaggt taccgagaaa
     2821 aatgctgggc gtattggcat tattatttct tctgcgatcg gtggtttgta ttcattgcag
     2881 gaagccactc tcacaaatta caaagaaggt ccgcgtaaag taagcccgtt catgattccc
     2941 atgttgatgg cgaatggtgg ggcaggcatg gcggcaattg aatttggaat taagggacca
     3001 tgtttttctg tggcttcggc ttgtgcttct ggtgcagacg gaattggtac tgcgctgatg
     3061 atgctccgca cgggcatgat tgatgccgcg ctggcaggag cttctgaagc gacgatcaca
     3121 tctacgggtg tggctgcatt tgaccgcgta ggcgctatgt ctcgccgaaa tgacaattat
     3181 tcaatgaccc cccaaccgtt tgataagaat cgggatggtt tggtgatggg cgaaggggcg
     3241 gctgttctcg tgcttgaacg tgagtcggat gcgaaagcac gcggtgcaaa tattctggct
     3301 gaattagccg ggtatggtgc cactgctgat gctttccatg tgacggctcc gcatgaacat
     3361 ggggcgggcg gtgcggcagc aattcgtatg gcattggact ctgccaaggc taacattgat
     3421 gaagttggtt acattaacgc tcatggcacc ggaacttcgt tgaacgacca agccgaaacc
     3481 cgcgctgtta aggcggcatt tggtgacttg gcgtataaaa cgccaatttc ctcaaccaag
     3541 tctatgacgg ggcatatgat gggtgctacg ggtgctttgg aagtgatttt c
//
LOCUS       DAOQJE010000288         3582 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_16483_length_3582_cov_3.892827, whole genome shotgun sequence.
ACCESSION   DAOQJE010000288 DAOQJE010000000
VERSION     DAOQJE010000288.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3582)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3582)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3582
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16483_length_3582_cov_3.892827"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..237
                     /locus_tag="PLT08_08670"
     CDS             <1..237
                     /locus_tag="PLT08_08670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE04577.1"
                     /translation="NQTLIIRTKHNFHKERLDTPESKLTVGQAFAKILSFEPKFQIVL
                     EKTSAPVPETAASDPSLVSQALEMLGGKIIPEQG"
     gene            261..332
                     /locus_tag="PLT08_08675"
     tRNA            261..332
                     /locus_tag="PLT08_08675"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:293..295,aa:Gln,seq:ttg)
     gene            590..1060
                     /locus_tag="PLT08_08680"
     CDS             590..1060
                     /locus_tag="PLT08_08680"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HNE04578.1"
                     /translation="MVHRIRVASIITNNDKILLVKHVHPKTGYAWWVPPGGGVEESDT
                     SIFDCAKREVFEETNLTVEVSRILYIREFFDKENQRLNMEFFVLADKYSGESGLENLR
                     SSNGLDELYIKDVKWLSREEIQNIVVFPEILKDQFWDEHAMNFPSIKYLGRQNG"
     gene            1349..1840
                     /locus_tag="PLT08_08685"
     CDS             1349..1840
                     /locus_tag="PLT08_08685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE04579.1"
                     /translation="MSQTSLDFSEQLIKGKIAEMIFEQMLRDSGHFTVLGFGYEKVLP
                     ELMHRQADIEAKTTMEIIRRAPDFAIINNDSHEVHLIEVKFRSHNSPADILKIAQQLH
                     ASWKPAYLFLATPHGFYFGKTAEIIKAEGHIYPLDHPQISQDLQMKYLGLLNQFIGQS
                     TDK"
     gene            2157..2813
                     /locus_tag="PLT08_08690"
     CDS             2157..2813
                     /locus_tag="PLT08_08690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE04580.1"
                     /translation="MNNKEIPTTVGATLLVIITITMSVFVWTYEKGRSWGAVTIQTPV
                     LHHEQKDSGVEAALTEPISEWKTYSDEEFGFQFKYPKNFNVKISGYYSIGDSSKEWVS
                     FSVKKWKGYVIKENTPGTFWEKVSYVNWKYFSDNLKEIPLGKCNDEAMTHVTGETVAD
                     VPKTCIISRQGDLLKVETDNRIIYFTKDIEIRWELNQDNKNLVDKITSTFLLKSAQKK
                     "
     gene            complement(2881..3033)
                     /locus_tag="PLT08_08695"
     CDS             complement(2881..3033)
                     /locus_tag="PLT08_08695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE04581.1"
                     /translation="MTRGQHIADVGSVGAGTANHMHLEVYKKVDGAWTRVDPYGDGTN
                     NILWQH"
     gene            complement(3030..>3582)
                     /locus_tag="PLT08_08700"
     CDS             complement(3030..>3582)
                     /locus_tag="PLT08_08700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE04582.1"
                     /translation="DGLTAYNAPVSSVMDHSMTTPYAEDDIVLAFNGERGDKSNGFNL
                     GCYKKSGGGDFGSGFNYVGTTGTGGRPYFCYDGHPGYDYPQASGVDILAPAAGKLCVA
                     TTNITQQSPENIWRSPTACGNIPDVVTERWSDTGGFNAFYIFHTEYINGTTDDYMTVF
                     LHSNNLENTIVCGQPSHHKDMSS"
BASE COUNT         1085 a          754 c          801 g          942 t
ORIGIN      
        1 aatcagacac tcatcatccg gaccaaacac aacttccaca aggagcgtct cgacactccg
       61 gaaagcaaat tgacggtcgg ccaagccttt gctaagattc tctcgttcga gccgaaattc
      121 cagatcgttc tcgaaaagac ctccgcaccc gtaccggaaa cggcagcatc ggatccttcc
      181 cttgtttccc aggcgctcga gatgctcggc ggaaaaatca tacccgaaca aggctaggaa
      241 atatcacata ttcagactct tggggagtag ccaagcggta aggcaggggc ctttgaagcc
      301 cccatgcgaa ggttcgatcc cttcctcccc agctgatgaa ataaagcaga tatccatttt
      361 gatatctgct tttattttat ttgttagtaa gaggaaggga tcgaacggga gggggtcggg
      421 gaatgagagt tccccgtttg gggtgaccga cgaagtcggg ctagagggcg gaagccttat
      481 agaacgcagt ccgcaggacg agtagtgaga ttccctttct ctttattttt atacctaaaa
      541 tagattagta aaaacttatg gtacaattta ttcacactaa gattgactta tggtacatag
      601 aattcgtgtc gcatcaatta ttaccaataa tgacaaaata ttattagtaa agcatgtcca
      661 tccaaaaaca ggttacgcct ggtgggttcc gccaggcggt ggcgttgaag agagcgatac
      721 ttccatattt gactgcgcaa aaagagaggt ctttgaagag accaacctta ctgttgaagt
      781 ttccaggata ttgtatatcc gcgagttttt cgataaggag aaccagagat taaatatgga
      841 attttttgtg ttggctgaca aatatagcgg tgaaagcggg ttagaaaatc taaggagcag
      901 caatggcctt gatgaactct acatcaaaga cgtgaagtgg ctatcaaggg aagagataca
      961 aaacatcgtt gttttccctg aaattcttaa ggaccaattc tgggatgaac acgcaatgaa
     1021 tttcccgtct ataaaatacc tagggagaca aaatgggtaa taaatagttt ggtctgcatt
     1081 ttgacttcat tccagagaaa cggcagataa aagcgcctta gaatggcgct tttatgctat
     1141 actttaagaa aattacatat ttaaaagcaa atatgctttt ttcagaaaaa aatttcagat
     1201 ttttaaaaac aatcatcaag tggatcggcg tcatagtagc tctcatcgat gccctgtatc
     1261 ttttgctaag caggaagaaa aaattcaaag aattctggca acgctcggat caaagttcaa
     1321 atgatggcta tcataataaa tcccaactat gagtcagaca agcctggatt tttccgaaca
     1381 gcttatcaaa ggcaagatcg ccgaaatgat tttcgagcaa atgctgagag actcggggca
     1441 ttttaccgtt ctgggatttg gctatgaaaa ggttctgccg gagctcatgc ataggcaggc
     1501 agatatcgaa gcgaagacaa ccatggaaat catcagacga gcccctgatt tcgcgatcat
     1561 caataatgat tcgcatgagg tccacttgat cgaagtcaaa tttcgctctc acaatagccc
     1621 ggctgacatt ctgaaaatag cacaacagct ccacgcttct tggaaaccag cctatctctt
     1681 tctagcgact ccgcatggtt tctactttgg aaaaacagcg gaaatcatca aggcggaagg
     1741 gcatatatac ccgctggatc acccccaaat ttctcaagat ttgcaaatga aatatctagg
     1801 attactgaat caatttatag gtcaatctac ggataaatag gttccaactt cgtcccatag
     1861 ggacagctaa gaaaaatcat catgcccctt gtgggtatgg tgattttctt ttatcctgcg
     1921 gaggtaatgg atcgaacgag gaaaggggtc gggaaaacac ctgtttgcct gtttgaggtg
     1981 accggctttg ccgggctaga gggcggaagc cctatagaac gcagtggagc gagcagttcg
     2041 atctcccttc ctccccagca tttgtaaaaa gtcaccattt ttaggtgatt ttttatttta
     2101 caaaatattc ttatgatata ctttatgaag catgtagtag taaactaata caaaaaatga
     2161 ataacaaaga aatcccaact actgttgggg ctaccctatt agttatcata acaattacga
     2221 tgagcgtatt tgtatggacg tatgaaaaag gacggagttg gggtgcagtc acgatacaga
     2281 ctcccgtttt gcaccatgaa cagaaggatt caggtgttga ggctgccctg actgagccaa
     2341 taagtgaatg gaaaacgtac tcggatgaag aattcggttt tcaatttaaa taccctaaaa
     2401 attttaatgt aaaaatttct ggttactact caataggtga ttcttcaaaa gagtgggtaa
     2461 gtttttctgt taaaaaatgg aaggggtacg ttataaagga aaatacacca ggaacttttt
     2521 gggaaaaagt ctcttatgta aattggaagt atttttctga taatctcaaa gagattcctc
     2581 ttggaaaatg caacgacgaa gctatgacgc atgttactgg agaaacagtg gctgatgtac
     2641 cgaaaacctg cattattagt aggcaaggag atctcctcaa ggttgagacc gataatcgta
     2701 taatctactt tacgaaagat attgagatcc ggtgggaatt gaatcaagat aataaaaacc
     2761 ttgtagataa aataacctca acttttttat taaagtctgc tcaaaaaaag taataaaaaa
     2821 aactccctat cgaatattcc cgatagggag tctgccttta acagacatcg ctaggaagaa
     2881 tcagtgctgc cagaggatgt tgttcgtccc atcgccgtag gggtcaaccc gagtccatgc
     2941 cccgtctacc ttcttgtaca cctcaaggtg catatggttt gcagtaccag cgcccacgct
     3001 ccctacatcg gcgatatgct gaccccgagt cacgatgaca tatccttgtg atgcgacggt
     3061 tgaccacaaa caatcgtgtt ctccaagttg ttactatgaa ggaaaacagt catgtaatca
     3121 tcggttgttc cgttgatgta ctccgtgtgg aagatataga aggcattgaa gccgccagta
     3181 tctgaccatc gttcggtcac gacatcgggg atgttgccac atgcagtcgg actccgccag
     3241 atattttccg gggattgttg tgtgatattt gtagtagcga cacatagctt gccagcggct
     3301 ggagcaagga tgtccacgcc agaagcctga ggatagtcat agcctgggtg cccatcgtaa
     3361 cagaaatagg gtcttccgcc tgttccagta gttccaacgt agttaaagcc gctcccaaaa
     3421 tccccgccgc cactcttctt gtagcaacca agattaaagc cattgctctt gtcacctcgt
     3481 tccccattga aggcaagaac tatgtcatcc tccgcataag gagtagtcat ggaatgatcc
     3541 atcaccgaac tcactggcgc gttgtatgcc gtaagaccat ca
//
LOCUS       DAOQJE010000289         3580 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_16491_length_3580_cov_4.671206, whole genome shotgun sequence.
ACCESSION   DAOQJE010000289 DAOQJE010000000
VERSION     DAOQJE010000289.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3580)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3580)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3580
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16491_length_3580_cov_4.671206"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            30..959
                     /locus_tag="PLT08_08705"
     CDS             30..959
                     /locus_tag="PLT08_08705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE04583.1"
                     /translation="MLAAAVGAAIVVAAIMEQFKSRTVYILIAGFLLSSATITHYFNG
                     LTWMRSAVGMRDFWWQVSWRIPNLKPGTSIVANYSHAAVEEDYSVWGPANFIYYPQSA
                     SPTELQPVIWAVVLNDTTAGQILAKETPTKIDRRSVITFAGYDNILIISQPTQDSCVQ
                     VIDGTSPYFSEEEQSDIQQIKNSSNMQNIILDDSHSSIPPNEVFGAEPARTWCYYYES
                     ASLALQRGEYATVLDLKNQAEKKGFEPNDPVEWMPFIQAAAILNDQESIKEMARHIKK
                     VHSLEVQACENLNLLDLQIDTKNLIQSLFCVPE"
     gene            970..2289
                     /locus_tag="PLT08_08710"
     CDS             970..2289
                     /locus_tag="PLT08_08710"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: protein motif:HMM:NF015536.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016805 - dipeptidase activity [Evidence
                     IEA];
                     GO_function: GO:0070004 - cysteine-type exopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C69 family dipeptidase"
                     /protein_id="HNE04584.1"
                     /translation="MCDTLIATRLATTNGIAVFGKNSDRPPNEGQYLTQVPAADHVPG
                     SMLKCTYIEIPQVAHTHAVFLSKPFWMWGAEMGVNEHGLVIGNEAIYSKVPANKEPAL
                     LGMDLLRLALERAVTPREALDVIVQLLQEFGQGGNCLYDEPLYYHNSFLIANSEDAWV
                     LETIDKQWAARQVKDVYSISNCLTIGKQYDLSSENLVHFATQKKVTKSQSDFHFAQNF
                     NDFLYTNFGKGLKRRETTFNTLDSQKGKVNIETMMSTLRHHKDEHFNPQTSITEQDVC
                     MHAGFGPVRITQSTASMVVYLDKQQPIIFATGTSAPCTSLFTPFWMGCASFLNEEPVP
                     THQADANSLYWSHEKLHRAVLENYQERMGSYLQERNELEQRFITGAIKLQNASAAERT
                     EYSRACYAEKRTAEADWLKRAEAIPARKSVLHSMAWNGYNRKAGFSG"
     gene            2382..2858
                     /locus_tag="PLT08_08715"
     CDS             2382..2858
                     /locus_tag="PLT08_08715"
                     /inference="COORDINATES: protein motif:HMM:NF025054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNE04585.1"
                     /translation="MNITLQPVTISNWESLSGLKVRDDQKGFVGSNLASIAEAQFGFE
                     FENNGHWDLHPFGIYDGNLPVGFLMYGYNFGHSTWQAYIFRLMIDEKYQSRGYGRSGV
                     QHIIDVIRADERIKAIGISYKANNDVARRLYSSLGFVETGDKIGNEYLAVLTLRSS"
     gene            2972..3439
                     /locus_tag="PLT08_08720"
     CDS             2972..3439
                     /locus_tag="PLT08_08720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE04586.1"
                     /translation="MIKIKKYKIAQTIILAIVISVIWSIIQSFTAMATPTAPVTLVNH
                     ALNQCAEHVILGDECYYCTPIEGWEILDFGQQCPSGYTKTDSFAWSCTVHTPPATPHS
                     ECGGVNITVALLTPASGGSKPSVVKSNTIVYVGLALIVLSSIVLLISIRKKGS"
BASE COUNT         1071 a          760 c          772 g          977 t
ORIGIN      
        1 atgcagattt tggaaatcgt tcacgctata tgctggcagc tgcggtggga gctgcgatcg
       61 ttgttgcggc gatcatggag cagtttaagt cgcgaactgt atatattttg attgcagggt
      121 tcctgctttc cagcgcaacc attacacact acttcaatgg gctaacatgg atgcgctctg
      181 cagtaggtat gagagatttt tggtggcaag tttcatggcg tatccccaac ttaaaacccg
      241 gaacatctat cgtagcgaat tattcacatg cagcagttga agaggattat tctgtatggg
      301 gacctgcaaa ttttatttat tatccacaaa gtgcgagccc aacagaatta caacccgtta
      361 tttgggcggt tgtcttaaat gacactacag cagggcagat tttggcaaaa gagactccta
      421 ctaaaattga ccggcgttca gtaattactt ttgcaggcta cgacaatatt ctcattattt
      481 cgcaacccac tcaagattct tgcgttcaag taatagatgg aacttcacct tacttttccg
      541 aagaagagca aagtgatatt caacaaatca agaatagttc aaacatgcaa aatattattc
      601 ttgatgactc ccattcttca atacctccta atgaagtttt tggtgcagaa cctgctcgca
      661 catggtgcta ttactatgaa agtgcgtctt tagctctaca gcgtggagaa tatgcaactg
      721 ttctagacct gaaaaatcag gcggaaaaga aaggttttga acccaatgac cccgtggaat
      781 ggatgccctt tattcaagct gcggcgatct taaatgatca agagtccatc aaagaaatgg
      841 cgcgccatat taagaaagtt catagccttg aagttcaagc atgtgaaaat ctcaacttac
      901 tagatttaca aattgataca aaaaacttga ttcaatcttt attttgcgtt ccagaatagg
      961 aatctgacta tgtgtgacac ccttattgct acaagacttg ccaccaccaa cggcattgcc
     1021 gtttttggaa agaactcaga ccgcccgcct aacgaggggc aatacttaac tcaagtgcct
     1081 gccgctgacc atgttccagg cagtatgctg aaatgcacgt atattgaaat tccacaagtg
     1141 gcacacaccc atgccgtttt cctttccaag ccgttttgga tgtggggcgc agagatgggt
     1201 gtgaacgaac atgggttggt cattggcaac gaggcaatct actcgaaagt gcctgccaac
     1261 aaagaacctg ccctgcttgg gatggacctc ttacgcttgg cattggaacg ggctgtcact
     1321 ccgcgcgagg cgttggatgt gatcgtgcag cttttgcaag agtttgggca aggcggcaat
     1381 tgcttatatg atgagccgct ttactatcac aacagttttt tgatcgccaa ttctgaggat
     1441 gcctgggtac tagaaacaat cgacaagcag tgggcagcac gccaagtaaa agatgtatat
     1501 tcgatttcga actgcctaac aattggcaag caatacgatc tctcttctga aaacctggtt
     1561 cactttgcaa cccaaaagaa agttaccaag tcgcaaagtg actttcattt tgcgcaaaac
     1621 tttaacgatt ttctctacac caattttggt aaaggattaa agcgccgcga aactaccttc
     1681 aatacgcttg attcgcaaaa aggcaaagtc aacatagaaa caatgatgtc taccctgcgg
     1741 catcataaag acgaacactt caacccacaa accagcatca ccgagcaaga tgtttgtatg
     1801 catgcggggt ttggtccggt gcgcattacg caaagcacag catctatggt ggtgtatttg
     1861 gataaacaac agcccatcat tttcgccaca ggcacctcgg caccctgcac cagtctattc
     1921 actccctttt ggatgggctg cgcctctttt cttaacgaag agcctgttcc tacccatcag
     1981 gcagatgcga actctttata ctggtcgcat gaaaaactac accgtgccgt acttgaaaat
     2041 tatcaagaac gtatgggatc ttacctccaa gagcgcaacg aattggaaca acgcttcata
     2101 acaggcgcta taaaactaca aaacgcctct gctgccgaac gcaccgaata tagccgcgcc
     2161 tgttacgccg aaaaacgcac cgccgaagca gactggctca aacgcgcaga agccatcccc
     2221 gctcgaaaat ccgttttaca ctccatggcg tggaatggtt acaatcgaaa agcagggttt
     2281 tctgggtaag gcgttattaa gaagattttt ctggctggat agatgagtca agcaattaga
     2341 caccatagat aacccattga gctgattctg aaagaagtgt tatgaatatt acactccaac
     2401 cggtaacaat aagcaactgg gaatctttga gtggattaaa agtacgtgat gatcagaaag
     2461 gttttgtggg ttcgaatcta gcgtcgattg ctgaagcaca gtttggcttt gaatttgaaa
     2521 ataacggtca ttgggatttg catccctttg gtatctatga tggtaatcta ccagttggtt
     2581 ttttgatgta tggctataat tttggacatt caacatggca agcttatatt tttcgtttga
     2641 tgatcgatga aaaatatcaa agccgtggtt atggacgatc tggggtacaa catataatcg
     2701 atgtaattcg tgccgacgag cgcataaagg caataggaat aagttataag gctaataatg
     2761 atgttgcacg tcggttatat tcaagcctgg gctttgttga aactggagat aagataggga
     2821 atgaatatct cgcggtgttg acattgagaa gtagttaata aatagttcaa tccatttcta
     2881 tctcactaac acgtctcagc cttttttctg gctggacgga tgagttaggt ccgcgcaacg
     2941 gggcaggtaa tatctaacag taagatcaaa catgattaaa ataaagaaat acaaaatagc
     3001 tcaaactatc atactggcta tagtgatatc agtaatttgg tctatcatac agtcctttac
     3061 agccatggca acacctactg ccccagtaac attagtaaat catgccctta atcaatgtgc
     3121 tgaacatgta atactaggcg acgaatgcta ttactgtaca ccaatagaag gctgggaaat
     3181 tttagatttt gggcaacaat gcccatctgg atatacaaag acagatagtt ttgcatggag
     3241 ttgtacagtt cacactccac cagcgacacc gcactccgaa tgtggtggtg ttaatattac
     3301 tgttgctctt ctcacacctg cttctggagg ctctaaacct tcagtggtga aatcaaacac
     3361 catagtgtac gtagggttag cattaatagt tttgtctagt attgtcctgc taatatcgat
     3421 tagaaaaaag ggctcttaaa acattgtagt aaaagacata cctaacaaag cgtatcaaca
     3481 agtatcagaa ccactaccgt taggtaatgg gtaagttcag tggacaaaag ttatttacct
     3541 caggatttat tgtcataaat caagctatgt attaaagtta
//
LOCUS       DAOQJE010000290         3572 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_16548_length_3572_cov_6.531419, whole genome shotgun sequence.
ACCESSION   DAOQJE010000290 DAOQJE010000000
VERSION     DAOQJE010000290.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3572)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3572)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3572
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16548_length_3572_cov_6.531419"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..442)
                     /locus_tag="PLT08_08725"
     CDS             complement(<1..442)
                     /locus_tag="PLT08_08725"
                     /inference="COORDINATES: protein motif:HMM:NF012457.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 1 glycosylhydrolase"
                     /protein_id="HNE04587.1"
                     /translation="MPQGTYHFPKGFLWGTATASHQVEGNNTNNNWWKWEQEGHTNGS
                     AAIAADWWGGRWREDFDRAAETGQNAHRLSIEWSRVQPTPDRWDEDAIERYRTMLRGL
                     RDRNMTALVTLHHFTDPLWVTERGAWETEEIVPLFEKYVRKMVDS"
     gene            complement(492..1217)
                     /gene="rlmB"
                     /locus_tag="PLT08_08730"
     CDS             complement(492..1217)
                     /gene="rlmB"
                     /locus_tag="PLT08_08730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HNE04588.1"
                     /translation="MKEFIYSRNAVHEALLAKRRDIFSIEIAEGAQEKGTLAEIIKIA
                     QQRKIKLFRSPRPKLDKVNQHHQGVVAEVSGYPYADVVDILDKPKGEPPFILILDSLQ
                     DPQNFGTLIRTAEAVGVHGIIIPLARSVEVTPSAVNASSGASEHMLIAKSNLSQAMDA
                     LRDADIWLVGLDQAGAEVEANSRHLKGALGLVVGSEGEGIHELTRKKCDIVLKLPMKG
                     KIESLNAAVAGSVALYLAYLNRK"
     gene            1302..2129
                     /locus_tag="PLT08_08735"
     CDS             1302..2129
                     /locus_tag="PLT08_08735"
                     /inference="COORDINATES: protein motif:HMM:NF023166.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3298 domain-containing protein"
                     /protein_id="HNE04589.1"
                     /translation="MKNKKSLLLVLLLLVSLACSLTGTPTPETSAPLESIAPTFTPIP
                     SFTPEAQTASAALSSGLTVQSSPVEEAGTEPPYTIKAQVPYLQGSDDARVQSFNAYLK
                     QIVQNEIDGFKTNTLAYASTPPMVNGSSLEIQYSVLGQRGDIWSIQFLVYFYADGAAH
                     PGHYSISVNYDLANSREVTLDELFLPGSDYLTTLADISKAELATRDIGFESFSTGADP
                     LPENYTRWNLSNEGFLVITFDEYQVAPYAAGLQTVTIPVSQLQTVVNPNGVLPLFGK"
     gene            2131..3345
                     /locus_tag="PLT08_08740"
     CDS             2131..3345
                     /locus_tag="PLT08_08740"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNE04590.1"
                     /translation="MKPTLDIPLLFATRIIRMFCYGFLSVILVLYLSETGLTEQQAGL
                     LFTFTLAGDAAISLYLTTHADGFGRKRTLIIGALLMLGAGVVFLMTNNIVVLMLAAII
                     GVISPSGNEIGPFLSVEQASLSQLLPDKMRTKVFAWYNLAGSFSTAIGALTGGWLAQL
                     LQASGWSALDSYRYILTGYAVGGLLIAILFLQVSPAIEVHSEPNETKKVLGLHKSRNV
                     VLKLSAFFALDAFAGGLIVQSMIAYWFHIKFGVDSGILGSIFFGANILAGISSLLAVK
                     IADKFGLINTMVFTHIPSNIFLILVPLMPSLPLAIGILLLRFSISQMDVPTRQSYTMA
                     VVAPDERSAASGVTAIARSVGASLSPALTGIFFAAPALFNVPFFLCGGLKIIYDLLLW
                     REFRTVKPPEES"
BASE COUNT          784 a          983 c          823 g          982 t
ORIGIN      
        1 aagaatccac catcttgcgc acatatttct cgaacaacgg cacgatctct tcggtttccc
       61 atgcgccacg ttccgtcacc cacagcggat cggtgaagtg atgtaacgtt accaaggcgg
      121 tcatattgcg atcacgcagc ccgcgcagca tggtgcgata tctttcgatg gcgtcttcat
      181 cccatctatc aggtgtgggc tgcacacggc tccactcaat ggacagacga tgtgcattct
      241 gacccgtctc ggctgcacgg tcgaaatctt cgcgccaacg tccgccccac caatcggcag
      301 cgattgccgc cgacccattg gtgtgacctt cctgctccca cttccaccaa ttgttatttg
      361 tgttattgcc ttctacctga tgcgaagcag tcgccgtccc ccataaaaac ccttttggaa
      421 aatgataagt gccttgaggc atgatgatct ccatagtatc gtaggggcga cttgcaagtc
      481 gcccctacac attattttct attcaaatac gccaaataca atgccaccga ccctgccaca
      541 gcagcattca aagattcaat tttgcccttc atcggcaact tcaacacaat gtcacacttc
      601 ttgcgcgtca gttcgtggat gccttcaccc tccgagccga cgaccaatcc caatgctcct
      661 ttgagatgac gggaattcgc ctccacctcc gccccggctt gatccaaccc taccagccaa
      721 atgtctgcat ctcgaagcgc atccattgcc tgcgaaagat tcgacttggc gatgagcata
      781 tgctcactcg cgcctgaaga agcattcacc gcagagggtg tcacctccac tgagcgcgcc
      841 agcggaatga taatgccatg cactcccacc gcttctgcag tgcggatgag ggtgccgaag
      901 ttctgcgggt cttgcaacga gtcaagaatg aggatgaaag gcggttcgcc tttgggctta
      961 tccagaatat cgaccacatc tgcataggga tagccgctca cctctgccac cacaccttga
     1021 tggtgctggt tgaccttgtc taacttgggg cgcggcgacc ggaatagctt gatctttctc
     1081 tgttgcgcaa ttttgatgat ctctgccagt gtgccttttt cttgtgcccc ttcggcaatt
     1141 tctattgaaa aaatgtccct gcgtttggca agcaaggctt catggacggc attgcgtgaa
     1201 tagatgaatt ctttcatatc aaagtgtatt ttacttgata acgatatgtc cctgcataaa
     1261 tccacttaag cgatagaatc caagcattcc aaggagttcc aatgaaaaac aaaaagtctc
     1321 ttttactggt attgcttttg cttgtttcac tcgcctgctc gctgaccgga acaccaaccc
     1381 cagaaacctc tgctccgtta gaaagtattg cacccacctt cacgcctatc ccttcgttta
     1441 cgccggaagc acaaacagct tcagcggcgc taagctctgg gctgacggtt caatcgtcgc
     1501 cggtggaaga ggcggggact gaaccgccct acaccatcaa agcccaagtc ccttacttgc
     1561 aaggctccga tgatgcgcgt gtccaaagct ttaatgcgta tctcaagcag atcgttcaaa
     1621 atgaaataga tggcttcaag accaacacgc ttgcgtatgc cagcacgccg cccatggtga
     1681 acggaagctc gcttgaaatt caatacagcg ttctcgggca acgcggcgat atttggagta
     1741 ttcagttcct tgtttatttc tatgccgatg gcgcagcgca ccctgggcat tacagcatct
     1801 cggtcaatta tgatctggca aacagccgtg aggtaacgtt ggatgaactt tttctgccgg
     1861 gttctgatta tctaacgacc cttgctgata tttcgaaagc cgaacttgcc acacgcgata
     1921 ttggcttcga aagtttttct actggcgcag acccattgcc tgaaaattac acacgctgga
     1981 atctttcgaa cgaagggttt cttgtgatca cctttgatga atatcaagtc gcgccctatg
     2041 cagcaggtct gcaaacggtg accattcctg tttctcaatt gcaaaccgtt gtcaacccaa
     2101 atggagtatt gccattgttt ggaaaataac atgaaaccca cacttgatat tccattgttg
     2161 tttgccacgc gtattatccg tatgttctgt tatgggttct tgtcggttat tctcgtgcta
     2221 tatctttctg aaaccgggct cacagaacaa caagctggtt tgctctttac ctttactctc
     2281 gctggggatg ccgccatctc gctgtatctc accacacatg ctgatggctt cgggcggaaa
     2341 cgcaccctca tcatcggcgc tttgttgatg ttaggagcag gtgttgtttt cttgatgacg
     2401 aataacattg tggtcttgat gcttgccgcc atcatcggtg tcattagccc tagcggaaat
     2461 gagattggtc ccttcctctc ggtggaacag gcaagcctaa gtcaactttt gccagataag
     2521 atgcgcacta aagtctttgc ttggtataac cttgctggtt cattctccac agccattggt
     2581 gcgcttacag gcgggtggct cgctcaactt ttgcaagctt cgggttggtc tgctttggat
     2641 tcgtatcggt atattcttac agggtatgcc gtgggtggct tgttgatcgc gattttgttt
     2701 ttacaagttt cccctgctat tgaagtgcat agtgaaccca atgaaaccaa aaaagttttg
     2761 ggcttgcata aatcacgaaa cgtggttctc aagctcagcg ctttctttgc cctcgatgca
     2821 tttgcaggcg ggcttattgt tcaaagcatg attgcgtatt ggttccacat caagttcggc
     2881 gtggattcag gtattcttgg cagcatcttc ttcggcgcga atattctggc gggtatttca
     2941 tctttgcttg cagtaaagat cgcggataaa ttcgggttga tcaataccat ggtctttacc
     3001 cacattcctt ctaatatttt cttgattctc gtgccgctga tgccttctct gcctctcgcc
     3061 atcggaatcc tcctcctgcg ctttagcatc tctcaaatgg atgtgcccac gcgtcaatcc
     3121 tacacgatgg ctgttgtggc tccagacgaa cgatccgccg cctcgggtgt gaccgccatt
     3181 gcgcgttcgg tgggcgcgtc tctttccccc gcattaaccg gaatcttctt cgccgccccg
     3241 gcgttgttca atgtcccgtt ctttttgtgc ggcgggctta agatcattta cgacctgctg
     3301 ctctggcgcg aatttcgcac cgtcaagccg ccggaggaat cgtaggggcg accctggcgg
     3361 tcgccctctt ggttttttat ttttttggac aggggcaagc cctgtcccta cacaatgaaa
     3421 tggagaaacc atgtccactg cccccaaatc tcaaaccctg catctcgccg aaatggtcaa
     3481 ctaccaggac ggctctgtcg tcagccgtca gatcaccaaa gcggacgcgg gcaacgtcac
     3541 cctctttgct tttgattctg aacagggact ca
//
LOCUS       DAOQJE010000291         3570 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_16559_length_3570_cov_4.440398, whole genome shotgun sequence.
ACCESSION   DAOQJE010000291 DAOQJE010000000
VERSION     DAOQJE010000291.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3570)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3570)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3570
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16559_length_3570_cov_4.440398"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(68..1342)
                     /locus_tag="PLT08_08745"
     CDS             complement(68..1342)
                     /locus_tag="PLT08_08745"
                     /inference="COORDINATES: protein motif:HMM:NF024241.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNE04591.1"
                     /translation="MLNNHPLIQSLRELKGNPRITVWTEIMFGIPYNLFMPFFSVYML
                     ALGVTDQQIGSIASMGLVAQVFTALISGAIVDKFGRRLTLFIGDLLCWSVPCFIWAFA
                     QDVRFFVIAALMNSLWRISHTAWTCLMVEDAETRHLVHIWTWIMIFAFSSAFFAPLGG
                     WFVERYGLISAMRGLLIFGFVMLTAKGILLYIYSHETGRGIQRREETRHRSIISLLAE
                     YRSVFGQILHSRAILSALTLLVIMNIYSTITNNFWGVLFSSKLGFSDSQISTYVALRS
                     IVMTLGFFLIGPRLTNLRRFRLPLGAGFGVFLLSQVLLVLMPPKAVLFLVLSVVLEGA
                     GAALVNPMTESLLAVSMETDERARLTAMVSMAMIILISPFGWIAGQLSALDRSYPFLL
                     NIGLFAIGLVIVWIIGKPGFIPFVKQTEQQEI"
     gene            complement(1514..>3570)
                     /locus_tag="PLT08_08750"
     CDS             complement(1514..>3570)
                     /locus_tag="PLT08_08750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycoside hydrolase family 3 C-terminal
                     domain-containing protein"
                     /protein_id="HNE04592.1"
                     /translation="ALAEECIALNVDVLLGPGVNMKRSPLNGRNFEYFSEDPFLAGEL
                     AANFINGVQSKGVGTSLKHYAANNQEFQRFSISAEIDERTLREIYLPAFEKAVKQAQP
                     WTVMCSYNKVNGTFASEHHQLLTKILKDEWGFEGLVVSDWGAVRNRVAALKAGLDWEM
                     PGPQDLRVKAVVEAVRSGELNEATLDESVRRILRVVFKSKETPKGNGSFDVDAHHELA
                     RKIATEGVVLLKNDGLLPLKDHQHIAVIGRSAESAHFQGGGSSHINPTKVAMPYKELQ
                     SRAENAELTYAEGYPTDNSFQQGMIDDAVGLAEAADVAILYIALPTFKESEGYDRKDL
                     DLTVQQIALIKAVSKVQPNTVVVLNNGAPVAMSEWIDGVPAVLEGWMMGQAGGAALAD
                     ILFGRVNPSGKLAETFPLKLTDTPSHINWPGGAGKVHYGEGIFIGYRYYDTKEIPVLF
                     PFGHGLSYTTFEYSNAKVSSKNFKDVDGLTVTVDVKNTGKLAGKETVQVYVHDQKSDL
                     MRPVKELKGFAKVDLQPGETKTVSIHLDFRAFAYYHPEHKQWITEDGDFDILIGSSSA
                     DIRSHLTTTLQSTLDLPCVLDIESTMREWMADRRGRQVLGSLYEQIESQVRRLFAGED
                     RYGNTKAEEQVAEQTVMGMDIMDMMVDMPVVSVLMFQQSSLTMPAEEMVAGLLRQVHS
                     MK"
BASE COUNT         1026 a          872 c          817 g          855 t
ORIGIN      
        1 gacaatagcc aaagcagttt ttgtaccgcc gatatcacct actaatacat tcatcattac
       61 ctcaaatcta aatttcttgc tgctctgtct gtttcacaaa gggaatgaac cctggttttc
      121 ctataatcca aacaattaca agccctatag caaacaagcc aatatttaat aaaaatggat
      181 aggaacggtc aagggcagag agttgacctg ctatccagcc aaagggcgaa atcaaaatga
      241 tcattgccat ggaaaccatg gcagtcagtc tagcgcgttc atctgtttcc atagaaaccg
      301 ccaacaaaga ttctgtcata gggttaacaa gcgcggcgcc agcgccttcc aaaaccacgc
      361 tcaacaccag gaataacaca gctttaggcg gcataagaac caataacacc tgactcaata
      421 aaaagacacc aaagcctgcg cccagcggca aacggaatcg gcgcaaattg gtgaggcgtg
      481 gaccgatcaa aaagaagccc aaagtcatta caatggagcg taaagcaaca taggtggaaa
      541 tttgcgagtc agaaaagcct aatttgctgg aaaacaacac accccaaaaa ttatttgtga
      601 tggttgagta gatattcata atgacgagca atgttaacgc agaaaggatg gcgcgtgaat
      661 gtaatatttg tccgaaaaca ctgcgatatt cagccaacaa actaatgata gaacggtgac
      721 gggtttcttc tctgcgttgt atgccacgcc ccgtttcatg cgaatatatg taaagcaaaa
      781 tacctttggc agttaacata acaaaaccga agatcaacaa tccgcgcatg gcagagatca
      841 agccatatcg ttcgacaaac cagccgccta aaggtgcaaa aaacgccgaa gaaaaagcaa
      901 agatcataat ccatgtccaa atatgaacga ggtgacgggt ttcagcatct tcgaccatta
      961 agcatgtcca tgcggtgtgt gagatgcgcc aaaggctatt catcaaagcc gcaatgacaa
     1021 agaatctcac atcttgtgca aatgcccaga taaagcatgg cacgctccag cataatagat
     1081 cgccaataaa aagcgtcagg cgtcgcccaa atttatcgac aatggcgcca cttatgagcg
     1141 cggtgaaaac ctgagctacc aaacccatac tggcaatgct accgatttgt tgatcggtga
     1201 cacccaaagc cagcatgtat acagaaaaga atggcataaa aaggttgtat ggaataccga
     1261 acatgatctc ggtccagacc gtaatacgtg ggtttccctt cagttcacga agggattgaa
     1321 taagaggatg attatttaac attgtagtcc tgcaatatta ccaaaagctt tctataggtc
     1381 aaaccagttg aacaggcttg aaaatagcgt caataatggc gctattcgca atgaacagcg
     1441 ccacgtcaag ttacatcaag cctttagaaa aaaaataggg atgaaggctt tgataacctt
     1501 catcccaaaa gagctatttc atactatgaa cctgtcgtaa aagcccagcg accatctctt
     1561 cggcgggcat agtcagtgag ctttgctgga acatcaagac actcactaca ggcatatcaa
     1621 ccatcatgtc catgatatcc atgcccatca cagtttgttc agcaacttgt tcttctgcct
     1681 ttgtattccc ataacggtct tcaccggcaa acaacctgcg gacttggctt tcgatttgtt
     1741 cgtagaggct accaagcacc tgcctaccac gtcgatctgc catccactct cgcattgtag
     1801 attcaatatc cagaacgcag ggcagatcta aggtcgattg aagcgtggtg gtgaggtggc
     1861 tacgaatatc agctgaagaa gagccaataa ggatgtcaaa gtcgccgtct tcggtaatcc
     1921 actgtttgtg ttcggggtga tagtaagcga aggcacggaa atccaaatgg atggaaacgg
     1981 tcttggtctc gcccggctgg aggtccactt tggcaaagcc cttcaattct ttgacggggc
     2041 gcatcagatc agacttttgg tcgtggacgt acacctgaac cgtttcttta cctgcgagtt
     2101 tgcccgtgtt cttaacatcg acagtcaccg taagcccatc cacatctttg aaatttttgg
     2161 aagatacttt cgcgttgcta tattcaaatg tggtgtagct caaaccgtgc ccaaatggga
     2221 agagtacagg tatttctttg gtgtcgtaat aacgataacc aatgaaaatg ccttcgccat
     2281 aatgaacctt gccagcgccg ccgggccaat tgatgtggga tggagtgtcg gtaagtttga
     2341 gcgggaaggt ttcagcaagt ttaccgctgg ggttcacacg cccaaagaga atgtctgcaa
     2401 gagccgcgcc acctgcctgc cccatcatcc aaccttcgag cacagccgga acaccatcaa
     2461 tccattcact catggcaaca ggcgcaccat tattcaacac cacaaccgta ttcggctgaa
     2521 ctttggaaac agctttgatc aatgcaatct gttgaaccgt gaggtctagg tcttttcggt
     2581 catagccttc ggactctttg aaagtgggca aagcgatgta aagtattgcc acatcagcag
     2641 cctctgcgag acccactgcg tcatcgatca taccttgctg gaaggagtta tcggtggggt
     2701 agccttcggc gtaggtcaac tcggcgtttt cggcgcgaga ttgaagttcc ttataaggca
     2761 tggcgacttt ggtcgggttg atgtgtgagc tgccaccgcc ttgaaaatgc gcggactctg
     2821 ctgagcgccc aatgactgcg atatgctgat gatctttcaa cggtaataga ccatcgttct
     2881 tcaacagcac cacaccttct gtggcgatct ttcgtgccag ttcatggtgg gcatccacat
     2941 caaaggagcc gttccctttg ggagtttctt tggatttaaa aacgacgcgt aaaatacggc
     3001 gaacggattc gtccaatgtg gcttcgttca actcaccaga gcgaaccgcc tcgacaacag
     3061 ctttgacacg caagtcctgc ggacccggca tttcccaatc caagcctgct ttgagagctg
     3121 cgacccggtt gcgcaccgcc ccccagtcag acacgaccaa accttcaaag ccccattcat
     3181 ctttgaggat tttggtaagc aattgatgat gttcagatgc aaatgtgcca ttgaccttgt
     3241 tatatgagca catcaccgtc cacggttgag cttgtttgac agctttctca aacgcgggta
     3301 gatagatctc acgcagggtg cgttcgtcaa tctcggcact gatgctgaag cgctggaatt
     3361 cttgattatt ggcggcgtaa tgtttgagcg aagttcctac gcctttagat tgcacgccat
     3421 tgatgaagtt agcagccaat tcgcctgcaa ggaaggggtc ttcggagaaa tattcaaagt
     3481 tacgcccatt gagcggagac cgcttcatat ttacaccggg accaagcaag acatccacat
     3541 tgagagcgat gcattcttca gcgagggcaa
//
LOCUS       DAOQJE010000292         3569 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_16565_length_3569_cov_4.849175, whole genome shotgun sequence.
ACCESSION   DAOQJE010000292 DAOQJE010000000
VERSION     DAOQJE010000292.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3569)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3569)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3569
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16565_length_3569_cov_4.849175"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(325..609)
                     /locus_tag="PLT08_08755"
     CDS             complement(325..609)
                     /locus_tag="PLT08_08755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="MoaD/ThiS family protein"
                     /protein_id="HNE04593.1"
                     /translation="MLPYHLQNLARTGREVQIAVDGAPTIGSVLEALEKQYPMLRGTI
                     RDQATGQRRPFIRFFVCGADWSLEAWDVELPLAIQNGSEVFRVVGAMSGG"
     gene            complement(618..1730)
                     /locus_tag="PLT08_08760"
     CDS             complement(618..1730)
                     /locus_tag="PLT08_08760"
                     /inference="COORDINATES: protein motif:HMM:NF014110.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE04594.1"
                     /translation="MSGVRVLVGTRKGAFILTSDAKRKKWKVSGPHFAGWEIYHMHGS
                     SVDPNRLYASQSSGWFGQVIQRSNDGGETWEPVGNQFAYHGTPGTHMWFDGTQHPWEF
                     KRVWRLEPSLTDVDTVYAGVEDAALFRSTDGGQTWTELAGLRDVKGHLWQPGAGGMCL
                     HTIVLDPTNPKRMFIAISAAGAFRTEDGGETWSPINQGLSSGELPDADAEVGHCVHNI
                     SMHPTRPNVLFMQKHWDVMRSNDAGASWHEVSGNLPSDFGFPIAVHAHEPETIYVVPI
                     KSDSEHFPPDGKLRVYRSRTGGNDWEALTKGLPQENCYVNILRSAMAVDTLDSCGIYF
                     GTTGGQVYASSDSGDSWTPIVSNLPAVLSVEVQTLK"
     gene            complement(1781..2074)
                     /locus_tag="PLT08_08765"
     CDS             complement(1781..2074)
                     /locus_tag="PLT08_08765"
                     /inference="COORDINATES: protein motif:HMM:NF015925.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HNE04595.1"
                     /translation="MYAMTGTLTAQIGKRELLVNILVRASALVMTMPGCKAYIVLEDL
                     KDEQTVAVFEMWDDKEAHDVSLQDPRVRSLISEAMPILAGAPSGGEYRVATNL"
     gene            complement(2078..2440)
                     /locus_tag="PLT08_08770"
     CDS             complement(2078..2440)
                     /locus_tag="PLT08_08770"
                     /inference="COORDINATES: protein motif:HMM:NF013098.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HNE04596.1"
                     /translation="MSKRNIVHVEIPAVNTAAAGEFYQELFGWKTTPMPEMNYVMWEA
                     GSGDEYGGFPEVSAENPAGQVLVYIASDDIDEDLEKVSQLGGKVLREKTEIPGMGWYG
                     VFQDPTGNVLALYTGMNS"
     gene            complement(2602..3192)
                     /locus_tag="PLT08_08775"
     CDS             complement(2602..3192)
                     /locus_tag="PLT08_08775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE04597.1"
                     /translation="MRKLLSVFLALAVLFSFSYQRALAQGTLELLEARNDAGGGVIFV
                     FHYTGDFTANDFKGGLVILGDETFPMNCNITDKAEGIVQCTSSRVVAGHNVRLNLAGQ
                     VLYAFVPARSGGSGNGTIQICYNVYDTFEVPGGNEWQAFTTHCQDVPASYLDTINEDN
                     PYGGTYQYVFLGSDPGGCSVLNSINEDAYYFDFSGC"
     gene            complement(3345..>3569)
                     /locus_tag="PLT08_08780"
     CDS             complement(3345..>3569)
                     /locus_tag="PLT08_08780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE04598.1"
                     /translation="YFSVNGVCYQVPHGVPEHIVQEVGFNSCTPPSTPDPNNQDDDSG
                     EENSGGGGACPAPQTLVCTVTPRGQFCSCQ"
BASE COUNT          942 a          951 c          801 g          875 t
ORIGIN      
        1 cgggaaggcg gaatatccat catctgcttg agatgatcgc tgacaggcac gagatgtttt
       61 tcgtcaacag caagaacagg ggtggtattg gaaacggaca tggtgaattc tccttaagaa
      121 aaatgccccg aggctcgggg catttggtgt acttagtaag attgaacttg ttgctgttca
      181 cctgacaacc accaatgccc cgggcaccaa cgattggtgc gggtagtatt ggtattggtg
      241 tcaacaacga attgcttgaa cattgcgcgc aagcatacca cttgcatata catgcgtcaa
      301 gcaggaattt aggtttgcag taacttagcc gccagacatg gcgcccacca cacggaacac
      361 ttcgctccca ttttgaatcg caagcgggag ctcaacatcc caagcctcca aggaccagtc
      421 ggcaccacaa acaaaaaagc ggatgaaagg tctacgctga cccgtggctt gatcgcgaat
      481 ggtgccgcgc agcatgggat attgtttctc caacgcctcc aacacggacc cgatcgtagg
      541 cgccccatcg acagctatct gcacctctct gcctgtgcgc gccagatttt gcagatgata
      601 aggaagcaca acccgtatca tttcaaggtt tgcacttcca cagacaacac ggctgggagg
      661 ttactgacaa tgggcgtcca actatcgcca gagtctgaag aggcgtagac ctgcccacca
      721 gttgtgccaa aataaattcc acaagagtcg agggtatcca ctgccatagc agagcgcaaa
      781 atgttgacgt aacagttttc ctgcggcaaa cctttggtca atgcctccca gtcattccca
      841 cccgtgcggc tgcgatatac acgcagtttt ccatccggcg ggaagtgttc cgaatcactt
      901 ttgatcggca ccacgtaaat ggtctctggc tcatgggcat ggactgcgat cgggaagcca
      961 aaatcgctgg gcaaatttcc gctcacttca tgccatgaag cgccagcatc attgctgcgc
     1021 atcacatccc aatgtttttg catgaacaag acattcgggc gtgttgggtg catggaaata
     1081 ttatgtacac agtgacccac ctccgcatcg gcatcgggca attcgccgga agacaatccc
     1141 tgattgatcg gcgaccacgt ctcgccgcca tcttcagtgc ggaaagcccc tgcggcagaa
     1201 atagcgataa acatacgctt tggattagtc gggtcaagca caatggtgtg caggcacatt
     1261 ccacctgcgc cgggttgcca gagatgacct ttcacgtctc gcagtccggc taactcggtc
     1321 cacgtctgcc cgccatcagt ggagcggaat aaagctgcat cttccacccc ggcgtagacg
     1381 gtgtccacgt cggtcaaaga aggttccaag cgccaaacac gcttaaattc ccagggatgt
     1441 tgtgtgccat cgaaccacat atgggtgccg ggcgtgccat gatacgcaaa ttgatttcca
     1501 accggctccc aggtttcgcc gccatcattt gaacgctgaa tgacctgccc aaaccaaccg
     1561 ctagactgcg aagcatacaa gcggttcggg tccacagaag agccgtgcat gtggtagatc
     1621 tcccagcctg caaaatgcgg accgctcacc ttccattttt tacgtttggc atctgaggtc
     1681 aaaataaatg cccctttacg ggttccaact aatactcgta ctccgctcat cacgctctcc
     1741 ttggtcaata aatagtaacg acttaagctt gctctttcag ttacaggttt gttgctacac
     1801 ggtattcgcc accactcggt gctccggcaa gaatgggcat tgcctcagag attaggcttc
     1861 ttacacgagg gtcttgtaaa gatacatcgt gagcctcttt atcgtcccac atttcaaaca
     1921 cggcaacggt ttgctcgtct tttaggtctt ccagcacaat ataggctttg cagcccggca
     1981 tggtcataac caatgccgaa gcacgcacaa ggatgttcac taaaagctca cgcttcccaa
     2041 tttgagccgt gagggttccg gtcatagcgt acataaatta agaattcatc ccagtatata
     2101 aagccagaac attcccagtt gggtcttgga acaccccata ccaacccatg cccggaatct
     2161 cggtcttttc gcgcagtact ttgccgccca attggctgac cttttccaaa tcttcgtcaa
     2221 tatcgtcact ggcaatgtag accagcacct gcccagcagg gttctcggca gaaacttcag
     2281 ggaagccacc atattcatct ccactgccgg cttcccacat cacatagttc atttcgggca
     2341 tgggcgtggt cttccagccg aaaagttctt gatagaactc gcctgccgcc gcagtattca
     2401 cagcaggaat ctcaacatgg acaatatttc gtttggacat gacattctcc ttaattcgat
     2461 atttattatc cattatagaa cattttttct aattttcaag ccctcctcta gaattgtttt
     2521 tcgcttcctt tcaacaccca aacctgccta caaaaaagta gccctccatt taggagggct
     2581 actttactta taagctaaaa attagcatcc actgaaatcg aagtaatacg catcttcgtt
     2641 aatgctgttc agcacactac aaccgccggg gtcactgccc aaaaatacat actgatatgt
     2701 gccaccataa gggttgtcct cattgatagt atctaaataa ctagctggca catcctggca
     2761 atgtgttgta aaggcttgcc attcattccc acctggcacc tcaaacgtat catacacgtt
     2821 ataacaaatt tgaatcgtac cattgccgct accaccactg cgggcgggga caaaagcata
     2881 aagaacttgc cctgcaaggt tgagacgcac attatgccca gcaacgacgc gtgaagaggt
     2941 gcattgtacg atgccttctg ctttatctgt aatgttgcag ttcatgggga atgtctcatc
     3001 cccaaggatc accaagccac ctttgaaatc gttggcagta aaatcacccg tgtaatggaa
     3061 gacaaagatc acaccaccgc ctgcgtcatt acgcgcttcg agcaattcta aagtaccttg
     3121 agccagagct cgctggtacg aaaatgaaaa taaaactgct aaggctaaaa agaccgataa
     3181 aagctttctc atttttactc ctttttctaa agtagttgct taaattggaa aaaatgggtc
     3241 gaaacctata ctttgtttaa aatttagata gattatatct aaaaagcttt gtttaaggca
     3301 tcacagcccc attgcggttt aatggggctg tgatcatcta ctttttattg gcaagaacag
     3361 aattgcccac gaggagtaac tgtacaaacc aaggtttgag gcgcagggca agccccaccg
     3421 ccaccgctat tttcctcacc tgagtcatca tcctgattat tggggtcagg cgtggagggc
     3481 ggtgtgcagg agttaaagcc gacttcttgc acgatatgtt caggaacacc gtgcggaact
     3541 tggtaacaaa caccgttcac agagaagta
//
LOCUS       DAOQJE010000293         3568 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_16571_length_3568_cov_6.224879, whole genome shotgun sequence.
ACCESSION   DAOQJE010000293 DAOQJE010000000
VERSION     DAOQJE010000293.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3568)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3568)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3568
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16571_length_3568_cov_6.224879"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(78..872)
                     /locus_tag="PLT08_08785"
     CDS             complement(78..872)
                     /locus_tag="PLT08_08785"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNE04599.1"
                     /translation="MSKLTDKEYLTQKQYKDSTNLDVRVAIHKRFSTNSYGWFNWVFD
                     SFSALPENATILEIGCGTGELWKECESRIPKNWALTLTDISDGMLDAAWRNLIRIHRG
                     IKFEKVDSQSIPYADKTFDAVIANYMLYHVADRKKAFAEIKRVLKDDGVLFSATAGAN
                     HLREMYEWVFRVSGGRHGQIALQFTLENGKEQLQEFFPRVELSRYPDSLQITDVDMLI
                     AYVRSMASVELSEEEIQKLEQEWSEILVKDGAIHISKDAGLFQALQ"
     gene            complement(872..2893)
                     /locus_tag="PLT08_08790"
     CDS             complement(872..2893)
                     /locus_tag="PLT08_08790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE04600.1"
                     /translation="MKKKNFPEILSLLLISAIVYLPHIGSLTYYKDDWYYIYDGMLGG
                     AKIFHEMFRIDRPARGFFFEWYYTLFGPNPLPWHLSVFVWRALAALGALWIFNLLWKE
                     NRKFNFIAALLFAIYPGYFWWISAIEYQPMIASLALQVFSIAFTLKVVQSAKIAEKAT
                     YGIGSILTGWSYIALVDYAIGMEAFRFIAVYLLIAHDINDTFWKRLLAALKAWAWTVL
                     IPFGIVVWRIFFFTNERKATDIGGQLSVFFSNPVSVGIDWLLQIYNSVLNLSVQAWIN
                     QFPTFIQSMRLRDITLSIVLAVIVTLLVVFAEKQIKQSAETKDEGGKQAKVTNEALIL
                     GGLGMILGVLPVVMANRYVNISGFSHYGLPASLAAAVFMAGIIEIIALSSVRKAVLYG
                     VIVFASLSHFGIATNAAMEEKALQEFWWQVSWRVPDIQPGTTLVIYYPLPGMGDGGAA
                     LMEAANVIYFPEFTGDAIVHYPLSGLTLNSENLPGILDGTQIVESGYRSHTYTFDYTN
                     ILVMSQPTLNSCVHVMNASAPLISEYDPVDVSLVASQSKIENVIVDAEPVSPPEYIYG
                     KEPDTGWCSYFQAADLAAQTGDWERVAALGDEAIALSFAPEDRVEWLPFLKAYAIRGD
                     AETLKQLSKRVIGEKIIRLQACEMLSSIEQPLNDDVREMIDVNYCKGSE"
     gene            complement(2936..>3568)
                     /locus_tag="PLT08_08795"
     CDS             complement(2936..>3568)
                     /locus_tag="PLT08_08795"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025033.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNE04601.1"
                     /translation="SGPVQYEEYKTYSAGYEKRVCLDISIQALREARTRIGNHGLFVV
                     GDLANLPFKKDAFDGAVSMHAIHHLALPEHPKAYAEIHRVLAQGRTAAIVNGWHNPLL
                     IRMAEPLIGLMRKLSGRSEKKKKEWLSEEDPAGTFVQKMTPEWLKKEIGSKMTIEIKP
                     WRSMSTRIMRWFVRPFGGRAFLRFVFWLEGVFPKFFAENGQYPLIVVRKS"
BASE COUNT         1055 a          909 c          723 g          881 t
ORIGIN      
        1 ttgtttcggt aaaacaggct tacaacctgc taagacctgt tattctaaat tattaagcaa
       61 cttgcccatt aacaattcta ttgtagagcc tgaaatagcc cggcatcttt agaaatatga
      121 attgcgccat cttttactaa aatttcagac cactcttgtt caagtttttg tatttcttct
      181 tccgaaagtt ccacagaagc catcgagcgg acatatgcta tgagcatatc aacatccgta
      241 atctgcaaac tatctggata acgagataat tctacacgtg gaaagaactc ttgcaattgt
      301 tcttttccat tttcgagtgt gaattgcaat gcaatctgcc catgtcgccc gccactcaca
      361 cgaaaaaccc actcatacat ttctcgcaaa tggtttgcac ctgcagttgc agaaaatagt
      421 acgccatcat ctttcagaac acgtttaatt tcagcaaatg cttttttacg gtctgcgaca
      481 tgatagagca tgtaattggc aatcaccgca tcaaaggttt tatctgcata cggaatggat
      541 tgcgaatcca ctttttcaaa tttgatacca cgatggatgc ggatcaaatt acgccatgct
      601 gcatccaaca ttccatcaga aatatccgtt aacgtgagcg cccaattttt ggggatgcgg
      661 ctttcacact ctttccaaag ttcacctgtg ccacaaccta tttccaaaat ggtagcattc
      721 tcgggcaaag cgctaaaaga atcaaatacc caattaaacc atccatacga atttgtacta
      781 aatcgtttat ggatcgcgac ccgaacatcc aagttcgtag agtctttata ttgcttctgt
      841 gtcagatatt ccttgtccgt aagtttggac atcactcaga acctttgcaa tagtttacgt
      901 cgatcatttc acgcacatca tcgtttaatg gttgctcaat gctgctcaac atttcacacg
      961 cctgtaagcg gatgatcttc tcgccaatca cgcgtttgga taattgtttc aatgtttctg
     1021 catcaccacg aatggcatat gctttcaaaa aaggtaacca ttccacgcgg tcttcggggg
     1081 caaagctcaa agcgatagct tcatctccga gagcagcaac ccgctcccag tccccggttt
     1141 gagccgcaag gtccgctgct tggaaataac tacaccagcc agtatccggt tctttgccat
     1201 aaatatattc aggcggcgag accggttctg catccacaat tacattttcg attttcgatt
     1261 gtgaagcaac caatgaaaca tccactgggt cgtattcgga gatcaacggc gcacttgcat
     1321 tcatcacatg tacacatgaa ttcaacgtag gctgactcat taccaatata ttggtgtaat
     1381 caaaagtata agtatggcta cgatacccag actcaacgat ctgcgttcca tctaaaatac
     1441 cgggcaggtt ttcgctattc aaggtaagcc ccgaaagcgg ataatgcaca atagcatcac
     1501 ccgtgaactc agggaaataa atcacattcg cggcttccat caatgccgcg ccgccatcgc
     1561 ccatgccggg caacggataa tagatcacaa gtgtcgtacc aggttgaata tccggcacac
     1621 gccaactcac ctgccaccag aattcctgta gcgctttttc ttccatcgct gcattggtcg
     1681 caattccaaa atgagataac gacgcaaaca cgatcacccc atataacact gctttgcgca
     1741 cagatgacag cgcaataatc tcaataatgc cagccataaa aacagctgcc gccaaagatg
     1801 caggcaaacc ataatgtgaa aaaccggaaa tgttgacata acgattcgcc atcacaaccg
     1861 ggagaacgcc aagaatcatt cccagcccac caagaatcaa cgcctcattc gtgactttag
     1921 cttgtttgcc accttcatct ttggtctccg ctgattgctt gatctgcttc tcggcaaaga
     1981 ccacaagcaa agtcacgatc acagcaagca caatactcaa tgtgatatca cgcaaacgca
     2041 tcgactgaat aaaagtgggg aactgattaa tccatgcttg gacgctgaga ttcaaaacac
     2101 tgttatagat ttgaagcaac cagtcgatcc cgacggagac cgggttagaa aagaaaacac
     2161 ttaactgtcc gccaatatct gtggctttgc gctcattggt aaaaaagaag atacgccaaa
     2221 cgacaatgcc aaacgggata agaactgtcc acgcccaagc tttgagcgcg gcaagcaagc
     2281 gtttccaaaa tgtgtcgttg atgtcatgtg caatcaaaag atatacagca ataaagcgaa
     2341 aggcttccat gccaatggcg tagtcaacaa gagcaatgta actccagcca gtcaggatcg
     2401 aaccaatacc ataagtagct ttttcagcaa tcttcgcaga ctgtaccacc ttcaacgtga
     2461 acgcaatcga aaagacctgt aatgccaaac tcgcgatcat aggctggtat tcaatggcgc
     2521 tgatccacca gaagtagcca gggtaaatcg caaataaaag cgcggcaatg aagttaaatt
     2581 ttcgattttc tttccaaaga aggttgaata tccacaatgc gccaagggca gctaaagcac
     2641 gccaaacaaa gacactcaaa tgccacggca gcggattcgg tccaaagagg gtgtaatacc
     2701 actcaaagaa aaatccgcgc gccgggcggt cgatgcggaa catttcatgg aaaatcttcg
     2761 ccccacccaa catgccatcg tagatgtaat accagtcgtc tttgtaatag gtcaaactac
     2821 caatgtgcgg caaatacaca atggcagaaa taagcagaag tgagaggatt tcagggaagt
     2881 tttttttctt catgtacgat tgccgattta ctattttcga ctcacgtatt acgaattacg
     2941 atttacgaac cacgatcaac ggatactgtc cattttcggc gaagaacttc gggaagacac
     3001 cttccaacca gaaaacaaac ctcaagaaag cccgtccgcc gaaaggacgc acaaaccagc
     3061 gcataatgcg ggtgctcata ctgcgccacg gcttgatctc gatggtcatc tttgaaccga
     3121 tttctttctt cagccactct ggtgtcatct tctgaacgaa cgtcccagcg gggtcctctt
     3181 cgctcaacca ttccttcttt ttcttctcag agcgcccaga cagtttgcgc atcaaaccga
     3241 tgagcggttc tgccatgcga atcagcaacg gattgtgcca accattgacg atggcagccg
     3301 tgcgaccctg tgcaaggacg cggtgaattt ccgcataagc tttggggtgt tcgggcaagg
     3361 caagatgatg aatggcgtgc atagaaaccg cgccatcaaa agcatccttc ttgaagggca
     3421 gattcgccaa gtcaccaacg acaaatagac cgtggttccc aatacgagtc cgtgcttcgc
     3481 gcaaggcttg aatggaaata tccaagcaga cgcgcttttc gtagcccgca gaataggtct
     3541 tgtattcctc atactggaca ggaccaga
//
LOCUS       DAOQJE010000294         3561 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_16630_length_3561_cov_5.883628, whole genome shotgun sequence.
ACCESSION   DAOQJE010000294 DAOQJE010000000
VERSION     DAOQJE010000294.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3561)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3561)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3561
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16630_length_3561_cov_5.883628"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            207..2210
                     /locus_tag="PLT08_08800"
     CDS             207..2210
                     /locus_tag="PLT08_08800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE04602.1"
                     /translation="MSIKNHKKLTFGNYLLGLMALFLFVGCQASPTATSTALPPIKTA
                     SPAPTTTPTLAYQPESVSMENSGAWEFSRPLVFEDWNQWIISDNETYKLDDEYEIESV
                     DLSYQWWGMGGPSFEYQQIKRTGDVYKLNGRVVVKEKITNLVKSLSNLHAVPQTLNSI
                     THTDDYPIWAVEITGSDGTKILLTSDSNTSLYVPWNVIYNGDIYAQYDGSVEVALADL
                     FSVSEGEPMAAFYPGEGEEGKLIVSTGGWPKQLSYGFNGLLPVHTSFSYWTSPETGEI
                     IGIFDGRSSIGGFGNMVIGSIDNLERIVLEKDDGSKTNCDIETLESDDPSAKQWKFSC
                     ALTEPNAVGSYYYPIKVTFGTDKGERITSEGNLFGYWQRGINLSKTIFPLEISDSLKD
                     SEEFKKLAENHQIILYSFDAQVDQSTGNLSEGMNADVVLLGQINYKGKVLPYSVTTSM
                     GIKNGKIIRWDIDELELQNLISDILNQNITNNLLEAGQNPVLNLYYFEANTYVDIGYS
                     EGMTSSGRNWYQLGECKGYPWIEELPNETTALREFSVNSGWSKFDSQFILIDDEVRLY
                     DYRGTNLVNENNLLSFLLPKKIMISELPPLEISGQFRYGFYMFFVWGDNATQEEIETY
                     TNMINNLPGKKDFFDWGVKIDDVIFAVNEDGAFDMVECKSLQP"
     gene            complement(2301..3437)
                     /locus_tag="PLT08_08805"
     CDS             complement(2301..3437)
                     /locus_tag="PLT08_08805"
                     /inference="COORDINATES: protein motif:HMM:NF025019.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNE04603.1"
                     /translation="MKIQRLLLDELQKSLLRGKVLVLYGPRQAGKTTLAKDLMANTSL
                     RSKFVNADELLYREALSSQSLKTLNEVLGEAELLVIDEAQRVSEIGLNLKILIDNNPN
                     AIILATGSASFDLANKINEPLTGRKLTFTLYPLSYSELSKTLGSLETRNQLEQWLVWG
                     GYPVIATTSDLSLRERLLGELVGSYLYRDVLELGEIRRSEKIVDLLRLLAYQIGQEVS
                     IAELATSLGLSRDTVERYLDLLEKVFVIFRVGGFSRNLRKEVTKNSRYYFFDNGVRNS
                     LIQNLNPISVRNDAGQLWENFLMVERRKSNQFAERSVNAYFWRTYDQKEIDYIEEHAG
                     QLNAYEFKWGDGEIKSTTRKLFEEAYPDAKMQVINRENFEGFVM"
BASE COUNT         1057 a          734 c          735 g         1035 t
ORIGIN      
        1 gcggcgacaa cctcggtccc tcctggtctc ccgacggcaa ctggatcgtc ttcacctcct
       61 tcagagacgg caataacgaa ctctatatca tccaccccga tggcacggga ctgacgaggc
      121 tgacgaatag caccatctcc gattggcagc cgaggtgggg gaggtagcgg tataataaat
      181 gaacattcag acgaagggag ttaattatgt ctattaaaaa tcacaaaaaa ctaacatttg
      241 gaaattatct tttagggtta atggctttgt ttttatttgt tggatgtcaa gcgtcaccaa
      301 ctgcaacatc aaccgccttg cctccaataa aaactgcgtc tcctgcccca acaaccaccc
      361 ctacattggc atatcaaccc gaatctgttt ccatggagaa ttcaggtgca tgggaatttt
      421 ctcgtccact tgtttttgaa gattggaatc aatggattat ttctgataat gaaacttata
      481 aattagatga tgagtacgag attgagtctg tcgatctatc gtatcagtgg tgggggatgg
      541 gaggcccttc atttgaatat caacaaataa agagaacagg cgatgtttac aagctgaatg
      601 gaagagttgt tgtcaaagaa aagataacaa acttagtcaa atctctaagc aacttgcatg
      661 cagttcctca gactttaaat tcaattaccc ataccgatga ttatccgatt tgggcagttg
      721 aaatcactgg ttcagatgga acgaaaattc ttctaacttc agacagcaat acctcattat
      781 atgttccatg gaatgtgata tacaacggcg atatctatgc tcaatatgat gggagtgttg
      841 aagttgcatt agctgacctt ttctcggtta gcgaagggga gccaatggca gcattttatc
      901 caggtgaggg cgaagaagga aaattaatcg tcagtactgg aggttggccc aagcaactta
      961 gttatgggtt taatgggtta ttaccagtac atacttcctt tagttattgg acaagcccag
     1021 aaactgggga aatcataggt atatttgatg gtagaagttc tattggtggg tttggaaata
     1081 tggttattgg ttcaatagat aatcttgagc ggattgtttt ggaaaaagac gatgggagca
     1141 aaacgaattg tgatattgaa actcttgaga gcgatgaccc ttcagcgaag caatggaaat
     1201 tttcttgtgc actaacagaa ccaaatgccg ttggctctta ttattatcca atcaaagtaa
     1261 ctttcggaac agataaaggc gaaaggatta cttcagaagg taatctgttc ggttattggc
     1321 agcgtggcat caatctttct aagaccatat ttccgcttga aatatctgac tctcttaagg
     1381 actcagaaga atttaagaag ttggcagaaa accatcaaat aatcctttac agttttgatg
     1441 ctcaagttga tcaatcaaca ggtaatctaa gtgaaggaat gaatgcggat gtagttcttt
     1501 taggtcaaat aaactataag ggaaaagtat tgccatattc tgtcacaact tcaatgggta
     1561 ttaaaaatgg aaagattatt cgctgggata tagacgaatt agaattacag aacttaattt
     1621 cagatattct taaccaaaat attactaaca atttactaga agctggtcag aatcctgttt
     1681 tgaatcttta ttatttcgaa gctaatacat atgtcgatat tggatattct gaaggaatga
     1741 catcatctgg taggaattgg tatcaactgg gtgaatgcaa aggttaccct tggatagaag
     1801 agttgccaaa tgaaacgaca gcattgcgtg agttttcagt caatagcggc tggagcaagt
     1861 ttgattctca atttatcttg atagatgatg aagttagatt atatgactat agaggaacaa
     1921 acttagtaaa tgagaataat ttattgtcat tccttttgcc taaaaaaatc atgatctcag
     1981 aattgccacc tttagaaatt agcggtcaat tcagatatgg tttttatatg ttctttgtgt
     2041 ggggagataa tgctactcag gaagaaattg aaacctatac taatatgata aataaccttc
     2101 ccggaaaaaa agattttttc gattggggtg taaaaattga tgatgtcata tttgctgtaa
     2161 atgaagatgg tgcttttgat atggtcgaat gcaaaagttt gcagccataa tattccgtag
     2221 atttataaaa aacacctgca acttttcccc tccttgcctg tattacactc tagtcatgaa
     2281 tgcgcccatc gacagcgacc ctacatcaca aaaccttcaa agttttcgcg gttgataacc
     2341 tgcatcttcg catcgggata tgcttcttca aatagtttcc gcgtagttga cttgatctct
     2401 ccatcgcccc atttgaattc ataggcattc aactgtcccg catgttcctc gatgtaatca
     2461 atttccttct ggtcatacgt ccgccagaaa taagcattga ccgaacgctc tgcaaactgg
     2521 ttcgatttac ggcgttccac catcagaaaa ttctcccaca attgccctgc gtcgtttcgt
     2581 accgaaatgg gattcaagtt ttggatcagg ctgttgcgta caccgttatc gaaaaaatag
     2641 taacgtgagt tcttggtcac ctctttgcga aggttgcgag agaatccacc cacgcgaaaa
     2701 atgacaaata ctttttcgag caggtctaaa taacgttcca cagtgtcacg acttaaaccc
     2761 agacttgtgg caagttctgc aattgaaacc tcctgcccga tctggtaggc aagcaagcgc
     2821 agcaaatcca cgatcttttc tgagcgtcga atctcaccca gttccaacac atcgcgatat
     2881 aaataggaac caacaagttc tcccaacaaa cgctcgcgca gagacaggtc gctggtggta
     2941 gcgataaccg gataacctcc ccaaaccaac cattgttcca actggtttcg ggtttccaat
     3001 gagccaaggg tcttgcttaa ctccgaataa ctgagtggat aaagtgtgaa ggtcagctta
     3061 cgtcctgtta gcggttcatt gatcttattg gcgaggtcga aagatgccga accagtcgca
     3121 aggatgatag cattgggatt gttatcaatt agtatcttta ggttcaaccc gatttcagat
     3181 acacgctgcg cttcatcaat taccaacaat tctgcttcac ccaatacttc atttagtgtt
     3241 tttaaacttt ggctggacag cgcttctcga taaagtaact catcggcgtt gacaaatttt
     3301 gacctcaggg atgtgtttgc cattaaatcc ttggcaagag tagttttccc tgcctgtcgt
     3361 ggaccataca aaaccaaaac tttgccccga agcaggcttt tctgtaattc gtcgagtagt
     3421 aaccgctgaa ttttcataaa ttgattatac tacaccctct gaatttcgga tttccaaccg
     3481 ctctccctcc tgcaactttt ccccctcccc gcctgtatta cactctagcc atgaatgcgc
     3541 ccaccgaccg cgacctgatc c
//
LOCUS       DAOQJE010000295         3540 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_16773_length_3540_cov_6.134577, whole genome shotgun sequence.
ACCESSION   DAOQJE010000295 DAOQJE010000000
VERSION     DAOQJE010000295.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3540)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3540)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3540
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16773_length_3540_cov_6.134577"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..673)
                     /locus_tag="PLT08_08810"
     CDS             complement(<1..673)
                     /locus_tag="PLT08_08810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE04604.1"
                     /translation="MPTLEQLLRLPNIDSGLPFSISPDETRIVFSWNKTGSWELWEYH
                     DGALELVKIELEGAKFSPHFSPDGTKLAFALDPDGSESYHICVHDLITNTTTDLTPGI
                     LYANQPNMSWAPDGQSLAVLSEAKGIFSLYVLPLDDSGLHMLKNVFRPCWDAAWSPDG
                     MWIAIEAEAEASDRNIYIVPVGRRKGPRVGTTHLKVDGKALNAMHPTWSPDSKSLAFS
                     CENGEW"
     gene            complement(690..1178)
                     /locus_tag="PLT08_08815"
     CDS             complement(690..1178)
                     /locus_tag="PLT08_08815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE04605.1"
                     /translation="MLSKFIAFLKDLLLIPVVLGAIALFGQSLITTERVTCELQEDQF
                     YSCTAQDLIFGLRISERTAEKVHSIGYDLKCSESSDGRSCTAFASFKTEDGTSVQVSK
                     RYKDPDQVRKMTEMLNSLINEKSTSINITFPPSLFATILAGLFAVGSSFFIISNALEN
                     FK"
     gene            complement(1227..2705)
                     /locus_tag="PLT08_08820"
     CDS             complement(1227..2705)
                     /locus_tag="PLT08_08820"
                     /inference="COORDINATES: protein motif:HMM:NF018012.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0015948 - methanogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trimethylamine methyltransferase family protein"
                     /protein_id="HNE04606.1"
                     /translation="MRPKLTLLSDEIIARILAEAYELLLKPGIKVQNEEARELLRQEG
                     VVIDPETNVAQIPASLVQRALESVPREFYLYDYHGNPTIHYGGDSVHFDPGSSGITML
                     NPDTLEHDTTETEHLIRLLKVAEQIPQYDAQSTAVICHDVPKDIQDSYRLYLALLYSK
                     KPIVTGAFTNKTVQEMIDMLAMFAGSAEEARQKPRAIFDVCPAPPLIWSNFGAGNLIQ
                     LARAGIPAEIVSVPLAGAAAPVTMLGTVTQHTAECLAGITIHQLAKPGSPIVWGGAAA
                     IFDMKKGATPMGAIETAMLDCSYAQVAKTLGMPTHTYLGATDSKLVDAQAGLESGITA
                     MVGALSGINMISGSGMLDFLACHSAEKLVIDAEGIAMAKRMVMGVKQHTDTLATGFYD
                     DKINFKGGDFLKQKITMKLFREEQHLPSDIIDRNSTRQWKEAGSLDAFGRAKLRVKEL
                     LASYTRPELDPTQVARLHSYMLDLSKKAGVEELPKLEDYELA"
     gene            complement(2815..>3540)
                     /locus_tag="PLT08_08825"
     CDS             complement(2815..>3540)
                     /locus_tag="PLT08_08825"
                     /inference="COORDINATES: protein motif:HMM:NF018012.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0015948 - methanogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trimethylamine methyltransferase family protein"
                     /protein_id="HNE04607.1"
                     /translation="MTACMTTGPATMAGTLAVGNAEVIAATALLQLAYPGAPVFYAAA
                     QTASDPRTGVYTGGGPEDFLFGAATNVLADFYNIPLSMGSFATGAKEPNWQAGLEGSL
                     STFMASVAMSDMLLGCGFLHGSRIWSYAEMMMDCEIFSIIEKMMHGIEVNAETLALDA
                     VAAVGPGGHYLAQKHTRNHMREIFMPQFLDRRPYSKWEETGEDARDWAVNKARKVLKE
                     HQPDPLDEKISKEFERIIKSVEK"
BASE COUNT          847 a          808 c          923 g          962 t
ORIGIN      
        1 accattcgcc attttcacaa gaaaacgcca aagactttga gtctggtgac catgttggat
       61 gcatagcatt taaggctttg ccatctactt ttaggtgagt cgtcccaacc cgcggacctt
      121 tgcgtcgccc aaccggcacg atgtaaatgt tgcggtcgct ggcttcagct tcagcctcga
      181 ttgcgatcca catcccatct ggtgaccacg ctgcgtccca gcatgggcgg aaaacattct
      241 tgagcatatg caacccgctg tcgtcaagcg gcagtacgta taacgagaag atgcccttgg
      301 cttcggataa gacagctaga gattgcccat ctggcgccca agacatatta ggttggttgg
      361 catataaaat gcctggtgtc agatcggtag tggtgtttgt tatcagatcg tggacgcaga
      421 tgtgataaga ctcactccca tctgggtcta aagcaaaggc gagtttggta ccgtcaggcg
      481 aaaagtgagg ggagaatttt gcgccttcca gctcaatttt tactagttca agcgctccat
      541 cgtggtattc ccataactcc catgagccgg tcttattcca agaaaatacg attcgtgttt
      601 cgtctggcga aatgctaaat ggcagaccac tgtctatgtt ggggaggcgt agaagctgtt
      661 cgagagttgg cattaagaga gttttcttgt tatttgaaat tttcaagtgc attacttatg
      721 atgaagaagg atgaacctac cgcaaaaaga cctgctaaga tcgtggcgaa gagcgaaggt
      781 ggaaaggtta tattaatcga agtgcttttt tcatttatga gagagtttag catttctgtc
      841 attttgcgta cttggtctgg atctttgtat cgcttactta cttgaacgga agtgccatct
      901 tcggttttga atgaggcaaa ggcggtgcaa ctgcgcccat cgctactttc actgcatttg
      961 agatcgtaac caatactatg aactttttct gctgttcttt ctgaaattct taatccaaat
     1021 ataaggtctt gagcggtgca actatagaac tgatcttctt gtaattcaca agtaacccgt
     1081 tctgtggtta ttagagattg accaaacaaa gcaatagcac ctaaaactac gggaataagc
     1141 agaaggtctt taaggaatgc aataaattta cttaacatta aatactctct atactttgcc
     1201 ttttgtatat tattaatccg acacgtttat gccagctcgt aatcttccag ttttggtaat
     1261 tcttcaacgc ctgctttctt ggagaggtcg agcatgtatg agtgaaggcg agctacctga
     1321 gtagggtcca gctcagggcg ggtgtaagag gcgagcaatt cctttacacg cagtttggct
     1381 ctaccaaatg catctaacga accagcctct ttccactgac gagtcgagtt gcggtcgata
     1441 atgtcgctgg gcaagtgttg ttcctcgcgg aagagtttca ttgtaatttt ttgcttgagg
     1501 aagtcgccgc ctttgaagtt gattttgtca tcataaaagc cggtagcgag agtgtctgtg
     1561 tgttgtttga cgcccatcac catgcgtttt gccatagcaa tgccttctgc atcgatgacg
     1621 agtttttcgg cgctatggca ggcaaggaag tccagcatac cggagccgga gatcatattg
     1681 atgccgctga gtgcgccaac catagctgtg atgccgcttt caagaccggc ttgcgcatcg
     1741 acaagtttgg agtctgtggc accaagatag gtgtgagtgg gcatgccaag agtctttgca
     1801 acttgtgcat aggaacaatc aagcatggcg gtttcaatgg cacccatggg ggttgcgcct
     1861 ttcttcatgt cgaagatggc ggctgctcca ccccaaacga taggggagcc gggttttgcc
     1921 aattgatgga tggtgatgcc cgccaaacat tctgcggtgt gctgggtgac tgttccgagc
     1981 atggtgacgg gtgcagccgc accagccaac ggaaccgaga cgatctccgc tgggatgccc
     2041 gcacgtgcca gttgaattaa gttacctgcc ccaaagttag accagatcaa gggcggtgct
     2101 gggcaaacat cgaaaatggc gcgcggtttc tggcgtgctt cctctgcgct gcctgcgaac
     2161 atagcaagca tatcgatcat ctcttgcacg gtcttgttag tgaatgcgcc agtgacgata
     2221 ggtttcttgg aatagagcaa cgccaaataa aggcgatagg aatcttgaat atctttggga
     2281 acgtcgtgac agatcacggc ggtagattgc gcgtcgtatt gtggaatttg ttccgctact
     2341 ttcaataagc ggatgaggtg ttcggtctcg gtggtgtcgt gttcaagcgt atcggggttg
     2401 agcatggtga tgccagaaga gcccgggtca aagtggaccg agtcaccgcc gtagtgaata
     2461 gtggggttgc catgatagtc gtagagatag aactcgcgcg gcacgctctc caaggcacgt
     2521 tggacgagcg aagccggaat ttgcgctaca ttggtttcag ggtcaatgac aaccccttct
     2581 tgcctaagta gttcgcgagc ttcttcattt tgaactttga tgccgggttt gagcaaaagc
     2641 tcgtaggctt ctgccaaaat gcgggcaatg atctcgtcgc tgagcagggt tagctttggt
     2701 ctcatgagac acctctaatt agaatttctc tttcgtaaag agatataaaa cgagtaacga
     2761 gggcaagaaa tgagtcttct cacacctcgt tactcgtttc tcatttcttg tgcattactt
     2821 ttcgaccgac ttgataattc tttcgaactc tttgcttatt ttttcatcca acgggtcggg
     2881 ttggtgttct ttcaatactt ttcgagcctt gttaactgcc caatctcttg catcttcacc
     2941 ggtttcttcc catttggaat aagggcgacg gtctaagaat tgtggcatga agatctcacg
     3001 catatggttg cgagtgtgtt tttgggcgag gtaatgaccg cccggaccca ccgcagcaac
     3061 cgcatccaat gcgagggttt cagcattgac ttcaatgccg tgcatcatct tttcgatgat
     3121 ggagaaaatc tcacagtcca tcatcatttc ggcataagac catatgcgac tgccgtgtaa
     3181 gaaaccacat ccaagaagca tgtcagacat tgccacgctt gccataaatg ttgaaaggct
     3241 tccctcaagc ccagcttgcc aattcggctc ttttgcgccg gttgcgaagg agcccattga
     3301 aagcggaata ttgtagaaat ctgccaaaac attggtagcc gcaccgaaga gaaagtcttc
     3361 cggtccgccg ccggtgtaga cgcccgttcg cgggtctgaa gcagtttgag cggcggcgta
     3421 aaagacgggc gcgcccgggt atgccagttg taaaagggcg gtggcggcaa tgacttccgc
     3481 attgccgacg gcgagtgtgc cagccattgt ggcgggaccg gtcgtcatac aggctgtcat
//
LOCUS       DAOQJE010000296         3531 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_16843_length_3531_cov_3.149310, whole genome shotgun sequence.
ACCESSION   DAOQJE010000296 DAOQJE010000000
VERSION     DAOQJE010000296.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3531)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3531)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3531
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16843_length_3531_cov_3.149310"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(50..970)
                     /locus_tag="PLT08_08830"
     CDS             complement(50..970)
                     /locus_tag="PLT08_08830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE04608.1"
                     /translation="MKKLLSLFFALSLTLSACGTTSTQSTPPEQTETQPPFTYTSIYP
                     LTATLLPNTPPPPTLNLPPLLTPRVTPLPTRLPADIREGLSYMLQTNGDCVFPCFWGI
                     RPYQTRYEELYSVIDNLGGSRFETSQPEGAIHVSSYFNIEENHGIFVEFGANLQDDIV
                     ESMKIRLLNLYEDGVAVEDWSAYNLDKIQAIYGSPDKVELAFGQISNAIWFIVTLRYE
                     SISTVIIYDAAIPESKGNQFSTSDSVLFCPKEIVFGTVTLYMGNYPFNTIPSGLPILK
                     STGLTEQEFYKLFTENPSTCLTLNRKAFYP"
     gene            complement(1023..2420)
                     /locus_tag="PLT08_08835"
     CDS             complement(1023..2420)
                     /locus_tag="PLT08_08835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE04609.1"
                     /translation="MKKFLSLFFAISLTLSACGTASTQPAPPAQTGTPTVTPTTTQTS
                     TATITPLPTIPTITPTFDVSSIVTVTPANKEACLEENSSVVANFATPNNDGNYELYTP
                     SEILEYLNAGGSLSHLRDSSLGDIVDVTGDGLNEIIYRYGYWEYSILGCKNNIYQSLL
                     NFRGDFGVDLAEVSDLNKNGILEIILYDVVHYGYVNIFIFEWNGSEFNSLIDIGKEYP
                     TGPTIDRIRTTNYREFLDTNNDELEEIIATYDANQLCGGFGDFCDGTPIRKKTTTLKW
                     NGKNYVVDDMNYSLAQYRFQAIQDGDWQTGYGNYLEALTYYKAAIFDNKLDWWSLERE
                     KHERYTYMSQYDPTPVASPLPNPDPAEYPSLAAYAYYRIILLHLVQGQEAEAASAYQT
                     LQDTFGTDQYAAPYIEMTTAFWEAYQSTHKMYDGCAAAIQYAVEHPQILTPLGSDYHG
                     WQSHIYVPADVCPFR"
     gene            complement(2579..3286)
                     /locus_tag="PLT08_08840"
     CDS             complement(2579..3286)
                     /locus_tag="PLT08_08840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE04610.1"
                     /translation="MKKLFSLFFAISLTLSACGTASTQPELPAQTETPTVTPTVTQTP
                     TSIITPLPTIPTFTPTFDVSTIVTVTPSEKAACPKVNYAIPFEDGFKKIEACFKTAKK
                     DGSYPVACLNNNLHNEILDYLNSGGDIKKILTTLDGYNFGENNLYVYKDLTGDQIPEF
                     LLRDTFRFSYSGGYFFYKCSSGYYAILTDADQIPGITIQSIRDENFNGIPEIVFLIQN
                     TPIIIEWNGNAFVRIDN"
BASE COUNT          965 a          698 c          816 g         1052 t
ORIGIN      
        1 ggtcaactag tgttaggtgt ttgtgccgtt ggatccccat gaccaaaact tatgggtaaa
       61 acgcttttcg gtttagcgta aggcaagtgg atggattttc ggtaaacaac ttgtaaaatt
      121 cttgctcggt taatccagta ctttttaata ttggtaagcc actaggaatt gtgttaaatg
      181 gataatttcc catatacaaa gtaactgtac caaaaacgat ttctttaggg caaaaaagca
      241 cactatcaga tgtcgagaat tgattacctt tgctttctgg tatggctgca tcataaataa
      301 tcacagtact tatactctca tatcttaatg taactataaa ccaaattgca ttagaaattt
      361 gaccaaaggc tagttctact ttatcaggag acccataaat agcttggatt ttgtctaaat
      421 tataggcaga ccaatcctcc acagcaactc catcttcata caaatttaac aatcgtatct
      481 tcatgctttc aactatgtca tcttgcagat ttgctccaaa ttctacaaat atcccatggt
      541 tttcttcaat attaaaatag gacgaaacat gaatagcccc ttctggttgg gaggtttcaa
      601 aacgagaacc acctaaatta tcaataacac tatacagttc ctcgtaacgt gtttggtagg
      661 ggcgaattcc ccaaaaacac ggaaagacac aatccccatt tgtttgaagc atataactta
      721 aaccttctct tatatcagca ggcagtcttg tagggagagg agtcacacgc ggggttaata
      781 gtggaggaag attgagagta ggtggtggag gtgtattagg caacagagtc gcagttaaag
      841 gatatattga tgtataagta aaaggcggtt gtgtttctgt ttgctcaggt ggcgtggact
      901 gtgtggaagt tgtcccacaa gcggagaggg ttaggctgag tgcaaagaaa agggataaaa
      961 gttttttcat agtcctgcca ataaggtgac agtcatctcg cagatgactg tcacctgagg
     1021 tattacctga acggacagac atccgcaggc acgtagatgt gactctgcca accatgataa
     1081 tcactgccga gcggagtcag aatctgcggg tgctccacgg cgtattggat cgctgccgca
     1141 cagccatcgt acatcttgtg cgtggactga tacgcctccc aaaaggcagt tgtcatttct
     1201 atgtagggag cggcatattg atctgtgcca aaagtatctt gtaaagtttg gtacgcggac
     1261 gcggcttctg cttcctgtcc ctgcacgagg tggaggagga tgatgcggta ataggcgtag
     1321 gcggcgaggc tggggtattc ggcggggtca gggttgggga gtggagaagc gacaggtgtt
     1381 ggatcgtatt ggctcatata ggtataacgt tcgtgctttt ctcgttctag tgaccaccaa
     1441 tctaatttgt tatcaaaaat cgcagcctta taataagtca aagcttctaa atagtttcca
     1501 tagcccgttt gccagtcgcc atcctgaatt gcttgaaacc tatattgagc tagagaatag
     1561 ttcatatcat cgactacata attttttccg ttccatttca atgtagtagt tttttttcgt
     1621 atgggtgttc cgtcacagaa atctccaaac ccaccgcaga gttggtttgc gtcatatgtt
     1681 gcaataattt cttctagctc atcattgttt gtgtctaaaa attctctata attggtagta
     1741 cgaatccgat ctattgttgg tccagttggg tattctttcc ctatatctat tagcgagtta
     1801 aactcacttc cattccattc aaaaataaat atgttaacat aaccatagtg aacaacatcg
     1861 taaagaataa tttcgagtat tccattttta ttcaaatcag aaacctctgc tagatcaacc
     1921 ccaaaatcac ccctgaagtt taagagagac tggtaaatat tattcttgca acctaaaata
     1981 gaatattccc aatatccata cctgtaaatt atctcattca gaccatctcc agttacatct
     2041 acgatatctc ctaaactgga atctcgcaga tggctcaaac ttcctcctgc atttaagtat
     2101 tccaaaatct cggatggtgt ataaagttca taattgccat cattgttggg tgttgcaaaa
     2161 tttgcaacaa ctgaagagtt ttcttctaga catgcttctt tatttgcagg tgtgaccgtg
     2221 acaatggatg agacatcgaa ggtcggggtg atggttggga tggtgggcag aggtgtgatg
     2281 gtggctgtgg acgtttgagt tgtggtcggt gttacggttg gtgtccctgt ttgcgcggga
     2341 ggtgcgggct gcgtggatgc cgtcccacac gcggagaggg tcaggctgat tgcaaagaaa
     2401 agggagagaa actttttcat agttctgcca atcaggtgac agtcatctcg cagatgactg
     2461 tcaccttaat aattatctga atggacacat atccgcaggc acgtaggtgt gactctgcca
     2521 gccgtgataa tcgctgccga gcggagtcaa aatttgcgga tgctgttttg tagtctcgtt
     2581 aattatcaat tctaacaaag gcgttcccgt tccattcaat aatgataggc gtattttgaa
     2641 ttaggaatac aatttcagga attccgttga agttttcgtc gcgaattgat tggattgtta
     2701 ttcctggtat ttgatcagcg tcagtaagga tagcgtaata tccgctacta catttataga
     2761 aaaaataccc tcctgaataa gaaaacctaa aggtatcgcg caatagaaat tcaggaattt
     2821 ggtctccagt taagtctttg taaacgtata agttattttc gccaaaatta tacccatcaa
     2881 gagtagtaag aattttcttg atatcgccac ctgaatttag ataatcaagg atttcattat
     2941 gcaggttatt gtttaagcaa gcaacagggt atgaaccatc tttctttgct gttttaaaac
     3001 atgcttctat ttttttgaaa ccatcttcaa acgggatggc gtaatttact tttgggcacg
     3061 ctgccttctc cgatggtgtg accgtgacaa tcgtcgaaac atcgaaagtt ggtgtgaagg
     3121 ttgggatggt gggtaagggc gtaatgatgg atgtgggtgt ttgagtcacc gtcggtgtta
     3181 cggtcggtgt ctctgtttgt gcgggaagtt cgggctgcgt ggaggctgtc ccacacgcgg
     3241 agagagtcag gctgattgca aagaaaaggg agaaaagctt tttcatagtc ctccttgttg
     3301 atgggattat acttcgccat gtcatgctga gcagagcaaa gagtctctac gataatctca
     3361 gagatccttc gctttgctca ggatgacaga atcgagaatt atgacaaaca ctgctttcac
     3421 ctcgcatcgt tcgcccgtgt actcccgccg tggaattgtc gccacctcgc agccactggc
     3481 aactgctgcg ggcattgaag tcctttcgaa gggtggcaat gccgccgatg c
//
LOCUS       DAOQJE010000297         3522 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_16922_length_3522_cov_5.620998, whole genome shotgun sequence.
ACCESSION   DAOQJE010000297 DAOQJE010000000
VERSION     DAOQJE010000297.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3522)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3522)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3522
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16922_length_3522_cov_5.620998"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..794
                     /gene="trmD"
                     /locus_tag="PLT08_08845"
     CDS             <1..794
                     /gene="trmD"
                     /locus_tag="PLT08_08845"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: protein motif:HMM:TIGR00088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052906 - tRNA
                     (guanine(37)-N(1))-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HNE04611.1"
                     /translation="PKYYFLCYNRAMQFEVFTLLPEVFPSYLDTSILKRARERGLIHV
                     NVHNIRDYTHDRHNTTDDQPYGGGGGMVMKPEPVFEAIETVLGLASPPSTPEPESNIP
                     IILLTPQGRVFNQSIAKELSQHKKIALLCGRYEGIDERIRENLVTDQISIGDYVLTGG
                     ELPALILIDAVSRLLPNVLGDPTGAEDDSHAMGLLEYPHYTRPPEFRGNKVPDVLLSG
                     DHKKIDKWRREQALLRTLQKRPDMLETAELTKEDKKFLEKLKNQS"
     gene            851..2137
                     /locus_tag="PLT08_08850"
     CDS             851..2137
                     /locus_tag="PLT08_08850"
                     /inference="COORDINATES: protein motif:HMM:NF024742.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNE04612.1"
                     /translation="MMSSSRLNYGKTFLLGFGFFGVSVIWGVYNAFVPIFLSEKFNLS
                     PVLIGFFMTLDNIAALFIQPPVGAWSDKLRTPIGRRIPFILIGAPITALAFGLIPIAA
                     VLPLFVACTSTLLLSAALWRTPVVALMPDITPSEFRSQANGIINFMGGIGTIIALQTG
                     GILYKMSPSFPFWLGSGLVLIAALIVYLFIEEPKEYEAAEEQPGMFASLKEVVSDPDK
                     SGLRILLAIFFWFIGFSAVETFFTLYAKNHLGLNEGDGATLLSVLPLFFVLAAIPSGY
                     VAGKLGRKTTIATGLIIITIMLVLLYITPAEALLTGIAPLPLVGIPLVEGGARMLTLA
                     GVLLIIGGIGWAFININSLPMIVDLTSAARIGTFTGLYYLFSTLSAIVGPNLNGWAVQ
                     LTNGNYNIIMLIAPFFMLIALGLMMGVKKGEAITSN"
     gene            2160..3185
                     /locus_tag="PLT08_08855"
     CDS             2160..3185
                     /locus_tag="PLT08_08855"
                     /inference="COORDINATES: protein motif:HMM:NF014647.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HNE04613.1"
                     /translation="MFQTRNHLSRITKYFYLAMMAWALSACASSSDCFRKDVFCAALV
                     TDTLGIEDHGMNQNAWAGLQATQENGLADQVDYIASVDARDYQKNITYFVDLGYDVIF
                     TSGISLDDETLHSADLAPDSVFVGLNQTFEETHPNLISITFPEDQMGFAAGVGAASLT
                     KTGIVAAVCETSGIDAMWRYCEGFRAGVKHVSPELQVLVVYREDGDREKLFIDEQWGY
                     DTAKNLIERGADVVFAAGGVTGQGALKAATELHVYAIGSERDQAAVLGVQGSSVVTSV
                     YGQSQLTVQDVMRMLREGKLSKTWQGSVGYIPLSSAYPPEVSLGLDLLLGALKRGEIQ
                     TNVTKSH"
     gene            3294..>3522
                     /locus_tag="PLT08_08860"
     CDS             3294..>3522
                     /locus_tag="PLT08_08860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE04614.1"
                     /translation="MKKLYFVMAALIIASMVLAACGTPAATEAPAATEAAATEAAATE
                     APAATEAAAATIKVGEVTDLGGIDDKSFNANG"
BASE COUNT          841 a          908 c          852 g          921 t
ORIGIN      
        1 taccgaagta ctatttccta tgctacaatc gcgccatgca attcgaggtt tttacactcc
       61 tccctgaagt tttcccctct tatttagata ctagcatcct caaacgcgcc cgtgaacgcg
      121 gattgatcca tgtcaacgtt cacaacattc gtgactacac ccacgacaga cacaacacca
      181 ccgacgatca accctacggc ggcggcggcg gaatggtgat gaagccagag ccggttttcg
      241 aagccattga aaccgttctg ggacttgctt cgcctccttc cacgccggag cctgaatcaa
      301 acatcccaat cattttgctt acgccgcaag gacgcgtctt caaccaatcc attgccaaag
      361 aattatcaca acacaaaaag atcgccctgc tatgcggacg ttatgaaggc attgacgaac
      421 gcatccgcga aaacttggtg accgaccaaa tctccattgg cgactatgtg ctcactggtg
      481 gcgaactgcc cgcgctcatc ctaattgatg cggtttctcg tctcctgccc aatgtacttg
      541 gcgaccccac cggcgccgaa gatgactcgc acgctatggg cttgttggag tatccccatt
      601 acacccgtcc gcctgagttt cgcggcaaca aagtgcccga tgtgctcctc tctggtgatc
      661 acaaaaaaat tgacaaatgg cgcagagaac aagcactctt acgcacactt caaaagcgcc
      721 ccgatatgct tgaaactgca gagctcacaa aagaagataa aaagtttctt gagaagctaa
      781 agaatcaaag ttaaaacgtt ggcagactta agcatttaaa cctaccaacc tgtaaaccaa
      841 cctggaggct atgatgtcat cgtcaagatt gaactacggc aagaccttcc tgctcggctt
      901 cggttttttt ggtgtgagtg tgatctgggg tgtgtataac gccttcgttc ccatttttct
      961 ctctgaaaaa ttcaacctct cccctgtgtt aatcggtttc ttcatgaccc ttgataacat
     1021 tgcggcattg ttcattcagc cgcctgttgg agcatggtct gataaattgc gcacgcccat
     1081 tggtcgccgc attccattta ttttgatcgg cgcacccatt accgccttag cattcggctt
     1141 gattcctatt gcagcagtgc ttccgctatt tgtggcatgt accagcacgc tcttgctcag
     1201 tgctgcttta tggcgcaccc ctgtagtagc gctaatgcct gatatcacac cctctgaatt
     1261 tcgctcgcaa gccaatggca ttatcaactt catgggcgga attggcacca tcatcgccct
     1321 gcaaaccggt ggcatacttt ataaaatgag tcccagcttc ccattctggt taggctctgg
     1381 gctcgtgctc atagctgcgt tgatcgtata tcttttcatt gaagagccca aagaatacga
     1441 agccgccgaa gaacaacccg gtatgtttgc cagtctcaaa gaagtagtca gcgaccccga
     1501 taaaagcggt ttgcgcattt tgcttgccat ctttttctgg ttcattggtt tttcagcagt
     1561 agaaactttc ttcacacttt atgccaagaa tcatctcggc ttgaacgaag gtgacggcgc
     1621 aaccttactc tccgtcctgc cactgttctt tgtgctggct gccatccctt ctgggtatgt
     1681 ggctgggaaa cttggacgca aaaccaccat tgccaccggt ttgatcatta tcaccatcat
     1741 gttggtcttg ctatacatca cccctgctga agcgttgctc accggcattg cacctctgcc
     1801 actggtcggt attccgctcg tggaaggcgg cgcacgcatg ctcacactgg ctggcgtttt
     1861 gctcatcatc ggcggcatcg gttgggcatt catcaacatc aactctctgc ccatgatcgt
     1921 ggatttgacc agtgcggcgc gcatcggtac attcacaggt ctctattatc tcttctccac
     1981 cttatctgcc attgtgggtc ccaacctgaa tggttgggca gtacaactca cgaatggcaa
     2041 ttacaacatc atcatgttga tcgctccatt ctttatgttg atagcgctcg gtttgatgat
     2101 gggcgtcaaa aaaggtgagg caatcacaag caattaatga gtaccaagtg aagagcaatt
     2161 tgtttcaaac aagaaaccac ctttcacgaa tcacgaagta tttttatctg gcgatgatgg
     2221 catgggcact gagtgcttgt gcgtcatcgt cagattgttt tcgtaaagat gttttctgcg
     2281 ccgcattggt gacggacacg cttggcattg aagaccacgg catgaatcaa aatgcatggg
     2341 cagggttaca agcaacgcaa gaaaatggct tggcagatca agtggattac attgcctctg
     2401 tcgatgcgcg cgattatcaa aagaatatca cgtattttgt agaccttggc tacgacgtca
     2461 tctttacatc tggcatttca ttggatgacg aaacgcttca cagtgcggac ctcgcgcctg
     2521 attctgtttt cgtgggctta aaccaaacct ttgaagaaac ccaccccaac ctaatctcga
     2581 tcactttccc tgaagaccaa atgggctttg ccgctggggt tggcgccgca tcgttgacca
     2641 aaacgggcat cgttgcggca gtatgtgaaa cctctggcat tgatgccatg tggcgctatt
     2701 gcgaaggctt tcgggctggc gtgaaacacg tcagcccgga gttgcaggtt ttggtggtgt
     2761 accgtgaaga tggagataga gaaaaactgt tcattgatga acagtggggg tatgacacgg
     2821 cgaaaaatct aattgaacgc ggagccgatg tggtctttgc cgcgggcgga gtcaccgggc
     2881 agggggcgtt aaaagccgcc acagagttgc atgtgtacgc tatcgggtca gagcgcgatc
     2941 aggcggcggt tttaggggta caaggctcga gcgtggtcac ttcggtatat gggcagagtc
     3001 agttgacggt tcaggatgtg atgcgaatgc tccgtgaggg caaactctct aagacatggc
     3061 aaggctcggt ggggtatatc ccgctgagtt cggcgtatcc gcctgaggtt agtttggggt
     3121 tagacctctt gttgggggct ttaaagcgag gggaaatcca aacaaatgtc acaaaatctc
     3181 attaacattg cgcagtcttg cacaaagatt ggagtagatt tatacttgtt aggtcgtgag
     3241 gctgtacgaa aacagtcttt ccaatcacta tcttctagag gagaagacat aaaatgaaga
     3301 agctttattt tgttatggct gctttgatca tcgcctcgat ggtcttggct gcctgtggca
     3361 ctcctgctgc cactgaagcc cccgctgcta ctgaagctgc cgctaccgaa gctgctgcta
     3421 ccgaagcccc tgccgctacc gaagctgccg ctgccaccat caaagttggt gaagtgaccg
     3481 acctcggcgg tattgatgac aagtccttca atgccaatgg tt
//
LOCUS       DAOQJE010000298         3512 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_16996_length_3512_cov_6.746312, whole genome shotgun sequence.
ACCESSION   DAOQJE010000298 DAOQJE010000000
VERSION     DAOQJE010000298.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3512)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3512)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3512
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16996_length_3512_cov_6.746312"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2167
                     /locus_tag="PLT08_08865"
     CDS             <1..2167
                     /locus_tag="PLT08_08865"
                     /inference="COORDINATES: protein motif:HMM:NF019966.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HNE04615.1"
                     /translation="VPLAKPFKKPKKLEYAYLTTEGEDLDKKHFLTVDWKLPDASDPV
                     QAMSFRILTHALIGTPASPLYKALTDSGLGEDLAGLGLESDLREMIFSTGLKGTKARN
                     AKKIETLIFDTLQKLVNEGIDPDTIAASINTLEFAMRENNTGGFPRGIAIMLRALTTW
                     LYDDDAFKTLAFEAPLAEIKNRLAADSRYFEKMIQTHLLDNVHRTVIRLTPDVELAQR
                     LEAEETLRLDSARKTMSDDDIRKHIENTEKLKIRQETPDNPEDVAKLPVLDLEDLDKQ
                     ARTIPIEEIQVQDSKVLYHDIFTNGIVYLDLAFDMRAMPQELLPLTEVFARALFEVGT
                     ETEDYVKLSQRIGKSTGGIYGSSVTATLQGSRESRAYLTIRGKSTMSQANEMLSILRD
                     VLLTVKFDNKERLKQIVMEEKAGLESGLAPAGHRFANMRLRSQFGGTGYINDQTKGIG
                     YLFALRELATEIDKKWANVLKKLETIRETLINSKTLLCNVTLDAHNWKTFQPHLENFI
                     FAMPVKDVELSPFNFAPSTQKEGLAIPAQVNYVAKGANLYDHGYELDGSSSVIVGYLG
                     MTHLWEKIRVQGGAYGAFAQFDDATGVFTYLSYRDPNVDNTIASYDSAAAFLKNLDAS
                     RLNDNELKKSIISAIGNLDSYQLPDAKGYTSMMRFITGRSDEIRQKYRDQVLSTNGED
                     FIAFGEVLEKVAQSDAVVVLGSQSALEGSKAGLKVMKVA"
     gene            2207..2704
                     /locus_tag="PLT08_08870"
     CDS             2207..2704
                     /locus_tag="PLT08_08870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE04616.1"
                     /translation="MAGLEEQLVSELWARDVRFILGTAPNHPPMLSPVELIKGLAVSV
                     ETRLQLSLIPVFLRHPEFSIYAESVAKNLPLEKQIILKCFYCAAVWLECKHLSQNNLP
                     DLFSKDLGLLPTSDPDNNLLLLANRQKELSSSRINWAATYQHAADIWLKDLELRKMKN
                     GKRNS"
     gene            2685..3197
                     /locus_tag="PLT08_08875"
     CDS             2685..3197
                     /locus_tag="PLT08_08875"
                     /inference="COORDINATES: protein motif:HMM:NF039330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6036 family nucleotidyltransferase"
                     /protein_id="HNE04617.1"
                     /translation="MEKETLESILKEFGKRVPPGSQLHLLGGGALTLLGSPRPSLDID
                     FVGDDVHPNELHRTILSIAKEMDLFVDAVPIDRFVPLPEGHENRAIHIGQFENLEIFV
                     IDPYSIALSKVDRGSFTDYDDILFLIQKRYIDMSKLEEIIKEAVAKAGKYDLHPEILS
                     HFEELKSRLS"
     gene            3332..>3512
                     /locus_tag="PLT08_08880"
     CDS             3332..>3512
                     /locus_tag="PLT08_08880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE04618.1"
                     /translation="MNITLKINGIEHTIEAPASTTLLAALRGLGFHGVKFGDEQGLTG
                     ADTVLLDGKPINAGSM"
BASE COUNT          955 a          928 c          821 g          808 t
ORIGIN      
        1 ggtgccgctt gccaaaccgt tcaagaaacc gaaaaagctt gagtatgctt acctcaccac
       61 cgaaggcgaa gacctcgaca aaaaacattt cctcactgtc gactggaaat tacccgacgc
      121 ctccgacccc gtgcaggcga tgagtttccg catcctcaca cacgccctca tcggcacacc
      181 cgcttcccca ctttataaag cgctcaccga ctccggcttg ggcgaagacc tcgcaggtct
      241 cggtctcgaa agcgacctgc gcgagatgat cttctccacc ggtctcaaag gcaccaaagc
      301 ccgcaacgcc aaaaagatcg aaacgctcat cttcgacaca ctgcaaaaac tcgtcaacga
      361 aggcatcgac cccgatacca tcgccgcctc gatcaacacg ctcgaattcg ccatgcgcga
      421 aaacaacacc ggcggcttcc cacgcggcat cgccatcatg ctgcgcgccc tcaccacttg
      481 gctctacgat gacgacgcct tcaaaaccct cgccttcgaa gccccactcg ccgagatcaa
      541 gaatcgactc gccgccgact cgcgctattt cgaaaagatg attcagactc acctgctgga
      601 taacgtccac cgcacggtca ttcgcctcac tcctgacgtg gaactggctc agcgcctcga
      661 agcagaggaa acgctcaggc tggattctgc cagaaagacc atgagcgatg atgatatccg
      721 caagcatatt gagaacaccg agaagttgaa gatccgtcag gagactcccg acaaccccga
      781 agatgttgcc aaacttcctg tgcttgattt agaagattta gacaagcaag cccgcacgat
      841 cccaattgaa gaaattcaag tacaagactc aaaggtcttg tatcacgaca tcttcaccaa
      901 cggcattgtc tatcttgacc ttgctttcga catgcgtgcc atgccgcagg aactcctgcc
      961 gctgacggaa gtctttgcgc gcgcactgtt cgaggtgggc accgaaactg aagactacgt
     1021 caaactgtcg cagcggatcg ggaaaagcac aggcggaatc tacggctcgt cggtcacagc
     1081 caccctgcaa gggtcaagag agagccgcgc ctatctcacc atccgcggca agtccaccat
     1141 gagtcaagcc aacgagatgt tgagcatcct gcgtgatgtc ctcctgaccg tgaagttcga
     1201 caacaaggaa cgcctcaagc aaattgtgat ggaagagaaa gcgggcttgg aatctggctt
     1261 ggcgcccgcg ggacaccgct ttgcgaacat gcgcctgcgc tcgcagtttg gcggcacggg
     1321 ctacatcaac gaccagacca agggcattgg ctatctgttt gcgctgcgcg aactcgccac
     1381 cgagatcgat aaaaaatggg cgaacgtgtt gaagaagttg gagaccatcc gcgagacgtt
     1441 gatcaacagc aagaccctgc tctgtaacgt gacgctcgac gctcacaact ggaaaacctt
     1501 ccagccacat ttggagaatt tcatcttcgc catgcctgtg aaggacgttg aactttcacc
     1561 tttcaacttt gcaccttcca ctcaaaaaga aggattggca attcccgcac aggtcaacta
     1621 tgtggcgaag ggcgcgaacc tgtatgacca tggctatgag ttagatggct cttcatctgt
     1681 aattgttggc tatctcggca tgactcactt gtgggaaaag attcgtgtgc aaggcggcgc
     1741 ttatggcgcc ttcgcccaat ttgatgacgc gacgggtgta ttcacctacc tttcctaccg
     1801 cgacccgaac gtggataaca ccatcgccag ttacgacagc gccgccgcgt ttttgaaaaa
     1861 cctcgacgcc tcacgcctga acgacaacga attgaagaaa tccatcatct ccgccatcgg
     1921 taatttagac tcgtatcaac tgcccgacgc aaagggctac acttcaatga tgcgcttcat
     1981 caccggtcgc agtgatgaaa tccgccagaa gtatcgtgat caggtactct ccactaacgg
     2041 cgaagatttc atcgcattcg gcgaagtttt ggagaaagta gcgcaatcgg atgctgtcgt
     2101 ggtgctgggg tcacagtccg cgctggaggg gtcaaaggca gggctgaagg tgatgaaggt
     2161 ggcgtagaag agcagagatt agagattgga taattagaga ttagttatgg caggtttgga
     2221 agagcaatta gtgtcagaac tttgggcaag ggatgtgcgg ttcattcttg gaactgcgcc
     2281 aaatcacccg cccatgcttt cgcctgtgga attgataaaa gggcttgcag taagcgttga
     2341 aacacgtttg cagttgagtc tgattccagt gttccttcgc catcccgaat tctctattta
     2401 cgccgaatct gttgcaaaaa atctaccttt ggaaaagcag ataatactaa agtgttttta
     2461 ttgcgcggca gtatggctcg agtgcaaaca tctttcacaa aacaatctgc ctgacttatt
     2521 ttcaaaagat ttaggattat tacctacatc tgacccagat aacaatctgc ttttgcttgc
     2581 aaaccgacaa aaagagttaa gtagttctcg aatcaattgg gctgccacct atcaacatgc
     2641 agcggatatt tggttaaaag accttgagtt gcgaaagatg aaaaatggaa aaagaaactc
     2701 ttgaaagcat tctaaaagag ttcgggaagc gcgttccacc tggttcgcaa ttgcaccttc
     2761 ttggcggcgg agcgttaacc ctactaggaa gtccgcgacc ttctcttgac attgattttg
     2821 taggcgatga tgtccatccg aatgagttac atcgtacaat tctgagcatt gccaaagaaa
     2881 tggatttgtt tgttgatgca gttcccattg accgattcgt cccattacca gaaggtcacg
     2941 aaaaccgcgc cattcatata ggtcaatttg aaaatttgga aatatttgtt attgatccat
     3001 acagcattgc gttgagcaaa gtagaccgag gctcatttac cgattatgac gatatcctct
     3061 ttttgattca aaaaagatat attgatatga gcaaattgga agaaatcatc aaagaagcgg
     3121 ttgccaaagc tggcaaatat gaccttcacc ctgaaatcct tagccatttt gaagaactaa
     3181 aatcaagact ctcttaatct ctaaagtggc atagacaaac ttgaaaaaca cttccatatt
     3241 tttagtacaa taagagggca aggatattcc ttgccctctt atttcaatct ccgtaataca
     3301 taagccattg cataactctc aaggagtttt catgaacatc acactcaaga tcaatggaat
     3361 tgaacacacc atcgaagcgc ctgcttcgac taccctgctt gccgccctgc gcgggctggg
     3421 ttttcacggc gtgaagtttg gcgacgagca aggtctcacc ggcgcggata ctgtgctgct
     3481 ggatggcaaa cccatcaacg caggttccat gt
//
LOCUS       DAOQJE010000299         3506 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_17047_length_3506_cov_5.253839, whole genome shotgun sequence.
ACCESSION   DAOQJE010000299 DAOQJE010000000
VERSION     DAOQJE010000299.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3506)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3506)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3506
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17047_length_3506_cov_5.253839"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..717)
                     /locus_tag="PLT08_08885"
     CDS             complement(<1..717)
                     /locus_tag="PLT08_08885"
                     /inference="COORDINATES: protein motif:HMM:NF013740.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HNE04619.1"
                     /translation="MKKTSDQWSLPFRYAMGIIIFILVVAFLIYAREAVTMLVIAGFI
                     AYLISPAVLILKERTGWSRKVAVNVVYFTALIVLVGIPATLTPIFFDEIQLVAQDVLE
                     LSQDFSNFLSEPIQMGGLVIHLEQVGESLAHIQDSILTPLPEEALQLIEATSINILWV
                     LIILVAVHLFMSEWPKMHSWLIHLAPEPYQDDMRELYNRLRNVWMAYLRGQIVLMLVV
                     GVTFTIAWAVIGIPGALVLGM"
     gene            complement(714..1982)
                     /gene="thrC"
                     /locus_tag="PLT08_08890"
     CDS             complement(714..1982)
                     /gene="thrC"
                     /locus_tag="PLT08_08890"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004795 - threonine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HNE04620.1"
                     /translation="MNKFNGYRCSICGSEYLPGQVTYTCPKDGGNLDVVLDVDTIKKK
                     FQPEDLISRAEASLWRYLPLLPVNEPVGDSTPLHASGWTPVFALPRLAEKLHLKHLWL
                     KDESRNPTASFKDRASALVVTRARELKSEIVVTASTGNAGAALAGMAAAVGQKAVIFA
                     PKTAPQAKVAQLLVFGAKVILVDGTYDDAFDLTVKASNEFGWYCRNTGYNPFTLEGKK
                     TAAFEIWEWWIEAHRDWHKKDSTLDNHSPLTIFVSVGDGNIISGIHKGFKDLLALGWI
                     SNMPRIIGVQAEGSAAIANAFHAGTETITPVSATTIADSISVDLPRDGVRAVRAAQQT
                     DGTYIVVKDEDIIKAIAELGKIGVFAEPAGATAYAGLVKATSQGVVGSDDPVLVLNTG
                     SGLKDVRAALQAVQSAPIIEPTLEAVKKIL"
     gene            complement(2061..3059)
                     /locus_tag="PLT08_08895"
     CDS             complement(2061..3059)
                     /locus_tag="PLT08_08895"
                     /inference="COORDINATES: protein motif:HMM:NF014684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNE04621.1"
                     /translation="MNQETLLVGLAGVLAAAAPIIFAVMGETLSERAGVINLSMNGTI
                     LLTAMSGFAVAYKTDSLILGFLAGMLIGALVALVVAFASITLKQSQVAIGFVLALTCR
                     DLAYFLGNPFMGVTGPRLQATPIPGLSDIPILGPVFFRQEVITYVSFLAIFIAWLWIY
                     RTRPGLMLQGIGEKPAAAYVRGANVNLMRYVYTVLGGALIGLAGPTYSLGVRAGWMGT
                     ISGLDGIGWIALSITIFGGWNPARAAFGSYLFAFLQWLGLVLQPVLTQVPSQVLQVAP
                     FPLMILTLLLVNIGNAEWVERTLAAMPESARKFTAKLLRAMRASPPAALGVPFERD"
     gene            complement(3067..3441)
                     /locus_tag="PLT08_08900"
     CDS             complement(3067..3441)
                     /locus_tag="PLT08_08900"
                     /inference="COORDINATES: protein motif:HMM:NF014684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNE04622.1"
                     /translation="MGKNNRAAYLLGIPTWQYSLYSFLICGALAGIAGSLQVTAVYHR
                     LIPSISSGYGYLGLMVAMLVNYQAIWALPVALFFAALNIGSIQLPIVMQLDSSLSGVL
                     QGVLVLFVLLTEGARQKFGKKG"
BASE COUNT         1029 a          930 c          778 g          769 t
ORIGIN      
        1 catacccaac accaaagccc caggaatacc gatcaccgcc caagcaattg taaaggtcac
       61 acctaccact aacatcaaca cgatctgacc acgcaggtac gccatccaca cattgcgcaa
      121 acggttatag agttcacgca tatcatcctg ataaggctca ggtgccaaat gaatcaacca
      181 ggaatgcatt ttcggccatt cagacatgaa taaatgaact gccaccaaaa taatcaacac
      241 ccacaatata ttgatagaag tcgcttcgat taactgaagc gcctcttcag gcaacggcgt
      301 caaaatacta tcttgaatgt gcgccaagct ctcgcccact tgctccaaat gaattaccaa
      361 gccacccatt tgaataggtt cagaaagaaa attactaaag tcttgcgaga gctctaaaac
      421 atcttgagct accagttgaa tttcatcaaa gaagatcggg gtcaacgtgg ctgggattcc
      481 caccaacacg atcaatgccg tgaaatacac cacattgacc gctactttac gagaccaacc
      541 cgttctttct tttaatatca aaactgccgg actaatcaaa tatgcaataa aaccagcgat
      601 caccaacatg gtcaccgctt cgcgcgcata aatcaaaaac gccacgacaa gaataaaaat
      661 gataatcccc atcgcatatc gaaaggggag gctccactga tcgctagttt ttttcataaa
      721 atctttttca ccgcttctaa agttggctca ataatcggcg cagactgcac tgcctgcaat
      781 gccgctcgca catctttgag tccactgcct gtattcagga cgagaacagg atcatcgctt
      841 ccaaccacgc cctggcttgt ggctttgacc aaaccggcat atgccgtcgc cccagcaggt
      901 tcggcaaaga cgccgatctt tcccagctct gcaatggctt tgataatatc ctcatctttg
      961 acaacaatat acgtgccatc ggtctgctga gctgcccgca ctgcacggac accgtctcgc
     1021 ggcaaatcga ccgagatgct atctgcaatg gttgtggcag acacgggtgt gatggtttct
     1081 gtccctgcat ggaaggcatt agcaattgcc gcagaccctt cggcttgcac accaataatg
     1141 cgaggcatat ttgaaatcca gccgagtgcc aataaatctt tgaagccttt gtggatgcca
     1201 gagataatat tcccgtcccc tacagagaca aagatagtta acggagaatg attatccaag
     1261 gtactatctt tcttgtgcca atcacgatga gcttcaatcc accattccca gatctcaaaa
     1321 gcggcggtct tcttaccttc cagtgtgaac gggttatagc ctgtattacg acagtaccaa
     1381 ccaaattcat ttgaggcttt aacggtcagg tcaaaggcgt catcataggt tccatctacc
     1441 agaatgactt ttgcaccaaa gaccagcaat tgcgcaacct tcgcttgtgg agcagttttg
     1501 ggcgcaaaga taacggcttt ttgtccaaca gcggcagcca tgcctgctaa tgccgctccg
     1561 gcgttgcccg ttgaagcagt gactacgatc tccgatttga gctcgcgggc acgcgttacg
     1621 accaaggcgc tagctctatc tttgaacgag gcagtagggt tgcgcgactc atctttgagc
     1681 cagagatgct tcaggtgaag tttttctgcc aatcgtggca gggcaaagac cggggtccag
     1741 ccggaggcgt gaagaggcgt ggaatcgcct acgggttcat tgaccggcaa cagcggtaaa
     1801 tacctccaca gcgaagcctc ggctcgagag atgagatctt caggctgaaa tttcttcttg
     1861 atggtatcaa cgtctaagac cacatccaag ttcccgccat cttttggaca cgtatatgtg
     1921 acctgccctg gaagatactc actcccacaa attgaacaac gatacccgtt gaatttattc
     1981 atacatttcc tctagggctt aattttactc gatataaaaa tcaaaaagga ccctcatacg
     2041 agagtccttt tatgttgcaa ctaatcgcgc tcgaatggga cgcccaaagc agctggcggt
     2101 gaagcccgca tggctcgtaa taacttcgcc gtaaactttc gcgcagattc tggcatggct
     2161 gccaatgtac gttcgaccca ttccgcattg cctatattga cgagcaagag ggtgaggatc
     2221 atcaacggga agggcgcaac ctgcaagact tgcgaaggga cttgggtaag caccggttgc
     2281 aaaaccagcc caagccattg caagaaagcg aagagatatg aaccaaacgc ggcacgagcc
     2341 ggattccaac cgccaaaaat cgtgatggaa agggcgatcc acccgattcc gtccaagccg
     2401 gaaatggttc ccatccatcc agcgcggacg cccaacgaat acgtgggtcc tgctaaaccg
     2461 atcaaagcac cgcccagaac tgtataaaca tagcgcatta gattcacatt cgcgccacgc
     2521 acataggcgg ctgctggttt ttcgccaatg ccttgtagca ttagaccggg gcgcgtgcga
     2581 taaatccata accaggcaat gaagattgcc aagaagctga cataggtaat cacttcttgc
     2641 cggaaaaata caggaccgag aatcggaata tcgctcagcc caggaatggg agtcgcttgc
     2701 aaacgcggac ctgtaacacc cataaatggg ttgccgagaa agtatgccaa gtcgcggcat
     2761 gtgagcgcca aaacaaagcc aattgccact tgagattgtt tgagggtaat gctggcaaat
     2821 gcaaccacta aagcaacaag cgcaccgatc aacatgccag caagaaaacc aagaatcaaa
     2881 ctgtctgttt tataagcgac tgcaaatccg ctcatagcag tcaacagaat agttccgttc
     2941 atggataaat tgatgacgcc agctcgttca gaaagggtct cgcccatcac cgcaaaaata
     3001 atgggagccg ctgcagcaag tacgcccgcc aagccaacaa gaagagtctc ttgattcatg
     3061 gatgccttat cccttcttcc caaatttttg gcgagcccct tcagtgagca aaacgaagag
     3121 caccaaaaca ccctgcagca cacctgagag agacgaatcc aattgcatca caattggcaa
     3181 ttgaatgcta ccaatgttca acgccgcaaa gaaaagagcg actggcaacg cccagatggc
     3241 ttggtaatta acaagcatag ccaccatcaa accaagatac ccgtatccgc tagaaatgga
     3301 agggatcagg cggtgatata ccgcagtcac ttgcaaagaa ccagcgatac ccgccaaggc
     3361 tccacagatg aggaaagagt ataaagaata ttgccaggta ggaataccga gtaaatatgc
     3421 agcgcggttg ttcttgccca cggctttgat tttctagcca taataggtgc cttgcaatat
     3481 gaaatacaca ataactattg cgatca
//
LOCUS       DAOQJE010000300         3505 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_17055_length_3505_cov_6.404638, whole genome shotgun sequence.
ACCESSION   DAOQJE010000300 DAOQJE010000000
VERSION     DAOQJE010000300.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3505)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3505)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3505
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17055_length_3505_cov_6.404638"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..346)
                     /locus_tag="PLT08_08905"
     CDS             complement(<1..346)
                     /locus_tag="PLT08_08905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="N(G),N(G)-dimethylarginine
                     dimethylaminohydrolase"
                     /protein_id="HNE04623.1"
                     /translation="MTIAITRHISSRFNECEITHIERTPINLEIARSQHEAYVRALAE
                     LGCQVIELPEETELPDSVFVEDTAFILPEVAVITRPGADSRKPEVASIIPALSPYRPL
                     LHVAAPATVDGGD"
     gene            complement(350..718)
                     /locus_tag="PLT08_08910"
     CDS             complement(350..718)
                     /locus_tag="PLT08_08910"
                     /inference="COORDINATES: protein motif:HMM:NF013868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HNE04624.1"
                     /translation="MATPGFKITSEEIQHNDKSVTVFHLSGWVNVQSEEKVFSTAETA
                     YTNGSRYLVINLEDVQMLTSAGIRLLQRIYKLYRSTETIGLKICSAPSQVYHALALTG
                     LLQTVPMYENLQAALSAYDL"
     gene            complement(734..1489)
                     /locus_tag="PLT08_08915"
     CDS             complement(734..1489)
                     /locus_tag="PLT08_08915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HNE04625.1"
                     /translation="MVKVTNLDKYFGRLHVLKDISLEVPERQVLCLIGRSGSGKSTLL
                     RCLNFLEDPSRGIIEVDGIKVDAEVKNKERRQLIHDVRLKTGMVFQEFNLFPHMTVLE
                     NVIEGPVTVKGMDKKVATELAEQNLDSVGMLFKKDEYPLRLSGGQKQRVAIARALTMQ
                     PRVMLFDEPTSALDPELIGEVLNVMKKVAYEGMTMIVVTHEMGFARDVADRVIVMSEG
                     QLIEDATPEALFTSPKDPRTKALIDRYRSDDQQ"
     gene            complement(1506..2354)
                     /locus_tag="PLT08_08920"
     CDS             complement(1506..2354)
                     /locus_tag="PLT08_08920"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HNE04626.1"
                     /translation="MASTTNQEQAPLSQAELLYQAQLRKERLFRTHVGVSWAVLFFIL
                     IFLFSGQTIQLGSFKFDTIELDSSFILKNLSFIAQGLGQTLLISVLSILFAMILALLA
                     ALGRLSTFPPIYAISTFYVSLIRGTPLYLQIFFFFLALPQLGIILSGIVAGVMALGLN
                     YGAYMSETFRAGLSSVGKGQREAAMALGMTPGQTMRRVILPQALRFAIPPMGNDFISM
                     TKDSALVSATGFVHELMWRATKVGRAQFNNLEALIMAAMFYWILTLILTYFQGKLEAR
                     LAKGDR"
     gene            complement(2432..3343)
                     /locus_tag="PLT08_08925"
     CDS             complement(2432..3343)
                     /locus_tag="PLT08_08925"
                     /inference="COORDINATES: protein motif:HMM:NF012707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HNE04627.1"
                     /translation="MKKLLTLASLLAVASLVLAACGGGGGSSASDLLGAIQERGYILI
                     STDPNYEPQSFLNTEGSRPADTKCPSDALTTAEMQGFDVDVATAIGDSLGVETCFATP
                     SWDAITAGNWADKWDMSVGSMTVTTARQQILDFSVPYYYTPAVVAVAADAGIDSLEGL
                     SGQSVCAGAATTYEAWLNNDTEALGLPASSIYAQVPADVTVVPLETDQECAQAIASGR
                     KDFVAYVTSETVVDANLAAGMPVVKLDGAVYSEDLAAAFDKSSSLDTASLRAKVDELF
                     KAMHEDGRLSELSNKWFEVDLTAAPNQ"
BASE COUNT          920 a          790 c          939 g          856 t
ORIGIN      
        1 catcgccgcc gtccacggtg gcaggggctg ccacgtgtag tagtggacga tacggcgata
       61 atgctgggat gatagacgcg acttcaggtt tgcgagaatc agcgccgggt ctggtaatga
      121 cagcgacctc aggcagaata aaagctgtat cttcaacgaa gactgaatca ggaagttctg
      181 tctcttcggg aagctcaatg acctgacagc ccaactcagc tagtgcacga acatacgcct
      241 catgctgcga acgtgcaatt tccaagttta tgggtgtgcg ttcaatatga gttatttcac
      301 attcgttgaa acgagaactg atatggcggg taatggcaat ggtcatgatt tagaggtcgt
      361 atgcagaaag cgcggcttga aggttttcat acattggcac ggtttgtagc aagccagtta
      421 gagccaacgc atgatagacc tgagatgggg cgctgcaaat cttcaacccg attgtttcgg
      481 tagaacgata tagtttatag attctttgta gaaggcgtat cccagcgctg gtgagcatct
      541 gtacatcttc cagattgata acaagatagc gagagccatt tgtgtaggct gtttctgctg
      601 tagaaaaaac tttttcttcg ctttgtacgt tgacccagcc agacaagtgg aatacagtaa
      661 cagatttgtc gttatgttga atttcttcgc ttgttatctt gaaacccgga gtagccatga
      721 aaaagatgcc ttattattgt tggtcgtcgg aacgataccg gtcgatgagg gctttggtgc
      781 gagggtcctt aggactggta aacaacgctt ctggggtggc atcttcgatc aactgacctt
      841 cagacatcac gatgacacga tctgccacat ctcgggcaaa gcccatttcg tgtgtgacaa
      901 cgatcatggt cattccttcg taagccactt tcttcatcac atttaatact tcgccaatga
      961 gttcagggtc taatgcagag gttggttcat cgaagagcat cacacgcggt tgcatggtga
     1021 gggcgcgcgc aatggcaact cgctgttttt gtccgcccga taggcgcaac ggatactcgt
     1081 ctttcttgaa aagcatgcct acactatcca ggttttgttc tgcgagttca gtggcgactt
     1141 ttttatccat gccctttaca gttacaggac cttcgatcac gttctctaat acggtcatgt
     1201 gtgggaagag gttgaattct tgaaacacca tgccggtctt caaacgcaca tcgtgaataa
     1261 gttggcgacg ttctttgttc ttgacttcag catctacctt gatgccatct acttcaatga
     1321 ttccacgaga gggatcttcg agaaagttaa ggcaacgcag aagcgtgctt ttccctgaac
     1381 cgctacgccc gataagacac agaacctgac gttcaggcac ttcaaggcta atatctttca
     1441 atacatgcaa acgcccgaaa tatttatcga ggtttgtaac ttttacaatt ggaagggtgg
     1501 acatgttatc ggtctccctt tgcaaggcgg gcttccaatt tgccttggaa gtaggtgagg
     1561 atgagggtaa ggatccaata gaacatggct gccataatga gcgcttcaag gttgttgaat
     1621 tgagcacgtc ctactttggt ggcgcgccac atcaattcgt gaacgaaacc tgtcgccgag
     1681 acgagagccg agtctttcgt catggagata aagtcgttgc ccataggtgg aatggcaaaa
     1741 cgcaaagcct gcggtaggat cacgcgcctc atggtctgac cgggagtcat gcccagcgcc
     1801 atggcagctt ctctttgccc tttgcctacc gagcttaagc cggcacggaa tgtttcagac
     1861 atgtaggcac cataattcag acccaatgcc atcacccctg cgacgatacc ggaaagaata
     1921 atgccaagtt gtggcaaggc aaggaagaag aaaaatattt gcagataaag cggcgttcca
     1981 cggatcagcg atacgtagaa cgtgctaatg gcataaatag gagggaaggt ggaaagcctg
     2041 ccaagggctg ccaaaagagc taaaatcatt gcaaacaaaa tagagaggac ggagatcaaa
     2101 agagtttgcc ccaacccttg tgcaatgaaa gatagatttt ttagaataaa actgctgtct
     2161 agttcgatag tgtcgaactt aaaagaacca agttgaattg tttgcccact gaaaagaaag
     2221 atcaagataa agaacagaac agcccaggaa acgccgacat gcgtgcgaaa caaacgttcc
     2281 ttgcgaagtt gtgcctgata aagcaactcg gcttgtgaaa gcggagcttg ttcctggttg
     2341 gtggtggaag ccatctttta cctccgagag gtgaagtgga ataataaaaa gagaagcgga
     2401 gatgatccgc ttcccttttt atttcgtttt cttactggtt gggtgcagcg gtcaagtcaa
     2461 cttcgaacca cttgttggag agttcggaga ggcgaccatc ttcgtgcatg gctttgaaga
     2521 gttcgtccac cttggcgcgc agggaagcgg tgtcaaggga agaggacttg tcgaaagcgg
     2581 cagcgaggtc ttcagagtag acagcgccgt ctaacttgac gacaggcatg cctgcggcga
     2641 gattcgcatc cacaacggtt tcagaggtca cgtaggcaac gaagtcctta cgaccagagg
     2701 caatcgcctg ggcgcattcc tgatcggttt cgagaggaac cacggtgaca tcagcaggaa
     2761 cctgagcgta gatggagctg gcgggcaagc caagcgcttc tgtatcgttg ttgagccagg
     2821 cttcgtaggt ggtggcggcg ccagcacaaa cggactgacc ggaaagacct tcgagagagt
     2881 cgatgccagc atcagcagca acagccacaa ccgcgggggt gtagtagtag ggcacgctga
     2941 aatcgaggat ctgttggcga gcggtggtga cggtcatgga gcccacgctc atatcccatt
     3001 tgtctgccca gttaccggca gtgatcgcat cccaggaggg agtagcgaaa caggtttcca
     3061 caccgagtga gtcaccaatt gcggtggcaa catctacatc gaagccctgc atttcggctg
     3121 tggtgagggc gtcgctgggg cacttggtgt cagcagggcg ggagccttcg gtgttcaaga
     3181 aagactgagg ctcatagttt gggtcagtag aaatgaggat gtatccgcgt tcctgaatgg
     3241 ctccaagtag gtctgaagcg gaagaaccgc ctccgcctcc acatgcagca agaacgagcg
     3301 atgctacagc gagaaggctt gcaagggtca aaagtttctt catttttctc ctccgagaga
     3361 aataaaaatt attggcagcg atttcgctgc cacggaatgc tctccactcc gcgaggttaa
     3421 gcatagacta tttttttcaa ttgtgcaagg attttagtta ttaaaaaaca ttatatataa
     3481 attttgtgca atatcgcgca atcgc
//
LOCUS       DAOQJE010000301         3504 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_17071_length_3504_cov_4.496086, whole genome shotgun sequence.
ACCESSION   DAOQJE010000301 DAOQJE010000000
VERSION     DAOQJE010000301.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3504)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3504)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3504
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17071_length_3504_cov_4.496086"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..325)
                     /locus_tag="PLT08_08930"
     CDS             complement(<1..325)
                     /locus_tag="PLT08_08930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HNE04628.1"
                     /translation="MYRNSLKIILTVSILIGCVPASFSAETHPAPTAATSTQVGAANW
                     WGDTVFYEIFVRSFYDSDGNGVGDFNGITAKLDYLNDGDPQTNSDLGITGIWLMPIFP
                     SPSYHG"
     gene            complement(388..1290)
                     /locus_tag="PLT08_08935"
     CDS             complement(388..1290)
                     /locus_tag="PLT08_08935"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HNE04629.1"
                     /translation="MSSNPITRFYRWFFFRSRGDSPFKTLMIHAALIASCVVAVYPAL
                     RVITISLRPDDSLLTTDLRIIPENATLDNYKEVLFGAPEKNRQSDFFLWIWNSFSVVA
                     VTSIISVILAAISAYAFSRFKFPGRSVGLIFLLTTQMIPAGMLLLPLFIMIAKLKMIN
                     TSLGLIIAYAVTSVPLTIWTLKGYYDTIPVDLEEAALIDGASRFTVFTNIILPLSTPA
                     LAIAFLFSFMGGWSEYLVARVVLQKNEIYTWPLGIFTFAQQFTVSWGQFAAASVLIAI
                     PVMALFLYSSKWLISGLTLGSVKG"
     gene            complement(1303..2805)
                     /locus_tag="PLT08_08940"
     CDS             complement(1303..2805)
                     /locus_tag="PLT08_08940"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HNE04630.1"
                     /translation="MENENFLKRNLPTILWIAAFASIYYVFSSLISPFSDGITLNTAI
                     VMERLKEIGSFLFGILGGLAIIEIYFYQIFFSEPANRTAAIITRTIQAALGFLTLIII
                     LYFFNLTDLIKLGDGAISKFVSGPFEWLSSLQKSLIEAKIATKDSAGMIAGIIAGLGF
                     LILFGVIQFTSKPRNSRVGLAYLLILPAFIGVLFLIVYPFLFEIKLAFSNASLGTQAT
                     KNAEYGFMYGWENLKTLFTGPVAKDAKFFEVFWRTILWTGINVTFHVLGGMGLAILLN
                     RPLKFKGIYRTLLVIPWAMPATIAAMALRNEFDNQYGLFNILLGNLNTWLTAVSHNTE
                     NIIGIGNAFAWLAATIGPVSWKQDPFWAFVSILIANIWLGIPFMSVIILGGLQSISQE
                     YYEAAEIDGATRWQQFRNITLPLLRPVLTPAIILGVVWTFNKFDIIYLITQGGPQEKT
                     DILVSSMYKAAFQFYRYGFTAMFALVIFAILFAFTLFYLRVSDGLKSASE"
     gene            complement(2937..>3504)
                     /locus_tag="PLT08_08945"
     CDS             complement(2937..>3504)
                     /locus_tag="PLT08_08945"
                     /inference="COORDINATES: protein motif:HMM:NF024808.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HNE04631.1"
                     /translation="AQLKSDGVVPAECDYACADAMMKEGKAAMIINGDWSLGEYQTAL
                     GENFGVAPVPSINGNAYTEMTGNKAFMFSTAVLDDAAKKDAVVKFVTFMTSVEVQKRW
                     LDEFKRLPSNKEVANDPAISSDPILVASMAALSNGRGQPAAPQMRCAWDAWRPNLEGV
                     MAGTLTPEDAAAAAQEAADACVATLGTN"
BASE COUNT          981 a          781 c          933 g          809 t
ORIGIN      
        1 acccatgata agaaggggag ggaaagatgg gcataagcca gatgccggtt atcccaagat
       61 cagagttagt ttgcgggtcg ccatcgttga gataatcaag tttcgctgta attccgttaa
      121 agtcccccac tccattccca tcggagtcat aaaaggagcg tacaaagatc tcataaaaaa
      181 cggtgtcgcc ccaccaattg gcggcaccca cctgggtgga tgtggcggca gttggcgctg
      241 gatgcgtttc cgctgagaaa gaggcgggaa cacatccaat taatattgat acagttagaa
      301 ttattttgag agagtttcga tacatgcaaa gacccggggt ataaaaaggg cggtcaaaag
      361 accgcccaat attgggtgtg gattgcttta tccctttacg cttccaagtg ttaatccgga
      421 gatcagccac tttgaggagt acaggaacag cgccatgacc ggtatagcga ttaaaacaga
      481 cgctgctgca aactgccccc acgaaacagt gaattgctgt gcaaaagtaa aaatgccaag
      541 gggccaggtg tagatttcat tcttttgtaa gacaacacgg gcaacaagat actcactcca
      601 gccgcccatg aaggagaaga ggaatgcgat cgcaagggca ggtgtggaaa gtggcaggat
      661 gatgtttgtg aatacggtaa agcgacttgc tccgtcgatc aatgccgcct cttccaggtc
      721 gaccgggatc gtgtcgtaat accctttgag agtccagatc gtgagcggaa ctgaagttac
      781 tgcataggca attatgagcc ccagtgaagt gttgatcatt tttaacttgg cgatcatgat
      841 aaataagggg agcaatagca tgcctgccgg gatcatctgt gttgttaata agaatattaa
      901 cccaactgag cgcccgggaa atttgaagcg ggaaaaagcg taggcgctga tcgcggcaag
      961 gataacgctg atgatcgacg tgacagcaac taccgaaaaa gagttccata tccacagaaa
     1021 gaaatcagac tgccgatttt tctccggcgc tccaaataga acttccttgt aattatccaa
     1081 ggtggcattt tcaggaatga tccgtaggtc agtcgtaagc aacgaatcat cagggcgcag
     1141 ggatatggtg ataacgcgca atgcagggta gacagcaacg acgcacgagg caatcagcgc
     1201 ggcatggatc atcaaagttt taaacgggct gtcgccgcgg gagcggaaga aaaaccaacg
     1261 gtagaagcgg gtaatgggat ttgaggacat gatggttgtt ccttattctg atgcggactt
     1321 aagcccgtcg ctgacccgga gatagaaaag ggtgaaagcg aacaagatgg cgaaaatgac
     1381 aagtgcgaac atggcggtga aaccgtatct ataaaactgg aatgccgcct tatacatgga
     1441 ggatacaaga atatccgttt tttcctgagg tcccccctgt gtgatcagat aaatgatatc
     1501 gaacttgttg aaggtccaaa ccacacccag gataatggct ggggtgagca ccgggcgcaa
     1561 aagcggcaag gtaatattcc ggaattgctg ccagcgtgtc gcaccatcaa tttcggcagc
     1621 ttcgtaatat tcctgtgaga tgctttgcaa gccgccaaga atgatgacgg acatgaaagg
     1681 gatacccaac cagatgtttg caatgaggat ggaaacaaat gcccaaaacg gatcttgttt
     1741 ccaactcaca ggtccaattg tggctgccag ccaggcaaag gcatttccga tgcctatgat
     1801 attttcagta ttatggctga cggcagtcag ccatgtgttg agattgccca atagaatgtt
     1861 gaacaagccg tattggttat caaattcatt tcgtagcgcc atggccgcga tcgtggcagg
     1921 catagcccag ggaatgacca gtaaagtgcg gtaaatacct ttgaacttga gcgggcgatt
     1981 gagtaatatt gccaacccca ttccgcccag gacgtgaaat gtcacattga tcccggtcca
     2041 taaaatagtg cgccaaaaaa cctcgaaaaa tttcgcgtct tttgccacag gtccggtaaa
     2101 gagtgttttt agattttccc agccatacat aaaaccatac tctgcgtttt ttgtcgcttg
     2161 tgttccaaga gatgcgtttg aaaacgcaag tttgatctcg aaaagaaatg ggtatacgat
     2221 cagaaaaagg acgccaataa acgccgggag aatcagcaga taggcaagcc caacacggct
     2281 atttcgcggc ttgctggtga actggatcac accgaacagg atcagaaagc caagcccagc
     2341 aataatacca gcgatcatac cagccgaatc tttggttgca atcttggctt cgatcagcga
     2401 tttttgcaga ctgcttagcc attcgaaggg tccgctaaca aacttgctta tagctccatc
     2461 tccaagtttg ataaggtcgg tgaggttgaa aaagtaaaga atgatgatca gggttagaaa
     2521 acccaaggct gcttgaatcg tacgtgtgat aatggctgcc gttcgatttg caggttcgct
     2581 gaaaaagatc tggtaaaaat agatttcgat gattgcaagt ccacccaaaa tgccgaatag
     2641 aaaactgcct atttccttca accgttccat gacaatggca gtgttcaggg taatcccatc
     2701 ggaaaatggc gagatcaacg aagaaaatac ataatagatt gatgcaaaag ctgctatcca
     2761 taggatagtt gggagatttc gttttaagaa attttcgttt tccatcttag cctctgggtc
     2821 cctcatttgc aggtggctag tacacacctg atctcttagc aaatagaaag tagggtttta
     2881 tagaaagatg gggagcggta ccgctcccca tcttttggtt tcaagcttcg atcaaattag
     2941 ttggtgccga gggtggcaac gcaggcgtca gcggcttctt gagcggcagc ggcggcatct
     3001 tcaggggtga gggtgccagc cattacgcct tcgaggttcg ggcgccaggc atcccatgcg
     3061 cagcgcatct ggggagcggc aggttgacca cgtccgttcg aaagggcagc catggaggca
     3121 acgaggatcg ggtcgctgga gatcgcagga tcgttggcaa cttccttgtt ggagggcagg
     3181 cgcttgaatt catccagcca gcgcttctga acttcgacgg aggtcatgaa ggtcacaaat
     3241 ttgacaacag catccttctt ggcggcatcg tcaagcacgg cggtggagaa catgaaagcc
     3301 ttattaccgg tcatttcggt gtaagcattg ccgttgatgc tgggcacggg ggcaacgccg
     3361 aagttttcac caagagcggt ctggtattcg ccgagggacc agtcaccatt gatgatcatg
     3421 gcggctttgc cttccttcat catggcgtca gcgcaggcat aatcacattc cgcaggaacg
     3481 acgccgtcag acttgagctg ggcg
//
LOCUS       DAOQJE010000302         3498 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_17120_length_3498_cov_5.110078, whole genome shotgun sequence.
ACCESSION   DAOQJE010000302 DAOQJE010000000
VERSION     DAOQJE010000302.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3498)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3498)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3498
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17120_length_3498_cov_5.110078"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            72..557
                     /locus_tag="PLT08_08950"
     CDS             72..557
                     /locus_tag="PLT08_08950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE04632.1"
                     /translation="MKQNIKKLSVLAIILMLTLSACKANVSRNNDGSLSVETSVTQAQ
                     LQEIISSSIADPLIKSLTVTLQQGYVSVIGERERLNDASKTDTMSFRLDLGVSNGELT
                     ASISDAQIDGVSIEQNRVDHWNQTIANRIANIGSRNENAQLQSVSITPEQVLMTWTVV
                     R"
     gene            complement(598..1824)
                     /locus_tag="PLT08_08955"
     CDS             complement(598..1824)
                     /locus_tag="PLT08_08955"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNE04633.1"
                     /translation="MSLEQFEIQKRNFRYVLIDGVGVSIANVAAPFLPVLLTRLGASN
                     FQVGLLTSMPGVTGLILAIVVGRFLQSRSNIVPWYGLSRLLVIACYTLTGIIAFTVSG
                     QAAVIAILVIWGLATIPQTGLAVAFSVVMNAVAGPEGRYALLSRRWAIFGLTGVVVTF
                     FITRVIDRVDFPLNYGLMFMGLSLGGLISYYFSRKIELPPQTPPAPVPSSSASDSARS
                     YFSLIRSEPAFQSFTVKKFVYTSAIALGQPILPLFFVREVHATDAQIGTITMVMTMVM
                     LVGYFLWPWMSRKYSGRVVLLATTLGLAFYPALTAATVKVEWIIVYAGIAGLFQAGLD
                     LVFFDELMKTVPPEYSATFVSFSQSLQYFSAVIAPTVGTWFADSIGLGGALWVSAGLR
                     LLGFVLFLIPDRRKVT"
     gene            1927..3189
                     /locus_tag="PLT08_08960"
     CDS             1927..3189
                     /locus_tag="PLT08_08960"
                     /inference="COORDINATES: protein motif:HMM:NF012308.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serpin family protein"
                     /protein_id="HNE04634.1"
                     /translation="MKKIVMLFSLFTILLSACSSGSSAGVAESGRAREKNPDVSAADL
                     QTLVDGNNAFALDLYQTLRSENGNLILSPFSISLALAMTYAGARGETETQMADVLHFR
                     SQEQTHPAFNALDLALEENPIVLDKNQEPMQLSIANSVWSEQTFTFLPNFLDTLSVNY
                     GAGVQLMDFINSPDPSRKVINQWVSDETKDKINDLLPENSVTSDTKMVLVNAIYFKAD
                     WLSPFDANNTYDSTFKLLDGSEVTVPTMGQHLYAPYFVGDGYAALELPYAGESAVMTL
                     LVPDEGRFEEIESQLNGTMFNEVLSKLAPADVTLQMPKFKYESSFNLSDALITMGMPL
                     AFDQDHADFSGMTDQQDLYISNVIHKAFVAVDEKGTEAAAATAVIMEGAGAIMPENLL
                     VIDRPFLYFIRDVETGQILFIGRVLNPQ"
     gene            complement(3231..>3498)
                     /locus_tag="PLT08_08965"
     CDS             complement(3231..>3498)
                     /locus_tag="PLT08_08965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE04635.1"
                     /translation="SADSDLQPAQIAYLKSLGAIAGDWETGALAWVANKNAARLLILR
                     GVSDLVNEKAGEAYDNLPLFQERTDEVMQILFEQLPDWLQSIKI"
BASE COUNT         1022 a          874 c          800 g          802 t
ORIGIN      
        1 agagaattta caaactaata ttgaacagaa aacaaaggag tgggatacta caaaacattg
       61 gagaataaaa catgaaacaa aatatcaaaa aattatctgt ccttgccata atcttgatgc
      121 ttacattgag cgcttgcaaa gccaatgtgt ctcgtaacaa tgatggctct ttatctgttg
      181 aaacttctgt aacacaagcc cagttgcagg agattatttc ttcttccatt gcagacccac
      241 ttatcaagag tctgaccgtg acgctacaac agggatatgt ttcggtgatt ggcgaacgcg
      301 aacgtttgaa cgacgccagc aagaccgaca cgatgagttt caggctcgac ctcggcgtga
      361 gcaatggcga attaaccgcc tccatttcag atgcacaaat tgacggagtg tccattgagc
      421 aaaaccgcgt tgatcattgg aaccagacca ttgccaaccg catcgcaaac attggcagcc
      481 gaaatgaaaa tgcccaatta cagtctgtat cgatcactcc cgaacaagtc ttaatgactt
      541 ggactgttgt ccgataaaaa aaagaagtcc atgagatgaa ctcatggact tcttttttta
      601 agtcactttg cgccgatcag gtattagaaa gagcacaaac cctagcaatc gcagaccagc
      661 actcacccac agcgcaccac ccaacccgat agaatccgcg aaccaggtac caaccgttgg
      721 agcgataaca gcggagaaat attgaaggga ctgagaaaaa gatacaaagg tggcgctata
      781 ttccggcggg acggttttca tcagctcatc gaagaagacc aaatccaaac cggcttgaaa
      841 gagcccggca atgcccgcat atacaatgat ccactccact ttaactgtgg cagcggtcaa
      901 agcggggtaa aacgccagcc ctaatgtggt agcaagcaat accacccgtc cgctatattt
      961 gcgagacatc cacggccaga gaaagtaacc taccagcatc accatagtca tcaccatggt
     1021 gatcgtgccg atctgcgcat cagtcgcatg gacttcgcgc acgaaaaaca ggggcaagat
     1081 aggctgtccc agtgcgatgg cagaggtgta cacaaacttt ttgacggtaa aggattgaaa
     1141 agctggctcg ctgcgaatga gggagaaata actccgagct gagtcagaag ccgatgaaga
     1201 agggacagga gcgggcggag tttgaggcgg aagttctatc ttgcgtgaaa agtaataaga
     1261 aatcagtccg cccaacgaaa gccccataaa catcaagcca taattgagag ggaaatcaac
     1321 ccggtcgatc acacgcgtga tgaagaacgt tacgaccaca cctgttagac caaagatcgc
     1381 ccaacgccgg ctgagcaaag catagcgccc ttcaggtcct gccactgcat tcatcaccac
     1441 agagaaagca acggctaaac cggtttgtgg aattgttgcc aagccccaga tcacaaggat
     1501 ggcaatcact gccgcttgcc ccgaaacggt aaaagcaatg atgcctgtca gggtgtaaca
     1561 tgcgatcaca agcaaacgag atagcccata ccacggcaca atattgctac ggctttgcaa
     1621 aaaacgcccc accacaatgg caaggatcaa acccgttacc ccaggcatgg aggttagcaa
     1681 gcctacctga aaattagatg ctcccaagcg agtaagcagc acgggcaaaa atggcgcggc
     1741 gacatttgca atactcacgc ccacgccgtc gatcaataca tagcgaaagt tgcgtttttg
     1801 gatttcaaat tgttcgagag acataggaaa aatgggtgag acagaatttc gattataacg
     1861 ctcccctcca tggatacaat ggaaacaaat acacactggg cggcgtccgt gtgtaaagga
     1921 gaaatcatga aaaaaattgt gatgttgttt tcgcttttta ccatcctgct ctctgcttgt
     1981 agcagtggaa gttccgccgg ggtggcagag tctggtcggg cacgagaaaa aaatccagat
     2041 gtaagcgctg cggatttaca aacgttagta gatggcaata atgcttttgc tttggatcta
     2101 taccaaacgc ttcgttctga aaacgggaac ttgatcctct ccccgttcag catttccctc
     2161 gccttagcca tgacgtacgc cggcgcccgc ggcgagactg aaactcaaat ggcagatgta
     2221 ctgcacttca ggtctcaaga acagacccac cctgctttca atgccttaga cttggcgctg
     2281 gaagaaaacc ccattgtgtt agataaaaac caagaaccca tgcaattgag cattgccaat
     2341 tccgtatggt ctgaacagac cttcaccttc ctcccaaatt ttctggatac cttatcggtc
     2401 aactacggcg caggcgttca attaatggac tttatcaata gccccgatcc ttcgcgcaag
     2461 gtcatcaacc aatgggttag tgatgaaacg aaagataaaa tcaacgatct gcttcccgag
     2521 aattcagtca catcagatac caagatggtc ttggttaatg ccatttattt caaagccgat
     2581 tggctctcgc cctttgacgc taataacacc tacgacagta cattcaaatt attggacggc
     2641 tctgaagtca ccgtgcccac tatgggacag cacctttatg caccatactt tgtcggcgat
     2701 ggatatgcgg ctctggaact cccctatgcc ggcgaatctg cggtgatgac tttgctcgtg
     2761 ccagatgaag gtcgctttga ggagattgaa tcgcagctga atggcaccat gttcaatgaa
     2821 gtcttgtcaa agctcgcccc tgcggatgtc acattacaaa tgccgaagtt caaatatgaa
     2881 agttcgttca atctctcaga tgcactaatc acgatgggca tgcctctcgc ctttgatcaa
     2941 gaccacgccg atttctccgg catgaccgat cagcaagacc tatacattag caatgtcatt
     3001 cataaagcct ttgtggcggt agatgaaaaa ggaaccgaag ccgctgctgc tacagcagtc
     3061 atcatggaag gagcaggcgc gatcatgccc gagaatttac tggtaatcga ccgtccgttc
     3121 ttgtacttta ttcgcgatgt agaaactggt cagattttgt tcattgggcg ggttttgaat
     3181 ccgcagtaaa acttgaggtg acagccgctt tgaaagtgac tgtcaccttg ctatattttt
     3241 atactttgca accaatcggg caactgttca aaaagtattt gcattacttc gtctgtccgc
     3301 tcttggaata agggtaaatt atcgtaggct tcccccgctt tttcattcac caagtcactc
     3361 acgccgcgca aaattaacaa acgcgcggcg tttttgtttg ccacccatgc caacgcccct
     3421 gtttcccaat caccggcaat ggcacccaat gatttgaggt acgcaatctg cgcgggttgc
     3481 aagtcactgt cggcagag
//
LOCUS       DAOQJE010000303         3468 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_17361_length_3468_cov_6.125110, whole genome shotgun sequence.
ACCESSION   DAOQJE010000303 DAOQJE010000000
VERSION     DAOQJE010000303.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3468)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3468)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3468
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17361_length_3468_cov_6.125110"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..886
                     /locus_tag="PLT08_08970"
     CDS             <1..886
                     /locus_tag="PLT08_08970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE04636.1"
                     /translation="GFLVIMGPWYLRNLSVFGAIMSPAGSRALWLTTYEQTFIYPASK
                     LNLQSWLASGWSEILKVRWWSFTNNIQTVIAAQGHLILFPFIAIGMYLQRKDRRVQIG
                     LLAWVILFLVMTFIFPFAGTRGSFFHAAAALQPLLLVLAPFGLEKVVAWARSKGRFNE
                     YAYVVFRVAMIQIVIMLSAWVVWVRVIQNGWEEGELSYPAVEAYLVEHGAQPDERIMV
                     LSSPGYYMMTGRPAFAQPDGDINTLLEVADRYDIQYFAFEAQGKLKPIADLYDNPQNY
                     PQFEYLGEVDDTRIFKFP"
     gene            858..>1831
                     /locus_tag="PLT08_08975"
     CDS             858..>1831
                     /locus_tag="PLT08_08975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE04637.1"
                     /translation="MTHAFSNFHKPLSKRDLVILGVVTLLGGIIYLIASQIVYRIGFP
                     LDDSWIHLTFARNLAYHGEWSFRLGVPSAGSTSPLWSALLAIGFILHLEPYIWPYFLG
                     LLTMWALSVISEVTAQRLISTYRPAFPWVGIFIIIEWHLLWAAMSGMETLLHALIATV
                     VISAIMMNSRRYLTLGLLVGISVWVRPDGLTLLGPLFLAVLLNEKDTPARLKALLQVL
                     VGFGAVFLFYLLFNLLIGGTPMPNTFYAKQAEYANWQQMPILERLGELLLQLLVSPAF
                     LLLPGMVLWVMKAIKTRDWGSLLALAWSVGYMIIYLLRLPVYQHGRYI"
     assembly_gap    1832..1931
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1932..2497
                     /locus_tag="PLT08_08980"
     CDS             <1932..2497
                     /locus_tag="PLT08_08980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE04638.1"
                     /translation="MIIYLLRLPVYQHGRYIIPAMPIFFLIGLLGMFSYVTVKREGRN
                     FWMISTSWNLSLAMVTLLFIVLGARAYARDVAFIEQEMVAAAKWAAANIPPDAILAVH
                     DIGAMGYFDEHQLIDLAGLITPEVIPFIRDEDRLAAYLDDKGADYLVAFADLYPQMEV
                     GREVVFVTNGAIATEADWVRMTIYRWK"
     gene            2564..3259
                     /locus_tag="PLT08_08985"
     CDS             2564..3259
                     /locus_tag="PLT08_08985"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HNE04639.1"
                     /translation="MNQITLLVVDDHPLFRQGVVDALSLETDMRIIAQSASGDEALEF
                     IRTKKPNVAVLDVNLPGMNGQQITHQVSQDKMNTRIILMTGYDDIEQAIHAALAGAHG
                     YCSKDIEPQNLARIIREVAEGKFVFANNVFTRRELELWVEESMEGARRSYSEPGAPFH
                     PLSDREMEVLACVVRGMSNKEIASLLGISHQTVKNHVTAILRKFGVEDRTQAVVYALK
                     RGWVTLRDTDIRS"
     gene            3269..>3468
                     /locus_tag="PLT08_08990"
     CDS             3269..>3468
                     /locus_tag="PLT08_08990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE04640.1"
                     /translation="MSETVDYKSELDETQRALREITLMIEQSQGELSKLTQRNTAITS
                     HLQQVQKQGAAMEEIKMAYDSA"
BASE COUNT          777 a          833 c          800 g          958 t
ORIGIN      
        1 tggctttctc gtcattatgg gtccgtggta tctccgcaac ctgagcgtct tcggtgccat
       61 catgtccccg gcaggcagcc gcgccttatg gctcaccacc tacgaacaga cctttatcta
      121 ccccgcttcc aagctcaacc tgcaatcttg gctcgccagc ggctggagtg agattctcaa
      181 agtccgctgg tggtcgttca ccaacaacat ccaaaccgtc atcgccgctc aagggcatct
      241 catcctcttt ccttttattg ctatcggcat gtacctacaa cgtaaagacc gccgtgtgca
      301 aataggtctg ctggcttggg tgattctgtt cctggtgatg acctttattt tccccttcgc
      361 cggcacccgc ggcagtttct tccacgcagc ggcagctctt caacctttgc tcttggttct
      421 cgcacccttc ggcttggaga aagtcgtagc atgggcgcgc agtaaaggtc gcttcaatga
      481 atatgcttat gtggtcttcc gcgttgccat gatccagatc gtcatcatgc tttcggcgtg
      541 ggtcgtctgg gtgcgcgtca tccagaacgg ttgggaagaa ggggagttgt cctatcctgc
      601 cgtggaagct tatcttgtag aacacggcgc tcaacccgat gaacgcatca tggttctcag
      661 ttcccccggc tactatatga tgacaggtcg ccccgccttt gcccaacccg atggtgatat
      721 caacaccctg ctcgaggttg ctgatcgcta cgacattcaa tactttgctt ttgaagcgca
      781 aggcaagctc aagcccatcg ccgacctgta cgacaatccg caaaattacc ctcaattcga
      841 atatctaggt gaagttgatg acacacgcat tttcaaattt ccataagccg ctctccaagc
      901 gcgaccttgt aatcctcggc gttgttacct tactgggcgg catcatttat ctgatcgcca
      961 gccagatcgt atatcgcatc ggcttccccc tcgacgactc atggatccat ttaacctttg
     1021 ctcgtaacct tgcctatcat ggcgaatggt catttcgttt aggtgtccct tcagcaggct
     1081 ccacatctcc gctctggtct gccttgcttg ccatcgggtt catccttcac cttgaaccct
     1141 atatctggcc ctacttcttg ggtctgctca ccatgtgggc attgagcgtt attagtgaag
     1201 tcacagcgca gcgcctgatc tcaacctatc gccctgcttt cccttgggta ggcatcttca
     1261 ttatcataga gtggcatttg ctctgggcag ccatgtctgg catggagact ctcctccatg
     1321 cactgatcgc cactgttgtt atatctgcca tcatgatgaa ttcgcgtcgc tacctaacct
     1381 tagggctgtt agttggcatc agtgtctggg tccgccccga cggcttgaca ttactcggtc
     1441 cgctattttt agcagtccta ttgaatgaaa aggatactcc tgcgcgcctg aaagccttat
     1501 tacaagtcct tgttggtttt ggggctgtgt ttctctttta cttattgttt aacttgctaa
     1561 ttggcggcac tcccatgcca aacacctttt atgctaaaca ggcagaatat gcaaattggc
     1621 agcaaatgcc aatccttgaa cgtctcggtg aattattgct tcagttatta gtgagcccgg
     1681 catttttatt gcttcccggt atggttttat gggttatgaa agcaataaag acccgcgatt
     1741 ggggatctct tttagcatta gcttggtctg tagggtatat gattatttat cttctgcgcc
     1801 ttcctgttta tcaacacggg cgttatatca tnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1861 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1921 nnnnnnnnnn natatgatta tttatcttct gcgccttcct gtttatcaac acgggcgtta
     1981 tatcatccct gctatgccaa tcttcttctt gatcggactt ttagggatgt ttagttacgt
     2041 caccgttaag agagaagggc gaaacttctg gatgatttct acatcctgga atttgagtct
     2101 tgctatggtg acgttattat ttattgtctt aggagcaaga gcatacgcaa gagacgttgc
     2161 ctttatcgag caggaaatgg tcgccgctgc aaaatgggca gccgctaaca tcccgcccga
     2221 tgcgatcctt gccgttcatg atatcggtgc catgggttat tttgatgaac accaactcat
     2281 agacttggct ggtttgatca ccccagaagt cattcccttt atccgtgatg aagaccgcct
     2341 cgccgcctac cttgatgata agggtgcaga ctatcttgtt gctttcgcag acctttaccc
     2401 ccagatggag gtcggtaggg aagtggtttt tgtaaccaat ggggcaattg ctacagaagc
     2461 agattgggtt cgcatgacaa tttaccgttg gaagtgaaag aatgggttaa aatatggcga
     2521 atgttaggat tgaaaacctt ctaaaaagag agaatgctaa gcgatgaacc aaattacatt
     2581 actagtcgtt gatgatcacc cattattccg ccagggcgtg gtggatgctc tgtcattaga
     2641 aacagatatg cggatcatcg ctcagtctgc gtctggggat gaggcgctgg agtttatccg
     2701 caccaagaaa cccaatgttg ccgttttaga tgtgaacctt ccaggcatga atggtcagca
     2761 gattacacat caggtctcgc aagataagat gaacacccgc atcattttga tgaccgggta
     2821 cgatgatata gaacaagcga tccatgccgc attggctggt gcccacggat attgctctaa
     2881 agatatcgag cctcagaatt tggctcgcat cattcgcgaa gtagctgaag gtaaatttgt
     2941 atttgcgaat aatgttttta ctcgtcgtga acttgaatta tgggtggaag aaagcatgga
     3001 aggtgcccgc cgttcctata gtgaacctgg tgcgccattc catccgcttt ctgatcgtga
     3061 aatggaagtc ttggcttgtg tggtacgcgg tatgagcaac aaggaaattg cctcgctgtt
     3121 gggtatcagt caccaaacag tgaagaacca tgtcacagca attttacgta agtttggggt
     3181 ggaagaccgt actcaagctg ttgtgtacgc attaaaacgc ggttgggtta ccctgcggga
     3241 taccgatatc cgctcttagg agaattagat gtccgaaact gtggactata aaagcgaatt
     3301 agatgaaaca cagcgcgcct tgcgtgaaat cacgttgatg attgagcaaa gccaaggcga
     3361 gttaagcaaa ctaacacagc ggaatactgc tatcacatct catcttcagc aagtccagaa
     3421 acagggcgct gcaatggaag agattaagat ggcttacgat tctgcatt
//
LOCUS       DAOQJE010000304         3465 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_17390_length_3465_cov_5.698240, whole genome shotgun sequence.
ACCESSION   DAOQJE010000304 DAOQJE010000000
VERSION     DAOQJE010000304.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3465)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3465)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3465
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17390_length_3465_cov_5.698240"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1164
                     /locus_tag="PLT08_08995"
     CDS             <1..1164
                     /locus_tag="PLT08_08995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE04641.1"
                     /translation="QDIQDSVGSWKWFENPESFALVGSTINPRAAGFVAGVEYQSYFR
                     PNFSSYGPQAFANTKAIGANFAVLSPSWTISNTSPLRFSAQPGNDPFWIDTAIMVSQA
                     RALGLNIALFPTPHFPPSTDSNISASEQFWKNAPKDAAWWQTFFTRYRAFLVNYADLT
                     AQSGAQVIILGGDWITPALPGGLLPDGTSSNVPADAEAQWRSILQDVRSHFKGQILWA
                     LPYEVSSFTTPVSFLRDVDGIYLLWSTPIATSSTATKTDYANETGRLLDNEVAPLASL
                     LVKPVILAVSYPSAAGAPTGCISNGSGACLDFDALSRPNVDLSSVSLNLQTQADIYEA
                     MLNAVNARPWISGFVSRGYYLPVVLQDKSTSIHGKPAADVLWYWYPRLLGTVQ"
     gene            1277..>3465
                     /locus_tag="PLT08_09000"
     CDS             1277..>3465
                     /locus_tag="PLT08_09000"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013927.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016811 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear amides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin acylase family protein"
                     /protein_id="HNE04642.1"
                     /translation="MKKIGRFLGRMLIGILIIAVVVASGGLFYFKSYLPKTVAPKSFP
                     QIDGEIKVDGLDGEVNIYRDAMGIPHIYATTAHDIFFAQGYVHAQDRFWQMDAWRHIG
                     SGQLSEMFGEGQVETDAFLRTLGWRKTAEAEWEQLDSDSRAILQSYTDGVNAYLKDHS
                     GTALSLEYAVLGLLSPDYVIEPWEPVNSLTWGKAMAWDLRGNMGEEIERAELLKTLTP
                     EQVALLYPEYPQDHPVIVNKTGEGGQSASTSQPSSFDYSALNLDSLSHNASLLDPVLG
                     PLGDGIGSNSWAVSGALTDTGKPYLANDPHLGIQMPSIWYQVGLHCVEKTDSCPFEVA
                     GFSFAGVPGVVIGHNDKLAWGFTNVGPDVMDLYIEKVNPENPNQYEVNGKWVDFETYE
                     ETINIVGGEPVTITVKKTRHGPVISDTYGPTKNVGDPEDTKFIPFKDRVGGVELPEQY
                     VIALAWTALTPSTPFEAIWGFNKAQNWEDFRKAASDFHVPAQNLLYADVEGNIGYQMP
                     GDIPIRKNGDGTVPVPGWTDDYEWEGFIPFDELPYALNPEEGYIVTANNQVNPWDYPY
                     LITKDWDYGYRAQRIVDMIKNAPGKIDKEYIQSMQGDATDLNAESIVPVWKEIDYKAA
                     TANEAYALDQLQNWDYTCKADSKACAIFQWFWWNLDQNTINDDVPERNAVSGGSKDFE
                     TFRHLLPETDNFFWDDKLTTDVTETRDDIFIASLTETVQQLEKEYGK"
BASE COUNT          932 a          854 c          798 g          881 t
ORIGIN      
        1 caggatattc aggatagtgt tggttcgtgg aaatggtttg aaaacccaga aagttttgcg
       61 ctggttggta gcacaatcaa cccgcgagca gcaggctttg tagctggcgt agaataccaa
      121 tcctatttcc gcccaaactt ttcttcgtat ggtccccaag catttgctaa tacaaaagcc
      181 atcggtgcca acttcgctgt actctccccg agttggacaa tttcgaatac atcccctttg
      241 cgcttttctg ctcagcccgg taatgacccg ttctggatcg atacagccat catggtgtcg
      301 caagcgcgcg ctttaggctt aaatattgcg ctcttcccaa cccctcattt cccgccatcc
      361 acagacagca atatctctgc cagcgaacaa ttctggaaga acgctccaaa agatgcagct
      421 tggtggcaaa ctttcttcac acggtatcgt gccttcctag tcaactatgc agatctcact
      481 gcccaaagcg gtgctcaggt tatcatcctt ggtggagatt ggatcacgcc tgctctccct
      541 ggtgggcttc tgcccgatgg cacatcttca aacgtcccgg cagatgctga ggcgcaatgg
      601 cgctccattc tacaagatgt acgctcgcat ttcaaaggtc agatactatg ggcattacca
      661 tatgaagttt ccagcttcac tacccctgta agtttcttgc gtgatgtaga tggaatttac
      721 ttgttatggt ctaccccaat cgcaacaagt tccacagcaa caaaaacaga ttacgcgaat
      781 gaaaccggtc gcttgctcga taatgaggtc gctccgctgg catctttatt ggtcaagccg
      841 gtcatccttg ccgtctcgta tccatccgca gcaggcgcgc caacagggtg catatctaac
      901 gggtctggag cctgtttaga ctttgatgcc ctctcccgcc ctaatgtaga tttatcatct
      961 gtcagtctta acttacaaac ccaggcagat atttacgaag ccatgctcaa tgcggtcaac
     1021 gcccgaccat ggatttctgg gtttgtcagc cgtggatatt atctccctgt tgtattgcaa
     1081 gataaatcca catccattca tggcaaaccc gcggcagatg tgttgtggta ttggtacccc
     1141 aggttgttag gaaccgttca ataatttatt ttcttcattc atgaatcaga cgcagagtga
     1201 gctacgaaac tctgcgtctg gtgtattaag tactgccaca gagcccatga ctctgtggaa
     1261 aaccattgga ggaactatga aaaaaattgg caggttttta gggaggatgt taatcggaat
     1321 tttgatcatc gctgtggtgg tcgcctcggg cgggcttttt tatttcaaaa gctatttacc
     1381 gaaaacagtc gcgccaaaat cttttccaca aattgacgga gagataaaag ttgacggctt
     1441 ggacggagaa gtgaatattt atcgcgatgc gatgggcatt ccgcacatct atgcgaccac
     1501 agcacacgat attttctttg ctcagggcta tgttcatgct caagaccgtt tctggcaaat
     1561 ggacgcctgg cgtcatattg gttcagggca actctctgaa atgttcggcg aaggtcaagt
     1621 agaaacagat gccttcttac gaacgttggg atggcgcaaa acagcagaag ctgaatggga
     1681 acaacttgac tctgattcac gtgccatctt gcaatcatat accgatggtg tgaacgctta
     1741 tctcaaagat catagcggaa ccgccctcag cttggaatat gccgtgttgg gtttgctcag
     1801 ccccgattac gtcattgaac cgtgggaacc tgtcaattcg ttaacatggg gcaaagcgat
     1861 ggcatgggat ctccgtggca acatgggtga agaaatcgaa cgtgcagaat tgttaaaaac
     1921 tctcacacca gaacaggttg cattgcttta cccagaatat cctcaagacc atcctgtcat
     1981 cgtgaacaaa actggtgaag gtggacaatc tgcatcaact agtcaacctt caagctttga
     2041 ctattctgcc ctcaatcttg attcactctc acacaacgcc tcattgctgg acccggtact
     2101 tggtccgctt ggcgatggaa ttgggtcaaa ctcttgggct gtctccggcg cattgaccga
     2161 caccggcaaa ccttatctcg ccaatgaccc gcatcttggc attcaaatgc cgtctatttg
     2221 gtatcaggtc ggtttgcatt gcgtcgaaaa aacagatagc tgtccgttcg aagtagctgg
     2281 cttctcattt gctggggttc ccggtgtagt aatcggtcat aacgacaaac tcgcatgggg
     2341 ctttacgaat gtaggaccag atgtgatgga tctgtacatc gaaaaggtca acccagaaaa
     2401 ccccaatcaa tatgaggtca acggcaaatg ggttgatttt gaaacatacg aagaaaccat
     2461 caacattgtc ggtggtgaac cggtaactat cacggtaaaa aagacccgcc acggaccggt
     2521 catctcagac acttacggtc caactaaaaa tgttggcgac ccagaagaca cgaagttcat
     2581 tccattcaaa gaccgtgtag gcggagtaga actgcccgaa caatacgtga tcgccctcgc
     2641 atggacggct ttaaccccat ccacaccatt tgaagccatt tggggtttta acaaggcaca
     2701 aaactgggaa gacttccgca aagccgcgag cgacttccat gtgcctgctc agaacttgct
     2761 gtatgccgat gtagaaggaa acatcggtta tcaaatgccg ggcgatattc caattcgaaa
     2821 gaatggtgac ggaactgtgc cagttcctgg ttggacggat gattacgaat gggaaggttt
     2881 tattccgttc gatgaattgc cctacgcgct caaccctgaa gaagggtata tcgtcaccgc
     2941 aaacaatcaa gtcaacccat gggattatcc atacctcatc accaaagatt gggactatgg
     3001 ttatcgtgct caacgcattg tagacatgat caagaatgcc cccggcaaga ttgataaaga
     3061 atacattcaa tctatgcagg gtgatgcaac agacctaaac gccgaaagca tcgttcctgt
     3121 ttggaaagag atcgattaca aagccgccac cgcaaacgaa gcctatgcac tcgaccagtt
     3181 acagaattgg gattacacct gtaaagctga ctctaaagcc tgcgcaatct tccaatggtt
     3241 ctggtggaat cttgaccaga atacgatcaa cgatgatgtg cccgaacgca atgctgtcag
     3301 cggcggctca aaagatttcg aaaccttccg tcatcttttg ccagaaacag ataacttctt
     3361 ctgggacgat aaacttacaa ccgatgtgac tgaaactcgc gatgatattt tcattgcatc
     3421 gttaactgag actgttcagc aattagaaaa agaatacggg aaaga
//
LOCUS       DAOQJE010000305         3464 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_17396_length_3464_cov_8.145204, whole genome shotgun sequence.
ACCESSION   DAOQJE010000305 DAOQJE010000000
VERSION     DAOQJE010000305.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3464)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3464)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3464
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17396_length_3464_cov_8.145204"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            150..1130
                     /locus_tag="PLT08_09005"
     CDS             150..1130
                     /locus_tag="PLT08_09005"
                     /inference="COORDINATES: protein motif:HMM:NF025616.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4349 domain-containing protein"
                     /protein_id="HNE04643.1"
                     /translation="MKRLTPYSLILIAMIVLGACASAPRAVELSGGSAPASEPYYDQS
                     SVMPAPTMMYATDGIIAEEAQVSGEGSGNANAAQVERIVIQNADIAVVVSDVELRMDE
                     IQKMAVDLGGFVVSSNLYQSYTSDYTPVPEASLTIRVPAENLDEALEKIKKDAVEVQN
                     ETRSGQDVTAEYVDLKSRLKTYQAAEEELTQLMDNAQNSDEVVNIFNQLMYYREQIEI
                     IQGQIKYYEEAAALSAISIRLIAEETTKPVTIGKWEPKGVALEAIQDLLDFWKGFVDF
                     MIRFVIFTLPMLITIGIPFYLAFIGVRWVFRKMRGNKKKVEPQVKEEEKK"
     gene            1190..1900
                     /gene="rsmG"
                     /locus_tag="PLT08_09010"
     CDS             1190..1900
                     /gene="rsmG"
                     /locus_tag="PLT08_09010"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: protein motif:HMM:TIGR00138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA];
                     GO_process: GO:0046118 - 7-methylguanosine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HNE04644.1"
                     /translation="MDSLIQDAKTLFNVHLTARQVVALVNYEKELMEWNQKFNLTAIR
                     DVESIRTKHFLDSYSCVLAWKANPPQRLIDVGTGAGFPGIPLKIIYPTMQVTLVESVG
                     KKAMFCQHVIQTLGLEGIEVLHSRAEDVGQNPAHRETYDWAVARAVANLNILSEYLLP
                     LVKLGGTMLAQKGETGPVEAQAAEKAMKILGGKLKQLIPVNLPGVVDDRYLVLTDKVA
                     ATPPKYPRKAGIPMKTPL"
     gene            complement(1908..2273)
                     /locus_tag="PLT08_09015"
     CDS             complement(1908..2273)
                     /locus_tag="PLT08_09015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE04645.1"
                     /translation="MEWHIEYIPEIALLYVKTKGILTNEKANQMVAEIALAMKEHQCL
                     CQIVDHRETVFDFRTLDYYERPKVNQQLGMSKQWRVAMIFKQLSEETLFMETVFRNRG
                     FEFRQFDDLDKAKEWVLKK"
     gene            complement(2373..2705)
                     /locus_tag="PLT08_09020"
     CDS             complement(2373..2705)
                     /locus_tag="PLT08_09020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE04646.1"
                     /translation="MKLSLKHYGIILGALLAAYFHLILFPDIGFTLNGLGYLGLLGAY
                     FLPIAFFQQKHKMVWWGLVGYTILTIVLWAIMGNKQFVAGTTSAIGYYAKAAEVFLLA
                     CLWADKPQ"
     gene            2909..3334
                     /gene="ssb"
                     /locus_tag="PLT08_09025"
     CDS             2909..3334
                     /gene="ssb"
                     /locus_tag="PLT08_09025"
                     /inference="COORDINATES: protein motif:HMM:TIGR00621.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HNE04647.1"
                     /translation="MYHTIIIVGNVGKDPEMRYTPSGQAVTSFSVATNRQYTAGNGEQ
                     VKETIWFRVSTWGKTAEICNQYVKKGSKVLIEGRLTPDKATGGPRIWSKQDGTAGASF
                     EVTAQTVRFLSSRDDGASGPIAGGGMDMAEIPPEDDIPF"
BASE COUNT          988 a          870 c          777 g          829 t
ORIGIN      
        1 gaaggttggt aacgcctccc acaaaagtcc tccctgaagt tcgggagggc tttttcttta
       61 taggaagttg tacaatacgt gggaacaatt tattttagcg tagcgtcctt tgaagagaga
      121 aattaccaaa ggaaaagaag gagaaagaaa tgaaacgcct aacgccctat agtcttattt
      181 tgattgcaat gatcgttctt ggagcttgtg ccagtgctcc gcgcgccgtg gaactttctg
      241 gcgggtctgc cccagcttcg gagccctatt atgaccaatc ctctgtaatg cccgcaccga
      301 ccatgatgta tgctactgat ggcattatcg ctgaagaagc ccaagtaagt ggagaaggca
      361 gcgggaacgc caatgccgct caagtggaac gtattgtcat tcagaatgca gatattgcag
      421 tcgtcgtatc agatgtcgaa ctgcgtatgg atgagatcca aaaaatggca gtcgatcttg
      481 gcggattcgt agtgtcttcc aatttgtatc agagctatac cagtgattac accccggtgc
      541 ctgaagccag cctgaccatc cgcgtgcctg ctgaaaatct ggatgaagcg ctggaaaaga
      601 tcaagaaaga tgcggtggaa gttcagaacg aaacccgctc tgggcaagat gtcaccgctg
      661 aatacgtgga cttaaagtcc cgcctcaaga cctatcaagc cgctgaagaa gaactgaccc
      721 aattgatgga caatgcacaa aactcagatg aggtcgtcaa tatcttcaat cagttaatgt
      781 actaccgcga acagatcgag atcattcagg gtcagatcaa gtattacgaa gaagctgccg
      841 ctctctctgc cattagcatt cgtctcattg ccgaagagac caccaaacca gtaacgattg
      901 gcaaatggga accgaagggt gttgcgctcg aagccataca agacctgctc gatttctgga
      961 aaggcttcgt cgatttcatg atccgcttcg taatctttac tctgcccatg ttgatcacca
     1021 tcggtattcc gttctacctg gcattcatcg gcgtccgctg ggtcttccgt aagatgcgtg
     1081 gcaacaagaa aaaagttgaa ccgcaggtga aagaagaaga aaagaagtaa aacgtcgtaa
     1141 cacgagagga cggtgtgccg ccgtcctctt tgttttataa tcttcgcata tggactctct
     1201 gattcaagac gcaaaaacat tattcaacgt ccacctcacg gcgcgacagg tggtggcgct
     1261 cgtcaattac gaaaaagaat tgatggagtg gaaccaaaaa ttcaacctta ctgccattcg
     1321 ggatgtagag tctattcgta ccaagcattt tctggattca tattcctgtg tgctggcgtg
     1381 gaaagccaac ccgccgcaac gcctcatcga cgtgggcacg ggcgctggtt tccccggcat
     1441 tccgcttaag attatttacc ctaccatgca agtcaccttg gttgaatcag tcggcaagaa
     1501 agccatgttc tgtcagcatg tgattcaaac gctgggcttg gaaggcatcg aagtcttgca
     1561 ttctcgtgcc gaagatgtag gtcaaaaccc cgctcatcgt gaaacctacg actgggctgt
     1621 ggcacgggct gtggcgaatc tcaacatctt gagcgaatat cttctgccgc ttgtcaaact
     1681 cggcgggacg atgcttgccc aaaaaggcga gaccggaccc gttgaagcac aggctgccga
     1741 gaaagcgatg aaaattttag gtggaaaatt aaagcaattg atccccgtca acttgcccgg
     1801 tgtagtagat gatcgttatc tggttctaac agataaagtc gctgctacac caccgaagta
     1861 tccccgcaag gcaggcatcc ccatgaaaac gcctttatag cttgcgccta ttttttcaaa
     1921 acccactctt tggctttatc caaatcatcg aactgacgaa actcaaatcc acgattccga
     1981 aaaacagttt ccataaacaa ggtttcttca gagagttgtt tgaatatcat ggcgacacgc
     2041 cactgtttag acatccccaa ctgttgattt acttttggac gttcataata atcaagggtt
     2101 ctgaaatcaa aaaccgtctc acgatgatcc acgatctgac agagacattg atgttctttc
     2161 attgcaagcg caatctcagc aaccatctga tttgcttttt catttgtaag tatccccttg
     2221 gtctttacat ataaaagggc gatctcgggg atatattcaa tatgccattc catacggcta
     2281 ccgcctaaaa agaagcatgg tatgacaata aagtcatacc atgttatgaa ctactacaat
     2341 agcggcggat tttaccagat aacgcaaaaa gattactggg gtttatctgc ccaaagacac
     2401 gctaaaagga aaacctcagc cgccttggca tagtatccaa tggcactggt cgtccctgca
     2461 acgaattgtt tgtttcccat aattgcccat agcacgatgg tgagaatggt ataaccgacc
     2521 aaaccccacc aaaccatttt atgtttctgc tggaagaagg caatcggtag aaaataagct
     2581 ccgagcagcc ccagataacc taatccattc agggtgaagc caatatcagg gaaaaggata
     2641 aggtggaagt acgccgccaa caatgcccct agaatgatcc cgtagtgctt aagacttagt
     2701 ttcatgcttt aactccttgg gtgattggaa ttacgacgaa tacttaacgc atggacaccc
     2761 cacctcagcc tgttcttaca tgcgtgtatt ttcacgataa aacccaaatt gattaacaaa
     2821 taaagcaaga aagagtacaa tacaaatgtt tcaattgttt cccgtcgaaa ttcggcgggc
     2881 acctttaacc caagaggaga atccccccat gtatcacact attattatcg taggtaacgt
     2941 cggtaaagac cccgaaatgc gttacacccc atcgggtcag gctgtgacct cgttctcggt
     3001 cgctaccaat cgacagtaca ctgccggaaa tggcgaacag gtcaaagaaa ccatttggtt
     3061 ccgtgtttcc acctggggca agaccgcaga aatctgcaat cagtacgtca agaaaggctc
     3121 caaggttttg atcgaaggac gcctcactcc agacaaagcc acgggtggtc cacgcatttg
     3181 gagcaagcaa gacggcaccg ccggtgcatc ctttgaagtc accgcacaaa ccgttcgctt
     3241 cctctcctca cgcgatgatg gcgccagcgg acccatcgca ggcggcggca tggacatggc
     3301 ggaaattccc cccgaagacg atattccatt ctaatcacct aaccctcatt accacagagc
     3361 ccacaaccta tacagagaac gcaagacctt tctccgtgaa cccgtgatct ctgtggtaaa
     3421 aaatttaatc tcatgaccaa tccccaaact cccccaaaac aacc
//
LOCUS       DAOQJE010000306         3458 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_17449_length_3458_cov_6.203644, whole genome shotgun sequence.
ACCESSION   DAOQJE010000306 DAOQJE010000000
VERSION     DAOQJE010000306.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3458)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3458)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3458
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17449_length_3458_cov_6.203644"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(84..749)
                     /locus_tag="PLT08_09030"
     CDS             complement(84..749)
                     /locus_tag="PLT08_09030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE04648.1"
                     /translation="MFKSKRRQIITPQSEHLKLVGTLAMLWGNDQFDTPPIERNSMVM
                     GMGLHDRGYGYLDNAPIGALRDEEWEGIARRTFTMECSDVIADTIVKYHFRRLAGYGS
                     SEIRKKLHADFTEGIHSQLERHNLSKALFDRTDRITDLLDTLSFEFCFDVPASGSVSV
                     FPRNGEETEVSIEYHVENGEINVHPWTFSVDEYRGYLIAYHAEGYPERLDPCILPYVL
                     KRK"
     gene            871..1287
                     /locus_tag="PLT08_09035"
     CDS             871..1287
                     /locus_tag="PLT08_09035"
                     /inference="COORDINATES: protein motif:HMM:NF016001.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family deazaflavin-dependent
                     oxidoreductase"
                     /protein_id="HNE04649.1"
                     /translation="MSEVNDWNKKIIAEFRANGGKVGGNFEGRPLLLLHHKGAKSGQE
                     RVNPVACVQDGERYVVIASKGGAPTNPDWYYNVVANPLTIVEVGTETLKVQATVAEEP
                     ERTRLYNKMAEIMPGFNEYREKTTRVIPVIVLTPVK"
     gene            1296..1802
                     /locus_tag="PLT08_09040"
     CDS             1296..1802
                     /locus_tag="PLT08_09040"
                     /inference="COORDINATES: protein motif:HMM:NF024698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNE04650.1"
                     /translation="MEAIHSERLTLRPLGLDDARFIFELVNEPSFLQNIGDRGVRTLA
                     DAEKYIENGPLASYAKNGFGLLAVTLKETGEPIGMCGLIKRDTLEDVDIGYAFLPKFW
                     LKGYAVEAARTVMGTAKALGLKRVVAIVDPANIGSIRVLERLGMSFEKMVRLTADDIE
                     LKLFSVAL"
     gene            complement(1857..2795)
                     /locus_tag="PLT08_09045"
     CDS             complement(1857..2795)
                     /locus_tag="PLT08_09045"
                     /inference="COORDINATES: protein motif:HMM:NF021221.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4870 domain-containing protein"
                     /protein_id="HNE04651.1"
                     /translation="MENPTSDEKILAALAHASIILMFLGPLGAVIIWAIQRTKSKYVR
                     YHALQAMGYQTFSFWIWMFMMFFFMIILFGFGAIIGVLSSKSPSAMPPDFLFFIQPLF
                     MLVIFGMWGIIFLVGFIGAVFCGLGKEFRYPFIGSWLQTKILNAPTEEEAEKWEDLWV
                     ASICHTTAILMMWGMIMPIIVWFSQKDRSARLKFQSMQAFLYQLIVTVAYFISYAGFF
                     FVYVFGIAIFAASGVMADPSQTTMPAGFEVGFIIIMAIFMLFWLAWMILYPAYLITAG
                     VAAVRTLRGHDFKYPLLGKFLAKRMSPPESHEVIQA"
     gene            2984..3388
                     /locus_tag="PLT08_09050"
     CDS             2984..3388
                     /locus_tag="PLT08_09050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE04652.1"
                     /translation="MNYRLLATINAVLLAVVGLIFLVVPVLALDFFNTETYTATSLVA
                     RFLGSAMLLAGMFIWVAKDLTDAGTVRTMTIMLLVSSLVSFILTLVGMVGVDVIRANG
                     WIPLVVQILFVLGYGYLVSGVTITATVQPKKK"
BASE COUNT          926 a          737 c          918 g          877 t
ORIGIN      
        1 aataagatcg tttcggctga gtttatctcg agggcaaaat agaacttaat gaaaaggtct
       61 cgcttgaaat agcgagacct tttttatttt ctcttgagta cataaggaag gatacatgga
      121 tcgagccgtt cggggtagcc ctcagcgtga taggcgatga gataaccgcg atattcatcc
      181 acagaaaatg tccacgggtg gacgttgatc tcgccgtttt caacatgata ttcaatgctt
      241 acttctgtct cttcgccgtt gcgcggaaag actgatacgc tgccagaggc aggaacatca
      301 aagcagaatt caaaagagag cgtatccaaa agatctgtga tgcggtcggt gcggtcgaag
      361 agagctttcg agaggttgtg tctctctaat tgagagtgga tgccctctgt aaaatcggca
      421 tgtaattttt tgcgaatctc agaactgcca tacccggcga gacgtcgaaa atgatatttg
      481 acgatggtat cggcgatcac gtctgaacat tccatggtga aggttcggcg ggcgatgcct
      541 tcccattcct cgtcgcgtag agcgccaata ggagcattat caagataacc atacccgcgg
      601 tcatgcagac ccatgcccat caccatggag ttgcgttcga tgggtggcgt atcgaattgg
      661 tcatttcccc ataacattgc caatgtgcca accagtttga gatgttcaga ttgcggggtg
      721 atgatttgtc tgcgttttga tttgaacata ctacatctcc aaaatttgac tataaagttt
      781 catatgcgat tctatcaata aataattctc ttagagtaag agaaaataaa tagagaccat
      841 cgaaacataa aacaccatag gagaattttc atgagcgaag taaacgattg gaacaagaaa
      901 attattgccg agtttcgcgc caatggcggg aaagtgggcg gaaactttga aggcagacct
      961 ttgttgctct tgcaccacaa gggcgccaag agcggacaag aacgggtgaa cccggttgcc
     1021 tgtgtacaag atggcgaacg ttatgttgtg atcgcttcaa aaggcggcgc accgaccaac
     1081 cccgattggt actataacgt ggttgccaac cccttaacca tcgtggaagt gggaacagaa
     1141 accttgaaag ttcaagctac cgtagcagaa gaacccgagc gcacccgcct ctataacaaa
     1201 atggcagaga taatgcccgg tttcaacgaa taccgtgaaa agacgacacg ggttattcct
     1261 gtgatcgtgt tgacaccagt gaagtaaatt ttctgatgga agcaatccat tcggaaaggc
     1321 ttacgttgcg tccactgggc ttggacgatg ctaggtttat ttttgagctg gtgaatgaac
     1381 cttcttttct tcaaaacatc ggtgaccgtg gtgtgcgaac tttggcggat gcagaaaaat
     1441 acatcgagaa cggtccgctt gccagctatg ccaagaacgg attcggtttg ttggctgtta
     1501 cgctgaaaga gacgggggag ccgattggca tgtgtggctt gatcaagcgc gataccttgg
     1561 aggatgtgga tatcggctat gcctttttgc cgaagttttg gttgaaaggg tatgctgttg
     1621 aagcggcacg gactgtgatg ggcacagcaa aagccctggg attgaaacga gtggttgcga
     1681 ttgtggaccc tgccaacata ggttcgatcc gcgtattgga gaggctggga atgtcattcg
     1741 agaagatggt tcgcttaacg gcggatgata tcgagttgaa gttgttttcc gtcgctttat
     1801 aaaataaaac ggactcaggc gaaatgtctg agcccgtttt tgattcatgg atgggtttag
     1861 gcttggatca cttcatgcga ctcaggggga gacattcgtt tggcaaggaa tttgcccaag
     1921 agtggatatt tgaaatcgtg cccgcgcaaa gtgcgcacag cggcaacccc agcggtgatg
     1981 aggtaggctg ggtagaggat catccatgcg agccagaata gcataaagat agccatgata
     2041 atgatgaaac caacctcaaa cccggcaggc atagtggttt gagaggggtc tgccatcaca
     2101 ccgctggctg cgaaaatagc aattccaaaa acatatacaa agaagaaccc ggcatacgag
     2161 atgaaatatg ccacagttac gatgagttga tataggaacg cttgcatcga ctggaacttg
     2221 aggcgggcgg agcggtcttt ttgtgagaac cagacgatga tcggcatgat catgccccac
     2281 atcatcaaaa tggcggtggt gtgacaaatg ctcgcgaccc agagatcttc ccacttttcg
     2341 gcttcttctt cggtgggggc gttgaggatc ttggtttgca gccaagaccc gataaatgga
     2401 tagcggaact ctttacccag cccgcaaaag acggcgccaa tgaaccccac tagaaaaatg
     2461 atgccccaca tgccaaagat caccagcatg aacaaaggtt gaataaaaaa gagaaaatcg
     2521 ggcggcatgg cggatggaga tttgctgctc aatacgccga tgatggcgcc aaacccaaat
     2581 aggatgatca taaaaaagaa catcatgaac atccagatcc aaaagctaaa ggtttggtag
     2641 cccatggctt gcagcgcatg gtagcggacg tattttgatt tggtgcgttg aattgcccag
     2701 atgatgaccg ccccgagcgg acctagaaac atgaggatga tcgaagcgtg cgccagagcg
     2761 gcaaggattt tttcatcaga tgtggggttt tccatgggtt tcctcctgcg ttgaattgac
     2821 ttgatgatac ctctattctc aaaaaataaa atgtgacatt gggattaaat ttgacgccat
     2881 gcaagggaaa tgtaaggttt gcgcttcaaa attcctttgc gctgttttcg gagcgttgta
     2941 tagtagcttt gttctacatt cacaccgact taaggagaat acaatgaatt accgactttt
     3001 agcgaccatc aatgccgttc ttctagctgt agtagggctt atatttttgg tcgtgcccgt
     3061 gctggcgctt gatttcttta ataccgagac ctacacagcc accagtttgg tagcccggtt
     3121 tttaggctct gccatgcttc tggcagggat gttcatttgg gtcgccaaag acctgaccga
     3181 tgcaggcacc gtccgcacga tgacgattat gttgctggtt agctcccttg tgagctttat
     3241 cttgaccctg gttggcatgg tgggcgtgga cgtgatccgc gcgaacggct ggattccgct
     3301 ggtggtgcag attctgttcg tgttgggcta tggctatttg gtttctggtg tgaccatcac
     3361 tgcaacggtt cagccaaaaa agaagtagat gccttgttaa gcaaaacggt cttgggtatt
     3421 tcacccaaga ccgttttttg tttaagcctg aaaacgaa
//
LOCUS       DAOQJE010000307         3454 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_17482_length_3454_cov_6.773757, whole genome shotgun sequence.
ACCESSION   DAOQJE010000307 DAOQJE010000000
VERSION     DAOQJE010000307.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3454)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3454)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3454
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17482_length_3454_cov_6.773757"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..3203)
                     /locus_tag="PLT08_09055"
     CDS             complement(<1..3203)
                     /locus_tag="PLT08_09055"
                     /inference="COORDINATES: protein motif:HMM:NF028237.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HNE04653.1"
                     /translation="MNFIYTPYSIPLIAAAGISIFVAIYAWRHRSSPGALALTLLAAA
                     IAQWILGYILEISSADLNTIYIWGVSQYIGIAFAPYAWLIFAIDFAGYSKLLTRRFYV
                     LTAIFPVITVLLAWTTRWHGLVWAKYDISLVEGFSALATQKGYWFYAHTAYSYLMLLI
                     GTILLVRALVRLQGLYRSQVIAMLIAVFAPWIGNVLYLTGNSPIPYLDLTPFSFTVTV
                     AALAWAIFGFHLVDITPIARDLVIDSLQEGVIVLDANDRVADINSSAARIFGIQISHA
                     IGTLASELFTPWQSLRERLREDEDWVQEFSNGVGQAERQYEAAISHIQDAQGRNAGRV
                     MIVREVLSSSTRRRPASSSTEAASVSDSVDTTIETPKWLDAYPWLKKIFVYFYTPINT
                     DLVVPPNISPTWYLARERSFTIILRVGALAGTIAYFFTLPVMRVASRFSISSLFFGIV
                     IVLLWFLGGGRKIGFETRANVFLVLIYFMSVNETIGYGFSVECFLFFTAFVTISTLLT
                     GRRGLRLATLTSFITLVLFAWLIGGGYYIPLNLTATTLRPQSLAIGFTNLFVFLATSS
                     AIANATIVLMENLNSAWRKESQTSNLLQQERDLLEQRVEERTHDLQEAESKFRTLVEQ
                     LPAVLYRDDADQNGKNNYYSPQAERMLGYPMSVWEKDSMLWHSILHPDDKERAIETIN
                     ETLSNGHSLSEYRLFDSDGRVVWVRDEAILVRESSGKPLFVQGIMQDITEIKKAEEEI
                     RKLSRAIEQSGNSIIITDTNGDIEYVNPTFEKITGYSFNEIKGQNPRILKSGRQRAEF
                     YDKLWATISSGNVWHGVFHNKRKDGTLYWENATIAPVMDATGRVTNYVAIKEDITARR
                     EADEQLRKLSQAVEQSANTVIIMDREGVIEYVNPTFSLITGYSSEDALGKTPERLLNP
                     ASKLDNFRNQEWWMTVNAGNTWRGEFRNYRKDGQVFWESASIAPVMDSNGAVTNFIEI
                     KQDVTEQRKLQDQLQKRNEYLSILHQVTLDLLNRRDVEDLLQVIVDRASVLLDATFSE
                     LMLEEDGYLVVKAFTANQPNLKGDRVGREQAKLSWQA"
BASE COUNT         1028 a          824 c          748 g          854 t
ORIGIN      
        1 aaagcctgcc acgaaagttt tgcctgctca cgcccaaccc ggtcgccctt taagtttggt
       61 tggttggcgg taaaagcttt caccaccaaa tacccatcct cttccagcat gagctcgcta
      121 aacgttgcat ccagcagaac agatgcgcgg tctacgatca cttggagcag atcttccaca
      181 tcacgacggt tcaaaagatc caaggtaact tggtgcaaaa ttgataaata ttcattccgc
      241 ttctgcaatt ggtcttgcag ctttcgctgt tcagtaacgt cttgtttgat ctcaataaag
      301 ttggtcacag cgccattcga atccatcacg ggggcaatac tggctgattc ccaaaatacc
      361 tgcccatctt tgcgatagtt gcggaactca cctcgccaag tattcccagc attgaccgtc
      421 atccaccact cttggtttcg gaaattatca agtttcgagg ctggattcag cagtctctca
      481 ggggttttgc ctaaggcatc ttcagaagag tatcctgtga tcagtgagaa agtggggttc
      541 acatattcta taacaccttc acggtccatg atgatgaccg tattagcact ctgttctact
      601 gcctgtgaca acttgcgcaa ctgctcatcc gcttctcttc gagcagtaat atcttcttta
      661 attgccacat aatttgtcac cctacccgtt gcatccataa caggcgcaat ggtggcattc
      721 tcccaataca gggttccatc cttgcgtttg ttatgaaaca ctccatgcca aacatttcca
      781 gaggaaattg ttgcccacaa cttgtcataa aattctgcac gttgtctacc agatttcaag
      841 atcctggggt tttgcccctt gatttcatta aaagaatagc ctgttatttt ttcaaacgtg
      901 gggttcacat attcaatatc gccattcgta tcggtaatga tgatcgagtt tccgctttgc
      961 tcaatagcac gtgataattt tcgaatctct tcttctgcct ttttgatctc cgtaatatcc
     1021 tgcataatgc cttgaacaaa caaaggcttt ccagacgatt cacgcaccaa gatggcttca
     1081 tcacgaaccc aaacaacccg accatcactg tcaaacagac gatactccga gagggaatgt
     1141 ccattagaga gcgtctcatt aatagtctca atggcacgtt ctttatcgtc agggtgaaga
     1201 atgctatgcc aaagcatgga atccttctcc caaacagaca tggggtaccc tagcattctc
     1261 tcggcttgtg ggctgtagta gttgttcttt ccgttttgat ctgcatcgtc gcggtacaaa
     1321 actgcaggta actgttcaac aagggttcgg aatttagatt ctgcctcttg caaatcatgt
     1381 gttcgttctt ccacgcgctg ctcaagaagg tcacgttcct gttgcaataa atttgaagtt
     1441 tgtgattcct tacgccaggc agagtttagg ttctccatca agacgatggt ggcgtttgca
     1501 attgcagaag aagttgccaa aaacacgaat aaattcgtaa acccgatggc aagcgattgc
     1561 gggcggagtg ttgttgccgt tagattcagg ggaatatagt aaccgccacc aataagccag
     1621 gcgaataata caagcgtgat aaaactggtc agggttgcaa gccgaagtcc acgccgcccg
     1681 gttagaagag tagaaatcgt tacaaaagca gtaaagaata aaaaacattc cactgagaaa
     1741 ccatacccaa tggtttcatt aaccgacata aaatagatta gaactaaaaa gacatttgca
     1801 cgtgtttcaa agccgatctt tcgcccgccc ccaagaaacc ataaaaggac aataacaatt
     1861 ccaaagaaaa ggctggatat tgaaaaacgc gaagcaacac gcatgacagg cagagtaaaa
     1921 aagtatgcga tggtccctgc cagtgcacct actcgcaaga taatggtaaa agaacgctca
     1981 cgtgccaaat accatgtggg gctaatatta ggcggcacta ccaagtctgt attgataggc
     2041 gtataaaaat agacaaaaat tttctttagc catggatatg catccaacca cttcggagtt
     2101 tcaatcgtag tatccacgct atcagaaaca gaagcagcct cggtggaaga tgaggcgggt
     2161 cttcgtcgcg tagagctaga aagcacctca cggacaatca tcaccctgcc agcatttctc
     2221 ccctgtgcat cctggatatg agagatcgcc gcttcatact gacgttcagc ctgtccaact
     2281 ccatttgaaa attcctgcac ccaatcttca tcttcgcgca aacgctcgcg aagtgactgc
     2341 cagggtgtaa acaactcaga tgccaatgtt ccaatggcgt gagaaatttg gataccaaaa
     2401 atacgagccg cagacgagtt aatatctgca actctatcat tggcgtccag tacaatcaca
     2461 ccttcttgaa gggaatcgat aaccaaatct ctagcaatgg gcgtgatatc aacaagatga
     2521 aatccaaaga ttgcccaagc caatgccgca acggtgacag tgaaagaaaa aggcgttaga
     2581 tcgagatatg gaatagggct gttgcccgtt agatacaaaa cattcccgat ccatggcgca
     2641 aagactgcaa ttagcatcgc tataacttgg cttcgataca aaccttgtaa gcggaccaaa
     2701 gcgcgcacca acaagatcgt tccaatcaga agcatcaagt aggaataagc agtatgggcg
     2761 tagaaccaat aacctttttg agtggcgagc gcagaaaaac cttcaacaag gctaatgtcg
     2821 tatttcgccc aaaccaaacc atgccagcga gtcgtccatg ccaaaaggac ggtgatgact
     2881 gggaagatcg ctgttagaac ataaaaacgt cgtgtaagaa gttttgaata gccggcaaaa
     2941 tcgatcgcaa atattagcca tgcgtatggt gcaaaggcaa ttccaatgta ctgacttacc
     3001 ccccaaatgt aaatggtatt caaatctgca ctggatattt ccaaaatata tccaagaatc
     3061 cactgagcaa tagctgctgc aagaagggtc aacgccaggg ctccgggaga agaacgatgc
     3121 ctccaggcat agatggctac aaaaatagaa atcccagccg ctgcgattaa aggtatggaa
     3181 taaggcgtgt aaatgaaatt catgggcgat tcctttggca aaatcagccc aattatatag
     3241 tcatcccctg ctgttggttc attacaaagc tattaggttt tagcccttac cggagccaga
     3301 aggtaagagt taggaaatac gtggtcagcc caaagacggc aagcgctgtg gaagtgagaa
     3361 gcctccaatt tggcgggttt ccattcgcaa cagaccgaag ttggaagacc tgaaaaatgg
     3421 caaaaggcaa ggtgagaaat gctggcgcaa ccaa
//
LOCUS       DAOQJE010000308         3454 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_17484_length_3454_cov_4.744631, whole genome shotgun sequence.
ACCESSION   DAOQJE010000308 DAOQJE010000000
VERSION     DAOQJE010000308.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3454)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3454)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3454
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17484_length_3454_cov_4.744631"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..334)
                     /locus_tag="PLT08_09060"
     CDS             complement(<1..334)
                     /locus_tag="PLT08_09060"
                     /inference="COORDINATES: protein motif:HMM:NF013982.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF47 family protein"
                     /protein_id="HNE04654.1"
                     /translation="MFRIFRRKEDKFQKLIEQQAEISFEGLKLLVKYLETSAPDVAEE
                     LSFKEKEADEVRRILIDELNRTFVTPFDREDIFSLSRSIDDVVDYADTTVVEMVILKV
                     SPTPYMQRI"
     gene            complement(360..1796)
                     /locus_tag="PLT08_09065"
     CDS             complement(360..1796)
                     /locus_tag="PLT08_09065"
                     /inference="COORDINATES: protein motif:HMM:NF018012.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0015948 - methanogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trimethylamine methyltransferase family protein"
                     /protein_id="HNE04655.1"
                     /translation="MSFAALLTQEQVEKIHDASLEILEDVGLKVRYEPARELFKKHGC
                     MVEDERVKFPRAVVEKYRKLVPPSFTFHARDPKFDKTIPQDSPVIVTASSAPDIIDPV
                     TGKERRAESRDIAQIAHLINELPGYDIFSISTLADDAPADQFTVSRLYPSIKYCLKPI
                     RVTTTNMKDTMSIMEMAYIVAGGEAAYKEHPFLTHHYCPVVSPLTMDHLSTENVMFFA
                     QQGLPVYPTIVPNAGLTSPMSMAGSLVQGNAEFLSALMLMQMTKEGTPTIYATLGTVA
                     DMRSGAYTSGAIECGMLHMAFAQMARFYDVPAGGYVGLTNSKLNDAQAGYETGMSGIA
                     GVLGGMDMFNIGGLIDALKTFDFAKAVIDDEIAQMMKRMKRGVSFSDEDLGVNLIKEI
                     GPGGSFITAKHTTSRMKTEAVMTKIADRDARSIWEKKGATDTHAKALKKAKDVMASNT
                     TPLISPEVDEKIRAAFPGLVPGMLEPIP"
     gene            complement(1820..2911)
                     /locus_tag="PLT08_09070"
     CDS             complement(1820..2911)
                     /locus_tag="PLT08_09070"
                     /inference="COORDINATES: protein motif:HMM:NF012515.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HNE04656.1"
                     /translation="MAKKNSSPAYIGFGMLTPVYIMSLDQLPKLNTGAIVHQVSDYVY
                     DDAAIIACNLSQWNVPSAMIGTAVGKDLLGKYVADKLKELGVKGEVRFTTKYKTPLEV
                     NVSDKKGARTYFWQRSKEILDTLSTADLSLIKSAKLLYVDWYDGEHIVRAMAEAKKHN
                     VPVFLNFEHGHQNPDLLKKYAGMVTVCQAVTDAAQIGKRQAMLGTARKLIKSGIQTAI
                     ITMASQGCMVVQGDEIIRAYAPKVKAVDASGAGATFSSGYIYGYLNGWSLEDTVRFAI
                     AAASLKVTRSGLDMFPVSEIKGLARMVRIERMVYRGDQFRTIRKLLTLPENSPLVNNA
                     LVKESQKLAERILPKKKIERKKIKRSLVE"
     gene            complement(2930..>3454)
                     /locus_tag="PLT08_09075"
     CDS             complement(2930..>3454)
                     /locus_tag="PLT08_09075"
                     /inference="COORDINATES: protein motif:HMM:NF020253.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage T C-terminal barrel
                     domain-containing protein"
                     /protein_id="HNE04657.1"
                     /translation="YIPNNRATMIWDMLVDAGKEFGMELGGYKVLDPLRLEKGFKYFT
                     TDITPTTTPYEAGLGFCVDLNKGDFIGRDALVKQKADGVTRKLCTLVLTDTDEFVQIY
                     GGEAVHHEGKVVTRIRSGGYGFTVKKNILYAYLPVDLAVKGTRFTIELIEGNLAAEVT
                     ANVLYDPKSEKLKA"
BASE COUNT          895 a          887 c          741 g          931 t
ORIGIN      
        1 caattcgctg catataaggt gttgggctga cttttagaat caccatttct acaacagttg
       61 tatcggcata atcaaccaca tcatcgatcg aacgagagag cgaaaagata tcttcacggt
      121 caaacggtgt gacaaatgtg cggttcagtt catcgatcaa gatgcgacga acttcatccg
      181 cctctttctc tttgaacgac agttcttcag ctacatctgg agccgacgtt tcaagatatt
      241 tcaccaataa cttcaagcct tcaaacgata tttccgcttg ttgctcaatc agtttttgga
      301 atttatcttc cttgcgccta aaaatacgaa acattcaaat ccttctttta ttccttgtat
      361 tacgggatag gctctaacat cccgggcact aagccaggga atgcagcgcg aatcttttca
      421 tccacttcgg gcgagatcag aggagtggta ttggaagcca taacatcttt ggctttcttc
      481 aacgccttgg catgtgtatc cgtagcgcct ttcttctccc aaatggaacg agcatcacgg
      541 tcagcgattt tggtcatcac cgcttcggtc ttcatgcggc tggtagtgtg tttggctgtg
      601 ataaaggagc cgccaggtcc gatctctttg atgagattga ctcccaaatc ttcatcgcta
      661 aagctgactc cacgtttcat acgcttcatc atctgtgcaa tttcatcatc aatcactgct
      721 ttggcaaaat cgaaggtctt caatgcatcg atcaaaccgc caatgttgaa catatccata
      781 ccgccgagca ctcccgcaat gccagacata ccggtttcat accctgcctg agcatcgttc
      841 agtttggagt tcgtcaagcc aacatatccg cccgcaggca catcataaaa acgcgccatc
      901 tgagcaaatg ccatatgcaa catgccgcat tcaatggcac cggaggtata tgcacccgaa
      961 cgcatgtcag ccactgtgcc cagtgttgca tagatcgtcg gtgtgccttc ctttgtcatc
     1021 tgcatcaaca tcagcgcaga taaaaattca gcattacctt gaaccaaact accagccata
     1081 gacatgggcg atgtaagacc ggcattcggc acaatggttg ggtaaacagg cagcccttgt
     1141 tgtgcaaaga acatcacatt ctccgtcgag agatggtcca ttgtcaacgg cgaaacgacc
     1201 gggcaatagt gatgcgtaag gaacggatgc tccttatatg ccgcttcccc accagccaca
     1261 atgtatgcca tttccatgat gctcatggtg tctttcatgt tggtcgtagt cacacggatc
     1321 ggtttaagac aatacttgat ggacggatac aagcgcgata ccgtgaattg atccgcgggt
     1381 gcgtcatctg ccaacgtgga aatggagaag atatcgtatc cgggcaattc gttgatgagg
     1441 tgggcgatct gcgctatatc tctggattcg gcgcgtcttt ctttgccggt cactggatca
     1501 ataatatcgg gcgcggaact cgctgtaaca atgaccgggc tatcctgcgg gatggtctta
     1561 tcaaatttcg ggtcacgcgc gtgaaacgta aaagagggcg gaaccagttt gcgatacttt
     1621 tccacaacag cgcgcgggaa tttcacacgc tcatcttcga ccatacagcc atgtttcttg
     1681 aacaactcgc gagcaggttc atagcggacc tttaagccaa catcttccaa aatctctaac
     1741 gaagcatcgt gaatcttttc gacttgttct tgcgtaagca aagcagcaaa tgacatggtt
     1801 cacatcctta taaaataaat tattcaacca gtgagcgttt gatcttttta cgctctattt
     1861 tcttcttagg caaaattctt tccgccagct tctgactttc tttcacgagc gcattgttaa
     1921 ccaatggact gttctctgga agggtcaaca acttacgaat agtacggaat tggtctccgc
     1981 gatacaccat acgttcaata cgaaccatgc gagccaatcc tttgatttca gatactggga
     2041 acatatccaa cccagaacgg gtcactttta aagacgctgc agcaattgca aaacgaaccg
     2101 tgtcttccaa gctccagcca ttgagataac cataaatata gccagatgaa aatgtggcgc
     2161 ccgctcctga ggcgtcaaca gccttaacct tgggcgcata ggcgcggata atttcatcac
     2221 cttgcaccac catacacccc tgacttgcca tagtgatgat agctgtttga ataccagatt
     2281 taatgagctt gcgcgctgtg cctaacatag cttgacgttt gccaatctgc gccgcatcgg
     2341 tcactgcctg acagaccgtg accatgcccg catatttttt gagcaggtct gggttctgat
     2401 gcccatgctc aaagttcaaa aagaccggca cattatgttt cttggcttct gccatggcac
     2461 ggacgatatg ctcgccatcg taccagtcca catataatag ttttgcagat ttgatcagtg
     2521 acaaatccgc tgtggacaat gtatcgagaa tttcctttga acgttgccaa aaatacgtgc
     2581 gcgcgccttt tttgtcagag acgttcactt ccaaaggcgt tttatatttc gtcgtaaagc
     2641 gcacttcccc ttttacgccc aattctttca atttatctgc gacgtatttt ccaagcaagt
     2701 ctttacccac cgctgttcca atcatggcgg acggtacatt ccactggcta aggttgcaag
     2761 caatgatggc agcatcgtca tacacataat cgctgacctg atgcacgatg gcacctgtat
     2821 ttaacttggg caattgatcc agcgacataa tgtacacagg cgtcaacatg ccaaagccaa
     2881 tgtaagctgg agaggaattt ttcttagcca taattagagg gtaatcaatt tacgctttca
     2941 gcttctcact cttcgggtcg tacaagacat tggcagtcac ttcagcagcg agattgccct
     3001 cgatcaattc aatggtgaac cgagtgcctt taactgccaa atccactggc aggtaggcat
     3061 acaaaatatt ctttttcacg gtaaagccgt agccgccact gcggatacga gtgacaactt
     3121 tcccttcgtg atgaaccgct tcaccgccgt agatctgcac aaattcatcg gtatcggtca
     3181 ataccaaagt acagagtttc cgcgttactc catctgcttt ttgcttgacc aacgcatcgc
     3241 gcccgatgaa gtcacccttg ttcaagtcca cgcaaaagcc aagaccagct tcatagggtg
     3301 tggttgttgg ggtaatatcg gtcgtgaaat acttaaagcc tttttccaaa cgtagcgggt
     3361 ccagcacttt atagccgccg agttccatgc caaattcttt accagcatca acaagcatat
     3421 cccatatcat ggtggcgcga ttgttcggaa tgta
//
LOCUS       DAOQJE010000309         3453 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_17493_length_3453_cov_6.121248, whole genome shotgun sequence.
ACCESSION   DAOQJE010000309 DAOQJE010000000
VERSION     DAOQJE010000309.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3453)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3453)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3453
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17493_length_3453_cov_6.121248"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..230
                     /locus_tag="PLT08_09080"
     CDS             <1..230
                     /locus_tag="PLT08_09080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HNE04658.1"
                     /translation="YMITRQGLRVYRDALDVRNDPRGKPYYWIGGEAPTGVDEPGTDF
                     GALKAGFVSITPLQLDLTHLKAMDVLKKWKF"
     gene            242..1513
                     /locus_tag="PLT08_09085"
     CDS             242..1513
                     /locus_tag="PLT08_09085"
                     /inference="COORDINATES: protein motif:HMM:NF024298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE04659.1"
                     /translation="MNSAKGRSRKGNFSLLILIIFIVGCSSKAETENPQKATNSPTPV
                     SSPTAVLYTSTPSATPQATPIIEVIDKCISVEDTLPSDLQLTGTWVRQEPDPYLEIIE
                     ENTKYRVPLDGHGRLYDSEGSWSVSPNGEWLAYIDTVYDTTGRFWRTNGGLLKVIHSS
                     GYSLSMDYWPMSNQKIRGWVDDENLILELGQHSLVLNPFSGKWYELKDPDWLSSLIVN
                     KPWWYEIKKYNPHLDSVIVRLDNHSEIKEAVSGNTLFGDTALGFFGESVWSSDAVMLA
                     FATNDGNVIHIFQNNKEILTLDFLNDKSLNSNFREGISISELKWSLNDQRLLIGTYDD
                     NILLLDINEQILYKLCFENKEINNYAWDNFFYLKSGQHIVAPVNLRSQDYHYEQFDVL
                     IDTTTMRAYRLPTSDLNGRIGWLALPESAEK"
     gene            1515..1796
                     /locus_tag="PLT08_09090"
     CDS             1515..1796
                     /locus_tag="PLT08_09090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE04660.1"
                     /translation="MNFLKKLFALREPYKKEYYTFNVKCKRCGETITGRVDLDNDLSI
                     EYEDGGDVFYARKVLMGENKCFQRIEVNLKFNADRQVIEKDIIGGDFIE"
     gene            1793..2251
                     /locus_tag="PLT08_09095"
     CDS             1793..2251
                     /locus_tag="PLT08_09095"
                     /inference="COORDINATES: protein motif:HMM:NF016178.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ASCH domain-containing protein"
                     /protein_id="HNE04661.1"
                     /translation="MTDSQIETYWEAFLAAHPEYRGKPYLAEPFGDNPALADELGNLV
                     LSGLKSATCSAVWNYEAEGQPFPQVGTLWLVLSGRNEPMCVVETVEVTFRKYNEVDAD
                     FARAEGEDDLSLESWRAGHKRFFTRMLATIGKEFVEDMPLVCERFRVIYK"
     gene            2264..2959
                     /locus_tag="PLT08_09100"
     CDS             2264..2959
                     /locus_tag="PLT08_09100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE04662.1"
                     /translation="MKRSPNFLLACVLSFSMIACGRGILPQQSSVIAEAPDTPKPIEK
                     TLSSFTPIIGTDYLMAGIVPIEVTRDSTLNPFEWISSSGYSSYTSYATYNYVFFNTQT
                     EEYHRLLPDNNSVILQTAGFPQQVYDPNDPQKPAPVIEFWMFNIIKADTNGDTYFDYR
                     DKLTIGISDVSGSGYTELIQNVDVIQSQYYKDPSNFFIIYNVNDKNFIAKVNPITRQI
                     LSTTEMDLGEDVK"
     gene            2949..>3453
                     /locus_tag="PLT08_09105"
     CDS             2949..>3453
                     /locus_tag="PLT08_09105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE04663.1"
                     /translation="MSNNLQRITTVVLILLAALLIAQAAFSLQWPIAHDEAPLFYEAF
                     LMQNGQVPYKDFFDFQMPGSFFAYYILGILSNFGPLRIRILDLIILVAISTITYFAMQ
                     KRFGTSAALAAPILFALKYMQGGPNLSLQREYIVLIFISLSIWIGVTDSLTFRKRLSL
                     GLLYGLAA"
BASE COUNT          979 a          863 c          705 g          906 t
ORIGIN      
        1 gatacatgat cacgagacaa ggcttgcgtg tctatcgcga tgcgctcgat gtgcgcaatg
       61 acccgcgcgg taagccctat tactggatcg gcggcgaagc ccccactggc gtggatgaac
      121 ccggcacaga ctttggcgca ctgaaggctg gttttgtttc catcacgccc ctgcaattgg
      181 acttgaccca tctcaaggcg atggatgtgt tgaaaaagtg gaagttttaa atagaaataa
      241 tatgaatagt gcaaaaggga gatcaagaaa aggtaacttc tcattattga ttttgataat
      301 ttttattgtt ggctgttctt ccaaagctga aacagaaaat ccgcaaaagg cgaccaattc
      361 tcctacaccc gtctcatcac caactgccgt tttatataca tcaacgcctt ctgccacacc
      421 gcaagcgact ccaataatcg aagttataga taaatgcata tcagttgaag acaccttgcc
      481 atctgacctc caactaactg gtacatgggt aagacaagaa ccagaccctt accttgaaat
      541 tatcgaagaa aatacaaaat atagagttcc tcttgatggc cacggacggc tttatgactc
      601 tgaaggttct tggtcagttt caccaaacgg agaatggctc gcatatatag acacagttta
      661 tgacactaca ggaagatttt ggagaacaaa tggtggctta ttaaaggtca ttcattcatc
      721 aggatattcg ttgtcaatgg actattggcc catgagcaat caaaagattc ggggttgggt
      781 tgatgatgaa aaccttattc tcgaattagg tcaacactca ctggtcttaa atccgttttc
      841 aggaaaatgg tatgaattaa aagacccgga ttggctaagc agtttaatag ttaataaacc
      901 ttggtggtat gaaatcaaga aatacaatcc gcatttggat agtgtcatag tgaggttgga
      961 taatcattct gagattaaag aggctgtttc aggaaacact ttgtttggcg atactgcact
     1021 tggctttttt ggtgagagtg tttggtcgtc tgatgctgtt atgctagcat ttgcaactaa
     1081 tgacggaaat gtaattcaca tatttcaaaa taacaaagag atattgaccc tcgacttttt
     1141 gaacgacaaa tcattaaatt caaatttcag agaagggata tccattagtg aactaaaatg
     1201 gtcattaaat gatcagaggc tacttatagg cacctatgac gacaatatcc ttttattaga
     1261 tattaatgaa caaatattgt ataaactttg ttttgaaaac aaggaaatta ataattatgc
     1321 atgggacaac ttcttttatt taaaaagtgg acagcatatc gttgctccgg tgaatttgag
     1381 aagtcaagac tatcactacg agcaattcga tgtgctcata gatacaacga caatgcgggc
     1441 atacaggtta cctacatctg acttaaatgg tcgcatcggc tggcttgcat tacccgagtc
     1501 agcggagaaa taaaatgaat ttcctcaaaa aacttttcgc tctccgtgaa ccctataaaa
     1561 aagaatatta cacttttaac gtcaaatgca aacgctgcgg tgagacgatt actgggcgcg
     1621 tggacctcga caacgacctc agcattgagt acgaagatgg cggcgatgtt ttctatgctc
     1681 gcaaagtatt aatgggcgag aacaaatgct ttcagcgtat agaagtaaac ctcaagttca
     1741 atgcagatcg ccaagtgatc gaaaaagaca tcatcggcgg agatttcatc gagtgaccga
     1801 ctcgcaaatt gaaacctatt gggaagcgtt ccttgccgcc catcctgaat atcgcggcaa
     1861 accctacctt gccgaaccct ttggtgataa ccccgccctt gccgatgaac tcggcaacct
     1921 tgtcctcagt ggacttaaat ccgccacctg ttctgccgtg tggaattacg aagccgaagg
     1981 tcagccgttt ccacaagtgg gcacactatg gctcgtgctc agcggacgca atgaaccgat
     2041 gtgcgtggtt gaaactgtcg aagtgacctt tcgcaaatac aatgaagtgg atgcagattt
     2101 tgcccgtgcc gaaggcgaag atgatctttc tctggaaagc tggcgtgcag ggcacaaacg
     2161 cttcttcacc cgtatgcttg caacaatcgg aaaagaattt gtagaagata tgccgctcgt
     2221 ttgtgaacgc ttccgcgtca tttacaaata aagtgaggtc ctcatgaaac gctctccaaa
     2281 ttttctgctt gcatgtgtcc tatcgttctc catgatcgcc tgtggacgcg gcatcctacc
     2341 gcaacaatca tccgtcattg ccgaagcgcc agataccccc aaacccattg aaaagactct
     2401 ttcctcgttc actcccatta tcggcaccga ctatctcatg gcaggcattg tgcccattga
     2461 agtaacccgc gactccactc tcaatccctt tgagtggatc agcagtagcg gttactcaag
     2521 ttacacatcc tacgccacat ataactatgt tttcttcaac acccaaaccg aagaatatca
     2581 tcgcctcctg ccagataata actctgtcat cttgcaaact gccgggttcc ctcaacaagt
     2641 gtatgatccc aatgacccgc aaaagcccgc gcccgtgatc gaattttgga tgttcaacat
     2701 catcaaagcc gataccaatg gcgacaccta ctttgactat cgcgacaaac tcaccattgg
     2761 catttccgat gttagcggca gcggctatac cgaactaatc caaaatgtgg atgtgatcca
     2821 atcgcaatac tacaaagacc catccaactt cttcatcatc tataacgtca acgacaagaa
     2881 cttcatcgcc aaagtcaacc ccatcacccg ccagatcctc tccaccaccg aaatggacct
     2941 tggagaagat gtcaaataat cttcaacgca tcaccacagt cgttctgatc ctgcttgccg
     3001 ccttgctcat cgcacaggcg gcattctcgc ttcaatggcc catcgcgcac gacgaagccc
     3061 cgctcttcta cgaagccttc ctcatgcaaa acgggcaagt gccatacaaa gactttttcg
     3121 acttccaaat gcccggaagt tttttcgctt actacatcct cggcatcctt agcaacttcg
     3181 gtccgcttcg cattcgcatt ctcgatctca tcatccttgt cgccatctca accatcacat
     3241 acttcgcgat gcaaaaacgc ttcggcacga gcgcagccct tgccgcgccc attctcttcg
     3301 cgctcaaata tatgcagggc ggtcccaatc tttctctgca acgcgaatat atcgttctca
     3361 tttttatttc tctctcaatc tggattggcg tgaccgactc tctgaccttt cgcaaacgcc
     3421 tcagcctcgg tctactctac ggactcgccg cca
//
LOCUS       DAOQJE010000310         3448 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_17532_length_3448_cov_6.454170, whole genome shotgun sequence.
ACCESSION   DAOQJE010000310 DAOQJE010000000
VERSION     DAOQJE010000310.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3448)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3448)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3448
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17532_length_3448_cov_6.454170"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..871)
                     /locus_tag="PLT08_09110"
     CDS             complement(<1..871)
                     /locus_tag="PLT08_09110"
                     /inference="COORDINATES: protein motif:HMM:NF014344.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine deiminase family protein"
                     /protein_id="HNE04664.1"
                     /translation="MILDETHPLEEVMVWGEPGIEALLGQLLPQSRSLFRSYYEVPKA
                     RGEFACMSSLIESEGIKVTRAKDAVADLLKTREIPELPGSIQDLKRILHQKADSTFEH
                     YRLAKQSDLIRDGITHISPIDVYNAVKRDIDVVLEEDIAQYGEQGALRLNYLLSLSKP
                     WPMANIFYGRDQSQALTDRIILSSLRWNIRKPEVRIFKDALHHMGYGSVLADIREGTI
                     EGGDVAMFKGYCFIGVGARTNINAVKEVYRQIGGSLKEHRIELIAVVNKQHEEDSNFF
                     ISPTSEHMQVMHLD"
     gene            complement(937..2739)
                     /gene="argS"
                     /locus_tag="PLT08_09115"
     CDS             complement(937..2739)
                     /gene="argS"
                     /locus_tag="PLT08_09115"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR00456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HNE04665.1"
                     /translation="MFQKEQQLIEEKIKEYCAANYIALAELKWQPIPFSGEWGFATSF
                     FQTAANEAKLGKGTKAPVPQKAQEIAEQVKGQLGSVAGISHIEAVKGYLNVYFKTSEY
                     AHRVVEEVLKNGAEFGRGEAKPETVMVEYAQPNTHHSFHIGHARNTILGEVLARLVDF
                     AGYKTIRASYPGDLGLGVITVMWIYDKLYKGQEPVGVHERGQWLLKLYVEANQLLEKK
                     ENETPEQTAQREAYEAERREMYRKWDAGDEYVRELWRVTREWSLEELREVLHMLDVKM
                     DVWFYESEVDEPSKAIVEELIAKNIADDERPNGGAVIVKIDEKLGLTKEKYRTNVILR
                     RDGTTLYLTKDLALAKVKFEQYHVDRSIYVVDSRQALHLQQAFAILKLWGFKQSEKCY
                     HLGYGFVSLPEGAMSSRKGRVVLFKEVFDEAVKRVLSVNAEKSVEIAAEEREKVAAQI
                     GLGALVYSMLSVDNNKDIVFNIEEALSFDGRTGPYIQNAHVRATSILKKAGKVSEADF
                     NYDLTKQEIELIEQMSQFPQKVQQAAEEYRPLVMAMYAYDLANAFHSFYHAVPVLQSE
                     DEKVKNARLRLVAAAKQAIANALHLLDIQSPDVM"
     gene            complement(2814..3086)
                     /gene="rpsT"
                     /locus_tag="PLT08_09120"
     CDS             complement(2814..3086)
                     /gene="rpsT"
                     /locus_tag="PLT08_09120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HNE04666.1"
                     /translation="MANIQSQIKRNRQNEKRRLRNRNVRGAARTADNAARKVLTSDTP
                     ESKQAVMQAISALDKAAEKGVLHKNTVARRKSRLMKALAALTKKSA"
     gene            complement(3206..>3448)
                     /locus_tag="PLT08_09125"
     CDS             complement(3206..>3448)
                     /locus_tag="PLT08_09125"
                     /inference="COORDINATES: protein motif:HMM:NF013630.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM domain-containing protein"
                     /protein_id="HNE04667.1"
                     /translation="DSLGAIANAFNSTIDDIMKENNITDPNALFVGQVLVIPVNMVTP
                     TATRPPTSTPITPGPGTVLPTGTSTPINAAPATATP"
BASE COUNT          814 a          865 c          795 g          974 t
ORIGIN      
        1 tgtccaaatg catgacttgc atgtgttcac ttgtcgggct gatgaagaaa ttgctgtctt
       61 cttcatgctg tttgttgacg acagcgatta attcaatgcg atgctctttc aacgagccgc
      121 cgatctggcg atacacctct ttgaccgcgt ttatattcgt ccgtgcgccg acaccgataa
      181 agcaatagcc tttgaacatg gcgacgtcgc cgccttcaat tgtcccttcg cgaatgtctg
      241 ctagaacaga tccatagccc atatgatgta gcgcatcttt gaaaatgcgc acctcaggtt
      301 ttcggatatt ccagcgtaga gaagaaagaa tgatacggtc tgtgagggct tgcgattggt
      361 cacgtccgta aaagatattt gccataggcc agggtttgga gaggctcagt aaataattta
      421 ggcgtagagc accttgttcc ccatattgcg caatgtcttc ttcgagtaca acatcaatat
      481 cccgttttac tgcattgtat acatctatag gggagatgtg ggtgattccg tctcggatca
      541 agtcgctttg tttagcgagg cgatagtgct caaaggtaga atccgctttt tgatggagaa
      601 tccgtttcag gtcttgaata gagccgggta attcgggaat ttcacgcgtc ttcaataaat
      661 ctgctactgc atcttttgct cgcgtaacct taatcccttc gctttcgatc aaggaagaca
      721 tacaagcaaa ttcaccgcgc gctttgggta cttcataata actgcggaac aggctgcgcg
      781 attgcggtaa cagttgacca agcaaagcct caatgccggg ctcgccccaa accatgactt
      841 cttctaaagg atgtgtttca tcaagaatca tttatacctt cacttataaa aacagactaa
      901 atcctcaagg atttagtctg ttttttgtac tgatttttac atcacatctg gcgactgaat
      961 gtctaataga tgaagcgcat tggcaatggc ttgtttcgcc gctgccacca atcgcagacg
     1021 ggcattctta accttctcat cttctgattg caaaaccggc accgcatgat agaacgaatg
     1081 gaaggcgttc gccagatcgt aggcatacat cgccatcaca agcggacgat actcctctgc
     1141 cgcttgctgc accttctgcg ggaactgcga catttgttca atcaactcga tctcttgctt
     1201 ggtcaggtca taattgaagt ccgcttcaga aactttgcca gccttcttca aaatggaggt
     1261 tgctcgcaca tgcgcattct gaatatacgg acccgtgcgc ccatcaaaag agagcgcttc
     1321 ttcgatgttg aagacaatat ctttattgtt atccactgaa agcatggaat agaccaaagc
     1381 gcccaaacca atttgagccg caactttctc gcgttcttca gcggcaatct ccacgctctt
     1441 ttcggcattg accgagagca cgcgcttcac ggcttcatca aagacttcct tgaaaagcac
     1501 cacccgtccc ttgcgggaag acatggcacc ctcgggcagg ctcacgaaac catagccgag
     1561 gtgatagcat ttttccgatt gcttgaaacc ccataacttg agaatggcaa atgcctgttg
     1621 caaatgcaat gcctggcgtg aatctacaac atagatcgag cgatctacat gatattgttc
     1681 gaacttcact ttggctaatg ccaaatcttt ggtcaggtaa agggttgtac cgtcgcggcg
     1741 caaaatgacg ttcgtgcgat acttttcttt ggtcaaccca agtttctcgt cgatcttcac
     1801 gattactgcg ccgccgttcg gacgttcatc atctgcaata tttttagcaa tcaactcttc
     1861 aacgattgct ttcgagggtt cgtccacttc agactcgtag aaccacacat ccatctttac
     1921 atcaagcatg tgcagcactt cgcgcagttc ttccaaactc cactcgcggg tcacacgcca
     1981 taactcgcgc acatattcat cgcctgcatc ccacttgcga tacatctcgc ggcgttcggc
     2041 ttcgtaggct tcgcgctgag cggtctgctc aggcgtttcg ttctctttct tttctaacaa
     2101 ctgattggct tccacgtata acttcaagag ccactgtccg cgctcgtgca cacctacggg
     2161 ttcttgtccc ttatataatt tgtcgtagat ccacatcaca gtgatgacgc caagtcccaa
     2221 gtcaccgggg tatgaagcac ggatagtttt gtatcctgca aaatcaacca accttgccaa
     2281 cacttcaccc aagatcgtat tgcgcgcatg cccaatatgg aatgagtgat gcgtgttcgg
     2341 ctgtgcatat tcaaccatca ccgtttccgg ttttgcttcg ccacgcccaa attcagcacc
     2401 gtttttgagc acttcttcca caacacgatg agcatattca gatgtcttaa agtagacgtt
     2461 gagatagccc ttcaccgctt caatgtgact gatacccgcc acgcttccta attgaccttt
     2521 gacctgctcg gcaatttcct gagctttttg cggcactggg gcttttgtcc ctttgccgag
     2581 ttttgcttcg ttagcagcgg tttggaagaa cgaagtagcg aaaccccatt cacccgagaa
     2641 ggggatgggt tgccacttta attctgccaa tgcaatgtaa ttggcggcac aatattcttt
     2701 aattttttct tcgatgagtt gttgttcttt ttgaaacatg attgctcaga ttatatcaac
     2761 tacagataac aaaaacggga gtcttgcgac tcccgttgcg attcgagtgc taattacgcg
     2821 ctctttttgg tcaacgctgc aagggctttc atcaagcggc tcttgcgacg cgcgacggtg
     2881 ttcttgtgca acacaccttt ttcagcagcc ttatccaaag cgctgatggc ttgcataaca
     2941 gcctgcttgg attcgggggt gtcgctggtg agaactttgc gcgcagcgtt atcggcagta
     3001 cgggcggcgc cacggacatt gcggttacgc aggcgacgct tctcgttctg acggtttcgt
     3061 ttgatctgag attgaatgtt agccaaggtc ttcctccaaa aaattcttgc cttactttac
     3121 gacggcgacc tattttacat ggcacaggca tatttgtcca gtaaaatgat aagaaaaccg
     3181 cgggcaattt gcccgcggtt tttgtttatg gggtggctgt tgccggcgcc gcattaatgg
     3241 gggtggaggt gccggttggt aaaacggttc ctggtcccgg tgtgatgggg gtgcttgtgg
     3301 gtgggcgggt ggcggtaggt gtcaccatgt ttacaggaat taccaaaacc tgtccaacaa
     3361 aaagggcatt ggggtctgta atattgtttt ccttcataat atcgtcaata gtgctattga
     3421 aggcattggc aatggcacct aaagagtc
//
LOCUS       DAOQJE010000311         3436 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_17630_length_3436_cov_6.959775, whole genome shotgun sequence.
ACCESSION   DAOQJE010000311 DAOQJE010000000
VERSION     DAOQJE010000311.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3436)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3436)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3436
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17630_length_3436_cov_6.959775"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1477)
                     /locus_tag="PLT08_09130"
     CDS             complement(<1..1477)
                     /locus_tag="PLT08_09130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HNE04668.1"
                     /translation="MKEYTTEFIRNVALVSHGGAGKTMLAEAFLHVTGATTRLGKVED
                     GTTVSDYDDEEHKRKISIYTSVIPVEHKNYKINFLDAPGYTDFVGEMVSALSVADGAI
                     ILVDAVAGIEVGTELAWRYCDQFNLPRFFVINKMDRENADFQKTYDAITEFVEAHGKR
                     VVQVHIPIGEKQNFKGVVDVIGMKSYMGDGKTIAEIPAELKEAADKAHFAMVEDAAEG
                     EDQLMEKYLENGSLSDEEMVRGLEDVVYAGQFVPVFCAAGAHEIGTIALLNDIIDLIP
                     SPAQAVKRMAQGKDGAEELKASDQGPLAAYVWKTTADPFVGKMTYFRVLSGSLQADAH
                     VWNQNKGADERMSGMHFQRGKETIAAKSIHAGDLAVVSKLTVTATGDTFCDKGHPLTV
                     EKPAYPAALYRVAVAPKTQADAAKISTVLTRLSEEDMTLTWQNDPVTHETVLQGMGDQ
                     HIDVAVHRAQNKFQVGIVTHEPKVPYREGITRKAQAQYRHKK"
     gene            1599..2090
                     /locus_tag="PLT08_09135"
     CDS             1599..2090
                     /locus_tag="PLT08_09135"
                     /inference="COORDINATES: protein motif:HMM:NF012314.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HNE04669.1"
                     /translation="MGDIFGLYSFVFIAVGFAFVAGVILLSNKPKWNDYLAFGVIVGG
                     LLVAWTILHPRQTLLMDDAKQVKDMIGAGTPVLLEFQSPYCIRCVSIKPIVDELEEEI
                     DPADIHIIRLNIQEQIGMELAPLYNFVATPTFIYFDSEGNEIWRMVGDFDPQRVRDSL
                     ANE"
     gene            2083..2658
                     /locus_tag="PLT08_09140"
     CDS             2083..2658
                     /locus_tag="PLT08_09140"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF017534.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNE04670.1"
                     /translation="MNNLFRRLKFNYNYIAKKTPWDSGISPPELYDFMAAHSPGRALD
                     IGCGTGTNLITLANAGWQAEGFDFASRAVTIAKRKIQKAGVTVKVFTDDATQMKQVTP
                     PFDLALDLGCFHSITNKADYLTQLTRVLNPSGFWLVYGFIKPDPALTGPGITTADLDL
                     IQRHNLTLVSRKDGFDRRDRISAWFLWQANS"
     gene            2702..>3436
                     /locus_tag="PLT08_09145"
     CDS             2702..>3436
                     /locus_tag="PLT08_09145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE04671.1"
                     /translation="MKILFLFLDGIGLGEDNPDTNPFLRANMPYLESLLGGKKMTKSA
                     APFESDVLSLKAIDPNLGVKGLPQSATGQAVLVTGINIPAELGYHYGPKPNPEVAQYL
                     NGHTIFSKTVNAGKKATLLNAYPPRYFDGIDSGKRLYSSIPLALTNAGISLFTEKDYF
                     TGTALSADFTGEGWREFLGFPDAPLFDPETAGTKLAELASQYDFSFFEYWASDYAGHK
                     QDMPWAIKQLEVFDGVLKGLLTNWDME"
BASE COUNT          872 a          919 c          747 g          898 t
ORIGIN      
        1 gtttcttgtg gcgatattgc gcttgggctt ttctcgtaat cccttcgcga tatggaacct
       61 tcggctcatg cgtaacgatg cccacttgga atttattttg agcgcgatgg actgctacat
      121 cgatgtgctg atcccccatc ccttgcaaga cggtttcatg agtcacaggg tcgttttgcc
      181 aagtgagggt catgtcttct tcgctcaagc gggtgagcac ggtggaaatc tttgccgcat
      241 cggcttgggt cttgggagct actgccacgc gatacaaggc tgcaggataa gcaggttttt
      301 ccactgtgag tggatgcccc ttatcacaaa aagtatcgcc agtcgcagtg acggtcaact
      361 tggaaacgac cgcaagatca cccgcatgga tgctcttggc agcaatcgtt tcttttccgc
      421 gctggaagtg cataccagac atgcgctcat ctgcgccctt gttttgattc cagacatgcg
      481 catctgcttg tagcgagcca gaaagcacgc ggaaataggt catctttccg acaaaggggt
      541 cggcggtggt cttccaaaca taagcagcca atggaccttg gtctgaggct ttcaattctt
      601 cagcgccatc tttgccttgt gccatacgct tcacagcctg agccggtgat gggatcaaat
      661 caatgatgtc attgagcaaa gcaatggtgc caatttcatg cgcacccgct gcacaaaaga
      721 ccggtacaaa ttgtccggcg tagaccacat cttcgagacc acgcaccatt tcctcgtctg
      781 agagagagcc gttctcgaga tatttttcca tcaattggtc ttcaccttcg gcggcatctt
      841 ccaccatagc gaagtgagcc ttatccgcag cttcttttaa ctcagcaggg atttcggcga
      901 tggtcttccc atcccccata tacgatttca tgccgatgac atcgaccaca cctttgaaat
      961 tttgcttctc gccgatggga atgtgaactt gaacaacgcg ttttccatgc gcttccacaa
     1021 attcagtgat ggcatcgtat gttttttgga aatccgcatt ttcacgatcc attttgttga
     1081 tcacaaagaa acgcggcaaa ttgaattgat cgcaatagcg ccaggcaagt tcggtaccta
     1141 cttcaatgcc cgcaacggcg tctactaaaa taattgcgcc atctgccaca ctcaaggcgg
     1201 aaaccatctc gcctacaaaa tcagtgtagc ctggcgcatc aagaaaattt attttgtaat
     1261 tcttgtgttc aaccgggatc acgctcgtat aaatcgatat ttttcgttta tgttcttcgt
     1321 cgtcgtaatc cgatactgta gtcccgtctt ccacctttcc aagacgggtt gtcgcccccg
     1381 taacatgcag gaatgcctct gcaagcatgg tcttccctgc tccgccgtgc gaaacaagcg
     1441 caacgttgcg gataaactcg gtggtatact ctttcataag taagttacct tcctgttctt
     1501 ataagattgt gatgcaggtt gagtgattgt aaaagaactt aaatgaattg tcaaagtgtg
     1561 aagcgtcaca caaaaaatat gacattgtag actagataat gggcgatatt tttgggcttt
     1621 attcttttgt ttttatagcg gtagggtttg cattcgtcgc cggggtcatt ttgctctcga
     1681 acaaacccaa atggaacgat tatctagcat tcggcgtcat cgtaggcggg ttgctcgtag
     1741 catggacgat cctgcatccg cgtcaaaccc tcttaatgga tgatgccaag caagtgaaag
     1801 acatgatcgg cgccggcaca cccgtcctgc tcgaattcca gtccccctac tgtatacgct
     1861 gtgtatccat caaacccatt gtggatgaac ttgaagaaga aatagaccca gccgatattc
     1921 acatcattcg tttaaacatc caagaacaga tcggcatgga actggcgcca ttatataatt
     1981 ttgtcgccac gcctacattt atatactttg actcagaagg caacgaaatt tggcgcatgg
     2041 tcggcgattt tgacccgcag cgtgtgcgcg actctcttgc aaatgaataa cctcttccgt
     2101 cgattaaaat tcaactacaa ctacattgct aaaaaaactc cgtgggatag cggcatctct
     2161 ccaccagagt tatacgactt catggcagcc cactcccccg gtcgcgcctt ggatattggt
     2221 tgcggcacag gcaccaacct catcaccctg gcaaatgccg gttggcaagc cgaaggcttc
     2281 gacttcgcct cacgtgccgt cacaatagcc aaaagaaaaa tccaaaaagc cggtgtcaca
     2341 gtaaaggtct ttaccgacga cgccacacaa atgaaacaag tcacaccgcc tttcgatcta
     2401 gccctcgacc tcggctgttt tcacagtatt acaaacaaag ccgattatct gactcagtta
     2461 actcgcgtgc taaacccaag cggattttgg ctcgtctacg gattcatcaa accagacccg
     2521 gctcttactg gacccggaat taccaccgct gacctagacc taatccaacg ccataacctc
     2581 acactcgtct cccgaaaaga cggcttcgac cgacgcgacc gtatctctgc ctggtttttg
     2641 tggcaggcaa attcgtaagg ctttttacgc attacacatc atgtaatacg taaacctacc
     2701 tatgaaaatc ctctttctct tccttgatgg tatcggctta ggcgaagaca acccagacac
     2761 caatcccttt ttgcgcgcta atatgcctta tctcgaatcg ttacttggcg gcaagaaaat
     2821 gaccaagtcc gctgcgcctt tcgagagcga cgttctctct ttgaaagcca tcgatccaaa
     2881 tctgggtgta aaagggctgc cccaatcagc cacagggcaa gcagttcttg tcacaggcat
     2941 caacatccct gctgaacttg gttaccatta cggaccaaaa cccaaccctg aagtggcaca
     3001 atatctcaat ggtcacacca tcttttcaaa gactgtaaat gccggcaaaa aagcgacgct
     3061 tctcaatgcc tatcctccac gctactttga tggaattgat tcaggcaaac gcttgtattc
     3121 atctattcca ttggcactaa ccaatgcggg catttccctc ttcactgaga aagattactt
     3181 cacaggcacc gccctctctg cagatttcac cggcgaaggc tggcgagaat tcctcggttt
     3241 ccccgatgcg ccactcttcg acccagaaac cgctggcaca aaacttgctg aacttgccag
     3301 ccaatatgat ttttctttct ttgaatattg ggcaagcgac tacgcggggc acaaacaaga
     3361 tatgccatgg gcaatcaaac aacttgaagt attcgatggc gtactcaaag gtctgctcac
     3421 gaattgggat atggaa
//
LOCUS       DAOQJE010000312         3433 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_17654_length_3433_cov_6.814979, whole genome shotgun sequence.
ACCESSION   DAOQJE010000312 DAOQJE010000000
VERSION     DAOQJE010000312.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3433)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3433)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3433
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17654_length_3433_cov_6.814979"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..584
                     /locus_tag="PLT08_09150"
     CDS             <1..584
                     /locus_tag="PLT08_09150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE04672.1"
                     /translation="ANPSADSHLPYYWGKAANEKAVTESGMSYAILRPTVLVGGGEDI
                     LINNIAFLLKRFPIFFVPGDGQYGIQPVHVEDLAKLAVEGVYSKENYVIDAVGPDSYT
                     FKGLIELVRDTIGAKSLLISMPPRLALLAAQFLSLFVNDVILTPEEVDGLMGNLLVSK
                     EPAHGKTLFKNWLKENREIVGATYASELKKHYV"
     gene            complement(601..1182)
                     /locus_tag="PLT08_09155"
     CDS             complement(601..1182)
                     /locus_tag="PLT08_09155"
                     /inference="COORDINATES: protein motif:HMM:NF022337.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2726 domain-containing protein"
                     /protein_id="HNE04673.1"
                     /translation="MTEDKSSRLPYRLREPFLSNTEAALFRLLTDLMGDRYMICPKVA
                     LTDLFMIVRPNENVHFFNKIFRKHVDFLLCDPKTFTPTFGVEIVKSIAKDGMREADKF
                     LAELFTDAGIPLVHIPTNDKYEPADLIALFQLAVLKVGKNIPVEVAMDSVPSCPVCGK
                     MMVLRMHRNGNSAGKKYYGCIDNPTCAGKVAID"
     gene            complement(1228..1992)
                     /locus_tag="PLT08_09160"
     CDS             complement(1228..1992)
                     /locus_tag="PLT08_09160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0046855 - inositol phosphate
                     dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HNE04674.1"
                     /translation="MNLQPYLEFATSLAYHAGKITLRYFNTGIVPDRKENNDPVTIAD
                     REAEAFVRSQVEKHYPGHAIVGEEYGEQGTGHSFRWIVDPIDGTISFVQGVPLYGVLI
                     ALEIDGVVQVGAAYFPPLDEMLTAGSGLGCWWNGRRARVSGVSKLSEACVVTSDFQHL
                     EKKQAGITKRFEESGALLRTWGDAYGYLLVATGRAEVALDPRMDVYDCGPYPVIMKEA
                     GGYFGSWIGEEGHDHGEGLACNAALKPDVMKLMRGK"
     gene            complement(1989..3230)
                     /locus_tag="PLT08_09165"
     CDS             complement(1989..3230)
                     /locus_tag="PLT08_09165"
                     /inference="COORDINATES: protein motif:HMM:NF013871.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE04675.1"
                     /translation="MQKDDVLRLLRDPNLIRPEKLTLLSNIADLAASLNMPCYVVGGF
                     VRDLLLQKPVNDLDVIVEGDAIKLGKKLVEAYGGKLTTHFKFNTAIWHLPDSTDFVDL
                     ITARKETYEKPGALPTVTPSTMEDDLRRRDFTINAMALRLDGEGFGEILDPLKGQTDL
                     ERGVIRVLHPNSFIDDPTRIFRAIRYEARYGFSMDEETSGLVNTEALKVLHSLSGERI
                     RHELDLILDEDESSKMMLRIVNLGVLSAIHHKMPEFNQDYADFLEMDVRLDVQADRRT
                     MGYILWFMDLSEEDVILLSNRLDFTNELTLAVWAGAQLRRSLIHLVGARPSEWTYALE
                     KLPLLSIYVVYLVSRENDLLSYISLWRHVKPQTTGNDLKAKGLLPGPRFSEILLRLRS
                     AWLDGEVNNESQEKELLAKLL"
BASE COUNT          902 a          896 c          770 g          865 t
ORIGIN      
        1 tcgcaaaccc atctgcagat tcgcatctgc cttattattg gggaaaagct gccaacgaaa
       61 aagctgtgac cgagtctgga atgtcctatg ccatcctgcg cccaacggtg ctagtcggcg
      121 gaggcgaaga tatcctcatc aacaatattg cgtttctctt gaagcgcttc cccattttct
      181 ttgtccccgg cgatgggcag tatggtattc aaccagtgca tgtcgaagac ctcgcgaagt
      241 tggctgttga aggggtttat agcaaagaaa attatgtgat tgacgctgtg ggtccagatt
      301 cgtacacatt caaaggactg atcgaattag tcagagatac gattggcgca aagagtttat
      361 tgatctccat gccgccacgt ctggcactac tcgccgctca attcttgagt cttttcgtca
      421 atgacgtgat tctcacccct gaagaagtgg atggattaat gggaaatctg ctggtttcca
      481 aagagccagc gcatggcaaa accttgttca aaaattggct aaaggaaaat cgtgagatag
      541 ttggtgcaac ctatgcatct gaattaaaaa agcattacgt ctaatacccc caaaagcaac
      601 ctaatcgatg gctactttgc ctgcacaggt tgggttatcg atgcatccgt aatatttttt
      661 tccggcgctg ttgccattac gatgcatacg caaaaccatc atcttgccgc aaaccgggca
      721 gcttggcacc gaatccatcg caacctctac tggaatattc ttccccactt tcaacaccgc
      781 cagttggaac aatgcaatca aatctgctgg ttcatattta tcgttcgtag gaatgtgaac
      841 cagcggaatt ccagcatcgg taaaaagttc tgctaaaaat ttatcggctt cgcgcatgcc
      901 atcttttgca atcgacttga cgatctccac cccaaaggtg ggagtgaagg tcttcgggtc
      961 gcacaataga aaatcaacat gcttgcggaa aatcttatta aaaaagtgaa cgttttcgtt
     1021 cgggcgtacg atcatgaaca agtctgtcag agcaaccttg gggcaaatca tataacgatc
     1081 acccatcaga tccgtcaaca aacgaaaaag agccgcttcc gtattgctta ggaagggctc
     1141 tcgaagacgg taaggaagtc ggctgctttt atcctcggtc ataattcgat tatacgttag
     1201 gtttgaatct acgtacagaa atttgaccta ctttccgcgc atcaatttca taacatctgg
     1261 cttgagtgcg gcgttacaag ccaacccttc gccgtggtca tgaccttctt cgccaatcca
     1321 cgaaccgaaa taaccacctg cttctttcat aatcaccggg tacggaccgc aatcatacac
     1381 atccatgcgt gggtctagtg ccacttctgc acgcccagta gcgaccaata aatatccata
     1441 agcatcaccc cacgtccgca gcagagcgcc gctttcttca aaacgttttg taatgcccgc
     1501 ctgcttcttt tcaaggtgct gaaaatctga agtgaccaca caggcttctg aaagtttact
     1561 cacgcctgaa acacgtgcgc ggcgtccatt ccaccaacag cccagcccgc tgcccgctgt
     1621 caacatctcg tccagcggcg ggaagtaagc agctcctacc tgcaccacgc catcaatctc
     1681 cagagcgatt aacaccccat ataaaggaac accctgcaca aacgagatcg tcccatcaat
     1741 ggggtctacg atccagcgga atgagtgacc cgttccctgc tcgccatatt cctcccccac
     1801 aatggcatga cccggataat gcttttccac ctgcgagcgc acgaaagctt cggcttcgcg
     1861 gtcggcaatg gtcacagggt cattgttttc tttacggtct ggcacaatgc ctgtgttgaa
     1921 gtaccttaaa gtaatcttcc ccgcgtgata cgcaagggaa gtggcgaatt cgagataggg
     1981 ctgaaggttc atagaagttt ggcaagcaat tccttttctt gagattcatt gttcacttcc
     2041 ccatccaacc aggcagagcg caaacgtaat aagatctcgc taaaacgcgg acccggcaac
     2101 aagccttttg ccttgagatc attccccgta gtctgtggtt tgacatgccg ccagagtgag
     2161 atataactca acaaatcatt ctcacgtgag accagataca ccacataaat agaaagtagc
     2221 ggcagctttt ctaaagcata ggtccattca ctggggcgcg cgccaacaag atgaatcaag
     2281 ctacgtctca attgcgcccc cgcccaaacc gccagcgtca gttcattcgt aaaatccaag
     2341 cggttcgaaa gcaagatcac atcttcttcc gataaatcca taaaccataa gatgtaaccc
     2401 atcgtgcgcc ggtcggcttg tacatctaaa cgaacatcca tctcaagaaa atcagcataa
     2461 tcttgattga actctggcat cttgtggtga atagcactca acacgcccaa gttgactatg
     2521 cgcagcatca tttttgagga ttcatcttca tccagaatca aatccaattc atggcggatg
     2581 cgttctccgc taagcgaatg caaaaccttc aacgcctccg tattgactaa gccagaggtt
     2641 tcctcatcca tcgaaaatcc atagcgtgct tcgtagcgaa tggcgcgaaa gatgcgggtt
     2701 gggtcatcaa taaacgaatt cggatgtaga acgcggatca ctccccgctc gaggtctgtt
     2761 tgccctttca gcgggtcgag gatttcgccg aaaccttcac cgtccaaacg cagtgccata
     2821 gcgttgatag taaagtcgcg gcggcgtaaa tcatcttcca tcgttgacgg tgtgaccgtt
     2881 ggcaatgcgc cgggcttttc gtaggtttct ttgcgagccg tgatgaggtc gacaaaatcg
     2941 gttgaatctg gcaaatgcca gatcgcggtg ttgaatttga agtgtgttgt gagcttccca
     3001 ccatacgctt cgaccagttt tttcccaagt ttgatggcat cgccttcgac gataacatcc
     3061 aaatcgttca ctggtttttg aagcagtaag tctcggacga agccgcccac cacatagcac
     3121 ggcatgttca aggatgcggc taggtcggcg atgttcgaga gaagggtcag cttctcgggg
     3181 cgaatcaggt tcgggtcccg taagaggcgc agcacgtcat ccttttgcat aaattcaatt
     3241 ttacacgtat atatgcccaa gttcaaaaat gcgcccttat gtaatttttg aacgttgggt
     3301 ataatcagaa aatgtttatg aacccaaacc cgtcccctcg aagttgtaca tcctccaggt
     3361 ctggctaggt tcactttcgt gcgcccaaac ggctcccaga cttggtgagc cgttttttat
     3421 tggatggtga aga
//
LOCUS       DAOQJE010000313         3433 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_17655_length_3433_cov_5.762581, whole genome shotgun sequence.
ACCESSION   DAOQJE010000313 DAOQJE010000000
VERSION     DAOQJE010000313.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3433)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3433)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3433
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17655_length_3433_cov_5.762581"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..393
                     /locus_tag="PLT08_09170"
     CDS             <1..393
                     /locus_tag="PLT08_09170"
                     /inference="COORDINATES: protein motif:HMM:NF013614.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SufD family Fe-S cluster assembly protein"
                     /protein_id="HNE04676.1"
                     /translation="MAPHCTSDLLFKGALKGKSRSVWQGMIYVAPGAQKTDGYQANRN
                     LVLDDQARADSIPGLEILADDVRCTHGATVGKLEQEPLFYLKSRGIPQKEAEQLVVEG
                     FFDPIMQRIPFEGVRERFQQAIQIKLTA"
     gene            528..1133
                     /locus_tag="PLT08_09175"
     CDS             528..1133
                     /locus_tag="PLT08_09175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE04677.1"
                     /translation="MAVKSKTKSNSKTKVVVKAKTATKKGTVKVSAARKVVSKSKTKV
                     VAKSVKPSKSVVSSIKKKPASAEKPVEVTRTINLSSQIRQPAKPVLRRSSVGLGATNS
                     NPFTFMKAPRPEYAFEVGDTVEVFCDHEKGRERVRGWIKGIVVQVDNKMVAVQFRSNV
                     FLTDGWMVPDRILWYPLNSEHIRPGTTGKKPALKKDIIPDY"
     gene            1211..2437
                     /locus_tag="PLT08_09180"
     CDS             1211..2437
                     /locus_tag="PLT08_09180"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0006534 - cysteine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HNE04678.1"
                     /translation="MTLNINEIRKDFPILERETANGKRVIYLDSTATSQKPLQVIEAM
                     NDYYRRSNANIHRGVHTLAEEATSLYEGARERIAKFIHAATSREIIYTRNTTESINLV
                     AYSWARANLKAGDLVILTEMEHHSNLVPWHMLQAERGIELEFIPVTEDGLLDLEAYKT
                     LLNRTPKLVSFTHMSNVLGTINPAAEIIKLAHAAGAVTLVDGAQSVPHLAVDVQALDA
                     DFYAFSAHKMCGPTGMGVLYGKSALLEAMPPFLGGGDMIKEVKLRSFRPNTLPHKFEA
                     GTPAIAEAIGLGAAVDYLTQIGISDIAAHEHEITEYALERLEEIPGVKLFGPSADKKG
                     GVAAFTLEGVHPHDVAQILDQDGIAVRAGHHCAQPLHEKFGIPATSRASFYLYSTKDE
                     VDMLVKGLYKVKEMFG"
     gene            2486..2881
                     /locus_tag="PLT08_09185"
     CDS             2486..2881
                     /locus_tag="PLT08_09185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SUF system NifU family Fe-S cluster assembly
                     protein"
                     /protein_id="HNE04679.1"
                     /translation="MDDLYREVIIEHYKNPSYRGKLDPHDISFADNNPLCGDHIQIDL
                     RVDANGVVSEARFDGHGCAISQASADLLMETIIGKPLEEVKKLNRQDILDMLGIDLGP
                     VRLKCALLSLKVLKAGVYGLGEAGDDLVE"
     gene            2887..3327
                     /locus_tag="PLT08_09190"
     CDS             2887..3327
                     /locus_tag="PLT08_09190"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNE04680.1"
                     /translation="MASYQIREMVIDDYDAIIVMFHETPGITVRDADSRESTAAYLMR
                     NPGLSFVATINGKIIGCVMSGHDGRRGYLQHLIVTPEFRRQGIGKALYLACLAALQKI
                     GIYKTHLFVQKSNSSGEEFWREQGWVFRNEVLMFSFNASPNENI"
BASE COUNT          886 a          758 c          891 g          898 t
ORIGIN      
        1 atggctccgc actgcaccag cgaccttttg ttcaaaggtg cgcttaaagg aaagagccgc
       61 tcggtgtggc agggtatgat ctatgttgcc cccggtgccc agaagacgga tggctaccaa
      121 gccaaccgta acctcgtcct cgatgaccag gcacgcgcgg attctattcc gggtctcgaa
      181 attctcgccg acgatgtgcg ctgtacacat ggcgcaaccg ttggtaaact tgaacaggaa
      241 cccctgtttt acctgaaatc tcgtggtatt ccgcaaaaag aggcggagca gttggtggtg
      301 gaaggtttct ttgaccccat catgcaacgc atcccgtttg aaggtgtgcg tgagcgtttc
      361 cagcaggcga tccagatcaa gttgacagcg taatttgttt tctctcgctg gggtgtacac
      421 cccagcgaga gaattatcac cgtaagaaaa tgcctgaagc gttgcatcag gcatccttta
      481 agaatgaggc ggagggcgtc tcaattttcg ttcgtaggaa gtgagctatg gcagtgaagt
      541 ctaaaacaaa atccaattcg aagacaaaag tggtggtaaa agcgaaaacg gcaaccaaaa
      601 aggggacggt taaggttagt gcggctcgta aagtagtttc aaaatcgaag acaaaagtgg
      661 ttgcaaaaag tgtcaagcct agtaagtcgg ttgtttcttc tataaagaag aagccagctt
      721 ctgctgaaaa accggttgaa gtgacgcgca ccattaactt gtcgagccag atccgtcagc
      781 ctgcgaaacc tgtcttgcgc cgctcttctg ttgggttggg tgcgacgaac tccaatccgt
      841 tcacgtttat gaaggcgcca cgccctgagt atgcttttga agtgggtgat acggtagaag
      901 tgttctgtga tcacgaaaag ggacgcgaac gtgttcgtgg ttggatcaaa ggcattgtgg
      961 ttcaagtgga taacaaaatg gtggcagtgc agttccgctc gaacgttttc cttacagacg
     1021 gttggatggt gccagatcgc attctttggt acccgctgaa ttctgagcac attcgccctg
     1081 gaaccaccgg caagaaaccc gccttgaaga aagatattat tccggattat taagccaata
     1141 cgcaggtaga caggtacaca agcaaaaaat agacatgtgt acttgtgtac ctgtctattt
     1201 tttatttccc atgacgctaa acataaacga aattcgtaaa gatttcccta ttctagagcg
     1261 cgaaactgcc aatggcaagc gcgtgatcta tcttgactcc accgcaactt ctcagaagcc
     1321 tttgcaagtg atcgaagcga tgaacgatta ttatcgccgt tcaaatgcaa atattcaccg
     1381 tggtgtgcat actttggctg aagaagccac ttctttgtat gaaggtgcgc gtgaacgcat
     1441 tgccaaattt atccatgcgg caacttcacg tgagattatt tacacgcgta atacaaccga
     1501 gtcgattaat cttgtcgctt attcttgggc gcgtgcaaat ttaaaggcgg gcgaccttgt
     1561 tattctgacc gagatggaac accatagcaa tctggttccg tggcatatgc tgcaagcaga
     1621 gcgtgggatt gagctggaat tcatccctgt gacggaagac ggtttgctgg atctggaagc
     1681 ttataaaacg cttctcaacc ggaccccgaa actggtctcg tttacacaca tgtccaacgt
     1741 gttgggcacg atcaacccgg cggcagagat catcaaatta gctcatgcgg cgggtgctgt
     1801 cactctagtg gatggcgctc aatcagtccc tcacttagcc gtggatgttc aagccttgga
     1861 tgctgatttt tatgcgttct ctgcgcataa gatgtgtggt cccaccggga tgggtgtgtt
     1921 gtatggcaag tcagctttgt tagaagccat gcctccgttc cttggtggtg gtgacatgat
     1981 caaggaagta aaactgcgtt ccttccgccc gaacactttg ccacataaat ttgaagcggg
     2041 tacacctgcc atcgcagaag ccatcggttt aggtgctgct gttgattatt tgacccagat
     2101 cggcataagc gatattgctg cccatgagca tgagatcact gaatatgcgc ttgaacgctt
     2161 ggaagaaatc cctggtgtaa agctgtttgg tccctctgcg gataaaaaag gtggcgtagc
     2221 tgcttttaca cttgaaggcg tgcaccccca tgatgtggcg caaattttgg accaggatgg
     2281 tatcgccgtc cgcgcagggc accactgtgc tcagcctttg catgaaaagt ttggcatccc
     2341 tgctactagc cgagcttctt tctatttgta ctctacaaaa gatgaagtgg atatgctggt
     2401 aaagggttta tataaagtaa aagaaatgtt cgggtaatta tggagcgagg tctcctcgcc
     2461 ctgtttccct gaaacggaat atataatgga cgatctctat cgtgaagtaa tcattgagca
     2521 ctataaaaat ccatcctatc gtggaaagtt agacccgcac gatatttcct ttgcagataa
     2581 caacccatta tgcggcgacc atatccaaat cgatttgcga gtggatgcga atggtgttgt
     2641 cagcgaggca cggtttgatg ggcatggttg tgcgatctcg caagcctcgg ctgacctgtt
     2701 aatggagact attatcggga agccgctgga agaagtaaaa aagctcaacc ggcaggatat
     2761 tcttgatatg ctcggtattg accttggacc ggtgcgtttg aagtgtgcat tgctttcgct
     2821 taaagtgttg aaggctggag tatatggctt aggggaagcc ggagacgatt tggtggagta
     2881 agaagcgtgg cttcatatca gatccgcgag atggtcattg atgattacga tgccatcatt
     2941 gtgatgtttc atgagacgcc gggcatcacg gtacgagacg cagactcgcg agaatcaaca
     3001 gccgcttatt tgatgagaaa ccccgggctg agcttcgtag ccaccataaa tggaaagatt
     3061 atcggctgtg taatgtctgg gcatgacggg cggcgcggct atttgcaaca cctgatcgta
     3121 acgcctgaat ttcgaagaca aggcattggc aaggctttgt atttagcttg ccttgctgcc
     3181 cttcagaaga tcggtatcta taagacccat ctttttgtac aaaagtcgaa ttcttctgga
     3241 gaagagtttt ggagagagca gggctgggtc tttcgaaatg aagtgctgat gttttctttc
     3301 aatgcatctc caaatgaaaa tatatgaaat ctgagaacaa ctatgtttaa ttacacctcc
     3361 cttgatgaat caaaagttga atttgtagaa attgcccctg cttccgaact cccgaatggg
     3421 gaacgcctct ttg
//
LOCUS       DAOQJE010000314         3430 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_17677_length_3430_cov_5.735704, whole genome shotgun sequence.
ACCESSION   DAOQJE010000314 DAOQJE010000000
VERSION     DAOQJE010000314.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3430)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3430)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3430
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17677_length_3430_cov_5.735704"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..651
                     /locus_tag="PLT08_09195"
     CDS             <1..651
                     /locus_tag="PLT08_09195"
                     /inference="COORDINATES: protein motif:HMM:NF012695.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HNE04681.1"
                     /translation="MPSTGYGYIQQGMPIGGGYTYPVYTVQSFKEKPDEATAQQLLRM
                     GGHSWNSGMFIWRADAILNEIDKQMPNLGKALREIGDTWNSSHKIETLKKIWPDLKNE
                     TVDYGIMEKAEKVAVLPAGGLGWSDVGSWSSLFEVLLPDMSGNIATNDALHLALETNN
                     TMVYGRGSDRLVVTIGVDDTVVVDTGDVLLICKTDQSQKVKDVVEHLKKHRQEKYL"
     gene            820..3111
                     /gene="topA"
                     /locus_tag="PLT08_09200"
     CDS             820..3111
                     /gene="topA"
                     /locus_tag="PLT08_09200"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HNE04682.1"
                     /translation="MKCKTKREMQDPVAGFNAKSSAVTTGTCPVCGTKLFRMGKTDAH
                     AGLIPPPKPEKVEVREGKLVIVESPAKAKTVGRFLGKGYTVRASVGHVRDLLKSQLSV
                     DVENNFEPKYRVPNEKKDVVKEIKQLAKKAEEIFLATDPDREGESISWHLMEAAGIEP
                     ERTKRVVFHEITAPAVADAFSHPRNIDMNLVNAQQARRVLDRLVGYSISPILWEKVRG
                     RLSAGRVQSVALRLIVDREREIDAFKPVEYWTIHAEFKPEKLKSNFIAKLVRVDDKEP
                     ELPSKETVEPLLYDLETASFSISKVKRGERRRKPAAPFTTSTLQQEASRKLGYTARRT
                     MALAQGLYEGQDVGEGGTTGLITYMRTDSTNVSAIAQSETREFVTGKYGGDFLPAEPP
                     TYKTKSANAQEAHEAIRPTSVMRDPEKVKEFLEPAMYKLYRLIWQRFVASQMEAAVYD
                     TLQVEVTGKTNEHEYLLRASGSAIKFPGFMVVYEEAKNEDAKVEEDEEDVKIPAGVAE
                     GQKQELVRLIPEQHFTQPPPRFSEASLVQALEENGIGRPSTYAPTISTIQQRGYALRE
                     EKRLVPTDMGIQVNDLMAQYFPEVVDLNFTAHMEEDLDKIADGQMEWVDAIREFYGPF
                     AEDVKKAQAEMPVAKSEPEPIGRKCPDDGGELVIRYGRFGKFISCANFPTCRYTEPWL
                     EKIGVSCPKDGGDMVERRTRKGRTFYGCANYPNCDFTTWKKPLAKPCPKCNGLLVIAN
                     KREAQCTNCSESFLLEEIVPENA"
BASE COUNT          909 a          760 c          903 g          858 t
ORIGIN      
        1 atgccttcta ctgggtacgg atatattcag caaggaatgc ctattggcgg cggatacaca
       61 taccctgttt atacggtgca aagtttcaag gaaaaaccag acgaggctac cgcgcaacaa
      121 ttgctccgta tgggggggca ctcctggaat agcggcatgt tcatatggcg tgccgatgcg
      181 atcttgaatg agattgataa acagatgccc aacttgggca aagccctgcg tgaaattggc
      241 gatacgtgga attcttctca taagattgag accttgaaaa aaatctggcc cgacctgaag
      301 aatgaaactg tggactatgg cattatggaa aaagccgaaa aagttgccgt cttgcctgct
      361 ggcggtttgg ggtggagcga tgttggttca tggtcttcgc tattcgaagt ccttctgcct
      421 gatatgagtg gcaatattgc caccaatgat gctttgcatt tagcgcttga aaccaacaac
      481 acaatggtct acggacgtgg atctgatcgc ctcgttgtga ccattggagt ggacgataca
      541 gtggttgtag atactggcga tgtgctgttg atatgcaaga cagaccagtc acaaaaggtt
      601 aaagatgtag tggaacatct caaaaaacat cgccaagaaa agtacctcta aaatcgcccg
      661 aaaaattggc gtttgcaaaa aagcggaaac ttgttgtaga atgtccatcc cgtaaggttt
      721 aaccttgcgg gatgtttttt atacgaaccg aacaactctc gaggcaaaaa tctgctttgt
      781 gagagatatg ttggaggaat acaaatggaa gcttattgta tgaagtgcaa gaccaaacgg
      841 gaaatgcaag atcctgtggc aggtttcaat gccaagtcat ctgccgtgac aacagggact
      901 tgcccggttt gtggcacaaa attatttcgc atgggcaaga cagatgctca tgctggtttg
      961 attccgccgc ccaagcctga aaaggtggaa gtgcgagaag gcaaacttgt aattgttgag
     1021 tcgcctgcca aggcaaaaac ggtggggcgt tttcttggaa agggctatac cgtccgtgct
     1081 tcggtggggc atgtgcgtga ccttttgaag tctcagcttt ctgtggatgt ggaaaataat
     1141 ttcgagccga aatatcgcgt tcccaatgaa aagaaagatg ttgtcaaaga aattaaacag
     1201 cttgcgaaaa aagcggaaga aatctttctc gcaaccgatc ccgaccgcga aggtgagtcg
     1261 atttcatggc atctgatgga agcagctggc attgagcccg agcgtaccaa acgcgtggtc
     1321 ttccatgaaa ttacagcccc tgccgttgca gatgctttct cgcatccgcg taatattgac
     1381 atgaacttgg tcaatgccca acaagcgcgc cgcgtgttgg atcgccttgt gggctatagc
     1441 atcagcccca ttttgtggga aaaggtccgc gggcgcctct ctgcaggtcg tgtgcagtct
     1501 gtcgctttgc gcctcatcgt ggaccgtgag cgcgagatcg atgctttcaa accggttgag
     1561 tattggacga tccatgctga attcaaacct gaaaaactaa aatcgaactt tatcgccaaa
     1621 ctcgtgcgtg tggatgataa agagccggaa ttacccagca aggagactgt agagccgctt
     1681 ctttacgacc tcgaaactgc ttctttttcc atttcaaagg tcaaacgcgg agagcgtcgc
     1741 cgcaaaccgg ctgcgccatt tacaacgtct acgttacaac aggaagcgtc ccgtaaatta
     1801 ggctacacag cccgtcgtac catggcgttg gcgcaggggc tttatgaagg tcaggatgta
     1861 ggcgagggtg gtacgacagg tcttatcact tatatgcgta ccgactcgac taatgtctct
     1921 gctattgctc aaagcgaaac aagagagttt gtcaccggta aatatggcgg cgactttctt
     1981 ccggcagaac cgcctacata caaaaccaag tctgcgaatg cacaggaagc tcacgaagcc
     2041 attcgcccta catctgtgat gcgtgacccg gaaaaggtca aggaattcct tgaacccgcc
     2101 atgtacaagc tatatcgctt gatctggcaa cgctttgttg cctctcagat ggaagccgct
     2161 gtatacgaca ctttgcaggt tgaagtgacc ggcaagacaa acgaacacga atacctcttg
     2221 cgtgcttctg ggtctgctat taagttccct ggctttatgg ttgtttacga agaagccaaa
     2281 aatgaagatg ccaaggttga agaagacgaa gaagatgtaa agattcccgc cggtgtggcg
     2341 gaaggacaaa agcaggaatt ggtccgctta attcccgaac agcactttac tcaacctcct
     2401 ccacgctttt cagaagcatc gcttgtacaa gccctggaag agaatgggat tggtcgtccg
     2461 tctacctatg cgccgactat ttcaaccatt cagcagcgcg gatatgcctt gcgcgaagaa
     2521 aaacgactgg tgccaaccga tatggggatt caggtcaatg acttgatggc tcaatacttc
     2581 cctgaagtag tcgatttgaa ctttacggct cacatggaag aagacctcga taaaattgcg
     2641 gatggacaaa tggaatgggt ggatgccatt cgtgagttct acggtccgtt tgctgaggat
     2701 gtgaaaaagg cacaggcaga aatgcccgtt gctaaatctg agccggagcc gattggacga
     2761 aaatgcccgg atgatggcgg tgagctagtt atccgttatg ggcgcttcgg caagttcatt
     2821 tcctgcgcaa acttcccaac ctgtcgctat acagaaccgt ggttggagaa gatcggtgtt
     2881 tcctgcccga aagatggcgg cgatatggtg gaacgccgca ctcgcaaggg acgcaccttc
     2941 tatggttgtg cgaattaccc gaattgtgat ttcacaacat ggaagaagcc cttagccaaa
     3001 ccttgcccga aatgtaatgg gttgttggtg atagcaaaca aacgcgaggc gcagtgcacg
     3061 aattgttctg aaagtttctt gttggaagaa atcgtgccag agaacgcata aaactttcag
     3121 cgttcgcatc aggcttggtc gtagtggcaa gtatggctga gtctgatgcg tttttttatc
     3181 catgtgggca atcattctgt catacagtgc ccctaattta cgttgtataa tcgagcgaaa
     3241 gcatctgtgc gaaggagcca attatggatt ttgttgttgc atttttaaag aagttgcctg
     3301 ttgtgccaat tgtccttttt gttgtagggt tggcacttgg gctgttgatt ggttggggaa
     3361 tcgcgcccgt agaatttaag gatgcaaccc catattattt gcgccctgat ttgcgcgaag
     3421 attacatgcg
//
LOCUS       DAOQJE010000315         3424 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_17732_length_3424_cov_6.125557, whole genome shotgun sequence.
ACCESSION   DAOQJE010000315 DAOQJE010000000
VERSION     DAOQJE010000315.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3424)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3424)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3424
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17732_length_3424_cov_6.125557"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..376)
                     /locus_tag="PLT08_09205"
     CDS             complement(<1..376)
                     /locus_tag="PLT08_09205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE04683.1"
                     /translation="MIKNQWRSQFPLLLITISILVAGRFFTTDAQAALTINSKTLTPN
                     KAIASWASGIPAGEKRPVIVFLPGWGGVGGVDAAISAQNTNLVNQGYVTLAIGFDSSG
                     TWNSDIEQKTLDGLNKLCADTTI"
     gene            complement(537..1340)
                     /locus_tag="PLT08_09210"
     CDS             complement(537..1340)
                     /locus_tag="PLT08_09210"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HNE04684.1"
                     /translation="MNITGHILIIDDEASIRKTLARILQQAGFEVTTAEGAEQGLDYL
                     RTTQFDLILTDLRMPGMHGMEALKVIHTEHPTLPVILFTAQPDVNSAVEALRHGATDY
                     LLKPIKPEAIIERARNILEKQQQEKRRREIMLQIASLQNELKELDGGEPGKTQPMAPV
                     SFEKERFLKRGKLVLDLHTRRVLLNDEPLNTPPTSFDYLLVLARHAPDVVDYQTLVAE
                     AQGYQTDLREAQELTKWHIHQLRQIIEADARNPVHLINVRGTGYRLVAD"
     gene            complement(1337..>3424)
                     /locus_tag="PLT08_09215"
     CDS             complement(1337..>3424)
                     /locus_tag="PLT08_09215"
                     /inference="COORDINATES: protein motif:HMM:NF024583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HNE04685.1"
                     /translation="CRFDRGGTLTYVNQAYADFFGGTPSELINKSFFDMLDEEQKDLV
                     RSNFYSLTPDKPFIVYEIHEKNCKGEKRWVQWTDRLITNENDIKAEYQSIGMDITDRK
                     LAEIEREHLLEAEHDQRLRAETSAEATLALVSHVELDKVLIEILNQVQRLLPGCGANI
                     ALLENNILRTAAWRGYENRGEEVFKNLVKDTGMYPLDQTMIKNPKTSIIADTALDENW
                     ITIPGLEWIRSHLCIPLLWNKQLLGLLYIDENTPNKFSEETIHRLTPLVNATTVALES
                     ALLIETTRQALKETSALYHINKGMVALDPDELLNDAVNLLKNNFDYYHVQVYVPNPAT
                     GNFYTKAASGAVGKILVEKKHEIRAGSGIVGYAAETGTPFFTNNVDGVMFYLANPLLP
                     DTKSEMAIPVLNGDKLYGILDIQQTSARQFTQRDQQLVLTVADQLAVALHKTELYENL
                     QTALQQEKAVRNQLVQNERLAVMGRLLASVSHELNNPLQAIQNALFLLKEEKGLSQQG
                     LNDLEIVLAESERMASLIERLRETYRPPQVEDLQPAHVNNIIEDVHALLATHLRKNSV
                     AFEFHPDPDIPAIMALPGQIRQVTLNLFMNAVEAMTDGGKLTVSTMGLNETQEIMIAV
                     SDTGPGIAPSILPNIFDPFVTNKKRGTGIGLTISHDIVIKHRGRITAENNPETGATFK
                     VWLPIEPASAELE"
BASE COUNT          799 a          764 c          831 g         1030 t
ORIGIN      
        1 gaatagttgt atctgcacaa agtttattta acccgtccag agtcttttgc tcaatatcac
       61 tattccaagt tcctgatgag tcaaacccaa tggcaagcgt cacatacccc tgattaacca
      121 aattcgtgtt ctgcgccgag attgcagcgt cgactccgcc cactccgccc cagccaggca
      181 ggaacactat cactgggcgt ttttcgccag ccggaattcc agatgcccaa ctggcaattg
      241 ccttattggg tgtcagggtt ttggagttaa tcgttaatgc tgcctgtgca tctgttgtaa
      301 aaaaacgtcc cgctacaagt atcgatatag ttataagtag caacgggaat tgagaacgcc
      361 attgattttt tatcatctct ctgcaccttt atttttgttg gattaagtat agcgattttg
      421 acaggttttt ctatcccacc caaaactcct attgcaggat aacagacccc gcctccggcg
      481 ttgaccggag gcggggttct atccattgag tgagggctac ccttgagttt tatcccctaa
      541 tctgcaacca gtcggtaccc cgtcccgcga acattgatta aatgtaccgg gttgcgcgca
      601 tcggcttcaa tgatctggcg caattgatga atgtgccatt tggtgagttc ttgcgcttcg
      661 cgtaggtcgg tttgatagcc ctgtgcctca gcaacaaggg tctggtaatc gaccacatct
      721 ggcgcatggc gagcgaggac aagtaaataa tcgaaagagg tgggtggggt gttgagcggc
      781 tcatcgttaa gtagcacacg gcgggtgtgt aggtctaaca ccagtttgcc gcgtttgagg
      841 aaacgttcct tctcaaaaga aaccggcgcc ataggttggg ttttgcctgg ttcgccgcca
      901 tctaattctt ttaactcgtt ttgtaaggaa gcaatttgga gcataatttc tctgcgccgc
      961 ttttcttgtt gttgcttttc taagatattg cgtgctcgct caatgatggc ttcaggtttg
     1021 atgggtttta gcaggtaatc tgtagcacca tgacgcaaag cttcgaccgc cgagttcaca
     1081 tcgggctgag ccgtgaagag aataaccggc aacgttgggt gttctgtgtg gatgaccttg
     1141 agagcttcca tgccgtgcat tcctggcatg cgtaggtcgg ttaggatcaa gtcaaattgt
     1201 gtggtgcgca gatagtcaag cccttgttcg gcgccttcag cagtggtcac ttcaaacccg
     1261 gcttgttgca agatgcgagc caaggttttg cggatcgatg cttcatcatc aatgatgaga
     1321 atatgaccgg tgatgttcat tctaactccg ctgaggcagg ttcaattggt aaccaaactt
     1381 tgaaggtcgc gccagtttcg gggttgttct cggcggtgat acgtcctctg tgcttgataa
     1441 caatatcgtg tgagatcgta agcccaatgc ctgtgccgcg ttttttgttt gtaacgaagg
     1501 ggtcaaaaat gtttggcagg atgcttggcg cgataccagg accagtatca gatacagcga
     1561 tcatgatctc ttgtgtctca tttaaaccca ttgtgctgac ggtaagtttg ccaccatctg
     1621 tcattgcctc tacagcattc atgaaaagat ttaatgtaac ttgccgtatc tgtcctggca
     1681 gtgccatgat agctggaata tctgggtctg ggtgaaactc aaatgccaca ctgttcttgc
     1741 gtaggtgtgt agcaagcaag gcatgtacat cttcaataat attgttgaca tgcgctggct
     1801 gtaagtcttc cacttgaggc ggacgatagg tttccctaag gcgttcgatc aggcttgcca
     1861 tgcgttctga ttcagccaat acgatttcaa ggtcatttaa accttgttgt gaaagtccct
     1921 tttcctcttt taggaggaat agggcatttt gtatagcttg cagtgggtta ttcaattcat
     1981 gagaaacgga ggcaagcaac cgtcccatta cggcgagacg ttcgttttgt acaagctgat
     2041 tgcgaacggc tttttcttgc tgcaatgctg tttgcagatt ttcgtatagt tccgttttat
     2101 gtaacgcgac tgcaagttgg tctgctacgg tcaatacaag ttgttggtcg cgttgtgtga
     2161 attgacgtgc agatgtttgt tggatatcca aaatgccata taacttgtct ccattgagta
     2221 cagggattgc catttcagat ttggtgtctg gcaagagggg gttggcaagg tagaacatga
     2281 caccatctac gttgtttgtg aagaagggcg tacccgtttc ggcggcatag cccacaatgc
     2341 cagagccagc gcggatctca tgtttctttt cgaccagaat cttgccaacc gctccgcttg
     2401 cagcttttgt ataaaagttt cccgttgcag ggttgggcac atacacctga acatggtaat
     2461 aatcaaagtt attttttaag aggtttacgg cgtcatttag caactcatca gggtcaagtg
     2521 ctaccatgcc tttattgatg tgatacaaag cacttgtctc ttttagtgcc tgacgggtcg
     2581 tttcgataag caaagcgctt tcaagcgcaa ccgtggtcgc attgaccaaa ggcgtaagac
     2641 ggtgaatcgt ctcctctgaa aacttattcg gcgtattttc atcgatgtat aacaaaccta
     2701 ataattgttt attccacaac agtggaatgc aaagatggga ccggatccat tccagccccg
     2761 gaatggttat ccaattttca tccaaggcgg tatcggcaat gatcgatgtc tttggattct
     2821 tgatcatggt ttgatctaaa ggatacatgc ctgtatcttt gactaagttt ttgaatactt
     2881 cttcgcctcg gttttcgtag ccgcgccatg ctgcagttct cagtatgtta ttttctagca
     2941 gggcaatatt tgcaccacag cccggcagaa gcctttgtac ctggttcaaa atctcgatta
     3001 gaactttatc taactctaca tgcgacacaa gagctaatgt tgcttcggca ctggtttcgg
     3061 cgcgtaagcg ttggtcatgc tctgcttcta aaaggtgttc gcgctcgatt tctgccagct
     3121 ttcggtcagt aatatccata ccgatcgact gatattcggc tttgatgtca ttttcgtttg
     3181 taattaatcg gtctgtccac tgcacccagc gtttttcacc tttgcaattt ttctcgtgga
     3241 tctcatacac tataaaaggt ttatcaggtg tgagagaata aaaattggat cggacaagat
     3301 ctttttgttc ttcgtctaac atatcgaaga aactcttgtt gatgagttca cttggtgtgc
     3361 caccgaagaa atctgcatag gcttggttga cataggtcag cgtaccacct cggtcaaagc
     3421 gaca
//
LOCUS       DAOQJE010000316         3421 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_17759_length_3421_cov_6.207071, whole genome shotgun sequence.
ACCESSION   DAOQJE010000316 DAOQJE010000000
VERSION     DAOQJE010000316.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3421)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3421)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3421
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17759_length_3421_cov_6.207071"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(90..1262)
                     /locus_tag="PLT08_09220"
     CDS             complement(90..1262)
                     /locus_tag="PLT08_09220"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF014069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HD-GYP domain-containing protein"
                     /protein_id="HNE04686.1"
                     /translation="MKALFENLWSRISDLAYIETDNETIQRKGFLLSLYHLLILGILS
                     YTTINTLLYLLFYPSLEYGIYLLEDVVMFGLFYVFWRLTKRGNISLIGHISITLTILL
                     AIVFSDPKYLEYSMVIFALPIAISSFTLRPAMSFAYAFLISFVYILYSFIQSYVWEYN
                     LTPIVALFALAVTTWVISQQLENAISKNEKLVKDQQKTYDELKDAYETTLEGWSKALE
                     IRDQETQGHSKRVSDLTLRIARRMGFNDEQLVHVSRGVLLHDIGKIGIPDEILHKPGP
                     LNEKETRIMRLHPQISVDLLSPIEYLKPALSIPKYHHEKWDGSGYPHGLSGEMIPLEA
                     RIFAIVDVYDALSHDRPYRKAWSKEKAIEHIKSETGKHFDPAVTLIFLEEIEKKKR"
     gene            complement(1297..2157)
                     /gene="prmC"
                     /locus_tag="PLT08_09225"
     CDS             complement(1297..2157)
                     /gene="prmC"
                     /locus_tag="PLT08_09225"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: protein motif:HMM:TIGR00536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HNE04687.1"
                     /translation="MTQTLGDYLIHKIPKLPDDLTQQDAQLLLAHVIGRSRTWLLAHL
                     DAPLSAPLLDSANQAFARLEAGEPLPYILGHWEFFGMDFDITPDVLIPRPETETMVEK
                     AIKWLSASGERRTVADIGTGSGIIATSIAMHVPNANILATDISRAALKVAKHNAEKFN
                     VQHRIDFLECDLLPEHMDPLPTDRHFDLICANLPYIPTQTMRRLPIYGREPTLALDGG
                     EDGLDIYRRLLNVAPNWLAPYGMILLEIEATQGIKALNLATDIFSESSISLYQDFAGH
                     DRLLEIIPRL"
     gene            complement(2154..3005)
                     /locus_tag="PLT08_09230"
     CDS             complement(2154..3005)
                     /locus_tag="PLT08_09230"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HNE04688.1"
                     /translation="MRKISLPRQIATQLIFLLVGLFILIPVWSTFRLAFDGSLRGRPT
                     EFTWLPKEFSAEAFTKVLDRPYQSVEFNILLRNSMIVSIGAAVLAVILGITLAYGFAR
                     FRFPAKQTGLFVLLLAALLPPIAFATPLYIILSLLKIRTTLLGLVIVYAAFAMPFAIW
                     NMRATFAAIPKELEEAAYLDGANHYQTFLNISLPISLPSISVTAMIAFLRGYTEFAIG
                     WLFVDKADNVTLSMAVYALVNGQFSGAQPWSYLGSLAIIMSIPVMVIFIFFQETLMER
                     LLFQETS"
     gene            complement(2998..>3421)
                     /locus_tag="PLT08_09235"
     CDS             complement(2998..>3421)
                     /locus_tag="PLT08_09235"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HNE04689.1"
                     /translation="WFLVLLIPAIWYGTPLIMLASNASLKTIPKEIFEAASLDGANSW
                     QILQHMVLPLIRPVLLPAIIIRGIYAFNQFYLFQAMPFSEGTLANFSYNVFNPSSRFG
                     GGQFAISAVINIITVLILIVLVSAFNRRMQTREEFINA"
BASE COUNT          976 a          675 c          889 g          881 t
ORIGIN      
        1 tatttgggca gtttaatggt tcctgaacct tgattaagga ttggattaga ggattaaggg
       61 aggaaagatt aaattttgtg aagatatttt tatctcttct ttttttcgat ttcttctaaa
      121 aagataagag tcacagccgg gtcgaaatgt ttccctgttt ctgattttat atgctcaatc
      181 gccttttctt ttgaccaagc ttttcggtag ggacggtcat gggaaagcgc atcgtacaca
      241 tccacaatag caaagatgcg agcttctaag ggaatcattt ctcctgatag cccatgggga
      301 tatccgctac cgtcccactt ttcatggtga tatttgggaa tggacaaagc aggtttgaga
      361 tattcaatag gggagagcaa atccacagat atttgcggat gcaacctcat gatgcgagtc
      421 tctttctcgt ttagcggacc tggcttatgc aaaatctcat cgggaattcc gatctttccg
      481 atatcgtgta acaaaacccc tctagataca tgtacaagct gttcatcatt gaatcccata
      541 cggcgagcaa tgcgcagcgt gaggtctgaa acccgtttgg aatgaccttg ggtctcttgg
      601 tctcgaattt caagcgcttt cgaccagcct tctaatgttg tttcataagc atcttttagc
      661 tcatcatatg tcttttgctg atcttttact agtttctcat ttttgctgat tgcattttca
      721 agttgctgtg aaatgaccca ggttgttacc gcaagagcaa acaaagcaac aataggtgtg
      781 aggttgtact cccaaacata gctttggatg aaagaataca ggatgtaaac aaaactaatt
      841 aagaaagcgt atgcaaacga catggcaggt cgtaacgtaa agctggagat tgcaatcggt
      901 aatgcaaaga taaccattga gtattcgaga tattttgggt cactaaatac aatagcaagc
      961 aaaatggtca acgtgatcga aatatgaccg atgagagaaa tgtttcctct tttggttaga
     1021 cgccaaaaaa cataaaacaa cccgaacata accacatctt caagtagata gattccatat
     1081 tcgaggcttg gatagaaaag aagataaagc aaagtgttga tggtggtgta actcaaaatc
     1141 cccaaaatca gtaggtgata taaggaaagc aaaaagccct ttctttgtat ggtctcgtta
     1201 tctgtctcaa tatatgccaa gtctgaaata cgcgaccata ggttttcaaa gagtgctttc
     1261 attaagatat gcctagattt gattatatct tttgttttag aggcgtggaa taatttcgag
     1321 caaacggtca tgacctgcga agtcttggta cagtgaaatt gaagattcag aaaaaatgtc
     1381 tgttgcgagg ttcaaggctt taatgccttg cgttgcttca atttcaagca gaatcatgcc
     1441 atatggggca agccaattcg gtgcaacgtt caaaagacgg cgataaatat ctaaaccatc
     1501 ttcgccacca tcgagagcca gtgtgggttc tcgcccatag atgggaagcc tgcgcatggt
     1561 ttgggtgggg atataaggca ggttggcgca gatcaaatca aaatgacggt cggtgggcag
     1621 cgggtccatg tgttcgggaa gcaagtcaca ttccaaaaaa tcaatgcgat gctggacgtt
     1681 aaacttttcg gcgttatgtt ttgcgacctt gagcgccgca cgagaaatat cggtggctag
     1741 aatgtttgca ttcgggacat gcatggcaat ggatgttgcg ataatgccag aacctgttcc
     1801 aatatcggcg acggttcttc tctcgcccga agcggatagc cacttaatgg ctttttctac
     1861 catcgtttct gtttctggtc tcgggatgag tacatcgggt gtgatgtcga agtccatgcc
     1921 gaagaattcc cagtgaccga ggatgtacgg cagcggttca cccgcttcga gccgggcgaa
     1981 ggcttggttc gctgaatcaa gtagcggcgc ggaaagaggc gcgtcgagat gggcgagcag
     2041 ccatgtgcgt gaacgtccga tcacatgcgc aagcaggagt tgcgcatctt gttgggtcag
     2101 gtcatctggc agttttggaa ttttgtgaat gagataatcg cccagagttt gggtcatgag
     2161 gtttcctgaa agagtaggcg ttccatgagg gtttcttgga aaaagatgaa aatgaccatc
     2221 actggaatgg acatgatgat ggcgagtgag ccaagatagc tccatggttg tgcgccagag
     2281 aattgtccgt tgacgagggc gtagactgcc atggaaagtg tgacgttatc ggctttatct
     2341 acaaagagcc agccaatggc gaattcggtg tatcccctga gaaaggcgat catggcggta
     2401 acagaaatag acggcaatga gatgggcagg ctgatgttga ggaaggtttg gtagtgattc
     2461 gctccgtcga ggtaggcggc ttcttctaat tcttttggaa tggcggcgaa agtggcacgc
     2521 atgttccaaa tggcaaaggg catggcgaac gccgcataga cgatcaccaa gccgagcaaa
     2581 gttgtgcgaa tcttcagcag acttaagatg atatacaacg gagtggcaaa tgcaatgggg
     2641 ggcaacaatg ccgccagcaa taaaacgaac aagccggtct gcttggcagg aaagcgaaaa
     2701 cgtgcaaacc cataggcgag tgtgatgccc aatatcactg caaggactgc cgcaccaatc
     2761 gaaacgatca tactgttgcg aagtaggatg ttgaactcta cggattgata ggggcgatct
     2821 aatactttcg tgaaggcttc agcggagaat tctttgggca gccatgtaaa ctcggtagga
     2881 cgtccgcgca gggagccatc gaaggcgaga cggaaggtgc tccagaccgg gatgaggatg
     2941 aagagaccga ccaacaagaa gatgagttgg gttgcgatct gacgaggcag ggatatttta
     3001 cgcattgata aactcctcgc gagtttgcat gcggcgattg aaggcgctga ccaagacgat
     3061 caggatcaga accgtgatga tgttgataac ggcagagatt gcaaattgac ctccgccaaa
     3121 tctgctggaa ggattgaata cattgtaaga aaagtttgcc agagtacctt cgctgaatgg
     3181 catagcttga aatagataga actgattgaa ggcataaata ccgcgtatga taatggcagg
     3241 taacaaaaca ggtcttatca acggcaagac catatgctgt aagatttgcc aactattggc
     3301 tccatcaaga gaggcggctt caaagatttc tttcggtatg gttttgaggc tcgcatttga
     3361 agcgagcata atcaaaggtg ttccgtacca tatggcagga ataagcaaaa ccaagaacca
     3421 a
//
LOCUS       DAOQJE010000317         3420 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_17771_length_3420_cov_2.801189, whole genome shotgun sequence.
ACCESSION   DAOQJE010000317 DAOQJE010000000
VERSION     DAOQJE010000317.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3420)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3420)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3420
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17771_length_3420_cov_2.801189"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1848)
                     /locus_tag="PLT08_09240"
     CDS             complement(<1..1848)
                     /locus_tag="PLT08_09240"
                     /inference="COORDINATES: protein motif:HMM:NF012602.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HNE04690.1"
                     /translation="MSWIEEIANPQARQWEEFYRNRWQHDRVVRSTHGVNCTGSCSWM
                     IYVKDGIVTWELQAVDYPTLQPGLPPYEPRGCQRGISFSWYQYSPIRLKYPYMRGVLL
                     DLWRKAKEEHKDPVAAWTAIMENEAARRSFQQARGKGGFRRVKWDEALEMISASTIYT
                     VKKYGPDRIIGFSPIPAMSMLSYAGGSRFMQLLGGVSMSFYDWYSDLPPASPEVWGEQ
                     TDVAESADWFNAKFIASVGSNMSMTRTPDVHFAAEARHNGTKLVVFAPDFSQVAKYAD
                     WWVPVNAGQDGAFWMAVNHVIMNEFHYQNPTPYFLDYLRQYSDAPFLVELNEVDGKFV
                     PGRMIRAGQVERTKDVENGEWKFLVWDEISDAPRMPQGSLGFRWQQQKGQWNLEPKDG
                     LDGSEIRAQLTFLGSADQLSVSFAEFGENKSFQRNIPVRYIETASGKTAVATIYDLLM
                     AQYGVGRGLEGDYPANYDDENLSYTPAWQERYTGIDRQTVIQFAREWAVTAVKTEGKC
                     MVIIGAGVNHWYHNNLIYRACIGTLMLTGCVGRNGGGLNHYVGQEKLAPAAPWASIAF
                     ALDWQKPPRQMNTPSFHYVNTDQWRYERTYTEPQPVTRPGDEQHRDMTQE"
     gene            complement(1923..2498)
                     /locus_tag="PLT08_09245"
     CDS             complement(1923..2498)
                     /locus_tag="PLT08_09245"
                     /inference="COORDINATES: protein motif:HMM:NF012264.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HNE04691.1"
                     /translation="MTNKKGKSKGSATLFIVLLFAVVLSGCASAPRKGAIPVSASAIG
                     AGDAENGRKLFMGYAHFENEGPPCMGCHSIGDNGLLGGGNMGPNLTDVSDRLTQDKMV
                     SILANSGAEISPVMEPIYTTHPLTESEQADLIVFMTKSAGEEEVDKEWMVFVLSFGGV
                     GAAVVFFGLVYRSRLRGVRKTLVRDAVKKQQ"
     gene            complement(2715..>3420)
                     /locus_tag="PLT08_09250"
     CDS             complement(2715..>3420)
                     /locus_tag="PLT08_09250"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HNE04692.1"
                     /translation="LNGALGLPYGGRQKEEVNMEQVRVLMVDDHALFREGLAGIIRSQ
                     PDMQVVGEASDGFEALVKAQELKPDLILMDVEMPGIGGIEAARQIRHALPETIIVMLT
                     IRGDDSMLFDALKNGAQGYLLKDIRSQDLLEMLRGALRGEAALSHNLAGRVLSEFRRL
                     STGGVSESEDDGGLSEREQQVLVQAAKGAMDKEIAAQLNISLNTVKTHMRNILAKLHV
                     RTRREAAKAAHVKGIL"
BASE COUNT          745 a         1050 c          842 g          783 t
ORIGIN      
        1 ttcctgagtc atgtcgcggt gttgctcatc gcccgggcgt gtaacaggtt gaggttccgt
       61 gtaggtgcgc tcatatcgcc attgatcggt gttgacataa tggaaggaag gcgtgttcat
      121 ttggcgcggc ggcttctgcc aatccaatgc gaaggcaatc gaagcccacg gcgcggcggg
      181 agcaagtttt tcctgcccga cgtaatgatt caacccgccg ccgttgcgcc ccacacagcc
      241 cgtcaacatc aacgtgccga tacaggcgcg ataaatgaga ttgttgtgat accagtgatt
      301 gacacccgcg ccgatgatga ccatgcactt gccttcggtc ttgacggcgg tcaccgccca
      361 ctcgcgcgca aattgaatca cggtctgtcg gtcaatgcct gtgtagcgtt cctgccatgc
      421 gggcgtgtac gaaagattct catcgtcata gttcgcgggg taatcgcctt cgagtccgcg
      481 ccccacgccg tactgcgcca tcaacagatc gtagatggtc gccacagcgg ttttgccgct
      541 cgcggtttca atatatcgaa cggggatgtt gcgctggaag gatttgttct cgccaaactc
      601 cgcaaacgaa acggacaact gatccgccga acccaggaag gtcagttggg cgcggatctc
      661 gcttccatcc agaccgtcct tcggctccag attccattgc cccttctgct gctgccagcg
      721 gaatccgaga ctgccctgcg gcatccgcgg cgcgtcgctg atctcatccc acaccaaaaa
      781 tttccactcg ccgttttcaa catctttcgt acgttccacc tgccccgcgc ggatcatgcg
      841 cccaggcaca aattttccgt ccacctcatt cagttccaca aggaagggcg cgtcggaata
      901 ttggcgcaga taatctaaaa agtaaggcgt cgggttctgg taatggaact cgttcatgat
      961 gacgtgattg accgccatcc agaacgcgcc atcctgcccc gcgttgacgg gaacccacca
     1021 gtcggcatac ttggcaacct gactaaaatc aggcgcgaag acaaccaact tcgtcccgtt
     1081 gtgacgcgcc tccgcggcga aatgcacatc gggcgtgcgc gtcatgctca tgttcgaccc
     1141 gacggacgcg atgaacttgg cgttgaacca atccgcgctc tccgccacat cggtctgctc
     1201 gccccacact tcggggctgg cgggcggcag gtcgctgtac caatcgtaga aactcatgct
     1261 cacgccgccc agcaattgca tgaagcggct cccgcccgca taactcaaca tggacatggc
     1321 gggaatcggc gagaacccaa taatgcggtc gggtccatat tttttgacgg tgtagatcgt
     1381 ggacgcggag atcatctcca acgcctcatc ccatttcacg cgtcggaatc cgcctttgcc
     1441 gcgcgcctgc tggaacgacc tacgcgccgc ttcattctcc atgatggctg tccacgctgc
     1501 gacagggtct ttgtgctcct ctttggcttt gcgccacaag tcgagcaaca ccccgcgcat
     1561 gtacggatac ttgaggcgga tggggctgta ctgataccac gaaaacgaaa tcccgcgctg
     1621 acaaccgcgc ggttcatacg gcggaaggcc aggctgaagc gtggggtaat ccaccgcctg
     1681 caactcccag gtcacaatgc cgtccttcac atagatcatc cacgaacaac tgcctgtgca
     1741 attcacgcca tgcgtgcttc gtaccacgcg gtcatgctgc cagcgattcc gataaaactc
     1801 ttcccactga cgggcttgcg ggtttgcgat ttcttcgatc cagctcacag atacctccta
     1861 gtttgcattt cagtaccgca aggtttacct tgcgaatgac aatgcgagca ttgtcgtatg
     1921 aattattgtt gcttcttcac cgcatcacgg acaagcgtct tgcgaacgcc gcgcaaacgg
     1981 ctgcggtaga caagcccgaa aaacacgact gccgccccaa ctccgccgaa actaaggaca
     2041 aacaccatcc attccttgtc aacttcctct tcgccagcag acttcgtcat gaagacgatc
     2101 aagtcggctt gctcagattc agtcagcgga tgcgtggtat agatcggctc cataacaggc
     2161 gaaatttccg ctccgctatt ggctaaaatc gagaccatct tatcttgcgt gagacgatca
     2221 gaaacatcag tcaggttcgg acccatattt cccccgccga gcagtccgtt atcgccaata
     2281 ctatggcatc ccatgcaggg cggaccttcg ttctcaaagt gggcgtagcc catgaataat
     2341 tttcgcccgt tctccgcgtc tccggcgcca atggcactgg cagaaaccgg tatcgcccct
     2401 ttccttggcg cggacgcgca acctgataat acaacggcga aaagcaatac gataaaaagg
     2461 gtcgcactcc ctttcgattt gcctttttta ttggtcatgg gttccagcct cctgatgaag
     2521 ggaacaaagc attggacggc gtctcctaat aaagagtttt gagttacttt atttgtatgt
     2581 gaaaaaatga acaatactcc cccaagaata ctgtacttct tgcctcattc ccatcccccg
     2641 ccatcctgac tcaggaatga aaaaagagtg attgatcaat cactcttttt tcaaaagaat
     2701 tagttctccc tggtctacaa gatccctttg acgtgggctg cttttgccgc ctcgcgccgc
     2761 gtgcggacgt gcagtttggc gaggatgttc cgcatgtgag tcttcaccgt gttcaggctg
     2821 atgttcaatt gggcggcgat ctctttatcc attgcgcctt ttgccgcctg caccaacacc
     2881 tgctgttccc tttctgacaa cccgccatcg tcttccgact ctgaaacgcc gcccgtgctc
     2941 agacgccgaa actccgaaag gacgcgcccc gccagattat gcgacaatgc ggcttcccca
     3001 cgcaatgcgc cgcgcagcat ttccaataaa tcctgcgagc ggatgtcttt taacaaatag
     3061 ccctgcgctc cattcttgag cgcatcgaaa agcatggaat cgtcgccgcg aatggtcaac
     3121 atgacgatga tagtctctgg cagggcatgg cgaatctgcc gcgccgcttc aataccgcct
     3181 atgcctggca tttccacatc catgaggatc aaatctggct ttaattcctg cgccttgacg
     3241 agggcttcaa aaccgtcgct ggcttcgccg accacctgca tatcgggctg ggacctgata
     3301 atacccgcca gtccctcgcg gaaaagcgca tgatcgtcaa ccatcaatac gcggacttgt
     3361 tccatgttca cctcttcctt ctgccttcca ccataaggta acccgagcgc cccgttcagg
//
LOCUS       DAOQJE010000318         3419 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_17774_length_3419_cov_5.020511, whole genome shotgun sequence.
ACCESSION   DAOQJE010000318 DAOQJE010000000
VERSION     DAOQJE010000318.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3419)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3419)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3419
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17774_length_3419_cov_5.020511"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1051
                     /gene="mutY"
                     /locus_tag="PLT08_09255"
     CDS             <1..1051
                     /gene="mutY"
                     /locus_tag="PLT08_09255"
                     /inference="COORDINATES: protein motif:HMM:TIGR01084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HNE04693.1"
                     /translation="TLSLMKNRLATQLLNWYHAQKRILPWRDHPDPYAVWVSEIMLQQ
                     TRVDTVIPYFEKWMKLFPTVTALASASEQEVLNAWEGLGYYSRARNLHKAAKVVVEKF
                     NGELPRDLEDLRSLPGIGRYTVGAIASMAFGMDEPTLDGNLRRVFSRLFDVDEFADSP
                     VGEKILWELAAQNLPEGEAGDYNQALMDLGATICLPKNPRCLLCPLMDLCKARENGTQ
                     ELRPVLKPKKAVPSYVHVAAVIIERGRVLLSQRPAEGLLGGMWEFPNARIEGDPAKAL
                     VKGLKAATQIQVKKKEALQVVEHAYSHFKVTVYPFVCEKVSIPKNKSLKWVKLTELEE
                     YPMGKVDRQIARQIK"
     gene            complement(1077..2720)
                     /locus_tag="PLT08_09260"
     CDS             complement(1077..2720)
                     /locus_tag="PLT08_09260"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - ribonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HNE04694.1"
                     /translation="MSKKNKLRIIPLGGLGEVGKNMMAYEYGNDIIVVDAGIMFPKND
                     MLGVDYIIPDYEYLKNKSNRILGLFITHGHEDHIGAIQHFIEDIPVPVYATPLTRGLI
                     EAKLHRNGAQHKAQLKTINAGDATRVGPFTLEYFHVNHSIPDAVGIGITTPAGLVVHM
                     SDFKFDQTPVDGWPTDFAKLAEFASRGVDLLLSDSTNAERPGWTPSEAVIQPAFEKVF
                     AEAKGRVIVATFASLISRVQQVADAAVKYGRKMALAGPSMVDNVKIARKMKYLEIPDE
                     IIVPIEQALQMQNHKVVIMCTGSQGEPSSIVGRLSAGTNRQFDLKEGDTVVLSSHPIP
                     GNEETISKTINRLLRRGAKVIHDGIAPIHVSGHAAQEEQKLLINIVRPKHFMPIHGEL
                     RQLMRHAELAQQLGVDPENTIVAENGQVIELVGKKILRGERIPGDYVFVTGESIGDID
                     HDVMRERSQLARNGIMLIDISIDKKTSRLLHEPEIITRGFVSPEDAETLLPDVRKLVL
                     RMVHDDGFDDEKDIINAVKSYLHQQTKRRPMVFVTLSKS"
     gene            complement(3026..3352)
                     /locus_tag="PLT08_09265"
     CDS             complement(3026..3352)
                     /locus_tag="PLT08_09265"
                     /inference="COORDINATES: protein motif:HMM:NF020908.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1992 domain-containing protein"
                     /protein_id="HNE04695.1"
                     /translation="MSFEKAVETIIREAQERGDFDNLKGKGQPLDLDAYFETPEDLRL
                     AYSALKNAGLVSPEVELLQEIAALKERLTTTYEEAQRNKIKKIIHDKQLEFNILLERQ
                     KRHRKG"
BASE COUNT          805 a          806 c          930 g          878 t
ORIGIN      
        1 aaccctgtcc ctgatgaaga accgcctcgc gactcaactc ctaaactggt accacgccca
       61 aaagcggatt ctcccctggc gcgaccaccc cgatccatac gcggtgtggg tttccgagat
      121 catgcttcaa caaacgcggg tggacacagt cattccatat ttcgaaaaat ggatgaagtt
      181 atttcccacc gtaacagcgc ttgcttctgc cagcgagcag gaggtgctca atgcatggga
      241 aggcttgggc tattacagcc gtgcgcggaa tttgcacaaa gccgctaaag tggttgtaga
      301 gaaattcaac ggcgaacttc cacgtgattt agaagatttg cgtagcctgc cggggattgg
      361 tcgctatact gttggggcga ttgcatccat ggcatttggc atggacgaac ccacacttga
      421 tggtaatttg cgacgggtgt tctctcgatt atttgatgta gatgaatttg cagattcacc
      481 tgtcggcgaa aaaattctct gggagttggc ggcgcagaat ttgcccgaag gtgaagcagg
      541 cgactacaac caagccctga tggacctcgg cgcgacgatc tgcctgccga agaatccgcg
      601 ctgtttgctt tgcccattga tggacttatg caaagcacgt gaaaacggaa cgcaggaatt
      661 gcgccctgta ttgaagccga agaaggcagt gccatcctat gttcatgtgg cggcggtgat
      721 tatagagcgc gggcgcgtgt tgctcagtca acgcccggcg gagggattgt tggggggcat
      781 gtgggaattc ccgaacgcaa gaatagaggg cgatccggca aaggcgttgg tgaaaggctt
      841 gaaggcagcg acccagatac aggtcaagaa aaaagaggcg ttgcaagtcg tcgaacatgc
      901 gtacagccac tttaaagtca cagtctatcc gtttgtgtgc gagaaggttt ccatccccaa
      961 aaataaaagt ttgaagtggg tgaagctgac cgagttggaa gaatacccga tggggaaggt
     1021 agatcggcag attgcccggc aaataaaatg accgccttac gacggtcatt tttgttttaa
     1081 gacttactga gtgtcacgaa gaccatgggt ctgcgtttgg tttgctgatg taagtaactc
     1141 ttcacggcgt tgatgatgtc tttttcatcg tcgaagccgt cgtcgtgaac catgcgcagg
     1201 acgagcttgc gcacatcggg caggagggtt tcggcatctt ccggcgagac gaagccgcgg
     1261 gtgatgatct ccggctcatg caggaggcga ctggttttct tatcgatgct gatatcaatg
     1321 agcatgatgc cattgcgggc gagttgactg cgttcacgca tcacatcatg gtcgatatca
     1381 ccaatcgatt cgcctgtgac gaagacataa tctccgggga tgcgttcgcc gcgtagaatc
     1441 tttttaccga ctagttcaat gacctgacca ttttctgcga caatcgtgtt ctcgggatct
     1501 acacccaatt gctgggcaag ttcagcatga cgcatcaact ggcgtaactc gccgtggatg
     1561 ggcatgaagt gtttgggacg cacaatgttg atgagcaatt tctgctcttc ttgagccgcg
     1621 tgcccagaaa catggatggg agcaatgcca tcgtgaatga ctttcgcgcc gcgtctcaac
     1681 agacgattga tggtcttgct gatggtctct tcgttgccag ggatggggtg tgaagaaagt
     1741 acgactgtat cgccttcttt gagatcaaac tggcggttgg tgcctgcgga aagacgccca
     1801 acaatggaag agggttcgcc ttgcgagcca gtacacatga tgaccacttt gtggttttgc
     1861 atctgtaatg cctgctcgat gggaacaatg atttcatcgg ggatttcaag atatttcatc
     1921 ttgcgggcaa tcttgacgtt atcaaccatg ctcggacctg ctaacgccat tttgcgtccg
     1981 tacttgacgg cggcgtctgc gacctgttgt actcgcgaga tgagcgaagc aaaggtggca
     2041 acgatcacac gccctttcgc ttcggcgaag accttttcga aagcgggctg aatgaccgct
     2101 tcagagggag tccaaccggg gcgttcggcg ttggttgagt cggagagtaa caggtccaca
     2161 ccgcggctgg cgaactcagc gagtttggca aagtcggtgg gccaaccgtc gacgggggtt
     2221 tgatcgaact tgaaatcgct catgtgaacg acgagtcccg ccggggtggt gatgccgatc
     2281 ccaacagcat cagggatcga gtgattgacg tggaaatatt ccaaggtgaa aggaccaacg
     2341 cgggtggcgt caccagcatt gatggttttt agttgagctt tgtgttgcgc cccattgcgg
     2401 tgcaatttgg cttcgatcaa cccgcgggtg agcggggtgg catagactgg gaccggaata
     2461 tcttcaataa agtgttgtat cgcgccgatg tggtcttcat gcccgtgggt gataaataac
     2521 cctaaaatac ggttggattt gttctttaaa tactcatagt ctgggatgat gtaatctacg
     2581 cccaacatat cgttctttgg gaacatgatg ccagcgtcga ccacgatgat gtcgttgccg
     2641 tactcatacg ccatcatgtt ctttcccact tcgcccagcc ccccgagcgg gataattctt
     2701 aatttatttt ttttactcat ataatttttc tgtatacctc acagatttca aagattagaa
     2761 accgcctatg cggttaaagt caaggtaggt gttgggtagg agtttcttgc aagaaaggct
     2821 tgcagattcc agtaaacgtc ttttccctca aacaaacaaa aagaccttcg ctgacttgct
     2881 cgcgaagatc gatgccggta aaacacgatt gttctcaaac aaactgccga attcggcagg
     2941 aaaagtcaac ttaactgtca aagagtatag cagggcgggc gggttttgtc aaagcgggcg
     3001 tgttatagaa tcgttaaagc tttggctacc ctttgcggtg ccgtttctgg cgttccagca
     3061 gaatgttgaa ctccaactgc ttgtcgtgaa tgatcttttt gatcttattt cgttgggctt
     3121 cttcgtacgt agtcgtcagc cgttctttga gagcggcgat ctcttgcaat agttccactt
     3181 ctggcgagac caagccggca ttctttaatg ccgaatacgc caggcgtaag tcttcggggg
     3241 tctcaaagta agcatccagg tcaagaggct gccccttgcc tttgaggttg tcaaagtcgc
     3301 cacgttcctg cgcttcacga atgatggttt ccactgcttt ttcgaaactc atattgcgcc
     3361 attatatacc ttatttcaca aagaaaattg acaatctccc tcgtctcttg tacacttct
//
LOCUS       DAOQJE010000319         3418 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_17785_length_3418_cov_5.992566, whole genome shotgun sequence.
ACCESSION   DAOQJE010000319 DAOQJE010000000
VERSION     DAOQJE010000319.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3418)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3418)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3418
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17785_length_3418_cov_5.992566"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..244)
                     /locus_tag="PLT08_09270"
     CDS             complement(<1..244)
                     /locus_tag="PLT08_09270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE04696.1"
                     /translation="MSELVNSIRERYLSALDTIASAANRSGRSPEAVKLVVVTKFQPL
                     EVVQATIEAGAVILGENYAEEGVTKIQSLQNLSAVEW"
     gene            complement(274..1053)
                     /gene="pgeF"
                     /locus_tag="PLT08_09275"
     CDS             complement(274..1053)
                     /gene="pgeF"
                     /locus_tag="PLT08_09275"
                     /inference="COORDINATES: protein motif:HMM:NF014619.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan editing factor PgeF"
                     /protein_id="HNE04697.1"
                     /translation="MPFDQSDGLRYYQFDIFSKNILNAVFTRQGGISPEPWTSLNLSI
                     SVGDDPARVAENRVHAFNALGRNPASIHDAWLIHGTDVIFADAPRSLDAPTQKADILF
                     TDNPEVSLFMRFADCVPLLFHDPKKNVIGISHAGWMGTVKGVAEVSVQAMQERYGSNP
                     QDIVVGIGPSISADHYEVGEDVAAQFREKYGKDSEQVVQVRDEKIYLDLWAANALQLQ
                     KMGVEHIQISGLCTACHLDDWYSHRAEKGKTGRFGVLLAMV"
     gene            complement(1053..2090)
                     /locus_tag="PLT08_09280"
     CDS             complement(1053..2090)
                     /locus_tag="PLT08_09280"
                     /inference="COORDINATES: protein motif:HMM:NF021384.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2085 domain-containing protein"
                     /protein_id="HNE04698.1"
                     /translation="MKPAKGLSHMLNVTLFTKKDCKLCDEVKADLKALQEQYPHRLAE
                     VDIESDAALYEKFRNVIPVIEVGPYTVQAPISRQKLQMTLGAASDRKNQLEKLDDPVY
                     KYKMEKGKKLSTGDRVSFWISKRYLLLLNLFMLLYVGLPFLAPTLMKLGAEAPAQLIY
                     RIYKPLCHQFAFRSFFLFGEQPFYPLAEANLTGYKTFEQATGIAGVDDPYNMARFEAR
                     NYIGDSTIGYKVALCERDVAIYLAILAFGIIFGLTGRRFKSVHWLVWILIGIAPIGLD
                     GFSQLFSQFNWDWLNTLLPYRESTPFLRVLTGALFGLFTAWFAYPNIEESMNETRQYF
                     LKKSAVIEAAE"
     gene            complement(2148..2879)
                     /locus_tag="PLT08_09285"
     CDS             complement(2148..2879)
                     /locus_tag="PLT08_09285"
                     /inference="COORDINATES: protein motif:HMM:NF014713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcdA"
                     /protein_id="HNE04699.1"
                     /translation="MEISQITVWLALLAGLASFLSPCVFSLVPAYVGYLGGIAAGSGT
                     PGEKPNRWLTFSHGVAFVLGFSIVFVLLGVAASFAGGLLYDLRYWLAKIGGVVVIIFG
                     LHMIGVFHIPFLAYDTRVQKAPDPKLGYFSSALMGVFFSAGWSPCVGPVLGAILTLAI
                     NGGSISQGATLLTFYSAGLAIPFLLAALGIGWVTTILRKYNKTMRYVEIAMGVILIII
                     GIMLFAGTFELIAQRGQFFFIDFGL"
     gene            complement(2889..>3418)
                     /locus_tag="PLT08_09290"
     CDS             complement(2889..>3418)
                     /locus_tag="PLT08_09290"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015679.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_function: GO:0071972 - peptidoglycan L,D-transpeptidase
                     activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HNE04700.1"
                     /translation="WADGAGLKILTDADISTINPEVDPNEKTISIDLDYQTLSCYEGA
                     REVYFCRISSGKKYDPVTGQVTDAYATPAGSLLTYWKIISKNMTAGNEGSGYSTPAVP
                     WCTFIASGGVAIHGAFWHNAFGERRSHGCVNVTPEDSKWIFRWTMPYVSLAAGEERRE
                     LPDHGTTVTSTETQF"
BASE COUNT          959 a          845 c          845 g          769 t
ORIGIN      
        1 gccattctac cgcagaaaga ttttgcaaag attgaatttt cgtgacaccc tcttcggcat
       61 aattttcgcc caagatgacc gcccctgcct caatcgtcgc ctgaacgacc tccagcggct
      121 ggaatttggt gacgacgacc aacttcaccg cctccggact tcgccccgac ctgttcgccg
      181 ctgacgcgat cgtatccaac gccgacagat accgctctcg tatagaattg accaattcgc
      241 tcatatgatt attttaaccc acccagggtt ttctcaaacc attgccaaca acacgccaaa
      301 acgacctgtt ttcccttttt cggcacggtg tgaataccaa tcgtctaaat gacaggcagt
      361 gcataagccc gatatctgaa tatgctccac acccatcttc tgcaattgca aagcattcgc
      421 tgcccacaga tcaagataaa tcttctcatc ccgcacctga acgacttgtt cagagtcttt
      481 gccatatttc tcacggaatt gcgcagcaac gtcctcacct acctcataat gatctgcact
      541 aatagacgga ccaatgccga ccacaatatc ttgcggattg gaaccgtatc gttcttgcat
      601 ggcttgcaca gagacctcag cgactccttt cacagttccc atccaacctg cgtgtgaaat
      661 gccgattacg tttttcttcg ggtcgtgaaa aagcaaaggg acacaatctg caaagcgcat
      721 gaagagcgaa acttcagggt tgtcggtaaa aaggatatct gctttttgag tcggagcatc
      781 gagcgagcgc ggggcatccg cgaagataac gtctgtgccg tggatcagcc atgcgtcgtg
      841 gatagaggcg gggtttcgtc caagcgcatt aaaagcatgg actcggttct cagccacgcg
      901 cgcgggatca tctccaaccg aaatgcttaa gttgagagaa gtccacggtt cggggctaat
      961 gccgccctga cgagtaaaca ccgcattgag gatattcttt gaaaagatat cgaactggta
     1021 ataacgcagc ccgtctgatt ggtcaaaggg cattattcag ccgcctcgat cactgctgac
     1081 tttttgagga aatactgacg ggtttcgttc atggattctt cgatgttggg ataagcaaac
     1141 caagccgtaa agagaccaaa gagcgcgccg gtaagaacac gtaagaacgg tgtactttca
     1201 cgatatggta gaagtgtatt gagccaatcc caattgaact gactgaagag ttgcgaaaag
     1261 ccgtctaacc cgatgggcgc aatgccgatc aaaatccaca ccagccaatg tacagacttg
     1321 aagcggcgac cggtgagtcc aaagatgatg ccgaatgcca gaatagcaag atagatcgct
     1381 acatcgcgtt cgcaaagcgc cactttgtaa ccaatggttg aatcaccaat atagttgcgg
     1441 gcttcgaagc gagccatatt gtatggatca tctacacctg caatgccggt ggcttgctca
     1501 aaggttttat agcctgtgag gttcgcctct gccagcggat aaaaaggctg ttcgccaaac
     1561 aagaagaaag aacgaaaagc aaattgatgg caaagaggtt tatagatgcg atagatcagt
     1621 tgcgcgggtg cttcggcgcc caacttcatg agggttggcg caagaaaagg cagccctaca
     1681 tacaacaaca tgaacagatt caacaaaagc aaataacgct ttgatatcca gaatgaaaca
     1741 cggtctccag tagacaattt ctttcctttc tccattttgt atttataaac ggggtcgtcc
     1801 aatttttcga gctgattttt acggtcggag gctgcaccca gagtcatttg tagtttctgg
     1861 cgcgagatgg gcgcttgaac ggtgtagggt ccgacttcga taacggggat aacatttcta
     1921 aatttttcgt acaacgccgc atctgattca atgtccactt ctgccagacg gtgcggatat
     1981 tgttcttgca gcgccttcaa gtcggctttg acttcatcac agagcttaca gtcttttttg
     2041 gtgaagagag tgacattgag catatgcgaa agtccctttg caggtttcaa aataattccc
     2101 gcaggaatgg ctacctgcgg gagtaaaaac gaaatatgtc ttggggatta tagaccgaaa
     2161 tcaatgaaga agaactgtcc gcgttgggcg atgagttcga acgtccctgc aaagagcatg
     2221 ataccgataa tgatcaggat gacgcccatg gcgatctcaa catagcgcat ggtcttgttg
     2281 tatttgcgga gaatggtggt gacccaacca atgcccaagg cggcgagaag gaatgggatg
     2341 gcaagacccg ccgaatagaa agtaagaagc gtggcgccct gagagatcga gccgccgtta
     2401 atggcgagag tcaaaatcgc accgaggaca ggtcctacac agggagacca tccggcagag
     2461 aagaatacgc ccatcaatgc ggacgaaaaa tatccgagct tggggtctgg tgctttttga
     2521 acacgggtgt catacgcaag gaacggaatg tgaaagacac cgatcatgtg caaaccgaag
     2581 atgatgacga ccacgccacc gatcttcgcc aaccaatagc gtaaatcgta taacaaaccg
     2641 cctgcaaagg aagccgctac accgagcaac acgaatacga tactgaaccc tagtacgaaa
     2701 gcaactccat gtgaaaaggt aagccaacga ttgggctttt cacccggagt tccactgcct
     2761 gctgcaatgc cgccaaggta tcccacatag gctgggacaa gagaaaatac acaaggggaa
     2821 aggaaagaag ccaacccggc aagcaaagca agccaaacag taatttggga aatttccatg
     2881 gacgcctctt agaattgggt ttcggtagaa gtgactgtgg tgccgtggtc tggcagttcg
     2941 cggcgttctt cgccagcggc aagagaaacg tatggcatcg tccaacggaa aatccacttg
     3001 gaatcttcag gcgttacatt gacacagcca tgcgagcgac gttcaccaaa ggcgttatgc
     3061 cagaaggcac catgaattgc cacgccgcca gaggcaataa acgtacacca aggcactgcc
     3121 ggggtggaat agccagaacc ttcattacct gccgtcatgt tcttggagat aattttccaa
     3181 taggtcagca aggaaccagc cggggtggca tatgcatcag tgacctgccc tgtgacgggg
     3241 tcatatttct tgccagaaga aattcgacag aaatacacct cacgggcgcc ttcataacag
     3301 gaaagagttt gatagtccaa gtcgatggag atggtctttt cgttcgggtc cacttcgggg
     3361 ttaatggtgg aaatatcagc atcggtgaga atcttcaacc cagcaccatc tgcccaat
//
LOCUS       DAOQJE010000320         3409 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_17854_length_3409_cov_3.869112, whole genome shotgun sequence.
ACCESSION   DAOQJE010000320 DAOQJE010000000
VERSION     DAOQJE010000320.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3409)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3409)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3409
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17854_length_3409_cov_3.869112"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(193..2001)
                     /locus_tag="PLT08_09295"
     CDS             complement(193..2001)
                     /locus_tag="PLT08_09295"
                     /inference="COORDINATES: protein motif:HMM:NF012464.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HNE04701.1"
                     /translation="MTNNFPFERGDILSLISEEVAKGRPDNVWAVYSRKSRVKPNDPG
                     YSMEYQPETAEKYARDHGAKEVLFFQDVDRSGKNSKREGFQDLLRAVKSGRVDVVLVH
                     RIDRIFRNHRSTLELIDLLKKHKVIFISVTENIDTSTWWGRLVLAVLSSLAEAYILQT
                     SDRNREIFDSVRSHGKQLGRHPLGYCNGLCSTCKDFNGNGYCPLYGMPDRPESLRGTL
                     MVPHPMDRHAVVLIYNLYKQGWSFRDIADYLNKSLVALPDGTQVKFRPRGLYGVKRRE
                     GEQKFSRESIRVILSNPDYAGYIPERRRPQLSMEDDLEHPENIPNAKIDGNAREILSL
                     HPANHESLIPYSLWLEVQSLKKTRATAPTAISGKPARKYPLSGVARCWECYETLEKEY
                     TLRGSTGGRGHIYYRCAYTQDIAPSRKKNRKTPRVEGVNTMINVADQQLVARHKQIPA
                     RKLEEQIDSLMAHLVIPREWEDMIAAYFLDDDGMAAVDRAGHTFQRDLQKLKDQYEAG
                     LISGPELERKSRLLQGSLKELMPSSKPESKEILKELRPFGIFWQQLQDGDKHILLGIV
                     FDSLYLNHEGKLIRALANEPFDRLLNLPSGGLMVDE"
     gene            complement(1991..2158)
                     /locus_tag="PLT08_09300"
     CDS             complement(1991..2158)
                     /locus_tag="PLT08_09300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE04702.1"
                     /translation="MSTTTKKPSEQKRIQDFTRELGRILRTIADTVGKPKPEKKPVQT
                     TTNKEQPANDQ"
     gene            2265..2486
                     /locus_tag="PLT08_09305"
     CDS             2265..2486
                     /locus_tag="PLT08_09305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE04703.1"
                     /translation="MKAITSQVKIIKVKILKNEHSIMINKLFSIKSENVRKLIENKMC
                     YMDYFMRNKLNSYSSEKPKPKESKNQQGN"
     gene            2497..3300
                     /locus_tag="PLT08_09310"
     CDS             2497..3300
                     /locus_tag="PLT08_09310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE04704.1"
                     /translation="MSQLSIKNWGTQFEKAILQGYESRKAFVHEINEYFRKKHEGHLK
                     ILEDFKQAQEQKKTISSEIYENDLVKKYRSGQKISTPSQWIWDTEITKWVNGKKSPTN
                     SKTLIPILEFFFEKNIMSKLEANEWIMNLKFVMPDKELHKIIKYIGANTSSFTTNPWI
                     LDKNKIQEFMYIGTLFVVYGPVGCGKSNFIEHVTNYLHHHFRSEISKGVFLVNGKNMS
                     VGDALFHIMSNVLECLPRSPPGREGMVHGYSVPLWTRFGRRRFPPRRTL"
BASE COUNT          971 a          770 c          723 g          945 t
ORIGIN      
        1 tgaaggtctt tgatatcaag caagtgagcg ctgaacgcct cccccccgag gcagttgatg
       61 ttgcactgga tattgccagg attcccgacc caggcacccg caaacaagta gcggaagcgg
      121 tgcgggtatt actaaaaaaa tattttggcg attaacgaaa aagaacagac tgtgtaagcc
      181 tgttcttttt ttctattcat ccaccatcaa accacccgat ggcaagttaa gtaaacgatc
      241 aaacggctca tttgcgagtg ctcggattaa tttgccttca tgattgagat ataggctgtc
      301 aaaaacgata cccagcaaga tatgtttgtc accatcctga agttgctgcc aaaatatacc
      361 aaacggacga agttctttga gaatttcttt actttctggt ttggatgagg gcatcaattc
      421 tttaagcgag ccctgcagca gtcgcgattt ccgttcaagt tccggacccg agataagccc
      481 tgcttcatat tggtctttga gtttctgcaa atctctttga aaggtatgcc ccgctcgatc
      541 aaccgccgcc atgccatcgt catcaagaaa ataagcggca atcatatctt cccattcgcg
      601 cgggattacc aagtgagcca tcaaagaatc aatctgctct tcaagcttcc tggcagggat
      661 ctgcttatga cgggctacca attgttggtc tgccacattt atcattgtat tcacaccttc
      721 aactcgtggt gtttttctat ttttctttct gctgggcgct atatcctggg tgtacgcaca
      781 acgataatag atatgtcccc gcccaccggt cgaacctcgt aatgtgtatt ctttttcaag
      841 ggtttcatag cattcccaac aacgggcaac accactaagt ggatacttac gggcaggctt
      901 tcctgaaatc gcagttggag ctgttgcgcg tgtctttttg agcgactgaa cttcgagcca
      961 caaggagtac gggatcaaag attcatgatt agctggatga agacttagta tctcgcgggc
     1021 atttccatct attttggcgt tgggaatatt ttctggatgc tccagatcat cttccatact
     1081 caattgcgga cgacgccttt ctggaatata gccagcatag tctggattag agagaatcac
     1141 ccggatactt tcgcggctga atttctgttc tccttcccta cgcttcaccc cataaagccc
     1201 tcgggggcga aatttcactt gagttccatc cggcaaggct accaatgact tgttcagata
     1261 atccgcaatg tcacgaaagc tccatccctg cttatagaga ttgtagatca acacgaccgc
     1321 gtggcgatcc attgggtgtg gaaccatcaa agtaccgcgt agactctcgg gacgatcggg
     1381 catgccatat agcgggcaat acccatttcc attaaaatct ttgcaagttg aacacaaacc
     1441 gttacagtat ccaagcgggt gtctgcctaa ttgtttgcca tgtgaccgca cagaatcaaa
     1501 gatctccctg ttacgatcgc tggtttgtaa aatataggct tcagccagac tcgaaaggac
     1561 tgccagcacc aatcgtcccc accaagtgct cgtatcaata ttctctgtta ctgaaataaa
     1621 tatgactttg tgtttcttca acaggtcgat caattccaat gttgatctat gattacggaa
     1681 gatgcggtct atacgatgca ccagaacaac atccacccga cctgacttaa ccgcgcgaag
     1741 cagatcctga aacccctcgc gcttactatt cttaccagat ctgtccacgt cttgaaagaa
     1801 aagtacttcc ttggctccat gatctctggc gtacttttcc gccgtttccg gttggtattc
     1861 cattgaatat ccaggatcat taggcttaac tcgtgatttt cgcgagtaga ccgcccagac
     1921 attgtctgga cggccttttg ccacctcttc cgaaataagg ctgaggatat cgcctctttc
     1981 gaaggggaaa ttattggtca tttgcgggct gctccttatt cgttgttgtc tgcaccggct
     2041 ttttttccgg cttgggtttg cctacggtat ccgcaatagt gcgaagtata cgccctaatt
     2101 cacgggtgaa gtcttgaatg cgcttctgct cggagggctt ttttgtggtg gtgctcatta
     2161 ttgttttctc ttttctaagt ttatgatctg ccactagtga gctaattcaa ttgaatatgc
     2221 gattacttta ttgctcaaaa gcacaatcgc acagaatgca ataattgaaa gcgattacct
     2281 cacaggtgaa aatcatcaaa gtcaaaatac ttaaaaatga gcattcaata atgattaata
     2341 aacttttttc aatcaaaagt gaaaatgtga gaaaattaat tgaaaacaaa atgtgctata
     2401 tggattattt catgagaaat aaattaaatt catatagttc agaaaaacca aaacccaaag
     2461 aaagtaaaaa tcaacaagga aattaatgtt tcgcctatgt cacaactatc cattaagaac
     2521 tggggaactc aatttgaaaa agcaatattg caaggttacg aaagtagaaa agcttttgtc
     2581 catgagataa atgagtattt cagaaaaaaa catgaggggc acttaaaaat tcttgaagat
     2641 ttcaagcagg ctcaagaaca aaaaaagaca atttctagtg aaatctatga aaatgacttg
     2701 gtcaagaaat atagatctgg acaaaaaatc tccactccca gccaatggat ttgggatacg
     2761 gaaattacaa aatgggtcaa tgggaaaaaa tctcccacca actccaaaac cctaatcccc
     2821 attcttgagt ttttttttga aaaaaatatt atgtcgaaac ttgaagctaa tgaatggatt
     2881 atgaacttaa aattcgtaat gccagacaaa gagcttcata aaattattaa gtacatcggc
     2941 gcgaacacga gttcttttac aacaaatcca tggattttgg acaaaaacaa aattcaggag
     3001 tttatgtata taggcacttt gtttgtggtg tatggaccag tgggatgtgg aaaatcaaat
     3061 tttattgaac atgtcacaaa ttacttacat catcatttta ggagtgaaat atcaaagggt
     3121 gtttttctgg taaatggcaa aaacatgagc gtgggagacg cattattcca cattatgtcc
     3181 aatgttctag aatgcttacc ccggtctccg ccgggacggg agggaatggt ccatggttac
     3241 agtgtcccgc tttggacgcg attcgggcgc aggcgatttc cgccccgccg aactctctga
     3301 aaacgccgtg gtcgtcatgg agcggcgcat catggcccgc gacgcgaacg gcgacgccct
     3361 cgaaacgccg gaccagtgct tccggcgcgt cgcgcgcaac ctggccgag
//
LOCUS       DAOQJE010000321         3402 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_17924_length_3402_cov_5.121004, whole genome shotgun sequence.
ACCESSION   DAOQJE010000321 DAOQJE010000000
VERSION     DAOQJE010000321.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3402)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3402)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3402
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17924_length_3402_cov_5.121004"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(167..922)
                     /locus_tag="PLT08_09315"
     CDS             complement(167..922)
                     /locus_tag="PLT08_09315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE04705.1"
                     /translation="MQNFTGNIGRTVTNRIGGAVAAIGAAIFMALCGALMAFVLAPQQ
                     AIKAANIEKMPQMDAAYVSAAAPGTDILITGYLNGSAPNPQLPKFIAYEAEQWDVSEN
                     KNTDGTPQAPSGSWEHVETIVPDLTLDMSGTPVNILSANNATLSGSNLHEQLVTKSSA
                     FTADYKGQKLGEGSLRYTGFFSGDLVTVLGKKSSGDGVVPEELFAGDRVAFEQYQKDS
                     AKALLFMGFAAMICSPFVGIGGVIAALLGRGRR"
     gene            1075..3183
                     /locus_tag="PLT08_09320"
     CDS             1075..3183
                     /locus_tag="PLT08_09320"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HNE04706.1"
                     /translation="MADWIGQTIGKVRVEKYLARGGMAEVYLGTHLTLDRPVAVKVMH
                     SFVESEPELINRFQREAKAVAALRHPNIVQVYDFDTHDGHPYLVMEYLKGPSLASLLR
                     ELHKNGARLSLSQIAQLLKSLTAGIDYAHSQGIVHRDIKPANIILNNKSGDYTIDQLP
                     RDTECIITDFGLVRLTNSTTQTASGVVSGTPAYMSPEQAQGSVVDSRSDTYSLGIVLY
                     EMLAGRLPFDGDSTLGVILKHISEPPPPIEDISPEIQAVVNKALEKLPENRYQTARDL
                     LMDFYNAVGMHAEAETLYSLRLRTPPPTAALSNKPAPKPNRFLWIGAGILACLCLGVI
                     ATGAVGFSIYSLFPKATTTPQILTDAATPDHMPHGNTAEPGSTPQPISFGEFFGVLRF
                     QGNMDQVTVTASLPDPAEGTQYEAWLIDDGGELLISLGILEKNNSGQYNLTFTDPESR
                     NLLDGFNRMEITLETNPDASPNPSDTILYSSGIPAGALTHVRHLLVSFPSNPNQTPMT
                     GGLIGTSSLINEAGTALEKAFEKGDTTAIRLQAETLINLVVGKQSNEYKDWDKSGKIS
                     DPSDGFGLLLNGGQTGYIEGTITHAELSASAGDATANIKLHSEHVVISAKNVEGWATQ
                     LRDVAKRILESDQVSESDIRLAVSLAEQIYQGVDIDGNESVDPVEGEGGALTAYQHAE
                     YMADMQVLEGANLFPPPGNP"
BASE COUNT          892 a          926 c          782 g          802 t
ORIGIN      
        1 ttgaaggctt gcaatggaaa cccagcgact tgaagccggg cgcgaagttg aatcaacccg
       61 gtccgctgtt caagaagttg gaaccaacag tcattgatga agaacgtgga aggttaggaa
      121 attaacaaaa cgggcggact gtacagtccg cccgtaaata tttaacctac cgtctccctc
      181 gccctaacaa agcggcgata accccgccaa ttcccacgaa cggcgagcaa atcatcgccg
      241 caaagcccat gaagagcaag gctttggcag agtctttttg gtattgctca aatgccaccc
      301 ggtcaccagc gaaaagttct tctggcacca ctccatctcc agaagatttc ttgccgagca
      361 cggtcaccaa atcaccgctg aaaaagcctg tataacgaag ggaaccttct cctaacttct
      421 gtcctttgta atcggcagtg aaagcgctgg attttgtcac cagctgctca tgaaggtttg
      481 agccgcttaa cgtcgcatta tttgccgaaa ggatgttaac cggcgttccg ctcatatcca
      541 gggttaagtc tggaacgatt gtctccacat gttcccaaga accagaaggc gcttggggtg
      601 tgccatcggt attcttgttc tcactcacat cccattgttc tgcttcataa gcaataaact
      661 tcggcaattg cgggttcggt gcagacccat tcaaataacc cgtgatcaaa atatccgtgc
      721 cgggggcagc agcactcaca taggcggcat ccatttgcgg catcttctca atatttgcgg
      781 ctttgatcgc ctgttgcggt gccagcacaa atgccatcaa tgcgccacac agtgccataa
      841 agatcgcagc accaatggca gccaccgccc caccaattcg attggtaact gtcctaccta
      901 tattgccggt aaaattttgc atactcattc tcctcatttg gtttgatgcg gcattataaa
      961 atttattccg catcggcatc atccactgga agtactaatc aggctccatt tcaataccat
     1021 gttcgaagaa gttttcagac acaatcgctc taatttccgc tgtataattc gtccatggca
     1081 gattggatcg gacaaacgat tggcaaagtc cgtgtagaaa aatatctggc acgcggggga
     1141 atggcagagg tctacttggg cacgcacctc actctcgacc gccccgtagc ggtcaaagtg
     1201 atgcatagct tcgtggaaag tgaacccgaa ctaatcaacc gcttccaacg tgaagccaaa
     1261 gccgtcgcgg cactgcgtca tcccaatatc gttcaagtct acgacttcga cacccacgat
     1321 gggcatccct atcttgtgat ggaatatctc aaaggtccat cgctggcaag tctcttgcgt
     1381 gaacttcaca agaacggtgc acgtttatcc ctctctcaaa tcgcacaatt actcaagtcg
     1441 ctgacggcgg gcattgacta tgctcattcg caaggcatcg tccaccgcga tatcaaaccc
     1501 gccaacatta tcctcaacaa caaaagcggc gactacacca tcgaccaact tccgcgtgat
     1561 accgaatgca tcatcacaga ctttggtttg gtgaggctca caaactccac cactcaaacc
     1621 gcgtcagggg ttgtcagtgg cacgccagcc tatatgtcac cagagcaggc acaaggctcc
     1681 gtggtagata gtcgctccga cacctattcc cttggcatcg ttttatatga aatgttggcg
     1741 ggacgtttgc ctttcgatgg cgactctacg ttgggcgtaa ttctcaagca catctctgaa
     1801 ccgccgccac ccatcgaaga catctcacct gaaattcaag ccgttgtcaa caaagcactt
     1861 gaaaaacttc ctgaaaaccg atatcaaacc gcgcgtgacc ttttgatgga tttttacaac
     1921 gcagtgggca tgcatgcaga agccgagact ttatattccc tgcgcctccg caccccgccg
     1981 ccaactgccg ctcttagcaa taagcctgct cctaaaccaa accgcttcct ttggatcggt
     2041 gccggaattc tggcatgttt atgtttaggg gttatcgcca caggtgcagt cggattttcc
     2101 atttattcat tgttccccaa agcgaccact acaccacaaa ttctcaccga tgccgccacg
     2161 ccagaccaca tgcctcatgg caacacagca gaacccggct ccacgcctca gcccatctcc
     2221 tttggtgaat tttttggtgt attacgcttc caaggcaaca tggaccaagt cacagtcacc
     2281 gcctccctgc cagacccggc agaaggcacg caatatgaag cctggctgat cgacgatggc
     2341 ggcgaattac tcatcagcct tggaatcttg gaaaagaata attccggtca atacaatctg
     2401 acgtttactg accctgaaag ccgcaatcta ttagacggtt tcaatcgcat ggaaatcacg
     2461 ctcgaaacca atccagatgc cagccccaac ccatccgaca cgatcttgta ttcctctggc
     2521 atccccgccg gtgcgttgac tcacgttcgg catttgttag tcagtttccc cagcaacccc
     2581 aaccagaccc ccatgacggg cgggctgata ggaaccagca gtctgatcaa tgaggctggg
     2641 acagcactgg agaaagcctt tgaaaaaggc gatacgactg caattcgctt gcaagccgag
     2701 acgttgatca accttgttgt gggcaagcaa agcaacgaat acaaagactg ggacaagagc
     2761 ggaaagatca gtgaccctag cgatggcttt gggcttttgt tgaacggtgg tcagaccggt
     2821 tatattgaag ggacgattac ccatgccgaa ctttctgctt cggcgggcga tgccactgca
     2881 aatatcaaac ttcacagtga gcatgtcgtg atcagcgcga aaaatgttga gggatgggca
     2941 acccagcttc gagatgtcgc gaagcggatt ctcgagtcag accaggtttc tgagtccgat
     3001 atccgcttgg cagtttcact tgcagaacag atttatcagg gtgtggatat tgatggcaac
     3061 gaaagtgtgg acccggttga aggggaaggc ggggcattga cggcatatca acatgcagaa
     3121 tatatggcag atatgcaggt ccttgaaggc gcaaatcttt tcccaccacc agggaacccg
     3181 tagagtaaac gtcgtgcctt tcgcacgacg tttttatttc aatagaaaac ccatgccaga
     3241 gtaggttgaa tcttggtggc aatcgttata taaagaataa atacttactc gaggagagtt
     3301 tcatgactat agtaactgct gagacgttcg tacaagcccc tttgaaattt gcctaccgag
     3361 cctttaccaa tgccacaacc attcgcgaat ggttgtgtga tg
//
LOCUS       DAOQJE010000322         3401 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_17935_length_3401_cov_4.385236, whole genome shotgun sequence.
ACCESSION   DAOQJE010000322 DAOQJE010000000
VERSION     DAOQJE010000322.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3401)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3401)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3401
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17935_length_3401_cov_4.385236"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            325..1041
                     /locus_tag="PLT08_09325"
     CDS             325..1041
                     /locus_tag="PLT08_09325"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HNE04707.1"
                     /translation="MKLSIIVPAYNEAKTIHLILNKVIEASLLNDTKKEIIIINDASK
                     DSTETVVQEYIKAHSNEDIRLFSLQYNQGKGAAIHKGIELASGDFIIIQDADLEYDPN
                     EYNILLKPILDNHADVVFGSRFVGGKPHRILFFWHSIGNKFVLTGLSNIFNNLNLTDM
                     ETCYKLFRAEHLKSIKLTEKRFGFEPEVTAKISRIPNIRIYEVGISYYGRTYEEGKKI
                     GWRDGFRAIYCILKYGLFRL"
     gene            1095..1631
                     /locus_tag="PLT08_09330"
     CDS             1095..1631
                     /locus_tag="PLT08_09330"
                     /inference="COORDINATES: protein motif:HMM:NF022167.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside nucleotidyltransferase
                     ANT(2'')-Ia"
                     /protein_id="HNE04708.1"
                     /translation="MHTSHIPLIHQLLASAEKQNLPLWLESGWAMDARLHKITREHDD
                     IDLAFPMEQQDQFLMLLQAFGSEKLEKTDYGFLVYIQGVLLDCEPCIQHGDTYELENV
                     PLNSCPWEKQGSIDGIAVRCISWEAILWEYFYYLDEVPQDSWRPKDFDSFDIVQKSLG
                     EAKVKQLHIAFESAKNNL"
     gene            1707..2318
                     /locus_tag="PLT08_09335"
     CDS             1707..2318
                     /locus_tag="PLT08_09335"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acyl homoserine lactonase family protein"
                     /protein_id="HNE04709.1"
                     /translation="MKKEITITSIRVADLLAEGERMPVYVHVIDHPNGRVLVDTGFTE
                     LHPAVADLDPRIFPLNEQNVDLASIDIVVNTHLHFDHCGGNHLFPGKPIYVQQAELDD
                     ALTKDDYTIREWVDAPGVKYMPVDGEFELLPGLRLVPAPGHTRGMQMVVVETGERPII
                     VGGDVAVWFGELDEPKTEGQLKVHTLNPQLVWLTHEHEPWRPK"
     gene            2378..>3401
                     /locus_tag="PLT08_09340"
     CDS             2378..>3401
                     /locus_tag="PLT08_09340"
                     /inference="COORDINATES: protein motif:HMM:NF013702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HNE04710.1"
                     /translation="MAYSIKYPRKVFIRSTFRFTIRSLFPLLAQTKITGSKNFPQNGP
                     LILVGNHTGAMEVVLMGLYSPKPVEFMGAMEMPWNGWMGKMIDLYGLIPVHRGYTSNT
                     TMKMGIDVLRQGGILGIFPEGGFWEPGKQKAQSGVAWLSYAAQAPILPIGFGDTRGKM
                     AEIFQLKRPAFEMNIGDVIPPVQLEKSAHKKEELQQAADRIMDAVWALVPEDERKRKE
                     SQPENELFTFDISIFDQHGTPIPIPADLQLTDGSWISRFAHRPNLIDSIRDYIFIPVQ
                     VLKELHRKPPAAEIYQAAHSMLEYVERDNPQYFNYRYGYKDGEIFQQSFRQLRDLMKW
                     AMENQYQ"
BASE COUNT         1079 a          679 c          741 g          902 t
ORIGIN      
        1 gctggtggtg atcggcgata gcgccacgat tacggcgcat ccgttctacg agaagctggt
       61 gaagtatttt gaagggattg gggcgtatca ctcggtgtgg gaggaataaa aaaaggcggc
      121 gttatttgct tatatttttt gttactcttt tattcgaagt agcttataat ttatttatcc
      181 acacaaaata ggttaaccaa aatcactata cataaaaaaa accgtagggt ggtaaaaaag
      241 ctaaaaggct taattatgca aagttaaaca tctcgctgaa aattaaagaa agagtagatt
      301 aaatacatcc gaggaaatta attcatgaag ttatccatta ttgtcccagc ctataatgaa
      361 gcaaaaacca ttcatttgat tcttaataaa gtaattgaag catctttatt aaatgacaca
      421 aaaaaagaaa tcatcattat caacgacgcg tcaaaggata gcacagagac agtagtacaa
      481 gaatatataa aagcccattc taatgaagac ataagattgt ttagccttca atacaatcaa
      541 ggcaagggag ccgctatcca taagggtatt gaattagcgt caggcgattt tataataatt
      601 caagatgcag atttagaata cgacccaaac gaatataata ttttactcaa accgatttta
      661 gataaccatg ctgatgtagt gtttggttca cgttttgtag gtggaaaacc tcacagaata
      721 ctttttttct ggcactcaat aggtaataaa tttgtgctaa caggtttgtc aaatatcttt
      781 aataatttaa atcttacaga tatggagaca tgttacaaac tatttcgcgc agaacactta
      841 aagagcataa aacttacaga aaaacgtttt ggctttgaac cggaagtaac tgcaaaaata
      901 tcgagaattc caaacatacg aatttatgaa gttggtattt cttactatgg aagaacctat
      961 gaagaaggaa aaaagatagg ttggagagat gggtttcggg caatttactg catattaaaa
     1021 tatgggcttt ttaggctata aaaattccaa ccatttataa caaagcatct caaagagtgt
     1081 tgggataata aattttgcac acatcccata ttccattaat tcatcaacta ctagcaagcg
     1141 ctgaaaaaca aaaccttcca ctctggcttg agagcggttg ggcaatggac gcccggcttc
     1201 ataaaataac acgcgaacat gatgatatcg acctagcgtt tcctatggaa cagcaagatc
     1261 aatttctaat gttattacag gcatttggaa gtgagaaatt ggaaaagact gattacggat
     1321 ttctcgttta catccaaggt gtgctattag attgcgagcc ttgtatccaa catggagaca
     1381 cttacgaact tgagaacgta cctttaaact cttgcccatg ggaaaaacaa ggaagtattg
     1441 atggcattgc ggtccgctgc ataagttggg aggcaatatt atgggaatac ttctactatt
     1501 tagacgaagt ccctcaagac tcatggcgac caaaagactt cgatagtttt gacatcgtgc
     1561 aaaagtcact tggtgaagca aaagtcaaac agcttcatat agcatttgag tctgccaaga
     1621 ataatttata agcataaaga ttttgtggag tttctcgcct tgacgaaata gaaaatacgt
     1681 tctattatca acgaaggaga aatttcatga aaaaagaaat caccatcacg tcaattcggg
     1741 ttgcagactt gcttgcagaa ggtgaacgga tgcccgtcta tgtacatgtc attgaccatc
     1801 caaatggacg tgtgttagtg gatacagggt tcaccgagtt gcacccagcg gtagctgacc
     1861 ttgacccgcg catctttccc ttgaacgagc agaatgttga ccttgccagc attgatatcg
     1921 tcgtcaatac acatttgcac ttcgatcatt gcggtggcaa tcacctcttc cccggcaaac
     1981 cgatctacgt tcagcaagca gagttggatg atgctctcac caaagacgac tacaccattc
     2041 gcgagtgggt tgatgcgccc ggggtgaaat atatgccggt cgatggtgag tttgaactgc
     2101 ttcccgggtt gaggcttgtc cctgcaccgg gtcatacccg tgggatgcaa atggtcgtcg
     2161 ttgagacagg tgaacgccca atcatcgtcg gcggtgacgt ggcagtttgg ttcggcgagc
     2221 ttgatgagcc gaaaactgaa ggtcagctaa aggtgcatac acttaacccg cagttggttt
     2281 ggctcacaca cgagcatgag ccgtggcgac cgaagtaatt aatcaaactt gatatattca
     2341 aagaactcta tcaagaggta aaatagccgt cattaatatg gcgtattcca tcaaatatcc
     2401 ccgcaaggta tttattcgaa gtacgttccg ttttaccatt cgctcgcttt tccccttatt
     2461 agcacaaaca aaaattacag ggtcaaaaaa cttcccacaa aacggacctc ttattctggt
     2521 tggaaatcac actggcgcaa tggaagtcgt tctcatgggg ttgtattctc ccaagcccgt
     2581 agagttcatg ggtgcaatgg aaatgccctg gaatggttgg atgggaaaaa tgatcgacct
     2641 atatggattg atccctgtcc accgtgggta taccagcaac acaactatga aaatgggaat
     2701 tgatgttttg cgtcagggtg gaatattggg aatttttccc gagggcggtt tttgggagcc
     2761 cggtaagcaa aaggcacaat caggggtagc atggttaagt tatgcggctc aagcgccaat
     2821 tctccccatt ggctttggcg atacgcgcgg caaaatggca gaaatatttc aactaaaacg
     2881 ccctgccttt gagatgaata ttggagatgt aattccgcct gtgcaattgg aaaagtctgc
     2941 acacaaaaaa gaggaattgc aacaagccgc agaccgtatt atggatgcag tttgggcgtt
     3001 ggtaccagaa gacgagcgca agcgcaaaga aagtcaacct gaaaacgaat tatttacttt
     3061 tgatatatcc atctttgacc aacatggaac gcccataccg attccagcag atttacagct
     3121 aacagacggc tcatggatca gtcgcttcgc acaccgcccg aacttgatcg attcgatccg
     3181 agactatatc ttcatcccgg tacaggttct aaaagaacta caccgaaagc ctcctgcagc
     3241 agaaatttat caggcagcac attctatgct tgaatatgta gagcgcgata accctcaata
     3301 ctttaattat cgctatgggt acaaagacgg cgaaatcttt caacagagtt tccgtcaact
     3361 acgcgacctg atgaaatggg caatggagaa tcaatatcag g
//
LOCUS       DAOQJE010000323         3397 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_17969_length_3397_cov_4.213645, whole genome shotgun sequence.
ACCESSION   DAOQJE010000323 DAOQJE010000000
VERSION     DAOQJE010000323.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3397)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3397)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3397
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17969_length_3397_cov_4.213645"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..887
                     /locus_tag="PLT08_09345"
     CDS             <1..887
                     /locus_tag="PLT08_09345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE04711.1"
                     /translation="KDATPYYLRPDLREDYMRMTIDSFSINKSAALAADRWLGLGSTA
                     QEAYLAIQNNPGYLPSALVQEFGQAVTNEINARGGVPADPSTGMSPTMRTALIGIGVI
                     LLLGVVAAGGMYLFRLFGKRGSGEVTVVMQAAEISRAAAKTNFEDMGLAPPITQTMTT
                     YVLGDDLYDESFSIDTAAGEFLGEYGVGVSEIIGVGEPKKVTALEIWLFDKNDIKTAT
                     KVLMSRHAFADPGIRSRLEPKGELVLVEPQAQVLLETATLQLLATIVDIEYGKGPLPD
                     SSYFERVTLELAIWPRQG"
     gene            complement(932..1396)
                     /gene="greA"
                     /locus_tag="PLT08_09350"
     CDS             complement(932..1396)
                     /gene="greA"
                     /locus_tag="PLT08_09350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HNE04712.1"
                     /translation="MTQPNYLTPEGEAKLKAELEELKGPRREELAQRLRSAIQMGDLS
                     ENADYHKAKEDQGFLEGRIQEIEAILRNSVLIEKTASSDVVSIGSHVTIQEEDFDPET
                     YHIVGPTEADPRKGKISHESPIGVALMNKKVGQIAEAQAPGGKIKFKILKIE"
     gene            complement(1485..2636)
                     /locus_tag="PLT08_09355"
     CDS             complement(1485..2636)
                     /locus_tag="PLT08_09355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016793 - triphosphoric monoester
                     hydrolase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyguanosinetriphosphate triphosphohydrolase"
                     /protein_id="HNE04713.1"
                     /translation="MFFTRQQLEENEDRSLAPYGMRSKDTKGRAYLDAEPEYRTTFQR
                     DRDRILHTTAFRRLEYKTQVFINFEGDHFRTRLTHTLEVAQIGRTLARALGGNEDLVE
                     AICLAHDLGHSPFGHSGETALARLMKDYGGFDHNKQSLRIVTELEQRYPEFPGLNLSW
                     EVREGMVKHESEYDISDARDFSPDLRGNLETQIANVADELAYTTHDLDDGLRSGMINP
                     QILEGVALWEILRETYNWHGSILSDMERHRMIRHLVGILVTDMVKGTDARLKESKVNS
                     ALDIQKLKHNVVGYTEEMQRRNRELKDLLYKKLYRHYRVVRMQVKAERIISDLFNAFR
                     AEPATLPDHVQFFIEKRGLERTICDYIAGMTDRYAIEEHQKMFNPNEKP"
     gene            complement(2703..2786)
                     /locus_tag="PLT08_09360"
     tRNA            complement(2703..2786)
                     /locus_tag="PLT08_09360"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2750..2752),aa:Leu,seq:taa)
     gene            3119..>3397
                     /locus_tag="PLT08_09365"
     CDS             3119..>3397
                     /locus_tag="PLT08_09365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE04714.1"
                     /translation="MKTSGPLLLTLFLLACSLTTPPSLPKDMPAQVSNKTHLATATPD
                     PNSNSHTMPVTCTVSAQSLHLRECAGLHCTVRAWLSTGDTLEVLDADQD"
BASE COUNT          808 a          810 c          846 g          933 t
ORIGIN      
        1 ttaaggatgc aaccccatat tatttgcgcc ctgatttgcg cgaagattac atgcgcatga
       61 cgattgactc ttttagcatt aacaaaagcg cagcattggc agccgatcgt tggttggggt
      121 tgggcagcac agcgcaagaa gcctatttgg cgattcaaaa taaccccggt tatttacctt
      181 ctgctttggt gcaagagttt ggtcaggcag tgacgaatga gatcaatgca cgcggtggcg
      241 tacccgctga cccctctacg ggtatgtctc ctaccatgcg cactgcgttg atcggcattg
      301 gtgtgatttt gttactgggc gtcgttgccg ccgggggcat gtatctcttc cgcttgtttg
      361 gaaaacgagg ctctggcgaa gtgaccgtag tgatgcaagc cgctgagatt agccgagcag
      421 ccgccaagac aaattttgaa gatatgggtc tggctccacc cattacgcag accatgacaa
      481 cttatgtctt gggtgatgac ctttacgatg agtctttcag cattgacaca gccgcaggtg
      541 agttccttgg tgaatacggc gtgggcgttt ctgaaattat tggcgtgggt gagccgaaga
      601 aggttactgc gttagaaatt tggctttttg ataagaacga catcaaaaca gcgaccaaag
      661 ttctgatgtc tcgccatgct tttgcagacc caggaattcg ctcgcgcctc gaacctaagg
      721 gtgagttggt tcttgtggaa cctcaagctc aggtgttgct cgaaacggca accctgcaac
      781 tcctcgcaac cattgtggat attgaatatg gcaagggacc tttgcctgat agcagctact
      841 ttgagcgtgt gacacttgaa ttagcaattt ggccccgcca aggataaata taaacaaaaa
      901 aacgctccac atacgtggag cgtttttttg tttattcaat ttttagaatt ttgaacttga
      961 ttttgccgcc gggcgcttgt gcttcagcga tttgcccgac ctttttgttc atcagcgcaa
     1021 ccccaattgg ggattcgtga gagattttgc ctttgcgtgg atctgcttcg gtgggaccga
     1081 cgatatgata cgtctctggg tcgaagtctt cttcttgaat ggtcacatga gagccaatgg
     1141 agacaacatc tgaggaggct gtcttttcaa ttagtactga gttgcgcaaa atggcttcga
     1201 tctcttgaat gcgaccttca aggaagcctt gatcttcctt tgccttatgg taatcggcat
     1261 tttcggagag gtctcccatt tggatggcag aacgcaggcg ctgtgccaat tcctcgcggc
     1321 gaggtccctt cagttcttcg agctcagcct ttagtttggc ttcaccctcg ggggtcaggt
     1381 aattgggttg tgtcatggtt tctcctttcc tgatgtgcgt tggataagac gataaggggc
     1441 ttgatacgaa ctctatctct atgccccctt agaaacgaaa gaaattaagg tttctcgttg
     1501 gggttgaaca ttttttgatg ttcttcgatg gcgtaacgat ctgtcatgcc ggcaatgtaa
     1561 tcacaaatgg tgcgctccag tccgcgtttt tcgatgaaga attgcacatg gtctggcaat
     1621 gtggcaggct cggcgcgaaa agcattgaaa aggtcagata tgatccgctc tgctttgact
     1681 tgcattcgta caacgcgata atggcgatac aactttttat agagcaaatc ttttagctcg
     1741 cgattacgtc tttgcatttc ttcagtatag ccaactacgt tatgtttcag cttttggata
     1801 tcaagcgctg aattgacttt gctttctttc aagcgggcat ctgtgccttt gaccatatct
     1861 gtgaccagaa ttcctaccaa gtgacgtatc atgcggtgac gttccatatc actgagaatg
     1921 cttccgtgcc agttatacgt ctctcgcaag atttcccaaa gcgccacgcc ttccaaaatt
     1981 tgtgggttga tcatcccaga gcgtagacca tcatcaaggt cgtgggtcgt gtaagcgagt
     2041 tcatctgcca cattagcgat ttgggtttcg aggtttccac gtaaatctgg gctgaagtca
     2101 cgcgcatcag aaatatcata ttctgactcg tgcttcacca taccttcacg cacttcccaa
     2161 ctgagattca aaccggggaa ttctgggtaa cgttgttcca gttcggtcac aatgcgtaac
     2221 gattgcttat tgtggtcaaa accgccgtag tctttcatca atcgcgccag agctgtctcg
     2281 ccagagtgac cgaagggaga atgtccaagg tcgtgtgcca aacaaatggc ttcaaccaag
     2341 tcttcgtttc caccaagcgc acgcgcaagc gtccgcccaa tttgggcaac ttcaagtgta
     2401 tgggtcaggc gggtgcggaa gtgatcccct tcgaaattga taaagacctg agtcttgtat
     2461 tcaaggcggc gaaatgccgt tgtatgcaga atacgatctc gatcgcgctg gaatgtcgta
     2521 cgatattccg gctctgcgtc gagataagca cgtcccttgg tatctttact acgcatccca
     2581 tagggagcaa ggctgcgatc ttcgttttct tccaattgtt ggcgtgtaaa gaacatggtc
     2641 tgtccttaga tgcaaatgtc attgcaacaa tctgtgttga gtgttgcaat gacagtggag
     2701 agtggggcga gagggggtcg aaccctcacg ttgtttccaa cgacagattt taagtctgtt
     2761 gcgtctgccg attccgccat cgccccggca gaagatattt tactataact tgctttttat
     2821 gccacaaaaa ataaatcgcc agtgaaggcg atttatttgg cggtcccgac gggcgaaaat
     2881 tccaaagttt ttgcgatgga atcgaactac ttccgcccgg catacaaaag tcagaagctc
     2941 tgacaatttt ggactatcga agaaaacgtg atctcaaata gccattaacg cagctcaatg
     3001 caaagagcgg cgctcgcaca ttcgtgtgtg ggcgccgttt tttttatttc acaacaaagg
     3061 agatcacgcg tatgaaagac aatcagtttg cacgatcgac accatcaggg aggacaccat
     3121 gaaaacatcg ggaccgctgt tactaactct atttttattg gcttgttcac tcaccacgcc
     3181 accatcgctg ccaaaggaca tgccggctca ggtgtccaac aaaacgcatt tagcgacagc
     3241 aacaccagac cccaattcca actcccatac aatgcccgtt acctgcaccg tatcggcaca
     3301 gtccctgcat ctgcgggagt gcgccggctt gcattgcacc gtgcgtgcct ggttatcgac
     3361 gggtgacaca ctggaagttc tggatgcgga tcaggac
//
LOCUS       DAOQJE010000324         3391 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_18008_length_3391_cov_7.014089, whole genome shotgun sequence.
ACCESSION   DAOQJE010000324 DAOQJE010000000
VERSION     DAOQJE010000324.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3391)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3391)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3391
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18008_length_3391_cov_7.014089"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..959)
                     /locus_tag="PLT08_09370"
     CDS             complement(<1..959)
                     /locus_tag="PLT08_09370"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HNE04715.1"
                     /translation="MKILTVLTYYRPHTSGLTIYAERLAHAFVKRGHQVTVMTTQYDP
                     SLPREEVMDGVKVIRVPVLARVSKGVLAPTFGLVATKLAWEHDVVQMHLPQFDAPGVA
                     FRARLFGKPAVLTYHCDIQLPQGVFNRLVNAVVNFQNHMAGILSNHIVTYTQDYADHS
                     PYLSHYASKLTPILPPVELPDPLPGAVSAFASTHLTQERRPVIGMAARFASEKGVEVL
                     LDAMPAILKKYPKAQVLFAGTYQYVMGEQAYSDRLMPRIHEYEVNGNWKFLGNLDPVQ
                     MAAYYPNLDVITVPSLNSTEAFGLVQIEAMMNGVPSVPSALPGV"
     gene            complement(956..1786)
                     /locus_tag="PLT08_09375"
     CDS             complement(956..1786)
                     /locus_tag="PLT08_09375"
                     /inference="COORDINATES: protein motif:HMM:NF013117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HNE04716.1"
                     /translation="MPHQLLSLVDVAMATLPNVLISLAIFAVSYYAGVWLGRLLKGVL
                     TRKNAEPGVSHLLSQILKWTIISLGFITALQRFFDVTAFLTGLGIIGFTIGFALQNIM
                     QNFVSGIILLIQQPFKVSDEVSLMGNEGFVQKIGLRTTEIRLRNGLLVFLPNADVLSQ
                     PIINFSRANGFRVELPVSIPYEADTEKARSIILDQVRTVPGCLETPVAQVFFHTFNAS
                     SVDLRVQFWVDNRTTDHHAAKDMVLVRIKQAFEENGIDVPYPVQVMKNPTKVTKRKTK
                     "
     gene            complement(1897..2925)
                     /locus_tag="PLT08_09380"
     CDS             complement(1897..2925)
                     /locus_tag="PLT08_09380"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: protein motif:HMM:NF027681.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HNE04717.1"
                     /translation="MKNKQYLVTGGAGFIGSNYVHRLLKRGEKVTIYDNFSRGGAPRN
                     LEWLKAEFGEKAFEVVVGDVRDAARIAEAAKTSDVIVHLAGQVAVTTSVTHPRDDFEA
                     NALGTFNVVEAARASGRQPIVIYASTNKVYGGMEEVELFEEPTRWRYKDLIRGCPETQ
                     PLDFHSPYGCSKGTGDQYVRDYARIYDLPTVVFRQSCIYGPRQFGIEDQGWVAWFIIA
                     AVMGRNITIYGDGKQVRDILHVHDLIQAYDLAVEKIDVARGQVYNLGGGPSNVMSIWS
                     EFGPRLEALLGKKIEVGRGDWRPGDQRVFYADVSKAKNELGWEPKISVEQGVNMLFEW
                     VQANKNLF"
     gene            complement(2915..>3391)
                     /locus_tag="PLT08_09385"
     CDS             complement(2915..>3391)
                     /locus_tag="PLT08_09385"
                     /inference="COORDINATES: protein motif:HMM:NF015651.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase
                     domain-containing protein"
                     /protein_id="HNE04718.1"
                     /translation="FHKLSGRIPALQKIGGSFLESFFQGLAVLTDGWLFLRFLFWMIL
                     NWAIALFAYYLMVLAFFPEAKPIWAIFGLGMAAFGGAVPSAPGAVGTFEGAVVFALTR
                     FTSDESTAFAAALTMRIYNYLNSTVVGMLGLSSEGQTLSGVYKQLINFRNRKTDEE"
BASE COUNT          970 a          835 c          778 g          808 t
ORIGIN      
        1 acccctggca aagcagaggg cacactgggc acaccgttca tcatggcttc gatctggact
       61 agaccaaacg cttcagtcga attgagagat ggtacggtaa taacatccaa gttcgggtag
      121 taagctgcca tctgaacagg gtcgagattg ccaaggaatt tccaattccc atttacttca
      181 tattcatgaa tgcgtggcat caggcggtct gaataagctt gctcacccat cacatattga
      241 tatgtccctg caaataacac ttgagcctta ggatatttct tgaggatggc aggcatcgca
      301 tcaagcaaga cttctacgcc tttttcagaa gcaaaacgcg ctgccatacc aattacgggt
      361 ctacgctcct gcgtaagatg agtggatgca aaagcggaaa cagccccagg gagtggatca
      421 ggcaactcaa cgggtggcag gatgggggtc agttttgagg cgtaatgtga gaggtacgga
      481 gaatgatccg catagtcctg tgtgtacgtc acgatatgat tcgaaagaat ccccgccata
      541 tggttttgaa aatttactac agcattgacc aaacgattga atacgccctg cggaagttga
      601 atatcgcaat ggtatgtaag gacagcaggc ttgccaaaca aacgcgcacg gaaggcaacg
      661 ccgggcgcat caaactgcgg aaggtgcatt tgaacaacat catgttccca tgccagtttg
      721 gtagcgacca acccaaaagt tggggcaagc actcctttgc tgacacgcgc aagcacagga
      781 acacgaatca cttttacacc atccatcact tcttcacgtg gcagggatgg gtcgtattga
      841 gtggtcatta cagtgacttg gtgcccgcgt ttgacaaagg catgtgcaag ccgttctgca
      901 taaatagtca gcccagaggt atgtgggcga taataggtta gtacggtcag tatcttcatt
      961 ttgtctttcg tttcgtcact ttggttggat ttttcataac ttgaactggg tacggaacgt
     1021 cgatcccgtt ttcttcaaat gcttgtttga ttctgaccaa caccatatct ttcgctgcat
     1081 gatggtcggt agtgcgatta tccacccaga actgtacgcg taaatcgacc gatgaggcgt
     1141 taaaggtatg aaaaaatact tgggcaacgg gtgtttccaa acaaccgggc acggttctaa
     1201 cttggtcaag aataatggaa cgcgcctttt cagtatcggc ttcataggga atgcttaccg
     1261 gcaattccac acgaaagcca tttgcccggc taaagttgat gatcggttga gaaagcacat
     1321 cggcattggg tagaaaaacc agcaacccat tgcgtaagcg aatctcagtg gtgcgcaaac
     1381 caatcttctg aacaaagcct tcgttcccca tcaaacttac ttcgtcactt actttaaatg
     1441 gctgttggat caagagaata atgccagaga caaaattctg catgatgttt tgcaaggcaa
     1501 agccaatggt aaagccaatg atgcctagac cggttaagaa ggcggtaaca tcaaaaaacc
     1561 tttgcaaagc agtgatgaaa ccaagggaga tgatcgtcca cttaaggatc tgtgaaagca
     1621 aatgcgaaac gccaggctcg gcattcttcc gggtgagcac gccctttagt aaacgaccca
     1681 gccaaacgcc ggcataataa ctcacagcaa aaattgccag cgaaattaaa acattgggca
     1741 gtgttgccat ggcaacatcg actaaagata aaagttggtg aggcatataa ggatgggttt
     1801 cttttcttga atggaaaatc tagcctaggt attttaccta ataaagtacg gctgtaaaaa
     1861 ttcgggttga acttttacag ccgtttgata tttactttag aagagattct tatttgcttg
     1921 cacccattca aacagcatgt tcaccccttg ttccacgctg atcttgggct cccaaccaag
     1981 ttcgttcttg gctttagaaa catcggcata aaagacccgt tggtcgccgg ggcgccaatc
     2041 gccgcgccca acttcgatct tcttcccaag caacgcttcc aaacgcggac cgaactctga
     2101 ccagatcgac atcacatttg aaggtccgcc gccgaggtta taaacttgcc cacgagccac
     2161 gtcgatcttt tcaacggcaa ggtcatacgc ttggataagg tcatgtacat gcaaaatatc
     2221 gcgcacttgt tttccatcgc cataaatcgt gatgttccgt cccataaccg cggcaataat
     2281 aaaccaagcc acccaaccct gatcttcaat gccaaattgg cgcggaccat aaatacaaga
     2341 ctggcggaaa actaccgttg gcaaatcata gatacgagca taatcgcgca cgtattggtc
     2401 gccggtgcct ttagagcacc cataaggcga gtgaaaatct aaaggctgag tttcagggca
     2461 accacggatc aaatctttat aacgccaacg agtgggttct tcaaataact ctacttcttc
     2521 catgccgcca tacactttat tcgtggaagc atagatcaca ataggctgac gtcccgacgc
     2581 gcgtgcggct tctacaacat tgaaggtccc taaggcattg gcttcaaaat catctctagg
     2641 atgggtgact gaagtggtca ctgcaacttg acctgccaaa tgcacgatca catctgacgt
     2701 tttagctgcc tcagcgattc tggcagcgtc tcgcacatcc ccaaccacaa cctcgaaagc
     2761 cttctcacca aactcagctt tcaaccattc caaattacgc ggagccccgc cacgcgagaa
     2821 gttatcgtaa atggtcacct tttcaccgcg ttttagcaag cgatgaacat aatttgaacc
     2881 gataaaaccg gcaccacctg taacgagata ctgtttattc ttcatctgtt tttctgttcc
     2941 taaaattaat tagttgtttg taaacacctg aaagggtttg cccttcgctc gataacccga
     3001 gcattcccac aacagtacta ttgagatagt tataaatacg catggtaagt gctgctgcaa
     3061 acgcagtaga ttcatcgctg gtgaagcggg ttagggcgaa gacaacagct ccctcgaagg
     3121 tgccaaccgc tcccggcgca gaaggaaccg cgccgccgaa ggcggccatc ccaagcccga
     3181 agatcgccca gatcggtttg gcttcgggga aaaaggcaag aaccatcagg taatatgcaa
     3241 acaaagcaat tgcccaattt agaatcatcc aaaataaaaa gcgcaggaag agccagccgt
     3301 cagtaagcac agcaagccct tgaaagaaag actcgagaaa actaccgcca attttttgca
     3361 aagctgggat gcgcccgctc agtttgtgga a
//
LOCUS       DAOQJE010000325         3390 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_18026_length_3390_cov_3.671064, whole genome shotgun sequence.
ACCESSION   DAOQJE010000325 DAOQJE010000000
VERSION     DAOQJE010000325.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3390)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3390)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3390
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18026_length_3390_cov_3.671064"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            51..605
                     /locus_tag="PLT08_09390"
     CDS             51..605
                     /locus_tag="PLT08_09390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE04719.1"
                     /translation="MKTFFRKRLAQILPLVLSVYCAAVQIYTDNKSLHLFCDRDMFIL
                     IDHYGEKRVYFDNGKEELFVGYLTEYSLPGMRTLFVEISKTKRCMIGKVDLPGDFIDV
                     RVNGFGLHDTQKIPLDDGYFIMPIIGVDVYGITAFDLTVNDNGNRHDVSKKFDPPFSP
                     YYFSVADIAEDYEWSRAKSRSALP"
     gene            complement(1041..2006)
                     /locus_tag="PLT08_09395"
     CDS             complement(1041..2006)
                     /locus_tag="PLT08_09395"
                     /inference="COORDINATES: protein motif:HMM:NF012798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HNE04720.1"
                     /translation="MENDLLEHFEHYLNDNNSPTSVKGYLSDIRAFIGWAKGHKGIEL
                     NRSLLKGQPHLLNKAVIDAYLKHLHAEKYALNTIARHGAAMRVFGTFLLSSGIVPVNP
                     AEKIKNVKPDPSDPRGLDDDQRARLEYVFRTPWERTANKTSRRREKTLAPKMLIRDRA
                     MMLTMMYAGPRISELIGLDVSDVTIKERSGNIIIRDGKGGKPRVVDIPRQVREAIADW
                     LDVRREFPAADDALFIGLKRGHSRITQRTVQLMLEEASKRSGVEVTPHILRHTYAYML
                     MKNGTHPKTAAALMGHSLEMALRYGSSKEEDKRKAVDGLDDLSTF"
     gene            complement(2022..2276)
                     /locus_tag="PLT08_09400"
     CDS             complement(2022..2276)
                     /locus_tag="PLT08_09400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE04721.1"
                     /translation="MNNKKQIDKDFEYWIAWAERTKALPNLVAELRTRADETGRKEAV
                     ALLRAIHTRRRSEIRGGVIAAVIVIVVIVGTVIAIIAAFV"
     gene            complement(2269..2454)
                     /locus_tag="PLT08_09405"
     CDS             complement(2269..2454)
                     /locus_tag="PLT08_09405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE04722.1"
                     /translation="MKTMKLFIGITELIMALVALIVMFTSVHPIAKAGILLVVLFGGK
                     GIFYVYKGLSRKGEEYE"
     gene            complement(2495..2836)
                     /locus_tag="PLT08_09410"
     CDS             complement(2495..2836)
                     /locus_tag="PLT08_09410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE04723.1"
                     /translation="MHDDFDLLMTVHYPDDLAEAFGYNSDARYVGFYWDHAGDELTWL
                     DGRRSLCGAECQYFLKFASLHQINKHRIGDSDTLPTNYLLVDRKSNNLYVGDKRLVEE
                     FLKLQWSPIQS"
     gene            complement(2843..3160)
                     /locus_tag="PLT08_09415"
     CDS             complement(2843..3160)
                     /locus_tag="PLT08_09415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE04724.1"
                     /translation="MMTGPNYVLLIILALIAVLGDIGAIEMYFSVNMYSLPLIAVWIV
                     LFTSRLRTTMNWTTENFFPLAIGCALIYTSSNVIHNPALLPSVILTFGGATFFAAQKM
                     QEG"
BASE COUNT          843 a          773 c          793 g          981 t
ORIGIN      
        1 acatacctgt atcttgcttt tcttgaaata tacttcggat gaggcagggc atgaaaactt
       61 tcttccgtaa acggttggcc cagatactgc cgctcgtgct ttctgtgtac tgcgcagcag
      121 ttcagatcta tacagataac aagagcttgc atcttttctg cgacagagac atgtttatct
      181 tgattgatca ttacggcgag aagcgggtgt atttcgacaa tggcaaagaa gaattatttg
      241 ttggttatct tactgaatat agccttccag gaatgcgaac tttgtttgtc gaaatatcaa
      301 aaaccaaacg ctgtatgatt gggaaggtgg accttcccgg cgattttatt gatgtccgtg
      361 taaatggttt tggacttcac gatacccaaa aaattcccct cgatgatggt tactttatta
      421 tgcctatcat cggagttgat gtttacggca ttactgcttt cgacctaaca gtcaatgaca
      481 acggcaaccg gcatgatgtt tcaaaaaaat tcgatccgcc tttttctccg tattattttt
      541 cagttgcgga tattgcagaa gattatgagt ggtcacgagc aaaatcgaga tcggctttgc
      601 cgtagatttt gttttgatac gaagtggaga gtgacgctta ccgcgttatc ccttcaggat
      661 actgcgtgaa accgggcgaa aaggaagcca accaacggga gtgatcccgc tgctcgttcc
      721 tgtcctgttg tcccaatgct ttgggcagga aggggaagga ataagttccc cccgaccaga
      781 cgctaaaaag cagcaggagc gcaacgcgct aaacttggta ccggcttcgg ctggaaccag
      841 acggcgtgcc actatctgtt taccattatg gtttactatg gcaaggataa gacagagatg
      901 cagttttccg ggaagcgaca ttgtcaatct gacactgtcc tgtgttcgta tgagcatccc
      961 ccttcgggga tgtgggcaaa acccacatcc tttcttgaca agatagcacc ctcctgtatt
     1021 ctaggagggt gctgtttttc ttaaaaggtg gaaaggtcgt ccaatccatc aactgcctta
     1081 cgtttatctt cctcttttga tgatccgtag cgtaaagcca tttccaagga gtgacccatc
     1141 agggcagcgg cagttttggg atgtgtgccg ttcttcatca acatgtaggc ataggtgtga
     1201 cgcaggatat gaggagtaac ttccacgccc gaacgcttcg aagcctcttc cagcatcaat
     1261 tgaacagtgc gctgggtaat ccggctgtgt cctcgcttaa gacctatgaa aagggcgtcg
     1321 tctgcagctg gaaactccct acgaacatcc agccaatctg cgattgcttc acgcacttgg
     1381 cgaggaatat caacaactct tggctttcct ccctttccat cacgaatgat gatattaccg
     1441 ctgcgttctt ttatggtcac atcgctcacg tccaatccga tcagttcgct aatccgcggg
     1501 ccagcgtaca tcatggtaag catcatggca cgatctcgga taagcatttt tggagcaagt
     1561 gtcttttctc ttcgacggct ggtcttattg gcagtgcgtt cccaaggtgt gcgaaaaaca
     1621 tactccaacc ttgctcgttg atcatcatct agtccgcgtg gatcggacgg atctggtttt
     1681 acattcttga ttttctccgc tgggtttacc ggcacgatgc cactggaaag taggaatgtc
     1741 ccgaagacac gcattgcggc gccatgtcgc gcaatagtgt tgagtgcata tttttcggcg
     1801 tgtaaatgtt tgaggtatgc atcgatgacc gctttattca gaaggtgagg ctgacctttc
     1861 aggagtgatc tgtttaattc aattcctttg tgtccctttg cccatccaat gaatgctcgg
     1921 atatcgctta ggtatccctt caccgaagtg ggggagttgt tgtcgttcag atagtgttca
     1981 aaatgttcga gtaagtcgtt ttccaagttg caccttcttc tctatacaaa ggcggcgatg
     2041 atcgcgataa ctgttccaac aatgacgaca ataacaatca ctgctgcgat aacgcctcct
     2101 cgtatttctg atcgacgacg tgtatggatt gcccgtagta atgccacagc ttccttacgc
     2161 ccggtttcat ctgcgcgtgt tctcaattct gcaacaaggt tcgggagtgc tttggtacgt
     2221 tctgcccagg caatccaata ttcaaagtct ttatcaatct gtttcttatt attcatattc
     2281 ttctcctttc ctggacagcc ccttgtacac atagaatata cctttaccgc caaacagcac
     2341 gacaagcaaa atgcctgctt ttgcaatggg gtggacactg gtgaacataa cgatcagcgc
     2401 aacgagtgcc atgatcaatt ctgtaattcc gatgaatagt ttcatagttt tcattgtttt
     2461 tccttaataa agtctgtttc gcatagtcat tatcttatga ttgtattgga ctccattgca
     2521 atttcaggaa ttcttcaacc aaccttttat caccgacata aagattatta gatttccgat
     2581 cgacgagcag gtagttagtg ggcaaggtgt cactgtcacc gatccgatgt ttattgattt
     2641 gatgcagcga ggcgaacttg agaaagtatt gacactcggc tccgcacaag gagcgccgtc
     2701 catcgagcca ggtcaattca tctcctgcat ggtcccagta gaaaccaacg tagcgggcgt
     2761 cgctgttgta tccgaaagct tctgctaggt catcagggta atgaacagtc atcagcaagt
     2821 cgaagtcatc atgcatgatt cattatcctt cctgcatctt ttgcgcggca aagaatgtgg
     2881 cgccgccaaa ggttaggatt acacttggaa gcagcgcggg gttgtgtatg acattgctgc
     2941 tggtgtaaat caaggcgcag ccgatggcga gagggaagaa attttccgtg gtccaattca
     3001 tggttgtgcg taaacgggat gtgaacaaaa cgatccagac ggcaatcaat ggcagcgaat
     3061 acatattcac agagaagtac atctcaatcg cgccgatgtc gcccagaact gcaatgagcg
     3121 cgagaatgat cagcaaaacg tagttgggtc cagtcatcat ctgcatcctc gcataaaacc
     3181 gtaaagacgg taggtgttgg taggggtatt cataaatagt gggttccttt ttgtaaccga
     3241 gttatacgaa gtaaatttac atacttattg ggtcatttga actcggttta ttgcttaaat
     3301 atacggtttt acttcgtaca agtcaagttg tacgaagtaa atttgcgcga tttttgaggc
     3361 ttccccgcag ccttcttttc gattttttat
//
LOCUS       DAOQJE010000326         3382 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_18083_length_3382_cov_5.008717, whole genome shotgun sequence.
ACCESSION   DAOQJE010000326 DAOQJE010000000
VERSION     DAOQJE010000326.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3382)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3382)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3382
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18083_length_3382_cov_5.008717"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..688
                     /locus_tag="PLT08_09420"
     CDS             <1..688
                     /locus_tag="PLT08_09420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE04725.1"
                     /translation="TVSPSAFEAENISTFNELGIAIGFNYPEGFSQSISTSFNTTYSP
                     VAPYDLPYPQNAQIIFTDYSGGDGFTINGLRVFLADEVNALETGAVESVNAVLEGEGD
                     HHVDFPRLAGAGSIIDAQVLPLAFQNGNGYRYLVAKSFSADPISSTKLTYMYQGVTND
                     GKFFVSFIMLVDAPFLSEYVGQTLTTPEEFKTYFQDVNNLVETSSGDQFTPSLNALDE
                     LISSIVILEK"
     gene            698..1861
                     /locus_tag="PLT08_09425"
     CDS             698..1861
                     /locus_tag="PLT08_09425"
                     /EC_number="1.13.11.5"
                     /inference="COORDINATES: protein motif:HMM:NF016125.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004411 - homogentisate 1,2-dioxygenase
                     activity [Evidence IEA];
                     GO_process: GO:0006559 - L-phenylalanine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006570 - tyrosine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homogentisate 1,2-dioxygenase"
                     /protein_id="HNE04726.1"
                     /translation="MPFYHKLGKIPHKRHTQFKKPDGKLYREELMGLEGFSSLQSILY
                     HHFLPPRVKKTADLGSAKPEYVDYGAIRHRAFKTAPTPLGADPVSGRIPLLGNNDVIL
                     GVSRAPKGKMNFFYRNSQAYETWWVHDGSGTIYSQFGKLPFRKGDYIVIPFGTTWQME
                     LDEETKFFTIENPSQIEPPKRYRNHYGQLLEHAPYCERDIRIPMELETHTERGEFEVR
                     VKVRDHISQHILDYHPHDVIGWDGYLYPWIFNIEDFEPITGRIHQPPPVHQTFEGWNF
                     VVCSFVPRLFDYHPEGIPAPYNHSNIQSDEVIYYAEGNFMSRKGIDRSDISLHPFGLP
                     HGPQPGMTEASIGKTETKELAVMVDTFHPLHLTKQALEMEKPYMETWLDGDGE"
     gene            1886..2587
                     /locus_tag="PLT08_09430"
     CDS             1886..2587
                     /locus_tag="PLT08_09430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0070064 - proline-rich region binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HNE04727.1"
                     /translation="MKRSIYLFLITSLLLTSCSMSSSDAPSPEVATAAAMTVQAAIEG
                     NQTPLASPTSAGQSTPESMGNPMASFEDVTNCRVGPGVNYERVTQIQPGFAVEIVGYY
                     PPNYWIVQTDKGPCWVAGEFVTPSGSVSAVPTVTAPPTPTGGAPENVSLQKWDVACDY
                     ATNEAKVTIAWKDEDDEIGYRVVRNDVVIAELPANTTEFKETITLISGQTAGYSIISF
                     NATGSTQSQTISLGC"
     gene            complement(2634..>3382)
                     /locus_tag="PLT08_09435"
     CDS             complement(2634..>3382)
                     /locus_tag="PLT08_09435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HNE04728.1"
                     /translation="LHLGILIETMRREGYEFQVSRPEVIYHKADDGVLLEPYEEVHIE
                     TSNETVGPVVEMLGARRGRMMDMQDSGQGTTRMVYIVPTRGLLGFRYQFLTSTRGAGV
                     MNTIFHSYDELAGAMNTRQSGSLVAWEPGTTTAYALKSAEERGTLFVGPGVDVYEGMV
                     VGENARAADISVNVCKKKHLTNMRASRADMEIRLTPPRQMSLDEAIEYLADDELLEVT
                     PATFRIRKRILNTEERGKQTKKAKEQFGEE"
BASE COUNT          916 a          839 c          756 g          871 t
ORIGIN      
        1 aacagtttca ccgtctgcgt ttgaggcgga aaatatttcc acgtttaatg agttgggcat
       61 cgcaatcggt tttaattatc ccgagggctt ctcacaaagc atcagcacga gcttcaacac
      121 aacctattcg ccggtagctc cttacgacct gccatatcct caaaatgctc agatcatctt
      181 caccgattat tcaggtggag atggtttcac aataaatgga ctgcgtgttt ttctggcaga
      241 tgaagttaac gctttggaaa ccggagccgt ggagagtgtg aatgcggtcc ttgaaggtga
      301 aggagatcac catgttgact ttccgcgctt ggcgggtgcg ggcagcatta tcgatgcgca
      361 agtgctccct ctggcattcc aaaatggcaa tggctatcgc tatcttgtgg caaagtcttt
      421 ctcagcagac ccaattagca gcacgaagtt aacttatatg tatcagggcg ttacaaatga
      481 tggaaagttt tttgtgtcgt tcatcatgct agttgatgca ccttttctta gtgaatatgt
      541 tgggcaaact cttacaacac ccgaagagtt taaaacttat tttcaagatg tgaataacct
      601 tgtagaaacc tccagcggcg accaatttac tccttcactt aacgcgctgg atgaattgat
      661 ttcttcgatt gtcattttag aaaaataagg agaagacatg ccgttctatc acaaacttgg
      721 gaaaattccg cacaaacgtc acacacaatt caaaaaacca gatggaaaac tttatcgcga
      781 agagctgatg gggctcgaag gattttcatc gctgcaatcg atcctctatc atcactttct
      841 gcccccgcgc gtaaaaaaga cagcggatct tgggtctgcc aaacctgaat atgtggatta
      901 cggagcgatc cgtcatcgtg cgttcaaaac ggctcccact ccgctcgggg cagatccagt
      961 ttctggaaga attcctctgc tcggaaataa cgatgttatc ttgggagtct cacgggctcc
     1021 taaaggaaaa atgaactttt tctaccgcaa ctcacaagct tatgaaactt ggtgggttca
     1081 tgatggtagc ggcaccatat attcccaatt tggcaaatta ccctttcgaa aaggcgatta
     1141 catcgtgatt ccatttggca caacttggca aatggaatta gacgaagaaa caaagttctt
     1201 caccatcgaa aaccctagcc agatcgagcc gcccaagcgc taccgcaatc actacggtca
     1261 actgctcgaa catgcccctt attgtgagcg tgatatccgc atccccatgg agttagaaac
     1321 gcacaccgaa cgtggtgagt ttgaagtgcg tgtcaaagta cgcgaccaca tctcgcaaca
     1381 tatcctagac tatcatcctc atgatgtcat tggctgggat ggatacttat acccttggat
     1441 tttcaacatt gaagatttcg agcctattac cgggcgtata catcaaccac cgccagttca
     1501 ccaaaccttt gaaggctgga atttcgttgt atgttctttt gtgccacgtc tctttgatta
     1561 tcaccccgaa ggcattcctg cgccatacaa tcattccaac atccaatctg acgaagtcat
     1621 ctactacgca gaaggcaatt tcatgtctcg caaaggcata gaccggtctg atatcagttt
     1681 acatccattt gggttgccgc atggtccaca acccggtatg acagaagcca gcattggaaa
     1741 gacagagacg aaagagctcg ccgtaatggt cgatactttc catccgcttc atttgaccaa
     1801 acaagctctc gaaatggaaa agccgtatat ggaaacctgg ttagatggtg acggcgaata
     1861 aagataattt acatcggaga atacaatgaa acgatcaatt tatttattcc tcataaccag
     1921 tttgctcctc acatcttgta gcatgtctag ttctgatgcc ccttccccag aagtagcaac
     1981 tgccgctgct atgacagttc aagccgctat tgaaggtaat caaaccccgc ttgccagccc
     2041 aaccagcgca ggacagtcca caccagaaag catgggaaac cctatggcaa gctttgaaga
     2101 tgtcaccaat tgccgtgtgg gacccggtgt aaattacgag cgtgtcaccc agattcagcc
     2161 agggtttgcg gtagaaatcg tgggctatta cccgcctaat tactggatcg ttcaaacaga
     2221 caaaggacct tgttgggtag ctggcgaatt cgtcacccca tcagggagtg ttagcgcggt
     2281 tccaacggtt acggcaccac caacacccac cggtggagca ccagaaaatg tcagcttgca
     2341 aaaatgggat gtcgcttgtg attacgctac aaatgaagcc aaagtaacga ttgcgtggaa
     2401 agatgaagat gatgaaattg gctatcgcgt cgttcgcaat gatgttgtga tcgctgagtt
     2461 acccgcaaat acaacagagt ttaaagagac catcacgcta atctcaggtc aaacagctgg
     2521 gtatagcatt atctccttca atgcaacagg gtctacgcaa agtcagacaa tctcactagg
     2581 ctgttagttt gtgaatcaaa aagagccatg caaattgcat ggctctttta tttttattct
     2641 tcgccgaact gttcttttgc cttcttcgtt tgcttgccgc gctcttcggt gttcaaaatg
     2701 cgcttgcgaa tgcggaatgt agcaggcgtc acttcgagca attcatcgtc agcgaggtat
     2761 tcaatcgctt cgtcgagaga catttgtcgg ggcggagtga ggcgaatttc catatccgca
     2821 cgtgaggcgc gcatattcgt caaatgcttc tttttacaga cattgacaga gatatctgcg
     2881 gcgcgggcat tttcgcccac caccatgcct tcgtaaacat caacgcccgg tccaacgaac
     2941 aaggtgccgc gttcttcagc agacttcaat gcataagccg tagtcgtgcc aggctcccat
     3001 gctaccaacg aaccactctg acgagtattc atagctccgg caagttcatc atagctatgg
     3061 aaaatggtgt tcatgacacc tgcaccacga gtggacgtca ggaattgata gcggaagccg
     3121 agcaaaccgc gggtcggcac gatgtacacc atacgggtcg ttccctgacc tgaatcctgc
     3181 atatccatca ttctgccgcg tcttgcaccg agcatttcca ctacgggtcc gacggtttca
     3241 ttgctggtct caatatgaac ttcttcatac ggttcaagca aaacaccgtc atctgccttg
     3301 tgatagatta cttctgggcg agagacctga aattcataac cttcacgacg catggtttca
     3361 ataagaatcc ccaagtgcaa ct
//
LOCUS       DAOQJE010000327         3375 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_18150_length_3375_cov_8.321988, whole genome shotgun sequence.
ACCESSION   DAOQJE010000327 DAOQJE010000000
VERSION     DAOQJE010000327.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3375)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3375)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3375
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18150_length_3375_cov_8.321988"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..971
                     /locus_tag="PLT08_09440"
     CDS             <1..971
                     /locus_tag="PLT08_09440"
                     /inference="COORDINATES: protein motif:HMM:NF024778.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HNE04729.1"
                     /translation="LASSLAYQIEQDYSAQAKNKKATRTNSAMPIALFLIAKLVAYTL
                     MGALLGWLGSFLTLSPLTRAILMIAIGIFMVGNALRMFNVHPIFRYFSIEPPKFITRY
                     IRRTAKGTDTATPIFLGLLTIFIPCGVTQAMMATALGTGSVLMGAALMFAFTLGTSPV
                     FFIVAYLATELGAKLEKFFMRFVAVVVLILGLVTLDGGLNLMGSPLSLQNMTRGWFAP
                     QAESNAITESVQPSAASDEIILSVENNGYFPAILKAPAGKDLTLSLVTNKTYSCARDF
                     VIPDLNFYELLPDTGTVQVNIPAQKAGSTLFFTCSMGMYTGQIVFE"
     gene            1005..1217
                     /locus_tag="PLT08_09445"
     CDS             1005..1217
                     /locus_tag="PLT08_09445"
                     /inference="COORDINATES: protein motif:HMM:NF012621.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HNE04730.1"
                     /translation="MIKKTFKIPDMSCTNCAMKIESLEDDLDGVKEVNASYHKLEMVI
                     EYDEAKLTDEQIVTAVKKKGYTAVAA"
     gene            1352..1735
                     /locus_tag="PLT08_09450"
     CDS             1352..1735
                     /locus_tag="PLT08_09450"
                     /inference="COORDINATES: protein motif:HMM:NF021486.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2203 domain-containing protein"
                     /protein_id="HNE04731.1"
                     /translation="MPKYYTPKEANNLLEIVRPMVGDLMQIGERVRARGPEIWAMVEK
                     SAGNGGNPELSKMLPDFDRLDEILHRLQDMEIEVKDLASGLIDFPALRDGTVVYLCWK
                     YGEGSIQFWHEIEAGFAGRQPIDWE"
     gene            1775..2605
                     /locus_tag="PLT08_09455"
     CDS             1775..2605
                     /locus_tag="PLT08_09455"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HNE04732.1"
                     /translation="MSASTIGRYEIESELATGGMGVIILAKDPYIQRQVVVKVLMYSR
                     TLDTVYRDFFQNEAEVIAALEHPCIVPIYDFGWHGQQPYIVMRYMAGGSLQDHLEKGE
                     IKLTEMAHIFKRVADALDAAHAKSIIHRDIKPSNILFDASGEAFLSDFGIATSKSIKD
                     DDGEWLVGTPAYMSPEQAKGGHVDGRSDVYALGVTLYRLLTGQLPFSSDSATSLINSH
                     AELPIPDIRSVKSNIPAVWQEVVSKAMAKDPKDRYATAGDFARDVNEVVSGKWFLRKL
                     "
     gene            2699..>3375
                     /gene="rarD"
                     /locus_tag="PLT08_09460"
     CDS             2699..>3375
                     /gene="rarD"
                     /locus_tag="PLT08_09460"
                     /inference="COORDINATES: protein motif:HMM:TIGR00688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter RarD"
                     /protein_id="HNE04733.1"
                     /translation="MKKGIWYGIAAYAMWGFFPIYWKLLHDIPALQLLGHRISWSFLL
                     LFAFIFITKQWDEFRAVAFNRRTLGIYAVAGVLLSLNWLIYVWGVNAGFIVETSLGYF
                     INPLLSVLFGVIFLREKLRPMQWLPVVIATIGVTYLTITYGRLPWIALSLAVSFGLYG
                     LVKKLSPLGSVFGLTLETGIVFPVALIYLAFVQVNGTAGFLHDGALVDILLIGGGIVT
                     TIPLLMFA"
BASE COUNT          819 a          809 c          812 g          935 t
ORIGIN      
        1 tgcttgccag ttcacttgcc tatcagatcg agcaggatta cagcgcacaa gccaaaaata
       61 aaaaagccac gcgtacaaat tctgccatgc cgatcgcact ctttcttatt gccaaactcg
      121 ttgcatatac tttgatgggg gcgttgctcg gctggctggg ttctttcctc accttaagtc
      181 cgctcacgcg cgccatcctg atgatcgcca tcggcatttt catggtgggt aacgcgttgc
      241 gcatgttcaa cgtccacccg atctttcgtt acttttccat tgagccgccg aagttcatca
      301 cacgatatat ccgccgaact gccaaaggca cagataccgc cacacccatc ttcctcggct
      361 tgctcacgat cttcattcct tgtggcgtca ctcaagctat gatggcaacc gctctcggca
      421 cagggagtgt acttatgggt gcagcattga tgttcgcctt cacccttggc accagcccgg
      481 tatttttcat cgttgcatat ctcgccacag agctcggcgc aaaacttgaa aaattcttca
      541 tgcgatttgt cgccgtggtg gttttgatct tgggactcgt cacccttgat ggtggactca
      601 acttaatggg ctcgcccctc tcgcttcaaa acatgactcg cggctggttc gccccacaag
      661 cagaatccaa cgctataaca gaatccgtcc agccctctgc cgcaagtgat gaaattatcc
      721 taagcgtaga gaacaacggg tatttccctg ccattctcaa agctccagca ggcaaagacc
      781 tgaccttaag cctcgtcacg aataaaacct attcctgtgc ccgcgacttt gttattcccg
      841 atttaaattt ctacgaattg cttcccgata caggtactgt tcaagtgaac atcccagcgc
      901 agaaagccgg ctcaaccttg ttcttcactt gctccatggg tatgtacacc gggcagattg
      961 tgttcgaatg ataaacgtag ggtcgcccct acattggaga tgaaatgatt aagaaaacct
     1021 tcaaaatccc cgatatgagt tgtaccaact gcgccatgaa gatcgagtct ctggaagatg
     1081 acctggatgg cgtgaaagag gtcaacgcca gctatcacaa attggagatg gtcatcgagt
     1141 acgatgaggc gaaactaacg gatgagcaaa tcgtgaccgc tgtaaaaaag aagggctata
     1201 ccgcagtcgc cgcataagtt gaccttttaa aaggcattat caaggcatgt ggagcaataa
     1261 atgtggacaa cccatttaag ctccacatgc ctacttaatt taaagatttt gtttgttttc
     1321 attttgaccc ctttccgtta tactcgcgcc catgcccaag tattacacgc ccaaagaagc
     1381 caataatctg ttggaaatcg tccgaccaat ggtaggagat ttgatgcaga tcggggaacg
     1441 ggtccgcgct cgtggacctg agatttgggc gatggttgaa aaatctgcgg ggaacggcgg
     1501 caaccccgag ctaagcaaga tgctgcccga ttttgaccgt ctcgatgaga tcctgcatcg
     1561 ccttcaggat atggagatcg aggtgaaaga cctagcttca gggttgatcg attttccagc
     1621 gttgcgagat ggcacggtcg tttatctgtg ttggaaatat ggagagggga gtatccagtt
     1681 ttggcacgag atcgaggcgg ggtttgcggg gcgtcaaccg attgattggg aataatccga
     1741 acgctaaaaa ttcattgtat cccggagaga agagatgagt gcatccacaa ttggacgtta
     1801 tgagatcgaa tctgaattag ccacaggggg catgggggtt atcatccttg cgaaagaccc
     1861 atacattcag agacaggtcg ttgtcaaagt gttgatgtat agtcgcacct tggatacggt
     1921 ctaccgcgat tttttccaga atgaagcgga agtcattgct gcgttggaac atccctgcat
     1981 tgtgcccatt tatgattttg gctggcatgg acaacaaccg tatatcgtga tgcgttatat
     2041 ggcgggcggc agtttacaag accaccttga aaaaggcgag atcaaactga cggaaatggc
     2101 gcatatcttt aagcgtgtgg cagatgcgct tgatgctgca catgcaaagt ccattattca
     2161 tcgcgatatc aagccatcca acattttgtt cgatgcctct ggtgaagcct tcctctctga
     2221 ttttgggatt gcaacatcaa aatccatcaa ggatgatgat ggcgaatggt tggtgggcac
     2281 acctgcatat atgagccctg aacaagccaa aggcgggcat gtagatggac gttcagatgt
     2341 ctatgcatta ggtgtaacgt tgtatcgcct tctgaccgga cagttgccat tttccagtga
     2401 ttcagcgacc agcctgatta actctcatgc agagctaccg atccctgata ttcgcagtgt
     2461 taaatcaaat attcctgctg tgtggcaaga agtcgtttcc aaagccatgg cgaaagaccc
     2521 caaagatcgt tatgccaccg ctggcgattt tgctcgagat gtgaacgaag tcgtctctgg
     2581 aaaatggttc ttgaggaaat tgtaggaaaa tttctttcaa acgcatctac aagaaaataa
     2641 agtgtttttc tttataagag cctgttaagg ctctatattg ttttacgtta ggagaaaaat
     2701 gaaaaaaggt atttggtacg gcatcgcagc gtatgcgatg tggggtttct tccctatcta
     2761 ttggaaattg ttacatgaca ttcctgcttt gcaattgctt gggcatcgta tttcttggtc
     2821 attcctgctc ttattcgcat tcattttcat caccaaacaa tgggacgaat tccgcgctgt
     2881 tgcattcaac cgcagaaccc ttggcatcta tgccgtagca ggcgttttgc tttctttaaa
     2941 ctggctaatc tacgtgtggg gcgtgaacgc tggcttcatt gttgaaacca gccttgggta
     3001 tttcatcaac ccgctattaa gcgtgctctt tggcgtgatc ttcttgcgcg aaaaactacg
     3061 tcccatgcaa tggttgccgg ttgtgattgc taccatcggt gtgacttatc tcaccatcac
     3121 atacggacgc ttgccctgga ttgcgctttc attggcggtt agctttggtt tgtatggctt
     3181 ggtcaagaaa ctttcgccgc tcggttcggt cttcgggttg actctcgaaa caggcattgt
     3241 cttccctgtt gccttgatct accttgcttt tgttcaagtg aacggcactg ctggcttctt
     3301 gcatgatggc gctctcgttg atattctttt gatcggcggc ggcattgtca ccaccattcc
     3361 gcttttgatg tttgc
//
LOCUS       DAOQJE010000328         3375 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_18151_length_3375_cov_4.691867, whole genome shotgun sequence.
ACCESSION   DAOQJE010000328 DAOQJE010000000
VERSION     DAOQJE010000328.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3375)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3375)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3375
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18151_length_3375_cov_4.691867"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1365
                     /locus_tag="PLT08_09465"
     CDS             <1..1365
                     /locus_tag="PLT08_09465"
                     /inference="COORDINATES: protein motif:HMM:NF013188.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HNE04734.1"
                     /translation="RFGKFVFQKLTGRAVEFQFVIVALFLAAFFAEVIGVHEVVGAFL
                     AGLAVNSMLPRHSPVTGHVLFIGESFFIPVFLLYSGLITDPLTFLKSPQTIIVAVGVT
                     IVAYVSKLIAAWLTARIYKYTRSEFWTVYGLSHAQAAVTIPTLVIGLETGLFDATLFN
                     AAILMILLTSITSPLIVQRFAPDLQTASTDDEQTPLFGRILVPVSEAHHSEGLVALSS
                     LLARSSKGKVLAVSVAQDKGPNDNSSLMLHKELLGKVSANLHDPEAELELIPRLAATH
                     AQGILHTANEENASLIVMGWRGKRTFRESVLGSVLDEVIWGSDTPVVAGKLSLPLNSM
                     QRVIFIVPAKAVPPIALRRMLEANLAIAKALNVPLLVRADKSYVQTLEALFVAISPEQ
                     EYKLEVLMDQLKPERLEHEAVSDFIILPGFGSRKRVQDTLGNTPEQIAKYFDGNLALL
                     HFDK"
     gene            complement(1431..2495)
                     /locus_tag="PLT08_09470"
     CDS             complement(1431..2495)
                     /locus_tag="PLT08_09470"
                     /inference="COORDINATES: protein motif:HMM:NF012546.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HNE04735.1"
                     /translation="MKKTLVVFLAFLVAITACTEATPMPTHTPIPVTDTPSQTSTLEP
                     TATFTPAPTATPVPHPMSIIALRNQEYPGSEITVVKELDRGINYRRYYVYYLSEGLKI
                     YALLTIPNGEKPEEGFPAIVFNHGYIPPDVYKTTERYIAYVDQLARAGYVVFRIDYRG
                     HDQSEGEATGAYGDPGYQVDVMNALASLKQHPDVNPEKIGMWGHSMGGYLTLRAMVIS
                     NDVKAGVIWAGVVASYSDLLYNWRRTGAFTPSPSSTGRGWRSAWIEQYGTPEQNPAFW
                     DSISATSYLTDLSGPLELHHGTADEDVPVEFSIRLAELGRQSGQIVNLYTYEGDNHNI
                     SNYFTQAMNQTIAFFDIYLK"
     gene            complement(2603..>3375)
                     /gene="mmuM"
                     /locus_tag="PLT08_09475"
     CDS             complement(2603..>3375)
                     /gene="mmuM"
                     /locus_tag="PLT08_09475"
                     /EC_number="2.1.1.10"
                     /inference="COORDINATES: protein motif:HMM:NF007020.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047150 - betaine-homocysteine
                     S-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="homocysteine S-methyltransferase"
                     /protein_id="HNE04736.1"
                     /translation="FNAGADIAITASYQATVDGFAKRGLSREQALDLMKKSVLLAQEA
                     RDEFWAKEENRVGRIRPLIAGSVGPYGAYLADGSEYRGDYNLSEDELIAFHRPRVEAL
                     IASGADLLACETIPCGIEARALIRLLAEIPNASAWFTFTAKDGEHISNGERIADIAAF
                     LDAKPQAAAIGINCTSPLYIPALVREIKKVTSKPIIVYPNSGEYYSAVTNSWHGETSS
                     DKFGLQSQEWFENGATLIGGCCRTTPGHIHEVSNWARK"
BASE COUNT          771 a          854 c          862 g          888 t
ORIGIN      
        1 cgctttggaa aattcgtctt tcaaaaactt accggtcgcg ctgttgaatt tcaattcgtg
       61 atcgtcgcct tatttttggc ggcatttttt gccgaagtga tcggcgttca cgaagtggta
      121 ggtgccttcc ttgcaggctt ggcggtcaac tcgatgttgc cacgtcatag cccagtgaca
      181 gggcatgtcc tcttcatcgg cgagtcattt ttcatccccg tctttttgct ttatagcggt
      241 ctcattaccg acccgctgac cttcctcaaa agtccgcaga ccattattgt tgccgtcggc
      301 gtcactattg tggcgtatgt ttcaaaacta attgctgcct ggctcactgc ccgaatttat
      361 aaatacacca gatccgaatt ctggaccgtc tatggcttgt ctcacgccca agctgccgta
      421 accatcccta cgttggtcat tggtttagaa actggacttt tcgacgccac cctcttcaat
      481 gccgccattt tgatgattct cctcacctcg atcacttcgc ctcttatcgt ccagcgcttc
      541 gcccctgacc ttcaaactgc ttctaccgac gatgagcaga cgccactctt cgggcggatt
      601 ctcgtccctg tttctgaggc gcatcattcc gaagggttgg tggctctttc cagcttatta
      661 gcccgcagtt caaagggcaa ggtattggca gtgagtgtgg cgcaagataa aggtccaaat
      721 gacaatagca gcctgatgtt gcataaggaa ctgctcggaa aggtcagtgc taatttacac
      781 gaccccgaag cagaacttga attgatccca cgccttgcag caacgcatgc acaaggtatt
      841 ttgcacaccg ccaacgaaga gaatgcctcc ctaatcgtca tggggtggcg cggcaagcgc
      901 accttccgtg agagtgtgct cggttctgtt ctggatgaag ttatctgggg ttctgataca
      961 cccgtggtgg ctgggaagct atctttgccg ctaaatagta tgcagcgcgt aatcttcatc
     1021 gtgcctgcca aagctgtccc gccgatcgcg ttacgtcgca tgctcgaagc caaccttgcc
     1081 attgccaagg cgcttaatgt gcccttgctc gtccgggcag ataagagcta tgtacagacg
     1141 ctggaggcat tgttcgtcgc catcagccct gagcaggaat ataagctcga agttctgatg
     1201 gatcaactta agccggaacg cttggagcac gaagccgtca gtgactttat cattttgccg
     1261 gggtttggct cccgcaagcg cgtgcaagat acccttggaa acacccctga gcaaatcgct
     1321 aaatattttg acggaaattt agcactcctg catttcgata agtaaattat ctgatcatgg
     1381 acgatgggca ataaaccgcg atcaacggtc tattgcccat cgtcttgttt ttacttcagg
     1441 taaatatcga agaatgcaat cgtctgattc atggcttggg taaaatagtt cgaaatgttg
     1501 tgattatcac cttcgtatgt gtacaaatta acgatttgac ctgattgcct gcccaactct
     1561 gccagccgaa tcgaaaactc taccggcaca tcttcatctg cagtgccatg atgcaattcc
     1621 aacggaccgg atagatcagt aaggtaggat gtcgcagaga ttgagtccca gaaggcgggg
     1681 ttttgctcgg gtgtgccgta ttgttcgatc catgctgatc gccatccacg cccggtagag
     1741 ctaggtgacg gggtgaaggc acccgtccgg cgccagttat acaacaggtc tgagtatgaa
     1801 gcgacgaccc ccgcccagat cacaccggct tttacatcat tcgagatgac catggcgcgc
     1861 agggttaaat atccgcccat ggaatgcccc cacataccga tcttctcagg gtttacatct
     1921 gggtgctgtt tgagagaggc gagggcattc atcacatcta cttggtagcc ggggtcgcca
     1981 taggcaccag tggcttcccc ttcggactgg tcgtgcccgc gatagtcaat tcggaagacc
     2041 acataccctg cccgagcgag ttgatcgaca tatgcaatat agcgctctgt tgttttgtat
     2101 acatctggcg gaatgtaacc atgattgaag acgatggcag ggaacccttc ttcaggtttt
     2161 tcaccattcg gaatggtgag caaggcgtaa atcttcaacc cttctgaaag atagtaaaca
     2221 taatatcggc gataattaat gccgcggtct agctctttga cgacggtgat ttcgctgccg
     2281 ggatattcct gatttcgcaa cgcgatgatg ctcatgggat gtggaacggg cgtggctgtc
     2341 ggagccggtg tgaatgtagc tgtgggctcg agtgtggagg tttgtgaggg cgtatccgtt
     2401 actgggattg gcgtatgggt aggcatggga gtcgcttcgg tgcaggctgt gatcgccacc
     2461 aaaaacgcga ggaataccac taaagttttc ttcattcttt ctcctttaaa tttttcactc
     2521 aacgtgatgt agaacaaaaa agagacggtc cattaccgtc tctttataca tggtttgtat
     2581 cagtaaaagt taagaaagaa ttttattttc ttgcccaatt gctcacttcg tgaatatgcc
     2641 ccggtgtggt gcggcaacat ccgccgatga gggtagcacc attctcgaac cattcttggg
     2701 attgcaaacc aaatttgtcg ctagaagttt cgccgtgcca gctattggtc accgcgctgt
     2761 agtactcgcc agagttcgga tagacaataa tgggcttgct ggtgaccttc ttgatttcac
     2821 gcactaacgc agggatgtat aagggcgagg tgcagttgat gccaattgct gcggcttggg
     2881 gctttgcatc gagaaaagcg gcaatatctg cgatgcgttc accgttgctg atgtgttcac
     2941 cgtctttggc agtgaaggtg aaccaggcag aggcattcgg gatttcagcc aggaggcgga
     3001 tcaaagctcg ggcttcgatc ccgcatggga tggtttcgca ggctaatagg tccgcaccgg
     3061 aggcgatgag ggcttccacg cgcgggcgat gaaatgcgat cagctcatct tcactcaagt
     3121 tgtaatcacc gcgatattca gacccatcag caagataagc gccataaggt ccaaccgaac
     3181 cggcgatcaa tggacggatg cgtccaacgc gattttcttc ctttgcccaa aattcatcgc
     3241 gagcttcctg cgccaacagc acagattttt tcatgagatc tagcgcttgc tcgcgactca
     3301 agccacgttt ggcaaaccca tctaccgtgg cttgataact ggcagtgatg gcgatgtctg
     3361 cacctgcatt gaagt
//
LOCUS       DAOQJE010000329         3374 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_18158_length_3374_cov_5.257608, whole genome shotgun sequence.
ACCESSION   DAOQJE010000329 DAOQJE010000000
VERSION     DAOQJE010000329.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3374)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3374)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3374
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18158_length_3374_cov_5.257608"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(47..439)
                     /locus_tag="PLT08_09480"
     CDS             complement(47..439)
                     /locus_tag="PLT08_09480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE04737.1"
                     /translation="MAPKPNTNKWSDVQLAIAAISMTSVIAFWNMFAGPDKVKADQKA
                     AEQQADIIPPTVMPVVVEPTTVPSVTMPPIGYTILFGGKAPQPKVVVVQKPKGGGGGG
                     NADSGNGGGGGGGGNASASQPSTSTTSS"
     gene            complement(452..961)
                     /locus_tag="PLT08_09485"
     CDS             complement(452..961)
                     /locus_tag="PLT08_09485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE04738.1"
                     /translation="MRKSTLFVSAMLTVFLMATMFGVVSAYQQVVKNNDLASTQYSQK
                     ALSQPVSVEFAAPATATVVAPLVVTAEEATTIAVNFLNDPMVYSVEVVDYQGAPTYLV
                     TFSSGELVYVSSEGVVVANTQVQPVVITASNTTSRGQSNNSGSSSNNSSGSNQQHEEH
                     EHEEHEGDD"
     gene            1132..2496
                     /locus_tag="PLT08_09490"
     CDS             1132..2496
                     /locus_tag="PLT08_09490"
                     /inference="COORDINATES: protein motif:HMM:NF024919.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C39 family peptidase"
                     /protein_id="HNE04739.1"
                     /translation="MNRLKNIDKRWLILAVVVVIIGIVLFPPVRNRLSVQYELLRTRI
                     VYFFNPPSEAVFQPSGEEEPLTVETAVGTVRAEMLLTLTPEATSTPKATKTGPTAKPT
                     VTSTPVPGKVILPGVVYVDQHGRWNYCGPANLTMALKFWGWNGDRDDVAKVVKPGSPN
                     QNLDFVERGLPDKNVMPYELVDFVNLQTTEYRALSRYGGDMDLLKRLLAAGFPVIVEK
                     GYYERDYTGKVDWLGHYLFTTGYDDERGGFIVQDAYLKPGKDLLSKYDEYQDGWRSFN
                     YLFMVVYPAGRENEVMSILGPWADETWAANHALEIANKEVSQLTGNNLFFAYFNKGTS
                     HVAINQYSDAAVAYDKAFNQYSKWDTTKGNRPFRMMWYQTGPYFAYYYSARYQDVVDL
                     ANTTLTKTISKPTLEESLLWRGRAYYMIGDTDAAIKDYRAALEVHANWYPAVQALQEL
                     GLQP"
     gene            2496..>3374
                     /locus_tag="PLT08_09495"
     CDS             2496..>3374
                     /locus_tag="PLT08_09495"
                     /inference="COORDINATES: protein motif:HMM:NF024258.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease SbcCD subunit D"
                     /protein_id="HNE04740.1"
                     /translation="MKLLHFADAHIDMANYGRHDPSTGLPLRVLDFLKSLDTIVDTAI
                     ERKVDMVIFAGDAYKDRSPAPTFQREWGKRIMRLSQAKIPTLLLIGNHDISPSIGRAH
                     ALQEFKTLQVPYVKVLDAPELLKPADLWDLPLQVIAMPWVARSGFMAATGETDSTQAF
                     SRIEENISELITGWIEECDLSMPIVLTAHASIEGAKFGGERLVMLGNDLVLTGGLVKN
                     PKLNYVAMGHIHKPQDVNEGHQPPVIYPGSIERVDFGEAKEERFFVIADVEKGRNTEV
                     EWIQLTGVRKFIDRRAT"
BASE COUNT          836 a          753 c          849 g          936 t
ORIGIN      
        1 acgagcatgt cactgcccat cgcgcgaaag ggatggctgt aaaacattag gatgaagtgg
       61 tcgatgtaga aggctgactg gctgacgcgt ttccgccacc gccacctcct ccgccgtttc
      121 ctgaatcagc gttaccgccg cctccgcctc ctttcggctt ttgcacaaca acgactttgg
      181 gttgaggtgc ttttccgccg aataagatgg tgtatccaat agggggcatg gttacagatg
      241 ggacagtggt aggttctacg acaacgggca taacggtagg agggatgata tctgcctgtt
      301 gttctgctgc tttttgatcc gcttttactt tatctggacc tgcaaacata ttccaaaatg
      361 cgatgacgga cgtcatcgag attgcggcga ttgccagttg aacatcagac catttatttg
      421 tatttggttt gggtgccata ttgttttcct attaatcgtc gccttcgtgt tcttcatgtt
      481 cgtgctcttc atgttgttga ttgcttccgc ttgagttgtt tgaagaactt ccgctgttgt
      541 tcgattgacc tctgctggtg gtgttggatg cagtgatcac aacgggttgc acttgagtat
      601 tcgctaccac tacgccttca gaactgacat aaaccaattc acctgaactg aaagtcacca
      661 aataggtagg tgcgccctgg taatcaacaa cttccacgct gtataccatg gggtcattta
      721 agaaattgac ggcaatggtt gttgcttctt ctgccgttac aacaagtggc gcgacgactg
      781 ttgccgttgc tggggctgca aattcaacgc tgactggctg tgataaggct ttttgtgaat
      841 attgtgtaga agctaagtca ttgttcttaa cgacttgttg atacgcagaa accacgccga
      901 acatggtcgc cattagaaat acggtgagca tggctgaaac gaacaaagtg ctttttctca
      961 taatttactc cttacagatt gggtttcaac ctgttacttt gagtcaagtc gtttcagcag
     1021 ggaaaaggtt atgcagttaa atgagtttgg cattaaagga ctttgtagaa attgtcagta
     1081 tgacgaacgg gaagcaaaaa acggtctttg ttttgcttta taatcttttt tatgaataga
     1141 cttaaaaata ttgacaagcg ttggttgatt cttgcggttg ttgtggtcat tattggtatt
     1201 gtgctcttcc cacccgtgcg aaatcgactt tcagttcaat atgaactttt acgcacacgc
     1261 attgtctact tttttaatcc acctagtgaa gctgtttttc aacccagcgg tgaagaagaa
     1321 ccgctgacgg ttgaaaccgc agtcggaacc gtccgcgcag agatgttgtt gacgttaacc
     1381 cctgaagcga cctcaacccc gaaggcaacc aaaacaggtc caaccgccaa accgacggtt
     1441 acatcgacgc cggtgccggg gaaggtcatt ttgccgggtg tggtgtatgt agaccaacat
     1501 ggacgttgga attattgtgg tcctgctaat ttgacgatgg cattgaaatt ctgggggtgg
     1561 aatggcgatc gcgacgatgt cgccaaagtt gtcaagccgg gttcaccgaa tcaaaacctc
     1621 gattttgttg aacgtggttt gcccgataaa aatgtgatgc cctatgaatt ggtggacttt
     1681 gtcaatttgc agaccaccga ataccgtgcc ttgtcccgct atggtggtga catggatctg
     1741 ctcaaacgcc ttctagctgc tggtttccct gtgatcgtgg aaaagggata ttacgagcgc
     1801 gattacactg gcaaggtcga ttggcttggg cattatctgt tcacgacagg gtatgatgat
     1861 gaacgtggcg gcttcattgt gcaagatgct tatctcaaac ctggcaaaga cttgctgagc
     1921 aagtacgatg aatatcaaga tggctggcgt tcgttcaact acctcttcat ggtggtatac
     1981 cctgctgggc gtgaaaacga agtgatgtct attctcggtc cgtgggctga tgaaacctgg
     2041 gctgctaacc atgcacttga aattgccaat aaagaagttt ctcaattaac cggcaataat
     2101 cttttctttg cctatttcaa taaaggcacc agccatgttg ctatcaatca atattcagat
     2161 gcagccgtgg catatgataa agcgttcaat caatattcca aatgggatac caccaaaggc
     2221 aatcgcccat ttcgaatgat gtggtatcaa acgggtccgt actttgcgta ttattattct
     2281 gcccgttatc aagatgtggt ggaccttgcc aatacaacct tgactaaaac catctctaaa
     2341 cctacccttg aagagtcttt gctttggcgc gggcgagcgt attatatgat cggcgatacg
     2401 gatgccgcca tcaaagatta tcgcgccgca ttggaagtcc atgcaaattg gtaccctgct
     2461 gtacaggcat tacaagaatt aggattgcag ccataatgaa actattacat tttgccgatg
     2521 cccacattga catggcgaac tatgggcggc atgacccatc tacagggttg ccgctgcgtg
     2581 tgctggattt tctcaaatcg ttggatacca ttgtggatac cgccatcgaa cgcaaggtgg
     2641 atatggtcat ttttgcgggc gatgcttata aggatcgttc tccggctccg accttccaac
     2701 gggaatgggg caaacgcatc atgcgtttat ctcaagcgaa gattccgact ctcctactaa
     2761 tcggcaatca cgacatttcc ccgtccattg ggcgggctca cgccttgcaa gagtttaaaa
     2821 ccctgcaagt gccttatgta aaagtgttgg atgctcctga gttattaaag ccagccgatc
     2881 tatgggattt gccattgcaa gttattgcca tgccgtgggt ggcacgctct gggtttatgg
     2941 cagccactgg tgagacggat tccacgcagg cgttttcacg cattgaagaa aacattagtg
     3001 agctaatcac cggttggatc gaggaatgtg acctatctat gccgattgtg ttgacggcac
     3061 atgcttccat tgaaggcgca aagtttggcg gagaacgctt ggtaatgctt ggcaatgacc
     3121 tggtgttgac tggcggattg gtgaaaaacc cgaagctcaa ctatgtggca atgggtcata
     3181 tccacaagcc gcaagatgtg aacgaaggac atcaaccgcc cgtgatttac cccggctcca
     3241 ttgaacgtgt cgactttggg gaggcgaaag aagagcgctt ctttgtcatt gcagatgtgg
     3301 aaaaaggacg caacaccgaa gtagagtgga ttcaactcac aggggtgaga aagtttatag
     3361 accgccgggc aact
//
LOCUS       DAOQJE010000330         3372 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_18176_length_3372_cov_5.987941, whole genome shotgun sequence.
ACCESSION   DAOQJE010000330 DAOQJE010000000
VERSION     DAOQJE010000330.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3372)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3372)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3372
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18176_length_3372_cov_5.987941"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..295
                     /locus_tag="PLT08_09500"
     CDS             <1..295
                     /locus_tag="PLT08_09500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE04741.1"
                     /translation="QMPPTLIVFASSFWLMIGIATLLSIWQEKPWSKNLLLGAAAGYT
                     VWYWSERLIWQNPRPNIPFTIVINFACLIVIYFASKSLSREAYERNTENPAIE"
     gene            261..1811
                     /locus_tag="PLT08_09505"
     CDS             261..1811
                     /locus_tag="PLT08_09505"
                     /EC_number="6.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA carboxylase subunit beta"
                     /protein_id="HNE04742.1"
                     /translation="MSETLKIQQLNDLKAQSRLGGGQARIDAQHKKGRLTARERLDLL
                     LDRGSFREVDPFVVHRTTDFGLDEQKIMSDSVVTGWGTIDGRLVYVYSQDFTVFGGSL
                     GEVHAEKICKIMDMAMKNGAPVIGLNDSGGARIQEGVVALGGYADIFLRNTMASGVIP
                     QISAIMGPCAGGAVYSPALTDFIFMTRNTSYMFVTGPDVVKAVTHEEVTQEELGGASV
                     HSEKSGVCHVAADSEADTLYLIRKLMSYLPQNNMEDSPAAPTSDDPLRMEEALNSLVP
                     DDANKPYDIKEVIRLIVDDGNFFEIHENYAANIVVGFARLGGQSVGIVANQPAVLAGV
                     LDIDASEKGARFVRFCDAFNIPIITFEDVPGFLPGTFQEHHGIIRSGAKLLYAYCEAT
                     VPKLTVITRKAYGGAYCVMSSKHIRSDLNLAWPTSEIAVMGPDGAVNIIFRRELEAAE
                     DPVKRKAELVAEYKEKFANPYVAASRGYIDDVIEPKETRPRLINALAMLSNKRDSNPA
                     KKHGNIPL"
     gene            1830..3338
                     /gene="accC"
                     /locus_tag="PLT08_09510"
     CDS             1830..3338
                     /gene="accC"
                     /locus_tag="PLT08_09510"
                     /EC_number="6.3.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003056649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0004075 - biotin carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HNE04743.1"
                     /translation="MFKKVLVANRGEIAVRILRACRELGLETVAVFSEADRNALHVRY
                     ADEAYLLGPAPSRESYLRTDKILDVVKRSGADAIHPGYGFLAEREDFAAKCADLGITF
                     IGPKPSAIAAMGDKAEARATVIKAGVPVVPGTEDVGNMTNDDLLRIAPTIGFPLLIKA
                     TAGGGGKGMREVRSLEEMPTLLESARREAESAFGDGNVYLEKLIEGARHIEFQIIADE
                     HGNVIHLGERECSIQRRHQKLVEEAPSPFLDEELRKQMGDVAVKAAQAVDYVNAGTIE
                     FLVDKDRNFYFLEMNTRLQVEHPVTELVTGIDIVAEQLRIARGRQLSYTQEQVQFKGH
                     AIECRVNAEDPFNNFIPSTGRITHSIIPTGPGVRVDTGVYPGFEITPYYDPMIAKLIV
                     WGETRAQAILRMRRALEEYRIVGVRTNIPFHQTLMDSHRFMGGQFDTRFVEERFSMEA
                     ASTLDEAQAEIAAILATLVSHKETERSAQIVRGNERDTSNWKWVGRWERMHR"
BASE COUNT          853 a          866 c          837 g          816 t
ORIGIN      
        1 acaaatgccg cccactctga ttgtctttgc aagttcattt tggcttatga ttggaatagc
       61 aaccttgctt agcatttggc aggaaaaacc ttggagtaaa aatctgctcc ttggggcagc
      121 agcaggatat accgtctggt attggagcga gcggctcatt tggcaaaatc cacgcccgaa
      181 cattccgttc acgattgtta taaattttgc atgtttgatc gttatctatt ttgcatcgaa
      241 atctttgtcg agagaggcat atgagcgaaa cactgaaaat ccagcaattg aatgacctga
      301 aagcgcaatc tcgtcttggc ggtggacaag cccgcattga cgctcaacat aaaaaaggac
      361 gtttaaccgc acgcgaacgc cttgaccttt tattggatcg cggctccttc cgcgaagtgg
      421 acccgttcgt tgtccaccgc acaacagact tcggactaga tgaacaaaaa atcatgagtg
      481 actctgtcgt cactggctgg ggaaccattg acgggcggct cgtgtatgta tattcacaag
      541 atttcaccgt ctttggtggc agccttggtg aagtgcatgc cgaaaaaatc tgcaagatca
      601 tggacatggc aatgaaaaac ggcgcccccg ttattggttt gaacgactct ggtggtgcac
      661 gtattcaaga aggcgttgtg gctttaggtg gctacgccga tattttcttg cgtaatacca
      721 tggcatctgg cgtaattcca cagatttctg ccattatggg accctgcgct ggcggtgccg
      781 tgtactcccc tgccctcaca gactttatct tcatgacacg caacacatcc tatatgttcg
      841 tgaccggacc cgatgtggtg aaagctgtaa ctcatgaaga agtgactcag gaagaattgg
      901 gtggtgcaag tgttcactca gaaaaatccg gcgtctgtca tgtcgcggcc gatagcgaag
      961 cagatacctt gtatctcatc cgcaagttga tgagctactt gccgcaaaac aacatggaag
     1021 actcacctgc ggcaccaaca tcggatgatc cgcttcgcat ggaagaagca ctcaattcac
     1081 ttgtaccaga tgatgccaat aaaccttacg acatcaaaga agtcatccgc cttattgtgg
     1141 acgatggtaa cttctttgaa atccatgaaa actacgccgc caatattgtg gttggtttcg
     1201 ctcgcctcgg cggacagtcc gttggcatcg ttgccaatca gcctgctgtc ctcgcaggcg
     1261 tgcttgatat tgatgccagt gaaaaaggcg cacgcttcgt tcgcttctgc gacgcattca
     1321 acatcccgat catcaccttt gaagatgtgc ccggcttcct gcccggaaca ttccaagaac
     1381 atcatggcat tatccgctcg ggagcaaaac tcctttatgc atattgcgaa gcaactgtac
     1441 cgaagttaac cgtcatcact cgcaaagcgt atggcggtgc gtattgcgtg atgtcttcca
     1501 agcacatccg cagtgacctt aaccttgcct ggcccacctc cgaaatcgct gtgatgggac
     1561 ccgatggtgc agtcaacatt atcttccgcc gcgagttgga agccgcagaa gaccccgtca
     1621 agcgcaaggc tgaattggtt gctgaatata aagagaaatt tgcgaacccg tatgttgcag
     1681 cctctcgtgg gtatatcgat gatgttatcg aacctaaaga aacccgtccg cgcttgatca
     1741 acgcacttgc catgttgagc aacaaacgcg attcaaaccc agccaagaaa cacggcaaca
     1801 ttccgctgta atcaggcgag gagagaatca tgtttaaaaa agtattggtt gcaaaccgtg
     1861 gcgagatcgc cgtccgcatc ttgcgcgctt gtcgcgaatt agggcttgaa accgttgccg
     1921 tattttccga agcagaccgc aacgcacttc atgtacgcta tgcggatgaa gcctatctac
     1981 tcggtcccgc tccttcacgt gaatcctatt tacgcacaga taagattctc gatgttgtaa
     2041 aacgctctgg ggcagatgct attcacccgg ggtacggctt ccttgcagag cgcgaagatt
     2101 tcgccgccaa atgcgcagac cttggcatca cctttattgg tcccaaacct tcagctattg
     2161 cggcgatggg cgacaaagcc gaagcacgcg ctacagtcat caaagctggc gtacccgttg
     2221 tgccaggcac cgaagacgtt ggcaacatga ccaacgatga cttactccgc attgctccta
     2281 cgattggctt cccgcttctt atcaaggcga cggcgggtgg cggcggaaaa ggcatgagag
     2341 aagtccgaag cttggaagaa atgccaactc ttcttgagtc cgcgaggcgt gaggcagaat
     2401 ctgcgtttgg cgatggcaat gtctatctcg agaaattgat cgagggcgcg cgtcacatcg
     2461 aattccaaat cattgctgat gaacatggca atgtcatcca cttgggcgaa cgtgaatgtt
     2521 ccattcaacg tcgccatcaa aaattggtag aagaagcgcc ctcacccttt ctcgatgaag
     2581 aattgcgcaa gcaaatgggc gatgtagctg tcaaagccgc gcaagctgtg gactatgtca
     2641 acgcagggac gattgaattc cttgttgata aagaccgcaa tttttacttc cttgaaatga
     2701 acacccgttt gcaggtggaa catcctgtga cagagttggt caccggtatc gacatcgtcg
     2761 ctgaacagtt gcgtatcgcc cgtggacgtc aactcagcta tacacaggaa caagtacagt
     2821 tcaagggaca tgcgattgag tgccgtgtca atgcagaaga cccgtttaac aatttcatcc
     2881 catctacggg tcgcattacc cacagcatca tccctactgg acccggcgtt cgtgtggaca
     2941 cgggtgttta ccccggtttt gaaattacgc cgtattacga tccaatgatt gccaaactga
     3001 ttgtttgggg cgagacacgt gctcaagcca ttttgcgcat gcgccgtgct ctggaagaat
     3061 atcgtatcgt gggtgttcgc actaatatcc ccttccacca aaccttgatg gactcgcatc
     3121 gtttcatggg tgggcaattc gacacccgct tcgtggaaga acgcttctcc atggaagcag
     3181 cttctacctt ggatgaagcg caggcagaga ttgcagccat cctcgctacg ctcgtatctc
     3241 acaaagagac ggaacgctct gctcagatcg tccgtggtaa cgagcgcgac accagcaact
     3301 ggaagtgggt cggtcgctgg gaacgcatgc accgctaatt agaagaggct atggcatgaa
     3361 atatataact ac
//
LOCUS       DAOQJE010000331         3364 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_18254_length_3364_cov_6.968571, whole genome shotgun sequence.
ACCESSION   DAOQJE010000331 DAOQJE010000000
VERSION     DAOQJE010000331.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3364)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3364)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3364
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18254_length_3364_cov_6.968571"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..399)
                     /locus_tag="PLT08_09515"
     CDS             complement(<1..399)
                     /locus_tag="PLT08_09515"
                     /inference="COORDINATES: protein motif:HMM:NF022072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HNE04744.1"
                     /translation="MFNVSFRVQTIIKATPEAIFPLLADLTRHGEWSANPVQIEAVTK
                     GTPQVGSVYRSAAQVNNLHFSAELTVNEYQPPLSFAFTGKDETGTFKHQFRLSPEPNG
                     TRVERRVSFELSFRQWLMYCLLLFPVRLPAA"
     gene            1079..2410
                     /locus_tag="PLT08_09520"
     CDS             1079..2410
                     /locus_tag="PLT08_09520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE04745.1"
                     /translation="MPYIIFPLPWNIYKRNYSLSRVLPILMCLPVLAIYAPAYSNTIF
                     PFINNLFDVSYFLLFVPLGWLMLLLLISPAMQRRARPEAAFTRFANPLSTKGITPTSV
                     SFFIDHASEDGKYAEDLRAVLEKHGHVYARPDLPAEAIFVLLSSYKKETKYDPDQQSV
                     FPILLQAVTDIEVKLQRIQWIDFRKGIKNAKALARLLPEPQKLLKALASPPTGAQEIF
                     PTAVSVLQYFYLFVGLLGGGSILLSILTLVSEIVSRTINLDIFFEIVFLTIQGILLAG
                     TIVLSVRGLRFRRGGISSIYPLIVLLLFQASIYFSNFLSSVRYYSLSSDASISFLNIS
                     FSGIAFLGGLGISAVFIALGWKDLYRWFPRRQSEITNFIERTFLLYTPKDTKALFLHA
                     LFHIVLLFAFVAPWFGAPFGPIVMLSIFFVCLSVNGLILVPIYWFAKRYSR"
     gene            complement(2439..2816)
                     /locus_tag="PLT08_09525"
     CDS             complement(2439..2816)
                     /locus_tag="PLT08_09525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE04746.1"
                     /translation="MTHTVSYNLELSVIEVYVYGDFRLSEGMEILQKTIAILKQSNCR
                     HVVQDIRKANLKFSLVELFMVPKSIQDALTREGLSFQAHRWSVVANDYSSNLKFVENV
                     VNNRGYTMKVFTDFDKALKWLQG"
     gene            3052..>3364
                     /locus_tag="PLT08_09530"
     CDS             3052..>3364
                     /locus_tag="PLT08_09530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE04747.1"
                     /translation="MSKTFNPPFYIRSAYAQTFLASNKFGTQWNNPILKNSKEVILNP
                     VGQVRLQGFYSPQPNAKGLVILLHGWEGSVNSAYILRTAKFLYQNGFSVFRLNFRDHG
                     ET"
BASE COUNT          909 a          760 c          692 g         1003 t
ORIGIN      
        1 ggcggcgggc aaacgcacgg ggaacaacag caggcaatac atcagccact gccgaaacga
       61 tagctcgaag gacacgcgac gttccacgcg agtgccgttc ggctcagggc ttaaccggaa
      121 ttggtgctta aaggtcccgg tctcgtcctt gccggtaaag gcaaaagaca aaggcggctg
      181 atattcgttc acggtcagct cggcagaaaa atgcaggttg ttgacctgcg ccgccgaacg
      241 atacacgctc ccaacctgag gcgtgccctt cgtcaccgcc tcgatctgca ccgggttggc
      301 agaccattct ccatgccggg tcaagtctgc gagcagagga aagatcgcct cgggtgtcgc
      361 tttaatgatg gtttgaacac gaaaagaaac attaaacatt agtatacaaa actcctatac
      421 gcttgctttg aaagcagaaa ttccgctgat tgtacaggaa aaatcccgca ggcatacgat
      481 taaaaatata tacccaagca agaatagctc ctttagaatg tatactatac aaaactattg
      541 gtgattctta ttatatagag gcagcaagga ttactttatg aacaagaatt tgatgatcag
      601 ttattcacgc caacaaactc cttttgtgga tcgcttttat gatgacctgc aaagatcagg
      661 gtataccctc tggctggatt atcacagctt agtccctgcc caaccttggt atcaacagat
      721 tgaaaaaggt gtcttaggag cagatgttgt gatccttgtt gtttcacaat cttcgatttt
      781 ttctaaaaat gtcgaaccgg aatggcggct ggcattggaa cataaaaaac ggatcattct
      841 tgcggttttt gaggcaacac cattgccgcc tgaattgagc aatttcgaat gggtcgattt
      901 tcggacaaat tatcgaagag ggattaaaga acttaaaggc ttgctaaatt acccagaaca
      961 aacagtcaca ccgcctgtac ctcagaaagg ttttcgagct ccgcttacct tttgggttgc
     1021 attgcttatc agcctattcc ttgtacttgg cacaatccca ttggtttgga ccatcattgt
     1081 gccttatatt atctttcctt taccgtggaa tatttacaaa aggaattatt cgctttccag
     1141 ggtcttgcca atattgatgt gccttcctgt tctggcaata tatgcgccag catattcaaa
     1201 tacaattttt ccttttatca ataatctttt tgatgtttca tattttcttt tgtttgtccc
     1261 actaggctgg cttatgctct tgcttttgat ctcacctgcc atgcaacgac gggcaagacc
     1321 tgaggcagcg tttactcgat ttgcaaaccc actctccacg aaagggataa ctccaacgtc
     1381 tgtttctttt ttcattgatc atgcctcaga agacgggaaa tatgcggagg accttcgggc
     1441 agttctggaa aaacatgggc atgtctatgc tagacccgat ctgcccgccg aggcgatttt
     1501 tgtcttgtta tccagttata aaaaagaaac aaaatatgac ccggatcaac aaagtgtgtt
     1561 tccaattttg ttgcaggcag taacagacat tgaagtgaaa ctacaaagaa ttcaatggat
     1621 cgattttaga aagggaataa aaaatgcaaa ggctctggca agactattgc cagagcctca
     1681 aaagctatta aaggctttgg cttccccgcc gacgggggca caggagattt ttcccactgc
     1741 tgtgagtgtg cttcaatatt tttacctctt tgtcggtttg ttgggcggag gatcaattct
     1801 tctttctatt cttacattgg ttagtgagat tgtcagccgt acaataaatt tagatatctt
     1861 ttttgagatt gtttttttga cgatacaagg gatattgttg gcaggcacta tcgtactttc
     1921 tgtacgagga ttgcgcttta gacgtggagg aatttcttcc atatatccat tgattgttct
     1981 tttgttgttt caagcaagta tttatttttc aaattttctt tcttctgtaa gatattatag
     2041 tctttcttct gatgcatcta tatcctttct taatatttca ttttctggaa ttgcattttt
     2101 aggcgggcta gggatttctg ctgtatttat agctttgggt tggaaggatc tatatcgctg
     2161 gtttccccgc cgccagtcag aaattactaa ttttatagaa cgtacatttt tgctttatac
     2221 accaaaggat acaaaggcac ttttcttgca tgcgttgttt cacattgttt tattgtttgc
     2281 ctttgtagca ccatggtttg gtgccccatt tggacccatc gtgatgcttt ctatattctt
     2341 tgtttgtcta tctgtaaacg gactgatatt agtgccaatt tattggtttg ccaaacgata
     2401 ttcaaggtaa accaaggagc actctcaata tacataattt agccctgcag ccacttcagg
     2461 gctttatcaa aatccgtaaa taccttcatg gtatatccgc ggttgttcac tacattctcc
     2521 acgaacttca agttcgaact gtagtcattg gcaaccaccg accacctgtg cgcctgaaaa
     2581 gaaagccctt ctctcgtcag cgcatcttga atagactttg gcaccataaa taactctacg
     2641 agcgagaact tcaaatttgc cttgcgtata tcttgcacaa catggcggca atttgattgt
     2701 ttcaatatgg ctatggtttt ttgaagaatt tccattcctt cactaagtct aaaatctccg
     2761 tacacataca cctcaatcac agataattcc aaattgtatg aaaccgtatg cgtcattgac
     2821 gactccaaag gcaagaagag tagttggtgc taagcagaga ccacaagctg caagatggtc
     2881 caaaacgaaa ccaatgataa ggtttctacc atttaatagt agactggcga aagagcaaat
     2941 acaggtttcc cctgattctc ttgccctacc tcaccaactt aacgagaata aaagtagacc
     3001 taaatagtct gattgacaat cctgttctga ggcagataca atccaaactg tatgagtaaa
     3061 accttcaacc ctccctttta tatccgcagt gcctacgctc aaaccttcct tgccagcaac
     3121 aaatttggca cgcaatggaa caatcccatt ctcaagaact cgaaagaggt catcctcaac
     3181 ccggtggggc aggtgcgctt gcagggcttt tactcgccac aaccgaatgc caaaggtcta
     3241 gtcattcttc tgcacggctg ggagggcagt gtcaactcgg cgtatatttt acgcactgcc
     3301 aagtttctct accaaaacgg cttctcggtc ttccggctca acttccgcga ccacggcgag
     3361 acac
//
LOCUS       DAOQJE010000332         3364 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_18256_length_3364_cov_5.954367, whole genome shotgun sequence.
ACCESSION   DAOQJE010000332 DAOQJE010000000
VERSION     DAOQJE010000332.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3364)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3364)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3364
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18256_length_3364_cov_5.954367"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            191..1114
                     /locus_tag="PLT08_09535"
     CDS             191..1114
                     /locus_tag="PLT08_09535"
                     /inference="COORDINATES: protein motif:HMM:NF024950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HNE04748.1"
                     /translation="MSLPNQQAILVTGASSGIGKHLTLRLAERGHIVYATVRKEKDMA
                     ALSEIPNVTSLLMDVRNVSQIQSAVEVVSNAGHGLYGLVNNAGVGELGLLTTWTDEEM
                     LDIFNVNVFGPHRMSNAFMNLLLESRGRIVNIGSQGGMLASKYYGPYVMTKHALEAYT
                     AVLSEELKSYGVQVSIVQPGGIITEIGEKSQAGTLAHFQRAQPPFKEEADLILASFSE
                     EAEPNPDEPESATNRKPSSPEIVWEAVQDALFSEKPKLRYLVGTHWEGNRVLNALMSK
                     LLDENDNPQHNYSRDELIALLDQHLTERNAS"
     gene            1166..1900
                     /locus_tag="PLT08_09540"
     CDS             1166..1900
                     /locus_tag="PLT08_09540"
                     /inference="COORDINATES: protein motif:HMM:NF019349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HNE04749.1"
                     /translation="MAAKELSHKPLKEEIYDALHRQIIAGRYGPGDWLRQEDIASQMG
                     VSMTPVREALDLLVAKGIAERVPYRGVRVREMSPEDVVEAYGMRLFLEAMVARETALN
                     ITPEQVQKLENIMAEMDRHVELNEMPQERQLSREFHSAIAEASGNKLLVELYSIVSNA
                     FPDWLLYEALYRQPELVSSSVSQTHDEHAAILNAFKKHDPDLAVKVSLEHVMESGRWL
                     ENYRNIPAKLLREKEKQVSHLIKKSK"
     gene            1918..2577
                     /locus_tag="PLT08_09545"
     CDS             1918..2577
                     /locus_tag="PLT08_09545"
                     /inference="COORDINATES: protein motif:HMM:NF014375.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="corrinoid protein"
                     /protein_id="HNE04750.1"
                     /translation="MSEEIEKLFKDMAQSIIDGDSDLSVELAEKSIAMNIPPLDAITK
                     GFVIGVNYIGDQFGAGEAFLPELVMAGEAMKAAVATLEPELLKLGEARETMGKVILAT
                     VEGDIHEIGKTLVGTMLSASGFEVTDLGVDQPADKIIGKALEIDAQIIGLSALLTTTM
                     VRQREIIEELDKENLRPRIKVMVGGAPITRDWATKIKADGTSEDAVGAVALAKQLVGK
                     A"
     gene            2624..>3364
                     /locus_tag="PLT08_09550"
     CDS             2624..>3364
                     /locus_tag="PLT08_09550"
                     /inference="COORDINATES: protein motif:HMM:NF018012.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0015948 - methanogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trimethylamine methyltransferase family protein"
                     /protein_id="HNE04751.1"
                     /translation="MSHKKIKTISDPKLKLDILSKEDVQKIHEATLHIIENVGIRFPS
                     KRALRIWEEAGATVDHEKKIVKVKPYLIEDALKQAPPAYTLGARDPQQNLSLDGNHVH
                     LGTDGCGVEIIDIHTGMKRTTELQDVCDIARIADATEEVAFHWVPVSAQDAPVEARGL
                     HELKAVWENSTKHVQTESIYNVEEAKASMEMAILLAGSKEDLRKRPVLSLMQCTAPPL
                     GHDGGSLDAALIAAEHGVPTGFMTMTACM"
BASE COUNT          991 a          753 c          841 g          779 t
ORIGIN      
        1 acgacaattt gatttttgaa tacactgcaa cagatcaaac ttctcaagaa ataacaatca
       61 actcagactt gactgttaca gcaaaatgat agtcttaaat caaaatccgg agatgacatc
      121 tccggatttt gatttattat atagctactg aaaactttag agtatatcga gaataaaaag
      181 gtaaaagaac atgtctcttc caaatcaaca agcgatttta gttactggcg ccagtagcgg
      241 cattggaaaa cacctcacac tacgcctcgc cgagcgtgga catatcgtct atgccactgt
      301 gcgaaaagaa aaagatatgg cggcgttatc tgaaattcca aatgtcacgt cattgttaat
      361 ggatgttcgc aacgtgagtc agattcaatc tgcggtagag gttgtttcaa acgcagggca
      421 tggactctat ggcttagtca ataacgcggg tgtaggagag cttgggttgc taactacatg
      481 gacagatgaa gagatgctgg atatcttcaa cgtcaatgtg tttggtccgc atcggatgag
      541 taatgcattt atgaatctat tacttgaatc tagaggacgc attgttaaca ttggatctca
      601 aggtggtatg ttggcaagta agtattacgg tccatatgtg atgacgaaac acgccttgga
      661 agcctataca gctgtattga gcgaagaatt aaaatcgtat ggtgtgcagg ttagcattgt
      721 tcagcccggc gggatcatca cggagattgg ggaaaaatct caggcgggaa ctctggctca
      781 ttttcagcgt gcccagcctc cgtttaagga agaggcggat cttatccttg cgagtttttc
      841 agaggaagcg gaaccgaatc cagacgagcc ggaatccgct acgaatcgca aaccatcttc
      901 tcctgaaatt gtttgggagg ctgtacaaga tgccttgttt agcgaaaaac caaaactgcg
      961 ctatttagtg ggcacacatt gggaaggaaa tcgcgtgctt aacgctttga tgtcaaaact
     1021 attggatgaa aatgacaacc cacagcataa ctattcacgg gatgaattga ttgcattgtt
     1081 agatcaacat ctgacagagc gaaacgcttc ttagttaggc acttccgaaa accgtctaga
     1141 ttatatataa tgtacaatta tggtaatggc agcaaaagag ctttcacata aacccttgaa
     1201 agaggaaatt tacgatgcct tgcaccggca gatcattgcg gggcggtacg gtcctggcga
     1261 ctggctgagg caggaagata tcgccagcca gatgggcgtc agcatgaccc ccgtccgcga
     1321 ggcgcttgac cttttggtcg caaaaggcat cgcggagcgt gtcccctatc gcggcgtgcg
     1381 tgtgcgggaa atgtcgccag aagatgtggt ggaggcgtat ggcatgcgcc tctttcttga
     1441 ggcgatggtg gctcgggaaa ccgcgcttaa tattacgcct gagcaggtgc aaaagcttga
     1501 aaacatcatg gcagagatgg atcgccatgt agagttgaac gaaatgcctc aagaacgcca
     1561 actgagccgt gaatttcata gcgcaattgc agaggcatca ggaaataaat tattagtaga
     1621 gttgtattcc atcgtctcaa acgcatttcc agattggttg ctatatgaag cgctttatcg
     1681 tcaacctgaa ttagtttcaa gcagtgtctc acagactcac gatgaacatg cagccatttt
     1741 gaatgcgttc aaaaaacatg accctgattt agccgtaaaa gtctctctgg aacatgtcat
     1801 ggaatctggc agatggttgg aaaattatcg taacatcccg gcaaagttat taagagaaaa
     1861 agaaaaacaa gtttcgcact tgatcaaaaa atccaagtaa attcatcagg aggcagtatg
     1921 tcagaagaaa tcgagaaatt attcaaggat atggcacaaa gcatcattga cggagactct
     1981 gatctttcag tggaactcgc cgaaaaatct attgcaatga atattccccc gcttgatgcc
     2041 atcacaaaag gatttgtgat cggcgtcaat tacatcggtg accagttcgg cgcaggcgaa
     2101 gcctttttgc cagaattggt tatggcaggt gaagcgatga aagccgctgt agcaaccctc
     2161 gagcccgaat tgctcaaact cggtgaagcc cgcgagacta tgggcaaggt cattctcgcc
     2221 accgtagaag gtgacattca cgaaattggt aagaccctgg ttggcaccat gctcagcgcc
     2281 tctggcttcg aagtgaccga cttgggcgta gaccagcccg cagataagat cattggcaag
     2341 gcgttggaaa tcgatgcaca aattatcggt ttgagcgcat tgctcaccac caccatggtg
     2401 cgtcagcgcg agatcatcga agagctcgat aaggaaaatc tgcgcccgcg tatcaaggtc
     2461 atggtcggcg gggcaccgat cacccgcgac tgggcaacca aaatcaaagc agatggcact
     2521 tccgaagatg cagtcggcgc agtggcattg gcgaaacaat tagtcggaaa agcataatca
     2581 aacaatcaga gagagaagag agacatagcc tcgggagggc tctatgtctc acaaaaaaat
     2641 caagaccatt agtgatccaa aactcaaatt ggacatacta agcaaagaag atgtgcaaaa
     2701 aattcacgaa gcgacgttgc acatcattga aaatgtcggc atacgttttc cttcaaaacg
     2761 agcattgcgc atctgggaag aagccggtgc aacagttgac catgagaaga aaatcgtcaa
     2821 ggtcaaaccg tatctgatcg aagatgcgct caaacaggct ccacctgctt atacacttgg
     2881 agcgcgtgac ccacaacaaa atctctcact cgatggcaac catgttcatc ttggcacaga
     2941 cggttgtggt gtagagatca tcgacattca caccggcatg aagcgcacta ccgagttaca
     3001 agatgtatgc gacattgccc gtattgcaga tgccacagaa gaagtggcat ttcactgggt
     3061 gcctgtttcg gcacaagatg ctcccgttga agcacgcgga ttgcacgaac tcaaagcggt
     3121 gtgggaaaac tccaccaagc acgtgcaaac cgagtccatt tacaacgtag aagaagccaa
     3181 agcctcgatg gaaatggcga ttctgctcgc aggcagcaaa gaggatttgc gcaagcgtcc
     3241 cgtcctctcg ttgatgcagt gtactgcgcc tcctctcggg cacgacggcg ggtcgctcga
     3301 cgcagccttg atcgccgctg aacacggagt gccgaccggc ttcatgacca tgacagcctg
     3361 tatg
//
LOCUS       DAOQJE010000333         3361 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_18283_length_3361_cov_6.238657, whole genome shotgun sequence.
ACCESSION   DAOQJE010000333 DAOQJE010000000
VERSION     DAOQJE010000333.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3361)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3361)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3361
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18283_length_3361_cov_6.238657"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(1..677)
                     /gene="tgt"
                     /locus_tag="PLT08_09555"
                     /pseudo
     CDS             complement(1..677)
                     /gene="tgt"
                     /locus_tag="PLT08_09555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011184131.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008479 - queuine tRNA-ribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
     gene            864..1409
                     /locus_tag="PLT08_09560"
     CDS             864..1409
                     /locus_tag="PLT08_09560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE04752.1"
                     /translation="MTKPRLTHSLFLILLLTACAAPATAAPTQTSTPKAPEATATIDN
                     STPTLTVTPDAKLATVEGTMTWLPSGTDEILPVPDVTMQLDRHDGEYLKYKVRTEANG
                     HYVFANIEPGTYGVGMYLNLRVGERQCGAPEFTSTLDLGWEHYSTWSKVDVWYDVIFS
                     DADITIKPGEAITIDYQLKCP"
     gene            1426..>1676
                     /locus_tag="PLT08_09565"
     CDS             1426..>1676
                     /locus_tag="PLT08_09565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE04753.1"
                     /translation="MNIKKVLPVVLIAGFLLIAACMSSPTPTELPIPTPMTVELTQPP
                     HLDPKYFLTATLVPFVTLAPTSTFEGTPAIVNIALNMGAG"
     assembly_gap    1677..1776
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            1911..3338
                     /locus_tag="PLT08_09570"
     CDS             1911..3338
                     /locus_tag="PLT08_09570"
                     /inference="COORDINATES: protein motif:HMM:NF012382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HNE04754.1"
                     /translation="MKTLLIPLDTNATKALYLQVADYITEAARSGQLKPGTQLPPSRT
                     LAEQNNVHRSTIINAYQELKARGVLASKQGSGTYIEKGLSENTVSIQKFKPESLPTDE
                     LIAEIRRMHWSEGMISLGYGPPSDELMPVEDFDRSRQRVLRRDGKKTANYEHPQGYYP
                     LRQAICADLVKQGIQADPDEILITNGVLEGVALVSRAMAAPGDAVLTELPQYFGNWTN
                     FSYLGLNVRGFDLLESGPDFASLEAQLQDENDRPRFVFVTADHQNPLGTCWTMPARHE
                     FLKMMNKYDVPVVEDATYRDLTYDGMPHTPLRALDPDVIYVGTFSHSLMPGLRIGFVL
                     CRGRLREHIERLKAISSGPVETLNQRALADFLATGEYERHLDRIIPVYRARRDALLNA
                     MQTYFPPELRWTKPTGGFFVWIWLPDHISAIDLFYRSVKKGISIAPANIFYPGPCPQN
                     AFRLAFSHYPEDVLTRAVMEIGKLL"
BASE COUNT          825 a          901 c          772 g          763 t
ORIGIN      
        1 ccaacgccca ttagatagcg cggcttattc tcaggcaaca acggtgtgac cacatccagc
       61 gcatcatgca tctcttgctt ggtttcaccg acagataaac caccaatcgc aatgccgggc
      121 gtctctaacg aggcgatgaa tttggcagac tccgctcgca gcgtaggatc cactccacct
      181 tggacgatcc cgaacaaggc ttggtctgag cgcgtctttg ctttcagcga acgctccgcc
      241 caacgatgag tccgctccat ggctttcttg atatatgcat ggtcgttcgg gtcagaacat
      301 tcatcaaatg ccatgatgat gtcggcgccg agattctctt gaatcgcaat cgacttctct
      361 ggcgtaaaac gatgcgtaga cccatcgata tgactcttga aggtcacgcc atcttcatcc
      421 actttgcgag tttgcgaaag agaaaaaacc tgaaagccac ccgaatcagt aagcatgggg
      481 ttatgccact gcatgaattt atgcaacccg cccatatcgc gcaccaattc atcgccaggg
      541 cgcagataca aatgataggt attgctcaag acgagtgaag cgttaatgct cttgagatgt
      601 tcaggcgtca gtgttttgac agtggcttgt gtacccactg gtgcaaacac aggagtaatg
      661 agatcgccgt gcggcgtgga gaaaaccccg gtgcgggcgc ggttattttt agctgtaatg
      721 ctgaaatcaa acatggggca aattatacag gatgaaagtg tatcaccgaa ttggtatgca
      781 aactgcccct caattggctc tttttcaggc atagaccgtt tcatataatt gcaataatag
      841 acttattctt agagagagaa atcatgacaa aaccccgcct cacccatagc ctatttttga
      901 tcctgttgct gacagcttgt gccgcgcccg caactgccgc tcccacacaa acctccaccc
      961 ctaaagcacc cgaagcaacc gccaccatcg acaactccac acctacattg acagtcaccc
     1021 ccgatgcgaa gcttgctact gtagaaggga ccatgacttg gctgccctct ggaacggatg
     1081 agattttgcc tgtgcccgat gtgactatgc aactggatcg ccatgatggt gaatatctca
     1141 agtacaaagt tcgcaccgaa gcgaacggac attatgtctt tgccaatatc gagccaggaa
     1201 cttatggcgt cggcatgtat ttgaatttgc gggtcggtga acgccaatgt ggagcccccg
     1261 aattcacctc caccttagat ttggggtggg aacactactc aacatggagc aaagtcgatg
     1321 tctggtacga cgtcattttc tcagatgcag atatcaccat caaacccggc gaagccatca
     1381 ctatagacta tcaattgaaa tgcccgtaga gttcaggaga gactgatgaa tatcaagaag
     1441 gttttacccg ttgtattgat tgctgggttt ctgctcattg cagcgtgcat gtctagccca
     1501 acgccaactg agttacccat tcccactcca atgaccgtgg aattaactca gccgccccac
     1561 ctcgacccca aatactttct caccgccact cttgttccgt ttgtaaccct cgccccaacc
     1621 tccaccttcg aagggacgcc tgctattgtc aacattgcct tgaacatggg cgcaggnnnn
     1681 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1741 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnccgt tgatcgccat gaaagtggag
     1801 ccgggtcagc gctacaacat tctcattgca gaccgcaaag ctctgctacc tgaaatcaac
     1861 gaacgcggat tgccagtgac cgttccatac gtactggtag aatccaaccc gtgaaaacat
     1921 tgctcatccc tttagatacg aacgccacca aagcgcttta cctgcaagtt gcagattaca
     1981 tcaccgaagc cgcccgcagc ggacagttaa agcccggcac acagttaccg ccttcgcgca
     2041 ccttggctga gcagaataac gtccaccgct caactattat caatgcctat caggaactca
     2101 aagcgcgtgg tgtgctggca tccaaacaag gaagcgggac ttacatcgaa aagggattga
     2161 gcgagaacac cgtgtcgatt caaaagttca agccagaaag cctgcccacc gacgaactga
     2221 tcgcagaaat tcgccgtatg cattggtctg aaggcatgat ctcacttggg tatggtccgc
     2281 cgtcagatga gctgatgccc gtggaggact tcgaccgttc acgtcagcgg gtgttgaggc
     2341 gcgatggaaa aaagacagcc aactacgaac atccgcaagg gtattatcca ctgcgccaag
     2401 ccatctgcgc agacctcgtc aagcagggta ttcaagcaga cccagatgaa atcctcatca
     2461 caaatggagt cttggaaggc gttgcacttg tgagccgtgc catggcggca ccgggtgatg
     2521 ccgtgttaac tgaactgcca caatattttg gcaattggac aaatttttcg taccttggtt
     2581 tgaatgtgcg cggcttcgac ctgctcgaaa gcggacccga ttttgcgtcg cttgaggcgc
     2641 agcttcaaga tgaaaatgac agaccaagat tcgtcttcgt gacagccgat catcaaaatc
     2701 cgcttggcac ttgttggacg atgcccgccc gccacgaatt tctcaagatg atgaacaaat
     2761 acgatgtgcc tgtcgtagag gatgcgacct atcgcgactt gacttacgat ggcatgcccc
     2821 acacaccgct tcgggctctg gacccagacg tgatctatgt cgggacgttt tctcattcgc
     2881 tgatgcccgg tctccgtatc ggattcgtgc tttgccgtgg gcgtctgcgc gaacacattg
     2941 aacgcctcaa agccatctcc agcggacccg tcgaaacgct taaccaacgc gcgctggcag
     3001 attttcttgc tacgggtgaa tatgaacgtc atcttgatcg cattatcccc gtctaccgcg
     3061 cacgacgcga tgcgcttctg aacgcgatgc aaacctactt cccgccagaa ctccgctgga
     3121 cgaaacctac cggcggcttc ttcgtgtgga tctggttacc tgaccatatt tctgcaattg
     3181 acttgttcta tcgttccgta aaaaaaggaa tcagcatcgc ccccgcgaac atcttctacc
     3241 ccggtccctg cccgcaaaat gcatttcgtt tggcattctc tcattaccct gaagatgtgt
     3301 tgacaagggc agtaatggag attgggaagt tgttgtaatt aaaacaaaag acctcggagg
     3361 t
//
LOCUS       DAOQJE010000334         3359 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_18294_length_3359_cov_5.926453, whole genome shotgun sequence.
ACCESSION   DAOQJE010000334 DAOQJE010000000
VERSION     DAOQJE010000334.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3359)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3359)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3359
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18294_length_3359_cov_5.926453"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            276..737
                     /locus_tag="PLT08_09575"
     CDS             276..737
                     /locus_tag="PLT08_09575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE04755.1"
                     /translation="MNTYSLRGISRIHSKGAGWLVRLYRNAKVITKLFSDGVYGDSDK
                     ALQAAQAYHRQAQIKFPPIEKPPFRETPLRNNTSGYNGICETFSRTRKGEKISCWNVS
                     WYNPPNKLNSKKFYFHDPQERKQALKEALQFRKEREAEILKQSRRRRKITA"
     gene            780..962
                     /locus_tag="PLT08_09580"
     CDS             780..962
                     /locus_tag="PLT08_09580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE04756.1"
                     /translation="MAFVVFSGKVLVSRQATQQSVHLTLGILRKSQAVSHALAFFWLD
                     GFAVPTPAQVTQTVGR"
     gene            1071..1520
                     /locus_tag="PLT08_09585"
     CDS             1071..1520
                     /locus_tag="PLT08_09585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE04757.1"
                     /translation="MFGEGRWWFCQACDTLVISYANQDLDVHSILFALGDQAMNSVWQ
                     VSGVDCFGENAEELWLIDDNKQSVRGNDLLRITTGITQTIDGDFHAFDNCSTSHWFYM
                     RAWDGSGFYVETNDHKVKERLDARFRNVEDVAETQPPYEGLFIPRGD"
     gene            1713..2444
                     /locus_tag="PLT08_09590"
     CDS             1713..2444
                     /locus_tag="PLT08_09590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2726 domain-containing protein"
                     /protein_id="HNE04758.1"
                     /translation="MATKKKEKQGCLSVIFPFLGRKTTKTVFTASSYKAPDEPTPIVE
                     ANIPEVLPYRVRDDFLSPAEFSFYKILSSLGGTRLIIQSKVRLADVFFVARPNENMTY
                     FSRIAQKHLDFLVCDSVTMKPLLGIELDDSSHKRDDRQERDDFVDRVFEVAGLPLLRL
                     PVQREYNTREVAAKIAPLLKDKISPSTTPPQPEINKQENGIPLCPKCGIPMVLRTVSQ
                     GEHKGKQFYGCKNYPRCREMKPFTA"
     gene            2627..3313
                     /locus_tag="PLT08_09595"
     CDS             2627..3313
                     /locus_tag="PLT08_09595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE04759.1"
                     /translation="MTLPPFDLTLLINWVTSGFIGLIFGLISASVTYRYEKKRDEKAW
                     EREKEKIQLQFQHDRDMFEAEVNQKIKELELQFQQRETEKIRQTLLAGLGNPSEVISR
                     VERTNQIIKQEPKYVSYNLYIRPSFLNYLSTMENIFWLVLGVMVTVTVNSIISRHIPS
                     PVWLWLILFGIFLFAEVILAFFIQHYKKVKATDDLMKEFYYEQSLELLKMKQKIQEQF
                     RTASEKQNTG"
BASE COUNT         1025 a          708 c          744 g          882 t
ORIGIN      
        1 aaggaagata accgaaggaa atctggaaaa taaagttgtg ttaaagtcaa acgccaccta
       61 acaaagcgtg cacttgacgg gtgggattct gcgccatttt cgggcttttt tctacacccg
      121 aacagaatcc tgctctcgaa gttttctctc atcccgccca cccgcaagta acgccaaccg
      181 ttgggttgcc cccgtgcaaa acataaagag ccttgaataa ttgtcagata taattccttt
      241 catctctcta ttaatgaaag gaatttgaca gacaaatgaa tacttactct ttgaggggta
      301 tcagcaggat acactcaaag ggggctggct ggttagtcag gttgtatcga aatgctaaag
      361 tgattaccaa actctttagc gatggcgttt atggtgactc cgataaagca ttacaagcag
      421 cacaggctta tcatcggcag gcgcaaataa aattcccgcc catagaaaaa ccgccatttc
      481 gggaaacgcc acttagaaat aatacttctg gctacaatgg catttgtgag acgtttagcc
      541 gtactagaaa aggcgagaaa atatcgtgct ggaatgtcag ttggtataac ccgccaaaca
      601 agttgaatag taaaaaattc tattttcatg acccgcaaga acgtaagcaa gccctgaaag
      661 aagcacttca atttcgtaaa gaacgagaag ccgagatttt gaagcaatct cgcagaaggc
      721 gaaaaatcac ggcataagtt atttcaagaa tcacgcctga aaaaattgca ttgggtcttt
      781 tggcgtttgt agttttttca ggcaaggttc tggtaagcag gcaggcaacc caacaaagcg
      841 tgcacctgac gctggggatt ctgcgcaaat ctcaagcagt ttcacacgcc ttagcatttt
      901 tctggctgga cggcttcgcc gtccccaccc cagcgcaggt aacgcaaacc gttgggcgct
      961 aaacagatca tagcgagaat tgttttctta tgaaaagata tcagttttgg tgtccgaaat
     1021 gcagaaaagg attctacgaa catgaagttg ggaggagaga ggttgatacc atgtttgggg
     1081 aaggacgttg gtggttttgc caagcgtgcg atactttggt catttcatat gccaaccaag
     1141 acttggatgt acacagcata ctctttgcac tgggtgatca agcaatgaat tctgtatggc
     1201 aggtaagtgg tgtagattgt ttcggagaaa acgcagaaga attatggttg atagatgata
     1261 acaagcagtc tgtccgagga aatgatttgc ttcgtatcac aacaggtatc acccaaacca
     1321 ttgatggtga ttttcacgca tttgacaact gctcaacatc tcactggttt tatatgcgtg
     1381 cttgggatgg tagtggattt tatgttgaga caaatgacca taaagtcaag gaacgtttgg
     1441 atgcccgatt tcgaaacgtt gaggatgttg cagaaacaca gcctccctat gaggggctat
     1501 ttattccacg tggcgattaa aagccaaaaa gcgcccaaca aagcgtgcac ctgacgctgg
     1561 ggagtctgcg cacatcccaa gcagttttct acgccttatc atttttctgg ttggacggct
     1621 ccgccgtccc cgccccagcg caggtaacgc atgccgttgg cacgcccctt gcacaacaaa
     1681 gtaaaatttt cggtggagaa aaaatatagc acatggctac aaagaagaaa gaaaaacaag
     1741 gttgtttaag cgtcatattt cctttcttgg ggcgcaaaac gactaaaact gtttttactg
     1801 caagttcata taaagcgccc gatgaaccta ctccaatagt tgaagcaaat attcctgaag
     1861 tgcttcccta tcgtgtgcgt gatgattttc tttctcccgc tgaattctcg ttttataaaa
     1921 tcctttcttc tctcggtggc acacggctaa taattcaatc aaaagtaaga ttggctgacg
     1981 ttttctttgt ggctcgccca aacgaaaaca tgacgtattt cagtagaatc gctcaaaagc
     2041 acttggattt tctagtgtgc gattctgtta cgatgaagcc gttactcggc atcgaactgg
     2101 atgattccag tcataagcga gatgacagac aagaacgtga cgattttgtt gatagagtct
     2161 ttgaagttgc tggtttgccg ttattgcgtt tgcccgttca gcgagagtac aatacaagag
     2221 aagttgcggc taaaattgcg ccgcttttga aagataagat tagtccttca actacgccgc
     2281 cacagccaga aataaataag caagagaatg gaataccact ttgtccaaaa tgcggtattc
     2341 caatggtttt acgaacagta tcgcaaggcg aacacaaagg aaagcagttt tacggttgca
     2401 aaaattaccc tcgttgtcga gaaatgaaac cctttacagc ataggtgagt cttgcaaatc
     2461 aaaagcgtgc caacaaagcg tgcacctgac gctggggatt ctgcggcatt ttcaggcatt
     2521 ttcctacgcc ttatcatttc tctggctgga cggcttcgcc gtccccgccc cagcgcaggt
     2581 aacgcaaacc gttagcccgt tgaccctaaa aaggtaaaca taatacatga cacttccacc
     2641 ttttgacctt acgctcttga taaactgggt aacatctggt tttataggct taattttcgg
     2701 attgattagt gcatcagtta catatcgtta cgaaaagaaa agggatgaaa aagcatggga
     2761 aagggaaaaa gaaaagatcc aactgcagtt tcaacatgat agagatatgt ttgaagcaga
     2821 agtaaatcag aagataaaag aattggaact tcaattccag caacgtgaaa ctgaaaaaat
     2881 ccgacaaact ttgttagcag ggctcgggaa cccatctgaa gttattagca gagtcgagcg
     2941 aaccaatcag attatcaaac aagaacccaa atatgtctca tataaccttt acattcgccc
     3001 aagtttcttg aactatctat ctacaatgga aaatattttt tggttagttc ttggcgttat
     3061 ggtaacagta actgttaatt cgataatttc acggcacatc ccatcaccag tatggctatg
     3121 gctaattcta ttcggcattt tcctatttgc cgaagtaatc ttagcgtttt ttattcagca
     3181 ctacaaaaaa gttaaggcaa ccgacgattt gatgaaagag ttttattacg aacaatcact
     3241 cgaactgttg aaaatgaaac agaaaataca agaacaattt agaacggctt ctgaaaagca
     3301 gaatacgggc taacaaagcg tgcacctgac gctggggatt ctgcggcaat ctcaagcag
//
LOCUS       DAOQJE010000335         3359 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_18298_length_3359_cov_4.767857, whole genome shotgun sequence.
ACCESSION   DAOQJE010000335 DAOQJE010000000
VERSION     DAOQJE010000335.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3359)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3359)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3359
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18298_length_3359_cov_4.767857"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1231)
                     /locus_tag="PLT08_09600"
     CDS             complement(<1..>1231)
                     /locus_tag="PLT08_09600"
                     /inference="COORDINATES: protein motif:HMM:NF013736.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HNE04760.1"
                     /translation="AMITALQWLVISRLKSIGVKAQKDLEERKKVQGQLEEAEARYRG
                     LVESVPLVIYIAEPGVMGKWHFVSPQITKLTGFSPEDWVKQPGLWYSRIHPDDRDRTI
                     QDETEALREGKMPRLEYRLLTRDEQYIWISDESYVFVDTSKLLVQGFMLDITSRKLAE
                     EQLTKRVAELQAVHGISEKLVQRTDLQKLIFDTGEQIRQSLKANNVLIAIHDPNTNLI
                     HFPYDFEEGVPRKDVPIHFGEGMTTYIMEHKIPVIIENEWDRRAGEMNVLNANSLPAK
                     SSVSVPIMTPERVIGVITIESTEHEYAFSESDSQLLMTIAANLAVAIEKTRLQESVKK
                     EMEIQEKLIRELEVKNEELERFTYTASHDLKSPLITIRGFLGYLEQDARAGKFERLVS
                     DIQRISDATEKMHRLLSE"
     assembly_gap    1232..1331
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1332..2420
                     /locus_tag="PLT08_09605"
     CDS             <1332..2420
                     /locus_tag="PLT08_09605"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNE04761.1"
                     /translation="NRVVHIPAGPEEPRSKSDIAKYIPEFVDGIRKFAEDKGIAYDVI
                     HSHYWMSGLAAEQLSDTWGGTPIVHMFHTLGEMKNRVARSEDEKAGEDRLNGERQVLR
                     RANRIVVATLAELTQLRFLYRADAEKMTVIPPGVDTEHFYPIPSDEAKQFIGLQPENR
                     MILFVGRIEPLKGVDTLIKAMSCLGIDTPNKERPVHLAIIGGEPDASPKDMSEEMSRL
                     QKICDDLCMGGMVVFLGKRAQDTLPYYYSAAEILVMPSLYESFGMVALEAMACGTPVI
                     ASEVGGLGYLVQDGVTGYTVPDSAPEALCEKLSILLGNSDLRNEMGMKAAEYATDYAW
                     EKIAAGIINVYEEVSTVHHIVDVKPPRT"
     gene            complement(2447..3196)
                     /locus_tag="PLT08_09610"
     CDS             complement(2447..3196)
                     /locus_tag="PLT08_09610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HNE04762.1"
                     /translation="MSEDSLRTLSRSKIAMLIDGDNAQAGLLSQMLVEAGRHGQVTLR
                     RIYGDWTTNNMNSWKDTLNFHAFQPIQQFRYTVGKNATDSAMIIDAMDILHSGVVDGF
                     CLVSSDSDYTRLATRIRETGIFVMGIGEKKTPKAFVNACDVFVYTENLVTAKKSTQQQ
                     KPNKGGAKKKDELDPIPLLTQAFEMAVQQDGWAALSNMGNALYQLDPAFDPRTYGHKQ
                     LSKMISKFKDRFEVRLQEIDGSTLFHVKLKE"
BASE COUNT          812 a          702 c          767 g          978 t
ORIGIN      
        1 attcactgag caaacggtgc attttttctg tcgcatccga tatacgttga atatctgaga
       61 ctaatctctc aaattttcca gctcgtgcat cctgttcgag gtaccctaaa aatccgcgaa
      121 tcgtgattaa tggagatttg agatcatgag aagcagtgta ggtaaagcgc tctaactcct
      181 cattttttac ttcaagctcg cgaatgagct tctcttgtat ctccatttct tttttcacag
      241 actcttgcaa acgtgttttt tcaatggcta cagccaggtt ggctgctatg gtcattaata
      301 actgcgagtc gctttcactg aacgcatatt catgctctgt gctttctatt gtgataacgc
      361 caatcacgcg ttcaggggtc ataatgggca cagaaacaga agattttgca ggtaaactat
      421 ttgcattaag aacattcatt tcacctgcac gccgatccca ctcattctcg ataataactg
      481 ggattttgtg ttccataata taagtcgtca taccctcacc gaaatggatg gggacgtcct
      541 tacgcggaac cccttcttca aaatcatagg ggaaatgaat taagtttgta ttggggtcgt
      601 ggatggcaat taacacattg ttcgccttca atgactggcg aatttgttcc ccagtatcga
      661 aaataagttt ctgtaggtcc gttctttgta ctaacttttc gctaataccg tgtacagctt
      721 gaagctctgc aacccgtttt gttagttgct cttctgccag tttccggctg gtgatatcta
      781 gcatgaaccc ttgtacaagt aatttgctcg tatctacaaa gacataactt tcatctgaaa
      841 tccaaatata ttgttcatct cgtgtaagaa gacgatattc taagcgcggc attttgcctt
      901 cgcgtaatgc ttctgtttca tcctgtatcg ttcggtcgcg gtcatccggg tggattcgcg
      961 agtaccaaag cccaggttgt ttgacccaat cttctggaga gaatcctgtc aattttgtga
     1021 tttgtgggct tacaaaatgc catttcccca taaccccagg ctctgcaatg tagattacaa
     1081 gtggcacgct ttcaacaagt ccgcgatatc gggcttcggc ttcttctagt tgtccctgaa
     1141 ccttttttcg ctcttctaga tctttttgtg ctttgacacc tatgcttttt agccgactaa
     1201 tgaccaacca ctgcaaagct gtgatcattg cnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1261 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1321 nnnnnnnnnn naaccgtgta gttcatatcc cagccggacc cgaagaacca cgcagtaaga
     1381 gcgatattgc caaatatatt cctgaatttg tggatggaat tcgtaaattt gccgaagaca
     1441 aaggtattgc ctatgatgtg attcatagcc attattggat gtctggactg gcggcggaac
     1501 aactgagtga tacctgggga gggactccca ttgtgcatat gttccacaca ttgggtgaga
     1561 tgaaaaatcg ggtggcgagg tcggaggatg aaaaagcagg tgaggatcga ctcaatggtg
     1621 agagacaggt tctccggagg gcgaaccgga tcgtcgtcgc tacattggca gaattgactc
     1681 agttgcgatt cctttatcgt gccgacgctg aaaagatgac cgtcattcct cccggcgttg
     1741 atacggagca tttttaccca atcccttccg atgaggcgaa gcagtttatt gggttgcagc
     1801 ctgaaaatcg aatgatcctt tttgtgggtc gtattgagcc actcaaaggc gtagatactc
     1861 ttatcaaagc catgtcgtgt cttggaatcg atacacctaa taaagaacgc cctgttcatt
     1921 tagcgatcat tggcggcgaa ccagatgcaa gcccaaaaga tatgtctgaa gaaatgtctc
     1981 gcctgcaaaa gatttgtgat gatctttgta tgggtggcat ggttgtgttt ttgggaaagc
     2041 gcgcacaaga tacattgcct tactattatt cagcagcaga aatattggtt atgccgtcgt
     2101 tatatgaatc gtttggaatg gtggcattgg aggcaatggc ttgcggcaca ccggtgattg
     2161 cttcagaagt gggcgggctt ggttatttag ttcaagatgg tgttactggc tacaccgtgc
     2221 ccgatagtgc cccagaggct ttgtgcgaaa aactttcgat ccttttggga aattctgact
     2281 tgcgaaatga aatggggatg aaagctgctg aatacgcaac cgattatgct tgggaaaaaa
     2341 tcgcagccgg gattatcaat gtatatgaag aagtttctac tgtacatcac atagtggatg
     2401 taaaaccgcc tcggacttaa ctcgaggcgg tttgctgctt ccaaacttat tctttcaatt
     2461 tgacatggaa caaggttgag ccatctattt cttgtaagcg aacctcaaac cggtctttga
     2521 atttactgat catttttgag agttgtttgt gtccgtatgt acgcgggtca aatgctgggt
     2581 ctaattggta taaggcattt cccatatttg aaagcgcagc ccaaccatct tgttgaaccg
     2641 ccatttcaaa ggcttgagtg aggaggggaa tcgggtctag ctcgtctttt ttcttcgctc
     2701 cgcctttatt gggcttttgc tgttgagttg attttttggc ggttacaagg ttttctgtgt
     2761 acacaaaaac atcacaggca ttgacgaagg ctttcggggt tttcttttct ccgatgccca
     2821 tcacaaaaat gccagtctcg cggatgcgcg tagccagcct agtgtagtcg ctgtcactgg
     2881 agactaggca gaaaccatct actacgccag agtgcaaaat atccatggca tcaatgatca
     2941 tggcgctgtc tgtggcgttc ttgccgactg tatatcgaaa ctgctgaatg ggttgaaagg
     3001 cgtggaagtt tagcgtgtct ttccacgaat tcatgttatt ggttgtccaa tcgccataaa
     3061 ttcgccgaag ggtcacctgt ccatgccgac cggcttcaac cagcatctga gagagtaatc
     3121 cagcttgggc attgtccccg tcaatgagca tggcaatctt gctacgagag agagttcgta
     3181 atgagtcttc tgacataatg aaattatacc tgtttgcgct ccatttctgc gcatggggaa
     3241 ggggcgttct agcgcctgta ttgggattac attactgaaa acttggctta agggtcctat
     3301 gataaaattt gaaatcttta tagcacagag gctttcatga aggagaaatt tctagcgct
//
LOCUS       DAOQJE010000336         3357 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_18316_length_3357_cov_5.618716, whole genome shotgun sequence.
ACCESSION   DAOQJE010000336 DAOQJE010000000
VERSION     DAOQJE010000336.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3357)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3357)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3357
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18316_length_3357_cov_5.618716"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..296
                     /locus_tag="PLT08_09615"
     CDS             <1..296
                     /locus_tag="PLT08_09615"
                     /inference="COORDINATES: protein motif:HMM:NF012360.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE04763.1"
                     /translation="IGSQVGKFEGSNVEIVEPVAIHASAKISNSKIGPYASIGANCKI
                     ENTQISESIIEPDCEIKDAALNHSLIGKQAKVKGRGDGHIMKLNVGDTSSIEL"
     gene            303..512
                     /locus_tag="PLT08_09620"
     CDS             303..512
                     /locus_tag="PLT08_09620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE04764.1"
                     /translation="MEAVECRSDTEYAERPLSLVWQGQRYEIAEIFSRWHGPGEKGFC
                     VKTTNGLAFELTYQEISDNWHVQPL"
     gene            522..1700
                     /locus_tag="PLT08_09625"
     CDS             522..1700
                     /locus_tag="PLT08_09625"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HNE04765.1"
                     /translation="MQEVNEVQRLSKRVAGLKPSGIRKFFDIAATMKDVISLGIGEPD
                     FTTPKPILDAGIRSLQNGETHYTSNSGKIELRQGVADNLKKLYNVKYDPANEIIATVG
                     VSEALYLTFTAVLEPGDEVIIPTPCFVSYQAEVILAGGVPVEIPARIENSFTVDPDDI
                     RKAITPRTKIIFIGYPSNPTGAVAPREVMVEIGKIAEEHNLLVVSDEIYDRLVYDFEH
                     VCFPALSDNLWHRTVLLGGFSKDYAMTGWRIGYACAPADLIKGLVRIHQYTIMSAPTT
                     AQDAAIEALKTGEPYVAEMVAEYDRRRRLLVDGLNRLGLTTFEPKGAFYTFPNIRASG
                     MDDETFAEKLLREESVAVVPGNAFGPGGEGFVRMCYATSYEQIEEALRRMERFMSRHG
                     "
     gene            complement(1748..2086)
                     /locus_tag="PLT08_09630"
     CDS             complement(1748..2086)
                     /locus_tag="PLT08_09630"
                     /inference="COORDINATES: protein motif:HMM:NF013422.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE04766.1"
                     /translation="MPENSRTVWSSDEGDQRKKTQTTTTHSRSLPPQQQTAYLHRESG
                     GRGGKVVSVVKNLVLTEEDMKTLAKKLKQECGTGGTVKDGIIEIQGEHRQRMAEVLTK
                     LGYKVKIAGG"
     gene            complement(2090..2965)
                     /locus_tag="PLT08_09635"
     CDS             complement(2090..2965)
                     /locus_tag="PLT08_09635"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNE04767.1"
                     /translation="MIKLTDVTKRYDNTTVVHPLNLQINEGEIVGIIGHNGAGKSTTM
                     KMIAGLVEPTSGEVRVMGQDMWKHNLKVKQRIGYLPEESPLYETMTARQYLLFFSELY
                     QMPRAKALSRIDGLLESLDLPQKDKLTGEFSKGMKRKTAIARTLLHNPDLLILDEPNS
                     GLDPLTSFFIINYLKTLKREGKTILLSAHNLFHVETICDRVAIIKNGRLLVFDTMDSI
                     RSRLGKREYQVIFHSDDKLEYEQLGGNYIFRTHDVDGIARMLETVSAKGWTLVDLSMR
                     ESALEEIYVKLMTNE"
     gene            3049..>3357
                     /locus_tag="PLT08_09640"
     CDS             3049..>3357
                     /locus_tag="PLT08_09640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE04768.1"
                     /translation="MNQILVIAWREITRLRKRFGGGGSPLTVLILLGVLGFTSYTLRD
                     SVTLGNGMYRVGVSGNVPAINDSRFAVMEVGVDEGQALLAEKKIDLLIVDAQVFSRED
                     D"
BASE COUNT          852 a          756 c          851 g          898 t
ORIGIN      
        1 agatcggttc acaggttggt aagtttgaag gctcgaatgt tgaaatcgtt gagccagtgg
       61 ctattcatgc cagtgcgaag atcagcaatt caaagatcgg tccgtacgct tcgattggtg
      121 cgaactgcaa gatcgaaaat actcaaattt cagaatccat catcgaaccg gattgtgaga
      181 taaaggacgc ggcgttgaat cattctttga ttggcaaaca agccaaggtc aagggaagag
      241 gcgatggtca catcatgaag ttaaatgtag gggatacgag ttcaatcgaa ctgtgagtcc
      301 gcatggaagc tgtagagtgc aggtcagata cagaatatgc ggaacgtccg ctttcgttgg
      361 tgtggcaggg gcagcgctat gaaattgcag aaattttctc gcgctggcac ggacccggtg
      421 aaaaaggatt ttgcgttaag accacgaatg gtctggcgtt tgaattgaca taccaggaaa
      481 tttcagacaa ctggcatgta caacctttat aaggaggctc aatgcaggaa gtaaatgaag
      541 ttcaaagact ttcaaaacgt gtggcgggtt tgaagcccag cgggattcga aaatttttcg
      601 atattgccgc aacgatgaag gatgtgatct cgctcggtat cggtgagcca gatttcacca
      661 cccccaagcc gattctagat gccggtattc gttcactaca aaatggtgaa acgcattaca
      721 cctcgaactc tggcaagatc gaattgcggc agggtgttgc agataactta aagaaacttt
      781 acaacgtgaa gtatgacccc gctaatgaaa tcattgcgac agttggggtt tctgaagcgt
      841 tgtatcttac cttcaccgct gtgctcgaac cgggtgatga agttatcatc ccgaccccat
      901 gttttgtttc ttaccaagcg gaagtgatcc ttgctggcgg cgtgcctgtg gaaattccag
      961 ctcgcattga gaatagcttt actgttgacc ccgatgatat tcgcaaagcg atcactccac
     1021 gcacgaagat tatctttatt ggttatcctt ccaaccccac cggtgcagtc gctccgcgtg
     1081 aagtgatggt ggagatcggc aagatcgcgg aagaacataa tttgttggta gtatcagatg
     1141 aaatttacga ccgcctcgtg tatgactttg aacatgtctg tttcccggca ttgagtgata
     1201 atctttggca tcgcactgta ttgttgggcg gtttctcaaa agactatgcc atgaccggtt
     1261 ggcgcattgg gtatgcttgt gctccagcag acctgatcaa aggactcgtt cgtattcatc
     1321 aatacaccat catgtctgcg ccaacaactg cgcaggatgc cgccattgaa gcgttaaaaa
     1381 ctggcgaacc ttatgtggca gaaatggttg cagaatatga ccgccgccgc cgtttattag
     1441 tggatggatt gaaccgcctc gggctgacga cctttgagcc gaagggcgcc ttttatacct
     1501 tccccaacat ccgcgcctct ggcatggacg atgaaacgtt tgccgaaaaa ttattgcgtg
     1561 aagaaagtgt ggctgtggtt ccgggcaatg ccttcggacc cggcggcgaa gggtttgtgc
     1621 gcatgtgtta tgccacttcg tatgaacaga tcgaagaagc tctgcgccgc atggaacgct
     1681 tcatgagtcg gcacggataa gtttataaat aaaaaaagag gacaggcatt gagcctgtcc
     1741 tcttttttta tccgccagca atttttactt tataaccaag cttggtcaaa acctctgcca
     1801 tgcgttgacg atgttcgcct tgaatttcga tgatgccatc tttcacggtt ccacctgtgc
     1861 cacattcctg tttgagcttc ttggcaaggg ttttcatatc ctcttcagtg agcacaaggt
     1921 ttttcaccac agaaaccacc ttccctcccc taccacccga ctcgcgatgt agataggcgg
     1981 tctgttgttg cgggggcaaa gatctagaat gagttgtggt cgtttgagtc ttcttacgct
     2041 ggtcgccttc atcagatgac caaacagtgc ggctattttc cggcatggtc tactcgttcg
     2101 tcattagctt gacatagatt tcttccaacg ccgattcgcg catggatagg tccaccaagg
     2161 tccaaccttt ggcagagacc gtttccaaca tgcgggcaat tccatctaca tcgtgagtac
     2221 ggaaaatata attcccgcca agttgttcat actctaactt atcatccgaa tgaaagatga
     2281 cttgatattc gcgttttccc aaccgtgacc gaatggagtc catcgtatcg aagactaaca
     2341 gtcttccatt cttgatgatc gcaactcggt cgcagatggt ttcgacatgg aagaggttat
     2401 gggcgcttaa caagatggtc ttaccttccc gtttgagcgt tttgagatag ttaataataa
     2461 agaaggaagt cagcgggtca agaccggaat tgggctcatc gagaataagc aagtcggggt
     2521 tgtgcaacag ggtgcgggcg atagcggtct tgcgcttcat acccttggaa aattcgccgg
     2581 tcaatttatc tttttgaggc aaatccaacg actcgagcag accgtcaatg cgagagaggg
     2641 ctttggcgcg cggcatttgg taaagctctg agaagaagag caaatattgg cgggcggtca
     2701 tcgtctcata cagcggactt tcctctggta aatagccaat acgttgcttg accttgaggt
     2761 tatgcttcca catatcctga cccatcacac gcacttcgcc tgaggtcggt tcgaccaaac
     2821 cagcgatcat tttcatggtg gtgcttttgc cagccccatt gtgcccgatg attccgacga
     2881 tctcaccctc attgatttgt aagttcaagg ggtggacgac tgtagtatta tcgtaccgct
     2941 tagtgacgtc tgtaagcttg atcatgatgc caatttatac caaacaaact caaggtaagg
     3001 ggtttgcaga agacagaaac ttttgcttcc ttacaaaagt ggaaacagat gaatcaaatc
     3061 cttgttattg catggcgtga aattacccgt ttaagaaaac gatttggggg tggcggcagc
     3121 ccgttgaccg tgctgatttt gctgggcgtt ctgggcttca cttcatacac tttgcgtgac
     3181 tctgtcacat taggcaatgg aatgtatcgt gtgggcgtct ctggaaatgt gcctgctatt
     3241 aatgacagtc gcttcgcagt gatggaagta ggcgtagatg aaggtcaagc cttattggca
     3301 gagaaaaaaa ttgacctgct gatcgtggat gcacaagtct tttcacgtga agatgac
//
LOCUS       DAOQJE010000337         3347 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_18422_length_3347_cov_5.540401, whole genome shotgun sequence.
ACCESSION   DAOQJE010000337 DAOQJE010000000
VERSION     DAOQJE010000337.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3347)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3347)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3347
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18422_length_3347_cov_5.540401"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1692)
                     /gene="ileS"
                     /locus_tag="PLT08_09645"
     CDS             complement(<1..1692)
                     /gene="ileS"
                     /locus_tag="PLT08_09645"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004822 - isoleucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006428 - isoleucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HNE04769.1"
                     /translation="MFKAVNPKLDVPSMEDNVLKMWTKQEIFKKTVDQRQGKPEYVFY
                     EGPPTANGRPGVHHVLARAFKDMFPRYKIMRGYHVSRRGGWDTHGLPVEIEVEKQLGF
                     TNKQQIEEYGVDKFNELCKKSVFNYIQEWEKLTGRIGFWVDLDTAYVTYTNDYIESVW
                     WILKNFWEKDLLFKGYKIVPYCPRCGTPLSDHEVALGYEDTADPSVFVRFPLVDKPDT
                     SFLVWTTTPWTLPANVAIAAHPDVDYVTVERDNNGTTEKLILAKSLLEKVFRGEEVKV
                     VDTFKGKKLKGMKYQPLFTFVPLDKPAHYVVLGDFVTTEDGTGLVHQAPAFGAEDMEM
                     AKQYDLPVLLTIQPDGGFIPEVTPWRGVFFKDADPQIIQNLEARGLMFRSEKYVHTYP
                     FCWRCHTPLLYYARDSWYIRTSAKKENLVNLNKTINWVPDHIKNGRFGNWLENNIDWS
                     LSRERYWGTPLPVWECENGECKHRECVGSVAELSEKAGRDLTELDLHRPHVDNVHWKC
                     VDCGGEMSRVKDLIDVWFDSGSMPYAQWHYPFENKDKFEAQYPADYICEAVDQTRG"
     misc_binding    complement(1762..2012)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(2059..>3347)
                     /locus_tag="PLT08_09650"
     CDS             complement(2059..>3347)
                     /locus_tag="PLT08_09650"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HNE04770.1"
                     /translation="SQKWLGRPFIIAAIIEPILISILLCTDRWHNLYFGGVRALNTTM
                     LVEMGPAAWANVVYSYLLILIGVLVLAVTLYRSHGVYRMQVTFILAATVTPWVIHILF
                     LSIGDHALLPDADVTPFIFSMTALLIAYALIRYRLLDVVPIARSILIESMSEGVCVLD
                     SQNRIVDINQTARKALSSEFFIGQPVEQAFKCWIDIVSHFFDQETAQTEVVIDDRYLD
                     LRISPVYDKNQRLLGKLVIWRDITELKNIQLKLQQLATIDELTLAFNRRHFMEIAEVQ
                     FSQALRQNQPFALALIDLDYFKGINDKYGHPAGDQVLAEFSRMFMENIRKFDVFGRLG
                     GEEFALLMPNTDIHFAYHASERLRFMITNTPIPINGGDVMVTFSLGVAMLHGAQDTLS
                     AIFRRADQALYAAKNSGRNQVVIWEQGLEAVAPKQA"
BASE COUNT          886 a          866 c          781 g          814 t
ORIGIN      
        1 gcctcgcgtt tgatcgaccg cttcacagat gtaatcagca ggatactgcg cttcaaactt
       61 atccttattc tcgaacgggt aatgccactg cgcatacggc atggaacccg agtcaaacca
      121 cacgtcgatg aggtccttca cgcggctcat ttctccaccg caatcgacac acttccaatg
      181 aacgttatct acgtgcgggc ggtgcagatc aagctcagtc aggtcgcgac cggctttttc
      241 agaaagctca gcaacagagc caacacactc gcgatgttta cattcgccgt tctcacattc
      301 ccaaacagga agtggcgtgc cccaatagcg ttcacgcgag agcgaccaat cgatgttgtt
      361 ctcaagccag ttgccgaagc gtccgttctt gatgtgatcg ggcacccagt tgatggtctt
      421 gtttaggttg acgagatttt ccttcttcgc gctggtgcgg atgtaccacg agtcgcgggc
      481 gtaatacagc aaaggagtat ggcaacgcca gcagaatgga tacgtgtgga cgtatttctc
      541 cgagcggaac atcagtccgc gcgcttcgag gttttgtatg atctgcgggt cggcatcttt
      601 gaagaaaact ccgcgccacg gcgtgacttc agggataaac ccaccatccg gttggatcgt
      661 gagcaagaca ggcaaatcgt actgcttcgc catttccatg tcttcggcac cgaaagccgg
      721 agcctgatgc acaagaccgg taccatcttc agtagtgacg aaatcaccga gcacgacata
      781 atgtgcgggt ttatcgagcg gcacaaaggt aaagagcggt tgatatttca tgcctttcaa
      841 cttcttgcct ttgaaggtat ccacaacctt tacttcttca ccgcggaaga ctttttccaa
      901 cagcgatttg gcgaggatga gtttttccgt cgtgccgttg ttatcacgtt cgacagtcac
      961 ataatccaca tcggggtgag ccgcgatggc aacattggca ggcaaggtcc acggagtagt
     1021 cgtccaaact aagaatgaag tatcgggctt gtccaccaat gggaagcgca caaaaactga
     1081 ggggtcagcc gtatcttcgt agccaagagc aacttcatgg tcagagagag gcgtgccaca
     1141 acgcgggcaa tacggcacga tcttgtagcc cttgaaaagc aagtccttct cccagaagtt
     1201 tttcaagatc caccacaccg actcgatgta gtcgttggtg taagtcacgt aggcagtgtc
     1261 taaatccacc cagaagccga tgcgaccggt gagcttctcc cattcctgga tgtagttgaa
     1321 gaccgatttt ttgcacagct cgttgaattt atcaacgccg tattcttcga tctgctgctt
     1381 gttggtgaag ccaagctgct tctctacttc gatctccacc ggtaagccat gcgtgtccca
     1441 gccgccgcgg cgcgagacgt gatatccgcg catgatcttg tagcgcggga acatatcttt
     1501 gaacgcacgc gcgagcacat gatgcacgcc ggggcgtcca ttggcggtgg gcggaccttc
     1561 atagaaaacg tactcgggtt tgccctgtcg ttggtcgacg gtcttcttga agatctcctg
     1621 cttcgtccac attttgagga cgttatcttc catcgaaggc acatcgagtt ttgggttgac
     1681 tgctttgaac attcttactc cttgtttccg tcatcatcca cgcaatgacg gtgaaattaa
     1741 aataaaaaac tctcatctca taacagagac gagagcaagg ctcacgcggt accactctga
     1801 ttttcatccg aagatgaaca ctcattggac gacaaccatc gccctgttct gataacgaag
     1861 aacaatttcg gattccctac tctcagaatt ttctgatttc agttcacagc tccgggagga
     1921 ttttcaaccg tcttgaacgt atccgcttca cacctgacct cggactcgct gcccgtcttg
     1981 aacggtttac tcgttcccat ccacgctttt agatagcggc attatacaca agccgcaggg
     2041 aatgagcaag gcgtgagttt aagcctgttt tggcgctact gcttccaacc cttgctccca
     2101 tatcactacc tgatttctgc cagaattctt cgcggcgtag agcgcttggt ctgctcggcg
     2161 gaaaatggca gagagcgtat cttgtgcgcc gtgtaacata gcaacgccca agctaaaagt
     2221 caccataaca tccccgccat tgatcggaat gggcgtattg gtaatcataa atcgtagccg
     2281 ttcagaagca tgatatgcaa aatgtatatc ggtgtttggc ataagcagag caaattcctc
     2341 cccgccaagg cgcccaaaca catcgaattt gcggatattc tccatgaaca tgcgtgaaaa
     2401 ctcagctaaa acttggtctc ctgcagggtg cccatactta tcatttattc ccttgaagta
     2461 atccaaatcg atcaacgcca aggcaaaggg ttgattttgc cgaagtgctt ggctaaactg
     2521 cacttcggca atttccataa aatgccggcg attgaatgcc agtgtcaatt catcgatggt
     2581 agcaagttgt tgaagtttca gctgtatatt ctttaattcc gtaatatcac gccagataac
     2641 caacttccct aaaaggcgtt gatttttatc atacacaggc gaaatacgta gatcaagata
     2701 tcgatcatca atcacaacct ctgtctgcgc cgtttcttga tcgaaaaaat gagagacaat
     2761 atctatccag cacttgaacg cttgttcaac tggctgtcca ataaagaatt ccgaagagag
     2821 cgccttccgt gctgtctgat taatgtcaac aatgcggttc tgcgaatcta agacgcaaac
     2881 cccttcgctc atagactcga tcagaatact gcgagcaata ggaacgacat ccaataaacg
     2941 ataacgaata agagcatatg caatgagtaa cgctgtcata gagaagataa atggcgtcac
     3001 atcagcatcg ggtaaaagag catgatcacc aatggaaaga aagaggatgt gaattaccca
     3061 cggcgtcaca gtcgctgcca agataaacgt cacctgcata cgatagaccc cgtgcgaacg
     3121 atacaacgtt acagccaata caagcacgcc aataagaatt agaagatacg agtagacaac
     3181 atttgcccag gcagccggtc ccatctcaac aagcatggtc gtgttcaaag cacgcacccc
     3241 gccaaaataa aggttatgcc agcggtctgt acacagcagg atggaaatca aaatgggttc
     3301 gatgatcgct gcaataataa aaggtcgccc cagccacttt tgcgaat
//
LOCUS       DAOQJE010000338         3345 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_18442_length_3345_cov_5.369301, whole genome shotgun sequence.
ACCESSION   DAOQJE010000338 DAOQJE010000000
VERSION     DAOQJE010000338.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3345)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3345)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3345
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18442_length_3345_cov_5.369301"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..690)
                     /locus_tag="PLT08_09655"
     CDS             complement(<1..690)
                     /locus_tag="PLT08_09655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE04771.1"
                     /translation="MKNPSRSYDLLWLSIALLPLIGLSFMLSVPAQDYWWYLRLGKDI
                     LEQGAIPLVDTMGYSRAGEPIFYQQWLSGIIFFLVYQAGNAPLTFLLRAVLIGITYGS
                     IWSLMRSYVGARTATILLIILGMATSNNWMIRPQLFAYPLFLLCLFAIYEWQKGNNRL
                     LFLLPLSTFLWANLHGSFVLPFLLAGASLVFGAPNIELRSKGFLSALRELGGSKKHQA
                     MFITILAMLIAS"
     gene            complement(733..1152)
                     /locus_tag="PLT08_09660"
     CDS             complement(733..1152)
                     /locus_tag="PLT08_09660"
                     /inference="COORDINATES: protein motif:HMM:NF019932.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HNE04772.1"
                     /translation="MDQEVHEFLLKHLQGIMTNDIMSYHETTSEDLTLYEWWVTPHRL
                     DGLPFHEFMMNSNAERGAVFGAEETGKTRFDLSNLLIQRYGDTAIASYTLLISTALGT
                     GVQVAAHNESRVMVKINGNWKVVHVHKSPAYSAPHVG"
     gene            complement(1155..2159)
                     /locus_tag="PLT08_09665"
     CDS             complement(1155..2159)
                     /locus_tag="PLT08_09665"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HNE04773.1"
                     /translation="MTRICITPFVQGTGGMASFRLKFEQGLRARNIDVTHDLDDKSDA
                     VLVIAGTRFILDLNRARRRGIRVVQRLDGVNWVQRVKWSGVKYSVRAEYGNVMLATIR
                     NRFADRVIYQSQFIRKWWEDWYGVAKSPASVIINGVDLNAYTHEGEHERPTDTYRMLL
                     LEGSLARGLNSGLFHAVSVAEKMSAKYPMEVVVAGVVDEATQQKLQSNVPVKFLGTVP
                     RADIPKLARSSHFMYCAEVNPPCPNSVIEAMACGLPVIGFDSGSLKELVTEDAGCIVP
                     YGANPWKLETPDIKTLASSAGEVLEKQNQFRAAARRRAGAAFGLDQMGESYLKVLLED
                     "
     gene            2248..2880
                     /locus_tag="PLT08_09670"
     CDS             2248..2880
                     /locus_tag="PLT08_09670"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013843.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019136 - deoxynucleoside kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HNE04774.1"
                     /translation="MYIAIEGVIGVGKTTLARLLQPAFEAEVLLEVFEENPFLSDFYG
                     DRARYAFQTQIFFLLSRYHQQNNNVPNILAAGKNLISDYTFAKDALFAGINIKGDELQ
                     MYHRVHEALGEKIPKPDLLVYLQATTDTLMSRIAFRDRPYERQMERAYIDELNVAYDE
                     FFSKPFDHTPILKIDTNDLNIIHNMEHIKLIENRIREALGLSPYQPALLK"
     gene            2890..>3345
                     /locus_tag="PLT08_09675"
     CDS             2890..>3345
                     /locus_tag="PLT08_09675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE04775.1"
                     /translation="MRVTKDSLLRIAKETVQERAYNDPDIVAAYLTGSLRGEPMLGGT
                     ADIDIVFVHKTTPMKVREFVKLTPDFHIDISRRAKDEFKSPRELRGDPWLGYEMYDPV
                     LLYEREKFFDFVQASLRAGFEFEQPPLMLQRCRTLLNHGRGNWMDISEHD"
BASE COUNT          869 a          820 c          811 g          845 t
ORIGIN      
        1 gctggcaatt agcatcgcga ggatggtgat gaacatggct tgatgttttt ttgaaccgcc
       61 aagctcgcga agtgcgctaa gaaaaccttt tgatctgagt tcaatattcg gcgcaccaaa
      121 gacaagagag gctcctgcca agaggaacgg caggacgaac gagccgtgta agtttgccca
      181 aagaaaagtg gacagcggta gaagaaagag caggcgattg ttcccctttt gccattcgta
      241 aattgcaaag agacataata agaataaagg atacgcaaac aactgcggac ggatcatcca
      301 attattcgaa gttgccatac ctagaatgat cagaaggatg gtagctgtcc gcgcgccgac
      361 ataggaacgc atcaatgacc agattgagcc gtaggtgatt ccaattaata cggcacgcag
      421 aagaaacgtg agtggtgcgt tgccagcttg ataaacaaga aaaaagataa tgcccgaaag
      481 ccattgctga taaaagatcg gctcgcctgc acggctgtag cccattgtat ccacgagcgg
      541 aatcgcgcct tgctccaaaa tatctttgcc caagcgcaga taccaccaat aatcttgagc
      601 agggacagac aacatgaaag aaagccctat cagtggaagc agggcaatgc tgagccagag
      661 gaggtcgtaa gagcgggaag gatttttcat aagattatgt aggggcgagg tcacctcgcc
      721 cctacgtgtt aattatccaa catgcggcgc ggaatatgcc ggtgacttat gcacatgcac
      781 aactttccaa ttgccattta tcttcaccat cacgcggctt tcgttatgtg ccgccacttg
      841 cacgccggtt ccgagtgcag tgctaataag caatgtataa cttgcaatag cagtatcgcc
      901 atagcgttgg atcaataaat tcgaaagatc aaatcttgtc ttgcccgttt cttctgcacc
      961 aaagaccgcg ccgcgctcgg catttgagtt catcatgaat tcatgaaaag gcaatccgtc
     1021 taatctgtgc ggcgtgaccc accattcgta taacgtcagg tcttctgatg tcgtctcatg
     1081 atatgacatg atgtcgttgg tcatgatgcc ttgcaaatgt ttcaaaagaa attcgtggac
     1141 ttcttgatcc atgattaatc ctccaataaa actttgagat acgactcgcc catttgatcg
     1201 agcccaaaag cggcccccgc ccgcctccgt gccgctgctc ggaattgatt ctgtttctca
     1261 agcacctctc ccgctgacga tgccagtgtc ttaatatctg gtgtttccaa cttccacggg
     1321 ttcgcgccgt acggaacgat acagcctgca tcctccgtaa ccaactcttt gagcgagccg
     1381 ctgtcaaaac cgatgacggg taaaccacat gccattgctt cgatgaccga gttggggcag
     1441 ggcgggttga cctcggcaca gtacatgaaa tgagaagagc gggcgagttt gggaatatct
     1501 gctcgtggga cggtgccgag aaatttaaca ggaacgttgc tttgtagttt ttgttgtgta
     1561 gcttcatcca ctacacccgc aactacaact tccattggat acttcgcaga cattttttcg
     1621 gctacgctta cagcatggaa caagcctgag tttaatccac gagcgaggct gccttcgagt
     1681 agaagcattc ggtatgtatc tgttgggcgt tcgtgctcgc cttcatgcgt gtaagcattt
     1741 aggtctacac cattgataat gaccgaggct ggggatttgg caacgccata ccaatcttcc
     1801 caccatttgc gaatgaactg actttggtaa ataaccctgt ctgcaaaacg attgcggatg
     1861 gttgccagca tcacattgcc atactcggcg cggacgctgt acttcacccc cgaccatttc
     1921 acgcgctgca cccagttgac cccgtctaac ctttggacga cgcgaatgcc ccgtcgccgt
     1981 gcccggttca ggtcaaggat gaagcgagtg cctgcgatga cgagcaccgc atcagacttg
     2041 tcatccagat catgagtcac atcaatgttg cgggcgcgta atccctgctc gaatttcaaa
     2101 cggaaggatg ccatgccgcc tgtcccttga acgaatggcg tgatgcaaat gcgagtcata
     2161 tacaaaatta taaccgtagg ggcgaggtca cctcgccctt acttttatgg tatcctatgt
     2221 tcaatcattt tttgggagaa tttgttcatg tatattgcaa ttgagggcgt gatcggtgta
     2281 gggaagacta cacttgcgcg cttgctgcaa cctgcttttg aagcagaagt gttattggaa
     2341 gtttttgaag agaatccgtt tctctcagat ttttatggcg accgcgcacg ttatgcattt
     2401 caaacccaga tcttcttttt gctcagtcgc taccatcaac agaacaacaa tgtgcccaat
     2461 attcttgcgg ctggaaaaaa tttgatctct gattacacct ttgccaaaga tgcgcttttt
     2521 gcaggcatca acataaaagg tgacgagttg cagatgtatc acagagtgca tgaggcgctt
     2581 ggcgagaaaa ttcctaaacc cgaccttctg gtttatctgc aagcgacgac cgatacgttg
     2641 atgagccgta ttgcatttcg cgaccgcccg tatgaacgcc agatggaacg tgcctatatc
     2701 gatgaattga atgttgcgta tgatgaattc ttctcgaagc cgttcgacca tacgccgatt
     2761 ttgaaaattg atacgaacga tctaaatatc atccataata tggagcatat taagttgatc
     2821 gaaaatcgca tccgtgaagc tttggggctg tcaccttatc agcccgcctt gttgaaatag
     2881 gcaggaccca tgcgcgttac aaaagactca cttttacgca ttgccaaaga aaccgttcaa
     2941 gaacgggcat ataacgatcc cgatattgtt gcggcatatc tcaccggctc gttgcgcggc
     3001 gaacccatgc ttggcggcac tgccgacatt gacattgtct tcgtccacaa aaccacgccg
     3061 atgaaggttc gtgaattcgt caaactcacg cccgattttc acattgatat atcccgccgt
     3121 gcaaaggatg aattcaaatc tccgcgtgag ttgcgcggtg atccgtggct cgggtatgaa
     3181 atgtatgacc cggttctgtt gtatgaacgc gaaaagtttt tcgattttgt gcaagcaagc
     3241 ctgcgcgctg gctttgagtt tgaacaaccg ccgttgatgt tgcagcggtg tcgcacactt
     3301 ctcaaccacg ggcgcggcaa ctggatggat atttcggaac acgat
//
LOCUS       DAOQJE010000339         3343 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_18465_length_3343_cov_4.530109, whole genome shotgun sequence.
ACCESSION   DAOQJE010000339 DAOQJE010000000
VERSION     DAOQJE010000339.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3343)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3343)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3343
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18465_length_3343_cov_4.530109"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(231..2060)
                     /locus_tag="PLT08_09680"
     CDS             complement(231..2060)
                     /locus_tag="PLT08_09680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE04776.1"
                     /translation="MGREISKVKEASMNDKSQDGIFFVNINEEHKRGHNKDTVYALRS
                     SKYSKRFKIYQETPKTPDSILQSVLEGILTSDAVLLDAIFDKQESFKDRLIQFGIAFA
                     LEKQRFFFYIQDNSSRESKLISEYIEAFTVEALGYLHFTDLLNNKTEVLISQPQPKPQ
                     KPTEKPYQSFSVIGVNETTNPDLIKVIKDFAGKKSWHASFYKPLSGANIHDELSRHVV
                     TRPFCVFCVNQHTDTNTYIAIGLALGFGIPFLILIEKGDTFPQILNGYAGVVVYENNT
                     DLLADLEKYTSIFYSPENFKSWEGFTYFYLLSKTEKRLIEADKVSEIEEIEKVILAVS
                     KVGRAPILLSYILLGDVYRKKNQIIDPLNTEFLMQAISWYEKALKIQEDNIRCIDGID
                     ATKKLIKVIDLVKDKNYESIPELIYLIGNGINSEQYQYLKSFLILEVKKLFTNEEYLP
                     AIALLASMQKHDKSEELSKLWEEVNPKKFLESIQKYQENELKIKSELRDALKKNKDVD
                     NQLSNAYKEVEQANQIRAKLEDTKNFYGKVIFVNFGRGWATYTPIKGLPFIQRGDQLI
                     NAKEGMGIQGGDTVFDEDGNAQFHWLSKEENLALAYLQSTNSS"
     gene            complement(2036..3046)
                     /locus_tag="PLT08_09685"
     CDS             complement(2036..3046)
                     /locus_tag="PLT08_09685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE04777.1"
                     /translation="MSEPISENNIYAKLGLNIPLTERETIIRNLIETHKTDTLFTDGG
                     KCSNVPLQVYPNRYFLAQNFDKKKEDMRNALQEAFDEFNLKVITVDEVIGGALFCNIA
                     SLILGTPFGIYHIDKEEKPNVYLELGVSIGLQKPFVLVKDKHTGIASVLEFVQYYEMS
                     DYFSLSETLGNLTNEYITRIGYFDSALMQKASMKTREVCISLGELESVDIGVTLAIQI
                     VKRGYHPVFLCETNKNLAKFLEQKEIEPVFYHKLPEVSNAIARSKFGIYRVDASASST
                     SFIYLGIAIGLNRPTLMINNEREKPPSDLSIFDALKFAGRTDLEEKFELAFPKWEERF
                     LK"
     gene            3096..>3343
                     /locus_tag="PLT08_09690"
     CDS             3096..>3343
                     /locus_tag="PLT08_09690"
                     /inference="COORDINATES: protein motif:HMM:NF017822.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DpnI domain-containing protein"
                     /protein_id="HNE04778.1"
                     /translation="MVVFIKDTQNEIVNGKKHCNLYEFFPKHPRDDGRVGKSVWVLSV
                     LRGGLSRFENNAPVADFYCGHCSEEYELKSKSGAMGKK"
BASE COUNT          975 a          617 c          601 g         1150 t
ORIGIN      
        1 tccatttcat attgttgcgg aatccataaa ccgccaataa gcatatagcg ggcgttatgg
       61 atattgcttt catcgcagta gagtgagtat cgcgtttttt ccataatcta tttgaaggtc
      121 caccctgtaa tgggagtata taatggcatt atagcacata cgttcacata gcaagtccat
      181 gaggagaatt tgagggggta gtgtttgcga ggatttcttc caaaatatat ctacgaagaa
      241 tttgtgcttt ggagataagc caatgccaag ttttcttctt tacttagcca atggaattgt
      301 gcattcccat cttcatcaaa aactgtatct ccaccttgaa tgcccattcc ttcttttgca
      361 tttattaact gatcacctct ttgtataaat ggtaatccct ttataggagt ataagtagcc
      421 cagcctcttc caaagttaac aaatataact ttgccataga aattctttgt atcttcaagt
      481 ttagccctaa tttgatttgc ttgctcgact tctttatacg cattacttaa ctgattatct
      541 acatccttgt tctttttaag agcatccctt agctcacttt tgattttcaa ctcattctct
      601 tgatatttct gaatcgactc taggaacttc ttagggttta cttcttccca tagtttactt
      661 aattcttcag atttatcatg tttttgcatt gaggcgagta atgctattgc aggaagatac
      721 tcctcatttg taaacaactt tttaacttca agaatcagaa acgactttag atattgatat
      781 tgttcggaat taataccatt gccgattaga taaattaatt caggtattga ttcatagttc
      841 ttatctttaa ctaagtcaat caccttgatt aattttttgg ttgcatcaat tccatcaatg
      901 catcttatat tatcttcctg aatttttaat gctttttcgt accaagaaat tgcttgcatt
      961 aagaattcgg tgtttagagg atcaataatc tgattttttt ttcgataaac atcacccaat
     1021 aaaatatacg ataaaagaat tggcgctcgc ccaactttgg atacagcaag aataaccttt
     1081 tcgatctctt cgatttctga tactttatca gcttcaatca agcgcttttc tgttttagag
     1141 agtaaataga aataagtaaa tccctcccat gatttgaagt tttctgggga atagaaaatt
     1201 gaagtgtact tttctaagtc ggcaagtaaa tctgtattat tttcataaac aacaacacct
     1261 gcgtacccgt ttagtatttg aggaaacgtg tcgccttttt caatcagtat taagaatgga
     1321 attccaaagc ctaaagctaa accaatggca atatatgtat ttgtgtcggt atgttggttt
     1381 acacaaaaaa cgcaaaatgg tctcgttaca acgtggcgag acagttcatc gtgtatattt
     1441 gctccagaaa gtggtttgta gaaagaggca tgccaacttt ttttacctgc aaaatctttt
     1501 ataactttta tcagatctgg atttgtagtt tcgtttacgc ctattacgga gaatgattga
     1561 tagggttttt ctgttggttt ttgcggttta ggctgtggtt gagaaattaa tacttcagtt
     1621 ttattgttta acaagtcggt aaaatgtaag taccccaacg cttctactgt aaatgcctct
     1681 atatactctg agattaattt gctttctcta gaagaattgt cctgaatgta gaaaaaaaac
     1741 ctttgttttt caagggcgaa agcaatacca aattgtatca acctatcctt gaaagactct
     1801 tgtttatcaa aaatcgcatc tagtaaaaca gcgtcagatg taaggatacc ttcaagtact
     1861 gattgcaaaa tcgaatctgg ggttttcgga gtttcttgat aaattttaaa gcgcttggaa
     1921 tatttagaac tccttaaagc ataaactgtg tctttattat gtccgcgttt atgctcttca
     1981 ttgatgttga caaaaaaaat cccatcctgg ctcttgtcat tcatagaagc ctcttttact
     2041 ttagaaatct ctcttcccat ttggggaagg ctaactcaaa cttttcttct aaatctgttc
     2101 gccctgcgaa ttttagtgcg tcaaaaatgc ttaaatcact cggaggtttt tcgcgttcat
     2161 tgtttatcat gagtgtgggg cgatttagcc caatagcaat tcctagatag ataaatgaag
     2221 tcgatgatgc agaagcgtca acccgataaa tgccaaactt tgaacgagca attgcattac
     2281 tgacttcagg cagcttatga taaaaaacag gttcaatttc cttttgttca aggaatttgg
     2341 ctaagttttt attcgtttca cagagaaaaa cgggatgata cccacgtttg actatctgaa
     2401 ttgcaagtgt cactccgata tctacagatt caagttcccc caaagaaatg cacacttccc
     2461 ttgttttcat tgaagctttt tgcattaatg cgctatcaaa gtaacctata cgggtaatgt
     2521 actcatttgt cagatttcct agcgtttctg acaaactaaa ataatcgctc atctcgtaat
     2581 attggacgaa ctcaagaaca gatgcgatgc cagtgtgctt gtctttgaca agcacaaagg
     2641 gtttctgtag cccaatggaa actcctagtt cgaggtaaac attaggtttt tcctctttat
     2701 cgatatgata tattccaaaa ggagttccta gtatcaatga tgctatgttg caaaacagtg
     2761 ctccaccaat cacttcgtca acagtaatta cctttaaatt gaattcgtca aacgcttcct
     2821 ggagggcatt tctcatgtct tctttctttt tatcaaaatt ttgcgctaaa aagtatctat
     2881 tgggataaac ttgaagagga acgttgctgc attttccgcc gtctgtaaac aaggtgtcgg
     2941 tcttgtgagt ctcaataaga tttctaataa ttgtttctcg ctcagtaagc ggaatgttta
     3001 atcctagctt ggcataaata ttattttctg aaatcggctc tgacatttta tagacctccg
     3061 caaaagacta attcatatta tacaggtgtt taattgtggt tgtattcata aaggacacac
     3121 aaaatgaaat tgttaatgga aaaaaacact gtaacttata tgagtttttc ccaaaacatc
     3181 cgcgtgatga cggaagggtg ggtaaatcgg tctgggtttt gtccgtgttg cggggtgggt
     3241 taagtcgctt cgagaacaat gctcccgtcg ctgattttta ttgcggtcac tgttcggaag
     3301 aatacgaatt gaaatcaaaa agcggggcaa tgggcaagaa aat
//
LOCUS       DAOQJE010000340         3342 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_18480_length_3342_cov_6.323699, whole genome shotgun sequence.
ACCESSION   DAOQJE010000340 DAOQJE010000000
VERSION     DAOQJE010000340.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3342)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3342)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3342
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18480_length_3342_cov_6.323699"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(126..998)
                     /locus_tag="PLT08_09695"
     CDS             complement(126..998)
                     /locus_tag="PLT08_09695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein"
                     /protein_id="HNE04779.1"
                     /translation="MKRVIVFLLLLSILLISCGSSSTDTSSDSPAQYIENKGSDTIVN
                     LALAWAETYQAEFPDVRISVTGGGSGTGIAALINGTVDIANASRKIKDEELTEAQSKG
                     VDPLEHIIARDAIAVIVNPENPVNELTLQQIADIYSGKITNWREVGGEDRPIVKLSRE
                     TNSGTHVYFLETVLRLGDKENKTLFSTDTLLLPSSEGIIAEVRQNPNAIGYDGLGYVP
                     DDLKMIAIAKETGGTYVLPAIATVNDKTYPIARDLYMYTNGEPTGAVKEYLDWILSPE
                     AQEIVAKLGFVPVK"
     gene            1115..1876
                     /gene="pstB"
                     /locus_tag="PLT08_09700"
     CDS             1115..1876
                     /gene="pstB"
                     /locus_tag="PLT08_09700"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003101281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HNE04780.1"
                     /translation="MSAPQPAFTVQNMDFYYNTSKKTLSNINLEIQPQKVTALIGPSG
                     CGKSTFLRCLNRMNDTIPGTKVDGKIFLNGEDIYGETMDVVTLRQRVGMVFQKPNPFP
                     QSIFDNVAFGPRVMGMDVKLSEVVEKSLRAAALWDEVKDDLKSDAMGLSLGQQQRLCI
                     ARVIAVQPEVILMDESTSALDPIATLRVEELIAELKKQYTIVIVTHNMQQAARVSDYT
                     GFFWLGEMVEFAQTNQMFTVPKQELTEAYITGRMG"
     gene            complement(1975..2664)
                     /gene="phoU"
                     /locus_tag="PLT08_09705"
     CDS             complement(1975..2664)
                     /gene="phoU"
                     /locus_tag="PLT08_09705"
                     /inference="COORDINATES: protein motif:HMM:TIGR02135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0051049 - regulation of transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HNE04781.1"
                     /translation="MIRKTFESDIQQVKDEVLALGSMVEHAIISSVEALKKRDIKAAE
                     KIIAEDKEINKKRFAIENQLMILIATQQPMAHDLRLLASTMEIISELERMGDYAKGIA
                     NINVRMADEKLLKPLIDIPRMAEKGASMLHRALTAFVHEDIETAKSLPIEDDEVDALY
                     NQIYRELMIFVIEDPKNIERANWLLWVAHNLERVADRVTNICERTVFIATGEFGEIKS
                     SDDEFWKNQNK"
     gene            complement(2810..>3342)
                     /locus_tag="PLT08_09710"
     CDS             complement(2810..>3342)
                     /locus_tag="PLT08_09710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004810563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HNE04782.1"
                     /translation="DPVEVRRKIGMVFQKPNPFPKSIYENIAWGARINGFKGNMDELV
                     ESSLRSAALWDEVKDKLQQSAYALSGGQQQRLCIARTIAIQPEIILMDEPASALDPIS
                     TLRIEELMQELKKNYTILIVTHNMQQAARASDYTAMMMIDDQRAGGVIEFDDTKNIFT
                     RPKDKRTEDYVTGRFG"
BASE COUNT          759 a          741 c          897 g          945 t
ORIGIN      
        1 acgatggcgg aatatccgct cgctttgatg gctcgggtga tgataaattc tcgccagttt
       61 aaagattttt ccatagtgct ccatcgtcat tgcgagggtg aagatcatcg caatgacgaa
      121 atgatttatt tcacaggcac aaacccaagt tttgccacaa tctcttgcgc ttcaggggag
      181 aggatccaat cgagatattc tttcacagcg ccagtgggtt caccattggt atacatataa
      241 aggtcacgcg caatgggata ggttttgtcg ttcacggtag caatggcagg taagacataa
      301 gtgccgcctg tttcttttgc aattgcgatc atcttgaggt catctggaac atagcccaag
      361 ccatcgtacc caatcgcgtt cgggttttgc cttacttcgg caatgatgcc ttctgaagac
      421 ggaagtagca aggtgtctgt tgagaacaac gttttgtttt ctttgtctcc caatcttaag
      481 acggtttcca aaaagtaaac atgggtgcct gagttggttt cgcgtgaaag ttttacgata
      541 ggtcgatctt cgccgccaac ttcacgccag ttggtgatct tgccgctgta aatatctgcg
      601 atctgctgca acgtcaattc gttgacgggg ttttctgggt tcacgatcac ggcaatcgca
      661 tcgcgtgcga tgatgtgctc aagcggatcc acccctttgg attgggcttc ggtcaactct
      721 tcatctttga ttttgcgtga agcgttggca atatccacgg ttccgttgat gagcgcggca
      781 atgcctgtgc ctgaaccacc gcctgtcacc gagatgcgca catcggggaa ttcggcttga
      841 taggtttctg cccatgccaa cgccagattg acgatggtat cggagccttt gttttcgatg
      901 tattgcgcgg gggagtcaga gcttgtgtca gtggatgaag aaccacaaga gattaataga
      961 atggaaagaa gaagcaagaa gacgattaca cgtttcatcg gctgggatta taccgcctca
     1021 aacttgacgg aggcttgatt ctgaccctga cgctttttgc cactttatga atttttttta
     1081 ccgctttgat tttccttttt gatacaatca aatcatgagc gcaccccagc ctgcctttac
     1141 cgtccagaac atggactttt actataacac atcaaaaaag accctctcga acatcaacct
     1201 ggagattcag ccccaaaagg tgacggcgtt gatcggtcca tcgggttgtg gaaaatctac
     1261 gttcttgcgt tgtcttaacc gcatgaatga caccattccc ggcaccaaag tggatgggaa
     1321 gatctttctc aatggtgaag atatttatgg cgaaacgatg gatgtggtga cacttcgtca
     1381 gcgggtgggg atggtcttcc agaagccgaa cccatttccg caatccattt tcgataacgt
     1441 ggcgttcggt ccgcgggtga tggggatgga tgtgaagctc agcgaagtcg tggaaaaatc
     1501 tctgcgggcg gcggcattgt gggatgaagt aaaagatgat ttgaaatcag acgcgatggg
     1561 cttgtcattg ggacagcaac aacgtctgtg cattgcgcgc gtcattgctg tgcaaccgga
     1621 agtgattctg atggatgaat cgacttcggc attggacccg attgccacac tgcgcgtgga
     1681 agaactgatc gcagaattaa agaagcagta cacgatcgtg atcgtgaccc ataacatgca
     1741 acaagctgcg cgtgtttctg attacaccgg ctttttctgg ttaggagaga tggttgagtt
     1801 tgcgcagacc aatcagatgt ttacggtgcc aaagcaagaa ttgacagaag cgtatattac
     1861 cggaagaatg ggctaattag tctggtagtt tgttagtcga atagccttgt agttattaaa
     1921 caaagcgagt ggatgagttt tctcatccac tcgcttttta tttcttgtaa cgtattactt
     1981 attctgattc ttccagaact catcgtcgct ggatttgatc tcgccgaatt caccggtggc
     2041 gatgaagacg gtgcgctcgc agatgttggt aacacggtct gcaacgcgct cgaggttgtg
     2101 ggcaacccac aacagccagt tggcgcgttc gatgttcttg gggtcttcga tcacgaagat
     2161 catcaactca cgatagatct ggttatagag tgcgtccact tcgtcatctt cgatggggag
     2221 ggacttggca gtctctatat cttcatgcac aaaggcggtt agggcgcggt gcagcatgct
     2281 ggcgcctttc tctgccatac gtggaatgtc gatcagcggc ttgagcaatt tctcatctgc
     2341 catgcggacg ttgatgttgg caatgccttt ggcgtaatcg cccatgcgtt caagctcaga
     2401 gatgatctcc atggtggagg cgagcaggcg caggtcgtgc gccatgggtt gttgggtggc
     2461 aatgaggatc atcagctggt tttcgatggc gaaacgtttt ttgttgatct ctttatcttc
     2521 ggcaataatt ttttcagcgg ctttgatatc gcgctttttt aacgcttcca ctgagctgat
     2581 aatggcatgt tccaccattg agccgagtgc cagcacttca tcttttacct gttgaatgtc
     2641 gctctcaaaa gttttacgga tcataagtcc tctttaggtt aattgtgttc attttactac
     2701 ttttgcgatt atcgattcta tctgacggtg ccgtttcgga agggtccgct caaggggaga
     2761 gaaaggtttg ggcgggcgaa caggcaaaac gtctgaaaga ggcggctttt tatccaaatc
     2821 ttccggtgac gtaatcctcg gtccgcttgt ctttggggcg ggtgaagatg ttcttggtat
     2881 cgtcaaattc gatcacgccg ccggcacgtt ggtcgtcgat catcatcatg gcggtgtagt
     2941 cgctggcgcg tgcagcttgt tgcatgttgt gagtgacgat gagaatggtg tagttctttt
     3001 tcaactcttg catcaattct tcgatgcgca gcgtggagat cgggtccagc gcagaggcag
     3061 gttcgtccat caagatgatt tcgggttgaa tggcgatggt gcgtgcaatg cagagacgtt
     3121 gttgctgacc acccgagagg gcataggcgc tttgttgaag tttgtctttt acttcatccc
     3181 aaagggcggc ggaacgtaga gagctttcaa cgagttcgtc catattgccc ttgaatccat
     3241 tgatgcgagc gccccaagcg atgttctcgt aaatggattt gggaaagggg ttcggttttt
     3301 gaaagaccat gccgatcttg cggcgcactt ccacggggtc ta
//
LOCUS       DAOQJE010000341         3337 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_18511_length_3337_cov_5.462828, whole genome shotgun sequence.
ACCESSION   DAOQJE010000341 DAOQJE010000000
VERSION     DAOQJE010000341.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3337)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3337)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3337
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18511_length_3337_cov_5.462828"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..704
                     /locus_tag="PLT08_09715"
     CDS             <1..704
                     /locus_tag="PLT08_09715"
                     /inference="COORDINATES: protein motif:HMM:TIGR01065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HNE04783.1"
                     /translation="APMSSQKPALDPMISSPDSNEYFNSISHLIGAILSISALAVLVS
                     LAAIAGKPAHVVGFAIYGTSLFLSFLFSCLLHFFLLFNKYYRVFGILDHNAIYILIAG
                     TYTPFCLTIFSGATGWVLFGVIWSLAVFFITLKSIFFAKIPVWLSQASFLLMGWIVVF
                     LIKPAYTQLGMDAIILMIAGGLSYTVGAISFAIGKPNPFPPYFGNHEIWHICVLAGNT
                     FMFLVMLWHILPYPK"
     gene            complement(705..1331)
                     /locus_tag="PLT08_09720"
     CDS             complement(705..1331)
                     /locus_tag="PLT08_09720"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HNE04784.1"
                     /translation="MTIRIMIVDDHAIVRSGLKAILASQQDFELVADVGTGDAAVSAS
                     ATLRPDVILMDLQMPVMDGVTAISLIHQKQPDIRMLVLTTYDTDADIVRAVEAGAIGY
                     LLKDALPDELFRAIRSAARGEVIFAPSVAAKLAKRLTNTLDRSLSNREIEVLILAARG
                     DSNKEIANKLHITEATVKSHFVHIFNKLSVTDRTAAVTLALEKKLIRL"
     gene            complement(1328..>2019)
                     /locus_tag="PLT08_09725"
     CDS             complement(1328..>2019)
                     /locus_tag="PLT08_09725"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HNE04785.1"
                     /translation="LISQSSQRYRLLNELEETRFNLLKAERETAILSERQRLSREIHD
                     TLAQDFTSIIMSLNAAKLTENSTAQVYLEQAEQTARDGLNDARRIVWALRLEELENNS
                     FTEAIERLTARWSAETAIRVNSRFTGNPKPMRAEHEAALLRVTQEALHNIKKHAHAQE
                     VNITLSYMPDLFALDIADNGIGFEQRNLKGFGLKTMRERIEEIGGSLVIESQFGAGTT
                     IAVSLPLEEQG"
     assembly_gap    2020..2119
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2120..2661)
                     /locus_tag="PLT08_09730"
     CDS             complement(<2120..2661)
                     /locus_tag="PLT08_09730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE04786.1"
                     /translation="MKQNSMQHAWQQWAWVWHAIAYIMMALNLAIALENSLRRGSFQT
                     TAWLTILLAVWYLPFMALPARQSINTPWKLQLYFIIGWVIWMALIYFHNPAMMLAALF
                     YPQVFIRLPFRWAMVSAVLFTVSTFYLGFILPATPDALPTLYVVGVLLVSSQAILGGF
                     INSLISQSSQRYRLLNELEET"
     gene            complement(2708..>3337)
                     /locus_tag="PLT08_09735"
     CDS             complement(2708..>3337)
                     /locus_tag="PLT08_09735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE04787.1"
                     /translation="NRVVAVAGPLVSFLTGLIALGYFYRNQRTDASLTFFLWHFATVN
                     LMMASGYFLFSGVAGIGDLGTGESGAFQGVQPEWLIRMGLSVLGLVFYYAVIRISIKM
                     MDHFIGGEGRERVGRAQMLSVIAYLVGGGIAILIGLFNPYGIVIVLVSSFASSMGGTS
                     GMAWMMQLLDRSKDTGMPPFAMPRNWSWIFVGVGFILLYAIIFGRTLYL"
BASE COUNT          883 a          816 c          690 g          848 t
ORIGIN      
        1 tcgcgcccat gtcttcacaa aaacccgctc tcgaccccat gatctcgtcg ccagatagca
       61 acgagtattt caactccatc agtcatctca tcggtgcgat cctttcgatc tctgctttgg
      121 cagttttggt ttcgctggca gccatcgctg gcaagcccgc ccatgtggtg ggatttgcca
      181 tctacggcac gagtttattt ctatcgttcc tattcagttg tttactgcat ttctttttac
      241 tgttcaacaa gtattaccgc gttttcggca tccttgacca taatgccatt tatattttga
      301 tcgcaggcac gtacacacct ttctgtctca ccattttcag cggtgcgaca ggttgggtgc
      361 tttttggcgt catttggagc ttggcggttt tcttcatcac actcaaatct attttcttcg
      421 ccaagatccc ggtctggctt tcgcaggctt cattcttgct catgggttgg atcgtggtct
      481 ttctaataaa gcctgcctat actcaactcg gcatggatgc gattatcctt atgattgcgg
      541 gcgggttgag ctacaccgtg ggtgccatca gctttgccat tggtaagccc aatcctttcc
      601 caccgtattt tgggaatcat gagatctggc atatctgcgt cttggctggg aacacgttta
      661 tgtttcttgt gatgctatgg catatactgc cctaccccaa ataactacaa ccgaatcaat
      721 ttcttttcaa gcgccagcgt tacagccgca gtacggtctg tgacgctgag tttgttaaag
      781 atatgcacaa aatgcgattt aaccgtagct tctgtgatat gtagtttgtt agcaatttct
      841 ttattggagt caccgcgggc cgccagtata agcacttcaa tctcgcgatt actaagagac
      901 ctatcaagcg tattcgttaa gcgtttcgcc aatttagcgg caacactggg agcaaaaatc
      961 acctcgccac gcgcggcaga acgaattgcc cgaaacaatt cgtcaggtaa tgcatctttc
     1021 aatagatacc ctatcgctcc agcttcaaca gcccgtacaa tatcggcatc ggtatcatat
     1081 gttgtaagaa caagcatacg aatatctggc tgtttttggt gaataagcga aattgccgtc
     1141 accccatcca taactggcat ttgcaaatcc atcaaaatca catcggggcg aagagtggca
     1201 gaagcactaa cagcggcgtc tccggtccca acgtcagcga ccaactcaaa gtcttgttgt
     1261 gaggcgagga tcgctttcaa tccactccgc acaatcgcat ggtcatccac aatcataatg
     1321 cgtatggtca tccttgctcc tcaagaggca aagagacggc aattgttgtt cccgcaccga
     1381 attgactttc gatcacaaga gagccaccta tttcttctat ccgttcacgc atggttttaa
     1441 gaccaaagcc cttaagattt ctttgttcaa acccgatgcc attatcggca atatccaacg
     1501 cgaataaatc tggcatatac gaaagcgtga tattgacttc ttgcgcatgg gcatgtttct
     1561 taatgttatg caatgcttct tgcgtgacgc gcaaaagcgc cgcctcatgc tccgctctca
     1621 tgggcttggg attaccagta aaccttgagt ttacgcggat ggctgtttca gcagaccagc
     1681 gagctgttaa tctttctatc gcttctgtaa atgaattatt ctcaagctct tccaagcgta
     1741 atgcccacac aatacgacgc gcatcgttta agccatcgcg tgcggtttgt tcggcttgct
     1801 ctaaatacac ctgtgccgtg ctgttttcag ttaacttagc agcgttgaga ctcataatga
     1861 tactggtgaa gtcttgagca agcgtatcgt gaatttcacg tgataatctc tggcgctcgc
     1921 tcaagattgc agtttcacgt tcagccttta ggaggttaaa gcgcgtctcc tcaagctcat
     1981 tgagcagacg ataccgttga ctgctttgtg agatcaaggn nnnnnnnnnn nnnnnnnnnn
     2041 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2101 nnnnnnnnnn nnnnnnnnng tctcctcaag ctcattgagc agacgatacc gttgactgct
     2161 ttgtgagatc aaggagttaa tgaagccgcc aagaatagct tgcgaagaaa ccaataaaac
     2221 cccaacaaca tacagggttg gcaaagcatc aggtgtggca ggcagaatga acccgagata
     2281 aaacgtgctt accgtgaaca acacagcact gaccattgcc cagcgaaatg gcaggcggat
     2341 aaaaacttgt gggtaaaaca aagccgccaa catcatagcc gggttatgga agtagattag
     2401 tgccatccaa ataacccagc caatgatgaa atacaactgt agcttccaag gggtgttgat
     2461 cgattgccgg gcaggaagcg ccatgaatgg caggtaccat accgccaata aaatagtaag
     2521 ccatgctgta gtttggaatg agcctcggcg caaggaattc tctaaagcga tagcgagatt
     2581 taatgccatc atgatatagg caatggcatg ccaaacccaa gcccattgtt gccaagcatg
     2641 ctgcatggaa ttttgtttca tactggcatt ataagagatt agcaaaagtt atttttgtac
     2701 acaggcttta taaatacaag gtgcgcccaa aaataatggc atataaaagg ataaacccca
     2761 ctcctacaaa aatccacgac caattacgcg gcatcgcaaa cgggggcatg cctgtgtctt
     2821 ttgaacggtc aagcaactgc atcatccatg ccataccaga ggtgcccccc atactggaag
     2881 caaaggatga gaccaataca atgacgattc catatgggtt gaaaagaccg atcaaaatag
     2941 caataccacc ccccactagg tatgcaatta cggagagcat ttgagcgcgc cctactcgct
     3001 ctctaccttc gccaccaatg aaatgatcca tcatctttat tgagattcgt atgacagcat
     3061 aataaaaaac cagccctaaa acagacagcc ccatgcgtat caaccattct ggctgcaccc
     3121 cttgaaatgc accactttca cctgttccca agtcaccgat gcctgctaca ccagagaaaa
     3181 gaaaataccc agatgccatc attaagttga ctgtggcaaa atgccataga aaaaaggtca
     3241 atgaagcatc tgtacgctga ttacgataga aataccctaa tgcaatcaac ccagttagaa
     3301 aactaaccaa aggtccagcc acagccacta cacggtt
//
LOCUS       DAOQJE010000342         3330 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_18581_length_3330_cov_4.799084, whole genome shotgun sequence.
ACCESSION   DAOQJE010000342 DAOQJE010000000
VERSION     DAOQJE010000342.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3330)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3330)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3330
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18581_length_3330_cov_4.799084"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..711
                     /locus_tag="PLT08_09740"
     CDS             <1..711
                     /locus_tag="PLT08_09740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HNE04788.1"
                     /translation="IHANVKPVDTLLVIDSMIGQEALNIAQGFKETIDLTGLILTKMD
                     GDSRGGAAISIRSVTGVPIKFIGTGEKLDALETYDPSRLSSRILGMGDLIGLIEKAEA
                     AYDAETAEKQAKKLMKGDFSLEDWLDQMNQMKKMGPLSQIMEMLPGQMGQAARGIDPS
                     LVEKQFKQSEAIINSMTLKERRDPDILNASRRRRIAAGAGMDVQDVNRLIKQFREAQK
                     LMKTIQKTGGRGLGKMFG"
     gene            854..1195
                     /gene="rpsP"
                     /locus_tag="PLT08_09745"
     CDS             854..1195
                     /gene="rpsP"
                     /locus_tag="PLT08_09745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HNE04789.1"
                     /translation="MVRIRLRRIGLKGQPTYRIVAADKEAPRDGRFLEILGVYNPRTN
                     PATIKLNEDRIYHWMKNGAQPTESAAQVLKTAGTLERFERFKKGEAVETLVAEAAAAE
                     AKRAAPVKTRK"
     gene            1270..1503
                     /locus_tag="PLT08_09750"
     CDS             1270..1503
                     /locus_tag="PLT08_09750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HNE04790.1"
                     /translation="MKELIEYIAKSLVDHPEEVIVTETKGNRPRLGLTVAKEDMGRVI
                     GKGGKVANAIRTLLRVAAERQGKQATLDVTEPS"
     gene            1503..2045
                     /gene="rimM"
                     /locus_tag="PLT08_09755"
     CDS             1503..2045
                     /gene="rimM"
                     /locus_tag="PLT08_09755"
                     /inference="COORDINATES: protein motif:HMM:TIGR02273.1"
                     /note="Essential for efficient processing of 16S rRNA;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HNE04791.1"
                     /translation="MTTEQNSGSPKGESIYLAIGYLRRPHGVQGEVIMDLHTDFPDRI
                     KSGRQVYIGEKYEAFTIGSARVHGNGLLVKLRGFDSPETAGRFRNQWVYVKSTEVPAL
                     PEGQHYKHELIGLTVMTDDDTKLGLLNEVLETGANDVYVIVKEDGKEILLPAIPDVVL
                     NINMADKVMRVHLIDGLLDT"
     gene            2083..3189
                     /gene="dprA"
                     /locus_tag="PLT08_09760"
     CDS             2083..3189
                     /gene="dprA"
                     /locus_tag="PLT08_09760"
                     /inference="COORDINATES: protein motif:HMM:TIGR00732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HNE04792.1"
                     /translation="MDDKKYWIGFNLIKGIGAVRMQGLVAYFGDLESAWNADAPELME
                     AGLGAKLAEKVIGARKQIDLNQVWSKIEAQGIKILTWADEEYPNRLKEIEQPPPVLYI
                     RGEYLMDDLFAVAIVGTRKVTAYGRQVTEEIASFLAANGITVVSGLARGVDAIAHQTA
                     LKAGGRTIGILGSGVDRIYPPEHRGLAEQMVQRGAIISDYAVGTPPDASNFPPRNRII
                     SGLSLAVVVIEAAETSGALITAEFAAEQGREIFAVPGSILAPQSKGTNRLIQKGAQPL
                     LTPADLMQALDLTRMDKQKSARKILPSDETEARVLNTLSSEPLHVDEIRNLANLPIEK
                     ISAALAMMELKGMVRQVGGMNYVAVRDGQSDYMA"
     gene            3233..>3330
                     /locus_tag="PLT08_09765"
     CDS             3233..>3330
                     /locus_tag="PLT08_09765"
                     /inference="COORDINATES: protein motif:HMM:NF012695.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HNE04793.1"
                     /translation="MAGGGGTRLWPLSRKDKPKQLLPLIGQETLFQ"
BASE COUNT          889 a          794 c          872 g          775 t
ORIGIN      
        1 atccatgcca atgtcaagcc ggtggatact ctgctcgtga tcgattccat gatcggtcag
       61 gaagccttga atattgctca aggctttaaa gaaacgattg acctaacggg gctcatcctc
      121 accaaaatgg acggcgactc gcgcggcggt gctgccatct ccatccgctc ggtgacgggg
      181 gtgccaatca aatttatcgg taccggtgaa aaactcgatg cccttgaaac ctatgatcca
      241 tcacgcctat cttcccgcat tttgggcatg ggcgatctca tcggtttgat cgaaaaagcc
      301 gaagccgctt atgatgccga gacggctgaa aagcaagcca agaaattaat gaaaggcgat
      361 ttctcgctgg aagattggct tgaccaaatg aaccaaatga aaaagatggg tccgctttcg
      421 cagattatgg aaatgctgcc cgggcaaatg gggcaggctg cacgcggaat cgacccctcg
      481 ctggtcgaaa aacagtttaa acaatctgaa gccatcatca attcgatgac tctgaaagaa
      541 cgccgcgacc ccgatatttt gaacgcctcc cgtcgccgtc gcatcgctgc cggtgctggc
      601 atggatgtgc aggatgtaaa ccgcctcatc aagcaattcc gtgaagcgca aaagctcatg
      661 aagaccattc aaaaaacagg cggtagggga ttagggaaaa tgtttggcta gattgtctct
      721 cagaccccaa ggatattggg gtctttttat tgttttctct ggtaaaatac agcccgcgat
      781 ttcagcccgc acatccctct cgtctctagt gcctgtctga aacgtaatca aaatctaaag
      841 gagcaagttt aagatggttc gtattcgttt acgtcgtatt ggtctcaagg gccagcccac
      901 gtaccgcatc gtcgctgctg ataaagaagc cccccgcgat ggtcgctttt tggaaatttt
      961 gggtgtgtac aatccccgta ccaaccctgc cacgatcaag ttgaatgaag atcgcatcta
     1021 tcactggatg aagaacggcg ctcagcccac cgaatccgct gcccaggtgc tcaaaactgc
     1081 tggcaccctc gagcgtttcg aacgcttcaa gaagggcgaa gctgttgaaa ccctcgttgc
     1141 agaagccgcg gcagctgaag ccaagcgcgc tgcacccgtc aagacccgca agtaaatcgc
     1201 aggactcatc attacggatt acgcaatttc gaaagcgtaa tccgtaatgt gtaaatgagg
     1261 aaatttaaaa tgaaagaact gatcgaatat attgcgaaat cattagtgga tcaccccgaa
     1321 gaggtcattg tgaccgaaac caagggcaac cgtccgcgcc ttgggctgac cgtagccaaa
     1381 gaagatatgg ggcgcgtgat cggcaagggt ggcaaagtgg caaatgccat tcgcaccctc
     1441 ctgcgtgtgg cggctgaacg ccagggaaaa caagccacct tggatgtgac ggaaccctcc
     1501 taatgacgac cgaacagaat tcaggctcgc caaaaggcga gtctatttat ttagcaattg
     1561 ggtatctgcg ccgaccgcat ggggtgcaag gcgaagttat tatggatttg catacagact
     1621 tcccagaccg catcaaatca ggacgccaag tttacattgg cgagaaatac gaagccttta
     1681 ctattggctc tgcccgtgtg catggaaatg ggctactggt aaaactgcgc gggtttgact
     1741 ctcctgaaac ggctgggcgt ttccgcaatc agtgggtgta tgttaaatcg acggaagtcc
     1801 ctgcactgcc tgaaggtcag cattacaagc acgagttgat cggtctcacc gtgatgacgg
     1861 atgacgatac aaaacttggt ctcttgaatg aggtccttga aactggcgca aatgatgtgt
     1921 atgtcattgt caaagaagac ggcaaagaga ttcttttgcc tgcaattcca gatgtagttc
     1981 tcaatatcaa catggctgat aaggttatgc gagttcacct tatagatggt ctgctcgaca
     2041 cctaaaacaa aaccaagcgg ggagtgttag aatcgcgttt caatggacga taaaaagtat
     2101 tggattggct tcaatctcat caaaggcata ggcgctgtcc gcatgcaggg gctggtcgcc
     2161 tattttggcg accttgagtc tgcttggaat gcagatgccc ctgaattgat ggaagccggt
     2221 ctgggcgcaa aattggcaga gaaagtgatc ggggcgagaa agcaaattga cctgaatcaa
     2281 gtttggtcaa agatcgaagc gcaaggcatc aaaatcctca cgtgggcaga tgaagaatat
     2341 ccgaatcgcc tcaaggaaat cgagcaaccg ccgcctgtcc tttacatccg cggcgaatat
     2401 ttgatggatg acttgtttgc ggttgccatt gttggtacgc gcaaggtaac agcatatggc
     2461 aggcaagtga cagaagagat tgcctctttc ctcgccgcaa atggcatcac cgtcgtgagc
     2521 ggactcgccc gtggagtgga tgccatcgcg catcagaccg cgctcaaagc cggcgggcga
     2581 acgattggta ttcttggttc tggagtagat cgaatttatc cgccagaaca tcgtgggctt
     2641 gccgaacaga tggtgcagcg cggagcgatc atcagtgact atgctgttgg caccccgcca
     2701 gacgcttcaa actttccgcc aagaaataga atcatttcag gtttatcatt ggctgtggtg
     2761 gtgatcgaag ctgctgaaac cagtggcgct ctaatcactg ctgaatttgc tgcagaacaa
     2821 gggcgtgaga tctttgccgt gccgggtagc attttggcgc cgcaaagcaa aggcacgaat
     2881 cgcctgatcc aaaaaggagc acagcctttg ctcactccag cagacttgat gcaagcctta
     2941 gacttaaccc gcatggacaa acaaaaatcg gctcgcaaga ttttgccttc cgatgagact
     3001 gaagcacgag tcttgaatac tttgagtagt gaacctctac acgtggatga gattcgaaat
     3061 ttggcaaacc tgccaattga gaaaatttca gccgcattag ctatgatgga attgaaaggt
     3121 atggttcggc aagtcggtgg tatgaattat gtagctgtcc gcgatgggca gtctgattac
     3181 atggcttagg agaaaagcta tgtcagattt atcacatgcc tatgcggtga ttatggcagg
     3241 cggtggtggg acgcgtttgt ggcctctttc ccgcaaagat aaacccaaac aacttttacc
     3301 tttgattggg caggaaactc tttttcaaag
//
LOCUS       DAOQJE010000343         3323 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_18644_length_3323_cov_4.970318, whole genome shotgun sequence.
ACCESSION   DAOQJE010000343 DAOQJE010000000
VERSION     DAOQJE010000343.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3323)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3323)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3323
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18644_length_3323_cov_4.970318"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1362)
                     /locus_tag="PLT08_09770"
     CDS             complement(<1..1362)
                     /locus_tag="PLT08_09770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE04794.1"
                     /translation="MYFALKDRSEIGKALEEKRNNYEFFLKDSGRQNLYNNLYREFYQ
                     AKYHHGNIYATGENGEFLNININEFRTGLQHIKSLILSQKITLSPAATNTDTDALSQS
                     LLGKNVMAHYAKKLKVDLLMDTSIDLANLYADMYALTEWDVTQGAVYIEDDLGQKVYE
                     GDPKITLFPPHMVAIDPMNPVSDWHIIMVKGNKYELAVLYPEYASEIEKQQMQPKYVF
                     DAQINLSKYTGDTICYFKFFHKDCPLVPGGRQVTYITDTIVLEDKTLEGLPYPVTKFQ
                     VSAVEDSPFGYTAAFDAIQISQANNKVYSTIVTNESKFGIKPILFPKGCDIEPIDVAG
                     GAIGVEYDPTAGVPSALDLLATSEQTFGLGSMLTSKLDITFGINDVTKGQAPKDIKSG
                     SGLALLQSMSVQFNSTLQKNFVIFLEELFTKFLYLLQKKVTNDRLVEIVGKDQQMYTK
                     QWSG"
     gene            complement(1381..2820)
                     /locus_tag="PLT08_09775"
     CDS             complement(1381..2820)
                     /locus_tag="PLT08_09775"
                     /inference="COORDINATES: protein motif:HMM:NF015211.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase family protein"
                     /protein_id="HNE04795.1"
                     /translation="MQLTKQQIQDELWKRGEISWKLHEGQVKINRTVNASKSFLAQNM
                     CARQFGKTVYNLWEATNDCLNNPGTVVKYGAAFYTDLEKYIFPTAYEFFADCPKEIRP
                     EIIESKKLIYFPHKRATIDFFGCDRNPDGARGPKVKKVILDEAGYINKLRYIYYDVIL
                     PMFTHTIDLNPKCIMSGTPPESPDHPFTSFFMPINKKDGSFIKLTIDDNPLLSQEHKD
                     RLIAEYFRGVESEGHRKVQWQKMRRELYCDILVDQERAIIPEFHHGIVQKFERDKYFP
                     HRHKYVGMDLGTKHKTVALYAVYDFLNARLHVMDECQMFGPTMTTKNLADLLKSKERD
                     VFGNYKPFKRVSDNNNLLLLQDLNLQYNLHFSPVVKRSNDKLVRRPLMSMVNKIRVWM
                     GEGRILVDPKCKELIGCLESGIWDNHREGFEESEVYGHYDALAALIYMLQVVDEKTNP
                     TPMLFGKNPENTYIFPKEKKYNKFFKMFR"
     gene            complement(2811..>3323)
                     /locus_tag="PLT08_09780"
     CDS             complement(2811..>3323)
                     /locus_tag="PLT08_09780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE04796.1"
                     /translation="KSALVKRAIGQTITKEYQSLSKSGEVVDLIEKVEIPGDVKAQLA
                     YLQAKDKDGGWDNKSNTVNVQFNQVMQGISTNDLLLTLRSAPEQLSTGGEGGGVLEVS
                     GVREEKLPITPNPISPTYDANYDNFHDAKGKKKTSRSALDTYFDPNNGRLDEPVEGML
                     DNVGEDEECS"
BASE COUNT          934 a          869 c          539 g          981 t
ORIGIN      
        1 cccagaccat tgcttggtat acatctgctg atctttacca acaatttcca ccaaacgatc
       61 attggttact ttcttttgca acaagtaaag aaatttcgta aatagttctt ccaaaaatat
      121 cacaaagttt ttctgcaacg ttgagttaaa ttgcactgac atcgattgaa gcagtgccaa
      181 accagatccc gacttaatat ctttcggtgc ttgccccttg gttacatcat taatcccaaa
      241 cgtaatatcc aactttgatg taagcattga ccccaaacca aaggtctgct cacttgttgc
      301 aagcaagtcc aatgcacttg gaaccccagc cgttggatca tattcgacac caatcgctcc
      361 accagccacg tcaatcggct caatgtcaca acctttagga aataaaatcg gcttaatacc
      421 aaacttcgac tcattggtaa caatggttga atatacctta ttattcgcct gagaaatttg
      481 tatcgcatca aaagccgctg tatacccaaa cggagagtct tctactgccg acacctgaaa
      541 cttagttaca ggatacggta aaccttccaa tgttttatct tcaagaacta tagtgtctgt
      601 aatgtaagta acttgtcttc ctccaggaac caaaggacag tctttgtgaa aaaatttgaa
      661 gtaacaaatc gtatctccag tatatttaga aagattgatc tgtgcatcaa aaacatactt
      721 tggttgcatt tgctgtttct ctatctctga agcatattca ggataaagaa cagcaagctc
      781 atacttgtta ccctttacca ttatgatgtg ccagtctgac accggattca tcgggtcaat
      841 ggccaccatg tgcggtggga atagcgtaat cttcggatct ccctcataca ccttctgccc
      901 caaatcatcc tcaatataca cagccccttg cgtaacgtcc cattctgtca gcgcgtacat
      961 atccgcatac aaattggcaa gatcaatgga cgtatccatg agtaaatcta cttttaactt
     1021 cttggcgtaa tgtgccatca cgttcttacc aagtagcgac tgagaaagcg catccgtatc
     1081 tgtatttgtt gccgctggag aaagtgtgat cttctgagaa agtatcaaag acttaatgtg
     1141 ctgcaagcct gtacgaaatt cattgatatt gatatttaaa aactcaccat tctcacccgt
     1201 tgcatagata ttgccatggt gatattttgc ttggtaaaac tctcgatata aattgttata
     1261 taaattctga cgacctgagt cttttaaaaa aaactcataa ttatttctct tttcttcaag
     1321 agctttccca atctcagacc tatcttttaa cgcaaagtac attgctttta cgatactccc
     1381 tcatcgaaac attttaaaga atttattgta ttttttttct tttgggaaaa tgtaagtatt
     1441 ttcaggattt tttccaaaca acatcggcgt aggattggtc ttctcatcca ctacctgaag
     1501 catataaatc aaagcagcaa gagcgtcata gtgcccgtaa acctccgact cctcaaaccc
     1561 ctcccgatga ttgtcccaaa tccctgactc caagcaccca ataagctcct tacacttcgg
     1621 gtccaccagt atacgccctt ctcccatcca tacccgaatc ttattcacca tactcatcag
     1681 tggtctacga accaatttat catttgatct cttaaccaca ggactaaaat gtaagttata
     1741 ctgcaaattt aaatcctgaa gtaacaacaa attgttattg tccgatactc gcttgaaagg
     1801 cttgtaattc ccaaacacat ctctttcctt gctttttaaa agatccgcaa ggttcttcgt
     1861 cgtcatcgtt ggaccaaaca tctggcattc atccatcaca tgaagtctag cattcaaaaa
     1921 atcatacacc gcatataacg ctaccgtctt atgctttgtc cctaagtcca tccccacata
     1981 cttgtgccga tgcggaaaat acttgtccct ctcaaacttc tggacaattc catggtgaaa
     2041 ttctggaata atagcgcgtt cctgatcaac gagaatgtcg caataaagtt cccgtctcat
     2101 cttctgccac tgcaccttgc gatgcccctc actctcaaca ccccgaaaat actccgcaat
     2161 caacctgtcc ttatgctcct gagaaagcag tggattgtca tcaatggtca acttgataaa
     2221 actcccatcc ttcttgttaa tcggcatgaa aaaactcgtg aacggatgat ccggcgactc
     2281 cggcggcgtc ccactcataa tgcacttagg attcaaatca attgtgtgcg taaacatcgg
     2341 caaaatcaca tcatagtaaa tatacctaag cttgttaata taaccagcct catccagtat
     2401 caccttcttc accttcggac cacgagcccc gtccggattc ctgtcacatc caaaaaaatc
     2461 aatcgtcgcc cgcttgtgcg gaaagtaaat caattttttc gattcgataa tctccggacg
     2521 tatctccttt ggacaatccg caaaaaactc atacgccgtc ggaaatatat acttctcaag
     2581 gtccgtataa aatgccgccc catacttcac cacagtgcct gggttattca gacaatcatt
     2641 cgtcgcttcc caaaggttgt aaacagtctt cccaaactgc cgcgcacaca tgttctgcgc
     2701 caaaaaagat ttacttgcgt tcacagtccg attaatctta acctgcccct catgcaactt
     2761 ccatgaaatc tcaccacgct tccataattc atcctgtatc tgctgcttgg ttaactgcac
     2821 tcctcatcct ctccaacatt atccaacata ccctccacag gctcgtccaa tcttccatta
     2881 ttagggtcaa aataagtgtc caatgcagaa cgactcgtct tcttttttcc tttagcgtca
     2941 tgaaaattgt cgtagttagc atcgtaagtc ggggagatag ggtttggggt gatgggtaat
     3001 ttttcttctc tcactccact aacctcaagc acacctcccc cctccccccc tgtgctcaac
     3061 tgctcaggtg cggagcgtaa cgtcaacaat aaatcattgg tgctaatgcc ctgcattact
     3121 tggttaaact gtacattaac tgtattgctt ttattgtccc agccaccgtc tttgtctttc
     3181 gcctgcagat acgcgagctg tgccttcacg tcgccaggta tttcaacctt ttcgatcaaa
     3241 tcaaccactt cgcccgactt actcaaagac tggtattctt tggttatggt ttgcccaatc
     3301 gcacgtttca ccaaggcaga ttt
//
LOCUS       DAOQJE010000344         3316 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_18729_length_3316_cov_2.204845, whole genome shotgun sequence.
ACCESSION   DAOQJE010000344 DAOQJE010000000
VERSION     DAOQJE010000344.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3316)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3316)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3316
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18729_length_3316_cov_2.204845"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            251..913
                     /locus_tag="PLT08_09785"
     CDS             251..913
                     /locus_tag="PLT08_09785"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HNE04797.1"
                     /translation="MTKIIQVSILDDHQTILDGYRFRLDSSTKVKIVATMTFGSDVED
                     TLKKHPTDVLVLDINVPTSQENSNPYPILYLIPSLLQKYPNLAILVISMHTDRSLIRS
                     VMEAGASGYILKDDKIMLMDIENVIISIAAGEISLSQEIDSILLQPNMNEVVSLSQRQ
                     KEVLSLSLAFPDDTTAELAKKMSIANSTVRNLLSGAYIKLGVRTRVAAIAKARKLGIL
                     TA"
     gene            1189..>3316
                     /locus_tag="PLT08_09790"
     CDS             1189..>3316
                     /locus_tag="PLT08_09790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE04798.1"
                     /translation="MTFQNSSFRKILEHSLPWLVLGILLFYTYVKFFQHPYGFSWGSD
                     GKVFAVFVDQPESLSVGDQLVQVGTLKWEEFQNDLRVTLFDGFNVGEAVPIIVERNGE
                     RLTVLWKLPGVNVGEVWEQLASEWPIAYFFWLAGTLTLLFLRPKDERWLLLSAFNFLT
                     SIWLAVGGGASGYHIFYSAIILRVTVWLCIPVYLHFHWVFPRPLGKLPALLVWGIYTI
                     SFALALAQWFQWLPADSYLLGFLIAIVGSIALLIAHAIKQSDVRRELRLLLITMFFVL
                     GPLIILGFVQSYPGTISRLGSLALLPFPLLPFAYLYAAYRRQLGSSEMRINRLISIYF
                     FIIILFSVCLPLLAVLYNIFATVENILLIGILTTIFTSLISIWVFPNFQSFVERRLLG
                     ITLSGQDLPQIYSARITTSTSLTNLLKLLEDEIFPSLLVNQYAFVRFAGGSGEILLSK
                     NLDKDQLQKETLEHLFTVLNTESSHPNIGGEQTLDWIRLILPLKLDAELMGAWLLGRR
                     DPDDLYPQAELSILQSMANQTTIALSNILHSNRLLNLYANDIERYEKSRVQLALELHD
                     NVLTQLAILRLNTDDSTLSSNFENAYKQVSTYLREIVSDLRPPMLVYGLQMAIKSLED
                     KITIRAQGVTKVFINFQNENNIRYPENVERHIFRIIQQACDNAIQHGKATEIHIHANM
                     DMQSVWILVEDNGIGFDSNGKLNLETL"
BASE COUNT          897 a          631 c          669 g         1119 t
ORIGIN      
        1 aaagccctct atgagtcgac tcgtaagttt tatattggaa caggctacca agccgaagca
       61 accatcaagg atttttacac caaaggcgat gaccttgtta tttatgtgaa acacctttag
      121 gagttttggc ttgcgaatac cgtctaaaat gttcctttaa gctcatatag gcacatgtac
      181 tttattaggc taaactttaa gcgccgttta tttttttctg ataacctatt attaagacag
      241 gagggttccc gtgacaaaaa taatccaggt cagtattctc gacgatcatc aaaccatttt
      301 agacggatat cgatttcgtt tggatagtag tacgaaagta aaaatagtag caacgatgac
      361 gtttggaagt gacgtagagg acactttaaa aaaacaccct actgatgttt tagtgttgga
      421 cataaatgtt cctacttcac aagaaaattc aaacccctat ccaattctgt atctaatccc
      481 aagtttgctt caaaaatatc caaatctggc tattcttgta ataagtatgc atactgatcg
      541 tagcttgatt cgttctgtaa tggaggcggg cgcaagtggc tatatactca aagatgataa
      601 gattatgcta atggatattg agaatgtaat catttcgatt gctgccggag aaatttcact
      661 gagccaagaa atagattcta ttttgctcca gccaaatatg aatgaagttg tgtctctttc
      721 acaacgacaa aaagaagtcc tttctcttag tttggcgttt cctgatgata caactgctga
      781 gcttgcaaaa aagatgtcta ttgcaaattc gacagttcgt aacctgcttt caggggctta
      841 cataaagtta ggagttcgga ctagagttgc ggcgattgca aaagctcgta aattaggtat
      901 tcttactgct tagttatttt tgtttgaggg ttctgtactt aagaatgtaa gtttcttatc
      961 aatcacaaat tgattttcat ctctagtaat ttctattact aattctccag aatactataa
     1021 attaccaatt ctattaagat acagaaaatg ttttctgtat cttttgacat gctatacttg
     1081 ctttatatac tcgtgttgtc ttatgtaact cttttgatta ttcaatttgt acgctagctt
     1141 gtcattagtc ttattgagaa tctctgcttt tgcgaaggga catcattgat gacatttcaa
     1201 aacagctcat ttagaaagat tcttgaacac tccttgcctt ggctagttct tggcattctt
     1261 ttgttttata cgtatgttaa gtttttccag catccttatg gatttagctg gggttcagat
     1321 ggcaaggtgt ttgcagtatt tgtagaccag ccggaatcct tgagcgttgg agaccaattg
     1381 gtgcaggtgg gaacccttaa atgggaagaa tttcagaatg acttacgagt aactttattc
     1441 gatgggttta atgtgggcga agcggttcct attattgttg aacgtaatgg agaacgcctc
     1501 actgtgttat ggaaactgcc aggtgttaat gtaggagagg tatgggagca attggctagt
     1561 gaatggccaa ttgcttattt cttttggctg gcagggactc tgaccttatt attcctgcgt
     1621 ccgaaggatg agcgttggtt attgctttcg gcttttaact tcttaacttc catttggttg
     1681 gcagtcgggg gaggagcatc aggctatcac attttttaca gtgcaatcat cctgcgtgtc
     1741 acggtttggt tatgtattcc cgtatattta cattttcact gggtgtttcc acgtccattg
     1801 ggtaaactcc cagcgctgct tgtctggggt atctatacca tctcatttgc ccttgccctt
     1861 gctcagtggt tccaatggct gccagcagat tcttacctgc ttggttttct gattgccatt
     1921 gttggaagta tcgccttgct cattgctcac gcgatcaaac agtcagatgt gcgccgtgaa
     1981 cttcggctgc ttttaatcac aatgtttttt gtcttaggtc ctttgattat tttggggttt
     2041 gttcagtctt acccaggtac gatttcgcgc ctggggtctt tggcattgtt gcctttccct
     2101 ttgctgccat ttgcttattt gtatgctgcc tatcgccgcc aactaggtag ttcagaaatg
     2161 cggataaacc gtctgatatc gatttacttc tttatcataa ttctttttag cgtttgtctt
     2221 ccgttactgg cagttttata taatattttt gcaactgttg aaaatatatt acttataggt
     2281 attcttacaa caatctttac atctctcatc tctatttggg tgtttcctaa ctttcaaagt
     2341 tttgttgagc gtcgcttgct tgggattacc ctgtctggac aagacttacc tcagatttat
     2401 tctgctcgta ttaccaccag cacctctctc actaatttgt taaaactctt ggaagacgaa
     2461 attttcccca gtttgttggt gaaccaatat gcatttgtac gatttgcagg tggttcggga
     2521 gaaatcctcc tttctaaaaa tctggataaa gaccaacttc agaaagaaac cctcgaacac
     2581 ctatttactg tattgaatac cgaaagctct catccaaata taggaggaga gcaaactctt
     2641 gattggattc gattgatcct gccattaaag ttggatgcgg aattgatggg agcctggctg
     2701 ttggggcgcc gcgatccaga tgatttgtat ccacaggcag aactttcaat tctgcaatca
     2761 atggcaaatc agactacgat tgcattaagc aacatacttc attcaaaccg tctacttaat
     2821 ttgtatgcca atgatattga aagatatgaa aaaagtcgtg tgcaactggc tttggaactg
     2881 catgataatg tacttacaca actggcaatt cttcgtctaa acacagatga ctctaccctt
     2941 tcttcaaatt ttgaaaatgc ttataaacaa gtcagcacct atctgcgtga aattgtctct
     3001 gacctgcgtc cgcccatgtt ggtttatgga ttacagatgg ctattaaatc tcttgaagat
     3061 aaaatcacaa tccgtgccca gggtgttacc aaggttttta taaatttcca gaatgaaaat
     3121 aacattcgtt acccagagaa tgtcgaacgt catatattta ggataataca acaggcatgt
     3181 gataatgcga tacagcatgg aaaagcaact gaaattcata ttcatgcgaa tatggatatg
     3241 caaagtgtat ggatattggt agaggataat ggtattggtt tcgattctaa tggaaaactc
     3301 aatcttgaaa ctttgc
//
LOCUS       DAOQJE010000345         3312 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_18771_length_3312_cov_5.239484, whole genome shotgun sequence.
ACCESSION   DAOQJE010000345 DAOQJE010000000
VERSION     DAOQJE010000345.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3312)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3312)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3312
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18771_length_3312_cov_5.239484"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..774
                     /gene="mreB"
                     /locus_tag="PLT08_09795"
     CDS             <1..774
                     /gene="mreB"
                     /locus_tag="PLT08_09795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreB"
                     /protein_id="HNE04799.1"
                     /translation="HEQSVVPLPRPRVIVGLPTGVTEVEKRAVYDAVMASGARQAMLI
                     EEPIAAAIGAGLPIGEVRGSMVVDIGGGTTEVAVMSTGGVVASRSLRVAGDEMDQDVV
                     QYLRNKYNLLVGTGIAEQIKWQIGSAYPLQPEKTMEVRGRNLVTGLPETVEISSVEIR
                     EALSGSVQVIIDTVRDALDEIPPEIVSDLMDIGICLAGGGALLQGLAERLTDELKLRV
                     WVAEDPLTCVARGAATVFEDLESLSRYLIGLERGSTRHV"
     gene            811..1653
                     /gene="mreC"
                     /locus_tag="PLT08_09800"
     CDS             811..1653
                     /gene="mreC"
                     /locus_tag="PLT08_09800"
                     /inference="COORDINATES: protein motif:HMM:TIGR00219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HNE04800.1"
                     /translation="MNSRSLQTTIIFLVIGGILVLAFSGALGSASRGFTSVLVDVQTW
                     ISSRFLGFQDFVTAPRDIVTLRTRNAELEAQVSQLQAQLIELQQRVNETEILAALVDF
                     SRSNPESTYKAASVIGRDPSPFLHYIIINRGSNDDIRRGMPVVTNQGLVGRVDAVIAD
                     AARVQLITDPSSSVNVYLQNADTSAVLYGSVTGDVSLDLISQNASVEAGDLILTSGLG
                     GGYPADLLIGQVVTVRSLEFELFQQATVQPAVDFSRLEIVLVITNFRPVDIAPLEPVV
                     TPSP"
     gene            1660..2154
                     /locus_tag="PLT08_09805"
     CDS             1660..2154
                     /locus_tag="PLT08_09805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE04801.1"
                     /translation="MRNLIAFPLIGLAVILQSAVVSQVSLLSGYADLPLVLLAAWALQ
                     DRVDSSWHWGVLTCLFAGFISGLPISVVIAGYLGIILFARILQRRVWQAPLLAMFSVV
                     FLGTLFFHILSFVVLRFSGTPFAFGDVIGLITLPSLLLNMLISIPIYAFMRDVSYWVY
                     PVKE"
     gene            2157..>3312
                     /locus_tag="PLT08_09810"
     CDS             2157..>3312
                     /locus_tag="PLT08_09810"
                     /inference="COORDINATES: protein motif:HMM:NF015662.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HNE04802.1"
                     /translation="MSSSAESRPVRFEGWRLGIIYVVVSLTLIGFVIRLVNLQIFNVD
                     QYEARAVENYAKPISVPAPRGIIYDRNGYILARNIAAYNVVITPASLPAEDSDIQRIY
                     RELSQLIDVPVGNFVANDPESNEIFLSVPGGLLAETYLEEAKLFAACVPGPSIGQLVA
                     LQDTLAPYSAVKIKCNVSEEIARIVEEKKMDWPGVSVEVEPIRDYPTGSLTSHLIGFL
                     GPIPASQEEELKALNFDPNRDKVGYAGVETSLQEILAGRNGLRVVQVDVAGQEIRNLE
                     PPIPAVPGYNVYLTIDTRLQAAAQATLEEEIRYWNTYFGQIRISSGVIIAINPKTGEI
                     LAMVSYPNYENNRFSRLIPAYYYDQLSQDPRRPLLNNAISAEYPPGSTFKL"
BASE COUNT          788 a          696 c          823 g         1005 t
ORIGIN      
        1 catgaacaga gtgttgtgcc tttgccacgt ccgcgtgtga tcgttggttt acctaccggt
       61 gtgaccgaag tggaaaagcg cgctgtgtat gatgcagtaa tggcatcagg cgcgcgccaa
      121 gctatgttga ttgaagaacc aattgcggcg gcgatcggag ccggtcttcc aattggagaa
      181 gtccgcggtt ctatggtcgt tgacattggt ggcggcacaa ctgaagtcgc agtcatgtct
      241 acgggtggtg tagtggcgtc acgttcattg cgcgtggctg gtgatgaaat ggatcaagat
      301 gttgtgcagt atttgcgcaa caaatacaat ttattagtgg ggacggggat tgctgaacag
      361 atcaaatggc aaattgggtc ggcatatcca ttgcaacctg aaaaaaccat ggaagtgcgc
      421 ggcagaaatt tagtgactgg tttacctgaa acggttgaaa tttcttctgt tgaaattcgc
      481 gaagcgctct ctggttcggt gcaagtgatc attgataccg ttcgcgatgc cttggatgaa
      541 attccgcccg aaatcgtttc agacttaatg gatattggta tttgtttagc cggcggtggg
      601 gcactcttgc aaggcttggc agaacgtcta accgatgaac tgaaattacg tgtatgggtt
      661 gctgaagacc cacttacttg tgtagcacgt ggtgcagcca ctgtattcga agaccttgag
      721 agcctttctc gctatttgat cggtctcgaa cgtggcagta cgcgtcacgt ataggattaa
      781 gtagacagcc tttcgtgggc tgaataaaaa atgaactctc gttccttaca aacgacaatt
      841 atttttctgg tgattggggg gattctagtg cttgccttta gcggcgcatt aggttcagct
      901 tccagaggat ttacttctgt gcttgttgat gtccaaacgt ggatatccag ccgttttctt
      961 ggctttcaag attttgtcac tgcgccacgc gatatcgtca cattacgtac gcgtaatgca
     1021 gagttggaag cgcaagtttc tcaattgcaa gcacaactca ttgaattgca acaacgtgta
     1081 aatgagaccg aaattcttgc ggctcttgtt gatttttccc gttcaaaccc tgaaagtaca
     1141 tataaagctg catcggtcat cggtcgtgac ccaagcccat ttttgcatta catcatcatc
     1201 aaccgcggtt cgaatgatga tattcggcgt ggtatgcccg ttgtgaccaa tcaaggtctc
     1261 gtggggcgtg ttgatgcggt cattgcagat gctgcccgcg ttcagttaat cacagatcca
     1321 tcttcttcgg tcaatgtata tttgcaaaat gcagatacaa gcgctgtttt gtatggttcg
     1381 gtcactggcg atgtttctct tgacctaatc tctcaaaatg cctctgtaga agcaggcgat
     1441 ttgattctca cctcagggtt gggtggagga tacccagcag acctcctgat cggacaagtc
     1501 gtcacggttc gctctttgga atttgagctg tttcaacaag cgacagttca acccgccgtt
     1561 gacttttccc gcttggaaat cgtccttgtc atcaccaact tccgcccagt ggatattgct
     1621 ccacttgagc cagtagttac accttccccg taatttttca tgcgtaattt aatcgctttt
     1681 ccgttaatag gattggcagt cattctgcaa tctgcggttg tcagccaagt aagtctactc
     1741 tcagggtacg ctgacctgcc gctcgtattg ttggcggctt gggcactaca agaccgtgta
     1801 gattcttctt ggcattgggg cgtcttaacc tgtctctttg ccggattcat ctcaggttta
     1861 cctatttctg ttgtaattgc tgggtacctc ggaattatat tgtttgcccg cattttacaa
     1921 agacgtgtat ggcaggcgcc tttgctggca atgttcagtg tggttttcct agggacattg
     1981 ttttttcata ttttgtcttt tgttgtgctt cggttttcag gtacaccttt tgcttttggt
     2041 gatgtgatag gattaatcac cttgcctagt ttattgttga acatgttgat ctcgatccca
     2101 atatatgcat tcatgcgtga tgtgtcttat tgggtgtatc ctgtaaagga gtaagaatga
     2161 gttctagtgc agagtcccgt cctgttcggt ttgagggatg gcgattggga ataatctatg
     2221 tagttgtttc tcttacattg attggatttg tgatccgttt agtgaatcta caaatattca
     2281 atgttgacca gtacgaggcg agagcggtgg aaaattatgc caaacctatt agtgttcctg
     2341 ctccacgcgg aattatttat gaccgtaatg gatacatctt ggctcgtaac attgctgcgt
     2401 acaatgtagt aatcacgcct gccagtctac ctgctgaaga ctcagatatt caacgcattt
     2461 atcgtgaact atctcaattg attgatgtac ctgtaggcaa ttttgttgca aatgaccctg
     2521 aaagtaacga aatattttta tctgtccctg gcggtttgtt agcagagaca taccttgaag
     2581 aagcaaagtt gtttgctgcc tgtgtgccgg gaccaagtat tggtcagtta gtggcattgc
     2641 aagatacgtt ggctccatat agtgcagtaa agatcaaatg taatgtttct gaagaaatag
     2701 ctcgcatcgt ggaagagaaa aagatggact ggcccggcgt gagcgtggaa gtggagccga
     2761 ttcgtgatta tccaactggc tcgctaactt cacatctaat tggcttttta gggccaatcc
     2821 ccgcgtcaca agaagaagag ttgaaggcat taaactttga cccaaatcgc gataaagttg
     2881 ggtatgcagg cgtagagact tcgttacaag aaatcttggc gggcaggaat ggtttacgtg
     2941 ttgtacaagt agatgtggca ggtcaggaaa tccggaattt ggagccgccc attccggctg
     3001 ttccgggcta taacgtgtac ttaaccattg atactcgttt acaagcggct gctcaagcta
     3061 ccttggaaga agaaattcgc tattggaata catacttcgg tcaaattcgt atttctagtg
     3121 gtgtgatcat tgctataaat cctaaaacag gcgagattct tgccatggtt tcgtatccga
     3181 attacgagaa caatcgtttt agccgcttaa tcccagcata ctattacgat caattgagtc
     3241 aggatccgcg aagaccgttg ctgaataatg caatttcggc tgaataccct cctggttcga
     3301 ccttcaagct tt
//
LOCUS       DAOQJE010000346         3309 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_18793_length_3309_cov_7.517517, whole genome shotgun sequence.
ACCESSION   DAOQJE010000346 DAOQJE010000000
VERSION     DAOQJE010000346.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3309)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3309)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3309
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18793_length_3309_cov_7.517517"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..592
                     /locus_tag="PLT08_09815"
     CDS             <1..592
                     /locus_tag="PLT08_09815"
                     /inference="COORDINATES: protein motif:HMM:NF024376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PhnD/SsuA/transferrin family substrate-binding
                     protein"
                     /protein_id="HNE04803.1"
                     /translation="FYGAQILINRENVFTAYYDEERADNTTDALSALKQFADKKPCWS
                     DETSPSGYVVPLGLLNQYQVQTRAGAFLEGQPNVVRGVYAADICDFGATFVDARNSPI
                     LEADYPDVMDKVRVAWRIPEIIPYENISMAASLPFEMRRSVQRSLIDFMVTADGKAVM
                     QTIYGFDEMLPIEDASYAEFIKYVNASGLNLLDLIE"
     gene            597..1244
                     /locus_tag="PLT08_09820"
     CDS             597..1244
                     /locus_tag="PLT08_09820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ElyC/SanA/YdcF family protein"
                     /protein_id="HNE04804.1"
                     /translation="MFKRVIRFLWRTLLVLSALTAIGLFMPRWIMALFAAPRTFTVED
                     VPATNVVIVFGAGLLRDGSAGPVLSDRMLTAVNLYHAGKVEKILVSGDNRFIEYNEPE
                     AGRQYALEHGVPDEDIVLDYAGRRTYDTCYRAKHIFGVNEAILVTQDFHMPRALALCN
                     WFGLNAVGVESNNRYFLKRSQVWWNFRETFAVFQAAWDLMVTKPLPVMGEPEPIN"
     gene            1277..1852
                     /locus_tag="PLT08_09825"
     CDS             1277..1852
                     /locus_tag="PLT08_09825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDIG domain-containing protein"
                     /protein_id="HNE04805.1"
                     /translation="MNREEALALVREYVKSESLVRHMLSVEAAMRFYAEKFGEDVESW
                     GLVGLLHDFDWEIHPTLEEHPQAGAPMLRERGVPEDIIQDILSHADHLNMPRDTMRRK
                     AICACDEVTGLITAVALVRPSRSLYDLEASSVKKKWKDKAFAAGTNREEMEHAAKEFG
                     IDVWEHVGNVIVAMRKIAPELDLVGNIPNPS"
     gene            complement(1849..2235)
                     /locus_tag="PLT08_09830"
     CDS             complement(1849..2235)
                     /locus_tag="PLT08_09830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE04806.1"
                     /translation="MEYTIQLDQANGLILVHAAGDWESDKDNLMVQQIMQTVTESGLR
                     KILLDIRELKFDLSMAHIFERAVAMRDTRMKQDKVSSRVALVYDAQNQKLEADMRFFE
                     TASQNRSIPYRVFSDIEEARLWLLKA"
     gene            complement(2311..>3309)
                     /locus_tag="PLT08_09835"
     CDS             complement(2311..>3309)
                     /locus_tag="PLT08_09835"
                     /inference="COORDINATES: protein motif:HMM:NF015922.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase C-terminal beta-propeller
                     domain-containing protein"
                     /protein_id="HNE04807.1"
                     /translation="PKHSGNDAPRFLVKASTTDTVYFVSKFGKAAAVAAHLIPEASKL
                     SQGVPWHRVSPLSDNDALAAVFALPASKSALGEEVCVLTATRFGMIKKSLVTELPGPS
                     SQTFVLVKVNEGDKLNSVGITDGKKDILLVTASGMAIRFKEEDVRPMGLVAAGVNGMK
                     LEDKDEVVGMEILPADGDIFLLTNEGKAKRVDQKDFPVQGRYGKGVVAWDMPGKSTLA
                     AVASGKPNHVATVHMSKGAPKSTRLDAAGLRKRAAGKGDVVIEVKPGESITSIAVGWA
                     LESYVKVAKAEEPKSKKEGKPKAAKAPAKKTAKPVAKAKGKPAKKAPAKAKPKKKK"
BASE COUNT          787 a          837 c          774 g          911 t
ORIGIN      
        1 gttctatggc gcgcagattc ttatcaatcg cgagaatgtt tttacagcct attacgacga
       61 agaacgggca gataacacta ccgatgcgct ctctgccttg aaacaatttg ccgataaaaa
      121 accatgctgg agcgatgaaa catccccatc tgggtatgtg gtgccgctcg ggttgctgaa
      181 tcagtatcag gtccagacca gagccggtgc ttttcttgaa ggtcaaccca atgtggtgcg
      241 cggagtctac gctgcagata tctgtgactt cggcgcaacc ttcgtcgatg cacgcaactc
      301 gcccattctc gaagcagatt accccgacgt gatggataag gttcgagtgg cgtggcgcat
      361 cccagagatc attccatatg aaaatatttc aatggctgct tctttgcctt ttgaaatgcg
      421 ccgttctgtg caacgttctt tgatcgactt tatggtcaca gccgatggca aagcggttat
      481 gcaaactatt tatggcttcg acgaaatgct gcctattgaa gatgcttcgt atgcggagtt
      541 catcaagtac gtgaatgcct cgggcttgaa tcttttggat ttgatagaat aaaacgatgt
      601 ttaaaagagt aatccgtttc ctttggcgga ctctgcttgt gctgagtgct ctcaccgcca
      661 ttggattgtt tatgcccaga tggattatgg cattattcgc tgctccacga accttcacag
      721 tggaagatgt acctgcaacc aatgttgtga ttgtgttcgg cgccggactc ttgcgcgatg
      781 gctctgccgg accggtactc agtgaccgaa tgttgactgc cgttaatttg tatcatgctg
      841 gcaaagtaga aaaaatcttg gtcagtggtg acaatcgctt tattgagtac aacgagccgg
      901 aagcgggtcg tcaatatgct ttggagcatg gtgtacctga tgaagacatt gtgctcgatt
      961 atgcaggtag gcgtacctac gatacttgtt atcgtgccaa gcatatcttc ggcgtgaatg
     1021 aagccattct cgtcacacaa gattttcaca tgccgcgcgc attggcgctt tgtaattggt
     1081 ttggtcttaa tgcagtaggc gtagaatcga ataaccgtta ctttctaaaa cgttcgcagg
     1141 tttggtggaa tttccgtgag acctttgcgg tctttcaagc cgcatgggat ttgatggtca
     1201 ccaagccgtt acctgtaatg ggtgaaccag aacctataaa ctaatatcac atgagtgaca
     1261 accaaaggag aactctatga atcgcgaaga agctttagcc cttgtacgtg aatatgtcaa
     1321 aagcgaaagt ttggtacggc atatgttatc ggtagaagcc gccatgcgtt tctatgccga
     1381 aaagtttggg gaggatgttg aatcctgggg attggttggt ttgttacacg attttgattg
     1441 ggagattcac ccaaccttgg aagagcatcc acaagcgggt gcgcccatgc tgcgcgaacg
     1501 cggtgtgcca gaagacatta ttcaagatat tttaagccat gcagatcact taaacatgcc
     1561 gcgcgatacc atgcgccgta aagccatttg tgcctgcgat gaagtgacgg gtcttatcac
     1621 cgccgttgcc ttggttcgcc cctcccgttc tttgtacgac cttgaagcca gctctgtcaa
     1681 aaagaaatgg aaagacaaag cctttgctgc cggaaccaac cgcgaagaga tggaacatgc
     1741 cgccaaggaa tttggcattg acgtgtggga acacgttggg aatgtgatcg tcgccatgcg
     1801 caagatcgcg ccagagctgg atttagttgg gaatattcca aaccccagtt aagcttttaa
     1861 aagccacaaa cgtgcctctt caatatcact gaagacgcga tagggaatcg accgattctg
     1921 ggaagccgtt tcgaaaaacc tcatatcggc ttctagtttt tgattttgtg catcatagac
     1981 caatgccacc ctagagctga ccttgtcttg tttcatgcga gtatcacgca tggcaacagc
     2041 tcgttcaaaa atatgcgcca tggataggtc aaatttcagt tctcgaatat cgagtaatat
     2101 tttacgcaat ccgctttcgg tgactgtctg cataatctgt tgaaccatca aattgtcctt
     2161 atcgctctcc caatcccccg cagcatggac caagatcaat ccattggctt gatccaattg
     2221 aatggtgtat tccatgatat tctccttgct acgattataa ctagttccac aacaaaactc
     2281 ccatgaagca cttcatggga gttttatcta ttatttcttc tttttaggtt tagcctttgc
     2341 aggagctttc tttgccggtt ttccttttgc ctttgcaaca ggtttagctg tttttttcgc
     2401 aggtgccttt gccgctttcg gctttccttc tttcttgctc tttggctctt ctgcttttgc
     2461 caccttcaca taactctcca aagcccatcc cactgcaatg gaggtaatgc tctcacccgg
     2521 cttgacttcg atcactacat cgcccttgcc agcggcgcgt ttacgcagac cagccgcatc
     2581 caaccgtgtg gatttcggcg cgcctttgct catgtgaacc gtagcaacat ggtttggctt
     2641 cccagaagcc actgccgcca aggtcgattt acccggcata tcccaagcca ctacaccctt
     2701 gccataacgt ccctgcacgg ggaagtcttt ctggtctaca cgtttggctt tgccttcgtt
     2761 ggtcaacaag aagatatcgc catcagcagg caaaatttcc ataccgacca cttcatcttt
     2821 atcttcgagc ttcataccgt tcacgcctgc tgccacaagt cccatcgggc gcacatcctc
     2881 ttccttgaag cggatcgcca tgccgctggc agtgacgagc aagatatctt tctttccgtc
     2941 cgtgatacca acagaattga gcttatcgcc ttcattgacc ttcaccaaca caaaggtttg
     3001 cgacgatggg cccggcaatt ccgtgaccag actcttcttg atcatgccga agcgtgtagc
     3061 ggttaacaca catacttcct cacccaacgc agatttgctc gcgggcaagg caaagaccgc
     3121 cgccagcgca tcattgtcac tcaatggtga aacacggtgc catggcacgc cctgactcaa
     3181 cttactcgct tcggggatca agtgcgcagc aaccgccgcg gctttcccga atttgcttac
     3241 aaaatacaca gtatcggtag tcgatgcctt gaccaggaat ctcggcgcat cattaccgga
     3301 gtgcttagg
//
LOCUS       DAOQJE010000347         3308 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_18800_length_3308_cov_6.771903, whole genome shotgun sequence.
ACCESSION   DAOQJE010000347 DAOQJE010000000
VERSION     DAOQJE010000347.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3308)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3308)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3308
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18800_length_3308_cov_6.771903"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..358)
                     /locus_tag="PLT08_09840"
     CDS             complement(<1..358)
                     /locus_tag="PLT08_09840"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559258.1"
                     /note="Catalyzes the transfer of electrons from NADH to
                     quinone; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit D"
                     /protein_id="HNE04808.1"
                     /translation="MTQLQEVSVEQFKHLVSERALTGETMLLNMGPQHPSTHGVLRLL
                     LELDGETVVNCIPDIGYLHTGIEKNMEAKTYQKAEVMTDRLDYMNPMGNNLSYVMAVE
                     KLVDLDVPPRAQALRVI"
     gene            complement(362..874)
                     /locus_tag="PLT08_09845"
     CDS             complement(362..874)
                     /locus_tag="PLT08_09845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C"
                     /protein_id="HNE04809.1"
                     /translation="MEKNLEPIVKDLQTKFGATYEEFRGEVQCMVKAENIVDVLKTLR
                     DEHSFELLSVLNAIDYYPQTSPRFHVVYQLNSLSKNLRVQVRVPVNDDQPKVPTVTGV
                     FKTGMWREREVFDMFGIEFEGHPDPRRILMPEGTEGHPLRRDFPLGYEEPQFTFNLEE
                     IDLRKPYAKE"
     gene            complement(925..1419)
                     /locus_tag="PLT08_09850"
     CDS             complement(925..1419)
                     /locus_tag="PLT08_09850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit B family
                     protein"
                     /protein_id="HNE04810.1"
                     /translation="MGVEQKLGNMGVVTTTLENVVNWSRTNAMWPMLFGLACCAIEMM
                     AAQAAHYDMSRFGMELMRASPRQSDLMIVAGRVSKKMGPVLRRLYDQMPEPKWVIAMG
                     DCASCGGVFNNYAIYQGVDEVVPVDVYVAGCPPRPEALIHGVMTLYEKVNAMKLAEKP
                     AESK"
     gene            complement(1410..1766)
                     /gene="ndhC"
                     /locus_tag="PLT08_09855"
     CDS             complement(1410..1766)
                     /gene="ndhC"
                     /locus_tag="PLT08_09855"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit A"
                     /protein_id="HNE04811.1"
                     /translation="MLLEYIAIGVMILVATLIAFIAIGLGELFGPKKKSAGKDTPYES
                     GMNPYGEGTRRMPVRFYLIAVLFILFDIEVVFFLPWAIAFRKLGLFGLIEMVVFITVL
                     LIGYVYAWKKGALEWE"
     gene            complement(1968..3308)
                     /locus_tag="PLT08_09860"
     CDS             complement(1968..3308)
                     /locus_tag="PLT08_09860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HNE04812.1"
                     /translation="MYLREIGQVKLLAADSEFRLATLIEGDRLISTLNKLKQRKGVSQ
                     AASTYHSVLSEALTSWDRLVEDTERLEHDLPNLALLLAEAQSLHAGWEFDSPSYLRAY
                     LDNGQWGSDHLWDNLARHAYSVFLSLYLLPYPYAEWLLSHVRVHQGFPAQRTLFRNLP
                     SDEELISEMESVHLRAIDAQQALVRANLRLVVSVAKRYLGRGIPLLDLIQEGNMGLLR
                     AIGKFDPRRGFKFSTYATWWIRQSINRSIAEQARTIRIPVHLFESISRILRAQRHLTQ
                     VLGREPTNEDIALEVGYLSAVDVQSILRAHAESKPVHPELQRKLDVASQKVTRALRSA
                     EETISLEGPVGDDESSALGDFIEDEDALSPMDAAAREMLREQITHALESLSDRERDVL
                     ELRFGLKDGKEHTLEEVSRFFDVTRERVRQIEAKALRKLRHPSKSRELRDYLGD"
BASE COUNT          890 a          844 c          778 g          796 t
ORIGIN      
        1 ggatcacacg caaagcctgc gcacgcggcg gcacatccag gtctaccaac ttttctacag
       61 ccatcacata ggaaaggtta tttcccatgg ggttcatata atcaaggcgg tcagtcatca
      121 cttccgcttt ttggtaggtc ttggcttcca tattcttttc aataccggta tgcaaatagc
      181 cgatatcagg aatacagttc accactgttt cgccatcgag ttccaacaac aaacgcaaca
      241 caccatgcgt ggatggatgt tgcggaccca tgttcaagag catggtctcg ccggtcagcg
      301 cgcgctctga aaccagatgt ttaaattgct ctacactcac ttcttgtagt tgtgtcataa
      361 attattcctt tgcatatggc ttgcgaaggt cgatctcttc caagttgaaa gtgaattgcg
      421 gttcttcgta gccaagcggg aagtctctgc gcagcgggtg tccctcggtg ccttcgggca
      481 tcagaatgcg gcgcgggtct ggatgacctt cgaactcgat gccgaacata tcgaagactt
      541 cacgttcgcg ccacataccg gtcttgaaca cgccggtcac ggtcgggact ttaggctggt
      601 catcgttgac agggactcgc acctgcaccc gtaaattttt tgagagtgaa ttgagctggt
      661 acacaacatg aaaacgaggt gaggtttgtg ggtagtaatc aatggcatta agcactgaga
      721 gtaattcgaa ggaatgttcg tcgcgcaagg tctttaacac gtcaacgata ttctcagctt
      781 tcaccataca ttgcacttca ccacggaact cttcgtacgt tgcgccgaat ttcgtctgca
      841 gatcttttac aataggttcg aggttttttt ccataaatac acctaagacc tgacaggtct
      901 tgaaatctgt caggtctgtt ttttttattt cgattctgcg ggtttttctg ccagcttcat
      961 cgcgttgact ttttcataca aggtcatcac gccgtggatc aaggcttcag ggcgcggggg
     1021 gcagccagcc acatacacgt ccacgggcac aacttcatcc acaccttgat agatggcata
     1081 gttattgaag acgccaccgc aagaagcgca atcacccatg gcgatcaccc acttgggttc
     1141 gggcatctgg tcgtacaaac ggcgaaggac gggtcccatc tttttggaga cacgcccagc
     1201 cacaatcatc aaatcagact gacgagggct ggcacgcatg agttccatac cgaagcggct
     1261 catatcataa tgggcggctt gagcagccat catctcaatg gcacagcaag ccaaaccgaa
     1321 caacatgggc cacatggcgt tggtgcgtga ccagttcaca acattttcaa gcgtggtggt
     1381 taccacgccc atatttccga gtttttgttc tactcccatt ccaaagctcc ttttttccaa
     1441 gcatagacgt aaccaataag aaggaccgta atgaaaacca ccatctcgat caagccgaag
     1501 agaccgagct tgcggaatgc gatcgcccaa ggtaaaaaga atacaacttc aatatcaaac
     1561 agaatgaata atacggcgat taaatagaaa cgcaccggca tacgacgcgt tccttcacca
     1621 tatggattca tgcccgattc atagggcgta tctttccccg cagatttctt ttttggacca
     1681 aaaagctcac ctagaccgat cgcaataaaa gcgatcaacg tggccactaa gatcatgacc
     1741 ccaatggcaa tatattccag cagcataaac acctctatgg atgtgtgaaa tcttgcacag
     1801 ttcgcgaaag tatatcacaa aggttttttt aagcacagcg cttgtgctac attacacaaa
     1861 aatgatgatt tttgtcatgt tgggcaaaat gtaatcgaat tcatagaaaa taccccaaga
     1921 aaacaaaaaa gtgacagtct cattatgaaa actgtcactt tggtttatta atcgcctaaa
     1981 taatctctta attctcggct cttacttggg tgccgaagtt tgcggagtgc tttggcttca
     2041 atttggcgca cccgttcacg ggtcacatca aagaagcgac tgacctcttc caaggtatgt
     2101 tcttttccat ctttaagtcc aaaacgcaat tccaagacgt cgcgctcacg gtctgaaaga
     2161 gattccaagg cgtgcgtaat ttgctcacgc agcatttcgc gggcggcagc gtccatgggc
     2221 gaaagcgcgt cttcatcttc aatgaaatcg cccaaagcgc tggattcgtc atcccccacc
     2281 ggaccttcaa gcgagatggt ttcttcggca gagcgcaaag cacgagtgac cttttgcgaa
     2341 gcgacatcta acttgcgttg caattccggg tggacaggct tactctcggc atgcgcccgc
     2401 aaaatggatt gcacatccac agcagagaga tagccaactt ccaaagcgat atcttcgttc
     2461 gtgggttcac gacccaaaac ctgcgtgagg tgacgttgtg cgcgcaagat gcgcgaaata
     2521 gactcaaaga gatgcacggg aatgcgaatg gtgcgcgctt gttcggcgat ggaacggtta
     2581 atggactgac gaatccacca agtggcatag gtgctgaatt tgaatccgcg ccgtgggtca
     2641 aacttgccaa tggcacgcag aagccccata ttgccttctt ggatcaagtc taataacgga
     2701 atgccacgcc ccagataacg tttggcaacg ctcactacca aacgcaaatt agcgcggacc
     2761 aatgcctgtt gagcatcgat ggcgcgcaaa tggacggatt ccatttcaga aatcaactct
     2821 tcatcggatg gcaaattacg gaacaaggtc cgctgagcgg gaaagccttg atgcacacgc
     2881 acatgcgaca acaaccattc cgcataggga tatggtagta gatacaggct taggaaaaca
     2941 ctataagcat gacgcgccag gttatcccat aggtggtcac tcccccactg accgttgtcg
     3001 aggtaggcac gcagatagga aggagagtca aattcccatc cggcatgaag cgattgagcc
     3061 tctgccagca agagcgccaa gttgggcaag tcatgttcaa ggcgctcggt atcctccacc
     3121 aatcgatccc aagaagtaag cgcttcagaa agtacggaat gataagtgga cgccgcctgc
     3181 gaaacccctt tacgttgttt gagtttattc aaagtggaaa taaggcggtc accttcgatc
     3241 agagtggcaa ggcgaaactc gctatctgcc gccaataatt tgacctgccc gatctcgcgc
     3301 aaatacaa
//
LOCUS       DAOQJE010000348         3283 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_19025_length_3283_cov_5.951673, whole genome shotgun sequence.
ACCESSION   DAOQJE010000348 DAOQJE010000000
VERSION     DAOQJE010000348.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3283)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3283)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3283
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19025_length_3283_cov_5.951673"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..770)
                     /locus_tag="PLT08_09865"
     CDS             complement(<1..770)
                     /locus_tag="PLT08_09865"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNE04813.1"
                     /translation="MMVDSYKPYISGITNYVEVNKQYLEKAGHEVFVFTFGDLEYEDT
                     EPNIVRSPGLPLADTGFYLSMRYSRQAKKLLQTMDVVHVHHPFLSGRLALRYCRPAGI
                     PIVFTNHSRYDLYAQAYLPAMPEEVSIGMLQAYMPPFCKAVDLVITPSAGMARVLREL
                     KVESDVEIVPNGVDLTSFQNAHPLEREPFGYKPDDILIVYAGRIALEKNLPFLIESFN
                     GIANAIPNVHLILIGGGVQQYEEEIRDVVKAQMNSDRI"
     gene            918..1112
                     /locus_tag="PLT08_09870"
     CDS             918..1112
                     /locus_tag="PLT08_09870"
                     /inference="COORDINATES: protein motif:HMM:NF014636.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoaD/ThiS family protein"
                     /protein_id="HNE04814.1"
                     /translation="MPVKLILRSKEFEVKAGMALLDALKKCNIVPESVIATRDGELIT
                     EDELLKDGEVIKLVAVISGG"
     gene            1116..2051
                     /locus_tag="PLT08_09875"
     CDS             1116..2051
                     /locus_tag="PLT08_09875"
                     /inference="COORDINATES: protein motif:HMM:NF013348.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNE04815.1"
                     /translation="MKCKKCGEKASVNMRQHRMALCKEHFLEWIPEQTERFIKKYNMF
                     TRDEKVLVAVSGGKDSLALWDILIRLGYKADGLYLGLGIDGGVQYSDESHRLTEEFAR
                     KNNLTLHVVDIEKEYGHSIPVLSEISFRGKGKPCSVCGLTKRHEMNRIARDLGYDVLA
                     TGHNLDDEAAVLFGNTLQWSPDYLLRQGPVLQSKQGLARKVKPLCRFYEREMTAYSIA
                     RGIEYIYEECPFAEGATQIFYKETLNQLENTRPGIKLTFYLRFLEARQSGELFIQREE
                     TQPHIHPCEKCGQPTSAPGLCSFCRMLEKADVVTE"
     gene            complement(2058..3047)
                     /locus_tag="PLT08_09880"
     CDS             complement(2058..3047)
                     /locus_tag="PLT08_09880"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HNE04816.1"
                     /translation="MPQSLLNGEILRERYVVREQIGQGGTGNIYLADDVRLQGRLCAI
                     KEVEHNQVLPSDVLAQAREQFFREASVLARLDHPNLPKVSDFFSEETRDYLVMDYVPG
                     KDLRQLMQEARRSNTFIPEREVLSWANQIAEALNYLHHQDPPIVHRDIKPSNIKLTPS
                     GLVKLVDFGLVKIMAPGEVTITVIQGQGTALYTPLEQYGGDDTHTDAQADIFSFGATL
                     YHLLTNEPPAEARKRFLNSRSLTPPIDINPRISSHVEKAIFWAMALHPNDRPVDVLTF
                     RDALFGKIEIPIQVDVERYGFETPRFSVFSTEQIAGYVAFGLFLVGLLATVIR"
BASE COUNT          859 a          692 c          872 g          860 t
ORIGIN      
        1 tgaatacggt ctgaattcat ctgggctttt actacatctc gaatctcttc ttcatattgt
       61 tgcacgccgc caccaataag aattaagtgg acgtttggaa ttgcattggc gataccgtta
      121 aacgactcga tcaggaatgg tagattcttt tccaatgcaa tacgcccggc gtagacaatc
      181 agaatatcat cgggtttgta gccaaatggc tcgcgctcaa gggggtgtgc attttgaaag
      241 ctggtaaggt caacaccatt aggaacgatc tccacgtcac tctctacttt gagttcacgt
      301 agcacccttg ccatgcctgc cgaaggggtg atgacaaggt caacagcttt gcaaaatggc
      361 ggcatatacg cttgcaacat tccaatgcta acttcttcag gcatggctgg gaggtaggct
      421 tgtgcataga gatcgtaccg tgagtgattt gtaaagacga tagggattcc ggcaggacgg
      481 caatatcgca aggcgagacg cccgctcaag aatggatgat gaacatgcac gacatccata
      541 gtttgcaata gcttttttgc ctgacgtgaa taccgcatgg agagataaaa gcctgtatct
      601 gccaatggta agccagggct gcggacgata ttgggttcgg tatcttcgta ctcaaggtcg
      661 ccaaaagtaa agacgaacac ttcatgcccg gctttttcga ggtattgctt gttgacctca
      721 acgtaattcg tgatgccgct gatatacggt ttgtagctgt cgaccatcat tcctattcgc
      781 ataatatgat gctacggcat gcaggtggga atgtcaaggt gggttttgtg catggtagaa
      841 taaccgtcct tcaagggtcg cagagatagt gatgaaggtg tattatcaat ataagcaaag
      901 caacaggagc gaaccccatg cccgtcaagt tgattttaag aagtaaagag tttgaagtta
      961 aagccggaat ggctttgtta gatgcgttga aaaaatgcaa tattgtgcct gaatctgtga
     1021 ttgccactcg tgatggtgag ttgattacag aagatgaact tctaaaagat ggggaagtta
     1081 ttaagttggt tgcagtgatt tcaggtggtt aagcgatgaa gtgtaaaaag tgcggtgaga
     1141 aagcttcagt caatatgcgc cagcatcgca tggcgttgtg taaggagcat tttctagaat
     1201 ggattcctga acaaactgag cgttttatca aaaaatacaa tatgtttacg cgagacgaaa
     1261 aggttcttgt cgccgtctca ggcggcaagg actcgttggc gctttgggat attcttatcc
     1321 gccttggcta caaggcagat ggtttgtatc ttgggttagg cattgatggt ggtgttcaat
     1381 actccgatga gtctcatcgc ctgacggaag agttcgcgcg caagaacaac ctcacattgc
     1441 atgttgtgga tattgaaaaa gaatacgggc attccatccc tgtgctttcg gagatcagtt
     1501 ttcgcggcaa aggcaaacct tgttcggtat gcgggctaac caaacgtcat gaaatgaacc
     1561 gcatcgcacg agacctgggc tatgatgtgc ttgcaaccgg tcataacctg gatgatgaag
     1621 cggcggtctt atttgggaat accttgcagt ggtctcctga ttatcttctt cgtcagggac
     1681 ctgtgttgca gtcaaagcag gggttggcgc gtaaggtcaa gccgttgtgt cgtttttacg
     1741 aacgcgaaat gacagcatat tccatcgcgc gtggcattga atacatttat gaggaatgtc
     1801 cattcgcgga aggggcaact cagatctttt ataaagagac attgaatcag ctggaaaata
     1861 ctcgcccggg catcaagcta acattttatt tacgttttct ggaagcaagg cagagcggag
     1921 agttgttcat tcaacgagaa gagacccagc cgcatattca cccatgtgaa aaatgcggtc
     1981 agccgacctc tgcgccgggg ctttgttcgt tctgtagaat gttggaaaag gcagacgttg
     2041 taacagaata gaccttgcta ccgaatgacg gtggcaagta accccacaag aaatagaccg
     2101 aaggctacat accccgcgat ctgctctgta gaaaaaacgg agaagcgcgg ggtttcgaat
     2161 ccgtagcgct ctacatctac ttgaattgga atctcgatct ttccaaacaa cgcatcgcgg
     2221 aaggtgagca catcaacagg gcggtcattg gggtgtaatg ccattgccca gaagatggct
     2281 ttttccacat gcgagctgat gcgtggatta atgtcaatgg gtggagtcag gctgcggcta
     2341 tttaagaagc gtttgcgcgc ctcggcaggc ggttcattcg tcaaaagatg atagagagtc
     2401 gcgccaaagg agaaaatatc cgcttgtgca tcggtgtgcg tatcatcccc tccatattgt
     2461 tcgagcggcg tgtagagtgc agtgccttgc ccttgaatga cggtgatggt cacttcgcca
     2521 ggcgccatga ttttaaccaa accaaaatcg acgagtttaa ccaagccgct aggagtcagt
     2581 ttgatattgc tgggcttgat atcacgatgc acgatcggcg ggtcttgatg atgtaagtaa
     2641 ttgagcgcct cagcgatctg atttgcccaa ctgagcactt ctcgttctgg gatgaaggta
     2701 ttgcttcggc gtgcttcctg cattagttga cgcaggtctt tgccggggac gtaatccatc
     2761 acaagatagt cacgagtttc ttcggagaag aagtctgaaa ctttggggag gttggggtgg
     2821 tcgaggcgtg cgagaacaga agcctcgcga aagaattgtt cgcgggcttg tgcaagcacg
     2881 tcggatggaa gaacctgatt gtgttctact tctttgatgg cacacagacg cccttgtaga
     2941 cgaacatcgt ctgcaaggta aatattgccc gtgccgcctt gcccgatctg ttcacggaca
     3001 acgtagcgtt cgcgcaggat ctccccgttc agtaatgatt ggggcaaaac ttctccttct
     3061 tggttgaaag cggctgcaag agttgagtga tattttcggc gtgtatcact tttgcaatcc
     3121 cgaaaagggg atttgcaata tataatcgtg cacagaaacc acttttctgc tatattataa
     3181 acgcctttgc aatggatagc acaattaaat ctataacaaa ggcgatgagg agattatgga
     3241 acgcgaagag gaatatccca ttcttgttgc acaagatggt ccc
//
LOCUS       DAOQJE010000349         3283 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_19026_length_3283_cov_5.428748, whole genome shotgun sequence.
ACCESSION   DAOQJE010000349 DAOQJE010000000
VERSION     DAOQJE010000349.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3283)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3283)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3283
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19026_length_3283_cov_5.428748"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(46..822)
                     /locus_tag="PLT08_09885"
     CDS             complement(46..822)
                     /locus_tag="PLT08_09885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE04817.1"
                     /translation="MTHRTFSPRNLLFFLASFLAFVSIACATTAPSTSTSPTAVVDVP
                     ATATAEILIPVTGGGDEAAPALPEFRRLTLEYPPVMKVGSGSDVIILTLEVDELGNIT
                     PTAQIDGNVTQGEVVEIPNLYATHNVTAEAYYEVSGLTVTPKGSTFQPLKQGERIRFT
                     WSVQPQEVGTYGGTIFLFLNFENRETGEKDRKEISIQIFDIKVVDFFGFSTNFVKTSG
                     VVGSVLGVIVGFPFFDDIVKYLLGRLTSEKGKPGRSKRKK"
     gene            complement(819..992)
                     /locus_tag="PLT08_09890"
     CDS             complement(819..992)
                     /locus_tag="PLT08_09890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE04818.1"
                     /translation="MKQIRLVLAIILLTISIALLVWGFSPNPHETKIQMIAPTEMQLP
                     TPSSLQLNPEPVA"
     gene            complement(989..1738)
                     /locus_tag="PLT08_09895"
     CDS             complement(989..1738)
                     /locus_tag="PLT08_09895"
                     /inference="COORDINATES: protein motif:HMM:NF026257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HNE04819.1"
                     /translation="MESLDLVQEMYRLVEAANAKKIQLRAIGGLAVQTHNKTDHPLFK
                     REFADLDFCIPLKQRREFEAFMPAMGYSPHKQFNVLNGDQRQIYYHNPTEMKVDIFVG
                     HFEMCHKIPLEERLNADPVTIPLAELFLSKAQIIELNRKDAFDLTSVLLNNVTGQGDN
                     ETINLKILAQLGSADWGLYKTTSINLDKVEQMLLKNEIPLNEEERNIVIGRINDIRRT
                     FAEMPKPVAWQLRDRVGTRVKWYIEVEEVQR"
     gene            complement(1777..2676)
                     /locus_tag="PLT08_09900"
     CDS             complement(1777..2676)
                     /locus_tag="PLT08_09900"
                     /EC_number="2.7.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013775.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="choline/ethanolamine kinase family protein"
                     /protein_id="HNE04820.1"
                     /translation="MSVIEEIAARVEEWKGQNVVIDQLSGGLTNTNYKITVNGKPYFV
                     RVPGASTELLAIDRNNEYHNTSAAATAGVAPKVLQHFPEYHVMVIEFLDGKTMSKDSL
                     NAAGMPTRMAHAIKKLHAGPRFLTDFNMFRLTEYYLSLCRERAIKIPKGYPERMSTVN
                     EIEKAMSVKPLATVPCNNDLLAENYIDDGKQLWLIDYEYSGNNDPTFELGNTCQEMQF
                     NDDQIKEVCAAYFGDATPQMVARMKLNMIMSDVGWGLWAAIQANISTIDFDFWGWALE
                     RWGRAEEKMDSKEFSVWMKDVVQ"
     gene            complement(2748..>3283)
                     /locus_tag="PLT08_09905"
     CDS             complement(2748..>3283)
                     /locus_tag="PLT08_09905"
                     /inference="COORDINATES: protein motif:HMM:NF024910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HNE04821.1"
                     /translation="AAAFDRLLPEKAAELNDRGTPVNAMLYMILPAILVAALYAYDVA
                     GFKSITLAATQGIAIMYFGTAIAAIILPYKKKELYEASPIAKMKVAGIPLITVAGLIF
                     GGFLLFLLVEWFFDPWLNSAGVPPGLYGISIANTNSMIFLGSCYAIAAAIYYGFKSKR
                     KSEGIDMDKVTAEIPVE"
BASE COUNT          809 a          795 c          817 g          862 t
ORIGIN      
        1 aattagcccc ccattaatgg ttgtcaattt tttgggtgat atttgtcact ttttcctttt
       61 tgacctgccc ggcttgcctt tttctgaagt caaacgcccg agcaggtact tgacgatatc
      121 gtcaaaaaat ggaaagccaa caatcacacc caaaaccgag ccaaccacac cagaggtctt
      181 cacaaaattc gtagaaaagc caaaaaagtc caccactttg atgtcaaaaa tttggatgga
      241 aatttctttg cggtcttttt cgccagtctc gcggttttca aaattcaaga aaaggaaaat
      301 cgtgccgccg tatgtcccca cttcttgagg ttgcacactc catgtaaaac gaatgcgctc
      361 cccttgtttc aacggttgaa aagtagagcc tttcggcgtc accgtcaacc ctgaaacttc
      421 gtaataggct tcagccgtca cattatgggt tgcataaaga tttggaattt caacgacctc
      481 cccttgcgtt acattcccat cgatctgggc agttggggtg atattcccca gttcatccac
      541 ttccaaggtt aggatgatga catcgctgcc tgagccgacc ttcatcacgg gcggatattc
      601 caaagttagg cggcgaaatt ctgggagggc tggcgccgcc tcatctcctc cgccggtcac
      661 tgggatgaga atttcggcag tagcagtcgc tggaacatcc acaacggcgg tgggagaagt
      721 ggaagtggac ggagcagttg tagcacaagc aatgcttaca aaagcaagga agctcgccaa
      781 aaagaacaaa aggttgcggg gagaaaaagt tcgatgggtc atgcaacagg ctccgggttg
      841 agctgaagcg aagaaggcgt cggcagttgc atttccgttg gggcgatcat ctgaattttt
      901 gtctcatgcg ggttgggaga aaatccccac accaggagtg caatggaaat cgtcaaaaga
      961 ataatggcaa gcacgagacg gatttgtttc atcgctgaac ctcttccact tcaatatacc
     1021 acttgacgcg tgtgccgacc ctatcgcgta gttgccaggc aacaggtttg ggcatttcag
     1081 caaaggtgcg gcggatatca ttgatgcgcc caatcacaat attgcgttct tcttcattca
     1141 acgggatttc attcttcaac aacatttgtt ccactttgtc taaattaatg gacgtggtct
     1201 tgtacaaacc ccaatcggcg ctgccaagct gggctaggat cttgaggttg atcgtttcgt
     1261 tatcgccttg cccagtgacg ttgttcaaca aaacggatgt gaggtcgaag gcatctttgc
     1321 ggttgagttc gatgatctgc gctttggaaa gaaaaagctc ggcaagcgga atggtgaccg
     1381 ggtcggcatt gaggcgttct tcgagcggaa ttttgtggca catctcaaaa tgtcccacaa
     1441 aaatatccac tttcatttcg gtggggttgt gataatagat ctggcgttgg tcgccattga
     1501 gcacattgaa ttgcttatgc ggggaatagc ccatcgcggg catgaacgcc tcaaactcgc
     1561 ggcgttgttt gagcggaatg cagaaatcga ggtcggcgaa ttcacgcttg aacagtggat
     1621 gatcggtttt attgtgtgtt tgtactgcca acccaccgat ggcacgcaat tggatttttt
     1681 tcgcattcgc ggcttccaca aggcggtaca tttcctgaac taggtcgagt gattccatca
     1741 agatatttct cttcttctca ttttacttat ttcaacttac tgtacaacat ctttcatcca
     1801 cactgagaat tctttcgaat ccattttttc ttctgccctg ccccagcgtt cgagtgccca
     1861 gccccaaaag tcaaaatcaa tagtggagat attcgcctga atggctgccc ataaacccca
     1921 gcccacatca gacataatca tgttgagttt catgcgcgcc accatttgcg gagtggcatc
     1981 gccaaaatac gccgcacaga cttctttgat ctggtcgtcg ttaaattgca tttcctgaca
     2041 agtattgccc aactcgaagg ttgggtcgtt gttgccactg tattcataat cgatcagcca
     2101 aagctgtttg ccatcgtcga tgtagttttc agcgagtagg tcgttattgc aaggaacggt
     2161 tgccaagggt ttaacggaca tggctttttc gatctcgttc acggtagaca tccgctcggg
     2221 atagcctttg gggattttga tagcccgttc acggcacagg ctgagataat actccgtaag
     2281 gcggaacatg ttgaagtccg ttaagaagcg cggaccagcg tgcagttttt taatggcgtg
     2341 tgccatacgt gtgggcatac ccgccgcatt gagcgaatcc tttgacatgg tcttgccgtc
     2401 gagaaattcg atcaccatga catgatattc agggaagtgt tgcaacactt tcggcgcgac
     2461 cccggctgtg gcggcggcac tggtgttgtg atattcgttg ttgcggtcaa tggcaagaag
     2521 ctcggtgctc gcccccggca cgcggacaaa atacggtttg ccattgacag tgatcttgta
     2581 attcgtattg gtcagtccac ccgaaagttg gtctatgacc acattctgtc ccttccattc
     2641 ttccactcgg gcggcgatct cttctatcac agacatatct gtctcctaaa cgcaaaaggt
     2701 gacagtcact tctaagccaa agtgactgtc accttctttt atagaaccta ctctactgga
     2761 atttcggcag tgaccttgtc catgtcgatg ccttcactct tgcgcttgga tttgaagccg
     2821 taatagatag cagctgcaat cgcataacaa gagccaagga agatcatgga gttcgtgttg
     2881 gcaatactga tgccatacaa accaggaggc acaccagcgc tgttaagcca tgggtcgaag
     2941 aaccattcca ccaagaggaa gagtaagaag ccaccgaaga tcaagcctgc cacggtgatg
     3001 agcgggatgc ccgctacttt catcttggcg atgggtgaag cttcgtagag ttcttttttc
     3061 ttgtagggca ggataatggc tgcaatggct gtgccgaagt acatgatggc aatgccttgt
     3121 gttgccgcaa gcgtgatgga tttgaagcca gccacatcat aggcatacaa agccgctacc
     3181 aaaatcgccg gcagaatcat gtacagcatc gcgttgaccg gtgtgccacg gtcgttcaat
     3241 tcagcagcct tttcggggag caagcggtcg aatgcggcgg cga
//
LOCUS       DAOQJE010000350         3280 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_19049_length_3280_cov_5.416124, whole genome shotgun sequence.
ACCESSION   DAOQJE010000350 DAOQJE010000000
VERSION     DAOQJE010000350.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3280)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3280)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3280
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19049_length_3280_cov_5.416124"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..305)
                     /locus_tag="PLT08_09910"
     CDS             complement(<1..305)
                     /locus_tag="PLT08_09910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE04822.1"
                     /translation="MKFSDLIRLVINNLSRRKARVALTAIGVVIGTGAVVILVSLAIG
                     LQMNANEQLYGIGDLSQISVVPAYEGGPMPMGGGSAAVAPEDVKLLTNSALQDLRAIP
                     "
     gene            complement(327..2036)
                     /locus_tag="PLT08_09915"
     CDS             complement(327..2036)
                     /locus_tag="PLT08_09915"
                     /inference="COORDINATES: protein motif:HMM:NF013955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IPT/TIG domain-containing protein"
                     /protein_id="HNE04823.1"
                     /translation="MNLARKSFLWVGVIMALLVSSIPTSVLAADLLSVTPTSVVNTAD
                     KTITVTGTGFDNTAVVLLDGSALATTFYNDQTLTAVVPAGYVPKTYNLTVTMSGGTVN
                     IPTPVSFTVTAPAPTNTPIPTPTAAPFARPQLRVSFSGTSGKDGIVSNKPFKFTVNFE
                     NAGSLPAYNTQATFTSADLVPLDTGGVAVLGSVSAGSSVSVDQRFVSNGSLSGKSIIT
                     IDVTLSYNDEKGNAYSDKFTLSASVSGGGGGSGVYYTATPTGVRSGQLIITSYGTDVD
                     PLEPGSRFNLVMTVQNMGNEKAPRVTMIVGGGSSGSSDGTPQPGGVSGGNGEFTNFAP
                     VGTSNIQSLGDLPAGGMVQAKQDLIVNVSTEPGAYPIKVTFSYLNAKGEVVNDEQVIT
                     LLVYSLPNLDISFYRPLDPFFVDQPGALPIQVVNLGKRTAVLGTITVTTDNGTVDGGT
                     SLVGSLDKGGYYTLDATLTPLQTGLAKINFVIEYTDDFNQKRTVEKTIDVNVEEGFIE
                     PTPDPNVPLDTGGMTPPTEETFLQKTWRFILGLLGLDSAPSTSSPDMQSPPTDFQSVP
                     SGG"
     gene            complement(2037..2765)
                     /locus_tag="PLT08_09920"
     CDS             complement(2037..2765)
                     /locus_tag="PLT08_09920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNE04824.1"
                     /translation="MNSSFIRVVGLKRHYEMGGTLVRALDGVDLEIPSHTFTVVMGPS
                     GSGKSSLLYLLGGLDRPTAGEIYVNGERLDQMDENALALFRRKTMGFIFQSFNLVSSM
                     TALENVAFPMQFAGVPVSQRNEKSQSLLSQVGLAERSHHKPSELSGGQQQRVAVARAL
                     VNDPVLIFADEPTGNLDTQSGVTIMQMLSDLHKSGRTVLVVTHDPRMVRFATNKIFLL
                     DGKVVTEAEYQAVALEPLQGNSGG"
     gene            complement(2767..>3280)
                     /locus_tag="PLT08_09925"
     CDS             complement(2767..>3280)
                     /locus_tag="PLT08_09925"
                     /inference="COORDINATES: protein motif:HMM:NF016764.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="YIP1 family protein"
                     /protein_id="HNE04825.1"
                     /translation="DMQNNYMQAQQSMQGPVFAYILPLVGALLGLWLGWLILGALLHF
                     GSTMFGGRGSMQSALMITAWASLPFLVRDGLRIIYMLIAQHPIQSPGLSGFTTNAVFA
                     SQLLSKVDLFYIWSIVLLIIGFGVTDNMPRNKSISNVVIVSILLLLIQSGVGSLLSGV
                     SGLAIQRPFF"
BASE COUNT          964 a          807 c          725 g          784 t
ORIGIN      
        1 gggatagcac gcaaatcttg caaagcgcta ttcgtaagaa gtttgacatc ctcaggtgca
       61 accgcggcac ttccgccccc cattggcatg ggtcctccct cataagccgg aacgacacta
      121 atctggctaa ggtcaccaat cccatataac tgttcattag cattcatctg caagccaata
      181 gctagagaca caaggatcac aaccgcacct gtgccgatga ccacaccgat cgcagtaaga
      241 gctacgcgcg ctttacgacg gctcaagtta ttgatgacca aacggattaa atctgagaat
      301 ttcatgcggt agccagattc atcatcttat ccgccactcg gcacggattg aaagtctgta
      361 ggtggagact gcatatcggg agaagaagtt gacggagcgc tgtctaaacc taaaagtcca
      421 agaatgaagc gccatgtttt ttgtagaaat gtctcttcag ttggcggagt cattccaccc
      481 gtatccagag gaacgttcgg gtcaggtgtt ggctcaataa agccttcttc aacattaaca
      541 tcgattgttt tctcaacagt acgtttttga ttaaagtcgt ctgtatattc aataacaaaa
      601 tttatttttg cgagtccggt ttgcagcgga gttaaggtcg catccaaagt gtaatatccg
      661 cccttatcca atgaaccgac caaagatgta ccgccatcca ctgtgccatt atctgtggtg
      721 acagtgatgg ttccaagcac agcggtgcgt ttccccaagt taacaacttg aatcggcaac
      781 gcgccgggtt ggtcaacaaa aaatggatca agcgggcgat agaaactaat atcaagattg
      841 ggcaagctgt aaaccaacaa agtaatcacc tgctcatcat taaccacttc gcccttggca
      901 tttagataag agaacgtgac tttgatagga tatgcacccg gttcagttga aacattgacg
      961 atcaggtctt gcttggcttg aaccatgcct ccggcaggta aatctcctaa cgattggata
     1021 ttcgatgtcc ctaccggcgc aaaattcgtg aactccccat ttccaccaga aacaccgcct
     1081 ggttgcggag tcccatcgct agaaccgcta gaacctccac caacgatcat cgtaacgcgc
     1141 ggcgcttttt cgttacccat attttgaacg gtcatcacca gattaaatcg agaccccggc
     1201 tcaagcggat ccacatctgt gccatatgaa gtaatgatta actgcccgct tcggactcct
     1261 gttggagtgg ctgtgtaata gacgcctgat ccgccgcccc cgccggatac agaagcagaa
     1321 agtgtgaact tgtcagaata ggcattgcct ttctcgtcat tgtagctcaa tgtgacatca
     1381 atcgtaataa tagactttcc agataaagac ccattggaaa caaagcgctg gtcgaccgat
     1441 acgctagaac ccgcagagac acttccaaga accgctacac cgcccgtatc caaagggaca
     1501 aggtctgcag atgtaaaggt tgcctgggta ttgtatgccg gcaaagagcc tgcattttca
     1561 aaatttacag tgaacttaaa tggtttgttg gaaacaatgc catctttacc gcttgtacca
     1621 gaaaaagata cacgtaactg agggcgtgca aatggtgcag cggttggtgt tggaattggt
     1681 gtatttgttg gtgccggtgc tgtaactgta aaagaaacgg gtgtagggat attaacagtt
     1741 ccaccagaca tcgtcaccgt aaggttatat gttttcggca catacccagc agggacaacc
     1801 gccgtcaaag tctggtcatt ataaaaagta gtcgctaaag ctgagccgtc aagcaataca
     1861 acagccgtgt tgtcaaatcc agttcctgtt actgtaattg tcttatcggc agtattcaca
     1921 acagatgttg gcgttacaga caacaaatcc gcagccaaaa cagaggttgg aatacttgaa
     1981 accaaaagag ccataatcac gccgacccac aaaaaggatt ttcttgctaa attcatttat
     2041 cctccagaat tcccctgaag cggttctagt gcaacagcct gatactcagc ctctgtaacc
     2101 acttttccat ctaacaaaaa tatcttattg gtcgcaaagc gcaccatgcg tggatcgtgc
     2161 gttaccacca gcacagtccg accagattta tgcaggtctg agagcatttg cataatagtc
     2221 acaccgcttt gagtatccag attgccagta ggttcatctg caaagatcaa aacagggtca
     2281 tttaccaaag cccgtgcaac agccacacgc tgttgctgcc cgcctgaaag ctcagagggt
     2341 ttatggtgac tccgctctgc cagcccaact tgagataaga gggattggga tttttcattt
     2401 cgctgtgaaa caggcacgcc tgcaaattgc attgggaaag ccacgttctc taatgccgtc
     2461 atgcttgaaa caaggttgaa cgattgaaaa ataaaaccca tcgtttttcg gcgaaaaagt
     2521 gcaagcgcgt tctcgtccat ttgatccaaa cgttcgccgt tgacgtaaat ttcgcccgca
     2581 gttgggcggt ctaacccacc aagcagatat aacaaactac ttttgcctga gccggaaggt
     2641 cccataacca ctgtaaaggt atgcgatgga atctctaaat ccacgccatc caaggcgcgg
     2701 acaagggttc cacccatttc atagtgtcgt tttaaaccaa cgactcgaat aaaagaagaa
     2761 ttcattttaa aagaatggac gttgaatcgc caaaccgctc acccctgaca ataaagaacc
     2821 aacgccagat tgaatgagaa gaagcaagat tgaaacaatt acaacattcg atattgattt
     2881 attgcgcggc atattatcag taacgccaaa gccgatgatc aaaagaacga tgctccaaat
     2941 ataaaacaag tcaacttttg aaagtagttg agaagcaaat actgcattgg tcgtaaaccc
     3001 agaaagccca gggctttgaa taggatgttg cgcaatgagc atatagataa tgcgcaagcc
     3061 atctcgtacg agaaatggca ggctcgccca tgctgtgatc atcaaggcgc tttgcattga
     3121 gcctcgccca ccaaacattg tcgagccaaa atggagcaag gcacctaaaa tcagccaacc
     3181 aagccacaga ccaagcaacg ccccaacgag cggaagaatg taggcaaaga ccggtccctg
     3241 catggattgt tgcgcctgca tgtaattatt ctgcatatcg
//
LOCUS       DAOQJE010000351         3276 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_19094_length_3276_cov_5.839180, whole genome shotgun sequence.
ACCESSION   DAOQJE010000351 DAOQJE010000000
VERSION     DAOQJE010000351.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3276)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3276)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3276
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19094_length_3276_cov_5.839180"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..287
                     /locus_tag="PLT08_09930"
     CDS             <1..287
                     /locus_tag="PLT08_09930"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="methionine--tRNA ligase; MetRS; adds methionine to
                     tRNA(Met) with cleavage of ATP to AMP and diphosphate;
                     some MetRS enzymes form dimers depending on a C-terminal
                     domain that is also found in other proteins such as
                     Trbp111 in Aquifex aeolicus and the cold-shock protein
                     CsaA from Bacillus subtilis while others do not; four
                     subfamilies exist based on sequence motifs and zinc
                     content; Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HNE04826.1"
                     /translation="AIDSLKVMFAPFIPFTSERLHGFFAYETPLFGEQYTEDVTDAIG
                     THKVLRYKPVEGLQWKPSDLKPGAKLNQPGPLFKKLEADLIEVERGRLGK"
     gene            complement(336..1520)
                     /locus_tag="PLT08_09935"
     CDS             complement(336..1520)
                     /locus_tag="PLT08_09935"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015049.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBOAT family O-acyltransferase"
                     /protein_id="HNE04827.1"
                     /translation="MGVLLILAILFFQKWMNAYDGGGSGLLGNAVPLSIFPILGLSYV
                     SFQVISYLVDVSRNGNSTELNFVNYAAYLLFFPKLVSGPLVKYKQFKDQLSNLSFDMD
                     GVAAGLRRILSGVVKRLLVANPLGVVADSVFNLPTANVAPHFAWLGLIAYTLQIFFDF
                     SGYTDIAIGLAKIMGINLPENFNFPYIAQSVGEFWRRWHMTLSGWFREYVFFPLERNR
                     TRLNNQQVNIFIVFLLTGLWHGLTLNFIVWGALHGIMIALESLGGGRWQKQIWRPFRH
                     LITMAIVMLGWVFFRSTNLGYAMEFIGRLAGNTNGLTTLPFSLTRPLPFIEPSFVITL
                     VFAVIFSLPIGQIWQKIRSGFESRVLIFQFVEDIMLLSLFLLAIGSYLSGSFKPNIYA
                     AF"
     gene            1567..1812
                     /locus_tag="PLT08_09940"
     CDS             1567..1812
                     /locus_tag="PLT08_09940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE04828.1"
                     /translation="MARPNAKPIKVMINKDIREEGCPRKIKTRLATKIKANRWMDAEK
                     YHKNKKMGKKKKRKVNVEKSIKHLRSFNKNHYKQPIN"
     gene            complement(1809..2762)
                     /locus_tag="PLT08_09945"
     CDS             complement(1809..2762)
                     /locus_tag="PLT08_09945"
                     /inference="COORDINATES: protein motif:HMM:NF028132.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE04829.1"
                     /translation="MVQGLKGGYQSFDDNFFQRQVFIQDFNRLKLALGDRIFPTVLVN
                     RDGWMVFLEDTNINDFQNSTGFTKKDADEVVEVISQCYRRAQEDGFTFLIVVAPNKET
                     IYPNIVPDQIGKTSKVSRFDVLNTELLNRSLPGMLDLRGPLIRESAKRQIYYKTDTHW
                     NGYGAYIAYEEIIKALAQDYPVLVPYPEKFFRIKERSLRVRDMAESIGTKYIMEPLIG
                     PSPEFINFTTFGVESGQKGVVAFTPDKDLPSLLMLHDSFGQTLIPFLSNNFEQAIYSN
                     VSTASVIYIDKDKLSYINPDIVILEIVERNLPFIAKYLEGC"
     gene            2991..>3276
                     /locus_tag="PLT08_09950"
     CDS             2991..>3276
                     /locus_tag="PLT08_09950"
                     /inference="COORDINATES: protein motif:HMM:NF019334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HNE04830.1"
                     /translation="MVEWIGQTVGKVRIDRYLAHGGMAEVYLGTHLTLDRSVAVKLMH
                     SYIEADPELLARFQREARAIAGLRHPNIVQIYDFDTHDKHPYIVMEYLKGP"
BASE COUNT         1033 a          757 c          692 g          794 t
ORIGIN      
        1 aagccatcga ctcattgaag gtcatgttcg cgccgttcat ccccttcact tccgagcgcc
       61 tgcacggatt cttcgcttat gagactccgc tcttcggcga gcaatacacc gaagatgtga
      121 ccgacgccat cggcacgcat aaggtgctgc gatacaaacc ggttgaaggc ttgcaatgga
      181 aacccagcga cttgaaaccc ggcgcgaagt taaatcagcc tggtccgctg ttcaagaagt
      241 tggaagcaga tttgatcgaa gtggaacgtg gaaggttagg aaagtaaact caccggaagg
      301 gcaggataat tcctgccctt ctgttttgtt cggtattaga atgccgcata aatattcggt
      361 ttgaaagaac cgctcagata ggaaccaatg gcgagcagaa aaagagacaa aagcattatg
      421 tcttctacga attgaaaaat caaaacacga ctctcaaagc cactgcgaat tttctgccat
      481 atctgaccta tcgggagcga aaaaatcacc gcaaagacca gggtgatgac aaaagacggc
      541 tctatgaatg gcagcggccg ggtcaggcta aaaggcaggg tggtcaagcc atttgtgttc
      601 cccgctaaac gcccgataaa ttccatggcg tagccaaggt tcgtcgaacg aaagaagacc
      661 catcctaaca taacaattgc catggtgatc aagtgtctga acggccgcca aatttgcttc
      721 tgccatcgtc cgccccccaa actctccaat gcgatcataa ttccatgaag cgcaccccat
      781 acgatgaaat taagcgtcag gccatgccat aatccggtca aaaggaacac gatgaatata
      841 ttgacttgtt gattatttaa tcgggtccgg ttgcgttcca aggggaaaaa tacatattca
      901 cgaaaccagc cagacaacgt catatgccat ctgcgccaga actcccccac gctttgggca
      961 atgtagggga aattaaagtt ttccggcaaa ttaattccca tgatctttgc caggccaatg
     1021 gcaatatctg tataccctga gaaatcaaaa aatatctgca aagtatatgc gatcaaccca
     1081 agccatgcaa aatgaggggc cacattggcg gttggcaggt tgaaaactga atctgcgacc
     1141 acccccaatg ggttggcgac aagcaatctc ttcaccacac cggacaatat acgtcgcaaa
     1201 ccagccgcaa ctccgtccat gtcgaaagat agattggaca gctggtcttt aaattgtttg
     1261 tatttgacca aagggccaga gacaagtttg ggaaaaaaca ataaataggc agcataattt
     1321 acgaagttca attctgtgga gttaccgtta cgagaaacat caaccagata agagatcacc
     1381 tgaaaggata cgtaagacag cccaaggatt ggaaagatgg aaaggggcac tgcatttccc
     1441 aacaaaccgc ttcctccgcc atcatatgcg ttcatccatt tttggaaaaa caatatagcc
     1501 aggatcaaaa gaacgcccaa ccacagaaac gaggatgtat tctttccgcc tgttcgtcga
     1561 atatcaatgg cacgccccaa cgcaaaacct attaaggtga tgatcaacaa agatataagg
     1621 gaagaaggct gcccaagaaa aataaaaacg agacttgcaa caaaaatcaa agccaaccga
     1681 tggatggacg ctgaaaaata ccataagaat aaaaagatgg gcaaaaaaaa gaaaagaaaa
     1741 gttaatgtcg aaaaatccat aaaacatctc cgcagcttca ataaaaatca ctacaagcag
     1801 cctattaatt aacacccctc aaggtatttg gctataaagg gcaaatttct ttccacaatt
     1861 tccaggatca caatatcggg gtttatgtac gaaagtttat ctttatcgat ataaatgacc
     1921 gacgctgtgg agacgttact ataaattgcc tgttcaaagt tattggaaag aaaagggatc
     1981 aaggtttgac caaaagaatc atgcagcatt aataaagaag gtagatcttt gtcgggtgta
     2041 aaagcaacaa cccctttttg gccggattcg acgccaaagg ttgtaaagtt aatgaattca
     2101 gggctgggcc caattaaagg ctccataata tacttagtgc cgatactttc agccatatct
     2161 cttactctta atgagcgttc tttaatgcga aagaattttt caggatacgg aacaagaacg
     2221 gggtaatctt gcgccaatgc cttgattatt tcctcatatg caatataagc gccataccca
     2281 ttccaatgcg tatctgtctt gtaataaatt tgtcttttcg cgctttctcg aattaagggc
     2341 ccgcgcaagt ccaacatgcc cggtaatgaa cgattgagca actctgtatt caatacatca
     2401 aagcgtgaca ccttagaggt cttgccgatc tggtcaggca cgatattcgg gtagatcgtc
     2461 tctttattcg gagcaacgac aataagaaac gtaaatccat cttcctgagc gcggcgatag
     2521 cattgggaaa ttacctcaac aacctcatca gcatcttttt ttgtgaaacc cgttgagttt
     2581 tgaaaatcat tgatattcgt atcttccaaa aagaccatcc acccatcccg attcaccaaa
     2641 acggtcggaa aaatacgatc tcccaaagcc aacttaagcc gattaaagtc ttgaataaac
     2701 acctgtcgtt gaaaaaaatt gtcgtcgaaa gattgatatc cacctttgag gccttgcacc
     2761 attgccacga gtaataagac aaagatcgcc aaggcaaaga gggaatgata ggtcttgtat
     2821 tttttttcga ggtcgattgg gggtttcata gattctctat tttttatgat aagcgatgca
     2881 ttatacactg taaaaatcgc cttgtatttt tagacccaat tcatcttccg tatctaattt
     2941 caataccatt tcagactact tttaccgtat ttcagttgta taatcaacgc atggttgaat
     3001 ggatcgggca gaccgtcggt aaagtccgta tcgacagata cctcgcacat gggggcatgg
     3061 cagaggttta tttagggacg catctcaccc tcgaccgctc tgtagctgtg aaacttatgc
     3121 acagttacat cgaagccgac cccgagctgc tggcccgctt ccaacgcgaa gccagggcta
     3181 ttgccgggct gcgccacccc aatatcgtcc agatctacga tttcgataca cacgataaac
     3241 atccttatat cgtaatggaa tacttgaaag ggccgt
//
LOCUS       DAOQJE010000352         3266 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_19185_length_3266_cov_6.118032, whole genome shotgun sequence.
ACCESSION   DAOQJE010000352 DAOQJE010000000
VERSION     DAOQJE010000352.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3266)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3266)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3266
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19185_length_3266_cov_6.118032"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1190
                     /locus_tag="PLT08_09955"
     CDS             <1..1190
                     /locus_tag="PLT08_09955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HNE04831.1"
                     /translation="GRIFEVTSNAYAYTGDGAAMLLRKGLPLEDMEFFQFHPTGIYKL
                     GILITEGARGEGAVLINGKGERFMPKYAPTVKDLASRDVVSRAIYTEIKEGRGIDGKN
                     YVYLDIRPETVNKYAAEDGRTNPDGSPYKITGDTVLKKIPDIVDFCRVYLGVDPVTQM
                     MPIQPTAHYAMGGIPTNKYGEVVVDEKGTTIPGLFAAGECACVSVHGANRLGTNSLLD
                     LVVFGKHAGLRAAEYAKSADWAKLPENADSAIRSELEALKSASGKENAYDIGNEMKNV
                     MFADVGIYRNEKDMNTALEKVRELQKRFKDVKVSDTGKIFNTELLNAWELGNMLEIAE
                     VVAASALNRKESRGGHSREDFPDRDDKNWLKHTLIAKKNGKLEINYKPVVITKHQPKA
                     RVY"
     gene            1199..1906
                     /locus_tag="PLT08_09960"
     CDS             1199..1906
                     /locus_tag="PLT08_09960"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008177 - succinate dehydrogenase
                     (ubiquinone) activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase iron-sulfur subunit"
                     /protein_id="HNE04832.1"
                     /translation="MEVTVKIFRYNPEKDQRGNYVTYKVEADPNDRVLDVLEYIKGKT
                     DGALSFRRSCAHGVCGSDAMRINGRNRLACKTLIRDVGDTFTVEPILGLPIKKDLIVD
                     MEPFFNNYKKVLPYFINEEPLPEGGRERLQSPEQRARFDETTKCILCAACTTSCPSYW
                     ANGEYYGPAAIVSAHRFIFDSRDQAADQRLNILAETDGIARCHTAYNCTEACPREIQV
                     TKAIGEVKMAMMTGNLD"
     gene            complement(1980..>3266)
                     /locus_tag="PLT08_09965"
     CDS             complement(1980..>3266)
                     /locus_tag="PLT08_09965"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNE04833.1"
                     /translation="NRHVSVRAFSPKRGQFATIFEDITERKQAEEKLNESYARLDFAN
                     HELKRAMHAKDEFLANMSHELRTPLNAIIGLSESMLEISANDFTPRQQKYLHTIQESG
                     QHLLEMINDVLDLAKVGAGQLTLESRTVDVDSVCQASLRMIKQLAANKNLGVHFEIDQ
                     DIDSIHADERRLKQMIVNLLTNAVKFTPATGEIGLEVRGDKLNQQVWFTVWDTGIGIL
                     KEDQARLFRPFVQVNSSRVRDVGGTGLGLALVAEMARLHGGTVSLESEPGKGSRFSFT
                     LPWSDLPVAVGSSKKTGQTGSLTFPSKNTPEKKQTILLVEDTETAVMPARDYLEAKGY
                     HVVVAQNGQEGINIANQIKPDLILMDVQMPVMDGLEAMRRLRAQAEFRDTPIIALTAY
                     AMDEDRERCLAAGATDYVSKPYKLRLLVQKIEGLLK"
BASE COUNT          798 a          765 c          829 g          874 t
ORIGIN      
        1 ttggtcgtat ctttgaagtg acttcgaatg cttacgccta taccggcgat ggtgcagcga
       61 tgttactgcg caagggactt ccgctcgagg atatggaatt cttccaattc cacccgaccg
      121 gtatttacaa gctcggtatt ctcatcaccg aaggggcacg tggtgaaggt gcggtgctca
      181 tcaacggcaa aggcgaacgc ttcatgccga agtacgcgcc caccgtgaaa gacctcgcct
      241 ctcgcgatgt ggtttcccgc gcgatctata ctgagatcaa agaaggtcgc ggtattgatg
      301 gtaagaatta tgtgtatttg gatattcgcc cagagacggt gaataagtat gccgcggaag
      361 atggacgtac caacccagac ggttctccct ataagatcac tggggatact gttctcaaga
      421 aaattcctga cattgtggat ttctgccgcg tctatctcgg cgtggaccct gtcacccaga
      481 tgatgcccat tcagccaacg gctcactacg cgatgggcgg tattccgacc aacaagtatg
      541 gtgaagtggt ggtggatgaa aaaggcacaa ccattcccgg tttgtttgcg gcaggtgaat
      601 gtgcttgtgt gtctgtgcat ggtgccaacc gcttggggac gaactctctg cttgacttgg
      661 ttgtgttcgg caagcatgcg ggcttacgtg ccgcggaata tgccaagtct gcggattggg
      721 cgaaactgcc cgagaacgct gactcagcca tccggtccga attggaggcg ttgaagtccg
      781 cttcgggtaa ggaaaatgcg tatgacatcg gcaatgaaat gaagaacgtc atgtttgcgg
      841 atgttggtat ttatcgcaac gagaaagata tgaacaccgc gcttgaaaag gtgcgtgagc
      901 ttcagaagcg cttcaaggat gtgaaagtta gcgacacggg caagatcttt aataccgagc
      961 tgttgaacgc ttgggaactt ggcaacatgc ttgagatcgc ggaagtggtt gccgccagcg
     1021 ctttgaaccg taaggaatcc cgtggtggtc attcgcgtga agatttccct gaccgcgatg
     1081 ataagaattg gttgaaacac accttgatcg cgaagaagaa tggcaaattg gaaattaact
     1141 acaagccggt tgtcattacc aaacatcagc ccaaagcaag agtctattag aggtcagcat
     1201 ggaagttact gttaagattt tccgttacaa ccccgagaaa gaccagcgcg gcaattatgt
     1261 gacctataag gtcgaagccg accccaatga ccgcgtgttg gatgtgcttg aatatatcaa
     1321 gggcaagacc gatggcgctt tatcgttccg ccgttcgtgt gcccatggtg tctgcggttc
     1381 agatgcgatg cgcattaatg ggcgcaaccg tttggcgtgc aagactctca tccgcgatgt
     1441 aggtgacacg ttcactgtcg agccgatcct tggtttgccg atcaagaaag atttgatcgt
     1501 ggatatggag ccgttcttca ataactataa gaaggtgctt ccatatttca tcaacgaaga
     1561 gcctttgccc gaaggtggac gcgaacgttt gcagtcgccg gagcagcgcg cccgctttga
     1621 tgaaaccacc aagtgtattc tttgtgcggc ttgtaccacc tcctgccctt cgtattgggc
     1681 gaacggtgag tattatggtc cggctgccat cgttagcgcg catcgcttca tttttgatag
     1741 ccgcgaccag gctgctgatc agcgcctcaa catcctcgct gaaacagacg gtatcgcccg
     1801 ctgtcatacc gcgtataact gcactgaagc ctgtccgcgt gagattcagg tgaccaaagc
     1861 catcggtgaa gtaaagatgg caatgatgac cggtaatttg gattaattgt cgctgaataa
     1921 aaggaccgcg catatgcgcg gtccttttta ttttatgtga aagttgctat taagtccttt
     1981 tatttcagca aaccttcaat tttttgtact agtaacctta acttatatgg cttactaaca
     2041 taatctgtgg caccagctgc caaacaacgc tcacgatctt catccatggc ataggcggtt
     2101 agggcaatga ttggggtatc tcgaaattcg gcttgagcac ggagtcggcg catggcttca
     2161 agcccatcca ttacgggcat ttgaacatcc atcagaatca agtcaggctt gatttggttg
     2221 gcaatgttaa ttccttcttg cccgttttgt gctacgacta catggtagcc tttcgcttct
     2281 aggtaatcgc gagcgggcat tacagcagtt tctgtatctt cgacaagcag gatcgtttgc
     2341 tttttttcag gggtattttt tgaaggaaaa gtcaaactgc ctgtttgccc tgtctttttg
     2401 gaagagccaa cagctacagg cagatctgac caaggtagtg tgaaagaaaa acgactacct
     2461 ttaccgggtt cactttcaag gctgacagta ccaccatgta aacgagccat ttctgctaca
     2521 agtgccagcc ccaaacctgt tccaccaaca tcccgtactc tcgaagagtt gacttgtacg
     2581 aacggccgga acaaacgagc ctgatcttcc ttcaatatcc ctatccccgt atcccatact
     2641 gtgaaccaaa cctgttgatt cagtttatcg ccacgcactt ctagaccgat ctcccctgta
     2701 gcgggcgtga attttacggc attggttagc aggttgacga tcatttgttt cagacgacgt
     2761 tcatcagcat gaatggagtc gatatcttga tcgatttcaa aatgcacacc aaggttcttg
     2821 ttcgcagcaa gttgtttgat catacgcaag ctggcttggc atactgaatc tacatctaca
     2881 gtgcgcgatt caagtgtaag ctgccctgcg ccgacttttg caagatcaag gacatcattg
     2941 atcatttcca ggaggtgttg tccactttct tgaatagtgt gcagatattt ttgttgcctg
     3001 ggtgtaaagt cattggcaga aatctctaac atgctttctg aaagaccaat aatggcgttc
     3061 aatggggtgc gtaattcatg actcatattt gccaaaaact catccttggc atgcatggca
     3121 cgtttcaatt catggttggc aaaatctaag cgggcgtagc tctcgtttag tttttcttct
     3181 gcttgtttgc gttcagtaat gtcttcaaag atggtggcga actgtccgcg ttttggagaa
     3241 aaggcgcgta cgctcacatg gcgatt
//
LOCUS       DAOQJE010000353         3262 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_19232_length_3262_cov_2.916121, whole genome shotgun sequence.
ACCESSION   DAOQJE010000353 DAOQJE010000000
VERSION     DAOQJE010000353.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3262)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3262)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3262
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19232_length_3262_cov_2.916121"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(225..938)
                     /locus_tag="PLT08_09970"
     CDS             complement(225..938)
                     /locus_tag="PLT08_09970"
                     /inference="COORDINATES: protein motif:HMM:NF015087.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016051 - carbohydrate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate synthase family protein"
                     /protein_id="HNE04834.1"
                     /translation="MKNRTLYVNGFEISDDSDCFVIAEIGNNHQGKLQKAMEMFKAAK
                     ECGVDAVKLQKRDNKSLLTKAAYDKPYDNENSFGATYGEHREALEFGKREYIELKKYA
                     DEIGLTMFATAFDMRSADFLAELDMPLYKIASGDLKNTPLLKHVAKIGKPMIISTGGG
                     LMEDVQRAYDAIMPINKQLAILQCTASYPAEPQDLNLRVITTYREKFPDVVVGLSDHE
                     NGIAMALAAFVLGSQNPAW"
     gene            complement(955..2352)
                     /locus_tag="PLT08_09975"
     CDS             complement(955..2352)
                     /locus_tag="PLT08_09975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE04835.1"
                     /translation="MRVLFVSANYHYYFAIDSVVRELSSRGHHVYLSTGMKEKANFPG
                     NIAMEKAQSELPNLEVHQLLRRRFLRSFAQNIRELLNFAHVLKSEDVRKWDVLIWGRF
                     FSSKWVWRLFNTPGGKAKLRNPSFLRMIRALEQHIPVHARIRTEIKNISPDVVLLLPL
                     VTPNALENEYMRAAQSLGIPTIYSMVSWDNLSTKGTFHGYPDYSFVWNKPLADELVDL
                     HGHPRETIFMTGTPRFSHLFDGSNKKEDVFSREELCRQAGMDASKPYVLYVGSTFLVN
                     SKREIERDESLIIREVAEAMLADPRTKDVNILVRPHPQNLAYLSKFLETKPRNVFVYP
                     VNGEIPDTDEKIKRYNSSIHYAAAVLGINTTAYLEVAALNRPCVTLYFNEFAETQRLP
                     HFHHLEEAGFLESARDTSEMIALLERILNGADDLAAQRHEFVKNFLKPLGSEKPSVEY
                     VADLIEEIGSKKQIY"
     gene            complement(2390..>3262)
                     /locus_tag="PLT08_09980"
     CDS             complement(2390..>3262)
                     /locus_tag="PLT08_09980"
                     /inference="COORDINATES: protein motif:HMM:NF025877.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Wzt carbohydrate-binding domain-containing
                     protein"
                     /protein_id="HNE04836.1"
                     /translation="SRAEVTRKFDEIVDFSGVEKFIDTPVKRYSSGMYVRLAFAVAAH
                     LEPEILVVDEVLAVGDAEFQKKCLGKMSDVAGSGRTVLFVSHNMPAILRLCSSAILLD
                     KGSLIMQGSPLEVTNYYLNAEQFTEGIVKWSGNNAPGDEEVRLIEVRLVDAAGKTNPV
                     LDVTKRYYLRIRYQVNGESLSFRCSVKFNTQGVTAFVTMHASDQLHDYAGIYETYVEI
                     PPNLLTEGEYTLSMSLFSYTGSRKQYTILKNFFSFQVVDYMNVESSRGDYTQSMPGVV
                     RPHLNWGIDYLGKG"
BASE COUNT          895 a          801 c          707 g          859 t
ORIGIN      
        1 gccgtcggcg ctgacgccca tgccgaaggt gccgtagccg ggcccgaggg aggggcgttg
       61 gttgaagctc tgggcgctgg cgtggggggc cgcgaacccg tgggcaagaa ggccgagcgt
      121 ggcgagggag agtgcgcgaa cgcggaagaa accagacgca gcgcgcggcg tgggcatatt
      181 gggggggctc cagtgaagat aagacgcgat aacaccgcaa gaggttacca cgcggggttc
      241 tgtgagccaa gcacaaatgc cgccaacgcc atggcaattc cattttcatg gtcagacaaa
      301 ccgaccacaa catccgggaa cttttcacga taggtggtaa taacgcgcaa attcaggtct
      361 tgtggctcag ccggatagga tgctgtacat tgtagaatcg ccaactgctt attgattggc
      421 ataatcgcat catatgcacg ctgaacatct tccatcaaac cgccgccggt actaatgatc
      481 atcggtttac cgatcttggc gacatgcttc aagagcggcg tattcttcaa atcgcctgaa
      541 gcgatcttgt aaagaggcat atcaagttca gcgagaaagt ccgcacttct catgtcaaat
      601 gcggtagcaa acatggttaa accgatctca tcggcatact tttttagttc aatatattct
      661 cttttaccga attccaatgc ctcacggtgt tcaccatatg tggcgccgaa gctgttttca
      721 ttatcatatg gtttgtcata tgcggctttg gtcaacagag acttgttatc gcgcttctgt
      781 aatttaactg catccacgcc gcattccttg gcggctttaa acatctccat tgccttttgt
      841 agtttccctt gatggttgtt accaatttcg gcgatcacaa agcaatcgct gtcatctgaa
      901 atttcaaagc cgttcacata taaggttcta tttttcatgg gtctccaatt tagctcaata
      961 tatctgtttc tttgagccga tttcctctat taagtcagca acatattcaa cagatggctt
     1021 ctcagatccc aaaggtttga gaaagttctt cacaaattca tgccgttgag cagcaagatc
     1081 atctgcacca ttcagaatgc gctcaagaag tgcgatcatc tctgacgtat ctctggcaga
     1141 ctccaaaaag cccgcctctt ccaggtggtg aaaatgcgga agccgttgtg tctcagcaaa
     1201 ctcattgaaa tacagggtaa cacaaggacg gttaagcgcc gccacttcca gatatgctgt
     1261 ggtatttatg cccaatacag cggcagcata atggatcgaa ctattgtatc tttttatctt
     1321 ttcatctgta tcaggaatct caccatttac agggtaaaca aatacatttc gcggcttggt
     1381 ctccaaaaat ttggagagat acgccaggtt ttgcggatgc gggcggacaa gaatattaac
     1441 atcctttgtc cgcggatcag caagcattgc ctctgccact tcgcggataa tgagactttc
     1501 atcccgttcg atctcacgtt tggagttcac caaaaaagtc gaaccaacat aaagaacata
     1561 aggcttgctc gcatccatac cagcctgtct gcatagctct tcccgagaaa agacgtcttc
     1621 ttttttgttc gacccatcga acagatgcga gaagcgaggc gtgccagtca tgaagatcgt
     1681 ttcacgcgga tgtccgtgta aatcgaccaa ttcatctgcc aaaggtttgt tccaaacaaa
     1741 agagtaatcg gggtacccat gaaatgtacc cttggtagac agattatccc aactaaccat
     1801 cgaatagatc gttgggatcc caagcgactg tgccgcccgc atgtattcgt tttcgagggc
     1861 atttggggtc acaagcggca gaagtaaaac aacatcgggg ctgatgttct tgatctccgt
     1921 tcggattctg gcatggacgg ggatatgttg ttccaatgca cgtatcatgc gcaggaagct
     1981 gggatttcgt agtttcgcct tcccgcccgg cgtattaaaa agacgccata cccatttgga
     2041 cgagaaaaaa cgtccccaga tcaacacatc ccattttcga acatcttcac ttttcaagac
     2101 atgtgcaaag ttaagtaatt cacgaatgtt ctgtgcaaaa ctacgcagaa aacgtcgcct
     2161 taataattgg tgaacctcca agtttggcaa ttcggactga gctttttcca tggcaatgtt
     2221 acctgggaag tttgcttttt ctttcatccc tgtggaaaga tatacatggt gtccacgact
     2281 tgaaagttca cgaaccacag aatcaattgc aaaatagtaa tggtaatttg ccgatacaaa
     2341 taaaacacgc attgagtcct ccaattcatc cgctcacaac cgatgagtat tatcccttcc
     2401 ctaaataatc aataccccaa ttcagatgcg gacggaccac gcctggcatg ctctgggtat
     2461 aatcaccgcg agaggactcc acattcatat aatcaactac ttgaaacgaa aagaagttct
     2521 taagaatagt gtattgcttt cttgaacctg tatacgaaaa aagtgacatg gataatgtgt
     2581 actcgccctc ggtcaataaa ttaggtggaa tttcaacata tgtttcataa attccggcat
     2641 agtcgtgaag ttgatcgctc gcatgcatag ttacaaacgc agtaacaccc tgggtattga
     2701 acttgaccga acagcgaaaa ctcaacgact ctccgtttac ctggtagcga atccgcaaat
     2761 aatatcgttt tgtcacatct aaaaccggat tggtttttcc agccgcatcc actagtcgca
     2821 cctcaatgag tcgcacctct tcatccccag gtgcattgtt tccagaccac tttacgatac
     2881 cttcagtaaa ttgttcggca ttcaagtaat aattggttac ttccaatggg ctgccttgca
     2941 ttatgaggct acccttatct aatagaattg ccgaagaaca taagcgcaaa atagcgggca
     3001 tgttatgact gacaaacaaa accgttctgc cagaaccggc tacatcactc atttttccaa
     3061 gacatttttt ctggaactcc gcatcgccta cagccaacac ttcatctacc actaaaattt
     3121 cgggttcaag atgagccgca acagcaaagg caagacgcac atacatccca cttgaatagc
     3181 gcttgactgg tgtatcgata aatttctcta caccagaaaa atccacaatc tcgtcaaatt
     3241 ttctggttac ttccgcgcga ct
//
LOCUS       DAOQJE010000354         3258 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_19260_length_3258_cov_6.974399, whole genome shotgun sequence.
ACCESSION   DAOQJE010000354 DAOQJE010000000
VERSION     DAOQJE010000354.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3258)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3258)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3258
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19260_length_3258_cov_6.974399"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(187..810)
                     /locus_tag="PLT08_09985"
     CDS             complement(187..810)
                     /locus_tag="PLT08_09985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE04837.1"
                     /translation="MKKTLMVVICVIFGVLYFSIPPSVAVQSRPDYHIPDTPEAKEVI
                     KAVERAYDVQAEAKFNFDIKKFPDIFINDPRFPVSSATLKTIRELTNNPSVESAGYLD
                     YKVAYYSWLIESTLHAEKVKEKAKQENRPLTAEEKKSLVDQNGRIAPVRSESPKRTLS
                     LTFLSVSVDEDVATVVLNDGPWTLEMYLVLVDKKWYVAGTKGLVFHP"
     gene            complement(836..1633)
                     /locus_tag="PLT08_09990"
     CDS             complement(836..1633)
                     /locus_tag="PLT08_09990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE04838.1"
                     /translation="MKNKRQYLKIFLLIAVVGIAFGFFVKAGAQVDVVTYLIEEFKLQ
                     SVPVKQVVVLEENPLKIQVVAVSFQNEGITFEDHLTLNSVDRIVALSAREKGYSIQSY
                     RTIIQDNKGNQLFGEERIIDVEQINSVLNRIQTNISDNSVNTLAIREVEMLLSDYKLE
                     NVSAQVDVTSLNGSRYLYIQLSLPSMDNAEKAATLLWVFHHTKYFRELEENGSQIAVY
                     RAKLLDEKGVTLMDYLYDYEINSGSWVQDERIPPLGGSPGADPAASP"
     gene            complement(1781..2338)
                     /locus_tag="PLT08_09995"
     CDS             complement(1781..2338)
                     /locus_tag="PLT08_09995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE04839.1"
                     /translation="MKKKILPVILTIALILTSCLKVKGAEAEPMTNVSESLALDLAFS
                     YSKDTRLGGWLVPPEKAYGQVMTYDEAMLFVFGKSVEPNTKEYEKRNKTIWLIVLEGE
                     FVEHVPASAGGDIPATEIIHKQMIIFLDGETGEIMREILPSPQMELPVTNLPVLQKTN
                     EEFPVLPTRAPALTEVPYPTLPAIQ"
     gene            complement(2343..2990)
                     /locus_tag="PLT08_10000"
     CDS             complement(2343..2990)
                     /locus_tag="PLT08_10000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE04840.1"
                     /translation="MKKTILAILTLALVLTSCAKAKDTEVAPVFTGGSSTVEWASKAA
                     TVEELVSEADLIVRVRVIEAPVTRVLRHELPVWGENNKIVGSETFEALFSDTVFEVVK
                     TYLGDPPSQITVMQIGGYDPTVSKGIEEVGDDPLYKVREGYILFLVNISGDQIHAPDR
                     ELYRIVNPFGRYGIDGEAVFSYGQNSGTDAVFVTSTIKELELQIEQAVDLLKRPD"
BASE COUNT          953 a          774 c          578 g          953 t
ORIGIN      
        1 cgctctcgat ctgttcgagc agggttaatt cgcggagttc ttcgtttgtt gattccatct
       61 caaatttcat tccaaaattc agtcgctgaa ttataaccca tttgagacgg ggtagagaag
      121 attctggaaa acaagaagcc acgtgacttg gacaaatcac gtggcttctt tgctataccc
      181 taaagtttat ggatgaaaaa caagcccttt ggttccagct acataccatt tcttatcaac
      241 caaaacaaga tacatctcca atgtccaggg accgtcgttt agaaccacag tagcaacatc
      301 ctcatctaca ctcacagaca gaaatgttaa agacagtgtt ctttttgggc tttcagaacg
      361 cacaggcgca atgcgcccat tctgatcaac aagtgatttt ttttcttccg ctgtcaaagg
      421 acgattttcc tgcttcgcct tttcttttac cttttcagca tgaagagtag actctattaa
      481 ccatgaataa tatgccacct tgtaatctaa gtatcctgca gattccactg acggattatt
      541 ggtcaactcc ctgatcgttt tcaaagtcgc tgaacttaca ggaaaacgcg ggtcatttat
      601 gaaaatatca ggaaatttct taatatcaaa gttgaattta gcttctgctt gaacgtcata
      661 ggctctctca acagctttaa taacttcttt cgcttctggc gtatcaggaa tatgataatc
      721 tggcctagac tgaacagcca cagatggggg aatagagaaa tacaacactc caaaaataac
      781 acaaataaca accatcaatg tttttttcat ttttgcctct aattaaggtc cataattatg
      841 gtgacgctgc cgggtctgcg ccagggcttc ctcccaatgg aggaattctt tcatcctgca
      901 cccaactacc agaattaatt tcataatcat acaaataatc cattaatgta actccctttt
      961 catcgagcag tttcgcccga tacacagcaa tttgagaacc attttcttca agttctctaa
     1021 aatactttgt atgatgaaac acccataaca aagtagcagc tttttcagca ttatccatag
     1081 acggtaaaga tagctgaata tacaaatatc gcgatccatt taaggaagtt acatctacct
     1141 gagctgatac attttctaat ttataatcac tgagcaacat ttctacttct ctaatcgcca
     1201 gtgtattaac tgaattatcg gatatatttg tctgaattct attcagtaca gaatttatct
     1261 gttctacatc gataattctt tcttctccaa aaagttgatt tcctttatta tcttgaatga
     1321 tcgttctgta actctgaata gaataacctt tctcacgggc agaaagggcg actattctat
     1381 ctactgagtt aagagtaaga tgatcctcaa aggttattcc ttcgttttgg aagctgactg
     1441 caacaacttg aattttcaaa gggttctctt ctaaaacaac aacttgctta accggaacgc
     1501 tttgcagctt aaattcctca atcaagtacg taacaacatc tacctgagcg ccagccttta
     1561 caaaaaagcc aaacgctatt cccacaacag ctatcagtaa aaaaatcttc aaatactgtc
     1621 ttttgttttt catgcctacc tccaatcaac ttctactacg gcacaggccc gtaggtgcga
     1681 aatgaagaat tttgagattt attccaaata agatatgggt aatcttcaac ccacgaaggt
     1741 tgatcgaaca aaagccaatt accattgaca tcccttacag ctattgaatt gctggcaaag
     1801 ttgggtaagg cacttccgtt aaagccgggg ctcgtgtggg caacacagga aattcctcgt
     1861 ttgtcttctg caaaactggc aaattggtta caggcaattc catttgtggg gagggcagga
     1921 tctctcgcat gatttctcca gtttcgccat ctagaaatat gatcatttgc ttatgaataa
     1981 tctccgttgc tggaatgtca ccacctgccg acgcgggtac atgctctaca aactcccctt
     2041 caagcacgat caaccatatg gttttattcc gcttctcgta ttcttttgtg tttggctcaa
     2101 cagatttccc gaaaacaaac agcatggctt cgtcataggt catcacttga ccataggctt
     2161 tctctggcgg caccaaccag ccacccaaac gagtatcttt agaataagaa aacgcaaggt
     2221 ctaatgcaag actctctgaa acattagtca tcggttctgc ctcggcacct ttcactttta
     2281 agcaggatgt caaaatcaga gcaattgtca aaattacagg caggattttc tttttcatat
     2341 cttcaatcgg gccttttcag caaatcaacg gcttgttcaa tttgcagctc aagttctttg
     2401 atcgttgaag ttacaaaaac cgcatccgtt ccagagtttt gaccgtacga aaaaaccgct
     2461 tcaccgtcaa ttccatagcg tccaaatggg tttacgattc gataaagttc acgatctgga
     2521 gcgtgaattt ggtcaccaga gatgttcact aaaaacaaga tatacccctc tcttactttg
     2581 tacagaggat catctccaac ctcttcaatt ccctttgaca cagttgggtc atacccgcca
     2641 atttgcatga cagtgatttg cgatggtgga tcacctagat acgtctttac tacttcaaag
     2701 actgtgtccg agaaaagcgc ttcgaacgtt tctgaaccaa ctattttatt attttcaccc
     2761 caaaccggaa gttcatgacg aagcactcga gtaacaggcg cctcaataac ccgtactctc
     2821 acgataaggt ctgcctctga cacaagttct tccacagttg ccgctttgga agcccattcg
     2881 acagtagagc tcccgcctgt aaaaacaggt gctacttcgg tatctttcgc ttttgcacat
     2941 gaagtcaaaa ccaaggcaag cgtcaagatc gcaaggatcg tttttttcat aatttgcaac
     3001 ctttctttgg ttgggggaac aaaatcaatt caccccatac aatggattat attgacgtgt
     3061 ttcatttcgt gcaagcggac gattttcaaa agacaattca tatctacaaa attttccacc
     3121 tttgcaaact gccccaccgc gaatacaatc accccgccat gaaaacaact ccacttttcg
     3181 atgaagcagg catggaatac cagaagaccc gcatcgccca ctgggacaag gtcgcgaaga
     3241 aacgcgatgc ctgggccg
//
LOCUS       DAOQJE010000355         3252 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_19314_length_3252_cov_7.203316, whole genome shotgun sequence.
ACCESSION   DAOQJE010000355 DAOQJE010000000
VERSION     DAOQJE010000355.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3252)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3252)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3252
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19314_length_3252_cov_7.203316"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..399
                     /locus_tag="PLT08_10005"
     CDS             <1..399
                     /locus_tag="PLT08_10005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HNE04841.1"
                     /translation="LMELLNEMYKAREAGAAGSDEWKEAQEIYLKMMAPVTPHIAEEL
                     WTEHLGKPYSIHQQLWPVVDEVAAKDDFVEIPVQINGKVRDRVKVAAEASEEEIKSAV
                     MASEVIQKFLEGKEPKKVIVVKGKLVSLVV"
     gene            597..>3252
                     /locus_tag="PLT08_10010"
     CDS             597..>3252
                     /locus_tag="PLT08_10010"
                     /inference="COORDINATES: protein motif:HMM:NF019136.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component regulator propeller
                     domain-containing protein"
                     /protein_id="HNE04842.1"
                     /translation="MIRQWMAHRELNKTVLIALMLLALGSGLLPTPALAQMVKTKKNI
                     QPLNLPIAPDFKFTHLSIEDGLSQSTVTTILQDRHGFMWFGTQDGLNRYDGYSFVVYR
                     NDPNDLNSLGGNQIRKIVQDASGILWIGTSGGGLNKYNPETDTFVRFINEPENPNSLK
                     NNVIWNILADETGILWLYMDDGWLTKFDPKTGTFTHYHSDVTAPFNAEVAYYESKALY
                     QDKNGDLWLSNYKGGLEKFDPITEQFTFFFNDPETPSTISAGGVTNVYEDDAGHFWIC
                     TSDGGLNLMDRKSGTFKHFQHDPANPYSLSDNNCRQILQDHMGLYWVTTGRGLNLFDP
                     ETGRFSRYHKDPSNPYSLSDDNLSAIYEDQGGVLWFGTYGGLNKLNLSTVQFEHYRHI
                     PENPDSLSTSYIYSIYEDKDGILWVGGDDGVLNRFDRHNNHVIHYEPNADDPNALNKS
                     WSVSALYEDHTGTFWVGTFSGGLHTFDRKTGKFQRYLHNPDDPDSISSDIILAICEDS
                     NGNIWVGTEGGGLNRFDRATATFQHYLPTSNDPKDKRPSSVRGIYEDKAGFLWLTSWY
                     EGLTLFDPRTEQFVNYRHDSENIESLGNDLTYVVHEDQMGTLWVGTDSGLDRFDRITN
                     TFYHYTVQHGLPNNVIYAIMEDTSGNLWVSTNRGISKFDLRTITFHNYNVFDGLQSDE
                     FNQNAYFQSSSGEMFFGGVNGLNAFYPDQVTSDPYRPPVILTDLQLFNRSVSVSEKSV
                     LTKPIWDTEQVTLNYDQDVFSIAFAALSYAAPAENLYRYKLEGFDEDWNNVDAKRRFA
                     MYTNISPGEYTFRVQGSNKDGVWNEEGVAIKIIITPPWWDTWWFKSGVLITIFTLVFG
                     IYLIRVKSLERSNRELSFAYEATIEGWSR"
BASE COUNT          966 a          682 c          724 g          880 t
ORIGIN      
        1 ttgatggaat tgctgaacga gatgtacaaa gcccgtgaag ccggtgccgc tggttcagat
       61 gaatggaaag aagcgcagga aatctacttg aagatgatgg cgcctgtcac tccgcacatt
      121 gccgaagaac tgtggacgga acacctgggc aaaccctact ccattcacca gcagctgtgg
      181 cccgttgtag acgaagttgc tgctaaagat gatttcgtcg agatccccgt ccagatcaat
      241 ggcaaggtac gcgaccgtgt caaagtcgct gccgaagcca gcgaagagga gatcaagtcg
      301 gcagtcatgg caagcgaagt catccagaaa ttcctggaag gcaaagagcc gaagaaagtt
      361 atcgtggtga agggcaagtt ggtaagtttg gtggtgtaaa aagcgcttcc ttcactgtta
      421 tagctcaact caaaagccct ctgaactagt tcagagggct tttgagttag tgccaaatgt
      481 cagtagtaaa agaatatcaa atatgtcaaa atacgaactt aataatactt gcacactttt
      541 ggcagaagtg gtgtttattt attattgctc aaccgtattt taggagaagt aatattatga
      601 tccgccaatg gatggctcac cgtgaactta ataaaaccgt cctgattgcc ctcatgcttc
      661 tggcgctggg atctggctta ttgccaacgc cagcactcgc tcaaatggtc aagacgaaaa
      721 aaaatattca gcctcttaat ctacctattg caccagattt caagttcact catttatcta
      781 ttgaagatgg attatcccaa tcaaccgtaa ccaccatctt gcaagatcgc catggattta
      841 tgtggttcgg gacacaagat ggtttgaatc gttatgatgg ttatagtttt gttgtatatc
      901 gtaacgaccc taatgacctt aatagcttgg ggggcaacca gattcgcaaa attgttcagg
      961 atgcttcagg cattttatgg atcgggacat cgggaggcgg gttaaataaa tacaatcccg
     1021 aaactgatac ctttgttcgt tttataaatg agccagaaaa ccccaatagt ttgaagaata
     1081 acgtcatatg gaatatcctg gcagatgaaa ccggtatatt gtggttatac atggatgatg
     1141 gttggttgac caaattcgat cccaagactg gaacatttac tcattatcat tctgatgtta
     1201 cggcgccatt taatgctgaa gttgcttatt atgaaagcaa agctttgtat caagataaaa
     1261 atggcgacct gtggttaagt aattacaaag gtgggcttga aaaatttgac ccaatcacag
     1321 agcagtttac ttttttcttt aacgacccag aaactccaag cacaatcagt gcaggcggag
     1381 tgaccaatgt atacgaagat gatgcggggc atttctggat ttgcaccagc gatggtgggt
     1441 tgaacctaat ggatcgcaaa agtggcacat ttaaacactt tcaacatgac cctgccaacc
     1501 catatagtct tagtgataat aactgtcgtc aaattttaca ggaccatatg gggttatatt
     1561 gggtcactac gggacgcggg ctaaatctat ttgatccaga gaccggtaga ttcagccgct
     1621 atcacaaaga tccaagcaat ccgtattctt tgtctgacga taatctttct gccatttatg
     1681 aagaccaagg cggcgtgctc tggtttggaa cctatggcgg gctcaataaa ctcaacctca
     1741 gtactgttca atttgagcat tacaggcata ttccagagaa cccagatagt ttaagcacaa
     1801 gctatatcta ttcgatttat gaagataaag atggtatcct ctgggtaggt ggagatgacg
     1861 gtgtattaaa ccgttttgac cgacacaata accatgtcat ccattatgaa ccgaatgcag
     1921 atgatcccaa tgcattaaac aaaagctggt ctgttagtgc actttacgag gatcataccg
     1981 gcacattttg ggtagggaca ttctctggag ggttacacac atttgaccgt aaaacaggaa
     2041 aatttcaacg ttaccttcac aacccagatg atcccgatag tatcagcagt gacatcatcc
     2101 ttgcaatttg cgaagatagc aatggcaaca tctgggtcgg aacagaaggg ggcggattaa
     2161 atcgattcga ccgtgcaact gctacttttc aacattacct acccacttca aatgacccaa
     2221 aggacaaacg tcccagcagt gtgagaggca tatatgaaga taaggcaggt ttcttatggc
     2281 taaccagttg gtatgaagga ctgactctat ttgatccgcg cacggaacag tttgtaaatt
     2341 atcgacatga ctcagaaaac atagaaagcc tgggcaatga cctaacttat gttgtccatg
     2401 aagaccagat gggcacacta tgggtaggca cagacagtgg actggatcgt tttgaccgta
     2461 taacaaacac tttctatcat tacactgtgc aacacggact accaaacaat gtaatttatg
     2521 ccattatgga agatacttct ggtaatttat gggtttctac caatcgcggt atctctaaat
     2581 ttgacctacg tacaatcacc tttcataact acaacgtctt tgatggactg caaagtgatg
     2641 aatttaatca aaacgcctac ttccaaagtt ccagcggtga gatgtttttt ggcggtgtga
     2701 atgggctgaa tgcattctat cccgatcaag tcacaagcga tccgtatcgt ccgccagtga
     2761 ttctgaccga cttgcaactc tttaaccgct ccgtctccgt tagcgaaaaa tcagtcctca
     2821 caaagccaat ttgggataca gaacaagtta ctctaaacta tgatcaagat gttttttcca
     2881 ttgcatttgc tgcactcagt tacgccgccc ctgctgagaa cttataccgc tataagttag
     2941 aagggttcga tgaagactgg aacaatgtag atgccaaacg gcgttttgcc atgtacacaa
     3001 atatttctcc tggggagtat acctttcgcg tacaaggatc aaataaagat ggagtatgga
     3061 acgaagaggg agttgcgata aaaatcatta tcaccccacc atggtgggac acctggtggt
     3121 ttaagagtgg ggttcttatt actattttca ctctagtttt tggcatctac cttatacgtg
     3181 tgaaaagcct tgaacgatct aaccgtgaat tatcatttgc atatgaagcc accatcgaag
     3241 gttggtctcg tg
//
LOCUS       DAOQJE010000356         3250 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_19338_length_3250_cov_5.619718, whole genome shotgun sequence.
ACCESSION   DAOQJE010000356 DAOQJE010000000
VERSION     DAOQJE010000356.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3250)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3250)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3250
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19338_length_3250_cov_5.619718"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..206)
                     /locus_tag="PLT08_10015"
     CDS             complement(<1..206)
                     /locus_tag="PLT08_10015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE04843.1"
                     /translation="MKPRLMFWTLGLVIALLAIACAPSNPTETAPPVVEATATVASAP
                     LVETAVPQVEATIPAVLPVATSRGP"
     gene            439..2142
                     /locus_tag="PLT08_10020"
     CDS             439..2142
                     /locus_tag="PLT08_10020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HNE04844.1"
                     /translation="MANFKLTYATMFNPPEELHTGFDKAVAKLKENMGKEYGMFINGK
                     EVFADEKFEDRSPINTDWVLAKMQKGNASHAQMAVDAARKAFPMWSRTPWQQRVKLLR
                     KAASLIEDRIFELGAAMAMEVGKNRMESMGDVQETADLIYYSCYQMEKNNGYIAEMGR
                     DPLVGYESRNVSVLRPYGVWLVVSPFNFPHALTGGPSGAALAAGNTLVIKPATDTAWI
                     VRLLVDCFRDAGFPEGVVNFVTGPGSTLGQALVDSPEVDGVTFTGSFDVGMKMYRDYA
                     NRNYVRPMILELGGKNPAIVSRNADLETAAVGIVRSAFGLQGQKCSAASRVLVEEPVY
                     DELVARLKDLTDKLVLGDPTNRATYNGPVINKSSYQDFKDFTEEINQGGGKFLTGGHV
                     RVDGDYGKGFFCESTFVTDLPFEHRLWKHEMFLPITTIGKVQNLDQAMGIANDVNYGL
                     TAGFYGNPAETEWFFDNIEAGVTYANRPQGASTGAWPGFQPFGGWKGSGSSGKNAGGH
                     YYLPLYMHEQIHTSVKVAKAAPAKKAAAKKTVAKKVATKKVAAKKTVKKAVKRIAKKK
                     K"
     gene            2196..2588
                     /locus_tag="PLT08_10025"
     CDS             2196..2588
                     /locus_tag="PLT08_10025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE04845.1"
                     /translation="MRVIKQSFQIIHYLIFMVGILFISACSNELNTPTPVATENVPTF
                     NVQIPDKALLKNYLVVNLETEPNTTCELTYIAPKGQKLHMIETADKNGICEWRWKLEE
                     SDGRGHGRLIFTINGVSETHFIDIRPGF"
     gene            2659..>3250
                     /locus_tag="PLT08_10030"
     CDS             2659..>3250
                     /locus_tag="PLT08_10030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE04846.1"
                     /translation="MEQELKLRDYPIIGWLVGLGGLGWGGYTFFNTLPAFNLNAALVG
                     FGGLLFLVLSYSLVVTADKTTGLLILDYRSIFHHSTKEIPLSQISNIRVDRSRTRSSK
                     GGTSTTYRVEAILSSGEVVPFRSYHTGNLFKHQKWANELRAFLNISEGFDETPQGFFK
                     AAQQAGAMIAQRQQEALTGSNAAMRNTDGVNWQLQSF"
BASE COUNT          876 a          816 c          792 g          766 t
ORIGIN      
        1 ggaccacgag acgtggcgac cggcaaaaca gcaggaatag tcgcctccac ctgggggacg
       61 gctgtttcca cgagcggagc ggatgccacg gtggcggtcg cttctacaac gggcggagcg
      121 gtttcagtag ggttggacgg ggcgcaggca attgccagaa gcgcaattac caagcctaag
      181 gtccagaaca tgaggcgggg tttcatcagt tttctcctta ttaaagttaa ttaaatgata
      241 ccaaggcact gcatcctctt acaaaattca cacaaacttc tcatgaggta tgtcacaggc
      301 aaaacagtcg ctttactttg acgacggtta aatactacgc tatacttaat ttgcactttg
      361 tctcacaata gggtcacttg ctcagggtga cacgagataa tttgcagatt tgtctcacac
      421 ttctaaagga gaactcctat ggcaaatttc aagctcacgt acgcaaccat gttcaaccca
      481 cctgaagaat tgcacaccgg cttcgacaaa gccgttgcca aacttaagga aaatatgggc
      541 aaggaatatg gcatgttcat caacggtaag gaagtttttg ccgatgaaaa gttcgaagac
      601 cgctcgccca tcaacaccga ttgggtgctc gccaaaatgc aaaaaggcaa cgccagccac
      661 gctcaaatgg cagtggatgc ggcgcgcaaa gccttcccga tgtggagccg cactccctgg
      721 cagcaacgtg taaaactgct ccgcaaggct gcttctttga tcgaagaccg catctttgaa
      781 ctcggcgccg ccatggcaat ggaagttggc aagaaccgca tggaatctat gggcgatgtg
      841 caggaaactg ccgaccttat ttattattcc tgctaccaaa tggaaaagaa caacggctac
      901 attgcagaaa tgggacgcga cccgttggtg gggtacgaat ctcgtaacgt gtctgtatta
      961 cgcccttatg gtgtgtggct cgtggtatca ccgttcaact tcccgcacgc gctcacaggc
     1021 ggaccttcgg gtgctgctct ggctgcgggc aacacccttg tcatcaaacc tgcaactgat
     1081 acggcttgga ttgtgcgctt gctagtagat tgcttccgtg atgcaggctt ccctgaaggt
     1141 gtggtcaact ttgtgacggg tcctggctcg actctgggtc aggcactggt cgacagcccc
     1201 gaagtcgacg gcgtcacctt taccggttct tttgatgtcg gtatgaagat gtatcgtgat
     1261 tatgccaacc gcaattatgt gcgcccaatg atcctcgagc tgggcggtaa gaatcctgcc
     1321 atcgtttccc gcaatgcaga ccttgaaact gcggctgtgg ggattgtccg ctcggcattt
     1381 ggcttgcagg gacagaaatg ttcggcggcg tctcgcgtgt tggtggaaga acccgtctat
     1441 gatgaactgg ttgcccgcct caaagacctg accgataaac tcgtgctcgg tgacccaacc
     1501 aaccgcgcca cctataacgg acctgtgatc aacaagagtt catatcagga tttcaaagat
     1561 ttcactgaag agatcaacca gggcggcggc aaattcctaa caggtggtca cgtccgcgtg
     1621 gatggcgact acggcaaagg cttcttctgc gaatccacat tcgtgaccga cctgccgttt
     1681 gaacatcgcc tgtggaaaca tgaaatgttc ctacctatca ccaccatcgg caaggtacaa
     1741 aacctcgacc aagccatggg catcgccaac gatgtcaact atggtctgac cgccggtttc
     1801 tacggcaacc cagccgaaac tgaatggttc ttcgacaata tcgaagctgg cgtgacctac
     1861 gccaaccgcc cacaaggtgc ctccacgggt gcctggcctg gcttccaacc cttcggtggc
     1921 tggaaaggct ctggctcatc tggtaagaat gcaggcggtc actactatct gccgctctac
     1981 atgcatgagc agattcacac ttcagtaaaa gttgcgaaag cggctcccgc aaagaaggca
     2041 gcagcgaaga aaactgtagc aaagaaagtg gctacaaaaa aagtagctgc aaagaaaacg
     2101 gtcaagaaag ccgtaaagag gatagctaag aaaaagaagt aatctgaaat tagcctctcg
     2161 agtacgctcg ggaggctttt tgatagaatt ctcctatgag agtcataaaa caaagttttc
     2221 aaatcattca ctatcttatt tttatggttg gtatattgtt catctccgct tgtagcaatg
     2281 aacttaacac accgactcct gtagccacag agaatgtgcc gacctttaac gtccaaatcc
     2341 cagataaagc tctcttaaaa aattatctcg ttgtcaattt ggaaacagag ccaaacacaa
     2401 cctgtgagtt gacctacatt gcccccaaag gacaaaaact tcacatgatt gaaaccgctg
     2461 ataaaaacgg aatctgtgaa tggcgctgga agcttgaaga aagcgatggc agagggcatg
     2521 gacgtctgat tttcaccatc aacggcgtca gcgaaaccca ctttatcgac atccgcccgg
     2581 ggttttagat aggagctttg tctggtttgt atccattgaa aatttcttat aattaaataa
     2641 aaacagagga gcaacataat ggaacaggaa ctcaagctta gagattatcc gatcattggt
     2701 tggctggtag ggcttggtgg actcggttgg ggtggctata cctttttcaa tacattacct
     2761 gctttcaacc tgaatgccgc cctagtgggg tttggagggc tacttttcct agtcttaagt
     2821 tactcactcg ttgtcactgc cgataagacc accggcttac tcatcctcga ctatcgctct
     2881 atttttcatc attccactaa agaaattccg ctcagtcaga ttagtaatat cagagtagac
     2941 cgttctcgca cacgcagcag caaaggagga acaagcacaa cctatcgtgt tgaagcgatt
     3001 ctatcaagtg gtgaagtcgt tccattccgt tcgtatcaca ccggaaattt attcaagcat
     3061 caaaaatggg caaatgaatt gcgggcattt ctcaatattt ccgaagggtt cgacgaaact
     3121 ccgcaaggct ttttcaaggc agcacaacaa gctggagcca tgatcgcaca gcgtcaacaa
     3181 gaggcgctca caggctccaa tgccgccatg cgcaacacag acggtgtcaa ctggcagtta
     3241 cagtcctttg
//
LOCUS       DAOQJE010000357         3230 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_19531_length_3230_cov_5.772598, whole genome shotgun sequence.
ACCESSION   DAOQJE010000357 DAOQJE010000000
VERSION     DAOQJE010000357.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3230)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3230)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3230
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19531_length_3230_cov_5.772598"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            108..920
                     /locus_tag="PLT08_10035"
                     /pseudo
     CDS             108..920
                     /locus_tag="PLT08_10035"
                     /inference="COORDINATES: protein motif:HMM:TIGR03696.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
     gene            922..1944
                     /locus_tag="PLT08_10040"
     CDS             922..1944
                     /locus_tag="PLT08_10040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE04847.1"
                     /translation="MNIKKTFLSLVLFICVGCNNLVSGTSYSSAKQLPFPTERGYSRI
                     NLIDDQIVVFDREGGRLYYSIENNEQFEQIPDKRMDFCPDDDFYKFLSYSPFVTVLPD
                     QRIGIIGACFHNVDYMFAYDIETGKMEKIVEEQLLVPANNFSWNPTMTMGVQDSYTQL
                     DGTIFWLNSEEASAMDITLSDGNRSWSLAMDFPKIVGDGRQGIAAYPVWSPDGKQIAF
                     FATFDSIGKSGFSILDSEYAIYTMDSDTLVPTQILNGIYTDYAITLAWSPDGEYISFT
                     GKYGFPKQYGLWVLNLESKNVYFIDNNITGSVWRNDDESIIGIKCIGNSCEKQEVWEY
                     WLKELK"
     gene            1951..2154
                     /locus_tag="PLT08_10045"
     CDS             1951..2154
                     /locus_tag="PLT08_10045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE04848.1"
                     /translation="MYYLLAGHLGSTNPMTDTSGAKISEMRYKPFGEVRYTWKASQTT
                     TPAYALSRFTFTGQYSHSPFGGP"
     gene            2154..3155
                     /locus_tag="PLT08_10050"
     CDS             2154..3155
                     /locus_tag="PLT08_10050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE04849.1"
                     /translation="MKTKVSYLLCLTLLICSCAPNANQDLNISKNGPIKGLVNYKFSS
                     PAWITDQTIAFIGKPIMTDRKSRSSLLLYNTQTSTWKVVDFPISDRCDVEGYTFLSRL
                     PNNNLGAVKICVKGISETRTIVEFSVNGENEKILVNRTSTIGILGKFAFSPDMGKVVV
                     EDMTNRFLGNKIYLYTNENFINIQNSFTRVMYPDWSGTINKIAFWGTENYPGGSPETL
                     STLPEIMSLSSYPWDLYISNSDGTQTIKLLTGVVDALQIKWSPADHLIAFSGTVNGQS
                     GLFILDANIPEHPYVVWENLVDFDWSPNGKNIILIDNKQNDIVMYILGLESLFLQKE"
BASE COUNT          963 a          596 c          754 g          917 t
ORIGIN      
        1 atatcatccc cgtagggggt atggatgatc ccagcacaag cggagtcacc gagggctttg
       61 ggttgatgtt ctagtgcgaa gtctcccgta gggataatgt ccgctggtat gacccagcgt
      121 tgggacgttt tgcccaagcg gacacgattg tcccgctggg tgtgcagggg ttggacagat
      181 acgcgtatgt caacaactcg ccggtcaatt ttgtcgaccc gacggggcat tgtggtatcg
      241 aatatgacga taatggtaat ccaactgttg gagatctaga ttgcactgcc aatgacatca
      301 aaaaatggtc tataaaatac aggcaacagt ggttcgatct tcttactgaa cactatggtg
      361 attggttcaa caatatcgag ggaattttac aagcctttaa tgacataggc attgaaagcg
      421 gggatacctg ggcatctgtt actgatgccg gaattctgga aacaatccag aacggcattg
      481 cagccacaga gtatggcata agcctagatt cctttggaga gcaagggagt ctacggcggg
      541 acgcctcgga aaaatgggct gcattctttc aaatcgtttc tgaccctgaa gtttctgttg
      601 cgacgaagaa aactctttgg ggaagtgcgg aggtggcggg gacggactac ggaaaagacc
      661 ttgcagaaga cgggctggga ttaagtccaa ataaaaacga acgagatttt ctgttgattg
      721 gcgatggata tcgtacggtg atgggagtac cttatggcgg cgagatactg ggatttgggt
      781 ccggttattt aggctgtggc ataggatgtg ggataacagg ttggcaatta ggtggatggg
      841 caactgaccc aagaagtcaa gatcctattt tcgggcaagc tccagtctat tatgtagctg
      901 tatttgtatt gggacgttaa catgaatatc aaaaaaactt ttcttagcct tgtgttattt
      961 atttgcgttg gatgcaataa ccttgtgtct ggaacatcct actccagcgc aaaacaattg
     1021 ccatttccta cagagagggg gtactctaga atcaacctga ttgacgatca gattgttgta
     1081 tttgatcggg aaggaggaag attgtactat tccatcgaaa ataacgagca gtttgagcaa
     1141 ataccagata aaagaatgga tttctgtcca gacgatgatt tttacaagtt ccttagctat
     1201 agcccgtttg taactgtact gccagatcaa aggattggaa taattggtgc ctgttttcat
     1261 aatgtggatt atatgttcgc ctatgatatc gaaactggga aaatggaaaa aatagtcgag
     1321 gaacaattgc tggttcctgc caacaacttt tcctggaatc cgaccatgac catgggcgtt
     1381 caggattctt atacccaatt agatggcaca atattctggc tgaattctga agaggcaagt
     1441 gcaatggata tcaccttatc ggatggaaat cgttcctggt cgttggcgat ggacttccct
     1501 aagatcgttg gggatgggag gcagggcatt gcggcttacc ctgtctggtc tcctgatgga
     1561 aaacaaatcg cttttttcgc tacgtttgat tccatcggaa aatctggatt ctcaatcctg
     1621 gatagtgagt atgctattta cacaatggat tccgacacac ttgttcctac tcaaattcta
     1681 aatggaatat acacagatta cgcaattact ctggcatggt cgcccgatgg ggaatatata
     1741 agttttacag gcaagtacgg ctttccaaaa caatatggtt tgtgggtctt gaatctagaa
     1801 agtaaaaatg tttattttat tgataacaac ataacaggca gtgtttggag aaacgacgac
     1861 gagtcaatta taggaattaa atgtattggt aattcttgcg agaaacaaga agtgtgggag
     1921 tactggttaa aggagttaaa gtgaggtgta ttgtattact tgcttgctgg tcacctcggc
     1981 tcgaccaatc ctatgaccga tacaagcggc gcaaagatct cggaaatgag atacaaacca
     2041 tttggcgaag tgcgctatac atggaaggcg agtcagacca ccacaccggc gtatgccctg
     2101 tcccgcttta cgttcacggg acaatattcc catagtccat ttggaggacc ttaatgaaaa
     2161 caaaagtgag ttatttactt tgtttgacct tattgatttg cagttgcgca cctaatgcaa
     2221 atcaggatct aaatatctcg aaaaacggac caattaaagg gcttgttaat tacaaattca
     2281 gttcgcctgc atggattaca gatcaaacaa tagcatttat tggtaaacct ataatgacag
     2341 atcgaaaaag tcgctcttca ttgcttctct ataacaccca aacatctact tggaaagtgg
     2401 ttgattttcc gattagtgat agatgcgatg ttgaaggata tactttctta agccgattac
     2461 ctaataataa tttaggagcg gtaaaaatat gcgttaaggg aatatcagaa actagaacga
     2521 tcgttgaatt ctcggtgaat ggcgaaaatg aaaagatatt ggtaaataga acctctacta
     2581 tagggatatt agggaaattc gcattttcgc cggatatggg caaagttgtc gttgaagata
     2641 tgacaaatcg ttttttagga aataaaatat atctttatac aaatgaaaat tttatcaata
     2701 tacaaaattc tttcactaga gtaatgtatc ccgattggtc cggcacaatc aacaaaattg
     2761 cattctgggg aactgaaaat tatccgggcg gcagtcctga aaccctgtct actttacctg
     2821 aaataatgtc acttagttct tatccatggg atttgtatat atctaactct gatggcacac
     2881 aaactattaa acttttaact ggagtagttg atgccttaca aattaaatgg tcgcctgcgg
     2941 atcacctcat tgcattttca ggaacagtta atgggcagtc tggtcttttt attttagacg
     3001 ctaatatacc ggaacatccc tacgtggttt gggaaaactt agttgatttt gattggtcac
     3061 caaacggtaa aaatataata ttaattgaca ataaacagaa cgatattgtc atgtatattc
     3121 ttggcttaga atcattgttt ttgcaaaaag aataaagaaa acatgccaac tgatagatcg
     3181 cccagcgcaa gtacaccatc ccgcagagca tgagcgtgga atacttcctg
//
LOCUS       DAOQJE010000358         3216 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_19668_length_3216_cov_6.780766, whole genome shotgun sequence.
ACCESSION   DAOQJE010000358 DAOQJE010000000
VERSION     DAOQJE010000358.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3216)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3216)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3216
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19668_length_3216_cov_6.780766"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..192
                     /locus_tag="PLT08_10055"
     CDS             <1..192
                     /locus_tag="PLT08_10055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE04850.1"
                     /translation="VQVGKGLNPQEWIQLGEDNYTPVEGGILAEWDTTGLSGLYAVQL
                     IVVRQDQKVDTAVIQVTVK"
     gene            complement(233..2113)
                     /locus_tag="PLT08_10060"
     CDS             complement(233..2113)
                     /locus_tag="PLT08_10060"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family oligoendopeptidase"
                     /protein_id="HNE04851.1"
                     /translation="MAYKLSKWDLSQLYSGYDSPELENAFDMIEEQVASFEGVRSKLT
                     PDISAEQFMQVMKADELSTRVANKLYSFAGLSFTADTQDQKAQALQARVQQFLAENQN
                     RILFFSLWWKELDDANAKRLMDASGDYRYYLESMRNFKPHTLSEAEEKVINLKNVNGV
                     NALGNLYDSITNRYVFKMKVGGKEKEMTAAELFSYRYSTDPAVRAASYQAQFKVYAED
                     GPILGQIYQTILRDWHSENIGLRKFKNSIAPRNLSNDVPDEAVDALLNVARKNTGIFQ
                     RYFTMKAKHLGMKKIRRYDIYAPLAASKKTYDWDTAVHMVLDAFNNFSPKVAELAQRV
                     FDQNRIDSEIRKGKRGGAFCWGFLPEETPYVLVNYQGNSREVATLAHELGHAIHAMLA
                     EQHSVYTFHSSLPLAETASTFAEMVLIDKLLTEETDESVRRDILFKQMDDAYATVLRQ
                     SYFALFEKTAHEMTQKNASIDDLSAAYLENLKEQFGDSLELSDEFKWEWVGIPHIYQT
                     PFYVYAYAFGQLLVYALYQQFKQEGESFKPKYLKILSAGGSEAPERILSEAGVNIRDP
                     KFWQGGFDFLERLVSELEKLPVEKAKKPAAKKVTKKAVKKVAAKKPAKKAPVKKVAKK
                     KK"
     gene            complement(2210..>3216)
                     /locus_tag="PLT08_10065"
     CDS             complement(2210..>3216)
                     /locus_tag="PLT08_10065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF933 domain-containing protein"
                     /protein_id="HNE04852.1"
                     /translation="ALTRGNAPTNASAGRIEVHQSVVDVPDPRVDLLSKMFNPRKTIY
                     TKITYADIAGLDSGSAKSGISGQLLNHLNQMDALILVVRAFENDNVAHPNGSINPLRD
                     VDTMTSELLLNDLIAVERKLERLTDERKKGGTDKTLNARQTELFEKMHKALSDNKPLR
                     DLEYTPEEEKELSSFGLLTRKRLLTVFNLGEGQSAPDAKLDHPSVALMGKLEMEIAQL
                     SAEDAAVFMDEYGIKELSLNRMINLSYELLQELTFFTVGEDEVRAWVTHRGATAQEAA
                     GEIHTDLSRGFVRAEVVAYEDLISLGSMNEAKAKGKFRLEGKEYIVKEGDIMHVRSSL
                     "
BASE COUNT          754 a          784 c          789 g          889 t
ORIGIN      
        1 gttcaagtgg ggaaggggct gaatccgcaa gagtggattc aattgggcga agataattac
       61 accccggtag aaggcggcat cttagctgaa tgggatacaa ccggtctaag cggtctgtat
      121 gccgttcagt tgattgttgt ccggcaggat caaaaagtag atacagccgt tattcaagtg
      181 acagtgaaat agttcaaata aaaagacccg gctcattacc gggtcttttt atttacttct
      241 ttttcttcgc aaccttcttt acaggtgctt tcttggcagg ctttttcgct gccactttct
      301 ttacagcctt cttggtgact ttctttgccg caggcttttt ggctttttcc actggcagtt
      361 tttccaactc actcaccaaa cgctcaagga agtcgaaacc accttgccag aatttcgggt
      421 cacgaatgtt gacaccggct tcgctcaaaa tgcgttctgg cgcttctgaa ccgcccgccg
      481 aaaggatctt gaggtacttc ggcttgaagg attcaccttc ctgcttgaat tgctgataca
      541 aggcgtacac cagcaattga ccgaaagcat aggcatagac gtagaacgga gtctgataga
      601 tgtgcggaat gccgacccat tcccatttga attcgtcgct cagttcgagc gagtcaccaa
      661 attgttcttt gaggttttca aggtacgcag cgctcaagtc atcgattgag gcgttctttt
      721 gagtcatctc atgcgcggtc ttttcaaaca gcgcaaagta cgattgacgc agcacggtcg
      781 cataggcatc atccatttgt ttgaagagaa tgtctcggcg aaccgattca tctgtttctt
      841 ccgtgagcaa tttgtcgatc aagaccattt cagcaaaggt cgaggcggtt tcagccaacg
      901 gtagtgacga atggaaggtg tagacactgt gctgttctgc cagcatggca tgaatcgcat
      961 gacccagttc gtgcgcaagg gtggcaactt cacggctatt gccctgatag ttcaccaaca
     1021 catagggagt ctcttcgggt aaaaagcccc aacagaaagc gccgccgcgt ttgcctttgc
     1081 ggatctcgct atcaatgcga ttctggtcaa agacgcgctg ggcgagttct gccactttgg
     1141 gcgagaaatt attgaatgca tccaacacca tgtgaacggc agtgtcccaa tcataagttt
     1201 tcttggaagc agccaacgga gcatagatgt catagcggcg gatcttcttc atgccaagat
     1261 gtttggcttt catggtgaag taccgttgga agatacctgt attcttgcgt gccacattga
     1321 gtaaggcatc caccgcttcg tcagggacgt cgttgcttaa gttgcgcggt gcaatggagt
     1381 tcttaaattt gcgcagccca atattctcac tgtgccaatc gcgcaagatg gtctggtaaa
     1441 tttgcccaag gatgggacca tcctctgcat ataccttgaa ttgcgcctgg tagcttgcag
     1501 cgcgaactgc cgggtctgtg ctgtagcggt aagagaacaa ctcggcagcg gtcatttctt
     1561 tttctttgcc gcccaccttc atcttgaaca catagcggtt cgtgatcgag tcgtacaggt
     1621 tacccaacgc attcaccccg ttgacgttct tcaagttgat gaccttttct tctgcttcgc
     1681 tcaaagtatg cggcttgaag ttacgcatgg attcgagata gtagcgataa tcgccagaag
     1741 catccatcaa acgtttggcg ttggcgtcat ccagttcttt ccaccacaag ctgaaaaaca
     1801 aaatgcggtt ctggttctct gctaaaaatt gttgaacacg ggcttgtaaa gcctgtgctt
     1861 tttggtcttg tgtatctgct gtgaaggata aacccgcaaa cgaataaagt ttgtttgcaa
     1921 cgcgggttga aagctcatcg gctttcatca cctgcataaa ttgttcggca gaaatatcgg
     1981 gcgtaagttt gctgcgcacg ccttcgaaag atgccacttg ttcttcgatc atatcgaatg
     2041 cattttcaag ctctgggctg tcgtagccag aataaagttg agaaaggtcc cacttggaaa
     2101 gtttatacgc catgttgtct ccttgtttta ttcgtagagg gacgagttgc cctcgtccct
     2161 acgttttatc acaacttaaa aaactttaac ccattctacg gtgtggattt tacaaactgg
     2221 accgcacgtg catgatatcg ccttctttga cgatgtattc tttgccttcg aggcggaact
     2281 tgccctttgc cttcgcttca ttcatcgacc cgaggctgat caaatcttca taagccacca
     2341 cctcggcgcg aacgaatcca cgcgaaagat cggtgtgaat ttctcccgcg gcttcctgtg
     2401 ctgtcgcgcc gcggtgggtg acccaggcgc gcacttcatc ttcacccaca gtgaagaagg
     2461 ttaactcttg caacaactca tacgagaggt tgatcatgcg gttgaggctc aattccttga
     2521 tgccatattc atccatgaac acggctgcat cttccgcaga gagctgtgca atctccattt
     2581 caagtttgcc catcaacgcc accgaaggat gatctaattt cgcatccggt gcagactgtc
     2641 cctcgccaag gttgaacacg gttaacaaac gtttgcgggt gagcaagccg aagctggaaa
     2701 gttctttttc ttcttcaggg gtgtattcca aatcgcgcaa tggtttgtta tcagaaagtg
     2761 ccttgtgcat tttctcaaat aactcggttt ggcgggcatt gagagttttg tctgtgccgc
     2821 cttttttgcg ttcatcggtg aggcgttcta atttccgttc aacggcaatg aggtcgttca
     2881 acaacaactc actggtcatc gtatccacat cgcgcaaggg attgatgctt ccgttcgggt
     2941 gcgcgacatt atcattctca aaagcacgaa cgaccagaat aagcgcgtcc atctgattga
     3001 ggtgattcaa caactgaccc gaaataccgc ttttggcaga gccgctatcc aaaccggcaa
     3061 tatcagcata tgtaatcttg gtgtagatcg tcttgcgtgg gttgaacatt ttggaaagaa
     3121 ggtccacacg cgggtcgggt acatccacta cagactgatg gacttcgatg cgtccagcag
     3181 atgcgttggt gggggcgttg ccacgggtga gggcgt
//
LOCUS       DAOQJE010000359         3209 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_19738_length_3209_cov_6.868738, whole genome shotgun sequence.
ACCESSION   DAOQJE010000359 DAOQJE010000000
VERSION     DAOQJE010000359.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3209)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3209)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3209
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19738_length_3209_cov_6.868738"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(137..805)
                     /locus_tag="PLT08_10070"
     CDS             complement(137..805)
                     /locus_tag="PLT08_10070"
                     /inference="COORDINATES: protein motif:HMM:NF013702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HNE04853.1"
                     /translation="MKRLIVWFIRVVIHLVGKVEATGYENVPEKGGFVIAVNHLGFLD
                     APMAYYALDNWNLFIPVAEKWEESAILRWLGKHLNAIFIDRFNPDLKAMREMIHRMEQ
                     GQTLVIAPEGTRARDEKMAQGKPGVAYLASKMGWTIVPVAISGTEDRIVIQNLKKLKK
                     SSIKLTAGKSFVLPPFPKENREEKLQEYTDEIMCRIAVMLPEHNRGYYAEHPRLKELL
                     VENK"
     gene            complement(893..1282)
                     /locus_tag="PLT08_10075"
     CDS             complement(893..1282)
                     /locus_tag="PLT08_10075"
                     /inference="COORDINATES: protein motif:HMM:NF018894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HNE04854.1"
                     /translation="MANEKRKLPRRNFSYYMRVLKKDTGELIGQIIDISTGGFKLESS
                     KPLPSNVQMELCIDQVNQISPRSYIVFSARTRWCDRDPYDPTIYNVGFQLVDMTPADY
                     DVFVTMFNTYGEKKQAHHKTNTDYIWG"
     gene            complement(1379..2527)
                     /locus_tag="PLT08_10080"
     CDS             complement(1379..2527)
                     /locus_tag="PLT08_10080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase domain-containing protein"
                     /protein_id="HNE04855.1"
                     /translation="MRKVAILGIGQTKVDEHWDKSLREIGGEAAFLAMQDAGMDKVDA
                     LYVGNMLSPMVSGQNQLGAFFSDWIGLWKQEAVKIEAACGSGSAAFRSALMAVASGDV
                     ESALVVGVEKMTDKAGKDVTAALATAADADYEVEQGISFVGINALVMRRYMHEFGWKH
                     EDFAPFSINAHANAMHNPYARLHEKVSVEKFEKSSMVATPINLLDASPVGDGAAAVII
                     VPADKVMRQPKVVVAASGAATDTIAVHSRKDPMFLNASYLSSQQAYKMAGITPEDIDV
                     FELHDAFSIMAALSLEATGFAERGQGVRLGLENQIGIKGKIPVCTRGGLKARGHPVGA
                     TGMYQIVEVAQQLRGECGPTQVDGATVGMAQNIGGSGATILTHILRRE"
     gene            2658..3095
                     /locus_tag="PLT08_10085"
     CDS             2658..3095
                     /locus_tag="PLT08_10085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE04856.1"
                     /translation="MQKYGFILILLIVLVIGCKGEEKSYASPTLSEEWTIKMTQSGGI
                     MGLRRSIQVASDGSYLIIDERAQTSVTGTLTEQQLSELTDLIANLKFISPDNRAICAD
                     CFVYDLEIESGGKKMILQVEDINLPDSGIEALVTFLRGILESA"
BASE COUNT          857 a          813 c          689 g          850 t
ORIGIN      
        1 agcgaatcca accgacccaa gtgattggat gccaacatgt atcccccttc tttgggcagt
       61 ttgctaatat cgccgttaac ttcaatatcc gcaatcagcg gtaaaacttt tctggcgcaa
      121 aagcgaagga aggcactcat ttattttcaa ccaataattc ttttaagcgc ggatgctctg
      181 cataatagcc gcgattatgt tccggtagca tcacggcaat gcgacacatg atctcgtctg
      241 tatattcctg caatttttct tcgcggttct cttttgggaa tggcggcaag acaaaagatt
      301 ttccagccgt gagtttaatg gatgacttct tcagtttttt gagattctga atcacaatgc
      361 ggtcttcggt gccgctgata gcaactggca caatcgtcca acccattttg gaggcaagat
      421 atgcaacacc cggtttgcct tgcgccatct tctcgtcgcg agcgcgagtt ccttcgggag
      481 caataacaag agtctgtccc tgttccatgc ggtggatcat ttcacgcatc gctttcaagt
      541 ccgggttgaa gcggtcaatg aagatggcat tgaggtgttt tcccaaccat cgtaggatgg
      601 cactctcctc ccatttttct gccaccggaa taaaaagatt ccaattatcc aaggcgtaat
      661 atgccatcgg cgcatctaaa aaacccaaat gatttacagc aatgacaaaa ccgccctttt
      721 caggaacgtt ctcataccca gtcgcttcca ctttgccaac aaggtgaata accactcgaa
      781 tgaaccagac gattaatctt ttcatggcgg caattttaca cggttttatt tctgggaagt
      841 ttaaacaaaa aagccatgtt tcgtaaaaca tggcttgaac gttcgctaaa gatcaacccc
      901 aaatgtaatc tgtattggtc ttatgatgag cctgtttctt ttctccatag gtattgaaca
      961 tagttacaaa gacatcgtaa tctgccgggg tcatatccac aagctgaaac ccaacgttgt
     1021 aaattgtggg gtcatacggg tcacggtcac accatcgggt tcgggcagaa aacacaatgt
     1081 aacttctagg tgaaatctga ttcacctgat cgatacaaag ttccatttgc acattactcg
     1141 gtagtggttt gctgctttcc agcttaaaac cgcccgtgct aatatcaatg atctgaccga
     1201 tcagttcacc agtatctttt ttcagcacgc gcatgtaata ggaaaaattt ctacgcggta
     1261 gttttcgttt ttcgttcgcc atctcgtcct ccttgcaaat ttgaaaggcg tcttaatttt
     1321 agtgcaaatg ccaaggaggt gcctgaaaac cagatgaaaa attatgaaat tgtaataatc
     1381 actcgcgccg taaaatatga gtcagaatcg tcgcaccaga accgccgata ttttgagcca
     1441 tacccacagt cgccccgtcc acctgggttg gtccacattc gccgcgcaat tgttgagcaa
     1501 cttccacgat ctgatacata cccgttgccc ctacaggatg accacgcgcc ttcaacccgc
     1561 ctcgcgtgca gactgggatc tttcctttga ttccaatctg attctccagc ccgagccgaa
     1621 ctccctgacc gcgctccgcg aaacccgtcg cttcgagaga caatgccgcc ataatggaaa
     1681 aagcgtcatg aagctcaaag acgtcaatat cttcaggcgt aatgccagcc attttgtacg
     1741 cctgttgtga agaaagataa gaagcgttca aaaacattgg gtctttgcgc gaatggacgg
     1801 ctattgtatc cgtggcagca ccggaagcgg ctacaaccac tttgggctgt cgcatcacct
     1861 tatcagcagg gacgataatc accgccgctg ccccatcccc caccggggaa gcatccaaca
     1921 aattgatagg tgttgccacc atagatgatt tctcaaattt ttcaacactt actttttcat
     1981 gcaaacgtgc atacgggtta tgcatggcat tggcatgtgc attgatggag aacggtgcaa
     2041 aatcttcgtg cttccagccg aactcgtgca tgtaacgtcg cattacaagg gcattgatac
     2101 caacaaatga aatcccttgt tccacttcat aatccgcatc ggctgcggtt gccaacgccg
     2161 cggtaacatc tttaccggct ttgtcggtca ttttttccac accaaccacc aaagcggact
     2221 caacatcacc cgaagcgacc gccatcagcg ccgaacggaa tgccgccgaa ccggagccac
     2281 aagccgcttc gatcttaacc gcttcctgtt tccacaaccc gatccaatcg ctaaaaaagg
     2341 caccgagctg attctgtccg cttaccatgg gagaaagcat attgcccaca tacaaggcat
     2401 cgactttgtc catgccggca tcttgcatcg caagaaatgc ggcttctccg ccgatctcgc
     2461 gcagggactt atcccaatgt tcatctactt ttgtctgacc gatcccaaga atggctactt
     2521 ttctcataac acctcagccc tgattctatc gtattcgcct ctcaggaact atctgaattt
     2581 ttgagcgtct ggttatttgg taggatagcc ccatatcccg tttatgctat cctagataat
     2641 acctaaggag aaacctcatg cagaaatatg gctttatatt gatcctactt attgtgctgg
     2701 tcataggctg taaaggagaa gaaaaatcat acgcctcccc aaccctttct gaagaatgga
     2761 caatcaagat gactcaatca ggcggaatca tgggattacg ccgttccatt caggtcgcct
     2821 cagatggaag ttacttgatc atagatgaac gtgcgcaaac ttcggtgaca ggtactctga
     2881 ctgaacaaca attaagtgaa ttaacagact taattgcgaa tctaaaattt atttcgcccg
     2941 ataatcgcgc gatctgtgct gattgctttg tctatgacct tgagatcgaa agcggcggaa
     3001 agaagatgat cctccaagta gaagatatca acctgcccga ttcagggatc gaagcattgg
     3061 tcaccttctt gcgcggaatt ttagagtctg cttgatacgg ctatgaatcg tttggaatgg
     3121 caggaaaaat attttcagaa agtagcagag tcttctgaag agggtgcaga ggcggtacgc
     3181 tatgtgcgcg agaaaaagat ccgggttgg
//
LOCUS       DAOQJE010000360         3208 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_19747_length_3208_cov_5.882017, whole genome shotgun sequence.
ACCESSION   DAOQJE010000360 DAOQJE010000000
VERSION     DAOQJE010000360.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3208)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3208)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3208
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19747_length_3208_cov_5.882017"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(228..677)
                     /locus_tag="PLT08_10090"
     CDS             complement(228..677)
                     /locus_tag="PLT08_10090"
                     /inference="COORDINATES: protein motif:HMM:NF024212.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HNE04857.1"
                     /translation="MSDSVQFSQSLRQWVDIFMHHSMRGWHHFVKSTGLTMPQFSILM
                     QLHYKGRIGMSEVSEQFGVSAAAASQLVDKLVQAGFMERAEDPSDRRAKLIQLSPNGK
                     KLIETGISERYRWMDGLTSKLSADDKAKVVDALQVLTEAARQLDETA"
     gene            complement(815..2431)
                     /locus_tag="PLT08_10095"
     CDS             complement(815..2431)
                     /locus_tag="PLT08_10095"
                     /inference="COORDINATES: protein motif:HMM:NF024583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing phosphohydrolase"
                     /protein_id="HNE04858.1"
                     /translation="MIQHISPEKLSARLAQIASEWYQLVYVCVDSNLSIIEKSPNISI
                     MFDGSSPIETGIPLTEAFKEFVGAEGALKAILSKKSPSFKIERINRYRSDGSLHYLTF
                     HVLPFDPGQPGLGLLVVFEDVTRFALSEQNLLQKRNELSLLQGALSYANAELDKRVEQ
                     RTAELEAAKKKIEQQLFRLQALRDNDIAILGTTDLRVGLRTIAEQAKRQLDVDLISIL
                     LFNAPLLTLETALVIGAQTHDLAKAQIHLGDTLIGRAALERRTLVFPDPANAFPQIDK
                     WAQIEKLHTCYAVPLIAKGNITGLLFLGNRSEIHPDEEWHDFLETLAGQAAMAIESMK
                     SFEDLQRSNFELALAYNRTIEGWSHALDLRDRETKEHTTRVTEMTMTLAKRAGMTEDE
                     ILHVKHGALLHDIGKMGIPDAILLKDGPLDNKEWAMMHKHPMFAYEMLFPIEYLRPAL
                     DIPYCHHEKWDGTGYPRGLKGDEIPLAARLFAVVDVWDALRSDRIYRKGWPLDKVLEY
                     IRSQSGTHFDPQVVDLFLLVMSEKKHNLSE"
     gene            complement(2435..2947)
                     /locus_tag="PLT08_10100"
     CDS             complement(2435..2947)
                     /locus_tag="PLT08_10100"
                     /inference="COORDINATES: protein motif:HMM:NF012255.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rab family GTPase"
                     /protein_id="HNE04859.1"
                     /translation="MTTIQKKICLLGDFGVGKTSLVQRFVEGRFDDKYLSTMGVKISR
                     KTLKRSYGKLNLLIWDVAGSNGFESSGTSYMQGAAGALIVCDLTRRDTLIAFENYARQ
                     IRKINPGIQLVFVCNKADLIHKRDIADMDLLNVLSGLGERNFFLTSAKTGEQVEDAFS
                     HLADTLEEGM"
     gene            complement(2960..>3208)
                     /locus_tag="PLT08_10105"
     CDS             complement(2960..>3208)
                     /locus_tag="PLT08_10105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE04860.1"
                     /translation="TPTPYEAFASGNVWIRQLPDFIDSRVEVLFISRPVKVLSVYDTW
                     VEVEWTDETGYHRGWVPARWITVIATVPAGQITLTANP"
BASE COUNT          835 a          733 c          738 g          902 t
ORIGIN      
        1 ccgccttgtt gaataccggt gttgacgatg cgcgaaagat aatcgggcag ggcaaggtcg
       61 aggtttgctt gcgcaaagat caatgcgatc gcaaacaata acatccaccg ataaggtttt
      121 acgaatttca ttaacttcat catacggaat aaaactcctt aatatcgaaa tatcacgata
      181 tgtttaacaa aagaatgtgt agaaaatttg tagaaggaag agagacttta tgcagtttcg
      241 tcgagttgac gtgccgcctc agtcaggact tggagcgcat ccaccacttt ggctttgtca
      301 tccgctgaaa gtttagatgt gagaccatcc atccaacgat agcgttctga gatgccggtt
      361 tcgatgagct tcttgccgtt gggggagagc tgaatcaact tggcgcggcg gtcactgggg
      421 tcttcggcgc gttccataaa gcctgcctga acgagcttat ccaccagttg gctggcagcc
      481 gctgcagaaa caccgaattg ctcgctcacc tcagacatgc caatgcgtcc tttgtagtga
      541 agctgcatca gaatactgaa ttgaggcatg gtcaacccgg tggatttgac gaagtggtgc
      601 catccgcgca tggagtggtg catgaaaata tccacccatt ggcgcagaga ttgtgagaat
      661 tggactgagt cactcatagt taagccttct ttttagttaa gcgggcttaa ctttatgcat
      721 tagttaagtt ttggtcaagg gatgagacgt tagaaaatta ttagaattca tacttccccg
      781 gcttatcgat ttaattgaat agtttttgat gaacttactc tgacaggtta tgcttttttt
      841 cagacatcac gagtaagaac aaatccacta cctgtgggtc gaaatgagtt cctgattgag
      901 agcggatgta ttcaaggact ttatccagcg gccaaccttt acgatagatg cgatcagagc
      961 gcagagcatc ccaaacatcg acaacggcaa agagacgtgc cgccagtgga atctcgtcgc
     1021 cttttagtcc gcgtgggtag ccggtaccat cccatttttc gtgatggcaa tagggaatgt
     1081 ctagtgctgg gcgcagatat tcaatgggaa atagcatttc atacgcaaac ataggatgtt
     1141 tgtgcatcat tgcccattct ttgttgtcaa gaggtccgtc tttaagcagg atcgcatctg
     1201 gaatacccat tttgccaata tcatgcaaga gtgctccatg cttaacatgc aaaatctcat
     1261 cctctgtcat acctgcccgt tttgcaaggg tcatggtcat ttcagttact cgcgttgtat
     1321 gttcttttgt ctctcgatct cgcaagtcta gggcatgaga ccaaccctca atagttcgat
     1381 tgtatgcaag tgcgagctca aagtttgaac gttgcaggtc ttcaaacgat ttcatgcttt
     1441 caattgccat ggcagcctgc cccgcaagag tctccagaaa atcatgccat tcctcgtctg
     1501 ggtgaatctc tgagcggtta cctaagaata gtaagcccgt gatattgcct ttggcgatta
     1561 gtggtacggc ataacaggta tgaagttttt caatctgtgc ccacttatct atttgtggaa
     1621 aagcgtttgc cgggtcggga aataccaaag ttcggcgttc taatgcggca cgtccgatta
     1681 aggtatctcc gagatgaatc tgcgccttgg ctaggtcgtg agtctgagcg ccaatgacta
     1741 gcgcagtttc cagcgtcagt aaaggagcat taaagagcag gatcgagatc aagtctacgt
     1801 ctagttgtcg ctttgcctgc tctgcgattg ttcttaatcc tacccgtagg tctgttgtgc
     1861 ccaggatggc aatatcatta tcacgaagcg cctgtaaacg aaaaagctgc tgttctattt
     1921 tcttctttgc agcctcaagt tcggcagtgc gctgctcaac gcgtttatcc aattcggcgt
     1981 tggcatagga aagagctccc tgtaagagac tcaattcgtt tcgcttctga agcaaattct
     2041 gttccgacag agcaaagcga gtcacatctt caaataccac caaaagcccc aaaccaggtt
     2101 gcccagggtc aaaaggtaaa acatgaaatg ttaaatagtg caaggagcca tctgaccggt
     2161 aacggtttat ccgttcgatt ttgaatgaag gcgatttttt agaaagaatg gctttgagtg
     2221 caccctctgc tccaacaaat tctttgaatg cttcagttag cggaatacct gtttcgattg
     2281 ggctagatcc gtcgaacatg atcgatatgt tcggtgactt ctcgataata gagagatttg
     2341 aatcaacaca tacatatact aattgatacc attcgctagc gatctgtgcc aagcgtgccg
     2401 ataatttttc aggagatata tgttgaatca ttggttacat accttcttca agagtatctg
     2461 ccagatgact aaacgcgtct tccacctgtt cgcctgtttt tgcgcttgtt aggaaaaagt
     2521 tcctttcacc caacccagat agaacattaa gcaaatccat atcagcgata tctcgtttat
     2581 ggatcaggtc tgctttgttg cacacaaaca cgagttgaat gcccgggttt atttttcgga
     2641 tctggcgcgc gtaattttca aaagcgatca gtgtatcgcg gcgggttagg tcacaaacta
     2701 ttaaagcacc tgcagctccc tgcatatagg aagtccctga agactcgaac ccattactgc
     2761 cagccacatc ccagatcagt aaatttaact ttccataaga ccgtttgagg gtcttgcgcg
     2821 atattttcac tcccattgtg cttagatact tgtcgtcaaa gcgcccttcg acaaagcgct
     2881 gtacaaggct ggtcttccct acgccgaaat cgcccagtaa acatatcttt ttttgaatag
     2941 tagtcatctt attgtccgat catggattgg cagtcaaagt aatttgcccg gctggtacag
     3001 tggctattac tgtgatccag cgtgcaggta cccagccgcg atggtagcct gtttcatcgg
     3061 tccattccac ttctacccag gtatcataaa ctgacaatac ttttacaggc ctgctgataa
     3121 acaacacttc cacacgcgag tcaataaaat ctgggagttg ccgaatccat acatttcctg
     3181 aagcgaaggc ttcataaggg gttggtgt
//
LOCUS       DAOQJE010000361         3207 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_19758_length_3207_cov_5.837563, whole genome shotgun sequence.
ACCESSION   DAOQJE010000361 DAOQJE010000000
VERSION     DAOQJE010000361.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3207)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3207)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3207
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19758_length_3207_cov_5.837563"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..274)
                     /locus_tag="PLT08_10110"
     CDS             complement(<1..274)
                     /locus_tag="PLT08_10110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE04861.1"
                     /translation="MDGLICGLIIAGIVVISFFFYLLNTARKKGNTDKNQFGKTEYAP
                     VYVSIADKPDSIIRGMNKFVAEARKTETAGDKWRWIPMLIFFAGIGL"
     gene            358..1521
                     /locus_tag="PLT08_10115"
     CDS             358..1521
                     /locus_tag="PLT08_10115"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HNE04862.1"
                     /translation="MKNVLYTAFDIVPSPKGASTHILHNLRGLVNGNFNVHLITPNDG
                     VLPPEDTIEGARLTRISQDLSQNFLARAVHFGKSVLSHLALNPGYDVVHYRNIWDGFH
                     ITQNKKKFGYKTIFEVNGLSSIELKYHYPGMDASLLAKIKEQELATLHLSDAIICPSN
                     VTRDYIASLGLNRKLVTVIPNGVSPSDFPVTPLPIRDGREPVLLYIGTLADWQGLDIV
                     IKALPKILEQQPVKLRILGRGRSRQRKMLAKQIRKLGLDEYVLVQPAVPHHEIPAVIA
                     SADLCVAPLGLNDRNVTQGACPIKVLEYMAAGRPLLASNMPIVRELVREDVDGLLFSP
                     NDPDDLARQANQLLKDMELSKRLAESAAGYVREKFTWHESQKKLLKVYEKLIK"
     gene            1589..2593
                     /locus_tag="PLT08_10120"
     CDS             1589..2593
                     /locus_tag="PLT08_10120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE04863.1"
                     /translation="MLDFIKEAGFPAIALLLGGVMVVIAIIRNIALEKVKIQLTSSQS
                     IQLAVVGVLFIIGGFVLQYFPPSSANTPENVDVYSTLYALQTQMVQPVNPQGQTNQTS
                     TPAGFALVSPTVTLFPNPVATLTPTLIPFTLTPDPSQPLAEVFCTNFYSIMVREGPND
                     NYPIKSVIENPNNLSNPDCMLFDFRMPDNSWIRIAANQDNPNYTQHELGWVTSDQFRP
                     IDFDKLRVYIPENVQDGLYCVTSRYGLKVRSCPNEGCREVGNLSLQDCLFMDGRSSDG
                     LWVRVSADKNDEKYASYKGNWVSTYFLAPFEFSAGYQPYFRYYFELLPTVEQIPTPNG
                     "
     gene            complement(2633..3037)
                     /locus_tag="PLT08_10125"
     CDS             complement(2633..3037)
                     /locus_tag="PLT08_10125"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF024675.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HNE04864.1"
                     /translation="MPISSIYSKTITIPSSTIDENGHVNNVVYVQWMQDIAIEHYASI
                     GGVEAQGPDATWVIRKHSIEYFLPAFEGEEIEVRTWVEDLRKVRSLRMYEFVRKSDGK
                     ILVKGESDWVFMDMKTGRPKAIPAEVENIFKP"
BASE COUNT          837 a          763 c          684 g          923 t
ORIGIN      
        1 ccagcccaat gcccgcaaag aagatcagca tcggaatcca acgccactta tccccagcgg
       61 tttcagtctt gcgcgcctca gccacaaact tgttcattcc acgaatgatc gagtctggct
      121 tgtcggcgat tgaaacatac actggcgcat actctgtttt gccgaattga tttttgtcgg
      181 tatttccttt tttacgtgcg gtattcagca agtaaaagaa aaaggagatt acaacgatcc
      241 ccgcgatgat aagtccacag atcagaccgt ccatccatgc ctccgagtta ttttatgcca
      301 gcggtcacga aaagcggact gcgcaatttg tgaccgcgct gggtataatt tctctcaatg
      361 aaaaatgttc tctacactgc cttcgatatc gtgccaagcc cgaagggtgc cagcacccat
      421 attctccaca atttgcgagg gttggtcaat gggaacttta atgtccatct tatcacgccc
      481 aacgatggcg tgctcccccc cgaagatact attgaaggcg ccagactcac gcgcatttcg
      541 caagaccttt cacagaactt ccttgcccgt gccgtgcatt ttggtaagtc agttttatcg
      601 cacctagccc tcaaccctgg ctatgatgtt gtccactatc gcaatatttg ggatggcttt
      661 cacatcacac agaataaaaa gaagtttggg tataaaacta tttttgaggt gaatgggctt
      721 tcttccattg aactcaaata tcattacccc ggtatggatg ccagcctgct cgccaagatc
      781 aaagaacagg aattggcgac tctgcacctg agcgacgcca tcatctgccc atcaaacgtg
      841 acgcgcgatt acatcgcctc gctcggtctt aaccgcaagc tggtgactgt tatcccgaac
      901 ggagtcagcc cttcagactt tcccgttacc cctttgccca ttcgagatgg acgcgagccg
      961 gttttgcttt acatcggtac attagccgat tggcaaggct tggatatcgt catcaaagcc
     1021 ctgccgaaaa tccttgagca acagcctgtc aaactccgca tcctcgggcg cggacgttcc
     1081 cgtcagcgta agatgttggc aaagcaaatt cgcaaactgg gtttggatga atatgttttg
     1141 gtgcaacctg ctgtaccgca tcatgagatt ccagcagtga tcgccagcgc agatctttgt
     1201 gttgctccac tcgggttgaa tgaccgcaat gtgactcaag gtgcttgccc gatcaaagtg
     1261 cttgagtata tggcggctgg gcgaccttta ctcgcctcaa atatgcccat tgtccgcgaa
     1321 ctggtgcgcg aagatgtaga cggattgctc ttttcaccca acgacccaga cgacctagcg
     1381 cgacaggcaa atcaattact gaaagatatg gaactatcca aacgcttggc tgagtctgcg
     1441 gcgggatacg tccgcgagaa atttacatgg cacgagtcac agaagaaatt gttgaaagtg
     1501 tacgaaaaac taataaagta acagaccttc ttttttgttg aaaatctacc ccggtttcag
     1561 tatataattt tcgaatagga ggagcaacat gttagatttc attaaagaag ccggttttcc
     1621 cgccattgct ttgttgctcg gaggggtaat ggttgtaatt gctatcatca gaaacattgc
     1681 ccttgaaaaa gtaaaaatac aacttacttc aagccagtct attcagttgg cagtggtggg
     1741 tgtattgttc attataggtg ggttcgtttt acagtatttt cctccttctt ctgcaaatac
     1801 ccctgaaaat gttgatgttt attccacgct ttatgcctta cagacccaaa tggtgcagcc
     1861 tgtaaaccca caaggacaaa ctaatcagac ttctacacct gctggtttcg ctttggtttc
     1921 gccaactgtt accttgtttc caaacccagt ggcgacccta acaccaacct taatcccctt
     1981 cacactaact ccagatcctt cacaaccatt agcagaagtg ttttgcacga atttttatag
     2041 cattatggta cgtgaaggac ctaatgataa ttacccgatc aagtctgtta ttgagaaccc
     2101 caataatttg agtaacccag attgtatgct tttcgatttt cgcatgccag ataactcatg
     2161 gatacgtatt gctgcaaatc aggataaccc caattacacc caacatgaac ttggttgggt
     2221 gacttcagat caattccgcc cgattgattt tgataaattg cgagtatata tccctgaaaa
     2281 tgtgcaagat ggtttgtatt gtgtcaccag ccgatatggt ttgaaagtgc ggagttgccc
     2341 caatgagggt tgtcgtgaag ttggcaacct gtctttgcaa gactgtcttt tcatggatgg
     2401 gcgttcttca gatggtttat gggtgagagt ttcagccgat aaaaatgatg agaaatatgc
     2461 ttcttataaa ggaaattggg tgagcactta tttcttagcc ccctttgaat tttcggctgg
     2521 gtatcaacca tatttccggt attattttga attattacct actgttgaac aaatcccaac
     2581 cccgaatgga taacgaagaa ccaggtcacg cttcaaacgt gacctggttc ttttacggct
     2641 tgaatatatt ttctacttcg gcgggaattg cttttgggcg tcctgttttc atatccataa
     2701 aaacccagtc actttcgccc tttactaaaa tcttcccatc actcttgcgg acaaattcat
     2761 acatgcgcag cgaccgtact ttgcgtaaat cctccaccca agtgcggacc tcaatctctt
     2821 cgccttcgaa ggctggcaga aaatattcga tgctatgttt acggatcacc caggtggcgt
     2881 caggtccctg ggcttctaca cctccgatgg atgcataatg ttcaatggca atatcctgca
     2941 tccattgcac atagacaaca ttgttgacgt gtccattctc gtcaatagtt gaagagggga
     3001 ttgtgattgt tttggaataa atagatgaaa tcggcatttt ttgattttac agtgacttcc
     3061 cccaaacggg ggagtttatt tttattgccc cactgataga atgaatttaa taaaaatcat
     3121 ttttttggag aaattaaatg ggtccaatca tcggtggcac ttcattaata attactgtaa
     3181 tttcaatcgc ttgtggatgt ttgatta
//
LOCUS       DAOQJE010000362         3194 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_19874_length_3194_cov_5.794521, whole genome shotgun sequence.
ACCESSION   DAOQJE010000362 DAOQJE010000000
VERSION     DAOQJE010000362.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3194)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3194)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3194
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19874_length_3194_cov_5.794521"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            171..2759
                     /gene="recG"
                     /locus_tag="PLT08_10130"
     CDS             171..2759
                     /gene="recG"
                     /locus_tag="PLT08_10130"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:NF008168.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HNE04865.1"
                     /translation="MPAMIKLTIMQPSLEKLRKFFRLEHENKYENTAIIGGLAKMLDY
                     WEGEARADGVAEEVIQAVVSRLRAYEKLNPTGRADSLKGLWKRISETYPEAGQKPKAP
                     PPPQRPNPQPQRPQRSDEEAKPKADVAEQPAVQNQSQPKQHPQQHQQKQQRPNNSPQR
                     FESATSAKHSQTPAALGAKLTVLNGVGPKNAESLAKLGMQTLGDMLYSFPRRYEDYSL
                     LKPIQDLMYNDVVTVLGTIQSVHNRPIRGGKQSIIEVIISDGTGSLRVSFFNQPWLAN
                     RFKTGDAISVSGKIDQYLGRIVMNSPDWENVEMESLSTNRIVPIYSLTERITQKWLRN
                     QMKQVVTFWAPAVVDALPESIKHDAQLVSLGEALTQVHFPDTQDRLKKARERLAFDEI
                     FYLQMGVLRQKRDWQVVEGKRFTASDEWLGARLAALPFTLTSAQQEALNDIRGDLDSG
                     KPMNRLIQGDVGSGKTVIAAMGASIVVSTGAQAAIMAPTSILAEQHYRNFSNLLTGEN
                     GFLQKEEVRLLVGNTSEAEKEAIRQGLADGSIKIVIGTHAVIEPDVTFKELQFIVIDE
                     QHRFGVEQRAELRSKGTNPHLLVMTATPIPRSLALTVFGDLDISVMNVMPVGRQPIAT
                     YVLRPQERERAYTMIRSQVKNGNQAFIVYPLIEESEKINVRAAVDDFETLSTQVFPDL
                     KLGLLHGRMKPSEKDEVMLKFRDKEFDILVSTTVIEVGVDVPNSTLMLIEGADRFGLA
                     QLHQLRGRVGRGSVASTCLLIPTHEDATENERLQAMASTNDGFELADLDLKLRGPGEF
                     LGTRQAGFASSLKMASITDVVLIEKAREQAKSLFERDPDLSQPEHALLAEAFERFWMG
                     TSSDVS"
     gene            2863..>3194
                     /gene="coaD"
                     /locus_tag="PLT08_10135"
     CDS             2863..>3194
                     /gene="coaD"
                     /locus_tag="PLT08_10135"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004595 - pantetheine-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HNE04866.1"
                     /translation="MVRALFPGTFDPIHYGHMDIANRASKLFDEVVMAVYDKPLKNLL
                     FSPEERMSLVNEAFKDNPKIKVTGYSGLTIEFAKKIDAQVIVRGLRVFSDFEFEFRMA
                     LANQRLAGA"
BASE COUNT          838 a          720 c          832 g          804 t
ORIGIN      
        1 gccagttcaa aaagcctgct gttgttgaac caattcatga ttttctccta atttagtatt
       61 aataatttga taatagtgtc aatttgttat taagtcaagc cctttcagta ggttttttgg
      121 gggataagta tcaaattcaa agctattcga gggatattga gaatcggttc atgcctgcca
      181 tgataaaatt gactattatg caaccgtctt tggaaaaact acgcaaattt tttcgactcg
      241 aacacgaaaa caaatacgaa aataccgcca tcatcggcgg acttgccaaa atgttggatt
      301 actgggaagg ggaagcccgc gccgatggcg tggcggaaga agttattcaa gctgttgttt
      361 cccgtttacg tgcttatgaa aagttgaatc ccaccgggcg ggcggattcc cttaagggct
      421 tgtggaagcg catcagtgaa acctacccgg aagcagggca aaagcccaaa gcgccaccac
      481 cgccgcagag accgaatcca cagcctcaac gtccgcagcg aagcgatgag gaggcgaagc
      541 caaaagcgga tgtcgctgaa caacctgcgg tgcagaatca gagtcagccg aagcagcatc
      601 ctcaacaaca ccaacaaaaa cagcagagac caaataattc tccgcaacgt tttgaatctg
      661 cgaccagtgc caagcatagc caaacacctg ctgcgttggg cgccaaactt acggtcttga
      721 atggggtggg accgaagaat gctgagtcgt tggcaaagct tggaatgcaa acgctggggg
      781 atatgttgta ttccttcccg cgtcggtatg aagattattc actgctgaaa ccgattcaag
      841 atttgatgta caacgatgtg gtgactgtgt taggcaccat tcaaagtgta cataaccgtc
      901 cgattcgcgg tggaaagcaa agcattatcg aagtcatcat ttctgatggc acaggctcat
      961 tgcgagtctc gttcttcaac caaccttggt tggcgaatcg tttcaagacg ggcgatgcga
     1021 tttctgtttc tggaaagatc gatcagtatc ttggacgcat cgtaatgaac agccccgatt
     1081 gggaaaatgt tgaaatggaa agtctcagta ccaatcgtat tgtgccgatc tattcgctca
     1141 ctgaacgcat cactcaaaaa tggttgcgta atcagatgaa acaagttgtt acgttttggg
     1201 cgcctgctgt ggtggatgcg ttaccagaaa gcatcaaaca tgatgcgcaa ttggtttcac
     1261 tgggtgaagc acttacacaa gttcattttc ctgatacgca agaccggctt aagaaagcgc
     1321 gggaacgcct tgcctttgac gaaatttttt atttgcaaat gggtgtgttg cgacaaaagc
     1381 gtgactggca agtggtggaa ggcaagcgct tcaccgcctc agacgagtgg ctgggtgcgc
     1441 gtttagcggc tttacctttc actctcacct ctgcccagca agaagcactc aatgatattc
     1501 gcggtgattt agactcgggt aaaccgatga atcgactcat tcaaggggat gtgggttccg
     1561 ggaagaccgt cattgcagca atgggtgcga gcatcgtggt ttctacagga gcccaagctg
     1621 ctattatggc gccgacttct attttggcag aacagcatta tcgtaacttc tcgaacttgc
     1681 taacaggcga aaatggcttc ttacaaaaag aagaggttcg cctcttggtg gggaatactt
     1741 ctgaagcgga gaaagaagcg atccgccagg ggcttgccga tggctcgatc aagattgtga
     1801 ttggcaccca tgcggtcatt gaaccggatg tgaccttcaa agaattgcag tttattgtga
     1861 tcgatgaaca acatcgtttt ggtgtagaac agcgcgctga gttgcgcagc aaaggtacca
     1921 atccgcacct tttggtgatg acggctactc caattccgcg ctcactggcg ttgacggtct
     1981 ttggtgacct tgatatttca gtgatgaatg tgatgccggt gggtaggcag cctattgcga
     2041 cctatgttct ccgcccgcag gaacgcgaac gtgcatatac gatgattcgc tcgcaggtga
     2101 aaaatggcaa tcaggcgttc attgtgtacc cactcattga agagagcgag aagatcaacg
     2161 tccgcgcggc ggtggatgat ttcgaaacat tgtctacaca ggtgtttcct gacctcaagc
     2221 ttggtctctt gcacggacgg atgaagccca gcgaaaaaga tgaagtgatg ctcaagttcc
     2281 gcgataagga atttgatatt ctcgtttcaa cgacggtgat cgaagtaggc gtggatgtgc
     2341 ccaactcgac cttgatgttg atcgaaggcg cagatcgttt cggtttggct cagcttcatc
     2401 agctacgcgg acgtgtcggt cgcggttcgg tggcgtctac ttgtttgctt attcccacgc
     2461 acgaagatgc gactgaaaac gaacgtctac aggcgatggc gagtaccaat gatggttttg
     2521 aattggctga cttagacctc aaattacgtg gtcctggtga attccttggg acgcgccagg
     2581 cagggtttgc atcttccttg aagatggcga gtattaccga tgttgtgctg attgaaaaag
     2641 caagagagca agccaagtct cttttcgaac gtgacccaga cctttctcaa cccgagcatg
     2701 ctttgttggc tgaagctttt gaacggttct ggatgggaac gtcgagcgac gtgagttagt
     2761 tgaatagttt cgtagttgaa gggcaaatgc acactatgag acgacgcaac caatcgacca
     2821 cccgactaca agactaaatc actaaaggat cacaagacta caatggttag agccttattt
     2881 cccggaacgt tcgaccccat ccactacgga catatggata ttgccaatcg cgcttctaag
     2941 ctcttcgatg aagtggtgat ggcggtctat gataagccgc tcaagaattt gttgttttct
     3001 cccgaagaac gcatgtcttt ggtcaacgag gcgttcaaag ataacccaaa gatcaaagtc
     3061 accgggtata gcgggctgac cattgaattt gcgaaaaaga tcgacgcaca agtcatcgtc
     3121 cgcgggcttc gtgtattctc tgatttcgaa tttgagttcc ggatggcgtt ggcaaatcag
     3181 cgccttgctg gtgc
//
LOCUS       DAOQJE010000363         3193 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_19884_length_3193_cov_7.272148, whole genome shotgun sequence.
ACCESSION   DAOQJE010000363 DAOQJE010000000
VERSION     DAOQJE010000363.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3193)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3193)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3193
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19884_length_3193_cov_7.272148"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..464)
                     /locus_tag="PLT08_10140"
     CDS             complement(<1..464)
                     /locus_tag="PLT08_10140"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HNE04867.1"
                     /translation="MSKKKTSSELEKELLDAKQRIAELEQNEARFATILENSPVAIGI
                     SRLSDLKLIYVNSAFADLFGYSQDEILKNSTVDLGIWKDLEDRQKFFEIVRNKNKVTG
                     FEMTVRPQQGSEKNILVKGEMVEIDKQPCFVSQVIDITDLKQSEAALQASEA"
     gene            697..2223
                     /locus_tag="PLT08_10145"
     CDS             697..2223
                     /locus_tag="PLT08_10145"
                     /inference="COORDINATES: protein motif:HMM:NF013630.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HNE04868.1"
                     /translation="MKILLATKLTAKFLLPIFASAILILTQAGKGILNLTDAPAPELH
                     PEIKDSSSSFDTLRSATQPAQPTQASIVADTISYQIEAPVLPAYPFANPAGCSAAQPN
                     LTPEQMLTNVQNAIGNEYNLAERRQLYVWNGEQGSMLNVTNDPFIYEFTFNEFQHPLT
                     YRGDKVATIFMTQGFVVWFREYSDSFRLTAIPMTNGVMQSAWAPYVTSYWQQNGQPND
                     EKIYPVMKKLPCHWVIDSGYVTNETLTTMFNLDWHTPDYLSAGRQYLASTCKEAYQIS
                     QEKVGYWDATSMCGPLAWTIMRDVNAFPYRIGSWSQNAATFTRVNPRWDGQPWGSFDP
                     ETFTLFHTEEAIAGYDFQSKGNLYTGDVVYTFATLYVTAGYFDHIFVVAGVDENNTRL
                     AISNMVQNSPYADCSIEEVKLYTPGDRETGVLNHEWNGNGYGKTGTTGFDVFRWNWIT
                     YHNNGQAMSYTVRWGETIETIAFDWKISPESLQAANQFSADTQLTPGQIIILPAPTQS
                     "
     gene            complement(2296..2940)
                     /locus_tag="PLT08_10150"
     CDS             complement(2296..2940)
                     /locus_tag="PLT08_10150"
                     /inference="COORDINATES: protein motif:HMM:NF015438.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MOSC domain-containing protein"
                     /protein_id="HNE04869.1"
                     /translation="MKLISINAGKKHTLVNKGKTEITGIYKNPLAGAVHITKLGIADD
                     YIGSPKHHGGPDQALYVYGEADYQWWEKEIGTKLGPGMFGENLTISELACSGFNIGDY
                     LYIGNVTLQVTAPRIPCGTFASKMEDPQWVKKFSAAERPGFYVRVLQEGELSASEEVR
                     VEKYTGATLSLVQVYRDHYDKEGRNEAVLRRHIDSPLSIRMREKYERELEALLH"
BASE COUNT          863 a          868 c          617 g          845 t
ORIGIN      
        1 tttgcctcac tggcttgcaa cgcagcttcc gactgcttca aatcagtaat atcaatgacc
       61 tgtgatacaa aacaaggttg tttatcgatc tcgaccatct cccctttaac aaggatattt
      121 ttttcactac cctgctgtgg gcgaacggtc atctcgaaac ccgtcacttt attcttgttg
      181 cgtactattt caaaaaactt ctgccgatct tcaagatcct tccatattcc caaatctacc
      241 gtagagtttt tcagtatttc atcttgtgaa tatccaaaca gatctgcaaa cgccgaattc
      301 acatagataa gtttcagatc actcagccgt gaaattccaa ttgcaactgg gctattctct
      361 aaaatagtag caaaacgcgc ttcattttgt tccagctctg caatgcgctg tttagcatct
      421 aataattctt tttccagttc agaactggtc ttctttttgc tcataattta cttcctataa
      481 accctgatag aaacactctt aatatatttt tgcatagtat cgaaaacgag agaatggggg
      541 aattccctac ctgtaagttg cgtaaatcaa ccatctcatt ggcaggaatt tacccacgca
      601 aaattaggca aaaattacta aagaaatact agagaacacg tctgaggaaa cccagcctta
      661 aatgaaataa tgccgccaac acaaaaggtt gatggcatga aaattcttct cgctacaaaa
      721 cttaccgcaa aattccttct ccccatattt gccagcgcaa tccttattct cacacaagct
      781 ggcaagggga ttctcaactt aaccgacgcc cccgcccctg aacttcaccc tgaaattaaa
      841 gactcttcct caagtttcga cacgcttcgt tccgcgaccc agcctgctca acctacccaa
      901 gcttccatcg tcgccgatac gatttcctac caaatcgaag ccccggtgct tcctgcttat
      961 cctttcgcca accctgccgg ttgctccgct gcccagccaa acctcactcc tgaacaaatg
     1021 cttaccaacg tacaaaatgc catcggcaat gagtacaacc tcgctgaacg ccgtcagctg
     1081 tatgtatgga acggggaaca aggcagcatg ctaaacgtca cgaatgaccc gtttatttat
     1141 gaattcacct tcaatgaatt tcagcatccg ctcacctatc gcggagacaa agtcgccacc
     1201 atcttcatga cccaaggctt cgtcgtttgg ttccgcgaat atagcgatag ttttcgcctt
     1261 actgcaatcc caatgaccaa cggcgtgatg caatctgctt gggcgcctta tgtcacttcg
     1321 tattggcagc aaaacgggca acccaacgat gaaaaaatct acccagtcat gaaaaaactt
     1381 ccatgccatt gggtgatcga ctctggttat gtgaccaatg aaactctcac caccatgttc
     1441 aacctggatt ggcacactcc cgattacctt tcggcaggtc gtcaatattt agcaagcacc
     1501 tgcaaagaag cgtaccaaat ctctcaggaa aaagttggct actgggacgc cacctcgatg
     1561 tgcggacctt tggcatggac gatcatgcgt gatgtaaacg ccttccctta tcgcatcgga
     1621 agttggtcac agaacgctgc gacattcacc cgcgtgaacc cccgctggga cggacaaccc
     1681 tggggatcgt tcgacccaga gaccttcact ctcttccata cagaagaagc cattgccggt
     1741 tatgattttc aatccaaagg aaatctctac acaggcgatg tggtttacac ctttgccacc
     1801 ctatatgtaa ccgctggata ctttgaccat atttttgtcg tcgctggtgt ggacgagaac
     1861 aatacccgct tggcaatctc gaacatggtg cagaactcac cgtatgcaga ttgctcgatt
     1921 gaagaagtaa aactctacac ccccggcgac cgcgagaccg gcgttctcaa ccatgaatgg
     1981 aatggaaacg gatacggcaa aactggaacc acaggctttg acgttttccg ctggaactgg
     2041 atcacctacc acaacaatgg acaagcaatg agctataccg ttcgctgggg cgaaacgatt
     2101 gaaaccattg cgtttgattg gaaaatctcg ccagaaagct tgcaagctgc caatcagttc
     2161 tcggcagata cacaacttac tcctggtcag attattattt tgcccgcgcc cactcaatca
     2221 taaaaattaa ttttgggccc ctcctgggca agcatgcaag aagagaacag ccaccatatg
     2281 gtggctgttc tcttcttaat gaagcaaagc ttctaattca cgttcatatt tttcccgcat
     2341 ccgaatggac agaggtgagt caatatgcct tcgcaggaca gcctcatttc gcccttcttt
     2401 atcataatgg tcgcggtata cctgcaccaa agaaagggtt gcccctgtat atttctccac
     2461 acggacttct tcactcgctg aaagctcccc ttcttgcaat acgcggacgt aaaacccggg
     2521 gcgctcggct gcgctgaatt tcttcaccca ttgcgggtct tccattttcg aagcgaacgt
     2581 cccacaggga atgcgaggag ccgtcacttg taacgtcacg ttgccaatat aaagataatc
     2641 tccaatatta aaaccgctac atgccaattc actaatagtg agattctctc caaacatgcc
     2701 gggacccaac tttgttccaa tctctttttc ccaccattga taatccgctt cgccataaac
     2761 ataaagcgct tgatcgggtc cgccgtgatg ttttgggctg ccaatataat catcggcaat
     2821 tccaagcttg gtaatgtgca cagctccagc gagcggattt ttgtagatgc ctgtaatctc
     2881 cgtcttacct ttattgacta gagtgtgttt ctttcctgca ttgatgctga ttagtttcat
     2941 tctatatcct ttaaatacaa acgtgctgac atgagcagat tttatcccat ccatgccagc
     3001 acgaacccta ctttggaatt attttagtaa tttgttgtgc ggtcgatcag ccctctttcg
     3061 cgggcaatcc cgtcacacca gcggaagaca tacacactga tcaccccaaa gaaaatgctc
     3121 aacccgacca tgacgccaag aatttgcaag ttactgaagt ttgccaaggt tgggaaggct
     3181 tgcgccacat tcc
//
LOCUS       DAOQJE010000364         3186 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_19957_length_3186_cov_4.571383, whole genome shotgun sequence.
ACCESSION   DAOQJE010000364 DAOQJE010000000
VERSION     DAOQJE010000364.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3186)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3186)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3186
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19957_length_3186_cov_4.571383"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1412
                     /locus_tag="PLT08_10155"
     CDS             <1..1412
                     /locus_tag="PLT08_10155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004819 - glutamine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006425 - glutaminyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glutamine--tRNA ligase/YqeY domain fusion
                     protein"
                     /protein_id="HNE04870.1"
                     /translation="LGIEWVKVTYASDYFDELYDWAKKLVLMGKAYVDDQTPEEVSKN
                     RGTLTQPGVNSPYRDRDVEENLDLLERMKNGEFPDGTRILRAKIDMAHPNINMRDPAM
                     YRIIHTPPHHRTGDKWKIYPMYDWSHGQNDSMEGITHSLCSLEYENHRPLYEWFLEQL
                     GVFKPRQIEFARLNMNYTVMSKRKLRRLVEEKLVSGWDDPRMPTLRGMRRRGYTAEAV
                     LDFIRRVGVAKNYSVSDVALLEECVRDDLNKHALRRMAVIRPLKVVIDNYPDDLVEEM
                     SAINNPEDAEAGTRKVPFSKVLYIEQDDFREVPPAKYYRLFPGNEVRLRYAYFIKCTH
                     VVKNDAGEVIEVHATYDPSTKGGDSPDGRKVKSTIHWVSAKHAKEVEVRMYDRLFNKE
                     NPEEGEEGFLNCLNPNSLEILHGYVEPALVSAETGSRFQFERLGYFCVDKEATSEKPI
                     FNLTVNLKDTWAKFEKKAK"
     gene            1422..1922
                     /locus_tag="PLT08_10160"
     CDS             1422..1922
                     /locus_tag="PLT08_10160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE04871.1"
                     /translation="MKLDFRNRMLVSLVAIAVTACAPQAPATPTVDIVGTVAAQLASE
                     MLTQTAGAVTPTPIPPTETPTPAFTDTPAVPTEKPQIKPPAVVVFTGCWTGPGESYTN
                     ISNIDPSIKKNGRQNVVILGTGTEPGWIVIRNPYFNNPCWVKVESMSIDPNMDLSGYP
                     QMTPGP"
     gene            complement(1960..2682)
                     /locus_tag="PLT08_10165"
     CDS             complement(1960..2682)
                     /locus_tag="PLT08_10165"
                     /inference="COORDINATES: protein motif:HMM:NF019844.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0070064 - proline-rich region binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HNE04872.1"
                     /translation="MGLLFVFFVSACGLFGTSDIDTLSTVVAKTMTAVALASTDTQVI
                     PVSTVITEVSTPTLVSTDILYVKTTADNVNLRVNPGRLFQVSRVLTRGSRMEYLGILP
                     NGQWLKVRTGEGIVGWVLKELVEADFELVGPLAVPDNVVLITGTVLDTNNNLVNGIEI
                     SISKDEQGDRTLTSKEGTYYLYLPTTLSGVWSLKQTAISCESNLMGSDCKCLNSSCGA
                     FDPIEYRLQFPLVPGLYDFVVK"
     gene            2806..3021
                     /locus_tag="PLT08_10170"
     CDS             2806..3021
                     /locus_tag="PLT08_10170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE04873.1"
                     /translation="MSETKRKSMKDKLPPEALEHMKAARAEMRKSMETILPPGFLEHR
                     RAARKEALLAFRSLIDAAIKKTEEKSS"
BASE COUNT          949 a          775 c          690 g          772 t
ORIGIN      
        1 ggcttgggat tgaatgggta aaagtaacat acgcatcaga ttattttgat gaactttacg
       61 actgggcaaa gaaactggtt ttgatgggca aagcctatgt agatgaccag accccagagg
      121 aagtaagcaa gaatcgcggc acgctcacgc aacccggcgt aaactctccc tatcgcgacc
      181 gcgatgtaga agaaaactta gacttgctcg aacgcatgaa gaacggcgaa ttccctgacg
      241 ggactcgtat tttacgcgcc aagattgata tggcgcatcc gaatatcaac atgcgtgacc
      301 cggcaatgta tcgcatcatc cacacccccc cgcatcaccg taccggtgac aagtggaaga
      361 tttacccaat gtatgattgg tctcatgggc agaatgattc gatggaaggt atcactcatt
      421 cgttatgttc gcttgagtat gagaatcatc gtcccttata tgaatggttc ttagaacagc
      481 ttggcgtttt caaaccacgt cagatcgagt ttgcgcgttt gaacatgaac tatacagtga
      541 tgagcaagcg caaacttcgc cgccttgtcg aagaaaaact ggtcagcggt tgggatgacc
      601 cccgtatgcc aacattgcgc ggtatgcgca ggcgcggcta caccgctgaa gcggttttgg
      661 atttcattcg ccgtgtgggt gtggcaaaaa attatagcgt tagtgatgtg gcactactcg
      721 aagaatgtgt acgtgatgat ctcaacaaac atgctttgcg tcgcatggcg gttatccgcc
      781 cgctgaaagt agtcatcgat aattaccctg acgacctcgt cgaagagatg agcgccatca
      841 acaaccctga agacgctgaa gccggcaccc gcaaagtgcc tttctctaaa gtcctttaca
      901 ttgaacagga tgatttccgt gaagttccgc ccgcgaaata ttaccgtctc ttccccggta
      961 atgaagtccg cttgcgatat gcatatttca tcaaatgcac ccatgtggtg aaaaatgatg
     1021 ctggtgaagt gatcgaagtc catgccactt atgacccatc cacaaagggt ggcgattcac
     1081 ctgatgggcg aaaagtgaaa tctacgatcc attgggtttc tgctaaacat gcaaaagaag
     1141 tggaagtccg catgtatgat cgtctcttca acaaagaaaa tccagaagaa ggtgaagaag
     1201 gattcctaaa ctgtttgaat ccgaattctt tggaaattct ccatggatat gtagaaccgg
     1261 ctcttgtctc cgccgaaact ggttcacgct ttcaatttga gcgtctcgga tatttctgcg
     1321 tagataaaga agcaacttct gaaaaaccta tcttcaactt aaccgtcaac ctaaaagata
     1381 cttgggcaaa gtttgaaaag aaagcaaaat aggatcattg aatgaaactt gattttagaa
     1441 accgaatgct cgtgtctcta gtagcgattg cagttacggc atgtgcacca caagctcctg
     1501 ctactcctac tgtggacata gttgggacag tagccgcgca acttgcgtca gaaatgctga
     1561 cccaaacagc cggtgcggtt acccctaccc caatcccacc cacagagaca ccaacccctg
     1621 cgtttaccga cactccagcc gttccaactg aaaagcctca gattaagcca ccagcagtgg
     1681 tagtattcac tggatgttgg acgggacctg gtgagagtta tacaaacatt agcaatattg
     1741 atccaagcat aaaaaagaat ggcagacaaa atgttgttat cctcggtaca ggtacagagc
     1801 cagggtggat agtgattaga aatccctact ttaataaccc ttgctgggtt aaagttgaaa
     1861 gtatgtctat cgaccctaac atggatctgt ccggatatcc acagatgaca ccaggaccat
     1921 aaaaaaacag cggctgaatt cagccgctgt tttttttatc tacttcacta caaaatcata
     1981 caaccccggc acaagcggaa actgaagtcg atactcgata ggatcaaaag ctccacagct
     2041 tgaattcagg catttacaat ctgaacccat caaattactt tcacaactta ttgccgtttg
     2101 ctttaaagac caaaccccgc ttagagttgt gggcaaatac aaataataag ttccttcttt
     2161 tgacgttaga gtacggtcgc cttgttcatc tttggagatg ctaatctcaa tcccatttac
     2221 gagattattg tttgtatcca atacagttcc agtgatcaat accacattat ctgggaccgc
     2281 cagcggaccg acaagttcaa agtctgcttc taccaattct ttcaaaaccc aaccgacaat
     2341 gccctcccct gtgcgaacct tgagccattg tccatttggc aaaatgccca aatattccat
     2401 tcgtgaaccg cgagtcaaca cacggcttac ctgaaacaac ctccccgggt tgactcgaag
     2461 attgacatta tctgcagttg ttttcacata aagtatgtct gttgacacta gcgtaggagt
     2521 tgaaacttct gtgataacag tactgacagg aataacttgc gtatccgttg aagccaatgc
     2581 taccgccgtc attgttttag caacaacagt ggaaagagtg tcaatgtcag aagttccaaa
     2641 aagcccacag gctgaaacaa agaagacaaa aaggagtccc aaaagcagcc tcatagaaat
     2701 gtagttgttt tgtttcatat attcctctca ctcaatttta tacgatttac aaatacaaac
     2761 ggattctcat cggtgctata atcacaatga aacaaggagt gtcctatgag cgaaacaaaa
     2821 cgcaaatcca tgaaagacaa acttccgcct gaggcattgg agcatatgaa ggctgcccgt
     2881 gcagagatgc gcaaaagcat ggagaccatc ctcccgcccg gctttttgga gcaccgccgc
     2941 gctgctcgca aagaagcgct actcgccttc cgcagcctga tcgatgctgc catcaaaaag
     3001 acggaagaaa aatcgtcata aagcaaaacc cccagccgtt tggctgggag ttttttatct
     3061 atcgaaatgg acgtcgacct cgcaccatga acaaggtgta aaacaaagcg atccctgccc
     3121 cgccaaacag cccctgccca agcaagacat gagcatcata taaaatcgca cctgcgataa
     3181 gtaaac
//
LOCUS       DAOQJE010000365         3184 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_19981_length_3184_cov_3.782998, whole genome shotgun sequence.
ACCESSION   DAOQJE010000365 DAOQJE010000000
VERSION     DAOQJE010000365.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3184)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3184)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3184
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19981_length_3184_cov_3.782998"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            295..2469
                     /locus_tag="PLT08_10175"
     CDS             295..2469
                     /locus_tag="PLT08_10175"
                     /inference="COORDINATES: protein motif:HMM:NF024583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HNE04874.1"
                     /translation="MNSKQPRHTPTFRASQLAVLNKLISMTHAWTDTQTVLSMALEQA
                     AQLAMADGAECHLVNSSGELQFTTQYNLDIVFMSGSLDIRFPIGKGLPGLAISNQSAF
                     LIPDIENEENYQRQHLAQQARYCSLICVPLSGMNSLLGTIMLYFRKRIQPDAVLLEIL
                     TAIGQHLGVSIERAHLFQQTSEQLKELQILQTVANALNRSANIQEALEQSLEAVVTTM
                     NMRCGWVVLLDGFQKNRLAASYNLPPELDPADWSAMRTHCRCIELLQLGKLDAAIKIV
                     ECQQLKKVKSPDYPYHSHASIPVRVGTLLLGNLNIVPPSGSAITIDNYRLFNSIGDQI
                     GVAIERARLYEQAKEQRTLEQQILLGHGQMLLGERKLQIIVNQTIKVVSNALRVEYVT
                     LVLVASDGKISLETDLGFSPRKTRDTVDVLLADNSAIVQSIRLKMPIINPDINLERQL
                     KTNLVDEKIIMTSSLIVPMLMGEEALGSIAVYSQSPRQWSEDEIRLLSLLANQTAIAI
                     ENTRLLEAEHTARKHAEILHLQTIQQAQDLTLAYDTTIEGWSRALDLRDKETEEHTLR
                     VTNLTLQLAQAFDISHVELKHIKHGALLHDIGKMGIPDNILRKAGELSDDEWVMMRQH
                     PQLAYEMLSPIAYLLPALDIPYCHHEKWDGTGYPRGLKGEEIPLAARLFAVVDVFDAL
                     TSDRPYRPAWAKNKAIEYIRQKAGSHFDPSVVEVFLRLIDNK"
     gene            complement(2528..>3184)
                     /locus_tag="PLT08_10180"
     CDS             complement(2528..>3184)
                     /locus_tag="PLT08_10180"
                     /inference="COORDINATES: protein motif:HMM:NF012257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HNE04875.1"
                     /translation="LQDFEFLRGLDHETMTALANSAMWKVFPPHAVVFWEGDTESNLY
                     YLQYGSLKALRTAPDGREQVLRFLDAGEIFNEIGVLAKRPNPVTAVALEESGIWLIPR
                     HALEEVVMKYPQTALQIIGNMADKIISLVTLAADLSLKTVEARFAKLLLDSTEGDVIE
                     RRRWTNQTELAAHLGTVTDVLSRVIRELTKAGLIEMDKQHIRILNRASLAERAMLQED
                     "
BASE COUNT          884 a          770 c          675 g          855 t
ORIGIN      
        1 ggaattgcgg ttttggacct tctgccgaaa agacagcttt cgattttgta tctgtgaaat
       61 agatgtcgga catatcgtct cctttgatat gcctgacttt actggaagtc cccgcctact
      121 gccccgactt ttgtcgggtt tttcaaccca aacctcaatc tccccgaatc atggctcgct
      181 ccgccaaatt gatatcatta cacgagcgaa ctaccgaata atctctatta caatcaacct
      241 atcctcggaa gtaatcaact actgataatg cttttcactg catatggata aaccatgaat
      301 tctaaacaac caaggcatac tccgaccttc cgcgcatctc aattggctgt actaaacaag
      361 ctaatctcga tgactcatgc gtggaccgat acccaaacag tcttatcaat ggcattggag
      421 caagctgcac aactagcaat ggcagatggg gcagaatgtc atttggttaa ttcatctggg
      481 gaattgcagt ttacgactca gtacaactta gacatcgttt ttatgtccgg ctctttggat
      541 attcggtttc ccattgggaa aggcttacct ggtcttgcaa tttcaaacca aagcgctttt
      601 ttaatccctg atatcgaaaa cgaagaaaac tatcaacggc agcatctggc acagcaggcg
      661 cgttattgtt cgctgatatg tgtcccgctt tccggcatga actcacttct gggcacaatt
      721 atgctgtatt ttagaaaaag aattcaacca gatgctgttt tgctagaaat tctgactgcc
      781 attggacaac accttggtgt ttcaattgaa cgcgcgcatc tctttcagca aacctcagaa
      841 caattaaagg agttacaaat attgcagact gtggcaaatg ccttgaatcg ttctgcaaat
      901 attcaagaag ctttggaaca aagcttggag gctgttgtaa caaccatgaa tatgaggtgc
      961 ggctgggttg tactattgga tggtttccaa aagaaccgac ttgcggcgtc ctacaacctg
     1021 ccacccgaat tagaccctgc cgactggtct gccatgcgaa cccactgtcg ttgtattgag
     1081 ttgctgcaac tcggaaagct ggatgcagca atcaaaatcg ttgaatgtca acaactaaaa
     1141 aaagttaaga gtcccgatta cccttatcat tcccatgcca gtatacctgt tcgtgttgga
     1201 actttgctgt tgggcaatct caatatagtg cctccatcag gctctgccat tacgattgat
     1261 aattatcgac tcttcaactc cattggcgat cagattggag ttgcaattga gcgcgctcgc
     1321 ttgtatgaac aggcaaagga acagcgcact ctcgaacagc aaatcctgct cgggcatgga
     1381 caaatgcttt tgggagaacg caaactgcaa atcatcgtga accaaaccat aaaagtcgtt
     1441 tctaatgcgt tacgggttga atatgttaca ttggttttgg ttgcctcgga tggaaaaatt
     1501 tctctggaga ctgatttggg attctcgccc cggaaaacac gggatactgt tgatgttttg
     1561 cttgccgata actccgccat agttcaatct attcgattga agatgcccat cattaatcca
     1621 gacattaatc ttgaaagaca attgaaaaca aacttggtag atgaaaaaat aataatgaca
     1681 tcatcattga ttgttccaat gttaatgggc gaagaggcac taggcagcat tgcagtatat
     1741 tcccaatctc cccggcaatg gagcgaagat gagattcgtt tgctttccct gcttgccaac
     1801 cagacagcaa ttgccattga gaatacccgc ctgcttgaag ctgagcacac tgcccgtaaa
     1861 catgcagaaa ttctccactt gcaaacaata caacaagctc aagatctgac cctggcatac
     1921 gatacaacta tcgaaggctg gtctcgcgca ctcgatttgc gcgacaaaga aacagaagag
     1981 cataccttgc gcgttaccaa cctaactctt caattggctc aagctttcga tattagccat
     2041 gtcgaattaa aacatataaa gcatggtgca ttactccatg atattggaaa aatgggtata
     2101 cccgataata ttcttcgaaa agcaggagaa ctaagcgatg atgagtgggt aatgatgcgg
     2161 cagcatcctc aacttgctta tgaaatgctg tctccgattg catatttact acccgcgttg
     2221 gatattccgt attgtcatca cgaaaaatgg gatggtactg gctacccacg cgggctaaag
     2281 ggggaggaaa ttccgctggc tgcacgacta tttgccgtgg tggatgtctt tgatgcgctg
     2341 accagtgatc gcccatatcg ccctgcatgg gcaaaaaata aagctattga atatatccgc
     2401 caaaaggcag gttcacattt tgatccaagc gttgtagaag tgtttttaag attgattgat
     2461 aataaataat atgcaatata ctttgtctga ggtgctatct catagtgtcg ggttttcaac
     2521 ccaaacatta atcttcctga agcatggctc gctccgccaa gcttgcacgg ttcagaatac
     2581 ggatgtgttg tttatccatc tcgatcagcc ccgccttcgt caattcacga atgacacggc
     2641 taagtacatc ggtcacagtt cccaagtgtg ccgccagctc cgtctgattc gtccagcggc
     2701 ggcgttcaat tacatccccc tcggttgaat ccaataatag tttggcaaag cgtgcttcaa
     2761 ccgtctttaa cgacagatcc gccgcaagcg tgaccaggct gataatctta tctgccatgt
     2821 tgccaataat ctgcaacgcc gtctgcggat atttcatcac cacttcttct aacgcatggc
     2881 gtggaataag ccaaatgccc gactcttcca aagccaccgc cgtcaccggg ttgggacgtt
     2941 tcgccagcac gccgatctca ttgaagatct cgcctgcatc cagaaatcgt aatacctgtt
     3001 cgcgtccatc cggcgcggtt cgcaaagctt tcaacgaacc atattgcagg taatacaaat
     3061 tagactcagt atcaccctcc caaaatacga ccgcatgagg cggaaatact ttccacatgg
     3121 cactattggc aagagctgtc atggtttcat gatccagtcc gcgcaggaat tcaaaatctt
     3181 gcag
//
LOCUS       DAOQJE010000366         3183 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_19985_length_3183_cov_10.885550, whole genome shotgun
            sequence.
ACCESSION   DAOQJE010000366 DAOQJE010000000
VERSION     DAOQJE010000366.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3183)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3183)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3183
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19985_length_3183_cov_10.885550"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..704)
                     /locus_tag="PLT08_10185"
     CDS             complement(<1..704)
                     /locus_tag="PLT08_10185"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNE04876.1"
                     /translation="MKFKKVLRTAWEGLMLNKVRSFLTTLGVIIGVASVIVMLSVSAG
                     TEAAIAEQINALGANLIIVSPMRGVPGAGRTMMIADAYAIEEQVVGIDGISAEQGPAP
                     QIVKGNGITLESIAVIGTTADFPNVRDYAVASGAYFTADEDERKAKVVVLGAGIAKDL
                     FGDTSPLGQTITVGTVKLTVIGVMEAKGMVSDVDYDGRVYLPINIVFQKFMSEMPIGG
                     DRVRTIYVKADSQENIP"
     gene            complement(701..1399)
                     /locus_tag="PLT08_10190"
     CDS             complement(701..1399)
                     /locus_tag="PLT08_10190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNE04877.1"
                     /translation="MTKVYGMGEATVHALAGVDMTVEKGEFVAIMGHSGSGKSTLMNI
                     LGCLDRPTAGAYIFDGRDVSRLSKNELAEVRNQKLGFIFQSFNLLPRLSALSNVMLPM
                     LYNMEEDISDEDAEKRAAASLQAVGLGERLHHQPNQLSGGQQQRVAIARALVNNPPLI
                     LADEPTGNLDSRSSVEIMEILHHLHKHGATIVMVTHEPDIAEHAGRVVCVKDGKILSD
                     GHEGNNPCLNKRMA"
     gene            complement(1426..2739)
                     /locus_tag="PLT08_10195"
     CDS             complement(1426..2739)
                     /locus_tag="PLT08_10195"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HNE04878.1"
                     /translation="MKFFKNRSSLIIAVVVIAALIAGGIFYSQNTASAADGEETTVQT
                     AKVRKGDMVITASGAGVVEPSAQVDLAFRSTGVLHELNVEMGSQVTSHQVLARLEENI
                     QAEADFQALFSPSGVANAKLAVSNAQADLDAATNAYAYVVGSDAWYWISQAEEAQVVL
                     NALDASATQSQRDEAQSLFESARANRDYFLSLRTDIDELDMDLVTANLESAKVKLLDA
                     QTALEIVLAGPSTFTSPLAVIGQQTGKLEQARLAVEDTRLTAPFDGTVTELKVVNGQT
                     VNTTSVMTVSKTKDLFAHIYLDESDLDKVAKGNLVTITFDAYQDNPVIGEVILVEPAL
                     QVVDGSPVIAAWVALPSDMELSILPGMNLEAEVIAAESKDTLILPKQALRELEPGQYA
                     VFLVDTEGNLTLTAVEVGLTDYANAEILSGLKLGDVVSTGNVETK"
     gene            complement(2893..>3183)
                     /locus_tag="PLT08_10200"
     CDS             complement(2893..>3183)
                     /locus_tag="PLT08_10200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE04879.1"
                     /translation="PQALIYGWVLQFGIAVIPYIARRFFLKEETPELGGCWGSLALAN
                     LGSVLIWVSIFSGTAKGIIYGIGFVLYALALIHPLKELTEITRTGLKKFEAA"
BASE COUNT          782 a          843 c          757 g          801 t
ORIGIN      
        1 ggaatgtttt cctgactatc tgcctttaca tagatcgtgc ggacacggtc gccgccaatc
       61 ggcatctctg acatgaactt ttggaaaacg atattgatgg gaaggtaaac ccgcccgtca
      121 taatctacat cagagaccat gcctttggct tccatcacac cgataacggt caacttgacg
      181 gtaccgacgg tgatcgtctg cccaagtgga cttgtatcgc cgaacaggtc tttggcaatg
      241 cctgcgccca agacaaccac ttttgctttt cgctcatctt catcggctgt aaagtaagca
      301 cctgaggcaa ccgcataatc acggacattg ggaaagtcgg cggtcgtgcc aatgaccgct
      361 atcgattcga gtgtaatgcc attgcctttg acgatctgcg gtgcaggtcc ttgttcggca
      421 gagatgccat cgatgccgac aacttgttct tcgatggcgt atgcatctgc gatcatcatc
      481 gtacgcccgg cgccggggac accgcgcatg ggagatacga tgatcaggtt tgcgcccaag
      541 gcgttgatct gttctgcgat ggcggcttcg gttccagcgc tgacggataa catcacgatc
      601 actgaggcaa cgccaataat gacacccaga gttgtgagga aggaacgcac tttgtttagc
      661 atgagtcctt cccaggcggt gcggagtacc tttttaaatt tcatgccatc ctcttgttca
      721 ggcagggatt attcccttcg tgtccatccg ataagatctt tccatccttt acacagacca
      781 cacgcccggc atgttcggca atatccggct catgggtcac catcacaatc gtcgcgccat
      841 gtttgtgaag gtggtgcaag atctccataa tctccacact ggaacgcgag tcgaggttgc
      901 cggtcggttc gtccgccaaa atgagcggag ggttattgac caatgcccgc gcaatggcaa
      961 cacgctgttg ctgcccgccc gaaagctgat tgggttgatg gtgcaatcgc tcgcccaacc
     1021 caacggcttg caatgaggcg gcagcgcgtt tttctgcgtc ttcatcactg atatcttctt
     1081 ccatgttgta cagcatcggc agcatcacat tcgagagggc gctcaaacgc ggaagtagat
     1141 tgaacgattg aaaaataaac cctaatttct gattgcgaac ttcagccaat tcatttttag
     1201 acaatcggct tacatcccgc ccgtcaaaga tatacgctcc ggctgtaggg cggtcgaggc
     1261 agccgagaat gttcatcaag gtggatttcc ccgaaccgga atgccccatg atggcaacga
     1321 actcaccttt ctccacggtc atatccacac cagcgagggc atgtacagtg gcttcgccca
     1381 ttccgtacac tttcgtcatg ccttgaattt cgatcagcgg cttgctcatt ttgtctcaac
     1441 attccccgtg ctgaccacgt cacccagctt gagtccgctc aatatttcag cattggcata
     1501 atcggtcaac ccaacttcaa ccgccgtgag ggtcaggttg ccttctgtat ccacgagaaa
     1561 gactgcatat tgacccggtt ccagttcacg cagtgcctgt ttgggaagga tcaacgtgtc
     1621 ttttgattct gctgcgatca cttctgcttc aaggttcatg cccggtaaga tcgagagttc
     1681 catgtccgaa ggcaatgcaa cccatgctgc gatcaccggt gatccatcca caacctgtaa
     1741 agcaggctct accagaatca cttcaccaat cacagggtta tcctgatagg catcgaaagt
     1801 gatggttaca agattacctt ttgcgacctt atccagatca gactcatcca gataaatgtg
     1861 tgcaaacaga tctttcgttt tagaaactgt cattaccgat gtagtattta ctgtctgtcc
     1921 atttacgacc ttcaactcgg tcacagtgcc gtcaaacgga gcagtgagac gtgtatcttc
     1981 cacggcgagg cgagcttgtt ccaacttccc agtttgctgt ccgatcacag ccaacggcga
     2041 ggtaaaagtc gagggtcctg ccaacacaat ttccagtgcg gtttgagcgt cgagcaattt
     2101 gacctttgcc gattcaaggt tcgctgtcac aaggtccata tccaattcgt caatatcagt
     2161 gcgaaggctc aggaagtaat cacgatttgc acgagcggat tcgaacagac tttgcgcttc
     2221 atctctttga ctttgcgttg ctgacgcatc cagggcattg aggaccacct gggcttcttc
     2281 tgcctgtgaa atccaatacc aggcatcact gccgactaca taagcgtagg cattcgttgc
     2341 ggcatcaagg tctgcttggg cattgctcac tgccagcttt gcattggcga ctccggatgg
     2401 actgaacaac gcttgaaaat ctgcctcggc ttggatattc tcttccaaac gcgccaacac
     2461 ctgatgcgac gtgacttgtg aacccatttc aacattcagt tcgtgcaaca cacctgttga
     2521 gcgaaaagca agatcgacct gtgctgacgg ttcgacaaca cccgcgccac tggctgtgat
     2581 caccatatcg cccttgcgca ctttggcggt ttggacggtt gtttcttcac catccgctgc
     2641 gctggctgtg ttttgagaat agaaaatacc gcctgcaatc aaagctgcaa taacaactac
     2701 agcgattatg agacttgaac gatttttgaa gaacttcaag gttagctcct taaacttgcc
     2761 tgagatgacc cgatgataaa tattccccat aaagatagat tgtttgaaat gtaaagaatt
     2821 gataaaggaa gaaatgggga attaaaagca aaaaggcatg ggagagataa cccatgcctt
     2881 tgttttgtaa aactacgccg cttcaaactt tttcaagcca gtacgtgtga tctcggtcaa
     2941 ctctttcagt gggtggatca atgctagtgc gtaaaggaca aagccaatgc catagataat
     3001 gcccttggca gtaccgctaa aaatacttac ccagatcaac acactgccaa ggtttgccag
     3061 cgcaaggctg ccccagcatc cacccagttc gggagtctct tctttgagga agaagcgccg
     3121 ggcaatgtat gggatgacgg caatgccaaa ttgcagcacc cagccataga tcaaggcttg
     3181 agg
//
LOCUS       DAOQJE010000367         3183 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_19986_length_3183_cov_7.859655, whole genome shotgun sequence.
ACCESSION   DAOQJE010000367 DAOQJE010000000
VERSION     DAOQJE010000367.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3183)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3183)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3183
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19986_length_3183_cov_7.859655"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..500
                     /locus_tag="PLT08_10205"
     CDS             <1..500
                     /locus_tag="PLT08_10205"
                     /inference="COORDINATES: protein motif:HMM:NF024676.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="WYL domain-containing protein"
                     /protein_id="HNE04880.1"
                     /translation="DNEFRKLGRTIEFPNLIDTLLLARDYYELPSYSLDQLSQDFHIS
                     ANMQGSHALADAIVMKNLFFTMADSLKTIDKTLDDLIGIYNSPAWPEEGIYLPTELSE
                     AIHSGKKLFIQYMDKDGEISERWVSPREVIGLSDYLYLQAYCHSREADRTFRLDRILS
                     VEAEK"
     gene            complement(531..1079)
                     /locus_tag="PLT08_10210"
                     /pseudo
     CDS             complement(531..1079)
                     /locus_tag="PLT08_10210"
                     /inference="COORDINATES: protein motif:HMM:NF013488.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family oxidoreductase"
     gene            complement(1198..2793)
                     /locus_tag="PLT08_10215"
     CDS             complement(1198..2793)
                     /locus_tag="PLT08_10215"
                     /inference="COORDINATES: protein motif:HMM:NF025150.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4173 domain-containing protein"
                     /protein_id="HNE04881.1"
                     /translation="MKNNPNKLWIFVLALGWLFDFLFWKQAPGVNFAIFWTACLIVGF
                     YLLLSNGHRPHRTTLLLIPLFGFFAAVTFIRAEPMTTFLAYTFTMFTLTIFTVTYLGG
                     RWVQYALADYVGKLLSLVGSMLIRPITFTADVRKTQAEAGTQPSKYNFMPIVRGLVIA
                     IPIVMIFASLLASADVVFNQRLDDFVQIFKLENLPEYIFRMIYILVIGYALAGIFLHA
                     STESKDEKLVGEDKPVIPAFLGFIESSIVLGSVIALFAAFVVIQFQYFFGGTTNINVE
                     GYTYAEYARKGFGELVAVAFFALMMLLTLTGVTKRETETQRKTYSGLGIALVALLLVM
                     LVSAYQRLNLYEAAYGFSRLRTYTHVFLIWIGLLLITTIVLEILRKERMFTFAMLIAS
                     FGFAASLPILNVDAFIVNQNIQRELSGNDVEDLDSQYFIELSDDAVPPLVKALQTPSL
                     PDSVHEKVTAALACIRYQRDLQRNDREYSWQSFQFSRLNADNALKSVQAKIDVFELDE
                     SEYPTKAIAPSGEEYPCSQYYYD"
BASE COUNT          868 a          676 c          841 g          798 t
ORIGIN      
        1 tggataatga atttcgcaaa ctcgggcgga cgattgaatt tcccaacttg attgacactt
       61 tgctattagc tcgcgattat tacgaattgc cttcttatag cctcgatcag ctttcacagg
      121 attttcatat ctctgccaac atgcagggat cacacgccct agcagatgcg attgtgatga
      181 agaatctctt ctttactatg gcagattcgc taaagacgat cgataagacc ctcgatgacc
      241 tgattggtat ctataactcg cctgcctggc ccgaagaagg gatttacctt ccaactgaat
      301 taagcgaagc tatccatagt ggaaagaaat tattcattca atatatggat aaagatggcg
      361 agatatcaga gcgttgggtt tcgccacgcg aagtgattgg gttgtcagat tatttatacc
      421 tacaagccta ttgccactcc cgcgaagcag atcgcacctt tcggctggat cgaattttga
      481 gcgtagaagc ggagaaataa ataatgcgac ccgttcgggt cgcattattt ttatgctttg
      541 aagatttgtg ctaacgcgtt cttgaacgct tcatatggct gcgcaccaac aatagcgtat
      601 ttgtcgttga taacataggt ggggacaccg gttacgccga tttgttgtgc ccaattgacc
      661 tgcccagcaa cctctttggt gtacttgccg ccttctacaa ccgtttgcat atcgtctggg
      721 tctaagccag cttcttctgc tgtagcgcgg agcacatccc attgagctgg gtcgaagcca
      781 tcagcataga ccttgcggaa catgatgtga tggaactcat tggctttgcc atgttcgcgg
      841 gcatattctg tcgcttcgtg ggcgagacgt gtgttgtaaa ttcggtctgt agacctaaat
      901 ggcataccgt gcatttcagc catcattcgc aggcgttctt ccgagccatt gtcacgtgct
      961 cgtttgatgt aatcaggcaa ttccatgcct tcgggcggag tatcggggcg taaatagtac
     1021 ggacgccatt ccacctccac gttatattct ttcttaagcc tctcgaccac accctggccg
     1081 acatagcacc agggtcatat gtaatcggag aagatggtta gtttgaagtc cattggatgt
     1141 ccttttgtgt gattgatgat tgacagacat tcaatgaaac agcattctta cctagcatta
     1201 atcgtagtaa tactgtgagc agggatattc ttcgccgctt ggtgcgattg cctttgtggg
     1261 gtattcagat tcgtcgagtt cgaatacgtc aattttggct tggacagatt tcagagcatt
     1321 atctgcattg aggcgcgaaa attggaagga ctgccatgaa tactcgcggt cattacgctg
     1381 caaatcgcgc tggtagcgga tgcaggctag ggcggcggtc actttctcat ggacggagtc
     1441 aggcagggac ggagtctgaa gcgctttgac gagaggtgga acggcatcat cggagagttc
     1501 aatgaagtat tgagagtcca aatcttctac gtcatttcca cttagttcgc gttggatatt
     1561 ttggttgacg ataaaggcat cgacattcaa gatgggcaga gaggctgcaa agccaaacga
     1621 tgcaatcagc attgcaaaag tgaacatacg ctctttgcgc aaaatttcaa gcacgatggt
     1681 ggtgatgagc agtaagccga tccagattag gaagacgtgg gtgtaagtgc gcaagcgtga
     1741 gaagccataa gcggcttcgt agaggttgag gcgttgatat gcagagacca acatcaccag
     1801 caaaagtgca acgagtgcaa tgccaagccc agaataagtt ttgcgctggg tttctgtttc
     1861 gcgttttgtg acgccagtga gggtgagcag catcatcaaa gcaaaaaatg ctacagctac
     1921 gagttcgcca aaacctttgc gggcgtattc ggcataggta tagccttcca cattgatgtt
     1981 ggtcgtgccg ccaaagaaat actggaattg aatgaccaca aacgctgcga agagtgcgat
     2041 gacactgcca agtacgattg acgactcgat gaaaccaagg aaagcaggga tgacgggttt
     2101 atcttcgccg actagctttt catcttttga ttcggtggag gcatggagga aaatacctgc
     2161 caaggcatag ccgatgacca gaatgtagat catgcggaag atgtattcgg gcagattctc
     2221 tagtttaaag atttgcacaa aatcatcgag acgctggttg aagaccacat cggcagatgc
     2281 cagaagcgag gcgaagatca tcacgatcgg aatggcaatc acaagtccgc gtacgatggg
     2341 catgaaattg tatttggaag gttgtgtgcc tgcttcggct tgggttttac gtacatcggc
     2401 agtaaaggtg atgggacgaa taagcatact gccgacaagt gaaagtaact ttccgacata
     2461 gtctgccaac gcatattgga cccaacgccc gccaaggtaa gtgaccgtga aaatggtcag
     2521 ggtgaacatg gtgaaggtgt aagcaaggaa ggtggtcatt ggttcggcgc ggatgaaggt
     2581 gacagcggcg aagaagccaa agagtggaat cagcaataag gtcgtgcgat gcgggcgatg
     2641 tccattgctg agtaagaggt agaaccctac gatgagacat gctgtccaaa agatggcgaa
     2701 gttgacgcca ggagcttgct tccagaagag gaagtcgaat agccagccaa gggcaaggac
     2761 aaagatccag agtttattag ggttattttt cataagggct ccaaattatt tttaccacga
     2821 tatagggaag cggtaaaaag tttccccgag gatagccctg caaggcttgc ggagactctc
     2881 aaaaggtatc agaaatttga aaattgtggc aaattacaga gagcggcaaa ggcgaaaatt
     2941 acagaaatgt gagaatcgaa aatcgcccta ttgacatccg atttgattta tgacatactt
     3001 ttgctcacca gacgggggca aacaaattga attcgccatc cttatgaaag gaggaggtga
     3061 tgtctaacat ggatagtagt agtaaaccca gcgctgtggc atccctcctc aagtaagtca
     3121 aaggaagcca cagcgcccca cgattgccac ccgcaagggt ggcagtctgg tttaaaagag
     3181 tag
//
LOCUS       DAOQJE010000368         3181 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_20007_length_3181_cov_7.348688, whole genome shotgun sequence.
ACCESSION   DAOQJE010000368 DAOQJE010000000
VERSION     DAOQJE010000368.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3181)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3181)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3181
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20007_length_3181_cov_7.348688"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..656
                     /locus_tag="PLT08_10220"
     CDS             <1..656
                     /locus_tag="PLT08_10220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HNE04882.1"
                     /translation="EGAKALLDEAGIVDSNGDGNREYNGQELHYVATCPNGWSDWQAA
                     IEVVAAAGKEIGIDITTNYPEWSVYQTVVTKSDTPLPEGYDIFMMWSDGAGPTQPWSR
                     IRHLISSEFVGDAVNWNGNWGQYSNPAADELINAIPNESDPAKLNEMYTELVKIYLTD
                     VPSFTLMYRPQAFHTVNESVWTNFPHGEDGTNPPVPPLDLTDGWSIAGLYNLTLVNP"
     gene            926..1933
                     /locus_tag="PLT08_10225"
     CDS             926..1933
                     /locus_tag="PLT08_10225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNE04883.1"
                     /translation="MLKGYLKYFLNKLGWFAITFLFAFILNFLLPRLMPGDPVAAIVS
                     RLAQGMSNTSGVQAIYEQYTELFGTNKPMIEQFLIYVKNVARGDFGFSFSQYPRTVSD
                     VIGASIWWTLALQFPAIIVGWIIGNTLGALAAYLKGGFDKILMPISIFVSNFPAFGMA
                     VIFMVIFGVNLKWFPTSGGYGFDMIPNFSWAFIKSVIIHYQLPFWSIVVIAIGGQAIG
                     MRSMSIYELNADYVKYARFMGIRDSKIVGYVFRNAMLPQVTGLALSVGTMVGGALVAE
                     IVFSYPGLGTTLLRAVLGQDYPLISASTLIITVMALFANFIIEILYGLIDPRIKAAQS
                     D"
     gene            1948..>3181
                     /locus_tag="PLT08_10230"
     CDS             1948..>3181
                     /locus_tag="PLT08_10230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNE04884.1"
                     /translation="MKHIIQQVFRSGKFVTGFSIFSAIILIVIFYPLIITDQPLAIIG
                     QGTFFPPGIYVSVYDSMSANAYTLNLDEAAEMRIASKLNDEDRLAVKEWLVAAGIPES
                     DIDTTDTAKLLDQWFANYDSKKNIPGMTNAKRNYYIRLNNALKGLLSAEAATVMQTSP
                     QTNELEQIDTIQQTDYVNVGEVANVRVLPLGTDNFGRDVLTELVSATRVSLQIGLVAG
                     IIATIIGLTLGLLAGYIGGLVDDLIMFVTNLFIVIPPFVLLILISFSIGQEQRGAITV
                     AVVIGLTSWVWTARAVRSQVISLRNRDHVNLSKLSGHSIVHIILNDILPYIMSYVIMA
                     LILQISSGILAEAGLSILGLGPRTTEVPTLGLMMNWAMIYQAHILGNWWAYMPVLVTI
                     ALITFSMNLMNTGMDQVFN"
BASE COUNT          777 a          717 c          745 g          942 t
ORIGIN      
        1 tcgaaggcgc aaaagcgttg ctcgatgaag ctggtatcgt tgactccaat ggcgacggta
       61 accgcgaata caatggacag gaacttcact atgtagctac ctgccccaac ggttggtctg
      121 actggcaagc tgccattgaa gttgtggctg ctgctggtaa agaaatcggc atcgacatca
      181 ccaccaacta tcctgaatgg tctgtctatc aaaccgttgt aaccaagtct gacaccccac
      241 tccccgaagg ctacgacatc ttcatgatgt ggtctgatgg tgctggtcct acacagcctt
      301 ggtctcgtat ccgccacctc atcagctctg agttcgttgg tgatgcggtc aactggaacg
      361 gtaactgggg tcaatactcc aacccggcag ctgacgaatt gatcaacgca attccgaatg
      421 agagcgatcc tgccaagttg aacgaaatgt acaccgaatt ggtcaagatc taccttaccg
      481 atgtaccttc cttcacactc atgtaccgcc cgcaggcgtt ccacactgtg aacgaaagcg
      541 tatggaccaa cttcccgcat ggtgaagatg gcaccaaccc gcctgtgccc ccgctcgatc
      601 taacagacgg ctggagcatc gcaggtctct ataacctgac gctcgtcaac ccgtaatcaa
      661 gtttgtgttc aaaaacagcc atgttccatc ctgtatgggg catggctgtt cttgtaacca
      721 tcatgatgat aaaagcgtct accttcaaaa atcatccctt gctctgctta agaaattctt
      781 ctaagaacat ttcctctctt ttttagaaga gagtagggga ggttttttga cgagagacca
      841 agaaaactga agatagcaat ttaagtgaaa tctcttaaaa taagcgatag tagataaaag
      901 caacccgctg aatacatgga ggtgtgtgtt gaaaggatat ttgaagtatt ttctaaataa
      961 attggggtgg tttgccatta cgtttctctt tgcgtttatt ctaaatttcc ttcttccgcg
     1021 ccttatgccc ggtgaccctg ttgctgcgat tgtttcgcgt ttggcgcagg gaatgagtaa
     1081 cacctctggt gttcaggcaa tttatgagca gtacacagaa ttgttcggca cgaataaacc
     1141 gatgatcgaa caatttttga tttatgtaaa gaatgtcgct cgtggcgact tcggtttttc
     1201 tttcagccaa tatcctagaa cagtatctga tgtcattggt gcttccattt ggtggacact
     1261 tgctttgcag ttccctgcca ttattgtggg atggattatc ggaaatactt tgggcgcttt
     1321 agcagcttat cttaagggcg gttttgataa gatccttatg ccgattagca ttttcgtcag
     1381 taattttccg gcattcggta tggcggttat tttcatggtt atttttgggg tcaacctcaa
     1441 atggttccct acctcaggtg gatatggttt cgatatgatt ccaaatttca gctgggcatt
     1501 tattaaatcg gtcattattc actatcagtt gccattctgg tcaattgtag tgattgccat
     1561 tggtggacag gcaattggta tgcggtctat gtctatttat gaattgaatg cagactatgt
     1621 gaagtatgcc cgctttatgg gtattcgtga tagcaagatc gttggctatg tgttccgaaa
     1681 tgctatgctt ccgcaggtga ccggtttggc attgtcggtt ggaacaatgg ttggcggtgc
     1741 acttgttgct gaaattgttt tcagttaccc cgggcttggc acaaccctgt tgcgagctgt
     1801 gctaggacag gactatccat tgatctccgc atcaaccttg atcatcaccg ttatggcatt
     1861 atttgccaat ttcattattg agatcttgta tggcttgatc gatccgcgca tcaaagctgc
     1921 acaatctgac taagtggaga aaccagcatg aaacatataa tccaacaggt ttttcgctcg
     1981 ggtaagttcg ttacaggctt ctctattttt tcagccatca ttctgattgt gatcttttat
     2041 ccattgatca ttaccgatca gcctttggca attattggtc aaggtacgtt ctttcctcca
     2101 ggaatttacg taagtgtata cgatagtatg agcgcgaacg catacacact caacttagat
     2161 gaagcagctg aaatgcgtat tgctagtaaa ctaaatgacg aagatcgtct ggctgtaaaa
     2221 gaatggttag tcgctgctgg cattccagaa agcgacattg atactacgga tacggcaaaa
     2281 cttctggatc agtggttcgc aaattacgac tcgaaaaaga acatccccgg catgacgaat
     2341 gctaaacgta attactacat tcgcctcaac aacgccctta aagggttgct ttctgctgaa
     2401 gcggcgacgg tgatgcagac cagtccgcag accaatgaac tagagcagat tgatactatt
     2461 caacagacag attatgtgaa tgtcggcgaa gttgccaatg tgagagtgct accgcttggt
     2521 acagataact ttggcagaga tgtgctaact gagctggtct ctgcaacccg tgtctctttg
     2581 cagattggtc ttgtggcagg aataattgcc accattatcg gtctgacctt gggtttgctc
     2641 gcgggctata tcggcgggtt ggtagatgac ctcatcatgt ttgtcacgaa cctttttatt
     2701 gtcatcccgc cgttcgtgtt attgatcctc atctcattta gtattgggca agagcaacgt
     2761 ggcgcaatta ctgttgcagt agtgatcggg cttacctcat gggtgtggac agcacgtgct
     2821 gtccgttcgc aggtcatttc tttgcgaaac cgcgaccatg tcaatctttc aaaactctct
     2881 gggcattcca ttgtccacat cattctcaat gatatcttgc catacatcat gtcatatgtg
     2941 atcatggcgc tcattttgca aatctcctct ggtattttgg cagaggcggg gctttctatc
     3001 cttgggttgg gaccgcgcac tacggaagtc cccactctgg gattgatgat gaactgggcg
     3061 atgatttatc aggcacatat tctcggcaat tggtgggctt atatgccggt gctcgttacc
     3121 atcgcgctga ttaccttctc gatgaacctg atgaataccg gtatggatca ggtcttcaac
     3181 c
//
LOCUS       DAOQJE010000369         3178 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_20052_length_3178_cov_6.711175, whole genome shotgun sequence.
ACCESSION   DAOQJE010000369 DAOQJE010000000
VERSION     DAOQJE010000369.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3178)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3178)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3178
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20052_length_3178_cov_6.711175"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..476)
                     /locus_tag="PLT08_10235"
     CDS             complement(<1..476)
                     /locus_tag="PLT08_10235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000086807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNE04885.1"
                     /translation="MAKTPSKALDPEAKPDDRVDNALRPQKLNDLIGQNQVKENLSIL
                     IAAARQRSEALDHVLFYGPPGLGKTTLAHVLGNEMGVNVKVTAGPAIERAGDLAAILT
                     NLRAGDILFIDEVHRLGRAVEEVLYPAMEDFALDIVIGKGPSARSIRLKLPRFTVVG"
     regulatory      complement(577..671)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
     regulatory      complement(751..837)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
     gene            complement(875..2002)
                     /locus_tag="PLT08_10240"
     CDS             complement(875..2002)
                     /locus_tag="PLT08_10240"
                     /inference="COORDINATES: protein motif:HMM:NF027890.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family efflux transporter periplasmic
                     adaptor subunit"
                     /protein_id="HNE04886.1"
                     /translation="MKKNTLLFITLIVLLTLVLSACGGGQQTDATQEPVANVINASDV
                     VAEGRLEPAHATNLTFQARGVVEDVLVKVGDTVSEGDVLARLANAGAAEAQVVVAQNA
                     YDTLLRNESGDRAKLWEAYMNAQIVRAEAEKKWEDVDEDAVKNRIEDINAEIEDLKDT
                     LKDAQDEFDKYKDLDEDNSKRKAADDDLKTAQDNLNAKIRELEEETRSRDEVKAAYDA
                     ALDAEAEAKYQYEISLDGPNADQLTLAKANLDAAKDALSNYVITAPFSGVVADVNVKA
                     GEQVGTDTRVVSIIDDSQWFVKTTDVTELEVVSLSIGQAVTIRPDALTDLEMTGTVTE
                     ISSAYTQQGGDILYTVRVRVDGSDPRLKWGMTVETVFRTGE"
     gene            complement(2048..>3178)
                     /locus_tag="PLT08_10245"
     CDS             complement(2048..>3178)
                     /locus_tag="PLT08_10245"
                     /inference="COORDINATES: protein motif:HMM:NF014717.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNE04887.1"
                     /translation="LRNTFRRTNRLLLTLFTLTMGGAIFIAVFNVRVSLRDYTQQIGS
                     YFRADVTLDFDTSYRMNEIRQFAFQMDGVEAVEGWQFIGVELLYPDGSVAENVNLLAP
                     PATTTLVKPMIAAGRWIQPGDVRKIAISEAVLKYYPDLQPGDSIYLKVDGQEEVWEVV
                     GIFKFVGREGVLAYTPLEYAEKITNLTDRAYSFRVVTTQHDRAFQDAMSEKLDDYFRE
                     KGFKIRQAQSGLASLDSVSESLDILVVFLLIMAVLTAIVGAMGLTGTMGMNVLERTRE
                     IGIMRAIGADDRAVMRTVIGEGFVIGAISFILAIIVSIPLTYGLSFIVSIAIFETPIA
                     VLFTYQGYAIWLGLVLLLSVLGSILPARNAARLTIREVLAYE"
BASE COUNT          836 a          838 c          671 g          833 t
ORIGIN      
        1 cccactaccg taaaccgagg cagtttcagg cggatggaac gagccgaagg tcccttgcca
       61 atgacaatat ccaaagcaaa atcttccata gctgggtaga gaacttcttc cacagcccgc
      121 ccaaggcgat ggacttcatc aataaagaga atatcgccag cacgtaaatt cgtgagaatg
      181 gcagccaaat caccagcacg ctcaatggcg ggtcctgccg tgactttgac attaaccccc
      241 atctcgttcc caagcacgtg tgcaagtgta gtctttccca aaccgggagg accgtaaaac
      301 aagacatgat ccaacgcttc actacgttga cgcgccgccg caatgagaat ggataaattc
      361 tcttttacct gattctgtcc aatcaggtca ttgagctttt gtgggcgcaa tgcgttatcc
      421 acgcggtcat cgggcttggc ttcagggtcg agtgctttgg aaggtgtttt tgccatgtgc
      481 gcgattatac cctttacagg tagcttgccc tgcaaacaat tagcttaaaa ccaagcaggg
      541 atgacctaaa atgtcatccc tgcaaacggt ttgctcttcg gcagtctggc gatcttgatc
      601 gtatatcaaa tcacgattgt agacccatga ctttgcgtcg ccgggtttcc ccgggtttgc
      661 ctttcggcag tcggtcggtc ttatccggta acagtaacca gaggtagacg ctctgacttt
      721 gcgccgccgt cttttaacgg ttttgctttt atcggcaacc ctcacactca tggctcagtt
      781 cagctttaga gtagggcttt gcgccgccgg gttttcccgg ttttgctctt tctcggagca
      841 attttattat tgcactcttt tcgtgcaatt tcaatcactc acctgttcta aaaacggttt
      901 caaccgtcat accccatttc aagcgtggat ctgaaccatc cacacgaacg cggacggtgt
      961 aaagaatatc gccgccttgt tgagtgtaag cactgctaat ctcggtaaca gtaccagtca
     1021 tttcaaggtc ggtcagagca tcaggacgaa tggtaactgc ctgaccaatg ctcaaactta
     1081 caacttccaa ttcagtcaca tccgtggtct tcacaaacca ttggctatca tcaatgatac
     1141 ttaccacacg agtatctgtg ccaacctgtt cgccagcctt gacgttcaca tctgctacaa
     1201 cgccagagaa tggagctgtg atgacatagt tagaaagggc atcttttgcc gcatcaaggt
     1261 ttgctttggc aagtgttagc tgatcagcat tgggaccatc caagctaatc tcatattgat
     1321 atttagcttc tgcttctgca tcaagagcag catcataagc cgctttcact tcgtcgcgtg
     1381 aacgggtttc ttcttccaac tcacgaatct tcgcattgag gttatcttgt gctgttttga
     1441 ggtcgtcatc agcagcctta cgcttgctat tatcttcatc aaggtctttg tacttatcga
     1501 attcgtcttg tgcgtctttc aacgtatcct ttaagtcttc gatctctgca ttgatatctt
     1561 caatacggtt cttaacagcg tcttcatcta catcttccca tttcttttcc gcttctgcgc
     1621 ggacgatttg ggcattcata tatgcttccc acaacttagc gcgatcacca ctctcgttac
     1681 ggagtaatgt atcgtaagca ttttgtgcga cgacaacctg cgcctctgcc gcgccggcat
     1741 ttgcgaggcg agccaaaaca tctccctcgc tgacagtatc cccaactttg acaagcacat
     1801 cttccacaac gccgcgtgcc tggaaagtca aattcgtggc atgagcaggt tcaagccgtc
     1861 cttcagcaac aacatcgctg gcattaatta cattggcaac aggttcttga gttgcatctg
     1921 tctgttgacc gccaccgcac gctgagagaa caagcgtaag gagcacgatc aaagttataa
     1981 aaagtagtgt atttttcttc atggaaacct tcttcaattt tcgattattg atctttatgt
     2041 agatttttta ttcgtacgct agaacttccc gaatggtcag tcgcgccgca ttacgagcag
     2101 gcaaaatcga accgagaacg gaaagcaata acaccagtcc aagccaaata gcatatccct
     2161 gatatgtaaa taggactgca ataggcgttt caaaaatggc aatactcaca ataaacgaaa
     2221 gtccatatgt aagtgggatg gaaactatga tcgctaaaat gaacgagatg gcgccgatca
     2281 caaagccttc tccaatgacc gttcgcatca cagcgcggtc atccgcgccg atggcacgca
     2341 taataccgat ctcgcgagta cgctctaaca cattcatacc catggtaccg gtcaagccca
     2401 ttgcgcccac aatagcggtc aatacagcca taatcaatag gaacacaact agaatatcca
     2461 agctttcaga cacagagtca agtgaagcta aaccagactg tgcctgacgg atcttgaagc
     2521 ctttctcgcg gaagtaatca tccagcttct cagacattgc atcctgaaat gctctgtcat
     2581 gttgagttgt cactacgcgg aatgaataag cccgatctgt caggtttgtg atcttttctg
     2641 catactccaa gggagtataa gcaaggacac cctcacgccc aacaaactta aagatgccca
     2701 ccacttccca aacttcttcc tgcccatcca ccttaagata aatagaatca cctggttgaa
     2761 ggtctggata atacttaagc accgcttcac taatagcgat cttgcgaaca tctcccggct
     2821 ggatccaacg ccccgctgca atcattggct tgaccaaggt cgttgtcgca ggcggcgcaa
     2881 gtaaattcac attttccgcc actgaaccat cagggtataa cagttcaacg ccaataaatt
     2941 gccagccttc cacagcttcc accccgtcca tttgaaaggc aaactgacgg atctcattca
     3001 ttcgatacga tgtatcaaaa tcaagagtga catctgcacg gaagtaactg ccgatttgtt
     3061 gggtatagtc acgcaaactg acacggacat tgaataccgc aataaaaatc gcgccaccca
     3121 tggttagcgt aaaaagggtc aataaaaggc gattcgtacg gcggaaggta ttccgcag
//
LOCUS       DAOQJE010000370         3178 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_20054_length_3178_cov_4.708293, whole genome shotgun sequence.
ACCESSION   DAOQJE010000370 DAOQJE010000000
VERSION     DAOQJE010000370.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3178)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3178)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3178
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20054_length_3178_cov_4.708293"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..246
                     /locus_tag="PLT08_10250"
     CDS             <1..246
                     /locus_tag="PLT08_10250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005834565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 125 protein"
                     /protein_id="HNE04888.1"
                     /translation="GMIWHLSIIMRALTSKDENEIIHCLSMLKHTHAGTGFMHESFDK
                     EEPSQFTRKWFAWANSLFGELILKTYNERPRILKMFL"
     gene            302..577
                     /locus_tag="PLT08_10255"
     CDS             302..577
                     /locus_tag="PLT08_10255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE04889.1"
                     /translation="MDNDLETMTKEELIAEVRKLRAGIRNHRDATGHELCWYHPDLWN
                     LLPEKVSPEILVPEWSKFMNGCIQYRKSLDEQAPNAPRVDTDFEDEK"
     gene            633..1430
                     /locus_tag="PLT08_10260"
     CDS             633..1430
                     /locus_tag="PLT08_10260"
                     /inference="COORDINATES: protein motif:HMM:NF013775.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HNE04890.1"
                     /translation="MTLEEIPLTGGNVNSNVVRIGNTVRRAMTKVSPTVHQLLLHLEA
                     KGFVGAPRFLGIDEQQREILSFIEGETTFSEALWQTDAPLVAAAQLLKNYHDATLDFP
                     KKDGLVWGITSPDPARHEVICHNDFAPYNFIFQAQSPYAVIDFDLVGPGPRLRDVAYA
                     AYWLTPLSFHRADLLPFTEADVKNDSRRLHLFCETYGIRASEALFDMVYEVLCFMGDK
                     TKMEEILGDEIAAKLEREGHVKHWQAEAQAFLENRNRFAKNLSQPDQ"
     gene            1441..1743
                     /locus_tag="PLT08_10265"
     CDS             1441..1743
                     /locus_tag="PLT08_10265"
                     /inference="COORDINATES: protein motif:HMM:NF015126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EF-Tu/IF-2/RF-3 family GTPase"
                     /protein_id="HNE04891.1"
                     /translation="MTTDPTFRMTVQDIFFIKGRGTVVTGRVESGIITVGDEIRIQGK
                     SANKTAIVTGVEVLRKVTTQAKAGDNIGLLFKDITKEDVQVGDILTGSDLDFTWKP"
     gene            1780..2391
                     /locus_tag="PLT08_10270"
     CDS             1780..2391
                     /locus_tag="PLT08_10270"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF012521.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HNE04892.1"
                     /translation="MKEEIHITFLRHGRSRADDENVHEGRYDSPLTDVGRSQVQARVQ
                     DFLSRQFHFDAVISSPLQRAHETATIIGQALSLPIETDADWMEMDNGPLAGMPWDEAE
                     KQYPKPAFRNPYEPFCGSGESDWEIYNRGAKAVEKIIRRGPGSYLVVAHGGILNSALR
                     TIIGTQPSINQQGITFGFGDTGYARFSYRPAQHLWYLLEFKME"
     gene            2420..2956
                     /locus_tag="PLT08_10275"
     CDS             2420..2956
                     /locus_tag="PLT08_10275"
                     /inference="COORDINATES: protein motif:HMM:NF012792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNE04893.1"
                     /translation="MPTTSLTHRLAKREDLIALKALMDAAISENQKAFLDESQIASSR
                     TIMGLDTQLIDDGTYFIVEIDGQLAGCGGWSRRATLYGGDQTPGRSAALLDPAKDPAR
                     IRAMYTHPNHVRKGIGKLIITLCEEAAKAEGFTKMELVATLSGEPLYRTCGFEAYENI
                     VDDRGGAGVPLLRMRKTL"
BASE COUNT          913 a          782 c          705 g          778 t
ORIGIN      
        1 ggcatgatct ggcacctgag cattatcatg cgcgccctaa caagcaagga tgaaaatgag
       61 atcattcatt gtctatccat gctcaaacat acacatgccg gaacaggttt catgcacgag
      121 tctttcgata aagaagagcc aagtcaattc actcgcaagt ggtttgcttg ggcaaactca
      181 ttattcggtg aattgattct aaagacatat aacgaacgtc cgcgcatttt gaagatgttc
      241 ttgtaacaac gcaaaagaca tcaaattcct ttttcacaag gaagccgttt caggagatac
      301 catggataac gatctagaaa caatgacgaa agaagaatta atagctgaag ttaggaaact
      361 tcgagccggt attcgaaatc atcgtgatgc aacagggcat gaattgtgtt ggtatcatcc
      421 agacctttgg aatcttctcc cagaaaaagt atcgccagag atactggttc cagaatggtc
      481 aaaattcatg aatggctgca ttcaatatag aaaaagctta gacgaacaag ccccaaatgc
      541 gccccgtgtc gatacagatt ttgaagatga aaaatagcct tcagtatctt tcgagtaatt
      601 gtgtacagta aacatatcac acaaaaattc aaatgacgct tgaagaaatc cccttaactg
      661 gcggcaatgt caattccaat gtggtgcgca ttgggaacac tgtgcggcgt gcaatgacca
      721 aggtcagccc aacagtacac caactgcttt tacatttgga agccaaaggc tttgtggggg
      781 ctccacgatt tttaggcatt gatgagcaac aacgcgaaat attatccttt atagaagggg
      841 aaactacgtt ctcagaagca ctttggcaaa cggacgctcc gctggttgcc gccgctcaac
      901 tcctgaagaa ttatcatgac gccaccttgg attttcccaa aaaagatggt ctggtgtggg
      961 gcatcacatc tcctgaccct gctcggcatg aagtcatctg tcataacgac tttgcccctt
     1021 ataatttcat cttccaagcc caatcgcctt atgcagtaat tgattttgat ctggtaggcc
     1081 cgggtcctcg attgcgagat gtggcatatg ccgcttattg gcttacaccc ttatcatttc
     1141 atcgtgctga cctgcttccc tttacagaag cggacgtaaa aaatgacagt cgccgtcttc
     1201 acctgttttg cgagacctat ggcatccgtg ccagcgaagc tctttttgac atggtctatg
     1261 aggttttatg ttttatgggt gacaaaacga aaatggaaga aattctaggg gatgaaattg
     1321 ccgcaaaact tgaacgagaa ggacatgtaa aacactggca agcagaagcc caagcttttc
     1381 tagaaaatcg aaatcgcttc gctaaaaatc tgtctcagcc cgatcaataa aggattcccc
     1441 atgaccacag accctacttt tcgcatgacc gtacaagata tcttctttat aaaaggacgc
     1501 ggaaccgttg taactggtcg cgttgaaagc ggaataatca cggtcggaga tgaaattcga
     1561 atccaaggga aaagcgccaa caaaacggct atagtaacgg gggtagaagt ccttcgcaaa
     1621 gtcacaacac aggcaaaagc tggtgacaat atcggactac tgttcaaaga catcacgaaa
     1681 gaagatgttc aagttgggga tatcctaaca gggtctgatt tggatttcac ctggaagcca
     1741 taaacggcat tatccgatct tgttattttg ggagtactca tgaaagaaga aatccatatc
     1801 accttcctgc gccacgggcg gtctcgcgcc gatgacgaaa acgttcacga aggccggtac
     1861 gactcacctc tcactgatgt agggcgttcc caagtccaag cccgcgtgca agattttctc
     1921 tcccgacaat ttcattttga tgcggttatt tcaagtccgt tgcaacgagc acatgaaacc
     1981 gccacgatca ttgggcaagc gttgagtctc cccattgaaa cggatgccga ttggatggaa
     2041 atggataatg gtcccttagc tggcatgcct tgggacgaag ccgaaaaaca atatcccaaa
     2101 cctgcatttc gaaatccata tgaaccgttt tgcggttcag gagaaagcga ctgggagatt
     2161 tacaatcgcg gagccaaagc tgtagaaaaa atcattcggc gcggacccgg aagttattta
     2221 gtcgtcgccc atggtggcat tctcaattct gctttacgga caattatcgg cacacaacca
     2281 tctatcaacc aacagggcat cacatttggt tttggtgata ctgggtacgc ccgctttagt
     2341 tatcgtcctg ctcaacatct atggtatctc ctcgaattca aaatggaata aatacactga
     2401 aattaaaaaa ggaagcccca tgcccacaac ctctctaacc cacagacttg ccaaacgaga
     2461 agacctgata gcccttaaag ccttaatgga cgccgctatc tcagaaaatc aaaaagcctt
     2521 tctcgatgaa agtcagatcg cttcgagccg taccattatg gggttggata cgcaactcat
     2581 cgatgacggc acctacttca tcgtcgaaat cgatggtcaa ctggcaggct gcggcgggtg
     2641 gagtcggcgt gccaccctgt atggtggtga ccaaacccca gggcgcagtg ccgccctgct
     2701 cgaccccgcc aaagacccgg ctcgtatccg cgccatgtac acgcatccta accacgtccg
     2761 caaaggaatc gggaaactca tcatcaccct atgtgaagaa gccgccaaag cagaaggctt
     2821 cacaaaaatg gaattggtcg ccaccttatc tggcgaacca ctgtatcgca cctgcggatt
     2881 cgaagcttac gaaaatatcg tcgacgatcg cggtggtgcg ggcgttccgc tcttgcgaat
     2941 gcgaaaaacg ctgtaaaaat tttgtattcc ttacctctac atcttacatg gcaagaatta
     3001 agactcaacc tcgccatccc ttataattaa aggatgcaat ttcgcctctc ccccataaca
     3061 cgtcaaacct tgctcattgc ggttatcacc atcatcgcct atgggttgct cttaccgctc
     3121 actggcttct actgggacga ctggcccttc gcatggatcg ccaaatttct tggtcctg
//
LOCUS       DAOQJE010000371         3173 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_20106_length_3173_cov_5.707505, whole genome shotgun sequence.
ACCESSION   DAOQJE010000371 DAOQJE010000000
VERSION     DAOQJE010000371.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3173)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3173)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3173
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20106_length_3173_cov_5.707505"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..605
                     /locus_tag="PLT08_10280"
     CDS             <1..605
                     /locus_tag="PLT08_10280"
                     /inference="COORDINATES: protein motif:HMM:NF012488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HNE04894.1"
                     /translation="TIIDGAHAPGQIPLHLDALGADFYGGNLHKWLCAPKGAGFLYAR
                     PEVQHLLKPLVVSWGYEAEAPGDSQFIDYNEWWGTRDISAFLAVPKAIQFQQDQNWAE
                     VRAACHRLAVETWQRIHDLTGETPLHSDPEPWFAQMAVATLPADSDLVALKSRLYDEH
                     RIEIPLIAWNEKKLIRLSVQGYNSRRDMDKLLSALNTLLR"
     gene            614..1630
                     /locus_tag="PLT08_10285"
     CDS             614..1630
                     /locus_tag="PLT08_10285"
                     /inference="COORDINATES: protein motif:HMM:NF012415.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAP domain-containing protein"
                     /protein_id="HNE04895.1"
                     /translation="MRKRISFLFALIILLVLSITPKQTVEAKPFANITSPSQLIDAVN
                     SLRVANGLSPLPVHQALMQSAQSQADYMAATGNVTHSRPSGTYTQQLLALGFPLAGDL
                     SAGGFRSENILSTYGPLVWNGVPSAWQDAQHMNTMLSGNFTHIGAGVSQSGDMYYYTV
                     DTAAATSSGQQQDSAATVLTSVPGGSSDAGVSQFMVPVTLNTPHPDGNVYHKVQYGQS
                     LWSIAIAYGTTIKNIQALNNLGEASTVYQGQELLVVTGATQAAAPTLDATATTQATAT
                     LAPTSTNVPTSTPELALLTPTEEAPVEANTKPASSRVLIVVLIVAAFVGAGMAVWLIR
                     DPGN"
     gene            1733..2611
                     /locus_tag="PLT08_10290"
     CDS             1733..2611
                     /locus_tag="PLT08_10290"
                     /inference="COORDINATES: protein motif:HMM:NF013862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HNE04896.1"
                     /translation="MEITLALGGGGAKGNAHIGVLRRLEKEGYKIRSVAGTSFGGMVA
                     ALYAIGYSPREIQAIFESVDQESLYGRASQDGPSLLGMAGVRQLLERVIGEKTFKDTR
                     IPCAVTAVDINTGGEVVLNEGSIKNAVLATIALPGIFPVQKINDWELVDGGVLNPVPV
                     SVARLLSPDLPVVAVVLNDPIDKPVRGYNLPLPSIVPRTIAERLARISLAQSLDIFLR
                     SVDVSSRYVSHLRLQVDKPEIIVRPDVHHIELLDQVVVEDVAALGEQALEQVLPDLKR
                     KTTWAARLGRRVFGAN"
     gene            2614..2853
                     /locus_tag="PLT08_10295"
     CDS             2614..2853
                     /locus_tag="PLT08_10295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE04897.1"
                     /translation="MTRDLRKYMRDTNTRIVVGAILMLFIIGLGLIWLIYGFGAAVTG
                     FLCIAAAFIPIGLVFLFLFGMDWFVKRANPDERND"
     gene            2825..>3173
                     /locus_tag="PLT08_10300"
     CDS             2825..>3173
                     /locus_tag="PLT08_10300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE04898.1"
                     /translation="MQTQTNETIEFNGWTMRYRSAETSHPRLLVMIHGLTGDENSMWV
                     FARRFSPNYWIIAPRAPHPANPQGFSWRANPLSIDEARANQTNRPKLDALKESAEGLI
                     RLIEEYAASVKVEA"
BASE COUNT          747 a          765 c          831 g          830 t
ORIGIN      
        1 tcactatcat cgatggcgct cacgcaccgg gtcaaattcc gcttcatttg gatgcacttg
       61 gtgcagactt ctatggcgga aatttgcata aatggctttg tgccccgaaa ggtgctggct
      121 ttttatatgc ccgcccagag gttcaacatc tgctcaagcc cttggtggtt tcgtggggct
      181 acgaagccga ggcgcccggt gattcgcaat ttattgatta caacgaatgg tggggtactc
      241 gtgatatctc agcgttcctt gccgtcccga aggcaattca atttcaacag gaccaaaact
      301 gggcggaggt aagagccgcg tgtcatcgcc tggcagtgga gacgtggcag cgcatccatg
      361 acttgacggg agaaaccccg cttcattccg accccgaacc ttggttcgcc caaatggcag
      421 ttgccaccct gccagcggac tctgacctcg ttgccctcaa atcgcgcttg tatgatgagc
      481 atcgtattga aattccgctc atcgcgtgga atgagaagaa attaatccgt ttgtctgtgc
      541 agggatataa ttctcgtcga gatatggaca aattattatc tgcattgaat acgttgttaa
      601 gatgaaagca tatatgagaa aacggatttc gtttttattc gccttgatta ttctgctcgt
      661 attgagcatt accccaaaac aaactgttga agcaaaacca tttgcaaata ttacatcccc
      721 atcccaattg atcgatgcgg tcaatagttt gcgggtcgca aatgggcttt cacccttgcc
      781 tgtccatcag gcattgatgc aatctgctca atcgcaagca gattacatgg cagctacagg
      841 caatgtaaca cactctcgcc ccagtggcac ctacacccag caattgcttg cgcttggctt
      901 tccgttagcc ggcgatctaa gtgcgggcgg ctttcgctcc gaaaatatct taagtaccta
      961 cggaccttta gtatggaatg gtgttccgag cgcttggcaa gatgctcaac atatgaacac
     1021 tatgctttcg ggcaacttta cacacattgg cgctggcgtt tcacaatcag gcgatatgta
     1081 ttattacact gtggataccg ccgctgcaac cagctcgggt caacaacaag atagtgctgc
     1141 aacggttctc accagcgtgc cgggcgggtc aagtgatgcg ggcgtcagtc agttcatggt
     1201 gccggtcaca ttgaatacgc ctcatcccga cggaaatgtg tatcataagg tgcagtatgg
     1261 gcaatcgttg tggagcattg ccattgccta tggcacaacc atcaagaaca ttcaagcgct
     1321 aaacaacctt ggcgaagcct caaccgtata tcaagggcaa gagttgctcg tggttacggg
     1381 cgccactcaa gcagcggctc caactttgga tgccacagct acgacacaag ccacggcaac
     1441 cttggctcca acttcaacca acgttcctac ctcaacccca gaactggcac tgcttacccc
     1501 tacagaagaa gcacccgttg aagcaaatac aaagccagcc tcttcaagag tgttgattgt
     1561 cgttttgatt gtggcggcat ttgtaggggc gggaatggca gtatggctga ttcgcgaccc
     1621 ggggaattaa gtttctcaga aaatgtgttg aaactatgtt gtttttttgt aagggtataa
     1681 tccctcgcgt ttgatttgtc cgttaattga atattgaaag gcatcgaaga aaatggaaat
     1741 tacgttggca ttaggcggtg ggggcgccaa gggcaatgct cacattggcg tgttacgtcg
     1801 tcttgaaaaa gaaggatata aaattcgctc tgtcgcaggg actagttttg gtggaatggt
     1861 cgctgcttta tatgctattg gctactctcc gcgagaaatt caagcaatct ttgaatcagt
     1921 agaccaggaa agcctttatg ggcgtgcctc gcaagatggt ccctctctat tgggaatggc
     1981 tggggttcgt caactgttag agcgtgtgat cggcgagaaa accttcaaag acacgcgcat
     2041 tccctgtgcc gtcaccgcag tggatatcaa cacgggcggt gaggtggtac ttaatgaagg
     2101 cagtatcaaa aatgctgtac tcgccacaat cgctttgccg ggtatcttcc cggtgcagaa
     2161 gatcaacgat tgggaactag tggatggcgg cgtcttgaac ccggtgccgg tgtctgtggc
     2221 tcggctctta tcgccagacc tgccggtggt tgccgttgtg ttgaacgacc ctattgataa
     2281 acccgtgcgc ggatataacc tgccgctgcc cagcattgtt ccacgcacga ttgctgaacg
     2341 gctcgcaagg atttcgttag cccagtcctt ggatatcttc ctgcgctctg tagatgtttc
     2401 cagccgctat gtttcgcatt tgcgcttgca agtggataag cccgagatca ttgtccgccc
     2461 ggatgtgcac catattgaat tgttagatca ggttgttgtg gaagatgtgg ctgccttagg
     2521 cgagcaggca ttagagcaag tattgccaga cctgaagcgc aaaaccacat gggctgcccg
     2581 tttgggtcgg cgtgtgtttg gagcaaatta acaatgactc gtgatctgcg caagtacatg
     2641 cgtgacacca atacgcggat cgtcgtcggc gcgattctga tgttgtttat catcgggctg
     2701 gggctgatct ggttgatcta cggctttggc gcagcagtga caggttttct ctgtattgcc
     2761 gcggcattca tcccgattgg tttggtcttt ttatttttat ttggaatgga ttggttcgta
     2821 aaacgtgcaa acccagacga acgaaacgat tgaatttaac ggttggacga tgcgttatcg
     2881 ctcagccgaa acctcgcatc cgcgtttgtt ggtgatgatc cacggtttga ccggcgacga
     2941 aaactccatg tgggtttttg cgcgccgttt ttctccaaat tattggatca tcgctccgcg
     3001 tgcgccacac cctgccaacc cgcagggttt ttcatggcgt gcaaatccgc ttagcattga
     3061 cgaagcgcgt gcgaatcaaa caaaccgccc aaagttggat gcattgaagg aatcggctga
     3121 agggttgata aggcttattg aggagtatgc agcttcggtt aaggtagagg cgg
//
LOCUS       DAOQJE010000372         3170 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_20130_length_3170_cov_6.217014, whole genome shotgun sequence.
ACCESSION   DAOQJE010000372 DAOQJE010000000
VERSION     DAOQJE010000372.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3170)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3170)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3170
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20130_length_3170_cov_6.217014"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..516)
                     /locus_tag="PLT08_10305"
     CDS             complement(<1..516)
                     /locus_tag="PLT08_10305"
                     /inference="COORDINATES: protein motif:HMM:NF017984.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0036297 - interstrand cross-link repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crosslink repair DNA glycosylase YcaQ family
                     protein"
                     /protein_id="HNE04899.1"
                     /translation="MDTISKQTYRRFLLGSQGLWPGRRFSGLSGTESALRQMQALQLD
                     PLVMVARSQDIAMYGRVLDYKPAMLYKMAYEKRKAFDYGGWLMMYPMQEFPYWQLHMQ
                     RRKEQGLRWEHFTHPPKQVVKFVLDELREKGPLGNRDIEGDAVKAWSYRGRKETSITL
                     FYLWLVGEVMIS"
     gene            complement(598..789)
                     /locus_tag="PLT08_10310"
     CDS             complement(598..789)
                     /locus_tag="PLT08_10310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE04900.1"
                     /translation="MKNTRLLLGGALIGAIIGLVAALLIGRRAEKNESETAITASEGV
                     KLGVLVFGLLRAISSLGDD"
     gene            complement(786..1235)
                     /locus_tag="PLT08_10315"
     CDS             complement(786..1235)
                     /locus_tag="PLT08_10315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE04901.1"
                     /translation="MSDKLPDLAERLKSKPAHYSYMKHRNQVMRQVILPVALSALVLV
                     GIVVLVSYATFNQGGDVGRWAAISTIWVVIPMLLGGLVVLAILGGLIYGMARLLGALP
                     YYTGIAQDCIFIARGYIIRGANMVVKPINALNDWLQTAKAFFERMTP"
     gene            complement(1304..2479)
                     /gene="ftsZ"
                     /locus_tag="PLT08_10320"
     CDS             complement(1304..2479)
                     /gene="ftsZ"
                     /locus_tag="PLT08_10320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HNE04902.1"
                     /translation="MNPTNKNVQTEAFARIKVIGVGGAGQNAVNRMMEEGIQGVEFIA
                     ANSDAQALTLSRAPIRVRLGDKITRGLGAGGDPEVGRKAAEESSDELYNVLKGADMVF
                     VTAGMGGGTGTGAAPVVSQIAKECGALTIGVVTRPFTFEGGKRSQSAESGVAKMKEHA
                     HTLISIPNDRLLQLADKKSSLQDAFRMADEVLHQGIQGISELITIPGLINLDFADVRS
                     IMSEGGAALMAVGRGSGDDRAKVAAEQAISSQLLDITIDGARGVLFNVTGGPNMTLFE
                     VNQAAAIIRETAHPDVNMIFGAVIDPNMGDEIRCTVIATGFERSGATRRVLERLPRTN
                     LSEKPVSVANTPFQTRPTESVNAPVPETRSSADVKSQPLPSINNDDLDVPTFLRNRR"
     gene            complement(2611..>3170)
                     /locus_tag="PLT08_10325"
     CDS             complement(2611..>3170)
                     /locus_tag="PLT08_10325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="cell division FtsA domain-containing protein"
                     /protein_id="HNE04903.1"
                     /translation="VGGNHITQDIAHGLRLSPSQAEEVKKQQGHALLSVIGPDEYFLM
                     RPFGEDKDVRISRQDLAHIIEARVEETFNLILQEIKRSGYDGLLPAGMVLTGGTSALL
                     GINRVASEVLDMPVRVAQPENLKGLVDKLNSPAYSTSFGLLQWAISMHDHQVTVSGGA
                     KRRKPRGEGNVKFDAIKDWMKRLLP"
BASE COUNT          801 a          819 c          792 g          758 t
ORIGIN      
        1 agaaatcatc acctcaccca ccagccagag ataaaacaag gtgatggatg tttccttacg
       61 cccacggtag ctccatgctt tgacggcatc gccttcaata tcacgattcc caagcggacc
      121 cttttcgcgc agctcgtcca acacgaactt aacgacttgt ttaggtgggt gcgtgaaatg
      181 ttcccagcgc aaaccctgtt ctttacgtcg ctgcatgtgc agttgccagt atggaaactc
      241 ctgcatgggg tacatcatca accagccgcc gtaatcgaag gcttttcgtt tttcatatgc
      301 cattttgtat agcatagcgg gtttgtaatc gaggacacgt ccatacatgg ctatatcctg
      361 actgcgagca accatcacaa gtgggtcaag ctgcaacgcc tgcatctggc gcaaagcgga
      421 ttcggtgcca gaaaggcccg agaaacggcg cccaggccaa agtccctgtg agccgagaag
      481 aaagcggcgg tatgtttgct tggagatagt atccatggtt aagagtctac agtcaaaggt
      541 caagataaaa aagaccgagg caggaacctc ggtctgtggt caatcgttta tcgtctgtta
      601 atcgtcgcca agggaagaga ttgcgcgaag caagccaaat accagcacgc ccaatttgac
      661 gccttcactt gcagtgatgg cagtttcgct ctcgttcttt tcggcgcggc gaccgatgag
      721 caaagcggca accagcccga taatagcgcc gatgagtgcg ccgcctaaaa gtagtcttgt
      781 atttttcatg gagtcatcct ttcaaagaag gcttttgcag tttgaagcca atcgttcaga
      841 gcgttaatgg gtttgacgac catattcgct ccgcgaatga tatagccgcg tgcgataaaa
      901 atacaatctt gggcaatgcc ggtgtaatat ggcaacgcac caagtaaacg agccatgcca
      961 tagatcaagc cgccaaggat ggcaagtacc accaacccgc cgagcaacat ggggatcaca
     1021 acccaaatcg tggagatggc agcccagcgc cccacatcac caccctggtt gaaggtggca
     1081 taactcacca acactacaat tccaaccaat accaaggcac tcaatgccac tggcaagatg
     1141 acttgcctca ttacctgatt acggtgcttc atgtaagagt aatgggcggg tttgcttttt
     1201 agacgttcag cgagatcggg gagtttatcg ctcataaatc tattttacca aaataaaaga
     1261 ccctaaaggt ttgcaacctt tagggtcttt gcttgtttag tgattatcgg cgatttctca
     1321 agaaagtcgg gacgtcaaga tcgtcgttgt tgatgcttgg caagggctga gacttcacgt
     1381 ctgctgagga acgggtttca ggaactggag catttaccga ttcagtgggg cgagtctgga
     1441 aaggagtgtt cgccacagag acgggtttct cgctcaggtt tgtgcggggc aagcgctcga
     1501 gaacgcgacg agtagcgccg ctgcgttcga agccggtggc aatcactgta cagcggattt
     1561 cgtcgcccat gttcgggtcg atcactgcac cgaagatcat gtttacatcg gggtgagctg
     1621 tctcgcgaat gatcgcagcc gcttggttga cttcgaagag cgtcatgttc ggtccgccgg
     1681 tgacgttgaa gagcacgcca cgagcaccgt cgatggtgat atcgagcaat tggctggaga
     1741 tggcttgttc agcagccacc ttggcgcggt catcgccaga gccgcgacct actgccataa
     1801 gagccgcgcc gccttcagac atgatcgagc gcacatcggc aaagtcgagg ttgatcaagc
     1861 cagggatcgt gatcaactcg gagatacctt gaataccctg atgcaacact tcgtccgcca
     1921 tgcggaaggc atcttgcagg gatgatttct tatcagccag ttggagcaaa cgatcattcg
     1981 gaatggaaat gagcgtgtgc gcatgttctt tcatcttcgc aacaccagac tcagcggact
     2041 gtgaacgttt gccaccttca aaggtgaagg gacgagtcac aacgccaatg gtgagagcgc
     2101 cgcattcttt ggcgatctgt gaaacgacag gcgccgcacc ggttccggta ccgccgccca
     2161 tgcctgcagt gacgaagacc atgtctgcgc ctttgagcac attgtagagt tcgtctgagg
     2221 attcttctgc agctttgcga cccacctcag ggtcaccgcc tgcgccgaga ccacgcgtga
     2281 tcttatcgcc gaggcgcaca cgaatgggag cgcgtgacag agtcaaggct tgggcatcag
     2341 agttggcagc aataaattca acgccctgaa taccttcctc catcatgcga ttaacggcat
     2401 tttgaccggc accaccaaca ccaatcactt tgatgcgggc aaaagcttcg gtctgtacat
     2461 ttttattagt cggattcatt tgttcctcct ggcaaggaat atgcctaatc ttaaacgaga
     2521 atacgttttt ttaacagggt taatttcgac caaaattcac actttgtgaa tcttttctca
     2581 tgatgtcaag ggttaaacag aatctcgtca tcatggtaac aacctcttca tccaatcttt
     2641 aatcgcgtca aatttgacgt ttccttcacc acgtggcttg cggcgtttag cgccaccaga
     2701 cacagtcact tgatgatcat gcatactgat cgcccattgc aacaaaccaa agcttgttga
     2761 ataagctggt gaattcaatt tatcgaccaa cccttttaag ttctcaggtt gagccacacg
     2821 aacgggcata tccaatactt cacttgcaac tcgattaatg cccaataaag cagaagtacc
     2881 gccagtcaac accatgccag cagggagcaa accatcatac ccagagcgct tgatctcttg
     2941 caagatgagg ttaaaggttt cttccacacg cgcttcgatg atatgagcca gatcttggcg
     3001 gctaatgcga acatctttat cttcaccgaa ggggcgcatc aggaagtatt catcgggtcc
     3061 gatcacagat agcagggcgt gcccttgctg ttttttcact tcctctgcct gagaaggtga
     3121 caaacgcaat ccgtgcgcaa tatcttgagt gatgtgattg ccacccactg
//
LOCUS       DAOQJE010000373         3160 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_20248_length_3160_cov_3.647665, whole genome shotgun sequence.
ACCESSION   DAOQJE010000373 DAOQJE010000000
VERSION     DAOQJE010000373.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3160)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3160)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3160
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20248_length_3160_cov_3.647665"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            238..2067
                     /locus_tag="PLT08_10330"
     CDS             238..2067
                     /locus_tag="PLT08_10330"
                     /inference="COORDINATES: protein motif:HMM:NF024700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNE04904.1"
                     /translation="MRIERITLTDYPPIKNIDIQISANLVIVAGANGSGKTRLKEAII
                     YPFATAHESQISLLLRSTTPDEESILGQEYLEINQGKISTTLRNYLQTRTGKNAFSGS
                     IIQIESNRTVRPVQFQSITSSTPDPDEQGLDHSLYLDNLSNRWNRLVNNLYAKSAIRN
                     DQLARFIKENPDKTGSDAAKAIPDPLEPFQKTFSNLFPDKKLEPIDIRFLREFEYRDK
                     NSQPLSFGTLSSGERELVTMVFDLIYKEISHSIIIIDEPELHLHPALTFRFIETLKGL
                     CNGTNQIILFTHSADLISTYYTNGNVFVLDPSSTNENQVHILNALNESHSTIANALSE
                     NLGIFAVGKNIVFVEGENSSIDRLIYGKVAQSGFSYFQIVPIGSVENIITLRSVINEL
                     RSTIFGVRLFLIRDRDGLSDKTISTLEENSCFRCLPRRHIENYLLDAEILRDVAISIG
                     MDAEKTSATNIERILQNIASQSLMSGVLWNVREYIRLQGSLSSPKINKGEINQIPPEK
                     LAETITLQLTSSFEELSDKFNSEAILKLTLEEYNKLQESLSSNEWKKYLPGKTIFNRF
                     CSDYFHEKPERIQEIYTNLALQKKPQVFQDISKIFQSFSELES"
     gene            complement(2056..>3160)
                     /locus_tag="PLT08_10335"
     CDS             complement(2056..>3160)
                     /locus_tag="PLT08_10335"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNE04905.1"
                     /translation="RIPSLTANSIQAMKVAQALIQLGHDIKMFAPKESPITNHQSLLT
                     HYGLRLAPELELLPSISRLKRLDFIVHAQWAAKKFGADLIYTWLPQSAVLGAWMGYPV
                     VLEMHADNSGRLGVWWMRQFWKQKTPKVMTVTTSALRKALERSTNFQFPNEALLLAPN
                     GVELEKYAGLPNPVEARRQLNLKEGLTVGFTGHIYPGRGADLLFELAKQMSAVNFLWV
                     GGTPELVDFWRGKLTEAGMTNVTMTGFVKHESIPLYQAAADVLLMPYSRSIEASSGQD
                     IAEVINPMKMFEYMAAGRGIVCADLGVIREVLNEGNAVFCEPGDVGKWKVEIEKLLAD
                     EPRRLALGAQARLDVEQLTWVKREERVIEKLRF"
BASE COUNT          990 a          736 c          595 g          839 t
ORIGIN      
        1 agcatgtcgt tcatatcaac atcttcctca aacacatgag cgactttgaa gaaatgaacc
       61 gcgctcacgt cagcaaaatg ggcgaccatc gccccgcgcg gacggtcatc ggtgtgaacg
      121 aacttcccaa acctggagta ttgctcacaa tgaatttgac ggcggtgacg aaggaataag
      181 gctataattt agaaagtaaa aagaaagccc tttcacccac aacgagggag tagcaaaatg
      241 cgcattgaaa gaattactct tactgattac cctccaatca agaatattga cattcaaatt
      301 tcagcaaacc tcgtaattgt tgctggtgca aatggttctg gaaaaactcg cctaaaagaa
      361 gctattattt atccatttgc aactgcccac gaatctcaga tttcgttatt attacgaagc
      421 actactcctg atgaagaaag cattttaggt caagagtatc tagaaatcaa tcaagggaaa
      481 atctcgacca cacttcgaaa ttatttacaa actcgcactg gaaaaaacgc cttttctggt
      541 tctattattc agatcgaatc taacagaacc gtacgtccag tccaatttca aagcattact
      601 tcaagcacgc cagatcctga cgagcaaggt ttagatcatt ctttatattt agataacctt
      661 tccaatcgtt ggaataggtt agtaaataat ctttacgcaa aatcagcaat acgaaacgat
      721 caactcgcaa gatttataaa agaaaaccct gacaaaaccg gttccgatgc cgctaaagcc
      781 attccagacc ctttagaacc ttttcaaaaa acattcagca acctattccc tgacaaaaag
      841 cttgaaccga tagatataag attcctcaga gaatttgaat atagagacaa aaattctcaa
      901 ccactttctt ttggtactct tagttcaggg gagcgggaat tagttacaat ggtttttgac
      961 ttgatatata aagaaatttc ccactctata attattattg acgaaccaga attgcaccta
     1021 catccagcat tgacctttcg atttattgaa actctcaaag gactatgcaa tggaacgaat
     1081 caaataattc tattcactca ctcggcagac ttaatatcta cttactatac taatggaaat
     1141 gtgtttgtac ttgacccaag ttctacaaat gagaatcaag ttcacatttt aaatgcgtta
     1201 aatgaaagcc attcaacaat tgctaatgcc ttaagtgaaa acctcggaat atttgctgtt
     1261 ggaaaaaata tagttttcgt agaaggggag aactcaagca ttgaccgcct tatttacggc
     1321 aaagttgcac aaagcgggtt ttcgtacttt caaattgttc ctattggttc tgttgaaaat
     1381 attatcactt taagatctgt cattaatgaa ctaagaagta cgatttttgg tgtaaggtta
     1441 tttctcataa gagaccgcga tggtttatca gataaaacaa tttcgacact agaagaaaat
     1501 tcttgttttc gatgccttcc cagacggcat attgaaaact acttactaga tgctgagata
     1561 ttaagggatg ttgcaataag tattggcatg gatgctgaaa agacaagtgc aacaaatatt
     1621 gaacgcatat tacaaaacat tgcctctcaa agcttaatgt caggagtcct atggaatgtt
     1681 cgtgaataca ttcgcttaca aggctcctta tcatcgccga agataaataa aggagaaatc
     1741 aatcaaattc caccagaaaa attagcagag acaattacac tacaactaac tagctctttt
     1801 gaagaattgt cagataaatt caactctgaa gctatattaa aattaacgct tgaagaatat
     1861 aataaactac aagaatcatt gtcatcaaac gaatggaaaa aatatttacc tgggaaaact
     1921 atttttaaca ggttttgtag tgactatttc cacgaaaagc ctgaaagaat tcaagaaatt
     1981 tacaccaact tagcactcca gaaaaaacca caagtgtttc aagatatttc taaaatcttt
     2041 cagtcattct cagaattaga atcttaattt ctctatcacc cgctcttcgc gcttgaccca
     2101 tgtcaactgc tctacatcca aacgggcttg cgcacccaac gccaatcggc gcggctcatc
     2161 agcaagcaac ttctcgatct ctactttcca ctttccaaca tctcccggct cacaaaaaac
     2221 cgcattgccc tcattcaaca cctcgcggat cacacccaaa tccgcgcaca cgattccccg
     2281 ccccgccgcc atgtactcga acatcttcat tgggttgatg acctcagcaa tgtcctgtcc
     2341 gctcgatgct tcgatggaac gcgagtacgg catcaacaac acatccgccg cagcttgata
     2401 taacggaatc gactcatgtt tcacgaagcc agtcatcgtc acgttggtca tgcctgcttc
     2461 tgtcagctta ccacgccaaa aatccaccaa ctcaggcgtg ccgcccaccc acaaaaaatt
     2521 caccgcagac atttgtttgg caagttcaaa caacaaatcc gcgcctctgc cgggatagat
     2581 atgacctgtg aatccaaccg tcagcccctc tttcaaattt aattggcgac gcgcttcgac
     2641 tggatttggc aatcccgcat acttctccaa ctcgacgcca ttcggagcga gcagcagcgc
     2701 ttcattcggg aactggaagt tggtcgagcg ctccaaagct tttctcaacg ctgatgtggt
     2761 gaccgtcatc accttgggcg tcttttgttt ccagaactgt ctcatccacc acacaccgag
     2821 ccgaccggaa ttatccgcat gcatttcgag cacaacggga tagcccatcc acgcgcccaa
     2881 caccgcggat tggggcagcc aggtgtagat caagtccgcg ccaaatttct tggcagccca
     2941 ctgggcatgg acgataaagt cgaggcgttt gagtctgctg atggatggga gcaattcaag
     3001 ttcgggagcg aggcgtaatc cgtaatgggt aagtagtgat tggtgattag taattggaga
     3061 ttctttgggg gcgaacatct tgatgtcatg tcccaattga atcaaagcct gagcgacctt
     3121 catcgcttga atcgagttgg cagttagaga tgggatgcgt
//
LOCUS       DAOQJE010000374         3154 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_20320_length_3154_cov_5.092933, whole genome shotgun sequence.
ACCESSION   DAOQJE010000374 DAOQJE010000000
VERSION     DAOQJE010000374.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3154)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3154)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3154
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20320_length_3154_cov_5.092933"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1669)
                     /locus_tag="PLT08_10340"
     CDS             complement(<1..1669)
                     /locus_tag="PLT08_10340"
                     /inference="COORDINATES: protein motif:HMM:NF024583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HNE04906.1"
                     /translation="MLNTLRHIFAPPIFEDEEKTRVASLLNSILWAVVVIGTIYTIIA
                     PIILGQFFSAILTGTIVVISFIARIFMTRGYVRAASVILLIVFDVILALSIAISGGLV
                     GASYFSLVLTTVIAGILLHGRGAYVVAGVNTLIGLSILLFQDSLPEPMIPQSPVTFFS
                     SLIVYVFFIAALLQAYARGFDKLLESLRSTQLELTSKNEEMQRFALGLENTIASRTAE
                     LDKANNRNERRAKQFEAISKIARVISQTQNLETLLPQITELISQQFGYYHVGVFLLDS
                     NSEYAVLIAANSSGGQRMLARNHKLRVGQTGIVGYVAGTMLPRIALDTGSDAIYFNNP
                     DLPDTRSEMALPLLRKGNEIVGVLDVQSTEANAFSQEDVRTLSTLADQVSVAIENSQL
                     FEEQQRILQETQAIYSRDLREGWVRFTRSQSIAGIQRRNMKNNFLPEPTDLPGTIEAI
                     RSGDVYRRTESDGSSTLTIPIKLRGQTVGVLNVKNTTNRSWTDDEVDIMAAISERAAL
                     SIENARLLEESRLIAQREHVIGEISAKIGSGTQVADILRTAVRELGSH"
     gene            complement(1683..>3154)
                     /locus_tag="PLT08_10345"
     CDS             complement(1683..>3154)
                     /locus_tag="PLT08_10345"
                     /inference="COORDINATES: protein motif:HMM:NF024583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HNE04907.1"
                     /translation="GKSGFIEATDPRGEPMLVAFAPIGLDTGWSLALAYPKSELTNTT
                     AQLRNTLIGYTIIVLTIFSVLLFIFTRSITEPLRKLTAFASRVSGKAMSSNELNFPEQ
                     IHIKTQDELEDLGNAFNQMSEELRTSFGMLEENVESRTRDMARLANELRTIAEVNSEI
                     AVIRDQNTLLNVSANLIRERLGFYHVGIFLIDERSEYAALRGASSNAAEKMLASNFRI
                     KVGEVGAVGTVTITGQAYIAQDADIDALQTSNQYLPETRSEISLPLRSYNVTIGVLDI
                     QSKVQKAFDGQDIQSLQVLADQLAAAIENAQLVQRLEQALSELSKNNLLKTQEIWHSA
                     ARRLSNSAYEYDGLQIRPIPQNISPELVSELESGHPIINNDKGKNTLFVPLTVLGQVI
                     GVIGLEQEDPSKQWSNEQIAIAQAAANRAALTLENARLFEESNRRAIKESTIFAATNR
                     VGSAVSMENILQTAAEELEKVLNDSEITIQFIDSTSEKK"
BASE COUNT          881 a          677 c          675 g          921 t
ORIGIN      
        1 tatggcttcc taattcgcgg acagcggttc gcaaaatatc tgcaacctga gtcccagacc
       61 cgatcttggc tgagatttct ccaatcacgt gctcacgttg agcgatcaaa cgactttctt
      121 ccaagagtct tgcattttca atggacaaag ccgcacgttc tgaaatggca gccataatat
      181 ccacttcatc gtctgtccaa gagcgattcg ttgtgttttt gacatttaat acgcctacag
      241 tttgtccgcg taacttaatg ggaatggtca gtgtagaaga cccatctgat tcagtacggc
      301 gataaacatc gcctgagcgg atggcttcta tagtacctgg taagtctgtt ggttcaggca
      361 agaagttgtt tttcatattg cgtctttgaa tgcctgctat gctttgtgag cgagtgaaac
      421 gaacccagcc ctctcgtaag tcacggctat aaatagcttg agtttcttga aggattcttt
      481 gttgctcttc aaatagctga gagttctcaa tagctacaga aacttggtct gctaatgtgg
      541 aaagagttcg tacatcttct tgagaaaaag cattggcttc tgtactttgt acatccaata
      601 cgccaacaat ttcatttcct ttacgcaata agggaagtgc catttcagaa cgggtatctg
      661 gtaagtctgg attgttgaag tagatggcgt cagacccagt atccaaagcg atacgaggta
      721 acatggttcc agcaacataa cccacaatac cggtttgacc gactcttaat ttgtggttgc
      781 gagcaagcat tctttgacca cccgagctgt tagctgcaat caacacagca tactcagagt
      841 tggaatctaa aagaaataca ccaacatggt aatagccaaa ttgctgactg atcaattctg
      901 tgatctgtgg cagaagagtt tccaaatttt gtgtctgact aattactcgc gcaatttttg
      961 aaatggcttc aaattgtttt gcgcggcgtt cattgcggtt gtttgccttg tctaattcag
     1021 cagtgcggga tgcgatggtg ttttccagtc ctagagcgaa gcgttgcatt tcctcatttt
     1081 tcgatgtcaa ctctaattga gtgctgcgaa gactctcgag taatttatca aaaccgcggg
     1141 catacgcctg aagcaatgcc gcaataaaga atacgtacac gatcaaggac gagaaaaaag
     1201 ttactggact ttgcggaatc atgggctctg gtaaagagtc ttgaaagagc aaaatactaa
     1261 gtccaatcaa ggtgttcaca ccagcaacga cataagcacc acgcccatgc aacaggatgc
     1321 ctgcgatgac cgtggttaat acaaggctga aatagcttgc tcctaccaac ccgccagaaa
     1381 tggcaatcga taaggcaaga attacatcaa atacgattag aagaataacc gaggctgccc
     1441 tgacatagcc acgtgtcatg aatatacgtg caataaagct aatgacaaca attgtgccgg
     1501 taagaatggc agagaagaat tgccccaaga taattggggc tatgattgtg taaatcgtac
     1561 caatcacaac cactgcccaa agaatactat ttaacaacga ggcaacacgc gtcttttctt
     1621 catcctcaaa aatgggcggt gcaaatatat ggcgcagggt gtttaacatg attttcctcg
     1681 tgttacttct tctcagaggt gctatcaata aattgaatag taatttcgga gtcgtttaat
     1741 actttttcaa gctcttcagc agcagtttga aggatatttt ccatacttac ggcagaacca
     1801 acccggttgg tagctgcaaa aatggtgctt tccttgatgg cacgacgatt actttcttca
     1861 aaaaggcggg cattttcaag tgtaagcgca gcacgattgg ctgcagcttg tgcaatagcg
     1921 atttgttcat ttgaccattg tttagatgga tcttcctgct ccaaaccaat gactccaatg
     1981 acttgtccaa gcactgtcaa tggaacaaat agagtgtttt tccctttatc gttattgata
     2041 ataggatgtc cgctttctaa ttcagaaacc aactcgggcg agatattttg tggaatgggg
     2101 cgaatttgca agccatcgta ttcgtatgca gagttactta accgtctagc cgccgagtgc
     2161 caaatttctt gagtttttaa taggttgttt tttgaaagtt cagaaagcgc ctgttcaagg
     2221 cgttgaacca actgcgcatt ttcaatggca gcagcaagtt gatcggcaag cacttgtaaa
     2281 ctttgaatgt cttgtccatc aaatgctttc tgtacttttg attgaatatc cagcacgcca
     2341 atggttacat tgtagctgcg taatggaagc gagatttcag agcgtgtttc tggtaaatat
     2401 tgattactag tttgtaaggc atctatgtct gcatcttgtg caatatacgc ttgtcctgta
     2461 atcgtaactg ttcctacggc tcctacttcg ccaactttaa ttctgaaatt acttgcaagc
     2521 attttttctg ctgcattgct acttgcacca cgtaaagcgg catattcgct tctttcatca
     2581 ataagaaaga taccaacatg ataaaagccc agacgttctc gaattaagtt cgcagacacg
     2641 ttgagaagcg tattttggtc acgaattacc gcaatttcgc tgttaacttc tgcaatggtg
     2701 cgaagctcat ttgctaagcg tgccatgtct cttgtacgtg attcaacatt ctcttccaac
     2761 ataccaaacg atgtacgcaa ctcttcggac atttggttaa atgcatttcc aaggtcttcc
     2821 aactcatcct gtgtcttaat atggatttgt tctgggaaat tcagctcatt gctgctcata
     2881 gctttgccag atacacgact tgcaaaagca gtcaacttgc gaagtggttc tgtaatagat
     2941 ctcgtgaaaa tgaatagcaa aacgctgaaa attgttaata caataatcgt atacccaatc
     3001 aaggtgttac gtagttgagc tgtggtattt gttaactctg acttaggata tgcaagggct
     3061 aaggaccaac cagtgtctaa accaatcgga gcaaaggcga ctaacatcgg ttcaccgcgc
     3121 gggtcagttg cttcaataaa accgcttttg cctg
//
LOCUS       DAOQJE010000375         3152 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_20341_length_3152_cov_5.477559, whole genome shotgun sequence.
ACCESSION   DAOQJE010000375 DAOQJE010000000
VERSION     DAOQJE010000375.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3152)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3152)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3152
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20341_length_3152_cov_5.477559"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..569
                     /locus_tag="PLT08_10350"
     CDS             <1..569
                     /locus_tag="PLT08_10350"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: protein motif:HMM:TIGR00589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HNE04908.1"
                     /translation="RLHDLFVNTEAVTPGEYKSRGAGIDIHYGIHLTPFGKCLIATTD
                     RGICNLSFVNASEGKAIDDLVAHWSHANMIEDYQATAPLVNRIFLGAQLDSPLKLHLR
                     GTNFQIKVWEALLSIPTGALTTYEHIATTIGNPRAVRAVGSAVGDNPIAYLIPCHRVI
                     RKSGEFGNYLYGSARKKAILAREFVMTE"
     gene            594..1526
                     /locus_tag="PLT08_10355"
     CDS             594..1526
                     /locus_tag="PLT08_10355"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HNE04909.1"
                     /translation="MKTKLWTALIALWIVWGSTYLAIRFAVETIPPFLHASLRFLVSG
                     AILVVWRHMAGDPKPTTSNWKATAIVGTFLLLGGNGFVSLAEQSVPSGIAALMITTSP
                     FWLVLFESLRSGGTKPSWQAIVGLLLGFGGVFVLVGPGEIAGSQHTFETSGVILLVLA
                     PILWSMGSIYSRGADMPKSTLMSTGMQMLAGAGSLFIVSVATGELSGFNFGDVSMRSW
                     WGLIYLITFGSLVGFVSYGWLIQNAPVSLTSTYAYVNPMVAVLLGWLFAGEELNARIA
                     VSSVIIIGAVIMINLARNSKPAPKNEMAIPEEIE"
     gene            1593..2936
                     /gene="glnA"
                     /locus_tag="PLT08_10360"
     CDS             1593..2936
                     /gene="glnA"
                     /locus_tag="PLT08_10360"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008090340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamate-ammonia ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glutamate--ammonia ligase"
                     /protein_id="HNE04910.1"
                     /translation="MDSKELLKRVKEDNVKFLSLQFTDVLGAVKSVDMPIRHLEDVLK
                     DGAWFDGSSVEGFARIQESDMRLKVDPETYVLLPWSSPESRRARVFCDIYTPSGEPFE
                     GDPRGALKRILKKIDERGWKFNIGPEPEFFLFKGNDGSGNVHPVPHDTGGYFDFSSFD
                     EAVVVRTALMDALDQMGLDVEVGHHEVALGQHEIDFRFADALKAADNVLTLKYTVKAI
                     AAQHGLVASFMPKPVYGINGSGMHCHQSLFDTKTGNNLFFDAKDEAHLSQLAYSFIAG
                     QLEHARALAGVVAPTVNSYKRLVPGYEAPVYIGWAQQNRSALIRIPRYTEGRDKAVRA
                     ELRFPDPSSNPYLAFTVMLAAALDGIDKNMKAPKPLNNINLYHLNKEERAKLGVTELP
                     GSLAESLKELEKNEVLKAALGDYLFEAYMRAKWEEWDEFRLRVTDFEIQRYLETA"
BASE COUNT          769 a          764 c          769 g          850 t
ORIGIN      
        1 ggcggctcca tgatcttttc gtcaacactg aggcggtgac tcccggcgaa tacaaatcgc
       61 gcggggcagg catcgatatt cactacggta tccacctcac gccgtttggc aaatgtctca
      121 tcgccactac cgaccgtggc atttgtaatc tcagctttgt aaacgccagt gaaggcaaag
      181 caatagacga tctagtggca cactggagcc atgccaatat gatcgaagat tatcaagcta
      241 cggcgccatt ggttaaccgc atctttcttg gggcgcagct tgattcacct ctcaagctgc
      301 atctacgcgg cacaaatttc cagatcaaag tgtgggaagc cctgctcagc attcccaccg
      361 gtgcattgac cacctacgaa cacatcgcta cgacaatcgg aaatccgcgt gcggttcggg
      421 cagtgggcag tgctgttggt gataacccta ttgcctatct catcccctgc catcgggtga
      481 ttcgcaagag cggcgagttt ggcaattatc tgtatgggtc ggctcgcaaa aaagccatct
      541 tggcaagaga atttgtaatg acagagtaaa ttaattaaac aaaaaggaaa agaatgaaaa
      601 caaaactttg gaccgcatta atcgctttat ggattgtttg gggctctacc tatttagcta
      661 ttcgttttgc cgtagaaacc attccacctt tcttgcatgc atctctgcgc tttttggttt
      721 ccggtgcgat tctggtcgtg tggcgtcata tggctggcga ccccaaaccc accacaagca
      781 actggaaagc caccgccatt gtcggtacct tcctcctctt gggcggaaat ggattcgtaa
      841 gccttgccga acaaagcgtc ccttcaggga ttgcagcact catgatcacc acttccccat
      901 tttggttggt gctttttgaa tcactgcgct ctggtggcac aaaaccttcc tggcaggcaa
      961 ttgtgggtct attacttggc ttcggcggtg tgtttgtatt ggtgggtccg ggagaaattg
     1021 caggctcaca acatacgttt gaaacaagtg gcgtaattct gcttgtattg gcgcctatct
     1081 tgtggtcgat ggggtcaatc tattcacgcg gtgccgatat gccaaaatcc acgctcatgt
     1141 ctacgggtat gcaaatgctg gcaggtgcag ggtcgctatt catcgtcagc gtagcaaccg
     1201 gcgaattaag tggattcaac ttcggcgatg tgtcgatgcg ctcttggtgg ggtctgatct
     1261 acctaatcac ctttggttcc ttagttgggt tcgtttccta cggttggtta atccaaaacg
     1321 cacctgtgtc attgacctcg acttatgcgt acgtcaaccc gatggtggcg gtgctccttg
     1381 gttggctctt cgcaggtgaa gagttgaatg cgcgtattgc cgtctcatca gtaattatta
     1441 tcggtgcggt catcatgatc aaccttgcgc gaaattccaa gcctgctccc aaaaatgaaa
     1501 tggcaattcc agaagaaatt gaataagaag tacacttgaa gctgttggtc agtatcaaaa
     1561 actgtgtaag caaaatcaaa aaggagatga acatggattc aaaagagttg ctcaagcggg
     1621 tgaaagaaga caatgtcaaa tttctttcgc tccaatttac agatgtgctc ggggcggtca
     1681 aaagtgtgga tatgcccatt cgccacctcg aagatgtgtt gaaagatggt gcttggttcg
     1741 acggttcttc tgtagaaggc tttgctcgta ttcaagaaag cgacatgcga ctcaaagtag
     1801 accctgaaac ctatgtgttg ctgccatggt cttcccctga gtctcgccgc gcgcgtgtgt
     1861 tctgcgatat ctatactcca tcaggtgaac catttgaagg cgacccacgc ggcgcattga
     1921 agcgtattct caaaaagatt gatgaacgcg gttggaagtt taatattggt cctgagccag
     1981 agttcttcct gttcaagggc aacgatggca gcggtaatgt tcaccccgtc ccccacgata
     2041 ctggtggtta ttttgatttc tcttcatttg atgaagccgt tgttgtgcgc accgctttga
     2101 tggatgctct cgatcaaatg ggactcgatg tggaagttgg gcatcacgaa gtggcgttgg
     2161 gtcagcatga aattgacttc cgcttcgcag acgcgctcaa agctgcagat aacgtcctga
     2221 cgcttaagta cacggtgaag gcgatcgccg cccaacacgg actcgtcgct tcgttcatgc
     2281 ccaagcctgt ttatggcatc aacggttctg gtatgcactg ccatcaatca ttgttcgaca
     2341 ctaagaccgg caataatttg ttcttcgatg ccaaggatga agctcatctc tcgcagttag
     2401 cgtattcgtt catcgccggt caactcgaac atgcccgcgc ccttgctggc gtcgtcgctc
     2461 cgaccgtgaa ctcatacaag cgtttagttc cgggttatga agcgcctgtg tacatcggtt
     2521 gggctcagca gaaccgctcg gcgctcatcc gtattccgcg ctacacagaa ggtcgcgata
     2581 aagccgttcg cgcggaattg cgcttccctg atccttcgtc caacccgtat ttggcgttca
     2641 ccgtcatgct tgccgctgcg ctcgatggta tcgacaagaa catgaaggcg cctaaaccac
     2701 taaacaacat caatctctat cacctgaaca aagaagaacg cgccaagctc ggtgtgaccg
     2761 aactccctgg ctcgttggct gaatctttga aggaattaga gaagaacgaa gtcttgaagg
     2821 cagctcttgg agactatctg tttgaagcct atatgcgtgc caagtgggag gaatgggatg
     2881 aattccgcct acgcgtgaca gattttgaaa ttcagcgcta tttagaaaca gcataaacgt
     2941 tctgactagc caaaacaaaa ctgcactcag aaaattctga gtgcagtttt gttttattcc
     3001 ttgaaattca ctttgtctta taattatttt caatgaaaaa gatttttgct tttgctatct
     3061 taagtgttct attatgcagt gcatgttctc aaaataaagc atatccgctt aaattggcaa
     3121 ctttcgacaa aaattcggta agcgtcttaa tc
//
LOCUS       DAOQJE010000376         3150 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_20367_length_3150_cov_5.625525, whole genome shotgun sequence.
ACCESSION   DAOQJE010000376 DAOQJE010000000
VERSION     DAOQJE010000376.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3150)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3150)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3150
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20367_length_3150_cov_5.625525"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..686
                     /locus_tag="PLT08_10365"
     CDS             <1..686
                     /locus_tag="PLT08_10365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE04911.1"
                     /translation="GLFRLQQVDRQIDRAKARLDAIQKTLENDKELKTAQTRLEKNSA
                     ENQQALKTLKNIELEAETLRIKIEQSNSSLYGGTVKNPKELQDLQKEIESLKKHQETL
                     EERQLEAMSQSENTEAEVEKAKAELEVIRSRLGNEHKKLLDEQAELSKQMASFGQERE
                     AALSPIDVSLLTSYEDLRKQKRGVAVTEVEDGACASCGTTINAALQQSARSQKLSYCP
                     SCGRILFAN"
     gene            696..>3150
                     /locus_tag="PLT08_10370"
     CDS             696..>3150
                     /locus_tag="PLT08_10370"
                     /inference="COORDINATES: protein motif:HMM:NF017539.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HNE04912.1"
                     /translation="MFLLNIIDPLSFIAGFATATVIWFLIGRARPMLAELREGLKEQR
                     EQAQTRKASSVEENHRRLTLRRAQGMHLAAQLFALDEILQEPTVITPPAPVEPGGAAR
                     FEDVVTQTLPYMPAWPEVAAIYQPHTLTLPQALAGDTNLVIIGQPGTGKTVALAHLAS
                     LAANRSEKLGALQSFVPFHFHVADLNLPVQDPKNILNVISDAAAENAPMFDVGKIPAF
                     VQSVFRSGEVLLLVDGYDEITPDEQTLVAEFFKLLLKAHPKTRIVTTGAPEYLDGLIP
                     LGFVPLSLCTWTEKGNQEFVDQWGELWSRTVAMEAWAQTGPEQVDPLLINSWLGSASL
                     NLSPLELTLKIWGAYAGDSLGPNILDTIATHIRRIAPANTPLAALETLAMQVVVSATP
                     VFDPRKAREWVKSFEVAEETPEGETAQSPEGEAQPSEDAKSKKGKKVEKVATPNTGLL
                     GKMTESGLLVSYPNNRMRFIHSVLAGYLAGHALTNYNAEETLLNQPDWSGKYLTMRFI
                     AAHGDASRLVQAMLEFSRLPMHRPLFAAARWLRDAPKNAPWRGKMFGTLAAILQTEGI
                     PLSLRGQAMAAFVYSNDASAATLFRQFATSSSFELIHLAILGLGAVRDTKSLKILEGA
                     LNAPSLAVKRAACMALAAINTNESLEIAAHALLNGEEDVRRAAGEALANDPKEGHAML
                     RDGITINDILLRRAVIYGLGRVREEWATDLLKKVQIEDEQWVVRNAATEVLDMQAQSY
                     AYAPMKPKAPHETPWLIEFAAKKGVGVSPGVPATDILLVALKDENPETRLAAIQFLKY
                     TPQEIVLKQLYEAMYKDDPE"
BASE COUNT          865 a          802 c          739 g          744 t
ORIGIN      
        1 tgggactttt ccgccttcaa caagtagacc gtcagattga ccgcgccaaa gcacgtttgg
       61 atgccattca aaagaccttg gaaaacgaca aagaactaaa aacggcgcaa acccgtttag
      121 aaaaaaacag tgctgaaaac cagcaagccc tgaaaacctt gaaaaatatc gaacttgaag
      181 ccgagaccct tagaattaag atcgagcaat cgaattcaag cctttatggt ggcacagtaa
      241 aaaacccgaa agaacttcaa gacctgcaaa aagaaattga gtctcttaaa aagcaccaag
      301 agactttaga agaacgtcaa ctagaagcca tgtcgcaatc agagaatacc gaggctgaag
      361 tagaaaaagc aaaagccgaa ttggaagtga ttcgctcgcg gcttggcaat gaacacaaaa
      421 aattgctgga tgaacaagcc gaactgagta aacaaatggc aagttttggt caggagcgtg
      481 aagcagccct ttctcctatt gatgtttctt tacttacctc ttacgaagac cttcgcaaac
      541 aaaaacgcgg agttgcagta actgaagtag aagatggtgc ttgtgcatct tgtggcacga
      601 ccatcaacgc agctcttcaa caaagtgctc gttctcaaaa actttcatat tgcccttctt
      661 gtgggcggat cctcttcgca aactaggcga gtgacatgtt cttattgaat atcattgacc
      721 ctctttcatt catagcaggt tttgctaccg ccaccgtcat ttggttctta attgggcgtg
      781 ctcgccccat gctcgctgaa ttgcgtgaag ggttgaaaga acaacgcgag caagcccaaa
      841 cccgcaaagc aagttcggtt gaagaaaatc accgccgcct tactttacgc cgtgcacagg
      901 ggatgcatct tgccgcacaa ctctttgcat tggatgaaat cttacaagaa cccaccgtca
      961 tcacaccgcc tgcaccagta gaacccggcg gcgcagcccg ctttgaagat gtggtcaccc
     1021 aaaccctgcc ctacatgcca gcctggcccg aagtggcagc gatctaccaa ccccatacac
     1081 ttactctgcc acaagccttg gctggggata caaacctcgt cattatcggt cagcccggca
     1141 ccggtaaaac tgtagctctc gctcatttag caagccttgc cgcaaaccgc agtgaaaaat
     1201 taggtgcact gcaaagcttc gttccgtttc actttcatgt agcagactta aatctgcccg
     1261 ttcaagaccc caagaatata ctgaatgtca ttagtgacgc tgccgccgaa aatgcgccca
     1321 tgttcgatgt cggcaaaatt ccggcatttg ttcaatccgt ctttcgaagc ggagaagtgc
     1381 ttttactcgt tgatggctac gacgagatta cacccgatga gcaaaccttg gtggcagagt
     1441 tcttcaaact attactcaaa gctcatccaa agactcgcat cgtcacaact ggtgcgccag
     1501 aatatttgga tggtttaatt ccgcttggct ttgtgccgct aagtctgtgt acatggacag
     1561 aaaaaggcaa tcaggaattt gtggatcaat ggggcgagtt atggtctcgc accgttgcaa
     1621 tggaagcttg ggcacaaacg ggacccgagc aagtggaccc gcttcttatc aactcctggc
     1681 ttggttctgc gtctctaaat ttatctcccc ttgagttgac tctcaaaatt tggggcgcat
     1741 atgcaggcga tagtttaggt cctaacattc tcgataccat cgccacccat attcgacgca
     1801 tcgccccagc caacacaccg cttgccgcgc ttgagacgct tgccatgcaa gttgtcgtca
     1861 gtgccacgcc cgtatttgac ccgcgcaaag cgcgtgaatg ggtgaagtcg tttgaagtgg
     1921 cagaagaaac tcctgaaggc gaaacagctc aaagtccaga aggtgaggcg cagccatcag
     1981 aagatgccaa gtctaaaaaa ggcaagaagg tcgaaaaagt tgctacgccc aatacaggtc
     2041 tgctcgggaa gatgaccgaa agcggattgt tagtgtcata tcccaataac cgtatgcggt
     2101 ttattcattc ggtattggct ggatatcttg cgggtcatgc attgaccaat tacaacgccg
     2161 aagaaacatt gttgaatcaa cccgattggt ctggaaaata tctcaccatg cggtttattg
     2221 ccgcacatgg tgatgccagc cgattggtac aagccatgct cgaattttca cgcctaccca
     2281 tgcaccgacc gctatttgcg gcggctcgct ggctacgtga tgcgcctaaa aatgctccat
     2341 ggcgcggaaa aatgtttgga accctcgccg caatcttgca aacagagggc atccccctca
     2401 gcctgcgcgg acaagccatg gcagcctttg tgtatagcaa tgatgccagc gctgcaactt
     2461 tattccgcca gtttgccaca tcaagttcat tcgaattgat tcatctcgcc attcttgggc
     2521 ttggcgccgt acgcgatacg aaatccttga agattcttga aggcgcattg aatgctccga
     2581 gccttgctgt taaacgtgca gcttgtatgg cattggcagc catcaacacc aatgagtcgc
     2641 ttgaaattgc cgcacatgct ttgttgaatg gcgaagaaga tgtgcgccgt gccgcaggtg
     2701 aagcccttgc caatgatccc aaagaaggtc acgccatgtt gcgcgatggt attaccatca
     2761 acgatattct cctgcgccgt gctgtgattt atggtcttgg gcgtgtccgc gaggaatggg
     2821 cgactgacct gcttaagaaa gttcaaattg aagacgaaca atgggttgtt cgtaatgccg
     2881 ctacagaagt gttggatatg caggcacaaa gctatgccta tgcacccatg aagcctaaag
     2941 ctccgcatga aaccccatgg cttattgagt ttgccgccaa gaaaggcgtg ggcgtttcac
     3001 ccggtgtccc tgccacagat attttgttgg ttgccctgaa agatgaaaac cctgaaactc
     3061 gcttggcagc cattcaattc ttaaagtaca caccgcaaga gattgtgctc aaacaattgt
     3121 atgaagccat gtacaaagat gacccagaat
//
LOCUS       DAOQJE010000377         3145 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_20410_length_3145_cov_5.907767, whole genome shotgun sequence.
ACCESSION   DAOQJE010000377 DAOQJE010000000
VERSION     DAOQJE010000377.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3145)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3145)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3145
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20410_length_3145_cov_5.907767"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(65..1393)
                     /locus_tag="PLT08_10375"
     CDS             complement(65..1393)
                     /locus_tag="PLT08_10375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE04913.1"
                     /translation="MSSEALAKPTITKNSSEFKQDTYASLSTGELVVFAVQVLQADAA
                     PVAVEEVVSTCFRLFPHSFALKNYFYWPDSALVARRLHDAKEKGLLKGNSTDGYEAKS
                     SGKQVAKQVAKALGVSLPVPPKVEVVAPPVVVEAPKAAEIVAEKKEEEKKESVPSAPV
                     ARKVKKQAQKKVEKKAPAKKPVAKTTPKKDATTSKKKAQPKATAKKPVKPAPKPKATV
                     VKKDEKGKKKSKPAAKPDVKKAALKVEKKVKPTVKPEVKKVEPKVVEKKKETRQEKAK
                     PPVKPEIKKAAPKVEKKEAKSQPTQLSMPLAPATAWKKAEAPASKPKVQKEAPSSGDR
                     LPVKGEVLAPVAVSKEEKDKAAKVIKQIERSDAYQLYRRNGKRAQIGEFDFRNMLFAT
                     MESSAETLKRNLDLFKRYASIHFRTDLIAFLEFCEENFAALMISKVKGKK"
     gene            1554..2339
                     /locus_tag="PLT08_10380"
     CDS             1554..2339
                     /locus_tag="PLT08_10380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE04914.1"
                     /translation="MYTYPIKFEFPMISIGRQVDVKNGDGKFIMRASDPFISFKDAIS
                     YVDGGGKEVYRAEGDSSFRFMFQFASMSTEWNVVTADGKPVAYVDDYWARMEEFKDVN
                     VNITSSAPVMQSDGRASLGGVLGNVAANLANNAVDYAVRSNVPSRLVYQVKTHAEGNV
                     LGWIVPSRGTGWFDFLPYSTRIQILNLPFAARAYAPSYEFKIGNLYGPTVLKLQKQRD
                     LLVDKYTLEKAGELTDQQERWAIPALTLVAMFERTRVKQMADW"
     gene            complement(2398..>3145)
                     /locus_tag="PLT08_10385"
     CDS             complement(2398..>3145)
                     /locus_tag="PLT08_10385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE04915.1"
                     /translation="QVWSNASGYLNQVLAGSLVPIFGTWLDTMFSWLFILLNIAILFS
                     LVLGMFTTKFKFEWIHIYFVIYTAGILAFWNPQVGSVKARFLIPVLPFLYFYFLSGLK
                     SLTRENPRLVIGVACGIALVLLARNLQDWRSPIREQMTDLSIGTSWVSENAPADAIVM
                     VNEPVPAYVHVKHKTINFPKNDQDLEQYIKKQDIEYIIIAPLLQSPKSTELSKDIQAI
                     QSQIESQPNAFQVVFESKENNVTVYQYTGQ"
BASE COUNT          725 a          712 c          711 g          997 t
ORIGIN      
        1 tggtcagcct gctggatcgc aacgcggtct gcgtactgta attttctact actgcctgcc
       61 ataactattt tttaccttta actttcgaga tcattaaagc tgcaaaattt tcttcacaaa
      121 attcgaggaa cgcgatcaaa tccgtacgga agtgaatgct ggcatatcgt ttgaacagat
      181 ccaaatttcg tttcaaggtc tctgccgaag actccatggt agcgaacaac atatttctga
      241 aatcgaactc gccaatttga gcacgtttcc cattgcgtct gtataactga tatgcatctg
      301 accgctcaat ttgtttgatg accttcgcgg ctttgtcctt ctcttctttg gatactgcca
      361 ccggagccaa cacttcgcct ttgacgggca gcctgtctcc gctagagggc gcttctttct
      421 ggacctttgg cttgcttgct ggcgcctcag cttttttcca cgctgttgcc ggagcaagcg
      481 gcatgctcaa ctgtgtaggc tgagactttg cctctttctt ctcaactttc ggtgcagcct
      541 tcttgatttc tggctttacc ggcggtttgg ctttttcttg tcttgtttct tttttcttct
      601 ctacaacctt cggctctacc tttttaactt ctggttttac tgttggcttt actttcttct
      661 caacctttag cgcagccttc ttaacgtctg gcttcgccgc aggctttgat ttcttctttc
      721 ctttttcgtc tttctttaca acagtcgctt tgggctttgg tgctggtttt accggtttct
      781 ttgctgttgc ttttggctga gctttctttt ttgaagttgt ggcgtctttc ttcggcgttg
      841 tctttgcaac aggtttcttg gcgggcgcct tcttttcaac ctttttctga gcttgcttct
      901 ttactttcct tgctactggt gcagaaggga cagattcttt cttctcttct tctttctttt
      961 ccgcaacgat ttctgcagcc ttgggtgctt caacgactac aggaggggca accacttcca
     1021 ctttgggtgg tacaggcaga gaaaccccta gagcctttgc tacctgttta gcaacttgtt
     1081 tgccagagct tttggcttca tagccgtctg ttgagttgcc tttgagaagc cctttttctt
     1141 tggcgtcgtg taaacggcgg gcaaccaatg ccgagtctgg ccagtagaag taatttttga
     1201 gagcaaaact atgggggaaa agcctaaaac atgttgagac tacttcttca acagcgaccg
     1261 gagcggcgtc cgcttgtaga acttgaacgg caaatacgac caactctccg gtagagagag
     1321 aggcgtatgt atcctgcttg aactcggatg aatttttggt gatggtcggt ttggctagcg
     1381 cctcagatga cataagtcca tgtgccttgg ttgaaagttt cgcaatttta cactggtaat
     1441 agtaccagag acatactcaa ttcgggtcaa aatccaacct atatcctatt tcatcagggg
     1501 gaataatccg tagaatggag caagttgttt atttcattca ggagaaatta ttcatgtata
     1561 cctatcctat caagttcgaa tttccgatga tcagtattgg tcgtcaggtg gacgtcaaga
     1621 atggcgatgg caagttcatt atgcgagctt cagacccgtt catttctttc aaagatgcta
     1681 tttcttatgt ggatggcggc ggcaaggaag tgtatcgtgc agaaggagat agctcttttc
     1741 gcttcatgtt ccaatttgcc agcatgtcta cagagtggaa tgttgtgact gctgatggta
     1801 aacctgttgc ctatgtagat gattactggg cacgcatgga agaattcaaa gatgttaatg
     1861 tgaatatcac ctcttcagct cctgtgatgc aaagcgatgg gcgagcttct cttggtggtg
     1921 tgcttggtaa tgttgctgcc aacttagcca acaatgcagt cgattatgca gttcgctcga
     1981 atgtcccttc tcggcttgtg tatcaggtta aaacccatgc tgaaggcaat gtgctggggt
     2041 ggatcgtccc ttcgcgtggt accggctggt ttgacttttt accctattcc acccgtattc
     2101 aaattcttaa tctgcctttc gctgctcgtg cttatgctcc ttcttatgaa ttcaagattg
     2161 gtaatttgta cggacctacg gtgttgaaac tacagaaaca acgtgacttg ctcgttgata
     2221 aatataccct tgaaaaagct ggtgaactga cagaccaaca agaacgctgg gcgatccctg
     2281 ccttgacctt ggtggcgatg ttcgagcgca ctcgcgtcaa gcagatggca gattggtaat
     2341 tagcaaatcg gtaggttggt attgaaaaaa gcttccggcg ttatcgccgg aagcttttta
     2401 ttgcccagta tattgatata ccgttacgtt gttttcttta ctttcaaaaa ctacttgaaa
     2461 ggcattcggc tggctttcga tttgggattg tatcgcttga atatctttac ttaattctgt
     2521 gctttttggc gattgcaata gcggagcaat aatgatgtac tcaatgtctt gtttttttat
     2581 atattgctcc aagtcttgat cgttctttgg aaagttaata gtcttgtgct ttacatgcac
     2641 ataggctggc acaggctcat tcaccatcac gattgcatcc gctggcgcat tttcagaaac
     2701 ccaactggtt ccaatagaaa ggtctgtcat ttgctcgcgg ataggggatc gccagtcttg
     2761 cagattgcgg gcgagcaaaa ccaatgcaat gccacaagca actccaatga caaggcgcgg
     2821 attttctctt gtgagcgatt tcaatccact taagaagtaa aaatataaaa atggcaaaac
     2881 gggaatcaag aagcgtgctt tcacacttcc cacttgcggg ttccaaaacg ccaaaatacc
     2941 agccgtgtaa atgacaaaat aaatgtgtat ccactcgaac ttgaatttgg tggtgaacat
     3001 gcccaacaca agcgaaaaca agatggcgat gtttaataga atgaacagcc aactgaacat
     3061 cgtatccagc cacgtgccga agatcggaac taatgacccg gcaagaactt gattcaaata
     3121 gccggatgca tttgaccaga cttgc
//
LOCUS       DAOQJE010000378         3145 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_20418_length_3145_cov_3.946602, whole genome shotgun sequence.
ACCESSION   DAOQJE010000378 DAOQJE010000000
VERSION     DAOQJE010000378.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3145)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3145)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3145
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20418_length_3145_cov_3.946602"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..746
                     /locus_tag="PLT08_10390"
     CDS             <1..746
                     /locus_tag="PLT08_10390"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013434.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HNE04916.1"
                     /translation="LNPAQYVTGLAGAAARAGATLCARARVNKIEWRESRFFIQTERG
                     SLEAESVLVAISGYTGGVTKKLQKKIIPIGSFIIATERLSAELAHELSPKNRMIFDYK
                     HFLNYFRLWDNRMIFGGRAAFFPENENTIARSGEILRNEMIKVYPQLKDVNVDYVWGG
                     TLDFAFDQMTHVGEEDGIFYSLGYAGHGVAMATYLGATVAEAMLKGNIKEHPFAKFDF
                     PSAPMGLYNGNPWFLPFAGMYYKVLDWVE"
     gene            complement(719..2065)
                     /locus_tag="PLT08_10395"
     CDS             complement(719..2065)
                     /locus_tag="PLT08_10395"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HNE04917.1"
                     /translation="MPSLFNRLFRKEKSTSHKIVAIKVGVVVNQRYQLESELGRGGMG
                     IIYKAHDLTENREVAFKVINPETANSLSLNQFAREADILSSFDHPHIVSVHETGFVNE
                     DPSLPFLVMELLHGRPLSEIGVLTYSRIVSIAKQICEALTYIHSQGFVYRDIKPNNIL
                     LEKRGFDYFVKLIDFGLARPLGDAYLPNESSLAGTVFYLAPELIEGQVADVRSDLYAL
                     GILLYEMIVGRVPFSDVDEATIQLQHQQQKASPPSQSRLGVPMELDTLVLRLLEKNRQ
                     DRPASANEVCEILNSIRFDKKTQGNLPSNISDRSDAQPIIQLLAEHSLVTLISNDVPL
                     ALAAVSHLSEQFPDGVWVVNLEAVSEPVAVLPMVCSMFGVKEIQNRPLAVMSIEFLRE
                     KNLLLLLTHCGHVSGACAQLASAITVSCPDVQIMAVSDKPLNLPNEKVYSTQSKTL"
     gene            2148..2879
                     /locus_tag="PLT08_10400"
     CDS             2148..2879
                     /locus_tag="PLT08_10400"
                     /inference="COORDINATES: protein motif:HMM:NF024258.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HNE04918.1"
                     /translation="MRILVVSDIHANFVAFEAVLKDAGSFDTVWCLGDVVGYGPQPNE
                     CVEQLRELHPVCLAGNHDLAVTGQIALWDFTKDAQEVIFWTRHWLTQENMEWLTTLSS
                     TPQVEHGITIVHASLRDPVWEYITNASLVKDNIQFAQTPICLHGHTHLATLFRKKWDE
                     YRILEEYPRLNTPIKLIPDQMFINPGSVGQPRDEDPRAAYALIDTDEMTYTQKRVLYD
                     VTTVQGLMKQAKFSNRLIRRLRFGQ"
     gene            2897..>3145
                     /locus_tag="PLT08_10405"
     CDS             2897..>3145
                     /locus_tag="PLT08_10405"
                     /inference="COORDINATES: protein motif:HMM:NF012791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HNE04919.1"
                     /translation="MYKKILVPLDGSAVAEGVLPHAKSLAYSEGAELILLTVAANPAM
                     DYLFSDPGIAETAVQEQVEKSKSYISTIEKELSTAGFKV"
BASE COUNT          869 a          713 c          778 g          785 t
ORIGIN      
        1 ggttgaaccc ggctcaatat gtgacggggt tagcgggtgc ggcagctagg gcgggggcaa
       61 cactttgcgc gcgagcgcgg gtgaacaaga tcgagtggag agagagccgg ttcttcatcc
      121 aaacagaaag agggagttta gaggcggagt cagtcttggt ggcgatatcg ggttatacgg
      181 gcggagtcac aaagaagctg caaaagaaaa ttatcccgat cgggtcattc attattgcaa
      241 cggaaagact ctcggctgaa cttgcgcacg aactcagccc gaagaaccga atgatctttg
      301 attacaaaca tttcttgaat tattttcgcc tgtgggataa ccgcatgatc tttggcggac
      361 gggcggcgtt cttccctgaa aatgaaaaca ctattgcgcg aagcggcgag atattgcgca
      421 atgaaatgat aaaagtctac ccgcagttga aggatgtaaa cgtggactat gtgtggggcg
      481 gcacgctcga ttttgcattc gaccaaatga cgcatgtagg cgaagaagat gggatctttt
      541 actcgctagg ctacgccggg catggtgtgg caatggcgac ctatttgggc gctacggttg
      601 ccgaagccat gctaaaggga aatatcaaag agcacccgtt tgcaaagttt gatttccctt
      661 ctgcgccaat gggattgtat aacggcaacc cgtggttttt gccgtttgcc ggaatgtatt
      721 acaaggtttt ggactgggta gaataaactt tttcgttggg taaattcaac ggcttgtcac
      781 taactgccat gatttgaaca tcggggcaag aaactgtgat ggcagatgcc agctgagcgc
      841 atgccccaga gacatgtccg caatgcgtga gaaggagaag aaggtttttc tcgcgcagaa
      901 attcgatcga catgaccgca agcggacgat tttgaatctc ttttacgcca aacattgaac
      961 agaccattgg aagaacagcg acaggttcac ttactgcttc caagttaaca acccaaacac
     1021 catctggaaa ttgttccgaa agatgtgata cagcagctaa cgcaagcggc acgtcattgc
     1081 taatgagagt cacaagtgaa tgttccgcaa gcagttggat aatgggttga gcatctgaac
     1141 ggtcagaaat attggatggc aaattccctt gagtcttttt atcaaagcga atcgaattca
     1201 agatctcaca tacttcattt gcggatgccg ggcgatcttg ccgatttttc tcaagcagac
     1261 gcaaaacaag cgtatctaat tccatcggca cgcctaagcg agactggctg ggtggagatg
     1321 ctttctgctg ctgatgttgg agttgaattg tggcttcgtc cacatccgaa aacggcacac
     1381 gcccgacgat catttcatac agcaagatac ccagcgcgta taagtcagac cgcacatcag
     1441 cgacctgacc ttcaatcaat tcgggggcga ggtaaaaaac ggttccggca aggctgcttt
     1501 cgttcggcag atatgcatcc cctagcggtc gggctagccc aaaatcgatc agctttacga
     1561 aataatcaaa tccacgtttt tcgagcagga tattattcgg tttgatgtca cgatacacaa
     1621 agccctgact atgaatatat gtcaacgctt cacatatctg ttttgcgatt gagacaatgc
     1681 gagaataggt cagtacgcca atttcagaaa ggggtcttcc gtgcaagagt tccatcacaa
     1741 gaaaaggcag tgatgggtct tcattgacaa accccgtctc atgcactgaa acaatatggg
     1801 gatgatcgaa gctagaaaga atatccgctt cacgcgcaaa ttggttcagc gaaagtgaat
     1861 ttgcagtttc ggggttgatg actttgaagg caacttcgcg gttctcagtt aaatcatgag
     1921 ccttataaat aatgcccata ccaccacgcc caagctcact ctccaattgg tagcgttggt
     1981 tgacaacaac tccaacctta atagcaacta tcttgtgcga agtggatttc tccttgcgaa
     2041 agagacggtt aaaaagagag ggcatgttca aattatagcg ttgctgtcat ctgcaaaaaa
     2101 gttactttcg tcattctttc tgtacgctat aatattttta ggtatccatg cgcattcttg
     2161 ttgtatcaga tatccatgcg aacttcgtcg cgtttgaagc ggtgttgaaa gatgccggta
     2221 gttttgatac agtctggtgc ttgggcgatg tggtgggata tggtccgcaa ccgaacgaat
     2281 gtgtagaaca actgcgagaa ttacacccag tttgtctggc gggcaatcat gaccttgccg
     2341 taacaggtca aatcgcgtta tgggatttca ctaaagacgc acaagaagtc attttttgga
     2401 cgcgtcactg gctcacacaa gaaaatatgg aatggctgac aaccttgtca tctacgcctc
     2461 aagttgagca cggcattacc atcgtccacg ccagcttgcg cgacccggtc tgggaataca
     2521 tcaccaatgc cagcttggtc aaagacaata tccaattcgc tcaaacccct atctgtttac
     2581 atggacatac tcatcttgct actctctttc ggaagaaatg ggatgaatat cgcattctgg
     2641 aggaatatcc gcgcctcaac acgcccatca agctgatccc cgaccaaatg tttatcaacc
     2701 ctgggagcgt aggtcaacca cgcgatgaag atccgcgtgc cgcttatgcc ctaatcgaca
     2761 cagatgagat gacctacact caaaagagag ttctttacga tgtgacaaca gttcaagggc
     2821 tgatgaaaca ggcaaagttt tcaaatcgcc tcattcgacg tttacgtttc gggcaataag
     2881 cccataggag gaagttatgt ataaaaagat tctggttcca ctagatggtt cggcagtcgc
     2941 cgaaggcgta ctcccccacg caaaatcatt ggcatattct gaaggcgccg agttgattct
     3001 gctaaccgtt gcagccaacc cagccatgga ctatcttttt tcagaccctg gcattgcgga
     3061 aactgcggtt caggagcaag ttgaaaaaag taagtcctat atttctacca ttgaaaaaga
     3121 gcttagcact gccgggttta aggtt
//
LOCUS       DAOQJE010000379         3136 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_20523_length_3136_cov_4.814346, whole genome shotgun sequence.
ACCESSION   DAOQJE010000379 DAOQJE010000000
VERSION     DAOQJE010000379.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3136)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3136)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3136
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20523_length_3136_cov_4.814346"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..506
                     /locus_tag="PLT08_10410"
     CDS             <1..506
                     /locus_tag="PLT08_10410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HNE04920.1"
                     /translation="GMINQVFRRLGVHWDVQGTNIYVPAEQNLVIASDLDGAIPEIKT
                     NVWPAFPTDLTSIAITVATQVSGSVMFHDWMFSGRMYFTDKLAGMGARIILCDPYRCL
                     IQGPTQLYGEKLESPDIRAGMAMLLAALAANGTSKIRNIVQIERGYEKVDEKLIKLGA
                     RIYRETE"
     gene            complement(550..1173)
                     /locus_tag="PLT08_10415"
     CDS             complement(550..1173)
                     /locus_tag="PLT08_10415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE04921.1"
                     /translation="MIKKIIPTLALFAVLLAACGGQPAEPTLSSEQVQGTAISAAFTM
                     VAETQAAIPTNTPVPPTETPSPTPLPTFTPLPLPTQAPIEFPTATTAASSGGGGQCDS
                     VLNEAEGGPKSTVRFQNKTNGVVDLSLYMYSPNKFAQCGSLASNPYHIIKNGSVDISL
                     PKGPYYAYAWITLSNGTLTSAEGYLYNKEADNHLFYVHVKEDVIVVK"
     gene            complement(1213..1914)
                     /locus_tag="PLT08_10420"
     CDS             complement(1213..1914)
                     /locus_tag="PLT08_10420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE04922.1"
                     /translation="MKRLILVSIVLGLLLSACLPSLSQPQEYPTPVSETELQATAAIL
                     SQQTLQSIPTNTAIPTETLKIVTPTKTITPTQPTPTETVNPVLLTLTATLLAGTSPAE
                     TQTAVTKTLSTEEITGTPSLTATTVSAVTTSGTPQPAFYGTLPPNLPSGTLGLYNRDH
                     VDVYVSFRCVTKDGYVTILEYPVNKNVNVSAPAGKYTYVAWVGGRQFDGSFTLDNEGF
                     LVISFFKNKVTIKKS"
     gene            complement(1936..2283)
                     /gene="trxA"
                     /locus_tag="PLT08_10425"
     CDS             complement(1936..2283)
                     /gene="trxA"
                     /locus_tag="PLT08_10425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HNE04923.1"
                     /translation="MSGEPIHITDEAFEQVVMQSNLPVIVDFWAPWCNPCKMIAPTLD
                     KLAKELEGKIVIAKVNTDDHAQWMQKFGIQGIPTLLFVSNGKVVHRQVGALPERMLRD
                     VVNQFMEVAGQQA"
     gene            2396..>3136
                     /locus_tag="PLT08_10430"
     CDS             2396..>3136
                     /locus_tag="PLT08_10430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005047 - signal recognition particle
                     binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006614 - SRP-dependent cotranslational
                     protein targeting to membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signaling recognition particle receptor family
                     protein"
                     /protein_id="HNE04924.1"
                     /translation="MSIFSRWREGLSRTSKAAFGQIASILGTSEITDETWDDLEATLI
                     QADLGIETASSVLEDLKRLTRAEGWTRSDELFSALKAELRSRLIQPPAISLNDKPTVI
                     LVVGVNGSGKTTTIAKLSAKFVGEGKKILLGAADTFRAAAVDQLQVWGDRLNVEVIAG
                     APESDPGAVAFSAVQAGVARGMDIVMVDTAGRLHTRFNLMEELKKVNRVVGKALPGAP
                     HQVWLVLDATTGQNALQQARSFKEAVNVS"
BASE COUNT          873 a          627 c          758 g          878 t
ORIGIN      
        1 ttgggatgat aaaccaagtt tttcgtagat taggagttca ctgggatgta caggggacta
       61 atatctacgt tccagcggaa caaaatttag taattgcgtc agacttagat ggtgctatac
      121 cagaaattaa gacaaatgtc tggcctgcat ttccaacgga cctgacaagt attgcaatta
      181 cagttgcaac acaggtctca ggttctgtta tgtttcatga ttggatgttc tcaggaagaa
      241 tgtactttac agacaaacta gcgggtatgg gtgctcgcat tattttatgt gacccatatc
      301 gttgcttgat acaaggacca actcaattgt atggcgaaaa attagaaagc cccgatattc
      361 gtgctggtat ggcaatgttg ctggctgctc ttgctgctaa tgggacatca aaaattagaa
      421 atattgtcca aattgaacgc ggctacgaaa aagtggatga aaaattaatt aagcttggtg
      481 caagaatata tcgagagact gaataaaaaa gcgccgcaga agcggcgctt ttttatttta
      541 aggaaaggat tactttacta caatcacatc ttcttttacg tgaacataaa agagatgatt
      601 gtcagcttct ttattgtaga gatacccttc tgccgaagtt aatgtgccat tagatagagt
      661 aatccatgca taggcatagt aaggaccttt ggggagtgaa atatccacag aaccattttt
      721 gataatgtga taaggattgg atgcaaggct accgcactga gcaaatttat taggagaata
      781 catgtataga gacaaatcaa ctacaccatt ggtcttgttt tgaaaacgta cagtcgattt
      841 tggtccacct tcagcttcat tcaaaacact atcgcattgt ccaccaccgc cagagctggc
      901 agcagttgtt gctgtcggaa actcaatagg agcctgagta ggaagaggca aaggtgtaaa
      961 ggttggtaag ggagtagggc ttggagtttc cgtaggagga acaggtgtat tagtcggaat
     1021 tgctgcttgt gtctctgcta ccattgtgaa tgcagctgaa attgcagtgc cttgtacctg
     1081 ttcagaagag agggtgggtt cagccggctg accaccgcat gcagccaaaa gcactgcaaa
     1141 caaagccagg gttgggatta tttttttgat catattgttt actatctcca tttacttttt
     1201 cgatattgaa aactagcttt tctttatagt tactttattc ttgaaaaatg agatgactag
     1261 aaaaccctca ttgtccaagg tgaaactacc atcaaattgc ctgccgccaa cccaagcaac
     1321 atatgtatat ttcccagcag gggcgctgac gttgacattt ttatttacag gatattcaag
     1381 aattgtgaca tagccgtctt tggtcacaca acgaaacgac acataaacat cgacatggtc
     1441 tctgttataa agtcctaaag tacctgatgg tagattagga ggaagtgtgc cataaaaagc
     1501 aggttgaggt gttccgctgg tagtaacagc cgatacggtt gttgctgtta ggctaggcgt
     1561 tcctgtgatt tcttcagtcg aaagtgtttt tgtaacagcg gtttgtgttt cagctgggga
     1621 tgttcctgca agaagggttg cagtgagagt taatagaaca gggttaaccg tctcggtagg
     1681 agttggctgt gtaggtgtga ttgttttagt aggcgtaacg atttttaggg tttcagttgg
     1741 gattgctgta tttgtgggaa tggactgcaa agtttgctgt gaaagaatag cagccgtagc
     1801 ttgtagctct gtttcagaaa caggggtagg gtattcttga ggttgcgaaa gtgatgggag
     1861 acaggcactt aataacaaac ccaacacaat agaaacaaga attagcctct tcatacccta
     1921 ctccaacgtt ttaatttaag cttgctgtcc cgcaacttcc atgaattggt tgaccacatc
     1981 gcgcaacatg cgttcgggga gtgcgccaac ttggcggtga acaaccttgc catttgaaac
     2041 aaacaacaaa gttggaatac cttgaatgcc aaacttctgc atccattgag catgatcatc
     2101 tgtgttgact tttgcaataa cgatcttgcc ttctaattct ttggcaagtt tatcgagagt
     2161 aggcgcgatc atcttacaag ggttgcacca tggtgcccaa aaatccacaa taacaggaag
     2221 attggactgc atcacaacct gttcaaacgc ttcgtcagtg atatgaatgg gttctccgga
     2281 catttttatt cctttttaac ctttatgatt ctttcttatt gagccatatt tagccaaatg
     2341 agtataacat aaaaaacatg ccatcatatg cccatagggt ataatcgccg cccatatgag
     2401 cattttctca cgatggcgag aaggactttc tcgcacaagc aaagctgcat ttggtcagat
     2461 cgcttccatc cttggtactt ctgaaattac agatgaaaca tgggatgacc ttgaagccac
     2521 attgattcaa gcagacttag gaattgaaac agcatcatct gtgctggagg acttaaaacg
     2581 cctcacgcgt gccgaaggct ggacacgctc cgacgaactt ttttctgcgc tgaaagctga
     2641 actgcgttct cgcctaattc aaccgcctgc tatttccttg aacgacaaac caaccgtgat
     2701 tttggttgtg ggtgttaacg gttctggcaa gacgacaacc attgccaaac tttctgccaa
     2761 attcgttggc gaagggaaaa agatacttct tggcgcagcg gacaccttcc gggctgcggc
     2821 ggtggaccag cttcaggtgt ggggagacag gctaaacgtt gaagtgatcg ctggcgctcc
     2881 agagagtgac ccaggtgcgg tggcgtttag cgccgtccaa gctggtgtag ctcgcggcat
     2941 ggatatcgtc atggtcgata ctgccggacg cttacatacc cgcttcaacc tgatggaaga
     3001 gttgaagaaa gtgaaccgtg tagtaggtaa agcattgccc ggtgcgccgc atcaagtgtg
     3061 gcttgtgtta gatgccacaa ctgggcagaa tgctttgcaa caggcgcgtt cttttaagga
     3121 agccgtgaat gtctct
//
LOCUS       DAOQJE010000380         3132 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_20565_length_3132_cov_5.540786, whole genome shotgun sequence.
ACCESSION   DAOQJE010000380 DAOQJE010000000
VERSION     DAOQJE010000380.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3132)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3132)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3132
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20565_length_3132_cov_5.540786"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..571)
                     /locus_tag="PLT08_10435"
     CDS             complement(<1..571)
                     /locus_tag="PLT08_10435"
                     /inference="COORDINATES: protein motif:HMM:NF012428.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HNE04925.1"
                     /translation="MFGSKTQALQQRAQKVAPLGVNSNFRFWGDGITPYVEKAKGGYL
                     WDVDGKKYIDYRMAFGPIILGHAYAEVDQKVIEEIQKGILFAMTGELEIAVAEMIVEM
                     SPAIEMVRLACSGTEATMHAIRVARAYTGRDIILKFEGNYHGFHDHTLWSTYAPVEAY
                     GNRRSPIPVPASSGIPKMMRENIITLPFND"
     gene            complement(617..1123)
                     /locus_tag="PLT08_10440"
     CDS             complement(617..1123)
                     /locus_tag="PLT08_10440"
                     /inference="COORDINATES: protein motif:HMM:NF013224.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HNE04926.1"
                     /translation="MYEFDKTDIKIVNLLLEDGRMSASEMSRRMGDISERAVRYRVDR
                     MIEEGVIQISAVVNPEALGFNIKADVWLEVEADAVLEVAKKMASFENVTYVACGIGQN
                     DISIQLVARDTSEIYYFVTEVVRKVPGVRKTTTSIVPIILKDVYQWRVPERIAKELSE
                     NETVTASK"
     gene            1256..2812
                     /locus_tag="PLT08_10445"
     CDS             1256..2812
                     /locus_tag="PLT08_10445"
                     /inference="COORDINATES: protein motif:HMM:NF014156.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HNE04927.1"
                     /translation="MKISLLEIKEPKILKASEIKIELTTTPVKYYRHGWQSWSLTAWT
                     DLSPLPMQKPAIFHPLQTDAVYANEKNPHGSWLGAVELEDGKILLLGALATDTHVFLV
                     GNQLEGRSEAGEVEWLIAIGEEKTVFDEYVHQLGLRFGRVDKNHIPRVWCSWYSLYIA
                     IDEKILHESFDGLGNLPFDVLQVDDGWQNDIGDWEANEKFPSGMQALAEKIKSTGRRA
                     GLWLAPLLVAKSSRLYREHKDWLLRDEKGRFVSAGFNWGQQLYALDTTHPDVISWLVA
                     LMKLVRRWGFDYYKLDFLYAGALKGKRYKDMPREAAYRECLRYMREAMGEGSFFLTCG
                     TPILPALGMCDAIRIGPDVSHEWENYRNETLLYNFATPGTKNAIRTVVHRLWLKPLVH
                     IDPDVEYFESKENSLADEHKQQLQDLALICDFKATSDLPQWMTAQEREKVRAFLDAET
                     DSVQISRYVYKLDNRIVDFSSAVELPAPPTGFASAWGAFLGWLGNWKFVLRIIKTMDD
                     KKLKERVSRI"
     gene            complement(2843..>3132)
                     /locus_tag="PLT08_10450"
     CDS             complement(2843..>3132)
                     /locus_tag="PLT08_10450"
                     /EC_number="4.2.1.96"
                     /inference="COORDINATES: protein motif:HMM:NF002016.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008124 -
                     4-alpha-hydroxytetrahydrobiopterin dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006729 - tetrahydrobiopterin biosynthetic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="4a-hydroxytetrahydrobiopterin dehydratase"
                     /protein_id="HNE04928.1"
                     /translation="ALTNAEVTELLSQIPAWELVEVDGIRRLQRVFKLKNYAEAVAFT
                     NKLAMTAEKEDHHPLIVLEWGRVTVQWWTHVVQGLHQNDFVMAAKTNEIFG"
BASE COUNT          817 a          789 c          723 g          803 t
ORIGIN      
        1 aatcattgaa tggcagcgtg atgatattct cgcgcatcat tttgggaata cccgaagagg
       61 ctggcacagg aatgggactg cggcgattgc cgtatgcttc tactggcgca taagtagacc
      121 acaacgtgtg gtcgtggaat ccgtgataat taccctcgaa cttgagaatg atatcgcgcc
      181 cggtgtaggc acgcgccaca cggatggcat gcatggtagc ttctgtgccc gaacaggcaa
      241 gacgcaccat ctcgatggca ggcgacattt caacaatcat ctctgccacg gcaatttcaa
      301 gttcgccggt catggcaaac agaatgcctt tttgaatttc ttcgataact ttttggtcta
      361 cttcagcata ggcatgacct aaaataatcg gaccaaatgc catgcgatag tcaatgtatt
      421 tctttccatc tacatcccat aaatagccgc ccttggcttt ttcaacatag ggagtaatgc
      481 catctcccca aaaacgaaaa ttagagttca cccccagcgg tgccactttt tgcgcacgct
      541 gttggagagc ctgagtcttt gaaccgaaca tgattgctcc ttttatcggg ttgcggcgaa
      601 tcgccgcgta gcagaattac ttggatgcgg ttactgtttc gttctctgac aattctttcg
      661 ctatgcgctc tggcactctc cactggtaaa catctttgag aatgatcggt acgatcgaag
      721 ttgtggtctt gcgcacccct ggaaccttgc gcaccacttc ggtgacaaaa taatagatct
      781 ccgaagtgtc acgcgcaacc aactgaatac tgatgtcatt ctgcccaatg ccgcaggcta
      841 cgtacgtcac attttcaaaa gatgccattt tcttggcaac ttccaaaacc gcgtcggctt
      901 ctacttccag ccacacatcg gctttgatgt tgaatccgag cgcctctggg ttgaccaccg
      961 cactgatctg aatgacacct tcttcgatca ttcgatccac acggtagcga actgcccgtt
     1021 ccgaaatatc ccccatccgg cgcgacatct cagaagcgga catacgccca tcttctagaa
     1081 gcaaattgac gatttttata tcagttttgt cgaattcata cataggattt aggcttcttt
     1141 tccgaaagtg acacgcagaa tataccttac ggtattttag acgtcaaggg agcaaaattg
     1201 gcgtagttac gcattttcct ttacaatcag cgcgaaattt gatggaaact caaacatgaa
     1261 aatctctctt ttagaaatca aagaacctaa aattctcaaa gccagcgaaa tcaagatcga
     1321 gctcacaacc accccggtaa agtattaccg gcatggctgg cagtcttggt ctctcacagc
     1381 atggacggac ctctctccac tccccatgca aaaacccgcc atatttcacc cattacaaac
     1441 cgacgcggtt tatgcaaatg aaaagaatcc gcatggttca tggctaggcg ctgttgagct
     1501 tgaagatggc aaaatccttt tgcttggcgc acttgctaca gatacgcatg tgtttctggt
     1561 gggcaatcaa ctcgaagggc gaagtgaagc gggcgaagtc gaatggctta ttgctatagg
     1621 cgaagagaaa accgtcttcg atgaatacgt ccatcaacta gggttgcgat ttggcagagt
     1681 agataagaat cacattccac gtgtatggtg ctcatggtat agcctttaca tagccatcga
     1741 cgagaaaatc ttgcacgagt cttttgatgg gctcggaaac ctgccttttg atgtgctgca
     1801 agtggatgat ggttggcaga acgacattgg cgattgggaa gcgaatgaaa aatttccatc
     1861 gggtatgcag gcattggcag agaaaatcaa atccaccggg cgacgcgccg ggttatggct
     1921 cgctccatta ttagtggcaa aatcatcacg cctctatcgt gaacataagg attggttatt
     1981 gcgcgatgaa aagggacggt tcgtttcagc tggattcaac tggggtcagc aactttacgc
     2041 gctcgacacc acgcatcccg acgtaatctc gtggctggtt gcgcttatga agctggtgcg
     2101 ccgctggggc tttgactact acaaactcga ctttctctat gctggtgcgt taaaaggcaa
     2161 gcgctacaaa gacatgccgc gtgaagccgc ttatagagaa tgtctgcgtt acatgcgcga
     2221 agcaatggga gaaggttcct ttttcctaac ctgcggcacg cccatcctcc ccgccctcgg
     2281 tatgtgcgat gccatccgca tcggacccga cgtttcgcat gaatgggaaa actatcgtaa
     2341 cgaaactctc ttgtacaact tcgccacacc cggcacaaag aacgccattc gcaccgtagt
     2401 tcaccgcttg tggctcaagc ccctcgttca cattgacccc gatgtggaat attttgaatc
     2461 aaaagaaaac tctttagcag atgagcataa gcagcaattg caagaccttg ctcttatctg
     2521 cgacttcaaa gcgacgtccg acttacctca atggatgacg gctcaggagc gagaaaaagt
     2581 ccgcgcattt ctggatgctg aaaccgactc ggtgcaaatc agccgttacg tttacaaact
     2641 cgataaccgc atcgtggact tttcatccgc cgtagaactg cctgcgccgc ctacaggctt
     2701 tgcaagtgca tggggcgcct tcctcggttg gcttggtaac tggaagttcg tgctgcgaat
     2761 cataaaaacg atggatgata aaaaattaaa agaacgagtt tcaaggatat aaaataaaag
     2821 cgaccctgat gggtcgcttt tattatccga aaatttcatt tgtcttggct gccatcacaa
     2881 aatcgttctg atgcaagcct tgaaccacat gcgtccacca ttggacggta actctgcccc
     2941 attccagcac aatcagtgga tggtggtctt ctttttcagc ggtcattgcg agcttatttg
     3001 taaaagcaac agcctcagca tagtttttca gtttaaagac tcgctgtaaa cgtcggatgc
     3061 catctacttc caccaattcc catgcaggga tttgtgaaag cagttcagtg acctcagcgt
     3121 tggttaacgc tg
//
LOCUS       DAOQJE010000381         3124 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_20652_length_3124_cov_5.428152, whole genome shotgun sequence.
ACCESSION   DAOQJE010000381 DAOQJE010000000
VERSION     DAOQJE010000381.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3124)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3124)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3124
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20652_length_3124_cov_5.428152"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..842)
                     /locus_tag="PLT08_10455"
     CDS             complement(<1..842)
                     /locus_tag="PLT08_10455"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HNE04929.1"
                     /translation="MNKSTNKLYEYDALRGLSIVLLLILHSEIIGISVYGISLDPLAK
                     FMAAFLLGSFFFLGGYFMDASLQRYQNNYWGFVKARFVRIYPPYWAALAFFTLLYTLK
                     PFDTLIYILNLQIVFSPVYVKPLLTLWYISLLVVFLILFFILTAFSKQNTTKLVVFST
                     VVFFVVYFVHLKTGLFDPRFFRYYIIFFAGMCLYRFNDLWNRSLDTPVGYKLLAVFFS
                     SLFYLWALVSKLSDIHGALILAVVIFALSWIWLTLSIFKNTSIGQWKIWILLSTASYF
                     AYLV"
     gene            complement(839..1270)
                     /locus_tag="PLT08_10460"
     CDS             complement(839..1270)
                     /locus_tag="PLT08_10460"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNE04930.1"
                     /translation="MEIIPANILDLTALRKLEKESFDRDAWPMIDLIAVLTFSNVVRL
                     KAMENGNMIGFVAGDPRPRDGWGWIATIAVDSNFRRRGIGTALLHACESQLGVPRSRL
                     TVRTSNQGAINLYEKEGYRTIDVWKAYYSDGEDAIVMEKTL"
     gene            complement(1321..2229)
                     /locus_tag="PLT08_10465"
     CDS             complement(1321..2229)
                     /locus_tag="PLT08_10465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HNE04931.1"
                     /translation="MNGAFASFGLCLVGFIVLVIVIAAFNMIRVVPEYQRLVVFRLGR
                     VLEKPKGPGLVFLIPGVDRPVKVDLREQVREVPHQTSITKDNAPISIDFLWYYKVLDP
                     AQSVLQVGNFESAAAGIAATTLRAVIGGIPLDDVLSEREHINNMLRTRLDEVTERWGV
                     KVTNVEIREIIPPRDVQDAMNRQMSAERIRRAVVTESTGTREAAVNVAEGEKQSNILR
                     AEGQKQSAILQAEGERQAQLLRAQGFAQALETIFGAANKIDQKTMTLQYFETLKSMGM
                     SPSTKYIFPMEFTSMLDNFLGKDSKK"
     gene            complement(2247..2843)
                     /locus_tag="PLT08_10470"
     CDS             complement(2247..2843)
                     /locus_tag="PLT08_10470"
                     /inference="COORDINATES: protein motif:HMM:NF014062.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HNE04932.1"
                     /translation="MDFLLDPNIAYLFLLGGVVLAFLAIVTPGTGMLEVGAIFCFLLA
                     GYAVYNLPMNWWALLILLLSVVPFLYAVQKPKRELFLGVSIFLLVLGSVFLFAVDGWK
                     PAVNPLVALFSSGVVAAFLWIVVRKSIQAADARPTHDLGILVGQMGEARTAVHQEGSV
                     YINGELWSAKSVSEIPAGSHVLVVAREGFVLVVEPIKS"
     gene            2973..>3124
                     /locus_tag="PLT08_10475"
     CDS             2973..>3124
                     /locus_tag="PLT08_10475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015368882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase NAD-binding
                     domain-containing protein"
                     /protein_id="HNE04933.1"
                     /translation="MKYIKICVIGLGYIGLPTASTFAMNGIEVLGVDISPTIIETLNR
                     GEIHIH"
BASE COUNT         1041 a          749 c          582 g          752 t
ORIGIN      
        1 tgtaccaaat aagcgaaata tgacgctgta gaaagtaaaa tccaaatttt ccactgccca
       61 atacttgtat tcttgaaaat ggaaagcgtc aaccatatcc agctaagtgc aaatatcaca
      121 actgccaaaa tcaaagcccc atgaatatcg ctcaattttg acaccaaagc ccataaataa
      181 aaaagagaag aaaaaaacac agcgagcagt ttatatccca ctggcgtatc aagagaccga
      241 ttccataaat cattaaaacg ataaagacac atacctgcaa aaaatataat gtagtaccga
      301 aagaaccgtg ggtcaaacaa acctgttttt aggtgaacaa aataaactac aaaaaacact
      361 acggtagaaa aaacgaccaa tttagttgta ttttgttttg agaatgcagt cagaataaag
      421 aataatatca ggaacacaac cagtaaactg atataccaca atgtcaataa tggttttaca
      481 tatactggcg aaaaaactat ttgcaagttc aatatataaa tgagagtatc aaaaggtttt
      541 aaagtataca aaagcgtaaa aaaagccaag gctgcccaat atggagggta aattctcaca
      601 aatcgtgctt ttacaaatcc ccaataatta ttttgataac gttgcagaga agcatccata
      661 aaatatccac ctaaaaagaa aaaggagccc aacaagaatg cagccataaa tttagccaat
      721 gggtcaagtg aaataccata cacagatatt ccaataatct cagaatgcaa tatcaacaaa
      781 agcacaatac ttaatccacg caaagcatcg tattcataga gtttattggt agatttgttc
      841 atagagtttt ctccatcaca atggcatctt cgccatcaga atagtatgct ttccagacat
      901 cgatcgtacg atatccctct ttctcatata aattgatagc cccttgattc gacgttcgaa
      961 ctgtcaaccg cgaccttgga acacctagct ggctctcaca tgcatgtaac aatgccgttc
     1021 caattccgcg gcgccgaaag tttgaatcca ctgcaatggt ggcgatccag ccccacccgt
     1081 cgcgtggacg cggatcgcca gcgacaaaac cgatcatgtt gccattttcc atagctttga
     1141 ggcgcaccac attggagaat gtcaaaacgg cgatcaggtc aatcattggc catgcatcgc
     1201 ggtcaaaact ttccttctct aattttcgca gagcggtgag gtcaagaata ttggcaggga
     1261 tgatttccat aaaaaataag gtctctcttt tcgagagacc ttattgtatt acaaatcgtt
     1321 ttacttcttc gagtctttac cgaggaagtt atcgagcata ctggtaaatt ccatcgggaa
     1381 gatgtacttc gtggaaggac tcatccccat tgacttcaag gtttcgaagt attggagggt
     1441 catagttttc tggtcaatct tattggcagc gccaaaaatg gtttccaacg cctgagcaaa
     1501 accctgtgcg cgaagcaatt gagcctgtcg ttcaccttct gcttgaagaa ttgcagactg
     1561 tttctgacct tctgctcgca aaatgttaga ctgcttttca ccttctgcca cgttgactgc
     1621 agcttcacgc gtaccagtag actcggtcac aacagcacgg cgaatacgtt ctgcagacat
     1681 ctggcggttc atggcgtctt gcacatcacg cggaggaatg atttcgcgga tttcaacatt
     1741 ggttactttc acaccccagc gttcggtcac ttcatcaaga cgggtgcgga gcatgttatt
     1801 gatatgttca cgttcagaga gcacatcatc caatggaata ccaccgatga cagcacgcaa
     1861 agtagtggca gcaataccag ccgcagcaga ctcgaagtta ccaacctgca atacagactg
     1921 ggcagggtca agtaccttgt aataccacaa gaagtcaatt gagattggcg cgttatcttt
     1981 tgtgattgaa gtctgatgag gaacttcacg aacctgttcg cgcaaatcaa cttttacagg
     2041 gcgatccaca ccggggatta agaaaaccaa gcctggtccc ttgggcttct ccagcacacg
     2101 tcccaaacgg aacacgacca aacgttgata ttctggcaca acccgaatca tattgaacgc
     2161 ggcaatcaca atcactagaa cgataaaacc gaccaaacac aaaccaaaac ttgcaaatgc
     2221 accattcata ttctctcctt caaaatttac gatttgatag gttcaacaac gaggacgaac
     2281 ccctcgcgag caacaactag aacgtggctt ccagcaggaa tctcactaac actctttgcc
     2341 gaccacaatt ccccattaat atacacgctt ccttcttgat gtacagcagt tcttgcctcc
     2401 cccatctgtc ccaccaaaat acccaaatca tgcgttggtc gagcgtctgc cgcttgaatg
     2461 gactttctaa cgacaatcca aagaaacgca gctacaacac ctgacgaaaa caaagcaacc
     2521 aatgggttca ctgccggctt ccaaccatct acagcaaaca aaaacacaga gccgagcacc
     2581 aacaggaaaa ttgaaactcc aaggaataac tctcgctttg gcttttgaac tgcataaagg
     2641 aaaggcacaa cactcaaaag taaaatgagc aatgcccacc aattcatagg gagattatag
     2701 acagcataac cagccagcaa gaaacaaaaa atcgctccca cctctaacat tccggttcca
     2761 ggcgtaacaa tagccaaaaa tgctaataca actccgccaa gaaggaacaa ataggcaata
     2821 ttagggtcta aaaggaaatc catgtttatg cctccatata attatacaac agcattccta
     2881 taaagattta cgtaatgccc attgaaacgt ttcaaaccaa tttctctagc agttgtataa
     2941 tggacaggat aattactccc ataggagctc aagtgaagta cataaaaata tgtgttatcg
     3001 ggttgggata cattggtcta cccacagcat ctacattcgc aatgaatggc atcgaagttc
     3061 tcggcgtaga cattagccct accattattg aaaccttgaa ccgcggcgag atccacattc
     3121 acga
//
LOCUS       DAOQJE010000382         3123 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_20668_length_3123_cov_4.221643, whole genome shotgun sequence.
ACCESSION   DAOQJE010000382 DAOQJE010000000
VERSION     DAOQJE010000382.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3123)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3123)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3123
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20668_length_3123_cov_4.221643"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1487)
                     /locus_tag="PLT08_10480"
     CDS             complement(<1..1487)
                     /locus_tag="PLT08_10480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006306 - DNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-6 DNA methylase"
                     /protein_id="HNE04934.1"
                     /translation="MSINYSVDAAIACIRESSHLIEEGSVEGAIRQRFSAHLSRIFPA
                     EPWWVKLHGTGAEHHVRYNREGTQVGGFVDTLIGNTVIEYEKNISSPQIYSHGLDQVK
                     DYCAGLLNQGGMVEHIVGILSDTIRWRAYKIKSTPVSETPGVVLGRDHIQLEEIESIH
                     LVDADETNARKLISFLTRYLGREGARQLNAHTLEKDLGFDSEFCAQHVTRLSELVDHA
                     FVSNEMYGDLIRKLWGDFVTFIGEDFENGLFSREVYTKELYVLTLAKLLCANIIDKKA
                     LDSSSEELLSILNGNYFKMKGLGNLVEYDYFGWLNDLPYVSSLIPVATDMQRDLRAYD
                     FLTLPNEDLFGPILAQLARHSQRLLLGQEWTPSWLASALVREVFEQIPVDQDPRLVDM
                     CCGSGSMVIEVVKLTLQRFEELGVRQDESYISRLSHAVTGFDIDPLAVLLSKVNWVIS
                     AREKLEPFGTYQVTIPIYHADSLFALPPLAKNNFANPEVAVYELL"
     gene            complement(1513..>3123)
                     /locus_tag="PLT08_10485"
     CDS             complement(1513..>3123)
                     /locus_tag="PLT08_10485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE04935.1"
                     /translation="LDHLDQEDRELFTTLVNSYSGLADMAVPSWILCAALCATNGTLA
                     IILPESWWSREYSLIVRYMLFRWFKIKYIVDDMHAHWFHGAQVKTSLLVAEKIPRKRS
                     ALNWMTEDHYLHIKLSGEAFSNNSLVGNLVEGSSFDPEGDFVQLTSKWLSSGQSYKTN
                     LVDAKLVSIGKSAESLIRSSRGQKWLSKLENNTQKQIVSDKHSESFTLPSEIRHWLIK
                     YPSKTKFTTLESLGVNIGQGLRTGANSFFYVTLALDNEKKDVDVVLSPLLGGLREVIP
                     SNVAYPVVRKQSDLSEAYVVKPQDLIGRVLMIHKYAHPDDIKRDPELSMFYKTMPKGL
                     VNIINSASRVSVNGKHIWELSAVSPNTRNENKGRDTKKRFWYMLPDLSPRHNPDVFIP
                     RVNSGIPKVYLNSNRASIIDANFSTLWLSSDSKVDIWSLLALLNSSWCWAVLEYSSAI
                     MGGGALKVEAAHLRKIPMPILSKRELEHLSKLGKMFDKVDEKKIKQKIDELIISSIVG
                     DKFTVDAYNSLLSLSNEKMSQRKNHKKF"
BASE COUNT          961 a          678 c          512 g          972 t
ORIGIN      
        1 aacaaaagtt cgtaaacggc tacttcagga ttagcgaaat tatttttagc aagtggaggc
       61 aaggcaaaca gtgaatctgc atgataaatt ggaatcgtaa cttgataagt accaaaaggt
      121 tcgagttttt cacgtgcaga tatcacccaa tttactttcg acaagagaac agcaagaggg
      181 tcaatgtcaa atccagtgac cgcatgcgaa agtcgcgaaa tatatgattc atcttggcga
      241 acacccaatt cctcaaatcg ttgtagcgta agttttacta cctcaatcac cattgatcca
      301 gagccgcaac acatatcaac aagtctagga tcctgatcaa ccgggatttg ttcaaaaact
      361 tccctgacta gtgctgatgc caaccaagat ggagtccatt cctgcccaag caacaaacgc
      421 tgggagtgtc gagctaattg cgccaaaatt ggaccaaaga gatcttcgtt aggaagtgtc
      481 aagaaatcat aagcacgaag atctcgctgc atatctgttg ccactggaat aagcgaagaa
      541 acatatggca aatcattaag ccagccgaaa taatcatatt cgactaaatt ccccaatcct
      601 ttcattttaa agtaatttcc atttaatata gatagcaatt cttcgctaga actatcaagt
      661 gcttttttgt caataatatt cgcgcacaat aatttcgcta aggttaagac ataaagttcc
      721 tttgtgtaca cttctcgact aaataaccca ttttcaaaat cctccccaat gaatgtaaca
      781 aaatctcccc aaagctttct tatcaaatca ccatacatct cgtttgaaac aaaagcatga
      841 tcaactaatt cactaagtct cgtgacatgt tgcgcgcaga attcactatc aaaacctaga
      901 tctttctcca atgtgtgggc atttaattgg cgcgctcctt cccgtccaag atatcgtgtc
      961 agaaaagaaa ttaattttct agcattagtt tcatctgcgt caactaaatg tatagattca
     1021 atttcttcta attgaatatg atctctgcct aaaacaacgc caggtgtttc cgagacaggt
     1081 gtgcttttta tcttgtatgc acgccaacgt atcgtatcag aaagaatccc aacgatgtgc
     1141 tcaaccattc caccttggtt gagcaacccg gcgcaatagt cctttacttg atctaaacca
     1201 tgtgagtata tttgaggcga tgaaatattt ttctcatatt cgattacagt gttaccaatt
     1261 aatgtgtcaa caaatccacc aacttgggtt ccttctctgt tatatcttac atggtgttct
     1321 gcgccagttc catgaagctt aacccaccaa ggctcagcag gaaaaatcct tgacaaatga
     1381 gcagagaatc tttgtcttat tgcaccttca acacttcctt cctcgatcaa atggctagat
     1441 tctctaatgc aagctattgc agcatctaca ctatagttaa tactcatctt tatttcctat
     1501 gcccctttgt acttaaaatt ttttatggtt tttcctctga gacatttttt cattcgaaag
     1561 acttaataac gaattataag cgtccacagt aaatttatca cccacaatac tcgaaattat
     1621 caactcatct atcttctgtt ttattttttt ttcatcaact ttatcaaaca ttttccctaa
     1681 tttagaaagg tgctccaatt cccttttaga aagaatcggc attggtattt tacgtaagtg
     1741 tgcagcctca acttttagtg ctccgcctcc catgatcgca gatgaatact ctaaaacagc
     1801 ccagcaccaa gagctattca acaaagctaa caatgaccat atatccacct tagagtccga
     1861 acttaaccat agagttgaaa aattagcatc aattattgaa gcacgattac tatttaaata
     1921 aactttggga atgccactat ttacacgtgg gataaagaca tcaggattat gtcgtggaga
     1981 cagatcagga agcatatacc aaaatctttt ttttgtatcc ctacctttgt tttcattacg
     2041 tgtatttggc gaaacagcag acaattccca aatatgcttt ccgttgaccg acactcttga
     2101 tgcagaatta ataatattca ctaatccttt gggcattgtt ttgtaaaaca tagataattc
     2161 agggtctctt tttatgtcat caggatgcgc atatttatgt atcatcaaca cccgccctat
     2221 taaatcctgg ggcttcacta catatgcttc tgaaaggtca gactgttttc tcacaactgg
     2281 ataagctaca ttagatggga ttacttctcg caatccgcct aataaagggc ttaaaaccac
     2341 atcaacatct tttttttcat tatccaatgc tagtgttaca taaaaaaaag aatttgcacc
     2401 tgttctaagt ccttgtccta tattaactcc aagagactcc aaggttgtaa attttgtttt
     2461 tgaagggtat ttaattaacc aatgcctaat ttcacttggc aaagtaaaag attcactatg
     2521 cttatctgag acaatttgtt tttgagtatt attttccagt tttgatagcc atttttgccc
     2581 cctagaagat ctgattaagc tctcagctga ttttcctatg gacactaatt ttgcgtcaac
     2641 cagatttgtt ttatagcttt gtcctgaact taaccattta gatgtcaatt ggacaaaatc
     2701 tccttctggg tcaaaactcg atccttcaac cagattacca accaagctgt tattactaaa
     2761 tgcttcgcct gataatttga tgtgaagata gtggtcttct gtcatccagt taagagcgct
     2821 tcttttgcga gggatttttt ccgcaacaag taacgatgtt ttgacttgtg caccgtgaaa
     2881 ccaatgcgca tgcatatcat caacaatata cttaatcttg aaccaacgaa ataacatata
     2941 tcgaactata agggagtatt ctcggctcca ccaagattca ggcaaaatta ttgccagtgt
     3001 gccattagta gcacataatg cagcacaaag tatccatgat ggaactgcca tatcggcaag
     3061 cccggaatag ctatttacga gggtcgtaaa aagttctcga tcttcttggt ctaaatggtc
     3121 cag
//
LOCUS       DAOQJE010000383         3121 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_20694_length_3121_cov_4.308545, whole genome shotgun sequence.
ACCESSION   DAOQJE010000383 DAOQJE010000000
VERSION     DAOQJE010000383.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3121)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3121)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3121
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20694_length_3121_cov_4.308545"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            153..2927
                     /locus_tag="PLT08_10490"
     CDS             153..2927
                     /locus_tag="PLT08_10490"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF024754.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HNE04936.1"
                     /translation="MNYTKKQKEAIATSNQNLQIIACAGSGKTQVISARIIQILRQHS
                     DIKPGNIVAFTFTEKASGELKDRIYTLAKEELGETFGFAEMFIGTIHGYCLNLLQSPP
                     VYKYLKFGILSEIQQRLLIDRFSNKSGLVDTPLLKGGNLRKNIDSRLYQQLLDILSEG
                     GINIAKIPKDVLNAASKYHNLREEKRYLDYTTIIAEAVKTVKENSAVRENLARQVKFL
                     VVDEYQDVNPLQESLIKEFEAIGSNICVVGDDDQTIYQWRGSDVSNIISFSERYPLVK
                     EISLVDNFRSSTGIVDTAQKVIENNNPKRLSKKMKSAESQKFERGDLLALNFESSDVE
                     ATWIASKIKKMLAVAYKDKADSKERGLTFGDFAILLRSVKRDAPPIIHALEEMNIAYI
                     VAGMNGLFDTLEVQAMRNIFYYLSDFENPSTSKKITKKELENSIRDAQLGVTNANINN
                     GLNFLDAQKKKITPRISDELNLQKVYLDFLEKIELREEKITGARHENAGEVVFYNLGK
                     FSQVISDYVQIHYSTPSTDLYVNFAKFLYYQAADYYPEGWQDETYTKPDAVQILTVHQ
                     AKGMQWPVVFIPCLRQNRFPSKRMGGRSVWNIIPDVSVKNVDRYKGTTEDERRLFYVA
                     VTRAEKYLFCSFSPGDGNLYKNISIFFREFTSSEFVLTKDPDKKYRKSTNIRPRKVDV
                     NIALTFSELKYYFECPYMFKLRFLYGFNPPLVQPLGYGKSLHDMLAEIHAESIRGNIP
                     SIKDVPKLVNSHLNLPFASPPVFENLQNAAVKSLTRYLQEHQDNLDKLEHAEKVIELK
                     LSDGIVVNGRIDLIRRTDTGETIIVDFKSDERAQAEEITMKQLHVYAFGYEQLTGGQA
                     DLIEVHNLDKGGAKREIVDENVVSNTINNIVDAGRNLRENILPRLPSWEKKCAMCDVA
                     GICRTRPKGQ"
BASE COUNT         1063 a          577 c          645 g          836 t
ORIGIN      
        1 caagtgctac gcctgtgggt tttcggaatg ttagtgggtt gaaggttgca ggttgcaggt
       61 tttggggcgg acgaatgtcc gccccaaact tctaaattta gtgtagtgcc caaacttaaa
      121 tagactttgg acatgagtga attggggttc aaatgaatta cacaaaaaaa caaaaagaag
      181 caatagccac atcaaaccag aatttacaaa ttattgcctg tgctggatct ggaaaaacac
      241 aagtaatatc tgcaagaata attcaaattc taaggcagca ttcagatatt aaacctggaa
      301 atattgttgc atttacattt accgaaaaag catctgggga attaaaggat cgtatctaca
      361 cacttgcaaa agaagaactt ggagaaactt tcggcttcgc tgaaatgttc attggaacca
      421 tccatggtta ttgtctgaat ttattacagt ccccacctgt ttataaatat cttaagtttg
      481 gcatacttag cgaaatccag caaagactac ttattgaccg attcagcaac aagagtggac
      541 tggtagacac cccattactt aagggtggca atctaagaaa gaatattgac tcaaggctat
      601 atcaacaatt actggatatt ttgtcagaag gaggtatcaa cattgctaaa ataccaaaag
      661 atgtattgaa cgcagcaagt aaataccata accttagaga agaaaagaga taccttgact
      721 acacaactat tattgccgaa gccgtaaaaa cggttaaaga aaattctgca gttagggaaa
      781 acctagcccg ccaagtgaaa ttcttagttg tggacgaata ccaagacgta aatcctttgc
      841 aggaatcttt aataaaagag tttgaagcga ttgggtcaaa tatatgtgtt gttggagacg
      901 acgatcagac tatttaccaa tggcgaggca gcgatgtctc caatatcatt tcgttttcag
      961 aacgatatcc tttagtaaaa gaaatttcgc ttgtcgataa tttcagaagt agcacaggaa
     1021 ttgtggacac agctcaaaaa gtcattgaga acaacaaccc aaaaagacta tcaaaaaaaa
     1081 tgaaaagcgc ggagtctcaa aaatttgagc ggggagattt actcgcattg aacttcgaat
     1141 cttctgatgt ggaagcgact tggatcgctt caaaaataaa gaaaatgcta gccgtagcct
     1201 ataaagataa ggcggactca aaagagcgcg gtttgacctt tggtgatttc gcaatactac
     1261 tgcgtagcgt gaagcgagat gcacctccta taattcatgc acttgaagaa atgaatatag
     1321 catatattgt agctggaatg aacggtctct ttgacacact cgaagtccag gctatgagaa
     1381 acatatttta ttatttatca gatttcgaaa atccttctac aagcaaaaaa attactaaaa
     1441 aagaacttga aaactcaatt agagatgcgc aattgggagt taccaacgcg aacataaaca
     1501 acggcctcaa ttttctggac gctcagaaaa agaaaattac gcctcgaatt agtgatgaat
     1561 taaacctgca aaaagtctac cttgattttc tcgaaaaaat tgaattaaga gaggaaaaga
     1621 ttactggcgc gcggcatgaa aatgcaggcg aggttgtctt ttacaacctc ggtaaattta
     1681 gtcaagtaat atctgattac gtacaaatcc actattcaac accatctaca gatttgtatg
     1741 tgaattttgc caaatttctt tattaccaag ctgcagatta ttatccagag ggatggcaag
     1801 acgaaacata cacaaagccc gatgctgttc agatcttgac tgtgcatcaa gcaaaaggta
     1861 tgcaatggcc tgtagtattc atcccttgct tacgacaaaa cagattccca tcaaaacgaa
     1921 tgggcggaag atctgtatgg aatattatcc ctgatgtcag cgtcaaaaat gttgatagat
     1981 acaaagggac gactgaagac gaacgacgtt tgttctatgt agccgtgact cgggctgaaa
     2041 aatatttgtt ttgtagcttt tctcctggcg acggtaatct atataaaaac atatcgattt
     2101 tttttcgaga gtttacgtct agtgaatttg ttttaacaaa ggaccctgac aagaaatatc
     2161 gtaaaagcac aaatattagg cctcgaaagg tggatgtaaa tatcgcattg acctttagcg
     2221 agttaaaata ttattttgaa tgtccatata tgtttaagct tcgcttctta tacggcttca
     2281 acccgcctct tgtgcagccg ttaggatatg gaaaatcctt gcacgatatg ctagcagaaa
     2341 tacatgcaga atctattcgc ggaaatatcc catccatcaa ggatgtacct aagcttgtga
     2401 attcccattt aaaccttcct tttgcaagtc cgcctgtctt tgaaaattta caaaacgcag
     2461 ctgtgaagtc actaacaagg tatttacaag aacaccaaga taatctagac aagctggaac
     2521 atgcagagaa agttattgag cttaagttaa gtgatggaat agttgttaat gggagaatcg
     2581 accttattcg acgaactgat acaggagaaa caatcattgt cgactttaag tcggatgaac
     2641 gagctcaagc tgaagagata actatgaaac aacttcatgt gtacgcattt ggctacgaac
     2701 aattaacagg tggtcaagcc gacttaatcg aagtgcacaa tttggacaaa ggtggcgcaa
     2761 aaagagagat tgtagatgaa aatgttgtct caaacactat caacaatatt gttgatgctg
     2821 gaagaaatct acgagaaaat attctcccac gtcttccgtc ttgggaaaag aaatgtgcta
     2881 tgtgtgatgt tgcaggcatt tgtcgtacaa gaccaaaagg ccaatagcaa gtttgttttt
     2941 atgaaagagt ttgacaagca ggacacaagc gaagagatag tcacatttga ggtggctgtc
     3001 acttgtggtt attaccccaa ggtcctcatg ggtggagggg taagaatgaa agcgcctgac
     3061 tgccacattt ctccacgggt ataatcactc catgcccaac acatctcacc cactgacacg
     3121 g
//
LOCUS       DAOQJE010000384         3120 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_20704_length_3120_cov_5.899184, whole genome shotgun sequence.
ACCESSION   DAOQJE010000384 DAOQJE010000000
VERSION     DAOQJE010000384.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3120)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3120)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3120
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20704_length_3120_cov_5.899184"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..549)
                     /locus_tag="PLT08_10495"
     CDS             complement(<1..549)
                     /locus_tag="PLT08_10495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucomutase/phosphomannomutase family
                     protein"
                     /protein_id="HNE04937.1"
                     /translation="MTIHFGTDGWRAVISDTFTFSNLRMVSQAIADAVASEHWDKSEN
                     VDEKKIVIGYDTRFLSDRFAGEVGRVLAANGFSVLLAQSDSPTPAISYAVKNNNAIAG
                     VMITASHNAPRYNGVKLKGAFGGSALPEQCRRVEVYINDNEQQARGPNLMDFKKAREA
                     GLIQRFNPLPAYFDHLRKLINTD"
     gene            complement(622..1935)
                     /locus_tag="PLT08_10500"
     CDS             complement(622..1935)
                     /locus_tag="PLT08_10500"
                     /inference="COORDINATES: protein motif:HMM:NF016013.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF389 domain-containing protein"
                     /protein_id="HNE04938.1"
                     /translation="MSIPEEPNLPSFSPQDETPAPPPPIRRRRATRRSHIPTDAEGQA
                     ELIASLARRAYPSIELFVFSLVCGAILGLGYLLDSQALLLLGVLVAPLMTPWVGFLLS
                     ILSGSLRFMFETLMALLISIIFVFLGGLLTGLAALPFMPRTFDNVYLHSRLWIPELIV
                     FALGAIILVISFARSEDKPFLPSVLIAYSFYLPISAAGFGIGSGLPNVWLQGLLVFLV
                     HFAMVSIIGLITLFVLRLRPSAGGVVLSVVTLVIFGGVIAFVAGSGSPSRNDAVPAST
                     PTNVITSAVPSLTPSLVAVPNTNTTPTVKSATSTPTVSSFGSITPSPVPLTLEITLPP
                     TDTPTITLTIQPTPTYGKVQANEGGGANLRDAPGGTYVMTLLNGTIVETFSEFQVLNN
                     ITWVKVYAQVNGQRIEGWLLESVISYATPAPNFQASPTPQGTPTP"
     gene            2046..2936
                     /locus_tag="PLT08_10505"
     CDS             2046..2936
                     /locus_tag="PLT08_10505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE04939.1"
                     /translation="MKEPRVYKTSPILLVFIGLLFLFFIGILLFAIGPNSVGFMIPVG
                     VFSLFLFGAAFISMASKTIVSDDEIAVQGLLGTKNINWANVGRVSGWGYAMKLHSRDD
                     DVTLSISPRLPGYQEVIEFVGSKRPDLFSVQEYGSMKRGITQYLAVLLGLLMMGGVAV
                     AYIVAIMSNEQTITSSSFMPLLIIAVFALIMIVTTLSGPHSINLEGNSVNLKYLFSEK
                     SFQSNEIANVQLSFTQSRNGRHYFIALNLLSRKTIRISGLGMSLPIAYLVLKNWHLGR
                     STGSGAQTNIAPNWSDNTWR"
BASE COUNT          825 a          645 c          842 g          808 t
ORIGIN      
        1 gtcagtgttg atcagtttgc ggaggtggtc gaaataagca gggagcgggt tgaagcgttg
       61 aattagcccg gcttcgcgcg cctttttgaa atccattaag ttgggtccgc gggcttgttg
      121 ttcattgtca ttgatgtaca cttccacgcg gcggcattgt tcggggagtg ccgaaccacc
      181 aaaagcgccc tttaatttca cgccgttgta gcgtggtgca ttgtgtgatg ctgtgatcat
      241 cacccccgca atcgcattgt tgtttttgac agcatatgaa attgctggcg tgggggaatc
      301 tgactgtgcg agcaaaacgc taaatccatt tgcggcgagt acacgcccta cttcgccagc
      361 aaaacgatct gaaagaaaac gagtgtcgta accaattacg atttttttct catccacgtt
      421 ttcagattta tcccagtgtt ctgaagcaac tgcatctgcg atggcttgcg aaaccatacg
      481 caaattactg aaagtaaatg tatctgagat gacggcgcgc cagccgtcgg tgccaaaatg
      541 aatggtcatt gagtctcctg aagtaaagtg atatgaaaag gtgttgacgt cgtaatagcc
      601 tgtcacctta agaacatgaa tttatggggt aggtgtacct tgcggcgtgg gcgatgcctg
      661 gaagttcggt gctggggtcg cataagaaat gacagactct aacagccaac cttcaatgcg
      721 ctgtccgttt acttgggcat atactttcac ccaagtaatg ttattcaata cttggaattc
      781 agagaatgtt tctacaatcg tcccattgag cagggtcatt acataggtgc cgcctggggc
      841 gtcacgtaag tttgcaccac cgccttcatt cgcctgaact tttccgtatg taggggttgg
      901 ttgaatggta agtgtgatcg tgggcgtgtc tgtgggcggc aaagtgattt ctagggtgag
      961 tggcacaggg ctgggcgtaa tgctgccaaa agatgagaca gtgggtgtgc tcgtcgcaga
     1021 tttaacagtc ggtgtggtat ttgtattggg gacagcaacc aaacttggtg ttagagaagg
     1081 aaccgcgcta gtaatcacat tggttggtgt ggaggcagga accgcatcat ttcttgacgg
     1141 ggagcctgat cctgcaacaa aggcgatgac tccgccgaag atgaccagcg ttaccacgct
     1201 caaaacaact ccacctgcag atgggcgcag tctcaaaaca aagagcgtga taagaccaat
     1261 gatgcttacc atggcaaagt ggacaagaaa cacaagcaag ccttgcaacc acacatttgg
     1321 cagacctgag ccaatgccaa accctgcggc gctaatcggt aagtaaaacg aataagcaat
     1381 tagcacactt ggtaagaaag gtttatcttc agagcgtgcg aatgatatga caaggatgat
     1441 tgcgccgagg gcgaagacga ttaattcagg aatccacagg cgcgagtgta aatagacatt
     1501 atcaaaagta cggggcatga agggcagtgc tgccaaccca gtaagcaagc ccccaagaaa
     1561 aacaaagatg atgctgatga gcaatgccat gagcgtttcg aacatgaaac gcaaagaccc
     1621 agaaagaatg gacaagagaa agcctaccca aggcgtcatg agtggagcaa cgagaacgcc
     1681 tagtaagagc agagcttgag agtcaagtaa gtagccaagc ccaaggattg cgccacatac
     1741 caaagagaac acgaagagtt caatggaggg gtaggcgcgg cgtgcaagcg acgcaatgag
     1801 ttctgcctga ccttccgcat cggtagggat gtgagaacgg cgcgtggcgc gtctgcggcg
     1861 gatgggtggg gggggagccg gggtttcatc ctgcggcgag aaggacggca ggtttggttc
     1921 ttctgggatg gacatatggc aattatacag gaataacggg taggaaagag aggtgatgga
     1981 gtattacaat atcttcatcg tatagttggg accttgtcat tcagaactga aataggagct
     2041 ttattatgaa agaaccacgt gtgtataaga cctcaccaat ccttttggtg ttcatcggtc
     2101 ttttgttttt attctttatt ggaatactac ttttcgcaat cggaccaaat tccgttgggt
     2161 ttatgattcc agtaggtgtt ttctctttgt ttttatttgg tgcggcattt atctcaatgg
     2221 catccaagac gattgtctcg gatgatgaga tcgccgtgca gggattgttg ggcacaaaga
     2281 atattaattg ggcgaatgtt ggcagggttt cgggctgggg gtatgccatg aaacttcata
     2341 gtagagacga tgacgtgacc ttatccatta gcccgcgttt gccggggtat caagaggtga
     2401 tcgagttcgt cggttcgaag cgaccagacc tttttagtgt gcaagaatat ggcagtatga
     2461 aacgcggtat tacccaatat ctggcggtct tattggggct tcttatgatg ggtggggttg
     2521 cggtcgccta catagttgcg atcatgagta atgagcagac tataacctcc tcttcattta
     2581 tgccgctttt gattattgct gtgtttgcat tgattatgat tgtcaccacc ctctctggac
     2641 cgcattctat caatctagaa ggaaactctg tgaatttgaa gtacctgttt agcgaaaaaa
     2701 gttttcagtc taatgagatt gcaaatgtgc agctttcttt cacacaaagc cgaaatggca
     2761 ggcattattt cattgcattg aatttattga gccggaagac cattcgcatt tctggtcttg
     2821 gtatgagttt gccaattgcg tatctggtgc tgaagaattg gcatcttggg cgttctacgg
     2881 gttcaggtgc tcaaacgaat attgccccga actggtctga caatacgtgg cggtagggta
     2941 gaactgttca gcatcgcaga ctagacaatt tgcgatgctg aacggattta attcgtcaga
     3001 caaattgatg aaattaatat agattgctaa caaaccatta atcgcgtacc tgctataata
     3061 gcctttgcca ctacataacg gcacaggatg cctgacacgc gaaactctgc gcgtgacgcc
//
LOCUS       DAOQJE010000385         3120 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_20705_length_3120_cov_4.481892, whole genome shotgun sequence.
ACCESSION   DAOQJE010000385 DAOQJE010000000
VERSION     DAOQJE010000385.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3120)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3120)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3120
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20705_length_3120_cov_4.481892"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..738)
                     /locus_tag="PLT08_10510"
     CDS             complement(<1..738)
                     /locus_tag="PLT08_10510"
                     /inference="COORDINATES: protein motif:HMM:NF012770.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HNE04940.1"
                     /translation="MKKIYLGLVIILIALQACAPQNATSTPEPTTQPAATTVSETETS
                     AQDTAARLEQLGGYPCPDSDFTCVDVSVPLNHFDSQDTRTTNVVFAVLPATGERKGMF
                     VTVIGGPGGSGLMSADGYTSYFDPRLTEHFDIVFFDQRGINQSGGLYCVNAVTKYYST
                     NTDATTPEGEAQLLDVTKTFVDECIAEVGQTDLLPYLDTTQAVEDLEVFRGLMGDDKF
                     WLYGESYGTQFSQMYATAHPDHLAGLIL"
     gene            complement(853..1896)
                     /locus_tag="PLT08_10515"
     CDS             complement(853..1896)
                     /locus_tag="PLT08_10515"
                     /inference="COORDINATES: protein motif:HMM:NF012958.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase-fold protein"
                     /protein_id="HNE04941.1"
                     /translation="MTTNLLQLARANGNPVIEGNRVTFVWQGKSAPYFIDDSHQWEES
                     PQKMKSVAPGLWAYTLELDSDAYLEYSFYDPRTKKRFKDPLNKKTVFNGIEHYNHYFY
                     MPEASPTPYAKKRKGIPSGRVTRHLVNTWMLQDNGKRELHLYQPPVKGPVPLLLVYDG
                     TDYLKRANLNIIVDNLIHEKRIRPFAMAFLQNGGDRRSVEYACSDATLLDIEHSVLPF
                     AAKKLNLLDIKTNRGAYGVLGASYSGLMSVYTGLRMPDIFGKVIAQSSVFALEGRDFV
                     AVDLIRAKMSREIKIWMDIGHFDWLLEDNRRVQPLLQTHGYNVTYREAPAGHNYSFWR
                     DQLPDALEAMFGK"
     gene            complement(1975..>3120)
                     /locus_tag="PLT08_10520"
     CDS             complement(1975..>3120)
                     /locus_tag="PLT08_10520"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HNE04942.1"
                     /translation="NGERQMKTKVLVLGAGFGGLELSSILSEELGNDLDLTLIDKNDS
                     FFFGFSKFDVMFGRKTADAVRIQYRNMVKPGVTFKQEAVTAIDPAAKRVTTDKAVYEA
                     DVLVIALGADYDISHTPGLAEGGNEFYSFAGVEKLREVLPIFKKGRVLVGVTSTPFKC
                     PPAPSEAALLTHDYLLERGIRSECEISLVMPFGIPIPPSPETSKALLRAFSERGISFV
                     PDRLVRSLDASRRVATLDNGSEMPYDLFLGIPKHRVPQVVADSGLTEDGWIPVDTKTL
                     KTKFENVYAVGDVNSVGTPKAGIFAEGAARTVAAQIIAQARNTDEPAGYRGVGACYIE
                     FGAQRVGKVEVEFLAGPPTGTYTEASEELVKDKVLFGSTRRAKWFGL"
BASE COUNT          711 a          817 c          812 g          780 t
ORIGIN      
        1 gaggatgaga cccgccaaat ggtcggggtg agccgtggca tacatttgcg agaactgtgt
       61 gccgtagctt tcgccgtaca gccagaattt atcatcgccc attaacccgc ggaacacttc
      121 caagtcttct acggcttgtg tggtgtcgag gtaaggtaat aggtcggtct gaccgacttc
      181 ggcgatgcat tcatctacaa atgttttggt cacgtcgagt agttgagctt ctccttcggg
      241 cgtggtggca tcggtattcg tgctgtaata ttttgtgacc gcattgacac aatacaaacc
      301 gcccgattgg ttgatgccgc gttggtcgaa gaagacaata tcaaaatgtt cggtgaggcg
      361 cggatcaaaa taagaggtat acccatctgc gctcatcaag cccgagccgc caggtccacc
      421 gatgacggtg acgaacatgc ctttgcgttc gcccgtggca ggcaacaccg cgaagacgac
      481 attcgttgtg cgggtgtctt gcgagtcaaa atgatttaag ggcacgctga catcaacaca
      541 ggtgaaatca ctatcagggc agggataacc gccaagttgt tcgaggcgtg cagcggtatc
      601 ctgcgcggac gtttcggttt cactgaccgt tgtggcggca ggttgtgttg ttggctctgg
      661 agtggaggtt gcattttggg gtgcgcaggc ttgtaaggca attagaatga tcaccaagcc
      721 cagataaatt tttttcataa aaagcctttc ttttgataag gtcgtttata gctcaaagac
      781 ttccgccctt ttcgatatca tatcatttaa agaaaaaagg tgcatcagac ttgatacgcc
      841 tttatgggac atttacttcc cgaacatcgc ttccaacgca tccggaagtt ggtctctcca
      901 aaaggaatag ttgtgtcccg ccggagcctc gcggtaggtg acattgtagc cgtgggtttg
      961 caacaacggc tggactctgc gattatcttc caacagccaa tcgaagtgac cgatgtccat
     1021 ccatatcttg atctcacgag acatcttcgc gcggattaag tctactgcca cgaagtcccg
     1081 tccttccaag gcaaacacag acgattgtgc gatgactttg ccaaaaatat cgggcatgcg
     1141 caagccggtg tagacagaca tcaatccgct ataggatgcg cccagcacgc catacgcgcc
     1201 gcgattggtt ttgatgtcga gcaggttcaa cttcttcgcc gcaaatggca agacggaatg
     1261 ttcaatatcc agcagagtcg cgtcggagca ggcgtattcc acgctgcggc ggtcgccgcc
     1321 attttgcagg aatgccatcg cgaacggacg gatgcgcttc tcgtgaatga gattatccac
     1381 gatgatgttg agattggcgc gtttgagata atccgtcccg tcgtagacga gtaacagtgg
     1441 cacaggtcct ttgaccggcg gttggtagag atgcagttca cgtttgccgt tgtcttgcag
     1501 catccacgta ttcacgaggt gacgggtgac tcgtccgctg ggaatgccct tgcgtttttt
     1561 tgcataaggc gtggggcttg cttcaggcat gtagaagtaa tggttgtaat gctcaatgcc
     1621 gttgaagacg gtctttttgt tgagcgggtc tttgaagcgt ttcttcgtgc gcggatcgta
     1681 gaagctgtat tcgaggtagg catctgaatc cagttcaagc gtgtatgccc acaaccccgg
     1741 agcgacgctc ttcatctttt gcggactttc ctcccattga tgactgtcgt caataaaata
     1801 cggcgcggac tttccctgcc acacgaaggt gacgcgattc ccttcgatga cagggtttcc
     1861 gtttgcgcgg gcgagttgga ggaggttggt ggtcatgttg ctattgtact ggttcgaaat
     1921 aaaaatcacc ttctctggat agagaaggtg atttgcctta tgtatctttg atctctacaa
     1981 cccaaaccat ttcgcgcggc gggtcgagcc gaataggact ttatccttta ccagttcttc
     2041 agaggcttca gtatacgtcc ccgtgggtgg accggcgagg aactccactt ctactttccc
     2101 tacacgttgt gcgccgaatt caatatagca agcgcccacc ccacgatacc ccgcaggctc
     2161 atcggtgttg cgtgcctggg ctatgatctg agccgccacg gtgcgagcag cgccttcggc
     2221 gaagatgcct gccttgggcg tcccgacgct gttcacatct ccaactgcat acacattctc
     2281 gaactttgtc ttgagcgttt ttgtatctac agggatccaa ccgtcctcgg tcagcccgct
     2341 atctgcgacg acctgcggta cgcggtgttt tggaattcca aggaacaggt catatggcat
     2401 ctcactgcca ttatcaaggg tagccacccg gcgggatgca tccaacgagc gcacgagtcg
     2461 atccgggaca aatgaaatac cccgttctga aaatgctcgg agcagcgctt tggacgtctc
     2521 tggcgaaggg gggatgggaa tcccgaacgg catcaccagg ctgatctcac attccgagcg
     2581 gataccgcgc tccaacagat aatcatgggt caacaacgcc gcctcactcg gtgcaggcgg
     2641 acacttgaac ggagtcgagg tgacacccac gagcacgcgc ccctttttaa aaatcggtaa
     2701 tacctcgcgc aatttttcta cgcctgcgaa ggaatagaac tcattaccgc cttctgccag
     2761 cccgggcgtg tgactgatat cgtagtctgc acccaaggca atgaccagta catccgcttc
     2821 atacactgcc ttgtctgtgg tgacgcgttt cgcagccggg tcgatggctg tgaccgcttc
     2881 ttgcttaaac gtcactccgg gtttgaccat gttgcgatac tgaatacgaa ccgcatctgc
     2941 cgtcttacgc ccgaacatca catcgaattt tgagaaacca aagaaaaaag aatcattctt
     3001 atcgatcaag gttagatcca ggtcattccc caactcttcc gaaaggatcg aactcaactc
     3061 caacccgcca aagcctgcac ccaacactaa aacttttgtc ttcatctgcc tctctccatt
//
LOCUS       DAOQJE010000386         3115 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_20755_length_3115_cov_4.397386, whole genome shotgun sequence.
ACCESSION   DAOQJE010000386 DAOQJE010000000
VERSION     DAOQJE010000386.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3115)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3115)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3115
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20755_length_3115_cov_4.397386"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..312)
                     /locus_tag="PLT08_10525"
     CDS             complement(<1..312)
                     /locus_tag="PLT08_10525"
                     /inference="COORDINATES: protein motif:HMM:NF013499.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OB-fold nucleic acid binding domain-containing
                     protein"
                     /protein_id="HNE04943.1"
                     /translation="MTEHVSVSKISNYIGQDVILKGWVYNRTDKGKLVFLLVRDGSGF
                     IQCVAFKGDLSPELFETISHLPQESSVVITGSVREDKRAPGIPGGFEVGIKDLQVVQS
                     AG"
     gene            complement(465..2807)
                     /locus_tag="PLT08_10530"
     CDS             complement(465..2807)
                     /locus_tag="PLT08_10530"
                     /inference="COORDINATES: protein motif:HMM:NF015722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HNE04944.1"
                     /translation="MDKNRALQRLLNGKATNKEIEELKQAVASGEISIGGDMNRSVVI
                     IGSGNKVELTAEALGILKPENTTQEPVTGESPYMGLRYFDTQDADLFYGREALTGELL
                     SRVKNEPFLAIVGASGSGKSSVARAGLIPAWKRENERGAIHIITPTAHPLESLAASLT
                     RDSESVTATSTLMDDMSKEPRSLRLYVKKALGEKKLLLLIDQFEETFTLCKEPEERKA
                     FIENLLSLAEADGAARVVITLRADFYHHCFEYEGLRLTLEKHQANLGAMTQDELREAI
                     LKPAETNGWDFQPGLADLILQDIGSEPGALPLLSHALLETWKRRQGCTLTLQGYHEAG
                     GVKKAITQTAESVYAHLTSAEQTIARRIFLDLTELGENAQDTRRRVKLENLVQNKEQD
                     AVTKVLKTLTDARLVTTEEDSAEVAHEALIREWVTLSKWLDENRESLNLYRHMQKSAR
                     EWDRRGKEASELYRGARLKQLQGWVKDHGDSLSPLECEFVNTSQKVKKRERLRGAIFV
                     CISIVVLFCSVVLAVNGKFNQYFFIPVDMKDYWVTIPAGEFLMGSSDKQVIESLKTCS
                     ECNFPNEYPQHRVYVNTYEIGKYEVTNRQYAECVRAGVCAEGIFSYEYYKDLDSSPVI
                     NVNWYGAKTYCEWVGGRLPTEAEWEKAARGGLEYKLYPWGDEVPVCTKGKSNGANFSG
                     GIGCPNDVTAVGSFSANRYGVFDMAGNVSEWTSSLYMAYPNDSNDGREDMNSSADRVI
                     RGGSWVDSFDSVRSASRSWNNPIISNLFIGFRCARTPEGQ"
     gene            complement(2919..>3115)
                     /locus_tag="PLT08_10535"
     CDS             complement(2919..>3115)
                     /locus_tag="PLT08_10535"
                     /inference="COORDINATES: protein motif:HMM:NF012546.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HNE04945.1"
                     /translation="HNEHDHRCPIEQGEQAFVALKRAGVETEFVRFPEEPHGLSRVGR
                     TDRRIARLNHILRWMDKYLK"
BASE COUNT          735 a          834 c          690 g          856 t
ORIGIN      
        1 cccggccgac tggacgacct gcaggtcctt gatgccgacc tcgaacccac cggggatgcc
       61 gggcgcgcgc ttatcctcgc ggacagagcc ggtgatgacc accgacgatt cctgcggcag
      121 gtgagagatg gtctcgaaca gttcgggcga caggtcgcct ttgaaggcca cgcattgaat
      181 gaagcccgag ccatcgcgca cgagcaaaaa gaccagtttg cccttgtctg tgcggttata
      241 gacccagcct ttgaggatga catcctgccc gatgtaattt gaaatttttg aaacgctgac
      301 gtgttcggtc atgtaggggg ttcctctgaa taaatgattt tggtaattat attacaggtt
      361 gatggtcatt ccgacaaaga ccttaccgat tctataaagg gtcagatgat agtctctttt
      421 atacaacatc cacaataaag cagttattca tccttctgca tttatcattg cccttctggt
      481 gtgcgagcac aacgaaagcc gatgaagagg ttagagatta ttgggttgtt ccagctgcga
      541 ctggcagagc gtacggagtc aaagctgtcc acccacgaac cgccgcgtat cacccgatca
      601 gcggatgaat tcatatcttc tcgaccatcg ttggagtcat ttgggtaagc catatacaaa
      661 ctgcttgtcc attccgagac attgcctgcc atatcaaata ccccatacct atttgctgaa
      721 aaacttccta cagcagtgac atcatttgga caaccaatac cgccagaaaa attagctcca
      781 tttgacttac cttttgtaca taccggtacc tcatctcccc atggatagag cttatattct
      841 agtcccccac gtgctgcctt ttcccattct gcctcggtgg gcagacgtcc gcctacccat
      901 tcacaataag tcttagctcc ataccaatta acattaatta ctggtgatga atctaaatct
      961 ttataatact cataggaaaa tatgccttca gcacagacac cagctcgcac acactcagca
     1021 tactgacgat tagtgacttc gtatttcccg atttcatagg tattaacata aactcggtgt
     1081 tgaggatatt cattgggaaa attgcactct gagcatgttt tcaaagattc tattacttgt
     1141 ttatctgaac tgcccattag aaattcgcca gcaggaatag taacccaata gtccttcatg
     1201 tcaaccggaa taaaaaaata ctgattgaat tttccattta ccgccaagac aaccgaacaa
     1261 aacaacacaa caatgctaat gcacacaaaa atagctccac gtaatcgttc acgctttttc
     1321 accttttggc ttgtatttac aaactcacat tccagcgggc tgagcgagtc gccatggtcc
     1381 ttcacccagc cctgcaattg cttcaacctt gcgccgcggt ataactcgct ggcttcttta
     1441 ccccttcggt cccattctct tgcacttttt tgcatgtgac gatacaagtt caggctctcg
     1501 cgattttcat ccagccattt gctcaaggtt acccattcac gaatcaaggc ttcgtgcgcc
     1561 acttctgcgc tatcttcctc ggtggtgact aaacgtgcat cggttaaagt ctttagcacc
     1621 tttgttacag catcttgttc tttgttttgc accagatttt ccaacttgac tcggcggcga
     1681 gtatcctgcg cgttttcgcc cagttcggtt agatccaaaa atatcctacg tgcaatggtt
     1741 tgttcggcag aggttaggtg ggcatatacg ctttcagcag tttgggtgat ggctttcttc
     1801 acaccgcccg cttcgtgata gccctgcaag gtcagtgtgc atccctgcct gcgtttccat
     1861 gtttccagca gggcatgaga aagcaatggc aacgcgccgg gttcagaacc gatgtcttgc
     1921 aggatcaaat ccgccaagcc gggttgaaag tcccagccgt tggtttcggc aggcttgaga
     1981 atggcttcgc gtaactcatc ttgagtcatc gcgccgaggt tggcttggtg tttttccaac
     2041 gtcaagcgca agccttcgta ttcgaagcaa tgatgataaa agtccgcgcg taaggtgatg
     2101 accacccgtg cagcgccgtc tgcttctgcg agggataaca agttctcgat gaatgccttg
     2161 cgttcttctg gctctttgca gagggtgaag gtctcttcga attgatcgat cagcaataat
     2221 aatttctttt cccccaaagc cttttttaca tacaacctca aactgcgggg ttctttggac
     2281 atatcgtcca tcaatgtgga tgtcgccgtg accgattccg aatcgcgcgt cagactcgcc
     2341 gcgaggcttt ccaacggatg agccgtgggt gtgatgatgt ggatcgctcc gcgctcgttc
     2401 tcccgcttcc atgcagggat caatcccgcc cgtgccacag acgattttcc gctgcccgat
     2461 gcgcccacaa tggcaaggaa gggttcgttt ttaacacgcg ataacagttc accggtcaat
     2521 gcctcgcgcc catagaacaa gtccgcatcc tgggtatcga agtagcgcaa gcccatatag
     2581 ggcgactcgc ccgtgacggg ttcttgagta gtgttttcag gttttaaaat gccaagcgct
     2641 tctgctgtca gttcaacttt attcccactt ccgataatta caaccgagcg gttcatatcc
     2701 ccgccgattg aaatttcacc gctggcaaca gcctgcttta actcttcaat ttctttattt
     2761 gtggctttgc catttaatag tctttgcagg gccctgtttt tatccatgaa tcaccttgtt
     2821 agtcagatta taccgccact ctaattcaaa aaggtgacag tcatcttttg cgacatcgcc
     2881 ccgtagcggg caagatgact gtcacctctc tttcaaatct acttcaaata cttatccatc
     2941 cacctcaaga tgtgattcaa ccgcgcaatg cgccgatctg ttcttccgac gcgtgaaagc
     3001 ccatgcggtt cttcagggaa gcgcacgaac tccgtctcca cgccagcgcg tttgagtgct
     3061 acgaaggctt gctcgccttg ctcgatggga cagcgatggt catgctcgtt gtgga
//
LOCUS       DAOQJE010000387         3112 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_20791_length_3112_cov_5.262349, whole genome shotgun sequence.
ACCESSION   DAOQJE010000387 DAOQJE010000000
VERSION     DAOQJE010000387.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3112)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3112)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3112
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20791_length_3112_cov_5.262349"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..214)
                     /locus_tag="PLT08_10540"
     CDS             complement(<1..214)
                     /locus_tag="PLT08_10540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE04946.1"
                     /translation="MKLKYLLQTLVTLAVFTYNIQSNLIPTPEQDVSIKTNTPTVVSP
                     LTQLPPPTPPEGDTPPRHNNSCRLPHF"
     gene            complement(204..1331)
                     /locus_tag="PLT08_10545"
     CDS             complement(204..1331)
                     /locus_tag="PLT08_10545"
                     /inference="COORDINATES: protein motif:HMM:NF021134.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016758 - hexosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase 87 family protein"
                     /protein_id="HNE04947.1"
                     /translation="MRTSKEFKQRLTFALLFSFGLLLRVLFIPAKTLDMGSYILWYDY
                     IVKNGLGAIGSNYFGYNPPFIYLLALTTLTHSFLHPVIAIKLIPFIFDILSAILVYHI
                     VRMQEHEEKKPLLAALIFWIAPTIMINSSFWGQTDAIYTFFLLFTTFFLLKERPIFAL
                     LAFGIAFSIKAQAIFFAPFLGVLFFKKKIPFKAFLTFPVVYAISFIPAIIAGRPIATL
                     FSTYGGQTETFASASKNAANPYFFVNEQDYLTILYIGLVIAVTLLVTWVLIYSFKKYE
                     ITKPNLIITALNSLAIVPYLLPKMHDRYFYPADVFSIICAFFLPRFWPVAIAYQAISF
                     LSYLPYLFNTPAEATVSIAFVINTITIIFLIQKQWAITNET"
     gene            complement(1359..>3112)
                     /locus_tag="PLT08_10550"
     CDS             complement(1359..>3112)
                     /locus_tag="PLT08_10550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE04948.1"
                     /translation="PIFLTLETAQYYMAHLVRPLIEERGYFFSVTINRNRLYSQIIDN
                     LNKSAIVGIPYTEIGARLDAAWFGDPAQRRVYADAQECVTLFRQYCLEHNLLDFSLQM
                     EVFKNHLWQNEQARSYLIQTYRHLVYDNVEEDVSSSHDILDQWVPEFESALLLYDVGA
                     GYKKFLGGDPASGYALSDRCDEVFELNESFVSSPNVIQLSKALVSAINGQGEIEADGT
                     QPAMEFIMTHFHPEMLDEVVTKIRLLLEAGTPPSEVVILAPYLSDSLRFSISNRLDSA
                     GIPWRSNRPSRSLRDEPASKALLTLSALAHPQWNVHPSKFDVAHALMFALGTDLIRSQ
                     LLTDIVYGQRELRLSSFDQINPETQERITYALGARYATLREWIETYRNEAPLPLDFFL
                     RRLFGEVLSQPDFGFHTNLDAVRIAASLVESIKKFRNAMEPLQTLNDFDLGMEYITML
                     QDGVIAAQYLESWRSEDKEAVLVAPAYTFLMMNRPVAFQFWLDPGSDGWSQRVSQPLT
                     HPYILSRYWDSSSGRVWLDSDEVEMEIETLSRLVGGLLARCREKVFLALSDLGAAGFE
                     QRGALLRAFQKVLQRQQ"
BASE COUNT          968 a          585 c          792 g          767 t
ORIGIN      
        1 ggaaatgggg caacctgcaa gagttgttgt gccggggagg agtgtcccct tcaggcggag
       61 tgggaggcgg cagttgtgtg agaggagata cgacggttgg agtgttggtt tttatagaaa
      121 catcctgttc aggtgtgggt atcaaattag attggatgtt ataagtaaag accgcaagag
      181 taaccagagt ttgtaggagg tatttaagtt tcattagtaa ttgcccactg tttttgaatt
      241 aggaaaataa tagtaatagt gttaataaca aatgcaatcg aaactgtagc ctctgctggg
      301 gtgttgaaca aatatggcag ataagagagg aaggatattg cctgataagc aatagctaca
      361 ggccaaaatc ttggcaagaa gaatgcacaa atgattgaga atacatctgc cggataaaaa
      421 taacgatcat gcattttggg taagagatat gggactattg caagtgagtt cagtgcggta
      481 atgatcaaat ttggttttgt gatctcgtat tttttgaagc tataaattaa tacccatgtc
      541 actaaaagtg ttacagcgat aacaagtcca atataaagaa tagttaagta gtcttgctcg
      601 ttaacaaaaa aatatgggtt cgccgcattt ttcgacgcag aggcgaaagt ctctgtttgt
      661 ccgccatatg ttgaaaataa tgttgcaata ggtcttcctg caatgatagc aggtataaaa
      721 gaaatagcat atacaacagg gaaagtaaga aatgccttaa aaggaatttt ctttttaaag
      781 aagagaacac caaggaaggg agcaaaaaaa atggcttgcg ccttaattga gaaggctatc
      841 ccgaatgcaa ggagagcgaa aataggtctt tctttgagaa gaaaaaatgt tgtgaataaa
      901 aggaagaaag tgtaaatcgc atcggtttgc ccccaaaagc tactgttaat cataatagtt
      961 ggtgcaatcc agaaaatgag ggctgcaagc aaaggttttt tctcttcgtg ttcttgcatg
     1021 cggacaatat gataaacaag tattgcactg agaatatcga atataaatgg gataagctta
     1081 atggcaataa cgggatgtag aaatgaatga gtaagtgttg taagtgccaa caaataaata
     1141 aatggcggat tgtagccaaa ataattacta ccaatagccc caagcccatt tttgactatg
     1201 tagtcatacc aaaggatata tgagcccatg tcgagtgttt tggcaggaat gaacaatacc
     1261 cttagcaaga gtccgaacga aaaaagtaac gcaaaggtta agcgctgttt gaattcttta
     1321 gatgttcgca tgaagagcct cataaaagga aataaagttt attgttgtcg ttgtaacacc
     1381 ttctgaaatg cccgtaataa cgcaccacgt tgttcaaacc ctgcggcacc aagatcagat
     1441 aatgccaaaa ataccttttc acgacaacgg gcgagcaaac cgccaacgag gcgggatagg
     1501 gtttcaatct ccatttcgac ttcatccgag tcaagccaaa cgcgcccaga agacgaatcc
     1561 caatagcgcg aaaggatata gggatgggtg agcggctgtg acacgcgctg tgaccagcca
     1621 tcagaaccag ggtccagcca gaattggaag gcgacggggc ggttcatcat taggaaggta
     1681 tatgctgggg cgaccaaaac ggcttcttta tcttcactgc gccatgattc gagatattgg
     1741 gctgcaatga ccccatcttg cagcatggtg atgtattcca tgccaaggtc aaagtcgttt
     1801 agagtttgaa gcggctccat ggcgttgcgg aactttttga tcgattcgac aaggctggcg
     1861 gcgatacgaa ccgcatcgag gttggtatga aaaccaaaat caggttgtga gagcacttcg
     1921 ccaaataagc ggcgaaggaa aaaatcgagc ggaagtggcg cttcgttgcg ataagtttca
     1981 atccattcgc gcaacgttgc gtagcgagcc cccaaagcgt aggtaatacg ttcttgggtt
     2041 tcagggttga tctggtcaaa acttgaaagc cttaactcac gttgaccata cacaatatcg
     2101 gtgagcaact gcgagcggat gaggtctgtg ccaagggcaa acatcaaagc atgggcaacg
     2161 tcaaactttg atggatgaac attccactgc gggtgagcaa gagccgataa agtgagcaag
     2221 gctttgctgg cgggttcatc gcgcaaagaa cgagatggac gattgctgcg ccagggaatc
     2281 ccagcagaat ctaaacgatt cgaaatcgag aatcgtagcg agtctgatag atatggcgca
     2341 agaattacta cttcagaagg cggggtgccg gcttcaagca acaggcgaat ttttgtgaca
     2401 acttcatcca acatctcagg atggaagtgt gtcatgatga attccatcgc aggttgggtg
     2461 ccatccgctt caatttcacc ttgaccattg atagctgaga caagtgcctt tgagagttga
     2521 atcacatttg gcgacgagac aaaggactcg ttcaactcaa atacttcatc gcatctgtcg
     2581 ctcagggcgt agccgctggc tgggtctcca cccagaaatt ttttatagcc tgcgcctacg
     2641 tcgtaaagca aaagcgcgga ctcaaattca ggtacccatt ggtcgagaat atcatgagaa
     2701 gacgagacat cttcttcgac attgtcataa acaagatgac gataggtttg aataagatac
     2761 gaacgagctt gttcgttttg ccagaggtga ttcttgaaga cctccatttg aagtgagaaa
     2821 tctagcaaat tatgttcaag gcaatattga cggaacaagg tgacacattc ttgcgcatct
     2881 gcatataccc ggcgctgggc agggtcaccg aaccaagcgg catctaagcg agcgccaatt
     2941 tcggtatatg gaatgccaac gatggcagat ttattgaggt tatcgataat ctgggaatag
     3001 agacggttgc ggttgatggt gacagagaaa aagtagccgc gttcttcaat taatgggcgt
     3061 accaaatgcg ccatgtaata ctgggcggtt tctaatgtaa gaaagatagg gg
//
LOCUS       DAOQJE010000388         3109 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_20828_length_3109_cov_6.409299, whole genome shotgun sequence.
ACCESSION   DAOQJE010000388 DAOQJE010000000
VERSION     DAOQJE010000388.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3109)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3109)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3109
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20828_length_3109_cov_6.409299"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            29..592
                     /locus_tag="PLT08_10555"
     CDS             29..592
                     /locus_tag="PLT08_10555"
                     /inference="COORDINATES: protein motif:HMM:NF021886.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HNE04949.1"
                     /translation="MEDLIFMCSMVRRPLGMELALLGFLLRGPQHGYQLHQMVSDPSG
                     LGLIWNLKQSHLYALLSKLESDEYVISTLQNQDPHPPRKMFALTDAGRGIFFDWLTSP
                     VTAPRLIRQDFFAKFFFAQRENKDTLQKLIETQRDVCQNWVDVFKVKMEGSEQMSYNW
                     LMYQFRLGQVQAIQSWLNSCEKTLNTP"
     gene            1807..2295
                     /locus_tag="PLT08_10560"
     CDS             1807..2295
                     /locus_tag="PLT08_10560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE04950.1"
                     /translation="MKKYYLLLLTLALLLPTAALAEGQFDYIVIRGFGIVEDINISNP
                     ALTQDYYAFADFSKGDVNPPSDPGIGYQVVRMHAKDSKGIPYDQLHYYPDSGYVFYDG
                     IVNGYSELGTKWYAANPTVEKPFRAALAENARLTFIPLGVFIVLLIGFLVFYLKKPKQ
                     TA"
     gene            complement(2381..>3109)
                     /locus_tag="PLT08_10565"
     CDS             complement(2381..>3109)
                     /locus_tag="PLT08_10565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE04951.1"
                     /translation="EGARDVVLLAPPYDDGVFREDAHWIANRLRARPALVTKELHWLE
                     TTPGEAKSVLNFLNDLHRAETQAKKSAPVLPVVKERSKLMPSLVSALIGTLILFAVFA
                     VALPLDVPAGMSAAQGSAIRVALDLKGKISTANIPEGMTLPEGADAEKIFGGTHYPAS
                     IRLIVDGETVLEETYQPSGVSGNGRISALEFISIPSGSHQVEMLLKDDDNDFRTVYSD
                     TLVLDVSQVVVFSYDNSSDTVTVR"
BASE COUNT          867 a          701 c          643 g          898 t
ORIGIN      
        1 tcattcattg tgtattcatt aagtgaatat ggaggatttg atttttatgt gctcaatggt
       61 tcgacgtccc cttggtatgg aactggcctt gcttggtttt ttacttcgag ggccacagca
      121 tggctatcaa cttcatcaaa tggtatctga tccctctggg ttgggattga tttggaattt
      181 aaagcagagt catctctatg cactcttaag taaattggag tcagatgaat atgtcattag
      241 tactcttcaa aatcaagatc ctcatcctcc acggaagatg tttgcactta cagatgctgg
      301 cagagggatt ttctttgact ggttgaccag cccagttact gcgccacgtc ttatccggca
      361 ggattttttt gccaagtttt tttttgcgca acgtgaaaat aaagatactc tacaaaagct
      421 aatcgaaacg cagcgtgacg tttgccagaa ctgggtagac gtgttcaagg taaagatgga
      481 aggtagtgag cagatgtcat acaactggct aatgtatcaa tttcgccttg gacaagtcca
      541 agcaatacaa agttggctga attcatgtga gaaaactttg aatacacctt aaattatcag
      601 ttctattgtg tttgtcaaaa aaataattca gaaatcttct aagtgcaaca ggttttatga
      661 aatctgattt ctcgaaacat attaggaaat tggcaaaact atatgcagaa tttaataaaa
      721 tatttagtaa cccacaacgt gtcttaattt tgtggctctt atccgattca gaaaaaactg
      781 taagtgaaat agcaaaggct ataggggcat cgcgtccacg tacatcccag caccttcttc
      841 tcatgaagac caataatatt cttgcatccc gtcgcgaccg aaaaaatatc tattaccgta
      901 ttgtttacaa taaatttctt caagactacc tttccttgct tggaggacca gaagatgata
      961 gcgtacataa tccccttaag ttgtagatat gcatctgctt gttcctaaaa atgtaaaact
     1021 tatcagcgac agatcgaatg aaacacttaa ggttttgttt ctgttgatgt tgatgttgat
     1081 gttgatgttg attttctgtt tggcaataat tcaaaataat cataatgagt ataggttggc
     1141 agcaaagaat ttcaggttgc caacctgccc gatatagaat tacgtcttgt gccgtgaata
     1201 aaacggtcta gagatacagg cctaacatgg atttgaagtt cttttgcagt ctgaattatg
     1261 acctatccct ttcggcgctt gatgcatttg cgcaatactt gtgtgacgtt catccacatg
     1321 gtatgttttt cgctttgaga ggttgaaact ctttttactt tgaaatatcc cccacctcta
     1381 ctgagtggat tgaaactacc ttttctgcaa aaagtcggac ctgtccagag tggcatatat
     1441 catgtatatg ggtaaatttc gcgccgcctg gaatatgccc ccagataagc aaaaacgaaa
     1501 acgcctctat tcataggtgc gttttcgttt aaaccatttc cattccataa agtcctgtct
     1561 aaggtatgtg gttgtgattg gaggctgtta gttctggaag tattttataa aaacccaggt
     1621 acgatcaatc atttattatt aatcattctt ttttcattgg aattagttct ttttttacct
     1681 ataaaattac tttgatgtga aatgaagccg aaccataacc caattccctg tacaaaaaag
     1741 gatcaaacac gaatattagt aagcagtttg gcttacttac aacctaccat ccaaccttgg
     1801 agacttatga aaaaatatta ccttctcttg ctaactctgg cattgttgct ccccactgcc
     1861 gcccttgccg agggacaatt tgactatatc gtcattcgcg ggtttggaat tgttgaagat
     1921 atcaacatct ccaacccagc gctgacacag gactattacg cctttgccga tttttcaaag
     1981 ggagatgtca acccgccctc agaccctggg atcggttatc aagtagtacg catgcatgcc
     2041 aaagatagca agggcattcc gtatgatcag ttacattatt accccgatag cggatatgtc
     2101 ttttatgacg gcattgtgaa tggctattct gaattgggta caaaatggta tgcagccaac
     2161 cccacagttg aaaaaccttt ccgtgctgca ttggctgaaa acgcacggtt gacctttatt
     2221 ccgcttggcg tgtttattgt cttgctaatt ggtttcctcg tgttctatct gaagaagccc
     2281 aaacaaaccg cataaaacaa tatcatcaca gagaccacag tgaacacgga gatttaaaaa
     2341 tattctctgt gaaccctgtg gtctctgtgg tcaagattct ttatctcacc gtcactgtat
     2401 cactgctatt gtcataacta aacacaacca cctgcgaaac atccagcacg agcgtatctg
     2461 aataaaccgt gcggaaatcg ttatcgtcat ccttgagcaa catttcaact tgatgcgagc
     2521 cagatggaat ggatataaat tccagcgcag agatgcgccc gttgccgctg acacccgatg
     2581 gttgataggt ttcttccaac acggtttcac catcaacaat caaacgaatg gaagcagggt
     2641 aatgcgtgcc gccgaaaatc ttttccgcgt ctgcgccttc gggcagggtc atgccttcgg
     2701 gaatattggc agtggaaatt ttccctttca aatccaacgc cacgcggatg gcagagcctt
     2761 gcgccgcaga catcccagcg gggacgtcca acggaagcgc aaccgcaaac accgcaaaca
     2821 aaatcagcgt gccgatcaac gcgctcacca gcgagggcat caacttgctg cgttccttca
     2881 caacaggcaa cacaggcgcg gacttcttcg cctgcgtttc agcacggtgc aaatcgttga
     2941 gaaaattcaa caccgatttc gcttcacctg gcgtcgtttc gagccagtgc aattccttcg
     3001 tcaccagcgc aggtcgcgca cgcaggcggt tcgcaatcca atgcgcgtct tcgcggaaca
     3061 cgccatcgtc atacggcgga gccagcaaca cgacatcgcg cgcgccttc
//
LOCUS       DAOQJE010000389         3093 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_21004_length_3093_cov_4.843976, whole genome shotgun sequence.
ACCESSION   DAOQJE010000389 DAOQJE010000000
VERSION     DAOQJE010000389.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3093)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3093)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3093
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21004_length_3093_cov_4.843976"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..222
                     /locus_tag="PLT08_10570"
     CDS             <1..222
                     /locus_tag="PLT08_10570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE04952.1"
                     /translation="KTIDTSPYLTTGAEDMGYMQEKASGCYFFIGSANPEKNLDHIHH
                     HPKFDFDEQALISGVALMASAAANLLKEK"
     gene            224..1141
                     /locus_tag="PLT08_10575"
     CDS             224..1141
                     /locus_tag="PLT08_10575"
                     /inference="COORDINATES: protein motif:HMM:NF016648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FecR family protein"
                     /protein_id="HNE04953.1"
                     /translation="MKSTLSALLLLTLWLSACTSSPANTPSIANDPTVNVPVESTPAE
                     SNNQASAEREAVVSEVENQVAVKTTADGEFIPAEKGVVLPQGGVLQTGETGRARLDLN
                     PEGTIVRVAPNSSFTLPQISEVDGAPKTSIELFFGKIFILLNGGSVDVETPSGVASVR
                     GSLLSVSFNPATQRIQATCLEGHCGLVNASGEDVDLVEGESAYIDEDGALHELAGIDQ
                     DEIQAWLDEAPELNQFLNELPDPQDYPEFQEFDVYDFDPSAYFQENPSEADGFYSFDE
                     GTTGSGSDPNEPPATEPPSDDGSGDSSGG"
     gene            1144..1713
                     /locus_tag="PLT08_10580"
     CDS             1144..1713
                     /locus_tag="PLT08_10580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE04954.1"
                     /translation="MKRTSKWAIGVVVTVTAAFAALQTVSAPTHQTETPPAETESPIL
                     TEVVATQPCGYQWAYQDDLELTASFDASIKQLDPNASGRAQLFGENCVRQDGTFTFSA
                     LETDFYVRTPVADLKQEEEFGNWVKHVMEIVLQIPREELAIQYGFVEFHFEKTETEQL
                     AFRVPIKTYIDEAQSKTGAELFQMFYTQP"
     gene            1821..2351
                     /locus_tag="PLT08_10585"
     CDS             1821..2351
                     /locus_tag="PLT08_10585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE04955.1"
                     /translation="MTTKLPQLKQDFVESLSQISRFWGFPKGMGAIFAVLYLSPTPLA
                     LDEITVQTGLTKGAVSTEVRTLARMGLVHRSTKLGDRKDYYEAETDFYTSIRSILKER
                     QNSEFDRALRGVRSSLEQLESGAVTGSKEDIAFLRERIKALQDFFDAIDSLTNAVIKL
                     EKLGLSNVSKILNVLK"
     gene            2364..2717
                     /locus_tag="PLT08_10590"
     CDS             2364..2717
                     /locus_tag="PLT08_10590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE04956.1"
                     /translation="MNTIISFLIQMLLTITLTTIIVSYIRPYLRKVLIDLCGTEERAQ
                     FWTVFSNVLLIGLPSILALNYKPQANTLEELFFEVASRLSGTLAGFLFGLVCVGIVVS
                     VFALFAPRPVKAETK"
     gene            2714..>3093
                     /locus_tag="PLT08_10595"
     CDS             2714..>3093
                     /locus_tag="PLT08_10595"
                     /inference="COORDINATES: protein motif:HMM:NF017206.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HNE04957.1"
                     /translation="MKIAVTGAFSYSGKYIAKRLLEKGEEVITLTNHPNRPNPFNGKI
                     QAFPLNFANETELTQNLQGVDVLVNTYWVRFDKGTNTQPQAVENTKALVRAAVKAGVK
                     RIVHISIANPSADSHLPYYWGKAA"
BASE COUNT          849 a          765 c          702 g          777 t
ORIGIN      
        1 aaaacaatcg atacctcacc ctatctcaca accggcgcag aagatatggg gtacatgcaa
       61 gagaaagcca gcggttgcta cttcttcatt ggctccgcaa acccggaaaa gaacctcgac
      121 cacattcacc accaccccaa attcgacttc gatgagcaag ccctgatcag cggagtggca
      181 ttgatggcgt ctgctgctgc caatttgttg aaagagaaat aaattgaaat caaccctctc
      241 cgcgcttctt cttctgacgt tatggctgag tgcctgtacc tcaagcccag ccaacacccc
      301 atccattgca aacgacccca cggttaatgt tccagtggag tccacaccgg cagaaagtaa
      361 taatcaggca tcggcggagc gtgaggcggt tgtctccgaa gttgagaatc aagtagcggt
      421 taaaacaact gccgatggag agttcatccc ggcagaaaaa ggggttgttt tgccacaagg
      481 cggcgttttg caaactggag aaaccgggcg ggctcgtttg gatctaaacc ctgaagggac
      541 cattgttcgc gtagctccaa attcttcatt tacgttgcct caaatttcag aagtagatgg
      601 tgctcccaag accagtattg agcttttctt tggcaagatc tttatcttat taaatggcgg
      661 ctcggttgat gtagaaacgc catcgggcgt ggcatcggta cgcggtagtt tgttgagtgt
      721 cagtttcaac ccggcaacgc aacgtattca agctacgtgt ttggaaggtc actgcggact
      781 cgtcaacgca agcggtgaag atgttgatct ggtagaaggc gagtctgctt acattgatga
      841 agatggggcg ctccatgagt tggctggcat tgaccaggat gagattcaag cttggttgga
      901 tgaagcccca gaattgaatc agttcttaaa tgaactacca gaccctcaag attaccctga
      961 gtttcaggaa tttgatgtgt atgattttga cccttctgca tactttcaag aaaatcccag
     1021 tgaagcagat ggcttctact ctttcgatga aggtacaact ggttcaggca gtgacccgaa
     1081 cgagccacct gctactgagc ctccttcaga tgatggcagc ggcgactcta gcggagggta
     1141 gctgtgaaac gcacaagcaa atgggcaatt ggggttgtag taacggtcac agctgctttt
     1201 gctgccttac aaacagtctc tgcacctacc caccaaacag aaacacctcc tgctgaaaca
     1261 gaaagcccta ttctcacaga agtcgttgct actcaaccat gtgggtatca atgggcatat
     1321 caagatgacc tcgaattgac tgcatcgttt gatgcatcta tcaaacaatt agaccccaat
     1381 gccagtgggc gcgcccagct ttttggcgaa aattgtgtgc gtcaagatgg aaccttcact
     1441 ttctctgctc tagaaacaga tttttacgtc cgcacgccag tagcagattt gaagcaagaa
     1501 gaagagtttg ggaattgggt caaacacgtt atggaaatcg tgttgcaaat tccgcgtgaa
     1561 gagttggcaa ttcaatatgg gttcgtagaa ttccactttg agaaaaccga aactgaacaa
     1621 ctcgcctttc gagtgcccat taaaacctat atcgatgagg cacaaagtaa gaccggtgct
     1681 gagttattcc agatgtttta cacccaaccc taatcaaaaa cgaaaccgcc tttgaaggcg
     1741 gtttttttgt ccttgacaag ctcatcaaac ggtcatatac tgcgtttagt aattactaaa
     1801 cgttctaaaa cgaggaatat atgaccacga aactccccca actcaaacaa gattttgtcg
     1861 aaagcctgag ccagatcagt cgcttttggg gctttcccaa agggatgggc gcgatcttcg
     1921 ccgtcttata tctctcccct actccgttgg cactggatga aattacggtt cagaccggac
     1981 tcaccaaggg tgccgtcagc accgaggtac gaaccctggc gcgcatgggt ttggtccacc
     2041 gctctacgaa acttggtgat cgcaaagatt actacgaggc tgaaactgat ttctatacct
     2101 cgatccgctc cattctgaaa gaacgtcaga acagcgaatt tgaccgggca ctacgcggag
     2161 tacgctcttc ccttgaacag cttgaatctg gcgctgtcac tggaagcaaa gaagacattg
     2221 cttttctccg tgaacgcatc aaagccttac aagatttttt tgatgccata gacagcctaa
     2281 ccaacgccgt catcaagctg gaaaaactcg gtctttctaa cgtctctaaa attcttaatg
     2341 tcttgaaata aaaggaaata accatgaata cgattataag ttttctcatt caaatgctac
     2401 tcactattac cctcaccact attatcgtga gttacatccg tccatattta cgcaaagtac
     2461 tcattgacct gtgcggtacc gaagaacgcg ctcaattctg gaccgtcttc tccaatgttc
     2521 tgctgatcgg tctgccgagc atccttgctt tgaactacaa accccaagcc aacaccttgg
     2581 aagaattgtt ctttgaagta gccagccgcc tcagcggcac cttagccggg ttcttgttcg
     2641 ggctcgtttg tgtaggtatt gtcgtttccg tgttcgcctt attcgctcct cgcccagtga
     2701 aagcagagac aaaatgaaaa ttgcagtgac aggcgctttt agctactcag gcaaatacat
     2761 tgccaaacgt ttacttgaaa agggtgaaga ggtcattact ctcaccaatc accccaaccg
     2821 tcccaatcct ttcaatggga agatacaagc cttcccgctg aactttgcca acgaaactga
     2881 attaacccaa aacttgcaag gcgtagatgt tttggtcaac acatattggg tgcgctttga
     2941 taaagggacg aatacacagc ctcaagcggt tgaaaacaca aaggcattgg ttagagccgc
     3001 ggtaaaagct ggcgtcaaac gcatcgttca tatcagcatc gcaaacccat ctgcagattc
     3061 gcatctgcct tattattggg gaaaagctgc caa
//
LOCUS       DAOQJE010000390         3092 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_21011_length_3092_cov_6.306553, whole genome shotgun sequence.
ACCESSION   DAOQJE010000390 DAOQJE010000000
VERSION     DAOQJE010000390.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3092)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3092)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3092
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21011_length_3092_cov_6.306553"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(1..2199)
                     /locus_tag="PLT08_10600"
     CDS             complement(1..2199)
                     /locus_tag="PLT08_10600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE04958.1"
                     /translation="MESQPYIPGFKPVDVGPLSRFLPALEDGTISGWLSGQTLAGNWL
                     LDPFGFSPKLVLEAARSGYRVLVTANNPITRFLLEVFANPPPQADFVAALADLGAVKK
                     GDERLEQHLQSLYLTQCEKCEKQIYAQAFLWRKGEDVPYAKLYECPHCKDSGERITTD
                     SDKDIALQIAGTDTLHRTRLFERVVSLKDEDRVYAEEAIEHYLPRPLYVLGTILNRLD
                     SLDLNESRRRALTALLLLAFDAGNTLWGHPAERPRPKLLNTPSQFRENNLWTMLERGI
                     RLFAESGSPVVVEAWPKKIPETGGICIYEGRLKDLAHEVKREIPIAAVVASIPRPNQA
                     FWTLSALWAGWLWGSDAVEPYKVALRRRRYDWAWSATALFAMFSHLDELLADGVPFFG
                     LMPEPEAPFMTSALTAASAAGFKLESVALRTEHDPVQVLWKSGKKSASAEIEVETARN
                     AMRSYIAARGEPAGYLHLHTAGLIALAEVNALKQANDEFDFAMRKTQSVMEQALKDSH
                     EFVHYSTGEGVDTGVWSLASGHAESLSDKIEVGIVNYLQKNPQTIFLEVEEELNKQFT
                     GLMTPSKGLIYAILNSYAEKDGGIWNLRNEDYASARRDEMKTVFALLEEIGKRLEYKT
                     KIEEKTVTWFESGKAVRKFYVMASALIHRALEQIDEQTVIVIPGGRAALAAYKQERDP
                     SLKERLKKHKLAKYRLLRSVLDLPILTRETFEELIASDPVEKAENQLMMF"
     gene            complement(2201..>3092)
                     /locus_tag="PLT08_10605"
     CDS             complement(2201..>3092)
                     /locus_tag="PLT08_10605"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HNE04959.1"
                     /translation="GIPTFIRHIIVVDDASPDSSADLVTAAAKKNRRILLVRHAKNQG
                     VGGAMVTGFKKALELGAEIVIKLDGDGQMDPQYIPALITPLITGEADYAKGNRFRDFD
                     ALRQMPIVRRIGNLGLSFLTKAATGYWNIFDPTNGYFAIRAEMLAQLSLDRIDKGYYF
                     ETSMLSRLYLRDAFVQDVTIPARYRNEVSSLSIRRVLFEFPYKLTRTLIKRIILKYFI
                     FDFSMMSIYLLTGIPLLLFGLIFGITKWIQYAELGIAAPTGTVILPTLSVILAIQILL
                     SAIEIDLNAAPRKAISKALV"
BASE COUNT          788 a          778 c          737 g          789 t
ORIGIN      
        1 ttagaacatc atcaattgat tttcggcttt ttccaccggg tcactggcga ttagttcttc
       61 aaaagtttcg cgagtcagaa taggcaggtc taacacactg cgcaatagac gatattttgc
      121 caatttatgt ttcttcaatc gttccttgag tgaagggtcg cgttcttgtt tataggctgc
      181 caacgctgcc cgcccgccag ggataacgat gacggtctgt tcatcaattt gctccaacgc
      241 acggtggatc aatgccgaag ccattacata aaatttacga acagcttttc cactttcaaa
      301 ccaagtgact gttttttctt caattttagt tttgtattcc aggcgtttcc caatttcttc
      361 caacaatgcg aaaactgttt tcatttcatc acggcgagct gacgcataat cttcattgcg
      421 caaattccaa attccgccgt ctttctctgc ataggaattc aggatggcat agatcaaacc
      481 tttggatgga gtcatcaagc ctgtgaactg cttattcaat tcttcttcta cttctaaaaa
      541 gatcgtttgc ggattttttt gcagatagtt cacaatgcca acttcgatct tgtctgaaag
      601 cgattcagca tgtccgcttg ccaagctcca tacaccagta tcaacgcctt cgcccgtaga
      661 gtaatgaaca aattcatggc tatctttcag ggcttgttcc atcacacttt gagtcttgcg
      721 catggcaaag tcaaattcat cattggcttg cttcaatgca ttgacctccg ccaacgcgat
      781 cagccctgcg gtatgcaaat gcaaataccc cgccggttca ccacgtgctg ctatatagct
      841 acgcattgca ttacgtgccg tttctacttc tatttcagca gatgctgact ttttgccgct
      901 tttccataac acttgcacgg ggtcatgttc agtgcgcaat gcgacacttt ccaatttgaa
      961 ccccgccgcc gatgccgcgg ttaaagccga agtcataaaa ggtgcttcgg gctcaggcat
     1021 caaaccaaag aacggcacac catctgcaag caattcatcc aagtgactga acatcgcgaa
     1081 caaagctgtg gcactccatg cccagtcata ccgcctgcgt cgcaacgcta ctttatatgg
     1141 ctccaccgcg tcactgcccc acaaccagcc tgcccacaat gccgaaagag tccaaaacgc
     1201 ctgattgggt ctcggaatag aagccaccac cgccgcaata ggaatctcac gtttgacttc
     1261 atgggcaagg tctttcaagc gaccttcata gatgcagatg ccgcccgtct ctggaatttt
     1321 cttgggccac gcttcaacca caacgggtga gcctgattca gcaaacagac ggatgccacg
     1381 ctcgagcatg gtccacaaat tgttctcgcg aaattggctt ggtgtgttca gcaacttcgg
     1441 gcgcggacgt tccgccggat gcccccaaag cgtattgcca gcatcgaaag ccagtaacaa
     1501 caaggcggtc aacgcacggc gacgtgactc gttcaaatcc aaactatcca accgattcaa
     1561 aatggtaccc aacacataca acggacgcgg caaataatgc tcaatggctt cttccgcata
     1621 gacacggtct tcatctttga gcgaaaccac acgctcgaag agacgtgtgc ggtgtagagt
     1681 atctgtccca gcaatttgca gcgcgatgtc tttatctgag tctgtagtta ttcgttcacc
     1741 agagtctttg caatgtgggc attcatacag ttttgcatag ggcacatcct cgcctttgcg
     1801 ccagaggaaa gcctgtgcat aaatctgttt ctcgcatttt tcacattgcg taaggtaaag
     1861 ggattgtaaa tgttgttcaa gtcgttcgtc cccttttttc acggcgccaa gatcggcaag
     1921 cgcagcgaca aagtccgctt gcggtggcgg gttggcaaag acttccagca aaaagcgtgt
     1981 gatgggatta tttgccgtga cgagcacacg gtatccgctg cgggcggctt cgagcacaag
     2041 cttcggggag aatccgaatg ggtcgagcaa ccagtttccg gctagagtct gacccgagag
     2101 ccagcctgaa atggtgccat cttccagcgc aggtaaaaag cgcgagagtg gaccaacgtc
     2161 tacgggtttg aatccgggaa tgtaaggttg agattccata tcacaccagc gctttgctga
     2221 tggctttgcg cggagcggcg ttgaggtcaa tttcgatggc agaaagcaaa atttgaatgg
     2281 cgagaatcac cgagagcgtt ggcaaaatga ccgtgcctgt gggcgcagcg attcctaact
     2341 cggcatattg aatccatttg gtgatgccga agatcaaccc gaaaagcaat agcggaatgc
     2401 cggttagtaa atagatcgac atcatcgaaa aatcaaagat gaagtatttg aggatgatgc
     2461 gtttgatgag agtgcgggtg agcttatatg gaaattcaaa cagcacacgc ctgatcgaaa
     2521 ggcttgaaac ttcatttcgg tagcgtgcag gaatggtcac atcttgcaca aaggcatcgc
     2581 gcaggtataa gcgcgagagc atagacgttt caaagtaata gcctttatca atgcggtcga
     2641 gcgagagttg cgccagcatt tcagcacgaa tggcgaagta cccattggtg gggtcaaaaa
     2701 tattccaata ccccgtggcg gctttggtga gaaagctcaa gccaaggttg ccgattcgcc
     2761 gtacgattgg catttgcctt aatgcatcaa aatcgcgaaa gcgattgcct ttggcatagt
     2821 ctgcttcacc ggtaatgagt ggcgtaatca gcgcagggat gtattgtggg tccatttgcc
     2881 catcgccatc gagttttatt acaatttcgg caccaagttc taaggctttc ttaaaacctg
     2941 ttaccatcgc gccacctaca ccttgatttt ttgcgtgacg gacgaggagg atgcgtcgat
     3001 tctttttggc ggcagcagtg accaggtccg cgctggaatc gggcgaagca tcatccacta
     3061 caataatgtg gcgaatgaaa gtaggaattc cc
//
LOCUS       DAOQJE010000391         3091 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_21023_length_3091_cov_6.685112, whole genome shotgun sequence.
ACCESSION   DAOQJE010000391 DAOQJE010000000
VERSION     DAOQJE010000391.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3091)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3091)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3091
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21023_length_3091_cov_6.685112"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            112..465
                     /gene="ndhC"
                     /locus_tag="PLT08_10610"
     CDS             112..465
                     /gene="ndhC"
                     /locus_tag="PLT08_10610"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit A"
                     /protein_id="HNE04960.1"
                     /translation="MNEWLYVALFIVVGLIIPIGAIAAGFIFGPKKPNPIKESTYECG
                     IEPVGEAWIQFKAQYYIFALVFLVFDVEAVFLFPWAVKLGQLGLYAVIEGIIFVTILV
                     VGLVYTWRKGMLEWA"
     gene            540..1736
                     /locus_tag="PLT08_10615"
     CDS             540..1736
                     /locus_tag="PLT08_10615"
                     /inference="COORDINATES: protein motif:HMM:NF012373.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit H"
                     /protein_id="HNE04961.1"
                     /translation="MSFWNDPLRVAAEWLEGILTGWGLNAIAAHVLVVFLGVFLLITL
                     LMVLDIFLVWIERKVVARFQDRLGPNRLGPFGIIQPFADIIKLIIKEDTTPSDADKVV
                     YNLAPILSMMSVLILWAVVPLAPVMLGTDLNVGVLFLIAAGAIGTLSIIMAGWASNNK
                     FALIGGFRQVAVMVSFEVPMLTMLLIPVIFSGSMNMNAIIQAQDPIPFWVLSPLGALI
                     FLIAAIAELGRAPFDLAEGESELVSGFNIEYSGMKFGMFYAGELLHAFTFGGFWAILF
                     FGGYRFFGLEQVSPFLAILILIIKAMLGYWVIMWVKYTMMRIRIDQMLSFNWKFLTPL
                     SFALLLVTALMNALLASASTWLYVLGMILANIVTGWIALEIARSATRSERERIEGVKQ
                     PVEAHH"
     gene            1797..2318
                     /locus_tag="PLT08_10620"
     CDS             1797..2318
                     /locus_tag="PLT08_10620"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF012709.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit J"
                     /protein_id="HNE04962.1"
                     /translation="MTAQQIIFLIVALYTLISATMVVTSRNLVHAALWLVSTLFGVAV
                     VYALLNANFIAVVQVVVYIGAIAILFIFAVMLTRKDMRDTGPQMNKSWWAGALVAVLT
                     FAGLVFMLSGWTGFTKTAIELPAGFDGINLLGEALVSPNAFVLPFEVASVLLLAALVG
                     AVYVAFNPRSDKK"
     gene            2334..2645
                     /gene="nuoK"
                     /locus_tag="PLT08_10625"
     CDS             2334..2645
                     /gene="nuoK"
                     /locus_tag="PLT08_10625"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoK"
                     /protein_id="HNE04963.1"
                     /translation="MVPLSWYLFLSAALFSIGLFGVLVRRNAIAILLGIELMLNAVNI
                     NLVSFWRYSDALTSMNGQVFSIIVFAVAAAEVSVGLALVISVYRRRNTVVADELDMLK
                     W"
     gene            2652..>3091
                     /locus_tag="PLT08_10630"
     CDS             2652..>3091
                     /locus_tag="PLT08_10630"
                     /inference="COORDINATES: protein motif:HMM:NF012868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE04964.1"
                     /translation="MTHELVTQLIWFIPLPPALAFALIVLFTNKSKALSHTVAIIAAF
                     LSWLGSMIVFFNAVKIEHFAEHPFAAAIDWLPTGETYLKIGVLIDPLSATVLFFVAWC
                     VLMIFIYSVGYHNFGQPAGDHDHKGLPPHGATVHGHTVPSVEPM"
BASE COUNT          620 a          706 c          790 g          975 t
ORIGIN      
        1 gaaaaagtga caaatatcac ccaaaaaatt gacaaccatt aatggggggc taatttgttc
       61 tttatggtac aatgcgcaag ttgtaataca tttcccaatg gagcgaatta aatgaatgag
      121 tggttgtatg tcgccctgtt tattgttgtg ggtctgatca tcccaattgg tgcgatcgcc
      181 gctggcttta tttttggtcc taagaagccg aacccaatca aagaatccac atacgaatgc
      241 ggtattgaac ctgtaggtga agcctggatt cagttcaagg cgcaatacta tatttttgca
      301 ttggtctttt tggtgttcga tgtagaagcg gttttccttt tcccttgggc ggtgaaactc
      361 ggtcaactcg gtttgtatgc tgtgatcgaa ggtatcatct ttgtcaccat tttggtcgtt
      421 ggtttggtgt acacatggcg caagggcatg ttggagtggg cgtaaataca cggtaaatta
      481 aagagaaggc tgatgggatt tttcctctca gccttttctt tgtgtaacag gagtgattta
      541 tgagtttctg gaatgacccc ctgagagttg ctgccgagtg gcttgagggc atattgaccg
      601 gctggggctt gaatgccatt gccgcacatg tgcttgtggt tttcctcggt gtattcctgc
      661 tcatcacatt gttgatggtg ttggatatct tcttggtctg gatcgagcgt aaagtggtgg
      721 ctcgcttcca agaccgcctt ggacccaatc gcttgggtcc gttcggcatt attcagcctt
      781 ttgccgatat tatcaaactc atcatcaaag aggacacaac ccctagtgat gcggacaagg
      841 ttgtttacaa ccttgctcca attctctcga tgatgtcagt tttgatctta tgggcagtgg
      901 ttccgctggc acccgtaatg cttgggacag acctgaatgt aggcgtgctc ttcctgattg
      961 cggctggtgc tattggaaca ctctctatca tcatggcggg gtgggcatca aataacaaat
     1021 tcgccttgat cggtggtttc cgtcaggtgg cggtgatggt ctcatttgaa gttccgatgt
     1081 tgaccatgct attgatccca gtaatatttt cgggatcgat gaacatgaat gccattattc
     1141 aagcacaaga ccctattcca ttttgggttt tgtctccgct tggggcgttg atcttcctga
     1201 ttgctgccat tgccgaactg ggacgcgctc ctttcgacct tgctgaaggt gaatccgaat
     1261 tggtctctgg cttcaacatc gaatactcag gtatgaagtt cggtatgttc tatgctggtg
     1321 aacttctgca tgcctttacc tttggtggtt tctgggcaat ccttttcttt ggcgggtatc
     1381 gcttcttcgg tttggaacag gtcagcccct tccttgccat ccttatcctc atcatcaaag
     1441 ccatgctggg atattgggtc atcatgtggg ttaagtacac catgatgcgt atccgtatcg
     1501 accagatgct ttcctttaat tggaaattcc ttacgccctt atccttcgcc ttgttactgg
     1561 taactgcgtt gatgaatgcg cttcttgcgt ccgcttctac ttggctctac gtcctcggca
     1621 tgatccttgc gaatatagtt accggctgga tcgcgcttga gatcgcacgc tctgccaccc
     1681 gtagcgaacg cgagcgcatc gaaggcgtaa aacaacctgt ggaggctcat cactaggaga
     1741 cggcatttta caaattacgt actccgtgat ttgtaaagac cggaaatgga tattctatga
     1801 cagcacaaca gattattttc ctcattgtgg ctctttacac cctcatctct gcgacgatgg
     1861 tggtgaccag ccgcaacctt gttcacgctg cattgtggct tgtctccaca ttatttggtg
     1921 tggcggtggt ctatgcgctt ctgaatgcga acttcatcgc cgtggtacag gttgtcgttt
     1981 acatcggcgc catcgccatt cttttcatct tcgctgtgat gcttactcgt aaagatatgc
     2041 gcgatacggg tccgcagatg aataagagtt ggtgggcggg cgctttggtg gctgtactca
     2101 cctttgccgg gttggtattc atgctttcgg ggtggaccgg tttcacgaag acggcaatcg
     2161 aactcccggc agggtttgac gggatcaatc tactcggtga agcgttggtt tcgccgaatg
     2221 cttttgtttt gccgttcgaa gtggcatccg ttctgttatt ggcggcgttg gtgggcgcgg
     2281 tgtatgtcgc gttcaaccct cgttcggata agaaatagga ggcgcacaca attatggttc
     2341 ctctttcctg gtatttattc ctttcggcgg cattgtttag cattggcttg tttggtgtgc
     2401 tggtgcgacg caatgcaatc gccatccttc tcggtattga gttgatgctc aatgctgtga
     2461 acatcaacct cgtttctttc tggcgctata gcgatgcctt gacctcgatg aatggacaag
     2521 tcttttccat tatcgtcttt gcagttgcgg cggcagaggt ttcggtgggc ttggctttgg
     2581 ttatttctgt ctatcgccgt cgcaatacgg tggttgcaga tgaactcgat atgttgaagt
     2641 ggtagggacg catgactcat gaattagtga cacaattaat ttggtttatt cccttgccac
     2701 ccgctcttgc gtttgcgctg attgttctgt tcaccaataa aagcaaagcg ctgagccaca
     2761 ccgtggcaat cattgctgca ttcctttcat ggttgggttc catgattgtt ttcttcaatg
     2821 cagtgaagat cgaacatttt gccgagcatc catttgctgc tgccattgat tggctgccga
     2881 ccggcgagac ctatttgaag atcggtgtgt tgatcgaccc gctctctgcc acagttttat
     2941 tctttgtggc atggtgtgta ctgatgatct tcatctacag tgttggttat cacaacttcg
     3001 gtcaacccgc tggcgaccat gaccataagg gtcttccgcc tcatggcgcg accgttcatg
     3061 gtcacaccgt tccttctgtg gaaccgatgt a
//
LOCUS       DAOQJE010000392         3084 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_21109_length_3084_cov_5.367118, whole genome shotgun sequence.
ACCESSION   DAOQJE010000392 DAOQJE010000000
VERSION     DAOQJE010000392.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3084)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3084)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3084
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21109_length_3084_cov_5.367118"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..511)
                     /locus_tag="PLT08_10635"
     CDS             complement(<1..511)
                     /locus_tag="PLT08_10635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE04965.1"
                     /translation="MIPARLSPRLMNRFVETLSKEVGADTYHAVLSNSGLSRDWQKPE
                     FFLAFDNKQTAEAYAKLQSALRTYYGRGARGILLRIGSKLWEPMLNDASFASKAQATL
                     IRGFPKPLKRKPALELLASTLGAGRGDVTVHTLDLDLLLVDHSSPTTLGQKENSPVCF
                     VTLGLIRECL"
     gene            complement(511..1374)
                     /locus_tag="PLT08_10640"
     CDS             complement(511..1374)
                     /locus_tag="PLT08_10640"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF025054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNE04966.1"
                     /translation="MINLHLHIRRAVAQDHRQLSNLLFQESNTHRHLDWRTALEWIGV
                     HNFWVLEDSGTVVAALACPEDPPDIAWIRLFSFSPHLSGPEAWSDLWNFASAEIRAIN
                     PRAEAASIVMKQWFQTLLLDSGFEPRLSIVLLELKSEDYVPVRISDAIRMRPMQEADL
                     GIVEDVDREAFGKFWHNTSDALQRAHGQSFYSSVAEDANGIIGYQISTGNPFGVHLAR
                     LGVRPEAQGKGVGSALVHDLIQRAGGLQSGRLSVNTQDDNVASLRLYSKLGFLRTGEH
                     YPVFIHSLGAS"
     gene            complement(1371..1892)
                     /locus_tag="PLT08_10645"
     CDS             complement(1371..1892)
                     /locus_tag="PLT08_10645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE04967.1"
                     /translation="MSLEPHQLDAKGKQAFAKKNFVEAAEYFRQAAEGYTQGRAGLEA
                     AESRNNLSVALLQAGKPQEALDAALDTDKVFEGAKDVKRQAMAFGNQAAALEGLHRYD
                     EAIQKYELSAELFGSIDEGDLRAMVMKSAAAIKLKTGKITDSAFKMMGSLDVKKNPTF
                     FERIMKFLMKFIR"
     gene            complement(1898..2572)
                     /locus_tag="PLT08_10650"
     CDS             complement(1898..2572)
                     /locus_tag="PLT08_10650"
                     /inference="COORDINATES: protein motif:HMM:NF024949.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HNE04968.1"
                     /translation="MTNAQITIREKKLGLLIRDARVAERRSIKECADAIGVKAGVFRA
                     YEEGRRAPSLPELEALVFFLKIPISQFWGNETISDAPEPLKPEDITKLIALRHRMIGA
                     LIRQERTNANMSIRHLSSETGIPQSRLKAYELGERTVSVPELESILAVMGSRIENFFD
                     QSGPVGEWMTTQLAMQKFMDLPNEFQEFVCQPVNRPYLELAMKLSSMSRDKLRSVAEG
                     LLDITL"
     gene            complement(2612..>3084)
                     /locus_tag="PLT08_10655"
     CDS             complement(2612..>3084)
                     /locus_tag="PLT08_10655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HNE04969.1"
                     /translation="RIVVGEVRGGEALDMLQAMNTGHDGSLTTVHSNSPRDAISRLET
                     LVLMAGMELPLKVVRTQISSAIDLIVQQTRLKDGKRKVTAITEVAGMEGDVVVLTDIF
                     KFNQTGVTQDGQVLGELQPTGIRPNFTPRLEAAGFKLGAEVFMTSKQQQQSSRR"
BASE COUNT          842 a          746 c          722 g          774 t
ORIGIN      
        1 ataaacattc acggatcaag cccagggtta caaaacacac aggggaattt tctttttgtc
       61 ccaacgtggt aggagaagaa tggtcaacca gaagcaggtc aagatccaaa gtatgaaccg
      121 tgacatcgcc gcgtcctgca ccaagggttg aagcaaggag ttcaagcgca ggtttacgct
      181 tcaatggttt gggaaacccg cgtatgagag ttgcctgagc tttagaggca aaagaagcat
      241 cgttcaacat gggttcccaa agcttagaac cgattctcaa aaggataccg cgtgcgccgc
      301 gcccataata ggtgcgcaaa gctgattgca atttggcata tgcttctgca gtttgtttat
      361 tatcgaaggc aagaaaaaat tctggcttct gccaatctcg tgacaaacca gaatttgata
      421 aaactgcatg ataggtatcg gcgcccactt cttttgaaag agtttctacg aagcgattca
      481 ttaggcgtgg agaaagtcgg gcaggtatca ctatgacgct cccaaggaat ggataaaaac
      541 agggtagtgc tctcctgtgc gaagaaagcc gagtttcgaa tacaaacgca atgacgcgac
      601 attatcatcc tgcgtattga cagatagtct accagactga agcccgcctg ctctttggat
      661 caaatcatgt acgagggctg agccaactcc cttgccttgc gcctcagggc ggaccccaag
      721 ccttgcaagg tgcaccccaa acggattccc cgtactaatc tgatatccaa ttattccatt
      781 ggcatcttca gcgacagagg agtagaaaga ttgaccatgt gcacgttgga gagcatctga
      841 agtattgtgc cagaattttc caaaggcttc acggtctacg tcttcaacaa taccaaggtc
      901 tgcctcttgc atcggtcgca ttcgaatagc atcactgatt cgaacaggaa cataatcttc
      961 acttttgagt tcaagaagaa caatacttaa gcgtggttca aacccactat ctaaaagcag
     1021 cgtttgaaac cactgtttca ttacaatcga agctgcttca gcacgcgggt tgatagcccg
     1081 aatctcagcg gaggcaaagt tccataggtc agaccaagct tcgggtccag aaagatgcgg
     1141 gctaaatgaa aagaggcgaa tccatgcgat atctggcggg tcttcagggc aagccaaggc
     1201 agccaccaca gtccccgaat cttccaatac ccaaaaatta tggacgccaa tccactcaag
     1261 ggctgtgcgc caatcgagat ggcggtgtgt atttgattct tgaaatagga gattggaaag
     1321 ctgacgatgg tcctgcgcaa cggcgcgccg gatatgcagg tgaagattga tcatctgata
     1381 aatttcatga gaaacttcat gatacgttca aaaaaggttg gattcttttt tacgtcgagc
     1441 gagcccatca ttttgaaagc agaatcggtg atcttgcctg ttttgagttt gatggcagcc
     1501 gcagatttca tgaccattgc gcgcaggtca ccttcgtcaa tactaccaaa caactctgca
     1561 gagagttcgt atttttgaat cgcttcatca tatcgatgaa gaccttcgag cgccgcagct
     1621 tggttaccaa acgccatggc ttgacgtttc acatctttgg caccttcaaa gactttatcg
     1681 gtatcaagag ctgcatcgag tgcttcctga ggtttgcctg cttgcaagag tgcaacactt
     1741 aagttgttac gagactctgc tgcttcaagc ccggcacgtc cttgggtgta cccttcagct
     1801 gcctggcgaa aatattcagc agcttcaaca aagttctttt tggcaaaggc ttgtttccct
     1861 ttggcgtcga gttgatgagg ttcaagagac ataattatta aagggtgatg tcgagcaagc
     1921 cttctgcaac agagcgaagt ttatcgcgag acatgctgct aagcttcatg gcgagttcaa
     1981 gatacgggcg gttcacaggc tggcaaacaa attcttgaaa ttcattgggg aggtccataa
     2041 atttttgcat ggcaagctga gttgtcatcc actcgcccac aggaccgctt tggtcgaaga
     2101 aattttctat acggctgccc ataacagcaa gaatgctttc cagctcgggc acagaaaccg
     2161 tacgttcgcc caattcatat gccttcaatc gtgactgtgg aattccagtc tcggatgaaa
     2221 gatgacgaat ggacatattc gcattcgtgc gttcctgacg aatcagcgcg ccgatcatgc
     2281 gatggcgcaa agcaataagt ttggtaatat cttcaggttt taagggctcg ggagcatctg
     2341 aaatagtttc attgccccaa aattgcgaaa tgggaatctt taaaaagaat acaagagctt
     2401 ccagttcagg caaagagggt gcgcgccgtc cttcttcata ggcacggaac acacctgctt
     2461 ttacgccaat cgcatcggcg cactccttga tactgcgacg ttctgccaca cgggcatctc
     2521 gaatcaacaa accaagtttc ttttcgcgaa tagtaatttg agcgttggtc atgttacctc
     2581 atcaaataag cccttattat agcagaatgc gtcaacgtct tgacgactgt tgctgttgtt
     2641 tagacgtcat aaacacctct gcacccaact tgaaaccagc cgcctctaac cttggggtga
     2701 agttggggcg aatacccgta ggctgtaact cgcccaaaac ttgaccgtct tgcgttacac
     2761 cggtttggtt aaatttgaaa atatcagtta gaacgacaac atcgccttcc ataccagcga
     2821 cttcagtgat tgctgttacc ttacgtttgc cgtccttaag acgcgtctgc tgaacaataa
     2881 ggtcaattgc cgatgaaatc tgagtgcgaa cgaccttaag cggcaattcc atacccgcca
     2941 ttaaaaccag tgtctctaga cgggaaattg catcacgcgg gctattcgaa tggacagtgg
     3001 tcaatgaacc atcgtgcccc gtgttcatag cctgaagcat atcaagcgct tccccaccac
     3061 ggacttcccc cactacgata cggt
//
LOCUS       DAOQJE010000393         3074 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_21236_length_3074_cov_4.833389, whole genome shotgun sequence.
ACCESSION   DAOQJE010000393 DAOQJE010000000
VERSION     DAOQJE010000393.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3074)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3074)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3074
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21236_length_3074_cov_4.833389"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(102..2777)
                     /locus_tag="PLT08_10660"
     CDS             complement(102..2777)
                     /locus_tag="PLT08_10660"
                     /inference="COORDINATES: protein motif:HMM:NF024627.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HNE04970.1"
                     /translation="MKRNSHLFLLITALIMALMAGAVGPSVAYADDGDGATEPSGETP
                     VTTEEEAPVFIPEVLEQAPEGTEIIVLNEEGEVLPLATQEASEVLTSENSDPMWCPEA
                     ATPGDVSCTAGFATFQELIDALTADAISGTPIYTGNGYIWVADGYNGNDNAQIEFDGS
                     VLTNIGSNNLTIQGGWSGNNDTNITGISNLDVSLVFANWGGNISLNDLAISANDGNGF
                     GLFVNNTGNVSLNNVSVSNTTANPSGFGDGAVIVTTGNVDITDSEFDDNAGNGLQVAS
                     AGTITLDTVSASNNTLTGAYLDNCLYNNTTGLCAGNGLVTITSATGNVFNGNGFIGLS
                     IDSGGGVDIDNTQANNNGLDGSVITSVDDNGTGNVSIVDSEFSGNTNAYGLDVYTDGN
                     LSLNNVIANSNGLGTFLDTTAGIGSVSVTDSIFGDSAVTGNDWSGLHIESGGTATLLN
                     VVASYNGANGTYIEAQGDIEVTNSTFNDNVHFNFPQDPGLYAKSYGGNITLTDVTADG
                     NDFGAGVVLYTKDNGVISVFNTAWGAGHFNNNGTFGIQATTEDGDIELTNVEGSNNVS
                     KGAYITSYGLGNVSVTNSEFSSNGSYGALLYSNQGGVFVTDSTFDLNTNSGLVAVSGT
                     GIELVNVTADGNGGDGVEVYSSYTWGCRGANDAPVNIVVNVDGGTFTNNTDYGIFAKP
                     GPQGDMVFVNPATFGGNGLGDYLIDLSDPEICPPQEEEEQDNPNVVPAPSNPPVEQDC
                     VNFTSTILELENGTWVKVGCPYEGFSNLDSILEGQLPGPLGAGVKFLNGIILSLTDAD
                     GNTILNQDGTVTVNLLIPEGSRGRTHSVLFWDETLNDGKGGWMKLPPYEAGTSFPLHP
                     ENPDDPRLVVSGVQQVDNMVTFTINFSGTFILTTP"
BASE COUNT          852 a          750 c          640 g          832 t
ORIGIN      
        1 tgggcttttg gcatttcaaa gttatcaaat gctgcaagcc tcaggaaggt attaagcaca
       61 aaaggacggc tctgttttga gccgtccttt tgtttgtgtc cttatggggt tgtaagaata
      121 aaagtaccag agaagttgat ggtaaaggtc accatattat ctacttgctg aacacctgaa
      181 acaacaagtc gcggatcatc tgggttttcc gggtggagcg ggaagctggt gcccgcttca
      241 taaggcggca acttcatcca accgcccttg ccatcattca aagtctcgtc ccagaacaaa
      301 acagagtgag ttctaccgcg tgaaccttcg gggatcaaga ggttgaccgt tactgtccca
      361 tcttggttga ggatcgtgtt cccgtctgca tctgttaggc tgagaatgat tccatttagg
      421 aacttcactc ccgccccaag cggaccaggt aattgacctt caagaatgct atctaaattg
      481 ctaaagcctt cataggggca accgacctta acccaggttc cattttcaag ttccagaatt
      541 gtgctggtga agttgacgca atcctgttcc acaggcgggt tggaaggtgc aggaacaaca
      601 ttggggttat cttgttcctc ttcttcttgt ggcgggcaga tttctgggtc gcttaagtct
      661 atcaggtaat cacccaagcc atttccacca aatgtagctg ggtttacaaa gaccatatca
      721 ccttgcggac cgggtttggc aaagatgcca tagtcagtgt tattagtaaa agtgccacca
      781 tcaacattaa ccacgatgtt gactggagca tcatttgcac cacggcaacc ccaagtatag
      841 gagctataca cttcaacccc atccccaccg ttaccatccg cagtcacatt gaccagttca
      901 atgcctgtgc cgctgactgc caccaaacca gaattcgtat tcaaatcaaa tgtactgtca
      961 gtgacaaaaa cgccgccctg attggaataa agaagcgctc cataacttcc attagaagag
     1021 aactcactat tcgttacgga gacgttgcct aatccgtaag aggtaatgta agcacctttg
     1081 gaaacattat tagaaccttc aacattggtt aattcaatgt cgccatcttc tgtagtagcc
     1141 tgaataccaa atgtgccatt gttattgaaa tgtcccgcac cccatgcggt attgaagact
     1201 gagattacac cattatcttt tgtgtaaagt acaacgcctg cgccaaagtc attcccatct
     1261 gcagtgacat cagtcaaggt gatgttgcca ccataactct tggcatacaa gccagggtct
     1321 tgcgggaagt taaagtggac attatcattg aatgtgctat tcgttacctc aatatcacct
     1381 tgagcctcta tgtatgtacc gttcgcgcca ttataagaag ccaccacatt caacaatgtc
     1441 gctgtgccac cggattcgat atgtaaacca ctccaatcat tcccggttac tgcactatca
     1501 ccgaatatac tatcggtgac tgatactgaa ccaattccag ccgtggtatc taagaacgtt
     1561 cccaacccat tggaattagc gattacatta ttcaagctca agttgccatc agtgtaaaca
     1621 tccaaaccat aggcattcgt gttaccagaa aattcgctat ccacaatgga cacatttcct
     1681 gtgccattat catccacact ggtaatgaca gagccatcca agccattgtt atttgcttgt
     1741 gtgttatcga tatctacgcc accacctgag tcaatgctta aaccgataaa gccatttcca
     1801 ttgaacacat tgccagtagc gctggtaatg gtcaccaagc cgtttccggc gcaaagccca
     1861 gttgtattgt tataaaggca attatcgagg taagcccccg tcagcgtgtt gttacttgca
     1921 gaaacggtat ccaatgtaat ggtcccggct gaggcgactt ggagtccgtt cccggcattg
     1981 tcatcaaatt cactgtctgt aatatccaca tttccggtag tgacaatcac agctccatcc
     2041 ccaaaaccag atgggtttgc agtggtattt gaaacagaaa cattattaag cgacacattg
     2101 ccagtgttat tcacaaaaag accaaaccca tttccgtcat ttgcagaaat tgcaaggtca
     2161 ttcaagctaa tgtttccgcc ccaatttgca aaaactaacg agacatctaa attggaaata
     2221 cctgtgatat tcgtatcgtt gtttccgctc caaccacctt gaatggtcag gttattggaa
     2281 ccaatattgg taagaacaga accatcaaat tcgatctgag cattatcgtt gccattgtaa
     2341 ccatctgcaa cccaaatgta tccattgcct gtataaatgg gcgttccaga aatcgcatca
     2401 gcggttaaag catcaatcaa ttcctgaaag gttgcaaaac cagcagtaca agaaacatca
     2461 ccgggtgtgg cggcttctgg gcaccacatg gggtcactat tctcagaagt aaggacttcg
     2521 ctagcctctt gtgtagcaag cggcagaact tcgccctctt cattgagaac aataatctct
     2581 gtcccttcgg gggcttgttc aagcacctcg gggatgaaaa ccggcgcctc ttcctctgtt
     2641 gtaactggcg tctctccaga aggctcggtg gcgccgtctc catcatctgc atatgccaca
     2701 gacggaccca ctgccccagc catcaatgcc atgattaagg cggttattaa taaaaatagg
     2761 tgagaatttc gcttcatacc atctccaatg ttggtttcta agatgtgttt ccccaaaacg
     2821 ggatgctcaa attttagaat gcacttggag atgcgtcaat taaacgtgta atgtttggct
     2881 aacatttctg ttagtgcggt atttgtgtca tggaatatga ccagattatt gaatttcaca
     2941 tttaaacaaa atcgcctctg atttctcaga ggcaattttg tttaagaaaa aggaatagcc
     3001 ctaccctgcc atacgaattg ccagcattgt caaatcatcg gcaaggggtt cggtatcgac
     3061 aaaatcattg aggc
//
LOCUS       DAOQJE010000394         3073 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_21246_length_3073_cov_5.550364, whole genome shotgun sequence.
ACCESSION   DAOQJE010000394 DAOQJE010000000
VERSION     DAOQJE010000394.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3073)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3073)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3073
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21246_length_3073_cov_5.550364"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(194..964)
                     /locus_tag="PLT08_10665"
     CDS             complement(194..964)
                     /locus_tag="PLT08_10665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE04971.1"
                     /translation="MKYVLDVNMCRNNQLVNLLKEKNEILITSDFLVEVFKANNPKFM
                     FVNNTRIIKEYPSQIFVTHDRGELVRLEISKKSPLIPDEIIDLNSTNNFRNWLINENE
                     IDLILQLVYLEAKRQVEYQKDFMNTYMYQATKKLQGLLKFENTRKQYLTDNAKLLNDI
                     KEVSITVMEGFLEKKNIINVENFKRVNSVIYSQNYVLLWRIAKWSLLNGIESVSLDKL
                     THDGFDIKYIFISSFFDGLLTKETWLKECRFDLINSIG"
     gene            complement(1223..1696)
                     /locus_tag="PLT08_10670"
     CDS             complement(1223..1696)
                     /locus_tag="PLT08_10670"
                     /inference="COORDINATES: protein motif:HMM:NF018738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation protein"
                     /protein_id="HNE04972.1"
                     /translation="MGLFSKLKKGLGIGTAKVELVDVPTQINRNTQEVSGKVTITAKS
                     DQKVLSVRVAVVEISNEEFNGQQREMKKDLAFAKLDGFDLKTDETRTLPFTIALVAGD
                     KVSVNIFGGTLEISGGPSLSSSNSIFSPVKYQIVAVADCEGVAIDPTDAKPLTIL"
     gene            complement(1864..2877)
                     /locus_tag="PLT08_10675"
     CDS             complement(1864..2877)
                     /locus_tag="PLT08_10675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HNE04973.1"
                     /translation="MTVKQVLITGACGEIGQALVQELAKKGGYRIVTSDMAPLPDSIK
                     NLVAEHVQGDLVYKVKVLYDYDFDVIFHLAASLSSKAEIATEEAHRINVEGTMQLLML
                     AAYRSEKYGKSVKFLFPSSIAAYGMPTLEAKQGAGAVKEEDWDYPHTMYGCNKLYCEK
                     LGMYYSKFHGQKHLDDNPPIMLDFRAIRFPGLISAFTLPSGGTSDYGPEMLHAAAQGK
                     DYACFVRPDTKISFMAMPDAIKSLLMMLDAPREKLTTNVYNVAAFAITAEDFRQRALK
                     AFPQANITFAPNPRRQGIVDSWPEDVDDTRARTDWNWKPDYDVDRFFEEYFLPEIRKR
                     YGK"
BASE COUNT          889 a          680 c          531 g          973 t
ORIGIN      
        1 gattcttacc attcaacttg aaggtaggaa ttccatagcc aatcgtctct tctgccgctg
       61 gcgcagattt gtggatcgtt gcacgaatct tttgcatgat ggtttggact tcgggtggat
      121 agtttgaaat aaataggtca atggggttgg taggcattta cagtttcctt tccgtgttaa
      181 tttcttctat taattatcca atactgttaa taagatcaaa ccgacactct tttaaccaag
      241 tctctttagt caataaacca tcaaaaaaag aagatatgaa aatatactta atatcaaagc
      301 catcatgtgt aagcttatct aaactaacag attctattcc attcagcaat gaccattttg
      361 ctattctcca aagcaaaaca taattttgtg agtaaattac agaatttact ctcttaaaat
      421 tttcaacatt tatgatattc ttcttttcaa gaaatccttc cataacagtg attgatactt
      481 ccttaatatc atttaataat tttgcattat cggtaagata ttgttttcga gtgttttcaa
      541 acttcaataa gccttgaagt tttttagtag cttgatacat gtatgtattc ataaagtctt
      601 tttgatactc gacctgtctt tttgcttcaa gataaaccag ttgtaatatc aaatcaattt
      661 cattttcatt aattaaccag tttctaaagt tatttgtaga gttcaaatca ataatttcat
      721 cagggattag aggggacttc ttgcttattt ccaatcttac aagttctcct ctatcatgtg
      781 taacaaatat ctgacttgga tactctttta taatcctagt attgttcaca aacatgaatt
      841 taggattatt agctttaaaa acctcaacta gaaaatcgct tgtaattaat atttcatttt
      901 tttctttaag aagatttact agctgattat tcctgcacat attaacatca agcacatatt
      961 tcattttcca tatctccggc attaggtatt cttcaaaaaa taattcacac ttaaattaga
     1021 tttttagttc aaatcattgc ccaaatcttt tgaagaacgc ctcaaatttc tttcagatag
     1081 ttaggtaaca ttatctgaaa ggttggaaat atacttcttc tattattcac cttttttatt
     1141 attgtaaacc acaacacatc ctcaaaataa aaatcccttc tatgatgtag aagggatttt
     1201 tctaaagata aaaatgaaac tactacaaaa tcgttagagg cttggcatcg gtggggtcaa
     1261 tggcaacgcc ttcgcaatct gcaacggcta cgatttgata cttcaccggc gagaagatgg
     1321 aattggaact ggataaactc ggtccgcctg aaatttccaa ggtgccaccg aaaatgttca
     1381 cagatacctt gtcccctgcc accaaagcga tagtaaaagg caaagtacgg gtttcgtctg
     1441 tctttaggtc aaatccatcc aactttgcga aagcaaggtc tttcttcatt tcgcgctgct
     1501 gcccgttaaa ttcttcattt gatatttcca ccactgccac gcgaacagac aaaaccttct
     1561 ggtcgctctt ggctgtaatg gtcacttttc ctgaaacttc ttgcgtgttc ctgttgatct
     1621 gagttggcac atcaaccaac tccacttttg ccgtaccaat ccctaaacct ttcttgagct
     1681 tgctaaaaag acccataatg acgttactcc tttggaaaat atatacagaa taaaaaactt
     1741 attccatagt gaacgtatga tagaggttta aaaatagaat gacaagatag aaaccattta
     1801 caatctacaa acaaaagact gtgcaaattt gcacagtctt ttgtttgtta taagcagttt
     1861 tctttacttt ccgtatcgct ttcgaatctc aggcaagaaa tactcttcaa agaaacgatc
     1921 cacatcgtaa tcgggcttcc agttccaatc ggtgcgggcg cgggtatcgt ccacgtcttc
     1981 gggccacgag tcgacgatgc cttggcggcg cggattcggt gcaaaggtaa tgttcgcttg
     2041 cgggaacgcc ttcaatgcac gctggcggaa atcttcagcc gtaatagcaa acgctgccac
     2101 gttatataca ttcgtagtca atttttcacg tggtgcgtcc aacatcatca acaacgattt
     2161 gatggcgtcg ggcattgcca taaaggaaat tttggtatcg gggcgcacga aacaggcata
     2221 gtctttaccc tgagccgccg catgtaacat ttcgggacca taatcacttg tgccaccgct
     2281 cggcaaggta aaggcgctga tcaagcccgg gaagcggatc gctcgaaagt ccagcatgat
     2341 cggtgggtta tcatccaaat gtttttgacc gtgaaatttg ctgtaataca tccccaactt
     2401 ttcgcaatac aacttattac aaccatacat cgtgtgcgga taatcccaat cttcttcttt
     2461 caccgcacct gcaccttgct ttgcttcaag cgttggcata ccatatgcgg cgatcgaact
     2521 tgggaacaag aacttgaccg acttgccata tttttctgaa cgatatgccg ccaacatcag
     2581 cagttgcatc gtcccttcca cattgatgcg atgtgcttcc tccgttgcaa tctctgcttt
     2641 tgaagaaagc gacgccgcca aatggaagat cacatcaaag tcataatcat agagaacttt
     2701 tactttataa accaaatcgc cttgcacatg ctctgccact aaatttttga tcgaatctgg
     2761 caaaggcgcc atatccgatg tcacaattcg gtaaccgccc ttcttcgcca attcttgcac
     2821 cagcgcctga ccaatttctc cacacgcacc agttatcagc acttgcttca cggtcatctt
     2881 catctcctga tatgctagaa tcatgctaat gtcaccctga gggagcgttc ttcgcgaccg
     2941 aagggtctct acttctggtg tcagagatcc tttgctgtcg ctcaggatga catcaatatg
     3001 attttacgac atattgaatt aaaactttgt cataaaaccc taatgacttt aaaacgactt
     3061 ttcttctcaa gtt
//
LOCUS       DAOQJE010000395         3070 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_21290_length_3070_cov_6.581758, whole genome shotgun sequence.
ACCESSION   DAOQJE010000395 DAOQJE010000000
VERSION     DAOQJE010000395.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3070)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3070)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3070
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21290_length_3070_cov_6.581758"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(489..1100)
                     /locus_tag="PLT08_10680"
     CDS             complement(489..1100)
                     /locus_tag="PLT08_10680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE04974.1"
                     /translation="MDTHSPSTTDSETEAKKLRRHFKFTNDDLEENRRGVLSKKQIER
                     IAYYERGGKIMFSIIGVLLLIFPIGFSKMIISETIKMYQNESLFESAVWAWGFFLAIL
                     VIFGLFLLVIGVVGIFLIVSQFLKIKPYKLVSIRGLARLEKGHGNRFSHVYYDLHINN
                     QQFDGDSTLNKVIVQGAEYIVYYLESNAEIMSIEKVSNEMSVA"
     gene            complement(1235..2014)
                     /locus_tag="PLT08_10685"
     CDS             complement(1235..2014)
                     /locus_tag="PLT08_10685"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNE04975.1"
                     /translation="MGTASVQGQIWGTRAKDWADAQEGVAIPLFEAVLHETEVGENTS
                     VLDIGCGSGIFCEMAAKRGARVSGLDASEHLVAIAHERVQSGEFRTGEMEELPYDNQT
                     FDVVTGFSSFQFAANPVNALKEASRVSKTGTVVIAVFGKPEESESTAYIKAMGALLPP
                     PPPGAPGPFALSADGALEALTIKAGLIPSTVKAVNCPWDYPDEETALRGLMSSGPAIR
                     AIQEKGEEIVRDHILQVIAPFKTRSGGYYLTNSFHYLIAKT"
     gene            complement(2364..2921)
                     /locus_tag="PLT08_10690"
     CDS             complement(2364..2921)
                     /locus_tag="PLT08_10690"
                     /inference="COORDINATES: protein motif:HMM:NF013989.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HNE04976.1"
                     /translation="MRKILVSMFMALDGVVEAPNQWSFQFGSKEQQKYKYDELFTCDA
                     LLLGRETYQGFAAAWPNMPGTGDYGVRMNSIPKYVVSTTLQEAEWNNSKIIKNNISEE
                     LTTLKQQTGQDIFIFGSSTLVNSLMQYDLIDEYRLMVFPVIVGSGKRLFQDMNSKTTL
                     KLIKTETFSSGVVVLSYQPDRSEPK"
BASE COUNT          820 a          672 c          631 g          947 t
ORIGIN      
        1 tatcggctgt tccgttagta catacatcat tgatgtagca aagttcacca tccagtatga
       61 agctcccttt tatttcgcag aggttatcat caaaagtggc gacaaaaggg gttggtattt
      121 tggtaggttg tgatatcgcg gtaagtagag ttgttggggt aggagtttgt aacaataacg
      181 gtctcgttcc gcaactcgaa attatcagac tgaccagtag gatgaaaaat gttttgatta
      241 tcaatttgat gttcatttga ccatccagtg ctgttacaag cgcatccttg ttactacttg
      301 cagcgattgt tagctatttt aattgcggtt tagattaacc gcctaaagga ttgtggctcg
      361 tccctgatag ggatgcctgc gctgggacgt agaaaaatct ttgagattgg cgcataccct
      421 ccgggcagtc ccccagacag agcaagcttt gttggacgtt tggatggaaa tatagaataa
      481 tttcattctt aagctacact catctcgttt gacacttttt caatagacat tatttctgca
      541 ttactttcta aataatatac gatgtattcg gctccctgaa caataacctt gtttagagtg
      601 ctatctccat cgaactgttg attgttgatg tgtaaatcat aatagacatg gctgaaacga
      661 ttgccatgtc ctttttcaag gcgagcgagc ccacgaatac tgactagttt atatggtttg
      721 atttttaaaa actgactaac tatcaaaaaa atacccacca cgccgattac aagtaagaat
      781 agcccaaata tcaccaagat tgcaagaaaa aatccccaag cccaaacggc actctcaaat
      841 aaagactcat tttggtacat tttaatagtt tcagagataa tcattttgct gaaaccaatg
      901 ggaaatataa gtaatagaac gccaataata ctaaacataa tttttccacc ccgctcataa
      961 taagcgattc gttctatctg ctttttactg aggactccac gccgattttc ttcaagatcg
     1021 tcatttgtga acttgaaatg tctcctcagt ttctttgctt cagtttcgct atcggttgta
     1081 cttggtgaat gagtatccat aatgaattga tctctgtgaa tgaacgggac tgaaatgccc
     1141 aacgatttgt gactcgtccc caatagggaa tacctgatcc ggcgcgcgga cttagcttgt
     1201 ccgtccagcc agaaatgata tgctgtcttt attattaagt ttttgcaatg agatagtgga
     1261 aactatttgt caaataatat ccacctgacc ttgttttaaa tggagcgata acttgcaata
     1321 tatggtcgcg tactatttct tcgccttttt cttgaatggc tcgtattgct ggacccgatg
     1381 acattaaacc gcgcagagca gtctcttcgt cagggtaatc ccacggacaa ttgacagcct
     1441 ttaccgttga agggatcaag cccgctttga tggtaagggc ttctaaggca ccatcagcag
     1501 aaagcgcaaa tggacccggc gctcctggtg gtgggggagg taataatgcc cccattgctt
     1561 taatataagc tgtcgactcg ctctcttccg gtttcccaaa tacagcgatt acaaccgttc
     1621 cagtttttga tacgcggctt gcttctttca atgcgttgac gggattggct gcgaattgaa
     1681 atgagctaaa tcctgttact acatcaaatg tctgattgtc atagggcaat tcttccattt
     1741 cccctgttcg gaattcgcca gattgtacac gctcgtgagc aatggctacg agatgctccg
     1801 atgcgtccag tccactaacc cgcgcaccgc gttttgctgc catttcacaa aaaatacctg
     1861 agccacaccc aatatctaga acggatgtgt tttcccctac ctccgtttcg tgtaaaaccg
     1921 cctcaaagag aggaatggct acaccttctt gcgcatctgc ccaatcttta gctcgtgttc
     1981 cccaaatttg tccttgtaca cttgctgttc ccataaagtt tactccttgg agaaactgat
     2041 atttaaaatc ctacggctgt tgacccttat tgctttatgg ttgctagtat accctgacag
     2101 gaaattgtgg actttgtttt cacccattct ctcaatacca tattgaagcg cggacttaca
     2161 cccatataaa tagaaacact cgggagattg agaactcccg agtgttatat tgtgcccaac
     2221 aggttatgac ctgtccctgc acgagccgta acctgttagg tgaggagatg cttatctcat
     2281 ttgagcaaag ccttccagcc agaaaaatca ggcagtatct tgttgcacgc tttgttgggc
     2341 gtttttttgt aggaagctaa tctctatttg ggttcacttc tgtcaggctg gtaggaaaga
     2401 acaacaacac ctgaactgaa ggtctctgtc ttgattagtt ttagagttgt cttactattc
     2461 atatcttgga agagtcgctt tccgcttccc actataacgg gaaaaaccat cagtctatac
     2521 tcatcaatga ggtcgtattg cattagtgaa ttgacaagag tactacttcc aaaaataaaa
     2581 atatcctgac ccgtttgctg ttttaatgtt gttagttctt cagaaatatt gttttttatt
     2641 atctttgaat tgttccattc ggcttcctga agggttgttg aaacaacata tttggggatg
     2701 ctgttcattc ttacgccata atctcctgtg ccaggcatgt ttggccaagc ggctgcaaac
     2761 ccttgatagg tttctcgccc caaaaggaga gcgtcacatg taaagagttc atcatattta
     2821 tacttctgct gttctttgct cccaaactgg aaagaccatt gatttggggc ttctacaaca
     2881 ccgtctagtg ccatgaacat ggataccaaa atttttctca ttgaattttt ccttttttat
     2941 tgataagaga taagttttgg tgatttaggc ataaaaccaa tatgtaaact tgtgccaaac
     3001 gccaaacgga ttgcggctcg tccccgatag gggatacctg cgctggggcg gggacagcga
     3061 agccgtccaa
//
LOCUS       DAOQJE010000396         3063 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_21371_length_3063_cov_5.331117, whole genome shotgun sequence.
ACCESSION   DAOQJE010000396 DAOQJE010000000
VERSION     DAOQJE010000396.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3063)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3063)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3063
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21371_length_3063_cov_5.331117"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..257)
                     /locus_tag="PLT08_10695"
     CDS             complement(<1..257)
                     /locus_tag="PLT08_10695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE04977.1"
                     /translation="MIRPYQEKDFDAVTALWFNAMLVAIPDLMKRMGYTLEGSLNYFR
                     NGILAENQLWVYELDGKPVAFLALKDDFIDRLYVAPEFHRRG"
     gene            complement(453..899)
                     /locus_tag="PLT08_10700"
     CDS             complement(453..899)
                     /locus_tag="PLT08_10700"
                     /inference="COORDINATES: protein motif:HMM:NF020157.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1761 domain-containing protein"
                     /protein_id="HNE04978.1"
                     /translation="MDFSSINWLAVLFCVLASMVIGGVWFGPKTFFPIWWKAVGKAGE
                     PSMGGGENTPGSSLGMGAVWGGVVFAAFVESVFMSMMVHAMGSISGGVTLASGALTGF
                     LIWLGFVASSSLTNKLFADRLQAWVLEQGNHLITFVVMGAIVGIMG"
     gene            complement(1055..2470)
                     /locus_tag="PLT08_10705"
     CDS             complement(1055..2470)
                     /locus_tag="PLT08_10705"
                     /inference="COORDINATES: protein motif:HMM:NF012512.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HNE04979.1"
                     /translation="MTTGLPESKEKRVFNNVLGAMGNTPLVKLERIGKDLPVPLYAKL
                     EFMNPGGSVKDRVGANIIEQAEKRGEIKPGGTIVEATSGNTGVGLAIAAALKGYKTIF
                     VMPDKMSNEKILLLRAYGAKVVITPTAVGPDDPRSYYEVAKKFARETPNAILANQYHN
                     PDNPKTHELSTGPEIWEQTDGKVTDVIIGIGTGGTITGVGRYLKAKNPNITIVGVDIE
                     GSILTEIWQNKGIIPPGAYPKTYKVEGIGEDFLPSTMDIKYVDAIERAGDRESFLWAR
                     QLVRQEGIFAGGSSGSAIAGAIKYCRKLQPGRLPVVILPDSGSRYLSKFYDDKWMREF
                     GFLSMEFGETALGDLLIAKPNKALITATLGDSIKKVVTVMHQNAVSQMPVVGTDGALV
                     GLIEEVDLLNHMLEKHDHSNEEKIDPLIQNAGAVFPPETPLEDAMPSLTAGYALIVVE
                     NSRPIGILTKIDVLDYVAGKI"
     gene            complement(2600..3010)
                     /locus_tag="PLT08_10710"
     CDS             complement(2600..3010)
                     /locus_tag="PLT08_10710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE04980.1"
                     /translation="MKAKKLSILLAAIMLVAVQLACAAGEPTFSNVRTATDQDGTQLS
                     TTFGSFDTVYVVGDLSNGVVGNNVTARLFVDQVDGFDPNFFVGEAAVDVDQDNINSVY
                     FYFEPPTDGWPVGTYKVEVYFNGTLNTTLSYSVQ"
BASE COUNT          826 a          790 c          659 g          788 t
ORIGIN      
        1 ccacggcgat gaaattcagg cgcgacatag aggcggtcga taaaatcatc ttttaacgct
       61 agaaaagcca caggcttgcc atccaactca tagacccaga gttggttttc agcaaggatg
      121 ccattgcgaa agtaattcag agaaccttcc aacgtgtacc ccatgcgttt cattaagtca
      181 gggatggcaa ccagcatggc attaaaccat aaggcggtga ctgcatcaaa gtctttttct
      241 tgatacggac ggatcatatt ctctccaata aaatacagcg gacggttaac cgtccgctgt
      301 attttacttc acggatgtta cgcagtaggg gcgaccctat cgcgttttgt gataactgtt
      361 agcaaagggc gactcttcct aataaattgg cataaggtta agccttgacg ggtcgcccct
      421 acgatgaaat ttcgtaaggg agttacttat acctaaccca tgatgccaac aatggcaccc
      481 atcactacga aagtgataag gtgattgcct tgttcaagca cccaagcctg taaacgatcg
      541 gcgaacaact tattggtcag gctcgaagat gccacaaaac caagccagat caagaatcca
      601 gtgagcgcgc cggaggcaag tgtcaccccg ccagagatac tacccatagc gtggaccatc
      661 atggacatga acacagattc gacgaaagcg gcaaagacga ccccacccca aaccgccccc
      721 atgccaagtg aggagccggg tgtgttctcg ccgccaccca tgctcggttc acccgctttg
      781 ccaacagctt tccaccaaat tgggaagaag gtcttgggtc cgaaccaaac gccgccaata
      841 accatgcttg ccagaacaca aaataaaaca gccaaccaat taatagaact gaaatccatt
      901 ttctttctcc ttgggacgaa aatttgtact ctcattataa tagaaaatat gttctaatca
      961 aattaaggaa ttttttcaca taacctttcc ctaaaagcaa aaggagaagc tttaccgctt
     1021 ctcctttttg tttaagttct tatttaccag taggttagat tttccctgct acataatcca
     1081 aaacgtcaat cttggtaaga ataccaatgg gtctgctatt ctcaaccaca attaaagcat
     1141 agccggctgt aagtgaaggc attgcatcct caagcggagt ttcaggtggg aaaaccgcgc
     1201 ctgcgttctg aatcaacggg tcaatctttt cttcgttgct atggtcgtgc ttctccaaca
     1261 tatgattcag caaatccact tcttcgatta acccaaccaa ggctccatct gtacctacaa
     1321 ctggcatttg cgatacagca ttctgatgca tcactgtcac gactttctta atcgagtcgc
     1381 ctagtgtggc ggtaatcaaa gccttattcg gtttcgcaat caacaggtca ccgagagccg
     1441 tttcgccaaa ttccatcgag aggaagccga attcacgcat ccatttatca tcataaaact
     1501 tggaaagata acgcgaaccc gaatccggca agataacaac cggcaaacgt cccggctgga
     1561 gtttgcggca atacttgatg gcacctgcaa tcgcagaacc cgatgagccg cctgcaaaaa
     1621 tgccttcctg tcgcaccagt tgacgtgccc acaagaaaga ctcacgatcc cctgcacgtt
     1681 caatggcatc gacatattta atgtccatgg ttgagggcag gaagtcttca ccgatgcctt
     1741 ctactttata ggtctttgga tatgctccag gaggaatgat gcctttattc tgccaaattt
     1801 cggtcagaat cgagccttcg atatccacac cgacaattgt gatgtttgga ttcttggctt
     1861 tgagataacg cccaaccccg gtgatggtgc cgccagttcc aatgccaatg atgacatcgg
     1921 tcactttgcc atcggtctgc tcccaaattt caggtccggt gctcagttca tgagtctttg
     1981 ggttatcggg gttgtgatat tgattcgcta aaattgcatt gggggtttcg cgggcaaact
     2041 tctttgctac ttcataataa gaacgtgggt cgtctggtcc gaccgcggtg ggagtaatca
     2101 caactttggc accatatgca cgtaacaata aaatcttttc gttgctcatc ttatcgggca
     2161 tcacgaagat ggttttatag cccttcagtg ctgccgcaat tgccaacccc acccctgtat
     2221 tcccggaggt cgcctcgacg atggtgcccc caggtttgat ctctccacgt ttttctgctt
     2281 gttcaataat attcgccccc accctatctt tgaccgaccc gccgggattc ataaattcca
     2341 gcttggcata tagtggaaca ggcaggtctt ttccaattcg ttccagtttc acgagcgggg
     2401 tatttcccat cgctccaagt acattgttga atacacgttt ctctttggat tctggcaatc
     2461 ctgtcgtcat tctctctcct ttggttttag ggaaattcta atcgacttcg ctcaccttcc
     2521 actgtattat aaaccgaggg tacagtaaaa caaaaagcgg ctgccttttc aggcagccgc
     2581 ttggcttaca caaaaaactt tattgaaccg aatagctgag ggtggtgttc aacgtaccgt
     2641 tgaagtacac ttccacttta taggtaccga ctggccaacc atcggtaggc ggttcgaagt
     2701 agaagtagac cgagttaata ttatcctgat ccacatccac agcagcttca cccacaaaga
     2761 agttcgggtc aaaaccatct acctgatcga cgaacaaacg agcagtaaca ttgttaccaa
     2821 ccacgccatt ggaaagatca cctacaacat aaacggtgtc aaaagacccg aaggtggtgg
     2881 atagttgggt accatcctga tcggttgccg tgcggacgtt cgagaacgta ggttcgccag
     2941 ccgcgcaggc gagctgaaca gcaactaaca taattgcagc cagcaagatc gacagttttt
     3001 tcgctttcat tttggcattc tcctcataat ataaatgtag gcaggtagcc ccgccaagtg
     3061 tcg
//
LOCUS       DAOQJE010000397         3057 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_21436_length_3057_cov_6.912059, whole genome shotgun sequence.
ACCESSION   DAOQJE010000397 DAOQJE010000000
VERSION     DAOQJE010000397.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3057)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3057)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3057
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21436_length_3057_cov_6.912059"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1481)
                     /locus_tag="PLT08_10715"
     CDS             complement(<1..1481)
                     /locus_tag="PLT08_10715"
                     /inference="COORDINATES: protein motif:HMM:NF024882.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK 4TM domain-containing
                     protein"
                     /protein_id="HNE04981.1"
                     /translation="MPLLKPSAPQSNSKKPAGKTLPAKKGGADLPSKAPAKGRGLPPQ
                     APPSPPPSYTPQPLREQTWWEKLSPERKLDVVGVILAFFGLVILLGLLSANRSAIVGG
                     AIFFLAQLFGWGVYVLPIGLLVFGLWLVFRKIERIPPLSTERAVGSVILFFWLMTLLH
                     SFIATAETAEAVALTGQGGGYFGGLAQRILWGAFGAGGAFIAMIAWLIIGVAVLLDKP
                     VIELFFWLKPLMLKLRDLIIKPAPQTQPVTAQPQSSDGLTPIDSTAMPAMDMTNAVPV
                     QPVQTRSGTTIIEWKLPDLTDILDEGSEATVNEDFIKQRAHLIEETLASFGAPAQVVA
                     ISRGPSITQFGVEPLFLESRGGVRTRVRVSKIASLADDLALALAAPRIRIQAPVPGHS
                     YVGIEVPNDEMARVALRDILEGEIFKGYKKPLAFALGRDVAGLPAISSIENMPHLLIA
                     GTTGSGKSVCVNSILTCMLLYNTPDDLRLVLVDPKRVELTGYNG"
     gene            1757..2677
                     /locus_tag="PLT08_10720"
     CDS             1757..2677
                     /locus_tag="PLT08_10720"
                     /EC_number="3.1.26.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016891 - endoribonuclease activity,
                     producing 5'-phosphomonoesters [Evidence IEA];
                     GO_process: GO:0042779 - tRNA 3'-trailer cleavage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Z"
                     /protein_id="HNE04982.1"
                     /translation="MFEILFLGTSASAPSAHRGLSSHIVSHNEYRFLVDCGEGTQRQI
                     LQSGKGFKRLTRALITHGHLDHILGLGGLLSTFLRWEAIEEFEVYGSKGTLERVKTLL
                     FDVVLRGKKTPMPLRLIEIKPGVIFDAEDFTVTAFSVSHGKADSLGYIFEEKARRPFL
                     AEKADALGVPFGPERKALVNGEAITLADGTHISPEEVLGDWEKGSKLVVVGDAGRTDN
                     LIEACQDADAVVIESTYLDEEADMASQFSHLTAKMGAELAQKAGVKKLILTHISRRYR
                     GRDVLAEAQTVFPNTVVARDFDTFVIKKDN"
     gene            2679..>3057
                     /locus_tag="PLT08_10725"
     CDS             2679..>3057
                     /locus_tag="PLT08_10725"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HNE04983.1"
                     /translation="MADKLTIGVLTSGGDAPGMNAAIRAVVRTALANNMNVIGVMHGY
                     EGLVNGEFKPMGARDVGGILQRGGTVLLTSRSKRFQEPTGQREAIRKMNEAGMDALIV
                     IGGEGSMNGANALAQQGVKVIGIP"
BASE COUNT          771 a          678 c          839 g          769 t
ORIGIN      
        1 ccgttatacc cggtcaactc cacgcgcttc gggtctacaa gcacaaggcg caagtcatcg
       61 ggggtgttgt ataaaagcat acaagtcaga atcgagttca cacacaccga cttaccagaa
      121 cccgtcgtac ccgcgatcaa caagtgcggc atattctcaa tgctagagat ggcaggcaac
      181 ccagccacgt cacgcccaag cgcaaacgcc aaaggttttt tatacccttt gaaaatttca
      241 ccttctaaaa tatcgcgcaa cgctacacgc gccatttcgt cattcggcac ttcgatgcca
      301 acataactat gccccggtac cggtgcttga atacggatgc gcggcgcggc tagtgcaagg
      361 gcgaggtcat cagctaagga tgcgatctta cttacgcgga ctctggtccg tacgccgcct
      421 cgggactcaa gaaatagcgg ctccacccca aattgcgtga tcgaaggtcc acggctgatt
      481 gccactactt gggctggcgc gccaaaggaa gcgagcgtct cttcaatgag atgtgcgcgt
      541 tgcttaatga aatcttcgtt gacggttgct tcactgcctt catcaagaat atctgtcagg
      601 tctggcaatt tccattcgat gatggttgtg ccactgcgag tttgaacggg ctgtacgggg
      661 acggcattcg tcatgtccat ggcgggcatg gcagtagaat ctatcggcgt gagtccatca
      721 cttgattggg gctgagcagt gaccggttga gtctgaggcg cgggtttgat aatcaggtcg
      781 cgcagtttca gcatcaacgg tttgagccag aagaaaagtt cgatgacggg tttgtctagc
      841 aaaacggcga cgccaattat gagccaggcg atcatggcga tgaaggcacc gcctgcgcca
      901 aaggctcccc aaagaatgcg ttgagccaaa ccgccgaaat acccgccacc ttgaccagtg
      961 agtgcaaccg cttcagcagt ttcagctgtg gcgatgaagg agtgcaacag agtcatcagc
     1021 cagaagaaaa ggatgacact cccaactgcg cgttctgtag aaagcggagg gatacgttcg
     1081 atcttacgga agaccagcca caatccaaag acgagcaagc caatgggtaa gacgtatacg
     1141 ccccagccaa atagttgtgc aaggaaaaag atcgcgccgc caacgatagc ggagcggttg
     1201 gcagaaagaa gccctaataa aatgacgaga ccaaaaaatg cgaggatgac acctacaaca
     1261 tctaacttgc gttcgggtga aagtttttcc caccaggttt gttcgcgcag gggttggggt
     1321 gtatatgatg gaggtgggga tggtggagct tgaggaggaa gccctctgcc ttttgccgga
     1381 gctttggaag gtaaatccgc gccgcctttt tttgcgggaa gagttttgcc tgcgggcttt
     1441 ttactattcg attgaggcgc ggagggtttt aggagaggca ttcgattttc aagaggaaat
     1501 aggttcgttc atcatttacg ataaaaaatc gttcttgagt cattatagca caaatggtct
     1561 gtttaacttc ttgtgctgat tctattgagt ttcatttcaa acgtattctt cattaaatgg
     1621 gtttttgccc aatagttgat ttgagttaag gtaaaattgc agggcttgtt gccagtttgg
     1681 ttgtgtgcga taagagtatt tttcagcgat tgattcgcgg ggctacattt cctccgcagt
     1741 ttgataggac acaattttgt tcgaaattct atttcttggt acatccgcat ctgcgccatc
     1801 ggcacaccgc gggctttctt ctcatatcgt ctctcataac gaatatcgtt ttttggtcga
     1861 ttgtggcgaa ggcacccagc gccagatatt acagtctggc aagggcttta agcggttaac
     1921 gcgtgcgctc atcacacacg ggcatctgga tcatattctg gggttgggtg ggttgctttc
     1981 cacttttttg cgttgggaag cgattgaaga gtttgaagtg tatggcagta agggaacgct
     2041 tgaacgtgta aagactttgt tgttcgatgt ggtgttgcgc ggcaagaaaa cgcccatgcc
     2101 tttgcgtttg atcgagataa aacccggcgt gatcttcgat gcagaagatt tcactgtaac
     2161 cgccttttct gtttcccatg gcaaggcaga tagcctaggc tatatctttg aagaaaaagc
     2221 acgccgtcct ttccttgcag aaaaagcaga tgctctaggt gtgccgttcg gacctgagcg
     2281 aaaagctttg gtgaatggcg aggcgattac acttgctgat ggaacacata tctctccaga
     2341 agaagtgctt ggggattggg aaaaaggcag taaactggtt gttgtgggtg atgcaggtag
     2401 aactgataac ttgatcgaag cttgtcagga tgccgatgca gttgtgattg agtctaccta
     2461 tctggatgaa gaagcggata tggcaagtca gttttcgcat ttgaccgcga aaatgggtgc
     2521 ggaactcgca caaaaagcag gggttaaaaa actaatactt acgcatattt cacgacgcta
     2581 tcgtggaagg gatgtgcttg cagaagcaca gactgttttt ccaaatacag tagtggctcg
     2641 tgattttgat acttttgtga taaagaaaga taattgatat ggcagataaa cttacaattg
     2701 gtgttttgac ttccggaggc gatgcccccg gcatgaatgc ggcgatccgc gcagttgtgc
     2761 gtacggcgtt agcaaacaat atgaacgtaa tcggtgtgat gcatggttat gaaggattag
     2821 taaacggcga gttcaaaccg atgggtgccc gtgatgtagg tggcatcttg caacgcggtg
     2881 gaacggtgct tcttaccagt cgcagcaaac gcttccagga acccactggt cagcgcgagg
     2941 cgatccgcaa gatgaatgaa gccggcatgg atgcattgat cgttattggt ggtgaaggct
     3001 ctatgaacgg cgcgaatgcc cttgctcaac aaggtgtgaa agtcatcggc atccccg
//
LOCUS       DAOQJE010000398         3057 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_21438_length_3057_cov_5.073951, whole genome shotgun sequence.
ACCESSION   DAOQJE010000398 DAOQJE010000000
VERSION     DAOQJE010000398.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3057)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3057)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3057
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21438_length_3057_cov_5.073951"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..240
                     /locus_tag="PLT08_10730"
     CDS             <1..240
                     /locus_tag="PLT08_10730"
                     /inference="COORDINATES: protein motif:HMM:NF014234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004665 - prephenate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0008977 - prephenate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0006571 - tyrosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase/arogenate dehydrogenase
                     family protein"
                     /protein_id="HNE04984.1"
                     /translation="PEEVAPFVGPGFKSTSRLAGTSSSMMLGVLQSNRENVLEALHRM
                     QNEFAQLEAALTAGDFVKVESLLNKAQAKHQSFTQ"
     gene            273..1538
                     /gene="aroA"
                     /locus_tag="PLT08_10735"
     CDS             273..1538
                     /gene="aroA"
                     /locus_tag="PLT08_10735"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: protein motif:HMM:NF012497.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003866 - 3-phosphoshikimate
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HNE04985.1"
                     /translation="MSHPLSATVRVPGSKSLTNRALLISSLANGTTHLTNTLFSDDSH
                     YFAKALQTLGFNVQLDEENFSMTVTGMGGKIPARKAELFIGNAGTAARFLSAFLTLGH
                     GEYILDGEPRMRERPIRDLIDSLSQLGAKLEPTNDCPPVKISANGLPGGKTTIAGDIS
                     SQFLSALLMVAPYAKSPIEVTLSTDLNSKPYVDMTISIMKDFGVEVERDGYEYFKVPV
                     TSYQLPVTSYPIESDASAASYFFAAPAICGGTVTVENISHKSVQGDVAFVDVLKQMGC
                     AVEEHDNSILVTRASSLVGIDVDMRDIPDTAQTLAAIAPFASSPTRIRGIASARVKET
                     DRVHATCVGLDGLGVRVEEHEDGMTIYPVEKMRSASIQTYNDHRMAMAFSLIGLRFDG
                     VTIENPSCVSKTFPNYFEVLEELRTQGNK"
     gene            1546..2385
                     /gene="aroE"
                     /locus_tag="PLT08_10740"
     CDS             1546..2385
                     /gene="aroE"
                     /locus_tag="PLT08_10740"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: protein motif:HMM:TIGR00507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004764 - shikimate 3-dehydrogenase
                     (NADP+) activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HNE04986.1"
                     /translation="MMNYQLGLIGYPLGHSLSPKIHTAALVACGLQGTYSLFPIHPDN
                     KQGLHNLLAQVRAGEIRGLNVTIPHKQNVIEFMDELTPTAKAIGAVNTIYMRADKLIG
                     DNTDAPGFLSDLKRVSNSSFSTPLSALVLGAGGSARAVVYALANDGWNVTLAARRVEQ
                     AQQLASSIDNYQLSIVNYSNLQPSTFDLLVNTTPLGMTPNTEASPLPEGTELSKNTFV
                     YDLVYNPRETKLVRDARAQGLNASTGLGMLIEQAALAFEKWTGHNPPREILFEAVRAT
                     RPD"
     gene            2440..>3057
                     /gene="aroC"
                     /locus_tag="PLT08_10745"
     CDS             2440..>3057
                     /gene="aroC"
                     /locus_tag="PLT08_10745"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HNE04987.1"
                     /translation="MLRFLTAGESHGPSLTTILDGIPAGLPLSTDIINKELARRQQGY
                     GSGGRMKIEKDTVQILGGVMGGETTGAPIALLVQNDDHIKWKGKAIEPMTAPRPGHAD
                     LTGAVKYGYKDLRPALERASARETTMRVAAGAVCKHFLAQFGIIIGGYVASIGEVTAD
                     FGDMPYEERFIRAEESDVRCPIDSSADKMRAEIEKTIHGKNTLGGV"
BASE COUNT          773 a          850 c          685 g          749 t
ORIGIN      
        1 cctgaagaag tcgctccctt tgtcgggcct ggcttcaagt ccacgagcag acttgctggc
       61 acatcttcct ccatgatgtt gggcgtttta cagtccaacc gcgagaatgt gttggaggcg
      121 ttgcatcgaa tgcaaaatga atttgctcaa ctcgaagccg cactaaccgc tggcgatttt
      181 gtaaaagtcg aatcccttct aaacaaagcg caagccaaac accaatcttt cactcaatga
      241 atattcatca ttcatcattc cgcattcatc ctttgtctca tcctctctcc gccaccgtcc
      301 gcgtacctgg ctccaagtca ttgaccaacc gcgccctgct gatttcgtcc cttgcaaacg
      361 gcacgaccca cctcaccaat acactcttct ccgacgactc gcattacttc gctaaagccc
      421 tgcaaaccct cggctttaat gttcaattgg atgaagaaaa tttttccatg acggtcacgg
      481 gcatgggagg gaaaatccct gccaggaaag ccgaactctt tatcggcaat gcgggcactg
      541 cggcaagatt tctctccgcg ttcctcacac ttgggcatgg cgaatacatt ttggatggcg
      601 aaccgcgcat gcgcgaacgc ccaataaggg atttgattga ttcgttgtca caacttggcg
      661 caaaactcga accgacaaat gactgtccac ccgtaaagat ttcagcaaat ggcttgcctg
      721 gtggaaagac aaccattgct ggtgatattt cttctcaatt cttatccgca ttgctgatgg
      781 tcgctcctta tgctaaaagc cctattgaag tcactctctc cactgatctc aactctaagc
      841 cctacgtaga catgacaatt tccatcatga aagacttcgg tgtggaagtt gaacgggatg
      901 gatatgaata tttcaaagtg ccagtgacca gttatcagtt accagttacc agttatccaa
      961 ttgaaagtga cgcctccgct gcgtcctatt tcttcgccgc ccctgccatc tgcggcggga
     1021 cagtcacagt agaaaacatc tcgcacaagt ctgtgcaagg cgatgttgct tttgtagatg
     1081 tgctaaaaca aatgggttgt gctgttgaag agcacgataa ttcgatactc gttactcgtg
     1141 cctcgtcgct tgttggcatt gacgtagata tgcgcgatat tcccgacacc gcccaaactc
     1201 ttgctgcgat cgccccattt gcttcatcac caaccagaat tcgtggcatc gcatctgcca
     1261 gagtcaaaga gacagatcga gtccacgcga cctgtgttgg gttggacgga ctcggcgtcc
     1321 gcgttgaaga gcatgaagat ggaatgacca tctatcccgt agaaaagatg cgcagcgctt
     1381 ccatccaaac ctataacgat catcgcatgg caatggcatt ctctctcatc ggcttacgct
     1441 ttgatggtgt caccatcgaa aatccatctt gtgtttcaaa gacatttcct aattactttg
     1501 aagtgcttga agaattgcgt acccaaggaa acaagtaaac aggtgatgat gaattatcaa
     1561 cttggtttaa tcggttatcc cttgggacac tcactatcgc caaagattca tacagcggct
     1621 ttagtcgctt gcggcttaca aggaacctat tccctattcc ctattcaccc tgacaacaaa
     1681 caaggcttgc acaacctgct cgcccaagtt cgtgctggcg aaattcgcgg actcaacgtc
     1741 accattccgc acaaacagaa cgtcatcgaa ttcatggacg aactcacgcc caccgccaaa
     1801 gccatcggcg cggtcaatac gatttatatg cgtgcagata aactcattgg cgataatacc
     1861 gatgcgccag ggtttctctc tgacctcaag cgggtttcaa attcatcatt cagcactccg
     1921 ctttctgcat tggtcttggg tgcaggtggt tctgcgcgag ccgtagttta tgctttagcc
     1981 aatgatggct ggaatgtcac ccttgccgcc cgtcgcgtcg aacaagccca acaactcgca
     2041 agttcaattg ataattatca attatcaatt gtcaattatt caaaccttca accatcaacc
     2101 ttcgacctgc tcgtcaacac caccccgctc ggcatgacgc ccaataccga agcctccccc
     2161 ttgcccgaag gtactgaatt atcaaaaaat acttttgttt atgatctggt ctacaatcca
     2221 cgcgagacca aacttgtccg cgacgcccgc gcacagggat tgaacgcctc cactggtctg
     2281 ggcatgctca tcgaacaagc cgccctcgcc tttgagaaat ggacagggca caacccgccg
     2341 cgtgaaattt tatttgaagc tgtaagggcg acccgccctg attgatcaaa actccggacg
     2401 accacaaagg gtcgcccctg ctttatgaat ggagaacgta tgcttagatt tttaactgct
     2461 ggtgaatcac atggaccatc gttgactaca attttggatg gtatccctgc tggtttgcct
     2521 ttatctactg acattatcaa caaagaactc gcccgccgcc agcaaggcta tggctcaggc
     2581 ggacgcatga aaatagaaaa agataccgtg caaattcttg gcggcgtgat gggcggcgaa
     2641 accacaggtg cgccgattgc cctgctcgtc caaaacgatg accatatcaa atggaaaggc
     2701 aaagccatcg agccgatgac cgctccccgc ccgggtcacg cggatttaac aggcgcggtc
     2761 aagtacggct acaaagatct gcgacctgca ttggaacgtg cttccgctcg tgagactacc
     2821 atgcgtgttg cggcgggtgc ggtctgcaaa catttcctcg ctcaatttgg aatcattatc
     2881 ggcggatatg tcgcctccat cggtgaagtg accgcagact ttggcgacat gccttacgaa
     2941 gaacgcttta tccgcgccga agaatcggat gtgcgttgcc cgatcgattc ctccgctgac
     3001 aaaatgcgcg ccgaaatcga aaagaccatc cacggcaaga acacgctcgg cggcgtg
//
LOCUS       DAOQJE010000399         3056 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_21448_length_3056_cov_6.249917, whole genome shotgun sequence.
ACCESSION   DAOQJE010000399 DAOQJE010000000
VERSION     DAOQJE010000399.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3056)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3056)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3056
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21448_length_3056_cov_6.249917"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..393
                     /locus_tag="PLT08_10750"
     CDS             <1..393
                     /locus_tag="PLT08_10750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5915 domain-containing protein"
                     /protein_id="HNE04988.1"
                     /translation="VDGTDYGILAEEVEVKAQAKSGFAVAEDGAYVAALVTDLTPELV
                     AEGLAREFVRRVQDLRKTADLDVSDRIDLFIEASAGLKTAVEAHRDYITAETLTKSMT
                     FATPPADAAVVEDEFEGEKVKVGLVKSK"
     gene            complement(491..1213)
                     /locus_tag="PLT08_10755"
     CDS             complement(491..1213)
                     /locus_tag="PLT08_10755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE04989.1"
                     /translation="MNNKYENPLNIATSKEFLLLAYKDYIAARHLLRNEMLLQGTILA
                     STCLEKYFKAILILKSKRYTKHLSQTLCNEIKDFDGSLYNKLDQSFLNLLIKCYRLRY
                     LDNITDTFSVILVQYQLLAELDYTVSKIEGNIHIYQNDQKLILNYEQAWREKDKSLVD
                     ENHIYHGVSSDKFIQEKESFIYCIRIDPNLSFNKTIIESHGHIAKQPIDKDFMREFKI
                     SDYPTEENPKTGFLIPFKLRNS"
     gene            complement(1284..2291)
                     /locus_tag="PLT08_10760"
     CDS             complement(1284..2291)
                     /locus_tag="PLT08_10760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE04990.1"
                     /translation="MKKHENKNNNDTLVECPFCGLKVYSRNLAKHAKLLHAGTKKPSQ
                     TLVAGGSYSINGVEIQNKDLAQSLNQLVVDENGVLINPKLLQEEARISRNEVMGTAQK
                     PKQQLAKSKKEQNRTIYKINQKHKSIDELYREKEEWLKKIPQLNSISKNKNALIVCPV
                     CNAKVKSKRLWRHFAKNHQGIHLTNEQINKLENGEITKIDLSTQRKPSKINHPVGLSR
                     NNTSSDENEISNLVKCPLCKHKTFYNVLFTHIQVSHPEVNPKIMMAKFNKVYRNKNTL
                     SNSNYEKEINEVVKEYEKLKQGQDETRDGGKYLGFMRRESGKFGSLPLYDDYSDDADA
                     E"
     gene            complement(2317..>3056)
                     /locus_tag="PLT08_10765"
     CDS             complement(2317..>3056)
                     /locus_tag="PLT08_10765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE04991.1"
                     /translation="CMTIDRFVSELSKTPWIRPTAIVDWYYAVYFSIRAILPIFGHQI
                     PEDHSKTAKFVASTFRTHLPYPFDMFARRTIGEKYNTYIGGLQLQHYDLNRSFYSMSS
                     ISQGMLAQYLGGTAEWYAIRTKKNILADKKKGISNFRTKNAREERDRRLTSEIGFLHC
                     AYRQRTKANYRDAIYLAYHYGEEIGLENFMSNLSASAKFASINAIACTEYFIGRNDVK
                     AFIGDLSVNLRGIDIAGPSEKYWSEFL"
BASE COUNT          803 a          590 c          600 g         1063 t
ORIGIN      
        1 gtggacggga ccgactacgg cattctggcc gaggaagtgg aagtgaaggc gcaggcgaaa
       61 tcgggctttg cggtggcgga agatggcgcc tatgtggcgg ccctcgtgac cgacctgacg
      121 cccgaactgg tggccgaagg attggcgcgt gagttcgtgc gccgcgtgca ggacctgcgc
      181 aagaccgccg acctggacgt ctctgaccgc attgacctgt tcatcgaagc cagcgccggg
      241 ttgaagaccg ccgtggaagc gcatagggac tacatcaccg ccgaaacgct gaccaagtcc
      301 atgacctttg cgaccccgcc cgcggacgcc gctgtggtgg aggatgagtt cgaaggcgaa
      361 aaagtgaagg tggggctggt gaagagcaag tgaccttgag tcattcgtga gccgtagata
      421 gtaggcagta aaatataaga cctcggagtc tcatcgactc cgaggtcttc ttcgaatccg
      481 ctgtgatagg tcaagagttt ctcaacttaa agggtattag aaacccagtt tttggatttt
      541 cttccgtcgg gtaatcagaa attttaaact cacgcataaa atctttatca attggctgtt
      601 tcgctatatg accgtgagat tctataattg ttttattgaa acttagattt ggatctatcc
      661 tgatgcaata tataaaactt tccttttctt gaataaattt atcgctagaa acaccgtggt
      721 agatgtgatt ttcatctacg aggcttttat ctttttcgcg ccacgcttgt tcgtaattta
      781 atattaattt ttgatcgttt tgatatatat gaatgttccc ttcaatttta cttactgtat
      841 agtctaattc cgcaagtaat tggtattgaa ccaatataac gctgaacgta tcggtgatat
      901 tatccagata ccgcaatcta tagcatttta ttaatagatt caaaaatgac tgatctaatt
      961 tattgtacag gcttccatcg aaatctttga tttcattaca cagggtttgt gacaaatgct
     1021 ttgtatagcg cttgctttta agtatcaaaa ttgctttgaa atatttttct agacatgtgc
     1081 tggcaagaat tgttccttgt aaaagcattt cgtttctcag taagtgcctt gcggcaatat
     1141 aatctttgta tgcgagaagg agaaattcct tagaagtagc tatattaaga ggattttcgt
     1201 atttattatt catcttttta attgtgattt ctcaaatttc tatcagcgcg gaggttgacc
     1261 acagtctcca taaattcact ttactattcg gcgtctgcgt catccgaata atcatcgtaa
     1321 agcggcaaac taccgaactt accactttct ctgcgcataa accctaaata ctttccacca
     1381 tcacgagttt cgtcttgccc ttgtttcagt ttttcatatt ctttgactac ttcatttatt
     1441 tctttttcgt aattcgagtt gcttaatgtg tttttatttc gataaacttt attaaatttt
     1501 gccatcataa tttttggatt tacctcaggg tggcttactt gaatatgagt aaaaagtaca
     1561 ttgtaaaatg ttttgtgttt acataagggg cattttacta aattcgatat ttcgttctcg
     1621 tcagatgatg tgttattgcg gcttaatccg acaggatgat taattttgga aggttttcgc
     1681 tgggtactta aatcaatctt tgtaatttct ccattttcca atttattaat ttgttcatta
     1741 gtgagatgaa tcccttgatg gttttttgca aaatgtctcc ataatcgttt cgacttgacc
     1801 tttgcgttgc aaaccggaca aactatcaat gcatttttat ttttagaaat agaatttaat
     1861 tgtgggatct tttttagcca ctcttctttt tcacgataca gttcatcaat ggacttatgt
     1921 ttttgattaa tcttgtagat agttctgttt tgttcttttt tgctttttgc aagttgctgt
     1981 tttggctttt gtgcagtccc cataacttcg ttacggctga tgcgtgcctc ttcttgcaat
     2041 aattttggat ttatcaaaac cccattttca tcaacaacta attgattgag actttgcgct
     2101 aagtctttgt tttggatttc cacaccattg atagaatatg agcctcccgc cactagagtt
     2161 tgactaggtt tttttgtacc agcatgaagt aatttcgcat gtttggcaag gtttcttgaa
     2221 tacactttta agccgcaaaa tgggcactca actaatgtat cgttattatt tttattttcg
     2281 tgttttttca tatttgattt tctcccactt gcctgtctat aaaaactcgc tccaatattt
     2341 ttctgatggt cctgcaatat caattccgcg taaattgaca cttaaatccc caatgaatgc
     2401 tttgacatca tttcgaccaa taaagtactc ggtgcaagca attgcattaa ttgaggcaaa
     2461 tttagcggat gctgaaagat tactcataaa gttttctaga cctatttctt ctccataatg
     2521 atatgcaaga taaattgcat ccctgtaatt tgctttagtt ctctgccggt atgcgcaatg
     2581 taaaaagcct atttcagatg tcaaacgtct atccctttct tctctggcgt tttttgtgcg
     2641 gaaattggaa attcctttct tcttatccgc aagtatgttt tttttggttc gaattgcata
     2701 ccactccgca gttccgccca gatattgagc gagcatacct tgtgaaattg aactcattga
     2761 ataaaaagaa cgatttaaat cgtaatgttg aagttgaaga ccgccaatat atgtattgta
     2821 tttttcacca attgttcttc tcgcaaacat atcaaaagga taaggaagat gggttctgaa
     2881 tgtgcttgca acaaattttg cggttttgga atggtcttcc ggaatctgat gcccaaagat
     2941 tggcaatatc gcacgtattg aaaaatagac agcgtaatac caatctacga tagcagtagg
     3001 acgtatccaa ggagttttgc ttaattcaga tacaaatcgg tctatggtca tacaat
//
LOCUS       DAOQJE010000400         3055 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_21456_length_3055_cov_4.454667, whole genome shotgun sequence.
ACCESSION   DAOQJE010000400 DAOQJE010000000
VERSION     DAOQJE010000400.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3055)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3055)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3055
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21456_length_3055_cov_4.454667"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..310
                     /locus_tag="PLT08_10770"
     CDS             <1..310
                     /locus_tag="PLT08_10770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE04992.1"
                     /translation="QGVKTPVWFARDGETLRIWTQADAGKAKRIRQDGKVRIVPSTAS
                     GEPVGEWTDAKAAVLVSSEEVQHTAALFKQKYGLMFNVFGVMGKLRGAKFITIQVELG
                     "
     gene            383..1030
                     /locus_tag="PLT08_10775"
     CDS             383..1030
                     /locus_tag="PLT08_10775"
                     /inference="COORDINATES: protein motif:HMM:NF020893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HNE04993.1"
                     /translation="MIEQTILHLLQKLYDELGWLGVTIIMIIENATGIAPSEVVLALA
                     GWMLIDAHSLPFSTVFLGGLFAAFGSVMGASILYWAARLGGRPVVERVTHFFRIDLRH
                     MQRVERLAEKHGTRFIFFGRLIPGIRMLVAIPAGLTRMPYLTFAIATFLGAYLWCTVF
                     ISVGYFLGHEWHLISNIVKQIGPYAFGIAVFFTGIFFIWRRWQRQKLETVKLEIE"
     gene            complement(1067..2431)
                     /locus_tag="PLT08_10780"
     CDS             complement(1067..2431)
                     /locus_tag="PLT08_10780"
                     /inference="COORDINATES: protein motif:HMM:TIGR00797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HNE04994.1"
                     /translation="MFKSLSFLKDRTFLNEMLVIALPISFQQLINASLNMIDVIMVGQ
                     LGETSIAALGLSNQVFFVFILLLFGLTSGMAIFTAQFWGKQEVDPIRKVLGMSLAITS
                     AIALLFTLAATLIPRTVLGLYTHDKEVIEIGASYLSIVGFSYIPVAIATSYIATLRSI
                     QLVKLTVIATISALIFKTILGYILIFGVFGLPALGVRGAAIGTASGWTLELILLIILV
                     YAQKTPLAANPLTFFSFDFSFFGRVLKTTLPALANEMFWSLGITTYNAIYARIGTDSI
                     AAINVNATIEELGFVVFMGLGNACAVMVGNRIGAGKKEEAFETVRRVVILALLSAWTV
                     GLIVLFLRDIVVNLYDLSPSGEYNVRMLMLMMACTLWIRMFNFSTFIGALRAGGDTRF
                     ALLMELCSIWLIGVPAAYIGAFVLHLPVYFVYLMVALEEVAKAFVSGWRVYSRKWIHD
                     LVNV"
     gene            complement(2524..>3055)
                     /locus_tag="PLT08_10785"
     CDS             complement(2524..>3055)
                     /locus_tag="PLT08_10785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNE04995.1"
                     /translation="WTASMFYRLIYRITDVKIPVDTGDFRLMDRKVVNVLKQMKERHR
                     FPRGMSAWVGFKQIGVTYKRAARHAGVTKYPLRKMLRLAINAITGFSYFPLQVATYIG
                     FISAGISIFAIPVVVIMRMAGSGAFFGQATTLIAVLFLGGVQLISLGILGEYVGRLYD
                     EAKGRPLYIVRDAPEE"
BASE COUNT          903 a          698 c          726 g          728 t
ORIGIN      
        1 acaaggggtt aaaacaccgg tttggtttgc acgggatgga gaaacgcttc gcatctggac
       61 acaagccgat gcaggcaaag ccaagcgtat ccgccaggat ggcaaagtgc gtatcgtacc
      121 gtccacggct tcgggcgaac cagttggcga atggaccgat gccaaagccg ctgttcttgt
      181 ttcttcggaa gaggttcaac atacagccgc tttattcaaa cagaaatacg gtttgatgtt
      241 taacgtcttc ggagtaatgg gaaaattacg tggcgcaaaa ttcatcacca ttcaggtgga
      301 gcttggctaa tttctttctc atcttattta aaccgttctt taactttctc cggtactatg
      361 gagaaatgag gtttgagaga gaatgatcga acaaacgatc cttcatttgc ttcaaaagtt
      421 atacgatgaa ttgggctggc tcggggtcac cattattatg atcatcgaaa atgcaacagg
      481 catcgctccc agcgaggtgg tgcttgccct tgccggttgg atgttgattg acgcgcatag
      541 tttgcctttt tcaaccgtgt ttctgggagg gcttttcgcc gcattcggaa gtgtaatggg
      601 agcatcgatc ttatactggg cagcacgcct cggcggacgc cctgtagtag agcgggttac
      661 tcattttttt cgcatcgacc tgcgccacat gcaacgagtt gagcgccttg ccgaaaagca
      721 tggcacacgg tttattttct ttggcaggct aattccaggc attcgaatgt tggttgccat
      781 tcctgccggg ctgacgcgta tgccctacct cacgtttgca atagctactt tcttgggtgc
      841 atacctttgg tgtacagtat tcatcagtgt tggttacttc cttgggcatg agtggcatct
      901 tatcagcaat atcgtcaaac agataggacc ttatgccttt ggcattgccg tatttttcac
      961 aggtattttc tttatctggc ggcgatggca aagacaaaaa ctagaaacag taaagcttga
     1021 aattgaataa caaaaagagg tgctctcggt tgagagcacc tcttttttag acattcacaa
     1081 ggtcatgtat ccattttcgc gaatagacac gccatccgct cacaaaggct ttcgcgactt
     1141 cttccaatgc gaccattaga taaacaaaat agactggtaa atgcagaaca aatgccccaa
     1201 tgtatgctgc cgggacgcca ataagccaga tggagcataa ttccattaga agtgcaaagc
     1261 gggtatcacc gccagcccgc aaggcgccga taaacgtcga gaagttgaac atacgtatcc
     1321 acaacgtaca tgccatcatc aacatcagca tgcggacgtt atattcgccg ctaggtgaaa
     1381 ggtcgtataa gttcacaaca atatcacgca agaacaaaac gattagaccg accgtccatg
     1441 cagaaagaag agcaaggatc actactcgtc gaacagtttc aaaagcttct tcttttttgc
     1501 cagcaccaat acggttccca accatgacgg cacaggcatt ccccaacccc atgaatacta
     1561 cgaaaccaag ctcttcaatc gtcgcattga cattgatagc ggcaatagag tctgtgccga
     1621 tgcgggcgta gatagcgtta taagttgtaa tgcccaacga ccagaacatt tcattggcaa
     1681 gtgccggcaa ggtggtcttt agaacgcgac caaagaaaga aaagtcgaaa gagaaaaagg
     1741 tcaacgggtt ggcggcaaga ggagtcttct gagcgtagac aaggataatg agtaggatca
     1801 attccaaggt ccagccagag gcggtgccaa tggcggctcc acgaaccccg agggcgggca
     1861 atccaaaaac gccaaaaatc aaaatgtatc caaggatggt tttaaagatc aaagcggaga
     1921 tagtggcaat cactgtcagt ttgacgagtt gaatgctgcg caatgttgcg atataggaag
     1981 tagcaattgc cacaggaatg taggagaaac cgacaatact gagatatgaa gcaccgattt
     2041 caatcacttc cttatcgtgt gtataaagac caagaacagt acgtggaatc agtgtagcgg
     2101 caagcgtaaa cagcaaagca atggcagatg tgattgccaa actcatgccc aacactttgc
     2161 gaattgggtc tacttcttgc ttgccccaga attgagcagt aaaaattgcc ataccgctag
     2221 taagaccaaa aagcagcaat ataaatacga agaagacctg gttcgacaac ccaagtgcag
     2281 caatggatgt ttcgcccaac tgccccacca tgatgacgtc gatcatatta agcgaagcat
     2341 tgataagttg ttgaaacgaa attggcaatg caatgaccag catctcgttt agaaatgtac
     2401 ggtctttcaa aaaggaaaga gatttgaaca tagctgccta gtaactatag ttttttacat
     2461 aaagggataa aaaggtcgtt gacaataatt gtcaacgacc tttcaatcaa cgatctaaga
     2521 atactattct tcaggcgcgt cacgaacgat atagagaggt ctacctttgg cttcgtcgta
     2581 gagacgaccg acatactcgc caagaatacc gagcgagata agttgaacac cgccgaggaa
     2641 taatacagca atcaaggtgg tagcttgccc aaaaaaggcg ccgctccctg ccatacgcat
     2701 gatcactacc actggaattg caaaaatgga gatgcccgct gagatgaagc caatgtaagt
     2761 tgcaacctgc aacggaaaat atgaaaaacc agtaatggcg ttgatagcga ggcgcaacat
     2821 ttttcttaat ggatatttcg tcacaccggc atgacgggca gcacgtttat aagtgacgcc
     2881 gatctgcttg aagcccaccc aagcagacat accacgcggg aaacgatgac gctctttcat
     2941 ttgcttgaga acgttcacca ctttgcggtc catcaagcgg aaatcaccgg tatctaccgg
     3001 aattttcacg tcggtgatgc ggtaaatgag gcgatagaac atggacgccg tccac
//
LOCUS       DAOQJE010000401         3054 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_21468_length_3054_cov_6.360120, whole genome shotgun sequence.
ACCESSION   DAOQJE010000401 DAOQJE010000000
VERSION     DAOQJE010000401.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3054)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3054)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3054
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21468_length_3054_cov_6.360120"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..366)
                     /locus_tag="PLT08_10790"
     CDS             complement(<1..366)
                     /locus_tag="PLT08_10790"
                     /inference="COORDINATES: protein motif:HMM:NF025054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNE04996.1"
                     /translation="MAKNISLNDIHIRTDLQPGDTGGIMSMHGRLYYKEYGYTTVFES
                     YVAKGLGEFLEKYDPKRNRIWVCEHAGNMVGTLVLMDRGEAAQLRYFLLEPQYRGFGL
                     GTRLMDLFMEFFRDCGYASS"
     gene            462..830
                     /locus_tag="PLT08_10795"
     CDS             462..830
                     /locus_tag="PLT08_10795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE04997.1"
                     /translation="MKFYYWHYGIIFCSLATAILHIMCYPYFGWLDSIVLNGFGSIAL
                     LLAYFLPIPFFRRNRQTTYWIIVSYTLLTIVLWIFLGDKAFNFENTSSIGYFAKIAEM
                     FLLIFMGFDFPRKPAPARAS"
     gene            911..1753
                     /locus_tag="PLT08_10800"
     CDS             911..1753
                     /locus_tag="PLT08_10800"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HNE04998.1"
                     /translation="MKSIVVTGVSTGIGRSISELLIQNGFHVFGSVRKQADAERLKAD
                     LGTGFTPLTFDVTDEVAVQAAANQVRDALNGETLFGLVNNAGIAVAAPILHIPIEDFR
                     KQMEINVTGQLIVTQAFVPLLRAKDHQSKAGRVVNISSVSGKNGSPFLGAYAASKHAL
                     EGLSESMRRELMLYGIDLVIVAPGPIATPIWDKAEELKAEQYQHTDFYEPGKKFQKYA
                     IANGRKGLPPEAVSKIVFKALTLSHPRVRYAVDPNPFSNWVMSILPKRTIDKILAKGL
                     GFNK"
     gene            1819..2304
                     /locus_tag="PLT08_10805"
     CDS             1819..2304
                     /locus_tag="PLT08_10805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE04999.1"
                     /translation="MANGQVKKIATPKAAGYFIVYKVQPTDKSLKQIAREQLADEKLW
                     EKIVLDRETANPKGFFWDEIPANSFDPRWTLLLPPSEFAAFTISVNTPIHRAPNGEFL
                     AGANITEKYFYARKTVVVSGQKVWADVTKTNPARFGNKPYWILVKDGTSKTNPPISGP
                     S"
     gene            2438..>3054
                     /locus_tag="PLT08_10810"
     CDS             2438..>3054
                     /locus_tag="PLT08_10810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE05000.1"
                     /translation="MKTIVRSLALLSIASLLLASCTLFGSSKEPAANALSEVQATQSL
                     TDLVTIELTTQYDTAAVYNTVGQTVRFKYLVKMVKNDLPDGTPANVTITGITATCPAL
                     NTINNLNDRFDPGEVLECTGDYLITQADLDKGSIANVVTANAYSVNSNTVTTTVPTVS
                     PKALTLTKTASTTTYGAANQQITFTYIVKNSGTVQLGPAQFTIADT"
BASE COUNT          874 a          745 c          631 g          804 t
ORIGIN      
        1 ggacgaggca tacccacaat ctcgaaaaaa ctccataaaa aggtccatta ggcgcgtacc
       61 taagccaaat ccgcgatact gtggttccag caagaaatag cgcaactgtg ctgcttcgcc
      121 acgatccatt aagactaaag ttccaaccat atttccagca tgttcacaaa cccaaatgcg
      181 gtttcgttta gggtcatatt tttctaagaa ctcgcccagc ccttttgcta catagctttc
      241 aaaaacagtg gtgtatccat attctttgta gtacaaccga ccatgcatcg acataattcc
      301 acctgtatcg ccgggctgta aatcggttcg gatgtggatg tcattcagtg aaatattttt
      361 cgccatgagg cgcaatcata tcatcgaata tttgcttgcc tttgtaataa atctcccata
      421 aaatacgtct actaaaaaac tttatacgtg gagcattatc aatgaaattt tattactggc
      481 attacggtat catcttctgc agtcttgcta ctgccatcct gcacattatg tgttacccct
      541 acttcggctg gctagactcg atcgtgctaa atggttttgg ctcaatcgct ttattgctgg
      601 catattttct acccatccca tttttcaggc gtaaccgtca aaccacctat tggattatcg
      661 tcagctatac cctgcttacc atcgtgcttt ggatcttcct cggcgataaa gcgttcaact
      721 ttgagaacac ttcctccatc ggctattttg ccaaaatagc cgaaatgttc ttgctaatct
      781 tcatgggctt cgactttcca agaaagccag cacccgccag agcatcataa aataaaagag
      841 acagcacaaa gctgtctctt ttattttata ggtaaaatat cagtatatcc acgttcaaaa
      901 ggatctcttg atgaaaagta ttgttgttac aggggtatcg acaggaatcg gacggtcgat
      961 cagtgagtta ttaatccaga acggatttca tgtcttcggc agtgtacgca agcaggcgga
     1021 tgcagagcgc ctcaaagcgg acctcggaac tggcttcacc ccattgacct ttgatgttac
     1081 ggatgaagtc gccgttcaag ccgctgccaa tcaagtacgt gacgctttga atggcgagac
     1141 tttgtttggc ttggtgaaca acgcagggat tgcggttgcc gcaccgattt tgcacatccc
     1201 catcgaagat tttcgcaagc aaatggagat caatgtcacc gggcaattga ttgtcaccca
     1261 agcatttgta cctttgttac gagcaaaaga tcatcaaagc aaagccgggc gggtcgttaa
     1321 tatctcatct gtttcaggca aaaatggcag tccgttttta ggtgcgtatg ccgcctctaa
     1381 acatgcattg gaaggcttat ccgaatccat gcgccgcgaa ctgatgctct acggcattga
     1441 cttggtcatt gtagcgccgg gtccgattgc tacgccaatc tgggataaag ccgaagaact
     1501 aaaagccgag caatatcagc atactgattt ttatgagccg ggcaagaagt ttcaaaaata
     1561 cgccatcgcc aatgggcgca aaggtcttcc acctgaagct gtgagcaaga tcgtattcaa
     1621 agcgttgact ctttcccatc cgcgcgtgcg ctatgcagta gaccccaatc cattttcaaa
     1681 ttgggtcatg tcaatcttac ccaaacgtac aattgataaa atcctcgcaa agggacttgg
     1741 cttcaacaaa taaagctatt gacatccact tctattcggg tattctatag ttgtggttca
     1801 aaaacaaagg agagaaaaat ggcaaacgga caagttaaga aaatagcaac tccaaaagca
     1861 gccgggtatt ttattgtata taaagttcag cctacagaca aaagcttgaa acaaattgct
     1921 cgtgaacagc ttgccgatga aaagctctgg gagaagatcg ttctagaccg tgaaaccgcg
     1981 aatcccaaag gctttttctg ggacgagatc cccgccaact ctttcgaccc acgttggact
     2041 ttgttgctgc cgccttctga atttgcagcg tttaccatct ccgtcaacac gcctattcat
     2101 agagcgccta atggagaatt tctggctggc gcgaatatca ctgagaaata tttttatgcg
     2161 cgcaagacag tcgttgtcag tgggcaaaaa gtttgggcag atgtgaccaa gacaaatccc
     2221 gcacgattcg ggaacaagcc ttactggatc cttgttaagg atggtacatc caaaacaaat
     2281 ccacctatat ccggtcccag ttaatttgtt gctttaatcc tcgcattatc atcccgcaga
     2341 gtagacttaa gaaccctgcg ggatgattct taatccagat gtatagagga tataataaaa
     2401 gtggtcgtac aattttacaa aacgaaagga agtggtcatg aaaaccatcg tccgctcgct
     2461 tgccctgttg agcattgcca gtttactgct ggcatcttgc acgctctttg gcagctcaaa
     2521 agaacccgcc gcaaatgcat tgtctgaagt tcaggcaact caaagcctta cagatttagt
     2581 cacgattgag cttacaactc aatacgatac agcagcagtc tacaataccg ttgggcaaac
     2641 tgttcgcttt aagtatctcg tcaaaatggt caaaaatgac ctgcctgacg gcactccggc
     2701 aaatgttacg atcacaggca ttacagccac ttgcccggcg ctcaacacca tcaataatct
     2761 caatgaccgc ttcgaccctg gcgaagtgct tgaatgcacc ggagattatc ttattaccca
     2821 agcagacctc gataaaggtt caatcgcaaa tgtagtcact gccaatgcct attcggtgaa
     2881 ttcaaacaca gtgacaacca cagtgccaac cgtttcgccc aaagcactca cgcttacgaa
     2941 aacggcaagc accacaactt atggtgctgc aaatcagcaa attacattta cctatatagt
     3001 taagaatagc ggaactgttc aattaggtcc ggcacaattc accattgccg atac
//
LOCUS       DAOQJE010000402         3051 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_21505_length_3051_cov_5.759680, whole genome shotgun sequence.
ACCESSION   DAOQJE010000402 DAOQJE010000000
VERSION     DAOQJE010000402.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3051)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3051)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3051
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21505_length_3051_cov_5.759680"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..862
                     /locus_tag="PLT08_10815"
     CDS             <1..862
                     /locus_tag="PLT08_10815"
                     /inference="COORDINATES: protein motif:HMM:NF019581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HNE05001.1"
                     /translation="KLRVCKNIPVESVEQANDIGLRTGFGDEMLWIGSVKAFMDGALG
                     PRTAAMLEPYENESGNKGILNMDGEELFEHARKAADVGLSMTVHAIGDKANHEVLNAF
                     EQLRNYETQNHLPHLRHRIEHVQVIHPDDAPRLAKLNVIASMQPIHATSDMFAADRYW
                     GKRAELSYAWRTQLNYGAILAFGSDAPVESPNPFWGLHAAVTRQRADGSPSAEGWRAE
                     QKLNLAEALSAYTFGAAYTANAENRLGKLAENYHADLIVLDEDIFKIDPSTLLTMSPS
                     AVMIDGEWVA"
     gene            906..1928
                     /locus_tag="PLT08_10820"
     CDS             906..1928
                     /locus_tag="PLT08_10820"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNE05002.1"
                     /translation="MESKSPKLSPEMLVPRLGEYLVQKGLISEIDLQRALNHQQDVTY
                     KGGQSLLGQALIELKLIDKDTLDQVITEQIIQLRSALQSANRNLEQRVRERTSDLNEA
                     LNRLSELSQMKANFVSNISHELRTPLTHIKGYIELMLAESLGPISDEQRHALTVSQRS
                     TGKLEDMIEDLIMFSLASRGEMSMKLEGVDIRRLVSLVTKTAMRKAEDRGVKLEVLVK
                     DDTPYVQADGEKISWVVGQLIDNGIKFTPSGGSVTVAIEEEGKNLVQVIVRDTGVGIP
                     QDRMKEIFEPFHQLDGSSTRRYGGTGLGLSLVRQIVEGHGSLLDVKSTEGKGTTFKFP
                     LLAMRD"
     gene            1932..>3051
                     /locus_tag="PLT08_10825"
     CDS             1932..>3051
                     /locus_tag="PLT08_10825"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing sensor histidine kinase"
                     /protein_id="HNE05003.1"
                     /translation="MDKETLSDLHRIFQDVQAKPDWRSALDALFISMRRSFVFDNVAV
                     YLSERQTRGLDVVYARAIGRGKTAEADAAWGESVANDVITNEHMVIHGPAKGSNHRDR
                     MSQTFVLGIPLYVGEKLRGALLFVRFGGPEYSDIHIHVASLQAIWAAALIERKELQEA
                     REELDSVQRQMRLQDDFVSTISHELRTPLGFIKGYSTSLLRQDTQWDDATQREFLSII
                     DEETDRLAKLIEDMLESARLQSKTIQFKFSPIRLDAVVRDVAARVKTHHPNLVIEMNM
                     DVLPPVLGDGVRLSQVFENLFSNAIKYAPGSKLIVGGSHQKKKVRISFADEGDGIPEE
                     FLPFLFERFYRVPGERTVTGTGLGLYICKQIVMAHHGNI"
BASE COUNT          772 a          681 c          818 g          780 t
ORIGIN      
        1 aaaattacgt gtatgtaaga acatccccgt tgagagtgtg gagcaggcga atgatatcgg
       61 cttgcgtacc ggttttggcg atgagatgtt atggatcgga tcggtcaaag cttttatgga
      121 tggtgcactg ggtccgcgca cggctgccat gcttgagcca tacgagaatg agtcgggcaa
      181 taaaggcatt ctcaatatgg atggcgagga actttttgaa cacgcccgca aagctgccga
      241 tgttggtttg agcatgaccg tccatgcgat cggtgacaaa gcgaatcatg aagtattgaa
      301 tgcttttgag cagttgcgca attacgaaac gcaaaatcat ttgccgcatc tgcgtcatcg
      361 cattgagcat gtgcaggtca ttcatcccga tgatgcgccg cgcctcgcca agctcaatgt
      421 catcgcatcc atgcagccca ttcacgccac ctccgatatg tttgctgccg atcgctattg
      481 gggcaaacgc gccgaacttt cttacgcctg gcggactcaa ctcaactatg gcgcgatcct
      541 cgcatttgga tcggatgcac ccgtggagtc accgaacccg ttctggggat tgcatgccgc
      601 agtcacccgc caacgggcag acggctctcc ctccgctgag ggctggcgcg ccgaacaaaa
      661 attgaatctc gccgaagcct tgtccgctta tacctttggt gcggcgtaca ctgccaatgc
      721 cgagaatcgc cttggtaaac tggctgaaaa ctatcacgca gaccttattg ttttggacga
      781 agatattttt aaaattgacc cctcaaccct gttgacaatg tcgccttctg ctgtcatgat
      841 agatggggag tgggtcgctt agagatttgg cacgaaaaaa aataaagaaa ttcggacggt
      901 ttcggatgga atcaaagtcg ccaaaattat caccggagat gctcgttccg cgtttggggg
      961 agtatctcgt ccaaaaaggg ttgatcagcg aaatagacct tcaacgtgcg ttgaaccatc
     1021 agcaggatgt aacctataaa ggcgggcaat ctcttttggg tcaggcattg atcgaactca
     1081 aactcatcga taaagacaca ctggatcagg tcattacgga gcagatcatt caattgcgct
     1141 ctgccctaca atcggcaaac cgcaacttgg agcagcgcgt ccgtgaacgc acctcagacc
     1201 tgaatgaggc attaaatcgc ctgtctgagc ttagccagat gaaggcaaat tttgtttcaa
     1261 acatctcgca tgaattaaga actccgctta cgcacatcaa aggctatatt gaattgatgt
     1321 tggcagagtc gctgggaccc atctccgatg aacagcgcca tgcattgacg gtcagtcagc
     1381 gttctacggg gaaactggaa gacatgatcg aagatctcat catgttctct ttggcatctc
     1441 gtggcgaaat gagtatgaaa cttgaaggcg tggatattcg ccggctagtt tcattggtta
     1501 caaagaccgc catgcgcaaa gcagaagacc gtggtgtgaa gttggaagta ttggttaagg
     1561 atgatacgcc atatgttcaa gcagatggcg agaagatcag ctgggtagta gggcaattaa
     1621 tcgataacgg aatcaagttc accccgtcgg gcggaagcgt aacagttgct attgaagaag
     1681 aaggtaaaaa tctagttcag gtcattgttc gcgatacagg ggtcggtatt ccacaggacc
     1741 gtatgaaaga gatcttcgag ccgttccatc aattggatgg ctcttcgacg cgacgctacg
     1801 gcggcacagg attggggtta tcactggtac gtcaaattgt ggaagggcat ggttctttgc
     1861 tggacgtgaa atcaacggag ggcaagggga cgacgtttaa attccctctg cttgccatgc
     1921 gagattaacc tatggataaa gagacgcttt ctgaccttca tcgaatcttt caagatgttc
     1981 aagcaaaacc agactggcgc tcagcactgg acgcgttgtt tatctccatg cgtcgttctt
     2041 ttgtgtttga taatgttgcc gtctatttaa gtgaacgcca aacaagaggg ttagatgttg
     2101 tgtatgcgcg tgccattggg cgtggtaaaa ccgcagaagc agacgctgct tggggcgaaa
     2161 gcgtggcaaa tgacgttatc acgaatgagc atatggttat tcatggaccc gcaaaaggct
     2221 cgaaccatcg tgaccgcatg tctcaaacct ttgtgttggg gattccttta tatgtgggcg
     2281 aaaaattaag aggcgctctt ttgtttgtac gctttggtgg gcctgaatat tctgatatcc
     2341 atattcatgt cgcttcgcta caggcgattt gggcggcggc gttaattgag cgaaaagaat
     2401 tgcaagaggc gcgcgaagaa ctggactctg ttcagcgcca gatgcgtttg caagatgatt
     2461 ttgtctctac gatttcgcac gagttacgta cgccgcttgg gtttattaag gggtatagca
     2521 ctagcttgtt acggcaagac acccaatggg acgacgccac tcagcgtgag ttcttgtcga
     2581 tcattgatga agagacggac cgtctggcaa aactgatcga agatatgctt gagtccgctc
     2641 gcttgcagag taagacgatc caatttaagt tttctcctat tcgcttggat gctgttgtcc
     2701 gcgatgtggc agcgcgagtc aagacgcacc acccgaattt agtgattgaa atgaacatgg
     2761 atgtcctgcc gccagtcctt ggcgatggag tacgtctttc gcaggtgttt gagaatcttt
     2821 ttagtaatgc cattaagtat gcgcccggct caaagctcat tgttggtgga agccatcaaa
     2881 agaagaaggt gcggatctca tttgctgatg aaggcgatgg gattccagag gaattcctac
     2941 cattcttgtt tgagcgattt tatcgtgtgc ccggtgaacg cacggtgacc gggacgggtt
     3001 tagggctgta tatttgcaag caaattgtta tggcacatca tggtaatatt t
//
LOCUS       DAOQJE010000403         3049 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_21526_length_3049_cov_5.964930, whole genome shotgun sequence.
ACCESSION   DAOQJE010000403 DAOQJE010000000
VERSION     DAOQJE010000403.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3049)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3049)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3049
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21526_length_3049_cov_5.964930"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            51..755
                     /locus_tag="PLT08_10830"
                     /pseudo
     CDS             51..755
                     /locus_tag="PLT08_10830"
                     /inference="COORDINATES: protein motif:HMM:TIGR03696.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
     gene            795..1142
                     /locus_tag="PLT08_10835"
     CDS             795..1142
                     /locus_tag="PLT08_10835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE05004.1"
                     /translation="MNLFVKALLLILAVGVGSIATLIGFRRLRLSDENLTVFQKMKKS
                     IAAGFMFSVAILSFFSAIPIFLRSNIHLDFDVNKIFPLFSCIFIPCLIIISIGVFIQI
                     SYLEFLIHSGKKK"
     gene            1427..2182
                     /locus_tag="PLT08_10840"
     CDS             1427..2182
                     /locus_tag="PLT08_10840"
                     /inference="COORDINATES: protein motif:HMM:TIGR03696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
                     /protein_id="HNE05005.1"
                     /translation="MMFYNARWYDPALGRFAQAYTIIPEGQGVQAYDRFAYTSNNPLR
                     YTDPTGHRNCEEDGYCPENWGSPAGWANPFNYPILSGGVVFNIKSIPGAEVALVAHFN
                     WGAIKDAVTENDLSHLEDADFSLNVEATITGGFSEELDGEIFFEGSNAPTVKDKSGLS
                     LISLKNVPVNVGSCIPTGVCGKVTTEIDLKNKTVNSVGYQAGIGQGVDLSVSVFTLSF
                     PVFTGNIFTNKSKGPSWDDFMDEVVKDLKEYGE"
     gene            2186..2713
                     /locus_tag="PLT08_10845"
     CDS             2186..2713
                     /locus_tag="PLT08_10845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE05006.1"
                     /translation="MFLIQKGILPTIIGIVSFLLPAWVLFLDTKYILENLDTRIPEIL
                     GNSTKSIWLIVAMLYFFTMFFPIYFGMLLASIFPTIEIRRDGINFKYWEFLTCKAKWD
                     EIESIIYYPNNYIVLRIDKRGLPMLNGLYFNSLQSKFLKSQLPILVLSPGLEKRDEVI
                     REILKNSSPRIVQKK"
     gene            2827..>3049
                     /locus_tag="PLT08_10850"
     CDS             2827..>3049
                     /locus_tag="PLT08_10850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE05007.1"
                     /translation="MTTLFFGNHYEITNPGAGQIVTKYSFAGATRIAQRKYTIPQSMS
                     VEYFLGDHLGSTSITTDTAGAKVSEMRYKP"
BASE COUNT          866 a          607 c          681 g          895 t
ORIGIN      
        1 ttgggttgat gttctagtgc gaagtctccc gtagggataa tgtccgctgg tatgatcccg
       61 cattggggag gttctcatcg ccggatacga tcatccccga aagtcaaggc acgcaggcgt
      121 gggatcgctt tgcgtatgtc aacaactcac ccattaattt gactgatcct acaggacatt
      181 ggagttttag ctttagcaca agtagcctta tacaaacttt aagtcaagga gtagatcctg
      241 catttgcggc ttttgtttat gatggaactg catcctattt agatgcaggt gcttggatac
      301 tagacgcagg agccgtagca atatctgcag acataacaat tgctgggaca gcagttgcag
      361 gcccagcggg aacgctagta ggggctgctg ctggtgaatt tgttgcagcg ccacttgtcc
      421 ttacaggaaa tattattgcg tctgttgcgt cagcatcttc aatagcatca gatatactca
      481 caggcgacac ctctattgaa tattctggag aaataactga aagttctttt acgatccatt
      541 ctcaaggcgt acttggcccg gacactcaag tggcagaagc atcttttact gcagggtgga
      601 caataccaac atcatacgcg tctctaacag tgcagtctgc tgtcgttgct tacgacgcta
      661 actctgcatt gcaaacattc gatttgccac acattgactt gtcggaatat ataaaaccat
      721 atcaatgtga tgtttttgta aaacttcaat attaattgca aaccacatac ttatgtaaat
      781 gttatggaga aactatgaat ctgtttgtta aagcactact attgatactt gctgtcgggg
      841 taggctcaat tgccactctg attggatttc gaagattaag attatctgat gagaatttga
      901 cggtctttca aaaaatgaaa aaatctatag cggctggttt tatgtttagc gtagctattc
      961 tatctttttt tagtgctatc ccaatctttt tacggagcaa tattcacctt gattttgatg
     1021 tgaataaaat atttccactc ttttcttgta tcttcattcc atgtctgatt attatctcaa
     1081 ttggggtgtt catacagata agctatctag aattccttat tcacagcggc aagaagaagt
     1141 agtggtagta gttcaatata aaggttggtc ttgggtttcc taaaaaagca ccctccgagt
     1201 ccacctcagg ctcggcgggt gtgtaaggtg aaatcaccgc tgtgcaaaaa tacaccgccc
     1261 tgcaaaagat ggaagtgaat gatcttgagt gaccatctcg acttgatcac cagcgccacg
     1321 gacaaacttg gctctaaact ctcggagatg agatacaaac cgtttggcga agtgcgctat
     1381 acatggaagg cgagtcagac caccacaccg gcgcgagggc tttgggttga tgttctacaa
     1441 tgcaaggtgg tacgacccgg ccttgggacg tttcgcccaa gcgtacacga tcattcctga
     1501 aggtcaaggt gtgcaagcct atgatcgttt tgcatatacc agcaacaatc ctttgcgata
     1561 tactgaccca acaggacata gaaactgcga ggaggatgga tattgtccgg aaaattgggg
     1621 atctccagcc ggatgggcta atcctttcaa ttatcctatt ttatctggag gtgtagtatt
     1681 taatataaag tctatacctg gtgccgaggt tgccttggtt gctcacttta attggggggc
     1741 aattaaagat gctgtcacag agaatgacct aagccattta gaagatgccg attttagttt
     1801 gaatgttgag gctactatca ctggaggttt ttcagaagaa ttggatggtg aaatcttctt
     1861 tgaggggagt aatgcgccaa cagtaaaaga taaaagtgga ttgtctctga tttctttaaa
     1921 aaatgttcct gtaaacgttg gatcttgcat tcctactggc gtgtgtggaa aagtgactac
     1981 agaaattgat cttaaaaaca aaaccgttaa ctctgtcgga tatcaggctg gcatcggtca
     2041 aggtgtagat ttaagtgtta gtgtatttac actgtcattc ccggttttta ctggaaacat
     2101 ttttacaaac aaatctaaag gacctagttg ggatgatttt atggatgaag tggttaaaga
     2161 tttgaaggaa tatggagagt aaaacatgtt tttgattcaa aaaggtattc tacccactat
     2221 aataggcata gtaagttttt tgttacctgc ttgggtatta tttctagaca caaaatatat
     2281 tcttgaaaac ctagatacgc gaattcctga aatactgggt aactccacaa agtctatctg
     2341 gcttatagtg gcaatgctgt atttttttac aatgtttttc ccgatatatt ttggtatgtt
     2401 acttgctagt attttcccta cgattgaaat cagacgtgac ggcataaact ttaagtactg
     2461 ggaatttctt acatgcaaag ccaaatggga tgaaatcgaa agtattatat attacccaaa
     2521 taactatatt gttctgcgaa ttgataagcg tggcttgccg atgcttaatg gtttgtattt
     2581 taatagtctg caatctaaat ttcttaagag ccaattacct attctagtct tatctcctgg
     2641 gctcgaaaag cgagatgagg ttataagaga aatcctcaag aattcctccc caagaatagt
     2701 gcagaaaaaa tagatgcatt taaatacgtc cgtcattgca tagaattaac tgcaaccatc
     2761 accgataagt tcaagcagtc gacatatgac ggcgactaca ggcgcgtgaa gagcgtggaa
     2821 aacagggtga ccactctctt ctttggcaac cattatgaga tcaccaatcc cggcgcaggg
     2881 cagatcgtca ccaaatattc ctttgccggg gcgacgcgga tcgcccagcg caagtacacc
     2941 atcccgcaga gcatgagcgt ggaatacttc ctgggtgacc atttgggttc cacatccatc
     3001 accaccgata cagcaggcgc gaaggtctcg gagatgagat acaaaccgt
//
LOCUS       DAOQJE010000404         3044 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_21592_length_3044_cov_6.429910, whole genome shotgun sequence.
ACCESSION   DAOQJE010000404 DAOQJE010000000
VERSION     DAOQJE010000404.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3044)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3044)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3044
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21592_length_3044_cov_6.429910"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1186)
                     /gene="hisS"
                     /locus_tag="PLT08_10855"
     CDS             complement(<1..1186)
                     /gene="hisS"
                     /locus_tag="PLT08_10855"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: protein motif:HMM:TIGR00442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004821 - histidine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006427 - histidyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HNE05008.1"
                     /translation="MANKIQAVKGTREFYPEQMALRNFIYSKVRSAAHAFGYQEWDAP
                     FIETIDLYAAKSGEELVKKQSFTFEDRGGDFVTLRPELTPSLARMIAAKQGELTFPVR
                     WWSFGPFWRYEQPQKGRSREFFQWNVDMLGVNSPEADAELIAVGATFLRSVGLSPERA
                     LIYVNNRRLMDSEFDALGISAEKRLDVSNLVDRRTKMEPAKWDAYALDLGLNQTQLEG
                     LKSILGNFELWKKSDELVRLFATLEALGVKEYVKFDPNIMRGLLYYTGTVFEAFDVTG
                     SLKRAIFGGGRYDNLLADVGGQPLSGVGFAMGDVVIGIILQEAGLLPEFIPSPAQVLV
                     TVFDEKLWMQSFALAAELRAAGLNVMVSPEPTKLPKQFKFADKMKMKIALVIGPDEAA
                     QGL"
     gene            1309..2109
                     /locus_tag="PLT08_10860"
     CDS             1309..2109
                     /locus_tag="PLT08_10860"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013315.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNE05009.1"
                     /translation="MKIDYQEPSKDLLMRIDIHEKYGSANIDVWTNDLLKPQAGMSIL
                     DVGCGAGKLSFLFDDYTKRQAKITGGDFSEELLDKARAKNKERGSNIDFQFLDFNEKF
                     KFEDNTFDLCTSAFAIYYASDLKFTFGEAHRVLKPGGRLFVSGPLPENKQMFYDIIKE
                     ATNATIPPMPGSSRFKGEIFNTIDGIFAKTELHKFENHLTFPEVAPFIDYVRASLSED
                     RRLWTSMFNGKDEYEALIGKITAVAQKWLDRDGKLVMTKVVGGILATK"
     gene            2128..2790
                     /locus_tag="PLT08_10865"
     CDS             2128..2790
                     /locus_tag="PLT08_10865"
                     /inference="COORDINATES: protein motif:HMM:NF014625.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HNE05010.1"
                     /translation="MRVAPTGFNMNFFGKRVLFIGAHPDDIELGCGALLHHIVKNTDA
                     SVLCVTLSDNQKNPDLKKVKNEHLNAMQVLGVPSDNVVLGPFTTRVFPDSRQEILEYF
                     LKLRKEFKPDLIFTHSKQDVHQDHNTMTDEALRAFRGITVLGFDVVRSSYGFFPHFLV
                     EVTEEDVNKKIEALSQYDTYQDRYYFNAELTRSIMVRHGALAERPFAEGFDILRIVGK
                     FE"
     gene            complement(2825..>3044)
                     /locus_tag="PLT08_10870"
     CDS             complement(2825..>3044)
                     /locus_tag="PLT08_10870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE05011.1"
                     /translation="FIYKTEAGDPATMAYAFLSHLHHQYPRLDTHIENIQVNDPHLPG
                     FQKMGYVESFRRIEMWRGNPPSSIMPSK"
BASE COUNT          837 a          795 c          666 g          746 t
ORIGIN      
        1 ccaagccttg cgccgcctca tctggaccaa taaccagtgc aattttcatc ttcatcttgt
       61 ctgcaaactt aaactgcttg ggcagttttg tcggctctgg cgagaccata acattgagtc
      121 ccgccgcacg taattctgcc gcaagcgcaa aagactgcat ccacaatttc tcgtcaaaga
      181 ccgttaccaa cacctgtgcc gggctgggaa tgaattcagg cagcagaccc gcctcttgca
      241 aaataatgcc aatgaccaca tcgcccatgg caaagcctac gccagaaaga ggttgcccac
      301 ccacatcggc aagcagattg tcgtaacgcc cgccgccaaa gatggcgcgt ttgagtgaac
      361 cggtcacgtc gaaggcttca aagacggtgc cagtgtaata gagaagtccg cgcatgatgt
      421 tcgggtcaaa tttgacatat tctttgacac ccaatgcttc cagtgtcgca aagagacgga
      481 ccaactcatc actcttcttc cacagctcaa aattaccaag aatagacttc aagccttcta
      541 gttgagtttg attcaagcca aggtcaaggg cgtacgcatc ccatttggcg ggttccatct
      601 ttgtacggcg atccaccaaa ttagaaacat cgaggcgttt ctcagcagaa atgcctaaag
      661 cgtcgaattc agaatccatc aggcggcgat tattcacata gatcagagcc cgctccgggc
      721 tgagtccgac cgagcgcagg aacgttgctc cgacggcgat cagctctgca tccgcttctg
      781 gcgagttgac tcccaacata tccacattcc attgaaagaa ctcacgcgag cgcccctttt
      841 gaggctgttc ataccgccaa aacggaccga acgaccacca acgcaccggg aaagttaact
      901 ccccttgttt tgctgcaatc atacgtgcca aagatggagt cagctcgggg cgcaaggtca
      961 caaaatcgcc accacggtct tcaaacgtga aagactgctt cttaaccaac tcctcgcccg
     1021 atttcgccgc atacagatca atcgtctcga taaatggcgc atcccattcc tgataaccaa
     1081 aagcatgcgc cgcagaacga accttactgt agataaagtt tcgtaacgcc atctgctctg
     1141 ggtaaaattc tcttgtacct tttactgcct gaatcttgtt tgccatgtat actccaaatg
     1201 ccacgcattt taacatgact cattccagga ttaatcgtaa ataagcattt caaaattcac
     1261 ccactgaatt ttggtataat cccaacatca acttcaagga gagatgatat gaagatcgac
     1321 tatcaggaac ccagcaaaga ccttttaatg cgaattgata ttcatgaaaa atatggctct
     1381 gccaacatcg atgtttggac caacgacctg ctcaaacctc aagcaggcat gagcattctc
     1441 gatgtaggtt gcggtgccgg aaagctcagc tttttgttcg acgattacac caagcgccag
     1501 gcgaagatca ccggcggtga cttctctgaa gaactgctcg acaaagcgcg cgccaaaaat
     1561 aaagaacgcg gctcgaacat tgattttcaa tttctagact ttaacgaaaa gttcaagttt
     1621 gaagataata ccttcgacct ctgcaccagc gcatttgcca tctactacgc ctcagacctg
     1681 aaatttacat ttggtgaagc gcaccgtgtg cttaagcccg gcggacggct ttttgtctcc
     1741 ggtccgctgc ctgaaaacaa gcagatgttc tacgacatca tcaaggaagc caccaacgcg
     1801 accattccgc ctatgcccgg ctcctctcga ttcaagggcg aaatcttcaa caccattgac
     1861 ggcatcttcg ccaagactga gctgcacaag ttcgaaaatc acctcacctt ccccgaagtc
     1921 gcgccgttca ttgactacgt ccgcgcttcg ttgagcgaag accgccgttt gtggacttcc
     1981 atgttcaacg gcaaggatga gtacgaagcg ttgatcggca agatcaccgc tgtggctcaa
     2041 aaatggctcg accgtgacgg caaattggtg atgaccaaag tcgttggcgg cattctggcg
     2101 acaaaataaa ctttatgtag gggcgacttg cgagtcgccc ctacgggttt caatatgaac
     2161 ttcttcggca aacgagtcct ctttattggc gcgcacccag acgacatcga attgggatgt
     2221 ggggcgcttt tgcatcatat cgttaagaat acagatgcaa gtgttttatg cgtcaccctt
     2281 tctgataatc aaaaaaaccc tgacctaaaa aaggtcaaga atgaacatct taacgcaatg
     2341 caagtactgg gtgtgccttc agataatgta gtattaggac ccttcaccac acgcgtcttc
     2401 cccgattcgc ggcaggaaat tcttgaatat tttctaaaac tgcgcaaaga attcaagcct
     2461 gatctaattt ttacccattc caaacaagat gtacaccaag atcacaacac aatgaccgat
     2521 gaagccttgc gcgcctttcg aggtattact gtattgggtt ttgatgtggt tcgttcatct
     2581 tatggtttct tcccgcactt tctcgtggaa gtcactgaag aagatgtaaa caaaaaaatc
     2641 gaagccttat cccaatacga tacctaccaa gaccgctact acttcaacgc cgagctcacc
     2701 cgctctatca tggtccgcca tggggcgttg gcggagcgtc cgtttgcgga ggggtttgat
     2761 attttgagga ttgtagggaa gttcgaataa tataaaaaat aaaacaggct ttcagaagcc
     2821 tgttttattt tgaaggcata atcgatgacg gaggattacc ccgccacatt tcgatgcggc
     2881 gaaaggattc aacatatccc attttctgaa agcccggtaa atgtgggtcg ttgacttgaa
     2941 tattttcaat atgcgtatcc aaccgtggat attgatgatg cagatgcgat aaaaaggcgt
     3001 atgccattgt agctgggtca cccgcttcgg tcttgtaaat aaaa
//
LOCUS       DAOQJE010000405         3041 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_21627_length_3041_cov_5.056263, whole genome shotgun sequence.
ACCESSION   DAOQJE010000405 DAOQJE010000000
VERSION     DAOQJE010000405.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3041)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3041)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3041
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21627_length_3041_cov_5.056263"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1441)
                     /locus_tag="PLT08_10875"
     CDS             complement(<1..1441)
                     /locus_tag="PLT08_10875"
                     /inference="COORDINATES: protein motif:HMM:NF015722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HNE05012.1"
                     /translation="MKRPVIFLLLWMLALQACGGGTATPTAEPVSETTAPPTATLTEV
                     PTEAPTEAAIVPVDLVGPPMEVGSKYVFVDGTTLIAVPGGTFLMGDINSADNPEREIT
                     VSSFWLYSTKVTNGQYALCVSSGKCSPPDPVNAEHFGNPAFTNFPVTGVTYTQASDYC
                     SFVKGHLPTDAQWEKAARGPDGNIFPWGNQAPECSLLNFGFCEGKTTYINQYPEGKSF
                     YEAWDMSGNVREWVYDWYDPNYNVENPIADPQGPDTGTERSVRSNSYQDSANAAIAAN
                     RFSLDPSLNQPDLGFRCAVNEENPAKLFAPWCQSIAFAGIAPDGTDTGCTPIVVCNDV
                     SLSISSECGSDNVPFSVVSFQLNQHPPNDWTFDVPGCQNLGSTPLKAKFLCEVPGSGG
                     PATVTGSCQDTVSCASACPAHYVDTGEGCVWDGSSTGSTDCLPGSTFDPLTQCCTVTD
                     PNQTAQAFCPVGYFSVNGVCYQVPHGVPEH"
     gene            1878..>3041
                     /locus_tag="PLT08_10880"
     CDS             1878..>3041
                     /locus_tag="PLT08_10880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE05013.1"
                     /translation="MPIPYAVFITIFYFIACLGMGSIVFISIPTLSAKISASPLTVLA
                     TKFLLGQGLLANLWVFMALAGHFSPFFVKGFVGILFLAGVWLNYKSMFPISNQLAKIG
                     NEIKLETLSWKVTIGATLVICLIWLPFWFPLQTSGSRYYMAIAKVISASEHLKVLPGY
                     EEVASLGMQGEVQFAALMTLGSPESTQFFSWLTFVAGCVLLLALGRQVGLKRRGQWIT
                     LTIVFTSSAVILLSGSGKTDLYGAAFAFAAYYWAFRLDEENRTQASILIGLFSGMAVM
                     AKLNYAVNFLPSLALILIWKIFLLPGLKITKEQLLPFLIMGIGGLFIAVQMPIKNQIL
                     FGDPLAAFHSGDISSQVWYGAETIRRVYTLLPFSLTFGAFWGQGGGVSPLILAF"
BASE COUNT          733 a          645 c          776 g          887 t
ORIGIN      
        1 tatgttcagg aacaccgtgc ggaacttggt aacaaacacc gttcacagag aagtaaccta
       61 ctgggcaaaa agcttgggcg gtttgattcg ggtctgtcac tgtacagcac tgcgttagtg
      121 ggtcgaaagt gctaccgggg agacagtcgg tagagcccgt agaactgcca tcccaaacac
      181 agccttcgcc ggtatctaca taatgggctg ggcaggctga ggcgcaggaa actgtgtctt
      241 ggcaagagcc tgttactgta gctggccccc cagaaccagg aacttcgcac aagaattttg
      301 ccttcagggg ggtactgccc aagttttggc agccaggcac gtcaaacgtc cagtcgttgg
      361 gtgggtgttg attcaactgg aatgagacta ctgaaaatgg aacattatca ctgccacact
      421 ctgaactgat gcttagcgat acgtcgttac aaacaacgat tggagtacag cctgtgtcgg
      481 tgccatcggg ggcgataccg gcaaaagcaa ttgattgaca ccatggagca aaaagcttgg
      541 ctgggttttc ctcgtttaca gcgcagcgaa agcccaggtc tggctgattc agggatgggt
      601 ctagcgagaa gcggtttgcg gcaatggcgg cattagcgct atcttgataa ctattgctgc
      661 gaaccgagcg ctccgtgcca gtatccggtc cttgagggtc tgcgatggga ttctctacgt
      721 tgtaatttgg gtcataccaa tcgtagaccc attcacgaac gttgcctgac atatcccaag
      781 cttcataaaa acttttgcct tcaggatatt gattgatata cgttgtttta ccttcgcaaa
      841 acccaaagtt caagaggcta cattcaggcg cctgattgcc ccaaggaaaa atattgccat
      901 cgggtccgcg tgcggctttc tcccattgag catcagtagg cagatgacct ttgacgaaag
      961 aacagtaatc acttgcctga gtgtaggtga cgccagtaac agggaagttc gtgaaagctg
     1021 ggttgccaaa gtgttcggca ttgacggggt ctggcgggga gcatttgcct gaggagacac
     1081 acaaggcata ttgaccattg gtgaccttgg tgctgtagag ccaaaaacta ctgacggtga
     1141 tctcgcgttc ggggttatca gcagagttga tatcgcccat gaggaaggtt ccgccaggaa
     1201 ctgcaatgag ggtagtgcca tcgacgaata catattttga accgacttcc atcggcggac
     1261 ctacgaggtc aacagggacg atggctgctt cggtaggcgc ttcagttggg acctcagtga
     1321 gggttgctgt tggcggggct gttgtttctg agacaggttc tgcagttggt gtggctgttc
     1381 cgccaccgca ggcttgtagt gcaagcatcc ataacaataa aaatattact gggcgtttca
     1441 tgctttctct cctttatata ttctttttca gcttgaggga atcatagtag aagctttgca
     1501 gaatgaaaat ctgtaaaggt tggctgtttg attggaaatt ggttgggaag cctactaact
     1561 aaagtaagtg gcgtttctgc ggcttgtact tcaaaatgaa atcactcgct gaatggtaaa
     1621 attttcaaat tatgcaaaag gaaatcaggc tcagccgaaa atttgtttct gttgttatca
     1681 aagttttatt gacaatatgg atgcttgccc ttttcgcgct ccaagttttg ttgtacccac
     1741 cggcgcctgt ttttcaggtc atcaagaaaa tgggaggcag tgactctttc acctctctgc
     1801 aaagtaaggt actgccctat tttcagactt ctgacctcaa catagagttt gccattcaat
     1861 ttaacgactg attttttatg ccgattcctt atgctgtctt tataacaatc ttttatttca
     1921 ttgcctgctt aggaatggga agtattgttt ttatttctat ccctacactc tctgccaaaa
     1981 tttcggcatc gccattaact gttcttgcta ccaagtttct gcttgggcaa gggcttttgg
     2041 caaatctatg ggtttttatg gcgcttgccg ggcatttttc cccattcttt gtaaaaggtt
     2101 tcgttggtat tttgtttctt gcaggagttt ggcttaatta caagtcgatg tttcctataa
     2161 gcaaccaact ggctaagata gggaatgaaa taaagctcga aacgttgagt tggaaggtca
     2221 ccattggcgc gactctcgta atatgtttga tttggctgcc attttggttt ccacttcaaa
     2281 cctctggctc gcgctactat atggcaattg ccaaagtcat ctcggcttcg gagcatctta
     2341 aggttctgcc agggtatgag gaagttgcca gcttgggcat gcaaggtgag gtgcaatttg
     2401 ccgcgttaat gaccttgggc agcccagaaa gcacacagtt ctttagttgg ttgacctttg
     2461 tggcggggtg tgtgctttta ttagctcttg gcaggcaagt gggattaaaa cgccgtggac
     2521 aatggataac gcttaccata gttttcactt cttccgcagt aatcctatta agtggtagcg
     2581 gaaaaacaga cctttatggg gctgcctttg cctttgctgc ttactattgg gctttcaggc
     2641 tggatgaaga gaatcgcact caggcttcta ttcttattgg tctattcagc ggtatggctg
     2701 tgatggcgaa gttaaattat gctgttaact ttctgcccag tctagcgttg atattaatct
     2761 ggaagatatt tctgctacct ggtctaaaaa taaccaaaga gcaactcctt ccattcttga
     2821 ttatggggat aggcggttta tttattgcag tgcaaatgcc gatcaaaaat caaattttat
     2881 ttggagatcc attggcggca tttcatagcg gagatatttc cagccaggtt tggtatggag
     2941 cagagacgat tcgcagggtt tatacattgc ttccattttc actgaccttt ggagcatttt
     3001 gggggcaagg tggcggagta agtcctttaa tcttggcttt c
//
LOCUS       DAOQJE010000406         3039 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_21651_length_3039_cov_5.980228, whole genome shotgun sequence.
ACCESSION   DAOQJE010000406 DAOQJE010000000
VERSION     DAOQJE010000406.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3039)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3039)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3039
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21651_length_3039_cov_5.980228"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(96..629)
                     /gene="def"
                     /locus_tag="PLT08_10885"
     CDS             complement(96..629)
                     /gene="def"
                     /locus_tag="PLT08_10885"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HNE05014.1"
                     /translation="MAIRQIVHLPEPILRKKAKPVTKFDKDLQTLIDDMIETMREAPG
                     VGLAAPQINLPMQLAVVEYAEGEDEEREEGAPEPKKKLYVLINPEIIKVSEEKVLGIE
                     GCLSIPGLVGEVERHEAIQVKALNRHGDPVKIKVDGWMARIFQHEIDHLNGELFTDKA
                     VRVWKPSEEQDEETPLD"
     gene            complement(743..2635)
                     /locus_tag="PLT08_10890"
     CDS             complement(743..2635)
                     /locus_tag="PLT08_10890"
                     /inference="COORDINATES: protein motif:HMM:NF012257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HNE05015.1"
                     /translation="MKFDRITNVIEFKQIVIFLDKMHLFGGLDQNQLSGIANKLVEKE
                     VPAGKEIFKRGEKPDGFYMIFKGKVRVTVPDAEKGERQVAVLYGGDYFGEEALFENRN
                     RSATVRAVEDTTILFLSRQGFDGLLKQYETLKPNFQVSIKSRKLARSTRFKWLGKNEV
                     IYFIARRHKIRLFQALVTPFFSLLGALALIALGFFVSSVIPAAVGVVLVIGVLGWGVW
                     NAIDWSNDYYIVTNQRVVWLEKIIFLHDSREEAPLGTILSVGVETDALGRILKYGNVI
                     VRTFVGKLEFEYVDHPQQAADMIREYWERTKAITTRTQMDVMKDTIKQKLGLPLDKRP
                     QVELSPVVPMDEEQVLKTPLWMLALQNLFQLRLEEGGKIIYHKHWIILLQQGWRPFGL
                     FFLGIILFIWRMVVLYNSPDKAFITFNGGFSFKPDTISVVIPLLLLPVAGWVWYEYTD
                     WKNDTFMVTQDEIFDIDRKPFGTEERRSAQIENILSTSYKRKGFIAYMFNYGTVNITV
                     GGTQMAFEDVMDPAAVQADINRRRAQRIAKKNEDAGSADRERFATWIAAYHQHIGEFN
                     KPITPPANANVSNTNPFAAVSKEKDLDDLPIKDDDDFDMDDFGDDDFDGDDDFGGDGD
                     GGGGDN"
     gene            complement(2625..>3039)
                     /locus_tag="PLT08_10895"
     CDS             complement(2625..>3039)
                     /locus_tag="PLT08_10895"
                     /inference="COORDINATES: protein motif:HMM:NF012787.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="redoxin domain-containing protein"
                     /protein_id="HNE05016.1"
                     /translation="IVNFWSCECPHSERTDKAILSMFVQWQDDVTMLTIASNRSETVE
                     ALKEAAESRRLPNVLHDADCSVANLFDAQTTPHVFVIDRDGILRYRGTVDDVTFRQRK
                     PTRFFLDEAVEALLSGQFPTLTESPAYGCAIVREV"
BASE COUNT          743 a          773 c          735 g          788 t
ORIGIN      
        1 gtggaaggtc agcatccagg cgggtgagac tgcgaaagtt ggtgagggag aggtgtttga
       61 gatacataca gctttcagct gctggcgaat cgagattaat ccagtggtgt ttcttcgtct
      121 tgttcttcgc taggtttcca aacgcggacg gctttatcgg taaaaagctc gccattgaga
      181 tggtcaatct cgtgctggaa gatgcgtgcc atccaaccat ctactttgat tttgacaggg
      241 tcaccgtggc ggttgagcgc cttaacctga atcgcttcgt ggcgctccac ttcgccgacc
      301 aagccgggaa tggagagaca accttcaatg cccaagacct tttcttcaga gactttgatg
      361 atctcagggt tgatgagcac atacagcttt ttcttcggtt cgggcgcgcc ttcttcgcgt
      421 tcttcatctt cgccttcggc gtattccacc acggctaatt gcatgggcag gttgatctgc
      481 ggcgcagcaa gacccacacc cggcgcttcg cgcatggttt cgatcatgtc gtcgatgaga
      541 gtttgaaggt ctttatcgaa ttttgtaacg ggttttgcct tcttccgcaa aatgggttcg
      601 ggcaggtgaa cgatttgtcg tattgccata cttggtcttt tcctttttgt ttcagcgtac
      661 aagtgtaagg gtgaccctta cgattttgtt tttcaatagg gcggcaatga tacttggatt
      721 gtcttttccc cgctagccta cgttagttat ctccaccgcc gccgtccccg tcaccgccaa
      781 aatcatcatc accatcaaaa tcatcgtcgc cgaagtcgtc catatcgaaa tcgtcatcat
      841 ctttgatcgg gaggtcatcc agatcttttt cttttgagac ggcagcaaac ggattcgtgt
      901 tgctaacatt ggcattagca ggcggggtga tcggcttgtt gaactcacca atatgttgat
      961 gataggctgc gatccaagtg gcgaaacgtt cacggtcggc gctgccggca tcttcatttt
     1021 tcttagcaat gcgctgagcc cgccgacggt taatgtcggc ttgcacagcg gcagggtcca
     1081 tcacatcttc aaacgccatt tgagtaccgc ctacggtgat gttaactgtc ccgtaattga
     1141 acatgtaggc aataaacccc ttgcgcttgt aagatgtgct taagatgttc tcgatctgag
     1201 cagagcggcg ttcttctgtg ccgaaaggtt tgcggtcaat atcgaagatc tcgtcctggg
     1261 tgaccatgaa ggtatcgttc ttccagtcgg tgtattcata ccaaacccaa cctgctacag
     1321 gtaataacaa aagtggaatg accacagaga tcgtatcggg cttgaaggaa aagccgccat
     1381 tgaaagtaat aaaggctttg tcgggcgagt tatacaacac caccatacgc caaataaaaa
     1441 gaatgatgcc aaggaaaaag agcccgaatg gacgccagcc ttgctgcaac aaaatgatcc
     1501 aatgcttgtg atagatgatt ttgccaccct cttcaagacg caattgaaag aggttctgaa
     1561 gtgccaacat ccacaatggc gttttgagca cctgttcttc atccatgggc actacaggag
     1621 aaagctccac ttgcggacgt ttatccaacg gcaagcctag cttctgcttg atggtgtctt
     1681 tcatgacatc catttgagtg cgggtggtga tggctttggt acgttcccaa tactcacgga
     1741 tcatgtccgc ggcttgctgg ggatgatcga cgtattcaaa ttccagcttg cctacgaagg
     1801 tacgcacgat cacattgcca tatttcagaa tgcgtcccag tgcatctgtc tcaacgccga
     1861 cggataagat agttcccaat ggtgcttctt cgcggctatc atgcaagaag atgattttct
     1921 ccaaccacac aacacgttga ttggtgacga tgtagtaatc gttgctccag tcaatagcat
     1981 tccacacacc ccagcctaaa acgccgatta ccagcaccac tcccaccgcc gccgggatga
     2041 ccgaacttac gaagaagcct aaagcgatta atgccagcgc tccaagcaaa gaaaagaatg
     2101 gcgtgaccaa tgcttgaaag aggcgaattt tatgtctgcg agcaatgaag taaatgactt
     2161 cgttcttccc caaccactta aagcgtgttg aacgtgcaag tttgcggctc ttaatggaga
     2221 cttgaaaatt tggtttaagg gtttcgtact gtttaagcag cccgtcaaaa ccctgacgtg
     2281 aaagaaatag aatagttgta tcttccaccg cccgaaccgt ggcagaccga ttgcggtttt
     2341 caaacagcgc ttcttcaccg aagtagtcgc cgccgtacag cacagctact tggcgttcac
     2401 ctttttcagc atctggcacg gtcacacgca ctttcccctt aaagatcata taaaagccat
     2461 ctggcttttc gccgcgctta aaaatttcct tcccggcggg aacttctttt tcaacaagct
     2521 tgttggcaat accagaaagc tgattctggt ccaacccgcc gaagaggtgc attttatcga
     2581 ggaagatgac gatttgtttg aattcgatca cattcgtgat tctatcaaac ttcacgcaca
     2641 atggcgcagc cgtaagcagg agattccgtc agcgtgggga actgcccaga gagcaaagcc
     2701 tcaaccgcct cgtccagaaa gaagcgcgtg ggcttgcgct ggcggaaggt cacatcatcc
     2761 accgtgccac gatagcgcag gattccatcc cggtcgatga cgaaaacgtg cggcgtcgtt
     2821 tgcgcgtcaa ataaatttgc gaccgagcaa tccgcatcat gcaagacgtt tggcaggcgg
     2881 cgactttcag cagcttcttt caacgcctca accgtttcgc tgcgattcga cgcgatagtc
     2941 aacattgtca catcatcctg ccattgcacg aacatggata gtatcgcttt gtctgtgcgc
     3001 tcggaatgcg gacattcgca cgaccaaaaa ttgacaatc
//
LOCUS       DAOQJE010000407         3037 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_21682_length_3037_cov_5.569416, whole genome shotgun sequence.
ACCESSION   DAOQJE010000407 DAOQJE010000000
VERSION     DAOQJE010000407.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3037)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3037)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3037
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21682_length_3037_cov_5.569416"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(133..867)
                     /locus_tag="PLT08_10900"
     CDS             complement(133..867)
                     /locus_tag="PLT08_10900"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNE05017.1"
                     /translation="MDLREYELMYQVEDSHWWYEGMSTITRSLLDYWVSAKNLRILDA
                     GCGTGAAMTRYLSDYGSVTGIDLYAEALHFCLKRNAKRLARASVLKLPFASNSFDLIT
                     SFDVLYERAVLDETTALKEFSRILAPNGHVLLRLPAYDWLRGKHDERVHTNRRYTTRQ
                     VAGLLEKSGLHVQHLSYANTILFPLAVAKRLSERFFPPNEDYSELSLNAGMFNNFLKF
                     ILASEEPFITKYGLPFGLSVIAVGRK"
     gene            complement(880..2844)
                     /locus_tag="PLT08_10905"
     CDS             complement(880..2844)
                     /locus_tag="PLT08_10905"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HNE05018.1"
                     /translation="MTERSSKTSGDVNNVRRAIAFVGIMCILISQFLIFSQPVDHTIV
                     FPPYTFLAFIGIVIFSLSWFIKPNAILEKAATWLLFNDRVFWLFVAFALSGVVTFAIV
                     NLTLFARINYIPVVTLWLFSAGAYVYVFWDRSINLSTLVDWFKKNQNELLSVLAVTLL
                     AVVVRFYKLGGIPRILDGDEGLIGLFAQSTTSGTLANPFALWENFGAMYLQLINTSIG
                     LFGVNAFALRLLPAIGGTLAIPSIYLLARWIGGRRIALIAALILAFSHTHIHFSRIVS
                     VAYIHGTWLAPLELYLLLSGLEKRQSWRTALSGIILAIHFSVYVTAQVIAALAFIFML
                     LSLIFYRQWFKERIRQALVFWGAFLTLILPSAYYIFKNPNEFFSRMSANGTFQSGWLQ
                     LTMETTGQSAVEVLFGRFVHAFLSLIYYPAFDFYGSTSPVMSMITSAFFLAGLVIALL
                     RLRKSEYLLLNGYFWGATFSVGIFAIPPSADSYRMLMALPAAFIMAALGLDQILEAFG
                     FGWKNNRSAYIFSASAVLTSLLIFNMWTYYGDFAGKCRFGNDLVGRFASYLGSYAGSV
                     ENEYPIYLLSDTNYFYGSHGSTDFLSQRRAIINVSDPVDTLNPVSGETIIANPDRIPE
                     LEAWARTHPGGQLNYRYDCDKTILLAYQVP"
BASE COUNT         1021 a          692 c          595 g          729 t
ORIGIN      
        1 catcgttgac gacaatgatc tcaaaatcgt ttgtcacctg cttgcaagcg atactagctg
       61 ccgccaccat gcttgcgatt gttccgccat cgttatatgc tgggaaaaca gcactgatac
      121 tggaaatttt gctcattttc gccccactgc aatgacgctc aagccaaatg gcaagccgta
      181 tttcgtaatg aacggctcct cgctagcaag aataaatttc aaaaaattat tgaacattcc
      241 agcattcaaa gaaagctcag aataatcttc attgggagga aaaaacctct cgctgagtct
      301 ttttgctaca gcgagaggaa agagaattgt atttgcatat gaaagatgtt gcacatgcag
      361 tccgctcttt tcgagcaagc cagccacttg tctggttgta taacgacggt tggtatgaac
      421 ccgttcatca tgctttcccc taagccaatc ataggcaggc aagcgcaaga ggacgtgtcc
      481 attcggggca aggatacgag aaaattcttt taaggctgtc gtttcatcca agacagcccg
      541 ttcgtataag acgtcaaagc tggtaattag gtcaaaggaa ttagaggcaa acggcagctt
      601 aagcacagaa gcgcgcgcaa gccgttttgc attccgtttc agacaaaagt gtagagcttc
      661 tgcgtacagg tcaattccag ttactgaccc ataatcagaa agataccggg tcatggcagc
      721 ccccgtgcca caaccagcgt ctaatattct taagttttta gccgataccc aataatcaag
      781 taaagagcga gtaatggtac tcattccttc ataccaccaa tggctgtctt ccacttgata
      841 catcagctca tattcgcgta aatccaaatc tcctctttcc taagggactt gatatgccaa
      901 taaaatggtt ttgtcacaat catagcgata gttcaattgt ccgccaggat gagtacgagc
      961 ccaagcctcc agttcaggaa tacgatctgg attggcaata atggtctcac cagaaacggg
     1021 attcagggta tcgaccgggt cagatacatt gataatcgct cggcgctgac ttaggaaatc
     1081 ggtagaaccg tggcttccat aaaaatagtt tgtatcactc aaaagataaa ttggatattc
     1141 attttccaca cttcctgcat aactacctaa gtaagaagca aaacgcccaa caaggtcatt
     1201 accaaagcga cattttccgg caaagtctcc gtaatacgtc cacatgttaa agataagcaa
     1261 gctggtaaga acagcagatg ctgaaaagat ataagctgag cgattattct tccaaccaaa
     1321 gccaaatgct tcaaggatct ggtctagccc taatgcagcc ataataaaag cggcaggaag
     1381 cgccattaac attcggtatg agtctgcgct ggggggaatc gcaaaaatgc ctaccgagaa
     1441 tgtagccccc cagaaatatc cgttcaaaag caggtattcc gatttccgaa gtcgtaagag
     1501 ggcaatcacc aagccagcca gaaaaaaagc actggttatc atgctcatta ccggtgaggt
     1561 agaaccataa aaatcgaaag ctggataata tattaaagac aagaaggcat ggacaaacct
     1621 tccgaataat acttcaacag cactttgtcc tgtcgtttcc atggtaagtt gaagccagcc
     1681 agattgaaat gtcccatttg cagacatgcg gctaaagaac tcattcgggt ttttgaaaat
     1741 atagtacgcc gatggcagaa taagagtcaa gaaagcaccc caaaacacca atgcttgtct
     1801 gatgcgttcc ttaaaccatt gccgataaaa tatcaacgac aacagcataa aaataaatgc
     1861 aagtgcagca atcacttgtg cagtaacata aaccgagaag tgtattgcaa ggatgatccc
     1921 gctcaatgca gtacgccatg attggcgttt ttctaagcca ctcagaagta aatacaattc
     1981 cagtggagca agccatgtac catgaatgta agccacagaa actattctgc tgaaatgaat
     2041 atgtgtatga gaaaaggcga ggatcaacgc agcaatcaaa gcaatccttc gcccgccaat
     2101 ccagcgagcc aaaagataga tagacgggat tgccagagta ccaccaatag ctggtaaaag
     2161 gcgtagagca aaagcattta ctccaaacaa accaatggaa gtattaatta actgcaaata
     2221 cattgcccca aagttttccc aaagtgcaaa ggggttcgcc aatgttccac tggttgtaga
     2281 ttgagcgaaa agaccaatca acccctcgtc tccgtctaaa atacgtggaa taccacctaa
     2341 cttataaaac ctgacaacaa ctgccagcaa agtaaccgca agaaccgaga ggagttcatt
     2401 ctgatttttc ttaaaccaat caacaagtgt tgataagttt atgctacggt cccagaaaac
     2461 atatacatat gcaccagcac taaatagcca aagggtaact accggaatgt aattaatacg
     2521 tgcaaacaaa gttaaattga caatcgcaaa agtcacgacg ccagacaagg caaaggcaac
     2581 aaaaagccaa aatacccgat cattaaacag cagccaagtt gcagccttct ctaaaatggc
     2641 attaggttta atgaaccaac taagcgaaaa aataactatg ccaataaagg ctaaaaatgt
     2701 atacggcgga aatacaattg tatgatctac cggttgagaa aaaatcaaaa actgactgat
     2761 taaaatgcac atgatcccca caaaggcaat tgcccttcga acattattta catccccact
     2821 cgtcttactg cttcgttcag tcatcacatc ctcctaagat gaattatctt actgacctac
     2881 acataatcga catcaatgcg aaaggacttt cattataccc aaataaagaa gatgtctagc
     2941 tcagtcctta tcatgaattt atcaggttaa caattcttaa aattcatgta taattaagaa
     3001 aacttaagac accttccgct ttgcggtctt ttgcttg
//
LOCUS       DAOQJE010000408         3034 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_21714_length_3034_cov_6.298087, whole genome shotgun sequence.
ACCESSION   DAOQJE010000408 DAOQJE010000000
VERSION     DAOQJE010000408.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3034)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3034)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3034
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21714_length_3034_cov_6.298087"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..740
                     /gene="cysK"
                     /locus_tag="PLT08_10910"
     CDS             <1..740
                     /gene="cysK"
                     /locus_tag="PLT08_10910"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004124 - cysteine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HNE05019.1"
                     /translation="VEPTSGNTGIALAMVAAARGYKLILTMPETMSKERRMLLRAYGA
                     ELVLTPGPDGMGGAIRKAEEIVASDPKKYFLPQQFKNPANPEVHRKTTAEEIWRDTDG
                     KVDYLVAGIGTGGTITGVGEIIKERKPSFKAIAVEPDASPVLSGGAKGPHPIQGIGAG
                     FVPDILNTKIYDEIIRVKADDAFQTARRLAREEGLLVGISSGAATWAALQVANRAENA
                     GKLIVVIIPSFGERYLSTALFADLVDN"
     gene            797..1489
                     /gene="cysE"
                     /locus_tag="PLT08_10915"
     CDS             797..1489
                     /gene="cysE"
                     /locus_tag="PLT08_10915"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009001 - serine O-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HNE05020.1"
                     /translation="MFKTIREDVQSVLDRDPAARNVLEVLLCYPGVHAIWYHRLSHKL
                     WKGGFKLLARWVSQFARGLTGVEIHPGAKIGKNFFIDHGMGVVIGETAEVGHNVTLYH
                     NVTLGGTSSEKVKRHPTLEDNVVVGAGAKVLGAIVIGEGSRIGANAVVVKSAPPNSVI
                     VGVPGQVLLRSHTNKQIDLNHNLLPDTIGDTVAALVAHVNELEKKMNTHFMGSPLHRS
                     DHGVWHGEDFAI"
     gene            1636..2646
                     /locus_tag="PLT08_10920"
     CDS             1636..2646
                     /locus_tag="PLT08_10920"
                     /inference="COORDINATES: protein motif:HMM:TIGR00374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase transmembrane
                     domain-containing protein"
                     /protein_id="HNE05021.1"
                     /translation="MNKFLVFLFLFLGVVVVVFSFGELETILQTLQKAHLGFFLLAVL
                     IQIAWFVSSGRMYQSIFHLLGIHEDVLTLSRIAAAANFINVVAPTAGAGGVALFAAEA
                     KRRGHPAGKATVAAALFLLLDLAAFLIILSLGLIVLVRRNDLDAGEISASLILFTTAA
                     GFTSVLYIGYRSAEKLGNILAKLARGINKVVHLFRKENYLSEERAHEFAHELADGFSG
                     LTEQPSSLIRPVLWGLFEKGLLMLILMCSFLAFEVSFSAGTIIAGFAIAYLFLIVSPT
                     PSGIGIVEGIMPVALSSLNVNWSQGVVITLLYRAVTFWVPLGIGAWAFRTLHKERAEV
                     AE"
     gene            2652..>3034
                     /locus_tag="PLT08_10925"
     CDS             2652..>3034
                     /locus_tag="PLT08_10925"
                     /inference="COORDINATES: protein motif:HMM:NF012492.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HNE05022.1"
                     /translation="MLSALLDFWKRDEETSPNIFAWQTLPPREAQTHPIPDDVPASLR
                     EALSTRGISQLYSHQQSAWIHARAHRNLILATGTASGKTLAYNLPVLAKLIEDSQARA
                     IYLFPTKALTQDQQSSLESFQSSIV"
BASE COUNT          699 a          753 c          749 g          833 t
ORIGIN      
        1 tcgtagaacc cacatctgga aacacaggca ttgccttggc aatggtcgcg gcagcacgtg
       61 gatacaaact catcctcacc atgcccgaaa ccatgagcaa ggagcgccgc atgttgcttc
      121 gtgcatatgg agcagagttg gttcttaccc ccggacccga tggcatgggc ggtgcgattc
      181 gcaaggcgga ggaaattgtc gcctcagacc ccaagaagta ttttctgccg cagcaattta
      241 agaatcctgc caaccctgaa gttcaccgca agaccaccgc ggaagagatc tggcgcgaca
      301 cagacggcaa agtagattat cttgtggctg gcattggcac aggtggaacc atcaccggtg
      361 tgggcgagat catcaaagaa cgcaaacctt ccttcaaagc catcgccgta gagccagatg
      421 cctcccccgt tctttctggc ggcgccaaag gaccgcatcc gattcagggg attggtgcag
      481 ggtttgtgcc cgatattctc aataccaaaa tttatgatga gatcattcgt gtcaaagcag
      541 atgatgcttt tcagaccgct cgccgcttgg cacgtgaaga agggttgctg gtagggattt
      601 cttcaggtgc agcgacatgg gctgcgcttc aggttgccaa ccgcgctgaa aatgcaggca
      661 aattgatcgt ggtcatcatc ccttcgtttg gggaacggta cttgtccacc gctttgtttg
      721 cagacttggt cgataactaa tattttaaag cgctcaatta aaagagcgtg atggttttct
      781 cgtaaagtga gtttcaatgt tcaagacaat tcgtgaagat gttcaatctg tgctggaccg
      841 tgaccccgca gcccgcaatg tgctcgaggt gctattgtgt tacccgggcg tgcatgccat
      901 ttggtatcac cgtctttcac acaagctttg gaaaggcggt ttcaaactgc ttgcacgctg
      961 ggtctctcaa ttcgcacgcg gactgaccgg tgtagagatt caccctggcg caaagatcgg
     1021 taagaacttc tttattgatc acggtatggg cgttgtgatc ggtgagacgg ctgaagtagg
     1081 gcataatgtc accttgtatc acaacgtgac tctgggcggc accagttctg aaaaagtgaa
     1141 acgccacccg acattggaag ataatgtagt cgttggcgct ggtgcaaaag ttttgggcgc
     1201 gatcgttatt ggagaaggca gccgtattgg tgcaaatgct gttgttgtta agtctgcgcc
     1261 gcctaattcg gtcattgttg gggttccggg gcaggtcctt ttgcgaagcc ataccaataa
     1321 acagatcgac ctcaaccaca acttattgcc agataccatc ggtgatacag ttgcggcttt
     1381 ggttgcgcat gtgaacgaat tggaaaagaa aatgaacaca cactttatgg ggtctccttt
     1441 gcatcgatct gatcatggag tatggcacgg cgaagatttt gccatttagg ttttgatctt
     1501 ccgcaacata atagaccgtt actgcgtata aaacacgcgg caacggtcta tttttataac
     1561 tgtcatgaat cttttgctcc cgtacagaaa gggtcaatgt tttgttacaa tgtctgtgta
     1621 ggtatatagg tatacatgaa taaatttctg gttttcttgt tcctttttct gggcgtagtt
     1681 gtggtcgttt tcagctttgg cgagttggag acgattcttc agactctcca aaaagcacac
     1741 cttggctttt ttctattggc agttcttatt cagatcgctt ggtttgtttc cagcgggcgg
     1801 atgtatcaat ccatctttca tctattgggg attcatgaag atgtgctcac tctcagccgc
     1861 attgccgcag cggcgaattt tatcaatgtc gtggcaccca ctgccggcgc gggaggagtc
     1921 gcactctttg ctgccgaagc gaaacggcgt gggcatcctg ctggcaaagc gactgtcgca
     1981 gctgcattgt tcctcctatt agacctggca gctttcttga tcatcctctc acttggcttg
     2041 atcgtgcttg ttcgccgaaa tgacttggat gccggagaga tctcagcctc tctgatttta
     2101 tttacaacag ccgcaggctt tacatctgtt ttatatatcg ggtatcgctc tgctgaaaaa
     2161 ttggggaaca ttcttgcgaa acttgcacgt ggcatcaaca aggtcgtaca tcttttccga
     2221 aaagaaaatt atcttagcga agagcgagcc catgaatttg cacacgaact tgccgatggg
     2281 ttttcagggt taacagaaca accctccagc cttattcgcc cagtgttgtg gggcttgttt
     2341 gaaaaaggtt tgttgatgtt gattcttatg tgctcatttc ttgccttcga agtttcattt
     2401 tcagctggca ccatcattgc tggtttcgcc attgcatacc tctttcttat cgtctcgcct
     2461 accccatctg gcattggtat tgtcgaaggc atcatgccgg ttgccttaag ctctttgaac
     2521 gttaactgga gtcagggcgt ggtcatcaca ctgctctatc gcgcggttac attttgggtg
     2581 ccattgggaa ttggtgcttg ggcatttcgc accttgcata aagaacgcgc tgaggttgcc
     2641 gagtaacgtt catgctctct gctcttctgg atttttggaa gcgggacgaa gaaacctcac
     2701 ccaatatctt tgcctggcaa acgcttccgc cacgtgaagc tcaaacgcat cccattccag
     2761 acgatgtgcc tgcctcgctt cgagaagcct tatctacccg cggcatttcc caactctatt
     2821 cccaccaaca gtcagcgtgg attcatgccc gtgcgcatcg caatttgatc ctcgccaccg
     2881 gcaccgccag cggaaagact ctcgcgtaca acctgcccgt tctcgcaaaa ctgatcgaag
     2941 actctcaagc gcgcgcaata tatctctttc caaccaaagc ccttacccaa gatcaacaat
     3001 caagcctcga gtcttttcaa tcgtcaatcg taaa
//
LOCUS       DAOQJE010000409         3033 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_21726_length_3033_cov_5.067159, whole genome shotgun sequence.
ACCESSION   DAOQJE010000409 DAOQJE010000000
VERSION     DAOQJE010000409.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3033)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3033)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3033
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21726_length_3033_cov_5.067159"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1317)
                     /locus_tag="PLT08_10930"
     CDS             complement(<1..1317)
                     /locus_tag="PLT08_10930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE05023.1"
                     /translation="MPQQSRLYPEFGALFFNILAEYLPPSSKKNKNTIAEYLIDQIKK
                     RGEQVGRGYRANVSAWLNGDIAGRKSLSPDSFLYILKLLAADNRYTLTVDDVESLIEK
                     GRDDYKGILQDKFVQSLRVYSRPLLAPVPHIIRREEIENQIIKELSVEGAHIVILYGP
                     PGRGKKMLMRLATRQRFDILPRRSIVIVGQTVKLLTEWVKLVINKPKVTETNSLRFLM
                     EEFRAKFNGQSVVFTLSDCDELEVLEWLQKYLPAGCKLLVSTHHAAIAENMPNLPVIR
                     IPPFTEKETVEYMQAYVPGQAWTSEEFRQFYRLTMGTPLTLYLAYRHILERGMEFVLD
                     KLSIIPVLDQTEDLVGLHRAILLGFESLAPKLKEAFRKIALLDNYLSYDLLTFQALWQ
                     NSPGELLGRGETREYLLRLCDQAGLIQSVGKDEWEIYPVIKKFAERT"
     gene            1710..2042
                     /locus_tag="PLT08_10935"
     CDS             1710..2042
                     /locus_tag="PLT08_10935"
                     /inference="COORDINATES: protein motif:HMM:NF012464.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HNE05024.1"
                     /translation="MNEINKAAAMYIRYLPGNYKGLKTQLIEITRLAMAEGITITQFY
                     IDEVSLVQSIERPELQQLLKFQTLYVYTKDRFARDAHQANTILTQLASMSVHIHFVAE
                     TLPPHSMG"
     gene            2123..>3033
                     /locus_tag="PLT08_10940"
     CDS             2123..>3033
                     /locus_tag="PLT08_10940"
                     /inference="COORDINATES: protein motif:HMM:NF012464.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HNE05025.1"
                     /translation="MNNNHLETGSAYIRYSSTMQDDSFSLDAQLRQIKIRAASDGVEI
                     VKVYSDPATSAYSKKNRPGLEEMLADAKRGLFKFAYVHKLDRLSRRLEWALEIVKILQ
                     NAGVTLRSVEQKFDLDTPDGKLLFAMLGSLGEFFSDNLSQETGKGKLERATQGYHNNQ
                     VPWGYKSELVGVKKQAVPDPNTQEIVKQMFERYSTGLYYDQEIADWLTDLGHITSRGR
                     PFTKDTVREMLLNKFYLGLTNYNGRHGHKGKRHEKDRVWIKGVHSPLISKELFEKCLK
                     VRASRRRMMNSKQKTFRVYFLAGIITC"
BASE COUNT          882 a          723 c          624 g          804 t
ORIGIN      
        1 agttcgctca gcaaactttt ttattaccgg atatatctcc cattcatcct tcccgactga
       61 ttggattaac ccggcttgat cacataaacg aagaagatac tcccttgttt ctccacgccc
      121 taacagctcg cccggtgaat tctgccaaag tgcttggaaa gttaatagat cataacttag
      181 gtaattatcc aaaagagcaa tctttcgaaa cgcctccttc agtttgggag caagactctc
      241 aaagcccaaa agtattgctc gatgcaaacc taccaaatcc tcagtctgat cgaggacggg
      301 aatgatagac agtttatcta gtacaaattc catgcccctc tccaaaatat gccggtacgc
      361 taaatataat gtcagagggg tacccatggt caacctgtaa aactgcctga attcctcgct
      421 cgtccaggct tgcccaggaa cgtatgcctg catgtactca actgtttctt tttcagtgaa
      481 tggaggaata cgaatgactg gcaggtttgg catattttca gcaattgctg catgatgagt
      541 agagacaagc agtttacaac ccgcaggcag gtatttctgc aaccactcca gaacttccag
      601 ttcatcacaa tcagacaacg taaaaacaac agactgcccg ttgaatttcg ctcggaattc
      661 ctccatcaaa aaacgcaggg aatttgtttc tgttactttt ggtttgttta taaccagctt
      721 tacccactca gttagcagtt tgacagtctg accaacaata acaatactac gtcggggcag
      781 aatatcaaaa cgctgccggg tagctaatcg cattagcatt ttctttcccc tgccaggagg
      841 tccatataga ataactatgt gggcaccttc tacacttaat tctttaataa tctgattctc
      901 aatttcctcg cgccgaatta tatgcgggac tggtgccagt agaggtcgcg aataaacacg
      961 aaggctctga acaaacttgt cttgaagaat ccccttgtaa tcatccctgc ctttttcgat
     1021 caaagattcc acgtcatcaa ccgtcagggt gtaacggtta tcagctgcca gcaatttcaa
     1081 tatatacaaa aaggaatccg gcgaaaggct ttttctgcct gcaatatctc cattgagcca
     1141 ggcagagaca tttgcacgat accctctacc aacctgttct ccccgttttt tgatttgatc
     1201 aattaagtat tctgcaatgg tatttttatt tttctttgaa cttgggggca aatattccgc
     1261 cagaatattg aagaatagcg ccccaaactc aggatagagt cttgattgtt gcggcatatt
     1321 gagtttccct acattttccc tacaaaaatt tggtttgcaa tcattagcat atgggcattg
     1381 tcatatacag acacatgtga ctgcgcaatt ttaacggacg ttcgaccaca ttgtgaatgt
     1441 gtatgtgatt cagtcactcg caaccggtag cgtacaggac tgaagcatga ggcaatttat
     1501 gtcacaaata cccgcacatc tcgatttttc gaaagatgag agacaaatgc atacaatcca
     1561 tgatcaagct acagagtggc gcggaatccc cgaaccactg agcgagatac tattgcaagt
     1621 ttttttgcgc cttcaggcta atacctcaga aaatggagat tcaaatgatt aacccatccc
     1681 ctaccaataa acacctacga ggagaaaata tgaacgagat taacaaagct gcggcgatgt
     1741 acattcgtta tctccctggt aattacaagg gactcaaaac ccaactcatc gagattacca
     1801 ggttggcaat ggctgaaggc atcacaatca ctcagttcta catcgatgag gtgtctcttg
     1861 tccagtctat tgagcgtcct gaacttcagc agttattaaa gttccaaaca ctttatgtct
     1921 acacgaaaga cagatttgca agggatgccc accaggcaaa cacgattttg acgcaattgg
     1981 catcaatgag tgtccatata cactttgttg ctgaaacact tcctccccat tctatggggt
     2041 aacccctgcc cttctcagcc caatgaccgc ctgttagcag tccccaacag gcggtttcaa
     2101 aaacactcaa gaccgctaat ccatgaataa taatcattta gaaacaggct ccgcctatat
     2161 ccgatactcc tccaccatgc aagatgactc tttcagtctc gatgcgcagc ttcgtcagat
     2221 caagatccgg gcagcttctg atggcgttga gattgtaaag gtctactctg acccggctac
     2281 ttctgcatac agcaaaaaaa atcgacctgg tctcgaagag atgcttgcgg atgccaaaag
     2341 agggctgttc aagtttgctt atgtacataa attggaccgc ctttcccgca ggcttgaatg
     2401 ggcactcgaa atcgtaaaga ttctccaaaa tgcaggcgtg acgttgcgtt ctgttgaaca
     2461 gaagtttgac ttggataccc ctgatggcaa actactgttt gcaatgttag gctcgctagg
     2521 tgagtttttc tcagacaacc tcagccagga gaccggtaag ggaaaacttg agcgagccac
     2581 tcagggatat cacaataatc aagtgccatg gggatacaag agtgaactgg tgggtgtcaa
     2641 gaagcaagct gttcctgacc cgaatactca agaaatcgta aaacaaatgt ttgaacgcta
     2701 ttcgactgga ctttattacg atcaagagat tgcagattgg ctgacagatc ttgggcacat
     2761 aacttcacgt ggcagacctt tcaccaaaga cacagtccgg gagatgctac taaacaagtt
     2821 ttatcttggc ttgaccaact acaacggcag gcatggtcat aagggaaaac gacatgaaaa
     2881 agaccgggtc tggatcaagg gtgtccattc ccctctgatc agtaaagaac tctttgaaaa
     2941 gtgtctgaaa gtccgtgcca gccgcaggcg tatgatgaat tccaaacaga agacgtttcg
     3001 agtttacttt ctggcaggca ttattacatg caa
//
LOCUS       DAOQJE010000410         3032 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_21744_length_3032_cov_4.696339, whole genome shotgun sequence.
ACCESSION   DAOQJE010000410 DAOQJE010000000
VERSION     DAOQJE010000410.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3032)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3032)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3032
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21744_length_3032_cov_4.696339"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..811)
                     /locus_tag="PLT08_10945"
     CDS             complement(<1..811)
                     /locus_tag="PLT08_10945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1565 domain-containing protein"
                     /protein_id="HNE05026.1"
                     /translation="MNTHGSISKFIVLGVTLSLLLSLLVPSGWVSARPEQALGTISFV
                     PNIETAGVVVSGTGLPATAQLSYRRAGDVNWQPGHALLRIKDGRLIGSLFNLAASTSY
                     EVRVSDGVTEVSGSFTTQADELLFAPTNIIYVNASAQASGDGSASAPFRTIQEGVNRA
                     APGTQVLVADGIYSESVTFPASGTAGSWIQVKAQGGGAVLDGSASVLATDWNPVEGKA
                     NVWFTKISPGIKYLARDGLRFYQYDFLSDLYDQRGHNNVPMSEGWFYDPING"
     gene            complement(925..1560)
                     /locus_tag="PLT08_10950"
     CDS             complement(925..1560)
                     /locus_tag="PLT08_10950"
                     /inference="COORDINATES: protein motif:HMM:NF014305.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE05027.1"
                     /translation="MTIPHETSPVYAAGAKLEEAKSAMILLHGRGASAQDILSLSTYL
                     NFPGMAYLAPQAEGSIWYPNRLIAPVESNEPYLSAAIAKIDGLVKHVEAGGIPVEKIF
                     IGGFSQGASLSSEFVIRNPRPYGGLLIFSGGYVWQSGKPRTAEGNLKGVPVFNGCSNV
                     DPHIPLERVKETTALLTSMGAEVNEQIYPNMDHTIVDEEIEQARALVEKRL"
     gene            complement(1557..2519)
                     /locus_tag="PLT08_10955"
     CDS             complement(1557..2519)
                     /locus_tag="PLT08_10955"
                     /inference="COORDINATES: protein motif:HMM:NF013098.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ring-cleaving dioxygenase"
                     /protein_id="HNE05028.1"
                     /translation="MNPIKGLHHVTAVARDPQRNVDFYRNVLGQRLVKRTVNFDSPDT
                     YHFYFADETGTPGSVLTFFAWPNMRRGVRGNGETNTVAYNVPVGSLSFWEKHLKQNGI
                     VPGGIQSRFNQNVLPFTDPDGMNLELVEVENLPAIQHWEDGPIPREYALHGFHSVTLW
                     VDEVEPTASLLTEGMGYRFAGAEEDRQRFKGDTNALGHIVDVVERPQKMPAGFGAGSI
                     HHIAFRVPTDDEQLKYQSVLGEAGFRVSPVMDRSYFHSIYFREQGGVLFEIATDTPGF
                     MVDEPKDLLGIKLKLPAWVEPNRDEIEASLLPLTLNPIEKVSLS"
     gene            complement(2619..>3032)
                     /locus_tag="PLT08_10960"
     CDS             complement(2619..>3032)
                     /locus_tag="PLT08_10960"
                     /inference="COORDINATES: protein motif:HMM:NF013713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE05029.1"
                     /translation="FGYLEENPGTFDVIISDTTDPIGMAERLFSDEFYKLMVRALAPG
                     GAAATQCEQPFFDTELIKTIYQTAKSLTKNPAYYYTNVPTYPGGGIGFMYVSNTPWEN
                     GLKTPYPPGVNQYINPEIHKAAFALPEFFRKELYG"
BASE COUNT          789 a          785 c          716 g          742 t
ORIGIN      
        1 gaccattgat agggtcataa aaccaacctt cactcatcgg cacattgtta tgtccgcgtt
       61 ggtcgtaaag gtcactcaaa aaatcatatt gataaaaacg caatccatcc cgcgccagat
      121 acttaattcc cgggctaatc ttcgtaaacc agacattggc tttgccttca acaggattcc
      181 aatccgttgc taaaaccgaa gcggacccgt ctaagacagc gcctcctccc tgcgccttga
      241 cctgaatcca gcttccagcc gttcccgaag ccgggaatgt gaccgactca ctgtaaatcc
      301 catctgccac caaaacttgc gtgcctggtg ctgcacgatt gaccccttcc tgtatcgtac
      361 gaaatggcgc agaggcagac ccatccccac ttgcctgtgc agatgcattt acataaataa
      421 tattcgtggg cgcaaaaagt aactcatcgg cttgagtggt aaacgaacca gaaacttctg
      481 ttacaccatc agaaacgcgc acttcataag aggtagatgc agccaagtta aacaagctac
      541 caatcaaacg tccgtctttg atgcgcaaaa gggcatgccc tggctgccag tttacatccc
      601 ctgcccggcg gtaagacaac tgagccgtgg ccggcagccc cgttccgctg acgacaactc
      661 ccgccgtttc aatattgggc acaaaactaa ttgtccccaa agcctgttct gggcgtgcac
      721 tgacccatcc actcggcaca agcagtgata acaggagcga aagggttaca cccagcacta
      781 taaatttcga tatactacca tgcgtgttca taaaaaccat ttacagggat ggaaacaaaa
      841 ccctgtctgt tttcactata gcacgcacct ataaaaatac aattctggta ccatgacaaa
      901 aaacgcccct ttcggggcga tttcttataa ccttttctca accaaggctc ttgcttgctc
      961 gatctcttcg tccacaatcg tgtggtccat gtttgggtaa atttgctcgt ttacttctgc
     1021 ccccatggaa gtcaggagtg ccgtggtctc tttcactcgc tccaacggaa tatgaggatc
     1081 cacattcgaa cagccgttga aaaccggtac acctttcaag ttgccttccg cagtgcgggg
     1141 tttcccgctt tgccaaacat atccgccgct aaagatcaac agcccaccat acgggcgcgg
     1201 attgcgaatc acaaattcgc tggaaagact ggcgccttga gagaaaccgc caataaatat
     1261 tttttcgaca ggaataccgc ccgcttcaac atgttttacc aacccatcga tcttcgcgat
     1321 ggcggctgaa aggtacggtt cattggattc aacaggcgcg attaaacggt tcgggtacca
     1381 aatggatcct tccgcttggg gggcgagata cgccatgccc ggaaagttca agtatgtgga
     1441 aagggacaga atatcttgtg ccgatgcgcc acgtccatga aggaggatca tcgcagattt
     1501 ggcttcttct agctttgcac ccgctgcata gacaggggat gtttcgtgag gaatggtcat
     1561 gaaaggctca ctttctcaat agggttaagg gttaatggca gtaagctggc ttcgatctca
     1621 tctcgattgg gttctaccca ggcgggcagt ttcaacttta tgcccagcaa atccttgggt
     1681 tcatcgacca tgaaaccggg cgtatcggtt gcgatctcaa acaacacacc gccttgctcc
     1741 cgaaaataaa tggaatggaa atagctgcga tccattaccg ggctgacgcg aaaccccgcc
     1801 tcacccaaga cggactgata tttcagttgt tcatcatccg tgggcacgcg aaaagcaata
     1861 tgatggatgg aaccagcccc aaaccctgca ggcatctttt gcgggcgctc aaccacatcc
     1921 acaatgtgac cgagggcatt cgtatcaccc ttaaagcgtt ggcggtcttc ctctgccccg
     1981 gcgaagcgat agcccatgcc ttcggtcaag agagaagcgg ttggttcaac ttcatccacc
     2041 cacaaagtga cgctatgaaa tccatgcaaa gcatattcac gtgggatggg tccatcttcc
     2101 caatgctgaa tggcgggcaa attttccact tccaccaatt ccaggttcat gccatcgggg
     2161 tccgtaaaag gaagaacatt ctgattaaag cggctttgaa taccgccggg gacaataccg
     2221 ttttgtttca ggtgtttttc ccaaaaggaa agtgaaccaa ccgggacatt atatgcaacg
     2281 gtattggttt cgccattgcc gcgcacgccg cgccgcatat tgggccaagc gaagaaggtc
     2341 aatacactgc cgggtgtgcc ggtttcatct gcaaaataga agtgataggt atcgggcgag
     2401 tcgaagttaa ctgtgcgctt gaccaagcgt tgccctaaaa catttcgata aaaatccaca
     2461 tttcgttgtg ggtcacgtgc cacggctgtg acatgatgta aacctttaat agggttcata
     2521 tttctctcct attgcctaga cgcactctat cgcaaaaata tccgtttgaa tttcgtatga
     2581 aaagcctcca aagttaattt tggaggcttg gtcttatttt acccgtacaa ttctttgcgg
     2641 aagaattctg gcaaagcaaa cgccgctttg tggatctctg ggttgatgta ttggttcaca
     2701 ccgggtggat agggcgtttt caaaccgttt tcccaaggag tgttcgaaac gtacatgaac
     2761 cctatcccac cgccggggta tgtggggacg ttggtgtagt aataggcggg attcttggtg
     2821 agtgatttgg cggtttgata aatggttttg atcaactcgg tgtcaaagaa aggctgctcg
     2881 cattgagtcg cagcggcacc accgggagca agggcacgta ccatcaactt gtaaaattca
     2941 tcggaaaaga gacgctctgc catgccaata ggatcggtgg tatctgagat gatgacatcg
     3001 aaggtgccag ggttttcttc taggtagcca aa
//
LOCUS       DAOQJE010000411         3031 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_21755_length_3031_cov_5.211022, whole genome shotgun sequence.
ACCESSION   DAOQJE010000411 DAOQJE010000000
VERSION     DAOQJE010000411.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3031)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3031)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3031
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21755_length_3031_cov_5.211022"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..433
                     /locus_tag="PLT08_10965"
     CDS             <1..433
                     /locus_tag="PLT08_10965"
                     /inference="COORDINATES: protein motif:HMM:NF013645.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE05030.1"
                     /translation="IGGEITKPLNDSLGFIGARANVKGFLCEYMTAGRVLVLGDPGPW
                     MCAGMTGGVLYLRLQPHLHFDYPAIQRRLAKGAKVSVQAVDDADFQNLQHLLTIYAEE
                     LRQNHQADEADNVLGLLIDWKNTFIRVVPEGLQEDQRFATE"
     gene            664..1968
                     /locus_tag="PLT08_10970"
     CDS             664..1968
                     /locus_tag="PLT08_10970"
                     /inference="COORDINATES: protein motif:HMM:NF014135.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HNE05031.1"
                     /translation="MIFTIIIFLLAYIAIASEKFPRHWVALLGGSLLILFGVLTPSEA
                     LSYISWETLGLVAGMFTLVSILVNAGFFTWLAMTSLRKVNYHPVPLFIILILLAAFLA
                     AFMDSITVMLFLSALTLQLCTLLKIDPVPLVIAEVCAANTGGAATLVGDPPNVILGTT
                     LGFSFADFATNTGPIAIVGALILMAYAYIKNRKSLKEAHIALTPEMIDQIDELHNEPL
                     HVHLTRVGLTGFMMAVILLIFHIPLSTLTGLPINAAVSALIPAGIALIALRDDQRKKV
                     IISVDGESILFFAGLFMLIGGLEKVHLFETLAESLALATASNPNSLIMALHWGPGLAS
                     GILDNVPLALAMTFVLKDLAKIPGTPALSMMVWSLAMGVDVGGNLTPIGASANVVAYS
                     YIEHNHGNIGWKRWLAIAVPPTLIVMIVTSIILLYKYQIGWY"
     gene            1992..2462
                     /locus_tag="PLT08_10975"
     CDS             1992..2462
                     /locus_tag="PLT08_10975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE05032.1"
                     /translation="MYALSIVGYIMIALLLMNMMAKARPQIKEPMPGKALKSLPMGLA
                     SRFRLQRSSIMALLTGIVFGVVTGWLSLSTAGIVIAVALATIFLPMQYTFTTKGIALG
                     QGMFYPWSDFSGFTSKGSTLQFERSSVFGGLTVSIASTEVDTVLKYAERYIETR"
     gene            2486..>3031
                     /locus_tag="PLT08_10980"
     CDS             2486..>3031
                     /locus_tag="PLT08_10980"
                     /inference="COORDINATES: protein motif:HMM:NF013104.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE05033.1"
                     /translation="MKALNKRKLKFVLPLLVMLLAFVLVTPAFAQDGNPPTTEQVDDI
                     ALGVNMLWMLLGGFLVFFMQAGFALVETGFTRNKNVVHTMMMNMMVFCIGAVGYWLTG
                     FAFQFGGVNAAYPEVSTVGAIAGEWAHSPITLGDWSGLLATPLLRFGQFGLLGGSGFA
                     LAGVEANAGILAFFLFQMVFMD"
BASE COUNT          711 a          739 c          709 g          872 t
ORIGIN      
        1 catcggcggc gagatcacca aaccgctcaa cgacagcctc ggcttcatcg gggcgcgtgc
       61 caacgtcaaa ggtttcttgt gtgaatacat gaccgccgga cgcgtgctcg tgctcggtga
      121 ccccggtccg tggatgtgcg caggcatgac cggcggtgtc ttgtatctac gcttgcaacc
      181 tcatctacac tttgactatc ccgcaattca acgccgcctc gccaagggcg cgaaggtctc
      241 tgtgcaagct gtagatgatg ccgacttcca gaacttgcag cacctgctca ccatctatgc
      301 tgaagagtta cgccaaaatc atcaagccga cgaagctgat aatgtgctgg gtctgttgat
      361 cgattggaag aataccttta tccgtgtcgt gccggaaggt ttacaggaag atcagagatt
      421 cgctaccgaa taacaacgta cccaaacggg tgaaattaaa tcaactgcgc catgagaaat
      481 tgtttcatgg cgcggttgtc atttaaacgt gctccatttg gggcatcttt tcaatgtgat
      541 ctatagttgt ttgagcacaa catgaaatga atacccatta gggtaatagc catacaggtt
      601 caattccata caataaggcg cattacataa aacgttattc atcttttgag gagagatgcc
      661 cctttgatct tcaccatcat catctttttg ttggcatata tcgcgatcgc aagtgagaag
      721 tttccacgcc attgggttgc ccttttgggt ggttcacttc taatcttgtt tggtgtcctc
      781 actccatcag aagctctttc atacatcagc tgggaaactc taggtttagt tgctgggatg
      841 tttaccctcg tatccatttt ggttaacgct ggctttttta cttggttagc catgacatct
      901 cttcgcaaag tcaattacca ccctgtgccc ttattcatta ttttgatcct tttggctgca
      961 ttcctggcag ccttcatgga cagcattaca gtaatgctgt tcctttctgc attgacatta
     1021 caactctgca ctttgttgaa gattgacccc gtaccacttg tgatcgcaga agtctgtgct
     1081 gccaacactg gcggcgcagc cacattggtc ggcgaccctc ctaatgttat tctaggaact
     1141 acattggggt tcagttttgc tgatttcgcc acaaataccg gtccaatcgc cattgttggt
     1201 gcactgatcc tcatggcata tgcctatatc aaaaatcgca aatcgttgaa agaagctcac
     1261 atcgcgctca caccagagat gatcgaccag atcgacgaat tgcataatga accgcttcat
     1321 gttcacctga ctcgtgtcgg gctaaccggt tttatgatgg cagtcatcct cttgatcttc
     1381 cacattcccc taagcacgct aaccgggcta cccatcaatg cggcagtatc ggctttgatc
     1441 cctgctggca tcgcactgat cgccttaaga gatgaccagc gcaagaaagt cattatcagc
     1501 gtagacggtg aaagcattct attctttgcc ggtctcttta tgctcattgg cgggctggag
     1561 aaagtccatc tcttcgaaac tcttgctgag tcattggctc tggcaactgc atccaaccca
     1621 aattccttaa tcatggcatt gcactgggga ccagggcttg ccagtggcat tctagacaat
     1681 gtccctctag ctttggcaat gacctttgtg ctcaaagacc tagcaaaaat ccccggtaca
     1741 cctgcacttt ccatgatggt ttggtcattg gcgatgggtg ttgatgtagg cggtaattta
     1801 actccaatcg gagcatccgc caatgtcgtt gcttactcct acatcgaaca caatcacgga
     1861 aatatcggtt ggaagcgctg gttggcgatt gctgtgccgc ctacgttaat cgtcatgatc
     1921 gtcacatcga tcatcttgct ctataaatat caaatcggtt ggtactaatt ttttcaagga
     1981 gagaagaaat catgtacgca ctttcaattg ttggttacat catgatcgcc ttgttgttga
     2041 tgaacatgat ggcaaaagcc cgtccacaga tcaaagaacc catgccagga aaagcattga
     2101 aaagcctacc catggggttg gcgtcccgtt ttcgtttgca gcgctcaagc atcatggcgt
     2161 tgttgacagg gatagttttc ggggtcgtta cgggttggtt atcgctcagc actgcaggga
     2221 tcgtcatcgc agttgccctt gccaccatct ttttaccgat gcaatacacc ttcacaacaa
     2281 agggaattgc gcttgggcaa ggaatgttct acccgtggag tgatttttca gggtttacga
     2341 gcaaggggtc aactctacaa tttgaacgct cttcagtttt tggaggtctt acagtatcca
     2401 tcgcctccac cgaggtggat actgttttga agtatgccga acgctatatt gaaacaagat
     2461 aaaaactcaa aagaggagaa tgaaaatgaa agcattgaat aaaagaaagc taaagtttgt
     2521 tttgcctttg ttggtcatgt tattagcttt tgtgcttgta acacctgctt ttgcacagga
     2581 cggaaaccca cccaccactg aacaagtgga tgatattgcc ctgggtgtca acatgctttg
     2641 gatgttgctt ggcggtttct tagtcttctt catgcaggct gggttcgctc tggtggaaac
     2701 cggcttcacc cgtaacaaga acgtcgtcca caccatgatg atgaacatga tggtcttctg
     2761 tattggtgct gttggctact ggttaaccgg ctttgccttc cagttcggcg gtgtgaatgc
     2821 tgcctaccca gaagtatcca ctgttggcgc tatcgctggt gaatgggcac actccccgat
     2881 cacccttggc gattggagcg ggcttttagc aacccccttg ttacgctttg gtcagtttgg
     2941 tcttctcggt ggaagtggtt ttgcattggc tggtgtagaa gcgaacgctg gtattctcgc
     3001 cttcttctta ttccagatgg tcttcatgga t
//
LOCUS       DAOQJE010000412         3031 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_21758_length_3031_cov_4.140793, whole genome shotgun sequence.
ACCESSION   DAOQJE010000412 DAOQJE010000000
VERSION     DAOQJE010000412.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3031)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3031)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3031
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21758_length_3031_cov_4.140793"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..219)
                     /locus_tag="PLT08_10985"
     CDS             complement(<1..219)
                     /locus_tag="PLT08_10985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE05034.1"
                     /translation="MNPPECEIYILNYVNHRARSWRETDRLIQTRSLLATLMAFLRTV
                     NVTRLADLTPRVWFAYVQERIKAGIKPSS"
     gene            complement(216..1073)
                     /locus_tag="PLT08_10990"
     CDS             complement(216..1073)
                     /locus_tag="PLT08_10990"
                     /inference="COORDINATES: protein motif:HMM:NF012798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HNE05035.1"
                     /translation="MDFNPTPFSDWLICQYPRSSARKHYLSDLALFFSWIGKPPSEIS
                     PQDVAQYIHHCLEKSLSPLTINRRLSSLRLFYYFLRIIQEEVLDCPVQKRHYLRKPQP
                     LPRDASEEQITALFSIITDKRDKAIFTLMLECGLRVGEVCALSLDDVLLEDPPRLKVH
                     GKGGRQRIAYLPPPALSVLTDWLTSRPVTKDRAVFISQRVKRLSVNGIQFILKEYFQK
                     AGIQVTCHQFRHAFGRRMVEASMPITSLQKLLGHRSLRTTQRYVNLSNPALQADYDHA
                     IQKALGGVS"
     gene            complement(1155..2171)
                     /locus_tag="PLT08_10995"
     CDS             complement(1155..2171)
                     /locus_tag="PLT08_10995"
                     /inference="COORDINATES: protein motif:HMM:NF024753.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HNE05036.1"
                     /translation="MISLQRLQRWLSRRGTLVLFLLLAIVVWALPGWFEKKTPTITHT
                     LALSTIPAETIWLNQEVFTKTIYNDNGVMYAMPVSRENIAAIDINNGNVIWKADLPLE
                     RGGGARGLLALSDKVFVITSMFIDAYEASTGNLKWSTQLGKGHVRVLAQIESDLVRIY
                     YGDNIIEVGQETGAIIRTYPANNVKWKIGNLSLAASPSNYLIAFNNQNDEPLWKNDLP
                     FFIGEELEPIDVGNGELIVGFVDGICKLKLMNGTYVWCHPEINIADTAVDIPSQRVFA
                     MTNDLEIFIIDLQTGDMIGQSRFLSSAPMTNERSVIASLEFGDGVLVVSFNDSGQTFG
                     IKLK"
     gene            complement(2226..>3031)
                     /locus_tag="PLT08_11000"
     CDS             complement(2226..>3031)
                     /locus_tag="PLT08_11000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE05037.1"
                     /translation="SLNRWMYVEGNPVNAVDPSGFTPLRLGYMEGYSTSAGLLDGHIQ
                     GNEIVYDYATMSRARFRYSGIGGLAFVSVGAAIYMGEITGFNYEPKPLPIGLDGSDKL
                     PYSNLIVEDYSGGSTGVYGGISIGLTIGMGYFQSDTGSVKGVFSYASLSAGLPVEVVG
                     FHTTYDVASGIEYYYDPDTGHVNRAKLISDILSGNKSPIPFGVLPSLTSYVGGARTGQ
                     ISPILLAARRFEKYYYRPQYGQCRDGIPDPPSDLELPYPFDIPSPFPVP"
BASE COUNT          821 a          685 c          699 g          826 t
ORIGIN      
        1 cgaggaaggt ttgatgcctg cttttatcct ctcctgaacg taggcaaacc agaccctggg
       61 cgtgaggtct gctaggcggg tgacattcac tgttcggaga aatgccatga gggtggcaag
      121 taagctgcgg gtctgaatga ggcgatctgt ctccctccag gatcgtgccc gatgattcac
      181 atagttgaga atataaatct cacattctgg tgggttcatg acacacctcc caatgccttt
      241 tggatcgcgt ggtcatagtc agcttgcagc gcagggtttg agagattgac atagcgctgt
      301 gtggtacgca gactgcgatg tccgagaagt ttttgcaggg atgtgattgg catggatgcc
      361 tctaccatac gccgcccgaa ggcatgacgg aattgatgac aggtgacttg aatgcccgct
      421 ttttggaaat attctttcag gatgaactgg ataccattga cggagagccg cttgacacgc
      481 tgggaaataa aaaccgcccg atctttcgtg accgggcggc tagtgagcca atctgtgagt
      541 acggataagg ctggtggagg gagataggca atgcgttgcc tgcctccctt gccatgaact
      601 tttagccggg gagggtcttc gagaagaaca tcgtccaagg agagagcgca gacttcaccc
      661 acccgcaaac cgcattcgag catgagggtg aagatggctt tatcgcgttt gtcggtgatg
      721 attgagaaca gagcggtgat ttgttcctcg ctggcgtcgc gcggaagggg ttgaggctta
      781 cgaagataat gacgtttctg gacaggacaa tccaaaacct cttcctgaat gatcctgagg
      841 aaataataaa acaaccgcag tgaggaaagc ctgcggttga tcgtgagagg agataacgat
      901 ttttcgagac agtggtggat gtattgggct acatcctgtg gggatatttc cgagggtggt
      961 tttcctatcc atgaaaagaa aagcgccagg tcactgagat agtgtttcct ggcgcttgaa
     1021 cgcggatact ggcagatcag ccaatcactg aatggggtgg ggttgaagtc catgataatc
     1081 tcctattgtt gggtttatgg gagattatca taattgactc ttaatctaaa ttttgcaaca
     1141 ttattttgta ggcactattt aagttttatc ccgaatgttt gcccgctgtc gttaaatgac
     1201 acaactaata caccatcgcc gaactccagt gaggctataa cgcttctttc atttgtcatg
     1261 ggtgcgcttg atagaaaacg cgattgaccg atcatatccc cggtttgaag atcaatgata
     1321 aaaatctcaa gatcgttagt catggcaaaa acacgctgag agggaatatc cacggccgtg
     1381 tccgcgatat tgatctctgg gtgacaccaa acatatgtcc cattcattaa tttgagttta
     1441 caaatcccat caacaaatcc aacgatcaat tctccatttc cgacatctat aggttccaat
     1501 tcttcgccta taaagaaagg aaggtcgttt ttccaaagcg gttcatcatt ttgattgttg
     1561 aaagcaatca agtaattgga aggcgaggcc gccaaggaaa ggtttccgat tttccatttg
     1621 acgttatttg ccggataggt tcttatgata gctcctgttt cctgtccaac ctcgattatg
     1681 ttgtcgccat agtaaatcct gaccagatct gactctattt gggcaaggac acgcacatgc
     1741 cctttaccca actgcgtaga ccatttcaag tttccggttg acgcttcgta tgcatcaatg
     1801 aacatgcttg ttatcacaaa aactttatca ctgagagcta agagtcctct tgccccgccg
     1861 cctctttcaa gaggcagatc agctttccat ataacgtttc cattgttaat atcaatggct
     1921 gcaatgtttt cgcgtgaaac tggcatcgca tacatcaccc cattgtcgtt ataaattgtt
     1981 tttgtgaaaa cctcttgatt aagccagatt gtttccgctg gaattgttga taacgccaga
     2041 gtgtgtgtaa tggtcggcgt ttttttctca aaccatcccg gcaatgccca tacaacaatg
     2101 gctaacagca aaaagagcac caacgtacct ctgcgggata gccatctttg aagtctttgt
     2161 aaacttatca taaacatctc ttatatagta tcactcattt ttcaatattc tcatcaatcc
     2221 gattgtcagg gaacagggaa aggtgatggg atgtcaaaag gatacggcag ctccaaatcg
     2281 gatggtggat ctggaatgcc atcacggcat tgaccatatt gtggccgata gtaatatttc
     2341 tcgaaacgac gtgcagctaa caaaattgga cttatttgac cagttctcgc tccacctaca
     2401 taagaggtca gacttggaag tacgccaaaa ggaataggag atttatttcc tgaaaggatg
     2461 tcggatatta attttgctcg attgacgtgc ccagtatccg gatcgtagta gtactcaatc
     2521 ccagaggcaa catcgtatgt tgtatgaaag cccacgacct ctactggaag tcctgcacta
     2581 agactggcgt acgaaaatac accctttaca cttcctgtgt cagattgaaa atagcccatc
     2641 ccaattgtca agcctataga gattccaccg tacacgccag tagatccacc gctgtaatct
     2701 tcaacgatta agttactata aggtaattta tcacttccat ccaaacctat tggcaatggt
     2761 tttggttcat aattaaaacc agtaatttct cccatataaa tcgctgcgcc aactgataca
     2821 aaggcaagtc cgcctatgcc tgaatatcta aaacgagccc tcgacattgt tgcatagtca
     2881 tatacaattt cattaccttg aatatgtcca tcaagaaggc cagctgaagt agagtatcct
     2941 tccatataac ccagtcgcag tggtgtaaag cccgaagggt caactgcgtt caccggattc
     3001 ccctccacat acatccaccg gttcagcgaa a
//
LOCUS       DAOQJE010000413         3029 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_21778_length_3029_cov_8.461668, whole genome shotgun sequence.
ACCESSION   DAOQJE010000413 DAOQJE010000000
VERSION     DAOQJE010000413.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3029)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3029)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3029
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21778_length_3029_cov_8.461668"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1350
                     /locus_tag="PLT08_11005"
     CDS             <1..1350
                     /locus_tag="PLT08_11005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE05038.1"
                     /translation="WVDFRGSYEAGIKELISQLNQPIQETQAPPESGFKAPFIVWVSI
                     LLSAIAGVYSLLSFWSVLLPLILVPLAWQVYKRNYNFSRVQTALWMSPLLNLLGLLLF
                     SELGYLNAEGENFAVYYFRINPLLGVLVNILQWLPYSAFVLIFLFRSASMQRWGKPEA
                     NMPKFANPYRPKNPNPRPVKFFIDHAEQDSVIAKDISNKLIQYGHLQEADIKSAESVL
                     VLISRFNTDTTADPEHQIVFPVLVQTAQPSEKLSHVQWIDYRKGLKNLDALAQLLPDP
                     TLLLTALGTRPTNGTQIVMPNIFSRVVNFLTFILLLNVSTFFIYILELWANGLGYIQQ
                     YEPAFIFSNLAQTALAVLVACGLGFFIIKSVTERRGLFASIPPFFAGLFGLFLILMYQ
                     YTLADYMTTLFEDYGETEIYGFITIPYLFLIFGGIVLLIMIFFNWKELRRWFPKKGK"
     gene            1369..>3029
                     /locus_tag="PLT08_11010"
     CDS             1369..>3029
                     /locus_tag="PLT08_11010"
                     /inference="COORDINATES: protein motif:HMM:NF025056.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003953 - NAD+ nucleosidase activity
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="toll/interleukin-1 receptor domain-containing
                     protein"
                     /protein_id="HNE05039.1"
                     /translation="MMPNTFISYSRQCIGFVDDLAHKLQKQGFSIWTDYLSLVPGRPW
                     AEQIEKGLQEAELLLLVVSPESVSSRSVEIEWRHFLQHKKRIILLIFQAVPLPPELAG
                     LEWVDFRGAFAPAMKELLKRIEAPSPQRGLAPQSGFKAPFVVWVAAGLSVINAVYSLF
                     AFWTLLIPWVLVPLPARIFKREFNLSQVQTALWTQPIALFFTLGLFLELKLINIDVDF
                     DYSNYFSLYNMVLMSLFIQIYLIPFFSILLLIVLRLPGMQRWGRPEASAPKFANIYRP
                     NIVKPDPVHYFIDYAPEDKAIARELEKELTKVGHTPANGLASANEALVLLSRFKTDTE
                     ADPEKQVVYPILIQRVKLPEKLSKVQWIDFRKGVRNLEAIAKLLPQPAKLLTALGVRP
                     TSSGQAAMPNIVIALVDFLIIMGMVNLGSFFLYMFELTSLNLRLVAEYESFRLLWMIF
                     MQLLSTVASGWLIYFMVRSLSDRIGWFTKPLALSLGFIVILLLFFWQLILGSNINDLL
                     LHYGIVAETLFYALPFMFMFTGLCIVIVLVLFRFKALRMWLPAKG"
BASE COUNT          699 a          786 c          665 g          879 t
ORIGIN      
        1 tgggtggatt ttcgaggtag ttatgaagct ggcatcaaag aattaatttc acaactcaat
       61 caacctattc aagaaacaca agccccgcct gaaagcggat tcaaagcgcc cttcatcgtg
      121 tgggtaagca tccttttgag cgccatcgct ggcgtttatt cgctgctttc cttttggagc
      181 gtattgcttc cattgattct ggttccgctc gcctggcaag tctataaacg caactataac
      241 ttctcacgcg tccaaactgc cttgtggatg tcacctttat taaacctgct tggcttacta
      301 ctctttagtg aacttggtta ccttaatgcg gaaggcgaaa attttgcagt atattatttt
      361 cgcatcaacc cattattggg ggtattggta aacatcttac agtggctgcc ctattcggct
      421 ttcgttttga tctttctttt tcgttcggca agtatgcaac gttggggcaa gcccgaggcg
      481 aacatgccga aatttgccaa tccatatcgt ccgaaaaatc ccaatccccg ccctgttaaa
      541 ttctttatcg atcatgcaga acaggattct gttatcgcga aggatatatc aaacaagttg
      601 atccagtatg ggcatcttca agaagcggac atcaaatctg ctgagtcagt gttggtatta
      661 atctcgcgct tcaacaccga cacgaccgct gaccccgaac atcaaatcgt tttccccgtt
      721 ttggttcaga ccgcacaacc ttcagaaaaa ctttcgcatg tgcaatggat tgattatcgc
      781 aaagggttga aaaatttaga cgcattggca caattattac cagacccaac cctattatta
      841 actgcattgg gcacgcgtcc caccaatggc acacaaattg tgatgccaaa catcttcagt
      901 agagttgtca acttcttaac ttttattctt ttgctaaatg tcagtacgtt ttttatctat
      961 attctggagc tatgggcaaa tgggttggga tacatccaac agtatgaacc ggctttcatc
     1021 tttagcaacc tggcgcaaac ggcattggct gtgcttgtgg cgtgcggact tggatttttc
     1081 ataatcaaat cggtaacaga aaggcgcgga ctctttgcat ccatcccgcc gttttttgca
     1141 gggctatttg gtctgtttct cattctcatg tatcaataca ccctggcaga ttacatgacc
     1201 acactcttcg aagactatgg cgaaacagaa atttatggtt ttattaccat tccatatttg
     1261 tttcttattt ttggcggaat tgttttgctg attatgatct tcttcaattg gaaagagtta
     1321 cgccgttggt tccctaaaaa agggaaataa atttttaacc tctaccctat gatgcccaac
     1381 acctttatca gttattcacg gcaatgcatt ggttttgtag atgaccttgc ccacaagttg
     1441 caaaaacaag gcttcagcat ctggacagat tacctcagcc tggtgccggg gcgaccatgg
     1501 gcagaacaga tcgaaaaggg acttcaagaa gccgagctgc tgctcttggt cgtctcgcca
     1561 gagtctgttt cctctcgcag cgtggagatc gaatggcggc actttctcca gcataaaaag
     1621 cgcatcatct tattgatctt tcaagccgtg cctttgccgc cagagctagc cggcttggaa
     1681 tgggtggact ttcgcggtgc ctttgcccca gccatgaaag aactcctgaa acggatcgag
     1741 gcgcccagcc cacagcgtgg actcgctccg cagtctgggt tcaaagcccc gttcgtcgtt
     1801 tgggtggcgg cgggtctcag cgtcatcaat gccgtttatt ccctcttcgc tttttggact
     1861 ctgttgattc cctgggtgtt agttcctctg cccgcgcgca tcttcaaacg cgagttcaat
     1921 ttgtcgcaag tccaaaccgc gttgtggaca caacccattg ctttgttttt cacactgggc
     1981 ttgtttctcg aattgaagct catcaacatc gatgtagatt ttgactacag caattacttc
     2041 tcgctctata acatggtgtt gatgagtctc ttcatacaga tctatctcat ccccttcttt
     2101 tccatcttgc tgttgatcgt cttacgctta cccggcatgc aacgctgggg cagacccgaa
     2161 gccagcgccc caaagtttgc caatatctat cgcccgaata ttgttaagcc agaccccgtc
     2221 cactacttca ttgattacgc cccagaagac aaagccattg cccgcgagct ggaaaaggag
     2281 ttgaccaagg ttggtcacac ccccgcgaac ggactcgcat ccgcgaatga ggcgctggtg
     2341 ttgctctcgc gcttcaaaac ggacaccgaa gcggaccctg aaaaacaggt ggtgtatccc
     2401 atcctgatcc aacgcgtgaa actacccgag aaactctcca aggtgcagtg gatcgacttc
     2461 cgcaaagggg tgcgcaatct cgaagccatc gccaagcttc tgccgcagcc tgccaaacta
     2521 cttacagctt tgggcgtccg ccccaccagc agcggacaag ccgccatgcc caacatcgtc
     2581 atcgcccttg tagactttct catcatcatg ggcatggtaa accttggctc tttcttcctt
     2641 tatatgttcg agctgaccag cctcaacctg cgcttggtgg cagagtatga atcctttcgc
     2701 ctgctgtgga tgatctttat gcaattgctc tccaccgtcg cttcgggctg gttgatctat
     2761 ttcatggtcc gttcgttgag tgaccgcatc gggtggttta ctaaaccctt ggcattgtcg
     2821 ctaggcttca tcgtcattct attgctgttc ttttggcaac tcattcttgg ttcaaatatc
     2881 aatgaccttt tacttcacta tggcattgtg gcagaaactc tcttttatgc tctgcctttc
     2941 atgttcatgt tcacagggct gtgcattgtg atcgtgctcg tgctcttccg cttcaaagcc
     3001 ttgcggatgt ggctgccggc aaaaggaaa
//
LOCUS       DAOQJE010000414         3028 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_21793_length_3028_cov_5.193071, whole genome shotgun sequence.
ACCESSION   DAOQJE010000414 DAOQJE010000000
VERSION     DAOQJE010000414.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3028)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3028)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3028
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21793_length_3028_cov_5.193071"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..404
                     /locus_tag="PLT08_11015"
     CDS             <1..404
                     /locus_tag="PLT08_11015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="PLP-dependent transferase"
                     /protein_id="HNE05040.1"
                     /translation="RMDRHVQNAQAVAEFLEKHPKVSWVKYPGLKSHPDYDRAKKYLP
                     KGAGAILGFGIKGGAAAGKKFIESLQLFSHLANVGDARSLAIHPATTTHSQLTEEQQV
                     TAGVSPDFVRLSIGLEDFEDIKWDLDQALNA"
     gene            478..1404
                     /gene="metA"
                     /locus_tag="PLT08_11020"
     CDS             478..1404
                     /gene="metA"
                     /locus_tag="PLT08_11020"
                     /EC_number="2.3.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008899 - homoserine O-succinyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine O-succinyltransferase"
                     /protein_id="HNE05041.1"
                     /translation="MPVKIPSTLPAREILEYENIFVIDEERALHQDIRALRVAILNLM
                     PTKVATETQILRLLSNSPLQVEITLVHTATHESKNTDAEHLLQHYVTFEDIKHEKFDG
                     LIITGAPVEQLPFEEVDYWEELKQVMDWAKTNTESTFYICWGAQAGLYHYYGVPKYDL
                     PQKMFGVFEHRLLNRTESLMRGFDDIFLAPHSRHTEIRREDIEKVPELQILAESDDAG
                     VYIVGSRDGRELFITGHSEYDPLTLKAEYDRDVNKGLPIHVPKNYYPNDDPKQMPNVR
                     WRGHANLLYANWLNYYVYQATPFDPNEIPKRN"
     gene            1404..2690
                     /locus_tag="PLT08_11025"
     CDS             1404..2690
                     /locus_tag="PLT08_11025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetylhomoserine
                     aminocarboxypropyltransferase/cysteine synthase"
                     /protein_id="HNE05042.1"
                     /translation="MTSKHNFGFTTRQIHEGYTPDPATGSRAVPIYQTTAYDLQSTDR
                     AARLFALQENGNIYTRISNPTSNVFEKRVASLEGGIGALEVGSGHAAQATAILTICEA
                     GDHIVSTSLLYGGTVTQFKYTFPKLGITVTLVDPSDPENFRRAIQPNTKIIYGETISN
                     PLGNIFPFEEISKIAHEHGIPLMIDNTFATPYLCRPFEWGADIVTHSTTKFIGGHGTT
                     MGGIIVDSGKFNWSKSGRFKNFTEPDPAYHHVIYADDFGPLAFISKARAGILRDLGTV
                     QQPIASWFLIQGLETLSLRMTRHVQNAKRAAEFLENHPNVKWVSYAGLPSHPDHERAK
                     KYLPQGTGAVLGFGVKGGREAGAKFIDNLQLFSHVANIGDTRSLAIHPASTTHSQLSD
                     EEQTAAGVSPDFIRLSIGLEDIEDILWDLDQALSLI"
     gene            2739..>3028
                     /locus_tag="PLT08_11030"
     CDS             2739..>3028
                     /locus_tag="PLT08_11030"
                     /inference="COORDINATES: protein motif:HMM:NF024698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNE05043.1"
                     /translation="MPKITLRSVLPSDLPILFQQQLDPEAVAMSAYPAKDRGEFMRHW
                     EGIMKNKNVIARTIVYKEKVAGHIICWKEGKYEQRIGYWIGKEFWRRGIASTA"
BASE COUNT          844 a          849 c          654 g          681 t
ORIGIN      
        1 tgcgcatgga tcgtcacgtg cagaacgcgc aagccgttgc tgaattcctt gagaaacatc
       61 ctaaagtcag ctgggtcaaa tatcctgggc tcaagagcca cccagactac gaccgcgcca
      121 agaagtattt gcccaaaggc gcaggtgcga ttctcgggtt cggtatcaag ggcggtgcag
      181 ccgcgggcaa gaagttcatt gaaagcctgc agctcttcag ccaccttgcc aacgttggtg
      241 atgcccgctc tctcgccatt catcccgcta ccaccactca cagtcagttg accgaagaac
      301 aacaggtcac cgctggcgtc agtcctgatt tcgtccgctt gagcattggt ctcgaagatt
      361 tcgaagacat caagtgggat ctcgatcagg cgcttaacgc ctaaacatag aaattacatg
      421 tggaatgaag aaaaaaaaat tcttcattcc acatgcataa atcatcattt caaactcatg
      481 cctgtaaaaa tcccttctac tcttcctgct cgtgaaattc tcgaatacga gaatatcttc
      541 gtcatcgacg aagagcgcgc cttacatcaa gacatccgtg ccttgcgtgt ggcaattctg
      601 aatctaatgc caaccaaggt cgctacagaa acacagattc tgcgcttact ttcaaactct
      661 ccgttgcaag tggagatcac actagttcac accgctaccc acgaatccaa gaacacagat
      721 gcagaacatc tgctgcaaca ttatgtcacc tttgaagata tcaagcatga aaaattcgat
      781 gggctcatta tcacgggtgc gccagtggaa caactcccct ttgaagaagt agattactgg
      841 gaagaactca aacaggtgat ggattgggca aaaaccaata ccgagtccac attttatatt
      901 tgttggggag cccaagccgg actctatcat tactacggcg tccccaaata tgacctaccg
      961 caaaaaatgt tcggcgtttt tgaacaccgc ctcttaaacc ggaccgaaag cctgatgcgc
     1021 ggctttgacg acattttcct cgctccacac tcccgccata ctgagattcg ccgcgaagat
     1081 attgaaaagg tgcctgaatt acagatcctc gccgaatcag atgatgcagg tgtttacatc
     1141 gtcggttcaa gagatggacg tgagctcttt attacgggtc actctgaata cgaccctctc
     1201 acacttaaag cagaatacga ccgtgatgtg aacaaggggt tacctattca cgttccgaaa
     1261 aattattatc caaacgatga ccccaagcaa atgccaaatg tgcgctggcg cgggcatgcg
     1321 aatttactgt acgccaactg gttgaactat tacgtatacc aagcaactcc ctttgaccca
     1381 aatgaaattc caaagcggaa ctaatgactt caaaacataa cttcggcttc accactaggc
     1441 aaatccacga aggctacact ccagaccccg ccaccggcag tcgggctgtt ccgatctacc
     1501 aaaccacagc ctacgacctg caaagcacag accgtgccgc aagactcttt gcccttcaag
     1561 aaaacggtaa tatctacacc cgcatctcaa acccaaccag caacgtcttc gagaagcgcg
     1621 tcgccagcct cgagggcgga atcggcgcgc ttgaagttgg ctctggtcat gccgcgcaag
     1681 ctaccgccat cctcactatt tgcgaagccg gtgaccatat cgtctccacc tccctgctgt
     1741 atggcggaac ggtcacccaa ttcaaataca cattcccaaa acttggaatc accgtcactc
     1801 tcgtagaccc aagcgacccg gaaaactttc gtcgcgccat tcagccaaat acaaaaataa
     1861 tctacggcga aaccatcagc aacccgctcg gcaatatctt tccattcgaa gaaatctcca
     1921 agatcgcaca cgaacatggc atcccgctca tgatcgataa taccttcgcc acgccatact
     1981 tgtgtcgtcc cttcgaatgg ggtgcagata tcgtcaccca ctccaccacc aaattcatcg
     2041 gcgggcacgg taccaccatg ggcggaatta tcgtagatag tggcaaattc aattggagta
     2101 agagcgggcg cttcaaaaac ttcaccgagc ctgatcctgc ataccatcat gttatctacg
     2161 ctgatgactt tggtccattg gcgttcatct ccaaagcacg cgctggcatc ttacgagact
     2221 taggcaccgt ccaacagcca attgcatcct ggtttcttat tcaagggtta gaaaccttat
     2281 cgttgcgaat gacacgtcac gtgcaaaatg cgaaacgagc cgccgagttt cttgagaatc
     2341 atcccaatgt gaaatgggtt tcatacgcag gtttacccag tcacccagac catgaacgcg
     2401 ccaagaaata tttaccccaa ggcacaggcg ctgtactcgg gtttggagtc aaaggcgggc
     2461 gtgaagcagg agcgaagttc atcgacaacc tgcagctctt cagccatgtc gccaacattg
     2521 gtgacacgcg ctcactggca attcatcccg ccagcaccac ccacagccaa ctctccgacg
     2581 aagagcaaac cgccgcaggc gtcagccctg atttcattcg cctctctatt ggactagaag
     2641 atatcgaaga tattctctgg gatctggatc aggcgctttc attaatatag aagctaaaat
     2701 aatgaatgcg gattaaaatt ctgcattcat tatttcaaat gcccaaaatc accctacgct
     2761 ccgtcctccc ctctgaccta cccatcctct tccagcaaca actcgaccca gaagccgttg
     2821 ccatgtctgc ctatcctgcc aaggaccgtg gcgagttcat gcggcattgg gaaggcatca
     2881 tgaaaaataa aaatgtgatc gcgcgaacca ttgtctataa agagaaggtt gcgggtcaca
     2941 tcatttgttg gaaagagggc aagtatgaac aaagaatcgg ctattggatc ggcaaagagt
     3001 tttggaggcg gggcattgcc agtactgc
//
LOCUS       DAOQJE010000415         3024 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_21845_length_3024_cov_7.548670, whole genome shotgun sequence.
ACCESSION   DAOQJE010000415 DAOQJE010000000
VERSION     DAOQJE010000415.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3024)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3024)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3024
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21845_length_3024_cov_7.548670"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1409)
                     /locus_tag="PLT08_11035"
     CDS             complement(<1..1409)
                     /locus_tag="PLT08_11035"
                     /inference="COORDINATES: protein motif:HMM:NF012869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNE05044.1"
                     /translation="MTSLSVPAEFTLQRKNKYDQSTTLRWILSHVQPYWWIVAMIFIG
                     AIGNAVLAVVIPVLTGDAFNAMLAPKPDTSVLLPLAVTIGISQIIRGVLQLGRNFGAE
                     LMAQKMERDIRDELYLSLLGKSMTFHNLQPVGDTMARSTNDVREVNYMFSPGVNLVVG
                     SFMFILMPLFFGPRYHPALVLTPLLFTIFYFIALVRYLKTLSPITDDVRATFGQMNTH
                     LSESLDGVEIMKGAAQEEAEVDRFVTNARKVRDAFVQQGDLEARYIAMLLLGLAFAGG
                     LVHALFLLRQGLINVGAVIGYFGMLQLLGFPTFTSTFAYSQISLGFSSARRILELIQR
                     ETKLDQNASGMTSEIRGEIEFRNVSFHYPTPAEGGAGGDNVLENISFKVKPGQTVALV
                     GQTGAGKTTLVKLINRIYDVTDGQVLVDGVDVREWNLASLRSQISIIEQDIFLFSRTL
                     SDNIAFGKPGATKEEIIAAS"
     gene            1522..1671
                     /locus_tag="PLT08_11040"
     CDS             1522..1671
                     /locus_tag="PLT08_11040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE05045.1"
                     /translation="MKVSLPKTISGWCMWLFFLCFGLATFVPVLGTITPWLALAYAVL
                     SLLGM"
     gene            complement(1713..2603)
                     /locus_tag="PLT08_11045"
     CDS             complement(1713..2603)
                     /locus_tag="PLT08_11045"
                     /inference="COORDINATES: protein motif:HMM:NF016533.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA];
                     GO_process: GO:0019748 - secondary metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside phosphotransferase family
                     protein"
                     /protein_id="HNE05046.1"
                     /translation="MQFPEPFLSNIKHSFGEAGEIFLASLPALIEEASQRWQLTDIQP
                     VSNLSYNFVAFAQRGRDKVMLKIGVPHRELISEMAALILFNGDGACQIIDCDEGKGFL
                     LLEQLQPGKMLADLEDDDERTRIAIEVMKKIWRPAPETGKFIQLEDWFAELENIRPAF
                     DGTTGPYPKELLEKVESFLPELFKDEHYLIHGDFHHFNILSSERGWLAIDPKGVIGPC
                     GYEVGPLMLNPWNNSMDSAQFEVRAKRRVDLLHEGLGWEREQIIQWSTAHALLSSWWD
                     YPNGDWQCSLRCANIFAGLK"
     gene            complement(2605..>3024)
                     /locus_tag="PLT08_11050"
     CDS             complement(2605..>3024)
                     /locus_tag="PLT08_11050"
                     /inference="COORDINATES: protein motif:HMM:NF025687.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4386 domain-containing protein"
                     /protein_id="HNE05047.1"
                     /translation="VIGFIVQVVGLLRWVFVVPALARLYTDPATDSATQAAVAAVFTA
                     VHQYGGVILGEHMGQFLIILWMSMISGIMYKSKMFSKWVAILGWVASAIYLLAQTELL
                     ATAIPTFPVIDWAGLVGSLLWLVWMIVMGVYLVKYKE"
BASE COUNT          815 a          744 c          739 g          726 t
ORIGIN      
        1 gatgcagcaa tgatttcctc ttttgtcgca ccgggtttgc caaaggcgat attgtcgctt
       61 agggtgcgcg agaagaggaa gatatcttgt tcaatgatgg atatctgaga acgcagcgat
      121 gccagattcc attcgcgcac gtctacacca tcaacaagca cttgcccatc tgtcacatca
      181 tagatgcggt tgatgagctt gacgagggtg gtcttgccag caccggtctg cccgacgagt
      241 gccacggttt gaccgggttt cactttgaat gaaatattct caagtacatt atctccaccg
      301 gcaccgcctt cagcaggtgt agggtagtgg aacgagacgt tgcgaaactc aatttcgccg
      361 cgaatctccg aggtcatgcc tgaggcgttt tggtcaagtt ttgtctcgcg ttgaatgagt
      421 tccaagatac ggcgagcgga tgaaaatccc aaagagattt gagagtaggc aaaggttgag
      481 gtgaaagtgg gaaagcccaa gagttgcagc atgccaaaat agccaatcac ggctccaacg
      541 ttgattagcc cttggcgcag caaaaacaat gcgtggacaa gcccgcctgc gaaagccaga
      601 ccgagtaaga gcatggcaat gtaacgcgct tcaaggtcac cttgctgcac aaaagcatca
      661 cgaactttgc gggcgttggt cacgaaacgg tccacttcgg cttcttcttg cgccgcacct
      721 ttcatgatct cgaccccatc aagtgactca gatagatgcg tgttcatctg tccaaacgtt
      781 gcacgcacat catcggtaat aggcgaaagc gttttgagat atcgaaccaa tgcgatgaaa
      841 tagaaaatgg taaatagcag cggggtgagt accaacgctg gatgatagcg cggaccaaag
      901 aacagtggca tgaggatgaa catgaacgaa ccgaccacaa gattcacgcc ggggctaaac
      961 atgtaattca cttcgcgcac atcgttcgta gagcgtgcca ttgtatcgcc cacaggttgt
     1021 aagttatgga aggtcatact cttgccaagc agcgagagat acaactcatc gcggatgtct
     1081 cgttccattt tttgtgccat caactcggca ccaaagttac gccccaattg cagcacacca
     1141 cggatgatct gtgaaatgcc gatcgttaca gccaacggca gaagcacaga ggtatctggt
     1201 ttgggcgcaa gcatggcatt gaatgcatca ccggtcaaca ccggaatgac aacagccaat
     1261 acggcgttgc caattgcacc aatgaagatc atggctacga tccaccaata aggctgtaca
     1321 tgagaaagaa tccatcgcaa tgttgtggat tgatcgtact tattctttct ctgtaaagta
     1381 aattctgctg gaacacttag ggaagtcata aagtaattgt aacatcccta aaaaagcgca
     1441 ccgcaaccct aactttagta aggtgtttta ttttgtagct ggcaattctg ccagcaccaa
     1501 caatttatag gagagtgtga aatgaaagtt tctttgccca aaacgatttc tggttggtgt
     1561 atgtggctgt tcttcttgtg ctttggtctc gcaacattcg tgcccgtatt aggcaccatc
     1621 actccctggc tggctcttgc ctacgctgtc ttaagccttc ttggtatgta actcaattta
     1681 taaacacaca acctcccgaa agggaggttg tgttatttaa gtcctgcaaa aatattggca
     1741 caccgtaacg agcattgcca atcgccattg ggataatccc accaggagga taagagggca
     1801 tgggctgtgg accattggat gatctgctcg cgctcccagc ctaacccttc atggagcaag
     1861 tccacccgcc tctttgcgcg gacctcgaac tgggcactgt ccattgagtt attccacggg
     1921 ttgagcatga gcggaccgac ctcatagcca caaggaccaa tcacaccctt agggtcaatg
     1981 gcgagccaac cacgttctga agagagaatg ttgaaatgat gaaaatcacc atgaatcaaa
     2041 taatgctcat ctttgaataa ttccggtaga aatgattcaa ccttctccaa caattcttta
     2101 gggtatggtc cggttgttcc atcgaaagcg gggcggatgt tttctaactc tgcaaaccaa
     2161 tcttccaact gaataaactt tccggtttca ggtgcagggc gccagatttt tttcatgact
     2221 tctattgcaa tgcgagtccg ctcgtcatca tcttccaagt ctgccagcat tttgcctggc
     2281 tgaagttgtt caagtaataa aaatcccttt ccttcatcac aatcaatgat ctgacaggct
     2341 ccatcaccgt tgaacaatat cagcgctgcc atttcgctga ttagttcgcg gtgtggcaca
     2401 ccgatcttga gcatcacttt atcacgtcca cgctgcgcaa aggcgacgaa gttatacgag
     2461 agattgctca caggctgaat atcggtcaac tgccagcgtt gtgatgcttc ttcgatcaaa
     2521 gcagggaggc tggcaagaaa tatctcgcca gcctcgccaa aagaatgttt gatgtttgag
     2581 agaaagggtt cagggaattg catactattc cttatatttc accaaatata ctcccatcac
     2641 gatcatccag accagccaaa gtaagctgcc aaccagtccc gcccaatcga taacggggaa
     2701 agtgggaata gcggtggcga gaagttccgt ctgcgcgagg aggtaaatgg cagaggcaac
     2761 ccatcccaga atggcaaccc atttgctaaa catcttggat ttgtacataa tgccgctgat
     2821 catagacatc cacaggatga taaggaattg tcccatatgt tcgccgagga tgactccgcc
     2881 atattggtgg acggcagtga acacagcggc gactgcggct tgggtagctg aatccgttgc
     2941 cgggtccgtg tagaggcggg cgagggcagg cacgacgaag acccagcgca agagtcccac
     3001 cacctggacg atgaagccga tcac
//
LOCUS       DAOQJE010000416         3022 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_21867_length_3022_cov_4.317829, whole genome shotgun sequence.
ACCESSION   DAOQJE010000416 DAOQJE010000000
VERSION     DAOQJE010000416.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3022)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3022)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3022
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21867_length_3022_cov_4.317829"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(152..952)
                     /locus_tag="PLT08_11055"
     CDS             complement(152..952)
                     /locus_tag="PLT08_11055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE05048.1"
                     /translation="MKNKTVIWATISLLFLVVFGIYIILENATYLDWGEYKVPEYPVG
                     GSDSVSIYTINSQTILSSLEAGSKSIFTQRPLVVEDSTMIWQYGSLTWKQDDYINIAT
                     RFHQFVWGETLEDWLVYNATFYIYNYPKPGGIEYAHLVFYKRQNNEYIVHTITIKPLY
                     NQIIAGSTIYHDEDKWRGFDLNKLAIRNVDDALLIADENGGHELCRDNKSHCSVGIWL
                     DSTLNESNIWGLIPLYTYEWSWNVTYYDESQNHIFDIKIDPFTGKIIE"
     gene            complement(974..1651)
                     /locus_tag="PLT08_11060"
     CDS             complement(974..1651)
                     /locus_tag="PLT08_11060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE05049.1"
                     /translation="MDEINTYTAAGIGVQCFGTDIKDPRRIGDWDNSGEGPAQITDNE
                     RKTPYGERIGTRETPRGWGLLCWIKKNAGLDAGCECLTEDEMSNKYGEDFRNEFELEK
                     TRDQHEPTWAGIFMRRRIDLVVGKCKNCSAVDKFIIAGLAQNGPGFNVDTMTDVINPN
                     YNPTGRDIDWIQWYENGVESPKSRKEYPHQLRIFHSVILKLKADNYYLPADLNLYDPE
                     ILYLMNR"
     gene            2114..2401
                     /locus_tag="PLT08_11065"
     CDS             2114..2401
                     /locus_tag="PLT08_11065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE05050.1"
                     /translation="MQFVPYRDLRNEPSTLRKKLTSEGELVVTVDNKPFAVMINLEDE
                     NVQDVLLMVTRLRAQMAARSIRSQARRDGLDKMSLKDVNAIIKKSRATRKR"
     gene            2401..2820
                     /locus_tag="PLT08_11070"
     CDS             2401..2820
                     /locus_tag="PLT08_11070"
                     /inference="COORDINATES: protein motif:HMM:NF024861.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - endoribonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative toxin-antitoxin system toxin component,
                     PIN family"
                     /protein_id="HNE05051.1"
                     /translation="MLIIVLDTNVLVSSLLSSRGAPSKILDLILGEQVIVAYDNRILG
                     EYEEVLSRPEFKIHPSKVFAIVNQIEISGRYIETEPLSSEGFTDPDDITFAEVFITSE
                     ADALVTGNLKHYKPLLGKNMLVLTPAQFLDKFFPKQD"
BASE COUNT          865 a          630 c          583 g          944 t
ORIGIN      
        1 ttgaacgcgg atactggcag atcagccaat cactgaatgg ggtgaggttg aggtgcatga
       61 taatctcctt gttggtgggt ttacgggaga ttattatgta ttctgtacta atggattatt
      121 tcagtttgcc aagtatgaaa tactattatt tttattcaat aattttccca gtgaacggat
      181 ctattttgat atcaaaaata tgattttgcg attcgtcgta gtaagtaacg ttccaactcc
      241 attcataggt atataaagga atgagacccc agatattact ttcatttaaa gttgaatcaa
      301 gccatattcc aacgctacaa tggcttttat tatcccgaca taattcatgc ccgccattct
      361 catcggcaat taacaatgca tcatcgacat ttctaattgc aagcttgttt aggtcaaaac
      421 ccctccattt atcttcatca tgataaattg tgctacccgc gattatttga ttataaagag
      481 gtttaatggt aattgtatgg actatatatt cattattttg acgcttgtag aaaactaggt
      541 gagcatattc aattcctcct ggttttggat aattatagat ataaaaggtc gcattataca
      601 caagccaatc ttccaaggtt tcaccccaga caaactgatg aaacctcgta gctatattaa
      661 tataatcatc ctgtttccac gtcagcgacc catattgcca aatcatggtt gaatcttcaa
      721 ccactaatgg tctttgtgta aatatacttt ttgaccctgc ttcaagagat gaaagtattg
      781 tctgtgagtt gatcgtataa attgacacac tatcagatcc gccaacaggg tattctggta
      841 ctttgtattc tccccaatcc aaatacgttg cattctcaag tattatataa ataccaaata
      901 caacaagaaa tagaagtgaa atagtagccc atattacggt tttatttttc attgctcatc
      961 aatccgctat ttattaccta ttcatcaaat acagtatttc agggtcatat aaattgagat
     1021 cagcaggtag ataataatta tctgccttta attttaagat aacactatga aaaattcgta
     1081 attgatgggg gtattctttt cgagactttg ggctttctac cccattttcg taccattgaa
     1141 tccaatcaat gtcacgacca gtaggattgt aattaggatt aatgacatca gtcattgtat
     1201 caacattgaa gcccggtcca ttttgagcca atcctgcaat gataaatttg tctacggcgc
     1261 tacagttttt acatttgcca actaccaggt ctattcttct tctcataaaa atcccagccc
     1321 atgtaggttc atgttggtct cgagttttct ctaattcgaa ttcatttctg aaatcctcac
     1381 catatttatt ggacatttca tcttcggtga gacactcgca tccagcatct aaacctgcat
     1441 tcttcttaat ccaacagagc aatccccaac ctcggggcgt ttctcttgtt cctattcttt
     1501 cgccatacgg agttttcctc tcattatcag ttatttgtgc tggaccctcg ccactattgt
     1561 cccaatcacc tattctccgt gggtccttta tatctgtacc aaaacattga acaccaatac
     1621 cagcggctgt atatgtatta atctcgtcca ttatgagatt cccgctagag tctttgttgc
     1681 tcacatacat tttagcgaaa ttagcacggt ttgtgggtgt tccccaaaaa catctctgag
     1741 gtttaatggg atagtgacca gttgggtcaa tcaaaatcac cggattcccc tctgtgtatc
     1801 cccaccgatt tagggatagg gggcggttgt aatctccact ccacgtatcg cgtgattgga
     1861 acctcccatt tgtggggttg taatatctgg ctctgagata aattaactgt gtggaatccc
     1921 catattgttc agcttttttt ctatacacca caatcgcaag ctataaagac atttcaagag
     1981 aaaaaactct cttgaagatt ggttctttag aataacttgg ttctttttaa gaattttggt
     2041 tttcatgcaa ttgaccgaag ataggaagtc gggtaagata gtgtagcaaa gtatgctacg
     2101 aaaggagcac atcatgcaat tcgtacccta tcgagatctg cgaaacgagc catccactct
     2161 ccgaaaaaag ctgacaagtg agggggaact agtcgtgaca gttgataata aaccatttgc
     2221 tgtaatgatc aatttggaag atgagaatgt acaagatgtt cttttgatgg taacacgtct
     2281 gcgagcgcaa atggctgcac gatcaatccg cagtcaggca cgcagggatg gattggacaa
     2341 aatgtctctc aaggatgtca atgctatcat taagaaatct agggcgactc ggaaacgctg
     2401 atgctgataa ttgtcttgga taccaacgtc ttagtcagca gtttgttgag ttcgagaggc
     2461 gcaccctcaa aaatccttga cctaattcta ggggagcagg ttattgtggc gtatgacaat
     2521 cgcattcttg gtgagtacga agaagttttg tctcggccag aattcaagat tcatccttcc
     2581 aaggtgtttg caatcgtaaa tcaaatcgag atttcaggac gttacataga gacggaaccg
     2641 ctttcctctg aaggattcac cgacccggac gacataacgt tcgctgaggt tttcattact
     2701 tcggaggcag atgcgttggt tacaggaaac ttgaaacatt acaagccatt acttgggaag
     2761 aatatgcttg ttcttactcc tgctcagttt ctggataaat tcttcccaaa acaggattaa
     2821 ttcgccacga ataaacatat cctagagtcc ataggtaaag aagattttaa tcatgcaacc
     2881 gccaccctgc taagctgaac aataagggga ttccacacag ttgctgttcc tgcgggcgag
     2941 atattacaat cctgccgttg ggaggttcca atcactggat acgtggagtg gagattacaa
     3001 ccgtccgctt tcgctgaacc gg
//
LOCUS       DAOQJE010000417         3020 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_21896_length_3020_cov_3.247892, whole genome shotgun sequence.
ACCESSION   DAOQJE010000417 DAOQJE010000000
VERSION     DAOQJE010000417.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3020)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3020)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3020
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21896_length_3020_cov_3.247892"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..234
                     /locus_tag="PLT08_11075"
     CDS             <1..234
                     /locus_tag="PLT08_11075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE05052.1"
                     /translation="EVLKPYSNIFMLEDHHPAGGLMDNILTACSENGLLNGKQMIKFA
                     VEGYPACGTPQQALAFHELDGLSLSKRVLKYCG"
     gene            240..1658
                     /locus_tag="PLT08_11080"
     CDS             240..1658
                     /locus_tag="PLT08_11080"
                     /inference="COORDINATES: protein motif:HMM:NF016357.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0047355 - CDP-glycerol
                     glycerophosphotransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-glycerol glycerophosphotransferase family
                     protein"
                     /protein_id="HNE05053.1"
                     /translation="MENIKIGIPLHGKMSSRTFHSALFRERLGEKGFEPIYFLTSDYL
                     EASPYDPKRYFELKVSAYEAYYEKHALLRNMKSLRRFVIVTETTDLRLREEIESIIFQ
                     KDLWKIGGYILYTSILRWVPGMGDFLLGLENLLYKTDAHTEVIRNIPLNCVLTPGMGN
                     YRFEYSGQFSLEAQRLGIPVFSAVTNYDNIVNMGFRGFTPKCIAVWSQQMADELMRLH
                     KMSGKRIEVTGPIQYDRFKQPLPRSREEFLESIGLDPKKKTIFIAGGVNITRYFELFN
                     LFFEQQTNVFSEPCNIILRPYPHVKLLSSPGWKILRKLFLDRGVYISIPDAIDSHEHR
                     SQELKSDLYSEEEHDELNYLLRYSDVMVNYFSTISLEAAICDLPVIHVGYDIYTYGHR
                     FHVTSEFLQRQTHNRRKFRLAASRVAKTEKDLVKYINMYLSDKSLDRDARYEYAISEC
                     GELDGHAGRRIVQMIKSRLQRQ"
     gene            1677..2327
                     /locus_tag="PLT08_11085"
     CDS             1677..2327
                     /locus_tag="PLT08_11085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE05054.1"
                     /translation="MQKLVQEIKKSTHLLPPLQRWIDHQEQAADLAYWQMKPRTLPLP
                     HLLKQDILKGYAIEYKLPVLIETGTYMGEMVEALRNVFKTIYSIELSDKFAQRAKKYF
                     RYSSNVTILQGDSGEVLRELIPKIDQPALFWLDGHWSADQTARGKKSTPIMEELDHIL
                     HAKDLGHVILIDDARAFGKSEDYPTLDTLSDFVLSLRKNVKIISEDDIIRILPQYE"
     gene            2320..>3020
                     /locus_tag="PLT08_11090"
     CDS             2320..>3020
                     /locus_tag="PLT08_11090"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family A protein"
                     /protein_id="HNE05055.1"
                     /translation="MNKPSLLTIGILSWNRLNYLRATLESAKRCIQYPNIQWIVLDNY
                     STEPGLAEYLKSLAWIDELIFMQSSHVAAMNEIVARAKGDVLLLWPEDVQFVVEGDWM
                     RDCIEILLKDYSIGALSLNFHRRQTIEYIWGRQRFNKNGILRMWKDLKRYGANFRLQK
                     KNISSNGYPVRTFGWQEDGIIGAGITSLARTEVWKKLGPWKAKPADDNIIDSSGGGET
                     EMLERWAKTKTPLQRA"
BASE COUNT          916 a          541 c          673 g          890 t
ORIGIN      
        1 gaagtcctca agccttactc aaatatcttc atgctcgaag atcatcaccc cgctggtggt
       61 ttgatggata atattctcac ggcttgcagt gagaatggtt tgctcaatgg caagcagatg
      121 atcaagtttg cggtggaagg ttatcctgcc tgcggtacgc cgcaacaggc gttggcgttc
      181 catgaattgg atggattatc cttgtctaag cgtgtgctaa aatactgcgg ataaagtaaa
      241 tggaaaatat aaaaattgga atacctctgc acggaaagat gtcttctcgc acctttcatt
      301 ctgcattatt tcgagaacgc cttggtgaaa aaggttttga accgatttac ttccttacct
      361 ctgactacct tgaagcctct ccctatgacc cgaagcgata cttcgagttg aaggtttcag
      421 cctatgaggc atattacgaa aagcatgcat tgttgcgcaa catgaaatct ctaagacgct
      481 tcgtgattgt gaccgagaca acagatttgc ggctgcgaga ggaaatagaa agtattattt
      541 ttcaaaaaga tctttggaaa atcggtggat acattcttta tacaagtatt ttgagatggg
      601 tgcctgggat gggggatttt ctgttggggc tggaaaatct attgtataaa actgatgcac
      661 atacagaagt gatcagaaat attccattga actgtgtcct aacgccgggt atgggaaatt
      721 atcgatttga atattcagga caattttcgc ttgaggcaca gcggttggga ataccggttt
      781 tcagcgctgt tacaaattac gataatatcg ttaatatggg atttcgtggt tttactccaa
      841 aatgcatagc ggtttggagc cagcaaatgg cagacgagct catgcgttta cataagatgt
      901 ctggtaagcg catagaagtg acaggtccga ttcagtatga ccgttttaag caaccacttc
      961 ctcgcagccg ggaagaattt ctcgaatcta ttgggcttga cccaaagaaa aaaaccattt
     1021 ttattgcagg tggcgtaaac attactcgct attttgagtt atttaatctc tttttcgagc
     1081 agcagacaaa tgttttttca gaaccttgta atattattct gcgcccttat ccacatgtga
     1141 aattgctttc ttcacctggt tggaagattt taaggaagtt attcctggac agaggagttt
     1201 acatctcaat cccagatgcg attgattcgc atgaacatag gtcgcaagag cttaagagtg
     1261 atctctattc tgaagaagaa catgatgaat tgaattacct tctgcgttac agcgatgtaa
     1321 tggtgaacta tttttcgacc atcagcctag aagctgctat atgtgattta cctgtgatcc
     1381 atgtaggtta cgatatctat acatatggac atcgttttca tgtaacatct gaatttttac
     1441 aacgacagac tcataatcga agaaaattca ggcttgcagc atcacgtgtt gctaaaactg
     1501 aaaaagattt ggtaaagtat attaatatgt atcttagtga taagtcgtta gatcgagatg
     1561 cacggtatga atatgctatt tccgagtgtg gtgaattgga tgggcacgca gggaggcgaa
     1621 ttgtgcaaat gattaagagc cgtttacaac gccagtgagg aactaatatg aatgtaatgc
     1681 agaaacttgt acaagaaatt aaaaaaagta cacatctact tcccccactt caaagatgga
     1741 tagaccatca ggaacaagcg gcagatcttg cttactggca aatgaaacct cgtacccttc
     1801 ctttgccaca tctcctaaaa caagatatct tgaagggtta tgcgattgaa tataaattac
     1861 ctgttttgat tgagacaggt acatacatgg gcgagatggt tgaagcttta cgtaacgtat
     1921 tcaaaaccat atattcgatt gagttaagtg ataaatttgc acagcgcgca aaaaaatatt
     1981 ttcgctattc cagtaatgtg actattttgc aaggtgacag tggagaggtt ctaagagaat
     2041 tgattcctaa aatcgaccag ccggcccttt tttggctgga tggacattgg tctgcagacc
     2101 aaactgctcg tgggaaaaaa agtacaccta ttatggagga attggaccat atcctgcatg
     2161 ctaaagattt ggggcatgta attctaatag atgatgctcg tgcttttgga aaatctgaag
     2221 actatccgac cttggataca ttaagtgatt ttgtcctttc gctacgtaag aatgttaaaa
     2281 taatttcgga agatgacatt ataagaattt tgcctcagta tgaataaacc atctttatta
     2341 accattggta ttttaagttg gaaccgcttg aattatttac gtgcaacgct tgagtctgct
     2401 aagcgttgta ttcagtaccc gaatatccaa tggattgttt tggataatta ttcaacagaa
     2461 cctggtttag ctgaatactt aaaaagcctt gcctggattg acgagctaat atttatgcaa
     2521 tccagtcatg ttgctgctat gaatgaaatc gttgctcggg caaaagggga tgtcctttta
     2581 ttatggcccg aagatgtgca atttgttgtg gaaggtgatt ggatgaggga ttgcattgaa
     2641 atattactaa aagactattc tattggtgcg cttagcttaa attttcaccg ccgccaaacg
     2701 atagaataca tttggggaag gcaacgcttt aataaaaacg gtatcttaag aatgtggaaa
     2761 gacctgaaac gatatggtgc taattttcgc cttcaaaaaa aaaatatttc ttcaaatggg
     2821 tatccagtgc gaacgtttgg ttggcaagaa gatggaatca ttggtgcagg tatcacatct
     2881 cttgcccgca cagaagtatg gaagaaactt ggcccatgga aggcaaaacc tgctgatgac
     2941 aatattatag actcaagcgg cggtggggaa actgaaatgt tagaacgttg ggcaaaaaca
     3001 aaaacaccct tacaaagagc
//
LOCUS       DAOQJE010000418         3019 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_21906_length_3019_cov_4.621120, whole genome shotgun sequence.
ACCESSION   DAOQJE010000418 DAOQJE010000000
VERSION     DAOQJE010000418.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3019)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3019)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3019
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21906_length_3019_cov_4.621120"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..591
                     /locus_tag="PLT08_11095"
     CDS             <1..591
                     /locus_tag="PLT08_11095"
                     /inference="COORDINATES: protein motif:HMM:NF024245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComEA family DNA-binding protein"
                     /protein_id="HNE05056.1"
                     /translation="MKNVLYTLVGVMAGFALAGLLLFISRAPAGEPIKLLPAPTKEPI
                     AVHVVGAVPRPGLYEFAEGSRVQDAIDAAGGLLASASVDTINLAALLEDGQQLNIPFK
                     AGEEPSTSNSSDDTSLVLPGATEASSSGSSQDLININTASVEELDSLPGIGPTIAQRI
                     IDYRDENGPFQTIEDILNVSGVGPSTFDQIKDLITT"
     gene            591..1139
                     /locus_tag="PLT08_11100"
     CDS             591..1139
                     /locus_tag="PLT08_11100"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNE05057.1"
                     /translation="MGFDHFSAIAPIYARVTYSKKEMMREVAFLPVQGRLLDVGGGTG
                     RVSSAIREYVDEVIIADVSFGMLARAPRPSLQPVCGASETLPFADNFFERVIMVDALH
                     HVANHAHTAHEMFRVLKPGGVLVIEEPDIRTFGVKLIALAEKLLLMRSHFLAPDGIMK
                     LFDIGEKETKSVNGTAWVIIKK"
     gene            complement(1173..2321)
                     /locus_tag="PLT08_11105"
     CDS             complement(1173..2321)
                     /locus_tag="PLT08_11105"
                     /inference="COORDINATES: protein motif:HMM:NF012656.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HNE05058.1"
                     /translation="MNFDLTEEQKTWKKIVHDFAEKELRPKAREVDEKEEFNWEAFRK
                     AGPVGLASMSIPEEYGGSNVDMISNAIAFEELGWGCGSTALAFTAHNGLGTAPIALYG
                     SEELKKKWLPLVGNGKNKLGALALTEPGAGSDIQGGVTTRAEKDGDDWVINGAKMWNT
                     NASIAEYIITLVRTDPKGGSKSLSMILVPTDSKGLSIAPAEKKMGLRGSPTHAVTFDN
                     VRVPLGNLIGPEGRGLQQTLSTLDAGRISIGAISLGLARAGMEAAIQYSKERKAFNQS
                     LADFQAIQFKIANMEMEIELARTYIYKAAWLKDEGRPYSKEAAIAKLYATEMAERVLY
                     DAIQIHGGYGYSREYNVERMYRDARLMTIGEGTSEIQRLVISRHVLAG"
     gene            complement(2335..2679)
                     /locus_tag="PLT08_11110"
     CDS             complement(2335..2679)
                     /locus_tag="PLT08_11110"
                     /inference="COORDINATES: protein motif:HMM:NF014672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE family protein"
                     /protein_id="HNE05059.1"
                     /translation="MKNTVLLCTKFGMGQTDIASLNQKLISKYLSLLLEGNQLPAAIC
                     FYTDGVKLVTGISPVLEQLSQLEQKGVRLIICSTCLDEMGISRDDVKVGIVGGMPDII
                     EAQTKADKVITL"
     gene            complement(2683..>3019)
                     /locus_tag="PLT08_11115"
     CDS             complement(2683..>3019)
                     /locus_tag="PLT08_11115"
                     /inference="COORDINATES: protein motif:HMM:NF020967.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF2007 domain-containing protein"
                     /protein_id="HNE05060.1"
                     /translation="HPSICGLEAYPNWYAPISCERSMDCIIYSVLEIKMDELKYDLLT
                     ELTNRMQADLLESFLEANGIDVELFQESVGHSIYPVTIDLLGRVQVFVPKDKIEQAKI
                     LLEEFNAEE"
BASE COUNT          742 a          709 c          670 g          898 t
ORIGIN      
        1 atgaaaaatg tgctttatac gttggtgggt gtcatggctg gttttgcatt agcaggtttg
       61 cttttgttta tctcacgcgc gcctgctggg gagccaatca agttattgcc tgcaccaacc
      121 aaagaaccga ttgcagttca tgttgttggt gctgtgccgc gcccgggttt atatgagttt
      181 gcggaaggct cccgtgtgca agatgcaatc gatgctgccg gtgggcttct cgcaagtgcc
      241 agtgttgata cgattaactt ggctgcattg cttgaagatg ggcaacaact caatattcca
      301 tttaaggcgg gtgaagaacc ctcaacttca aattctagtg acgatacttc ccttgtgctt
      361 cctggtgcaa ctgaagcaag ctcttctgga tcttctcaag atttgattaa catcaatacg
      421 gcatctgtcg aagagttaga tagccttccg ggtattggtc caaccatcgc tcaacgcatc
      481 attgattatc gtgatgagaa cggtccattc caaacgattg aagatatttt gaacgtctct
      541 ggggtaggtc cttctacatt tgaccaaatc aaggatttga taacaaccta atgggttttg
      601 atcacttttc tgccattgct ccgatttatg cgcgtgtcac atactctaaa aaagaaatga
      661 tgcgcgaagt tgcttttctt ccagttcaag gacgtttgct ggatgtaggc ggtggcaccg
      721 gacgtgtttc ttctgccatt cgtgagtatg tggatgaggt catcattgcc gatgtttcat
      781 ttggtatgtt agcccgtgcg cctcgacctt cccttcagcc tgtgtgtggt gcttctgaaa
      841 cattaccctt cgctgataac ttttttgaac gggtcatcat ggtggatgct ttgcatcatg
      901 tggcgaatca tgctcatact gctcatgaaa tgtttcgtgt gctaaagcct ggtggcgtgc
      961 tcgtcatcga agaaccggat atacgtacct ttggggtgaa gttaattgct ttagcagaga
     1021 aactgctgct catgcgcagt cacttcctcg cgccagatgg aattatgaaa ttatttgata
     1081 taggggaaaa agaaaccaaa tcggtgaatg gaacagcttg ggtcatcatc aaaaaataaa
     1141 aaagcggagg catggaagcc tccgcttttt tattatccag ctaacacatg ccttgaaatg
     1201 accagcctct gaatttcact ggtcccctcg ccaatggtca tgagacgtgc gtcgcgatac
     1261 attcgctcca cgttgtattc acggctatac ccatacccac catggatttg aatggcatcg
     1321 taaagcactc gttctgccat ttcagtagca tacaacttgg caattgcagc ttccttgcta
     1381 tatggacgtc cttcgtcctt gagccaggcg gctttgtaaa tgtacgttcg cgccagttcg
     1441 atttccattt ccatgtttgc aatcttgaat tggattgctt gaaaatcagc cagagactgg
     1501 ttgaacgctt tacgttcttt ggaatattga attgcagctt ccatgccagc gcgcgccaaa
     1561 ccaagcgaga ttgcgccaat gctgatgcgt cctgcatcca aagtggaaag agtctgttgt
     1621 aagcctctgc cttccggtcc aattaagttt ccaagcggaa cacgcacgtt gtcgaaggtc
     1681 actgcatggg taggggagcc gcgcaagccc attttctttt cggcaggcgc tatgctcaag
     1741 cctttagagt cggttggcac gaggatcatg ctcaacgatt ttgaaccgcc tttcgggtcg
     1801 gtgcggacga gggtgatgat gtattcggcg atactggcat tggtgttcca catctttgcg
     1861 ccattaatca cccagtcatc gccatctttc tcggcacgag tggtgacccc gccttgaata
     1921 tcagaaccag caccaggttc ggtgagagct aaggcgccaa gtttattctt tccgttgcct
     1981 accaaaggca gccatttttt ctttagttct tcactaccat acaaagcaat aggagctgtc
     2041 cctaagccat tgtgagccgt gaaagcaagt gccgttgagc cacaacccca gcctaattct
     2101 tcaaaggcaa tggcatttga gatcatgtct acattggagc cgccatattc ttcggggatg
     2161 ctcatacttg ccaaaccaac aggtccagcc ttgcggaacg cttcccaatt aaattcttct
     2221 ttttcatcca cttcacgtgc tttggggcgt aactcctttt cagcaaagtc atggacgatc
     2281 tttttccacg ttttttgttc ttcagttaga tcgaaattca taataactcc taatttatag
     2341 cgtaatgact ttatccgctt ttgtttgtgc ttcaattata tcgggcatcc cgcctacaat
     2401 gcctactttt acatcgtcac gagaaattcc catttcatca agacaggtag agcaaataat
     2461 aaggcgaacc cctttttgtt ccaattgaga aagttgctcc agtaccggag aaataccggt
     2521 tactagctta accccatctg tataaaaaca aatggcagca ggaagttgat tcccttccag
     2581 taacaggctc aaatacttgc taatcaattt ctgatttaga gaggcaatat ccgtttgccc
     2641 cattccaaat tttgtacaaa gtagaacggt gtttttcatt tcttattcct cagcattgaa
     2701 ttcttcaagc agtatttttg cctgctctat tttgtctttg ggtacaaaaa cctgcacgcg
     2761 ccctaataag tcgattgtga ccggatagat ggagtgcccc acagattctt gaaataactc
     2821 cacatcaatg ccattcgctt cgagaaaact ttccagtaaa tctgcttgca tgcgatttgt
     2881 gagttcggtt aataggtcat attttagttc gtccattttt atctccagaa cactataaat
     2941 aatgcagtcc atagaacgct cacaactaat tggcgcatac caatttggat aggcttccaa
     3001 cccacagatg gatgggtga
//
LOCUS       DAOQJE010000419         3018 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_21923_length_3018_cov_2.449544, whole genome shotgun sequence.
ACCESSION   DAOQJE010000419 DAOQJE010000000
VERSION     DAOQJE010000419.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3018)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3018)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3018
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21923_length_3018_cov_2.449544"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            169..849
                     /locus_tag="PLT08_11120"
     CDS             169..849
                     /locus_tag="PLT08_11120"
                     /EC_number="2.1.1.100"
                     /EC_number="2.1.1.334"
                     /inference="COORDINATES: protein motif:HMM:NF018649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenylcysteine carboxylmethyltransferase
                     family protein"
                     /protein_id="HNE05061.1"
                     /translation="MRSLPRYFLALLAGSIIFIGLPLLGWGLGNLPAFFENPARTAYV
                     VVIFILQIFSLIYNPQVGQNQENRKSDAPRNKLDLILIQIFSLGIVILAPFSDSHSFG
                     VLNIEVIVRYLGLLIAIPGFILMQAGEKHLARQFSIEVTLQKDHKLIQSGPYKVIRHP
                     RYLGILIFFTGIALTFRSLLGIFTVLALAGVLIWRVFTEEKMMHEEFGKEWEEYQIKT
                     WRLIPYIF"
     gene            complement(883..1698)
                     /gene="pheA"
                     /locus_tag="PLT08_11125"
     CDS             complement(883..1698)
                     /gene="pheA"
                     /locus_tag="PLT08_11125"
                     /EC_number="4.2.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004664 - prephenate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009094 - L-phenylalanine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HNE05062.1"
                     /translation="MKISFQGEPGAYSEQAVFNYYGKDVETVPCESFDVMFDSVISGK
                     SDAALAPIENSLAGSIHQNYDLLLRHNLHIVGEYFLRVRHCLIANPGVKKEDIKKAIS
                     HPQALGQCAAYLRNLGIKPEQVYDTAGSVKMLKESGAKDVAAIASKRAAELYGMQILE
                     EGIEDNQENFTRFLAVQREATIPTGDAKTSIVFTLKNVPGSLFKAMSVFALRDIDLTK
                     IESRPLQGKPWEYLFYIDLIGSTENETLQRALDHLGEYAVMLRVLGSYPRFKG"
     gene            complement(1734..2915)
                     /locus_tag="PLT08_11130"
     CDS             complement(1734..2915)
                     /locus_tag="PLT08_11130"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HNE05063.1"
                     /translation="MHNLNTQFDLIRQSATVAMADRILALKASGHKIVGLQVGDPDFG
                     THPAIAEAAFKAMQEGHTHYGPSAGTLELRKAIAAKLQRDEGVSYDPASEILVTHGGI
                     HAYYTAMQSILNPGDDVLIPDPSWATHSNMAIMLRGNVIRIPAPAENGFIPHFDSWLK
                     ALTPKTRVIVLNYPSNPTGAFPSREYLQKLQDFAKEHNLWIVSDEVYGSLYYEGKPTS
                     AAAIEGAKERTILVNSLSKSYAMTGWRVGFLAAPAPVIQNALKAGQNSITCVAPFIQK
                     AAAFALTDPKIQDVTAEMRAAYGRRRELVIRLSHELESDRVKIIPPQGAFYFFLDLRE
                     LKMSSVEMCEKILDEEGVGLVPGSAFGEQGEGFIRMTIAASEEDVEAGFRKIVNWAER
                     Q"
BASE COUNT          750 a          750 c          714 g          804 t
ORIGIN      
        1 aaatggcgcg gcggttgggg tctgcaatgg cttgaaatac gtctcgtctc ataattcctt
       61 tttgtaacca tttagttaca taatatagct agattttaga acaaattttc tgaattgtca
      121 agagggaatc atttatactt tcagcaaatc caataggaat aaaacaatat gagatcactc
      181 ccccgctatt tcctcgccct gcttgcaggc tctatcattt tcatcggtct tccattgctt
      241 ggttggggtc ttggaaacct accagctttc tttgagaacc cagcccgaac cgcgtatgtc
      301 gttgtcattt tcatcctgca aatcttttcc ctcatctaca atccgcaagt ggggcaaaat
      361 caggagaacc gaaagagtga tgcccctcgc aacaaactgg acttgatctt gattcagatc
      421 ttcagccttg gcattgtcat cctcgctcca ttttcagaca gccattcatt tggcgtactg
      481 aacattgaag tcattgttcg ttatctcggc ttgctgatcg ccatccctgg tttcatcctc
      541 atgcaagcag gtgaaaaaca tctcgccaga caattcagta ttgaagtcac tttgcaaaaa
      601 gatcacaaac ttattcaaag tggtccctat aaagtgatcc gccatccgcg gtatctggga
      661 atcctcatct tcttcactgg cattgcactt acttttcgtt ccctccttgg catttttacc
      721 gtcctcgctt tagcaggcgt tttgatctgg cgcgtcttta ccgaagaaaa gatgatgcac
      781 gaagaattcg gcaaagaatg ggaagaatat caaatcaaaa cctggagact tattccatac
      841 atcttctaat aaaaaatctc cgcataaagc ggaggttttt tattaccctt tgaagcgtgg
      901 atacgaaccc agcactcgca gcatcacagc atattcgccc aaatggtcaa gcgcgcgttg
      961 aagggtttca ttttctgtag agccaattag gtcaatgtaa aaaagatatt cccatggctt
     1021 gccttgtaat gggcgcgatt caattttggt caggtcaata tcccgcagcg caaaaacaga
     1081 catcgccttg aacaacgaac cggggacatt ttttagcgta aagacgatgg aagtcttcgc
     1141 atcacccgtc gggatagttg cttctcgttg gacagcgaga aaacgcgtaa agttttcctg
     1201 gttgtcttca atgccttctt ccagaatttg catgccatac aattccgctg cacgtttcga
     1261 ggcaattgca gccacatctt tcgcgccaga ctctttgagc attttcacac tgcccgccgt
     1321 gtcgtagacc tgctctggct tgatgcccaa attacgcaaa tacgccgcgc actgacccaa
     1381 cgcctgcgga tggctaatgg cttttttgat atcttctttt ttcacgccgg ggtttgcaat
     1441 caggcaatgc cgcacgcgca agaaatactc ccccacaata tgcaggttat gtcttaagag
     1501 cagatcataa ttctgatgaa tactgcccgc caatgagttc tcaatcggag ccagtgccgc
     1561 atcactcttg ccagaaatga cagagtcgaa catcacatca aatgattcac atggaaccgt
     1621 ctcaacatct ttgccgtaat aattgaaaac cgcttgctca ctgtacgcgc cgggttcgcc
     1681 ttgaaaggat attttcatgt tgtctccatt gattgggcgg gaagaccccg cccctactgt
     1741 ctttctgccc agttcacgat cttccgaaag cccgcttcca catcctcttc tgaagcggca
     1801 atcgtcatgc ggataaagcc ctcgccctgc tcgccaaacg cggagccggg cacaagccct
     1861 acgccttctt catctagaat cttttcgcac atttctacag atgacatctt taattcgcgc
     1921 aagtcaagga agaaatagaa cgcgccctgc ggtgggatga tcttcactct atcgctttcg
     1981 agttcgtgtg agagtcgaat gaccaactca cggcgacgtc cgtaggcagc gcgcatctcg
     2041 gcggtgacgt cttgaatctt cgggtcggtc aaggcgaagg cggcggcttt ttgaatgaag
     2101 ggagctacac aagtaatgga attctgcccg gctttcaagg cattttgaat cacaggtgca
     2161 ggtgccgcca aaaagcctac tcgccagccg gtcatagcat aagacttgga caagctattc
     2221 actaagatag tccgttcttt ggcgccttca atggctgcgg cagaagtggg tttgccttcg
     2281 taatataaac ttccgtagac ttcatcactg acaatccaca gattgtgttc ctttgcaaag
     2341 tcttgcagtt tttgtagata ctcacgcgaa gggaatgcac ccgtcgggtt ggaaggatag
     2401 ttgaggacga tgactcgcgt tttaggagtc aacgctttca gccaagaatc gaaatgaggg
     2461 atgaacccat tttcggcggg ggcagggatg cggatcacat ttccacgcag catgattgcc
     2521 atgttggaat gagtcgccca ggatggatcc ggaatcagca catcatcacc gggattcaaa
     2581 atggattgca tggcggtgta ataggcatgg atgccgccgt gtgtgacgag aatttccgag
     2641 gcagggtcgt agctcacgcc ttcatcgcgt tgaagtttag cagcaatggc cttgcgaagt
     2701 tcgagtgtgc cagcagaagg gccataatgt gtatgccctt cctgcatggc tttgaaggca
     2761 gcttctgcaa tggcggggtg tgtgccaaaa tcggggtcgc ccacttgcag gccgacgatc
     2821 ttgtgtccgc tggctttgag ggccagaata cggtcagcca tggcaacagt ggccgattga
     2881 cggatgaggt cgaattgtgt gttgaggtta tgcatggggt gagtgtatca aagaaccgaa
     2941 agtcccgtca acaacgacgg gactttcggt aacttgacgc atcaagggtt gagcagatgt
     3001 atatatagac tgtcagtc
//
LOCUS       DAOQJE010000420         3016 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_21937_length_3016_cov_5.840257, whole genome shotgun sequence.
ACCESSION   DAOQJE010000420 DAOQJE010000000
VERSION     DAOQJE010000420.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3016)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3016)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3016
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21937_length_3016_cov_5.840257"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..530
                     /locus_tag="PLT08_11135"
     CDS             <1..530
                     /locus_tag="PLT08_11135"
                     /inference="COORDINATES: protein motif:HMM:NF013378.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="YigZ family protein"
                     /protein_id="HNE05064.1"
                     /translation="FMKRIREEFADASHNVPVYMIGGGNTVTEYFSDDGEPSGTSGKP
                     ALTVLRGSGLGDAVLVVTRYFGGTLLGTGGLVKAYTEAAQSVVNVVERGRRVPVHVAL
                     AAIPYNLLERVRLLTTKNGGSILGEEYAADITLTLQFPVEYYTGFQTDLRELTAGKIQ
                     AEVIETKEEIIRVDK"
     gene            592..2790
                     /locus_tag="PLT08_11140"
     CDS             592..2790
                     /locus_tag="PLT08_11140"
                     /inference="COORDINATES: protein motif:HMM:NF024583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HNE05065.1"
                     /translation="MKDKNITIPTIETNRELQGEFFRAVILSIMALTGVFLVFAILIF
                     HELLLRTLIISGGVFFLSVFILVLIKRKKVFLASVFFVGFLWLLVASLTYLTGGVTAP
                     IFAGFLIVILFSGLIFNFRIGVSVALWTVLFGSVLIFAHSKGFLPLAVDYSNGARLAI
                     YTFFFFIALYIQKAAIDSTKLATEKAYISENQYQLFLENIPTITYINDISPEARTLYV
                     SPQVENLLGYSPKTFLQDPALWMKIINEDDVENVLLKNQASVETGEPFKVEYRLMTKS
                     GNVVWVRDEAKLVHDDHGEPAYWLGVWTDITKEKSSEKTLAYMVQTQSMRNNQLQTAS
                     EVSRAATSILELSELMPKVVELIRSHFDYYYVGIFLVEKVTNNAILAAATGSAGKEML
                     AKGHSLPIANSSMIGWSILNNRARIALDVGKDAVRFKNPLLPLTRSEVAFPLRFHGRV
                     IGAMTIQSALPSAFTDADITALQTMADQVANAIETARLLDEREALIDELETKNAELER
                     YTYTVSHDLKSPLVTIRGYVGYLKESAVKGDMQRFEVDMKRVIKATETMQALLNDLLE
                     LSRVGRVINSLTNVPFEDMVKDVLTLVVEPQKGQTEWFVVQKGLPTVRCDRTRILEVL
                     QNLILNSIKFIGDQPKPSIRIGQAGFDNKTNFPIFYVADNGIGIEPKYHETVFGLFNR
                     LDQKEEGTGIGLTLVKRIIEVHGGRIWLESEGRGKGTTFYFTLPPAEMKP"
BASE COUNT          832 a          558 c          709 g          917 t
ORIGIN      
        1 ccttcatgaa gcggattcga gaggagttcg cagacgcgtc tcataacgtc cctgtttata
       61 tgattggtgg tggaaatacg gtaacggaat atttttcaga tgatggagag ccaagtggta
      121 cctctggcaa acctgcgttg actgttttgc gtggcagcgg tttaggagat gcggttttag
      181 tagtcacccg ttattttggg ggcaccctgc ttggaactgg cgggctggtc aaggcgtata
      241 cagaggcggc gcagtctgtt gtcaatgttg tagaacgcgg aaggcgagtc cctgtgcatg
      301 tggcgctggc agcgattccc tataatttgt tggaaagagt tagattattg accaccaaga
      361 atggcggtag catcttaggc gaagagtatg ccgcagatat cacattgact ttacagttcc
      421 ccgtagaata ttacactggc tttcaaacag atctgcgaga acttacggct gggaaaattc
      481 aagcagaagt cattgaaaca aaagaggaaa tcatacgtgt agacaagtaa gtttttgagt
      541 tttactccct caataaaaaa acatgtataa agtaaaattc aaagtattgt tatgaaagac
      601 aaaaatatta ctattccgac cattgagact aatagggaac tgcaagggga gttttttcgt
      661 gccgttattt tatctataat ggctttgacg ggtgtttttc tggtttttgc cattcttatt
      721 tttcatgaat tgttattgcg gacacttatt attagtggtg gggtattttt tctttctgtt
      781 tttattctcg tcttgattaa aagaaaaaaa gtatttcttg cgagtgtttt ttttgtaggg
      841 tttctttggc ttttggttgc atctctgacc tatcttactg gtggagttac tgctcctatt
      901 tttgctggtt ttttaatcgt tattcttttt agcgggttga tttttaactt ccgtataggg
      961 gtttccgttg ctttatggac tgttcttttt ggtagtgttc tgatttttgc acactcaaaa
     1021 gggtttttac cacttgccgt ggactattca aatggcgctc gattggcgat ttatacattt
     1081 ttctttttta ttgcgcttta tattcaaaag gcagcaattg acagcacaaa gctagcaaca
     1141 gaaaaagctt atattagcga aaatcaatat cagctctttc ttgaaaatat ccccacgatc
     1201 acatacataa atgatatatc gccagaagcg cggacactat atgtcagccc gcaggtcgaa
     1261 aacttgcttg ggtattctcc aaaaaccttt cttcaagatc cagcactttg gatgaaaatc
     1321 attaatgaag atgatgttga aaatgttctt ttgaagaatc aagcttctgt tgaaacaggt
     1381 gagccgttta aagtggaata tcgtcttatg acaaaaagcg ggaatgtggt ttgggtgcga
     1441 gatgaagcca aactggttca tgatgaccat ggagaaccag cctattggtt gggggtgtgg
     1501 accgatatta ccaaggaaaa aagttcagag aaaacattgg cgtatatggt tcaaacgcaa
     1561 tccatgcgga ataaccagtt gcagacggcg agtgaagtgt cacgagcggc tacctctatt
     1621 ttagagttga gcgaattgat gcccaaagtt gtggagctaa tccgctcgca ttttgattac
     1681 tactatgtgg gtatcttttt agttgaaaaa gtgacgaaca atgccatact tgctgcggca
     1741 acaggttcag cgggaaaaga aatgcttgcg aaaggacatt ctttgcccat cgcgaactca
     1801 tccatgattg gttggtcgat tctcaataat cgggcgcgga tcgctttgga tgttggtaaa
     1861 gatgcggttc gtttcaaaaa tccgcttttg ccgcttacac gttcagaggt ggctttccca
     1921 ttgcgatttc atggaagagt tattggagcg atgacgattc aatctgcatt gccatctgcg
     1981 tttacagatg ccgatatcac cgctctgcaa accatggcag atcaggtagc aaatgcaatt
     2041 gagactgcac gtttattaga cgagcgcgaa gctctcattg atgagttgga aacaaaaaat
     2101 gcggaactgg aacgatacac ttataccgta tcgcatgatt tgaaatctcc acttgtaacg
     2161 atccgcggct atgtggggta tttaaaagag tctgctgtta agggagatat gcagcgtttt
     2221 gaagtggata tgaagcgcgt catcaaagcg accgagacaa tgcaagcact gctcaacgat
     2281 ttattggaac tttccagggt tgggcgtgta attaattctc taacgaatgt cccatttgaa
     2341 gatatggtga aggatgtttt gaccctggtc gttgagccgc aaaagggaca aacagagtgg
     2401 tttgttgtgc aaaaagggct tcctacagtg cgctgtgacc ggaccagaat tttagaagtg
     2461 ttacaaaacc tgatcttgaa ttcaataaaa tttatcggcg atcaacctaa accctccatt
     2521 cgcattgggc aagcgggctt tgataataaa acgaactttc ctatttttta tgtcgctgat
     2581 aatggaattg gcatcgaacc caaataccat gaaacggtct tcgggttgtt caatcgactg
     2641 gaccaaaaag aagaggggac agggattggg cttactttag taaagcgcat tattgaggtc
     2701 cacggcgggc ggatttggct tgaatcagaa gggcgtggga aaggtactac tttttacttt
     2761 accctgcctc ccgctgaaat gaaaccctag ttttacaact tcttcataat cttttcgcat
     2821 ttaatccacc tgaaataaag gcattttcat agaattaaca atagtccttt atatagagac
     2881 tgttaattga atattggaga gaaaatgtct aactcaaatc gaacaaaatt acttttggat
     2941 attggtacgt tcatcgcctt tctcattacc atggacccac gcttcagtgg actggcaatt
     3001 catgagtggc tatcac
//
LOCUS       DAOQJE010000421         3014 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_21962_length_3014_cov_5.554241, whole genome shotgun sequence.
ACCESSION   DAOQJE010000421 DAOQJE010000000
VERSION     DAOQJE010000421.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3014)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3014)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3014
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21962_length_3014_cov_5.554241"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..564
                     /locus_tag="PLT08_11145"
     CDS             <1..564
                     /locus_tag="PLT08_11145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE05066.1"
                     /translation="QPLPPTAISLPTQGPTNTAEPVNTATTAPTETLVPTSAPVATEV
                     VPTLSSSSTTELKPVNVSNDLGKGVFKDITFSSDLLTLQCYPREMTITITANLPEIVS
                     AIMYYRMVDNPDALYPSEWKNLGEMVSDKQGHFSFVLESLDLNPDLRVLEKAWIDFQF
                     IGINKGGGVVDRTQKIEKMVTYYKACP"
     gene            complement(606..863)
                     /locus_tag="PLT08_11150"
     CDS             complement(606..863)
                     /locus_tag="PLT08_11150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE05067.1"
                     /translation="MIRTIVFKTVASILLMLGALGLSMPMNAYAQSGTQHIQSAQYSG
                     KCEKIKDKKQKKACEKAQKAGKTKKNDDGPNHESNDGANHH"
     gene            complement(987..2303)
                     /locus_tag="PLT08_11155"
     CDS             complement(987..2303)
                     /locus_tag="PLT08_11155"
                     /inference="COORDINATES: protein motif:HMM:NF016408.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymer-forming cytoskeletal protein"
                     /protein_id="HNE05068.1"
                     /translation="MKTIQRFLAMFVLVALFSLTFASPALAFDGRSGDKIVIAADEVI
                     NDDLYVSAEEFTLDGTVKGDLVVFGAYIFINGTVEGDLIAAGNTIVIDGTVKDDARIA
                     GAALQLGEKASIGGDLISAGASLEAQNGSAVGQDVVFAGGQSLLAGSIERNLWVGTGA
                     LELSGEIGGDVQAEVGEMQEGSPSPSMYIGDVKVSTPSVQPGLTVTESAKIGGDFTYT
                     QSKDIKFPSDAIEGKVTRKEPTVDTQSVPTPPTAGEVAATWVFDLLRKIATLAIFGLL
                     LVWLVPGFLKGLGDKLQSQPIPSFGWGIVAYAAFFFAILLIVIVMIVGGIIFGVISLG
                     GVTGTIIWVGLLSIFAMIVGFALFTGFGAQILVAWLGGKWILGRFNPALAEHKVWPLL
                     LGVVIVGLLVKLPLVGWLIGFLIMFFGLGALWLWGRERMKKPEAVA"
     gene            complement(2435..>3014)
                     /locus_tag="PLT08_11160"
     CDS             complement(2435..>3014)
                     /locus_tag="PLT08_11160"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HNE05069.1"
                     /translation="PNGHTSAEKHPPRNDKGIMADELLDVVNDDDKVTGQEMRSVVHQ
                     QGLQHRGVHVFLFNEQGEMLIQKRSADRANSPSMWDCSVSEHVKAGESYLDAAMRGLK
                     EEMGVEGIEIALRGKIQMEYGFNDNEISEIYEGRLNGKSVQFDPVEISEVRFVSLDVV
                     KAGLSQSPELYCYWFIQLMNWYFGLPAKLKEL"
BASE COUNT          842 a          780 c          605 g          787 t
ORIGIN      
        1 caaccgcttc cgcctactgc aatttcactt cccactcaag gaccgacaaa cactgctgag
       61 cctgttaata ctgcgactac cgcccctact gagacattgg tacctacttc tgccccagta
      121 gcaactgagg ttgtaccgac tctttcgtct tcatcgacta cagaactgaa gccagttaat
      181 gtaagtaatg atttgggcaa gggcgtcttc aaagatatta ccttttcaag cgatcttttg
      241 acacttcagt gttatccgcg tgaaatgacc atcactatta ccgcaaatct tccagagatt
      301 gtaagtgcaa ttatgtatta tcgtatggtc gataaccctg atgcgcttta cccctctgaa
      361 tggaagaacc ttggcgagat ggttagcgac aagcaaggac atttttcgtt tgtcttggaa
      421 tcgctagatt tgaacccgga tttacgcgtt cttgaaaaag cttggattga tttccaattt
      481 attggtatca ataaaggtgg tggcgttgta gaccgtaccc agaagatcga aaagatggtg
      541 acttactaca aagcatgtcc gtaatagata ataaaaaagc gaccccactt ggggtcgctt
      601 ttttattagt ggtgattggc tccatcattt gattcatgat ttggtccatc gtcgtttttc
      661 ttagttttgc ctgctttttg ggctttctca cacgcctttt tctgtttctt gtcttttatc
      721 ttttcacatt taccgctgta ttgagcagat tgaatatgtt gtgtgccaga ttgtgcataa
      781 gcgttcattg gcatgcttag acccaaagct ccaagcatga gaaggatgga tgcaacggtc
      841 ttaaaaacga tagttctgat catggtactc tcctatttac tggtgattta ataggatagt
      901 cggtagtatt tgataaaagt tatgggtgtg aattaaagca caagcggcga ggaacttcct
      961 cgccgcttgt atgtgcgatt tatttgtcaa gcaaccgctt caggcttttt cattcgctca
     1021 cgtccccaga gccagagtgc gcccaaaccg aagaacatga ttaagaatcc gatcaaccaa
     1081 ccgaccaaag gtaatttgac caataagcca acaatgacca cgccgagcaa aagaggccag
     1141 accttgtgtt cagccaatgc ggggttgaag cgaccaagaa tccatttgcc gcctaaccat
     1201 gcaacaagga tctgagcacc gaaaccggta aacaaagcga acccaacaat cattgcgaaa
     1261 atgctcagca aaccaaccca gatgatggta ccagttacac caccaagaga gatcacaccg
     1321 aagatgatgc cgcctacaat cattacgatt acgatcaata ggattgcgaa gaagaaagca
     1381 gcataggcaa cgatgcccca gccaaagctc ggaataggct gactctgaag cttatctcca
     1441 agacctttca agaagcctgg cacaagccag accaacaaaa gcccaaagat ggcaagcgtt
     1501 gcaatcttgc gcagcaagtc aaatacccat gtagctgcaa cttcacctgc tgttggcgga
     1561 gtgggaacac tttgcgtatc aacagtcggt tctttgcgag tgacttttcc ttcaatagca
     1621 tcagaaggga acttaatgtc cttcgattgg gtgtatgtaa aatcaccacc aatttttgca
     1681 ctttcagtaa cggtcaaacc gggctgaacg ctaggcgtag aaacctttac atccccaata
     1741 tacatggaag gagacgggct gccttcctgc atttccccaa cttcagcttg cacatccccg
     1801 ccgatctcac cactcaactc caaggcgcca gtacccaccc agagattacg ttcgatcgaa
     1861 ccagcgagca aactttgtcc gccagcaaaa acaacatcct gccccacagc actgccattt
     1921 tgggcttcaa ggcttgcacc agctgaaatt aaatctccgc cgatagacgc cttctcaccc
     1981 agctgtaaag ccgcaccggc aatacgggca tcatctttta cagtgccatc gatcacgatc
     2041 gtatttcctg cggcgatcaa atcaccttcc acggtaccat tgatgaagat ataagcacca
     2101 aacaccacca aatccccttt gacggttccg tctaaagtga actcttctgc gctaacatat
     2161 aagtcatcat taatgacttc atctgcggca ataacgattt tatcgcccga acgcccatca
     2221 aatgccaagg caggcgaggc aaatgtcaga gagaacaagg caactaggac aaacattgcc
     2281 aaaaatcttt gaattgtttt cataaatact cctttgatag aaacttcctg aggaggatat
     2341 acgcccgaat atcccaaagg ttgcagagat gcgccctcgc ctctttctca gctcgtagtt
     2401 gacataacct attgaccttt aatgactttg aagcctatag ttccttcaat tttgcgggca
     2461 gaccaaaata ccaattcatt aattggataa accaataaca ataaagctca ggagattggg
     2521 aaagcccagc cttgaccaca tccaaactca cgaatcgaac ttcactaatc tctaccgggt
     2581 caaactgcac ggattttcca ttgagcctgc cctcgtaaat ctcactgatc tcattatcat
     2641 tgaaaccata ttccatttgg atcttcccac gcaaggcgat ctcaatgcct tccacgccca
     2701 tctcttcctt taaaccgcgc attgccgcat cgagataact ttccccagcc ttcacatgct
     2761 ccgacaccga gcaatcccac attgatggtg agttggcacg gtctgcactg cgtttttgga
     2821 tgagcatttc accctgctca ttaaatagaa agacatgcac accacggtgc tgtaaacctt
     2881 gttgatggac aaccgaacgc atttcttgtc cggtcacttt atcatcgtca ttgactacat
     2941 ccaataattc gtctgccata attcctttgt cattgcgagg cggatgcttt tccgccgacg
     3001 tgtgcccgtt aggg
//
LOCUS       DAOQJE010000422         3013 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_21975_length_3013_cov_4.451995, whole genome shotgun sequence.
ACCESSION   DAOQJE010000422 DAOQJE010000000
VERSION     DAOQJE010000422.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3013)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3013)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3013
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21975_length_3013_cov_4.451995"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            24..905
                     /locus_tag="PLT08_11165"
     CDS             24..905
                     /locus_tag="PLT08_11165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE05070.1"
                     /translation="MKVYDRTPLQDAQGNINPIARVQGTLKYGLSWAGELEAQKVVIA
                     QLDRLLDKGFVLIRNFTLPDSDIVIPMILIGPGSISVILATPLKGDFEAKGNEWNSVK
                     NGVSVPASRNLIDLLIKYTRVFQRYLQIHKINIPVQVEPVLIATNPGANIESVRPAVR
                     VVRSDAIKQFATSINQASPVLRAEQVIASAALIIEPHPASTEKPAEPQIPVERPMSRA
                     QAIFKASEEGDAEKKPKVAAPQPRPRPAQNAQGQAKPPAKKAPAISRRQIFLLVGLGI
                     FECCILFGGIYVLFFLS"
     gene            913..1644
                     /locus_tag="PLT08_11170"
     CDS             913..1644
                     /locus_tag="PLT08_11170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HNE05071.1"
                     /translation="MNTPFLSIIIPAHNEANRLPRTLEQVFAYLKLQNYSSEVVVVEN
                     GSSDNTLELAAHFIKEHSNLVVVQEHQKGKGNAVRRGMLVARGEYRFICDADLSMPIE
                     DLGKFLPPHLNDFDIAIGSREAPGAIRYNEPPYRHIGGRAINLAIRFLILPGLRDTQC
                     GFKCFRAETTTRLFEQQTLMGWSFDIELLYLARRKKLRIREIPIHWYFDAGSKVSAVR
                     DALRMISDIFRIHLNALRGTYDSKA"
     gene            1628..2257
                     /locus_tag="PLT08_11175"
     CDS             1628..2257
                     /locus_tag="PLT08_11175"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: protein motif:HMM:NF000595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HNE05072.1"
                     /translation="MIPKPDLTYENEIWSAYQFIAGMDEAGRGALAGPVSVGAVILPL
                     NHSHLLRALSGVHDSKQLTPRKRSELVPQIQATALAHGVGFASAEEIDQVGIVPATRL
                     AASRALEALSLYPQYLLTDFRLELPELDIPQTAIVKGDQRSLSIACASILAKTARDEF
                     MQKLNEKYPEYHLDHHKGYGTLFHRKKIETLGHSPIHRKTFQLKNNREA"
     gene            complement(2274..>3013)
                     /locus_tag="PLT08_11180"
     CDS             complement(2274..>3013)
                     /locus_tag="PLT08_11180"
                     /inference="COORDINATES: protein motif:HMM:NF013630.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HNE05073.1"
                     /translation="PPTPTPGTDGRIIYVVQAGDNCFRVAAINGITVEQLRQLNSKLD
                     ENCTLTEGQELLIGVAAVATATAPANVAVGTPTITPTPLSGLTEVCVLLFNDLNGDAM
                     RQDTEPAIAGGAVSVTEANGKYSASLETVIPSDPTVYQGTCFSNVPEGTYNITMAIPD
                     QYNPTMSLSYSLEVNAGDRAMVDFSAQSKDVVVDQTETEGNAGGASTGLVIFGIVLLL
                     GGAGLGYYAWRSNKPESKLAGGGILKK"
BASE COUNT          807 a          752 c          665 g          789 t
ORIGIN      
        1 ttccactctt ttaggcagat cgaatgaagg tatacgacag aactccattg caagacgcac
       61 aagggaatat caatcccatt gctcgtgttc agggtacgtt gaaatacggc ttgagctggg
      121 ctggggaatt ggaagcccaa aaagtggtga ttgcccaatt agaccgattg ttggataagg
      181 ggttcgtgtt aattcgcaac tttactctgc cagacagcga tattgtgatt ccgatgattt
      241 tgattggtcc ggggtccatt tcggtaatct tggcaactcc gctcaaaggg gattttgaag
      301 caaaaggcaa tgaatggaat agtgttaaga atggggtatc tgttccggca agccgaaatt
      361 tgatcgatct cttaataaaa tatacgcgtg ttttccagcg ttatcttcaa atccataaaa
      421 tcaatattcc agtgcaggtg gaaccagttt tgatcgcgac caaccccggc gccaatatcg
      481 aatcagtgcg ccctgctgtt cgtgtagtac gtagtgatgc gatcaaacaa tttgctactt
      541 caatcaatca ggcaagtccg gtcttacgtg cagagcaggt tattgcatcc gcagccctta
      601 tcattgagcc gcatcctgct agcacagaaa agcctgccga accacaaatt ccggttgagc
      661 gccccatgtc tcgtgcgcaa gcgatattta aggcttctga agaaggtgat gctgagaaaa
      721 agccaaaagt tgccgcccca cagccacgtc cacgcccggc gcaaaatgct caagggcagg
      781 caaaacctcc tgccaagaaa gcacctgcaa tctctcgtag acaaatattc ctattagtag
      841 ggttgggcat ctttgagtgc tgtattctat ttggtggcat ctacgtcctg tttttcttaa
      901 gctaacatcg ctttgaacac gccattctta tctatcatca tccctgccca taatgaagca
      961 aatcgcctgc cgcgcactct cgagcaggtt tttgcatatc taaagttgca aaactactca
     1021 agcgaagtag tagttgtgga aaatggaagt tcagataaca cgcttgagct tgcggctcac
     1081 tttataaaag aacattctaa tttggttgtt gtccaagagc atcaaaaagg caaagggaat
     1141 gctgttcggc gcggaatgct tgtagcacgt ggtgaatatc gcttcatttg tgatgctgat
     1201 ctttctatgc caattgaaga tttagggaaa ttcctaccac cccatctcaa tgactttgat
     1261 attgcgattg gttcacgtga agcccccggt gccatacgtt ataacgagcc accctatcgc
     1321 catattggcg gacgtgccat taaccttgca atccgttttc tgatcctccc aggtctgcgt
     1381 gacacacaat gcggatttaa atgttttcga gcagaaacga caacccgatt gtttgaacaa
     1441 caaaccttaa tgggctggtc ttttgatatt gagttactct acctggctcg tcgaaaaaaa
     1501 ttacgcattc gtgaaatccc tattcattgg tattttgacg caggcagtaa agtaagtgca
     1561 gttcgcgatg ctctacgtat gatcagcgat atcttccgta ttcacctgaa tgcccttcgt
     1621 ggcacgtatg attccaaagc ctgatttaac ctacgaaaat gagatttggt ccgcgtatca
     1681 attcatagca ggcatggatg aagccggacg cggcgcactg gctggaccgg tctcagttgg
     1741 ggcagtgatt ctccctctca atcattcgca tcttctccgc gccttgagcg gagttcacga
     1801 ctctaagcaa ctcacacccc gaaaacgctc cgaactcgtt ccacaaattc aagcaactgc
     1861 cctcgcccac ggagtcggat tcgcctccgc tgaagaaata gatcaagtgg ggattgtgcc
     1921 agctacacgt ctagctgcga gccgtgcatt ggaagcttta tctctttatc cgcagtactt
     1981 actaacggat ttccgtcttg agctccctga gttagatatt ccgcaaactg ccattgtcaa
     2041 aggagatcaa cgctcattaa gtattgcctg cgcatccatt ctggcgaaaa ctgcccgcga
     2101 tgagttcatg caaaagctga atgagaaata cccagaatat cacttggacc atcacaaagg
     2161 ctatgggacg ctctttcatc gaaagaagat cgaaacgctc gggcactccc ccattcaccg
     2221 aaaaactttt cagttaaaaa acaaccgcga ggcttaagcc tcgcggtctt catttatttt
     2281 ttcaagatgc cgccaccagc taatttactt tctggcttat tcgagcgcca ggcataatac
     2341 ccaagcccag ccccgccaag taataaaaca ataccgaaga tgacaagccc agtggatgca
     2401 ccacctgcat ttccttctgt ctcagtctgg tctaccacta catctttcga ttgagcgctg
     2461 aaatctacca tagcacgatc accagcattc acttccagag aatagctcaa cgacatcgtt
     2521 gggttatatt ggtcagggat agccatggtg atgttatatg tcccttcagg aacatttgaa
     2581 aaacaagtac cctgatatac cgttgggtct gatggaatga ctgtttctaa tgaagcagaa
     2641 tattttccat tggcttcggt cacacttact gcgccaccag caatagcggg ttctgtatct
     2701 tggcgcatag cgtcaccgtt aagatcattg aacaaaagca cacacacttc tgttaaacca
     2761 gaaagcggag ttggagtaat ggttggcgtc cccactgcta cattcgcagg cgctgttgcc
     2821 gtcgccacag ccgcaacacc gatcaataat tcctgtccct cagtcaacgt acaattttca
     2881 tccaactttg aattcaactg acgaagttgt tccaccgtga tgccattgat cgcagccacg
     2941 cggaaacaat tatcgcctgc ttgaacaaca taaataatac gcccatccgt gccaggggtt
     3001 ggtgtaggag gag
//
LOCUS       DAOQJE010000423         3005 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_22062_length_3005_cov_5.463390, whole genome shotgun sequence.
ACCESSION   DAOQJE010000423 DAOQJE010000000
VERSION     DAOQJE010000423.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3005)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3005)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3005
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22062_length_3005_cov_5.463390"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..771)
                     /locus_tag="PLT08_11185"
     CDS             complement(<1..771)
                     /locus_tag="PLT08_11185"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNE05074.1"
                     /translation="MKHLVKNNRICAVCGGSARELLFQQKFTEISDSSILAGYDIVVC
                     QTCGFCFADNLPDQSAFDVYYREMSKYEHQDRSGQASEFELRQFPALAQFIQRHISER
                     EARILEIGCANGGLLNALKNLGYKNLLGVDPSPVCARNAEQLYQIKVLTCALSDLKVE
                     TGPFNFIILVAVLEHIKDLESALNKLSNLLSPVGKIYIEVPDATQFTSSPDAPFQEFS
                     IEHINFFSPTSLANLMGAYGFSEVFTTQVSYDQTDTHTG"
     gene            complement(805..2010)
                     /locus_tag="PLT08_11190"
     CDS             complement(805..2010)
                     /locus_tag="PLT08_11190"
                     /inference="COORDINATES: protein motif:HMM:NF016908.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FkbM family methyltransferase"
                     /protein_id="HNE05075.1"
                     /translation="MSTKIAGEKSTQDLEKLLSQPLEKIQRWESNRYDELAGAFSKSL
                     VLYGAGGLGRKTLKGLKTLGITPLAFTDNNPNLHGRLIEGVTVMSAQEAAKEFGESAV
                     FLVTVYTDSAPGEIDLIIKKLSDLGCKKVIPFGPLFWKHPEQFLPHYAYDLPHKVIEA
                     ADSILKTWALLEDDISQNEYLAQIQWRLNPEYSQIPAQANHDIYFPPELITLIENEVF
                     IDCGAYNGDTVRSFLKQSNGKFDKLIAFEPDPVNYEKLVELAALLPQDTARRIQTSDF
                     ALGSRSEKLYFDALGVASSSLSINGSIQIQSEPLDKLLVNEMPTYIKMDIEGAEVDAL
                     SGASQSIRKFLPILAVSVYHHQDHIWTIPLMIHALSKEYKFYLRRYTPHVLDDLVLYA
                     IPKSREPAR"
     gene            complement(2000..2986)
                     /locus_tag="PLT08_11195"
     CDS             complement(2000..2986)
                     /locus_tag="PLT08_11195"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HNE05076.1"
                     /translation="MSNWNKKRVLVTGAGGFIGSHLTERLVEEGAEVRALVHYNALGR
                     WGWLDRSPHLKEMEVISGDITDRDFVRQAVKSREIVFHLGALIAIPYSYQVPLSYVRT
                     NTEGTINILQSARDEGLERVVHTSTSEVYGTAQYVPIDEKHPLKGQSPYAASKIGADQ
                     MAEAFHLSFNVPVVTVRPFNTFGPRQSTRAIIPTIIVQCLLGDTIRLGNLHPTRDLNY
                     VSNTVDGFLAAASEPSALGQTFNLGSNREISIGDLADLIAKMVGKPIKIESEDKRLRP
                     SGSEVERLWADSTLAQKVLGWQPEIKLEEGLERTIAWLKDNLDLYRGNAYVY"
BASE COUNT          750 a          675 c          725 g          855 t
ORIGIN      
        1 gccagtgtgg gtatctgtct gatcgtagga aacctgggtg gtaaatactt cggaaaatcc
       61 atacgcaccc atcaggttgg caagcgatgt aggtgaaaaa aaattgatat gctcgatgct
      121 gaattcctgg aaaggtgcat caggtgagga tgtgaattgt gtcgcatcgg gcacttctat
      181 gtagatcttt ccgacgggag agaggagatt tgatagcttg tttaaggcgc tttccagatc
      241 tttgatatgt tccaaaacgg ccacaagaat gatgaaattg aacgggccag tctcaacctt
      301 caggtcggat agagcgcagg tcagcacttt gatttgatac agttgttctg cgtttcgcgc
      361 gcagactgga gaagggtcaa cgccaaggag gtttttatac ccgagattct ttaaagcatt
      421 taatagacca ccgttggcgc agccgatttc cagaatacgg gcttcccgct ctgagatgtg
      481 cctttgaatg aattgagcta gtgcggggaa ctggcgtaat tcaaattccg atgcctgtcc
      541 tgagcgatcc tggtgctcgt atttcgacat ttcacgataa tacacatcga aagccgattg
      601 gtcgggcaga ttatcggcga agcaaaatcc acaggtttga catacaacaa tatcatagcc
      661 agccagaatg cttgagtcac taatctctgt aaatttttgc tggaacaata attcacgggc
      721 acttccgccg caaactgcac aaatacgatt gttttttact agatgtttca tggttttctc
      781 cgcaggttga cctaattctg gaaattaacg ggcaggttca cggctttttg gaatggcata
      841 gagcacaagg tcgtccaaga catggggcgt ataacgtcgt aaataaaatt tgtattcttt
      901 cgataacgca tggatcatga gcggaatggt ccagatgtgg tcttggtggt ggtagaccga
      961 cactgccaga atgggcagga atttgcgaat ggattgactt gcgcctgaga gagcatcaac
     1021 ttctgcgcct tcgatatcca ttttgatata ggttggcatt tcgttgacaa gtagtttgtc
     1081 gagtggctca ctttggattt gaatactgcc attgatgcta agtgaggaag aagcgacacc
     1141 tagagcatcg aagtataact tttcggaacg actacctagt gcgaagtcgc ttgtttggat
     1201 gcgccgggct gtgtcttgtg gaagcaaggc tgccaattct actaattttt catagttgac
     1261 cggatcaggt tcgaatgcaa ttaatttgtc aaattttccg tttgattgct ttagaaaact
     1321 gcggaccgta tcaccattgt aggcaccaca gtcaatgaat acttcgtttt cgattagtgt
     1381 aatcagttct ggggggaaat aaatatcgtg atttgcctgt gcgggaatct gactgtattc
     1441 aggattaagt cgccattgga tttgcgcaag atattcattt tgcgaaatat catcttccag
     1501 caatgcccat gttttcaaaa tggaatccgc tgcttcaatc accttatgag gcagatcgta
     1561 tgcataatgg ggtaggaatt gctctgggtg tttccagaat aagggtccaa atggaataac
     1621 ctttttgcat cctaaatctg ataacttctt tatgatcaga tcaatttcac cgggcgccga
     1681 atctgtataa acagtaacca gaaagaccgc gctttcaccg aattcctttg ccgcttcctg
     1741 ggcagacata acagtcacac cttcgatcaa tcgtccatgc agattggggt tgttgtcggt
     1801 gaatgccagc ggcgtgatgc caagggtttt taatcctttg agtgttttac ggcccaaacc
     1861 accagcacca tacaagacca gcgattttga aaatgctcct gccaactcat catagcggtt
     1921 cgattcccac cgttggattt tttccaatgg ctgcgaaagt aatttttcaa gatcctgggt
     1981 tgatttttcg cccgcaattt tagtagacat aggcattccc acgatacaag tcgagattgt
     2041 ccttgagcca ggcaattgta cgctcaagac cttcttccag tttaatttca ggctgccatc
     2101 ctaatacttt ttgtgcaagg gtgctgtctg cccacaatcg ctccacttca ctgcctgatg
     2161 ggcgtagacg tttgtcttcg ctttcgatct tgatcggttt accgaccatt ttcgcgatca
     2221 ggtccgcaag gtcgccgatg ctgatttcgc ggttcgagcc gagattgaag gtctgtccca
     2281 atgcagaggg ttcggatgct gccgcaagga atccatcgac agtgtttgat acataattca
     2341 aatcgcgggt tgggtgaaga tttccaaggc gaattgtatc tcccaagagg cattgaacaa
     2401 tgatggttgg aatgatggcc cgggtggatt gacgcggtcc aaacgtattg aatggacgaa
     2461 ccgtcaccac aggcacgtta aacgagagat gaaaagcctc tgccatttga tctgcaccga
     2521 tcttgctggc cgcatatggt gactgtccct tcaatggatg tttttcgtca atggggacat
     2581 attgggctgt accatatacc tcactggtcg aggtgtgaac cacacgctcc aagccttcat
     2641 ctcttgcgga ttgcagtata ttgatcgtgc cctccgtatt cgtgcggaca taggacaaag
     2701 gaacttgata tgaatatggt attgcaatca gtgccccgag atgaaaaacg atctccctgc
     2761 ttttcacggc ctgccttaca aagtcacggt cggtgatatc tcccgagatc acttccattt
     2821 cttttaggtg tggagaacga tccagccaac cccatctgcc caatgcgttg taatgcacca
     2881 gagcacggac ctctgctcct tcttccacaa ggcgttctgt tagatgactg ccgatgaacc
     2941 ctcccgctcc tgtgactaat acacgctttt tgttccagtt tgacatcgct tcgactccat
     3001 tttct
//
LOCUS       DAOQJE010000424         3001 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_22109_length_3001_cov_5.068907, whole genome shotgun sequence.
ACCESSION   DAOQJE010000424 DAOQJE010000000
VERSION     DAOQJE010000424.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3001)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3001)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3001
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22109_length_3001_cov_5.068907"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            173..631
                     /locus_tag="PLT08_11200"
     CDS             173..631
                     /locus_tag="PLT08_11200"
                     /inference="COORDINATES: protein motif:HMM:NF016184.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein tyrosine phosphatase family protein"
                     /protein_id="HNE05077.1"
                     /translation="MKDIYNFLYYNDKLSSSGMPTETQVKSIAEAGVQLVINLAPHDV
                     PHAIPEEEKVVHSFGMEYINIPVNWGTPTRDGLHVFMDNMDANAGKKIHVHCEANFRA
                     SAFVAIYRILRLGWKEEEAFGIMHKIWDEDAYPVWKMFIENAIQRSADGS"
     gene            680..1174
                     /locus_tag="PLT08_11205"
     CDS             680..1174
                     /locus_tag="PLT08_11205"
                     /inference="COORDINATES: protein motif:HMM:NF023580.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3592 domain-containing protein"
                     /protein_id="HNE05078.1"
                     /translation="MIGKLDPEVLRANETKMNKRGKIIGPVMMVGSVLLSVILGIIAY
                     GSTIQRINWSQTSGTVTRSESSSDIIRNEEKYYYEYEYVVDGVTYTGKDSDTGRTVSN
                     PPSKGTSITVYYNPANPEKSLTDTNKSDTISDFAVWMFCCGPIFFLFGAVILFMSTRK
                     SQIA"
     gene            1178..1924
                     /locus_tag="PLT08_11210"
     CDS             1178..1924
                     /locus_tag="PLT08_11210"
                     /inference="COORDINATES: protein motif:HMM:TIGR02206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02206 family membrane protein"
                     /protein_id="HNE05079.1"
                     /translation="MNLQSFFSVNYTDTPLQIFGSKHLIVVAIFVLVWLSFVYFRKTW
                     NDKQKNTIRIALAIFLAVNEIGLHIWSAYWGIWNIQTMLPLHMCSVMVWVTTYTALTD
                     NRTLYDFTYFLGIGGALQAFLTPVDVAMYDLPHYRSLQSLIAHGLLITIPIFMTVVEG
                     YRPTFASFKRIFIWTNIYMVIIFFLNRVIGSNYLFIAQKPPSPTLMDMLSPWPWYIPQ
                     LEVVAFIIFFILYIPFLIKDQRAKQSVRQA"
     gene            1939..2862
                     /locus_tag="PLT08_11215"
     CDS             1939..2862
                     /locus_tag="PLT08_11215"
                     /inference="COORDINATES: protein motif:HMM:NF012958.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHB depolymerase family esterase"
                     /protein_id="HNE05080.1"
                     /translation="MKTKYFFFLISALIFVSMACRKTANTGVGIQNEYTLNYQNIERS
                     YILYTPKGLDTSKPMPLVFVLHGGGGNAENIIRSSGFNEVADQNGALIVYPNGTGRLN
                     DAILTWNGGTCCGFAQENNIDDVGFIREILKDVESIENVDPKRIYATGMSNGGIMSYR
                     LACEASDIFAAVGSVSGTLNFSPCMPTENVSIIHFHGTEDQHVPYDGGVGSQSLVGVD
                     FASVQDSVNFWVEFNGCNSQPQTNSFEDVQHQAWKGCAGFSSVELYTIVGGKHAWPGS
                     QGPGWVGGDEPDQTISATQLLWEFFASHPKP"
BASE COUNT          840 a          671 c          655 g          835 t
ORIGIN      
        1 ccattggtgg cgtgcctccc ttgggtcatg tagaaccaat ggaaacctat ctcgacgaag
       61 atttttcaaa atacgccaca gtatgggcag cagcgggaac acctaaagcc atctttgaac
      121 tgaacaccga agacctgcaa aaaatgactg gcgggaaagt ggtcaaagta gtatgaaaga
      181 catttataac tttctatact acaacgataa actctccagc agtggtatgc caacagagac
      241 tcaggtaaag tcgatagctg aggcgggtgt gcaattggtc attaatcttg ctccacacga
      301 cgttccacat gcaattccag aagaagagaa agtggttcat tcttttggca tggaatacat
      361 caacattccg gtcaattggg gtacaccaac acgtgatgga ctacatgtat tcatggacaa
      421 catggatgcc aacgctggca aaaagatcca tgtccattgt gaggcgaatt ttagagccag
      481 cgcatttgta gcaatctacc gcatcttgag attaggctgg aaggaagaag aggcgtttgg
      541 aattatgcat aagatttggg atgaagatgc atacccggtc tggaaaatgt tcattgagaa
      601 tgccatccaa cgaagcgcag acggttctta gcgggtagaa tattattaca tccaaaaatc
      661 caattgatag ggagaagcta tgatcggaaa actcgaccca gaagttttac gcgccaatga
      721 aacaaaaatg aataagcgcg gcaagattat cggaccagtg atgatggttg gctctgtact
      781 tttgagtgta atcttgggga tcattgccta tggcagcacg atacaaagaa tcaattggtc
      841 gcaaactagc gggacagtta ctcgatccga gtctagcagc gatattatta gaaatgaaga
      901 aaaatattat tacgaatatg aatatgtagt ggatggcgtc acttacactg ggaaagactc
      961 cgatacaggg cgtacagtca gcaacccgcc ctcaaaaggg acatcgatta cggtgtatta
     1021 caaccctgct aaccctgaaa aatctttaac cgataccaac aaaagcgaca ctatcagtga
     1081 ctttgccgta tggatgtttt gctgcggtcc cattttcttt ctatttggag cagttatcct
     1141 attcatgtct acacgcaaat cacaaattgc ataaactatg aacctacaaa gttttttctc
     1201 tgtcaactat acagatacac cgctccagat atttggctct aagcatttga tcgttgttgc
     1261 tatttttgtt ctggtctggc tttcctttgt ctacttccgc aagacttgga atgataaaca
     1321 gaaaaacacg atacgcatcg cactggctat ttttttagca gtcaatgaga ttggtcttca
     1381 catttggtcg gcgtattggg gcatttggaa tatccaaacc atgcttcctc tgcatatgtg
     1441 tagtgttatg gtttgggtca ctacctatac tgctctcaca gataaccgaa ctctctacga
     1501 cttcacttac tttttaggca ttggcggtgc attacaggct tttctcaccc cggtagatgt
     1561 cgccatgtat gacctgccac attatcgcag tctgcaatca ttgattgccc acggattact
     1621 aatcaccatc ccgattttta tgaccgtggt tgaaggctac cgtccaacat ttgcatcttt
     1681 caagcgcatc tttatctgga cgaatatcta catggtcatc attttcttcc taaatcgcgt
     1741 cattggcagc aattacctgt ttatcgctca aaagccgcct tcccccaccc tcatggacat
     1801 gctctctccc tggccctggt atattccaca acttgaggtg gtggcgttca ttattttctt
     1861 cattctgtac atcccgttcc tcattaaaga tcaaagagca aagcagtcag ttcgtcaggc
     1921 gtaatactag ttttgcccat gaaaaccaag tatttctttt ttctaatctc tgcactgata
     1981 tttgttagca tggcatgtag aaaaacggca aatacaggcg taggtattca gaatgaatac
     2041 actttaaact accaaaacat cgagcggagt tacatccttt atactcctaa agggctcgac
     2101 acatctaaac ctatgccgct ggtatttgta ttacacggcg gaggcgggaa tgcagaaaat
     2161 attatccgct ccagtggttt taatgaggtt gctgaccaga acggagcatt gatcgtttac
     2221 ccaaatggga cgggtcgact caatgatgca atccttacct ggaacggcgg cacttgttgc
     2281 ggctttgctc aggaaaacaa cattgatgat gttggtttca ttcgtgaaat cctgaaggat
     2341 gtggagtcca tcgaaaatgt tgacccgaaa cgaatctacg ccacagggat gtcgaacggg
     2401 ggcatcatgt catatcggct tgcatgtgaa gcttcagata tttttgcagc cgttgggtct
     2461 gtctctggca cgcttaattt ttctccttgc atgccaaccg aaaacgtatc gatcatccat
     2521 ttccacggta cagaagacca acatgttcct tatgacggcg gtgtagggtc tcaatcactt
     2581 gtcggtgtag attttgcctc ggttcaagac tctgtcaatt tttgggttga atttaatggc
     2641 tgtaactcac agccacaaac caattctttt gaggatgttc agcatcaggc gtggaaagga
     2701 tgcgcaggtt tttcctccgt ggagttatac actatcgttg gaggaaaaca cgcctggccc
     2761 ggcagccaag gtcccggttg ggtcggcggc gatgaaccgg atcaaaccat ttctgccact
     2821 caattgttgt gggaattttt cgcatctcac ccaaaacctt aatttatttt ttaattgcat
     2881 ccataggtct tttatcgtag aatcctaaag acattattca tcccatatcc atgggaaaac
     2941 caacctaaag gagagaaaat ggtagcgaaa gccaagaatg aatcacgccc catgaccgag
     3001 g
//
LOCUS       DAOQJE010000425         3000 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_22125_length_3000_cov_4.006112, whole genome shotgun sequence.
ACCESSION   DAOQJE010000425 DAOQJE010000000
VERSION     DAOQJE010000425.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3000)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3000)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3000
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22125_length_3000_cov_4.006112"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(211..1311)
                     /locus_tag="PLT08_11220"
     CDS             complement(211..1311)
                     /locus_tag="PLT08_11220"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013227.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HNE05081.1"
                     /translation="MEEIPAIDLKRQYQSIRKEIDEAYARVMSGGNFILGTEVAAFEK
                     EFAEYCGVSYAIGVASGTEALQLALMACGVEEKATVLTTAHTAVATVGAIEASGAYPK
                     LLDIDLSRYTLDPAELEKSINEQTHAIVPVHLYGCSANMRPILDVAKEKKLFVIEDAS
                     QAHGALYFGHRVGAMGHAAAFSFYPTKNLGAFGDGGAVLTNDSGIAERVRLLRQYGWK
                     EHYVSSVKGINSRLDELQAAILRVKLRHLDEWNQRRNQLADLYFELLADTDLVLPLRP
                     DDCEHVFHQFVIRHPRRDALREYLLSKGIHTLIHYPVPIHLQPAYKNLGLNPGDLPKA
                     EQAAREMLSLPIYPELTEREVRRVAEAVRSFF"
     gene            complement(1329..2321)
                     /locus_tag="PLT08_11225"
     CDS             complement(1329..2321)
                     /locus_tag="PLT08_11225"
                     /inference="COORDINATES: protein motif:HMM:NF027681.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HNE05082.1"
                     /translation="MIDVNSSYFQKNVLITGGAGFIGSNLASTLLGLGATVTIFDNFE
                     SDSGANRFNLEGIDNHIRLIQADIRDEVAMKDAVQGQDFLFNLAGQMSHIGSMQDPFK
                     DLYVNTVGQLNLLEVCRQHNPKIRVVYAGTRQVYGRPKYLPVDEKHLLTPLDNNGVSK
                     RAGEMYHIVYHHVHGMATCVLRMTNTYGERMRIKDDKLTFIGWWIQRILTGNEIQIFG
                     DGKQIRDFNHVSDVIRAMLLCAVNPAADGKIYNLGGEPISLIDLAALLVEVNGSGAFT
                     TVPFPENRKRIDIGGYYGDYSLIKNEIGWQPQVSLKDGLAQMLAFYREHKSYYL"
     gene            complement(2318..>3000)
                     /locus_tag="PLT08_11230"
     CDS             complement(2318..>3000)
                     /locus_tag="PLT08_11230"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HNE05083.1"
                     /translation="GGTIASMVAAASIACKQAANDFEIIVVNDGSSDYTASILEELTH
                     KYPQLRVLTHHSNQGYGATLKTGFTAAQKDWVFYTDGDSQYNPLELVNLVNALQEGVD
                     LVNGYKLTRNDSWIRIVIGRAYHYLVKFLFNIPIRDVDCDFRLIPRNVLDEIDLKSVS
                     GAICLEMVKKIADAGYVFAEVPVNHYSRKYGVSQFFVPWRVLRTLQQIAQLYWMLVIR
                     KEHLKNKK"
BASE COUNT          854 a          748 c          563 g          835 t
ORIGIN      
        1 caaacgatgg tactcaagag catcttcacg tgtgaattta gccatacagc ctccagaagt
       61 ttagtttgca actatcgata aaattatcgc acgactttac aggcgggaat agttgctttt
      121 gtcataattt ggggagttta gacagcccta ttatgtttta cccttattcg agaaaaagtt
      181 ccgcagaaag cctatcaatc cctcgcaggc ttagaaaaat gatcgaactg cttcagccac
      241 tcgccgtact tctcgctcgg tcagttcggg gtagatgggc aaagaaagca tttcacgtgc
      301 ggcttgttct gcctttggca aatcgccagg gttaagtccc agatttttat aagcaggctg
      361 aaggtgaatc ggaaccggat aatgaatcaa agtatgaatc ccctttgaaa gtaaatattc
      421 cctcaatgca tcgcggcgcg ggtggcgtat cacaaattga tggaagacgt gttcacaatc
      481 atccgggcga agcggcagaa caaggtctgt atctgccaat aattcaaaat acaaatctgc
      541 gagttgattt cgtcgttgat tccattcatc caaatggcgc aacttaactc ttaagattgc
      601 cgcttgtaat tcatccaagc ggctgttaat ccctttgacc gaacttacat aatgttcttt
      661 ccaaccatac tgacgcaaaa gccgcacccg ctcagcaatg ccagagtcat tggtgagcac
      721 agcgccccca tcgccaaagg ctccaagatt ttttgtggga taaaaactaa atgcagcagc
      781 atgtcccatc gcgcccactc tatgtccaaa atacaaagcg ccatgggctt gcgacgcatc
      841 ttcaataaca aataattttt tttctttggc aacatccaaa attggtcgca tattggcaga
      901 gcaaccatac aagtgcaccg gaacaatcgc atgtgtttgc tcgttgattg acttctccaa
      961 ctcagctgga tccagcgtat atctggataa atcaatatct aacaattttg gatatgctcc
     1021 gctcgcctct atcgcaccga cagttgccac tgctgtgtga gcagtcgtca acactgtggc
     1081 tttttcttct accccacaag ccatcaacgc caattgcaaa gcttctgtcc ccgaagcaac
     1141 accaatcgca tatgaaactc cacaatactc tgcaaattct ttttcaaatg ccgcgacttc
     1201 ggttcctaaa ataaagtttc caccagacat cactctggca taggcttcgt caatttcttt
     1261 acgaatcgat tgatattgtc gtttaaggtc aattgctgga atttcttcca ttacacacca
     1321 tagccattct acaaataata tgatttatgc tctcgataaa aagcaagcat ttgcgctaaa
     1381 ccgtctttga gtgagacttg tggctgccag ccaatttcgt tttttatcaa tgagtaatcg
     1441 ccatagtacc cgccaatatc aatacgtttg cgattctcag gaaaagggac agtagtgaat
     1501 gctccgctac cattgacttc aactagcaat gcagcaaggt cgatcaaact aattggttca
     1561 ccaccaaggt tatagatttt cccatccgcc gcaggattca cagcgcataa caacatggct
     1621 cgaattacgt cacttacatg gttgaaatct cgaatttgtt tgccatcgcc aaaaatctgg
     1681 atttcattcc ccgtcagaat acgttgaatc caccagccaa tgaaggtcag cttatcatcc
     1741 ttgatcctca tcctctcgcc ataggtattt gtcatacgca atacacaagt cgccatccca
     1801 tgcacatggt gataaacaat atgatacatc tcgcccgcac gcttgcttac tccgttgtta
     1861 tccaacgggg tcagtaagtg tttttcgtcc acgggtaaat attttgggcg accatatacc
     1921 tgacgggtcc cggcatatac aacgcgtatc ttcgggttgt gttgccggca aacttccaga
     1981 agatttagtt gccccactgt atttacatat aaatctttaa atggatcctg catgctgcca
     2041 atatggctca tctgcccggc aaggttaaaa agaaagtctt gcccttgaac agcatctttc
     2101 atcgctactt catcgcggat atccgcctga atgagccgaa tatgattgtc gataccttca
     2161 aggttaaagc gatttgctcc agaatcggac tcaaaattat caaagatcgt aacggtagca
     2221 cctaaaccta gcaatgtgct cgccaaatta ctgccaataa aacctgcgcc acctgtaatc
     2281 aacacatttt tttgaaaata gcttgaattt acatctatca ttttttattt ttcaagtgtt
     2341 cttttcgtat gacaagcatc caatacaatt gagcgatctg ctgaagtgtg cgtaaaacac
     2401 gccaaggcac aaaaaactgc gagaccccgt actttctcga ataatggtta accggaacct
     2461 cagcaaaaac ataccctgca tctgcaatct ttttgaccat ctccaagcag atggcaccac
     2521 tcacactttt taaatctatt tcgtcaagca cgtttcttgg gataaggcga aagtcacaat
     2581 ctacatcccg aataggaata ttgaaaagaa acttcactaa atagtgatat gcccgcccaa
     2641 tcacgatacg tatccacgag tcattgcgcg ttaatttata cccattaacc aaatccacac
     2701 cttcttgtaa agcattgacc aagttaacca attccagcgg gttatattgc gaatcaccat
     2761 ctgtataaaa aacccagtct ttttgggcgg cggtaaaccc agtttttagt gttgccccat
     2821 acccctgatt cgaatgatga gttagaacac gcaattgagg gtatttatga gtcaattcct
     2881 caagtatgct tgctgtatag tctgagctgc catcattgac tacaataatc tcaaaatcat
     2941 tcgccgcctg cttgcaagcg atactagctg ccgccaccat gcttgcgatt gttccgccat
//
LOCUS       DAOQJE010000426         2997 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_22167_length_2997_cov_5.386132, whole genome shotgun sequence.
ACCESSION   DAOQJE010000426 DAOQJE010000000
VERSION     DAOQJE010000426.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2997)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2997)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2997
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22167_length_2997_cov_5.386132"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(42..941)
                     /locus_tag="PLT08_11235"
     CDS             complement(42..941)
                     /locus_tag="PLT08_11235"
                     /inference="COORDINATES: protein motif:HMM:NF013998.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HNE05084.1"
                     /translation="MNTSTSARTPEHILHRLDWQVIRRLDGYLQGDYRSLFRGSGVDF
                     ADLREYQPQDDIRYIDWNVSARMDTPYVREYMEDREIIAWFLLDLSPSVDFGTLDTQK
                     RTVLVDFVGVLSRLLTRHGNRVGAVMYGSGVQHTVPARGGRMHVLRLINDLLKQPRLT
                     RVPMTDLSALIRGGLHSIKKRSLVFVVSDFISTPGWERSLSLLAQRHEVIAIRLWDPR
                     ETTLPDVGHIVLEDAETGEQLFVDTSDKKFRQRFMEAANKREAALADSFKRAGVDVLS
                     LSTEADLIHSIASFVMLRRQRRK"
     gene            complement(910..1917)
                     /locus_tag="PLT08_11240"
     CDS             complement(910..1917)
                     /locus_tag="PLT08_11240"
                     /inference="COORDINATES: protein motif:HMM:NF040105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HNE05085.1"
                     /translation="MEQTNRENQLPMERVLYEVKKVIVGQDHLLERLVVALLARGHIL
                     VEGVPGLAKTMAIKTLADSIGGEFKRIQFTPDLVPADLIGTRIYNQKTGEFNTSLGPV
                     FTNLLLADEINRAPAKVQSALLEVMQERQVTIGRETFKVPNPFLVLATQNPIETEGTY
                     ALPEAQVDRFMLKVLVGYPTQTEEFVIVERMTGAVQAIQRVLTTDELVELQKKVDQVY
                     VDPALIEYVVKLVNATRSPKTVGLDDMEHYITFGASPRASINLVLTAKALAFVRGRTY
                     VLPQDVLDMALDVIRHRLVLSYEALSDEVSSDDILSKILDRIPVPVVPLNEHFNVRAN
                     S"
     gene            complement(2016..2855)
                     /locus_tag="PLT08_11245"
     CDS             complement(2016..2855)
                     /locus_tag="PLT08_11245"
                     /inference="COORDINATES: protein motif:HMM:NF024758.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HNE05086.1"
                     /translation="MSESTETNQERFKKGIKRIASFLRRGLPFASGVFAALLAFILYN
                     LVIVQPNQLSVEDVNASVASAMGSATPPPAYSAQVYEVIRPSLVLIQTEEKHQDAESD
                     FGLGSGVVVDAFGDILTSRHVIDGATRIKITFADGTESEAQVVSEMPELDLAVIQALD
                     SPPVLYPAILGNPGAMRVGDEAFVVGNPFGLYGSMSSGVISGFDRVFRIPDSNIEIPG
                     MIQFDAAANPGNSGGPLLNRQGHVIGIVTGLTNPTDENFFVGIAFAVPINIAVGGMGS
                     PPY"
BASE COUNT          750 a          801 c          763 g          683 t
ORIGIN      
        1 gaccaaagca tactttgcca aatgaaactc atcgggtcat ttcactttcg gcgctgacgg
       61 cgcaacatca caaaacttgc aatcgaatgg atcaagtctg cttcggtgga aagcgaaagc
      121 acgtctacac cggcacgttt gaacgaatca gccaaggctg cttcgcgctt attcgccgct
      181 tccataaaac gctgacggaa tttcttatcg ctggtatcca cgaacaattg ctcgccggtc
      241 tctgcatctt ccaacacaat gtgaccgaca tctggcagcg tcgtctcgcg cgggtcccat
      301 aaacggatgg cgatgacctc atggcgttgc gccaacaaac tcaatgaacg ctcccagcct
      361 ggtgtgctga tgaaatcaga caccacaaac acaagtgaac gcttcttgat actgtgcaac
      421 ccaccgcgaa tcaaagcgga taaatccgtc atcggcacac gcgtgagacg cggctgtttg
      481 agcagatcgt taatgaggcg cagaacatgc atgcgcccgc ctcttgctgg aaccgtatgc
      541 tggacaccgc tcccgtacat caccgccccg acgcgattcc cgtgacgtgt gagtaagcgt
      601 gataacacgc ccacaaagtc gaccagaacc gtgcgcttct gtgtatcgag cgtgccaaaa
      661 tccacagacg gactcaaatc cagcaagaac caggcgataa tctcgcggtc ttccatatac
      721 tcgcggacgt agggcgtatc catgcgcgcg ctgacgttcc agtcaatgta gcggatgtcg
      781 tcttgcggct ggtattcgcg caagtcggca aagtccacgc cagagccgcg gaagagactg
      841 cgataatcac cctgcaaata tccgtccaga cggcgaatga cctgccaatc cagccggtgc
      901 agaatgtgct caggagttcg cgcggacgtt gaagtgttca ttcaatggca ccacaggcac
      961 gggaatgcgg tcgaggatct tgctcaaaat atcatcactg ctcacttcat cagagagtgc
     1021 ttcatatgaa agcaccagac gatgacgaat gacatccaac gccatatcga gcacatcttg
     1081 cggcaagacg taggtacgtc cgcgcacgaa agcgagcgct ttggcagtca acaccaaatt
     1141 gatggatgca cgcgggctgg caccaaaggt gatgtaatgt tccatatcat ccaaccccac
     1201 ggtcttcggg ctacgcgtgg cattgaccag cttgaccaca tattcgatca acgccgggtc
     1261 aacatagact tggtctacct tcttttgtaa ttccaccaac tcatcggttg tgagcacgcg
     1321 ttgaatggct tgcacggcac cggtcatgcg ctcgacgatc acgaattctt ccgtttgagt
     1381 tgggtagccc accaaaacct tgagcatgaa acggtccact tgggcttcgg gcaaagcata
     1441 agtcccttcg gtttcaatgg gattctgagt cgccaacacc aagaacggat ttggcacttt
     1501 gaaggtctca cgtccaatcg tcacctgccg ttcttgcatc acttcgagca aggcactttg
     1561 cactttggca ggcgcgcggt tgatttcatc ggcaagcaac aagttggtga agaccggacc
     1621 caaagacgtg ttgaactcac ctgtcttttg gttataaata cgcgtaccga tcaaatcagc
     1681 ggggacaagg tctggggtga attgaatacg tttgaattca ccgccgatgg agtcggcgag
     1741 ggttttgatc gccatggtct ttgccaagcc cggcacacct tccacaagga tatgtccgcg
     1801 tgcgagcaag gcgacgacca agcgttccaa taaatggtct tgcccaacga taactttctt
     1861 gacttcgtac aatacccgtt ccatcggaag ttgattctcg cgatttgttt gttccatgct
     1921 tggtctcctt attatggtct cctaagtaag tgcgaccctt ttttaagata agaatgtttt
     1981 gctcccgccg ggcgggtcgc ccctacgatt tatgcctaat acggcggcga tcccatacca
     2041 cccaccgcaa tattgatggg aactgcaaag gcaatgccta caaaaaaatt ttcgtctgtg
     2101 gggttggtta aaccggtcac gatgccgatc acatgtccct gccggttaag cagcggacca
     2161 cccgagttgc cggggttggc ggcagcgtcg aattggatca tgccggggat ttcaatattg
     2221 ctatcgggaa tgcgaaagac ccggtcgaag cccgagatga ctcccgaact catcgagccg
     2281 tacaagccga acgggttgcc taccacaaag gcttcgtccc ccactcgcat cgcgccgggg
     2341 ttgcctaata tcgctgggta tagcacgggc ggagaatcca aggcttggat gacggcgagg
     2401 tctagctcgg gcatttcaga aacgacttgc gcttcagact cggtgccatc ggcaaaggtg
     2461 atcttgatgc gggtcgcgcc atcgatgaca tgacgactgg tgaggatatc gccaaatgca
     2521 tcgacgacca cgccgcttcc caagccgaaa tctgattcag cgtcttgatg cttttcttct
     2581 gtttgaatta ataccaacga cgggcggata acttcataga cctgtgccga atatgccggt
     2641 ggcggagtcg cagaccccat ggcagatgcc accgatgcgt tgacatcctc cacgctcagt
     2701 tgattgggtt ggacgatgac gaggttatag agaatgaaag ccaaaagggc agcgaaaact
     2761 cccgaagcaa aaggcaagcc tcggcgtaag aaagaagcga tgcgttttat cccctttttg
     2821 aatcgctctt gatttgtctc agtcgattcg ctcatgcaaa agaatcctta tctaaagtag
     2881 ttggagcgga cgcgaacttg tttgagtact ataaggtgct tattttagct aaagatgaga
     2941 gaaatggttg tattcttaca gtgcaaagga gtgttcacaa aatacacaca attataa
//
LOCUS       DAOQJE010000427         2995 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_22191_length_2995_cov_6.198299, whole genome shotgun sequence.
ACCESSION   DAOQJE010000427 DAOQJE010000000
VERSION     DAOQJE010000427.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2995)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2995)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2995
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22191_length_2995_cov_6.198299"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1456
                     /locus_tag="PLT08_11250"
     CDS             <1..1456
                     /locus_tag="PLT08_11250"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNE05087.1"
                     /translation="KVMSKQDLILLALDASPTLDLMVRAFHSANYEVAVVHDQSGLEK
                     SIQQAIPALMIIGEIFNGEDGFSIATNTLERFPTMPIILYAEKDKPGLAQHIIKAGIS
                     GYLYPPLKLDDIVEEVKRTLIRARKLGDWLRREVKRTTASLEQKAQISEAERNKLDAI
                     ISNIQDGVLVLDETHHILLANKTIYEIFSLNGNPILGKYVKDVINSADLNALIDRARN
                     GALKYHEINFDDGRVYNAQYTPLPRIGSAVTMQDISYLKEIDRLKNDFVHTVSHDLRS
                     PLTSVLGYMELIERVGPLNESQHEFLERLRAGVHHMTSLVNELLDLGRLESGFDTRRE
                     TVNLRNVLEYSLNVFDNQIKKKNLQVTCEFADNLKSLRANPIRIRQMIDNLVGNAIKY
                     TPQNGAIKLNLAMQETQVVFRVTDSGPGIPPDEQNRVFEKFFRASNRSSEIEGSGLGL
                     AIVKSIVESHQGRVWVESKVGIGSTFIVLLPATE"
     gene            complement(1524..2876)
                     /locus_tag="PLT08_11255"
     CDS             complement(1524..2876)
                     /locus_tag="PLT08_11255"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HNE05088.1"
                     /translation="MFLANEYYPAYITASLELDKGMHLVCTADSSRPALLNDQGWELF
                     SRIVNQQPITGSLAGTACGSFGQSLIAAGLLLPGSRNATPKEQNHILTSWLHLTDRCN
                     LRCSYCYLPHVLEDMSLKTGYVAMDAIFRSALAHGFKQVKIKYAGGEPLLCFATLITL
                     HTYAQQLAEKHRLDLDGVILSNGTLLTVEMVEMLKTFDLRLMISLDGLGNYHDSHRPY
                     AGGRGTFADVSEAIDLALAHGVVPDISVTVSNRTADGLANVIAWILDRNLPFSLNFYR
                     ENELSISHTDMQLKDEKIIHGVLAAYKVIEKQLPQRSLLAALIDRANLAVPHIHTCGV
                     GQNYLVFDQNGKVAKCQMQIHKPLTDLDAADPLALIRADQGGIQNLPVMEKEGCKTCE
                     WKHWCTGGCPLETHRVTGRYNVKSPNCNIYKALFPEALRLEGLRLWKYQDNPEVVTKI
                     "
BASE COUNT          849 a          688 c          660 g          798 t
ORIGIN      
        1 gaaagtaatg tcaaaacaag acctgatttt gctggcattg gatgcatcgc ccacgttgga
       61 tttgatggtg cgtgctttcc actccgcaaa ttatgaagta gcggtagtcc acgaccagtc
      121 aggattagag aaatcaatcc aacaagccat tccagcattg atgatcattg gggaaatttt
      181 caatggagaa gatggctttt caattgccac aaacacacta gaacgttttc ccaccatgcc
      241 catcattctc tatgcagaga aagataagcc tgggctggct caacatatca tcaaagcagg
      301 cattagtgga tatttatatc cgcctctaaa actggatgat attgttgaag aagtaaagcg
      361 cactcttatc cgcgcccgaa agctgggaga ttggcttcgc cgcgaagtaa agcgcacaac
      421 ggcttcgttg gaacaaaagg ctcaaatctc agaagcagaa cgaaacaagt tagatgcgat
      481 catatctaac attcaagatg gtgtattagt cttggatgaa acgcatcata ttctgttagc
      541 aaacaaaacc atctacgaaa tctttagtct caacgggaat cccatccttg gaaagtatgt
      601 caaagatgtg attaacagcg cagacttaaa cgctctgatt gaccgtgctc gaaatggcgc
      661 gttgaaatat catgaaatca actttgacga tggacgcgta tacaacgcac aatacactcc
      721 tctgccacgc atcggcagtg cagtgaccat gcaagatatt tcatacttaa aagaaattga
      781 ccgtttgaag aacgattttg ttcacacagt ttctcatgat ttgcgctccc cactgacctc
      841 tgttttggga tacatggaac taattgaacg agtgggaccg ttgaacgagt cacagcatga
      901 gtttctggaa cgcttgcgag cgggtgtaca tcacatgacc agcttggtta atgagttact
      961 cgaccttgga cggctcgaat caggttttga tacccgccgc gaaacggtaa accttagaaa
     1021 cgtgcttgag tactctttga acgtgttcga taaccagatc aaaaagaaga atctccaagt
     1081 tacctgtgag tttgcagata atctcaagtc actccgagcg aatcccattc gcattcgcca
     1141 gatgatcgac aacttggtag gtaatgctat caagtacaca ccacaaaatg gtgccattaa
     1201 attaaacctt gctatgcaag agacacaggt cgtctttcgc gtcacagaca gcggtccggg
     1261 catcccaccc gatgaacaaa atcgtgtatt cgagaagttc tttcgcgcat cgaaccgatc
     1321 cagcgaaatt gaaggctccg gtttggggct ggcaatcgtc aaatccatcg tagaaagcca
     1381 tcaagggcgg gtatgggtgg aatcaaaagt aggtattggt tctacattta ttgttcttct
     1441 ccctgcaact gagtaatgca ttttacaaaa acaaaagaag gctgatggtt accagctttt
     1501 ttccattaac tacgaaactc cttttatatt ttggtcacta cttcagggtt atcttggtat
     1561 ttccacaatc gtagaccttc aaggcgcaag gcttctggga atagtgcctt gtaaatattg
     1621 cagttcggcg atttaacgtt ataccgtcca gtcacgcgat gggtctcaag tggacaacca
     1681 ccagtgcacc aatgcttcca ttcgcaggtc ttgcagcctt ctttttccat tactggcaaa
     1741 ttttggatac caccttgatc agcacgaata agcgccagcg gatctgcggc atccaaatct
     1801 gttaacggtt tgtgaatctg catctgacat ttcgctactt tcccattctg atcgaagaca
     1861 agataattct gccctacacc acaggtatga atatgtggga cagcgaggtt tgccctgtcg
     1921 atcaacgcgg cgagcaagct tcgctgcggt aattgctttt caatgacctt ataagcagca
     1981 agaactccat ggattatctt ttcatccttc aattgcatat ccgtatgaga tatggacaat
     2041 tcattctcac gataaaagtt caagctgaat ggcagattac ggtccagaat ccatgcaata
     2101 acattcgcca atccatctgc tgtgcggttg ctgactgtta ctgaaatgtc tggtacgact
     2161 ccatgcgcca atgccaggtc aatggcttct gatacatctg cgaatgtccc tcgtccgcct
     2221 gcatagggtc ggtgactatc gtgataatta cccaatccat cgagagaaat catgagacgc
     2281 aaatcaaatg ttttcagcat ctcaaccatc tccactgtca ggagggttcc attacttaag
     2341 ataacaccgt ccagatcgag tctatgcttt tctgcaagtt gttgagcata ggtatgtaag
     2401 gttattaatg tcgcgaaaca caagagcggt tcaccacctg cgtatttgat cttgacttgt
     2461 ttgaatccat gtgccaacgc cgagcggaag atggcatcca tggcgacata acccgtttta
     2521 agtgacatat cctcaagcac atgtggaaga tagcaataag aacaacgtaa attacaacga
     2581 tctgttaggt gcagccaaga tgtcaaaata tgattttgtt ctttgggtgt tgcatttcta
     2641 cttccgggta ataaaagtcc ggcagcaatc aacgattgtc caaaacttcc acaggctgtg
     2701 cctgccaacg agcctgttat cggttgctga ttcactatcc gggaaaatag ctcccagcct
     2761 tggtcattta aaagagccgg gcgtgaagag tcagccgtac agacaagatg catcccttta
     2821 tctaattcca gcgaagctgt tatgtaagca ggataatatt catttgcaag aaacatattg
     2881 cccttttcag ccaagatcgg taacgtggga gggcatgcgt cgcactggcc cgtggcacat
     2941 gccacgggcc gatggtcaaa gataacactg ggtgtttctt gggttgcaaa tttat
//
LOCUS       DAOQJE010000428         2992 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_22230_length_2992_cov_6.098400, whole genome shotgun sequence.
ACCESSION   DAOQJE010000428 DAOQJE010000000
VERSION     DAOQJE010000428.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2992)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2992)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2992
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22230_length_2992_cov_6.098400"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..899
                     /locus_tag="PLT08_11260"
     CDS             <1..899
                     /locus_tag="PLT08_11260"
                     /inference="COORDINATES: protein motif:HMM:NF013720.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE05089.1"
                     /translation="RGFDNFLVVVNASNDDKDRTWLESVRDGKVKIDNARSWARAYGY
                     NAEIRNLRDPKSGNAMRVDIALQGPKSRDTLLAMGVDDATRARIMKLKRTELCDANVG
                     GFDLIVSRTGYTGEKMAFELFVHPERAVDFWNGVMKAGEPFGVKPIGLGARDSLRTEA
                     GLPLYGHEMGLGSGKFDGRDLGVAEGGFGSYVKTYKPWFIGRDAFVAREKERKSVVVR
                     FTFDEQRTRMAHNGDPVVNANGERIGFVTSCAIDNARFITGQAFVDLAYAKEGTVIGV
                     NQGGNTDRPAGLAKVVSRFAKL"
     gene            1004..1639
                     /locus_tag="PLT08_11265"
     CDS             1004..1639
                     /locus_tag="PLT08_11265"
                     /inference="COORDINATES: protein motif:HMM:NF022440.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2806 domain-containing protein"
                     /protein_id="HNE05090.1"
                     /translation="MSDNNLPLLDIGKLSEPATKLIQVVADAVGVIYEPTRIKRKAKA
                     DAEAEIIKTKAKLQSDTLKLRAKKRKQTNELKQQKNIESIIEKSIPQLPESVSSQPVD
                     DDWVVQFFNFAQDVGNDEMQVIWARLLAGEVANPGSYSLRALHAIRMLNPTDAIYFAK
                     FCNYLWDRKEYFFTEETQKHLDKHGLSDYVIFHLKTLGLITTELDFPLKGA"
     gene            complement(2173..2520)
                     /locus_tag="PLT08_11270"
     CDS             complement(2173..2520)
                     /locus_tag="PLT08_11270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE05091.1"
                     /translation="MQSEKSNFTNIERLIAVHGNRFILHRILHALPLDKFDMIDLDMS
                     PIYKQAAQETLSELEGLHDVIRLHFPNEYLNTLFKSRSKCEQLVSFLPKSLPIPKTKY
                     FSKSEDLQPPLFN"
     gene            complement(2623..2922)
                     /locus_tag="PLT08_11275"
     CDS             complement(2623..2922)
                     /locus_tag="PLT08_11275"
                     /inference="COORDINATES: protein motif:HMM:NF022062.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIPR family protein"
                     /protein_id="HNE05092.1"
                     /translation="MVRFISLENCPPDFAVEVTTATNTQNKIELRDFASLDPTQERLR
                     QDLVLDLQKIYAYKSGEVISSPQDGCTIDEATIALACSLPEVQFAADAKRYISQL"
BASE COUNT          858 a          582 c          721 g          831 t
ORIGIN      
        1 gccgcggatt tgataacttc ctggtggtgg tcaacgcgtc gaacgatgac aaggaccgca
       61 cctggttgga aagtgtgcgc gatggcaagg tgaagattga taacgctcgc tcgtgggcgc
      121 gcgcgtacgg ctacaacgcg gagatccgca acctgcgcga cccgaaatcg gggaacgcca
      181 tgcgggtgga cattgccctg caaggaccga agagccgtga cacactgctt gccatgggcg
      241 tggacgatgc gacgcgtgcc cgcattatga agttgaagcg cactgaatta tgcgatgcga
      301 acgtgggtgg ctttgacctg atcgtttcgc gcacgggtta taccggcgag aagatggcct
      361 tcgagttgtt cgttcacccc gagcgcgccg tggacttttg gaacggcgtc atgaaagcgg
      421 gtgaaccctt cggcgtgaag ccgatcggtt tgggtgcgcg tgactcactg cgcaccgaag
      481 cgggcttgcc gttatatggt catgagatgg gcttgggttc cggtaaattt gacggacgcg
      541 atctgggcgt tgccgaaggc ggatttggtt cgtatgtgaa gacctacaag ccgtggttca
      601 tcggacgcga tgcctttgtg gcgcgtgaga aggaacgcaa gagtgtggtg gtgcgtttca
      661 ccttcgacga acagcgcacg cgcatggcgc ataacggtga cccggtggtc aatgccaacg
      721 gcgaacgcat cggctttgtg acctcctgcg ccattgacaa cgcgcgcttc attaccggcc
      781 aggccttcgt ggacctggct tatgccaagg aaggcacggt catcggagtc aatcagggcg
      841 gcaatacgga ccgccccgct gggttggcga aggtggtgag tcgcttcgcg aagttgtagg
      901 agaagaaaga tgggacggcg tcagggcggg acgccgtccc attgtgatga agaagagatt
      961 atctaattgt taggatagca atctcataga aagggtgttt attatgagtg acaacaattt
     1021 gccattactt gatattggaa aacttagtga gcctgcaact aaactaatac aggttgttgc
     1081 tgatgctgtt ggagtaattt acgagcctac taggataaag aggaaagcta aagctgacgc
     1141 agaagcagag ataattaaga caaaggcaaa attacaatct gacaccttga aattgcgagc
     1201 taaaaaaaga aaacaaacca atgaattgaa gcagcaaaaa aatatcgagt caattatcga
     1261 gaaatctatt ccacaattgc cagagagtgt ttctagccaa ccagttgatg atgattgggt
     1321 cgttcagttt ttcaactttg ctcaagatgt cggcaatgac gaaatgcaag taatttgggc
     1381 tcggctattg gcaggtgaag tagctaatcc aggatcatat tcacttcgcg cactacatgc
     1441 aattcggatg ttgaatccaa ctgatgcaat ttatttcgcc aagttctgta attatttatg
     1501 ggatagaaaa gaatatttct ttacagaaga gacacagaaa catcttgaca aacatggcct
     1561 cagtgactat gtcatctttc acttaaaaac gttggggctt ataactactg aattagattt
     1621 ccctctcaaa ggagcttaaa ctatcgcact aaattatttc gacagaaaat atgcaataag
     1681 caataaaagt ttcagtgatc ataacttaag agtttttata ttaactgaat taggaaaaga
     1741 aattgcaact ttatgtgatg taaaacctga ttatgaatat tgtgaaatag tggtgtcgaa
     1801 atgggacatt gaaaatatat atgtagagag aatttaggat aattgctagt ttcttgaata
     1861 gtactggtcc ttaccctaat ttacatcata atcaagtttc tctgattgtt tccctttaag
     1921 agagagtgtt ttttgttgct gctctttgaa acaatcacca aaagtatagt taaagagcaa
     1981 ttatcatgta taaaaataaa agaaaatcaa accgcctttg aggactaaat tttctgcgta
     2041 ttcggttctg gataggcgat acagaacctt ccccatgcca taatgttgca gcaagtcatt
     2101 gaacgactta cggctttttc gggctttgtt ggcaatctgc gcatgaattc ccatcttttt
     2161 tgatcttcta aactaattaa agaggggagg ctgtaaatct tcgctctttg aaaaatattt
     2221 tgtcttagga atgggaaggc ttttgggcag gaaactaaca agttgctcac attttgaacg
     2281 gctcttaaat aatgtattta aatattcatt tgggaaatgc aatctaatta catcatgcaa
     2341 cccttctagt tctgatagag tttcttgtgc agcttgttta tatattggtg acatatctaa
     2401 atctatcatg tcaaatttat cgagaggtaa agcatgaaga attctatgta aaataaatct
     2461 gttgccatgc actgcaatta atcgctcaat attagtgaaa ttactctttt cggattgcaa
     2521 agtgctttca acaattctag aaatctcaac agagcgccat aaacgatatg cagagaggtt
     2581 ttcgttgaaa attaacgtat aagggggttc gtgtatatct ccctaaagtt gactaatata
     2641 tctctttgca tcagcagcaa actgtacttc aggcaatgaa caagcaagcg caatcgttgc
     2701 ttcatcaata gtacatccgt cttgtggcga tgaaattact tcaccgcttt tgtatgcgta
     2761 tattttttga aggtctaaca caagatcttg tcttaatctt tcctgggttg gatcaagaga
     2821 tgcaaaatct cgaagttcaa ttttattttg agtatttgtt gctgttgtaa cttcaaccgc
     2881 aaaatcgggc gggcaatttt ccaaagaaat gaagcgcacc aaaacacgcc catttcctga
     2941 cctgctttag ttattgtgtc caactggaat gagagtcaga gcaggttgga tt
//
LOCUS       DAOQJE010000429         2990 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_22254_length_2990_cov_5.403748, whole genome shotgun sequence.
ACCESSION   DAOQJE010000429 DAOQJE010000000
VERSION     DAOQJE010000429.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2990)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2990)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2990
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22254_length_2990_cov_5.403748"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            259..1185
                     /locus_tag="PLT08_11280"
     CDS             259..1185
                     /locus_tag="PLT08_11280"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: protein motif:HMM:TIGR00745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008677 - 2-dehydropantoate 2-reductase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HNE05093.1"
                     /translation="MKIAVFGTGGVGGYFGGRLAQHGQDVTFIARGAHLVAIRDAGLK
                     VESVDGDFTVHPVNVTDSPKTVGSVDLILLAIKAWQLEDAIEQMKPMVGESTVILPLL
                     NGMEHMDSLFAQFGAHVIGGFCRISAFIGSPGVICHVGVKPYIAYGEWDNSKSERIQS
                     IHDLFRSISGIVAEIPVDIQTAMWEKFIFIAGTSGVGAYTRQTIGEYRANPETRAMLQ
                     HAMQETSAIAYAKGVNLAADFVDFTMKRIDTIDPNITASTQKDIMAGKPSELNQQTGA
                     VIRMGKAVGVATPTHEKIFAALLPFEQKARAQ"
     gene            1280..2182
                     /locus_tag="PLT08_11285"
     CDS             1280..2182
                     /locus_tag="PLT08_11285"
                     /inference="COORDINATES: protein motif:HMM:NF012318.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HNE05094.1"
                     /translation="MSKNIFSELSEAMANAAEKAGKYTVLVDARRRMPASGIAIDKET
                     ILTAGHVVEREEDIKILFADGKEVTARLIGRDPGTDLAVLKLDSAVATPAELAKTPAR
                     AGQFVLAVGRPSANGIESSFGTVNKISGPVRTGRGGMLEKYIKTDVVSYPGFSGGPLI
                     NGDGEVYGINTSGFGMGGEAITIPADLAWKIAETLTKHGKIKRGFIGVRSQTVNLPAD
                     AQAQLNREQETGLLLVGIEKDSPAEKGGLIIGDILVGVAGVAVEHHDELFTRLSGDVV
                     GKPTAMDVLRGGKLQSVSVVVGER"
     gene            2242..2847
                     /locus_tag="PLT08_11290"
     CDS             2242..2847
                     /locus_tag="PLT08_11290"
                     /inference="COORDINATES: protein motif:HMM:NF012422.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HNE05095.1"
                     /translation="MIRVTIVSPNHALRVGLREMLANQAGIRVVGEATRLSDVNERET
                     EVAVLASVSSVRSVDQKDFCILFLTDDIESVRPLLSSGMSAWGVLSSEANADELAAGI
                     AAVAEGLWVGAPGLVEGLMRLPKGGEGMGEESLPEPLTAREKEVLQHMAEGLANKQIA
                     LALRISEHTVKFHLSALYAKLGAASRTEAVKRGIELGLISL"
BASE COUNT          786 a          651 c          806 g          747 t
ORIGIN      
        1 ctgttgtgga aacagatagc gaacccgaag ttgatgaacc cgttgtaaaa cgaaagtctc
       61 ctgctcgggc gaaggctaca acagaaaaga aaccaaccac taagaaagca cctgccaaac
      121 gtaagaaggc agagtaaaac ctgatttaca aacaaaaaag ctgatgatac ccatcagctt
      181 tttgtttgta aaagtaagta agggcggtgt atagttgctt atcgaacata ataagttctg
      241 ctagttggag cataacaaat gaaaattgca gtatttggca caggcggtgt gggcggatat
      301 ttcggcgggc ggctggctca gcatggacag gacgtcacct ttattgcgcg aggagcgcac
      361 ttagtagcta ttcgtgatgc aggattaaaa gtcgaatctg tagacgggga tttcacggtt
      421 cacccggtca atgtaacgga ttctccaaag acagtgggca gcgttgattt aattttgctt
      481 gccatcaaag cctggcaatt ggaggacgct attgagcaaa tgaaacccat ggttggagaa
      541 agtacagtca ttttgccttt gctcaatggt atggaacaca tggattcgtt gtttgctcag
      601 ttcggcgcac atgtaattgg tggcttttgt cgtatcagtg ctttcattgg cagtcccggt
      661 gtgatttgcc acgtaggcgt caaaccatat attgcctatg gcgaatggga taactccaag
      721 agtgagcgca ttcagtctat tcatgatttg tttcgttcaa tcagtggcat tgtggcagaa
      781 attcctgttg atattcaaac cgcaatgtgg gagaaattca tctttattgc tggtacaagc
      841 ggggtaggag catacacgcg acagacaatt ggagaatatc gcgccaaccc tgaaacgcgc
      901 gctatgcttc aacatgccat gcaagaaact tcagccattg cttatgcaaa aggtgtgaat
      961 ctagcggctg actttgtaga tttcaccatg aaacgtattg atacgattga cccaaacata
     1021 acggcatcca cgcagaagga tatcatggcg ggcaaacctt ccgagcttaa tcagcaaact
     1081 ggcgcggtca ttcgtatggg caaggcagtg ggtgtggcta ccccaaccca tgaaaaaatt
     1141 tttgcagcct tgttgccgtt cgaacaaaaa gctcgtgcac aataatgccc actagccgaa
     1201 tgtctagtat cgccccagtc aattcaccga tacactccaa acacatgctc gctagaatgg
     1261 gcataaagga gtaacgaaaa tgagcaaaaa tatttttagt gaactttctg aagccatggc
     1321 aaatgctgcc gagaaggctg gcaaatatac agtcttggtc gatgcgcgcc gccgcatgcc
     1381 tgcaagtgga attgcaattg ataaagaaac gattctgact gccggtcacg tagtagaacg
     1441 cgaagaagat atcaagattt tattcgcgga tggaaaagaa gtcacggcac gcctcatcgg
     1501 tcgggatccg ggaacggacc tcgccgtctt gaaattagac tccgctgtag ccactcctgc
     1561 cgaactcgca aagacgccag cacgtgcggg gcagtttgtc ctcgctgtgg ggcgcccttc
     1621 cgcgaatgga attgaatcca gctttggcac cgtgaacaaa attagcggac ccgtccgcac
     1681 cgggcgcggt ggcatgttgg agaaatacat caagaccgat gtggtctctt accccggttt
     1741 ctctggcgga ccactcatca acggcgatgg cgaagtgtat ggcatcaaca cctctggctt
     1801 tggcatgggc ggcgaagcaa ttaccatccc tgctgatttg gcatggaaga ttgcagagac
     1861 ccttaccaaa catggcaaga ttaaacgcgg ctttatcggt gttcgcagtc aaaccgtaaa
     1921 tctccctgcc gatgcgcaag cacaacttaa ccgtgagcaa gaaacaggtt tgctgttggt
     1981 tggcattgaa aaagacagtc ccgcagaaaa aggcgggctg atcataggcg atattcttgt
     2041 cggtgtcgct ggcgttgccg tggagcacca cgacgaatta ttcacccgtt tgagcggcga
     2101 tgtagttggc aagcctactg ccatggatgt tcttcgtggt ggtaagttgc aatcggttag
     2161 tgttgttgtt ggcgaaagat aaaaatataa agagcacaga gcataaaact ctgtgctctt
     2221 tatcgcaaga aaggttttat tttgatccgt gtaaccatcg tctcaccgaa tcatgctttg
     2281 cgggttgggt tgcgtgaaat gcttgccaac caagcgggca ttcgtgtggt gggggaggcg
     2341 actcgtcttt cagatgtcaa tgaacgcgaa accgaggtgg ctgtgcttgc ctcggtttct
     2401 tctgtacgta gtgtagatca aaaagatttt tgcattcttt ttctgacaga tgatattgaa
     2461 tccgtacgac ctttattatc atcggggatg agtgcgtggg gggtcttatc ttctgaagcc
     2521 aatgccgatg aattagcagc gggaatcgct gcagtggcag aaggcctttg ggtgggcgca
     2581 cctggcttgg tcgagggctt gatgcggttg ccaaagggcg gcgaaggcat gggagaagag
     2641 tctttaccag agcctctcac ggcacgcgag aaggaagtcc tccagcatat ggcggaggga
     2701 cttgccaaca agcaaattgc gctggcgttg cgaattagcg aacatacggt taagtttcat
     2761 ctttcggcgt tgtatgccaa attgggggcg gcaagccgca ccgaagctgt gaagcgcggc
     2821 attgagttgg ggttgatttc gttgtaattt cttatttgtc tgtaatgctg ttgacaggtt
     2881 tggcaggtaa aataatgaga ctttataggt ctatatatca tgccaaacga atttcactac
     2941 aaatgggaat gggacttggc gtcttcgccg gaagcattgt ggccgcttgc
//
LOCUS       DAOQJE010000430         2988 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_22287_length_2988_cov_3.138766, whole genome shotgun sequence.
ACCESSION   DAOQJE010000430 DAOQJE010000000
VERSION     DAOQJE010000430.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2988)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2988)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2988
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22287_length_2988_cov_3.138766"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            16..177
                     /locus_tag="PLT08_11295"
     CDS             16..177
                     /locus_tag="PLT08_11295"
                     /inference="COORDINATES: protein motif:HMM:NF014764.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE05096.1"
                     /translation="MHAHFYPPLLRSATVRKFMVGYEMLGMPQRDITPETAAERLRAV
                     SNIHYKSQR"
     gene            complement(227..1285)
                     /locus_tag="PLT08_11300"
     CDS             complement(227..1285)
                     /locus_tag="PLT08_11300"
                     /inference="COORDINATES: protein motif:HMM:NF021539.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2270 domain-containing protein"
                     /protein_id="HNE05097.1"
                     /translation="MPRKKTTLPPQPPQEEDSVWTYRGYKLKSSEFVTAMVHLFRAEV
                     QRANVWRQRLDTTTNWAVVATGATLSISFSQPNVHHGVIILNTLLVTWFLFIEARRYR
                     YYELWSYRVRLMETDFYAPMLVPPFHPSPEWAENLAENLLTPSFPISMWEAFGRRLRR
                     NYLWIYLILFISWMGKSFLFPEPATTISEFIDRGAIGAIPGYIVISLGLVYHLFLLII
                     AIGTVGMTKATGEVLPRFGDETAPAAQSMEGKHTGLRALLVPRRKRRQLLALIITDKA
                     KGVSSRIMTDLKRGVTSMQGKGMYTGKKRSILMCALTITEAHALKAAVAKEDPKAVVI
                     VSPAQEILGGGFAPLDGK"
     gene            complement(1291..>2988)
                     /locus_tag="PLT08_11305"
     CDS             complement(1291..>2988)
                     /locus_tag="PLT08_11305"
                     /inference="COORDINATES: protein motif:HMM:NF015557.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HNE05098.1"
                     /translation="ALGLSGILTPQEAFSGFSRQAVITMLSAFILAEGLRRSGMTERI
                     GLFIIKLFGAGERRLIFGVMTASAVLSLFMNNIAAASLLLPSISGIAHKMKISPSKLL
                     MPLAFGTILGGMATLLTTVNLVVSGLLRDENLPAFSLTDFLPMGLTLTFAGILFMVLW
                     GRKLLPNQSPAQRHEEENASVELIKTYQLAERLVRAEVCKGGRMDGVTLEKSGLREKY
                     HLNVIAIQRRRNTLPIEAKTIIKGDDILLIVARPEDSLPQSLGEMLNILPSGQWEQDY
                     LSTPNMKLIEAAIAPRSHLASQTLSEIRFEQKFNSKVLAIWRHGRSIRTRLADLPLEF
                     GDGLLMQGTEKALSLLQTEPGLILLAESAPSTRMTWRNWLTVLIMFATMVLAVVFSDN
                     IAEIMLTGSVMMVLIGTMNMDQAYRSIDWRSLFLVAGMLPLGVALNKTGASTLFANAI
                     LAELGDANHLVLLASFVLLTVVLTQIINGAAAVTVIAPIAIATSLQVGMEPRSVAMAV
                     ALASSMAFMSPLGHSVNVMVMGAGGYTFKDYARVGIPLTILLVIILLFVLPILMPIG"
BASE COUNT          861 a          750 c          660 g          717 t
ORIGIN      
        1 caccccgaat gggtattgca cgcacacttt tatccaccct tgttgcgctc cgccacggtc
       61 cgcaagttta tggttgggta tgaaatgctt ggcatgccgc aaagagatat cactcctgaa
      121 accgcagccg aacgattacg agccgtttca aacatccact ataaatcaca aaggtaagat
      181 actaaaaagg tgctggagtt ttctccagca cctttttagt agtctattat ttcccatcta
      241 atggagcaaa accgccgccc agaatttctt gtgcaggcga cacgatcacg acagctttag
      301 ggtcttcctt ggcaaccgcg gctttgagtg catgcgcttc ggtaatggtc aatgcacaca
      361 tcaaaataga tcgttttttg ccagtataca taccttttcc ttgcatggat gtcactccac
      421 gtttcaagtc ggtcatgatt cggctggaaa cgccttttgc cttatcggta ataatgagag
      481 ccagcaactg cctgcgcttt ctacgtggaa caagcaatgc gcgcaacccc gtatgtttgc
      541 cttccattga ctgagcagca ggggctgttt catctccaaa gcgaggcaaa acttctccgg
      601 tagctttggt catacccact gtgccaatcg caatgatcag cagaaaaaga tgatatacca
      661 atccaagtga gataacaata tacccaggga tggcgccaat cgcgccacgg tcaataaatt
      721 ctgaaatggt tgtcgctggt tcagggaaaa gaaaactttt ccccatccaa gaaataaaaa
      781 gaatcaagta gatccacaga tagttgcgtc tcaaacggcg accaaatgcc tcccacatag
      841 aaatgggaaa gcttggcgta agcaaattct cagcgaggtt ttcagcccat tcaggtgatg
      901 gatgaaatgg cggcacaagc atcggcgcat aaaaatcggt ttccatcaag cgcacgcggt
      961 agctccatag ctcataatag cgataccggc gggcttcaat aaataggaac catgtaacta
     1021 atagtgtatt taaaataata acgccatgat gtacattggg ttggctaaaa gaaatggaaa
     1081 gcgttgcgcc agtggcaacc accgcccaat tggtagtggt atccaagcgc tgacgccata
     1141 catttgcacg ttgcacttct gcgcgaaaaa gatgcaccat ggcagtgaca aattcactgc
     1201 ttttgagttt gtatccgcgg tacgtccaga cagaatcttc ttcttgtgga ggttgaggag
     1261 gtagggttgt cttttttctt ggcataaagt ttatccaatc ggcataagaa tggggagaac
     1321 aaacaaaaga ataatgacaa gcaaaatcgt aagagggatg cctacccttg cgtaatcttt
     1381 gaaggtatat ccaccagcgc ccattaccat aacattgacg gaatgcccaa gtggtgacat
     1441 gaatgccatt gaagaagcca atgctaccgc catggctaca ctgcgcggct ccatcccgac
     1501 ctgcagactg gtggcaattg cgatgggagc gattacagtg acggcagcag caccattaat
     1561 gatctgggtc aacacaaccg taagcaaaac aaaactcgcc agcaatacca ggtgatttgc
     1621 atccccaagt tcagctagaa tagcatttgc aaacagcgtg gatgctcctg ttttattcaa
     1681 tgcgacaccc aacggtaaca tcccagccac cagaaacaag ctacgccaat caatggaccg
     1741 ataggcttgg tccatattca tggtaccaat gagcaccatc atcacagaac ccgtcagcat
     1801 gatctctgca atgttatcgg agaatacaac tgccagtacc attgtggcga acatgatcaa
     1861 gactgtcagc caattacgcc atgtcatgcg agtagatggg gcagactctg ccaacaagat
     1921 taacccaggt tctgtctgca acaaactcaa agctttttct gtgccctgca tcaaaagccc
     1981 gtcaccaaat tcaagcggta gatccgccag ccgtgtacgg atcgaacgtc cgtgccgcca
     2041 aatggctaac acctttgaat tgaacttctg ctcgaaacga atttcagata acgtttgtga
     2101 agcaagatga gaacgcggcg cgatggcagc ttcgattaat ttcatattgg gtgtggaaag
     2161 ataatcctgc tcccattgcc ccgatggcaa tatattcaac atctccccca gtgactgtgg
     2221 caaactatct tcggggcgag ccacaatgag taaaatatca tcgcctttta ttatggtctt
     2281 tgcctcaatg ggtaatgtgt tgcgtctgcg ctggatggcg atgacattga ggtgatattt
     2341 ttcccgcaaa ccgctttttt ccagtgtcac accatccata cgcccacctt tgcatacttc
     2401 tgcacgtact aaccgttcag caagttgata ggtcttgatc aactcaactg aagcattctc
     2461 ctcttcatgc cgctgagcgg gcgattggtt cggcaataat tttcgtcccc ataaaaccat
     2521 gaaaaggatt cctgcaaatg tcagtgtcag ccccataggc agaaaatctg tcaaagagaa
     2581 cgcaggtaaa ttttcatcgc gcaacagtcc actcaccaca aggttgaccg ttgtcaacaa
     2641 ggttgccata cctcccaaga tcgttccaaa tgccagtggc atcaacaatt tcgatggaga
     2701 aattttcatc ttgtgtgcta taccagatat cgaaggcagc aataaagatg ctgcggcaat
     2761 attattcata aacagtgaca acacagcaga tgcggtcatc acaccaaaga ttaatcttcg
     2821 ctcgcccgcg ccgaacaact taataataaa aagtccaata cgttcggtca tgccagagcg
     2881 gcgcaacccc tccgcaagga tgaacgcgga aagcatggta attacagcct gtcggctaaa
     2941 tcctgagaag gcttcctgcg gagtcaaaat tccgctcaaa cccaaggc
//
LOCUS       DAOQJE010000431         2984 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_22332_length_2984_cov_5.704677, whole genome shotgun sequence.
ACCESSION   DAOQJE010000431 DAOQJE010000000
VERSION     DAOQJE010000431.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2984)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2984)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2984
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22332_length_2984_cov_5.704677"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            439..1866
                     /gene="kaiC"
                     /locus_tag="PLT08_11310"
     CDS             439..1866
                     /gene="kaiC"
                     /locus_tag="PLT08_11310"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF006799.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="circadian clock protein KaiC"
                     /protein_id="HNE05099.1"
                     /translation="MATQTLKTPTKIKGLDEVLNGGVPTGGLTLLNGGPGTGKTLFGL
                     EFLVRGAQEGIPGILITFEEHEEALHHYATTFGWNTAMLEKQGLLSIIGARFDPEGLL
                     SGDFDLGGILAILRHKTEKLSAKQVVIDAPDVFLRLLDNRGKERAELYKLINWLRDEK
                     LTALMTVKADGGDVARSDYEFLDYLADCVIQLDQRVIEQVTTRRVRVVKYRGSAHGRN
                     EYPFAMTDQGIWIIPITETQLNHAALGAPFPTGVAGLDAYMSGGYLRASCNLVTGTSG
                     TGKTTFAASFARTSAANGKRVFYINFEESPDSMVSCMLSPGIDLRPAMEDGSLRFLSV
                     MPEAQGIEEHLIQAFRGIEELKPDIVIVDAISACRRMGSRNSAFDYLLRLIDHCKTIG
                     ITSLLTNLTDSVKETQEITGIDLSSMIDTVVILRNVEYNGQFQRELSVLKARGRQHSN
                     KVHKFQITDHGVSIVNSNIEDAHDR"
     gene            1856..2152
                     /locus_tag="PLT08_11315"
     CDS             1856..2152
                     /locus_tag="PLT08_11315"
                     /inference="COORDINATES: protein motif:HMM:NF019309.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0048511 - rhythmic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="circadian clock KaiB family protein"
                     /protein_id="HNE05100.1"
                     /translation="MTANFKPNVLKKRYTLRLFVAGNATNSRIARENLEQLLARHPEH
                     EFEVEIVDLNVQPEFALDQGVFISPALQILEPSSGGIVYGNLSQKEVLEKVLNL"
     gene            2184..>2984
                     /locus_tag="PLT08_11320"
     CDS             2184..>2984
                     /locus_tag="PLT08_11320"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HNE05101.1"
                     /translation="MIQAKPTYQELEQRCLTAESALAAIRSGQADTVLGEQGVLVLRL
                     KEAEKALQEAELKYRTLAENSPDIIYIIDLTKNKSGYLNRETLLGYTLEELERPGEFL
                     LSKIHPDDLSAVKAHWQAALAGNMNAEMEYRLQLKDGSWEWLQSRPRILSWDVNGRPT
                     QLLVTLSIITERKQAEKRLRSMLDIAQTMSTSLDTKTTLQQIVDSITQVTGLDSGAIY
                     TLTGNQLYLATTTPALPPEMPDQFRFANLADPPHIQITLAKGSSVIIPD"
BASE COUNT          837 a          640 c          709 g          798 t
ORIGIN      
        1 cggtcaaccg tatttggatg ttaatcacaa agccgattat gagttaaaag atttaagtgg
       61 attgctagat attttgaaat aaggatcaat tagctgagtt gaatgactac tccaagtttt
      121 tttgaaagtc tttcatatgg caaaacaaac ctaaattttg aagggaaagg ctgttttttc
      181 cgagaaatgg gaggaaaaca ccctcaaatc ataatgaaaa gcatgtgtaa aaatgcaaaa
      241 atgtgtataa aataaattta gccttttaac gtttgtgcgg ttaggattgc ggggagacga
      301 tgaaatcggt atcgagagat gatcatatta aagtgtaatg tcatatttgc aatgaaatag
      361 ttccatacgt taggcaagca gactagcctt gtttgagaca taaccagatc ctattctctt
      421 gaaaggaatg ctgattttat ggcaacccaa acccttaaaa ctcctaccaa aataaaaggt
      481 ctcgatgaag tccttaatgg aggtgtgccc actggcggac tgaccttgtt gaacggagga
      541 cccggcaccg gcaagacttt gtttgggcta gagttcctgg tgcgaggcgc acaagagggg
      601 attcctggca tcctaattac gtttgaagaa catgaagaag ccttgcatca ttacgccact
      661 acatttggct ggaatactgc catgcttgaa aaacaaggac ttctttcgat cattggggca
      721 aggttcgacc cggaggggtt actctcaggc gattttgact tgggtggcat tctggcgatc
      781 ttgcgccaca aaacagaaaa gctttcagct aagcaggttg ttattgatgc tcccgatgta
      841 tttctccgtt tgcttgataa tcgtggcaag gagcgtgcgg agttatataa actaatcaac
      901 tggttgcgtg acgaaaagtt gactgctctg atgacggtca aagcagatgg gggggatgtt
      961 gcccgttctg attatgagtt tcttgattat cttgcagatt gtgtgatcca acttgatcaa
     1021 cgtgtgatcg aacaagtcac cacccgtcgg gtacgtgtgg tcaagtatcg cggctctgca
     1081 catggacgga atgagtaccc gtttgccatg accgatcaag gcatttggat cattccgatc
     1141 acggaaacac aactaaacca tgcggcgctg ggtgcgccgt tccccaccgg tgtggctggt
     1201 ttggatgcat atatgagcgg cgggtatctg cgtgcttcct gcaatttggt cacaggtaca
     1261 tctggtacgg gtaagacaac ctttgccgct tcgtttgcgc gtacatccgc tgccaatggc
     1321 aaacgcgtct tttacatcaa ctttgaagaa tcaccagact caatggtatc ttgcatgctt
     1381 agccccggta tagatctgcg cccagctatg gaagatggct ctctgaggtt cttatctgtg
     1441 atgcctgagg cgcagggaat cgaagagcat cttattcaag catttcgcgg gattgaggaa
     1501 ttgaaacccg atatcgtgat cgtagatgcc atctcggctt gtcgacgcat ggggtcacgt
     1561 aattctgcct tcgattattt gttgcgtctg atcgatcact gtaagactat tggtattacc
     1621 agcctgttga ccaacctaac cgatagtgta aaagaaactc aggaaatcac tggcattgac
     1681 ctgtcttcca tgattgacac tgtagttatt ttgcgcaatg tagaatacaa cggacaattc
     1741 caacgagaac tgtctgtgct taaagcccga ggacgtcaac attcaaataa agttcataag
     1801 tttcagatta ccgatcatgg ggtttctata gtgaattcaa atatagagga tgcccatgac
     1861 cgctaatttc aaacctaacg ttcttaaaaa acgttatacc ctgcgcttgt ttgttgccgg
     1921 gaatgccacc aattcacgga tcgcgcgtga aaacttagag cagttgttgg caagacaccc
     1981 tgagcatgaa tttgaagttg aaattgttga cctcaacgta caacccgaat ttgccttgga
     2041 tcagggtgtg tttatatccc ccgccttaca aatccttgaa ccaagttctg gtgggattgt
     2101 gtacggcaac ttaagtcaaa aagaagtttt ggaaaaagtc ttgaaccttt gatctggttt
     2161 tgcaaagaaa cggagcgtgc cccttgattc aggcgaaacc gacatatcag gaattggaac
     2221 aacgctgcct aacagctgag tccgcattag cggcgatccg ctcaggacag gctgatactg
     2281 tacttggtga acagggagta ttggtcttgc gcctgaaaga ggcagaaaaa gccttacaag
     2341 aagcagaact taaatatcgc accctggcag aaaattctcc agatattatt tacattattg
     2401 atctaacgaa aaacaagtcg gggtatttga accgcgaaac actgttgggg tataccttgg
     2461 aagaattgga aagaccgggg gagtttctgc tttccaaaat acatcccgac gatctttctg
     2521 ccgtgaaagc gcattggcag gcggcacttg caggcaacat gaatgcagag atggaatacc
     2581 gtttgcagtt gaaagatggt agttgggaat ggttgcagag ccgcccgcgc atcttgagtt
     2641 gggatgtgaa tggtaggccc actcaattat tagttacact tagtataatc actgaacgca
     2701 aacaggcgga aaaacgttta cgttctatgc tagacattgc tcaaacaatg tccacctctc
     2761 ttgatacaaa aacgaccttg caacaaattg ttgacagcat cacacaagtg actgggttag
     2821 atagcggtgc gatctacacc ctgacaggca accaactcta tttagcaact accacgcctg
     2881 ccttgccgcc cgaaatgcca gaccaatttc gtttcgccaa tctggctgac cccccccata
     2941 ttcaaattac acttgcaaaa ggctcttctg tcatcatacc tgat
//
LOCUS       DAOQJE010000432         2981 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_22361_length_2981_cov_6.404990, whole genome shotgun sequence.
ACCESSION   DAOQJE010000432 DAOQJE010000000
VERSION     DAOQJE010000432.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2981)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2981)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2981
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22361_length_2981_cov_6.404990"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..920
                     /locus_tag="PLT08_11325"
     CDS             <1..920
                     /locus_tag="PLT08_11325"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNE05102.1"
                     /translation="FGSSSTEWIKTIRRWSEDPSTYKVGEMYAEQLELENGRIVLIHL
                     APVILIHDFLGTVSIFRDITREVEVDRLKSEFVATVSHELRTPMTAIKGYVDILTMGA
                     AGALNENQMHFLEVVRNNIERLNILVSDLLDISRIESGRVKLEQGKVNLYDIAEEVVA
                     DVLRRSQNESKPIALSLDAPKNLPPIQGDGARVRQIISNLVNNAFNYTPENGAILVNI
                     HDADGEVQVDVQDNGIGVKPEDQDRLFERFYRGEHPLVLATPGTGLGLPIVRQLVEMH
                     NGRIWMKSTGVPGEGSIFSFTLPVYKNGA"
     gene            927..1301
                     /locus_tag="PLT08_11330"
     CDS             927..1301
                     /locus_tag="PLT08_11330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HNE05103.1"
                     /translation="MARILIAEDEPDIRELVAFTLRFAGHEVTTTANGEEAYHKAQEM
                     IPDLIMMDVRMPKMTGYDACRAMKQIPSLKDIPVVFLSAKGQDSEIQTGLDAGAEEYL
                     LKPFAPDQLVERVKAILAKFGK"
     gene            1302..2090
                     /locus_tag="PLT08_11335"
     CDS             1302..2090
                     /locus_tag="PLT08_11335"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HNE05104.1"
                     /translation="MSAIILDGSMVHYEALGRGRPIIFLHGWVGSWRYWINAMQAAST
                     SFRAYAIDLFGFGDTVRSENNYTIEKQALLLERFLNEMGIGKVALVGHDLGALVAFEY
                     LKKHYESVDRMMTIGVPLQYETLATRMRTAPTTELAEWLSARQPDALTALADASKADP
                     LAVTTSMSSLQVDGIFPNVRNFGVPCLFVYGANDPAFTTVPSQETSNLPIHMHQINLE
                     GSGHYPMLDNVSQFNRLLIDFLALDSGASPSELQMKEEWKRRVR"
     regulatory      2162..2271
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            2344..>2981
                     /locus_tag="PLT08_11340"
     CDS             2344..>2981
                     /locus_tag="PLT08_11340"
                     /EC_number="2.7.6.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004788 - thiamine diphosphokinase
                     activity [Evidence IEA];
                     GO_process: GO:0006772 - thiamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine diphosphokinase"
                     /protein_id="HNE05105.1"
                     /translation="MRIIIFANGNLPNLEKAHSLICPDDFILCADGGTRHALALGLTP
                     NVVIGDLDSVTEEERRKMKEMGVEVMEFSMDKNETDLELTINHALTLEPEQILILAAL
                     GGRMDQTLANIALLSDPRLSTFDVRLTDGVEEIFFCRDQAKVEGRSGDIVSLIPWQGE
                     VTGVFTENLRWHLHHETLYPDKTRGISNEMTSDTSTVSITSGLLLITHITGN"
BASE COUNT          778 a          750 c          712 g          741 t
ORIGIN      
        1 tgttcggtag ctcttccacc gaatggatta agaccattcg ccgttggtct gaagacccat
       61 ccacatacaa agtgggtgag atgtatgcag aacaattgga attggaaaac ggacgcattg
      121 tcctcatcca cttggcgccg gtgatcctca tccacgactt cctcggcacc gtgtccatct
      181 tccgcgatat tactcgcgaa gtggaagtgg atcgcctcaa atctgaattt gtggcgacgg
      241 tcagccatga gttgcgcaca cccatgaccg ccatcaaagg ttatgtagat attctcacca
      301 tgggtgcggc gggtgctttg aatgaaaacc agatgcactt cctcgaagtg gtgagaaata
      361 acattgaaag acttaacatt ctcgtgagcg atttgcttga tatttcccgc atcgaatctg
      421 gacgtgtgaa acttgagcaa ggcaaggtca atctgtatga tattgctgaa gaagtggtgg
      481 cagatgtttt acgccgctca caaaatgaaa gcaagccgat tgcactttca ctagatgcgc
      541 cgaagaacct tccaccgatt caaggcgatg gtgcaagagt ccgccagatc attagcaacc
      601 ttgtaaacaa tgcattcaat tacacccccg aaaatggcgc cattctggtg aatatccatg
      661 atgctgatgg cgaagtgcaa gtagacgtgc aggataacgg tattggcgta aagcctgaag
      721 accaagaccg cctctttgaa cgtttttacc gtggcgagca tccacttgtg ttggcaacgc
      781 cgggtactgg tttaggtttg cccatcgttc gtcagttggt cgaaatgcac aatggacgta
      841 tttggatgaa aagcacaggc gtccctggtg aaggaagtat tttctctttc accttgcctg
      901 tctataaaaa cggagcataa atttcaatgg caagaatttt gatcgcagaa gatgaaccgg
      961 atattcgtga gttggtggcg tttacattac gctttgcggg tcatgaagtg accaccactg
     1021 ccaacggcga agaagcctat cacaaagcac aggagatgat tccagacctc atcatgatgg
     1081 atgttcgtat gccgaaaatg acgggctatg atgcctgccg tgccatgaag cagattccat
     1141 ctttgaaaga tattcctgtg gtatttctct ctgcaaaagg acaggactct gaaattcaaa
     1201 ccgggcttga tgcaggagcc gaagaatacc tgcttaaacc ttttgcccca gaccagcttg
     1261 tggaacgggt gaaagcgatc cttgcgaaat ttggaaaata aatgagcgca attattcttg
     1321 atggttcgat ggtgcattat gaggcgttgg gacgtggtcg tcccatcatc ttcctgcacg
     1381 gctgggttgg ctcatggcga tattggatca atgccatgca agccgcatcc acatcgttcc
     1441 gcgcctatgc catcgatctc tttggttttg gcgataccgt tcgcagtgag aataattaca
     1501 ccattgaaaa acaagctctg ttgcttgaac gcttcttgaa tgaaatgggt attgggaagg
     1561 tcgctttggt agggcatgac cttggcgcgc tcgtcgcttt tgaatacctt aaaaagcact
     1621 acgagagtgt agaccgtatg atgacaatcg gcgtaccgtt gcaatacgaa acacttgcca
     1681 cacgcatgcg gactgccccc acaaccgaac ttgcggaatg gctgtctgcg cgccagcctg
     1741 atgccttgac cgctctcgca gacgcctcca aagcggaccc gttggctgtg accacctcga
     1801 tgagcagttt acaagttgac ggtattttcc cgaacgttcg taattttggt gtgccatgtc
     1861 tttttgtata tggtgccaat gacccagcct tcacgaccgt cccttcacaa gagacaagta
     1921 atctaccaat tcatatgcac cagattaact tggaaggatc tgggcattac cccatgcttg
     1981 ataacgtctc gcaattcaac cgcctcctga ttgatttcct cgcactcgac tcaggcgcca
     2041 gcccaagtga actgcaaatg aaagaggaat ggaaacgccg cgttcgctaa aaataccgtg
     2101 agaaaaaatc tcgcggtatt tttttacttg acattcccac gtcacagtcc tatactccaa
     2161 acacaaccga acggagagcc tttacgaaag gctgagagga gcgtgaacgc gctcgatccg
     2221 catcacctga tctggttaat accagcggag ggatttctca atacacctgc caacatcctc
     2281 cgctgctgtg aggatgtttt tgttttaacc tttgtgtacc ttcgtgaccc tttgtggtga
     2341 aaaatgagaa tcatcatctt tgccaacggc aacctgccaa acctcgaaaa agcccactcc
     2401 ctcatctgcc ccgatgactt cattctttgc gcggacggtg ggacacgtca tgcccttgcg
     2461 ttgggactca ctcctaatgt ggtgattgga gatctggatt cggttacgga ggaagaaaga
     2521 agaaagatga aagaaatggg cgttgaagtt atggaatttt cgatggataa gaacgaaaca
     2581 gacctcgaac tcaccatcaa ccatgcactg actcttgagc ctgaacaaat cctaatcctc
     2641 gccgccctcg gcggtcgcat ggaccaaacc ttagccaaca tcgctctcct ctctgaccct
     2701 cgactttcga ccttcgacgt tcgactcacc gacggcgtag aagaaatttt cttttgcaga
     2761 gaccaagcca aagttgaagg aagaagcgga gacatcgtct cgctcatccc ctggcagggc
     2821 gaagtcaccg gcgtcttcac cgaaaacctg cgctggcatc ttcatcacga aacgctttac
     2881 cccgataaaa cccgcggtat cagcaacgaa atgacttctg atacatccac ggtttcaatt
     2941 acgagcggat tgttgctcat cactcacatc actggtaact g
//
LOCUS       DAOQJE010000433         2980 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_22373_length_2980_cov_5.788034, whole genome shotgun sequence.
ACCESSION   DAOQJE010000433 DAOQJE010000000
VERSION     DAOQJE010000433.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2980)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2980)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2980
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22373_length_2980_cov_5.788034"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..511
                     /locus_tag="PLT08_11345"
     CDS             <1..511
                     /locus_tag="PLT08_11345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE05106.1"
                     /translation="IGSPGNWQGMDKLVPFARKYSDLTINIIGDSPQDFSEPIPDNLH
                     IHGFLDKDAIRAIFSNTDAVFSQLGLHRKNMKENPTLKVREALAYGLPLILAHYDADL
                     NDLDTDCILRIPNTEDNLTSHGDAVHEFAYHVMGKRIERELIASRIDQRKKEEKRLAF
                     FEQVLAGKK"
     gene            511..1662
                     /locus_tag="PLT08_11350"
     CDS             511..1662
                     /locus_tag="PLT08_11350"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HNE05107.1"
                     /translation="MPYSIVLLGTQMAVGGAQKLLLEQALWFQQHGNRVTVLFFYDRD
                     NLHEKWTKTYPFEIQNLEAFDKKAGGLRSLPKLLGGLLKLWQVLKRGKFDAIITFTHD
                     SNLLGMPLAWLAGIHARVGTHLGEIRGMSKWRDGLHTFLVNHGVIQTLVASSARTRNN
                     AIEVGVHPERITTIYNAIMPFDVSSINRESVREKLGLKKDEIFFLAVGRLVYEKGHEF
                     FIEAMAKVVKDNTRAIAGICGAGPLQGELQSQIEKLNIQANMKLLGQWDSIPEILAAA
                     DVFVLPSRWEGLPMALLEGMMAGLPVIATRVEGVDEVVQPGEHGLLVPLESPAELAQA
                     ILQLLRSPQDRQRMGAAARERVLSSYTTDRMCEAYLQVIEQGLGVGKAG"
     gene            1687..>2980
                     /locus_tag="PLT08_11355"
     CDS             1687..>2980
                     /locus_tag="PLT08_11355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE05108.1"
                     /translation="MKRSSIDFGKLIAITIAAWILVEACVEFYNVAWGTGEAVGLFSP
                     RWLILFVSFVLSCVFVYVCVGIAVLDRERFFALIQNIVTLRNRIPILSWALAILFLTF
                     PAWFLQRTMWGVVFDGIYFRLFLWVLTLFLVSFLITRGTTLIEWKSFLVVLILTSSVF
                     TIAISLQGVTDYPFSMGWSEGNRIWDYSMRFGRDRYGIADNQDIYVLLDSGRMLIGGL
                     PFLIPNVTIEIVRLWIGLTLILPYFLVGIAAYIVAEKNVRIWILMTLWVFLFLRQGPI
                     HSPLVLAAALTIFTWRKPLWLAIPLIIYAGYFAQASRFTWLFAPGLWIGMLELAGASL
                     SNGKLNRAHWVRTITLGLSGAFGGFLLPKLLPLINIASVATTPVDVTEQIANVGVNSE
                     FVTYAVTDQPLLWYRLLPNSTYGTGILLGLVIAVLPIIL"
BASE COUNT          733 a          588 c          726 g          933 t
ORIGIN      
        1 tattggctcg ccgggcaact ggcaagggat ggataaacta gttccatttg cacgtaaata
       61 ttcggatttg acgattaata ttattggaga ctctccacag gatttttctg aacccatccc
      121 tgataatctg catattcacg gttttcttga taaagatgca attcgtgcaa tattttcaaa
      181 tactgatgcg gtgttcagtc aacttggctt gcatcgaaaa aatatgaagg aaaacccgac
      241 gctaaaagtg cgtgaggcat tagcctacgg acttccattg atacttgctc attacgatgc
      301 tgatctgaat gacttggata cggattgcat cttaagaatt ccaaacacag aagataatct
      361 gacgtctcat ggggatgctg tgcatgaatt tgcatatcat gtaatgggta aaaggattga
      421 acgtgagtta attgcctcaa gaattgacca gcgcaagaaa gaagaaaaac gtcttgcatt
      481 ctttgaacaa gtcctcgcgg gaaagaaata atgccatatt ccatcgtctt attaggcacc
      541 caaatggcgg ttggcggcgc tcagaaactt ttactggaac aggcgctttg gtttcaacag
      601 catggaaata gagtgaccgt cctctttttc tatgatcgcg acaacttgca cgaaaagtgg
      661 acaaagacct atccgtttga aattcaaaat ctggaagctt ttgataaaaa agctggcgga
      721 cttcgcagcc tgcctaaatt gttgggtgga ttgttgaaac tgtggcaagt tctcaaacgt
      781 gggaaatttg atgccatcat tacctttacg catgatagca atctgttagg tatgccactg
      841 gcgtggctgg cagggattca tgcccgtgta ggcacacacc ttggcgaaat tcgtggcatg
      901 tcgaagtggc gtgatggatt gcataccttt cttgtcaatc acggagtgat tcaaacactt
      961 gtggcatctt cagcgcggac ccgcaataat gccattgaag ttggtgtaca tcctgaaaga
     1021 atcactacta tttacaatgc catcatgcct tttgatgtgt caagtattaa tcgtgaatct
     1081 gtacgcgaaa agctgggatt gaaaaaggat gaaatctttt ttctagctgt cgggcggctt
     1141 gtctatgaaa aggggcatga gttctttatt gaagctatgg ctaaagtggt gaaagacaat
     1201 actcgtgcta ttgctggcat ttgtggagca ggtccgttgc agggagaatt gcagtctcaa
     1261 atcgagaagt taaatattca agcgaatatg aaactgctcg ggcaatggga ctccattcca
     1321 gaaatccttg cagccgcaga tgtttttgtt ttgccatcac gttgggaggg gctcccaatg
     1381 gcattgcttg agggcatgat ggcagggctg cctgtcattg ctacacgagt ggaaggtgta
     1441 gatgaagttg tccagccagg tgaacatgga ctattagtgc cgcttgagtc tccagcagaa
     1501 ctggcgcagg ctatattaca attgctccgc tcacctcaag accgtcagcg catgggagcg
     1561 gcggcgagag agagagtatt aagctcttac actaccgatc gcatgtgtga agcgtatttg
     1621 caggtgatcg aacaaggctt gggtgtggga aaagccgggt aagcgtaatc aagcagaggc
     1681 ttaaggatga agcgttcgtc tatcgatttt ggaaaactaa ttgcaattac aatcgctgca
     1741 tggatactcg ttgaggcatg tgtcgagttc tataatgtgg cgtggggcac gggcgaggcg
     1801 gtggggttat tctcaccacg ttggcttatc ctttttgttt cttttgtttt atcatgtgtt
     1861 tttgtatatg tatgtgtggg cattgctgtt cttgatcgcg aaagattttt cgctttaatt
     1921 caaaatatag ttacattacg aaatagaata ccaatcttaa gctgggctct tgccatttta
     1981 tttttgacct tcccggcatg gtttttgcaa cgaaccatgt ggggtgttgt ttttgatggc
     2041 atttattttc gtttgttttt atgggtattg acgctttttc ttgtatcttt tcttattact
     2101 cgcggaacca cattgattga atggaagtca ttcctcgtcg tattgatact gacctcaagt
     2161 gtttttacaa tcgcgatttc tttgcaaggt gtaacagatt acccattctc tatgggttgg
     2221 tctgaaggta atcgtatttg ggattattca atgcgcttcg ggcgtgatcg ttatggcatt
     2281 gctgataacc aggatattta tgttctgctt gattctgggc gtatgctaat aggtggtttg
     2341 ccatttttaa ttcccaatgt gactattgaa atagtgcgcc tatggattgg gcttacactg
     2401 atcctgccgt attttctggt tggaattgcc gcttatattg ttgctgaaaa aaatgttcgt
     2461 atttggatat tgatgacttt atgggtcttc ctgtttttgc gacagggtcc tattcattct
     2521 ccgttagttc ttgctgcggc attgactatc ttcacctggc gtaaaccatt atggcttgca
     2581 attccattga ttatctatgc tggatatttt gcccaagcga gtcgatttac ctggttgttc
     2641 gcacctggtc tatggatcgg aatgcttgaa ttagcaggcg cttctttatc taacggaaaa
     2701 ctgaatcgag cccattgggt tcgcactatc actttaggct tgtccggcgc atttggcgga
     2761 ttccttttgc ccaaactgtt gccattgatt aatattgcga gcgttgccac aactcctgtg
     2821 gatgtcaccg aacagattgc caatgtcggg gtcaactcag aattcgtgac ctatgcagtg
     2881 accgaccagc ccctcttgtg gtatcgccta cttcccaact caacatatgg aactggtatt
     2941 ttgcttgggc tggtcattgc cgtattgccg attattcttt
//
LOCUS       DAOQJE010000434         2978 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_22392_length_2978_cov_5.994184, whole genome shotgun sequence.
ACCESSION   DAOQJE010000434 DAOQJE010000000
VERSION     DAOQJE010000434.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2978)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2978)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2978
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22392_length_2978_cov_5.994184"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(23..274)
                     /gene="minE"
                     /locus_tag="PLT08_11360"
     CDS             complement(23..274)
                     /gene="minE"
                     /locus_tag="PLT08_11360"
                     /inference="COORDINATES: protein motif:HMM:NF001422.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA];
                     GO_process: GO:0000918 - division septum site selection
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division topological specificity factor
                     MinE"
                     /protein_id="HNE05109.1"
                     /translation="MDFFTRKRSATSAKERLQLVLVHDRTDLTPGQLESLKDELLKAI
                     SKYIEIDPEAVRIELEKDGREQRLVADIPLKGATRHRAG"
     gene            complement(276..1073)
                     /gene="minD"
                     /locus_tag="PLT08_11365"
     CDS             complement(276..1073)
                     /gene="minD"
                     /locus_tag="PLT08_11365"
                     /inference="COORDINATES: protein motif:HMM:TIGR01968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0000918 - division septum site selection
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinD"
                     /protein_id="HNE05110.1"
                     /translation="MTAQVITVTSGKGGVGKTTAVANLATALAMDGKRVVCIDGDIGL
                     RNLDVVMGLENRIVYDIVDVIEGRCKLKQAMIRDKHYQDMYLIPAAQTRDKNAVSPSD
                     MIRICNDLRPDTDFIVIDSPAGIERGFRNAIAAADSVLVVTNPEVSAVRDADRVVGIL
                     EAEEKGNPALIINRLNPALVKNNDMLSAEDVLDLLGIRLIGIVPEDENVIIGSNRGTP
                     IAADGKSRAGQAFRNIAKRLQGVDVPFMDLTPQSGLWNTIQRLAGRK"
     gene            complement(1075..1773)
                     /gene="minC"
                     /locus_tag="PLT08_11370"
     CDS             complement(1075..1773)
                     /gene="minC"
                     /locus_tag="PLT08_11370"
                     /inference="COORDINATES: protein motif:HMM:TIGR01222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004857 - enzyme inhibitor activity
                     [Evidence IEA];
                     GO_process: GO:0000918 - division septum site selection
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinC"
                     /protein_id="HNE05111.1"
                     /translation="MEPTTSLVQIKGIRDGLLATFTEAAWEEQRNALMEQINERVSFF
                     QGARLAMDVGTQVLKVNDLVDLRDWLSERNITLWAIVSESPTTETTAQLLGLATRISK
                     PRPEETRHVHEAVSSDTALFVSKTVRSGTRIEYPGTIVVFGDVNPGAEIVAEGNVIVW
                     GRVRGVIHAGVKGDRSAFICALDLSANQLRIANESSAMLKPQKDPKPEIASINSEGKL
                     QAELWQTGETLIRK"
     gene            complement(1827..>2978)
                     /locus_tag="PLT08_11375"
     CDS             complement(1827..>2978)
                     /locus_tag="PLT08_11375"
                     /inference="COORDINATES: protein motif:HMM:NF013100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HNE05112.1"
                     /translation="LLNNAISAEYPPGSTFKLSTATGAFNEGIVDLDTIVNAPGRLLL
                     CERFNPNDPCTDNNTRPFVDHIFDQRPEGFGPITFTQCIAYSSNVCFYKLGGGFEDEI
                     PQGLGIERLQQYARALGYDNPSGIELPGEQNGLIPDPDWKRINTGENWSTGDTYIASV
                     GQGFVLATPLQVLMSGATIANHGKLMQPTIVRQVTDGDGNVVPMWFDPDPEDFKAAPF
                     QTENSYQISPFTPNMKWDITVDPLIQGYSCEGPYCTLTDDFKVVKPETIEAVRAGTRR
                     AVTDTSFGTLYRLLKDFPIAVAGKTGTAEYCDDVARKADRCNFGAWPTHSWTIAYAPY
                     EDPEIAIIAFAYNGGEGASVAGPMVARMIKAYFEIKALDIAQGVNVPAP"
BASE COUNT          770 a          760 c          665 g          783 t
ORIGIN      
        1 ccatgtccta ttcaaaaaca tatcaacctg cgcgatgacg cgttgcgccc tttaatggaa
       61 tatctgccac caaacgttgc tcgcgtccat ctttttccaa ttcaattcga acagcttcag
      121 ggtcaatctc aatatacttg gagattgcct tcaagagttc atccttcaaa gattctaatt
      181 gcccaggggt caggtccgta cggtcgtgaa ccaacaccaa ctgtaatcgt tcttttgcgc
      241 tcgtagcgct ccgcttgcgt gtgaagaagt ccatatcact tccttccagc caaacgttga
      301 atggtattcc acaagccact ctgcggagta aggtccataa atgggacatc aactccttgc
      361 aagcgtttgg caatattacg gaaagcctgc cccgcacggc tctttccatc ggctgcaatc
      421 ggagtcccgc ggttcgaacc gatgattaca ttttcatctt ctggcacaat accaatcaaa
      481 cgaatgccta aaagatccaa cacatcttcc gcagaaagca tgtcgttgtt tttcacaaga
      541 gcaggattca gacgattgat gataagcgcc ggattgcctt tttcttccgc ttcaagaatg
      601 cctaccactc ggtctgcatc gcgcaccgca ctgacttcgg ggttggtcac tacgaggacg
      661 ctgtctgctg cggcgatggc gtttcgaaat ccgcgctcga tacctgcagg ggagtcaatg
      721 acaatgaaat cagtatccgg tctgaggtca ttgcagatgc ggatcatgtc agatggcgag
      781 acagcatttt tgtcacgtgt ttgagccgcc ggaatcaggt acatatcctg ataatgctta
      841 tcgcgaatca ttgcctgttt gagtttgcaa cgtccttcga tgacatccac aatatcgtag
      901 acgatacgat tctcaagccc catcacaaca tcaaggttac gcaacccaat atcaccgtca
      961 atacaaacga cccgtttgcc atccatggca agagccgtag caagattggc aactgcagtg
     1021 gtctttccca cgccgccttt tccagaagtt acagtgatca cttgagctgt catattattt
     1081 cctaattaaa gtttcgccgg tctgccataa ttctgcttgc aatttacctt cgctgttaat
     1141 gctggcaatc tcgggctttg ggtctttctg tggtttcaac attgcggatg actcatttgc
     1201 aatccgtaat tggtttgcag ataggtctag ggcacaaata aaagcggagc gatctccttt
     1261 tacgccagca tggatcaccc cacgcacacg cccccaaacg atcacatttc cttctgccac
     1321 gatctctgcg ccggggttca catctccaaa gacgacaatc gtacccggat attctatccg
     1381 ggtgccagaa cgaactgttt ttgaaacgaa aagtgcagtg tcgcttgata ctgcctcatg
     1441 cacatgacgg gtttcttcag ggcgtggctt ggagatacgt gttgccaacc caagtagctg
     1501 ggctgttgtt tctgtggtcg gcgattcact gacgattgcc cacaaggtga tatttcgttc
     1561 tgaaagccaa tcgcgaaggt caacaaggtc attgaccttc aatacctgag tcccaacatc
     1621 catggcaagg cgcgctcctt gaaaaaagga aacacgttca ttaatttgct ccatcagagc
     1681 attgcgttgt tcttcccagg cagcctcggt aaaggtcgcg agcagaccat ctcgaatgcc
     1741 tttgatttgg accagggatg ttgtgggttc catagacatg ttcatttagg taacaggcat
     1801 taattatacc tgatgaactt accacgctat ggcgctggta cgtttacacc ctgagcaatg
     1861 tctaatgcct tgatttcaaa atacgcctta atcatacgag cgaccatagg acccgccaca
     1921 ctggcacctt caccgccatt ataggcaaaa gcaataatag cgatctccgg gtcttcatat
     1981 ggagcgtatg caatcgtcca tgagtgagtg ggccaggcac caaaattaca acggtctgct
     2041 ttgcgggcaa catcatcaca gtattcagct gttcccgtct ttcctgctac agcaatagga
     2101 aaatccttaa gtaacctgta aagggtacca aatgaagtgt ccgtcactgc acgccgcgtc
     2161 cctgctcgaa ccgcttcgat ggtttcaggt ttgactactt taaaatcatc ggtcaatgta
     2221 cagtacggac cttcacagga atatccttga atgagaggat cgacagtgat gtcccatttc
     2281 atattaggcg tgaaggggga aatctgataa ctgttctctg tttggaaagg ggcagcctta
     2341 aaatcttcgg ggtctgggtc gaaccacata gggacgacat tcccgtctcc gtctgttact
     2401 tgccggacaa tggtaggctg cattaacttt ccatgattgg caatagtggc gccagacatt
     2461 aaaacttgaa gtggcgttgc caacacaaag ccttgaccta cactcgcaat ataggtatcg
     2521 cccgttgacc agttctcgcc tgtattaata cgcttccaat ctgggtccgg aatcaaaccg
     2581 ttttgttcac cgggcaactc aattcccgaa gggttatcgt acccaagagc tcgagcatat
     2641 tgttgcaacc gctcaattcc taacccttgt ggaatttcat cttcaaaacc accgcccaac
     2701 ttgtaaaaac atacattact ggaatacgca atacattgtg taaaggtaat aggaccaaag
     2761 ccctcaggac gttggtcaaa aatatggtct acaaacgggc gggtgttgtt atctgtacag
     2821 gggtcgttag gattaaatcg ttcgcagagt aatagacgtc ccggagcatt gacgatggta
     2881 tccaaatcga caataccttc attaaaagca ccagttgccg ttgaaagctt gaaggtcgaa
     2941 ccaggagggt attcagccga aattgcatta ttcagcaa
//
LOCUS       DAOQJE010000435         2978 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_22396_length_2978_cov_4.217243, whole genome shotgun sequence.
ACCESSION   DAOQJE010000435 DAOQJE010000000
VERSION     DAOQJE010000435.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2978)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2978)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2978
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22396_length_2978_cov_4.217243"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            47..526
                     /locus_tag="PLT08_11380"
     CDS             47..526
                     /locus_tag="PLT08_11380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE05113.1"
                     /translation="MKRLLLPALIILTFVITACSATLSPLPTQPPSDPVSTVSASPEF
                     DSATRMDDQGAIIFEVTPLNLDSATTESVEFNIVLTTHSIDLSMDLAATATLLTDTGV
                     SVNSTLWDAPRGGHHVEGKLIFPAAQDGKSILDGATKLTLTITNVDAPTRIFEWELK"
     gene            539..1036
                     /locus_tag="PLT08_11385"
     CDS             539..1036
                     /locus_tag="PLT08_11385"
                     /inference="COORDINATES: protein motif:HMM:NF012575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinol-cytochrome c reductase iron-sulfur
                     subunit"
                     /protein_id="HNE05114.1"
                     /translation="MAHKTDISRRDFIKSVAGFVGMLIGIGIGLPSILYLLSPSLEKT
                     QDDLIIDLGSLERYPIGIPTRFEFTRTRVNGWERTATNYGLFVLRKSESEVRVFSNIC
                     THLGCRVTWHPDQGHYISPCHDGHFDMVGNVVSGPPPRPLDEFTAEIKNGNLIVSLPP
                     VKRKG"
     gene            1049..1282
                     /locus_tag="PLT08_11390"
     CDS             1049..1282
                     /locus_tag="PLT08_11390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE05115.1"
                     /translation="MNKRHIWIMLLGCLLPILGLAAVLLLKVPINTVVYVGLALLCPL
                     AHFFMMGNMHNHNSQPDQHHDHSVHDKTSTHSP"
     gene            1254..2348
                     /locus_tag="PLT08_11395"
     CDS             1254..2348
                     /locus_tag="PLT08_11395"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015679.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_function: GO:0071972 - peptidoglycan L,D-transpeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HNE05116.1"
                     /translation="MTRHPLTRRDFLKIAALGAGALAFRPFTWQGISELQQSELLGRI
                     TVGKVDVYARPDGNEQIVGALYEDQVIPWIREVVGTMPGRINQRWVETPYGYVWGGNL
                     QPVRNLPNSPVSTLPSTSLGPGMWVEVTVPYVDLILDNPPSRAPWLQYRESIGLPARF
                     VYSQVVWVDQIRAEADGRVWYRLNERFGSGDLFWGPAEAFRPLTADEIAPVNPEAEEK
                     RIVVNIDYQTLSCYEGKNEVYFARISSGALYDAWGNPVDAWATPVGEFPIWRKAISLP
                     LSGGSASAGWSLPAVGWVSLFVGSGVAIHSTYWHNNYGEPSSRGCVNASPEDAKWIFR
                     WSQPLVSYDPGDVTVEMPGGTRVSVEKKEF"
     gene            complement(2413..>2978)
                     /locus_tag="PLT08_11400"
     CDS             complement(2413..>2978)
                     /locus_tag="PLT08_11400"
                     /inference="COORDINATES: protein motif:HMM:NF017206.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="NmrA family NAD(P)-binding protein"
                     /protein_id="HNE05117.1"
                     /translation="HAVFLSLIGIESTKYVPHYKVETYLKAINMQTTFLRCSFFMQNL
                     NTTHRREIKERNEIFVPVGKAKTAFVDARDIGAVAAVALLEDGHAGRNYDLTGSERLD
                     YWEAARIMSEVLGRRITYRNPNPLYFLIETIRRGTPFMFALVMTGLYTSTRFGMAKPI
                     TDEVERLTGISPITFRQYVTDYEDAWK"
BASE COUNT          685 a          763 c          768 g          762 t
ORIGIN      
        1 tttatcgtga acgacaaaaa cttcaacctg tacttctgga gacaaaatga aacgactctt
       61 acttcccgcc cttattattt tgacttttgt tatcacagcc tgttcagcga ctttatcccc
      121 gcttccaacc cagccgccat ctgatcctgt ttcgacggta tcagcctccc ccgaatttga
      181 ctctgccacc cgcatggacg accagggagc gattatcttt gaagtcaccc cacttaactt
      241 ggactcagcg actaccgaat ctgttgagtt caatattgtc ctgaccaccc attcgattga
      301 cctgagcatg gatctagccg caactgcaac gctcttaacc gatacaggag tcagcgtgaa
      361 ttcaaccctc tgggatgcgc cgcgcggtgg tcatcatgtg gaaggaaaat tgatttttcc
      421 agcggcgcag gatggtaaat ccattctgga tggagcgaca aagctcaccc tcaccattac
      481 caacgtggat gcccccaccc gcatctttga atgggagttg aaatagcaag aggaagaaat
      541 ggcgcacaaa acagatatca gccgtcgtga cttcatcaaa tcggttgccg ggttcgtcgg
      601 aatgctcatc ggcattggga tcggtcttcc ctccattttg tatcttcttt cgccatcgct
      661 tgaaaaaacg caagacgatt taatcatcga tcttggctcg cttgaaagat atccgattgg
      721 cattccaacc cggtttgaat ttacccgcac cagggtcaac ggatgggaac ggacggcaac
      781 caactatggc ttgttcgttc tgcgtaagag tgaaagtgaa gtaagggtat tctctaacat
      841 ttgcacccac cttggctgtc gtgttacctg gcatcctgac cagggacatt acatcagtcc
      901 ctgccatgac ggtcattttg atatggttgg taatgtggtc agcggtccgc cgccgcgtcc
      961 tctggatgaa ttcactgctg aaataaaaaa tggcaatctc attgtttccc ttccgcctgt
     1021 aaagcggaaa gggtaaagga aggttcacat gaataaacgt catatctgga tcatgctgct
     1081 tggctgcttg cttcccattc tgggtctggc agctgtcctg ctcctgaaag tccccatcaa
     1141 tacagtcgtc tatgtggggc tggcattgct ttgcccgctg gctcattttt tcatgatggg
     1201 caacatgcac aatcacaaca gccagccgga ccagcatcac gatcattccg tccatgacaa
     1261 gacatccact cactcgccgt gattttctaa agatcgccgc attgggcgcc ggcgcacttg
     1321 cctttcgacc atttacctgg caggggattt cagaattaca acagtcggaa ctgttggggc
     1381 gtatcacggt tggcaaggtg gatgtatatg cgcgcccaga tgggaatgaa cagatcgtgg
     1441 gcgctctgta tgaggatcag gttatcccct ggattcggga agtggttgga accatgcccg
     1501 gcaggatcaa tcaacgctgg gtcgagactc cctatggtta tgtgtggggc ggaaacctgc
     1561 aacccgtgcg caacctgcca aattcgccag tgtccactct gccaagtacg agtctgggac
     1621 ctggcatgtg ggttgaagtc accgttcctt atgtagactt gattctcgat aatccgccat
     1681 cgcgcgcacc gtggctccaa taccgcgaat cgattgggct gccggcgcgg ttcgtctata
     1741 gtcaggtggt ctgggtggat cagattcgcg ccgaagcgga tggaagagtc tggtatcggt
     1801 tgaatgaacg ctttggatcc ggcgacctgt tctggggtcc ggctgaggcg tttcgtccac
     1861 tgactgcgga tgaaatcgct cccgttaacc cggaagcgga agaaaaaagg atcgtggtca
     1921 atattgatta tcaaacctta tcgtgctatg agggaaagaa tgaggtgtat ttcgcgcgta
     1981 tttccagtgg tgcactgtac gatgcatggg gcaatcctgt ggatgcatgg gcgaccccgg
     2041 taggcgaatt cccgatctgg cggaaggcga tctccttgcc attaagcggt ggaagtgcat
     2101 cagcaggctg gagtctgccg gctgttgggt gggtcagttt attcgttggc tccggtgtgg
     2161 caatccattc cacctattgg cacaacaatt atggtgaacc ctcctcacga ggatgcgtca
     2221 acgccagccc ggaggatgca aaatggatct ttcgctggtc tcaaccactt gtatcttacg
     2281 accctgggga tgttaccgtg gagatgccag gtggaacccg ggtgagcgtt gaaaagaaag
     2341 agttttagcc tatagcatga agggataagt aggggcgttc atgctgaatt aatttgactc
     2401 ggtaatctga gattacttcc aggcatcttc ataatctgtt acatattgcc tgaatgtgat
     2461 cgggctaatc ccagttaatc gctcgacttc atctgtgatc ggtttcgcca ttccgaaacg
     2521 tgtggatgtg tacaaacccg tcatcaccag ggcgaacatg aaaggcgtac ccctgcgtat
     2581 cgtctcgatc aggaaataaa gcgggttcgg gttgcggtag gtgattcttc gtcccaatac
     2641 ctcgctcatg atcctggctg cttcccaata atccaagcgt tcactgccag tcaggtcgta
     2701 gttcctgccg gcatgtccat cctccagcaa agccaccgcc gccaccgccc cgatgtcgcg
     2761 cgcatcgaca aaggcggttt ttgcctttcc caccggcaca aaaatctcat ttctttcctt
     2821 gatctcacgg cgatgggtgg tattcagatt ctgcataaag aaactacagc gtaaaaaggt
     2881 tgtttgcatg ttgattgcct tcaaataggt ctccaccttg tagtggggga catattttgt
     2941 gctctcgatc ccgatcaggg agagaaacac ggcatggg
//
LOCUS       DAOQJE010000436         2976 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_22427_length_2976_cov_5.558028, whole genome shotgun sequence.
ACCESSION   DAOQJE010000436 DAOQJE010000000
VERSION     DAOQJE010000436.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2976)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2976)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2976
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22427_length_2976_cov_5.558028"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..443
                     /locus_tag="PLT08_11405"
     CDS             <1..443
                     /locus_tag="PLT08_11405"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HNE05118.1"
                     /translation="TAIFVRRGDEYLLLKGAPTKRLWAGKYNGLGGHVDRGEDVLSSA
                     RRELMEEAGISADLWLAGTVIVDAGEIGILLFVVVGENPQGEVKGSKEGAPEWVQKEA
                     VSQLPVVEDLPVLLERIHSMKRSSPPFAARSFYENDKLNVVFGE"
     gene            complement(473..1258)
                     /locus_tag="PLT08_11410"
     CDS             complement(473..1258)
                     /locus_tag="PLT08_11410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE05119.1"
                     /translation="MRQTHQLVITILVLVLLTACGGNEQSSNISLPTVLPPAGQTTSD
                     IVSQLCDNPLFPVKQNASWSYFSSGGPNGDFAYTDTITSLTPDGFVLTSQFPALSLTQ
                     NWICAPEGYIAQQLGGGTTASVSMQNMITDFKTIQVSGVSLPRTLTPGMQWKYDLSMQ
                     GAVAMPGEQTQSPGTFALTMQELGTETITVPAGTFDATKIQATFTANIDVDFEGSIIR
                     YTVNGTSLLWYAPGVGFAKSIENIDFSGTSFTSTTELQSYNIP"
     gene            complement(1264..2430)
                     /locus_tag="PLT08_11415"
     CDS             complement(1264..2430)
                     /locus_tag="PLT08_11415"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNE05120.1"
                     /translation="MTTLRTPARIIPRAFPGIKPEEVQEIIVNSQIKSYPAGTILCQE
                     DAIEHTFYMILEGDFEVTKTINNKEKRLLKTLIAGDFFGEMALIHNAPRAATVQSLTP
                     AVVLELDKQGFDRVLKHSPSVATAMVAEISNRLRANDQLAIEDLRLRAREIADAYQKL
                     AEQDMARNEFLTSIAHELRTPLMVASGYLHTLKKGVLTGEQLQSTIEIVSRNVDQIVN
                     LTNDLLFLQEQDLVLRDFQALNLYHVVEGVVEKYQAKAKTRSINIKVRGSANIPSVQG
                     DEPSLERAIAGLVDNAIKFSPYGGTVEIRFADLGNYVSVSVVDYGIGIDPQIRPRIFD
                     RFFHLEKSNDEIYDGLGIGLSITRQVIQQHRGMLDVDSQPGKGSTFTISLPKWK"
     gene            complement(2497..2727)
                     /locus_tag="PLT08_11420"
     CDS             complement(2497..2727)
                     /locus_tag="PLT08_11420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE05121.1"
                     /translation="MNDLEKIIMEEVSTLDDMRLIDVIGFIRYLKAEKPIRQEWIAGW
                     YESAIKSIHAREEELHITPAELEKQLRKKQSE"
BASE COUNT          736 a          640 c          744 g          856 t
ORIGIN      
        1 gcacggcgat tttcgtccgg cgcggggatg agtatttatt gctcaaaggt gcgcccacaa
       61 aacgattgtg ggctggcaaa tataatggct taggtgggca tgtagatcga ggagaggatg
      121 tactttcttc ggcaagacgc gagttgatgg aagaagctgg aatttctgcc gacctttggc
      181 tggctgggac agtgattgtc gatgctggag aaattggtat tttgcttttt gttgttgtag
      241 gggagaaccc tcaaggcgaa gtgaaaggtt caaaagaagg cgcaccagaa tgggtccaga
      301 aagaggcggt gagtcaactt cccgtcgtgg aggatctgcc ggtcttgctt gagcgcatcc
      361 attcaatgaa aagaagttcc ccgccttttg cggcgcggtc tttttatgag aacgataaat
      421 tgaacgtggt ctttggagaa tgaaaacgcc gcctaataag gcggcgtttg ttttacggaa
      481 tgttataact ttgcagctcg gtggtggatg taaatgatgt gccgctgaaa tcgatgtttt
      541 caatggattt tgcgaagccc acgcctggtg cataccataa taaacttgtg ccgttgaccg
      601 tgtaccggat gatgcttcct tcgaaatcaa catcgatgtt ggcagtgaag gtggcttgaa
      661 tttttgtcgc atcaaacgta cctgctggga cagttatggt ttcggtaccg agctcttgca
      721 tagtgagcgc gaatgtgccc ggggattgag tttgttcacc aggcatggcg actgcgcctt
      781 gcatggaaag gtcgtatttc cattgcatac cgggggtgag tgtgcgtggc aggctgactc
      841 cagagacctg gattgtcttg aaatcggtga tcatgttttg catggagacg ctggctgttg
      901 tgcccccgcc aagttgttga gcaatatagc cttctggggc acaaatccaa ttttgcgtta
      961 gagacagagc cgggaattgt gaggtgagta caaagccatc tggcgtaaga ctggtgatgg
     1021 tgtcggtgta agcgaaatca ccattgggtc cgccgctgct gaaataggac caggatgcat
     1081 tttgtttgac aggaaaaagt ggattatcac aaagctgact tactatatcg gaagtcgtct
     1141 gcccggcggg tggtaggacg gttggtaacg aaatatttga agattgctca ttgcccccgc
     1201 atgcagtgag tagaaccaga actaggatag tgatgacaag ttgatgagtc tggcgcatgg
     1261 aatttatttc catttcggaa gagagattgt gaacgtgctt ccttttcccg gctggctgtc
     1321 cacgtctaac atgccgcggt gttgttgaat gacctgacga gtgatcgaaa gtccaatccc
     1381 caaaccatcg taaatttcgt cgttactttt ttcaagatga aagaaacggt caaaaatacg
     1441 tggacggatc tgcgggtcaa tgccgatgcc ataatccacc acgcttacac tgacataatt
     1501 gcctaagtct gcaaaacgaa tttctactgt gccgccatat gggctgaact taatggcatt
     1561 atcgactaac cctgcaattg cgcgctctag agaaggctca tccccttgaa ccgatgggat
     1621 attggcgctt ccacgtactt tgatatttat gctgcgagtc tttgctttgg cttgatactt
     1681 ttcaacaaca ccctctacaa catgatacaa gttaagtgct tggaaatcac gtagcacaag
     1741 gtcttgttct tgcaagaaaa gcaaatcatt ggttaagttc acgatctgat ccacgttgcg
     1801 gctgacgatt tcaatagtcg actgcaactg ctcgccagtg agaacccctt tttttagagt
     1861 atgtaaatat ccactggcaa ccatcaacgg agttcgcagt tcgtgggcga tgctggttag
     1921 gaactcatta cgcgccatat cttgctcagc aagtttttga tatgcatccg cgatctcacg
     1981 agcgcgtaaa cgtaaatctt cgatagcgag ctggtcgttc gctcttaacc gattgctgat
     2041 ctctgccacc attgcggtgg ctacgctggg actgtgtttt aagactcggt cgaagccttg
     2101 cttatcaagt tccagcacca cagccggcgt tagcgattga accgtggcgg cacgtggcgc
     2161 gttatggatt aacgccatct cgccgaagaa gtctcctgca atcagagtct taagcagtct
     2221 tttttcttta ttattaatgg tctttgtaac ttcaaaatcc ccttcgagga tcatataaaa
     2281 agtatgttca atcgcatctt cctgacatag aatcgtacca gccgggtagc tcttgatttg
     2341 gctgttgacg atgatctctt gcacttcctc cggtttgatg ccgggaaaag cacgcggaat
     2401 gattctggca ggggtgcgaa gtgtggtcat gcttatagat actgatccta cattgatttt
     2461 agcacgggca ggttatctgt tattcaccca ttaggactat tcactttgct tttttcttaa
     2521 ctgtttctca agttctgctg gagtaatgtg taattcctct tcacgtgcgt gaatcgactt
     2581 aattgcactt tcataccagc ctgcaatcca ctcctgccgg atgggtttct ctgccttgag
     2641 atagcgaata aacccaatca catcgatgag gcgcatatca tccagcgtgg aaacctcttc
     2701 cataattatt ttttccagat cattcatggt tcctcctaaa tttgaacaca aatagtaaat
     2761 ggagaaatat ataggatttc ctcaatttgg atttgctagg aaaccggttt atagttaagg
     2821 ggattaccca aattgtactg tgttttatta tagagtggta agaatttagg atgggatttc
     2881 atccaaattc ttgtccaaaa tccctacaac cttttaggag tcttccttgg aaaaaccaat
     2941 tgaacgtcct tccattcttc aaacttacac tcgtag
//
LOCUS       DAOQJE010000437         2975 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_22435_length_2975_cov_7.450685, whole genome shotgun sequence.
ACCESSION   DAOQJE010000437 DAOQJE010000000
VERSION     DAOQJE010000437.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2975)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2975)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2975
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22435_length_2975_cov_7.450685"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..242)
                     /locus_tag="PLT08_11425"
     CDS             complement(<1..242)
                     /locus_tag="PLT08_11425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE05122.1"
                     /translation="MNPLQPDTPSSERPEDDSLNPLISIRSILLSEVFERLRDIERQI
                     EASKNQSQSDDESLRQQLDELLAELDRLQRVAREADA"
     gene            complement(342..1037)
                     /locus_tag="PLT08_11430"
     CDS             complement(342..1037)
                     /locus_tag="PLT08_11430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE05123.1"
                     /translation="MGKSNSSVETETQPAAATPPLAGIEVKADTPEPTADELGRLRDI
                     LFGSQSRTLEKRLSDLESGLRSLRQEMTNLLQDQVGALSESTSTQFTETRRDLNEKID
                     KQGMDQSAQLRASQKELTEKLEKQSAEQSAQLSAGQKQLSDNLEKLAADLLRQLRETH
                     KELSDRLDKQNAEHMERTRTLQTEARQRDDGLRQELLSIAASLENKKTSRQDLGQMLM
                     ELGLRLRQETDNS"
     gene            1393..2235
                     /locus_tag="PLT08_11435"
     CDS             1393..2235
                     /locus_tag="PLT08_11435"
                     /inference="COORDINATES: protein motif:HMM:NF018506.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015144 - carbohydrate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0034219 - carbohydrate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GRP family sugar transporter"
                     /protein_id="HNE05124.1"
                     /translation="MQSIILGLLSALSWGAGDFTGGLAARKVGAYRSVFYAEVIGVIF
                     LFTVLLFTNEPLPDTRAQIFAILAGVFGTLGLMLLYHSMTLGLMSIAAPVSALLAAVL
                     PVLVGVFTEGLPDALTVIGFGFALLAVWLVSQSEGGVKDILSHLSDLKLPLLAGIGFG
                     FYFVLMHQATRNGGAIWHMVFSRSGGMMLIIAYLLVTRSSWKIDLSAIPVISLNGILD
                     LGGNFFFILAGQAGRLDVASVLSSLFPGATVLLAWIFLKERLNRNQWIGVVCALIAIV
                     LMTI"
     gene            2315..2947
                     /locus_tag="PLT08_11440"
     CDS             2315..2947
                     /locus_tag="PLT08_11440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE05125.1"
                     /translation="MNLISEHNQALQQTRYFTAEDLDANRKGQYSQDQVDQAKESRDY
                     IQKNQGKYQNKGWLISLIFGSFLCFFAVVLYFLGVIDMLQNTLGNLFLPVMAGACLLG
                     ALFVFVIVPRSYQSSVDMYKSMGTSLEEQPLGQIQVIEARADTYESRMGVNQRGHRSS
                     HRVSYVLKMDSITFNVTDTLMNTIEKKRLYRVYGVNNGGYWQLLSMETLE"
BASE COUNT          722 a          669 c          685 g          899 t
ORIGIN      
        1 gcatccgctt cacgagcgac acgttgcaag cggtcaagct ctgccaacag ttcgtctaac
       61 tgttggcgga ggctttcatc atcagattgg gattgatttt tggaggcttc gatctgccgt
      121 tcaatatcac gtagacgctc aaatacttct gagagtagga ttgaacgtat ggaaattaac
      181 ggattaagag aatcgtcttc gggtctttca gatgatgggg tgtctggttg aagaggattc
      241 atgaaatttc ttaaatcgaa aaagctgtag gtgtcctagt aagccgtcac ctgataggtt
      301 gaaaataata aacaaggtat tgcacatgca atggaagggg atcaggaatt atcagtttcc
      361 tgtcgtaaac gtagccccaa ttccatgagc atttgaccta ggtcttggcg agaggttttt
      421 ttattttcta aagaggctgc tatggaaaga agctcttgcc ttaagccatc atcacgctga
      481 cgggcttctg tttgcagagt gcgtgtccgc tccatgtgct cagcgttttg cttatctaaa
      541 cgatcgctga gttccttatg agtttcgcgg agctggcgca ggagatcagc agcaagtttt
      601 tccagattat cgcttaattg tttttgacca gcgcttaatt gagcagattg ttctgctgat
      661 tgtttttcca acttctcagt gagctctttt tgcgaggcgc gcagttgggc agattggtcc
      721 ataccttgct tatcgatttt ttcattaaga tcgcggcgag tttctgtaaa ttgtgtgcta
      781 gtggattcgg agagcgcccc gacttggtct tgcaataggt tggtcatttc ctggcgtaga
      841 gaccgaagcc cagactccag atcgctcaat cttttttcaa gggtgcgcga ttgtgagcca
      901 aacaaaatat cgcgcaaacg ccccagttca tcggctgttg gttcgggtgt atccgctttt
      961 acttcaatac cagcaagtgg aggcgtcgcc gcggcgggtt gcgtttcggt ttctacggaa
     1021 gaatttgatt tgcccatttt atttgcttcc tttcgaaaat ttatcaaatt atacacttct
     1081 tcaaaatgcc cggcgactgc aaattagaaa tggatttgct agcgcccgcc caagccttgt
     1141 tgatataaaa aactcacctg caaaacctaa gtttcgcggt gagaaatatg ttttcgaaag
     1201 atcaattaat taaaacgatg aggtcgtccc tctgaaggac gacctcatca actctctcct
     1261 accaataaaa ataccagtaa aacaccaata aagtaccaat aaagatattt gtattctaca
     1321 tgtagctggg gtcttatgca attagacaaa agtactaggg cggtataatc ccgcccacat
     1381 taggagttta ctttgcaatc aattattttg ggacttcttt ctgcactcag ctggggcgcg
     1441 ggtgatttta ctggtgggct tgctgcccgt aaggtcggag cctatcgctc tgtattttat
     1501 gccgaagtga tcggcgttat tttcttattc acagttttac tatttaccaa cgaaccttta
     1561 ccagacacac gcgcgcaaat ttttgcgatt ttggcaggtg tctttggtac cttagggctc
     1621 atgctccttt atcattccat gacccttggc ttgatgagca tcgcagcccc ggtctccgct
     1681 ttgctggcag ctgttctccc cgtattggtt ggggttttca ctgaaggctt gccagatgct
     1741 ctaaccgtga tcggatttgg cttcgccttg ctcgcggtct ggttggtctc ccaaagtgaa
     1801 ggcggcgtga aagatatcct ctcgcatctt tcggacttga aactgcctct cctcgcaggc
     1861 attggattcg gcttttactt tgtcctcatg caccaagcca cccgcaatgg tggtgcaatc
     1921 tggcatatgg tcttctctcg ttctggtggc atgatgctta tcattgctta cttacttgtc
     1981 acccgctctt catggaaaat agacttatct gccataccag tcatctcctt gaacggtatt
     2041 ctcgaccttg gcggtaattt cttctttatc cttgccggtc aagccggtcg tttggatgtg
     2101 gcatctgttt taagctcact atttcccggt gcaacagtcc tcctcgcttg gatattcctc
     2161 aaagaacgcc tcaatcgtaa tcaatggatc ggagtcgttt gcgcattgat tgcaattgtc
     2221 ttgatgacta tctaatgccc acgatggggg atagcctctc gaaatgaaat caatagaata
     2281 gtccctactt attcttaaat ggtagagagg ttttatgaat ttaatatccg aacataatca
     2341 agcattacaa caaacccgct actttactgc cgaagacctt gacgcgaacc gtaagggaca
     2401 atactcccaa gatcaagtag accaggcgaa agaatcgcgt gactacatcc agaagaacca
     2461 aggcaaatac caaaataaag gctggttaat ttcattgatc ttcggtagct ttttgtgttt
     2521 ctttgccgtt gtgctctatt ttcttggtgt tatcgatatg ctccaaaata cgctaggtaa
     2581 cctctttttg ccagtgatgg caggagcatg ccttttgggc gctttgttcg tttttgtcat
     2641 tgtgccgcgc tcttatcaat cctcagtgga tatgtacaaa tccatgggta cgtcgcttga
     2701 agagcaacca cttggacaaa ttcaagtgat cgaagcgcgg gcagatacct atgaatccag
     2761 aatgggcgtt aatcagcgcg gacatcgttc atcgcatcgg gtttcatacg tcctgaaaat
     2821 ggacagcatc acgtttaatg tcaccgatac attaatgaac acgattgaga aaaaacgctt
     2881 gtatcgggtg tatggcgtca ataatggcgg atattggcaa cttctcagca tggagacatt
     2941 ggaataatct atcgtccacg gaatctgtgg tacac
//
LOCUS       DAOQJE010000438         2974 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_22450_length_2974_cov_5.224734, whole genome shotgun sequence.
ACCESSION   DAOQJE010000438 DAOQJE010000000
VERSION     DAOQJE010000438.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2974)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2974)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2974
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22450_length_2974_cov_5.224734"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..347
                     /locus_tag="PLT08_11445"
     CDS             <1..347
                     /locus_tag="PLT08_11445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE05126.1"
                     /translation="AKALVYSSYPFPDRETTCGIFVLNAPGNGTIIVWGFVILSLLGM
                     GIGLYGVHQFQGPVKRGADVFKQFLFLTIVVLTGFLSIYISSWLLGILVLVVSILFVI
                     ISTGIVLGRHHI"
     gene            401..2377
                     /locus_tag="PLT08_11450"
     CDS             401..2377
                     /locus_tag="PLT08_11450"
                     /inference="COORDINATES: protein motif:HMM:NF013324.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HNE05127.1"
                     /translation="MNILVTLCLGGLGVLFLVLLTGIRFIPNNRIGIVEKRFGGKSLK
                     SGLIAINGETGFQPQVLRGGLHYRMPIQYAIHIAPLVTITQGKIGYVFARDGAPLEPA
                     QVLASNVTAHDFQDVTTFLKNGGQKGPQRLILREGTYAFNLMQFVVITEERVYSLPLS
                     RDDTAVIVRMAEVIKERDGFRPVIIKDGDDKIGIVTVHDGPSLPQGEIIAPVVGDNPE
                     KADEYHNKFQDADRFLKANGMRGRQLQVLVEGTYYINRLFATVEMIHKTIIDVGNVGV
                     VVSYTGEQGVDLSGSEYRHGELVTRGQRGVWSEALLPGKYPFNTYAGKVIPVPTTNFI
                     LKWIKNEVGSHNFDENLTEVSLITKDAFEPSLPLSVVVHIDYKKAPLVIQRFGDIKRL
                     VEQTLDPMVAAYFKNVAQTRTLIQLLQERSAIQQQSSLEMKEKFEHYNLELEEVLIGT
                     PSSPAGDKQIETILTQLRQRQIAEEQVETYSQQEKAAVKERELREAESRAAMQTKMTE
                     AELSISIQSNQGKAEYERSIQQAQQIRALAEAEAEKVARIGIAQALATEEQVRAYGGP
                     QFQVTQQVLNRFAEAIQQSGVDVVPRVVVGGGNGENGGNQSANIMEGLLTMLLSERFG
                     VSGEAGTTQRSPDVEAMREKILKDMQKKDDTKKA"
     gene            complement(2401..2922)
                     /locus_tag="PLT08_11455"
     CDS             complement(2401..2922)
                     /locus_tag="PLT08_11455"
                     /inference="COORDINATES: protein motif:HMM:NF024698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNE05128.1"
                     /translation="MAIILETERLILRHQVLSDLDDLWALYQDPEITKYIPDAPRSRE
                     EAQKELEWHMHGHPRRPELGLWATIHKETGKFIGRCGLLPWEIDGQNEVEVAYTIAEA
                     YQGQGLGSEAALAILNYGFSKLKLSRLICLIDPENIPSQKVAEKMGMRFEKESRDEYG
                     MFWIYSIKKEAGN"
BASE COUNT          750 a          741 c          743 g          740 t
ORIGIN      
        1 ttgcgaaggc acttgtctat tcttcctatc ctttccctga ccgtgaaacg acctgcggta
       61 tttttgtgct aaacgccccc ggtaacggga ctataatcgt ttggggcttc gttatattga
      121 gcctgcttgg gatgggaatc gggctttatg gggtgcatca gttccaaggt ccagtaaaac
      181 gaggcgcgga tgtgttcaaa cagttcctat tcctgaccat tgtcgtatta accgggttcc
      241 tttcaattta tatcagttcg tggctgttgg gcatccttgt gctggttgtc tccatcctgt
      301 ttgtgattat ttccacaggc atcgtactcg gacgccacca tatctaaaat tggaaacctg
      361 aatacgcctc acagcgtatt tagatggtga aggagaaaaa atgaatattc ttgtaacgtt
      421 atgtttaggt gggcttggcg ttcttttttt ggtcttactg accggtatcc gctttattcc
      481 aaacaaccgt atcggtatcg tggaaaaacg cttcggtgga aagtcgttga aatctggctt
      541 gatcgccatt aatggcgaga cgggtttcca gccacaggtg ttgcgtggtg ggcttcatta
      601 ccgcatgccc attcagtatg cgatccacat tgcacccttg gtgaccatta cccaaggcaa
      661 aatcgggtac gttttcgcgc gcgacggtgc tccgcttgaa ccagcacagg tcttggcttc
      721 caatgtaaca gcgcacgatt ttcaggatgt gaccacattc ttgaaaaatg gcgggcaaaa
      781 aggtccgcaa cgcttgatct tgcgtgaagg tacatatgca ttcaacttga tgcagttcgt
      841 ggtcattact gaagagcgcg tgtactctct gccgttgagc cgagatgata cggctgtcat
      901 cgtgcgcatg gcagaagtga tcaaagaacg tgatgggttc cgcccagtca tcatcaagga
      961 cggtgacgat aagatcggta ttgtgaccgt gcatgacggt ccgtctttac cgcaaggtga
     1021 gatcattgcc ccagtggtag gtgataaccc agagaaagcc gatgaatacc acaacaagtt
     1081 ccaagatgct gaccgcttct tgaaagcaaa tggtatgcgt ggtcgccaac tccaagtgtt
     1141 ggtagaaggc acatattaca tcaaccgttt gttcgccact gttgagatga tccacaagac
     1201 catcattgac gtagggaatg taggtgtggt ggtttcttac accggtgaac aaggcgttga
     1261 cctttccggt tcagaatacc gacacggtga gctggtcacc cgtggacaac gtggtgtgtg
     1321 gagtgaagcg cttctaccgg gtaagtatcc gttcaatact tatgcaggca aggtcatccc
     1381 tgtgccaacc actaacttca ttttgaagtg gattaagaac gaagtcggtt cgcacaactt
     1441 cgatgagaac ctgaccgaag tctcgctcat caccaaggac gcgttcgagc cttccctgcc
     1501 gctttcagtc gtggttcaca tcgactataa gaaagctccg ctggtcattc aacgcttcgg
     1561 cgatatcaaa cgcctcgttg aacaaaccct cgaccccatg gtggcagcat acttcaagaa
     1621 cgttgcccag acgcgtaccc tcattcaact cttgcaggaa cgtagtgcca ttcagcaaca
     1681 atccagcctg gagatgaagg aaaaatttga gcactacaac cttgagttgg aagaagtgct
     1741 aatcggtaca ccttcctcac cggcaggtga caaacagatt gaaaccatcc tcactcagtt
     1801 acgtcagcgc cagatcgcgg aagagcaggt agaaacctac tcgcaacaag aaaaagccgc
     1861 ggtgaaggaa cgcgaactac gtgaagccga atcccgtgct gcgatgcaga ccaagatgac
     1921 ggaagccgag ttgagcatta gcatccagtc gaaccagggt aaagccgagt acgaacgctc
     1981 catccagcag gcgcaacaga tccgtgcctt ggcagaagcc gaagccgaaa aggtcgcccg
     2041 tatcggtatt gcgcaagcgc ttgccaccga agaacaggtg cgcgcgtatg gtggtccgca
     2101 gttccaggtg acgcaacaag tgctcaatcg ctttgctgaa gccattcaac aatctggtgt
     2161 ggatgtggtt ccgcgtgtgg tggtcggtgg cggaaatggc gagaatggcg gaaaccaatc
     2221 tgccaacatc atggaaggct tgctgaccat gttgctctcc gaacgctttg gcgtctctgg
     2281 tgaagcgggc acgacccaac gctcacctga tgtggaagcc atgcgcgaga agattttgaa
     2341 agacatgcaa aagaaagacg acacgaaaaa agcctaagtc ataaaaaaag aagctggcaa
     2401 ttagttgcca gcttcttttt ttattgaata tatccaaaac attccgtatt catcccggct
     2461 ttctttttcg aaccgcatcc ccattttttc agcaactttt tgcgaaggga tattctccgg
     2521 gtcgatcaag caaataaggc gggataattt caatttactg aacccgtagt tcaggatggc
     2581 taacgccgcc tcactcccca agccttgacc ttgatacgcc tcagcgatcg tatatgccac
     2641 ttcgacctcg ttctgcccgt cgatctccca aggtaaaagt ccgcagcgac cgataaactt
     2701 gcccgtttct ttgtgaatgg tcgcccacaa acccaactcg gggcggcgcg gatgtccgtg
     2761 catatgccat tcgagttctt tctgtgcttc ttcgcgcgaa cgcggcgcat cgggaatgta
     2821 ttttgtgatt tcagggtctt gatacaacgc ccaaagatca tccaagtctg aaagaacttg
     2881 atgccgaaga ataagacgct cagtttcaag aataatagcc atcttatgcc ttcacaatcg
     2941 catagatttc caaatcgctg ggtttgcctt tcca
//
LOCUS       DAOQJE010000439         2968 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_22552_length_2968_cov_3.309990, whole genome shotgun sequence.
ACCESSION   DAOQJE010000439 DAOQJE010000000
VERSION     DAOQJE010000439.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2968)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2968)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2968
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22552_length_2968_cov_3.309990"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(635..1486)
                     /locus_tag="PLT08_11460"
     CDS             complement(635..1486)
                     /locus_tag="PLT08_11460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE05129.1"
                     /translation="MDKNPVLLPEFVKFNPLVGLKSFRTSSAGGAWRLMVLAKNLAGN
                     LDHIERETLRQATRLLGVQDKTFRRWLDAARGLNFVSDIQRTSGEWMLILSSNNSIAF
                     SLGCVNHCRVVQVPIENLFAKNWRAIVFAAWQAGFTGNGERLVSQKKQYEITGIQEQT
                     QRQFNKAAGVQSQKNYAISNIHANGYGAVLEFGNRAGLFQYWNKETHQKHLGWRIPNS
                     RYFPLFGTGGSYKTRRTMSLFNRTAVQYAASMKAIRKNGVKFSEMYVFDRTSQNGNNL
                     WIHLPQK"
     gene            complement(1493..1957)
                     /locus_tag="PLT08_11465"
     CDS             complement(1493..1957)
                     /locus_tag="PLT08_11465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE05130.1"
                     /translation="MSDCSTDSKPDLFQIHVAGRHVGDVISGVFKKRISGSRHILRKP
                     RALALSIESLKQAEQVGAHEIEITDKESKKVYSCSVEHFKEHAFPLQRGGFEPQLALT
                     LDLFDVSSPLDYSSYALRQGEIKQTRSNGRRTRNPRGVRLESPRQMLFKGMM"
     gene            complement(2035..2223)
                     /locus_tag="PLT08_11470"
     CDS             complement(2035..2223)
                     /locus_tag="PLT08_11470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE05131.1"
                     /translation="MQATDKFSMRVTPEDRENFAELKRLFKRPSQSDAIRHVVRETLK
                     AFGKLENPKQGQKTRSRK"
     gene            2615..>2968
                     /locus_tag="PLT08_11475"
     CDS             2615..>2968
                     /locus_tag="PLT08_11475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE05132.1"
                     /translation="MAKTSKTSKTTKAKTSPQKAIEPYEHRDKDRLNNPPVGLVNADS
                     EPEEPRKTYAYDPHLDPSLQWAGKAERTSFDVPTVNLHVHERIDARTIIEAARKLEAK
                     PEATQLSLFAAPTENP"
BASE COUNT          763 a          769 c          591 g          845 t
ORIGIN      
        1 aatcaatcgg gtatttgcaa tccacctttt ctttgcttgc tctggttatg gtgtagataa
       61 gcaatacatt ggaagggaaa tcccccgaac ctttgacctc ataaccaatg cttgatattt
      121 tttcaccgtt tcgactccaa gttacttgac cgcgccagcc tacgctcaaa tccttcttga
      181 taattgaaag gggcaatcga cggcaatctt cagccgccag acgcttattg taaaaacccc
      241 atcgtgtgct cccacaccca cccataaaaa ccacctcctg cgatttagcg aaataatcgc
      301 ctgatattag cagtaacctt ccccattaca cccccgcaaa cgctcgcata tatggaaatt
      361 tttttatatt acatcattgg tttgcttgtt cgttttcctg ttttgttcct gtcaccccta
      421 cccaccaacc ctgtttgctt cccacattcc cagcccgttg agcgggcgaa aaaatttttc
      481 agaaaaaatg gaattttgcc ctgcaaaaag cactcgaagg gcaaccgttc aggcagtcgc
      541 ttggcatctt gctctattcc caccagtacc aagcacagag cagggaataa atacagtgcg
      601 gattaagtac cgataggttt tgtccacaaa aagtttattt ttgaggcaaa tgaatccata
      661 agttattacc gttttgtgaa gttcggtcga agacatacat ctcggaaaac ttaacaccgt
      721 tcttacggat tgccttcata cttgccgcat attgcaccgc tgttcgattg aatagactca
      781 tagttcttcg cgtcttataa gaaccgcctg ttccgaacaa agggaaataa cggctattgg
      841 gtatccgcca gcctaaatgt ttttgatgtg tttccttgtt ccaatactgg aataaacctg
      901 ctcgatttcc aaactccaac accgcaccat atccatttgc atggatattt gaaatagcat
      961 aatttttctg gctttgaacg ccagccgcct tgttaaattg tctttgtgtt tgctcttgaa
     1021 ttcccgtaat ttcatattgc tttttttgag atacgagtct ctcaccgttc cctgtaaacc
     1081 ctgcctgcca tgcagcaaat acaatggcac gccaattctt tgcaaataaa ttctcgattg
     1141 gcacttgcac aacacggcaa tgatttacac accccaaaga aaaggcgatt gaattattac
     1201 ttgagagtat cagcatccat tcgccgcttg tacgttgaat atctgacaca aagttcaacc
     1261 cgcgagccgc atccaaccac ctgcggaaag tcttatcctg aactcccaac agtctggtag
     1321 cctggcgaag ggtttcccgt tctatgtggt cgagattgcc ggcaaggttc ttggcgagta
     1381 ccattaaacg ccatgccccg cctgcacttg aggtgcgaaa agacttcaac ccaaccaacg
     1441 gattgaattt cacaaattca gggagcagga cggggttttt atccatacag tattacatca
     1501 tccccttaaa taacatctgg cgtggactct ccagccgcac accgcgcgga ttccgcgttc
     1561 gcctgccatt acttcttgtc tgtttgattt cgccttgcct cagggcataa cttgaataat
     1621 ccagcgggga ggatacgtcg aagaggtcaa gcgtcaaagc caattgaggc tcaaagcctc
     1681 cgcgttgaag cggaaaggcg tgctctttga agtgctcgac cgagcaggaa tatacttttt
     1741 ttgattcctt gtcggttatc tcgatttcat gcgcgccgac ctgctctgcc tgtttcaaac
     1801 tttcaatgga caaggcaagc gcgcgcggct tgcgtaagat gtgacggctt cctgaaatcc
     1861 ttttcttgaa taccccgctg ataacatccc ctacatgacg acctgctaca tgaatttgaa
     1921 acaaatcagg ttttgagtct gtgctacaat ctgacataga aaatatcctt tcattcgcta
     1981 agtgtttggc tgttttcgac ctgacccgcc gctcgaacgg cgggtctttc gtttctattt
     2041 gcggcttcgg gtcttttgac cttgttttgg attttccaat ttaccaaatg ctttcagggt
     2101 ctcccgtacc acatgacgaa tggcatccga ctgtgatggg cgtttgaata accgcttcaa
     2161 ttcggcgaag ttttcgcggt cttctggagt aacacgcatt gagaatttat ccgttgcttg
     2221 catatttttt ccttttttcc tttctatttt atgatttgat taaataaaaa caggcggaca
     2281 tccgattaca aggggtttta agtttcccca gcaattactt gctggttaca cctccttgtt
     2341 tgtcgaacat ccgcctgtca tgctgcatta ttttacaaga acctccacaa cacatttaat
     2401 ccaaatctct ggataaatgt atttcgtgaa tgttgtaagg ttcattcgca acaatgaaaa
     2461 gcgaccactg ctcatttgtt cggattttac caaaagtatg gacaaggtca agtcttttcc
     2521 tgtcatgttt ttgtccgtac attgtctgca ttattgttta taattgcagt ctcttcgtct
     2581 tataaatttt gaacacctat gagggactcg gtacatggca aagacaagca aaacctccaa
     2641 gacaaccaaa gcaaagacct ctccccaaaa agcaatcgaa ccttacgaac accgtgacaa
     2701 agaccggttg aataacccgc ctgtgggact tgtaaacgcg gactctgaac ccgaagagcc
     2761 gcgcaagacc tatgcctacg acccgcacct tgacccaagc ctgcaatggg ctggcaaagc
     2821 cgagcggaca tccttcgacg tgccgactgt taacttgcat gttcacgaac gcattgacgc
     2881 gcgcactatt atcgaagccg cgcgcaaact ggaagccaaa cccgaagcga ctcaactctc
     2941 tttattcgcc gcgccgaccg agaatccc
//
LOCUS       DAOQJE010000440         2965 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_22588_length_2965_cov_4.164261, whole genome shotgun sequence.
ACCESSION   DAOQJE010000440 DAOQJE010000000
VERSION     DAOQJE010000440.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2965)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2965)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2965
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22588_length_2965_cov_4.164261"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1077
                     /locus_tag="PLT08_11480"
     CDS             <1..1077
                     /locus_tag="PLT08_11480"
                     /inference="COORDINATES: protein motif:HMM:NF012447.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid lyase"
                     /protein_id="HNE05133.1"
                     /translation="HMALPIIGEGKAEYQGEVMSGAEAFKRAGLKSITLAAKEGLALT
                     NGTTIMTAMGVLQTLRAERYSQTADIAGCLSLEALNGTDLAFDERIHNLRPFPRQIEC
                     ARYLRGVLKGSEFMREHSSLNVQDAYTLRCIPQVHGAVRDAIAYARWVFEIELNAVTD
                     NPLIFIDDKDEITVLSGGNFHGEPLAIAMDYLAFSVAELGNISERRIMRLTDECSNRN
                     VLPAFLTNEGGLNSGFMILQYTAAALATENKVLAHPASVDTIPTSANVEDHVSMGATA
                     AVKLRGVLDNVEKTLAIELMSAAQGVDFRKQVIGAEKKLGEGTRDVYALIRKHIPFIE
                     ADTVMYEYMETMRQLVADGKVLSI"
     gene            1231..>2965
                     /locus_tag="PLT08_11485"
     CDS             1231..>2965
                     /locus_tag="PLT08_11485"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HNE05134.1"
                     /translation="MDVFFVISGYLITTIILGDIAAGKFTFSNFYERRARRIIPALIV
                     VMLVCVPFAYYWMLPSQLTEFSQSLIAVSLFGSNILFWLQTGYFATASDLKPLLHTWS
                     LAVEEQYYLFFPLLIIFISRFGRRVMLAILVLLGLVSILFAQFQVRRDPDFAFYLILT
                     RLWEILIGSFVSIYLFQKPDVKFDSLIEQALSMLGLVSILYAVIAFNDRTPFPSFYTL
                     VPTLGTALIILFASRETFAGKFLSNKLLVGVGLISYSAYLWHQPIFVFARLISIEEPN
                     EILMLVLSFLTLALAYLTWKYIETPFRNRSKFDRNKIFLFSGASSFLIVVIGGIGVIG
                     SGFGYRIAPNGMYYSDVGQVLQENYGLNKECDYKAYRPFERYTIIDQCRTGKNPKILI
                     WGDSFAMQLVPGILASQPNAKIIQMTKSGCGSIIAMQVVRTPRYADDCINFNNDVVGW
                     LKSTKSIRYVVMSSRLSYYLDSENYIAMNNKVVPVDKKIVLTQMEETLALITSLGKTP
                     IFFGPLPTNGKNIGNCLIKKAFYLDGIDCRIETVQFKDSLIPDFLKKVEIQYPVIWLS
                     DAICDDRYCYPE"
BASE COUNT          773 a          554 c          719 g          919 t
ORIGIN      
        1 catatggcgt tgccgattat tggcgaaggc aaagcggaat atcaaggcga ggtgatgtct
       61 ggggcagagg cattcaaacg cgcagggttg aagtctatta ctctggcggc gaaggaaggt
      121 ttggcgctga cgaatggcac gaccattatg acggcgatgg gtgtcttgca gaccttgcga
      181 gcggagcgct atagtcagac cgccgacatt gccggatgcc taagcctcga agccttgaat
      241 ggaacagacc tggctttcga tgaacgcatt cataacctgc gaccgtttcc gcgtcagatc
      301 gagtgcgccc gttatttgcg tggtgtattg aaaggcagcg aattcatgcg tgaacattct
      361 tctttgaatg ttcaggatgc gtacacacta cgctgcatcc cacaggtgca tggagccgta
      421 cgtgatgcga ttgcatatgc acgttgggtc tttgaaattg aattaaatgc agtgacggat
      481 aatccgctga tctttatcga tgataaagac gagatcacag ttttatctgg cggtaatttt
      541 catggcgagc cgctggcgat tgcgatggac tatctcgcgt tttctgtggc ggagctggga
      601 aatatctctg aacgtcgcat tatgcgcctg acggatgaat gctcgaatcg aaatgtgttg
      661 cctgcatttc tcacgaacga aggcgggttg aactccggct ttatgatcct gcaatatacc
      721 gcggctgcgc ttgcgaccga aaacaaagtg ctggctcatc ctgccagcgt ggacacgatt
      781 cccacatccg ctaacgtgga ggaccatgtt agtatgggtg caacagctgc ggtcaagttg
      841 cgaggcgtgc tcgataacgt cgagaagact cttgccattg agttaatgtc cgctgcgcaa
      901 ggcgtggact tccgcaagca ggtcatcggc gcggagaaaa agttgggcga aggcacgaga
      961 gacgtctatg ccctgatccg caaacacatc ccgttcattg aggcggatac ggtcatgtat
     1021 gaatatatgg agacgatgcg ccagctcgtg gcggatggga aggttttgtc aatataattt
     1081 ggtgactaat tggtcacata cacatcattg cctgattgag agaaaagatg aaatacagga
     1141 ctgaaattga cggactgaga gctgtagctg ttttaccggt tatttttttt tcatgctgga
     1201 tttgccttct ttagtggtgg atacattggg gtggacgttt tttttgtcat cagcggttac
     1261 cttatcacaa ctattatcct cggtgatata gcagcaggta aatttacttt ttctaatttt
     1321 tatgaaagaa gggcaagaag aatcatccca gccttaatag ttgttatgct agtatgtgtg
     1381 cccttcgcct attactggat gctgccgagt caattgacag aattttctca aagcttgatc
     1441 gctgtttcat tgtttggttc gaatatactt ttctggctac aaaccggtta ctttgccaca
     1501 gcgtctgatt tgaaacctct tcttcatact tggagtttgg cagttgagga gcaatattat
     1561 cttttttttc ctttattgat cattttcata tctcgctttg gaaggcgcgt tatgcttgcc
     1621 attctcgtgt tacttggttt ggtaagcatc ctttttgcac aatttcaagt cagaagggat
     1681 ccagattttg ctttctattt gattcttact cgtctgtggg agattttgat tggctccttt
     1741 gtatcaattt acctttttca gaagccagat gtgaaatttg acagcttgat agaacaagca
     1801 ttaagtatgt tgggtctcgt gtcaattttg tatgcggtta ttgcttttaa tgacagaaca
     1861 ccttttccaa gtttttatac gcttgtgcca actcttggaa ctgctctaat cattttgttt
     1921 gcttctaggg aaacatttgc cggaaaattt cttagcaata aattgttagt tggagttgga
     1981 ttgatcagct acagcgccta tttatggcac cagccaattt ttgttttcgc aaggttgata
     2041 agtattgagg aaccaaacga gatattaatg ctagttctta gctttttgac attggctctt
     2101 gcctatttaa catggaagta tattgaaact ccatttagaa atagatccaa gttcgatcgc
     2161 aataaaattt ttttgttttc aggggcgtca tctttcctta ttgttgtaat aggcgggatt
     2221 ggcgttatag gttcaggctt tgggtatagg attgcaccca atggaatgta ttactctgat
     2281 gttgggcaag tgttgcaaga aaattatgga ttaaacaaag aatgtgatta caaagcatat
     2341 aggccgtttg aaagatacac gataatcgat caatgtagaa ccggtaaaaa cccgaagata
     2401 ttaatttggg gcgattcttt tgcgatgcaa ttagtccctg gtattttggc gtcacagcct
     2461 aacgcaaaga ttattcaaat gaccaagagt ggttgtggtt ccattattgc gatgcaggta
     2521 gtgcggacac cccgatatgc tgacgattgt ataaatttta ataatgacgt tgttggctgg
     2581 ctgaaaagca caaaaagtat tcgttatgtt gtcatgagtt ctcgattatc ctactatctt
     2641 gatagtgaga actacatcgc gatgaataat aaggttgtac ctgttgataa gaaaattgtt
     2701 ttgactcaga tggaggagac ccttgccttg atcacaagtt tgggaaaaac gccaattttc
     2761 tttggaccac ttccaacaaa tgggaaaaat attggaaatt gtttgataaa aaaagcattc
     2821 tatttagatg gaattgattg tagaattgaa acagtgcaat ttaaagactc cctaatccca
     2881 gatttcttga agaaggttga aatacaatat ccagtgatct ggcttagtga tgcgatatgt
     2941 gatgataggt attgttatcc tgaaa
//
LOCUS       DAOQJE010000441         2961 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_22640_length_2961_cov_5.311081, whole genome shotgun sequence.
ACCESSION   DAOQJE010000441 DAOQJE010000000
VERSION     DAOQJE010000441.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2961)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2961)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2961
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22640_length_2961_cov_5.311081"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            166..1083
                     /gene="fmt"
                     /locus_tag="PLT08_11490"
     CDS             166..1083
                     /gene="fmt"
                     /locus_tag="PLT08_11490"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR00460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HNE05135.1"
                     /translation="MSIRVVFMGSPDFALPSLRALVDRYNVVGVVTQPDRASGRGREL
                     KAPPVKLLTQDLNIPVMQPQKLREPEAMQQLQAWNPDLIVVAAFGQILRKDVLDLPKY
                     GCVNVHASLLPRWRGAAPINASVLAGDEETGVTIMKMDVGLDTGPMLSMEKIRIKPDD
                     TAGALFEALSTLGAKLLIETLPGYINGTVLPQPQPEEGATYAPMLKKEDGRLDFNQPA
                     VELERRIRAMNPWPGAWFEWNGNILKVSRGAVSEAKGKEVGSRLIVEGRPAVQCADGV
                     LILEEVQPPSKKVMPGKAFLSGTRNWESS"
     gene            1135..1884
                     /locus_tag="PLT08_11495"
     CDS             1135..1884
                     /locus_tag="PLT08_11495"
                     /inference="COORDINATES: protein motif:HMM:NF012455.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin"
                     /protein_id="HNE05136.1"
                     /translation="MFNWKVFLAEFIGTFALVFIGAGAGVVQAGLVGVALAHGLTLMV
                     FAYSFGHISGTHVNPAVTFGLALQGVVKWGEAVVYWVAQCLGAISAAGLLHLVVSQLG
                     GTINAGATVGALTDKFPVYAMLIEAVLTFFLLNTILHTAVAGKGGQFAGLAIGLTLTA
                     AILVGGPMTGASLNPARTLGTAVYTSPSLANVNTYVTYLFGPLIGATAAVLIFNVFKT
                     EAPVAEEEISAPVEAPAKPATKRKTTAKAKK"
     gene            1962..>2961
                     /gene="glpK"
                     /locus_tag="PLT08_11500"
     CDS             1962..>2961
                     /gene="glpK"
                     /locus_tag="PLT08_11500"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HNE05137.1"
                     /translation="MTQYVGAIDQGTTSTRFIIFDHNGNVVAVDQKEHRQVYPKPGWV
                     EHDALEIWQRTQEVMAGALAKGGLSSEDLAAIGVTNQRETTLVWDKNTGEQVYNAIVW
                     QDTRTDTIISKFAKQGGQDRFRKKTGLPLATYFSGPKIKWVLDNVKGAKERAKKGDLL
                     FGNMDTWIIWKLTGKHVTDVTNASRTMLMNLKSLDWDAEILKVMGIPRAMLPEIKSSS
                     EVYGYVGAGAPLRGVPVAGDLGDQHAALFGQTCFKAGEAKNTYGTGCFMLMNTGEKPV
                     QSKAGLLTTLGYKIGDKKPVYALEGSIAISGALVQWLRDNLGMIEKSSDVEELAKTV"
BASE COUNT          738 a          638 c          817 g          768 t
ORIGIN      
        1 catggtttat tcctccgaat aagttgtttg aattttaagt attttacaac caatctacac
       61 aacgtgcaac caaactaatt cacccaatgg tactgatacg ttacgaagag ccccgttttg
      121 ctgaaaattc tccttaatta ttgagaccag cggtaaaatc tgcacatgtc tattcgtgtt
      181 gttttcatgg gttcgcccga ttttgctctt ccgagcctcc gcgctcttgt tgatcgctat
      241 aatgttgttg gggtggttac ccagccagat cgtgcctctg ggcgcggacg tgaactgaag
      301 gcgccgcctg ttaagctttt aacgcaagac ctcaatatcc ccgtcatgca gcctcaaaaa
      361 ttacgtgagc ctgaagcgat gcaacagttg caagcttgga atcctgacct tatcgtggtt
      421 gcagcttttg gtcaaatctt aagaaaagat gtattagacc tgcccaaata cggctgtgtc
      481 aatgtgcatg cttcactgtt gccacgttgg cgtggagcgg caccgatcaa tgcatctgtg
      541 ctggctgggg atgaagagac gggcgttact attatgaaaa tggatgtggg gttggataca
      601 ggtcccatgc tttcgatgga aaaaattcgc atcaagccag acgatacagc gggcgcactc
      661 ttcgaggctt tatctacgct gggagcgaaa cttcttattg agaccctgcc tgggtacata
      721 aatggcactg tcttgccaca acctcaaccc gaagaaggtg caacctatgc cccgatgctc
      781 aaaaaagaag atggacgatt agactttaat caaccagctg tggaattgga gcgaagaatt
      841 cgtgcgatga acccatggcc cggagcatgg tttgagtgga acgggaacat actcaaagta
      901 agtagaggtg ctgtcagcga agcaaaaggc aaagaagtgg ggagcaggct tatcgtcgaa
      961 ggaagacccg cagtacagtg tgcggatgga gtcttaattt tggaggaagt ccaaccgcct
     1021 agcaagaagg ttatgccggg gaaggcgttt ttatccggca cacggaattg ggaatcgtcg
     1081 tagcaaattg acaggtatgg ggttataact tccaaattaa tctttggagg atgtatgttc
     1141 aattggaaag tatttcttgc tgaatttatc ggcacgtttg cgttggtgtt tatcggtgca
     1201 ggggcgggcg tggtacaggc ggggcttgta ggcgtggctc ttgcacatgg tttgaccttg
     1261 atggtcttcg cttattcatt tggtcatatt tcgggtaccc acgtgaaccc agcggtgacc
     1321 tttgggttgg cattgcaggg tgttgtgaaa tggggcgaag cggtggtgta ttgggtagct
     1381 caatgccttg gggcgatctc ggcggctgga ttgcttcatt tggttgtctc tcaactcggc
     1441 gggacgatca atgctggagc tacggtaggc gcgttgaccg ataaattccc ggtgtatgcc
     1501 atgttgatcg aagccgtgtt gacattcttc ctgctcaaca caattttgca tacggctgta
     1561 gcaggcaagg gcgggcagtt tgctgggttg gcaatcggct tgacattgac cgctgccatt
     1621 cttgtaggtg gaccgatgac cggtgcatct cttaacccgg cacgcacttt aggaaccgct
     1681 gtttatacaa gcccaagctt ggcgaacgtg aatacctatg tcacatactt gttcggtccg
     1741 ttgattggtg ccacggcggc tgttttgata ttcaatgtgt ttaagactga agcacctgta
     1801 gcagaagaag agatttctgc tccggttgaa gccccagcaa aaccggcgac aaagcgcaag
     1861 acaactgcaa aagcaaagaa ataaaatttg cgcttctttc aagccttcgc cttcaaagcg
     1921 aaggcttttt gttttacaat taatccattg gaggtactct catgactcaa tacgttggcg
     1981 cgattgatca aggcacgacc agtactcgtt ttattatttt cgaccacaac ggcaatgtgg
     2041 ttgcagtgga tcaaaaagaa cacaggcagg tctatcccaa gccgggctgg gtggaacatg
     2101 atgctttgga aatttggcag cgcacgcagg aagtgatggc tggcgctctg gcaaagggtg
     2161 ggctctcttc tgaagatctt gctgcgattg gtgtgaccaa ccagcgcgag acgaccctag
     2221 tatgggataa aaacaccgga gagcaggtct acaatgctat tgtctggcaa gatacacgta
     2281 ccgatacaat catttccaag tttgccaaac aaggcgggca ggatcgcttt agaaaaaaga
     2341 ccggcttgcc attggctacc tatttttcgg gtcccaagat caagtgggtt ttggacaatg
     2401 taaaaggggc aaaggaacgt gctaaaaaag gcgacttgct tttcggcaac atggacacct
     2461 ggatcatttg gaagttgacc ggtaagcatg tgaccgatgt gactaatgct tcgcgcacca
     2521 tgttgatgaa tctaaagtct ttggactggg atgctgaaat tttgaaggtg atgggcattc
     2581 cgcgcgcgat gctgccggag atcaaatctt cgtctgaagt ctatggatat gtaggtgcag
     2641 gagccccgtt gcgtggtgtg ccagtagcag gtgacttggg cgatcaacat gctgcgctat
     2701 ttggtcagac ctgtttcaag gcgggtgaag ccaagaatac ctacggcacc ggttgtttta
     2761 tgttgatgaa cactggcgaa aaacctgttc aaagcaaagc cgggttgttg accacacttg
     2821 gatacaagat cggtgataag aaacctgttt atgccttgga aggctcgatc gcaatttctg
     2881 gggcgctggt gcaatggctg cgagataacc tcggcatgat cgaaaaatct tccgatgtgg
     2941 aagaactggc gaagacggtc g
//
LOCUS       DAOQJE010000442         2951 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_22767_length_2951_cov_7.179213, whole genome shotgun sequence.
ACCESSION   DAOQJE010000442 DAOQJE010000000
VERSION     DAOQJE010000442.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2951)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2951)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2951
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22767_length_2951_cov_7.179213"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..576
                     /locus_tag="PLT08_11505"
     CDS             <1..576
                     /locus_tag="PLT08_11505"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF014069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HD-GYP domain-containing protein"
                     /protein_id="HNE05138.1"
                     /translation="SNRELSFAYEATIEGWSRALDLRDHETEGHTQRVTEMALKLADI
                     MGLSDAEKADLRRGALLHDIGKMGVPDSILHKPGTLTEHEWQVMRQHPSYAYQMLSPI
                     SYLRRALDISYCHHEKWDGTGYPNGLKGNEIPLAARLFAVVDVFDALTSDRPYRKAWS
                     NEETYAFIQSQSGKHFDPQVVQALLKLSEEK"
     gene            581..1060
                     /locus_tag="PLT08_11510"
     CDS             581..1060
                     /locus_tag="PLT08_11510"
                     /inference="COORDINATES: protein motif:HMM:NF013460.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b/b6 domain-containing protein"
                     /protein_id="HNE05139.1"
                     /translation="MNKTPRISILLHWITVLLIFPSAILAKQKELDGLPLNAHTIIGV
                     VLAVTMVIRLTFYIRNFTKKFLIANIVYSLLYIGIFFVLITGVWIAYQRNLIGYLLDP
                     NSAFGRGGFKLLADIHKLGWQILLGWIVLHIGIVSYRQFLKKEAILERMWFTKSNQK"
     gene            1154..1645
                     /locus_tag="PLT08_11515"
     CDS             1154..1645
                     /locus_tag="PLT08_11515"
                     /inference="COORDINATES: protein motif:HMM:NF013460.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b/b6 domain-containing protein"
                     /protein_id="HNE05140.1"
                     /translation="MNTPKRYHPILVTLHWLTVIFMLGAGFLSEEGGNSPINFHMILG
                     ALLLIVMVARLITRFSTARPAALDAGNAFFNKIGELTHLGLYAVAFAILGMGGAIAVK
                     RNLFGYLMDNTAQISRAGFLGDVHHLFYLMAMALIFLHVGAALYHQFMLKDNILSRMW
                     YGK"
     gene            1728..2453
                     /locus_tag="PLT08_11520"
     CDS             1728..2453
                     /locus_tag="PLT08_11520"
                     /inference="COORDINATES: protein motif:HMM:NF014774.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HNE05141.1"
                     /translation="MPSVQLFITCLTDSFFPETGEAVVDILSRLGISVDFTPDQTCCG
                     QPQFNAGLRNDARAIAEHTIKVFEKTSGDIVTPSGSCAHHFRHNLLELFEGDVEWYPR
                     AEAFGKRVFEFTEYLVDKLNVTDLGAVWDGLLTYHPSCHTLRGLNVDRQPRALLQNVK
                     GATLVQLPNEHECCGFGGVMSVEHPELSAEWLKRKISNLETTHAPTLVVTDAGCLMHI
                     MGGLKRQGKKQKVLHIAEILRNT"
     gene            2499..>2951
                     /locus_tag="PLT08_11525"
     CDS             2499..>2951
                     /locus_tag="PLT08_11525"
                     /inference="COORDINATES: protein motif:HMM:NF014628.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LUD domain-containing protein"
                     /protein_id="HNE05142.1"
                     /translation="MNTFRNRIRQSIQNETLQTALDNNTERRLRGKTLAFESIPDWRE
                     RRQRAHRIRADVIDHLDEYLDQFVANAQANGIVIHRAKDAKEAIEIVLKISQSITNHQ
                     LPNTLIAKSKSMVSEEIELNHTLEKHGINVVETDLGEYIVQLRKEKPSH"
BASE COUNT          829 a          715 c          612 g          795 t
ORIGIN      
        1 tctaaccgtg aattatcatt tgcatatgaa gccaccatcg aaggttggtc tcgtgcttta
       61 gacttacgag atcacgaaac agaagggcat acacaacgtg ttactgaaat ggcgttaaag
      121 cttgccgata tcatgggctt gagtgacgct gaaaaagccg atctacgacg cggtgcttta
      181 ttacatgaca ttgggaaaat gggtgtccct gattcaattt tgcataagcc aggcactctc
      241 acagaacacg aatggcaagt catgcgtcaa cacccttcat atgcctacca gatgctttct
      301 cccatctcct acttgaggcg cgcactggat atctcatatt gccatcacga aaaatgggac
      361 ggaacgggtt accctaatgg gttgaaagga aatgaaattc ccttagcggc aagactattc
      421 gccgttgtag atgtatttga tgctcttact tctgatcgtc cctatcgcaa ggcatggtca
      481 aatgaagaaa catatgcgtt tattcagtcg caatctggga aacattttga cccacaagta
      541 gtgcaagctc ttttaaagtt aagcgaagaa aaataaaaga atgaacaaaa ctcctcgtat
      601 ctctatactt ttgcactgga ttacagtgtt actcattttt ccatctgcaa ttctagccaa
      661 gcaaaaagaa ctggatggtt tacctctaaa cgcacataca attattggag tagtgttagc
      721 cgttaccatg gtaatccggc tgaccttcta cataagaaat tttacaaaga aatttcttat
      781 cgcaaatatt gtttatagcc ttttatatat tggtatcttt tttgttctta ttacaggagt
      841 gtggatcgcc tatcaaagaa atttaatcgg ctatttatta gatccaaatt ccgcatttgg
      901 aagaggcggc ttcaaacttc ttgctgatat ccacaaatta ggatggcaaa ttttgctcgg
      961 ttggatcgtt ttgcatattg gcatagtgtc ctatcgtcaa tttctcaaaa aagaagcaat
     1021 tttagaacgt atgtggttta caaaaagcaa ccaaaaataa ttatggggaa attatggaga
     1081 aaacttcaag gattagattt tgtaatccgg ctttaatcat gcagttctat agtatgggca
     1141 aggagagcaa aaaatgaata ccccaaaacg ttatcatccc attctcgtta cgctgcactg
     1201 gcttactgtg atctttatgc ttggtgcagg cttcctttca gaagaaggtg gcaattcccc
     1261 gattaacttt cacatgattc ttggcgcgct tcttttgatc gttatggttg cacgcttaat
     1321 cacccgtttt tccacggcgc gacccgccgc attggatgca ggaaatgcct tcttcaataa
     1381 gatcggtgag ttgactcatc ttggattgta cgcagttgca ttcgccattc tcggcatggg
     1441 tggagccatt gcagtaaaac gcaatctatt tggttatctt atggacaata ccgctcaaat
     1501 cagccgtgct ggttttcttg gtgacgttca tcacctcttt tacctcatgg caatggcatt
     1561 gatcttttta catgtaggcg ctgccctcta ccaccaattt atgctcaagg acaatatcct
     1621 ctcgcgcatg tggtacggaa aatagacatt ccatagtttt cctcttcaac gccccaacaa
     1681 agtatgagct ttgttggggc gttttttatt ggtcgtataa tcgacacatg cccagcgtcc
     1741 aacttttcat cacctgtctc accgattcat tctttcccga aaccggtgaa gccgtcgtag
     1801 acattctctc ccgcctcggc atcagcgtgg actttacacc cgaccaaacc tgttgcgggc
     1861 aaccgcaatt caatgctggc ttacgcaatg atgcgcgtgc tattgcagaa cacaccatca
     1921 aagtatttga aaagaccagc ggagacatcg tcacgccctc cggttcttgt gcccatcatt
     1981 ttcggcacaa tctcttggaa ctctttgaag gagatgtgga gtggtatcca agggcggaag
     2041 catttggcaa gcgtgtcttt gaattcaccg aatatttagt ggacaagctc aacgtcaccg
     2101 acctcggcgc tgtatgggac ggtctactta cctatcaccc atcctgccac accctacgcg
     2161 gactcaacgt ggacagacag ccacgcgctc ttttgcaaaa tgtcaaaggc gcaaccttgg
     2221 tgcaactacc aaatgaacac gaatgctgcg gcttcggcgg cgtgatgtct gttgaacacc
     2281 ccgaactctc ggcggagtgg ttgaaacgca aaatatccaa ccttgaaacc acacacgctc
     2341 ccacacttgt cgtcaccgat gcaggttgtc tcatgcacat catgggcggc ttgaagcgac
     2401 aaggaaagaa acagaaagtt ttgcatatcg cagaaatatt gcgtaatacg tgaaaagtaa
     2461 aacgcgatat cccgtatctg atctccaata cctaatctat gaatactttc cgaaatcgca
     2521 tccgtcaatc catccaaaac gaaactcttc agacagcact cgataacaac accgagcgcc
     2581 gtctgcgagg aaaaacttta gccttcgaat ctattccgga ttggcgcgag agacgccaac
     2641 gcgctcatcg catccgcgcg gatgtgatcg atcatctcga tgagtatttg gatcaattcg
     2701 tcgccaacgc ccaagccaat ggcatcgtca tccaccgcgc caaggatgcg aaagaagcca
     2761 tcgaaatcgt tttgaaaatt tcccaatcaa ttaccaatca ccaattacca aacaccctca
     2821 tcgccaaatc caaaagcatg gtctctgaag agatcgagct caatcacacc ctcgaaaagc
     2881 acggcatcaa cgtggtcgaa accgacttgg gtgaatacat tgttcaattg cgaaaagaga
     2941 aaccaagtca c
//
LOCUS       DAOQJE010000443         2950 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_22786_length_2950_cov_4.712263, whole genome shotgun sequence.
ACCESSION   DAOQJE010000443 DAOQJE010000000
VERSION     DAOQJE010000443.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2950)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2950)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2950
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22786_length_2950_cov_4.712263"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            157..2505
                     /locus_tag="PLT08_11530"
     CDS             157..2505
                     /locus_tag="PLT08_11530"
                     /inference="COORDINATES: protein motif:HMM:NF014442.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006306 - DNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-6 DNA methylase"
                     /protein_id="HNE05143.1"
                     /translation="MQQQHLSERRTEDLTVDLLDIQGWSTNRPPKGSVVRQNEYKAFP
                     TLSNIFVGMSKSGKGDGYPDFLVVDKDTLRPQIIIETKASAKEIDQALSEACAYGNAC
                     RQAGHNVISVGIAGQEKTGIKVAVAGYYKGKWQSILYTDKPISWIPTPTDTQKLLSST
                     NRISLTPIVPRPEILASKADEMNRILREANVKDEYRPAYIGAMMLSLWESHGNISREP
                     SHVLKEVNSYCGTAFSRGGKSELGKSLRVDVANTKLANSAWVILATLEKLNVVAGSVD
                     HDYLGQLYELFFQYTGGNTIGQYFTPRHITRFMADICETSKTDLVIDPACGTGGFLIS
                     TIQRAYDVLGIKYEDAINIVKEKLIGYESEPVTAALCVANMILRGDGKTGIRKDDSFT
                     AADFPIGKCQVALMNPPFPHKQTDVPSQQFVERALEALENRGLLAVILPSSLLVKKDI
                     GEWRRTILLNNTLEAVCQLPDELFQPYASATTSVVLLRKGVPHSKSKKTVFVRLRHDG
                     LTLKKGTRIPRADGLNDIPSATEAILNKRQDAGFSGLAIVSQDDEWSVGAYIPSGAPS
                     IDELKNSVDESLRRLVSFYVRYAQEVATLRSSVKNGAVNVSPYRDMLSSMRLDNAKTI
                     STQKNTIGEIFDIYYGQKELHSRDGIPNGVSLIISPTEQYNGCYGWLDFPNLIKPPFV
                     TVAQTGSIGETFVQLEPCGVNDDCLILLPKEGIDLPLSSYFIASATIRLEKWRFSYGR
                     KLTPARISDFQMTHSPQLEEWINEQIRNWNQIINKSIKAYES"
     gene            2679..>2950
                     /locus_tag="PLT08_11535"
     CDS             2679..>2950
                     /locus_tag="PLT08_11535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE05144.1"
                     /translation="MKKSLNSPLETILESIVILVVWVVFLPIFWAMSEANSENKVLFW
                     LFLFLGLVLIFLQWKFLPIRHIEFDGNFIYVSNYFRKIVIAISEIE"
BASE COUNT          952 a          547 c          589 g          862 t
ORIGIN      
        1 ttcctccccc caaacagaat ccacgcccac ccacacgcag gtaacgcaag ccgttaggct
       61 gcttgctcct ataaattaaa tatgttgtta tagtctggtt tacggggtaa aatagacaaa
      121 atgttctata aacattaagt gataaggaat tttattatgc aacaacagca tttatcagaa
      181 cgccgaacag aagatttaac tgtagactta cttgatattc aaggttggtc tactaatcgc
      241 cctccaaaag gcagtgttgt tcgacaaaat gaatacaaag catttcctac attatcaaat
      301 atttttgttg gcatgagtaa atctggaaaa ggcgacggct acccagattt tcttgttgta
      361 gacaaagaca cattgcgccc tcaaataatt attgagacaa aagcgagcgc aaaagaaata
      421 gaccaagcct tatctgaagc atgtgcttac ggaaatgcct gtcgccaagc aggtcataat
      481 gttatttcag taggcattgc tggacaagaa aaaacaggga ttaaagttgc cgtcgctgga
      541 tactataaag gaaaatggca atctatttta tataccgaca aaccaatttc ttggataccg
      601 actccaactg atactcaaaa acttttaagt tcaaccaata gaatcagttt aacgcctatt
      661 gttcctcgac cagaaatttt agcaagtaaa gccgatgaaa tgaataggat attaagagaa
      721 gcaaatgtca aagatgaata ccgccctgct tatattggcg caatgatgtt atctctttgg
      781 gaatctcatg gaaatataag cagagaacct tcgcatgtat taaaagaagt aaatagttac
      841 tgcgggacag cattttctag gggcggaaaa tctgagttag ggaaaagtct tagagttgat
      901 gtagccaata ctaaattggc taattcagca tgggtaatat tagcaacact agaaaagttg
      961 aatgttgttg caggttcggt tgaccatgat tatttaggtc agttatatga actatttttt
     1021 caatacacag gtggaaacac catagggcaa tactttacac caagacacat tactcgtttt
     1081 atggcggata tttgtgagac aagcaaaaca gacttggtta tagaccccgc ctgtggaaca
     1141 ggtggctttt taatatctac tatccaaaga gcatatgatg ttttgggtat taagtatgaa
     1201 gacgctataa atatagtgaa agagaagtta ataggttacg aatccgaacc agtcacggcg
     1261 gctttgtgcg tagcaaacat gatattgcgc ggcgatggaa aaactggaat tagaaaagac
     1321 gatagtttta cagcagcaga ttttccaatt ggtaaatgcc aagttgcgtt aatgaatcca
     1381 cctttccccc acaaacaaac agatgtgcca tctcaacaat ttgtggaacg tgccttagaa
     1441 gcattagaaa atcgcggttt attagcagtt atcttaccaa gcagtctttt agtcaaaaag
     1501 gacattggcg aatggagaag aaccattctg cttaacaaca ctcttgaagc cgtttgccaa
     1561 ttacctgatg aactttttca gccttatgca agcgcaacaa cttcggttgt acttttacgc
     1621 aaaggagttc cgcatagtaa aagcaaaaaa actgtctttg ttcgtttgcg ccacgatgga
     1681 ttaactctga aaaagggaac tagaataccc agagcagacg gtcttaatga tataccgtcc
     1741 gcaacagaag caattctaaa taaacgtcaa gacgcagggt ttagcggtct cgccatagta
     1801 agtcaagatg atgaatggtc tgtgggggca tatattcctt ctggcgctcc gtctattgac
     1861 gaacttaaaa acagcgtaga cgaatcgttg cgtagattag tatcttttta tgtaaggtat
     1921 gctcaagaag ttgcaaccct ccgaagtagt gtaaaaaacg gagcggttaa tgtttcgcca
     1981 tatcgagaca tgttgagttc aatgcgtttg gacaatgcca agactatatc tacacaaaaa
     2041 aatactattg gtgagatttt cgatatttac tatggtcaaa aagagttgca tagtagagat
     2101 ggtataccta acggcgtatc attaattatt tctccaacag aacaatataa tggatgttat
     2161 ggatggcttg attttcctaa ccttatcaag ccgccctttg tcacagttgc gcaaactggt
     2221 tcaatcggag aaacttttgt tcaacttgaa ccatgcggag taaatgatga ctgtttaatt
     2281 ttattgccta aagaaggaat tgatttacct ttgtctagtt actttattgc ttctgctaca
     2341 attcgactag agaaatggcg gtttagttat ggtagaaaat taacacctgc tcgtatatct
     2401 gattttcaga tgactcattc tccacaatta gaagaatgga taaatgaaca aatccgtaat
     2461 tggaatcaaa tcattaataa atctataaaa gcatacgagt cttaagaaaa cgcagcctaa
     2521 caaagcgtgc acctgacgtg tgggattctg cggcattttc aagcattttt ctggcttcga
     2581 gtttttcctg ctcccaagca gaatccacgc ccgcccacac gcaggtaacg caaaccgttg
     2641 ggcgcttgtc attaaggctt ttcgcaaatc aataatttat gaaaaaatca cttaattcac
     2701 cccttgaaac tattcttgag tcaatagtta tccttgttgt ttgggttgta tttctcccaa
     2761 ttttctgggc tatgtctgaa gcaaatagcg agaataaagt tttgttctgg ctattcttat
     2821 ttttaggctt agtactaatt ttcttgcagt ggaaattctt acccattaga catattgagt
     2881 tcgatggaaa ctttatttat gtttcaaact attttaggaa aatagtcatt gcaatttcag
     2941 aaattgaaga
//
LOCUS       DAOQJE010000444         2949 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_22805_length_2949_cov_4.373186, whole genome shotgun sequence.
ACCESSION   DAOQJE010000444 DAOQJE010000000
VERSION     DAOQJE010000444.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2949)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2949)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2949
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22805_length_2949_cov_4.373186"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            202..1791
                     /locus_tag="PLT08_11540"
     CDS             202..1791
                     /locus_tag="PLT08_11540"
                     /inference="COORDINATES: protein motif:HMM:NF015210.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF262 domain-containing protein"
                     /protein_id="HNE05145.1"
                     /translation="MGLTTKTLGELLDYVDSERLKLPEIQREFVWTRQNIKLLFDSLY
                     RDMPIGQMLVWKPNQSIPKARGFSRKPNRRDPSKIDLFYGFLLDGQQRLTAISRIRDS
                     DEEYPLMVDLAAALDEKEDIFFWGTRREFNNPWCVMVSDVISPNFNISGYLNALRDEE
                     DFMVQDEEPIRKMLTKLQGVLNYHISIIEFESDDYAEATQLFIRFNSTGKKLAKSDLV
                     LAELATRIEGLTSKKMGKLIDEWKPNYQFTRPFLIQCLAAVHTHRMDIKKPEDVWGDS
                     TPKQIEKSWERTAEALDEVIEFLTGFIQWESSSWLPSFNALIPLVYINAYNRSFNAQE
                     KRLARKWLLLAGVHAHFSGSVQTELDRILKKLNSNVSVDQLWKITSKKLPKLRADNDL
                     QVSRRSGPLMSLFISMLRNENSRDWISGNPLNGTVLGKNAKLHVHHFFPRALLYKHNY
                     SSEWINAFANYTVLCSGTNWNISTEEPLTYLSRLKIHKNHLEAQCIPQDPSLWQVARY
                     EDFIVEREKLLTKRLNEYLDT"
     gene            2059..>2949
                     /locus_tag="PLT08_11545"
     CDS             2059..>2949
                     /locus_tag="PLT08_11545"
                     /inference="COORDINATES: protein motif:HMM:NF024197.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EcsC family protein"
                     /protein_id="HNE05146.1"
                     /translation="MNTTFSSDDENLKSQVSKELEEAKRLAKSFEFEKVKNGEWFITL
                     LKQVTKAYDRNARSAYFQKKYPGLSPDEIADILTSVTVRYATIAGAVAGVAASASEIA
                     SLGSAGMTATLFIGAIGAEMLYLSNIQMRLMLDMSVLYDLHLDPEDPEDILMVFGYAL
                     GVAPTELLGKGLQVATRAGTESGIKKVVTKGTREAIVKFAQRLGFKIGQKTIIKYAVP
                     VASAAVGSGYNYVTTKSVGQIAKLHFKNRGKVTDELRSVISKQNTYDLAIPAAAMFMA
                     QVDGNVSPKEKELYRAMLSRM"
BASE COUNT          944 a          603 c          629 g          773 t
ORIGIN      
        1 aagtcattcc gctacaggaa cgcatcgaaa ccctcaccga tgcatccgac ctcgaagaag
       61 cctacaacac cgaacgtcac ctactctatg ttgcatccac tcgtgcaaga gatcatttgt
      121 tgattacggg agtggatcct gcgagtgagt ttttggaaga tatgaaataa attaataaga
      181 tcatctgtag gagaatagaa aatgggatta acaacaaaaa cacttggtga attacttgac
      241 tatgttgact cagaacgact taagcttcct gaaattcagc gtgaatttgt ttggacacgt
      301 caaaacatca aacttctttt tgattcactt taccgcgata tgcctatcgg gcaaatgtta
      361 gtctggaagc caaaccaatc tataccgaaa gctcgcggtt tttcaagaaa gcctaaccga
      421 cgggacccat caaaaatcga tcttttttat ggttttttgt tagatgggca acaaaggttg
      481 actgcaatat cgcgtatccg cgactcagat gaagaatatc ctttgatggt tgaccttgcc
      541 gctgcactcg atgaaaagga agatatattt ttttggggga cccgaagaga gttcaataac
      601 ccctggtgtg tgatggtttc agatgtaatt tcgccaaatt tcaacatcag tggttatttg
      661 aatgctctta gagatgaaga agattttatg gttcaagacg aagaaccaat tcgtaagatg
      721 ttgaccaaac ttcaaggggt tttaaattat catataagca tcatagaatt tgaatcagat
      781 gattatgcag aagcgactca gctattcata aggttcaact ccacaggtaa aaagcttgcc
      841 aaaagcgatt tagttcttgc cgaacttgct actcgaattg agggtttgac ttctaagaaa
      901 atgggcaaac tcattgatga atggaaaccc aattatcaat tcactcgtcc attcttaata
      961 caatgcctgg cagccgtcca tactcatcga atggatataa aaaagccaga ggacgtctgg
     1021 ggagattcca cgccgaaaca aattgaaaaa tcatgggagc gaacagcaga ggcacttgat
     1081 gaagtcattg aatttctaac gggttttatt caatgggaat cttcaagttg gctaccctct
     1141 ttcaatgctc taatcccatt ggtttacata aatgcataca acaggtcgtt taatgcacaa
     1201 gaaaaacgct tggcgcgtaa atggttatta ttagcaggag tacatgcgca tttcagcggg
     1261 tcagttcaaa ccgaattaga tcgaattctt aaaaagctaa atagtaacgt ctctgttgat
     1321 caattatgga aaatcacatc taaaaaactt ccaaaattaa gagctgataa tgatttgcag
     1381 gtatcacgtc ggagtggacc tcttatgtca ctttttatat ccatgctccg caatgaaaat
     1441 tcaagggatt ggattagtgg caacccatta aatggaactg tgttgggtaa aaacgcaaaa
     1501 cttcatgtcc atcacttctt cccaagagct ttgctttaca aacacaatta ttcatcagaa
     1561 tggatcaatg cttttgctaa ttacacggtt ttgtgctcag gtactaactg gaatatatcc
     1621 actgaagagc cgctaactta cttatcacgt ctaaaaatac ataaaaatca tttggaagct
     1681 cagtgcatac ctcaagatcc atctctttgg caagtagctc gctatgagga ttttattgtt
     1741 gagcgagaaa agttgctaac aaaaagatta aacgaatacc tcgatactta gaaaaaaatc
     1801 aaatccgaga tcaaatacta aaccatcaag aatccctgca agttgaagcg aagaaatcca
     1861 agcccaggga agaacggggc aagtccactg tggaagggtt tgcgcaagtt acccggaatt
     1921 tcaacgatat cggcaagccc atggtggaac ttgcaacgac ggtgatcaaa tttttcaata
     1981 cactgcttgg gtaaagtctt ttgatcaagt agtaaagaaa gatatagtac gaatttagat
     2041 actacaagga ggtttacatt gaacaccaca ttctcatcag atgatgaaaa cctaaaatcc
     2101 caggtctcga aagaacttga agaagcgaaa agactggcaa aatcgtttga gtttgaaaaa
     2161 gtaaaaaatg gtgaatggtt catcaccctg ctcaaacagg tcaccaaagc atatgaccgc
     2221 aatgccagat ctgcttattt tcagaaaaaa tacccaggac tatcccctga cgaaattgca
     2281 gatatcctaa cctctgtaac agtcagatat gccaccattg ccggggcggt cgccggagtt
     2341 gcagcgtcag ccagtgaaat agcatccctc ggttcagcag gtatgacagc caccttgttt
     2401 atcggagcca tcggcgcaga aatgctttac ctctccaata ttcaaatgcg gcttatgttg
     2461 gacatgtcgg tgttatatga cttgcatttg gatccggagg atcctgaaga tatactcatg
     2521 gtattcggat atgcgcttgg cgtggctccc actgagttgc tcggtaaagg gctgcaagtt
     2581 gccacacgcg ctggaacaga aagcgggata aaaaaagtag tcaccaaagg aaccagagaa
     2641 gccatagtga aattcgcgca aaggctaggt ttcaaaattg ggcaaaaaac gattatcaag
     2701 tacgccgttc ctgtggcgtc tgctgcggta ggcagtggct acaactatgt aacgaccaag
     2761 tcagttgggc aaatcgctaa actccatttc aagaatcgcg gcaaagttac cgatgaactg
     2821 aggtcggtaa tctcaaagca aaacacctat gatttagcta tacctgctgc agctatgttc
     2881 atggcacaag ttgacggcaa tgtgtcgccc aaagagaagg agctatatcg ggctatgctt
     2941 tcgcgcatg
//
LOCUS       DAOQJE010000445         2933 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_23003_length_2933_cov_5.581654, whole genome shotgun sequence.
ACCESSION   DAOQJE010000445 DAOQJE010000000
VERSION     DAOQJE010000445.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2933)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2933)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2933
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23003_length_2933_cov_5.581654"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1075)
                     /locus_tag="PLT08_11550"
     CDS             complement(<1..1075)
                     /locus_tag="PLT08_11550"
                     /inference="COORDINATES: protein motif:HMM:NF017181.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE05147.1"
                     /translation="MSDILPFLKSIISISGVSGDEAPVADLVQKKWTPLVDEISQSRL
                     GSIHGLKQGTLKKSGSKAAASSRPSVMIATHMDAIGMMVTRIVDGFIYFTNVGGIDSR
                     VLPGTPVIVHASGTKEELYGVIVMPPGNLLPEGEGGGVVPMKYLFVDTGLFAGEVSKR
                     VRVGDRISFGTEPVEMSGGTVSGHTLDNRASVAALTVCLEELQSKSHIWDLWAVATVQ
                     EETSFGGSYSSTFQLRPTIAIALDMTFGKGAGASGYQTFPLGKGVTLGIGPSVHPFLY
                     QRFKEVAERIEIPIADDLMPEYSSTDADAMQLIAEGIPTMVVSIPQRYMHTAVELVAL
                     KDIQRAGRLMAEFVASLEADFLER"
     gene            1105..1452
                     /locus_tag="PLT08_11555"
     CDS             1105..1452
                     /locus_tag="PLT08_11555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE05148.1"
                     /translation="MVVGVGVLVIVAVMVGVFDAVGVNVLVGVKVRVVVGVNVNVLVL
                     GRKNVSVAVGVDVDVGVEVIVGVNVIVPVTIKGVLLAVAVFGVPVTVSVMDGVGVTSR
                     GLGAKERQTNPAQ"
     gene            1566..>2933
                     /locus_tag="PLT08_11560"
     CDS             1566..>2933
                     /locus_tag="PLT08_11560"
                     /inference="COORDINATES: protein motif:HMM:NF024867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMC family ATPase"
                     /protein_id="HNE05149.1"
                     /translation="MIPLHLRIAGFLSYRDPVDLDFNMVDLACISGHNGAGKSSLLDA
                     FTWSLFGEARGKGVDVINLSQDVKAAEVALTFLYEGNIYRVQRTLPRNKSTVLEFQVL
                     DRGSQTTDDGRWRPLTEKTTRDTQARIEQTLRLDYETFINASFFLQGKADQFTQKKAS
                     ERKAVLSAILGLEIWDTYKDRTAERRRELETEVTEIDGRIAEIDAELGEEDERRRRLS
                     ELEESLKQLTTAREAQESALDNIRRNAALINEQRKTVANAETQLKRVQDTLTSIEAKI
                     AVKENERAEYLDLLERAKDIESAYKAWQKARKELEAFDANATEFREHDEKRQPLLREI
                     EVEKAKLEQEGEELGKQYSVINDYVAMVESLESQLNTARESLKEAEEKIQERTKLEAQ
                     RNEARERQAGLKAENESLRREMDELKTRIDALKVAEGAECPLCGQPLSESHRKSTLKD
                     LEQTGK"
BASE COUNT          757 a          639 c          871 g          666 t
ORIGIN      
        1 ttctttcaag aaagtccgct tcgagcgaag cgacgaattc agccatcaaa cgacccgcac
       61 gttgaatatc tttcaacgcc accaactcca cggcagtgtg catgtaacgc tgcggaatgc
      121 tcaccaccat tgtgggtata ccttccgcga tcaactgcat cgcatcggca tcggtggagg
      181 agtattcggg catcaaatca tcggcgatgg gaatttcaat ccgttcggca acttctttga
      241 agcgctgata taaaaacggg tgaacgctgg gtccgatgcc taaggtcaca cctttgccaa
      301 gcgggaaggt ttggtatccg cttgcgcctg cgcctttgcc gaaggtcatg tcgagcgcga
      361 ttgcgatcgt cgggcgcagt tggaaggtgc tgctgtagga accaccgaag gacgtttctt
      421 cttgcacggt tgccactgcc cacaagtccc agatatgaga tttggattga agttcttcaa
      481 ggcagacggt gagcgcagcc acagaggcgc gattgtcgag cgtgtgtccg ctcacggttc
      541 ctccggacat ttcaactggt tctgtaccaa aagagattct gtcgcccaca cggactcgct
      601 tcgagacttc gcccgcgaat aaacctgtat caacgaacag atacttcatg ggcacaacgc
      661 cgcccccttc gccttcaggg agcaaattgc cgggcggcat gacgatgacg ccgtagagtt
      721 cttctttcgt ccccgaagcg tggacgataa cgggcgtacc gggcaacacg cgcgagtcga
      781 tgccgccgac gttggtgaaa taaataaagc catccacaat gcgggtgacc atcataccga
      841 tggcatccat gtgggtggcg atcatcaccg atgggcggga agaggctgcg gctttggatc
      901 cgcttttctt tagcgtaccc tgcttcagtc catgaatcga gccgaggcga ctttgggaga
      961 tctcatcaac cagtggagtc cacttttttt gaacgaggtc cgctacgggg gcttcatccc
     1021 cagaaacgcc agagatggag ataatcgatt ttaggaaggg gagaatatcg ctcatgaagt
     1081 tatggggtag gggatggagt gagtgtggtt gttggggttg gcgtattagt gatcgttgcc
     1141 gtaatggttg gcgtgttcga tgccgttggt gtaaatgtat tggtaggcgt gaaggtgagg
     1201 gttgtggtgg gagtaaatgt aaatgtgttg gtgctaggca ggaagaacgt gagcgtagcc
     1261 gtaggtgtgg atgttgatgt aggcgtggaa gtgattgttg gcgtgaatgt aattgtgccg
     1321 gttacgatca aaggtgtgct actggcggtg gctgtgttcg gggtgcccgt cacagtttct
     1381 gtgatggatg gggttggggt cacatcgcgc gggttgggag caaaggaaag acaaacgaac
     1441 ccggcacaat aacagggtat ggatgcaata atgattgcga gaagccgcat gcggcggcgt
     1501 gcttgaggtg tttcggcgtc tagcatagtg gttcgattat aatccatgca attgggtagc
     1561 atttaatgat tccacttcac cttcgtattg ctggtttcct ctcttatcgt gaccctgttg
     1621 acttggattt caacatggta gaccttgctt gtatttctgg gcataacggc gcgggcaagt
     1681 cttctttatt ggatgcgttt acatggtcat tgtttgggga ggcacgcggt aaaggggtgg
     1741 atgttatcaa cctaagccag gacgtaaaag ctgctgaagt tgccctcacg tttttatatg
     1801 aaggcaatat ttatcgcgtg cagcggactt tgccacgcaa taagagtacg gttttggagt
     1861 ttcaggtctt agaccgtggg tcacagacga ccgacgatgg acggtggcgt ccgctgacgg
     1921 agaaaacgac ccgtgatacg caagcccgca ttgaacaaac cttacgcctc gattatgaga
     1981 cgtttatcaa cgcttcgttc tttttgcaag gcaaagccga ccaattcacg cagaagaaag
     2041 ccagcgagcg aaaggcagtg ctaagcgcca ttctcgggtt ggaaatttgg gatacctaca
     2101 aagaccgaac ggcagaacgt cgtcgtgaat tggaaaccga agtcactgaa attgatggtc
     2161 gcattgcgga gatcgacgcg gaattagggg aagaagacga acgccgacgc cgtttaagcg
     2221 aattagaaga atcactgaag cagttaacga cggcgcgcga agcacaagaa tccgcactgg
     2281 acaatatccg ccgaaatgca gctttgatca atgagcagag aaaaacagtt gcgaacgccg
     2341 aaacgcagtt gaaacgggta caagatacgc ttacttccat tgaggcgaag atcgctgtca
     2401 aggaaaatga acgcgccgag tatttggatt tgttagaacg agcaaaggat attgagtccg
     2461 cgtataaggc gtggcaaaaa gcgcgtaaag agttggaagc gttcgatgcc aacgcaacag
     2521 agttccgcga acatgatgag aaacgtcaac ccttgttgcg cgaaattgaa gtagagaagg
     2581 cgaagttgga gcaagaaggg gaggaactcg gtaaacagta ttcagtaatc aatgactatg
     2641 tggcaatggt tgaaagccta gaaagtcaat tgaatactgc gcgagagtcg ctaaaagaag
     2701 cagaagaaaa aattcaagaa cgcactaaat tggaagctca gcgcaacgaa gcgcgggagc
     2761 gacaggcagg cttgaaagcg gagaatgaat ctctgcggcg ggagatggat gaactcaaga
     2821 cccgcatcga tgccttgaaa gttgcggaag gcgcggaatg tccgctgtgt gggcagcctt
     2881 tgagcgaaag ccatcgtaaa tctactctga aagatttgga gcagaccggc aaa
//
LOCUS       DAOQJE010000446         2933 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_23004_length_2933_cov_5.429812, whole genome shotgun sequence.
ACCESSION   DAOQJE010000446 DAOQJE010000000
VERSION     DAOQJE010000446.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2933)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2933)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2933
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23004_length_2933_cov_5.429812"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(92..592)
                     /gene="trxA"
                     /locus_tag="PLT08_11565"
     CDS             complement(92..592)
                     /gene="trxA"
                     /locus_tag="PLT08_11565"
                     /inference="COORDINATES: protein motif:HMM:TIGR01068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HNE05150.1"
                     /translation="MNKQEFQEKINSSEKPLVVDFWATWCAPCMMTKPILEKLGKEYA
                     EDVEFLPINADESRDVLEDYRILGIPTVLAFKGGKEVARVTGAQNEAGYRAIFESMAK
                     GVKVKIPMTQIDRMLRLGAGGLLVMYGLPDRNWIVVAIGAVVAFLGIYDRCPIWNALT
                     KPFQRK"
     gene            complement(645..845)
                     /locus_tag="PLT08_11570"
     CDS             complement(645..845)
                     /locus_tag="PLT08_11570"
                     /inference="COORDINATES: protein motif:HMM:NF022577.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2892 domain-containing protein"
                     /protein_id="HNE05151.1"
                     /translation="MKRNLSDVDRIIRVVLAAIFAYLYFGGIVTGTFGIVLVVLAVVF
                     ALTAVVAFCPLYAPFKLSTYKK"
     gene            complement(917..1591)
                     /locus_tag="PLT08_11575"
     CDS             complement(917..1591)
                     /locus_tag="PLT08_11575"
                     /inference="COORDINATES: protein motif:HMM:NF012257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HNE05152.1"
                     /translation="MIDPKQFERIVQSMPFLKNADSGFVKEFQRSAFLAQIPAGHDVF
                     LEGDRVEATALLISGVVRVYKIGETGREITLYRFGNGSSCILTANAILSRNTFPAIAT
                     VEQDAEAVMIPADVFRDWVKRYDLWRDFVFDLLSQRLSTVMAVVDEVVFHRMDRRVAN
                     LLLKRGKEENPIRITHQEIASDLGSSREVISRLLEDFISEGSVRSGRGIIEVLDFKTL
                     EDRSLM"
     gene            complement(1595..2446)
                     /locus_tag="PLT08_11580"
     CDS             complement(1595..2446)
                     /locus_tag="PLT08_11580"
                     /inference="COORDINATES: protein motif:HMM:NF014679.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005504 - fatty acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HNE05153.1"
                     /translation="MSKFAIVTDSTSYIPTDVAQKHGITIAPQVLIWDNQTYRDGIDI
                     QPTDFYSRLKTAKSMPSTSQVSPATMQEIFQGLVEKGTSVLGIFISSKLSGTLQSAIQ
                     AKDMMGSAGEKVTLVDSRSTAMGLGFQAIIAARAAEAGASIEEAASQASSAHERTGVF
                     FAVDTLEFLHRGGRIGGAQRFIGSALNLKPILAVKEGKVEGIERIRTKSKAHERVLEL
                     VAEQVKGKSNVRIATLHANSADDAKSLLDRAATELSPVETLFTEVSPVVGTHAGPGTV
                     GLAYIHD"
     gene            2586..>2933
                     /locus_tag="PLT08_11585"
     CDS             2586..>2933
                     /locus_tag="PLT08_11585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HNE05154.1"
                     /translation="MAFNPINWLLGLFSLDIAIDLGTANTLVHVRGKGIVINEPSWVA
                     VDKKQRQPLAIGLEAKEMVGRTPANVVVVRPIRDGVIAEFDVTRVMLEYFIGKVHEQS
                     VVPLPRPRVIVGLP"
BASE COUNT          755 a          715 c          683 g          780 t
ORIGIN      
        1 atgcatcata tgacgagatt atacccgcca ctctcattgg gacgcaaacg gctccggacg
       61 aagccggagc ctgtttcctg actattgaac tttattttcg ctggaagggt ttcgtcagtg
      121 cattccaaat tgggcaacgg tcgtaaatgc ccaaaaaagc aacaaccgcg ccaatcgcta
      181 caacgatcca atttctgtca ggcaaaccat acatgaccag caaaccgcca gcacccaaac
      241 ggagcatacg gtcaatttgg gtcatcggga tttttacttt cactcccttt gccatggatt
      301 caaagatggc acgatagccc gcctcgttct gagcgccagt gactcgtgct acttcttttc
      361 cacccttaaa tgctagcaca gtgggaatcc cgagaatacg atagtcttcc aatacatccc
      421 gagactcatc tgcattgatg ggtagaaatt ccacatcctc ggcatattct ttgccgagtt
      481 tttctagaat gggtttggtc atcatgcatg gagcgcacca tgttgcccaa aaatccacta
      541 cgaggggttt ttcgcttgaa ttgattttct cttgaaattc ttgtttgttc ataatttaaa
      601 ggataggagc gggcaaaaca ttgcccgctc ccaaatgttt tgaattactt tttgtaggtg
      661 ctaagtttga aaggtgcata caacgggcag aatgcaacca cagcggtgag ggcaaatacg
      721 acagcaagca cgaccaagac aataccgaac gttccagtaa cgataccacc aaagtacaag
      781 taggcaaaaa ttgcagccag caccacgcgg ataattcggt cgacatcaga caaatttcgt
      841 ttcattttct agtttctcct tttggttatt gttaaccgta gtataggaga aactcccgaa
      901 cctgtctgtg actatgtcac ataagcgaac gatcttccaa cgtcttaaaa tctagaactt
      961 cgatgattcc acgccctgag cggacgctgc cctcactgat gaaatcttcg agtaagcggc
     1021 tgatcacttc tctggaactt cccagatctg aagcgatttc ctgatgcgtg atgcgaatag
     1081 ggttttcttc ctttccacgc ttaaggagta aattcgccac tcggcggtcc atacggtgga
     1141 aaacaacttc gtcaactaca gccatcactg tagaaaggcg ttgagataac aggtcaaaaa
     1201 caaagtcacg ccacaagtca tagcgcttga cccaatctcg aaatacatcg gctggaatca
     1261 tcacagcttc tgcatcttgc tcaaccgtgg caatcgctgg gaatgtgttt cgactcaaga
     1321 ttgcattcgc ggtcagaata caggatgaac cgttaccaaa gcgatagagc gtaatctcgc
     1381 gccctgtttc accaatttta taaacacgta ccacgccaga gatcagcaag gcagtggctt
     1441 caacacgatc accttcaaga aagacatcgt gccctgccgg aatctgcgca agaaacgcag
     1501 acctttggaa ttcttttaca aatcccgaat ctgcgttctt caaaaaaggc atggattgaa
     1561 cgatacgttc aaactgcttt gggtcgatca tggattaatc gtgaatgtaa gcgagcccta
     1621 cagtgccagg tccggcgtgg gttccaacca ctgggctaac ttcggtaaat agggtttcca
     1681 ccggagagag ttcagtcgca gcgcggtcta acaagctttt ggcatcgtct gccgagttcg
     1741 catggagagt cgcaatgcga acgtttgatt ttcctttgac ctgttcggca accaattcaa
     1801 gcacacgttc atgagctttg ctcttggtgc gaatgcgttc gatgccttct actttgcctt
     1861 ctttgactgc aaggatcggc ttgaggttca gcgcagagcc gatgaagcgt tgtgcgcctc
     1921 caatacgccc accacggtgc aagaattcaa gtgtatctac tgcaaagaac actcctgtgc
     1981 gctcatgtgc gctagatgcc tgagacgcag cttcttcaat gcttgcgcca gcttctgcgg
     2041 cgcgagcagc aataattgcc tgaaacccaa gtcccattgc agtagagcgg ctgtcaacta
     2101 aggttacttt ttcgccagcg cttcccatca tatccttggc ttgaatggca gattgcaatg
     2161 tgcctgataa ttttgaagag atgaagatgc caagcacaga agttcctttt tcgaccaacc
     2221 cctgaaatat ctcctgcata gtggcaggag aaacctgcga agttgacggc atcgacttgg
     2281 cggtcttgag gcggctgtag aagtcggtgg gctgaatatc aataccgtcg cggtatgttt
     2341 ggttgtccca aatgagaact tgtggggcaa tggtaattcc atgtttctgg gcaacatcgg
     2401 ttgggatata agaagtgcta tcggtaacaa tggcaaactt tgacatttct cttcctccac
     2461 ggggaaaaat tttcctgatt ttaccccgtt tgggtaacag aattaaaaaa tattcggact
     2521 catcctcatg gtataatggc tcggcagttt tgcttagacc cattctcgta tcataggagc
     2581 cgattttggc cttcaaccca attaactggc tgttaggcct tttttctttg gatattgcca
     2641 ttgaccttgg caccgcaaat actcttgtcc atgttcgtgg caagggaatt gtgatcaatg
     2701 aaccatcttg ggttgccgtt gataaaaagc aacgtcagcc acttgcgatt ggtcttgaag
     2761 ccaaggaaat ggtggggcgt acccccgcaa atgtagtggt ggttcgtcct attcgtgatg
     2821 gggtgattgc tgagtttgat gtaacccgcg tgatgctcga gtactttatt ggaaaagtac
     2881 atgaacagag tgtggtccct ttaccacgtc cgcgcgtgat cgttggtttg ccc
//
LOCUS       DAOQJE010000447         2929 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_23054_length_2929_cov_5.017397, whole genome shotgun sequence.
ACCESSION   DAOQJE010000447 DAOQJE010000000
VERSION     DAOQJE010000447.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2929)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2929)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2929
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23054_length_2929_cov_5.017397"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(84..1571)
                     /gene="accC"
                     /locus_tag="PLT08_11590"
     CDS             complement(84..1571)
                     /gene="accC"
                     /locus_tag="PLT08_11590"
                     /EC_number="6.3.4.14"
                     /inference="COORDINATES: protein motif:HMM:NF006367.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0004075 - biotin carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HNE05155.1"
                     /translation="MLTKILIANRGEIAIRIMRACRELGIRTVAVYSEADKNAQHVQF
                     ADEAILLGGAAPKESYLNVDALLRATRDSQADAIHPGYGFLSENASFAAAVESANLTF
                     IGPSADSIRAMGDKAESKILMKKAGVPTVPGYEGLESYDDFKNAAKEIGYPVLVKAAA
                     GGGGKGMRVVNAESELNESIELARREALNAFGDERLLIEKYLAEAHHIEIQVFGDKHG
                     NLVHLFERECSVQRRHQKIIEESPSPLLTPELRAKMGEAAVNAAKAVNYYNAGTVEFI
                     FDPKTQQYYFLEMNTRLQVEHPVTELVTGLDLVHWQIRIAAGEKFPFTQSDLHQHGHA
                     IECRVYAEDPSTGFLPSTGKLLQFIQPQGPGIRVDSGFAAGDEVTHFYDPLLAKLIVY
                     AGSREDAITRMKNALNDFIIHGVVTNVDYMHAVLETDDFKQGKVTTRWVETKFGEWTV
                     PQPELHALIAAALADVVFVGGKAQPAVSNETDPFNPWKGTGSYRN"
     gene            complement(1561..2547)
                     /locus_tag="PLT08_11595"
     CDS             complement(1561..2547)
                     /locus_tag="PLT08_11595"
                     /inference="COORDINATES: protein motif:HMM:NF013530.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HNE05156.1"
                     /translation="MKILIIGGTRFIGRHLVTSARARGHEVTLFNRGKSNPGLFRRIQ
                     NIRGDREKDLDQLSGQWDAVIDTCGYLPRVVRLSAEALKEKVSRYVFISSVSVYANFS
                     KVGIKESDAVGTLADESVEEITGETYGPLKALCEKAVQDVYGARSLIIRPGLIVGPHD
                     PTDRFTYWPLRIAKGGDVLTPDRPDAMAQFIDARDLADFIIRLIDQNVSGTFNAVGYP
                     VTLNTIFETCKRISKSNANFKWAPIDFLEKNNVAPWSDMPIWVPEVGEDAGVSQVDVS
                     KAVAAGLTFSPLTDTVKAIYDWEFERPAEYEMKAGLKPEREAELLKLLNHAH"
     gene            complement(2544..>2929)
                     /locus_tag="PLT08_11600"
     CDS             complement(2544..>2929)
                     /locus_tag="PLT08_11600"
                     /inference="COORDINATES: protein motif:HMM:NF019247.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1579 family protein"
                     /protein_id="HNE05157.1"
                     /translation="ANEAPIVGTIQIILDGRYALFLYQSSIEGETQHGMFTFGYNTQL
                     DRYETSWVDSFHTNTSIMFCTGNEIENGFSVLGSYPDPSGGPDWGWRTDVEVRDHELV
                     ITAYNIMPEGAEAKATEIILKKVNA"
BASE COUNT          668 a          717 c          799 g          745 t
ORIGIN      
        1 gttttcattt tgttcaccgt aggggcgacc cttcattgtt tatgaaggtg ccttgctttc
       61 acagggcggg ttactcctac aaattaattc ctataactcc ccgtcccttt ccacgggttg
      121 aacgggtcgg tttcattgga aacggcaggt tgggctttgc cgccaacaaa gaccacatct
      181 gcaagggcgg cagcgatgag ggcgtggagt tcgggttgtg gcacagtcca ctcgccgaat
      241 ttggtttcga cccagcgtgt ggtgactttg ccttgtttga agtcatccgt ttcgagaacg
      301 gcatgcatgt agtctacatt cgtcaccaca ccgtgaatga tgaaatcgtt gagcgcgttc
      361 ttcatacgtg tgatggcatc ttcgcgactg ccagcataga cgatgagctt cgccagcagt
      421 ggatcataaa aatgggtgac ctcatcgcca gccgcaaacc cagagtccac gcgaatgccg
      481 gggccttgcg gttggatgaa ttgcagcagt ttgccggtgc taggcaaaaa gccagtggat
      541 ggatcttcgg catagacgcg gcactcaatg gcgtgaccat gttggtgcag gtcgctttgg
      601 gtgaagggga atttttctcc cgcagcgata cgaatttgcc agtggacaag gtcaagcccg
      661 gtgacgagtt cggtgacggg atgctccact tgcaggcggg tgttcatttc gaggaaataa
      721 tattgctggg tcttcgggtc gaagatgaat tcgacagtgc ctgcgttgta ataattaaca
      781 gctttagcag cattgaccgc ggcttcaccc atcttggcgc gcagttcggg ggtgagcaat
      841 ggggaagggg attcttcgat gatcttttgg tgacgtctct gcaccgagca ttcacgttcg
      901 aagagatgca cgaggttgcc gtgtttgtcg ccaaagactt gaatttcaat atgatgtgct
      961 tcagcgagat atttttcaat gagcaggcgt tcgtcaccga aagcattgag ggcttcgcgg
     1021 cgtgcgagtt caatagactc attcaactcg ctttcggcat tgaccacgcg catgcctttc
     1081 ccgcctccgc ccgctgcggc tttgacgagg acagggtagc caatttcttt ggcggcgttt
     1141 ttgaagtcgt cgtaactttc taagccttcg taacctggaa cagtgggcac accggctttc
     1201 ttcatcaata tctttgattc agctttatcg cccatggcac ggatcgagtc ggctgaggga
     1261 ccgataaagg ttaggttcgc tgattccact gcggcggcga aagaggcgtt ctctgagagg
     1321 aagccatagc ctgggtgaat ggcatcggct tgagagtcac gagtggctcg cagtaaggca
     1381 tccacattca aataagattc tttcggtgct gcccccccaa gcaagatggc ttcatcggcg
     1441 aattgcacgt gttgtgcgtt cttatccgct tcggaataga cggcgacagt tctaatgcca
     1501 agctcacgac aggcacgcat gatacgaatg gcgatctcgc cgcgattggc gataaggatt
     1561 ttagtgagca tggttgagta atttaagtaa ctcggcttca cgctcaggct tcaagccagc
     1621 tttcatttca tactctgcgg ggcgttcaaa ctcccagtcg taaatggctt tgacggtatc
     1681 tgtgagcggc gagaaggtaa gcccggctgc taccgctttc gaaacatcca cctgcgaaac
     1741 cccggcatct tcgccgacct ctggaaccca gatgggcata tcgctccatg gggcaacgtt
     1801 gttcttttca agaaaatcta taggcgccca tttgaaattg gcgttgctct tactaatccg
     1861 tttacaggtt tcaaaaatag tattgagcgt gacgggatac ccaacagcat tgaaggtgcc
     1921 cgaaacattt tggtcaatga gccggatgat gaaatcggct aggtcgcgcg cgtcaataaa
     1981 ttgagccatt gcatcggggc ggtctggagt gagcacatct ccacctttgg cgatgcgtaa
     2041 aggccaatag gtgaaacggt cagtggggtc atgcggaccc acaatgagcc cagggcgaat
     2101 aatcagtgag cgggcaccat atacatcttg cactgctttt tcgcacaaag cttttagagg
     2161 accataggtt tcgccagtga tctcttccac gctttcatcg gcaagggtgc caacggcatc
     2221 gctttctttg atgccaacct tgctaaagtt cgcatacacc gaaacgctgg aaataaatac
     2281 ataacggctg accttttctt tcagcgcttc agcagagagg cgcacgacgc gcggcaggta
     2341 gccgcaggta tcaatgaccg catcccactg ccccgataac tgatccaaat ctttttctcg
     2401 atctccccga atgttttgga ttcttcgaaa aagacccggg ttgctttttc cgcgattgaa
     2461 gagcgtgact tcatgtccgc gggcacgggc agaagtgacg agatgacgtc cgataaaacg
     2521 agtcccgccg ataataagga tcttcatgca ttcacctttt taagaatgat ttcagttgcc
     2581 tttgcttccg cgccttcagg catgatgttg tatgctgtaa taacaagttc gtgatctcgt
     2641 acttcgacgt ctgtgcgcca accccaatcg ggtccgccgg aaggatcggg ataggagcca
     2701 agcacagaga acccattctc gatttcattg cccgtgcaga acatgatgga cgtattggtg
     2761 tggaatgagt cgacccagct ggtttcatag cggtcgagct gggtgttgta cccgaaggtg
     2821 aacatgccat gctgagtttc gccttcgata gaggattgat ataggaaaag ggcataacgt
     2881 ccatcgagga tgatttgaat ggttccaacg atgggagctt cgttagcca
//
LOCUS       DAOQJE010000448         2928 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_23066_length_2928_cov_6.627567, whole genome shotgun sequence.
ACCESSION   DAOQJE010000448 DAOQJE010000000
VERSION     DAOQJE010000448.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2928)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2928)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2928
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23066_length_2928_cov_6.627567"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(27..617)
                     /locus_tag="PLT08_11605"
     CDS             complement(27..617)
                     /locus_tag="PLT08_11605"
                     /EC_number="2.7.7.77"
                     /inference="COORDINATES: protein motif:HMM:NF024214.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0061603 - molybdenum cofactor
                     guanylyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor guanylyltransferase"
                     /protein_id="HNE05158.1"
                     /translation="MLSVVIQAGGQSSRMGEDKALKPFLGKPLIQRVIDRLSPIADEM
                     IVTTNRPADYAFLNQRLIADLKPGRGSLGGLYTAIASARHPFVAVVACDMPFASKSFF
                     ESAHRLLVKEEADVLIAKTEAGYEPFHALYRRETCLPAIEAAIEADQWKAVAWISQVR
                     TRVLSLEELKAFDPSGLCFWNVNTPEEFEKAEQLAK"
     gene            636..1748
                     /gene="amrS"
                     /locus_tag="PLT08_11610"
     CDS             636..1748
                     /gene="amrS"
                     /locus_tag="PLT08_11610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system radical SAM enzyme"
                     /protein_id="HNE05159.1"
                     /translation="MNALAKRLDALTVEGELYEKLADDSVRCFACGHRCLIREGKRGI
                     CQVRFNTGGRLMVPHGYVAALQSDPIEKKPFSHVLPGSNALTFGMLGCDYHCPYCQNW
                     LTSQAMRDPASDVSAQFIRELTPEDVLSYARRTNASAVISSYNEPLITSEWAVEIFKV
                     AKENGLMCAYVSNGNNTPEAMDYLVPYLSAYKVDLKCMSDKNYRKLGGTLQHTLDGIK
                     RARDHGLWVEVVTLVIPGFNDSNEELWDAARFLAEVSKDIPWHVTAFHKDYKMTEPAN
                     TDARTLIRAAEIGREAGLKFVYAGNLPGTVGEYEDTACPRCSYRLIKRRGYVIKEYHL
                     TDEGKCPKCGESMPGLWHKDPSRVGLNGSGFPMALQ"
     gene            1820..>2928
                     /locus_tag="PLT08_11615"
     CDS             1820..>2928
                     /locus_tag="PLT08_11615"
                     /inference="COORDINATES: protein motif:HMM:NF012706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HNE05160.1"
                     /translation="MKRNLFSLLSLLVLASLVLAACGSAEATTAPEATEAATEAAATE
                     AATEAPAAEATPTPIPDVSIATFDGTSLAVPAEECNGDYIGLIQSVVAVDASTVVFTL
                     CRPDPAFLSKIAFSPFAIYPSEWLEATAGDTTRTSEGLEKPVGTGPYMVSEWARGDSV
                     TFVANPDYWGEQPAASTLVMRWSAESAARLLELQSGTVDGIDNLGPDDFAVVEADSSL
                     QVAKRPALNTFYIGMTNTFAPFDNQKVRQAIAMGIDRQRIVDTFYPAGSEVATHFTPC
                     AIPNGCAGDAWYDFNVDAAKALLAEAGFADGFSTKLYYRDVVRGYLPQVGNVAQDIQA
                     QLKENLNIDAEIVVMESGAFIEASSAGTLDGLYLLG"
BASE COUNT          659 a          753 c          785 g          731 t
ORIGIN      
        1 aaaaagtagg tcacgcattg cgtgacctac tttgctaatt gctcggcttt ctcaaattct
       61 tcgggggtgt tcacgttcca aaaacataag cccgaggggt caaaggcttt tagttcttca
      121 agcgataaga ctcgtgtgcg gacttgtgaa atccacgcca cagctttcca ttgatcggct
      181 tcaatggcgg cttcaatagc gggcagacag gtctcacggc gatataaagc atggaacggt
      241 tcatagcctg cttctgtttt tgcgatgaga acatccgcct cttccttaac gaggagcctg
      301 tgtgcgcttt caaagaagct tttactggcg aaaggcatat cgcatgccac caccgccacg
      361 aatggatggc gggcagatgc gatggcagtg tagagtccgc ctaaggagcc gcgacccggt
      421 ttgaggtccg cgatgaggcg ctgatttaga aaagcatagt ctgcggggcg attggtcgtc
      481 acaatcattt catcggcaat gggagagagc ctatcgatca cacgttggat caatggcttg
      541 ccaagaaaag gcttgagcgc cttatcttcg cccatccgag aagactgccc gcctgcttgt
      601 attacaaccg ataacatgag tatattatag gtgatatgaa tgctctggca aaacggcttg
      661 atgctctgac cgtagaaggt gagttgtacg aaaaacttgc cgatgattct gtgcgttgtt
      721 tcgcatgcgg acatcgctgc ctgatccgcg aaggcaagcg gggaatttgt caggtacgct
      781 tcaacacagg tggcaggtta atggtgccgc atgggtatgt ggcggcattg caaagtgacc
      841 caattgagaa gaagcctttt tcgcatgtac tgcccggttc caatgccttg accttcggta
      901 tgcttggctg tgattatcac tgcccatact gccagaactg gctcacttcc caagccatgc
      961 gagacccggc ttcagatgtt tctgctcaat tcattcgcga gttgactcca gaagatgtgc
     1021 tgagttatgc gcggcggacg aatgcttcgg cggttatttc ttcctataat gagccgctta
     1081 tcaccagtga atgggcagta gagattttca aagttgctaa agaaaatggg ttgatgtgcg
     1141 cgtatgtctc gaatggtaat aacacgcccg aagcgatgga ttatctggtg ccatatcttt
     1201 ctgcctataa agttgacctg aagtgcatga gcgataagaa ttatcgcaaa ctcggtggca
     1261 ccttgcagca tactttagat ggaatcaagc gtgccagaga ccacggttta tgggtagaag
     1321 tggtgacctt agtcattccg ggctttaatg actcaaatga agagttgtgg gatgcagcac
     1381 ggttcctggc tgaggtttca aaagatattc cttggcatgt gacggctttc cataaagatt
     1441 acaagatgac cgagccagcc aacaccgatg cccggacgct catccgtgcg gcggagattg
     1501 ggcgtgaggc ggggctgaag ttcgtctatg cgggtaactt gccgggaacg gtgggggagt
     1561 atgaagatac tgcgtgtcca cgatgcagtt accggctgat aaagaggcgg ggatatgtca
     1621 tcaaggaata tcaccttaca gacgagggaa aatgcccgaa atgtggcgaa tccatgcctg
     1681 gtttatggca taaagaccct tcgcgagtag ggttaaatgg aagtggattt ccgatggctt
     1741 tgcaataggt gtataattcc gcaacatttt gcgcggcaaa atgaataaat cccctttctc
     1801 acatggagga gaaacctgta tgaaacgaaa tctgttctct ttgctttcgt tattggtgct
     1861 cgcgagcctt gttctcgcgg cttgtggtag cgctgaagcc acaactgcac cagaagcgac
     1921 ggaagctgcg actgaagctg cagctaccga agcagcgaca gaagcacctg ctgctgaagc
     1981 cacccccacc ccgatccccg atgtttccat cgctaccttt gatggcactt cccttgctgt
     2041 gcctgctgaa gaatgcaatg gcgattacat tggtttgatc cagtctgtgg ttgcggttga
     2101 cgcctcaacc gtggtcttca cactctgccg cccagatccc gccttcctct ccaagatcgc
     2161 gttcagcccg tttgcgatct atccttccga atggcttgaa gccaccgctg gcgacaccac
     2221 ccgcaccagc gaaggtctcg aaaagcctgt cggtactggt ccttacatgg tgagcgaatg
     2281 ggctcgcggt gatagcgtga ccttcgttgc caaccccgat tactggggtg aacagcctgc
     2341 cgcctccact ctcgtgatgc gctggtctgc tgaatctgct gctcgcttgc tcgaactgca
     2401 gtctggcacc gtggacggta tcgataacct cggtcctgat gatttcgctg tggtcgaagc
     2461 ggattccagc ctccaggttg cgaagcgccc cgccctcaac accttctaca tcggtatgac
     2521 caataccttc gctccgtttg acaaccagaa ggttcgtcag gcgatcgcca tgggtattga
     2581 ccgccagcgc attgtagata ccttctaccc cgccggctcc gaagttgcca cccacttcac
     2641 cccctgcgcc atcccgaatg gttgtgctgg tgatgcttgg tatgacttca atgtcgatgc
     2701 cgccaaggct cttctcgccg aagctggctt cgccgatggc ttctccacca agttgtacta
     2761 tcgtgacgta gtccgcggtt acttgcccca ggttggcaat gtggctcagg acatccaggc
     2821 tcagttgaag gaaaacctca acatcgatgc cgaaatcgtt gtgatggaat ccggtgcttt
     2881 cattgaagcc tcctccgctg gtaccctcga tggtttgtac ctcctcgg
//
LOCUS       DAOQJE010000449         2927 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_23094_length_2927_cov_3.860724, whole genome shotgun sequence.
ACCESSION   DAOQJE010000449 DAOQJE010000000
VERSION     DAOQJE010000449.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2927)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2927)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2927
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23094_length_2927_cov_3.860724"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..756
                     /locus_tag="PLT08_11620"
     CDS             <1..756
                     /locus_tag="PLT08_11620"
                     /inference="COORDINATES: protein motif:HMM:NF013884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HNE05161.1"
                     /translation="AEQVLSFNGVSWFLSTLFFIYLLIPFVLFALQKMKIPQNIWSMI
                     FLAVACFFIVILVGYLLRHDLENLSAGWWFIYISPYMRFFDFMIGLLTGLITLRFREL
                     NLLSKIGSNLFTFAELLVLFSFGYMVYYTQFLSAKYTFLFYSSFFLPFSVLLVFVFSF
                     QKGHVSTVLSNKLLVYLGSLSFTMFMIHSLFISGMVAYFRTTVMLNIDTYLDGHRMTS
                     QLLLLFVIICVSDVVVRYYEAPLRQKIMSFFIL"
     gene            799..1797
                     /locus_tag="PLT08_11625"
     CDS             799..1797
                     /locus_tag="PLT08_11625"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HNE05162.1"
                     /translation="MPAVSIIIITWNSKKFLSDCIDKLNVQSFRDFEVILIDNGSVFD
                     MKPSIHEFSSFIQINAHFLETNLGFAVANNIGAQLANGEWIILLNADAYPEPDWLENL
                     LKAAEQNPQYNFFSSRQIQYNDPHLLDGAGDEYHVSGLAWRRFYNHVEKEYGLEPEEV
                     FGACAAAAMYRRSDFLKVGGFDEDYFSYFEDVDLSFRLRLAGGRCLYVPNAVVHHVGS
                     ASTGKLSDFVVYYGHRNLVWTFFKDMPGILFWVYLPVHLLMNLFFFFSFVFKGKGSAI
                     LRSKWDAFRMLPRILKKRKQIQSSRRISYKDILVFMRKRTLDPYWASRQRKLASNR"
     gene            1801..2565
                     /locus_tag="PLT08_11630"
     CDS             1801..2565
                     /locus_tag="PLT08_11630"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNE05163.1"
                     /translation="MTSSLITLSIVSHGDSQKISNVLASLLNHEQNTASRFQLILTDN
                     LKDNLPDTNPAPWKSLHMLRNDQPLGFAENHNRAFKLARGTYFAILNPDLIFEQPVFA
                     SLLTSLQTHNADLIAPTIVDETGTLQDSFRSLPTPIELIQRRMPGYTFQPPLPDADGM
                     IRPDWIAGMFWLLRADTYRQLGGMDKGYRLYFEDVDFCTRARLQGMNLLVDSNVKVRH
                     DARRSSRVKLYYLALHTRSALRFFTSPVYRKIRKIR"
     gene            2657..>2927
                     /locus_tag="PLT08_11635"
     CDS             2657..>2927
                     /locus_tag="PLT08_11635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE05164.1"
                     /translation="MSDPLFFGITSLIMSLALGIPAAILAQRFNLMDVPGSAPHKQHA
                     RPTPLAGGLLILLVFCLTFLLKPSYNHEIIAVLLAGLTIFFFGLWD"
BASE COUNT          712 a          654 c          584 g          977 t
ORIGIN      
        1 gcagaacaag tactatcatt taatggtgtg tcctggttct tgtcaacgct cttttttata
       61 tatttactga ttccctttgt attatttgct ctccaaaaga tgaaaatccc ccaaaacatt
      121 tggagcatga tttttcttgc ggtagcttgt ttctttattg ttattctggt tggctatttg
      181 ttgcgtcacg atttggagaa cctgtcagcg ggctggtggt ttatttatat atctccatat
      241 atgcgttttt ttgattttat gatcggtttg ttaacaggtt tgataacttt gcggtttcgc
      301 gagttgaatt tgctttcaaa gataggatca aatcttttta catttgccga attattggtg
      361 ctgttttcat tcggatacat ggtttattac acacagtttc tttctgcaaa atacaccttc
      421 ctgttttata gttcattctt tcttcccttc tctgtattgc ttgtatttgt tttttcgttt
      481 cagaaaggtc atgtttccac tgttctttct aataaattgt tagtgtactt aggcagctta
      541 agctttacaa tgtttatgat ccattcctta ttcattagtg gaatggttgc ttatttccga
      601 acgacagtta tgttaaacat agatacgtat ctcgatggac atcgtatgac ttcgcaactt
      661 ttacttctat ttgttattat ctgcgtttct gatgtggttg taaggtatta tgaagcacct
      721 cttcgtcaga agataatgag tttttttatc ctataaacaa taactgagtg atgggttttt
      781 atcaattaaa gagattcaat gccggctgtt tcgataatta tcattacatg gaatagtaaa
      841 aaatttcttt ctgattgcat tgataagttg aatgtgcaat cttttcgtga ttttgaggta
      901 atccttattg ataatggctc agtatttgat atgaagcctt ctatccatga gttttcatcg
      961 tttattcaaa taaatgctca ctttctagaa accaatctcg gcttcgccgt agccaacaac
     1021 atcggcgcac aacttgccaa tggcgaatgg attattctgc tcaatgccga tgcctacccg
     1081 gaaccagatt ggctcgaaaa tcttctcaag gctgcagaac agaacccaca atacaacttc
     1141 ttttcgtccc ggcagatcca atataacgac ccgcatctct tggatggagc aggggatgaa
     1201 taccatgtca gcggactggc gtggaggcgt ttttacaatc atgtcgaaaa agaatatggc
     1261 ttggaaccag aggaggtctt tggtgcctgt gccgcagctg ccatgtatcg tcgatcagac
     1321 tttctgaaag ttggtggttt tgacgaagac tatttttctt actttgaaga tgtcgacctg
     1381 agttttcgac ttcgtctagc aggtggacgc tgcctttatg tgccaaacgc tgtggttcac
     1441 catgtgggct ctgctagcac gggaaagcta agcgattttg tcgtatatta cggacatcgc
     1501 aacctagtct ggactttttt taaggatatg cctggcattt tgttttgggt ttacctgccc
     1561 gtacatttat tgatgaatct ttttttcttt ttctctttcg tattcaaagg taaaggctcc
     1621 gctatcctac gatcaaaatg ggatgctttc cgaatgctcc cgcgtatcct gaaaaaaaga
     1681 aaacagatac aaagctcccg aagaatatct tataaagata ttcttgtatt tatgcgcaaa
     1741 agaactttag acccttactg ggcatcgagg caacgtaagc tagcgagcaa tcgttaaagt
     1801 atgacctcct ctctcatcac cctctccatc gtcagtcatg gcgattcaca gaagatttcg
     1861 aatgtactcg ccagcctgct aaaccacgag cagaatactg cctcgcgctt tcaattgatc
     1921 ctcacagaca atctgaagga taacctaccc gatacaaacc ccgctccctg gaagtctttg
     1981 catatgttgc gaaatgacca acctttgggg tttgccgaaa atcataaccg cgctttcaaa
     2041 ctggcgcgcg gtacctactt tgccatcctc aaccccgacc tgatttttga acaaccagtt
     2101 tttgcttcgc tactcaccag ccttcaaact cataatgcag acctgattgc cccaacaatt
     2161 gtggatgaga ccggcacact ccaggattcc tttcgttcgc ttcccactcc cattgagctg
     2221 attcagcgcc gcatgccagg ttatacattc cagcctcctc tgccagacgc agacggcatg
     2281 atccgtcctg attggattgc aggcatgttc tggttgctac gtgctgatac ctatcgtcaa
     2341 ttagggggca tggataaagg ctatcgcctc tattttgaag atgtggattt ttgcacccgt
     2401 gccagacttc aaggcatgaa tctactcgta gattcaaatg taaaggttcg gcatgatgcc
     2461 cgtcgctcaa gtcgcgtgaa actatactac ttggcattac acactcgcag tgccttgcgc
     2521 tttttcacgt ctcctgtata tcgcaagatt cgcaaaattc gctaacttct cttcctgttc
     2581 caagggggta tcatgggctt tcatcaatct ctgataaact actgggtgca tcttacagtc
     2641 ttggaagcga acccacatga gtgacccact cttttttggt ataaccagct taataatgag
     2701 ccttgctctc ggaattccgg ctgcaatttt ggcgcagcgc tttaatctta tggatgtacc
     2761 tggctctgca ccacataagc aacatgctcg cccaacccct ttggcaggcg ggcttttgat
     2821 actacttgtt ttttgtctaa cttttctact taaaccttca tataaccatg aaataattgc
     2881 tgtcttgctc gcgggattga ctattttctt ctttggtctt tgggatg
//
LOCUS       DAOQJE010000450         2920 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_23168_length_2920_cov_6.572426, whole genome shotgun sequence.
ACCESSION   DAOQJE010000450 DAOQJE010000000
VERSION     DAOQJE010000450.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2920)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2920)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2920
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23168_length_2920_cov_6.572426"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2607)
                     /locus_tag="PLT08_11640"
     CDS             complement(<1..2607)
                     /locus_tag="PLT08_11640"
                     /inference="COORDINATES: protein motif:HMM:NF024583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HNE05165.1"
                     /translation="MPKIQNEKFVSSLDEWRADLLNLILGGIFVFWVLAFIGSIRNVI
                     DLYNKSQSLLNNPKLVAISVIVFYGIVTTLVGYVTFNRRLNVNLRAGILLFSLYALGT
                     SGMLLTSFSGDGRVLLFAFVIFSGIFFNLRYSLAAFFVTLLTLVVIGYVQISGLMVVP
                     PEWQVNAANPGAWFSGGLVLIVLSVAGIYSIAYLLQSFEASLKETQEALQREQRLGGV
                     LRTVSEINQIIVRAQDPEKMLHDACELLVAGQGYSFAWVGLLDEDMSTLRHIAHAGGD
                     VDESEFTTRLDLESGLTCAIQAIRTRSFFIVNPSDRNGDPCEKCPRIGKFPNRSAISL
                     PLQRDAKIFGVLVVDHTELAAAFDEQEISLLVELAGDLSYALENLEASKRLDTIARYQ
                     TLLNEVTQTALSAFDLDTLLKDFIEKLEKALDADGYYFAMWDEARGVPSKFIFSQKFH
                     DIFVTPPLLEPDDKFFTRAILEEKRILIIQNVMDTPYINPAFAEQFNVQSAMGIPLIA
                     DQMRIGALVLGYSDQHVFTSEEVKLADQSMNQFALAILKVKLNDEMRAKAKELEQLYT
                     AAQDMTASLMDPPALLKEMARHMAEALDVTSTYITSFDLQVMSMKVVAEYWGKESAPA
                     ELKSDLGRDYPSADYATVLKAMMEGRSLVLHAGQPNLTSEEYQQFVDYHIETMMFVPI
                     YSRGRLLGTVELWESRKRREFSQAEIRLANAMTSHAANIIENSTLFALTRKRESELSA
                     LLSVTRAISSSLQLPDVLREATTTLARMMRVDFCSISDYLPESNLLITRAIYSAEDDV
                     SEQGDIGKEFSLEHYPATLYVLQTGKPLVTRMDDAGADPAEVQQLKNDGMFSSLLIPL
                     RVRGYSLGLAELF"
BASE COUNT          867 a          692 c          579 g          782 t
ORIGIN      
        1 gaataactct gccaaaccta gcgaatatcc acgtacccgt agcggaatca acaacgatga
       61 gaacatccca tcatttttca gctgttgaac ttcggcaggg tcagcacctg catcatccat
      121 acgggtgacc agaggcttcc cagtttgaag cacatacaag gttgccggat agtgttctaa
      181 tgaaaattct tttccaatat ctccctgttc gctaacatca tcttcggctg aataaatagc
      241 acgagtgatg agcaaattac tttcagggag ataatctgaa atggaacaga agtccacgcg
      301 catcatacgt gcaagagtag tggttgcttc acgaagaacg tcagggagtt gcaaactgga
      361 tgaaatcgcg cgagtaaccg ataataaggc gcttaattca ctttcccttt ttcgggtcag
      421 tgcaaacagg gtcgaattct caatgatatt cgcggcatga cttgtcatgg cattagcaag
      481 gcgaatctct gcctgactaa attctcgacg tttacggctc tcccaaagtt cgaccgtgcc
      541 aagtaatcgt ccgcgagaat agatcggaac gaacatcatg gtttcgatat gataatcaac
      601 aaattgttga tattcttctg aggtcaagtt aggttgccca gcatgaagta ccagagacct
      661 gccttccatc attgccttca aaacagtggc atagtcagca ctggggtaat ctctgccaag
      721 gtcagactta agttcagcag gcgcagattc tttgccccag tattctgcca ccactttcat
      781 tgacatcact tgtaaatcaa atgaagtgat gtatgtgctt gtgacgtcca aggcttctgc
      841 catatgacgc gccatttctt tcagcaatgc cggcggatcc atcaagctgg cagtcatatc
      901 ttgagcagcc gtgtaaagct gttctaactc ttttgctttg gcacgcatct cgtcgtttag
      961 tttcacttta agaatggcga gcgcaaactg attcatggac tggtcagcta gtttcacctc
     1021 ttctgaggta aatacatgtt gatcactata gcccaaaact agggcaccaa ttcgcatttg
     1081 gtctgcaatt aatggaatgc ccatggcaga ttgcacatta aactgttctg caaacgcagg
     1141 gttaatataa ggcgtatcca tgacgttttg tatgataaga atgcgctttt cctccaaaat
     1201 agcgcgggta aaaaacttat catctggctc taaaagcggc ggagtcacaa aaatatcgtg
     1261 aaatttttga gaaaaaataa acttggaagg aacacctcgc gcctcatccc acatcgcgaa
     1321 atagtatcca tcagcatcta aagctttctc aagcttttca atgaaatctt tcaacaacgt
     1381 atcaaggtca aatgcagata aagctgtttg tgtaacttca ttcaatagag tttgataccg
     1441 agcaattgta tccagtcttt ttgatgcttc aaggttttca agggcatagg ataaatcacc
     1501 tgccaactcc accagcaagg aaatctcttg ttcatcaaag gctgccgcta gttctgtatg
     1561 gtcaacaacc agcacaccaa atattttcgc atctcgctgt agaggtaaag agatcgcaga
     1621 cctgttcggg aatttcccta tgcgggggca tttttcacat gggtctccgt tacgatctga
     1681 tggattaaca ataaaaaatg agcgggttcg aatcgcctga atggcacagg tcagcccgct
     1741 ctcaaggtcg aggcgagtag taaattcgct ttcatccaca tcacctcctg catgagcaat
     1801 atgccgaagt gtgctcatat cctcatccaa caaacccacc catgcaaagg agtacccttg
     1861 cccagcaaca agaagctcac aggcgtcgtg aagcatcttt tctggatctt gtgctcgaac
     1921 aataatctga ttaatttcac tgaccgtacg cagaaccccg ccaaggcgct gctcacgttg
     1981 taaagcttct tgggtttctt tcaagctggc ttcaaaagat tgtaagaggt aagctatcga
     2041 ataaatccca gcgacactta atacaatcaa caccaaccca ccactgaacc atgccccagg
     2101 gttggcggca ttcacctgcc attctggcgg aaccaccatc agcccagaaa tttgcacata
     2161 cccaatgacc actaatgtta gcagagttac gaaaaaggca gccaaactat agcgtagatt
     2221 aaagaatatc cctgaaaaga ttacaaacgc aaataaaaga actctgccat ccccactgaa
     2281 cgaagtgagc aacattccgc ttgtgcccaa agcatataaa ctaaaaagca gtattcctgc
     2341 acgcaggttc acattcaatc tacgattgaa tgtgacataa ccaaccaatg tggtcactat
     2401 gccgtaaaac acaataaccg aaatcgcaac cagttttggg ttattcagta ggctctgact
     2461 tttattgtat agatcaatga cgtttcgaat gcttccaata aatgccaaga cccaaaaaac
     2521 gaaaattcca cccaagatta agttcaaaag gtcagcccgc cattcatcta acgatgaaac
     2581 aaacttctca ttttgaatct tgggcatagc aaaaaatgcg tctttgagtc tttgatacat
     2641 aatgatttag gctattttta aattatagtt gcattttttg cctgaaacct ttcagagtta
     2701 gtacggtttt gcccaaaaac gccccttctg caagcctttt tcattaaata ttatgatatt
     2761 tatcacatgg acaggtggaa atacagacag gtatactacg ccaatctttt tagagaattt
     2821 ttgtctgcaa tccaactcga ggagaaatca tgtccgctag ggattctgtt ccttcttcta
     2881 aattcgccat gttctggtca ttgaccaaac cctcgcaatc
//
LOCUS       DAOQJE010000451         2920 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_23169_length_2920_cov_6.523560, whole genome shotgun sequence.
ACCESSION   DAOQJE010000451 DAOQJE010000000
VERSION     DAOQJE010000451.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2920)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2920)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2920
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23169_length_2920_cov_6.523560"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..337
                     /locus_tag="PLT08_11645"
     CDS             <1..337
                     /locus_tag="PLT08_11645"
                     /inference="COORDINATES: protein motif:HMM:NF014471.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015109 - chromate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015703 - chromate transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="chromate transporter"
                     /protein_id="HNE05166.1"
                     /translation="FAGYAAAGVPGVFTALLGAMLPPTVMMFIVAFILQQVQHEDWLT
                     GFVKGMSPAVAALIVMVAWELFRVGQTKAIGKRTLIIAALSAIALWFDVPSPFVLLGA
                     GILGVILFR"
     gene            349..1194
                     /locus_tag="PLT08_11650"
     CDS             349..1194
                     /locus_tag="PLT08_11650"
                     /inference="COORDINATES: protein motif:HMM:NF012791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HNE05167.1"
                     /translation="MSSDILIVTNGTKESFPAVEQGAWLAKTLERKITLLGITEKLNP
                     AAIDDTHPLEEVFEKAVDVFNAQGAAYRLEVRNGSAEKIVPQEAKVQDSIVVLAPLGR
                     PQLQRLMAGRSIRNFIEAIEQPLLYVPSVRKTVQKILVSVGGLGYDVNAEHIAMQIAM
                     KSGAEIVFLHVVPPIDLNYPTAEAISKNWQYLATTDTPVGRSLRQSLENARKAGINAS
                     VKVRHGNVIEEILAESVEGKYDLLCMGSSYSVHSLRQMYSANVTSEIMERVQCPVMTA
                     RFRTE"
     gene            1197..1748
                     /locus_tag="PLT08_11655"
     CDS             1197..1748
                     /locus_tag="PLT08_11655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HNE05168.1"
                     /translation="MNDTTGFVLKALRFAAEKHNDQRRRDSKSSPYINHPIQVAETLW
                     RVGGVQDPALLVAAILHDTIEDTDATPDEIRREFGEEVLELVLEVTDDKSLPKEVRKQ
                     LQIVNAPKKSSRGKMLKLADKICNVYDLIHFPPSNWSLSRKQEYLLWTEKVVQGVRGS
                     NKDLEARYDEVLEEGKRVLDISD"
     gene            1836..2672
                     /locus_tag="PLT08_11660"
     CDS             1836..2672
                     /locus_tag="PLT08_11660"
                     /inference="COORDINATES: protein motif:HMM:TIGR00762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005504 - fatty acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HNE05169.1"
                     /translation="MTIIVADTTCGLPRALLAQRGISLIPQVVMFGEESFHDDKDLDT
                     ALFLQKLKAAKALPKTAAPEPSLYYPILEEARSKNESVVVVAPTGKASGTVRSAQTAA
                     QEFPDVDVRVVDTQTISCNLGSLVLLADDMAKVGKSASQIVAKLEDMIPRGRIYFLVD
                     TLEYLAKGGRIGGAKRLVAELLEIKPILQIKDGQVESFEQQRTKKRALARLVEITKEQ
                     CSGGAESHLCVLQVEAEKEAQALVEELKSTIPVTEIPIYELPPAIVVHAGPRAMGIGF
                     FV"
     gene            2706..>2920
                     /locus_tag="PLT08_11665"
     CDS             2706..>2920
                     /locus_tag="PLT08_11665"
                     /inference="COORDINATES: protein motif:HMM:NF013122.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease domain-containing protein"
                     /protein_id="HNE05170.1"
                     /translation="MFYNARTMLELSNTRFAFLDVETTGLSPWFGDRICQIAVIVTEG
                     KRIKRTLDLLVDPERELSPSAIHVNGL"
BASE COUNT          768 a          595 c          785 g          772 t
ORIGIN      
        1 gtttgcaggc tacgctgctg ctggggttcc gggtgttttt acggcattat tgggtgccat
       61 gcttccaccg accgtcatga tgttcattgt ggcatttatc ttgcaacaag tgcagcatga
      121 agattggctg acggggtttg tcaaagggat gagccctgcg gtggctgcct tgatcgtgat
      181 ggtggcgtgg gaattgttcc gcgtggggca gaccaaggcg attggcaaac gcaccctcat
      241 catcgccgct ttaagtgcga ttgcattgtg gtttgatgtg ccatctccct ttgtgttatt
      301 gggtgcggga attctagggg ttatcttatt ccgttgattg gaattgttat gtcgtccgat
      361 attttgatcg ttacaaatgg aaccaaagaa agtttccctg ctgtggaaca gggtgcctgg
      421 cttgcaaaaa ctttggaaag gaagatcacg ctgttgggaa tcaccgaaaa gttgaacccg
      481 gcagcgattg atgatacaca tccgctcgaa gaggtttttg aaaaagccgt ggatgtattc
      541 aatgctcagg gggcggcata caggcttgag gtccgcaatg gaagcgcgga gaagatcgtg
      601 ccacaagagg cgaaggtgca agactcgatt gtggtcttag cgccgttggg acgtccgcaa
      661 cttcaacgtt tgatggcggg gcgttcgatc cggaatttta tcgaagcgat tgaacagcct
      721 ttgctgtatg tgccaagtgt gcgaaagacc gttcagaaga ttttagtgag tgtgggcgga
      781 ttgggctatg atgtgaatgc cgaacacatt gccatgcaaa ttgcgatgaa aagcggcgca
      841 gagattgtct ttttgcatgt ggtgccgcct attgatttga attatccgac cgccgaagcg
      901 atcagtaaaa attggcaata tctggcaaca acagatacac cggttggacg aagtttgcgc
      961 caatcgttgg aaaatgcgcg caaagcgggc attaatgcct ctgtgaaagt gcgacatgga
     1021 aatgtgatcg aagagattct tgctgagtct gtggaaggca agtatgacct tttgtgcatg
     1081 gggtcttcgt atagtgtgca ttcattgcgg cagatgtatt ctgccaatgt aacttcagag
     1141 atcatggaac gcgttcaatg tcccgtgatg acggcaaggt ttagaacgga gtaaatatga
     1201 acgatacaac tgggtttgtg ctcaaagcac ttcgctttgc agcggaaaaa cataatgacc
     1261 aacgtcgaag agactcaaag tcttcgccgt atattaatca tcccattcag gtggctgaga
     1321 ctttgtggcg agtgggcggc gtgcaagatc ctgctctttt agttgctgcg attctgcacg
     1381 ataccattga agatactgat gccactccag atgaaattcg gcgcgagttt ggcgaagaag
     1441 ttttagagtt ggtgcttgaa gtaaccgatg acaaaagcct acctaaagaa gttcgtaagc
     1501 aattgcaaat cgtgaatgcg cctaagaaat cttctagggg aaagatgctc aagttggcag
     1561 ataaaatatg caacgtctat gacttgatac attttccacc ttcgaattgg tcactaagcc
     1621 gaaaacaaga atatttgttg tggacagaaa aagtagtaca aggcgtacgc ggctctaata
     1681 aagatttaga agctcgttac gacgaggtgt tggaagaagg aaaacgtgtt ctagacattt
     1741 cggattgaga cccacaaagt ttgtaatacg aaacttagtt cttttgacag ttcaaccagc
     1801 tttcaaaggg gaatacaatc aaataggagg ctgccatgac cataattgtt gccgatacca
     1861 catgtggact tccccgcgct ttacttgctc aacgcgggat ttcattgatt ccgcaagttg
     1921 tgatgtttgg ggaagagtct tttcatgatg ataaagattt ggatacggct ctgtttctac
     1981 aaaagctaaa agcggcgaaa gcgcttccga aaacagctgc tcctgaacct tcgctgtatt
     2041 atccaatttt ggaagaagcc agaagcaaaa atgaaagtgt ggttgttgta gctccaactg
     2101 gtaaagcgag cggaactgtc cgctcggcac agacagcggc acaggaattt ccagatgtcg
     2161 atgtgcgtgt cgtggataca cagacgattt cctgtaatct cggttcgctt gtactgttgg
     2221 cagatgatat ggcgaaggtg ggcaaatcag cgagtcagat cgttgctaaa ctagaagata
     2281 tgattccacg tggaagaatc tattttctgg tcgatacact agaatatctt gccaagggcg
     2341 ggcgcattgg cggcgccaaa cgtctcgtag cagagttgct tgaaatcaaa cccattctac
     2401 agatcaaaga tgggcaagtt gaatcttttg aacaacagcg aaccaaaaaa cgtgcattgg
     2461 ctcgtttggt agagattacc aaagaacaat gttccggtgg cgctgaaagc catttgtgtg
     2521 tgctgcaagt tgaggctgag aaagaagcgc aggcattggt tgaagaattg aagtccacaa
     2581 tacctgtcac agagattccc atctacgaat taccaccagc tatcgtagtg catgccggtc
     2641 cgcgcgcgat ggggattggt ttttttgtgt aatagaaaag tcgtcagtaa agtactgacg
     2701 acttcttgtt ttataatgcc cgcactatgc ttgaactttc caatacccgc tttgcatttt
     2761 tggatgtcga aaccactggg ctttcacctt ggttcggaga tcgcatctgc caaattgctg
     2821 tgatcgtgac cgagggcaaa cgcatcaaac ggacccttga cctgctcgtt gaccccgagc
     2881 gtgagctttc gccgtcagcg attcacgtca atggcttgga
//
LOCUS       DAOQJE010000452         2920 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_23173_length_2920_cov_4.120768, whole genome shotgun sequence.
ACCESSION   DAOQJE010000452 DAOQJE010000000
VERSION     DAOQJE010000452.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2920)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2920)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2920
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23173_length_2920_cov_4.120768"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..901)
                     /locus_tag="PLT08_11670"
     CDS             complement(<1..901)
                     /locus_tag="PLT08_11670"
                     /inference="COORDINATES: protein motif:HMM:NF013696.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HNE05171.1"
                     /translation="MTQKNNMHERPVEILQRLIQFNTTNPPGHEAACVGYIRDLLNEA
                     GIESTLVAKDPDRPNLIARLPGEGKAPPLLLYGHVDVVTTESQRWQQAPFEGKLIDGY
                     VWGRGALDMKGGVAMMVAAFMRANAEGLRPPGDIVLAIVSDEEAGGDYGAKFLVEEHP
                     ELFKDIKHAIGEFGGFTLTLGGKRFYPIMISEKQVCWMKATVRGQGGHGSMPEKGGAT
                     AKLAKFLKALDEKDLPVHVTPATKLMVDAMASALGGLQGFILAQLTNPALTDLVLKTL
                     GSRVRSFYPLFRNTASPTIIHSSD"
     gene            1031..1471
                     /locus_tag="PLT08_11675"
     CDS             1031..1471
                     /locus_tag="PLT08_11675"
                     /inference="COORDINATES: protein motif:HMM:NF024854.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HNE05172.1"
                     /translation="MSTATQLREQALDGFWETIPPLWNFVRSYIRATATSKFDITVEQ
                     FHVLRFVRKGMSISELAIAKNISRPAISQAVDVLVKKGLLTRVQSTKDRRYVTLALTE
                     KGNALLDTVFKETRTWMKGRMSKLTNEELESIAKAMQAFKKILP"
     gene            1493..2764
                     /locus_tag="PLT08_11680"
     CDS             1493..2764
                     /locus_tag="PLT08_11680"
                     /inference="COORDINATES: protein motif:HMM:NF017760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNE05173.1"
                     /translation="MNKDIKEPIEPILSKNEVRRSGETTFVAMRHRNYQLYFGGQLIS
                     NIGTWMQVIAQAWVVYQLGHSELTLGLVAFASAIPNLIVSPWGGVIVDRISRRKVLIM
                     TQSGAMVLSFILAWLTFTNIVQEWHVILLAALTGVITAFDAPARQAITPELVGKKDVA
                     NAIAMNSMMFNSARVVGPAIGGMMLATVGAAWCFTVNGISFLAVLIGLWLMKLPPHRK
                     SAQDVSPWKQLVGGLQYAASNIEISTLILLSLVFSIFGVSYTTVLPAFVEKNLQLGAT
                     AFGWVNAVTGLGAVTGAFLLVHRISNNRRGLFLIVTNIAFPVTLIAFAVTAFYPLSLV
                     LAYLLGLGFMVQFTTINTLLQARVEDDFRGRVMGLYTLSFFGFSPFGNLLMGALGEKF
                     GLTFVMILFGILSLVLSRLVLARNPGIKALP"
BASE COUNT          711 a          753 c          692 g          764 t
ORIGIN      
        1 tatcactgct atgaataatc gttgggctgg cagtattcct aaacaatgga tagaacgaac
       61 gcacccggga accaagggtt ttgagcacaa gatccgtcag cgcagggttg gttagctgag
      121 cgaggataaa accttgaagt ccgcccagag ccgaagccat tgcatccacc atcaattttg
      181 ttgctggagt cacatgcaca ggcaagtctt tttcatccaa cgcttttaag aacttcgcca
      241 atttcgctgt cgcgccgcct ttctctggca tcgaaccgtg accgccctgt ccacgcacag
      301 tggctttcat ccaacagact tgcttttcag agatcatgat cgggtaaaag cgcttgccgc
      361 caagagttaa agtaaaaccg ccaaattcac cgatggcgtg ctttatatct ttaaataact
      421 caggatgttc ctccacaaga aactttgcgc cgtaatcgcc acccgcctct tcatcgctga
      481 cgattgccag cacaatatcc ccaggcgggc gcaagccttc agcattggcg cgcatgaaag
      541 ccgccaccat cattgccacg ccgcccttca tatctaaagc gccacgcccc catacatatc
      601 catcgatgag cttgccctca aatggagctt gctgccagcg ttgactctcg gtcgtcacca
      661 catccacatg cccatacaac aacaaaggcg gagccttacc ctcaccgggc aggcgtgcga
      721 tcaaattagg tcggtctggg tctttggcta ccagcgtgga ttcaatgccc gcctcattca
      781 acaaatcacg gatgtagcct acacaagccg cctcgtgacc gggcgggttg gtcgtgttga
      841 actggatcag ccgttgaaga atttcaacgg gtcgttcatg catattattt ttttgcgtca
      901 ttgaaagctc caatctatag atggcttcca aattatatgc ctaatcccct tcttgttgtt
      961 tttcgtaaac aggcataaaa tagtttcaat acttaactat taataaaatt acacaatttg
     1021 aaaggtttca atgagcactg ccacccaact ccgtgaacaa gccttggacg gcttctggga
     1081 aaccattccc cctctttgga attttgtccg ttcgtacatt cgagccaccg ccaccagtaa
     1141 atttgatatt acggttgaac agtttcatgt actacgcttt gtgcgcaagg gcatgtcaat
     1201 tagtgagctg gcaattgcga aaaacatcag tcgtcccgcc attagtcagg ctgtagatgt
     1261 tttggtcaaa aaaggattat tgacccgcgt gcaaagcaca aaagatcgtc gatatgtgac
     1321 ccttgcgctg accgaaaaag gcaacgcctt gttagataca gttttcaaag aaacgcgcac
     1381 atggatgaaa ggtcgcatga gcaagttaac caacgaagaa ttggagtcga ttgccaaggc
     1441 gatgcaagcc ttcaaaaaaa tattgcccta actttaggag tttgacataa gaatgaataa
     1501 agatataaaa gaaccgatag agcctattct ttcaaagaat gaagtgcgta gaagcgggga
     1561 aacaaccttt gttgccatgc ggcatcgtaa ttatcagttg tactttggcg gacagttaat
     1621 ctccaacatc ggcacgtgga tgcaggtcat tgcacaggct tgggtggtgt atcagctcgg
     1681 tcactccgag ttgaccttgg ggttggtcgc ctttgcttct gccattccaa acttaatcgt
     1741 ctctccatgg ggcggtgtga tcgtagaccg catatcgcgc cgtaaagtat tgataatgac
     1801 ccagtctggt gcaatggtgt tgtctttcat cctcgcctgg ttgacattta ccaacatcgt
     1861 gcaggaatgg catgtgattc tgctggcagc cttgacgggt gtgattaccg cctttgatgc
     1921 cccggcacgt caagccatca caccggaact cgttggaaag aaagacgtcg ccaacgccat
     1981 tgcaatgaac tcgatgatgt tcaacagtgc ccgtgtggtg ggacccgcca tcggtggcat
     2041 gatgctggcc acggtcggtg cggcgtggtg ctttacggtg aatggcattt ccttcctcgc
     2101 ggtgttgatt ggtctgtggc tgatgaagct acctccccat cgcaagtcgg cgcaggatgt
     2161 ttctccatgg aaacaattgg taggtggtct gcaatatgcg gcaagtaaca tcgaaatctc
     2221 gacattgatt ctgctctctc tcgtattcag catcttcggc gtttcgtata caaccgtctt
     2281 gccagccttc gtagaaaaga acttacagct tggtgccacc gcctttggct gggtcaatgc
     2341 tgtgaccggg ctcggagccg taacaggcgc ttttctattg gtacatcgca tcagcaacaa
     2401 ccggcgcgga ctctttctca tcgtcaccaa tatcgccttc ccagtaacat taatcgcgtt
     2461 cgcagtgacc gctttttatc cgctttcact ggtgctcgct tatttattag ggcttgggtt
     2521 catggtgcaa ttcacaacca tcaacacgct gttgcaagca cgggtggaag atgacttccg
     2581 cgggcgcgtg atggggcttt acaccctaag ctttttcggc ttttcaccct ttggaaatct
     2641 cttgatgggt gcactgggcg aaaaattcgg tttgaccttc gtaatgatcc tttttgggat
     2701 tctttcgctg gtactctcgc gtctggtgct tgcccgaaac ccagggatta aggctttgcc
     2761 gtagctctta aaaagaaaag ccgtcgttag aaacgacggc ttttcttttt aaatacaaac
     2821 tacttcttat cgccaccgag caagccaccg agcagacctg cggcatcgtc gagaccgatg
     2881 ccatcgctta ggtcaacgtt tccggcgaga atggcatcaa
//
LOCUS       DAOQJE010000453         2918 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_23203_length_2918_cov_5.942019, whole genome shotgun sequence.
ACCESSION   DAOQJE010000453 DAOQJE010000000
VERSION     DAOQJE010000453.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2918)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2918)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2918
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23203_length_2918_cov_5.942019"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1530)
                     /locus_tag="PLT08_11685"
     CDS             complement(<1..1530)
                     /locus_tag="PLT08_11685"
                     /inference="COORDINATES: protein motif:HMM:NF040095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HNE05174.1"
                     /translation="MLKNLQDIKPRILLVDNDPDSCTSYKDLLQQWGYNPILATGLGT
                     ALKDDARKKAEECRCSLALIDLRLIDDDDEMDMSGAEFAKSLQSLLHPIILTGIEDPT
                     TLRKLLQDYKDITYLGKQDRRDEFRQRLDAEASKVAASKRQLEFVQTELLDEFLKSDL
                     VKSTQEHANQIVDVLARLFPEAKVLRFEKVSGWGETEVSSAVRPNSIVIKVYEDSLEP
                     CLVKLARAKKVDHEVRSYFEYIERKLTGSFNAKLLQKAIVWDIGGAAYTFISGNGTRT
                     FSAYYKDQPIQSIKEVLTSFFRDMWKKYYNQATPEKKSLFNLYLEVWGQDWYEKCANE
                     LTSAVLDEELSAISKNIGLPNPIEWLNNKIQKNEMVFDTKIAVTHGDLHGDNLMVDDR
                     KNVWVIDFERCGKGHALQDFIELEADVINRLLGHPVNFPAYLKMCKTVFQTTEINLEE
                     ADLVSEDVEIEKALKTISIIRDLATQCTGMNDAREYLYGLLYNMTFRAQLLHKIDKSK
                     SG"
     gene            complement(1542..>2918)
                     /locus_tag="PLT08_11690"
     CDS             complement(1542..>2918)
                     /locus_tag="PLT08_11690"
                     /inference="COORDINATES: protein motif:HMM:NF024583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNE05175.1"
                     /translation="MLSIHSDADTIIFTGYDTPEDGLRAYEAGASRYLPKPFEPEELE
                     FVLKELMRSRKVRLAEARQRQQFRVATKIAEAVGASLNLEDTMDAVLGTLYSVFDKTR
                     LCVLLYDERQNALRFAPSTMKYYEIKNTQYSYQDTFPLNAGSLACQAAQLTIRHKSME
                     LVNVGDVTQDTEYLNLNPDTKSECCVGLLNMHGELLGVLALEREWLNGFDERDLDLIH
                     ITARHISIAIERAHQSEELKSKSAIAAQTSWAAALAHELNNEVGKIANWAQVIQQVIG
                     DDLPEIRNYAKNIEESAYYLSRSNPWSARSIKSFEIDQHIKDVLEKLAPKKSLSIEFR
                     LNAQGAKVKVSPAQFQFILKQLAGNAARAMGNNEDKKVLVETQLANLETVEILFQDFG
                     PGISEENRMAIFNWQFTTKETGGFGLLFVKQMIEDMNGNIMLLPNQPGKGAAFLIHLP
                     LEKKKISE"
BASE COUNT          726 a          613 c          653 g          926 t
ORIGIN      
        1 gccgcttttg cttttatcta ttttatgcaa aagctgagca cggaaggtca tgttatagag
       61 cagcccgtat aagtattcac gtgcatcatt catgcctgtg cattgggttg ctagatcgcg
      121 aatgatagag atcgttttga gtgctttttc aatttctaca tcttccgaga ctaaatctgc
      181 ttcttctaaa ttgatttcag ttgtctggaa aacggtctta cacattttta gataggcggg
      241 gaaattgacc ggatgcccca aaagacgatt tatcacatcg gcttccagtt caatgaaatc
      301 ttgaagggcg tgccctttgc cgcaacgctc aaaatcgatg acccagacat ttttgcgatc
      361 atctaccata aggttatcac catgtaggtc gccatgggta acagctatct tggtgtcgaa
      421 aaccatttca tttttttgaa tcttattatt taaccattct attgggttag gaagcccaat
      481 attttttgaa atagcagata gttcttcatc aagaacagca gaagtcaatt cattggcaca
      541 tttttcgtac caatcttgcc cccatacttc cagataaagg ttgaacaagg actttttttc
      601 tggagttgct tggttgtaat attttttcca catatccctg aaaaaagaag ttaaaacttc
      661 tttgatgctc tgaatgggtt ggtctttgta ataggcagag aaagttcttg tgccatttcc
      721 gctaataaaa gtatatgcgg ctcccccaat atcccaaacg atggctttct gcaggagttt
      781 tgcattgaaa ctacctgtga gctttctttc aatatactca aaatagcttc ttacttcatg
      841 gtcaactttt ttcgcacgtg ccaatttgac aaggcatggt tcaagtgagt cttcatagac
      901 tttgattacg atagagtttg gacggacggc tgaagagact tcggtttctc cccaaccact
      961 aactttttcg aagcggagta cttttgcttc gggaaagagg cgggcaagga catctacaat
     1021 ttgattcgca tgttcttgtg tactcttgac gaggtcagat ttgaggaatt catccaataa
     1081 ctcggtttgg acaaactcaa gttggcgttt agaggcggca acttttgagg cttctgcatc
     1141 cagccgttga cggaattcgt cgcgccggtc ttgtttgcca agatatgtaa tatctttata
     1201 gtcttgtaaa agtttgcgca aggttgtggg gtcttcaatg cctgtgagga taattgggtg
     1261 aagcaaagat tgcaaggact tggcaaattc agcgccactc atatccattt cgtcatcgtc
     1321 atctatcaaa cgcaggtcaa ttagagccaa actgcagcgg cattcttcag cttttttcct
     1381 tgcatcatcc tttaatgctg ttccgagccc tgttgccaga atcgggttat agccccattg
     1441 ttggagaaga tctttatatg aagtacagct atctggatcg ttatcaacca gaaggatgcg
     1501 tggcttaatg tcttgcaggt tttttaacat agatgtttag gttattcaga aattttcttt
     1561 ttttcaagtg gcaaatggat taagaacgcg gctcccttac ccggctgatt gggtaaaagc
     1621 ataatattgc cattcatatc ttcaatcatt tgttttacga aaagcaaacc gaaaccgcca
     1681 gtttcctttg ttgtaaactg ccagttgaag attgccatac ggttctcttc gctaatgccg
     1741 ggaccaaaat cttgaaacag gatttccact gtttctaggt ttgcgagttg agtttcaacc
     1801 agaacctttt tatcttcatt gttgcccata gctcgagctg cgtttcctgc tagttgtttg
     1861 agaatgaact gaaactgagc aggggagacc tttacttttg caccttgagc attcaaccga
     1921 aattcgatag aaagactttt ctttggtgcc agcttttcaa ggacatcttt aatatgttgg
     1981 tcaatttcga agctcttaat cgagcgagca gaccaaggat tcgaccgtga gagatagtaa
     2041 gcgctttctt cgatgttttt ggcgtaattt ctaatttcag gaaggtcgtc gccgatcact
     2101 tgttgaataa cctgtgccca gtttgcgatc ttgcctactt cattgtttag ctcgtgggca
     2161 agggcagctg cccacgaggt ttgtgctgcg atcgcggact ttgatttcag ttcctcactt
     2221 tgatgagcac gttcaatggc gatgctaatg tgacgtgcgg taatatggat aaggtcgagg
     2281 tctctttcat cgaacccatt gagccattca cgctctaacg ccagaacgcc taaaagttct
     2341 ccatgcatat tgagtaaacc gacacaacat tctgattttg tgtcaggatt taggttgaga
     2401 tattctgtat cttgagtcac atccccaaca ttgaccagtt ccatgctctt gtgccgaatg
     2461 gtcaattgag cggcttggca ggcaaggctg ccggcgttta gcggaaatgt atcttgatag
     2521 gagtattgag tatttttgat ctcataatac ttcatggtgc taggcgcgaa ccgtaaggca
     2581 ttttggcgct catcatagag taggacacac aggcgtgtct tatcgaatac tgaatacaat
     2641 gtgccgagta cagcatccat ggtgtcttcg aggttcaagc tggcgcctac agcttctgca
     2701 attttggtgg cgactctaaa ttgttggcgt tgacgcgcct ctgctagacg cacttttctt
     2761 gagcgcatta gttctttgag gacgaattcc agctcctctg gttcaaaagg tttcggaagg
     2821 taccgtgagg ctcctgcttc ataggctcgt aagccgtctt ctggggtgtc gtaccctgtg
     2881 aaaataattg tgtctgcatc cgaatggatg gataacat
//
LOCUS       DAOQJE010000454         2917 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_23217_length_2917_cov_6.745982, whole genome shotgun sequence.
ACCESSION   DAOQJE010000454 DAOQJE010000000
VERSION     DAOQJE010000454.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2917)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2917)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2917
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23217_length_2917_cov_6.745982"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..909
                     /locus_tag="PLT08_11695"
     CDS             <1..909
                     /locus_tag="PLT08_11695"
                     /inference="COORDINATES: protein motif:HMM:NF019607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HNE05176.1"
                     /translation="IFKDGHLSPQPFLNIDDRVNDDSNEMGLLGLAFHPNFEQNGYFY
                     VNYTGDGGNTRISRFTANGDVADPNSEQVLMVIDQPFPNHNGGAVAFGPDGYLYLGLG
                     DGGAAGDPFKNGQNTETLLGKLLRIDVNNGDPYAIPADNPFGNEVWAYGLRNPWRISF
                     DRATGDLWIGDVGQNQYEEIDYLPAGSPGGANFGWSVMEGLHGYDGEAQPGLLLPVVE
                     YSHEIGCSVTGGYVYRGSMPEWNGVYLYGDYCSGSVSGLILVDGQWQSRVLFEAVATI
                     TSFGQDEAGELYFTSDNGSIYILAKK"
     gene            1020..1241
                     /locus_tag="PLT08_11700"
     CDS             1020..1241
                     /locus_tag="PLT08_11700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE05177.1"
                     /translation="MAKKVWQVEKTKYCEHLQQAIAIEVEVVYPAEQLPEQTPRVMAR
                     RCSKAIECNCVEHPSCSLSGTNPDLDPVE"
     gene            complement(1339..2127)
                     /locus_tag="PLT08_11705"
     CDS             complement(1339..2127)
                     /locus_tag="PLT08_11705"
                     /inference="COORDINATES: protein motif:HMM:TIGR01581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNE05178.1"
                     /translation="MKKIPSRSKWLFILPSIFMLALFLLPLSAILIRSVGDDFFTHAF
                     SEQAFKALRLSLATSTITTLVAIMFGTPLAYMLARWKLRFKTWLELLIDLPIVLPPSV
                     AGLALLIAFGRRGIFGSVLEAFGVTLPFTTTAVVLAQLFVAAPLYIRSARIGFAEVDD
                     QLEEAANVEGANQWQMFAAIMLPLAGRALISGAILTWTRALGEFGATILFAGNLEGVT
                     QTMPMAIYLGFERNVGVALSLSVVLIFVSTLLLLLTRKLEKEKD"
     gene            complement(2176..>2917)
                     /gene="modA"
                     /locus_tag="PLT08_11710"
     CDS             complement(2176..>2917)
                     /gene="modA"
                     /locus_tag="PLT08_11710"
                     /inference="COORDINATES: protein motif:HMM:NF024921.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009897 - external side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015412 - ABC-type molybdate transporter
                     activity [Evidence IEA];
                     GO_function: GO:0030973 - molybdate ion binding [Evidence
                     IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="molybdate ABC transporter substrate-binding
                     protein"
                     /protein_id="HNE05179.1"
                     /translation="QTTPIPVSTTEETTLTVFAAASLTDAFTEIGENFETANPGVTVT
                     FNFAGSQALRTQIEEGAPADIFASASGKEMDAAIEGKFIAEDSNQVFLTNKLVVILPA
                     DNPAGLETLEDLANSGVKIVLAAEEVPVGNYARQALDLMNGSFGADFKDKVLANVVSN
                     EDNVKQVVSKVQLGEADAGIVYTSDAVAAPELKSIEIPAELNVIAKYPIAPLVKSENA
                     DLAQAFMDYVLSDEGQAILAKWGFGPKP"
BASE COUNT          711 a          699 c          804 g          703 t
ORIGIN      
        1 atcttcaaag atgggcacct ttctcctcaa ccttttctca acatcgatga ccgtgtcaat
       61 gatgatagta atgaaatggg gctacttggc ttggcgtttc atccgaactt tgaacagaac
      121 ggctattttt acgtcaacta cacaggcgat ggcggaaaca cccgcatctc gcgtttcacc
      181 gccaatggcg acgttgccga ccccaacagc gaacaggtgt tgatggtcat cgaccaacct
      241 ttcccgaatc acaatggcgg cgctgtagcc ttcggacccg acggttatct ctatctcggg
      301 ctgggcgatg gcggtgctgc aggtgacccg ttcaagaatg ggcagaacac cgaaacccta
      361 ctcggaaaac tcctgcgcat tgacgtgaac aatggtgacc catatgctat ccctgcggat
      421 aatccattcg gcaatgaggt ctgggcatat gggcttcgta acccgtggcg catttcattt
      481 gaccgtgcta cgggtgatct gtggatcggc gatgtggggc agaaccaata tgaagagatc
      541 gactatttgc cagccggctc gccaggcggt gccaactttg gctggagtgt aatggaaggg
      601 ctgcatgggt acgacgggga agcccagcca gggctactgt taccggtagt cgaatacagc
      661 catgaaattg gctgctctgt gacaggcgga tatgtctatc gcggctccat gccagaatgg
      721 aacggcgtgt acttgtacgg cgactattgt tctggctctg tttcagggtt gattctggtc
      781 gatgggcagt ggcagtctcg ggtcctgttt gaggcggtgg caaccatcac ctcttttggg
      841 caagacgagg ctggcgagtt gtacttcacc agcgataacg gcagtattta catccttgca
      901 aagaaataaa gacttatcac atccataata ggtgctttgt catccgagtt tatgacgcct
      961 gtcactggtc tgacaggcgt tttggttcta aactagggac atctgagtca ggagcagtta
     1021 tggcaaaaaa ggtttggcaa gttgaaaaaa ccaaatattg cgagcacttg cagcaagcaa
     1081 tcgccatcga agtcgaggtt gtatacccgg ctgagcaact gcccgagcaa actccccgtg
     1141 ttatggcaag gcgctgttca aaagcaatcg aatgcaattg cgtagaacac ccctcttgct
     1201 ctctaagcgg gacaaatcct gaccttgacc ctgttgaata gagcagcgca caacgctgtt
     1261 catccaaact ctctctcctc cctctgagtt ggtcttaagg ctacgccgcg caacgcgcgg
     1321 cgtaagccgt taaatgggtt agtctttttc tttttccagt tttcttgtga gtaatagcaa
     1381 tagggtggaa acaaaaatga gcaccactga aagcgataat gcaaccccta catttcgttc
     1441 aaagccgagg taaattgcca tcggcatggt ttgcgtgacg ccttctaaat tcccagcaaa
     1501 taaaatggtt gccccaaatt cgccaagcgc gcgcgtccaa gtgaggatgg cgccgctgat
     1561 gagggcacgc cctgccagcg gcagcatgat cgcggcaaac atttgccatt gatttgcgcc
     1621 ttccacgttg gcggcttctt cgagttggtc atccacttcg gcgaagccaa tccgtgccga
     1681 gcggatataa agcggggcgg cgacgaataa ttgcgccaag acgactgccg ttgttgtgaa
     1741 aggtaaggtg acgccaaacg cctctaacac ggacccgaag attccgcgcc gaccgaaggc
     1801 gatcagcagg gcgagccctg ccacggaggg tggcaggacg atgggaaggt cgattagcaa
     1861 ttcaagccag gttttgaagc gtaacttcca acgagccaac atgtaggcta gtggcgtgcc
     1921 aaacataatc gctacaagag tagtaatagt gctagtggcg agacttaaac gcagggcttt
     1981 gaaagcttgt tctgaaaagg cgtgcgtgaa gaaatcatct cccaccgagc ggatcaaaat
     2041 ggcggaaagc ggcaatagaa ataatgccag catgaaaatg ctgggaagga taaagagcca
     2101 ttttgaacga gaggggattt ttttcataga caaaagcgcg ggaggatttt cctctcgcgc
     2161 tagatagtac cttgtttatg gtttgggacc aaacccccat tttgccagaa tggcttgacc
     2221 ttcatcggaa aggacataat ccatgaaggc ttgggcgagg tctgcgtttt cagatttcac
     2281 cagtggagca atgggatatt tggcgataac gttcagctca gcgggaatct caatggattt
     2341 caactctggt gccgcaacgg catcggaagt gtatacgata ccggcatcgg cttcgcctaa
     2401 ttgaactttg gataccactt gcttgacgtt atcttcgttg gagaccacgt tggcgagcac
     2461 tttatctttg aaatcagcac cgaaggaacc gttcattaga tccaacgctt ggcgagcgta
     2521 attgccgacg ggtacttctt cggctgccaa cacgatcttt actccagagt tagcgaggtc
     2581 ttcgagtgtt tccaacccgg cggggttatc ggctgggagg atgacgacca gtttgtttgt
     2641 gaggaatacc tggttcgagt cttccgcaat gaatttgcct tcgatagcgg cgtccatttc
     2701 ttttccactg gcggaagcga agatatctgc tggggcgcct tcttcgattt gggtgcgtaa
     2761 ggcttgcgaa ccggcgaagt tgaaggtgac ggtcacaccg gggttggcag tttcaaagtt
     2821 ttcaccgatt tcagtgaaag catccgtgag agaggcggcg gcgaaaacag tgagagttgt
     2881 ttcttcggtt gtagaaacag gtatcggcgt ggtttga
//
LOCUS       DAOQJE010000455         2917 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_23218_length_2917_cov_6.534591, whole genome shotgun sequence.
ACCESSION   DAOQJE010000455 DAOQJE010000000
VERSION     DAOQJE010000455.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2917)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2917)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2917
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23218_length_2917_cov_6.534591"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1074
                     /locus_tag="PLT08_11715"
     CDS             <1..1074
                     /locus_tag="PLT08_11715"
                     /inference="COORDINATES: protein motif:HMM:NF024883.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HNE05180.1"
                     /translation="ELDEILNKTLGLVMNYMKVEAGEIFLLEEDKTTLRMMLHRGQAA
                     EAFWTHNIFRVGEGYPGVVAETCRPLICTDLANDPNFQREAVVKAGFHQVLSFPLVSG
                     ERVMGVMSIATRSKAAFEDRSVQMLTAVGAWAGLSIENARLHVNARRLAVLEERNRIG
                     MDLHDGIIQSIYGVGLALEGTKLLVDEDASSAKEKIDNAIDGLNQAIRDLRAYILDLR
                     PRQLGTEGVISGIKRLIAEYRANTFSEVTFMPPDSDLKELTQTQALALFHICQEALAN
                     AAKHAKAKNVQISLWTTEDRALLELHDDGKGFNTDEMSTFIGHGLANMQTRARSSGGD
                     IDITSVQGEGTTVFVWVPRGIKQ"
     gene            1559..2908
                     /gene="dnaA"
                     /locus_tag="PLT08_11720"
     CDS             1559..2908
                     /gene="dnaA"
                     /locus_tag="PLT08_11720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HNE05181.1"
                     /translation="MNAEQAWQTVLAQLQMDMPRASYDTWVRDTRPVAYENGMLTVGV
                     RNAYARDWLDSRLATTVNKLLIDTLNSKVSVKFVVSQSEDNASIADLETAPASIEITP
                     PEPKPRHVTLNPRYTFDTYVVGSGNRLAHAACQAVAEKPARAYNPLFLYGGVGLGKTH
                     LLHAIGNACHASGLNVLYVSSEEFTNDMINAIRSHTTQAFREKYRSADVLLIDDIQFI
                     AGKESTQEEFFHTFNTLHGQDKQIIVSSDRPPKSLVTLEERLRSRFEWGLTADIQAPD
                     LETRLAILRSKAERTGRNISDEILESIAQRVQSNIRELEGALNRIIAYADLSGASLTP
                     NLVEVALADLMPSRADIEPAHVVDLVARKFGLTSEKLLGRDRTKEVALPRQIAMYLLR
                     EEAKISFPQIGEVLGGRDHSTVMSAYEKIKEQIHADRRLEQDIVSIKQQLYNQSVNA"
BASE COUNT          808 a          683 c          660 g          766 t
ORIGIN      
        1 gaactggacg agattctgaa caaaacgctc gggcttgtga tgaactacat gaaagtagaa
       61 gccggagaaa tctttctttt agaggaagat aaaaccacac tgcgcatgat gttacatcgt
      121 gggcaagctg ctgaagcctt ctggacgcat aatatctttc gcgtagggga gggatatcct
      181 ggcgtagtgg ctgaaacttg ccgtccgctc atttgtacag acctggcaaa cgacccaaat
      241 tttcaacgtg aagcggtagt gaaggcggga tttcatcaag tattgagctt cccgcttgtt
      301 tctggcgaac gggttatggg ggttatgagc attgctaccc gttctaaagc tgcatttgaa
      361 gatagaagtg tccaaatgct cacggctgtg ggcgcttggg cagggctttc gattgaaaat
      421 gcacgtttac acgtcaatgc acgtcgttta gccgtgttgg aagaacgcaa tcgcattggt
      481 atggaccttc acgatggcat cattcaatcc atttatgggg ttggtcttgc gctggaaggt
      541 acaaaactcc tagtagatga agatgcttca tctgcaaagg agaaaattga taacgccatt
      601 gatggcttaa accaagcaat tcgcgattta cgagcttata ttcttgatct acgaccacgt
      661 caacttggta cagaaggcgt aattagtgga attaaacgct taattgctga atatcgtgcc
      721 aatacattct ctgaagtcac attcatgcca cctgattcag acctcaaaga actcactcaa
      781 acgcaagcct tggcactttt tcatatatgt caggaagcat tagccaatgc agccaagcat
      841 gccaaagcaa aaaatgtcca aatttcactt tggacaacag aagatcgtgc tttgcttgag
      901 cttcatgatg atggaaaagg cttcaacact gatgaaatgt cgacatttat tggtcatggt
      961 ctcgcaaata tgcagactcg tgcacgttca tcaggtggtg atattgatat aacatcggtt
     1021 caaggggaag gaacgaccgt atttgtatgg gttccacgcg gtatcaaaca ataaatcatg
     1081 attgttgccg aatcattgcc gggtgaatca caaaaactcg tgtttcacac tttttgattc
     1141 agataagttt tgcattatga ttctttaaga tagatatgcc aagcctgtgt cgaaaacccc
     1201 ttaaataccc atatatgggg gtcggttctt ggttcagggg cgttcttggc tgaaacgctt
     1261 gttctacaaa aaatgtagaa agaaagtaaa cagataacaa ttctttaaca ttgatgaatc
     1321 ttaaaaaaaa cctttttttt aacttagcgt aggttgaacg actgttacat atatgaatgt
     1381 tctgctagaa tatgaccacg ctgatataac ggatgtttgc ccgtacgatt gacatccgca
     1441 atccctaaag gatacttgac aaaacatgga tggaaattca agccgctgtt gggcggggtg
     1501 gtttcatcta cgcaaaaaac aagcaagacc ataaaatttt gtacattgag gaacgtttat
     1561 gaacgctgag caagcatggc agaccgtcct tgcacaactc caaatggata tgccgcgtgc
     1621 atcctatgat acgtgggtgc gcgatacccg tcccgttgcc tatgaaaacg ggatgttaac
     1681 cgttggggtt cgcaatgcct atgcccgtga ctggctcgat agccgccttg ccaccactgt
     1741 taacaagctt ttgattgaca cgctgaactc aaaagtttct gtgaaatttg ttgtttccca
     1801 atcggaagat aacgcctcta ttgcggacct cgaaactgct cctgcttcga tagagattac
     1861 cccgccggaa ccaaaacccc gccatgtaac gctcaatccg cgctatacat tcgataccta
     1921 tgtggtcggt tcgggaaacc gcctcgcaca tgcggcttgt caggcagtgg cagagaaacc
     1981 agcacgtgca tacaacccgc tcttcctgta tggaggcgtg ggactcggca agacccatct
     2041 cttacatgcc attggtaatg cctgtcatgc cagcggactc aacgtcctct atgtttcttc
     2101 tgaagaattc acgaatgaca tgatcaatgc cattcgttca cataccacgc aagcctttcg
     2161 tgaaaaatac cgttcggctg atgtgttgtt gatcgatgat attcaattta tcgccgggaa
     2221 agaatccact caggaagagt tcttccatac cttcaacact ttacatggac aagacaagca
     2281 aatcatcgtt tcgtctgatc gcccgcccaa atcccttgtg acgctggaag aacgcttgcg
     2341 ttctcgcttt gagtggggct taactgccga tattcaagcg ccagaccttg aaacccgcct
     2401 cgctattttg cgctctaaag ccgagcgaac cgggcgtaat atctctgatg aaattctcga
     2461 aagcattgct caacgcgtgc aatcgaatat ccgtgagctt gaaggggctc tcaatcgcat
     2521 cattgcctac gcagacctga gcggagcatc ccttactccc aatctcgtcg aagttgccct
     2581 tgccgacctc atgccctccc gtgcagacat tgaacctgca cacgtagtgg acttggttgc
     2641 tcgcaaattt ggtctcacct ctgaaaaatt actcgggcgc gaccgtacca aagaagtggc
     2701 attacctcgt caaatcgcta tgtatctctt gcgtgaagaa gctaagatct ctttcccgca
     2761 gatcggcgaa gtcctcggcg gacgcgacca ctcgactgtc atgtctgctt atgaaaaaat
     2821 caaagaacaa attcacgccg accgccgttt ggaacaagat attgtttcta tcaaacagca
     2881 gttatataac caaagcgtaa atgcataata aatacag
//
LOCUS       DAOQJE010000456         2910 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_23306_length_2910_cov_5.164273, whole genome shotgun sequence.
ACCESSION   DAOQJE010000456 DAOQJE010000000
VERSION     DAOQJE010000456.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2910)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2910)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2910
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23306_length_2910_cov_5.164273"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..679)
                     /locus_tag="PLT08_11725"
     CDS             complement(<1..679)
                     /locus_tag="PLT08_11725"
                     /inference="COORDINATES: protein motif:HMM:NF012568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HNE05182.1"
                     /translation="MTSNLQQSMLDAIELELKRQVGRLDQKRTAIFHEMLTYHMGWTG
                     EGAGSQATGKRIRPLITLLSVASINNKEEEKDDGIYWLHAKSMAAAVELVHNFSLVHD
                     DIQDNSELRRGRKTVWTKWGPPMAINVGDALFVIANQAVVDLKEHYPAEMVVRAAEIL
                     NECCLDLTRGQYLDMSYEERNDLSMDDYWPMIGGKTSALLSACTQVGALLGYANEEKI
                     ELYRQFGY"
     gene            complement(676..1299)
                     /locus_tag="PLT08_11730"
     CDS             complement(676..1299)
                     /locus_tag="PLT08_11730"
                     /inference="COORDINATES: protein motif:HMM:NF024811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HNE05183.1"
                     /translation="MKQNITAIIFDLGNVLIGWDAHRLYDRFFPTPAAVDRFLEEIRF
                     SEWNAKQDAGRPFQEGVAELSKEFPHYAELIQTYDTHWEESLTGVYEGTVEIVRELKQ
                     AGWALYLLSNFSVEKFDLIKHQHDFLNLFDDQVISGEHRTVKPEKAIFDITLKRINRK
                     AEECLFIDDSLPNIETARKMGFQTIHFQSPPLLREELKKLSIKGIDS"
     gene            1379..1801
                     /locus_tag="PLT08_11735"
     CDS             1379..1801
                     /locus_tag="PLT08_11735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE05184.1"
                     /translation="MSRLINPDSVGKERTRLTKSIVLCIRELAKQSQVTPETKDQAAY
                     IALALQAISEGIEASVAAWEKRDYWVKADKFRMEWMWAGQYAAKLKEAVLTDDWATIA
                     TMLPSIAQKFNKIEIAQNHRLGTPWTNAYKLLALHQKL"
     gene            complement(1857..2351)
                     /locus_tag="PLT08_11740"
     CDS             complement(1857..2351)
                     /locus_tag="PLT08_11740"
                     /inference="COORDINATES: protein motif:HMM:NF013052.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SET domain-containing protein-lysine
                     N-methyltransferase"
                     /protein_id="HNE05185.1"
                     /translation="MTTSTTTMDKLTIKTENKFRTLTTKQSYKKGEIISTIPSEDVRD
                     RPNRYTVQIGHDKHTHVGKLAALNHSCDPNVILDTENMEMVAARDIEKGEELFFFYPS
                     TEWQMDAPFICLCGSANCINVVAGARFLPLSTLERHYLNPHIREMMVELLNNTKNNLQ
                     KIKA"
     gene            complement(2474..>2910)
                     /locus_tag="PLT08_11745"
     CDS             complement(2474..>2910)
                     /locus_tag="PLT08_11745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE05186.1"
                     /translation="EKKLIWVSPFAGILAIALIYFMFIERWFYLRLEPVTGIHRIYQN
                     FYRIGKIFTGQISQAETSSEFLTNIIRTLEDIETIKNNTKRLNQTKKNAEALTSIYNR
                     SLFTNYRAKKADLVLAWNLWASLRRHLFVIKIFLLLNSKRSG"
BASE COUNT          811 a          577 c          661 g          861 t
ORIGIN      
        1 tatagccaaa ttgacggtat agctctattt tttcttcatt cgcgtagcca agaagagctc
       61 caacctgggt gcaggcagag agtagggcag aggtctttcc tccaatcatg ggccagtaat
      121 catccatcga aagatcgttg cgttcttcat aagacatatc caagtactga ccacgtgtta
      181 gatctaaaca acattcattc aaaatctccg cagcacgaac taccatctca gctggataat
      241 gttctttaag atcgactaca gcttggttgg caattacgaa caatgcgtct ccaacattaa
      301 ttgccatggg cggaccccat ttcgtccaca ccgttttccg tccgcgacgg agttctgagt
      361 tgtcttgaat atcatcatgc accaaagaga aattatggac aagttctact gcagctgcca
      421 ttgattttgc gtgtagccaa taaataccat catctttctc ttcctcttta ttattaatag
      481 aagctactga aagaagagta atcaaaggac ggatacgctt ccccgttgct tgtgaaccag
      541 caccctcacc tgtccaaccc atatgatagg tcaacatctc atggaagatg gcagtgcgtt
      601 tttggtccaa ccgccccact tggcgtttga gttccagttc aatggcgtcc aacatagatt
      661 gttgaagatt tgatgtcatg aatcgattcc tttaatggaa agttttttca gctcttccct
      721 taatagggga ggagactgaa agtgaatggt ttgaaacccc attttacggg cggtttcaat
      781 atttggaagt gaatcgtcga tgaaaagaca ttcttcagcc ttacggttaa tacgtttcag
      841 cgtaatgtcg aagatggctt tttcaggttt gacagttcga tgctcaccag agatgacttg
      901 atcgtcgaac aaatttagaa aatcgtgttg atgcttgatc aggtcaaatt tttctacgga
      961 gaagttactg agtagatata gcgcccaacc tgcttgtttt aattcacgaa cgatctcaac
     1021 agtcccttcg tagacaccgg taaggctttc ttcccaatgt gtatcgtagg tctgaatcag
     1081 ctcagcgtag tgagggaatt ctttcgataa ctccgcgacc ccttcttgga agggacgccc
     1141 agcgtcttgt ttggcattcc actccgagaa gcgaatctct tccagaaagc ggtcaacagc
     1201 tgccggggta gggaagaagc ggtcgtacag gcgatgggca tcccacccga tgaggacgtt
     1261 gcccaagtca aagatgatag ctgtgatatt ttgtttcata gatgccgcgc tattttatcc
     1321 gaagaagtta aatctggcgg tataatcgcg cggttgtaaa ctgacttgga gtagaacatt
     1381 gagccgacta atcaaccccg attctgtagg aaaagaacga actcgtttaa cgaagtctat
     1441 tgtgttgtgt attcgtgaac tggcgaaaca aagccaggta acgcctgaga caaaagatca
     1501 ggcggcgtat atcgccttgg cactgcaagc catttcagaa gggatcgaag cttctgtagc
     1561 ggcttgggag aagcgcgatt attgggtgaa agccgataag ttccgcatgg agtggatgtg
     1621 ggctggacaa tacgctgcca agttgaaaga ggctgtattg acagatgatt gggcaacgat
     1681 tgcgaccatg ttgccaagta ttgcgcagaa gttcaataag attgaaatcg cacaaaatca
     1741 tcggttgggc actccgtgga ccaatgcata caagctattg gcactgcatc aaaagttata
     1801 aggtaataga taaaaaaact ccgaagccta ggcttcggag tttttttatt gcatatttag
     1861 gctttgatct tttgaagatt gttcttggtg ttgttgagta gttcaaccat catctcgcgg
     1921 atgtgtgggt tgagatagtg acgctcgagt gtggaaagcg gtaagaagcg tgcaccggct
     1981 actacgttga tgcagtttgc tgaaccacat aagcaaatga agggcgcatc catttgccac
     2041 tcagtggagg gatagaagaa gaaaagttct tctcctttct caatatcgcg ggcagcaacc
     2101 atttccatat tctcggtgtc gagaataaca ttggggtcac aggaatgatt caacgcggcg
     2161 agtttaccaa cgtgtgtgtg tttatcatga ccaatttgca ccgtgtaacg attggggcga
     2221 tcacgcacat cttcactggg aattgtgctg atgatttccc cttttttgta actttgtttc
     2281 gttgttagag ttctgaattt attttcagtt ttgatggtca acttatccat cgttgttgtt
     2341 gaggttgtca ttgattatct cctttaaatt tttaaactaa tttattattg catcagtata
     2401 gcaataataa atttaaatac aagagttatt tgtatgaaat aaaaaatttt aaggtttgat
     2461 aaattatttg ttttcaacca gaacgcttgg agtttaataa caaaaatatt tttataacaa
     2521 acagatgacg tcgcagcgaa gcccataaat tccaagctaa gaccaaatct gcttttttag
     2581 cacgatagtt tgtaaacaaa gagcggttgt atatagacgt taatgcttct gcattctttt
     2641 ttgtttgatt gagtcttttt gtattattct tgatagtctc gatatcttcc agtgtgcgaa
     2701 tgatattggt aaggaattca ctggaggtct ccgcttgaga gatttgaccg gtaaaaatct
     2761 tgccgatgcg ataaaagttt tgatagatac gatgaatacc agtgacaggt tcaaggcgta
     2821 gatagaacca tctttcaatg aacatgaaat agataagtgc tattgcaagg atccccgcga
     2881 atggtgaaac ccaaattagc tttttttcta
//
LOCUS       DAOQJE010000457         2909 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_23320_length_2909_cov_7.029432, whole genome shotgun sequence.
ACCESSION   DAOQJE010000457 DAOQJE010000000
VERSION     DAOQJE010000457.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2909)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2909)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2909
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23320_length_2909_cov_7.029432"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..658
                     /locus_tag="PLT08_11750"
     CDS             <1..658
                     /locus_tag="PLT08_11750"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012424.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="dihydrolipoamide acetyltransferase family
                     protein"
                     /protein_id="HNE05187.1"
                     /translation="TTMRKSIAQHMVESKHTSPHVLTVMEADMSRVAKHRAANKAAFE
                     RDGVNLTFTAYFMMAIVAGLKAYPQTNSSWTDEGLLIHKNVNLGMAVSLGEDGLIVPV
                     IKKAEDLSLLAMARAVNDLANRARTKKLQPDDVKGGTFTLTNHGVSGSLFAFPVINQP
                     QAGILGVGAMQKRVVVIDDAIAIRPMVYMSFVFDHRILDGASADWFLAKVKETLENWA
                     "
     gene            662..988
                     /locus_tag="PLT08_11755"
     CDS             662..988
                     /locus_tag="PLT08_11755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE05188.1"
                     /translation="MKTLTGVLEAYYEQGWEGMIVFSFMPNDGNGLNDLITLRDGQLL
                     TIYDQNEATLWSGKIQFVKRGFFDKHKLKADIWSDSKQKGVSYADWMDWFWRQPPLKA
                     TLEFKD"
     gene            991..1467
                     /locus_tag="PLT08_11760"
     CDS             991..1467
                     /locus_tag="PLT08_11760"
                     /EC_number="2.1.1.100"
                     /EC_number="2.1.1.334"
                     /inference="COORDINATES: protein motif:HMM:NF018649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenylcysteine carboxylmethyltransferase
                     family protein"
                     /protein_id="HNE05189.1"
                     /translation="MSDKQKDSPAINKYIHPPIVAMFYIIVALLLGYLVPVLAGMSQT
                     LKNIGLGITFVGFLCGVGAFLEFRKARTTLDPHGSVKTVVTGGIYRFSRNPIYLGFLF
                     MVIGFPLAYGSLWGLVISPFFMVTLSRLVIEKEEAYLEKKFKEQYTSYKSKVRRWL"
     gene            1551..2207
                     /locus_tag="PLT08_11765"
     CDS             1551..2207
                     /locus_tag="PLT08_11765"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013866.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HNE05190.1"
                     /translation="MPIQTSDIQLSVNGKQVYAYLADGKGPGILLLHAWWGLKPFFKQ
                     VCDRIAEQGFTVLAPDLRGGQIAKTIDEAKALMEKSDGQFTGDTVFTAKAHLQTLVKG
                     KLGLIGFSMGAAWSLIAAAYKPEDFGAVVLFYGNEGVDYGKITAKVLGHFCEVDEWEP
                     TEFVDNTFAEFAKAGVDATRHTYPGTAHWFVEEDRPEYDAAAAQLAWSRTYDFLKKTL
                     "
     gene            2324..>2909
                     /locus_tag="PLT08_11770"
     CDS             2324..>2909
                     /locus_tag="PLT08_11770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HNE05191.1"
                     /translation="MSENFDVVVIGAGPAGYVAAIRASQLNQKVAIVDKQWMGGVCLN
                     VGCIPSKSLLKNAEVAHTLRHRAKDFGFSFDNLKLDYSVAFKRSRSNSDRLVKGIGFL
                     MKKNNIAVFMGAAQFKAKDTLTVTDKDGKVTELKAKNIIVATGASAATLPGVTIDGKK
                     IVTYTEAIMQDTLPQSAVIVGAGAIGVEFATIWNS"
BASE COUNT          750 a          688 c          740 g          731 t
ORIGIN      
        1 caccaccatg cgcaaatcga ttgcgcagca catggtcgag tccaagcata cctcgccgca
       61 tgtattgacg gtgatggaag cggatatgtc tcgggtggca aagcatcgtg ccgcgaacaa
      121 agccgctttc gagcgcgacg gggtcaacct caccttcacg gcgtatttca tgatggcaat
      181 tgtggcaggg ctgaaagcct atccgcagac caattcttcg tggacggatg aaggcttgct
      241 cattcataag aatgtcaact tgggtatggc ggtctcactg ggtgaagatg gtttgatcgt
      301 gccggtcatc aagaaagccg aagacctgtc cctgctggcg atggcacgtg ctgtcaacga
      361 tctcgcaaat cgtgcgcgta ccaagaaact acaaccagat gatgtcaaag gcggcacatt
      421 cacattgacc aaccacggcg taagcggatc gctgtttgca ttcccggtca tcaatcaacc
      481 gcaagcggga attcttggag taggcgcgat gcaaaaacga gttgtcgtga ttgacgatgc
      541 catcgcgatc cgaccgatgg tgtatatgtc cttcgtcttc gaccaccgca tcttggatgg
      601 cgcttcagca gattggttcc tggcgaaggt caaagagacc ttggagaatt gggcataaca
      661 attgaaaacg ctgaccggag ttcttgaagc ctattacgaa caaggctggg aaggtatgat
      721 tgtcttcagt ttcatgccaa atgatggaaa tggactgaat gacctcatca ccttgcgaga
      781 cggtcaacta ctaaccatct acgatcagaa tgaggctaca ctctggtctg gaaaaatcca
      841 gttcgtaaag cgcggctttt ttgacaagca caaactcaaa gcggatatct ggtcagactc
      901 aaaacaaaag ggcgtctctt atgccgattg gatggattgg ttctggcgtc agccgccatt
      961 gaaggcaaca cttgaattta aggattgaaa atgagcgata aacaaaaaga cagcccggcg
     1021 atcaataaat acatccaccc acccatcgtg gcaatgtttt acatcattgt tgcattattg
     1081 ctgggttatc tcgtccctgt attggcgggc atgtcacaaa ccctaaaaaa tatcgggctg
     1141 ggtatcacct ttgttggttt tctctgtgga gttggtgcgt ttcttgaatt tcgaaaagca
     1201 cgcaccacgc tcgacccaca tggttccgtc aaaaccgtgg tgacgggtgg aatttatcgt
     1261 ttttcacgta accccattta tctgggcttc ctttttatgg tgatcggctt tccacttgca
     1321 tatgggtcgt tgtgggggct tgtcatctca ccatttttca tggtcacact ctcgcggctt
     1381 gtcatagaga aagaagaagc ctatctggag aaaaaattca aagagcaata caccagctat
     1441 aagtccaaag tgaggcgttg gttgtaaatc ccaaaggcgg cgaaagtcgc ctttttcttt
     1501 tgcttgtatt ttagaacaaa tgtacttata atcatttgga ggagattgcc atgcctattc
     1561 aaacatctga cattcaatta agtgtgaacg gaaaacaagt ctatgcctat cttgcggatg
     1621 ggaaaggtcc cggcattttg ttattacatg cttggtgggg actgaagcca ttcttcaaac
     1681 aagtctgtga ccgcattgcc gaacaaggct ttactgtgct cgcccccgat ctacgtggcg
     1741 gacaaattgc gaaaactatt gacgaagcaa aagccttgat ggaaaaaagc gatggacaat
     1801 ttaccggtga taccgttttt acggcgaagg cacatttgca aacgctggtg aaaggcaaac
     1861 ttgggttgat cggtttttcg atgggcgcgg catggtcact gattgcggct gcatacaaac
     1921 cggaggactt tggtgcggtc gtgcttttct acggcaacga aggcgtggac tatggcaaga
     1981 tcactgccaa agtgctgggt catttctgtg aggtggacga atgggagccc accgaatttg
     2041 tggataacac ctttgcagag ttcgccaaag ccggcgtgga tgcgacgcgt cacacctacc
     2101 cgggaactgc ccactggttc gtggaagagg atcgccccga atacgatgcc gctgccgcgc
     2161 agttagcatg gagccgtaca tacgactttt tgaagaagac tctctaagaa cgactcaatt
     2221 tgaaaattgc ttataaaact ccaacacctc ccggcatggg aggtgtttct ttagtagtaa
     2281 aatcaagccg taatttcact ttcttaaacg taatggagtt tctatgtccg aaaatttcga
     2341 tgttgttgtg atcggtgcgg gacctgccgg ctatgtagct gccatccgcg cttcacagtt
     2401 gaaccagaaa gttgccattg tcgacaagca atggatgggc ggggtttgtt tgaatgtagg
     2461 ttgtatccct tcaaagtccc ttttgaaaaa tgctgaagtg gcacatacct tgcgtcaccg
     2521 cgccaaggat tttggtttct cgtttgataa tctcaagctg gattacagtg tggcgttcaa
     2581 gcgctcacgc tccaactcag accgccttgt aaaaggcatt ggcttcctca tgaagaagaa
     2641 taatatcgct gtcttcatgg gcgcggcaca attcaaagcc aaagataccc ttaccgtgac
     2701 cgataaagac ggcaaagtga ccgagctcaa agcgaagaac atcatcgttg ctactggcgc
     2761 cagtgcagcc accctgcccg gcgtgaccat cgacggcaag aaaattgtca cctacaccga
     2821 agccattatg caagataccc tcccccagtc tgcggtgatc gtgggtgcgg gcgccatcgg
     2881 cgtggaattt gccaccatct ggaattcct
//
LOCUS       DAOQJE010000458         2904 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_23394_length_2904_cov_2.705862, whole genome shotgun sequence.
ACCESSION   DAOQJE010000458 DAOQJE010000000
VERSION     DAOQJE010000458.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2904)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2904)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2904
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23394_length_2904_cov_2.705862"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1219
                     /locus_tag="PLT08_11775"
     CDS             <1..1219
                     /locus_tag="PLT08_11775"
                     /inference="COORDINATES: protein motif:HMM:NF013704.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006306 - DNA methylation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DNA methyltransferase"
                     /protein_id="HNE05192.1"
                     /translation="KAVESSFHSNGITQGQVIYDPFMGSGTTNLVAKKLAINSYGVEA
                     HPFVFRITRTKMNWEISRDEIAHALRDVEAQVKKRKQNFNGTDIQTFLEKEFPELVLK
                     CYENNTLLDLLFIRNAIAKGNFSEAIKDLFFVGLTSILRQVSTAATGWPYIAPNKQKT
                     SSLNKNAMFEFSRQIKKMADDIQVTVQEANKYYGNYFHNIFNADSRKTQELIPSSCAD
                     HVFTSPPYLNNFDYADRTRLELYFWGEAKNWGDISDNIRTKLITSATTQISRDDPRYN
                     LSEGLQEASPDVFDFLRKSVSQLGEIRLTRGGKKSYDHLVSGYFNDMFEIISEVYRIL
                     KPNTKAVFVLGDSAPYGVHIPTDDLIGKIGLGVGFSDYRIDVLRERGGKWKDNPQRHN
                     VLLRESIVTLLKA"
     gene            1223..2050
                     /locus_tag="PLT08_11780"
     CDS             1223..2050
                     /locus_tag="PLT08_11780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE05193.1"
                     /translation="MADDIQNPGSALGEAIGAEMEKALNDFLTNLVESRGNHFLSKSP
                     VKNKNGTPKKLLMYDNFGTAYNIDAVIANESMQPIILIESKYIRYKKHNRDKGSWVCT
                     AHPAVRRRYDSIRSSIAVLAGNWSSTSLTMMKSFDINIFLVPFTKICDLLEKHKINFD
                     WGEKDREMAKDAWKKYSALTNAQKYEIGAEMVNVVKDELEILVLSILDDTLEREVNRI
                     TIELHSNLGEVKAYEFSTVDEAVEFLNTAELKEVFISSDSLTLFDPPPVIDDDDDDE"
     gene            2263..2760
                     /locus_tag="PLT08_11785"
     CDS             2263..2760
                     /locus_tag="PLT08_11785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE05194.1"
                     /translation="MEKVSIIFSVLIHSNEFMYLFWGILGVLNLRSGSFLLKAKEVTE
                     KQLKEISPAHEHVMYDYTFYSAYTLALTFVLTWIVTSSFNDWALENYGRNFYPSFVFG
                     LAIYGVFTALFALSRGVYPIVRPKMLVFFIYDDVKLINSAAKFHIIISVVLFSMSVIV
                     FFATV"
BASE COUNT          916 a          532 c          597 g          859 t
ORIGIN      
        1 taaagcggta gagtcgagtt ttcattccaa cggcattaca caaggtcaag ttatttatga
       61 cccgttcatg ggttcaggaa ctacaaattt agtcgcaaaa aaactagcga ttaactctta
      121 tggtgttgaa gcacatccct ttgtttttag aattactaga actaaaatga attgggaaat
      181 tagcagggat gaaatcgctc atgctcttcg agatgtggaa gcgcaagtaa aaaaacgaaa
      241 gcaaaatttt aacggaacag acattcagac atttttggaa aaggaatttc cagaattggt
      301 tttgaagtgt tacgaaaata acactttatt ggatttgctt tttatccgta atgcaattgc
      361 aaaaggtaat ttttcagaag cgattaaaga ccttttcttt gttggtctta caagtatttt
      421 gcgtcaagtt tcaacggcgg ctactggatg gccatatatc gccccgaata aacagaaaac
      481 cagttctttg aataaaaatg ctatgtttga attttctcgc caaataaaaa aaatggcgga
      541 tgatattcaa gtaacagttc aagaagccaa caaatattat ggcaattact ttcataatat
      601 tttcaatgcc gattcaagaa agactcaaga gttaatccct agttcatgtg ctgaccatgt
      661 ttttacttca ccgccctatt tgaataactt tgattatgca gaccgcaccc gtttagagtt
      721 atatttttgg ggcgaagcaa aaaactgggg cgatatttcc gataatattc gcacaaagtt
      781 aattacttct gcaactacgc aaatttcaag agacgaccca agatacaact tatcggaagg
      841 tttgcaagaa gccagcccag atgtttttga ttttttgaga aagtcagttt cacaattagg
      901 tgaaattaga ttaactcgtg gcggaaaaaa gagttatgac catcttgtgt caggttattt
      961 caatgatatg tttgaaatta tctctgaagt ttacaggata ttaaaaccta atactaaagc
     1021 cgttttcgtt cttggcgatt ctgcgcctta tggcgttcac attcccaccg atgacttaat
     1081 tggaaaaatt ggtttaggtg tgggattttc ggattatcga attgacgttt taagagaacg
     1141 tggcggaaag tggaaagaca atccacaaag gcataatgtt ttacttcgtg aatccatcgt
     1201 tacattatta aaggcataaa tcatggcaga tgacatccaa aaccctggga gcgcacttgg
     1261 cgaagcaatt ggcgcagaaa tggaaaaagc gttaaacgat tttctgacaa atcttgttga
     1321 aagtcgtggt aatcattttt tgagcaaaag ccccgttaag aataaaaatg gaacgccgaa
     1381 aaaacttttg atgtacgaca attttggtac tgcatacaac attgacgctg taattgccaa
     1441 tgaaagtatg caaccaataa ttttgattga gtcaaaatac atccgataca aaaagcataa
     1501 tcgagataaa ggcagttggg tttgtactgc tcatcctgcc gtaagacggc gatacgacag
     1561 tatcagaagt tcaattgctg tgttggcggg taattggagt tcaacgtctt tgacaatgat
     1621 gaaaagtttt gacatcaata tttttcttgt cccattcacc aaaatttgtg accttttgga
     1681 aaagcacaaa atcaattttg attggggcga aaaagaccga gagatggcaa aagacgcatg
     1741 gaaaaaatat tcagccctta caaatgctca aaagtatgag attggcgcag aaatggtgaa
     1801 tgtcgtcaaa gatgaattag agatacttgt cttaagtatc cttgacgata cgcttgaaag
     1861 agaagttaac agaattacga ttgaattgca ctcgaatctt ggcgaagtga aagcgtatga
     1921 attttcaaca gttgatgaag ccgtagaatt tttgaataca gcagaattga aagaagtatt
     1981 tatttcaagc gattcgctca cgttatttga cccaccgccc gttatagacg atgacgatga
     2041 tgatgaatag aaaaacttct ctaaagtcaa aagcaggcta acaaagcgtg cacctgacgt
     2101 gtgggattct gcggcatttt cgagcatttt cctcgcttcg agtttttcct gctcccaaag
     2161 cgaatccacg cccgcccaca cgcaggtaac gcaaaccgtt gggcgctggc gcgaaaaaag
     2221 aaaattgttt tggcttgctg acaaaagtag ttgcgggtta tcatggaaaa agtctcaata
     2281 atattttcag tcttaataca cagcaatgaa ttcatgtatc tattttgggg aattcttgga
     2341 gttctcaatt tgaggtcagg ttcattcctc ttgaaagcaa aggaagtaac tgaaaaacag
     2401 ttaaaagaaa tatcacctgc tcacgagcat gtcatgtacg attacacatt ttatagcgcc
     2461 tacactcttg cgcttacctt tgtattaaca tggatagtga cctccagctt caatgactgg
     2521 gctctggaaa attatggaag aaatttctac ccatcttttg ttttcggtct cgcaatttac
     2581 ggggtattca cagctctgtt tgccctgtct agaggtgtat atcccatcgt tagaccaaaa
     2641 atgttggtat tttttattta tgacgatgtg aaacttatta atagtgccgc aaaatttcat
     2701 atcattattt cagtcgtgtt attcagcatg tctgttatag tcttttttgc aacagtttag
     2761 gtaaaagcaa aggtgtacaa tatccaaaat tgcgcccaac aaagcgtgca cctgacgctg
     2821 gggattctgc ggcaatccca agcatttttc acgccttagc atttttctgg ctggacggct
     2881 tcgccgtccc cgccccagcg cagg
//
LOCUS       DAOQJE010000459         2901 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_23420_length_2901_cov_5.092410, whole genome shotgun sequence.
ACCESSION   DAOQJE010000459 DAOQJE010000000
VERSION     DAOQJE010000459.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2901)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2901)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2901
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23420_length_2901_cov_5.092410"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..575)
                     /locus_tag="PLT08_11790"
     CDS             complement(<1..575)
                     /locus_tag="PLT08_11790"
                     /inference="COORDINATES: protein motif:HMM:NF012792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNE05195.1"
                     /translation="MSSLSNIAIKLIPASHFSIDELTGIYNQTRVDYMVPMPMNSARL
                     MEYIEFYDVDLDRSVVAIEDGELRGVAMLGVRAERTWVTRLGVIPSTRRGGVGEALMQ
                     SLLEQSARLGIDFTMLEVIKNNVPAHRLFLKLGFYEIGELLILRRAPSKKLVEPVIAD
                     ARRLERYEALDLVGYDRGTQPWTNQSESMSHA"
     gene            complement(585..956)
                     /locus_tag="PLT08_11795"
     CDS             complement(585..956)
                     /locus_tag="PLT08_11795"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNE05196.1"
                     /translation="MTRILIVEDVPDNVELARKILTSRGYEVIHAINAEMGYNLAIQE
                     RPDLVLLDLGLPDYDGLTLAGQIRENPAFERLPIIAFTAWPQETAKNMAESYGCNGFI
                     SKPIIRVNEFIAQVESFLLND"
     gene            1134..2267
                     /gene="sucC"
                     /locus_tag="PLT08_11800"
     CDS             1134..2267
                     /gene="sucC"
                     /locus_tag="PLT08_11800"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-forming succinate--CoA ligase subunit beta"
                     /protein_id="HNE05197.1"
                     /translation="MKLHEYQSKNIFSKFGIPIPKGRVATTAQEAKQIAEELGGRVVI
                     KSQVLVGGRGKAGGVKLAKDPAEAEQLATQILGMEIKGLPVRKVLVDEASAIDQEIYF
                     AITDDRAAKKPVLIASAAGGVDIEEVAAKTPEKIIKVNIDPLLGLREYQARDVAVAID
                     LPRDYWKDFTKIAMGLWHVYQSTDASLAEINPLVITKDKKMIALDGKMMIDDNALFRQ
                     SDLAEIRDTDEDAPAEIEARKYGLSFIKLDGNIGCMVNGAGLAMTSMDVIKLFGGDPA
                     NFLDVGGGAGAEKVAAAMRIILTDPNVKAVLFNIFGGITRCDEVAKGILVAMDEVKPK
                     VPMVVRLVGTNAEEGRRLLENANMITAETLADAAKKAVAAAKG"
     gene            2273..>2901
                     /locus_tag="PLT08_11805"
     CDS             2273..>2901
                     /locus_tag="PLT08_11805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate--CoA ligase subunit alpha"
                     /protein_id="HNE05198.1"
                     /translation="MSILVDKNTRLLVQGITGNEGLFHTTQMAAYGTNIVGGITPGKG
                     GEWVLDGKIPVFDSVKSAKDATDANATIIFVPARFAPDAMFEAADAGIPLVICITEGV
                     VIHDMMRVRNYLDQKKVRLIGPNCPGLLTPGEAKVGIIPGNIAIPGNVGVVSRSGTLT
                     YEVLYAMKNLGMGASTCVGIGGDPVNGTNFTDVLEMFEHDPKTEKVIMIG"
BASE COUNT          772 a          751 c          681 g          697 t
ORIGIN      
        1 gcatggctca tcgattcgga ttgattcgtc catggttgag tcccacggtc atatcccaca
       61 agatccagtg cttcatatcg ttccaatcga cgtgcatctg ctattacagg ttccactagt
      121 tttttagatg gcgcacgcct aagaatcagc aactccccaa tttcataaaa cccaagtttc
      181 aggaacaatc gatgggcagg aacgttattt ttaataactt ccagcatggt gaagtctatg
      241 cccagtctgg cagattgctc cagcaagctt tgcatcaagg cttcaccaac gccgccccgt
      301 cgtgtggacg ggattacacc taaacgggtg acccatgtcc gctcggcacg gacccccaac
      361 atagctacac cccgtaattc tccatcttca attgcaacaa ccgaacgatc taaatctaca
      421 tcataaaact caatatattc cataagacgc gcagagttca tgggcatggg caccatataa
      481 tctacgcgag tttggttgta gatgccagtc aattcgtcaa tggaaaaatg actagcgggg
      541 attaacttaa tggcaatatt tgataaagaa gacattctta agggttagtc gtttaagagg
      601 aaactctcta cttgagcaat aaactcattc acacgaataa taggtttact aataaaacca
      661 ttgcagccat agctttctgc catgtttttt gctgtctctt ggggccatgc agtaaaggca
      721 atgattggca gtcgttcaaa ggctgggttt tcacgaattt gcccagctag ggtcaaaccg
      781 tcataatcgg gcaaccctaa atccagcaac acaaggtctg ggcgttcttg tatggcaagg
      841 ttgtatccca tttcagcatt tatggcatga ataacttcat accctctcga agtaaggatc
      901 tttcgagcta attccacatt atcgggcaca tcttctacaa ttaggattcg cgtcatagtt
      961 acctttggta aaacttaagg tcaaagcaat gttaccactt gcagaaaagg attccattgc
     1021 aatttatcgc tagaagttct tggcagaaat atagtatttt acaacccgtg tctatttcga
     1081 agtggtataa tgccgatgct ttttagcaaa aatatactct ccgagggaga agaatgaaac
     1141 tccacgaata ccaatccaaa aatatttttt cgaaatttgg catcccaatc cccaagggac
     1201 gcgttgcgac taccgctcaa gaggcgaagc agatcgccga agagcttggt ggtcgcgttg
     1261 tcattaaatc gcaggtgttg gtaggcggac gcggcaaagc tggcggtgta aagctcgcca
     1321 aggaccccgc cgaagccgaa cagctcgcca ctcagattct gggcatggag atcaaaggtc
     1381 tccccgtgcg caaagtcttg gtggatgaag cctctgccat cgaccaggaa atctacttcg
     1441 cgatcaccga tgaccgcgcc gccaagaaac cggttcttat tgcctctgct gccggtggtg
     1501 tggacattga agaagttgcc gccaagaccc ccgaaaaaat cattaaagta aacattgacc
     1561 ccttgcttgg cttacgcgaa tatcaggctc gtgacgttgc cgttgccatt gaccttccac
     1621 gtgattattg gaaagatttc accaaaatcg ccatggggct ttggcatgtc tatcaaagca
     1681 ccgacgcctc cctcgcagaa atcaacccgt tggtgatcac caaagacaag aaaatgatcg
     1741 cactcgacgg caaaatgatg atcgatgaca atgcgctctt ccgtcaatca gatttggctg
     1801 agattcgcga taccgatgaa gatgcacctg ctgaaatcga agctcgcaag tatggtctgt
     1861 ctttcattaa gttagatggc aatatcggct gtatggtcaa tggcgcaggt ctggcaatga
     1921 ccagcatgga cgtaatcaaa ctctttggtg gcgaccccgc caacttcctc gatgttggag
     1981 gcggtgctgg cgcagagaaa gtcgctgctg ccatgcgcat catcctgact gacccgaacg
     2041 tcaaagcagt gcttttcaat atcttcggcg gcatcacccg ctgtgacgaa gttgccaagg
     2101 gcattctcgt tgccatggac gaagtcaagc cgaaggtgcc gatggtcgtt cgccttgttg
     2161 gaacgaatgc agaagaaggt cgccgtttgc ttgaaaatgc aaacatgatc actgccgaaa
     2221 ctctcgccga cgccgccaag aaagccgtcg ctgcggcaaa gggataatac aaatgagcat
     2281 tttagtagat aaaaataccc gccttcttgt gcaaggcatc actggcaacg aaggcttatt
     2341 ccacaccacg cagatggctg catatggcac caatatcgtc ggtggaatca cccccggcaa
     2401 gggcggtgaa tgggtcttgg atggaaagat ccctgtcttt gattcggtca aatctgccaa
     2461 agacgcaacc gatgcaaacg caaccatcat cttcgtccct gcccgcttcg caccagacgc
     2521 catgttcgaa gccgctgatg caggcattcc gctcgtcatc tgcattaccg aaggtgttgt
     2581 catccacgac atgatgcgcg tacgcaacta tctcgaccaa aagaaggtcc gccttattgg
     2641 tccgaactgc cccggtctgt tgacgcccgg tgaagccaag gtcggcatca tccccggcaa
     2701 tatcgccatc cctggcaatg tcggcgtggt ctcccgctct ggcacattga cctatgaagt
     2761 gctctatgcc atgaagaacc tcggcatggg cgcatcaaca tgcgtcggca tcggtggtga
     2821 cccggtcaac ggcaccaact tcaccgatgt gcttgaaatg ttcgaacacg accccaagac
     2881 cgaaaaggtc atcatgatcg g
//
LOCUS       DAOQJE010000460         2894 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_23523_length_2894_cov_6.101092, whole genome shotgun sequence.
ACCESSION   DAOQJE010000460 DAOQJE010000000
VERSION     DAOQJE010000460.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2894)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2894)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2894
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23523_length_2894_cov_6.101092"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..371
                     /locus_tag="PLT08_11810"
     CDS             <1..371
                     /locus_tag="PLT08_11810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE05199.1"
                     /translation="SHTYIPDFGKALVILGERDEADGLAWHVPNDNPRVTQREMAKLI
                     ADELGLPLKVSAIGKTMMAIGGLFIPEAKESVEMMYEFEKPFIVDSSKFEKTFGVKAT
                     PMKDAIKETVAWYKSHPHTK"
     gene            395..850
                     /locus_tag="PLT08_11815"
     CDS             395..850
                     /locus_tag="PLT08_11815"
                     /inference="COORDINATES: protein motif:HMM:NF021495.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2214 family protein"
                     /protein_id="HNE05200.1"
                     /translation="MVIATSLMATLHHIAAFTMTACLVYEFIALRKSISSVEVRRIQQ
                     VDLWYGISAGVLLVAGLLRVFIFEKGASYYGANPLFWVKMALFALVGILSIYPTIRYI
                     RWGNVTDDKKLELPDDEYKRIRLLLNLEVIGIALILFAAPAMARAIGMN"
     gene            complement(883..1974)
                     /locus_tag="PLT08_11820"
     CDS             complement(883..1974)
                     /locus_tag="PLT08_11820"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNE05201.1"
                     /translation="MRVAIVHEWLSTFGGSERLLVQLLQLFPHADIFALTHFPKNFQH
                     TELKDIRVHTTFLQNLPNIERQYRKLLPIMPLAIESLDLSAYDLVLSLSHAVAHGVKT
                     HKNQAHISYISTPMRYAWHMRDDYLKLHGLTNPLVRLAANGTLSLLRRWDTFASARSD
                     SLIANSQWTASHIHEAWGRDSQVIYPAVDISRFTPSTQRENYYLLVSRLVPYKLGIEI
                     VKAFNSLKLPLIIVGDGPELQHIQEIAKENITVMGYQPDAVVTELMNKAKAFVYMATE
                     DFGIAMVEAQAAGCPVIAYYKGGASEIIRDGETGLLFKEQTAVSLAEAVTTFEAMNLN
                     SKAASDNAARFSSERFRREFLDHVHKFLE"
     gene            2068..2751
                     /locus_tag="PLT08_11825"
     CDS             2068..2751
                     /locus_tag="PLT08_11825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE05202.1"
                     /translation="MNNFFSRFFRIGAQLFFALTIILMPFRLRLVLWTRPFFPVYSDY
                     TDFLLFASDLGVLFILGLWASSLIVSPRKVKVGNPFIWYLLLGITLSAWMSILGSVDP
                     IITRYHAVRFVFLLLFYLYIVNEIVSIAWVIIPVAIQAVIQAPIAIAQFVKQSSLGLQ
                     VLGEHTLDPLVLGTSIIPVNGERILRAYGLTDHPNIAGGCLTFALVLLFSVFFYGKKW
                     QFTLKIFQQ"
BASE COUNT          727 a          575 c          718 g          874 t
ORIGIN      
        1 attcacatac ttacattcct gattttggga aggcgttagt tattcttggt gagcgcgacg
       61 aagctgatgg gctcgcctgg catgtgccga atgacaatcc gcgtgtgacc cagcgtgaga
      121 tggcaaagtt gatcgcagac gagttgggac tacctctcaa ggtcagtgcg ataggcaaaa
      181 ccatgatggc gatcggcgga ttgttcatcc ctgaggcgaa agagtctgta gagatgatgt
      241 acgagttcga aaagccgttc atcgttgatt caagcaagtt tgaaaagact tttggtgtga
      301 aagccacacc gatgaaagat gcaataaaag aaaccgtggc ttggtacaag agccacccac
      361 atacgaagta acaaaaacta ttaggagaga gagtatggtg attgcgacat ctttaatggc
      421 aaccttgcat catatagcag ctttcacaat gactgcctgt ttggtttatg agtttattgc
      481 tttgcgtaaa tcaataagtt ctgtagaagt gcgccgtatt cagcaagtgg atttatggta
      541 tggcatctca gcaggtgtgt tattggtggc tgggctattg cgagtgttca tttttgaaaa
      601 aggggcaagt tactatggcg caaacccgtt gttctgggtg aagatggctt tatttgcgct
      661 ggttggaata ctttctattt acccaactat ccgttatatt cgctggggaa atgtaacgga
      721 tgataaaaaa ttagagttgc ccgatgatga gtacaaacga atccgcttgc tattgaattt
      781 ggaagtgatc ggtattgcac taattctatt tgcagcgcca gcaatggcac gtgccatcgg
      841 catgaactga cacccttcca gctttacagt agactgaagc gcttattcta aaaacttgtg
      901 gacgtgatct aaaaattccc gtctaaagcg ttcagatgaa aagcgagccg cattgtctga
      961 tgcggctttg ctatttaagt tcattgcctc gaatgtagtt acagcctcgg ctaatgatac
     1021 ggcagtctgt tctttgaata aaagacccgt ctctccgtca cggataatct ctgatgcgcc
     1081 acctttgtaa taagcaataa cggggcaacc tgccgcctgc gcttcgacca tggcaatgcc
     1141 gaaatcttca gttgccatgt acacaaatgc cttggctttg ttcatgagtt ctgtgacaac
     1201 cgcgtcgggc tgatatccca tcacggtgat attttctttt gcgatttctt gaatatgctg
     1261 aagctcaggt ccatcaccga cgatgatgag cggtaacttc aacgagttaa aagctttgac
     1321 aatttctatg cccaatttat atggcacaag gcgcgatacg agtaggtaat aattttcgcg
     1381 ttgtgtagag ggtgtaaaac gactgatatc gactgctggg taaatcactt gcgaatctcg
     1441 tccccatgct tcatggatgt gtgatgctgt ccattgagag ttggcaatca gcgaatcaga
     1501 tcgagcagag gcaaaggtgt cccaacggcg gagcaggctc aaggtcccgt ttgcggcgag
     1561 gcggacgagg ggattggtca agccgtgcag tttcaaatag tcatcccgca tgtgccaagc
     1621 atagcgcatc ggagtcgaga tgtaagagat gtgagcttgg tttttgtgcg tttttactcc
     1681 gtgggctacg gcatgagaga gcgaaaggac aaggtcgtat gcacttaaat ccaaagactc
     1741 aatggcaagc ggcatgatgg gtaagagttt gcggtactgg cgctcgatgt ttggcagatt
     1801 ctgtaaaaag gttgtgtgga cccgaatgtc ttttagttct gtatgttgaa agtttttagg
     1861 aaaatgagtc agcgcgaaaa tatcggcgtg cggaaaaagc tgcaacaatt gaacaaggag
     1921 tcgctcgctg ccaccgaagg tgctcagcca ttcatggacg atagcaactc tcatttgatt
     1981 ctcaatattt gtgtgggaat tatcagaaac ataaccggcg acagattata attgtaaaat
     2041 tggattgctt aggtcttatt tgttgttgtg aataatttct tctcccgctt ttttagaatt
     2101 ggtgcgcagc tcttctttgc gttgacgatc atcctcatgc cgtttcggtt gcgactggtg
     2161 ttgtggacgc gtccattttt tccagtgtat tctgattaca ccgattttct tttatttgcc
     2221 agtgaccttg gggttttatt tatattgggc ctttgggcat cgtcgttgat agtttcacct
     2281 cgcaaagtga aggttggaaa tccttttatc tggtatctgt tattgggaat taccctttcg
     2341 gcatggatgt caattttagg cagcgttgac ccaattatta ctcggtatca tgccgttcgc
     2401 tttgtatttt tgcttttgtt ttatttgtat attgtgaatg agattgtttc tatcgcttgg
     2461 gtgattattc ctgtcgcaat tcaggcagtg atacaagcgc ctattgcaat tgctcaattt
     2521 gtgaaacagt cttcgcttgg gctgcaagta ctcggtgaac atacccttga cccgcttgtg
     2581 ttaggtacga gcattatccc cgtcaatggc gagcggatct tgcgcgctta tgggcttaca
     2641 gatcacccaa atattgcagg cggatgtttg acatttgccc ttgttctctt attttctgtt
     2701 ttcttttatg gcaaaaaatg gcagtttaca ctgaaaatat ttcagcagta atggctgctt
     2761 aatcaatatt tcggcaattt ccgattttct ataaagtcca atcaattaca gttttgcctt
     2821 ggctaccaag taactgattt gtttttgaaa aatgcttgca gccaaagaag gcaccaccag
     2881 aaagtgggga ggga
//
LOCUS       DAOQJE010000461         2886 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_23621_length_2886_cov_6.533734, whole genome shotgun sequence.
ACCESSION   DAOQJE010000461 DAOQJE010000000
VERSION     DAOQJE010000461.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2886)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2886)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2886
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23621_length_2886_cov_6.533734"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            115..954
                     /locus_tag="PLT08_11830"
     CDS             115..954
                     /locus_tag="PLT08_11830"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase"
                     /protein_id="HNE05203.1"
                     /translation="MSYAHPEYLVETDWVAERLNDPNIRIIESDEDPLLYAIGHIPGA
                     AQVDWFSTLQHPLRRDFLTKEQFEQVASKLGITNDTTVIFYGDKSNWFACYALWLFQY
                     YGHQNVKIVNGGRAKWEKEGRPLVKEVPTFTATTYKAKEADKSIRAFRDDVFKQVDSK
                     KPLVDVRSPKEYTGELLHMPNYPQEGATRGGHIPGAVSIPWAQAVNEADSTFKTAEEL
                     KSLYEGKNIKSDGEIIAYCRIGERSSLTWFVLKYLLGYPTVKNYDGSWTEWGNLVDAP
                     IEK"
     gene            1018..1449
                     /locus_tag="PLT08_11835"
     CDS             1018..1449
                     /locus_tag="PLT08_11835"
                     /inference="COORDINATES: protein motif:HMM:NF014688.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SufE family protein"
                     /protein_id="HNE05204.1"
                     /translation="MTLPNKLQEIVDDFASMSREEKLETLIAYAESMPELPERFHAER
                     EKMEAVPECMTPVFIAAEKDSNNGIVFHLDIPKQSPTVRGLASILTNGLNGCSLAEII
                     SVPTEFYLPMNLQEAVSQQRINGFMGVLAHMKQVAVRVGSG"
     gene            complement(1499..1903)
                     /locus_tag="PLT08_11840"
     CDS             complement(1499..1903)
                     /locus_tag="PLT08_11840"
                     /inference="COORDINATES: protein motif:HMM:NF012780.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HNE05205.1"
                     /translation="MNNLVRDWMSSPVVVVDPDSSVSHAMTLMRRRNIHSVVVDVSEK
                     NPSYGIVTTTDVRDKIVVAGRNPAETTVREIMSGPIVTGRAEWTLAECSRLMQERKIR
                     HLPIADEHGTLIGLISATDIFMAVEEQGWIEG"
     gene            complement(2015..>2886)
                     /locus_tag="PLT08_11845"
     CDS             complement(2015..>2886)
                     /locus_tag="PLT08_11845"
                     /inference="COORDINATES: protein motif:HMM:NF027232.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE05206.1"
                     /translation="YLVNTDFLDENTAWVQFPEMNKYPNGGTLYHTTNGGLTWESFST
                     PFSGGYIQFVDANNGWMLSDLGVGAGSMAVSVFQTSDAGKTWNRTYTNDPNLADASDT
                     LPLGGIKNLILPLNAKTAWIGGVVYAPGETYLFRSDDNGKTWVNINLILPQEAAGSEL
                     SVIGMVFLSPKTGLLALRVATETPKTIVYVTQDGGNTWTQSEVSFEGYGLLETPSANE
                     MVFYVNDQFYVTKDAGVTVQEIIPDVKFGDAIMDMSFANAQTGWIVASDASGNYTLYK
                     TTDSGSTWTVLSQ"
BASE COUNT          782 a          725 c          660 g          719 t
ORIGIN      
        1 tggaacggct cccgcgtgaa atgatgtccg cgcaggatgg ttcgctgtcc tttttgttcg
       61 gttggctgct ttttattttg catatacttc ttgcttagaa aaggaaactt tatcatgtcg
      121 tacgctcacc ccgaatacct tgtagaaaca gactgggtcg ctgaacgtct aaatgacccc
      181 aacattcgta ttatcgaatc ggacgaagat cccttgctgt atgccatagg tcacattccc
      241 ggcgccgccc aagtggactg gttcagcacg cttcaacacc cactgcgccg cgactttctc
      301 accaaagaac aatttgaaca ggttgcatcc aagcttggca ttaccaatga tacaactgtc
      361 atcttttatg gcgataagtc gaattggttt gcctgctatg cgctctggct cttccaatat
      421 tatggtcacc agaacgtgaa gatcgtaaac ggcggacgtg cgaaatggga aaaagaaggt
      481 cgcccactcg tgaaggaagt tcccacattc actgcaacta cctacaaagc caaagaagcc
      541 gataagtcca ttcgcgcctt ccgcgacgat gtcttcaagc aggttgattc aaagaaaccc
      601 ttggtagacg tccgctcgcc gaaggaatat acgggcgaat tattgcacat gcccaactac
      661 ccacaagaag gcgccacccg cggcggacac atccccggag cggtcagcat tccgtgggca
      721 caggctgtca acgaagccga ttccaccttt aagaccgccg aagaattgaa gtccctttac
      781 gaaggcaaaa acatcaaatc cgatggtgag atcatcgcct attgtcgtat tggggaacgc
      841 tcttcgttga cttggttcgt attaaagtat ttgctgggtt atcccacggt caagaactac
      901 gacggttcct ggactgaatg gggcaacctc gtagatgcac caattgaaaa ataatagtat
      961 tgtagaggcg aggtttatat cacagagtgg tataacctcg cccttacccg gaacattatg
     1021 accctaccca ataaacttca agaaattgta gatgactttg ccagcatgag ccgcgaagaa
     1081 aagctggaga cgctcatcgc atacgccgaa tccatgccag agttgccaga gcgcttccat
     1141 gcagaacgcg aaaaaatgga agcggtgcca gaatgcatga cgcccgtttt cattgccgcc
     1201 gagaaagaca gcaacaacgg catcgtcttc cacttggata ttccgaaaca atctcccacc
     1261 gtgcgcggac tggcatcgat tctgacaaat ggcttaaatg gctgctcgct tgcagagatt
     1321 atttctgtgc ctaccgagtt ttacctgcct atgaatctac aagaagccgt atcccaacag
     1381 cgcattaatg gtttcatggg tgtactggca catatgaaac aagttgctgt gcgggttggg
     1441 tcagggtaaa ttttacaagt aacaaaaaac agtcccttta cgggactgtt tttattttct
     1501 agccttcgat ccaaccttgt tcttccactg ccataaaaat atcggtggca gagatcaatc
     1561 cgatcaaggt gccatgttca tctgcaattg gcaagtggcg aatttttctt tcttgcatca
     1621 gtcgagagca ttctgccagc gtccattcgg cacgcccagt aacaatggga ccagacataa
     1681 tctcgcgcac agtggtctct gctgggttac gcccggcaac cacgatctta tcgcgcacat
     1741 ctgttgttgt tacaatgcca tatgaagggt tcttctcgct aacatccaca accacgctat
     1801 gaatattgcg acgacgcatg agcgtcatgg catgtgaaac gctcgaatca gggtctacaa
     1861 caacaaccgg gctggacatc caatcacgaa ctaagttatt cataattctc tctcctttat
     1921 agatatttta cagtatgggt gtaactatca ggttactcac agcgtaacca aagggtcgag
     1981 caatagtatc aagcagcggc ggggaaagaa gcgtttactg acttagaacc gtccatgttg
     2041 agccgctatc cgttgtctta tacaaagtat agtttccaga agcatcagaa gcaacgatcc
     2101 aaccagtttg agcattcgca aaactcatat ccatgatggc gtcgccgaac ttcacatcgg
     2161 gaataatttc ttggacagta acacccgcat ctttcgtgac gtagaactgg tcattgacat
     2221 aaaagaccat ttcattcgct gacggcgttt ccaataatcc ataaccttca aacgaaactt
     2281 cagattgcgt ccacgtgtta ccgccatctt gggtgacgta tacaatggtc ttgggtgttt
     2341 ctgtggcaac ccgcaacgca agcagacctg tcttgggcga gagaaagacc attccgatca
     2401 cgctcagttc actgcctgct gcttcttgcg gaaggataag gttgatattg acccaggttt
     2461 tgccattatc gtcactgcgg aaaagatagg tttcaccggg ggcatagacc acgccaccga
     2521 tccaagctgt ttttgcattg agcggcaaga tgagattctt aataccaccc aacggaagcg
     2581 tatcacttgc atcagcaaga ttggggtcat tggtgtaagt gcgattccaa gttttgccag
     2641 catcactggt ctggaaaaca gaaaccgcca tagaaccagc accaacacca aggtcagata
     2701 gcatccatcc gttgtttgca tcaacaaatt gaatataacc gccgctgaat ggcgtagaaa
     2761 agctttccca tgttaagccg ccattggtgg tgtgatagag cgtaccacca ttggggtatt
     2821 tgttcatttc agggaattgc acccaagccg tattctcatc cagaaaatcg gtgttgacga
     2881 gatagc
//
LOCUS       DAOQJE010000462         2882 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_23681_length_2882_cov_7.608773, whole genome shotgun sequence.
ACCESSION   DAOQJE010000462 DAOQJE010000000
VERSION     DAOQJE010000462.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2882)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2882)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2882
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23681_length_2882_cov_7.608773"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1340)
                     /locus_tag="PLT08_11850"
     CDS             complement(<1..1340)
                     /locus_tag="PLT08_11850"
                     /inference="COORDINATES: protein motif:HMM:NF012770.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HNE05207.1"
                     /translation="MKKVFLFVSLLVITSLSCKALSPKPTEQTTPPTLSPASGDTQSD
                     TAAKLQELGGVTCEDAPDLTCVTIQVPLNHYDSANTETIDVVFGVAPATGERYGMFVQ
                     AFPGGPGGEGISSSYMGYFDPTVLEHYDMVYYDQRGLGLSSPLSCPKAYAADFVEYLT
                     TPDTAGLEGLDTPEEQKTAIDDSRAYAQNCIAEMGIDPAKLAFYGTDQVAEDIDSFRA
                     LLGDEKLWIYGVSYGTAVAQTYATEHPDRVAGMILDGTINLTLDGVQGSLAQEKAFDK
                     VLVAVLNACDEDEVCHADMGGENALAVYDKFAAQVSKDPIEYEYPLPNGEKVKGVFTF
                     NALEFTTAYQMYSLTTRMIFLRALAAANQGDIIPMLRLMYENATIDPATFEYIGDPTF
                     SDTMFLSVLCTDDTFFTGTQEERIQQSIEAGQASNGTVPRLDGSVYTGIDCAFWP"
     gene            complement(1360..2367)
                     /locus_tag="PLT08_11855"
     CDS             complement(1360..2367)
                     /locus_tag="PLT08_11855"
                     /inference="COORDINATES: protein motif:HMM:NF013046.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histone deacetylase"
                     /protein_id="HNE05208.1"
                     /translation="MSTVFTFVPSREHEYPDHPERPGRLDILEPLLPSFGAEQIQAIR
                     ATEADVALVHPVKMIRSLQEACMEGPGVIDYAPTYVTQTSYEDALQAVGGVLTCSHAV
                     MNGDARNAFAIVRPPGHHAEPDRAMGFCLFSNIAIAAQDLLRKGMKRVMVIDYDAHHG
                     NGTQAAFLHEERLGFVSTHQWGIYPGTGWIDDAPHANQRIVNVPLPAYAGDKTFVRIA
                     DEVFAPMVRAFKPEMLLISAGFDSHWNDPITSLGLSTRGFYEVSKKLVEMAEEHCGGK
                     IVFVLEGGYDPRNVANGVGAVFDALTSRPLGNEAGDASPYKEPDFESRLKEIRQKHDL
                     I"
     gene            complement(2470..>2882)
                     /locus_tag="PLT08_11860"
     CDS             complement(2470..>2882)
                     /locus_tag="PLT08_11860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE05209.1"
                     /translation="KPAYYDHNLGQKMEFPAPGESSQLAQSLISNYGYLYYPLGVESD
                     GNCVEVLESTFNTKIILEASSIIMDGKSTGPYTWTIDYTYLLNTTNNPVPNATFIVNS
                     AIESTDALPTVPVISDAFWEEYAKFIWGKLTPKH"
BASE COUNT          728 a          703 c          712 g          739 t
ORIGIN      
        1 ggccagaagg cacagtcgat gccggtgtag accgagccgt ccaaacgtgg aacggttccg
       61 ttcgacgcct gacccgcttc aatcgattgt tgaatgcgtt cctcttgggt gccggtgaag
      121 aaggtatcgt cagtacatag cacgctgagg aacatcgtat cggagaaagt tgggtcgccg
      181 atgtattcaa aggttgccgg gtctatggtc gcattttcgt acatcaagcg caacatgggg
      241 atgatgtcgc cttggtttgc ggcagccaat gctcttaaga aaatcatacg ggttgtaaga
      301 gagtacatct gatacgccgt ggtgaattca agagcattga aggtgaacac accttttact
      361 ttttcaccgt ttggtaacgg atattcatat tcaatcgggt cttttgagac ttgagctgca
      421 aacttatcgt atacagccag cgcattctcg ccgcccatat ctgcatgaca aacttcgtct
      481 tcgtcacagg cattcaagac cgctaccaat actttgtcga aggctttctc ttgtgcaagc
      541 gaaccctgca ctccatccaa tgtcaagtta atggtgccat ccagaatcat gcctgccaca
      601 cggtcagggt gttcggtggc ataggtttga gcgactgccg tgccatagct tacgccatag
      661 atccaaagct tttcatcacc caacaaagca cggaatgaat caatatcttc cgctacctgg
      721 tcggtgccat aaaaagctaa cttggcaggg tcaatgccca tttctgcgat acagttttga
      781 gcataggcac gcgaatcatc aatggcggtt ttctgttctt cgggtgtatc caaaccttcc
      841 aacccggcag tatcgggcgt ggtgaggtac tcaacaaaat ctgcggcata tgctttcggg
      901 catgagagcg gactagaaag cccaagccca cgctggtcat aatagaccat atcgtaatgt
      961 tcaagaaccg ttgggtcgaa ataacccata tacgatgaac taatgccttc accgccaggt
     1021 ccgcctggga aggcttgtac gaacatccca tagcgttcac ctgtggcagg tgccacacca
     1081 aagaccacgt caatggtttc tgtgttggca gaatcataat gattgagtgg aacctgaatg
     1141 gtcacacacg tgaggtcagg tgcatcttca caagtcacac cgcctaattc ctgaagcttg
     1201 gcggcagtat cactttgagt atctccactt gcagggctga gggtgggagg agtggtctgt
     1261 tctgtgggct tcggtgataa agctttacaa cttaaagacg tgattacgag taacgatacg
     1321 aacaaaaaaa ctttcttcat ataaatttcc ttttgatgtt tagataaggt cgtgcttttg
     1381 gcggatctct ttcaagcgtg actcgaaatc cggctcttta tagggagatg catcgcccgc
     1441 ctcattccct aagggacggc tggtcagcgc atcgaaaact gcgcctacgc cgttcgcgac
     1501 gtttcgcgga tcatacccac cttccaatac aaaaacgatt tttcccccgc aatgttcttc
     1561 tgccatttcc accaattttt tcgacacttc ataaaagcca cgtgtcgaca atcccaatga
     1621 agtgatgggg tcattccaat gcgagtcaaa ccctgcggag atcaataaca tctcgggttt
     1681 gaaggcacgc accatcggtg caaagacttc gtctgcgatg cgcacaaagg tcttgtcacc
     1741 tgcataagcc ggcaacggca cattgacaat gcgttggttg gcgtgaggcg catcgtctat
     1801 ccagccggtg ccggggtaaa ttccccattg atgcgtggag acaaatccca gccgttcttc
     1861 gtgcaaaaat gctgcttgtg tcccattgcc atgatgtgcg tcataatcga tcaccatcac
     1921 gcgcttcatg ccttttctca gcaaatcctg tgcagcgatg gcgatattgc tgaagagaca
     1981 aaaccccatc gcgcggtctg gttcggcgtg gtgccccggc gggcggacga tggcaaatgc
     2041 attacgagca tctccattca tcaccgcatg agaacaggtc aacacgccac cgacggcttg
     2101 caacgcatct tcataagaag tctgcgttac ataggtaggc gcataatcaa tgacaccggg
     2161 tccttccatg caggcttctt gcagagaccg aatcatcttc accgggtgga cgagagccac
     2221 atccgcttcg gttgcccgaa tcgcttggat ctgttccgcg ccgaaagagg gtagcaaagg
     2281 ctccaaaata tccaacctgc ctgggcgttc ggggtggtct ggatattcat gttcccgcga
     2341 tgggacaaat gtaaatacgg tagacatgat ttaattatag ccccctgttg gtgaattcat
     2401 gagcttaagc tacagaagtc acagcggatt tgagcatttt ttaaaacctg ctgtggaaaa
     2461 aagtcataat tagtgttttg gcgtgagctt gccccaaata aattttgcgt attcttccca
     2521 gaacgcatct gagatgacgg gaacagtggg caaggcatcc gtcgactcaa tcgcagaatt
     2581 aacgataaag gttgcattgg ggacggggtt attcgttgtg tttaagagat aggtgtaatc
     2641 aatcgtccat gtatatggtc ccgtagactt tccatccata ataatggagg atgcttcaag
     2701 gatgatcttg gtgttgaagg tcgattcaag gacttccaca caattgccat cggattctac
     2761 gcctaatgga tagtacaaat atccatagtt tgatattaaa gattgtgcga gctgagaaga
     2821 ttcccccggc gcggggaatt ccattttttg cccaaggtta tggtcatagt atgcaggttt
     2881 aa
//
LOCUS       DAOQJE010000463         2879 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_23716_length_2879_cov_8.072238, whole genome shotgun sequence.
ACCESSION   DAOQJE010000463 DAOQJE010000000
VERSION     DAOQJE010000463.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2879)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2879)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2879
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23716_length_2879_cov_8.072238"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..704
                     /locus_tag="PLT08_11865"
     CDS             <1..704
                     /locus_tag="PLT08_11865"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HNE05210.1"
                     /translation="LSIMPSTHLIENYNILLGKGLNLPRGFANSTFLAVSSTVVSVYF
                     SMLTAYGIVVYDFKGKHAFSNFIVVLVMIPMQLSIIGFYQYMSKLGLTDNYASLILPL
                     IANAGGVFFGKQYLESMIIQDLIDAARIDGASEMAIFHQVMMPLAIPGAVTMGIFAFI
                     TSWNNFFNAFILISSIEKYTLPMLVQTLRGDVYRTEFGAIYLGLAATIVPIILLYIFF
                     SRYVVSGIAIGAVKE"
     gene            871..2037
                     /gene="xylA"
                     /locus_tag="PLT08_11870"
     CDS             871..2037
                     /gene="xylA"
                     /locus_tag="PLT08_11870"
                     /EC_number="5.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009045 - xylose isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0042732 - D-xylose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xylose isomerase"
                     /protein_id="HNE05211.1"
                     /translation="MSKYSPSPENKFTFGLWTVGNVGRDPFGSPVRDGKSPADLVRLL
                     GEVGAYGVNFHDNDLIPIDATPSERDAILKEFKKALADTGIIVPMATTNLFSEPIFKD
                     GAFTSNDSKVRAYALQKTMNAIDLGVELGAKTYVFWGGREGTESDATKDPITSIKRSR
                     EAMNFLCEYVLDKKYDLKFALEAKPNEPRGDIYNSTTGHMLAFIETLDHPDMVGVNPE
                     VAHEHMAGLNFVHGVAQALEAGKLFHIDLNDQAFGRYDQDFRFGAVNIKSAFFLVKLL
                     EDNKYNNPRHFDAHAYRTEDYEGVKDFARGCMHTYLVLKEKAAQWNADKEIQALVAEI
                     NADDGSMAQYFGKYSSAKASALKAQAFDRNAIASRGLKYERLDQLTIDVLLGVR"
     gene            2053..>2879
                     /locus_tag="PLT08_11875"
     CDS             2053..>2879
                     /locus_tag="PLT08_11875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 N-terminal
                     domain-containing protein"
                     /protein_id="HNE05212.1"
                     /translation="MSNSNIAEKPIYLDTSRPREERVKDLISHLTLEEKVGLMSHPTN
                     GIPRLNIPAYNYWNEALHGVARNGRATVFPQAIAMAATWDKDLIHKIGNAISDEGRAK
                     YHAALKRNGYTQQYQGITFWSPNVNIFRDPRWGRGQETWGEDPFFTGEMAAEFVKGLQ
                     GNDPTYLKTAACAKHFAVHSGPEKDRHSFNAIVTKRELYDTYLPAFKKLVTEAKVEAV
                     MGAYNRTLDEVCCASKLLIEDILRGEWGFDGHFVSDCMALSDFHLNHKVTKDAAESAA
                     "
BASE COUNT          750 a          756 c          647 g          726 t
ORIGIN      
        1 gtctcagcat tatgcccagt acgcatctta ttgaaaatta caacatcctc cttggaaaag
       61 ggttgaacct tcccagaggg tttgcaaata gcaccttttt agcggtatcg tccacagtgg
      121 tgtctgtcta tttttccatg ttgactgctt atggcattgt ggtctatgat ttcaaaggca
      181 aacatgcctt ctcaaacttc attgtcgtgt tggtcatgat tcccatgcag ctctcgatca
      241 tcggttttta tcagtacatg tcgaaactgg ggctaacaga taattacgca tctctgatcc
      301 tgccgctgat agccaatgca ggcggggtgt tcttcggaaa acaatattta gaatccatga
      361 tcattcaaga cttgatcgat gctgctcgaa tcgacggcgc cagtgaaatg gcaatattcc
      421 atcaagtgat gatgccgctt gccattcccg gtgcggtgac catgggtatc tttgccttca
      481 ttacatcctg gaataacttc ttcaacgcct tcatcctgat ctcttcgatc gaaaaataca
      541 cacttcccat gttggtgcaa accctgcgcg gagatgtgta tcgaaccgaa tttggcgcca
      601 tttaccttgg cttggctgcc accattgttc cgatcattct tctttatatt ttcttctcac
      661 gatacgtggt tagcggcatt gccattggag ctgtaaagga ataaagcgcc tcatacttta
      721 acttttcttg ttctaccctc ttacacttta ccctccatct tagttttgct tcattcattt
      781 gttctatctc cttatgcgtt ccccgcctcc atgtggaggc ggggctggga gagtattgct
      841 aaaaatcagt aaaaaccaag gagtaatcta atgtccaagt attccccatc ccctgaaaac
      901 aagttcacct tcggtctgtg gaccgttggc aatgttggac gcgacccgtt cggtagcccg
      961 gttcgcgatg gcaaatctcc cgcagacttg gttcgcctgc tcggagaagt tggcgcatac
     1021 ggtgtcaact ttcatgataa cgacctgatt cccattgatg ctacgccctc tgaacgagat
     1081 gccattctga aggaattcaa gaaggcgctt gccgatacag gcatcatcgt gccgatggca
     1141 accaccaatt tgttctctga acccattttc aaagatggtg catttacatc aaacgactca
     1201 aaagtccgcg catatgcgtt gcagaagacc atgaacgcca tcgatctcgg cgtggaactc
     1261 ggtgccaaaa cctatgtatt ctggggcggt cgcgaaggga cagagagtga cgccaccaaa
     1321 gaccccatca cctccatcaa acgctcgcgc gaagcgatga acttcctctg cgaatatgta
     1381 ttagacaaaa aatatgacct caagtttgcg ctcgaagcca aacccaatga accgcgcggc
     1441 gacatttaca actccaccac gggtcatatg ttggcattca ttgaaaccct tgatcatccc
     1501 gatatggtcg gcgtcaaccc tgaagtggca catgaacaca tggcagggct caacttcgta
     1561 cacggcgtag cccaggcgtt ggaagcgggc aaactcttcc acattgactt gaacgaccaa
     1621 gccttcggtc gctatgatca agacttccgc tttggtgcgg tcaacatcaa gagcgctttc
     1681 ttcctcgtca aattactcga agacaataaa tacaacaatc cacgtcactt tgatgctcat
     1741 gcatatcgca ccgaagacta cgaaggggtt aaagatttcg cgcgtggctg tatgcacacc
     1801 tatctcgttc ttaaagaaaa agccgcacaa tggaatgcgg ataaagagat ccaagctctc
     1861 gttgcagaga tcaatgcaga tgacggttcc atggcgcaat actttggcaa gtactcctca
     1921 gccaaagcca gcgctctcaa agctcaagcc ttcgaccgca atgccatcgc ctctcgcgga
     1981 ttgaaatacg aacgcctcga ccagttgacc atcgacgtac tacttggcgt gcgataacgt
     2041 aggagccctt gaatgagtaa ctcgaatatc gcggaaaaac ccatctatct tgatacgtcg
     2101 cgtccacggg aagaacgtgt aaaagacttg atcagccacc ttactttgga agaaaaagtg
     2161 gggctgatga gtcaccccac caatgggatt ccgcgcctga acatccctgc atacaactac
     2221 tggaatgaag cgttacatgg cgttgctcgt aatggaagag ccactgtttt tccacaagct
     2281 attgccatgg ctgccacgtg ggacaaggac ctgatccaca aaataggcaa tgccatcagt
     2341 gacgaaggtc gcgccaaata ccatgctgct ttaaaacgca atggctacac ccaacaatat
     2401 caaggcatta ccttctggtc gccaaatgtg aatatatttc gtgacccacg ttgggggcgc
     2461 ggacaagaaa cctggggcga agatcccttc tttaccggtg aaatggcggc tgaattcgtc
     2521 aaaggcttgc aaggcaatga ccctacctat ctcaaaaccg ccgcctgtgc aaaacacttt
     2581 gctgtgcatt ctggtccaga aaaagaccgc cactccttta atgccattgt aaccaaacgt
     2641 gagttatacg acacctacct gcctgctttc aaaaaacttg tcaccgaagc caaagtggaa
     2701 gctgtgatgg gtgcctacaa ccgcaccttg gacgaagtgt gttgtgccag caaacttctg
     2761 atcgaagaca tcttgcgcgg agaatggggc tttgacgggc actttgtttc agactgcatg
     2821 gcgctgtcag attttcatct caaccacaag gtcacgaaag atgcggcgga atccgctgc
//
LOCUS       DAOQJE010000464         2859 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_23998_length_2859_cov_6.424394, whole genome shotgun sequence.
ACCESSION   DAOQJE010000464 DAOQJE010000000
VERSION     DAOQJE010000464.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2859)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2859)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2859
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23998_length_2859_cov_6.424394"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1131
                     /locus_tag="PLT08_11880"
     CDS             <1..1131
                     /locus_tag="PLT08_11880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HNE05213.1"
                     /translation="VEIMRGCTRGCRFCQAGMITRPVRERTVQEVVDAADAAVRSTGF
                     EELALLSLSSSDYTYVNDLVDAISKRFEGRKLTVSLPSLRIESVSVDLMEKLKQHRSG
                     GFTLAPEAASERMRRIINKYIPDEDIINTTRDIYARGWTTIKLYFMIGHPSETIEDVQ
                     AIADLCKRVLAEGRKVAGWKVKLHAGVSTFVPKPQTPFQWVACDTRDNILEKQALLKR
                     EFFKDKSIKLSWTKPDDTLVEAWLSRGDRRMSEVIYTAWKNGAKFDAWEEGRKFETWM
                     AAFEENGIDHAFYTHRQRRTDEIFPWEHITAAVRKNFLFQDFRMSLEGQIRVDCRLNC
                     FACGILPTFANLRRENPGEGWKCPEVKTPARKVELELPIAGD"
     gene            1140..1667
                     /locus_tag="PLT08_11885"
     CDS             1140..1667
                     /locus_tag="PLT08_11885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE05214.1"
                     /translation="MNKAILLERLQNERDQFELLLNRLGFARQLTMRGVVAGLTVKDL
                     VAEVFSHEQFIADRLSEILHGDTYAPSASFTALENFQQEYGYPDYESSLIEKDKHDLS
                     VVDAYKHIEFDEIISQELAVYGNILESIEKLTHHEFLDHDLYNRIAEHTYRPYRRTSS
                     IINRWLKRIASETKK"
     gene            1670..2299
                     /locus_tag="PLT08_11890"
     CDS             1670..2299
                     /locus_tag="PLT08_11890"
                     /inference="COORDINATES: protein motif:HMM:TIGR03936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03936 family radical SAM-associated protein"
                     /protein_id="HNE05215.1"
                     /translation="MRVRITFTKQGALRYIGHLDLHRVWERALRRASLPISYSQGFHP
                     QPKISLAAALPLGFSSRAEVIDVRLNEEIPTEEIHTRLKDNLPPDIKVTDVKSVDERL
                     PALQTLVLSAAYDVHLTEPVAGSELTRRVESLMQAESIIRERRGKTYDLRPLVEMLSV
                     ITQADGTVWLKMTLAAREGATGRPEEVLTVLEIEPETARVERTRLIFKE"
     gene            2435..>2859
                     /locus_tag="PLT08_11895"
     CDS             2435..>2859
                     /locus_tag="PLT08_11895"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070007 - glutamic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PrsW family glutamic-type intramembrane
                     protease"
                     /protein_id="HNE05216.1"
                     /translation="MALLAAIPMAFIPAFFFSWFLYWLDRYEKEPRWLLLMTFFWGGF
                     VAIIGTLIVASIFEVGFSFVLNDAVLEDIAGGSITAPLVEEFMKGFAVMLVFFMFRKE
                     FDSILDGIIYGGIAGLGFAATENVLYFLGQYNEAGWGGM"
BASE COUNT          741 a          694 c          671 g          753 t
ORIGIN      
        1 gtcgaaatta tgcgtgggtg cacccgcggc tgtcgcttct gccaggcagg gatgatcact
       61 cgcccggtcc gcgaacgaac cgtacaagaa gtggtagatg ccgctgatgc tgcagtccgc
      121 tctaccgggt ttgaagagct agcgctgctg tcgctatcct catcagacta cacttatgtc
      181 aacgaccttg tggatgccat cagcaaacgg tttgaaggtc gaaaattgac tgtctcgctg
      241 ccttctctgc gcatcgagtc tgtttcggta gatttaatgg aaaagctcaa acagcatcgc
      301 tcaggcgggt ttactcttgc tcctgaagct gccagtgaac gcatgcgccg tatcatcaat
      361 aagtacattc ctgacgaaga tatcatcaat accactcgcg atatttacgc ccgcggttgg
      421 acgactatca aactctactt catgatcggt caccccagtg aaacgattga agatgtacaa
      481 gccattgcag atctgtgcaa acgcgtcctt gctgaaggac gtaaagtggc gggttggaaa
      541 gtgaagttac atgctggtgt cagcactttt gtgcctaaac cacaaacccc tttccaatgg
      601 gttgcctgtg acacacgcga taatattctc gaaaagcaag ccttgctcaa acgtgaattc
      661 ttcaaagata aaagtatcaa actaagctgg acaaagcctg acgacactct tgttgaagcc
      721 tggctttctc gtggcgaccg ccgcatgtct gaggtgatct acacagcatg gaaaaacggt
      781 gccaaattcg atgcctggga agaagggcgc aaattcgaaa cgtggatggc tgccttcgaa
      841 gaaaatggca ttgaccatgc cttctatacc catcgtcaac gccgtacgga tgaaatcttc
      901 ccatgggaac atatcacagc cgctgttcgt aagaacttcc tcttccaaga ttttcgtatg
      961 tcgctcgaag gtcagatccg cgtggactgt cgcttgaatt gtttcgcttg cggaattctg
     1021 cccacctttg cgaacctgcg ccgcgaaaac cccggtgaag gttggaaatg ccccgaagtg
     1081 aaaacccctg cccgtaaagt agaattggag cttcccatcg ctggggatta gaaacaagca
     1141 tgaacaaagc catactcctc gaacgcttgc aaaatgaacg cgaccagttt gaacttttac
     1201 tgaatcgcct tggttttgcg cgtcaactaa ccatgcgcgg agtggtagct gggcttactg
     1261 tcaaagatct tgtagcagaa gttttttcac atgagcaatt cattgcagac cgtttaagcg
     1321 aaattctgca tggtgatacc tatgctccat cagcttcttt cacagccctc gaaaattttc
     1381 agcaagaata tggttatccg gattacgagt cttcgttgat cgaaaaagat aagcatgacc
     1441 tatctgtggt agatgcttat aaacatatcg aattcgatga aatcatttct caagaactgg
     1501 cagtatatgg aaatatccta gagtcaattg aaaaactgac acatcatgaa tttctcgacc
     1561 acgacctgta caatcgcatc gcggaacaca cttatcgccc ctaccgccgt accagttcca
     1621 tcatcaaccg ctggctaaaa cgcatcgcct cagagacaaa aaaataaata tgcgagttcg
     1681 aattacattc accaaacaag gcgcattgcg ctatattggt catcttgacc tgcaccgagt
     1741 atgggaacgg gcattacgcc gtgccagtct gcccatttca tactcccaag gctttcaccc
     1801 ccaacccaag atcagcctag ctgctgcatt gccgcttggg ttttcctccc gtgctgaagt
     1861 gatagacgtg cggttaaacg aagaaatccc aaccgaagaa atccatactc ggttgaagga
     1921 taatcttccg cccgatatta aagtgacaga tgtgaagtca gtggatgaac gtttgcccgc
     1981 tttgcaaaca ctggtgttat cagcggcgta tgatgtccat ttgacggaac cagttgccgg
     2041 gtccgaactg acgcgtaggg ttgagtccct catgcaggct gaatctatta tccgagaacg
     2101 tcgtggcaaa acctatgatc tgcgtccgtt ggttgaaatg ctaagtgtga tcacgcaggc
     2161 agatggcacc gtttggttga aaatgactct agctgctcgc gaaggcgcaa ccgggcgccc
     2221 agaagaagta ttgactgtgc ttgagattga accggaaacg gcgcgggtag agcgtactcg
     2281 cctaattttc aaagaataaa caaaacgtct ggcttagcca gacgtttgat tttagataag
     2341 cgttttgaaa taccccctta agttctattg atttttagcc cagcatcagt aaaattaatc
     2401 agatatctaa tttcaactca ctggaggaat ttttatggca ctactagctg caattccaat
     2461 ggcattcatc ccagcattct ttttctcgtg gttcttatat tggctcgacc gttacgaaaa
     2521 agaaccgcgc tggctgctcc taatgacctt tttctggggt ggctttgttg ccattatcgg
     2581 cacattgatc gttgcttcga tttttgaggt cggtttcagc tttgtgctga acgacgctgt
     2641 ccttgaagat attgctggcg gctccattac tgctccatta gtcgaagaat tcatgaaggg
     2701 ctttgccgta atgctggtct tcttcatgtt ccgcaaagag tttgactcca tcctcgacgg
     2761 tattatttat ggtggcattg cagggctcgg cttcgcagca actgaaaatg ttttgtattt
     2821 tctaggtcaa tataatgaag cgggctgggg cggcatgtt
//
LOCUS       DAOQJE010000465         2857 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_24021_length_2857_cov_5.922912, whole genome shotgun sequence.
ACCESSION   DAOQJE010000465 DAOQJE010000000
VERSION     DAOQJE010000465.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2857)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2857)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2857
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24021_length_2857_cov_5.922912"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1227
                     /locus_tag="PLT08_11900"
     CDS             <1..1227
                     /locus_tag="PLT08_11900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE05217.1"
                     /translation="SQAQNIGAQSPAEPAKTQWSENAANIGEEFFAEFEKGSSQSSTD
                     ETGMWLRDLDNENAQPSSSASDDLPDWMKPEEEQPLERGSIPEWLDESAHEKKQALDT
                     SSLPSWLEDLDQPEKSISEKSLVQNQDLSDWLSGLDDEPGLNIDPETLRAPQPPVETK
                     RPVAEVQPQATSEDLPDWMAGEDEVEDAPEDWAPPAQPTVAPVSLSADLPDWLQGLDE
                     DGQADDNLSGDDTPPWIHREQYETEGSVPQPPKPTSPSDWQPVSAAQKPKEETNQPAA
                     QPRSGQSQQQPQRLVQNPPTPEPVASEKKKSPVKQQQGLPRQKKSEAQGTVNMLGLAK
                     TELDRGDIPEALMHYGKLIKKGKHLEEVIGNLTESLYRYPVEVGIWQALGDAYMRANR
                     LKEALEAYNKAEELIR"
     gene            1303..1755
                     /gene="ndk"
                     /locus_tag="PLT08_11905"
     CDS             1303..1755
                     /gene="ndk"
                     /locus_tag="PLT08_11905"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004550 - nucleoside diphosphate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0006165 - nucleoside diphosphate
                     phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HNE05218.1"
                     /translation="MEKSLVLVKPDGVQRGLIGEAISRFERRGLRLIGAKFIWVSKEL
                     AEAHYAEHKGKGFYDGLISYITSAPVMAMVWEGPNAVAAIRQTVGSTKPWEAAPGTIR
                     HDFAIEVGRNLIHASDKPETGDRETALWFKKEELVEWSRAIDAWVFEK"
     gene            complement(1800..2129)
                     /gene="rsfS"
                     /locus_tag="PLT08_11910"
     CDS             complement(1800..2129)
                     /gene="rsfS"
                     /locus_tag="PLT08_11910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HNE05219.1"
                     /translation="MARDIVDALEDKKGEDIVLMDIKEIASFTDYFVICTGTSDRMLD
                     ALANSAIDEVRKKHKKKAKKQGNSRDGWVVVDFGDVVLHLLSPDQREYYQIEDLWEAG
                     KILLRLK"
     gene            complement(2148..2726)
                     /gene="thpR"
                     /locus_tag="PLT08_11915"
     CDS             complement(2148..2726)
                     /gene="thpR"
                     /locus_tag="PLT08_11915"
                     /inference="COORDINATES: protein motif:HMM:TIGR02258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008664 - 2'-5'-RNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2',3'-cyclic phosphodiesterase"
                     /protein_id="HNE05220.1"
                     /translation="MNQIRTFIALDFPLSILESIEQQTQRLRQTLGGDFIRWVPTHNL
                     HLTLKFLGNIPISHLEFIKRMLTQAVDSSSHFDLQISGFGSFPNSKRPRVLWVGVHTP
                     ATLATLQKAVEEGAHHLGYEKEERPFSPHLTLGRVRQGINVTELQKISNTISSFQLGK
                     IGTARVDSVHLYQSDLNSEGSVYTKLFSTPLR"
BASE COUNT          762 a          630 c          743 g          722 t
ORIGIN      
        1 tcacaggctc aaaatattgg agcacagtct cctgctgaac ctgccaaaac ccagtggagc
       61 gagaatgctg cgaatattgg ggaagaattc tttgcagaat ttgaaaaagg ctcttctcaa
      121 tcttctactg atgaaaccgg aatgtggtta cgagacctcg ataacgaaaa tgcacagccc
      181 agttcatcag caagtgatga tcttccagat tggatgaagc ctgaagaaga acaacccctc
      241 gaacgtggtt ctatccctga atggttggat gaatccgcgc atgagaagaa acaggcatta
      301 gatacgtcta gtttgcctag ctggttggaa gatcttgacc agcctgaaaa gtctatatcc
      361 gaaaagtccc ttgttcagaa ccaagacctc tctgattggt taagcgggct tgatgacgag
      421 cctggtctaa atattgatcc cgaaaccttg cgcgcaccgc aaccgccagt tgaaaccaaa
      481 cgtccagtag cggaggttca gcctcaggcg acctctgaag acttgccaga ttggatggct
      541 ggtgaagatg aggtagagga tgctcctgaa gactgggcgc ctcccgctca gccgacagtt
      601 gctcctgttt ctctaagcgc agatttgccc gattggcttc aaggtttgga tgaagatgga
      661 caggctgacg ataatttgtc tggtgacgat acgccgccat ggattcaccg tgagcaatac
      721 gaaacagaag gtagcgtgcc gcaaccaccg aagccgactt cgccatctga ttggcaaccg
      781 gtttcagcgg cgcaaaagcc taaggaagaa acaaatcagc cagcggcgca gccccgaagc
      841 ggacagtctc agcaacagcc acaacgtctg gttcaaaatc cgccaacgcc cgaaccggtt
      901 gcgtctgaaa agaagaaatc tcctgtgaag caacaacaag ggttgccgcg tcagaaaaag
      961 tctgaggcgc agggtacggt caatatgttg ggacttgcaa agactgaact ggaccgtggt
     1021 gacatccctg aggcgctgat gcattatggg aagttgatca agaagggcaa acaccttgaa
     1081 gaagtgattg gtaacctgac tgagtctttg tatcgatacc cggttgaagt aggtatctgg
     1141 caggctctag gtgatgccta catgcgggca aatcgcttga aagaagcgct cgaagcctat
     1201 aacaaagcgg aagagctcat acggtaacat tgcgaacgcc gctggaaaca gcggcgtttg
     1261 atagcactag cttggcaatg aaataatttt tggaggaact ctgtggaaaa atctctcgta
     1321 ctcgtgaaac cggatggtgt tcagcgcggt ttaatcggtg aagcaatctc tcgcttcgaa
     1381 cgccgcggtc tgcgcctgat cggtgctaaa tttatttggg ttagcaagga attggcagaa
     1441 gctcattatg ccgaacataa aggtaaggga ttttatgatg ggctgatttc ctatatcacc
     1501 tctgcgccgg tgatggcgat ggtgtgggaa ggtcctaacg ctgtagctgc tattcgtcag
     1561 actgtgggct caaccaagcc ttgggaagcc gcgcctggca ccattcgcca cgatttcgct
     1621 attgaagtag gacgcaatct tattcacgct tcagataagc cggaaactgg cgaccgtgaa
     1681 actgctctct ggttcaagaa ggaagaactg gttgagtggt cgcgcgctat tgatgcttgg
     1741 gtatttgaaa agtaaaaaat aagtaataaa aaagcccttc cataggaagg gcttttttat
     1801 tactttaatc tcaacagaat tttcccggct tcccataagt cttcgatctg gtagtattcg
     1861 cgttggtctg gagaaagaag gtgcaagacc acatctccaa agtcaacgac cacccagcca
     1921 tcacgggaat tgccttgttt tttggctttt ttcttatgtt ttttacggac ttcatcaatg
     1981 gcgctgttag caagcgcatc taacatgcgg tcactggtgc cggtacagat gacgaaataa
     2041 tcagtaaaag aagcaatttc tttgatgtcc attaggacga tgtcttcgcc ttttttatct
     2101 tcaagagcat caacaatatc acgagccata tctagggtat tcagagttca cctcaatggg
     2161 gttgaaaata acttcgtata gaccgaacct tctgagttca agtcactttg ataaagatgc
     2221 acagagtcta ctctggctgt gccaattttg ccaagttgaa atgaggaaat cgtattactg
     2281 atcttctgta attctgtgac gttaattcct tgacggacac gccctaatgt aaggtgcggc
     2341 gagaaggggc gttcttcttt ttcataacca aggtgatgag cgccttcttc gacggctttt
     2401 tggagtgtgg caagagtggc tggtgtgtgg actccgaccc agaggacgcg aggtcgcttt
     2461 gagtttggga aagagccgaa tccgcttatt tgtaaatcga aatgcgaaga tgaatcaact
     2521 gcttgggtga gcatccgttt gatgaattcc agatgcgaga tggggatgtt gcctaggaat
     2581 tttaacgtca aatgcagatt atgagttgga acccaacgga tgaaatcccc ccctaaggtt
     2641 tggcgcaggc gctgtgtttg ctgttctatt gattcgagaa tggataatgg aaaatccaga
     2701 gcaataaagg ttcggatctg attcattcct agcttgaggc ttgcagtacc ttctctacag
     2761 catttttgag gtcttgaaag ccaacgggtt tggtgaggta cattgaagcc ccagcttcaa
     2821 gcccggtttt gatatcgccg ggcatgcttt tggctga
//
LOCUS       DAOQJE010000466         2852 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_24085_length_2852_cov_8.526636, whole genome shotgun sequence.
ACCESSION   DAOQJE010000466 DAOQJE010000000
VERSION     DAOQJE010000466.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2852)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2852)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2852
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24085_length_2852_cov_8.526636"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            149..1612
                     /locus_tag="PLT08_11920"
     CDS             149..1612
                     /locus_tag="PLT08_11920"
                     /inference="COORDINATES: protein motif:HMM:NF024808.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HNE05221.1"
                     /translation="MKRLSYILSLLVVVSMFLAACAPAATEEAPAATEAAAEATDAPA
                     ATEAAPAELSGTLNVWSFTNEIRTMAIAFEGVHPNVDVVYTMIPMTNGEYQTKVKAAA
                     GTADSPDVVALEAAFVKEWVESDLLADQSDLLPLAEELKTFPSVVEVGTYDGITKAYS
                     YQATPGAFFYRRSIAKECLGTDDPDAVQAMVADLDKFVETAAKIKECGTGDYFTVGTS
                     GEMFNPFLANREQPWVVDGELVIDPMVLEYVKFAKLMRENGYESQATQWSEGWFAGMN
                     DTLKDANGTPKKVFSYFLPTWGLPYVLIPNSKSDTTDTGGDWAMINGPMAYQWGGTWV
                     GALEGSPNAELAKEFIKFVALNEDNLTNWATGVYTNEYLKAIDPETPDDQAQAPGDFV
                     SSQVVVEKITSTFDGSDLAKWLGGQNSYAAFAKAAPDVNGALLTGSDDAIQRALNDPL
                     ASYLNGDITEAEMYTQWLDAVRNEFPDLVIPEPPVTE"
     gene            1704..2642
                     /locus_tag="PLT08_11925"
     CDS             1704..2642
                     /locus_tag="PLT08_11925"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HNE05222.1"
                     /translation="MGLIKLKKHQYGYIFIAPFIIGFLAFGLYPVYNTLALSFTDATL
                     MTKSAHFVGFKNFERLFADDVFLGAVKNTWLLWALNFIPQVGIAMLLSVWFTDARLKI
                     KGVGVWRAVFFLLNLLVPASVAALFFAFFSFYGPVNQFMVRFGFLPEAMHYLQSVPVT
                     RGLVIFIQWWMWFGQTVIIVMAGMTSIPVSLYEAAMVDGATTSQMFRRITLPLLKPIL
                     IYIFVTSLVGGMQMFDIPFLLTDGRGSPNSSIQTNNILMYMKFASSKGHIGAASSVGV
                     LVFLMTSICALGIFYFLRERDTVSEKPKKSLFGKRS"
     gene            2626..>2852
                     /locus_tag="PLT08_11930"
     CDS             2626..>2852
                     /locus_tag="PLT08_11930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE05223.1"
                     /translation="MENVRESDKAMLNNYKFTTTVLRILVYFMLFALLVVTIVPIWLL
                     IVNATRSTTEIQQGLSIMPSTHLIENYNILLG"
BASE COUNT          675 a          709 c          675 g          793 t
ORIGIN      
        1 tgttactcgc aaatttcatg tacttgaaat gcgagcgtcc gatatctgaa tgacatcgga
       61 cgccaagaag tcgaagcccg actctatgga aggatgcgga cttcaacttg gaaatatacc
      121 accataatta ttaacaagga gaacgaagat gaaacgatta tcttacatct tatctctgct
      181 ggttgtcgtt agcatgttct tagcagcatg cgcaccagca gcgactgaag aagcccccgc
      241 tgccactgaa gcagccgctg aagctactga tgcccctgca gccactgaag ctgcccctgc
      301 tgaattatct ggtactttga atgtttggtc gttcaccaat gaaattcgca ccatggcgat
      361 cgcattcgaa ggtgttcacc cgaatgttga tgttgtttac accatgatcc cgatgaccaa
      421 tggtgaatac cagaccaagg ttaaggctgc tgccggtacc gcggattccc ccgatgtggt
      481 cgcgcttgaa gcggctttcg tcaaggaatg ggttgaatct gatcttttgg ctgaccagag
      541 cgacttgctg cctttggctg aagaactcaa gaccttcccc tctgttgtag aagttggtac
      601 gtatgatggc atcaccaagg cgtattctta tcaggcgacc cccggcgcgt tcttctatcg
      661 ccgcagcatt gccaaggaat gcctcggcac cgacgatcct gatgctgtgc aagccatggt
      721 tgctgacctc gataagttcg ttgaaaccgc tgccaagatc aaagaatgcg gcaccggcga
      781 ttacttcacc gtgggtacct ctggtgaaat gttcaacccc ttcctcgcca atcgcgaaca
      841 gccctgggtt gtggatggtg aactcgtgat cgacccgatg gtcttggaat atgtcaagtt
      901 tgccaagctc atgcgcgaga acggctacga atctcaggct actcagtgga gtgaaggttg
      961 gttcgctggc atgaacgata ctttgaagga tgccaacggc accccgaaga aggtcttcag
     1021 ctacttcctc cccacttggg gtcttcctta cgtgttgatc cccaactcca agtctgacac
     1081 caccgacacc ggcggcgact gggcgatgat caatggtcct atggcgtatc aatggggtgg
     1141 tacttgggtt ggcgctttgg aaggcagccc caatgctgaa ttggctaagg aattcatcaa
     1201 gttcgtagct cttaacgaag ataacctgac caactgggca accggcgttt acaccaacga
     1261 atacctcaag gcgatcgatc ctgaaacccc agatgatcaa gctcaggctc ctggcgactt
     1321 tgtttccagc caggttgtgg ttgagaagat caccagcacc ttcgatggtt cagatcttgc
     1381 caaatggctc ggcggacaga actcctatgc cgctttcgcc aaggctgctc cagatgtcaa
     1441 cggtgctttg ttgaccggtt ctgacgatgc cattcagcgt gccctcaatg atccgctcgc
     1501 cagctacctc aatggcgaca tcactgaagc tgaaatgtac acccagtggc ttgatgcagt
     1561 tcgcaatgag ttcccggatc tcgttatccc tgaacctcct gtcactgaat aagagaatgt
     1621 gaagtagtgg ttgggtcagt gcaaaacact gacccaaccc gtcagaacac cactgacaat
     1681 ttttattttg ttgaggagac gtcatgggac ttatcaaact taagaaacat caatacgggt
     1741 atatttttat tgcaccgttc attattggtt ttcttgcctt cggcttatac ccggtctata
     1801 acactttggc attgagcttt actgatgcaa ctctaatgac caaaagtgct cattttgtag
     1861 gtttcaaaaa ttttgagcgg ttgtttgctg atgacgtctt ccttggtgct gtaaagaata
     1921 cctggttact ttgggcgctg aactttattc cacaggtcgg gatcgccatg ttgttatccg
     1981 tatggttcac cgatgcaaga cttaagatca aaggtgtggg ggtatggcgg gcggttttct
     2041 ttttgctcaa cctgctggta cctgcatctg tagcggcttt atttttcgcc ttcttttctt
     2101 tttacggacc tgtcaatcaa tttatggtgc gttttggttt tttgcccgaa gccatgcatt
     2161 acctccaaag tgtaccggtt acgcgcggat tggttatctt cattcaatgg tggatgtggt
     2221 tcgggcaaac tgtcatcatc gtcatggcag ggatgacgtc catccccgtt tcattatatg
     2281 aagctgccat ggtagatggc gcaaccacca gccaaatgtt ccgtcgtatc actctgcctt
     2341 tgctcaagcc cattttgatc tatatctttg tcacatctct tgtgggcgga atgcaaatgt
     2401 tcgatattcc tttccttctc acggatggaa gaggctcacc caatagctcc attcaaacca
     2461 acaacatctt gatgtacatg aagttcgcca gtagtaaggg acatattggt gcagcttcat
     2521 cggttggtgt gctggtattt cttatgacca gcatttgtgc actgggaatt ttctacttct
     2581 tgcgcgaaag agatacagtt tcagaaaaac ccaagaagtc tctctttgga aaacgttcgt
     2641 gaaagtgata aagccatgtt gaacaattac aaattcacaa ccaccgtcct tcgaattctt
     2701 gtttatttta tgctgttcgc gttactggtc gttacgatcg tgccgatctg gctattgatc
     2761 gtcaatgcaa cccgctcaac caccgagatc caacagggtc tcagcattat gcccagtacg
     2821 catcttattg aaaattacaa catcctcctt gg
//
LOCUS       DAOQJE010000467         2852 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_24086_length_2852_cov_5.582052, whole genome shotgun sequence.
ACCESSION   DAOQJE010000467 DAOQJE010000000
VERSION     DAOQJE010000467.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2852)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2852)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2852
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24086_length_2852_cov_5.582052"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..697)
                     /locus_tag="PLT08_11935"
     CDS             complement(<1..697)
                     /locus_tag="PLT08_11935"
                     /inference="COORDINATES: protein motif:HMM:TIGR04214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CSLREA domain-containing protein"
                     /protein_id="HNE05224.1"
                     /translation="MNHLTVTRIFSVFAICLSLLVSSPPKSVQAQSTTITFKVNTTDD
                     RVDANPGDGVCATSNPVRCTLRAATMEANNIHTATNTSILIVLQPTTYTLTLKNNNIG
                     VPLSTVTDTNIQVTIEGTNGSATIQGGAGWNNQIINTASVSGQSSSQTSLRNLIIQNG
                     TTGIVGGGVFENVTIRNNISGGGITVTTPLTCLNCVISNNSKIGDGGGITVNAALTCV
                     NCVISNNSATGNGG"
     gene            complement(982..>2852)
                     /locus_tag="PLT08_11940"
     CDS             complement(982..>2852)
                     /locus_tag="PLT08_11940"
                     /inference="COORDINATES: protein motif:HMM:NF024977.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNE05225.1"
                     /translation="SSRKLQYADRVAIQQADQAIRKDVFRALVEIITNSNDSYLRLEQ
                     AGWSVSGEIYIDVLRKHKNSIIRIWDNAEGMNDSRMDKVVGTYGEATSGIKEDKNVRG
                     MWGRGLKDSIFGLGHGYVRSFKGGNFYSCSLLIKNGIPTFSLDEPTRALVPIRQQYGI
                     LEGNGTVIEIIVSRDDVKVPQFDNFRNYLQRHFELRPIMSNPKRKIILREMDSDWNVK
                     QEHVLSYKAPKGVKVLSERVKIPNFPAWVKLELYRSSIQLSMRGEEGDYADGGLLVIS
                     KGMVLSLTMLKYENDPLAAYFYGSMQCDYLHDLLKNDEPVLTATRDGINWSHPFAKAL
                     RQAVEEKIEPLIEKERKHALREEQTKLDKQLRQKIDKALHELNHIALNELSNRKEGES
                     RIQLPASGIMFFPERAYVQTGRTLSLILRADITKKAKPNAVVEITSNSPEIIVLTKQV
                     KLKPHKADPSVGEAIVRIEGRQVGGEGLVIARVNKMHAQAMVQVHSKKEVLVEPPARS
                     KNMLFNDIRFDDRMDPRQRVYFDRVNSSIVIATNAPSVKMYLDENKRLDTSMQGQVLL
                     AELVTEAVCREIAREGVEKGRFLVLEGSEADAIQNHFIRLQNRYSHLIHEHIVTIG"
BASE COUNT          710 a          684 c          648 g          810 t
ORIGIN      
        1 caccgccatt tccagttgca gagttgttag agatgacgca attcacgcat gtaagtgccg
       61 cattaacagt aatcccacca ccatcaccga ttttggaatt atttgaaata acacagttca
      121 agcaagtgag aggggtagtt acagtaattc caccacccga aatattattt cgaattgtga
      181 cattctcaaa aacaccaccg ccgacaattc ctgtagtgcc attttgaata attaggtttc
      241 gcagggatgt ttgactacta ctctgccctg aaacagaagc cgtgttaata atttgattat
      301 tccagccggc gcctccttga atggtggcag agccattagt tccttctatg gtgacttgta
      361 tattagtatc cgttacagtg ctcagcggga caccaatgtt gttatttttt aaggtcaagg
      421 tataggttgt aggttgcagg acaataagga tagaggtgtt tgtagctgta tggatattgt
      481 ttgcttccat ggtcgctgct cgtaatgtgc agcgtacagg attgctggtc gcacaaactc
      541 catctcctgg gttggcgtct accctgtcat cggttgtgtt aactttgaat gtaatggttg
      601 tgctttgcgc ctgcactgac ttgggcggac tagaaaccaa taaactaagg caaattgcaa
      661 aaacggaaaa aatccttgta acagtaagat ggttcatatc ttttctcctt gttatataat
      721 taataaacaa agaaaataat atgatcaaac aacaaggata agtagcgcct gtagtattaa
      781 tattatcgtt cgttggttag gaaagaacac taactaaagt tggtgcgacc gctaagatac
      841 gaatagattt ctgaaaggct gtatgtagta tagacaatcg ttttgctttc ccaatttgaa
      901 tactttatta aatcgtttta taaaaaccct caaaagacaa aggacggcag accgtaagtc
      961 tgccgtcctt tgtctatcgt ctcacccaat tgtcacaata tgttcgtgga tcaaatgtga
     1021 gtagcggttc tgcaagcgga tgaagtggtt ttggatcgca tccgcttctg agccttcgag
     1081 gacgaggaaa cgtcctttct ctacaccttc gcgggcgatt tcgcggcaca cggcttcagt
     1141 gacgagttct gctagaagca cttgcccctg catggatgtg tcgagccgct tgttttcatc
     1201 cagatacatt tttacagagg gggcattggt tgcgatcaca atgcttgagt tgacacggtc
     1261 aaaatacaca cgctgacgtg ggtccatgcg atcatcgaag cggatgtcgt tgaagagcat
     1321 attcttgctg cgtgcgggcg gctccacgag cacttctttc ttcgaatgga cttgcaccat
     1381 ggcttgtgca tgcatcttgt tgactcttgc gatgaccaac ccttcaccgc ccacttggcg
     1441 accttcaata cgcacaatcg cttcgcccac gctagggtcc gctttgtgag gtttcaattt
     1501 cacttgtttg gtcaacacaa taatttcggg gctattagat gtgatctcca caaccgcatt
     1561 cggcttggct ttcttcgtaa tatctgctcg cagaatgagc gaaagcgtgc gccccgtttg
     1621 cacataagcc cgttcaggga agaacataat gcccgaagct ggcaattgaa tgcgagactc
     1681 accttctttg cgattgctca actcgttcaa cgcgatatga ttcaattcat gtaacgcttt
     1741 atcgatcttt tggcgcagtt gtttgtcgag cttggtttgc tcttcgcgta gggcatgctt
     1801 gcgttctttt tcgatcagcg gttcgatctt ctcttccact gcctgtcgca gcgccttggc
     1861 aaagggatgt gaccagttga ttccatcacg cgttgccgtg aggaccggct catcattttt
     1921 caacaggtca tgcagataat cacattgcat cgagccatag aaatatgccg ccaacggatc
     1981 attctcgtac ttgagcatgg tcagcgataa gaccatccct ttgctgatca cgagcagacc
     2041 gccatcggca taatcgcctt cttcgccgcg catggaaagc tgaatagaag aacggtataa
     2101 ctccaacttt acccaagccg ggaagttggg aatctttacg cgttcgctca gaaccttcac
     2161 acccttcggc gctttgtaac taagcacatg ctcttgcttg acgttccagt cagagtccat
     2221 ttcgcgcaag atgatcttac gcttggggtt gctcatgatg ggacgtaact caaagtgacg
     2281 ttgcaggtaa ttgcggaagt tatcgaactg cggcactttt acatcgtcac gcgaaacgat
     2341 aatctcgatt accgtcccat taccttcaag gattccgtac tgctggcgga tcggcactaa
     2401 ggcgcgagtt ggttcatcca acgaaaaggt tgggatccca ttcttgatga gcaaagaaca
     2461 ggaatagaaa tttccgcctt tgaatgaacg cacatatccg tgccccaaac caaagataga
     2521 atcttttaat ccgcgtcccc acatgccgcg cacattttta tcttccttaa taccgctggt
     2581 cgcttcgccg taggtgccca ctactttatc catgcgcgag tcgttcatgc cttcggcatt
     2641 gtcccaaatg cgaatgatgg aattcttatg cttacgcagc acatcaatat aaatttcgcc
     2701 gcttacagac caaccggctt gttcaaggcg caaataactg tcgttgctat tggtgatgat
     2761 ttccaccagc gcgcggaaca catctttgcg aatcgcctgg tcagcctgct ggatcgcaac
     2821 gcggtctgcg tactgtaatt ttctactact gc
//
LOCUS       DAOQJE010000468         2847 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_24159_length_2847_cov_4.766476, whole genome shotgun sequence.
ACCESSION   DAOQJE010000468 DAOQJE010000000
VERSION     DAOQJE010000468.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2847)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2847)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2847
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24159_length_2847_cov_4.766476"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1024)
                     /locus_tag="PLT08_11945"
     CDS             complement(<1..1024)
                     /locus_tag="PLT08_11945"
                     /inference="COORDINATES: protein motif:HMM:NF018012.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0015948 - methanogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trimethylamine methyltransferase family protein"
                     /protein_id="HNE05226.1"
                     /translation="MNNVRPRLSLMNEEQIQEAHRNVLKVLSETGVRVDSPEILQMLE
                     WKLGLKSQDRIIKFPPAVVEEAIKSAPKTIDVYDRRGEHKLKLGEDRLRFGVGVTALF
                     YQNPVDETLDIFKRENFRDLVRLASGLKHYDVISTVGIVRDVKEELGDMYGSLEHISN
                     TTKPLVLLVSDEDRFPDVLDMFEHLNGKEVGDKPFVIPYFNPVSPLVMNAGTVDKMKV
                     AIERGLPIIFSNYSMAGASTPLTPAGTLTLLMAELLAGLVISQTIKKGAPILLGMLPV
                     YFDMRTMLNFYDPQSILISVACSEMMKFYGIPHCSTSGSGTGWGMDLIAADTYWMNTL
                     ALLLSNG"
     gene            complement(1099..2112)
                     /locus_tag="PLT08_11950"
     CDS             complement(1099..2112)
                     /locus_tag="PLT08_11950"
                     /inference="COORDINATES: protein motif:HMM:NF013341.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RIO1 family regulatory kinase/ATPase"
                     /protein_id="HNE05227.1"
                     /translation="MSKNQLHDYFDELDDLSEGAQDSARKRTSIKRESKRKEGEAKLF
                     IRAQEAAKEFKFTYKAARFEEAWLLDSLTEIYEHQWISDVLRKVKGGKEASVYLCKSG
                     TEMSVPFVAAKIYRPRSLRNLKNDAQYRVGRVDLDADGSALWKEADINAIAKRTTYGE
                     EVRHRSWIAYEFKTMEKLFEAGVDVPKPYAMEKNAIVMDFIGDANTAAPILNSVNLSA
                     HEAKNLFDRVIHNIDLMLANDCIHGDLSAYNILYWNGAITLIDFPQIVLPKSNPTAWN
                     IFQRDVIRICDYFSAQGIHCDGRKIASELWTSRGYRIGKQADPKYLDPEDPADRKLWE
                     KQQ"
     gene            complement(2359..>2847)
                     /locus_tag="PLT08_11955"
     CDS             complement(2359..>2847)
                     /locus_tag="PLT08_11955"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HNE05228.1"
                     /translation="PDPVCRGSELQGGRELLPGIIALLIGALISAEGNIIFKIYSVNS
                     NPVSINALSLFSAAVFLGIASFFTQETWNLPSTSAGWTALAYLIIFGSVLMFYLFVYV
                     LTRWTASSASYSILLFPLVATVFAALLANETITLPFILGGVIVLLGVWVGAFYTKPKT
                     GA"
BASE COUNT          743 a          671 c          743 g          690 t
ORIGIN      
        1 gtccgttaga gagaagaagc gcgagggtat tcatccaata ggtgtctgcc gcgataaggt
       61 ccatacccca gccggtgccg ctgccagaag tggagcaatg cggaatgccg tagaacttca
      121 tcatttcgga acaagcaaca ctaatgagaa tactttgcgg atcatagaag ttgagcatag
      181 tgcgcatgtc aaaatagaca ggcaacatgc caagcaaaat tggtgcgcct tttttgatag
      241 tttgactaat gactaaacct gcaagcaatt ctgccatcag gagcgtgagc gtgcctgctg
      301 gcgttaatgg tgtggatgcg cccgccatgc tgtagttgga aaagatgatg ggaagcccgc
      361 gttcgatggc gactttcatt ttgtcgacgg tgccggcatt catcacgagc ggactgaccg
      421 gattgaaata cggaattaca aagggcttat cgccgacttc cttgccattc aaatgctcga
      481 acatatcgag cacatctgga aagcgatctt catctgagac aagcaacacc aatggcttgg
      541 tggtattgga gatgtgctcc aacgagccgt acatatcgcc aagttcttct tttacatcac
      601 ggacgatccc caccgttgag atgacgtcat aatgtttgag cccagaagcc aagcgtacaa
      661 ggtcgcgaaa gttctcgcgt ttgaaaatat ccagcgtttc atccaccggg ttttgataga
      721 acagcgccgt cacccccacc ccaaagcgga ggcggtcttc ccccaatttc agcttatgtt
      781 cgccgcgacg gtcgtaaaca tctatcgtct ttggcgcgga cttgatcgct tcttcgacaa
      841 ctgcgggcgg gaatttgata atgcggtctt gcgacttcaa gcctaatttc cattcaagca
      901 tttgcagaat ttcaggcgag tcgacgcgca ctccggtttc gctgaggact ttcaacacat
      961 ttcgatgtgc ttcttgaatc tgttcttcat tcatcaaact caatcgcggg cggacgttgt
     1021 tcatagatca tctcctattc aaattgtaat agaggaagga agaaaaaaga agccagtgca
     1081 attgcactgg ctttgtactt actgttgttt ctcccacagt ttgcggtctg cggggtcttc
     1141 ggggtcgagg tatttggggt cggcttgttt gccgatgcga taaccgcggg aagtccaaag
     1201 ttcggacgcg attttacgtc catcacaatg aatgccttga gccgagaaat aatcgcagat
     1261 gcgaataaca tcgcgttgaa aaatgttcca tgcggttggg ttggatttcg gcaaaacaat
     1321 ctgcggaaag tcgatcaacg tgatggcgcc gttccagtac aaaatgttgt atgcggataa
     1381 gtcgccatga atacagtcgt ttgccaacat caggtcgatg ttatggatca ctctgtcgaa
     1441 caagtttttg gcttcatgtg cagaaagatt aaccgaattc aagatcggcg cggctgtgtt
     1501 tgcatcgcca ataaaatcca tcacgatggc attcttttcc atggcatagg gtttggggac
     1561 atcgacaccg gcttcgaaaa gcttttccat ggtcttgaat tcgtaggcga tccacgaacg
     1621 atggcggacc tcttcaccgt aggtagtgcg tttggcgata gcgttgatat cggcttcttt
     1681 ccacaaggca gagccgtcgg catccaagtc cacgcgcccg acgcggtatt gcgcatcatt
     1741 cttgaggttg cgcaacgaac gcggacggta gatcttcgcg gcgacgaaag gcacactcat
     1801 ttcggtgcca gacttgcaca agtagaccga ggcttccttg ccgcctttga cctttcgcaa
     1861 tacgtcagaa atccattggt gttcgtagat ctctgtcagc gaatccaaga gccaggcttc
     1921 ttcgaagcgc gcggctttgt aggtgaactt gaattctttg gcggcttcct gggcgcggat
     1981 gaagagcttc gcctcgcctt ctttgcgttt ggactcgcgt ttaatagaag tacgcttgcg
     2041 agccgagtct tgtgcacctt cactcagatc gtctaattca tcaaaatagt catgtaattg
     2101 attcttactc attgttataa aaccttatct cttaacaaaa aaaccacagg gaccttcctg
     2161 cggctggttg aatgaggtta aacaaaaacc gcaggctgcg agcctgcggt tgaatcgata
     2221 gggcaagact atgccaattc gctcaacgga ggcgcgccaa acaaaacaac tgtggaaacc
     2281 aagatagctt gcatgttttt gagcctcctt tcgtggaata agatagtaaa gaaattttat
     2341 gccaagcccc aagcaacgtc aagccccagt tttgggtttg gtgtagaaag cacccaccca
     2401 caccccaaga aggacaatga ctccgccaag gataaagggc aaggtaatcg tctcgtttgc
     2461 tagcagggcg gcgaagacgg tagccaccaa agggaaaagc aaaatggaat aagaagcgga
     2521 agaagccgtc cagcgagtga ggacgtagac aaacaggtag aacatgagca ccgagccgaa
     2581 gatgatgaga taggcaaggg ctgtccagcc ggcggaagtg gagggcaaat tccaggtttc
     2641 ttgggtgaaa aacgaagcga tgccaagaaa taccgcggca gagaacaagg acagggcatt
     2701 gatcgatacg gggttgctat tgactgaata tattttgaag atgatgttcc cttcggcact
     2761 gatgagagcc ccaatgagta aggcaataat gccgggaagt agttctcgcc cgccttgaag
     2821 ttcggatcct cgacatacgg gatcggg
//
LOCUS       DAOQJE010000469         2846 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_24172_length_2846_cov_5.581154, whole genome shotgun sequence.
ACCESSION   DAOQJE010000469 DAOQJE010000000
VERSION     DAOQJE010000469.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2846)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2846)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2846
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24172_length_2846_cov_5.581154"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(165..>2846)
                     /locus_tag="PLT08_11960"
     CDS             complement(165..>2846)
                     /locus_tag="PLT08_11960"
                     /inference="COORDINATES: protein motif:HMM:NF013124.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HNE05229.1"
                     /translation="TFGGWLRKRRRELDLTQQTLAERAGCTRITLRRVEAGTLKPSEH
                     LAETLLKLLGIPSQERKAWVQFARGIADHPTFAQFLLRTNLPSLLTSFVGRKKEISEV
                     KDALFNHRLVTLTGSGGIGKSRLSLQVAADLLTDFLDGVWFIELASLSDPNLLPQTIQ
                     NILGLIEQKNTSPLQTLQEYLKEKKILLVLDNCEHLVEACATVAHTLLSHTTSLKILA
                     SSREALGVQGEFAWRVPSLSVPDTDKGVEADQLSRYEAVKLFIERARLIDPQFVLDET
                     NASFVARICSRLDGIPLAIELAAARLKTLTVEQIHARLDDRFSLLTNGARTLPSRHQT
                     LRAAIEWSYTSLSENEKTLFRRLAVFTGGWSLEAAETICSGNGIKQEDVLDLITSLLQ
                     KSLIHTSEFRPGVRYEQLETIRQYGQEKFLASDEVEILRNRHLQYFVELAKRAEPELR
                     SSAQVQWFTLLEQEISNLRSAMQWAKETDKTAFLQLTSSLWLFFRSLEHKDEGIDCLL
                     KAVSVNKDTPTPLLSTAMARLASLSIYLSIVQDQTEDYALTALDLSRQVRDKPAEALA
                     LISLAGLELERMTGKFGPEYTELALGIARDLKDSWLISTALIQKGRFTQIKNPIESVA
                     IFEEALKEAQASGDKRLLYSNLFWMFINILATGQLARAKEIAHQCVLVATEIGDKDGI
                     MYSHNALAGIGLFEEDYESSKEHAETIIRLSKAYQHNSGLLNGLGTAGLVNLATKNIS
                     QVLELSTEMDRLILSKSGRLKSDTGYSVFLRIWAYILERDLKNARSNARELIPLYTQN
                     DNILLSIEYFRVFAALAYMDGCHEINITLTSFAKRLHERVALAYFDYPFMSRLRAEQL
                     AQSREALGADAFNQAWEKGQQLDLEEAKKYTLEIANR"
BASE COUNT          729 a          668 c          671 g          778 t
ORIGIN      
        1 ttcctatatc acattagtca cttgttctgg gtacaacccg ctcaatgaaa gttatttctt
       61 tagaagaata gttcgagccg tgctggtcag cacggttagt gagtaacaaa acgagacctc
      121 cgaagtctta aagacttcgg aggtcttttt tatgagcaag ggcattatcg gttggcgatt
      181 tccaatgtgt atttttttgc ctcttccaag tccaactgct gtcctttttc ccaagcttga
      241 ttaaaagcat cggctcccaa ggcttcgcgt gattgggcta gttgttctgc ccgcagcctg
      301 ctcataaatg gataatcgaa gtaggctaaa gcaactcgtt cgtgcaatct cttggcaaag
      361 ctggtcaggg ttatatttat ttcatggcag ccatccatat atgccagcgc cgcaaaaacc
      421 cggaagtatt caatggataa aaggatgttg tcattctggg tatacagggg gatgagttct
      481 ctggcattac ttctggcgtt tttcagatca cgttcaagga tataagccca gatgcgaaga
      541 aacacagagt aaccggtatc agacttaagg cgaccactct ttgaaaggat caacctatcc
      601 atttcagtag atagttcgag tacttgtgaa atattttttg tcgccaaatt taccagcccg
      661 gcggttccaa gcccatttag tagaccagaa ttgtgctgat aagcttttga taatcgaatg
      721 atggtctcgg cgtgttcttt ggaggattca taatcctctt caaataaacc aatacccgcc
      781 aaggcattat gagaatacat aatgccgtcc ttatcgccga tctctgttgc aaccagtaca
      841 cattgatggg caatctcttt tgccctggca agctgccccg ttgccagaat attgataaac
      901 atccaaaata aattggagta cagcaggcgc ttatcgccag atgcctgagc ttctttgagt
      961 gcctcttcaa aaatagctac gctttcaatc ggattcttta tttgggtaaa tctccccttc
     1021 tgaatcaaag cagtggagat caaccaagag tctttgaggt cacgtgcaat tcccaatgcc
     1081 agctcggtat actccggacc gaactttccg gtcatgcgtt ccaattccaa gcctgccaat
     1141 gaaattaacg ccaaggcttc agcgggttta tctcttacct gcctgcttag gtcaagtgct
     1201 gtaagggcat agtcttccgt ttgatcctgc acaatagaaa ggtagatgga aagagaagct
     1261 agccgcgcca tggcagtcga aagtaaagga gttggtgtat ctttgtttac cgacacagct
     1321 ttcaacaaac agtcaatgcc ttcatcttta tgttccaatg acctaaaaaa gagccaaagg
     1381 gaactcgtca actgaaggaa ggcggtttta tccgtttctt ttgcccattg catggcagag
     1441 cgcaggttgg atatttcctg ttcgagaagg gtaaaccatt gcacctgcgc ggaactacgc
     1501 aattctggtt ctgcgcgttt tgctaactct acaaaatact gcaagtgccg attacgaaga
     1561 atctccactt catcactagc taagaatttt tcctgcccat actggcggat ggtctctaat
     1621 tgttcatagc gaactcctgg gcgaaattca ctggtatgaa tgagagactt ttgtaatagg
     1681 ctggtgatca ggtcgagcac atcttcctgt ttaattccat tccctgagca aatggtctcg
     1741 gcggcttcca aactccagcc accggtaaac actgccaacc ggcgaaataa ggttttttca
     1801 ttctccgata gggatgtgta gctccattca atagctgccc gcagggtttg gtgacgggaa
     1861 gggagagtcc gcgcgccgtt agtgagcaaa ctaaagcgat catcaagccg agcatggatc
     1921 tgttccacgg ttagggtctt caatctggca gcagccaact caatcgcaag aggaatgccg
     1981 tcgaggcggg aacagattcg ggcgacgaaa gaggcgttgg tctcatccaa aacgaattgt
     2041 ggatcgatca agcgcgcccg ttcgatgaat aacttcaccg cttcatatcg tgacagttgg
     2101 tctgcctcta ctcccttgtc tgtatctggc actgacagtg acggaacgcg ccaggcaaat
     2161 tcaccttgta cccccaacgc ctcgcggctg gatgccagaa tcttaaggga ggtggtatgg
     2221 ctcaacaagg tatgggctac tgttgcacag gcttcaacca ggtgttcaca gttatccaac
     2281 actaataaaa ttttcttttc cttcaagtat tcttgaagtg tttgtagggg agatgtattt
     2341 ttttgttcga tcaaccccaa tatgttctga atggtttgcg ggagcaggtt ggggtctgag
     2401 agagatgcca attcgataaa ccaaacacca tcaaggaagt ctgtgagcag gtctgcagcg
     2461 acctgcagag ataggcgcga ttttccaatt ccaccagagc ctgtgagggt tacaaggcga
     2521 tgattaaaca gtgcatcttt gacctcagat atttcttttt tgcgcccgac gaaactggtc
     2581 aacagggagg ggagattcgt tcttaacaga aattgtgcaa aagttgggtg atctgctata
     2641 ccccgtgcaa attgaaccca tgccttgcgc tcctggcttg gaatgccaag aagtttaagc
     2701 agggtttcag ccagatgctc tgatggtttc agtgttccag cttcgactct acgcaatgtg
     2761 atgcgggtac acccagccct ctctgccagc gtttgttggg tcaaatctaa ttcacggcgg
     2821 cgttttcgca gccacccccc aaaggt
//
LOCUS       DAOQJE010000470         2846 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_24173_length_2846_cov_4.768900, whole genome shotgun sequence.
ACCESSION   DAOQJE010000470 DAOQJE010000000
VERSION     DAOQJE010000470.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2846)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2846)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2846
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24173_length_2846_cov_4.768900"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            192..1283
                     /locus_tag="PLT08_11965"
     CDS             192..1283
                     /locus_tag="PLT08_11965"
                     /inference="COORDINATES: protein motif:HMM:TIGR04474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="three-Cys-motif partner protein TcmP"
                     /protein_id="HNE05230.1"
                     /translation="MTKPNETLWEIEPHTKAKHDILQNYLGAWFGILGSKIPKIVYID
                     GFCGPGRYKGGEEGSPIIAVKEAMKHLSLLAQSNVTFLFIDERQDRIEHLKSELASLN
                     PPGNFHLDPRVNEFENTLTQILDGLAQSGRQLAPTFAFIDPFGFKGASFSVTRRLLNN
                     KSTEVFINIMVNFVNRFAKHPDATDRAHIKSLLGATDAEIDEVVSSPDRIEGFRQLYQ
                     SKLRQYARFVRFFEMRDQNNKVIYYLFFAGNHPKGHEKMKEAFWRVDRQNGYKFSDRT
                     DPNQPVLFEMDPSLELARILKTQYSGMNKLSQDVITFVVDETAYISTHCRRALAHLEN
                     NSEIRVESNKVDGKKRIKGTFPPGVVIHF"
     gene            1334..2059
                     /locus_tag="PLT08_11970"
     CDS             1334..2059
                     /locus_tag="PLT08_11970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage Gp37/Gp68 family protein"
                     /protein_id="HNE05231.1"
                     /translation="MAHSKIEWTEATWNPLTGCNKISPGCKHCYAERMAKRLQAMGQP
                     NYVNGFKLTMHPQSLEKPLEWKTPQIIFVNSMSDLFHKNVPLDFIQQVFDVMKRAHWH
                     QFQVLTKRSERLLDVSSKLEWTKNIWMGVSVENSDYTFRIDHLQNTGAKIKFLSVEPL
                     LGPIPKMKLDGIDWVIVGGESGPGARPLEREWVVDIKNQCVKARVPFFFKQWGGVQKK
                     KAGRLLDGRTWDQMPMEMDFVNA"
     gene            2232..>2846
                     /locus_tag="PLT08_11975"
     CDS             2232..>2846
                     /locus_tag="PLT08_11975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE05232.1"
                     /translation="MHIAAPINTSHYIQIIEGNLNAKSMDFARLPGGEAHEGNPAWFL
                     SGLKRAGYNGIVRANLPARQLDTHIEKALEPFRQLDMPMTWWVDSQSEPAQLGRALQN
                     HGLHHLRDMFGMAAPLDSLPAVVAPDMEHSFEAVQSRHGLEEWLPLFMDTFDVPQADN
                     ALVLDVFDRLSFGPHAQWHHFLIRVEGRVVATGSLHLGAGVAGLY"
BASE COUNT          836 a          597 c          657 g          756 t
ORIGIN      
        1 atggaggcgg caaattgttc ttgaggttca ggcatgggcg gcatgataca aaaagacacg
       61 ggcgggtatc ccgtccgtgt cgctttccct gtgaacaggg atataattcg atttattagg
      121 cggtaaaact acttcaaaac aattgccgcc accttgtcaa cactcagaca gaccatgtta
      181 gggagacata aatgacaaag ccaaacgaaa cattatggga aatcgaacca cataccaagg
      241 caaaacatga tattctccaa aactatttgg gggcttggtt tggtattctg gggtcgaaga
      301 tcccaaagat tgtgtatatt gatggctttt gcggaccagg gcgttataag ggtggggaag
      361 aaggatctcc catcattgcc gtgaaggaag caatgaaaca cttgtctctc cttgcacaat
      421 caaatgttac atttcttttc atagacgaaa ggcaagatcg catagaacat ctgaaatccg
      481 agctggcatc tttgaaccct ccaggcaatt ttcatctgga cccgagagtg aatgagtttg
      541 agaatacact tacccagata ctggatgggc ttgcccaaag tggacgtcaa ttagccccca
      601 cttttgcatt cattgatccg tttggattta aaggtgcatc attttcggtg acgcggaggt
      661 tgctgaacaa taaaagtaca gaagttttta ttaatattat ggtcaacttt gtaaaccgtt
      721 ttgcaaagca ccccgatgca acagacagag cgcatattaa aagtttactc ggtgcaacgg
      781 atgctgaaat tgatgaggtt gtatcctcgc ctgatcggat tgaaggcttc cgccaactgt
      841 atcaaagtaa acttcgtcaa tatgccagat ttgtgcgttt ctttgaaatg cgcgatcaaa
      901 acaataaagt aatctactac cttttctttg cgggaaacca tccgaaagga catgaaaaaa
      961 tgaaagaagc cttttggaga gttgaccgtc aaaatgggta taaattttct gacaggaccg
     1021 acccaaatca acctgttttg tttgagatgg atccatccct cgaacttgca cgaattttaa
     1081 aaactcaata ttctggaatg aataaattat cccaagatgt gattaccttt gttgtggacg
     1141 aaacagctta catctcaact cattgcagaa gggcgttagc tcatcttgaa aacaattctg
     1201 aaattcgagt tgagtcaaac aaggtggacg gaaaaaaacg aataaaaggg acttttccgc
     1261 ctggagttgt tatccatttt taatataaaa atagaacgtt tgtactatta ttggtagtac
     1321 accggaggaa gaaatggctc attcgaaaat tgagtggaca gaggcgacct ggaatccact
     1381 aacaggctgt aacaaaataa gccctggttg taaacactgt tacgcagagc gaatggcaaa
     1441 acgattacaa gctatggggc aacctaacta tgtaaatggt tttaagttaa ccatgcaccc
     1501 tcaatcacta gaaaaacctt tggaatggaa aactccgcag attattttcg ttaattcaat
     1561 gagcgacttg tttcataaaa atgtacctct ggattttatt caacaggtat ttgatgtgat
     1621 gaagcgcgct cattggcatc aatttcaggt tttaacaaaa cgttccgaac gattattgga
     1681 tgtaagttca aaacttgaat ggaccaaaaa catctggatg ggtgtaagtg tcgaaaattc
     1741 tgattacaca ttcagaattg accatctgca aaacacgggt gcaaaaataa aattcctctc
     1801 cgtcgagcct cttttaggtc ctatcccaaa aatgaaatta gatgggattg attgggtaat
     1861 tgttggcgga gaatcaggtc ctggagcacg tcctctcgaa agagaatggg ttgttgatat
     1921 aaaaaatcaa tgcgtcaagg cgcgagtacc tttctttttc aaacaatggg gtggggttca
     1981 gaagaaaaag gctggaagat tgttggatgg cagaacctgg gatcaaatgc caatggagat
     2041 ggattttgta aatgcatgaa cttaatagac aacaaatttt gttggctatt tttagtttca
     2101 atcttaattc taatatcccc cacacttgat gcaattggag aattccgacc cattatccac
     2161 aagctaacaa tttcataaag cgcaaaccgc tttctggaag cgtatacttg ccggcatcaa
     2221 ggatcgttcg catgcacatt gcagctccca taaacacatc ccactatatt caaataatcg
     2281 aaggcaacct aaacgcaaag tcaatggatt ttgcccgttt accaggtggt gaagcccatg
     2341 aaggcaatcc tgcctggttt ctttccggct tgaaacgagc cgggtacaac gggattgtcc
     2401 gtgccaacct gcctgcccgg caattggaca ctcatatcga gaaagcattg gaaccgttcc
     2461 gacaattgga tatgcccatg acttggtggg tagattccca atctgaaccc gcccaactcg
     2521 gacgggctct acaaaatcac gggttgcatc atctgcggga tatgttcggc atggctgcac
     2581 ccctggactc cctgccggct gttgtggcac ctgatatgga gcattccttt gaagcggtgc
     2641 agagtcggca cggactggag gaatggcttc cgcttttcat ggacaccttt gatgtgccgc
     2701 aagcagacaa cgcgctggtg ctggatgtct ttgaccggtt aagtttcggt ccgcacgccc
     2761 aatggcatca cttcctgatc cgtgtggagg ggcgggtggt cgccacaggt tccctgcatc
     2821 ttggcgccgg ggtggcaggg ctttat
//
LOCUS       DAOQJE010000471         2831 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_24377_length_2831_cov_6.372118, whole genome shotgun sequence.
ACCESSION   DAOQJE010000471 DAOQJE010000000
VERSION     DAOQJE010000471.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2831)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2831)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2831
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24377_length_2831_cov_6.372118"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..733
                     /gene="panB"
                     /locus_tag="PLT08_11980"
     CDS             <1..733
                     /gene="panB"
                     /locus_tag="PLT08_11980"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003864 - 3-methyl-2-oxobutanoate
                     hydroxymethyltransferase activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HNE05233.1"
                     /translation="IDSILVGDSLAMVVLGYENTLPVTMDEMLHHARAVARGAKAPLL
                     VGDMPFMSYQVSVEDAVRNAGRFLQQGGMDAVKLEGGRERADAVRAIVNAGIPVMGHL
                     GLTPQSVNQLGGFRAQGKTASAAKRLLEDAQILEEAGAFSLVLESVPARLAEYISKHI
                     SIPTIGIGAGTGCDGQVLVTHDLLGLFERFTPKFVRQYAKLHETMNKAFTEYLNDVNT
                     KQFPAAEHTVEMTDEEWAAFQKEVK"
     gene            730..1626
                     /locus_tag="PLT08_11985"
     CDS             730..1626
                     /locus_tag="PLT08_11985"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: protein motif:HMM:TIGR00745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008677 - 2-dehydropantoate 2-reductase
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HNE05234.1"
                     /translation="MSENILLIGTGALSTLFAARLSEAGHHVSMLGTWKDGIQALNQN
                     GARVSESNGKEHAFQVHATDEPSEVRNVKHAIVLVKSWQTKRVAEKLKGILSPDGIVL
                     TLQNGLGNRETLARDLGAERVALGVTTTGATLLGPGHVKVGGEGILSLEQNQALGPLE
                     AALRSSNFNLQIVQNAQSLVWGKLIINAAINPLTALLQVPNGELLNHPWARKAMNALA
                     REAAEVAQAEQVNLPFNNPIEAVEEVARKTAQNLSSMFQDVRRGAPTEIDAICGAVTK
                     RGEMHGISTPYNRSCWQLVKAM"
     gene            1774..2676
                     /locus_tag="PLT08_11990"
     CDS             1774..2676
                     /locus_tag="PLT08_11990"
                     /inference="COORDINATES: protein motif:HMM:NF024645.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HNE05235.1"
                     /translation="MRKLFLLLLMGAFLSFPISASAQSDVSLSFATVQLWPEYDDPSM
                     LVIVDFQVAAATSLPATLTFRIPAEANLIAVASDTGNGQFLDHPYTGPNPEGEYQTFS
                     MVLEQNTPYRFEYYQPLSYNGDTRIFSYLWDSEYAVENFQYLFLEPLDVTDIALDPKY
                     TAKETFNGLNYYDGAPVQLAAGEQYSMKVSYTKTTDTLVSQAQSVQIAEPVNEDTPGR
                     VSLANSLPYIIGGLGIIMIAGGLMYYFQWGRHSAGGGKHRRRSRTNTESDATSIYCPQ
                     CGARAKPSDRFCRTCGARLRHDEA"
BASE COUNT          734 a          674 c          712 g          711 t
ORIGIN      
        1 gattgattcc attctggtgg gcgattcgct tgccatggtg gtactcggct atgaaaacac
       61 tctccccgtc accatggatg aaatgttgca tcatgcccgc gctgtagccc gcggagccaa
      121 agccccattg ctcgttggtg atatgccatt tatgtcgtat caagtttcag tggaagatgc
      181 tgtgcgcaat gccgggcgat tcttgcaaca aggtggaatg gacgcggtca agcttgaagg
      241 tgggcgtgaa cgagctgatg cagtgcgcgc cattgtcaat gcaggcattc ctgtgatggg
      301 tcacctcggg cttacgccgc aatcggtgaa tcaacttggt gggttccgtg cacagggcaa
      361 aacagcgtct gcggctaaac gtctacttga agatgcgcag attcttgaag aagctggggc
      421 gttcagcctc gtgctcgagt ctgtgcccgc aagattggcg gaatatattt caaaacacat
      481 ttccattcca accattggta ttggcgcagg gacaggctgt gatggacaag tgcttgtcac
      541 ccacgactta ctgggtctat ttgaacgctt tacaccgaaa tttgttcgcc aatacgcaaa
      601 gttgcacgaa acaatgaaca aggctttcac cgaatatctc aacgatgtga acaccaaaca
      661 atttccggct gccgaacata ccgttgaaat gacagatgaa gaatgggctg cttttcaaaa
      721 ggaagtgaaa tgagcgagaa tattttactt attggtacag gcgcgctctc tacattgttt
      781 gcagcacgtt tgagcgaagc tgggcatcat gtatccatgt tgggcacatg gaaagatggc
      841 attcaagctc ttaatcaaaa cggcgctcgt gtatcagaat caaacggaaa agaacacgcc
      901 ttccaagtcc atgctaccga tgaacctagt gaagttcgaa atgttaaaca cgccattgtc
      961 ttggtcaaat cttggcagac aaaacgtgtg gcagaaaaac ttaaggggat tctctcaccc
     1021 gatggaattg tgttgaccct gcaaaacggg cttggaaatc gcgaaacgct cgcacgggac
     1081 cttggggctg agcgtgttgc cctaggcgtt accaccactg gagccaccct gcttggacct
     1141 gggcacgtaa aagttggcgg cgagggtatc ctctcactgg aacagaatca ggctctcggt
     1201 ccgcttgagg cggctcttcg gtcatccaac tttaatttgc agatcgttca aaatgcgcaa
     1261 tccttggtgt ggggcaaact catcatcaat gcagctatta acccgttgac tgcgttgttg
     1321 caagtgccaa acggtgagtt gttgaatcat ccttgggcac gcaaggcgat gaatgctctg
     1381 gcgcgtgaag ccgcggaagt ggcacaagcg gaacaggtga atttgccatt caacaacccg
     1441 atcgaagcgg tggaagaagt ggctcgcaaa acggcgcaaa atctttcatc aatgtttcaa
     1501 gatgtacggc gcggtgctcc gacagagatc gatgccattt gtggtgcggt gaccaaacgc
     1561 ggagaaatgc acggaattag cacgccatac aatcgttctt gttggcaatt ggtaaaagca
     1621 atgtaattaa gtacgggcgg cgcatgcgcc gcccgtaccg atataaaaag ggtgacattt
     1681 atctatattc tgcatgattt cgtttctgcc ctcaaaaaat gcgacatggt aagatacata
     1741 tagaagtaaa acatttatcg ttaggaggcg ttcatgcgca aactattttt actccttctt
     1801 atgggagcct tcctctcgtt cccgatttcc gcatctgcac aaagcgatgt gtcgctttca
     1861 tttgccactg tgcaattatg gcccgaatat gatgacccca gcatgctggt gatcgtagat
     1921 tttcaagtag cggcagctac aagtttgcct gctacactca ccttccgtat tcctgcagaa
     1981 gccaacctca tcgctgtcgc ttcagataca ggcaacggac aattcctcga tcacccatac
     2041 acaggaccca accccgaagg tgaatatcaa acctttagta tggttcttga gcaaaatacc
     2101 ccctatcgtt ttgaatacta tcaaccgctt tcatacaacg gtgatactcg cattttctct
     2161 tatctttggg atagtgaata tgcggttgag aattttcaat accttttcct tgaaccttta
     2221 gatgtcaccg atattgcgct cgacccgaag tacactgcca aagagacgtt caacggattg
     2281 aactattatg acggtgcgcc tgttcaactt gcggcgggtg aacaatattc gatgaaagta
     2341 agttacacca aaactacaga tactttagtc tcgcaggcac agtcggtgca aatcgcagaa
     2401 ccggtgaacg aagatacgcc aggtcgcgtt tcacttgcca atagtttgcc ttacatcatt
     2461 ggcggacttg gcatcattat gatcgcaggc ggcttgatgt attacttcca atggggacgc
     2521 cactctgcag gcggtggtaa acatcggcgc cgttcacgaa cgaatacaga aagcgatgcc
     2581 accagcatct actgtccaca atgtggggca cgtgccaagc ctagtgacag gttctgtaga
     2641 acctgtggag caaggctaag gcacgacgaa gcataacgag gagagaccct aaaggcgaac
     2701 aaagcgcctt tggggtcttt ttatgtaagc atggaaaatc tgtctgttac agtcatctat
     2761 ttcttttatg ggcttgcctt tttcagcatg ggcttgttgg ttgccatgga aggagggcgc
     2821 tctacagatg c
//
LOCUS       DAOQJE010000472         2829 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_24417_length_2829_cov_5.564888, whole genome shotgun sequence.
ACCESSION   DAOQJE010000472 DAOQJE010000000
VERSION     DAOQJE010000472.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2829)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2829)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2829
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24417_length_2829_cov_5.564888"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..658)
                     /locus_tag="PLT08_11995"
     CDS             complement(<1..658)
                     /locus_tag="PLT08_11995"
                     /inference="COORDINATES: protein motif:HMM:NF013799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HNE05236.1"
                     /translation="MSKAILVLSDALRYDVAIANMGYLGHMVEYKKASLYKIVGELPS
                     MSRPMYETIHTGMPSSETGIVANSIVRLSEKPNIFKSVREAGMVTAAAAYYWYSELYN
                     RAPYNLIDDREVDDASLNIQHGRFYTTDEYPDIELFNTAGHLVRKYSPDYLLLHPMGM
                     DYHGETYGSDTKEYRNHAIYQDSKLAPLLVEWRERGYTIFVTGDHGINADGGHGGTTP
                     E"
     gene            complement(711..1772)
                     /locus_tag="PLT08_12000"
     CDS             complement(711..1772)
                     /locus_tag="PLT08_12000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNE05237.1"
                     /translation="MAFLSISNVSKQYGDATVVDNFNLDIEKGEFVSFLGPSGCGKTT
                     TLRMVAGFEIPTMGKIILDGADITNKAPNQRNVGMIFQSYALFPNMTVAQNIGFGLRV
                     RKEPEETVKKRVDEMINLINLEKHAHKYPYQLSGGQQQRVSLARALANRPRVLLLDEP
                     LSALDAKIRVSLRTEIRSIQKSLGITAIFVTHDQEEALSISDRIVVMNVGVIEQVGTP
                     FEIYNFPQTQFVANFVGSLNTAKAEVTDPANGLLTVDGVQFVTASDIKSRKKGDAIRV
                     SIRPERFSFADVQKKDNVLDCTIENITFLGSVVRIQVKIGESKFNMDTFNNPFLELPK
                     IGSKEQVTCSKEAVLVLDE"
     gene            complement(1792..2622)
                     /locus_tag="PLT08_12005"
     CDS             complement(1792..2622)
                     /locus_tag="PLT08_12005"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNE05238.1"
                     /translation="MKKRSSRNFPFWSWVWFILGALYFILPLYATLDFSLQMERDVIG
                     FKAYEKAFEDKDFIETFTYSNILAFATIAVSIILIVPTAYWIRLRVPEARRAVEFITL
                     LPFVVPGIILVFGLIRIYSSPLTIPFTDIVLMQPLTTYKFGTNVLIVAGYMVLAMPYM
                     YRSVDTGMRTVDVATLTEAAQSLGASWFTIITRIILPNIRSALLSGALLTFAIVVGEV
                     TLASFLGVDAFGPYLFLLGQHRAYEPAALSFVSFLLTWAAMGLIQLFTGGQGQTAGAH
                     "
     gene            complement(2619..>2829)
                     /locus_tag="PLT08_12010"
     CDS             complement(2619..>2829)
                     /locus_tag="PLT08_12010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="acriflavin resistance protein"
                     /protein_id="HNE05239.1"
                     /translation="NAFGAYATAYALTGGTLPIVTIQIGAQIRGDVLHNPGLGYAMAM
                     GMVIIMAISITGYTWLQKISERWLK"
BASE COUNT          789 a          699 c          664 g          677 t
ORIGIN      
        1 gctcgggcgt agtaccgcca tgccccccat cagcattgat gccatggtca cccgttacaa
       61 aaattgtgta tccacgctcg cgccattcca cgagaagagg cgcgagtttt gaatcttgat
      121 agatggcatg gttacgatac tccttagtat cagaaccata ggtctcgcca tgatagtcca
      181 tgcccatagg gtgaagcaaa agataatctg gcgaatattt gcggacgaga tgtcccgccg
      241 tgttgaacag ctcgatatct gggtattcgt cggtggtata gaaacgccca tgctgaatgt
      301 ttagggaagc atcatccact tccctatcgt caatgagatt gtaaggcgca cgattgtaca
      361 actcagaata ccaataatac gctgccgcgg cagtcaccat ccctgcttct cgtacagact
      421 taaaaatatt cggtttttca gaaagcctca caattgaatt cgcaacaatg ccagtttcgc
      481 tggaaggcat accggtatgg atggtctcat acatcgggcg agacatgctt ggcagttcac
      541 caacgatctt gtacaagctt gcttttttat attccaccat gtgacctaaa tagcccatgt
      601 tggcaatcgc tacgtcgtaa cgcagggcat cagaaagaac aaggatagct ttgctcataa
      661 aacctctata aagaagtagg tcacgctttg agcgtgacct acaatttttt ttattcgtcc
      721 agaaccagaa ccgcttcttt cgaacaggtg acctgttctt tcgaaccaat ctttggcagt
      781 tcaaggaacg gattattgaa ggtatccata ttgaatttgg attcacctat ttttacctga
      841 attcggacca cagaaccgag gaaggtaata ttctcaatcg tacagtcgag aacattatct
      901 ttcttttgaa catccgcaaa gctgaagcgt tccgggcgga tggaaacacg gatcgcatcc
      961 cccttcttgc gggatttgat atcagaggca gtcacaaatt ggacgccatc cacagtcaaa
     1021 agtccattcg cagggtctgt cacttctgct ttggcggtgt tgagggagcc aacgaagttc
     1081 gccacgaact gggtttgagg gaagttgtaa atctcaaagg gcgtaccaac ctgctcgatc
     1141 acacccacat tcataaccac gatacggtca gatatagaga gcgcttcttc ctggtcatgg
     1201 gttacgaaaa tggcggtaat gcccagtgac ttttgaatcg agcggatctc cgtacgcaat
     1261 gaaacgcgga ttttcgcatc caaagctgaa agcggctcgt ccaacaaaag cacacgcgga
     1321 cggttagcta atgcacgcgc caaggaaaca cgctgctgtt gcccgcccga aagctgatag
     1381 ggatatttgt gggcatgctt ttcgagattg atcaggttga tcatctcgtc cacacgcttt
     1441 tttacggttt cttccggctc tttgcgaact cgaagaccaa acccaatgtt ctgggcaacg
     1501 gtcatattcg ggaatagggc gtaggattgg aagatcatac ccacattgcg ttgattgggt
     1561 gccttattgg taatatccgc gccatccaga atgatcttcc ccatggttgg gatctcaaac
     1621 cctgccacca tgcgtaaggt tgtggtcttt ccacaaccag acggaccaag aaaagatacg
     1681 aattcgccct tctcgatatc gagattgaaa ttatccacca ccgtggcatc gccgtattgc
     1741 tttgaaacat ttgaaatgga aagaaatgcc attgccattc tcctcaaaag attagtgtgc
     1801 cccggcggtc tgaccttgtc cgccagtgaa taattggatc aaccccatcg ctgcccatgt
     1861 caacaggaag cttacaaatg aaagggcggc aggttcatac gcacgatgct gccccaacag
     1921 gaagagatat ggaccgaacg catccacgcc gaggaaggaa gcgagggtca cttcgcccac
     1981 tacaatggcg aaggttaaca aggcaccact aaggagcgcc gagcgaatgt tcgggagaat
     2041 gatccgtgtg atgatggtga accagctcgc gccgaggctt tgagccgctt ctgtcaaagt
     2101 tgctacatcc acagtgcgca tgcccgtatc caccgaacgg tacatgtatg gcatggcaag
     2161 caccatatag ccagccacga tcaacacgtt ggtgccgaac ttataggtcg tgagaggttg
     2221 catcaaaaca atatcggtaa atggaatcgt caaaggtgaa ctgtaaattc ggatcaaccc
     2281 aaacaccaag ataattccgg gcaccacaaa cggcaacaag gtgatgaatt ccacagcacg
     2341 gcgcgcttct ggaacacgca agcgaatcca gtatgcagtt gggacgatca gaataatact
     2401 gacagcaatg gttgcaaacg ccaaaatgtt ggaatatgtg aaagtctcaa tgaagtcttt
     2461 atcttcaaag gctttctcat acgctttgaa accaatgaca tcgcgctcca tttgaaggga
     2521 aaaatcaaga gtggcatata ggggcaaaat aaaatacagt gcgcctaaaa taaaccaaac
     2581 ccagctccaa aaaggaaagt ttcgtgatga tcgttttttc atttcaacca cctttcagat
     2641 attttctgta gccaggtata ccccgtgatg gaaatcgcca tgatgataac catacccatt
     2701 gccatggcgt accccaaacc gggattatga agaacgtcgc cgcggatctg cgccccgatc
     2761 tggatcgtca caattggcag ggtgccaccc gtcaatgcat aggctgtggc atacgcacca
     2821 aacgcattt
//
LOCUS       DAOQJE010000473         2827 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_24451_length_2827_cov_5.578283, whole genome shotgun sequence.
ACCESSION   DAOQJE010000473 DAOQJE010000000
VERSION     DAOQJE010000473.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2827)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2827)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2827
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24451_length_2827_cov_5.578283"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..304)
                     /locus_tag="PLT08_12015"
     CDS             complement(<1..304)
                     /locus_tag="PLT08_12015"
                     /inference="COORDINATES: protein motif:HMM:NF021539.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2270 domain-containing protein"
                     /protein_id="HNE05240.1"
                     /translation="MPRKKEAPPQQPLQEEESVWTYRGYKLKSSEFVTAMVHLFRAEV
                     QRANVWRQRLDTTTNWAVVATGATLSISFSQPNVHHGVIILNTLLVTWFLFIEARRY"
     gene            complement(509..847)
                     /locus_tag="PLT08_12020"
     CDS             complement(509..847)
                     /locus_tag="PLT08_12020"
                     /inference="COORDINATES: protein motif:HMM:NF012790.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HNE05241.1"
                     /translation="MSLIEMLFGKPIPEITPHELNEKLKDKRKFILIDVRQPEEFKSG
                     HVPGAKLIPLGELSRRLDELPKDKEIACICASGSRSSSAARMLAKQGYNVFNVRGGMY
                     LYSRTQFKGK"
     gene            complement(970..2247)
                     /locus_tag="PLT08_12025"
     CDS             complement(970..2247)
                     /locus_tag="PLT08_12025"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013227.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HNE05242.1"
                     /translation="MSELAILGGSKTRTEDYPQWPVWDQRDIDAVTEVVKSGRWGGFP
                     YPGPKTAELAKKFAELQGGGYAVPMINGTITMEVALRAAGIGWGDEVIVPAYTFQATA
                     SAPMGAGAIPVIVDVDPNTYCLDPKAVEKAITSKTKAIIPVHLGHQMADMDALMALAE
                     KYNLIVIEDCAHAHGAKWNGMGAGTIGHFGSFSLQSSKTLTSGEGGILLCKSPEHAAL
                     AASIIDCGRPHALGGGEEDSNGLSIQGGNFRMSELQSALALIGIERFPAQAKQREEMA
                     AYMDEALSEVKGVRVLKRDPRHTTRSFYRYVFAIDPAEFGIEHDVLCAALDAEGVGCW
                     TGYEAMHNYELFQPQKSKLAVPNAFPQYFDFKKMNLPEATRACEHEAVWLDEAIFRAG
                     PKGVDDAVAAIKKIQKNAQALSVAADELRKQFS"
     gene            complement(2282..>2827)
                     /locus_tag="PLT08_12030"
     CDS             complement(2282..>2827)
                     /locus_tag="PLT08_12030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE05243.1"
                     /translation="SPFGEFGGAHEILARGLQMVNYAWIKRIPPTALTKIYIDAVGAK
                     DEMDLMEGLSNESYHLFPYIVLQIVDAARAGDHAACATLKWAGEELGMLAVSVARQIE
                     MDHEEVEVIQSGSVFQAGELITNSMKTTVLAHCPKAKLIRLEGPPVVGAVILGMEQAG
                     FDGYAVRDAMVKSAKEIMKDV"
BASE COUNT          741 a          739 c          576 g          771 t
ORIGIN      
        1 gataccggcg cgcttctata aataagaacc atgtaaccaa tagtgtattt aaaataataa
       61 cgccgtgatg tacattgggc tgactgaatg aaatggaaag cgtggcacct gtagcgacta
      121 ctgcccaatt ggtggtcgta tccaagcgct gacgccaaac atttgcacgc tgcacttctg
      181 cacggaaaag atgtaccatg gcagtgacaa attcactgct tttgagtttg tacccgcgat
      241 acgtccaaac agactcttct tcttgcagag gttgttgagg cggagcttct ttttttctag
      301 gcatgaggtt tatccgatct ttaagaatta attttatttc ttgaaaaatc tcaggtcaat
      361 aaacgagttt acacccacta aagagagtac ttcatggggc tttcattacg gttgtcactt
      421 aagccgatgg gctgtctccc tctttataac tcaatgcctt aaataaaaga cagcccagta
      481 aattcactgg gctgttgaac aaatacagtt actttccttt aaattgggtg cgagaataaa
      541 gatacatacc cccgcgaaca ttaaagacgt tgtatccctg tttggcaagc atgcgtgcag
      601 cagaactgct tcggctccct gaagcacaga tacaagcaat ctccttatct tttggcagtt
      661 catcgaggcg gcggctcaac tcacccagcg gaatcaactt tgcgcctggg acatgcccgc
      721 ttttgaattc ctctggctgg cgcacatcaa taagaataaa ctttcgttta tcttttaatt
      781 tttcattcag ttcatgtggg gtaatttctg gaattggctt tccaaacaac atttcgatca
      841 aactcatact ggctatctcc aaattttgat atatgttttg atgcacatcc tcaaaaaaag
      901 atacaaataa gagcgaaaca cagtttgcat ttcgctcttg ggaattacta aacatgaaaa
      961 ttttctaaat tagctaaatt gttttcgcaa ttcatctgcg gcaacgctta acgcttgcgc
     1021 atttttctga atcttcttga tcgccgccac ggcatcatcc acgcctttgg gtcccgcacg
     1081 gaagatcgcc tcatcgagcc agactgcttc atgttcgcaa gcgcgcgtgg cttcgggcaa
     1141 attcattttc ttgaaatcaa aatattgggg gaatgcattc ggcacagcta gcttggattt
     1201 ttgcggctga aataattcat agttatgcat agcttcataa cccgtccaac aaccaacacc
     1261 ttcggcatct aaggcagcac aaagcacatc atgttcgata ccaaattcag ccgggtcaat
     1321 cgcgaagaca taacggtaaa acgaacgagt tgtatgacgt gggtcgcgtt ttaagacacg
     1381 cacacctttc acttcactga gggcttcatc catataggca gccatttctt cgcgttgttt
     1441 tgcttgcgca gggaaacgtt caatgccgat caacgccaaa gcagattgca gctcgctcat
     1501 gcggaagttc ccaccttgaa tggatagccc atttgagtct tcttccccgc cgcccaatgc
     1561 atgtggacga ccacaatcaa taatggatgc agccaatgca gcatgctcgg gagatttaca
     1621 caaaagaatc ccaccttcgc cagatgttaa tgtcttcgaa gattgcaaag agaacgagcc
     1681 aaaatgaccg atggtgcctg cccccatgcc attccatttt gcgccgtgcg catgagcaca
     1741 atcttcaata acgatcaagt tatatttttc cgccaatgcc ataagcgcgt ccatatctgc
     1801 catttgatga ccgagatgca cagggatgat cgctttggtc ttggatgtga tggctttctc
     1861 tacagctttc gggtccaaac aataagtatt cgggtccaca tccacaatga cgggaatggc
     1921 acccgcaccc atcggtgcag aggcggttgc ctgaaaggta tatgcaggta cgatcacttc
     1981 atctccccaa ccaatgccgg cggcacgcaa tgccacttcc atggtgatgg taccgttgat
     2041 catcggtaca gcgtatccgc ccccttgtaa ctcagcaaat ttcttcgcca gttccgctgt
     2101 cttcggtccc gggtacggaa acccgcccca acgcccagat ttcactactt cggtcaccgc
     2161 atcaatatcc cgttgatccc atactggcca ctgaggatag tcttctgttc gggtttttga
     2221 gccaccgaga attgctaatt cagacatgga tactccttca gactttcaaa tgttatttac
     2281 actacacgtc tttcattatt tcttttgcgc tcttcaccat cgcatcgcgc acggcataac
     2341 cgtcaaagcc ggcttgctcc attcccaaaa ttactgcgcc caccactggc ggaccctcaa
     2401 gccgaatcaa ctttgctttg gggcaatgtg ccaacacagt tgttttcatt gaatttgtaa
     2461 tcaattcgcc tgcttgaaaa acactgcctg attgaataac ttctacctct tcatggtcca
     2521 tttcaatttg tcgtgccacc gaaactgcca acatgcccaa ctcttccccc gcccatttca
     2581 gggtcgcaca agcagcatgg tcacctgctc gtgccgcatc tacgatctgc aaaacaatgt
     2641 atggaaaaag atgatagctt tcgttcgaga gcccttccat caaatccatt tcgtctttag
     2701 cacctacagc gtcgatataa atttttgtca acgccgtggg cggaatacgt ttgatccacg
     2761 cataattcac catttgcaaa ccgcgtgcca aaatttcatg tgccccgcca aactcaccga
     2821 acggaga
//
LOCUS       DAOQJE010000474         2824 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_24492_length_2824_cov_8.054171, whole genome shotgun sequence.
ACCESSION   DAOQJE010000474 DAOQJE010000000
VERSION     DAOQJE010000474.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2824)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2824)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2824
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24492_length_2824_cov_8.054171"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1295)
                     /gene="atpA"
                     /locus_tag="PLT08_12035"
     CDS             complement(<1..1295)
                     /gene="atpA"
                     /locus_tag="PLT08_12035"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HNE05244.1"
                     /translation="MSDLIKQITGDLQKQIEGFKPEFNVSEAGVVLEAGDGIARVHGL
                     ANIKAQELVQFANGVIGVAFNLEKDNVGVIVMGAYEGITEGMSVKGLGRIASVPVGDA
                     MIGRVVNALGEPIDGKGPIATTGFRPIERIAPGVVERQDVDTPVQTGIKSIDSMIPIG
                     RGQRELIIGDRQTGKTAVAIDAIINQKGKGVTCIYVAVGQKKAAIARTLGILEKYGAM
                     DHTIIVVASADESAALQYIAPYAGTAIGEEFMETGRDALIIYDDLSKHAWAYRQVSLL
                     LRRPPGREAYPGDVFYLHSRLLERSARLANQYVVTKKDFSADLADAKDGVDGKVFTGP
                     LSKHEAEEAAKAMGEGFKAVKVKGTGGSLTSLPIIETLLGDVSAYIPTNVISITDGQI
                     YLESNLFNAGIRPAVNVGISVSRVGGDAQTKAMKQVAGR"
     gene            1539..2675
                     /locus_tag="PLT08_12040"
     CDS             1539..2675
                     /locus_tag="PLT08_12040"
                     /inference="COORDINATES: protein motif:HMM:NF019121.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE05245.1"
                     /translation="MNTRRPSKRQWKIAVLANIKDDNQPKPDNVPPDAFADFDHIETI
                     DSLRAALETDGHTTVFVHADRELPYSLRKVKPDICFNIAEGLGGDAREAQVPALLEML
                     AIPYTGSRVLANGISLDKTLTKRIWRDRRLPVAPFQEFQFGDEPLRPELKFPLFVKPA
                     REGTGMGVDMKAIVNNETELRERAEYIINTYQQPALVETFLPGREFTVGILGRADAKL
                     YSRHPEWYEKDGFHRFPVLELDTGNTEGPWVYTNEAKSKDVGPEGNEGFFCPANIDPE
                     LEKKLKHLAFRAHNILNVLDVSRTDIRLDEEGNPRLIEINTLPGLTPNYSDLCLQAAA
                     EGISHTDLVLDILYLAAGRWGLLEPREISTESGKTGPLSYRLGR"
BASE COUNT          732 a          766 c          673 g          653 t
ORIGIN      
        1 aaacgacctg caacctgctt catcgctttg gtctgagcat caccacctac gcgggaaacg
       61 gagataccta cgttcaccgc ggggcgaata ccggcattga agaggttgct ttcgagataa
      121 atctgaccat cggtgatgga gatcacgttg gtcgggatat aagcagaaac gtcaccgagc
      181 aaggtttcga tgatgggcaa cgaagtgagt gaaccaccgg ttccctttac tttgacagct
      241 ttgaagccct cacccattgc cttggcagct tcttccgctt catgcttgga aagaggaccc
      301 gtgaacactt tgccatccac accatctttg gcgtcggcga ggtcagcgct gaaatctttc
      361 ttggtcacca catattgatt ggcgaggcgc gcggagcgtt ccaacaagcg ggagtggagg
      421 tagaacacat cgccggggta ggcttcgcgt ccaggaggac ggcggagaag caaggaaact
      481 tggcggtacg cccaagcgtg cttggaaaga tcgtcataga taatgagagc atcgcgaccg
      541 gtttccatga actcttcacc gatggcggta cccgcataag gagcaatgta ttgaagcgca
      601 gcagattcat ctgcagaagc caccacgata atggtgtggt ccatcgcgcc atatttttcg
      661 agaataccaa gggtacgagc aatggcagct ttcttctgac ccactgccac atagatacaa
      721 gtaacaccct tgcccttctg gttgatgatc gcatcgatag caacagcggt cttaccagtc
      781 tggcggtcgc cgatgatcaa ttcgcgttga ccgcgaccga tcgggatcat cgagtcgatg
      841 gatttgatac cggtctgaac cggggtatcc acatcctgac gttccaccac gccgggagca
      901 atgcgctcaa ttgggcggaa gcccgtggtt gcgataggac ccttaccgtc aatgggctcg
      961 cctaaggcgt tgaccacacg tccgatcatg gcatccccta ccggtacaga agcgatgcgt
     1021 cccaaaccct taacagacat gccttcggta ataccttcgt acgcacccat cacgatcaca
     1081 ccaacgttgt ccttttccaa gttgaacgca acgccgatca caccgttggc aaattgtacg
     1141 agttcctgtg ccttgatatt cgcaagacca tgaacacgag caatgccatc acccgcttca
     1201 agaacaacac cagcttcgct gacgttgaat tcgggcttga atccctcaat ttgtttctgc
     1261 agatcaccag tgatctgctt gatcaagtct gacattcggt agcctccacc aaaaaaattc
     1321 gttttacgta caacaacccg gataaggggc ttatccgggc aaaatattca actcttggca
     1381 cttcgtgagt aagcgcacaa gtgggctaat gatacctgaa aggtggacga ttgtctagtt
     1441 actccccaaa cgggtgaaga aatgcacttt ccccttgtca tccatacatc ttatactata
     1501 ctacaagggt gaaatttaca ggagcgaccc acacatagat gaatactcgc cgtccgtcga
     1561 aacgccaatg gaagatcgca gtacttgcca atattaagga tgacaaccag cccaaaccgg
     1621 ataatgttcc cccggacgct ttcgctgact ttgaccatat cgaaacgatt gactcgcttc
     1681 gtgccgcgct cgaaacggat ggtcacacta ctgtctttgt ccacgcggat agagaactcc
     1741 cctacagcct ccgcaaggtc aagccagata tctgcttcaa tattgccgaa ggtcttggag
     1801 gtgacgcccg tgaagcacaa gtgccagctt tactggaaat gctcgccatt ccatacaccg
     1861 gttcccgtgt gcttgccaat ggcatctcac ttgataaaac actgaccaaa cgtatctggc
     1921 gtgaccgtcg tctgcctgtg gctccctttc aagaattcca atttggcgac gaacccttac
     1981 gccccgagct gaaattcccg ctcttcgtca aacctgcccg cgaagggaca ggcatgggcg
     2041 tagacatgaa agccatcgtc aataacgaaa cagaattgcg cgaacgtgca gaatacatca
     2101 tcaacaccta tcagcaacct gctctcgttg aaaccttttt gcccgggcgc gaattcacag
     2161 tgggcatctt gggtcgtgcc gatgccaagc tatattctcg tcaccccgaa tggtatgaaa
     2221 aagatggctt ccaccgcttc cctgttttgg aattagacac aggcaatacg gaaggtccat
     2281 gggtttatac aaatgaagct aaatcaaaag atgttggtcc tgaaggcaac gaagggtttt
     2341 tctgcccagc caacattgac ccagaacttg agaaaaaatt aaagcacctt gcctttcgtg
     2401 ctcataacat tctcaatgtc cttgatgtct ctcgcacaga cattcggttg gacgaagaag
     2461 gcaatccacg cctgattgag atcaacacct tgccagggct tacccctaac tatagtgacc
     2521 tctgcttaca agctgcggca gagggcatca gccatacaga cttagtgctg gatattctct
     2581 atctcgctgc tgggcgttgg gggcttcttg aacctcgtga gatttcaact gaaagcggaa
     2641 aaacaggtcc cctatcgtat cgtttagggc gataagtccg cataaaaaaa gagcgctagg
     2701 gtagcctagc gctctttttt taatggcaaa gactattaat cgtcgtctcc gccttcgcca
     2761 ccttcaaacc caccgctatg acaagagtca cagctagact cgaagacaga tgaggggtga
     2821 catt
//
LOCUS       DAOQJE010000475         2823 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_24511_length_2823_cov_4.455202, whole genome shotgun sequence.
ACCESSION   DAOQJE010000475 DAOQJE010000000
VERSION     DAOQJE010000475.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2823)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2823)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2823
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24511_length_2823_cov_4.455202"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(212..574)
                     /locus_tag="PLT08_12045"
     CDS             complement(212..574)
                     /locus_tag="PLT08_12045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE05246.1"
                     /translation="MQSPEKPSRLGTISFILAILVVVVWCIYLIIFAATTEGGLNFGM
                     DGETAGYAVVLGGSVIMMVLTILLTLSGIVTGALALRNKDSKRGLAIAGLVLNFLCFA
                     PYCLLLALMAFSAIPSGN"
     gene            complement(613..999)
                     /locus_tag="PLT08_12050"
     CDS             complement(613..999)
                     /locus_tag="PLT08_12050"
                     /inference="COORDINATES: protein motif:HMM:NF013222.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MGMT family protein"
                     /protein_id="HNE05247.1"
                     /translation="MSFSSPPNPQAYYEQVWAIARLIPHGKVATYGQIAKMIPPPNGV
                     ELEAYVAFGPRWVGGAMANCPDDVPWQRVINSQGKISERPGAERQRPLLEAEGVIFDA
                     KDRVDLKKFGWSGKDESDVPQQPSLF"
     gene            complement(1036..1395)
                     /locus_tag="PLT08_12055"
     CDS             complement(1036..1395)
                     /locus_tag="PLT08_12055"
                     /inference="COORDINATES: protein motif:HMM:NF013222.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MGMT family protein"
                     /protein_id="HNE05248.1"
                     /translation="MPELPTLPDPQAFYAQVWNIVVRIPRGKVASYGQIAKMIRPPAG
                     VDAETYAEFGALWVSNAVAASPNEVPWQRVVNSKGQITERDGLEAKRHKLMLEDEGVL
                     FHDRGRIDMRKYGWNGK"
     gene            complement(1416..>2823)
                     /locus_tag="PLT08_12060"
     CDS             complement(1416..>2823)
                     /locus_tag="PLT08_12060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE05249.1"
                     /translation="YESLPTTNHQLSTVYWLLLTVLWLFLSGIGGYAFQNWDHNWRNV
                     VFRDLMNFDWPVYYAQPESGPVKMLVYYVGFWLPSALIAKFTNWQVANFALFVWSLLG
                     LLLVTHHLGNFFKTSNLKATLLLIFFSGLDVLGTLFFAKGYPTLLPPITHLETWAGNL
                     QYSSFTTQLFWVFNQSIPAWLCIMLILILSREAAKDLSTVAPETLRSAQSDTRSEIIL
                     LWSLCFFFAPLASVGLLPYILIELIKQTDFKYPFKHLNPAVVLSSIIIFLLSAFFFTA
                     NTAAQSRGFQSLPPIKFLAFFLLEGGFLWLVLAPSKYKDPRWIITGILLALIPFIQLG
                     SDRDFVMRASIAPLFYLMLLTGETIFKKSAHHVSFFTHYSILALLVLGAFTPLYEINR
                     SIYRTFDYYFIQQDCGCEFAADPITHIVHPDVPEKEHPGSITADALPTLQFLTTELSQ
                     NFIANVRQTFFYKYLAKR"
BASE COUNT          765 a          572 c          819 g          667 t
ORIGIN      
        1 aggggttgac tgtgtcttga aaaaattcaa aacgtaatac accaggttca agacgagtct
       61 tttgtgcatt ttccagcgtg acagctttat aggcgtcaat gccttcaggt ttaatgtaat
      121 gatggacttg aaagatatac atagtctgcc tccgttttca aaatgtactt gaaagtcgta
      181 gagacaaact atgcaatatc attgcaagag gttaatttcc tgaagggatg gcgctgaatg
      241 ccatgagtgc caataaaaga caatagggag caaagcatag aaaattaagg accaacccgg
      301 caatggcgag accgcgcttg gagtctttgt tcctcaaggc aagggcgcca gtaacgattc
      361 cagaaagggt tagcaagatg gtaagaacca tcatgattac actgccaccc aaaacaacgg
      421 catacccggc agtttcgcca tccatgccga agttgagtcc gccctcggtg gttgcggcga
      481 agataatcag gtaaatacac caaacgacca cgaccaaaat agcaagaata aaagaaatgg
      541 ttccaaggcg ggatggtttt tcaggggatt gcataggtgt ctccttatta aagttagtta
      601 tcagattata ggttaaaaca aagatggttg ctgtggcaca tcgctttcgt ctttgccgct
      661 ccagccgaac tttttcaaat ccactctgtc tttggcatcg aagataaccc cttccgcttc
      721 caataacgga cgttgcctct ccgcgccggg gcgttcgctg atcttgcctt gcgagttgat
      781 gacgcgctgc caaggcacat cgtctgggca gtttgccatg gcaccaccca cccaacgcgg
      841 accaaatgca acatacgctt caagttccac gccattaggc ggtggaatca ttttggcgat
      901 ctgcccgtag gtagcaactt ttccatgtgg gatgaggcga gcgatcgccc aaacttgctc
      961 ataataagct tgcgggttgg gaggagagga gaaggacata agagtttctt tctcggttta
     1021 tggtttaccg ctttattatt ttccgttcca gccgtatttt cgcatatcga ttcgtccacg
     1081 gtcatggaag aggacacctt catcctcaag catcagttta tgacgcttcg cctctaaccc
     1141 gtcccgttcg gtgatctgcc cctttgagtt gaccacacgc tgccacggaa cttcattcgg
     1201 gctggcagcg acggcgttgc tgacccatag tgcgccaaac tctgcatacg tttctgcatc
     1261 cacaccggcg ggcggacgta tcatcttggc gatctgccca tacgaagcga cttttccgcg
     1321 tgggatgcgg acgacaatgt tccatacttg agcgtagaaa gcttgagggt ctgggagggt
     1381 tggtagttcg ggcataagaa tatccttttt gatggttagc gtttagccaa gtatttatag
     1441 aagaatgtct gtcgaacatt ggcgatgaag ttttgtgaga gttccgttgt cagaaattgc
     1501 agagtgggca gtgcatcggc agtgatggag ccggggtgtt ccttctctgg cacatcgggg
     1561 tggacgatat gagtgatcgg gtccgctgca aattcacagc cgcaatcttg ttgaatgaaa
     1621 tagtaatcaa aggtgcggta tatggagcgg ttgatttcgt aaagtggagt aaaggcacca
     1681 agaactaaga gagcaaggat tgagtaatga gtgaagaatg aaacgtgatg agcagatttt
     1741 ttgaagatgg tttcgcctgt taagagcatg aggtagaaaa gcggggcaat tgaggcgcgc
     1801 atgacaaagt cgcggtcgct gccgagttgg atgaaaggga tgagggcaag cagaatgccg
     1861 gtaatgatcc aacgcgggtc tttatatttg gatggagcaa gtaccagcca aagaaatccg
     1921 ccttcaagca ggaagaaggc gagaaattta atgggtggta aggattggaa tccgcgggat
     1981 tgggcggctg tgtttgcggt gaagaagaaa gcggaaagaa gaaagatgat gatagacgag
     2041 agtacgacgg ctgggttgag atgtttgaag gggtatttga agtcggtttg tttgatgagt
     2101 tcaatgagga tgtatgggag aagtcctacc gaggcgagcg gggcgaagaa gaagcagaga
     2161 gaccagagaa ggatgatttc gcttcgcgtg tcactctgag cggagcgaag agtctctggc
     2221 gcaactgtgg agagatcctt cgccgcttcg cggctcagga tgagaattaa cataatacat
     2281 aaccatgcag gaatggattg gttgaaaacc caaaacaact gcgtagtgaa cgatgagtat
     2341 tgcaaattcc ctgcccaagt ctcgagatga gtgatgggcg gcaggagggt gggatagcct
     2401 ttagcaaaga aaagcgtgcc aagaacgtca agtccgctga agaagatgag gagtagggtg
     2461 gctttgagat tggatgtttt gaaaaagtta ccgaggtgat gagtgacgag taacaagcca
     2521 aggagtgacc aaacgaagag ggcgaagttg gcaacttgcc agttggtaaa tttcgcgatc
     2581 aacgcggaag gcagccagaa gccgacgtaa tagacaagca tcttgaccgg tccgctttcg
     2641 ggttgagcgt agtagacggg ccagtcgaag ttcatcaagt cgcggaagac aacattgcgc
     2701 cagttgtggt cccagttttg gaaggcgtaa ccgccgatgc cggaaaggaa gagccagagg
     2761 acagtgagca ggagccagta gacggttgat agttggtggt tggtggttgg tagtgattcg
     2821 taa
//
LOCUS       DAOQJE010000476         2822 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_24520_length_2822_cov_5.490784, whole genome shotgun sequence.
ACCESSION   DAOQJE010000476 DAOQJE010000000
VERSION     DAOQJE010000476.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2822)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2822)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2822
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24520_length_2822_cov_5.490784"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..365)
                     /locus_tag="PLT08_12065"
     CDS             complement(<1..365)
                     /locus_tag="PLT08_12065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE05250.1"
                     /translation="MRLSTKLILSFMLISIFSTGIIVVSSRILASREFERFVGDRYTA
                     DLADDLARYYSRNQTWDGVEQEFQHGDHDHHSLYFSIADPQGNIVVAGNEYNVGDPCS
                     QEEFEEGYPIQSNNKTVGVL"
     gene            complement(376..1059)
                     /locus_tag="PLT08_12070"
     CDS             complement(376..1059)
                     /locus_tag="PLT08_12070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HNE05251.1"
                     /translation="MPQKILIVDDERRMVSLLQSYLEQEGYHVLTAYNGREALEVARR
                     ENPDLVVLDIMMPEMNGYEFMNARRAESDTPIIMLTAKVEDEDKIIGLELGADDYVTK
                     PFKPRELMARVRNVLRRAGKNEAKGQTLKVLDITLNRETREVSIGDRHADLTPSEFDL
                     LAALMTAPGKVFSRLDLLDVIQGVRYEGYERTIDTHVKNLRVKVEDDPRKPSHIETVY
                     GVGYRLRKQ"
     gene            complement(1167..1610)
                     /locus_tag="PLT08_12075"
     CDS             complement(1167..1610)
                     /locus_tag="PLT08_12075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE05252.1"
                     /translation="MKTKKWLWWTLTILLTLVVLTGVAIAGFRFGAMHSATLTAEGTS
                     AFAFHHGHDFSSNMTHEGFGMMRGSDPGHGFGGRGGFGFFGPLFGLIQLAVFGGLVWL
                     AFSLIKRSGWRLVNVNNSQAAAPAPDVAPEAAEVQVESEEKKDEA"
     gene            complement(1727..>2822)
                     /locus_tag="PLT08_12080"
     CDS             complement(1727..>2822)
                     /locus_tag="PLT08_12080"
                     /inference="COORDINATES: protein motif:HMM:NF014717.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HNE05253.1"
                     /translation="TLFTLVLGGAGFIAVYNLWASFDKTIEDIQGYFLADINISFDKG
                     YRYDKVASIATSIPGVEGAEGWLEWTGTLEMNDDEAGTQILFVAPPSNSKLIDPIITS
                     GRWLQPGDENAIVIGNHLLQIFPDLKVGDWLNIDIDGKKTKWQIVGTYSITGNVSPPL
                     LYVNYEYLSVLVGEPEMAYSLRIVTSQHDSATQNRINDQIQAAFEERGIQISSTQLGA
                     DFIRDQKAQTDVLVYFMLVMASLIAFVGGLGLMGTMSINVLERTREIGVMRAIGASNG
                     DIQAIVITEGMVIGILSWAISIFISIPITNVLCYGVGMAILTAPMPAIYGMTGILAWL
                     IFTLVLGTLASALPARRASKLTVRDTLAYE"
BASE COUNT          737 a          689 c          664 g          732 t
ORIGIN      
        1 agcacaccga ctgttttgtt gttcgattga atggggtagc cctcttcaaa ctcttcctga
       61 ctgcacggat caccaacatt gtactcattg cctgcaacga ctatattgcc ttgcgggtct
      121 gcaattgaaa aataaagcga atgatgatcg tggtcaccat gttgaaattc ttgttccaca
      181 ccatcccagg tttgattacg gctgtaatac cgagccaggt cgtctgccag gtcagcggtg
      241 tagcgatcgc caacaaagcg ttcgaattcg cggctggcaa gaatgcgcga agaaacgaca
      301 atgatgccgg tactaaatat gctgataagc atgaacgaaa ggatgagttt ggttgaaaga
      361 cgcatgattt tgaaattact gttttctaag gcgataccct acaccataca cagtttctat
      421 atgagaaggt tttcgtgggt catcttctac tttaactctt aagttcttga cgtgagtatc
      481 aatcgtgcgc tcatatcctt cgtagcgcac accttgaatc acatcgagca ggtcaaggcg
      541 cgaaaaaact tttccggggg cggtcatgag cgctgcaagc aggtcaaatt cagaaggggt
      601 taagtctgcg tggcggtcac caatgctgac ttcgcgtgtc tcacggttga gtgtgatatc
      661 gaggactttc aacgtttgcc ctttggcttc gttcttgccg gcacggcgca atacattgcg
      721 aacacgagcc atcaactcac gaggcttgaa aggtttggtc acataatcat cggcaccaag
      781 ttcgagtcca atgattttat cttcatcttc cactttcgcc gtcagcataa taataggcgt
      841 gtcgctttcg gcgcggcgcg cattcataaa ctcgtagccg ttcatttccg gcatcataat
      901 atcaagcaca acgaggtctg ggttttcacg gcgggctact tccaaggctt cacgcccgtt
      961 atacgccgtt aatacgtgat acccctcttg ctcaagataa ctttgcaaga gagaaaccat
     1021 tcgtcgttca tcgtctacga taagaatttt ctgaggcatg aatcgattat cccataaacc
     1081 ctgtgaagaa attgtgtgaa atttaataga acaagagcac ccgcctcatt ccaaggcggg
     1141 tgctgctgcg gcgttagaac agtggattac gcctcatcct ttttctcttc gctttcaacc
     1201 tgcacttcag cagcttccgg cgctacgtct ggtgctgggg cagcagcttg tgagttgttg
     1261 acattcacaa gtcgccatcc gctgcgtttg atgaggctaa atgccaacca aaccaagccg
     1321 ccgaacaccg cgagttgaat cagcccaaat aacggaccga aaaagccaaa tccgccacgt
     1381 ccgccgaaac catgtccggg atcagagccg cgcatcatgc cgaagccttc gtgcgtcata
     1441 ttgctgctga aatcatgtcc gtgatggaac gcgaaagcgg aagttccttc tgcggtgagc
     1501 gtggcgctat gcatagcacc aaaacggaag cctgcaattg caacgcctgt caacaccaca
     1561 agagtcagaa ggatggtgag agtccaccaa agccattttt ttgttttcat gtttacctcc
     1621 atttgtaaac ttgactctat tttttcacac ctgtattaca aagcaatgga ggaaatgtgt
     1681 gaaagttgtg tgttcgcaga gattgtaaat aacgtaccta tagactttat tcgtaagcca
     1741 gcgtatctct cacagtcaac tttgaggcgc ggcgtgcagg taacgcactg gcaagagtgc
     1801 ccaacacaag agtgaagatc agccatgcaa ggatgccggt catgccgtaa atggcaggca
     1861 tgggagcggt gagaattgcc atgccaaccc catagcacaa gacatttgtg atgggaattg
     1921 aaatgaagat gctaattgcc caactcagga tgccgattac cattccttct gtgatgacaa
     1981 tcgcctgaat atcgccgttg cttgcgccaa tcgcgcgcat cacaccgatt tcacgagtgc
     2041 gttccaacac attgatgctc atggtgccca tcaagcctaa accaccgacg aaagcaatca
     2101 atgatgccat gaccagcatg aaatatacga gaacgtctgt ctgtgctttc tggtcgcgga
     2161 tgaaatctgc gcctaattgc gtggagctga tctgaatgcc gcgttcttcg aatgccgctt
     2221 gaatctggtc attgatacgg ttttgagtgg cagaatcatg ttgacttgtg acgatacgga
     2281 gggagtatgc catttcaggt tcgccgacca aaacgcttaa gtactcataa ttcacatata
     2341 acaatggcgg gctgacgttg ccagtgatgc tataagtgcc gacaatttgc cattttgtct
     2401 ttttaccgtc aatatcaatg ttgagccaat caccaacttt caggtctgga aagatttgca
     2461 agagatgatt gccgatcaca atcgcattct catcaccggg ttgcaaccag cgaccagaag
     2521 tgataatggg gtcgatcaat ttcgagttag aaggaggcgc cacaaataat atttgagttc
     2581 cggcttcgtc gtcattcatt tcaagggtgc cggtccattc cagccatccc tctgcacctt
     2641 ctactccagg aatgcttgtg gcaatgctgg cgaccttatc gtatcgatag cctttatcaa
     2701 acgaaatgtt gatgtcagcc agaaagtacc cttgaatatc ttcgattgtt ttatcaaagg
     2761 atgcccacaa gttgtaaaca gcaatgaatc cagccccgcc aagtactagt gtgaagaggg
     2821 tt
//
LOCUS       DAOQJE010000477         2814 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_24647_length_2814_cov_5.845959, whole genome shotgun sequence.
ACCESSION   DAOQJE010000477 DAOQJE010000000
VERSION     DAOQJE010000477.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2814)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2814)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2814
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24647_length_2814_cov_5.845959"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            39..938
                     /locus_tag="PLT08_12085"
     CDS             39..938
                     /locus_tag="PLT08_12085"
                     /inference="COORDINATES: protein motif:HMM:NF012798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HNE05254.1"
                     /translation="MTKNSAPAHISAKTLLPAAINGWEMFLLDQGRSPNTVKAFLSDV
                     RLLTQFLPPDQAIGTVTTDDLNRFFEWMEKGRGVACSPKTLSRRITSVKSFFRWLHQY
                     GTLSIDPAEKVLQRSAISPLPQVLTDEEYEAVLLAADRQRRAKKPDARPYTLLSLLLQ
                     TAIKKSECLGIHVNHIELAGKNGPFLFVRYASPANRYKERKIELTDDWVEAYNEYLTQ
                     YSPVDQAFPWSPRRLEYLLEDISEEAGLEKHLSFDMCRWTSALRDYQNGIEPDKIRQK
                     LGVSKIQWRELFIKLKQLNGEVK"
     gene            complement(958..2421)
                     /gene="tilS"
                     /locus_tag="PLT08_12090"
     CDS             complement(958..2421)
                     /gene="tilS"
                     /locus_tag="PLT08_12090"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR02432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HNE05255.1"
                     /translation="MSSNVETIMVEECGLVKDRPVIVGVSGGPDSLCLMETLRRAGYH
                     LIIGYFDHKLRPESALDARMVEKAAVRLGLLCILGGMDVRSYAEEKKLSIEEAARTLR
                     YRFLFELARKYKAQAVAVGHTADDQVETVLMHFLRGSGISGLKGMSYRSVIKSFDPDI
                     PVVRPLLDMWREETVLFCAVNGLRPSYDSSNESLNFQRNRIRHLLIPSLETYNPKFRE
                     TVLRMSHSLKGDYAFVMETLEHAWGNVVVSANDEIITFDVLRLSSMPIGLQRNLIRHA
                     MQTLLPGVDIGYQTLKRASDFINASSATVPRLDLKAGLRMFREAGLIHICAPYAELPF
                     DRWPQMVDDKSVPLRLGGQASLAGGWKVICESWRLPTLAREQAEENEDQFQVWLDADE
                     LPEEFLLRARRQGDHFSPLGMGGHSQKLSDFFVNEKLPHRARDRWPLLCVGDEIIWVP
                     GFRPAHRYRLKETTHNVIYFSVQHPPEKIAPDENPHS"
     gene            complement(2422..>2814)
                     /gene="mce"
                     /locus_tag="PLT08_12095"
     CDS             complement(2422..>2814)
                     /gene="mce"
                     /locus_tag="PLT08_12095"
                     /EC_number="5.1.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA epimerase"
                     /protein_id="HNE05256.1"
                     /translation="KAINHVAVVVEDMEKSLAFWRDALGIKLHELRDVPAEKSQVAFL
                     PLAGAEVELVMPTTDDSGIAKYLAKRGQGMHHLCLEVDDIEGMMAQLKAKNIRLINEE
                     PRTAADGKKYAFIHPESTGGVLVELYQI"
BASE COUNT          758 a          756 c          632 g          668 t
ORIGIN      
        1 atacgcaaga gtaaatacta cacgcaaggt tgattcgtat gacaaaaaac tccgctcctg
       61 cccacatcag cgccaaaacg ctgttacctg ccgccatcaa cggttgggaa atgttcctgc
      121 tcgatcaggg acgctccccc aataccgtca aagcctttct ctcagatgta cggttgctta
      181 cccagttcct gccgccagat caagccattg gcaccgtcac cactgatgat ctcaaccgct
      241 tcttcgagtg gatggaaaaa ggtcgtggcg tggcatgcag ccccaaaacc ttgtcgcgtc
      301 gtatcacatc ggttaagtct ttcttccgct ggcttcatca atacggcacg ttaagcattg
      361 accctgccga aaaagttctg caacgctctg ccattagccc gttgccacag gttcttaccg
      421 acgaagaata tgaagcggtg cttttggcag cagaccgtca gcgccgcgcc aagaagccag
      481 atgcccgccc atataccttg ttgtctctct tacttcaaac tgccatcaag aaaagtgaat
      541 gccttggcat tcatgtcaat cacattgagt tggctggtaa gaacggtccc ttcctttttg
      601 tgcgctatgc cagccctgcc aaccgctaca aagaacgcaa aattgaactg accgatgatt
      661 gggttgaagc ctacaacgaa tacctcacac aatacagccc tgtagaccaa gctttcccct
      721 ggtcaccgcg ccgtttggaa tatctgcttg aagatattag cgaagaagcc ggtttggaaa
      781 aacatctctc cttcgatatg tgtcgctgga catctgcctt acgcgattat cagaatggca
      841 ttgaaccaga taagatccgc caaaaactag gcgtatcaaa aatccagtgg cgcgagttat
      901 tcatcaaact caagcaatta aacggcgaag tcaaataaaa taaaaaatcc ccatccacta
      961 cgaatgggga ttttcatctg gggcaatctt ctcgggcgga tgttgaaccg aaaaataaat
     1021 cacattatga gtcgtctctt tcaaacggta gcgatgtgcc gggcgaaagc ctggaaccca
     1081 aatgatttca tcacccacac acaagagagg ccaacggtca cgggcacgat gcggcagttt
     1141 ctcgttgaca aaaaagtctg aaagtttttg agaatgaccg cccatcccca gcggagaaaa
     1201 atgatcgccc tgacgacgag cccgcaatag aaattcttca ggaagttcat ccgcatccaa
     1261 ccagacctga aactggtcct cgttttcttc ggcttgttct ctcgccaatg ttggcagacg
     1321 ccaactttca cagatcacct tccacccacc agctagtgaa gcctgcccgc ccagtcttaa
     1381 tggaaccgat ttgtcatcca ccatttgggg ccaacggtca aagggaagct cagcataagg
     1441 cgcgcagata tgaatcagcc cggcttcgcg gaacatacgt agacctgctt ttaagtccaa
     1501 acgcggaacc gtagcgctgg aagcattaat aaaatcagaa gcgcgtttca aggtttggta
     1561 tccaatgtcc acgcctggga gaagagtctg catggcatgg cgaatcaggt tacgttggag
     1621 tccgatcggc atggaagata ggcgcaaaac atcgaaggtg ataatctcat cattcgcaga
     1681 aacaacgaca ttgccccagg catgttccag cgtttccata acgaaggcat aatccccttt
     1741 tagggaatgt gacatgcgca aaaccgtctc gcgaaatttt ggattgtatg tttcaagtga
     1801 gggaatgagc aaatgccgaa tgcggttgcg ttgaaagttc aaagactcat tgctcgagtc
     1861 gtaacttggg cgtaagccat taaccgcaca gaacagaacc gtctcttcgc gccacatatc
     1921 cagtaacggg cgaaccactg gaatatctgg atcaaaggat ttgataaccg accggtacga
     1981 catacccttc aagcccgaaa taccacttcc acgcaaaaaa tgcataagga ccgtttctac
     2041 ttggtcatct gctgtgtgcc caacggcaac ggcttgtgcc ttatattttc tagcaagttc
     2101 aaagaggaag cgatatctta acgtccgcgc ggcttcttca atagagagtt tcttttcttc
     2161 tgcataggaa cgcacatcca tgccccccag aatacatagc aagcccaacc gcacggcagc
     2221 tttttctacc atgcgtgcat ctagtgcaga ctcagggcgg agtttgtggt caaagtaccc
     2281 gatgatgaga tggtaccctg cgcggcgaag cgtttccatt aggcagagac tgtctggtcc
     2341 gccggaaaca ccgacgatga caggacggtc ttttaccaga ccacattcct ctaccatgat
     2401 tgtttcaacg tttgacgaca tttagatttg atacaactcc actaaaacgc cgcccgtcga
     2461 ctcagggtgg atgaaagcat acttcttccc gtctgctgcg gtgcgcggtt cttcattgat
     2521 gagccgaatg tttttcgctt tcaattgtgc catcattccc tcaatatcat ccacttcaag
     2581 gcataaatga tgcatccctt gcccacgttt ggcaagatac ttggcaatgc cagagtcgtc
     2641 ggtggtgggc atcaccaatt ccacttcagc gccagccaaa ggcagaaacg ccacttgaga
     2701 tttctcggcg ggcacgtctc ttaattcatg tagtttaatt cctagggcat cgcgccaaaa
     2761 cgcaagcgac ttttccatat cctcgacaac aacggcaaca tgattgatag cttt
//
LOCUS       DAOQJE010000478         2809 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_24731_length_2809_cov_6.055556, whole genome shotgun sequence.
ACCESSION   DAOQJE010000478 DAOQJE010000000
VERSION     DAOQJE010000478.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2809)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2809)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2809
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24731_length_2809_cov_6.055556"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..188
                     /locus_tag="PLT08_12100"
     CDS             <1..188
                     /locus_tag="PLT08_12100"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNE05257.1"
                     /translation="QQKIFQKFFRSDDPKAREAPGTGLGLNITKSLVEMQGGQIWFES
                     EYRHGTTFHFTVPVAEE"
     gene            195..1355
                     /locus_tag="PLT08_12105"
     CDS             195..1355
                     /locus_tag="PLT08_12105"
                     /inference="COORDINATES: protein motif:HMM:NF020084.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FIST N-terminal domain-containing protein"
                     /protein_id="HNE05258.1"
                     /translation="MAIVSSVGTAQALDGREAGLQATHQALNKLGANVPGLAVVIASH
                     QYQAREVLNGVSSLLGDTPMIGFSSPAGLTRDGLHPHSVVVALLSGDFQAETLWLPGY
                     AQSGRETAQKIEQHASARVERQSVLFFADGFNGDAEQFCNSLPQALDVTGALSSGDLH
                     TGNTYQLMGAQTGAGCMTTAFLRGNLHIGVGSDHGWDPVGNQFRVTRSRGFWLRTLDG
                     RPASEAYAGLFGQPARDWAFPPLSYLARLYPLGMEQSDGMVVRAPIRVEADGSFRMNA
                     VIRDGVDAFLLVGSRASCERAAKTAAQHALLKLGDAKPRLALVLVDVAWQMLLKAQPG
                     AEIAAVQEILGDNVPVAGGYTLGQVVTGDNETKPKFLNQHIIVIVFGEPKEQ"
     gene            1499..>2809
                     /locus_tag="PLT08_12110"
     CDS             1499..>2809
                     /locus_tag="PLT08_12110"
                     /inference="COORDINATES: protein motif:HMM:NF017539.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NACHT domain-containing protein"
                     /protein_id="HNE05259.1"
                     /translation="MTDKKAPTKKPTKKQTPEQDIQAGDSSVAVGGSVTDSAIIHGQT
                     IVLADRFWRDLKPALPPDAIKEATAAFLAYLTDRHYYLSLKGMGVSDRVPLRLKLLDL
                     YVPLKARMELPEGETWKRDLSLAGRDIAEYEEPLHFGDPVPLLNVLKSYSGVVVLGDP
                     GAGKTTFVKYLAVRLARGEGAKMGLDDYLPILLPLAAFANALQSDDIRLDDFIADYFS
                     GIGADLPIGPMLKEALKAGRALILLDGLDEVRDINMRNTVVERVVDFYTFHRHQGNKF
                     VLTSRVVGYRAVRPSAEDLAECTIVDFEDDEIEDFVERWTTALEKQAQGHTQVAQADA
                     EADRRDLLDAIQHNAGVRQLASTPLLLTILALMKRQGVTLPERRVQLYDQYVSTLLST
                     WNRARSLSGRAPGRDIDEIQTVRILAPLALWMHEVSPGVGLVGRE"
BASE COUNT          651 a          716 c          763 g          679 t
ORIGIN      
        1 atcagcaaaa gatcttccag aaattcttcc gttccgatga ccccaaggct cgtgaagcgc
       61 cagggaccgg tctcggtttg aacattacca agagccttgt agaaatgcag ggcgggcaga
      121 tctggttcga aagtgaatat cgacatggca cgacattcca tttcactgtg ccggtagcag
      181 aggagtaatc agcgatggcg atagtctctt ctgttggaac cgctcaggcg ttggatggac
      241 gcgaagccgg gttgcaggca acacatcagg cgttgaacaa gcttggggcg aatgtccccg
      301 ggctggcagt ggtgatcgcc tctcatcaat atcaagcgcg tgaagtgttg aatggcgtat
      361 ccagtttgtt aggtgataca ccgatgatcg ggtttagctc tcctgccgga ttgacgcgtg
      421 atgggcttca tccacactcg gtggttgtgg cattactcag cggtgatttt caggcagaga
      481 cgctttggtt gccggggtat gcccaatctg ggcgtgagac ggctcaaaaa atcgaacaac
      541 acgcttctgc gcgcgtggag cgtcagtctg tgcttttctt tgcagacggt ttcaatggcg
      601 atgcagaaca gttttgcaat tcacttccac aagctttgga tgtgaccggc gcgctttcaa
      661 gcggcgattt acacacgggc aatacctatc aattgatggg cgcgcaaacc ggcgctggtt
      721 gtatgaccac tgctttcttg cgtggcaacc ttcatatcgg tgtcggctca gaccacggtt
      781 gggaccccgt tgggaatcaa ttccgtgtca ctcgttcacg cggtttctgg cttcgcactt
      841 tagatggtcg tccagcttct gaggcgtatg ctggtttgtt tgggcaacct gctcgcgatt
      901 gggcattccc accattgagc tatcttgcgc gtttgtatcc gcttggtatg gagcaaagcg
      961 acggcatggt tgtgcgtgcg ccgattcgag tggaagccga tggtagcttc cgcatgaacg
     1021 cggtgattcg tgatggcgtg gacgctttct tattggtggg gagccgcgcc tcttgcgaac
     1081 gtgctgccaa aacagcagct caacacgccc ttctcaagtt gggggatgca aaaccccgcc
     1141 tcgcgcttgt gctagtagat gttgcctggc aaatgctgct caaagcccag cccggtgccg
     1201 agatcgccgc tgtgcaagaa atccttggcg ataatgtacc cgtggcaggc ggctatactc
     1261 ttgggcaagt ggtgactggc gataacgaaa ccaagcctaa atttctcaac caacatatca
     1321 tcgtcatcgt ttttggcgag cccaaagaac aataaaataa agtacaaaag ccgcccgaaa
     1381 ggcggctttt tcgtatagtc atattagggc tttcccagaa acacccttcc ttgcaagatg
     1441 gtaaagccac ccatacccag attattgtat actgaatcta cacacatcga ggccgggtat
     1501 gactgacaaa aaagccccaa ccaagaaacc aactaaaaaa caaacaccgg aacaagacat
     1561 tcaggcggga gattcaagcg ttgccgtagg cggttccgtc acggatagcg ccattatcca
     1621 tggacagacg attgtgcttg cagaccgctt ctggcgggac ctcaagcctg ctctgcctcc
     1681 cgatgcgatc aaagaagcca ctgcggcttt tctcgcctat ctcacagacc gacattacta
     1741 tttgagcctg aaaggtatgg gggtctcaga ccgtgttcca ctgaggctaa aactgctgga
     1801 cctctatgtt ccgctaaaag cgcgtatgga actcccagaa ggggagactt ggaagcgtga
     1861 cctaagccta gctgggcgcg atatagcgga atatgaagag cctttgcatt ttggtgaccc
     1921 cgtcccattg ctgaatgttc ttaagagtta ttctggggtc gttgtattag gtgaccctgg
     1981 cgctggtaag acgaccttcg tcaagtacct tgctgtccgt ttggcgcgtg gggaaggcgc
     2041 aaagatgggg ctagatgact atctcccgat cttgctccca ttggcagcat ttgcaaatgc
     2101 actacagagt gatgacatcc gcttggatga tttcattgcc gattatttct ctggcattgg
     2161 tgccgacctt cccattggac ccatgctcaa agaagcactc aaagctgggc gtgcgctcat
     2221 tttgttagac ggactcgatg aagtccgtga tatcaacatg cgcaatacag tggttgaacg
     2281 ggtagtagac ttttatacgt tccaccgtca tcagggcaat aaatttgtgc tcaccagccg
     2341 cgtagtaggg tatcgcgcag tacgcccctc ggctgaagat ttagctgaat gcaccatcgt
     2401 tgactttgaa gatgacgaga tcgaagattt cgtagaacga tggacaactg ctcttgagaa
     2461 acaagcacaa gggcataccc aagtggctca agcagacgcc gaagcagacc gccgtgacct
     2521 cttagatgcc attcaacata atgcaggtgt acgtcaactt gcttccaccc cattgttgct
     2581 caccattctg gcgttaatga aacgtcaggg ggtgaccctg ccagaacggc gcgtacagct
     2641 ttacgatcaa tacgtctcca ctcttctctc cacttggaat cgtgcccgca gcttaagcgg
     2701 acgcgctccc ggacgtgaca tcgatgagat tcaaaccgtg cgtattctcg cgccgttagc
     2761 cttgtggatg catgaggtga gcccgggcgt agggctggtg gggcgtgaa
//
LOCUS       DAOQJE010000479         2808 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_24753_length_2808_cov_6.280785, whole genome shotgun sequence.
ACCESSION   DAOQJE010000479 DAOQJE010000000
VERSION     DAOQJE010000479.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2808)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2808)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2808
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24753_length_2808_cov_6.280785"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..706)
                     /locus_tag="PLT08_12115"
     CDS             complement(<1..706)
                     /locus_tag="PLT08_12115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016866 - intramolecular transferase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase family protein"
                     /protein_id="HNE05260.1"
                     /translation="MTLLDQFNKWKETTLKKSLDKFKERKGRFETSAGIEVPRLAIPD
                     GNEGRGDLAPTVADENYVNKLGFPGEFPYTRGVQPTMYRSRFWTMRQYAGFSTAEESN
                     KRYRYLLAQGQTGLSVAFDLPTQIGYDADDPIAQGEVGKVGVSISSIKDMEQLFDQIP
                     LDKVSTSMTINAPAGVLLAMYIAVAKRQGADMRELRGTIQNDILKEYVARGTYIFPPA
                     PSMRLITDIFQFCSKEV"
     gene            1247..2119
                     /locus_tag="PLT08_12120"
     CDS             1247..2119
                     /locus_tag="PLT08_12120"
                     /inference="COORDINATES: protein motif:HMM:NF024860.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase"
                     /protein_id="HNE05261.1"
                     /translation="MKRIIVAGIYRSGTSLITELVHRWGAYAGREEDIFKDEFGYMEH
                     FALQKLNDELLNNNSRVPTPVETLLEKAEDAGLRERALSILAEMDEEAQANHAAAWVW
                     KDPRLPLVLPFWADVWGDVIYVIPVRHPVETIFSAAKMDGLDPEQLPLSAGLAYWQFC
                     MLNLLQYTANTPRKLFMAYDQLTQDPQRECERLCRFLDAQCEVDSDPSDRIRSMTEQV
                     AAGERHFNHPRSLAEVETSTREQRALYDFLRVKTLYPDEPFHKDDFAMYPGWFEYLSA
                     MDMLFSAGDAGQEA"
     gene            2123..>2808
                     /locus_tag="PLT08_12125"
     CDS             2123..>2808
                     /locus_tag="PLT08_12125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE05262.1"
                     /translation="MSPMSLEAFLALPTEELANLVRGDRVKTCVFPFNGTRRWFLLER
                     PSSAGGDLAKQYIELTTQGYINTYKLVFDHGIDNIVAPAFGGELLVNRGEEYMRQIGT
                     SLSLLAIHPDFVNFYKEYDVRVHFYGEYRKQFKDTSYAYIIDQFDEVTRQTADHTSRA
                     LFYGVFAEDATMTTAELAIQHVQQTGTHPTRRDLIERYYGEFIEKADIFIGYEKFNVF
                     DYPMLGLGEE"
BASE COUNT          699 a          707 c          665 g          737 t
ORIGIN      
        1 gcacttcctt cgagcagaac tggaaaatgt ccgtgatcag tcgcatcgac ggggcgggcg
       61 ggaagatata cgtcccacgg gctacatatt ctttgaggat gtcgttctgg atcgtgccgc
      121 gtagttcgcg catgtccgcg ccctggcgct tggcaaccgc aatatacatc gccagcaaga
      181 ctcccgccgg cgcgttgatg gtcatggatg tcgaaacttt atctaatgga atttgatcga
      241 ataactgctc catgtctttg atcgacgaaa tggacacgcc caccttaccg acctctccct
      301 gcgcgatggg atcatccgca tcatacccaa tttgagtggg gaggtcgaac gcaaccgaca
      361 gccccgtttg accttgtgcc agcaaatagc ggtagcgctt gttcgattct tctgccgtgg
      421 aaaagcctgc atactggcgc atcgtccaaa aacgtgaacg gtacatcgtc ggctgtacgc
      481 cgcgggtgta gggaaattct ccggggaaac caagtttgtt tacataattt tcatccgcca
      541 ccgtaggggc gaggtcccct cgcccttcat ttccatcggg aatcgcgagg cgtggcactt
      601 cgatcccagc gctggtttca aaacgtccct tacgttcttt gaatttatcc aaagacttct
      661 tgagcgtagt ttctttccat ttgttgaact ggtctaataa agtcatgttt cacctttgtt
      721 ttcgtggtga ctgcccctca agtataaagc gaaccccgaa taacttgtag gataagtagc
      781 accccctgcc gcgaaaaacc tccttttttc tacattacca aaatttaaca tgtttcttgc
      841 atttttgtta aattctaaag tcgtatatac ctgtaaaata attaatgtaa caagcattga
      901 atcaaggttc tatacaacgt tgcttaaccg cccttcaacg acactcctgc aatatacgtg
      961 ggatgcaaga ctgtaagccc ttctatttaa taacatatcc agaggggaaa ttgaccgggc
     1021 gcacaaaatc caaaaagagc cgcttcttac tccgcccatg cttgggcaag cctttctgag
     1081 agccatccct tcgccgaaac attttgcatt tgagcctatg ggttgtgcct aaagatgggg
     1141 gattgttgta tatgaattcc tgaagagatc agcgttctta attttgttca taccgtagaa
     1201 gagttattaa ctttatactt tttgatacat aaaaggacaa atgacaatga agcgtatcat
     1261 cgttgccgga atttatcgaa gtgggacatc actaatcaca gagttggtcc atcgttgggg
     1321 ggcgtatgca gggcgggaag aagatatttt caaagacgaa tttggctata tggagcactt
     1381 cgccttacag aaattgaacg atgaacttct aaacaataac agccgcgttc ccacacctgt
     1441 agagaccttg cttgaaaaag cagaagatgc cgggttgcga gaacgtgcct tgagcatctt
     1501 agctgaaatg gatgaagagg cgcaggcgaa tcatgccgcg gcatgggttt ggaaagaccc
     1561 acgcttgcct ttggtgcttc ctttttgggc agatgtatgg ggtgatgtta tctatgtaat
     1621 tcccgtgcgt cacccagtag agacgatttt ctccgctgcc aaaatggatg ggctcgaccc
     1681 ggagcaactt ccgctttcag ctggcttggc atactggcag ttttgcatgt tgaacttgct
     1741 ccaatacaca gccaataccc ctcgcaaact attcatggcg tacgaccaac ttacgcaaga
     1801 cccacagcgt gaatgtgaac gcctgtgccg ttttctcgat gcgcaatgtg aagtcgatag
     1861 cgacccatcg gaccgtattc gttccatgac cgaacaggtc gcagcggggg agcgccactt
     1921 taaccatcca cgctctctgg cagaagtgga aacatccacc cgtgagcaac gggcgttata
     1981 tgatttcttg cgcgtcaaaa ccctttaccc cgatgagcct ttccacaaag acgatttcgc
     2041 catgtacccc ggttggtttg agtatctctc tgccatggat atgctcttct ctgccgggga
     2101 tgctgggcag gaggcataag aaatgtctcc tatgtctctt gaagcttttc ttgccctgcc
     2161 taccgaagag ttggcaaacc ttgtccgcgg cgatagggta aaaacctgtg tctttccttt
     2221 taatggcacg cgccgctggt ttttgttaga gcgtccatca tctgctggcg gcgatcttgc
     2281 caaacaatac atcgagttga ccacgcaagg atatatcaat acctataaat tggtctttga
     2341 ccacggcata gataacatcg tcgccccggc ttttggcggc gagttattgg tcaaccgcgg
     2401 cgaagaatac atgcgccaga tcggaaccag cctgtctttg ctggcaattc acccagactt
     2461 tgtcaacttc tataaagaat atgatgtacg cgtccacttt tatggagagt atcgcaagca
     2521 gtttaaggat acttcctatg cctacatcat tgatcaattc gatgaggtca cacggcagac
     2581 ggcagatcat acgtctcgcg ccttgttcta tggggtcttt gccgaagatg ccaccatgac
     2641 aaccgcagag cttgccattc aacacgtcca acaaacgggt acgcacccca cccggcgcga
     2701 cctgatcgag cgttattacg gcgaattcat tgaaaaagcg gatattttca tcggctacga
     2761 aaaattcaac gtcttcgact atcccatgct tggcttggga gaagagag
//
LOCUS       DAOQJE010000480         2805 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_24792_length_2805_cov_6.525455, whole genome shotgun sequence.
ACCESSION   DAOQJE010000480 DAOQJE010000000
VERSION     DAOQJE010000480.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2805)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2805)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2805
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24792_length_2805_cov_6.525455"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(65..1009)
                     /locus_tag="PLT08_12130"
     CDS             complement(65..1009)
                     /locus_tag="PLT08_12130"
                     /inference="COORDINATES: protein motif:HMM:TIGR00147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0001727 - lipid kinase activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YegS/Rv2252/BmrU family lipid kinase"
                     /protein_id="HNE05263.1"
                     /translation="MRKGLLLYNPAAGRFPVRRHVRGIIKPLNDAGWNMDIAETLSGT
                     HAVQTAHQAAQEKYDAVFAIGGDGTVGQVASGLIHTETALGVLPAGTVNVWALEQGQK
                     PYSWWQPGNLKENAKLLSNVEPQYVDVGDCNDQPFLLWAGVGLDAHTINKIEPRPRIF
                     KHIAFPHYFVTTVWEATFWHGLDLRIYAEGKLVEGHYLVAVATNIRHYAGGQSLISPN
                     ALLDDNEMDLWLLSGSNVADALRHYFDLVAGRHLTSEEARCIPFHHVTIESDANFSIQ
                     VDGDPMLGGHKAEIKVKHRALKVLVPENALYLLKTPQR"
     gene            1120..1455
                     /locus_tag="PLT08_12135"
     CDS             1120..1455
                     /locus_tag="PLT08_12135"
                     /inference="COORDINATES: protein motif:HMM:TIGR00377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HNE05264.1"
                     /translation="MEITTQEFKHCDLIAVKGRVDSATAPQFAKALETANDNGRFKLA
                     LDMSELEYMSSAGFRALLAAQRNCKRYNRGEVVLVRVPDRIREALELAGFTELFKTFD
                     DTVTAVGSF"
     gene            1525..1932
                     /locus_tag="PLT08_12140"
     CDS             1525..1932
                     /locus_tag="PLT08_12140"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF024970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNE05265.1"
                     /translation="MPAYTFPAKFEFLDEIRDTVAEIARSGGFSEKTIYSLQLAADEA
                     ASNIIEHAYEGVSNATLYMTCDMKGDEIVITMRDTGKSFNPTTVKEPNLKADLSERQI
                     GGLGVYLMRKLMNTVHYESSKSGNLLTMTKRRE"
     gene            1937..>2805
                     /locus_tag="PLT08_12145"
     CDS             1937..>2805
                     /locus_tag="PLT08_12145"
                     /inference="COORDINATES: protein motif:HMM:NF024583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HNE05266.1"
                     /translation="MSAATRSSKDWLQLASLGEQLRSEDSLSAQRDRIAAMANRLIEG
                     KVDVWLHENIFRLPDWDDGYIFPTQPALDGMKRAIKTGKLARKKKSSGKKPPASRSTF
                     AAIPLEDQGITLGALQVSRSKGPEFTDDELNLLQGLGHIISVNLFASHRAEVEQFRLR
                     QLNLVREVSAQIANVLDVDDLASQVTRLIQKTFNFYYVAFFTLDPQDNALHFRASAVA
                     PRKGRRKASIALEVEIGQGLIGEAAKTGMEIVCDDVQSDSRYRFIDSLPETRSEVVIP
                     LKIEDHILGALDV"
BASE COUNT          729 a          698 c          695 g          683 t
ORIGIN      
        1 atcccggcgg tgatcaatga tcccacaatg acgaagcgat ttagataggg ttttaagtcc
       61 attattatct ctgcggtgtt ttcagcaagt ataaagcgtt ttcgggtact aacactttga
      121 gcgcccggtg ctttactttg atctctgctt tgtgcccgcc aagcatgggg tcgccatcta
      181 cttgaatgga gaagttcgca tcagactcaa tggtgacatg atggaacggg atgcagcgtg
      241 cttcttcaga ggtgagatgt ctgccggcta caaggtcaaa gtagtgacgc aaggcatcgg
      301 caacattgct gccgcttaat agccataaat ccatttcatt atcatcgagc agagcattgg
      361 gagagataag agattgtccg cccgcatagt gccgaatgtt ggtggctacc gcaactagat
      421 aatgcccttc cactaacttt ccttcggcat aaattcgcag gtctaaccca tgccagaagg
      481 tggcttccca gacggtggtt acgaaataat ggggaaatgc aatatgcttg aaaatgcgcg
      541 gacgtggctc gatcttgttg atggtgtgtg cgtctaagcc aacgcctgcc cacaatagaa
      601 aaggctgatc gttgcaatcg cctacatcta catattgcgg ttcaacattt gacagtaact
      661 ttgcgttttc ttttaagttg ccaggctgcc accaactata gggcttttgc ccttgctcta
      721 atgcccaaac atttacggtt ccagcaggga gtacgcccag cgcggtttcg gtgtgaatca
      781 acccgctggc aacttgccct acggtgccat cgccaccaat ggcaaagacc gcatcatatt
      841 tttcttgtgc cgcttggtgc gcggtttgta cagcatgggt gccgctgagt gtttcagcaa
      901 tatccatgtt ccagccggca tcattaaggg gtttgatgat gccacgcaca tggcggcgaa
      961 ccggaaaacg ccccgcggcg gggttgtaga gtaagagtcc cttgcgcatg ggtggggatt
     1021 atagccgatg ggggatggga ggatatataa aaaattcacc tatctacgtg atataatccc
     1081 gctaattcat cactcgatat cttgttcgga ggagaaatta tggaaatcac gacccaagaa
     1141 ttcaagcact gtgacctgat tgcagtaaaa ggacgggttg acagcgcgac agctccccaa
     1201 tttgccaagg cacttgaaac tgccaacgat aacggacgtt tcaaacttgc ccttgatatg
     1261 agcgaattgg aatacatgtc cagcgcaggt ttccgtgcct tgctcgcagc acaacgcaac
     1321 tgcaaacgat acaatcgcgg cgaggtggtg cttgttcgtg ttccagaccg aattcgcgaa
     1381 gccctcgaat tggcaggctt caccgaactc ttcaaaacct tcgacgacac cgtaaccgcc
     1441 gtcggaagtt tctaatagaa gggttcctaa aaagaaccga catgccgtct ctttaaagac
     1501 aagggacggc atctcatttt ctttatgcca gcatacacat tcccagccaa gtttgaattt
     1561 ctggacgaga tccgcgatac agttgcagag attgcgcgca gcggagggtt ctctgaaaag
     1621 accatttact ccctccaact tgccgcggac gaagctgctt ctaacatcat tgaacatgcc
     1681 tatgaaggtg tgtcgaacgc tactttgtat atgacgtgtg atatgaaagg ggacgagatc
     1741 gtcatcacca tgcgagatac tggaaaatca ttcaacccaa ccacggtcaa agaaccgaac
     1801 ctcaaagccg atttatcaga acggcagatc ggcgggctgg gcgtatatct catgcggaag
     1861 ttgatgaaca ccgtccatta cgaatcttcc aaatctggca atctactgac aatgaccaaa
     1921 cggagagaat agtttcatga gcgccgcaac ccgatcctct aaggattggc ttcaactcgc
     1981 cagcttgggt gagcaactgc ggagcgaaga ctctcttagc gcgcaacgag atcgcatcgc
     2041 tgctatggca aaccgcctca tcgaaggcaa ggtggatgtc tggctgcacg aaaacatctt
     2101 ccgcctgcca gattgggacg atggatatat cttcccaacc cagcctgcat tagacggaat
     2161 gaaacgtgcc ataaaaacgg ggaaactggc tcgtaagaaa aaaagcagcg ggaagaaacc
     2221 ccccgcctct cgctcgacct ttgctgcaat tcccctcgaa gatcagggga ttacgctggg
     2281 agcgcttcaa gtatcccgct caaaaggacc agagtttaca gatgatgaat taaacctgct
     2341 tcaaggcttg gggcatatca tctctgtaaa tttatttgcc tcgcaccgcg ccgaagtgga
     2401 acaattccgc ttgcgacaat tgaacttggt acgcgaggtc agcgctcaga tcgccaacgt
     2461 gctcgatgtg gacgatctag catctcaagt aacaagactt atccaaaaga cctttaactt
     2521 ttattacgtt gcctttttca ctctcgaccc tcaagataat gcgcttcact ttcgtgctag
     2581 tgccgttgca ccgcgtaaag gtcgccgtaa agcatccatt gcattggaag tggagatcgg
     2641 tcaggggttg atcggtgaag cagcaaagac cggcatggag atcgtctgtg acgatgttca
     2701 gtcggattct cgttatcgct ttattgatag cctcccagaa actcgctctg aagttgtcat
     2761 tccgctcaag atagaagacc atattctggg cgcattggat gttca
//
LOCUS       DAOQJE010000481         2803 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_24824_length_2803_cov_5.025473, whole genome shotgun sequence.
ACCESSION   DAOQJE010000481 DAOQJE010000000
VERSION     DAOQJE010000481.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2803)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2803)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2803
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24824_length_2803_cov_5.025473"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..667)
                     /locus_tag="PLT08_12150"
     CDS             complement(<1..667)
                     /locus_tag="PLT08_12150"
                     /inference="COORDINATES: protein motif:HMM:NF013112.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE05267.1"
                     /translation="MKVFLDTIGCRLNQAEIESMSRQFRAAGHEIVASAENADMAVVN
                     TCAVTTQAASDSRGKIRTIARAGVNEIVATGCWTSIQPKEAAALPNVKHVISNDKKDL
                     LVPEILGLPKEKFDVEPIDRIPIPGLHRRTRAFIKVQDGCDNKCTFCVTTIARGEGRS
                     RPLADVINDVNSALAGGSKEIVLTGVHLGSWGQDFGNHLRELVQALLRETDVKRLRLS
                     SLEP"
     gene            complement(686..1339)
                     /locus_tag="PLT08_12155"
     CDS             complement(686..1339)
                     /locus_tag="PLT08_12155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE05268.1"
                     /translation="MNRSVSLVFVLMFLLSACGGAPANQTQAPAAALPSGSVLFQDDF
                     SQPTTGWDRLLVTEGVMDYYSGGYRMLVNSLQTNFWATPRRNFTDVRMEVDTGKLAGP
                     DENRVGLLCRYNGKDYYFFIMTSDGFYGIGLYKDGQATLLGQSEMLASSNINKGVAVN
                     HLRADCTGNILTFYINGFQVTQAQDTTLPSGDIGLIAGTFAEPGVDILFDNFVVLQP"
     gene            complement(1353..2747)
                     /locus_tag="PLT08_12160"
     CDS             complement(1353..2747)
                     /locus_tag="PLT08_12160"
                     /inference="COORDINATES: protein motif:HMM:NF024820.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNE05269.1"
                     /translation="MLLQRAKPYFIGLLLVIAIIIPFLVAGYTSLNRAENAYSTEQYR
                     LAAEEYAHTARILFWRKDLYGRAGISAAQGEDFEGAIAYFKQAKNLTEMGWVLFCQSY
                     AQLQDYPNTISTCKAGLQKYQSAPLYKILAFVYRDQKDWVAELDALKHQTQLDPNDAY
                     AAYRLGVLMALYAPEDSIPELTRASSLNPEVDSAVQTLRAALAVSSQQSDPSMQKVIV
                     GQAFGLVQDWELAKTAFEQAVQQNENNAEAWAWLGEAKQQTGQDGSAELDQALLLDHN
                     SVNVRALRGLYWSRKGKYSQMLAEYSLAARIEPENPRWQASLGEAYSKIGDLVAAMAA
                     YQRAVELDPTQADYWRLLAIFCAENNVHAEDVGIPAAQQAYTLSPNDPASLDALGYAY
                     LSTGRYASAELAFSQAIEISAQYYPAHIHLAMNYLAQGNRAAAFNVLTYVRDADGAGV
                     YAETAKQLLDKYFQ"
BASE COUNT          757 a          698 c          648 g          700 t
ORIGIN      
        1 aaggttcaag ggaagaaagg cgcaggcgtt taacatccgt ttcacgcaaa agagcttgga
       61 ctagttcacg aagatggttg ccaaaatctt gaccccatga gccaagatgc acgcccgtta
      121 gaacaatttc ttttgaacca cctgccaatg ccgagttgac atcattgatc acatctgcca
      181 atggacgcga ccgcccttca ccacgcgcaa tggtagtgac acaaaatgtg catttgttat
      241 cgcagccatc ttgtactttg atgaaagcac gggtgcggcg atgcaatccc ggaataggaa
      301 tgcggtctat tggctcaaca tcaaatttct ctttgggcaa gccaaggatc tcaggcacta
      361 gcaaatcttt tttatcattg gaaataacat gcttgacgtt tggaagtgcc gccgcttctt
      421 ttggttgtat cgaagtccaa cagcctgttg cgacgatttc attgacccca gcacgagcaa
      481 tagtacgaat ctttccccga gagtcagatg cggcttgagt tgtgactgcg caagtgttaa
      541 ccactgccat atctgcattc tcagcagaag ccacaatttc atgccccgcc gcacggaact
      601 ggcgagacat ggattcaatt tctgcctgat tcaaacggca gccaatggta tcgagaaaga
      661 ctttcataaa caggtgttac gtatattaag gctgcaatac aacgaagtta tcaaaaagaa
      721 tatcaacgcc gggttcggcg aaggttccgg caatcaagcc aatatcaccc gaaggcaaag
      781 ttgtatcttg agcttgcgtc acctgaaaac catttatata gaaggttaag atattccccg
      841 tgcaatcggc tcgcagatga ttgaccgcaa cacctttgtt gatattcgag gaagcaagca
      901 tttcagactg tcccaacaaa gttgcctgac catctttata caaaccgatc ccgtaaaaac
      961 catcgctggt catgataaag aaatagtaat ctttgccgtt ataacggcaa agcagaccca
     1021 cacggttttc gtctggacct gctaatttac cggtgtccac ttccatgcgg acatcggtga
     1081 aattacggcg tggcgttgcc caaaaatttg tctgtaacga attaacgagc atacgatagc
     1141 cgccagagta ataatccatt accccttcgg tcacgagcaa acgatcccag ccagttgttg
     1201 gctgagaaaa atcatcttga aaaagaacag accccgaagg caaggctgcc gctggagcct
     1261 gagtttgatt tgcaggggcg ccgccgcatg cagaaagtaa aaacattaga acgaatacta
     1321 aagatacaga tcgattcatg agcgtgattt tatcattgaa aatatttgtc taataactgc
     1381 ttggcggttt cggcatatac gcctgcgccg tcggcatcgc gcacatacgt cagtacatta
     1441 aaagccgcgg cacgatttcc ttgtgcgaga tagttcatcg caaggtgaat atgagctgga
     1501 taatactgag ccgaaatttc aatcgcttgt gaaaaagcca attctgcgct ggcataacgc
     1561 cctgttgaaa gataagcata gcccaaagca tccaaagaag ccgggtcgtt gggtgaaagg
     1621 gtataggctt gttgtgcggc aggaatacca acatcttctg catgtacgtt attctctgcg
     1681 caaaaaatcg ccagcaagcg ccaatagtcg gcttgcgtgg gatcaagttc taccgcacgt
     1741 tgatatgccg ccatagccgc caccaagtcc cctatttttg aataggcttc acccaagctt
     1801 gcctgccaac gcggattctc cggctcaatg cgcgccgcaa gcgaatactc tgccaacatt
     1861 tgcgaatact tgcccttgcg actccagtac agtccacgca aagctcgcac attgaccgaa
     1921 ttatggtcta ggagtaatgc ctgatcaagt tccgcgcttc catcttgacc tgtttgctgt
     1981 ttcgcctctc cgagccatgc ccacgcctca gcgttgtttt cattttgttg aaccgcttgc
     2041 tcaaatgctg tttttgccaa ttcccaatct tgtaccaagc cgaatgcctg ccccacgatc
     2101 accttctgca tagaaggatc gctttgctgg ctggaaactg ctaaagcggc gcgcaaggtt
     2161 tggacagcag aatctacttc ggggttaaga gaggaggcgc gtgtcagctc cgggatggaa
     2221 tcttccgggg cgtacaaagc catcagcacc ccaagtcgat acgcggcata ggcgtcgttt
     2281 gggtctaatt gagtttggtg cttaagtgca tcaagttctg ccacccaatc tttttgatct
     2341 cgatacacaa acgccaaaat tttatataac ggtgcagatt gatatttctg aagccccgcc
     2401 ttgcaagttg aaattgtgtt tgggtaatct tgcagttgcg catacgattg gcaaaacaag
     2461 acccacccca tttcagtgag gttcttggct tgtttaaaat atgcaatggc gccttcaaaa
     2521 tcttcgcctt gcgccgccga aatacccgca cgcccataca gatcctttcg ccagaacagt
     2581 atgcgggcag tatgggcata ttcttcagcc gcaagccgat attgctcggt agaatacgca
     2641 ttttcagccc ggtttaggct ggtatagcct gccacaagga acggaatgat gatggcgatg
     2701 acaagcaaaa gacctataaa gtagggtttg gctctttgta gcagcataat ttcaattcta
     2761 cgccataatc cctaactggc agtataatca cgagcggaat gag
//
LOCUS       DAOQJE010000482         2800 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_24861_length_2800_cov_3.851002, whole genome shotgun sequence.
ACCESSION   DAOQJE010000482 DAOQJE010000000
VERSION     DAOQJE010000482.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2800)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2800)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2800
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24861_length_2800_cov_3.851002"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(339..>1533)
                     /locus_tag="PLT08_12165"
     CDS             complement(339..>1533)
                     /locus_tag="PLT08_12165"
                     /inference="COORDINATES: protein motif:HMM:NF012766.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="M24 family metallopeptidase"
                     /protein_id="HNE05270.1"
                     /translation="SMSLIQEKTDQAVQILKEQDIDLWLTFVRETSGVRDPVLSFIIG
                     EADLTWPSALIMTKTGDKIAIVGNLETEALQRLDVFTQIIGYDTGVSGVLRETISKLN
                     PNQIAVNMSRNNVHADGLTHAMYEFLVEYLSGTPYPERIISAEPVINALRGRKTKAEL
                     ARIQRAVEITDEIYKKTFAFIKVGMTEIQIADYMQALMKEYGVGYAWPRENNPAVNSG
                     PDKVVGHNGPTDIKVEPGHIIHFDFGVKHEGYCSDIQRIAYVLREGETEAPVEVQRGF
                     ITVRTAIEKSREAMKAGVIGNDIDTISREIIVDSGYPEYMYALGHQLGRVAHDGGALL
                     GPRWEKYGDSPNQKLEVGQVFTIEPGLAVPHYGYVGLEEDVVMTAEGALYVGDPQREI
                     VLIGR"
     assembly_gap    1534..1633
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1634..2691)
                     /locus_tag="PLT08_12170"
     CDS             complement(<1634..2691)
                     /locus_tag="PLT08_12170"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNE05271.1"
                     /translation="MLSPLEQAIRKDLKHNVTAGLWDGSLFGVALGFASFGTILPLFV
                     ATMTNSATLIGLVPAIHSAGWLFPQLFTASHVSRLRQLKRTVLLNTIHERIPFLGFAA
                     VALLLPVVGVNAGLLLTFLLLIWQGLGGGFTANAWTSMVSKIIPPESRGTFFGMQAGL
                     ANLFISGSAIAAGYLLNALASPWDFAVCFFIASIFFALSWFALAATREPEDTEKFIPE
                     EKSHFWDDAKKVLSRDTNFNWFLSARILSQFATMGFSFYIIYALREFNMTEVVAGYLT
                     ATLTISQTIANIGMGWIGDRVGHRVMLIIGAFSAFLSALLAWNAASIAWFYPIFILTG
                     LANVSMWTISMTMTVDFG"
BASE COUNT          676 a          651 c          687 g          686 t
ORIGIN      
        1 gcaaggcttc aatacgttcg cgcgccgtca tctttccttt ggcacgctgt ttggcattgc
       61 gttcttcgcc gccaccttcc tttgccttcg cacgaactcg ccgtaattcc aaaattctag
      121 ggtcttcttg agtcatgtta agtcctttag gtgaaaattt tcacaatcga aagtataaca
      181 ttggatgtca gacaagccag tgatggaggt catgtcggaa ataggacaat ccgcctcctc
      241 atttgatttg gggctggttc tgttttgtga gcttttttct gccctgcaag ttgttgctac
      301 aaaaagagct tggacgatat cgcccaagct ctttttgtct atcgtccgat caaaacgatt
      361 tctctctgcg ggtcaccaac gtagagagcg ccttctgccg tcatcaccac atcttcttcc
      421 aaaccaacgt atccgtagtg gggtacagca agacccggct caatagtgaa gacttgaccg
      481 acttctaact tttggttggg ggagtctcca tatttctccc aacgcggacc caacaatgcg
      541 ccgccatcat gtgctacacg cccaagttga tgacccagcg catacatata ttcggggtat
      601 cccgagtcca cgatgatttc gcgtgagata gtatcgatgt cattcccaat cacaccagcc
      661 ttcatcgcct cacgcgattt ctctatcgcc gtgcggacgg tgataaaccc gcgctgaact
      721 tctactggcg cttcagtttc gccttcgcgc aacacataag caatgcgttg aatgtcggaa
      781 caatagcctt cgtgcttcac gccaaagtca aagtggatga tatgacctgg ctcgaccttg
      841 atgtcagtcg gtccattatg cccaaccact ttatcgggtc ctgaattgac cgccgggtta
      901 ttctcgcggg gccaggcata gccaacgcca tattctttca tcaaggcttg catgtaatct
      961 gcgatctgaa tttcggtcat gccaactttg ataaaagcaa acgtcttttt gtaaatttcg
     1021 tctgtgatct caacggctct ttgaatgcgg gcaagttcgg ctttggtctt gcgtccacgc
     1081 agggcgttga tgaccggctc cgcgctgata atgcgttcgg ggtatggtgt gcctgagaga
     1141 tactccacca gaaattcata catggcatgt gtcaacccat cggcatggac gttgtttctg
     1201 gacatattga ccgcgatttg attcgggttg agttttgaaa tggtctcacg cagcacaccg
     1261 ctgacgcccg tatcgtaccc aatgatctga gtaaacacgt ccaagcgctg caatgcttcg
     1321 gtttcaagat tgccgacaat cgcaattttg tctccggttt ttgtcataat cagcgcggag
     1381 ggccaagtca ggtcagcttc accgatgatg aaactcaaca ccgggtctcg gactccgctt
     1441 gtctcgcgga cgaaggtgag ccagaggtca atatcttgct ctttgagaat ttgaacggct
     1501 tggtcggtct tttcttgtat gagggacata gatnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1561 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1621 nnnnnnnnnn nnnttgccga agtctacggt catggtcatg ctgatggtcc acatggaaac
     1681 atttgccaag ccagtgagaa tgaagatggg gtaaaaccat gcaattgaag cggcattcca
     1741 cgcaagtaaa gcgcttagga atgcagagaa tgcgccaatg ataagcatga cgcgatgccc
     1801 aacccggtca ccgatccagc ccatgccaat gttggcaatc gtttgtgaaa tggtaagggt
     1861 tgcggtgaga taccctgcca ctacttcggt catattgaat tcgcgtaggg cgtagataat
     1921 gtagaacgag aatcccatgg tcgcaaattg cgagaggatg cgtgcactca agaaccagtt
     1981 gaagttggta tcgcggctca ataccttttt ggcatcgtcc cagaaatgag atttttcttc
     2041 ggggatgaat ttttctgtat cttcaggttc gcgggtagcg gctagggcga accaggataa
     2101 ggcgaagaaa atgctggcaa tgaagaaaca gaccgcaaag tcccagggag aggcgagggc
     2161 gttaagtagg tatcccgctg cgatggcaga tccgctaatg aagagattcg ccagtccggc
     2221 ttgcatccca aagaaagtgc cacgtgactc cggtggaatg atcttggaga ccattgaggt
     2281 ccacgcattg gcggtaaaac cgccgccaag cccttgccat atcagtagca aaaacgtaag
     2341 cagtaaaccg gcattcacgc caacgacggg gagtaagagt gctaccgccg caaaccctaa
     2401 aaagggaata cgttcatgaa tggtatttag taacacagtg cgttttagtt ggcgtaagcg
     2461 ggagacgtga ctggcagtaa agagttgtgg aaaaagccag cccgccgaat ggatggcagg
     2521 caccaagcca attaaggttg cagaattggt catggtggcg acgaagagcg gcaggatggt
     2581 tccgaaggag gcaaagccta atgccacacc gaatagactt ccatcccata accccgcagt
     2641 gacattgtgt ttaaggtctt tacgaatagc ttgttcaagt ggggagagca taaagtgatt
     2701 ttaccttatg gagttactgg catattgtgg ggcggaggtg tgtgaactta tttttcctcc
     2761 ttgacaaaat aaacctactt ggtctaaaat aaaaacgaac
//
LOCUS       DAOQJE010000483         2799 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_24878_length_2799_cov_5.483236, whole genome shotgun sequence.
ACCESSION   DAOQJE010000483 DAOQJE010000000
VERSION     DAOQJE010000483.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2799)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2799)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2799
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24878_length_2799_cov_5.483236"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..626
                     /locus_tag="PLT08_12175"
     CDS             <1..626
                     /locus_tag="PLT08_12175"
                     /inference="COORDINATES: protein motif:HMM:NF024871.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016755 - aminoacyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0044038 - cell wall macromolecule
                     biosynthetic process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="peptidoglycan bridge formation glycyltransferase
                     FemA/FemB family protein"
                     /protein_id="HNE05272.1"
                     /translation="ILMRMKQKTRYNVRLAEKKGVTVRVGSVDDLPMLYKMYAETSVR
                     DGFVIRDEEYYMMVWKLFLTNYESRITPSTIPLIAEVDNAPVAAIFLFTFAARGYYVY
                     GMSRDKHREKMPTYLLQWAAMKHTKAHNCLTYDLWGAPDVFDESDSMWGVYRFKEGLG
                     GEVVRTLGAYDFAPSKFWYSMYADVMPRVLNFMRSRGKEKTKQGLSA"
     gene            707..1678
                     /locus_tag="PLT08_12180"
     CDS             707..1678
                     /locus_tag="PLT08_12180"
                     /EC_number="4.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016846 - carbon-sulfur lyase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-cysteine desulfhydrase family protein"
                     /protein_id="HNE05273.1"
                     /translation="MNKIARLNFAHLPTSIEELPNLSKALNGPHIFVKRDDQTGLAFG
                     GNKTRKLEFLIAEAREQGADLLISGGAMQSNHCRQTAAAAARYGFECKLVLTGAKPQQ
                     ASANYLLDQLFGAEIITVEDRAFRDQTLQKTFDDALAAGKKPYLVPYGGSSPTGAMGY
                     TFAMEEFMQQNAHADWIVFGTSSGGTHAGLVLGQRLFGYKGKVLGISIDEPEEELKSH
                     VSDLASRASEKLGERIQFSSNDVLATEDYCQSGYGVFGEGEREAIHLFAKHEGLLLDP
                     VYTGRAAAGMIDLIRKGFFKKDEMILFWHTGGQPALFADKYAQDVIR"
     gene            complement(1728..1964)
                     /locus_tag="PLT08_12185"
     CDS             complement(1728..1964)
                     /locus_tag="PLT08_12185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE05274.1"
                     /translation="MDLIDILGWIGSATYLLAFALNSAKKVTSDSFTYQGMNILAGVI
                     MTYYTFSHGAYSATALNSIWAIIGVVTLVNKGTR"
     gene            complement(1964..>2799)
                     /locus_tag="PLT08_12190"
     CDS             complement(1964..>2799)
                     /locus_tag="PLT08_12190"
                     /inference="COORDINATES: protein motif:HMM:NF015438.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MOSC domain-containing protein"
                     /protein_id="HNE05275.1"
                     /translation="TFHSPPRSTRTPMISLSTLTYYPIKACRGFHVPASQVQRMGLEH
                     DRRMMVVTPDGKFLTQREHPRLALVTPTLRNEAVTLSAPNFESIQFAVQSSGTPTPVD
                     IWSSSGVDAIDQGDESATWFSDWLGISVRLVHIADGFKRKLNPLYAVHADDHTGFADG
                     YPILVISEESLQDLNSRLDSAVPMNRFRPNLVVKGCEPFAEDTWKRIRIGSVEMALVK
                     VCPRCVVTTIDKETLAKNKEPLKTLSGYRNHELGAIFGMNTIPLNEGKIEIGMTVEIL
                     E"
BASE COUNT          686 a          699 c          743 g          671 t
ORIGIN      
        1 atatcctcat gcgcatgaaa cagaagacgc gttacaacgt gcgcctcgct gagaaaaaag
       61 gcgtgaccgt gcgcgtgggc agcgtggacg atttgccgat gttgtacaag atgtatgctg
      121 aaacatcagt gcgtgatggc tttgtgattc gtgatgaaga atattacatg atggtttgga
      181 aattgttcct tacgaattac gaatcacgaa ttacgccttc aaccattcca ttaattgcgg
      241 aagtagacaa cgcgcccgtc gctgctattt tcctcttcac cttcgccgcg cgcggatatt
      301 atgtctacgg catgtcgcgg gataaacacc gtgaaaagat gccaacctat cttttgcaat
      361 gggcggcgat gaaacatacc aaggcgcaca actgcctgac ctatgacctg tggggcgcgc
      421 cggatgtctt cgatgaaagc gactcgatgt ggggcgtgta tcgcttcaag gaaggtttgg
      481 gtggtgaagt ggtgcgtacg ctcggtgcgt atgactttgc gccgagcaag ttctggtatt
      541 cgatgtacgc cgatgtcatg ccgcgcgtgt tgaattttat gaggtcgcgt ggcaaagaaa
      601 agacaaagca aggattgagt gcgtaaaacg tgatgcgtaa tacgtaattc gtaaatcacg
      661 tttcactctt cacgtattac gcattacggc aaaaaaggac ctaaccatga acaaaatcgc
      721 ccgcttaaac ttcgcccatc ttcccacctc aattgaagaa cttcccaacc tttccaaggc
      781 cctgaatgga ccgcatatct tcgtcaagcg ggatgaccag accgggctgg cattcggtgg
      841 caacaaaacc cgcaaattgg aattcctcat tgccgaagcc cgagaacaag gcgcagattt
      901 gcttatttcg ggcggagcga tgcagtccaa tcactgccgt cagaccgcgg cggcagcggc
      961 acgatatgga tttgaatgta agttagtatt gaccggcgcg aaaccgcagc aagcctctgc
     1021 taattattta ctcgaccaac tcttcggcgc agagatcatc accgtggaag atcgcgcctt
     1081 ccgcgaccag actctgcaaa agacgtttga tgacgcgctt gccgccggga agaaacccta
     1141 tcttgttccg tatggcggtt ccagtccaac cggcgcgatg ggctacacct ttgcgatgga
     1201 agagttcatg cagcagaacg cccatgccga ttggatcgtt ttcggtacat cctctggcgg
     1261 gactcatgcc gggctggtgt tgggtcagcg tttgtttggc tataaaggca aagtgttggg
     1321 aatcagcatt gacgagcccg aagaagaact gaagtcacat gtctctgatt tggcttctcg
     1381 cgccagtgaa aaacttggtg agcggattca attctcaagc aacgatgtgc tggcgacaga
     1441 agattattgc cagtcaggct atggcgtctt tggtgaaggc gagcgcgaag caattcactt
     1501 gttcgccaag catgaaggct tattgctcga ccccgtctac accggcagag ccgccgcagg
     1561 gatgatcgac ctgatccgca aaggattttt caagaaagac gagatgatct tattctggca
     1621 taccggcggt cagcccgcgc tgtttgcgga taagtacgcg caagatgtga tacgttaatt
     1681 ttagaccgcg gacggctgac aattgcctgc cgtccacggt ctgctattta ccgtgtgccc
     1741 ttattcacca gcgtcaccac tccaatgatc gcccaaatgg aattgagtgc ggttgccgaa
     1801 tatgcgccgt gactaaaggt gtagtaggtc atgatcacac ctgctagaat gttcatgcct
     1861 tgataggtga atgagtcact ggttactttt ttggcagaat ttaacgcaaa tgccaaaaga
     1921 taagttgccg aaccgatcca gccgagaata tcaattaagt ccattattcc aaaatctcca
     1981 cggtcattcc gatttcaatt tttccctcgt tcaagggaat agtgttcatc ccaaagattg
     2041 cgccgagttc gtggttacga tagccgctca atgttttgag cggttctttg ttttttgcca
     2101 gcgtctcttt atcaatcgtt gtcaccacac agcgcggaca aaccttcact aacgccatct
     2161 ccacactccc gatccgaatg cgcttccatg tatcttctgc gaacggctca cagcctttca
     2221 ctacaaggtt cgggcggaat cgattcatcg gcacagcaga atcaagccgc gagtttaagt
     2281 cctgaagcga ttcttccgag atgaccagaa tcgggtagcc atccgcaaat ccggtgtggt
     2341 catctgcatg gactgcataa agaggattca acttgcgctt gaaaccgtct gcgatatgaa
     2401 cgagtcgcac gcttatacca agccaatccg aaaaccatgt ggcggattca tcaccctgat
     2461 cgatggcatc tacgccgctg ctcgaccaaa tatcgacagg ggttggcgtg ccggaagatt
     2521 gaactgcaaa ctggatcgat tcaaaattcg gcgcggataa agtgaccgcc tcattcctaa
     2581 gggtgggtgt gaccagcgcc agacgcggat gctcgcgctg ggtcagaaac ttgccatcgg
     2641 gcgtcacgac catcatgcgc cgatcatgtt ccagccccat gcgttggact tggctcgcag
     2701 gcacatggaa cccgcggcag gctttgatgg ggtagtaggt gagggtggag agggagatca
     2761 tgggtgtcct tgttgagcga ggtggcgagt ggaaggtag
//
LOCUS       DAOQJE010000484         2793 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_24991_length_2793_cov_4.756392, whole genome shotgun sequence.
ACCESSION   DAOQJE010000484 DAOQJE010000000
VERSION     DAOQJE010000484.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2793)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2793)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2793
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24991_length_2793_cov_4.756392"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..240
                     /locus_tag="PLT08_12195"
     CDS             <1..240
                     /locus_tag="PLT08_12195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE05276.1"
                     /translation="IRRANRILVIEDGRIAEHGTHAELLRARGHYYRLYTQQFRHELE
                     VQYGVAEGETSNEREGVSADSIVVKREKSETVVAD"
     gene            complement(281..2590)
                     /locus_tag="PLT08_12200"
     CDS             complement(281..2590)
                     /locus_tag="PLT08_12200"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNE05277.1"
                     /translation="MEQKIKILFVDDDPEMRTLVSFILKRAGYEVELVESAAEAFQYL
                     THSTPNLILSDVMMADSDGFTLLDTLRKQEATRHIPVILLTALAAPSDLVKGLSLGAD
                     DYIQKPFQSAELLARIQSKINRPPMPSELVPMDIRSGLVKPEQFEEEFKKEFFRSLRN
                     GSEGFIAFLSLSELAITRERVGIDIAPEIWKQAARSIAADLGPTDVIGLANGNLLGII
                     LPDMLEATAHKVLVRLTRKIMNYTFVVGNERFRLTPSVGYANFLSAKNAADARNRALT
                     ALDNAIHHLDMEPRLYEPSMGSLEKREKSNFFQSMAQVLEKLILPFQIGLTFLIGIIF
                     PYFIYRWMDFIGYDITPVAYMIVTISLVLTALLIWVEGFYALRKNPIPETPGAPYPPA
                     TAVIAAYLPNEGATIVDTIEAFLRLDYPAPLQIILAYNSPYEMPIERDLKEIAARDPR
                     FVPFRVSNSTSKAQNINTVIAKVTGEFIGIFDADHHPNADSFTRAWRWLSNGYSIVQG
                     HCAVRNGDSSWVSRLVAVEFEAIYAISHPGRARLHQFGIFGGSNGFWKVDLLRQTRMH
                     GFMLTEDIDSSIRVTMDGVKIASDPDLISRELAPTNLKTLWNQRMRWAQGWHQVSLKH
                     LLQALASKKLTIRQKFGAFWLLGWREAYPWLSMQMFPIILFWIQKYHGVENINWLIPI
                     FVMATLFTLSVGPGQVFFSYRLSLPEIKQHKGWFVFYFIVTSLVYTEFKNTIGRVAQI
                     KELFKERQWKVTPRSAPQPPQGQSRFVSR"
BASE COUNT          756 a          654 c          673 g          710 t
ORIGIN      
        1 atccgccgtg cgaaccgcat ccttgtaatc gaagatggtc gtattgcgga acacggaacc
       61 cacgccgaac tcttacgcgc ccgcggacat tactatcgcc tatataccca gcaattccgc
      121 catgagttgg aagtgcaata cggggtggcg gaaggagaaa caagtaacga gcgtgaaggg
      181 gtaagcgcgg attcgattgt agtgaaacgt gagaagagtg aaactgtggt ggcggattaa
      241 taagtaaaag atgtccctga gagttctcag ggacatcttt ttatctactc acgaatcgac
      301 tttgaccttg cggcggctga ggagccgagc gcggggtgac cttccactgg cgctctttga
      361 ataactcctt gatctgtgct acccgaccaa tggtattttt gaattccgta tataccaatg
      421 aagtaacgat aaagtaaaag acgaaccaac ctttatgttg cttgatttca ggcaggctga
      481 gccgatacga gaagaaaacc tgacccggtc ccacgctcaa tgtaaataga gtcgccatca
      541 cgaagatggg aatcaaccag ttaatgtttt ccacgccatg gtatttctgt atccaaaaca
      601 ggatgatcgg gaacatctgc atcgaaagcc aggggtaggc ttcacgccaa cccaacaacc
      661 agaaggcgcc gaacttttgt cgtatggtta gctttttaga tgccagcgcc tgcaatagat
      721 gtttcaagga aacttggtgc cagccttgag cccaacgcat acgttgattc caaagagttt
      781 tcaggttggt gggtgccagt tcacgcgaga ttaagtctgg gtcggaggcg atcttcactc
      841 catccatcgt gacgcggatt gaagagtcga tgtcttcagt tagcataaaa ccatgcatgc
      901 gagtctggcg cagcaggtca accttccaaa atccgttcga gccgccgaag ataccaaatt
      961 gatgcaaacg ggcacgtccc gggtgactga tcgcatagat cgcctcaaac tccacggcga
     1021 caaggcgcga cacccatgaa gaatcaccat tgcggacagc gcaatgcccc tgcacaatgc
     1081 tgtatccgtt cgaaagccag cgccatgcgc gggtaaaact atcagcattc ggatggtggt
     1141 ctgcatcaaa gatacctata aactcgccag taaccttggc aattacggta ttgatatttt
     1201 gagccttgga tgtactattg ctgacccgga acggtacaaa acgcggatcg cgtgcggcaa
     1261 tttctttcaa gtccctttcg atgggcatct cgtatggact gttataagca aggatgatct
     1321 gcaaaggcgc aggatagtcc aacctcaaaa acgcttctat tgtgtcgaca atggttgcgc
     1381 cctcgttggg caaataggca gcaatgacag cggtcgctgg cgggtatggc gccccaggcg
     1441 tttcagggat tggattcttt ctcagcgcgt aaaatccttc cacccagatc agaagcgctg
     1501 taagcaccaa cgaaatggtc acgatcatat aggctacagg ggtaatgtcg taaccgataa
     1561 agtccatcca acgatagatg aagtacggga agataatgcc gattagaaag gttagcccaa
     1621 tttgaaaggg taggatcagt ttctccaaga cttgcgccat agactgaaag aagttgcttt
     1681 tctctctctt ttcaagagac cccatagacg gttcgtaaag gcgcggctcc atatccaagt
     1741 ggtggatggc attgtccaaa gcagttaagg cgcggttcct agcgtcggct gcattttttg
     1801 cagaaagaaa atttgcatat ccaaccgaag gcgttagacg aaatcgctca ttcccaacta
     1861 caaatgtata gttcatgatc tttcgcgtga gcctgactaa aactttatgc gcagtggctt
     1921 ccagcatatc cggcaggatg atccccaaga gattcccatt tgccagaccg atcacatccg
     1981 taggacccag gtctgcggcg atggatcgag ctgcctgctt ccatatctca ggtgcgatat
     2041 ctatgccgac gcgttcgcga gtaatggcga gttcggataa agataaaaac gcgataaacc
     2101 cttcagagcc attgcgtagg gatcggaaaa actccttttt aaattcttct tcgaattgtt
     2161 ctggtttcac caacccactg cggatgtcca tgggcaccaa ctcgctaggc atgggcggac
     2221 gattgatctt cgattgaatt cttgccaata gttcagccga ttggaatggt ttctgtatgt
     2281 aatcatctgc gcccaaggac aaccctttta ctaggtcact gggggcagcg agagcagtaa
     2341 gtaatatcac tggaatatgt ctggttgcct cttgttttct gagtgtatca agcagagtaa
     2401 aaccatcgga atctgccatc atgacatctg acaaaataag gttgggggtt gaatgggtta
     2461 gatactgaaa tgcctcagcc gcactttcga ccaactccac ttcgtagcct gctcttttga
     2521 gaatgaagct tacaagggtt cgcatttccg gatcatcgtc tacaaatagg atttttattt
     2581 tctgttccat tttttagctc cttgaaagac aatctttcaa tttcgcttag aaaatacccc
     2641 aaaactatgt attaattcca ttagggaaaa cctgtatgaa acaaaaaaga caaaaccctc
     2701 tatccatttt gtaaggaaga gggttttgtc tttgcaattt ttttgtttaa ttatttaatt
     2761 cttcacatgt tctcggagct gatacggaac att
//
LOCUS       DAOQJE010000485         2792 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_25007_length_2792_cov_5.448666, whole genome shotgun sequence.
ACCESSION   DAOQJE010000485 DAOQJE010000000
VERSION     DAOQJE010000485.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2792)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2792)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2792
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25007_length_2792_cov_5.448666"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..435
                     /locus_tag="PLT08_12205"
     CDS             <1..435
                     /locus_tag="PLT08_12205"
                     /EC_number="1.14.19.-"
                     /inference="COORDINATES: protein motif:HMM:NF012699.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid desaturase"
                     /protein_id="HNE05278.1"
                     /translation="WTIHLIGWQAYLLVELPILLMACGAGVWLFYVQHNFDPTYWERH
                     KDWSFFNAGMDGSSFYKLPKVLQWFTGNIGFHHIHHLSPKIPNYKLEECHNENPIFQI
                     EPLTFRDSLKSLYFRLWDEKEKVLVGWNAIKKYKMQTQRNQM"
     gene            complement(489..1949)
                     /locus_tag="PLT08_12210"
     CDS             complement(489..1949)
                     /locus_tag="PLT08_12210"
                     /inference="COORDINATES: protein motif:HMM:NF013056.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="solute carrier family 23 protein"
                     /protein_id="HNE05279.1"
                     /translation="MATKNSKTKPKAPTKSAAAKQVEVQKEAANPKVFGYLPDDNPPI
                     AAMLSLGFQQFLTMFPATVLVAILTKFDVGVTLLASGLGTIIALLVSKRKIPMYYGSS
                     FSYIAVIITVMSQFAPDCFNDATQVYCADGVKIVQVGIILTAVFEILVGLLIMRVGKS
                     ALDRVLPPIVTGSMAMVIGIALANAALGNAGNWAAPELATATWTVAFLTLLATVLFSV
                     YLQGKGLIGMLPILLGAIFGYLVSIPFGLVTFDKVANAAWLEMPKFTFPAFTNPAAWG
                     MALSVALIFIATVPESTAHLYQMSLYIDELAAELGRKPFNIKELIGLNLVADGADDLV
                     VGFLGGAPGTNYGENNSLMAITRNYSTAVLMTAGAMAIVLGFFGKLVALVNTIPAAVV
                     GGLSIYLFGVIGMQGVALIQSEKVNLFDPKQLAVGAIILICGIGGNLSLPNGVFPFPI
                     PYLFPSGIPAIVFAAIAGILVNTIFVLFKAPEVRGG"
     gene            2279..>2792
                     /locus_tag="PLT08_12215"
     CDS             2279..>2792
                     /locus_tag="PLT08_12215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate/isopropylmalate family dehydrogenase"
                     /protein_id="HNE05280.1"
                     /translation="MAYENVKVPEGGAKISIQDGKLNVPNNPIIPFVEGDGTGRDIWR
                     ASVRVFDAAVEKAYGGKRKINWMEVYAGEKSFKLFQSWLPDETTEAFKEFLVGIKGPL
                     TTPIGGGIRSLNVQLRKVLDLYVCLRPVRWYKGVPSPVNHPENVDMVIFRENTEDIYA
                     GIEFANGTEDN"
BASE COUNT          736 a          697 c          752 g          607 t
ORIGIN      
        1 tggacgatcc atctgatcgg ttggcaggca tatttgctgg tggaacttcc cattttgctc
       61 atggcgtgcg gcgcgggtgt gtggctgttc tacgtgcagc ataacttcga ccccacctac
      121 tgggaacgcc acaaagattg gagcttcttc aatgctggca tggatggcag ttccttctat
      181 aagctcccca aggttttgca gtggttcacg ggcaatatcg gcttccatca cattcatcac
      241 ctcagcccga agatccccaa ctacaagtta gaggaatgcc acaacgagaa tcccatcttc
      301 cagatcgagc cgctcacctt ccgcgacagt ttgaagtcgt tgtacttccg cctgtgggat
      361 gagaaagaga aagtgctggt cggctggaat gcgatcaaga aatacaagat gcaaacgcaa
      421 agaaaccaaa tgtagttaaa caaaaagtga ctatcaaaac gacagtcact ttttttgttt
      481 caaacggatt atccgccgcg tacttcaggg gctttgaaca agacaaagat ggtgttcaca
      541 agaataccgg caatcgcagc aaagacaatg gcagggatgc cactggggaa aagatacgga
      601 attgggaagg ggaacacccc attgggcagc gagagattcc caccgatgcc gcagatcaag
      661 atgatcgcac caaccgcaag ctgtttcggg tcgaagagat tgaccttctc cgattgaatg
      721 agtgccacgc cttgcatacc gatcacaccg aagagataga tgctcaagcc gccgacgact
      781 gccgccggaa tggtgttgac caacgccacc agtttcccga agaagccaag cacaattgcc
      841 attgctcctg cagtcatcaa tacggcagtg gagtagttgc gagtgatcgc catcaacgaa
      901 ttgttttcgc cgtagtttgt accgggagcg ccgccgagga agccgaccac gagatcatcc
      961 gcaccgtcgg caacgaggtt caaaccgatg agttctttga tgttgaaggg tttgcgaccc
     1021 aattcagcgg cgagttcgtc gatgtacaaa ctcatctgat acagatgcgc cgtcgattca
     1081 gggacggtag ctatgaagat cagtgccacg ctcaatgcca tgccccaagc agcggggttg
     1141 gtaaatgccg ggaaggtgaa cttgggcatt tcgagccagg cagcatttgc cactttatca
     1201 aaggtcacca acccaaacgg aatggaaacc aaatatccaa agatcgcgcc cagcaaaatg
     1261 ggcaacatgc cgatcagccc tttgccttgc aggtagaccg agaacaacac cgtggcgagc
     1321 aaggttagga aggcaactgt ccatgtggcg gtggcgagct caggggctgc ccaattgccg
     1381 gcattgccga gcgccgcatt tgccagggcg ataccgataa ccattgccat cgaaccggtc
     1441 acaataggcg ggaggacgcg gtcaagtgcg cttttaccta cacgcattat tagcaaaccg
     1501 accagaattt cgaacactgc cgtcaaaatg atgcccactt gcacgatctt cacgccatcg
     1561 gcgcaataga cttgggtcgc atcgttgaaa caatcaggcg cgaactggct catcaccgtg
     1621 atgatgacgg cgatgtatga gaacgacgag ccgtaataca tcgggatctt gcgcttcgaa
     1681 accagcagcg caatgatcgt ccccaagccc gaagccagca acgtcacccc cacatcgaac
     1741 ttggtgagga tcgccaccaa cacggttgcc gggaacatcg tcaggaattg ctggaagccc
     1801 aggctgagca ttgccgcgat cggcggattg tcatcgggca gatagccgaa gactttggga
     1861 ttcgccgcct ctttctggac ctcaacctgt ttcgcagccg cagacttggt tggtgccttt
     1921 ggcttggtct tgctattttt tgttgccatg tgcattctcc tcgatctgag ataaaaaaag
     1981 agagccgcta caagcgactc tccgattcac ataatagaga cagccattcc aaagaagtgt
     2041 tgatgcggga aaggatgtcg aaatttttct gttaacattt tggcgaattt tacgcctatt
     2101 taagagagtg tcaaggaatt tgagcgcttt gggcgtggaa aatttttcag aaaaccgaag
     2161 caactgcgag cggggaaagg atgcgctgat taagagcatc tccacttatg atggttaagt
     2221 cgtgggtgta taatccagcc gatcattttc aaatatataa aagtcaaaag aggtagacat
     2281 ggcatacgag aacgtgaaag tccccgaagg cggggcaaag atttcaattc aggacgggaa
     2341 gttgaacgtc cctaacaatc cgatcatccc gttcgtggaa ggcgacggaa ccgggcgcga
     2401 tatttggcgc gcttcggtgc gtgtgttcga tgcggcggtg gagaaggcgt acggcggaaa
     2461 acgcaaaatt aactggatgg aagtgtacgc gggcgaaaag tcattcaagt tgttccagag
     2521 ctggttgccc gatgaaacca ccgaagcctt caaggaattt ttggttggta tcaagggacc
     2581 gctgaccaca cccattggcg gcggtatccg ctcgttgaat gtgcagttgc gcaaggtgct
     2641 cgacctgtac gtttgtctgc gcccggtgcg ctggtataag ggcgtgccct cgccagtgaa
     2701 tcaccccgaa aatgtggata tggtcatttt ccgtgaaaac accgaagaca tttatgccgg
     2761 tatcgaattt gcgaacggca ccgaagataa ca
//
LOCUS       DAOQJE010000486         2772 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_25328_length_2772_cov_5.923445, whole genome shotgun sequence.
ACCESSION   DAOQJE010000486 DAOQJE010000000
VERSION     DAOQJE010000486.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2772)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2772)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2772
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25328_length_2772_cov_5.923445"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..206
                     /locus_tag="PLT08_12220"
     CDS             <1..206
                     /locus_tag="PLT08_12220"
                     /inference="COORDINATES: protein motif:HMM:NF017880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF952 domain-containing protein"
                     /protein_id="HNE05281.1"
                     /translation="LVLLVVDESRLKPELKWEAPAGPPAENISEADLFPHLYGPLNLD
                     AVASVLDFQPDSAAGTFSLPKLG"
     gene            226..2685
                     /locus_tag="PLT08_12225"
     CDS             226..2685
                     /locus_tag="PLT08_12225"
                     /inference="COORDINATES: protein motif:HMM:TIGR03662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2298 domain-containing protein"
                     /protein_id="HNE05282.1"
                     /translation="MTAFLPWYLILTLLGWLTFPLVYHLFPMLTDRGFTLARTAGLLI
                     WGYVFWLFVSFGFARNDIGGIVFALTILIALSVWSFFVHYTSILAFVRSKWTLIVTTE
                     VLFLIAFAFIAFVRAADSELASTEKPMELMFINSILRSETFPPQDAWLSGYAISYYYF
                     GYVLTAMLAQLSKINGSTAHNLMLSLVFALGAIGSFGIVYNLLSTFHSKKRSDEQGSI
                     SSVVSALLAPLFLLLVSNFEALLEVLHRAGIGWTKDAATQTWSGPFWTWLDMKELSQP
                     PVEPFGWVPDRYLWWWRASRVIQDYDIVGGFREAIDEFPFFSFLLGDLHPHVLAIPFG
                     LLAVSVALNIFLGGWKNGKVNFLGAQIHILPTGFLFAALVLGGLAFLNTWDILVAAAL
                     IVFAFVLWRANEEGWGWARLEDMLLLGLPLGILSLLLYFPFYIGFSSQAGGLLPNLMY
                     IARGTHLWVMWGTLLIPIYLFLITVLMKNPARPNWKLGFSIGLGTPLLLFLLAVILSL
                     LALIVEKDFTNALLASYNMTTGQFFAATSMRRLTHIGSLLTLVAVLIPAVAFVFSQNG
                     EQEVNEKEESATTSSSLQFLFLLLTLGALLILAPEFVYLRDQFGYRINTIFKFYYQAW
                     ILWSLVAAFGIGYVLQSLRGVADITARILVGAVIFCGLLYPTFGIMTKTNNLKPVFGF
                     NLNDFARVQRENADDAAGIEFLLTQPEGVIAEAIGGSYSYYGRVATHTGYPTVLGWPG
                     HESQWRGGSELQGTRQDDINLLYSTVRWEEAQAILEKYSIRYVFIGNLERTSMRVSEE
                     KFAAHLTPVFQQGITVIYAAP"
BASE COUNT          546 a          702 c          649 g          875 t
ORIGIN      
        1 atttggtctt gctggtggtt gatgaaagca gactcaagcc cgagcttaag tgggaagcgc
       61 cagcgggtcc gcctgctgaa aatatttctg aggcagattt atttccgcat ctgtatggtc
      121 cgttgaattt ggatgctgtc gcctctgttc tggactttca gcctgattct gctgccggaa
      181 cgttttctct cccgaagttg ggttaaccca ctgagtactg actagatgac cgcttttctc
      241 ccttggtatc tcatccttac attgcttggc tggctgacct ttccgctggt ctatcatctt
      301 ttccccatgc ttaccgaccg cggctttacc ctcgcacgga cagccggttt gctgatctgg
      361 ggatatgttt tttggctatt tgtttctttt ggttttgcgc gcaatgatat tggtgggatc
      421 gtttttgcgt tgacgatctt gatcgcttta agcgtttggt ccttttttgt tcattacacg
      481 tccattcttg cttttgtgcg ctccaagtgg acgttgatcg tcaccacaga agttctgttt
      541 ctgatcgcct ttgcctttat tgccttcgtc cgcgccgcag actctgaact tgccagcact
      601 gaaaaaccaa tggaattgat gttcatcaat tccattttgc gttcagaaac cttcccaccg
      661 caagatgcct ggctttcagg gtatgccatt tcgtattatt acttcggcta tgtgttgacc
      721 gccatgctgg ctcagctttc caagatcaac ggcagtacag cacataacct gatgctttca
      781 ctggtcttcg ctcttggtgc aattggttcc tttggtatcg tatataacct gctaagtacc
      841 ttccacagca aaaagcgatc tgatgaacaa ggctcaatat cttcagttgt atcagcgtta
      901 ttagctccgc ttttcctttt gctcgtttca aattttgaag ccttgcttga ggtgctacac
      961 cgtgcaggca tcggctggac aaaagatgcc gccacccaaa catggagcgg ccctttctgg
     1021 acatggctag acatgaaaga actgtctcag ccgccggttg aaccatttgg ctgggtacca
     1081 gatcgttatc tatggtggtg gcgtgcttca cgcgtcattc aagattacga catcgttggc
     1141 ggcttccgcg aagccatcga cgaatttccc ttcttctctt tcctgcttgg cgatcttcat
     1201 ccgcatgtgt tggcaattcc atttggcttg ctggctgttt ctgtggcgtt gaacatcttc
     1261 ctcggcgggt ggaagaatgg caaagtcaac ttcttgggcg cgcaaatcca tatcttgcca
     1321 acaggttttc tgtttgccgc ccttgtactt ggcgggctcg ccttcctcaa cacttgggat
     1381 attcttgttg ctgcagcgtt gatcgtcttt gccttcgtcc tttggcgtgc caatgaagaa
     1441 ggttggggct gggcacgcct cgaagatatg cttttgttag gcttaccgct cggaatcttg
     1501 tctctccttt tatattttcc gttttacatt ggcttctctt cccaagcggg cgggttgctc
     1561 cccaacctca tgtatattgc ccgtggcacc catctttggg tgatgtgggg cacgctgttg
     1621 attccgattt atctttttct tattactgtt ttgatgaaga acccggctcg cccaaactgg
     1681 aagttgggtt tctccattgg gttgggcacg cctctgttgc ttttcctact cgcggttatt
     1741 ttgagcttat tggcattgat cgttgaaaaa gatttcacca atgctttgct tgcttcctac
     1801 aacatgacca ccgggcagtt cttcgccgcc acctccatgc gcagactgac ccatattggc
     1861 agtttgctca ccttggtcgc tgtgttaatc cctgccgtgg catttgtctt ttctcaaaat
     1921 ggtgaacaag aagtaaacga gaaagaagaa tctgcaacta cctcttcttc ccttcaattt
     1981 cttttcctac tgcttaccct tggtgcgctg ctcattcttg cgcccgagtt tgtttattta
     2041 cgagaccaat ttggatatcg catcaacact atcttcaagt tctactacca agcgtggatt
     2101 ctctggagct tagtcgcagc ctttggcatt ggttatgtcc ttcagagcct gcgcggagtt
     2161 gcggatatta cggcgcgcat tttggtggga gcggtcatct tttgtgggtt gctctaccca
     2221 acctttggca tcatgaccaa gaccaataat cttaagccgg tctttggttt caacctcaat
     2281 gattttgccc gcgtacaacg tgaaaatgct gatgacgccg ccgggatcga atttttgctc
     2341 actcaacctg agggtgtgat cgccgaagcc attggcggaa gttatagcta ctatgggcgt
     2401 gtggcaacgc atacaggtta ccccacagtt ttgggttggc caggtcatga gtcacagtgg
     2461 cggggcggtt cagaactgca aggcacacgg caagacgata taaacttgct atattccact
     2521 gtccgttggg aagaagcaca agccattctc gaaaaatata gtattcgtta tgtcttcatc
     2581 gggaatttgg agcgcacatc catgcgtgtg agcgaagaaa agtttgccgc tcacctcact
     2641 ccagttttcc agcaaggcat tacagtcatt tatgcagccc cataataaaa gagccctgat
     2701 cattcagggc tcttttattt ctactgtaca acgcctgttg cgtctacggg atatcccttg
     2761 tcgtaccatg cc
//
LOCUS       DAOQJE010000487         2762 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_25486_length_2762_cov_6.375693, whole genome shotgun sequence.
ACCESSION   DAOQJE010000487 DAOQJE010000000
VERSION     DAOQJE010000487.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2762)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2762)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2762
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25486_length_2762_cov_6.375693"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1166
                     /locus_tag="PLT08_12230"
     CDS             <1..1166
                     /locus_tag="PLT08_12230"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNE05283.1"
                     /translation="ALGTWGFLGLFDKEDRLSWLPTSFDIVQTTANLLGAAIERMHYE
                     ETLRLSERRNRIIVDALPDLLLRVDRSGRIFDYNATSSHPLYLEPASAIGKNLYELWN
                     DDIAGKLLPRLPDETLFNLPRTVENFKLPGKLGSFEARLFPLSKLEALIIVRDITEQA
                     RLNQMKSDFINWASHELRTPLTSAILMTELLRQECTPEEAEEYLSTLSSELNRQKNLI
                     NELLMAGRLENGTLHVEVTAVDLHPVLKDAILSVKPIARKRNIAIQMVTAANPCFVMG
                     NVSALQQVFINLLSNAVKFSPDDSQVILRVDRKDTETHVAVVDSGLGIPSDAMPYLFE
                     RFYRARNVSAAEIPGSGIGLFIVKSILKELNGSISVDSKLNEGTTFTVVLKNK"
     gene            complement(1198..2388)
                     /locus_tag="PLT08_12235"
     CDS             complement(1198..2388)
                     /locus_tag="PLT08_12235"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HNE05284.1"
                     /translation="MVQSLIALIKQLFIPPVFEGDEEKTRRASVLNFGVNIILFASPL
                     IILGNLIGGKIPPPVSAINLLVIVASLILRHLLHRGRVLSASIVFMSIGFSLNTLGII
                     GLGTIRTPTTATYLFFIVMAVILFDYKGVIASISICSITILGLIMAENNGILPPPNLS
                     VTVTQWVSYTVLFTLTAAISNEANRMMKSALHKAENENAERKRVEIELRRVNDLLVLQ
                     LEANNSLQLELREQALRDPLTGLYNRRYLRETMEQEVKKAKRDKTPLSVIVSDIDHFK
                     LINDSYGHQVGDRFLVEIATLMKNHARSSDFVCRYGGEEFLIIMPGTPASSAIKRAEE
                     ILQKCTDTVVVHEGNNLQVAMSLGVATYPDHGQEAEELIIKADKALYVSKHNGRNRIT
                     LSNV"
     gene            2467..>2762
                     /locus_tag="PLT08_12240"
     CDS             2467..>2762
                     /locus_tag="PLT08_12240"
                     /inference="COORDINATES: protein motif:HMM:TIGR00149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secondary thiamine-phosphate synthase enzyme
                     YjbQ"
                     /protein_id="HNE05285.1"
                     /translation="MNWYKTTLEISTHGKGLHDITPSIRAQLKEWNAREGMCFLFLQH
                     TSASLVISENWDPTARADLETFMERLVPERDSWYTHDLEGPDDATSHIRSMLTDT"
BASE COUNT          747 a          585 c          646 g          784 t
ORIGIN      
        1 gggcattggg aacatgggga tttttaggct tgttcgataa agaagatcgc ctctcttggt
       61 tgcccaccag ctttgatatt gtgcagacga ctgctaattt gctgggtgct gcaattgagc
      121 gtatgcatta cgaagagact ttgcgtttga gtgagaggcg taaccgcatc atcgtggacg
      181 cgttgcctga tctgcttctg cgagtggatc gttcgggacg aatttttgac tataacgcaa
      241 cctcttcgca cccgttgtat cttgagcctg ccagtgccat tggcaagaat ttgtacgaat
      301 tgtggaatga cgatattgcg ggaaagttgt taccgcgcct tcctgatgag acgttgttta
      361 atttgccacg tactgtggaa aattttaagt tgccgggaaa attgggctct tttgaagcgc
      421 gcttgtttcc tctcagtaaa ctagaagcgt tgattattgt gcgcgatatt acagaacagg
      481 cacggcttaa tcagatgaaa tctgatttta ttaactgggc atcacacgaa ttacgtaccc
      541 cattaacttc tgctatctta atgacagaac tgcttagaca ggaatgcacg cccgaagaag
      601 cagaagaata tctttctacg ctgtctagtg aactgaaccg acagaagaat ttgatcaatg
      661 aattgttgat ggctggacga ttggaaaatg gaaccttgca tgttgaggtt accgcagtag
      721 acctgcaccc agtattgaaa gatgccattt tatcggtcaa accgattgca agaaagagaa
      781 acattgccat tcaaatggtg acggctgcca acccttgttt tgtcatggga aatgtaagtg
      841 cgcttcagca ggtgtttatc aatttactaa gcaacgccgt gaaattctct ccggatgata
      901 gtcaggtgat tttgcgagtg gatcgcaaag atacagaaac acatgtggca gttgtagata
      961 gtgggttggg tatcccttct gatgcaatgc cctatctttt tgagcgtttt tatcgtgccc
     1021 gtaacgtaag cgccgcagag atccctggca gtggaattgg tttgttcatc gtaaaatcta
     1081 ttttgaaaga gttgaatggt tcaatttcag tagacagtaa attgaacgag gggacaacct
     1141 tcacggttgt tttgaaaaat aaataaaatc ggatgagatg ctcatccgat ttttgtttta
     1201 tacattcgag agagtgattc tatttctgcc attgtgtttg gatacataca aagctttatc
     1261 tgccttgatg ataagttctt cggcttcttg tccatgatcg ggataagtgg caacgcctaa
     1321 tgacatagcg acttgtaaat tattgccttc atgtacaaca accgtgtccg tgcatttttg
     1381 cagaatctct tctgctcgtt ttatggcaga gctggcaggc gtgcccggca taatgattag
     1441 aaattcttcc ccaccatatc ggcagacaaa atctgaactc cgtgcatggt ttttcatcag
     1501 tgttgcgatt tcaaccagaa accggtcacc aacctgatga ccgtatgagt cgttgatcaa
     1561 tttaaagtgg tcaatatcag agacgatgac acttagcggg gttttgtcgc gttttgcttt
     1621 ttttacttct tgttccatgg tttcgcgaag ataacgccgg ttgtatagcc cagttaatgg
     1681 gtcacgcaga gcttgttcac gcaattcgag ttgcaggcta ttgttcgcct ccagttgaag
     1741 cactaaaagg tcattaaccc tacgcaattc tatttctaca cgtttccgtt cggcattttc
     1801 gttttcggct ttgtgcaacg cacttttcat catccgattt gcttcatttg agattgcagc
     1861 agttagtgtg aacagaaccg tgtacgaaac ccactgagtg acggtaacag aaagattggg
     1921 cggcgggaga ataccgttat tttccgccat gattaatccc agaatcgtta tggagcaaat
     1981 gcttattgag gcaataaccc ctttatagtc aaatagaatg actgccataa caataaaaaa
     2041 taagtaagta gctgtagtag gtgtgcgaat ggttccgagc ccgataattc ccaaagtgtt
     2101 cagcgagaaa ccaatcgaca taaatacaat actagcagag agaactctgc cacgatgtag
     2161 caaatgacgt aatatcaaac tagctacaat gaccagcaaa ttgatcgccg aaaccggagg
     2221 cggaattttt cctccgatca ggttgccaag gatgatgagc ggagaggcaa aaaggatgat
     2281 gtttacaccg aaattaagca cgcttgcgcg acgcgttttt tcctcatctc cttcaaaaac
     2341 cggcggaatg aataactgct tgatcagtgc tattagtgac tggaccatat tttgagtagc
     2401 cgaagtatat cgtacgcgga ctggttttcc taaagtttga tagacccacc tgctataatt
     2461 tcggacatga attggtataa aactacactt gaaatttcca cccatggcaa aggacttcac
     2521 gatattacgc caagcatccg tgcccagcta aaagaatgga atgcgcgtga gggaatgtgc
     2581 tttttattcc tccaacatac cagtgcttca ctggtgatca gcgagaactg ggatccaact
     2641 gctcgagcag atttggagac cttcatggaa cgcctcgttc ccgaacggga cagttggtac
     2701 acccacgatc tggaaggacc cgacgacgcc acctcccaca tccgctcgat gttgaccgat
     2761 ac
//
LOCUS       DAOQJE010000488         2757 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_25569_length_2757_cov_7.221688, whole genome shotgun sequence.
ACCESSION   DAOQJE010000488 DAOQJE010000000
VERSION     DAOQJE010000488.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2757)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2757)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2757
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25569_length_2757_cov_7.221688"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..660
                     /locus_tag="PLT08_12245"
     CDS             <1..660
                     /locus_tag="PLT08_12245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE05286.1"
                     /translation="GQHPPALIPKMVSLIEQGYDVVQGQRLEEERAASFKKVTSNFFY
                     WLINRISGTQVLQGAADFRALSRNALNGVRSMHEYHRFLRGMISWMGYNSVILPYHEP
                     DRIAGKSKYSLGKMVRLASDAIFSFSLAPLYIGLSAGFVFFILAGVQLIYVLSLWLTG
                     NTQRVEPGWSSLMGVLLIASGIIMILLGFIGVYVGYIFQEVKRRPIYLVKGRTPDARD
                     E"
     gene            644..1291
                     /locus_tag="PLT08_12250"
     CDS             644..1291
                     /locus_tag="PLT08_12250"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF012521.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HNE05287.1"
                     /translation="MPETNKPKIFNITLLRHGESVGNAEARWQGQSDFPLTERGRKQA
                     QALAERWKREKMKFDFVISSPLSRARDTAEIIAAKLGLTVDFEPLWLERDNGEFAGLT
                     QHEVRNNFSHPDFTTPYDAVGNNGEGDWELFLRAGQALHDLLKREPARYLIVSHGGLL
                     NQVMHAVVGIVPQANNAGTRFRFSNTAFAQLLYFPHQHRWAIDKLNDHIHWLDAE"
     gene            1379..2461
                     /locus_tag="PLT08_12255"
     CDS             1379..2461
                     /locus_tag="PLT08_12255"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008677 - 2-dehydropantoate 2-reductase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HNE05288.1"
                     /translation="MSLPSSPSSELKFLVFGAGAIGTYIGGSLVLAGHQVVFVEKPKM
                     VEELKAKGLRLDLTVDERRNTKDVSIIDSKSFIIEPSLEDALKYGPFDAALFALKSFD
                     TVSAMEGLKPFADKLPPLLCLSNGVDNEPIIARTLGPDKVIYGTVTTAIGRRGPGSIV
                     LEKLRGVGIAAGHPLSEKLVAAFDNAYLKCRLYPDALSMKWSKMLTNLVANPTSAILN
                     MTAAEVFADKRLYKIEIDMLKECLEVMASMNLEVTDLPGTPVRALAFATKLPLWLSKP
                     FLSKAAGSGRGGKMPSFHIDLYSGRPQSEVEFLHGAVVREGKTRGIPTPVNKVLTETL
                     LALTNKEILLEEFAGKPEKLLSKLRY"
     gene            2510..>2757
                     /locus_tag="PLT08_12260"
     CDS             2510..>2757
                     /locus_tag="PLT08_12260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside kinase"
                     /protein_id="HNE05289.1"
                     /translation="MIQITEPTPNVEVHLPNGKTLTGPRGTKVGEFLAQVRDDFPAPI
                     VAAIINNQIHELTYPIMVESACIPVTMDTADGARIYRRS"
BASE COUNT          676 a          647 c          682 g          752 t
ORIGIN      
        1 ggacaacacc cgcctgcttt gattcccaag atggtcagtt tgatcgaaca gggctacgat
       61 gtggttcaag ggcagcgtct cgaagaagaa cgtgcggctt cgttcaagaa agtcacttct
      121 aactttttct attggcttat caaccgcatc agcggtacac aggttttaca aggggctgca
      181 gattttcgcg ccttgtcgcg taatgcattg aacggcgttc gttcgatgca cgaatatcat
      241 cgcttcttgc gcggcatgat ttcatggatg gggtacaaca gcgtcatcct gccgtatcat
      301 gagccagacc gtattgctgg gaaatcaaag tattcacttg gcaaaatggt gcgcttggct
      361 tcagatgcga tcttttcgtt ctcgcttgcg ccgctgtata ttggtttaag tgctggcttt
      421 gtctttttta ttttagcggg cgtacagttg atttacgtcc tctctttgtg gcttaccggc
      481 aatacgcaac gcgtggaacc cggctggagt tcattgatgg gcgtgctgct gattgccagc
      541 ggcattatta tgatcttgct gggttttatt ggcgtgtatg ttggctatat ctttcaggaa
      601 gtgaagcgcc gcccaattta tcttgtgaag gggagaactc cagatgcccg agacgaataa
      661 accaaagata ttcaatatta ctttgctgcg tcatggcgag tcagtgggga atgctgaggc
      721 gcgctggcag ggacaatcag atttcccgct taccgaacgt ggacggaagc aagcgcaagc
      781 cttagccgag cgttggaagc gcgaaaagat gaaatttgat ttcgtcatct ccagtccgct
      841 ttcgagagcg cgcgatacag cagagatcat cgctgccaaa ctcgggctga ccgttgattt
      901 tgagccgcta tggttagagc gcgataacgg cgaatttgcc gggctgacac agcatgaagt
      961 gcgcaataac ttttctcatc ctgatttcac cacgccttac gatgcagtgg ggaataatgg
     1021 agaaggtgac tgggaactct tcctacgagc gggacaggcg ttgcatgatt tgttgaagcg
     1081 tgagccggct cgttatctaa tcgtttctca tggtgggttg ttgaatcagg tcatgcatgc
     1141 agtggtgggg atcgtcccac aagctaataa tgcggggacg cgctttcgct ttagtaatac
     1201 cgcttttgcc caactgttat attttccaca ccaacatcgc tgggcaattg ataaactcaa
     1261 tgaccacatc cattggcttg acgccgaata gtttgtaact ggtaaagagt gaatgcgatt
     1321 tgcatatcac gttcactctt atctatttat cctttaacac ttggaatcaa aaataaccat
     1381 gtctctccct tcttctccat cttctgaact taaatttctc gtctttggcg caggcgccat
     1441 tggtacgtat atcggtggtt cgcttgtttt ggcgggacat caagttgttt tcgtagaaaa
     1501 gccaaagatg gtggaagaat taaaagccaa aggtctgcgg ctggacctaa ccgtggacga
     1561 aaggcggaat acgaaagatg taagtattat tgattcaaag tctttcatca tcgagccttc
     1621 gttggaagat gctttgaaat acggtccttt cgatgcggcg ctctttgcat tgaaatcatt
     1681 tgataccgtt tctgcaatgg aaggcttgaa gcccttcgca gataaactcc cgcctttgct
     1741 ttgtctctcc aatggtgtgg ataacgagcc tatcattgcc cgtactctcg gacccgataa
     1801 agttatttac ggaacggtca caactgccat tggcaggcgt ggacctggca gcattgtcct
     1861 tgagaaattg cgtggcgtgg gcatcgcagc gggacacccc ctctcagaaa agcttgttgc
     1921 tgcgtttgat aatgcatatc ttaagtgccg cttgtatcct gatgcattaa gcatgaaatg
     1981 gtcgaagatg ctgaccaatt tggtggcgaa tcccacatct gccattctca acatgacggc
     2041 agcagaagtc tttgccgata agcgcctata caagattgag atcgatatgc tcaaggaatg
     2101 tttggaggtg atggcatcca tgaacctcga agtgactgat ttgcccggaa cacccgttcg
     2161 agctttggca tttgcaacca aacttccgct ttggctctcc aaacctttcc tatccaaagc
     2221 cgcaggcagc ggacgcggcg gtaagatgcc atctttccat attgacctgt attcgggtcg
     2281 cccgcaaagc gaagtggaat ttttacatgg agccgtcgtc cgcgaaggga agacacgtgg
     2341 aattccaact cctgtcaata aagtactcac tgaaaccttg ctcgcactca caaataaaga
     2401 aattctgctc gaagaatttg caggcaaacc agagaaattg ttatcaaaat taagatacta
     2461 ggaacttgtc aaaggtcatc gcaagcagac ctttgacatt tgatcttcca tgattcaaat
     2521 caccgaacca actccaaacg tcgaagtcca tttgcctaat ggcaaaacct taaccggtcc
     2581 acgagggacg aaagtggggg agttccttgc acaggtccgc gatgatttcc ctgcgccgat
     2641 tgtggctgcc atcatcaata accagattca tgaattgacc tatcccatca tggtcgaatc
     2701 agcttgtatt cctgtcacta tggatacagc cgatggtgcc cgcatttacc ggcgttc
//
LOCUS       DAOQJE010000489         2757 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_25577_length_2757_cov_3.634345, whole genome shotgun sequence.
ACCESSION   DAOQJE010000489 DAOQJE010000000
VERSION     DAOQJE010000489.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2757)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2757)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2757
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25577_length_2757_cov_3.634345"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..714)
                     /locus_tag="PLT08_12265"
     CDS             complement(<1..714)
                     /locus_tag="PLT08_12265"
                     /inference="COORDINATES: protein motif:HMM:NF025055.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pili methyl-accepting chemotaxis
                     transducer N-terminal domain-containing protein"
                     /protein_id="HNE05290.1"
                     /translation="MLQRLQVQLVILLTTFVLLVGVSASLTFWGLQTQQQDALVINLA
                     GRQRMLIQQMTRLALQLQGGDESALGELRESERMFGETLSALQNGGEAPYLEEGIASL
                     PATRDPQILAALQDMESNWLQYRSMLGTIAASPSESGLLQDDLGIQSKILVQKTDAVV
                     RLYEAASIAKVNRLRLIQFVFLISALALLAVGAWITRRSLLKPLLALGVAAKRLGKNQ
                     LDGAVQVQGPEEMRVLSQAF"
     gene            1316..2688
                     /locus_tag="PLT08_12270"
                     /pseudo
     CDS             1316..2688
                     /locus_tag="PLT08_12270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611865.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT          767 a          681 c          681 g          628 t
ORIGIN      
        1 gaacgcctgc gacaaaaccc gcatctcttc gggaccctga acctgcaccg ccccatcgag
       61 ctgattcttc ccgagtcttt ttgccgccac gcctaacgcc agaagcggct tcaacaggga
      121 ccgccgcgta atccacgcgc cgaccgccag cagcgccagc gcggaaatta aaaatacaaa
      181 ttgaatcagg cgcaggcggt tcacctttgc aatcgaagcg gcttcgtaca aacgcacgac
      241 cgcgtctgtt ttttgaacca gaatttttga ttggattcca aggtcgtctt gcagcaaacc
      301 cgactcggac ggcgaagcgg caatggttcc caacatggaa cggtattgaa gccagttcga
      361 ttccatatct tgcaacgcgg ctaaaatctg cgggtcgcgc gtcgctggca ggctggcgat
      421 accctcctcc aggtatggag cctctccccc gttttgcaaa gcggataaag tttcgccgaa
      481 catccgctcc gactcgcgca attcccccag cgcggactca tccccgccct gcaattgaag
      541 cgcaaggcgg gtcatctgct ggatcaacat ccgctgacgc cccgcaaggt tgattacaag
      601 cgcgtcctgt tgctgagtct gcaaacccca aaaggtcagg ctcgcagaaa caccgacaag
      661 caaaacgaat gtggtgagca ggatgaccag ttgaacttga agacgctgta acatggggtt
      721 ggcgaaaaat ccttgcgaga ggatttttca attatacctg ctacgcgtta agaatctggc
      781 gggaggcaag agcgctcttt ggagtctggt gtggtttccg ttcaatggaa agaaaaaagc
      841 cgccctttgc agatgtcatt gaaaagtgga aagcgccgca ttttaataca ttgaccaaat
      901 aagacttttt cacaatcgca atcggatctg tccgtcgggg tacatatctg gtatacgggt
      961 ataatttcgc cccacattga accacattta atacatacca caccctgccc ccatatatgg
     1021 agatagggtg ccaagaacag aataaaactc tccatatagc tgaatgatcg tatacggaaa
     1081 tggaatttct ctcataaacg atcatgccgc actgctctac ttgggagtgc gtacttaatg
     1141 aaaaaaatgc tttgataatc ctttctcagg attgtcaaag cgttatcctt ttataagctg
     1201 ctcattacag ccgagctatg tatggtgtca gaagaaatga gacacagggg cggaaaaagt
     1261 aaggtggaaa tcccggccaa aatctgatta aatcagaaaa cccaaggagt agaaaatggc
     1321 cgaagaaaaa gtctcatccg tggaagccac agtccgggag attcggcgga agacccgcaa
     1381 gaagtacaat gcggaagaaa aaatgcggat cgtgctggaa ggattacgtg gggaaaccag
     1441 catagccgaa ttatgtcgtc gcgaaggact gcatcccaat ctatattaca aatggagcaa
     1501 agagttcctt gaagcaggta aacaacgatt agtgggagac atccatcgcg aagcagacag
     1561 ccgtgaagtg gatgaaatgc gcagcgagat tgaacaactt aagcagttgg tagcagaact
     1621 gtcactcaag aatcgagtgc taaaaaaaag cttgctgggc aaggacactt catgggagga
     1681 ttaagcgtac ctgttgcgaa cattagtatg cgacacagcc agatagagaa acaggagatc
     1741 atccatctgg tggaacactc gcaattgtca gtcaggaaaa ccctggaaga attaaacgtt
     1801 cctgccagca ccttctatcg ctggtaccga aagtacgagg aagatggcgt ggatggcttg
     1861 atcgaccgca acccaagtcc gcgccaattc tggaatcgta ttcctgaggc ggtccgcaag
     1921 caggtggtgg atctggcatt ggaccatcca gaccactctt caaggcagat tgcctggctt
     1981 tttaccgacc aacagggcta tttcatctcg gaatcaagcg tgtttcggat tctgaagcgc
     2041 tttgatctgg tggagagtcc cgccttccga gtattgagcg cagcagacaa attcgtgcac
     2101 cctactaaac gggtgaacga gttgtggcag acagacttca cttatttcaa gatcttgaac
     2161 tggggctggt actacctatc tactgtgctg gatgattatt cccgctacat tctggcttgg
     2221 aaattgttcc ccaccatgtc agccaatgat gttcaggtta cgcttcagat cgccctcgac
     2281 aagactggct tggatcaggt cttggtggaa catcgtccgc ggctgttatc agataatggt
     2341 tcttgttatc tgtccaaaga cttaaaggat tttttggatc ggaagcacat ggaacacaca
     2401 cgtggcgcgc cttatcaccc tatgactcag ggcaagatcg aacgctatca tcgctccatg
     2461 aagaacattg tgaacttgca gaactacttc ctgccggagg aattggaacg ggaagtcgca
     2521 tcatttgtgg agtattacaa ccaccagaga tatcatgagt ctctggacaa tttaactccg
     2581 gcagatatgt tttatggcag agccaaggag gtactaaccc gacgacagga gatcaagcta
     2641 agaacgttac aggaacgacg cttgcagaac ctgcgagctg ctgtataatc ccaaccatcc
     2701 gggaaatcta caccttaact attcgctctt tgtgtctcat ttcttctgac accatac
//
LOCUS       DAOQJE010000490         2756 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_25588_length_2756_cov_7.228064, whole genome shotgun sequence.
ACCESSION   DAOQJE010000490 DAOQJE010000000
VERSION     DAOQJE010000490.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2756)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2756)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2756
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25588_length_2756_cov_7.228064"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..396
                     /locus_tag="PLT08_12275"
     CDS             <1..396
                     /locus_tag="PLT08_12275"
                     /inference="COORDINATES: protein motif:HMM:NF015317.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqhA family protein"
                     /protein_id="HNE05291.1"
                     /translation="LIASFAAFAWGTIKTVTTTLLVIETLGKDSSITIELIELIDIFL
                     IATTILIFSASLYELFIGKLDMPEWMLAHDLYELKAKLSSMIVLVMAVKYLQKLIEIK
                     DGTELLQRGIAVAIVSAALIAFGYFGKKD"
     gene            425..979
                     /locus_tag="PLT08_12280"
     CDS             425..979
                     /locus_tag="PLT08_12280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE05292.1"
                     /translation="MTGLPPFQQGGLDSLCGLYSIINAERIINHSTDDESQALFDGLI
                     RYLSKRGLLGKFLVGGILHPQMLLILNKVVGKKRISNVNIPWRGVPNPDLTTFWKSMQ
                     SFLDGTQGRAIILGIMGYHEHWTVIEKITNRSILLYDSALIKRLPRNFCTTVYATGQR
                     KHVLLPAQTYFLSNEVFDITQYDT"
     gene            1016..1378
                     /locus_tag="PLT08_12285"
     CDS             1016..1378
                     /locus_tag="PLT08_12285"
                     /inference="COORDINATES: protein motif:HMM:TIGR00026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family deazaflavin-dependent
                     oxidoreductase"
                     /protein_id="HNE05293.1"
                     /translation="MTLDLAPFEKEEYCYLTTKGRKTGTPHEIEIWFTIHENAVYLLS
                     GGMDKSDWVKNLLQDPNVTLRIAGQTFSAFASILEDKELEPMIRMAMAIKYDEWEGQD
                     PSEWARTALVVKFIPQSL"
     gene            1640..2455
                     /locus_tag="PLT08_12290"
     CDS             1640..2455
                     /locus_tag="PLT08_12290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE05294.1"
                     /translation="MNTIRKSFSVIFSFLCIGLLIACIPYTPPPPPPPPGVALTLTVQ
                     AIQLGFFTQTAQALQSQPIVIVVTATPDPNNLAPTTDPNAAPAATTSSAVTITVSQNT
                     NCRLGPSQDFDEIGSLGPGQTAEVVGKDTYDNYWVIKLPNNPAITCWLWGQYATVTGD
                     ISTVADSATPTAISNGAPNAPSLLDAQVKCTKLSGKDYQYKITLKWADNSSDENKFVI
                     NTSTGKTFSTPPNKTSFSFEEGLPSETNLSATITAANEKGESQPVGTGAFKCP"
     gene            2570..>2756
                     /locus_tag="PLT08_12295"
     CDS             2570..>2756
                     /locus_tag="PLT08_12295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE05295.1"
                     /translation="MNAARKSLILLVVLILSTLACGLENIQVVEAPNLEATITAQAAL
                     LQQGGQPAQDPAQQVIVV"
BASE COUNT          763 a          743 c          563 g          687 t
ORIGIN      
        1 ttgatcgctt ctttcgccgc ctttgcctgg ggcaccatca aaacagtgac tactaccctg
       61 ttagtaatcg aaaccctcgg caaagacagt tccatcacca tcgagttgat cgaactgatc
      121 gatattttcc tgatcgccac caccattctg attttttctg ccagcctgta cgaactcttc
      181 atcggcaaat tagacatgcc cgaatggatg ttggcacatg acctgtacga actcaaagcc
      241 aaactcagca gcatgatcgt gctggtaatg gcagtcaagt atttgcaaaa actgatcgaa
      301 atcaaagatg gaactgaact cctccaacgc ggcattgccg ttgccatagt ctctgcggcg
      361 ctgatcgcct tcggatattt cggcaaaaag gactgatggc accatgagaa ggtcaagccg
      421 cttaatgacg ggacttccgc cctttcaaca aggcggcttg gactcacttt gcggcttgta
      481 cagtattatc aacgccgaac gcatcattaa ccattccacc gatgatgaat cgcaagcctt
      541 gtttgacgga ctgatccgct acctttccaa gcgcgggtta cttggaaaat tcctcgtcgg
      601 cggaatcctc cacccacaga tgttgctgat tctgaacaag gtcgtaggga agaagcgtat
      661 ctccaacgta aatattccct ggcgtggcgt tcctaaccca gacctcacca ccttctggaa
      721 gtcgatgcaa tcctttttag atggcaccca aggtcgtgcc attatccttg gcatcatggg
      781 gtatcacgag cattggaccg tcatcgaaaa aattaccaac cgttctattc tgctatacga
      841 ctcggcactc atcaaacgct taccacgcaa cttctgcacc accgtctacg ccacagggca
      901 acgcaagcac gtactattac cggcacaaac ctattttctc tccaacgaag tttttgatat
      961 tacacaatac gatacctaga cttagcccac ctactgaatg attttggaga catatatgac
     1021 cctagacctt gctccatttg aaaaagaaga atactgttac ctcaccacaa agggacggaa
     1081 aaccggaacg cctcatgaga tcgaaatctg gttcaccatc catgagaacg cggtctacct
     1141 tctctctggc ggcatggaca aatcggattg ggtcaaaaac ttgctgcaag accccaacgt
     1201 cactcttcgc attgcgggtc aaaccttctc tgccttcgcc agtatattgg aagacaaaga
     1261 actagaaccc atgatccgca tggcaatggc gatcaagtat gatgagtggg aagggcaaga
     1321 ccctagtgaa tgggcacgga cagcattggt agtgaagttc attccccaaa gtttataaag
     1381 tcatttgtga taaataacat attacgagta aattacagta cttaagaaca ccttacgtaa
     1441 atgaaaagtc cagtaaaatg ggggaaatta tctaattggg gacatttagt tttgccaatt
     1501 ttatttcatg ctctttgcca tgtgccaagc tccgcacagg taaagccttt cataacgccg
     1561 gagtgaaagg gatgactatt tggcaaatat acaactacct ccattttagg gaattcccat
     1621 ttcgtacagg agctgctcca tgaacactat tcgtaagtca ttttctgtaa ttttctcctt
     1681 tttatgtatt ggtttactta ttgcttgtat tccatatacc cctccccctc ccccgcctcc
     1741 gcctggggtt gcgttgacgc tcaccgtgca agccattcaa ctgggcttct tcactcaaac
     1801 tgcccaggca ttacaatctc aacccattgt tattgttgtc acggctacgc ctgacccgaa
     1861 caatcttgct ccaacaacag accctaatgc agccccagcg gctactacat ccagcgcggt
     1921 gacgatcacc gtcagccaga acaccaactg tcgtctcggt ccgagccagg actttgacga
     1981 gattggctcc ttgggacctg gtcagaccgc agaagtagtc ggtaaggata catacgacaa
     2041 ctactgggtc atcaaacttc ccaacaaccc tgcgatcaca tgctggttat ggggacaata
     2101 tgccacagtg acgggtgata tctcaaccgt tgcagatagc gccacgccta ctgctatttc
     2161 caatggtgct cccaatgccc catctctttt agacgcccaa gtgaaatgca ccaaactgag
     2221 cggtaaagat tatcaatata agatcaccct caaatgggcc gataactctt ccgacgaaaa
     2281 taaattcgta atcaatacgt ccaccgggaa aaccttctca acaccaccca ataaaacctc
     2341 tttcagtttt gaagaaggtc ttccttccga gacaaacctt tctgccacaa taactgccgc
     2401 caatgaaaag ggcgaatctc agccggtagg aactggcgcc ttcaaatgcc cataaaaggg
     2461 catccctact atctagccta agtaaaagaa aattcaccac agagaaggtt ttattttctc
     2521 tgtggtgaaa atctattcag caattatctc tgaacgagga gaattaagaa tgaacgccgc
     2581 ccgaaaatct cttatcttgc tagttgtcct catcctatcc acgctggctt gcggactgga
     2641 aaatattcaa gtcgttgaag cgccaaacct ggaggcgacc atcaccgcac aagccgcctt
     2701 gttgcaacaa ggcgggcaac ctgctcaaga tcctgctcaa caagtcatcg tggttg
//
LOCUS       DAOQJE010000491         2753 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_25640_length_2753_cov_4.963677, whole genome shotgun sequence.
ACCESSION   DAOQJE010000491 DAOQJE010000000
VERSION     DAOQJE010000491.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2753)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2753)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2753
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25640_length_2753_cov_4.963677"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..681)
                     /locus_tag="PLT08_12300"
     CDS             complement(<1..681)
                     /locus_tag="PLT08_12300"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNE05296.1"
                     /translation="MALITTSNLTKSYGATDIFTGLTVSIEKGERLGIVGPNGVGKTS
                     LLRILAGEDESSSGSVTRTRGVRTGYLSQEADFKMEGTLWDACHSVFDDLIRQQKELH
                     RLEELMATDPDVIEQYGKMQEDFERRGGYTYEIRMKQVLSGLGFDASDYSLSLDHLSG
                     GQRTRGFLARLLLSNPDVLLLDEPTNHLDIRAVEWLEGYLNQWDGAAIIVSHDRYFLD
                     KVSTEILEM"
     gene            854..1366
                     /locus_tag="PLT08_12305"
     CDS             854..1366
                     /locus_tag="PLT08_12305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE05297.1"
                     /translation="MYNEPQNSAISEDRSSSRKLIITIVAVLLFGCCCLVTGVGGYYG
                     YQAYVAAKEVVDQAKEIYEIPVNPDDPNSPNVPIPFTPFSEAPKGGLGDERAREFVWT
                     QIPVYYQEANCPSPKADTTEITVVTQPNAEGSWQEEWKVDCGNGTFHTFVVDFVTQDG
                     FSSGIVQPPQ"
     gene            complement(1444..2286)
                     /gene="proC"
                     /locus_tag="PLT08_12310"
     CDS             complement(1444..2286)
                     /gene="proC"
                     /locus_tag="PLT08_12310"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004735 - pyrroline-5-carboxylate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HNE05298.1"
                     /translation="MLKKKIAFIGPGVMAEAMIAGLLNKKLADAKNIIASGPRDARGE
                     ELTKKYGIKFTTDNSAAIHEADVVVLSVKPQRLTEVMKGLKGVRPDALVLSIIAGATI
                     KKIGAGLKHKAIVRSMPNTPGQIGEGITVWAASKEVTEEQQEMTRAILGALGEEVFVE
                     DESYLDMATALSGSGPAYVFLFTEALIDAGVHMGFPRRIAEQLVLQTIKGSASYYQGA
                     ARHPATLRNQVTSPGGTSAEALYYLEKAGFRTAISRAVWAAYQRSLELGKEKPGHVEV
                     DEEK"
     gene            complement(2365..>2753)
                     /locus_tag="PLT08_12315"
     CDS             complement(2365..>2753)
                     /locus_tag="PLT08_12315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE05299.1"
                     /translation="AWFNIGTSHVDLLEYADAASAYDYAFSLYADLNVDDTARPYRMM
                     WYQTGPYKAYFYSNRFTDVINLADTTLNDTIAEPVLEESLYWRGRAYVMSGDTNRAVK
                     DFMAALKVHPKWEPAVQALQDLGLQP"
BASE COUNT          665 a          683 c          702 g          703 t
ORIGIN      
        1 catttccaaa atttcggtgc tgaccttatc caaaaaataa cggtcatgcg aaacgatgat
       61 tgccgcgcca tcccattgat tcaaatagcc ttccagccat tccaccgcgc ggatgtcgag
      121 gtggttggtg ggttcatcga gcaggagcac atcggggttc gagagcaaga ggcgggcgag
      181 aaagcctcga gtgcgctgcc cgcctgagag gtgatccaaa gacaggctgt agtctgaggc
      241 gtcgaatccc aaaccagaaa gcacctgctt catgcgaatc tcgtaagtgt agccgccgcg
      301 tcgttcaaaa tcctcttgca tcttgccgta ttgttcgatg acatctgggt cggttgccat
      361 caattcttcg aggcggtgca attctttttg ctggcggatc aaatcatcga agacggaatg
      421 acacgcgtcc caaagcgtgc cttccatttt gaaatcggct tcttgcgaca aatacccggt
      481 tcggactccc cgtgtgcggg tcactgaacc cgagctggat tcatcctccc cggcaaggat
      541 gcgcaacagg ctggtcttgc ccacgccgtt cggaccgaca atgccgagcc gttctccctt
      601 ttcaatggag acggtcagcc cggtgaagat gtcggttgcg ccgtaggatt tggtgaggtt
      661 agaggtggta atgagtgcca tgagttttaa tgcatagtaa aaatgcgggt ggattttaac
      721 ccatctgcgt ttggtttgtt gaaaatgcgt agatttggat tgcaagaaaa attgataaaa
      781 ccaaaggggg attaaggttt tggctctttt ggagataata cacattaatt attgtttcag
      841 gagatgttta taaatgtata acgagcccca aaattctgcc atcagtgaag acagaagttc
      901 aagccgaaag ctaattatta ctattgtggc agttcttctt tttggttgtt gctgtcttgt
      961 cacgggggtg ggcggctact atggctacca agcctatgtg gcagcaaaag aagtggtaga
     1021 ccaagccaag gaaatctatg agattccggt caaccccgat gaccccaaca gccccaatgt
     1081 tccaattccg ttcacccctt ttagcgaagc accaaaaggc gggcttggag atgaacgagc
     1141 gcgtgaattt gtatggacac agattcctgt ttattatcaa gaagcaaatt gcccctctcc
     1201 caaagcggat acgactgaaa tcacagtggt cacccagcct aatgccgaag gctcatggca
     1261 ggaagaatgg aaagtggatt gcggcaatgg caccttccat acttttgttg tagattttgt
     1321 gactcaggat ggattttctt caggaattgt tcagccacct cagtaattac tatatgtgaa
     1381 ataaaaacgc ggatagatgt tcatctatcc gcgttttgtt tgaagtaacc taattacgaa
     1441 attctacttc tcttcatcca cctcaacatg acccggcttt tccttgccca attccagtga
     1501 gcgttgatac gccgcccaaa ccgcgcgcga aatcgccgtg cggaagcctg ccttctctaa
     1561 ataatacaat gcctcagcag aagtcccacc gggcgaagtg acttgattgc gcaaggtggc
     1621 aggatgccgc gccgcgcctt gataatacga agctgagccc ttgatggtct gcaagacaag
     1681 ttgctctgca atgcggcgtg ggaagcccat gtgaacgccc gcgtcgatga gagcttcggt
     1741 gaagagaaag acatacgcgg gtcccgaacc ggagagcgcg gttgccatgt cgagatagct
     1801 ttcatcttcc acaaacactt cctcgcccaa cgcgccgagg atggcacgcg tcatttcctg
     1861 ttgttcttcg gtcacttctt tcgatgccgc ccacacggta atgccttcac cgatctgtcc
     1921 cggtgtattg ggcatcgagc ggacgatggc tttgtgcttc agccccgccc caatcttttt
     1981 gatggtcgcg cctgcaataa tggataagac caacgcatct ggtcggactc ctttgagccc
     2041 tttcatcact tcggtcaggc gctgcggttt gacggagagc accaccacgt ccgcttcgtg
     2101 aatggcggca gaattatcgg tggtgaattt gatgccatat ttcttggtca gttcttcgcc
     2161 acgtgcatct cgcggaccag aagcgataat atttttggcg tctgccaatt ttttatttag
     2221 caatcccgcg atcatggctt ctgccatcac accgggaccg ataaatgcaa ttttcttctt
     2281 aagcatggtt ctctcctatt acgttacgtg aaagatatta tacaaagaaa gaagggatgc
     2341 agaagcttct gcatcccttc ggttttacgg ttgcaagccc aaatcttgga gcgcttgcac
     2401 ggcgggctcc catttggggt gaactttgag cgccgccatg aaatctttca cagcgcgatt
     2461 ggtatctcca gacatcacat acgcccgtcc gcgccagtat agggactctt ccaacacggg
     2521 ttcggcgatg gtgtcgttca gggttgtgtc tgccaagttg atcacatcag taaagcggtt
     2581 ggaatagaaa taggctttat acggaccggt ctgataccac atcatgcgat agggacgggc
     2641 ggtatcgtca acattcaaat ctgcatataa gctaaatgca taatcatagg cgctggcggc
     2701 atcggcgtat tcgagcaagt ctacatggct ggtgccgatg ttgaaccagg caa
//
LOCUS       DAOQJE010000492         2749 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_25718_length_2749_cov_3.555679, whole genome shotgun sequence.
ACCESSION   DAOQJE010000492 DAOQJE010000000
VERSION     DAOQJE010000492.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2749)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2749)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2749
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25718_length_2749_cov_3.555679"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..203
                     /locus_tag="PLT08_12320"
     CDS             <1..203
                     /locus_tag="PLT08_12320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE05300.1"
                     /translation="GLKQVLASSGPLVCEVILNPDQPFEPKISSRQLPDGKIVSAPME
                     DMFPFLPREEMLSNLLIPPLEE"
     gene            205..876
                     /locus_tag="PLT08_12325"
     CDS             205..876
                     /locus_tag="PLT08_12325"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013530.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HNE05301.1"
                     /translation="MTIRKVLITGGARGIGKAIAEKFTASGFEVHAPTRSEMDLSDEN
                     SVQAFIERNRQITFDVLVNNAGENIVGGLQELPLADWKRMQAINITAPFLLTQFVSGQ
                     MRLQNWGRIVNISSAYSTITRVGRGGYSATKAAINSLTRSAALEFAENNILVNAVAPG
                     FLETDLTRKNNSPQQIENLRSQVPMKRLGEPQEVAELVYFLGSSLNTYITGQVIFIDG
                     GFMAQ"
     gene            884..1963
                     /locus_tag="PLT08_12330"
     CDS             884..1963
                     /locus_tag="PLT08_12330"
                     /inference="COORDINATES: protein motif:HMM:NF013887.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE05302.1"
                     /translation="MRSLNIKSGQGDYRVDFHDSIQNLVNAVLQTGVSAIVIDQNVAA
                     LYRNILHPLTDKIPVLEIPATEEEKTLAGIEKTLAFFQQNNLTRNSIVLAIGGGIIQD
                     IVTFSSHVYYRGIRWMYIPTTLLSMCDSCIGAKCGINFGAHKNQLGIFQSPAHVFISS
                     QFTQTLSERDVRSGYGEMLKLIITGSEDLFQQYRSAFEVGFPSLGDANHFIYESLNVK
                     KRVIEIDEYELDLRRILNYGHTFGHALESLTKYEIPHGSGVAWGLDLANFLGWRLGVT
                     READFLRIHEFVMKHFRFRLSAPVDSRQLVDMTRRDKKVTQGKANLILLREPGDLAII
                     PVEYDEKLYGLVDEFLKEYNVIYWD"
     gene            1944..>2749
                     /locus_tag="PLT08_12335"
     CDS             1944..>2749
                     /locus_tag="PLT08_12335"
                     /inference="COORDINATES: protein motif:HMM:NF012589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY family carbohydrate kinase"
                     /protein_id="HNE05303.1"
                     /translation="MSFIGIDLGGTFIKGAILDADLGQLRHIRRRPFPEFESIESLER
                     VVSPEKVLRSLHDLLDELFLLSPDCEGIFLCGQMHALVMCDALGNPHSEVITWQDQRA
                     GQPFEGSSQSTLDALKAILKDWDLRRLGNEFRVGLPLTQLFHLKRSDQLPKKLYPASL
                     MDFVVSNLCKTPPVTDSTNAEAHGLFDLLTGSWDEQLIRYLGLDGLTWQEIKNHGTVA
                     GTFIHHGRAIPCYLPVGDQQAALLGSFLQPRMLSLNIATGSQVGLLSSEC"
BASE COUNT          693 a          589 c          684 g          783 t
ORIGIN      
        1 gtgggctcaa acaagtgctc gcctcaagcg gacctttggt ttgtgaggtg attctcaatc
       61 ccgatcagcc cttcgagccc aagatcagct cccgccagct tccggatgga aaaattgttt
      121 ctgcaccgat ggaagatatg ttcccattcc tgccgcgtga agagatgttg agcaatctgc
      181 tcattccccc gttggaagaa taaaatgacg attagaaaag ttttgatcac cggtggggcg
      241 cggggaattg gcaaagccat tgctgaaaaa tttaccgcgt ctggatttga agtccatgcg
      301 cctacacgct ccgagatgga tttgtcggac gagaactccg tgcaggcttt tattgagaga
      361 aatcgccaga tcacatttga tgtgcttgta aataatgccg gggaaaatat tgtcggtgga
      421 cttcaggaac tgcccttagc agattggaaa cgtatgcaag ctataaatat tacggctcca
      481 tttcttctca cgcagtttgt ttctgggcaa atgaggctcc aaaattgggg gcgtatcgtt
      541 aatataagct ctgcgtatag tacgatcaca cgggtaggtc gcggcggata cagtgcgaca
      601 aaggccgcga tcaactctct tacccggtca gccgcgttgg aatttgccga gaataatatt
      661 cttgttaatg cagtggcgcc tggttttctc gaaacagacc taacgcgaaa gaataactca
      721 ccgcaacaga tcgagaacct gcgctctcaa gtaccaatga aacgattggg ggagccgcaa
      781 gaggttgctg agttggtgta ttttctaggc tccagcttga atacatatat tacggggcag
      841 gtcatcttta ttgatggcgg cttcatggca caataggtga cgtatgagat cattgaatat
      901 taagtctggt cagggtgatt atcgggtcga ttttcatgac tcaatacaaa atttggtgaa
      961 tgctgtactc caaacgggtg tttccgccat cgtgatagac cagaatgttg ctgcgcttta
     1021 ccgcaatatt ttgcatcctt taaccgacaa gattcctgtt ttggaaatcc ccgctaccga
     1081 agaagaaaag actctggctg gaatagaaaa gacccttgcc ttttttcaac agaataatct
     1141 cacccgtaat tccattgtgc ttgccattgg agggggaatc attcaggata ttgttacatt
     1201 ttcttctcac gtctattacc gtggcattcg ctggatgtac attccaacaa cactgctttc
     1261 tatgtgtgat agctgcatcg gcgcaaaatg cggtatcaat tttggcgcgc ataaaaatca
     1321 gttgggcatt tttcagtctc cggctcacgt attcatcagc tcgcagttta ctcaaaccct
     1381 ttcggaacga gatgtccgtt cggggtatgg tgagatgttg aagctgatca tcaccggctc
     1441 tgaggaccta tttcagcaat accggtcagc tttcgaggtg ggatttcctt cgttaggtga
     1501 tgccaatcat tttatttatg aaagcctgaa tgtgaaaaag agagtcattg agatcgatga
     1561 gtatgaactc gatctgcgcc ggatccttaa ttatggtcat acctttgggc atgccttaga
     1621 gtcactgaca aagtatgaaa ttccgcatgg ctcaggagtt gcttgggggc tggatttggc
     1681 aaactttttg ggctggcggc ttggggtcac ccgtgaagcg gattttcttc gaatccacga
     1741 atttgttatg aaacacttcc ggtttcggtt gtccgcgcct gtggatagcc gtcagctagt
     1801 ggatatgacc cgccgcgata aaaaggtaac tcaaggcaag gcaaatctta tcttgttgcg
     1861 tgagccgggt gatcttgcca ttattcctgt ggagtatgat gaaaaactct acggattggt
     1921 cgacgagttt ttgaaggaat acaatgtcat ttattgggat tgaccttggt ggcactttca
     1981 tcaaaggcgc tattttagat gcggatctcg gacaactgag gcatattcgc cgtcgacctt
     2041 tcccagaatt cgaatcaata gaatcgcttg agcgggttgt ttcgccagaa aaagtattaa
     2101 ggtcgcttca tgaccttctg gacgaactat ttctgctctc accagattgt gaaggtattt
     2161 ttctgtgtgg gcaaatgcat gcgttggtta tgtgcgatgc acttggaaat cctcattctg
     2221 aggtcattac gtggcaagac caacgcgccg ggcaaccatt tgaaggatcg agccagagca
     2281 ccttagatgc actgaaagcg attttgaaag attgggattt gcgaagactt gggaatgaat
     2341 ttcgtgtggg gcttccatta acccagttat ttcatttaaa acgctctgat caactaccta
     2401 aaaaattgta ccccgccagt ttgatggatt ttgttgtttc caacttgtgc aaaaccccac
     2461 ccgtaacaga ttctacgaat gcagaggcgc atggattatt tgatcttttg actggcagtt
     2521 gggatgaaca gttgattcgc taccttgggc tagatggact tacatggcag gaaataaaga
     2581 atcatggcac tgttgctggc acattcatac atcatggacg ggcaatcccc tgttatttgc
     2641 cagtaggcga ccaacaagca gctttattag gaagtttcct tcaacctcgg atgctttctt
     2701 tgaatattgc cactggttcg caggtgggat tgctttccag tgagtgtga
//
LOCUS       DAOQJE010000493         2747 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_25743_length_2747_cov_4.336181, whole genome shotgun sequence.
ACCESSION   DAOQJE010000493 DAOQJE010000000
VERSION     DAOQJE010000493.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2747)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2747)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2747
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25743_length_2747_cov_4.336181"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(155..1054)
                     /locus_tag="PLT08_12340"
     CDS             complement(155..1054)
                     /locus_tag="PLT08_12340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE05304.1"
                     /translation="MEDHESPNSDSQTYLDNLDDSRKGRYVNIGIFIVLIFLGVFGMY
                     IKLRSPDITIAQLTATETQVTSTAIRLTASPTTTATLAKEVVQITSTSTPSTITDFAQ
                     VNYEAQISCEEISKVNLRKSPGYANKNDNKDVIVEIPCGESLNLQLETKFADELTWWK
                     VNWKSYEGWVSDHTGSGKTILTFTEPSSFSQSRPEEFVFWYFNAMWQQRNYEDLWNNF
                     LTTSFQNHSSNGNFNEYVKWWKTVSRIDINGVEIVQKKEISAWAKINVNFYLNDGRVL
                     NSREYDYSLVFDTDKRIWMFDYP"
     gene            complement(1103..1825)
                     /locus_tag="PLT08_12345"
     CDS             complement(1103..1825)
                     /locus_tag="PLT08_12345"
                     /inference="COORDINATES: protein motif:HMM:NF024183.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HNE05305.1"
                     /translation="MKELNLRKKKFYSATMRSFILLVVLFILLAVSGDIKMLQNQKII
                     LNKGELNQTIITGVIIAKALLTLFIVSILLNFAYVAETQLPFIMSKIPQSGLVVSSAV
                     HIGVIFIAYFNLLEVATERNTVNQVFNAVFLLLLCIPLFRGGKALYDGIDRFANQAAN
                     VFDESKPSINTALQSIICKNCNTENDISAKHCIECGNNLQEPKTTQQFISCPQCGEKN
                     QGNAKHCSECGTNLTKTAAKSM"
     gene            complement(1847..>2747)
                     /locus_tag="PLT08_12350"
     CDS             complement(1847..>2747)
                     /locus_tag="PLT08_12350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE05306.1"
                     /translation="PEILKKYDDDIPNGSIIESNKDETYLLHIKSETQTWKSYIEQIA
                     KLSLALDTIMDTQQEAETRISQNNKPFDNVTDDIQKIWGAITDKADTITNDKASEVQK
                     RLIKWFFSSISGVKGKTKEFTKQAKELSPKLSDLENLLELEIAQFQAYASKIKKEEIA
                     ILGARVSSTIIIPQLKNRIDNCKKEIHDYNNYLENLSVELERQNISRQIYEILANEYK
                     EALEATSNLLNKSLAEAEIWKREGVVLLESVIQSANKELEIIRARKLSGQIVDIDDVK
                     KKSKSLNDEIRRLEKAKELIGLL"
BASE COUNT          745 a          504 c          501 g          997 t
ORIGIN      
        1 gggctaacgg tttgcgttac ctgcgtgtgg gcgggcgtgg attctgcttg ggagcaggaa
       61 aaactcgaag cgagaaaaat gcttgaaaat gccgcagaat cccacacgtc aggtgcacgc
      121 tttgttggct tgcggttgtt aacagtgacc tttgctatgg atagtcaaac atccaaatcc
      181 ttttgtcggt atcgaacact aaactgtaat catattcacg actatttaat acacgcccat
      241 catttagata aaaattgaca ttgatttttg cccaagcact aatctctttt ttttgtacaa
      301 tctcgacacc attaatgtca attcggctta ccgttttcca ccacttgaca tattcattga
      361 agttgccatt gcttgaatgg ttttgaaaac ttgtggtgag aaaattgttc cataaatctt
      421 cgtaatttct ttgttgccac attgcattga aataccaaaa tacaaattct tcaggacgag
      481 attgggagaa tgatgatggt tctgtaaaag tcaatattgt tttaccactg cctgtatggt
      541 cagataccca gccttcataa cttttccaat tgactttcca ccaagttaat tcatctgcaa
      601 attttgtttc caattgaaga ttaagggact caccacatgg aatttcaaca attacatctt
      661 tgttgtcatt tttgttggcg taccctggtg atttacgcaa gttaacttta ctaatttcct
      721 cacaggaaat ttgagcctca tagttaactt gggcaaaatc tgtaatagta gatggcgttg
      781 atgttgaggt aatttgaacc acttcctttg ctaaggttgc agtagttgtg ggtgatgcag
      841 taagacgaat agcagtagat gtaacttgcg tttctgtggc tgttaattgt gctatcgtta
      901 tatcgggact tcttagctta atgtacatgc caaaaactcc caagaaaatt agcacaataa
      961 aaataccgat atttacataa cgtcccttcc ttgaatcatc aagattatcc aagtacgttt
     1021 gggaatctga attgggactt tcatggtctt ccatgttatg gctcatttgg ctttttgaag
     1081 cagaagtttt tttgcagaaa ctttacattg acttggcggc tgtcttcgtt agatttgtgc
     1141 cacactcgct acaatgtttt gcatttcctt gattcttttc gccgcactgg gggcatgata
     1201 tgaattgctg agtggtcttt ggctcttgaa ggttgtttcc gcattcaata caatgctttg
     1261 cagagatgtc gttttcagta ttgcaattct tgcaaattat agattgtaaa gcagtgttaa
     1321 ttgaaggctt agattcgtca aatacgttcg cagcttgatt tgcaaacctg tctattccat
     1381 cataaagagc tttgccaccc ctaaaaagtg gaatacagag taaaagcaag aaaacggcat
     1441 taaatacttg atttacggta ttcctctctg ttgcaacttc taacaaattg aaataggcaa
     1501 taaagataac gccaatgtga acagcagagg agactaccaa tccactttga ggaatttttg
     1561 acataataaa aggaagttgg gtctcggcga cataggcaaa atttagaagt atgctgacaa
     1621 tgaataaagt taggagggct tttgcaataa tgacacccgt tattatggtt tggttaagtt
     1681 cacctttatt caatatgatt ttttgatttt gcaacatttt aatatctcct gataccgcca
     1741 ataggataaa tagcactact agcagaataa aagacctcat tgttgcactg tagaacttct
     1801 tctttcgtaa atttagctct ttcatggaaa acctcctttg aattttttat aacaaaccaa
     1861 tcaattcttt tgctttttct aatctgcgga tttcgtcatt gagtgacttg cttttctttt
     1921 tcacatcatc tatatccacg atttgaccag atagttttct cgctcgaatt atttctagtt
     1981 ctttatttgc tgactggatt acactttcta ataaaactac gccttccctt ttccatattt
     2041 ccgcctctgc taaagatttg tttaagagat tgcttgttgc ttcaagagct tctttgtatt
     2101 catttgcaag gatttcgtaa atttgtctcg aaatattttg tctttcgagt tccacactta
     2161 aattttccaa gtaattattg taatcatgta tctctttttt gcaattgtct attctgtttt
     2221 taagttgggg aataataatt gttgaggaga ctcgcgcgcc aagtattgca atttcttctt
     2281 tttttatttt acttgcatac gcttgaaatt gtgcaatttc tagctctaaa agattttcta
     2341 aatcagacaa ctttggtgat aattcttttg cttgttttgt gaattctttc gttttacctt
     2401 tgacaccaga gatagatgag aaaaaccatt tgattaaccg cttttggact tcacttgctt
     2461 tatcatttgt gattgtatct gccttatccg ttatcgcacc ccaaattttt tgtatatcgt
     2521 cagtaacgtt atcaaaaggt ttgttatttt ggctgattcg agtttcagct tcttgctgag
     2581 tgtccataat ggtatcaaga gctaaactta actttgcgat ttgttcaata taacttttcc
     2641 atgtttgagt ttctgatttt atgtgcagaa ggtaggtttc gtctttatta gattctatga
     2701 tgcttccatt gggaatatcg tcgtcatatt ttttcaaaat ctcaggc
//
LOCUS       DAOQJE010000494         2746 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_25755_length_2746_cov_5.678558, whole genome shotgun sequence.
ACCESSION   DAOQJE010000494 DAOQJE010000000
VERSION     DAOQJE010000494.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2746)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2746)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2746
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25755_length_2746_cov_5.678558"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..349
                     /locus_tag="PLT08_12355"
     CDS             <1..349
                     /locus_tag="PLT08_12355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE05307.1"
                     /translation="TERQITNDGKTTNAVWSPEGDRIAYQSERSGNLDIYSYDFRDNK
                     EYRVTDFDGKDSGPTWDCGGTNLAFTSVRDGDLNIFQVFWQGGSAGNMTIDPASDKWS
                     QWRPSNDISSVGY"
     gene            507..1064
                     /gene="efp"
                     /locus_tag="PLT08_12360"
     CDS             507..1064
                     /gene="efp"
                     /locus_tag="PLT08_12360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HNE05308.1"
                     /translation="MIDVNELRKGVTFELDGNLYKVLDYSHNKTGRGNATIRVKARNL
                     LTGANIERTFNSGQSVQDVSLDFANVSYLYSDGDLYYFMDKVTFEQPGIKAESLGESA
                     QYLKEGMEVKLTFYKGEAIDVEMPTSVDLKVVEAEMAIRGDTATGVTKKVTTETGLTV
                     QCPNFVNVGDTIRVDTRTGEYVTRV"
     gene            1070..1402
                     /locus_tag="PLT08_12365"
     CDS             1070..1402
                     /locus_tag="PLT08_12365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE05309.1"
                     /translation="MKTPAGHECPHFYGDYFRGRNVEECRLLKSQNQAWSPDLCKTCP
                     VPAIARANSCQFMKLNVVVARPLTAIFQRRVQVQAYCEKSNQNVSEPQVGCGECHALP
                     FKFEIKED"
     gene            1433..2626
                     /locus_tag="PLT08_12370"
     CDS             1433..2626
                     /locus_tag="PLT08_12370"
                     /inference="COORDINATES: protein motif:HMM:NF024811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HNE05310.1"
                     /translation="MKLTLLLDLDETLLNTNQDVFFPAYIQALSTNFASQLDPKLVMR
                     ALMTSIEKMNQNDDASRTLKDVFSDEFCPQVGMTRQELDAHIDVFYSQVFPTLRGITS
                     QIAETQPFIEWAMKQGYRLVVATDPLLPRPATRQRVQWAGLDPDQFELISTFEDFHFL
                     KNFSAYYAEVLGQLGWQEGPILMVGNDAERDILSAKRLGLATFFVETESGSTSGPEAG
                     PRGTLSDLRRYLESTDLASLTPSFKTKEAIMAIQASVPAVLDTFFRGLSAETWKHKPA
                     SSEWTLTELVCHLRDTEREIHHMQLKLFNGQDEPFIPRPDTGIWANQRDYLHEDGPSA
                     LREFAKARSMTLSMLQNTPDVEWDRKARHAIFGPTNFREVAGFIADHDRLHVQQALSL
                     LKKLQ"
BASE COUNT          739 a          633 c          649 g          725 t
ORIGIN      
        1 aacggagaga caaattacca acgacggcaa gacgaccaat gctgtttggt ctccagaagg
       61 tgaccggatt gcctaccaat ctgaacgcag cggaaacttg gatatctata gctatgactt
      121 tagagataac aaggagtatc gtgtgacaga ctttgatggc aaagactcgg gtccgacatg
      181 ggactgtggt ggcacaaacc tggccttcac atcggttcgt gacggagact tgaacatctt
      241 ccaggtcttc tggcagggtg gttccgctgg aaatatgacc atcgaccccg cttctgataa
      301 gtggtcacag tggcgaccta gcaatgacat ctcaagtgtg ggctactaat aagatttgat
      361 tgcaaagagc ctctcctgtt ttttgaggag aggctctttt ttgtgcctca ttctttccta
      421 atgatcccat cctagccatg attcgctact cgttggtata atccgcccgc ttatacaaaa
      481 accaaataac caaggaaaat gcaagaatga tcgatgtaaa tgaacttcgc aaaggtgtca
      541 cttttgaatt ggacggcaac ctctataagg tgttggacta ttcacacaac aaaaccgggc
      601 gcggcaatgc gaccatccgt gttaaggcgc gtaatctcct taccggtgcc aacattgaac
      661 gcaccttcaa ctcgggtcaa tccgtgcagg atgtaagcct cgattttgct aatgtttctt
      721 acctctacag tgacggcgac ctttactatt ttatggataa ggtcactttc gaacagcctg
      781 gcattaaagc cgagtcactt ggtgaaagcg cccagtacct caaagaaggc atggaagtta
      841 agttgacctt ctacaaaggt gaagctattg atgtggaaat gcctacttct gtggacctca
      901 aagtcgtgga agctgaaatg gcgatccgcg gcgatactgc caccggcgta accaagaagg
      961 tcaccactga aaccgggctc actgtgcaat gcccgaactt cgtcaacgtt ggcgacacca
     1021 ttcgtgtaga cactcgtacg ggcgagtacg ttacccgcgt ctaataggaa tgaaaacacc
     1081 tgctgggcac gagtgcccgc atttttatgg cgactacttc cgcggcagaa acgtggaaga
     1141 atgtcgctta cttaaatcgc aaaaccaagc ctggtctcca gacctttgta agacttgccc
     1201 tgtgcccgct attgcacgcg cgaattcatg tcaattcatg aaattgaatg tggtggtggc
     1261 tcgtccacta acagctatat ttcagcgtag agtgcaagtg caagcctatt gcgaaaaatc
     1321 aaatcagaat gtttctgaac ctcaggtggg gtgtggcgaa tgccatgccc tgccttttaa
     1381 attcgaaata aaagaagatt aacccaaaga ctccgtagga gtctttttca tcatgaagct
     1441 cacattactg ctagaccttg acgaaaccct gctgaatacc aatcaggatg tctttttccc
     1501 tgcgtacatt caagcattgt cgaccaattt tgcatcgcag ttagatccaa aactggtcat
     1561 gcgtgcgtta atgacgagca tcgaaaagat gaatcaaaat gacgatgctt cccgtacatt
     1621 gaaagatgtg ttcagtgatg aattctgtcc gcaagtgggg atgacgcgcc aagagctaga
     1681 tgcacacatt gatgtgtttt atagtcaagt gttcccaact ttgcgcggta tcacttcaca
     1741 aattgcagag acgcagccat ttattgaatg ggcaatgaag caaggatatc gtttggttgt
     1801 ggcgactgac ccgttattgc cgcgcccagc gactcgtcaa cgtgtgcaat gggctgggct
     1861 agaccctgac caatttgaat tgatttctac ttttgaagat tttcactttc tgaaaaactt
     1921 ttctgcctac tacgctgagg tgctcgggca attaggctgg caagaggggc caattctcat
     1981 ggtggggaat gatgcagagc gggacattct ttcagcgaag aggcttgggc tggctacatt
     2041 tttcgtggaa acagaatccg gctcaacgtc tggacctgag gcgggaccgc gcggcacatt
     2101 atctgaccta cgccgttacc ttgagtctac tgatctcgca tccttaactc catcctttaa
     2161 aacaaaagaa gctatcatgg ctattcaagc atctgtacct gctgtgttag ataccttctt
     2221 ccgcggatta agtgcggaga catggaaaca taaacctgct tccagcgaat ggacgttgac
     2281 agaattggtc tgccatttgc gtgatacaga gcgcgagatt catcacatgc aattgaagct
     2341 tttcaacgga caagatgagc cgttcattcc acgcccagat acagggattt gggcaaacca
     2401 aagagattat ttacatgaag atggaccttc tgcattgcgt gaatttgcta aggcacggtc
     2461 tatgacatta agtatgttac aaaatacgcc agatgtagag tgggatcgca aagcgcgcca
     2521 tgcaattttt ggaccaacca acttccgtga agtggctggt tttattgcag atcacgatag
     2581 attacatgtt caacaagctc tttctttgct gaaaaaactt caataacgat tgggaatgcc
     2641 tcctaatatt gtctgatgac ctggtttttt aagcctaggt gtcatattcc cgcgtgtcca
     2701 attcccgaat cttgtgttaa ataggacaag tttgcttgtt ctattt
//
LOCUS       DAOQJE010000495         2742 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_25808_length_2742_cov_4.880536, whole genome shotgun sequence.
ACCESSION   DAOQJE010000495 DAOQJE010000000
VERSION     DAOQJE010000495.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2742)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2742)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2742
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25808_length_2742_cov_4.880536"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..226)
                     /locus_tag="PLT08_12375"
     CDS             complement(<1..226)
                     /locus_tag="PLT08_12375"
                     /EC_number="3.1.1.31"
                     /inference="COORDINATES: protein motif:HMM:NF013356.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconolactonase"
                     /protein_id="HNE05311.1"
                     /translation="MNIQKFRDGDELSQAATKNFAELANQAIRERGRFLVSLSGGSTP
                     MKLYARLASENIDWTRVHFFWGDERCVPVDD"
     gene            265..1116
                     /locus_tag="PLT08_12380"
     CDS             265..1116
                     /locus_tag="PLT08_12380"
                     /inference="COORDINATES: protein motif:HMM:NF017984.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0036297 - interstrand cross-link repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crosslink repair DNA glycosylase YcaQ family
                     protein"
                     /protein_id="HNE05312.1"
                     /translation="MNQINLKKLQALRTRTFNLPPQKRVSSPASALTFVNKRGFVYFW
                     PIKGIDLPSLWTAVAGDRPVADKHDDPGHITWGWKDDALDKKIWYYAKILRGKATMIS
                     LEIAPYFYALSENYGSPEEDYLIAYQEGRLPQSAKQVYEALLDKGAMNTLDLRKEAKL
                     SNAKDSEFNKALEYLQRDFKILPVGVAQAGAWKYSHIYTITTSHFPELAEQARTITES
                     QARAKLLELYFEMVGAAQIRDVQKLFGWGNEVVKRAVSKMVDAGDLVLAEHPKQTGEW
                     VAVKEAI"
     gene            1218..1907
                     /locus_tag="PLT08_12385"
     CDS             1218..1907
                     /locus_tag="PLT08_12385"
                     /inference="COORDINATES: protein motif:HMM:NF024701.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HNE05313.1"
                     /translation="MVRPSKKQQLPNLQEAIKETAWKQIAEKGAAALSLRAIARELKI
                     TAPAIYNYFPDRDALVTALIIDAYTSFGDSQIAARDAISKEKIEERFTAVGIAYRQWA
                     KTYPQRYQLIFGTPIPGYQAPLMEILPSAARSLSASVSVIDDLRIVNRLNSSGYPNVQ
                     PGYEQQFEVWKKFAGDYDILSLAVSMLVWSRVHGLVSLEIANNMPPFGVDGDSLYMYE
                     LKSIIKQFIKP"
     gene            1959..2399
                     /locus_tag="PLT08_12390"
     CDS             1959..2399
                     /locus_tag="PLT08_12390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE05314.1"
                     /translation="MYTVSITRLRVRSIFFMPLFTYHAMRTSMQVQKSEGIVGADTRF
                     EKNNVVWTKTVWTEESAMKKYRGSGAHQIAMRILSEMCSEASVTRWQQESTELPTWEE
                     AHRRMLTEGKQSKVKHPSALQASGKTAPDTMQSGFKMPTPPVTQ"
     gene            2396..>2742
                     /locus_tag="PLT08_12395"
     CDS             2396..>2742
                     /locus_tag="PLT08_12395"
                     /inference="COORDINATES: protein motif:HMM:NF012770.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HNE05315.1"
                     /translation="MSLAKKWMFNHPVTHRVKDGYFENDGIRQHYVTLGEGKLVVLIH
                     GIPEFWYSWRHQIDALSQQYQVVAIDQRGFNESGKPASTDGYRVERLVEDVAALIRYL
                     GRKQAVIVGHDAGA"
BASE COUNT          727 a          619 c          710 g          686 t
ORIGIN      
        1 ggtcatccac tggaacgcag cgttcgtcgc cccaaaagaa atgcacacga gtccagtcta
       61 tgttttcact ggcaagccgt gcatataatt tcatgggggt gctgccgccc gagagtgaaa
      121 cgaggaaacg tccgcgttcg cgaatggctt gattggctaa ctcagcaaaa ttttttgttg
      181 cggcttggct tagttcgtcg ccatctctaa atttttggat attcataatt ggtcgatttt
      241 atcgcaaatg ataaaatctg agcgatgaat cagattaatt taaagaaact tcaagcgctt
      301 cgcacgcgga ccttcaacct gcctccgcaa aaacgggttt catctcccgc ctcggctttg
      361 accttcgtca acaaacgcgg cttcgtttac ttctggccca tcaaaggcat tgacctgcct
      421 tcgctgtgga cagcggttgc gggcgataga ccggtggcag acaagcacga tgacccaggt
      481 cacatcacct ggggctggaa agacgatgcg ctcgataaaa agatctggta ctacgccaag
      541 attttacgcg gcaaggcgac catgatctca ttggagatcg cgccgtattt ttatgccttg
      601 tctgaaaatt atggttcccc cgaagaagat tacttgatcg cttatcaaga aggacgtttg
      661 ccacaatccg cgaagcaagt gtatgaagct ttactggata aaggggcgat gaatactttg
      721 gacttgcgca aagaggcgaa gttatccaat gccaaagatt ctgaattcaa taaggcattg
      781 gaatatttac aacgcgattt caagatatta ccagtcggcg tggcgcaggc tggcgcgtgg
      841 aagtattctc atatctacac tatcacgacc agtcatttcc ctgaattggc agagcaagca
      901 cggacaatca ccgaatcgca agcgcgcgca aagttattgg aactgtattt tgagatggtt
      961 ggcgcggcgc aaatccgcga tgtgcagaag ttgtttggtt gggggaatga ggtggtgaag
     1021 agggcagtga gtaagatggt agatgcgggt gatttggttc tggcggagca tcccaaacaa
     1081 accggagaat gggtggcagt aaaagaagcg atatagcaaa cctcggaggt tttcaaaacc
     1141 tccgaggtct ttttttaccc ttgacttaat ttactataca ctgtatattt acactgtata
     1201 gtaaataagg agccgaaatg gtcagaccga gcaagaagca acaactccca aaccttcaag
     1261 aagccatcaa agaaactgcg tggaagcaga ttgctgaaaa gggggcggcg gcattatccc
     1321 tacgggcgat tgcacgcgaa ctgaagatca cagcgccggc tatctacaac tacttcccag
     1381 atcgggatgc gctggtgacg gcactcatta ttgatgccta cacttccttt ggcgactctc
     1441 agattgctgc tagagatgcc atctcaaaag aaaagataga agagcgattt actgcggttg
     1501 gcattgccta ccgccagtgg gcgaagacct atccgcagcg gtatcagctt atttttggca
     1561 cgcctattcc gggatatcaa gctccattga tggagatcct gccgtccgca gcgcgttctt
     1621 tgagcgcctc tgtgagcgtc attgatgatt tgcgtatagt caataggtta aattcgagcg
     1681 gatatccaaa tgttcagccc ggctatgagc agcaatttga agtgtggaaa aaatttgcag
     1741 gggattacga cattttgtcg ttggctgtct ctatgttggt gtggagtcgt gttcacggct
     1801 tggtttcttt ggagatcgcg aacaacatgc cgccctttgg tgtggatgga gactcgcttt
     1861 acatgtacga actgaaatcc atcattaagc aatttatcaa accgtaatat tcaaaaatat
     1921 cgccttacct acacaaccaa ataaatatag gagtcatcat gtatacagtt tccattaccc
     1981 gcttgcgagt ccgctcgatc ttttttatgc cactatttac atatcatgcc atgcgtacca
     2041 gcatgcaggt acaaaaatct gaagggattg tcggcgcaga tactcgcttt gaaaaaaata
     2101 acgttgtttg gacaaagacc gtctggacgg aagaatcagc gatgaaaaaa tatcgtggtt
     2161 caggcgcgca tcagatcgcc atgcgtattc tgtctgaaat gtgttctgaa gcttcagtga
     2221 cccgctggca gcaagaaagc acggaactgc ccacatggga agaagcgcat cgccgcatgt
     2281 tgaccgaggg taaacagtct aaggtgaaac atccgtcagc gttgcaggct tccgggaaga
     2341 ccgctccaga caccatgcaa tcgggcttca aaatgcctac tccgccggtg acccaatgag
     2401 tcttgcaaaa aaatggatgt tcaatcaccc tgtcactcat cgcgtgaagg acggatactt
     2461 tgagaacgat ggtattcgtc agcattatgt cacattaggt gaaggcaagt tggtggtgct
     2521 cattcacggc atccctgagt tttggtattc atggcggcat cagatagatg cgttgtcgca
     2581 gcagtatcag gttgtcgcaa tcgaccaacg cggattcaac gagtcgggta aacctgcttc
     2641 cacagacggc tatcgggtgg agaggttggt ggaagatgtt gcagcgttga ttcggtatct
     2701 cggacggaag caggcggtga tcgttggtca tgatgcgggg gc
//
LOCUS       DAOQJE010000496         2739 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_25856_length_2739_cov_5.865499, whole genome shotgun sequence.
ACCESSION   DAOQJE010000496 DAOQJE010000000
VERSION     DAOQJE010000496.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2739)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2739)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2739
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25856_length_2739_cov_5.865499"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..405
                     /locus_tag="PLT08_12400"
     CDS             <1..405
                     /locus_tag="PLT08_12400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal 5'-phosphate synthase lyase subunit
                     PdxS"
                     /protein_id="HNE05316.1"
                     /translation="VRHARTVIGDIKKLTVMGDEELMAFAKNNGAPYELVKEVKELGR
                     LPVVNFAAGGVATPADAALMMQLGVDGVFVGSGIFKSGDPAKRAAAIVKSVTHYQDAS
                     ILAEVSKNLGEAMVGRNVSQMAEGEKIAGRGW"
     gene            453..1031
                     /gene="pdxT"
                     /locus_tag="PLT08_12405"
     CDS             453..1031
                     /gene="pdxT"
                     /locus_tag="PLT08_12405"
                     /EC_number="3.5.1.2"
                     /EC_number="4.3.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA];
                     GO_process: GO:0042823 - pyridoxal phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal 5'-phosphate synthase glutaminase
                     subunit PdxT"
                     /protein_id="HNE05317.1"
                     /translation="MKIGVLALQGDFAEHVSMLKRVGVEAAEVRLPKHLDDLNGLIIP
                     GGESTTIGKLAVAYNLIDPLREFGKSHAIWGTCAGAIFLSNDIGRDQPLLGLMSIKVK
                     RNAFGRQVDSFETDLEIEELYKATKTKHPYHAVFIRAPIIESVSGDARVLSALEDGRI
                     IAAQQGHLLATSFHPELTDDTRFHEYFVSLAS"
     gene            1067..>1275
                     /locus_tag="PLT08_12410"
     CDS             1067..>1275
                     /locus_tag="PLT08_12410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE05318.1"
                     /translation="MTIRPLDLLDLPLIARYRNDVLTLDSTRALTRGHPLGAMGLLAY
                     INPARHLYAAVSDNEGVTLLGGVIHT"
     assembly_gap    1276..1375
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1376..2139
                     /locus_tag="PLT08_12415"
     CDS             <1376..2139
                     /locus_tag="PLT08_12415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE05319.1"
                     /translation="AAVSDNEGVTLLGGVIHTRGEPFAKLLYLAPSQQLDTPQLTALI
                     EHLTAQAGEWQAFHVIAEVDETSEVFIALRKAGFSVYAWQRVWDASLIQKTESNPGTG
                     WSRVKSIDLPAIQRMHYQIVPQLLHLVDPAPKLPNGFIHTDGYCFASVNYGVYGVVLT
                     PWILPDEGDVSEKIVSLIASLPDRRGRPVYLNVRSYQAWLEPVLEDLGAKAAPRQAVM
                     VKYLSRLVKEGVPARVVPSNARIQPTQMSRMDTDE"
     gene            2132..2452
                     /locus_tag="PLT08_12420"
     CDS             2132..2452
                     /locus_tag="PLT08_12420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE05320.1"
                     /translation="MSSWSIYNKGSTIGTLSAEGGLILSDEAHDGGARITLKRGTNYV
                     SVSLHILGWMDHTRFFANDADAQREYRNMRSGVLTVLDVINTEGVSDIKIWEAISDFV
                     RRFP"
     gene            2454..>2739
                     /locus_tag="PLT08_12425"
     CDS             2454..>2739
                     /locus_tag="PLT08_12425"
                     /inference="COORDINATES: protein motif:HMM:NF024811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase-like protein"
                     /protein_id="HNE05321.1"
                     /translation="MLKALIFDFDGLILDTETPEVLVWQDIYKEHGFELPVEEWEKTV
                     GGYGISSFDPAHHLSHLSQGRLDPVSLKARYRLEADAIIHTKPIQPGIMDM"
BASE COUNT          624 a          603 c          733 g          679 t
ORIGIN      
        1 gtgcgccatg cccgcactgt gatcggtgat atcaagaagt tgaccgtgat gggcgatgaa
       61 gaattaatgg catttgccaa gaacaacggc gctccgtatg aattggtgaa agaagtgaag
      121 gaacttggtc gtttgccggt ggtgaacttt gctgcgggcg gcgtggcgac ccctgccgat
      181 gccgcgttga tgatgcagct cggtgtggac ggcgtctttg ttggctcggg tatcttcaaa
      241 tctggtgacc ctgccaagcg tgcggcagcg atcgtcaaat cggtgacgca ttatcaagat
      301 gcttccattc ttgcggaagt gagcaagaac ctcggcgaag ccatggtcgg acgtaacgtc
      361 tcgcagatgg cagaaggcga aaaaatcgcc ggacgcggtt ggtaaattta ttttggtagg
      421 ggcggggtta ccccgcccct acattgaatg atatgaaaat cggtgtttta gcgttacaag
      481 gtgattttgc agaacatgtt tctatgctga agagagtcgg tgtagaagct gcggaagtgc
      541 gcctgccaaa gcatttggat gatttgaacg ggctcatcat ccctggcggc gagtcgacga
      601 cgattggcaa attggcagtg gcgtataact tgatcgaccc gttgcgcgag tttggaaagt
      661 cacatgcgat ttggggaact tgcgcgggtg ccatctttct ctcgaatgat attggacgtg
      721 accaaccttt gctcggtttg atgagtatca aagtgaagcg caatgcgttt ggacggcaag
      781 tcgattcgtt cgagaccgac cttgagatcg aagagttgta taaagccacc aaaacaaagc
      841 atccttatca tgcggtattc atccgtgcgc cgatcattga atcggtgagc ggagatgcca
      901 gagtgctttc tgccttggaa gatgggcgca tcattgcagc acaacagggg catttgctgg
      961 caacatcctt ccaccccgag ttgaccgacg atacgcgctt ccatgagtat tttgtctcat
     1021 tagcatcgtg atatatttgt aggggcgcaa ccttcgcgcc cctactatga ctattcgtcc
     1081 actcgacctg ttagacctgc ccctcattgc tcgttatcga aacgatgttt tgactctcga
     1141 tagcacgcgt gccctcacac gcggacatcc cctcggtgcg atgggattgt tggcgtatat
     1201 caacccggcg cggcatttgt atgcggcggt cagtgataac gaaggtgtga ccttattagg
     1261 cggagtgatt cacacnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1321 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnatgcg
     1381 gcggtcagtg ataacgaagg tgtgacctta ttaggcggag tgattcacac tcgtggtgaa
     1441 ccttttgcca aattgttata tcttgcacct tcacagcaac ttgatactcc gcaacttaca
     1501 gcgttgatcg aacatctcac tgcacaggct ggggagtggc aggcatttca tgtcatcgca
     1561 gaagtggatg agaccagcga ggttttcatt gcactgcgga aagcaggctt ctccgtctat
     1621 gcatggcaaa gggtttggga tgcgagtctg attcagaaaa cagaatcaaa ccctggcacg
     1681 ggatggagcc gggtcaaatc catagacctg cctgctattc aacgcatgca ttatcagatc
     1741 gtcccacagt tacttcatct tgttgacccg gcaccgaaac tgccgaacgg atttattcac
     1801 accgatggat attgttttgc gagcgtcaat tatggtgtgt atggcgtggt gctcaccccg
     1861 tggattcttc cggatgaagg cgatgtgagc gaaaaaattg tttcactgat cgcaagcctg
     1921 ccagatcggc gcgggcgccc ggtgtatttg aatgtgagat cgtatcaggc ttggcttgag
     1981 cctgtgttgg aagaccttgg tgcaaaggct gcgccgcggc aagccgtaat ggtgaaatat
     2041 ttatcgcgct tggttaaaga gggtgtgcca gctcgtgtgg tgccctcgaa tgcgcgcatt
     2101 caacccaccc aaatgagtag aatggatacg gatgagtagt tggtcgattt ataacaaagg
     2161 tagcaccatt ggcacactta gtgctgaagg tggtttgatt ctttcagatg aagcgcatga
     2221 tggcggcgca cggattacgc ttaagcgtgg tacgaattat gtttcagttt cacttcacat
     2281 tcttggttgg atggatcaca cccgattttt tgctaatgat gccgatgcgc aacgtgaata
     2341 tcgcaacatg cgttcgggcg ttctcaccgt gttggatgtg atcaacacgg aaggtgtaag
     2401 cgatatcaag atttgggaag caatttcaga ttttgtcagg cggtttccat aatatgttga
     2461 aagcactcat atttgatttc gacgggttga ttctcgatac cgaaacaccc gaagttttag
     2521 tttggcagga catttacaaa gaacatggat ttgaacttcc cgtggaagaa tgggaaaaaa
     2581 ccgtgggcgg atacggcatt tcaagctttg accccgccca ccatctttcg catctttctc
     2641 agggacggct ggatcctgtt tccctcaaag ctcgctacag gctggaagcg gatgcgatca
     2701 tccacaccaa gccaatccaa ccgggcatca tggatatga
//
LOCUS       DAOQJE010000497         2734 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_25962_length_2734_cov_2.801045, whole genome shotgun sequence.
ACCESSION   DAOQJE010000497 DAOQJE010000000
VERSION     DAOQJE010000497.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2734)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2734)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2734
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25962_length_2734_cov_2.801045"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..323
                     /locus_tag="PLT08_12430"
     CDS             <1..323
                     /locus_tag="PLT08_12430"
                     /inference="COORDINATES: protein motif:HMM:NF014799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transketolase C-terminal domain-containing
                     protein"
                     /protein_id="HNE05322.1"
                     /translation="ASELLAERGFGLSIINMPWLNRVDVKWLTEVLKPYSNIFMLEDH
                     HPAGGLMDNILTVCSEHGLLNGKQMIKFAVEGYPACGTPQQALAFHELDGLSLSKRVM
                     KYCG"
     gene            327..1736
                     /locus_tag="PLT08_12435"
     CDS             327..1736
                     /locus_tag="PLT08_12435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE05323.1"
                     /translation="MSEIKIGIPLHGKMAARAFHQPSFRQRLAEGGFKPLYFLSPTYF
                     RAFDFDREQYFELQVEQYDEQYQKHFLLQQLRMLRRFVVVTETTDLRFRELIESKLFD
                     ATLVGMAAQMSFVAALRHIPGMGGVLGWLERNLYVTHAHDEKFNKHQITCILTPGMGN
                     YGFWNENQFAHEAQRLGIPTFTAITNYDNIVNMGYRGFEPVCLGVWSKQMADEAMKLH
                     GYPANKMEITGPVQYDRFMMPLPQTREEFLKSLNLDPSKKTILFAGGVNINHYFDIYR
                     VFVEQKAKVWREPFNFIVRPYPHPKLMGSPAWAVLENLFKQSGAYISNPGSIDASGDR
                     TAEFRVDLAFDEGPDELNYLLRYSDVMVNYFSTISLEAAICDLPTIHIGFDPYTFGLR
                     FSVTTGFLQRQTHNRRPLRLAAARVAKSESELFDALENYLGDRTLDRDARRIYAEAEC
                     GELDGGAGRRLVNMIKNRI"
     gene            1778..>2734
                     /locus_tag="PLT08_12440"
     CDS             1778..>2734
                     /locus_tag="PLT08_12440"
                     /inference="COORDINATES: protein motif:HMM:NF014589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyltransferase N-terminal
                     domain-containing protein"
                     /protein_id="HNE05324.1"
                     /translation="MKSILGVSLGHDTSFAHIVNGKVVAVMEAERYFRQKRYKLHCLT
                     MEPGKHISGYQYVSLDDIELFLGMVAKEWGTSFDALAVQNQGRVEEFKNFQQILEKLG
                     FKFGSVHHVDHHLSHAALAFFSSSFKDALVLSYDGEGNDGQTIIFGAKGNTIEYLHND
                     PVRYGQSYNNMGYIVGIKPEVEGTTSGKVMGLTAYGEPVQDWLPYARDYIKKYVKATP
                     RQIEGLNNYGKAHRINDIGVRDIPDLKKFLVPEDEKNTGLKAAVKKATGIKNTVLRLN
                     GPEDKNAQNFAATVQAAWTESVIDLLKPYFGKYENLCVVGGCA"
BASE COUNT          722 a          601 c          669 g          742 t
ORIGIN      
        1 tcgccagcga actcctcgcc gagcgcggat tcggcttgag catcatcaac atgccctggc
       61 tcaaccgcgt ggatgtgaag tggctgaccg aagttctcaa gccgtattcc aatatcttca
      121 tgctcgaaga tcatcacccc gctggcggct tgatggataa catccttacg gtgtgcagtg
      181 aacatggttt attgaatgga aagcagatga ttaagtttgc ggtagaaggc tatcccgcct
      241 gtggcacgcc gcaacaggcg ctggcatttc atgaattgga tggtttgtcc ttgtccaagc
      301 gtgtgatgaa gtattgcggt taatgaatgt ctgaaataaa gatcggcatt cctttgcacg
      361 gcaagatggc ggcgcgcgcc ttccatcaac catcctttcg ccagcgtttg gcggagggtg
      421 gttttaagcc gttatatttt ctcagcccta cctatttccg cgcatttgat tttgaccggg
      481 aacaatactt tgaattgcaa gttgaacaat acgacgagca atatcaaaaa cattttttgc
      541 tgcaacaact gcggatgttg cgccgctttg tcgttgtgac cgagaccacg gatctgcgtt
      601 ttcgagaatt gatcgaaagc aaattgtttg acgccaccct ggtaggtatg gcggcgcaaa
      661 tgagcttcgt cgcggcgctg cgtcatattc ccgggatggg tggggtgtta gggtggcttg
      721 aacgaaacct ttatgtaacc catgcacacg acgaaaaatt caataagcat caaataacct
      781 gtatcttgac gccgggaatg ggaaattatg gcttctggaa tgaaaaccag tttgcgcatg
      841 aggcacagcg cttgggtatt cccaccttta ccgccatcac caactacgac aatattgtca
      901 acatgggcta tcgtggtttt gaaccagttt gtttaggcgt atggagcaaa caaatggcag
      961 atgaagccat gaagttgcat ggttatccgg caaacaaaat ggagatcacc ggacccgtgc
     1021 aatatgaccg tttcatgatg cctttgcctc agacccgcga agaatttctt aagtcgctca
     1081 accttgatcc atctaaaaag accatcctct ttgccggtgg cgtcaatatc aatcattact
     1141 tcgatattta tcgggttttc gtagagcaga aggctaaggt ctggcgcgaa ccgttcaact
     1201 tcatcgtccg cccgtatccg caccccaagt tgatgggttc ccctgcctgg gcggttttgg
     1261 aaaatctctt taaacagtct ggtgcgtata tttccaaccc tggctccatc gatgccagtg
     1321 gagatagaac cgccgagttc cgcgttgacc tcgccttcga tgaaggaccc gatgagttga
     1381 attacctgct ccgttacagt gatgtaatgg taaattactt ctccaccata agccttgaag
     1441 ccgcgatatg cgatctgcct accattcaca ttggtttcga tccatacacg ttcggtttgc
     1501 gcttcagcgt cacaaccggg tttcttcaac gtcaaacgca taatcggcgt cccttgagac
     1561 ttgctgcagc tcgtgtggca aaaagtgaat cagaactctt tgatgcgctt gagaattatc
     1621 ttggtgaccg tactcttgac cgtgatgcac gtcgaattta tgctgaggct gaatgtggag
     1681 agttggacgg tggggcaggc agacgcttag tcaatatgat taaaaacaga atctagtatg
     1741 gttatcatca aaactcaatc atatcctgga ggataaaatg aaaagtattt taggtgtaag
     1801 tttaggacac gatacgagct ttgcacacat tgtgaatggc aaagttgttg ccgttatgga
     1861 ggctgaacgt tattttcgtc agaagcgcta taaattacat tgcctgacaa tggaacctgg
     1921 taaacatatc agtggatatc aatatgtttc tctggatgat attgaactat tccttggtat
     1981 ggtggcaaaa gaatggggta catcgtttga tgcccttgcc gttcaaaatc agggacgtgt
     2041 ggaagagttc aagaactttc agcaaattct tgaaaaactg ggatttaaat ttggatctgt
     2101 tcatcatgta gatcatcact tgagtcatgc tgcactggct tttttctcat cttcattcaa
     2161 agatgctctt gtccttagct atgatggtga aggcaatgac gggcaaacca ttatttttgg
     2221 cgcaaaaggt aatacgatcg aatacttgca caatgatcct gttcgatatg ggcagagtta
     2281 taacaatatg gggtacattg tcggaattaa acctgaggtg gaaggaacta catctggtaa
     2341 ggtgatgggt ctcactgcct atggtgaacc cgtgcaggat tggctgccct atgcccgaga
     2401 ctatatcaag aagtatgtca aggcaactcc ccgacagatc gaaggactca ataactacgg
     2461 caaggcgcat cgcatcaatg atattggcgt ccgtgatatt cccgacctca aaaaatttct
     2521 tgttccagaa gatgaaaaga ataccggttt gaaggcagcg gtcaaaaaag cgacaggtat
     2581 caagaatacg gttctgcgtc taaatggacc ggaagataaa aatgcacaaa attttgccgc
     2641 caccgttcaa gcagcatgga ctgagtctgt gattgacttg ctcaaaccct attttggaaa
     2701 gtatgaaaac ctttgtgtag tgggtggatg tgcc
//
LOCUS       DAOQJE010000498         2729 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_26020_length_2729_cov_8.062453, whole genome shotgun sequence.
ACCESSION   DAOQJE010000498 DAOQJE010000000
VERSION     DAOQJE010000498.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2729)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2729)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2729
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26020_length_2729_cov_8.062453"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            290..904
                     /locus_tag="PLT08_12445"
     CDS             290..904
                     /locus_tag="PLT08_12445"
                     /inference="COORDINATES: protein motif:HMM:NF012905.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HNE05325.1"
                     /translation="MTIRAVVFDIGNVLEINSETGWQEKWEKSLQLDLGEVFKHLVSM
                     GKDGSLGTCTEEEWAQGLQEVTGMSQTQTNEFMNDLWHWYLGTLNEELAVFFAGLRPH
                     YQTAILSNSFVGARTKEQEKYHFHEITDQIIYSHEVGLAKPDKRIYELTCERLNMQPE
                     EIIFVDDAEPNIVAALEYGIHGILFKNNTQVIADIQTCISANVS"
     gene            1026..1568
                     /locus_tag="PLT08_12450"
     CDS             1026..1568
                     /locus_tag="PLT08_12450"
                     /EC_number="2.1.1.100"
                     /EC_number="2.1.1.334"
                     /inference="COORDINATES: protein motif:HMM:NF018649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenylcysteine carboxylmethyltransferase
                     family protein"
                     /protein_id="HNE05326.1"
                     /translation="MNRERKLILGYLVGGFLVMIFVPSIIYAITSLLDNVYKLEIIRN
                     EAIRWIVFVILLTAGFVYGIWSVIIQNTIGEGGPMEIGSLEISPKTRNLVVSGPYKYT
                     RNPMLFGTFLIYLAFAFFINSITAIVLISAIFIFMLTVVVKMEEQRLLKDFGKQYEDY
                     RKRVSMFIPWFHRKIKSHLD"
     gene            1626..2279
                     /locus_tag="PLT08_12455"
     CDS             1626..2279
                     /locus_tag="PLT08_12455"
                     /inference="COORDINATES: protein motif:HMM:NF017771.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF899 domain-containing protein"
                     /protein_id="HNE05327.1"
                     /translation="MATHNSAHPQIVSRDEWLTARETLLAHEKELTKHQDKISAERRR
                     LPMVKVEKAYIFNGSEGRRSLYDLFNGYTQLIVYHFMFDPSWDNGCMGCTGFVNDLGD
                     LSELQVRNTNFVIISRAPLEKLLAYKTRMGWNRPWYSAFNTDFNQDFQVTVDGGERHG
                     LSVFFRIDDDIYHTYSTYKRGCESLSDVYRLLDMTPYGRQEDWEDSPAGFPQRPTYG"
BASE COUNT          837 a          557 c          571 g          764 t
ORIGIN      
        1 aaaagacctg gcaagttacg caaaaggtag tggctcgacg cgttagccaa gtcgaatcag
       61 aatcatctca agtaactccc atgcatcctg aatccagtga ctgaagcgcc gccgaacaaa
      121 gcgtgcactg gattggcggg attctgcgcg atttataagc atttttctgg cttctggctt
      181 ttcctgctct caagcatttt ccctgcccgc cccagcgcag gtaacgcaaa ccgttgggcg
      241 ctggctcgca cagtaaaatt aattaacgtt gatggtcaaa gaggcgaaaa tgacaattcg
      301 tgcagtagtt tttgatattg gtaacgtact tgaaatcaac tcagaaactg gatggcagga
      361 aaaatgggaa aaaagccttc aacttgattt aggcgaagtt tttaaacacc ttgtttcaat
      421 gggaaaagac ggctctcttg gcacatgtac tgaagaagaa tgggctcaag gtttgcaaga
      481 agtaacaggc atgagtcaga cacagactaa tgaatttatg aacgacctgt ggcattggta
      541 tctcggcaca ctgaacgaag aattagcagt tttctttgca ggtttacgtc ctcattatca
      601 aactgcaatc ctcagcaaca gttttgttgg cgcacgcaca aaagaacaag aaaaatatca
      661 ctttcacgaa atcaccgacc aaatcatcta ctctcatgaa gttggattag ccaaaccaga
      721 taagcgtatt tacgaactta catgcgaaag attaaacatg caaccagaag aaatcatttt
      781 tgttgatgat gctgaaccca atatagtagc cgctcttgag tatggtattc atgggattct
      841 tttcaaaaat aatacccaag tcattgcaga tattcaaacc tgtatatcag caaatgtctc
      901 gtaatcccaa aacgcgccca acaaagcgtg cagcggacgg cgggggcaat tgtaaaattc
      961 atgcgtgatt ttctgagcgt tttcatgcat aaatagacct tggcaatgac aggaaggaag
     1021 ctttcatgaa cagggagaga aagttgatcc taggatatct ggtgggtgga ttcctcgtta
     1081 tgatcttcgt gccttctatt atttatgcga tcacttcatt acttgataat gtctataagt
     1141 tggaaatcat ccgaaatgaa gcaattagat ggattgtttt cgtaatcctg ctgacggctg
     1201 gattcgtgta tggaatttgg tcagtgatca ttcaaaacac tattggagaa ggaggtccta
     1261 tggagatagg aagcctggaa atcagtccaa aaaccaggaa tctcgttgtt tcagggcctt
     1321 ataaatacac aagaaacccc atgttatttg gaaccttcct tatctatcta gcatttgcgt
     1381 tctttattaa ttcaataact gcaatcgtgt tgattagtgc aatttttatc tttatgttga
     1441 cagttgttgt aaaaatggag gagcaaagat tattaaaaga ttttggaaaa cagtatgaag
     1501 attatcgaaa aagggtatca atgttcattc catggtttca cagaaaaatc aaatcccatc
     1561 ttgactaagc agaacatata ttctaaaatt ccttctatcg taatttagtc aagaaaggac
     1621 aaaatatggc aacgcacaac tctgcacacc cgcaaattgt aagtagagat gaatggctaa
     1681 cggctcgaga aacattgctt gcccatgaaa aggaactcac caagcatcaa gacaaaataa
     1741 gtgccgaacg tcgcagactg cccatggtaa aagtcgaaaa agcatatatt tttaatggtt
     1801 cggaaggaag acgtagcctt tacgatttat tcaacggata tacccaactc attgtgtacc
     1861 acttcatgtt cgacccttct tgggataatg gttgtatggg ctgcactggt tttgtaaacg
     1921 atctgggaga tttatctgaa ttacaagttc gtaacacaaa ttttgtaatt atctcgcgcg
     1981 ccccattaga aaagctcctc gcatacaaaa cgcgtatggg ctggaatcgt ccctggtatt
     2041 cagcatttaa caccgatttc aatcaggatt tccaagttac agttgatggt ggtgaaaggc
     2101 atggattaag tgttttcttc cgtattgatg atgacatata tcatacatac tcaacttaca
     2161 aacgcggctg tgaatcgtta agtgatgttt atagattact tgatatgact ccctacggac
     2221 gacaggaaga ttgggaagat tctcccgctg gttttccaca gcgcccaacc tacgggtaag
     2281 atttaccgca ggttcaaaac tctacagcga agcgcgcctt gacgctgggg attcagcaac
     2341 aatttcaggc atttttccac accatcgact ttttctggat ggaaagcttt gccgtccctg
     2401 cctcagcgcg gataacgcaa acctttggac tgctctttac atattatcaa tgaatggcag
     2461 atagcacttc accactaaag gaaaatgaac caatgacttc aaggttgcat atcaaagcca
     2521 tactacaagc attatttgta acttttctat ggtcaacgtc atgggtactt gtcaaaattg
     2581 gattgaaagc cagcttaccc gtcattactt tcgcaggctt gcgttatttc actgcgcctc
     2641 tttgcttgat ctgattttta taatcaaccc aacacatcac ctattatgag tggagaggta
     2701 aatgaggaag aacatgatca ccattgagt
//
LOCUS       DAOQJE010000499         2729 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_26022_length_2729_cov_6.562453, whole genome shotgun sequence.
ACCESSION   DAOQJE010000499 DAOQJE010000000
VERSION     DAOQJE010000499.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2729)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2729)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2729
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26022_length_2729_cov_6.562453"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            132..593
                     /locus_tag="PLT08_12460"
     CDS             132..593
                     /locus_tag="PLT08_12460"
                     /inference="COORDINATES: protein motif:HMM:NF019928.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC domain-containing protein"
                     /protein_id="HNE05328.1"
                     /translation="MTSNASLNNSIIFQKSVEIAATTSKVWMLLTEPEQMKKWMMPDI
                     ELSIQTDWEVGCPIFIRGTMSGKEFENKGLVLKFEPEKNLQYSHLSSVSHLPDRAENY
                     SVLDFQLHSINQKTQLMFTLSNFPTESIYKHMAFYWNVTLEVFKRIAEEQR"
     gene            617..1891
                     /locus_tag="PLT08_12465"
     CDS             617..1891
                     /locus_tag="PLT08_12465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE05329.1"
                     /translation="MAQRILRGLEITILLFVLAALTGYSNPSLTNPIEKVRAYTRSLE
                     FDYVEWMANAAVLKMRAASINLPQTLNREEQKQVVMEYFKTTQFILEKEYLLSQLYAD
                     PSIQDKDAATETVRADLAELYKRQEQIAPLAEAILQDQVTQVLVEQGLTVGGQPIPNV
                     WYHSTPLPMALVISPRDHIEQTANISVNTDLTVDEQVKLEEDVAKELDVSSLVVQIGG
                     VGVYPTMVARTTNLNWLLSTIAHEWIHNYLTQRPLGLLYGESPELRTMNETTASIAGD
                     EIGAIVIEKFYPELKPTASFPNLSLISLSFDRPDPETLERPPFDFRAQMHETRVTADA
                     LLAAGKIDEAEAYMEARRQIFLQNGYLLRKINQAYFAFHGAYADVPGGAAGEDPVGPA
                     VRALREQSPSLKDFINTIAWMWSFEQLQETIK"
     gene            1894..>2729
                     /locus_tag="PLT08_12470"
     CDS             1894..>2729
                     /locus_tag="PLT08_12470"
                     /inference="COORDINATES: protein motif:HMM:NF024853.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HNE05330.1"
                     /translation="MKRILFGLVFFGITLFFTACSTENTPTPYIVLDPVIPPTPTPQY
                     TCTVISAMPTLSPNEQSLAPAITAADFSFGPSDAPVTILEYCDFQSDGCLAMAQTIAE
                     LMRNYENQIRFVFRPLPLSTVLDKSDLSALAAFAAGEQGKFWEFYDLLFSKHGEWKNL
                     SPGEFKGWLTRETPAIGIDASQLTAAMEAEGALTQLKSAQEAMTQLYITAVPVILING
                     SLQQSYVLDYRNMYDTVGLILLGEKQFTECPPYTVDASKQYIATIETAKGNIVIQLFP
                     DKA"
BASE COUNT          770 a          740 c          569 g          650 t
ORIGIN      
        1 gaacaagaag tattaaatct tggcttcaac aaatcccggc tagacaaagt aaattgggaa
       61 gagtttccgt cctcagatgt tttcaacctg gcagatgtag cattcatagc cgatgattgg
      121 cgataataaa aatgacatcg aacgcctcac ttaataactc aatcatcttt caaaagtctg
      181 ttgaaattgc agcaaccaca tccaaagtgt ggatgctgct gaccgagccc gaacagatga
      241 aaaaatggat gatgccagat attgagctct ccatccaaac cgattgggaa gtcggctgcc
      301 ctattttcat tcgcggcaca atgagcggaa aagagttcga aaataaaggg cttgtcctaa
      361 aatttgagcc tgagaaaaat cttcaatata gtcacctcag ttcagtatcc catcttccag
      421 accgggcaga aaactactct gttctcgact tccaactaca ctctatcaac caaaaaacac
      481 aattaatgtt caccctgagc aacttcccaa cagagtccat ttataaacat atggcatttt
      541 actggaatgt gacccttgag gtgttcaaaa gaatcgccga agaacagaga taagcgaact
      601 gggtataatc caccctatgg ctcaacgaat tttacgcggg ctcgagatca cgatcctgct
      661 ctttgtcctt gccgcgctta ctggctacag caacccctcc ctcaccaatc cgatcgaaaa
      721 ggtccgcgca tatacgcgca gccttgaatt tgattatgtg gaatggatgg caaatgccgc
      781 cgtgctaaaa atgcgcgccg cttccataaa cctgccgcaa acactcaacc gcgaagagca
      841 aaaacaggtc gtaatggaat atttcaaaac gacccaattt attcttgaaa aagaatattt
      901 gctctcacag ctttatgcag acccatccat tcaagacaaa gatgccgcta cagaaactgt
      961 ccgtgcagac ttagcggaac tgtacaaacg tcaggaacaa atcgcaccat tggcagaagc
     1021 catcttgcaa gatcaagtca cacaagtctt ggtcgaacaa ggtcttaccg tgggcgggca
     1081 acccattcca aatgtctggt atcactccac tcccctgccc atggcacttg tcatctcgcc
     1141 acgcgaccat atcgagcaga ccgccaatat ttcagtcaac acagacctca cagttgatga
     1201 acaagtcaaa cttgaagaag atgttgcaaa ggagttagat gtctcgtcgc ttgttgtgca
     1261 aatcggcggc gtaggcgtat accccaccat ggtagcacgc accaccaacc tcaactggct
     1321 gctcagcact atcgcccacg aatggatcca caactacctc acgcaacgtc cacttgggtt
     1381 gttatatggc gaatctcccg aattgcgcac catgaacgaa acgacagcat cgattgctgg
     1441 cgatgaaatt ggagcaatag tcatcgaaaa gttttacccg gaattaaaac ccaccgcctc
     1501 tttcccaaac ttgagcttga tctcgctttc ctttgaccgc cccgaccccg aaaccttaga
     1561 gcgcccgccc ttcgacttcc gcgcgcaaat gcacgaaacc cgcgtcactg ccgatgccct
     1621 actagcagcc ggaaaaatcg acgaagcaga agcctacatg gaagcgcgcc gtcaaatctt
     1681 tttacagaac ggctatctcc ttcgtaagat caaccaagcc tacttcgcct tccacggcgc
     1741 ctatgcagac gtgcccggtg gcgccgccgg tgaagacccg gttggtcccg ccgtgcgtgc
     1801 cttgcgcgaa caaagcccct cactcaaaga tttcatcaac accatcgctt ggatgtggtc
     1861 tttcgaacaa ttacaggaaa ctatcaaata ataatgaaac gaattctctt cgggcttgta
     1921 ttctttggca taaccttgtt tttcacggca tgtagcacag agaatactcc tacaccttat
     1981 atcgtgctcg atcctgttat tccgccaacg cctacgcctc aatatacctg cacggtgatc
     2041 agtgccatgc ctacgctctc cccaaatgag cagtctctcg cgcctgccat caccgcagca
     2101 gatttttcct ttggtcccag tgatgctcca gtaactattc tagaatattg cgattttcag
     2161 tctgatggct gccttgccat ggcacagacc attgccgagc tcatgcgcaa ttacgagaat
     2221 cagattcgct tcgtgttccg tccgctgcca cttagcaccg tgttggataa atcagacctt
     2281 tccgcactgg cagcctttgc cgctggagaa caaggcaagt tttgggaatt ttatgatcta
     2341 ttattttcaa aacatggcga gtggaaaaat ctaagcccgg gcgaattcaa aggctggctc
     2401 acaagagaaa ctcccgctat tggcattgat gcgagtcagc ttacagcggc gatggaagcc
     2461 gaaggcgcat tgacccagct caaatcggcg caagaggcga tgactcagtt atacatcacc
     2521 gcggttccag tcatcctcat caatgggtcg ttacagcaat cctatgtttt ggattatcgc
     2581 aatatgtacg ataccgtagg gctaattttg cttggcgaga aacagtttac cgaatgccca
     2641 ccttatacgg tagatgcatc gaaacaatac attgccacga ttgaaactgc caaaggaaat
     2701 attgtgattc aattatttcc agataaagc
//
LOCUS       DAOQJE010000500         2728 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_26044_length_2728_cov_8.523382, whole genome shotgun sequence.
ACCESSION   DAOQJE010000500 DAOQJE010000000
VERSION     DAOQJE010000500.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2728)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2728)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2728
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26044_length_2728_cov_8.523382"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(86..541)
                     /locus_tag="PLT08_12475"
     CDS             complement(86..541)
                     /locus_tag="PLT08_12475"
                     /inference="COORDINATES: protein motif:HMM:NF019013.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030638 - polyketide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ester cyclase"
                     /protein_id="HNE05331.1"
                     /translation="MKAYDVVNQHLRAVEAGDWEKADSFIADDYSMSGTIPFPVSLFI
                     KIGKKESLRMHKPRKTALPDFKFNEKILEETEEMVSFQVNLTGTHTGVIDYTGILRGV
                     PVIQPTGKKVELPDEFFTYFVRAGKIYKTIGRIPKNAGVQALVRAVTGS"
     gene            complement(604..1008)
                     /locus_tag="PLT08_12480"
     CDS             complement(604..1008)
                     /locus_tag="PLT08_12480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE05332.1"
                     /translation="MKYLIYVFYFEILINFASGGQSLFMPAAFLGQFSSEPAPAFALE
                     MTRWYGVLIAVLTYLLIRGLMLRGAALKVALEALLFGDVIQIIVSFVTANALGGWTSN
                     VILSVVVSVILGAVRLICLWKPVETGIERSTP"
     gene            1154..>2728
                     /locus_tag="PLT08_12485"
     CDS             1154..>2728
                     /locus_tag="PLT08_12485"
                     /EC_number="4.1.1.31"
                     /inference="COORDINATES: protein motif:HMM:NF012532.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008964 - phosphoenolpyruvate carboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA];
                     GO_process: GO:0015977 - carbon fixation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxylase"
                     /protein_id="HNE05333.1"
                     /translation="MEISQNIRLLGNLLGKVISEMESPELFDIEERIRALAKARRNGD
                     ADAALKLQQAVSGLKDADARVVAAAFTAYFDLVNLAEEDHRVQMLRKQEQDSFPAPAH
                     ESIGEAIEVLKRNGMTSDEMNALMQSLSIELVLTAHPTEARRRTILSKTERIAAQLDL
                     IARNGLYSYEKQNAHNALSAAILALWLTDRTRADKLTVTDEVRTGLYFVESYFWNTLP
                     ALYHDLELALATHYPDVKPPHHWLKLASWIGGDRDGNPFVKTDITAETLRLHRGLAVE
                     GLRREFQELGRQLSVSSSRILPPPELLEWIEKRRPFPKRVEYIEKRYANEPYRLAIAL
                     LSNDLAEASKEEMTTHLLGTHEHQARLDIREVLHVLSLIASVLPESLVQDQLQAVIHK
                     AQIFGLRAARLDIREDSSRFNSALSEVLRALNIEPNFESLPDDARTELLERLLRDPVP
                     SLAPSLGVTAAAAETWALFKLIGRTNEIYGSDLLGPIVISMTHAAADVLTVLLLAKWG
                     GCKTLPSITPLFEVIQD"
BASE COUNT          696 a          671 c          649 g          712 t
ORIGIN      
        1 cagctatacg agacgcagtt ccgagcggaa cgggtgtagc tttttcaaga aaaaatccca
       61 aaaccatggt tttgggattt tttaattatg agcctgttac tgcccgcacc agagcttgaa
      121 ctcctgcgtt ctttggaatt cttccaatag ttttgtatat ctttccagcg cggacgaagt
      181 atgtaaagaa ttcatctggt agctctactt ttttgccggt gggttgaatc acgggcacac
      241 cacgtaaaat gccggtgtaa tcaatcacac cggtatgcgt tccagtgagg ttgacctgaa
      301 aactcaccat ttcttcggtc tcttccagaa tcttctcgtt gaatttgaaa tcaggcaggg
      361 cggttttacg cggtttgtgc atgcgcaggg attctttttt gccgatctta atgaacaaac
      421 tgacggggaa tggaatggtt ccgctcatcg aataatcgtc tgcgatgaac gagtcggctt
      481 tttcccaatc gcctgcctcc acagctctta agtgctggtt gactacatca taagctttca
      541 ttgatctctc ctttattcta aagacacaat actgattgat ctctgaaaga ttttcttacc
      601 aagctatggt gttgatcttt caatgccagt ttcaactggc ttccataaac agattaaacg
      661 cactgcaccc aaaataacgg aaacaaccac cgataagatc acatttgagg tccagccgcc
      721 gagtgcgttg gcagtgacaa acgaaacgat gatttgaatc acatcgccga agagtagggc
      781 ttcgagagcc acttttagcg ctgccccgcg cagcatcaac ccgcggatga ggaggtaggt
      841 taacaccgca ataagcacac cataccaacg cgtcatttca agggcaaagg cgggcgcagg
      901 ctcgctggag aattgaccga gaaaggcggc aggcataaaa agggattgtc cgccactggc
      961 gaagttgata aggatttcaa agtaaaacac atagataagg tatttcatgg tcggctcctg
     1021 agtgggtgag tatttgaatg cgattatata actgttgcag cttggtacat cttgaaaatg
     1081 acttgtcgct atcacaaaga attccccaaa tgggtattgt gagcatacct cacttgctat
     1141 acaatcaaaa gttatggaaa tttctcaaaa cattcgcttg ttgggcaatt tgctcggtaa
     1201 agttatttca gaaatggagt cgccggaact tttcgatatt gaagagcgca tccgtgcact
     1261 tgccaaagcg cgtcgaaatg gcgatgcaga cgccgccctg aaattacagc aagccgtttc
     1321 aggcttgaaa gatgccgatg cacgcgtagt ggcggcagcc ttcactgctt acttcgacct
     1381 cgtgaacttg gcagaagaag accatcgtgt gcagatgctt cgcaagcagg aacaggattc
     1441 tttccccgca cccgctcacg agtccattgg tgaagcgatt gaggtcctca agcgaaacgg
     1501 catgacctcc gatgagatga atgctctcat gcagagtctt tccatcgagc tggttcttac
     1561 tgcacatcct actgaagctc gccgtcgtac catcctctca aagacagagc gtatcgcagc
     1621 ccagctagat ttgatcgcca gaaatgggtt gtattcatat gaaaaacaaa atgcccataa
     1681 cgcgctgtct gctgccatcc ttgcactctg gcttacagac cgcacccgcg ccgataaatt
     1741 aaccgtcaca gatgaagtgc gcactggttt gtactttgtt gaatcgtatt tttggaacac
     1801 cttgccggct ctttatcatg atcttgaact tgcactggca actcattacc ccgatgtaaa
     1861 gccgccacac cattggctaa aactggcttc gtggatcggc ggcgaccgcg atggaaatcc
     1921 cttcgtcaag accgatatta ctgccgagac cttgcgcctg caccgcggtt tggcagtgga
     1981 agggttgcga cgtgaatttc aagaattggg caggcagtta agcgtcagct ccagccgtat
     2041 tttgccgccg cctgaattgt tggaatggat cgaaaagcgt cgtccatttc cgaaacgtgt
     2101 ggaatacatc gagaagcgat atgcaaatga accctatcgc cttgcgattg ctctgttatc
     2161 caacgacctt gctgaagcct caaaagaaga aatgacaact catttgctgg gcacgcatga
     2221 gcatcaagcg cgcttggata ttcgcgaggt cttgcacgtt ttatctttga tcgcatccgt
     2281 cttgccagaa agtttggtgc aagaccaatt gcaagccgtc attcacaaag cccagatctt
     2341 tggtctgcgc gccgctcgtt tggatattcg cgaagactca agccgtttca attcagcatt
     2401 gagcgaagtc ttgcgggcgc tgaacattga acctaatttt gagtcattgc cagatgatgc
     2461 acgcacagaa ttattagagc gcctcttacg ggacccggtt ccgagtctcg ccccgagcct
     2521 tggcgtcact gctgccgctg ctgaaacatg ggcactcttc aaactgatcg gtcgtaccaa
     2581 tgaaatttat ggcagtgact tgttaggacc catcgtcatt tccatgactc atgctgctgc
     2641 cgatgtgttg accgtgcttt tgcttgccaa atggggcggc tgtaaaacat taccgtctat
     2701 tacaccgctt ttcgaagtga ttcaagac
//
LOCUS       DAOQJE010000501         2726 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_26081_length_2726_cov_7.884313, whole genome shotgun sequence.
ACCESSION   DAOQJE010000501 DAOQJE010000000
VERSION     DAOQJE010000501.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2726)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2726)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2726
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26081_length_2726_cov_7.884313"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..618
                     /locus_tag="PLT08_12490"
     CDS             <1..618
                     /locus_tag="PLT08_12490"
                     /inference="COORDINATES: protein motif:HMM:NF014026.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 C-terminal
                     domain-containing protein"
                     /protein_id="HNE05334.1"
                     /translation="EVEDLVEAILFVWYPGMEGGRAVADVLFGDVSPAGKLPITFPKS
                     LDQLPAFDDYGMTGRTYRYMTAEPLYPFGFGLSYTCFEYSNLKLDKTNLALGDSLTLN
                     LTVRNSGGSDSAEVVQYYLSDLHASTIVPLHHLIGFERVHLKAGESKTLTFTITPDMM
                     SFYNDDGKLTLEPGEFRVEVGSCSPGKRGQELGAPAPVTALFEVK"
     gene            639..1523
                     /locus_tag="PLT08_12495"
     CDS             639..1523
                     /locus_tag="PLT08_12495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE05335.1"
                     /translation="METVNFCGQECVVFGNKAIQLLITRSVGPRILSLKFTGGENLLA
                     EMPDFITDCPGSGIYHFYGGHRLWHAPEVPSRTYLPDDSPVDITQIENTLHIVQEVEA
                     KTGLQKSFDIQMQGDCASLSITHRLTNKGLWSATTALWAITQFKPGGLGILPQTVENT
                     GVLPNRRLTFWPYTDPSEPAVHLGKNYILINANMQGAFKVGFSNPRGWQAYWWNGVLF
                     VKRAKYEADAEYFDYGSSSESYCNHEILELETLSPITTIEPGAEATHVETWEMYQGIE
                     RPQNEQDVQALVERLELE"
     gene            1529..>2726
                     /gene="xylB"
                     /locus_tag="PLT08_12500"
     CDS             1529..>2726
                     /gene="xylB"
                     /locus_tag="PLT08_12500"
                     /EC_number="2.7.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004856 - xylulokinase activity [Evidence
                     IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0005997 - xylulose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0042732 - D-xylose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xylulokinase"
                     /protein_id="HNE05336.1"
                     /translation="MNYFLGIDTSTTSSKALLIDSNGVVIAVAAASHTLQTPKPLWSE
                     QNPREWWDAVAASIKSVLEKAGVSGERVVAVGLTGQMHGLILLDENGSVLRPAILWND
                     QRTQSQCDEIHQVIGKEKFIQITGNVALTGFTAPKILWVKENEPSVYARAKHVLLPKD
                     YIRFCLTGEYAMDKADGAGTVLFDLKVRDWSDEVLAALGIPREWMPRTFEGTAFTGKV
                     TEEAARLTGLKAGTPVAAGGGDQAAGAVGVGAVEPGIVGLTVGTSGVVFATTPSALIE
                     PEGRLHAFCHAVPGMWHFMGVMLSAAGSLQWYRDTLAPNVSFDELVKEAESAPAGSEG
                     LLFLPYLSGERTPYPDPLARGSFVGLTLRHTRAHMTRAVLEGVAFGLKDSFTLIQNAG
                     LGKITQV"
BASE COUNT          675 a          681 c          714 g          656 t
ORIGIN      
        1 gaagtggaag acctcgtgga agccattctc ttcgtttggt atcccggcat ggaaggtggt
       61 cgcgctgttg ccgatgtgct cttcggcgat gtttctcccg ctggcaaatt acccatcacc
      121 ttccccaagt cgctcgatca acttcccgcc ttcgacgatt acggcatgac tgggcgcact
      181 tatcggtaca tgaccgccga gccgctttat ccatttgggt ttggcttgag ctacacttgt
      241 tttgaatatt cgaatctaaa actcgataaa acgaatctag cccttggcga ctctctcacc
      301 ctcaacctga ccgtccgcaa cagcggcggc tcagactcag ccgaagtagt tcaatactac
      361 ctgagcgacc tccacgcctc gaccattgtt cccctccatc atctcattgg ttttgagcgt
      421 gtgcatctca aagcaggcga aagcaaaacg ttgaccttca ccatcacacc agacatgatg
      481 tccttctata atgatgatgg caaactcact ctcgaacctg gagagttccg cgtggaagtg
      541 gggagttgct cccccggcaa acgcggacaa gaactaggcg ctccagcacc ggtcacggcg
      601 ttgttcgagg tcaaataaaa acattggagt tgacatagat ggaaaccgtg aatttctgtg
      661 ggcaagaatg tgtcgtattt ggaaataaag ctatacagct attgattacc cgttccgtgg
      721 gtccgcgcat tctctctttg aaattcacgg gcggggaaaa cctgcttgca gaaatgccag
      781 acttcattac agattgcccc ggttctggca tttatcactt ttatgggggg catcgcctat
      841 ggcatgcccc cgaagtgcca agccgcacct acttacccga tgactcgcca gtagatatca
      901 cacagatcga aaacacatta cacatcgttc aagaagtaga ggcaaagacc ggtttacaaa
      961 aatcattcga tattcaaatg cagggggatt gcgcatctct cagcatcaca catcgtttaa
     1021 ccaacaaagg actttggtcg gcaaccaccg ctttatgggc aatcactcaa ttcaagccgg
     1081 gcggacttgg catcttgccg caaacggttg aaaatacagg tgtattgccc aaccgcaggc
     1141 tcaccttctg gccctatacc gatccatctg aaccggctgt gcacctcgga aagaactata
     1201 tactgatcaa cgccaacatg cagggagcgt tcaaagttgg cttctcgaac ccgcgcggtt
     1261 ggcaagccta ttggtggaat ggagttttgt tcgtaaaacg cgccaaatat gaggcagatg
     1321 ccgagtattt tgactatggt agttcaagcg aaagctattg caaccatgag atccttgagc
     1381 ttgagacctt atctcccatc acaaccatag aacccggcgc agaagctact catgtagaga
     1441 catgggaaat gtaccaaggt attgaacgcc cacaaaatga acaggatgtt caagccttgg
     1501 tagaacgcct ggaattggag taaagcatat gaactatttt ctcggaattg acacatccac
     1561 cacatctagc aaggcattat tgatcgactc aaacggcgtg gtgattgcgg tcgctgcggc
     1621 atcgcatacg ttgcaaacac ccaagccgtt atggtcggag caaaacccgc gcgagtggtg
     1681 ggatgcggtt gcagcgagta tcaagtctgt gttggaaaag gcaggcgtga gcggggaaag
     1741 agtcgtcgct gtcgggctga cgggtcaaat gcatggctta atccttttgg atgaaaacgg
     1801 gagtgtgttg cgtcctgcca tcttgtggaa tgaccaacgc acgcaaagtc agtgcgatga
     1861 aattcatcaa gtgatcggca aggaaaaatt tattcaaatc accggcaatg tagcgttgac
     1921 cggttttaca gcgccgaaaa ttttatgggt gaaagagaac gagcccagtg tgtatgcaag
     1981 agcaaaacat gtgttgttgc ccaaagatta cattcgtttt tgtttgacgg gcgaatacgc
     2041 catggacaaa gccgatggcg ctggcaccgt tttgtttgac ttgaaagtac gcgactggtc
     2101 cgatgaagtt ttggcggcat tgggcattcc gcgcgaatgg atgccgcgca cgtttgaagg
     2161 cacagcgttc acaggcaaag tgactgaaga agcagcacgt ctcactggtt tgaaagcagg
     2221 cacacctgtc gccgcaggcg gtggtgacca ggcagccggc gctgtgggtg tgggtgctgt
     2281 agaaccgggc attgttggtc tgacggttgg cactagcggc gtggtctttg caacaacgcc
     2341 ttccgcattg atcgaacctg aaggtcgctt gcatgcgttc tgtcatgctg tgccgggtat
     2401 gtggcatttt atgggtgtga tgctttctgc ggcgggaagt ttacagtggt atcgcgacac
     2461 cctcgcgccc aatgtaagct ttgatgaatt ggtgaaagaa gccgagagtg cgcccgctgg
     2521 ttcggaaggt ttgttgttcc ttccttatct cagcggtgaa cgcacacctt acccagaccc
     2581 actcgcacgc ggttccttcg ttgggctaac gcttcgtcac acgcgcgcac atatgactcg
     2641 tgccgtgtta gaaggcgtgg catttggttt gaaagatagc ttcaccttga tccaaaatgc
     2701 aggcttaggc aagatcacac aagtcc
//
LOCUS       DAOQJE010000502         2723 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_26135_length_2723_cov_9.580960, whole genome shotgun sequence.
ACCESSION   DAOQJE010000502 DAOQJE010000000
VERSION     DAOQJE010000502.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2723)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2723)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2723
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26135_length_2723_cov_9.580960"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..240)
                     /locus_tag="PLT08_12505"
     CDS             complement(<1..240)
                     /locus_tag="PLT08_12505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE05337.1"
                     /translation="MKYTVLLTAPYMLPFVDRFKPVFDSYDIELIIPNVEERMEEEDI
                     LKYAGQFDGTVCGDDRYSAHVIEACLPRLKVISKWG"
     gene            complement(244..1773)
                     /locus_tag="PLT08_12510"
     CDS             complement(244..1773)
                     /locus_tag="PLT08_12510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE05338.1"
                     /translation="MKKFLSRLFNAPWYSIVFGAYPILALLSENKGQVEPSAIFRPLL
                     LSLSFSTIILLIAWLVLRKIHKAAFFSVLFLLLFFTYGHVYMYFEEKFPENTAVQWLW
                     VMWVILFVLSIVWVTRPKLTFETAASSLNGMILVLLIMSITQLDFNTSSGNSHRVGAR
                     NAPIQEDLVLPENPPDVYFFLLDSYGRSDLLQSAYGYDNSDFENALKERGFYIANESQ
                     SNYVRTEISLGSSLNMQYLQELDPAFNPQSIGRKVLWDALKHNAVRYNFDTLGYETIN
                     YASGFAWLEVTDSDQFISPPPISSGMTEFEGLFLRTTWARYAEKWGWIDSDAIMAEGY
                     RDRFNTIFNSMDSISKMPEPKFVYAHVISPHPPFVFDPEGNPTNPADFWNEKRQYTGE
                     MYKKGYLNQLPYLNTRMLEAIDTIMANSKTPPVIIIQGDHGPWLQPKDKRMWNFTAVY
                     MPGHEDTLYPTITPVNLFRLVFNNYFGGKYDILKDVSYFSPVPKLYEFTEIPNPYDEG
                     H"
     gene            complement(1791..>2723)
                     /locus_tag="PLT08_12515"
     CDS             complement(1791..>2723)
                     /locus_tag="PLT08_12515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE05339.1"
                     /translation="ALAPSLGVGILFSVIFERVNTASLRSGLVAFLLGVSVLTHHGNA
                     VRVADETAAVQNFWWQVAWRAPKIQEGVTLIASFPGSPLAEDYFIWGPANFIYYPEKQ
                     TNNPLLIKLPAAVLDGNTVTQITTNGGVETPLRRGNYLERDFGNVLVMVQSSNDSCVR
                     FIDGRSPELNSYDDGRVAMIASYSKLDSVVTEGDVPAVPEEIFGAEPERGWCYTYQQA
                     DLARQRGDWDAIPVLLKDALDKGYYPNDGLEWMPFMQAYAVQGNVEEIKSMLKLVVAD
                     KYLRLQVCNSLTNLMQRETLNGDVSALIEKNICK"
BASE COUNT          797 a          646 c          595 g          685 t
ORIGIN      
        1 tccccatttc gaaatgactt tcaagcgcgg caaacaggct tcgatcacat gtgcgctata
       61 gcggtcatcc ccacacacgg tgccatcaaa ctgcccggcg tatttgagga tgtcttcttc
      121 ctccatgcgt tcttccacat tcggaataat cagctcgata tcgtaggagt cgaagacggg
      181 tttaaaacgg tccacaaaag ggagcatgta gggagccgtg agcagaacgg tgtacttcat
      241 cgatcaatgt ccttcatcgt aagggtttgg aatttcagta aactcgtaga gttttggaac
      301 tggcgaaaaa tagctgacat ctttgagaat atcatatttg ccgccaaaat aattattgaa
      361 gacaagccgg aacaagttga ccggtgtaat ggtgggatac aaagtatctt catgaccggg
      421 catgtatacc gccgtgaaat tccacatacg cttgtcttta ggttgcaacc acggaccgtg
      481 atcgccctga ataataatca ctggcggcgt tttggaattt gccataatcg tatcaatggc
      541 ttcgagcatg cgcgtgttga gatagggcaa ttgattcaag tagccttttt tatacatctc
      601 acctgtgtat tggcgcttct cgttccaaaa atcagcgggg ttggttgggt tgccttcagg
      661 gtcaaacaca aaaggcggat gcggtgaaat cacatgagca tagacaaatt tcggctcagg
      721 catttttgaa atagagtcca tgctgttgaa gatggtattg aagcggtcgc gatagccttc
      781 cgccatgatg gcatctgagt cgatccagcc ccatttttcg gcatagcgcg cccaagttgt
      841 gcgcaagaac aatccttcaa attcggtcat gcctgaagaa atgggcggcg gacttataaa
      901 ctggtctgaa tctgtaacct ctagccaggc aaagccagaa gcgtagttaa tcgtttcata
      961 cccaagggtg tcaaagttat aacgcactgc attgtgcttt agagcatccc ataatacttt
     1021 tctgccaatt gattgcgggt tgaatgctgg gtctaactct tgaaggtatt gcatattgag
     1081 cgaagaaccc aatgaaatct ctgtgcggac gtagttcgat tggctttcgt tggcgatata
     1141 aaacccacgt tctttcagag cattttcaaa gtcgctatta tcatatccat atgcagactg
     1201 gagtaagtct gaacgtccat atgaatctag cagaaaaaaa tatacatcag gtgggttttc
     1261 tggcaaaaca aggtcttctt ggatgggcgc atttcttgcg cctacccgat gcgagtttcc
     1321 tgaagatgta ttgaaatcta attgagtaat ggacataatc aacaaaacca aaatcatgcc
     1381 attcaaagaa gaggcggctg tttcaaaagt caacttgggg cgagtaaccc agacaataga
     1441 aagaacaaaa agaataaccc acatcaccca cagccattga accgctgtat tctcagggaa
     1501 tttctcttca aaatacatgt atacatgccc atatgtaaag aaaagcagaa gaaacaaaac
     1561 agaaaagaac gcagctttgt gtattttacg aagcacaagc catgcaatca aaaggataat
     1621 agtactgaat gacaaggaaa gcaacaaagg tctaaaaata gcggatggct ccacctgccc
     1681 tttgttttca gagagcaacg ccaaaatggg atatgcgcca aaaacaatag aataccaagg
     1741 cgcgttaaac aaccgtgaaa ggaacttttt catcgttaca cctctaaaat ctacttacag
     1801 atattttttt cgatcagtgc agatacatcc ccattaagag tttctctctg cattaggttt
     1861 gtgagcgaat tacaaacttg caaccgcaag tatttatctg ctaccaccag cttcagcatg
     1921 gatttgatct cttccacgtt gccttgcaca gcataggctt gcataaatgg catccattcc
     1981 agaccatcgt tggggtagta gcctttatct aaagcatctt tcaataaaac tggaatcgca
     2041 tcccagtcac ctcgttggcg ggcaagatca gcttgttgat aggtgtagca ccagccacgc
     2101 tcgggttctg cgccaaagat ttcctctggc acagcaggca catcaccttc ggtcactaca
     2161 gagtccaatt ttgaataaga cgcgatcatc gccacgcgac catcatcgta tgaattcagt
     2221 tccggcgatc taccatcaat gaagcgtaca caactgtcat tgcttgattg aaccatcacc
     2281 aacacgttcc caaaatctcg ctccaaataa ttgccgcgcc gtaacggagt ttccactccg
     2341 ccattggtgg tgatctgcgt caccgtattg ccatccaaca ctgcggcagg gagttttatc
     2401 aacaacgggt tgttggtttg cttttcaggg taataaataa aattggctgg tccccaaata
     2461 aaataatctt ctgccaacgg gctgccaggg aaagatgcga tcaacgtcac gccttcttgg
     2521 atcttcggcg cgcgccatgc cacctgccac caaaaattct gcaccgcagc tgtttcatct
     2581 gccacgcgca cagcattgcc atgatgcgtc aacaccgaaa cgcccaacaa aaatgctacc
     2641 aagccagaac gcaaagaagc cgtgttaaca cgctcaaaaa tgaccgagaa caagataccc
     2701 acgcctaaag aaggcgctaa cgc
//
LOCUS       DAOQJE010000503         2715 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_26271_length_2715_cov_5.981955, whole genome shotgun sequence.
ACCESSION   DAOQJE010000503 DAOQJE010000000
VERSION     DAOQJE010000503.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2715)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2715)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2715
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26271_length_2715_cov_5.981955"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..269
                     /locus_tag="PLT08_12520"
     CDS             <1..269
                     /locus_tag="PLT08_12520"
                     /inference="COORDINATES: protein motif:HMM:NF020717.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1939 domain-containing protein"
                     /protein_id="HNE05340.1"
                     /translation="TVLHLSDDLYIMQRGGFGDQPGLIYVLNNRGDDWRGEWVQTKWH
                     RAAFRPVAWWGKNDLSRPEDQLTASDGFGKFFAPARGFAVYTLK"
     gene            385..867
                     /locus_tag="PLT08_12525"
     CDS             385..867
                     /locus_tag="PLT08_12525"
                     /inference="COORDINATES: protein motif:HMM:NF023580.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3592 domain-containing protein"
                     /protein_id="HNE05341.1"
                     /translation="MDTNQAKPMGCFGRGCLFFSLHLIWIILFGVGLYYSFNSFRLVT
                     SGTEVDAIIVDMDADNSDGTTTYSPIYEYTYAGETYRYNSVNSSNIVTYDIGDHTTLL
                     VDPSNPHNARQKSFWELWLMPAIMLPVSLGMGFIMILVQVLAARQGRVANMGNVQINM
                     "
     gene            881..2473
                     /locus_tag="PLT08_12530"
     CDS             881..2473
                     /locus_tag="PLT08_12530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE05342.1"
                     /translation="MSTSKSSSPLLRGLLGLLLLLPACGLCTVDQFWLSLSTLLTSFK
                     NIRISMEGDFVGFKNYIAMFTDRTFPTALGYTTGVIFVHILAAAILPPLLALAVHRFS
                     QRGNRRARLWFTLPLAYFGPALAMFGPAFLRDVWKLTSLHITYLLFDGIVTLAVMSAI
                     GLVVYLAVLRPQQEASSLPTGTLFTTWLVTQLALVAYGLQSLNLLAFLVPGRDGKFML
                     GHMLAQTVRNGYFGLTASVSVFMLVVAAFFGILASVILIARKIQISHRAEEPASFSSN
                     PSIPSWVALALGAVAAFFVTAVPLALSLVKAFGNFASFEFGMFLRISLNTVLPSLLAA
                     LFIQLPVAYLGALGISVVRPFGKSSAWLLLLFSPWLFVTSTPLAFAAFQNLRSADLLD
                     SPLALTPPLLLNVPMLFLLTLFFKGQFDSWQASGKDSSPSLKEALSHWVRPSLPLALF
                     LMVVSGLATAQEVFTPYLTSYSREQQTIALAVAQLAGNFNSGSIAAILALFGIPFFII
                     TFSVLAILQNTYLNKLEITSGT"
     gene            complement(2524..>2715)
                     /locus_tag="PLT08_12535"
     CDS             complement(2524..>2715)
                     /locus_tag="PLT08_12535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE05343.1"
                     /translation="VVEPAKAESLFEGRVLPVITNNERIVSGEEIMPYIKELTRLMYD
                     WQLFQSDACYIRDDGNGVC"
BASE COUNT          545 a          809 c          594 g          767 t
ORIGIN      
        1 ccaccgttct gcatctctcc gatgatctct acatcatgca acgcggcggc ttcggtgacc
       61 agcccggctt gatctacgtc ctcaacaatc gcggcgacga ctggcgcggc gaatgggtgc
      121 agacgaagtg gcaccgcgcc gctttccgcc ctgtagcttg gtggggcaaa aacgaccttt
      181 cccgccccga agaccagctc accgcctcag acggcttcgg caaattcttt gctcccgccc
      241 gcggtttcgc cgtgtacacc ctcaagtaaa aacattcccc cagttgtagg aacactgggg
      301 gaatgttttt ttctatcaaa acccatacac tgataatgtt ccttcgtgtt tacccatctt
      361 attgaataac gttagaggtg caatatggat acaaatcaag caaaacccat gggctgtttt
      421 gggcgcggct gtcttttctt ctctttacat ctgatctgga tcattttatt tggtgtcggt
      481 ctttactata gcttcaacag ctttcgcctc gtcacctccg gcacagaagt ggatgccatc
      541 atcgtcgata tggatgccga taattcggat ggcaccacca cctactcccc gatctatgaa
      601 tacacctatg caggtgaaac ctaccgctat aatagcgtta actcatcgaa catcgtcacc
      661 tacgacattg gcgaccatac caccctgctc gtagacccgt ccaacccgca caatgcccgt
      721 cagaaatcct tctgggagtt atggctgatg cccgccatta tgttgcccgt ctctttgggt
      781 atggggttca tcatgatcct cgtccaagtg ctagcagccc gtcagggtcg ggtcgccaat
      841 atgggaaatg ttcagatcaa catgtaaaac ggaaaggtat atgtccacat ccaaatcctc
      901 ctctcccctt ttacgcgggt tactcggttt gcttttgctc ctgcccgcct gcggactctg
      961 cactgtcgat cagttctggt tgagtctcag caccctcctc acgagtttta agaacattcg
     1021 catcagcatg gaaggcgatt ttgtcggctt caagaattac atcgccatgt tcaccgaccg
     1081 taccttccct actgcattgg gctacaccac cggggtgatc ttcgttcaca ttttggcggc
     1141 tgccatcctg ccccctttgc tcgcacttgc cgttcatcgc ttcagtcagc gcggaaaccg
     1201 ccgtgcgcgc ttatggttta ccttgccgtt ggcatatttc ggtcctgcgc tagctatgtt
     1261 cggtcccgcc ttcctacgtg acgtgtggaa gcttacctct ctccatatca cctatctttt
     1321 attcgatggc atcgtcaccc ttgctgtgat gagtgccatt ggcttggttg tgtatcttgc
     1381 tgttttacgc cctcaacaag aagccagttc tctgcctact ggcaccttgt tcacgacttg
     1441 gctggtcacc caattggcgc tggtcgcata tggtcttcaa tcattgaacc ttctggcatt
     1501 cctcgtcccc ggcagagatg gcaaattcat gctcggtcac atgcttgcgc aaaccgtccg
     1561 caatggatat ttcggtctca ccgcttcggt ttctgtcttt atgctggtgg tcgctgcctt
     1621 ctttggaatt ctcgccagtg tgatcctgat tgcaagaaaa attcaaatta gccatcgtgc
     1681 tgaagagcct gcttcttttt caagcaatcc atctattcca agttgggttg ctcttgcgct
     1741 tggtgctgta gcagcgttct tcgtcactgc ggttccactt gctctgagtt tggtgaaagc
     1801 atttgggaac ttcgccagtt ttgaattcgg catgttcctg cggatctcac tcaataccgt
     1861 cctgccttca ttgttggcag cgctcttcat tcagctccct gtagcctatc ttggtgcctt
     1921 gggcatcagc gttgtgagac ccttcggtaa aagcagcgcc tggctcttgc ttttgttcag
     1981 cccatggctc tttgtcactt ccaccccttt ggcatttgcc gcctttcaaa acctgcgctc
     2041 ggcagacctg ctcgattctc cgctggcact cacaccacct ttgctgttga atgtccccat
     2101 gcttttcctg ctcacccttt tctttaaggg gcagtttgat agctggcagg catctggaaa
     2161 agacagttct cccagcctga aagaagccct ctcacattgg gtgcgtccct ctctaccact
     2221 ggctttgttc ctgatggtgg tctccgggct tgccactgca caggaagtat tcacgcccta
     2281 tctcaccagc tactcacgtg aacaacaaac gatcgcactt gccgtggctc aactggcagg
     2341 gaatttcaac tcaggcagca ttgccgccat ccttgccttg tttggcattc ccttctttat
     2401 catcactttt agtgtgctcg ccattctcca aaacacctac ttgaacaagc tcgaaatcac
     2461 gagcggaact taagcataaa aaagcacccc gttgacctta tatcaacggg gtgctttgct
     2521 tcatcaacaa acgccattcc catcatcgcg gatgtaacac gcatcgcttt ggaacaattg
     2581 ccagtcatac atcagccggg tcaactcttt gatgtagggc ataatctctt ccccactcac
     2641 gatccgctcg ttgtttgtga tcaccggcaa aacacgcccc tcaaaaagag actctgcctt
     2701 ggcaggctcc accac
//
LOCUS       DAOQJE010000504         2711 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_26337_length_2711_cov_6.891190, whole genome shotgun sequence.
ACCESSION   DAOQJE010000504 DAOQJE010000000
VERSION     DAOQJE010000504.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2711)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2711)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2711
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26337_length_2711_cov_6.891190"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..330
                     /locus_tag="PLT08_12540"
     CDS             <1..330
                     /locus_tag="PLT08_12540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE05344.1"
                     /translation="VQGLAKRASTFISDTSYLIGGDFQTTKQNLQDLLVVLKGYDEKL
                     TGWLEQVRMLKESAPKWIDNASLSLTVVLLWFAFSQLGLILHGLSIWYGENPWEVLKK
                     LKKSSVA"
     gene            complement(364..1794)
                     /locus_tag="PLT08_12545"
     CDS             complement(364..1794)
                     /locus_tag="PLT08_12545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE05345.1"
                     /translation="MKNYNRTNPNFIQSSRYNLLMLIIVSDLHLGDGSTADSISPSAF
                     KLFARRLSETARFASIRKDGKYRPVQSMDVILMGDIVDPLHSTRWLDTDPGASNYLRP
                     WSDHTTAAYAQKLHEVTRAILDENEESLQVMRRLAAGEEIRLAPASSKGEPDYESRVR
                     LPLKVRFHYMVGNHDWYFHLKGPAFDAIRNEMIQRMGLSNPLNTPFPFDAEEAPELKE
                     LLERHKVYARHGDIYDRFNYNREAGRDYGTIGDVFTMDVCNRYPVEVSKRYRDRLPAG
                     VVNSLRKIANIRPLLASPLWISGQIRQFAGNRKLENDLKKVWDEISDEFLQLDFVRQA
                     DKAFRFDMVDAMELIVKLSGKASFNTINDVVLWVRKKMWGGKRSFSNYATEEPAFKKG
                     TAQYIVYGHTHSYEIVPLALNLSMPVPESQIYFNSGTWHSYYDLAMQNPKDQLFVPYQ
                     ALTYLTFYAAEEHDGRQFEVWSGSYA"
     gene            1839..>2711
                     /locus_tag="PLT08_12550"
     CDS             1839..>2711
                     /locus_tag="PLT08_12550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE05346.1"
                     /translation="MDTLNEFLSAHHYPLELSNQTLSGSGADFLLARAQESRFMLIGE
                     EHGVGSNIDFAAALFRLINPLGYKNYVTEIGPVSARQVNHLLRESDSQAAFEAFYQKY
                     PFSVPFAWMQEEVELLKAVAQHAHEKDNEPVIGIDQEFIFSPQFHFERLHEMCADPAL
                     KTVLEGWLKAEQQAYVDMQNGVSPDKLSVFMNLPLPTGWQELRSWFQAHHADEALAIL
                     DAVQASHVIYRHYVNEEYYDNNNVRALLMRDYFYKAFQRVDSFNPEARFVIKLGANHV
                     SRGFTSMGIQDIGNF"
BASE COUNT          667 a          589 c          735 g          720 t
ORIGIN      
        1 gtgcaagggc ttgccaaacg cgcttctacc tttattagtg acacatccta tttgatcggc
       61 ggtgattttc aaaccaccaa gcagaatcta caagatttat tggtggtgct aaaaggatat
      121 gacgagaagt tgaccggctg gcttgaacaa gtgcggatgc tgaaagagtc cgcgcctaag
      181 tggatcgata acgcctctct ctccctgaca gttgttctgc tctggtttgc cttctctcag
      241 ctcgggctga ttctgcacgg gctgagcatt tggtatggcg aaaacccgtg ggaggtttta
      301 aagaaattaa agaagagttc tgtagcataa aaagaaacgg tctcatttga gaccgtttct
      361 tttttaggca tacgacccag accaaacctc aaactgccga ccatcatgtt cttcggcggc
      421 gtaaaaagtt agataggtga gggcttggta aggcacaaat agctggtctt ttgggttttg
      481 catggcaagg tcataatacg aatgccaggt tcctgagttg aagtagatct ggctctctgg
      541 caccggcata gacaggttca atgccaatgg cacaatctca taactatggg tatgtccgta
      601 gacaatgtat tgagcagtcc cttttttgaa agcaggttct tccgttgcat agttggaaaa
      661 agaccgtttg ccaccccaca tctttttgcg gacccaaagg accacgtcgt tgatggtgtt
      721 aaaagaagcc ttgcctgata acttgacgat caattccatg gcatcgacca tatcgaagcg
      781 gaaggcttta tcggcttgac gcacaaaatc caattgcagg aattcatctg aaatttcatc
      841 ccagaccttt ttcaggtcat tttccagttt gcgattcccg gcgaactgac ggatttgtcc
      901 gctgatccaa agtggagagg cgagcaatgg gcggatgttg gcgatcttgc gcaggctgtt
      961 gaccacccca gcagggagac ggtctcgata gcgtttggaa acttctacgg ggtagcgatt
     1021 gcaaacgtcc atggtgaaga catcgccaat cgtgccataa tcgcgcccag cttcacggtt
     1081 atagttgaag cggtcgtaaa tatcgccatg gcgggcataa actttgtgcc gttcgagaag
     1141 ttctttcagt tcaggtgctt cttctgcatc gaacgggaag ggtgtgttga gcgggttgct
     1201 cagccccatg cgttgaatca tttcattgcg aatggcatcg aaggcgggac ctttgagatg
     1261 gaaataccaa tcgtgattgc ccaccatata atgaaagcgg actttgagcg gaagccttac
     1321 gcggctttcg taatcgggtt cgcctttgct gctggctggg gcaagccgaa tctcttcgcc
     1381 ggctgcgagc cgacgcatga cttgcagtga ctcttcgttt tcatctaaga tggcgcgggt
     1441 gacctcatgc aatttctgag catacgcggc ggtagtatgg tcgctccacg gtctcagata
     1501 gttggaggct ccggggtcag tatccagcca gcgggtggaa tgaagcgggt cgacgatgtc
     1561 gcccatgaga atcacatcca tgctttgaac agggcgatac ttcccatctt tgcgaatgga
     1621 agcgaaacgg gcggtttcag aaagccgccg cgcgaagagt ttgaatgcac tgggggagat
     1681 ggagtctgcg gtggagccat ccccaagatg taagtcgctg acgatgataa gcataagcag
     1741 attatagcgc gaagattgga tgaaattcgg atttgtccga ttataatttt tcaaaagaaa
     1801 gtgattcgca agtctgaaac cttttagtgg agacgttaat ggataccttg aatgaatttc
     1861 tttctgcaca tcattacccc ttggaacttt ccaatcaaac attgagcggc tctggcgcag
     1921 actttttact ggcgcgtgca caagagtctc gctttatgtt aattggtgaa gagcatgggg
     1981 tggggagcaa tatcgatttt gcggcagcgt tgtttcggtt gattaaccca ttgggatata
     2041 agaattatgt caccgagatc ggaccggtga gtgcgagaca ggtgaatcac ttgctgcgcg
     2101 aatcagattc acaagctgca tttgaggcgt tttatcagaa gtatccgttt tcggtgccgt
     2161 tcgcgtggat gcaagaagaa gtggagttat tgaaagctgt cgcacagcat gcgcatgaaa
     2221 aagataacga gcctgtgatt gggatcgatc aggagtttat tttcagcccg caatttcatt
     2281 ttgagcgttt gcatgagatg tgcgcagacc cagccttgaa aaccgtgctt gagggttggt
     2341 tgaaggcaga gcaacaagcc tatgttgata tgcagaatgg agtttcgccg gataagcttt
     2401 cggtgtttat gaacttgccc ttgcctacag gctggcaaga actacggagt tggtttcaag
     2461 cccatcatgc ggacgaggcg ttagccatcc tggatgcggt gcaagccagc catgttatct
     2521 atcgacatta tgtgaacgaa gagtactatg ataataacaa tgtgcgcgcg cttttaatgc
     2581 gggattattt ttataaggcg tttcaacgcg tggactcgtt caacccagag gcgcgttttg
     2641 tgatcaaact cggcgcgaat catgtttcgc gcgggttcac ctctatgggc attcaagaca
     2701 ttgggaattt t
//
LOCUS       DAOQJE010000505         2710 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_26366_length_2710_cov_3.429379, whole genome shotgun sequence.
ACCESSION   DAOQJE010000505 DAOQJE010000000
VERSION     DAOQJE010000505.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2710)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2710)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2710
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26366_length_2710_cov_3.429379"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..603)
                     /gene="tcmP"
                     /locus_tag="PLT08_12555"
     CDS             complement(<1..603)
                     /gene="tcmP"
                     /locus_tag="PLT08_12555"
                     /inference="COORDINATES: protein motif:HMM:TIGR04474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="three-Cys-motif partner protein TcmP"
                     /protein_id="HNE05347.1"
                     /translation="MPIENGVGYGEYTGVKIEHLSKIMAMHMAITQAVIKKHASYYEP
                     EYRYIDLTAGKGSTPDGSSGSPIIFLDKTSDSKFDIPFRADLIERKPNNIEELKKAVS
                     AHPMGSSQQGNIHFHLGDYESIAPQLFKSKKDFELGLVYVDPSGDLPDFKTLTHIINM
                     RPKMEILLYLSATNIKRQYQQTQKFLADFISETSKEHWLIR"
     gene            720..2603
                     /locus_tag="PLT08_12560"
     CDS             720..2603
                     /locus_tag="PLT08_12560"
                     /inference="COORDINATES: protein motif:HMM:NF015722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HNE05348.1"
                     /translation="MPESNRPLKVFLSYASQDKSLVRELSRRLVGEGWIDTWQDEKNL
                     LPGQDWRVKIEEAVEEADIVIIVLSQNSVSKEGHVQKELRYAREIALEKPEEAIFLIP
                     LRLDECEVPRGLRFYQWVDYFGERKNNGYDALVKSLKLRYEQKLKLEELERARLEKAR
                     LEAEQEAAEELVRQKAAKEKAEYEADEKVAREKAEKAAAEKARLEAEELAKEKNAKEK
                     AEREATEKVAREKAEKEAAEKAQLEAEELARQKAAKEKAEREAAEKARLETEELEKQK
                     AVKEKAKHDSDEKATREKVKPVQKPEIKKLQGNSNVVYWIVGIFVILLVIGLSSLNGT
                     PSTPVPTSTQASLPQSSSSSVSTQKSAPSNTFTKIASTSTPLPTNTPKPWSTEITDDK
                     GAKMALVPAGKFKMGNNSYPDEMPIHESEKPVHEVYLDTYYMDVYEVTNAAYKMCVDN
                     GVCSPPSITNSNARVTYYGNSRFDNYPVIYVDWNMANLYCKWRGSRLPTEAEWEKAAR
                     GIDGRIYPWGNDDVTCALANRDSCNGDTAAVGKYEDGKSPYGMYDMIGNVSEWVSSLY
                     MSYPYDPSDGREETSSSAQRVWRGGSWDYRYDPLPSSTSRRGASPDSAVFSVGFRCAK
                     SAP"
BASE COUNT          830 a          505 c          631 g          744 t
ORIGIN      
        1 tcgtatcagc cagtgctctt tgcttgtttc gctgatgaag tctgcgagaa atttttgagt
       61 ttgttgatat tgtcttttta tgtttgttgc ggataaataa agaagaattt ccattttcgg
      121 gcgcatgttt atgatatggg ttaatgtctt gaagtctggt aagtcgccag atggatcaac
      181 gtaaacaagt cctaattcaa aatccttttt tgatttgaat aattgagggg caatgctttc
      241 atagtcgcca aggtgaaaat gaatattacc ttgttgtgaa gaacccattg gatgagcaga
      301 tacggctttt tttagttctt cgatattgtt aggtttgcgc tctattaaat ctgcccgaaa
      361 tggtatatca aattttgagt cgcttgtttt atcaagaaaa ataattggac tgcctgaact
      421 tccgtctggc gtgcttcctt tgccagccgt taaatcaata tatctatatt caggttcgta
      481 gtatgaggcg tgttttttga ttactgcttg agtaattgcc atgtgcattg ccatgatttt
      541 ggacaaatgc tcaattttta ctcctgtgta ttccccgtat ccaactccat tttctatagg
      601 catatattac ctcctgatag ccgatttgta atttggattt taacacagct taattgattt
      661 tatttagggt tttcggttgt ataatctgaa taatcaattt tggcgggaca aacccaccta
      721 tgcccgaatc caaccgccct ctcaaagtat ttctctccta tgcctcacaa gacaaatctc
      781 ttgtgcggga actttcacgt cgacttgttg gcgagggctg gattgacact tggcaggacg
      841 aaaagaatct attgcctgga caagattggc gggtaaaaat cgaagaggct gtggaagaag
      901 cggatattgt tattattgtt ctttcgcaaa actccgtaag caaagaggga catgttcaaa
      961 aagaattgcg ctatgcgcgc gagatagcgc ttgaaaagcc ggaagaggct attttcctga
     1021 ttccattgcg cttggatgag tgcgaagttc cgcgtggttt gcgcttttat caatgggtgg
     1081 actattttgg ggaaagaaaa aacaatggtt atgatgcttt agtcaaatct ctgaaattgc
     1141 gttatgaaca aaaactgaag ttggaagaat tggaacgtgc tcggcttgag aaagcgcgat
     1201 tggaagcaga gcaagaggct gccgaagaat tagtaaggca aaaagccgca aaagaaaagg
     1261 cggaatacga agcggatgaa aaggttgctc gcgagaaggc agaaaaagcg gctgccgaaa
     1321 aagcgcgttt agaagccgaa gagttagcaa aggaaaaaaa tgcaaaagaa aaagcggagc
     1381 gcgaagccac tgaaaaggtt gcccgcgaga aagcagaaaa agaggctgcc gaaaaagcgc
     1441 agttggaagc agaagaatta gcaagacaaa aagccgcaaa agaaaaggcg gagcgtgaag
     1501 ccgctgaaaa agcacggttg gaaacagaag agttagagaa acaaaaagcc gtaaaagaaa
     1561 aagcaaaaca cgactctgat gagaaagcca ctcgcgagaa agtaaaacct gtacaaaaac
     1621 ctgaaattaa aaaattacaa ggcaattcaa atgtcgttta ttggatagtc gggatttttg
     1681 tgattcttct ggtgattgga ctttcatctc ttaatggaac accgtcaact cctgtgccaa
     1741 catcaactca agcaagtttg ccacaaagtt cttcttctag cgtgtcaaca caaaaatctg
     1801 cgccaagtaa tacctttaca aagatagcca gcacctccac tccacttccc acaaatactc
     1861 caaaaccctg gtcaaccgaa atcactgatg acaaaggcgc aaaaatggcg cttgttccag
     1921 cgggtaaatt caaaatggga aataatagtt accccgatga aatgccgatt cacgaatctg
     1981 aaaagccagt tcatgaagtt tatcttgata catactatat ggatgtctat gaagttacta
     2041 atgcggctta caagatgtgt gtagacaatg gagtttgtag tcctccaagc ataaccaact
     2101 ctaatgctcg ggttacttat tatggaaatt cgagatttga taattaccct gtgatttatg
     2161 tagattggaa tatggcaaac ctttattgta aatggcgcgg ttcaagatta ccaactgaag
     2221 cagagtggga aaaagccgcg cgtggtatag acggtagaat ttacccatgg ggaaatgatg
     2281 atgttacttg tgccctagca aatcgtgaca gttgtaatgg tgataccgct gctgtaggta
     2341 aatatgaaga cgggaaaagt ccatatggta tgtatgacat gataggcaat gtttcggaat
     2401 gggtaagtag tttgtatatg tcatatccat atgaccctag tgatggacgt gaagagacaa
     2461 gttcttcggc ccaacgtgtg tggcgaggtg gctcgtggga ttacagatat gatcccttgc
     2521 ctagttctac aagtcggcgt ggtgctagcc ctgattccgc tgtttttagt gttgggttcc
     2581 gttgtgccaa atctgctccc tagttcaaag cacgtacctg gtaaacatag cccgacattc
     2641 cacacaacat ggcggttttt caccatgtta tactctttca aaacgaagaa tcaaacctaa
     2701 tgacacaagt
//
LOCUS       DAOQJE010000506         2699 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_26566_length_2699_cov_5.397126, whole genome shotgun sequence.
ACCESSION   DAOQJE010000506 DAOQJE010000000
VERSION     DAOQJE010000506.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2699)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2699)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2699
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26566_length_2699_cov_5.397126"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(39..662)
                     /locus_tag="PLT08_12565"
     CDS             complement(39..662)
                     /locus_tag="PLT08_12565"
                     /inference="COORDINATES: protein motif:HMM:NF013209.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HNE05349.1"
                     /translation="MKSTRDKILQTLLKKPKSTINDLADAVGINPISVRHHLTNLQME
                     GLVESQEERHGVGRPRLVYVLTDEGMERFPTRYLRLTTRLIAQMKESIPGPMVSKLFG
                     QIAEDLANEYANDIKGLSMEERLDFVKDMLAQEGFTVEWEKKDGQYQIHEISCPYYQI
                     GVAHPEVCTVDQTLISKMLALPANKVRCILDGGAHCTYVVQPVGKTK"
     gene            complement(734..>2699)
                     /locus_tag="PLT08_12570"
     CDS             complement(734..>2699)
                     /locus_tag="PLT08_12570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE05350.1"
                     /translation="LRLDLTDNDSILNSLSIPLGGSGAANGNFSAGEIYTIDHTSPSV
                     SSIATASLNPSNAATVDFAVTFSEPVSGVDLADFSVSTTNGAVLTGISGSGMNYVLTV
                     ATGTGNDGLRLDFIDNDSVLDLAGNPTIVGFTSGGSYSVDRTAPVVTSITPISQTNET
                     SVDYAVSFSEPVTGVDGSDFFLFTMNGASITNVSGSGNQYLVSISLNPGSDSVRLDVN
                     DNDSILDSVGNPLGGIGAFNGNFIGSTFNVAIDTPIATSIIRANNNPTNAANVNFIVT
                     FSEPVEGVDIGDFILSPNAVITNITNSNPFYIITVNSENINGELKLQLNDDDTIHNAQ
                     GIPLGGNGIGNANFTTGETYLIDRISPQVTSIIRAGGNPAINPTADFIVTFSEPVNGV
                     ETSDFILTSSNVIQSSVVGMQNADPFYWVKVSTGIGSGSIRLDLLDNGNITDKAGNPL
                     LNRNFTGESFNIAKTPVDFSAPTMNSVQGKLTNNPLVSISWSAVRNAQAYEVFVARDS
                     NFSQIVFIQTTTSTSITPPTPLADGGYYVRVRAYNPDLNPGKFSKAINFAVDATPPVA
                     PNLLSPASGATTTKRAWLKWSVVNSVVKYQIQVDNNSDFSSPEFDATTSKATIQTKSL
                     PARTYYWRVRGIDSAGNWSAWSQTFLFTTK"
BASE COUNT          652 a          595 c          685 g          767 t
ORIGIN      
        1 atttcgttaa tttgagaatc gctcataaag tcctcttttt attttgtttt accgacgggt
       61 tgaaccacat acgtacaatg agcaccgcca tctaaaatgc agcgaacttt gtttgcaggc
      121 agtgccagca ttttggagat cagagtttga tctaccgtgc agacttctgg gtgtgccacg
      181 ccgatctgat agtatgggca ggagatttcg tggatctggt attgtccatc tttcttttcc
      241 cattccacag taaaaccttc ctgcgccaac atatccttga cgaagtcaag gcgttcttcc
      301 atacttagac ctttgatatc gttggcatat tcattggcta ggtcttcagc aatctgaccg
      361 aacaacttgc tcaccatggg tccggggatg ctttctttca tctgagcaat gaggcgggtt
      421 gtcagacgca ggtagcgggt agggaagcgt tccatgcctt catcggtcag tacatagacc
      481 aaacgtgggc gtcctacccc gtggcgttcc tcttgcgatt caaccagccc ttccatttgc
      541 aggttggtga ggtgatgccg cacagagatc gggttgatgc cgactgcatc tgccaggtca
      601 ttgatcgtgg attttggctt tttcagtaaa gtttgtaaaa tcttgtctcg cgtagatttc
      661 atgcagggca gtataacttc cctgtaggct cttgtcaata ttctagataa atatcataaa
      721 aattacgaga gggttatttt gtggtgaata ggaaggtttg agaccaggcg ctccaattcc
      781 cagcactgtc tatgccccga actctccaat agtaagttct agctggcagg ctttttgttt
      841 gtattgttgc cttgctggtc gttgcgtcga attcagggct tgaaaaatcg gagttgttgt
      901 ccacttgaat ttgatatttc acaacgctgt ttactacaga ccatttcaac catgcccgtt
      961 tggttgtggt tgcaccagag gctgggctaa gaaggttggg ggcaacgggc ggagttgcat
     1021 ctactgcaaa gttgattgct ttgctgaatt tcccggggtt taggtctggg ttgtaagcgc
     1081 gaactcgtac ataatagccg ccatctgcaa gtggagtagg cggagttatg gatgtagagg
     1141 ttgttgtttg aataaaaaca atttgtgaaa aattactatc gcgtgcaacg aatacttcat
     1201 atgcctgtgc attacgcacc gcagaccatg atatagagac taatgggttg ttggtcaatt
     1261 tgccttgaac ggaattcatg gtgggtgccg aaaaatccac aggggttttg gcaatattga
     1321 aactctcgcc tgtgaaattc ctattcagga gtgggtttcc ggctttgtct gtaatgttgc
     1381 cgttgtctaa taagtcgagc cgaatgcttc ccgaaccgat ccctgtactg accttgaccc
     1441 aataaaatgg gtccgcattt tgcatgccca ccacactcga ttgaatgaca ttggaactgg
     1501 tcaaaataaa gtcacttgtt tccacgccgt tcacgggctc cgaaaatgtg acgatgaaat
     1561 ctgccgttgg gttgatagcg ggattaccac ctgcgcgaat gattgatgtc acttgcggcg
     1621 atatacggtc aatgagataa gtttcacccg ttgtgaaatt tgcattccct atcccgttcc
     1681 cgccgagtgg aatgccttgg gcattatgaa tggtatcgtc atcgttcagt tgaagcttta
     1741 gctcgccatt aatattttct gagttaaccg taataatgta aaacggattc gagttcgtga
     1801 tattggtaat aacagcattg ggtgacaaaa taaaatcgcc tatatccaca ccttcaaccg
     1861 gctcagagaa agtgacgata aagttcacgt ttgcggcatt tgttgggttg ttgttggcgc
     1921 gaatgatcga tgttgcaatc ggcgtgtcaa ttgcaacatt gaacgtgctg ccaatgaagt
     1981 ttccattgaa cgccccgatc cctcctagcg ggttaccgac actatccaga atgctatcgt
     2041 tgtcatttac atctaagcgg acgctgtcgc ttccggggtt gagcgagatg gaaacaagat
     2101 attgattccc ggaaccagag acattcgtga tgcttgctcc gttcatagtg aagaggaaga
     2161 aatcgcttcc atccacgccg gtgactggtt cagaaaaact gacggcgtaa tctacgctgg
     2221 tttcgttcgt ttggctaatc ggtgtgatgg atgtaaccac aggtgcagtc cggtcaacac
     2281 tataagaccc tccacttgtg aagccaacaa tggtggggtt tcctgccaag tctaggacgc
     2341 tatcgttatc aataaagtct aggcgcagac cgtcattccc agtccctgtt gccactgtga
     2401 ggacgtaatt catgccagac ccagaaatgc cggttaatac tgcgccattc gtggtggata
     2461 cactgaaatc cgcgaggtct acgccagaga caggttcaga gaatgtgaca gcaaagtcca
     2521 cagtggcggc attgctgggg ttgaggcttg ctgtggcaat cgaactcaca gatggagatg
     2581 tgtggtcaat ggtatagatt tcgcctgccg agaaatttcc gttcgcagcg ccagagccgc
     2641 ctaagggaat gctaaggctg tttaagatag agtcgttatc ggttaggtcg agccgtagg
//
LOCUS       DAOQJE010000507         2697 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_26612_length_2697_cov_7.015519, whole genome shotgun sequence.
ACCESSION   DAOQJE010000507 DAOQJE010000000
VERSION     DAOQJE010000507.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2697)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2697)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2697
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26612_length_2697_cov_7.015519"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(93..539)
                     /locus_tag="PLT08_12575"
     CDS             complement(93..539)
                     /locus_tag="PLT08_12575"
                     /inference="COORDINATES: protein motif:HMM:NF020977.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HNE05351.1"
                     /translation="MDTKAKLNEAMKESMKSGDELRKRTVRMVLAAIKQVEVDKRADL
                     DEMAVTALIQKEVKNRREALEEAKKANRSDLIADNEAEIKVLEEFLPKAMSADELRAL
                     VQAAITETGAAAPSDMGKVMKIVMPKVAGRAPNDAISSMVKELLNQ"
     gene            complement(585..1217)
                     /locus_tag="PLT08_12580"
     CDS             complement(585..1217)
                     /locus_tag="PLT08_12580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE05352.1"
                     /translation="MSRMISKYHIEMTTHALAPHFSERALEIIVAANVRQDALRNQFG
                     HDEIHYDNNAIEAGDRYINEQRGLVISTLLSPGILGAWVAFGRMLHTAQDFYAHTNYI
                     TLWLDQFEGTPPPPPEIEPLKKELTQSPSFHSGKIYFPLDALYFIKPIRPWVLKLLPK
                     DSHGWMNLDTPDQGPKFDYAIAAATKRTAHEFELLQKLLPPEMFTRFTDR"
     gene            1248..1793
                     /locus_tag="PLT08_12585"
     CDS             1248..1793
                     /locus_tag="PLT08_12585"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: protein motif:HMM:TIGR00636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HNE05353.1"
                     /translation="MTFYTAKGDDGTTGLLGEGRVPKYHVRMEAVGTLDEASAALGLA
                     RAQCEAAQTPRILLTVQRDLYKLMAEVAATPENAEKFRGIDEEQVKWIEYETDELSKV
                     VEMPKEFILPGDTLGGAALSLARAVIRRAERRVVELFDGEVVVNPHLQRYLNRLSSLC
                     FVLELLENQHAGRKTSLAKSE"
     gene            1906..2607
                     /locus_tag="PLT08_12590"
     CDS             1906..2607
                     /locus_tag="PLT08_12590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF554 domain-containing protein"
                     /protein_id="HNE05354.1"
                     /translation="MTGTIINVAAILLGGTVGLLFGSRIPERFKNTIIAGMGLFTVGM
                     GLQMFLKSENQLIVLGAILIGTIFGEWLGIEERLQDFGLALEKRFSSDNESGTGSRFV
                     RGFMVASLLFCIGPMAILGSIQDGLTGDYSLLAVKSTLDGFASIAFASTMGIGVMFSS
                     IIIFFYQGGISLLAERLSTIISDPMMNEMTATGGVLLMGVGISNLLELKKIRVGNFLP
                     ALVVAPLIVWVLGKL"
BASE COUNT          648 a          596 c          726 g          727 t
ORIGIN      
        1 gtattgcaat attcgagtac ggcgtggaac gggggggcgt gtggtcatac gaacgactgc
       61 agggagacaa gcgcctccct gcagcgaggt tcttactggt tgagcagttc ttttaccatc
      121 gagctgatgg catcattcgg ggcgcgcccg gcaactttgg gcatgacgat cttcatgacc
      181 ttgcccatat cactgggggc ggcagcaccc gtttcggtaa tggcggcttg cactaaagct
      241 cgcaattcat cggcagacat ggctttagga aggaattctt ccaacacctt gatttccgct
      301 tcattatcgg caattagatc agagcgattg gctttcttcg cctcttccaa cgcctcgcgt
      361 cggttcttca cctctttctg gatcaaggct gtgaccgcca tctcatccaa atcggcgcgt
      421 ttgtctactt ccacttgttt aatggcagcc aataccatac ggacagtgcg tttacggagt
      481 tcatctccgc tcttcattga ttctttcatt gcttcgttaa gttttgcttt tgtatccatg
      541 ggtttgatta tacctttctt gagtgacgca aaaatattaa gatgttatcg gtctgtgaac
      601 cttgtaaaca tttcaggagg taagagtttc tgcagcaatt caaattcgtg ggcagtgcgt
      661 ttggttgcag cagcaatggc gtagtcaaat ttcggtccct gatcgggggt atcgagattc
      721 atccaaccgt gcgagtcttt gggaaggagc ttcaacaccc acggtcgaat aggtttgata
      781 aagtacaagg catccaaagg gaagtagatt ttcccagagt gaaagcttgg gctttgggtc
      841 agctctttct ttagcggttc aatttcgggc ggaggtggag gcgtgccttc gaattggtca
      901 agccacaagg tgatgtaatt ggtgtgggcg tagaaatcct gtgctgtgtg aagcatccgc
      961 ccgaaggcaa cccatgcgcc gagaatgccc ggcgaaagca aggtagaaat aaccaagcca
     1021 cgctgttcat tgatatagcg atctcccgct tcaatggcgt tgttgtcata atgaatttca
     1081 tcatgcccaa attggttgcg gagcgcatcc tgccgtacgt tggcggcaac gataatctcg
     1141 agggcacgtt cgctgaaatg tggagcaagc gcatgggtgg tcatttcgat atgatatttg
     1201 gaaatcatgc gcgacattat aatcacttat ccgctataat gccccgcatg acattttata
     1261 cagcaaaagg tgacgacggc acgaccgggc ttctgggtga aggacgagtg ccgaaatatc
     1321 atgtacggat ggaagcggtt ggcacgcttg atgaagcctc tgccgccctg gggctggcac
     1381 gcgcgcaatg tgaagcggct caaactcccc gaattctgct cactgtccaa cgagatttat
     1441 acaagctgat ggcagaggtt gccgccaccc cagagaatgc cgagaaattt cgcggaatcg
     1501 acgaagaaca agtgaagtgg attgagtatg aaacggacga actgagcaaa gtggtggaaa
     1561 tgcccaaaga gttcatcctg ccaggtgata cgctgggggg tgctgcgctg tcactggcac
     1621 gagcggttat tcgccgggca gagcgacgtg tggtggaact cttcgatggc gaagtagttg
     1681 tcaacccgca tttgcagaga tatctcaacc gtttgtcttc attgtgcttt gtactggaac
     1741 tacttgagaa tcaacatgct ggcagaaaga cttctttggc aaagtctgaa taattccaaa
     1801 gaactttcat aaaaacagaa tttcactctg agcggcagcg aagagtctct agctatgggt
     1861 agagatgctt cgctgtcgct tagcatgaca atagaaaaga gaatcatgac tggaacgatc
     1921 atcaatgttg ccgccatttt gctcggggga acggtaggac tattattcgg ttcgcgcatc
     1981 ccagaacgtt ttaagaacac catcatcgca gggatggggc tgttcaccgt aggtatgggt
     2041 ttgcaaatgt tcctcaaaag cgaaaatcaa ttgatcgtat taggcgccat tctgattggc
     2101 accatctttg gggaatggct tggaattgaa gaacgtttgc aagattttgg cttggcatta
     2161 gaaaagcgct tctctagcga taatgaaagc ggcacaggct ctcgtttcgt gcgtggcttc
     2221 atggtggcat ccctgctatt ttgcataggt ccaatggcaa ttcttggctc cattcaagat
     2281 gggttgacgg gtgactatag cctgctggcg gttaaatcca cgttggatgg gtttgcctct
     2341 attgcctttg cttccacgat gggaatcggt gtgatgtttt cttcgatcat tatcttcttt
     2401 tatcaaggtg gcatcagttt gctggcagag agactttcca ctattatcag tgaccccatg
     2461 atgaatgaga tgaccgccac aggtggggta ttgttgatgg gggtgggaat cagtaatcta
     2521 ctggaactca aaaagattcg ggttgggaat tttctaccgg cgctggtcgt tgctccactc
     2581 attgtgtggg tgttaggaaa gctataaaca aaaaacatcc ccaaatgggg atgttttttg
     2641 actggtgact ccggggggat ttgaaccctc aaccaattga ttaagagtca actgctc
//
LOCUS       DAOQJE010000508         2696 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_26627_length_2696_cov_9.032942, whole genome shotgun sequence.
ACCESSION   DAOQJE010000508 DAOQJE010000000
VERSION     DAOQJE010000508.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2696)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2696)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2696
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26627_length_2696_cov_9.032942"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..552)
                     /locus_tag="PLT08_12595"
     CDS             complement(<1..552)
                     /locus_tag="PLT08_12595"
                     /inference="COORDINATES: protein motif:HMM:NF015451.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HNE05355.1"
                     /translation="MTKTVAIIGGGPAGLMAAEVLSQNADLQIDVYDAMPSLGRKFLM
                     AGKSGLNLTHSEPFEKFVSRYSRKEVEAWLHAFTPDNLRTWAKELGVDTFVGTSGRVF
                     PVGMKASPLLRAWIQRLSASGVTFHLRHKLSGLPADTSLHFETPNGNVNAKADAVILA
                     LGGGSWRKLGSDGAWAEWLSQAGV"
     gene            complement(608..775)
                     /locus_tag="PLT08_12600"
     CDS             complement(608..775)
                     /locus_tag="PLT08_12600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE05356.1"
                     /translation="MILNRTTRNILIAISAGVATGIFLNMLSMSLPSLIVGVVVATGV
                     TFALVHSRKDS"
     gene            complement(781..1842)
                     /locus_tag="PLT08_12605"
     CDS             complement(781..1842)
                     /locus_tag="PLT08_12605"
                     /inference="COORDINATES: protein motif:HMM:NF024183.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HNE05357.1"
                     /translation="MQRRSKGVVQLEWVCPNCDTRNPGPVKNCQNCGAAQPENVQFQR
                     AADEKLITDEKVVQAAKAGADIHCGFCGTRNPATAETCSQCGADLKEGKARQAGQVMQ
                     AAPSTPKVVNCSNCGSENSGSAKTCTNCGAPIKLPQAKAPAPIAASAQAGKVAAQANK
                     KPVNWLVAGGIGAVLVMCCIAVLMLFVFPSRSVTGTVSGVSWQTSVPVQEVQPVNYSN
                     ERGNPPSDAYNVSCRTDSQEVCEDKTIDKGNGYAEVVTECHTVKDQYCSYTVDEWTTI
                     QTYDLNGTDLNPEYSSPNISSDQRLGNSSTQLQVYFSTSDGELTYSPNSVTEFQQYQP
                     GSQWTLKLNAVGSVLSVER"
     gene            complement(1966..>2696)
                     /locus_tag="PLT08_12610"
     CDS             complement(1966..>2696)
                     /locus_tag="PLT08_12610"
                     /inference="COORDINATES: protein motif:HMM:NF019982.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HNE05358.1"
                     /translation="VPRLDGSVYTGIDCAFWPSSPKEEVTREPLKLDGVPVLVLNATL
                     DPATPFEEGKFVAENLADGYHIYVEGGVHSIFGYGYACPDQYVTDLLVDGKVPDQREI
                     QCTDWENPVYANYSPNLPASANDFADALDMMIYLDENFYYLPEVYLNDWSEEKSIGCT
                     YGGTYTFAPSEKGEDHTYENCQMMPGVTVNGTGTFDYNAGVFSMAVNISGDKTGELNY
                     SYDFNTGTTSVTGTFEGEPVDLSQ"
BASE COUNT          623 a          680 c          690 g          703 t
ORIGIN      
        1 aaccccggct tgactcaacc attcggccca tgccccgtcc gagccgagtt tgcgccaact
       61 accgccaccc aaagcaagga tgacagcatc agctttcgca ttgacattgc cattgggtgt
      121 ttcaaaatgt agagacgtat cggctggcaa cccgctcaat ttgtggcgca ggtgaaaggt
      181 cacgccagag gcgctgagtc tttgaatcca cgcccgcagt agtgggctgg ctttcatccc
      241 aaccggaaag acgcgccctg aagttcccac gaacgtatct acaccgagtt cttttgccca
      301 ggtacgcaaa ttatcgggcg tgaaggcatg cagccaagct tcgacttctt tgcggctgta
      361 gcgcgaaacg aatttttcaa aaggttcaga gtgggtaagg ttgagcccgc ttttgcctgc
      421 cattaaaaat ttgcgcccca gcgaaggcat ggcatcatat acatctattt gaaggtcagc
      481 gttttgtgat aagacctctg ctgccattaa gccagccggt ccgccgccga tgattgctac
      541 ggttttcgtc atggggttca ttataaagag aaactcccga aagggagttt ctctttatat
      601 ataacgatta actatctttg cgagaatgca cgagcgcaaa tgtcacaccc gtggcaacga
      661 caacgcccac gataagcgaa ggcagcgaca tggatagcat gttaaggaag atgccggttg
      721 caaccccggc tgaaattgcg atcaagatat ttcgggtcgt tctattgaga atcatagggc
      781 ttatcgttct acgctgagca cactgcccac ggcatttaac ttcaacgtcc actgactgcc
      841 gggttgatat tgttgaaact cagtgaccga gttgggcgaa taggtcaact cgccatcgct
      901 ggtgctgaag tagacctgta actgggtaga gctattcccg aggcgttgat cgctggaaat
      961 gttggggctg gaatattcag ggttcaggtc tgttccattt aggtcatagg tctgaatggt
     1021 tgtccactcg tcgacggtgt aactgcaata ttggtctttt acggtgtgac attctgtcac
     1081 tacttcagcg tagccattgc ctttgtcgat ggtcttgtct tcgcagactt cttgactatc
     1141 ggttcggcag gaaacgttat acgcatcaga ggggggattt ccgcgttcgt ttgaataatt
     1201 gaccggttga acttcttgca ctggaaccga tgtttgccaa gagacaccag agactgtgcc
     1261 cgtgacagaa cgagagggga acacgaacaa catcaaaaca gcgatacagc acatcaccaa
     1321 aacggcgcca atgccgcctg ccaccaacca gttgactggt tttttatttg cctgcgcagc
     1381 aaccttgccc gcttgtgcag atgccgcgat gggggcgggc gctttggctt gtggcaattt
     1441 gatgggcgca ccacagttcg tacaggtttt ggcactgcct gagttttcgc ttccgcaatt
     1501 cgaacagttg acaactttcg gcgtggaagg agccgcttgc atcacttgcc cagcctggcg
     1561 ggctttgcct tccttcaaat ccgcgccgca ttgcgaacaa gtctctgctg tggcggggtt
     1621 gcgcgtccca cagaatccgc agtggatgtc tgcacccgct ttggcagctt gcaccacctt
     1681 ttcatcggtg atcaattttt catcagcggc tcgctggaat tggacatttt caggttgggc
     1741 cgcgccgcag ttctggcagt tcttgacggg tcccgggttg cgcgtgtcgc agtttgggca
     1801 tacccattcc aactgcacaa cgcctttact tcttctttgc atggtctact ccttatcaat
     1861 cattgcgtat gtgccaagtg tacaacaatc tgtgcatgga ttgaatggca tcattaaaaa
     1921 caccttcgag ggtatcgaag gtgtttaggc atcgcaatta atgttctact gcgataggtc
     1981 taccggttct ccttcgaatg tgccggtcac agatgtggtt cctgtattga aatcatacga
     2041 gtaattgagc tctccggttt tatctccgct gatgtttact gccatgctga aaacgccggc
     2101 gttgtaatca aaggtgcctg tgccgttgac ggtaacgccc ggcatcattt ggcaattctc
     2161 ataggtatgg tcttcgcctt tttcactggg tgcaaaggtg tacgtgccac cataagtaca
     2221 gccaatagat ttttcttcag accaatcgtt gagatacact tcgggtaaat aatagaagtt
     2281 ctcatcgaga tagatcatca tatccaatgc gtctgcaaag tcatttgcac ttgcgggtaa
     2341 gttgggagag tagtttgcgt acacggggtt ctcccagtct gtacattgga tctcgcgttg
     2401 gtctggaact ttcccgtcta ctaaaaggtc ggtcacatat tggtcgggac aggcataacc
     2461 atacccgaag atggagtgaa cgccgccttc cacataaatg tgatagccat ctgctaagtt
     2521 ctcagccaca aacttacctt cttcgaacgg agtagcaggg tcaagggttg cattcaaaac
     2581 caaaacgggc acgccatcta acttcaacgg ctcacgtgta acttcttcct tgggcgaact
     2641 aggccagaag gcacagtcga tgccggtgta gaccgagccg tccaaacgtg gaacgg
//
LOCUS       DAOQJE010000509         2696 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_26629_length_2696_cov_5.318819, whole genome shotgun sequence.
ACCESSION   DAOQJE010000509 DAOQJE010000000
VERSION     DAOQJE010000509.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2696)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2696)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2696
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26629_length_2696_cov_5.318819"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..212)
                     /locus_tag="PLT08_12615"
     CDS             complement(<1..212)
                     /locus_tag="PLT08_12615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE05359.1"
                     /translation="MQKKLFVLLSLLVMASMLLAACGTPATEAPAATEASSTEAAGTE
                     APAATEAPANAEVTLKVYLLDYTPDT"
     gene            complement(295..1143)
                     /locus_tag="PLT08_12620"
     CDS             complement(295..1143)
                     /locus_tag="PLT08_12620"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019213 - deacetylase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HNE05360.1"
                     /translation="MKFHLNTAEIYIPDNEPEENALSRTTHLCLAAHQDDIEIMAAQP
                     IIECFQQKDKWFTGVVVTDGRGSPRNSIYENYSDDEMRLVRFKEQRKAAIVGEFAAQI
                     MLDIPSKIIKDASRPEPVDDILAILRATKPKVVYTHNLADKHDTHVGVTLRVIEALRK
                     LSPAERPERVVGCEVWRALDWMVDSDKVTMDLSRHENLQFALLGVFDSQIVGGKRYDL
                     ASMGRRRANATYFESHGVDNSEGLSYGMDMTVLMNDVSIDPAKFIGDFMQRFKDDVNQ
                     RIIRMS"
     gene            complement(1153..2538)
                     /locus_tag="PLT08_12625"
     CDS             complement(1153..2538)
                     /locus_tag="PLT08_12625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HNE05361.1"
                     /translation="MQLIEPKVTPPLDESFRPAVLADKNFRRNAQPVGVRAVIGLERG
                     AGEMSRFETIVFPEGHPDFESNYQYIERIVKFLLWQRGGHTVYVGAPKQIGEHIRKVY
                     AADGDGAQKFDFHFMGEQVYEKEFSVVVCNADEVPASRETGKLLGRNLAGCRIGFDLG
                     ASDRKVSAVVDGEAIYSEEVIWEPRKNTDPSYHYQEVMAALKTAASKMPRVDAIGGSS
                     AGIYVDNRPMVASLFRGIPAENFGDIKNMFLRIRDEMGVPLEVINDGDVTALAGSMSI
                     EDNGILGIALGSSEAAGYVNMEGHIMGWLNELAFAPIDYSPSAPVEEWSGDKGCGASY
                     FSQQCVFRLAPKAGIEVPANVTDAEKLKFVQKKLEVGEDGATKIWQSMGIYLGYGIAH
                     YAEFYDIKHVLILGRCTSGRGGDLLIEGAKKVFETEFPELLEKIELHLPDEKIRRVGQ
                     SVAAASLPVVE"
BASE COUNT          696 a          768 c          565 g          667 t
ORIGIN      
        1 atggtgtcag gggtataatc aagcaggtaa accttcaagg ttacctctgc gtttgcaggg
       61 gcttccgtag ccgcgggagc ctctgtaccg gcagcttcag tggatgaggc ttcggtagca
      121 gcaggagctt cggtggcggg ggtaccgcag gcagccaaga gcatgctagc catcaccaaa
      181 agcgataaca aaacgaacag tttcttttgc atttttcttc tccttatgtt tttaatttct
      241 ttttcctgag caagcgtctt tcttcgctaa cgcttctttc cgaataaaac aggatcaact
      301 catcctgata attcgctgat tcacatcatc cttaaatctt tgcataaaat caccaataaa
      361 ttttgccggg tcgatactta catcattcat cagaaccgtc atatccatgc cataagacaa
      421 gccttcagaa ttatctacac catgcgattc aaaatatgta gcgtttgcac ggcgacgtcc
      481 catcgatgct aggtcgtagc gtttaccgcc cacgatctgc gagtcgaaca cacctaataa
      541 agcgaactga agattttcat gacgcgataa gtccatggtg accttatcgg agtcgaccat
      601 ccagtccaac gcacgccaga cttcacaccc cacgactctt tcgggacgtt cagccgggct
      661 cagctttctg agagcttcaa tcactctgag agtgactccc acatgcgtat cgtgcttatc
      721 tgccaagttg tgggtataga ccaccttcgg cttagtggca cgcaaaattg ctaaaatatc
      781 gtctacaggt tccgggcgag atgcatcttt aatgatcttg ctcggaatat ccaacataat
      841 ctgtgccgcg aactcaccaa caatcgccgc cttacgctgt tctttgaaac ggacaaggcg
      901 catctcatca tcagaataat tctcgtaaat actattacgt ggagagccac gtccatccgt
      961 tacaaccaca ccagtgaacc acttatcctt ttgctgaaag cattcgatga tcggctgcgc
     1021 cgccatgatc tcaatgtcat cttgatgcgc ggcaagacat aagtgtgtag tgcgcgaaag
     1081 cgcattctct tccggctcat tatcagggat ataaatttct gctgtattaa gatgaaactt
     1141 cattgttact tcctattcaa ctaccggcaa actcgccgcc gccaccgact gccccacccg
     1201 gcgaatcttc tcatccggca gatgcagttc aatcttttcc aataattcag ggaattccgt
     1261 ttcaaagact ttcttcgcac cttcaattaa caagtcgccg ccacgcccag aggtgcagcg
     1321 tcccaagatc aaaacgtgtt tgatatcgta aaactcagca tagtgagcaa tgccataccc
     1381 gagataaatg cccatgcttt gccaaatttt ggtcgcgccg tcctcgccaa cttccaattt
     1441 cttctgtaca aacttgagct tctcagcatc cgtcacattg gcagggactt caattcccgc
     1501 tttcggtgcc aaacgaaaca cacattgctg tgagaaatac gaagcgccac agcctttgtc
     1561 accagaccat tcttccacag gtgcggatgg gctgtagtca atgggcgcga acgccaattc
     1621 attcagccag cccataatgt gaccttccat gttcacatat ccagccgctt cgctcgaccc
     1681 aagcgcaatg cctaaaatgc cgttatcttc aatcgacatc gagccagcca gcgcagtcac
     1741 atcgccatcg ttgatgactt ccaacggcac gcccatctca tcacgaatgc gcaggaacat
     1801 atttttgata tcgccaaaat tttcagccgg aataccgcga aataatgaag ccaccatcgg
     1861 gcgattgtcc acgtaaatgc ctgccgagct gccgccgatc gcatccacgc gcggcatctt
     1921 gctcgcagcg gttttcaagg cagccatcac ttcttgataa tgataagaag gatccgtgtt
     1981 cttgcgcggc tcccaaatca cttcttcgct gtaaatcgct tcgccatcca ccacggcgct
     2041 cactttgcgg tcagacgcac caaggtcaaa cccaatgcgg cacccagcca aattacgccc
     2101 aagcaatttc ccggtctcgc gcgaagcggg cacttcatcc gcattacaaa cgaccacaga
     2161 aaattctttt tcatatactt gctcccccat aaagtgaaag tcaaactttt gcgcaccatc
     2221 accatccgcc gcatacacct tgcgaatgtg ctcaccaatt tgcttcggcg cgcccacata
     2281 cacggtatgc cctccgcgct gccaaagtaa aaacttcacg atgcgttcga tgtattgata
     2341 attggattca aaatcgggat gaccttcggg aaagacgatg gtctcgaagc gcgacatttc
     2401 gcccgcgcct ctttccaagc cgatcacggc tcgcacacca acaggctggg catttcgccg
     2461 aaaatttttg tctgccaaca ctgccggacg aaaactttca tccaatggcg gcgtgacttt
     2521 gggttcgatc aattgcatgg actagttttc ctattgaaac aaaagtgaat aatcatcgag
     2581 cagcaagaaa gcggaagcac cgagaatgca agcgcgataa tcgagcgcac cgagttcaag
     2641 tttcgtattt tcagagagcc gcgccaacgc cgcattgcgc atggagttat tgacct
//
LOCUS       DAOQJE010000510         2692 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_26705_length_2692_cov_4.744786, whole genome shotgun sequence.
ACCESSION   DAOQJE010000510 DAOQJE010000000
VERSION     DAOQJE010000510.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2692)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2692)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2692
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26705_length_2692_cov_4.744786"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..722
                     /locus_tag="PLT08_12630"
     CDS             <1..722
                     /locus_tag="PLT08_12630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE05362.1"
                     /translation="GDLAEIAISEFINQYMYDFKSIHVGKDLYRAKSKEEDITVENEI
                     TNEKFPISLKAYGDGPLQLSTDKNFQMYPLLEGIGERINDKAQIAKIFENEAFSCFSE
                     INVLPLIYDEKKQRCNILVFDAERARNETAYIKKETEGSGRKHPAYRFFDKNDCYICE
                     VRYGNAAANALQRGLWTNTKNATPFFESVTNGWVDYSHNLVLVKLFSHALVSSAKGHE
                     TALEEIKSDIAKLKQANGINA"
     gene            715..1857
                     /locus_tag="PLT08_12635"
     CDS             715..1857
                     /locus_tag="PLT08_12635"
                     /inference="COORDINATES: protein motif:HMM:NF014177.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009007 - site-specific
                     DNA-methyltransferase (adenine-specific) activity
                     [Evidence IEA];
                     GO_process: GO:0032775 - DNA methylation on adenine
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA adenine methylase"
                     /protein_id="HNE05363.1"
                     /translation="MRNYSLSLFGSAILDTPDTQGVKYAGSKLKLIPHILSLFDELEV
                     ETVFDGFSGTTRVSQALAKSGFKVTSNDISEWSYVFGLCYLKNKKKISEYKELIEHLN
                     SIKGYDGWFTENYGGRDFSGSAVQPDGTKKPWQIHNTRKLDGIRDEIDALCLPKVDRA
                     VALTSLILAMDEVDSTLGHFTSYLKDWSSRSYKEMRLKVPKVFENTEENTVLKGDIFE
                     SMKNVAVDFAYFDPPYGSNNEKMPPSRVRYASYYHVWATICKNDKPNVFGAALRREDT
                     SDRVAATVFEEFRKDNDGHFIAVKAIEKLIETANAKYVALSYSSGGKATAAELNDILN
                     RHGKLIKTIELDYKKNVMADMKWTNEWLRDAEEPNREFIFLIDKQA"
     gene            1930..2421
                     /locus_tag="PLT08_12640"
     CDS             1930..2421
                     /locus_tag="PLT08_12640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE05364.1"
                     /translation="MLSHIPIWVFFLFGGLFVLGLWLGRPRAVSPRLQIIVAVGFLGY
                     SLWGVISAFGIHPAAMLLWAAGVLASVTVLRRSFGPRGLARSEVPSRVQVPGSWAPLA
                     LIMGVFLGRFLIGFAQGARLPLGTHALFGPAVSFLLGSLSGGFAARALAIKSFAARSH
                     SEV"
     gene            2493..>2692
                     /locus_tag="PLT08_12645"
     CDS             2493..>2692
                     /locus_tag="PLT08_12645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE05365.1"
                     /translation="MRIPPGFNTVAPYLFSDDAPRLVQFLVEGLGGVEVLRHLDGDRI
                     ANAQVRLGTSTVMVSQASTAFPA"
BASE COUNT          714 a          636 c          672 g          670 t
ORIGIN      
        1 atggcgatct tgcggaaatt gcaatttcag agtttatcaa ccaatacatg tatgacttca
       61 agtctattca cgttgggaaa gacctttacc gggccaagtc aaaggaggaa gacataacgg
      121 tcgagaatga gataacgaac gaaaaattcc ccataagcct taaagcctat ggtgacggcc
      181 cactccaatt gtccacagac aagaattttc agatgtaccc tttgctagag ggaattgggg
      241 agcgcattaa cgataaagcg cagattgcca agatatttga aaatgaggca ttttcttgct
      301 ttagcgagat taacgttctt cctctgattt acgacgaaaa aaagcagagg tgtaacattt
      361 tagttttcga cgcagagcgt gcaaggaacg aaactgcata cataaagaaa gaaaccgagg
      421 gctctggtcg gaagcatccc gcatatagat tcttcgataa gaatgactgc tacatctgtg
      481 aagtgcgata tgggaacgct gcagctaacg ccctgcaacg tgggctttgg acaaacacaa
      541 aaaatgccac tccatttttt gaaagcgtaa caaatgggtg ggttgactat tcacataact
      601 tggttttggt taagctgttt tcacatgcac tggtgtccag tgccaaaggg cacgaaactg
      661 ctcttgaaga aatcaagagc gatatagcca agctaaagca ggcgaacgga atcaatgcgt
      721 aactactcct tgtcattatt tggcagcgca atacttgata ccccagatac ccaaggggtt
      781 aagtatgcgg ggtcaaagtt gaagctcatt ccgcacatat tgtcattatt tgatgagcta
      841 gaagtcgaaa ccgtattcga tgggttctct ggaactacgc gcgtctcgca ggcactggca
      901 aagtccggat tcaaagtcac aagcaatgac atctctgagt ggtcgtacgt attcggttta
      961 tgctatctaa aaaataagaa aaaaatatcg gaatataaag aattaatcga gcacttgaac
     1021 tccatcaaag gttacgatgg ttggtttact gaaaattatg gaggacgtga tttttcaggt
     1081 agcgcggtac agccagatgg caccaagaaa ccgtggcaaa tacacaacac cagaaagctt
     1141 gatggaatac gagatgaaat cgacgcgctg tgtttaccga aagtcgatag agcagttgca
     1201 ttaacaagtt tgattcttgc aatggatgag gttgatagca cgctcgggca ttttacgtct
     1261 tacctaaagg attggtcatc acgctcctat aaagaaatgc ggcttaaggt gccgaaggtg
     1321 tttgagaata ccgaagaaaa taccgtgctt aaaggcgata tatttgagtc aatgaaaaat
     1381 gttgcggtcg actttgcgta ttttgatcca ccatacggat caaataatga aaaaatgcct
     1441 ccatctaggg tgcgctatgc ttcctactat catgtttggg caaccatttg caaaaatgat
     1501 aagccgaacg tttttggggc ggcacttcgt agggaagaca cttctgatcg agtggcggca
     1561 acagtttttg aagaattccg caaagataat gatggtcatt ttatcgccgt aaaagcgatt
     1621 gaaaaactta ttgaaactgc caatgcaaaa tacgtagctc tttcttatag ctctggcggt
     1681 aaagccactg ctgcggaact caatgacata ttaaatcgtc acggaaagct aataaaaact
     1741 atagagcttg attacaaaaa aaatgtgatg gcagatatga agtggacaaa cgaatggttg
     1801 cgagatgcag aagagccaaa tcgtgagttc attttcttaa tcgacaaaca ggcataacaa
     1861 gtcgttccag cggacggctt cgccgccgct gaactccaac gttagacctc gcaaacaccg
     1921 gagccgccca tgctgtccca cattcctatc tgggtcttct tcctctttgg cggcctgttc
     1981 gtcctcgggc tatggcttgg gcgacctcga gcggtctcgc cgcgacttca gatcatcgtt
     2041 gccgttggat tcctcggcta ttcactttgg ggagtcatca gcgccttcgg gatccacccc
     2101 gccgccatgc tgttgtgggc ggccggagtc ttggccagtg tcaccgtcct gcgcaggtcc
     2161 ttcggtcccc gagggctggc ccgaagcgaa gttccctcca gggttcaggt tccaggcagc
     2221 tgggcaccct tggcacttat catgggcgtg ttcctcggtc gcttcctcat cggcttcgcc
     2281 cagggtgcgc gtcttcccct tggtacacat gctctcttcg ggcccgcagt gtcgtttctt
     2341 cttggctcgc tatccggagg ctttgcggcg cgcgccctcg cgatcaagag ctttgcagca
     2401 aggtcgcaca gcgaggtcta accattcggt caagccgacg tgcctacggc acgcggctta
     2461 cctcaaacgt taggtcgcac atcggaggca ccgtgcgcat tccacctggg ttcaatacag
     2521 ttgctccgta cctcttcagc gatgatgcgc cgcgtctcgt gcagtttctg gtcgaaggtc
     2581 tcggtggagt tgaagtcctc cgccacttgg atggcgatcg cattgccaat gctcaagtgc
     2641 gcctcgggac ctcgacggtc atggtgagcc aggcttcgac agccttcccc gc
//
LOCUS       DAOQJE010000511         2692 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_26710_length_2692_cov_3.528631, whole genome shotgun sequence.
ACCESSION   DAOQJE010000511 DAOQJE010000000
VERSION     DAOQJE010000511.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2692)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2692)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2692
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26710_length_2692_cov_3.528631"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            27..1481
                     /locus_tag="PLT08_12650"
     CDS             27..1481
                     /locus_tag="PLT08_12650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CpaF family protein"
                     /protein_id="HNE05366.1"
                     /translation="MSLLRRIEQGQSSNNQQPSSTSNQGASKQAESEASSNGNSDSSR
                     LSSLQARRVSAPITSPQAGSYFDLKTRVQNKLLAEIDPSMDVTRTDEVRRTIQNLFEQ
                     ILTEENIVLSRPERARLFEQISAEILGFGPLQSLLEDDTITEIMVNGPKNVYIERKGK
                     VHRVPITFESNDHVMRIIDRIVAPLGRRIDESSPYVDARLPDGSRVNAVIPPISLVGP
                     VLTIRKFSKNPITVDQMIQFGSVSAEAVQFLKACVESRLNIIISGGTGSGKTTLLNVL
                     SSFIPSDERILTIENAAELQLRQEHVVTLESRPANIEGRGEITIRDLVINALRMRPER
                     IIVGECRGGETLDMLQAMNTGHDGSMTTAHANTPRDALSRIETMCLMAGMDLPIRAIR
                     EQVASAVDLICQQERMRDGTRKVTTITEVSGMEGDVITMTDIFVFEQTGTENGRIIGR
                     LRPTGLRPKFMDKIEAAGIHLPPSIFGIGERRRY"
     gene            1520..2473
                     /locus_tag="PLT08_12655"
     CDS             1520..2473
                     /locus_tag="PLT08_12655"
                     /inference="COORDINATES: protein motif:HMM:NF012694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HNE05367.1"
                     /translation="MTWVIIFGGIIIIVLLIVGVVVSSNSERSALEERLSQYLDADKT
                     DVDKEAQKYALTNWVSKRVEKTSFGDRIARQLARADLKFKVAEYFALIFISVFLFSLV
                     AWFLGNRHPVSALIGLVIGAIAPGQYVRAQQKQRLRRFNDQLPDMLNLMVNGLRAGYS
                     TMQAMEAISKELPAPINDEFKRVVQEMQIGIPMETALDNLLRRIPSEDLDFVVTAINV
                     QREVGGNLSEILDNISFTIRERIRIKGEVRVLTAQVRTSGTVLSLIPFGLTIILWFLN
                     PDYLLSVTKGGPICTAVIICVVLGLIISSYFIMMKIADIEV"
     gene            2475..>2692
                     /locus_tag="PLT08_12660"
     CDS             2475..>2692
                     /locus_tag="PLT08_12660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE05368.1"
                     /translation="MGVAIAIVVGIALIGGIVFVVIIGARYARDAQGEEADPVLARLA
                     EATQRGENVSLEDIELQQPFMERVVIPVV"
BASE COUNT          762 a          471 c          606 g          853 t
ORIGIN      
        1 tccctgctaa aagataagga gtttttatgt ctttactaag gcgtattgag caaggacaaa
       61 gttccaataa tcaacaacct tctagcacat caaaccaggg ggcatcaaaa caagcggaaa
      121 gtgaagcttc ttctaatgga aattcagatt cttcacgctt atcttcattg caggctcgta
      181 gagttagtgc tccaataact tccccacaag ctggctctta ttttgatctt aagactagag
      241 ttcaaaataa actattagct gaaatagacc cctcaatgga tgtaacccgt acagatgagg
      301 ttcggcgcac tattcaaaac ctgtttgaac agatattgac tgaagaaaac attgttcttt
      361 cacgcccaga gcgtgccaga ctctttgaac agatttcggc agaaatacta gggtttggac
      421 ctctacaatc cttgcttgaa gatgatacca ttacagaaat tatggtcaac ggaccgaaaa
      481 atgtttatat agaacgtaaa ggtaaagtgc atcgtgttcc cattacgttt gagagtaatg
      541 atcatgtaat gagaattatt gaccgtattg tggcaccttt agggcgtcgt attgacgagt
      601 caagccccta tgttgatgct cgtcttccag atggttcacg cgttaatgct gttattccac
      661 caatatccct agttggtcct gttttaacta ttcgtaaatt ttctaaaaat ccaattactg
      721 tcgatcaaat gattcaattt gggtcagtta gtgctgaagc agttcaattc ttgaaagctt
      781 gcgtagagtc gcgtctcaat attattattt ctggaggtac aggttctgga aagacaacgc
      841 tgttaaatgt gctatctagt tttattcctt cggatgaacg tattcttaca attgaaaatg
      901 cggcggaact tcagctacgg caagagcatg tagttactct tgagtctagg cctgctaata
      961 ttgaaggacg aggtgagatt acgattcgag atcttgttat caatgcactt cgtatgcgcc
     1021 ctgagcgcat tattgttggc gaatgccgtg ggggagagac tttagatatg ttacaggcaa
     1081 tgaacacagg tcatgatggc tcaatgacta ctgctcacgc taatactcct cgtgatgcat
     1141 tatctcgtat tgaaacaatg tgtttgatgg ctggtatgga tttgccaatt agggcaattc
     1201 gagaacaagt tgctagtgct gtggatttaa tatgccaaca agaacgtatg cgtgatggca
     1261 ctcgtaaagt aactacaata accgaagtta gtggcatgga aggtgatgtg atcacaatga
     1321 ctgatatttt tgtgtttgaa caaacaggca cagaaaatgg aagaatcatt ggaagacttc
     1381 gaccgacagg acttcgacct aagtttatgg ataaaattga agctgctggt atacatttgc
     1441 ctccctctat tttcggaatc ggcgagcgta ggcggtatta gtttatggct tctaaagtaa
     1501 attctgagga aatattacaa tgacttgggt cattatattt ggtggaatta tcataatagt
     1561 gcttcttatt gttggcgtag ttgtttcgtc taatagtgaa cgttctgccc ttgaagaacg
     1621 tttaagccaa taccttgatg cagataaaac agatgtagat aaagaggcgc aaaaatatgc
     1681 actgactaat tgggtttcta aacgtgtaga gaagacttca ttcggtgatc gtattgctcg
     1741 ccaacttgct cgagcagact taaaatttaa ggttgcagag tattttgcat taatttttat
     1801 aagtgttttt ttattctctt tagtagcatg gtttcttgga aatcgccatc cggtttcagc
     1861 gttaattggt ctagttattg gcgcaattgc acccggacag tacgtgcgtg cgcaacaaaa
     1921 acaaagatta cgtcggttta atgatcaatt gcccgatatg ctaaacttaa tggtaaatgg
     1981 acttcgtgct ggttactcaa caatgcaagc tatggaagcc attagtaaag aacttccagc
     2041 accaattaat gatgaattta aacgagttgt tcaggaaatg cagattggta tcccaatgga
     2101 aacagcactt gataacctct tgaggcgtat tcccagtgaa gatcttgatt ttgtagttac
     2161 ggcaattaat gttcagagag aagttggtgg caatctctct gagattttag ataatatttc
     2221 gtttactatt cgcgaacgta tacgtattaa aggagaggtt cgtgtattga ctgcacaggt
     2281 tcgtacttct ggtacggttt tatccttaat accatttggt ttaactataa tattatggtt
     2341 cttgaatcca gattatttat tgtctgtaac taaggggggg cccatttgta cagcagttat
     2401 tatttgtgta gttcttggtt taattatttc tagctacttt atcatgatga agattgcgga
     2461 tattgaggtg taatatggga gtagccatag cgattgttgt tggtattgcc ctgattggtg
     2521 gcatcgtttt tgttgttatt ataggggcgc gttatgcacg tgatgctcaa ggtgaagagg
     2581 cggatcctgt tttggcacgt ttggcagagg caacacaacg tggtgaaaat gtttctcttg
     2641 aagatattga acttcaacag ccattcatgg agcgtgttgt aatcccagtt gt
//
LOCUS       DAOQJE010000512         2691 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_26721_length_2691_cov_4.690061, whole genome shotgun sequence.
ACCESSION   DAOQJE010000512 DAOQJE010000000
VERSION     DAOQJE010000512.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2691)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2691)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2691
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26721_length_2691_cov_4.690061"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            215..1378
                     /locus_tag="PLT08_12665"
     CDS             215..1378
                     /locus_tag="PLT08_12665"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNE05369.1"
                     /translation="MAERILIVDDDVDTLRLVGLMLQRQGYQVIAASNGSQGLTKAFE
                     ERPDLILLDVMMPDMDGYEVTRRLRKNPATAAIPVLMFTAKTQLDDKVAGFEVGADDY
                     LTKPTHPTELQSHVKALLARAIPKRPEDMETVYREKNGYVIGVLSVRGGLGVSTVAAN
                     LAAGLYTRSKADVVLAELTPGQGSIAFEFGLSSQNGLSDILKGKSIEITREKIQSALV
                     PHNMGARILAASDSPRDVDLMTYVDNFETLILRLGGLARFIVLDLGSGLPASIQKSLS
                     LCTDRIVVLEGTASSIHQTKILINEMAELGIDAKTISVVLNNRQRSEAQIPWAQVQER
                     LGHPIVTSLTPASELFMQASKLNTPALVAQPEHMVSQQFFKLADAIIEREKAR"
     gene            1375..2151
                     /locus_tag="PLT08_12670"
     CDS             1375..2151
                     /locus_tag="PLT08_12670"
                     /EC_number="2.3.1.286"
                     /inference="COORDINATES: protein motif:HMM:NF001753.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0017136 - NAD-dependent histone
                     deacetylase activity [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent deacylase"
                     /protein_id="HNE05370.1"
                     /translation="MSTLSPQTQTNIEFAADLIRQSKHMVILTGAGVSTPSGIPDFRS
                     SGTGLWSKDEPLEVASLSTFRTKPSLFFDWFRPLARQMFDAKPNPAHMALSELEQHGY
                     VKSIITQNIDVLHQKAGSKNVIEMHGTLQTLTCTQCYHKEQAEAYIEVFVATGDIPRC
                     PKCGQVLKPDVILFGEQLPQAAWYTAQKEARTCDLMLVAGSSLEVLPVAGLPMQALDR
                     GAHLIIINNNSTYLNVRADVSILDDVATILPAITKRVFNG"
     gene            2144..>2691
                     /locus_tag="PLT08_12675"
     CDS             2144..>2691
                     /locus_tag="PLT08_12675"
                     /inference="COORDINATES: protein motif:HMM:NF024583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HNE05371.1"
                     /translation="MAEIKTERLDSYRRLIDISRDLASTLDLDILLSRIVHAAAEISG
                     SEAASILLYDDASRQLYFQVSTNMDESTRRGITVPLDGSIAGWIVTNRKPVRITNAHE
                     DERFYSNVEAVTGLSTQSLLGIPLITKNKIVGVLEALNKHKGKFSEIDESLLLVLGAQ
                     AAVAIENTRLFQQSDLISEFVH"
BASE COUNT          737 a          578 c          607 g          769 t
ORIGIN      
        1 cgcgctcggc tcgcattccg gaaccagcct ctcccgtcga agcctgtcat ccccggcttt
       61 gttgaacgac tacacaggcg ttccgttgct atattcatta tagcatgacc ccattgtgaa
      121 acttgctcac ttgtccgtta taattgaata aacaggaaag caattacttc agggctcgtt
      181 tcaaaataaa agacacctgt tcgatgaggt tgacatggca gagagaatat taattgtcga
      241 tgatgatgta gatacactga ggttggtggg gttaatgctc caacgacaag ggtatcaagt
      301 gattgcagct tcaaacggct cgcaaggact gacaaaagcg tttgaagaac gcccagattt
      361 gattctgcta gatgtgatga tgccggatat ggatggctat gaggtcactc gtcgtttgag
      421 aaagaaccct gccacggctg ctattccggt tttaatgttt actgctaaaa ctcaacttga
      481 tgataaagta gctggttttg aagttggtgc agatgattat ctgaccaagc ctacacaccc
      541 caccgaactt caatctcacg ttaaagcgct ccttgcgcga gcaataccca aacgtcctga
      601 agacatggaa actgtttaca gggaaaagaa tgggtatgta attggtgttc tttctgtgcg
      661 cggtggattg ggtgtttcta cagttgcagc aaacttggca gcaggtttgt acacccgcag
      721 caaagcagat gttgttttag ccgagcttac acccgggcaa ggttctattg cttttgaatt
      781 tgggttgtct tcacaaaatg ggctttcaga tatccttaag gggaagtcta ttgaaattac
      841 acgtgaaaag atccaatctg cattagttcc acacaatatg ggggctagaa ttctggcagc
      901 ttcagatagt ccgcgcgatg tagatttaat gacctatgtg gataattttg agacccttat
      961 tctccgttta ggtgggcttg ctcgttttat tgttcttgac cttggttctg ggcttccagc
     1021 ttcaattcaa aagagccttt ctctttgtac tgatcgtatt gttgttcttg aaggtacagc
     1081 cagttcaatt caccagacaa agattctcat aaacgaaatg gctgaattgg gcattgatgc
     1141 aaagacaatt tctgtggttc ttaataatcg tcaacggtca gaagctcaaa tcccatgggc
     1201 gcaagttcaa gaacgcttag ggcaccctat tgttacatca ctgacaccag cctcagagct
     1261 gttcatgcaa gcgtccaaat tgaatactcc agcgttagtt gctcagccag aacatatggt
     1321 ttcgcaacag ttctttaaac tagcagatgc catcatagag cgtgaaaagg ctcgatgagc
     1381 acgttatctc cccagactca gacaaacatt gaatttgcag cggatctgat tcgtcaatcc
     1441 aaacacatgg ttattctgac tggggcaggg gtttcaacac catcgggcat tccggatttt
     1501 cgttcatctg gaacaggctt atggtccaaa gacgagccgt tggaagtagc atcattaagc
     1561 acatttcgta ccaagccaag cttatttttt gattggttcc gccctttggc tcggcaaatg
     1621 tttgatgcaa aacccaaccc tgctcatatg gcactatctg aattggaaca acatgggtat
     1681 gtaaaatcca ttattaccca aaacattgat gttcttcatc aaaaagctgg atccaaaaat
     1741 gtaattgaaa tgcatggaac cctgcaaact ttgacttgta ctcagtgtta tcataaagag
     1801 caggcagaag cgtatattga agtgtttgtt gcaacaggtg atatcccccg ttgtccaaaa
     1861 tgtggacaag tattgaagcc tgatgtgatt ctttttggag aacaacttcc acaagcagct
     1921 tggtatacgg ctcaaaaaga agctcgtaca tgtgacctga tgttagtagc gggttcttca
     1981 cttgaggtgt tgcctgtggc agggcttcct atgcaagcct tggatcgtgg agcacatttg
     2041 attattatta ataacaattc tacgtattta aatgttcgcg cagatgtttc cattcttgat
     2101 gatgtagcaa ctattctgcc ggcaatcaca aagagagttt tcaatggctg aaattaaaac
     2161 agaacgatta gacagttatc ggcgtctcat cgacatctca cgcgatctcg cctccacact
     2221 agatttagat attctgcttt cacgtattgt gcatgctgca gcagaaatca gtgggtcaga
     2281 agcagcatcc attcttctgt atgatgatgc gtcacgtcaa ttgtattttc aagtttcaac
     2341 caatatggat gaatccactc gccgtggaat taccgttccg ctggatggaa gcattgcagg
     2401 ttggatcgtc acaaaccgca agccagttcg tatcaccaac gcgcatgaag atgaacgctt
     2461 ctactcaaat gtggaggcgg tgacaggtct ttctactcaa tcattgcttg gaattcctct
     2521 gattacaaaa aataagatag ttggcgtatt agaggcacta aataagcata agggaaaatt
     2581 ctctgaaatt gatgaatccc tattgttagt gttgggcgct caagcggctg ttgcgattga
     2641 gaacacccgg ctcttccaac aatcggacct gatctctgaa ttcgtgcatg a
//
LOCUS       DAOQJE010000513         2687 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_26776_length_2687_cov_9.235562, whole genome shotgun sequence.
ACCESSION   DAOQJE010000513 DAOQJE010000000
VERSION     DAOQJE010000513.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2687)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2687)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2687
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26776_length_2687_cov_9.235562"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            52..630
                     /locus_tag="PLT08_12680"
     CDS             52..630
                     /locus_tag="PLT08_12680"
                     /inference="COORDINATES: protein motif:HMM:NF015125.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPCC family cysteine-rich protein"
                     /protein_id="HNE05372.1"
                     /translation="MSEKYICPCCNFRTLDEPPSGTFNICPICFWEDDNVQLKNPDFA
                     GGANQASLNQARENFREFGACELRCKEHVRKPTIEEIFEKLSRVSGQPIDIEAELTLI
                     SPENGGRKNHFFCGMRPQFYYDNQDWDCTLQILNDPIFAHGQTITAFFGFVTPSAHLG
                     KLVPEKEFLLRDGHKIIAQGRVKVVWSLARAK"
     gene            841..1911
                     /locus_tag="PLT08_12685"
     CDS             841..1911
                     /locus_tag="PLT08_12685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE05373.1"
                     /translation="MKLQRLNLIITCFLFILLHTACNGQSTIAPTATNTQTKTLIPTK
                     TPTLISTPTPKASSTSNPVASTEKAGRATAIAIQTAERAASIQAWDAKETQIAEFSID
                     CDSINMYSSNISPNGKWFSASCDEKETSTLVVQNKVGTKWVLEFKDFLSKDSPEGMWG
                     GVSPVFWSPDGNFLYFTIGLGYSGGGNYCFPKNRGKYGLFRLDLLRGSWSTIISPTDN
                     FPGYEIVFSPTGRRFAISLDGVTISDLNTGENIRLGINGIVERMSWSPDGKYLAYASA
                     ICDDENIVSSSIYIWDSTTSQSQILFEADKMILRPEAWTSNSTLRIAGEEIIGFDSAY
                     TIYEYNIEPRGLLFSGTETPNP"
     gene            1927..2100
                     /locus_tag="PLT08_12690"
     CDS             1927..2100
                     /locus_tag="PLT08_12690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE05374.1"
                     /translation="MHLTLGILRTSQAVSHALSFFWLDGFAVPAPAQVTQTVGRLLSK
                     TMPSFFENIALGG"
     gene            2107..2523
                     /locus_tag="PLT08_12695"
     CDS             2107..2523
                     /locus_tag="PLT08_12695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE05375.1"
                     /translation="MDKSKYNPVVIRKAFEFLVSEFGYKIVRDEESFHENRPYAFTID
                     YVRNKRQVHLVYDYKENFFYFEIISIGAGLTDQHPNTNIRESSTIFWKLFKHFEPSLE
                     LKAIQPDGQTCEEAALVNAQLLQKYASDILRGEKWV"
BASE COUNT          827 a          536 c          582 g          742 t
ORIGIN      
        1 ccgccccagc gcaggtaacg caatccgttg ggcgcttaac gaatgaagtc tatgagtgaa
       61 aagtatatct gtccgtgttg taatttccgc acacttgatg aaccgccatc aggcacattt
      121 aatatttgcc ctatttgctt ttgggaagat gataatgtgc aattaaaaaa tcccgatttt
      181 gctggtggag caaatcaggc atcattaaat caagcgcgtg agaattttcg agagttcggc
      241 gcatgtgagt tacgatgtaa agaacatgta agaaaaccga cgatagaaga aatttttgag
      301 aaattatccc gtgtcagtgg acaacctata gatattgaag cggaattaac tttgattagc
      361 ccagaaaatg gcgggcgaaa aaatcatttt ttctgtggta tgcgtccgca gttttactac
      421 gataaccaag actgggattg cacattacaa attttgaatg accctatttt tgctcatggg
      481 caaactatca cggcgttttt tgggtttgtt accccatcag cacatttagg aaaattagtt
      541 ccagagaaag aatttttatt gcgtgatggt cacaagataa ttgcgcaagg acgtgtcaaa
      601 gtcgtgtggt ctttggcaag ggcaaaataa aaattgtgtt gtttttttag caagcaagcg
      661 ttatggtaaa ctgcgcccaa caaagcgtgc acctgacgct ggggactctg cggcaatctc
      721 aagcattttc ctacgcctga gcatttttct ggttggacgg cttcgccgtc cccgccccag
      781 cgcaggtaac gcaaaccgtt agccgcttgg ttgccaatat tgaaatcagg gaaaataaga
      841 atgaagttgc aaaggttgaa tttaattatt acgtgctttt tgtttatcct gttacatacc
      901 gcttgtaatg gtcaaagtac aattgcacca acagccacaa acacccaaac taaaacacta
      961 attccaacaa aaacccctac gctaatttca actcctacac caaaggcaag tagtacttcc
     1021 aacccagttg catccaccga aaaggctgga cgtgctacag ccatagcgat acaaacggca
     1081 gaaagagccg caagtataca agcatgggat gcgaaagaaa ctcaaatagc cgaattttct
     1141 attgactgcg atagcatcaa tatgtattca agcaacattt ccccaaatgg aaaatggttt
     1201 tctgcaagtt gcgatgaaaa agaaacttca actttagtag tgcagaacaa agtgggcaca
     1261 aaatgggtat tagaatttaa agactttctc agtaaagata gccctgaagg aatgtggggc
     1321 ggagtttcgc cagtgttttg gagtcccgat gggaattttc tttacttcac aataggatta
     1381 ggatattctg ggggaggcaa ctactgtttt ccaaagaatc gtggcaaata cggtttattt
     1441 aggcttgacc tcttaagagg tagttggtca actattatct ccccaacaga taatttccct
     1501 ggatacgaaa ttgtcttctc accaactggt aggcggtttg caatatcact agacggtgtg
     1561 acgatttcgg acttaaacac tggtgaaaat attcgattag gaataaacgg aattgttgaa
     1621 agaatgagtt ggtcaccaga cgggaagtat cttgcctacg cttccgcaat ttgtgacgat
     1681 gaaaacatag tatcatcatc gatatatatt tgggactcta cgactagcca atcccaaatc
     1741 ttatttgaag cagataaaat gatactaaga cctgaggcat ggacaagcaa ttcaacgctt
     1801 agaattgcag gagaagaaat aataggtttt gattcggcat acacaatata tgaatacaat
     1861 attgaaccga gaggtttatt attttcaggg acagaaaccc ccaatccata acgcggctaa
     1921 caaagcgtgc acctgacgct ggggattctg cgcacatccc aagcagtttc acacgcctta
     1981 tcatttttct ggctggacgg cttcgccgtc cccgccccag cgcaggtaac gcaaaccgtt
     2041 gggcgtttgc tttccaaaac tatgccgtct ttttttgaaa atattgctct aggtggttga
     2101 attcaaatgg ataaaagcaa atacaatcct gtggttattc gcaaagcatt tgaattcttg
     2161 gttagtgaat ttggctataa aatcgttcgt gacgaagaat catttcacga aaatcgtcct
     2221 tatgctttca caattgatta cgttaggaat aagcgtcaag tgcatttggt ttatgattac
     2281 aaggaaaact tcttttattt tgaaatcatc agcattggcg ctggattgac agaccaacat
     2341 ccaaatacga acatccgtga aagtagcact atattttgga aactttttaa gcactttgag
     2401 ccgtcgttag agttaaaagc aatacaacca gatggtcaaa cttgtgaaga agccgctttg
     2461 gtgaatgctc aactcttgca aaaatatgca tcagatattt tgcgtggcga aaaatgggtt
     2521 taaactggaa agtcttcaaa atcggtttga tagttgccaa agaataattt atgtgtaaaa
     2581 cttgggcgtg ttggcggcag tatctttttt cagtcaacga gtcttgcgca acaaaaaacg
     2641 cccaacaaag cgtgcacctg acgctgggga ttctgcggca atctcaa
//
LOCUS       DAOQJE010000514         2675 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_26971_length_2675_cov_5.708015, whole genome shotgun sequence.
ACCESSION   DAOQJE010000514 DAOQJE010000000
VERSION     DAOQJE010000514.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2675)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2675)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2675
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26971_length_2675_cov_5.708015"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1027
                     /locus_tag="PLT08_12700"
     CDS             <1..1027
                     /locus_tag="PLT08_12700"
                     /inference="COORDINATES: protein motif:HMM:NF012742.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HNE05376.1"
                     /translation="YQMTPNRPTIRDVARQAGVSHQTVSRVINGSEDVLPETRAVVEA
                     AIKDLGYRPSAIARSMARGLTHTLAIISPNLTDYTFASVIEGAELEARQHDYFVLSSS
                     ASNPRAFQGLVDELVGHRRVDGLIVINPYADERYQHLPKDFPVVFVGARSHDENVCSI
                     SLDDEKVAYEATQHLISLGHKRIALVTGPMEEDCSQDRLEGFQHALKEAGISFDSSLV
                     FEGDWSASSGSEALADFVRKGHLPTAIFAQNDRMAMGVMRAARDANLNVPDQLSVIGV
                     DDMPLSSYFDPPLTTMRQDMPLIGQEAIRKLIDIIQNKTVEQTVLKLPAQLVVRQSTV
                     KGGDATS"
     gene            1095..2492
                     /locus_tag="PLT08_12705"
     CDS             1095..2492
                     /locus_tag="PLT08_12705"
                     /inference="COORDINATES: protein motif:HMM:NF024808.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HNE05377.1"
                     /translation="MKKNLLVKIMGLLVIASFVLAACGTPAATEAPAATEAPAVTEAP
                     AATEAPATEASSADVALRWRTRPDNQEEIDVYSKISTELDAQLDGITLTYEPGGSEGA
                     NYQDQLRAEIAAGTAPDVFWIPGTDVADFATRGLILNAADLAANTDGFAVTDFYEGPM
                     YHLTFNPETSTTGADSGALWGLPRDVSTFALYLNMDLINEAGAPDPRELAANGEWNWE
                     SFLEVSQATRALGSDIYGYGASAWWGPYGVWLNASGGGFFNEDRTACALNTPESLAGL
                     DFQRSLYQDYDVAVPYGEDPEPGFRAGKVAMFQNGRWATPGVRTVDFNWDVVELPAGP
                     NGTPGNWLFWGAYVINANTEHPEEAWALVQALTTAETQGKISALGANIPSRVSPEALD
                     AFLTFTPPANNQAFLNGIAPDAAPTAEGPLWAGSWPEFDKIMGPAIQGVLTGSTTVDD
                     FGATICDEANKAFNQ"
BASE COUNT          565 a          722 c          698 g          690 t
ORIGIN      
        1 atatcaaatg actcctaatc gacctaccat tcgtgatgtt gcccgccagg ctggcgtttc
       61 tcaccagact gtttctcgtg ttattaacgg cagtgaggat gtgctgcctg aaactagagc
      121 tgtggtggag gcggcaatta aggatctggg ctatcgtccg agcgcgatag cacgttcgat
      181 ggcgcgcggc ttgacccata cacttgccat tatttccccc aatcttacag actatacctt
      241 tgccagcgtg atcgaaggcg ctgagttgga agcccgccaa cacgattatt tcgtgctttc
      301 ttcttcggct tcaaatccgc gggcgtttca gggcttggtg gatgagttgg tggggcacag
      361 gcgggtggat ggtttgatcg tgatcaatcc gtatgcagat gaacggtacc agcatttgcc
      421 caaagatttt ccggtggtgt tcgtaggggc gcgttcgcat gatgagaatg tctgctcgat
      481 ctctttggat gatgaaaagg tggcatacga agccacgcag catttgatct cgcttggcca
      541 taagcgtatt gctttggtca ccgggccgat ggaagaagat tgttcgcaag atcgtttgga
      601 aggttttcaa cacgctttga aagaagccgg tatttccttt gattcatcgt tggtgtttga
      661 aggggattgg tcggcttctt ccggcagcga ggcgctggcg gactttgtca ggaagggtca
      721 cttgccaacg gctatcttcg ctcagaacga tcgtatggct atgggtgtga tgcgcgccgc
      781 ccgggatgca aacctgaacg tgccggatca actctctgtc attggcgtgg acgatatgcc
      841 tttgtcttcc tattttgacc cgcctctcac gaccatgcgg caggatatgc ctctcattgg
      901 tcaggaggcc atccgcaagt tgattgacat cattcaaaac aagaccgttg agcaaaccgt
      961 tcttaaatta cctgctcagt tggttgtacg tcaatccacc gtcaaaggag gtgatgcaac
     1021 ttcttaatac tttttattgc aaacctgtct gatcgtaaga tgatttgttc gtccaattcc
     1081 aaggagaaga ataaatgaaa aagaatttgc ttgtgaagat tatggggctg cttgttatag
     1141 cctctttcgt tttggctgct tgcggtaccc ctgcagccac cgaggcccct gcggcgactg
     1201 aagcgccggc agtgacggaa gccccagcgg caactgaagc tcccgccacc gaagcatcga
     1261 gcgctgatgt tgctttgcgc tggcgcacac gcccagacaa tcaggaagag attgacgttt
     1321 acagcaagat cagtactgaa cttgatgctc agcttgatgg tatcacatta acttatgaac
     1381 ccggtggttc tgaaggtgca aattatcagg atcaactccg cgctgaaatt gcagccggca
     1441 ccgctcctga tgtattctgg atccccggaa ccgatgtggc tgatttcgct acccgtggtt
     1501 tgatcctcaa tgctgccgac ctcgctgcca acactgacgg cttcgctgtc actgacttct
     1561 acgaaggtcc tatgtaccac ctgaccttca accctgaaac cagcaccacc ggtgctgact
     1621 ctggcgctct ttggggtctt cctcgcgatg tgtcgacctt cgctctttac ctcaacatgg
     1681 atctgatcaa cgaagctggc gctcccgatc ctcgtgaatt ggctgccaac ggcgaatgga
     1741 actgggaatc cttcctcgaa gtttctcagg ctacccgcgc tctcggctct gacatttacg
     1801 gctacggcgc ttccgcttgg tggggtcctt acggcgtgtg gttgaatgct tctggcggcg
     1861 gtttcttcaa tgaagaccgc actgcttgtg ctctcaacac ccctgagtct cttgctggtc
     1921 tcgacttcca acgctccctc tatcaggact acgatgtggc tgtgccttac ggtgaagatc
     1981 ccgaacccgg cttccgcgct ggtaaggttg ccatgttcca gaatggtcgc tgggctaccc
     2041 cgggcgtccg cactgtagac ttcaactggg acgtcgttga attgcctgct ggtcctaacg
     2101 gaacacctgg taactggttg ttctggggcg cgtatgtaat caacgccaac accgaacacc
     2161 ctgaagaagc ttgggctctc gttcaggctc tcaccactgc tgaaacccag ggcaagattt
     2221 ctgctctcgg tgccaacatc cccagccgtg tgagccccga agcgctcgat gccttcctca
     2281 ccttcacccc gcctgctaac aatcaagcct tcttgaacgg catcgctcct gatgctgctc
     2341 cgaccgctga aggtccattg tgggctggtt cctggcctga attcgacaag atcatgggtc
     2401 ccgccatcca aggcgtgttg accggttcca ccacggtgga cgatttcggc gccacgatct
     2461 gtgacgaagc caataaagcc ttcaatcaat aagtaattct tgtacgactg gtggggcagg
     2521 cagtattttg cctgccccac tttgcccgat gtacgaagaa atccccaaac ttcaaaagtt
     2581 ttgcagctta gagatttttt tgtaaccgca ctgacgccca atttcacgga gatgagaata
     2641 tgacggctca atcacggata gacgctcaga cgaat
//
LOCUS       DAOQJE010000515         2675 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_26972_length_2675_cov_4.134351, whole genome shotgun sequence.
ACCESSION   DAOQJE010000515 DAOQJE010000000
VERSION     DAOQJE010000515.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2675)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2675)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2675
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26972_length_2675_cov_4.134351"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1175
                     /locus_tag="PLT08_12710"
     CDS             <1..1175
                     /locus_tag="PLT08_12710"
                     /inference="COORDINATES: protein motif:HMM:NF024808.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HNE05378.1"
                     /translation="TPWYGVEQSLFESFVNEFNSSNPYGIKVSAESQSNFTNVYEITT
                     AALPTENKPDLVIALPEHAQGWYQDQVSIDLTDYAEDPVYGLDESDIPYAIWNQDIAG
                     TARVAVPAQRTARVMLWNETWANELKIKSAPVSVDDFRKQACNAHTSMKKDAFAENDA
                     MGGWIVDTEPMTVYSWLLAFHGGVLEGENYRFLAPNNIDAFKYLRELAEAGCSWQGSS
                     DPITSFAKREALFITVSLSDLPTVARAFVAAENRDTWKVLPFPNGEDGVIAVYGSSYV
                     IFQSTEEKQMAAWLFVRWLLEKEQDARWVEATHNFPLHTSTVDLLGDYEKNHAQWRQA
                     VELLPLGELQPQLGSWRTIKLVLGDGFDHIYRVNVSSGQVAAILAQMESLAKDLNK"
     gene            1191..1967
                     /locus_tag="PLT08_12715"
     CDS             1191..1967
                     /locus_tag="PLT08_12715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE05379.1"
                     /translation="MATSDYKARQVRAKTSEVLEYENNDEKPGKPLHILIPGGLIALG
                     TLALIVFIAYLTWFDDSLADSTGTFLCLILSPFYVGGVFLFSYGYELYNIPRAIRLTA
                     IIVFVTLASVVIIAVLFFLLGNMKGGGSSSGSNNNSRSKSSSRSSKPSGSGLGGAVLG
                     GMMGSSSSNSSSSSNSSSGGHYHSSGPVFIGGLGGTRVETREVTKEVIKEVPKEPMPV
                     TCPFCTRSYIPVEHNYVCPSCGAATPKDLIEESQMPDEEK"
     gene            2020..>2675
                     /locus_tag="PLT08_12720"
     CDS             2020..>2675
                     /locus_tag="PLT08_12720"
                     /inference="COORDINATES: protein motif:HMM:NF013646.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent monooxygenase"
                     /protein_id="HNE05380.1"
                     /translation="MSNENNFDVIIVGGGPAGSTAAYLLKQQSLDVLIIDKDKFPRMK
                     LCGGLLTDKTLKILDEIYGDTIQTLIDKGVINYVSYSYELRTKDEIWNAGHSAIPSVI
                     VNRYIYDHYLLNRSLEAGAHILHQDRVTKVDISNNTVTTISGKTLKAKIIIGADGANS
                     VVRKAMNKSIYDPQKWIKGLATGLEVTIPRANVGLDVSGPIIFFDIVPWGYGWVFPNK
                     "
BASE COUNT          735 a          584 c          674 g          682 t
ORIGIN      
        1 ggacgccgtg gtatggcgtg gagcaaagtc tgttcgagag ctttgtcaac gaatttaatt
       61 ccagcaaccc gtatggcatc aaggtctcgg cggagagtca gagcaacttt acgaacgtat
      121 acgaaatcac cacagccgct ttgcccaccg aaaacaaacc agaccttgtg attgcattgc
      181 ctgaacatgc ccagggttgg tatcaagatc aggtgagcat agacctaacc gattacgccg
      241 aagacccggt ctatggattg gatgagagcg atattccata tgcaatttgg aatcaagata
      301 tcgcaggcac ggcgcgggtg gcggttccgg cacaacgcac agcacgcgtg atgttatgga
      361 acgagacttg ggcaaatgaa ttgaagatca aatctgcacc agtctctgtg gatgattttc
      421 gtaagcaggc ttgtaatgcg catacttcga tgaagaagga tgcgttcgcc gaaaatgatg
      481 ccatgggcgg ttggatcgtc gataccgaac cgatgaccgt ctattcgtgg ttgttggcgt
      541 tccatggcgg cgtgttagaa ggagaaaatt atcgttttct cgctccgaac aatattgatg
      601 ccttcaaata tttgcgagag ctggcagaag cgggttgttc ctggcaaggg tccagcgacc
      661 cgattacttc gttcgcgaag cgtgaggcgt tgttcatcac cgtcagcctg agcgatttgc
      721 ctaccgttgc gcgggctttc gtcgccgctg aaaatcgcga tacgtggaaa gtgctgccct
      781 tccccaatgg agaagatgga gtcattgccg tgtatggttc ttcgtatgtc atctttcaat
      841 caaccgaaga aaagcagatg gcggcttggc tgttcgtgcg ctggctcttg gaaaaagaac
      901 aggatgcccg ttgggtagag gcgacccaca actttccgct tcacacctcg accgtggacc
      961 tgctcggcga ctatgagaag aatcatgccc agtggcgtca agccgtggag ctgctgccgc
     1021 tcggagagtt gcaacctcag ctgggctcgt ggcgtacaat taaattagtg ttaggcgatg
     1081 ggtttgacca catatatcgt gtcaacgttt cgagcggaca ggtagcggcg atccttgcgc
     1141 aaatggaatc tttagcgaaa gacctgaata agtgatgaaa ggatacaggc atggcgactt
     1201 cggactataa agcaaggcag gtgcgggcaa aaaccagcga ggtgctggaa tatgaaaata
     1261 acgatgagaa acccggcaag cctttacata tcttgattcc cggcgggttg atcgcactgg
     1321 gaacgctggc gttgatcgtc tttatcgcct acttaacctg gttcgacgac agccttgccg
     1381 attcaaccgg caccttcctc tgcctgattc tctcaccatt ctatgtgggc ggcgttttcc
     1441 tttttagtta tggctacgag ctttacaaca ttccgcgtgc cattcggtta acagcaatca
     1501 tcgtattcgt aacgttggct tcggtggtca ttattgctgt gcttttcttt ttgcttggga
     1561 acatgaaagg cggcggttcc agttccggca gtaacaacaa cagtcgtagc aaatcgtcct
     1621 ctcggtcttc caaaccatcg ggcagtgggt tgggcggtgc ggtgctcggc ggcatgatgg
     1681 gaagttcctc cagcaattcg tcttcctctt ccaattcctc ttcgggcggg cactaccatt
     1741 caagcggacc cgtcttcatt ggcgggctgg gcggcacacg tgttgaaact cgtgaggtca
     1801 ccaaagaagt aatcaaagag gtaccgaaag aaccaatgcc ggttacttgt cccttctgta
     1861 ctcgctcgta cattcctgta gagcataatt acgtctgccc gagttgtggt gcggcgactc
     1921 cgaaagactt gattgaagag tcacagatgc cagacgaaga aaaataaacg tttaggtaag
     1981 tttagaatat atatttaaat aattaagaat agactataaa tgtcaaacga aaataacttt
     2041 gatgtgataa tcgtaggagg tggacctgct ggatcaaccg cagcatacct acttaaacaa
     2101 caaagtttag atgtcttaat tattgacaaa gacaaatttc cgagaatgaa actttgtggg
     2161 ggattattaa cagataaaac tttaaaaata cttgatgaaa tttatggcga cactatacaa
     2221 acgctgatag ataaaggtgt catcaattac gtctcctact cttatgagct tcgaactaag
     2281 gacgaaatct ggaatgccgg acacagtgca atacctagtg taatagtcaa tcgctatatt
     2341 tacgaccatt atttattaaa tcgttcacta gaagcaggag cacacatact tcaccaagac
     2401 agagtcacta aagtagatat tagtaataat accgtaacaa ctatcagcgg aaaaacacta
     2461 aaagcgaaaa ttattattgg ggcagatggg gcaaatagcg tagttagaaa agctatgaac
     2521 aaatcaattt acgatcccca aaaatggata aaaggacttg ctacaggttt agaagttaca
     2581 attcctaggg ctaatgtagg tttagatgtc tcgggtccaa taatattctt tgatattgtt
     2641 ccatggggat atggttgggt atttccaaat aaaaa
//
LOCUS       DAOQJE010000516         2664 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_27164_length_2664_cov_5.303181, whole genome shotgun sequence.
ACCESSION   DAOQJE010000516 DAOQJE010000000
VERSION     DAOQJE010000516.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2664)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2664)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2664
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27164_length_2664_cov_5.303181"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(188..538)
                     /locus_tag="PLT08_12725"
     CDS             complement(188..538)
                     /locus_tag="PLT08_12725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE05381.1"
                     /translation="MLKSIFNLSLLPRVFRLQIVLILMLFLSACATNRDSIAKGLPTP
                     DSGFTTGTTTGYSVYIWDCYQGKHIVIFNITAEFTSQDYERQESTCGDVTSIEKQLAD
                     DESKRDLDPNYFWR"
     gene            complement(602..1378)
                     /locus_tag="PLT08_12730"
     CDS             complement(602..1378)
                     /locus_tag="PLT08_12730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE05382.1"
                     /translation="MKISKLGKIAISVILLALASIRWAFHEIAVDRMDSIFMALVISA
                     LLVYILPWENIKTFKAAGIELSLEQPEIKAAISGLGLNRINDETLKLQLQKLESDIQS
                     IRGGRVLWIDDKPHKIIGERRLLRALGIQVVSAVSSVAAESILDSDNDFDLIISDVQR
                     AGESYKLNNGVDIHEGTNFIVKLRKHEDAIIQKLPVVFYAAYDWDRLVEFTRPARELL
                     PEPEISNSAIDFVPKVIRQLAKSRATVITYSSSKDPTSIR"
     gene            complement(1365..1901)
                     /locus_tag="PLT08_12735"
     CDS             complement(1365..1901)
                     /locus_tag="PLT08_12735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE05383.1"
                     /translation="MSDSISVDFHHPTNVGILTYLGSPERLAKSVSEARDLPSCSPSS
                     VNAPYMKLGSHPDLVARLWDEVTVKLPVKCQWIVYGMPVLVNPSSGIIFAFGGGTHTY
                     AFRLPPKEHQEAVQAGAKNIYQYPAIPSLKIQASTLDLDTIGKEWIFGSWLKNEENWC
                     LAAYNFADGTIWRSHENQ"
BASE COUNT          766 a          526 c          559 g          813 t
ORIGIN      
        1 acgcaaactt gcggcttacg aatggaatgg ggatgtatca tatctgatcc tttaaagtag
       61 tgtgtgatta atgagatgta tacctgtaat tgaggttttg atttatcccc cgattgacct
      121 agagccactt caaaagttat ataaaatcat gcgccgatta tcagcattta gccctgagca
      181 gacaggttta gcgccaaaag taatttggat ctaaatctct ttttgattcg tcgtctgcca
      241 gttgtttttc gattgaagtg acatctccac aagtagactc ttgcctttca taatcctgtg
      301 acgtaaattc ggcggttata ttgaatatta cgatgtgctt gccttgataa caatcccaaa
      361 tatagacact gtaccctgtg gtagtacctg ttgtaaaccc tgaatcggga gtaggcagcc
      421 cttttgcaat gctatctctg tttgtagcac atgctgagag aaaaagcatt aagataagta
      481 cgatctgcag gcggaagact ctaggtagaa gtgaaagatt gaaaatggat ttgagcatga
      541 tttctttaag ccatctatac atagataaga taatactata tttatgtgcg tcgtgtaatt
      601 tttatctaat tgatgttggg tctttactgc tactgtaagt tatcacggtt gcccttgatt
      661 tagccaattg ccttattacc tttggaacaa agtcaattgc tgagttgcta atttcaggtt
      721 ccggtagtaa ttcgcgtgcg ggtcgcgtaa attccactag ccgatcccaa tcatatgctg
      781 cataaaatac tacaggaagc ttttggatga tagcgtcttc atgctttctg agcttaacga
      841 taaagtttgt cccttcgtgg atatcaacgc cattgtttag cttatagctc tctcctgcgc
      901 gttgtacatc ggagatgatc agatcaaaat cattatctga gtctaatatg ctttcagcgg
      961 ctacgcttga aacggctgag acgacctgta tacctaaggc tcttaatagg cgtctttcgc
     1021 caattatttt gtgaggcttg tcgtcaatcc ataaaaccct gccgccgcga atggactgta
     1081 tatcactctc gagtttttgt aactgtaatt ttaatgtttc atcattgatt cgattaagac
     1141 ctaagccgct aattgcggct tttatttcag gctgttcaag cgaaagttca atgccggctg
     1201 ctttgaatgt tttgatgttt tcccaaggaa ggatgtagac aagtaacgcg gatatgacaa
     1261 gagccatgaa gatcgaatcc atacgatcaa ctgcaatttc atgaaacgcc catcttatac
     1321 ttgctaatgc cagcaaaata acggatatcg ctatttttcc aagtttactg attttcatga
     1381 gacctccaaa ttgttccatc tgcaaaattg taggcagcaa ggcaccaatt ttcttcattc
     1441 ttgagccaag aaccgaaaat ccactctttt cctatcgtat ctagatcgag agttgaagct
     1501 tgaattttta ggcttggaat tgctgggtat tgatagatgt tctttgcacc cgcttggact
     1561 gcttcttgat gttctttagg tggaagccga aatgcgtatg tatgagtgcc gccaccaaaa
     1621 gcgaaaatga ttcctgacga aggattaact aggacaggca tgccatacac aatccattga
     1681 cacttaacgg gcaacttcac cgtgacttca tcccaaagtc gtgctactag atcgggatga
     1741 gagcctaact tcatataggg agcatttaca ctagaaggag aacaacttgg aagatcgcgt
     1801 gcctcagaca ctgactttgc tagtcgttct ggagatccta gatatgtcag gatgccgaca
     1861 tttgttggat gatggaagtc gacggaaata gaatcactca ttctaaaact ccttctctac
     1921 gcactcaaag gtttgtgttg tttttactga aatgcgtgtg agtaatgctt cggaacttca
     1981 cgtgccaggt agtatcttgc tgcccgcttt attggtcagc ggtttttata gcgatgttat
     2041 cgcatggaac gatcatatcc tgctttttct tttctatgca aatgattgag ctgccagtgc
     2101 cattaaacaa attccaataa aacctaacag gcaaataaga ataaaggtaa tgcccaaaaa
     2161 tcgcaatttt gcctttctcg atgcaaacaa gcctcccaca actccgtaga aaaggcttga
     2221 gattagaata taaggaaagg tgtatgatgt ccaatcaaca agaatcattt cgtcagtaat
     2281 gtacctccac agaaaaaagt ttattatggt agtcagaagg tagctaggag ctgtgagtat
     2341 caacggaata atagacccaa aactggcaaa agctgatgcc cataagagat aagtaagcac
     2401 aaaatgtata caagcaatcc caaaacctat cagtaagaca cggtgtttca aattattcag
     2461 agaatgcaga tatggtttca ttttcataac atccttataa agttcatgtc tacgactggg
     2521 gggaggctga ttttttttcg caatcgtaat ttattgtctt gaaagatttt ctgacgaata
     2581 gaaattatta taggtgttgc caagctatga aaatatttgg attagccgcc caacggattg
     2641 cgttacctgc gctggggtgg ggac
//
LOCUS       DAOQJE010000517         2660 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_27234_length_2660_cov_5.730902, whole genome shotgun sequence.
ACCESSION   DAOQJE010000517 DAOQJE010000000
VERSION     DAOQJE010000517.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2660)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2660)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2660
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27234_length_2660_cov_5.730902"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..933)
                     /locus_tag="PLT08_12740"
     CDS             complement(<1..933)
                     /locus_tag="PLT08_12740"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF012492.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HNE05384.1"
                     /translation="MNFNNFNLDPRLKQGIMKAGYETPTPIQEAAIPAAIRGRDIIGT
                     AQTGTGKTAAFVLPILNKLLNGQRNTPRALIVTPTRELAEQINDVIKTLSAGTKLKSA
                     TIYGGVGANPQIQALRNGAEILVACPGRLLDLIAQGHAKMANIEILVLDEADRMFDMG
                     FLPDVRRIVKAVPEKRQTMMFSATFPPDVELLAKQALREPQKIAMGIVKPAHTVEHAL
                     YPVPQHLKTKLLIELLKRTDTNSVIVFTRTKHRAHRVSEQIKKSGFKATSLHGDRTQG
                     QRQAAIKGFKSGTHDIMVATDIAARGLDIETVSHV"
     gene            complement(1004..1870)
                     /locus_tag="PLT08_12745"
     CDS             complement(1004..1870)
                     /locus_tag="PLT08_12745"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HNE05385.1"
                     /translation="MRRLTGIILIAISAASFGTLAIFGRYAYAENIDTFTLLFLRFGF
                     SASFMTMMLILRKEPFPRGRVLAQLIGMGGIGYAGQSFLYLTAIKYASAGLVALLLYL
                     YPFFVAILSMIFLQEKLTRVKAFALALALCGTALTVGPVSGQPIGAIMAIVAALGYSI
                     YIIVGANVMKHVSPFQSSTVIFTSASVVYGLLTFTNGIHLPQTNFGWLVIAGIVVVST
                     IIPVTTFLAGLERIGPTNAAMLSTIEPIITVFLATVLFGDKLAPIVLAGGALILIAVV
                     ILTRSEMRSIET"
     gene            complement(1957..2550)
                     /locus_tag="PLT08_12750"
     CDS             complement(1957..2550)
                     /locus_tag="PLT08_12750"
                     /inference="COORDINATES: protein motif:HMM:NF017519.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiiX/YebB-like N1pC/P60 family cysteine
                     hydrolase"
                     /protein_id="HNE05386.1"
                     /translation="MTVYSYQINGMSVQTGDIICTMNGKPDILPGEFWRLVGRLVPGD
                     VDHVAIYLGPDGRCAEAGAKGVITFDVPNGIWNTERMARQRGLLFDTFYGVVSPLDVL
                     GYMEEDEFHLRKAVAQYILAQVGKPYNLNFFNSETEEAFYCSQLAYKAYQTIGINLNT
                     GLAMEQLPGTNQIVYPQEIWNGFSHHKINLFRAAVSA"
BASE COUNT          690 a          624 c          687 g          659 t
ORIGIN      
        1 aacgtgtgaa acggtctcaa tatccaagcc gcgggcggca atatcggtgg caaccataat
       61 atcgtgggtt ccgcttttga agcctttgat cgcagcttga cgttgccctt gggttcggtc
      121 accatgcaag ctggtggctt tgaatccaga tttcttaatt tgctccgaca cccgatgagc
      181 acggtgtttg gtgcgtgtaa aaacgatcac cgagttcgtg tcggtacgct tgagcaattc
      241 gatcagcaat tttgttttca aatgttgcgg cacggggtaa agcgcatgct ccactgtgtg
      301 cgccggtttg acaataccca tcgcgatctt ctgtggctct cttaaagctt gctttgccag
      361 caattccaca tcaggcggga aggtagcaga gaacatcatc gtctgtcgtt tttcgggcac
      421 cgctttcaca atgcggcgta catcgggcag aaagcccata tcgaacatac ggtcggcttc
      481 atcgagcacg agaatttcaa tatttgccat cttggcatga ccttgtgcga tgaggtccag
      541 cagacgcccc gggcaagcca ctagaatttc agccccgtta cgcaaggctt ggatttgtgg
      601 gttcgctccg actccgccat agatggttgc gctcttcagc ttggtgcccg cggataacgt
      661 tttgatgaca tcgttgatct gctctgctaa ttcacgcgtc ggtgtgacga tgagtgcacg
      721 cggcgtgttg cgttgaccat tgagcaattt gttgagaata ggcaatacaa aggctgcagt
      781 tttgccggtg ccagtttgtg ctgtaccaat aatgtcacgt ccgcgaatgg cggctgggat
      841 ggcagcttct tgaatggggg tgggggtctc ataccccgcc ttcataattc cctgcttgag
      901 gcgggggtcg agattaaaat tgttgaagtt catgatttcc tttttaggtg acagtcacta
      961 tacgtgatcg tcccctgatt ctttgtatga agcattatat cgcttatgtt tcgattgaac
     1021 gcatttctga gcgtgtaaga atcacaactg caatgagaat cagtgctccg ccagccaata
     1081 cgatcggtgc aagtttgtcg ccaaataaga ccgttgcgag aaaaaccgtg atgatgggtt
     1141 caatagtcga gagcattgcc gcattggtag gaccgatccg ttccaaacca gcaagaaaag
     1201 ttgtgactgg gataatggtg gacacgacaa caatgcccgc aattaccagc caaccgaagt
     1261 tcgtctgtgg aagatgaatt ccgttggtga aagtcaacag tccatagacc acgctcgcgg
     1321 aagtgaaaat aacggtcgac gactgaaatg ggctgacatg tttcatcaca ttggcaccga
     1381 cgatgatgta aatggaatac cccaatgcgg caacaatcgc cataattgcg ccaatgggct
     1441 gtccactgac gggaccgacc gttagtgcgg tgccgcataa tgccaaggca agcgcaaagg
     1501 cttttacgcg ggtgagcttc tcttgcaaga agatcatcga taagatggcg acaaaaaagg
     1561 gatacagata caaaagcaag gcgaccaaac cggcagaagc atatttgata gcggttagat
     1621 atagaaacga ttgccctgca tacccaatgc cacccatgcc gattagttga gcgaggacgc
     1681 gtccgcgtgg gaaaggctcc ttgcgcagaa tcaacatcat ggtcatgaat gaggcggaaa
     1741 atccaaaacg gagaaaaagg agggtgaagg tgtctatgtt ttcggcgtag gcatagcgcc
     1801 caaagatggc aagcgtccca aatgatgcgg cagaaatggc gatcaaaatg attccggtga
     1861 gtcttctcat agcgggcaac tatacctcac tcaatcgtaa attgtgcttt ttaaatgcag
     1921 tatatacccg aaaaaaactc cccgttgaag gggagtttat gctgaaaccg cggctcgaaa
     1981 cagatttatt ttgtgatgtg aaaacccatt ccaaatctct tgcggataaa cgatttgatt
     2041 tgtgccaggc agttgctcca tggcgagacc cgtgttcaag ttaatgccga tggtttgata
     2101 ggctttgtat gcgagctggc tacaataaaa agcctcttcg gtttcggagt taaagaagtt
     2161 tagattgtag ggcttaccaa cttgcgcaag gatatattgg gcaacagctt tgcgtaaatg
     2221 aaattcgtct tcttccatat acccaagcac atccaatgga gagaccacac cgtaaaaggt
     2281 gtcgaacaac aaaccgcgtt ggcgtgccat acgttcggtg ttccaaattc cgttgggtac
     2341 atcaaaagtg atcacacctt ttgccccagc ctcggcgcag cgcccgtcgg gtccgaggta
     2401 gatggcgaca tggtccacat cgcccggcac gagccgccca accaagcgcc aaaactcacc
     2461 cggtaagata tcgggcttgc cgttcatggt gcagataata tcgcctgttt gcacagacat
     2521 accgttgatc tgataactgt aaaccgtcat gttactttat acgaaggtgt acagcaaaag
     2581 tttctatata aatgaaagta gaaaatctcg aatctgattc gcggtctgat tccacttggg
     2641 ctggcgctga tagcgactcc
//
LOCUS       DAOQJE010000518         2656 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_27307_length_2656_cov_5.422145, whole genome shotgun sequence.
ACCESSION   DAOQJE010000518 DAOQJE010000000
VERSION     DAOQJE010000518.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2656)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2656)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2656
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27307_length_2656_cov_5.422145"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..692
                     /locus_tag="PLT08_12755"
     CDS             <1..692
                     /locus_tag="PLT08_12755"
                     /inference="COORDINATES: protein motif:HMM:NF016800.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HNE05387.1"
                     /translation="VFFYGKKWQPFVAGIVIVPTFLALLLTFSRSAWVALFVAVSFFL
                     VCEIVLKRWNGMKRLAVLSTVTLLIVMPFIWKNFSVFGQRVNSGNVAQDQPMNERAYL
                     LDAGNTLFVEHSVLGVGLGASPLAMKMRFENFRINYQPPHFTPLLSALETGVVGGGFY
                     LLLFLAPGVTFLLRWRHYIQYPLVLGAFSLLIAISVVNLFDYYTWMYAPGRLWQWLAW
                     GLYSMTLEQAG"
     gene            694..2190
                     /locus_tag="PLT08_12760"
     CDS             694..2190
                     /locus_tag="PLT08_12760"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNE05388.1"
                     /translation="MFQYFLAPVVMIYTMTMLTLAIYIGNLLYLMSISLKNRNKIELV
                     TSQKLERFPVVTVQLPIYNEWYVAERLLDACVALDYPKDLFEIQVLDDSTDETTTLIA
                     QKVKSIQQTGVNIVHIHRTNREGFKAGALANGLATAKGEFIAIFDADFLPQKDFLLQL
                     LPRFDHEKVAFVQARWGHLNRDYSLLTMLQSFSLDAHFAIDQLGRSCADYVFNFNGTA
                     GVWRKSAILDVGGWHADTLTEDMDLSYRTFLKGWSARYAGDVEAPAELPVSFTAYRRQ
                     QYRWARGSLECAIKYIPVIWRGDFPFARKLQATFHLTGYLLHMLTVLLMLCYPLLLVF
                     ARQFPTLLTPMGIGLLMNFLAIVPALYFIVAQHLLKRHWLRSLPTILLLSVFASGMVL
                     NTMRALYQILQKHAVPFERTPKFGITERSQSWRGNRYIIRFDALVLLEVLLGCVNLGS
                     AWMALSSGYYLMMIYAFLFALGLFFFSGFTLFQSISARFYPDPKPASA"
     gene            2203..>2656
                     /locus_tag="PLT08_12765"
     CDS             2203..>2656
                     /locus_tag="PLT08_12765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE05389.1"
                     /translation="MSSNFSSSLERAILETLAYSDVFDYPLTLDELHRYLTYPATKEE
                     IMNCLNSSLSVSSNSNFYFLPKRDPLVDIRCERKTLSLPAFRRAILYGRILGMFPFVR
                     MVSMTGSLAVLNLSKDADMDYMFVTQPSRLWLARSFAVTFGRIMRLFGD"
BASE COUNT          582 a          615 c          571 g          888 t
ORIGIN      
        1 ctgttttctt ttatggcaaa aaatggcagc cttttgttgc tggtatagta attgtgccaa
       61 cctttttggc tttgttatta accttttctc gctctgcttg ggtggcttta ttcgtggcag
      121 taagcttttt cttggtttgt gaaatagttt tgaaacgatg gaatggaatg aaaagacttg
      181 ctgttctaag cactgtaact ttgctgatag ttatgccttt catatggaag aatttctcgg
      241 ttttcgggca gcgagtcaat tcagggaatg tggcgcaaga ccagcccatg aatgaacgag
      301 cctatttgct tgatgcaggc aacactcttt ttgttgagca ttctgtgctt ggagtaggtc
      361 tcggggcttc gccgcttgcg atgaaaatgc gctttgaaaa ttttcgaatt aattatcaac
      421 ctccgcattt tacgcccttg ctttcggctt tggaaaccgg cgtagttggc ggcgggtttt
      481 atcttctctt gtttcttgct cccggtgtga ctttcctgtt gcgctggcga cattacattc
      541 aatatccact tgtattggga gctttttctt tattgattgc tatttccgtt gtaaatttat
      601 ttgactacta cacttggatg tatgccccag ggcgtctttg gcagtggctg gcatgggggc
      661 tatactctat gacactagag caagcaggct aatatgtttc aatactttct tgctcctgtg
      721 gttatgatct acactatgac aatgttgacc ttggcgattt acataggcaa cctcttgtac
      781 ttgatgtcaa ttagcttgaa gaaccgtaac aaaattgagt tggtcacttc ccaaaaactg
      841 gagaggtttc cagtcgttac ggtgcaattg ccaatctaca atgagtggta tgtggcggag
      901 cgtttattag atgcttgtgt tgcactggat tatccaaaag acttattcga gattcaagtt
      961 ttggatgact caacagacga aactactact ctcatcgctc aaaaagtaaa aagcattcag
     1021 caaacgggtg tgaacattgt ccatatacac cgcacaaacc gagaaggttt caaagcaggc
     1081 gctctcgcca atggactagc aaccgccaaa ggcgaattta ttgccatttt tgatgcagat
     1141 tttctccccc aaaaagattt tcttctgcaa ctgttaccac ggttcgacca tgaaaaggtt
     1201 gcctttgtcc aagctcgttg ggggcatctc aaccgagatt attccctact aaccatgctc
     1261 caatcctttt cactggatgc gcattttgcc atagaccaac ttggacgttc ctgtgccgat
     1321 tatgttttta atttcaatgg cacagcaggg gtgtggcgta agtctgctat tcttgatgtt
     1381 ggcggttggc atgctgatac tttgacagaa gatatggacc tgtcctatcg aacattccta
     1441 aaaggctggt cggctagata cgctggggat gtggaagcac cagctgagct gccggtcagt
     1501 ttcacagcat atcgccgtca acaatatcgc tgggcgcgtg gaagtttaga gtgcgccatc
     1561 aaatacatcc ctgtgatctg gcgtggcgat tttccctttg cgcgcaaact gcaagctaca
     1621 ttccacctca ccgggtattt gttgcatatg cttaccgtct tgcttatgtt atgctatcca
     1681 ctgttgctag tgtttgcgag gcagtttcct accttgttaa cgcccatggg tattggttta
     1741 ctaatgaact ttcttgctat tgttccggct ttgtatttta ttgtggctca acatttgctc
     1801 aaacgtcact ggctacggtc tttgccaaca attcttttgc tctctgtgtt tgcttctggc
     1861 atggtgctaa atacgatgcg cgccttatac caaattttgc aaaaacatgc tgtcccattt
     1921 gaacgcaccc caaaatttgg tatcaccgaa cgctcacagt cttggcgtgg caatcgctac
     1981 atcatccgtt ttgatgcttt agtccttctt gaagttttac ttggctgtgt caaccttggg
     2041 tccgcttgga tggctttgtc ttctggttat tatttaatga tgatctatgc cttcttattc
     2101 gcgttgggat tatttttctt ctcaggcttt actctattcc aatccatttc tgctcgcttc
     2161 tatccagacc cgaaacctgc ttctgcttaa aaggcattga ccttgtcttc gaacttttct
     2221 tcctctcttg aacgcgccat cctcgaaacg ctagcgtact cggatgtctt tgattacccg
     2281 ttgaccttgg atgaattgca tcgctacttg acctatcccg ccacaaaaga agaaattatg
     2341 aattgtttga actcttcttt atctgtctca tcaaattcca atttttattt tctccccaaa
     2401 cgtgaccctc ttgtagatat tcggtgtgaa cgcaaaactt tgtctctgcc tgctttccgc
     2461 cgcgccattc tatacggacg tattcttggt atgtttccat ttgttcgaat ggtatctatg
     2521 actggctccc tcgccgtcct taacctctcc aaagatgcag atatggacta catgttcgtc
     2581 acacaaccaa gtcgcttgtg gcttgcccgc tccttcgctg tgacttttgg tcgcatcatg
     2641 cgtctctttg gcgacc
//
LOCUS       DAOQJE010000519         2653 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_27367_length_2653_cov_6.346805, whole genome shotgun sequence.
ACCESSION   DAOQJE010000519 DAOQJE010000000
VERSION     DAOQJE010000519.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2653)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2653)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2653
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27367_length_2653_cov_6.346805"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1565
                     /locus_tag="PLT08_12770"
     CDS             <1..1565
                     /locus_tag="PLT08_12770"
                     /inference="COORDINATES: protein motif:HMM:NF012791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HNE05390.1"
                     /translation="MGRSNQLLSYDDVAKKLHLQSRTERGLKNIPLDAIVGSVGRYTD
                     FTRTFLPLQDSDRERWASVKAAMVTGAGLPPIEVYQVGDVYFVLDGNHRVSIARQEGL
                     KSIEAYVTEFRTDVKLTPETKLDEIIIKAEYIEFLEKTDLAQARPNVDLSVTIPGQYE
                     KLMEQIDVCKYMDENETLTLQDAAANWYDTLYIPLAEAIRDRELLQWFPGRTITDLYI
                     WISENRAALEKELGWELRSDVAATDLILKRSVASESGSWRKARTIARYTDHLFEDILI
                     PLRGQTESWDALYQGIVIAQRDQARLHGLHIAKTKEEAEGAEAQEIKERFERVCKDAG
                     VQGSLAIDVGDITQRIIERAVVTDLIIVKLLNPPGSGLSVLNSPFRAIIEKSSRPLIT
                     VPKGATPFKRAMLAFDGSDLAKEALFVTAYLAEMWKTEVTVFTAREEKSPQGDLQDYA
                     RRYMELHEVEAKYVVSEQKTPRHLPDIAAEYNADLILMGSHSGNKLQQVLVGSMVDIT
                     LRESSIPAFICR"
     gene            1647..>2653
                     /locus_tag="PLT08_12775"
     CDS             1647..>2653
                     /locus_tag="PLT08_12775"
                     /inference="COORDINATES: protein motif:HMM:NF020028.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE05391.1"
                     /translation="MTVSASRWDLTNVYPSLDSKKFKAATKKYKSLLDELDAFLKKAE
                     KANSKTEPKKLGKLLGEAVDRFNELFELSGTMSSYLYSFITTDSRNKDAMRIFSEFQQ
                     MSVQGSILQTKFTAWAGTLGKPAIKKAVKTNASAKAHEFILLESAEQSKYLMSEAEET
                     LAAELTLSGGSAFGKLQGTLTSQMTVDFELDGEVKKMPMPALINLRSHPDESVRRRGY
                     EAENKAWADVQETLAACLNGVKGETLVLDRKRGREDNVHASIDAARIDRATLEAMLGA
                     MRDSFPMFRRYFNHKAKLIGKEKLAWWDLFAPLGKTDKVYSWDEARDFIVTNFNKFSP
                     EL"
BASE COUNT          713 a          718 c          642 g          580 t
ORIGIN      
        1 ttatggggcg ttccaatcag ttgttatcgt atgacgatgt cgcaaaaaaa ttacacctgc
       61 aaagccgcac tgagcgtgga ctgaagaaca ttccgctcga tgcaattgtt ggcagtgtgg
      121 ggcgttatac cgacttcacc cgcactttct taccgttgca ggatagcgac cgcgaacgtt
      181 gggcaagcgt caaagccgcc atggtcaccg gagcggggct tccacccatt gaggtgtatc
      241 aggttggcga tgtgtatttc gtgctcgatg gcaatcatcg cgtctcaatt gcaaggcagg
      301 aaggcttgaa atccatcgaa gcctatgtca ccgagtttag aaccgacgtc aaactcacgc
      361 cagaaaccaa attagacgaa atcatcatca aagccgaata catcgaattc ctcgaaaaga
      421 ccgatcttgc tcaagctcgc cccaatgtgg acttaagtgt caccatccct gggcaatacg
      481 aaaagttgat ggaacaaatt gacgtttgca aatacatgga tgagaacgaa accctcacgc
      541 ttcaagacgc cgccgccaac tggtacgaca ccttatatat tcccctcgct gaagccattc
      601 gcgaccgtga actactgcaa tggttccctg gtcgcaccat caccgactta tacatttgga
      661 tatcagagaa ccgtgccgcc cttgagaaag agcttgggtg ggagttaagg tctgatgtgg
      721 cagctacaga cctaatcttg aaaagaagcg tggcatctga atcgggtagt tggcgcaaag
      781 cccgcaccat cgcgcgttac accgatcatc tctttgaaga tatcctcatc cccctcaggg
      841 gacaaaccga aagctgggat gcgctttatc agggcattgt gattgcacaa cgtgaccaag
      901 ccaggctgca cggtttacac attgcgaaaa caaaagaaga agccgaaggg gctgaagcgc
      961 aagaaatcaa agaacgcttt gaacgagtct gcaaagatgc aggtgtacaa ggcagcctcg
     1021 ccattgacgt gggcgacatt acgcaacgca tcatcgaacg cgccgttgtc acagacctga
     1081 tcatcgtcaa attgctcaac ccgccagggt cggggttatc ggttttgaac tcgcccttcc
     1141 gtgccattat tgaaaaatca tcccgcccgc ttatcaccgt gcccaaaggc gccacacctt
     1201 tcaaacgagc catgcttgct tttgatgggt ctgaccttgc caaagaagcg ctcttcgtca
     1261 ctgcttatct tgccgaaatg tggaaaaccg aagtgaccgt gttcactgcc cgcgaagaga
     1321 aaagcccgca aggcgatttg caagattatg cccgccggta tatggaattg catgaagtgg
     1381 aagccaagta tgtggtgagt gaacaaaaaa ctccgcgcca cctgccagat attgccgctg
     1441 aatacaacgc agacttgatc ctaatgggca gccacagcgg aaacaaacta caacaggtcc
     1501 tagtcggcag catggtagat atcactttgc gcgagtcgag cattcctgct ttcatttgcc
     1561 gctagggttt aatccctacc agagcagggg gaaggtaaaa atcaatcacg gtatactaag
     1621 cccatctttc aacaaggagc tttcgaatga ctgtttctgc ctctcgttgg gacctgacca
     1681 acgtctaccc atctcttgat tccaaaaaat ttaaagccgc cactaagaaa tacaaatccc
     1741 tgctcgatga gctggatgcc tttctcaaga aagccgagaa agccaactcc aagaccgagc
     1801 cgaaaaagct cggcaaattg ctcggcgaag ctgtagaccg cttcaatgag ctatttgagc
     1861 tttctggcac catgagttcc tatctttact ctttcatcac aacggactca cgtaacaaag
     1921 acgccatgcg cattttctct gagttccagc agatgagcgt gcaaggcagc attttacaaa
     1981 ccaagttcac tgcctgggca ggcacacttg gcaagcccgc catcaaaaaa gcggtaaaga
     2041 ccaatgcttc tgccaaagcg catgaattca tcctgcttga atcggcggag caatccaaat
     2101 acttgatgag cgaagctgag gagaccctcg ccgccgaatt gaccttgagc ggtggcagcg
     2161 ccttcggcaa actgcagggg acgcttacct cccaaatgac cgtggacttt gagctggacg
     2221 gcgaagtgaa gaagatgccc atgccggctc tcatcaactt gcgctcacac cccgatgaat
     2281 cggttcgccg ccgcggctac gaagccgaga acaaggcttg ggcagacgtg caggaaactc
     2341 tcgccgcttg tttgaatggc gtcaaaggtg aaaccctcgt tctcgatcgc aaacgcggac
     2401 gtgaagataa cgtccatgct tccatcgacg ctgcccgcat tgaccgtgcc accctcgaag
     2461 ccatgttggg tgccatgcgc gattccttcc ccatgttccg ccgttatttc aatcacaagg
     2521 caaagttgat cggaaaagaa aaactcgctt ggtgggatct attcgctccg cttggcaaga
     2581 ccgacaaagt ctattcgtgg gatgaagccc gcgacttcat tgtgaccaac ttcaataagt
     2641 tctcacccga act
//
LOCUS       DAOQJE010000520         2653 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_27368_length_2653_cov_5.868360, whole genome shotgun sequence.
ACCESSION   DAOQJE010000520 DAOQJE010000000
VERSION     DAOQJE010000520.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2653)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2653)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2653
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27368_length_2653_cov_5.868360"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..893)
                     /locus_tag="PLT08_12780"
     CDS             complement(<1..893)
                     /locus_tag="PLT08_12780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE05392.1"
                     /translation="MRTSDSNKIMWVAAIGAAIGLIAASFYLPGGDDLYRYYLPFAQG
                     CLHCGFMPYFAQWFLAPLRWLPEYPFSWGVWSLFSVSGILILAYYTKANPFLLLISFP
                     ALGQVWLGQVDIFIATGLVVFLFNKNPYLRGLGLIVALSKPQLTALPIFFCLLMEEDP
                     RLFLKLFLIPSIIILLSFIFFGVNWIPQWILETRSNLPIHVWRLASLDVWKFGIFLTP
                     IPILFREKRDRMLSSLLISALATPFYGVYSYLVFLLFEVKWWHVVLSFAWMIGFLFLH
                     DSAMRLAWILPLAMLVEMLYK"
     gene            903..2327
                     /locus_tag="PLT08_12785"
     CDS             903..2327
                     /locus_tag="PLT08_12785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE05393.1"
                     /translation="MNSENKSLEKFLRSISFLLTFFLLSRAPIDADLWWHLRAGQLMW
                     QKGEILLNDVFSYTKLGSNWVNAFWVSEVLLYWIHRIGGYFLLSFFVSLIGAITFLIV
                     FSSMKGNSLLRSFILILAVITAAPIWGPRPQILSFFLVAFLDLWLNQPSRHRWLLIPI
                     FALWANIHGGWIWGFLLLIAHIAGLFVNSFFVEDKKKLRTEGLHLIGWTVLSALAVGI
                     NPNGIYIWKLPFQQVNVSLQIQEWLSPDFHRLDFHPMLWMLFLLILLAPFAQKPLQIG
                     QIFKTIGFAYLTFVAQRNVALFAIVAAPLLADWANNIIQLIPLSLNSPTGSSALKPAF
                     QKFINGFILIILTLTALGNLYVITQNSRIDSNYPVDGVAWLREHEPQGKLFNSYNWGG
                     YLLWTLPEYPVFIDGRADLYGNEILGQWQDVVNARENAEEILNTWQVNIVFIEPYWDI
                     VPVLKEKGWQVKYEDKSSIILIRP"
     gene            complement(2381..>2653)
                     /locus_tag="PLT08_12790"
     CDS             complement(2381..>2653)
                     /locus_tag="PLT08_12790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE05394.1"
                     /translation="MLPVVEGEEKTRKQILIYTVELIAVTLLLPIFNLAGTIYLISAI
                     VLGAFLFYAAWKVFREGGNKVAWMMYRWSSMYLAFIFLALILDSVL"
BASE COUNT          867 a          536 c          522 g          728 t
ORIGIN      
        1 tttttataaa gcatttcaac tagcattgcc aaaggcaaaa tccatgccag gcgcattgcg
       61 gagtcatgca aaaataaaaa cccaatcatc caagcaaaag aaagcactac gtgccaccat
      121 ttcacttcaa aaagaagaaa aaccaaataa gaataaacgc cataaaacgg agtagctaac
      181 gcagatatca acaagctaga taacatgcgg tctctttttt cgcggaacag aataggtatt
      241 ggcgtcaaaa atattccgaa cttccacaca tctaagcttg ccaagcgcca gacatgaatc
      301 ggtaaatttg agcgcgtttc taatatccac tgtgggatcc aattcacacc aaaaaagata
      361 aaactaagca aaatgatgat ggacggaatc aggaaaagtt taagaaagag gcgcggatct
      421 tcttccatca aaaggcaaaa gaaaattggc aaagccgtca attgaggttt agataaagcg
      481 acaattagac ccaaaccacg caaataagga tttttattaa aaagaaaaac gactagccct
      541 gttgcaatga aaatatctac ttgcccaagc caaacttgac caagcgcagg aaaggaaatc
      601 aataacaaaa acgggttagc ttttgtataa taagcaagta taaggatgcc agatacgcta
      661 aacaaagacc aaacgcccca ggaaaatgga tattctggta accaccgtag tggagcaaga
      721 aaccattgcg caaaatacgg cataaagcca caatgaagac acccttgcgc aaaaggcaga
      781 tagtatcgat ataggtcatc gccgccgggt aaataaaacg aagctgcgat tagcccaatt
      841 gctgcaccaa ttgcggctac ccacattatt ttattagaat cggatgttct caaaacgcct
      901 ccatgaactc agaaaataaa tctctcgaaa aattccttag gtctatttct tttttattga
      961 catttttttt attaagccgg gcacccattg atgcagattt gtggtggcat ttaagggcag
     1021 ggcaattgat gtggcaaaag ggagaaattc tattaaatga cgtctttagc tacaccaagc
     1081 taggttcaaa ttgggtaaat gctttctggg tttctgaggt tttattatat tggatacacc
     1141 ggattggcgg gtatttctta ctaagctttt ttgtttcgct tatcggagct ataacatttc
     1201 tcatcgtatt ctcaagcatg aaagggaact cgctcttaag gagttttatt ctaatattgg
     1261 ctgtgattac ggcagcaccc atttggggac ctcgcccaca aatactttct ttctttctag
     1321 tggcattttt agacttatgg ctaaatcaac catcacggca tcgatggcta ctgataccaa
     1381 tatttgcgct ttgggcaaac attcatggcg gttggatttg gggcttcctg ctccttatag
     1441 ctcatattgc cgggttgttt gtcaattcct tttttgttga ggataagaag aagcttcgta
     1501 cagaagggct gcacttaatt gggtggacag tcctttctgc cttagcggta ggtataaatc
     1561 caaatggcat atatatatgg aaattgccct tccaacaagt aaatgtctca ttacaaatcc
     1621 aagagtggtt atcgcccgat tttcatcgac ttgatttcca ccccatgttg tggatgctct
     1681 ttttattaat tcttttggca ccatttgcgc aaaagccgct acagatcggg caaattttca
     1741 aaaccattgg gtttgcgtat ctcacatttg tcgcgcagag aaacgttgcc ttatttgcaa
     1801 ttgttgcagc accactatta gcagattggg caaataatat tatccagtta atcccgttaa
     1861 gtcttaattc gccaacaggg tcttcagcat taaaaccggc atttcaaaaa tttattaatg
     1921 gatttatatt gatcattctt acattaactg cgctaggaaa tctttacgtc attactcaaa
     1981 atagccgaat agatagcaat taccctgttg atggagtagc ttggttaaga gagcatgaac
     2041 cacaaggcaa actgttcaac tcttataatt ggggtggcta tcttttatgg acattacctg
     2101 aatatcctgt gtttattgat gggcgtgcag atctatacgg aaatgaaata ctaggtcaat
     2161 ggcaagatgt tgttaacgcc agggaaaatg ccgaagaaat attaaatact tggcaggtta
     2221 atatcgtatt tatcgaaccc tactgggata tagtccctgt tttgaaagaa aagggatggc
     2281 aagtaaaata cgaagataaa tcttcaatca tcctcatccg cccataaaag aaaaaggcta
     2341 gccaatttta ttagctagcc tttttagtat caaatacagg ttacaacacc gaatccaaga
     2401 tcaatgccag aaagataaac gccagataca tgcttgacca gcgatacatc atccaagcaa
     2461 ctttatttcc accttctcgg aacactttcc atgcagcata gaagaggaaa gcgcccaaga
     2521 caatagcaga aatgaggtaa atcgttccag ctaaattaaa gataggaagc aaaagagtta
     2581 cagcaatcag ttcaacagta tagattagga tctgcttacg agtcttctct tcgccttcga
     2641 ctacagggag cat
//
LOCUS       DAOQJE010000521         2646 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_27487_length_2646_cov_5.924739, whole genome shotgun sequence.
ACCESSION   DAOQJE010000521 DAOQJE010000000
VERSION     DAOQJE010000521.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2646)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2646)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2646
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27487_length_2646_cov_5.924739"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..680
                     /locus_tag="PLT08_12795"
     CDS             <1..680
                     /locus_tag="PLT08_12795"
                     /inference="COORDINATES: protein motif:HMM:NF013775.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HNE05395.1"
                     /translation="EGYIVTRFVTGKRIMPDEIVKPDYLTRVAQKLRLFHRNAPKLKG
                     EFNVFRRVEMLTKTAKAKGAHFPEDWAFIQKKMREAEKALLKDPYKPTPCHNDLLNLN
                     WLDEEVAGDIGEIRLLDWEYAGMGDIFFDLGNFSHHHRLNEDQIRIFLTAYFGKFTPK
                     HYARLRIMWAMSELHEAMWGTTQSVISTLEEDFQGYANLWFGRYRMHVTDYRWEQWLK
                     DASKKGK"
     gene            795..1670
                     /locus_tag="PLT08_12800"
     CDS             795..1670
                     /locus_tag="PLT08_12800"
                     /inference="COORDINATES: protein motif:HMM:TIGR00167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase family
                     protein"
                     /protein_id="HNE05396.1"
                     /translation="MSIVNAKEIMIPAAKEGWAVGAFNVTDLLQFEAVIDAAIEKKAP
                     VIVQTSVKPSQFLGTNMMVAIYRTLAESAPVPVCLHLDHSTDINYCKKCADAGYTNIM
                     IDASKQSYEENIRQTKEVVDYAHSVGNISVEGELGTVGGVEDQIKVAEDEAQLANPEQ
                     SIEFVERTGVDIFAPAIGTAHGVYKTKNPKIDFERMATIHKMLNSNGIKTPLVVHGGT
                     GLPDDYVAKLLAAGGAKFNVSTELKHTLINAKWEYLSAHRDEYDPGKLDVFVRDATRK
                     AVMHWMDKLGCNGKA"
     gene            1751..>2646
                     /locus_tag="PLT08_12805"
     CDS             1751..>2646
                     /locus_tag="PLT08_12805"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: protein motif:HMM:NF012382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004400 - histidinol-phosphate
                     transaminase activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HNE05397.1"
                     /translation="MAKNIEDYYAPWVKGIPMYISEHIELAWRKPELHRMMSNENPLP
                     PSEKVLEAMFKYAKMTNRYPDQGLVVRQKIAEINGVAGPQNVMIGNGSSEVYDNIFRM
                     FLEPGDEVIQHTPCFGIYGLRGTLLGAKMVSVPMIYKDHKLIFDHEGLMNAVTDKTKI
                     IVIPNPNNPSGNFMDPQHFEPFAKLGIPLVVDEAYIEYAGLGMSQVELTKKYKNVLIT
                     RTLSKAYGLAGMRFGYTIGHEDVIKQIAGSLLPWNVGTIPMWAALAAFEDKEGLAERV
                     KFNNDAVDYISEALSVIPGFYV"
BASE COUNT          704 a          673 c          671 g          598 t
ORIGIN      
        1 ctgaaggcta tatcgtcact cgtttcgtca cgggcaaacg catcatgcca gatgagatcg
       61 tcaagccgga ttatctgact cgtgtggcac aaaaactgcg cctcttccat cgcaacgcgc
      121 cgaaactaaa aggcgagttc aacgtcttcc gccgcgtgga aatgctcacc aaaaccgcca
      181 aagccaaggg cgcgcacttt cctgaagatt gggcgttcat tcaaaagaaa atgcgcgaag
      241 cagaaaaggc attgttgaaa gacccctaca aacccactcc ctgccataac gatttgctca
      301 acctcaactg gttggatgaa gaagtagcgg gtgatattgg cgagatccgt ttgcttgatt
      361 gggagtacgc gggcatgggc gatattttct ttgatctcgg gaatttctcg caccaccatc
      421 gtttgaacga ggatcaaatc cgcatcttcc ttaccgctta ttttgggaag ttcaccccca
      481 agcattatgc gcggctgaga attatgtggg cgatgtctga gttacacgaa gccatgtggg
      541 gcacaaccca atcggtcatc tccacgttgg aagaagattt ccaaggctat gccaatttgt
      601 ggttcgggcg ctatcgcatg catgtcactg actatcgctg ggaacagtgg ttgaaagatg
      661 cgtcgaagaa gggcaagtaa gttagttcat aagtagttgg taattggcga aagaggaaag
      721 aagatagttt attttcattt ttcctctttc gatacaaact cgaaacctga aaagaaaaaa
      781 ccaaggagta ccaaatgtct atcgtaaatg caaaagagat tatgatccct gcggcaaaag
      841 aaggttgggc tgtgggcgcg ttcaacgtca ccgacttgct gcaatttgaa gcggtgatcg
      901 acgcggcaat tgaaaagaaa gcccctgtca ttgtgcagac ttctgtgaag ccttcacagt
      961 tcctcggcac caatatgatg gtagcgatct accgcacatt ggctgaatct gcgcctgtgc
     1021 cggtgtgttt gcacctcgat cacagcaccg atatcaacta ctgcaagaag tgcgcggatg
     1081 ctgggtatac caacatcatg atcgacgctt ccaaacagtc ctacgaagag aacatccgcc
     1141 agaccaaaga agtggtggat tatgctcaca gcgttggcaa catttctgtg gaaggcgaac
     1201 tcggaaccgt tggtggtgtg gaagaccaga tcaaggtcgc ggaagatgaa gcacaactcg
     1261 ccaaccccga acaatccatt gaattcgtcg aacgcacggg tgtggacatt ttcgctcccg
     1321 ccatcggcac cgcacacggc gtgtacaaga ccaagaatcc caagatcgat tttgaacgca
     1381 tggcgaccat tcacaagatg ctgaacagca atggcatcaa gaccccgctc gtcgtccacg
     1441 gcggaaccgg tttgcccgat gattatgttg ccaagttgtt ggcggctggc ggtgcgaagt
     1501 tcaacgtctc caccgagttg aagcacaccc tcatcaacgc caaatgggaa tatctcagcg
     1561 cccaccgtga tgaatacgac ccaggcaagc tcgatgtctt cgtccgcgat gcgacccgca
     1621 aagccgtaat gcactggatg gacaagctcg gctgtaacgg caaggcttaa tcataagaca
     1681 atcaacgatg tccggcagat aagatcgtcc atcgtgataa ttcgtaaaat aatttaggag
     1741 aacaagtaac atggctaaaa atatcgaaga ttattacgcc ccgtgggtga agggcattcc
     1801 gatgtacatt tcggagcaca ttgagttggc ttggcgcaag cccgaactgc atcgcatgat
     1861 gtccaatgaa aatccgctgc ccccttctga aaaggtgcta gaggcgatgt tcaagtacgc
     1921 caagatgacc aatcgctatc ccgatcaggg tttagtggtg cgtcagaaaa ttgctgagat
     1981 caatggcgtt gccggtccgc agaacgtgat gattggcaac ggctccagcg aagtgtatga
     2041 caacatcttc cgcatgttcc tcgaacccgg cgatgaagtc attcagcaca ccccctgctt
     2101 cggcatttac ggtctgcgcg gaaccttgct cggcgccaag atggtctctg tgccgatgat
     2161 ctataaagat cataagctca tcttcgacca tgaaggtctg atgaacgcag tcactgataa
     2221 gaccaagatc atcgttatcc cgaaccccaa caaccccagc ggcaacttca tggacccgca
     2281 gcacttcgaa ccgtttgcca agttgggcat tccgttggtt gttgatgaag cctacattga
     2341 atatgccggc ttgggcatgt ctcaggtcga attgaccaag aagtacaaga acgtgctcat
     2401 cacccgcaca ctctccaaag cctatggctt ggcgggtatg cgcttcgggt acaccatcgg
     2461 tcacgaagat gtgatcaagc agatcgccgg ctccctcctg ccgtggaatg tgggtaccat
     2521 ccccatgtgg gctgcgcttg ccgcttttga agacaaagaa ggtctggcag aacgcgtcaa
     2581 gttcaacaat gatgcggtag attacatcag cgaagctttg agtgtcatcc ccggcttcta
     2641 cgtgat
//
LOCUS       DAOQJE010000522         2644 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_27528_length_2644_cov_3.926226, whole genome shotgun sequence.
ACCESSION   DAOQJE010000522 DAOQJE010000000
VERSION     DAOQJE010000522.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2644)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2644)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2644
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27528_length_2644_cov_3.926226"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..299
                     /locus_tag="PLT08_12810"
     CDS             <1..299
                     /locus_tag="PLT08_12810"
                     /inference="COORDINATES: protein motif:HMM:NF025442.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF4258 domain-containing protein"
                     /protein_id="HNE05398.1"
                     /translation="PSRHCIITAMNSILFRAHAVQRMFEREVSVTKVREALKTADVIE
                     DYSFETPEPSCLLFGQQGRRPFHVVTSQSQQHITVITVYLPSPEKWKKDLRSRR"
     gene            296..517
                     /locus_tag="PLT08_12815"
     CDS             296..517
                     /locus_tag="PLT08_12815"
                     /inference="COORDINATES: protein motif:HMM:TIGR03831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgiT-type zinc finger protein"
                     /protein_id="HNE05399.1"
                     /translation="MICTVCREAHTTHSLTSLQFERDEFRATVHHIPAQVCPACGESY
                     LEEEVTAKLLDRVQQSMAEGELDIVLDYL"
     gene            514..1665
                     /locus_tag="PLT08_12820"
     CDS             514..1665
                     /locus_tag="PLT08_12820"
                     /inference="COORDINATES: protein motif:HMM:NF014215.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CocE/NonD family hydrolase"
                     /protein_id="HNE05400.1"
                     /translation="MKKWFPWLWVLLTACTSAPLTPTATLPPPVVTIIPATQTPTPLP
                     TLTPTATPLPYLMYPYTIAGLREKDFPGGEILIGARVAFNDQFSTYLISYPSEGLTIT
                     GVLNLPAGQGPFPVIVMNHGFFPRTQYSSGDGSQRAAEYLAQRGYLTISSDYRTWGGS
                     DFGVSYFHTGLVTDVMNLLASLHSLPQADTSRIGMWGHSMGGGITTKILTIQGGRVAL
                     SDNEGRIAKSLEGETTVRAAVLYAPNSADDADLIARWGYGCIGDIAAGELLNTCNSAD
                     VIPETLPAEVIAAYKETAQSPERMREVAPYYHLDWVQVPVQIHIGSADGDYIGSTPPE
                     WSYKLNQGLLDAGKEVELFVYQNQRHSFTGDAWILFMDRAIAFFDENLK"
     gene            1662..>2644
                     /locus_tag="PLT08_12825"
     CDS             1662..>2644
                     /locus_tag="PLT08_12825"
                     /inference="COORDINATES: protein motif:HMM:NF014215.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HNE05401.1"
                     /translation="MKPSYKIICLLLLWILSACASNPTPPTAQSLATTSPPTSTPTET
                     PLPIPTATLTTDQALAPYTIAGLRARDYEKGKITLGEPVLETEHFTRYLIKYPSDGLT
                     ITGVLQIPKVGEAPYPVIVMNHGFFSRYVYYSGDGTDRAADFLNRRGYLTVASDYRSW
                     GESDTAESLFYSGQVIDVINLMNALESIPEADTSRMGMWGHSMGGGITAKVLTIVDDR
                     IKAAVLYSSVSADFADVIGRWGPGCVGDVLIGEATFGCNSSDILPLNLPAEVTASYFD
                     ATSDPVMLQAVSPLYHFDLVQAPVHIAYGTEDGKTSAGTPPEWSKKMYAAFT"
BASE COUNT          594 a          884 c          592 g          574 t
ORIGIN      
        1 caccttcccg gcattgtata atcaccgcca tgaactccat cctattccgc gcccatgccg
       61 tacaacgcat gttcgaacgg gaagtatccg tcacgaaagt tcgcgaagca ttaaaaacag
      121 cagacgtgat cgaagactat tcctttgaaa cccccgaacc aagctgctta ttgttcggtc
      181 agcaaggcag gcgacccttc catgtcgtca cctctcaaag ccagcaacac atcaccgtca
      241 taaccgtcta cctgccttca ccagagaaat ggaaaaaaga cctgcggagc cgccgatgat
      301 ttgcactgtc tgtcgcgaag cacacacaac ccacagccta acctcgctcc aattcgagcg
      361 tgacgagttt cgggcaaccg ttcaccacat ccctgcccaa gtttgccctg cctgcggcga
      421 gtcctatcta gaagaagagg tcacagccaa actgttagat cgtgtgcagc agagcatggc
      481 agaaggtgaa ttggatatcg ttctggacta cctatgaaaa aatggttccc ttggttatgg
      541 gtactcctaa ctgcttgcac atctgcgcct cttaccccca ccgccacctt acccccgcca
      601 gtagtcacca tcatccccgc cacccaaacg ccaaccccac tgcccaccct cacgcccaca
      661 gccacccctc tgccatacct catgtatccc tacaccatcg ccggtctgcg tgaaaaagac
      721 ttccccggcg gcgagatact aatcggcgcg cgcgtcgcct tcaacgacca attcagcacc
      781 tatctcattt catatccctc cgaagggctc accatcaccg gcgtgctcaa cctcccagcc
      841 gggcagggac ccttcccggt catcgtcatg aaccacggct tcttcccccg cacacaatac
      901 tcctctggcg atggctctca acgtgccgcc gaatatctcg cccagcgcgg ctacctcacc
      961 atctcatcag actatcgcac ctggggcggc tccgattttg gcgtctccta cttccacacc
     1021 ggcttggtca ccgatgtgat gaacctgctc gcatcgctcc actcccttcc ccaagccgat
     1081 acctcccgca tcgggatgtg gggtcacagc atgggcggtg gcatcaccac caagatcctc
     1141 actatccaag gtggtcgagt agccctgagt gataacgaag ggcgtatcgc gaagtccctg
     1201 gaaggggaga ccaccgtccg cgccgccgtg ctttacgccc ccaactccgc cgacgatgcc
     1261 gacctgattg cgcgctgggg ctacggctgc attggcgata tcgccgctgg tgaattgctc
     1321 aatacctgca actctgccga tgtcatccca gagacacttc cagccgaggt cattgccgcg
     1381 tataaagaga cagcccaaag tccagaacgg atgcgcgaag tcgcacccta ctaccattta
     1441 gactgggtcc aagtccccgt acagattcac atcggttcag ccgacggcga ttacatcggc
     1501 tccacgccgc ccgaatggtc gtataaactt aatcaaggct tgctcgatgc aggcaaagaa
     1561 gtcgagttgt ttgtgtacca aaaccaacgt cactccttca caggcgacgc ttggatttta
     1621 ttcatggatc gtgctattgc ctttttcgat gagaacttaa aatgaaacct tcgtacaaaa
     1681 taatctgtct tctgctgtta tggatactgt ctgcttgcgc atccaaccca accccgccca
     1741 cggcacaatc gcttgccaca acctctccgc ccacctccac cccaaccgag actccgcttc
     1801 ccatcccaac tgccaccctc accaccgatc aagccctcgc gccctatact attgcagggc
     1861 tgcgcgcccg tgactatgaa aaaggcaaga tcacattagg cgaaccggtt ttagaaaccg
     1921 agcacttcac tcgttatctc atcaaatacc caagcgacgg gttgaccatc accggtgttt
     1981 tgcaaattcc aaaagtgggc gaagcgccct atcccgtcat cgtgatgaat catggcttct
     2041 tctcgcgcta tgtttactac tctggtgatg gcaccgaccg cgccgccgac tttctcaatc
     2101 gccgcggcta tctcaccgtc gcgtcagatt atcgtagctg gggcgagtct gataccgccg
     2161 agagtctttt ctactcgggt caagtcatcg acgtgatcaa cttgatgaac gcactggaat
     2221 ccattcccga agccgatacc tctcgcatgg gcatgtgggg gcatagcatg ggcggcggca
     2281 tcacagccaa agtcctcacc attgtcgatg atcgcatcaa agccgccgtt ttatattcat
     2341 ccgtcagcgc cgactttgcc gatgtgatcg ggcgttgggg tcccggctgt gtgggcgatg
     2401 tcttgatcgg cgaagcgacc ttcggctgca attcatccga cattcttcca ttgaacctgc
     2461 cagccgaggt caccgcctcc tacttcgacg ccacgtctga tcctgtgatg ctgcaagcgg
     2521 tttctccgct ctatcatttt gacttggtcc aagcccctgt ccacatcgcc tacggcaccg
     2581 aagacggcaa aacctccgcc ggcaccccgc ccgaatggtc aaagaaaatg tatgccgcct
     2641 ttac
//
LOCUS       DAOQJE010000523         2639 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_27618_length_2639_cov_7.079721, whole genome shotgun sequence.
ACCESSION   DAOQJE010000523 DAOQJE010000000
VERSION     DAOQJE010000523.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2639)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2639)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2639
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27618_length_2639_cov_7.079721"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(113..1606)
                     /locus_tag="PLT08_12830"
     CDS             complement(113..1606)
                     /locus_tag="PLT08_12830"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNE05402.1"
                     /translation="MKVELKNIHKHFGKTYANKGINLNIPSGSIQGILGENGAGKSTL
                     MKILSGFIQADPGGEIVLDGKPAVMQSPADAIRFGVGMLHQDPLDFPPMKLIDNFLLG
                     MSGKLFPNRKETVQDFNLLAKEYGFNFDPENYVDSLTVGERQQLEIMRLLFLGARVFI
                     LDEPTTGISAAQKIKLFDTLRTFPEQAITTIFVSHKLEDVESLCDRVAVLRAGELVGE
                     FTPPYETKKFVSTMFGKEITLGERQPAKTGDVVLSLEDVSLEDSRVRIDHVNVDIRAG
                     EVIGLAGLEGSGQGMFIRACAGLVRPVGGKVMLKGKNLTGKPYHTFKHEGVNFLPAAR
                     LEEGLVPGLSLTDHFVLAEEPKGVFIDKAYGQKLAEDRIKSFNIRGTPASMIESLSGG
                     NQQRAELALLQTPLSLVLLEHPTRGLDIESVIYIWSKLKDRCKQEGAAIIFISSDLEE
                     VLQYSDRLLVFFSGKVSEPIDAATTSVEQLGQLIGGKGWTKKGDIHA"
     gene            complement(1683..>2639)
                     /locus_tag="PLT08_12835"
     CDS             complement(1683..>2639)
                     /locus_tag="PLT08_12835"
                     /inference="COORDINATES: protein motif:HMM:NF014647.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HNE05403.1"
                     /translation="TTPDQLAEELVAQGATLIIFNSDDMKDASTTFAKNHPDVYVIMA
                     SGDQVWSDGKAFEAMPNLTNIMGRMEYGKMMAGCAAALASETGKIGYLGPLINDETRR
                     LAASAYLGAKYCYEKAGNDPASLEFKVTWIGFWFNIPGVTLDPVQVSNDFYTTDYDVV
                     ISGIDNPVNLGEAAKLKAEGKNVSGVAYDYVAACEAAPDVCIGVPYFNWGPEYVKAIS
                     SAQDGSWQSQFIWAGPDWADINNPDTSTVGFVKGPALADDDAATLDAFIAELAGGLNL
                     WSGPINLQDGSAYLADGEVATDQQIWYLPQLLEGMDGLSAAE"
BASE COUNT          692 a          686 c          624 g          637 t
ORIGIN      
        1 atttttatac gcttccatag gagaagcgcc tactgccaac agaattaatg agacaaatcc
       61 catagcaagc aggaacgcga ttaattgaaa aataaaccca cccgctttgg tcttaagcat
      121 ggatgtctcc ttttttcgtc caacccttac cgccgatcaa ttggcctagt tgttccacac
      181 tggttgtcgc agcatcgatc ggttctgaaa ccttaccgct aaagaacact agcaaacggt
      241 cactgtactg taaaacttct tccaagtcag acgaaataaa gatgatggct gcgccctctt
      301 gtttgcagcg gtctttgagt ttgctccaaa tatagatcac cgactcaata tccaagccac
      361 gagttgggtg ctccaataag accagcgata gtggcgtctg taaaagcgcc aattctgccc
      421 gctgttgatt tccacccgaa agcgactcga tcatactggc aggcgtaccg cgaatgttga
      481 aggatttgat gcgatcttcc gccagttttt gaccatatgc cttatcaatg aacacgccct
      541 tcggctcttc cgcaaggacg aagtggtcgg tcaggctcaa gccgggcact aagccttctt
      601 ccaatcttgc ggcgggaagg aagttgacac cttcatgctt gaaagtgtga tacggcttcc
      661 ccgtcaaatt cttaccttta agcataacct taccacccac cgggcggacg agcccagcac
      721 atgcacgaat aaacatgcct tgcccagaac cttccaaacc agcaagacca atcacttctc
      781 cagcacgaat atcgacattc acatgatcga tgcgaacacg ggaatcttcc aaagagacat
      841 cttccaacga aagcacaaca tcgcccgttt tggcaggttg acgttcacct aaagtaatct
      901 ctttgccaaa catggttgaa acaaatttct tggtttcata aggtggcgta aattcgccaa
      961 ccagttcccc tgctcgcaac acggcgacac ggtcgcacag gctttcaaca tcttccaatt
     1021 tatgtgacac aaaaattgtt gtaatggctt gttctggaaa tgttcgaagc gtatcaaaca
     1081 attttatctt ttgcgcagca ctaattcccg tcgtcggctc atccaaaatg aacacacgcg
     1141 caccaaggaa taataaacgc atgatctcca actgttggcg ttcaccaacc gttagagaat
     1201 ctacgtaatt ttcggggtca aaattaaaac catattcttt tgccagcagg ttaaagtcct
     1261 gtacggtttc ttttctattc gggaaaagtt ttcccgacat gccaagcaaa aagttatcga
     1321 tcaatttcat gggaggaaaa tcgagcgggt cctgatgcaa catccccacc ccaaatcgga
     1381 tggcatctgc tggagattgc atcactgcag gttttccgtc taaaacgatc tcaccacctg
     1441 ggtccgcttg aatgaacccg gataagatct tcatcagggt gcttttccct gcgccgttct
     1501 cccccaaaat accctgtatc gagccagaag gaatattaag attaattccc ttattggcat
     1561 aggtcttgcc gaagtgcttg tgaatgttct tgagttcgac tttcatggtg gtgcgggtcc
     1621 tccatgaggg tgaaatgaaa atgtaggggc gcggtgtccg cgcccctaca aagggaacta
     1681 cattattcgg ctgcgctcaa gccatccata ccttcgagca attgcggcag gtaccagatc
     1741 tgctggtctg tagcaacttc accgtcggcg aggtaggcgc ttccgtcttg caggttgatc
     1801 ggaccagacc aaaggttcag tccgccagcg agttcggcaa tgaatgcatc gagggtagca
     1861 gcatcatcgt cagccaaggc aggtcccttc acgaaaccaa ccgtggaggt gtctgggttg
     1921 ttgatgtcag cccaatcggg tccagcccaa atgaactgag actgccagga accatcctga
     1981 gcggaagaga ttgccttcac gtattcagga ccccagttga aatacggaac accgatacaa
     2041 acatcaggag cagcttcaca agcggcaaca taatcgtatg ccacaccaga gacattcttg
     2101 ccttctgcct tgagttttgc agcttcacca aggttgacag ggttgtcgat accggagatg
     2161 acaacatcgt aatcggtggt gtagaaatcg ttagacacct gaacggggtc gagggtcacg
     2221 ccggggatgt tgaaccagaa accgatccag gtcaccttga attccaagga agcaggatca
     2281 ttgccagcct tctcatagca gtatttcgcg ccgaggtagg cagaggcagc gaggcggcgg
     2341 gtttcatcgt tgattagcgg tccgaggtaa ccgatcttac cggtctcaga agcgagagca
     2401 gcggcacaac cagccatcat cttgccgtat tccatgcgtc ccatgatatt ggtgaggttg
     2461 ggcatggctt cgaacgcctt gccatcagac caaacctgat caccagaagc catgatgacg
     2521 tacacatcag ggtgattctt ggcgaaggtg gtggaggcat ccttcatatc atcagagttg
     2581 aagataatga gggtagcgcc ctgtgccacc aattcttcag cgagctggtc gggggtggt
//
LOCUS       DAOQJE010000524         2638 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_27641_length_2638_cov_5.987224, whole genome shotgun sequence.
ACCESSION   DAOQJE010000524 DAOQJE010000000
VERSION     DAOQJE010000524.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2638)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2638)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2638
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27641_length_2638_cov_5.987224"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1155
                     /locus_tag="PLT08_12840"
     CDS             <1..1155
                     /locus_tag="PLT08_12840"
                     /inference="COORDINATES: protein motif:HMM:NF024883.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HNE05404.1"
                     /translation="YLVNANFAVLTNLRFQETILGKTYIRTDGVTSAIEQQGNGQASY
                     NDYRDVPVLGVYHWIPELKAAVLAEHDQSEALQTSSNLFLVNLIVAILTIGLASIAAF
                     ILTRQITTPITELANAAEGISRGNLDNEVKINRGDEIGLLASSFNLMTSRLRDLVNTL
                     EQRVADRTKALTASAEVSRRISTILDQKQLVIEVVEQVKNAFNYYHAHIYLFNETGDS
                     LIMVGGTGEAGQIMLDRGHKIPKGRGLVGRAGETNTPVLVSDTSTDPDWLPNPLLPDT
                     KSELAVPIAINDNVLGVLDVQQNIVGGLKQEDSDLLQSIANQVAVAIQNTRSYSQAQQ
                     RAEREALISSINQKITSETSVERALQVAVREVGRALNTQAKVKINTTGKG"
     assembly_gap    1269..1368
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1369..>2638
                     /locus_tag="PLT08_12845"
     CDS             <1369..>2638
                     /locus_tag="PLT08_12845"
                     /inference="COORDINATES: protein motif:HMM:NF024583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HNE05405.1"
                     /translation="YASNAELDEGEYSIYFSAPIFDDNNKPIGIVRIRYDANVLQDIL
                     LKTAKNSNIEGLVIDLFDENHVALAATDAPEDILKILVLPSDEKLKELQEAGRILEGS
                     AEEVALNDPDLETHLRNAEQTPFFTIESEGEEAATAFIQSKEWIVLAGQDQSIYQAPL
                     QEQLRIEITIGLALLVIVSALALLMARSISRPIIDLSGVAKDIANGNIDLQAKVQTTD
                     EIGDLATSFNDMTSRLRSSFIDLERRAKEITTISEVSRRLSSVLDKQRLLIEVVEQIK
                     SSLDYYHVHIYLLDKDSGDLIMSGGTGEVGQTMLERKHRISKGRGLVGRAAESGAPVL
                     VPDTLKDASWLPNPLLPETASEIAVPILSETGVVGVLDVQNNIQNSLSERDVELLQSI
                     AYQVSIALKNADTYMEAQSQAERENIINEIN"
BASE COUNT          745 a          552 c          580 g          661 t
ORIGIN      
        1 tatttggtta atgccaattt tgctgtattg accaacttac gtttccaaga aaccatcttg
       61 ggcaagacct atatccgcac agatggggtt acaagtgcta ttgaacaaca aggaaacggt
      121 caagctagtt acaatgacta tagagatgtg cctgttcttg gggtatatca ttggatccct
      181 gaactgaagg ctgccgttct tgctgaacac gatcaatctg aggcgctgca aacatccagt
      241 aatttgttct tggtcaacct aatcgtagct attcttacaa ttggtcttgc aagtatcgct
      301 gcttttattc ttacccgtca gatcacaacc cccattaccg agcttgccaa tgcagccgaa
      361 ggaatctcac gcggtaattt ggacaatgaa gttaagatca atcgtggaga tgaaattggc
      421 ttgttggcga gcagtttcaa cctgatgacc tcgcgcttaa gagacttggt caatacactg
      481 gaacagcgtg tagcagaccg caccaaggcg ttgacagcct ctgcagaagt cagccgccgt
      541 atttcaacta ttcttgacca aaaacaattg gtcatcgaag ttgtagaaca ggttaagaac
      601 gctttcaatt attaccacgc tcatatttac ttgttcaacg aaacgggaga tagtctaatc
      661 atggtgggtg gtacaggtga agcggggcaa atcatgttag accgtggaca caaaattcca
      721 aagggcagag gcttggtggg tcgtgccggc gaaaccaata ccccggtttt ggtttctgat
      781 acatccacag acccagattg gctacccaat cctttattgc ccgatacaaa atctgagttg
      841 gctgtgccca tcgccattaa tgacaatgtt ttaggggtgt tggatgtaca acaaaatatt
      901 gtaggtggat taaagcagga agattcagac ttgcttcaat cgattgcgaa tcaggttgca
      961 gttgccatcc aaaatacacg gtcgtattca caagcccaac aacgcgcgga gcgtgaggcg
     1021 ttgatctctt ccatcaatca aaagattacc agcgagacct ctgtcgaacg cgcactgcaa
     1081 gtagcagtcc gtgaggtcgg gcgcgctttg aatacccagg cgaaagtgaa aatcaatacc
     1141 acaggtaaag gataaaagaa aatgaaacca ttaaaaatat ccctatctgt aaaattagtg
     1201 tttgctttct tgttcttgtc actcatccca ttgggtatcg ttatctactt caatgcagtt
     1261 tttggtagnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1321 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnta cgcctctaat
     1381 gccgagcttg atgaaggcga gtattcgatc tattttagcg ccccgatttt cgatgataat
     1441 aacaagccta ttggcattgt tcgaatccgc tatgatgcga atgtcttaca agatatctta
     1501 cttaaaacag caaaaaactc aaatatagaa ggtcttgtca ttgacctgtt cgatgaaaat
     1561 catgtggcat tggctgcaac ggatgcgcct gaagatattc tcaaaatact tgttcttcct
     1621 tctgatgaaa agctgaaaga actgcaggaa gctgggcgta tattggaagg ttctgcagaa
     1681 gaagtggcat taaatgaccc agatctagag acgcacttaa gaaacgcaga acaaactccc
     1741 ttttttacga ttgaatcaga gggagaagaa gctgcaactg cttttattca aagcaaagaa
     1801 tggattgttc tagccgggca ggatcaatca atttaccaag ccccgcttca agagcaacta
     1861 cgtattgaga tcactattgg tcttgcattg ctagtcatcg tttcagccct tgcactattg
     1921 atggcaagaa gcattagccg ccccattatt gacctttctg gggtagcaaa agatattgca
     1981 aatgggaata tagatttaca agcaaaagtc caaacaaccg atgaaattgg agatctggca
     2041 accagcttca atgatatgac ctcaaggtta agatcgagtt tcattgattt ggaacgtcgc
     2101 gccaaggaaa ttactaccat ttccgaagtg agccgccggt tatcttccgt gttggataag
     2161 caacgcctct taatagaggt cgtagaacag atcaaatcct ccctggatta ctaccatgtc
     2221 cacatttacc ttttggataa ggacagcggc gacttgatca tgtctggcgg aaccggggaa
     2281 gttgggcaaa ctatgttgga acgaaaacat aggatcagca aagggcgcgg cttggtcggg
     2341 cgtgccgcag aatcaggcgc ccctgttctg gtgccagaca cgctaaaaga tgcaagctgg
     2401 ttacctaacc cgcttttgcc agaaaccgct tctgaaattg cggttccaat cctttcagaa
     2461 actggagtgg ttggggtttt ggatgtacaa aataatatcc agaacagtct atccgaaaga
     2521 gatgtggaat tgttgcagtc cattgcttac caagtatcga tcgccttgaa aaatgcagac
     2581 acgtatatgg aagcccaaag ccaggcagag cgcgaaaaca tcatcaacga aatcaacc
//
LOCUS       DAOQJE010000525         2629 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_27801_length_2629_cov_6.044678, whole genome shotgun sequence.
ACCESSION   DAOQJE010000525 DAOQJE010000000
VERSION     DAOQJE010000525.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2629)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2629)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2629
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27801_length_2629_cov_6.044678"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(128..1282)
                     /locus_tag="PLT08_12850"
     CDS             complement(128..1282)
                     /locus_tag="PLT08_12850"
                     /inference="COORDINATES: protein motif:HMM:NF016764.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0031267 - small GTPase binding [Evidence
                     IEA];
                     GO_process: GO:0006888 - endoplasmic reticulum to Golgi
                     vesicle-mediated transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Yip1 family protein"
                     /protein_id="HNE05406.1"
                     /translation="MTDLNQAIQLIRQGQKFEAQRILQEIIKTEPKNIPAWFWYVETC
                     ATTERRIQVLETCLKMNPGNLQVEQALQTFRNSQPPVPAPPKVEPPAYQSNTPEPQKN
                     DDLYGYTSFRSDSFEQTQSYEEDKSPAYDSPLYEYETFRTNSSVKAETQKKQPWEMDL
                     STYEDNSMLAKSKKVRRSYSVFDVWLTALTSQDEKSYADLLEDPEAGLERAFSWVAIA
                     GLITALTVPVQYFLNPNLGELLDIPELQRQVGNMTPNAFIIILTMISFVTAPIGAILN
                     LALSGGIQNLLARFFGGGGNFKRTAYALSAFLAPMAVVTSLVAIVPVAGGCLSFPLGI
                     YSFILNVRALKASHSLTNGAAIGVLLAPSILVIIFGCLFLFMSGTSLSSS"
     gene            complement(1354..>2629)
                     /locus_tag="PLT08_12855"
     CDS             complement(1354..>2629)
                     /locus_tag="PLT08_12855"
                     /inference="COORDINATES: protein motif:HMM:NF013481.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016625 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, iron-sulfur
                     protein as acceptor [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aldehyde ferredoxin oxidoreductase C-terminal
                     domain-containing protein"
                     /protein_id="HNE05407.1"
                     /translation="GVGAVMGSKNLKAVVAMGNKNPPLHDSEGMRAISVETSKNVGAD
                     VKKGSNMRIYGTSYVPQVTNTLGILPTRNFLQGTFEDVHNVDGDALKNQYLIRHTPCY
                     RCPLSCGRLTEVPDGPYKGKGEGPEYETISSLGTGCGVSNLAALVKANYLCNEYGMDT
                     ITTGMTIAVAMEMYEKGYLTEETIGMPLKFGDHDAMIEMIKLMAYNKGFGQDLVQGSY
                     RLAEKYGHPELAVTTRKQEFPGYDPRGSQGMGLLYATSNKGASHMEGDVAYEEVFGVP
                     IKENPLSTEGKPELVKHFQDSFALIDSSGLCVFVAIRYVFDKNRLIWPQTLAAMMNLS
                     TGAGYTPEEVMKAADRVYTLERMFLIKAGSGPEWDVLPYRMLHEPLPDGPAKGKVAEL
                     DKMLPEFYEKRGWDEKGYPTQAKLMELGLPLN"
BASE COUNT          715 a          621 c          608 g          685 t
ORIGIN      
        1 tttacagaga aggtttgctg gagagtcggt catgtatgga tgataacaaa ggtacaagaa
       61 actgaatcaa ctatccctta agaaagaagc gggtgtattt ggaagcatcc gcttcttttt
      121 ggatatctta agaggacgat aatgaagttc ctgacataaa aagaaataaa cacccaaata
      181 taataactag aatagatggc gctaataaga cgccgattgc agcaccgttt gtcaaagagt
      241 gagaagcctt caaggcgcgg acgttcagaa taaaactata aatgccgagc ggaaaagaaa
      301 ggcacccccc cgcaactggc acaattgcca caagggaagt aaccactgcc attggtgcaa
      361 ggaatgcaga taatgcatac gctgttcgtt taaagttgcc gccaccaccg aagaaccgcg
      421 ccaacaaatt ttggatgccc cccgaaagtg caagattaag aatcgctcca ataggcgccg
      481 tgacaaaaga gatcatggtc agaataataa tgaaggcatt gggcgtcata ttcccaactt
      541 gccgttgcaa ttcgggaata tcaagaagct ctcctaaatt agggttgaga aagtattgga
      601 caggaactgt caaagccgtt attaacccgg caatggcgac ccaactaaaa gcacgttcaa
      661 gacctgcttc tgggtcttcc aataggtcgg catatgattt ttcatcctga gaggttaaag
      721 ctgtcaacca tacatcaaac acagaataac tacgccgaac ctttttagac ttggctaaca
      781 tggagttatc ttcgtaggta gacaaatcca tttcccaagg ttgcttcttc tgagtctctg
      841 ccttcactga tgaatttgtg cgaaatgttt cgtactcata caagggcgaa tcatatgctg
      901 gagatttatc ttcttcataa gattgtgtct gctcaaaaga atcggaacgg aatgaggtgt
      961 aaccgtacaa atcgtcgttc ttttgaggtt caggagtgtt gctttgataa gcgggcggtt
     1021 cgaccttggg aggggctggt acgggcggtt gggagtttcg aaaagtctgt aacgcctgct
     1081 ctacttgaag gttgccaggg ttcattttta ggcaagtttc aagaacttga atccgtcttt
     1141 cagtggtcgc gcaggtttcc acataccaaa accaagcagg gatgttcttc ggctcggtct
     1201 tgataatttc ttgcagaatg cgctgcgcct caaatttttg cccctgacga atgagttgga
     1261 tggcttgatt tagatctgtc attcgaactc ccaagaaaaa gatatgccca tagcttgtgt
     1321 tgaccaagct atgggcaggt taaaacaact ctgctaattt agcggcaaac ccaattccat
     1381 taactttgcc tgggtggggt aacctttttc atcccagcca cgtttttcat agaactctgg
     1441 caacatcttg tcgagttctg ctacttttcc cttggcagga ccatcaggca aaggttcatg
     1501 caacatgcgg taagggagca catcccattc gggaccagac cctgctttga tgaggaacat
     1561 gcgctcgaga gtataaacac ggtcggcggc tttcatcact tcttcagggg tataacctgc
     1621 acctgtggat aaattcatca tagctgccaa ggtctgaggc cagataaggc ggttcttgtc
     1681 gaagacatag cgaatggcaa cgaatacgca caaccctgag gagtcgatca aggcaaacga
     1741 atcttggaag tgtttgacca gttctggttt tccttccgtt gaaagcggat tttccttgat
     1801 cggcacacca aagacttctt catacgccac atcgccttcc atatgtgacg cgcccttgtt
     1861 ggaggtggca tacaacaagc ccataccttg cgagccacgt ggatcatagc cggggaattc
     1921 ttgcttacga gtcgtcactg ccaactcggg gtgaccgtac ttttccgcaa ggcgataact
     1981 accttgcacc agatcctgac caaagccttt gttataagcc atcagcttga tcatctcgat
     2041 catggcatca tggtcgccga acttaagcgg cataccaata gtttcttcgg tgagatagcc
     2101 cttttcgtac atttccattg ccacggcaat tgtcatgccg gtggtgatgg tatccatacc
     2161 gtattcattg cacagataat ttgcttttac caatgcagca agattgctca caccgcaccc
     2221 cgtacctaaa gatgaaatgg tttcatattc aggaccttct cccttgcctt tatacggacc
     2281 atcaggcact tcggtgaggc gcccacatga aagcggacag cgataacaag gggtatggcg
     2341 aatcaaatat tgattcttca aggcatcgcc atctacattg tgaacatctt caaaagtgcc
     2401 ttgtaaaaag tttcgcgtgg gcaaaatgcc gagggtgttg gtcacttgtg gcacatacga
     2461 agtgccataa atacgcatgt tcgagccctt ctttacgtcg gcaccaacat tcttggaggt
     2521 ctccacgctg atggcacgca tcccttcaga atcatgcaga ggcgggtttt tatttcccat
     2581 tgccactacg gctttgagat tcttacttcc catcacagcg cccacaccc
//
LOCUS       DAOQJE010000526         2628 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_27817_length_2628_cov_6.004664, whole genome shotgun sequence.
ACCESSION   DAOQJE010000526 DAOQJE010000000
VERSION     DAOQJE010000526.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2628)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2628)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2628
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27817_length_2628_cov_6.004664"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..532)
                     /locus_tag="PLT08_12860"
     CDS             complement(<1..532)
                     /locus_tag="PLT08_12860"
                     /inference="COORDINATES: protein motif:HMM:NF016413.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LURP-one-related family protein"
                     /protein_id="HNE05408.1"
                     /translation="MSTRYKIRQNLISIGDDFWIENEEGKRVFKVDGKVLRIRKTLVF
                     EDANGKKLAQIQERLLTIKDTMVVEDADGKDLATIKKALLTPFRDRWDVKVKNGPDLD
                     VQGDFLAHEYTIKAGRNKVAEVSKKWLRITDTYGVEIEEGQNDILILAIAVAIDIMVD
                     DEAKKKKEKKEKLEKKK"
     gene            complement(623..961)
                     /locus_tag="PLT08_12865"
     CDS             complement(623..961)
                     /locus_tag="PLT08_12865"
                     /inference="COORDINATES: protein motif:HMM:NF022279.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YrdB family protein"
                     /protein_id="HNE05409.1"
                     /translation="MDALKQLNLALRFLLELCMLAAVGFWGFKTHQTWFPKILFGIGF
                     PLLIAIVWGTFLAPRATRPLTGASFLTLELILFSSGAIALFASGKPTLGWVYTIALVV
                     NKVLLIVWKQ"
     gene            complement(975..1550)
                     /locus_tag="PLT08_12870"
     CDS             complement(975..1550)
                     /locus_tag="PLT08_12870"
                     /inference="COORDINATES: protein motif:HMM:NF024701.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WHG domain-containing protein"
                     /protein_id="HNE05410.1"
                     /translation="MPRKTRTRLDKNAVVQAAVEILNAEGVQALTLSRLAEELGIQTP
                     SLYNHVEGLPGLQQELAILNAKLLADRLSSAAMGKSGTELFMEASQAFRAYVKEYPGL
                     YMSTLRSSGAQETPNKELQLEEERTVNVGLVVMASLGLKGENAIHGLRAFRSMVHGFA
                     TLEVAGGFGLPQDCDESFRRLVLALVAGLQK"
     gene            complement(1553..2353)
                     /locus_tag="PLT08_12875"
     CDS             complement(1553..2353)
                     /locus_tag="PLT08_12875"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HNE05411.1"
                     /translation="METRFFNYKTGQIAYEVQGEGPLVVCVPSLGDVRGEYRFVVPEL
                     VKAGYRVATMDVRGHGETSTTWDDFSVAAIGEDILALIRELKAESAIVVGTSMGGGAA
                     IWSAVEAPELIRGMLLIDPFVDGDSNPFFIFLLSIMFARPWGAAMWKKYYSSLYPTRK
                     PADFDLYVETLYKNINQLGRLEAVMAMMRASKRSSGERMPNVKQPALILMGSKDPDFK
                     NPIAEAKRVAEAVRGDYKIIENAGHYPHAEMPEVTIPLMLDFMNSLKG"
BASE COUNT          677 a          639 c          574 g          738 t
ORIGIN      
        1 actttttctt ttcgagcttt tctttctttt cttttttctt cttggcttcg tcatctacca
       61 tgatgtcaat ggcgacggcg atggctaaga tcaaaatatc gttctgcccc tcttctattt
      121 ctacgccata agtatcggtg atgcgcaacc attttttgga gacttcggcg accttattgc
      181 gacccgcttt gatggtgtac tcatgcgcga ggaagtcacc ttgcacatcg aggtcgggcc
      241 cattcttcac tttcacatcc caacgatcac ggaagggtgt gagaagcgct ttcttgatgg
      301 tggcaaggtc tttgccatcg gcgtcttcta ccaccatggt gtctttgata gtgagcaaac
      361 gttcctgaat ctgagcgagt ttcttgccgt ttgcatcttc aaagaccaga gttttgcgaa
      421 tgcgcaagac ctttccgtct actttgaaaa cacgttttcc ttcttcgttc tcgatccaga
      481 aatcatctcc gatggaaatg aggttttggc gaattttata acgggtggac atggcgaaat
      541 tccttttgaa attaaatttt ttcaatcagt ataatccatg tacgatagtg acttaactaa
      601 gttgcaatta attcgaaatg agttattgct tccaaacaat cagtaaaact ttgttgacga
      661 ccaaagcaat ggtatatacc cagccgaggg ttggtttgcc tgaagcaaac agggcaatag
      721 caccagaaga aaacagaatc agttccaagg tcaggaaaga agcgccggtt aaagggcgag
      781 ttgctctagg ggcaaggaaa gtcccccaca cgatggcgat cagaagaggg aagccaattc
      841 cgaaaagaat ctttggaaac caagtttggt gggttttgaa cccccagaag cctaccgcgg
      901 caagcataca caattccaat aaaaatctta atgctaaatt caattgtttt aaagcatcca
      961 tgttttactc caaattattt ctgtagccca gccaccagag cgagtaccag cctgcgaaaa
     1021 ctttcatcgc aatcttgcgg caagccaaaa ccgccagcca cttctaaagt ggcgaaacca
     1081 tgcaccatgc tgcgaaaggc acgtaaacca tgaatggcat tttcgccttt caatcccaat
     1141 gaagccataa cgaccaaacc gacattcacg gtgcgttctt cttctagctg taattcttta
     1201 tttggcgtct cttgcgcacc tgatgaacgc agggtcgaca tatacaaacc cggatattct
     1261 ttcacataag cacgaaaagc ttgtgatgct tccatgaata attcagtacc gctcttcccc
     1321 atcgccgctg aactgagccg atctgcaaga agttttgcat tgagtatggc aagttcttgt
     1381 tgcaagccag gcaacccttc tacatgattg tacaaagatg gcgtttgaat tcctaattct
     1441 tcggcaaggc ggctaagtgt gagcgcttgc acaccttctg cattgagaat ttctacagcg
     1501 gcttgcacga cagcattttt atcgagtcga gtacgagttt tgcgtggcat ggttatcctt
     1561 tcagtgaatt cataaagtcg agcatcagcg ggattgtcac ttcaggcatt tcggcatgtg
     1621 gataatgccc ggcattttcg atgattttat aatcgccgcg cactgcttct gcaactcgtt
     1681 tcgcttcggc aatcgggttt ttgaaatcag ggtctttgct gcccatcaag atcaacgcag
     1741 gttgttttac gttgggcata cgttcccctg aagaacgttt agaagctcgc atcattgcca
     1801 tcactgcctc taaccgccct agctgattga tgtttttata caatgtctca acataaaggt
     1861 cgaaatcagc gggtttgcgc gttggatata aggacgagta gtatttcttc cacatcgctg
     1921 caccccatgg gcgagcaaac atgattgaaa gcaagaaaat gaagaaagga ttgctatcgc
     1981 catccacgaa cggatcgatg agaagcatgc cgcgaattaa ctctggcgcc tctactgctg
     2041 accagatagc ggctcccccg cccatggaag ttcccactac gatagctgat tctgctttca
     2101 actcgcgtat gagtgcaaga atgtcttcac cgatggctgc cactgagaaa tcatcccaag
     2161 tggtactcgt ctcgccatgc ccgcggacgt ccatggtagc gacgcgatag ccagccttga
     2221 ccagttctgg tacgacaaaa cgatattcgc cgcgcacatc tcctaaagac ggcacacata
     2281 ccactaatgg accttctccc tgcacctcat aggcaatttg cccggtttta taattgaaaa
     2341 atcttgtttc catcgttctc tccatactac taattatatt agtattttaa ctaaatatat
     2401 tagtttttgt caagagggaa cttttctgta cgttatggcg tttattcact tcaactattt
     2461 atgaggagga agcatgtctt ttaaagtttc tgtgtatatt gccaccagtc tcgacggttt
     2521 catcgcccgc aagaacgggg acctcgactg gctgcccatc cccaccgaag gcggcgaaga
     2581 ttacggctac gctcaattca tgtccacggt ggatgtgatt gtgatggg
//
LOCUS       DAOQJE010000527         2626 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_27854_length_2626_cov_4.934656, whole genome shotgun sequence.
ACCESSION   DAOQJE010000527 DAOQJE010000000
VERSION     DAOQJE010000527.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2626)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2626)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2626
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27854_length_2626_cov_4.934656"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..991
                     /locus_tag="PLT08_12880"
     CDS             <1..991
                     /locus_tag="PLT08_12880"
                     /inference="COORDINATES: protein motif:HMM:TIGR03696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
                     /protein_id="HNE05412.1"
                     /translation="YGTVRDTSHLTPETLTTYGFAGEMADVTGLTYLRARYYSPLEGR
                     FMSRDTWEGDSYHPITLNKWSYAHGNPVKFTDPSGHDPWWCEYSSNPKQCEEDYWNSW
                     NIRCPTPEPISILPVSPYTFSDIDPDKWYEVNKGPVIEAFQWVSEFLGSKANTGLNLS
                     IGITFTFEETPIISGYRDTTGFADPWNHRIHFRGGEGDPPWDELRTIAIHEMGHFVDH
                     FAGNALGQGWYTSTNWVSGWEEIDGDWYWTGDEGAVPSDYVINKYAYSSQPNHWFCPH
                     GSNCFSEDFAETFTWMVYNSRGYNLADLDYRNNYNVPSPDRTDAVNKAIALLP"
     gene            1033..1752
                     /locus_tag="PLT08_12885"
     CDS             1033..1752
                     /locus_tag="PLT08_12885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE05413.1"
                     /translation="MSNKLSMIVMFILAILMFFACAPRATQVSSPAQNQDLSLENSPE
                     MLQETQLKWLNESPCQSPCWEGITPGITNKTDALSLLMQVENVNDVKVSPYESVGEIN
                     WYWGDNPLNSGRLFYSINNDVVYSVWPGIGNGLTLADIAQQFGPPTHAWVREVKPVEA
                     QQNQEEKNYSLEIVWLNKGFSVSGANPNHHGWIDAGFLIGSVKFFTPTHEGYILAVGN
                     VGKNLVPWHGYDTWENYIASE"
     gene            1903..2127
                     /locus_tag="PLT08_12890"
     CDS             1903..2127
                     /locus_tag="PLT08_12890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE05414.1"
                     /translation="MLTVDFKLSLPDELREEAEAAGLLNPTAIERLLREEIRRRRVDN
                     FFAAADRLAALDLPLMSDEEIAREIHLARG"
     gene            2136..2546
                     /locus_tag="PLT08_12895"
     CDS             2136..2546
                     /locus_tag="PLT08_12895"
                     /inference="COORDINATES: protein motif:HMM:TIGR00305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - endoribonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative toxin-antitoxin system toxin component,
                     PIN family"
                     /protein_id="HNE05415.1"
                     /translation="MNIVLDTNTVVSGLFWKGAPRQILDLARSGTFTLFTSPDLLSEL
                     ADVLGREKFSERLALAGTSVEELVFGYASLAKTVRPKKIAVVIKADPDDDKVLACAKA
                     AKAEIIASGDSHLLNLKEYEGIRIMTVNQLLEQY"
BASE COUNT          721 a          526 c          661 g          718 t
ORIGIN      
        1 gtatgggaca gtacgtgaca cctcgcactt gacccctgaa accctcacca cttatggatt
       61 cgcgggtgag atggcggatg tcacagggtt gacgtatctg cgggcgcgct attacagtcc
      121 gctggagggg aggtttatgt caagggatac gtgggagggg gattcttatc acccaatcac
      181 cctcaataag tggagctatg ctcatggcaa ccctgtgaaa ttcactgacc cgtctggtca
      241 cgacccatgg tggtgtgaat attcaagcaa ccctaaacaa tgcgaggaag attattggaa
      301 ctcttggaat atacgttgtc ctactccaga gccgatatca atattgccgg tatctccata
      361 tactttttct gatatagacc ctgataaatg gtacgaagtt aacaaaggtc ccgtcattga
      421 agcatttcaa tgggtttcag aattcttagg gtcaaaagca aatacaggct taaaccttag
      481 cataggaata actttcacct ttgaagagac gccaataata agcggataca gggatactac
      541 tggctttgca gacccatgga atcaccgtat tcattttaga ggtggagaag gagatccgcc
      601 ttgggacgaa ttaaggacaa tcgcaataca tgagatgggg catttcgttg atcattttgc
      661 tggaaatgca cttggccaag gatggtacac aagtacgaat tgggtctcag gttgggaaga
      721 gatagatggt gattggtact ggacaggaga tgaaggtgcg gtgcccagtg attatgtgat
      781 caataaatat gcttattctt cgcaacctaa tcactggttt tgcccacatg ggtccaattg
      841 tttctctgaa gattttgcag aaacgtttac atggatggtt tacaacagta gaggctacaa
      901 cttggccgac ttggattatc gaaataacta taatgtgcca agtcctgata gaactgatgc
      961 tgttaataaa gccattgcgt tattaccata attaggcttg agtttgtgtc tggcttgtaa
     1021 gaaaggaaat ttatgagcaa taaactgagc atgattgtta tgtttatttt agcgatcttg
     1081 atgttttttg cgtgtgcgcc gagagctacg caggtttcaa gtcctgctca aaatcaggat
     1141 ttgtcccttg aaaattcacc agaaatgctt caagaaacac aactgaaatg gctaaatgaa
     1201 agcccttgtc agagtccttg ttgggagggt attacccccg gcataaccaa caagactgat
     1261 gcgctttctt tgttgatgca ggttgaaaat gttaatgatg tgaaagtctc tccctatgaa
     1321 agtgtgggag aaataaattg gtattgggga gataatccat tgaatagcgg caggttattc
     1381 tattctataa acaatgatgt agtttattca gtatggcccg gtattggcaa tggattaacg
     1441 ttggctgata tcgcccaaca attcggcccg cctacacatg catgggtgag agaggttaag
     1501 cctgtggaag ctcaacaaaa tcaggaagaa aaaaactact cacttgaaat agtgtggtta
     1561 aacaaaggct ttagcgtttc aggtgctaat ccgaaccacc atggttggat tgatgcaggt
     1621 tttttaatcg gttctgttaa gttttttacc ccaacacatg aaggatacat attagctgtg
     1681 ggcaatgttg ggaagaacct tgtgccttgg cacgggtatg atacatggga aaattatata
     1741 gcttcggagt agaagtttgt ataggtatga taattgatgt tgttgatggg cttactctct
     1801 gttcaaggca gcgtctccgc cgccgacgat gttaccttga gcagttagtt agtcattttt
     1861 tccaaatgat ataatatttt tagagacaga aagaaggtca aaatgctgac agtggatttc
     1921 aaattatcgt tacctgatga gttgcgcgaa gaagcggagg cggctgggtt attaaaccca
     1981 acagccattg aacgcctttt gcgcgaagag attcgccgcc gccgcgtgga taactttttt
     2041 gccgccgccg accgcctcgc ggcattggat ttgcccctga tgtccgatga agagattgcg
     2101 cgggaaatcc atctggcgcg tggataatcc tccaaatgaa tatagttctt gacacgaata
     2161 ctgtagtctc aggtttgttt tggaaaggcg cgcctcgtca aattcttgac ttggcgcgtt
     2221 ctggcacatt tacactcttc acaagccccg acctgctttc agaactggcg gatgtgcttg
     2281 ggcgggaaaa gttttctgaa cggcttgcac tggcgggaac cagcgttgag gaattggtct
     2341 ttgggtatgc ctccctggcg aagacagtcc gcccgaagaa aatcgcggtt gttataaaag
     2401 ccgaccctga cgatgacaaa gtgctggcat gtgccaaagc ggcaaaggcg gagatcattg
     2461 cctcagggga tagtcacttg ctcaatttga aagaatatga gggaatccga attatgacag
     2521 ttaaccaact ccttgagcag tattgataat tcctctagtt gaccgactcc acaagcacag
     2581 tgactcacgc caacggagcg gctcaatccg cgtatggatt cacggg
//
LOCUS       DAOQJE010000528         2625 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_27873_length_2625_cov_4.179767, whole genome shotgun sequence.
ACCESSION   DAOQJE010000528 DAOQJE010000000
VERSION     DAOQJE010000528.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2625)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2625)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2625
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27873_length_2625_cov_4.179767"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..408)
                     /gene="amrB"
                     /locus_tag="PLT08_12900"
     CDS             complement(<1..408)
                     /gene="amrB"
                     /locus_tag="PLT08_12900"
                     /inference="COORDINATES: protein motif:HMM:TIGR04336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system protein B"
                     /protein_id="HNE05416.1"
                     /translation="MNTLDLRPSPIAGTWYPKQPEVLANSIDEYLKAVKLPTLQGQVV
                     GVIAPHAGHRYSGAVAAHAFATLRGLTPDLVVILSPFHNFDPHPLLITKHQAYATPLG
                     NIEVYQHTINELQSYLHIPITPIANDKEHSLEIE"
     gene            complement(481..1146)
                     /locus_tag="PLT08_12905"
     CDS             complement(481..1146)
                     /locus_tag="PLT08_12905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE05417.1"
                     /translation="MKSHRFFFGFMALLVAVSLACGGSGSPTANPTLPPVPTNPPAQQ
                     QDQGQQQQDQGQVPSDGTILTYTDQNNLWAFDLPGDWQHEYKDLGDTLYTDATAYLDT
                     FTSPDGSAYIDSLVMFANEGVTVNNSTSQAVALDLLNTYYSSTGKNNGDIRIKSDQIM
                     QDGSERFEWVSKGGNYFGTTFFEVRGSDKRTWLMWTLVADNNADQAVLDILDNAIASY
                     RIP"
     gene            complement(1409..1930)
                     /locus_tag="PLT08_12910"
     CDS             complement(1409..1930)
                     /locus_tag="PLT08_12910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE05418.1"
                     /translation="MNTSLPEQKPGLVNAIAWMTLASGIINLFWGFVASATALGTIVG
                     VICLPITILPTILGIFEIIYAAKLLSAQPQPVQPSNSIAVFEILTFLMGNVFSMVVGI
                     LSLIFYNDLTVKAYFARINAGNAVAPEPVIVSTPAQIEEPQPEILPEPIVEIIPEEPI
                     QPAKPKKPRKTSK"
     gene            complement(1994..>2625)
                     /locus_tag="PLT08_12915"
     CDS             complement(1994..>2625)
                     /locus_tag="PLT08_12915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE05419.1"
                     /translation="RMLAAGESFDEARPLAQLATDLQQGYVSEPFTPTPFPTFNTPNA
                     QFTSTPTESGDQPEVSEEATFTPAAPTLLFEQTPITPIVQSTLTQRPTFTPTAGPGAP
                     FALVNQETVCDLGMSRVMQFMLLNSKREQVPGAEIIVTWSQGEDRFFTGFKPELGNGY
                     ADFLMESNTTYNIRVVTGGAFVADVRAPDCTDPNGATYSGGLVLTFQQP"
BASE COUNT          669 a          546 c          711 g          699 t
ORIGIN      
        1 ctcaatctca agcgagtgtt ctttatcatt ggcgataggt gtaatgggga tatgtaagta
       61 agattgaagt tcgttaattg tatgttgata tacttcaata ttgccgagcg gagtggcata
      121 ggcttggtgt ttggttatca ggagcggatg tgggtcaaaa ttatgaaaag gggagagtat
      181 tactacaagg tcgggggtaa gtccgcgcag ggtggcgaag gcgtgtgcgg caacggctcc
      241 tgaatagcgg tgacccgcat gtggtgcaat cacacccacg acctgccctt gaagtgttgg
      301 aagtttcacc gcttttagat attcatcgat actgtttgcc aacacttcag gctgtttcgg
      361 ataccaagtt ccggcaattg gggaagggcg tagatcgaga gtgttcataa ttttattgta
      421 gcacttgcag aaataaaaac tccccgcgaa ttttcgcggg gagttaaatt actggctaaa
      481 ttacggaatg cggtaggaag cgatggcgtt gtcgaggata tccaacacag cctgatctgc
      541 attgttatct gctacgagag tccacatcaa ccaagtgcgt ttgtcactgc cacggacttc
      601 gaagaaggtc gtaccaaagt agttgccgcc tttggatacc cactcgaagc gctcggatcc
      661 atcttgcata atctggtcac tcttgatgcg aatgtcgccg ttattcttgc cggtgctgct
      721 gtagtaggtg ttgagcaggt ccaacgccac agcctggctg gtggagttgt tcacggtcac
      781 accttcgttt gcgaacatga cgaggctgtc gatgtatgcg gaaccatcgg gggaggtgaa
      841 ggtatccaag taggcggttg catcggtgta aagggtgtcg ccaaggtctt tgtattcatg
      901 ctgccagtca ccggggaggt caaatgccca cagattgttc tggtcagtat aggtgaggat
      961 ggtgccgtcg ctgggcactt gtccctgatc ctgttgttgc tgaccttgat cttgctgctg
     1021 ggcgggggga ttggtgggga cggggggcaa ggtggggtta gcggtagggg aaccggaacc
     1081 accacatgcc aggctgacgg ctactaacaa cgccataaag ccaaaaaaga aacgatgtga
     1141 tttcataatt ttgtattctc ctctatcttt attgatgatg gacgcaagga taccctatga
     1201 ccttattttt ttgaggggta ccttagtccc attttacacg ccctgccatt cgttgcatac
     1261 atctggaatt cggattagaa atgctacgaa aaagcaggga atccgatgct agaagaaatc
     1321 acagcatgga acttgatttt tttgactgcc tataaacgac aaaggctgtg aaaaagttcc
     1381 atgctgtgga gctaatttca agatgaattt atttagacgt ttttcgtggc tttttaggct
     1441 ttgcaggttg aattggctct tcaggaataa tttctacaat tggttcaggg agaatttcgg
     1501 gctgcggttc ttcaatttga gcaggtgttg aaacaatgac cggctctggc gccacagcat
     1561 ttcctgcgtt gatacgagca aagtaagcct taaccgtgag atcgttgtag aagatcagag
     1621 agagaatacc caccaccatt gagaaaacat ttcccattaa gaacgttaaa atctcaaaaa
     1681 cggcaatgga gtttgaaggt tgaacaggct ggggttgagc acttaataat ttggcagcgt
     1741 aaatgatctc gaaaattcct aagatcgtag gaaggatagt gatcggtaaa caaatgacac
     1801 caacgattgt gccaagtgca gttgcagatg caacaaaccc ccaaaaaaga ttgatgatgc
     1861 cactggcaag cgtcatccat gcaatcgcat taacaagccc gggcttttgt tcaggaagag
     1921 aggtattcat gtattgctcc ttaagatttg cttgttgaat ataacgattg aacacacgat
     1981 tggttgcaaa tcattaaggt tgctggaatg taagcacaag cccgcccgaa taggttgcac
     2041 cattggggtc tgtgcaatct ggtgcgcgca catctgccac aaaagctcca ccggttacaa
     2101 cccgaatgtt gtaagtagta ttagattcca ttaagaaatc tgcatatcca tttccaagct
     2161 cgggtttgaa gccagtaaag aaacggtctt cgccctggct ccacgtcaca ataatttcgg
     2221 cgccaggcac ttgctctcgt tttgaattta agagcatgaa ttgcatcact ctagacatcc
     2281 ccaggtcgca aactgtttct tgattgacca atgcaaacgg agcgccgggt ccggctgttg
     2341 gtgtgaaggt agggcgctga gtcaatgtgg attgaacaat gggcgtgata ggagtctgtt
     2401 caaagagaag ggtaggcgct gctggtgtaa acgtcgcctc ttcactgacc tcgggttggt
     2461 ctccgctttc agtgggcgtg gatgtgaact gggcgttcgg tgtgttaaaa gtggggaaag
     2521 gtgtaggcgt aaacggctcg ctcacatacc cttgttgaag gtctgttgct aattgcgcaa
     2581 gcggacgtgc ctcatcaaat gattcgcctg ctgccagcat acgct
//
LOCUS       DAOQJE010000529         2621 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_27949_length_2621_cov_7.854638, whole genome shotgun sequence.
ACCESSION   DAOQJE010000529 DAOQJE010000000
VERSION     DAOQJE010000529.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2621)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2621)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2621
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27949_length_2621_cov_7.854638"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..195)
                     /locus_tag="PLT08_12920"
     CDS             complement(<1..195)
                     /locus_tag="PLT08_12920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE05420.1"
                     /translation="MRRLFLPLLLTVLACGGNGIPGPTPTPLSPTATVSAPQTESQPQ
                     ASPATLPPTAVVIESAQQFPD"
     gene            complement(196..567)
                     /locus_tag="PLT08_12925"
     CDS             complement(196..567)
                     /locus_tag="PLT08_12925"
                     /inference="COORDINATES: protein motif:HMM:NF018508.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1232 domain-containing protein"
                     /protein_id="HNE05421.1"
                     /translation="MTEKKSSDIIVNQQGGVVRNLVNQLKLIFRLMGDKRVSVLAKLV
                     PLGAFAYLIMPADLAPNVVLPVIGMVDDAAILWLGTYIFTELCPPEVVEEHMKALAGN
                     MNPKDVNNEDVVDGETTEVKE"
     gene            787..1179
                     /locus_tag="PLT08_12930"
     CDS             787..1179
                     /locus_tag="PLT08_12930"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HNE05422.1"
                     /translation="MDMIIDFPGGQKVDAHFRGHTIKTDQPNDNTAPMPFELFLASIG
                     TCAGIYVLGFCQQRNLPTAGIRIVQRNYSNPANGLVDKIDLEIQVPPNFPEQYHAALV
                     RTAELCKVKKTLEHPPVFDVMTKVVEAA"
     gene            1264..1731
                     /locus_tag="PLT08_12935"
     CDS             1264..1731
                     /locus_tag="PLT08_12935"
                     /inference="COORDINATES: protein motif:HMM:NF017774.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HNE05423.1"
                     /translation="MPYIIDGHNLIPKLGLRLDEPDDEMELVRLLQDFARIRRQQIDV
                     YFDGAPAGQAGMRKFGSIKAHFVRQGQTADSAIRRRLEEMGKAAKNWIIVSSDHEVQG
                     AARVHMAQAIRSEEFVKQIRIAFSTSTQTGASDSKLSAQEVEEWINLFSKKKE"
     gene            1969..>2621
                     /locus_tag="PLT08_12940"
     CDS             1969..>2621
                     /locus_tag="PLT08_12940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE05424.1"
                     /translation="MVSNDVVVQRSYVYFVRWCMAVVITMGVFACSYIPSLPSPTSFT
                     ECIPSSYTANIYRPTPTPTFPVAVASTSIPNIVSTAQPMPVDIHLHFQKLIQEVSRWT
                     AITPFPLGDSSEVWVAITFLSPELLRTVSTTDLLSENPAGINLDIQVNNMLAEIAKRE
                     KLLFFLTAFKANTGGEATNSHTFQIDVENLKLINAKNLGIKPAYYDHNLGQKMEFPAP
                     "
BASE COUNT          738 a          612 c          588 g          683 t
ORIGIN      
        1 gtcggggaat tgctgggcgc tttcaatcac caccgcggtt gggggaagag tggcggggga
       61 cgcttgaggt tgagattcgg tttgtggtgc tgaaacagtt gccgttggag aaagaggcgt
      121 gggggtgggt ccgggaatgc cattccctcc gcaggcaagg acggttaaca ataaagggag
      181 gaaaagacgt cgcatttatt cttttacttc agtggtttca ccatccacaa catcttcatt
      241 gtttacgtct ttggggttca tattcccggc aagcgccttc atgtgctctt ccacaacttc
      301 tggcgggcac aattcggtga agatataggt gccaagccag agaatggcgg catcatcgac
      361 catgccaatt acaggcaaga cgacgttcgg cgcaagatca gcaggcataa tcaaataagc
      421 aaaagcgccc aagggcacta actttgccaa gacactgacg cgcttatcgc ccatgagacg
      481 aaagataagc ttcagttggt tcacaaggtt gcgcaccacc ccgccttgtt ggttgacgat
      541 gatatcagag gattttttct cagtcatttt gttctccttt gagttgtatg ttcattatat
      601 tcggaattgc aagacatcca ctttcgatta ttgcaatcta tatgaacata gattgatata
      661 cgtgacaaaa gaagatgggg tgcaggtaaa aatggcagaa gcaggaaaaa aactcaaaaa
      721 ttgggtgtaa cttattctta gcaaatacat ccaatatgta caacaataaa tttggaggat
      781 ttccccatgg atatgattat agatttcccc ggcgggcaaa aagtagatgc ccacttccgc
      841 ggtcacacca ttaagacaga ccaacccaac gacaatactg ctccgatgcc gtttgaattg
      901 ttccttgctt ctattggtac ctgtgcaggc atttatgtgc taggcttttg ccaacagcgc
      961 aacctgccca ctgcaggcat tcgcatcgtt cagcgcaatt attccaaccc ggcaaacggg
     1021 ttggtcgata agatcgatct tgaaattcag gtaccaccca acttccccga acaataccat
     1081 gccgcactcg tgcgcactgc agagttatgc aaagtgaaga agactcttga acatccacct
     1141 gtgtttgatg taatgaccaa agtggtcgaa gctgcctaag aaaccgaaga taaaaagacc
     1201 gcagacattc acagtctgcg gtctttttat tccaaaacaa ctcaagcaca gtaaaatgaa
     1261 cccatgccct acattattga tggacataac ctgattccca aacttggatt acgtttggat
     1321 gaacccgatg acgaaatgga actcgtgcgc ctcttacaag actttgcacg gattcggcga
     1381 cagcagattg atgtctactt tgacggcgct cccgccgggc aagctggcat gcgaaaattt
     1441 ggcagcatca aagcgcactt tgtaaggcaa ggtcaaactg cagattcagc catccgcagg
     1501 cgattagagg agatggggaa agcagcaaaa aattggatca ttgtttcttc agaccatgag
     1561 gtgcaaggtg cggcacgagt gcatatggca caagcgattc gctcggaaga atttgtcaaa
     1621 caaattcgca ttgccttctc tacaagtacc caaacgggtg catctgactc aaagctttcg
     1681 gcgcaagaag tggaagagtg gataaatcta tttagtaaaa agaaagaata gtacctttgt
     1741 cttatacgtt tttaatgaaa tgtggacaaa aaaggtgtat attgtctgta cgacacctgc
     1801 cctccctttt actggtattc tggtgtcaac cgccgctctg gtcaacatgc cccccccatg
     1861 ctgacaggcg gcggtacctt tttaacaatg ccaaaataca cttgaagaag gtctgagagg
     1921 tcaatataat attttctatc cgatcaaatc aatgtggagg cgggcttgat ggtttcaaac
     1981 gatgtagtgg tacagcgttc ttatgtgtat tttgtacgat ggtgtatggc ggttgtgatc
     2041 acaatggggg ttttcgcatg cagctatatt ccttctttac catcccccac ctcttttaca
     2101 gaatgtatcc cttcatctta tacagctaat atctatcgtc caacaccaac gcccaccttt
     2161 cctgttgcag tagcatccac atcgattccc aatattgttt cgactgctca gcccatgcca
     2221 gttgatatcc atctgcattt tcaaaagtta attcaagaag tcagccgctg gacggcgata
     2281 actcctttcc cacttggcga ttcaagtgaa gtttgggttg cgatcacatt tctaagccct
     2341 gagcttttac gaaccgtctc tactaccgac cttttgagtg aaaacccagc cggtatcaat
     2401 ttagatattc aagttaacaa tatgcttgca gagattgcca agcgcgagaa actattattt
     2461 ttcttaacag ccttcaaggc gaacacaggt ggcgaagcca caaactcgca cacgtttcaa
     2521 attgatgtag aaaatctaaa gcttatcaat gccaaaaacc tcggtattaa acctgcatac
     2581 tatgaccata accttgggca aaaaatggaa ttccccgcgc c
//
LOCUS       DAOQJE010000530         2621 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_27954_length_2621_cov_5.063913, whole genome shotgun sequence.
ACCESSION   DAOQJE010000530 DAOQJE010000000
VERSION     DAOQJE010000530.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2621)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2621)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2621
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27954_length_2621_cov_5.063913"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..688
                     /locus_tag="PLT08_12945"
     CDS             <1..688
                     /locus_tag="PLT08_12945"
                     /inference="COORDINATES: protein motif:HMM:NF013481.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016625 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, iron-sulfur
                     protein as acceptor [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aldehyde ferredoxin oxidoreductase C-terminal
                     domain-containing protein"
                     /protein_id="HNE05425.1"
                     /translation="WQVNDLGLDTIEVGGALGVAAEAGIMRWGSEEDGLKLIDEIRKG
                     TELGRVLGDGAVAVGKKYGVERVPAVKGQSMSAYEPRSIKGTGVTYATTPQGADHTCG
                     LTIRAQVNHLDPTQQKDASLNAQLNMAGYDTIGACIFAGFGYAATHDGVVKRLLKARY
                     GWEDVPDNVLQALGKETIKLEREFNKRAGFTKEDDRLPKWMMEEALPENGSVFDVSED
                     VLDHIFDGIE"
     gene            complement(756..2333)
                     /locus_tag="PLT08_12950"
     CDS             complement(756..2333)
                     /locus_tag="PLT08_12950"
                     /inference="COORDINATES: protein motif:HMM:NF012398.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HNE05426.1"
                     /translation="MEREKLAFFNPATNTKFGEVVVNTPEEVQQAVREMRQAFPEWSR
                     KSIQERADILHKLQHVLIEQRDEISNVLNMDCGKSRQDGLLELFVAIDMLVQYRHNAK
                     KWLRRQRVSSGLYIFKRCYIEHRPHGVTAIISPWNYPFTLSLPPMVSALLAGNTVVLK
                     ASEVTAATGVLIEKLIKSVPELAPFVRVVHGDGRVGAALVASKPDYIFMTGSTATGKK
                     IMQTAAETLTPVALELGGKDAMIVLEDAELEAAARWGAWGAFFNTGQTCMSVERVYVV
                     ESKYDEFVNLAVQQAQELKSGYSTELNSPFFMGPVTDQRQVKIIDGHLQDALDKGAKI
                     LTGGKRDGMFYDPIVLVNVDHSMKIMRDETFGPVMPIVKVKNEEEAIHLANDNAYGLG
                     ASIWSNDISHAKRVADRVEAGSIIINDSIAQFGVPMLPFGGTKDSGFGRTHGKEGLLQ
                     FTRPYSFAIGGAPVKMDVATVLREMGHYELATMIMGVLFGTTLKQKVETFLQFFRRGK
                     KQAPPAGETSSNTAAAD"
     gene            complement(2440..>2621)
                     /locus_tag="PLT08_12955"
     CDS             complement(2440..>2621)
                     /locus_tag="PLT08_12955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE05427.1"
                     /translation="FALHQVINAVSMPLLINGFIEVKGPLAPVFTPTNEGLFVSLLFG
                     VTGWLLMKKRLQTQA"
BASE COUNT          676 a          586 c          650 g          709 t
ORIGIN      
        1 ttggcaagtt aatgaccttg gtttggatac catcgaagta ggtggagcat taggagtcgc
       61 cgccgaagca ggaattatgc gctggggcag tgaagaagac ggcttgaaat tgattgatga
      121 aattcgaaaa ggcacagaac ttggacgtgt ccttggcgac ggggcggtag cagttggaaa
      181 gaaatatgga gttgaacgtg ttcctgcggt caaaggtcag tctatgtccg catatgaacc
      241 gcgttccatt aaagggacgg gtgtgactta tgcgaccact ccgcaaggtg cagaccatac
      301 ttgtgggtta acgatccgtg ctcaggttaa tcaccttgac cccactcagc aaaaggatgc
      361 gtctctcaac gcgcagttga acatggcggg ctacgatacc atcggtgcat gtatttttgc
      421 aggcttcggt tacgcggcaa ctcacgatgg ggtagtgaaa cgtctattga aggcgcgtta
      481 tggttgggaa gatgtgccgg ataacgtgtt acaagctctt ggcaaggaga ctattaaact
      541 agagcgtgag ttcaacaaac gcgcagggtt taccaaagaa gatgaccgtt tacccaaatg
      601 gatgatggaa gaagccttgc cagaaaatgg ttctgtcttt gatgtgagcg aagatgtgct
      661 tgatcatatt tttgatggaa ttgaatagaa gaacataagt agtctgcaag attgagaata
      721 aaaactgccc tgtagaattc ctacagggca gttttttaat cagctgcagc tgtattggaa
      781 ctggtctcgc cggctggcgg ggcttgtttt ttgcccctac ggaagaattg taaaaaggtc
      841 tctacttttt gtttgagggt ggtgccaaat agaactccca taatcatggt tgctaactca
      901 tagtgtccca tttcgcgcaa aacggttgca acatccattt tgacgggcgc accgccaata
      961 gcaaagctat atggacgagt aaattgtaat aagccttctt tgccgtgtgt gcgaccaaaa
     1021 cctgagtcct ttgtgccgcc aaaaggcagc atgggaacac caaattgagc gatactatca
     1081 ttgatgatga tcgatcccgc ctcaactcgg tccgcaactc gtttggcatg gctgatatcg
     1141 ttgctccaaa tggaagcgcc taaaccataa gcattgtcat ttgcgaggtg tatggcttct
     1201 tcttcgttct tcactttaac tatgggcata accggaccaa atgtttcatc gcgcataatc
     1261 ttcatgctgt ggtctacatt gacaagcaca atcgggtcat agaacatccc gtcacgtttg
     1321 ccgcctgtaa gaattttggc gcctttatcc aacgcatctt gcaaatgtcc atcaatgatt
     1381 ttaacttggc gctggtcagt gacgggtccc ataaagaatg ggctattgag ttcggtggaa
     1441 taaccgctct ttagctcttg tgcctgctgc actgccagat ttacaaactc atcatatttt
     1501 gactcgacca catagacacg ctctacagac atgcatgtct gaccggtatt gaagaaggct
     1561 ccccaggctc cccaacgagc agcggcttct agctccgcat cttcaagcac gatcatggca
     1621 tctttcccgc caagttctag ggctacgggg gtaagtgtct ctgctgcggt ttgcatgatc
     1681 ttcttgccgg tggctgtgga gccagtcata aaaatgtagt ctggcttgct ggcaaccaag
     1741 gctgcaccca ctctgccgtc accatgaaca acacgtacaa atggcgctaa ctctggtacg
     1801 ctcttaatga gcttttcgat caatacgcct gtagcagcag tcacttcaga ggcttttaga
     1861 actacagtat tcccagcaag taaggctgag accatgggtg gtagagataa tgtaaatggg
     1921 tagttccatg gtgagataat ggcggtcaca ccgtgggggc gatgttcaat gtaacagcgt
     1981 ttgaaaatgt aaagcccaga ggaaacccgt tgacggcgga gccatttttt agcattgtgg
     2041 cgatattgga caagcatgtc tatagcaacg aataactcaa gcaacccgtc ctgacggctt
     2101 ttgccacagt ccatgttgag cacattgctg atttcgtcac gttgctcgat tagcacatgt
     2161 tgcaacttgt gaagaatgtc cgcccgttct tggatgcttt tacggctcca ttctgggaat
     2221 gcctgccgca tttcgcgaac ggcttgttgc acttcttcag gagtgtttac gacaacctca
     2281 ccaaactttg tgttggtggc aggattgaaa aatgctagct tttcgcgttc catgaaaggt
     2341 tccttggcgt ttgaaattgt ttttcttttc caattataac cagttgttta tagaaattgg
     2401 ctccaaaaag tgagaaaaga gtctgttttt tcctctttat tatgcctgag tttgcaatct
     2461 ttttttcatc agtagccagc ctgtgacgcc gaataagagc gaaacaaaga gcccttcatt
     2521 tgtaggcgta aagaccggcg caagcggacc tttcacctcg ataaacccat taataagcaa
     2581 aggcatactg accgcattaa tgacttggtg tagcgcaaaa a
//
LOCUS       DAOQJE010000531         2619 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_27999_length_2619_cov_5.795632, whole genome shotgun sequence.
ACCESSION   DAOQJE010000531 DAOQJE010000000
VERSION     DAOQJE010000531.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2619)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2619)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2619
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27999_length_2619_cov_5.795632"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(160..1122)
                     /locus_tag="PLT08_12960"
     CDS             complement(160..1122)
                     /locus_tag="PLT08_12960"
                     /inference="COORDINATES: protein motif:HMM:NF024676.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YafY family protein"
                     /protein_id="HNE05428.1"
                     /translation="MTNTATRLITLIFLLQNQPNQKALELAEKLGVSLRTVHRYFEML
                     DEMGVPVYSERGPYGGFSLVRGYKMPPLVFTLEEAVAVVLGTGLVQELWGDLYRESAN
                     GALAKLENLLPEEQTREVAWARSSLAVTGLHRANLKMQTPALEKLRRAIRELRSVNML
                     YQTSQVPHPAERGLDPYALVHRWGWWYVIGLCHVHNEVRTFRVDRISEVTLSENIFVK
                     STGFDLQEYLNIEMQSQPQITARLKFDVVSANIVSGNQSYWQNVEPQADGSVEVTFLS
                     PTLEWAASTVLMYGPAVEVLEPPALRSMVAEWLEVTIRKYTEKE"
     gene            complement(1203..2363)
                     /gene="dinB"
                     /locus_tag="PLT08_12965"
     CDS             complement(1203..2363)
                     /gene="dinB"
                     /locus_tag="PLT08_12965"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF002677.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HNE05429.1"
                     /translation="MARTILHLDLDAFYCSVEETQNPQLRGKPFAVGGRPDERGVVAS
                     CSYAARALGIRSAMPMSRAVRLCRDLIIVPGRHHLYGEYSAKVMEKLHNLTPLVEQIS
                     IDEAFLDISDIQADKTRLALDLQLSIRNELNLPSSIGIASNKLVAKIATEVGKKSSKK
                     KNEPPFGFTVVPVGDEAKFLDPLPADMLWGVGPKTNARLAELGIHTIGDIARWPEKEL
                     VSLFGENGRDLWQHAHGMDNRPVSTESETKSISQETTFNVDISDQKTLEKTLREQAHE
                     VARQLRKNDLAGKTVKIKIRWPDFTTLTRQTTLPTSTDNEDDIYRAAVKLMDSVRKPN
                     QAVRLLGVGVSGIGAPVRQLSLWDVGSEKSRKLQEVVDELQEKYGKDVIWKG"
BASE COUNT          625 a          623 c          636 g          735 t
ORIGIN      
        1 gagcaaatga tggcgatttg cccggttcgg agcctttttc gatggcaccg tcgaccatgg
       61 caaattgcag gttcggcact tgtaccaatt cgatcctttt tgccgagggt tgatatagat
      121 ttttatattg tttttttaga tctaagattt tcatagtctt cattcctttt cggtatattt
      181 tcggatggtg acttcaagcc attcagccac catcgacctc aacgctggcg gttccagcac
      241 ctccacggcg ggaccataca tcaacacggt gctggctgcc cattccaacg tgggagatag
      301 gaaagtaact tctacagagc catctgcttg cggttcaaca ttctgccaat aggattgatt
      361 ccctgaaacg atgttcgctg aaactacatc aaatttcaaa cgcgctgtga tctgcggctg
      421 agattgcatt tctatgttta aatattcttg caaatcaaaa cctgttgatt tgacaaagat
      481 attttctgac agcgtaactt cggaaatacg gtccacgcgg aatgtgcgga cttcattgtg
      541 aacatggcag agcccgatta cataccacca gccccaacga tggacaagtg catacgggtc
      601 aagcccgcgt tctgcagggt gcggaacctg actggtctga tagagcatat tcacactgcg
      661 aagctcgcga atggcacggc gtaatttttc tagcgcaggt gtttgcattt ttagattcgc
      721 acggtgcaat cctgtaacgg caagtgaact gcgagcccag gcaacttcac gcgtctgttc
      781 ttctggcaaa agattttcca gtttcgccaa agctccattt gcagactcgc gatataagtc
      841 tccccacaat tcttgcacca aacctgtgcc caatactaca gcaacggctt cttccaacgt
      901 aaagaccagc ggcggcattt tgtatccacg cacgagcgag aagccaccat acggtccgcg
      961 ttcagaatag accggaacac ccatttcatc cagcatctca aaatagcggt gaactgtccg
     1021 caatgagacg ccaagtttct cagccaattc caaagctttt tggtttggct ggttttgtaa
     1081 tagaaaaatg agcgtaatga ggcgggtggc agtgttggtc atgtgttaca tccttcaata
     1141 atataaaacg ggtatatgtc aaattatgac atatacccgt tagcaatttt tgggtttgag
     1201 cactagcctt tccatatgac gtctttgcca tacttctctt ggagttcatc cactacttct
     1261 tgtaacttgc gggacttttc gcttcctacg tcccataagc tgagttgacg aaccggagct
     1321 ccgattccgc tgacgccgac tcctaacaac cgcacggctt gatttggctt gcggacggag
     1381 tccatcaact tgacggcggc gcggtagata tcgtcttcgt tgtctgtaga agtgggtaag
     1441 gtggtttgtc ttgttagggt ggtgaagtcg ggccagcgga tttttatttt gactgttttt
     1501 ccggctaggt catttttgcg aagttgccga gccacttcgt gagcttgttc gcgcaatgtt
     1561 ttttctaagg tcttttggtc gctgatatct acgttgaacg tggtctcctg gctaatagac
     1621 ttggtctctg attcggtact gactggacga ttatccatgc cgtgtgcatg ttgccagagg
     1681 tcgcgtccat tttcgccgaa gaggctaacg agttcctttt cgggccagcg ggcaatatcc
     1741 ccaatggtat gaatgccaag ctcggctaac cttgcattgg tttttggtcc cacaccccaa
     1801 agcatatcag cggggagtgg gtctagaaat ttggcttcat ctccaacagg aacaacggtg
     1861 aagccgaatg gtggctcatt ctttttcttg cttgatttct taccaacctc agtggctatt
     1921 tttgccacga gtttgttgga cgcgatccca atgctcgagg gtagattcag ctcatttcgt
     1981 atggacagtt gcaggtcgag cgcgaggcgg gttttatccg cttgaatatc cgagatgtcg
     2041 aggaaggctt cgtcgatgga aatttgctcc acgagtggag tcaggttatg cagcttttcc
     2101 atcaccttgg cagagtattc tccatacaaa tggtgccgcc cgggtacgat gatcaaatca
     2161 cgacataggc gcaccgctcg cgacatgggc atggcactcc gaatcccaag cgcgcgagcc
     2221 gcatatgaac atgatgcgac cacaccacgt tcatcaggtc tgccaccaac ggcaaacggt
     2281 ttgccgcgca gttgcgggtt ttgggtctct tccacggagc aataaaatgc atcgaggtca
     2341 aggtgaagga tcgtgcgagc catgttggta ttatagtgca gtcgattttg ccctaagagc
     2401 acagaaaaat gggaaaaatt gactatgatt ttgttctgtt tcattattga acaattatta
     2461 aagaaaagtt atactatggt agcgtatagt caaaagggaa cttttcactt aatcactacg
     2521 tccatacccc gtatgactga tggaggaaga aatgagaact attgtgagat cgatttcaac
     2581 attactcctg ttggcggtgc tattcagcct cgccccagg
//
LOCUS       DAOQJE010000532         2619 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_28000_length_2619_cov_5.623245, whole genome shotgun sequence.
ACCESSION   DAOQJE010000532 DAOQJE010000000
VERSION     DAOQJE010000532.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2619)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2619)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2619
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28000_length_2619_cov_5.623245"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(15..782)
                     /locus_tag="PLT08_12970"
     CDS             complement(15..782)
                     /locus_tag="PLT08_12970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE05430.1"
                     /translation="MNVLALKLILAPLIIGSASLAGRRWGPAVSGWIVGMPLTSGPVV
                     FFVALSHGATFAESSVLGVLSGGISLVLYALTYAWVATRFPWYVAFVGSFIVFSLSTL
                     FLQNITVSLWFIVPVVVAAILIGLTYMPKGEVEPGESKLSQWDIPVRILIGTSFILLL
                     TGIAPFIGSRLTGLLTTIPLYVTILSIFAHRDYGPAAAAHVLRGLLYGMFAFTGFFFV
                     LSLLIEETSLGVSFGLAVLAALAIQGTSLLILRRSHQ"
     gene            complement(782..1399)
                     /locus_tag="PLT08_12975"
     CDS             complement(782..1399)
                     /locus_tag="PLT08_12975"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014566.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0070007 - glutamic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family glutamic-type intramembrane
                     protease"
                     /protein_id="HNE05431.1"
                     /translation="MKSKLFLAIPPYIAVWVGMFVLKNAWAALLGFHIAILIVIFLLK
                     PQYSFRTFFQSTSKKLFFFTILLCALSGPSLYLLRDFLGISPNLRVQLTALGLQGNVW
                     EWFILYFSIINPFMEEFFWRIVLGSDIKSFYLGDLVYAGYHTMIVWNKTSPPLSIVLV
                     LLALTFIGWLWRQLYRKDGSLLTPVVSHSIADLSILLAIFFLVNP"
     gene            complement(1407..2330)
                     /gene="mvk"
                     /locus_tag="PLT08_12980"
     CDS             complement(1407..2330)
                     /gene="mvk"
                     /locus_tag="PLT08_12980"
                     /EC_number="2.7.1.36"
                     /inference="COORDINATES: protein motif:HMM:TIGR00549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004496 - mevalonate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016126 - sterol biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019287 - isopentenyl diphosphate
                     biosynthetic process, mevalonate pathway [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mevalonate kinase"
                     /protein_id="HNE05432.1"
                     /translation="MKSSAPGKIILFGEHAVVYNRPALAVPVNQVQVDVEVLDSDKPG
                     VWINAPMVNLHGELSTLPSDHPIGSVILKVFQHLGISPQPDLKLTIYSTIPVAAGLGS
                     GAAVSVALIRALSSFLNHSLSNEQVNELVFEIEKLHHGTPSGIDNTVITYNMPVYYMK
                     GKPIETFKCGKPFTIVIGDTGVPAPTKESVGDVRRLWQRDTNHFENIFNEVAQISTMA
                     RHFIESGRPEHLGELMDHNHEFLQKMTVSSPELDRLVDAARKAGALGAKLSGGGRGGN
                     MIALVNQDSAELVAAALMSAGAKRTVITEVK"
     gene            complement(2327..>2619)
                     /locus_tag="PLT08_12985"
     CDS             complement(2327..>2619)
                     /locus_tag="PLT08_12985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="decaprenyl-phosphate phosphoribosyltransferase"
                     /protein_id="HNE05433.1"
                     /translation="VLDGYTLPLLDQYIMIVSGTTIVAYSLYTFSAPNVPENHSMMLT
                     IPFLVYAIFRYLYLIEVEHAGGTPEEVLLSDRPFQISMLLWAVTVIIIFYFS"
BASE COUNT          812 a          582 c          633 g          592 t
ORIGIN      
        1 accaagcccg ttttttattg atgcgacctt cgtaatatca gcagcgaggt tccttggatt
       61 gctagtgcag ctagaactgc taaaccaaat gagaccccta ggcttgtctc ttcaataagc
      121 aagctgagaa caaagaaaaa accagtgaat gcgaacatgc cataaagcaa cccgcgcaag
      181 acgtgagccg cagcggcggg tccgtaatca cgatgggcaa aaatgcttag gatggtgaca
      241 taaagtggga tggtggtgag gagcccggtc aggcgggaac cgataaatgg ggcaataccg
      301 gtgagtagca aaataaagct tgtgccaatc agaattcgca cgggaatatc ccactggctc
      361 aatttgcttt ctcccggctc tacctctcct ttgggcatgt aagttaatcc aattaagatg
      421 gctgctacaa ccacaggcac aatgaaccaa agagaaacag ttatgttttg taaaaataaa
      481 gtactcaacg aaaaaacaat gaagctccct acaaacgcga cataccaagg gaaacgtgtg
      541 gcaacccatg catatgttag ggcatacaac accagcgaaa ttccgccact gagaacacct
      601 aaaactgaac tttctgcaaa ggtcgcacca tggcttaacg ccacaaaaaa taccaccgga
      661 cctgatgtaa gtggcatccc tactatccaa ccgctaacgg caggtcccca ccggcgacct
      721 gccaaactgg cagacccaat aatgagaggc gcaagaatta gttttagagc aagaacattc
      781 attagggatt caccagaaag aatatagcta acaagatcga aagatctgca atagaatggc
      841 tcacaacagg tgtgagtaaa cttccgtctt tgcgatacag ctgacgccaa agccagccaa
      901 taaaggtcaa tgcaagaagt acaagcacaa ttgatagcgg cggagacgtt ttgttccaca
      961 caatcatagt gtgataacct gcatagacca gatcgcccaa gtaaaacgat tttatgtcgc
     1021 tccctaaaac gatacgccaa aaaaattctt ccataaaggg gttgataatc gaaaagtata
     1081 aaatgaacca ttcccaaaca ttgccctgta aaccaagagc cgtgagttgc acgcgaaggt
     1141 tcggagaaat ccctaaaaaa tcacgcagta gatataggct aggtccactt agagcacaga
     1201 gtaatatagt aaagaaaaat aattttttac tcgtgctctg aaaaaaagta cgaaaagagt
     1261 attggggttt tagtaaaaat atgacaatca gaattgcgat atgaaaacca agtaaagccg
     1321 cccaggcatt ttttaaaacg aacatcccca cccaaacagc aatatagggt gggatagcta
     1381 aaaacaattt tgatttcata ggaggattac ttaacttcgg tgatgactgt ccgttttgcg
     1441 cctgcggaca tgagtgctgc cgctaccaac tccgctgagt cttgattcac caacgcaatc
     1501 atattgcccc cgcgtccgcc accgctgagc ttggcgccaa gtgcccctgc tttacgggct
     1561 gcatccacaa gcctatccag ttcaggcgaa gagacggtca ttttttggag aaactcgtga
     1621 ttgtggtcca tcaactcacc gagatgctcg ggacgtccgc tttcgatgaa gtgacgtgcc
     1681 atggtggaga tctgcgctac ttcgttgaag atgttctcga agtggtttgt atccctttgc
     1741 catagacggc gcacatcccc tacggattct ttggtgggcg caggaacccc tgtatcgcca
     1801 atcacaatgg tgaagggttt gccgcatttg aaggtttcaa tcggcttgcc tttcatataa
     1861 tacacaggca tattataggt aatgaccgta ttatctatgc ctgagggcgt gccatggtga
     1921 agtttttcaa tttcaaaaac aagttcattg acttgctcat tggaaagcga atggtttagg
     1981 aaagaagata atgccctaat taaagcaacg gaaactgcgg caccagagcc gagacctgca
     2041 gcaacaggga tggtagagta gatggttagt tttaggtcag gctgtgggga gatgcctaag
     2101 tgttgaaaca ctttcagaat caccgaacca attggatggt cggaaggaag cgtggaaagc
     2161 tcgccatgca agttcaccat cggggcattg atccaaacgc caggcttgtc tgagtctaat
     2221 acttccacat ccacttgcac ttggttgaca gggacggcaa gcgcaggacg attgtataca
     2281 acggcatgtt cgccgaagag aatgatcttg ccgggagcgg aagatttcat gaaaagtaaa
     2341 agatgataat gacggtcact gcccaaagca gcatggagat ttggaaggga cgatcagaaa
     2401 gcaaaacttc ctctggcgtg ccaccggcat gttctacttc gatgagataa agatagcgga
     2461 agatggcata caccagaaat gggatagtga gcatcatgga gtgattttcg ggcacattgg
     2521 gtgctgagaa ggtatagagt gaatatgcca caatggttgt gccggagacg atcatgatgt
     2581 attggtcgag taagggcaaa gtatagccat cgagcactt
//
LOCUS       DAOQJE010000533         2606 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_28262_length_2606_cov_5.942768, whole genome shotgun sequence.
ACCESSION   DAOQJE010000533 DAOQJE010000000
VERSION     DAOQJE010000533.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2606)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2606)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2606
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28262_length_2606_cov_5.942768"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            151..1728
                     /locus_tag="PLT08_12990"
     CDS             151..1728
                     /locus_tag="PLT08_12990"
                     /inference="COORDINATES: protein motif:HMM:NF012602.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HNE05434.1"
                     /translation="MSNPLSDMEKADVYFCIGTNMTECHPVAATRMKKALARGSKLIV
                     ADPRYIKLAEMSDLYLPLRVGTDVALLLGMAHVIVRENLLDHKFIEERTTGIDEYIAH
                     VKQFTPEWASEITGVPAKDIEKAAILYASADKASIYYTLGITEHICGVDNVQSLSNLA
                     LMTGHLGREGCGINPLRGQNNIQGAGDAGTAPSDYPGYQSVLKPEMREKFSKAYGREM
                     DAEKGITKVTALELCGEGIHAMLIDGENTVVSDPDRAHSERALGKLDFLCVIDIFLTE
                     TAAMADVVLPATSWGETDGTFASTERRIQRVRAAVTPPGEAKPDWWILSALANTMGIQ
                     GFEYDSPAEVFNEICSLAPIYAGVDWDLIDGGQYQWPIPYKGHPGTPRLHEDGFTNGR
                     AVLRAIGYRDPAEVVDDEFPVWLTTGRRLQNYHTRTQTGRASGIEYLAPEEYLEVHPD
                     NVQKWGLTDGGWAKVTSRRGEIKIKVKSTPRSPRGTVFASFSYADTPVNLLTGSGYDP
                     TTHTAEMKVTVVRVEPL"
     gene            1810..>2606
                     /gene="fdhD"
                     /locus_tag="PLT08_12995"
     CDS             1810..>2606
                     /gene="fdhD"
                     /locus_tag="PLT08_12995"
                     /inference="COORDINATES: protein motif:HMM:NF001943.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory
                     sulfurtransferase FdhD"
                     /protein_id="HNE05435.1"
                     /translation="MTRFNKKKQTIWTYENGKPQRRKDILATEEPMEILLRAGETQQT
                     VAITMRTPGNDYELAAGFLYNEGILLRKDEITGMTYCVDGNQQEYNSLRIQLKADSMP
                     ELKQLERHFFTNSACGVCGTTMLDDIGERNLPALPHGPIVSPDLITSLQDKLRSAQDL
                     FESTGGLHAAALFDLEGNLLAVREDVGRHNALDKLIGWGLLNDQLPFHNKVLMVSGRA
                     SYELLQKSYSSGISVFCAVSAPSSLAVELAERFGITLIGFLRGQRFNV"
BASE COUNT          633 a          740 c          677 g          556 t
ORIGIN      
        1 gtctccagcg gacgcactcc caccgaagcc gattacctga tgcagaaatt catccgtgcg
       61 ggtttcggca cccataacat cgataactgt agccgtgcct gacacgcgcc cactgtcgcc
      121 ggtctggcgg caaccattgg tcgcggtgcc atgtcgaatc ccttatctga catggaaaag
      181 gcagatgtct atttctgcat tggcacaaac atgacggaat gccaccctgt ggcggcaacc
      241 cgtatgaaga aagcattggc tcgcggatcc aaactgatcg tcgcggatcc gcgttacatt
      301 aaactggcag agatgtctga cctctacctg cccctgcgcg tcggtacgga tgtggcgtta
      361 ttgctcggca tggcgcatgt catcgtccgc gagaatttgc tcgaccataa attcatcgaa
      421 gaacgcacca cgggtattga tgaatacatt gcgcacgtca aacagttcac acccgaatgg
      481 gcttctgaga tcacaggcgt acctgccaag gatattgaaa aagccgccat cctgtatgcg
      541 agtgccgaca aagcatccat ctattacaca ctcggcatca ccgaacacat ctgcggcgtg
      601 gataacgtcc aaagcctgag caatctcgct ctgatgacag gtcatttggg acgcgaaggc
      661 tgcggcatca atccgctgcg tgggcagaac aacattcagg gtgcgggtga tgcgggaacc
      721 gctccgagtg actatcctgg gtatcaatcc gtgctcaagc cggagatgcg tgaaaagttc
      781 tcgaaggcat atggtcgcga aatggatgct gagaagggca tcaccaaagt gaccgcgctc
      841 gaattatgcg gtgaaggtat tcacgctatg ttgatcgatg gcgagaatac cgtggtttct
      901 gacccagacc gtgctcacag cgaacgcgct cttggaaagt tggatttcct gtgcgtgatc
      961 gatatcttcc tgaccgaaac tgctgccatg gcagatgtgg ttttgcctgc tacctcatgg
     1021 ggcgaaacgg acggaacctt tgccagtact gagcgccgca ttcaacgcgt gcgtgcagcg
     1081 gtcactccgc cgggagaagc caaacccgat tggtggatct tgagcgcgct cgccaatacc
     1141 atgggcattc aaggctttga atacgactca cccgccgaag tcttcaatga gatttgcagt
     1201 ctcgcgccca tctatgccgg tgtggactgg gacttgatcg atggtggtca ataccagtgg
     1261 cccattcctt ataagggtca ccccggcacg ccgcgtttgc atgaagatgg cttcaccaat
     1321 ggacgtgccg ttttgcgagc cattggctat cgtgaccccg ccgaagttgt ggatgatgaa
     1381 ttccccgtct ggctcactac gggtcgccgt ttgcaaaact atcacacccg cacccaaacc
     1441 ggtcgtgctt caggcattga ataccttgcg ccggaagaat atttggaagt acatccagac
     1501 aacgtccaga agtgggggct gacggatggc ggctgggcaa aggtcacgag ccgacgcggt
     1561 gagatcaaga tcaaggtcaa atccacaccg cgctctccgc gtggaacggt cttcgccagc
     1621 ttcagctatg cagatacgcc cgtcaacctc ctcacaggct ccgggtacga ccccaccacc
     1681 cacaccgctg aaatgaaagt gaccgtggta cgagtggaac cgctgtaaga aactcaacgc
     1741 ccaaaaagtc aaaacggaga ccggcgaaac ttcccggtct ccgttcgctt tattttagag
     1801 aaggtgcgca tgacacgctt taataagaaa aaacaaacca tttggaccta tgaaaacggc
     1861 aaaccacaac gccgcaaaga tatcctcgcc accgaagagc cgatggagat tcttttgcgc
     1921 gccggggaaa cccaacaaac ggttgccatc accatgcgca ctcccggcaa tgactacgaa
     1981 ctcgcggcgg gctttctcta caacgaaggg attttgctcc gcaaagatga aattaccggc
     2041 atgacctatt gcgtagacgg gaaccaacag gaatacaact cgcttcggat tcagctcaaa
     2101 gctgattcca tgcccgaact taagcaactc gaacggcatt tcttcaccaa cagcgcctgc
     2161 ggcgtttgcg gcaccaccat gctcgatgat attggcgaac gcaatttacc agccctccct
     2221 catggaccca tcgtctcgcc tgatctcatc acgtccctgc aagacaaact tcgcagcgcg
     2281 caagatttgt ttgaatccac aggcggactt cacgccgcgg cattattcga cctcgaaggc
     2341 aatctgctcg ccgtgcgcga agatgtgggg cgtcacaacg cgttggataa attgatcggc
     2401 tgggggttgc tcaacgatca gcttccattc cataacaaag tcttaatggt cagcggacgt
     2461 gccagttatg aactgctgca aaaaagttat tcatcgggca tctcggtctt ttgcgccgta
     2521 tccgcaccca gcagtctggc agtggaactt gccgaacgct ttggcattac gctcatcggc
     2581 ttcctgcgcg gacaacgctt caatgt
//
LOCUS       DAOQJE010000534         2599 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_28403_length_2599_cov_3.397013, whole genome shotgun sequence.
ACCESSION   DAOQJE010000534 DAOQJE010000000
VERSION     DAOQJE010000534.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2599)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2599)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2599
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28403_length_2599_cov_3.397013"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(111..794)
                     /locus_tag="PLT08_13000"
     CDS             complement(111..794)
                     /locus_tag="PLT08_13000"
                     /inference="COORDINATES: protein motif:HMM:NF016085.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF402 domain-containing protein"
                     /protein_id="HNE05436.1"
                     /translation="MAGWSLIGRNNLNPKSYRLAGENATLRGVGIKVFWAYPTIVVED
                     TSELIALYMPAGVLGKDTAHKPTPEEILAAEEIVIADYQWKRTDVLFLIVPGEAFSTY
                     AMWKTGTKNLDCWYVNLQEPIRRTPIGFDTMDHMLDVVIEPDMNKWRWKDDDEFSKAQ
                     EAGIYSKEIASIIWQAGEKAVRLITSERRSFYEKWATWQASPEWSLPKLSPHWDKVNF
                     DGLDLQNKV"
     gene            complement(938..1492)
                     /locus_tag="PLT08_13005"
     CDS             complement(938..1492)
                     /locus_tag="PLT08_13005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE05437.1"
                     /translation="MDEAFISIMVSVVGLLSGVIGATIAARYQLKLEQEKWRAMRQDS
                     LEKETRLAVAELCKKMVEVVQEIGWLSWSATYQPNRITIKHAKEYDLKIKQLLPQVMS
                     SLVVVSSLDSSVHNTMDMLVKKVYALDNNMSKALIVFEKSRKNGIEVLKTCHPDVKEF
                     WEILNIAVADQFNGKISKSRKDIS"
     gene            complement(1728..2513)
                     /locus_tag="PLT08_13010"
     CDS             complement(1728..2513)
                     /locus_tag="PLT08_13010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE05438.1"
                     /translation="MNKRFEGIAIILAVIGVFIALLAWLAPFSPIGTSPLAGQNANNT
                     IPIETVIVPANSSNIVGSDTVLYDDFNDNAYDGKYNLEKWIIDMNGGKIYQQDGALVA
                     QVDSSLGDGISLSAVDYQNIKIETPMFFEAKLMIKEPINGHVFLYLGSSSSSGIFSDC
                     TLGYGDVIALLDCGYVVNGVTEYRQDDELVNFTTWHTVRIEVEPQTMTLTYYLDGQKI
                     HSFIPSNSAYLKSGNFTLQIGIVSDDKKSWTGFIDDVSIGQIP"
BASE COUNT          707 a          579 c          492 g          821 t
ORIGIN      
        1 ggacggcgaa gccgtccagc cagaaaaaag ctaaggcgta ggaaactgct tgggacgacc
       61 gcagaatccc cagcgtcagg tgcacgcttt gttggctggc gtggacttgc ctaaaccttg
      121 ttttgcaaat caagcccatc aaaatttact ttgtcccaat gcggagataa ttttggaaga
      181 ctccattcgg gacttgcctg ccaagtagcc catttttcat aaaacgaacg ccgttcagat
      241 gtaatcaatc ttactgcttt ttcacctgct tgccaaataa tactggctat ttcttttgaa
      301 taaatgcctg cttcctgtgc ttttgaaaat tcgtcatcat ctttccagcg ccatttattc
      361 atatctggtt cgattacgac atcgagcata tgatccatcg tatcaaaacc aataggtgta
      421 cgcctgatag gttcttgtaa attgacgtac caacaatcta gattctttgt acctgttttc
      481 cacattgcat aagtgctaaa tgcttcgcca ggaacaatga gaaataaaac atcggttcgt
      541 ttccattgat aatcagctat aactatttct tcggctgcca atatttcttc tggggtaggt
      601 ttgtgggcag tatcttttcc aagaacgcca gcgggcatgt acaaagcaat taattcggat
      661 gtgtcttcaa caacaatagt aggataagcc caaaagactt tgatgccaac gccgcgcaaa
      721 gtcgcatttt cacccgccaa ccgatacgat tttggattca aattatttct cccaattaat
      781 gaccagccag ccaacggatt gcgttacctg ccccgtcgcg tggacttggc tcatccgtcc
      841 agccagaaaa aggctaaagc ggagtcttcc cctttatggg aagatgtcca aaggacggaa
      901 gaggtcaggc acgcgctttg gtgggtgttc tttgttttta cgaaatatct tttcttgact
      961 tacttatttt tccattaaat tggtcagcaa cggcaatatt taatatttcc caaaactctt
     1021 ttacatctgg atggcaagtt ttcaggactt caattccatt tttgcgagat ttttcaaaga
     1081 cgataagtgc ttttgacata ttgttgtcta atgcatacac tttttttacc aacatatcca
     1141 tcgtattatg aacactgcta tctaaagaag ataccaccac tagcgaactc ataacctggg
     1201 gcagtagttg ctttattttc agatcatatt cttttgcgtg tttgatggtt attctatttg
     1261 gctgataagt tgctgaccat gaaagccagc caatttcctg aactacttca accattttct
     1321 tgcacaactc tgcaactgct aatctggtct ccttttcaag agaatcttgg cgcattgctc
     1381 gccatttttc ttgttcaagt ttaagctggt atctcgccgc tatagttgcc cctataacac
     1441 ctgaaagcag cccaactaca gagaccatta ttgatataaa ggcttcatcc atatatttat
     1501 ttgccttaag ttatttgatt tttggacgta ccgcccaacg gctttacatt acctgccccg
     1561 tttcgtggac ctggctcatc cgtccagcca gaaaaaggct tagcaaatcg acctgccccg
     1621 taggtacatt ttacatctca acccaactta aaatgtcgta tttggggagg gtgccgaagg
     1681 cgggtggggc aggtgcacgc tttgttagcc cgcgttgcta ttaaacgtta tggaatttgt
     1741 ccaatactta catcatctat aaaacctgtc catgattttt tatcgtcact gactattcca
     1801 atttgtagcg tgaaattacc agactttaag tatgcagagt tgctggggat aaatgaatga
     1861 attttttgtc cgtctaaata gtaggttagc gtcatcgttt gtggctctac ttcaattcta
     1921 actgtatgcc atgttgtaaa gttgactaat tcatcatctt gtctgtactc tgtgacgcca
     1981 ttaactacat aaccacaatc taataaagca atcacatcgc catagcctaa cgtgcaatca
     2041 gaaaatatcc ctgaagatga acttgagccg agatataaaa aaacgtgacc attgataggc
     2101 tctttaatca tcaactttgc ttcaaaaaac attggcgttt ctatttttat gttttgatag
     2161 tcgacggcgc ttagactgat accgtctcct aacgaactat ccacttgggc aacaagcgca
     2221 ccatcttgtt gataaatttt cccgccattc atatctatta tccatttttc aaggttgtat
     2281 tttccatcgt aagcattgtc attaaagtcg tcataaagta cggtatctga gccaactata
     2341 ttgcttgaat tagccggtac tataacggtt tcaataggta ttgtattatt cgcattttgc
     2401 cctgctaatg gtgatgtccc aatagggcta aatggggcaa gccaagccaa aagcgcaatg
     2461 aaaacaccaa ttacggcaag aataattgca atgccttcaa atcgtttgtt catttctcct
     2521 gcctttctgc ttgaagattt cttaaaatat gaaaactgtg gtatatgcaa atatttttgc
     2581 tactagcggg ctaacggtt
//
LOCUS       DAOQJE010000535         2598 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_28415_length_2598_cov_6.184035, whole genome shotgun sequence.
ACCESSION   DAOQJE010000535 DAOQJE010000000
VERSION     DAOQJE010000535.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2598)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2598)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2598
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28415_length_2598_cov_6.184035"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..807
                     /locus_tag="PLT08_13015"
     CDS             <1..807
                     /locus_tag="PLT08_13015"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HNE05439.1"
                     /translation="WNPDALIMEANPRYLASPSAVNWMHKRGRKVLGWGLGAPKQSGL
                     WKRFIHQFDGMIAYSQRGAEEYAALGFPREKIFVAHNSVSAKPEKPDDRPLTIDHVTI
                     LFVGRLQARKRIDSLLRACAEMDTKPRLLIIGDGPERETLQTLAKEIYPSAEFIGAKH
                     GAELKPYFQQADLFVLPGTGGLAVQEAMSYGLPVIVAKGDGTQDDLVREKNGWQIEPE
                     NYEALVSTMKNALSDIARLREMGKESFRIVSEEINIEKMADTFVRALNSL"
     gene            complement(847..1281)
                     /locus_tag="PLT08_13020"
     CDS             complement(847..1281)
                     /locus_tag="PLT08_13020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE05440.1"
                     /translation="MHKYDEFVHKELAPHLSAGEGIELTGFLYTKSLRAVVLTGGLSL
                     VGDGYYFAALTRRRMFIIATTMGVVSLNMENKGMSEILYTDIKSIKPSGFLNQKMLAI
                     ELKDGKTLVFRLNTLATNFASGQKQFIETLIEFVGSGSPRSN"
     gene            complement(1281..1757)
                     /locus_tag="PLT08_13025"
     CDS             complement(1281..1757)
                     /locus_tag="PLT08_13025"
                     /inference="COORDINATES: protein motif:HMM:NF021378.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SHOCT domain-containing protein"
                     /protein_id="HNE05441.1"
                     /translation="MKRPNWMVVGILFGVSLFLGYAIFTALTTALFPAVNNVSGKFLC
                     DGEYKIVTTQSSPRPGQTYWSHTILCDGKDITLPAVAITGLIASTVFFVLLLILTRNW
                     WFREKAALAPFANAGLPAKGKKSALERMAELKEMRDKNLISQVEYERKKDDIMKEL"
     gene            complement(1766..2509)
                     /gene="budA"
                     /locus_tag="PLT08_13030"
     CDS             complement(1766..2509)
                     /gene="budA"
                     /locus_tag="PLT08_13030"
                     /EC_number="4.1.1.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR01252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047605 - acetolactate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA];
                     GO_process: GO:0045151 - acetoin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate decarboxylase"
                     /protein_id="HNE05442.1"
                     /translation="MERTTIRRSRKRRADSKIYLCAPVNSLVEGIYEQNIPFHEIRKH
                     GDMGLGTFNDLDGEMMMVDGEIYRVGSDGKVNLITDDDTCTPFACVTFFQPASDEVLE
                     KEMDYEQFLRWMMELMPSPNIFYAFRITGEFAYVKTRSVPKQENYRPLVEVAKEQPTF
                     EFHNVSGKMVGFYTPSFVSALSVPGVHLHFLTEDKTSGGHLLACHPRKVKVELQYLYT
                     VELSLPANLDYLTWDFQRDIGKDLDKAEK"
BASE COUNT          671 a          636 c          620 g          671 t
ORIGIN      
        1 tggaatcccg acgcgctcat catggaagcc aatccgcggt atctcgcctc tccctccgcc
       61 gtgaattgga tgcacaaacg cggacgaaaa gtcctcggct ggggcttggg tgctcccaag
      121 caaagtggac tctggaaacg tttcatccat caattcgacg ggatgatcgc ctacagtcaa
      181 cgcggcgcgg aggagtatgc tgcgctaggt ttcccgcgtg agaagatttt tgtggcgcat
      241 aactcggtgt cagcgaaacc ggaaaaacct gacgacagac cattgacgat tgaccatgtg
      301 actattttgt ttgttgggcg gttgcaagcg cgcaagcgta ttgattcatt gttacgcgcc
      361 tgtgctgaga tggacactaa gcctcgtttg cttattatcg gcgatggacc cgaacgcgaa
      421 acgttgcaaa cattagctaa agaaatttat ccatccgctg aattcatcgg tgcgaagcat
      481 ggtgcagagt tgaagccgta ttttcagcaa gcggatctgt tcgtcctacc cggcacaggt
      541 gggcttgccg tacaggaagc catgagttac ggtctgcccg tcattgtcgc caaaggcgat
      601 gggacgcaag atgatctagt ccgcgagaag aacggctggc agatcgagcc ggagaattac
      661 gaggcattgg tctctactat gaaaaacgcg ctatctgata tagcgcgtct gcgagagatg
      721 ggaaaagagt cgtttcggat cgtttctgaa gagatcaata tcgagaaaat ggcggatacg
      781 ttcgtaaggg cattgaattc gttgtaatcg taggggcgcg gtttccgcgc ccttgatgtt
      841 tccctattaa ttggagcggg gagaccccga ccctacaaat tcaatcaacg tctcaataaa
      901 ctgtttttgc cctgaggcaa aattcgttgc cagtgtattc aagcggaaga caagggtctt
      961 gccatctttg agctcaatgg cgagcatttt ctggttgagg aatccgctcg gcttgatgga
     1021 cttaatatca gtgtagagga tttcgctcat gcctttattt tccatgttga gcgaaacgac
     1081 gcccatcgtt gtggcgatga tgaacatgcg tctgcgagtg agggcggcga agtagtatcc
     1141 atcaccgacc aagctcaaac cgccggtaag gacaacggct ctcaaagatt tggtgtagag
     1201 aaagccggtg agttcgatcc cttctccggc ggagagatga ggcgctagtt ccttatgaac
     1261 aaattcatca tatttatgca tcacagttcc ttcatgatgt catctttttt ccgttcatac
     1321 tccacctgag agatcaagtt cttatcacgc atttccttga gttctgccat ccgttccagt
     1381 gcagatttct tccctttcgc tggcaagcca gcatttgcaa atggagctag agcagccttt
     1441 tcacgaaacc accaattgcg tgtgaggatg agcaacaata caaaaaagac agtagatgct
     1501 atcaaacccg ttatcgcgac agcgggaagc gtgatgtctt ttccatcaca gaggatggta
     1561 tgagaccaat aagtttgccc ggggcgcgga gaagattgcg tggtgacgat cttatattcg
     1621 ccatcacaca aaaatttacc tgaaacattg tttacagccg ggaagagcgc agttgtcaac
     1681 gctgtgaaaa tggcatatcc taaaaacaaa cttacgccaa acaaaattcc aacaaccatc
     1741 cagtttggtc ttttcatctc aacctctatt tttctgcttt gtccaagtct ttaccaatat
     1801 cacgctggaa atcccaagtg agataatcaa gatttgcagg caatgaaagt tctaccgtat
     1861 aaagatattg caactccacc ttcactttac gtggatgaca tgccagcaaa tgtccaccgc
     1921 tggttttatc ttcggtcaag aagtgcaaat gcacgcccgg cacactcagc gcagagacga
     1981 aagacggcgt ataaaaccca accattttgc cgctcacatt atgaaattca aaagtcggtt
     2041 gttccttcgc cacttccacc agtggacggt agttctcctg cttcggcacc gagcgcgttt
     2101 tgacatacgc aaattcaccc gtgatgcgaa aggcataaaa aatattcggc gatggcatca
     2161 actccatcat ccaacgcagg aactgctcgt aatccatttc tttttcgagc acttcgtcac
     2221 tagcgggttg aaagaaggtc acacaggcaa atggcgtaca agtatcatca tctgtaatga
     2281 gattgacctt gccatcagac cccacacgat aaatttcgcc gtccaccatc atcatctcgc
     2341 catcgaggtc attgaacgtt cccaagccca tgtcgccatg tttgcgaatt tcatggaaag
     2401 ggatgttctg ttcataaatg ccttcgacga gtgaattgac cggcgcacat aaatagattt
     2461 tgctatctgc gcgcctcttc ctggacctgc gtatggttgt tcgttccatt tgaatgtatt
     2521 ctcctctata agctgttccg tacaagatca ttcgtatgtt ggcgtatcgc cgataatttc
     2581 ccaaccgcgt tcaagtgc
//
LOCUS       DAOQJE010000536         2597 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_28425_length_2597_cov_5.122345, whole genome shotgun sequence.
ACCESSION   DAOQJE010000536 DAOQJE010000000
VERSION     DAOQJE010000536.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2597)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2597)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2597
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28425_length_2597_cov_5.122345"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            119..331
                     /locus_tag="PLT08_13035"
     CDS             119..331
                     /locus_tag="PLT08_13035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE05443.1"
                     /translation="MEDWLTTYEAVRLSGYELDYIRKLVRAKKIVGRKWGQSWQVSRS
                     SLLEFLKTREKEGQKRGRKPLSRDDK"
     gene            597..836
                     /locus_tag="PLT08_13040"
     CDS             597..836
                     /locus_tag="PLT08_13040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE05444.1"
                     /translation="MLQTPSTQRFQIVGSLARIRQEWQDAAGTSSLIEVEGNMGMLLA
                     DLINGLDLGTNEQVQVLGQELFQELKDFLKSPVQN"
     gene            919..1722
                     /locus_tag="PLT08_13045"
                     /pseudo
     CDS             919..1722
                     /locus_tag="PLT08_13045"
                     /inference="COORDINATES: protein motif:HMM:NF012464.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
     gene            complement(2504..2554)
                     /locus_tag="PLT08_13050"
     CDS             complement(2504..2554)
                     /locus_tag="PLT08_13050"
                     /inference="COORDINATES: protein motif:HMM:NF012488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE05445.1"
                     /translation="MQKLDADFLVSSSYKY"
BASE COUNT          737 a          535 c          658 g          667 t
ORIGIN      
        1 acagactata aatcctggta ggaaccttac catcgccata acaccagtca atgtttcatc
       61 ctgatcgaat ttatatgctg cctgcgcggt tataatttca catggagacc cgtttcaaat
      121 ggaagattgg ttgaccacat atgaagccgt ccgcctcagc ggatatgaat tggattacat
      181 ccgaaaattg gtacgcgcca agaagattgt ggggcgtaag tggggacaat cctggcaggt
      241 cagccgatcc tctcttttgg aattcctgaa gaccagggaa aaagagggac agaaacgtgg
      301 tcgcaaaccc ctttcgcgtg acgacaagta acagtttgta aatctgtaat ctgatactta
      361 gaataaatgt gctataataa tttcattgct ggcaatgaaa ttattagcca gagaaacggg
      421 ccgcactggt gttagagcac cgatacgacc cttacccaac ccaccggtag tagcggaggg
      481 aagggctggt ttgattatac agcccctttg attcgccttg gtgggattcc atcagggcga
      541 tttgcgtttc tgccgggaga agcagaccgc ccaatcaaaa tcaaaaggag atgtatatgc
      601 ttcaaacccc ttcaacacaa cgctttcaga tcgttggatc tctcgcccgt atccgccagg
      661 aatggcagga tgccgcaggg acatccagtt tgatcgaggt ggagggcaac atgggaatgt
      721 tgcttgctga cttgatcaat ggactcgacc ttggaacaaa cgaacaggta caggtactgg
      781 gtcaggaatt gttccaggaa ttgaaggatt ttctcaagtc ccctgttcag aactgatgca
      841 tacaagccag ccttaccggc gcggctggct tgtatcttcc atattgtttt acaacgatca
      901 aaaaagaggt ttcttacaat gaatgataga gcagttacat atgcgcgtgt ctcaggagat
      961 gatcgcgtta aggatggtcg aaacctgtcc ggtcagcttg atatttgccg tgaacatgca
     1021 ctcaaaaaag gttatcaaat agtcgcagaa ttatccgagg atgatcgcgg agcatctggc
     1081 gtttcatttg aacttccagc cttgaatcag gcattggaga tggcaaagaa tggtgaattc
     1141 gatatcttgg tggttcgtga gatcgaccgt tttgcccgaa gtcttgccaa acaactgatc
     1201 atcgaacagg aatttcgtcg ttatggagtt cagatcgagt atgtcatggg tgaataccca
     1261 gatacacccg aagggagtct aaacaagaat atcaaggcgg tcattgctga atatgaaaga
     1321 ttgaagatca atgaacgtat ggttcgtggt cggcggcaga agatcaaagc tgggaatgtg
     1381 atcattcatg gaaagcctcc ctatggatac aggcttgaag aagttgatgg caaaagtaag
     1441 ttgctggtca atgaagacga agcccagatc gtgaggatga tttatcaatg gtatgttttt
     1501 ggcgaggata aaaaaggacc aatgtctgca cgagttattg cccgtcgtct tactcaaatg
     1561 ggcgttccca tccctccttt ctccatccga agttcttcta aatggtcgcg tactacagta
     1621 gggcgtatcg tcagcagtaa gacctacatt ggtgtctggc agtatggaaa aaatggcagt
     1681 tcgaatgggc gtcgatttat taatcccgag gatcactggt tgtcagtcga agttcccgca
     1741 atcgtagatt tggatgtgtg ggatgctgct caagatagga aagcgatcaa catcgccagt
     1801 gcgaagaaaa acctgaaata tcaatacttg atgaatcgga ggttgatttg tggtgcctgt
     1861 ggttccaaga tttctgcctg cggcagtaat gatggatata agaatcgcct atattatcga
     1921 tgtaatgttg cccgaggtta cgatgaatac gagcgcaaat gtggaaatgc acgatacttc
     1981 aaatctgaag atgtagactt taccatctgg gaatggctga aatcctttat cagaaaccca
     2041 gatgccatag atgcgggtat agctgaatac catgaaaacc gggagcgtga acaggcaccc
     2101 attcgggaac gccttcaggt tgtagacgat ctgctgattg atcaccgtag tcaattgcaa
     2161 aaattacttg acctttacct ggatggaaat ttccccaagg aattacttgt cgagagacgc
     2221 cagagattgc aggatactgt tgactctctt gcaaatgaac gggtcaatct tgtatcccac
     2281 ctggagaata gtgcctattc gccggaacaa ttgcaagcta tgaaagaata cactcaggaa
     2341 atcgctgctg ggctggacga agcggaagaa gattttgagt ccaggaaacg tatcgttgag
     2401 atgttgaatg tttatgcttc tttggctgta gaaggtgatg agaaagtagc gtatgttact
     2461 tgcgtattca aaactgaccc cgaacgcttg ttgattgtgt cgcctaatat ttataagaag
     2521 aagacaccag aaaatcagcg tcgagctttt gcacgtcaat cgcgccgtgg acggcgtatt
     2581 gcaccgcatc tacatac
//
LOCUS       DAOQJE010000537         2595 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_28478_length_2595_cov_2.923228, whole genome shotgun sequence.
ACCESSION   DAOQJE010000537 DAOQJE010000000
VERSION     DAOQJE010000537.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2595)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2595)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2595
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28478_length_2595_cov_2.923228"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1371
                     /locus_tag="PLT08_13055"
     CDS             <1..1371
                     /locus_tag="PLT08_13055"
                     /inference="COORDINATES: protein motif:HMM:NF024883.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HNE05446.1"
                     /translation="FTLVLLASGAWVTRQSVLKPLSELGVAAEHLGGNDLETPVQVQG
                     PEEMQRLSQSFDGMRLGLKSAQQDLIDLNDTLEKRVSQRTRELEALNIVSQEITSRLD
                     IQQVLSSVTKKTSELLNGDVSFLCLLDANQPLLKFQTFSGHADAAVDDTTVIKKHDLT
                     DSILNSSKAVVCGIDTCREGCSILSNKYRSSHMAAPLRSNDQIIGALCVGSTSTQRFG
                     GESTDLLTKLANTAAIALENARLYEQAERIATLEERRRIAADMHDGLGQTFSYLGLMT
                     DQVVELLSNGKNQAALERLHLTRETIEKATGNVRKAINRLMDENPINSDLCDQLQGVA
                     KELSVQHNAIIEWQCETTSPCNCPPLVNEQVIHIVREALTNAISHAHPTQIIVQFTKR
                     DHAYELLIADNGKGFDSSQPSPNGHFGLQIMKARALQINGQLEVKSMPGMGTQIILSF
                     PLSNKE"
     gene            1379..2041
                     /locus_tag="PLT08_13060"
     CDS             1379..2041
                     /locus_tag="PLT08_13060"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HNE05447.1"
                     /translation="MKKIRVMLVDDHALFREGLAVIIGSQPDMEVVGNANDGLEAVIK
                     AQELKPELILMDVQMSTMDGLEATRQIKQLLPDTTIVVLTVRDDDEKLFEALKSGAQG
                     YLLKDIRSHIMVEMLHSALNGEAAITPSLAGRVLAEFRRMSLNQKSGTDLPNEDNSLT
                     ERELEVLSLVAKGASDRDVAETLTVSLNTVKTHIRNILSKLRVNSRREAAKLAQSKGL
                     LS"
     gene            2196..2348
                     /locus_tag="PLT08_13065"
     CDS             2196..2348
                     /locus_tag="PLT08_13065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE05448.1"
                     /translation="MPDKEKEEPQHFGQKIFDNIWLLFVLSLLISTLIYNVWGIIDLV
                     NVPPAP"
     gene            2372..>2595
                     /locus_tag="PLT08_13070"
     CDS             2372..>2595
                     /locus_tag="PLT08_13070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE05449.1"
                     /translation="MLAPEKNWWKPMGRMEKSWLTVAFVWCVFLTVMMPLWYFYGKQN
                     VPTETYRTSPADYGAKVNAFVEQYTVGEDIV"
BASE COUNT          741 a          520 c          648 g          686 t
ORIGIN      
        1 tttactttgg tattgctcgc cagtggggca tgggtgacac gccagtctgt attgaaaccg
       61 ttgtctgagt tgggagtggc agctgaacat ttgggcggga atgatcttga aacgcccgta
      121 caggtgcagg gaccggaaga aatgcagagg ttatctcaat catttgatgg gatgcgtctc
      181 gggttgaaat ctgcccagca ggatttgatt gacctgaacg atacgctgga gaaacgggtt
      241 tctcagcgga cacgggaatt ggaggcgctc aatatcgtca gccaggagat cacttcgcgc
      301 cttgatattc aacaggtatt aagttcggtt actaaaaaaa ccagcgaact tctgaatggg
      361 gatgtttcat tcttgtgttt attagatgct aatcagcctt tgcttaaatt ccaaacattt
      421 agcggacatg cagatgcagc agttgatgat acaaccgtta taaaaaaaca tgatttgaca
      481 gattctatcc ttaacagtag caaggctgta gtttgcggta ttgatacctg ccgcgaaggc
      541 tgctcgatcc tgtctaataa atatcgcagc agtcatatgg cagctccttt acgaagcaat
      601 gatcagatca ttggtgcatt atgcgtaggt agtaccagta cacaaagatt tggcggtgaa
      661 tccaccgatc tgctcacgaa acttgcaaat acagccgcaa ttgcccttga aaacgcccgt
      721 ttgtatgaac aggcagagcg aattgccaca ctggaggagc gccgtcgtat tgcggcagac
      781 atgcacgatg ggctcgggca gacttttagc tatcttgggt tgatgaccga tcaggtggtt
      841 gaattgctct ccaatggaaa gaatcaggca gccttggaaa gattgcattt gactcgcgag
      901 accatcgaaa aagctacagg aaatgtacgc aaagccatta atcggctgat ggatgaaaac
      961 ccgatcaact ctgatctctg tgaccaattg cagggtgtgg caaaggagct ttctgtccaa
     1021 cataacgcca tcattgaatg gcagtgtgaa accacatccc catgcaattg tccgcctctg
     1081 gtaaatgaac aagttattca tatagtacgt gaagcgttaa ctaatgcgat ttctcatgct
     1141 cacccaaccc agatcattgt gcaatttact aaaagagatc atgcttatga gttgctgatt
     1201 gcagacaacg gaaaagggtt tgactcttcc cagcccagcc cgaatggaca ttttggtttg
     1261 cagatcatga aggcaagagc tctgcaaatt aacggacaac ttgaagtcaa atccatgcct
     1321 ggaatgggga cacaaattat tctatcgttc cctttgagta ataaggaata aaattcagat
     1381 gaagaaaata cgagtaatgc tggtggacga tcacgccttg tttcgtgaag gactggcagt
     1441 cattatcggc tctcagcctg atatggaggt tgtaggcaat gcgaatgacg ggcttgaagc
     1501 ggtgatcaaa gcgcaggaac taaagccaga actgatcctg atggatgttc aaatgtcaac
     1561 tatggatggc ttggaagcga cacgccagat caagcagctt ctgcctgata cgaccattgt
     1621 ggtgctgacc gttcgcgatg atgacgaaaa attatttgag gcgttgaaaa gcggcgctca
     1681 gggatattta ttaaaggaca ttcgatctca tatcatggtg gaaatgctgc atagtgcctt
     1741 aaatggagaa gccgccatca caccgtcgct ggcagggcgg gttcttgccg agtttcggcg
     1801 catgagtttg aatcaaaaaa gtggtactga ccttccaaat gaagataact cattgactga
     1861 acgtgaactg gaagtgttgt ccctagtcgc aaagggcgca tccgataggg atgtggctga
     1921 gacgttaacg gtcagtttga atacggttaa aacacacatt cgcaatatcc tttccaagtt
     1981 gcgggttaat tcccgtagag aggcagccaa actggcgcaa agtaaggggc tgttgtcata
     2041 aaaaagaact aattcttttg ttaaaaagag tgatgtgaaa atcactcttt ttaaaattca
     2101 ctgattacat ataacaatac gattattggg gcgattaaag taacatgggt gctaagaact
     2161 caagtttgca tccatgtttc atgaggagaa tcgccatgcc tgataaagaa aaagaagaac
     2221 cgcaacactt cggtcaaaaa atttttgaca atatttggct gttgtttgtt ttgtccttgt
     2281 tgatcagtac cttgatctac aatgtgtggg gaattatcga tttggtcaat gtgccgcccg
     2341 ccccataaat cattgaattg gagaatggat tatgttagcc cctgaaaaaa attggtggaa
     2401 gcccatgggc cgtatggaaa aaagctggct cactgttgcg tttgtttggt gtgtctttct
     2461 tacggtaatg atgccgcttt ggtatttcta cggcaagcaa aatgtaccca ctgaaaccta
     2521 tagaacttcg cctgctgatt atggggcgaa ggtcaatgct tttgtggagc aatacactgt
     2581 cggcgaagat atagt
//
LOCUS       DAOQJE010000538         2584 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_28675_length_2584_cov_7.160142, whole genome shotgun sequence.
ACCESSION   DAOQJE010000538 DAOQJE010000000
VERSION     DAOQJE010000538.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2584)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2584)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2584
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28675_length_2584_cov_7.160142"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2508
                     /locus_tag="PLT08_13075"
     CDS             <1..2508
                     /locus_tag="PLT08_13075"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HNE05450.1"
                     /translation="DASDCLIFQVLDVTEKHNLAQSLVESNQRFAQLTRHIHEVFWVS
                     EEVGKLEYVSPAFEYVFGFPAEDLYQGKDYIDLVHPEDRQIVLDSFEYDMRGENSLFE
                     YRIIRPDGEIRWVSDRSYPILGEHGELIRTVGITTDITEQKHSEAERRELTSILEDAE
                     QQVGLASWSQDILTGKSKWSPYMFTLFGFDPAEGIPSMPDYLERIHPEDRELLGSALF
                     KMMQGEETSEMFTYRTNPHYGEMRFLKPTYRTEKDANGRVIKFLGTVLDITEQKRSAT
                     ELERRQKLLETVILAGKNITSIIDFDKCLQEIHRNIISNLGFERVGLFIYDPKRHYVQ
                     GTYGTDRNGNPVSNDWYYEQINENSEWQVALESPTGIAVIDDYGLARNPPPDNEMYGV
                     KQHITLAAWSGDTPVALISVDNLLTQKPITPADIEALQLFAGYAGLAIENARLHTGLE
                     NLIEKRTDELTQSQKSLQLFLDTANDLIQSLDESGRYIYVNQSWCRTLGFTLEEASKL
                     DMLQVVDKEYQDHCLNIFRNMMADGVSQMIEVGFRTKHGQLVIVEGSISIQTRSDRKR
                     VTNGFFRDVTERKQAEIAMRHANLEMEKALRMKDEFMANMSHELRTPLNAILGLSESL
                     LEETVGSINSRQHKYLNTISESGRHLLELINDILYLAKIESGQIHLASNSVNIKTLCE
                     ASLRIVDQLAKSKKQVMSLEVADEIKFVQADERRLKQMVVNLLSNAIKFTPENGQVGI
                     RALIDASRTFLNIEVWDTGIGIKADDLSHIFQPFIQLNSGLARESSGTGLGLALVAQM
                     ARLHGGSITVRSEIGKGSSFILSLPWTPELPDAPQEQ"
BASE COUNT          788 a          629 c          586 g          581 t
ORIGIN      
        1 gatgcatcag actgcctgat ctttcaggta ttggatgtca ccgaaaaaca caatctagcg
       61 caatctctgg tcgaaagcaa tcaacgtttt gcccaactta caaggcacat ccacgaagtg
      121 ttttgggtct ctgaagaagt aggcaaactg gaatacgtca gcccggcgtt tgaatatgtt
      181 tttgggttcc cagccgaaga cttgtaccaa ggaaaagatt acatcgactt ggtacacccg
      241 gaagaccgcc agatcgtact cgactcattt gagtatgata tgcgtggcga gaactcgcta
      301 ttcgaatacc gcatcatacg cccagacggt gaaattcgct gggtctcaga ccgttcctac
      361 ccgatccttg gggaacatgg cgaactcatt cgcacagtag gaatcacgac cgatatcaca
      421 gaacaaaaac actcagaagc cgagcgccgg gaactaacat ccattctgga agatgctgaa
      481 caacaagttg ggctggcaag ttggtctcaa gatattctga caggaaaaag caagtggagc
      541 ccctatatgt tcacactgtt cgggtttgac ccggcagagg gcattccaag catgccagat
      601 tatttggaac gcatccaccc cgaagatcga gaactgcttg gctccgcttt attcaagatg
      661 atgcaaggcg aagaaacgtc cgaaatgttt acctaccgca ccaatccaca ctatggagaa
      721 atgcggttct tgaaacccac ctatcgcacc gaaaaagatg cgaatgggcg cgtcataaaa
      781 ttccttggca ccgtgctgga tattacagaa caaaaacgca gtgcaacaga attggaacga
      841 aggcaaaaac tacttgaaac cgtcattctt gccggaaaga acatcacctc cattattgat
      901 ttcgacaaat gcttgcagga gattcaccga aacattatta gcaacctcgg gttcgaacgt
      961 gtaggcttat tcatctatga cccaaaaaga cattatgtac aaggcaccta cggcacagat
     1021 cgcaacggca acccagtcag taatgattgg tattacgaac aaataaacga aaatagcgaa
     1081 tggcaggtcg ctttggaaag cccaacaggc attgccgtga tcgacgatta tgggctagcc
     1141 cgcaacccac caccagataa tgagatgtat ggagttaagc agcacatcac tctagccgcc
     1201 tggtctggag atacgccagt ggctttgatc tctgtcgata acttattgac ccaaaagcct
     1261 attaccccgg cggatattga agccttgcaa ttgtttgcag ggtatgccgg gcttgccatc
     1321 gaaaacgctc gcctacatac cgggcttgaa aacctgatag aaaaacgaac cgatgaatta
     1381 acgcaaagcc aaaaaagtct gcaactgttt ttagacaccg ccaatgacct aattcaaagc
     1441 ctcgatgaaa gcggacgcta tatctatgtc aatcaatcct ggtgtcgaac attaggattc
     1501 acccttgaag aagccagcaa attagatatg ctccaagtag tggacaaaga atatcaagac
     1561 cactgtttga acatttttag aaatatgatg gcagatgggg tgtcgcagat gattgaggtc
     1621 ggctttcgca ccaaacatgg tcagcttgtg atcgtcgaag gcagtatcag cattcaaaca
     1681 cgaagcgaca gaaaacgggt caccaatggt ttcttccgtg atgtgaccga acgcaaacaa
     1741 gccgaaatcg ccatgcgaca cgccaacctc gaaatggaaa aagcattgcg catgaaagat
     1801 gagttcatgg caaatatgag ccatgaactt cgaaccccgc tcaatgcaat tttaggactt
     1861 tcagaaagcc tgctcgaaga aactgtcggc tccatcaatt cccgccaaca taaatatctc
     1921 aacaccatta gcgaaagcgg acgtcatcta ctggaattga tcaatgacat tctgtatctg
     1981 gcaaagatcg aatcagggca aatccactta gcctccaact ctgtaaacat caagaccttg
     2041 tgcgaagcct ctctccgcat cgttgaccaa ctggcaaaaa gcaaaaaaca agtcatgagt
     2101 ctcgaagtcg ccgatgagat taagtttgtc caagcagatg aacgtcgcct aaaacaaatg
     2161 gttgtcaact tgcttagcaa tgcgattaaa tttacgcccg agaacggtca agtagggata
     2221 cgggcattga tcgatgcgtc acgcaccttt ttaaacattg aagtctggga tacaggcatc
     2281 ggcatcaaag ctgatgacct ctcacacatc ttccaaccct tcattcaatt aaattctggt
     2341 ttggcgcgtg agtcatcggg cacagggctg gggttggcat tggtggcaca aatggctcgc
     2401 ctccacgggg gcagtattac agttcgcagc gaaattggaa aaggcagcag tttcattcta
     2461 agcttgccct ggacgccaga actgccagat gcccctcagg agcaataatt ggcgttttcc
     2521 gttatactcg cgccaatgcc aagcgaatgg atcgaaacca atcaaataaa acttcatgtc
     2581 gtga
//
LOCUS       DAOQJE010000539         2582 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_28731_length_2582_cov_2.519193, whole genome shotgun sequence.
ACCESSION   DAOQJE010000539 DAOQJE010000000
VERSION     DAOQJE010000539.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2582)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2582)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2582
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28731_length_2582_cov_2.519193"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            218..298
                     /locus_tag="PLT08_13080"
     CDS             218..298
                     /locus_tag="PLT08_13080"
                     /inference="COORDINATES: protein motif:HMM:NF013260.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE05451.1"
                     /translation="MNPYPCGYAGDAVKPCICAPAVFTKY"
     gene            complement(461..1414)
                     /locus_tag="PLT08_13085"
     CDS             complement(461..1414)
                     /locus_tag="PLT08_13085"
                     /inference="COORDINATES: protein motif:HMM:NF016114.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-binding transcriptional regulator"
                     /protein_id="HNE05452.1"
                     /translation="MPRIDELRLIARVARMYYEWEKSQSEIAKQLGLSQATVSRLLSR
                     SKEEGIIRISINLPGGVYTELEETLVKKFGLRDVIVVDSLEDNEKLIQRDLGAATAYF
                     LESAIRPNEVIGISSWSATLLAMVDALHPAPRKPGVKVVQILGGIGNPSAEAHATRLT
                     SRISQLVSGEAVYLPVSGVLATEAARDILAADEVAQQAIRLFDQVTTALVGIGSIEPS
                     PLLAQSGNIFAPQEMKLLRKANAVGDILLRFIDQNGNLVDTGLEKRVISMSLEQLRKV
                     SRAIGVAGGSRKYAAILGALRGHWINILVTDHFTAKRLANE"
     gene            1545..2420
                     /locus_tag="PLT08_13090"
     CDS             1545..2420
                     /locus_tag="PLT08_13090"
                     /inference="COORDINATES: protein motif:HMM:NF012670.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HNE05453.1"
                     /translation="MKTTSTPLDKQKILGLRRKSILYRKTLLEMIYKANAGHTGGSLS
                     CVDILNVLYNHVMNITPQNFNLIDRDHYIHSKGHSVEALYAVLADKGFFSYNLLDTVE
                     KYKSDFIGHPTRSVFGIEQNTGALGHGLSVAVGMALAAKRDGRSDRVFTIMGDGELTE
                     GSIWEASTSAAHYRLDNLIAIVDRNTLQISGPTEKVMRMEPLEGKFSAFGFAVRHVNG
                     NNIEELTALFENLPFETGKPNLVLAHTNKGNGISFMENQIQWHHRVPTEEEYAAALSE
                     LEEAEKKLEEPNGQL"
BASE COUNT          752 a          576 c          539 g          715 t
ORIGIN      
        1 acgacttgca atcccttttc ttctaccaga cggcgacaat ctgtttcctg ctcaattgga
       61 ctggaatttt caccttgtgt ttgtgacgat gtacggatgt aaattactgc tcggcgagac
      121 atttacatgt cctcaataat gaaaatactt aaacctgccg gttcaaatat accaatgaaa
      181 gggtaggatc gctaacaaat cagtcaggat cgctgccatg aatccctacc catgtgggta
      241 cgcaggagac gctgtcaaac cctgcatctg tgctccagcc gttttcacca aatattaaaa
      301 acgaatctct ggtccttaac agcaattttg ccaactccaa cccgaaggtc agagactgat
      361 gcaagcggcg atgagtcaac ttaatttggc tggaagtgaa gagatccggc agaggcgcgg
      421 tagtatcgtc caaagttgat gataaggtag gataagaaga ttattcgttt gctaagcgtt
      481 ttgcggtgaa atgatctgta acgaggatat ttatccaatg tccgcgcaat gcacccaata
      541 tggcagcata ttttcgtgaa ccgccagcta ccccgatggc acggcttact ttgcgtaact
      601 gttccaagct catggaaatg acgcgctttt ccaaccctgt gtccaccaaa ttcccatttt
      661 gatcaataaa tcgcaataaa atatctccta ctgcatttgc ttttcgtagc agtttcatct
      721 cttgaggggc aaaaatatta ccgctttgcg cgagtaaagg agaaggctca atggaaccga
      781 tcccgaccaa tgcggttgta acctggtcaa ataatcggat tgcctgctgt gctacttcgt
      841 ctgccgctaa aatgtcacgc gcagcttccg tggcaagtac accagatacc ggtaaatata
      901 cagcttctcc actaaccagt tgtgagatgc gcgatgttag ccgagtcgcg tgggcttctg
      961 cagatgggtt accaattccg cctaagatct gaacaacttt tacaccaggt tttcgtggtg
     1021 caggatgaag ggcatcaacc atcgcgagca aagtagcact ccaggaagaa atcccaataa
     1081 cttcgttagg gcgaattgct gattctaaaa agtaagcagt agcagcgcca aggtcacgct
     1141 gaatcaactt ttcattatct tccagactat ccacaacaat gacatcccgc aaaccgaatt
     1201 ttttgacaag agtttcctcc aactcggtat agacaccacc ggggagattg atcgaaattc
     1261 gaataatccc ctcttctttt gagcggctta ataatcttga gactgtcgct tgagataagc
     1321 caagctgttt tgcgatttca gactggcttt tttcccattc ataatacatg cgtgccacac
     1381 gcgcaattaa gcgtagttca tcaattcttg gcataggttt tctttctcca tgaaagattg
     1441 tactatattg aatagttatt cataatctat tgatttattc atcagcactc agtattatac
     1501 tgctaaatga attatattac aaaagtgaat aattattcaa ttttatgaaa accacttcta
     1561 ccccactcga taaacaaaaa atcctaggat taaggcgcaa atctattttg taccgcaaga
     1621 ccctattgga aatgatctat aaggcaaatg ccggacacac tggtggtagc ctatcctgtg
     1681 ttgacattct taacgttctt tataatcacg ttatgaacat aacccctcag aatttcaacc
     1741 tcattgatcg cgatcactat attcatagca agggtcattc agttgaagcc ttatatgctg
     1801 tattagcaga taagggtttc ttttcctaca atctcctaga tactgtcgaa aaatacaagt
     1861 ctgattttat tggtcatcct actcgaagtg tcttcggtat cgaacaaaat acaggcgcgc
     1921 ttggtcatgg actttccgta gcagtaggta tggctttagc agctaaacga gatggacgct
     1981 ccgaccgggt ttttaccatt atgggcgatg gtgaactgac agaaggttcc atttgggaag
     2041 cttcaactag cgcggctcac tacagactgg ataacctgat tgccattgta gaccgaaaca
     2101 cccttcaaat cagtggacct acagaaaaag tcatgcgcat ggagcccctt gaaggaaagt
     2161 tttccgcatt tgggtttgcg gttcgtcatg taaatggcaa caacatagaa gaattaactg
     2221 ccttatttga aaaccttccg tttgaaacag ggaaaccaaa tctagtcttg gcacatacta
     2281 ataaaggaaa tgggatcagt ttcatggaaa accaaattca gtggcatcat cgggtaccta
     2341 ctgaagaaga atatgctgct gccttatcgg aattggaaga agcagagaaa aaattggagg
     2401 aacccaatgg gcaactctaa tctaacaatt ggaaaaacta atctcgatac atttgccagc
     2461 actttaatag aacttgctac tcttgaccgt gaagttttgg ttgttacaag cgatacgcgc
     2521 gggtcagggc gtgttacacc atttgccgat aaatttcctg aacagatcgt ggagatcggt
     2581 at
//
LOCUS       DAOQJE010000540         2579 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_28780_length_2579_cov_7.341125, whole genome shotgun sequence.
ACCESSION   DAOQJE010000540 DAOQJE010000000
VERSION     DAOQJE010000540.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2579)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2579)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2579
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28780_length_2579_cov_7.341125"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2032
                     /locus_tag="PLT08_13095"
     CDS             <1..2032
                     /locus_tag="PLT08_13095"
                     /inference="COORDINATES: protein motif:HMM:NF024583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNE05454.1"
                     /translation="SELMLEEDGYLVVKAFTANQPNLKGDRVGREQAKLSWQAFDTKE
                     PVVLEDYATWEFRRDVYDVNPIHATADFPVMAGETCIGVLALGRDQAGYAFTNEQIQT
                     GILFARLVALVLDNVNLYDSALREIAERERAQISLQRSNEQQQIIVSLLRVGLENMTL
                     DQILGNILDGILSVGWMKLVPKAGIFLVDKETQKLRLHTHRNFSPPLLNLCAQVDMGR
                     CMCGRAAMQKQILFSDCLDERHEIRYEGIEEHGHYNVPILQGKEVLGVIVLYLPHGYQ
                     RSTDDETFLLAIADAISGVIGRKRVEALLQGSEILFRQIVENASDIIYRTDMQGMFTY
                     ANPAALNMMGFTSEQEVLGKNYLELTTPEARNRLKRVYDHQYLSRTNNTYYEFPAISM
                     KGEIIWVGQNVQLIWDGDKIVGFQALARNITQLRQAQEALLLARDQALEASRFKSQLV
                     SRVSHELRTPLGGILGFAELLKEGSFGSLNGSQMTAISNIVESTNFLTDTVNDLLDQA
                     QIEARSINLHNAYFKPADLMKKIAMTMSVLAGKKGLAFHTEVATDLPVELYGDEKRLQ
                     QIIVNLTGNAIKFTSHGEVNIRFVMASPDRWALKVQDTGVGIPEDERNSIFEPFQQLS
                     NSITRENRGSGLGLAIVKQLAELMSGEISLQSEAGRGSTFTVTFPLLQPVEN"
     gene            2093..2449
                     /locus_tag="PLT08_13100"
     CDS             2093..2449
                     /locus_tag="PLT08_13100"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNE05455.1"
                     /translation="MAKPLALVVEDDPQQNQIFTLALKNSFEVEAISDGSQAISRLTK
                     VTPRLIVLDINLPGASGAKVLEVIRKDKRFSSTRVILATANSQEAEVLREKSDIVLLK
                     PISPIQLREFAERLKS"
BASE COUNT          683 a          534 c          645 g          717 t
ORIGIN      
        1 tagcgagctc atgctggaag aggatgggta tttggtggtg aaagctttta ccgctaacca
       61 accaaactta aaaggtgacc gggttgggcg tgagcaggca aaactttcgt ggcaggcttt
      121 tgacacaaag gaacccgttg tacttgaaga ttatgccacc tgggaatttc ggcgcgacgt
      181 atatgatgta aacccgatac atgctacagc agatttcccc gttatggctg gtgagacatg
      241 tatcggcgtt ctcgccttgg ggcgtgacca agctggatat gcatttacga atgaacaaat
      301 tcaaacaggc attcttttcg cccgccttgt agcccttgtg ttagataacg ttaatttgta
      361 tgattccgcc ttgcgagaaa ttgctgagcg tgaacgtgct caaatttccc tgcaacggtc
      421 caatgagcaa cagcagatta tcgtatctct tttacgcgtt gggcttgaaa acatgaccct
      481 tgaccaaata ctgggaaata ttctggatgg aattctttct gtagggtgga tgaaacttgt
      541 tccgaaagcg ggcatctttc tcgttgacaa agagactcaa aaattgcgcc tgcacacaca
      601 tcgcaacttt tcccctcctt tgctgaattt atgtgctcag gtggatatgg ggcgctgcat
      661 gtgtgggcgt gctgccatgc agaaacagat cctgttttca gattgtttgg atgaaaggca
      721 cgagattcgt tatgaaggca tagaggagca cgggcattat aacgttccga ttttgcaggg
      781 caaggaagta ttgggcgtaa ttgtcttgta cctgccgcat ggatatcaac gcagtaccga
      841 tgatgagaca tttttgcttg caattgcaga tgctatctct ggcgtgattg ggcgtaagcg
      901 tgttgaagct ttgttgcaag ggagcgaaat cctgttccgt cagattgtag aaaatgccag
      961 tgatattatc taccgaacag atatgcaagg gatgtttaca tatgccaacc cggcagccct
     1021 taacatgatg ggtttcacaa gtgagcagga agtgcttggt aaaaattatc ttgaattaac
     1081 caccccagaa gcacgtaacc gtctaaagcg tgtgtatgac catcagtacc tcagtagaac
     1141 gaataatacc tattatgaat ttcctgccat atctatgaag ggtgaaatta tctgggtggg
     1201 gcagaatgtg cagttaattt gggatggtga taagattgtg ggcttccagg cattggcgag
     1261 aaatatcact cagttgaggc aggctcaaga agccttgctt ctcgcgcgtg accaggcatt
     1321 ggaagccagc cgtttcaaga gccaattggt ctcacgtgtg agccatgaac tgcgaacccc
     1381 tcttggaggc attttaggtt ttgccgaact gcttaaagag ggctcatttg gctctctcaa
     1441 tggcagccaa atgacagcca tttcaaatat tgttgaaagc accaacttct taacagatac
     1501 agtcaatgac ttgttagacc aggctcagat cgaagccaga tcgattaatt tacacaacgc
     1561 ttatttcaaa ccggctgatt taatgaagaa gatcgcaatg accatgtctg tattggctgg
     1621 taagaagggc ttggcatttc atacagaggt tgctaccgat ttgcctgtag agttgtatgg
     1681 agacgaaaag cgtttacagc agattattgt caaccttaca gggaatgcga ttaagtttac
     1741 ttctcatgga gaagtgaata ttcgttttgt tatggcatcc ccagaccgct gggctttgaa
     1801 agtgcaggat actggtgtgg gcattccaga agacgagcgt aatagtattt tcgaaccctt
     1861 tcaacagtta agcaattcca tcacacgtga gaaccgcggg tctgggttgg ggcttgccat
     1921 tgttaaacaa ttggcagagt tgatgagcgg agagatctct ttacaaagtg aagctgggcg
     1981 cggaagtacc tttactgtaa ctttcccgct gctacagcct gttgaaaact gagatacacc
     2041 actatcgggt atagtgaatc atttttgaaa attctaattt tttaggagat atatggcaaa
     2101 gccacttgca ctggtcgttg aagatgaccc acaacagaac cagattttta ctcttgctct
     2161 gaaaaactca tttgaagttg aagccattag tgatggttct caagctattt ctcgtcttac
     2221 caaagtgacg ccgaggttaa tcgttttgga tatcaatttg cccggggctt ctggcgcaaa
     2281 agtacttgag gtgattcgaa aagataagcg tttcagctct acgcgcgtca ttcttgccac
     2341 tgccaattcg caggaggctg aagttttgcg tgaaaaatcg gatattgtgc tactgaaacc
     2401 gatcagcccg attcagttac gggagtttgc agaacggttg aaatcttaaa ctcacaagtc
     2461 gaagcgcttg ccagcttgat tggcaagcgc ttttttatag actaagtttg cggcgacgag
     2521 atcttccatg gcatgcccca tggatttgta gaccgtcgct tcatgcgcgg accttctgc
//
LOCUS       DAOQJE010000541         2578 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_28806_length_2578_cov_6.518827, whole genome shotgun sequence.
ACCESSION   DAOQJE010000541 DAOQJE010000000
VERSION     DAOQJE010000541.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2578)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2578)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2578
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28806_length_2578_cov_6.518827"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..357)
                     /locus_tag="PLT08_13105"
     CDS             complement(<1..357)
                     /locus_tag="PLT08_13105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE05456.1"
                     /translation="MSKRIFISADHGMAIIYFLQSDVVSTLLEAGVEVVVLTDDETKD
                     KIASRFTHHGITFEGLRLKQASDYAKKVSPRMQWLLGYLRRVGGSRRINTEAMDSHVW
                     EVWGENGWKFRLGVWIP"
     gene            791..1141
                     /locus_tag="PLT08_13110"
     CDS             791..1141
                     /locus_tag="PLT08_13110"
                     /inference="COORDINATES: protein motif:HMM:NF015735.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HNE05457.1"
                     /translation="MKYMMLNFRNEAASANMTEEERMQEIQDYNAFFEYASKRATIIS
                     GEAVHPTSAATTIRVRNGKTLTTDGPFAETHEGLAGFYVIDCKDLDEAIEIASKIPVA
                     REGSVEIRPVIVFE"
     gene            1194..2438
                     /locus_tag="PLT08_13115"
     CDS             1194..2438
                     /locus_tag="PLT08_13115"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HNE05458.1"
                     /translation="MTSRQIAAQIETTFRQEHGKVLAALIAMLDDFALAEDALQDALI
                     AALEQWKQDGVPRNPAAWITTIAKRKAIDRIRRDSNFTRKQQVILDSLKGDEDDEEEE
                     METIPDERLKLMFTCCHPSLNLDAQVALTLSTLGGLSTHEIARAFLVPPTTMAQRLAR
                     ARTKIRDAGIPYRVYPAELLPERLDALLTVIYLIFNEGYVATSGDTLIRHELCSEAIR
                     LCRILVELLPNKPISAEARGLMALMLLHDSRRNARVNGNGELVVLEDQDRAKWDHDRI
                     NEGTAILDYALRLRNPGPYQVQAAISALHARAETAQKTDWAQIVKLYQTLFQMTASPV
                     VEVNQAVAMAMAGNPQAGLQILRRLKPEMEEYFPFHVAHADLLRRTNQREAAVDAYER
                     ALELCQNNLEHAYLRRKLDELA"
BASE COUNT          685 a          670 c          655 g          568 t
ORIGIN      
        1 gggaatccaa acacccaagc ggaacttcca gccgttctcg ccccacactt cccagacgtg
       61 actgtccatg gcttcggtgt tgatgcggcg cgacccgccc acgcggcgca agtagccaag
      121 cagccattgc atacgcggac tgaccttttt ggcgtagtcg ctggcttgtt tgagacgcag
      181 cccttcaaaa gttattccgt gatgtgtgaa acgtgaagcg atcttgtcct tcgtttcgtc
      241 gtctgtgaga acgacgactt cgacgcctgc ttcaagcaac gtcgaaacga cgtcactttg
      301 cagaaagtaa atgattgcca tgccgtggtc ggcggagatg aagatgcgtt tggacatcgc
      361 gggcaattct atcagggaat ggaagtccaa agtaaattca catctggcga gtcaaatggc
      421 aataccgtta ccctcgctga ccgttcaagc tggcattcca catttccatg gcagagttga
      481 ccaactcttt gtctgcgtct tgatttggtt acactattgt aaatttccgg tagcatcagt
      541 tgaaatataa aaaatgagag aaagattaaa gtgagaacaa agatgtcatt ttgatgaaat
      601 cccattcgac gtattgatag aaatgcaaaa aatcgcgttt cagcctttca ttcacataaa
      661 ccagttagga gtaactttcc atgaaataaa agatgtcatt ttgatagact ctcattcgac
      721 gtatcaatag aaatgcgaaa aagcgcgttt cagccttatc attcatataa accaattagg
      781 agtaattccc atgaaataca tgatgctcaa ttttcgtaac gaagccgcca gcgccaacat
      841 gacggaggaa gaacggatgc aggagataca ggattacaac gccttttttg aatatgctag
      901 caagcgcgcc acaatcattt caggcgaagc cgtccacccc acctctgctg ccaccaccat
      961 ccgcgtccgc aatggcaaaa cgctaaccac cgatggtccg tttgccgaaa cccacgaagg
     1021 tcttgccggt ttctatgtca tcgactgcaa agaccttgat gaagccatcg aaatcgcctc
     1081 gaaaatcccc gttgcgcgcg aaggcagcgt cgaaatccgc ccagtgatcg tatttgaata
     1141 aacgtgcatg taggggcgga tttgaaatcc gcccacctga tgaaggatag aggatgacct
     1201 caagacagat tgcggcacaa atcgagacca ccttccggca ggagcatggc aaagtccttg
     1261 cagcgctgat cgccatgctg gatgatttcg cgctggcaga agatgctttg caggatgcgt
     1321 tgattgcggc attagaacag tggaagcagg atggtgtacc ccgtaatccc gctgcatgga
     1381 tcaccacgat tgcgaaacgt aaagcgattg accgcatccg ccgcgactcg aacttcaccc
     1441 gcaaacagca ggtcatcctc gactccctta aaggggatga agacgatgag gaagaagaaa
     1501 tggaaactat cccggatgaa cgcctgaagc tgatgttcac ctgctgccat ccctcgctca
     1561 atctggatgc acaggtcgca ctcaccctga gtacgttagg cggcttatcc acgcacgaaa
     1621 tcgcccgcgc ttttcttgtc ccgccaacaa cgatggcaca gcgtctggca cgagcacgca
     1681 ccaaaattcg ggacgcaggc atcccctacc gtgtttatcc cgccgaactg ctcccagagc
     1741 ggttggacgc actgctaacc gtgatctatc tgatctttaa tgagggttat gtcgccacca
     1801 gcggcgatac cttgattcga cacgaacttt gcagcgaagc gattcgctta tgccgtattc
     1861 tggtggaact gctgcccaat aagccgatca gtgcggaggc acgagggcta atggcactga
     1921 tgctcttgca tgactcccgg cgaaatgccc gtgtgaacgg caacggtgaa ctggttgtgc
     1981 tggaagatca ggatcgcgca aagtgggatc acgaccgcat aaacgaaggg acagcgatcc
     2041 ttgattacgc cctgcggctg cgaaatcctg gtccttatca ggtacaggca gcgattagtg
     2101 ctctacacgc acgggcagaa accgcccaaa agactgactg ggcgcaaatc gtaaaactct
     2161 accagacgct gttccagatg accgcttcgc cagtcgtgga agtcaaccag gcagtggcga
     2221 tggcgatggc gggaaaccca caggcagggc tgcaaattct gcgccgcctg aagccggaaa
     2281 tggaagaata ttttccgttt catgtcgccc acgccgatct cttacgccgc acaaatcagc
     2341 gtgaagccgc cgtagatgcc tatgaacggg cgctggaatt gtgccagaat aatctggaac
     2401 acgcctatct gcggcgcaag ctggatgaac tggcataaaa tcgctaagaa gatgagttcg
     2461 tgtgcgaacc acgcggtaca gtcaaggggc aaaggggaga tggatatttg gttggttgtt
     2521 tcggctaagt gaattcgtta cgagtaatac caactgaaaa tcttgttata caccgcca
//
LOCUS       DAOQJE010000542         2578 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_28811_length_2578_cov_3.023781, whole genome shotgun sequence.
ACCESSION   DAOQJE010000542 DAOQJE010000000
VERSION     DAOQJE010000542.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2578)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2578)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2578
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28811_length_2578_cov_3.023781"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1513)
                     /locus_tag="PLT08_13120"
     CDS             complement(<1..1513)
                     /locus_tag="PLT08_13120"
                     /inference="COORDINATES: protein motif:HMM:NF024883.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HNE05459.1"
                     /translation="MANNTIPTAFDIKAWREIFILSILRFASVTGIVVIIGSFSTATP
                     LDRFLFIGFYLVVVAITVLPVPYALRAYVFLLILFAVGVNSIIAWGPWLDGSIFLLTS
                     IAMASLILDNRIDVIGLILSIIFLIALAVLNQLGSFEPIAEQSPVTKGVDWIGYIANY
                     VASGIITVIAINQFKGAFTRVSDQMQIAYEALKVEQVKLEDKVRERTEELETRMTQLR
                     YSATTARVVAEAREIGELMDTAVSLMSERFGYYHIGLYILDEPGKSAYLQASSSETGK
                     SLIGQILRLEADRRNPITSAILNNRPIISSDSDTATTFVKDPNFPLTRSRMVLPLAVR
                     SLVIGFLDLHSDQTKAFSPEDAEILQTLADLTAISFDNVRLLDETRYLLKQLETNTSI
                     QTQMTWSKLTTRHKPAYQYTPAGVRPIFSHDKRSSEEGLKIPIQLHGQAIGFIKLRRK
                     GLVTTWSEREQVLVEKIAVQVGLALENSRLVDEAQKNALRNQMIANFSSSVRET"
     gene            complement(1503..>2578)
                     /locus_tag="PLT08_13125"
     CDS             complement(1503..>2578)
                     /locus_tag="PLT08_13125"
                     /inference="COORDINATES: protein motif:HMM:NF024883.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HNE05460.1"
                     /translation="MFGVVIIIGFRQLEIEFAAAQKQIDSSVEALREERNNLENRVQE
                     RTVQLRRINDVGRAIAAILAPNELLERAAKLIQNEFDFYYTSFYLLDSTGQWLELKEA
                     QGEAGKILKENKHRLSVESKSIIATAVRTKSVQILTDSAQIRTEHPLFPYTRSMIVVP
                     LVLGETIFGALEMHASKENSFLQQDIDAYQNMANGIAVSIENSRLFQEAQQSIMEMQA
                     TQRQYLQGAWSSLTSEKDLDYALGDGDLADNNPIEIPLALRNQIIGQIELANTAEWTN
                     EQKSLIEAIAVQATLALENARLVEESQATATQERITNEIISKIWTSANMDGILQTTVR
                     ELGRNLEASEVEIEILMDGQSGE"
BASE COUNT          678 a          546 c          606 g          748 t
ORIGIN      
        1 gtgtctctcg aacagaactt gagaaatttg caatcatttg attgcgaagc gcattctttt
       61 gcgcttcatc caccaggcgg ctgttttcca gagcaagccc tacctgtact gcgatctttt
      121 cgaccagaac ttgttctctt tcagaccatg ttgtgacaag acctttacgt ctaagtttaa
      181 tgaagccaat tgcctgacca tgcaattgga tggggatttt taatccttct tcggatgacc
      241 ttttgtcatg actgaaaatt gggcgtaccc ctgcaggagt atattgatag gcaggtttgt
      301 gacgagtggt cagttttgac caggtcattt gagtctggat ggaggtgttt gtctcaagtt
      361 gtttgagcag gtatcgggtt tcgtccagga ggcgtacatt gtcaaatgag atggcagtta
      421 ggtctgccag ggtttgcagg atttcggcat cctctgggct aaaggctttt gtctgatctg
      481 aatgaagatc taaaaagcca attacaaggc tgcgaacggc gagtggtaag accatgcgcg
      541 atcttgtcag ggggaaattg ggatccttga caaaggtggt tgctgtatcc gagtcggaac
      601 taatgattgg acggttgttc aaaatagcag atgtaattgg gtttcttcga tcggcttcaa
      661 ggcgaaggat ttgcccaatt aaggatttac ctgtttcaga tgacgatgct tgaaggtatg
      721 ccgactttcc aggttcatcc aggatatata acccaatgtg ataataccca aaccgttcag
      781 acatcaagct tacggcagta tccatgagtt cgccaatttc acgggcttcc gccacgacac
      841 gggcagtggt agcagaatac cgtagttgag tcatacgggt ttcaagttcc tctgtgcgtt
      901 ctcggacttt atcttcaagt ttaacctgtt ccacctttag agcttcgtaa gcgatctgca
      961 tttgatcgct tacacgggta aatgcgcctt tgaactgatt gatggcgatg accgtgataa
     1021 tgcctgaagc aacataattg gctatgtatc ctatccaatc cacccctttg gtcacggggg
     1081 actgttctgc aattggttca aatgaaccta gctgatttag gactgccaga gcaattagga
     1141 atatgatact taagattaaa ccaataacgt caatgcgatt atccaggata agagaagcca
     1201 ttgcaatgct ggttaaaaga aaaatactac catccaacca gggaccccat gcaatgattg
     1261 aattcacgcc aactgcaaaa aggataagaa gaaagacata tgcccgtaaa gcgtatggaa
     1321 cgggtaaaac cgttattgca acaacaacca gataaaaacc aatgaataaa aaacgatcga
     1381 ggggggtcgc tgtggaaaac gaccctatga ttacaacaat gccagtgacg ctggcgaaac
     1441 gcaaaataga caaaatgaat atttctcgcc aggctttgat gtcaaaagcg gtggggatgg
     1501 tgttattcgc cactttggcc atccattaat atttcaattt ctacttcgga agcctcgagg
     1561 ttgcgtccaa gttcacgaac tgtcgtttga agaatgccat ccatatttgc ggatgtccat
     1621 attttggaaa tgatttcgtt tgtgattctt tcctgagtgg ctgttgcctg actttcctct
     1681 accaaacggg cgttttccaa tgccaatgta gcttgaacag cgattgcctc gatcaggctt
     1741 ttttgttcat ttgtccattc tgctgtattt gccaactcga tttgaccaat gatctgattt
     1801 ctcagggcaa gagggatctc aatgggattg ttatctgcca aatcgccatc acccaatgca
     1861 taatcgagat ccttttcaga ggttaaagaa ctccaggcac cctgtaagta ttggcgttga
     1921 gtagcttgca tttccatgat gctttgttgg gcttcctgga aaagacggga attttcgatc
     1981 gaaaccgcaa taccgtttgc catgttttgg taggcatcaa tatcttgttg aaggaatgaa
     2041 ttttccttgg aagcatgcat ttccaatgca ccaaatatgg tttcgcccaa cacaagcgga
     2101 acaacgatca tcgaacgtgt ataaggaaaa aggggatgtt ctgtgcgaat ttgtgcacta
     2161 tctgtcagga tttgcactga tttggtgcgc acagctgtgg cgataatgct tttactctct
     2221 acactgaggc gatgcttatt ctcttttagg attttcccgg cttcgccttg tgcttcttta
     2281 agctccagcc attgcccggt tgaatcaagc agataaaagg atgtatagta aaaatcaaat
     2341 tcattttgta tgagtttggc cgcccgttca agaagttcat ttggggctag gatcgcggca
     2401 attgcacgcc caacatcatt gatgcggcga agttgtactg tacgttcctg tacgcgattt
     2461 tcgagattat tcctttcttc gcgaagggct tctacagagg agtcgatttg tttctgcgct
     2521 gctgcaaatt cgatctccaa ctggcgaaag ccaatgataa taaccactcc gaacatga
//
LOCUS       DAOQJE010000543         2574 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_28871_length_2574_cov_6.631203, whole genome shotgun sequence.
ACCESSION   DAOQJE010000543 DAOQJE010000000
VERSION     DAOQJE010000543.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2574)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2574)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2574
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28871_length_2574_cov_6.631203"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1131)
                     /locus_tag="PLT08_13130"
     CDS             complement(<1..1131)
                     /locus_tag="PLT08_13130"
                     /inference="COORDINATES: protein motif:HMM:NF014750.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016625 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, iron-sulfur
                     protein as acceptor [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde ferredoxin oxidoreductase N-terminal
                     domain-containing protein"
                     /protein_id="HNE05461.1"
                     /translation="MTDYKSQIWRINTRTQELKREPVPETWQHLGGRGLIARIMVDEV
                     DATCDPLGPGNKLIFTPGLLVGHILSSTDRISVGGKSPLTGGIKEANAGGRTGYHMAF
                     MGIHALILEDMPEKDGYWVLHLSLNDGAKWERADELVGLGVYETAPKLIEKYGDKVAI
                     SLIGPGGEMRMKSAGIQNLDKDKIPSRIAARGGLGAVMGSKGLKAIVFDHIGGQKPPI
                     ADVEAFKVAQKDYTQSVMNHPQSVTYREYGTQAMPQMTQNFAALPTHNFSRGTFDDVE
                     KISGETMREFILNRGKPSDPSHACMAGCTIKCSNVFGGEDGKIIVSPLEYETVGLMGT
                     NLDINSLDSIGRMNWQVNDLGLDTIEVGGALGVAAEAGIMRWG"
     gene            complement(1134..2495)
                     /locus_tag="PLT08_13135"
     CDS             complement(1134..2495)
                     /locus_tag="PLT08_13135"
                     /inference="COORDINATES: protein motif:HMM:NF005453.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HNE05462.1"
                     /translation="MNTTLDRSKLEKLLKQEEELFHKTHPKSYELYQRARKSLHGGVP
                     MLWMIRWAGSFPVFVKEAKGAHFTDVDGNNYIDFCLGDTGAMTGHAPEATVKAITEQI
                     QKGITLMLPYEDVIWVGEELQRRFKLPYWQFALTATDANRFALRMARLITGRPKVLVF
                     NYCYHGSVDETFITLDEEGTPISRPNNMGPQIDPRETSKVIEFNDIAALETALSARDV
                     AVVLAEPAMTNIGIIHPDPGYHEALRALTRKYGTYLIIDETHTICAGPGGYTASYGLE
                     PDFITLGKPLAGGVPAAIYGFTEEVSKAFSAKLAMDDADVGGIGGTLAGNALSIAAMK
                     ATLQNVLTDEFYAKATTLQEQFTAGVEGVIAEFKLPWIVKRLGNRSEYWFRPTPPKNG
                     GEAHAAVDPELDRYMHLYALNRGILMTPFHNMALISPETTQDDVDHHTKVFREAVKSL
                     FGE"
BASE COUNT          646 a          668 c          613 g          647 t
ORIGIN      
        1 gccccagcgc ataattcccg cttcggcagc gacccctaat gctccaccta cttcgatggt
       61 atccaaacca aggtcattaa cttgccaatt catgcgtcca atggagtcga gcgaattgat
      121 atcaaggttg gtgcccatca aaccaaccgt ctcatattcc aacggcgaaa caatgatctt
      181 gccatcttca ccgccaaaca cgtttgagca cttgatcgta caccccgcca tacaagcatg
      241 agaggggtcg gagggtttgc cacgattcaa aataaactcg cgcatggttt cgccgctaat
      301 cttttctaca tcgtcaaaag tgccacgcga gaaattatga gttggcaacg ctgcaaaatt
      361 ctgggtcatc tgtggcatgg cttgcgtacc gtactcgcgg tatgtcacag actgcggatg
      421 gttcatgacc gattgggtgt agtctttctg agcaaccttg aatgcttcta catcagcaat
      481 tggaggtttt tgtccgccta tgtggtcaaa cacaatagct ttcaaaccct tcgagcccat
      541 caccgcacct aacccgccac gcgcagcaat acgcgaaggg attttatctt tgtctagatt
      601 ctgtatccct gctgacttca tacgcatctc gccaccagga ccgatgagtg aaatggcaac
      661 tttatcgcca tatttttcga tcagtttcgg agcagtttca tacacaccca agccaacaag
      721 ctcatcggct cgctcccatt ttgcgccatc gttcaaagac aagtgcaaaa cccagtagcc
      781 atctttttcc ggcatatctt caagaatgag tgcatggata cccataaacg ccatgtgata
      841 gcccgtgcgc ccgcccgcat tggcttcctt gattccgccc gtcagcggag acttaccgcc
      901 caccgaaata cggtctgtgg aggaaaggat atgccctacc aacaagccgg gtgtaaaaat
      961 caacttattg ccgggtccaa gtgggtcaca tgttgcatcc acttcatcca ccatgatgcg
     1021 tgcaatgagt ccgcgcccgc cgaggtgttg ccaggtttca gggacaggtt cacgcttcaa
     1081 ttcttgagta cgagtattaa tacgccagat ttgagattta tagtcagtca ttatcactct
     1141 ccgaacaaac tcttcacagc ttctctaaaa acttttgtat gatgatccac atcatcttgg
     1201 gtagtttcag gtgagatgag cgccatgtta tgaaagggcg tcatcaatat accgcgattt
     1261 agcgcataca agtgcatgta acgatccaat tctggatcta cggcagcatg agcttcgccg
     1321 ccgttctttg gaggtgtggg acggaaccag tattcagagc gatttcctaa acgcttcaca
     1381 atccacggaa gcttgaattc tgcaatcacg ccttcgactc ccgctgtaaa ctgttcctgt
     1441 aacgtcgtcg ctttggcata gaactcatca gtgagcacat tctgaagtgt tgccttcatc
     1501 gccgcaatgg acagagcatt acctgccaat gtgccaccaa taccaccgac atcggcatcg
     1561 tccatggcaa gtttagcaga aaatgctttc gagacttcct ctgtaaaacc atagatcgcg
     1621 gcgggaacgc ctccagcgag aggtttgccg agggtgatga aatcaggctc aagaccgtaa
     1681 gaggcggtgt atccgccggg accagcacaa atggtgtgcg tctcgtcaat gatgagatag
     1741 gtcccgtatt tgcgcgtcaa tgcacgcaaa gcttcatgat agccggggtc agggtggatg
     1801 atgccaatat tcgtcatggc aggttcggca agcacaactg ccacatcgcg agcggagagc
     1861 gccgtttcga gggcggcaat atcattgaat tcaatgacct tgctggtttc gcgcgggtcg
     1921 atctgcggac ccatattgtt ggggcgagag atgggcgttc cttcttcatc aagcgtgatg
     1981 aaggtttcat cgacagaacc gtgatagcaa tagttgaaaa ctaaaacctt ggggcgccct
     2041 gtgataaggc gtgccatgcg aagcgcaaag cgattcgcat ctgtagcagt aagagcaaat
     2101 tgccaatacg gcaacttgaa gcggcgttga agttcctcac ctacccaaat gacatcttca
     2161 tagggcaaca ttagggtaat gcccttttga atttgctcag taatggcttt aaccgtcgct
     2221 tcgggagcgt gacctgtcat ggcgcccgta tcgccgagac aaaagtcgat atagttgttg
     2281 ccatccacgt ctgtaaaatg tgcccctttg gcttctttga caaacaccgg aaaagacccc
     2341 gcccagcgaa tcatccacaa catgggcaca ccaccatgca gagatttgcg cgcccgttga
     2401 tacagctcat acgatttggg gtgggtctta tgaaagagtt cttcttcttg tttgagaagt
     2461 ttttcgagtt tggaacgatc aagggtagtg ttcatggttc tctctatttt ttcaggattg
     2521 tcaaaatctc aaatcgactg tatattaccg aagcgagggg gcggactatc aacc
//
LOCUS       DAOQJE010000544         2574 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_28874_length_2574_cov_5.714569, whole genome shotgun sequence.
ACCESSION   DAOQJE010000544 DAOQJE010000000
VERSION     DAOQJE010000544.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2574)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2574)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2574
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28874_length_2574_cov_5.714569"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..431
                     /locus_tag="PLT08_13140"
     CDS             <1..431
                     /locus_tag="PLT08_13140"
                     /inference="COORDINATES: protein motif:HMM:NF041518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="choice-of-anchor Q domain-containing protein"
                     /protein_id="HNE05463.1"
                     /translation="VFTPEENFITNTILAGNEDLSSRVKHRDCSGIIESGGYNLLQSP
                     QGCTFTGSLTGNILNQSPNLLPLDFYLGNDVTQTPPQGLTPTHAYPPGSLGAGVDDGN
                     PAGCIGTTAQNGVSRHLAATGGGGPGNCDIGAFELVGPDL"
     gene            complement(505..939)
                     /locus_tag="PLT08_13145"
     CDS             complement(505..939)
                     /locus_tag="PLT08_13145"
                     /inference="COORDINATES: protein motif:HMM:NF016535.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HNE05464.1"
                     /translation="MDTLIIIILIGLAGGMAIGLQGPLSSMITQKLGPLESVFIIHLG
                     GAIAALIPMLFFGSKLGQWKSVPWYALVAGAFGLIVITAMGYMIPRIGAAGALITLLA
                     GQILVASILDHFGLLGMTPRAIDLQRVIGFGVVMVGVWLTVK"
     gene            complement(988..2337)
                     /locus_tag="PLT08_13150"
     CDS             complement(988..2337)
                     /locus_tag="PLT08_13150"
                     /inference="COORDINATES: protein motif:HMM:NF012608.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malic enzyme-like NAD(P)-binding protein"
                     /protein_id="HNE05465.1"
                     /translation="MAWEPKIMRKVRIRNSQKKGVLGKLLTAVAEAGGNVGSINLLTE
                     TSRHVVRDITITAEDEKSLAAVLAAMETNEGTKILEVRDEVLELHQKGKIAIRSRYPI
                     ESLTTLRRVYTPGVAEVCNRIAKDPSQARLYTSISHMVAIVTDGTAVLGLGDIGPLAG
                     MPVMEGKAMLMETLVGLSGIPILLNTKDTDQIVNTVAAIAPTFAAIQLEDISAPRCFE
                     IEERLQAMLDIPVLHDDQHGTAVVSTAALMTATREAGMDMKKAVIGQIGLGAAGQAIG
                     QMMMRLTGNPVHGADLSPEALNRFEKLGGIKSNLKDIMANCDIVVATTGAPNLIKPEM
                     VRKGQIILALSNPNAEIDPEVAMERGAAFAADGKSVNNVLGFPGIFRGAVDSNAPRIT
                     QEMLLAAAETIASMTPSGELVPNPLDKKVHHAVARAVAETAMKQGIARADYVPYVEE"
BASE COUNT          649 a          737 c          557 g          631 t
ORIGIN      
        1 ttgtgtttac cccggaggaa aattttataa ccaatactat tcttgccgga aatgaagatc
       61 tatcatcaag agtcaagcat cgcgattgca gcggaataat agaatcgggt ggatataatc
      121 tattgcaatc gcctcagggc tgtacattta ccggctcgct aaccgggaat atccttaacc
      181 aatcgccgaa tctcttgccg cttgattttt acttgggcaa tgacgttact caaaccccgc
      241 cgcaaggttt aaccccaaca catgcatatc ctcccggcag tttaggagct ggtgtagatg
      301 atggaaaccc tgccggttgc attggtacga ctgcacaaaa cggtgtgtca cgtcatctcg
      361 ccgcaacagg tggaggtgga cccggcaact gtgatattgg cgcttttgaa ttagtcggac
      421 cagacttgta aataataaga tccctcaagt tttcttgagg gatcttatta tttttcttta
      481 ttgaacaatt tctaaaattt cccctcattt taccgtcaac cacaccccca ccatcactac
      541 accaaacccg atcacccgct gcaaatcaat cgctcttggt gtcatgccga gcagaccgaa
      601 atgatcgagg atggaagcca ccaaaatttg cccagccagc aaggtgatca acgcacctgc
      661 tgcgccaatg cggggaatca tatatcccat cgccgtgatg acgatcaacc caaaagcacc
      721 tgcaacaagc gcgtaccatg gcacgctttt ccactgtccc aatttagagc cgaagaagag
      781 catgggaatc aacgccgcaa tggctccgcc caagtgaatg ataaagacgc tttccaacgg
      841 tcctagcttt tgtgtgatca tgctcgagag tggaccttgc aaccctatcg ccatcccgcc
      901 cgcgagaccg atcaaaataa tgatgatgag agtatccatt taatccttat acttcgcaaa
      961 caacagtctg cggtctattg tccaagttta ttcttccaca tacggcacat agtctgcacg
     1021 agcaatgccc tgcttcattg ccgtctctgc cacagcacgc gccacagcat gatgaacctt
     1081 tttatccaac gggttgggca ccaattcacc agacggcgtc atactggcaa tggtctctgc
     1141 cgctgcaagc aacatctctt gtgtaatacg cggcgcattc gagtccactg ccccgcggaa
     1201 gatgccggga aagcccaaaa cattgttcac agattttcca tccgctgcaa atgccgcacc
     1261 ccgctccatc gccacttcag ggtcgatctc agcattcggg ttcgaaagcg cgaggatgat
     1321 ctgtccctta cgcaccatct ccggcttgat caggtttggc gcacctgtag ttgccacaac
     1381 aatatcgcag ttcgccataa tgtctttcaa attggattta atgccgccca acttttcaaa
     1441 acgattcaac gcctctggac tcaagtccgc gccatgaacg ggattgcccg tcaaacgcat
     1501 catcatctga ccgatcgcct gcccagccgc cccaagcccg atctgcccga tgacggcttt
     1561 cttcatatcc atgccggctt cgcgagttgc cgtcatcaat gccgcggtag aaaccaccgc
     1621 cgtcccatgt tggtcatcat gcaatacagg gatatccaac atggcttgca agcgttcttc
     1681 aatttcaaaa caacgcggag ccgaaatatc ctccaattga atcgccgcaa aggttggggc
     1741 aattgccgcc acggtattca caatctggtc tgtatctttt gtattcaaca aaatcggaat
     1801 gcccgaaagc ccaaccaacg tttccatgag catggctttg ccttccatta ccggcatccc
     1861 cgccagtgga ccgatatccc ccagccctag cacggctgtc ccatcggtaa caatcgccac
     1921 catgtggcta atgctcgtat acagccgagc ttgcgatggg tctttggcaa ttcgattgca
     1981 aacctctgca acgccagggg tatacacgcg gcgcagagtg gtcaatgatt caatcgggta
     2041 tcgcgagcgg atggcgatct tgcctttttg gtgcaactcc aacacctcat cgcgcacttc
     2101 gagaatcttt gtcccttcgt tggtttccat cgccgccaag accgcagcta gagatttttc
     2161 gtcctctgcg gtgatggtaa tgtcccgaac aacatgccgg gaggtttccg tcaacaggtt
     2221 gatacttccg acatttccac ccgcctcagc gacagccgtt aataatttac ccaacacccc
     2281 ttttttctgt gaatttcgaa tgcgcacctt gcgcatgatc ttcggttccc acgccatggt
     2341 tatctccttt ttggttatgc tcattttaac gcgaataccg cgaagtcgct tcgcggtatt
     2401 ggaacagaca actttataaa gaaattgagt ttacatcaat aacttgtaaa actcagcata
     2461 tgagcggatc acgtcacatt cggcatcagg gaagagtccg gttgggtcga agagcacagg
     2521 caaaagtcca gaacgaagcg caccaaccac atctgcaaaa tagttatcgc ctac
//
LOCUS       DAOQJE010000545         2574 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_28875_length_2574_cov_5.485113, whole genome shotgun sequence.
ACCESSION   DAOQJE010000545 DAOQJE010000000
VERSION     DAOQJE010000545.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2574)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2574)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2574
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28875_length_2574_cov_5.485113"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(128..1282)
                     /locus_tag="PLT08_13155"
     CDS             complement(128..1282)
                     /locus_tag="PLT08_13155"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HNE05466.1"
                     /translation="MPENPYLNVLLMAALTVVFIIACSYGLGHLAKSIARLRGASPKK
                     QESTFAGYFFAAPWIVGFLIFVIVPLGFSLYWSFTDYRIASGEPTLWVGAKNYQTILF
                     DDESFRSSIINTLYLTVIGLPLQIGVALFLAVLLNQKMRGERIFRMAFYLPVILGFNT
                     AVLLCWRLMLNTGTGIINQIIRGITEAFPPFGYISRGIIYIQEVSAAFFLGLNTGKYN
                     LMMKVIEAGFPAENRVPLWVQSPLWTKMSVVLLMVWGCGAMMLIFLAGLNNIPKELHE
                     AAEVDGASPWQRFWKISFPLLTPYIFYNLVVGMISALQIFEPIFVLYRDNQPVVSSTI
                     SMVYYLWQKSFSHFEIGYGSAISWVILVIIMIVTVIQFRLQDRWVTYDIY"
     gene            complement(1346..>2574)
                     /locus_tag="PLT08_13160"
     CDS             complement(1346..>2574)
                     /locus_tag="PLT08_13160"
                     /inference="COORDINATES: protein motif:HMM:NF024808.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HNE05467.1"
                     /translation="NAEVTLKVYLLDYTPDTIAWLKEEINPAFEAAHPGVKVEITEGS
                     WSGWDTTFSGFFAAGEGPDIINLGSEMNTLYGENLANMDEYLGEGAWDEISNFGPALE
                     NAKYEGSLRGLPIFTAPRYVFCRTDLMESAGWTTGTPKNFEEWKTFASQASVIDPATN
                     SLSQQALVPVDAGSMADWQWWLLVFYSLGGELYKADGTPNFDSPEALAATQFLLDMRQ
                     ATYGEAVNAVGSLPAGQGSVIDVDDATGKDNGAVCLAHSGWAAPAFDRPIWDNVSIEP
                     FYGDPENFPNSKPVVLAFNDWLAVPEYSQNKELAAEWLKMAFSKEGNNKWNETMGLIP
                     ARNDSQYGYVTESAQLQREAELASQYGVGFAGIKEAAKLSTIMQDALGKLITEELTVD
                     EVNAKIQEEYTAALGQ"
BASE COUNT          725 a          614 c          659 g          576 t
ORIGIN      
        1 ccactgtttt gaatgaggag gagatcatcc aaaacagcgg gaaactcatg aggactgttg
       61 ccccggtcaa cagtgtgtag agcacaatgc tttttagaat gggaacatat tttttcattg
      121 tgctctccta ataaatatcg taggtcaccc agcggtcttg caagcggaac tggataactg
      181 taacgatcat aataatgaca agaatgaccc atgaaatggc agagccatac ccgatttcga
      241 aatgactaaa actcttttgc caaaggtaat aaaccatgga aatggttgaa gaaaccaccg
      301 gttggttatc acgatagagg acgaagatcg gttcaaagat ctgtaaggca ctgatcatgc
      361 ctacaacaag gttataaaaa atatacgggg tgagaagtgg gaaggaaatc ttccagaaac
      421 gctgccaagg gctagctccg tctacttcgg cggcttcatg caattccttg ggaatattgt
      481 tcaatcccgc gagaaagatc aacatcatgg cgccgcagcc ccagaccatg agcaagacaa
      541 ccgacatttt tgtccacaac gggctttgca cccaaagtgg aacgcggttc tcagccggaa
      601 agcctgcttc gataaccttc atcatcaggt tgtatttgcc agtattcaag ccgaggaaga
      661 atgcggcaga aacttcttgt atatagataa tgccacggct gatatagcca aatggcggga
      721 aagcttcggt aattccgcga ataatttgat taataatgcc cgtgcctgtg ttcaacatca
      781 agcgccaaca caaaaggaca gccgtgttga agcccaaaat cacagggaga taaaacgcca
      841 tacggaagat acgctcaccg cgcatttttt ggttgagcaa cactgccaaa aacaatgcta
      901 cgccgatctg caaaggcaaa ccaatcacag tgagatataa ggtgttgata atggaagagc
      961 gaaaactttc gtcgtcaaaa agaatagttt gataattttt tgcccccacc cacaaggtag
     1021 gctcgcctga ggcgatacgg taatcggtaa aactccaata gagagaaaac ccgagtggaa
     1081 caatcacaaa aataaggaag ccaacaatcc atggtgcggc aaaaaaatag cccgcaaagg
     1141 tactttcttg ttttttcgga gatgcgccgc gtagcctggc aatagacttg gctagatgcc
     1201 ccaacccata actacatgca atgatgaaca cgactgtcaa tgctgccata agcagcacat
     1261 tcagatatgg attttcaggc atggcgagtc tcctctaaaa atattgctcg catggggcaa
     1321 gccccatgcg agcaataaag tttctttact gaccgagagc ggcagtatat tcttcctgga
     1381 tctttgcgtt cacttcgtca acagtaagtt cttcagtaat caacttgccg agagcatcct
     1441 gcatgatggt ggagagtttg gcagcttcct tgatgccggc aaaaccgaca ccatactggg
     1501 aagccaattc cgcttcacgc tggagctggg cagattcggt cacataacca tactgtgagt
     1561 cgttacgggc agggatcaag cccatggtct cattccactt gttgttgcct tctttgctga
     1621 aagccatctt gagccattcg gcagcgagtt ctttgttctg gctgtattca ggaacggcga
     1681 gccagtcatt gaatgcgagc acaacgggct tgctgttggg gaagttctca gggtcaccat
     1741 agaacggttc gatggaaaca ttgtcccaga tggggcgatc aaaagcagga gcagcccaac
     1801 cggagtgtgc gagacacacg gcgccgttat ccttaccagt ggcatcatca acgtcgatca
     1861 cagagccctg accggcgggg agagaaccga cagcgttcac agcttcgcca taggtcgctt
     1921 ggcgcatgtc gagcaagaac tgagtcgcag ccaacgcttc aggagagtcg aagttggggg
     1981 tgccatcggc tttgtagagt tcgccaccga gggagtagaa cacgagtaac caccactgcc
     2041 aatccgccat ggagccagcg tccacaggga cgagagcttg ctgagaaagg gagttggtag
     2101 cggggtcgat cacagaagcc tgagaagcaa aggtcttcca ttcttcaaag ttcttggggg
     2161 tgccagtggt ccagccagcg gattccatca agtcggtgcg gcagaacacg tagcgaggag
     2221 cagtgaagat cggcaaaccg cggagtgagc cttcgtactt ggcattctca agtgcaggac
     2281 caaaattgga aatttcatcc caagcgcctt cgccgagata ttcatccatg ttggcgaggt
     2341 tttcaccata cagggtgttc atttcggagc cgaggttgat gatatcggga ccttcgccag
     2401 cagcaaagaa gccgctgaag gtggtatccc aaccagacca ggaaccttcg gtgatctcaa
     2461 ccttcacgcc ggggtgagcg gcttcgaaag cagggttgat ctcttccttt aaccatgcaa
     2521 tggtgtcagg ggtataatca agcaggtaaa ccttcaaggt tacctctgcg tttg
//
LOCUS       DAOQJE010000546         2571 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_28927_length_2571_cov_4.963037, whole genome shotgun sequence.
ACCESSION   DAOQJE010000546 DAOQJE010000000
VERSION     DAOQJE010000546.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2571)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2571)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2571
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28927_length_2571_cov_4.963037"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            150..1319
                     /locus_tag="PLT08_13165"
     CDS             150..1319
                     /locus_tag="PLT08_13165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016293395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HNE05468.1"
                     /translation="MTDLPIYPLTNEHKMLRDAARDFAQKEIAPIAAEFDESGDFPHS
                     TIKKMGELGFMGIEVPEQYGGAGMDPLAYVLALEEICKVDASHGVIMSVNNSLYCHGI
                     LKFGTEEQKKKFITPIASGQAIGAYSLTEPQSGSDAGTMRSRATRDGDFYILNGRKSW
                     VTSGPVANYFVVFMMTDPEKKQKGVTAFLVDGKTPGLTRGKKEPKLGIRASATSELIF
                     ENCRVPAENRLGAEGEGFKIAMTVLDAGRIGIATQALGIAEAAYEAARQYAGQREAFG
                     QPIGQFQGTGFKLADMKTRIEASRLLIYNAALAKEKAKESDGRYSLEASMAKLFASET
                     AMYVTHQAVQIHGGMGYSKELPVERYFRDAKITEIYEGTSEIQRLVISRLELGSK"
     gene            1326..2357
                     /locus_tag="PLT08_13170"
     CDS             1326..2357
                     /locus_tag="PLT08_13170"
                     /inference="COORDINATES: protein motif:HMM:NF024989.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HNE05469.1"
                     /translation="MKAILFHQHGGPEVLAYSDFPTPQPKPGEALVRLRAAALNRVDV
                     FVRNGWPGLKLELPHIPGADGAGEIEEVGEGVSDFKRGDRVVINANLGCGDCEACQAG
                     KDNLCKNWHLLGETICGTYCEYISVPVRQLHKVPPEFDLHKAAAAALVYQTAWHSMVT
                     RGGVKAGETVAIVGAGGGVNSASIQIAKYLGANVIVIGSNTDKLKQAEAIGADILIDR
                     SKDEDWAKAVFLATGKKGVDAIVDNVGATFPLSLRALAKGGRLLTVGNSAAPKFEIDN
                     RFIFAKHLSILGSTMSSISEFREVMDLVFAGKLKPILDKTFPLKEAASAQERLWRGEN
                     FGKITLEIS"
     gene            2357..>2571
                     /locus_tag="PLT08_13175"
     CDS             2357..>2571
                     /locus_tag="PLT08_13175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE05470.1"
                     /translation="MKNFLKRISPFEIILVVAILSVHLIAAQADPYAFPNNWFVRDDA
                     YYYFKVAQNIAEGHGSTFDGINITNGY"
BASE COUNT          727 a          598 c          626 g          620 t
ORIGIN      
        1 taggtcatta ggaaagaatt ccccacatcc aatgacaggg atacggggat taggctcata
       61 ttatagttaa gagcaagatt aaacgtcaat cacaaatttc cgaataatac aaagggtata
      121 attcagaaat accattcgga ggcttttaga tgactgatct tcccatatac ccacttacaa
      181 atgaacataa aatgctccgc gatgctgccc gcgattttgc gcaaaaagaa atcgccccaa
      241 ttgcagccga attcgatgaa agcggagatt ttccacacag caccatcaaa aagatgggcg
      301 aattgggttt catgggtatt gaagtgccag aacaatatgg aggcgcaggc atggaccctc
      361 tcgcctatgt cctcgccctc gaagaaatct gcaaagtaga tgcctctcac ggcgtgatta
      421 tgtcagtcaa taattccttg tattgccatg gcattctaaa gtttggaact gaagaacaga
      481 aaaagaagtt catcacccca atcgcttctg gacaagctat cggcgcttat tctttgacag
      541 aaccccaaag cggctcagac gctgggacga tgcgcagccg tgcgactcgt gatggtgatt
      601 tctatatttt gaatggacgc aaatcctggg tcaccagcgg acctgtggcg aattacttcg
      661 tcgtgttcat gatgaccgat cctgagaaaa aacaaaaagg tgtaacagcc tttctcgtag
      721 atggcaaaac acccggtctg acacgcggaa agaaggagcc aaaacttggc atccgcgctt
      781 cggcgacgag tgagttgatt ttcgaaaatt gccgtgtccc tgccgagaat aggctcgggg
      841 cagaaggcga aggcttcaaa atcgccatga ccgtattgga cgcggggcgt attggaattg
      901 caacccaagc gctcgggatt gcagaagccg cctacgaagc cgcacgccaa tatgcaggtc
      961 aacgtgaggc gtttggtcaa ccgatcgggc aatttcaagg gacgggcttt aaactcgcag
     1021 atatgaagac ccgcatcgaa gcatctcgtt tattaatcta caatgcggct ctggcgaaag
     1081 aaaaagcgaa agagtcggat ggacgttatt cccttgaagc ttcaatggca aaactgtttg
     1141 cttctgaaac agccatgtat gtaacccatc aagctgtgca aattcatggc ggcatgggct
     1201 atagcaaaga attgccagta gagcgttact tccgcgatgc aaagatcacc gaaatttacg
     1261 aaggcaccag cgaaattcaa cgattggtta tttcgaggct tgaattaggc tctaaataac
     1321 aattcatgaa agcgatcctt ttccaccaac atggcggacc ggaagtcctt gcatattccg
     1381 acttccctac tccgcaaccc aagcctggcg aggcactagt tcgattaaga gctgcggctc
     1441 ttaatcgtgt tgatgttttt gtgcgcaacg gttggcccgg cttaaagctg gaacttccac
     1501 acatccccgg cgctgatggt gcgggtgaaa ttgaagaggt tggcgaagga gtatcagatt
     1561 tcaagcgtgg cgatagagtt gtcatcaacg cgaacctggg ttgcggtgat tgtgaagcct
     1621 gccaagccgg gaaagataat ctctgtaaaa actggcattt gcttggcgag actatttgtg
     1681 gaacatattg tgaatacatt agcgtgcctg ttcgtcaact gcataaagtt ccacccgagt
     1741 tcgacttgca caaagccgct gctgcagctt tggtgtatca aacggcgtgg cattcaatgg
     1801 tcactcgcgg cggagtaaaa gctggcgaaa cagtagccat cgtcggcgca ggcggaggcg
     1861 tgaattccgc atccatacag attgcgaaat atcttggcgc aaacgtgatc gtcattggtt
     1921 ccaacacaga taaactcaaa caagctgaag ccatcggtgc agatattttg atcgaccgct
     1981 caaaagacga agattgggca aaggcagttt ttcttgccac cggcaaaaag ggcgtagatg
     2041 ccattgtaga taatgttggt gctacttttc cgctaagctt gcgcgcactt gcaaaaggcg
     2101 gtcgtttgct gactgttgga aatagcgccg cgccaaaatt tgagatcgac aaccgtttta
     2161 tatttgccaa acacctttca attttaggct cgaccatgag ctccatttca gaattccgcg
     2221 aagtgatgga tttggtcttt gcgggtaaac tcaaacccat tctagataaa accttcccac
     2281 taaaagaagc tgccagcgcg caagaacggt tatggcgcgg cgaaaacttt ggaaagatca
     2341 cactcgaaat ctcctaatga agaattttct aaagcgaatt tccccttttg aaatcatcct
     2401 tgtcgttgct attctaagcg tgcacctgat tgccgcacaa gcagacccat atgcttttcc
     2461 caataattgg ttcgttcgcg acgatgctta ttactatttc aaagtagcgc aaaatatagc
     2521 cgaagggcat ggtagcacat tcgacggaat caatatcacc aacgggtatc a
//
LOCUS       DAOQJE010000547         2569 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_28967_length_2569_cov_4.364757, whole genome shotgun sequence.
ACCESSION   DAOQJE010000547 DAOQJE010000000
VERSION     DAOQJE010000547.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2569)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2569)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2569
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28967_length_2569_cov_4.364757"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..406
                     /locus_tag="PLT08_13180"
     CDS             <1..406
                     /locus_tag="PLT08_13180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE05471.1"
                     /translation="SDTSADDWTLQQKVDAQLEILGSSLEAHPLELVQDKISGAISSI
                     DAVAKIGRRVTVAGVRQTSRRSRTAKGDMMLFLTIEDLQGTLDVILFPDVYRIAKSFL
                     DSNPPLLITGVVEMDRERGEPYLRAEKVVSIA"
     gene            complement(612..809)
                     /locus_tag="PLT08_13185"
     CDS             complement(612..809)
                     /locus_tag="PLT08_13185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE05472.1"
                     /translation="MKSCREKLSSGKPCPNQVDEGQEYCPYHLANQDKKAKNMLTIAG
                     AVLSVMVAGVVTVAKIVAKRT"
     gene            complement(812..1792)
                     /locus_tag="PLT08_13190"
     CDS             complement(812..1792)
                     /locus_tag="PLT08_13190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE05473.1"
                     /translation="MSNNDNLISQPNDEIISHMALQSDVIVFDEEKRQKVIQIMRQIP
                     AFLDAVKKLSQGKTYQAVFSPEVLERIKDGSARLDKKGNGLFSSSIRDVETGYFAGPV
                     DLKEVTPDLFTSLNHLATQQTLADIAGRLEVIDEKITDVLQGQINDRLAEIESGIHIY
                     EQAIVASDPDRRELIRSAIEKLNDGRNKIIRSTDINFIDDLGRDRLGMFFSRKRDIPK
                     HVQLKAETIWKAAHAVIKASRYLVMAYSALNQPDSLRVSLEQVKNEVKVFQDKIREIV
                     RWLPPDNNLHESLTTISRGVLPDITELDDISHKTIVIEFQPKEITPPEGV"
     gene            complement(2108..>2569)
                     /locus_tag="PLT08_13195"
     CDS             complement(2108..>2569)
                     /locus_tag="PLT08_13195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE05474.1"
                     /translation="GNHAALIGEDDTHRLFAEVYADPDRPEVRPQEGYKAILSSMQPG
                     WTLRVLQLFWPDPEPRLEFQKQVQRWNTPVTEGLDILHQGLSLAVQEYPLPFVRRTVL
                     EFVLPGDEGVAWWEGLGGLCAGFGLRIRFMDQNAIEGLTRWVLNPNLEYRT"
BASE COUNT          699 a          600 c          544 g          726 t
ORIGIN      
        1 gtcggacaca agcgctgatg attggactct gcaacaaaag gtggatgctc aattggaaat
       61 tctcggctcc agcctggaag cccatcctct tgaactcgtg caggataaga tcagcggagc
      121 catatcctcg atagatgcgg tggcaaagat cggtcggcgc gtgaccgttg ccggagtacg
      181 tcagacctcc cgccgcagtc gaactgccaa gggcgacatg atgctcttcc tgaccattga
      241 agacctgcag gggacattgg atgtgattct gtttccggat gtgtatcgga tcgccaaatc
      301 cttcctagat tcaaatccac ccctgttaat cacaggggtg gtggaaatgg atagggaacg
      361 tggagagccc tatttacgtg cagaaaaagt agtctccatt gcctaatttg acataggaat
      421 taatttactc agtcagaaaa tgctttggat gaacgtagaa ccccaaatgt cagatagtat
      481 ctcgaaaggg ggatatggcg gattgatgtc aggcaggtca gcagagattg ttgtgcagtt
      541 gtcctatatt cagctaaacc gtaaaaattg agaggtttgc atgttttttg gatggcgttg
      601 taattaattt actacgtgcg cttggctaca atttttgcta ctgtaactac accagcgacc
      661 atcacactta ggacagcacc tgcaattgtg agcatatttt tagctttttt atcttggttc
      721 gcaagatgat agggacaata ctcctgtcct tcatctactt gatttggaca aggttttcca
      781 cttgatagct tttccctaca tgacttcatg gtcagactcc ttcgggaggg gtaatctctt
      841 tgggttggaa ttcgatgaca atagttttat gcgaaatgtc gtccaactcg gttatgtccg
      901 gcaaaacacc ccgagaaatg gttgtaaggc tttcgtgcaa attgttatct ggtggaagcc
      961 aacgtacaat ttctcttatt ttatcttgaa atactttgac ttcatttttc acttgttcaa
     1021 gtgatactcg caaggaatca ggttgattca aggcggaata ggccatgaca agataacgcg
     1081 atgctttgat tacagcatga gccgccttcc aaatagtttc agcctttaac tgaacatgtt
     1141 tgggaatatc cctttttcgg ctaaaaaaca tacccagtct atctcgcccc aaatcatcta
     1201 taaaattgat gtcagtagat cttattattt tgtttcgccc gtcattcagt ttttcaatcg
     1261 cactacgtat taattcacgt ctgtccgggt cagaagcgac tatggcttgc tcgtaaatat
     1321 ggatgccact ttcaatttct gccagacgat cattgatttg accttgtaaa acgtcggtaa
     1381 ttttttcgtc aatgacttca aggcgtccag caatgtctgc caaagtttgc tgtgttgcca
     1441 aatggtttaa cgaagtgaac aaatcgggcg tgacttcctt caaatcaacg ggtccagcaa
     1501 aatagcctgt ctcaacatcc cgaatcgatg aactgaaaag accattacct ttcttgtcta
     1561 atctagcaga gccatctttt attcgttcca aaacctctgg agaaaaaaca gcctgatagg
     1621 tttttccttg tgataacttt ttcacggcat ccaaaaaagc gggaatttga cgcattattt
     1681 ggattacctt ctgacgtttt tcttcatcaa aaactatcac atctgactgt aaagccatat
     1741 gtgaaataat ttcatcattt ggttgggaaa ttaagttatc gttgtttgac attagagttt
     1801 ataggccttt atggataaat ccacccagct acaccttgac cgaaccgttc accttctatc
     1861 gcctatgggg tgtcatatcg aatagctcgt tacaacatac tttgtgcaga cagaataaat
     1921 tatatgtcat gcctgccaaa agtggcaata aagtcataga tcagaatcgc catcaagtca
     1981 aagaaaaacg ctcagtccaa tctcaagaag caatctaaat caaattgtct aatgttcttc
     2041 aaaatgggat tatgccttca actctcttct gaattaaatt gataatccct ggagcagact
     2101 actcaaacta tgtcctatat tccaaattcg gattgagcac ccagcgggtc aagccttcaa
     2161 tcgcattttg atccataaat cggatccgca acccgaagcc ggcacacaat ccacccaaac
     2221 cctcccacca agcgacgccc tcatcgcctg gcaacacaaa ttccaatact gttcgccgca
     2281 caaagggtag tggatattcc tgcacagcaa gggataaccc ctgatgcaga atatccaagc
     2341 cctctgtaac aggagtgttc catcgttgaa cctgcttctg gaactccagc ctcggctcgg
     2401 ggtctggcca gaacaactgc agaacgcgca gtgtccagcc cggctgcata gaagacaaga
     2461 tcgccttgta tccctcctgc ggtcgtacct cggggcgatc cgggtcggca tagacttcgg
     2521 caaataatcg gtgtgtatca tcctctccga tcaaagccgc gtggttgcc
//
LOCUS       DAOQJE010000548         2561 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_29118_length_2561_cov_5.052275, whole genome shotgun sequence.
ACCESSION   DAOQJE010000548 DAOQJE010000000
VERSION     DAOQJE010000548.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2561)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2561)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2561
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29118_length_2561_cov_5.052275"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(130..684)
                     /locus_tag="PLT08_13200"
     CDS             complement(130..684)
                     /locus_tag="PLT08_13200"
                     /inference="COORDINATES: protein motif:HMM:NF024273.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HNE05475.1"
                     /translation="MISSDELNPLILSAFTKQITWLEASQKYSLDEFIDTFLNTRAHT
                     LELLDGLTDAQAAFASTVHPFWSISESITHLVYSQGFYHNQLLDIATSQLPHVVEAAR
                     GFGEGAQQHVSVQDLKVSLNAATERIHTVIDSTRNSHDPNKTDASSAFGLCTYKTWIL
                     LLLAHEVDHLRQIAAMRTVAKTNA"
     gene            790..1731
                     /locus_tag="PLT08_13205"
     CDS             790..1731
                     /locus_tag="PLT08_13205"
                     /inference="COORDINATES: protein motif:HMM:NF027484.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NBR1-Ig-like domain-containing protein"
                     /protein_id="HNE05476.1"
                     /translation="MKLTTNNIIIIFGSLILCMLALIPITVLAMPLLETNQPDTQATM
                     QAMVTQTVIALTLNAPTQTPLPPTATPVPATNTPVPTVTTLPTVSYCDWMAFVTDISV
                     PDGTNFSAGETFVKTWRIKNRGTCTWTPDYTLVFNGGDQLGSTTSVRLPGYVSPGQYI
                     DVSITFTAPNNPGSYRGYWIMRNTSGALFGTGDKANVPIYVDIRVRQSDPLPHGTISG
                     NLCYPSEFNPRMTLYIENANTGERIQFAITENQYVYEVLVPAGRYYAYAWAPSYSLEG
                     AYAHDNGLMKSFFVESGKETPFINLCNWGPYGHAKGE"
     gene            complement(1782..>2561)
                     /locus_tag="PLT08_13210"
     CDS             complement(1782..>2561)
                     /locus_tag="PLT08_13210"
                     /inference="COORDINATES: protein motif:HMM:NF013799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HNE05477.1"
                     /translation="RGVFYPALKEMGVEAFNYGLRDFTPSTYSKTVMQGSEIISFKTL
                     SEAFINVKVRLEKQTSPTYIHLYFDKIDAVAHEYGPNSAQAEAEIETFLLMMENYFDQ
                     IFSSKKRVLFLMTADHGMCEVDPKTTVYLNTHKDFSGVEKFIRSNKRGHLIVPAGSAR
                     DMFMYIKNDMVDEAQDFFAKRLEGKADVVKTETLISEGYFGTEISQRFLERVGNVVIL
                     PYRYESVWWYEKDKFEQRFRGHHGGLTPQEMETVLYSMEIG"
BASE COUNT          658 a          609 c          614 g          680 t
ORIGIN      
        1 ggtgacgccg tggacccacg caagaagttc atccaaaccc atgccaagag cgtaagaaat
       61 ttggatattt agtttagttg aataaaaaaa acagcccgcg aattcgcggg ctgttttttt
      121 tattcatgtt taggcgtttg tctttgctac tgttctcatc gccgcgatct gacggagatg
      181 atccacttca tgtgccagca aaagcagaat ccatgttttg taagtgcata acccaaatgc
      241 cgagctggca tctgttttat tggggtcatg tgaattgcga gtcgaatcga tcaccgtgtg
      301 gatccgctcg gtagcggcat tcaggctcac ctttaagtct tgaaccgaaa catgttgttg
      361 ggcgccttct ccaaatccac gggcggcttc tacaacatgc ggcaactgac tggtggcaat
      421 atccaacaat tggttgtgat aaaacccctg cgagtacacc aagtgtgtaa tggattcgct
      481 gatgctccag aaagggtgaa cggtagatgc aaaagccgct tgcgcgtcgg ttagcccatc
      541 gagcaactcc aatgtatggg cgcgggtgtt gaggaaggta tcgataaatt catccaaaga
      601 atatttttga gaggcttcca gccaagtgat ttgcttggta aatgcggaaa gaataagagg
      661 gtttaattca tcggaggaga tcataggttg gattttatct tatttttttt agtactttgg
      721 tactggaaat gatgggaaca atttgtgccg ggcaaacgtt ctaggtgggc aaatgaaaaa
      781 gaggacttta tgaaacttac gaccaataac atcattatta ttttcggtag cctgatctta
      841 tgtatgctgg cgctgattcc gattaccgtg cttgccatgc ctttgttaga gacaaaccaa
      901 ccagacacgc aagccacgat gcaggcgatg gtgacccaaa ccgtgattgc cttaacgctt
      961 aacgccccga cacaaacccc gcttcctcct acggcgacgc ctgttcctgc cacgaataca
     1021 cccgtgccta cggtgacaac ccttcctacc gtcagctact gtgactggat ggcattcgtc
     1081 acagacatct ctgtacccga cgggacgaat ttttctgccg gcgagacctt tgtcaaaaca
     1141 tggcgcatca agaatcgcgg cacatgcact tggacgccag attacaccct tgtgttcaac
     1201 ggcggtgacc agttaggttc cacaacttcg gtgcgtttgc ctgggtatgt atctccgggg
     1261 caatacattg atgtgtccat tacattcaca gcgccaaata accccggctc gtatcgcgga
     1321 tattggatca tgcgcaacac atctggcgcg ctcttcggca ccggtgataa agccaatgtc
     1381 cccatctatg tggacattcg tgtgcgtcag agtgacccgc tgcctcatgg cactattagt
     1441 ggcaacctat gttacccgag tgagtttaat ccgcgcatga cgctttacat cgagaatgct
     1501 aatacaggcg agcgcatcca gttcgcgatc actgagaacc agtatgtgta cgaagtgcta
     1561 gtgcctgccg gacgctacta cgcttatgct tgggctccga gctatagctt ggaaggagca
     1621 tatgctcacg ataatgggct gatgaaatcc ttctttgtgg aaagcggcaa ggaaacaccg
     1681 tttatcaacc tgtgtaactg gggtccgtac ggacacgcga aaggcgaata aaaatcaaaa
     1741 aagcgacctg taatgggtcg cttttttgat tgagtatatt gttatccaat ttccatggaa
     1801 tacaagaccg tttccatctc ttgcggagtt aacccgccgt ggtgcccacg aaagcgctgc
     1861 tcaaatttat ctttctcata ccaccatact gattcatagc gatacggcaa aatgaccaca
     1921 ttcccaactc gctctaaaaa tcgctgagag atctctgtcc caaagtaccc ttcagagatg
     1981 agggtttcgg tcttgaccac atcggctttg ccttccaaac gctttgcaaa aaagtcttga
     2041 gcttcatcta ccatatcgtt cttgatatac atgaacatat cgcgtgcaga ccctgccggg
     2101 acgatcaaat gtccgcgttt gtttgagcgg ataaactttt cgacaccaga gaaatctttg
     2161 tgagtgttga gatacaccgt ggttttagga tccacttcac acatgccatg atcggcagtc
     2221 atcaaaaaaa ggacgcgttt cttgctgctg aatatttggt cgaagtaatt ctccatcatc
     2281 agcaggaagg tttcgatctc ggcttcagct tgagcggagt tgggtccgta ttcatgagcg
     2341 acagcgtcaa tcttgtcaaa ataaagatgg atgtatgtgg gggatgtctg tttttcgagc
     2401 cgtaccttaa cgttgatgaa tgcctcagaa agagtcttga acgaaataat ctctgagcct
     2461 tgcatcacgg tcttggagta tgtagagggt gtgaaatcac gcaagccata attgaaggct
     2521 tccacgccca tttcttttag cgctgggtaa aacacgcctc t
//
LOCUS       DAOQJE010000549         2559 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_29163_length_2559_cov_3.149760, whole genome shotgun sequence.
ACCESSION   DAOQJE010000549 DAOQJE010000000
VERSION     DAOQJE010000549.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2559)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2559)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2559
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29163_length_2559_cov_3.149760"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(73..354)
                     /locus_tag="PLT08_13215"
     CDS             complement(73..354)
                     /locus_tag="PLT08_13215"
                     /inference="COORDINATES: protein motif:HMM:NF016351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF503 domain-containing protein"
                     /protein_id="HNE05478.1"
                     /translation="MIATLTLHLRLPLCTSLKDKRGRIKPLMSRLHREFNVSVAEMDK
                     HDTWDEAVIVCAMVGNDHAFLQSALQTVAKWVEANWSDGDVWDTKIEMV"
     gene            complement(358..1209)
                     /locus_tag="PLT08_13220"
     CDS             complement(358..1209)
                     /locus_tag="PLT08_13220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE05479.1"
                     /translation="MKPNKLFLNLPKSFWASIRLISQEVGYTERSTGQIKVPSNNEIL
                     ESLRSLGIDYSNLQRQNTNSGSFGKHLSEYFSYRANVLNNYVEPRLMDAKKAEKTFNS
                     IFKKLRPSCPIPMNKQKGLMKKPAYLTGLVNMLIEANSNDLPCDYDPHQLTTITRDNL
                     PVRTFARRVDGAFPNSINPIAVWEVKEYYYTTTFGSRVADGIYETLLDGLEIEELREH
                     ENLDVKHYLIVDAYDTWWKQGKSYLCRIIDMLHMSYIDEALFGYEVIERMPEIVKEWV
                     EIYQSTN"
     gene            complement(1196..1447)
                     /locus_tag="PLT08_13225"
     CDS             complement(1196..1447)
                     /locus_tag="PLT08_13225"
                     /inference="COORDINATES: protein motif:HMM:NF024252.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HNE05480.1"
                     /translation="MKILNEKNQREKLLLLLKELRIKKDITQVELAKKLGVPQSFVSK
                     YESGERQLDILELRQICHQIGISLESFIHKLEEKINETK"
     gene            1517..2422
                     /locus_tag="PLT08_13230"
     CDS             1517..2422
                     /locus_tag="PLT08_13230"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: protein motif:HMM:TIGR00571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009007 - site-specific
                     DNA-methyltransferase (adenine-specific) activity
                     [Evidence IEA];
                     GO_process: GO:0006306 - DNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Dam family site-specific
                     DNA-(adenine-N6)-methyltransferase"
                     /protein_id="HNE05481.1"
                     /translation="MKSVKDQSQLYLFETTPNKKRHVEPFNTALLKWVGSKQRMAHEI
                     ISFFPVEFGTYFEPFLGSGGVLGTLVPRKAIASDTFFPLIEIWQTLKSSPDTVKQWYR
                     ERWEKMSKGKKVEAYEKIKASYNAKPNGPDMLFLTRSAYGGVIRFRKADGYMSTPCGV
                     HTPISPDSFSRRVDEWHKRVSGTTFLHLDYEEAMNRAKPGDLIYCDPPYTYSQAILYG
                     AQTFSLEHLLSVIEKCKKKGVYVALSIDGTKRSGNLICDLPLPKGLFKREMMIDIGHS
                     MLKRFQMNGKTLEGERVADRLLLTY"
BASE COUNT          726 a          489 c          496 g          848 t
ORIGIN      
        1 gctcttgttc tttgcggcga agaatctcta cgattttccc agagaccctt cgcctggcgg
       61 ctcagggtga cactacacca tctcgatctt cgtatcccaa acatccccat cggaccaatt
      121 cgcttcgacc cattttgcca cagtttgtaa tgcagactgt aaaaaggcat ggtcatttcc
      181 caccattgca cagacgataa cagcttcatc ccaagtatcg tgtttgtcca tctctgcgac
      241 agagacgttg aactcacggt gcagacgtga catcagcggc ttgatccgtc cgcgtttgtc
      301 tttgagtgag gtgcatagtg ggaggcggag gtgaagggtg agggttgcaa tcatgaatta
      361 gtttgttgat tgatagattt caacccactc ttttactatt tcaggcattc tttctatgac
      421 ttcatatcca aaaagagctt cgtcaatata gctcatatgt aacatatcta taatacgaca
      481 aagataagat ttcccttgtt tccaccatgt gtcatatgca tcaacaatga ggtaatgttt
      541 aacatccaag ttttcgtgtt cgcgaagttc ttctatttcc agaccatcta atagtgtttc
      601 atatatacca tctgcaacac gactaccaaa agtagttgtg taataatatt ctttgacctc
      661 ccaaacagct ataggattaa tgctgtttgg aaatgctcca tccacccggc gtgcaaaggt
      721 tcttacaggt aaattatctc ttgtaatagt tgttagttga tgtgggtcgt aatcgcatgg
      781 caaatcattt gaatttgctt ctattagcat attcaccaac cccgttagat aggcgggctt
      841 tttcattaaa cccttctgtt tgttcattgg gattgggcag gaaggtctta acttcttaaa
      901 tatactgttg aatgttttct cagccttttt tgcgtccatc aatcttggtt caacataatt
      961 gtttaagaca tttgcccggt aggagaaata ttcacttaga tgttttccaa aacttcccga
     1021 gtttgtgttt tgtctttgta gattggaata gtcgattccc aaactcctaa gactttccaa
     1081 tatttcgtta ttcgatggaa cttttatctg acctgtagat ctttctgtgt acccaacttc
     1141 ttgactaatt aaccgaatac ttgcccaaaa acttttcgga agattcagga acaatttatt
     1201 tggtttcatt tattttttcc tccaacttat gaataaaact ttcaagagag atgccaatct
     1261 ggtgacaaat ttgacgtagc tctaggatgt ctaattgacg ttcgccagat tcgtatttgc
     1321 taacaaatga ttgagggacg cctagcttct ttgctaattc aacttgagtt atgtctttct
     1381 tgattcttag ctctttcagt aaaagcaata gcttttctcg ttgatttttt tcatttagga
     1441 ttttcatagt gtgcctgttt gacaaatatc ctaaaaaagg atattattcc ttttagaaag
     1501 atggtatatt cagcccatga aatcagtaaa agatcaatcg caactttatc tatttgaaac
     1561 tacacctaat aaaaaacgtc atgtagagcc atttaataca gccttattaa aatgggtggg
     1621 tagtaaacaa aggatggcgc atgaaattat ttcctttttt ccagttgaat ttggaactta
     1681 ctttgaacca tttctgggaa gtggtggagt tttaggaact ttagttcctc gtaaagcaat
     1741 tgcttcagat acttttttcc ctttaataga aatttggcag actttaaaaa gttcaccaga
     1801 cactgttaaa caatggtatc gagagcgttg ggagaaaatg tcaaaaggta agaaagttga
     1861 agcctatgaa aaaattaagg cttcatacaa cgcaaagcca aatggtccag atatgctttt
     1921 cttgacaaga tctgcttatg gtggcgttat tcgtttccgt aaagccgatg gttatatgtc
     1981 tacgccttgc ggtgttcata ctcctatctc acctgatagt ttttctcgtc gagttgatga
     2041 atggcataaa agagtttccg gcactacctt tttacattta gactatgaag aagcaatgaa
     2101 tagggcaaaa ccaggggact taatttattg cgaccctcct tatacctata gccaagcaat
     2161 attgtatggg gcacaaactt tcagtttgga gcatttatta agtgtaatcg aaaaatgcaa
     2221 gaagaaaggg gtgtatgttg ccttgagcat cgatggaaca aaacggtctg gcaatcttat
     2281 ttgtgattta ccgcttccca aaggtctttt taagagggaa atgatgattg atattggaca
     2341 ttcaatgttg aagagatttc aaatgaatgg aaaaaccttg gaaggtgaga gagtagcaga
     2401 cagattatta ctgacttatt agattactgc ttggttaagt agtggggata tacatgcgtt
     2461 tgtttttgaa tttaccaatt tcgaaatccg tcttacaatc ccatgcatgg atatagaaac
     2521 ccgatacaac caagctcttg attacctgta ttccttcgt
//
LOCUS       DAOQJE010000550         2557 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_29186_length_2557_cov_4.652278, whole genome shotgun sequence.
ACCESSION   DAOQJE010000550 DAOQJE010000000
VERSION     DAOQJE010000550.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2557)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2557)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2557
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29186_length_2557_cov_4.652278"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..749
                     /locus_tag="PLT08_13235"
     CDS             <1..749
                     /locus_tag="PLT08_13235"
                     /inference="COORDINATES: protein motif:HMM:NF014654.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HNE05482.1"
                     /translation="GTYKVQTPVYEGPLDLLLSLIERAELDITTVSLASVTDQYLEHL
                     RNVDQQKPDEISSFLVIATKLVQIKSEALLPRPPEREPGKEDPGEALVEQLKLYKRYK
                     EIAVWMEERQTKNLRTFLRVAPPPKVEPKLDLSNLTLEKLLAAAESAFAKGKEKKPLG
                     TVITAPKVTIREKIDYITKTLKNIQRMSFSGLITDKATRVEIVVTFLALLELVKRYRV
                     TAKQDVLFGDIEFERSEDWKDDEEIEIEFE"
     gene            complement(796..2190)
                     /locus_tag="PLT08_13240"
     CDS             complement(796..2190)
                     /locus_tag="PLT08_13240"
                     /inference="COORDINATES: protein motif:HMM:NF013739.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HNE05483.1"
                     /translation="MKSKFTRRDFLKVASIGTASAIALSCRVLTPSEATISAPTGTST
                     NADVLILGAGIAGLAAARALAEQGRSVIILEARNRVGGRMWTDSSLGLPLDLGASWIH
                     GVTGNPITKLAKEFGVKTVPTDNENGIMFHADGSPMSDAEWASVETLFEEIYGEVATM
                     QEDTDNDMSLQEAFNEVLSEYDLSEEELRRLNFYAYQGTALEYGADMKDLSLWWWDQD
                     EEFGGEEVIFPDGYNQITDGLAQGLDIRLGTVVNTVRYGTDEVEVETSAGVYVADQAV
                     VTFPLGVLKQASVKFEPPLPEFKQAAIDRLAMGVLNKVYLKFSEAFWGEDIETISYLG
                     EELGEWCDWLSFVPFTGEPVLMAFHGGDKGFALEELSDDEIVAGAMQTLRTMFGSDIP
                     EPEGVLITRWGQDPFSFGAYSHIPPFASGDDYDALAVPVDDVLFFAGEATSREYPSTV
                     HGAYLSGLRAAEEM"
     gene            complement(2187..>2557)
                     /locus_tag="PLT08_13245"
     CDS             complement(2187..>2557)
                     /locus_tag="PLT08_13245"
                     /inference="COORDINATES: protein motif:HMM:NF016763.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HNE05484.1"
                     /translation="LDIVPRWFPAFLMMAIIFAFSSRAKDELPNFQDWEYFVKKSSHF
                     LVYGLLALSYIRALPKRNHLLAWALAILYALSDEFHQSFVPGRTSSLVDVFVFDNLGA
                     IVALVLHQYLTIKNNRKETQ"
BASE COUNT          744 a          652 c          533 g          628 t
ORIGIN      
        1 ttggcacgta caaggttcag accccagtgt atgagggtcc gcttgatctt ttgttaagcc
       61 tgatcgaacg tgccgaattg gatattacaa ctgtttcact tgcttcggtg acagaccaat
      121 atcttgaaca cctccgcaat gtagatcaac aaaagccaga cgaaatttcg tcttttcttg
      181 tgatcgccac caagttggtg caaatcaaat cggaagcgtt gttaccacga ccacccgagc
      241 gcgaacccgg taaagaagac cctggcgaag cacttgtgga gcagctaaag ctttacaaac
      301 gctacaaaga aatagccgtt tggatggaag aacgtcagac gaaaaactta cggacgttct
      361 tgcgtgtggc accgcctccg aaagtggaac cgaagctgga cctttccaat ttgaccttag
      421 agaagctgtt agcagcagcg gaatcagcgt ttgcaaaggg caaagagaag aaaccgctcg
      481 gaacagttat cactgcgccc aaagtaacca ttcgcgaaaa gattgattac atcaccaaga
      541 ccttgaagaa cattcagcgc atgagcttta gtggattgat taccgacaaa gccactcgcg
      601 ttgaaattgt agtgaccttt cttgcattgc tagagttggt caagcgttac cgcgtcacag
      661 ccaagcaaga tgtgttgttc ggcgatattg aattcgaacg cagcgaagat tggaaagacg
      721 acgaagaaat cgaaattgag tttgagtaat attaatcaaa agcggatgag gttttgcctc
      781 atccgctttt attttttaca tctcttccgc agctcgtagc cctgagagat atgccccatg
      841 aaccgtgctg ggatactctc gtgaagtcgc ttcgcccgcg aagaacaaca catcatctac
      901 tggtaccgca agtgcatcat aatcatctcc actcgcaaaa ggcggaatat gcgaatatgc
      961 accaaacgaa aacgggtctt gaccccagcg agtaatcaaa acaccttcgg gttcagggat
     1021 atcactccca aacatcgtcc gcaaggtttg catagcgccc gcaacaatct catcatcgct
     1081 caactcttca agcgcaaaac ctttgtcgcc gccatgaaat gccattaaca caggctcacc
     1141 tgtaaatggc acaaaactta accagtcaca ccactcgcca agctcctctc ctaaataact
     1201 gatcgtctct atatcctcac cccaaaacgc ttcggagaac ttcaaataga ctttgttgag
     1261 gacgcccatc gcgagccgat caatcgctgc ttgtttaaac tcaggaagtg gcggctcaaa
     1321 cttaaccgat gcctgcttca acaccccaag tggaaacgtt acaacagctt gatctgccac
     1381 ataaacccca gccgatgttt ctacctccac ctcatctgtc ccatatcgga ctgtattgac
     1441 taccgtacct aaacggatat ccagtccttg ggcgagtcca tctgtaattt ggttatatcc
     1501 atcggggaaa attacttcct cgcctccaaa ctcttcgtct tggtcccacc accaaaggga
     1561 taagtctttc atatcagcac cgtattcaag tgcagtgcct tggtaggcat aaaaatttaa
     1621 gcggcgcaac tcttcttcag ataagtcata ttctgaaagc acttcattaa atgcttcctg
     1681 taacgacata tcattatcgg tatcttcttg catcgtagcg acttcgccat aaatttcttc
     1741 aaataaagtt tcaacactcg cccattccgc atccgacatt ggactcccgt ctgcatggaa
     1801 cataatgcca ttctcgttgt cagttgggac ggtctttacg ccaaactctt tcgctagttt
     1861 tgtgattgga ttgccagtca cgccatgaat ccaagatgcg cctaaatcca atggcaaccc
     1921 cagcgaggaa tcagtccaca tccgcccacc gactcgattt cttgcttcga gaataatgac
     1981 actacgtcct tgttcggcga gagctcgagc cgcggcaaga ccggcaatcc ctgctccaag
     2041 aatcagaaca tctgcattgg tagaagtgcc agttggagct gaaatagtcg cttcgcttgg
     2101 cgttaagacc ctgcaagaca gcgcaattgc agatgcggtg ccgatggaag ctactttcaa
     2161 aaaatctcgg cgggtaaatt tagatttcat tgtgtctctt ttcggttatt ttttattgtt
     2221 aagtactgat gaagcactaa agcaacaatt gcaccaaggt tatcaaagac aaacacatct
     2281 accaaagacg acgtccgccc ggggacgaaa gactgatgaa actcatcgct caaggcatat
     2341 aaaatcgcca gcgcccacgc tagaaggtga tttcgctttg gtaaagctcg aatataggag
     2401 agtgcaagca atccatatac caaaaaatgg gatgattttt ttacaaaata ctcccaatct
     2461 tgaaaattag gcagttcatc tttcgctctg gaagaaaaag caaaaataat cgccatcatt
     2521 aaaaaggcgg gaaaccatcg cggtacaata tcaagaa
//
LOCUS       DAOQJE010000551         2556 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_29207_length_2556_cov_6.645342, whole genome shotgun sequence.
ACCESSION   DAOQJE010000551 DAOQJE010000000
VERSION     DAOQJE010000551.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2556)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2556)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2556
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29207_length_2556_cov_6.645342"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            265..825
                     /locus_tag="PLT08_13250"
     CDS             265..825
                     /locus_tag="PLT08_13250"
                     /inference="COORDINATES: protein motif:HMM:NF012783.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease proteolytic subunit"
                     /protein_id="HNE05485.1"
                     /translation="MSNKTVYINFYDGINDKTVKILMAICSDVIAQHKPDILYFLFAS
                     GGGWVTAGITFHNFLRSLPVKVVMHNMGVIDSIATVIFLAGEERFTSPHSSFLFHGVE
                     WTFAQQTSLSTSKLAEHSSQLKQDERKIANVYLERTALTDTEITELFKQGESKDPTFA
                     LEKKFIQEIKNPAVPKDAILISVNIN"
     gene            1060..>2556
                     /locus_tag="PLT08_13255"
     CDS             1060..>2556
                     /locus_tag="PLT08_13255"
                     /inference="COORDINATES: protein motif:HMM:NF015338.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HNE05486.1"
                     /translation="MRRLFILSAFFLFVLTSCGVPSTITNLPTKTPTELPSPTVSPTP
                     KPLLTVKVMSYNILWGAGVDREFDENLKAKGQDSQYGRDRLPELLSVIKELDPDILGI
                     QEGAGWDRGTPSVIQKVAEELNMNYFLAKGAASELNVMILSKFKIIETENLSPEVGNV
                     GALRVKLTTPDGQPLNVFVVHLDPFSANIRLCEINTLIQYMQPYFQQRTIVMGDFNTT
                     PNLWDHKNLKETGMTLVAIEQSWQIDQIWASPSMNWSKTDWFKSFLPPKNISDHHPIG
                     AEIAIFSNPISLPTVTPIPPTPVIKPIPLIYDFITDPQVLRFDNIEDACTKPKWNSDW
                     TTEKFSDGVIAIVGEDYWKAGVSRNKDFSEGQGVVLRFKFEKGTEANIFFDSGDWSTN
                     PYRRFGMNIRQDSVQTDLWQGKTGMGGKNLNGNFKPKPETWYNLMIVISKNGEFLEAV
                     WEPNDPSQVVTYHEQLGEKWTGLTWRFSVGANTGNVLIDDFSEIIFDKI"
BASE COUNT          816 a          500 c          542 g          698 t
ORIGIN      
        1 aggccgtctt cgccgcctac ggctgggaga acaccctgtc cgatgaagag atcctcgaga
       61 gattgctggc cttgaatttg gagagagcga agtaaagtat ccaacgggac ggtcacccgc
      121 agtccgcaag acggctggaa atgtccggtt tggagagacg gttgtataat cattttatgg
      181 agtctatagg gcgcttttac agcgcggcgc tgtatatact atagttagcc cgcctttcta
      241 tcattcaaat aaagggagat ttgtatgtcg aataaaactg tttacataaa tttctacgac
      301 gggataaatg ataaaaccgt aaagatacta atggcaattt gctcagatgt aattgctcaa
      361 cacaagcctg atattttata ttttctcttt gcgtcaggtg gtggttgggt aacagcagga
      421 attacttttc ataatttttt gcgttcatta cctgtaaagg ttgttatgca caacatggga
      481 gtaattgact cgattgcaac tgtgatattt ttagcaggcg aagaaagatt tacttcacct
      541 cattcttctt ttctttttca tggtgttgag tggacatttg cacaacaaac ttctctctca
      601 acaagtaaac ttgccgagca ttcaagtcaa cttaagcaag atgaaaggaa aattgcaaac
      661 gtatatttag aacgcacggc acttacagat acagaaataa ccgaattgtt caaacaaggc
      721 gagtcgaaag accctacttt tgcactggag aaaaaattta tacaagaaat taaaaatcca
      781 gccgtaccta aagacgcaat tcttatttcg gtaaatataa attagtcttt caagaatcgg
      841 cgggctaaca aagcgtgcag caactgtctt gaaagtcaag agcaaaacca cctttaaagg
      901 gaaacccaga ccgccttagg tcatgttggc tggacggctt cgcccggtcc gcgagacagg
      961 gcaggtaccc cctacgggga cgatggcgca atccgttggg tggctggtcg cacaaacaag
     1021 tatttgttaa tattttcata ttcggtggag aaatctaaaa tgagacgcct tttcattctt
     1081 tcggcattct ttctctttgt tctaacatct tgtggtgttc catctacaat tacaaatcta
     1141 cctacaaaga caccaactga attgccgtca cctacggttt ctccaacgcc aaagccacta
     1201 ctaacggtca aagtgatgtc atataacatt ctttggggtg caggcgttga ccgtgagttt
     1261 gacgagaatt tgaaagccaa aggacaagat tcacaatatg gaagggaccg attaccagaa
     1321 ctattgtcag taatcaaaga gttagaccct gatattctag gcattcagga aggcgctgga
     1381 tgggatagag gcacaccatc tgttatccaa aaagttgccg aagaactcaa tatgaactac
     1441 tttttagcaa aaggtgcggc aagcgaactc aatgtgatga ttctcagcaa attcaaaatt
     1501 attgaaacag agaatttatc accagaagta ggcaatgtag gcgcactacg agttaaattg
     1561 acaactcctg atgggcagcc tttaaatgta ttcgtagtgc atcttgatcc attttcagca
     1621 aacatccgat tatgtgaaat aaatacactt attcaatata tgcaaccata ttttcaacaa
     1681 cgaacaatag taatggggga ctttaatact acaccaaatt tatgggatca taaaaacctg
     1741 aaggaaactg gcatgacact agtagcgatt gaacaatctt ggcagataga tcaaatatgg
     1801 gcatccccct ctatgaattg gtcaaaaaca gattggttta aatcctttct ccctccaaaa
     1861 aatatatctg atcatcatcc tattggcgct gaaattgcta tattttctaa cccaatatcg
     1921 ttaccaacag ttactccaat tccaccgacg ccagttatca agccaatacc attgatttac
     1981 gattttataa ctgatccgca agtgttacgc ttcgacaaca ttgaagacgc ttgtacaaaa
     2041 cccaaatgga attcagattg gacaaccgag aaattttctg atggggtgat tgctatcgta
     2101 ggtgaagatt actggaaagc aggagtctca agaaacaagg atttttcaga aggtcaagga
     2161 gttgtattac gctttaaatt tgagaagggg acagaagcca atatattctt tgacagcggg
     2221 gattggagta caaatccgta tcgaagattt ggaatgaaca ttcgacaaga ttcggttcag
     2281 acggatttat ggcaaggcaa aacaggaatg ggtggcaaaa atcttaatgg gaatttcaaa
     2341 cccaagcctg aaacttggta caacctcatg attgttatat ctaaaaacgg agaatttctg
     2401 gaagcagtct gggaaccaaa tgacccttct caggtagtca cataccacga gcaattgggt
     2461 gaaaagtgga ctggactaac ttggcgattt tcagtaggag caaatacagg caatgtattg
     2521 attgatgatt ttagtgaaat catttttgac aagata
//
LOCUS       DAOQJE010000552         2556 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_29208_length_2556_cov_6.588165, whole genome shotgun sequence.
ACCESSION   DAOQJE010000552 DAOQJE010000000
VERSION     DAOQJE010000552.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2556)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2556)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2556
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29208_length_2556_cov_6.588165"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(742..978)
                     /locus_tag="PLT08_13260"
     CDS             complement(742..978)
                     /locus_tag="PLT08_13260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE05487.1"
                     /translation="MGAIATAIGVAYWYVYKDPIHNIAILKVGIVDNGLVSLVLIIFI
                     VFYNFRNITLLTSLPLTLFFFLSFILLMPKTEAA"
     gene            complement(1450..1752)
                     /locus_tag="PLT08_13265"
     CDS             complement(1450..1752)
                     /locus_tag="PLT08_13265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE05488.1"
                     /translation="MNIYMKSGIVSLISGMLGASLTVAVMDFTFGLDVAINSFFKVLP
                     RSICIGGVPSLILAAVIFAFWKKSGKNEKGLMTICLITGFIVGVLCGVPLYISYGI"
     gene            complement(1893..2201)
                     /locus_tag="PLT08_13270"
     CDS             complement(1893..2201)
                     /locus_tag="PLT08_13270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE05489.1"
                     /translation="MELSRKKEAFTIVTSGFIPVILIALGFSFFTANGACFPMSFFIA
                     GGWLGIFLVSLIFSGYGAFVGWTGFRDVSFNKKLSAKTMALTIIGLLGIFAFLFPVCD
                     "
BASE COUNT          774 a          564 c          524 g          694 t
ORIGIN      
        1 agttttacac acaactgaat ctttggcaac tgacaaaaac atgagacatt attggggtgc
       61 gtgttgtcgt gaaatctcaa attgtcgtgc aatcagaaat aagggaaata ttacactcac
      121 tgcgataagg aacgaaagga gaatgtagac ccaaacatag cgtattctga gccttcgtgc
      181 ttcaacagcc ataaatatac aaccagcaag cgcatacaag aggatgtcgt ttgtgaagga
      241 cgctacggct ggattcgcgt aaagagcatt tctgtaacct atcatgcctc cattctgcgg
      301 ttggaggaaa aacgcaatgt tattaacctg agttgcgatc agtgcgacca ctgcaattat
      361 tgcgtacaca atgcaaagag tcttttcaga acgtgtcatc gaacctcttt aatgatagag
      421 caatagttga acagtggcaa aatcttcaag ccaaaaaaac atgtaaaaga ataccgctaa
      481 aaattgacca aacccaaaga atgaatttgc ttttcaaacg cgtcgataaa acgaaacttg
      541 cgattcaaag acaattttgt atttgctctt ggttgcccaa cggattgcgg cttgcactgt
      601 gagtacctgc cccgtctcgc ggaccgggcg gatctgtcca gcaagagaaa ggcaaaggct
      661 gagtcttccc ctttagggga agatgtccaa aggacagaag aggtcaggtg cgtatcatcc
      721 cggttctatc gggacgcttt gttaggcggc ttctgtcttt ggcatcagca ggatgaagga
      781 gagaaagaag aagagcgtta gtggtaggct ggtcaataag gtaatattcc tgaaattgta
      841 gaaaacaata aaaattataa gcacaagaga gactagccca ttatctacga tccccacctt
      901 caatatcgca atattgtgaa tgggatcttt gtaaacatac cagtatgcga ctccaattgc
      961 cgtggctatg gctcccatga gtctcatata aatgggttga tcaggtattg gtaagccaaa
     1021 ccactgtaat aatgttattg ggtatagaag caatggtaaa cccgagaaga caagagtata
     1081 gatgaccttg acagctagaa tcctttttag ccataatagt ttattactgt tggacatttt
     1141 gtattctcct tgtatagtat ggttcgttga ttccagctaa ccaacggtta acaaaagaca
     1201 gaattctggt attctgagag ttggctcagg gaatgatact gatgttcttc tagtggcatg
     1261 atgtaattta catttgacat atctgccgcc taacggattg cggcttgccc tgtggggacc
     1321 cgccctgtct cgcggaccgg gcgaagccgt ccaaccagcc tgtccggcct gcaccatgcc
     1381 ccagcgtcag gtgcatatca tcccagttct atcggggcgc tttgttagcc cgcttttgtc
     1441 ctttcaggtt tatatgccat aagaaatata aagtggtact ccgcacaaca caccaacaat
     1501 aaagcctgtt attaggcata ttgtcattaa gcctttttca tttttgccag actttttcca
     1561 aaaagcaaag atgaccgccg ctaaaatcaa actcggaact cccccgatac aaatactcct
     1621 ggggagtact ttaaagaaac tgttgatagc aacgtctagc ccaaatgtga aatccataac
     1681 tgcaacagtg agactcgccc caagcattcc tgatattaga ctgacaatac ctgatttcat
     1741 gtaaatgttc atacctcatg aaactccttt ctagcacaat gcctgaaaca cgacttactt
     1801 taaaaattta ctgattgttt tatttagact tccgtttata ttcctttatt tggtattgaa
     1861 tcatcctttg ctgtgctttt catttcattt tatcagtcac aaacaggaaa tagaaaggcg
     1921 aagataccta ataagccaat aatagttaat gccattgttt tagcacttaa tttcttgtta
     1981 aaagaaacgt ccctgaatcc tgtccaccca acaaacgcac cgtaaccgct aaatattaac
     2041 gaaaccaaga aaataccaag ccatcctccc gctatgaaga atgacatagg gaagcaagcg
     2101 ccgtttgctg tgaagaaaga aaaaccaaga gcgattaaga taacagggat gaaaccactt
     2161 gtgactatcg taaacgcttc tttttttcta gataactcca tgatgatttc acctttcaat
     2221 tacagtttat ggccaattgc ggattcataa aaatataaac agaaagaatc atttattttt
     2281 tattctttag agactgaacc tgatttttca ctcagttgtg caaggggcgg gctaactagg
     2341 gtatatacag cgctgcgctg tatataccta tagactccat aaaacgatta tacaaccgtc
     2401 tctccaaacc ggacaattcc agccgtcttg cggactgcag gtgaccgtcc cgttagatac
     2461 cttgcttcgc cctctccaaa ttcaaggcca gcaatctctc caggatctct tcatcggaca
     2521 gggtgttctc ccagccgtag gcggcgaaga cggcct
//
LOCUS       DAOQJE010000553         2556 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_29210_length_2556_cov_4.973611, whole genome shotgun sequence.
ACCESSION   DAOQJE010000553 DAOQJE010000000
VERSION     DAOQJE010000553.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2556)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2556)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2556
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29210_length_2556_cov_4.973611"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..435
                     /locus_tag="PLT08_13275"
     CDS             <1..435
                     /locus_tag="PLT08_13275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE05490.1"
                     /translation="PDKICTPHRNISRIWISWLRIAYPRAEIWTGWSEAHLPGIHVYP
                     DALAWGRIQGYETLFWLEFGDGHKKKEAIKRITRIRLESALKFCQVTGVRLVYAQISP
                     KWVQKAVGAALGPLPPDMAAVMTSPRRFGNLPTVEWGSITMI"
     gene            493..>2556
                     /locus_tag="PLT08_13280"
     CDS             493..>2556
                     /locus_tag="PLT08_13280"
                     /inference="COORDINATES: protein motif:HMM:NF024491.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase D-like domain-containing protein"
                     /protein_id="HNE05491.1"
                     /translation="MIITDNHLKNNSRFFDGKRAALQCISSALTSAEQVRIATAYFEG
                     SGFQALQNVLSGKKVKLLVGREEGGEDSLREVLQEFVNELSYGPMENRTRSMRQMLEA
                     LEQGMMAVSVGASLPQDTPIMDARYIYHHAKLYIADKTTAVVTSANFSYHGLCISREA
                     GITIDKADDVTYFVERFDYYFEKARQITAELIEALRSWLKAYDPYIIYARALLELYGL
                     PQEGAAPQLPPLAKYQEGVVSSVLRSMTEHDGAFLIASTGLGKTVIAAHVAAYLRMQG
                     DIDSALVVCPAGLREVWRRSMRSARVPSIEFSYHTLTRKDGDSNLPILEHELKQVNQQ
                     TLIILDESHRLRNEEANGGDLRLSNDRIQKAAREKSAKVLMLTATPYGKDFSEVESQL
                     RLLPAPKQAQSTALGFTVETQMWQAKQLSDLPELAPCTVLTTPDVVRHFSKQDANGER
                     FVVFSEKDQRYFPRRISLRTIRYQNPFDDFLADLFESRLLYKKIERQENPIQAPLPLA
                     IPFTEGERLALQEALFLHQFCSSPAEVQNVCTQLEKGSYNYSFARQQELTAFIQERRS
                     LIRNAIKPRNDLKLAHLLKIIEEAGNEKIVVFCEYHETARSLRDGLKRLKSVKVETTV
                     DTPDLDDLLRRFAPIANEVLPEERNDEEELQVLIATRSMSEGFNMQDASILVNYDLPW
                     TVLQLA"
BASE COUNT          721 a          578 c          615 g          642 t
ORIGIN      
        1 cctgacaaga tatgtacccc ccacagaaat atttcgcgca tatggatatc ctggttgcgc
       61 atcgcatatc ctcgtgcaga aatttggaca gggtggagtg aagctcattt acctggcatc
      121 catgtatacc cggatgcatt ggcatgggga cgtattcagg gatatgagac cttgttctgg
      181 ctggaatttg gggatgggca taaaaagaag gaagctataa aaagaataac taggatacgg
      241 ctggaatctg ccctaaaatt ctgccaagtt acaggtgttc gccttgtata tgcccaaata
      301 agcccaaagt gggtgcagaa ggctgttggt gcagcacttg gcccattacc accggatatg
      361 gcggcggtga tgacaagtcc acgcagattt ggaaatttgc cgactgtgga gtggggatca
      421 ataaccatga tttgaaaacg gttatactta gcattactgg tcaactccca aggagaatgt
      481 actgggggaa atatgataat taccgacaat catctcaaaa acaattccag gtttttcgat
      541 ggaaaaagag ccgcacttca atgcatttca tctgccttga catcggctga acaggtgcgg
      601 attgcaacag cctactttga aggatcaggg tttcaagcgt tgcagaatgt attaagcggc
      661 aagaaagtca aattattggt tgggcgagaa gaaggcggcg aagacagtct gcgggaagtt
      721 ttgcaggagt ttgtcaacga gctttcctat ggcccaatgg aaaatcgaac acgctccatg
      781 cgccagatgc tggaagcctt ggaacaaggc atgatggcgg tatcggtcgg agcgtccctt
      841 ccacaggata caccaatcat ggacgcccgc tacatctatc atcatgccaa actttatatt
      901 gcggataaaa cgaccgcagt ggttacctcc gcaaattttt cttatcacgg tctttgcata
      961 agccgtgagg cgggaattac cattgataaa gcagatgatg tcacctattt cgtagaacgc
     1021 tttgactatt actttgaaaa agcccgccaa atcactgctg aattgatcga agcgctgcgt
     1081 tcctggttga aggcatacga tccttatatc atttatgcaa gagccttgct ggaattatat
     1141 ggacttccac aagaaggcgc agccccacag ttgcctcctt tggcaaaata tcaggaaggc
     1201 gttgtctcta gtgttcttcg ctccatgaca gagcatgatg gggcgtttct tattgcatct
     1261 acaggactgg ggaagactgt cattgccgcc catgttgcgg cgtatttacg aatgcagggt
     1321 gatattgaca gcgccctggt cgtttgcccg gcagggctgc gggaagtatg gcgccgctcc
     1381 atgcggtctg ccagagttcc gagtatagaa ttttcctatc acaccctgac acgcaaagac
     1441 ggcgactcga acctgcccat ccttgaacat gagctaaagc aagttaatca acaaactctg
     1501 atcattttgg atgaatctca ccgactacga aatgaagaag ccaatggtgg agatttacga
     1561 ttaagcaatg accgcatcca aaaagccgcg cgagaaaaaa gcgcaaaagt attaatgctt
     1621 actgcaacgc cgtacgggaa ggatttctcc gaggtggaaa gccagttgcg gttattgccg
     1681 gcgcccaaac aagcacaaag cactgctctt ggttttacag ttgagactca gatgtggcag
     1741 gcaaaacaac tttctgattt acctgaactt gctccatgca cagtgctaac cacccctgat
     1801 gtcgtccgcc attttagtaa gcaggacgcc aatggagaga ggtttgttgt tttctcggaa
     1861 aaagaccaaa gatattttcc gagacggatt agcctccgaa cgatacgcta tcaaaaccct
     1921 tttgatgact ttctggcaga tcttttcgag agcaggctgc tttacaagaa aattgagcgc
     1981 caggaaaatc caattcaggc accattaccg ttagctattc catttacgga aggggaacgg
     2041 ctagctctcc aagaagccct attccttcat cagttctgtt cttctcctgc agaggttcag
     2101 aatgtttgta ctcaattgga gaagggtagt tacaactatt catttgcaag acagcaggaa
     2161 cttaccgcat ttattcaaga gcgccgatct cttatacgca atgccataaa accccgcaac
     2221 gatctcaaac tcgctcacct tttgaaaatc atcgaagaag cagggaatga aaaaattgtt
     2281 gtgttttgtg aataccacga aacagcccga tctttgagag atggattaaa acggctcaaa
     2341 tctgtaaaag ttgagactac tgtagataca cctgaccttg atgatctatt acgcaggttt
     2401 gcgcccatcg ccaatgaagt cctgcctgaa gaacggaacg atgaagaaga attgcaggtg
     2461 ttaatcgcta ctcgttctat gtccgaaggt ttcaacatgc aagatgcatc gattctcgta
     2521 aattacgatc tgccttggac ggtgctgcaa ttggca
//
LOCUS       DAOQJE010000554         2549 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_29353_length_2549_cov_6.433841, whole genome shotgun sequence.
ACCESSION   DAOQJE010000554 DAOQJE010000000
VERSION     DAOQJE010000554.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2549)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2549)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2549
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29353_length_2549_cov_6.433841"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..256
                     /locus_tag="PLT08_13285"
     CDS             <1..256
                     /locus_tag="PLT08_13285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE05492.1"
                     /translation="WQGVLRLPKHPNVALDHAQRAKALENRVEAVYREAVADLFSGPE
                     DIHHVVEMLKLREVYRHLSNAADRGDEAANVIADIVVKRT"
     gene            558..1742
                     /locus_tag="PLT08_13290"
     CDS             558..1742
                     /locus_tag="PLT08_13290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HNE05493.1"
                     /translation="MRTKNFAYHLLALFVIAATLLAACGAPAPTEAPAAATDAPAATE
                     AAAATEAPAADPMADLVAAAQAEGTLTVIALPHDWCNYGEAIDTFKAKYGLEVNELNP
                     DAGSGDEIEAIKANKDNTGPQAPDVIDVGFAFGESSKAEGLIQPYKVSTWDSIPAEAK
                     DPEGYWYGDYYGVLSFLVNTDVQPDVPQDWADLLDPKYNGQVALSGDPRTSNQAIQTV
                     YAASLANGGSLDDAQAGLDFFAQLNSAGNLVPLISNNGLVAKGETPVRITWDYNALAA
                     VDSFAGNPAAQVVIPATGRFAGVYVQAISAYAPHPNAAKLWMEFLYSDEGQTIWMKGY
                     CHPIREADMRERGVIPADLLAKLPDVSGAVFPSLDQLNAAKELITTNWDSAVGSDIQA
                     AP"
     gene            1824..>2549
                     /locus_tag="PLT08_13295"
     CDS             1824..>2549
                     /locus_tag="PLT08_13295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HNE05494.1"
                     /translation="MAETALPTKKNVAPRFDWKKWLGVTPFFLFALLFLILPSLKLFV
                     GAFTDDAGRFTLANMLALFTQQSILDSYKTSIQISAVTAIGGGFFGFLLAYSITVGGL
                     PRSLRSMLITFSGVASNFAGVPLAFAFVATLGRTGIITAFLDEFVFQKFFGVKLYQTG
                     FNLYSFLGLSLTYMYFQFPLMVLIITPALDGLKREWREAAENLGATQFQYWSKVAMPI
                     LLPPLLGATILLFGNAFGAYATAY"
BASE COUNT          551 a          633 c          618 g          747 t
ORIGIN      
        1 ttggcaaggc gtgctgcgtt tgcctaagca cccaaatgtg gcattggatc atgcgcaacg
       61 tgccaaggca cttgagaacc gtgtagaagc ggtataccgt gaagcagttg ctgatctgtt
      121 tagtggacct gaagatattc accatgtggt tgagatgctt aagctccgtg aagtgtatcg
      181 tcatctttcc aatgcggctg atcgcggcga tgaagctgcc aatgtgattg cggatattgt
      241 tgtaaaaaga acttaaccgg atgttaatcc cttttctttt tgagtttgtt attatccttg
      301 cgctgataat tgaatttagt aatgatgcgc gcaacattca aagtggtaat accgatatct
      361 tgtatgtgac tcccatgttt attggtacaa ataactacgt tggtcattaa ttcaaagcct
      421 cacaagcttg tttgactttg aatattgata atgctaaaat gttaacgatt tgttaatgga
      481 atggaggtgc cgggaagtta agatagcgca agtattccgt tcgttcgatc aatctaaaat
      541 atcataagga gaaagagatg cgtacgaaga attttgctta tcatttgctc gccctgttcg
      601 tgatcgccgc taccttgttg gctgcttgtg gtgctcctgc tcccactgaa gcccctgctg
      661 ctgcgaccga tgcccccgct gccaccgaag ctgctgccgc cactgaagcc cctgctgccg
      721 accccatggc tgacctcgtt gctgctgcgc aggctgaagg aactttgacc gtgattgcct
      781 tgccccatga ctggtgcaat tatggtgaag ccattgacac cttcaaggcg aaatatggtc
      841 tcgaagtcaa tgaactcaat cctgatgctg gctccggtga tgaaatcgaa gctatcaagg
      901 cgaacaaaga caacacgggt cctcaggctc cggatgtgat cgacgttggt tttgctttcg
      961 gtgaatcttc caaggctgaa ggtttgatcc agccgtacaa ggtttccacc tgggattcca
     1021 ttcctgctga agccaaggat cctgaaggct actggtatgg cgactactat ggtgttcttt
     1081 ccttcctcgt caataccgat gtccaacctg atgttcctca ggactgggct gacttgctcg
     1141 atcccaagta caacggacaa gttgctctct ctggcgatcc ccgcacatcc aatcaggcta
     1201 tccagaccgt ctacgctgct tcccttgcga atggcggctc cctcgacgat gcacaggctg
     1261 gtctcgactt ctttgctcag ttgaacagcg ctggtaacct tgttcctttg atctcgaaca
     1321 atggtttggt tgccaagggt gaaacccccg tccgcatcac ctgggactac aatgctctcg
     1381 ctgctgtgga ttcctttgct ggcaaccctg ccgctcaggt tgtgatcccc gccactggtc
     1441 gtttcgctgg tgtgtacgtt caggcgatca gcgcctacgc cccgcacccc aatgccgcca
     1501 agttgtggat ggaattcctg tactctgacg aaggtcagac catctggatg aagggctact
     1561 gccacccgat ccgcgaagct gatatgcgcg aacgcggtgt gatccccgct gacttgcttg
     1621 ccaagttgcc cgatgtttct ggcgctgtgt tcccctccct tgatcagttg aacgctgcaa
     1681 aagaattgat caccaccaac tgggactccg ctgtcggttc cgacattcag gctgctccgt
     1741 aatagttcgt tcttgtaaga aatgcgagac cctgccttat ggcggggtct cgccgcaatt
     1801 gaaccatttt cgaggagttc cctatggcag aaactgctct tcccaccaaa aagaacgttg
     1861 ctccaaggtt cgattggaaa aaatggttgg gggttacacc gtttttcctt tttgcacttt
     1921 tattcttaat tctgccctct cttaaattat ttgttggggc atttaccgat gatgctgggc
     1981 gttttacgct ggcgaatatg ctagcattgt tcacacagca atccattctt gattcctata
     2041 aaacgagcat tcagattagt gcagtcaccg cgattggggg cggctttttc gggttcctgc
     2101 tggcatactc catcactgtg ggtgggttgc cgcgatctct gcgctctatg ttgattacat
     2161 tttcaggcgt tgcatccaac tttgcaggcg tgcctttggc attcgccttt gttgctacgc
     2221 ttggacgtac aggtatcatc actgcttttc tggatgaatt tgtatttcaa aaattttttg
     2281 gcgtaaaact ctaccaaacc ggtttcaatc tatatagctt cctcggtttg agtctcactt
     2341 acatgtattt tcaatttcca ttgatggttt tgatcattac cccggcattg gatggtttga
     2401 aacgtgaatg gcgtgaagct gccgaaaatt tgggcgcgac ccaatttcaa tattggtcaa
     2461 aggttgccat gccgatatta cttccgccat tgttgggtgc aaccattttg ttgttcggaa
     2521 atgcgtttgg tgcgtatgcc acagcctat
//
LOCUS       DAOQJE010000555         2549 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_29371_length_2549_cov_2.581796, whole genome shotgun sequence.
ACCESSION   DAOQJE010000555 DAOQJE010000000
VERSION     DAOQJE010000555.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2549)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2549)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2549
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29371_length_2549_cov_2.581796"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(156..1514)
                     /locus_tag="PLT08_13300"
     CDS             complement(156..1514)
                     /locus_tag="PLT08_13300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE05495.1"
                     /translation="MPIKNNIPTVIVSNFAEAHVDFLGRCADPKEREELILSEAYHAD
                     RTLLWAGDPKLVVVSYPIAHASLIRDLGFTQTDYTYPHAPTFYLCKDILREEDLIQKI
                     VNFAGQDQKLNLIPYATTPEFYELVSFLRVSKGLRVETPESPEVKNFWLRDYIDTKNG
                     FRSLASMWLNDAEGFLTLGVSCYNMDHAIRVIEWFSQNGESCVVKADTGESGIGTVVI
                     HPYGDQEKKEVFNRLKNDPYFADELIVVERFVPSKNQISPSLEVKVPSLGEGKPEVTY
                     VSKQLFLEFGDFCGIQVDKSIYQSSWYPKLESSGLLLAERLQEMGYVGHFDIDCVVSD
                     DDHLYLLEINARRTGGTHVHEFAKHIYGDDYIEKISCVSYEAMDSGSITNPKELQDAL
                     ADYLLPINGDERYGLVITITRALHNHRFGCIAVAPTADLALKLQTEALLHLQNFNAQI
                     DT"
     gene            complement(1529..>2549)
                     /locus_tag="PLT08_13305"
     CDS             complement(1529..>2549)
                     /locus_tag="PLT08_13305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE05496.1"
                     /translation="TVVFASTYYWFYDFAQKEAMNQIKEGLYDTIIGATRTGILDKGD
                     TVQIIDGDQMEGLVKEGKVRADGLTDDTRYWDQVQVLCEIRRVEPRASPYTYIAGEEP
                     GEIIFIASWGACLPDPDWNNFVKFRVPYNTNTGPNLAGLEQVVFQTDNGWCTYDDPTC
                     VPEIYGDKFGSWVSAYAPITNSKGETVGALGIDFTSTYVNQVQEQILRSIYVAFSITY
                     IILVVLVYFVAQFLTRPMVGLTRAAEQIGEGNYETGLKYLGELGLSDKYPDEIEKMQG
                     VFKGMIDKVYKREEVLRQQVRDLKIEIDQSKRNKQVNEIVESEFFQDLRTKAQKMRDQ
                     QKSKS"
BASE COUNT          788 a          588 c          456 g          717 t
ORIGIN      
        1 tttttaaaag caagaaatac tactaaatta gccccaaaaa caacaaggcg taaagtatat
       61 acaattatac cgtcaaaaat aatgacaaaa atattaacaa ccgttctaac caccaaacat
      121 atctacatat actatataga tgaaaaaatt cctgattaag tgtctatttg ggcattaaag
      181 ttttgaagat gcaataaagc ctctgtttgc aatttcaatg ccaaatcggc tgttggggca
      241 acagcgatac agccaaagcg gtggttgtga agcgcacgag taatggtaat cactaatcca
      301 tatcgctcat ccccattaat gggcaatagg taatcagcaa gcgcatcctg aagttcctta
      361 ggattcgtaa tactgccgga atccatcgct tcgtaactta cacaagatat cttttcaatg
      421 taatcgtcgc cataaatgtg cttcgcaaat tcgtggacat gcgtaccacc agtgcgacgc
      481 gcattaatct ctaaaagata caaatggtcg tcatcactga caacacaatc aatgtcaaaa
      541 tgtccaacat atcccatttc ctgtaagcgt tctgcaagca acagtccact agactcaagc
      601 tttggatacc atgaagattg atatatactc ttatcgactt ggataccgca aaaatctcca
      661 aactctaaaa agagttgctt tgaaacataa gtaacttccg gcttgccttc acctaaagaa
      721 ggaactttta cttctaacga aggggatatt tgattttttg aagggacaaa tctttcaacg
      781 acaatcagtt catccgcaaa gtatggatcg ttcttgagtc gattaaatac ctctttcttt
      841 tcttgatcgc cgtaaggatg aatgacaacg gttccaatcc cgctttcacc tgtatccgcc
      901 tttacaacac aagactctcc gttttggcta aaccactcta taacccgaat ggcatgatcc
      961 atgttatagc atgaaacacc caatgtaaga aaaccttctg catcgttaag ccacatagaa
     1021 gctaatgaac gaaaaccatt cttagtatca atataatcgc gcaaccaaaa atttttcact
     1081 tcagggcttt caggagtttc tacgcgcagc cctttggaaa cacgcagaaa actaactaat
     1141 tcataaaatt caggcgttgt agcatatgga atgagattaa gtttctggtc ttgccctgca
     1201 aagttaacaa tcttttggat taaatcttct tcacgaagaa tgtctttaca taaataaaac
     1261 gtgggggcgt gtggatacgt ataatcagtt tgtgtaaagc ctagatcacg gattagggaa
     1321 gcatgggcaa ttgggtagct aacaacaacc aacttagggt cacccgccca cagtagcgtt
     1381 cggtccgcat gataagcttc gctaagaatc aattcttcgc gttcttttgg gtcagcacaa
     1441 cgccctaaaa agtcaacgtg agcttcagcg aaattgctca cgatgactgt aggaatatta
     1501 ttcttaattg gcatcaactt attccgggct aacttttaga cttttgctgg tcacgcatct
     1561 tctgagcttt tgtccgtaaa tcctggaaaa actctgactc tacaatctcg ttgacttgtt
     1621 tattcctctt tgattgatca atctcaatct tcaggtcacg aacttgttga cgtaaaacct
     1681 cttcacgttt gtatacctta tcgatcatgc ctttgaaaac cccttgcatt ttctcaattt
     1741 catctggata tttatctgaa agccccaact cgccaaggta tttcaaacca gtttcataat
     1801 ttccttcacc tatttgttca gcagcgcggg tgagaccaac cattgggcgt gttaagaact
     1861 gcgcaacaaa atagactaga acaacgagaa taatatatgt aatagagaag gcgacatata
     1921 tactgcgtaa gatctgctct tgcacttgat tgacatatgt agatgtaaag tcaataccca
     1981 atgcaccgac cgtttcgccc tttgaattcg taattggggc atacgcagaa acccaagaac
     2041 caaacttatc accatagatc tcgggcacac aagtgggatc gtcatatgta caccaaccat
     2101 tatctgtttg gaaaacgacc tgctccagac ccgcaaggtt tggacctgtg ttcgtattat
     2161 atgggacacg gaatttcaca aagttattcc aatctgggtc aggcaaacaa gcaccccaac
     2221 tggcaataaa gataatttca ccaggctctt ccccagcaat gtaggtgtaa ggactagcac
     2281 gaggttctac acgtctgatc tcgcagagaa cttgaacctg atcccaatag cgagtgtcat
     2341 ctgtcaaacc atctgcacgg actttccctt ccttcactag cccttccatc tgatcaccat
     2401 caataatttg aacagtatcc cctttatcca aaatgcctgt acgtgtagcc ccaataatcg
     2461 tgtcgtacaa tccctctttg atctgattca ttgcctcttt ttgggcaaag tcgtagaacc
     2521 aataataggt agaagcaaat actactgta
//
LOCUS       DAOQJE010000556         2545 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_29436_length_2545_cov_3.151004, whole genome shotgun sequence.
ACCESSION   DAOQJE010000556 DAOQJE010000000
VERSION     DAOQJE010000556.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2545)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2545)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2545
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29436_length_2545_cov_3.151004"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1247
                     /locus_tag="PLT08_13310"
     CDS             <1..1247
                     /locus_tag="PLT08_13310"
                     /inference="COORDINATES: protein motif:HMM:NF013704.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006306 - DNA methylation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DNA methyltransferase"
                     /protein_id="HNE05497.1"
                     /translation="YSVHGLHEYKGKFNPQVVRGVLNLLGVSDKANVLDPFSGSGTTL
                     VECAHAKIKAVGWDINPLAVYIANAKLKAMSTPANDLQRIFTNLINKFKDDEVKIKLP
                     IPSDDERAEYLWSWFDKKQISEIERLRTLILKYGKDNEEIFLALASDLLRDYSLQEPT
                     DLRIRRRFSPYPDETFFEAYQKKTALFINNLIATQNLLDVLPHKNHAYLLDSRKLENV
                     SKSHKPKGGFQAAITSPPYATALPYIDTQRLSLVWLGLISPKEISNLEAKLTGSREFA
                     QEQKKLWSQALQDNTDNLPKDLHQYCMTLHQSLSSKDGFRRQSTPMLLYRYLSDMKKT
                     FETVIDVMATSAPFALIVGHNRTILSGKEFDIDTPSLLKTIAEEVGWKHKESTLLQTY
                     QRYGLHSSNAVKAETLLIVEKP"
     gene            1244..2332
                     /locus_tag="PLT08_13315"
     CDS             1244..2332
                     /locus_tag="PLT08_13315"
                     /inference="COORDINATES: protein motif:HMM:NF037074.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE05498.1"
                     /translation="MIEFGLHPNIYATGNHKGLRSMLEDVWIRSHKPGNGTLYILAGF
                     ANYNGGARFYKTFKEHTEKGGKIVAFLGGSSSQRTTSRQVVEALIECGADVNITNRKS
                     LMHAKCYGIKTDEGEKLIVSSGNFTGPGMAQNVEAALLLGTDATAQMGFSWDELSNGM
                     LRQNWITHKPKVLNPQQPAWGLLYDETPGAITIDQSEKSTMIIKLGHADTARIMARPG
                     TLASKGTQYFWLSKDCFDFFPALTIRNNRGYKGTLSTLITMHYIDLGTTDNNCRVTFE
                     AENNLDFRLGTGFLRNKRLAKKDDLACISRVGEDEYELRIIQKDSRNYNSLLSYAISF
                     IGHQGKQYGFIENEQFEDITNARLGELG"
BASE COUNT          866 a          496 c          514 g          669 t
ORIGIN      
        1 ggtattcagt acatggactt cacgaataca aaggtaaatt taacccgcaa gtcgttaggg
       61 gcgtactaaa cctacttggc gtatccgaca aagccaatgt acttgacccc ttctctggta
      121 gtggaactac tttagtagag tgcgctcacg caaaaataaa agccgttgga tgggacataa
      181 atcctttagc cgtctatatc gcaaatgcta aattaaaagc gatgtccaca cctgccaatg
      241 acttacagcg aatctttaca aacttaatta ataaattcaa agacgatgaa gtaaaaataa
      301 aacttccaat tcctagcgat gatgaaagag ccgaatacct ttggagttgg ttcgacaaaa
      361 aacaaatttc cgaaattgag cgattaagaa cgcttatctt aaaatacggc aaggataatg
      421 aagagatatt tttggcattg gctagtgact tgcttcggga ttattctctc caagagccaa
      481 cagacttaag aattcgccgt aggtttagcc cataccctga tgaaactttt tttgaagcat
      541 accaaaagaa gactgcttta tttatcaaca acttgattgc tactcagaat ttgctagatg
      601 ttttgccgca caaaaatcac gcttacttac ttgacagcag aaaattagaa aatgtgtcca
      661 agtctcacaa gccaaaaggc ggttttcaag cggcgattac tagcccaccg tatgccaccg
      721 ccctacccta tattgatacc caacgattaa gtttagtttg gttaggttta atctccccaa
      781 aagagataag caacctagaa gcaaaattaa ctggtagccg tgagtttgca caagagcaga
      841 aaaaactctg gagtcaggca ctgcaagata atacagataa tttgccaaaa gacctacatc
      901 aatactgtat gacattgcac caaagcctat catcgaaaga cggtttcaga aggcagtcaa
      961 cgcccatgtt gctttacagg tatcttagtg atatgaagaa aacgtttgaa acagtaattg
     1021 atgtaatggc gacttcagcc ccctttgccc tgattgtcgg gcataataga acaatattaa
     1081 gtggcaaaga gtttgacatt gatactccat cattgttgaa aacaatagca gaagaagttg
     1141 gatggaagca taaagaaagc actctcttac aaacatatca aaggtatggt ttgcattcat
     1201 ccaatgctgt caaagcagag acattattaa ttgtggagaa accatgattg aatttggatt
     1261 acaccccaac atctacgcaa ctggtaatca caaaggtctt cggagtatgt tagaagatgt
     1321 ttggatacgc tcgcataaac ctggaaatgg gactttatat attcttgctg gttttgcaaa
     1381 ttacaatggc ggagcaagat tttataaaac tttcaaagaa cacaccgaaa aaggcgggaa
     1441 aatcgtagcc tttcttggtg gcagttcatc gcaacgaact acaagccgtc aagtagttga
     1501 agcattgatt gagtgtggag cagacgttaa catcacaaat cgtaaaagcc taatgcacgc
     1561 taaatgctat ggcataaaaa cagatgaagg tgaaaaactt attgtctcgt ctggaaattt
     1621 cacgggacct ggaatggctc aaaatgtaga agcggcttta ttgttaggca cagacgctac
     1681 agcgcagatg ggattttctt gggacgagtt atcaaatgga atgctaagac aaaactggat
     1741 aactcataaa cccaaagttt tgaatccaca acagccagca tggggattgt tgtatgacga
     1801 aactccaggg gcaattacta ttgaccagag tgaaaagtca acaatgatta taaaattagg
     1861 acacgcagat acagcacgaa ttatggcacg tccaggaact ctcgcgtcaa aaggaactca
     1921 atatttctgg ctcagtaaag attgtttcga tttctttcct gcacttacta taagaaataa
     1981 caggggttac aaggggactc tttcgacctt aataaccatg cactatatag acctcggaac
     2041 aactgacaat aattgccgag taacttttga agccgaaaat aatcttgatt ttcgtttagg
     2101 cacaggtttt ctaagaaata aaagattagc aaaaaaagat gatttggctt gtatttcgag
     2161 agtcggggaa gatgaatatg aattaagaat tattcaaaag gactcaagaa actacaacag
     2221 cctcttatct tatgcaataa gttttatcgg acatcaaggt aaacaatacg ggttcattga
     2281 aaatgagcaa tttgaagata ttacaaatgc acgtcttggt gaattaggtt aataaagttg
     2341 agtctcttaa aaatcaaaaa ggtcttccaa gtcaaaagcg tgccaacaaa gcgtgcacct
     2401 gacgtgtggg attctgcggc attttcgagc atttttctag cctcagcatt ttcctgctcc
     2461 caaacagaat ccacgcccgc ccacacgcag gtaacgcaaa ccgttggcaa gaacttgcag
     2521 taaatcttta ggttagcttt tcaaa
//
LOCUS       DAOQJE010000557         2539 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_29543_length_2539_cov_8.201691, whole genome shotgun sequence.
ACCESSION   DAOQJE010000557 DAOQJE010000000
VERSION     DAOQJE010000557.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2539)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2539)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2539
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29543_length_2539_cov_8.201691"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..568)
                     /locus_tag="PLT08_13320"
     CDS             complement(<1..568)
                     /locus_tag="PLT08_13320"
                     /inference="COORDINATES: protein motif:HMM:NF014075.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF115 domain-containing protein"
                     /protein_id="HNE05499.1"
                     /translation="MKDTFKQIIPQPLWQFARDTYDGFRRIPQLPEAYFHPWRRESIK
                     RLAALKDIHKGKRAFIIGNGPSLKQTDLSKLKNEITFGMNRFYLAFPDLGFTTSYICV
                     TNDLVVEQFVNDFLSLQVPQFIAWRSHRHYPAGLPTSNLPTFIYTTYTGPRFTTDVRA
                     RVWEGATVTNLALQMAYHMGIEKAILIGV"
     gene            complement(565..945)
                     /locus_tag="PLT08_13325"
     CDS             complement(565..945)
                     /locus_tag="PLT08_13325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE05500.1"
                     /translation="MTRARKKLSNRFQSSLFLAKLLFYFNAAWWLLLGIIFVYQMVED
                     KNSWSSVMVSIFFVVMIASLIVGARIVSEREKWAFVLCIVIAVANILFSIFGFLDFLF
                     VIAALFDLAILGSLYSLKGYYLTS"
     gene            complement(969..1319)
                     /locus_tag="PLT08_13330"
     CDS             complement(969..1319)
                     /locus_tag="PLT08_13330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE05501.1"
                     /translation="MKSLQPARYLLLLISAFLSVLSILTLTRLGERPGMVGWYAIYSI
                     FMLIDSVVVLVCYFRLKQQGQRIFQVSMLILLLNMVLTIFDQIGVVDVIFIVLNLFAL
                     ILLYMARKEIAPVK"
     gene            complement(1322..2077)
                     /locus_tag="PLT08_13335"
     CDS             complement(1322..2077)
                     /locus_tag="PLT08_13335"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HNE05502.1"
                     /translation="MTRTVLITGAGGGIGRACINHFSSHGWRVIGVDRSDFGDNFPKD
                     GRFIKADISQPDSVEQIFQQAKEFHPALDALVNNAAVQVAKPLVDTTVEEWDSVMASN
                     LRSVFLFVKLAHPLLKAGNGGSIVNVSSVHAVQTSVNIAAYAASKGGLLALTRAMAIE
                     FAPDNIRVNAILPGAVDTQMLRAGLGRGHVGAGDLQDRLDNLARKTVNGRVGKPEEIA
                     HAIYFLADNDQSSFMTGQSIIVDGGATARLSTE"
     gene            complement(2196..>2539)
                     /locus_tag="PLT08_13340"
     CDS             complement(2196..>2539)
                     /locus_tag="PLT08_13340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE05503.1"
                     /translation="PEMGVNFMSGRASPVKYVYYPLFLEGSLTKDIEDSYFADITSNP
                     PELILDCSHSVDAIPSLDVATREEQYSTPGVKKKMYIQPNMNDIFEFVEQNYTLENTI
                     ETCLVFRLKKD"
BASE COUNT          783 a          558 c          557 g          641 t
ORIGIN      
        1 cgacaccgat caagatcgct ttttcaatgc ccatatgata tgccatttgc aaagctaggt
       61 tggtaactgt ggcgccttcc caaacccgcg cacggacatc agttgtaaag cgcggaccgg
      121 tataggtggt gtagataaag gttggcaaat tagaggttgg caaacccgca gggtaatgac
      181 gatgagagcg ccaagcaatg aactgcggca cttgcaaaga aagaaagtca ttgacgaatt
      241 gttcaacgac aaggtcgtta gtaacgcaaa tgtatgaagt tgtaaaacca aggtcaggga
      301 aagcaagata aaaacgattc ataccaaagg taatttcgtt cttgagcttg ctaaggtcag
      361 tttgctttag agagggtcca tttccgataa tgaaggcacg tttgccttta tgaatatctt
      421 tgagtgctgc aagacgctta atactctcac gccgccatgg atgaaaatac gcttcaggga
      481 gttgggggat gcgacgaaag ccatcatagg tgtcgcgcgc aaattgccag agaggctgag
      541 gaatgatctg cttaaatgta tccttcatga ggttaggtaa tagcctttga gagaatataa
      601 actaccgaga atggcgaggt caaataaagc ggcgatcacg aaaagaaaat ccaggaagcc
      661 aaaaatggaa aaaaggatat ttgccacagc aatgacgata caaagcacaa atgcccattt
      721 ttcacgttcg ctcacaatgc gagcgcccac tataagagaa gcgatcatga ccacaaaaaa
      781 gatggacacc atcaccgatg accaactatt cttatcttca accatttggt atacaaagat
      841 tatccctaat aaaagccacc aagcggcatt gaaatagaac aagagctttg ccaaaaagag
      901 gctggactga aatcgattgc tcagcttttt tcttgcgcgt gtcattttct acagaccttt
      961 tcttgtattt attttacagg tgcaatttct ttacgtgcca tatagagcaa gatcaatgcg
     1021 aataaattca ggacaataaa aataacatca acaaccccga tttgatcaaa tattgtcaaa
     1081 accatattca aaagcagaat gagcatactc acttgaaaga tgcgttgacc ttgctgcttc
     1141 aaccgaaaat agcagaccaa cacaactact gaatctatga gcataaaaat tgaatatatt
     1201 gcataccaac ccaccattcc tggtctttcg ccaagacgag ttaaggtcag aatactcagt
     1261 acagacaaga aggcagatat cagtaagagc aaataacgcg caggttgcag gcttttcatt
     1321 tctactccgt gcttagacgt gccgttgcgc caccatctac gattatggat tgacctgtca
     1381 tgaatgaaga ttggtcatta tcagcaagga agtaaatagc atgagcgatt tcttctggct
     1441 tgcccacacg cccattgacc gtcttgcggg caagattatc caaccggtct tgcaggtcgc
     1501 ctgcacctac atgtccacgc ccaaggccgg ctctcagcat ttgagtatct acagcgccgg
     1561 gcaaaatagc attgacgcga atattatcgg gcgcgaattc aattgccatg gcgcgtgtaa
     1621 gggcgagtaa accaccttta gaggcggcat atgcggcaat attgacggag gtctgcactg
     1681 catggacgga agaaacattc acaatggagc cgccattccc tgctttgagc aaagggtgag
     1741 cgagcttaac gaacaaaaat actgaacgca aattcgatgc catcaccgaa tcccattctt
     1801 ctacggtggt atctactaat ggcttggcta cttgcacagc agcgttattg accaatgcat
     1861 ccaaagcagg gtgaaattct tttgcttgtt gaaaaatctg ttcaacagag tctggttgtg
     1921 aaatatctgc tttgataaaa cgcccatctt ttggaaaatt atctccaaag tcagagcgat
     1981 ctacgccaat aacccgccaa ccgtggcttg aaaaatgatt aatgcaggcg cgtccaattc
     2041 cgccgcctgc gccagtgatg agaactgttc tagtcatagg gtaattttac gtcgaaatct
     2101 agctttgtgc ctgaagcaaa atgaataaaa gcaacagacg cgaatttccc aattcgcgtc
     2161 tgttgctttt actgatctat agtaatttta caaacctagt cttttttaag cctgaaaact
     2221 aggcaagtct ctatcgtatt ttcaagtgtg taattctgct caacgaactc aaagatatcg
     2281 ttcatattag gttgaatata cattttcttc ttcacacccg gtgtagagta ttgttcctcg
     2341 cgtgttgcaa catccaaaga gggaatggca tccacagagt gggaacaatc aagaatcagt
     2401 tcaggtgggt tgcttgtgat gtctgcgaag taactatctt caatatcttt ggtgagagac
     2461 ccttccaaaa agagcggata atacacatac ttcaccggcg aagcccgacc tgacatgaag
     2521 ttcacgccca tttcgggat
//
LOCUS       DAOQJE010000558         2539 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_29545_length_2539_cov_7.604670, whole genome shotgun sequence.
ACCESSION   DAOQJE010000558 DAOQJE010000000
VERSION     DAOQJE010000558.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2539)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2539)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2539
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29545_length_2539_cov_7.604670"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..473
                     /locus_tag="PLT08_13345"
     CDS             <1..473
                     /locus_tag="PLT08_13345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HNE05504.1"
                     /translation="IVILTARGEEQDRVRGLDLGADDYLIKPFSATELLARVRAVLRR
                     AHNPTDAGQVRFFTHDDLKIDFARAEVWRGEEAVSLSATEYRLLLQFAHNVGKILTSE
                     ELLTSVWGVEYKNDKEILWVSIARLRQKLEKDAHTPRHIVTRSGLGYLMPPIET"
     gene            512..1660
                     /locus_tag="PLT08_13350"
     CDS             512..1660
                     /locus_tag="PLT08_13350"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNE05505.1"
                     /translation="MSQKKILIVDSDVASRNFIARKLQELDYETLQAGSGKEGLIYTW
                     RDHPDLVIADPALTDIKGEEFATKLRNDPRTANLPIVALSSDHNPARIKSCQDAGFND
                     YITKSGQAVPMLKDIINRLFGVTQEDMKQGGLMIVFLSAKGGTGTSSVCANTAVNITK
                     DQPEARIAVVDLVFPIGSIAQIVGYEGTDNIVTVADRNPLETDVDFLKTLPRIEPWNF
                     NLLAGSPDPELSNQLNVGRIWDIITGLKSSYDYVLIDIGRSLSKITLPLIQHADLISL
                     LISTDVSTISLTKTILDFLKSKGVKPEAIYTILNRHASLEGLSKREAETILEKNVNTA
                     IPYLGTNFGFANSQHRPFTLKFPNDTASIIFHDSAREMANLAKKIRSQ"
     gene            1693..1986
                     /locus_tag="PLT08_13355"
     CDS             1693..1986
                     /locus_tag="PLT08_13355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE05506.1"
                     /translation="MSIEYDDKGKYYTNVIQKVPVPAMVQTTTNLIRGIVHVRHGERL
                     KDEIEGSEQFVAMTDVSVYSADGKELYTGPFLALQKGQIVWVMPLNETQKGEE"
     gene            1983..>2539
                     /locus_tag="PLT08_13360"
     CDS             1983..>2539
                     /locus_tag="PLT08_13360"
                     /inference="COORDINATES: protein motif:HMM:NF012652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HNE05507.1"
                     /translation="MNTPLSGAPQRLTGQDLIRLKKYLADNLLRALETEGIEASQRVT
                     YIQQNIGRVFDTTQLKFPEDLKREIFKQVLNDLLGFGPIQPLLDDPDISEIMVNGPKK
                     IFIEKKGQLTKSEITFDDDDHVLRVIDRIIMPLGRRVDFDSPTVDARLPDGSRVNAVV
                     RPVSIDGPSITIRKFRKDKLKAEDL"
BASE COUNT          723 a          554 c          588 g          674 t
ORIGIN      
        1 ctattgttat tctgacggca cgcggcgaag aacaagatcg tgttcgtggg ctagaccttg
       61 gagcagatga ttacctgatc aagcctttct ctgcaactga gttgcttgct cgtgttcgtg
      121 ctgtgctacg ccgtgctcat aacccaaccg atgcagggca agtgcgcttc tttacacatg
      181 atgacctgaa gatagacttt gcccgtgccg aagtgtggcg tggtgaagag gcggtttctc
      241 tctctgccac tgaatataga ctactgcttc aattcgctca taacgttggc aagattctca
      301 cttctgaaga attgctgacc agtgtttggg gtgttgaata caaaaatgat aaggaaattt
      361 tatgggtgag cattgcccgt ttacgccaga aacttgaaaa agatgctcac acccccaggc
      421 acattgttac ccgttctggg ctagggtatc tgatgccccc tattgaaacg taatacttgc
      481 gcataaaacg ggttgcgctc aggaacaaga tatgtctcaa aagaaaattt tgatcgtaga
      541 ctcagatgtc gcaagccgaa actttattgc acgaaaatta caagaacttg attatgaaac
      601 cttacaggca gggtctggta aggaaggtct gatttataca tggcgcgacc acccagacct
      661 tgtgattgct gaccctgctt tgaccgatat caagggggaa gagtttgcta ctaaactacg
      721 caacgacccg cggactgcta atttgcctat tgtcgcttta agtagtgacc ataaccctgc
      781 tcgtatcaaa tcttgccagg atgcgggctt caacgattac attacgaaat cgggacaagc
      841 ggtaccgatg ttgaaagaca tcatcaatcg cttgtttggc gttacacaag aagatatgaa
      901 acagggtggt ttgatgatcg tttttctcag tgccaaaggt ggcacaggta cttcttcagt
      961 ttgtgccaat acagcggtca atattacgaa ggatcaaccc gaagcacgaa ttgccgtggt
     1021 ggatttggta tttcctatcg ggtccattgc tcagattgta ggctacgaag ggactgataa
     1081 tattgtcact gttgcagacc gaaacccatt ggaaactgat gtggattttc ttaagacttt
     1141 gcccagaatc gagccttgga atttcaacct cttggcaggt tctccagacc ctgaactgag
     1201 taaccaactg aatgtggggc gcatttggga tattattact gggctgaaat cttcctatga
     1261 ttatgtccta attgacattg ggcgctctct ctcaaagatt acactgcctt taattcaaca
     1321 tgctgacttg atttctttgt taattagcac agatgtcagc accatctcgt tgaccaaaac
     1381 catattggat tttctcaaat cgaaaggggt aaagccagaa gcaatctata ccatcttaaa
     1441 tcgccatgct agtttggaag gtctaagcaa acgagaagct gaaacgattt tggagaaaaa
     1501 tgtaaatact gcaattccct acctcggtac aaatttcgga tttgcaaaca gccaacaccg
     1561 cccttttaca ttaaaattcc ctaacgatac agcctctata atttttcatg attctgccag
     1621 ggaaatggca aacctcgcaa aaaagatccg tagccaatag gtgtctttcc cgggcagagg
     1681 gagataaagc ccatgtctat agaatatgac gataaaggaa agtactacac aaatgttatt
     1741 caaaaagtgc ctgtgccagc catggtacag accacaacaa atctcatccg tgggatagtt
     1801 catgtacggc acggtgaacg tttgaaggat gagatagaag gcagcgagca atttgttgcc
     1861 atgacggatg tcagtgttta tagcgcggat gggaaagagc tgtacacagg tccattcttg
     1921 gctctacaaa aagggcagat cgtttgggtc atgccgttga atgagacaca aaaaggggaa
     1981 gaatgaacac accactttca ggcgctccac aacggttaac tgggcaggac cttattcgcc
     2041 tgaagaagta tctggcagat aatcttttac gtgcactcga aacagaaggg atcgaagcat
     2101 cgcagcgagt gacatatatt cagcaaaaca tcgggcgtgt atttgatact acacagctca
     2161 aattccctga agacctaaaa cgagaaattt ttaagcaggt tttgaatgac ctgttgggct
     2221 ttggtcccat ccaaccactt ttggacgacc cagatatttc tgaaatcatg gtgaatggac
     2281 ctaaaaagat tttcatcgag aaaaaggggc agctcaccaa gagcgagatc acctttgatg
     2341 atgacgatca tgtattgcgc gttattgacc gaatcattat gccattgggt cgccgcgtgg
     2401 attttgactc acctacggtc gatgcacgtt tacctgatgg ttcccgcgtc aatgctgtag
     2461 ttcgacccgt ctccattgat ggaccttcaa tcacaatccg taagtttcgc aaagataaac
     2521 tgaaggctga ggacttaat
//
LOCUS       DAOQJE010000559         2538 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_29564_length_2538_cov_5.776077, whole genome shotgun sequence.
ACCESSION   DAOQJE010000559 DAOQJE010000000
VERSION     DAOQJE010000559.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2538)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2538)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2538
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29564_length_2538_cov_5.776077"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1957
                     /locus_tag="PLT08_13365"
     CDS             <1..1957
                     /locus_tag="PLT08_13365"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HNE05508.1"
                     /translation="KVAIKIMRDHVEEDPDSRARFAREARVVATLDHPNIIQIYDYDL
                     VDGRPCIVMELVEGTSLAGYLKELHKRGEKLPHSVVAKLMKSIASAMDHAHERNIVHR
                     DIKPANILLRSKQGVIDLQAPLPEDVEPVLTDFGLVRLLDSNTKTSTGTVSGTPSYMS
                     PEQARGDSVDHRTDIYSLGVVLYEMLAGEVPFDAESSFGVLMKHLNEPPPPINGISAG
                     MQAIINRALAKDPKDRFASAIEMVNEFADVLEGKSVSKETLRINSLPKETTANAKQAT
                     SSFKTPLLVGGGIIALALIVSLGVFIFRQPRETNVPSQNNNQLPNPSQPIGSVVFDDY
                     NELVDNATVTISGIPLPPAGQHFEVWFLGQGGEIKLNAGPVIFTGQNGQLIFSDSNGA
                     NLLSQFDEMMVTVEPDNDPNPDQPSQEVAASSVFPPQAFVHIRHILVSIGMTPNGTAL
                     IQGVWDTADTIDTSVLELRDAFNEGDEATLRIKNEEIINQLVGEENLQLYKDWNGDGE
                     VNDPNDGFGLLANGIEQGYIPLTISHTEFAIDAPDATTGIIQSGQNLIVCLQNMKGWS
                     EQLLEVALKLQSMPYGTGMESTIAEIETYSSLLLYGEDTNGNNRIEPLPGEAGADTAY
                     FAAYSIAQMPIFPGGRRMPQPANTTQP"
     gene            complement(2008..2319)
                     /locus_tag="PLT08_13370"
     CDS             complement(2008..2319)
                     /locus_tag="PLT08_13370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE05509.1"
                     /translation="MESQPFNSSPAPAPASGGNERVMAIASLVIGVINLCAWFLPICG
                     APLGIIGIVLGIFGMKDPSQKTLAIIGIALSAIGLLAACINGAVGAYMGVTGQGFNFS
                     P"
BASE COUNT          759 a          634 c          532 g          613 t
ORIGIN      
        1 caaagttgca attaaaatca tgcgcgatca tgtggaagaa gaccccgatt cgcgcgcacg
       61 ttttgctcgt gaagcacgtg ttgtagcaac cctcgatcac ccaaatatta tccaaatcta
      121 cgattatgat ttggtcgatg ggcgtccatg cattgtcatg gaacttgtcg aaggcacttc
      181 tctagctggc tatctaaaag aattgcacaa gcgcggcgaa aagcttccac actccgtagt
      241 tgccaaactc atgaaatcca tcgcctcagc catggaccat gcacatgaac gcaacattgt
      301 gcaccgcgat atcaaacctg ccaatattct gcttcggtcc aaacaaggtg tcatcgattt
      361 acaagctccc cttccagagg atgtagaacc ggttctaacc gactttggct tggtacgcct
      421 cctcgactcg aatacgaaga cctcaactgg taccgtctcc ggaacccctt cttacatgag
      481 ccctgaacaa gcccgtggag atagtgttga tcaccgaaca gatatctact ctctaggtgt
      541 tgttctttac gaaatgcttg ccggggaagt ccccttcgac gcggagtctt catttggtgt
      601 tctaatgaag cacctaaatg agccgccccc accgattaat ggtatttcag cgggtatgca
      661 agccattatc aatcgcgcac ttgcaaaaga tcccaaagat cgttttgctt cagcgattga
      721 aatggtcaat gaatttgccg atgtccttga aggtaaatct gtatcaaaag agacattaag
      781 aatcaatagt ttgccaaaag aaacaacggc aaatgcaaaa caagccactt catcgttcaa
      841 gactccccta ttggtgggcg gagggattat tgcccttgcc ctaatcgttt cactcggcgt
      901 gtttatcttc cgccagccgc gtgaaacaaa cgtaccatcc caaaataaca atcaattacc
      961 caatccaagt caacccattg gttctgttgt ctttgatgat tacaatgaat tggtcgataa
     1021 tgccacagtt accatcagcg gaatccccct accgccagcc gggcaacatt ttgaagtctg
     1081 gttcctgggt caaggcggag aaataaaact aaatgcgggt ccggtgatat ttaccgggca
     1141 aaatggacaa ttaattttct cagactcaaa cggcgcgaat cttctcagtc agtttgatga
     1201 gatgatggtc acggtagagc cggataatga tcccaacccc gaccaaccca gccaggaagt
     1261 tgccgcctct tcggtgttcc ctccgcaggc atttgtccat ataaggcata ttcttgtctc
     1321 cataggcatg acgccaaatg gaacagccct catccaaggc gtatgggaca cagccgatac
     1381 aatcgataca tctgttttag agcttagaga tgcattcaat gaaggcgatg aagcaactct
     1441 gcgaataaag aatgaagaaa ttattaatca acttgtagga gaagaaaacc ttcagttgta
     1501 taaagactgg aatggagatg gtgaggtcaa cgaccccaat gatgggtttg ggctacttgc
     1561 aaatggcata gaacaaggct acatcccctt gacgatcagc catacagaat ttgctataga
     1621 tgctcccgat gcgaccacag gcatcatcca aagcggacaa aacttaattg tttgtctgca
     1681 aaacatgaaa ggctggagcg aacaactcct tgaagtagcg cttaaacttc agagtatgcc
     1741 ctatggcacc ggtatggaat caaccattgc agaaatagaa acctattcaa gcctcttact
     1801 atatggcgaa gatacaaatg gtaacaatcg tattgaaccg ctccctggtg aagccggagc
     1861 cgataccgct tactttgccg catattctat tgcccaaatg cccatcttcc caggcggcag
     1921 gcgtatgcca caacccgcca atacaacaca gccataaaaa aaagctcttg cgatatgcaa
     1981 gagcttttta caacgcctga caaagaatta cggagagaaa ttaaaacctt gccccgtaac
     2041 gcccatataa gcacctacag caccattgat acaagccgca agcaaaccaa ttgcgcttaa
     2101 ggcaatacca atgatcgcca aggtcttctg tgaaggatcc ttcataccga agataccaag
     2161 cacaatacca attatgccaa gtggtgcgcc acagataggc aaaaaccatg cgcacaaatt
     2221 gatgacgcca atcacgagtg aagcgattgc catcacacgt tcattcccac cagaagcagg
     2281 ggcgggagca ggggaagagt tgaagggctg ggattccatt agacattctc ctttttgatt
     2341 tctaaaattt tacttcaaac tcggtatgaa gttatccatc cgaagtccta tctgtgattt
     2401 tcaatgcctc atccaaaaca tcaaagcctt cctgcaattc tttttcgcta ataatcaacg
     2461 gcgggatgat caacaccgta tgccaatgag tatatagaaa taaaccatga tcagtacaat
     2521 attttttcaa ggcagtca
//
LOCUS       DAOQJE010000560         2538 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_29566_length_2538_cov_5.329843, whole genome shotgun sequence.
ACCESSION   DAOQJE010000560 DAOQJE010000000
VERSION     DAOQJE010000560.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2538)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2538)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2538
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29566_length_2538_cov_5.329843"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..726)
                     /locus_tag="PLT08_13375"
     CDS             complement(<1..726)
                     /locus_tag="PLT08_13375"
                     /inference="COORDINATES: protein motif:HMM:NF012506.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046912 - acyltransferase activity, acyl
                     groups converted into alkyl on transfer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate/2-methylcitrate synthase"
                     /protein_id="HNE05510.1"
                     /translation="MILHEKMSAQLPAWRERIKSLAKEHGEVVVDKVTVGQIVGGMRD
                     IKSLLTDVSFVDPAEGIRFRGMSIPEVLKALPKAKGGKMPLVGGLFYLLLIGEVPTKE
                     QALEVEAEWAKRANVPDYVFKMLKTMPKETHPMTLFSQAVLALQNESVFAAKYHSMKK
                     DQYWEAALEDSLDLTAKLPIIAAYIYRMKFFGETSKPKYNPKQDYGLNFARMMKVSDK
                     KGYADLARLYFILHSDHESGNVSA"
     gene            complement(796..1731)
                     /gene="mdh"
                     /locus_tag="PLT08_13380"
     CDS             complement(796..1731)
                     /gene="mdh"
                     /locus_tag="PLT08_13380"
                     /EC_number="1.1.1.37"
                     /inference="COORDINATES: protein motif:HMM:NF004863.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HNE05511.1"
                     /translation="MKKISIIGAGNTGATAAHWIAERELADVVLLDVVEGMPQGKSLD
                     LLEAMPIIGKDSHVLGTNNYEDTKNSDIIIITAGIARKPGMSREDLLKTNAEIVGKAA
                     TETLKHSPNAIFIVLTNPLDTMAYLVMKKTGLPRERVIGQAGILDSARMRAFVAMEAN
                     VSVENVQCYVLGGHGDEMVPLTRHSNIAGVPLKEYFPADKLEAIVNRTRKGGGEIVNL
                     LKTGSAYYAPSMACVQMAEAILKDKKLIVPCATLMQGEYGLNDMYFGVPVMLGAGGME
                     KVIEYKFDADEKAMFEKSAASVRESHDALKSVVSL"
     gene            complement(1964..>2538)
                     /locus_tag="PLT08_13385"
     CDS             complement(1964..>2538)
                     /locus_tag="PLT08_13385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HNE05512.1"
                     /translation="YEMTGRGVSYCATCDGWFFKDKKVVIVGGGDSAFEEALFITRYA
                     STVTLIHRREEFRAGAILQRRAKEHPKMNFILNTVVTEVNQGDKLTTLKLKNVQTNEE
                     STFDTDGLFIFIGHTPNTDMFKGKLEMNELGYLKINDKMETSIPGVFAAGEVADATFK
                     QVVTSAGMGAAAAIQATRYLESLEEEAAHS"
BASE COUNT          622 a          653 c          604 g          659 t
ORIGIN      
        1 agccgaaacg tttccagact cgtgatcact gtgcaagatg aagtacaagc gggcaaggtc
       61 ggcatagcct ttcttatccg aaaccttcat catgcgggcg aagttcaaac cataatcttg
      121 tttggggttg tatttcggct tgctggtttc gccgaagaat ttcatgcggt agatatacgc
      181 cgcaatgata ggaagcttcg ccgtgaggtc gagactatct tcgagagcgg cttcccaata
      241 ttgatctttc ttcatgctgt gatacttcgc cgcaaagacc gactcattct gcaaagcgag
      301 caccgcttgc gagaagagcg tcatggggtg agtttctttc ggcatggtct tcaacatctt
      361 gaagacataa tcaggcacat tcgcgcgctt cgcccattct gcttccactt caagggcttg
      421 ttccttggta ggcacttcgc cgatcaacag aaggtagaac aagccaccca ctaaaggcat
      481 cttgccgccc tttgccttgg gcagcgcctt tagcacttct ggaatagaca taccgcggaa
      541 gcgaatgcct tcagccgggt ctacaaaaga gacatcggtg agcagggatt tgatatcgcg
      601 cataccgcca acgatctgac cgacggtaac tttatcaacc acaacttcgc cgtgttcttt
      661 agcaaggctc ttgatgcgtt cgcgccaggc aggcaattgg gcagacattt tttcgtgcaa
      721 aatcatggga ggctcctatt tttcaataca aaagtcgaaa gccatctttg accttcgact
      781 tttgaccttt gatagttata gactcacaac gctcttgagg gcgtcgtgtg attctcgaac
      841 agaagcagcc gatttctcga acattgcctt ttcgtcggca tcaaacttgt attcgatcac
      901 tttttccatg ccgcctgcac ccaacatgac gggcacaccg aagtacatat cgttcaaacc
      961 atattcaccc tgcatcaacg tagcgcaagg gacgatgagt ttcttatcct tcaaaatggc
     1021 ttctgccatc tgaacacaag ccatggaagg cgcataatag gcagaacccg tcttgagcaa
     1081 gttcacgatc tcgccgccac ccttgcgggt gcggttgacg atcgcttcga gcttatcagc
     1141 cgggaaatat tccttgagcg gcacacctgc aatattggag tgacgagtaa gcggaaccat
     1201 ttcgtcgccg tgcccaccca acacatagca ttgaacattt tcaacagaaa cattggcttc
     1261 catcgcaacg aaagcacgca tacgggcaga gtccaaaata cccgcctgcc caatgacacg
     1321 ttcgcgcggt aaaccagttt tcttcatgac caagtacgcc atcgtatcaa gcgggttggt
     1381 cagcacaata aaaatagcat tcggagaatg cttaagggtt tcagtagcag ccttgcccac
     1441 gatctcggca ttggtcttga gcaagtcttc gcggctcatg ccgggcttgc gagcaatacc
     1501 agcagtgata ataatgatgt ctgaattctt tgtgtcttcg taattgttgg ttcccaacac
     1561 atgcgagtct ttcccaataa tcggcatggc ttcgagcagg tccaggcttt ttccttgcgg
     1621 cattccctct acaacatcta gcaaaacgac atcggcgagt tcacgttcgg cgatccaatg
     1681 cgcagcggtg gcgccggtat tgcccgcgcc aataattgag attttcttca tcgtacttcc
     1741 tccaatggga tcggctaaaa agccgtattt ttttataatt ttatccgaaa gtgacctaga
     1801 ccaaaagtac aaaatgtcac atttttggac aaatccactc aagaaaacag actcgccgct
     1861 aaaagcgagt ctgttggtgg ataaaattta gaggatgacc ctgcaccatg gacggaatca
     1921 gctgacccgt agagtgaggc gtggctcgtc atccgcttct ttcttaactg tgggctgctt
     1981 cctcttccag cgactcgaga taacgggtcg cctgaatggc tgccgcagcc cccatgcccg
     2041 ccgaggtgac cacttgcttg aaggtggcat ctgccacctc ccctgctgcg aacacgccgg
     2101 gaatgctggt ttccattttg tcgttgatct tgagatagcc aagttcgttc atttcgagct
     2161 tgcctttgaa catatcggta ttcggtgtat gaccgataaa aatgaacaga ccgtctgtat
     2221 caaaggtgct ttcttcgttg gtttgaacat tcttgagttt gagggtagtg agcttgtcgc
     2281 cttggttcac ttcggtgact acggtattca agatgaagtt catcttgggg tgttctttgg
     2341 cacggcgttg caaaatagca ccggcgcgga attcttcacg gcggtgaatg agggtaacgg
     2401 ttgaggcata acgagtgatg aaaagtgctt cttcaaaggc gctatcgcca ccgcctacaa
     2461 tgacaacctt tttatctttg aagaaccagc catcacaagt ggcgcagtac gaaacaccgc
     2521 gaccggtcat ttcatatt
//
LOCUS       DAOQJE010000561         2537 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_29586_length_2537_cov_5.286060, whole genome shotgun sequence.
ACCESSION   DAOQJE010000561 DAOQJE010000000
VERSION     DAOQJE010000561.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2537)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2537)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2537
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29586_length_2537_cov_5.286060"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..936)
                     /locus_tag="PLT08_13390"
     CDS             complement(<1..936)
                     /locus_tag="PLT08_13390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006614 - SRP-dependent cotranslational
                     protein targeting to membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle receptor subunit
                     alpha"
                     /protein_id="HNE05513.1"
                     /translation="MFETLTGRLNQIFDNLRKRGKLSEADVDAAMKEVRLALLEADVH
                     FSVVKTFIAKVRERAVGAEVSKALNPGQQVIKIVNEELVATLGEPAPLNLTGPKPRVV
                     MMVGLQGAGKTTVTGKLARLLKSKGERILMVAGDPYRPAAVKQLQQLGERVGVDVEAD
                     PSIKPQELAKRALDKAEKGGYSLMLVDTAGRSQMDAQLMDELKAIHANVKPVDTLLVI
                     DSMIGQEALNIAQGFKETIDLTGLILTKMDGDSRGGAAISIRSVTGVPIKFIGTGEKL
                     DALETYDPSRLSSRILGMGDLIGLIEKAEAAYDAET"
     gene            1187..>2537
                     /locus_tag="PLT08_13395"
     CDS             1187..>2537
                     /locus_tag="PLT08_13395"
                     /inference="COORDINATES: protein motif:HMM:NF023128.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNE05514.1"
                     /translation="MATEAIVNDRKEIFGWAMYDWANSAFSTTVGTVFLGPYVASLAR
                     AAAEAAGTSTVSFLGIPVAPDSFLPYCISFSVGMQVLFLPILGAIADYSHLRKTMMRI
                     FATIGAIATILMFMLTGGLWWLGGVLFILANLTFGAAIVFYNAYLPDIASEEERDRVS
                     SYGWAMGYLGGGILLALNLAFYIFSDKIGVPGDLAVRINLASAGIWWLGFSFFTWNRL
                     RSRHASNSLPAGETYASIGFKQLRKTFSEVRHFPETMKFLLAYFLYNDGIQTVIAVSA
                     TFAAAPLIQGGLEQDQTTLTAVILMIQFMAFFGALFWGKLAGWIGAKQSVIVSLVIWA
                     GVVIYAYGGLKGDNVTVQFFILGVFIALVMGGSQAISRSLFAQMIPNGKEAEFFAFYE
                     ISERGTSWIGPLVFGLANQMFNNLRVGILSLIFFFIVGLVLLPFVNVNKAIADAKKYD
                     "
BASE COUNT          556 a          609 c          666 g          706 t
ORIGIN      
        1 ggtctcggca tcgtaggcgg cttcggcttt ttcgatcaag ccgatgaggt cacccatgcc
       61 caaaatacgt gaagacaaac gggatgggtc gtaggtttcc aaagcatcga gcttttcacc
      121 tgtaccaata aatttgatcg gcacaccggt caccgaacga atggagatgg cagcaccacc
      181 acgtgagtcg ccgtccattt tggtgaggat aagtcccgtc aggtcgatgg tttctttgaa
      241 accttgagca atgttcaaag cttcctgacc gatcatggaa tcgatgacga gcagagtatc
      301 caccggcttg acattggcat ggatggcttt caattcgtcc atcaactgcg catccatctg
      361 ggaccgaccg gctgtatcga ccaacatcag gctgtagccg cccttttcag ctttatcgag
      421 tgcgcgcttc gcgagttcct gtggcttgat ggatgggtct gcttccacat ccacgcccac
      481 acgttcgcca agttgctgca actgtttgac cgccgctggg cgatatgggt cgccagctac
      541 catcaaaata cgctcacctt ttgatttgag taaacgagcc agtttgcctg taactgtcgt
      601 tttaccagca ccttgcaaac cgaccatcat caccacacgt ggtttgggtc ccgtgaggtt
      661 tagcggagcg ggttcaccca gggtggcgac aagttcctcg ttgacgatct tgatgacttg
      721 ctgacccggg ttcaacgcct tcgaaacttc tgcacccacg gcacgttcgc ggactttggc
      781 aatgaaagtt ttgaccacgc taaaatgcac atccgcttcg agcaaggcga ggcgcacttc
      841 tttcatggcg gcatcgacat cggcttcgga gagctttccg cgtttgcgca aattgtcgaa
      901 gatttggttg agacgtccgg taagagtttc aaacataggg gtataaatga ctgccattaa
      961 gacaaaatgg caagattcct tttcagtgta agaagaggca ttttagcgcg gagcaggttg
     1021 agggtcaagc aggaggcgcg gttcttaatc cataaaaatt ggcaaaacca ttcacagaga
     1081 cgagggtttt tattctcaca atgttaaccg aacatctgtg ggttgtccgt atataatacg
     1141 ggcagacgtg aatccataac atcaatccag ttcgaggaga agaggcatgg caacggaagc
     1201 gatcgtcaat gaccggaaag agatttttgg gtgggcaatg tacgactggg caaactcggc
     1261 ttttagcacg actgttggaa ccgtattttt aggaccgtat gtggcaagtt tggcgcgcgc
     1321 tgcagcagaa gcggctggaa ccagcaccgt ttcgttctta ggaattcctg ttgcaccaga
     1381 ttcttttcta ccgtactgta tttcattttc tgtaggtatg caggttttgt tcctgccaat
     1441 ccttggtgca attgcagatt actcgcactt gcgcaagacc atgatgcgca tctttgcgac
     1501 catcggcgcg attgccacta ttttgatgtt catgctcaca ggcgggcttt ggtggttagg
     1561 cggtgtgctc tttattcttg ccaacctgac ctttggtgct gctattgttt tctataacgc
     1621 ctatttacct gatattgcca gcgaagaaga acgagaccgt gtctcttcgt atggttgggc
     1681 gatgggctat ttgggtggcg gcattttgct ggcgcttaac ctggcttttt atattttcag
     1741 tgacaagatc ggtgtgcctg gcgatttggc agtgcgcatc aaccttgcat cggcgggtat
     1801 ttggtggctt ggcttctcgt tctttacttg gaatcgtttg cgctcacgcc atgcgtcgaa
     1861 ctccttacct gccggtgaga cctatgccag cattggtttc aaacagttga gaaaaacttt
     1921 cagcgaagta cgtcacttcc ctgaaacgat gaaattcctt ttggcatact tcttgtacaa
     1981 cgacggaatt cagaccgtga tcgccgtttc tgcaaccttc gccgctgcgc ctctgattca
     2041 gggcgggctg gaacaagacc agacgactct caccgctgtg atcttgatga tccaattcat
     2101 ggcgttcttt ggagcattat tctggggcaa gttggctggc tggatcggtg ccaaacagtc
     2161 ggtgattgtc agccttgtga tctgggcagg cgtggtgatt tacgcctacg gtggattgaa
     2221 gggtgataac gtcacagttc agttcttcat tttgggcgtg ttcattgcct tggtcatggg
     2281 tggttcgcaa gccatcagcc gcagtttgtt cgcgcagatg atcccaaatg gcaaggaagc
     2341 cgaattcttc gccttctatg aaatcagcga acgcggcact tcatggatcg gaccgttggt
     2401 tttcggtctt gccaaccaga tgttcaataa cttgcgtgtg ggtattttgt ctttgatctt
     2461 cttctttatc gtaggcttgg ttttgctgcc gtttgtcaac gtcaacaaag ccattgcaga
     2521 tgcaaagaag tacgata
//
LOCUS       DAOQJE010000562         2536 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_29604_length_2536_cov_6.428053, whole genome shotgun sequence.
ACCESSION   DAOQJE010000562 DAOQJE010000000
VERSION     DAOQJE010000562.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2536)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2536)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2536
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29604_length_2536_cov_6.428053"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..769
                     /locus_tag="PLT08_13400"
     CDS             <1..769
                     /locus_tag="PLT08_13400"
                     /inference="COORDINATES: protein motif:HMM:NF015411.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="NAD(P)-binding domain-containing protein"
                     /protein_id="HNE05515.1"
                     /translation="VAEGATAAGSPKEVAEQVDIVFTNLPDSPDVEKVALDEVNGIIA
                     GAKPGTIVVDNSTIKPASARLIAAKLKEKGIFSLDAPVSGGDIGAKNGTLTIMVGGEA
                     EALEKVMPVFQAMGKSVTHVGEAGAGQVAKAANQIMVAAQMVAMGELLVFAKKAGVDP
                     RKVVDAIKGGAAQCWTLDVKPPRLFDGNRNPGFKAHMQLKDMGIVLDTAKEYDIPISS
                     AEEHTKFFKMMVEQGMGELDNAAVVGVIEKLAGVEIP"
     gene            complement(794..2299)
                     /locus_tag="PLT08_13405"
     CDS             complement(794..2299)
                     /locus_tag="PLT08_13405"
                     /inference="COORDINATES: protein motif:HMM:NF014425.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000030 - mannosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006493 - protein O-linked glycosylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid carrier-dependent
                     glycosyltransferase"
                     /protein_id="HNE05516.1"
                     /translation="MAIIKEFIRKIHAQQEKHKTWMDGLFLVLCVLYILSGIPLVPFH
                     GDESAYIILSEDYDRVFKKHEVEKVLFDPDGPKQYLRLSTGAILSFSIGFARDITNNE
                     DPINKWLWGASWDENLAKGNLPAPSLLNVARFSSAIMGALGTVLFFVLLRCLYPSRLA
                     AWLATLAFVTQGSLLVSIRRAMQEGPKFLFLTLTTLLAFLIIQNLQTQKLRKALYFAL
                     GIASGLTLAAKQDTAPVLVALYLTIAFIPVWNRTSFRAVWHTLLYLFAATLLAYATFL
                     AFMPVFWQWWESVFVLTGLSVALFQIPFIHETRSKVLASAGLALVVVMTFVSPALWGK
                     LHIPLSSMAELRQSIVGSQVEVSNGQDVLKADSAMGRLNFLLENTISSKVMYMEVDSF
                     NVPPMQASIKAYEASFLSGRTGSFVFDGLIFLLALLGMWFLLRNFNSISLLVYLLFFV
                     TGTFLLVAIPLAWQRYFIIMQLPYMLFAGAGVYQLEMWLNPLFLEKKTASA"
     gene            2319..>2536
                     /locus_tag="PLT08_13410"
     CDS             2319..>2536
                     /locus_tag="PLT08_13410"
                     /inference="COORDINATES: protein motif:HMM:NF012787.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin domain-containing protein"
                     /protein_id="HNE05517.1"
                     /translation="MEINQPVPDFELPDLNGALHRLSNYRGRIVIVNFWSCECPHSER
                     TDKAILSMFVQWQDDVTMLTIASNRSETV"
BASE COUNT          763 a          609 c          584 g          580 t
ORIGIN      
        1 ggttgccgaa ggcgctactg cggcaggttc gcccaaagaa gttgccgaac aagtggatat
       61 tgtttttaca aatttacccg actctcccga tgtagaaaaa gtggcattgg atgaagtcaa
      121 tggaatcatt gcaggcgcaa aacccggcac gatcgtggtg gataactcca ccatcaaacc
      181 ggcgtcggct cgcttgatcg cggcgaaatt aaaagaaaaa gggattttct ctctggatgc
      241 acccgtctct ggcggcgata ttggcgccaa gaatggcaca ctcaccatca tggtcggcgg
      301 cgaagccgaa gcgcttgaaa aagtaatgcc cgtctttcaa gccatgggca agtcggtcac
      361 ccatgtcggc gaggcgggag ctgggcaggt tgccaaagct gccaatcaga tcatggtggc
      421 agcccaaatg gtggcgatgg gcgaattact tgtctttgct aaaaaagcag gcgtcgaccc
      481 gcgtaaagta gtggatgcca tcaagggcgg cgcggctcaa tgctggaccc tcgacgtcaa
      541 accgccgcgt ttgttcgacg gcaaccgcaa ccctggtttc aaagcgcaca tgcaattaaa
      601 agatatgggc atcgtattgg atacggcgaa ggaatacgac attcccatct catcggcaga
      661 agagcacacc aagttcttca agatgatggt tgaacaaggt atgggcgagt tggacaatgc
      721 cgcagtggta ggtgtgattg agaaattggc tggggttgag ataccctaat aaaagctttt
      781 acaaaaacag cctctacgca gaggctgttt tcttttccag gaataaggga ttcaaccaca
      841 tctctagttg atatacgcca gcgcccgcaa aaagcatata aggaagttgc ataatgatga
      901 aatatctttg ccatgccaaa ggaattgcga ccagtaaaaa agtgcctgta acgaaaaaca
      961 agaggtaaac cagaagactg attgaattga aattgcgcaa aagaaaccac attcccaaaa
     1021 gcgccagcaa gaagataagt ccgtcgaaca cgaaagagcc ggtgcgcccg ctcaggaatg
     1081 aagcttcata cgcctttatg cttgcttgca taggcggcac attaaaacta tccacttcca
     1141 tgtacatcac cttggatgaa atggtatttt caagaaggaa attcagccta cccattgcag
     1201 aatccgcctt aagaacgtcc tgcccgtttg aaacctccac ttgagaccct acaatcgatt
     1261 ggcgtaattc tgccatactg ctcaatggga tatgtaattt cccccataat gccggtgata
     1321 caaatgtcat cacaaccacc aatgccagcc cggcacttgc cagcactttc gaacgcgtct
     1381 catggataaa agggatttga aataaagcta cagaaagacc agttaataca aaaacacttt
     1441 cccaccattg ccaaaaaacg ggcataaaag caagaaaggt tgcatatgca agcagagtgg
     1501 cagcgaaaag atataacaag gtatgccaaa cagcccggaa agatgttctg ttccaaactg
     1561 gtatgaacgc aatcgtcaaa tagagcgcca ccaaaaccgg agctgtatct tgcttggcgg
     1621 caagggttaa tccgctggcg atgcctaaag caaagtatag ggctttgcgc agtttctgcg
     1681 tttgaagatt ctgaatgatc aaaaaagcaa gcaaggttgt cagggtcaaa aacaaaaact
     1741 tcggaccttc ttgcattgcc cgccgaatgc taaccaaaag actgccttgc gttacaaagg
     1801 caagggttgc cagccaggct gccaagcgag acggatacaa acaacgcaaa agcacaaaaa
     1861 acagcacagt tcccaaagcg cccataattg cagaactaaa cctggcaaca ttcaacaagc
     1921 ttggtgcagg gagattcccc ttggcaaggt tttcatccca agatgccccc cacaaccact
     1981 tgttgatcgg gtcttcattg ttggtaatat ctcttgcgaa gccaattgaa aatgacaata
     2041 ttgcccccgt agataatcgc aggtattgtt tgggaccatc tgggtcgaac aacacctttt
     2101 ctacttcatg cttcttaaag actctatcgt aatcttcgct caaaataata taagcagatt
     2161 catccccatg aaatggcacc aaaggaatcc cgctaaggat atagagtaca cataacacca
     2221 aaaacaaacc gtccatccat gttttatgtt tttcctgctg agcatggatt tttcttataa
     2281 attcctttat gatcgccatg ttgaacatta tatctaaaat ggaaattaac caacccgtcc
     2341 ctgattttga attgcccgac cttaatggcg ccctccatcg tctttcgaat tatcgcgggc
     2401 gcattgtgat tgtcaatttt tggtcgtgcg aatgtccgca ttccgagcgc acagacaaag
     2461 ccatactgtc catgttcgtg caatggcagg atgatgtgac catgttgact atcgcgtcga
     2521 atcgcagcga aacggt
//
LOCUS       DAOQJE010000563         2531 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_29704_length_2531_cov_4.852585, whole genome shotgun sequence.
ACCESSION   DAOQJE010000563 DAOQJE010000000
VERSION     DAOQJE010000563.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2531)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2531)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2531
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29704_length_2531_cov_4.852585"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(269..1237)
                     /locus_tag="PLT08_13415"
     CDS             complement(269..1237)
                     /locus_tag="PLT08_13415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4350 domain-containing protein"
                     /protein_id="HNE05518.1"
                     /translation="MHKNGRNSNRLFIAAWIPIFGIFLAGCIPGQVPDRSYRPSIAEP
                     AYPQSAGPLVCLDEAHNNFHTLEDRFWAFGELLRKDGYKVEANREKFSAPILAACDVL
                     VISNAQLDIEEWGDYPYPTPSAFSEDEINAVRTWVENGGSLLLIADHMPLAGVASNLA
                     AAFGVTFNDGFAVAGFNDPSQLDAAFDKQTIFSIETKTLIPSVITQGRSASETVTSVR
                     SFTGQAFQAPESAQPVLVLPDDFVLLMPEKAWEFSPDTKTVPVGGWLQGAVLPFGKGR
                     AAFFGEAAMFSAQRSAGEPMGMNAPGAEQNFQLVLNVMHWLSGILN"
     gene            1492..2511
                     /locus_tag="PLT08_13420"
     CDS             1492..2511
                     /locus_tag="PLT08_13420"
                     /inference="COORDINATES: protein motif:HMM:NF022062.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIPR family protein"
                     /protein_id="HNE05519.1"
                     /translation="MSEIHLKRIQTILEKEFSKLIDLSDVEHVSDNDKEKHFLTRAQA
                     ALALSQIADVNNAVASSAIVDGFDDNGIDAIYFDNELRIVYVIQSKWDSTGKKSPDLG
                     SIQKFIQGVKDLLSAKFERFNEKIKAKRDELELALENPEVSFELLIVYTGIQSLSKDG
                     EQVLQDLVDGINDVGDIVNFKAYNQKELYAAISRGMEKASIILENVTLYDFGQILDPY
                     YAVYGQVTSAEIASWYQQHGQQLLARNLRKFKGDTEVNQSIKKTLISETGKFWYFNNG
                     ITVLCDSITKSPKGSPHREVGQFRFNGVSVVNGAQTVGVIATSVSQGFTKAGMTCFGK
                     CDQVK"
BASE COUNT          709 a          525 c          618 g          679 t
ORIGIN      
        1 gtcttgatat cttcaccaat ggtttgacga tctgctttcg gcaattcctt caaccactcg
       61 cggacaggtt ctttgcctgt ttcgctttta tagaatttta cgtgtaggat tggctcccga
      121 tccttgttat catccatagg caagagtgta ccataattga tacatacgtc aacctcaatc
      181 catcctgact tggaaaatca gcacatcaaa aaatataatc tcttctttag tggacacaat
      241 gatcaatacc cgtatggacg tgttgatctc aattcaaaat ccctgagagc caatgcatga
      301 cgttcaagac caattggaag ttttgctctg cgccgggggc gttcattccc atcggttcgc
      361 ccgcggaccg ttgtgcgctg aacatggcgg cttcgccaaa gaaggcggcg cgaccttttc
      421 caaacggcag gacggctccc tgaagccagc cgcctactgg gacggtcttg gtgtcgggcg
      481 agaattccca ggctttttca ggcatgagca gaacaaagtc atctggcagg acgagcacgg
      541 gctgtgcaga ctctggcgct tgaaaggcct gcccggtaaa actgcggaca ctggtcacgg
      601 tttcacttgc tgacctgcct tgtgtgataa cgcttgggat gagcgttttg gtttcgatgg
      661 aaaagatggt ttgtttgtcg aaggctgcat ccagttgact tgggtcattg aacccggcta
      721 cagcgaagcc atcgttgaag gtcacaccaa aggctgcggc gaggtttgaa gcgacaccag
      781 cgagcggcat atggtctgcg atcaggagca gggatccgcc attctcgacc caggttcgaa
      841 ccgcgttgat ctcatcctca ctgaaggcgg agggagttgg gtagggataa tcgccccatt
      901 cttcgatgtc gagttgggcg ttggagatga ccaggacatc acaagccgcg aggatgggtg
      961 cgctgaattt ctcgcggttg gcttcgacct tgtacccgtc ctttcgtaag agttcgccaa
     1021 aggcccagaa gcgatcttcc agggtgtgga agttgttgtg ggcttcgtcc aaacatacga
     1081 gcggacctgc gctttgtgga taggcgggct cggcgatcga aggtcgatac gaccggtctg
     1141 ggacttgccc cgggatgcag cctgccagaa atattccgaa gatgggtatc cacgctgcga
     1201 tgaacaggcg gtttgagttc cttccgtttt tatgcatggc aactcctttg aatattagac
     1261 ctgtattttg gggaattgtt tcaaagaaca gcaagaaaaa aacaccctct acccttcggg
     1321 cacacgtcct aaaggagagg gtcaaacgga gaaatttgat tattaagccc ctcagggtga
     1381 ggtcatgcgg atatgccgat cacgacgctt atcagggtgt tttgaaacaa aatttggtat
     1441 acaatgctta taaatcgtat cttcttgcaa cccaatcaag gagtcttagc aatgagtgaa
     1501 attcatctaa aacgtatcca aaccattctt gaaaaagagt tttcaaaact aattgattta
     1561 tcagatgttg aacacgtttc cgataatgat aaagaaaaac atttcttaac tcgggcgcag
     1621 gcagctcttg ctctttcaca aatcgctgat gtcaataatg cagtagcgtc gagtgcgata
     1681 gtagatggat ttgatgacaa tggaatagat gcaatatatt ttgataatga attaaggatc
     1741 gtttatgtaa tacagtcaaa atgggattcg acaggtaaaa aaagtcccga ccttggatct
     1801 atccaaaaat tcattcaagg ggtcaaagat ttgttatccg caaaatttga acgatttaat
     1861 gaaaaaataa aagctaaacg agatgagtta gagctagccc ttgaaaaccc tgaagttagt
     1921 tttgaattac tgatagttta cacaggcata caatctcttt ctaaggacgg ggaacaggtt
     1981 ttacaagatt tagttgatgg gattaatgat gttggagata tcgttaactt taaagcatac
     2041 aaccaaaagg agttgtatgc tgcgatttcc agaggtatgg agaaggcgtc aataattctt
     2101 gagaacgtaa cgctttatga ttttggtcaa atattagacc cttattatgc tgtatacggg
     2161 caagtgacat ctgctgaaat tgcttcttgg taccaacaac atggtcaaca attattagcc
     2221 cggaatcttc gtaagtttaa aggtgacact gaagtaaatc agtctataaa gaaaacctta
     2281 atcagtgaga ctggtaaatt ttggtatttc aacaatggaa ttactgtttt gtgtgactct
     2341 ataactaaat ctccaaaggg cagcccacat agagaagtag gtcaattcag atttaacggt
     2401 gtatcagttg taaatggcgc acaaactgtt ggtgtaattg caacgtcagt ttcccaaggt
     2461 tttacaaagg caggaatgac ctgctttgga aaatgtgacc aagtaaaatg agagtcgagc
     2521 acctcaaaaa g
//
LOCUS       DAOQJE010000564         2530 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_29725_length_2530_cov_4.291313, whole genome shotgun sequence.
ACCESSION   DAOQJE010000564 DAOQJE010000000
VERSION     DAOQJE010000564.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2530)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2530)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2530
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29725_length_2530_cov_4.291313"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(105..614)
                     /locus_tag="PLT08_13425"
     CDS             complement(105..614)
                     /locus_tag="PLT08_13425"
                     /inference="COORDINATES: protein motif:HMM:NF024698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNE05520.1"
                     /translation="MDLFETQRLLIRHLELTDVDAMFGIFSLPEVTKWMGDGEPLSQE
                     LCAKWIEVSQKNYATKGFGASAVIEKASGEFIGCSGIVYDSERQEPEIIYAFHPSAWR
                     KGYASELVPAMLNYGLTECRLTYILATIAEANKASAHVVEKAGMKFVTEEPNPDGHIT
                     LVYRIEKGK"
     gene            739..1398
                     /locus_tag="PLT08_13430"
     CDS             739..1398
                     /locus_tag="PLT08_13430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE05521.1"
                     /translation="MLNKIFSDPKRIVILILSAIIALFICVVVLIGGGGAILHRVDLT
                     PTTEPVSFGYCGAEFTSLCVVSFGRDALGNTVINLYVPIRRYPIFYLKVVRQSGEQRF
                     ECESNRDVKESVYCTGSPLNLGEGFNIQVVSEKDNIILAEGEFSLIAFRVTTPMVGGV
                     DSINTPGVLNTESTPSIETKKTPTPSQPISESGTVTPTTSDSIVETKTLTPQSSYPSY
                     P"
     gene            1399..>2530
                     /locus_tag="PLT08_13435"
     CDS             1399..>2530
                     /locus_tag="PLT08_13435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE05522.1"
                     /translation="MSVWFDGLSALSDHIWTVLIILVLCFWGVFVIRFFLQRELEKSL
                     TEIELFTLSAVGWIFPLLLLSILTFFVSLFTNLVVGGVFFVLVFFSSTFLIVREKINV
                     LLALKFLLFLIMFIVLRFAFLKNLFLPSYFDSAEHYRLISLINAAYRFGVISSELIDS
                     FYHLGFHFISAFFSVFLQVHVIDFMLVFGQVVLAFLTPALFFVIKRETGSSSSAVLTC
                     VLSTFGFHMPAYLMNWGKYPALSGLIAMLFVFGLAYMIYRKDMFQVRKTFFVFLAGVF
                     LLSVFIHSRTFIVFSAMLIAILVTSLWSHLNSLHRFIGFCVLILVTGVLLNSVKNSVV
                     LRPLLESYFRDDVWILLLVLALTFASLFHYAKQSFFLILWLTI"
BASE COUNT          603 a          486 c          524 g          917 t
ORIGIN      
        1 ctgtcccgcc gatggtgatg aagttgccaa ctttggcgat gtggttgtac ggtccgatcg
       61 gcgtcggggt gttggctggg gtggaggttt caacaatggg catattattt tcctttttca
      121 atacgataga caagggtgat gtgtccatca gggttaggct cttcggtgac aaacttcatg
      181 cctgcttttt ctacaacatg agcagatgct ttattcgctt ctgcgattgt ggcgaggatg
      241 taggtcaacc tgcattcggt caacccatag ttcaacatgg cagggaccag ttcactggcg
      301 tagcctttgc gccatgcgct gggatgaaag gcataaatga tctccggctc ctgccgttct
      361 gagtcgtata caatgccgct gcaaccaatg aactcacctg aagctttttc gatcacagcc
      421 gaagcaccga agcctttggt ggcatagttt ttctgtgaga cttcgatcca cttcgcgcat
      481 agttcttggc ttagtggctc tccatctccc atccattttg ttacttcggg caggctgaaa
      541 ataccaaaca tagcatccac atctgtcaat tcaaggtgcc gaatcaataa acgttgcgtt
      601 tcaaataagt ccatttttgc tcctaagctt tttaagttga aaacattctc tagaacggtt
      661 ctgaaattaa cgttgcttcc gtggtctgca ttatatacat attaatacac agaattttgc
      721 aatggtagaa tgggaataat gctgaataaa attttttcag acccaaaacg tattgtgata
      781 ttaatattaa gcgcaatcat agctctgttt atttgtgttg tcgtgttaat tggcggaggg
      841 ggggcaatat tgcatcgagt ggaccttact ccgactaccg agcctgtatc ttttggctat
      901 tgcggtgcag aattcacaag tttgtgcgtg gtgtcatttg gacgtgatgc attaggcaat
      961 acggttatta atttatatgt accaataagg agatatccaa tcttttattt gaaagtggtt
     1021 aggcaatctg gagaacaaag gtttgagtgc gaatcgaacc gtgatgtaaa agaaagtgtg
     1081 tattgcacgg gctctccatt gaatttaggc gaaggcttca atattcaagt tgtttctgaa
     1141 aaagataata ttatccttgc cgaaggagaa ttttctttga tcgcattccg cgttacaact
     1201 ccaatggtag gaggagtaga ttcgataaat acccctggcg tactcaatac agaatcaaca
     1261 cctagtatag agacgaaaaa aactccgaca ccatctcagc ctatttctga atctggcact
     1321 gttactccga caacatctga cagtattgtt gaaacgaaaa cattgacacc gcaatcttct
     1381 tatccaagtt atccgtaaat gtctgtttgg tttgatggac tttcagcact gtctgaccat
     1441 atttggacag tgcttattat tctcgtttta tgtttttggg gggtgttcgt cattagattc
     1501 tttttgcagc gagaattaga aaagtctctg acagaaatag aactctttac cttaagtgct
     1561 gtaggttgga ttttccctct tctattactt tctattctta ctttttttgt ctcacttttt
     1621 actaacctag ttgttggggg agtattcttt gttttggtat ttttttcttc tacctttctc
     1681 atcgttcgag aaaaaatcaa tgtgctactt gcacttaaat ttcttttatt cttgattatg
     1741 tttattgttt tgcgttttgc atttcttaag aatttatttt taccatcgta ttttgactct
     1801 gcagaacatt atcgtctcat aagcctgatt aatgctgcat atagatttgg tgttatttct
     1861 agcgagttaa ttgatagttt ttatcatttg ggctttcatt tcatttcagc ctttttttct
     1921 gtttttctac aggttcatgt tattgatttt atgctcgtgt ttggacaggt tgtgctagct
     1981 tttttgacac cagccctttt ttttgtgatt aagagggaga ccggatcaag ttcctccgct
     2041 gttttaactt gtgtattgag caccttcggt tttcatatgc cagcatactt gatgaattgg
     2101 ggaaagtacc ccgctttgtc ggggttaata gcaatgctct ttgtctttgg gcttgcctat
     2161 atgatttatc gtaaagacat gtttcaggtt cgtaaaacat tttttgtttt cttggcagga
     2221 gtattcctcc tttctgtttt cattcactcc cgaactttta ttgtttttag cgcaatgctt
     2281 attgcgattc ttgttacatc attatggagc catctgaatt cgttacatcg ctttattggg
     2341 ttttgtgtgc tcattcttgt gactggtgta ttgctaaatt ctgtaaaaaa ctccgtagta
     2401 ttacgccctc ttctcgaaag ctattttcgc gatgatgttt ggatcttgct acttgttctt
     2461 gcgctgacat ttgcatcttt atttcattac gccaaacaaa gctttttttt gattttatgg
     2521 ctgacaataa
//
LOCUS       DAOQJE010000565         2529 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_29744_length_2529_cov_5.631770, whole genome shotgun sequence.
ACCESSION   DAOQJE010000565 DAOQJE010000000
VERSION     DAOQJE010000565.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2529)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2529)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2529
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29744_length_2529_cov_5.631770"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1027
                     /locus_tag="PLT08_13440"
     CDS             <1..1027
                     /locus_tag="PLT08_13440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE05523.1"
                     /translation="FFIQEIIPSAVTNCDCGHKNNSEAWMDGLICGLIIAGIVVISFF
                     FYLLNTARKKGNTDKNQFGKTEYSPVYVSIADKPDSIIRGMNKFVAEARKTETAGDKW
                     RWIPMLIFFAGIGLALIDVFILLLGYSISFVFIGGGVLLWIAAFVMARALRRSDLLDF
                     SPRYNGTKEILHTLRDDLKPGSTFLGHLDLTGSMLQNKVARETQDAQSRTTQLFNDPW
                     LSLKAKLYDGNVLRVTAIQKTKKRKSYWKRSRISGKSKLKPEKFKGSQQELKVRIVVN
                     PEAYVIRPNNQFSLNKDVGKYKVAQLSTEGGMINILAVSPFEDVSHTDILTFLKSAYS
                     LLERKAA"
     gene            1028..>2529
                     /locus_tag="PLT08_13445"
     CDS             1028..>2529
                     /locus_tag="PLT08_13445"
                     /inference="COORDINATES: protein motif:HMM:NF024753.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HNE05524.1"
                     /translation="MSRKIIPFVIALILICLVMGVAGVFAFSGFAAAEKFGSQTAWSQ
                     SYSQAESMKVIDVTGDGQDDLFIQNASEVNILDGSGTPILGLDYPSPKTTLGDVNGDG
                     VEDIVVYYMGPEGASVDVISKGSVSNLAKSLNIGSPARVAFIRFSSGPQIVLGDSSGN
                     VLSIDAGSGAPLWNSNVGSAEIRGMDDARIGGEVHVAIASNDGSVKIFASDGSNVWTV
                     NQEQLRRMRAFDLNADGNSEVITGGEYGAFKIYNAANGQVLFEKSIGQAVSEVREVEL
                     NGDPSSREIVVGGKDGGVWAYSFNGTTASQLWTGSLSDKVTEIAGLDINEDGKQEAVI
                     GDDSGKVAVFTDNGTRNNLPDHSTGITRIDIGKLGSERYVVIADYNEVQTNKVNFSSI
                     PGFQFTPLIVGIIVSAVILAIAGILASIPPKPEMKLSLQDKSRESLEAERRMLKESIA
                     DVERLRKAGEMSNEAYLARLKRLRGDLAENEAAFKTANFPIKTETFACPN"
BASE COUNT          643 a          602 c          644 g          640 t
ORIGIN      
        1 gtttttcatt caagaaatta tacccagcgc ggtcacaaat tgcgactgtg ggcataaaaa
       61 taactcggag gcatggatgg atggtctgat ttgtgggctt atcatcgcgg ggatcgttgt
      121 catctctttt ttcttttacc tgctgaacac cgcacgtaaa aaaggaaaca ccgacaaaaa
      181 tcaattcggc aaaacagagt attcacctgt ctatgtttcg attgcagaca agccagactc
      241 gatcattcgc ggaatgaaca agtttgtggc tgaagcgcga aagaccgaaa cagccgggga
      301 taagtggcgt tggattccga tgctgatctt ctttgcgggc attgggctgg cactcattga
      361 tgtttttatc ttactacttg ggtatagcat ctcctttgtc ttcattggcg gcggtgttct
      421 actttggatc gcagcctttg tgatggcacg tgccttacgc cgcagtgact tgttggattt
      481 ctctccgcgc tataacggga caaaagaaat tctgcacacg cttcgagacg acctcaaacc
      541 tggctctaca ttccttggtc atctcgacct gacgggttcc atgttgcaaa ataaagtagc
      601 ccgcgaaaca caagatgctc aaagccgcac aacccaactt tttaacgacc cttggctttc
      661 gctcaaagcc aagttgtatg atggcaatgt gcttcgtgtt actgccattc aaaagaccaa
      721 aaaacgcaaa tcgtattgga agcgcagtcg tatcagcggc aaatccaagt tgaagcctga
      781 gaagttcaag ggctcacaac aagaattgaa agtgcgcatt gtggtcaacc ccgaggcgta
      841 tgtcattcgc ccgaacaatc aattttcgct aaataaagat gttggcaagt ataaggtcgc
      901 tcagctcagc accgaaggcg gcatgatcaa catccttgct gtttctcctt ttgaagatgt
      961 cagtcatact gatattttga ccttcctcaa atctgcttat tcattattgg aaagaaaggc
     1021 tgcataaatg agtcgcaaaa ttattccgtt cgtcattgca ttaatcttga tctgtcttgt
     1081 catgggggtt gctggcgtgt tcgcttttag cggatttgcc gccgccgaaa aatttggcag
     1141 tcaaaccgca tggtctcaat cctattcgca agcagaaagc atgaaggtca ttgatgtgac
     1201 cggtgatggt caggatgacc tctttattca aaacgcaagc gaggtcaata tcctcgatgg
     1261 gtctggtaca cccattctcg ggctggatta cccctccccc aagaccactc ttggagatgt
     1321 caacggtgat ggggtggaag acattgttgt ttactatatg ggtcccgaag gcgcttctgt
     1381 ggatgtaatc tccaagggca gtgtaagcaa cctggcgaaa tcgcttaaca tcggctcccc
     1441 tgcccgtgtg gcattcattc gcttctctag cggacctcag atcgttctcg gcgacagcag
     1501 cggaaacgtc ctttcgattg atgctgggag cggtgcgccg ctttggaatt caaacgtcgg
     1561 ctcggcagaa atccgcggca tggacgatgc ccgtattggc ggagaagtcc atgtggcgat
     1621 tgcatcgaac gacggttcgg tgaagatctt tgcttcagat ggcagtaatg tttggacggt
     1681 gaatcaggaa caattaagaa ggatgcgtgc attcgacctg aatgccgatg gcaatagcga
     1741 agtcatcaca ggtggtgaat atggcgcctt caaaatctac aacgccgcaa atggacaagt
     1801 tctctttgaa aagtccattg ggcaagctgt gagcgaagtt cgcgaagtgg agttgaatgg
     1861 tgacccatct tcgcgcgaga tcgtagtggg cggcaaagac ggtggtgtat gggcatattc
     1921 gttcaatggc acaaccgctt cgcaattgtg gaccgggtct ttatcagaca aggtgaccga
     1981 gatcgctggc ttagacatca acgaagatgg caagcaagaa gcggtcattg gcgacgactc
     2041 aggcaaggtg gcggttttca cagataatgg cacgcgcaac aacctgcccg accattccac
     2101 tggtatcacc cgcatcgaca ttggcaaact tggcagtgag cgttatgtgg tgattgctga
     2161 ttacaatgaa gtgcaaacga acaaagtgaa cttcagctcg atccccggtt tccaatttac
     2221 accgttgatt gttggcatca ttgtctcggc tgttattttg gcgattgctg gcattcttgc
     2281 ctcgatccca cccaagccag agatgaagct ttcgcttcag gataagagcc gcgaaagcct
     2341 tgaagccgaa cgccgcatgc tcaaagaatc gattgcggat gtggaacgtc tgcgcaaagc
     2401 gggtgaaatg tcgaatgaag cttatctggc gcgcctcaaa cgcctacgcg gagatttagc
     2461 tgagaatgaa gccgccttca agaccgcgaa cttccccatc aaaaccgaaa ccttcgcctg
     2521 ccccaactg
//
LOCUS       DAOQJE010000566         2527 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_29783_length_2527_cov_6.192961, whole genome shotgun sequence.
ACCESSION   DAOQJE010000566 DAOQJE010000000
VERSION     DAOQJE010000566.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2527)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2527)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2527
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29783_length_2527_cov_6.192961"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..241
                     /locus_tag="PLT08_13450"
     CDS             <1..241
                     /locus_tag="PLT08_13450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE05525.1"
                     /translation="KLLGVYSNPDQLVIYKDGSKAFFVVLNFEVEILEGTLGLSNETT
                     DYGYFTLEEMGTMPMHGEHKLRVEDAMRGGDAVMR"
     gene            360..920
                     /locus_tag="PLT08_13455"
     CDS             360..920
                     /locus_tag="PLT08_13455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE05526.1"
                     /translation="MLQNLSSKFYGWAKGWLVLILLVLDGFFGGFLLPLIQGLMQGGS
                     GGIVPLDLMLFSTPDKLFAMLEKYGDSTRIFYRNVELTVDIIYPIIYLFFFGLAISWL
                     FQRGFAPNSPMRKLNVVPLGAWFVDLLENIVIVSLISIFPAKPMALGWVLFLLTTVKW
                     LFAGASILLILVGLVMAIKNGFKKQE"
     gene            complement(962..1654)
                     /locus_tag="PLT08_13460"
     CDS             complement(962..1654)
                     /locus_tag="PLT08_13460"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNE05527.1"
                     /translation="MTTQEEIYKTEGDKYEALIAREDYQGNILKTLREITSLEKRLVL
                     DLGAGTGRLACMLAPHVSHVHAFDISEEMLRVCKQKFAASGLSNWQVDLADHRKLPIP
                     DQSADLVVSGWSLSYLAVWNPDTWREELESWMAEMKRVLKPGSFIVLYESLGTGNEAP
                     IKLDHLKDFYPWLDETGFQNKWIRTDYKFESLAEAEELSRFFFGDELGNKVVQNSWTI
                     LPECTGVWWLKM"
     gene            1740..2150
                     /locus_tag="PLT08_13465"
     CDS             1740..2150
                     /locus_tag="PLT08_13465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE05528.1"
                     /translation="MTTPEHQFDFWLGDWNAAWEGGEGTNHIEMAFDGKVVQENFKAP
                     DLIGMSVSCYDPERTLWCQTWVDNNGSYLDFTGGFENGKMILSRDAIVKGEPCKQRMV
                     WYNITPNEFDWNWERSDDGGKTWQVKWQIKYTRQ"
     gene            2172..>2527
                     /locus_tag="PLT08_13470"
     CDS             2172..>2527
                     /locus_tag="PLT08_13470"
                     /inference="COORDINATES: protein motif:HMM:NF015999.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaK/EbsC family protein"
                     /protein_id="HNE05529.1"
                     /translation="MSTLSPSAQKIQNLLNELGFNYTVIEHVESTRTAQEAADQAGCE
                     LGQIVKSLIFRGKTSGKPILVLTSGPNRVNEKQISEYAGEPIGKADADFVRAVTGYAI
                     GGVPPLGHVEPMETYL"
BASE COUNT          676 a          571 c          588 g          692 t
ORIGIN      
        1 aaaactactt ggtgtgtata gcaaccctga tcagttagtt atttataaag atggaagtaa
       61 ggcgttcttt gtggtgctta atttcgaagt ggaaattctt gaaggaactt taggattaag
      121 caatgagact acagattatg gctacttcac tctggaagaa atgggcacga tgccaatgca
      181 tggagaacat aaattacgcg tagaagacgc catgcgcggc ggagatgcag tgatgcgcta
      241 attttcgcgc gttgccccta caaaagtact gaaaaccaag cctagaacaa tttcatgggt
      301 taatataagc aaccatcatt attcgatgat ggtcgcttat ttttattcac ggaggcagta
      361 tgttacaaaa tctttcaagc aagttttatg gctgggcaaa aggctggctg gttctcattt
      421 tactggtatt ggatggtttc tttggcgggt tccttttgcc gctcattcaa gggttgatgc
      481 aaggcggcag tggcggcatc gttccgctcg acctgatgct tttttccacg cctgataagc
      541 tttttgccat gctcgaaaaa tacggtgact ctacacgcat cttttaccgc aacgtggaat
      601 taaccgtaga tattatctac cctatcattt atttattctt cttcggcttg gcaatctctt
      661 ggctctttca acgtgggttt gcccccaata gccccatgcg caagcttaat gtcgtgccat
      721 taggcgcctg gtttgtggac cttctggaaa acatcgtgat cgtgtctttg atttccattt
      781 tccctgcaaa gccaatggcg ttgggatggg tgttgttcct tctcaccact gtcaaatggc
      841 tgtttgcagg cgcaagcatt ttgttgattt tggtcggctt ggtaatggct atcaagaacg
      901 ggttcaagaa acaagagtaa aaaaatagat ggcagtcact ttagaaatga ctgccatcta
      961 tttacatctt caaccaccat acaccagtgc attctggcaa gatagtccaa ctattttgca
     1021 caactttatt tcccaactca tcgccaaaga aaaagcggga aagttcttcg gcttctgcca
     1081 atgattcaaa tttgtagtct gtgcgaatcc atttattttg gaaacctgtt tcatctaacc
     1141 acggataaaa atctttgagg tgatccaact tgattggagc ctcgttccca gttcccagcg
     1201 attcatacag gacgataaaa ctgcccggct taagcacgcg cttcatttcc gccatccaac
     1261 tttccagctc ttcacgccac gtatccgggt tccagaccgc gagataactt aaactccagc
     1321 cagatacaac caaatccgca gattgatctg gtataggcaa tttgcggtgg tcagcaaggt
     1381 caacctgcca gttggaaagt ccgctggcag cgaatttttg cttacaaacc cgcaacattt
     1441 cctctgagat atcaaaggca tggacgtgac tcacatgcgg ggcaagcata caggcgaggc
     1501 gtccggtacc cgctccgagg tccagaacga ggcgtttttc gagagaggtg atttcgcgca
     1561 gggtcttgag aatgttccct tgataatcct cgcgtgcaat cagggcttcg tacttatccc
     1621 cttcagtctt ataaatttct tcttgtgttg tcatccaaat attatacata aatagttctg
     1681 tggcagggtt tttctcattt cttcttatgc taagcttttt caaaattcaa ggagagaaaa
     1741 tgacaacccc agaacatcaa tttgattttt ggcttggcga ttggaacgcc gcatgggaag
     1801 gcggcgaagg gacaaatcat atagaaatgg cattcgacgg aaaagtagtt caggaaaatt
     1861 tcaaagctcc agacctgatc ggtatgagcg tttcatgcta tgaccctgaa cgaacattgt
     1921 ggtgccaaac ctgggtggat aacaacggct cttatctcga ctttaccggc ggctttgaaa
     1981 acggaaagat gattctcagc cgggacgcga tcgtgaaagg cgagccatgc aaacaacgca
     2041 tggtttggta caacatcaca cccaatgaat ttgactggaa ctgggagcgc tctgacgatg
     2101 gcgggaaaac ctggcaagtg aaatggcaaa ttaaatacac gcgtcaatag gttgttttgt
     2161 ataatgggca gatgtccaca ctttctcctt ctgcccaaaa aattcaaaat ctattaaacg
     2221 agcttggttt caactataca gtgatcgagc acgttgagtc aacacgcact gcgcaagaag
     2281 ccgcagacca agctggctgc gagttgggtc agatcgtcaa gtcactgatc tttcgcggaa
     2341 agaccagcgg taagccaatt ctagtgctta cttcaggtcc aaaccgtgta aacgagaaac
     2401 agatcagcga atatgcaggc gaacccatcg gcaaagcaga tgcggatttt gtccgcgctg
     2461 tgacggggta tgccattggt ggcgtgcctc ccttgggtca tgtagaacca atggaaacct
     2521 atctcga
//
LOCUS       DAOQJE010000567         2524 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_29859_length_2524_cov_5.403807, whole genome shotgun sequence.
ACCESSION   DAOQJE010000567 DAOQJE010000000
VERSION     DAOQJE010000567.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2524)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2524)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2524
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29859_length_2524_cov_5.403807"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            97..1464
                     /locus_tag="PLT08_13475"
     CDS             97..1464
                     /locus_tag="PLT08_13475"
                     /inference="COORDINATES: protein motif:HMM:NF020110.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNE05530.1"
                     /translation="MSLRLRLTLLYSTFMGGILLVFGAAVYILVNVILLNQVDNLLAN
                     VAREIVRATTVDSRGDLNVISLPTLDMTSNAYVQVWGPDGKLITTSPSIGSLTKALDP
                     VGLESNITMYEDSYLDEAHLRVLTVPLKLRSRIIGTLQVAASLTVVDATRTSLLSIMA
                     IIALVAVGLVMWGSWVVLGRALSPLQDIADTVDQINRADDLSRRIPTESAMRDDVGDL
                     VISINQTLERLESLFTSQQRFLADVSHELRTPLTVIKGNVDLIRKFKEADEDLLNSID
                     QEAGRLNRLVSGLLMLAQAESGKLPLNFTQVELDLLLTEVFTETRVLAGSKVRVHLNQ
                     IDEALVKGDRDRLKQVILNLVANAIQYTPQGGEVFLSLKRIGEQVRLIVRDTGPGIPA
                     DDLPYIFERFYRAEKSRTRSKTSGFGLGLSITKWIVEQHGGQIKVESKEGEGTSFVIW
                     LNVLK"
     gene            complement(1480..2175)
                     /locus_tag="PLT08_13480"
     CDS             complement(1480..2175)
                     /locus_tag="PLT08_13480"
                     /inference="COORDINATES: protein motif:HMM:NF013618.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HNE05531.1"
                     /translation="MPRARTTKPYNQTAAIPEDTGSGCFSGFMLVPLTVILISALLGN
                     FALKKTHPLPIIAMDAMPDSTSLKLSPIFTREVLYWADSIVRWASASNLDPNLVATIM
                     QIESCGDPRATSSAGAMGLFQVMPFHFAITDDPYNPETNATRGLNYLVRSLTAANGDV
                     RLAMAGYNGGIGVIPRAEWTWHAETKRYVQYGAPIYTDASSGVSPSNALNEWYSKYGA
                     SLCNQASQRLGLP"
     gene            complement(2251..>2524)
                     /locus_tag="PLT08_13485"
     CDS             complement(2251..>2524)
                     /locus_tag="PLT08_13485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE05532.1"
                     /translation="QPQQQQPRPVYQNQNPAPRPQYNQPTNQPVLRPASQINRPVNAA
                     PASQNTFRPAPKPVSSQQTSFIPPEPTYHPVSYEARSESNERRYDA"
BASE COUNT          577 a          533 c          758 g          656 t
ORIGIN      
        1 gtttttctat tatggtgaga aagatcaggg tatgaaatga tatttctcac catctattct
       61 tagcttgccc taaactcgga caattaaact gtgaccatgt cattgcgttt gcgcctcacg
      121 ttgctatact ccactttcat gggagggatt ttgcttgttt ttggcgcggc ggtttatatt
      181 ctcgtcaatg tcattttgct caaccaagtg gacaatttgt tggcgaatgt ggcgcgtgag
      241 atcgtccgtg caacgacggt cgattcacga ggcgacttaa atgtcattag cctgcccaca
      301 ttggatatga cctcgaacgc ctatgtacag gtttggggac ccgatggaaa gctgattacc
      361 acctctccga gcatcggttc gttgaccaaa gcgcttgacc cggttgggtt agagtcgaat
      421 ataacgatgt atgaagactc ttatctcgat gaagcgcatc tgcgtgtgtt gacggttccg
      481 ctaaaactga ggagccgcat cattgggact ttacaagtgg cagccagcct gacggtggtg
      541 gacgccactc gcaccagtct gctttcgatc atggcgatta ttgctttggt ggcagtgggg
      601 ctggtaatgt ggggctcatg ggtggtgctt ggacgtgcgc tttcaccatt acaagatatt
      661 gctgacactg tagaccagat caaccgcgca gacgacttat cccgccgtat tccgacagaa
      721 agcgccatgc gcgacgatgt gggtgatttg gtcatttcga ttaatcagac cttggaacgt
      781 ttggagtctt tgtttacctc tcaacagcgt tttcttgcgg atgtgagcca tgaattacgt
      841 acgccattga ctgtgatcaa aggaaatgtg gatttgatcc gcaaatttaa agaggcagat
      901 gaagacttgc tcaacagcat agaccaggaa gcaggacgat tgaaccgcct cgtgagcggg
      961 cttttgatgt tggcacaggc agagtctggg aaactgccgt tgaacttcac ccaagtagag
     1021 ctggatttgt tactgaccga agtctttaca gaaacacggg ttttggcggg cagtaaagtg
     1081 cgcgtgcatc ttaatcaaat agatgaagcc ttggtcaagg gtgaccgtga ccgcttgaag
     1141 caagtcattt tgaatttggt ggcaaatgcg attcagtaca caccccaagg tggggaagtg
     1201 ttcttaagcc tcaaacgcat cggggaacag gtgcggctca tcgtccgcga tacaggaccg
     1261 ggaatccctg cagacgattt gccttacatc tttgagcgtt tctatcgcgc ggagaaatct
     1321 cgcactcgct ccaaaacgag cggatttggt ttgggattgt ccattacgaa gtggatcgtt
     1381 gaacagcatg gcggtcagat caaagttgag tcgaaagagg gcgaagggac ttcgtttgtg
     1441 atctggttga atgtcttaaa gtaaacagag ttttggggat tatggcaagc caagtctttg
     1501 actggcttga ttgcagagac tggctccata ttttgaatac cattcattaa gagcattgct
     1561 tgggctaacg ccgctactgg cgtccgtgta gattggcgcg ccatattgca catagcgttt
     1621 cgtttctgca tgccatgtcc attcggcgcg cgggatgaca ccaatgccgc cgttgtatcc
     1681 tgccattgcc aagcggacgt ctccattagc ggcggtcaac gaacggacga gatagttgag
     1741 tccgcgggtg gcgttggttt cggggttgta tgggtcatct gtgatagcaa aatgaaaggg
     1801 catcacttga aagagcccca tggcgccggc gctggatgtg gcgcgagggt ctccgcagga
     1861 ttcgatctgc ataatggtgg caaccaggtt tgggtcgagg tttgaggcgg atgcccagcg
     1921 gacgatagag tcagcccaat agaggacttc ccgtgtaaag atgggggaaa gcttcagaga
     1981 ggtggaatcg ggcatggcat ccattgcgat gattggcagt ggatgagtct ttttcaatgc
     2041 aaagtttcca agcaaggcac tgatgagaat aacggtgagg ggaacgagca tgaagccaga
     2101 aaaacagccg ctccctgtgt cttccgggat ggcggctgtt tgattgtagg gttttgtcgt
     2161 gcgagcgcgg ggcatacgta aggtctcttt tctgggtcag gggacccggg ctcgaaaagg
     2221 tgtattcagt tgtcgaaatg agtaaatgta ttatgcgtcg taacgtcgtt cattgctttc
     2281 agagcgagct tcatacgaaa cgggatgata ggtcggctcg ggcgggatga aggaggtttg
     2341 ctgtgaagag acaggcttgg gcgctgggcg gaaggtgttc tgtgaagcag gcgccgcatt
     2401 caccgggcgg ttgatctgcg aagcagggcg tagaaccggc tgattagtgg gttggttgta
     2461 ctgtgggcgt ggagccgggt tttgattctg ataaaccggg cgaggttgtt gctgttgagg
     2521 ctga
//
LOCUS       DAOQJE010000568         2524 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_29860_length_2524_cov_4.939247, whole genome shotgun sequence.
ACCESSION   DAOQJE010000568 DAOQJE010000000
VERSION     DAOQJE010000568.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2524)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2524)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2524
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29860_length_2524_cov_4.939247"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            8..115
                     /locus_tag="PLT08_13490"
     CDS             8..115
                     /locus_tag="PLT08_13490"
                     /inference="COORDINATES: protein motif:HMM:NF013706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HNE05533.1"
                     /translation="MIFTGTPAGVGELKNGDVVDVEIEGLGKLSNPVKA"
     gene            complement(143..718)
                     /locus_tag="PLT08_13495"
     CDS             complement(143..718)
                     /locus_tag="PLT08_13495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE05534.1"
                     /translation="MHKYLKFFLLENLDTVRRIRVGSFWGVTLLITPWTWLSPFVFFG
                     LHFLLNLLNPRLPLDERLLQSIYFVIAVELTTPLHALGHIISGKLVHSAMDELLITAT
                     RDVNIYHGDQSQISGKVHLVRSLGGPILNILVGLFCILIAPSIPAGFWSGVNTSMIST
                     NLFFGLGGLLPVPSVDGEVIWREIINWMKKK"
     gene            complement(720..1541)
                     /locus_tag="PLT08_13500"
     CDS             complement(720..1541)
                     /locus_tag="PLT08_13500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE05535.1"
                     /translation="MNTKSLAISFPLRVWLAVEVLFGVAAVLAVGLAPADTATNFAWP
                     IQPVVMAAVLGAFYITSAPLFLLPFLAKRWEMVRAMILPAALFSTVQLVATFLHWDKF
                     SVGTFPFYVWFASYLLPPPIFIAVYLWHQKQFGVDAPIPAEDQIPAWLRTMLLVLGIA
                     LSLIAFIAFFLPNVIIPIFPWKLTPLTARSLSGWLVAVGTLMLSIRQGNSRVRSRLSS
                     PMLILILPSLLIQMGRYASEVNWASPTLWFGLLLFALTGFCGLTLAIGNWRESLT"
     gene            complement(1550..1939)
                     /locus_tag="PLT08_13505"
     CDS             complement(1550..1939)
                     /locus_tag="PLT08_13505"
                     /inference="COORDINATES: protein motif:HMM:NF025683.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4383 domain-containing protein"
                     /protein_id="HNE05536.1"
                     /translation="MKSPTRLYTAVAGIFLLLQGVSTLTFRLVPALDAAFPQLLAVTQ
                     MVPTHSILHILTGILALIVLFRGGEKGSLWFSLGFGAFYTSLALYGFITHMPTMFHLQ
                     LFDHPFHLFLGVLGLAAGGFYFYSNRK"
     gene            complement(1936..>2524)
                     /locus_tag="PLT08_13510"
     CDS             complement(1936..>2524)
                     /locus_tag="PLT08_13510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE05537.1"
                     /translation="DEVKTMVPMIAIWTGMLFIVSLFYLGEFDYSRPQVWIWFGAYLA
                     YPSIALWITWSHRTLNEPHDGTPLPAWAKNYLLVQGVLVTLLSLSLLFAPDLMVSVWP
                     WKITRLLAQIYSAPFLSYGLGSLMLSRRQTWKEARVVLIGTVVFAIFVLLASIIHREL
                     FSLSNASTWIWFGGFAIASLVLGGMTVRSLQEKPE"
BASE COUNT          791 a          615 c          651 g          467 t
ORIGIN      
        1 cggtgacttg atctttactg gcacacccgc tggggttggc gagttgaaaa atggtgatgt
       61 ggtcgatgta gaaatcgaag gactgggaaa gttaagtaat ccggtaaaag cataaaaaaa
      121 gccccgcaaa tgcggggctt ttttatttct tcttcatcca attgataatc tctcgccaaa
      181 tcacctcccc atctaccgaa gggacaggca acagaccgcc caacccgaag aatagattgg
      241 tggagatcat gctggtgttc acgccgctcc aaaacccagc gggaatggat ggagcgatga
      301 gtatacaaaa caaacccacc agaatattga gaatgggtcc gcccaacgaa cggacgagat
      361 gaaccttacc ggaaatttgg ctttggtcgc cgtgatagat gttgacatca cgcgtggcag
      421 tgatgagcaa ttcgtccatg gcgctgtgga cgagtttgcc actgatgata tgaccaaggg
      481 catgaagcgg agtagtcaac tcgacggcga tgacaaagta aatggactga agcagccttt
      541 cgtcgagcgg gagccgtggg ttcaaaaggt tgagcaagaa atgcaacccg aaaaaaacga
      601 acggactgag ccatgtccac ggggtgatca gaagagtcac accccagaaa gaaccaactc
      661 gaatccggcg aacggtatca agattttcga gcaaaaagaa tttgaggtac ttgtgcatgt
      721 taggtcaggg actcgcgcca attgccgatg gcaagagtta agccacagaa ccctgtcagc
      781 gcaaaaagca ataaaccgaa ccacaacgtt gggcttgccc aattgacttc actggcatag
      841 cgccccattt gaataagcaa agacggcagg atgagaatca acatgggcga ggaaaggcgt
      901 gagcggacgc ggctatttcc ttggcggatg gaaagcatca gcgtgcccac tgccacaagc
      961 caaccgctca gtgaacgagc cgtcagcggc gttaacttcc acgggaagat ggggatgatg
     1021 acattcggga ggaagaaagc gatgaatgca atgagggata aggcaatgcc cagcactaac
     1081 aacatcgttc tcagccaagc agggatttgg tcttcggcag ggatgggcgc atcaacacca
     1141 aattgctttt gatgccaaag atatactgcg atgaaaattg gaggtggaag tagatagctg
     1201 gcaaaccaaa catagaacgg aaaagtaccg acggaaaatt tatcccaatg caaaaaggtg
     1261 gcgacaagtt gcaccgtgct gaacaacgcc gccgggagga tcatggcgcg gaccatctcc
     1321 caacgtttgg cgagaaaagg caacaagaat aaaggagccg atgtgatata gaacgcgcct
     1381 aaaactgccg ccatcactac aggctgaatg ggccaggcaa agttcgtagc cgtatcggca
     1441 ggggcaagac ctaccgccaa caccgccgcg accccgaaca acacttcaac tgccaaccag
     1501 acacgtaatg gaaatgagat tgctaaactc tttgtgttca tatgaatccc tattttcgat
     1561 tggaataaaa ataaaacccg ccagccgcca agcccagtac gcccaagaac aagtggaagg
     1621 gatggtcaaa aagctgtaag tggaacatgg tcggcatatg ggtgataaac ccatacaatg
     1681 ccaaactggt ataaaacgct ccaaagccga gcgaaaacca caggcttcct ttttcaccgc
     1741 cgcggaacaa gacgatcaat gccagaattc cggtgaggat gtgaagaatg gaatgagtcg
     1801 gaaccatctg cgtcacagcc agtaattgcg gaaaagccgc atccaacgca gggacaagcc
     1861 gaaatgtcaa cgtggatacg ccttgcaata acagaaaaat ccctgcaacg gctgtatata
     1921 aacgagttgg gcttttcatt caggtttctc ctgcaacgaa cggacggtca tgccgcccag
     1981 cacgagagat gcaattgcaa agccaccaaa ccaaatccat gtagaggcgt tggagagcga
     2041 gaaaagttca cgatggatga tcgacgccaa cagcacaaag atggcgaaga ctaccgttcc
     2101 gatcaaaacc acgcgcgctt ctttccatgt ttggcgacga ctaagcatca aacttcccaa
     2161 gccgtacgac aagaaaggtg cggaatagat ttgtgccaac aaacgggtaa ttttccacgg
     2221 ccagacgctc accatcagat cgggcgcgaa tagaagcgac aaagacaata aggtaaccaa
     2281 cacaccttgc acaagcagat aattcttcgc ccatgccggg agtggcgtgc catcatgcgg
     2341 ttcgttcaaa gtgcggtgag accatgtaat ccacaaagcg atcgatggat acgccaaata
     2401 agctccgaac cagatccata cctgtgggcg gctgtaatca aactcgccca aataaaaaag
     2461 cgagacgatg aacaacatac ccgtccagat ggcgatcatc ggcaccatgg tttttacttc
     2521 atca
//
LOCUS       DAOQJE010000569         2521 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_29914_length_2521_cov_3.493512, whole genome shotgun sequence.
ACCESSION   DAOQJE010000569 DAOQJE010000000
VERSION     DAOQJE010000569.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2521)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2521)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2521
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29914_length_2521_cov_3.493512"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1180)
                     /locus_tag="PLT08_13515"
     CDS             complement(<1..1180)
                     /locus_tag="PLT08_13515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE05538.1"
                     /translation="MPNGKILGVKEKFNQFLQKRFVVPATLAILILLAYGILTPWMGF
                     YWDDLPFAWILRFFGPLEFIEGFRPFRPLLGYIFAVTTVLFGGNPLTWQLLGLLVRFF
                     LGLQVWSLLRKVFPTRECSVLWVVLLFTLYPAYGQQWVALTHINQELIPLIFLLFSFI
                     ITVNLLRNGKPSLPFTVLAILFQILGLFSTEYFFGLEVLRFLFVLVILSESTADKKDL
                     FKKSITTWLPYLIVWLLNAAWTYSYHRSDAYNSYQISILSLLSPLALVNEFINTLSLS
                     GFVSWLGTFTIFSSVDGSATQAIAFGILMVAGLVVYVITSNKGQAIIDTEVTHSSSII
                     AHYWFILIGIVTIFAGRLPSWAAGLPLKIEFDYDRFFVSIMLGASLFIIGLADLLLKE
                     S"
     gene            1201..>2521
                     /locus_tag="PLT08_13520"
     CDS             1201..>2521
                     /locus_tag="PLT08_13520"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014425.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HNE05539.1"
                     /translation="MNRSKSLDIAIYIGLIVVFLAPRLPGIGSFVTLDEPSWLSQGAN
                     FYYALGQREFENTVYEYQPAVTTMEIISLGMLLYFPEYRGYGQGYLDYEKGRLDPFMY
                     EHGKDPLVLLIYSRVIQIFVLLILFLALYYLLQKLFPKPVAIFALVFSMFDPFYLSQA
                     RLMNHEGMVTLFVMVSLISLAVYLFKENKFFFLALSGAAAGLAQLSKSSAVAMLAAVG
                     VMLLWKMVQDREQGWGKSFLSVLKSFGIWFGVLVVTYFVFWPGMWVAPGKMLYEVYGN
                     AFSYAFQGARLKITQELDVAEFSLNTRAADIWEVFKILLYRTTPFTWLGVLLGFALPF
                     TRDPEQAPRNRLFFTLLLINAAAFVLLFGIAQGRNSPHYILSSYLSLNLLAGLGWFYF
                     SQWLAERFPSKRMITQYVLLTVVMLAQVLSAVTNYPYYFTYRNPILAA"
BASE COUNT          670 a          472 c          667 g          712 t
ORIGIN      
        1 ggctttcttt caaaagcaaa tctgccaagc cgatgatgaa aagacttgct cccagcatga
       61 tggagacgaa gaaacggtca taatcaaact cgatcttgag ggggagccct gctgcccaag
      121 aggggagacg tccggcaaag atggtgacga taccgatgag aatgaaccag taatgagcaa
      181 taattgatga ggaatgagtg acttccgtat caattattgc ttgtcccttg ttacttgtta
      241 ttacatatac caccaagcca gcaaccatta aaattccgaa ggcgattgct tgtgttgctg
      301 aaccatctac agaagaaaag atggtgaatg tgccaagcca ggagacaaaa ccggaaagag
      361 ataaggtgtt gatgaattcg ttgactaatg ccagaggaga gagcaacgat agaatgctaa
      421 tctggtaaga gttgtaagcg tctgaacggt ggtaggaata ggtccaggca gcattgagga
      481 gccacacgat gaggtagggc agccaggttg tgatggattt tttgaagagg tctttcttat
      541 ccgctgtgga ttcggagagg atgactagga cgaacaagaa gcgcaggact tccaagccga
      601 agaagtattc ggtggagaaa agcccgagaa tttgaaagag aatagcaaga acggtgaaag
      661 ggagagaggg cttgccgttg cgtagaagat ttacggtgat gatgaaagaa aaaagaagaa
      721 agataagcgg aataagttct tggttaatgt gggtgagggc gacccattgc tgtccataag
      781 caggatagag ggtgaagagt aggacgaccc atagaaccga acactcacga gttgggaaaa
      841 ctttccgaag cagcgaccag acttgaagtc caagaaagaa gcggacgagc aagccaagta
      901 attgccatgt gagtggattt ccgccaaata ggacggttgt cacggcaaag atatatccta
      961 ataatggtcg gaatgggcgg aagccttcga taaactcgag gggaccaaaa aagcgcagga
     1021 tccatgcgaa gggtaggtcg tcccagtaga atcccatcca tggtgtgagg atgccatagg
     1081 cgagcagaat gaggatggca agtgtagcgg gaaccacgaa ccgtttttga agaaattggt
     1141 tgaatttttc tttcacacca agaattttac catttggcat gtaacggtat aatccaaact
     1201 atgaaccgaa gtaaatcact agatattgca atttatattg gcttgattgt tgtctttctt
     1261 gcaccgcgat tgccaggcat tggctcgttt gtgacgcttg acgaaccttc atggttgagt
     1321 cagggtgcta atttttatta tgccttggga caacgtgaat ttgaaaatac cgtgtacgag
     1381 tatcaacctg cagtgacgac gatggagatt atttcactcg gaatgttgtt gtattttccg
     1441 gagtatcgcg gatatggtca ggggtatctt gattatgaaa aggggcggct cgaccctttc
     1501 atgtatgagc atggcaaaga tccgttggtt ttgcttatct attcgcgggt gattcaaatt
     1561 tttgttttgt taatactgtt tcttgccctt tattatttgt tacaaaaact cttcccaaag
     1621 ccagttgcta tttttgcact ggtattttcc atgtttgacc cattctactt aagccaagcg
     1681 aggctcatga atcatgaagg catggtgacc ttgtttgtaa tggtttcatt gatctcgctt
     1741 gccgtatatc tgttcaaaga aaataagttc ttctttttag ctctgtctgg tgctgcagct
     1801 gggcttgcac aactttccaa gtcttctgct gttgcgatgt tggcggctgt cggtgttatg
     1861 ttgttgtgga aaatggttca ggaccgcgag cagggatggg gcaaaagctt cttaagcgta
     1921 ctaaaatctt tcggtatttg gtttggtgtt ttggtcgtga cctattttgt tttttggcca
     1981 ggcatgtggg ttgcaccagg caagatgttg tacgaagtgt atggcaatgc tttcagttat
     2041 gcgtttcaag gtgcccgcct taagatcaca caagagttgg atgttgccga gtttagtttg
     2101 aacacgcgtg cagctgatat ttgggaagtg ttcaagattc ttctttacag gacgacgcct
     2161 tttacttggc ttggagtact gttaggattt gcacttccgt tcacacggga cccagagcag
     2221 gctccacgaa accggctttt ctttacactg ttacttatta atgctgcggc atttgtcctg
     2281 ttgtttggta ttgctcaggg gcgtaattct ccgcactaca ttctttcaag ttatttatca
     2341 cttaaccttt tggctggact cggttggttc tacttttcac aatggcttgc ggagcggttc
     2401 ccatcgaaga ggatgatcac tcaatatgta ttgctgactg tggtgatgct ggcacaggtc
     2461 ttgagtgcgg tgacgaatta tccgtattac tttacgtatc gaaatcccat acttgcagcg
     2521 g
//
LOCUS       DAOQJE010000570         2516 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_30020_length_2516_cov_5.340106, whole genome shotgun sequence.
ACCESSION   DAOQJE010000570 DAOQJE010000000
VERSION     DAOQJE010000570.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2516)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2516)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2516
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30020_length_2516_cov_5.340106"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..939)
                     /locus_tag="PLT08_13525"
     CDS             complement(<1..939)
                     /locus_tag="PLT08_13525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE05540.1"
                     /translation="MVSGESTGAKKQILKEGQGSHAHYLLLSLSRAAQAIQQAHTAED
                     FYIAVGREINQLGGEATLLLLDETGTKLSVAYTSYSPKLISRAEKLTGLSLKKYQLPI
                     PSGSVYEIALQGESAVFTDSSNEIISSILPDTLALFSAQIISLFNLRQGILAPLRVDK
                     KVLGLLKINGNFLNMDDLPAMESFAGHIAAGLHNVHLMQKLQEELTAHKQTEQALQHN
                     RNLLLALSQAAHAIQLVRMPDDIYKVVGEQIRALGFEATILKFGSDMNSLHYRYTTIP
                     EKVLRNAEKLAGIKAEDFGWAISQDSIYARILANRRA"
     gene            complement(1198..1650)
                     /locus_tag="PLT08_13530"
     CDS             complement(1198..1650)
                     /locus_tag="PLT08_13530"
                     /inference="COORDINATES: protein motif:HMM:NF024917.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNE05541.1"
                     /translation="MNYSLRSATIQDAPAVTACVNHAFGHYVERIGMKPAPMEMDYEH
                     EIREHQVFVVEDAGQVVGSLVLGITQEGFLLDVIAVEPKYWGKGVGRIMLEHAEAEAK
                     QQGFDSIYLFTHEKMVENQALYKKVGYVEYDRRLEMGRNRVYMRKQLV"
     gene            complement(1685..>2516)
                     /locus_tag="PLT08_13535"
     CDS             complement(1685..>2516)
                     /locus_tag="PLT08_13535"
                     /inference="COORDINATES: protein motif:HMM:NF017984.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0036297 - interstrand cross-link repair
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="crosslink repair DNA glycosylase YcaQ family
                     protein"
                     /protein_id="HNE05542.1"
                     /translation="VKAWSYRGRKETSITLFYLWMVGEVMISNRKGFDRLYDLRERVL
                     PAEYDYAAPTEEAEDFFAKKHIAFSGIAREPSWKTGVGDFIHRKVTAKEGTTWIERLI
                     EKGDIARVGVEGFRENFLVLSSDQAHLSVLEAGRIPKAWKHKGPSTSEEVTFLAPLEI
                     VSARGRAKKVFDFDYVWEVYKPLHQRKWGYYTLPILYGDDLVARMDPKHDRTTNTLQI
                     LGFWLEEDAPRDADFANALAKGLKRFAEMIGATKIDLVGVKPVKLKSELKMLLKRIGF
                     "
BASE COUNT          690 a          613 c          520 g          693 t
ORIGIN      
        1 cgcccttctg ttcgcaagaa tgcgggcata aatactatct tgtgaaattg cccagccaaa
       61 atcttccgct tttattcccg ccaatttttc agcattgcga aggactttct ctgggatggt
      121 tgtgtagcga taatgaaggc tattcatatc gctaccaaac ttaaggatgg ttgcctcaaa
      181 ccccaatgct cgaatctgct ctccaaccac tttataaata tcatctggca tgcgcaccag
      241 ttgaatggca tgggcagctt ggctcaaggc gagtaaaagg ttacggttat gttgaagcgc
      301 ctgctccgtc tgtttatgag ccgttaattc ctcttgcagc ttttgcatta agtggacgtt
      361 atgaagtcca gccgcaatat gcccagcgaa tgactccatt gcagggagat catccatatt
      421 gaggaaattg ccattgatct tgagaagtcc taaaactttt ttatctacac ggagaggggc
      481 aagaatgccc tgacgcaaat tgaacaggga aatgatttga gcggaaaaaa gggcaagcgt
      541 gtctggcaga atagatgaaa taatctcgtt gctggaatca gtaaaaactg ccgattcacc
      601 ctgtaatgca atttcataca cacttccaga cggaatcgga agctgatact tcttcaggga
      661 taacccggtc aacttttctg cccggctgat taattttgga gaataggatg tataagcaac
      721 agaaagtttc gtccctgttt catcaagcag caataaagtg gcttccccac ctaattggtt
      781 gatctctctg cctaccgcaa tataaaaatc ttctgctgta tgtgcctgtt gaattgcttg
      841 cgcagcccgg cttaatgaca gcaaaagata atgcgcatgg ctgccctgtc cctctttcaa
      901 tatctgcttc tttgccccag tggattcacc tgaaaccatc ttggcatcct ctcgaattct
      961 ttttgcctct tctgtatata gaggctgtaa aaaccttgct taggtcttgc gaattcgatg
     1021 atttaaattt tcacattggg agatttaaac aggatggctc agaataaaaa taatttcaat
     1081 gaatgaaaag tcccaatgcc ttgttaatat aaattgtctt gccgttgaag tcaagcaatt
     1141 agaccaaatt ggctgaataa tccgaatggg tggtctaatt tccaaaatcg gtttagtcta
     1201 aacaagttgt ttccgcatgt atacacggtt acgccccatt tcaaggcgac ggtcatattc
     1261 aacatatccg acctttttat acagagcttg attctcaacc atcttctcat gagtaaagag
     1321 atagatacta tcaaaaccct gttgcttggc ttctgcttca gcatgctcca gcatgatgcg
     1381 cccaacacct ttaccccaat attttggctc tactgcgatc acatccagca aaaagccttc
     1441 ttgggtaatt cccaaaacaa gtgagccaac aacctgccca gcatcttcaa caacaaagac
     1501 ctgatgctca cgaatctcat gttcgtaatc catctccata ggagcaggct tcatgccaat
     1561 ccgttccaca taatgaccaa aggcatgatt cacacaagcc gtaacagcgg gagcatcttg
     1621 aatcgtagcg gagcgtaagg agtaattcat gtatcatttt cctaaagata ggttactaag
     1681 aaggtcaaaa acctatccgc ttgagaagca tctttaattc tgatttgagc ttaacaggtt
     1741 ttacaccaac caaatcgatc tttgtagcgc cgatcatctc agcaaaacgc ttcagtccct
     1801 ttgcaagggc gttcgcaaaa tcagcatctc ttggggcatc ttcttccaac caaaagccga
     1861 ggatctgcaa ggtatttgtg gtgcggtcat gctttgggtc catacgtgca accaagtcat
     1921 ctccatataa aatcggcaat gtgtaatagc cccacttgcg ttgatgcaaa ggcttataca
     1981 cttcccacac ataatcaaag tcaaaaactt tcttggcgcg cccgcgagcg ctgacaattt
     2041 ccaacggagc caggaatgtg acctcttccg atgtggatgg acccttatgc ttccacgcct
     2101 tggggatgcg ccccgcctca agaacagaca agtgggcttg gtcgctgctc aacacaagga
     2161 agttttctct gaagccttcc actcctactc gagctatatc acctttttca atgagacgtt
     2221 cgatccatgt ggtgccctct tttgcggtta ctttacgatg gatgaaatca ccgacgccgg
     2281 tcttccatga aggttcgcgc gcaatacctg agaaagcaat atgctttttt gcaaagaaat
     2341 cttcggcttc ttcagtagga gcggcataat catattccgc tggcaagacg cgttcgcgca
     2401 gatcgtagag acggtcaaaa cctttgcggt tagaaatcat cacctcaccc accatccaaa
     2461 ggtaaaacaa ggtgatggat gtttccttac gcccacggta gctccatgct ttgacg
//
LOCUS       DAOQJE010000571         2508 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_30200_length_2508_cov_5.648186, whole genome shotgun sequence.
ACCESSION   DAOQJE010000571 DAOQJE010000000
VERSION     DAOQJE010000571.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2508)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2508)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2508
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30200_length_2508_cov_5.648186"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..347
                     /locus_tag="PLT08_13540"
     CDS             <1..347
                     /locus_tag="PLT08_13540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="SET domain-containing protein-lysine
                     N-methyltransferase"
                     /protein_id="HNE05543.1"
                     /translation="NHSCNPNAGLSGQIGLVAMRDIQIGEEVCFDYAMCDTMPYDEFD
                     CLCGAPNCRGRVGGNDWQRPELQKRYAGFFSPHVQRRIDAQKAERRAFERAMRNAKSG
                     ALAKPVAATMYK"
     gene            complement(415..1083)
                     /locus_tag="PLT08_13545"
     CDS             complement(415..1083)
                     /locus_tag="PLT08_13545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE05544.1"
                     /translation="MHKRLAIVLFVLFSLSIFVTACGGMDQDTEIAVIVALTQTAAAQ
                     QQPQMENTQTEASATVAATEAPVIASTDTPVSVLSGYQPYALCADLNASASANNGLTG
                     TLSSPVPFNDFITGQTGTACQILYSTTNAALPADADPFRATTSMLAAQGWAEDMNYGA
                     GGATGLTTAFRKDSTLCMVGYERRPVDRALCSSDEPIGSCFEKLAPEQIVYTLTLTCS
                     TYFP"
     gene            complement(1202..2428)
                     /locus_tag="PLT08_13550"
     CDS             complement(1202..2428)
                     /locus_tag="PLT08_13550"
                     /inference="COORDINATES: protein motif:HMM:NF024491.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase D-like domain-containing protein"
                     /protein_id="HNE05545.1"
                     /translation="MNKKLAALLIFIFLTACAPLDIETPYTPVTQIPFYASDTPAPFI
                     VPVTETPQIIFPVTETPTLEQPVFVTDTPFPVAKWWEVYFTEPLTINDPNNPAGSIEE
                     KLINFINAAQVSIHIASFEFNLPRVTDALIAAKARGVDVRWVTDDENGLEIDGNANRG
                     QFTRLMAAGVEVKDDAGRTALMHNKFWLFDRQITWTGSTNITVNGIYKQNNNVIVIHS
                     PEVTEIYEREWEELWTAQLGPRAPSTKNNQWAILDGTPLQILFSAEDDAVDSLIAILN
                     DAQVSIRFLAFSFTDYPMAQAMIARAQAGVDVQGIFETFGSNSTRAELKTLWCAGLPV
                     RQDGNSSFLHDKVIIVDNSIVVTGSLNFSSSADEENEENVVILDNPEIAALYLQEYQK
                     LWDQAMSVPTGAFTCE"
BASE COUNT          695 a          588 c          565 g          660 t
ORIGIN      
        1 tcaatcactc gtgcaacccg aacgcaggtc tttcgggcca gattggactc gttgctatgc
       61 gagacatcca aatcggcgag gaagtttgtt tcgattatgc catgtgcgat accatgcctt
      121 acgatgaatt cgactgcctg tgtggtgccc ccaattgccg cgggcgcgta ggcggaaacg
      181 actggcaacg accggaactt cagaaacgct atgcaggttt cttttcaccg catgttcagc
      241 gccgcatcga tgcgcaaaaa gcagaacgac gcgcttttga acgtgccatg cgcaatgcca
      301 agagcggcgc gcttgccaag cctgtagcag caaccatgta taagtaataa ataagattag
      361 acctcggaag taactttccg aggtcttttt tatttaaaaa tcaatatatc aactctatgg
      421 gaaataagtt gaacaagtaa gagttagagt ataaacaatc tgttcagggg ctaatttttc
      481 aaaacaacta cctataggtt catctgaact gcataaagca cggtctacgg gtcttctttc
      541 ataccctacc atacacaacg tagaatcctt gcggaatgca gtcgttaaac cggttgctcc
      601 acctgcgcca taattcatat cttctgccca gccctgagcc gcaagcatgg aagtggttgc
      661 tcggaacggg tctgcatcag caggtaaagc agcattggtt gttgaatata agatctggca
      721 ggctgtgcct gtttgacctg taatgaaatc attaaaagga accggcgaag agagcgtgcc
      781 tgtcaacccg ttgtttgcag aagctgaagc gttcaagtct gcacacaaag catagggttg
      841 gtatccactc aatacactga caggggtatc tgtggaagca ataacaggcg cttcagtcgc
      901 cgccacagta gcagaagctt ccgtttgtgt attctccatt tgaggttgct gttgagctgc
      961 tgccgtttgg gtcaacgcaa caataacggc aatttctgta tcctgatcca tacccccaca
     1021 agctgttaca aaaatagata aagaaaataa aacgaacaga actatagcta gtcttttgtg
     1081 catgcgtgaa gtttcctttt cctacataaa atatgagacg tttctcaacg tctcattcat
     1141 gaatttagtt gcttctttat ctatatttgg gtttatctct aatagcagag ccatttgcaa
     1201 attattcgca agtaaaagca ccagtcggga cgctcatcgc ttgatcccat aatttttgat
     1261 attcctgcaa atacaaggcg gcgatttctg gattatctaa aatcacaacg ttctcttcat
     1321 tctcttcatc tgcactcgat gaaaaattga gcgagcccgt tactacaata ctattatcaa
     1381 caataataac cttgtcatgc aagaaactgc tgttaccatc ttgccgaaca ggcagccctg
     1441 cacaccatag cgtctttaac tcggcacgag tgctattact gccaaaggtc tcaaagatac
     1501 cttgcacatc caccccggct tgggcacgcg caatcatggc ttgtgccatg gggtaatctg
     1561 taaagctaaa ggcaagaaaa cgaatgctca cttgggcatc gttcaaaatt gcaatcaggc
     1621 tatccacagc atcatcttca gcagagaaaa gaatctgcaa tggcgtgcca tctaagattg
     1681 cccattgatt atttttcgta gaaggtgccc gcggacccag ttgtgccgtc cacaattctt
     1741 cccattcgcg ctcataaatc tccgtcactt cgggcgagtg gatcacaatc acattattgt
     1801 tttgcttgta aattccattg acggtgatat tcgtggagcc tgtccatgtg atttgacggt
     1861 cgaacagcca gaatttgtta tgcatcaagg cagtacgccc cgcatcatcc ttgacttcga
     1921 ctccggcagc catcaaacga gtgaactgcc cccgattcgc attgccatct atttccaacc
     1981 cgttttcatc atcggtcacc caacgcacgt ctacaccgcg ggctttggcg gcgatcaaag
     2041 catccgttac gcgtggcaag ttgaattcaa acgaagcaat atgaatgcta acttgagcgg
     2101 cattgataaa gttgatcaat ttttcttcta tagacccggc ggggttattg gggtcattaa
     2161 tcgtcaatgg ttcggtgaaa tacacttccc accattttgc aacggggaaa ggagtatcgg
     2221 ttacaaagac aggttgttca agagtaggcg tctctgtgac gggaaaaatg atctgcggag
     2281 tctcagttac cggaacaata aaaggcgcag gcgtatcact ggcataaaaa ggaatctgtg
     2341 tgaccggtgt atatggggtt tcaatatcga gaggagcaca agcggtaagg aagataaaaa
     2401 tcaggagagc agctagtttc ttgttcatta tgggttcctg aatcaaaagt gacagtcgct
     2461 gtattgtacc ggaaagcgac tgtcactctg agtgacttaa ccgtaaaa
//
LOCUS       DAOQJE010000572         2507 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_30231_length_2507_cov_4.581566, whole genome shotgun sequence.
ACCESSION   DAOQJE010000572 DAOQJE010000000
VERSION     DAOQJE010000572.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2507)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2507)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2507
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30231_length_2507_cov_4.581566"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(82..2043)
                     /locus_tag="PLT08_13555"
     CDS             complement(82..2043)
                     /locus_tag="PLT08_13555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HNE05546.1"
                     /translation="MINSNESVKSPIIERDTNLTPPRTFRSYFIGRPLSTADAPHQTI
                     GKAVGLAVFASDALSSTAYATQEILGVIAAAGTVAYGYLFPISLAIITLLAIVTISYE
                     QTIHAYPGGGGAYIVARDNLGELPAQTAGAALLTDYILTVSVSVSSGVAQIISAYPNL
                     FQYRVWIAVAAVMLIMLINLRGVKESGTAFAIPTYFFVVVMVLTVGYGMYRFFTGSLG
                     AVIDPPHFEAHNVISVITPFILLHAFANGTTALTGVEAISNGITAFKEPRSKNAGITL
                     IWMSLILGSLFLGLSFLTGKVQAVFSEEETVISQLARTIFEGRGALYYMLISGTTVIL
                     IMAANTAFADFPRLGALHAGDGFLPRQLTYRGSRLVYSRGIITLAVIASILIVIFQAS
                     VTRLIPLYAIGVFLSFTLSQAGMARRWWKIGHLKPGEEIVEPGSVLKFEKGWQYRMAI
                     NGFGSLCTAVVMVVFAVTKFREGAWVVLILTPILVTIFFSIHHHYKNVANNLSLDNFG
                     AVPPHQTRHRIIIPISGVHQGSLAALRYARMLSDDVTAVHVAIEPAEADKVKKKWELW
                     GEGTRMVMLNSPYRLFLEPMLEYITEISERRQPGETLTIVVPEFISNNMLGAALHTNT
                     ADLLRSQLKRQEGVVIINVPYQLREHVKN"
     gene            complement(2179..>2507)
                     /locus_tag="PLT08_13560"
     CDS             complement(2179..>2507)
                     /locus_tag="PLT08_13560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HNE05547.1"
                     /translation="LRKQSSAGNEPVFESKGLSVDFARRLVMVNEQEVQLTPTEYDLL
                     KILVTHAGKVITHHQLLRQVWGDGYDDMHILRVNISNLRGKIEPDPSRPTYIHTEPGV
                     GYRLKV"
BASE COUNT          702 a          584 c          649 g          572 t
ORIGIN      
        1 tgtatgaaac aagaaagaca aaaccctcta tccattttgt aaggaagagg gttttgtctt
       61 tgcagttttt tgtttaatta tttaattctt cacatgttca cggagctgat acggaacatt
      121 gatgatgacg accccttcct gacgtttcaa ctggctacgc aatagatctg ccgtgttggt
      181 gtgaagagcc gcacccaaca tattgtttga aatgaactca ggcaccacaa tggtgagggt
      241 ctcgccgggt tggcggcgtt cggagatttc agtgatgtat tccagcatcg gctcaaggaa
      301 gaggcggtat ggcgaattca gcattaccat gcgtgtcccc tctccccata gctcccattt
      361 ctttttaact ttatccgctt cggcgggctc aatggcgaca tggacagcag tgacatcatc
      421 cgaaagcatg cgcgcgtatc gcagtgcagc caatgagcct tgatgaacgc cgctgatcgg
      481 tatgatgatg cgatgacggg tttgatgcgg cggaacggca ccgaaattat caagagagag
      541 gttgttcgcg acattcttat aatggtggtg gatcgagaag aaaatcgtca caaggatggg
      601 agtaaggatc aaaacgaccc atgcaccttc acggaacttg gtcaccgcaa agacgaccat
      661 gacaacagct gtacataacg atccaaagcc attgatggcc atgcggtatt gccagccttt
      721 ttcaaactta agcacagagc ctggctcaac gatctcttca cctggcttaa ggtgaccgat
      781 cttccaccaa cggcgagcca tgcctgcctg tgacaaagta aatgacagga acacaccaat
      841 agcatataga ggaattagcc ttgttacact ggcttggaag ataacgataa ggatggaggc
      901 gatcacagct agagtgatga tgccacgtga gtagacgagg cggctgcccc gatatgtcaa
      961 ctggcgaggc agaaacccat cgccagcgtg aagcgcgcca agacgtggaa agtctgcgaa
     1021 ggcggtattg gctgccatga tgaggatgac tgtcgtgcca gagatcagca tgtaatataa
     1081 agcgcctctg ccctcgaaga tggtacgtgc caactgcgag ataacggtct cttcttcaga
     1141 aaacaccgct tgtacttttc ctgttaagaa ggacaaacca aggaagagcg aaccaaggat
     1201 tagagacatc cagatcaggg tgataccggc attcttgcta cgaggttctt tgaaggcagt
     1261 aataccgttc gaaatggctt ccacgccggt gagagccgta gttccattag cgaaggcgtg
     1321 taataaaata aacggagtaa tgaccgagat gacattgtgc gcttcaaaat gcggcgggtc
     1381 gatcacggct cccagagagc ccgtgaagaa gcgatacatc ccgtacccaa cagtcaatac
     1441 catgaccaca acgaagaaat atgtgggaat ggcaaatgca gtacctgact ctttcacgcc
     1501 gcgcaagttg ataagcatga tgagcataac tgccgcaact gcaatccaaa cacgatattg
     1561 aaataagttg gggtatgccg aaatgatctg tgcaacccca gaggagactg agacagagac
     1621 ggtcagaatg taatcagtca ataaagccgc accagccgtt tgagcgggca actcaccgag
     1681 gttgtcgcgg gcgacgatat aagcaccacc accgccaggg taggcatgga tggtctgttc
     1741 gtacgaaatg gtgacgattg ccaaaagggt gatgatggca agcgagatcg ggaagagata
     1801 accataagct actgtccctg ctgcagcgat aactcctaaa atttcttgtg tagcataggc
     1861 agtggacgat aaagcatcgg atgcgaaaac agccaagcca acagctttgc caatggtctg
     1921 atgcggcgcg tcggctgtgg atagcggtct gccaataaaa tacgaccgaa aggtacgtgg
     1981 gggcgtgagg tttgtgtccc gctcaataat gggggatttt acactttcat tactattaat
     2041 catacagaaa aaattctcct gggatgtgga acgcaaaata tgacacgttc caaacaggaa
     2101 gagtctattc agttgtctgt caaattggca tcaagaaagt atcaagagag gtcaagaaaa
     2161 ggtcaagatt ttatcaattt agaccttcaa cctataccct accccaggct cggtgtggat
     2221 gtaggtcggg cgcgacgggt ctggctcgat ttttccccgc aaattgctga tattgacccg
     2281 caaaatgtgc atatcatcat atccgtcgcc ccacacttgc cgtagcaatt gatgatgtgt
     2341 gatgaccttg cctgcatggg tcaccagaat tttgagcagg tcatattccg tcggcgtaag
     2401 ctggacttcc tgttcattga ccatgaccag acgacgggca aaatccacgc ttaagccttt
     2461 ggattcaaag accggttcat tccccgccga cgattgtttg cgcaacg
//
LOCUS       DAOQJE010000573         2504 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_30298_length_2504_cov_7.002042, whole genome shotgun sequence.
ACCESSION   DAOQJE010000573 DAOQJE010000000
VERSION     DAOQJE010000573.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2504)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2504)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2504
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30298_length_2504_cov_7.002042"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..367
                     /locus_tag="PLT08_13565"
     CDS             <1..367
                     /locus_tag="PLT08_13565"
                     /inference="COORDINATES: protein motif:HMM:NF014342.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DHHA1 domain-containing protein"
                     /protein_id="HNE05548.1"
                     /translation="ALRTQAALSTFNSLLANVQTAKDVNVLGAEIPNADVDTLRSLAD
                     KFREKYPKNGAAVFVSGSTVIATVTEDVVKRGVKAGDLITGIGGKGGGRPNLAQGSLS
                     GDVAAALSRLGQVIEAKVK"
     gene            418..1467
                     /locus_tag="PLT08_13570"
     CDS             418..1467
                     /locus_tag="PLT08_13570"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HNE05549.1"
                     /translation="MLNTSGVKRRQIYLTTFPVGALFVIVFIIVVREKLNFFSEVVGM
                     GSATLILLGCLVLYFQPLKVYTIETSFYFFMALFLPALAGALLTNPNTILNMSSALFA
                     ETINGLTLWVSIIFMGAFLSISPRAFKLLVILVFLVIFLIYVISAVALLQVYKWNFTY
                     LFHFLHSFFALGITMLLISRIGRIQQLHATTDKLTGLLNRYAGSAKLSEEFEYGAGNS
                     TDLAVILVDVDHFKEINDFHGHLVGDKVLKEFGDVISNFIRKTDSVVRWGGEEFLVIL
                     PNTTIENGVRLAERLREEIDQRVFATQLHVTASFGVASRQHGQDMEALFHAADQAMYK
                     AKKGGRNQVVVHPIN"
     gene            1476..2477
                     /locus_tag="PLT08_13575"
     CDS             1476..2477
                     /locus_tag="PLT08_13575"
                     /inference="COORDINATES: protein motif:HMM:NF039236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske 2Fe-2S domain-containing protein"
                     /protein_id="HNE05550.1"
                     /translation="MNPFPAIQNAWYIACRSSQLKRKPITCTILNTPFVLFRNASGSA
                     AALIDRCSHRNVALSDGWVKDGCVVCPYHGWEFDGEGTCQNVPGLMHERPQKARNVSA
                     YPVVEQDGMIWVYPSLETPTSQPPRFEFLHEPGFTHIVAERTLPVALPDALENFLDAT
                     HTHFVHSGLIRSEGKRKAVNITVQREADRVQAEYIDEGTQSGLISRLFGAGIDKSFGR
                     YIFPSTAQLEYRAGENTKMLITLCFTPETETSQRLYAVVVGRGPAIFKPIIQLLFLQA
                     YQQDKHILELQRENLQKFEKTQYVFTDADVLLPHILHIMKHGPTPVTASTRTVKLLL"
BASE COUNT          647 a          555 c          573 g          729 t
ORIGIN      
        1 tgccttgcgc acccaggctg cactttctac attcaactca ctccttgcga atgttcaaac
       61 cgccaaagat gtcaacgttc tcggcgcgga aattcccaat gcggacgtgg atacgctccg
      121 ctcgctggca gataagttcc gcgagaagta tcccaagaat ggcgcggcag ttttcgtttc
      181 gggcagcact gtcattgcca ccgtgaccga agatgtggtc aaacggggag tcaaggctgg
      241 ggacttgatc acaggcatcg gcggcaaggg aggcggtcgc cccaatctcg ctcagggtag
      301 tttgagcggt gatgtcgctg cggcacttag tcgcctcgga caggttattg aagcaaaggt
      361 caagtaaatt taaagggtgg ttcgccaccc tttttgctta ctaggattga tccgtctatg
      421 ttaaatacgt ctggcgttaa acggcgacaa atctatttaa caacttttcc ggtaggagca
      481 ctctttgtta tagtgtttat tattgttgtc cgtgaaaagt taaacttctt cagcgaagta
      541 gtagggatgg gttcggcaac actgattttg ctgggttgct tggtcttgta ctttcaacca
      601 ctaaaagtgt atacaatcga aacgtcattc tattttttca tggcgttgtt tttacctgca
      661 cttgcggggg cgttactaac aaatccaaat actattttaa atatgtcatc ggcattattt
      721 gccgagacta ttaacggttt gaccctttgg gtatccataa tttttatggg tgcttttctt
      781 tcgatttccc caagggcatt taaactgtta gtgatccttg tttttttagt catatttttg
      841 atttatgtga tctcggcagt tgctttgctg caggtttaca aatggaattt tacgtattta
      901 ttccattttc tacactcttt ctttgcatta ggaattacga tgcttttgat ctcgcggatc
      961 ggacgaatac aacagctcca tgcaacaacg gataaattaa ccgggttgct taatcgatat
     1021 gcaggttctg ctaaactaag cgaagagttc gaatatggcg caggaaattc aacagatctt
     1081 gcggttattc tggtagatgt tgatcatttc aaagaaataa atgattttca tggacatctt
     1141 gtaggagaca aagtcctgaa agaatttggc gatgtgatat ccaatttcat tcgcaagaca
     1201 gactctgtag ttcgttgggg tggcgaagag tttttggtta ttttgccgaa tacaaccatt
     1261 gaaaatggag tgaggcttgc tgaaaggctg cgtgaggaga tcgatcaacg agtttttgct
     1321 acacaacttc atgtaactgc cagctttggt gtagcatcac gccaacatgg gcaggatatg
     1381 gaagccttgt tccatgctgc tgaccaggca atgtataaag cgaaaaaagg tgggcgaaat
     1441 caagtagttg tacaccctat aaattgagcg tgctgatgaa tccatttcct gccatacaaa
     1501 atgcctggta tattgcctgc cgttcctccc aactgaagcg taagcccatt acctgcacta
     1561 tcctaaatac accctttgtt ttattccgca acgcaagcgg aagcgcagcc gcattaatag
     1621 accgctgttc gcaccgcaat gtcgccttgt ctgatggctg ggtaaaagat ggttgcgtgg
     1681 tctgcccgta tcacggctgg gaatttgatg gagaaggaac ttgccaaaac gtgcccggtt
     1741 tgatgcatga gcgacctcaa aaggctcgca acgtttctgc ttatccagta gtagaacaag
     1801 atgggatgat ttgggtttat ccctcactag aaacacctac atctcagccg ccgcgttttg
     1861 aatttttaca tgaacccggc tttacacata ttgttgcaga acgtacattg ccagttgcac
     1921 ttccagatgc actggaaaac tttttagatg ccacacatac gcatttcgtc cactctgggc
     1981 ttattcgcag cgaagggaaa cgcaaggcgg ttaatatcac tgtccaacgt gaggctgatc
     2041 gtgtgcaagc agaatacatc gacgaaggaa cacaatctgg gttgatctca cgcctctttg
     2101 gtgcaggaat cgataaaagt tttggacgtt atatttttcc ttctacggcg caattggaat
     2161 accgtgctgg tgaaaacact aaaatgctga tcactctttg tttcacccct gaaaccgaaa
     2221 cctcgcaaag attgtatgca gttgtagttg ggcgcggacc agctattttt aagcccatca
     2281 ttcaactctt attcttgcag gcgtatcagc aggataaaca tattctggaa ctacaacgag
     2341 aaaacctgca aaaatttgaa aaaactcaat acgtatttac cgatgcagat gttctgcttc
     2401 cgcacattct tcacatcatg aagcatggac ctactcccgt caccgcttcc acccggaccg
     2461 tgaagctgct tctgtaattc tgatagatgt cctctcctgt aact
//
LOCUS       DAOQJE010000574         2499 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_30418_length_2499_cov_4.430442, whole genome shotgun sequence.
ACCESSION   DAOQJE010000574 DAOQJE010000000
VERSION     DAOQJE010000574.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2499)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2499)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2499
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30418_length_2499_cov_4.430442"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..919)
                     /locus_tag="PLT08_13580"
     CDS             complement(<1..919)
                     /locus_tag="PLT08_13580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559307.1"
                     /note="catalyses the hydrolysis of terminal non-reducing
                     alpha-D-galactose residues in alpha-D-galactosides;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase/alpha-galactosidase"
                     /protein_id="HNE05551.1"
                     /translation="MPKITFIGAGSTVFAKNLLADILAFPELANSTISLMDIDPVRLK
                     TTEIVAHKIAEFFGAKPKIEATIDRRAALDGADYAISMFQVGGYKPSTVIDFEIPKKY
                     GIKQTIADTLGIGGIMRGLRTIPVFLDICKDMEEVCPDVTFLQYVNPMAMNTWAINKA
                     SKIKTVGLCHSVQGTAEVLAEDIGVPINEINYICAGINHMAFYLKFERNGEDLYPKIR
                     KAAEAKSYARGYRNLANHVRYEVFKRTGYFVTESSEHFSEYVPWFIKHNRPDLLEEFE
                     IPVDEYITRCEIQIAEWEADKAALERGELN"
     gene            complement(981..1523)
                     /locus_tag="PLT08_13585"
     CDS             complement(981..1523)
                     /locus_tag="PLT08_13585"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024917.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNE05552.1"
                     /translation="MTELQVRFVPKAEITSELDSEIDALDKLAFADDGIDHDPEFSSI
                     HWAEPDWMALGFLGEELVTQLCLPKREISVGTEKVWVAGIGGMATHPKHHHKGYGSKL
                     LIATETFMRNEMCVPFGLLICADETRPFYELARWQVAANWLAYHQDNERKILRTCVMI
                     LQLQDQTWLAGEIDLCGAPW"
     gene            complement(1520..>2499)
                     /locus_tag="PLT08_13590"
     CDS             complement(1520..>2499)
                     /locus_tag="PLT08_13590"
                     /inference="COORDINATES: protein motif:HMM:NF027442.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF4832 domain-containing protein"
                     /protein_id="HNE05553.1"
                     /translation="LKHIEQLTPLLQSNTDVIAWVEAGFIGAWGEWHTSTNGLDNIQD
                     KQEILNAILNAIPNRFVQVRYPANIIEMYPNPEDAVQARVAHHNDCFLSSETDVGTYE
                     RNGEITIERDLAYLAELTRFTPMSGESCAPFPPRSECANAVQEMELLHFSAINEAYHK
                     GILRSWEDGGCLQEIADRMGYRLALTSADFNDQVRPGGILNLSVTLENKGFASIINER
                     FLYIVLFNGSERYEVKTSIDPRAWQPGTSSFDIQLHVPSNVDEGEYQLALWLPDAYET
                     LHDDPLYAIQFANENTFDEATGYNILGIIKVDKNTSGNYQHGEEFEVIK"
BASE COUNT          559 a          578 c          683 g          679 t
ORIGIN      
        1 cgttcagttc gccgcgttcg agggcggctt tatcggcttc ccattcggcg atctgaatct
       61 cacagcgggt gatgtattcg tcgacgggaa tttcaaattc ttcgagcagg tcgggacgat
      121 tgtgtttgat gaaccacggc acatattcgc tgaagtgttc actcgactcg gtgacgaaat
      181 agcccgtgcg tttgaagact tcatagcgga catggttggc aaggttgcga tatccgcgtg
      241 cataggattt tgcctctgcg gctttacgaa tttttggata gaggtcttca ccattgcgct
      301 cgaatttgag atagaacgcc atgtggttga tgcccgcgca gatgtaattg atctcattga
      361 tcggcacgcc aatatcttcg gcaagaactt cggctgtgcc ttgcacgctg tgacacaagc
      421 cgacggtctt gatcttgctg gctttgttga tcgcccaagt gttcattgcc atcgggttga
      481 cgtattgcag gaaggtcaca tcggggcaga cttcttccat gtctttgcag atgtcgagaa
      541 agacggggat ggtgcgtagt ccgcgcatga tgccgccaat gccaagcgta tcggcgatgg
      601 tttgtttgat gccatatttc ttcgggattt caaaatcaat gacggtgctg ggtttgtagc
      661 cgccaacctg gaacatactg atggcgtagt ccgcaccatc gagtgcggcg cggcggtcga
      721 tggtggcttc gatcttcggt ttcgcgccga agaactctgc gatcttgtgc gccacgattt
      781 cggtggtttt gaggcggacg gggtcaatgt ccatcaggct gatggtggag tttgcaagct
      841 cggggaaggc gagaatatct gcgagcaggt tcttggcgaa gacggtgctg cctgcgccga
      901 tgaaagtgat tttgggcatg aggttctcct agttagtatc aagtgtttat gtttcaggtt
      961 tgatctacga ttgcatttga ttaccacggt gcgccacaca ggtcaatttc acctgcaagc
     1021 caagtttgat cttgcaactg caagatcatt acacacgtgc gcaatatctt tcgttcattg
     1081 tcttgatgat aggcgagcca gtttgcggcg acttgccagc gagcaagttc ataaaacgga
     1141 cgcgtctcgt ctgcacagat gagcaaacca aatggtacac acatctcatt tcgcataaaa
     1201 gtttctgtcg ctataagcaa ctttgagcca tatcccttat ggtggtgctt tggatgcgtc
     1261 gccataccgc cgatgcctgc cacccaaact ttttcagtgc cgacggaaat ctctcgcttg
     1321 ggaaggcaca attgagtaac taattcttca cctagaaaac caagtgccat ccaatcaggc
     1381 tctgcccaat ggatgctgga aaactcagga tcatggtcaa tgccatcgtc ggcaaaagcg
     1441 agtttatcca gggcgtcgat ttcactgtca agttctgatg tgatttctgc tttgggaaca
     1501 aagcggactt gcaactcggt catttgataa cctcaaattc ttctccatgt tgataattgc
     1561 cgcttgtatt tttgtccact ttaatgatgc ctagaatgtt gtagcctgtg gcttcatcga
     1621 aggtattttc gttggcgaat tgaatggcgt agagcggatc gtcatgcaat gtttcatagg
     1681 catcaggaag ccagagcgcg agttggtact cgccttcgtc tacattggag gggacatgca
     1741 gttgaatatc gaacgatgaa gttccaggct gccatgcacg cgggtctatg gaggttttaa
     1801 cttcatagcg ttcggaaccg ttgaagagaa caatatataa aaacctttca ttgatgattg
     1861 aagcaaatcc tttgttctct aaagttacag agagattgag aatgccgcct ggacgcactt
     1921 gatcattgaa atctgctgag gtcaatgcga ggcggtagcc catgcgatct gcgatctctt
     1981 gcaagcagcc gccatcttcc cacgaacgca ggatgccttt gtgatacgcc tcattgatcg
     2041 cagagaagtg cagcagttcc atttcttgaa cggcattcgc gcattccgaa cgcggcggga
     2101 agggcgcgca agattctccg ctcataggcg tgaagcgggt caactcggcg aggtaagcca
     2161 ggtcacgttc gatggtaatt tcgccgttcc gttcgtaggt gccaacatcg gtttcgctgg
     2221 aaagaaaaca atcgttgtga tgcgccactc gcgcctgtac tgcgtcttca gggttgggat
     2281 acatttcaat gatgttggcg gggtagcgca cttgaacaaa gcggttcggg atggcgttaa
     2341 gaatcgcgtt gagaatttcc tgcttgtctt gaatgttatc cagtccattc gtggaggtat
     2401 gccactcgcc ccatgcgccg atgaagcctg cttcgaccca ggcgatgaca tccgtgttgg
     2461 attgcaggag tggggtcaac tgctcgatgt gtttgagga
//
LOCUS       DAOQJE010000575         2498 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_30430_length_2498_cov_3.731478, whole genome shotgun sequence.
ACCESSION   DAOQJE010000575 DAOQJE010000000
VERSION     DAOQJE010000575.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2498)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2498)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2498
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30430_length_2498_cov_3.731478"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..594)
                     /locus_tag="PLT08_13595"
     CDS             complement(<1..594)
                     /locus_tag="PLT08_13595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE05554.1"
                     /translation="MRTFLENGFKAFLKAASWNWKPYSRLILYGDSVGWSLDWDMREL
                     ANISRRLSVRLVRPHWKHAATPQSFFFASQFFLEDDAWLRFLPGRIAFSYFHGLPNTG
                     DKLMDRVYNGLRIHHEKLARIQVTHSEMREVTLSTGIDPAKVFTIPIGINLDYFPWQT
                     AEAKKHNRERLGIPQSAFVVGSFQKDGNGWGDGLEPKL"
     gene            complement(594..1616)
                     /locus_tag="PLT08_13600"
     CDS             complement(594..1616)
                     /locus_tag="PLT08_13600"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNE05555.1"
                     /translation="MRIPELDKNVIVRRNKLGMGSIVNFARDGIAFLTGFVTSADLSI
                     FHDFEPPPTGGGHQFLRAFWGQVEARGLRVENNLISRTTRACLFNSFNFRESLLKFTK
                     RDSVLYVHRVDGPIDVYRGRDDGVDRGIQAVNRRFAAKTIFQSNYSLEKHLELGMQFH
                     NPVVIMNAADPDIFHSSGRGAFSRDRKTRLIASSWSDNINKGAPVYQWLDEHLDWERY
                     EMTFVGRSPVQFRNIHMVSAVDSYRMAELFRTHDVYLTASKNDPCSNSLIEALTCGLP
                     AVYLQSGGHPEIVKQAGAGFEAAEQIPDLLNKLVSDYEKYQSQISVPNIQEVSAEYLK
                     VLELLK"
     gene            complement(1707..2426)
                     /locus_tag="PLT08_13605"
     CDS             complement(1707..2426)
                     /locus_tag="PLT08_13605"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNE05556.1"
                     /translation="MQTNEKKTLTIMIPAYGEAENLRVLLPSIKEITKDLAVPSEIIV
                     LDAESPVDDTQAVCVENQVRWVSRTGGSRYGDAIRTGIRSCTGDFVITMDADGSHNPQ
                     FIYKMWEKRLDADVVIASRYIPGGVTENPWLLVAMSRILNITFKFFVGMPVYDISNSF
                     RLYHGDLLRSIEPKYSDFDILEEILAKIIWGNFTPPAVIIEIPFRFEQRKYGKPKRNL
                     LVFTAQFLIALFKLRWLRFQK"
BASE COUNT          747 a          621 c          494 g          636 t
ORIGIN      
        1 caatttgggc tccaagccat ctccccagcc attcccatcc ttttggaaag accccaccac
       61 aaacgccgat tgagggatgc ccaatctttc ccgattgtgt ttctttgcct ctgctgtttg
      121 ccagggaaag taatcaagat taatgccaat gggaatcgta aacaccttgg ctgggtctat
      181 acctgtgctc aatgtcactt cacgcatctc agaatgggta acttgaatac gggcaagctt
      241 ttcatgatga attcgcaatc cattatagac acggtccatt aatttatcac cggtgtttgg
      301 cagcccatga aaataagaaa aagcaatacg acccggtaaa aaacgcaacc aggcgtcatc
      361 ttcaagaaag aactgactgg caaagaaaaa agattgaggc gttgcagcat gcttccaatg
      421 cgggcgcact aaacgcacac tcagcctgcg ggaaatattc gccaattcgc gcatatccca
      481 atccaatgac cagccaacac tatccccata taagatcaag cgggaatatg gcttccaatt
      541 ccatgaagcc gctttaagaa atgccttgaa gccattttca agaaatgttc tcatcacttt
      601 aacaattcca acactttcaa atactcagca cttacttcct gaatgtttgg aaccgatatc
      661 tgggattgat acttttcgta atccgaaacc aatttgttta gcagatcagg aatttgctcc
      721 gccgcttcaa accccgcccc agcctgcttc acgatctccg ggtgaccgcc actctgaagg
      781 taaaccgccg gaagaccgca tgtcaacgct tcgatcaacg agttggaaca agggtcattt
      841 ttactggcag tcagataaac atcatgagta cggaacaatt ctgccatacg atacgaatcg
      901 acagccgata ccatgtgaat atttctgaat tgcaccgggc tgcgtcctac gaatgtcatt
      961 tcgtatcgtt cccaatcaag gtgttcatcc aaccattgat agactggtgc gcccttgttg
     1021 atattatcag accagctgga tgcgatcagg cgggtctttc tatcacgcga gaaagcccct
     1081 cttccactgg aatggaatat atcggggtcg gcagcattca tgatcacaac aggattatga
     1141 aattgcatcc caagttctaa atgcttttca agactatagt tagattgaaa aatggttttg
     1201 gcagcaaacc gtcggttcac tgcttgaatc ccacgatcca ccccatcgtc ccgcccgcgg
     1261 taaacatcaa tcggtccatc aactcgatga acataaagaa cagaatcccg ctttgtaaac
     1321 ttaagaagag actccctaaa attaaaagaa ttaaacagac aagcgcgagt cgtgcgtgaa
     1381 atcaggttat tttcgacccg aagtccccgc gcttccacct gcccccagaa cgcacgcagg
     1441 aattgatgcc cgcctcctgt tgggggtggt tcaaaatcat ggaagatgga aaggtcagcg
     1501 gaggtgacaa accctgtaag aaaggcaatt ccgtctctgg caaaattcac aatgcttccc
     1561 attcccaatt tatttctgcg aacgatcaca ttcttatcca attcaggaat tctcaagaga
     1621 ggtacacttc aagttatctg gcatagtcaa tatacatcag ggttatagtc caactcaatg
     1681 atcaagatca aatatttcct tacgttttat ttctgaaacc gaagccagcg caacttaaac
     1741 aaagcgatca aaaattgggc agtaaaaaca agaaggttcc gcttcggttt accgtacttt
     1801 ctttgttcaa atcgaaaagg gatttcaata ataacggcag gcggcgtaaa atttccccaa
     1861 ataatcttgg caagaatttc ttcaagaata tcaaagtcgg agtactttgg ttcgatactg
     1921 cgcaataaat ctccatgata cagcctgaaa ctattggaaa tatcgtaaac aggcatacca
     1981 acaaagaatt taaaagtaat gtttaaaatg cggctcatgg caaccagaag ccaggggttt
     2041 tccgtaactc cgccaggaat atagcgggac gcaatcacta catcagcatc taggcgtttt
     2101 tcccacattt tgtaaataaa ctgtggattg tgcgagccat cagcatccat ggtaatgaca
     2161 aaatcacctg tacaggaacg aatacccgta cgaatagcat ccccataacg gctcccccct
     2221 gtacgagaga cccaacgaac ttgattttca acacacactg cctgcgtatc gtcaacagga
     2281 gactcggcat ctaaaacaat gatttcagat ggcacagcga ggtctttcgt tatttccttg
     2341 atggaaggca acaaaactct aaggttttcc gcctcgccat aagcggggat catgatcgtt
     2401 aacgttttct tttcattggt ttgcatgaaa aatctctcta ctccaatcca tcatttctcg
     2461 aatgccatcc cgttttgaaa ctgttggcgt ccaattaa
//
LOCUS       DAOQJE010000576         2497 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_30446_length_2497_cov_4.288698, whole genome shotgun sequence.
ACCESSION   DAOQJE010000576 DAOQJE010000000
VERSION     DAOQJE010000576.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2497)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2497)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2497
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30446_length_2497_cov_4.288698"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(125..355)
                     /locus_tag="PLT08_13610"
     CDS             complement(125..355)
                     /locus_tag="PLT08_13610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE05557.1"
                     /translation="MNDLEKIIMEEVSTLDDMRLIDVIGFIRYLKAEKPIRQEWIAGW
                     YESAIKSMHEREEELRITPAELEKQLRKKQSQ"
     gene            556..1857
                     /locus_tag="PLT08_13615"
     CDS             556..1857
                     /locus_tag="PLT08_13615"
                     /inference="COORDINATES: protein motif:HMM:NF017760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNE05558.1"
                     /translation="MEKPIERPSILQTYTRSFEPLKNANLRMYLGGQAISLLGTWMQS
                     TAQSWVVWELTQSESALGIVVMLNSLPLFLLAPWAGGFADRFNRRAILMVTQATAMLL
                     AFALAFLVQTKLDQIWHIYVFSFVLGTIAALDFPAQQAFIGDLSGMKQVRQAVTLNIM
                     GLQLARMLGPAAAGFLLKAVGSAAAFWINGASFLVVLFSIFMVKSHQQEIHRRENGRG
                     QFREVLDYVRAQPRMVDLLIFASLVTFFGLSIMNILPAVADDVLKGDSQTFGLLMGAS
                     GAGALISTLFLLPLSQQAKRIGRIVGGAAVWMGTFYLLLSRSTYLPLSMLSIFLVSLG
                     APLVLTTGLGVMQLMAPGDMRARIISLFTMVSFGLQPLSSLIIGYAAETFGTQTIIMI
                     NAACLTLGGILMLMLRKGLIQWELKPMVEPILSSQEKAAMH"
     gene            complement(1925..>2497)
                     /locus_tag="PLT08_13620"
     CDS             complement(1925..>2497)
                     /locus_tag="PLT08_13620"
                     /inference="COORDINATES: protein motif:HMM:NF014693.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FmdE family protein"
                     /protein_id="HNE05559.1"
                     /translation="EKSMKDHDHLCPRQILGVRIGLAGMKALGFSEPPQHKQMLVISE
                     IDGCFVDGVIAATDCTVGHRTLRVEDYGKVAVTFVHVKTGRAVRIAPALDIREQAALF
                     VPSEPRHYFAQMQAYQTMPDETMLNITEVELTTPIQEIVSRPGVRVNCDVCGEEIMNE
                     REVHSNGHIFCQSCYSHESYYHSVIRQKFP"
BASE COUNT          574 a          611 c          559 g          753 t
ORIGIN      
        1 gtttgatgcc gggaaaagct cgtggaatga ttctggcagg tgtgcgaagt gtggtcatgc
       61 ttttggatat ggatcctaca ttgattttag cacgggcaga ttatctgtta ttcacccatt
      121 aggactattg actttgcttt tttcttaact gtttttcaag ttctgcggga gtaatcctta
      181 attcttcttc gcgctcgtgc atagacttaa ttgcactttc ataccagcct gcaatccact
      241 cctgccgaat gggcttttct gccttgagat agcggataaa cccgatcaca tcgattagac
      301 gcatatcatc caaggtggaa acctcttcca tgataatttt ttctaggtcg ttcataatag
      361 ctcctatact ttgaatacaa acagtcattg gagaaatata taggatttcc tcgacattgt
      421 tttgaaagga aatcggttta tagttaaggg gatttcccaa attgtacttt gttttattaa
      481 tgattggtaa gaattcagct tgggatttca tccgaattct tgtctaaaat ctcttcaatc
      541 ccttaggagc cttccttgga aaaaccaatt gaacgtcctt ccattcttca aacttacact
      601 cgtagtttcg agccgcttaa gaatgccaat cttcgcatgt acttgggtgg gcaagcgatt
      661 tctctgcttg gtacgtggat gcaatctaca gcacaaagtt gggtggtttg ggagttgact
      721 caatctgaaa gcgcgcttgg catcgtggtc atgctcaata gtttgcctct cttcttgctt
      781 gcgccatggg cgggtggctt tgcagaccgt ttcaatcgcc gcgcgattct catggttact
      841 caagccaccg ccatgcttct agcttttgcc ttggcgtttc tagtgcaaac caagcttgat
      901 caaatctggc atatttacgt cttttcattc gtccttggca ccatcgccgc gttagacttc
      961 cccgcccaac aagcatttat cggcgatctc tctggtatga agcaagttcg ccaagcagtg
     1021 acgctcaata tcatgggctt gcaacttgca cgtatgttgg gccccgctgc ggctggcttt
     1081 ttgctgaaag ccgttggttc tgccgcagcc ttctggatca atggcgcgag cttcctcgtg
     1141 gtcttgttta gtattttcat ggtcaagtct caccagcagg aaattcaccg ccgcgaaaac
     1201 ggacgtggac aattccgcga agtgttggat tacgtccgcg cacagccgcg catggtggat
     1261 ttattgatct tcgcctctct cgtgaccttt ttcggtctct ccatcatgaa cattctcccc
     1321 gccgtagccg atgacgtcct caaaggcgac tcgcaaacat ttggtctctt aatgggtgca
     1381 tcaggcgcgg gcgctttgat ctctaccttg ttcttattgc cactctccca gcaagccaaa
     1441 cggatcggtc gcattgtggg cggtgctgcg gtgtggatgg gcactttcta ccttttactt
     1501 tcccgttcca catatttgcc actttctatg ttgtctattt tcctcgttag tcttggagcc
     1561 ccattggtgc tgacgacggg attgggtgtg atgcaactca tggcgcccgg cgatatgcga
     1621 gcccgcatta ttagtctctt cacgatggtg tcttttggtt tacaaccgtt atcctctctg
     1681 attattgggt atgctgctga aacctttggt acacaaacca tcattatgat caatgctgca
     1741 tgcttaaccc ttggcggaat cctgatgctg atgctgagaa agggacttat ccaatgggaa
     1801 ctgaaaccca tggtagagcc gatcttatct tctcaagaaa aagcagcgat gcactaagcc
     1861 ttaatcaaaa aggactctca aacgagagtc ctttttgatt ctataaacct atagctggat
     1921 attttcacgg aaatttctgc cgaataacag aatgataata gctttcatga gaatagcaac
     1981 tttggcaaaa tatatgaccg ttgctatgga cttcacgctc attcatgatc tcttcaccgc
     2041 atacatcaca gttgacccgt accccgggac gcgagacgat ttcttggatg ggagttgtca
     2101 attccacctc tgtgatgttc aacatggttt catcaggcat agtctgatac gcctgcattt
     2161 gagcaaagta atgacgtggt tcgcttggca cgaataaagc ggcttgttcg cggatgtcca
     2221 atgcaggcgc aatgcgcaca gcgcgacccg tttttacatg cacaaacgtg acggcaactt
     2281 tgccgtaatc ttccacacgc aatgtgcggt gacctacggt gcaatctgtc gcggcaatca
     2341 caccatcgac aaaacaccca tctatttcag aaatgaccaa catctgttta tgttgcggtg
     2401 gttctgaaaa cccgagcgcc ttcatgccag ccaacccaat acgcacacct aaaatttgcc
     2461 ttgggcaaag gtgatcgtgg tctttcatag atttttc
//
LOCUS       DAOQJE010000577         2495 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_30502_length_2495_cov_2.243443, whole genome shotgun sequence.
ACCESSION   DAOQJE010000577 DAOQJE010000000
VERSION     DAOQJE010000577.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2495)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2495)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2495
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30502_length_2495_cov_2.243443"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1899)
                     /locus_tag="PLT08_13625"
     CDS             complement(<1..1899)
                     /locus_tag="PLT08_13625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE05560.1"
                     /translation="MNELIKLPEIFKGKWDHLLILTYGADLPYFENVILRSDFRCRNK
                     IILADGQKFLKACERYEKDGRVRSLNQRYIAEGIYLPNSAHAKIILLTTRESGKLLVG
                     SGNLGQGYTSGGELFTLYEYSTKDTSQLSAFQSVWKLVDTLLKRDMVGGAAVQSLRDL
                     YAGTPWLMQTTKNPDSMVVNNLHQDFIEQLQKKLDGENVQELWIMTPFFDAKCQALEK
                     LIDTFHPKAVNLVIQPKYVSLNPTALQRVGKKSKKKITIHPFETISQGQTAYVHAKLF
                     LLKTASRAICLQGSPNCSQVAMLLPSPLGNIEVANLLTGKRQAFDNLFKNLDLQPATT
                     NISDLEVAFQDIEPTPGVNKQDWLLTSATWRGDTMLLYFRGGAPDLQKAKLILGNFEF
                     NVTQVHIDNKEVQIKLPLDAQKVLSEHLVPIMIDTKSRRSSPIFPCHLEMLDREANTS
                     SFTISTQDIQNFNPEDPEFENLLRELEASFPIDRKSIWQLVGKSTSSRADDPDDDVYL
                     DYDKIDYDLIRQHPKIQQYLHAQGQGTSGQGLTRLQQLMSAITSHFAELSDVAFGKMK
                     VTSTFHLEGASNSDTEEEENGEVEQPLTPPKNRNRIRNALKRFINRYLRGLKSPDFVR
                     LVGAEVITN"
     gene            complement(1896..>2495)
                     /locus_tag="PLT08_13630"
     CDS             complement(1896..>2495)
                     /locus_tag="PLT08_13630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE05561.1"
                     /translation="PNSSFSDLLTWLSETVKAKPGKFDEMCKISSPINEHNLFVEAME
                     KSSPPFVMTAGLLTMLGLIVLRFRDAPLQRSPVWEIARMGAEIRLSMDSFIRNLDRFM
                     KVSHPTIVEVMRWIYENNVILQHELVATSKLPENTFRFQRDGDYLQFFNLSNSLGFMN
                     SRYDAISTTIYELGLCGDLHEPKHALTQDGRTLLQTGDL"
BASE COUNT          619 a          538 c          590 g          748 t
ORIGIN      
        1 gtttgtgatg acttctgcgc caacaaggcg cacaaagtcg ggacttttaa gaccacgaag
       61 atatcggttg ataaatcttt ttaatgcatt cctgatccga tttcgatttt tgggaggagt
      121 caatggctgt tcgacctccc cattttcctc ctcttctgta tctgagttac tagccccttc
      181 aagatgaaaa gtgctagtaa ctttcatctt tccaaaggct acgtctgaca attccgcaaa
      241 atgagacgta atcgcactca tcaattgttg cagacgtgtt aatccctgcc cgctggttcc
      301 ttgtccttgg gcatgaaggt attgttggat ttttggatgt tgccgaatga ggtcgtaatc
      361 aattttatcg taatccaaat agacatcatc atctggatcg tcggctcgcg aagaagtaga
      421 cttgcccacc agttgccaga tactctttcg atcaatcggg aacgatgctt ctaattctct
      481 tagtaaattt tcaaattcag ggtcttcggg attaaagttt tgaatatcct gggttgaaat
      541 agtgaaagat gaggtgtttg cctcgcgatc cagcatttct aggtggcagg gaaatatggg
      601 agaggatctc ctgcttttgg tatcaatcat gattggcaca agatgttcag ataatacttt
      661 ctgggcatcc aaggggagtt ttatttgaac ttccttgtta tctatatgta cctgggtaac
      721 attgaattca aaattaccca ggatcaattt cgccttttga agatccgggg ccccgccgcg
      781 aaaatagaga agcatcgtgt ctccacgcca tgttgcgctg gttagcagcc aatcctgctt
      841 gtttacaccg ggtgtcggct caatatcctg aaacgcaact tcaagatctg agatgttggt
      901 ggtggctggt tgtaaatcaa gattcttaaa gagattgtcg aaggcttgtc gttttccggt
      961 taatagattt gccacttcga tattgcctaa tggcgaggga agtagcattg ccacttggga
     1021 gcaattcgga gatccctgga ggcagatggc gcggctggcg gtttttagta aaaaaagctt
     1081 ggcatgaaca taggcagtct gcccttggga tattgtctcg aaaggatgga ttgttatttt
     1141 ctttttcgac ttcttgccaa cccgttgcaa tgcagttgga ttcaatgaga catactttgg
     1201 ctgaatgacc agattgacgg cttttggatg aaatgtatca atcaatttct caagcgcctg
     1261 acatttggcg tcaaaaaatg gcgtcatgat ccagagttcc tgaacatttt cgccgtcaag
     1321 ttttttttga agctgttcaa taaagtcttg atgaaggttg tttaccacca tgctgtctgg
     1381 atttttcgtg gtttgcatca accagggagt ccctgcatac aaatcccgca atgattgaac
     1441 agcagctcca ccgaccatat ccctttttag gagagtgtcc acgagtttcc aaacgctctg
     1501 gaacgccgac aattggcttg tgtctttcgt ggaatattcg taaagcgtaa atagttctcc
     1561 gccggatgta tatccttgcc caaggttgcc gctgccgaca agtagtttcc cggactcgcg
     1621 cgttgtaagc aggatgatct ttgcgtgggc gctgttgggt agataaattc cctctgctat
     1681 ataacgctgg ttaagtgatc gcacacgacc gtctttttcg tagcgctcgc aggctttcag
     1741 gaatttttgt ccatcggcaa gaatgatttt attgcgacac cggaaatcgg atcgcaggat
     1801 gacattttcg aaatatggca gatcagcccc ataggtaagg attaataaat gatcccattt
     1861 gcccttgaaa atctcgggga gtttgattaa ctcgttcata aatcccccgt ttgaagaaga
     1921 gttcttccat cctgggtgag tgcgtgtttg ggttcgtgta agtcaccgca cagtcccagt
     1981 tcgtagatgg tggtgctgat ggcatcgtat ctggagttca taaacccaag tgagttactc
     2041 aggttgaaga actgcagata atcgccatcg cgctggaatc tgaaggtgtt ttcaggtaat
     2101 ttgctggttg ccaccagctc atgctggaga attacgttat tttcataaat ccaacgcata
     2161 acttcaacaa ttgtggggtg tgagaccttc atgaacctat ccaggtttcg aatgaaagaa
     2221 tccatgctga gccgaatttc tgctcccatt cgtgcgattt cccaaaccgg cgaccgttgc
     2281 agtggcgcat cgcgaaaccg taaaacgatc aatccaagca tggtcagtaa gccagccgtc
     2341 atcacaaaag ggggactcga cttctccatc gcttctacga agagattatg ttcattgatt
     2401 ggtgatgaaa ttttgcacat ctcgtcgaat tttccgggtt ttgcttttac tgtttccgac
     2461 aaccatgtaa gcagatccga aaatgaactg tttgg
//
LOCUS       DAOQJE010000578         2490 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_30592_length_2490_cov_5.660370, whole genome shotgun sequence.
ACCESSION   DAOQJE010000578 DAOQJE010000000
VERSION     DAOQJE010000578.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2490)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2490)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2490
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30592_length_2490_cov_5.660370"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..452
                     /locus_tag="PLT08_13635"
     CDS             <1..452
                     /locus_tag="PLT08_13635"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0016018 - cyclosporin A binding [Evidence
                     IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HNE05562.1"
                     /translation="TIETAKGNIVIQLFPDKAPLAVNSFIFLAKQGWYDGVTFHRVIP
                     GFAAQAGDPSGSGRGNPGYFFNNEPNDLVFDKPGLVGMANSGPDTNGSQFFITFAPAE
                     HLNGAYTIFGQVISGLDVAEQLTARDPSQGGFLPAGDLILHITIEEK"
     gene            452..1720
                     /locus_tag="PLT08_13640"
     CDS             452..1720
                     /locus_tag="PLT08_13640"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070007 - glutamic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PrsW family glutamic-type intramembrane
                     protease"
                     /protein_id="HNE05563.1"
                     /translation="MQTKKIHWASLVILLILATSITFLLLIVLSLGVASLVDLFSSSA
                     EAASEMIGAVAFGFISILLLISGWFILQKTRGLEQADQNFKFPFAPWMWLVFPALVIF
                     SILFGGLITFAEIPWLNWLLLPFLTVLVIVPPIWLLFGIASNGLNLGPRWRFFSILGL
                     GMTAGPIIMIVLEMVLLLILIIAGVAYIAITRPEMVSEIESLANMLNQGLSEDAMLNL
                     LSPYLTNPTLLAIGIGYIALLVPLIEELFKPLAVWLFARQLGSPAQGFALGVLSGAAF
                     ALVESLNASADGTTSWAVIVAARTGTSVLHMLTTGLVGWGIAMAFKEKRFGRLVAAYL
                     SAVLIHGIWNAAAAGTGISALGESVGKPEWLFNYAPALVCGLLVLGIGTTSLLVASNR
                     KLRKQLELAPAVDNAVESTPVQETPETPSE"
     gene            1748..>2490
                     /locus_tag="PLT08_13645"
     CDS             1748..>2490
                     /locus_tag="PLT08_13645"
                     /inference="COORDINATES: protein motif:HMM:NF020287.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenine-N1)-methyltransferase"
                     /protein_id="HNE05564.1"
                     /translation="MKYSLNAREGDLAQLVGLTHKHFIFMLQSGGDFQSHRGVIKHDD
                     LIGKPWGSQIFSHTGAPFFLLQPSMSDILNELPRNTQILYPKDIGYILIQMGISEGQT
                     VLEAGTGSGSMTISMATAVGPNGRIVSYEKTPDTQNLAKKNLQRIGLASRVDFKLRDI
                     QEGFDETDADAFFLDVQNPFDYIQQVRATLKPGGFFGTLVPTYNQVEKVLYALKKHNF
                     AFVEVCELMLRYYKTNPARLRPTDRMVAHT"
BASE COUNT          573 a          631 c          614 g          672 t
ORIGIN      
        1 ccacgattga aactgccaaa ggaaatattg tgattcaatt atttccagat aaagccccgt
       61 tagcggttaa ttcatttatc tttcttgcga aacaaggctg gtacgacggt gtgacatttc
      121 accgcgtgat ccctggcttt gcggctcaag ccggtgaccc aagtggaagc ggtagaggca
      181 accccggcta tttcttcaac aatgagccga atgatcttgt gttcgataaa cctggtttgg
      241 tgggcatggc aaattctgga cccgatacca atggcagcca attctttatt acttttgcgc
      301 ccgctgaaca tttgaatggg gcttacacca tctttgggca agtgattagt ggattggatg
      361 tagccgaaca gttaacagcg cgcgaccctt cgcaaggtgg ctttttgccc gcaggggatt
      421 tgatcttaca tattacgatt gaagagaaat aatgcaaaca aaaaaaatcc attgggcatc
      481 tcttgttatc ttgcttatcc ttgcgactag catcacattt ttgcttttga ttgttctatc
      541 gctcggtgtc gcatccctcg tggacctatt cagcagttct gctgaagccg cttctgaaat
      601 gatcggtgca gtggcattcg ggtttatcag catcttgcta ctcatcagcg gatggttcat
      661 tttgcaaaag actcgcggat tggagcaggc agaccagaat ttcaaatttc cctttgcccc
      721 gtggatgtgg ctggtgttcc ccgccttggt gatctttagc attttattcg gcgggttgat
      781 cacctttgca gaaattccat ggctaaattg gctcctactt ccatttctaa ctgtcttggt
      841 cattgtccca ccgatctggc tgttgttcgg catcgcctcg aacgggctga acctcggtcc
      901 gcgttggcgt tttttcagta ttctcgggct aggcatgacg gcgggtccga tcattatgat
      961 cgtgctcgaa atggtacttt tgctaatcct catcattgcc ggggttgcct atattgccat
     1021 cacacgccct gagatggtaa gcgaaattga gtctttggca aacatgctaa accaaggttt
     1081 aagcgaagat gccatgctca atctgttatc gccttacctt accaacccaa ctttgctcgc
     1141 cattgggatc gggtatatcg cgcttctggt tccgctgatt gaagaattat tcaagccgtt
     1201 ggcagtttgg ctgtttgccc gtcaacttgg atctccggcg caaggatttg ctctgggtgt
     1261 actcagcggt gcggcgtttg ctttagtaga aagcttgaat gccagtgcgg atggcaccac
     1321 tagttgggcg gtcattgtag cagcacgcac tgggacgagt gtactgcaca tgctcaccac
     1381 gggcttggtt ggctggggaa ttgcgatggc gttcaaagaa aaacgctttg gacgtttggt
     1441 cgccgcatat ctctctgctg tgttgatcca cggcatttgg aatgccgctg ccgccggcac
     1501 aggcatttct gccttgggtg aatcggtcgg caaacctgaa tggcttttca attatgcgcc
     1561 tgcgctcgtt tgcggcttac tggtgttggg cattggcaca acttctttgc tggttgcctc
     1621 caaccggaaa ttaagaaaac agctcgaact cgctcccgct gtggataacg ctgttgaatc
     1681 cacgccggtt caggaaaccc cagaaacccc ttctgaataa atcgaaagta aaggtagaat
     1741 tcgctgcatg aaatattctc ttaacgcgcg tgagggcgac ctcgcgcaat tggtggggct
     1801 aacccacaaa cacttcatct ttatgctgca atcaggtggc gactttcaaa gccaccgtgg
     1861 tgtgatcaag catgatgatc taattggcaa accgtggggc tcgcagatct tcagccatac
     1921 aggagcgccc ttcttcctgc ttcagccttc catgtccgac attctcaatg aattgccgcg
     1981 caatacgcaa attctgtacc ccaaagacat cggctacatc ctcatccaaa tgggcatctc
     2041 tgaagggcaa accgtactcg aagctggcac aggttctggc tcgatgacga tatccatggc
     2101 aacagcagtt ggtccaaacg gaagaatcgt ttcgtatgag aaaacgccag atacgcaaaa
     2161 cctggcgaag aaaaatctac aacggatcgg tctcgcctct cgcgtggact tcaagctgcg
     2221 tgacatccaa gagggcttcg acgaaacgga tgccgatgcc ttcttcctcg acgtgcaaaa
     2281 cccgttcgac tacattcaac aggtccgcgc cacgctcaag ccgggcggat tctttggaac
     2341 gctcgttcct acctataatc aggtcgaaaa agtattgtat gccctgaaaa aacataactt
     2401 cgcttttgtg gaagtctgcg aattgatgct gcgctattac aagaccaacc ctgcccgctt
     2461 acgtcccaca gaccgtatgg tggcgcacac
//
LOCUS       DAOQJE010000579         2490 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_30593_length_2490_cov_5.627926, whole genome shotgun sequence.
ACCESSION   DAOQJE010000579 DAOQJE010000000
VERSION     DAOQJE010000579.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2490)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2490)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2490
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30593_length_2490_cov_5.627926"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2090
                     /locus_tag="PLT08_13650"
     CDS             <1..2090
                     /locus_tag="PLT08_13650"
                     /inference="COORDINATES: protein motif:HMM:NF012234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNE05565.1"
                     /translation="YGGIGWVIGLLLTLFVSAPVIWFLTQNASRADRADILAYGLAAA
                     WWLNPLSLGGLFYVFSNIRMWRFWTLPALFVWLLINAMVSASIASDVAREWVLNALFI
                     GGLVILGLLFLYIQLRVFVWAASGNASRFFFGIIGWALLNGLVVWALVNPDVLNIVLV
                     ALGFIFRVVFAIGFAIIQFVAIFWFMSQTRVEIIRPGDPKQITFDDYKGQPNLLKMVR
                     QWIYLLSDREQFQKMGGQFITGLLLYGEPGTGKTLLAKAMAGEAGIAFISIEGSGFRG
                     MFWGVDVLRMVQFVGRARKLAREYGACIAYIDEIDAVGMSRGSVMGGGQAMGGMMGMG
                     AGSGALTRLLYEMDGIGTLTPWEKLRARVYQMLRRKAPVRNWHVMFMGSTNRPDVLDP
                     ALVRPGRFDRKIAVGLPDRGGRREIVKYYLSKIKHDETVDIEALVSDTSWATPARIMS
                     AVTKDAVRLALFDGRDKISQRDIELAFQEQAIGLENPIEEMEEDQRRQVAYHEAGHAV
                     AQYHLRPDERIVRVSIIRRADALGYVLPVSNFDVYAMPLRRFVADIMVSMAGHVATKV
                     FFGEYWTGATGDFQNVRARLWQLAHYGYFGPPITMDSAMGAEGGYNGRGKIVEKFWQQ
                     LEDQTEQLLRTHRIEVEAVAQALLERGDLTGRECVEIIRQASGEGQADKTETDKMLSA
                     LVSDVTGEKPEEK"
     gene            complement(2137..>2490)
                     /locus_tag="PLT08_13655"
     CDS             complement(2137..>2490)
                     /locus_tag="PLT08_13655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE05566.1"
                     /translation="AFFWSPDAIEVAYFIPTVTQAEGTTDPLVYLDLYILDIASKESR
                     KIATFQPTEGFLSMLPYIDQYHQSTTIWSPDSNNIVISFIDPNGNSGIAIIPSSGVTE
                     PRLLVDGLYASWSWK"
BASE COUNT          529 a          559 c          712 g          690 t
ORIGIN      
        1 tttatggggg aattggatgg gtcattgggc ttttgctcac tcttttcgtt tcagctccag
       61 tcatttggtt tttgacgcag aacgcctcgc gcgccgaccg ggcagatatc ctcgcctatg
      121 gcttggcagc cgcctggtgg cttaatccgc tatcgctcgg cgggttgttc tatgttttta
      181 gcaacatccg tatgtggcgc ttttggacgc tccccgctct gttcgtttgg ctattgatca
      241 atgccatggt gtctgcgtcc attgcttctg atgtggcgcg tgaatgggtc ttgaacgccc
      301 ttttcattgg cggtttagtc attctaggtt tgctgttttt atacattcaa ttacgcgtat
      361 ttgtttgggc ggcatctggc aatgccagcc gctttttctt cggcattatt ggttgggctt
      421 tacttaatgg cttggttgtt tgggcattgg ttaaccctga tgtgctgaat attgttcttg
      481 ttgccttggg ctttattttc cgcgttgtat ttgccattgg cttcgccatc atccaatttg
      541 tggcgatctt ctggttcatg tctcaaacac gtgtggagat cattcgcccc ggagacccga
      601 agcagattac ctttgacgat tacaaaggtc agccgaacct gctcaagatg gtccgccagt
      661 ggatttatct attgtctgac cgtgaacaat tccaaaaaat gggtggacaa ttcatcaccg
      721 gcttgttgct ctatggtgaa cctggtacgg gtaaaacctt gctagccaaa gccatggcag
      781 gggaagcggg tatcgctttt atctccattg aaggctctgg tttccgtggc atgttctggg
      841 gcgtggatgt gctccgcatg gttcaattcg ttgggcgtgc tcgtaagctt gcacgtgaat
      901 atggcgcttg tatcgcctat atcgatgaaa ttgatgctgt gggtatgtct cgtggaagtg
      961 tgatgggcgg cgggcaggcg atgggtggca tgatgggtat gggcgccggc tctggcgcgt
     1021 tgacccgcct cctctacgaa atggacggga ttggtacgtt gacaccgtgg gagaaactgc
     1081 gtgcgcgtgt ctatcaaatg ttgcgccgta aagcacctgt ccgcaattgg catgtgatgt
     1141 tcatgggctc taccaaccgc cccgatgtgc ttgacccggc attggtgcgc cccggtcgtt
     1201 ttgaccgcaa gattgctgta ggtctgccag accgtggtgg tcgccgtgag atcgtgaagt
     1261 actacttgag caagatcaaa cacgatgaaa ctgtagacat tgaagcgttg gtcagtgaca
     1321 cgtcttgggc aactcccgct cgtatcatgt ctgcagtgac aaaggatgcg gtccgtttgg
     1381 cattgttcga cgggcgcgat aaaatctctc agcgtgatat tgaattagcc ttccaggaac
     1441 aagcgattgg tcttgaaaac cccattgaag aaatggaaga agaccagcgt aggcaagtgg
     1501 cataccatga agctgggcat gctgtagcgc aatatcactt gcgccccgat gaacgcattg
     1561 ttcgtgtgag cattatccgc cgtgccgatg cgttaggata tgtgttgccc gtctcaaatt
     1621 ttgacgtgta tgccatgccg ctgcgacgct ttgtagccga tattatggtc tctatggctg
     1681 ggcatgtggc tacgaaagtc ttctttggcg aatattggac cggtgccaca ggcgacttcc
     1741 aaaacgtccg cgcaaggttg tggcagttgg cacattatgg ttattttggt ccgccaatta
     1801 ctatggacag tgccatgggc gcagagggcg gttataacgg gcgtggcaag attgtggaga
     1861 agttctggca gcaattggaa gaccagaccg agcaactctt gcgcacccat cgcattgaag
     1921 tggaagccgt tgcccaagct ttgctcgagc gcggtgacct gaccgggcgc gaatgtgtgg
     1981 agattattcg ccaggcttcc ggcgaaggtc aggctgacaa gactgagaca gataaaatgc
     2041 tcagtgcact ggtcagtgat gtgacgggtg aaaagcccga agaaaagtaa acagtaacaa
     2101 aaacgccctc gattttcgag ggcgtttttg tttaacctat ttccaagacc atgaagcgta
     2161 taacccatcc acaagaaggc gcggctcggt aacgcctgat gatggaatga tggcaatgcc
     2221 tgaattgcca ttcgggtcta tgaacgagat gacgatgttg ttactatctg gcgaccagat
     2281 cgttgtggat tgatgatact ggtcgatgta aggaagcatc gagagaaaac cttcagtagg
     2341 ttggaaggtg gcgattttgc ggctctcttt gctggcaata tccagaatat ataggtcgag
     2401 ataaaccaat gggtctgttg tgccttctgc ttgtgttacc gttggaatga aataggcaac
     2461 ctcaatcgca tctggtgacc agaagaaggc
//
LOCUS       DAOQJE010000580         2486 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_30692_length_2486_cov_5.572604, whole genome shotgun sequence.
ACCESSION   DAOQJE010000580 DAOQJE010000000
VERSION     DAOQJE010000580.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2486)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2486)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2486
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30692_length_2486_cov_5.572604"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2486)
                     /locus_tag="PLT08_13660"
     CDS             complement(<1..>2486)
                     /locus_tag="PLT08_13660"
                     /inference="COORDINATES: protein motif:HMM:NF025056.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIR domain-containing protein"
                     /protein_id="HNE05567.1"
                     /translation="EVFVFLISPDSIKSEVCKVELEHARKNSKRIIPVVARDVVVQDV
                     TPILRDLNWIFIREQDDFAAGLEKVNLAINLDIEWLQEHRRLQVRALEWDRKKESSLL
                     LRGTDLRNARKMIVANEKKDPFPSPLQKLYIESSRRGEQLRLTTYVSAAVTLFIMVFL
                     SLVALNQRQEAISQRQVAQENEKIAQEARKEADRNASIARAQKDKALANEKIARAQRS
                     AARAQIYQSRTGGLFTSTLLGIDSYQRNPSVEAEEILRKNISLLPIPVKQIEQNGAIL
                     HIEVSPQGTSFVSTSEDGTACLINFESGESTFCVQSSGAVLDAAFSPDGSKLVISDAT
                     GEVQILNAETGEILKQIDLGVSVRDVNISPDGTLLAMARDDARISLIKLSNYGFAGEF
                     SVYGSLRVTTFSPDGEWFAAASNLGAITFWNLGTGEIVNGGAHRGEVFEIAFSPNSRE
                     LISGAADNCAVLTSPSSGKQILKVLNEDRVTNVAFSPDGSWFVTSSDDFRIRVWDSAS
                     GAERFRFLQDSVVKEVKVSPDAMWIASTGSDRTARVWSAANGAEMYQIPLDAEGRVLG
                     FSSDTNYLVVGDAEGNLSIWDISSLAMNDGYMRFDEYVSDIAMSPNGDWLAAADKGQV
                     WLLDPELLPSQIVPTGKLLYTFNSDTVTDMAVNPKGDSFAVSTQGGQVVFITSQGVTR
                     VVTSSGAQQALAFSTDGSTLFLAGNNGQLQSRSITSGENASVWQGATSIYSIAVSPSE
                     QISIGMDDKIVLLQVGSTTSEIDLPAPGKNTQVVFNADATLLASSTPDGRAYVWQLKN
                     GEYKRIVELTSAPISSIAFSPNGDFLFLGET"
BASE COUNT          703 a          631 c          511 g          641 t
ORIGIN      
        1 gtttccccaa ggaagagaaa atctccattg ggtgaaaatg ctatcgaaga aataggagcg
       61 ctcgtaagtt caacgatccg tttgtattca ccatttttca attgccagac atatgcccga
      121 ccatcaggcg tactggatgc caacaaggta gcatcagcat taaaaacaac ctgcgtattt
      181 ttacctggag caggcaggtc aatttcagag gtagttgaac ctacttgtag caagacaatc
      241 ttatcgtcca tcccaattga aatttgttca cttggagaga cagctattga gtaaattgaa
      301 gtagctccct gccagacaga agcattctca ccagaggtaa tacttcgtga ttgtaactgt
      361 ccattattgc ctgcaaggaa taatgtgctt ccgtctgttg agaaagcaag agcctgctgc
      421 gcaccagaag atgtgactac acgtgttaca ccttgtgatg taataaacac aacctgccca
      481 ccttgagtgg acactgcaaa tgagtcacct ttggggttca ctgccatatc ggtgacggta
      541 tctgaattaa aagtgtacag taatttgcca gtaggaacga tctgagatgg gagcaattcc
      601 gggtccagca accagacctg ccctttgtct gcggcggcaa gccaatcgcc attggggctc
      661 atagcaatat cactgacata ctcatcaaag cgcatatacc catcgttcat ggcaagcgaa
      721 gaaatatccc aaatagatag gttgccttca gcatcaccaa cgacaagata atttgtatca
      781 gagctaaaac ctagaaccct gccttcagca tctaatggaa tctggtacat ctctgcccca
      841 tttgcagcac tccacacacg tgccgtccta tctgaaccag tagaagcaat ccacatggca
      901 tcggggctga ctttgacttc tttcacaaca ctatcttgca agaagcggaa acgctcagca
      961 ccagaggcag agtcccaaac gcggatgcgg aaatcatctg atgaagtgac aaaccaggag
     1021 ccgtcagggc taaatgccac attggtaaca cgatcttcat tcaatacttt taatatctgt
     1081 ttccctgaag aagggctggt aagaacagca cagttatcgg ctgctccaga gatcaactca
     1141 cgactatttg ggctaaaagc gatttcaaaa acttcgccac gatgcgcacc gccgttgaca
     1201 atttctccag tgcccaaatt ccaaaatgtg atcgcaccca ggttcgaagc agctgcaaac
     1261 cattcaccat ctgggctaaa tgtcgtaact ctcaaactgc catataccga gaattctccc
     1321 gcgaacccat aattggaaag ttttatcaaa gaaatgcggg catcatcgcg tgccatggca
     1381 agcaaagtcc catctgggct gatgtttaca tcacgcaccg aaacgccaag atctatctgt
     1441 ttaagaatct cacccgtttc agcatttagg atctgcactt cacccgttgc atcactaata
     1501 accaatttgc taccatctgg actaaacgcc gcatctaaca cagccccaga gctctgtaca
     1561 cagaatgtgc tttctccact ctcaaagttg atcaaacaag ctgtaccatc ttcgctggta
     1621 gaaacaaacg atgtaccttg cgggctgacc tcaatgtgca agatggctcc gttctgctct
     1681 atctgtttga cagggatcgg cagcaagcta atattctttc gaagaatttc ctctgcctca
     1741 acagatggat ttctttgata tgaatctata ccgagcaaag tgctagtaaa cagtccacca
     1801 gttcgagact gataaatctg cgctcgtgca gcactacgct gtgctctggc aatcttctca
     1861 ttggcaagcg ccttgtcttt ctgagcacgc gcaatagacg catttcgatc cgcttcttta
     1921 cgcgcctctt gcgcaatctt ttcattttct tgcgccactt ggcgttgact gatcgcttct
     1981 tgcctttgat tcaatgccac aagggaaaga aacaccataa taaacaaagt cactgcggca
     2041 gaaacgtatg ttgtaagacg gagttgttcc ccacgccggc tggattcaat atatagcttt
     2101 tgcaaaggcg aagggaaagg gtctttcttc tcattcgcca cgatcatttt tctcgcatta
     2161 cgcaggtcag taccgcgtag taacaagctg gattcttttt tacgatccca ttccaaggcg
     2221 cggacttgca aacgtctatg ttcctgcaac cattcaatat ctaggttgat cgccagattt
     2281 actttttcaa gcccagcagc aaaatcatct tgctcacgga taaaaatcca gttgaggtct
     2341 cgcaatatgg gggtcacatc ctgcacaacc acatcacgag ctactacagg gatgatacgt
     2401 tttgaatttt tacgagcatg ttccaattcg accttacaga cttctgattt gatcgaatca
     2461 ggactgatga gaaatacaaa aacttc
//
LOCUS       DAOQJE010000581         2482 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_30777_length_2482_cov_6.711166, whole genome shotgun sequence.
ACCESSION   DAOQJE010000581 DAOQJE010000000
VERSION     DAOQJE010000581.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2482)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2482)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2482
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30777_length_2482_cov_6.711166"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..429
                     /locus_tag="PLT08_13665"
     CDS             <1..429
                     /locus_tag="PLT08_13665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE05568.1"
                     /translation="TFMRYMWDDRFEAPVIKPLYPPLAEGEVPSYDVHIQPIVKRYCV
                     SCHRAGKDNNNFLMTSYEEILTTGDQSANNVIAGDENSYLLRVIQAHPILDENGNEVI
                     GVMPPTKALKPDIVDVFIRWVMNGMPQTAEDAAALSTPAP"
     gene            564..899
                     /locus_tag="PLT08_13670"
     CDS             564..899
                     /locus_tag="PLT08_13670"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015742582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HNE05569.1"
                     /translation="MKTDSGLEYIETQAGTGTQATAGKTVFVHYTGKFQNGQVFDSSI
                     PRGEPLAFKLGVGQVIKGWDEGIALMKVGGKAQLIIPSNLAYGERGAGGVIPPNATLV
                     FDVELVDVK"
     gene            1058..1510
                     /locus_tag="PLT08_13675"
     CDS             1058..1510
                     /locus_tag="PLT08_13675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE05570.1"
                     /translation="MSFQEKNIVASLSTFTLILFVYMFRVFQMVQTKSFTAPNVFWLW
                     GIVAILAIAGTILLSILTHIAGGIVHKIRTDEDPHIEDIQDERDQLIDLKGTRVAYTV
                     SSLGVAASMLTFVFGQPPLVMFSALIFFGVLAQIVADGWRLNLYRKGF"
     gene            1512..1739
                     /locus_tag="PLT08_13680"
     CDS             1512..1739
                     /locus_tag="PLT08_13680"
                     /inference="COORDINATES: protein motif:HMM:NF024252.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HNE05571.1"
                     /translation="MEKGRIGNNIRKLRFFRDEMTQQQLAEKVGVTRQTIIAMEQDKY
                     SPSLELAFRIAHVFGVPLEEVFTYQPEDSKE"
     gene            1772..1939
                     /locus_tag="PLT08_13685"
     CDS             1772..1939
                     /locus_tag="PLT08_13685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE05572.1"
                     /translation="MKTKNQKTSGILFIFAGLAFIVAYWLSKQVSFIGVGIAFIGIGA
                     SFIAKSNAVEK"
     gene            1953..2432
                     /locus_tag="PLT08_13690"
     CDS             1953..2432
                     /locus_tag="PLT08_13690"
                     /inference="COORDINATES: protein motif:HMM:NF025687.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4386 domain-containing protein"
                     /protein_id="HNE05573.1"
                     /translation="MTSTTPYRKTEIYVALLWIITAAGAIGGASLINPIINAPDYLTL
                     VFPKSVTLTSGMFGWMINDIGIVFIGLLMYPILKKHSESMALGYLSMRMFESLLMIVG
                     VFFALMLIPLSRDFINAGTADVSTLQTIGSILKQAENWFLNLLQLIFLGIGGLININ"
BASE COUNT          678 a          662 c          549 g          593 t
ORIGIN      
        1 acctttatgc gttacatgtg ggatgaccgc ttcgaagcgc ctgtcatcaa accgctctac
       61 ccgccgctcg cagaaggcga agtgccatcg tacgacgttc acattcaacc cattgtgaag
      121 cgctattgtg tctcctgtca ccgcgcgggc aaagataaca ataacttcct gatgaccagc
      181 tacgaagaga tcctcacgac tggcgaccaa tcagccaaca acgtcatcgc aggggatgag
      241 aattcctacc tcctgcgggt tatccaagcc catcccatcc tcgatgaaaa tggaaatgaa
      301 gtcatcggag tcatgccgcc cacaaaagcg ctcaaacccg atatcgttga cgttttcatc
      361 cgttgggtca tgaacggcat gccacaaact gcggaagacg cagccgcact ctccacacca
      421 gcaccttaat gcctatacct taattcgtaa aagccgggac agttgtctcg gctttttgaa
      481 tcccctttcc taagttggga gaagggtagg gggtgagggt aagttacagg tacaataata
      541 aaaattcaaa aaatggagaa cccatgaaaa cagatagcgg actcgaatac attgaaacac
      601 aagccggaac cggcacccaa gccacagccg gcaaaaccgt gtttgtgcat tacaccggca
      661 aattccagaa cggacaagtc ttcgacagct ccatcccacg cggtgaaccg ctcgccttca
      721 aactcggagt cgggcaggtc atcaaaggct gggacgaagg catcgccctg atgaaagtcg
      781 gtggtaaagc ccaactcatc atcccttcga acctcgccta cggcgaacgc ggcgccggcg
      841 gagtcatccc ccccaacgcc accctcgtct tcgatgttga actggtagat gtgaagtaat
      901 tcataaaatt ccgctcatgc ttttgaaatg cgaccctttt cgggtcgcat ttttgtttcc
      961 tcatagtcca aattgcaatg taagaaatac ttgacattta gtaaaataca ggttactata
     1021 agtaatataa agcagacaat caaaaagtga ggcaaaaatg tcatttcaag agaaaaatat
     1081 cgtcgcttcg ttatccacct tcacactcat tctgttcgta tatatgttca gggtctttca
     1141 aatggtccaa accaaaagtt tcaccgcgcc caatgtattt tggctatggg gcatcgttgc
     1201 gatccttgcc attgccggaa ccattcttct cagcatcctc acccacattg ccggagggat
     1261 cgtccataag attcgcacgg acgaagaccc tcacattgaa gacattcagg atgaacgcga
     1321 ccaactcatc gacctaaaag ggacgcgtgt ggcatacacc gtttcttcgc tcggggtcgc
     1381 cgcctccatg ctgacctttg tctttggtca gccacccctg gtcatgttca gcgccttgat
     1441 ctttttcggc gtcctcgcgc agatcgtcgc agatggttgg cggctcaacc tgtaccgaaa
     1501 gggcttctag catggaaaag ggcagaatcg gcaacaacat tcgcaaactg cgcttcttcc
     1561 gagatgagat gacccaacaa caactcgcgg aaaaagtcgg tgtcacccgt cagaccatca
     1621 tcgccatgga gcaagataaa tactcgccct ctctagagtt ggcatttcga atcgcccatg
     1681 tcttcggagt gcccttggaa gaagtcttta cctatcaacc cgaagactct aaagaatgaa
     1741 ccatacatct taaaaaagga atcaaataaa catgaaaacg aaaaatcaaa aaacgagcgg
     1801 aatcttattc atcttcgctg ggctcgcctt tattgtggcg tactggctca gcaagcaagt
     1861 ttcgtttatc ggggttggca tcgccttcat tggcatcgga gcctcgttca ttgccaaatc
     1921 caacgcagtc gaaaagtaag ggagtttctt aaatgacttc aactactcca taccgtaaaa
     1981 cggaaatcta cgtagcgtta ttatggatca tcaccgccgc cggagcgatt ggcggcgcaa
     2041 gcctgatcaa ccccatcatc aacgcacctg actatctgac cttagtcttc ccgaaaagtg
     2101 tcaccctcac aagcggcatg ttcggctgga tgatcaacga catcggcatc gtcttcatcg
     2161 gtctgttgat gtacccaata ttgaagaaac acagtgaaag catggcgctc ggatatttaa
     2221 gcatgcgcat gttcgaatct ttgttgatga tcgtcggcgt cttcttcgcg ttgatgctca
     2281 tccccttaag ccgagatttc atcaacgccg gcacagccga tgtttctaca ctgcaaacca
     2341 tcggttccat actcaaacaa gcggaaaatt ggttcttgaa cctcttacaa ttgatatttc
     2401 tcggcattgg cgggctgata aatatcaatt gaaagaggtt caagaaccaa ttttccgctt
     2461 gtttgagtat ggaaccgatg gt
//
LOCUS       DAOQJE010000582         2479 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_30861_length_2479_cov_3.183168, whole genome shotgun sequence.
ACCESSION   DAOQJE010000582 DAOQJE010000000
VERSION     DAOQJE010000582.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2479)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2479)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2479
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30861_length_2479_cov_3.183168"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            198..2342
                     /locus_tag="PLT08_13695"
     CDS             198..2342
                     /locus_tag="PLT08_13695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE05574.1"
                     /translation="MSFFITFKKLPWAIAFRVLTLTVILLVNFLNLLWLPNQIHRLRH
                     GGIEGLTLEFTRDSGNVTVNSAIIERMNLSGEATVTFADRDANKSGIYAGSIVLNSEA
                     LTSGPIGTTVTLFVKSGGDKREVNLTRRQYNNPLNILSFFSRISPLVQMYIEIIILCA
                     AILLTMLFSIVAFWYQSDEWLAFFTTGAIMNFFAPIQQSPPSYLLVSYFITGAILFLI
                     LFPKGKVKPKWSWLLVLLPVPHLIKIIYQVYVYEFPLFIDDKLIYFLESAMPTLVLII
                     FGAITYWCRNSLTLIERKRINWLILGLFLVFTFRFIWTLPSVARLIPDQGANYTQYNL
                     NLYKLIIFRLNRTIYAIYRSLTSLETASVLIIFGYLIYKYIHLVSPLEKQQMKWMALG
                     LIVGEIPIIVAYMFLAFFFNRLQIDWEHASLMLSDTNRGDYLNLVTAGIDKLLLVEMF
                     LIGLSIVAAKKHYRLWDVDTFINHALVKGGLTILAGLLITITATFIDQTQGRAAFNQN
                     ALLFIPISILLIAATYKPSKAWLQNRADNFIPLEKINFKETYLEFTTDVHEFFTQREL
                     SKILVEKSIKQMKVTHASVFLKNKNGKLQHIKTASTRKKVIKPVIHTQTHSKLRMGEV
                     VTSDASFGHSIIIPLLVPRGQNSDLIGALLLGPRITETGYSSEMKDCLRKLGEEIGTS
                     FYLSQVKRQRQRLKTPRQTHPKSASHKKSTQR"
BASE COUNT          765 a          554 c          465 g          695 t
ORIGIN      
        1 caattcagca aacgctttga acgcctcgaa aaagcccgcg gcgcaagtct ggatgaaata
       61 aatcgcgtgg cgcgcgaagc ggaagaagcg taagactaaa accatcaccc agcaaaaaca
      121 ccagttcagg aagagtttcc agataaatcg tataatggtt attcaaacat gaacattcac
      181 caagaggcgg aggtcaaatg tcttttttca tcactttcaa aaaactacca tgggcaatag
      241 cgtttcgcgt ccttacattg acagtcatat tacttgtaaa ttttttgaac cttttatggc
      301 ttccaaatca aatccacaga ttaaggcatg gcggaataga agggttaaca ctggaattca
      361 caagggattc gggcaatgta acggttaaca gcgcaataat tgagcgcatg aatttatctg
      421 gggaagcaac agtcacattc gctgataggg atgccaacaa aagtggaata tatgcagggt
      481 ctatagtgct aaactcagaa gcgctaacca gcggaccaat cggcaccacg gtcactcttt
      541 ttgtgaaaag tggcggagat aaacgggaag taaaccttac ccgcagacaa tacaacaacc
      601 cactcaacat tctttctttt ttctccagga tatccccact tgttcaaatg tatatcgaaa
      661 tcataatact ctgcgcagca attcttctaa ccatgttgtt tagtattgtg gctttttggt
      721 atcagtctga tgaatggctg gcttttttta ctactggagc aatcatgaat ttttttgccc
      781 ccatccaaca aagccctcca tcctatttgc tggtgtcgta tttcataacg ggggcaatcc
      841 tgttcttaat tctttttccc aaagggaaag taaagccaaa atggtcatgg ttgcttgttt
      901 tgctgccagt tccacatctt attaaaatta tatatcaggt ttacgtatac gagttcccgc
      961 tttttattga cgacaaacta atttatttcc tcgaatctgc catgcccaca ctggttctga
     1021 taatctttgg agcgatcaca tattggtgtc gtaattcgtt gaccctgatt gaacgaaaaa
     1081 gaattaactg gctcattttg gggctgtttt tggtgtttac atttcgcttc atatggacac
     1141 ttccgtcagt tgcgcgatta atacccgacc agggcgccaa ttacacacaa tacaatctta
     1201 acctttataa gctcatcatc tttagattaa atagaactat ttacgccata tatagatcac
     1261 ttacctctct tgaaacagcc tcggttttaa taattttcgg ctatttaata tacaaataca
     1321 ttcacctggt ttctcctctt gaaaaacaac aaatgaaatg gatggcgctt ggattgattg
     1381 tgggtgaaat accaatcatc gtagcttata tgtttcttgc attttttttc aaccgcctgc
     1441 aaatcgactg ggagcacgct tcgctcatgc tcagcgatac taaccgtgga gattatctga
     1501 accttgttac agcaggaatc gacaaacttt tgctggttga gatgttctta ataggtttat
     1561 ccattgtagc tgccaagaaa cactaccggt tgtgggatgt agatactttt atcaaccacg
     1621 ccctggtaaa agggggttta acgatattgg caggtttgct cattaccata acagcaacat
     1681 ttatagatca aactcagggc agggccgcct tcaatcaaaa cgcccttctt tttattccca
     1741 tttcaatact tctcatcgct gccacttaca aaccatctaa agcctggctg caaaatcgcg
     1801 ccgacaactt cattccactt gaaaaaataa acttcaagga aacttatctg gaattcacca
     1861 ctgatgtgca tgaatttttc actcagcgtg aactctcaaa aatacttgta gaaaaatcga
     1921 tcaagcaaat gaaagtcact catgcatctg tttttctaaa aaacaagaat ggaaaacttc
     1981 aacacatcaa aacagcctcg accagaaaaa aagtaatcaa acctgtcatc catacgcaaa
     2041 cccattcaaa actgaggatg ggcgaggtcg tcacctctga tgcgagtttt ggtcactcga
     2101 tcattattcc attactggtg ccacgcgggc aaaactctga cttaataggt gcgctattat
     2161 tagggcctcg catcactgaa acagggtatt cctctgaaat gaaagactgt ttgaggaaat
     2221 taggcgagga aataggaaca tccttttatt tatcacaggt aaaacggcaa aggcagagat
     2281 tgaaaacgcc tcgtcaaaca catccaaaat ctgcatctca taaaaagtcc actcaacgct
     2341 gaagtcaatg ggtgaggata aagatgagat aaaatccacc gaagcaatat cgaccctccg
     2401 ttaagaaaga aacaaaatat ccagtgacca aacgcatttt catctccgcc gatcacggca
     2461 tggcaatcat ctacttcct
//
LOCUS       DAOQJE010000583         2475 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_30949_length_2475_cov_5.065289, whole genome shotgun sequence.
ACCESSION   DAOQJE010000583 DAOQJE010000000
VERSION     DAOQJE010000583.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2475)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2475)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2475
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30949_length_2475_cov_5.065289"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..550
                     /locus_tag="PLT08_13700"
     CDS             <1..550
                     /locus_tag="PLT08_13700"
                     /inference="COORDINATES: protein motif:HMM:NF013696.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HNE05575.1"
                     /translation="AEVEKVKLGEHPLMGPAILALTDIISEPYPGYSVIPALCKVTYD
                     RRLLPGETPEGVLNEITSLPALKDINFKAKIAQSDHVTYTGETLSANKFFPAWELDEE
                     HEFVQKALKGLRASGLDPKLGAYRFCTNAAYSIGTAGVPTIGFGPGAEGDAHVVDEQL
                     SLDELAKTARGYVGIIEAVLGV"
     gene            570..1439
                     /locus_tag="PLT08_13705"
     CDS             570..1439
                     /locus_tag="PLT08_13705"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HNE05576.1"
                     /translation="MSTTLLSILLGIGGMLGWGIYDFLGGVFSKQIGSFKSLFWSQLA
                     GLISILLLAFAFKTTFEIPILALALSPLASIVYSAGYLFFFKGFEKGNVSIIAATMNL
                     WAVFTMIFAFLFMGQRLSITQTVGVFMILSGATLAAIDWNSIKNQGFQLSLGVREAVL
                     GAFFFGVFWNISEIVSEKIGWLLSTVLTKLGITIFLLVFSLFAKQETGLTNIPTKTKT
                     IVSLMGIIEVSAVALVNYGLTIGDAILITPIASALSIVTIALAVIFLKDKLSKLQLIG
                     VATAILGIIATAF"
     gene            1455..2078
                     /locus_tag="PLT08_13710"
     CDS             1455..2078
                     /locus_tag="PLT08_13710"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HNE05577.1"
                     /translation="MALVIFDYDGVLADTLDDLIHFGQEACNQLGVNHVVTKDDLSNL
                     EVMSFATYGRACEVPEPLIDEFVKISLDLFAKKASPPAIFNGVREVIQHFSNKHKIAI
                     VTTNSSQNVHAFLTQHGLDSLVHAVYGVDTPGSKAQKISIARERFANNGEAIFMIGDA
                     LSDIRSAKEAGVTSIAVTWGHQSLETLLRGSPDFVINFPHELIKIIE"
BASE COUNT          667 a          565 c          555 g          688 t
ORIGIN      
        1 tgccgaagtt gaaaaggtta aacttggcga acatccgttg atgggtcccg ctatccttgc
       61 attgaccgat attatctcgg agccataccc tggttactcc gtcatccccg cgctttgcaa
      121 agtgacctat gatcgccgcc tcctgccggg tgaaacgccc gaaggtgtgt tgaatgaaat
      181 cacttcgtta cctgcattga aagatatcaa cttcaaggcg aagatcgcgc aaagcgacca
      241 cgtcacctac accggcgaaa cgttgagcgc aaataaattc ttccccgcct gggagttgga
      301 tgaagaacat gaatttgtac agaaagcatt gaaaggatta cgcgcctctg ggcttgaccc
      361 caaactgggt gcctaccgat tctgtaccaa cgccgcatac agtatcggca cagccggtgt
      421 gcccaccatt ggctttggtc ccggtgcgga gggagatgct cacgtggttg atgaacaact
      481 cagcctcgat gaactcgcca agacggctcg agggtatgtg ggaattattg aagcggtgtt
      541 aggtgtgtag taattgttga attagacgaa tgagtacaac ccttctgagc attttattag
      601 gtattggcgg catgcttggc tggggaattt atgatttcct gggcggagtt ttttccaaac
      661 agattggttc tttcaagtct ttgttctggt cgcaattggc gggattaata tccattcttc
      721 tgctggcatt tgctttcaaa acaaccttcg agattcccat tctggcactc gccttgtcgc
      781 cactggcatc catcgtctat tcggcgggat atttgttctt cttcaaggga tttgagaaag
      841 gcaatgtctc aattattgca gcgaccatga atctgtgggc ggtgttcacc atgattttcg
      901 ccttcctctt tatgggacag cgcctgagca taacccaaac cgtcggcgtg ttcatgatcc
      961 tctctggcgc aacacttgcc gccatcgatt ggaacagtat caagaatcag ggatttcaac
     1021 tttcattggg tgtccgagaa gcggttctgg gcgcattttt ctttggagtg ttttggaata
     1081 ttagcgagat tgtttctgaa aagattggct ggcttttatc cacagtgctg accaaacttg
     1141 gcatcacaat tttcttgttg gttttttccc tgtttgcaaa acaagaaacc gggctgacga
     1201 acatcccaac gaaaacaaaa accatagtct cgttgatggg tatcatcgaa gtcagcgctg
     1261 tggcgttggt caactacggc ttgacgattg gcgacgcaat cttgatcaca cccatcgctt
     1321 cggcgctgtc cattgtgacg attgcgttgg cggtcatttt tctgaaagat aaactttcaa
     1381 aacttcaatt aatcggggtt gcgaccgcca ttcttggcat catcgcaacc gcattttaga
     1441 aacggaaaat tttcatggca ttagttatct tcgattatga cggtgtcctc gcagacacac
     1501 tcgacgactt aattcacttc gggcaggaag cctgtaacca attaggcgtt aaccatgtag
     1561 tgacaaaaga cgacttgagc aatctcgagg tcatgtcctt tgcaacctat gggcgagctt
     1621 gcgaagtacc cgaacctctc atagacgagt tcgtcaaaat cagtttggat ttgttcgcaa
     1681 aaaaagcatc ccctcccgct attttcaatg gagtaaggga agtaattcag cacttctcca
     1741 acaaacataa gatcgccatc gtgaccacca attcatcgca aaacgtgcat gccttcctca
     1801 cccaacacgg actggatagt ctcgtccatg ccgtgtacgg tgtggacaca cccggttcaa
     1861 aagcacaaaa aatatccatt gctcgagagc gttttgcgaa taacggagaa gcgatcttta
     1921 tgattggcga tgcgctgagc gacatccgct cggcgaaaga agctggggtg accagcatcg
     1981 ctgttacatg ggggcatcag agtttggaga ccttgcttcg gggaagtcca gattttgtga
     2041 ttaactttcc acatgaattg atcaagatta ttgagtaata aaaggtggaa aatgaatttt
     2101 attttaccga taatacttat cctcatttcg gttagcgaaa taaataaaag cgttcttgta
     2161 ttaaggaaaa aacagaaaca tattttcttg gcttttaaaa ttctgctatc aatactcaca
     2221 ccatttctac cagacaaaag gataaacgga atacaaaaag aatataataa aagaattgaa
     2281 acctatgcaa tccaatctct ttggttttgg atttttgtgc tcacatataa tattattaag
     2341 ctatggattg cgatcttaaa gaaagacttg ttttagtgat atagacaaca agatagcttt
     2401 tgaaagccct cttccactaa aatcctccta cgtattcagg aaaaacaaat gacaactcct
     2461 caaccctccc ctacc
//
LOCUS       DAOQJE010000584         2468 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_31065_length_2468_cov_9.482801, whole genome shotgun sequence.
ACCESSION   DAOQJE010000584 DAOQJE010000000
VERSION     DAOQJE010000584.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2468)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2468)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2468
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31065_length_2468_cov_9.482801"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1662)
                     /locus_tag="PLT08_13715"
     CDS             complement(<1..1662)
                     /locus_tag="PLT08_13715"
                     /inference="COORDINATES: protein motif:HMM:NF024883.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HNE05578.1"
                     /translation="MKTPSSSTSTHHEDINDVIRRADILQRVMSTISSGLELDPLLSN
                     ILESAVKLIEATHGTIGLVGDRNGKPIIRTVATHNMPAEELGAEMAPGIGLAGKVLLE
                     KKTVLLDRYGSLEEPTLQGLAEHSVIGIPILWGEEMIGFFGIGVDARRFTDHDVETLE
                     YFAQYAAVAIHNANLFKANQHALEEMHLLYKTSQRIGLSDDVDGVIDAYLDQVAAGGK
                     YVCNICLYEFDENNERTEVIVKGRWMPETGSERLDEHLPYVQDNLDPILDAGETLIFS
                     DVRNDSRAPESLGEIQAASGRWALVMIPLMVRNQRIGLVILSHPGVHEWADEELSPYR
                     AVAAQLAIAIDHRSQQTLLQQRGQQLAVLQERQRLARELHDSVTQLIFSTTLIAQSIS
                     PAWKRNPEEGEKRVERLLELSQTALREMRSLLFELKSSEEIQDTELPVTLTGLERIRH
                     YGLLGAIRLLAQDFSADGILVSVDVDEASEKLFSKQTGSRSGKEPMVDESIYRITQEA
                     LNNAVKHAKARQIFISISCQGRQLWVSIADDGKGFKSTSITKQKPE"
     gene            2071..>2468
                     /locus_tag="PLT08_13720"
     CDS             2071..>2468
                     /locus_tag="PLT08_13720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE05579.1"
                     /translation="MYICVLTSTPDDDDIPYDPSPYMNGYRWKQHHVEPTEVEKQIKQ
                     LMDDGVDVFVNLCDGTPDDALSGIALVKALEKYNAAFTGADSKFFDPTRDEMKNAAKR
                     VSVPTPSWAFASRAEDAEKIAKRLKFPMLV"
BASE COUNT          704 a          569 c          508 g          687 t
ORIGIN      
        1 ctcgggcttt tgtttcgtaa tggatgtact tttgaagcct tttccatcat ctgcaataga
       61 aacccacaat tgcctgcctt ggcaagagat tgatataaaa atctgacgag ccttggcatg
      121 cttgacggca ttgtttagcg cctcttgagt gatgcggtaa atgctttcat ccaccatcgg
      181 ctctttcccc gacctcgaac ctgtctgctt actaaaaagc ttttcactcg cctcatccac
      241 atccacactt acaagaatac catctgctga aaaatcttgt gccaacaatc gaatggcacc
      301 taatagccca taatggcgaa tacgctccag cccggtcaat gtgacgggca actcagtatc
      361 ttgaatctct tcgcttgatt ttaattcaaa cagtagcgaa cgcatctcac gcaacgcagt
      421 ctggctcaac tccaacaagc gttctactcg cttttcgcct tcctctgggt tccgcttcca
      481 tgccggcgaa attgactgag caatgagagt cgtgctaaag atcaactgag tcacagagtc
      541 gtgcagttca cgagccagcc gctgacgttc ctgtagcaca gccagttgct gtccgcgttg
      601 ttgtaacaag gtctgttgag agcgatggtc aattgcaatt gccaattgtg ccgccactgc
      661 ccgatacggg cttaattcct cgtcagccca ttcatgcacc ccagggtgac tcaaaattac
      721 caatccaatc cgttgatttc gcaccattag cggaatcatc accaatgccc atctgccaga
      781 tgctgcctga atttcaccaa gcgactctgg cgctcgcgag tcatttctga catctgagaa
      841 gataagagtc tctcccgcat caagaatggg gtcaaggtta tcttgcacgt agggcaggtg
      901 ttcatccaaa cgttctgagc cggtttcggg catccaacgt ccttttacga tgacttctgt
      961 ccgctcgttg ttttcatcga attcataaag acaaatattg cagacatact taccgcccgc
     1021 cgcaacttga tccaaataag catcgatcac tccatctaca tcatcggaaa gtccaatgcg
     1081 ttgacttgtc ttataaagca ggtgcatttc ttcaagtgca tgctgatttg ccttgaaaag
     1141 attagcattg tgaatggcaa cggcagcgta ttgagcgaaa tactccagag tttccacatc
     1201 atgatctgta aaacgccgag catctacgcc aattccaaag aatccgatca tctcttcgcc
     1261 ccataaaatg gggattccaa tgacggaatg ttctgcaagc ccttgaagag taggttcttc
     1321 tagtgagcca taacggtcta agagtactgt tttcttttca agtaatactt ttccagctaa
     1381 ccctatccct ggagccattt ctgcgcctag ttcttcggct ggcatattat gagttgccac
     1441 tgtgcggatg ataggcttgc cattgcggtc tccaacaagc ccgatcgttc catgtgtggc
     1501 ttcaattagt ttgacggcgc tctcaaggat gttggagagt agtgggtcaa gctccaatcc
     1561 gctactgatc gttgacatca cacgctgaag aatatccgca cggcggatta catcattgat
     1621 atcttcgtga tgtgtagagg tggatgaaga aggagttttc aagtttactt gctctgccaa
     1681 taagttttac gaattatata ccaatgaata gattgatttg aatgaaattg tagactgaaa
     1741 cttatatgca gagttggtgt taattacaca agtaaacatc aaacagagac gttctttttc
     1801 ttaaggacgt taataacctt aaaaacctta acgactttat gaataaacat tataaaaaga
     1861 tgaataaagc tttcaaatat tttaagataa aaccgtgtgt aaaaaccttt acataaacaa
     1921 atagttgtat acaataatca tatattgatg ataatttaaa tatttttcat catatgatga
     1981 tatgtaaaac gtttagattg atagatagca tgcgacatga agataaataa aaaatcatga
     2041 acgcaaaaaa ggaatgacaa aggaaatatc atgtatatct gcgtactaac aagcacacct
     2101 gatgacgacg acatcccata tgatccttca ccatacatga atggatatcg ttggaagcag
     2161 catcatgtgg aaccaaccga agttgaaaaa caaatcaaac aattgatgga tgatggcgtg
     2221 gatgtgttcg tcaatttgtg tgatggtaca cccgatgatg cactttcagg aattgccttg
     2281 gtcaaggcgc ttgaaaaata taatgctgcc tttactggtg cggattcgaa attttttgac
     2341 cccactcgcg atgaaatgaa gaatgccgcc aagcgagttt ctgttcctac acccagctgg
     2401 gcatttgcca gccgtgcaga agatgcagag aagattgcga agcgtctcaa attccccatg
     2461 cttgtcaa
//
LOCUS       DAOQJE010000585         2468 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_31069_length_2468_cov_5.837961, whole genome shotgun sequence.
ACCESSION   DAOQJE010000585 DAOQJE010000000
VERSION     DAOQJE010000585.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2468)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2468)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2468
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31069_length_2468_cov_5.837961"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1285
                     /locus_tag="PLT08_13725"
     CDS             <1..1285
                     /locus_tag="PLT08_13725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE05580.1"
                     /translation="PNGNPPPGNPPGGGNMGGNLEDGLATATGIYTLSGSVAEETNQT
                     YTATQQDQSGVYVTDGGVLTLNNVTVNTSGETSSSDDSSFYGLNAGILAVESTITVNG
                     GTITTSGSGANGAFATGEGSVVNLSNVTITATGDGGHGVMATLGGEVNLTNVNMTTSG
                     AHSAPIATDRGSGTVNSTGGSLLTTGADSPCYYSTGVLNIENSTCTADGSEAAVIEGA
                     NSINLIDSDVTVNVPDKWGVMIYQSFSGDAEGTEGVFTMQGGSLTYTDANSPLFYVNN
                     STGYITLDDVAVNVASGILVNAAANDRWGTSGQNGGTVYLTATNQTLSGHFIADAISA
                     INLELTDSSSWTGAINANQEASSSSLTLDASSTWTVTADSYLTCLSDPEGISGTTIQN
                     IIGNGHTVYYDAANCKALNGITYTLVGGGELKPLQ"
     gene            complement(1357..2031)
                     /locus_tag="PLT08_13730"
     CDS             complement(1357..2031)
                     /locus_tag="PLT08_13730"
                     /inference="COORDINATES: protein motif:HMM:NF027484.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NBR1-Ig-like domain-containing protein"
                     /protein_id="HNE05581.1"
                     /translation="MRHENKLNKNLLIGLILVISLSACAPAATEPSENEGPTATSTFD
                     SAIATAVANAFTAAAAVTTNTPTQTSTPAFTATALPTFTFTLAPATETPQPAPPTVLS
                     DYDFCDNSAYVSDITIKDGTVLMPGETFKKIWRLQNNGTCPWRPEYTVTFVSGYNMDG
                     VTTTINQYVAPGKSAKITVAMKAPTAEGTYTGYWSLTNFDGVNFGEVVYVRVVVSKDE
                     TEEEPE"
     gene            complement(2035..>2468)
                     /locus_tag="PLT08_13735"
     CDS             complement(2035..>2468)
                     /locus_tag="PLT08_13735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE05582.1"
                     /translation="EIYLDESDWDKIEVGYPVEVEFDTLPDTLFTGTVDTVDSELSTS
                     NMSSSVHGSVALDASFTELGLPLGASASVEVISVDLKNILLVPLDALEESDTGTYTVR
                     VLKNNEVKSQAVDIGIHNELYAEIKSGLNEGDIVVTGTFEE"
BASE COUNT          649 a          549 c          612 g          658 t
ORIGIN      
        1 accgaatggc aatccgcctc ccggaaaccc gcccggtggc ggtaacatgg gtggaaactt
       61 agaagacggc ttggctacgg ctacaggtat ttacacattg agcggcagtg tggctgaaga
      121 aaccaatcaa acatatacag ccacacagca ggatcaatct ggagtgtatg tcaccgatgg
      181 tggtgtattg accttgaaca atgtcacggt taataccagc ggagaaactt cttcatctga
      241 tgatagtagt ttttatggct tgaatgcagg cattttggca gttgagtcaa cgattacggt
      301 caacggcgga acgatcacaa ctagcggctc tggcgcaaac ggtgcttttg caacaggcga
      361 aggctcagtg gttaatttga gtaatgttac cattacggca accggagatg gcggacatgg
      421 tgtgatggca actcttggcg gagaggtaaa tctaactaac gtgaatatga ccacttctgg
      481 tgcccattct gctcctattg ctaccgatcg cggcagtggt acagtcaact ctactggcgg
      541 ttctttactt acgaccggag cagattcacc ttgttactat tctacaggtg tcttgaatat
      601 tgaaaatagc acgtgcacgg cagatggttc cgaagccgcc gtgattgaag gtgctaactc
      661 cattaatcta atcgatagtg atgtgacggt taatgtccca gataaatggg gcgtaatgat
      721 ctatcagagc ttttctggtg atgcagaagg aactgaaggt gtctttacca tgcaaggcgg
      781 gtcgttgaca tacacagatg ccaatagtcc cttgttctat gtaaacaatt caaccggata
      841 cattactctc gatgacgttg cggtaaatgt tgcatctgga atcctcgtga atgcggctgc
      901 aaatgatcgg tggggcacaa gtggacagaa tggtggaaca gtgtatttaa ctgccaccaa
      961 ccagaccctg agcggtcact ttatcgcaga tgccattagt gccatcaatt tagaattgac
     1021 tgatagctcg tcatggactg gagccatcaa cgcaaaccag gaagcgagtt cttcttcact
     1081 gacgttggac gcctcaagta cttggaccgt gacagctgac tcctacctta cttgtttgag
     1141 cgaccccgaa ggcatctcgg gaacaaccat tcagaatatc attgggaacg gtcacaccgt
     1201 atattatgat gccgcaaact gtaaagcttt gaatggtatt acctataccc ttgttggagg
     1261 tggtgaacta aaaccacttc aataagtaat tccaactctt aaacaagaga agcgcttcca
     1321 tagaatggaa gcgcttctta tattgtacga ataacgttat tcgggttctt cttctgtttc
     1381 gtccttagac acgaccacac gcacataaac gacttcacca aaattgacgc catcaaaatt
     1441 agtcaatgac cagtatccgg tatatgtccc ttcggcagtt ggggctttca tggcaactgt
     1501 aatcttggct gattttcctg gggctacata ttggttaatg gtggtggtta caccgtccat
     1561 gttgtatccg ctgacaaagg tgactgtata ttccgggcgc catgggcagg tgccgttatt
     1621 ctgaagacgc catatctttt tgaaagtttc ccccggcata agcacggtcc catctttgat
     1681 agtgatgtca ctcacatagg ctgagttgtc gcaaaaatca taatcactaa gaaccgttgg
     1741 cggcgcgggt tgaggcgttt ccgtcgcagg ggcaagcgta aatgtaaaag taggtaaagc
     1801 ggtggctgta aatgccggcg ttgatgtttg ggtaggagta ttggttgtta cggcagcagc
     1861 agcggtaaaa gcgtttgcaa ccgcagttgc aatggctgag tcgaaggtgc ttgtggctgt
     1921 gggaccctcg ttttcactgg gctctgtggc tgctggcgca caagcagaca aactgatgac
     1981 caaaatgaga ccgataagaa gatttttgtt tagcttgttt tcatggcgca tgatctactc
     2041 ttcgaatgtg ccggtaacga caatatcacc ttcgtttaaa ccggatttga tttcggcgta
     2101 caactcgtta tgaataccaa tatcaacagc ttgcgatttt acttcattat tttttaggac
     2161 acgaacagta tatgtgccag tatctgattc ttccagcgca tcaagcggga cgagcaaaat
     2221 attcttcaag tcaacgctta tgacttcaac tgaagcggat gctccaagag ggagacccag
     2281 ttctgtaaag gaggcatcta gagctacgct accgtgcacc gaagaagaca tattgctggt
     2341 ggatagctcg ctatctactg tgtctacggt gccggtaaat agagtatccg gcagggtgtc
     2401 gaattcgact tctaccggat aaccgacctc tatcttatcc caatctgact catccaagta
     2461 gatttcca
//
LOCUS       DAOQJE010000586         2462 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_31196_length_2462_cov_5.528043, whole genome shotgun sequence.
ACCESSION   DAOQJE010000586 DAOQJE010000000
VERSION     DAOQJE010000586.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2462)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2462)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2462
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31196_length_2462_cov_5.528043"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            118..432
                     /locus_tag="PLT08_13740"
     CDS             118..432
                     /locus_tag="PLT08_13740"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015925.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative quinol monooxygenase"
                     /protein_id="HNE05583.1"
                     /translation="MYIFQVHHYIKPEGIDSYKAVTLENVQKTRLEPGVLRFEFFQDA
                     VNPCHFSLFEVYADMAARDAHLQTEHFLKWKDVYLATQERKGDGHEFIAVYPEEGEWK
                     KD"
     gene            complement(476..1303)
                     /locus_tag="PLT08_13745"
     CDS             complement(476..1303)
                     /locus_tag="PLT08_13745"
                     /inference="COORDINATES: protein motif:HMM:NF014566.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II CAAX endopeptidase family protein"
                     /protein_id="HNE05584.1"
                     /translation="MYKSISEWMRTNPIPAFFLLTFVIAWMIWLPIGYLAPKYFLLAI
                     IPGAWAPTIVSVLLTWLTDGKSGVRQFLSRVIRWRVSFQWYLVVLFGIAVIAFAAQGL
                     NIWLFDGEPVKLNLPAGIPENGLLIALPIIFFVNIFFGGPLAEDIGWRGYALPKLREK
                     MSAFTASLLIGIVWVLWHLPFFWIPEGKIAVGGIPLLWFALLTTAWSVLFAWVYVNTE
                     SILMPVLFHAAVNTTLGTLGILGQANGSLTPVILNTVLTWLLVGVIVFFTGRDLKKK"
     gene            complement(1359..1973)
                     /locus_tag="PLT08_13750"
     CDS             complement(1359..1973)
                     /locus_tag="PLT08_13750"
                     /inference="COORDINATES: protein motif:HMM:NF019960.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HNE05585.1"
                     /translation="MRRTKEEAAVTRATLLKAALTLFSTKGYAATSIDDITQAAKLTR
                     GALYHHFTNKADLYNTLIEDVSARGADIVQGAVAEGGTLLDILKRVFVRQLAYIEESK
                     EARAVMELALVKTGAHPELQSGRDRQIESGKLMVASLAQTMQIGIASGELREDIPPME
                     MARSFIAFQNGVIQLWLASPKSFSLKGRAAVFADVLIQGLKKIA"
     gene            complement(2016..>2462)
                     /locus_tag="PLT08_13755"
     CDS             complement(2016..>2462)
                     /locus_tag="PLT08_13755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE05586.1"
                     /translation="SKLLAYVNWERPVRGSDVEAVCIVTSQQSVFDFVDALSQGNAKT
                     AQRLLHRLLENEDPFSLWGMVVRQFRLLLQAREILDARGNKDDVARALAIHPYVAEKT
                     TGQAGRFSMEALEGIYHRLLRIDEQVKTSQVTLDLALDTLVVELAR"
BASE COUNT          698 a          624 c          535 g          605 t
ORIGIN      
        1 tattggcact catggcattt agcgccatcc ctacaggaaa ttaacctctt gcaatgatat
       61 tgcatagttt gcctctattc gtttctatta aagtagaaac aacaatcgga ggctgcgatg
      121 tatatatttc aagttcatca ttacattaaa cctgaaggaa ttgactccta caaagctgtc
      181 acgctggaaa atgtgcaaaa gactcgcctg gagcccggtg tgttacgttt tgaatttttc
      241 caagatgccg tcaacccctg tcactttagt ctctttgaag tctatgcgga tatggctgcc
      301 cgagatgcac acctgcaaac agaacacttc ctgaaatgga aagatgtata cctcgccacg
      361 caggagcgca aaggcgatgg gcatgaattt attgcggtct atccagaaga aggggaatgg
      421 aaaaaagatt aacacttgag ccagcttttt agaaagctgg ctttttttac ttcatttatt
      481 tctttttcaa atctctgccg gtaaagaata cgatcacacc tactaacagc catgtcaata
      541 cagtgttcaa gatgacagga gtcagacttc cattagcttg acccaatatg cccaatgttc
      601 caagcgttgt attgacggca gcatggaaca agaccggcat aagaatgctt tctgtgttga
      661 cataaaccca ggcaaacaga acagaccacg ccgtcgtcag aagcgcgaac catagaagcg
      721 gaatcccgcc cacagcaatc tttccttccg ggatccaaaa gaaaggcaaa tgccagagca
      781 cccacacaat gccgatcaat aggcttgctg tgaaggcact cattttctcg cgcagttttg
      841 gtaaagcata gccacgccag ccgatatctt ctgccaatgg tccgccgaaa aaaatattca
      901 caaagaaaat gatgggcagg gcaatcaaca atccattctc ggggatacct gcaggaagat
      961 ttaacttaac cggttcgcca tcgaatagcc agatgttcag tccttgtgca gcaaaggcga
     1021 tgactgcaat gccaaaaaga actaccagat accattgaaa actaacgcgc cagcggatga
     1081 ccctgctcaa gaattgacgg acgccactct ttccatctgt gagccatgtc aacaatacag
     1141 atacgatcgt cggcgcccat gcaccaggga tgatcgccag caagaaatat ttcggcgcga
     1201 gatagccaat ggggagccag atcatccatg cgataacaaa ggtcaacaaa aagaaggcag
     1261 ggattgggtt cgtcctcatc cattcggaaa ttgatttgta catggtattc tcctttttac
     1321 ttttacatac tatttagtat gtaaattgaa taaaaaaatt aggcgatctt tttaagtccc
     1381 tggatcaaca catcggcaaa gaccgccgcc ctacccttca acgagaaaga tttcggcgaa
     1441 gcaagccata gctgaataac accattctga aatgcaataa aagaacgagc catttccatg
     1501 ggaggaatat cttcgcgtaa ctcaccactg gcaatgccga tctgcatggt ttgagcaagg
     1561 cttgcaacca tcaacttgcc tgattcaatt tgccgatctc gccctgattg cagttcagga
     1621 tgcgcccccg ttttgaccaa tgccagttcc atcacagcgc gcgcttcttt gctctcttca
     1681 atataagcaa gctggcgaac gaacacccgc ttcaagatat caagtaacgt tccaccttct
     1741 gccacagcac cttgcacaat gtctgctccg cgggcagaga catcttcgat caacgtgttg
     1801 taaagatcgg ctttgttggt gaagtgatga tacaacgcac cacgagtcaa ctttgcagct
     1861 tgggtgatgt catcaatcga cgtcgccgca tagcccttgg tgctaaaaag ggttagggca
     1921 gctttcaaaa gggtggcgcg ggtgactgcc gcctcttctt ttgtacgtct catatataca
     1981 tactaatcgg tatgtaattg tatgtcaaga gagatttatc gtgccagttc aacaaccagc
     2041 gtatcaagag ctaggtctaa tgtgacctga cttgttttta cctgctcatc gatccgcagc
     2101 agacgatggt aaattccctc caaggcttcc atcgaaaaac gccctgcctg cccggtggtc
     2161 ttttcggcaa catatgggtg gatcgccagc gcgcgcgcca catcatcttt attaccgcgc
     2221 gcatctaaaa tttcgcgtgc ttgaagcagc aaccgaaatt gacgtaccac catcccccac
     2281 aacgagaatg ggtcttcatt ctctaacaaa cgatgcagta acctttgcgc cgtcttggca
     2341 ttgccttggg ataaggcatc gacaaagtcg aacacacttt gctgggatgt gacgatacaa
     2401 accgcctcca catcggaccc gcgaacgggt cgctcccagt tgacatatgc cagcaacttt
     2461 ga
//
LOCUS       DAOQJE010000587         2462 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_31202_length_2462_cov_3.790195, whole genome shotgun sequence.
ACCESSION   DAOQJE010000587 DAOQJE010000000
VERSION     DAOQJE010000587.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2462)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2462)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2462
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31202_length_2462_cov_3.790195"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(197..2173)
                     /locus_tag="PLT08_13760"
     CDS             complement(197..2173)
                     /locus_tag="PLT08_13760"
                     /inference="COORDINATES: protein motif:HMM:NF021375.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2075 domain-containing protein"
                     /protein_id="HNE05587.1"
                     /translation="MRRAYYSDKIPDFQKRTSDEILGRLAQGNDFSLEQTQRDAWLEE
                     IRILKNILQSYKGSIYFEYSIPRMGKRIDVVLVIGAVIFVLEFKVGEKDFPSHAIDQV
                     WDYALDLKNFHETSHDSYIAPILISTNAKASTATVATTAHNDKLLIPIKCNEKLLPQV
                     LKDVLLFSDGDEINSSHWEAGRYQPTPSIIEAAMALYSGHSVDEISRSDASAINLSQT
                     SDAVSEIIQFSKQNSIKSICFVTGVPGAGKTLVGLNIATKHIDKDNDLYSVFLSGNGP
                     LVDILREALTRDKVQREKERGRKIKKGEVMSEVKMFIQNVHNFRDEGLLDLQKPPIEH
                     VALFDEAQRAWDLQQTVNFMQRKRGHSNFHASEPEFLISCLDRHPDWAVVVCLVGGGQ
                     EINTGEAGISEWIESLERSFPDWHIYISPRLTDSEYGTEKVLEKIRGHKKITFKDELH
                     LSVSMRSFRAEHVSLLVKQILDLQPDDARKTLEKIQDKYPIVITRDLAKAKQWLKAQA
                     RGSERYGIVVSSQAERLKPHAIDVKSPVDPIHWFLDGKEDVRSSYYLEDVVTEFQVQG
                     LELDWACVTWDADFRHTKDAWEHFSFVGKKWNRIRKIERQNYLKNAYRVLLTRARQGM
                     VIVVPAGDEEDATRSKEFYDPIFEYLLKIGFGVI"
BASE COUNT          691 a          516 c          473 g          782 t
ORIGIN      
        1 cgccaaagcc tgcacccaac actaaaactt ttgtcttcat ctgcctctct ccattactga
       61 tttgatcgaa atttaatcgg attgtagctc aatctaagac aaaaaggatt tcacgaaggt
      121 gacacgattc cctttgatga cgggatttcc gtttgcgcgg gtgagttgga ggaggttggt
      181 ggtcatgggg agatagttat attacgccaa atccaatctt taataaatat tcaaaaattg
      241 ggtcataaaa ctctttgcta cgtgtagcat cttcttcatc tccagctggg acaacaatta
      301 ccattccctg acgcgctcgc gtcagaagaa cacgataagc attctttaga taattttgcc
      361 tttcaatctt cctgattcga ttccattttt tgcctacgaa agagaaatgc tcccaagcat
      421 cttttgtgtg gcgaaaatct gcatcccaag ttacacatgc ccaatcgagt tcgagacctt
      481 gtacttgaaa ttctgtaaca acgtcttcaa ggtagtatga agagcgaacg tcttctttcc
      541 catcaagaaa ccagtgaatt gggtcaactg gtgatttgac atcaatagca tgaggtttaa
      601 gtctttcagc ctgagaagaa acaacaattc catatctctc tgaccctcgt gcttgtgctt
      661 ttagccattg ttttgctttt gcaagatcac gggtaatcac gataggatat ttatcttgga
      721 ttttttccaa agttttcctt gcatcatcag gctgcaaatc caaaatctgc ttaactaaaa
      781 gtgatacgtg ctctgcccga aaagaacgca ttgaaacgga gaggtgcaat tcgtccttga
      841 aggttatttt tttatgacct ctaatttttt ctaaaacttt ctcagttccg tattcactat
      901 ctgtaagacg aggggaaata taaatatgcc agtctgggaa tgagcgttca agagattcga
      961 tccattcact aatgccagct tccccagtat taatttcttg tccgccacct acaaggcaaa
     1021 caacaactgc ccaatctgga tggcggtcaa gacatgaaat tagaaattct ggttctgaag
     1081 catggaagtt tgaatgaccg cgttttcgct gcatgaagtt gactgtttgt tgcaaatccc
     1141 aagctcgttg agcttcatca aacaatgcaa catgctcgat tggaggtttt tgcaagtcta
     1201 atagaccttc gtcgcgaaag ttatgaacat tttgaatgaa catcttaact tcgctcatca
     1261 cttccccttt tttaattttg cgtccacgtt ccttttctct ttgaactttg tctcgtgtca
     1321 atgcttctcg caaaatatcc acaagcggtc cattgccaga aagaaaaaca ctgtaaagat
     1381 cattgtcttt gtcgatgtgt ttggttgcga tgtttaagcc tactagagtt ttacctgcac
     1441 caggtacgcc agttacaaaa catatcgact taatagaatt ctgttttgag aactgaatga
     1501 tttctgatac tgcgtcggat gtttggctga gatttattgc gcttgcatca ctgcgagaaa
     1561 tttcatctac agaatgccca ctatacaatg ccattgctgc ttcgataata cttggggtag
     1621 gttgataccg cccagcttcc caatgacttg aattaatttc atcgccatct gaaaaaagta
     1681 aaacatcttt taatacttga ggcagtaatt tttcattaca ctttataggg ataagtaatt
     1741 tgtcgttatg ggcagtagtc gctacagttg cagtacttgc ttttgcattg gtagaaatta
     1801 ggattggcgc aatgtaagaa tcatgactcg tttcatggaa gttttttaaa tcaagtgcat
     1861 aatcccaaac ttgatcaatc gcgtgcgaag gaaaatcttt ttctccgacc ttgaattcaa
     1921 gaacaaaaat tacggcgcct ataacaagaa caacatcgat tctttttccc attcttggaa
     1981 ttgagtattc aaaataaatc gaacctttgt aagattgtag aatatttttt aaaatacgga
     2041 tttcttctaa ccaggcatca cgctgtgttt gttctaagga aaaatcattc ccttgtgcaa
     2101 gtctgccaag gatttcatca ctagttcttt tttgaaaatc agggattttg tcagaataat
     2161 atgctcgtct catttaaccc tctctttgag agaatatctt tgattagaca aggatacagc
     2221 taaactaagg gttgagcaag ttcaatatta ataaaaaaga gacagtcact ttttgaaggt
     2281 gactgtctct ttgattctac tgaaacgtaa tctcttccac gcgccagttc tcttcgccca
     2341 tcagcttctt caggcgctcg agcagttcgg ggcaaatgcg cgtggtgtcg ttcgggaagt
     2401 cgatcaagtg tcccttgccg ttctcaaaga tctgaaaact aaacttgtcg cgtccgtgat
     2461 ac
//
LOCUS       DAOQJE010000588         2454 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_31375_length_2454_cov_6.451021, whole genome shotgun sequence.
ACCESSION   DAOQJE010000588 DAOQJE010000000
VERSION     DAOQJE010000588.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2454)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2454)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2454
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31375_length_2454_cov_6.451021"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..692
                     /locus_tag="PLT08_13765"
     CDS             <1..692
                     /locus_tag="PLT08_13765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE05588.1"
                     /translation="MHKLESIADQIRKNFDARTAARDQALAQARQLTRACSLAIRAVH
                     RDDTESMQAQLLEAHRLADTLRNSLVSYPDLFHAGYTQDALKEFVEANVTCALIKSQP
                     LPTPEELGVEPATYLNGLAEVVGELRRRTMDILRHGYSAEAERLMGVMDEIYSVLVTM
                     DYPDAITNGLRRQTDLARGIIEKTRGDITFSLRGGHLEKAIGRLLKQMNGEQAQGPEG
                     SEAFPPMDEDD"
     gene            695..1312
                     /locus_tag="PLT08_13770"
     CDS             695..1312
                     /locus_tag="PLT08_13770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE05589.1"
                     /translation="MAAKKGGRRYPLVIFTHMMSRWWTAVFTLGLAMLGLAWAIRSWG
                     FEQWRWMTFAVLGGFTALAGILMWLFRKSAYVQPFADHLKLVTPFLRMNISYKRFRRT
                     STATMGGIFPPKSLPRSLQEIVEPIAKMSAIVIEVSALPMSQNALRLFLSPLFFKDKS
                     PHIIILVSDWMRFSAEMDSMRHGGADVPNVAHRRDISILSKLPKK"
     gene            1312..1722
                     /locus_tag="PLT08_13775"
     CDS             1312..1722
                     /locus_tag="PLT08_13775"
                     /inference="COORDINATES: protein motif:HMM:NF016874.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside 2-deoxyribosyltransferase"
                     /protein_id="HNE05590.1"
                     /translation="MNIYFACSITGGRELESFYQQFVAALEADGHVIPTSHLAQSEAM
                     EGERMLTPVDVYERDVKWVRECDVLIAEVGVPSHGVGYEIGYALNAGKPVLCLAQNGR
                     RVSKMITGNSALEMKTYSTLEEAITLARNFLSNI"
     gene            complement(1939..2292)
                     /locus_tag="PLT08_13780"
     CDS             complement(1939..2292)
                     /locus_tag="PLT08_13780"
                     /inference="COORDINATES: protein motif:HMM:NF017690.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HNE05591.1"
                     /translation="MSVAPKSQILHIAEMVSYQEGSVVSRQITKEDAGNVTLFAFDKD
                     QGLSEHKAPFDALVHILEGEADVTISGTPYRLSAGDAIIMPANEPHALKAIQKFKMLL
                     TMIRATSVAESGVKL"
BASE COUNT          607 a          577 c          622 g          648 t
ORIGIN      
        1 ccatgcacaa acttgaatcc atcgccgatc aaattcgcaa gaattttgat gctcgtactg
       61 ccgcacgtga ccaagcattg gcacaggcgc gtcagttaac ccgcgcttgt tcacttgcga
      121 tccgtgcggt acaccgtgac gataccgaaa gcatgcaagc ccaacttctc gaagcccatc
      181 gtctggcaga cacccttcgc aattctctcg tctcataccc agacctcttc catgctggat
      241 atacccaaga cgcgctcaaa gaatttgtgg aagccaatgt aacctgtgca ttgatcaaaa
      301 gccagccgct tccgactccg gaagaattgg gtgttgaacc cgcaacctat ctcaacgggt
      361 tggcagaagt ggtcggtgaa ttgcgccgcc gcacgatgga tattctccgt cacgggtatt
      421 cggcagaagc cgaacgcttg atgggcgtga tggatgaaat ctactctgtg cttgtcacca
      481 tggattatcc cgacgccatt acgaacggac ttcgccgtca gaccgatctt gctcgcggca
      541 ttatcgaaaa gacgcgcgga gatatcacat tcagtttgcg tggcgggcat ttagaaaaag
      601 ccattgggcg tttgctcaag caaatgaatg gcgagcaggc tcaaggtcca gaaggaagcg
      661 aagcatttcc tcccatggat gaggatgatt aaccatggca gcaaaaaaag gcggacgccg
      721 ctatcctctt gtcattttta cacacatgat gagccgttgg tggacggcgg tctttactct
      781 tggcttggcg atgcttgggc tggcgtgggc aattcgtagt tgggggtttg aacaatggcg
      841 ttggatgacc tttgcggtgt tgggcggttt tactgcattg gcaggaattc tgatgtggct
      901 cttccgtaag agtgcgtatg tgcaaccatt tgcagatcat ctaaaactgg tgacgccatt
      961 tttgcgaatg aatatttcgt ataaaagatt tcgtcgcact agcactgcca ccatgggcgg
     1021 aattttcccg ccgaaaagct tgccgcggtc tctgcaggaa attgttgaac ccattgcaaa
     1081 gatgagcgct attgtgatcg aagtgagtgc tttgccaatg tcgcaaaatg cgttgaggct
     1141 tttcctttca ccgcttttct tcaaagataa atctccgcat atcatcatcc ttgtttcaga
     1201 ctggatgcgt ttcagcgctg agatggatag tatgcgtcac ggcggggcgg atgtgcccaa
     1261 tgttgcacat cgtcgcgaca tttccattct ttcaaaactc ccgaagaaat aatgaatatt
     1321 tattttgcat gttctatcac gggtgggcgt gagttggaat ctttttatca acaatttgtt
     1381 gccgcccttg aagccgatgg gcatgtgatc cctacttcgc atctggctca atctgaggcg
     1441 atggaaggcg agcgtatgct tactccggta gatgtgtatg aacgcgatgt gaagtgggtt
     1501 cgagaatgtg atgttttgat cgcagaagtg ggagtccctt cccatggcgt gggctatgag
     1561 atcgggtatg ccttgaatgc tggcaaacct gtgctttgct tagcacaaaa tggaaggcgt
     1621 gtttctaaaa tgatcactgg gaattctgcc cttgaaatga agacatattc cacccttgaa
     1681 gaggcaatta ctctggcacg taatttcttg agtaacatct gatcttatgt gcttcatcat
     1741 gtgcaatggg tgacatatta cgatgcccaa aaaacggaac ctgcattaat atcactcacg
     1801 gagacggttg tagacacctc tcaacgcaga attccagtct cctcctttgg cgaaagctga
     1861 cgtcaggtga acagcctgac gtcagcattt taattcttgt tagcgcgcgg gcacgtccaa
     1921 caactataga ttttatgtct acagtttcac cccactctcg gcaacagatg ttgctcggat
     1981 catagtcaac aacatcttga atttctgaat agctttcaaa gcatgtggtt cattggcggg
     2041 cataatgatg gcatctcctg cggagagacg atagggtgtg ccggagatgg tgacatccgc
     2101 ttcgccttcg aggatgtgga caagagcatc gaagggagct ttatgctcgc tcaagccttg
     2161 gtctttatcg aacgcaaaca aggtgacatt gcccgcatct tcttttgtaa tctggcggct
     2221 gaccaccgag ccttcttgat aactaaccat ttcagcgata tgcagaattt gtgatttggg
     2281 tgcaacagac ataaggttct ccaagggtga caaactattt ttcttctggc ggtttgacgg
     2341 cgctaaactc acgccagagt aacaggtcgt aaataatctt taatccacca cagaggaaga
     2401 atggaacgct taacagaccc ggaacactga aaaagattcc ggtcaatgcg gggg
//
LOCUS       DAOQJE010000589         2450 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_31456_length_2450_cov_6.311482, whole genome shotgun sequence.
ACCESSION   DAOQJE010000589 DAOQJE010000000
VERSION     DAOQJE010000589.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2450)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2450)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2450
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31456_length_2450_cov_6.311482"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..614)
                     /locus_tag="PLT08_13785"
     CDS             complement(<1..614)
                     /locus_tag="PLT08_13785"
                     /inference="COORDINATES: protein motif:HMM:NF014051.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HNE05592.1"
                     /translation="MNKLKELLRSKLASASALLMLSMTVVNAGNYIYNLIMGRWLGPS
                     LFADLSLIVTLLLVVTFLTAPIQMTAARYSAIHTADGDDKTLASLRRFIWFGALGVGL
                     TLTVLFAIFATALKGFFHTQSALPFVIFGAALPFSMLQAVERGMLQGRTSFKVLAISY
                     QVEMWSRLLVSILLVALGLSVNGAVIGISLSFVFTWLVARSAVVG"
     gene            complement(607..981)
                     /locus_tag="PLT08_13790"
     CDS             complement(607..981)
                     /locus_tag="PLT08_13790"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNE05593.1"
                     /translation="MSKAKILVVDDEPNNQRILTYTLNKAGYESAAIADGENTLVWLA
                     SNTPDLAILDVSMPGMDGITLLTHIRSMPQYSRLPVIILTGSGDDDERLRAEGLGIQA
                     FLTKPASSKTVLEAVANLLPHE"
     gene            complement(983..1327)
                     /locus_tag="PLT08_13795"
     CDS             complement(983..1327)
                     /locus_tag="PLT08_13795"
                     /inference="COORDINATES: protein motif:HMM:TIGR00377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HNE05594.1"
                     /translation="MSLEINSTEQVIKIAAITLKGRLDAFEAPALRKTCDAYIADEFS
                     HFVFDLTEVAMLDSAGLAVLVSVLKRARLKNGDVRLVWPREEAASRILKLTKFDQVFV
                     SIEKGEIVPRGF"
     gene            complement(1352..>2450)
                     /locus_tag="PLT08_13800"
     CDS             complement(1352..>2450)
                     /locus_tag="PLT08_13800"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HNE05595.1"
                     /translation="PQVSGVDIFATSIPAFEVGGDFFDVIDRSAESLIFSLGDVTGKG
                     MPAALLMSMAHTIIKAASRNMPFDHPHQVLNRLNHDMESDFSNVGMFTTVMLGMVDCA
                     NCKLHFSNAGQSPIYYMPVNGDAVLLEAQDIPVGILDTVEYTTQSLSLAHGDLFVAAS
                     DGFPESRNPVGEMYGYERMKNILSASRQLSSKQIAEALLNDVNTFCETHPQDDDRTII
                     VLKFEKQNEKQIMFNDSLTVRANYEDIRQPCERLRTNLSQSGVSEEIAGLCELALQEL
                     LTNLVDHAYEGNLNGQITVRMASKNGQIFLQTEDTGLPARIKMEDVHMPNPEDLAEGG
                     YGMAIIRSLMDDIRYESMQGKNIWTLIKNLK"
BASE COUNT          684 a          583 c          572 g          611 t
ORIGIN      
        1 cctactactg cagagcgcgc caccaaccaa gtgaatacga atgacaagct gatgccgatc
       61 acagctccat tgaccgaaag ccccaaagcg accagaagga tgctgaccaa caaacggctc
      121 cacatctcga cttgataact gattgccaaa actttgaaac tggtgcgccc ctgaagcatt
      181 ccacgttcaa cagcctgcaa catgctgaaa ggaagtgcgg ctccaaaaat tacaaaagga
      241 agcgcagatt gtgtatggaa gaaacccttc aatgcggtag caaaaatggc aaagagaact
      301 gtcagcgtga gccctacccc caaggcgcca aaccaaataa aacgtcgaag ggaggcgagg
      361 gttttgtcat ctccatcggc ggtatgaatg gcagaataac gcgcagcggt catctgaata
      421 ggggcggtga gaaaagtcac aaccagaagc aaagtaacaa tgagtgaaag atctgcaaac
      481 agtgacggac ccagccacct gcccataata aggttataga tgtaattgcc cgcattgacg
      541 acggtcatgc taagcataag cagcgcgctg gcagatgcca gtttggagcg aagtagttct
      601 ttgagcttat tcatgaggga gtagatttgc tactgcttcc aagaccgttt tagaactggc
      661 tggtttggta agaaaagctt gaatgcccag cccttcggca cgcagacgtt catcatcatc
      721 gccactacct gtgaggataa tgacgggcaa acgagaatac tgcggcatag agcggatgtg
      781 tgttagcaaa gtaattccat ccatgcctgg catggataca tccagaatgg caagatcggg
      841 agtattagat gcaagccaaa ccaacgtgtt ttcgccatca gcaatggctg cactttcata
      901 gccggcttta ttaagggtgt aagtaaggat gcgctgattg ttaggttcat catccactac
      961 gagaatttta gctttgctca tgttaaaaac ctcttggtac gatctcgcct ttttcgatag
     1021 agacaaaaac ctgatcgaac ttggtgagtt tgagtatgcg ggatgccgct tcttcgcggg
     1081 gccagacaag acgcacatcg ccattcttga ggcgggcccg tttgagaaca ctgacaagga
     1141 ctgccaaccc agccgagtcc agcatggcta cctcggtcag gtcaaaaaca aaatggctga
     1201 attcatcggc aatgtatgcg tcacaagttt ttcgtagcgc gggggcttca aaggcatcga
     1261 gcctgccttt tagtgtgatg gcagcaattt ttatgacttg ttctgtagaa ttaatttcga
     1321 gtgacatagt tacctctttc tcttggttcc gttatttcaa attcttgata agcgtccaga
     1381 tattcttgcc ttgcatagac tcatagcgaa tatcatccat caacgaacgg atgatcgcca
     1441 tgccataacc gccttcggca aggtcttcgg ggtttggcat atgaacatct tccattttta
     1501 tacgagcagg caagccggta tcttccgttt gcaggaaaat ctgtccattc ttgcttgcca
     1561 tccgcactgt tatctgcccg ttcaaattcc cttcgtaggc atggtcaacc aggttcgtaa
     1621 gtagttcttg caaggcgagt tcgcacaagc cggcaatctc ttctgaaacc ccgctctgac
     1681 ttaaatttgt acgcaagcgc tcacatggct ggcgaatatc ttcataattg gcacgaacag
     1741 ttagactgtc attgaacatg atctgtttct cgttttgttt ttcaaattta agaacaataa
     1801 tggtgcggtc atcatcttgt ggatgagttt cgcaaaaagt atttacatcg tttaacaggg
     1861 cttcggcaat ttgtttagag gacaattggc gcgaggcgga taagatattc ttcatacgct
     1921 cgtaaccata catctcccct accgggtttc gagattcggg aaagccatct gatgcggcaa
     1981 caaaaaggtc accgtgtgcc aagcttaggc tttgggtggt gtactccacc gtatccaaaa
     2041 taccgacggg aatatcttgt gcttctaaca gaactgcatc gccattaacg ggcatatagt
     2101 aaatggggct ttgcccggca ttactaaaat gcagcttgca atttgcacag tccaccatac
     2161 ctagcatcac cgtagtaaac atacccacat ttgaaaaatc agattccata tcatgattga
     2221 gacggtttaa gacctgatgc ggatggtcaa acggcatgtt ccgcgatgcg gctttgataa
     2281 ttgtatgcgc catgctcatc aacaaagcgg cgggcatacc tttccctgta acatcgccca
     2341 aagaaaaaat gagcgattct gcggaacggt caatcacatc aaaaaaatcg ccgcctactt
     2401 caaatgccgg tatggatgtg gcaaatatat ctacaccact cacttgcgga
//
LOCUS       DAOQJE010000590         2447 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_31535_length_2447_cov_2.765886, whole genome shotgun sequence.
ACCESSION   DAOQJE010000590 DAOQJE010000000
VERSION     DAOQJE010000590.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2447)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2447)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2447
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31535_length_2447_cov_2.765886"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(2..352)
                     /locus_tag="PLT08_13805"
     CDS             complement(2..352)
                     /locus_tag="PLT08_13805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE05596.1"
                     /translation="MKNVKEESKLLASLTVFRELYDKQKDVYGVIAEFLKEVIVKNGK
                     RKFNTTEITNLLNNSFDFSLPEAVVNTAIKRLNLPKEDGVYIVQTMPVLENRTVETLQ
                     EQNLTSAEILSIKK"
     gene            complement(697..1488)
                     /locus_tag="PLT08_13810"
     CDS             complement(697..1488)
                     /locus_tag="PLT08_13810"
                     /inference="COORDINATES: protein motif:HMM:NF025027.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4145 domain-containing protein"
                     /protein_id="HNE05597.1"
                     /translation="MDIEDNLRREYGDYFQCGICQTFSEYEILFAKSYEEYHYDDEGT
                     KGQGFAIIQCQKCESLSLLTIKISGELRFESLLNEEDLNEFLRDNPDILNTEFSFYSA
                     DKFDDGAQPFYALCMSILGQYPFGLSVSKDVPDQIKLDIQESFNCLAIGAVNASVIMS
                     RRVIQQIAINFGVKEDKFLGKALNELKEKNIINNELHEALIEVKNWGDGGAHPGKIQN
                     ILPEDAKKVADLMLFLVNYIYPTKRDPSPMVEELKKIRQGKSPKK"
     gene            complement(1684..1917)
                     /locus_tag="PLT08_13815"
     CDS             complement(1684..1917)
                     /locus_tag="PLT08_13815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4177 domain-containing protein"
                     /protein_id="HNE05598.1"
                     /translation="MKNWEYLYALADNHEVVEVNGAMIGTALRGFVTTYTGRPSLTDF
                     LNQSGQEGWELVGMSPMGEVGTTLRLIFKRPSN"
     gene            complement(2029..>2447)
                     /locus_tag="PLT08_13820"
     CDS             complement(2029..>2447)
                     /locus_tag="PLT08_13820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016866 - intramolecular transferase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase family protein"
                     /protein_id="HNE05599.1"
                     /translation="ALEYIAKIDEMGGAMQSIERGFMQNEIQNAAYAAQQAIEKKEQI
                     IVGVNQFTMDEVLTLERLKVDTSIELGQREKLKKLREQRDPNVVEQLRGRLVEAAKGS
                     ENLMPLFIECVEQDMTLGEICNTLRGVWGEYVAEGF"
BASE COUNT          622 a          550 c          429 g          846 t
ORIGIN      
        1 tttatttttt tattgatagg atttccgctg acgttagatt ttgttcttgg agtgtttcga
       61 ctgttctgtt ctccaaaact ggcatagttt ggacaatata aacaccatct tctttcggca
      121 agttcaaacg ctttattgca gtatttacaa cagcctcagg aagagaaaaa tcgaaagaat
      181 tattgagtaa gtttgtgatt tctgttgtgt tgaatttacg ctttccattc ttaacaatta
      241 cttcttttag gaattcagca attacaccat atacatcttt ttgtttatcg taaagctctc
      301 tgaatactgt taatgatgca agtaatttgc tttcttcttt gacatttttc atatatcttc
      361 cctctttcta taatgttgta actagccacc tattttatac agcatgctgt ataaaacccc
      421 aatttggggg tatatacagc gatttcgctg tctaataccc tatatacgtc aaattttaca
      481 cctttgtttt tgataatatc aattattcga aacgttttga taaccacact ttttgcgagt
      541 atacacgaaa atccccctct accctagcgg gcacctcccc ccaaatgcga caatgtagat
      601 cttttgtcac ctaactttta ttgtcttgtt tgaatgatcg cccgggaagt ataggcactc
      661 actaaatggc tatccttaca caaaaaaagt ataattttat tttttgggag atttaccttg
      721 tcgtattttt tttagttctt caaccatagg acttggatcc ctttttgttg gatatatgta
      781 attaacaagg aaaagcataa ggtcagctac tttttttgca tcttcaggga gaatattttg
      841 tattttccct ggatgggcac ctccatcccc ccaatttttt acttcaatta gtgcttcgtg
      901 tagttcgtta ttgatgatat tcttttcttt caattcattt aatgccttcc ctaaaaattt
      961 atcttcttta acgccaaaat taatagcaat ttgttgaatc actcgcctgc tcattattac
     1021 ggatgcattt acagcgccaa ttgctaggca gttaaatgat tcttgaatat ctaatttgat
     1081 ttggtccggt acatccttgc taacagaaag accaaagggg tattgcccta atatagacat
     1141 acatagagca taaaaaggtt gtgcgccatc atcaaatttg tctgctgaat aaaagctgaa
     1201 ttctgtgttt aagatatctg ggttatccct taagaactca tttaaatctt cttcattaag
     1261 aagactctcg aagcgtaact cacctgaaat cttgattgtt agaagactta atgattcgca
     1321 tttttggcac tgaattattg caaatccttg tccttttgtg ccttcatcat cataatggta
     1381 ttcttcatag ctcttagcaa aaaggatttc atattctgaa aaagtttggc atattccgca
     1441 ttggaaataa tcgccatatt ctcttctcaa attatcttct atatccataa ttagcctacc
     1501 tattaattga attggttaat agctttaaga gtaaattata tctcttgcta tcgcatatct
     1561 ctcccaaata tgacaattta tactttctct taatctccta acttttcatc gtcgcattta
     1621 gggaggatga gcgggtaaaa gagacagtca cctcgcaagg tgactgtctc ttttgctgat
     1681 ctattagtta cttggtctct tgaaaatgag ccgcaaggtg gttccaactt cgcccatggg
     1741 gctcatccca accagttccc agccttcctg cccagattgg ttgagaaagt cggtcaggga
     1801 gggtctgccg gtgtacgtgg tgacaaatcc tctcagcgcc gtgccgatca tggctccatt
     1861 gacttcaacg acctcgtgat tgtccgcgag tgcatacaaa tattcccaat tcttcatgtt
     1921 ctttcgttcc ttaccatttc ctctatgatt ttttctttcc tcccctaaat tcgacggtac
     1981 ttctgtcgaa tttgggggag gtgccccgat aggggcggag ggggcgggct aaaacccttc
     2041 cgccacgtac tcaccccaaa cgcctctcag cgtattacag atctcgccga gggtcatgtc
     2101 ctgttccaca cattcaatga agagcggcat caagttctcg ctgccttttg cggcttccac
     2161 caaccgtccg cgcaactgtt caacgacgtt tgggtctctc tgctcgcgta acttcttcaa
     2221 cttctcgcgc tgtcccaact caatcgacgt atcaaccttc aaacgttcca aggtgagaac
     2281 ttcatccatg gtgaattgat tgacaccgac aataatttgt tctttcttct cgatggcttg
     2341 ctgcgccgca taggctgcgt tttggatttc attttgcata aatccacgct caatggattg
     2401 catcgctccg cccatttcgt cgatcttggc gatgtactcc agtgcac
//
LOCUS       DAOQJE010000591         2444 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_31590_length_2444_cov_8.205525, whole genome shotgun sequence.
ACCESSION   DAOQJE010000591 DAOQJE010000000
VERSION     DAOQJE010000591.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2444)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2444)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2444
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31590_length_2444_cov_8.205525"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..215
                     /locus_tag="PLT08_13825"
     CDS             <1..215
                     /locus_tag="PLT08_13825"
                     /inference="COORDINATES: protein motif:HMM:NF012410.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE05600.1"
                     /translation="LGKEYEVPKKVLELNPSHAMLKNLLASDGELQTIIIEQVFDSAL
                     LVEGLHPDPSSMAERVQRIMEAALKK"
     gene            397..780
                     /locus_tag="PLT08_13830"
     CDS             397..780
                     /locus_tag="PLT08_13830"
                     /inference="COORDINATES: protein motif:HMM:NF021388.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2089 domain-containing protein"
                     /protein_id="HNE05601.1"
                     /translation="MPQIPQSCPSCASPLVVTQLTCTSCGTGVVGKFELSPFFRLSNE
                     SLKFLEVFVRNRGNVKEMERETGESYWTIRRQLDEVIQEMGIEEEPKPDDKSTSRQEI
                     LGRLSRGEINVQEATKLLSQLKGES"
     gene            780..2174
                     /locus_tag="PLT08_13835"
     CDS             780..2174
                     /locus_tag="PLT08_13835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE05602.1"
                     /translation="MNGQKERLMVLDMLAEGKITADEAEQLFKAMEEEPQENNADALQ
                     QAQDSASDSAEWVPGLSHLSKLSALSSLSSLSGLSSLSPADPESPRNARTRELLGALK
                     EAGVDHVTMSDLQEMKIHNLSADYVREMRGLGIEPDGIGEWINLRIHDISPRYVRDLR
                     DVGVTDLDVDEIVDLYLQGVSAKFIGGLRDLGINDLDIDEIKELNNHDISPKFIAEMR
                     SLGFKDLDVDELVELAIHDVSPKFVAEMRDLGLKDLDVDELVELANHDVSPKFVAELR
                     ALGLKDLDVDELIEFANHDVSPKFIAELRSLGFTDFDADDLIELANHDVTPDFIAAIR
                     EVGLKDLAADEFIDLRNQGITPGYVRAMVEAGLKDLEVDQLEELKIHNVTPKFVREVL
                     EMGFKDVDIDELVELSIHHVTPRFMRDVREKLGEDLTLEQILDIRLHGIDEDLMEELQ
                     AAGVKVKKIVKTKI"
BASE COUNT          650 a          626 c          592 g          576 t
ORIGIN      
        1 atttgggcaa ggaatacgaa gtgccgaaaa aagtgctgga gttgaaccct tcgcatgcga
       61 tgttgaagaa tttgctggcg agtgatggcg agttgcagac catcatcatt gagcaagtct
      121 ttgatagtgc gctgctggtc gaaggtttgc accccgaccc ttcgagcatg gcggaacgtg
      181 tgcagcgtat tatggaagcg gcattgaaga aataatagta gggaagaagg tcattcagtt
      241 atgaatgacc ttcttttttg cgtatagaca ttggcttgcc tgctaatacc ctcttttcgc
      301 caaataaatt tcctataaac caaaatattt tgtattacac caaaaatatt gacttaaata
      361 aaaatatttg ctattatgcg ttcattggag ccccaaatgc cacaaattcc tcaatcctgc
      421 cctagttgcg cttccccgct tgtcgtcacc cagctcactt gcacctcgtg tggcacgggt
      481 gtggtgggca agttcgaact ctcgcccttc ttccgcctct caaacgaaag tttgaagttc
      541 ctcgaggttt tcgtacgaaa ccgcgggaat gtcaaagaga tggaacgcga aaccggcgag
      601 tcgtactgga cgatccgccg tcaactggac gaagtcattc aggaaatggg tatcgaagaa
      661 gaacccaaac ccgatgacaa atccaccagc cgccaggaaa ttctcggcag gctgagccgc
      721 ggcgagatca acgtgcagga agccaccaaa cttctttccc aactcaaagg agaatcctaa
      781 tgaacggaca aaaagaacgc cttatggttt tggatatgct cgccgagggc aagatcactg
      841 cggatgaagc cgaacaactc ttcaaggcaa tggaagaaga accccaagaa aataatgcgg
      901 atgcccttca acaagctcag gactccgcct ccgactcagc cgaatgggtt cccggtctat
      961 cgcatttatc caagctgtca gctttatcgt ctctctcttc gctctcgggc ttgtcctcgc
     1021 tttccccagc ggaccctgaa agcccgcgta acgctcgcac ccgcgaattg cttggcgcac
     1081 ttaaagaagc cggtgtggac cacgtcacca tgagcgactt gcaagagatg aagatccaca
     1141 acctcagcgc cgactacgtc cgcgaaatgc gcggactcgg catcgaaccc gacggcatag
     1201 gggagtggat caacctgcgc attcacgaca tttccccgcg ctatgtgcgt gacctgcgcg
     1261 acgtgggcgt gaccgacctc gatgtagacg aaatcgtgga cttgtacttg caaggcgtct
     1321 ctgccaaatt tatcggcggc ttgcgtgacc ttggcatcaa cgacctcgac attgacgaga
     1381 tcaaagaact gaacaaccac gatatctctc ccaagttcat tgccgaaatg cgcagcctcg
     1441 gcttcaaaga cctcgacgta gatgaactgg tggaactcgc cattcacgac gtctccccga
     1501 aattcgtggc tgaaatgcgc gatcttggtt tgaaagacct cgatgtagac gaattggtag
     1561 aactcgccaa tcacgatgtc tctcccaaat ttgtggctga attgcgtgcc cttggcttga
     1621 aagacctcga tgtagacgaa ctgatcgaat ttgctaatca cgacgtctcg ccgaaattta
     1681 ttgccgaatt acgttccctc gggtttactg atttcgatgc cgacgacttg atcgaacttg
     1741 ccaaccacga tgtgactcct gatttcatcg ccgctatacg tgaagtgggg ctcaaagacc
     1801 tggctgccga cgaatttatt gacctgcgca atcagggcat cacccccggc tacgtccgcg
     1861 caatggtgga agcgggcttg aaagacctag aagtggatca actagaagag ttaaagatcc
     1921 ataatgtgac ccccaagttc gtgcgcgaag ttttagaaat gggtttcaaa gatgtagaca
     1981 tcgatgaact ggtagaactc agcattcacc acgtcacacc gcgcttcatg cgtgatgtgc
     2041 gtgaaaaact tggtgaagac ctcaccctcg aacagattct cgatatccgc ttgcacggta
     2101 tcgacgaaga cttgatggaa gaattgcaag ccgctggtgt aaaggtcaag aagattgtga
     2161 agaccaaaat atagctcgct ccacaataag aacattctat atcttgctct ggtcagggtt
     2221 tagttctgac cagagcattt ttaacgctaa aattattgtt acttgaaagg tattcttatg
     2281 aatatacaaa acgcctataa tgaatggtca gatatttatg actcgaacgt caaccgcacc
     2341 cgtgacctgg atgccgaagt gacgcgcctc attcttaacg gacagcggtt cccgtccatt
     2401 ctagagatcg gctgcgggac gggcaagaac accgagttcc ttgc
//
LOCUS       DAOQJE010000592         2440 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_31681_length_2440_cov_7.091405, whole genome shotgun sequence.
ACCESSION   DAOQJE010000592 DAOQJE010000000
VERSION     DAOQJE010000592.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2440)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2440)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2440
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31681_length_2440_cov_7.091405"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..290
                     /locus_tag="PLT08_13840"
     CDS             <1..290
                     /locus_tag="PLT08_13840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="succinate--CoA ligase subunit alpha"
                     /protein_id="HNE05603.1"
                     /translation="VLEMFEHDPKTEKVIMIGEIGGNEEEKAAEFIRTKMTKPVASFI
                     AGQTAPPGKRMGHAGAIIEGGAGTAADKVKALEAAGVKVAKHPEEIPTLLQ"
     gene            complement(397..633)
                     /gene="acpP"
                     /locus_tag="PLT08_13845"
     CDS             complement(397..633)
                     /gene="acpP"
                     /locus_tag="PLT08_13845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005779510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HNE05604.1"
                     /translation="MSDTYSELKVIIKDLLGADEEKITMEARFREDLEADSLDLVELI
                     MAFEDKFGAEISDEDAQKITTVGEAVKYIEANKK"
     gene            721..1749
                     /gene="fni"
                     /locus_tag="PLT08_13850"
     CDS             721..1749
                     /gene="fni"
                     /locus_tag="PLT08_13850"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004452 - isopentenyl-diphosphate
                     delta-isomerase activity [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 2 isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HNE05605.1"
                     /translation="MTKVAPIDQRKADHIKINLEQDVRSALTTGLENYHFTHEALPEL
                     DLNRLDTSLKLFGKTLTSPTLVSSMTGGTDEAETINLRLAEAAQECGVAMGVGSQRAA
                     IEHPEQAKTFQVRRVAPDILLFANLGAVQFNYGYGIDQCRRAVDMIQADALYLHINPL
                     QEAVQDAGDTNWVGITKKIEEVCKKLEIPVIAKEVGWGFSEKTAKVLADCGVSAIDVA
                     GAGGTSWSQVEMYRAPDEFTRRLAATFVGWGIPTSEAILNVKKAVPDMTIFASGGLKD
                     GLDIAKSIALGATLGGMAGEFLKAAAISTERAIEMMKLTKRQIEVTMFTSGAGTLHEL
                     AEKLKKKA"
     gene            1841..2122
                     /locus_tag="PLT08_13855"
     CDS             1841..2122
                     /locus_tag="PLT08_13855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE05606.1"
                     /translation="MDTEDPFEVRVGTFFVVMGMGAFLLFVISDLTDKVDFDFFFVAL
                     ILLVIGYYLRRTKAPPPPSGRFEWFKGFWGNTRWGRKGGGGGEGKDKKG"
     gene            2137..>2440
                     /locus_tag="PLT08_13860"
     CDS             2137..>2440
                     /locus_tag="PLT08_13860"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-transferase"
                     /protein_id="HNE05607.1"
                     /translation="MSKLIPLKKAIAEYVSDGDVVYAAGFTHLIPFAAGHEIIRQGKK
                     NLTLARATPDLIYDQLVAAGCAKKVIFSYMGNPGVGSLRIVRGAIERGELEWEEYSH"
BASE COUNT          672 a          636 c          560 g          572 t
ORIGIN      
        1 atgtgcttga aatgttcgaa cacgacccca agaccgaaaa ggtcatcatg atcggtgaga
       61 tcggcggtaa cgaagaagaa aaagccgctg aattcatccg caccaaaatg accaaacccg
      121 tcgcatcctt catcgcgggt caaaccgctc ctcccggaaa acgcatggga cacgccggcg
      181 cgatcatcga aggtggagcc ggaaccgcag ccgacaaggt caaagctctc gaagctgccg
      241 gcgtcaaagt tgccaaacac cccgaagaaa tccctaccct tttacaataa gaccaaaggg
      301 gcgactctca tcaagtcgcc cctttcattt tctatcaaga atccaaagag ccgccctata
      361 cagagcggct ctacggttat aatcttgaac agttaattac ttcttgtttg cttcgatgta
      421 ctttacagct tcgccgacag tcgtaatctt ctgagcatct tcatcagaga tctcagcgcc
      481 gaacttatct tcaaacgcca tgatcagctc aaccaggtcg agcgaatcag cttcgaggtc
      541 ctcacggaag cgcgcttcca tggtgatttt ctcttcatcg gcaccaagca aatccttgat
      601 gatcactttc aattcagaat atgtgtctga cataacatcc tccagttgga atttattgta
      661 aattgtaatc gcatggcttg cactgtcaag ccagcatatc aatcacagga acaccccctt
      721 atgacaaaag ttgcgcccat agaccagcga aaagccgacc acattaaaat caacttagaa
      781 caggatgtcc gctctgcgct gacaactggg ctggaaaatt accatttcac tcacgaagcc
      841 ctgccagaat tggatttaaa ccggctcgac acaagcctga agctgttcgg caaaacccta
      901 acctccccta cactggtaag ttccatgacc ggcggcacag acgaagccga aaccatcaat
      961 ttgcgcctcg ctgaagccgc ccaagaatgc ggagttgcca tgggcgtggg aagtcaacgt
     1021 gccgccatcg aacaccctga acaagccaaa acatttcaag tccgcagagt agcgccagat
     1081 attttattat tcgccaatct tggtgccgtg caattcaact acggctacgg catcgaccaa
     1141 tgccgccgcg cagtggacat gatccaagct gatgcgttgt atctacacat caatccgctc
     1201 caagaagccg tacaagatgc aggcgataca aactgggtcg gcattaccaa gaaaatcgaa
     1261 gaagtgtgta aaaaacttga aatccccgtc attgccaaag aagttggctg gggcttctct
     1321 gaaaaaacag ccaaggtctt agccgattgc ggcgtctccg ccattgacgt tgctggtgca
     1381 ggcggcacga gttggtctca agtagaaatg taccgtgccc ctgatgaatt cacacgcaga
     1441 ctggcagcta cttttgtcgg ctggggcatt ccaacctcgg aggcaatact caatgtcaaa
     1501 aaagccgtgc ccgatatgac catcttcgcc agtggcgggc tcaaagacgg cttagatatc
     1561 gccaaatcca tcgcccttgg tgcaaccctt ggtggcatgg caggtgaatt tctgaaagcc
     1621 gctgctattt ctaccgaacg agccatagaa atgatgaagc ttacaaaacg ccagatcgaa
     1681 gtaaccatgt tcacttcagg tgcagggacg ttacacgaac ttgcagaaaa gctcaagaaa
     1741 aaagcataaa aatagcggcg aaaacgccgc tatttttgta attccaatgg gggatataaa
     1801 tcacgattct ctgctttttc ccttgcggta aaatacgagt atggatactg aagatccatt
     1861 tgaagttcgc gtgggcacat tctttgtagt catgggaatg ggagcattcc tcctatttgt
     1921 aatctccgat ttgaccgaca aagttgattt tgatttcttc ttcgttgccc tgatcttgct
     1981 cgtcattggt tattatcttc gccgcacaaa agccccacct cccccttctg ggcgttttga
     2041 atggttcaaa ggcttttggg gaaacacacg ctggggtaga aaaggcggcg gtggcggtga
     2101 aggaaaagat aaaaaaggat aatccatact ttctttatgt caaaactgat tccactcaaa
     2161 aaagcaattg cagaatatgt tagtgatggc gatgtcgtct acgccgctgg tttcactcac
     2221 ctcatcccat ttgctgctgg gcatgagatt atccgccagg gcaagaaaaa cctcaccctc
     2281 gctcgcgcca ctccagacct aatctacgat caactggttg cggcgggttg tgccaagaaa
     2341 gtgattttct cctacatggg gaatcccggc gttggttcat tacgcatcgt gcgtggagct
     2401 atcgagcgtg gtgaattgga atgggaagag tattcccact
//
LOCUS       DAOQJE010000593         2433 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_31840_length_2433_cov_5.079899, whole genome shotgun sequence.
ACCESSION   DAOQJE010000593 DAOQJE010000000
VERSION     DAOQJE010000593.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2433)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2433)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2433
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31840_length_2433_cov_5.079899"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..859)
                     /locus_tag="PLT08_13865"
     CDS             complement(<1..859)
                     /locus_tag="PLT08_13865"
                     /inference="COORDINATES: protein motif:HMM:NF012906.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2 protein"
                     /protein_id="HNE05608.1"
                     /translation="MQKQSLTSLWQFRQLGSIEWLPAQVPGGVHTDLMALARIPDPFV
                     GDNEKRVAWVAESDWEYHTTFNVQADVFQRKNIWLVCDGLDTLASLTLNGKALGETNN
                     MFRQYRFDIKSLLKAEGNELTITFKSPVKFVTEKNAARPMNGVSQAIAGGPHLRKAPC
                     HFGWDWGPQLPPIGIWKDIYLEAYDSARISEVHLRQKHDNGKVTISARVEVENGNAPL
                     SATLLVTAPDGSEQAESVSLTSASGVVSLQVKNPQLWWPNGLGDQPLYQVRVLLSEGN
                     QTLDEKKYQM"
     gene            complement(910..1722)
                     /locus_tag="PLT08_13870"
     CDS             complement(910..1722)
                     /locus_tag="PLT08_13870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNE05609.1"
                     /translation="MPSEKKLYLSAKGITKTFGFGRQKTVAVDNVDLDLYEGEVVSIV
                     GESGSGKTTLSKVLLGLMNQTSGDVYFQGVKRDISTQSRKQEYWKNIQAIFQDPFSSY
                     NIFNKIDSVLLDCINMRGGKYLPREKKLEMMTEACSFVNLKFEELTNKYPFELSGGQM
                     QRLMIARIFLLQPKILLADEPTSMIDACSRATILDELMHLRKEIGMTIIFITHDIGLA
                     YYVSDSVYIMEKGKFVEGGSADEVILTPRHPYTKRLINDVPKIYEEWDLSTV"
     gene            complement(1712..>2433)
                     /locus_tag="PLT08_13875"
     CDS             complement(1712..>2433)
                     /locus_tag="PLT08_13875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNE05610.1"
                     /translation="ALNPTRKIINFIEDVILEHEMYVTKKDIYERAKKLFETLGLPPE
                     VLQKYPVELSGGMKQRTVIAISVLLLPKVLIADEPSSALDVTSQKMVIKMLKQLMDAG
                     FISSMIFITHELPLLYNVVDDIMVMYAGQIVEKGTSHEMVFDPIHPYSKGLMGSIIVP
                     EKGLRDVKLTAIPGVPPNLKKPPSGCRFAERCRFVRPECRVASVPLHEASNGRMYRCI
                     IPEAELRAIYAREQEEQKNAK"
BASE COUNT          609 a          575 c          575 g          674 t
ORIGIN      
        1 ccatttggta tttcttttcg tccaaagttt ggttgccctc acttaagagc acacgcactt
       61 ggtaaagcgg ctgatctccc aaaccgttgg gccaccataa ctgtggattc tttacttgca
      121 atgaaacaac tccgctggca gaggtcagcg aaacgctttc ggcttgctct gagccgtctg
      181 gagcagtaac aagtaaggtc gcagaaagag gcgcgtttcc gttttccact tccacccgcg
      241 cagaaattgt taccttgcca ttgtcatgtt tttggcgaag atgaacttca gaaatacgag
      301 ccgagtcata ggcttcgaga taaatatctt tccagatgcc gataggcgga agctgcggac
      361 cccaatccca gccgaaatga catggagctt tgcgcaggtg tggtccaccg gcgatggctt
      421 gcgaaacgcc gttcatgggg cgtgccgcat ttttctcggt tacaaacttg acgggtgatt
      481 tgaacgtaat ggtcagctca ttgccttcag ctttgagcaa ggactttata tcaaaacgat
      541 attggcggaa catgttattg gtttcgccaa gcgcttttcc attgagtgtg agtgacgcaa
      601 gcgtatccaa cccatcacaa acaagccaga tatttttgcg ctgaaagaca tctgcctgca
      661 cattgaaggt ggtgtgatat tcccagtcgc tttcggcaac ccaggcaact cgtttttcat
      721 tatcgcctac aaatgggtct gggatgcgtg caagcgccat gagatccgtg tgaactccgc
      781 ccggaacctg ggctgggagc cattctatag aacccaactg gcgaaactgc cataggcttg
      841 ttagagattg tttttgcatt ggcattcttc ctgaatgttt tcttctttag atttttattg
      901 ctcgtgcttt tatactgtgg agagatccca ttcctcgtag atcttcggca catcgttaat
      961 gaggcgtttg gtataaggat gccgcggagt gagaatcact tcatctgccg agccgccttc
     1021 cacaaatttg cctttttcca taatatagac actatctgaa acatagtacg ccagaccaat
     1081 atcatgcgtg atgaagatga tggtcatgcc gatctctttg cgaagatgca tcaattcatc
     1141 cagaatagtt gcgcgagagc aggcatcgat catggaagtt ggctcatcag cgaggagaat
     1201 ttttggctgc aacaaaaaga tgcgggcaat catgaggcgt tgcatttgtc cgccagaaag
     1261 ttcgaaggga tatttattgg tcaactcttc gaacttgagg ttcacgaagc tgcatgcctc
     1321 agtcatcatt tctaatttct tttcacgcgg caaatatttt ccgccgcgca tgttgatgca
     1381 gtccagcaag accgagtcga tcttattgaa gatgttatag gaagagaacg ggtcttggaa
     1441 gatggcttga atgttcttcc aatattcctg ttttctggac tgggtgctaa tgtcgcgctt
     1501 gacgccttgg aagtacacat cgcccgatgt ttggttcatc aatcccaata gcactttaga
     1561 gagggtggtc ttgccgctgc ccgactcacc cacaatggaa acaacttcac cttcatacaa
     1621 gtcgaggtct acattatcca ccgccactgt tttttggcgt ccgaacccga atgttttggt
     1681 gatgcctttt gcgctcaggt agagtttctt ttcacttggc atttttctgt tcctcctgct
     1741 cacgagcata gattgcgcgc agttcagctt ctgggatgat acagcgatac atacgtccat
     1801 tgctcgcttc atgtaaaggc acacttgcga cacggcattc cggtcgaaca aagcgacagc
     1861 gttcggcgaa gcgacaacct gaaggcggct ttttgaggtt aggtggcacg ccaggaatgg
     1921 cggtcagttt gacatcgcgc aaaccttttt caggaacgat gatcgaaccc atcaatcctt
     1981 ttgaataagg atgaatggga tcgaagacca tttcatgcga agtccccttc tccacaatct
     2041 gaccggcata catcaccatg atgtcatcca ctacgttata aagcagaggc aactcatgcg
     2101 taatgaaaat catggagcta atgaaaccag catccattaa ctgcttaagc atcttaatga
     2161 ccattttttg cgaggtcaca tcgagtgctg aagagggttc atctgcaatc aacaccttgg
     2221 gcaagagcag aactgaaata gcgatcacag tgcgctgctt cataccaccg gagagttcaa
     2281 ccgggtattt ttgtaaaact tcgggaggaa gccctaatgt ctcaaagagt tttttcgcgc
     2341 gttcgtaaat gtccttctta gtaacataca tctcatgctc gagaatgaca tcttcaataa
     2401 agttaataat cttgcgagtt gggtttagag cat
//
LOCUS       DAOQJE010000594         2431 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_31873_length_2431_cov_7.747475, whole genome shotgun sequence.
ACCESSION   DAOQJE010000594 DAOQJE010000000
VERSION     DAOQJE010000594.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2431)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2431)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2431
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31873_length_2431_cov_7.747475"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1011
                     /locus_tag="PLT08_13880"
     CDS             <1..1011
                     /locus_tag="PLT08_13880"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNE05611.1"
                     /translation="SRITTSTSLPSLLGLLETDVFPSLLIRQYAFLQVSEKGLIKLMD
                     KGIPNLTTFDIETLTQKAGEYIPSLSQQDDGPRLILPLKVGTQTLGFWLLGKRDPDDL
                     YPQVEIPILQSLANQTAIALSNILQTDQLRNIYESNIDRVEKERKRVARDLHDSVLNQ
                     LANMRNSLDQRTLPAEFLSTYDGLKQRIREIISDLRPPILDQGLAYAISELVEDMREE
                     ASHMDINLDLQTSGERIPEKNETHLFFIMREACMNVLKHANAKTLNISGNITPTRVEL
                     SIQDDGKGLKIDLQKEVNTLLEEEHFGLVGMFERARLINADLNINSNENSGTTIHITW
                     QP"
     gene            complement(1132..2103)
                     /locus_tag="PLT08_13885"
     CDS             complement(1132..2103)
                     /locus_tag="PLT08_13885"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF014069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HD-GYP domain-containing protein"
                     /protein_id="HNE05612.1"
                     /translation="MKIPTELDIHVAPIQPVLMYELKSMLQNTEDLLFVLNKDWQIVS
                     CKSRNGLLLKIHLLPAKLDIRELLPDTVRRKFDVLVDQLNNTNRPTMFESMIVLPPNV
                     VNWYEFRLIPAFDDQVVLFIWNVNNYRKTKWTISNIPTSVEKLIAGWSRSLYLRDFET
                     EDHTQRVTEMTTRLARRLGLPEEEMNNLRRGAQVHDIGKIAIPDQILLKTGKLTEEEW
                     VVMRKHPLIAVEMLEPIPEFKPALVIPRSHHEKWDGSGYPDGLVGSNIPLPARIFAFA
                     DVYDALTSDRPYRKAWSRSDALQFIRNETGHHFDPALTSEFIRLMSE"
     gene            complement(2155..>2431)
                     /locus_tag="PLT08_13890"
     CDS             complement(2155..>2431)
                     /locus_tag="PLT08_13890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE05613.1"
                     /translation="NRNSENHHTKPQQRISKATPRSNGLNRVFAAAIVNQQFCDMLLK
                     DPKEALKKGYLGETFVLTPEEISVIASIRASSLAEFARELYALTGTD"
BASE COUNT          685 a          601 c          516 g          629 t
ORIGIN      
        1 agccgtatca caaccagcac ctcccttccg agcctgcttg gtttactaga gacggatgtc
       61 tttccaagtc tattgatccg tcaatatgcc tttttgcagg tcagcgaaaa agggctgata
      121 aagcttatgg acaaaggcat cccaaatctc accacgtttg atattgaaac cctgacccaa
      181 aaagcaggag aatacattcc cagcctttct cagcaagatg acggacctcg cctcatcctt
      241 ccgctcaaag tgggtaccca aacccttggc ttttggctac tcggtaagcg tgaccctgac
      301 gacttgtatc cacaggttga gattcccatc ttacagtcgt tggcaaacca aaccgccatc
      361 gccctcagta acatccttca aaccgatcaa cttcggaaca tctacgaatc caatattgac
      421 cgagttgaaa aggagcgaaa acgtgttgcc cgcgacttac atgatagtgt tcttaatcaa
      481 ctagccaata tgcgcaacag cctcgaccaa agaacccttc ccgctgaatt tctatccacg
      541 tatgatggtc tcaagcaacg catccgtgaa atcatcagcg accttcgtcc gcccatactg
      601 gatcaaggac ttgcctatgc aatcagcgag ttagtagaag acatgcgaga agaagcaagc
      661 cacatggaca ttaatcttga cctccaaacc agtggcgaaa gaatccctga gaaaaacgaa
      721 acccaccttt tctttattat gcgtgaagcc tgtatgaatg ttttgaaaca tgccaatgca
      781 aagacattga atatctcagg aaatataacc cccacccgag tggagctatc cattcaagac
      841 gatggtaaag gcttgaaaat cgacctgcaa aaagaggtca acactttgct cgaagaagaa
      901 cacttcgggc ttgttggcat gttcgaacgt gctcggttga ttaatgcaga tctgaacata
      961 aactccaacg aaaattcagg cacaaccata catatcacat ggcagcctta ataaaacagt
     1021 cttttccctc ttaaaacatt acacgccggg ccttacaccg gcgtgcaccc caggtcccct
     1081 aatcgcaatc ggatatcccc tttcctgttc caactgcgct ggaaagcaag gttattcaga
     1141 catcaaacga ataaattcag aagttagggc agggtcaaag tgatgccccg tctcattacg
     1201 gataaattga agagcatcgc tgcgagacca tgctttacga tatgggcggt cagacgtaag
     1261 tgcatcgtaa acatcggcaa aggcgaagat tcgtgcaggc aaaggaatgt tcgatcccac
     1321 taatccatcg gggtatccag aaccgtccca tttttcgtga tgtgagcgtg gaattacaag
     1381 tgcaggtttg aactcgggga taggttctaa catctccaca gcgatcaatg gatgcttacg
     1441 catcaccacc cactcctcct cagtcaactt gccggtcttc aataaaattt ggtctgggat
     1501 ggcgatcttc ccaatatcat gaacttgagc gccacgccgg agattgttca tttcttcttc
     1561 aggcaaccct aaccgacgag ctagtctcgt ggtcatttca gtgacacgtt gtgtatgatc
     1621 ttctgtttca aaatctcgta aatacaagga acgtgaccag cccgcgatca gtttttcaac
     1681 tgaggtaggg atgtttgaga ttgtccactt ggttttgcga tagttattca cattccaaat
     1741 gaaaagcact acttggtcat caaaggcggg aattagccga aactcatacc agttaacaac
     1801 attcggcggc agaacgatca tgctttcgaa catagtaggg cgatttgtat tgttcagttg
     1861 atctaccagc acatcgaatt ttcgtcttac agtatctgga agtagttcgc gaatatccaa
     1921 tttcgcgggc aagagatgta tcttcagcaa gagcccatta cgtgacttac agcttacaat
     1981 ctgccagtct ttattcaaca cgaacaaaag atcttccgtg ttctgcaaca tggatttcag
     2041 ctcatacatc agcacaggtt gaataggtgc aacatggata tccagctctg ttgggatctt
     2101 cataccatcc gagctattca tattgtcgtg gtatgggatg ccagattgca tttcttaatc
     2161 agtcccggtg agtgcataca actcccgtgc aaattcagcc aaagagcttg cacggatgga
     2221 ggcaatcaca gatatttctt ctggcgtaag cacaaaggtt tcgccaagat agcctttttt
     2281 caaagcttct tttgggtctt ttagcagcat gtcgcaaaat tgctggttta cgattgcagc
     2341 agcaaaaact cgattcagtc cattcgaacg cggcgttgcc ttagatatac gctgctgagg
     2401 ttttgtatga tggttctctg aattacggtt g
//
LOCUS       DAOQJE010000595         2431 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_31874_length_2431_cov_5.623737, whole genome shotgun sequence.
ACCESSION   DAOQJE010000595 DAOQJE010000000
VERSION     DAOQJE010000595.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2431)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2431)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2431
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31874_length_2431_cov_5.623737"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..515
                     /locus_tag="PLT08_13895"
     CDS             <1..515
                     /locus_tag="PLT08_13895"
                     /inference="COORDINATES: protein motif:HMM:NF014420.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     component T"
                     /protein_id="HNE05614.1"
                     /translation="MWFALNQMTRMLTMAVLALPIPYTMDPNIYGTAFRQMGASDKVS
                     FTMDLAFRFLPTFARDFAITMDAQRARGYEMEKLQGGIAGRLRRLAPLIIPVVMQSTV
                     TGEEVIDAMDLRAFGTKPRTWLKDLKYAPRDYVLLGLGVGIFLACCILKWGFGIGEFF
                     VPDFFLNLFV"
     gene            complement(559..1389)
                     /locus_tag="PLT08_13900"
     CDS             complement(559..1389)
                     /locus_tag="PLT08_13900"
                     /inference="COORDINATES: protein motif:HMM:NF019533.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1684 domain-containing protein"
                     /protein_id="HNE05615.1"
                     /translation="MAEKTYRQTINEWRKERNEGIRKENAWLSLAGLYWLKLGKNQFG
                     SDPKSEILLPERIAANVGHFEYNGKSVSLRANPGQKVTVNDKATDFALLQPDISENPS
                     YIQLEDIQMVVIQRGNRMGVRMWDNKSEKRASFPARTWYDIDEAFRIPATFFAYDRPK
                     MAYFPDLTGEKSEFPVEGYVTFTLDGKEHKLDINKEEDGVLFLRFWDLTANDETYPTG
                     RYLIADVEEDGKIFLDFNRAYNPPCAFTDFATCVFAPEQNHLDVKITAGETYPRDIFK
                     "
     gene            1477..2031
                     /gene="dcd"
                     /locus_tag="PLT08_13905"
     CDS             1477..2031
                     /gene="dcd"
                     /locus_tag="PLT08_13905"
                     /EC_number="3.5.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008829 - dCTP deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0009394 - 2'-deoxyribonucleotide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dCTP deaminase"
                     /protein_id="HNE05616.1"
                     /translation="MGLKPDHWIRKMALEHGMIEPFVDKQVRQGVISYGVSSYGYDIR
                     VANEFKIFTNVFSATVDPKNFDTNSMVDFVGDVCIVPPNSFALARTVEYFRVPRKVLT
                     VCLGKSTYARCGIIVNVTPFEPEWEGYVTLEISNTTPLPAKIYANEGLAQVLFFEADE
                     ECETSYADKKGKYQGQQSIVLPKL"
     gene            2070..>2431
                     /locus_tag="PLT08_13910"
     CDS             2070..>2431
                     /locus_tag="PLT08_13910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE05617.1"
                     /translation="MANIRTEESILEELESIQRKMMQTMVRLTSIGNPQVSSINLELT
                     QLVRSLAQLKEDTKARFKRQQQRLEALAEIGGVINSSLDTKHVLDAVMDSLILLMQAK
                     RGFLMLRDETGDFQVQIAR"
BASE COUNT          588 a          495 c          642 g          706 t
ORIGIN      
        1 aaatgtggtt cgctttgaac cagatgaccc gtatgttgac catggctgtc cttgctcttc
       61 cgattcccta tacgatggac cccaatattt atgggacggc tttccgccag atgggcgcct
      121 cggataaggt ctcgtttact atggaccttg ccttccgctt cctcccgacc tttgcgcgtg
      181 attttgccat caccatggat gctcaacggg cgcgcggata tgaaatggag aaattgcaag
      241 ggggcattgc tggacgtctg cggcgacttg cacctttgat tatcccggtc gtgatgcaat
      301 cgacagtgac gggcgaagaa gtgatcgatg ccatggattt gcgagcattt ggcaccaagc
      361 cacgcacatg gcttaaagac ctgaaatatg cgccgcgtga ttatgtattg cttggtttgg
      421 gcgtcgggat tttcctcgcc tgttgtattt tgaaatgggg ctttggcata ggtgaatttt
      481 tcgtgccaga ctttttcttg aacttgttcg tctaacctga aaaatcaaaa ccgccgcttt
      541 aagcggcggt ttttttattt atttgaaaat atctcgcgga taggtttcac ccgctgtaat
      601 ctttacatcc agatggtttt gctctggggc aaagacgcag gtggcaaaat cggtaaaggc
      661 gcaaggtggg ttataggctc tgttgaagtc taagaaaatt ttgccgtctt cttcaacatc
      721 tgcaatgaga tagcgcccgg tgggataggt ctcgtcattt gcagtgagat cccagaagcg
      781 taagaagagg acaccatctt cttctttatt gatatcaagt ttatgttctt tgccatccag
      841 agtgaaagtc acataccctt caacggggaa ttcggatttt tcacctgtga ggtctggaaa
      901 atacgccatt tttgggcggt cgtaggcgaa aaaggtggca gggatgcgaa aggcttcgtc
      961 gatgtcgtac caggtgcggg cggggaacga ggcgcgtttc tcgcttttgt tatcccacat
     1021 gcggacgccc atgcgatttc cgcgttggat gacgaccatt tgaatatctt ccagttggat
     1081 atagcttggg ttttcagaga tatcgggttg aaggagggcg aagtctgtgg ctttatcgtt
     1141 gacggtcact ttttgcccgg ggttggcgcg tagtgagacg gacttgccgt tgtattcaaa
     1201 atgacctaca ttggctgcaa tgcgttcggg aagaaggatt tcacttttag ggtctgagcc
     1261 gaattggttt ttaccaagtt ttagccaata taaacctgct agtgaaagcc aggcattttc
     1321 tttgcgaatg ccttcgtttc gctctttgcg ccattcgttg atggtttgac gataggtttt
     1381 ttctgccata ttttaaaatt ataccaaaag ggttttgagt gaacgctctg ctttcatttt
     1441 tgataaaatg aacttatatc acttaaggag cagagcatgg gtctgaaacc agatcattgg
     1501 attcgtaaga tggcattaga gcacgggatg attgagccgt tcgtagataa gcaggtacgg
     1561 caaggggtga tttcatacgg tgtgtcttcg tatgggtatg atattcgggt ggcgaatgag
     1621 ttcaagatct ttacgaacgt tttctctgcc acagtggacc ccaagaattt tgataccaat
     1681 tcaatggtgg attttgtagg cgatgtttgt attgtgccgc caaattcgtt tgcgttggcg
     1741 cgtacggtgg aatatttccg tgtgccacgc aaggtgttga cggtatgttt ggggaaatcg
     1801 acctatgcac gttgtggcat tattgtgaac gtcaccccgt ttgagccaga atgggaaggg
     1861 tatgtgacgc ttgaaatttc gaacacaacg ccattacctg ccaaaattta cgctaatgag
     1921 ggtttggcgc aagtcctttt ctttgaagcg gatgaagaat gtgaaacttc atatgccgat
     1981 aaaaaaggaa agtatcaggg tcagcaatcc atcgtgttgc cgaaattata ggtttgtaaa
     2041 tatccctgag tggatatagg agattctcca tggcaaatat ccgtactgaa gaaagcatcc
     2101 tcgaagaact cgaatcaatt caacgcaaaa tgatgcagac catggtgaga ctgaccagta
     2161 ttggaaatcc gcaggtgtct tcaattaatc ttgagttgac gcaactggtt cgtagcttgg
     2221 cgcaactcaa agaagacaca aaagcgcgct tcaagcgcca acaacagcga ctggaagcct
     2281 tggctgaaat cggtggcgtg attaattcat ctcttgatac gaaacatgtg ttggatgcag
     2341 tgatggattc tctaatcctt ctcatgcaag ccaagcgtgg gtttcttatg ctgcgcgatg
     2401 aaactggaga ttttcaggtg cagatcgcgc g
//
LOCUS       DAOQJE010000596         2428 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_31940_length_2428_cov_4.095238, whole genome shotgun sequence.
ACCESSION   DAOQJE010000596 DAOQJE010000000
VERSION     DAOQJE010000596.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2428)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2428)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2428
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31940_length_2428_cov_4.095238"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1087)
                     /locus_tag="PLT08_13915"
     CDS             complement(<1..1087)
                     /locus_tag="PLT08_13915"
                     /inference="COORDINATES: protein motif:HMM:NF016817.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinylglutamate desuccinylase/aspartoacylase
                     family protein"
                     /protein_id="HNE05618.1"
                     /translation="MSITIGTAVAKRGEIVYGEYFLVQHPVGGADVLPVIIAQGNEDG
                     PVFWITAGIHGIEHAGIQVIHKLITPELVRQLRGTIVAIPTLNPAGLRTLERQAYYHN
                     GDPNRLFPDGRTPKTDPDKTPPSALEQAYGRLFEEIKNTASFYFDLHCMGGHALSFIF
                     RDRVLYRKGDEEARKRAEIVSQKMDEMARVYGHTMTADFALGKYIDEKLHRATTASVS
                     MLAGIPSLTAELGSSYTPYPEVVHAAVSGLRNVMRWSGMLDGEMEAITGIKVVDPGYA
                     TRRRSVARVTQACMVHHLKEPGDLVKVGDPLVEMRDAWGRVLGVLTSEYDGFVISRPA
                     GILFYPGEATVLLAIPDDEPLVGEYPEK"
     gene            complement(1128..1433)
                     /locus_tag="PLT08_13920"
     CDS             complement(1128..1433)
                     /locus_tag="PLT08_13920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE05619.1"
                     /translation="MNFTPLYKHLFRQPSPTDRLNLFQTLFDSNEINPDLALALLKIV
                     HSELSSEEGDGASYRQYAQIIEALRYHKMDMLRHIVETWKAGKPAKDPEWLTDGMIK"
     gene            1500..2030
                     /locus_tag="PLT08_13925"
     CDS             1500..2030
                     /locus_tag="PLT08_13925"
                     /inference="COORDINATES: protein motif:HMM:NF016840.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HNE05620.1"
                     /translation="MSHISPPESQEYAPFYSDYIQRASARGDLTAALSLQPDEMKSAL
                     GGLSDSQARFKPGPQEWSIKEVVSHLIDGERVFSYRLLRVSRKDKTPLPGFEQDDYVR
                     EAKADEIALADLIDEFTFLRRANLLAIKNMSASALAEVGTASGNPVSARALVYILVGH
                     VEHHMASLKEKYLPFA"
     gene            2092..>2428
                     /locus_tag="PLT08_13930"
     CDS             2092..>2428
                     /locus_tag="PLT08_13930"
                     /inference="COORDINATES: protein motif:HMM:NF014896.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE05621.1"
                     /translation="MPTNKHHPTAPKRPHPLTSHGVTRMDDYYWMRDRNDPETMKYLK
                     AESDYLEEVMQHTKSTQEVLFAEMKARIQETDSTVPEKRGEYFHYERFEKGNQYPIFC
                     RKKGSLEAKE"
BASE COUNT          628 a          678 c          556 g          566 t
ORIGIN      
        1 atttttcggg gtattcaccc accaacggct catcgtcagg gatagcgaga agcacggtcg
       61 cctcgccggg gtagaacaga atgcccgccg ggcgcgagat gacaaagccg tcatactccg
      121 aggtcagcac acccaagacc cgtccccacg catcgcgcat ctcaaccagc ggatcgccga
      181 ccttcacaag atcacccggt tctttgaggt gatgcaccat gcatgcctgc gtcacccgcg
      241 ccacactgcg tcgacgggtg gcatagccgg ggtccacgac tttgattccg gtgatggctt
      301 ccatctcacc gtccagcatt ccggaccagc gcattacatt gcgcaagcca gagactgctg
      361 catgaaccac ttccgggtat ggtgtatatg aactgcccaa ctccgccgtc aacgaaggga
      421 tgcccgccag catcgaaacc gaggcggtgg tagctcgatg caatttttcg tcaatgtatt
      481 tgcccagcgc aaaatccgcc gtcatggtgt gaccatacac ccgcgccatc tcgtccattt
      541 tttggctgac gatctcggcg cgtttgcggg cttcttcatc gcctttgcgg tacaagaccc
      601 ggtcacggaa gatgaacgag agcgcatgtc cacccataca gtgcaggtca aaataaaatg
      661 aagctgtatt cttgatttct tcaaacagcc gtccatatgc ctgctccaac gcagaaggtg
      721 gagttttatc tgggtctgtt tttggagtac gcccatcggg gaaaagccgg ttcgggtcac
      781 cgttgtgata gtacgcctga cgttccaacg tccgcagacc tgccgggttc aaggtaggga
      841 tggcaacgat cgtaccgcgt aattgcctaa ctaattcagg tgtaatcaat ttatgaatga
      901 cttgaatacc tgcgtgttca atgccgtgaa ttcccgccgt aatccaaaat acaggtccgt
      961 cctcatttcc ttgcgcgata atgacaggca aaacgtcagc gccacccact gggtgctgca
     1021 ccaaaaaata ctcgccgtac acgatctcgc cacgtttggc tacggcagtg ccgatggtaa
     1081 tagacatgaa aagtctcctc cattaactca cccccaaaca aaagaattta tttgatcatt
     1141 ccgtctgtaa gccactcagg gtctttggca ggcttccctg ccttccacgt ttctacgata
     1201 tgccgcagca tatccatttt gtgataacgc aacgcttcaa tgatctgagc atattgccga
     1261 tacgatgccc catcgccttc ttcagaactt aactccgaat ggacgatctt caataaagcc
     1321 aatgccagat ctgggttgat ctcatttgaa tcgaacaacg tctgaaataa attcagacga
     1381 tcggtggggg aaggttggcg aaacaagtgt ttataaagtg gagtaaagtt catgcgtccc
     1441 tgtattatac agtgaataaa agaattatcg gtacgatata atcctaaaca ggagcccaca
     1501 tgagccacat ttccccaccc gaatcacaag aatatgcccc tttttattcc gattacatcc
     1561 aacgagccag cgcgcgtggg gacttaaccg ccgctctatc gcttcaaccc gatgaaatga
     1621 aatctgccct gggcgggtta tctgactcac aagcccgctt caaacccgga cctcaagaat
     1681 ggtccatcaa agaagtcgta tctcatctga tagatggcga acgggtcttc tcctatcgtt
     1741 tgctgcgtgt ttcccgcaag gataaaactc ccctccccgg ctttgagcaa gatgactacg
     1801 tccgcgaagc caaagctgat gagatcgccc ttgcagatct gatcgatgaa tttacttttc
     1861 tacgccgcgc caacttgctt gccatcaaga acatgagcgc ctctgccctc gccgaagttg
     1921 gtaccgccag cggaaacccc gtcagcgccc gagccttggt ctatatactt gttggtcatg
     1981 tggagcatca catggctagc ctgaaagaaa agtatttacc ctttgcttaa tttttttcag
     2041 tgacagtcca tagaccaaag gctgtcactt tttgttctta aggacaattt catgcctacc
     2101 aacaaacacc accccactgc tcctaaacga ccacacccgt taaccagcca tggcgtcacc
     2161 cgcatggacg attattactg gatgcgcgac cgcaatgacc cagaaaccat gaaatacctc
     2221 aaagccgaaa gtgactatct tgaagaagtc atgcagcata ccaagtctac gcaggaagtg
     2281 ctcttcgccg aaatgaaggc aaggattcag gaaactgatt caaccgtccc ggagaaacga
     2341 ggcgagtatt tccactacga acgattcgaa aaggggaatc agtatccgat cttttgtcgt
     2401 aaaaaaggtt ctctcgaggc aaaagaag
//
LOCUS       DAOQJE010000597         2424 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_32019_length_2424_cov_5.517518, whole genome shotgun sequence.
ACCESSION   DAOQJE010000597 DAOQJE010000000
VERSION     DAOQJE010000597.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2424)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2424)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2424
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32019_length_2424_cov_5.517518"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..438
                     /locus_tag="PLT08_13935"
     CDS             <1..438
                     /locus_tag="PLT08_13935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase transmembrane
                     domain-containing protein"
                     /protein_id="HNE05622.1"
                     /translation="RLHEKLTGIMNKFLDGLASLRSPFNVLMVFFTSVVIWLLETAKY
                     WFVMHAFNFTVSFFALMLMNGIVNLATTIPSAPGYIGTFDAPGIAVLTAYGVDQATAA
                     GYTLVLHVALWVPITALGAYYLAREGIKWSDTLREEAETPNTP"
     gene            532..1143
                     /locus_tag="PLT08_13940"
     CDS             532..1143
                     /locus_tag="PLT08_13940"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNE05623.1"
                     /translation="MQPQLRKKRVLIADTTQETRRNTRVMLSTVKGVEVVAIALDGNQ
                     AVEMAKLHKPDIVLLDINMSKLNGLNAYKQILKVNPTAACILVSAENHPETVEAAKEM
                     GIQNFLVKPFTEHELEHAMQDVIQRLDGKLNNQRTIEVRKLADEYVRTKRTDEEAVQV
                     FEEAIQLPDSELVWMQTLAMIYVVRKRWGKLKVLAEQLELRTN"
     gene            1159..>2424
                     /locus_tag="PLT08_13945"
     CDS             1159..>2424
                     /locus_tag="PLT08_13945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HNE05624.1"
                     /translation="MKIAILGAGYGGMVAAWDLRKAGHDVTIFEAANYAGGLASGFKE
                     PHWDWSVEKFYHHWFQSDSEMLGLIRELGLEDKVIFPRPLTVMLYKNKWHPFDSILNA
                     LRFPGLGFGLNKIRFGFVGLFLRITKNWQALEKVTAHEWMLKYAGKQVYEQMWQPLLI
                     GKFASYYKDVNMAWLWARMHARTTRLGTFEGGFQKFADLFADKLREKGVEIRLGTSIK
                     SIKKDQASGSLSVDGESFDKVLVTTSPSLMAKLCPELPESYLKGLLELKSMGAVVMTL
                     SLKNSLSKEGYYWFNVPKEEGYPFLALVEHTNFVSKEHFGGDHIVYAGDYLELGHEYF
                     EMTDEQLLEKFIPAFEKFNPEFKRDWVKKIWVHKTNYAQPVPLVGHSKNIPAIQTPIE
                     GLYFASMSQVYPWDRGTNFAVEIGRKAARM"
BASE COUNT          631 a          573 c          607 g          613 t
ORIGIN      
        1 cgtttgcacg aaaagctgac cggtatcatg aataaatttc tggatgggct tgcttcgctt
       61 cgttcgccct tcaacgtgtt gatggtcttc ttcacttcgg tagtcatttg gcttttggaa
      121 accgccaagt attggttcgt gatgcacgcc tttaacttca cagtttcatt ttttgcactg
      181 atgctgatga acggcatcgt caacctcgcc accaccattc cttcggcacc gggctatatc
      241 ggcactttcg atgcgcccgg catcgccgtc ctcactgcct acggcgtgga ccaagccaca
      301 gcggcaggct ataccctcgt gttgcatgtg gcgttgtggg ttcccatcac cgcattgggt
      361 gcctattacc tggctcgcga gggcatcaag tggagcgaca cgcttcgcga ggaagcagaa
      421 acacccaaca ccccataaat ttacagctct gacaggcggg catcttcgga tgcccgcctc
      481 ggttatactt aataggttga accaattgcc ctattaagaa ggcaggacga tatgcagccc
      541 caacttagaa aaaaacgagt ccttatagca gatactacac aagaaacccg ccgtaacacg
      601 cgtgtaatgc tttctactgt caaaggtgtt gaggtggttg ccattgcact tgacggaaat
      661 caagccgttg aaatggcaaa gctacacaaa ccagatatcg tattgctcga cattaacatg
      721 tctaaactca atgggttgaa tgcttacaaa cagattctaa aggtcaaccc aactgcggcg
      781 tgcatcctcg tctcggctga aaatcatcct gaaactgtag aagccgccaa ggaaatgggc
      841 attcagaatt tcctcgtcaa accgtttacg gaacatgaac tggaacatgc catgcaagac
      901 gttatccaac ggctggatgg caagctcaac aatcagcgca ccatcgaagt cagaaagttg
      961 gcagatgaat acgtccgcac aaaacgaacg gacgaagaag ctgtgcaagt ttttgaagaa
     1021 gccattcaac ttcctgacag tgaattggtt tggatgcaaa cacttgctat gatctatgtt
     1081 gtacgcaaac gctggggcaa gttaaaggtc cttgcggagc aattggaact cagaacaaat
     1141 taaatttgga gaactctctt gaaaatcgca attcttggag cagggtacgg cggcatggtc
     1201 gccgcttggg acttacgtaa agcaggtcac gacgtcacca tttttgaagc agcaaattat
     1261 gcaggcggtc ttgccagcgg gttcaaagaa ccgcattggg attggtctgt ggaaaagttc
     1321 taccaccact ggtttcagtc tgatagcgaa atgttggggc tgattcgtga gctaggcttg
     1381 gaggataagg tcatcttccc gcgcccgttg accgtgatgc tgtataagaa caaatggcat
     1441 cccttcgact caattcttaa cgcgcttcgt tttcccggac ttggatttgg tctgaacaaa
     1501 attagatttg gcttcgtcgg gctttttctg cgcatcacca agaactggca ggcgctggaa
     1561 aaagtgaccg cccacgaatg gatgttgaag tatgctggca aacaagtcta cgaacagatg
     1621 tggcagcctt tactcattgg caagttcgct tcgtactaca aagatgtgaa catggcgtgg
     1681 ttatgggcgc gtatgcatgc ccgcaccact cggctcggca ccttcgaagg cggctttcaa
     1741 aaattcgctg acctctttgc agacaaactg cgcgaaaagg gagtggagat tcggcttgga
     1801 acaagcatca agtcaataaa gaaagatcaa gcgtcgggta gtttgagcgt ggacggtgaa
     1861 tccttcgaca aagtcctcgt caccacgtca cccagtttga tggcaaagtt atgccccgag
     1921 ttgcccgaat cttatttgaa gggtttgctg gagttgaagt cgatgggcgc agtggtgatg
     1981 actctttctt tgaaaaattc gctttcgaaa gaaggctatt actggttcaa cgtgcctaag
     2041 gaagagggct atcctttcct tgcactggtg gagcacacca actttgtttc gaaggagcat
     2101 ttcggcggcg accatattgt ttatgccggt gattaccttg aattgggtca cgaatatttc
     2161 gagatgactg atgagcaact gctggaaaaa tttattccag cctttgagaa gttcaaccct
     2221 gaatttaaac gcgattgggt caagaagatt tgggttcaca aaaccaatta cgctcaaccc
     2281 gtgcctttgg ttggacattc caaaaatatt cctgctattc aaacacccat tgaaggcttg
     2341 tacttcgctt cgatgagcca ggtctacccg tgggatcgtg gcacgaactt tgcggtggag
     2401 atcggtcgta aagctgcgag aatg
//
LOCUS       DAOQJE010000598         2424 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_32021_length_2424_cov_5.384550, whole genome shotgun sequence.
ACCESSION   DAOQJE010000598 DAOQJE010000000
VERSION     DAOQJE010000598.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2424)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2424)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2424
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32021_length_2424_cov_5.384550"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(147..1454)
                     /locus_tag="PLT08_13950"
     CDS             complement(147..1454)
                     /locus_tag="PLT08_13950"
                     /inference="COORDINATES: protein motif:HMM:NF016064.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeeE/YedE thiosulfate transporter family
                     protein"
                     /protein_id="HNE05625.1"
                     /translation="MNFPLSVLIVPSAEHPWTYLVFLVIGIAFGYTLEMSGFGDSRKL
                     AAQFYFTELTVLKVMFTAIVTAMVLLFGAVGLGFLDFNQVWVNPTYLSSGILGGLIMG
                     VGFIIGGFCPTTSLASASTGKIDGMFFMLGGFVGAFIFGETEKYFDAWYNYSGYYGRL
                     TLDQVFNLPIGVVVLLVILMALFMFWGAEQLERLIGKKDMSKEPRIRLAGAGALLVAA
                     IAVVAIGAPSLADRYNKLTFTRTETVEQLNADPIVNTYVYTADQMLEKRLVFITPAEA
                     YKAKYNQAMNPIYLDVRSEADYNLYHIEDAVNVPLERITEIVPMLLSEPPTNSVFIVM
                     SNDEAAAIQAWKLLSASSVSNVYILEGGVNNWIAFFGKDDTTFTAQPNPDAGDDQLAY
                     IFPSALGSRYESCSPNPVEYEALEFEAKIKLQLKRDKSGGGCG"
     gene            complement(1465..2010)
                     /locus_tag="PLT08_13955"
     CDS             complement(1465..2010)
                     /locus_tag="PLT08_13955"
                     /inference="COORDINATES: protein motif:HMM:NF016064.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeeE/YedE thiosulfate transporter family
                     protein"
                     /protein_id="HNE05626.1"
                     /translation="MTTQTKKSVFNRPEKPYVHPYFGGLVLGIILFLAFFLTGNGLGS
                     SGATSRIDAAIVDAISPSHVDNTPYLLKMAGGDKNPFDEWVVPVFFGALIGGFASGFF
                     NGRLKFMTTKGPNISDRTRWLMAFLGGVIFLYGARMARGCTSGQALSGGATLSAGSWV
                     VMFAIFGGAYGLAYFVRKLWN"
     gene            complement(2086..>2424)
                     /locus_tag="PLT08_13960"
     CDS             complement(2086..>2424)
                     /locus_tag="PLT08_13960"
                     /inference="COORDINATES: protein motif:HMM:NF013460.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b/b6 domain-containing protein"
                     /protein_id="HNE05627.1"
                     /translation="EKTPTRRMNPIQHATYFGILNVLLPLQMVTGALMWGVQKFPTFA
                     NALGGLPFLAPFHSLVAWLFATFILVHVYMSTTGATPLEATRAMITGYEEVEVHEVHE
                     EPKNEKKGKK"
BASE COUNT          678 a          569 c          612 g          565 t
ORIGIN      
        1 caagcaaacg tgaaatgttc atacgagtct ttcgggcgat ctaagaataa ttataactgc
       61 cgaatatatt ccaagtgtgc gcatagcatc aaaaaaacat ccctgaagta aatgcttcag
      121 ggatgtaata tttgcttgcg atgagcttat ccgcaacctc cgccagattt atcgcgtttc
      181 aattgcaact tgatcttggc ttcaaattca agggcttcat attccactgg gtttggcgag
      241 caagactcat agcggctgcc caaggcagag gggaagatat aggctaactg gtcatctcca
      301 gcatcggggt tgggttgagc cgtgaaagtc gtgtcatcct tgccaaagaa agcgatccag
      361 ttgttgactc cgccttccag aatgtagaca ttcgatacac tggatgcaga gagaagcttc
      421 caggcttgaa tggcagcggc ttcgtcattg ctcatgacga tgaagactga atttgtgggt
      481 ggctcagaga gcaacatagg tacaatctct gtgatacgct ctagtggcac gttgaccgca
      541 tcttcgatgt gatacaagtt gtaatctgct tcggaacgca cgtccaaata gatcgggttc
      601 atcgcctgat tgtattttgc tttataggct tcagcaggcg taatgaacac caaacgcttt
      661 tcaagcattt ggtcggcggt gtagacatag gtattgacaa tcggatctgc attcaattgc
      721 tcgactgttt cagtgcgagt gaaggttaac ttattgtagc ggtctgctaa tgacggagca
      781 ccaatggcaa cgacagcaat agctgctacg aggagtgcac cagctccagc aaggcggatc
      841 ctcggctctt tactcatatc ttttttgccg atcagtcgtt cgagctgttc tgcaccccag
      901 aacatgaaga gcgccatcaa aattacgagc aatacaacca cacctattgg caggttgaaa
      961 acctgatcta atgtgaggcg accgtaatag ccagagtagt tgtaccaagc atcgaaatat
     1021 ttttcggttt caccgaaaat aaatgcgcca acgaaaccac cgagcatgaa gaacatgcca
     1081 tcgatcttgc cagtggatgc ggatgcgagc gaggtagtgg ggcagaagcc gccaatgatg
     1141 aagccaacac ccatgatcaa accgccaaga atgccggagg aaagatacgt agggttgacc
     1201 catacttgat tgaaatccaa aaagccaagc ccaacggcac caaacagcaa gaccatggct
     1261 gtgacaatag cggtgaacat cactttgagc acggtcaatt cggtgaagta gaactgagcc
     1321 gccagtttgc gagagtcacc aaagccagac atttcaaggg tgtatccgaa ggcgatcccg
     1381 atcactaaaa agacgaggta ggtccaggga tgttcagcgc tcggtacgat aaggacggag
     1441 agagggaagt tcattgtatt ctccttagtt ccacaatttg cggacaaaat aagcgagccc
     1501 gtatgcgccg ccaaagatgg cgaacatcac gacccatgaa cccgctgaga gtgtagcgcc
     1561 gcccgatagt gcctgaccag aggtgcatcc gcgagccatg cgggcgccat ataagaagat
     1621 cacaccaccg agaaatgcca tcagccagcg tgtgcggtct gagatattgg gtcctttggt
     1681 ggtcatgaat ttcaaacgac cattgaaaaa gccagaggca aagccgccaa tcaatgcgcc
     1741 gaagaagacc ggtacaaccc attcatcgaa cggattcttg tcgccgccag ccatcttcaa
     1801 caaatagggc gtgttatcta catgcgatgg cgagatggca tctacgatcg ccgcatcgat
     1861 gcgcgaggtg gcacctgaag aaccaagtcc gttgccagtg agaaagaaag caaggaagag
     1921 gatgatccct aaaaccaagc caccaaaata agggtgaaca tagggctttt caggtcggtt
     1981 gaaaacagat tttttagttt gagttgtcat tgaaaactcc tataggtaaa gcatgaacct
     2041 gatccgggct gtgtacatca tgatatacac agcccgcgtt attctttact ttttgccttt
     2101 cttctcgttt ttcggttctt cgtgcacttc atgtacttcc acttcttcat agccagtgat
     2161 catggcgcgg gtcgcttcga gcggagtagc gccagtggtg ctcatgtaca catgcactag
     2221 gatgaaggta gcgaacaacc atgcaactag agaatggaac ggtgccaaga aaggcaaccc
     2281 accaagtgcg tttgcaaacg tcgggaattt ttgcactccc cacatcaagg caccagttac
     2341 catttggagc ggcaacaaaa cattgagaat gccgaagtag gttgcatgtt gaatggggtt
     2401 cattctgcgt gtgggtgtct tttc
//
LOCUS       DAOQJE010000599         2419 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_32139_length_2419_cov_5.799069, whole genome shotgun sequence.
ACCESSION   DAOQJE010000599 DAOQJE010000000
VERSION     DAOQJE010000599.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2419)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2419)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2419
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32139_length_2419_cov_5.799069"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2419)
                     /gene="nifJ"
                     /locus_tag="PLT08_13965"
     CDS             complement(<1..>2419)
                     /gene="nifJ"
                     /locus_tag="PLT08_13965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HNE05628.1"
                     /translation="VQEAQDFAAISQAATLKARVPFLHFFDGFRTSHEVNKIIQLSDE
                     ELRSMLDDDLIRAHRARGLSPEHPVLRGTAQNPDVYFQMREAANPYYAAVPAITQEMM
                     DKFAKITGRHYNLFDYSGDPNAERVIIIMGSGGEVVEETVNYLAQKGEKVGVLQIRLY
                     RPFSVEHFIKALPKSVKSLAVLDRTKEPGAMGEPLYMDVVSALVEGQRSNIKVIGGRY
                     GLSSKEFTPAMAKAVFDELTKPEPKNHFTVGINDDVSNTSLTVDSSFSIENEGMVSCV
                     FFGLGSDGTVGANKNSIKIIGEETDNFAQGYFYYDSKKSGTITMSHLRFGPKAIRAPY
                     LIEASQANFVACHQYSFLERVDMLKYAKEGGIFLLNSLYGPEEIWDQLQQEVQKDIIE
                     KKLKFYVINGYDVAERTGMGGRMNTIMQTAFFAISGILPKEQAIEQIKYAIKKTYGKR
                     GEAVVAKNFEAVDATVANIYEVKVPAKVSSKTVRRLPVPNEAPDFVKNVLGQIIDSDG
                     DNIPVSAFPVDGTFPTGTTQWEKRNLALEIPVWNEDICIQCGKCVLICPHAVIRHKVY
                     EDASLENAPETFKHTVSKFKEFSSGYSYTVQVAPEDCTGCSLCVEICPVKDKTAVGRK
                     AINMEPQPALREAEAKNWDFFMNIPDLDRKLINPSTIKNAQLLRPLFEFSGACAGCGE
                     TPYVKLVSQLFGDRAVIANATGCSSIYGGNLPTTPWAKNAEGRGPAWSNSLFEDNAEF
                     GLGMRLTIDKQKEYAEELLKSFEQTVGTQLVTDILHANQADEAGIDAQRVRVEELKSK
                     LAKSSDSR"
BASE COUNT          558 a          592 c          627 g          642 t
ORIGIN      
        1 gcggctgtcg cttgatttcg caagtttcga ttttagctct tccacgcgaa cgcgctgcgc
       61 atcaatgccg gcttcatctg cttggttggc atgcaaaata tcggttacca actgagtgcc
      121 taccgtctgc tcaaaggatt tgagcaattc ttcggcatat tctttttgct tatcaatggt
      181 gaggcgcatg cccaaaccaa actcagcgtt atcttcaaac aacgagtttg accaagcagg
      241 tccgcgacct tcggcattct tcgcccacgg agtggtgggc aagttaccgc catagatgga
      301 cgaacaacct gtggcattcg cgatcacggc gcggtcaccg aacaattgcg aaaccaactt
      361 gacgtacggg gtctcaccac agccggcaca agcaccgctg aactcgaaca acgggcgtag
      421 cagttgggcg ttcttgatgg ttgaagggtt gatcaacttg cggtcaagat cagggatgtt
      481 catgaagaaa tcccagttct tagcttcggc ttcgcgcaaa gcgggttgcg gttccatgtt
      541 gatcgccttg cgtccaaccg ccgttttatc cttcactggg cagatttcca cgcacaacga
      601 acagcctgta caatcttctg gcgcaacttg cactgtgtag gagtatccgc tcgaaaattc
      661 cttgaacttg gaaacagtat gcttgaacgt ttcgggggca ttttccaaac ttgcatcttc
      721 atacactttg tgacgaataa ctgcgtgcgg gcagatcaac acacacttac cgcattgaat
      781 acaaatatct tcattccaaa ccggaatttc caacgcgaga ttgcgctttt cccattgcgt
      841 agtgccggtg gggaaggtgc cgtctacggg gaaggcagag acggggatat tgtcaccgtc
      901 tgagtcgatg atctgaccga gtacattctt gacgaagtca ggcgcttcat tcgggaccgg
      961 gagtctgcgt acggtctttg agcttacctt cgctggaact ttgacctcat aaatatttgc
     1021 cacggtcgca tccacagctt caaagttctt tgccactacg gcttcgccgc gtttgccata
     1081 ggtcttcttg atggcatact tgatttgctc aatggcttgc tcttttggca aaataccgct
     1141 aatagcgaag aatgcagttt gcatgatggt gttcatgcgt ccgcccatac cggttctttc
     1201 cgctacgtca tagccgttga taacgtagaa cttgagcttc ttctcaataa tgtctttttg
     1261 tacttcctgt tggagctgat cccagatctc ttcgggacca taaagactgt tgagtaggaa
     1321 gatgccgcct tctttggcat acttgagcat atctacgcgt tcgaggaagg agtattgatg
     1381 gcaagctaca aagtttgcct ggctggcttc gatcagatac ggcgcacgaa tggctttggg
     1441 accaaagcgc aagtgtgaca tagtgatcgt gcccgatttc ttcgagtcat aatagaagta
     1501 gccttgggca aagttatccg tctcttcacc gatgatcttg atggagttct tgtttgcacc
     1561 tacagttccg tctgaaccga gaccgaagaa gacacaactg accatacctt cattttcgat
     1621 tgagaaggag gagtctactg tgagtgaagt gttggagaca tcgtcattga taccgacggt
     1681 gaaatgattc ttgggttcgg gcttggtaag ttcgtcaaaa actgcctttg ccatggcggg
     1741 agtaaactct ttggaagaca acccataccg accaccaatc actttgatgt tggagcgttg
     1801 accttctacc aaggcactga ccacgtccat gtagaggggt tcgcccatgg cgccaggttc
     1861 tttggtgcgg tctaacacgg cgagagattt cacgctcttg ggcaacgctt taatgaagtg
     1921 ctccacagag aatgggcggt ataagcgaat ttgcaagaca ccgacctttt cgcctttttg
     1981 cgcaagataa ttgacggtct cttcgactac ttcgccgcca gaacccatga tgataatgac
     2041 acgttcggca ttcgggtcgc cggagtaatc gaacaggtta tagtgacgcc cagtgatctt
     2101 ggcgaactta tccatcattt cttgcgtgat ggcagggacg gcagcgtagt aagggtttgc
     2161 cgcttcacgc atttggaagt agacatcggg gttctgggca gtaccgcgta agacgggatg
     2221 ctcagggctg agtccgcggg ctcgatgggc tcgaatcagg tcatcgtcca gcatgctccg
     2281 gagctcttca tccgataatt ggatgatctt gttcacttca tgcgaggttc taaatccatc
     2341 aaagaaatga aggaagggca cgcgtgcctt gagggtggcg gcttgagaga ttgcggcaaa
     2401 atcttgtgct tcttgcacg
//
LOCUS       DAOQJE010000600         2418 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_32159_length_2418_cov_4.253915, whole genome shotgun sequence.
ACCESSION   DAOQJE010000600 DAOQJE010000000
VERSION     DAOQJE010000600.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2418)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2418)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2418
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32159_length_2418_cov_4.253915"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1526
                     /locus_tag="PLT08_13970"
     CDS             <1..1526
                     /locus_tag="PLT08_13970"
                     /inference="COORDINATES: protein motif:HMM:NF013736.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HNE05629.1"
                     /translation="LILAIRGNFKYLFIFPLVTSFTLCIVSITDGKGSHDLIWVGPMG
                     LFLLANVYTRKNSIGPTLLGILSIALFVATGIAEISEVIPNRYGTNTEHILLGVLYLS
                     SIMGAITGIFHRHRNLLKIAIESKNEEAQIKKQYEEVISTLDQQVNARTVELDAINQE
                     LLSKTARLQAASDISQELTKIHYERNVDLLSYTTRLISEKLGYYHVGIFIVDKSRGFA
                     VLQAANSKGGQQMLTKHHQLKVGGTGIVGYVAQSGRPRIALDTGADAVFFNNPYLPET
                     RSEISLPIKVLSNVIGVLDVQSTQSAAFTNEDTNILMTIANQLALLLYEDESSTSTVS
                     TVSRKRTQFTDLDKQFGYSFRTDGSVVSNTAIDPSPAIKRAIASGETVAINSASKDGL
                     PMLAVPVKFREQVIGIIQVEASEKNRNWSEDEVLLVQAISDRAALALENARLFENATR
                     RAEQEETISRVTTQIGSSTDFDRIMQTTIQELGVALGASRSFIQIGSLSNSDEKASE"
     gene            1523..>2418
                     /locus_tag="PLT08_13975"
     CDS             1523..>2418
                     /locus_tag="PLT08_13975"
                     /inference="COORDINATES: protein motif:HMM:NF014763.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cache domain-containing protein"
                     /protein_id="HNE05630.1"
                     /translation="MSNFSNPSSPLKQTGQSETPNRKFSLGFKLPVAFIGLLLVALAL
                     YAFLSIRLSQQALVNTLRGELQDQVGTQVEVLQSNLLVAKAIASQLAAFAETEDFNES
                     DVPLLLQNTLKRNEHIYGSTIAYEPNAFRENLEYYAPYYYRSPDNVLEYADLGTPEYD
                     YFRKDWYTLPKAALTPIVTKPYFDYGGGNIWMVTWSIPFFSKNGEFKGVATADIAFLQ
                     TQEIVNKIKVGDNGYAFLIDSGGTILGIGENGGEYRVMSDSMVLAAYTEKTFRWIELV
                     SDMRAGKSGFIEATDPRGEPMLV"
BASE COUNT          704 a          534 c          509 g          671 t
ORIGIN      
        1 ctcttatatt agctattcga ggtaatttca aatatttatt tatcttccca cttgttacct
       61 cttttacgct ttgtattgta tctattacag atggaaaagg gtctcatgat ctgatttggg
      121 ttggaccaat ggggctgttc ctactagcca atgtctacac ccgaaaaaat tcaataggac
      181 cgacccttct tggcatacta agtattgcct tgtttgttgc aactggtatt gcagaaatat
      241 ccgaagtaat acctaaccga tatggcacta atacggaaca tattcttctg ggtgtcttat
      301 acctatcctc tatcatgggt gcaatcacag gtattttcca ccgtcatcgt aatcttctta
      361 agattgctat cgaaagcaaa aacgaagagg ctcaaatcaa gaaacaatat gaagaagtca
      421 tctccactct cgaccaacaa gttaatgcac gcacagtaga gttggatgcc atcaaccagg
      481 aattattaag caaaactgcg cggttacaag ctgcatcaga catttcgcaa gaattaacca
      541 aaatccatta tgaacgcaat gtagatttgc tctcgtatac tacacgattg atcagtgaga
      601 aacttggcta ttatcatgtt ggaattttta ttgttgacaa gtctcgtggc tttgctgttc
      661 ttcaggctgc aaatagtaaa ggtgggcagc aaatgctcac aaaacatcat caacttaaag
      721 tcggtggaac cggcattgta gggtatgttg cacaaagtgg gcgtccacgt atcgcattgg
      781 atacaggagc agatgcggtc ttcttcaata acccatatct gccagaaacc cgttctgaaa
      841 tatctctacc aatcaaggtt ttaagcaatg tcatcggcgt attagatgtt caaagcacac
      901 aatctgcggc atttaccaac gaagatacca atatcctcat gactattgcc aaccagctag
      961 cgctacttct ctatgaagat gaaagctcca cttccacggt ttctactgta tcgagaaagc
     1021 gcacccaatt cacagacctt gacaaacagt tcggatattc cttccgcacc gatggaagcg
     1081 tggtctcaaa cactgcaatt gatcccagcc cagcgattaa gcgagccatc gcctcaggcg
     1141 agactgttgc cattaacagc gcttcaaaag atgggttgcc catgttggct gttcctgtta
     1201 agttccgcga acaagtgatt ggcatcattc aagttgaagc ttcagagaaa aatagaaact
     1261 ggagcgaaga tgaagttctt ttggtgcaag ccatttcaga ccgtgctgcc ctggcattgg
     1321 aaaatgcccg tctgtttgag aatgccaccc gcagagcaga acaagaagaa acaatctccc
     1381 gtgttacaac ccaaatcggt tcgtcaactg attttgaccg catcatgcaa acgaccattc
     1441 aagaactcgg tgttgccctt ggggcatctc ggtcgtttat tcagataggt tctctttcga
     1501 attcagatga gaaggcgtca gaatgagcaa tttctccaat ccctcctccc cactgaaaca
     1561 gacggggcaa agtgaaacgc ccaatcgaaa atttagttta gggtttaagt tgccagtcgc
     1621 atttattggt ttgttattgg tcgcattggc gttgtatgcc ttcttaagca ttcggctttc
     1681 acaacaagca ctagttaaca cattgcgcgg cgaacttcaa gaccaagttg gcactcaagt
     1741 agaagtgctt cagtctaatc tattagttgc aaaagcgatt gcttctcaat tggcagcatt
     1801 tgcagaaaca gaagatttta acgagagtga tgtccctttg cttttgcaaa acaccttgaa
     1861 acgcaacgag cacatatatg ggtcaacaat tgcttatgag ccgaatgcgt ttagagaaaa
     1921 cctagaatat tatgctcctt actattatcg ctcgcccgat aatgttctag aatatgctga
     1981 tctaggaaca ccagagtacg attacttccg aaaagattgg tataccctac caaaagctgc
     2041 acttacccca atcgtaacca aaccctattt cgattatggc ggcggaaata tctggatggt
     2101 gacatggagt atcccgttct tttcaaagaa cggtgagttc aaaggtgtag ctactgctga
     2161 tattgcattt cttcaaacgc aagaaatcgt caacaagata aaagttggcg ataatggtta
     2221 tgcattctta atcgactctg gtggaactat tcttgggatt ggggaaaatg gtggtgaata
     2281 cagagtcatg tctgactcaa tggtattggc tgcctacacc gaaaaaacct tccgctggat
     2341 tgagttagtt tctgatatgc gagcaggcaa aagcggtttt attgaagcaa ctgacccgcg
     2401 cggtgaaccg atgttagt
//
LOCUS       DAOQJE010000601         2416 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_32210_length_2416_cov_6.846675, whole genome shotgun sequence.
ACCESSION   DAOQJE010000601 DAOQJE010000000
VERSION     DAOQJE010000601.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2416)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2416)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2416
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32210_length_2416_cov_6.846675"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..233)
                     /locus_tag="PLT08_13980"
     CDS             complement(<1..233)
                     /locus_tag="PLT08_13980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE05631.1"
                     /translation="MNQNNYKWGRLFFLVVALLTIFSLLLSACAPAQGLEGGAGGNGG
                     GNGGGNGGGGGNAGGSGNGGGGGNAGGSGNGGGG"
     gene            complement(288..755)
                     /locus_tag="PLT08_13985"
     CDS             complement(288..755)
                     /locus_tag="PLT08_13985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE05632.1"
                     /translation="MDQRNKSIQQDNELADFVDQLLDGKNPPASTSDEELVGLEKTLL
                     RLNDAFPPETLDEAKSKQMLARLKTRMRREEEEKAAKPSFWSRLFDLQANPQVGLLIA
                     AAVVVVFVIVSVPAVVQPSGSAVSGTASSGGSMYVGLGLLALLFIVYLVSRKK"
     gene            complement(758..1324)
                     /locus_tag="PLT08_13990"
     CDS             complement(758..1324)
                     /locus_tag="PLT08_13990"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HNE05633.1"
                     /translation="MTTNYMQNNLVSLQDASDELLIQKYLNGEREAFDALYHRHLPKV
                     YKRVRYVVPPNDVEDVTQEIFIAALKSLSSFRGDSKFGTWLRTLTNHKVAEFYRKRTR
                     KQEPQLAPLSDAIAQVAGSTSQSMEDRIFLQRALLNLPENYQEILLLRFAEDLQFNEI
                     AELTNQNLEATKSLFRRAVTALRNHLEN"
     gene            1486..2241
                     /locus_tag="PLT08_13995"
     CDS             1486..2241
                     /locus_tag="PLT08_13995"
                     /inference="COORDINATES: protein motif:HMM:NF013793.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MinD/ParA family protein"
                     /protein_id="HNE05634.1"
                     /translation="MSKIISVHSFRGGTGKSNTTANIAALLAMDGARVGVVDTDIASP
                     GIHVLFNLDEADMENSLNDYLWGKCTIEQAAQDVTGHIGAEVKGQIFLIPSSIKAGEI
                     ARILREGYDVGLLNDGFRDVIEKLKLDYLLIDTHPGLNEETLLSIAISNALVIIMRPD
                     QQDYQGTAVTVDVAKKLDVPNLFILVNKAPQNLDLEEVRARVEQTYDAKVGAILPHSD
                     EMMMLASSGLFTVHYPEHVVTKGLRKLVDQLKA"
BASE COUNT          608 a          588 c          590 g          630 t
ORIGIN      
        1 cccccgccac catttccact accaccagcg ttaccgcccc cgccaccatt tccactacca
       61 ccagcgttac cgcccccgcc accatttccg ccaccatttc cgccgccatt gccaccagcg
      121 ccgccttcta atccctgagc aggggcacaa gcagataaaa gcagtgaaaa gatcgtcaac
      181 aaagcgacga caagaaaaaa caatctgccc catttgtaat tattttgatt cattgatctc
      241 tccttgatac gaataaaaat atcggtttgg tttgtcttga atatttatta tttctttcga
      301 gaaaccaaat agacaatgaa cagcaaggct agcaatccca aaccgacata catactgcca
      361 ccggaagatg ctgttccaga gacggcagaa cctgagggtt gaaccacagc aggaacacta
      421 acgatcacaa aaacaaccac tactgctgct gcgatcaaca aaccgacttg ggggttggct
      481 tgaagatcaa aaagcctgct ccaaaaggaa ggcttggctg ctttttcctc ttcttcccgg
      541 cgcatacgag tctttaggcg ggcaagcatt tgcttggatt ttgcttcgtc cagggtttct
      601 ggagggaagg catcgttcaa acggagaagc gttttctcga ggccgaccag ttcctcgtct
      661 gaggttgagg cgggagggtt tttcccatcc aacaactggt cgacaaagtc cgcgagttcg
      721 ttatcttgtt gtatagattt gtttctttga tccatggtta attttccagg tggttgcgaa
      781 gtgcggtaac ggctcgacga aacaaagatt ttgtagcttc aagattttgg ttggtcaatt
      841 cagcaatctc gttgaactgt agatcttcgg caaaacgcag cagcaagatt tcctgatagt
      901 tctctggcag gtttaggagc gctcgttgga ggaagatgcg atcttccatc gattgggacg
      961 tgctaccagc gacttgggca attgcgtctg acaatggtgc aagttgcggc tcctgcttgc
     1021 gggtgcgttt tcgatagaac tctgccactt tgtggttggt aagggtgcgc aaccatgtgc
     1081 caaatttcga gtcacctcga aatgaggaaa gggactttaa cgcggcgata aagatctcct
     1141 gcgtcacatc ctctacatcg ttgggcggaa ccacatacct gacccgtttg tagaccttgg
     1201 ggaggtggcg gtgataaaga gcatcgaaag cttctcgttc tccattaagg tatttctgga
     1261 tgagaagttc gtcgctggcg tcctgcaggg atactaggtt gttttgcata taatttgtcg
     1321 tcatgaagcg ccaaaatggg tcgttttcgt ctttcagttt agaaaggtac tataacataa
     1381 ctgagaaatg aaaagtaggg atttgatttt caatatagtt aggctataat tatcgaactg
     1441 gttttcagag atttcgaaag actatcatag aatcggagac agtgcatgtc caaaatcatt
     1501 tcagttcatt ccttccgcgg cggcacgggt aaatcgaata ccacagcaaa tattgctgct
     1561 ttgctcgcca tggatggtgc acgtgttggt gtagtggata ctgacattgc ctcgcccggt
     1621 attcatgttc tcttcaattt agatgaagcg gatatggaaa actccctcaa tgactatttg
     1681 tggggtaagt gcaccatcga acaagccgct caagacgtga ctggacatat cggcgccgag
     1741 gttaagggtc agattttcct catcccctcc agcatcaaag caggcgaaat cgcccgcatt
     1801 ttgcgcgaag gttacgacgt gggtctgttg aacgacggct tccgcgatgt gatcgaaaag
     1861 ttgaagctgg attatcttct tattgatact caccccggct taaacgaaga gaccttgctc
     1921 tccattgcca tctccaacgc cttggtcatc atcatgcgcc cagaccagca agattatcag
     1981 ggcacagcag tgaccgtgga tgttgcgaag aagctcgatg ttcccaacct ctttattctc
     2041 gtcaacaaag cgcctcagaa tctggacctt gaagaggtcc gcgcccgcgt ggagcagacc
     2101 tacgacgcga aagtgggagc tattctcccg cactcagatg aaatgatgat gctcgccagt
     2161 tctggtctct tcaccgttca ttatcctgaa catgtggtga ccaaaggctt gcgcaaactg
     2221 gtggatcaac tcaaagccta gggcattcgc ctaggtgaag ggaaggcgcc atgagcaatc
     2281 aactttttga agctgcgctt aaggaattgg aagcacgtca ggaagatgtt cgcccttcag
     2341 acgtgatatc cttctctgag cccctgcgtt cggctgtaaa tttcgttgtg cggctgaagc
     2401 gttttagtct gacaga
//
LOCUS       DAOQJE010000602         2415 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_32235_length_2415_cov_6.384322, whole genome shotgun sequence.
ACCESSION   DAOQJE010000602 DAOQJE010000000
VERSION     DAOQJE010000602.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2415)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2415)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2415
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32235_length_2415_cov_6.384322"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..731)
                     /locus_tag="PLT08_14000"
     CDS             complement(<1..731)
                     /locus_tag="PLT08_14000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE05635.1"
                     /translation="MLKKYFIFLSTHVNAILNRLRTKIPGLPSNRVLLSTAGVVVAAG
                     GIFVALQVLVAPDQPVTTYTLPYTQTFDDVNLKRWFSQSGVWTIRTGTLMQTVGGEEA
                     GQMHIPLKVPEDAPYHASVFITLKKDTKQVGLSFNAQYPDLIAKQHRVYISRINPETL
                     ELVAGYMDETGAFITQAQVPLTLNTTEFRLDVYVYSNSYLVQVNGQRMIENRPLFYQN
                     GMIGFYALGSAVFDTYKLTTAENQNP"
     gene            complement(854..>2415)
                     /locus_tag="PLT08_14005"
     CDS             complement(854..>2415)
                     /locus_tag="PLT08_14005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE05636.1"
                     /translation="VWLHDVFMQTTYPKLVWILTGKHLPAEKKSRRQNQVAPKKEAGR
                     FIPLLMAVLFMFPLFSNQSYRIVFSSGDFNQFLTPYPVGPLKEVKNALLKLPPGKVVV
                     LPPTETAKVVIDINGVEHKFIDKFHIYYLDLPSYYYGLTGDSDNKHEFFLLLRALYYQ
                     QPWWINIARDLDLKYIVVNKELVASTVGGQEYLKEVEKILIPELDSRDAYVKKVMENE
                     SYVLYEFTDLPTAERVPLYLDVDWNSFIRILSSNLELTRYYDLRHTMVVGDLEDFDSL
                     TMVTDDEHESALDLYEKKNKKQFFRPSSVILPFDPEQISSSYYLSPMFRLFQFFSDSK
                     YNRLDMITPGLWGTLEGGFIGVPREAPFRIDVTLPEEGEYRLLMRGAISAVDMEMESK
                     LFPEPQRISLASDPSNLAFFDKRLVFSSSRTPLDVSQYTYQELGMLIPTDVVVVNYQY
                     QFFDLGVVQASKGKYPIYFNKLNDAPMLVEGILVIPEEVYQTLTLPSNVTVVQPNDLC
                     CGSVIIQGEEP"
BASE COUNT          711 a          608 c          476 g          620 t
ORIGIN      
        1 gggttttggt tttcagcagt agtgagttta tacgtatcaa aaactgccga accaagcgcg
       61 taaaaaccga tcatgccgtt ctggtaaaac agcggacggt tctcaatcat gcgctgaccg
      121 ttgacctgca ccagatacga attgctataa acatacacat ccaaccgaaa ttcggtggtg
      181 ttgagcgtca gcggaacctg cgcctgtgtg atgaatgccc ctgtttcatc catatacccg
      241 gcaaccaatt caagcgtttc agggttgatg cggctgatat acacccgatg ttgttttgcg
      301 attaagtcag gatattgagc attgaacgaa agccctactt gttttgtatc ctttttcaat
      361 gtgatgaata cgctggcatg atacggcgca tcttctggca ccttgagcgg aatatgcatc
      421 tgccccgctt cttctccgcc aactgtttgc atcaacgtcc ccgtacggat cgtccacaca
      481 ccagattgag aaaaccagcg tttgagattc acatcatcaa acgtctgtgt gtatggcaag
      541 gtgtaagtcg tcacaggttg gtcaggcgca accagtacct gcaatgccac aaaaatcccg
      601 ccagcagcca ccaccacacc ggcagtggat aacaatacgc gattcgaggg cagaccaggg
      661 atctttgtcc gcagtctgtt caaaatagca ttcacatgag tagaaaggaa aataaaatac
      721 ttcttcagca taataattaa ttccattcta gtgaaaatag aaaaatacta catttcagac
      781 tcgttttcaa aaaactaaag attttgcaaa tcaataaata caggttttcc cccattgtta
      841 ttttgcgtta actctaaggc tcttcccctt gaataatgac cgaaccacag cacaggtcat
      901 tgggttgcac caccgtaaca ttggaaggca atgtaagggt ctggtaaacc tcttcaggaa
      961 tcactaaaat gccttccact aacataggtg catcattgag cttattgaaa tagatcgggt
     1021 atttcccttt ggatgcctgt acaacaccca agtcaaagaa ctgatattgg taatttacaa
     1081 ccaccacatc ggttgggata agcattccca attcttggta ggtatattgg ctcacatcca
     1141 acggagtgcg gcttgaagaa aagactaaac gcttgtcgaa aaatgccaaa tttgatgggt
     1201 ctgaagcaag cgaaatccgc tgcggttccg ggaaaagctt cgactccatt tccatatcca
     1261 ctgccgagat cgcaccacgc atcagcaggc gatattcacc ctcttcaggc aatgtcacat
     1321 cgatgcgaaa tggagcttca cgcggcactc caatgaaacc gccctccaac gtgccccaca
     1381 agcccggcgt gatcatatcc aagcgattgt atttactatc cgagaagaac tgaaacaaac
     1441 gaaacatggg cgagagataa tatgaacttg aaatctgctc tgggtcgaat ggcaaaatca
     1501 cactactcgg acggaaaaat tgcttcttat ttttcttctc atacaagtcc agcgctgatt
     1561 cgtgttcatc atccgtcacc atcgtcagcg agtcaaaatc ttccaaatcc cccaccacca
     1621 tggtatgacg caggtcataa taacgagtca gttccaaatt agaagacaga atccgaatga
     1681 atgaattcca atccacatcc aaatacaaag gcacgcgttc cgctgtgggc aggtcagtaa
     1741 attcatacag cacataactt tcgttttcca tcaccttctt aacataggca tcacgtgaat
     1801 ccaattcagg aatcaaaatc ttttccactt ctttcaaata ttcctgtccg cccacagtgc
     1861 ttgccaccaa ctctttatta accacaatgt atttcaaatc caaatcacgg gcaatattga
     1921 tccaccaagg ttgttgataa tacaaagccc gtaaaagtaa aaagaactca tgcttgttat
     1981 ccgagtcgcc cgtcaaaccg tagtaataac tgggcaagtc aaggtaataa atatgaaact
     2041 tatcaatgaa cttatgctcc accccgttga tatcaattac taccttcgcc gtttctgttg
     2101 gcggtagcac cactactttc cctggcggca acttcaacag agcgttcttt acttccttca
     2161 acggtccaac cgggtatggc gtcagaaatt ggttgaaatc tccagagcta aacacaatac
     2221 gatatgactg attggagaac agcgggaaca taaacagtac cgccatcaac agcgggataa
     2281 aacgccccgc ctctttcttc ggcgcaacct gattctgcct tctcgatttt ttctcagccg
     2341 gaagatgttt tccggttaat atccacacca gctttgggta cgtagtttgc ataaagacat
     2401 catgcagcca tacca
//
LOCUS       DAOQJE010000603         2413 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_32283_length_2413_cov_5.510602, whole genome shotgun sequence.
ACCESSION   DAOQJE010000603 DAOQJE010000000
VERSION     DAOQJE010000603.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2413)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2413)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2413
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32283_length_2413_cov_5.510602"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(171..2072)
                     /locus_tag="PLT08_14010"
     CDS             complement(171..2072)
                     /locus_tag="PLT08_14010"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF017076.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016849 - phosphorus-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0032555 - purine ribonucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HNE05637.1"
                     /translation="MSKNNNPLNLRLILVLGTAALLLIVQVLGLFSTITTPIERIEYS
                     MQDTFVRLRGAEKPSGEIVIVAIDDQSFNWTGLQWPWPRSYFAEIVDQINKGGGKVVG
                     MDLILFEPDEKQPENDLVLAKALGESPAAVAVMQINKTGGEGYATETLSQPLSAYRDV
                     LDGIGTTDVKRDEDAIVRNVSAYSIYQGQVYPHWAFELAKLYLNAEQPIFGNSSVKFN
                     GQNIPLRSNELPIYFAGPAGTYPTYSASDVHDGITLEQNPDAFRGKIVLIGATTITLQ
                     DIYPTPFSATDPTPGVEVVANTVDTIINGKFITYAPPWLALLLIIVAAGMAYLITLSK
                     RPSFTLTALGISILLYLVAAYFVFSSQRYILPTIAPTLMLFLGVILPTLEQAVSQEQE
                     KRRVRNLFSRFISPEMVDQMMKTQDINSLNKRADLSIIFSDIRGFTTLSEKMTPEGVV
                     ALLNPYLEAMSQVIYKHGGTVDKYEGDAIIAFFGEPVPYKDHAVRALRASMDMRKALA
                     ELRDKWAKEGRPSQIEMGVGINSGEVFVGLLGSAQRINYTVIGDNANLASRLQDLTKN
                     YAWPILISESTYQQVKDEFDCELADAVTVKGKTKAVNVYKVIGEKGAPESEQLQAWTS
                     DQVKPKHTS"
     gene            complement(2100..>2413)
                     /locus_tag="PLT08_14015"
     CDS             complement(2100..>2413)
                     /locus_tag="PLT08_14015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE05638.1"
                     /translation="EDGSTARIETSSNSAEFYIEVLPAGGNYQWFVTAYGADGKEICK
                     SNSANFSKPQAEPTEKPKATKDPNAEEPASGTAGCPCDPYDYLNYSDCVGSHMDVCSI
                     P"
BASE COUNT          617 a          536 c          583 g          677 t
ORIGIN      
        1 actataccaa aaaaacgctt tttgtacaat tatgtacaag tctttcacaa ataaacaggg
       61 gattaaaaat ccgcctccag cgtggatttc tgcctggttg acagaccaag cctgcgtcta
      121 cgcctgagcc ggtacaaaaa cggagcgaga attctcgctc cgtttttgtg tcatgatgtg
      181 tgtttgggct tcacctggtc gcttgtccag gcttgtagtt gttcgctttc tggagcgcct
      241 ttttcgccaa tgaccttgta tacattgacc gccttggttt tccctttgac ggtgactgca
      301 tctgcaagtt cgcaatcgaa ttcatccttg acttgttgat aggtgctttc actgatgaga
      361 atgggccagg cgtagttttt ggtgaggtct tgcaggcgag aggcaaggtt ggcattatcg
      421 ccaatgaccg tatagttgat gcgttgcgct gagccaagca gccctacaaa cacctcgccc
      481 gagttgatgc ctactcccat ttcgatctgg ctggggcgac cttcttttgc ccatttatct
      541 cgaagctcag ccaatgcttt gcgcatatcc attgaggcgc gcagagcacg aaccgcgtgg
      601 tctttatagg gaacgggttc gccaaagaac gcaatgatgg catcgccttc gtatttatct
      661 acggttccgc catgtttata gatgacctgc gacatggctt cgaggtaggg gttaagcagg
      721 gcaaccactc cttccggcgt cattttttca gaaagtgtcg tgaagccgcg aatgtctgaa
      781 aagataatgg aaagatctgc acgtttgttg agtgaattaa tatcttgagt cttcatcatc
      841 tggtcaacca tttctggaga aatgaaacgg ctgaatagat tacgaacacg gcgcttttcc
      901 tgttcctgag aaactgcctg ctctaatgtg ggaaggataa cgccaaggaa aagcataaga
      961 gttggcgcaa tggttggcaa tatatagcgt tgactggaga aaacaaaata cgcagcaacc
     1021 aaatagagaa gaatgctaat gcctaaagcg gtgagtgtaa aagacgggcg ctttgaaagc
     1081 gtgataaggt atgccatacc agccgccacg attattagca aaagtgccaa ccaaggtggg
     1141 gcataggtaa tgaacttgcc attgatgatt gtatctaccg tgttcgcaac gacttccaca
     1201 ccaggtgttg gatctgtcgc cgaaaagggg gttgggtaaa tatcctgtaa tgtaatcgtc
     1261 gtcgcgccaa tcaagacaat tttgccccgg aaggcatctg ggttctgttc caacgtgatg
     1321 ccatcgtgaa cgtctgatgc agaataggtt gggtaggttc cggcaggtcc tgcaaaatag
     1381 ataggaagtt cattggaccg taacggaatg ttttgaccgt taaatttaac gcttgaattt
     1441 ccaaatattg gttgttcagc attcaaatat aactttgcaa gttcaaatgc ccaatgcgga
     1501 taaacctgtc cctgatagat actgtatgcc gatacatttc gcacgatggc gtcttcatcg
     1561 cgttttacat ctgttgtgcc tatgccatcc agaacatcac ggtacgcaga taatggctga
     1621 gacagggttt cggttgcata gccttctcca cccgttttat tgatctgcat cacagcaact
     1681 gcggcggggc tctctccgag tgcttttgcg agaaccaggt cattttcagg ttgcttttca
     1741 tcgggctcga aaaggattaa atccatacca acgaccttgc cgccgccttt attgatttgg
     1801 tcaacgatct ctgcaaaata ggagcgaggc caaggccatt gcaagccggt ccagttaaaa
     1861 gattggtcgt caattgctac aatgacaatc tcaccagatg gtttttccgc tccacgcagg
     1921 cgcacaaatg tatcctgcat ggagtattca atccgctcaa tgggtgtggt gatggtgcta
     1981 aataatccca gaacttgaac aattaagagt agtgcagctg tcccaagtac cagaataagg
     2041 cgtaagttta agggattatt attttttgac atgtcttatc ctttgatagc ttgagatgat
     2101 taaggaatac tacaaacatc catgtgagag ccaacacaat cggaatagtt caagtaatca
     2161 taggggtcac aaggacagcc agcggtacca ctagcaggct cttctgcatt tggatctttg
     2221 gttgcttttg gcttttcagt tggttctgct tgcggttttg agaaattggc actatttgat
     2281 ttacaaattt cttttccgtc tgcgccgtag gctgtgacaa accattgata gtttccacca
     2341 gcaggcaaaa cctcaatgta aaattctgcg ctatttgaac tggtttcaat tcgagcggtg
     2401 ctgccatctt ctt
//
LOCUS       DAOQJE010000604         2411 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_32339_length_2411_cov_6.039049, whole genome shotgun sequence.
ACCESSION   DAOQJE010000604 DAOQJE010000000
VERSION     DAOQJE010000604.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2411)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2411)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2411
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32339_length_2411_cov_6.039049"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1092
                     /locus_tag="PLT08_14020"
     CDS             <1..1092
                     /locus_tag="PLT08_14020"
                     /inference="COORDINATES: protein motif:HMM:NF014667.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCO family protein"
                     /protein_id="HNE05639.1"
                     /translation="PYEILCGVLMKRGILLLIGILLLFSALIAGYILTQTYNFNGYVQ
                     PEPIKAPETMLRSANGPVRLSDFQGKIVLLYFGYTFCPDVCPTSLANIKLALADLTPE
                     EAAQVQVIFVSVDPARDTPEALGQYVNMFQDNFIGATGTRDEIDWMVNAFGAKYTIEP
                     PDANGNYNVSHSAFTMVIDRNGYYVLNWAHGTPSTDMSADLHHIINEGIPVSAQILAG
                     PTYTPVVCAVTLIPSHVQNGQWLYEHHCAQCHGMDLAGNPQWQVELADGSHLAPPLNG
                     SGSAWKYSRTDLLAIIKEGRNLEKPIHMPAFKSNLADWEIDFILTYIQSTWDINQLNY
                     QHGFMTLTPNPTSTPAAPLAPLVTVTPSP"
     gene            1173..1445
                     /locus_tag="PLT08_14025"
     CDS             1173..1445
                     /locus_tag="PLT08_14025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE05640.1"
                     /translation="MKNGTCPKCGSNEILSELPLHGGEGHPSYVDIVQPEPPNRPFIW
                     VAQSEQSKFVAYICGACGYSEFYAVRHQALNEGRKKGFKPRFDYTA"
     gene            1512..1862
                     /locus_tag="PLT08_14030"
     CDS             1512..1862
                     /locus_tag="PLT08_14030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE05641.1"
                     /translation="MKVISIFLALINFLTGVLLILSCISANETFAWVAWKTGLGVLGV
                     TFGILTFKDSAQPVSQRKMILYGLLLVVVGVSIVAYGIHWSVISGDPKHTVMVVGSSF
                     FLHGFTSVLGMASD"
     gene            1951..>2411
                     /locus_tag="PLT08_14035"
     CDS             1951..>2411
                     /locus_tag="PLT08_14035"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNE05642.1"
                     /translation="MFTRLEKLYHQFPPRYWVIVLTFFIDSIGGTLLFPFFSLYITQK
                     FNVGMTQAGMVLAMFSIFGIVGNMIGGALTDRLGRRKLILFGLVFSALSTLSLGLVNS
                     LAVLFPLAAFIGLLSDIAGPANQAMMADILDEKQRQEGFGLMRVIRNLAWI"
BASE COUNT          556 a          687 c          545 g          623 t
ORIGIN      
        1 ccctacgaaa tcttgtgcgg agttcttatg aaacgtggca tccttctctt aatcggcatc
       61 ttgttattat tctcagccct tatcgctggc tatatcctca cccaaaccta caacttcaac
      121 ggctatgttc agcccgaacc gatcaaagca cctgagacca tgttacgctc cgcaaatgga
      181 ccggtccgct tgagcgattt tcagggcaag attgtgttac tgtatttcgg ctacaccttc
      241 tgcccagatg tttgccccac ctcgttggcg aacatcaagc tggcgctcgc agatttgacc
      301 ccggaagaag cggctcaggt gcaggtcata ttcgtttctg tagacccagc tcgcgacacg
      361 cccgaagcgc tcggtcagta tgtcaacatg tttcaggata acttcatcgg cgccaccggc
      421 acgcgtgacg agatcgattg gatggtgaat gccttcggcg ccaagtacac catcgagccg
      481 cccgatgcca atggcaacta caacgtcagt cactcggcgt tcacgatggt gatcgaccgt
      541 aacggctatt acgtgttgaa ttgggcgcat ggcacaccgt ctacagatat gagcgcagac
      601 ctgcatcaca tcatcaacga aggcattccg gtcagtgcgc agattttggc aggtccaacc
      661 tatacgccag tcgtttgtgc ggtcacgttg attccgtcgc acgtccaaaa cggtcaatgg
      721 ctgtatgaac atcattgcgc acaatgtcac ggcatggact tggctggcaa cccacagtgg
      781 caggtagagt tggcggatgg ttcgcatctg gctcccccgc tcaacggcag tggaagcgcg
      841 tggaagtatt ctcgaaccga cctgcttgcc atcatcaaag aaggacgcaa tctcgagaag
      901 cccattcaca tgccagcctt caaatcaaac ctcgccgatt gggagatcga cttcatcctc
      961 acctatattc aaagcacttg ggatattaac caactgaact atcaacacgg attcatgacc
     1021 ctcactccca acccaaccag cacccctgcc gcgcctcttg ccccgctcgt aacggtaacg
     1081 ccctctccat aattttctcc accctttggc ggatactcct ttacaaaccc acaggctatg
     1141 atgagtgcat ctcatccttt aggagcttca tcatgaaaaa cggaacttgc cccaaatgtg
     1201 gctcgaacga aatcttaagt gaacttccct tacatggcgg cgaagggcat ccatcatacg
     1261 tggatatcgt ccaaccagag ccgccaaacc gtcccttcat ttgggtggcg caaagcgaac
     1321 aaagtaaatt tgtggcatat atttgtggtg cttgcggcta ttctgaattt tatgccgttc
     1381 gccatcaagc tctaaacgaa ggtcgaaaaa aaggcttcaa accgcgcttc gattacactg
     1441 cctgacagac tctcaatagt ctttgacagc cctaccctgc tatcctgacc tcaacacaag
     1501 gaggcacggg tatgaaagtc atctctattt ttctggcgct aatcaacttc ctcacagggg
     1561 ttttgttaat tctctcctgc atctctgcca atgaaacctt cgcctgggtc gcttggaaaa
     1621 ctggcttggg cgttctgggc gtcacctttg gcattctcac tttcaaagat agcgcacagc
     1681 ctgtcagtca acgcaagatg attttgtatg gcttgctctt ggtcgtggtc ggcgtttcca
     1741 tcgtggcgta tggcattcac tggtctgtca tcagcggtga cccaaagcat accgtcatgg
     1801 tggttggcag tagtttcttt ctacacgggt tcacctcggt cttaggcatg gcaagcgatt
     1861 aatctcaccg cataggtctt cccgctctgc tctctgtagc aaaagaaaaa tctctttcct
     1921 aattcagaac aaacgtgcta ttattgcgag atgttcacac gtctcgaaaa actttatcac
     1981 caatttcctc cccgctactg ggtcatcgtc ctcaccttct ttattgatag catcggcggg
     2041 acgctgctct tccccttctt ctctttgtat attacccaaa aattcaacgt cggcatgacg
     2101 caagctggca tggtacttgc catgttttcc atcttcggca tcgtcggcaa tatgattggc
     2161 ggcgccctca cagaccgact tggtcgccgg aagttgattc tcttcggctt ggtctttagc
     2221 gcactgagca cactgtcgct cgggttggtc aactcgctag cagtcttatt tccgctcgcc
     2281 gcatttatcg gtctgctttc agatattgcc ggtccagcca accaagccat gatggcagat
     2341 atcctcgacg aaaagcaacg tcaagaagga ttcggactga tgcgcgtcat ccgcaacctg
     2401 gcatggatta t
//
LOCUS       DAOQJE010000605         2411 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_32350_length_2411_cov_3.561545, whole genome shotgun sequence.
ACCESSION   DAOQJE010000605 DAOQJE010000000
VERSION     DAOQJE010000605.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2411)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2411)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2411
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32350_length_2411_cov_3.561545"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            49..1233
                     /locus_tag="PLT08_14040"
     CDS             49..1233
                     /locus_tag="PLT08_14040"
                     /inference="COORDINATES: protein motif:HMM:NF013740.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HNE05643.1"
                     /translation="MKKTNDQWSLPFRYTMGIIIFILVVALLIYAREAVTMLVIAGFI
                     AYLISPAVIFLKDRTGWSRKVAVNVVYFTALIVLVGIPATLTPIFFDEIQLVAQDVLE
                     LSQDFSNFLSKPIQVGNVVVHLEQLGESLAHIQDSILTPLPEEALRLLEATSINTLWV
                     LIILVAVHLFMSEWPKIHSWLVHLAPEPYQDDMRELYNRLRSVWMAYLRGQIVLMLVV
                     GVTFTIAWMIIGIPGALVLGMLAGLFTLVPDVGPILAAVIAVCVALLEGSTRLYFSDD
                     VIMNNLIIAGITFATYLVLINLKNFFVRPIIMGRSLHMNEALIFIAILTATILWGILG
                     ALLIVPVMASISVIMEYLRRRLLGLPPFDGDGLKQFKAPPEKVQTRSLKIPTKDKQKK
                     QK"
     gene            1230..2159
                     /locus_tag="PLT08_14045"
     CDS             1230..2159
                     /locus_tag="PLT08_14045"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HNE05644.1"
                     /translation="MKITYSIIAPIYNEIDNLSELYRRVKEVMDANGEPWEFILVDDG
                     STDGSTDKIRELAEKDKTIRPVIFARNFGHQVAITAGWDYARGDAVVIIDADLQDPPE
                     VILELAKKWKEGYEVVYAVRGEREGESWFKLWTASMFYRLIYRITDVKIPVDTGDFRL
                     MDRKVVNVLKQMKERHRFPRGMSAWVGFKQVGVTYKRAARHAGITKYPFRKMLRLAVN
                     AITGFSYFPLQVATYIGFISAGISIFAIPVVVIMRMAGSGAFFGQATTLIAVLFLGGV
                     QLISLGILGEYVGRLYDEAKGRPLYIVRDAPED"
BASE COUNT          572 a          487 c          598 g          754 t
ORIGIN      
        1 atctgcgccg atcattgaac caactttaga agcggtgaaa aagattttat gaaaaaaaca
       61 aatgaccagt ggagtcttcc ctttcggtat acgatgggga tcatcatttt tatcctcgtt
      121 gtggcattgt tgatctatgc ccgcgaggct gtgactatgt tggtgatcgc tgggtttatt
      181 gcgtatttga tcagcccggc ggttattttt ctaaaagata gaactgggtg gtctcgaaaa
      241 gtagcagtca acgtggtgta tttcacagca ttgatcgtac ttgtgggtat tccagctaca
      301 ttgacaccaa ttttctttga cgaaattcaa ctggtagcgc aggatgttct ggaactctcg
      361 caggatttta gcaatttcct ttccaagcca attcaagtgg gtaatgtggt tgtacatttg
      421 gaacaattgg gagaaagcct tgcccatatt caagatagta ttctgacgcc tctccctgaa
      481 gaagcgcttc gcttgctaga ggctacttcg ataaatactt tgtgggtgtt gattatcttg
      541 gtggctgtgc atttgtttat gtctgaatgg cccaagattc attcatggct ggtccatttg
      601 gcgcctgaac cttatcagga cgatatgcgt gagctgtata accgtttgcg tagtgtgtgg
      661 atggcatatc tgcgcggaca gatcgttttg atgttagtgg ttggggtgac tttcaccatt
      721 gcctggatga ttattggtat tccaggtgcg ttggtattgg gtatgttggc agggttattt
      781 acactggtgc cggatgtagg tccgattttg gcagcggtga tagctgtttg cgttgctttg
      841 ttggaaggtt ctacccggct gtatttttca gacgatgtga tcatgaataa tttgatcatt
      901 gccggtatta catttgctac ctacctggtc ttgattaatc ttaagaattt ctttgtgcga
      961 cccatcatta tgggacgtag cctgcatatg aatgaggcgc tgatcttcat cgccatccta
     1021 acggcaacga ttctatgggg aattcttgga gcattgttaa ttgtgcctgt catggcatcg
     1081 atttcggtta tcatggaata tctacgtcgc cgattgcttg gtttacctcc atttgacggc
     1141 gatggactaa aacagtttaa agctccgcct gaaaaggttc aaacacgttc gttgaaaatc
     1201 cctacaaagg ataaacaaaa gaaacaaaaa tgaaaattac gtattcaatt attgctccga
     1261 tttataacga gatagataat ctttctgaat tgtatcgtcg cgtaaaagaa gtgatggacg
     1321 ctaatggtga accatgggag tttatccttg tagacgatgg ctcaacggat ggctcgacag
     1381 ataagattcg cgaattggcg gagaaagaca agacaattcg cccagtgatc tttgcgcgta
     1441 attttggaca tcaagttgcc attactgccg gttgggacta tgcccgtggg gatgcggttg
     1501 ttattattga cgcagacctg caagacccgc ctgaagtcat cctcgagctt gccaagaaat
     1561 ggaaagaagg gtacgaggtt gtttacgcag tacgtggcga acgcgaaggt gaatcctggt
     1621 tcaagctatg gacagcatcc atgttctatc gccttatcta tcgcatcact gatgtgaaga
     1681 ttccggtgga cacaggtgac ttccgtttga tggaccgcaa agtagtgaat gttctcaagc
     1741 agatgaaaga gcgtcatcgc ttcccacgtg gcatgtctgc ctgggtgggg ttcaaacagg
     1801 ttggtgtgac gtataaacgt gctgcccgcc atgccggtat aacgaaatat ccattcagga
     1861 aaatgttacg attggcagtg aacgcaatta ccggtttttc atatttcccc ttgcaggttg
     1921 caacgtatat cggttttatc tcagccggta tttctatttt tgcaattccg gtggtggtta
     1981 tcatgcgcat ggctggaagc ggcgcctttt ttgggcaagc tactaccttg attgctgttt
     2041 tattcctggg cggtgttcaa cttatctcgc tcggtattct tggcgaatat gttggtcgtc
     2101 tttacgatga agctaagggc agaccccttt atatcgttcg tgatgctcct gaagattaac
     2161 tcccctgaat agtttgttcg ataggtcgtt ggcgtgaatg gtcagcgacc tattttcttc
     2221 ttttgaaatc cttaaatttt ttcggacgta tatgttcaaa tctctttcct tccttaaaga
     2281 ccgggctttt ctaagcgaga tgctcaccat cgcattgccg atctcttttc aacaactgat
     2341 caacgcttct ctcaacatga tcgacgtcat catggtgggg cagttgggcg aaacatccat
     2401 tgctgcactt g
//
LOCUS       DAOQJE010000606         2409 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_32407_length_2409_cov_3.187766, whole genome shotgun sequence.
ACCESSION   DAOQJE010000606 DAOQJE010000000
VERSION     DAOQJE010000606.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2409)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2409)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2409
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32407_length_2409_cov_3.187766"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..499
                     /locus_tag="PLT08_14050"
     CDS             <1..499
                     /locus_tag="PLT08_14050"
                     /inference="COORDINATES: protein motif:HMM:NF024970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNE05645.1"
                     /translation="LSSNRKERAAEIVEQSIARARSTLAESRAAIDNLRAVHFNAMDA
                     VREQVEHFKQSTGMACEEELSVTENQLDSEIIDHTMNILSESLTNISRHAQATQVKVK
                     FFIQNQTLELEVRDNGKGFDVKQETKGHYGLVGMHERARLTGGQLAIESDASGTCVQF
                     VVGRV"
     gene            642..1292
                     /locus_tag="PLT08_14055"
     CDS             642..1292
                     /locus_tag="PLT08_14055"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HNE05646.1"
                     /translation="MVRVMITDDHLIVREGLRLILETADDVEVVGEAVDGAACLELVS
                     KLNPQVILMDLQMPRMDGITAIGHLRKDFPEIAIVILTTYNEDDLMIRGLQAGARGYL
                     LKDSSRESLLDSIHAAVRGETLLKPEILARVLAPSARGKPESTAQTESTLTLTGRELE
                     VLKLAAKGERNKEMAYKLGITERTVKAHLQSIYQKFGVDSRAAAVAVAAGKGLLEN"
     gene            complement(1307..2083)
                     /locus_tag="PLT08_14060"
     CDS             complement(1307..2083)
                     /locus_tag="PLT08_14060"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HNE05647.1"
                     /translation="MKKFTIEEDYLGEFVDPVNYDLEVGGDDPSHQFYIDLAKQVGGR
                     TLEVACGTGLVAIPLLEQGIPLTGVDIVPGMLDQAKRKSEALGLSVRWIQGDARTFDL
                     NEKFNFIYMTGNAFQAFLNNSDQLAMLGQIRKHLADGGVWAFETRNPNWTDCSTDLNE
                     TEWMTYINDKGYTVRITETREYDHVEQVLVYNLFRRWEDHGESKERTTHISIRYTWPQ
                     ELNALLERTGFQVVNQYGNWDKSPLTKDSPSIISVCKLSK"
     gene            2124..>2409
                     /locus_tag="PLT08_14065"
     CDS             2124..>2409
                     /locus_tag="PLT08_14065"
                     /inference="COORDINATES: protein motif:HMM:NF013422.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor"
                     /protein_id="HNE05648.1"
                     /translation="MSNNNRTVWSSSDGDLRKKEQTTSHAKSLPPQQQTAYLHRESGG
                     RGGKVVSVVKNLVLTEEDLKSLAKKLKQECGTGGTVKDGVIEIQGEHRQRM"
BASE COUNT          593 a          581 c          661 g          574 t
ORIGIN      
        1 tctctcttca aatagaaaag aacgcgccgc cgagatcgtg gagcagtcca ttgcgcgagc
       61 gcgtagtacg ttggctgaat cccgtgctgc gattgataac ctccgcgctg tgcatttcaa
      121 tgccatggat gcggtgcgtg agcaagtgga acatttcaaa cagtccacgg gtatggcgtg
      181 tgaagaggag ctttcggtta cagagaatca acttgactcc gaaatcattg accatactat
      241 gaatatcttg agcgagtcgc tcacgaatat ttcccgtcat gcacaggcaa cacaggtgaa
      301 ggtgaagttc ttcattcaaa accaaaccct ggagttggaa gtccgcgata atggcaaagg
      361 gtttgatgtg aagcaggaaa cgaagggaca ctacggcttg gtcggtatgc acgaacgcgc
      421 gcggctgacg ggcggtcaac tggcgattga gtcagatgcg agcggaacgt gtgtgcaatt
      481 tgtggtgggg agggtgtgat gagagtcgat ggagactatt tcttttgtac acggattaca
      541 cgggtttcac ggattaacac ggaagggaaa aataaaaaaa tccgtgttcg tctgtgtgat
      601 ccgtcaaatc cgtgtactaa tgtatttgtg aggacttcta tatggttcgc gtgatgatca
      661 ccgatgacca tctcatcgtc cgcgaggggc tgcggttaat tcttgaaacg gcggatgatg
      721 tcgaagtggt cggtgaagcg gtggatggcg cggcgtgcct tgaactcgtc tcgaaattga
      781 atccccaggt tattctcatg gatctgcaaa tgccgcgcat ggacggtatc actgccatcg
      841 gtcacttgcg caaagatttt cccgaaattg ccatcgtcat cctcaccacc tacaacgaag
      901 atgatttgat gattcgcggt ctgcaagctg gcgcgcgcgg atatttattg aaggattcaa
      961 gtcgcgaaag tctattggat tcgattcatg cggctgtaag aggcgagacg ttgctcaagc
     1021 cggagatcct tgcccgcgtg ctcgcgccat ctgctcgggg caagcccgag tctacggctc
     1081 aaaccgaatc cacgctgacc ctaaccgggc gtgaactcga agtattaaaa cttgccgcga
     1141 agggagaacg caataaagag atggcgtaca agttgggaat tactgaaaga acggtgaagg
     1201 cgcatttgca aagtatttat caaaaatttg gagtcgattc gagagcagcg gctgtcgccg
     1261 tggcggcggg gaaggggttg ttggagaatt aaaagtgaca gtcaccttat ttggataatt
     1321 tgcaaaccga aataattgag ggactatctt tcgtcagcgg gcttttatcc caattaccat
     1381 attgattcac aacctgaaac cccgtccgct cgagcagggc gttcaattcc tgcggccatg
     1441 tatagcggat ggaaatgtgg gtcgtgcgtt ccttgctttc cccatggtct tcccaacgcc
     1501 gaaataaatt gtagaccaat acctgctcaa catggtcata ctcccgtgtc tctgtaatgc
     1561 ggacggtgta gcccttgtcg ttgatgtagg tcatccattc ggtttcgttc aggtcggtgg
     1621 agcaatctgt ccaattgggg ttgcgggtct cgaacgccca cacaccaccg tccgcgagat
     1681 gtttgcggat ctgtcccagc atggcgagtt gatcggaatt attcaaaaag gcttgaaagg
     1741 cgttgcccgt catatagatg aagttgaact tctcgttcag gtcgaatgtg cgggcgtctc
     1801 cttgaatcca gcggacggac aaacccaacg cctcagactt ccgcttcgcc tggtccaaca
     1861 tccccggcac gatgtccacg ccggtgaggg gaatgccctg ctctaaaagc ggaatggcaa
     1921 ccagccccgt gccacatgcc acttcaagag tcctcccgcc gacttgcttg gcaaggtcga
     1981 tgtagaactg gtgagacggg tcatcaccgc caacttccag gtcgtagttg accgggtcaa
     2041 cgaactcgcc gaggtaatct tcttcgatgg taaatttttt catgccatga gtataacgga
     2101 cttcttttgg ctataattgc cccatgtcca ataacaatcg caccgtctgg tcttcctccg
     2161 atggagatct tcgcaagaaa gaacaaacta catctcacgc taaatccctg ccgccgcaac
     2221 agcagaccgc ttatcttcat cgcgagtcgg gcgggcgcgg tgggaaagtt gtttccgtag
     2281 tgaagaatct tgtcctcact gaagaggatt tgaaatccct cgccaagaaa ctcaagcaag
     2341 aatgtggcac gggcgggacg gtcaaagatg gcgtgatcga aattcaagga gaacaccgtc
     2401 agcgcatgg
//
LOCUS       DAOQJE010000607         2401 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_32611_length_2401_cov_3.400682, whole genome shotgun sequence.
ACCESSION   DAOQJE010000607 DAOQJE010000000
VERSION     DAOQJE010000607.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2401)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2401)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2401
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32611_length_2401_cov_3.400682"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..630
                     /locus_tag="PLT08_14070"
     CDS             <1..630
                     /locus_tag="PLT08_14070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE05649.1"
                     /translation="DFAIELVNSQNIYPDGCDSLPTETVSQPQQSGMNCSFIDELMSK
                     ADVIKNLYEGQSLAGVQAKLIYPVDVPAGWTVQYDGQDYNAPVHFDSGIVASFWSPNS
                     CRPLSIGNSSTTVSQDSRDIAAQTLGYANLDNFMQAFGIPSEVKTRIFVCPNEETWCL
                     SVYEEPGQPDFHFNNVSSCTIDGKQANGTSTIPEGFDGLIKGFTIRPCR"
     gene            826..1134
                     /locus_tag="PLT08_14075"
     CDS             826..1134
                     /locus_tag="PLT08_14075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE05650.1"
                     /translation="MSKILLGIICGIVFGIISVITMLPLKLEDKKTAMAGAFANRFAV
                     GFVIGATDLPLPMWASGLLFGLLLSLPDAIITKSWIPIMTLGAIGGVLCGLAIGAFGI
                     "
     gene            1147..1497
                     /locus_tag="PLT08_14080"
     CDS             1147..1497
                     /locus_tag="PLT08_14080"
                     /inference="COORDINATES: protein motif:HMM:NF039496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5655 domain-containing protein"
                     /protein_id="HNE05651.1"
                     /translation="MTTDFIVENHFENKSPVVREIYDGLIKKVKSFGKFQEEPHKTSI
                     HLLNQTTFAGIATRKDYLLLTIKIDSPIKSSRVTKTEQVSRNRYHILIKLETPKEVDA
                     ELLEWLKGAYKLSG"
     gene            1540..2322
                     /locus_tag="PLT08_14085"
     CDS             1540..2322
                     /locus_tag="PLT08_14085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE05652.1"
                     /translation="MGFCAIFEHFSTPEQNPALEVLSTPAHPQVTQTVGRLSIVSKIM
                     KKSIVTIVFSLLFLMSCIGNSFNQLVNPYSPTLDISALQTSAFQTVYAEAMSTATSTK
                     TPTPSPTLTSTSVPMATPTVIFTLVYSDVIGANCVSGVAYNPNTEVLKLVTQLPSDII
                     TPPPGVDIDIGSWDLDSNCFFISIPRKHGGGYIFWDGENNIIYWSEGTTPGVPGFGKI
                     NNIGGGWGWLAFLTLTKDFKAQTDIHERILSLTGTPTSSTAP"
BASE COUNT          713 a          496 c          502 g          690 t
ORIGIN      
        1 gattttgcaa tcgaacttgt aaatagccaa aatatatatc ctgatggctg tgattctttg
       61 cccaccgaaa cggtttctca gccacaacaa agcggaatga attgttcatt tattgatgaa
      121 ttaatgagca aggcagatgt tattaagaat ctttatgagg ggcaatcgct tgcaggcgtc
      181 caagcaaaac tcatttatcc agttgacgta ccagcaggat ggacagttca atatgacggt
      241 caagattaca atgcaccagt ccattttgat tcaggcatcg ttgcatcgtt ttggtctcca
      301 aattcatgca gaccattaag cattggcaat agttctacta cggtgtcaca agattcacgg
      361 gatattgcag cacaaacatt aggctatgca aatcttgata attttatgca agcatttggt
      421 ataccaagtg aggtcaaaac aagaattttc gtatgtccaa acgaagaaac ttggtgttta
      481 agtgtgtatg aagaacctgg gcaaccagat ttccatttca acaatgtgtc cagttgtaca
      541 atagatggaa aacaggcaaa tggcacttct acaatccccg aaggatttga cggattgata
      601 aaagggttta ccatccgacc atgtaggtag ggatgaaacg ccgccgaaca aagcgtgcac
      661 ctgacgctgg ggattctgcg ctcgtttcaa gcagtttcct acgccttatc atttttctgg
      721 ttggacggct tcgccgtccc tgccccagcg caggtaacgc aaaccgttag gcgggcttcg
      781 tcataattgt cgtaaaatat tgttatcgaa ggagatacat tagccatgtc caaaatctta
      841 cttggtatta tctgcggcat tgtttttgga ataatctcag taattactat gcttcccctc
      901 aaacttgaag ataagaaaac agcaatggca ggtgcttttg ccaatcgctt tgcggttggt
      961 tttgttattg gtgcaaccga tttaccttta ccaatgtggg caagtgggtt gttatttggg
     1021 ttactcctca gcctgcctga tgcaatcatt acaaagtcgt ggataccaat tatgacgtta
     1081 ggagcaattg gcggagtttt gtgcggtttg gctatcggcg cttttgggat ataaggagag
     1141 gcgagcatga ccactgactt tattgttgaa aatcattttg aaaacaaatc gcccgtcgtt
     1201 cgagaaattt acgatggtct cattaaaaag gtaaagtcct ttggtaagtt tcaggaagag
     1261 ccacacaaga catcaattca tctacttaac caaactacct ttgcgggtat tgcaactcgc
     1321 aaagattatt tattgttgac catcaaaata gattctccaa ttaagagttc aagagttaca
     1381 aaaacagaac aggtttctcg aaatcgctat catattctga ttaaactcga aacaccaaaa
     1441 gaggttgacg cagagttact tgaatggctg aaaggcgctt acaaattaag tggttagcaa
     1501 accaaaacac gcccgcctaa caaagcgtgc acttgacggg tgggattctg cgccattttc
     1561 gagcattttt ctacacccga acagaatcct gctctcgaag ttttatctac gcccgcccac
     1621 ccgcaagtaa cgcaaaccgt tgggcgtttg agtattgttt ctaaaattat gaaaaagagt
     1681 atcgtaacca tcgtcttttc attattattt ttgatgagtt gtataggaaa tagcttcaat
     1741 caacttgtta acccttattc acccacattg gatatttcag cacttcagac atctgctttt
     1801 caaaccgttt atgctgaagc aatgagcact gccacttcaa ccaaaactcc aacaccttcg
     1861 ccaactttga catcaacaag tgttcctatg gcaacgccca ctgttatatt tactcttgtc
     1921 tattcagatg taataggcgc aaattgcgta tcgggcgttg catacaatcc aaatacagaa
     1981 gttttgaagt tagtaacaca attaccttca gatataatca cgccaccacc aggagttgat
     2041 attgatatag gctcatggga tttggatagc aattgttttt tcatttctat tcccagaaaa
     2101 catggcggcg gatatatttt ttgggatgga gagaacaata tcatttactg gagtgaaggt
     2161 acaacgcctg gagttcctgg atttggaaaa ataaataaca ttggaggtgg gtggggttgg
     2221 cttgcatttc taacactaac aaaagacttt aaagcacaaa cagatattca tgaaaggata
     2281 ctcagtttga ctggcacacc aacatcatcg actgcccctt agatattttt tgatggcagg
     2341 taaaaacgcc caacaaagcg tgcacctgac gctggggatt ctgcgcaaat ctcaagcagt
     2401 t
//
LOCUS       DAOQJE010000608         2400 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_32634_length_2400_cov_5.265245, whole genome shotgun sequence.
ACCESSION   DAOQJE010000608 DAOQJE010000000
VERSION     DAOQJE010000608.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2400)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2400)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2400
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32634_length_2400_cov_5.265245"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..199)
                     /locus_tag="PLT08_14090"
     CDS             complement(<1..199)
                     /locus_tag="PLT08_14090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE05653.1"
                     /translation="MKIIACIKQVPDSEAKVTAADGKVSWGDAPLVINPFDEYAVEGA
                     LQQKEAAGGTVTALCIGPESAK"
     gene            complement(233..511)
                     /locus_tag="PLT08_14095"
     CDS             complement(233..511)
                     /locus_tag="PLT08_14095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE05654.1"
                     /translation="MTRPPFGFVLAFLLFSLLFLSNSYKLWFKTEVYYRDLYNSLTNE
                     RTPHPFKNFFMKRLENKQSWLFWQKAFSLIGIAAVAGMDIVVVMAYLK"
     gene            complement(518..805)
                     /locus_tag="PLT08_14100"
     CDS             complement(518..805)
                     /locus_tag="PLT08_14100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE05655.1"
                     /translation="MEIQPLYTSIANIIEIKTGTIVMENHNGFPAKESNLYLISPNGE
                     ILWKAEKPDITTLFTKVKLNEDATLSTFTNRGQFCDINISNGKIINSTSFR"
     gene            1036..1290
                     /locus_tag="PLT08_14105"
     CDS             1036..1290
                     /locus_tag="PLT08_14105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin domain-containing protein"
                     /protein_id="HNE05656.1"
                     /translation="MNEVKHQPKVLVFSTPTCTYCNMAKKYFRDKGIKFTDVDVSRDQ
                     AAARDMVRRSGQMGVPVIDIGGKVIVGFDRPRIDTLLGIK"
     gene            1311..1583
                     /locus_tag="PLT08_14110"
     CDS             1311..1583
                     /locus_tag="PLT08_14110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HNE05657.1"
                     /translation="MAKVNIQPLGTRILVRPLAHESKTTSGLYLPETAKEKPQTGEVI
                     AVGEDESIILRPKDRVLFAKYSGTEFKFDGVDYLLLESNDVLARLN"
     gene            1982..2068
                     /locus_tag="PLT08_14115"
     tRNA            1982..2068
                     /locus_tag="PLT08_14115"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2016..2018,aa:Leu,seq:cag)
BASE COUNT          618 a          527 c          577 g          678 t
ORIGIN      
        1 ctttcgcaga ttcgggcccg atgcacaagg ctgtaaccgt gccgcccgcg gcttccttct
       61 gctggagtgc gccttcgaca gcatactcgt cgaacgggtt gatgaccagc ggcgcatctc
      121 cccacgaaac tttcccgtct gcagccgtga ctttggcttc agagtcgggc acttgcttga
      181 tacatgcgat gattttcaag ttagactcct tgtagaaatt cgttttgttc gattacttta
      241 aatatgccat gacaactacg atatccatgc ctgcaaccgc tgcgatgccg atcaggctga
      301 aggctttttg ccagaagagc caactctgct tgttctccag ccttttcatg aaaaaattct
      361 tgaacgggtg cggggttctc tcattggtaa ggctgttgta caggtcgcga taatatactt
      421 cggtcttgaa ccagagttta taactatttg agaggaataa taagctaaag agcaggaaag
      481 cgaggacaaa tccaaaaggg ggccgggtca tttgatctta tcgaaaactt gtactattga
      541 tgatcttgcc gttcgaaatg ttgatatcgc aaaactgtcc gcggttggta aaggtggaaa
      601 gggtagcatc ttcattgagt ttcaccttgg tgaacagggt ggtgatgtcg ggtttttccg
      661 ctttccagag aatctcacca ttcggagaga tcagatacag gttcgattct ttggcaggaa
      721 agccgttgtg attttccatg acgatcgtgc ctgttttgat ctcaataata ttggcaatgc
      781 tggtgtacag gggttgaatt tccatgctta cctgtccttg gatagatttc atcttaccgg
      841 gtgggaaatt ctgcctctaa aagagccgct tcatttcaac cttgtgtatt atatcccaaa
      901 ctacccatac ctaccgctcg gtaggtgaaa tttggaagac cgttatttct catatgtaat
      961 attaataagg ggcttgtaac ttatttcgcc tgttcgcatc tactgatgta gcatctaatt
     1021 tttggaggta tcgctatgaa cgaggttaaa catcaaccca aggtattggt tttcagcacc
     1081 cccacctgca cctattgcaa tatggccaag aagtattttc gagataaagg tatcaaattc
     1141 accgatgtgg atgtcagccg agatcaagct gcggcccgcg atatggttcg ccgctcgggt
     1201 cagatgggcg tgccggtcat cgatattggt gggaaggtca ttgtcggatt tgatcgtccc
     1261 cgcattgata cactgcttgg catcaaataa catacatagg agaaataatg atggcaaaag
     1321 tcaacataca gccgcttgga actcgtattc ttgtcagacc cctggcgcat gaaagcaaaa
     1381 caacttctgg tctgtacctg ccggagaccg ccaaggaaaa gccccaaacc ggggaggtga
     1441 ttgctgtggg ggaggatgaa agcattatcc taaggcccaa ggacagggtt ctatttgcaa
     1501 aatatagcgg cacagagttc aaatttgatg gtgtggatta cttgttgctt gaatccaacg
     1561 atgtgctggc ccgtctgaac tagtcaataa aataaaaaat gcccgacgca attgagaacg
     1621 cgtcgggcta gggtgtggga tggtcttcca tcattgctct tgaactccca tattgctgag
     1681 ttcttcctgg ctgtattgct caacgaacaa acttgagccg gccaatgcga gcaggaggag
     1741 gacgatcact gctgcaagta cgaacatttc gcaactcctt tcttgtatgt acaatataac
     1801 gatttttttt gcaaaaggat actcccctaa cagtactgta atggaccgac aatacattta
     1861 tgaaagggtg tctcaacttt aacaacgaag cagatgatgc ttttgttccc atgaattcgt
     1921 atctagctca ttgacattcc actcttttga gataaaatac atcccgccaa ccgtatgata
     1981 tgccgaagtg gcggaattgg cagacgcgct acgttcaggg cgtagtgagg gttccctcat
     2041 gagggttcaa gtccctcctt cggcaccaca agacccgcat ctgcgggtct ttttctttgg
     2101 caggatcaac tttcggcgga ggaggaagag gagaatatcc ttgctatgaa tctgtatccg
     2161 taatttcagc agaatgggga taggggtggt ataattgcaa tataaataag cgctttcttc
     2221 atctaatgca gacacgaagt tttccctcat cttcgtgtct ttttgtgcca ttttctcaaa
     2281 atttagtgtt tttccaactc tgaaagcctt aatttgacct cgaattgccc gtaaaggaac
     2341 ctgtatggcc gaagaagaga ttttgaacgc tggaaatatt gaatcggtgg atattgacgc
//
LOCUS       DAOQJE010000609         2396 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_32724_length_2396_cov_6.598889, whole genome shotgun sequence.
ACCESSION   DAOQJE010000609 DAOQJE010000000
VERSION     DAOQJE010000609.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2396)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2396)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2396
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32724_length_2396_cov_6.598889"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..784)
                     /gene="rfbA"
                     /locus_tag="PLT08_14120"
     CDS             complement(<1..784)
                     /gene="rfbA"
                     /locus_tag="PLT08_14120"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008879 - glucose-1-phosphate
                     thymidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HNE05658.1"
                     /translation="MKGIILAGGRGTRLHPLTLAASKQLLPVYDKPMIYYPLSMFMLA
                     GIREILIISTPEDLPSFRRLLGDGKSWGMSFHYAEQAEPRGLADAFIVGESFINGDKA
                     CLILGDNIFFGQGLAAQLQSAAQLKQGALVFAYPVNDPERYGVIEFDAQGKAISLEEK
                     PKQPKSNFAVPGLYFFDERVVAFAKALKPSPRGEIEITDLNRVYLEQGELQVISLGRG
                     VAWLDAGTHESLLQAANFVQAVEDRQGLMISSPEEIAFRKGFI"
     gene            complement(797..1867)
                     /gene="rfbB"
                     /locus_tag="PLT08_14125"
     CDS             complement(797..1867)
                     /gene="rfbB"
                     /locus_tag="PLT08_14125"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007846915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008460 - dTDP-glucose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HNE05659.1"
                     /translation="MKNVLVTGGAGFIGSNFIHYLIQKESDVNIINLDALTYAGNLAN
                     LSSVEKHPRYRFVQGNICDADTIDSLLREYEIDTVVHFAAESHVDRSILGPRQFVETN
                     VMGTFTMLEAARTYWIKDKAFPLEHVRFHHVSTDEVFGSLKPGEPAWTEETPYAPNSP
                     YAASKAASDHLVRSYGHTYGLPYTITNCSNNYGPYQFPEKLIPLIILNAIEGKPLPVY
                     GDGQQIRDWLHVEDHCEAIHLVLTKGATGETYNIGGVNQPANLTIVETICDILDEISE
                     TTHKPHKNLIQFVKDRPGHDRRYDMDTHKIHTELGWQPRHTLTEGLLDTVKWYLDHLE
                     WVNSIRKQQDYKGWLDKNYKAR"
     gene            complement(1893..>2396)
                     /gene="rfbC"
                     /locus_tag="PLT08_14130"
     CDS             complement(1893..>2396)
                     /gene="rfbC"
                     /locus_tag="PLT08_14130"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008830 - dTDP-4-dehydrorhamnose
                     3,5-epimerase activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HNE05660.1"
                     /translation="PKIYEDPRGYFMEVYREDRFSIEGISRNFVQENQSRSQKGVLRG
                     LHYQIRQAQGKLVRVLSGEIFDVAVDIRRSAPTFGQWIGAILSSENRHQLWIPEGFAH
                     GFYVTSDSAEVAYKVTDYYAPEWERSILWNDPQIGVDWQLAKDAAPILSQKDLNGKPL
                     IDAELFE"
BASE COUNT          642 a          596 c          522 g          636 t
ORIGIN      
        1 caataaagcc tttgcgaaaa gcgatctcct ctggcgaaga gatcatcaaa ccttggcggt
       61 cttccaccgc ttgcacaaaa tttgccgcct gcaacagcga ctcatgtgtg ccagcatcca
      121 gccaggcgac gccgcgccca agcgaaatga cctgcaactc accctgttca agatacacac
      181 ggttaaggtc agtgatctcg atctcaccac gcggtgaagg cttaagcgct ttggcaaaag
      241 caacgacacg ctcatcgaaa aaatataagc ccggtactgc aaaattagat ttaggctgtt
      301 ttggtttttc ttccaggcta atggctttgc cttgcgcatc aaattcgatc accccatagc
      361 gttcagggtc attgaccggg taagcaaaga ccagagcacc ctgcttaagt tgtgcagcag
      421 attgcaattg cgctgccaac ccttgcccaa agaagatatt atcgccaaga atcaggcaag
      481 ctttatctcc attgataaaa ctctccccca caataaaagc atctgccaaa ccacgcggct
      541 cggcttgttc tgcataatga aaagacattc cccaagattt gccgtcgcca agtaaccgcc
      601 ggaacgaagg caggtcttct ggtgtgctaa tgattagaat ctcacgtatc cctgccagca
      661 taaacataga aagcggatag tagatcatgg gtttatcgta tacgggtagc aattgtttgc
      721 tcgctgccaa agtgagcggg tgcaaccttg taccgcgtcc gcctgccaaa ataataccct
      781 tcatgttttt ctccttttag cgggctttgt agttcttatc gagccagcct ttgtagtcct
      841 gctgttttcg aatcgaattc acccattcca gatgatccaa ataccattta acggtatcca
      901 gcaatccttc ggtaagtgta tggcgtggct gccagcccaa ttcggtatgg atcttatggg
      961 tatccatatc ataacggcgg tcgtgcccgg ggcggtcctt tacgaactgg atcagattct
     1021 tatgaggctt atgcgttgtt tcagagatct catccagaat atcgcagatg gtctccacaa
     1081 tggtcaggtt ggcaggctga ttcacaccac caatgttata ggtctcaccc gtagctccct
     1141 tggtcaatac aaggtggatc gcttcgcaat gatcttccac atgcagccaa tcccgtatct
     1201 gttgtccatc tccatatacg ggcaaaggtt tgccttcaat cgcattcaag attattaggg
     1261 gaatcagttt ctcagggaat tgatagggtc catagttatt cgaacaattg gtgattgtat
     1321 agggcagtcc atatgtatgc ccatacgagc ggacaagatg atcactagcc gctttcgatg
     1381 cggcatacgg cgaattggga gcataagggg tttcttccgt ccatgccggt tcgcctggtt
     1441 ttagagaccc aaaaacttcg tctgtagaaa catgatgaaa acgaacatgc tctaatggga
     1501 atgctttatc tttaatccaa taagttcgcg cggcttccaa catggtgaag gtgcccatca
     1561 cattcgtctc tacgaattga cgaggtccca aaatggaacg atctacgtga gactcagcgg
     1621 caaagtgtac aaccgtatca atctcatatt ctcgcagaag gctatcgatg gtgtcggcat
     1681 cacaaatatt gccttgcaca aaacggtagc gcgggtgctt ctcgaccgaa cttaagtttg
     1741 ccaaatttcc agcataggtc aacgcatcca aattgatgat gttgacatcg gattctttct
     1801 ggatcaaata atggatgaaa ttcgagccta tgaacccggc tccgcctgta accagaacat
     1861 ttttcattct ttagtattgt ccttatttct ctttattcga acaactcagc atctatcaga
     1921 ggttttccat tcaaatcttt ttgagaaaga ataggtgcgg catctttcgc aagttgccag
     1981 tcaacaccga tctgcgggtc gttccataaa atactacgtt cccattcagg ggcgtaataa
     2041 tcagttactt tataggcaac ttcggcagaa tcactggtga cataaaaacc atgagcaaat
     2101 ccttcaggga tccacaattg gtggcggttt tctgaagaaa ggatcgcccc aatccactga
     2161 ccgaaagttg gcgcactacg gcgaatatcc acagctacat caaaaatttc gcccgaaaga
     2221 actcgaacca atttcccctg tgcctggcgg atctggtaat gtaagccgcg caaaacacct
     2281 ttttgcgaac gagattgatt ttcttgaaca aaatttctgg agattccttc aatagaaaat
     2341 cgatcctccc ggtaaacttc catgaaatat ccacgcgggt cttcatatat tttggg
//
LOCUS       DAOQJE010000610         2396 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_32726_length_2396_cov_6.399829, whole genome shotgun sequence.
ACCESSION   DAOQJE010000610 DAOQJE010000000
VERSION     DAOQJE010000610.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2396)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2396)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2396
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32726_length_2396_cov_6.399829"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..601)
                     /locus_tag="PLT08_14135"
     CDS             complement(<1..601)
                     /locus_tag="PLT08_14135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016624 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, disulfide as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HNE05661.1"
                     /translation="MKKEQLLDLYYQMVLIRRVEERGAELYQAGKIGGFMHLYIGQEA
                     VSTGLIAARESRDRVITAYRDHGVALNCGISANEVMAELLGKATGMSKGKGGSMHMAS
                     TEKNMWGGHAIVGGHLPVAAGLALGDQYAKNDNITICMFGDGATNIGYFHEAVNLAKT
                     WGLRVLWVCENNQYGMGTAVERASAVTEIRQKAEGYGMKN"
     gene            complement(829..1551)
                     /locus_tag="PLT08_14140"
     CDS             complement(829..1551)
                     /locus_tag="PLT08_14140"
                     /inference="COORDINATES: protein motif:HMM:NF019844.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0070064 - proline-rich region binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HNE05662.1"
                     /translation="MKARRDISFAIVVLILSILACNMPSAGSETPSTGEDVSQQPSPG
                     VTYVVVVEDTATIQATETLTPEPPTPQTPPKLTLTKNSNCRLGPSTLYNVVDQIASGA
                     ELSVVGRSEDSEWWQVLNANGRECWVSGENATPNQDFSTLVVGSAPPLPGVPLNFFVA
                     NQICQSAQKKFTVTLSWSSGGGETSFRIYRDGKAIQELKSTRFNFKDVNAPYDKNIVY
                     EIVGLNENGESPRATQIVPACK"
     gene            1633..>2396
                     /locus_tag="PLT08_14145"
     CDS             1633..>2396
                     /locus_tag="PLT08_14145"
                     /inference="COORDINATES: protein motif:HMM:NF025030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HNE05663.1"
                     /translation="MDWFTNPKQNEVKKLITQLADASRRNAAAQELIRLGADSAAPLI
                     DALQTQDLNLLAIYQQILARIPAASPELTKALKSAHPIIRGRVAEVFGLNKDKTAIPA
                     LIEALHGEFFTVRARAALALANIGDRLVISQLLPLLKDQEDEVRMAVCMALGIFKDPS
                     TFDELANVTLDDPIIEVRQAAVKALGDSKNPAAIPFLMEALRDSFWWFEREQSVRVLM
                     NAIEGMGKSVVEPLIEALADREGTVRKLAAKMLGNLR"
BASE COUNT          611 a          608 c          560 g          617 t
ORIGIN      
        1 cgttcttcat gccgtagcct tccgcttttt ggcggatctc ggtcacagcc gaagcacgtt
       61 caaccgcggt acccatgcca tattggttgt tttcacagac ccaaagcacg cgcaagcccc
      121 aagtcttggc gaggttcacg gcttcgtgga agtaaccaat gttggtggca ccatcaccga
      181 acatacaaat ggtgatgtta tcgttcttgg catattggtc gccgagggcg agtccagccg
      241 cgacgggtaa gtgcccgccg acgatggcgt gtccgcccca catgttcttc tctgtgctcg
      301 ccatatgcat cgagccgccc ttgcctttag acatgcccgt tgccttgccg agcaactccg
      361 ccatcacttc gttggcagag atgccacagt tcaacgccac accgtggtcg cggtaggcgg
      421 tgatgacacg gtcacgtgac tcacgcgccg cgatcagacc agtcgaaacg gcttcctgac
      481 caatgtacag gtgcataaag ccgccgatct tgccagcctg gtacaattcc gcgccacgtt
      541 cttccacacg gcgaatcagc accatctggt aatacaaatc aagcagttgt tctttcttca
      601 ttattgggaa ctccgataag gataatacgc aaaaagcgta attttgagtt gaaaaatact
      661 taaggaaaga acaccaaatt tcctctggcg ttctgtttcc ggtaaatcaa gtcggggatt
      721 atatcagaga aagaacctcc aaattcaagg ttgataggat ttgaaagtgt taaaaaacac
      781 aactctttgc cttagcaaag agttgtgttt tttatggaaa tttgtgggtt atttacaagc
      841 tggcacaatc tgtgtcgcgc gcggagattc tccgttttcg tttaatccta caatctcgta
      901 cacaatgttc ttatcgtacg gggcgttcac atctttgaaa ttgaaacgag ttgactttaa
      961 ttcctgaatt gcttttccat cccgataaat gcggaagctc gtctcgccgc cgccgctgct
     1021 ccaactcaac gtcaccgtga atttcttctg tgccgactgg caaatctgat tggctacaaa
     1081 aaagttcagc ggcacaccgg gcaatggcgg ggcgctccca accacaagcg tgctgaaatc
     1141 ctgattgggc gtcgcatttt cgccagatac ccaacattcc ctgccattgg cattcaaaac
     1201 ttgccaccac tcgctatctt cactacgccc aaccaccgaa agttctgcgc cagaagcaat
     1261 ctgatccaca acattatata aagtactcgg acccaaacgg cagtttgaat ttttcgtaag
     1321 cgtcaattta ggcggcgttt gtggggtagg aggttcagga gtaagtgttt ctgtcgcttg
     1381 aatagtagcc gtatcttcaa ccaccacaac atacgtcaca ccggggctgg gctgctggga
     1441 tacatcttcc cccgtgctgg gggtttcact gcctgcactt ggcatattac atgcgagaat
     1501 tgagagaatc aaaacaacaa ttgcaaaaga aatatctctt cttgctttca tatggatctc
     1561 ctctagttgg ctaaagtatt cttgtaatta ttatacacgc cgcccagatt caaattcatg
     1621 taaaataaac ttatggattg gttcacgaac cccaaacaaa atgaagtgaa aaagttaata
     1681 actcaattgg cagatgcaag ccgacgaaat gccgccgcac aagaattgat cagacttggc
     1741 gctgattctg ccgcaccgtt gattgacgcg cttcaaacgc aagacttgaa cctgctcgcc
     1801 atctatcagc agattctcgc ccgaatccca gccgcctctc ccgaattgac caaagcgctc
     1861 aaatctgccc atcccatcat ccgcggacga gtagcagagg tatttgggtt gaacaaagat
     1921 aaaactgcga taccagcttt aattgaagcc ttgcatggcg agtttttcac tgtccgcgcg
     1981 agggcggctt tggcacttgc caatattgga gatcgacttg ttatttctca attactgcct
     2041 ttattgaaag accaagagga tgaggttcgt atggctgtgt gtatggcact tggtattttc
     2101 aaagaccctt ccacgtttga tgaactcgcc aatgtaaccc ttgatgaccc cataatcgaa
     2161 gtgcggcaag cggcagtgaa agcgctaggt gactcaaaga atccagcagc aattcctttt
     2221 cttatggaag cattacgcga ctcgttctgg tggtttgagc gagaacagtc tgtgcgtgtg
     2281 ttgatgaatg ctatcgaagg gatgggcaag tcggtcgttg agccgctgat tgaagccttg
     2341 gcagaccgtg aagggacggt tcgtaaatta gcggcaaaaa tgctcggcaa tttacg
//
LOCUS       DAOQJE010000611         2393 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_32794_length_2393_cov_6.952524, whole genome shotgun sequence.
ACCESSION   DAOQJE010000611 DAOQJE010000000
VERSION     DAOQJE010000611.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2393)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2393)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2393
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32794_length_2393_cov_6.952524"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            84..>2393
                     /locus_tag="PLT08_14150"
     CDS             84..>2393
                     /locus_tag="PLT08_14150"
                     /inference="COORDINATES: protein motif:HMM:NF019156.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE05664.1"
                     /translation="MLKNFVKLFSGDPTKKTVDQYRPLVEQVNALEAQFTSLSDDALR
                     AKTDEFKKRIVELTGNVEGLEEKERIKAEQNALEQIMPEAFAAVREASKRTIGLRHYD
                     VQIIGGAALHSGSIAEMRTGEGKTLVATMPVYLNSLLGRGVHLITVNDYLARRDARWM
                     APIYHALGLTVGVLQMAAVTENGKKAFIVDFERDAPQEDQRHMRLVERVEAYRADVTF
                     GTNSEFGFDYLRDNMAMRLENRVQRGHYFAMVDEVDNVLIDEARTPLIISGPASGDLE
                     WYGRMAQIVKQLKPEDYEMEEKSRTINLTEIGVSRVESMLGVQLLDPDRPEDLNPEQA
                     RLMGYLQQSLRAQFLFTRNKDYLVQGGKVVIIDESTGRLMPGRRWSDGLHQAVEAKEG
                     VKVEPESVTYATVTLQNYYRMYQKLAGMTGTALTEAEEFNKIYKLEVAPISPNLEYLS
                     YGKEATLTEIKTKDEEGYAYSYFAKVGDTKPLFFRRKDYPDVIYRTVEAKLRAIVQEI
                     VRYNALGRPLLVGTTSVESSERLSNRLKAEPVRRLMQYFLVRDHYIRANNIEEDGRLI
                     ADLVPFNEPIEKITPDALRAFIKPHGLTNISLDDDTNLQTLLDILSLPSESKDRLKKI
                     FQGGVLHQVLNARRHTEESQIIAGAGAFGAVTIATNMAGRGVDIKLGGELAEEVITAV
                     NRVLGKTGLKDPFDMTHDERRVELKKLDATAYGLYEAEVKNFLQYFDDMERVKELGGL
                     HVIGSERHEARRIDNQLRGRAARQGDPGSS"
BASE COUNT          604 a          639 c          614 g          536 t
ORIGIN      
        1 ctttctctaa caatcctcca atattcccca tgctataatc ccttcctcgg aagggacttt
       61 ccctctccat tgaaagggat ttcatgctta agaacttcgt aaaactattc agtggtgacc
      121 ccacaaaaaa gaccgtagac caataccgcc cgcttgtaga gcaggtcaat gctttggagg
      181 cgcagtttac gtccctgagt gacgatgcgt tgcgtgccaa gacggatgaa tttaagaagc
      241 ggatcgttga acttactggg aatgtagaag gtctggaaga gaaagaacgc atcaaagccg
      301 aacaaaacgc gctcgaacaa atcatgcccg aagcctttgc ggcagtccgt gaagcctcga
      361 agcgcaccat tgggcttcgt cactacgacg tgcagatcat cggcggtgcg gcgttgcaca
      421 gcggctccat tgcagagatg cgaaccggcg aaggcaagac cctcgtggct accatgccgg
      481 tctatctcaa ttccctgctc ggacgaggcg ttcacctcat tacagtcaat gattatctgg
      541 cacgacgcga tgcccgttgg atggcgccta tctatcatgc cctcggcttg accgtgggtg
      601 tgctgcaaat ggcggctgtc accgagaatg gcaagaaagc attcattgtg gattttgaac
      661 gggacgcgcc ccaagaagac cagcgtcaca tgcgcctcgt ggagcgggtc gaagcctatc
      721 gcgccgacgt gaccttcggt accaactctg agtttggttt cgattatctg cgcgacaaca
      781 tggcaatgcg acttgaaaac cgcgtccaac gtggacatta cttcgccatg gtcgatgaag
      841 tcgataacgt cctcatcgac gaagcgcgta ccccgctcat catttccggt cccgcctcag
      901 gtgacttgga atggtacggg cgcatggcgc agatcgttaa acagctcaag cccgaagact
      961 atgagatgga agaaaaatct cgcaccatca acttgaccga gatcggtgtg agccgggttg
     1021 agtctatgtt gggtgtacaa ctgctcgacc ccgatcgccc tgaagacctc aaccccgaac
     1081 aagcgcgtct catggggtat ttgcaacaat ccctgcgtgc tcagttcctt ttcactcgca
     1141 acaaagatta tttggtgcag ggcggtaaag tcgtcatcat cgacgagtca acggggcgtc
     1201 tcatgcctgg gcgtcgttgg agcgatggtt tgcatcaagc tgtggaagcc aaagaaggcg
     1261 taaaggtcga gcccgagtcg gtcacctatg ccacggtcac tttgcagaac tactatcgta
     1321 tgtatcaaaa gttggcaggt atgaccggta ccgcgctgac cgaagctgaa gagtttaata
     1381 aaatctacaa gctcgaagtc gcgccgatct cgccgaacct tgaatatctt tcctacggca
     1441 aagaagccac actcactgag atcaaaacaa aagatgaaga agggtatgca tattcctact
     1501 ttgccaaagt gggcgatacc aaaccgcttt tcttccgccg caaagattat cccgatgtca
     1561 tctatcgcac ggtcgaagcc aagttgcgtg ccattgtgca agagatcgtt cgctacaacg
     1621 cattaggacg cccactgttg gtgggtacca cttccgttga atcttctgaa cgtctctcga
     1681 atcggctcaa agcagaaccg gttcgacgtt tgatgcaata cttcctcgtg cgtgaccatt
     1741 acatccgcgc caataacatt gaggaagacg ggcgcctcat tgcggacctc gtgcctttta
     1801 acgaacccat tgagaaaatc acccccgacg ccttacgcgc cttcatcaaa ccccacggtc
     1861 tcaccaacat cagcctcgac gatgacacca acctgcaaac cttgctcgat atcttgagtt
     1921 tgccaagcga atccaaagat cgtttgaaga aaatcttcca aggcggagtg ttacatcagg
     1981 tcttgaatgc ccgccgccat actgaagagt cgcagatcat tgcgggcgcg ggtgcgttcg
     2041 gcgctgtgac cattgctacc aacatggcag gtcgtggtgt agacatcaaa ctcggcggcg
     2101 aattggcaga agaagtgatc acggcagtta atcgtgtgtt gggcaaaacc gggttgaaag
     2161 acccatttga tatgacccat gatgaacgtc gcgtggaatt aaagaaactt gatgccaccg
     2221 cttatggttt gtatgaagca gaggtcaaga acttcctgca atactttgac gacatggaac
     2281 gcgtgaaaga attgggcggt ttgcacgtca tcggttcaga acgccatgaa gcccgccgca
     2341 tcgataatca gttgcgcggt cgtgctgccc gtcagggtga ccccggatct tca
//
LOCUS       DAOQJE010000612         2393 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_32800_length_2393_cov_3.524380, whole genome shotgun sequence.
ACCESSION   DAOQJE010000612 DAOQJE010000000
VERSION     DAOQJE010000612.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2393)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2393)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2393
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32800_length_2393_cov_3.524380"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..185)
                     /locus_tag="PLT08_14155"
     CDS             complement(<1..185)
                     /locus_tag="PLT08_14155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE05665.1"
                     /translation="MDPILGVTIGLSMLFGFALGYRVGKQVKSKEILEAISRPTQYAP
                     DVANAAPEEEALLILNE"
     gene            complement(466..678)
                     /locus_tag="PLT08_14160"
     CDS             complement(466..678)
                     /locus_tag="PLT08_14160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE05666.1"
                     /translation="MRTFVIEGKPLEEVTIKVKGQSLTLTEGVEVTVIVVVASQQSFA
                     PDVATELRLNDCEVDPEKLPGKYADE"
     gene            complement(1057..1422)
                     /locus_tag="PLT08_14165"
     CDS             complement(1057..1422)
                     /locus_tag="PLT08_14165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE05667.1"
                     /translation="MQQVDYLHGMIRSEAIAYIQSLPGLTRDEFFDLMRSAEKKPVSR
                     HHSADTKETIELRIQYLVNFIKKCGDSGATISQIFDNLKYNGSNMRYAIAEAIERGLI
                     YVVRDGKKKVYKPAIGRKQ"
     gene            complement(1422..1706)
                     /locus_tag="PLT08_14170"
     CDS             complement(1422..1706)
                     /locus_tag="PLT08_14170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE05668.1"
                     /translation="MTTLILPTNPTRAYISFPHCRHHEVIVHDSYITPDGVMIARVQY
                     VGSLLEHTFEIEASRIFTEVIKDGYHAEVTTWDEQGNPIVNAASMYTVGE"
     gene            complement(1703..>2393)
                     /locus_tag="PLT08_14175"
     CDS             complement(1703..>2393)
                     /locus_tag="PLT08_14175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE05669.1"
                     /translation="KYRAINVSPIYEGEQVFENVQTGLHGYKPAGCNVMALPSDQISR
                     LDTGYRDVTSGKPAAKIVGYLGYFKTMDGLEKSVWMTISEIHEHAQKWAPENYNSEYG
                     AWKDPKKRPTMEMKTVFLALTKFMDLSGERNSKLHEAIESENEQDDDPITVEPVAQET
                     PSETTTEPIQYAVKDTDLVSPLSQWAIRTAADAWNIDISAANRQLASANLPATMSRGQ
                     FKNFVTEGVKP"
BASE COUNT          523 a          580 c          587 g          703 t
ORIGIN      
        1 cattcattca agataagcaa ggcttcttct tctggcgcgg cattagccac gtcaggcgca
       61 tactgtgttg ggcggcttat agcctcaagg atttcttttg acttaacttg cttgccaaca
      121 cgatacccaa gtgcaaagcc aaacaacata gataaaccta ttgtgacacc taaaattgga
      181 tccatttttt cctttcgggc gaagccgccc aacggtttgc tttactggct tgcgcccgta
      241 gagccttcgc ttacctgtgc gccagtgggc gcaagtccag tgcaagcggt gttagttgtg
      301 ttcttgccgc tacggtggcg tatttaccat ttgcctttgc caaccaagcc gagtttacgg
      361 gggctggact gcggcgagcc attcgcttat gaccgtaact gtctgcaact ttcgcaggtt
      421 tattcgagcg acaagcaact aacggttgtt tttacctgcg cggcgttatt catcagcata
      481 cttgccgggc agtttttcag gatcaacttc gcaatcattc aagcgcaact ctgtagccac
      541 gtcaggtgca aaactttgtt ggctggcgac cacaacaata acagtaacct ccacgccttc
      601 ggtgagagtg agcgattgcc ccttcacctt gatagtaact tcttcaagcg gtttaccttc
      661 gattacaaaa gttctcattt caaaaacctt tctgctggca cagcgtccag ccaactattg
      721 tttgacctgc atgtacatgg attttctaat caaaacttcc atgtctcata ccgttttctt
      781 tttggtttta tcacaacatc aggacaatat tctttcattg ccttctcaat aagcagcttt
      841 attaggtcgg tgatagacat cttatgtacg ctgtcttggt tgagacggtc taacaaaata
      901 tctaggtaca ccctgtagct ggttaggttg ggtcttgtct ctgggtatct cactatttgt
      961 catagactcc tgttattcgt tgcggtaaaa tgcaattgca aataggctca ctgtttgcgt
     1021 ttgctggctt tccttcgtgg ggaaagccag ctttttttat tgctttctac caatggcggg
     1081 cttgtacact ttctttttcc cgtcacgcac gacatagatc aagcctcttt caatggcttc
     1141 ggcaatagcg tagcgcatat tcgacccgtt gtatttcagg ttgtcgaata tctgcgaaat
     1201 ggtcgcgccg gaatcgccgc atttttttat aaaattgaca agatattgga tacgtaactc
     1261 aattgtctct tttgtatcgg cgctgtgatg acgcgagaca ggtttctttt ctgcggaccg
     1321 catgaggtcg aaaaactcgt ctcgcgtaag accaggcagg ctttggatgt atgcaatcgc
     1381 ctcggagcga atcatgccgt gaaggtaatc gacttgttgc atcactcccc cactgtgtac
     1441 atggacgcgg cgttgacgat aggattgcct tgctcgtccc atgtggtgac ttcggcgtga
     1501 tagccgtctt tgataacttc ggtaaaaatg cggctggctt cgatctcgaa tgtgtgctcg
     1561 aggagactgc ctacatattg gacgcgggca atcatgacgc catcgggggt gatatacgaa
     1621 tcatgcacaa tcacttcgtg atggcggcaa tgtgggaaag agatataagc gcgggttgga
     1681 ttggttggga ggatgagggt ggtcatggct ttactccttc ggtgacaaaa tttttgaatt
     1741 gcccgcgtga catagtagcc ggaagattcg cggatgcaag ctggcgatta gcggcggaaa
     1801 tgtcgatatt ccaggcatcg gcggcggttc tgatcgccca ctggctcaac ggagatacaa
     1861 ggtcggtgtc ttttacggcg tactggatgg gctcagtagt ggtttcactt ggcgtttcct
     1921 gtgcaactgg ctcaaccgtg attgggtcat cgtcttgctc gttctcagac tcaattgcct
     1981 cgtgcaattt ggaattacgc tcgccagaca gatccataaa cttggtgagt gccagaaaga
     2041 cggttttcat ctccatcgta gggcgtttct ttgggtcttt ccatgcgcca tactcgctgt
     2101 tgtaattttc aggtgcccac ttttgcgcgt gttcgtgaat ttcgctgatg gtcatccaaa
     2161 cggacttctc gagcccgtcc atagtcttga aatagccaag atacccaacg atcttcgcag
     2221 cgggctttcc gctagtaacg tctcgatacc ctgtatccaa acggctgatc tgatcacttg
     2281 ggagcgccat gacgttgcac cctgcaggtt tgtagccatg caagcctgtt tgcacgttct
     2341 cgaaaacctg ctccccttcg tagatcggag agacgttgat agcgcgatat tta
//
LOCUS       DAOQJE010000613         2386 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_32975_length_2386_cov_4.171600, whole genome shotgun sequence.
ACCESSION   DAOQJE010000613 DAOQJE010000000
VERSION     DAOQJE010000613.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2386)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2386)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2386
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32975_length_2386_cov_4.171600"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(145..660)
                     /locus_tag="PLT08_14180"
     CDS             complement(145..660)
                     /locus_tag="PLT08_14180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE05670.1"
                     /translation="MAYSDPVPDNYIVLIKKAAEGKKDIEALAAMGERAVELLGGEIT
                     VLDKNCTTELFRFGQGGPHGTPAGMPHIHWCSGQSPYTGHHEVKMRHSGKLHTELDTL
                     DNMGQFLDRNGTPAHASELPVGYFIVGEGELQKAVARDVSSLGPHYYIGKLLKALQNG
                     WYKKASGAVRF"
     gene            complement(846..1601)
                     /locus_tag="PLT08_14185"
     CDS             complement(846..1601)
                     /locus_tag="PLT08_14185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE05671.1"
                     /translation="MSVLADAIKALDPAPDKKKELTLALNLLSELSENKVKEFANEVQ
                     GSYRTAGTDENRTAPITTVVATHSEYRAYIKDDAGKIPGEVSTAIKKFVSGGSEQIIT
                     GIADLVTTAITAILGSGQATQQEVRSYYITVQARAMVRYDILAWRRLVEADGITSKIE
                     ACMAIYASKASIDVAALDLNTFLLAYEDQLTKMNFTEQQTIEYIDYAEEVYKRLRGDA
                     ISQSVTTPKSIVTSVSPISSRPGELVFFPDLTK"
     gene            complement(1714..2274)
                     /locus_tag="PLT08_14190"
     CDS             complement(1714..2274)
                     /locus_tag="PLT08_14190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE05672.1"
                     /translation="MPNYKVYVRGHGGEEAHMLPPSENLPIDMITIGQFGSTMSDKVA
                     DQYIFRHFGIEDIIDQINDEIVIYWTKTQRDEWYEHKRLNYSEPTLDITPYITLSQNL
                     ALDGDSSIGECGVCYWNEPAGKLEWIIKLKDRETILLSDILCVLKNMLNEGDTIQLFW
                     TACMSSRYWIGTKKKVSFNPEKTTDD"
BASE COUNT          585 a          581 c          514 g          706 t
ORIGIN      
        1 tatgccattt gcactcacct gtggtaactc agtcaatcgg ttttgatgcc taacgtcaaa
       61 gttcaccggc ggccatgagt agcatggacg tccgtgtgca acgaccagtt aggcaagtgt
      121 tgcatttgat cgttgaactt gcactcagaa gcgaacagca ccgctggctt ttttgtacca
      181 cccattttga agcgctttta ggagcttccc aatgtaataa tgtgggccaa gagacgaaac
      241 atctcgtgcc acagccttct gtaactcacc ttccccgaca ataaaataac cgacaggcaa
      301 ttcgcttgcg tgcgctgggg ttccgtttct atcaaggaac tgtcccatat tatccaatgt
      361 gtcaagctcc gtatggagct tcccggagtg gcgcatcttt acctcgtgat gtcctgtata
      421 tggagactgg ccggaacacc aatgtatgtg cggcattcca gccggcgtgc catgaggccc
      481 gccttgacca aacctaaaaa gctccgtcgt gcagtttttg tcaaggaccg taatttcacc
      541 accaagcaat tcgacagccc tttctcccat tgcagccaat gcttcgatgt ctttctttcc
      601 ctcggccgcc tttttgatta gaacaatgta gttgtcaggg actggatcgc tatatgccat
      661 ttgcactcac ctgtggtaac tcagtcaatc ggttttgatg cctaacgcct cacatcaccg
      721 gcatcgaaaa gcaagcgtag cgctgctttt tgatgtccgg tgtatgtgat tgttaggcgc
      781 ggcttatgga gtagaaatgc cgggtaatca gaacgcaaat tcaaaaccta cttagattgt
      841 gccccttatt tagtcagatc ggggaaaaac accaactcgc cgggtctgct tgaaataggg
      901 ctgaccgatg tcacaatcga tttaggggta gtaacagact gcgaaatagc gtcgcctctc
      961 agcctcttat acacttcctc agcataatcg atatattcta ttgtttgttg ctctgtgaag
     1021 ttcattttcg ttagttgatc ttcgtatgca agcaaaaaag tgttgagatc taaagccgct
     1081 acgtctatag aggctttaga tgcataaata gccatgcaag cctcaatttt agatgtaatg
     1141 ccatctgcct ctacaagtct tctccacgcc agtatgtcat atcgaaccat agcgcgcgct
     1201 tggacagtga tgtagtagct gcgcacctct tgctgcgtag cttgacctga accaagaatg
     1261 gcagttatgg cagttgtaac cagatcggca attcctgtaa tgatctgctc ggaaccacca
     1321 gacacgaatt tcttaattgc ggttgaaacc tctccgggta ttttcccagc gtcatctttg
     1381 atataagcac ggtattcaga atgagttgcc actactgtag tgatgggtgc agttctattt
     1441 tcgtctgtcc ccgcagtgcg atagcttccc tgaacttcgt tggcaaattc ttttacctta
     1501 ttttccgaaa gttcacttag cagattcagg gctaacgtga gttctttttt cttgtctggc
     1561 gctggatcaa gcgcttttat tgcatcggct agtacggaca tgacgtttct ccttagattg
     1621 gttagaaaga caaaatataa aaatggttgt agccgtggtt atttaactga gatcttttac
     1681 ataatcatgg gagcacctcc ttcttaataa atgttagtcg tctgttgtct tttctggatt
     1741 aaacgatacc ttttttttag ttccgatcca gtacctggaa ctcatgcaag ccgtccaaaa
     1801 tagctggatc gtatctccct cattgagcat attctttaac acgcaaagta tgtccgaaag
     1861 caggatcgtc tcgcgatctt ttaatttaat gatccattcc aactttcccg caggttcgtt
     1921 ccaataacaa accccacatt ccccaataga actgtctcca tctagagcca gattctggct
     1981 cagcgttatg tatggggtga tgtccaaagt tggctctgaa taattcagac gtttatgctc
     2041 ataccactca tcgcgttgcg tcttggtcca ataaatgaca atctcatcat taatttgatc
     2101 aatgatgtcc tcgatgccaa agtgacgaaa tatatattgg tcagccactt tatctgacat
     2161 cgtgctccca aactgaccta tcgtgatcat atcgattggg agattctccg acggtggtag
     2221 catgtgcgct tcttcacccc catgacctcg aacataaact ttataatttg gcactttgat
     2281 cctctcttca ttgaatgtgg tttatataca ctgcggtcgt tgccgcctaa cgtgcaagct
     2341 cagccgacgg caaaaagcgc agctttttga cggtcggctg gagcgg
//
LOCUS       DAOQJE010000614         2385 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_33006_length_2385_cov_3.188412, whole genome shotgun sequence.
ACCESSION   DAOQJE010000614 DAOQJE010000000
VERSION     DAOQJE010000614.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2385)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2385)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2385
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33006_length_2385_cov_3.188412"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..431
                     /locus_tag="PLT08_14195"
     CDS             <1..431
                     /locus_tag="PLT08_14195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE05673.1"
                     /translation="LDYVNYTKKDALRVLQEELGWRYYGGKHYESIYTRFYQGYILPE
                     KFGYDKRRCHVSSLICSGEMTREQALEEIKSPTYAPSMQEEDREYVVKKLGLTDDEFT
                     AIINAPKKSYWDYPSYGQFMRQPFMLKLVPFAKKVVSWFK"
     gene            431..1519
                     /locus_tag="PLT08_14200"
     CDS             431..1519
                     /locus_tag="PLT08_14200"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HNE05674.1"
                     /translation="MRIAYVTIHIAPEIMRGGVGKKIRTHIKLWREHGHEVTLFSLTP
                     AEIPFPEERQFLFDAKTNLLKRESNRASGMTRMLKAVREYQPDIIYLRFGLYSYPLHE
                     IHKIAPVVVDTNSNDVDEYRKKSVFFYWLNRLTRNLTFGPASGVVSPSHELVDILVPR
                     HDKPVCVVANGVDLTDVEILPAPKNTYPVLTLVGTPGMSWHGVDKLIEFAKRYPEITI
                     NIVGYGPGDVNGDVPANVKLHGFLNSQQVKDVLAGTDVACGTLAFHRNKMHEASALKV
                     REALTFGIPVLIAFRDTDLHDVHVDTILQIPNTEDNLVTHAEQVHDFAYRMMGKRVDL
                     SVVGPYLDQRKKEQKRLAFFEQVLVGKK"
     gene            1519..>2385
                     /locus_tag="PLT08_14205"
     CDS             1519..>2385
                     /locus_tag="PLT08_14205"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNE05675.1"
                     /translation="MSYSIVLLGTQMAVGGAQKLLLEQALWFQQHGHKVTVLFFYDRD
                     NLHEKWTKTYPFEIYNLEAFDKKAGGFRSLPKLMGGLLKLWQILKRERYDAIITFTHD
                     SNVLGLPLAKLAGIRSRVGTHLGEIRGMSKWRDGLHTFLINRGVIQTLVASSARTRNN
                     AIEVGVQPERITTIYNAIMPFDVAHIDRESVRAKIGLKKDEVFFLAVGRLVYEKGHEF
                     LIEAMAEVAKVDTHAIAGICGAGPLKDQLQTQIEKLNVQNNMKLLGQWGSIPEILAAA
                     DVFVLPSRWEGLP"
BASE COUNT          619 a          519 c          592 g          655 t
ORIGIN      
        1 ttctggatta tgtcaactac accaaaaagg acgccttgcg tgttttgcag gaagaattgg
       61 gctggcgtta ttacggtggc aaacattatg agtctatata cacccgtttt tatcaaggct
      121 atatcctgcc cgaaaaattt ggctatgata aacgtcgttg tcatgtttcc agcctgatct
      181 gttctggtga gatgactcgt gaacaggctt tggaagagat caaatcaccg acctatgctc
      241 cgtccatgca ggaggaagat cgtgagtatg tcgtcaaaaa acttggctta actgatgatg
      301 aatttacggc aattatcaat gctccgaaaa agagctattg ggattatccc tcttatgggc
      361 aattcatgcg ccagccgttc atgctaaaac ttgttccatt tgcaaagaaa gttgtgagtt
      421 ggtttaaata atgcgcattg cctacgtcac cattcatatt gcgccggaga tcatgcgtgg
      481 cggagttgga aaaaaaattc gcacacatat caagttatgg cgtgagcatg gacatgaagt
      541 taccctgttc agcctgaccc cggcggaaat tccatttcct gaagaacgtc agtttctttt
      601 cgatgcaaag accaatcttc tgaaacgcga atcaaatcgc gcgtcaggta tgacgcggat
      661 gttgaaggct gtccgcgaat atcagcctga tattatctac ttgcgctttg ggttgtactc
      721 ctatccgctg catgaaattc ataagatcgc gccagtagtg gtggatacga attccaatga
      781 tgttgatgaa tatcgtaaaa aaagtgtttt cttctattgg ctaaaccgcc tcacccgtaa
      841 cctgacgttt ggtcctgctt cgggagttgt ttctccctct catgagttag tcgatattct
      901 cgtgccccga catgacaaac ctgtttgcgt ggtcgccaac ggcgtggact taaccgatgt
      961 ggaaattctg cctgctccca aaaacacgta tccggtgttg acgttggttg gcactcccgg
     1021 tatgagttgg cacggcgtgg acaagttaat tgaatttgcc aaacgatacc cagagatcac
     1081 tattaacatc gttggctatg gaccgggtga tgtgaatggg gatgttcctg caaatgtaaa
     1141 actgcatggt tttctaaact ctcaacaagt taaggatgtt ttggcaggta ccgatgtagc
     1201 ttgtggaacc ctggcttttc accgtaataa aatgcacgaa gcttcggcat tgaaggttcg
     1261 tgaggcgctc acctttggaa tccctgtgtt gatcgctttc cgcgataccg acctgcatga
     1321 tgtgcatgtg gatactattc tgcaaattcc aaatactgaa gataaccttg ttacccatgc
     1381 cgaacaggta cacgattttg cctatcgtat gatgggcaaa cgcgtggact tgagtgtggt
     1441 tggtccatac cttgaccaac gcaaaaaaga acaaaaacgg cttgccttct ttgagcaggt
     1501 tctggtggga aagaaataat gtcgtattcc attgttttgc ttggcactca aatggcggtg
     1561 ggaggggcgc aaaagctatt actggaacag gcgttatggt tccagcaaca cggacataaa
     1621 gtgaccgtcc tctttttcta tgaccgtgac aacttgcacg aaaaatggac gaagacctat
     1681 ccctttgaaa tttacaacct ggaagccttc gataaaaaag cgggtggatt tcgcagcctg
     1741 ccaaagttga tgggcggctt attgaagttg tggcagattc ttaaacgcga gagatatgac
     1801 gccatcatca cctttacaca tgatagcaac gtactgggac ttccgcttgc caagctggca
     1861 gggattcgct ctcgcgtagg cacgcacctc ggtgagattc gcggtatgtc taaatggcgc
     1921 gatggattgc acacctttct gattaatcgt ggcgttattc aaaccctggt ggcatcctcg
     1981 gcgcggactc gaaacaatgc catcgaagtg ggtgttcagc cagaacggat caccactatt
     2041 tacaacgcta ttatgccttt cgatgttgca cacattgacc gtgagtctgt gcgtgcaaag
     2101 atcggcttga aaaaggatga ggttttcttt ttggcggtag ggcggcttgt ctatgaaaaa
     2161 ggacatgaat tcctgattga agccatggct gaagttgcaa aagtggacac tcatgcaatt
     2221 gctggaattt gtggggcggg tccattaaaa gaccagttgc aaacacagat tgaaaaattg
     2281 aatgtccaaa acaatatgaa attgcttgga caatggggct ccattcctga gattctggca
     2341 gcagcggatg tgttcgtgct gccttctcgc tgggaaggtt tgccc
//
LOCUS       DAOQJE010000615         2381 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_33114_length_2381_cov_5.201204, whole genome shotgun sequence.
ACCESSION   DAOQJE010000615 DAOQJE010000000
VERSION     DAOQJE010000615.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2381)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2381)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2381
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33114_length_2381_cov_5.201204"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..455
                     /locus_tag="PLT08_14210"
     CDS             <1..455
                     /locus_tag="PLT08_14210"
                     /inference="COORDINATES: protein motif:HMM:NF019325.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="CARDB domain-containing protein"
                     /protein_id="HNE05676.1"
                     /translation="SNAYKTTQNDLAVANLQFSTTASGTNVPTTTSVATTLTKGTAQL
                     SLTVTPSATTYNTAGQTITFTYTIKNTGSGDLGPTQFFINDPLMNPATLNCGAANTTL
                     APNATVQCTNNYTITDADLGAVNIQFSSSAGGTGAPTSQSVPTTLTKQ"
     gene            568..2376
                     /gene="pepF"
                     /locus_tag="PLT08_14215"
     CDS             568..2376
                     /gene="pepF"
                     /locus_tag="PLT08_14215"
                     /inference="COORDINATES: protein motif:HMM:TIGR00181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligoendopeptidase F"
                     /protein_id="HNE05677.1"
                     /translation="MTTPLPRSKVKKNQTWNAESVFKTPEAFDTELQNILADLPKIKK
                     YQGHLGENIEMFLEAFQAIDKLEKRTAKVRVYATMASAVDANDEQGAAMSSKAMSMLA
                     QVGAATSFVEPELLSMGEAKLRQWVNEDSRMKLYEHFLNDLFRKQAHVRNAEVEELLG
                     MVRDPFGAVRNTAGMLANADFKFKPAKDSKGRKLELTQSTRSALIESADRKVRQTSWE
                     NYNDKYLEFKNTLAGNLAASIKQNVFNMKARNFNSSLEATLFNGDVPVEMFHNLLDIF
                     KKKLPMWHKYWRIRRKALGVKVLHPYDVWAPLTTKKHKVPFETAVDWICEGLAPMGDE
                     YVNTMRKGCLEDRWVDWSPNAGKREGAFSTRVPSDTHPFIMMSYTDDVGSMSTLSHEL
                     GHSIHAWYASRAQPMMYYLYPSIIAETASNFNQAMTRAHLLKTKKDKYFQIALLEEAM
                     DNFHRYFFIMPTLARFELETHQRAEKGQSLTADYMNNLMADLFSEGFGGEMHLDRERV
                     GITWGTFTTHLYIDYYSFQYAIGISAANAIAKRVLDGVPGAAQDHINFLKAGSSMSPM
                     EVYKTAGIDMTSTQPIEDAFAVLEEYIDRLGELTGK"
BASE COUNT          703 a          624 c          528 g          526 t
ORIGIN      
        1 gttccaacgc ctataaaacc acccaaaacg acctggcagt ggcaaacctt caattcagta
       61 caacagcatc tggaacgaac gtaccaacta caacttcagt tgccaccacc ctcacaaaag
      121 gaactgctca actcagcctt acagtaacac cgagtgccac cacatacaac accgctgggc
      181 agaccatcac cttcacctac acgatcaaga acactggcag tggagatcta ggtcctacgc
      241 aattcttcat taatgaccca ttgatgaatc cagccacatt gaattgcggt gcagccaata
      301 ccacactggc acccaatgca accgtgcagt gtacaaacaa ttacaccatc accgatgcag
      361 acttgggcgc tgtaaacatc cagttcagca gcagcgccgg tgggacgggt gcccccacat
      421 cgcaaagtgt tccaactacg ctaaccaagc agtaagtctg tcgtttcagg aagatgacaa
      481 aatgtactcc gatattttgg caacatagct aaaaatacgg agtacatttt tatttatctt
      541 tcagcgcaca ttgaaaggaa ctgtatcatg actacaccac taccccgctc aaaagtaaag
      601 aaaaaccaaa cttggaatgc agaaagtgtc tttaaaacgc ctgaagcctt cgatactgaa
      661 ttgcaaaata ttctagctga ccttccaaaa atcaaaaaat accaagggca tcttggtgaa
      721 aacatcgaaa tgtttcttga agcgtttcaa gcgatagata agctcgaaaa gcgcactgca
      781 aaggtgcgcg tgtatgccac catggcgagc gccgtggacg caaacgacga gcaaggcgct
      841 gcgatgagca gcaaagccat gtccatgctg gcacaagttg gggcagcgac atcatttgtg
      901 gagccggaat tgctgtccat gggtgaggcg aaattacgtc aatgggtgaa cgaagactcg
      961 cgcatgaaac tgtacgagca cttcctcaat gacttattcc gcaagcaagc gcatgtgcgt
     1021 aatgccgaag tggaagaatt gcttggcatg gtacgcgacc cgttcggggc agtccgcaat
     1081 acggctggaa tgttggcaaa tgccgatttc aaattcaagc ccgccaaaga cagcaaaggc
     1141 agaaagctcg aattgactca aagcacaaga agtgctctca ttgagtcagc agaccgtaaa
     1201 gtgcgccaaa cttcttggga aaattacaac gataaatatc ttgaattcaa gaatactctg
     1261 gcagggaatc tggctgcttc gatcaaacaa aacgtattca acatgaaagc acggaatttc
     1321 aactcttcac tggaagcaac acttttcaat ggcgatgttc cggtcgaaat gttccataac
     1381 ctattggata tttttaagaa aaaactaccc atgtggcaca agtattggcg cattcgccgc
     1441 aaggcattag gtgtaaaagt ccttcaccct tatgatgtct gggcgccgtt gactacaaag
     1501 aaacacaaag ttccattcga aaccgctgtg gactggatct gtgaaggtct cgcgcccatg
     1561 ggcgatgaat atgtcaacac catgcgcaaa ggttgtctcg aagaccgctg ggtagattgg
     1621 tctcccaatg cgggcaaacg tgaaggggca ttctctactc gcgtccctag tgatacacat
     1681 ccgttcatca tgatgagcta tacagatgat gtcggctcga tgagcacgct ctcgcatgaa
     1741 ttggggcact ccattcatgc ttggtatgcc agccgtgcgc agccgatgat gtattatctc
     1801 tatccctcca tcattgcaga gaccgcctcg aactttaatc aggcaatgac ccgcgcccac
     1861 ctgctcaaga cgaaaaaaga taaatacttc cagatcgctc tactcgaaga agccatggat
     1921 aacttccacc gctacttttt catcatgccc actcttgctc gtttcgaatt ggaaacgcat
     1981 caacgcgccg aaaagggaca gtctctcaca gccgattata tgaacaacct catggcagac
     2041 ctattcagcg aaggctttgg cggagagatg cacctcgacc gggaacgggt cggtatcact
     2101 tggggaacat tcaccacgca tctttatatt gattattact ccttccaata cgctatcgga
     2161 atctccgctg caaacgccat tgccaaacgc gtgctagacg gagtccccgg cgcggcgcaa
     2221 gaccatatca actttctcaa ggcgggttcc tccatgtccc ctatggaggt ttacaaaacc
     2281 gccggcattg acatgacttc cacccaaccc atcgaagatg catttgccgt gctggaagaa
     2341 tacattgata gactcggcga attaacgggt aaataaccag c
//
LOCUS       DAOQJE010000616         2380 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_33142_length_2380_cov_5.507097, whole genome shotgun sequence.
ACCESSION   DAOQJE010000616 DAOQJE010000000
VERSION     DAOQJE010000616.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2380)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2380)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2380
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33142_length_2380_cov_5.507097"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..358
                     /locus_tag="PLT08_14220"
     CDS             <1..358
                     /locus_tag="PLT08_14220"
                     /inference="COORDINATES: protein motif:HMM:TIGR01727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNE05678.1"
                     /translation="LAVIFVTHDLGVVAELCDKVLVMYGGVTAEYADVDVIYNSPQHP
                     YTQELLKAFPDLTKPKKKLSSIPGYPPRLDNLPQGCRFAPRCPAAFDRCHTEEPLLHT
                     IANGHVVSCHLIEKSK"
     gene            355..1353
                     /locus_tag="PLT08_14225"
     CDS             355..1353
                     /locus_tag="PLT08_14225"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNE05679.1"
                     /translation="MKNTTEKLLEIRGLKKYFEAGRPSLFSRETKHVHAVDGVNLTLR
                     RSEVIALVGESGCGKSTLGLLLMGLEDPTDGSVIFEDRDITHLNDHQRKDLRRKIQMV
                     FQDPYESLNPTQTIEEIVTEPLTVHGMASSHQDRDERVRKAMEDAGLKPADVYLHRYP
                     HELSGGQRQRVVIASALVLEPEIIIADEPVSMLDVSIRAEVINLLAELRISRQIAVIF
                     ITHDLGSVGFFADRVAVMYLGRIVEIGTMLEVLEKPQHPYTKALLSVIPVPNPRLRHE
                     RIVLQGETPNPINIPSGCRFHPRCPAAFDACKLSDPPMTTLSKSHQAACLLVTNTK"
     gene            1354..2127
                     /locus_tag="PLT08_14230"
     CDS             1354..2127
                     /locus_tag="PLT08_14230"
                     /inference="COORDINATES: protein motif:HMM:NF012797.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HNE05680.1"
                     /translation="MDITSLQNPRVKHIVKLREDKKQRQKDRLILVEGYDELTLALSC
                     GFEPQTLLSAPELVSQSLTVPNAENLTVSRAVFEKISYRDNPDGWLGLFPMPTSSLDE
                     LKLSENPLVIVAESVEKPGNLGAILRTADAAHVDAVLVCDPRVDTWNPNTIRASRGAV
                     FAVPVVEVKSAEALSWLRSRKMRVLAATPSADAMYTDVDMQGPLAIAVGTEDEGLTDF
                     WMQNSDLRVKIPMTGKVNSLNVSIATALMTYEAVRQRAK"
     gene            2124..>2380
                     /locus_tag="PLT08_14235"
     CDS             2124..>2380
                     /locus_tag="PLT08_14235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE05681.1"
                     /translation="MSSKPARQNSRSETAVFANLGRDLASTTEARSAAEIILNAADSL
                     IGWDAAYLILYDPAIGGVPRPLLTIDTINGNHVEQKNAMPQ"
BASE COUNT          606 a          590 c          571 g          613 t
ORIGIN      
        1 gttggcagtt atctttgtga cccacgatct tggtgttgtt gccgagttat gtgataaagt
       61 gcttgttatg tatggcggtg tgaccgctga atatgctgat gtagacgtaa tctacaattc
      121 tccgcaacac ccctataccc aagagctttt gaaagccttt ccagatttaa cgaagccaaa
      181 gaaaaaactc tcttctattc cgggctaccc gccgcgttta gataacctgc cgcaaggctg
      241 tcgctttgct ccgcgctgcc cagcagcctt tgatcgttgc catacagaag agccccttct
      301 tcataccatc gcaaacgggc atgtagtgag ctgccacctg attgagaaat caaaatgaag
      361 aatactaccg aaaaactttt ggaaattcgt ggtctgaaaa aatattttga agcggggcgc
      421 ccatccttgt tttcacgcga aacgaaacat gtccacgctg tggatggtgt gaacctgacc
      481 ttacgtcgca gcgaagtaat tgccctcgtt ggcgaaagcg gatgcggcaa atcgaccttg
      541 ggcttgttgc tgatgggttt ggaagacccc actgacggct cggttatttt tgaagatcgc
      601 gatattacac atctcaacga ccatcaacga aaagacctgc gccgaaaaat tcaaatggtt
      661 tttcaagacc cctatgagtc tttgaacccc acgcagacca ttgaagaaat cgtaaccgaa
      721 ccgttgaccg tgcatggtat ggcatcatct catcaagacc gtgatgaacg tgttcgaaaa
      781 gccatggaag acgccgggct aaagcctgca gatgtatatt tgcatcgtta cccgcacgaa
      841 ctttcagggg ggcaacgtca acgcgtggtc attgcatctg cgcttgtgct tgagccagag
      901 atcattattg ccgatgaacc cgtgtctatg ttggatgtat ccatccgcgc agaagtgatc
      961 aaccttctgg cagagcttcg catctcgcgt cagatcgcag tcatttttat tacgcatgat
     1021 cttggctcgg ttggcttctt tgctgaccgt gtagcagtga tgtatctcgg acgaatcgta
     1081 gagattggta caatgttaga agttcttgag aaaccacagc acccgtatac caaagcgttg
     1141 ctctcggtta tccctgtacc caatccgcgt ttacggcatg aacgcattgt ccttcaaggt
     1201 gaaacgccca accccattaa tatcccttcg ggttgtcgtt tccatccgcg gtgcccggca
     1261 gcctttgatg cctgcaaact cagtgacccg cccatgacaa ccctttcaaa atcacatcag
     1321 gcagcttgtc tgttagtaac gaatacaaaa taaatggata ttacaagttt acaaaacccg
     1381 cgcgtcaagc atattgtcaa attgcgtgag gataaaaagc agcgccaaaa ggatcgcttg
     1441 attctggtgg aaggctacga tgaattgact ctcgcgcttt cgtgtggatt cgaaccgcaa
     1501 acgcttcttt ccgcgcccga gcttgttagc caatccctaa ctgttcccaa cgctgaaaat
     1561 ttgacagtga gccgtgccgt ctttgaaaag atttcctacc gtgataaccc cgatggttgg
     1621 cttgggcttt tccctatgcc aacatccagc ttggatgaat tgaagctttc agaaaatccg
     1681 ctagtgattg tcgctgagtc tgtagagaag cctggtaatc ttggcgccat ccttcgcact
     1741 gccgatgccg cgcatgtaga tgcagttctc gtctgcgacc cgcgtgtgga tacgtggaat
     1801 ccaaacacca ttcgcgccag ccgcggagca gtctttgccg tacctgttgt cgaggtgaaa
     1861 tcggctgaag cgctgagttg gttgaggtcc cgtaagatgc gtgttctggc tgcaactcca
     1921 tctgccgatg caatgtacac agatgttgat atgcaaggtc cgcttgccat tgcggttggg
     1981 acagaagatg aaggtctcac cgatttctgg atgcagaatt cagacctgcg tgtaaaaatt
     2041 cccatgacgg gaaaagtaaa ttccttgaat gtttcgattg ccactgcact aatgacttat
     2101 gaggcagtac gacaaagagc aaaatgagca gcaagccggc acgtcagaat tctcgaagtg
     2161 aaacagccgt ttttgcaaac ttggggcgtg atctagcatc gacgactgaa gcaaggtctg
     2221 cagcagagat catattaaat gccgcagata gccttattgg ttgggatgcc gcttatctta
     2281 tcctttatga ccctgccatc ggtggtgttc cacgcccatt gcttacaatc gacactatta
     2341 atggaaacca tgtagaacaa aagaatgcca tgccgcaaaa
//
LOCUS       DAOQJE010000617         2379 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_33170_length_2379_cov_3.187177, whole genome shotgun sequence.
ACCESSION   DAOQJE010000617 DAOQJE010000000
VERSION     DAOQJE010000617.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2379)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2379)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2379
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33170_length_2379_cov_3.187177"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..394)
                     /locus_tag="PLT08_14240"
     CDS             complement(<1..394)
                     /locus_tag="PLT08_14240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE05682.1"
                     /translation="MPEDDVFNHEQGGETPPLRGRTIYNLTMNKFDPQKHHRHSIRLK
                     GYDYSQEGAYYVTIVTWQREFLFGEIVNQEMMLSPYGEIVQKWWGEIPVHFPNVETGA
                     FVIMPNHVHGINYILDGRGMETVPEEDGV"
     gene            complement(424..1290)
                     /locus_tag="PLT08_14245"
     CDS             complement(424..1290)
                     /locus_tag="PLT08_14245"
                     /inference="COORDINATES: protein motif:HMM:NF013704.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006306 - DNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methyltransferase"
                     /protein_id="HNE05683.1"
                     /translation="MFNSDNEDFNKEKSTVWNFPVRGAWATHKPDYRGNFAPQVPRNV
                     ILSYSDEGDLVLDPMVGGGTTLIEARLLNRNAVGYDVNQNAVNITSERIRFEMKGNTK
                     QVVKLGNAQKLPEKDNSVDLVIAHPPYANIVKYSDGKNPDDLSSISSLPKFLDALEIA
                     VREMYRVLKPGKYCAILIGDTRKGQHFIPLSHFVLERCLRSGFALKEEVIKTQHNTTH
                     APRWSASAKHYKFYLIMHEHLFIFRKPDVGENLTRIQYSTWKGLQKAQATEEEEEWKG
                     LLDWENKKGKGK"
     gene            complement(1501..2226)
                     /locus_tag="PLT08_14250"
     CDS             complement(1501..2226)
                     /locus_tag="PLT08_14250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE05684.1"
                     /translation="MKQHEAVILAMKQNGGYATLGQLYQVAPKIAGSKWGTKTPFASI
                     RRIVQQHDDFFKIRPGLWALTSEKERVLRLFSGEEAKPKEREYSHYFYQGLVAEIGNL
                     QGFKTYVPPQDRNKPFAEQKLGSVTTVSEFYEFTYSDVLRRAKTIDVTWFNDRKYPSS
                     VFEIEHSTDIQNSLLKFVELQDFRAKFFIVADAKRKAEFDSKISFYAFSAIKSFVKFW
                     DYDSVLDIHAKVAASNLAVKEIL"
BASE COUNT          673 a          561 c          401 g          744 t
ORIGIN      
        1 ttacaccatc ttcctcgggc acggtttcca tgcccctacc atccaagata taatttattc
       61 catgcacatg attcggcata attacaaatg cccctgtctc cacattcgga aaatggacag
      121 ggatttcgcc ccaccatttc tgaacaatct ccccatacgg actcaacatc atctcttgat
      181 tcacaatttc cccaaataaa aattccctct gccatgtcac aatcgtgaca taatacgcac
      241 cttcttgtga ataatcatat cccttcaacc gaattgaatg tctatgatgt ttttgtgggt
      301 caaatttatt catagtcaaa ttataaatgg tgcgaccacg taggggcggg gtctccccgc
      361 cctgttcatg attgaagaca tcatcctcgg gcacggagac cgtgccccta cctaccaaag
      421 attttattta cccttcccct tcttattttc ccaatcaagc aaccccttcc actcttcttc
      481 ctcttcggtg gcttgcgcct tctgcaagcc tttccacgta ctgtattgga ttcgggtcag
      541 gttttcccca acatcgggtt ttctaaaaat gaaaagatgt tcgtgcataa tcaagtaaaa
      601 cttgtaatgc tttgcgctcg ctgaccatct tggcgcatga gttgtgttgt gctgggtctt
      661 gataacttct tccttcaatg caaatcctga acgcaagcat cgttccaaga caaaatgtga
      721 caacggaata aagtgctgtc ccttccttgt atcgccaatc aaaatggcac aatactttcc
      781 tggctttaaa acgcgataca tttcccgaac agcgatttcc agggcgtcca agaattttgg
      841 caagctggaa atgctggata aatcatcggg atttttaccg tccgaatatt tcacaatatt
      901 tgcataaggc ggatgagcga tgaccaaatc aaccgagtta tctttttcag gcaatttctg
      961 agcatttcca agtttgacta cctgcttggt gttacccttc atttcaaagc ggattctttc
     1021 agacgtgatg tttacagcat tttgattaac atcatatcca acagcattac gattcaacaa
     1081 ccttgcttca atcaaagttg tgccacctcc aaccattgga tctaaaacca agtcgccttc
     1141 atcggaataa ctcaaaatta catttctggg aacttggggc gcaaaattcc cgcgatagtc
     1201 tggtttatga gttgcccatg cgccacgaac tggaaaattc caaactgtac ttttttcttt
     1261 attgaagtct tcattatctg agttaaacat atttttctca cgaaccatta ttggcaacct
     1321 cgtttttaat ttgtgataag caataattta gcaggtcaaa actcaaaaca caacctgatt
     1381 taagtgacat tgctaaacct ttattagatc gcggatttta ctggttaaat aaaacaggtt
     1441 gtgaccgctg acagtcacaa cctgaaattg ataaaaactg cttttaggtg tctcgcaaca
     1501 tcataatatt tccttgactg caagattact tgccgctact ttggcatgta tgtctaacac
     1561 cgagtcgtaa tcccaaaatt tcacaaaaga cttaatggca ctaaaggcat aaaaagaaat
     1621 tttgctatca aactctgctt ttcttttggc gtcagcaaca ataaaaaact tcgctctgaa
     1681 atcttgtaac tccacaaact tcaacaatga gttttgaata tcggttgagt gttcaatttc
     1741 aaaaaccgaa cttggatatt ttctatcatt aaaccaagta acatcaattg tcttggctct
     1801 tctcaatacg tctgagtaag taaattcgta aaactctgaa actgttgtaa cacttcctag
     1861 tttctgttct gcaaaaggct tgtttctatc ctgaggagga acataagttt taaagccttg
     1921 taaatttcca atttctgcaa ccaaaccctg ataaaagtaa tgtgaatatt ctctctcttt
     1981 tggttttgct tcctcgcctg aaaacaagcg aagaaccctc tctttttcag atgttaatgc
     2041 ccacaatcct gggcggattt tgaagaaatc atcatgttgt tggacgattc ggcgaatact
     2101 cgcaaaagga gtctttgttc cccacttact gccagcaatt ttcggagcaa cctgatacaa
     2161 ctgtccaagt gttgcgtaac caccattttg tttcatcgct aaaattacgg cttcatgttg
     2221 cttcatggaa tttacctcgc tcaacacatg aaagaattta ctctgctagt cattataaat
     2281 gatcgcacga agttattcct cgccaaacag ttttgagaca gagactttca atgcttgtgc
     2341 aattgttcgt gtttctatat ccgtcaaatg cctatcacc
//
LOCUS       DAOQJE010000618         2377 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_33233_length_2377_cov_6.407838, whole genome shotgun sequence.
ACCESSION   DAOQJE010000618 DAOQJE010000000
VERSION     DAOQJE010000618.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2377)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2377)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2377
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33233_length_2377_cov_6.407838"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1314)
                     /locus_tag="PLT08_14255"
     CDS             complement(<1..1314)
                     /locus_tag="PLT08_14255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HNE05685.1"
                     /translation="MFDLPDNEILPLSKEHVFWTWSAQAKVNPIAVKRAKGVYFWDVD
                     DKRYLDFNSMTMCVNIGHGDERIIKAMQEQAAELPYAAPGMTTKIRALASREVASVTP
                     QGKLSKVLFTLGGADANENAIKLARGYTKRHKILTRYRSYHGASAGAMAATGDPRRVA
                     WEPNLMTGVVHFLDPYRYRSTFHRTNPNISEADFAKDYVNHLEEIILYEGPESIAGIL
                     MESVTGTNGIIIPPDGYMQGVRTLCDKYGIVMICDEVMSGFGRTGKWFAIENWNVVPD
                     MITMAKGLTSAYAPLGAVAMKPEIAAAFDEHAFESGLTYTSHPISLAAAVANIQAMRE
                     DKVVEHAASMGGVLRRMLSDLGEAHPSVGEVRNIGLFGIIELVKNRETKEPMVPWNGS
                     SPEMVALKKYCSDHGLFLYTHWHTVLIIPPLIINEKELQEGFDVLD"
     gene            1391..1948
                     /locus_tag="PLT08_14260"
     CDS             1391..1948
                     /locus_tag="PLT08_14260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE05686.1"
                     /translation="MNTFTYKRIVIIGTTSSGKSTLAEKLSTILGVNFIELDALHWEP
                     NWKEADTLVFRQRVDLATQAIGWVVAGNYSAVRDIVWNRAEVVIWLDYSFHIVFWRLL
                     TRTIRRAVTQEVLWNGNRESFWWHLKLWSEESLFHWLFKTYWRRKREIPILLSKPENR
                     HLTILHFRHSQEAEAWLKGLSPTNA"
     gene            complement(1931..>2377)
                     /locus_tag="PLT08_14265"
     CDS             complement(1931..>2377)
                     /locus_tag="PLT08_14265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE05687.1"
                     /translation="CRFYTNGKICVGGKLLFIALIVCILISTGSFVWGYSQAGYEDAV
                     RWLVAFGVLWFLAEWRKWKWFSALAVLFSLFLAGYGVWFKFVPGLMFNGAVFSVLAWN
                     ITEFQEKLRLLPQREDKQGMTRRHLIRIGTLSIGAVLIALFLGIRG"
BASE COUNT          666 a          624 c          481 g          606 t
ORIGIN      
        1 atccaaaaca tcaaagcctt cctgcaattc tttttcgtta ataatcaacg gcgggatgat
       61 caaaaccgta tgccaatggg tatatagaaa caagccatga tcggaacaat attttttcaa
      121 ggcaaccatt tctggcgaag acccattcca tggcaccata ggttctttcg tctcacgatt
      181 ctttaccaac tcgatgatac caaatagacc aatattccgc acctcaccca cagatggatg
      241 cgcctcaccc aaatcggaca acatacgcct caagactccg cccatgcttg ctgcgtgttc
      301 tacaacctta tcctccctca tcgcttggat attcgccaca gctgccgcca atgaaatagg
      361 atgagatgta tacgtcagcc cgctttcaaa ggcatgctcg tcaaatgcag cagcaatctc
      421 tggcttcatt gccacagccc cgagaggggc atatgccgat gtaagcccct ttgccatagt
      481 aatcatatct ggcaccacat tccaattttc aattgcaaac catttgccag tgcgcccgaa
      541 gccactcatc acttcatcgc agatcatcac aataccgtat ttatcgcata acgtacgaac
      601 tccctgcatg tatccatctg gcggaatgat gataccgttc gtgcctgtca cagactccat
      661 taagatgcct gcaatagact cgggaccttc gtacagaatg atttcttcta aatggttgac
      721 ataatccttc gcaaaatctg cttcactgat attcgggttg gtacggtgaa aggtggatcg
      781 atatcgatat gggtcaagga aatgaaccac gcctgtcatc aaattcggct cccaagccac
      841 ccggcgcgga tctcctgtgg cagccatagc gccagcagaa gcaccatggt acgaacgata
      901 gcgcgttaat atcttatgac gtttggtgta tcctcgcgcc aatttgatgg cattctcgtt
      961 cgcatcggca ccaccgagcg tgaacagcac cttgctcaat tttccctgcg gtgtgaccga
     1021 tgccacttct ctactcgcca aggcgcggat tttcgtcgtc ataccgggag ccgcataggg
     1081 caactccgcc gcttgttcct gcatagcttt aatgatacgt tcatccccgt gcccgatgtt
     1141 cacacacatc gtcatagagt tgaagtcaag atagcgttta tcgtctacat cccaaaaata
     1201 cacgcccttg gcgcgtttta ccgcaatggg attaaccttg gcttgcgcag accacgtcca
     1261 aaagacgtgt tctttcgaga gagggaggat ttcattatcg ggaagatcga acatggagaa
     1321 ttccttcgca atgcgaaatt catactatgt aaaatacacg gtacgtatca cgaattataa
     1381 tcccctgccc atgaacacat tcacttacaa acgtattgtt attattggca ccacatcttc
     1441 aggcaaatct acgctagcag aaaaactctc tactatacta ggggtgaatt ttattgaatt
     1501 agatgctctt cattgggagc cgaactggaa agaagctgat acgcttgttt ttcgccaaag
     1561 agtagacctt gctactcagg caataggttg ggttgttgca gggaattaca gtgctgtgcg
     1621 cgatattgtt tggaaccggg cagaagttgt catctggttg gattattcct tccatattgt
     1681 attttggcgt ctactcaccc gcaccattcg ccgcgctgta acgcaggaag ttctttggaa
     1741 cgggaaccgt gagtcatttt ggtggcacct caaactctgg tctgaagagt ctttgtttca
     1801 ttggctgttc aaaacctatt ggcgcagaaa acgtgaaatc cccatcttgt tgtcaaagcc
     1861 tgaaaatcga cacctcacaa tattgcattt tcgccactca caagaagcgg aagcatggct
     1921 aaagggtttg tcacccacga atgcctagaa aaagcgcaat caagaccgca ccaatagaaa
     1981 gagttccaat gcgtatcaag tggcgtcttg tcataccttg tttgtcttct cgttgaggta
     2041 gtagcctcaa tttttcttga aattcagtaa tgttccatgc caatacacta aacacagcac
     2101 cgttaaacat aagcccagga acaaacttaa accaaacgcc atacccagct aaaaacagac
     2161 taaacagcac agctagagca gagaaccact tccatttacg ccattccgcc aaaaaccaaa
     2221 gaacaccgaa agctactagc cagcggacgg catcctcata gccagcttga gagtatcccc
     2281 atacgaacga gcctgttgat atcaagatgc agacgataag cgcaataaat aacaattttc
     2341 ctccaacaca aattttacca ttcgtataaa acctgca
//
LOCUS       DAOQJE010000619         2375 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_33292_length_2375_cov_4.952155, whole genome shotgun sequence.
ACCESSION   DAOQJE010000619 DAOQJE010000000
VERSION     DAOQJE010000619.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2375)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2375)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2375
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33292_length_2375_cov_4.952155"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..662
                     /locus_tag="PLT08_14270"
     CDS             <1..662
                     /locus_tag="PLT08_14270"
                     /inference="COORDINATES: protein motif:HMM:NF014740.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HNE05688.1"
                     /translation="TAVRHGVERLVLISTDKAVRPASVMGATKRLAELTVLDAAQRNN
                     LPYSVVRFGNVLGSRGSVVRTFKNQIARGGPVTITHPEMYRYFMIIPEAVHLVLQAAC
                     MGTGGEVFMLNMGEQVRILDLAEDLIRLSGLEPYRDIDIQTTGIRPGEKLREDLLEDG
                     VDFERTQHSEIFRMSKEEQVDGSALAQTLDKLSDLALHSRTSDLIQTINHFLPSGSVQ
                     S"
     gene            659..1642
                     /locus_tag="PLT08_14275"
     CDS             659..1642
                     /locus_tag="PLT08_14275"
                     /inference="COORDINATES: protein motif:HMM:NF024871.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016755 - aminoacyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0044038 - cell wall macromolecule
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan bridge formation glycyltransferase
                     FemA/FemB family protein"
                     /protein_id="HNE05689.1"
                     /translation="MTEVALAEWNQFLTKFPHTHLLQMGEWGELKKDFGWKPIRIISE
                     NVGVQILFRRLPLGFTIAYMPKVASDQLSTTSDQFWREVDSVCKKNRAIFLKLEPDTW
                     TDEFITHHSSFIISKHNIQPPRTITLDINASEENILSRMKPKCRYNIRLAEKKGVTIR
                     AWGDIPAFYEMMTVTGGRDGFGVHSKEYYQRAYELFHPKGTCELLVAEYEGKPLASLM
                     VFANAKRAWYVYGASNNEERNRMPTYLLQWEAIRWAKARGCEEYDLWGVPDEDEETLE
                     AQFESRHDGLWGVYRFKRGFGGQLKRAVQALDRVYNPLLYKIYLWRFSGGE"
     gene            1645..1932
                     /locus_tag="PLT08_14280"
     CDS             1645..1932
                     /locus_tag="PLT08_14280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE05690.1"
                     /translation="MSYFDVIQEIEKLPHYREFVCSACGYKQRVLVLIIDYKCEKCGE
                     NLKLRGHAPIGTEIEDVIDAVLEWMGKGTKLNDVMEWKNIRDSFPIDGTNE"
     gene            1925..>2375
                     /locus_tag="PLT08_14285"
     CDS             1925..>2375
                     /locus_tag="PLT08_14285"
                     /inference="COORDINATES: protein motif:HMM:NF014445.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016755 - aminoacyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0044038 - cell wall macromolecule
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan bridge formation glycyltransferase
                     FemA/FemB family protein"
                     /protein_id="HNE05691.1"
                     /translation="MSNNFWNDLISKLPNPHFLQTYEWGQVKAKYGWVPYYAVWTEDG
                     KWKVESDVNSLSTFHSPIVAASLILKRTAFRRFSIFYAPKGPLMDWTNESLRKRVLDD
                     LQSFAKKQGAIFLKLDPDVVLGRGVPASVGEVTENSGQAVMSDLRRRG"
BASE COUNT          621 a          611 c          585 g          558 t
ORIGIN      
        1 aaacagcggt caggcacggg gtagaaagac tcgtgctcat ctccaccgat aaagccgtgc
       61 gtcctgccag cgtgatgggc gctaccaaac gtcttgccga actcaccgtc cttgacgcgg
      121 cgcaacgtaa taacctgccg tattcggttg tgcgttttgg caacgtgctc ggcagtcgcg
      181 gcagtgtcgt gcgcaccttc aaaaatcaaa tcgcgcgcgg cggacccgtc accatcacac
      241 atcctgaaat gtatcgttat ttcatgatca tccccgaagc cgtgcatctt gttttgcaag
      301 ccgcctgcat gggcactggc ggcgaggtct tcatgctcaa catgggcgag caggttcgca
      361 tcctcgatct cgccgaagac ctcattcgcc tctctggtct cgaaccctat cgcgacatcg
      421 acattcaaac caccggcatt cgccccggcg aaaaactgcg cgaagacctg ctcgaagacg
      481 gcgtggattt tgaacgcacg caacactcgg aaatttttcg catgtcgaaa gaggaacagg
      541 tggacggctc cgcgcttgct caaactttgg acaaactctc agacctcgcg cttcactccc
      601 ggacctctga cctgattcag accatcaacc atttcctgcc ctccggctcg gtgcaatcat
      661 gaccgaagtg gcgcttgctg aatggaatca attcctaact aaattccccc acacgcatct
      721 cttgcagatg ggcgaatggg gtgaattaaa aaaagatttt ggctggaagc ccatccgcat
      781 tatctctgag aatgttggcg tacaaatttt gttccgccgc ctgccgctgg gattcacgat
      841 cgcgtacatg ccgaaagtag ccagtgacca gttatcaacg accagtgacc agttctggcg
      901 tgaagttgat tctgtttgta aaaagaaccg tgccatcttt ctaaaactcg aacccgacac
      961 atggacagat gaattcatca ctcatcattc atcattcatc atttccaaac ataacatcca
     1021 acctccccgc accatcaccc tagacatcaa cgccagcgaa gaaaatattc tgtctcgaat
     1081 gaaacccaaa tgccgctaca acattcgcct cgctgagaaa aagggcgtga ccatccgcgc
     1141 atggggcgac atccccgcct tctacgagat gatgaccgtc accggcgggc gtgatggttt
     1201 tggtgtacat tccaaggaat attatcaacg cgcctacgaa ctcttccacc caaaagggac
     1261 gtgtgaactg ctcgttgcag aatacgaggg taaaccgctg gcttcgctga tggtcttcgc
     1321 aaatgccaaa cgcgcttggt atgtgtatgg cgcgtcgaac aacgaagaac gcaaccgcat
     1381 gccgacatat ctcttgcaat gggaagccat ccgctgggca aaggcacgcg gctgtgaaga
     1441 atacgacctg tggggtgtcc ccgacgagga cgaagaaaca ctcgaagccc aattcgagtc
     1501 gcggcacgat ggcttgtggg gcgtctaccg tttcaaacgc ggctttggcg gtcaattgaa
     1561 acgtgccgtg caggcattgg atagagttta caatccgctt ttatataaaa tatatctgtg
     1621 gcgattttct ggaggcgaat aattatgtct tattttgatg taattcagga gattgaaaag
     1681 ttgcctcatt accgcgaatt tgtttgtagc gcatgtggtt ataaacaaag ggttttagtt
     1741 ttgataatcg attacaaatg tgaaaagtgt ggcgaaaatt tgaaattaag aggacatgct
     1801 ccaataggaa ctgaaataga ggatgttatt gacgcagtac tagaatggat gggaaaggga
     1861 acaaaactta acgatgtgat ggagtggaaa aatataagag actccttccc gattgacgga
     1921 actaatgagt aataactttt ggaatgactt aatttcaaaa ttacctaatc cgcacttcct
     1981 gcaaacctat gaatggggac aggtcaaagc gaagtatggc tgggttccat attacgccgt
     2041 ctggacagaa gatggaaagt ggaaagtaga aagcgatgtc aattcacttt ccactttcca
     2101 ctcaccaatc gtcgctgctt cactcattct caaacgtacc gctttccgtc gtttctccat
     2161 cttctacgct cccaaaggtc cgctcatgga ttggacgaat gagtccctgc gtaaacgcgt
     2221 gctggatgac ttgcaatcct tcgcgaagaa acaaggcgca atcttcctga aacttgaccc
     2281 cgatgtggtg ttggggcgcg gagtccctgc cagcgtgggg gaggtcacag aaaacagcgg
     2341 acaggctgtg atgtccgatc tgaggcgcag gggct
//
LOCUS       DAOQJE010000620         2372 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_33371_length_2372_cov_4.003021, whole genome shotgun sequence.
ACCESSION   DAOQJE010000620 DAOQJE010000000
VERSION     DAOQJE010000620.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2372)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2372)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2372
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33371_length_2372_cov_4.003021"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            399..596
                     /locus_tag="PLT08_14290"
     CDS             399..596
                     /locus_tag="PLT08_14290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE05692.1"
                     /translation="MSNSSTGQPKQVFTPEQRLALGRVYSFLIEIGRQRLTRLQTTDT
                     SKLTIEDETPNNTTETLINSE"
     gene            824..1411
                     /locus_tag="PLT08_14295"
     CDS             824..1411
                     /locus_tag="PLT08_14295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE05693.1"
                     /translation="MNHIKRLWGHYLALMILVAALAYPIYRVYRFFQTRYGQEAALIA
                     LTLTVMVVMVLVVRGAVHTHRLIRDLKRDLAKDDGHELPTGRSIPLIFKLFKINPKET
                     TEQADAEGTIKFPPLFNLSTNRHRGKPPRFPKEQIRKAVIKWESRDPSITALTLEQFL
                     AQEFGSGPDGILLMATTTFYDWRRRILKEMESGEH"
     gene            1803..2000
                     /locus_tag="PLT08_14300"
     CDS             1803..2000
                     /locus_tag="PLT08_14300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE05694.1"
                     /translation="MTTTLPTPSPSRFTPEQKEILGQVYTLIMTWRRERLRLKNNPLQ
                     TTSDMTPPTNPSPSQSGGSHE"
     gene            1993..2115
                     /locus_tag="PLT08_14305"
     CDS             1993..2115
                     /locus_tag="PLT08_14305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE05695.1"
                     /translation="MNDALQAVLIAIALIIGPMLLGSILYIVIRDAVKRNLRKD"
     gene            2127..>2372
                     /locus_tag="PLT08_14310"
     CDS             2127..>2372
                     /locus_tag="PLT08_14310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012902626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNE05696.1"
                     /translation="MSTKIICIANQKGGVGKTTTAVSLAHGLVIKGKRVLLIDLDPQG
                     QSATALGRSPEPGVFYLLTMGMSQQETAFIHSWVRFSG"
BASE COUNT          690 a          638 c          492 g          552 t
ORIGIN      
        1 cgtatgtcgt cagccagtct tccataatat atagtagcgc ctccaaacga tattataacg
       61 tcgtgaacgg agaaatcgac actggtgttc cggttgtaaa agtaccaagc agaggtatag
      121 catccgggac tccgttaact ctacggattg gtgtccgact tacgtatgac ttcgccccac
      181 acataaaaaa gaaggttttg gagggaataa actcgtaggg atatccgtat tcaaatccgt
      241 agtagaaccg tacaggcagg tgctattttt aggcatgcag ttgaatactt caatttcttc
      301 aggaaggcag ccatgcataa aatagtacct attctcaaat atcgaaaaaa taatttcaaa
      361 ttcactggac aagtatcctg ccaggaaagg ggaagatcat gagcaattct tcaacaggac
      421 aacccaagca agtatttaca ccagagcaaa ggcttgcctt agggagagtg tatagttttc
      481 tgattgaaat tggacggcaa cgattaaccc gtcttcaaac aacagacaca tcaaaactca
      541 ccatagaaga tgaaacgcca aacaacacaa ccgaaactct aatcaattct gaatgaggtc
      601 cttccattga aggaacacag caggttgtgt aaacaagatt tggtctacac aacctgctgt
      661 actattctat gagaagttct caagcagatt tattttcaat tatttccact tcgaaattga
      721 aatgagttaa atcccatcaa aatagtgcca aatatcagtt gtcgaaaact tccctagttc
      781 tctaaaatac cccgccctta ctttaatatt tgaggttaca acaatgaatc acatcaaaag
      841 gctttgggga cattaccttg cgttaatgat cctcgttgcc gccctcgcgt atcccatcta
      901 ccgggtctac cgattttttc aaacccggta tggacaggaa gcagcactaa tcgcactcac
      961 cctgaccgtc atggtcgtta tggttttagt ggtacgggga gctgtgcata cccatcgatt
     1021 gatacgcgat ctgaaacgtg atctcgccaa ggatgatgga catgaactcc caacaggtcg
     1081 atctatccct ttgatcttca aactcttcaa gatcaatcct aaagaaacaa cagagcaagc
     1141 ggatgcagag gggaccatca aatttccacc acttttcaat ctctccacca accgacatcg
     1201 cggaaaaccg ccacgcttcc ccaaggagca aatccgcaag gcagtcatca aatgggaaag
     1261 ccgtgaccct tccattactg cacttaccct cgaacaattc cttgcacagg aatttggcag
     1321 cggaccggac ggtattctac tgatggcgac caccaccttt tacgactggc gccgcaggat
     1381 attgaaagag atggagtccg gtgaacatta actttaagca gataaacctc attcccaatt
     1441 gaatcattcc aactgaatta tcggctcaag tatcaaccca aaaggcaaag acaattttcg
     1501 tagaattccc gtacttcaaa ccgtagagat cccgtactcc cagatgctat ttatatgagc
     1561 cgccgacaac gcggctcatt tcttttcagc atcaaaagga gacctatggc taagatcatt
     1621 gtctatctgg gggaccagga acgcaacgca ttacaccaac ttgcccaacg ggaattgcga
     1681 ttgccccggg cacaggcggc tctcatcatc cggcaggaac tggtacgact gggcatgcta
     1741 tccctgcaat cgccggcaac cgaaaccgac tccccacaca acgcacgaac gggagtctca
     1801 tcatgaccac aaccctccca accccctccc cgtcccgctt cacacctgag caaaaggaga
     1861 tacttggtca ggtctacacc ctgatcatga cctggcgccg ggaacgcctg cgattgaaga
     1921 acaacccatt gcaaaccaca tccgacatga cccctcccac caatccttca cctagtcaaa
     1981 gtggaggctc ccatgaatga cgcactgcag gcagttctaa tcgccatcgc gttgatcatc
     2041 ggtcccatgc tacttggttc cattttgtac atcgtcatcc gcgacgccgt caaacgcaac
     2101 ctgcgcaagg attaggggag atcgccatgt ccaccaaaat catttgcatt gccaatcaaa
     2161 aaggcggcgt gggcaagact accaccgcag tctccttagc acatggattg gtcatcaaag
     2221 gcaaacgggt gctcctgatc gatctcgatc cacagggaca atctgccact gccttgggac
     2281 gcagcccgga accgggagtg ttctatttgc ttacaatggg catgtcacaa caggagacag
     2341 ctttcatcca ttcctgggtg cgcttctccg gt
//
LOCUS       DAOQJE010000621         2371 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_33399_length_2371_cov_5.414076, whole genome shotgun sequence.
ACCESSION   DAOQJE010000621 DAOQJE010000000
VERSION     DAOQJE010000621.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2371)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2371)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2371
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33399_length_2371_cov_5.414076"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            212..1567
                     /locus_tag="PLT08_14315"
     CDS             212..1567
                     /locus_tag="PLT08_14315"
                     /inference="COORDINATES: protein motif:HMM:TIGR00792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNE05697.1"
                     /translation="MSNSERLSTRFKMLFSTGDLSTSIPLAILMFFQLYFLTDVARLR
                     PDYAGWAIALPRLWDAINDPLFGLLSDRLRTRWGRRRALLLFGSVPLGLSFILMWIVP
                     PFGQLGLAIYYALTFIIFDTAFTVVHVGYNSLTPELTSDYDERSSLNGYRMVFSISGT
                     LGAIILATVLGWFITDARLLFIILGVGLGLASIIPPLVVFNITHEKPADEQPEPLSWW
                     QSMQLTLSNQPFRMVMGLYLLSWTTASILAAILIYFANYRLRVPEQANYFVLLAEGAA
                     IFFIPFWVWVAKKLDKRRAFIIGTLTWIFVLLGISALPADAIGLAYVLAALSGSGIAA
                     AYVLPWSMIPDIIEHDQLQTGQRREGSYYAFASFFQKLATAFALWAMGQALAANGYIT
                     PNSSGPLPTQPDSAIQAIRFFAGPAPAVLLILAVLFAWNYPITRESHSETLRVLAERE
                     S"
     gene            1577..1885
                     /locus_tag="PLT08_14320"
     CDS             1577..1885
                     /locus_tag="PLT08_14320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE05698.1"
                     /translation="MTEKHTSCSICSNLKEYQVATEYIVSPEENINFPENVLTAIGKL
                     KKVKEISSSTDIFQCPECGTYYFYRASYEYLVSGSGSYDEYVLTREDDEIVKKYLQDD
                     "
     gene            1897..2184
                     /locus_tag="PLT08_14325"
     CDS             1897..2184
                     /locus_tag="PLT08_14325"
                     /inference="COORDINATES: protein motif:HMM:NF020496.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1905 domain-containing protein"
                     /protein_id="HNE05699.1"
                     /translation="MIIAFKGKIFQWRGPAPYLFVAVPEKESANIKAVSKLVTYGWGV
                     IPVVAKTGKTEWKTSLFPKDGRYLVPIKMVVQKAEKLKEGDEIKLQLELNL"
BASE COUNT          605 a          556 c          542 g          668 t
ORIGIN      
        1 caaagacggc gaagtctttc agcaaagttt tcgtcaacta cgcgacctga tgaaatgggc
       61 aatggagaat caatatcagg tggaagcaga agcccggtat gagtatacag acccaagtac
      121 gggcgagagg cgggttttac acgtcccaca agaattggaa caatggtaga tcaaaaatac
      181 tttgcccgcc atctaaagga gcagacattc catgagcaat tctgagcgtt tatccactcg
      241 tttcaaaatg ttgttcagta ccggggatct aagcacaagt attccgcttg cgatcttgat
      301 gttctttcaa ctttattttc taaccgatgt tgcccgcctg cgaccggact atgccgggtg
      361 ggcaattgct ctgccgcgtt tatgggacgc catcaacgac ccactttttg ggttgctctc
      421 agaccggttg cgcactcgtt ggggacgccg ccgtgcctta ttactttttg ggtctgtacc
      481 actcggttta agttttatct tgatgtggat cgtgccgccc ttcggtcaac ttggattggc
      541 gatttattat gcgttgacct ttattatctt tgatacggca tttactgtcg tccatgttgg
      601 ctacaactcg ctcacacctg agctaacttc tgattacgac gagcgtagtt cattgaacgg
      661 ctatcgcatg gttttctcga tcagtggcac attgggtgct attatccttg caaccgtttt
      721 aggttggttc attaccgatg cgcgtttgtt attcatcatt ctcggcgtag ggctgggttt
      781 ggcttcgatc attccgcctt tggttgtttt caacatcact cacgaaaagc cagccgatga
      841 acagcctgag ccgctttcat ggtggcagtc gatgcaattg accttgagca atcaaccgtt
      901 tcgaatggtt atggggttat atcttctctc atggacaacc gccagcattc tcgccgccat
      961 cttgatttac tttgcaaact atcgtttacg tgtgccagag caagcaaatt attttgtatt
     1021 gttggctgaa ggtgccgcaa tctttttcat tccgttctgg gtgtgggttg ccaaaaagtt
     1081 ggataagcgc cgtgccttca tcattggcac attgacttgg atattcgtcc tgcttggcat
     1141 ctcagctctg cctgccgatg ccattgggct tgcatatgtg ctcgcggctc tttccggatc
     1201 tggcattgcc gctgcatatg ttttgccttg gtcaatgatc ccagacatta tcgagcacga
     1261 tcagctccaa accggtcaac gccgtgaggg gtcttactat gccttcgctt ctttcttcca
     1321 aaaactcgcc acagcttttg ccttatgggc aatgggacaa gccctcgctg caaacggata
     1381 cattacccca aattcaagcg gacctctgcc aactcaacct gattctgcca ttcaagccat
     1441 ccgcttcttt gccggacccg ctcccgccgt cttgctcatt ttggcagttc tcttcgcctg
     1501 gaactatccc atcacccgcg aaagccacag cgaaacatta agagtcctcg ccgaacgcga
     1561 atcataggga ggcagtgtga ccgaaaagca tacaagctgt agcatttgta gcaatttgaa
     1621 agagtatcaa gttgctacag agtacatcgt tagcccagaa gagaacataa attttcctga
     1681 gaacgtgttg actgctattg gaaagttaaa aaaagtaaaa gaaatctcat ccagcacaga
     1741 tattttccaa tgccctgaat gtggaaccta ttatttttac cgtgcaagtt atgaatacct
     1801 agtcagcggc agcggctcgt atgatgaata tgttttgacc cgcgaagacg atgagattgt
     1861 aaaaaagtat ttgcaagacg attaggagac accagtatga tcatcgcatt caaaggcaag
     1921 atatttcaat ggcgtggacc agccccttat cttttcgtgg ctgtcccaga aaaagaaagt
     1981 gccaatatca aagcggtctc taaactggtg acgtatggtt ggggggtgat tcccgttgta
     2041 gcgaaaacag ggaagacaga atggaaaact tccctcttcc cgaaagacgg tcgctatctt
     2101 gtgcctatta agatggtagt tcaaaaagcg gaaaagctca aagaaggcga tgaaatcaaa
     2161 ctgcagcttg aattaaatct ttaaatatta tgaacgaagc ctttgcttaa taaaccaagc
     2221 aagggcttta tttttccatt agtacgagtg atacaattat caaacttatt gttatgcata
     2281 accctgaaat aatcgccaac gagaaagaag aattcaaaca agcggtgttg aacgcgcgtc
     2341 cgttcaagcc gctttatgtt aagttcaagg t
//
LOCUS       DAOQJE010000622         2370 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_33451_length_2370_cov_2.391793, whole genome shotgun sequence.
ACCESSION   DAOQJE010000622 DAOQJE010000000
VERSION     DAOQJE010000622.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2370)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2370)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2370
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33451_length_2370_cov_2.391793"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            166..825
                     /locus_tag="PLT08_14330"
     CDS             166..825
                     /locus_tag="PLT08_14330"
                     /inference="COORDINATES: protein motif:HMM:NF017833.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exopolysaccharide biosynthesis protein"
                     /protein_id="HNE05700.1"
                     /translation="MNHIELSSKEESLLGQETLAEKMELVVEKLPADEVTLVEIMDIV
                     GADSLMLVTIFMSLIFLVPVSIPGVSTVFGTGILLIGITHLFARKLWLPKPIANRKLS
                     ASKLKEAFRRAIIWLHRLEKVSRPHRLSKLTAEGGMTIFNNLSFILAALLLMAPFGFI
                     PFSNTLPALALIFFALGMMERDGGSIIFGHISNIATIIYFGFLIAGGGWSISEFIKAL
                     G"
     gene            867..1229
                     /locus_tag="PLT08_14335"
     CDS             867..1229
                     /locus_tag="PLT08_14335"
                     /inference="COORDINATES: protein motif:HMM:NF037970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HNE05701.1"
                     /translation="MKYIAILFTLFIITIIILADKNALPPFVRAIYDFPNGDKFGHFI
                     LFGLLSFILTLTFLRTLPNQARSRVALSVGLTLAVAITLEEFSQRYFSARTFDLIDLT
                     ASYLGLLIGGWIAFRLKK"
     gene            complement(1262..>2370)
                     /locus_tag="PLT08_14340"
     CDS             complement(1262..>2370)
                     /locus_tag="PLT08_14340"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013927.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016811 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear amides
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="penicillin acylase family protein"
                     /protein_id="HNE05702.1"
                     /translation="AQNLLYADVEGNIGYQMPGDIPIRKNGDGTLPVPGWTDEYEWEG
                     FIPFEELPYALNPEEGYIVTANNQVNPWDYPYMITKDWDYGYRAQRIVDMIKNAPGKI
                     DKEYIQSMQGDATDLNAASIVPVWKEIDYKAATENEAYAIDLMQNWDYTCKADSQACA
                     VFQMFWWHLDQNTINDDVPERNAVSGGSKDFETFRHLLPEPDNFFWDDKLTTDVTETR
                     DDIFIASLTEAVQQFEKEYGKDPANWPRWGELHTATFRNGTLGESGVSLIEDLFNRGP
                     FETNGGQSLVNATGWDLGTSFEVDWLPSMRMIVDLSNLNESLTVHTTGQSGHAYHPHY
                     DDMALFWADVSYYPMWWDQESIIDDAEGHLVLKP"
BASE COUNT          580 a          552 c          522 g          716 t
ORIGIN      
        1 atatcccctt tgtgaaaaag aattttgagt tggtcattat tgccatcatt ttgatttcga
       61 tcgtaccaat gatctacgaa tggtggaagg cacgtcgcgc aacaaaacaa acagcgtaat
      121 gttctgaaag cattctttac tttagaaaat actgaggata ttaaattgaa tcacattgaa
      181 ttatcttcca aggaagagtc gttacttgga caagaaactc ttgccgaaaa aatggaattg
      241 gtcgttgaaa aactgcctgc tgatgaagtg accttggtag agattatgga cattgttggt
      301 gccgatagcc tcatgttggt aaccatcttc atgtccttga tttttctagt tccggtttcc
      361 attcctggtg tcagtactgt tttcggtaca ggcatactct tgattggcat tacgcatctc
      421 ttcgcacgaa agctttggct acccaagcct atcgcgaatc gcaaactctc ggcgtccaaa
      481 ttaaaagagg cattcagacg tgccattatt tggttgcatc gcttggaaaa ggtcagccgc
      541 ccacatcgct taagcaaact cacagcagaa ggcggcatga ctattttcaa taatttgagt
      601 tttatccttg ccgcgctttt gctaatggca ccttttggat tcatcccgtt cagcaatacc
      661 cttcctgctt tggcattgat tttctttgcg ctcggtatga tggaacgaga tggtggctcg
      721 atcatttttg ggcatatttc caacattgcc acgatcatct actttggctt tttgatcgct
      781 ggcggcggat ggtctattag cgaatttatc aaagcacttg gctagctcgt gaggcaggct
      841 gggactcaag cccagcctgt ttttttatga aatacattgc gattctgttt acacttttta
      901 tcatcacgat catcattctt gcggacaaaa atgccttgcc gccttttgtt cgtgcgatct
      961 acgattttcc caatggcgat aagtttgggc acttcatcct ttttggactc cttagcttta
     1021 ttctcactct cacttttctc cgcacgcttc ccaaccaagc ccgcagccgg gttgctcttt
     1081 cagttggctt gacactcgct gtcgccataa cgctagaaga attctcgcaa cgttacttct
     1141 ctgcccgcac cttcgatttg atcgacctga cagcaagtta tctcggtttg ttgataggtg
     1201 gatggattgc gtttcggtta aaaaagtagg tcacgctcga agcgtgacct acttttttat
     1261 tttagggctt caacaccaga tgcccttcgg catcgtcaat gatggattct tgatcccacc
     1321 acatggggta gtacgataca tctgcccaga acagagccat atcatcataa tgcggatgat
     1381 acgcatgacc agactgccct gttgtgtgga ctgtgagaga ctcatttaga ttgctcaaat
     1441 ccacgatcat acgcatggac ggcaaccaat ccacttcgaa ggaggtgccc aaatcccagc
     1501 cggtggcgtt gacaaggctt tgaccaccgt tggtctcgaa aggtccacgg ttgaagagat
     1561 cttcgattaa ggaaactcca gattcaccga gtgtgccatt tctaaatgtg gcggtgtgca
     1621 attcgcccca acgtggccaa tttgcggggt cttttccgta ttctttttca aattgctgaa
     1681 cagcttcagt taatgacgca atgaaaatat cgtcacgagt ttcagtcaca tcggttgtaa
     1741 gtttatcgtc ccagaagaag ttatctggct ctggcaaaag atgacgaaag gtttcgaaat
     1801 cttttgagcc accgctgaca gcgttgcgtt cggggacatc gtcgttgatc gtattctgat
     1861 ccaaatgcca ccagaacatt tgaaatacag cacaggcttg cgaatcggct ttacaggtgt
     1921 aatcccaatt ttgcatcaag tcgattgcat aggcttcgtt ttcggtagcg gctttgtaat
     1981 caatctcttt ccaaacagga acaatgctag cggcatttag gtctgtcgca tcgccctgca
     2041 tggattgaat gtattcctta tcaatcttgc cgggtgcatt tttgatcatg tctacgatgc
     2101 gctgggcgcg ataaccatag tcccaatctt tggtaatcat gtaaggataa tcccacggat
     2161 tgacttgatt attggcagtg acaatatagc cttcttcagg attgagggcg tatggtaatt
     2221 cttcgaatgg aataaaccct tcccattcat actcatctgt ccaaccggga acgggcaaag
     2281 ttccatcgcc gttcttacga attggaatat cgcctggcat ttgataaccg atgtttcctt
     2341 ctacatcggc atacagcaag ttctgagcag
//
LOCUS       DAOQJE010000623         2366 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_33535_length_2366_cov_5.257464, whole genome shotgun sequence.
ACCESSION   DAOQJE010000623 DAOQJE010000000
VERSION     DAOQJE010000623.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2366)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2366)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2366
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33535_length_2366_cov_5.257464"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..956)
                     /locus_tag="PLT08_14345"
     CDS             complement(<1..956)
                     /locus_tag="PLT08_14345"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNE05703.1"
                     /translation="MSPRQKSKLQIDLVIPVYNEAEAIRQTYADIRKVVDALPYDFRF
                     IYVDDGSSDGTADTLRGISTDDPRVTLLQLSRNFGHQAALTAGMDASQGDVMISMDGD
                     GQHPPALIPKMISLIEQGYDVVQGQRLEEERAASFKKVTSNFFYWLINRISGTQVLQG
                     AADFRALSRNALNGVRSMHEYHRFLRGMISWMGYNSVILPYHEPDRIAGKSKYSLGKM
                     VRLASDAIFSFSLAPLYIGLSAGFVFFILAGVQLIYVLSLWLTGNTQRVEPGWSSLMG
                     VLLIASGIIMILLGFIGVYVGYIFQEVKRRPIYLVKGRTSDAR"
     gene            complement(1020..>2366)
                     /locus_tag="PLT08_14350"
     CDS             complement(1020..>2366)
                     /locus_tag="PLT08_14350"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013434.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase/oxidase"
                     /protein_id="HNE05704.1"
                     /translation="KFMGWLDKPSERGSIIIKLGLMMYDAYTGSQRTVPRHTFYSRTE
                     SLTKWNKLNPEIVNTAVYYDGLISNPERLALEIMLDAETENSNARALNYVSLVSGSGK
                     NVILRDELTDATVEIQPKLVINAAGPWIDFANHSLGVTTKFIGGTKGSHLVLDHPELR
                     KAIGDNEFFFENKDGRIVLIFPLHDRVLIGTSDIKIDHPDDARCTDEEVDYFLELTAR
                     VFPNIKVTREHVVFAFSGVRPLASSMAKTTGQYSRDHHIEVLSGDWTNLSFPVYSLVG
                     GKWTSFRAFSEEVADKSLQFLGIKRNKGTQELPVGGGRNYPRMSEERTKYINGLAAWT
                     GLSHERLETLFERYGTRAESIAEFMKVATDAPLNSLPSYSKRELLFLIQYEKAEHLDD
                     LLLRRTMLAMLGKLTRDAVIECSMLMADALNWNLERRNAEAERTLRVLADRHRVRL"
BASE COUNT          636 a          606 c          569 g          555 t
ORIGIN      
        1 cgggcatctg aagttctccc cttcacaaga taaattgggc ggcgctttac ttcctgaaag
       61 atatagccaa catacacgcc aataaaaccc agcaagatca tgatgattcc gctagcaatc
      121 agcagcacac ccatcaatga actccaaccg ggctccacac gttgtgtatt gccagtaagc
      181 cacaaagaga ggacataaat caactggacg cccgctaaga tgaagaagac aaatccagca
      241 cttaaaccaa tatacagtgg tgcaagggag aacgaaaaga tggcatctga agccaagcgc
      301 accattttcc cgagtgaata ctttgattta cccgcaatgc ggtctggctc atgatacggc
      361 aggatgacac tgttatatcc catccatgaa atcatgccgc gcaagaagcg atgatattcg
      421 tgcatagaac gtacaccatt caaggcatta cgcgacaggg cgcgaaaatc agcagcgccc
      481 tgcaaaacct gtgtaccgct gatgcggttg ataagccaat agaaaaaatt agaagtaact
      541 ttcttgaacg aagcggcacg ctcttcttca agacgctgcc cctgtaccac atcgtagccc
      601 tgttcgatca aactgatcat cttgggaatc aaagcaggtg ggtgctgtcc atcaccatcc
      661 atcgagatca tcacatcgcc ttgcgaggcg tccataccag cagtcaacgc ggcttggtga
      721 ccaaagttgc ggctgagctg gagcagagtc actcttgggt cgtcagttga aatccctcga
      781 agcgtatccg ctgtcccatc gcttgagccg tcatccacat agatgaatcg aaaatcgtac
      841 ggcagcgcat ccaccacttt gcgaatgtcc gcataggttt gccgaatggc ttcagcttcg
      901 ttataaacag gaatgacaag gtcgatctgg agtttggatt tttgacgtgg agacatcagg
      961 aagattctac cacgcagaca aaaacaccgc cgataacggc ggcgttcgtt tatatcgctt
     1021 cataaccgga cccggtgccg gtctgccaag acacggagcg ttctctccgc ctcggcattt
     1081 ctacgttcga ggttccaatt cagggcatct gccatcaaca tggagcattc gatcactgca
     1141 tcgcgtgtga gtttgcccaa catggcaagc atggtgcgac gcagaagtag atcatcaaga
     1201 tgctctgcct tttcatactg aatcaagaag agcaattcgc gtttggaata actcggcaac
     1261 gaattaagag gggcatcagt tgccactttc ataaactcag caatagattc ggcgcgcgtg
     1321 ccgtaacgtt cgaaaagggt ttcaaggcgt tcatgcgaaa gccctgtcca cgcggcgaga
     1381 ccgttgatgt atttggtgcg ctcttcgctc atgcgcggat aattgcgccc gccgccaact
     1441 ggaagttcct gcgtgccttt attgcgctta ataccgagga actgcaaaga cttatctgcc
     1501 acttcctcag agaaagcacg gaaggaggtc cacttgccac ccaccagtga atagaccggg
     1561 aagctcaggt tagtccaatc cccactgaga acttcaatat ggtggtcacg gctgtattga
     1621 ccagtggttt ttgccatgct acttgccagc ggacgcacgc ccgaaaatgc aaaaacaaca
     1681 tgttcacgtg tcaccttgat attcgggaaa acacgcgcgg ttagttcaag aaagtagtcc
     1741 acttcttcat cggtgcaacg agcatcgtct ggatgatcga tcttgatatc agaggtgccg
     1801 atcaaaacac ggtcgtgtaa cgggaagatc aacacgatac gcccatcttt attttcaaag
     1861 aaaaattcgt tatcgccgat cgcttttcgc aattcgggat gatcgagaac aaggtgtgaa
     1921 cctttcgtgc cgccaataaa tttcgtagtc acacccaggc tgtggttggc aaagtcgatc
     1981 catggaccgg cggcattaat gaccaacttc ggctggattt caacggttgc atcggtcaac
     2041 tcatcacgga ggatgacgtt cttgcccgat ccgctcacaa ggctaacgta attcaaagcg
     2101 cgggcatttg aattttctgt ttcagcatcc agcatgatct caagtgcgag gcgttctggg
     2161 ttagaaatca acccatcgta atataccgcc gtgttgacga tctcaggatt taacttattc
     2221 catttggtaa gggattcggt gcgggaatag aatgtgtggc gcggcactgt gcgttgactg
     2281 cctgtatagg catcgtacat catcaaccct agtttgataa tgatcgagcc gcgttcagaa
     2341 ggtttatcga gccagcccat aaactt
//
LOCUS       DAOQJE010000624         2366 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_33536_length_2366_cov_5.137603, whole genome shotgun sequence.
ACCESSION   DAOQJE010000624 DAOQJE010000000
VERSION     DAOQJE010000624.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2366)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2366)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2366
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33536_length_2366_cov_5.137603"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(28..261)
                     /locus_tag="PLT08_14355"
     CDS             complement(28..261)
                     /locus_tag="PLT08_14355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE05705.1"
                     /translation="MSDNAFLKIMTALLKHQVKKMIGDEALGAIGEEIVAIGGENLDE
                     KIYLLLSEKTTIEALESAAKRANDSFLEKIDLL"
     gene            complement(693..1568)
                     /locus_tag="PLT08_14360"
     CDS             complement(693..1568)
                     /locus_tag="PLT08_14360"
                     /inference="COORDINATES: protein motif:HMM:NF018649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1295 domain-containing protein"
                     /protein_id="HNE05706.1"
                     /translation="MNPVSKRSIIAIVIVLLIASGLALAGSQGGKTFNGLPIFAICVA
                     TAILIQWIAFIPAFAMQTEKFYDLAGSLSFLTVTTLAIVLGSAGDIRSLLLAGMIVLW
                     ALRLGSFLFLRIHAAGEDRRFREIKPNFARFMLTWTLQGLWVSFSLATALAVMTSTLQ
                     VGADVFMGVGFLVWAFGFMFEAAADRQKSAFNANPANKGKFIHTGLWAWSRHPNYFGE
                     IVLWIGVAIIALPVLRGWQWVTLISPIFITLLLTRVSGVPLLEKRADEKWGGQAEYEA
                     YKSQTSVLIPRAPKK"
     gene            complement(1731..2294)
                     /locus_tag="PLT08_14365"
     CDS             complement(1731..2294)
                     /locus_tag="PLT08_14365"
                     /inference="COORDINATES: protein motif:HMM:NF024769.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdeI/OmpD-associated family protein"
                     /protein_id="HNE05707.1"
                     /translation="MNISRTLYITNPKDWRKWLKQHHKTEAEIWLVYPRKATGVPRIE
                     YNDAVEEALCFGWIDSIIKTLDDEHTVQRFSPRKPKAKYSQANIERLRTLVAQKKVIK
                     EVAETLTEVLSAEFIIPPDILKAVQADKEAWKNFQGFSDAYIRIRVAFIDGARKRPEE
                     FKKRLRHFIEMTARNKQIGFGGIEKHY"
BASE COUNT          553 a          594 c          621 g          598 t
ORIGIN      
        1 gactctcatt ccagttggac acaataacta aagcaggtca attttttcta gaaaagagtc
       61 gttagcgcgt tttgcagcac tttctaaggc ttcaattgtt gttttttcgc ttaagagcag
      121 atagattttt tcatcaaggt tttctccgcc aatcgcaact atttcttccc ctattgcgcc
      181 taatgcctca tcacctatca ttttcttgac ttggtgcttg agtaatgctg tcattatttt
      241 taaaaatgca ttatctgaca taacccaatc cttttctagt tagacaacag cacaattata
      301 agcggaaata tctgtcatat tcccaaagca caacggcaaa agcacctcac cctgattggc
      361 ttgagcaatc ggcatgtccg cttggccctc tcctaaagga gagggtatga gttcgattcc
      421 attttgcctg atggcaattt cggtcttgaa gaatagactt gggagaagat ggttttagtt
      481 tctccccctt gggggacatc gtacccgagc ctagcgagtg gtagacggga gaggggtcaa
      541 tgtggcgtct gaaattcacg cctaactggt cattgtgaag gtacgcccac agtttggctt
      601 cggcaggggt aggttctttg cgaagttccc ttgcgcgttg atatcctttg ggcgtggttc
      661 tgcgggctgg cgtacatgaa gcctaaggat gttcacttct tgggcgctcg gggaatcagt
      721 acagaggtct gagatttgta ggcttcatac tcggcttgtc cgccccattt ttcgtcggca
      781 cgtttttcga gcagaggcac gccgctaacg cgggtgagaa gaagcgtgat aaagatgggg
      841 gagatgagcg ttacccattg ccagccgcgc agcacaggca aagcgatgat ggcaacaccg
      901 atccacagca cgatctctcc aaaataattc gggtggcgcg accatgccca taaaccggtg
      961 tggatgaact tccctttatt tgcagggttg gcattgaagg cgcttttctg tctgtctgcg
     1021 gcggcttcaa acataaagcc gaaagcccac accaggaaac cgacccccat gaaaacgtcc
     1081 gcgcctactt gcagggtgga tgtcatcacc gccagagcgg ttgccaggga aaaagaaacc
     1141 catagccctt gcagggtcca ggtgagcatg aagcgcgcaa agtttggctt gatctcacgg
     1201 aagcgccggt cttctcccgc agcgtggatg cgcaggaaga ggaaagagcc gagccgaagc
     1261 gcccagagca cgatcatccc agccagaagc agcgagcgta tatcccctgc cgagccgagc
     1321 acgatcgcca gggtagttac cgtcaaaaag gaaaggctgc ctgccaggtc gtagaacttt
     1381 tcggtttgca tggcgaaagc gggaataaat gcgatccatt ggatcaggat cgcagtggca
     1441 acacatatgg caaagatcgg aagtccgttg aacgtcttcc cgccctggct tccggcaagc
     1501 gccagcccag atgcgatcaa tagaacgatg acaatggcaa taatggaacg tttgctgacg
     1561 ggattcatga tttcctcgat acaaattttg tcttgcgtaa tacaacactt gttctgttgc
     1621 acggttctat ttttcgcgca gaatgagcga aaatgaacga tataccctga caggcacgcg
     1681 tcccccaaag gcgacagaat accgtcgcgt ttggggggcg gtgagcaggc tcaataatgc
     1741 ttctcaatcc caccgaaccc gatctgcttg ttcctggcgg tcatttcgat gaaatgcctt
     1801 aatcgcttct tgaattcttc aggtcgtttg cgcgctccat caatgaacgc cacgcggatg
     1861 cgaatgtaag cgtcggagaa tccctgaaaa ttcttccagg cttctttatc ggcttgaacg
     1921 gctttcaaaa tatcgggcgg gatgatgaac tccgcactca agacctcggt cagggtttcg
     1981 gcgacttctt tgatgacctt cttctgtgcc accagggtcc gcaggcgctc gatgttggct
     2041 tgcgagtatt tggctttcgg tttgcgcggt gaaaatcgct ggacggtatg ttcgtcgtcc
     2101 agggtcttga tgatcgagtc gatccagccg aagcacaggg cttcctccac cgcgtcgttg
     2161 tactcgatgc gcggcacccc ggtcgctttc ctggggtaga ccagccagat ctcagcctcg
     2221 gtcttgtgat gctgcttgag ccactttcgc cagtctttag ggttggtgat atagagggtc
     2281 ctggagatgt tcatatttat aaaaggataa taatacagcc gccctcatgc cgttaagggc
     2341 ggctgaaaag aacgttatct ctccac
//
LOCUS       DAOQJE010000625         2358 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_33755_length_2358_cov_5.309596, whole genome shotgun sequence.
ACCESSION   DAOQJE010000625 DAOQJE010000000
VERSION     DAOQJE010000625.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2358)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2358)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2358
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33755_length_2358_cov_5.309596"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            190..1026
                     /locus_tag="PLT08_14370"
     CDS             190..1026
                     /locus_tag="PLT08_14370"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HNE05708.1"
                     /translation="MQKFISLAQIDEIVNVIKKKISIQPVIGMILGSGLNGLADSVQN
                     PVHIPYSDLPNFPTSTVHGHVGRFVIGELEGKPVIVMQGRIHYYEGYTMGEVTLPVRV
                     MHRLGVHSLFVTNAAGGVNENFVPGDVMLITDQLNLMGMSGLNPLMGPNLDEIGPRFP
                     DMSQPYDREYCDLARKVAKQSNLTLREGVYAGLSGPSFESPADLRFLRLAGADAVGMS
                     TVPEVIIARHGGMRVLGLSGVSNKANLDGSTITTHEEVIEAGRVITPKIETIVRGVLR
                     GI"
     gene            1051..1728
                     /locus_tag="PLT08_14375"
     CDS             1051..1728
                     /locus_tag="PLT08_14375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE05709.1"
                     /translation="MVDLFKFLAEYEVLIYIVLAIGALFSFRWLFRSWREWQVAVFSL
                     EREFSARRFGRSALVSAMIVIFFCVEFFMASFIIPGLPADFFVATPTLDLISTPTGTL
                     SPELMTQFANQPQVAQPNAAGCTPGQIELTFPEAGTEIKGIIELTGTVDIPNFGFYKY
                     EIAPSGSDTWATIAAGRTVVKASSLGRWDTTALTPGDYQLRLVVIDNEGNSLTPCVVP
                     VQVIPNQ"
     gene            1745..2284
                     /locus_tag="PLT08_14380"
     CDS             1745..2284
                     /locus_tag="PLT08_14380"
                     /inference="COORDINATES: protein motif:HMM:NF024698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNE05710.1"
                     /translation="MSELQIRPTVATDLTRLMGIDHSMTSEAVWQLEVRRSTGQVAVT
                     FREVRLPREIQVPYPHNHFALADDWVKRSMMYTAIKDAEPVGYISLLERGTASTVWVL
                     DLAIDAKQRRRGVAAALLASAQAWAESRSHRRLILEVQSKNVPAIRLAQKSGFEFCGY
                     NDQYYLNKDVALYFAKVLK"
BASE COUNT          559 a          523 c          613 g          663 t
ORIGIN      
        1 gaaatttttc tcgttagggc gcttcatgtc gctagaggat agcataagaa acaaatttta
       61 tctagttcac ttcaattgtg cattttatgg ctgaaaagcc cgcagggtca tttctgttat
      121 aatcatttcg caaactggtc tttatggggc tgagccgttt gtaatcctga cgatataagg
      181 taaatgtaaa tgcagaaatt tatctctctt gctcaaatcg acgagatcgt taatgtcata
      241 aaaaagaaga tttccattca gccagtgatc gggatgattc tcggctcagg gcttaacggg
      301 ctggctgatt ctgtccagaa tccggttcac attccataca gtgacctacc aaacttcccc
      361 acctcaaccg tccacggaca tgttggtcgc tttgtgatcg gtgaactaga aggcaagcct
      421 gttatcgtta tgcaaggacg aattcattat tatgaaggct acaccatggg cgaagtaacg
      481 ttgcctgtgc gcgtcatgca tcgacttgga gttcatagtt tatttgtaac caatgcggct
      541 ggcggtgtga acgagaattt cgtaccaggt gatgtgatgt tgatcactga tcagttaaac
      601 ctgatgggga tgtctggttt gaaccccttg atgggaccca acctcgacga gattggaccg
      661 cgcttcccag atatgagcca gccttacgac cgtgaatatt gtgaccttgc tcgtaaagtg
      721 gcaaagcaaa gcaacctaac cttgcgtgaa ggggtctacg ctggtttgag cggaccttcc
      781 tttgaatcac cagcagacct tcgtttcttg cgccttgccg gtgcggatgc ggtaggaatg
      841 tccacagtac cagaagtgat tattgcgcgt catggcggaa tgcgcgtttt agggctttca
      901 ggcgtaagta acaaagctaa tttagatggg tccactatta caacccacga agaggtgatt
      961 gaagctgggc gtgtgatcac acctaagatc gaaaccatcg tacggggcgt tcttcgcgga
     1021 atataaacgc gaacttggca aggttgttag atggtagatc tctttaagtt cctcgctgaa
     1081 tacgaggtat tgatttatat tgtcttggcg attggagcat tattttcctt tcgctggctt
     1141 tttcgttcat ggcgtgaatg gcaggtggct gtctttagtt tggaacgcga gttttcagct
     1201 cggcgttttg ggcgttctgc tttggtctca gcgatgatcg tgattttttt ctgcgtagag
     1261 ttcttcatgg catcgttcat catcccaggt ctccccgctg atttctttgt tgctacaccg
     1321 actctggacc tcatttccac accaaccggt acgctttcgc ccgaactgat gacccaattt
     1381 gcaaatcagc cgcaagtggc gcaacccaat gctgccgggt gtacccctgg gcaaatcgag
     1441 ttgacattcc ctgaggctgg cactgaaatt aaaggcatca tcgaactgac aggtactgtc
     1501 gacataccca attttggatt ttataaatat gagatcgctc caagtggcag tgacacgtgg
     1561 gcgacgattg cggcaggacg taccgttgtg aaagcaagtt ctcttgggcg ttgggatacc
     1621 acagcgctca cccctggcga ctatcaattg agattggtcg ttatcgataa tgaaggtaat
     1681 tcgcttacac cttgtgtcgt gcctgtgcag gttataccaa atcaataaaa ggtaatttca
     1741 attcatgtct gagttacaaa ttcgcccaac tgttgctact gatctaaccc gtttgatggg
     1801 aattgaccat tccatgacca gcgaagcagt ttggcagttg gaagttcgtc gtagtacggg
     1861 gcaggtggca gttacatttc gtgaggttcg tctaccgcgt gaaattcagg tgccataccc
     1921 gcataatcat tttgccttgg cagatgattg ggtaaagcgc tccatgatgt atacagcgat
     1981 caaggatgcc gagccggtcg gatatattag cttgttggag cgggggactg cttcaaccgt
     2041 gtgggtgctg gatctggcaa ttgatgcgaa gcaacgtcgc cgcggcgtgg ctgcagcttt
     2101 gttggcgtct gctcaggcat gggcggagtc gcgatcgcat cgtcgcctga ttctcgaagt
     2161 gcagtcaaaa aatgtcccag cgatccggct ggcgcagaaa tctggatttg agttctgcgg
     2221 gtataatgac cagtattacc ttaacaagga cgtagcacta tactttgcga aggtattaaa
     2281 gtaaggcgag cggttttacc gcccgttgca cgatttttat tggaagggtt gcatgattaa
     2341 cggttggcca gaaacact
//
LOCUS       DAOQJE010000626         2355 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_33824_length_2355_cov_5.362609, whole genome shotgun sequence.
ACCESSION   DAOQJE010000626 DAOQJE010000000
VERSION     DAOQJE010000626.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2355)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2355)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2355
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33824_length_2355_cov_5.362609"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            186..1679
                     /locus_tag="PLT08_14385"
     CDS             186..1679
                     /locus_tag="PLT08_14385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE05711.1"
                     /translation="MMLEKKQNYLNYALWVVNIALWMTVGLYAFVGIYSRYLADDYCE
                     AMRVSDASSPLEATIQRYMTPGWPRETMRYSNILFVGISELFGRYSMQVSIPLLILLW
                     GIGVTWLLHEIFLFLKLKISLKEELFLSSLFVFISFLMSSNLFQVIYWRSSAMTHFAP
                     LVFGSFFCAFLLNVFRQDEKKSISIWVYIGSYFISFLLAGFSEPPVTVAITVCTFLMI
                     YFFFWDSSKNKNRRLILLTVTLAGVISGLAAMFLSPAVSDYQRGEPVDIVAVLLNSFL
                     YSFQFIVDLLKSFPLPLAVCLLLPALFFFVYSPTLQENTSPLNSRKLWSGILILFIVV
                     WILIAASFSPSVYGQGYPAGRARFLGSSMFVAFIIFLGASLGMIFFNSLKLLSLPLQK
                     IAMFLFVLIGVVYPLRRDVGIYSSDLPESQKQAMYWDLRYAQIQRKISQGETDLQIPA
                     FSGIHGIKELDLRTNFVYNVCAAKLYGANSISTYSVKEKDLMEALSE"
     gene            1672..>2355
                     /locus_tag="PLT08_14390"
     CDS             1672..>2355
                     /locus_tag="PLT08_14390"
                     /inference="COORDINATES: protein motif:HMM:NF012888.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase"
                     /protein_id="HNE05712.1"
                     /translation="MSDLTPILVTGSHRSGTTWVGRMLAADASTAYISEPLNVHHRPG
                     VYRTSVKYWYTYITDENGADYLPAFNELLDFKYHTVRETLALRSFKDFLRMGRDFHTF
                     FNGSMQGRRILIKDPFAVFSTPWFEKQLHCKVVITVRHPGGFVSSLKRLGWNFDFNNF
                     LNQPQFMRDHLEGDRAAMEAMPGDDLVGQAALLWKIIYRFVHGTRSQFPRFHIVRHED
                     LSRDPINGYQ"
BASE COUNT          572 a          497 c          512 g          774 t
ORIGIN      
        1 ccgaaaaaga tttccaagac caatggaatg aattgatgcc caagaaagat ttatttcttg
       61 tgaccgattt caaagacctc gaacttcaac ctttcctcaa agcgaagcta agtacctatc
      121 ccatcttcgc tgaagggaat gggtatgtga tctatcaatt gaagccctaa acaggatatt
      181 gtttgatgat gcttgaaaaa aaacagaatt acttgaatta cgcactttgg gtggtaaata
      241 ttgctttatg gatgaccgtt gggctttatg cttttgtcgg aatttattcc cgctaccttg
      301 cagatgacta ttgcgaggca atgcgggttt cagatgcttc ttctcctctt gaagccacca
      361 ttcaacgtta tatgaccccg ggctggccgc gcgaaacaat gaggtattcc aatatcttat
      421 tcgtggggat aagtgaactg ttcggtaggt acagtatgca ggttagcatc cccttgctga
      481 ttttgttgtg gggtatagga gtaacttggt tacttcatga aatctttctt tttttgaaac
      541 taaagattag cctcaaagaa gaattattct taagctcgtt gtttgtattt attagctttc
      601 taatgtcttc gaacctgttt caggttattt attggcgttc atctgccatg acacactttg
      661 cgcctctggt atttggctct ttcttttgtg catttttgtt gaatgttttc cggcaggatg
      721 aaaagaaatc catctctatt tgggtatata ttggctcgta ttttatttct tttcttctcg
      781 ctgggttttc agagccgccg gtaacggttg ccattaccgt atgcacattt ttgatgattt
      841 actttttctt ttgggattct tcaaaaaata aaaatagacg tttgatcttg ttaactgtca
      901 cgcttgctgg ggtgatttca gggttggctg ccatgttctt atcccctgcc gtgagtgatt
      961 atcagcgtgg cgaaccggtg gatattgttg cagttctact taactcgttt ttatacagct
     1021 tccagtttat tgtggatctg ttgaagagct ttcctttgcc tttagctgta tgtttacttt
     1081 tgcctgcatt atttttcttc gtttattctc caaccctcca agaaaatacg tctccattaa
     1141 attcccgtaa gttgtggtcg ggcatattga ttttgtttat tgtcgtttgg atattaatcg
     1201 cagcaagttt ttctccgagc gtctatgggc agggataccc tgctgggcgg gcaagatttt
     1261 tgggcagcag tatgtttgtg gcatttatta tatttttggg cgcctcgtta ggcatgatat
     1321 ttttcaattc tcttaaattg ctttctttgc ccctacagaa aatagccatg tttttatttg
     1381 tattgattgg cgtggtttac ccgcttcgaa gagatgttgg aatatattct tcagacttgc
     1441 cagagagcca aaaacaagcc atgtattggg acttacgata cgctcaaatt cagcgtaaga
     1501 tctctcaagg agaaacagat ttacaaatcc cagctttttc aggtatacat ggcataaaag
     1561 aattagattt acgcacaaac tttgtttaca atgtttgtgc tgccaaatta tatggcgcga
     1621 attcgattag tacatatagc gtgaaagaaa aagaccttat ggaggcacta agtgagtgac
     1681 ctaaccccta ttctcgtcac tggctcgcat cgtagcggaa ccacttgggt tggtagaatg
     1741 cttgctgccg atgcaagtac tgcatatatc agcgaaccgc ttaatgtaca tcaccgtccc
     1801 ggtgtatatc gcacgtcggt caaatattgg tatacctaca tcaccgatga gaacggcgca
     1861 gactatctac ctgctttcaa cgagcttctt gattttaagt atcatacggt gcgtgaaacc
     1921 ctcgcactgc gttcattcaa agactttctg cgcatgggac gggacttcca tacatttttc
     1981 aatggcagca tgcaaggacg gcgaattctg atcaaagatc cttttgctgt attttctaca
     2041 ccgtggtttg agaagcagtt acattgcaaa gtggtcatca cagttcggca ccccggtggt
     2101 tttgtcagca gcctcaaacg ccttggctgg aatttcgatt tcaacaattt tctcaatcag
     2161 ccgcaattta tgcgggatca tttggaaggc gaccgcgccg ccatggaagc catgccaggc
     2221 gatgaccttg ttgggcaagc tgcattgctg tggaagatca tttaccgctt cgtacatggg
     2281 acccgcagcc agttcccccg atttcacatt gtgcgccacg aagacctttc ccgcgacccc
     2341 atcaacgggt atcaa
//
LOCUS       DAOQJE010000627         2352 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_33892_length_2352_cov_5.302133, whole genome shotgun sequence.
ACCESSION   DAOQJE010000627 DAOQJE010000000
VERSION     DAOQJE010000627.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2352)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2352)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2352
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33892_length_2352_cov_5.302133"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..611)
                     /locus_tag="PLT08_14395"
     CDS             complement(<1..611)
                     /locus_tag="PLT08_14395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE05713.1"
                     /translation="MSADKSNITRASELTAANLRSVAKTISAQQLSRLTLPEIEAVVQ
                     LVSKIMPAGNVPGMILSGLARLPGRRIPVQKLQQDVTALFSGVEQILDSAVYGAFFAG
                     PAAVLWGYQNLLKLAGKDPESAFPEGMWQFYVDYALREDTARHMNETHGFDTILAQHN
                     IKLDKVDRLTAWFMAAVACLHQYPALLENEWRERVAISLLEKT"
     gene            651..1523
                     /locus_tag="PLT08_14400"
     CDS             651..1523
                     /locus_tag="PLT08_14400"
                     /inference="COORDINATES: protein motif:HMM:NF013247.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class IV"
                     /protein_id="HNE05714.1"
                     /translation="MSRPNLFANLLGSGYIIQLMLHGYRLTPHGRIALNFNASSLDEL
                     TGRLSDGFYTTFSTLAQGTKVLGLKAHFDRLYQPAKELGLHPSADEQTVRKEIVDLAK
                     ALLPQESRIRLILTKDTGDVYLGIQALIPPSKAVYENGVHVITAEMARHDPRIKGTDF
                     ITQSAEQRKLVKDDVYEILLTKNGKVLEGMTSNFYAIKGRTIITARNGILLGVTRHAV
                     LKLARGEGMSIQYRSPLVGEKFDEAFLTSSSRGVVPIVQIDGTSVGQGKVGAWTKRLS
                     LAYQAYVETRSESI"
     gene            1618..>2352
                     /locus_tag="PLT08_14405"
     CDS             1618..>2352
                     /locus_tag="PLT08_14405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE05715.1"
                     /translation="MQKKILSLLLFMWVLSACGSSASEPQVITASGDITAAVEQYRAL
                     LGENNGGDPGTKGSSGYREINWDSLPDEESAPNLYAPDFFNQPEAPRARGIVLNTAGD
                     GLMVSASAANANGTLPRFGNINPQYADIFKTFSDEKLFSPVGSNIVEATFFVPGTNTP
                     AAVRGFGGIYADVDVDYYGFEFFDAAGTSLGKYQIPLADNGLAFVGVAFDEPVIARVR
                     IQFGTGVIGPDDGAGDVDISVMDNFIF"
BASE COUNT          617 a          539 c          592 g          604 t
ORIGIN      
        1 aaagtttttt cgagtaaaga aatcgccact cgttcacgcc attcattttc aagcaacgcc
       61 gggtattgat gcaaacaagc aactgcagcc atgaaccaag ctgtaagccg gtccactttg
      121 tctaatttga tgttatgttg tgcaaggatc gtatcaaagc catgcgtctc gttcatatga
      181 cgggctgtat cttcacgcag agcatagtct acataaaatt gccacatccc ttcggggaac
      241 gcagactcag ggtctttgcc ggctaacttc aacagatttt ggtaccccca taaaacagca
      301 gcgggtccgg caaagaacgc gccatacaca gcactatcca agatttgttc tacaccgcta
      361 aaaagagcag ttacatcttg ttgtaatttt tggacgggga tacgtcgacc gggcaaacgt
      421 gccagcccgc taaggatcat accgggcaca ttccctgccg gcataatctt ggagaccaat
      481 tgcaccaccg cctcgatctc tggtaatgtg aggcgtgaaa gctgttgcgc agaaatggtc
      541 tttgcaaccg agcgcaaatt agcagcagtg agctctgaag cacgtgtaat gttggatttg
      601 tctgcgctca tctatgaaat tatatttcac cttcatcttg atggattttt ttgtctcgtc
      661 ccaatctgtt tgcaaatttg ttaggtagcg gatatataat tcagctcatg ttgcatggat
      721 atcgacttac accacatggg cgtattgccc ttaatttcaa cgcctcttct ctcgacgagt
      781 taacgggtcg gctttcagat ggcttttaca ccacctttag caccctcgcg caagggacaa
      841 aggtgcttgg cttgaaagct cactttgata ggttatatca acctgcgaaa gaactagggc
      901 tgcatccctc agcagacgaa caaaccgtcc gcaaagagat cgtggacttg gcaaaagcgc
      961 tcttgccaca agagtcccgc attcggctta tcctcacaaa agacacaggt gacgtttacc
     1021 ttggcattca agcattgatt ccgccttcta aggcggtgta tgaaaatggt gtacatgtta
     1081 tcacagcaga aatggctcgc catgatccac gtataaaggg aacggacttc atcacccaaa
     1141 gcgcagaaca acgcaaattg gtaaaagatg acgtctatga aattttgcta accaaaaatg
     1201 gaaaagtctt ggaaggcatg acttccaatt tttacgcaat caaaggcagg acgatcatca
     1261 cagcccgaaa cggaatctta ctgggggtga cacgccatgc cgtgctaaag cttgcgcgag
     1321 gggaggggat gtctattcag tatcggtctc cgcttgtggg tgaaaaattt gacgaagcct
     1381 ttcttacatc cagttcgcga ggcgtggtgc cgatcgtgca aattgatggg acgtcagtgg
     1441 ggcaggggaa ggtcggggcg tggacaaaac gtctctcttt agcgtatcag gcttacgtcg
     1501 aaacgagaag cgaaagcatc taaagttaga acctgtatct cactaaagtc ggtgggatag
     1561 atagcgcgac atttatataa tcgcgctatc tatttttatt ggacaaagga gttcgagatg
     1621 cagaaaaaaa tactgagttt gttattgttt atgtgggtgc tctccgcttg cggaagcagc
     1681 gcctcggagc cgcaagtgat taccgcatct ggcgatatta ccgcggctgt agagcaatat
     1741 cgtgcattgc taggtgagaa caacggcggt gaccctggca cgaagggatc ttcgggctat
     1801 cgcgaaatta attgggacag cctgccagat gaagaatcag cgcctaatct atatgcacct
     1861 gattttttca atcaacctga agcgccgcga gctcgtggca ttgtcttgaa tacagcaggg
     1921 gatgggctca tggtgagtgc cagtgcggca aatgcgaatg gaacgttgcc acgctttgga
     1981 aatatcaatc cgcaatatgc cgacatcttc aagacattca gcgatgaaaa attattttcg
     2041 ccggtcggca gcaacattgt agaagccacg ttctttgtgc ctggcaccaa cactccagcc
     2101 gccgtgcgcg gatttggcgg catctatgcc gatgtggacg tggattatta tggctttgaa
     2161 ttttttgatg cagctggaac ttcgttaggg aagtatcaaa ttccactggc agacaacggc
     2221 ttagcatttg ttggcgttgc gttcgatgaa cctgtgatcg cacgagtgcg cattcaattt
     2281 ggcaccggtg tgatcggacc cgatgatggg gcaggggatg tagatatatc tgtgatggat
     2341 aactttattt tc
//
LOCUS       DAOQJE010000628         2350 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_33953_length_2350_cov_5.680610, whole genome shotgun sequence.
ACCESSION   DAOQJE010000628 DAOQJE010000000
VERSION     DAOQJE010000628.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2350)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2350)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2350
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33953_length_2350_cov_5.680610"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..403)
                     /locus_tag="PLT08_14410"
     CDS             complement(<1..403)
                     /locus_tag="PLT08_14410"
                     /inference="COORDINATES: protein motif:HMM:NF024937.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4126 domain-containing protein"
                     /protein_id="HNE05716.1"
                     /translation="MELLLGIFTAFGLSASAGLNAYIPLLVVGVIAHYAPNTLNLASP
                     FDLLANPWILILLGVLVIIEMVADKVPAVNHINDLIQTIIRPVAGAIAFAASANVITD
                     VNPVLALACGLLVAGSVHTVKAAAVRPAVTAT"
     gene            complement(463..1311)
                     /locus_tag="PLT08_14415"
     CDS             complement(463..1311)
                     /locus_tag="PLT08_14415"
                     /inference="COORDINATES: protein motif:HMM:NF021384.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2085 domain-containing protein"
                     /protein_id="HNE05717.1"
                     /translation="MQTNPQPSKLETIASWVVPIAAIAAVIAWFIIAPEGALGKLDAI
                     GYAVCHRIDVRSFHIGDRQLPLCARCTGEFYAAGLTLLFQMVVSGKRSKLPSRGVMAV
                     LFGFFVAFGVDGTNSYLYLLKETGRNSGFLAQIPNLYVPNNVLRLFTGSGMGIALAAM
                     LYPVINQTLWRTLDDRPAIEWKQLGILVAGIIGINLLVLTDSPFVLYPIAYISALGTL
                     SLLVIVFGLLWLIIMKQDNSFEHPRQLWLAFASGLTLALLLILSIDLMRLNFTGTWSG
                     FPGLSG"
     gene            1402..>2350
                     /locus_tag="PLT08_14420"
     CDS             1402..>2350
                     /locus_tag="PLT08_14420"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: protein motif:HMM:NF002872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006002 - fructose 6-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent 6-phosphofructokinase"
                     /protein_id="HNE05718.1"
                     /translation="MKRIGILTGGGDAPGLNAVIRAAVKTAINEYQCEVIGIRDGYDG
                     FLDTQNGIVPLGMEEVRGILPRGGTILGAANRGNPYARKVIRDGKEVTIDVSDEIVKG
                     IKELGLDALLVLGGDGTLHIAHELAQKGVPVIGVPKTIDNDIGGTEITFGFDTALNIA
                     TEALDRLHTTAESHHRVMVVELMGRDAGFITLNAGISGGADVILIPEIPFKFENIMKK
                     IQSRSGQGRKFSLIAVSEGAKPIGGMQVYSRKGDEVYVPRLGGIGQVVAEFIEQQGFE
                     SRVTVLGHVQRGGTPTAFDRGLATRYGAAAVRLAASGRFD"
BASE COUNT          588 a          509 c          717 g          536 t
ORIGIN      
        1 ttgtggctgt gaccgctggg cgcactgctg ctgcctttac ggtgtgaaca ctgccagcta
       61 ccagcaaacc gcaggcaagt gctagcacgg ggttgacatc ggtgatgaca ttggcactgg
      121 cggcgaaggc gatggcacct gcgaccgggc ggatgatggt ttggatcagg tcgttgatgt
      181 gattgaccgc gggtacttta tctgccacca tttcaatgat gaccaacacg ccgagtagga
      241 tgaggatcca cgggttggcg agcaggtcga aaggggaggc gaggttgagg gtgttgggag
      301 cgtaatgagc gatgacaccc accaccaaaa gtggaatgta ggcattcaac ccggcgctgg
      361 ctgaaagacc aaaggcagta aaaattccaa gtaaaagttc catttcttct ccttatcaaa
      421 gatcgtacaa cttgattata cgatttttga gtatattcgt tgttagcctg atagaccggg
      481 gaacccgctc catgtgcctg tgaagttgag gcgcattagg tcaatgctca agatgagcaa
      541 caatgccagg gtcaagccgg aggcgaacgc cagccaaagc tgacgtggat gttcgaagga
      601 gttgtcttgc ttcatgatga tgagccatag caatccgaag acgatcacca acagcgaaag
      661 agtgcccaag gcgctgatat aagcaatggg gtacaacacg aagggactat cggtaagaac
      721 aagtaagttg atgccgatga tgcccgctac cagaatcccg agttgtttcc attcgatggc
      781 ggggcggtca tccaaggtgc gccagagggt ttgattgata accgggtaga gcatggcagc
      841 gagggcgatg cccatgccgc tgcccgtaaa gaggcgcaag acattattgg gcacatatag
      901 atttggaatt tgcgccagaa aaccagagtt tctgccggtt tcttttaaca agtacaggta
      961 agaattggtg ccatccacgc caaaggcgac gaagaaacca aagagcaccg ccatgactcc
     1021 gcgcgaaggg agcttgctgc gttttccact tacgaccatt tgaaaaagca gggtaagccc
     1081 ggcggcatag aattcaccgg tacagcgggc acataggggg agttgacggt cgccaatgtg
     1141 gaaggaacgt acgtcaatgc gatgacagac agcatagcca atggcatcga gcttacccaa
     1201 ggcgccttca ggcgcgataa taaaccaggc gattacggct gcaattgcgg caatgggaac
     1261 gacccaactg gcaatggttt caagttttga gggttgtgga tttgtttgca ttttgagatt
     1321 atacccagcc tgatgtgaaa atctctcttt tgaggaatat ttaatatttt gcgttatcat
     1381 cttccatata gaggtgacat catgaaacga attggaatcc ttacaggcgg cggcgatgcg
     1441 ccggggttga atgcggtcat ccgtgcggcg gtaaagacgg cgatcaatga atatcagtgc
     1501 gaggtaatcg gcatccgtga tgggtatgac ggttttctcg atacacagaa tggaattgtg
     1561 cctttaggca tggaagaggt gcgcggaatc cttccgcgtg gtgggacgat tcttggggcg
     1621 gcgaatcgtg gtaacccgta tgctcgcaag gtaattcgag atggcaaaga agtcacgatt
     1681 gatgtgtcgg atgaaatcgt gaaaggcatt aaagaattgg gcttggacgc attgcttgtg
     1741 cttggtggag acggcacttt gcatattgca catgaactgg ctcaaaaagg agtgccggtg
     1801 atcggtgtgc ccaagacgat tgataacgac attggcggaa cagagatcac ctttgggttt
     1861 gatactgctt tgaatatcgc tactgaagca ttggatcggt tgcatactac ggcagagtcg
     1921 catcaccgcg tgatggtggt ggagttgatg gggcgcgatg ccgggtttat cacgttgaat
     1981 gcaggtattt caggcggggc agatgtgatt ttgattcctg agatcccatt caagtttgag
     2041 aacatcatga aaaagattca gtctcgcagt gggcagggac ggaagttcag cttgatcgcg
     2101 gtttctgaag gcgcgaagcc tatcggtggc atgcaagtct attcgcgcaa aggggatgag
     2161 gtgtatgtgc cgcgcttggg aggaatcggg caggtggttg ccgaattcat tgaacaacaa
     2221 ggcttcgaat cacgcgtgac cgtacttggg cacgtccaac gcggtgggac gcctaccgcc
     2281 ttcgaccgtg gtcttgcgac ccgttatggc gcggcggctg tccgcttggc ggcgagtggc
     2341 agattcgacc
//
LOCUS       DAOQJE010000629         2345 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_34095_length_2345_cov_4.449345, whole genome shotgun sequence.
ACCESSION   DAOQJE010000629 DAOQJE010000000
VERSION     DAOQJE010000629.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2345)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2345)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2345
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34095_length_2345_cov_4.449345"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..719
                     /locus_tag="PLT08_14425"
     CDS             <1..719
                     /locus_tag="PLT08_14425"
                     /inference="COORDINATES: protein motif:HMM:NF012382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HNE05719.1"
                     /translation="KGRKIAGIVITNPDNPTGMTIAVEKQVSLAKAALEAGVAFVLFD
                     WMYHYVTDESPMDLNSFLKAFTPEERKRIMFLDGITKSLGGSNIRNCHLIADEAVIKF
                     ITARASHGVIPSFFSLAVAMAAYEMGFNEAAKTIIEPTNASRVALKKLLNESGLQHII
                     GKGYYAFMNVGEFIKAKGWADTEPLGQYLAENHGVAVVPGAFFSPFGGEWIRFSYATP
                     VERTEGAFKRLMEGLNELKK"
     gene            805..>2345
                     /locus_tag="PLT08_14430"
     CDS             805..>2345
                     /locus_tag="PLT08_14430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE05720.1"
                     /translation="MPQQFAEKYLLALEESIKEKPKGGLAGFEQEWNLLDNDLRPLLT
                     VGAGPSQHSFVDYLLAECVPTWQKQFSQLEVFHWMVEWATRPYYSPRGAIYEARLMEA
                     SLMNALHRAGLSFGERLHYWHGNLLFLTDIGHQSIPGNWSLAKRRYLEKCVDLYGDTL
                     ATTGIHTNISLPDPLFAWDFMHLSASERGDKHLDDYKSEFYITATRLLRAFASLFIAT
                     SASTPMQAQVKDGRAAVILTEFDSIRNLTFPNPREVDLPDLYRSYNDYLQISYDLVRR
                     GVRFGNNNWTPVRARSFSEPVERIISTTSEQLESLYARGLFAAGESTPPDEMAKQVER
                     QNLMARINLPMGRVEIRVDEGGHSLDLDVANMTFRYLLMLKIYADPKFARGFRYDSED
                     ITRARTNENLAAKHGLRAEIENPLTGKPIVVRDFLKWTLNEIKPMAQALNMWNDLSPL
                     VEMAEGGKNTAEKIRARLKMEIGNSDEVPMDLLKEFLYEHEAQVKSDVERVCADYNAL
                     GSDAS"
BASE COUNT          582 a          621 c          546 g          596 t
ORIGIN      
        1 caaaaggacg caagatcgcg ggtattgtca tcaccaaccc tgataaccct accggcatga
       61 ccattgccgt tgaaaagcag gtctcgcttg ccaaggctgc cctcgaagcg ggagttgcct
      121 tcgtactctt cgattggatg tatcactacg tcaccgatga atcgccaatg gatttgaatt
      181 ctttccttaa ggcattcacc cccgaagagc gcaaacgcat catgttcctg gacggaatca
      241 caaaatcctt gggcggttca aacatccgca actgtcacct cattgcagat gaagccgtta
      301 tcaagttcat caccgctcgt gcttcccatg gtgtcatccc ttcgttcttc tcgcttgcgg
      361 ttgccatggc tgcctacgaa atgggtttca acgaagctgc caagaccatc attgaaccga
      421 ccaatgccag ccgagttgcc ctcaagaaac ttctcaacga aagcggactt caacacatta
      481 tcggtaaagg ctattatgcc tttatgaacg tgggcgaatt tatcaaagcc aaaggctggg
      541 cagatactga accgctgggt caataccttg ctgaaaatca cggcgttgcg gtcgttccgg
      601 gtgcattttt ctcacccttc ggcggcgaat ggatccgctt ctcgtatgcc acgcccgtcg
      661 agcgtaccga aggcgcattc aaacgcttga tggaaggctt gaatgaatta aagaagtagt
      721 gacagaaagt agaaagtgat gttcgttctc actttctact ttctgtttta tagccattat
      781 tccaatttat cttaggaaat atccatgcca caacaatttg ccgaaaaata tttacttgcc
      841 cttgaagaat ccattaaaga aaaaccaaaa ggcggtctcg ctgggtttga acaggaatgg
      901 aatctgcttg ataacgatct ccgtccgctg ttaactgtcg gtgccggacc cagccagcac
      961 tcctttgtgg attatctgct tgcagaatgc gtccctacat ggcagaagca attcagtcag
     1021 cttgaagtct tccactggat ggtggagtgg gcaacccgcc cgtattattc tccacgcggg
     1081 gcgatctatg aagcccgtct catggaagcc tcgctcatga atgctctgca tcgcgccggg
     1141 ctgagctttg gcgaacggct gcattattgg catggtaatt tgctcttcct taccgatatt
     1201 ggtcaccaat cgattcccgg caattggtct ttagccaagc gccgctatct cgaaaaatgc
     1261 gttgacctct atggtgatac cctcgccacc acaggcattc acaccaatat ctcgttgcca
     1321 gacccactct tcgcatggga tttcatgcat ctctctgcca gcgaacgcgg cgataaacat
     1381 ttggatgatt acaagtctga gttttatatt accgccacac gcctcttacg tgcttttgct
     1441 tctctcttca ttgcgacgag tgcttctacg ccgatgcagg ctcaggtcaa agacggacgc
     1501 gccgcagtca tcctcacaga atttgactca attcgtaatc tcaccttccc gaatccgcgc
     1561 gaagtggatt tgcctgacct ctaccgttct tataacgatt acttgcaaat ctcctatgac
     1621 cttgtccgcc gtggcgtgcg cttcggtaac aacaactgga cacccgtccg cgcccgcagt
     1681 ttctctgagc cggtagagcg cattatttcc acgacgagcg aacagcttga gtctctctac
     1741 gctcgcggac tcttcgccgc aggcgaatcc acgccgccag atgaaatggc aaaacaggtt
     1801 gaacgtcaga acttgatggc tcgtatcaac ctcccgatgg gacgtgtcga aatccgcgta
     1861 gacgaaggtg gtcatagcct tgaccttgat gttgccaaca tgactttccg ttatttgctc
     1921 atgttaaaaa tctatgccga cccgaaattt gcacgtggct tccgttacga cagtgaagat
     1981 attactcgtg cgcgcaccaa tgaaaatttg gcagccaaac atggcttgcg cgctgaaatt
     2041 gaaaatccac tcacgggcaa acctatcgtt gtacgagatt ttctcaagtg gacgttgaac
     2101 gagatcaaac ccatggcaca agccttgaat atgtggaatg acttgagtcc gcttgtggaa
     2161 atggctgaag gcggcaaaaa tactgcagaa aagattcgtg cccgcctcaa gatggaaatt
     2221 ggaaatagcg acgaagtgcc catggacttg ctcaaggaat ttttgtatga gcatgaggca
     2281 caagttaaat ccgatgtcga gcgtgtctgc gcagattaca acgccttggg tagtgatgcg
     2341 tccaa
//
LOCUS       DAOQJE010000630         2344 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_34127_length_2344_cov_4.509393, whole genome shotgun sequence.
ACCESSION   DAOQJE010000630 DAOQJE010000000
VERSION     DAOQJE010000630.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2344)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2344)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2344
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34127_length_2344_cov_4.509393"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            83..1855
                     /locus_tag="PLT08_14435"
     CDS             83..1855
                     /locus_tag="PLT08_14435"
                     /inference="COORDINATES: protein motif:HMM:NF015722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formylglycine-generating enzyme family protein"
                     /protein_id="HNE05721.1"
                     /translation="MKIVRNMILGLTLSLAVIFTSCGSPTPEIVTVVVTATSAPATEA
                     PAVVPTQPLAPVTLGGPQSGETIKWVDGSTLIYVPGGTFTMGYGGDAPVHSVSVDAYW
                     MQETKVTNEMYNLCVKAGACSSPTQELGGPVFTNPSFASNPVVGVTWDQAQAYCTWIQ
                     GSLPSEAQWEKAARGVNGNTYPWGEAAPTCDLLNYSNCYGRTTNVTTYEAGKSPFGVL
                     DIAGNVFEWVYDWYDANYYASSPADNPTGPQSGQYRSIRGSSFETAEEQVMSAIRRFN
                     EPTDSGRDIGFRCAVANPQPFAPYCQLTAHVPANQLDSSTTCQLPEVAVVNQYCQNGD
                     GYGVVQIPFNTTWEERGTRIQCVEQIEGGIRTLVCKGPRGIESTNEVMVCNPACTNQP
                     DVSSLNAFCPAGYTLDPSTGACAYTPILSQPTAGGCPLGYVTVQRGDQQVCAVGPAAD
                     NSCPIGLYFDDLAGMCVPPNGETNAPYGIDNTTLASQTYAGCTAGYSYNDSFQCCQPS
                     AGAAYPSCEPGFTFDSNSAACVPVLEEALNGTGCVAARVNTVKCVQLEDKVCAPIVSE
                     SRCVANLNCTWNEKDATCDPRVKP"
     gene            2088..>2344
                     /locus_tag="PLT08_14440"
     CDS             2088..>2344
                     /locus_tag="PLT08_14440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE05722.1"
                     /translation="MKKFLMIVSVLTVLTLALGACGPTATSTEAPQTEETQPSGQSAT
                     ADGSEQPTEAPTDVAIKTVDLVGPPMEVGSKYMFVDGTVLVA"
BASE COUNT          594 a          514 c          595 g          641 t
ORIGIN      
        1 atataaagag tttgtttaaa aacggtggtt gattttcttt tataataaat catctgctat
       61 ttatggcaga aaaggagttt gaatgaaaat cgttcgaaat atgatcctcg gcttgaccct
      121 ttcactggca gtgatattta cttcctgtgg atcgccaacc cctgagatag taacagttgt
      181 ggtcacagca acttcagcgc ctgcaaccga agcgccagca gttgttccaa cacagcccct
      241 tgccccggta acccttggtg gacctcaaag cggtgagacg atcaagtggg tcgacggcag
      301 cactctcatt tacgttccgg gcggaacctt tactatgggc tatggtggcg atgctcctgt
      361 acatagtgtg tctgttgatg cttattggat gcaggagacc aaagttacaa atgaaatgta
      421 taacctgtgt gtaaaggctg gagcatgttc ttcacccact caggaacttg gtggaccggt
      481 tttcaccaac cccagttttg caagtaaccc ggtggtgggt gtgacttggg accaagcaca
      541 ggcgtactgt acctggattc aaggcagctt gccttcagag gcacaatggg aaaaagctgc
      601 tcgtggtgtg aatggcaata cctacccttg gggtgaagct gcaccaacct gtgatctttt
      661 gaattactcg aactgttatg ggcggactac caatgttacg acctatgaag caggaaaaag
      721 cccatttggt gttttagata ttgcaggtaa tgtttttgag tgggtctacg attggtatga
      781 tgccaactat tacgcctcct cgcctgctga caaccctaca ggtccgcagt ctgggcagta
      841 ccgaagcatt cgtggcagct cgtttgagac cgcagaagaa caggttatgt ctgccattcg
      901 tcgctttaac gagccgaccg atagcggacg ggatattggt ttccgctgtg cggtggcgaa
      961 cccccagcct tttgcgccgt attgtcagtt aacagctcac gtcccagcta atcaattgga
     1021 ttcttccact acttgtcagt taccggaagt cgctgtggtg aaccaatatt gccagaatgg
     1081 ggatgggtat ggcgtagtgc aaatcccgtt caatacaact tgggaagaac gaggaacgcg
     1141 tattcaatgt gtagaacaaa ttgaaggtgg tatacgtacc ttagtttgta aaggtccgcg
     1201 tggtattgaa tcaactaatg aagtgatggt ttgtaacccc gcttgtacca atcagccaga
     1261 tgtgagcagt ttaaatgcat tctgcccggc tgggtatacg cttgatcctt ctacaggtgc
     1321 atgtgcttat accccgatcc tttcgcaacc tacggcaggc ggatgtccgc ttgggtatgt
     1381 aactgtccaa cgcggcgacc agcaagtctg tgcggtgggt cctgccgcag acaactcctg
     1441 cccgattggt ttgtattttg acgatttggc tggaatgtgt gtaccgccaa atggagagac
     1501 gaatgcacca tatggtattg ataacaccac tttggcttca cagacctatg caggttgtac
     1561 tgctgggtat agctacaacg atagcttcca gtgttgtcag ccttcggcag gggctgctta
     1621 cccaagttgc gaaccaggtt ttacctttga ttccaactct gcggcttgtg tacctgtttt
     1681 ggaagaagca ttgaatggaa ctggttgtgt ggctgcccgt gtcaacacgg tgaagtgtgt
     1741 acagcttgaa gataaggttt gtgctccaat tgtctctgaa tctcgttgtg tagcgaattt
     1801 gaattgtact tggaatgaaa aggacgctac ttgtgaccca agagtcaagc cttaatttag
     1861 aactctagaa ctatgaatca ataaacccat gaatacttca tgggtttatt gattcatagc
     1921 ccaaaatata tattgttacc cagtactata cccagtacta aatggtggag cgttgaataa
     1981 gacaaatggg tgatgctctt aagttccttt cgtcgaccat gatcattata ctgaagatat
     2041 gttttcatat tttgaaatca tgaattttta gaaaaggaga gtgaaacatg aagaaatttt
     2101 taatgattgt ttcggtccta actgtgttga ccctggcact tggggcatgt ggacctaccg
     2161 ccacgagcac cgaagcgcca cagactgaag aaactcagcc ttctgggcag agtgctacag
     2221 cagatggtag cgaacagccg actgaagcac ctacggatgt tgccatcaaa accgtagact
     2281 tggtaggacc gcccatggaa gtgggctcta aatacatgtt cgtggatgga accgtgctag
     2341 tggc
//
LOCUS       DAOQJE010000631         2341 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_34201_length_2341_cov_6.505249, whole genome shotgun sequence.
ACCESSION   DAOQJE010000631 DAOQJE010000000
VERSION     DAOQJE010000631.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2341)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2341)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2341
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34201_length_2341_cov_6.505249"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1178
                     /locus_tag="PLT08_14445"
     CDS             <1..1178
                     /locus_tag="PLT08_14445"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HNE05723.1"
                     /translation="APLYPTFLAFIYSLSGLGFSRFFAARCAQAILLGAPLAPLTYGI
                     AKRFFVDIDSNEKISRLAASIVACYPMLLVYPLGLGTENLFFILLLFSFLFLLRTLAA
                     PSKLNFLFAGVFLGLAALTRSVVLPFVGVSLFYLFYLHRTKVWITFVAFCLIISPWMI
                     RNSLLHHKLTGIETSMGYNLYLGYYPEGNGSFIFGPSLDLLPIMDDSERDAIGTEKAL
                     EFIRAEPGRFVPLALNRLGFFFGLENRVLMYFYSNNLLGYISTPILLLISAFFLLPFV
                     FVSILAVFGLVSQKPSQPRALLNLLLFAYIMPYVLILSEDRFHLALIPFLAMLASYTF
                     VLLKERRYVHYVSIRTLAFAGILMILLVLNWGLELHRDADKIAAILQPNGNQLYLPY"
     gene            1218..1832
                     /locus_tag="PLT08_14450"
     CDS             1218..1832
                     /locus_tag="PLT08_14450"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014566.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HNE05724.1"
                     /translation="MKYLTGEKLSFDWKVVTVTIVSTLLLMVDYYHKIFAYKYIDRIV
                     LYFFIPLLITLLIFRENPKEYGFSFGDWKLGLTYTLIGILVMAPVIYFLGHGNASMKS
                     YYERFLPGLPWTTFLDLFGWEFFFRGWILFTYSRKFGPEALWLHAVPFALGHLGKPEI
                     ETLSTIFGGFAFGWVAWRTKSFFYAFLIHWFIGTFIIIVAAGAI"
     gene            1834..2277
                     /locus_tag="PLT08_14455"
     CDS             1834..2277
                     /locus_tag="PLT08_14455"
                     /inference="COORDINATES: protein motif:HMM:NF025054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNE05725.1"
                     /translation="MEPYRIRPALESESTQIKDLIHEVGINPTGLDWRRFLVAVDDSG
                     RVIGCGQIKPHGGDILELASIAVNPERQGQGIARKIIEQLLEQNPRPLYLMCIEHNGP
                     MYEKFGFRALGYDESPRYFQRMQKLFDVAKVVRRVNESLLVMKLE"
BASE COUNT          532 a          488 c          509 g          812 t
ORIGIN      
        1 gagcgccact atatccgacc tttctagcgt tcatctactc ccttagcgga cttggctttt
       61 cgcgcttctt tgccgcccgt tgcgcccaag ccattctttt aggtgcgcct cttgcgccac
      121 tcacatatgg tattgcgaag cgtttctttg ttgatattga ttcaaacgaa aagatttctc
      181 gcctcgcagc atcgattgtt gcctgttacc ccatgctgct tgtttaccca cttgggttag
      241 gcaccgagaa tctttttttc atcctgcttc ttttttcttt cttgttcctt ctcagaactc
      301 ttgcagcccc atctaaactc aactttttgt ttgcaggcgt ttttctaggt ctagctgccc
      361 ttacacgttc tgtggttttg ccttttgttg gagtgagtct tttttatttg ttttatctcc
      421 atcgcactaa agtgtggatt acatttgtcg ctttttgctt aatcatctcc ccttggatga
      481 ttcgtaactc tttgcttcat cataaattga ctgggatcga aacctccatg ggatataacc
      541 tttatcttgg ctattatcct gaaggaaatg gctcctttat ttttggacca tcccttgatc
      601 tacttcctat tatggacgat tctgaacggg atgcaattgg tacggagaaa gcacttgaat
      661 tcatccgtgc agaacctggt cgttttgttc cactggcgct gaatcgcctt gggttctttt
      721 ttggcttgga aaaccgcgta ttgatgtatt tttactcaaa caatctctta gggtatattt
      781 ccaccccgat attgcttttg atttcggcat ttttcctttt gccttttgta ttcgtaagta
      841 ttcttgcagt ttttgggctt gtttcacaaa aaccttcaca accaagggct ttgctcaatt
      901 tacttttgtt cgcttatatc atgccttatg ttttgatctt atccgaagac cgattccact
      961 tggcgttgat tccgttcttg gcaatgctgg catcgtatac ttttgtgctt ttgaaagaaa
     1021 gaagatatgt tcactatgta tctattagaa cactggcatt tgcaggcata ttgatgatcc
     1081 tcttggtgct aaattggggg cttgaattac accgcgatgc ggataaaatt gccgccatcc
     1141 ttcagccaaa tggcaatcaa ttgtatttgc catattaatc tcacaggctg gggcgatttt
     1201 atactcagag gtaacgcatg aaatatttga caggcgaaaa gctaagtttt gactggaagg
     1261 tcgttacagt gaccattgtc agcaccttat tgttgatggt ggactattac cataaaatat
     1321 ttgcatataa atacattgac cgtattgttt tatatttttt tatcccactt ctaataacct
     1381 tacttatctt tcgagagaat ccaaaagaat atggtttttc attcggcgat tggaaactgg
     1441 gactcacata taccttaatt ggcatcttgg ttatggcacc agtgatctac tttttaggtc
     1501 atggcaacgc ttcaatgaag agttattatg aacgctttct acccgggctg ccatggacga
     1561 catttttaga cctatttggc tgggagtttt tcttccgcgg ttggatcttg tttacatatt
     1621 ctcgtaaatt cggaccagaa gctctatggt tacatgccgt tccatttgcg ctgggacacc
     1681 ttggcaagcc agaaattgaa accctgtcca ctatttttgg cggatttgct tttggctggg
     1741 tggcgtggcg cacaaaatca tttttctacg cattcttaat tcattggttc atcggaacat
     1801 ttattattat cgttgcggcg ggagcgattt gatatggaac cctatcgcat tcgcccggct
     1861 ttggaatcag aatcaactca aattaaggat ttgattcatg aagtgggaat caaccccacg
     1921 ggtttggatt ggaggcgttt tcttgtcgca gtggatgatt ctggacgagt gatcggttgc
     1981 gggcagatca agccacatgg aggcgacatc ctcgaattgg cttcgattgc agtgaaccct
     2041 gagcgccaag ggcagggtat tgcgcgaaaa atcattgagc agttgctgga gcaaaacccg
     2101 cgcccactgt atttgatgtg tattgaacat aatggaccta tgtatgaaaa atttgggttt
     2161 cgtgctcttg gttatgatga atctccgcga tattttcaac ggatgcaaaa gttatttgat
     2221 gttgccaaag tagttcggcg tgtgaatgaa agtttactag taatgaagtt ggagtagaat
     2281 cgcggcatga aaaatgtgct ttatacgttg gtgggtgtca tggctggttt tgcattagca
     2341 g
//
LOCUS       DAOQJE010000632         2341 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_34202_length_2341_cov_6.112861, whole genome shotgun sequence.
ACCESSION   DAOQJE010000632 DAOQJE010000000
VERSION     DAOQJE010000632.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2341)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2341)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2341
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34202_length_2341_cov_6.112861"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..206)
                     /locus_tag="PLT08_14460"
     CDS             complement(<1..206)
                     /locus_tag="PLT08_14460"
                     /inference="COORDINATES: protein motif:HMM:NF013412.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HNE05726.1"
                     /translation="MHIPQSLIDLVESGAHAHLVTINKDGTPHVTVVWIGVENDEIVC
                     AHLPRNQKVRNIERNGNVILSLEA"
     gene            complement(235..1257)
                     /locus_tag="PLT08_14465"
     CDS             complement(235..1257)
                     /locus_tag="PLT08_14465"
                     /inference="COORDINATES: protein motif:HMM:NF012546.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HNE05727.1"
                     /translation="MRSKTKFGYFLFFLIIFSAVLYALLPPTLPKDIPSDSKIDVQIG
                     PEWPAEVVAIKYLSSADNTLQPAIFYRPNITDRVPLLVMLHTWTGDYLQTLNAPLAQW
                     CIENNWAFIHPNFRGPNNKFEAIGSDLVIEDILSAVKFAETYSNIDTHNIYLVGASGG
                     GHAALMVAATHPNVWAGVSVWVPITDLSVWYEETKTLDGQYRIFLEQALGGAPGTSSA
                     IDLEYKKRSPLTYLSPSIHLNLDINAGIHDGHNGPVPISHSLLAYSALVTAEDQLSAK
                     DIKYFVEQEKVPQHLIDPSLSDSSYGEKRPIFRAQSGTTRLTIFEGGHELVIDAVINW
                     LTQSLK"
     gene            1461..1835
                     /locus_tag="PLT08_14470"
     CDS             1461..1835
                     /locus_tag="PLT08_14470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE05728.1"
                     /translation="MDFFLPEDNLTRAVPEETHITTLAAQPYPDGRRLRVNLEITPFQ
                     KRPYIEITLTDANGQEIATTSIVEPLSWKLEFTMHIRGELQNPYTLNAHLYYPENGPS
                     DQPRQITFDVTPPPPSPEPDTN"
     gene            1848..>2341
                     /locus_tag="PLT08_14475"
     CDS             1848..>2341
                     /locus_tag="PLT08_14475"
                     /inference="COORDINATES: protein motif:HMM:NF013123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DPP IV N-terminal domain-containing protein"
                     /protein_id="HNE05729.1"
                     /translation="MLWHSSSSHNVTPNGDGSLRQSNRIFPLFKLTSLNKSFLLATFS
                     LLLFACTQEIEPTLPPEALNVPTDTPVPTPVDPASETAFLSIEENGYAHLFLFRSQTS
                     QLTRITLGAWNDIAPALNPKRTYIAFASDRNGNWDLYTLELKTGNIKQVTNTPQYDSA
                     PSWSP"
BASE COUNT          662 a          593 c          436 g          650 t
ORIGIN      
        1 tccgcttcca atgagaggat cacattccca ttccgctcaa tgttacgcac tttctgattg
       61 cgcggcaaat gagcacaaac gatctcatca ttttcgacgc cgatccatac cacggtgaca
      121 tgtggtgtgc cgtctttgtt gattgttacc agatgtgcat gggcaccaga ttcgaccaag
      181 tctatgagag attgagggat atgcatgggg tggctcctga gtatagaaaa atacttattt
      241 aagactttgt gttaaccagt ttatcactgc atctattacg agttcatgcc caccttcaaa
      301 tatagtgaga cgagttgttc cagattgtgc tctaaagatc gggcgctttt ctccatatga
      361 ggagtctgac aatgaagggt ctattagatg ttgcggcact ttttcttgct caacaaaata
      421 ttttatgtct ttagcagata actgatcctc tgctgtaact agagcgctgt atgctagtag
      481 tgagtggcta atgggcactg gtccattatg tccatcgtga attcctgcat taatgtctag
      541 gtttaagtgg attgatggag ataaatatgt gagaggagag cgtttcttgt actctaaatc
      601 tattgcagat gacgtgccgg gggcgcctcc gagagcctgc tctaaaaata ttctgtattg
      661 tccatctaat gttttagttt cttcatacca tacggatagg tctgtaatcg gaacccatac
      721 tgaaacacct gcccaaacat tggggtgggt ggcagccacc ataagggctg catgcccgcc
      781 tcctgatgct cctactaaat aaatattatg agtatcaata tttgaatacg tttctgcaaa
      841 cttaactgca cttaaaatgt cttcaataac caaatctgaa ccaatggctt caaatttatt
      901 gtttggacct cggaaatttg gatgaatgaa agcccaatta ttctcaatac accattgtgc
      961 aagaggagca tttaatgttt ggaggtaatc acccgtccat gtatgtaaca taacaagtaa
     1021 aggaacacga tcggttatat ttggtctata aaaaatagca ggttgaagtg tgttatctgc
     1081 agaacttaga tatttgattg caactacttc tgcgggccat tcaggtccaa tttgtacatc
     1141 gatttttgag tcagatggga tatctttagg aagagtagga gggagtaaag catatagaac
     1201 agcactaaaa ataatcaaga aaaacaaaaa atacccaaac ttagttttgg agcgcatata
     1261 tttatagaaa ttctttcggt atcaagattt attaatttct taagtgctag tatatactca
     1321 atttaatcat taggattgaa aatggaagag gtttttacaa acctatggct actgcctgtc
     1381 agtagagcct caaaccaccc taatgaaact gtaatccccc ttcctaatct cgtccaacct
     1441 caacgggtat aattctccct atggactttt tccttcccga agacaaccta acccgtgcag
     1501 tacctgaaga gactcacatc acaaccctgg cagcccaacc ctatccagat ggacgccgcc
     1561 ttcgcgtcaa cctcgagatc actcctttcc aaaaacgacc ctatattgaa atcactctca
     1621 ccgatgccaa tgggcaagaa attgccacta ccagcatcgt tgaacccctt agctggaaac
     1681 tcgaatttac catgcacatc cgtggcgagc ttcaaaatcc gtatacgctc aacgcccacc
     1741 tctactaccc agaaaacgga ccttccgacc agccccgtca aatcacattt gatgtaaccc
     1801 cgcctccacc ttcccccgaa ccggatacaa actaaacaat cttatccatg ctctggcatt
     1861 cttcttcctc acataatgtc accccgaacg gggatgggtc tcttaggcaa tcaaacagaa
     1921 tcttccctct gtttaaatta acatccctca ataaatcctt tctccttgcc acttttagtc
     1981 tccttctctt tgcctgtact caagaaatag aacccacgct gccgcctgaa gccctcaacg
     2041 tcccaactga tacgcccgtt ccaacgcctg tagacccggc tagtgaaacc gcattccttt
     2101 ctattgaaga gaatgggtat gctcacctct tcctttttcg cagccaaaca tcccagctca
     2161 cccgcatcac tctcggcgcc tggaacgaca ttgctcccgc ccttaacccc aaacgtactt
     2221 acatcgcctt tgcttcagac cgcaatggca actgggatct gtacaccctt gaactaaaaa
     2281 ccggaaacat taaacaagtc accaacaccc ctcaatacga ctcagctcca tcttggtcac
     2341 c
//
LOCUS       DAOQJE010000633         2340 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_34235_length_2340_cov_7.620131, whole genome shotgun sequence.
ACCESSION   DAOQJE010000633 DAOQJE010000000
VERSION     DAOQJE010000633.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2340)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2340)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2340
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34235_length_2340_cov_7.620131"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1439)
                     /locus_tag="PLT08_14480"
     CDS             complement(<1..1439)
                     /locus_tag="PLT08_14480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE05730.1"
                     /translation="MDCSIDDVIPLPISAADSFTIVLMKKLTDRLNNIQFTPRSTVWF
                     LLLIAALTYTLFFWQRGFYWDEFPWAWIYYRLGPAMLTKTFTTSRPFWGMIYQLTMPV
                     IGPNPWIWQLLAIVLRWVTAVLLWKILRALYPEHPKPALWASLFFLVFPGMGQQFIAF
                     MYSHFYIVLSALLLSMYLAIRAIQDEKRRWLYFSISYVLAFVNFITMEYFYFIEFGLL
                     IVFYQLLPGTFKERAKKTLLYFLPYLAIFIGITVWRTFFFEFQNASYQFVLLDKLKAD
                     IFSGLLYFLNQILVSFWRTVFEAWILPFLTIGTTGFGPLTLSLMLVLLFSSILLTGVY
                     LFAFKAESSNGLDFAKRAGLVGLAFWGLSGGSVWMIGNLPQFNFSLDRFMLPFMLGSS
                     ILLASLIALIKSPRWQMILVALLIGFAGSRHFRLEEIYRSDWLTQQELFTQMTERIPG
                     LKKGTILISNDIPVTYYSDNSLTGPLNWI"
     gene            complement(1424..2335)
                     /gene="arcC"
                     /locus_tag="PLT08_14485"
     CDS             complement(1424..2335)
                     /gene="arcC"
                     /locus_tag="PLT08_14485"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008804 - carbamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HNE05731.1"
                     /translation="MIDDKKVTVESQYQAAKQTCVHIADMIEQGWDVAIGHGNGPQVG
                     YILRRSEIAAKTAGMHEVPLDVCGADSQGAIGYAIQQNLQNILYQRGIKKKVVTVITQ
                     TLVDKTDPAFSKPAKPIGSFMDKEEAERRQQEQGWSVVEDAGRGWRRVVASPLPKEIV
                     ELETVQTLLEKGIITITVGGGGIPVVDPGDGNYEGVAAVVDKDYASSLLARSIGANLF
                     VISTAVEKVAINFGKPEQKWLDQITLAEAKAYLAEGIHFAKGSMAPKIQAIIWYLEAM
                     PNGKALITNPENIGRALKGETGTWIVP"
BASE COUNT          707 a          560 c          514 g          559 t
ORIGIN      
        1 taaatccagt ttaatggtcc tgtgagtgaa ttatcagagt aatatgtcac aggaatgtca
       61 ttagaaatta agatagttcc ttttttcaac ccagggattc gttccgtcat ttgtgtaaac
      121 aattcctgtt gggtcaacca atcactacga tagatctctt ccaaccgaaa atgtctacta
      181 cctgcaaagc caattaacag cgccactaaa atcatctgcc agcgcggact tttgatcaac
      241 gcgatcaagc ttgccaacaa aatgctagag cctagcataa acggcaacat gaaacggtct
      301 agcgaaaaat tgaactgcgg caaattgcca atcatccaca cggagccgcc agataagccc
      361 caaaacgcca agccgaccaa gcctgctcgt ttggcaaaat ccaaaccgtt tgaagactct
      421 gctttgaacg cgaagagata cactccagtg aggagaatgc ttgagaataa cagaaccagc
      481 atcaaagaca gtgtgagcgg accaaagcct gtggtgccaa tggtgaggaa aggcaaaatc
      541 cacgcctcaa acacggtgcg ccaaaaactg accagaattt gatttaagaa ataaagcagt
      601 ccagagaaaa tatccgcttt tagtttatcg agcaaaacaa attgataact cgcattttga
      661 aattcgaaaa agaaagtgcg ccaaaccgtg atgccaataa agatggcaag ataagggaga
      721 aaataaagaa gcgtcttttt cgcacgttct ttgaacgtgc ccggcaagag ctggtaaaaa
      781 acaatcagca aaccaaattc aatgaagtaa aaatactcca tcgtaatgaa attgacgaaa
      841 gccaacacat acgatattga aaaataaagc cagcgtcttt tttcatcttg aatggcgcga
      901 attgcaagat acatggaaag caacaaagca gacagcacaa tataaaaatg gctgtacatg
      961 aatgcgatga actgctgccc catgccgggg aatacaagga agaacaaact tgcccacaaa
     1021 gcaggtttgg gatgttcagg ataaagtgcg cgtaaaattt tccaaagcag aacggctgtc
     1081 acccagcgca gaacaatggc aagcaattgc catatccacg ggttgggacc gataacaggc
     1141 atggtcagtt gatagatcat cccccaaaac ggacgactgg tggtaaacgt tttcgtgagc
     1201 atagcaggac ccagccgata gtagatccat gcccaaggga attcatccca ataaaaaccg
     1261 cgctgccaaa agaaaagagt atatgttagc gcggcaatta acagcaaaaa ccaaaccgta
     1321 ctgcgcggtg taaattggat gttgttcaaa cggtcagtca attttttcat tagaacaatt
     1381 gtaaacgaat ccgctgccga aattggcagc ggaattacat cgtctatgga acaatccaag
     1441 taccggtttc gcctttgagc gcgcgcccta tattttcggg gttggtgatt agagctttgc
     1501 cgttcggcat ggcttcgaga taccaaataa tcgcttggat ctttggcgcc atggagcctt
     1561 tggcaaaatg gattccctca gccaagtacg ctttggcttc ggctagtgtg atctggtcca
     1621 gccatttttg ctcgggttta ccaaagttga tggcaacttt ttccaccgcc gttgaaatga
     1681 caaatagatt cgcaccaata gagcgggcta acaaagaaga ggcgtaatcc ttgtcaacca
     1741 cagccgccac gccttcatag tttccatcgc cagggtcaac aaccggaata ccaccgccgc
     1801 ctactgtaat ggtgatgatt cctttttcca acagggtttg aacggtttcc aattccacga
     1861 tttcttttgg caaaggcgag gcgacgaccc gcctccaacc acgacctgca tcttccacca
     1921 cagaccagcc ttgttcctgc tgacggcgtt ccgcttcttc tttatccata aagctgccaa
     1981 tgggtttggc tggctttgaa aatgccgggt ctgttttatc taccaaggtt tgagtgatga
     2041 cggtaacaac ctttttcttg atgccacgtt ggtagagaat gttctgcaaa ttttgttgaa
     2101 tggcgtaccc gattgcgccc tggctatcag caccgcacac atccaaaggg acttcgtgca
     2161 tgccagccgt tttggcagca atttcagaac ggcgcaaaat gtaaccaact tgcggaccat
     2221 tgccatgccc aatcgccaca tcccagcctt gttcgatcat atcggcaatg tgcacacagg
     2281 tttgctttgc cgcctgatat tggctttcta ctgttacttt cttatcatca ataatgagcg
//
LOCUS       DAOQJE010000634         2330 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_34494_length_2330_cov_7.842198, whole genome shotgun sequence.
ACCESSION   DAOQJE010000634 DAOQJE010000000
VERSION     DAOQJE010000634.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2330)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2330)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2330
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34494_length_2330_cov_7.842198"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..336
                     /locus_tag="PLT08_14490"
     CDS             <1..336
                     /locus_tag="PLT08_14490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin B12-binding domain-containing protein"
                     /protein_id="HNE05732.1"
                     /translation="AKVIARALRDAGMEVIYTGLRQTPEMIAEAALQEDVDVVGLSVL
                     SGAHMALTPRVMELLRTNGQSHVKVFLGGIIPDEDLHRLKEMGVTGVYGPGANTEDII
                     HDIKTAVAG"
     gene            450..1454
                     /gene="meaB"
                     /locus_tag="PLT08_14495"
     CDS             450..1454
                     /gene="meaB"
                     /locus_tag="PLT08_14495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0015819 - lysine transport [Evidence IEA];
                     GO_process: GO:0015822 - ornithine transport [Evidence
                     IEA];
                     GO_process: GO:1903826 - L-arginine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl Co-A mutase-associated GTPase
                     MeaB"
                     /protein_id="HNE05733.1"
                     /translation="MTSSQAILNGDRLALARMLTQVENNSPEGQTVLMELFPHTGKAH
                     LIGVTGAPGTGKSSLVNQLALHYRKKEDKRVAIVAIDPSSPFTGGAVLGDRVRMRDLS
                     GDPNVFIRSMASRGSLGGLAQATANVVQVFDAAGFDVVIVETVGAGQSEVDIARLAHT
                     TLVVEAPGLGDDIQAIKAGILEIADILVVNKGDRPGVENTEKALKTMLELAHPTERVF
                     KHHGTSMRVSAPKTTANAKMWIPPIQRTISTEGIGIAELAEAIARHVEHLRLSGDWAT
                     RERTRLEVELEALIREGLMTKFREQLTAEAYEKVLESVVERNVSPWEAAKGLLKGYSK
                     "
     gene            1451..2005
                     /locus_tag="PLT08_14500"
     CDS             1451..2005
                     /locus_tag="PLT08_14500"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024812.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HNE05734.1"
                     /translation="MSIVYGKRIRLRAVEREDVQKFHEWINDPEVTLNLAMYLPMSMQ
                     DEMNWFEGLSKRDPHEKPFAIEIRKGKAWKLIGNCGVFGIEFTNRNGELGIMIGNKSE
                     WNKGYGAEAMSLLQRHCFETLNLNRVYLRVYEENVPAVRAYEKAGFVLEGRQRQAVYK
                     KGKYEDVLFMSVLRSEWDTRMKEK"
     gene            2011..>2330
                     /locus_tag="PLT08_14505"
     CDS             2011..>2330
                     /locus_tag="PLT08_14505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate pyrophosphokinase-like
                     domain-containing protein"
                     /protein_id="HNE05735.1"
                     /translation="MHHVHHDLKVYGGIKLFGGTGSPELSQKIAEYLDLPLSGREVIE
                     FPNENLFVKLNGSVRGQDVYVIQTTSSPVHRNLMELLIMIQTLKLDSAARITAVVPYL
                     CYGRS"
BASE COUNT          596 a          523 c          654 g          557 t
ORIGIN      
        1 gcgaaagtta tcgcccgtgc gcttcgcgat gccgggatgg aagtcatcta caccggcttg
       61 cgccaaaccc ctgaaatgat cgcagaagcg gctcttcagg aagatgtgga tgtagttgga
      121 ttgtctgtgc tctcaggtgc gcacatggcg ttgaccccgc gcgtgatgga attgctgcgc
      181 accaatggtc agtcgcacgt caaagtgttc cttgggggta tcatccctga tgaagacctg
      241 caccgtttga aagaaatggg tgtcaccgga gtatatggtc ccggtgcgaa caccgaagac
      301 attattcacg atataaaaac agcagtagca ggataaaagc atctcaacac agagccctct
      361 gctctgtgtt ttgttcttaa taacaaacag gggcgacctg tctatttaaa gacaaggtct
      421 tttctgcaag aatgaattac aggaaattaa tgacttcttc ccaagccatt ctcaacggtg
      481 atcgccttgc ccttgcgcga atgttgaccc aagtggaaaa caattcgcca gagggtcaaa
      541 ccgtgttaat ggaacttttt ccgcacacgg gcaaagcaca cctcataggt gtcacaggcg
      601 caccgggcac gggcaaatcg tcgctggtga atcaactggc gctgcactac cgcaaaaagg
      661 aagataaacg agttgcgatc gttgccattg atccgtccag tccgttcacg ggcggcgcag
      721 tgttgggaga ccgagttcgc atgcgcgacc tttctggcga cccgaatgtc ttcattcgct
      781 cgatggcttc gcgcggctcg ctaggcggat tagcacaggc aaccgcaaat gttgtgcagg
      841 tctttgacgc ggcggggttt gacgtggtca ttgtggaaac cgtgggcgcc gggcaaagtg
      901 aagtggatat tgcccgcctc gcccacacga cgttagttgt tgaagctccc gggcttgggg
      961 acgacatcca agccattaaa gccggtatcc ttgagatcgc agatattctc gtggtcaata
     1021 aaggcgatag acccggtgtg gagaataccg agaaagcttt gaagaccatg cttgaattgg
     1081 cgcacccaac cgaacgtgtt ttcaagcatc atggcacgtc catgcgagtc tctgcgccga
     1141 agacgactgc aaacgccaag atgtggattc cgcccattca acggacgatc tccactgaag
     1201 ggatcggtat tgcagaattg gctgaggcaa ttgctcggca cgtggagcac ctccgcttga
     1261 gtggcgattg ggcaactcgt gagcgaaccc ggctcgaggt cgagttagag gcgttgatcc
     1321 gcgagggatt gatgaccaag tttcgagagc agttaacggc tgaggcgtat gaaaaggttc
     1381 tcgagtcagt tgtcgaaaga aatgtttcgc cttgggaagc ggcaaaaggc ttgttgaaag
     1441 gatattccaa atgagcatcg tatatggaaa acgaattcgt ttgcgcgcgg tggaacgtga
     1501 agatgtgcaa aagtttcatg agtggatcaa tgacccagaa gtgacgctca acctggcaat
     1561 gtacctaccc atgtcgatgc aggatgaaat gaattggttc gagggcttgt cgaaacgtga
     1621 cccgcatgag aaaccttttg ccattgaaat tcgcaaaggc aaggcttgga agttgatcgg
     1681 gaactgcggt gtgtttggga ttgagtttac gaatcgcaat ggcgagcttg gaatcatgat
     1741 cggcaataaa tcggaatgga ataaaggcta cggcgccgaa gctatgagtt tgttacagcg
     1801 tcattgtttt gagaccttga acttaaaccg tgtctattta cgggtatacg aagaaaatgt
     1861 acctgccgtt cgtgcctatg aaaaagccgg ttttgtttta gaaggaagac agcgccaagc
     1921 tgtttataaa aaaggaaaat acgaggatgt tctcttcatg agcgtgttgc gctcggaatg
     1981 ggacacacga atgaaggaga agtaatccac atgcatcatg ttcatcacga tttgaaagtg
     2041 tacgggggga ttaagttatt cggtgggacg ggttcgcccg aattatcgca aaagatcgca
     2101 gagtacctcg accttccatt aagtggacgc gaggtaattg agttccccaa tgagaacttg
     2161 ttcgtcaaat tgaatggcag tgtgcgtggg caagatgtgt acgtcattca gacgacgtct
     2221 tctcctgttc atcgcaactt gatggaattg ctcatcatga tccagactct gaagctggat
     2281 tcagcagcgc gcattacagc ggttgtgcct tatctttgtt atgggcgttc
//
LOCUS       DAOQJE010000635         2329 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_34524_length_2329_cov_7.447669, whole genome shotgun sequence.
ACCESSION   DAOQJE010000635 DAOQJE010000000
VERSION     DAOQJE010000635.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2329)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2329)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2329
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34524_length_2329_cov_7.447669"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..581
                     /locus_tag="PLT08_14510"
     CDS             <1..581
                     /locus_tag="PLT08_14510"
                     /inference="COORDINATES: protein motif:HMM:NF015549.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HNE05736.1"
                     /translation="AQLMSSIVFAGSAQFITAQLFSEGAPAFVMILTIAVVNLRHMLY
                     SASLAPYLKDLSLRWKVLLSYLLTDEAYAPSILHYEKEGLAKYKYWFLFGAGLSLWIN
                     WQISTAIGVFLGAAIPENLSLDFALPVTFIAMIVPVMKKFPVIAAALSAGITALLAYH
                     LPYKLGLILAALVGITVGMLLENLQAAQKEAK"
     gene            578..898
                     /locus_tag="PLT08_14515"
     CDS             578..898
                     /locus_tag="PLT08_14515"
                     /inference="COORDINATES: protein motif:HMM:NF017273.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HNE05737.1"
                     /translation="MNNIWLVMIVGGLITFSMRFSLIYLFGKFEIPESLRRALHYVPP
                     AVLSAIIFPEIFLSNGALDISLSNARLPAGILAIVAAWYSRSTLVTILIGMAALFFFQ
                     WIKL"
     gene            complement(893..1786)
                     /locus_tag="PLT08_14520"
     CDS             complement(893..1786)
                     /locus_tag="PLT08_14520"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HNE05738.1"
                     /translation="MNILVLGTDSREVSAIQQIASGDGNTVVLASSSAEAWQVIQKGG
                     IQVLVSDIDATDFVPFQLISKVRTLKDSTYTLLITSRNDDGLLPKGDDILHRPYTSAD
                     LKNRIEIAKRFIALTAKLVIVSQQLENQAAFDPLTGFLNRAGFLRQATGELERSRRSS
                     SPLCLIALDIDNFQVINDRFGSKVGDDVLEAVAKSIRDKSRPYDCIGRWTGDEFVIVI
                     PNIIGADAEKVAERIIAGVRGTRIEVADEPPLNVKISAGIAAIMRITSTTEVEPLIQN
                     ARQALTRAKEAGGNQVYLSFL"
     gene            1895..1966
                     /locus_tag="PLT08_14525"
     tRNA            1895..1966
                     /locus_tag="PLT08_14525"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1927..1929,aa:Met,seq:cat)
     gene            2028..>2329
                     /locus_tag="PLT08_14530"
     CDS             2028..>2329
                     /locus_tag="PLT08_14530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE05739.1"
                     /translation="MCALNTQDQGIIVLPQQDYLPILIESFLIDRRSQGLSPGTIEFY
                     TKKLKYFQQYCEGFALTQVSQLTSDFIRRYILEVSETHNPGGVHACFRPLRTMLYW"
BASE COUNT          567 a          608 c          493 g          661 t
ORIGIN      
        1 ccgcacagtt aatgtcctcc attgtatttg ccgggtcggc acaattcatt acggcgcagt
       61 tatttagcga aggcgcgccc gctttcgtca tgatccttac cattgcagtc gtcaatttac
      121 gccatatgtt gtacagtgcg tctcttgcgc cctatctcaa agatttatcg ttacgttgga
      181 aggttcttct ttcttactta ttgacagacg aagcctatgc tccgtctatt ttgcattatg
      241 aaaaagaagg gcttgctaaa tacaaatact ggttcttatt tggcgcgggt ctctctctct
      301 ggataaactg gcagatcagc acagccattg gcgtgttcct tggtgcagcc atccctgaga
      361 acctatctct cgatttcgct ctgccagtta cattcatcgc catgatcgtg cccgtgatga
      421 aaaagtttcc agtgattgcc gccgcattaa gtgcaggcat cactgcttta ctagcttatc
      481 atctgccata taaactcggt ttgattcttg ccgccttggt gggcattacg gttggaatgt
      541 tgcttgaaaa tcttcaagct gcccaaaagg aggcaaaatg aacaacattt ggctggtcat
      601 gatcgtcggt ggcttgatca ctttcagcat gcgtttttcc ctcatttatt tgttcggaaa
      661 atttgaaatt cctgaaagct tacgccgcgc cttacattac gtcccccctg ccgtgctttc
      721 tgccattatt tttcccgaaa tctttttaag caacggcgcg ctggatattt ctttatccaa
      781 tgccaggcta cctgctggta tcttagcaat cgtcgccgca tggtactcac gcagcacgct
      841 cgtcactatt ctgattggaa tggcagcact atttttcttt caatggatca agctatagga
      901 aagacaaata cacctgattg ccccccgcct cttttgcgcg cgtcagagct tggcgcgcgt
      961 tttgtatgag cggctccact tcggttgttg atgtgattcg catgatggca gcaatacccg
     1021 cactaatttt gacattcaaa ggcggctcat ccgcgacctc gatgcgggtg ccccgaaccc
     1081 cagcaataat tctctctgcc accttttccg catccgcacc aatgatgttt ggaatcacaa
     1141 tcacaaattc atcgccggtc caacgtccaa tacaatcata tggtcggctc ttatcgcgaa
     1201 tcgacttggc tactgcttct aaaacatcat cgccaacctt cgaaccaaat cgatcattga
     1261 ttacctgaaa gttatcaata tccaatgcaa ttaagcataa gggagaagaa gagcgccgag
     1321 accgttccaa ctcgcctgtt gcctgacgta aaaacccagc tcggttcaag aagccagtta
     1381 gcgggtcaaa cgccgcctgg ttctctagct gttgactgac gatgacaagt ttggctgtca
     1441 acgcaataaa ccgcttggca atttcgatgc ggttctttaa atccgctgag gtgtaagggc
     1501 ggtgaagaat atcgtcgcct ttggggagca atccatcgtc atttctagat gtaatcaaca
     1561 gagtgtaagt cgagtctttt agagtgcgaa cttttgaaat tagctggaaa ggcacaaaat
     1621 ctgtggcatc tatatcggac accagaacct gaatgccacc cttttgaatg acctgccacg
     1681 cctcagcact ggaagatgcc aaaacaaccg tgtttccatc tccgctcgca atttgctgaa
     1741 tggctgaaac ttccctagaa tccgttccta atacaaggat gttcatggat gcacctttgc
     1801 cccattatag caggtgacct cgcttctttg acggttttcc acccacgttg acaatttgca
     1861 tcccccatag taatattgcg tccgctttga ccacgccgag gtagctcagt ggcagagcag
     1921 gggactcata agcccttggt cgggagttca aatctccccc tcggcacacc gtttcctagt
     1981 gaaacactct tcttatatat ggtggtctct acacaaagga gatcaccatg tgtgctttaa
     2041 atacacaaga ccaggggatt atcgtactcc cgcaacagga ttatctgccc atcctgatcg
     2101 agtcttttct aatcgaccgc agatcacaag ggctatcccc aggaaccatt gagttctata
     2161 ccaagaaatt aaagtacttc caacaatatt gtgaagggtt cgccctgaca caagtctctc
     2221 aacttacatc ggatttcatt cgtagataca ttcttgaggt ctctgaaact cacaacccag
     2281 gtggtgtaca tgcttgcttt cgcccactgc gcactatgtt atattggat
//
LOCUS       DAOQJE010000636         2329 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_34528_length_2329_cov_4.328056, whole genome shotgun sequence.
ACCESSION   DAOQJE010000636 DAOQJE010000000
VERSION     DAOQJE010000636.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2329)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2329)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2329
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34528_length_2329_cov_4.328056"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..186)
                     /locus_tag="PLT08_14535"
     CDS             complement(<1..186)
                     /locus_tag="PLT08_14535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE05740.1"
                     /translation="MTALDEPTPMGIGAIPGYVALVDFHLPSLPKIKAQQGQTSTVTI
                     AQSCAPYGTVRDTSHLTP"
     gene            complement(209..1339)
                     /locus_tag="PLT08_14540"
     CDS             complement(209..1339)
                     /locus_tag="PLT08_14540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE05741.1"
                     /translation="MKKTITNILLSAFVLCSCAQGSAPFPTVTLPYLIATTSTATEFS
                     TATNDATDASVFTDLELSCETFVETRWGNGVNEFGFNENPPNYQSPGPATPTFDVQGN
                     MFIADWANERILVYPKGEKTPKIIHVPDLYEPEFLPGTDYWWSNIIVSMDRIFFLTVG
                     VKDKRNIPRLFALSLKDNGLTKIDLEPYYSPTTNSARLLEDTHGGVYIFMMNFLVHYD
                     KDLHPDLLASDELEKFFYIVRGWDGNLYTAGEPDVIKNWGVDARFNEYPEYVFQDVHA
                     TLTGSKDKSVHYIPIGADSAGKLYFVRFFQLYDEAKNEVDIIRFDVQTRTGEFGKIKN
                     KEWLSYYHDYVLAPDGSLYSLIYAPKDSSVQPKIIKCSFGSL"
     gene            complement(1421..>2329)
                     /locus_tag="PLT08_14545"
     CDS             complement(1421..>2329)
                     /locus_tag="PLT08_14545"
                     /inference="COORDINATES: protein motif:HMM:TIGR03696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
                     /protein_id="HNE05742.1"
                     /translation="ANGAAQSAYGFTGEMADVSGLTYLRARYYSPLDGRFMSRDTWEG
                     NSYHPITLNKWSYAHGDPVKFTDPSGHDPWWCEYSSNPKQCEEDYWNSWNIRCSTPEP
                     TPPSTLTEWEIAYGQAIANAAKEIYANRDSYYVWGAIHTDDEWYVNPPPREIPADGFI
                     WNGIVNVKEQTNGRIPIVCADIIAMSYENAGYKLSATFPVWANNSGYTTDPERSVPAL
                     YNLLSIQNYWNVPRIDTSPQKFELGDIVINGDMTHSAVIVEVNGARDDASQTFVVQAS
                     YRLNPGEITRMSLAEWSMNAGWYGSP"
BASE COUNT          642 a          534 c          478 g          675 t
ORIGIN      
        1 aggggtcaag tgcgaggtgt cacgtactgt cccatacggg gcgcaggatt gggcaatggt
       61 gacggtggaa gtctgtccct gttgggcttt gattttcggc agggagggca ggtggaagtc
      121 cacgagcgcg acgtagccgg ggatcgcgcc gattcccatc ggggtcggtt cgtccaatgc
      181 ggtcaaatcg ttaaacgccc acagtcaact ataagctacc gaatgaacac ttgataattt
      241 tgggttgaac agaagaatct ttgggagcat aaattaggct atacaacgat ccatccgggg
      301 caagcacata atcatgataa tatgataacc attctttgtt tttaatcttg ccaaactccc
      361 cagtgcgcgt ttgaacatca aatcgaataa tatccacctc attttttgcc tcatcataaa
      421 gttggaaaaa tcgtacaaaa tatagctttc ctgcagaatc tgctccaatt ggtatgtagt
      481 gtacagactt gtctttcgaa cctgtcagtg tagcatgaac atcctgaaaa acatattctg
      541 gatactcatt aaatcttgcg tcaacgcccc agttcttaat tacatcaggt tcgcccgcgg
      601 tgtacagatt accatcccag cctctaacaa tatagaaaaa tttttcaagt tcgtcactgg
      661 caagtaaatc tgggtgcaaa tctttgtcgt aatgaacaag aaaattcatc ataaaaatgt
      721 acaccccgcc atgtgtatct tcaagcagtc gggcagaatt ggttgttggg gaatagtaag
      781 gttctaggtc aattttcgtt aagccgttat ccttaagaga caaagcaaat agtcggggaa
      841 tatttctttt atctttaact cctacagtga gaaaaaaaat tctatccata gatacgatga
      901 tgtttgacca ccaatagtct gttccgggaa gaaattcggg ttcatataaa tcgggcacat
      961 gaattatttt tggagttttt tctccctttg gatacaccaa aattctttcg tttgcccagt
     1021 ctgctatgaa catattgccc tgcacatcaa acgtaggagt ggcagggcca ggtgattggt
     1081 aattaggagg attttcatta aacccgaact catttacccc attcccccat ctagtttcca
     1141 caaatgtttc gcaagataat tccagatcag taaatactga agcgtccgtc gcgtcattcg
     1201 ttgcagtgga aaattctgtt gcagttgatg ttgtcgcaat taggtatggt aatgtgactg
     1261 ttggaaatgg agccgaacct tgcgcacaac tgcataatac aaacgcactt aatagaatat
     1321 ttgtgatcgt ttttttcatg ttttgccttc tttcaaaacg tgctttttcg caagcgcgtt
     1381 gcacgtgcaa cgcgcttgcg aaattctctg tccaaatcaa ctagggcgac ccataccaac
     1441 cagcgttcat agaccattct gccaatgaca ttcgtgttat ctcgccaggg ttcaaacggt
     1501 aacttgcctg aactacaaaa gtttgagaag catcatcacg agcgccatta acttctacaa
     1561 ttaccgccga atgagtcata tctccgttta tcacaatatc acctaattca aatttctggg
     1621 gagatgtatc aattcttgga acattccaat aattttgtat acttaataga ttgtacaatg
     1681 ctggcacact cctttcagga tctgttgtat agccgctatt attagcccaa acaggaaaag
     1741 ttgcacttaa cttatagcca gcgttttcat atgacatagc gatgatatct gcacacacaa
     1801 ttggaatacg accatttgtc tgttctttta cattaacaat gccattccag ataaaaccat
     1861 cggcaggaat ttcccttggc ggcggattta cataccattc atcatcagta tgtattgcac
     1921 cccaaacata gtagctgtca cgatttgcat aaatttcttt ggccgcattt gcgattgctt
     1981 gtccgtacgc tatctcccat tctgttagtg tcgatggtgg cgtcggctct ggagtagaac
     2041 aacgtatatt ccaagagttc caataatctt cctcgcattg tttagggttg cttgaatatt
     2101 cacaccacca aggatcgtga ccagacgggt cagtgaattt cacaggatcg ccatgagcat
     2161 agctccactt attgagagtg attgggtgat aagaattccc ctcccatgta tcccttgaca
     2221 taaacctccc atccagcgga ctgtaatacc gcgcccgcag atacgtcaac ccgctgacat
     2281 ccgccatctc acccgtgaat ccatacgcgg attgagccgc tccgttggc
//
LOCUS       DAOQJE010000637         2326 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_34609_length_2326_cov_5.640687, whole genome shotgun sequence.
ACCESSION   DAOQJE010000637 DAOQJE010000000
VERSION     DAOQJE010000637.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2326)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2326)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2326
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34609_length_2326_cov_5.640687"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2322
                     /locus_tag="PLT08_14550"
     CDS             <1..2322
                     /locus_tag="PLT08_14550"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HNE05743.1"
                     /translation="KWIAIASLRDLAMEKHNEEFFRSTAIASGENPNPVFRISPDGKI
                     IFSNTSAEEMLKSIGHHEQNIPAAWVNSLQQALDSGTQVVSIIQHGERAYSCVFAPAP
                     TKEYVDMYALNVTDREEEKARLALSDEILNSIGNLVMVANSNAEIVYISPSVTNLLGY
                     RPDEILGEGWWEIERISGGDVQVEKEYIRKAAAGKIDVDGKPYEHHVRHKDGSWRWLM
                     LSDTKGPRDLVIGIGYDITPIKAAEEELERQRDFARTLTNQMGQGLTVTDENGCFEFV
                     NPSYAQMLDYEPSELIGKTPYDFTLPEDHWNLLNAQVKRSLGSVTTYETRLLGRNNKI
                     VYALITGVPRSVNGNYKGAITVVTDLTERLTMEKQLRQYADEIQQANIQLADARDRAL
                     EASYLKSAFLATMSHEIRTPMNAILGMSELLLDTELNEEQREFANVIEASTQNLLAIL
                     NDILDLSKIEAGKLAIHPAPFNPASVIMETMKLFSPKAQQKNINVSVNVPPDAQTLVL
                     GDAGRIRQVLSNLLSNAIKFTERGGAVFVTLSSSQIKDVMMTTFTVQDTGLGIPDAVK
                     PKLFNPFTQADDSQTRRYGGSGLGLAISKRLIDLMHGEIGFETLEGKGSTFWFSLPLT
                     MINETTEVVAEKQMPRQKDPLPFVVTKPALIVEDNLLNRDLISLQLRELGLNASYATN
                     GKEAVELLKFQPDEYSIVLMDMNMPVMDGTAATKLIRENEAGTTRHVPIIAVTANVMT
                     GTREFCLQAGMDDYLSKPITLQSMREVLEKWLV"
BASE COUNT          682 a          464 c          555 g          625 t
ORIGIN      
        1 aaatggatcg ctattgcttc attgcgagac cttgccatgg aaaaacataa tgaagaattt
       61 ttccgctcca cggcaattgc ttcgggtgag aacccaaacc ctgttttccg tatcagccct
      121 gatgggaaga ttatcttttc gaataccagt gctgaagaga tgttaaagag cattgggcat
      181 catgagcaga atatccctgc tgcttgggtc aattccctgc aacaagcact cgatagtgga
      241 acacaagtgg tgtctattat tcagcacggg gaaagggcat attcttgcgt ttttgctcca
      301 gcgcccacaa aagaatatgt ggatatgtat gcactaaatg ttacagatcg ggaagaagag
      361 aaagcgcgcc tcgctttatc tgacgagata ttgaattcaa tcggcaattt ggttatggtt
      421 gcaaatagca acgctgagat cgtctatatc agcccttctg tgacaaacct tcttgggtat
      481 cgtcctgatg agattttagg agagggctgg tgggagattg aacgcatctc tgggggagat
      541 gtgcaagtag agaaggaata tatccgtaag gcagcagccg gtaagattga tgtagacggc
      601 aaaccgtatg agcatcatgt acgccataaa gacggttcat ggcgttggtt gatgttgtct
      661 gacacgaaag gtccccgaga cctcgtgatc ggaattgggt atgacattac ccccatcaaa
      721 gcagcggaag aggaactcga acgtcaacgt gattttgctc gaacccttac caatcaaatg
      781 ggacagggat taactgttac agacgaaaat ggctgtttcg aatttgttaa tccttcttat
      841 gcacagatgt tggattatga gccatcagaa ttgatcggta aaacacccta tgactttacc
      901 cttccagagg atcactggaa tttgttaaat gctcaagtaa aacgttcttt gggcagtgta
      961 acaacatatg agacccgttt gctcggacgt aacaataaga ttgtttatgc tctgatcacc
     1021 ggtgtgccac gcagcgtgaa tggaaattat aaaggtgcaa tcaccgtagt aaccgattta
     1081 acggaacggt taaccatgga aaaacaatta cgccaatatg ctgatgaaat tcagcaggca
     1141 aatattcaat tagcagatgc gcgtgaccgg gcacttgaag cttcttattt gaaaagcgct
     1201 tttctcgcaa ccatgagtca cgaaattcga acaccgatga atgctatttt ggggatgagt
     1261 gaactgttat tagatactga actaaatgaa gagcaaagag aatttgcgaa tgtcattgaa
     1321 gcttctaccc agaatttatt ggcgatctta aatgacattt tagatctttc caagatcgaa
     1381 gctggaaagt tggcgatcca tcctgcaccg tttaatcctg cctcagtaat catggaaacc
     1441 atgaagctct ttagccctaa agcacagcaa aagaatatca atgtttctgt taatgttccg
     1501 cctgatgctc aaactctagt cttaggagat gctggaagaa tccgccaggt attgagcaac
     1561 ctcttgagca acgctatcaa attcacagaa cgtggaggcg ctgtttttgt cacactctca
     1621 agctcacaga tcaaagatgt gatgatgacc acttttacag tccaagatac ggggttgggc
     1681 atccctgatg cagtcaagcc aaaattattt aatccattta ctcaagcgga tgattctcag
     1741 acgcggcgtt atggagggtc tggtttgggg ctggcaattt caaaacgctt gatcgatctc
     1801 atgcatggag aaatcggttt tgagacattg gaagggaagg gttccacctt ctggtttagc
     1861 ttgccattaa ccatgataaa tgaaacgacc gaagtggttg cagaaaagca gatgcctcgg
     1921 caaaaagacc ctttgccatt tgtcgtgacg aaaccagcgc tgattgtaga agataacttg
     1981 cttaatcgag acttgatttc acttcaatta cgagaattag gcttgaatgc cagttatgct
     2041 accaatggaa aagaagcggt cgagttattg aaattccaac ccgatgaata ttccatcgtt
     2101 ttgatggata tgaatatgcc tgttatggat ggaacggctg ctacaaagtt gatccgcgag
     2161 aatgaggcgg ggacaacaag gcatgtgcct attattgctg tcactgcaaa tgtgatgaca
     2221 ggcacccgtg aattttgttt gcaagctggt atggatgatt acctgagcaa accgatcacg
     2281 ttacagtcta tgcgtgaagt cttagaaaaa tggcttgttt gatata
//
LOCUS       DAOQJE010000638         2314 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_34940_length_2314_cov_6.366091, whole genome shotgun sequence.
ACCESSION   DAOQJE010000638 DAOQJE010000000
VERSION     DAOQJE010000638.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2314)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2314)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2314
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34940_length_2314_cov_6.366091"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1954)
                     /locus_tag="PLT08_14555"
     CDS             complement(<1..1954)
                     /locus_tag="PLT08_14555"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HNE05744.1"
                     /translation="MKNYQLPLRILSIVVVLIIAGMTRARAVQHLNIDFDEDDYTRAA
                     QEFAHLIRTSNWSGFLETNYRPEHPPLAKIVMGISLLTAPEKDLIADRPTSAEPNKYL
                     PRVILKPERTVNAVFGWLTVALLAIVNPLAGLSLATHSITVKYNSQAMLEALPALTSF
                     CMVLAYLQFKKNHNTRWLITSAVFLGLTAASKYLYCVIALAILADWFLTSKEQEELKS
                     FFKQALIWGTLGILLFFVTNPFLWANPIQRLRESVLYHAGYSSSSEEVKDAGFPVWQP
                     VQWLVTSAADWEEDAFFTGPDLLIAILAAFGFARLWKRERLYVLWFGFALLFLLLWST
                     KWPQYILILTVPLSLAAAEGLTQIWQNILEGLKAPKTVYNKKESRQAIPWLVPGLIVF
                     TVLTLLPFFFQLGVSLTDFSSQSIRDGFQGGIWREVSGGLTGQITPTSPEVAYNSNKV
                     QYIGFQAYQPVFEYIVSVNKIFVFNILWMVVSTSIQVLLGLGLAILLNQRGVKLGKFW
                     QTLFILPWAIPEFIGALMWLNIFVPEIGWLSLAAKKYGDAFPLQSLIGWQSTPSLWFL
                     VLLIPAIWYGTPLIMLASNASLKTIPKEIFEAASLDGANSWQILQHMVLPLIRPVLLP
                     AIIIRGIYAFNQFYLFQAMPFSEGTLA"
     gene            complement(2051..>2314)
                     /locus_tag="PLT08_14560"
     CDS             complement(2051..>2314)
                     /locus_tag="PLT08_14560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HNE05745.1"
                     /translation="ARLFEIEEAKRQAELEADRRSQVGSGDRSEKIRTYNYPQSRVTD
                     HRIGLSNYNLAGVMDGDIEGFIDELTTRDEAGKLASTGVEDDE"
BASE COUNT          650 a          502 c          591 g          571 t
ORIGIN      
        1 ttgccagagt accttcgctg aatggcatag cttgaaatag atagaactga ttgaaagcat
       61 aaatgccgcg gatgataatg gcaggtaaaa gaacaggtct tatcaacggt aagaccatat
      121 gctgcaagat ttgccagcta ttggctccat caagagaggc ggcttcaaag atttcttttg
      181 gtatcgtttt gagactcgca tttgaggcaa gcataatcaa aggtgttccg taccaaatgg
      241 caggaataag taagactaag aaccaaagac tgggtgtgct ttgccaacca attagagact
      301 gcagtggaaa ggcatcgcca tactttttgg cggcaaggct taaccaccca atttctggca
      361 caaagatgtt cagccacatc aaagcgccaa taaattcagg aattgcccaa ggcagaatga
      421 aaagagtctg ccagaacttg ccaagtttta ccccgcgttg gttaagcaaa atagcaagcc
      481 ctaaccctaa aaggacttgg atactcgtag aaaccaccat ccaaaggatg ttgaaaacaa
      541 aaattttatt tactgaaact atatactcga atacaggttg ataagcttgg aaaccaatat
      601 attgaacttt atttgaattg taagcaactt caggagatgt tggtgtaatt tgtcctgtaa
      661 gcccaccact tacttcgcgc cagatgccgc cttggaatcc atcacggatg gactggctgc
      721 taaagtctgt aagtgaaacg ccaagttgaa agaaaaacgg aagcagggtt aaaacagtaa
      781 acacaatcaa gcccggcaca agccagggaa tagcttggcg tgattctttt ttgttgtaca
      841 cagtttttgg ggctttgagc ccttcaagaa tgttttgcca tatttgtgtg agaccttctg
      901 cggcggcgag cgaaagcggg acggtgagga tcagaatgta ttgcggccac ttggtagacc
      961 ataaaagcaa aaacaacaag gcaaagccga accacaatac atacaaacgt tctctcttcc
     1021 acaatcgtgc aaacccaaat gcggcaagga ttgcaatcag aagatctggt cctgtaaaga
     1081 aagcatcttc ttcccagtct gcggcgctgg ttaccaacca ttggacaggc tgccaaacgg
     1141 gaaacccagc atctttgact tcttctgaac ttgaagaata gccagcatga taaagaaccg
     1201 attcacgcag tctttgaatt ggatttgccc agaggaatgg attcgtcacg aagaagagaa
     1261 gaatccctaa cgtgccccag atcaaggctt gcttgaagaa tgatttgagc tcttcttgtt
     1321 ctttcgatgt cagaaaccaa tccgcgagaa tagcgagggc aatgacacaa taaagatact
     1381 ttgaggcggc agtaagacca agaaagacgg ccgatgtaat taaccagcgg gtattgtgat
     1441 tctttttgaa ttgtaagtac gccaatacca tgcagaaact ggtcaatgca ggcaatgcct
     1501 ctagcatggc ttgcgagttg tatttaacag tgatgctatg cgtagcgaga ctcaacccag
     1561 ccagaggatt gacgattgcc agcaacgcca cagttagcca gccgaagacg gcgttgaccg
     1621 tccgctcggg cttgaggatg acacgcggca aatatttatt gggttcagct gaagtggggc
     1681 ggtctgcgat caggtctttt tctggggcag tgagcaggct aatgcccatg acgatctttg
     1741 ccagcggggg gtgctcaggg cgatagttcg tttccagaaa gccagaccag tttgaggtcc
     1801 ggatgagatg cgcgaattct tgagcggcgc gagtgtagtc gtcttcgtca aagtcaatgt
     1861 tgaggtgttg aactgcgcgg gcgcgagtca tcccagcaat gataaggact acaacaatgc
     1921 tcaagatgcg aagggggagt tggtagttct tcatggcgat ggattgtatc ctcttcttcc
     1981 gcctgaattg taataccgta gagtggatta ttgaaaacag gctggagttc tccagcctgt
     2041 tgtgagaatt ttattcgtcg tcttcgacgc cagtggatgc gagcttccca gcttcatcac
     2101 gtgtggtgag ttcgtcgatg aagccttcga tgtcaccgtc catcacaccg gcgaggttgt
     2161 aattcgataa cccgatgcgg tgatctgtaa cgcggctttg tggatagttg taggtacgga
     2221 ttttttcgga gcgatcgccc gagccgactt gcgagcgacg gtctgcttcg agttcggctt
     2281 ggcgtttggc ttcttcaatt tcaaacaaac gagc
//
LOCUS       DAOQJE010000639         2314 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_34941_length_2314_cov_5.635680, whole genome shotgun sequence.
ACCESSION   DAOQJE010000639 DAOQJE010000000
VERSION     DAOQJE010000639.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2314)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2314)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2314
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34941_length_2314_cov_5.635680"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..349
                     /locus_tag="PLT08_14565"
     CDS             <1..349
                     /locus_tag="PLT08_14565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE05746.1"
                     /translation="EVRDSQMLVFGWMFLPLRGGKLPESILDKFNPIVPKAIHEKIIK
                     RSAKGFLIGCIVGGIPMCLGISTALGTPFVNLSYDWAVLMAIGFSLMGITGTSIACSA
                     LFGGNLEYAENKQ"
     gene            539..814
                     /locus_tag="PLT08_14570"
     CDS             539..814
                     /locus_tag="PLT08_14570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE05747.1"
                     /translation="MNKSNERNNTLKNIWSSFLAGFGSAVLGFFAGTVLSGVLIFIFF
                     SIFGTFTEVGPLMVMYVSPIIGVVTAVFLGIRSGIKIYKSSSASNNQ"
     gene            881..1171
                     /locus_tag="PLT08_14575"
     CDS             881..1171
                     /locus_tag="PLT08_14575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE05748.1"
                     /translation="MKSGIVSVVAGMLGASLTVAVMDFTFGLDVAIKSFFKVLPRSIC
                     IGGVPSLILAAIIFAFWKKSGKNEKGMIETCLITGFIVGVLCGVPLYISYGM"
     gene            1352..2179
                     /locus_tag="PLT08_14580"
     CDS             1352..2179
                     /locus_tag="PLT08_14580"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024917.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNE05749.1"
                     /translation="MIENNILINPTSNEIASAVHENLYALFRSMQVIPNYELAENDCL
                     GLHHAPLSNPMFMGAWKANFSLDNVENEIDEVLSWFKKRNAPSFFWWTDIQTQPAGLV
                     EHLLKRGFDGNLEGDPGMAVNLHEINENIKLPNSLEIVWAKDNKTLVDWRNIFAEAFE
                     SPVAAGQAWVDATTSTSIENSPWQLYVGYLNQQPVSISILFNGAGVSGIYGVGTIPTH
                     RNKGIGAAMTLIPLLQARNQGYHFGVLFSSRMGYSVYKRLGFYEVGNNIGLFMKEWD"
BASE COUNT          656 a          457 c          521 g          680 t
ORIGIN      
        1 ggaagtcaga gattcgcaaa tgttagtttt tgggtggatg tttctcccat tgcgtggagg
       61 aaaattacct gagagcattt tggataaatt caacccaata gtgccaaaag ccatccatga
      121 gaaaatcata aaaagaagtg caaaaggatt tctgatcgga tgcatcgtgg gtggtattcc
      181 aatgtgttta ggaataagca ccgctctagg taccccattc gtaaacctgt catatgattg
      241 ggcagttctt atggcaatag gcttttctct catgggcata actggtacat caattgcttg
      301 ctcagcacta tttggtggta atttagaata tgcagaaaat aaacaataaa tcaatacgta
      361 ataacatctg cccaacaaag cgtgcacctg accagcggga gtctgcggca ttttcaagca
      421 ttttccacgc ttcggcagaa tcctgctctc gaagttttat ctacgcccgc ccgctggtca
      481 ggtaacgcaa accgttagcc cgctgcgccg tgagcagtag tattaaaaag gattactcat
      541 gaataagtct aatgagagaa ataatacctt gaagaatata tggtcaagtt ttcttgctgg
      601 tttcggatct gcggttctcg gcttctttgc tggcactgtt ttatctggcg tgctcatttt
      661 catctttttc agtatatttg gaacatttac agaagttggt ccattgatgg tgatgtatgt
      721 ttccccaatt attggggttg ttacagccgt ctttctcggc ataagaagcg ggataaagat
      781 atataaaagt agcagtgcat caaacaatca gtaagtcttc aatcaaaact tgttccagat
      841 gtttcgtcac aaaggagttt aatgatttat gaacatatac atgaaatcag gtattgtgag
      901 cgtagtggca ggaatgcttg gggcgagtct caccgttgca gttatggatt ttacattcgg
      961 gctagatgtg gctatcaaaa gtttcttcaa ggtgcttcct agaagtattt gcatcggcgg
     1021 tgttccaagt ttaattttag cggcaatcat ctttgctttt tggaaaaagt ctggaaaaaa
     1081 tgaaaaaggc atgatagaaa catgcctcat aacaggtttc attgttggtg tattatgtgg
     1141 agtaccgctt tatatttctt atgggatgta gttactgaag acaaaaagag acaaaaagcg
     1201 ggctaacaaa gcgtgcacct gacgctgggg attctgcgca catcccaagc atttttctac
     1261 gccttagcat ttttctggtc ggacggcttc gctgtcccca ccccagcgca ggtaacgcat
     1321 gccgttgggc gttgaaaagg aagcagacat catgatcgaa aacaatattc ttattaatcc
     1381 aacaagtaat gaaatcgcat ctgccgtcca tgaaaacttg tatgctcttt tccgctccat
     1441 gcaagtcata ccaaattacg aattggcaga aaatgattgt cttgggttgc atcatgcacc
     1501 actttctaat cctatgttca tgggtgcatg gaaagcaaat ttttctttag acaatgttga
     1561 aaatgaaatt gatgaagttt tgtcttggtt caagaaacgc aatgcaccat cttttttttg
     1621 gtggactgat attcaaactc agcctgctgg gttagttgaa catttgctaa aacggggttt
     1681 cgacggcaat ttagaaggcg accctggtat ggctgtcaat cttcatgaaa tcaatgaaaa
     1741 tatcaaatta ccaaacagtc ttgagattgt ttgggcaaag gataataaaa cattggtgga
     1801 ttggcgcaac attttcgctg aagcatttga aagcccagtt gcggctggac aagcatgggt
     1861 agacgcgaca acatctactt ccatagaaaa ttcgccttgg caattgtatg ttggttatct
     1921 taaccagcaa ccagtatcaa tcagtatcct ctttaatggc gctggagtat caggaatata
     1981 tggagtaggc accataccaa cacatcgcaa caaaggcatt ggtgcagcaa tgacattaat
     2041 tccattactt caagccagaa atcagggata ccattttggc gtcttgtttt catcgcgaat
     2101 gggctattct gtttataagc ggttagggtt ttatgaagtc ggaaataata ttggcttgtt
     2161 tatgaaagaa tgggattgat gatcaaaaac gcccaacaaa gcgtgcacct gacgctgggg
     2221 attctgcgcc gttttcaagc agtttcctac gccttagcct tttctggttg gacggcttcg
     2281 ccgtccccac cccagcgcag gtaacgcaaa ccgt
//
LOCUS       DAOQJE010000640         2313 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_34968_length_2313_cov_5.198849, whole genome shotgun sequence.
ACCESSION   DAOQJE010000640 DAOQJE010000000
VERSION     DAOQJE010000640.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2313)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2313)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2313
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34968_length_2313_cov_5.198849"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..308
                     /locus_tag="PLT08_14585"
     CDS             <1..308
                     /locus_tag="PLT08_14585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE05750.1"
                     /translation="RLELDRYLARHHGDTSQPMPIGSPYVFQNNGEFVAGYLEAFFRN
                     PHYIASQNEILFSAYVELFGYDPRKAWEEDFPHYVKKNREFYLSGKRPPKTGLSMPK"
     gene            347..1879
                     /locus_tag="PLT08_14590"
     CDS             347..1879
                     /locus_tag="PLT08_14590"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HNE05751.1"
                     /translation="MTIVKTFFQRTWEYFFVLIGVLGVLLPVSPLNMRLVYRDSGVFL
                     YTGWRILSGDLPYLNIWDHKPPVVYYINALGLALTHNSRWGVWLIEMVALYIAAYIAY
                     SLIKNIFGQIPALMSSLFWLMSLVLILQGGNFTTEYTLPLQFLALWLFAKLDIYSSQK
                     SYFFLTGLTGAFAFFTKQTTIGLWVGFFIYLAFYRLSQKQGRQYLSEALWMVLGGLSI
                     TLFVFVFFWANGAISQFWDAAFLYNFTYSERIDSWLPPFSIIWKSLRPLMLSGILPLA
                     ITGLVLSVIVFWKNKEKNLFHLVRICIVTFPIELILIFMPSKTFPHYFITLLPTLAIF
                     TAATFYSVSKAATSIKIPNSAQYLFFAGIAIIASWNFFHNYLNQVYIYRKLNDDALIQ
                     YTISNTAQDDTVLVWGADPSINYFSQRESPTRYIYQFPLQEENYVTETMIIDFLDDII
                     QKQPKLVINIDPDHSLFTFPVESEEILARAKTLEEMYCLVDVLDSAPIYSLRQGNSCL
                     NE"
     gene            complement(1888..>2313)
                     /locus_tag="PLT08_14595"
     CDS             complement(1888..>2313)
                     /locus_tag="PLT08_14595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE05752.1"
                     /translation="FHFLLDHLLWIQIAKQGKILHVNQTWAAARYHAEAKNVSKAAEF
                     GREAFRILETIAQDKDLAKALLSVDRRAHASAFRVDARYLLDGGYPAKALQQWFRALF
                     IHPPTALGRINIFVSSLLNLLGLSFMRRKILEIREKRHQ"
BASE COUNT          648 a          485 c          442 g          738 t
ORIGIN      
        1 aacgcctcga actcgaccga tatctggctc gccaccatgg agatacctct caaccgatgc
       61 cgatcggtag cccatatgtc tttcaaaaca atggcgagtt tgtggctggt tatttagaag
      121 cgttttttcg taacccacat tacatcgctt cgcaaaatga gatcttattt agcgcctatg
      181 tggagctctt cggctatgac ccgcgtaagg catgggaaga agattttcca cattatgtca
      241 aaaagaatcg agaattctac ttaagcggga aacgtccgcc caaaacgggg ctgagtatgc
      301 ctaaataatc gtttagaggc tttggtatac tcatttcaga gctaatatga caattgtaaa
      361 aacatttttt caacgcacat gggagtattt ctttgtgctt attggtgtct tgggggttct
      421 tttgcctgtc agcccattaa atatgaggtt ggtataccgc gactctggcg tgtttttgta
      481 tacgggttgg cgtatattaa gtggagattt accatatctc aatatatggg atcataaacc
      541 acccgttgtt tattacatca atgcattagg tttagcttta acacataact cccgttgggg
      601 agtatggctt atcgaaatgg ttgcgcttta tatagcagcc tatatagcat acagcttaat
      661 aaagaatatc tttggtcaaa ttccagcgtt gatgtcctcg ttattttggc ttatgtcgct
      721 ggtgctcatt ttacaaggcg gaaactttac cactgagtac acacttccac tacagtttct
      781 agctctttgg ctttttgcga aattagacat atactcatcc caaaaaagtt atttttttct
      841 aacaggtctt actggagctt tcgctttttt taccaaacaa accacaatcg gtctctgggt
      901 gggatttttc atttacctag ctttctatag gctttctcaa aaacaaggaa ggcaatatct
      961 ttctgaagca ttatggatgg tcttaggcgg actatccatc actctatttg tgtttgtttt
     1021 cttttgggca aatggggcta tctcgcaatt ctgggatgca gcatttcttt acaacttcac
     1081 ttattctgaa cgtattgata gctggctacc tcctttctca attatctgga agagtctccg
     1141 cccactaatg ctaagcggga ttcttccatt agcaataaca gggcttgtac tatcagttat
     1201 agtcttttgg aaaaataaag aaaaaaatct atttcaccta gtcagaatat gcattgttac
     1261 attcccgata gagctaattc ttatttttat gccgtcaaaa acattccctc attactttat
     1321 tacactgctc cccaccttgg caatctttac agcggcaaca ttctatagtg tttcaaaagc
     1381 tgctaccagt atcaaaatac ctaactcggc tcaatatttg ttttttgcag gtattgccat
     1441 tatcgcaagc tggaatttct tccataatta cttaaatcag gtgtacattt acagaaagct
     1501 caatgatgac gcacttatcc aatatacaat ttcaaatact gctcaagatg ataccgtttt
     1561 ggtatggggt gcagacccat ctataaatta tttttcccaa agagaaagcc ccactcgcta
     1621 tatctatcaa tttccactac aagaagaaaa ttacgtcaca gaaacaatga ttattgattt
     1681 tttagatgac ataatccaga aacagccaaa gttagttatt aatatcgacc ctgaccattc
     1741 gcttttcaca ttccctgttg aatctgaaga aatattggca cgcgcaaaaa ctctggaaga
     1801 aatgtattgt ttggtcgatg ttttagatag cgcccctatc tattcattaa gacagggaaa
     1861 ttcctgtctt aatgaataag aattaactta ttgatgtctt ttctctctaa tttctagtat
     1921 ctttcttctc ataaaactca gacctaataa atttagcagg ctcgaaacaa aaatattgat
     1981 tcgccctaaa gctgttggcg gatgaatgaa caatgcgcgg aaccattgtt gcaatgcttt
     2041 ggcagggtag ccaccatcga gcaagtaacg tgcatccacg cggaaggcag aggcatgcgc
     2101 gcggcgatct acgcttagga gggctttagc aaggtcttta tcttgggcaa tggtctctag
     2161 aatacgaaac gcttcacgcc caaactcagc ggctttggaa acgtttttcg cctcagcatg
     2221 gtaacgagct gctgcccatg tttggttcac atgcaatatt tttccttgct tggcaatttg
     2281 tatccaaagt aaatgatcga gcaggaagtg aaa
//
LOCUS       DAOQJE010000641         2307 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_35144_length_2307_cov_4.404529, whole genome shotgun sequence.
ACCESSION   DAOQJE010000641 DAOQJE010000000
VERSION     DAOQJE010000641.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2307)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2307)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2307
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35144_length_2307_cov_4.404529"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            159..812
                     /locus_tag="PLT08_14600"
     CDS             159..812
                     /locus_tag="PLT08_14600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE05753.1"
                     /translation="MPRRDVPFLPNQYYHFYNRGNNRQTVFFEAENYIYFLKGVKQYL
                     LGTVEVIAYCLMPTHYHLLVRVLANEQTSEVFKTSEVSRPEVSRPEASKQVSLGMQKL
                     LISYTKAINKRFERTGALFQGQFQGKPVTTYSHLLTLCAYIHANPVKDGLAAAPEMWD
                     FSNYLEWLGLRNGTLVNREFIAENFGTGEAYKQFVADYLRNRQVDEEFRKYVEGLER"
     gene            923..1357
                     /locus_tag="PLT08_14605"
     CDS             923..1357
                     /locus_tag="PLT08_14605"
                     /inference="COORDINATES: protein motif:HMM:NF016232.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNE05754.1"
                     /translation="MEYEKFKKLLVNKEQPNVDFKLESHAFASKTEYFNAELVKDICA
                     MANNGNVSSYLIIGVSDDVKSFKSVQNQKLTDENVQSLCKTAIYPPPKIRFLKKRWSE
                     NVLHEHKDKDFVIIQIGPHPKQVFRLAKDFIDYKEKNLFQKK"
     gene            1416..2078
                     /locus_tag="PLT08_14610"
     CDS             1416..2078
                     /locus_tag="PLT08_14610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE05755.1"
                     /translation="MFSGDPINISEEAEARKQERFIFSNLSVSEKCSAIFGKMENFVG
                     NDLGYKKSTWHKSPFSFQVNWKQIGSTLFFINCFSCDKSFTKQSLDWFYSRKFFSFDY
                     IAKKDLPAEFNEYLETIKLIRRITLWAFLDGVSQNKVMANFPSMRKSEIQHHYYHGEW
                     QDNNDKNKKTIYLPSSNEMVLIDKIKSLPDFTETLEDVIKELEKDNKATVFAPEIKKA
                     IW"
BASE COUNT          698 a          375 c          570 g          664 t
ORIGIN      
        1 ggatggacga aggatggata gaggatgagc gggtgggatt ctttcgggta gccaaggggt
       61 gtgctatact tttttttgat gatggggtaa ctaggttgag accccgatac cagacttccg
      121 aagtctccaa gacttcggaa gtcttctgga ggactattat gccacgcaga gacgttccct
      181 ttttgcctaa ccagtattat cacttctaca accgcgggaa taaccgtcag acggtgtttt
      241 ttgaggcgga gaattacatc tattttttga agggcgtaaa gcagtatttg cttgggacgg
      301 tggaggtgat tgcgtattgc ctgatgccga cgcattatca tcttttggtg agggtacttg
      361 ctaatgagca gacttccgaa gtctttaaga cttcggaagt ctcaagaccg gaagtctcaa
      421 gaccggaagc ctcgaagcag gtgtctcttg gaatgcaaaa gcttttgatc tcttacacca
      481 aagccatcaa caagcgtttt gagcggacgg gtgcgctctt tcagggacag ttccagggca
      541 agccggtgac gacctacagc catttgctga ccttgtgcgc gtacatccat gccaacccgg
      601 tgaaggatgg cttggcggct gcgccagaga tgtgggactt ttcgaactat ttggagtggc
      661 tgggtttgcg caatgggacg ttggtgaacc gtgaatttat cgccgagaat tttgggacgg
      721 gggaggcgta taagcagttc gtggcggact atcttcgaaa ccggcaggtg gatgaggagt
      781 ttcggaagta tgtggagggg ttggagaggt aggttgttag cgagacctcg gaggtcttaa
      841 agctcgggta aaattggaga aaatagggag aggattagtg aaagagttta agtattttat
      901 agctgacata taggtggtga taatggaata cgaaaaattt aaaaaacttt tagtaaataa
      961 agagcaaccg aatgttgatt tcaaactcga gtctcatgca tttgcttcta aaacagaata
     1021 cttcaatgct gagctggtta aggatatatg cgcaatggct aacaatggaa atgttagtag
     1081 ttatttaatt attggtgttt cagatgatgt taagagtttt aagagcgttc aaaatcaaaa
     1141 actgactgat gaaaatgttc aaagtctatg caaaactgct atatatcctc ctccaaaaat
     1201 tagatttctt aagaagagat ggtctgaaaa tgttttacat gaacataaag acaaagattt
     1261 tgtaattatc caaattggac ctcacccaaa acaagtgttc cgtttagcaa aagattttat
     1321 cgattacaaa gaaaaaaatt tgtttcaaaa gaaatgaagt ttgggttcga cggggtacaa
     1381 catcggattt agcaacccca gaagaaattc tgaaattgtt ttcgggcgat ccaataaaca
     1441 tttcggaaga agcagaagca aggaaacaag agcgttttat tttttcgaac ctttcggttt
     1501 ctgaaaaatg ttctgcgatc tttggaaaaa tggaaaattt tgttggaaat gatttaggct
     1561 ataaaaagtc gacctggcat aagtctccat tttcatttca agtaaactgg aagcagatag
     1621 ggtcaaccct tttttttatt aactgttttt catgtgataa aagttttaca aaacaaagcc
     1681 ttgattggtt ttattctcgt aagttttttt cttttgacta tattgcgaaa aaagatttac
     1741 ccgctgagtt taatgagtat ctagaaacga tcaagttgat taggaggatt accttgtggg
     1801 catttttgga tggagtaagc caaaataaag ttatggcaaa ctttccttct atgaggaagt
     1861 ctgaaattca gcatcattat tatcatggcg aatggcaaga taataatgat aaaaataaaa
     1921 agacaatcta tttaccttca agtaatgaaa tggttttaat tgataaaatt aaatctttac
     1981 cagattttac tgaaacactt gaagacgtaa taaaagaact ggaaaaagat aataaggcta
     2041 cagtttttgc tcctgaaatc aagaaagcga tttggtgagt tgatggatac gttgttaccg
     2101 tccgtgttgg atagggcgtt taagggagag ctgtagcccc ctctgtcctt tggacatctc
     2161 ccccaaatgc gacgatagtg ctgtcggatt tgggggagaa ctggaaatgg agagtatgaa
     2221 tgacacaaaa gacaaagata tcactcgacg agcagcggcg cattgtcgct tatctggatg
     2281 ggttgcaggc gaaggtcaat gcgttgc
//
LOCUS       DAOQJE010000642         2305 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_35197_length_2305_cov_5.169778, whole genome shotgun sequence.
ACCESSION   DAOQJE010000642 DAOQJE010000000
VERSION     DAOQJE010000642.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2305)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2305)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2305
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35197_length_2305_cov_5.169778"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1129)
                     /locus_tag="PLT08_14615"
     CDS             complement(<1..1129)
                     /locus_tag="PLT08_14615"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNE05756.1"
                     /translation="MSEPLLKVESLSKNFDRLPVLRDVSFQVDKGEVIGLVGRQGAGK
                     STLLHLLGGAIPASSGTINYLGKSRRFFNRLEAQSLGIETVYQTSSLINYFDFSSQIF
                     LDRESDIKNTRQTSGLVEQFNVTQNMLLGREIMKVPSLGVLDHDKMVDIAQELLAEFN
                     LPKYLAVEKVRNLTDEQRQIVLLLRMLYHPCQLLLLDDILPILSFDRQNILLERIRKL
                     ADQGTSVIISSDDLKHLFTVTDRIIVLYEGRLVADRRTSESTPREIVELMVGSAAHGQ
                     VSPLIWALESYHTAQQQTEDLRRTQASLKQSLDARTSLNRQLVERLSNQLVALDQLNA
                     ALQATQRRLITEREDERKALARELHDQIIQDMLGLIYHLEEI"
     gene            1195..2244
                     /locus_tag="PLT08_14620"
     CDS             1195..2244
                     /locus_tag="PLT08_14620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HNE05757.1"
                     /translation="MSLVRVLLADDHTVVRAGLRNALEVLPNLEIVGEVGDGIELMNA
                     ISQSPIDLLVMDASMPDFEPISAVQQIKEKYPSIKILVVSAYNDESYVVGLLKAGASG
                     YHLKDQPLSDLQLAAQRVLSGQRWISSNLIDRLLTRQAPTPSTNLPYLTRRQRELLRL
                     ISQGADNRNIAQALDLSIKTVENHLTALYRVLGVDSRLKALNYILRHPELLASTGQEM
                     AETNTPVKGQEFTFLIVDDNPRYRQQLGRLVGKIYPSVRLYEADTTAEALSISKQVQP
                     QLAFVDVVLDDEDGIQCARKLRTVSPQTRIVVISAYPDKEFRRQALSAGVEVFLDKKD
                     LDTASVQQVLEDALR"
BASE COUNT          590 a          511 c          548 g          656 t
ORIGIN      
        1 cgatctcttc caaatgatag atcaaaccca gcatgtcttg aatgatctgg tcatgcaatt
       61 cgcgggctag agctttgcgt tcatcttcgc gttcggtgat gagacgcctt tgcgtggctt
      121 gtaaggctgc gttcaactgg tcaagcgcta cgagctgatt gctcaaccgc tctacaagtt
      181 ggcgatttaa cgatgtgcgt gcatctaggc tttgctttag agaagcttgt gttcggcgta
      241 aatcttctgt ttgctgttgg gcagtgtgat aactttccaa tgcccaaatt aacggagaga
      301 cctgaccgtg agcggctgag cccaccatca gctccacgat ctcacgcggt gtggactcgg
      361 atgtgcgtcg atctgcaacg aggcgacctt cgtatagcac aataattcgg tcagtgactg
      421 taaatagatg ctttaggtca tcactgctaa tgatgactga agtcccttga tctgccagtt
      481 ttcgaatgcg ttctaataaa atgttctggc ggtcaaaact taatatgggc aaaatatcat
      541 ccagcagtaa tagctgacat ggatgataaa gcatgcgtaa cagaagaaca atctgacgtt
      601 gttcgtctgt cagattgcgt actttttcca cggcaagata tttgggtaaa ttgaactctg
      661 ctaaaagttc ttgggcaata tccaccattt tgtcgtgatc caaaacccca agcgaaggga
      721 ccttcatgat ctcacgccct agaagcatat tttgggtcac gttgaattgt tccaccaaac
      781 ctgatgtctg acgggtattt ttgatgtcgg attctctgtc taaaaagatt tgcgaggaga
      841 aatcaaaata gttgatcaag cttgatgttt gatagaccgt ttcaattccg aggctttgcg
      901 cttccagccg attgaagaac cgcctactct tgccaagata gttaatcgtc ccagatgagg
      961 cgggaatggc tccaccaagt aaatggagca aggtcgattt ccccgctcct tgacgtccca
     1021 ccagcccgat gacttcacct ttatccactt gaaacgaaac atcccgcaac acgggcaaac
     1081 ggtcgaagtt tttggaaaga ctctctactt tcaacaaggg ttcagacata atttatttta
     1141 acctgattct gtgggcttct tttcagtttt tgttacttac ggcataatag atgcatgtct
     1201 ctcgtacgag ttcttttagc tgatgatcat accgttgttc gggctggttt gcgaaatgcc
     1261 ctggaagttc ttcctaatct tgaaattgtg ggtgaagttg gcgatggcat tgaattgatg
     1321 aatgccattt cacagagccc tatcgattta ttggtaatgg atgccagcat gccagatttt
     1381 gaaccgatct ctgcggttca gcagatcaag gaaaaatatc cgtcgatcaa aattcttgtg
     1441 gttagcgctt ataacgatga atcctatgtt gtgggacttt tgaaagcagg cgcaagcggt
     1501 taccacttaa aggatcagcc attatcagat ttgcagttgg cggctcaacg cgtattaagt
     1561 ggacagcgtt ggatatcgag caatttgatt gaccgtttgt tgacgcgtca ggcgccgacg
     1621 ccttctacca atttaccgta tctcacccgc cgccaacgtg aattgttgcg gctgatttct
     1681 caaggtgccg ataatcgcaa tattgcccaa gctttagact taagtatcaa gacggttgaa
     1741 aatcatctca cggctttgta tcgcgttttg ggcgtagata gtcgcttgaa agcattgaac
     1801 tacatcttgc gccaccctga attattggca tccaccgggc aggaaatggc agagacaaat
     1861 acaccggtca aggggcaaga atttacgttt ctgatcgtgg atgacaatcc gcgctatcgt
     1921 cagcagcttg ggcgtttggt ggggaagatt tacccatctg tccggttgta tgaggcggac
     1981 actacggctg aagcgttatc aattagcaaa caggtccaac cgcaattggc ttttgtcgat
     2041 gtggttttag atgacgaaga tggtattcaa tgtgctcgta aacttcgcac agtttcgccc
     2101 caaactcgaa ttgtagtaat tagtgcatac cctgataaag agtttcgccg acaggcactg
     2161 tctgctggcg tggaagtgtt tctcgataaa aaagacctcg acacagcttc tgtacaacaa
     2221 gtccttgaag atgcattgcg ctagtacgtc acgatttttg aatatgcggg acgtttgaaa
     2281 gaatatcatc cactacaata gcgac
//
LOCUS       DAOQJE010000643         2291 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_35609_length_2291_cov_4.288462, whole genome shotgun sequence.
ACCESSION   DAOQJE010000643 DAOQJE010000000
VERSION     DAOQJE010000643.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2291)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2291)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2291
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35609_length_2291_cov_4.288462"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..406
                     /locus_tag="PLT08_14625"
     CDS             <1..406
                     /locus_tag="PLT08_14625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE05758.1"
                     /translation="SAPSAFHADFFKLIRLRYEIEAPERVWNLGASLSSMTIYRQSEN
                     TLVINASNGYISGFDTVFRGNAHPMFKGQTVNLNGLQVTVQAVTPDGRPAMVRFEFAE
                     PLQSAEYQWLIWKSNRLIEWNVPEIGQSVHVP"
     gene            complement(411..1316)
                     /locus_tag="PLT08_14630"
     CDS             complement(411..1316)
                     /locus_tag="PLT08_14630"
                     /inference="COORDINATES: protein motif:HMM:NF015722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HNE05759.1"
                     /translation="MSKARKLIPYFIVFDILVLIAVIVALNFDVVLRLNNAYKAGTLT
                     EQKIRYEIAAYDLNDWRRVEPIDQKTSPRDGMVQVYVPEGEFIMGKPGEPDFDSPEHL
                     VYVNSFWIDRVEISNQLYAACVEAGGCSQPFIDENPFYGRWVYRNLPVTFVNWYQATE
                     YCEWAGRRLPTEAEWEKAARGTDQRPYPWGEADATPRLANYAESLIGEPVSVYRYPSG
                     ASPYGALNMVGNVREWLADWFSTDYYLSSPSNNPTGPETGIERSMRSGAYDADTNEIY
                     TTSRYKHEPQSAGLSRGFRCAETAK"
     gene            complement(1346..>2291)
                     /locus_tag="PLT08_14635"
     CDS             complement(1346..>2291)
                     /locus_tag="PLT08_14635"
                     /inference="COORDINATES: protein motif:HMM:NF012356.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HNE05760.1"
                     /translation="DPSGYFQKAQYSQDQPESFELAKELRQVTNEFGERLTVGEITAK
                     RNIVRKFAGEEKNDGLTLAFDFEMLEFAFTAEYFRKLIGNIETHFHPPFMPVYVFSNL
                     DKRRSIHRLGDDVRKAKLLHMLQLTVRGVPCMYYGEEIGISNLKLPFNTALDPIPHKY
                     TFAPRFIFDMLDITVNRDEVRTPMQWDTTKNAGFSSAEKTWLPVHPNHSLVNVESESK
                     DEHSLLNTIKSLMKFRNENSAIKEGDFEFIDGLPAGVLGYKRSLNGQSFLVLLNFDET
                     AKDVRLRFQNAAFRLTVKDEAQGDIAHLNSFGGLILFV"
BASE COUNT          590 a          556 c          531 g          614 t
ORIGIN      
        1 ttctgctccc agcgccttcc acgcagactt tttcaaactc atccgcttgc gttatgagat
       61 agaggcacct gaacgcgtat ggaatttggg agcaagtctc tcgtctatga cgatttatcg
      121 ccaaagtgaa aacaccttag tgatcaacgc ttctaatggt tatatttcag ggtttgatac
      181 tgttttccgt ggcaatgctc atcctatgtt taaggggcag actgtaaact taaacggctt
      241 gcaagtcacc gtgcaagccg tcacaccaga tggacgccct gcaatggtaa ggtttgaatt
      301 tgccgagcca ttgcagagcg ctgagtatca gtggttgatc tggaaatcaa atcgccttat
      361 cgaatggaat gtccctgaga tagggcaatc tgttcacgtt ccgtgaaata ctattttgct
      421 gtctccgcac agcggaaccc gcggcttaat cctgctgact gtggctcgtg tttatagcgt
      481 gatgtagtgt aaatttcatt agtgtccgca tcataagcgc cactgcgcat tgaccgttca
      541 atgccagtct ctggaccggt tgggttattt gatggcgaac tgagatagta atcagtgctg
      601 aaccaatctg ccaaccattc gcgcacgttg ccaaccatgt tgagcgcacc ataagggctg
      661 gctccgcttg ggtaacgata cacagaaacg ggttcaccga tcaaagactc ggcgtagttc
      721 gcgaggcgcg gagttgcatc cgcttcgccc cacggatatg ggcgttgatc ggttccacgc
      781 gcagcttttt cccattccgc ttcagtaggc aaacggcgac cagcccattc acagtattca
      841 gtggcttggt accagttcac aaaagtaacc ggcaaattgc gatagaccca tctgccataa
      901 aatggatttt catcaataaa cggttgcgaa caaccaccag cttccacaca agcagcatac
      961 agttggttag atatttctac gcggtcaatc caaaatgaat tcacatacac gagatgctca
     1021 ggcgaatcaa aatctggttc gccgggcttg cccataataa attcaccttc aggcacatac
     1081 acttgcacca taccgtcacg aggcgaggtc ttttggtcaa taggctcaac acgccgccaa
     1141 tcgttcaagt catatgcagc aatctcatag cggatctttt gttctgtcag cgtgccggct
     1201 ttgtatgcgt tattcagacg cagcactaca tcaaaattga gtgcaacaat tactgcaatc
     1261 aacacaagga tatcaaaaac aatgaagtaa ggtatcagtt ttcgggcttt agacatgggc
     1321 gacattatac ctagcacaga tgcctttata caaaaagaat cagcccgccg aaactgttca
     1381 agtgcgcaat gtcgccctgc gcttcatcct taacggtgag cctgaatgcg gcattctgga
     1441 accttaaacg cacatccttt gccgtttcgt cgaagttcaa gagcacgagg aaagattgtc
     1501 cgttcagaga acgtttgtat ccgagtacgc ctgctggcaa tccgtcaatg aattcaaaat
     1561 ctccttcttt gatggctgaa ttctcattcc tgaatttcat caaagatttg atggtgttca
     1621 ataaggagtg ttcatcttta ctttcacttt ccacattcac aaggctgtga tttgggtgaa
     1681 caggcagcca cgttttttca gcggacgaaa accctgcgtt ctttgtggta tcccattgca
     1741 tgggtgtacg gacctcgtcg cggttcacgg taatatcgag catgtcgaag ataaagcgtg
     1801 gggcaaaagt atatttatgc ggaatgggat caagcgccgt gttgaatggt aatttcaaat
     1861 tgcttatacc gatctcttcg ccataataca tgcaaggcac accgcgcaca gttagttgca
     1921 acatgtgaag cagcttcgcc ttgcgcacgt catcgccgag gcggtgaatg ctacggcgct
     1981 tgtcgagatt gctaaataca tagaccggca taaagggcgg atgaaaatgc gtttcaatat
     2041 tgccaatcaa cttgcggaag tattcggctg tgaaggcgaa ttcgagcatc tcaaaatcga
     2101 aggcaagggt cagcccatca ttcttttctt ctcccgcaaa cttgcgcacg atattccgtt
     2161 ttgccgtaat ttcaccgacc gtgaggcgtt caccaaattc gttggtaact tgtctcagtt
     2221 cctttgcaag ttcaaagctt tcaggttgat cttgtgagta ttgtgctttc tgaaaatacc
     2281 cagaggggtc a
//
LOCUS       DAOQJE010000644         2289 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_35668_length_2289_cov_3.827216, whole genome shotgun sequence.
ACCESSION   DAOQJE010000644 DAOQJE010000000
VERSION     DAOQJE010000644.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2289)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2289)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2289
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35668_length_2289_cov_3.827216"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..349)
                     /locus_tag="PLT08_14640"
     CDS             complement(<1..349)
                     /locus_tag="PLT08_14640"
                     /inference="COORDINATES: protein motif:HMM:NF012263.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE05761.1"
                     /translation="MTLTNPYAQRKTLRAFEKVWTKLEGFVNRFTKSDFNPLYHLGTL
                     TIFMLLILIATGAYLTMIYRPGLDVAYRTVEKIDSNWFGSLMRSVHRYASDGMIVLIV
                     LHLLKMLFSDRFWG"
     gene            complement(443..>2289)
                     /locus_tag="PLT08_14645"
     CDS             complement(443..>2289)
                     /locus_tag="PLT08_14645"
                     /inference="COORDINATES: protein motif:HMM:TIGR01525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HNE05762.1"
                     /translation="SPETAWLVHFFQIMMLVTSIPVLILLGLPILRAGFASLLRGQPN
                     IHTLIMIGTFSAYALSVRNFMLGHGGLYFDTATMLIFLVSIGRWLEMQAHKSSNKAVA
                     RLLEQIPNEATVVAGDGDKTVKVSELKAGMRVRVRPGERFPVDGLIAIGEGDVDESLL
                     TGEPKPVTHREGDKVQAGTVSLDGSFEVIATAVGESTTAGQIGRLLHEALWARSPLER
                     MADKLSAWMTPLALALATIAFLIWYSISGLETGLMVALSVLLIACPCALGLATPLTLW
                     LSLERAAESGAILRSTGALERLAKVERIFFDKTGTLSQLPMKVLEIKEERGKKKEFLE
                     VVASVENESEHPLAKAIVEYAKSQNVEFIKPTSFKVLPALGVIGQLPITNYQSSILIG
                     SARLMSTEGLNMPAEISQQAEAWKEAGHIVVYAGWDGQVMGILALGEKIRAEAKEVVN
                     QLQSRGLELGVLTGDEASAGERWQKALGIPVSAALSPDEKMKRLAENSAMVGDGINDG
                     PALAAATVGLAMNHGTDVARTAADIVLVRDDLRVVPWLVDLSRESMKRVRQNLGWAVI
                     YNLIGVALAMTGLLQPIFSAFAMVASSIFVTTNAMKMNQFPLLEEKEN"
BASE COUNT          542 a          656 c          567 g          524 t
ORIGIN      
        1 gaccccaaaa gcggtcgctg aataacattt tgagcaaatg caaaacgatg agcacaatca
       61 tgccatctga tgcgtaacga tggacactgc gcatgagcga gccgaaccaa ttggaatcta
      121 ttttctcgac agttcgataa gcaacatcca aaccaggacg atagatcatg gtcaggtatg
      181 cgcctgttgc gatgagaatg agcaacatga agatggtcag cgtgccgaga tggtacaacg
      241 ggttgaagtc tgattttgtg aatcgattga caaagccttc gagttttgtc caaacttttt
      301 caaaggcgcg gagagtcttg cgttgagcgt aggggttggt aagtgtcatg tgtcaggttc
      361 caagtttcaa gttttgaact gcaaagaccg cgaaggtcgc taagaaagtc ttttttcttc
      421 gcgctctttg cgtacttcgc ggttaatttt ctttttcttc caacagcgga aattggttca
      481 tcttcatcgc attggtcgtg acgaagatac tgcttgctac catcgcaaat gccgagaaga
      541 tgggttgcag cagacccgtc atggcgagtg caactccgat caggttgtag atcactgccc
      601 agccgagatt ttggcgcacg cgtttcatcg actcgcgcga gagatccacc agccagggga
      661 cgacgcgtag atcatcacgc acgagcacaa tgtcggcggc ggtacgcgcc acgtccgtgc
      721 cgtgattcat cgccagccca acggttgcgg cggcgagcgc gggaccgtca ttaatgccat
      781 cgccaaccat cgcggaattt tctgccagcc gcttcatttt ttcatcgggg ctgagcgccg
      841 cgctgacagg aattcccaac gccttctgcc agcgttcgcc cgcactggct tcatcacccg
      901 tcagcacgcc gagttccagc ccgcgcgatt gcaactgatt cacaacctct ttcgcttcag
      961 cacggatttt ttcaccgagc gcgagaatac ccatcacctg accatcccag cccgcataga
     1021 ccacgatatg ccccgcctct ttccacgcct cagcctgttg gctgatttcc gcaggcatat
     1081 tcaagccttc ggtagacatg aggcgggcgg aaccgatgag gattgatgat tggtaattag
     1141 taattggtaa ttgtccgatg acgccgaggg cggggaggac tttgaaggat gtgggtttga
     1201 tgaattcaac attttgtgac ttggcatatt ccacaattgc tttcgccagc ggatgttctg
     1261 attcattttc aaccgaagcg accacttcca aaaattcttt cttctttcct ctttcttctt
     1321 taatctccag caccttcatc ggcaattgac tgagcgtgcc cgtcttatcg aagaagattc
     1381 gctccacttt ggcgagccgt tccaatgcgc cagtgctgcg taaaatcgcg ccagattcgg
     1441 cggcacgttc cagcgaaagc cacaaggtca gcggagttgc cagccccagc gcacacggac
     1501 aggcgatcaa caacacggag agcgcaacca tcaaccctgt ttccagcccc gaaatggaat
     1561 accaaattag aaaagcaatc gtcgccaacg ccagcgccaa cggagtcatc cacgcggaga
     1621 gtttatctgc catgcgttcg agcggagaac gcgcccacaa cgcttcatgc aacagccgcc
     1681 caatctgccc cgccgttgtc gattctccga cagcggttgc gatgacttca aaacttccat
     1741 cgaggctgac cgtgcctgcc tgtaccttat cgccttcgcg atgagtcacg ggtttgggtt
     1801 cccccgtcaa cagcgattca tcaacatccc cttcgccgat agcgatcagt ccatccacag
     1861 ggaatctctc gccagggcga acccgcacgc gcatccctgc tttcagttcg gagactttca
     1921 cggttttatc accgtcacca gcaaccaccg tcgcttcgtt agggatttgc tccaacagcc
     1981 gcgccaccgc tttgttgctg gatttatgcg cctgcatttc aagccagcgc ccgatcgaaa
     2041 ccaaaaagat gagcatggtc gccgtgtcaa aatacaaacc gccatgcccc agcataaaat
     2101 tccgcaccga caatgcatac gccgaaaacg tgccgatcat aattaacgtg tgaatgttgg
     2161 gctgtccgcg cagaaggctt gcgaaccctg cgcgcaagat cggcagaccg agcaagatca
     2221 aaacagggat gcttgtcacc agcatcatga tctggaaaaa atgcacgagc cacgccgtct
     2281 ctgggcttg
//
LOCUS       DAOQJE010000645         2287 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_35718_length_2287_cov_4.330645, whole genome shotgun sequence.
ACCESSION   DAOQJE010000645 DAOQJE010000000
VERSION     DAOQJE010000645.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2287)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2287)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2287
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35718_length_2287_cov_4.330645"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1864
                     /gene="polA"
                     /locus_tag="PLT08_14650"
     CDS             <1..1864
                     /gene="polA"
                     /locus_tag="PLT08_14650"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF004397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HNE05763.1"
                     /translation="FAEEAQPVAVAIKPASIEVVGVDSAEKLKSLANELEKAKVISFD
                     TETTGTEEMTAELVGISLAVKEGQGYYIPVGHVAGDNLPIQTVIDAITPAMTNAKIGK
                     VAHNAKYDYIVLARYGLTVSPLTFDTMLAEFIVDPASRHLGLKNLSEQRLGEEMTHIE
                     ELIGKGKKQISMAEVAIEAVANYAAADAETTLRLMAIEEKELKRVEGQKILDEIDMPL
                     TAVLAEMEMTGALIDTKFFGEMSKELAARLNEIEKEIYGHVGKTFNINSPQQLSDVLF
                     NHLRLEPPDKGRKTSTGFYSTSADVLDSMRGQHPVLDFILEHRELSKLKSTYVDALPA
                     AVDANTGRVHTSYSQIGAVTGRLSSNNPNLQNIPIRTETGRKVRNGFIADKGNLLLSV
                     DYSQIELRIVAHMAQDEAMLAAFRAGEDIHATTAAAVYDIPLEQVNKDMRRHAKAINF
                     GLIYGMSAFGLMHSTGLTLAESEDFVKAYFKKFPGIKKYLDGIRRDAAEIGYVETLLG
                     RRRYFPALQTKTNVQLKNREEREAINAPIQGTAADIMKIAMLNIPPALQKAGLKAKML
                     LQVHDELVLEVPKKEIEQTAKLVQETMANAYPMSIPLETEARAGVSWGEMTVLE"
     gene            2036..>2287
                     /locus_tag="PLT08_14655"
     CDS             2036..>2287
                     /locus_tag="PLT08_14655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE05764.1"
                     /translation="MRPQKNTVAPRVTVNVSSEMANLRKIQGALNGFWQTSDLSYGFF
                     LLNSDTTFSNKERKTVDLLGNIDSDVWYLNNQGRIKYDNN"
BASE COUNT          609 a          514 c          659 g          505 t
ORIGIN      
        1 gttcgcagaa gaggcgcaac ccgtggcggt ggcgatcaaa ccggcgagca ttgaggttgt
       61 gggtgtggat agcgccgaaa aattgaaaag cctcgccaac gagttagaaa aagccaaggt
      121 gatttcgttc gataccgaaa cgacgggcac cgaagaaatg acggctgaat tggttggcat
      181 ttcactggct gtgaaagaag ggcaaggcta ttacattcca gttggacatg tggctggcga
      241 taacctgccg attcaaaccg tgatcgatgc gatcaccccg gcgatgacga atgcgaagat
      301 cgggaaggtg gcgcataacg ccaagtacga ttacattgtg ctggctcgct atgggctgac
      361 cgtctcgccg ttgacctttg atacgatgct ggctgagttc attgtggacc ctgcttctcg
      421 tcatttggga ttgaaaaatc tctctgagca gcggcttggc gaagagatga ctcatattga
      481 agagctgatc ggcaagggca aaaagcaaat tagcatggca gaggtggcga ttgaagcggt
      541 tgccaattat gcggctgccg atgcggagac gaccctgcgt ttgatggcaa ttgaagagaa
      601 agaactcaaa cgcgtggaag ggcaaaagat tttggatgag atcgacatgc cgctgacggc
      661 tgtgctggct gaaatggaaa tgaccggcgc attgatcgat accaagttct tcggcgagat
      721 gtctaaagaa ttggcagcgc gtttgaacga gatcgaaaag gaaatttacg gtcacgttgg
      781 caagaccttc aacatcaact cgccacaaca gctttcggat gtgctcttca atcacttgcg
      841 cctcgaacca cccgataagg gacgcaaaac atccacgggc ttttactcca cctcagccga
      901 tgtgctcgac tcgatgcgcg gacaacaccc ggtgttggac tttatccttg agcatcgcga
      961 actctcgaag ctgaaatcca cctatgtgga tgcgttgccc gccgccgtgg atgcgaacac
     1021 gggcagggtg cacacctcgt acagccagat cggcgcggtg acggggcggc tctcttccaa
     1081 caacccgaac ttgcagaaca tccccatccg cactgagacg gggcgaaaag tgcgcaatgg
     1141 cttcattgcc gataaaggca atttgctact ctcggtggac tactcgcaga tcgaactgcg
     1201 cattgtggcg cacatggcgc aagatgaagc gatgttggcg gcgttccgcg cgggcgaaga
     1261 tattcacgcc acgacggcgg cggcggtgta cgatattccg ctggagcagg tcaacaagga
     1321 tatgcgccgg catgccaagg cgatcaactt cgggttgatc tatggcatgt ctgcttttgg
     1381 gttgatgcat tcgacggggc tgacgctggc tgagtctgaa gattttgtga aagcgtattt
     1441 caagaaattc ccgggcatta agaaatattt ggatggcatt cggcgcgacg cggcggagat
     1501 cgggtatgtg gaaaccctgc ttgggcggag gcgctacttc ccagccttgc agaccaagac
     1561 caacgtccag ttaaagaacc gcgaagaacg agaagccatc aacgccccca ttcaaggcac
     1621 ggcggcagac atcatgaaga tcgcgatgtt gaacatccca ccggctttgc agaaggcggg
     1681 cttgaaagcc aagatgctgt tgcaagtgca tgatgagttg gtgttggaag tgccgaagaa
     1741 agagatcgag cagaccgcca agcttgtgca agagacgatg gcgaacgcct acccgatgag
     1801 cattccgttg gagaccgaag cgcgtgccgg tgtgagctgg ggcgagatga cggtgttgga
     1861 ataataggaa ggggcaagcc ggttttcttg cccctttttg gcgggacttg tataatgacg
     1921 ttatgcaaaa taaaggggat tatgcagcat gatgctgtat aaaaccccaa attgggagta
     1981 catacagcat agtgctgtat aaaacctagt taggcggtag aagaaggaaa atccaatgag
     2041 accacaaaaa aatactgttg cgcctcgagt tactgtcaat gtgtctagcg aaatggctaa
     2101 tttgcgtaaa attcaaggag cactcaatgg tttttggcaa actagcgatt tatcttatgg
     2161 cttctttttg ttgaatagcg atactacttt ctctaacaaa gaaagaaaaa ctgttgattt
     2221 gttggggaac attgattcag atgtatggta cttgaataat caaggcagaa ttaagtatga
     2281 taacaac
//
LOCUS       DAOQJE010000646         2282 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_35870_length_2282_cov_6.916480, whole genome shotgun sequence.
ACCESSION   DAOQJE010000646 DAOQJE010000000
VERSION     DAOQJE010000646.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2282)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2282)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2282
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35870_length_2282_cov_6.916480"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..854)
                     /locus_tag="PLT08_14660"
     CDS             complement(<1..854)
                     /locus_tag="PLT08_14660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE05765.1"
                     /translation="MKKSTLTRILLLLALLIVPTASIHAQTPNNDDVVLFGQNYTLEE
                     GETLNGSLAVFGGNIDIKEGAEVNGSIAIFGGNITFAKDTTINGDVAVFGGNVSVEGE
                     IAGDMVLFGGQALLESKSKVRNIATFGGQVTQQEGSIVTGEITNNAPPEVDTPPSLPE
                     EPTVDIQVNPLWNVLGKFGQALLIAVIGMLLTLFLRPQLDRTADTITRQPLMAGGYGL
                     IVFFALPIALVIMVITLILIPVAAIVVLLIPLAWLFGIVALGQEVGERFSKAINQVWA
                     PVLSTGLGT"
     gene            complement(851..1381)
                     /locus_tag="PLT08_14665"
     CDS             complement(851..1381)
                     /locus_tag="PLT08_14665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-HC2 domain-containing protein"
                     /protein_id="HNE05766.1"
                     /translation="MNHQPFETWLLDDKVLTPTEKRELDSHLRTCKNCTALAETGLAL
                     RSSRVATPAPGFAMRFQQKLAAQKLAEQRRRLWGMIVLIVSGVGLLGWFLAPVLMSIT
                     TSPVGFLLSAAGLFAFLFTSIQAFGEVLTVMLRMLPAFLPPYMWMVLFSGAAGIGLLW
                     AVSIWRLVRRPQGVFA"
     gene            complement(1362..1970)
                     /locus_tag="PLT08_14670"
     CDS             complement(1362..1970)
                     /locus_tag="PLT08_14670"
                     /inference="COORDINATES: protein motif:HMM:NF019265.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HNE05767.1"
                     /translation="MNEEQTWVLQAQQGNDEAFTKLVETYQTPVFNLCYRMLGEPELA
                     EDAAQETFLRAYQHLHRYDQKRPFPTWLLSIAAHYCIDRLRRRKFSMFSMDTEDEEGN
                     SFEIPDVDAPNPEGEAITGQTNERVHAMLKDLDATDRAAIIMRYWYDYSEKEIAESLN
                     LTVSAVKSRLHRARKELAGLWAEQEDDLLAEMERRHHESPAF"
     gene            complement(2067..>2282)
                     /locus_tag="PLT08_14675"
     CDS             complement(2067..>2282)
                     /locus_tag="PLT08_14675"
                     /inference="COORDINATES: protein motif:HMM:NF019522.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibE/F family protein"
                     /protein_id="HNE05768.1"
                     /translation="MFSLGHGDYGYIVNFSFVAEEIVRTLVGSLGLIAAVPLTTVIAI
                     FFAQRADSLGKWEQVLGPEGDGHGHVH"
BASE COUNT          565 a          590 c          599 g          528 t
ORIGIN      
        1 gttccgagac cggtggaaag aaccggcgcc caaacttggt tgatggcttt gctaaagcgt
       61 tcaccaactt cctgacccaa cgccacgatg ccgaatagcc atgccaaggg gataagcaag
      121 acgacaattg ccgcaacggg gatcaagatt aacgtaatga ccataatgac caatgcaatc
      181 ggcagggcaa agaatacaat caagccgtag ccgccagcca ttaagggttg acgtgtgatg
      241 gtgtctgcgg tgcggtctaa ttgcgggcgc aagaagagcg tgagcaacat gccgatgacg
      301 gcgatcaata aagcctgacc gaattttcca agcacattcc ataacgggtt gacctgtata
      361 tctactgtcg gttcttctgg caggctaggc ggagtatcca cttcaggcgg tgcattgttg
      421 gtgatctcac cagtgacgat agagccctct tgttgagtga cttgtccgcc aaaggtggcg
      481 atgttgcgaa cctttgactt tgactcaagc aaagcttgcc cgccaaagag caccatatcg
      541 cctgcgatct caccttcaac gctgacattt ccaccaaaga cggcgacatc accgttgatg
      601 gtggtatctt tggcgaaggt aatgtttcca ccaaagatgg cgatcgaccc gttgacctct
      661 gccccttctt taatatcaat attgccgcca aaaacagcaa ggctgccatt cagcgtttca
      721 ccctcttcca gcgtgtagtt ttgaccgaat agaacgacat catcattatt cggggtctgc
      781 gcgtggatgc ttgctgtagg cacgatcaaa agtgccagca gaagcaggat tcgggttagc
      841 gttgattttt tcatgcaaaa actccttgag gacggcgaac gaggcgccag atggatactg
      901 cccataacaa cccaataccc gctgctccag agaagagaac catccacata tagggaggga
      961 gaaaagcagg aagcatgcgc agcattacag tgagcacttc accgaatgct tgtatggatg
     1021 tgaatagaaa tgcaaataac ccggcagcag agagcaaaaa gcccaccggc gaggttgtga
     1081 tcgacatcaa aacgggagca agaaaccatc cgagtaaccc aactccgctg acgatgagaa
     1141 cgatcatgcc ccatagacgg cggcgttgtt ctgccagctt ttgcgccgcc agtttttgct
     1201 ggaagcgcat ggcaaaccca ggtgcagggg ttgctacccg agaagagcgt aaggcaagcc
     1261 cggtctctgc cagtgcggtg caattcttgc aagtgcgcag atgagagtcg agttcacgtt
     1321 tctctgtagg ggttaggact ttatcgtcca acagccaggt ttcaaaaggc tggtgattca
     1381 tggtgtctcc tttccatttc ggcgagcagg tcatcttctt gttcagccca caagccagcc
     1441 agttcctttc gtgctcggtg caggcggctt ttcaccgcac tcaccgtcaa attcaaggac
     1501 tcggcaattt ccttttctga atagtcgtac caatatctca taatgattgc cgcacggtcg
     1561 gtggcatcca agtctttgag catggcatgg actcgttcat tggtctgccc ggtgatggct
     1621 tccccttcag ggttgggcgc atccacatcg ggaatttcga acgagttacc ttcttcgtct
     1681 tcggtatcca tcgagaacat tgagaacttg cggcggcgaa gacggtcaat gcagtaatgc
     1741 gccgcgatcg agagcaacca agtcgggaag gggcgtttct ggtcatacct gtgcaaatgt
     1801 tggtaggcgc gcaggaaggt ttcctgagca gcatcttctg cgagctctgg ttcacccaac
     1861 atgcggtaac acaggttgaa aacaggggtt tggtaggttt caacgagttt ggtaaacgcc
     1921 tcgtcattac cttgttgcgc ctggagaacc caggtctgtt cttcgttcac gtttctcctc
     1981 gggaaggctt tctaccttct cttacgtgcg tactggagga aaagtttcac aaatttgact
     2041 gtacaagacg caggatgggt cattttctag tggacatgcc catgcccgtc tccttcggga
     2101 ccgagaacct gttcccactt cccgagcgaa tctgcccttt gagcgaagaa aatggcaatg
     2161 acagtggtta gcggcaccgc ggcaatcagc cccagcgagc cgaccaaggt ccgcacgatt
     2221 tcttcagcca caaacgagaa gttgacgatg tagccataat cgccatgtcc aagcgagaac
     2281 at
//
LOCUS       DAOQJE010000647         2279 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_35970_length_2279_cov_6.554406, whole genome shotgun sequence.
ACCESSION   DAOQJE010000647 DAOQJE010000000
VERSION     DAOQJE010000647.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2279)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2279)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2279
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35970_length_2279_cov_6.554406"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            25..1299
                     /gene="hutI"
                     /locus_tag="PLT08_14680"
     CDS             25..1299
                     /gene="hutI"
                     /locus_tag="PLT08_14680"
                     /EC_number="3.5.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050480 - imidazolonepropionase activity
                     [Evidence IEA];
                     GO_process: GO:0006548 - histidine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazolonepropionase"
                     /protein_id="HNE05769.1"
                     /translation="MLIHSASQVLTLSGGPQRGKMLGTLGIIPNGAVVVRDEKIIAVG
                     TTDELRNAYPDEPTLDAGRCVLMPGFVDPHTHLVWAGDRADEFEKKMAGTPYLDILAA
                     GGGILSTVRATRTASLETLMAETRPRMLRMFRNGTTTAEAKTGYGLQTSTELRLLKAL
                     LALNDESPMDLVFTFLGAHAIAPEFKDNPQGYTDEVINTMLPMLKSWWETHAPRHTLP
                     FVDVFCETKAFTLEQARQILTQAMALGFPLKIHADEFDNLGGAALAAELKAASADHLV
                     VTSDADIEALGKSDTVAVALPCTPFGLAEAHYTPAKKLIEADALLAVATDCNPGTSWN
                     ESMQFAIALACRYMKLTPAQAIAAATINAAHAIRKADMIGSIEVGKQADMLILSVNDY
                     RQVGYRYGTNLVKQIIKRGQVYSVDVGYYRTA"
     gene            1305..>2279
                     /locus_tag="PLT08_14685"
     CDS             1305..>2279
                     /locus_tag="PLT08_14685"
                     /inference="COORDINATES: protein motif:HMM:NF028237.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase N-terminal 7TM
                     domain-containing protein"
                     /protein_id="HNE05770.1"
                     /translation="MNNSWSIFAGILIFNAITALAIAFILTRRPQKNGIKPLTLMFIS
                     LAFWSFGYAMITLSPQLKDKIFWLKFENFGIQTTALFWFLFAMMYTKMDSWMDNKIVL
                     LALWVPPAVALTFILSDRWFHLYYSSVKIATEDGGPLLIERGPFYIYALVCTYTLYLA
                     GLAALVRRFLVSRDIYRSQTPYLIGAVIIPFSLNIVYQVNPGILPIFPAPVDLTALAF
                     TFTAAFLATSIFGLRLLDLVPIARDNVIESIPEMVFVLDDKERIIDANAVAQQWLEKP
                     FAEILGKEPIEVFQKWPQLLQRFFFMERTREEIEIPGDPPRTLETIITP"
BASE COUNT          587 a          534 c          519 g          639 t
ORIGIN      
        1 atattcccat aaattggcgg ttccatgctt attcactcag cttctcaggt attgaccctc
       61 tccggcgggc cccagcgcgg aaaaatgctt ggcacattgg gcattattcc aaatggtgca
      121 gtggtcgtgc gcgatgaaaa aatcattgcg gtaggaacaa cggatgaatt acgaaacgcc
      181 taccctgatg agcctactct tgatgcaggt cggtgtgtgt taatgcccgg ctttgtagac
      241 ccgcacaccc atcttgtttg ggcaggtgac cgagcagatg agtttgagaa aaaaatggca
      301 gggacaccgt accttgatat tctcgccgca ggtggtggca ttctctctac ggttcgagcc
      361 acacgcactg ccagccttga aacacttatg gcagaaaccc gtccgcgcat gttgaggatg
      421 ttcagaaatg gcaccacaac tgcagaagca aagaccgggt atgggcttca aacgtctaca
      481 gaattacgtc tgcttaaggc tttgttagca ttgaatgatg aaagccccat ggatcttgtt
      541 tttactttct tgggagctca tgctattgca ccagaattta aggataatcc gcaagggtat
      601 acagacgaag taatcaacac catgctgccc atgctcaaaa gctggtggga gactcatgct
      661 ccgcggcaca ccctgccctt tgtagatgtt ttctgtgaga cgaaagcttt cacccttgaa
      721 caagcgcgtc agattctgac gcaagcaatg gcattgggtt ttccgctaaa gattcatgca
      781 gatgaattcg ataatcttgg tggagcagcg cttgcagcgg agttaaaggc tgcttccgct
      841 gaccatctcg tggtcacgtc tgatgcggat attgaagcgc taggaaagtc agacacagtc
      901 gcagtcgcgc ttccatgtac tccattcggg ttggcagagg cgcactatac tcctgcaaaa
      961 aaacttatcg aagccgatgc gcttttagct gtggctacgg actgtaaccc cggcacctca
     1021 tggaacgagt caatgcaatt cgcaattgcg ctggcatgcc gctacatgaa attaacgcct
     1081 gcccaagcca ttgcagccgc aacgatcaat gccgcacatg ctattcgaaa ggcagatatg
     1141 attgggtcca ttgaagtagg caaacaagcg gatatgttga ttttatcggt caatgactat
     1201 cgacaggtag gctatcgcta tgggacaaat ctcgtcaaac agatcatcaa acgtggacaa
     1261 gtctactccg tggatgttgg ctactatagg accgcgtaat tattatgaat aactcttgga
     1321 gtattttcgc gggcattttg atcttcaatg caattacggc gttagctatt gcttttattc
     1381 tcacacgtag acctcaaaaa aatggcatta aacccttgac cttgatgttc atttcgctgg
     1441 cattttggtc ttttgggtat gccatgatca ctttatcgcc acaactgaaa gacaagatct
     1501 tttggctcaa atttgaaaat tttggcattc agaccactgc actgttttgg ttcctgtttg
     1561 caatgatgta caccaaaatg gatagttgga tggataacaa gattgtactc ttggcattgt
     1621 gggtgccccc agctgtagca ttaactttta tcctgtctga ccgatggttc cacttgtatt
     1681 acagcagcgt gaaaatcgct actgaagatg gtggacctct tcttatagag cgtggtcctt
     1741 tctacattta cgccttggta tgtacataca cgctttatct tgcagggttg gcagcactgg
     1801 tgcgacgttt tttagtctcc cgagatatat accgaagcca aacaccttat ttaattggcg
     1861 ccgttattat tccattttct cttaatattg tttatcaagt aaatcctggt attttgccaa
     1921 tctttcctgc tcctgtggac ttaaccgctt tagctttcac attcacagcg gcatttcttg
     1981 ctaccagtat ttttggtcta cgtctgctag accttgtgcc aattgcacgc gataacgtga
     2041 ttgaatctat acctgaaatg gtattcgttt tagatgacaa agaaagaatc attgacgcaa
     2101 atgccgtggc tcaacaatgg ttggaaaaac cttttgcaga gattctagga aaagaaccaa
     2161 ttgaagtctt tcagaaatgg ccccaattac ttcagcggtt tttctttatg gaacgtacac
     2221 gtgaagagat cgaaattccc ggcgacccgc cccgcacact tgaaaccatc atcaccccc
//
LOCUS       DAOQJE010000648         2276 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_36054_length_2276_cov_4.410176, whole genome shotgun sequence.
ACCESSION   DAOQJE010000648 DAOQJE010000000
VERSION     DAOQJE010000648.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2276)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2276)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2276
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36054_length_2276_cov_4.410176"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..754)
                     /locus_tag="PLT08_14690"
     CDS             complement(<1..754)
                     /locus_tag="PLT08_14690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE05771.1"
                     /translation="MNNSPVIKFDLIEWREQFTRIVLQIASVLGIFLIVVSFPTASFT
                     DKILFISVYIVLLIVTLLPTPYLLRVYLLLIAIMAIGVNAILAWGPWADGTIFLLAAV
                     VLASLLLDNRTDSLLLTAISIFLISSAFLTQLGIFQLSAPDVPKTNVESWVVFITDYI
                     AIGIVIVVAANMLKRAFSRVANQMQKTFETLEIERRNLENKVQERTVELETRMAQLRA
                     STLTARAIAETQDIAELLKNAANLIAERFEYYH"
     gene            complement(747..>2276)
                     /locus_tag="PLT08_14695"
     CDS             complement(747..>2276)
                     /locus_tag="PLT08_14695"
                     /inference="COORDINATES: protein motif:HMM:NF024583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HNE05772.1"
                     /translation="VYRTWREGFIISLLVGVLIFGAVALLPALNASESIVIDGIFIST
                     YIIVALATIFKFPYNIRVTAFLLSVYVLGIGELISLGILGDGLFYFIALVVFSTMLVS
                     PRAGSVSIVISIITFIVVGWLMISGRFDPLFPVVAPAKLADWFSASAALIMFGAVIII
                     GFGQLEKEFAGAQAQITSTLDELREERNNLDNRVQERTNQLRRINEVGRAVAAILDIN
                     EILPLAAKYIQNEFDFYYTAVYLLDVTGQWADLKEAIGETGKVMKERKHRVNTNGKSL
                     VAQVIRSKTGQIMQDTNQIRQENPMFPYTRSLIVVPLVVGDTILGALEMHSSKDNEFL
                     PQDLDAYQNMANGISIAIENARLFQEAQQSIMEMRATQRQYLETAWSSLASEKSLDYA
                     LGDADLADTNSLEVPLALRNQTIGQIFTAGSSEWTNEQKSLIEAIAAQATLALENARL
                     VENSQFTAAQERLTNEIIAKIWAASNLDGILQTTVRELGRNLEASEVEIEVSMEDLPD
                     E"
BASE COUNT          694 a          486 c          466 g          630 t
ORIGIN      
        1 cgtggtaata ctcgaaacgt tcagcgatta aatttgcagc attttttaac aattcggcaa
       61 tatcttgtgt ttcagcaatt gcacgagcag ttagggtaga ggcgcgtagt tgcgccatgc
      121 gagtctctaa ctcaaccgta cgttcctgca ctttgttttc aaggtttcgg cgttctattt
      181 ccagtgtttc gaaagtcttt tgcatttgat ttgcaactct cgaaaaggca cgctttaaca
      241 tgtttgcggc aacaacaata acaataccaa ttgctatgta atcagtaata aagacaaccc
      301 aactttcaac gttggttttt ggaacatcag gtgctgataa ttgaaaaatc cccagttgtg
      361 tcaaaaacgc acttgagatt aaaaatattg atattgcggt taagagcagg gaatcagtcc
      421 tgttgtctaa caacaacgaa gcaagaacta ctgctgctaa taaaaagatg gtcccatctg
      481 cccatggtcc ccaagccaga atagcattga cgccaatagc cataattgca attaacaaca
      541 aataaacacg cagtaaataa ggtgttggaa gaagagtaac aatgagcaag acaatgtata
      601 ccgaaatgaa cagtatcttg tctgtaaatg aagcggttgg aaaagatacc actatcaaaa
      661 aaatgcctaa aacagaggca atctgcagaa caatacgagt gaattgttca cgccattcta
      721 ttaagtcaaa cttgataaca ggactattat tcatcaggca agtcctccat ggaaacttcg
      781 atttccactt cagaagcttc gagatttctt ccaagttcac ggacggttgt ctgtaaaata
      841 ccgtccagat ttgaagcagc ccaaatcttg gcaataattt cgtttgttaa tctttcttga
      901 gccgcagtaa attgactgtt ttctacaaga cgggcgtttt ccaatgcgag agtggcttgt
      961 gctgcgatgg cttcaatcaa acttttttgc tcattggtcc attcgctaga tccagcagta
     1021 aaaatctgac caatggtctg gtttctaagt gcaaggggca cttcaagcga atttgtatct
     1081 gctaaatcag catcacccaa ggcatagtcc agagattttt cagaggcgag tgagctccag
     1141 gcagtttcaa gatactgtct ttgagttgct ctcatttcca taatactttg ttgagcttct
     1201 tggaaaaggc gggcattttc aatcgcgatt gaaattccat ttgccatatt ttgataagca
     1261 tcaagatctt gtggcaaaaa ttcattgtct tttgatgaat gcatctcaag agcgcctagt
     1321 atcgtatcgc caactactaa aggcactacg atcagagatc gtgtatatgg gaacatcggg
     1381 ttttcttggc gaatttgatt ggtgtcttgc atgatctgtc ccgttttcga acggatcact
     1441 tgggcaacca aacttttccc gttggtattg acgcgatgct tgcgttcctt catcaccttg
     1501 cctgtttcgc caatagcttc ttttagatca gcccattgtc cggttacatc caaaagataa
     1561 acagcagtgt aatagaaatc aaattcgttt tgaatatatt ttgcggcgag tggcaatatc
     1621 tcattaatat caagaatggc agccactgca cgccctactt cattgatgcg tcggagttgg
     1681 ttggtgcgtt cctgtactct attatcgaga ttatttcttt cttcacgtaa ttcatcgagc
     1741 gtagatgtga tttgtgcttg tgcgccggca aactcttttt ccagttgtcc aaagccaatg
     1801 ataatgactg ctccaaacat aatgagtgca gcgctggcac tgaaccagtc tgcaagcttg
     1861 gcaggagcaa caacagggaa gagtgggtca aaacgtccac taatcatgag ccaaccgact
     1921 acgataaaag taataatact aattacgatt gagacactac cagctctagg cgacaccagc
     1981 attgtagaaa aaacaactag cgcaatgaaa tagaaaagac catctcctaa aattccaaga
     2041 ctgatgagtt ccccaatgcc taatacatac acactcaaca agaacgcggt cacgcgaatg
     2101 ttgtaaggga acttgaaaat cgtagcaaga gccacgatga tataggtcga tataaagatg
     2161 ccgtcgatga caatgctttc agaagcattg agcgcaggca aaagtgccac agcaccaaat
     2221 atcagcacac caaccaaaag ggaaataata aaaccttctc gccaggttct ataaac
//
LOCUS       DAOQJE010000649         2271 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_36212_length_2271_cov_9.348827, whole genome shotgun sequence.
ACCESSION   DAOQJE010000649 DAOQJE010000000
VERSION     DAOQJE010000649.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2271)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2271)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2271
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36212_length_2271_cov_9.348827"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..559
                     /locus_tag="PLT08_14700"
     CDS             <1..559
                     /locus_tag="PLT08_14700"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Maf family protein"
                     /protein_id="HNE05773.1"
                     /translation="TPFEHESPRDYVIRLAQAKASAVQPKAGLDAVILGSDTTVVDGN
                     EILGKPVDESDAWRMLKQLRGRTHQVYTAIAILQHGKMYTDLSIIDVPMREYSDQEID
                     TYIKTGDPMDKAGAYAIQHPEFKPVSSLEGCRAGVMGLPMCHVVRTLKHFNISSFADV
                     PSACQSLLQYDCKVSPLILSGEIVN"
     gene            578..>2271
                     /locus_tag="PLT08_14705"
     CDS             578..>2271
                     /locus_tag="PLT08_14705"
                     /inference="COORDINATES: protein motif:HMM:NF013100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HNE05774.1"
                     /translation="MKSLRWKQLALVALIIVGCTGPGSGSGVGSIFATDTPLPTPSVG
                     ITPAPNVDAAVTAFFDALKGDNYEEMYAMLSQSSRDALTLEDFSKRWTNALNEMSASS
                     FDVTVLSKSITPFNAEVKYSITYKTALVGDIQREMLMRLTNEDSNWKVQWDDALILPE
                     LAGGNLLKMEYSIPARGDIYDSKGLPIATQSDAFAFGIQTDQIDYDMIGALTTELGRL
                     CGLDPLDIQAQIDAAGPGWYLPMCEGTREEAQRLLSINPGGLVIQEYNSRYYFRGGLA
                     PQAVGYTLAISPEQLNQYKRLGYSGSERIGYTGVEQWAEDYLAGKHGGILRVVSPTGQ
                     VIATLGQSQPAPADSVYLTLDSNMQYYAQAAVEYFRGAIVVMEVDTGRIIAMASGPDF
                     DPNVFDSNNRNNQAVGNIFNDTRNPLLNRAAQGQLPLGSVFKIITFSAALESGLFLKD
                     TTYNCQYDFTELQDQVRHDWTWQHCQDRLAAGLFCNTSDSTPSGLLTLQEGLMRSCNP
                     YFWHIGNDLFTNWDRGNDIANMARAFGLGSPTGIQQIPEEAGQIDDPATQVDAVNQA"
BASE COUNT          575 a          521 c          572 g          603 t
ORIGIN      
        1 gactccgttt gagcatgagt caccacggga ttatgtgatc agactggcac aagccaaagc
       61 ttcagcggtt caacccaagg cggggctgga tgcggttatt ctggggtcag atactacggt
      121 ggtagatggg aacgagatcc tcgggaaacc tgtcgatgaa tctgatgcgt ggcgcatgtt
      181 aaagcaattg cgcgggcgga cgcatcaggt ctacaccgcg attgccatcc ttcaacatgg
      241 aaagatgtat acagacttgt ccattattga tgtgcccatg cgcgaatact cagaccaaga
      301 aattgacacc tatatcaaaa ccggcgaccc catggataaa gctggtgcat atgccattca
      361 acacccggag tttaagccgg tctcttcatt ggaaggttgc cgtgccgggg tgatgggttt
      421 gcctatgtgt catgtggtgc gcactttgaa acactttaat atttcatctt ttgcagatgt
      481 tccatctgca tgccaatctc ttttacagta tgattgtaag gtctcgccgt tgatcttgag
      541 cggtgagatc gtcaattaga aattgaaagg aaacgtaatg aagtcattac gctggaaaca
      601 attagctctc gttgctttaa tcattgtcgg ctgcaccgga cctggctcag ggagtggagt
      661 cggttcgatt tttgcaaccg atacaccctt gccaaccccg tcggttggaa ttacccccgc
      721 tcctaacgtg gatgcagcag tcactgcgtt ttttgatgcg ctaaaaggtg acaattatga
      781 agagatgtat gccatgttgt cacagtccag ccgcgatgcg ttgaccttgg aagatttctc
      841 aaagcgttgg acgaatgctc taaatgaaat gagcgcatcc agttttgatg tgactgttct
      901 ctcaaaatcc attacgcctt ttaatgcaga agttaaatac agtattacgt ataagactgc
      961 ccttgttggc gacattcaac gtgaaatgtt gatgcgcctt acgaatgaag atagcaattg
     1021 gaaagtgcaa tgggacgacg ccctgatcct tcctgaattg gctggcggca acttactaaa
     1081 gatggaatat agcatccctg cccgtgggga tatctacgat agcaaaggct tgccaattgc
     1141 cactcaatct gatgcgtttg cttttgggat tcaaaccgat caaattgact atgacatgat
     1201 cggcgcattg acaaccgaac ttgggcgttt atgtggcttg gatccgcttg atatacaggc
     1261 gcagatcgat gctgccggac ccggttggta tctgccgatg tgtgaaggaa cgcgtgaaga
     1321 agcccagcgt ttactctcta ttaaccctgg tggtttggtc attcaagaat ataactcacg
     1381 ctattacttc cgcggtgggt tggctccgca agccgtggga tacaccctgg cgatttctcc
     1441 agaacaactt aatcaataca agcgtttagg ctatagtggt agtgagcgaa ttggttatac
     1501 cggcgttgag caatgggcag aagattattt agccggcaaa catggcggca ttttacgtgt
     1561 tgttagccca acggggcagg tgatagccac cttggggcaa agtcaacctg cgcccgctga
     1621 ttcggtatat ctcactctcg atagcaatat gcaatactac gctcaggcgg cagtggaata
     1681 tttccgtggt gccattgtag tgatggaagt cgataccggg cgtatcatcg ctatggcatc
     1741 gggacctgat tttgacccga atgtttttga tagcaataac cgcaataacc aagctgtagg
     1801 gaatattttt aatgacacta gaaacccgct tctgaaccgt gctgcccaag ggcaactccc
     1861 attaggatct gttttcaaga tcattacctt ttcggcagcc ctcgaaagcg gattgttctt
     1921 aaaagataca acatacaatt gtcagtatga ttttacagag ctacaagacc aagtgcggca
     1981 tgactggacg tggcaacact gtcaggaccg tcttgctgcc gggttattct gtaacacgtc
     2041 tgatagtaca ccttcgggtt tgcttacttt acaagaaggc ttgatgcgtt cttgtaaccc
     2101 gtacttctgg cacattggca acgatctctt cacaaactgg gatcgtggta acgacatcgc
     2161 gaacatggca cgtgcgtttg gtttgggttc tcccaccggc attcagcaga ttcctgaaga
     2221 agcaggtcag atcgatgacc cagccactca ggtagatgca gtcaaccaag c
//
LOCUS       DAOQJE010000650         2271 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_36215_length_2271_cov_4.441787, whole genome shotgun sequence.
ACCESSION   DAOQJE010000650 DAOQJE010000000
VERSION     DAOQJE010000650.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2271)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2271)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2271
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36215_length_2271_cov_4.441787"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..243)
                     /locus_tag="PLT08_14710"
     CDS             complement(<1..243)
                     /locus_tag="PLT08_14710"
                     /inference="COORDINATES: protein motif:HMM:NF020123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HNE05775.1"
                     /translation="MNRTQRIILYILLLIAGASWIVISGQPTGTTTVAAAAPQAGFTA
                     PDFTLKTAEGETYTLSELKGQAVLINVWATWCPPCKA"
     gene            311..1069
                     /locus_tag="PLT08_14715"
     CDS             311..1069
                     /locus_tag="PLT08_14715"
                     /inference="COORDINATES: protein motif:HMM:NF016163.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF429 domain-containing protein"
                     /protein_id="HNE05776.1"
                     /translation="MLFTDMVFVGIDAASSHKSYTYAALDKGMNLVALADGELDDVTT
                     FLGGQSSAIVAINAPSGVNRGLVREKKKDMFKSVKPRAAGFRVAELELRDRGITVSGT
                     PSTISACSAWVQSGFALYRKLEKMGFKKYPTENTPYQILESQPHAAFCVMAEAIPQPK
                     PSLEGKIQRQLLLYEAGVRIKDPMDFFEEITRHKMLKGNWPLDMLYQPEQLDALVAAY
                     TAWLSIHKPEKVSLIGDSTEGQIALPEKNLKEKY"
     gene            1091..>2271
                     /locus_tag="PLT08_14720"
     CDS             1091..>2271
                     /locus_tag="PLT08_14720"
                     /EC_number="2.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016744 - transketolase or transaldolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HNE05777.1"
                     /translation="MTNLEFQNKAINTLRFLSADGVQKANSGHPGLPMGAAAMAFTIW
                     TRHLRHSPRNPKWMGRDRFILSGGHGSMLLYSLLHLTGYDLSLEDLQNFRQWGSKTPG
                     HPEYGWTPGVEVTTGPLGAGLSTGVGMAIAGAHLAATFNKPGHELLDSYIYAIVTDGD
                     LMEGVASEAASLAGHLSLGKLIYLYDDNKISIDGSTDLAFTEDRAKRFEAYGWHIQRV
                     EDGNDVEAIDAAIKVAKADPRPSLILCRTIIGFGAPNRQGTQKAHGEPLGDEELNAAK
                     DNLGWPKEPRFLIPDDVLEFYRKAVDRGRELEADWKMRREAYARLFPDLGVEFNRRIL
                     MNKLPDGWESALPTFPADPKGMATRAASGKVINALAAKLPELIGGSADLAPSNNTKID
                     GS"
BASE COUNT          555 a          535 c          587 g          594 t
ORIGIN      
        1 ggctttgcat ggtgggcacc aagttgccca tacattaatg agaaccgcct gcccttttag
       61 ttcggagagg gtgtatgttt caccttctgc ggtttttagg gtaaagtctg gcgcggtaaa
      121 ccccgcttga ggggcggctg cggcgactgt ggttgttccg gttggctgac cggagattac
      181 gatccacgaa gccccggcaa taagcaaaag aatgtagaga atgattcgtt gggttcggtt
      241 catgagattt tagaagtaaa ataggactgt ttgttttgta ttcggtgtgt tcttaagaga
      301 taatcttact atgcttttta cagacatggt ttttgttggt attgacgcgg cttcttccca
      361 taagtcctat acgtatgctg cgctggataa ggggatgaat cttgtcgctc tggctgatgg
      421 cgagctggac gatgtgacga ccttcctggg tgggcaatct tcagcgattg ttgcgatcaa
      481 tgcgccttct ggcgtaaatc gtgggttggt gcgcgagaaa aagaaagaca tgtttaagtc
      541 ggtgaagccg cgtgcggctg gttttcgcgt ggcagaattg gaattgcgag accgcggcat
      601 tacggtttct ggtactcctt ctacaattag cgcttgctct gcatgggtac aatctggttt
      661 tgcactttat cgtaaactgg aaaagatggg cttcaagaaa tatccaactg aaaatacgcc
      721 ctatcaaatt ttagagtctc agccgcatgc tgctttttgt gtcatggctg aggcgatacc
      781 gcaaccaaag ccttcattgg aagggaaaat tcaacgtcaa ttgcttctgt atgaagccgg
      841 cgttcgtatc aaagacccga tggatttttt tgaagagatc acacgccata aaatgctaaa
      901 aggcaattgg ccgttagata tgctttatca gcctgagcaa ttggatgcat tggtggcagc
      961 ctatacggct tggctgtcaa ttcataaacc tgaaaaggtt tcccttattg gagattctac
     1021 agaagggcag atcgccctac ctgagaaaaa tttgaaagaa aaatactaac cgaatggtaa
     1081 aataccgccc atgacaaacc tcgaatttca aaacaaagca atcaatacac ttcgatttct
     1141 ttccgctgac ggcgtgcaaa aagcgaattc tggtcatccc ggtctcccga tgggagccgc
     1201 tgcgatggct ttcaccatct ggactcgcca tctgagacat agcccgcgca acccaaaatg
     1261 gatgggacgt gaccgcttca tcctttcggg tgggcatggt tccatgcttc tgtactctct
     1321 tctccatttg accgggtacg acctatcttt ggaagacttg cagaacttcc ggcagtgggg
     1381 cagcaaaacc cccgggcacc ccgaatacgg ttggactccc ggtgtggaag tgaccacagg
     1441 accgcttggc gcggggcttt caaccggcgt gggtatggcg attgccggag cacacttagc
     1501 tgccacgttc aacaaaccgg gtcatgaact acttgactcg tatatctatg ccattgtcac
     1561 cgatggcgat ttgatggaag gtgtcgcttc tgaagccgct tcgttggcgg ggcatctctc
     1621 acttggcaaa ctcatctatt tgtatgatga caacaaaatt tccattgacg gctcaaccga
     1681 ccttgccttt actgaagatc gtgccaaacg ctttgaagcc tatggctggc atattcagcg
     1741 tgtagaagat ggcaacgatg tagaagccat tgatgctgct atcaaagttg ccaaagcaga
     1801 cccacgcccg tcgttgattt tgtgtcgcac catcatcggc tttggtgccc cgaatcgaca
     1861 aggaacgcag aaagctcatg gcgaaccact gggcgatgaa gaacttaatg ctgcgaaaga
     1921 caacctcggc tggcccaaag aaccgcgttt cttgattcct gatgatgtgc ttgagttcta
     1981 tcgcaaagcc gtggatcgcg gacgtgaact cgaagccgat tggaagatgc gccgcgaagc
     2041 ttatgctcgc cttttcccag accttggtgt ggaattcaat cgacgcatat tgatgaataa
     2101 attacccgat gggtgggaat ctgcattacc gacattccct gctgacccga agggcatggc
     2161 aacccgtgcg gcttctggta aggtcattaa tgcattagcc gcaaaacttc ctgaactcat
     2221 tggtggctca gcagaccttg ctccttcgaa caataccaaa attgacggtt c
//
LOCUS       DAOQJE010000651         2268 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_36293_length_2268_cov_6.293719, whole genome shotgun sequence.
ACCESSION   DAOQJE010000651 DAOQJE010000000
VERSION     DAOQJE010000651.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2268)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2268)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2268
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36293_length_2268_cov_6.293719"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(83..544)
                     /locus_tag="PLT08_14725"
     CDS             complement(83..544)
                     /locus_tag="PLT08_14725"
                     /inference="COORDINATES: protein motif:HMM:NF025054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNE05778.1"
                     /translation="MLILTKTNSENKDFIHLIRFLDADLAQNNGDQNDFYAQFNKVNL
                     IKHVIVAYEDDKAVACGAIKEYEPGVMEVKRMYTSPESRGKGIAGKILAELEAWAVEL
                     NYHKCILETGKTQVSAIHLYKKSGYASIDNYGQYAGVENSVCFEKILAHKA"
     gene            complement(705..935)
                     /locus_tag="PLT08_14730"
     CDS             complement(705..935)
                     /locus_tag="PLT08_14730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE05779.1"
                     /translation="MPRIKCHYVDCVFLDEGLCGAAAVEFDPDTGCVTYSPADGAAAE
                     DEWEDEEELDEWEDEDADGEEDDEWLEEEDEF"
     gene            complement(947..1372)
                     /locus_tag="PLT08_14735"
     CDS             complement(947..1372)
                     /locus_tag="PLT08_14735"
                     /inference="COORDINATES: protein motif:HMM:NF019499.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin K epoxide reductase family protein"
                     /protein_id="HNE05780.1"
                     /translation="MDKWLYRVMIALVIIGLAVSVYMTIYKISGNDGMCLGSGDCSTV
                     NASRFSEVNGIPVAVFGIVGYAAILIVLFYENKNEFFKRNGTLLAFGMSLTGFLFTLW
                     LVYVELVLLKAICPFCVTSQVSMTLIFIIAVVRLFRNEN"
     gene            complement(1379..>2268)
                     /locus_tag="PLT08_14740"
     CDS             complement(1379..>2268)
                     /locus_tag="PLT08_14740"
                     /inference="COORDINATES: protein motif:HMM:TIGR00177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="molybdopterin molybdotransferase MoeA"
                     /protein_id="HNE05781.1"
                     /translation="DTDFNAKSAGTTAPKTVTFSKQMKSGENLRLRGADIRTGDAVLK
                     RGRTLKPQDLGLLATLGHAHVTVHKKARVALLSSGDELLDVSAPLEPGKIRDSNSYAL
                     AAAVSNLGVDVIRLGVARDTRESITQLLDQAASENADLILSSAGVSVGAFDYIKEVID
                     ANGKMDFWRVNMRPGKPLAFGEYRGIPFIGLPGNPVSAFVGFEVFVRPVLERLNGKMD
                     GGRQTVRVRCEEEIESDGRESYLRAKIQVKNNIRIAILTGHQGSGNLLSLVQADALLI
                     IPAGVKCVPAGSEVDAILL"
BASE COUNT          586 a          552 c          483 g          647 t
ORIGIN      
        1 attttagtta ttaaaaaaca ttatatataa attttgtgca atatcgcgca atcgcttttt
       61 tattaagatg ctttttatct tattacgctt tgtgagccag aattttttcg aaacagacac
      121 tgttttctac gcctgcatac tgtccgtaat tatcaataga tgcataaccg ctttttttat
      181 aaagatgaat ggcagatacc tgagttttac cagtttccaa aatgcacttg tgataattca
      241 actcaactgc ccacgcttct aactctgcaa ggatttttcc tgcaattcct ttgcctctgc
      301 tttcgggaga ggtgtacatg cgcttgactt ccatcacgcc gggttcgtat tccttgatcg
      361 cgccacaggc gacggctttg tcatcttcat atgcaacgat cacgtgtttg atcaggttga
      421 ctttattgaa ctgggcgtag aaatcatttt ggtcaccgtt attttgggca aggtctgcat
      481 ctaaaaagcg gatgaggtga ataaaatctt tattttctga gttggttttt gtaagtatta
      541 acataagatg ccctttttat gattttccct attttatact cttgataaaa tttcagggat
      601 aacaattgga gcattataca tacttgcgcc tggttaagaa aaagccctct cacatcgaga
      661 aggctttttt tgtagaatca gttttgagtt aagaatgaag cgggctagaa ttcatcttct
      721 tcttcgagcc actcgtcgtc ttcttcgcca tcggcgtctt catcctccca ctcgtcgagc
      781 tcttcttcat cttcccattc atcttccgct gcggcgccat cggcagggga ataggtcaca
      841 caacctgtgt ctgggtcgaa ttctacggct gctgcgccgc acaatccttc atccaaaaag
      901 acacaatcaa catagtgaca tttaatacgg ggcatttcga atcctcctaa ttctcgtttc
      961 ggaatagtcg tacaaccgca ataataaaga tcaacgtcat agagacttgc gaggtgacgc
     1021 agaacgggca gatcgctttg agcaatacaa gttcaacata taccaaccaa agcgtgaaca
     1081 agaaaccggt aagtgacatg ccaaaggcaa gcaaggttcc gttccttttg aaaaattcat
     1141 ttttgttctc gtagaacaaa acgatcaaga tggcagcgta gccgacaata ccaaatactg
     1201 ctacaggaat tccgttcact tctgaaaagc gactggcatt cacagtggag caatcaccag
     1261 agccgaggca catgccatcg tttccactga tcttataaat tgtcatataa actgacactg
     1321 ccaaaccgat gattacaagc gcgatcatca cgcgataaag ccatttatcc atggttcctc
     1381 acaatagaat agcgtctact tccgaaccag caggcacgca ttttacacca gcggggatga
     1441 ttagtaaagc atccgcctgt actagtgaca aaaggttacc cgagccttgg tgtccggtca
     1501 aaattgcaat acgaatgtta tttttgactt ggatttttgc ccgcagataa ctctcgcgac
     1561 catctgattc gatttcttct tcgcatcgta cgcggaccgt ttgcctgcct ccgtccattt
     1621 tgccgttcag ccgttcaagc acggggcgca caaaaacctc gaagccaaca aaggctgaaa
     1681 cggggttgcc cggcagccca atgaacggaa ttccccgata ttcgccaaat gccaatggtt
     1741 tgcctgggcg catgttaacc cgccaaaaat ccattttgcc gttggcatcg atcacttctt
     1801 tgatgtagtc aaaagcgcct acactcaccc cggcagagga aaggataagg tctgcgtttt
     1861 cacttgcggc ttgatccaaa agttgggtga tagattcgcg agtatctctt gcaacaccca
     1921 gccgaatgac atctacgcct aggttgctga ctgcggctgc caaggcatag gaattagagt
     1981 cacggatctt gccgggttcc aacggagcgc ttacatccaa taactcatct ccagaggata
     2041 acaatgccac tcgcgctttc ttgtggaccg ttacatgtgc atgtccaagt gtggcgagca
     2101 gacccaagtc ctgcggtttg agagtgcgac cccttttcag cacagcatct ccagtgcgga
     2161 tatctgcgcc tcgcaggcga aggttctctc ctgatttcat ctgtttggaa aaggtgactg
     2221 tcttgggggc ggtggttcct gcgcttttcg cgttgaaatc tgtgtctt
//
LOCUS       DAOQJE010000652         2267 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_36320_length_2267_cov_4.775769, whole genome shotgun sequence.
ACCESSION   DAOQJE010000652 DAOQJE010000000
VERSION     DAOQJE010000652.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2267)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2267)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2267
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36320_length_2267_cov_4.775769"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            372..1232
                     /locus_tag="PLT08_14745"
     CDS             372..1232
                     /locus_tag="PLT08_14745"
                     /inference="COORDINATES: protein motif:HMM:NF015686.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HNE05782.1"
                     /translation="MHFEFDWVSDPASWIALITLIALELVLGVDNIIFISILSGKLPK
                     EDQPRARTTGIMLAVITRIALLLSLSWIISLEEPFFYIPWFGEHGNIGISGRDLILIV
                     GGLFLLWKSTHEIHEKLEGKEGVASAKVAANFVSVIIQIMLLDIVFSLDSVITAVGMV
                     DHITIMIIAVIIAAIIMIFVAAPIGDFVEHHPTIKILALSFLLLIGFTLVVEGLHQHI
                     PKGYVYFAMGFSVMVEMINLRVRGKSEPVALHTPYVAEPVAVSASAAVSSPKKPAAKK
                     AKPASKKSKK"
     gene            complement(1264..2157)
                     /gene="thrB"
                     /locus_tag="PLT08_14750"
     CDS             complement(1264..2157)
                     /gene="thrB"
                     /locus_tag="PLT08_14750"
                     /EC_number="2.7.1.39"
                     /inference="COORDINATES: protein motif:HMM:NF002288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004413 - homoserine kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine kinase"
                     /protein_id="HNE05783.1"
                     /translation="MSRMIKIRVPATSANLGPGFDCLGLALDVWNEIAFEPAEQLKYR
                     ASGEGAEKLNKGNRNLLTKAYARLHEFCGKKASGVRIAARNDIPMSSGLGSSAAAIVA
                     GLFGANEMLGKPLHKDELVKLATEMEGHPDNVAPALLGGLVASVMKHEEIITRRYEVP
                     ALTVVIVKPDVEWLTKTARAVLPKSISRADAIFNIGRATLVVDALRTGDLDLLQKVMD
                     DRIHQPYRLRHISGGMAAYKCAKAFGAAALSGAGPSIILFTQPNQAERAKREIQAVFE
                     ERGIDTKGIITSPGKKGVHCI"
BASE COUNT          601 a          502 c          506 g          658 t
ORIGIN      
        1 atgatccaca aaaaaaggaa atttcagcat ggattcgaag tgccaattca tgaatctggc
       61 aatgaaactg acccagatgt tcaagatcaa gtaaatagag aaacagtatt gtaaaatacg
      121 ccctgcttga ttggcagggc gtatccagtt aaaagcgtta gctcagaaag aaataaatct
      181 ctttaaaaga actgacgctg aaaacctaaa ctcgctggtt ttcagcgtca gtggaacgtc
      241 atgagttaac cgacaaaatc aaagctaaca aggtttaata agattattta ggctcttgtt
      301 acagccaatc ttcccggctt ttttatcgaa tttggtaaaa ttgcctcaat cttttcgtag
      361 gagtagaagt aatgcatttt gaatttgact gggtgtccga tccagcatca tggattgcgc
      421 tgattacgct aatcgcgctt gaattagttt taggggtaga taatattatt tttatttcta
      481 ttctttctgg caaattgccg aaagaagatc aaccccgtgc ccgtaccacg ggtattatgc
      541 tggcggttat cacacgtatt gcattgttgc tctctctttc atggattatc agccttgaag
      601 aacctttctt ttatattcct tggtttggtg aacacggaaa cattggtatt tctggtcgtg
      661 atttgatcct tattgttggc ggacttttct tattatggaa gagtacccat gaaatccatg
      721 aaaaattgga aggcaaagaa ggggttgcat cagcgaaggt ggctgccaac tttgtgagtg
      781 ttatcatcca aattatgttg ttggatattg tcttctcact tgactccgtg atcaccgcag
      841 ttggaatggt tgaccacatt acgatcatga tcatcgctgt aatcattgcg gcaattatta
      901 tgatctttgt agcagcaccc attggcgatt ttgtggaaca tcatcctact attaagattt
      961 tagccttgag ttttctgttg ttgatcggtt ttacccttgt tgtagaaggg cttcatcagc
     1021 acataccaaa gggttatgta tatttcgcga tgggcttctc agtgatggtc gaaatgatca
     1081 acctccgtgt tcgtggcaag tctgagccag tggcgttgca tacaccctat gtagcagagc
     1141 ctgtcgcggt ttcagcctct gctgcggttt cttctccaaa gaaaccggct gccaagaagg
     1201 caaaacctgc tagcaagaag agtaaaaaat aaaacaaaaa gcggacttgg aaaagtccgc
     1261 tttttatatg cagtgaacac cttttttgcc agggctggta atgatccctt ttgtgtctat
     1321 tccacgttct tcgaatacag cttggatctc tctcttggcg cgttctgcct gattcggttg
     1381 cgtaaaaaga ataatagaag gacctgctcc agataaggca gccgcgccga atgccttggc
     1441 gcatttgtat gctgccattc cgccagaaat atgacgcaaa cgataaggct gatggatgcg
     1501 gtcatccatc actttttgga ggaggtcaag gtcaccagta cgaagcgcat ccacaaccag
     1561 tgttgctcga ccgatattaa aaatagcatc tgcgcgcgaa atagactttg gcaagacagc
     1621 ccgagctgtt ttggtcaacc attctacgtc tggtttgacg ataaccacgg ttaatgccgg
     1681 aacttcgtat ctgcgagtga tgatttcttc atgcttcatc accgaagcga ccaacccgcc
     1741 gagcaaagcg ggcgctacat tatctggatg tccctccatc tctgtcgcaa gctttaccaa
     1801 ttcatcctta tgaagcggtt tgccgagcat ttcatttgcg ccaaagagac ctgccacaat
     1861 cgcggcggcg cttgagccga gcccgctgct catcggaatg tcatttcgag cggcaatgcg
     1921 aactccgctt gcttttttgc cacaaaattc atgcaaacgt gcatacgcct ttgtgagcag
     1981 gttgcgattc cctttgttta acttttcagc cccttcaccg cttgcgcgat atttcaattg
     2041 ttcagcgggc tcaaatgcaa tttcattcca aacatccaat gccaaaccga ggcaatcaaa
     2101 accaggacca aggttcgcag atgtagctgg gacgcggatc tttatcatgc gggacattat
     2161 ataatacctg cgttgcaaat gtggaaaaat gtgcactttg taaccgggat gagtataatc
     2221 gcctcaatct ataactcgga gttgatatga cctaccaggg cttaatc
//
LOCUS       DAOQJE010000653         2261 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_36494_length_2261_cov_5.698096, whole genome shotgun sequence.
ACCESSION   DAOQJE010000653 DAOQJE010000000
VERSION     DAOQJE010000653.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2261)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2261)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2261
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36494_length_2261_cov_5.698096"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            72..386
                     /locus_tag="PLT08_14755"
                     /pseudo
     CDS             72..386
                     /locus_tag="PLT08_14755"
                     /inference="COORDINATES: protein motif:HMM:NF012447.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid lyase"
     gene            679..1641
                     /locus_tag="PLT08_14760"
     CDS             679..1641
                     /locus_tag="PLT08_14760"
                     /inference="COORDINATES: protein motif:HMM:NF016101.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HNE05784.1"
                     /translation="MSLFKKMIVIAVLLVLTGCDSFLGTSPTKIPPTSTSTPAPTALP
                     IPKPLVPNFDHIVVFMLENEDFEGVIGNVDMPVYNKLADENILLTQYYAVGHTSLVNY
                     IALISGETFGINTNCTDCYIDSPSLPDLIEASGRTWKTYQEDMPSPCFIGSDITYAQK
                     HNPFIYFDPIRLDTARCEKSVVPLSMLKNDIDAGKLPNFIFITPNICNDAHDCTLGVA
                     DQWLGKQLDVLIPALDAEGPNYMIISLFEEGYGNESCCGMPENNAGGHVPAVILSPMA
                     KSSFKDDMPYSHYSLLKTISNAWGLPYLGHAADEETSIMTSPWK"
     gene            1859..>2261
                     /locus_tag="PLT08_14765"
     CDS             1859..>2261
                     /locus_tag="PLT08_14765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE05785.1"
                     /translation="MTQDLQSQNMLMTGARQTMTAWSKVLESREEIPAIYQELFGKHF
                     AEGAPFPHVIWMPALNRTSGWTTEKLICDTPEVLHIFEKNGKQVSALSYAYEDVYSIE
                     DGNILLDAWLMVSGKTQGGEKRVTTLHFNMTS"
BASE COUNT          624 a          448 c          519 g          670 t
ORIGIN      
        1 atggcggtaa ccacagaccg cgagttgatc ttcattcagg agacggggaa cgataaggaa
       61 ctcaccccgc cagcgtggat acgattccaa cctccgccaa cgtggaggat catgtcagta
      121 tgggcgcgac agccgcggtc aagttgcgaa gcgtgctcga taacgtcgag cgaatccttg
      181 cgatcgagtt gatgtctgcc gcgcaaggtg tggattttcg caagcaagtg atcggcgaag
      241 aaaaacgttt gggcgaagga acaagaagcg tctacgccct gatccgcgaa cgcataccgt
      301 tcattgagtc agatacggtg atgtacgagt atatggagac ggttcgtcag cttgtggcgg
      361 atggtgaaat agttattaag agatgaattt gacaatgttc aattttatag attcgagtta
      421 cattatttga atgaataact ggtataagag aaatacctgc ctcattacat ctaacataat
      481 tgtatggtaa acgacaaaat ctttttaatt taatcctaat gtattgggat tattctattt
      541 gttgtattct tcccattatt cgtatcttca taaataattc ataatttaat cgcatcacat
      601 aaaccaattt tggtgcgcat gttgttgcct gtctatattg caggaatatg cttgccttct
      661 agtatatatg gagaaaaaat gtcccttttc aaaaagatga tagtcattgc agttttgtta
      721 gtattgactg gttgtgactc ctttttaggc acttcaccaa caaagattcc gcctacctca
      781 acttcaaccc ctgccccaac tgcccttccc attcctaaac ctttagtccc aaactttgat
      841 catattgtcg tttttatgct tgagaatgaa gattttgaag gtgtaattgg aaacgtagac
      901 atgcccgtct ataataaact tgccgatgaa aatattctac tcactcagta ttatgccgtc
      961 ggacatacaa gcttggtgaa ctatatcgct ttaatcagtg gtgagacttt cggtatcaat
     1021 accaattgta ctgattgtta tattgattct cccagtttgc ctgatctgat tgaagccagt
     1081 ggacgaactt ggaagacata tcaagaagat atgccttctc cttgttttat cgggagtgat
     1141 ataacatatg ctcagaagca taatcctttt atatactttg accctatccg gttggataca
     1201 gcccgttgtg agaaaagtgt tgtcccatta agtatgctaa agaatgacat tgatgctggt
     1261 aaactaccca attttatatt tatcacccct aacatctgca atgatgctca tgattgcact
     1321 ttaggtgtgg cagaccagtg gctcggcaag cagcttgatg ttctcatccc cgccttggac
     1381 gcagagggac ctaactatat gattattagc ttatttgagg aagggtacgg aaatgaatca
     1441 tgttgtggaa tgcctgaaaa caatgcgggt gggcatgttc cagccgtgat tctttctcca
     1501 atggctaaaa gtagtttcaa ggacgatatg ccttattcac attattccct tctgaaaacg
     1561 atatccaatg cttggggact accctatctt gggcatgctg cagatgaaga gacatctatc
     1621 atgacttcac cttggaaata aaaaaaattt agatgactta atgactgtca atagaaaatt
     1681 atctttgagc tacgtatgta gacggttcgt ccactggtag cagacggata agtcatcacg
     1741 aattgatggt tttcatggac gggaaatttt cccgtccatg tttattttga atatgcttgt
     1801 ctcttttgtc ttttttcttg ggggtacaat gtttgcatca acgatataga ggcattccat
     1861 gacccaagat ttgcaaagcc aaaatatgct tatgaccggc gcgcgtcaga cgatgacggc
     1921 gtggtcgaag gtgcttgaat cgcgagagga gattccggcg atctatcagg agctgtttgg
     1981 caaacacttt gcagagggag cgccgttccc gcatgtgatt tggatgcctg ctttgaatag
     2041 aacttctggg tggacgactg agaaacttat ttgcgatacg ccagaggtat tgcatatctt
     2101 tgaaaaaaat ggcaaacagg tcagtgcgct ttcgtatgcg tatgaagatg tgtatagcat
     2161 cgaagatgga aacatcctgt tggatgcgtg gctgatggtg agtggtaaga ctcagggcgg
     2221 tgagaagcgg gtgacgacgc ttcacttcaa catgaccagc a
//
LOCUS       DAOQJE010000654         2258 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_36575_length_2258_cov_5.266001, whole genome shotgun sequence.
ACCESSION   DAOQJE010000654 DAOQJE010000000
VERSION     DAOQJE010000654.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2258)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2258)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2258
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36575_length_2258_cov_5.266001"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            9..260
                     /gene="infA"
                     /locus_tag="PLT08_14770"
     CDS             9..260
                     /gene="infA"
                     /locus_tag="PLT08_14770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012039277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HNE05786.1"
                     /translation="MTKEEGKIRVDGVVIEALPGTQFRVKLDNGHEVLAYLSGKMRKH
                     YIRILLGDRVALEMSPYDMSRGRITFRQRKQAAAAPVEE"
     gene            complement(321..1322)
                     /locus_tag="PLT08_14775"
     CDS             complement(321..1322)
                     /locus_tag="PLT08_14775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase-like protein"
                     /protein_id="HNE05787.1"
                     /translation="MTELSTTYLGLKLKNPLVASASPLSKKTDKAKKLEEAGISAIVM
                     YSLFEEQIIHESLELDHFLSRGTDSFAEALTYLPDGGMYSVSPEKYLNQVTSLKKAVN
                     VPVIGSLNGVSKGGWTSYAKRIEEAGADALELNLYFLSTDPNLTAAALETAQAELVAE
                     VKSSIKIPLAVKLSPFVTSLPNFAKRIVDAGADGLVLFNRFYQPDFDLDNLDIIHSLD
                     LSTSADLRLPLRWISILHSNVHTDYALTSGIHTAHDVLKAMMAGAKVAMMASTLLHSG
                     ETVVSSMLNDLQTWMKEREYESIQQMQGSMSQKNVKEPAAFERANYMKVLGSWRDLP"
     gene            complement(1385..>2258)
                     /locus_tag="PLT08_14780"
     CDS             complement(1385..>2258)
                     /locus_tag="PLT08_14780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HNE05788.1"
                     /translation="QKEYAEELLKSFEQTVGTQLVTEILHANQADEAGIDAQRVRVED
                     LKSKLAKSSDSRAKDLISLAENFVKKSVWIIGGDGWAYDIGYGGLDHVLASGRNVNIL
                     VLDTEVYSNTGGQSSKSTPRAAVAKFSMGGKGMPKKDLGLIAMSYGYVYVAKVSMGAN
                     DQQTLKALLEAESYDGPSLVIAYSPCIAHGFDMARSLDQAKLAVQSGHWPIFRYDPRL
                     AIDGKNPLQIESKEPSIPFTQYAYNETRYKMLTQMNEERAEELMKEAQHDAKTRWTLY
                     QQMASMHYESDKKE"
BASE COUNT          509 a          522 c          613 g          614 t
ORIGIN      
        1 aggcatgtat gactaaagaa gaaggcaaaa tcagagtgga cggtgtggtg attgaagcgc
       61 tgcccggcac ccagttccgt gtgaagctcg acaatggtca cgaggttctg gcgtaccttt
      121 cgggtaagat gcgcaagcac tacattcgta ttttgttggg tgaccgtgtt gccctcgaaa
      181 tgtcccctta tgatatgtcg cgcggacgca tcacgttccg tcagcgcaag caagctgccg
      241 ccgctcctgt cgaagaatag tccttgcgat ttgaaataaa aaaggctctc catttggaga
      301 gcctttttcg ttattaagtg ctatggcaaa tcacgccagg agccgaggac cttcatgtaa
      361 tttgcacgtt caaatgccgc cggttctttg acgttcttct ggctcatgct gccttgcatt
      421 tgctgaatgg attcgtattc gcgttccttc atccaggttt gcaaatcgtt tagcatggaa
      481 gagacaactg tctcgccgct gtgcagtagg gtcgatgcca tcatggcgac ctttgcgcct
      541 gccatcatgg ctttgagtac gtcatgagcg gtgtggatgc cgctggtaag ggcgtaatct
      601 gtgtggacgt tgctgtgcaa aattgagatc cagcgcaggg ggaggcgtaa gtctgcagag
      661 gtgctcaaat ccaagctgtg gatgatatcc agattatcga ggtcgaaatc gggttggtag
      721 aagcggttga agagcacaag tccgtctgcg ccggcgtcca cgatgcgttt ggcgaaattg
      781 ggcagagatg tcacgaaggg actaagcttc accgccagcg gaatcttgat ggaggatttt
      841 acttccgcta ctaattccgc ttgggcggtt tcaagtgcgg cggcggttag gtttgggtct
      901 gtagagagaa agtataggtt aagttcgagc gcatcggctc cagcttcttc aatgcgtttg
      961 gcgtagctgg tccaaccgcc tttggagacg ccattcaagc tgccgattac ggggacattg
     1021 accgctttct tcaaactggt cacctgatta agatattttt cagggctcac gctatacata
     1081 ccgccatcag gtagataggt aagggcttca gcaaaagagt cggtgccgcg ggaaaggaag
     1141 tggtctaact caaggctttc gtgaatgatc tgctcttcaa agagagaata catcacgatc
     1201 gcggagatgc ccgcttcttc taacttttta gctttgtcgg tcttcttaga gaggggtgaa
     1261 gccgaagcca caagcggatt ttttagtttt aagccaaggt acgtagtgga aagttcagtc
     1321 atgggtaaag ttcctttagg gagttaagaa tgggcgagaa acgttctcgc ccataattta
     1381 aagtttattc ttttttgtcg ctttcgtaat gcatggatgc catttgttga taaagcgtcc
     1441 aacgggtttt ggcatcatgc tgggcttctt tcatgagttc ttcggctcgt tcctcattca
     1501 tttgagtgag catcttatac cgggtttcgt tataagcata ctgcgtgaat gggatgctgg
     1561 gttcctttga ctcgatctga agcggatttt taccgtctat tgccaagcgc ggatcgtagc
     1621 ggaagatggg ccagtgtcca gattgtacag cgagctttgc ttggtcgagg ctgcgagcca
     1681 tatcgaagcc gtgtgcaata cacgggctgt atgcaatgac gagggaaggt ccatcatacg
     1741 attcggcttc caacagagct ttcaaagtct gctggtcgtt ggcgcccatg gacaccttgg
     1801 caacatatac gtagccgtac gacatggcga tcagaccgag gtctttcttg ggcattccct
     1861 tgccgcccat ggagaacttg gcaactgctg cacgcggtgt ggacttggag gactgaccac
     1921 ctgtattcga atacacttcg gtatcgagca cgagaatgtt gacattgcgt ccactggcga
     1981 ggacatggtc caaaccgccg tagccaatgt cgtatgccca accgtcacca ccaattatcc
     2041 aaaccgattt cttgacgaaa ttctctgcca aactaatcaa gtctttggca cggctatcgc
     2101 ttgatttcgc aagtttcgat ttcagatctt ccacgcgaac gcgttgagca tcaataccgg
     2161 cttcatcggc ttggttggcg tgcaaaatct cggttaccaa ctgggtgcct accgtctgct
     2221 caaaggattt gagcaattct tcggcatatt ctttttgc
//
LOCUS       DAOQJE010000655         2256 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_36639_length_2256_cov_4.794184, whole genome shotgun sequence.
ACCESSION   DAOQJE010000655 DAOQJE010000000
VERSION     DAOQJE010000655.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2256)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2256)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2256
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36639_length_2256_cov_4.794184"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..504)
                     /locus_tag="PLT08_14785"
     CDS             complement(<1..504)
                     /locus_tag="PLT08_14785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE05789.1"
                     /translation="MSRQKAFLSLCLVLVLALACNLPSATPTETAAANPEDLELTITA
                     LASGQGNQPSDSPAQDTATPEFTPITGPTSTPTVPQVTVSQNTNCRVGPGVVYDLIDA
                     VLIGQTAEVVGKNSGVPNYWVVKRLNGSGTCWLWGQYATVSGNTANLPEYPVPPTPTP
                     SPTPTFTP"
     gene            complement(618..1406)
                     /locus_tag="PLT08_14790"
     CDS             complement(618..1406)
                     /locus_tag="PLT08_14790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE05790.1"
                     /translation="MFRRKITLFVCIFLVGALSACNLPSSAPTETETSELDLALTITA
                     LASSPNAPQETSTPEFTATAGPTSTPSVPQVSVSQNTNCRVGPGIVYDLIDSLLIGQT
                     AEIVGKNSGVPNYWVIKRINGSGTCWLWGEYATVTGNTANLPEYPVPPTPTPSPTPTF
                     TPTATPSAPAAVENVTMTMSTCGGVAPYQHGGTLAWEDKSNNEDGFNIYVNNVLIASI
                     PANSTSYNVAPAGSFAAGIASKFEVEAFNGVGKAAKKSVAKGCP"
     gene            complement(1523..>2256)
                     /locus_tag="PLT08_14795"
     CDS             complement(1523..>2256)
                     /locus_tag="PLT08_14795"
                     /inference="COORDINATES: protein motif:HMM:NF024258.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HNE05791.1"
                     /translation="IAVISDIHGNILALEKVVQDIKRRGVDQVVNLGDHVSGPLWAKE
                     TIQFLMEQSWVQIRGNHDRQLVSQAPETHGASDKFAFQQLNENELDWLRKLPPQAELS
                     GGILFVHGTPKSDNTYLLETIAHGSNRIATRNEIKERIGDSQYKLILCGHTHIPRVVA
                     ISEQTTIINPGSVGLQAYDDTSPEYHIMQTGSPHARYVILEKVDINWIVNLLHVEYDH
                     NSAAAHAHKNGRIEWEYALRTGLMP"
BASE COUNT          576 a          468 c          550 g          662 t
ORIGIN      
        1 aggtgtaaat gtcggagttg gagaaggtgt cggcgtgggc ggaaccggat attctggcaa
       61 attcgctgtg ttaccgctaa cggtggcata ttgcccccag agccagcagg ttccagaccc
      121 gttcaggcgt ttgactaccc aataattcgg gacgccggag tttttaccaa ctacttctgc
      181 tgtttgccca atgagtactg catcgattag gtcatataca acccccggac caacacgaca
      241 atttgtattt tggctgaccg tgacttgtgg cacagttggc gtagacgttg gacctgttat
      301 aggggtaaat tcgggtgtgg cggtatcttg agcaggggag tcgctgggtt gatttccctg
      361 tccgcttgct aaggctgtaa tggttaactc taagtcttca gggttggctg ccgcagtttc
      421 ggttggggta gcacttggca agttacatgc aagtgcaagg actaagacta aacacaaaga
      481 caaaaaagct ttttgacgtg acataaaacc tctctttcat atacctatta ttgcacacct
      541 aagaaaatgt attgtaaaac taacatgtta aagtataaat ctggcaggga tttccctgcc
      601 agatttatga gaaaacttta tgggcaaccc tttgcaacac tctttttagc tgcctttcca
      661 acaccattga aggcttccac ttcgaatttt gaagcaattc ctgcggcaaa cgagccggct
      721 ggcgcaacat tgtacgaagt gctattcgct gggatgctgg caattaagac attgtttaca
      781 taaatattga aaccgtcttc attgttggat ttatcttccc atgctagagt accaccatgt
      841 tgataggggg ctactccacc acaagttgac atggtcattg tcacgttttc aactgcggca
      901 ggcgctgaag gtgttgctgt aggtgtaaat gtcggagttg gagaaggtgt cggtgtggga
      961 ggaaccggat attctggcag attcgcagtg tttcccgtta cggtggcgta ctcgccccag
     1021 agccaacaag ttccagatcc attgatgcgt ttgatgaccc aatagttggg aacgccagaa
     1081 tttttaccaa cgatttctgc tgtttgacca atgagcaggc tatcaattaa atcatagaca
     1141 atgccaggac ctacacggca atttgtattt tgactaaccg atacctgtgg aacagagggt
     1201 gtagatgtgg gacccgctgt ggcagtaaac tctggcgtgg aggtttcctg cggtgcattc
     1261 ggactgctgg caagagccgt aatcgtgagt gcaagatcga gttctgaagt ttcagtctca
     1321 gtgggagcac tgcttggaag gttacaagca gaaagagcac caactaagaa tatacaaacg
     1381 aataaagtta tttttctgcg aaacataatt ctctccttta taagaaacta ttatggcaca
     1441 actttgatat atatcaataa aagggcttgt gagattagta ctagtgtgca aagggacgtt
     1501 ggtactagta ctagcttttg ttctagggca ttaaaccggt tcttaacgca tattcccatt
     1561 caatccgccc atttttgtga gcgtgtgctg cagcagagtt atggtcatac tcaacatgaa
     1621 gtagattcac gatccaatta atgtcaacct tttcaagaat tacatacctt gcatgtggcg
     1681 aacctgtttg catgatgtga tattctgggc tggtatcatc gtatgcttga agccccacac
     1741 taccggggtt tatgatcgta gtttgttctg agattgcaac tactctggga atatgagtgt
     1801 gtccgcagag aataagcttg tattgagaat cgccaattcg ttcttttatc tcattccgtg
     1861 tagcaattct attgctgcca tgagcgatgg tttcaagtag gtaggtatta tcactttttg
     1921 gtgttccatg gacgaataga attccgcctg acaattctgc ttggggaggt aatttgcgaa
     1981 gccaatctaa ttcattttca ttcaattgtt gaaaggcaaa cttatcagag gctccatgtg
     2041 tctctggggc ttgtgagacg agttgtctgt cgtggtttcc gcggatttgg acccagctct
     2101 gttccattaa gaattggatg gtttccttcg cccaaagcgg acctgataca tggtcgccca
     2161 agttgacgac ttgatctaca cctcgtcttt taatatcttg gacaactttt tcaagggcaa
     2221 ggatgtttcc atgaatatct gaaatgacag cgattc
//
LOCUS       DAOQJE010000656         2255 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_36669_length_2255_cov_8.210000, whole genome shotgun sequence.
ACCESSION   DAOQJE010000656 DAOQJE010000000
VERSION     DAOQJE010000656.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2255)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2255)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2255
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36669_length_2255_cov_8.210000"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..276)
                     /locus_tag="PLT08_14800"
     CDS             complement(<1..276)
                     /locus_tag="PLT08_14800"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HNE05792.1"
                     /translation="MNLFDRFEQNAHNHPEKTAFIYFDRGLWKTLTYKDLLDKTQRFI
                     RGLEAGHYTPGSTAALLTPPNADFFPFAIALLKLGIVPILVEPALGIK"
     gene            480..731
                     /locus_tag="PLT08_14805"
     CDS             480..731
                     /locus_tag="PLT08_14805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE05793.1"
                     /translation="MKTKPIEQTEAVEDRNAMLEKALIEEYLKEKGYTLEAVKRLPAE
                     LAEKLWKEASQYASLKMEEVEARAHFIKELHDDTSPLTE"
     gene            767..1270
                     /locus_tag="PLT08_14810"
     CDS             767..1270
                     /locus_tag="PLT08_14810"
                     /inference="COORDINATES: protein motif:HMM:TIGR00072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation protease"
                     /protein_id="HNE05794.1"
                     /translation="MKTIVVGLGNPILGDDGVGWKVAEDIQKQLPQFLSQAEGKIDVT
                     RLSLGGLGLMEQLIGYDRAILIDAFETDDQPGSILIMKLNDLPNYTAFHTPSARDVSL
                     QKAIELGRMLGAKLPKEVEVVGITTSKVQDFSEELSPPIADVVSFAARIVLDLLQQIQ
                     IHKEQYA"
     gene            complement(1300..1764)
                     /locus_tag="PLT08_14815"
     CDS             complement(1300..1764)
                     /locus_tag="PLT08_14815"
                     /inference="COORDINATES: protein motif:HMM:NF012792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNE05795.1"
                     /translation="MDAPLLFRRATIQDLPAIVHMLADDDLGSQRERDEDPLPETYLR
                     AFLEIDADKNHELIVAELSGNIVGTLHLTFLPSISYQGGLRSQVESVRVDSKLRSQGI
                     GAKMMQYAIIRAKERGAYLMQLTSHQMRINAHRFYEKLGFKKAHVGMKLILK"
     gene            complement(1764..2057)
                     /locus_tag="PLT08_14820"
     CDS             complement(1764..2057)
                     /locus_tag="PLT08_14820"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF025895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNE05796.1"
                     /translation="MDSEKLEVIHNPTESRFEIRLDGQLAKLDYVEDGDSIVMTHVGV
                     PMEFRGHGIAAIITKAGLDYAKVKSLRVIPMCSYVAAYLRRNPQYIELTRSKE"
     gene            complement(2061..>2255)
                     /locus_tag="PLT08_14825"
     CDS             complement(2061..>2255)
                     /locus_tag="PLT08_14825"
                     /inference="COORDINATES: protein motif:HMM:NF012703.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginase family protein"
                     /protein_id="HNE05797.1"
                     /translation="IPEFGGLTSRDVLTLIKSMQDLSVVGLDVVEIAPSLDPSEATVF
                     AGLKIIMEYIAVMARKKQKG"
BASE COUNT          631 a          453 c          550 g          621 t
ORIGIN      
        1 tttgattccc agtgcaggct cgacgaggat ggggacgatg ccgagcttaa gcaaagcgat
       61 ggcaaaggga aagaagtccg cgttgggggg agttaataaa gcggcggtac tgcctggggt
      121 gtaatgccca gcttcaagtc cacgaatgaa gcgttgggtt ttgtccaata ggtctttgta
      181 ggtgagcgtt ttccagagtc ccctatcaaa ataaataaag gctgtttttt cggggtgatt
      241 atgggcattt tgttcgaagc gatcaaagag attcatggtc aaaattgtat ccctttgtgt
      301 ggatgtttgt cagtgttgta cgattatctt tagtactgtg cttataagag ctggtgttgc
      361 aataataata ttgggaggaa ttcaagactg cgaatggttt atgccgagca ggaaaatgca
      421 tctgttttga acgataggtg catgagggtt cagggttcca aataaaatcg gagcatgaaa
      481 tgaaaaccaa accgatcgaa cagacggaag ccgttgaaga tcgcaacgcg atgcttgaaa
      541 aagccctcat tgaagaatac ttgaaagaaa aaggctatac attggaggca gttaagcgct
      601 tgcctgcgga acttgccgaa aagctttgga aagaagcatc acaatatgct tcattgaaaa
      661 tggaagaggt ggaagctcgc gcacacttca tcaaagaatt acatgacgat acttctcctc
      721 ttacagaata atatacatat ttgttgagtt gggcggtgga ggcatcatga aaacgatcgt
      781 agttgggctg ggtaacccta ttctcggtga tgatggggtt ggatggaaag ttgctgaaga
      841 tattcaaaaa cagcttccac aatttctgtc acaagcagaa ggcaagattg atgtgactcg
      901 cctttctttg ggtggattag ggctgatgga gcagctcatc ggatatgacc gtgcaatctt
      961 gatcgatgct tttgaaactg atgaccagcc cggctcaatc ttgatcatga agttaaatga
     1021 tttgccaaat tacactgctt ttcatacacc cagtgcgcgc gatgtttcgc ttcaaaaggc
     1081 aattgagtta gggagaatgt tgggggcaaa actgcctaaa gaagtcgaag tggttggaat
     1141 caccacctct aaggtgcaag attttagtga agaattatca ccgcccattg ccgatgtggt
     1201 ttcctttgct gcacgcatcg tgctagacct gttacagcaa attcaaatcc acaaggaaca
     1261 gtatgcttaa aataagaact ccggaaaatt ccggagttct tattttaaga tcaacttcat
     1321 gcctacgtga gcttttttaa agcctagttt ttcataaaaa cgatgagcat ttatgcgcat
     1381 ttggtgcgaa gtaagttgca ttagatatgc acctcgctcc ttggcgcgga taatggcata
     1441 ttgcatcatt tttgcgccaa tgccttgact acggagtttt gaatccacac gcactgattc
     1501 tacttgagac cttaatccgc cttggtaact aatggagggc agaaaggtta ggtgaagtgt
     1561 ccctacaata tttccactta attctgcaac gatcagttcg tggtttttat ctgcgtcaat
     1621 ttcaagaaat gctctgagat aggtttcggg aagcgggtct tcatctcttt cacgttgact
     1681 acctagatca tcatccgcca gcatgtggac aatagcaggc aagtcttgta ttgtggcacg
     1741 gcgaaagaga agcggggcgt ccattattct tttgaacggg tcaattcgat gtattgtggg
     1801 ttgcggcgaa gataggctgc cacatacgag cacatgggaa tgacgcgtaa ggactttacc
     1861 ttggcataat ccaaacctgc cttcgtaatg atggcggcaa tgccatgccc acgaaactcc
     1921 atgggtacac caacatgggt catgacaatt gaatcgccat cctctacata atccaatttg
     1981 gcgagttgac catcaagcct aatctcaaag cggctttctg ttgggttatg aatgacttcc
     2041 aatttttcag agtccatgta ttagcctttc tgttttttac gtgccatgac agcgatgtat
     2101 tccatgataa ttttcaaccc ggcaaataca gttgcttcag aagggtcaag tgacggagcg
     2161 atctccacta catccaagcc tacaaccgat aaatcttgca tcgacttaat caacgttaga
     2221 acatcgcgtg aagtaagccc gccgaattca ggaat
//
LOCUS       DAOQJE010000657         2248 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_36880_length_2248_cov_6.758778, whole genome shotgun sequence.
ACCESSION   DAOQJE010000657 DAOQJE010000000
VERSION     DAOQJE010000657.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2248)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2248)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2248
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36880_length_2248_cov_6.758778"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..404)
                     /locus_tag="PLT08_14830"
     CDS             complement(<1..404)
                     /locus_tag="PLT08_14830"
                     /inference="COORDINATES: protein motif:HMM:NF025091.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate lyase/phosphoenolpyruvate mutase
                     family protein"
                     /protein_id="HNE05798.1"
                     /translation="MSQAAKAQQLLSLHNNGKLLILPNVWNPIGARMLQAKGCSAVAT
                     ASAAIAESLGYSDGEQLKLDTMLDMVARIVRSVDLPVTADFEAGYSDSIEGLQENISR
                     LLDTGAVGLNFEDSFDDSAHLRPIPEQVERIKA"
     gene            complement(422..1360)
                     /locus_tag="PLT08_14835"
     CDS             complement(422..1360)
                     /locus_tag="PLT08_14835"
                     /inference="COORDINATES: protein motif:HMM:TIGR01297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HNE05799.1"
                     /translation="MHTHSHTHIGDLTQQTTKRLTLSLVLTAAFVIVEIVAGVFGNSL
                     ALLTDAAHNFTDVIALGLSWYALRIAMKPAHAGKTFGYHRVGILVALINSTTLILIAF
                     GIFFEAYHRFLTPPEVDSTILIGVGAVAFIINLVTALMVKEGSEHDLNMRSAFLHLMG
                     DVMSTLGAVIAGIIIAFTKWNWLDPLVSVLIGAFILYNAWGILKQTIHILLESTPENI
                     NVDNMVLDLQNINGVLGVHDLHVWSISENLRMLSAHVVIGDTTIGEGVSIQQNINELL
                     AHSYNIHHATLQMECEGCTSKDLFCQITEAHQHAHH"
     gene            complement(1364..1654)
                     /locus_tag="PLT08_14840"
     CDS             complement(1364..1654)
                     /locus_tag="PLT08_14840"
                     /inference="COORDINATES: protein motif:HMM:NF033788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HNE05800.1"
                     /translation="MKLPELKAIKLAELFSSLSDASRIRIIASLLDGEMSVRVLAEGL
                     GMTESAVSHQLRGLRQMHLVRARKSGRQVFYSIDDDHVAKLFTLGLDHVQHG"
     gene            complement(1736..>2248)
                     /locus_tag="PLT08_14845"
     CDS             complement(1736..>2248)
                     /locus_tag="PLT08_14845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE05801.1"
                     /translation="YGGNWNETFTGSLNENGEAVFQTEVLGDFTLVLNGENTFKSTST
                     ELSFCGIRGANQELPAGCGFSGKWIVPSKHVFLPGSYMILKQTGENVSGEIFDGNGNP
                     YESFNGTMEWGKGWRANGTYKERGDISLWINASETGFEFVNPKGNIQQLCAVREGVAS
                     AYLGSYSCEP"
BASE COUNT          585 a          501 c          583 g          579 t
ORIGIN      
        1 gctttgatgc gctccacttg ttcagggatg gggcgtaggt gggcgctgtc gtcgaagcta
       61 tcttcaaaat tcaagccgac agccccggta tcgagcaacc gtgaaatatt ttcttgtaaa
      121 ccttctatac tatcggaata ccctgcctca aaatctgcgg tgacgggcag gtctacactg
      181 cggacgatac gagcgaccat atctagcatg gtatccagtt tgagttgttc gccgtcgctg
      241 tagccaagag actcggcaat ggctgcgctg gctgtggcta cagccgagca ccctttggct
      301 tgcaacatcc gtgcgccaat gggattccac acattaggca gaatgagcag tttaccgttg
      361 ttgtgtaagg ataaaagttg ttgtgctttt gcggcttgag acatgggact ccaaataaaa
      421 gttagtggtg ggcgtgctga tgtgcctcag tgatctggca aaacaggtct ttggatgtac
      481 agccttcaca ctccatttgt aaagtggcgt gatggatatt gtaactgtgg gcaagcagtt
      541 cattaatgtt ttgttgaata gagacgcctt cgccaatggt agtatcgccg ataaccacat
      601 gcgctgagag catgcgcaag ttttcactga tgctccacac atgcaggtca tggacgccta
      661 aaacaccgtt gatgttttgc aggtcaagca ccatgttatc tacatttatg ttttcgggag
      721 tgctttctag caagatgtga atggtctgct tcaggatgcc ccaggcattg taaagaataa
      781 acgcgccaat caatacacta acaagtgggt cgagccagtt ccattttgtg aaggcgatga
      841 tgatacctgc aatgaccgcc ccaagtgtgg acatgacatc acccatgaga tgaagaaagg
      901 cgctgcgcat gttgagatca tgttcgctgc cttctttgac cattaatgcg gtgacgaggt
      961 tgatgataaa agccacagcg ccaactccga tcaggatggt cgaatcaact tcgggcggag
     1021 tcaagaagcg gtgataggct tcgaagaaaa tcccaaaggc gataagaatt agcgtcgtcg
     1081 agttaataag cgcgacaaga atcccaacac ggtgatagcc gaaggtcttg ccagcgtgag
     1141 cgggtttcat ggcgatgcgt aaggcatacc aacttaagcc taatgcaatg acatcggtga
     1201 agttatgggc tgcatcggta agcaaagcaa gactgttgcc aaaaacacct gcgacgatct
     1261 caacaatgac aaatgctgca gtgagaacta aggagagggt gaggcgtttg gtggtttgct
     1321 gggtaagatc gccaatatgg gtatgagaat gagtgtgcat gggttatccg tgctgaacgt
     1381 gatcgagacc cagcgtaaat agctttgcca cgtggtcatc gtcgatggaa taaaaaactt
     1441 gtctgccaga tttgcgtgcg cgaacgagat gcatttggcg caacccacgg agctgatgcg
     1501 atacagcgga ttcggtcatg ccaagtcctt cagcaagcac gcggacgctc atttctccat
     1561 ccaagaggga tgcgatgatg cgaatgcgac ttgcatcgct tagggaactg aatagttcgg
     1621 caagtttgat ggctttgagt tcgggtaatt tcataattga atatatgaac aactattcat
     1681 atattactct gtaaaaaaga ccctgtcaat atggtgtttg gcagggtctg agtaattacg
     1741 gttcgcagga ataactgcct agatatgcac ttgctacgcc ttcacgcact gcacaaagtt
     1801 gttgaatgtt acctttcggg tttacaaatt cgaagccggt ttctgaggca tttatccata
     1861 gcgaaatatc gccgcgttcc ttgtaggtgc cattggcgcg ccagccttta ccccattcca
     1921 ttgtgccatt gaaactttcg taggggttgc cattgccgtc aaagatttca ccactcacat
     1981 tttcaccggt ctgcttcaag atcatatagc ttccgggcag aaaaacgtgt tttgaaggga
     2041 caatccactt tccactaaag ccacagccgg caggaagctc ttggttggca ccgcgaattc
     2101 cgcaaaatga taattcggtc gaggtggatt tgaaggtgtt ctctccattg agaacaaggg
     2161 tgaaatcacc taatacttcg gtttgaaaga cggcttcgcc gttttcattg agtgaaccag
     2221 taaaggtctc attccaattg ccgccata
//
LOCUS       DAOQJE010000658         2240 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_37135_length_2240_cov_5.686499, whole genome shotgun sequence.
ACCESSION   DAOQJE010000658 DAOQJE010000000
VERSION     DAOQJE010000658.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2240)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2240)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2240
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37135_length_2240_cov_5.686499"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..397)
                     /locus_tag="PLT08_14850"
     CDS             complement(<1..397)
                     /locus_tag="PLT08_14850"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNE05802.1"
                     /translation="MTVTTYKLDASHLHLFNQFDRTFSVSSKVLLDMTGGRLTWGIIP
                     IEPYEKEIPIDEVDPATFINSEDKVVFLAEVDGKLAGQIKLITWWNGYAYIDDVIVNP
                     QFRGQGVGKALMEAAIQWSREKRFPGIMLE"
     gene            complement(402..1397)
                     /locus_tag="PLT08_14855"
     CDS             complement(402..1397)
                     /locus_tag="PLT08_14855"
                     /inference="COORDINATES: protein motif:HMM:NF013219.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HNE05803.1"
                     /translation="MMKKPFLSSLLFLLFAIVLSLAIGSVFISPTELWKILRGVGEEK
                     FAFIIWNIRLPRTVLIALTGAALSGSGASYQGLFRNPLADPFLIGVASGAGLGAVIAM
                     SIQWPYSFWGLMAIPAAAFISALLTVFIVYFLARVGQTIPTTNLILAGVAFSSFATSL
                     TSFLMLRSTNEVRRALGWLLGGASQAGWTAILAMLPYLVIGLGILIFFGYQLNLLQFG
                     DDQAQQMGLNVNRTKTILLIASSLATAAAVAFSGIIGFIGLVVPHIMRLWFGADYRRL
                     IPLSIISGASALLFSDIIARIVLAPQEIPVGIITALAGAPFFLWVLRRVKNQGYW"
     gene            complement(1498..>2240)
                     /locus_tag="PLT08_14860"
     CDS             complement(1498..>2240)
                     /locus_tag="PLT08_14860"
                     /inference="COORDINATES: protein motif:HMM:NF013648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="helical backbone metal receptor"
                     /protein_id="HNE05804.1"
                     /translation="EILFAVGAGDQVIGRDEFSDYPAEAAAIESVGGSMGEYSAEAIV
                     ALEPDLVLAAEINTPELISQLEDLGLTVYYLSNPTTIEEMYTNLEIVGQLTGHDTTEM
                     VDSLKARVAAVDEKIAPISSRPSVFYEIDASDPSKPWTYGPGSFGNLLIERAGGFNIA
                     SDAPDSYPQLSLEQVVVANPSFILLGDSMWGVTPESVLDREGWNTILAVQNNQIFPID
                     DNLISRPGPRLVDGLEALAKILHPGLFE"
BASE COUNT          620 a          525 c          590 g          505 t
ORIGIN      
        1 tttcgagcat gatgccggga aaacgcttct ctcttgacca ttgaatggca gcttccatca
       61 gagcttttcc tacgccttga cctctgaact gcgggttgac gatcacatca tcaatatagg
      121 catatccgtt ccaccatgtg atgagcttga tttgcccggc aagtttgcca tccacttccg
      181 caaggaagac cactttatct tcactgttga taaaagttgc agggtcaacc tcatcgatgg
      241 ggatttcttt ttcatatggt tctataggta tgatgcccca ggttaatctg ccaccggtca
      301 tatctagtaa gacctttgat gagacggaaa aggttcgatc aaattggttg aaaagatgca
      361 ggtgagatgc atcaagttta tatgtagtga ctgtcatgtc tttaccaata cccttgattt
      421 ttcacgcggc ggagaaccca taaaaagaaa ggtgcgccag ccaatgcagt gatgatgccc
      481 accggaatct cttgcggagc aagcacaatg cgggcgatga tatctgaaaa taaaagcgca
      541 gatgcgccgc tgataataga gagcggaatg agtcttcgat aatctgcgcc gaaccacagg
      601 cgcataatat gtggcaccac cagaccgata aagccgatga tgccagagaa tgccactgct
      661 gctgcggttg caagcgacga tgcgattagc aaaatagttt ttgtgcggtt gacgttcagc
      721 cccatttgct gagcttggtc atcgccgaat tgcaaaaggt tgagttggta cccgaagaaa
      781 atcagaatcc ccagaccaat gacaagatat ggcagcatgg cgaggatagc cgtccacccg
      841 gcttgactag ctcctccaag caaccatccc agtgcgcggc gaacttcgtt ggtggagcgc
      901 agcattagaa aggaggtgag tgatgtagcg aaagaagaga aggcaacccc ggctagaatc
      961 aaatttgtgg tgggaatggt ttgaccaacc cgggcaagaa aataaacaat aaatactgta
     1021 agcaatgccg agataaatgc agcggcagga attgccatga gcccccaaaa cgaatacggc
     1081 cactgaatag acatggcaat caccgccccc agccctgcgc cagatgccac gccgatcagg
     1141 aatgggtctg cgagcgggtt acgaaataac ccttggtatg atgcgccgct tccgcttagg
     1201 gcggcaccgg taagagcgat tagcactgta cgtggtaagc ggatgttcca aatgatgaag
     1261 gcaaattttt cttctccaac accgcgcaga atcttccata actccgttgg ggaaatgaaa
     1321 accgagccaa tggcgagact gagcacaatg gcgaataaaa ggaaaagcaa agaagaaaga
     1381 aaaggttttt tcatcataac gagtgcatcg caccaaagga taggataagt ttaccgtgta
     1441 gttttgctga tgtaaggaga cttatctacc catttggtgc gacacacgtt tggtcaatta
     1501 ctcaaataga ccggggtgca aaatctttgc caatgcttca agcccatcta ccaggcgcgg
     1561 accggggcgg ctgatgaggt tatcgtcgat ggggaagatc tggttgtttt gaacggcgag
     1621 aatggtattc cagccttcac gatccaacac ggattcggga gtgacgcccc acatagaatc
     1681 accaagcaaa atgaaagaag gattcgcgac gacaacttgt tccaaactta attgtggata
     1741 ggagtctggc gcgtcgctgg cgatgttaaa tccaccggcg cgttcgatca gtaaattacc
     1801 aaatgaaccg ggtccatacg tccaaggttt ggaggggtca gaggcgtcga tttcgtagaa
     1861 gacggatggg cgtgagctaa taggtgcgat cttctcatcc acggcagcaa cgcgagcctt
     1921 gagcgaatcg accatttcag tggtgtcgtg cccggtgagt tgaccgacga tctcaagatt
     1981 ggtgtacatt tcttcaatcg tggtggggtt gctcaaataa taaacggtga gccccaaatc
     2041 ttccagctgg ctgatgagtt cgggggtgtt gatttcagcg gcgagcacca gatcaggttc
     2101 gagggcaacg atggcttcgg cgctatattc acccatggag ccgccaacac tttcaatggc
     2161 agccgcttca gcggggtaat cagaaaactc atcgcgacca atcacctgat caccggcgcc
     2221 gacggcaaaa agaatttctg
//
LOCUS       DAOQJE010000659         2238 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_37191_length_2238_cov_7.579020, whole genome shotgun sequence.
ACCESSION   DAOQJE010000659 DAOQJE010000000
VERSION     DAOQJE010000659.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2238)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2238)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2238
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37191_length_2238_cov_7.579020"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1235)
                     /locus_tag="PLT08_14865"
     CDS             complement(<1..1235)
                     /locus_tag="PLT08_14865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HNE05805.1"
                     /translation="MALEKQPGAFSDTAWHALEAEQVMRDLEVHDEGLTTAEAEKRLA
                     HYGQNQLQEAPRPGFLAMLWDQLNNFVVILLIVASVISALLGDYVEAIAIMAIVVLNA
                     ILGIIQEQRAEQALAALKKLAAPEAQVMRDGSRKSVPAYNLVPGDIVFLEAGNFIPAD
                     LRLLEAVNLRVEEASLTGESLPVQKNASTLLDKNVPLGDRKNTAFMGTVVSYGRGRGV
                     VTSTGMNTQLGLIATMLQSVESEETPLQRRLDQLGKSLSIGSLILVFVVFIVALFNYT
                     EVSGLFSDPINYFKTFAEQITEVFIIAISLAIAAVPEGLPAVVTISLALGMREMIQRH
                     ALIRKLSSVETLGSATTICSDKTGTLTQNEMTVTRLWADGQFVSVSGTGYTPKGDFLV
                     DGKVVDAKQYPAILSTLWLG"
     gene            1507..1947
                     /locus_tag="PLT08_14870"
     CDS             1507..1947
                     /locus_tag="PLT08_14870"
                     /inference="COORDINATES: protein motif:HMM:NF012792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNE05806.1"
                     /translation="MPVIKIPKSRDDFKAYYALRYKVLREPWGHPKGTEKDDFEAISE
                     HFMAVAEKTGEILGVIKLFEKEAGVGQVSHLAVATEHQHKGVGKFLVEAVEARAREHG
                     FKTLGAMVRVTATAFFEKHGYHIAGLPVPHLGTTHLVWMEKPLA"
     gene            complement(1994..>2238)
                     /locus_tag="PLT08_14875"
     CDS             complement(1994..>2238)
                     /locus_tag="PLT08_14875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE05807.1"
                     /translation="VWKIEIPDLLRFGISVATIVLTLAYAHIPFLQRFRFVLLWFAQG
                     IGIINKLVDWLFMPIKGIFSLIAGLFSRPSNSQPAE"
BASE COUNT          645 a          510 c          545 g          538 t
ORIGIN      
        1 ccaagccaca gtgtggaaag aatggcggga tattgctttg catccaccac tttgccgtct
       61 acgagaaaat cacctttggg cgtgtatccg gttcctgaaa cgctgacaaa ctgcccgtct
      121 gcccagaggc gcgtgaccgt catctcgttt tgggtgagag taccggtttt atccgaacaa
      181 atcgtcgttg ctgacccaag cgtctccact gaagagagct tgcggataag ggcatgacgt
      241 tgaatcattt cgcgcatgcc gagtgccagt gagatggtta caacagcggg cagaccttcg
      301 ggaacggcag caattgcgag gctgatagcg atgatgaaga cttcggtaat ttgttcggcg
      361 aaggttttga aataattgat ggggtcagaa aatagaccgc tgacttcggt gtaattgaaa
      421 agcgcaacaa taaaaacgac aaagacaaga atcaacgaac caatactgag tgattttcct
      481 aattggtcga gccggcgctg aagcggagtc tcttcggact ctacactttg caacatggtg
      541 gcgatgagac ccaattgggt attcataccc gtgctagtga ctacaccgcg cccacgtcca
      601 tatgaaacga ccgttcccat aaacgctgtg ttcttgcgat ctcccaacgg gacgtttttg
      661 tcgagcaatg tagatgcatt cttttgtaca ggaagtgact cgcctgtaag tgaagcttct
      721 tccacacgca agttgaccgc ttccagcaag cgcaaatcag cagggataaa gttcccggct
      781 tcaagaaaaa caatatcacc aggcacaagg ttataggctg ggacagattt ccgcgagcca
      841 tcgcgcatca cttgcgcttc gggtgcggca agcttcttga gggcggcaag agcttgttcg
      901 gcacgttgct cttgaataat accgagaatt gcattcaaga ccacaattgc catgatggca
      961 attgcttcaa cataatcacc cagcaaagca gaaataaccg aagcaacgat caatagaata
     1021 acaacgaaat tgtttaactg atcccaaagc atagcaagaa agcctgggcg gggcgcttct
     1081 tgtagctggt tctgaccgta atgcgcaagc cgcttttcag cttctgcggt ggtcagacct
     1141 tcatcatgca cttccagatc acgcattacc tgttctgctt ctaaagcatg ccaggcggta
     1201 tctgaaaacg caccgggttg tttttctaat gccatctatg gtctcctgat aaaagataaa
     1261 aaagaccacc acgaatcaaa acatcgtggt ggtcttgcca acgcaatttg cgtatggatc
     1321 accgatggcg tttaaccaat ggtctgacga tgatccatcc tgtggggaac aggcggctac
     1381 tccccaacca aaagagagtg tactccactg caaaagtcac tgtcaaggaa aagggtctac
     1441 ggttttgtat ggcattctca agagaagtat aatgaaactg aacaatggtt catttgtgga
     1501 gggaacatgc ctgttatcaa aataccaaaa tcacgagatg acttcaaagc gtattatgcg
     1561 ttgcgctata aagtattacg cgaaccatgg ggtcacccca aaggcacaga aaaagatgac
     1621 tttgaagcca ttagcgagca ttttatggca gtggctgaaa agaccgggga aattctgggt
     1681 gttatcaagc tattcgaaaa agaagctggg gtagggcaag tttcacactt ggcagtcgct
     1741 acggaacatc agcacaaagg ggttggaaaa ttccttgttg aagccgttga ggcgcgagct
     1801 cgtgaacatg ggtttaagac tcttggcgca atggtgcgtg tgaccgccac agcttttttt
     1861 gaaaaacatg gctatcatat tgcagggttg cctgttccac atctagggac aacccatctg
     1921 gtttggatgg aaaaaccact ggcatgaatc aaaaaaggat aagcattttt gctcatcctt
     1981 tttttctgta aaattattct gctggttgcg aatttgatgg acgtgaaaaa agacccgcga
     2041 ttaaactaaa gatgcctttg atcggcataa agagccaatc aaccagtttg ttgataattc
     2101 caattccctg agcgaaccac agcaaaacaa agcggaagcg ctgtaagaaa gggatatgtg
     2161 catacgccag tgtcaataca atggtcgcaa ctgaaatccc aaaccgcagc aaatcaggga
     2221 tttcaatttt ccacacct
//
LOCUS       DAOQJE010000660         2238 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_37193_length_2238_cov_4.947778, whole genome shotgun sequence.
ACCESSION   DAOQJE010000660 DAOQJE010000000
VERSION     DAOQJE010000660.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2238)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2238)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2238
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37193_length_2238_cov_4.947778"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..219
                     /locus_tag="PLT08_14880"
     CDS             <1..219
                     /locus_tag="PLT08_14880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE05808.1"
                     /translation="GYVQKEMRLALDRALEMPQGEIFLVPARFEEVDLPYSLRDYQWV
                     DLYTENGKTKLLKSLALRAQKVGAKLPI"
     gene            complement(279..1598)
                     /locus_tag="PLT08_14885"
     CDS             complement(279..1598)
                     /locus_tag="PLT08_14885"
                     /inference="COORDINATES: protein motif:HMM:NF004426.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl ornithine aminotransferase family
                     protein"
                     /protein_id="HNE05809.1"
                     /translation="MANINLPGPKAKAMIERDHKVISPSYPRGYPFAMDHGKGTEVWD
                     VDGNRFLDFMGGIAVVATGHSHPQVVKAIQEQAEKFIHISSDFYHENWIKLGEKLNEI
                     APFEEDALSFMTNSGTEAVEAAIKLARYHTKRSNFIGFTGAFHGRTMGAVTFTASKSK
                     YHSGFYPLMNGVTHAPYPNPYRPVLERRKGEDYGEAVVRYIEEEIIAQILPAKEVAGV
                     LVETIQGEGGYIVPPDGFYPALRKFCDKHGILLIVDEVQSGMGRTGKWWAIEHFGVEP
                     DIVTSAKGIASGMPLGACIARKSVMDWEIGTHGNTYGGNPISCAASLATIDLIQKQYL
                     QNSKEVGEYAMDALMEIQSRHPSIGEVRGKGLMIGVEFVKDRETKEPAKHLTERVVDI
                     AYERGLLMLSCGQSVIRIAPPLSISKSEVDEGLRMFEEAITQAEKEK"
     gene            1952..>2238
                     /locus_tag="PLT08_14890"
     CDS             1952..>2238
                     /locus_tag="PLT08_14890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE05810.1"
                     /translation="MLYFFNRFIEILRDIPRWLRPGLGVKRWLLFVMAGITLIGLGIA
                     VVLIDIYRTNTTDPVVLTFLSYASLRFLPRLLRAIVFGGLGVALIVYGMYK"
BASE COUNT          603 a          482 c          464 g          689 t
ORIGIN      
        1 ggatatgtcc aaaaagagat gcgcctcgca ctagaccggg ctctggagat gcctcaagga
       61 gagattttcc ttgtcccagc tcgttttgaa gaagttgatc ttccatatag tctgcgcgat
      121 tatcaatggg tagatttata tacagagaac ggtaagacaa agttgttgaa aagtttagca
      181 ttgcgtgcgc aaaaggttgg agctaaactt cctatatagt tttgccataa aaaagagacc
      241 ctcaaattat ttgagggtct ctttttttat ggcaatggct atttttcttt ttctgcttgt
      301 gtaattgctt cttcaaacat tcgtaaccct tcatctactt cactcttgct tatagaaagt
      361 ggaggtgcaa tacgaataac gctttgtcca caagatagca tcaacaatcc gcgttcgtaa
      421 gcgatgtcaa caaccctttc tgttaagtgt tttgccggct ctttggtttc acgatctttt
      481 acgaattcga ccccgatcat taaacccttc ccacgaactt cgccgatgct ggggtgtcta
      541 gattggattt ccattaaggc atccatggca tattcaccaa cttctttgga gttctgcaaa
      601 tactgttttt ggatcaagtc aattgtcgcg agagaggcgg cacaagaaat agggttgcca
      661 ccataggtat tcccgtgtgt accgatttcc caatccatta cagattttcg tgcaatacat
      721 gcaccaagcg gcatgcctga agcgatccct tttgctgaag taacaatatc tggctccaca
      781 ccaaaatgct caattgccca ccatttacca gtgcggccca taccagattg cacttcatct
      841 acaattagca aaataccgtg tttgtcgcag aattttcgta gtgccgggta aaaaccatct
      901 ggcggaacaa tataaccgcc ttcgccttga atcgtctcaa ctaatacacc agcaacttct
      961 ttggctggca aaatttgcgc aatgatttct tcttcaatgt aacgaaccac tgcctcacca
     1021 taatcttcac ctttgcggcg ctctaagacg gggcgataag gattagggta gggagcatgt
     1081 gttacgccat tcatgagcgg ataaaatcca ctatggtatt ttgatttgct agcagtaaaa
     1141 gtgacagcac ccatggttcg accatgaaat gcgccggtaa agccaatgaa gtttgaccgt
     1201 ttcgtatgat aacgagcaag tttgattgct gcttctactg cttcagtacc ggagttggtc
     1261 ataaatgaga gcgcatcttc ttcaaaaggc gcgatttcat ttagtttttc accaagtttg
     1321 atccagttct cgtgataaaa gtcagaagag atgtgaatga acttttctgc ctgttcctga
     1381 atggctttga caacctgcgg atgagaatga cccgttgcga ccacagcaat accacccata
     1441 aaatctagga agcgattgcc atctacatcc caaacttcgg tgcctttgcc atgatccatc
     1501 gcgaacggat atccacgtgg ataggaagga gagatcactt tgtgatcccg ttcgatcatt
     1561 gcctttgcct ttggaccggg aagatttata tttgccataa ctactccttg gaatgaattg
     1621 aaaaaccaga ttttgtttaa actggcatct gtatgccagg taaatctgca ttagcatcga
     1681 attgagcttc cgtaattatc aatgcaacta aacaatataa gttgatattg catctaaacc
     1741 gataaaaaag catcttacaa agaaactaag ataggggaag tagtgactat tgagaaaata
     1801 aatcttgcgt atagacaacg ctggtattta cttgccactg tcataactgg caaaaattga
     1861 tgacatactg tgattatacc gtaaggcggt ttccctttat ttttggcaac aggtttgacg
     1921 tcaaaggtca atcctgctat tatcgattct tatgctttac ttttttaatc gttttataga
     1981 aattctacgc gatattccac gctggcttcg accagggtta ggggttaaac gctggctttt
     2041 attcgtaatg gctggtatca cgttgattgg cttaggcatt gctgttgttt taattgatat
     2101 ctaccgcaca aatactacag accctgttgt gctcacgttt ttatcctatg cttccttgcg
     2161 gtttttacct cgtcttttac gtgccatcgt ttttggtgga cttggcgtgg cattaattgt
     2221 ttatgggatg tataagat
//
LOCUS       DAOQJE010000661         2223 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_37680_length_2223_cov_2.832103, whole genome shotgun sequence.
ACCESSION   DAOQJE010000661 DAOQJE010000000
VERSION     DAOQJE010000661.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2223)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2223)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2223
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37680_length_2223_cov_2.832103"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(214..1812)
                     /locus_tag="PLT08_14895"
     CDS             complement(214..1812)
                     /locus_tag="PLT08_14895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE05811.1"
                     /translation="MKIKLSITQFILLIALFTVLTLTVLKQSNPGTELPNRDYGFYSY
                     IGLQIVRGELPYKNVWESKPPAIFYLNAFALRIGRGLRWGVWFVEFSFLFSAIAASFN
                     LLKKLWGITSAIFGIFVWLWGMNFTLQGGNYTEEYPLVFHFLALTLLLHLIQRPKQRL
                     INLSLGLLFSISFLFRPNNAIVEAVTILVLGASFLWKRDWQAFFTTVFWVTLGIVIPL
                     IITSAYFAYHGLFKEMLEASILYNLTYSTTDLSTSSPLKVGFEFLNLAAWIGLVGYLL
                     LFVKIKDILKNNNLVFFIVLLIGTPIAVYFSDPAKRNYGHYFMNWLPFIGVLSAFAFH
                     TFQEKFISIKKQQLEINLQNILSFPPSTAFYFLIAIILFISTGCIPIYFKAFDRLLTS
                     SEREIRSPIAIYVNNHTQPGDYVLFWATHPGENFMSRRDTPVTALFYPNMVNTSEIAQ
                     RLNDDFLNDLQNNPPVLIVDMGRLTIPSLDPAKRQEQKSMGVYPTDPPDNLDKVLTFI
                     SKNYHLENIIKDKPVYRLNGTIVP"
     gene            complement(1849..>2223)
                     /locus_tag="PLT08_14900"
     CDS             complement(1849..>2223)
                     /locus_tag="PLT08_14900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE05812.1"
                     /translation="FMTDAVCAPTPGNPSAIDETKYAKNRCGSRSSSSSESQQHLNSL
                     SLTASINSDVLPYPAGAETSTSLLSSASNNWFNNRLRVNNSARLRGGIILVLLMGMEN
                     MASPFNNKKAATLFYFISESAF"
BASE COUNT          667 a          387 c          557 g          612 t
ORIGIN      
        1 tgttcatttc tttgacgcgt ttgaccagct tcttgatgtt cgagaagccg tcgagtaggg
       61 agtattcggt atgaacgtgg agatgggcga aggacatggg ctagttagat agtgtgcaga
      121 tatttcattt actttggcat tatagcacgc atgttcagac tattccccta cgcgtggctt
      181 aaagttttcg gggtatttag aaatagctaa gctttacggc actattgttc cgttaaggcg
      241 atatacgggt ttatctttga tgatgttttc gagatggtaa ttctttgaga tgaatgtcaa
      301 cactttatcg agattatccg gtgggtcggt gggatagacg cccattgatt tttgttcttg
      361 gcgtttggct gggtcgagcg atgggatggt gagacgtccc atgtctacga tgaggacggg
      421 aggattgttt tgtagatcat tcaggaaatc atcgttgagg cgttgggcga tctcggaggt
      481 gttgaccata ttcgggtaga acagcgccgt tacaggcgta tcgcgacggg acatgaagtt
      541 ctcgccaggg tgagtcgccc agaataaaac atagtcacca ggctgagtgt ggttgttcac
      601 ataaatagcg attggtgaac gaatttctcg ctcggaagag gttaatagac ggtcgaaagc
      661 tttgaagtag atggggatgc acccagtgga tatgaagagg atgatggcaa tgagaaaata
      721 aaatgctgta gaaggcggaa aagatagaat gttctgaaga tttatttcta gctgttgttt
      781 tttgatggat ataaactttt cttggaatgt atgaaaagca aatgctgaca aaacaccaat
      841 aaatggcaac caattcataa aataatggcc gtaattgcgt ttggcaggat cactgaaata
      901 aacagcaata ggagtgccaa tgagtagtac gatgaagaat actaagttgt tattcttaag
      961 aatatctttg atctttacga aaagtaacaa atagcctaca agacctatcc aagcggcaag
     1021 attgaggaat tcaaagccaa ctttcaatgg agaggaggtg cttagatcgg ttgtgctgta
     1081 ggtcaggtta tataaaatcg acgcctcaag catttctttg aaaagaccat gatatgcaaa
     1141 ataagcagac gtgatgataa gcgggatgac aatgcctaat gtaacccaga agactgtagt
     1201 gaagaaagct tgccaatcac gcttccacag aaatgaagca cctaagacga ggattgtgac
     1261 agcttcgaca atggcattgt ttggacgaaa taaaaagcta atggagaaga gcaagccaag
     1321 gcttaggtta atgaggcgct gttttgggcg ttgaattaag tgtaggagga gagtcaatgc
     1381 gaggaagtga aatactaatg gatattcttc ggtgtagttg cctccttgca aggtgaagtt
     1441 cataccccat agccatacaa aaatgccaaa gatggcagat gtaatgcccc acagtttttt
     1501 gagtaaatta aatgaagccg ctatagcaga aaatagaaag ctaaattcta caaaccagac
     1561 tccccaacgc agtccgcgcc cgatgcgtaa agcaaaggca ttaaggtaaa aaattgcagg
     1621 tggtttgctt tcccaaacat ttttataggg taattcgccg cgcacaattt gcagaccaat
     1681 ataagaataa aacccataat cacggtttgg tagttctgta ccagggtttg attgctttag
     1741 aactgtaagc gtgagaactg taaataatgc aatcagcaaa ataaattgtg ttatggataa
     1801 ttttattttc atgggtttca caaattataa cattcagctt tttacacctt aaaaggcact
     1861 ttcagagata aaataaaata aagttgccgc cttcttattg ttgaacgggc ttgccatgtt
     1921 ttccattccc attagtaaaa ccaaaattat cccaccgcgc aggcgtgcgg agttgttgac
     1981 gcgcaagcga ttgttaaacc agttgtttga ggcactggat aacaaacttg tgctagtctc
     2041 agccccagca gggtatggca aaacgtcgct gttgattgat gccgttaaag aaagcgaatt
     2101 caaatgctgc tggctttcac tcgatgaact agatcgagat ccacagcgat ttttggcata
     2161 tttggtttcg tcgattgcag aggggtttcc gggtgtgggc gcacaaacgg cgtctgtcat
     2221 gaa
//
LOCUS       DAOQJE010000662         2222 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_37693_length_2222_cov_7.077065, whole genome shotgun sequence.
ACCESSION   DAOQJE010000662 DAOQJE010000000
VERSION     DAOQJE010000662.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2222)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2222)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2222
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37693_length_2222_cov_7.077065"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..323
                     /locus_tag="PLT08_14905"
     CDS             <1..323
                     /locus_tag="PLT08_14905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE05813.1"
                     /translation="ASWGMFKNLSFAALVQAGLMIQSENRAFSILEEHFGKTHPAWTR
                     AFRLAFGMDVETNVPAYQTRGKASLALYEETAKLFKDIINDKHREVIENTLQLINNFN
                     RDQK"
     gene            351..1016
                     /gene="nth"
                     /locus_tag="PLT08_14910"
     CDS             351..1016
                     /gene="nth"
                     /locus_tag="PLT08_14910"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: protein motif:HMM:NF012933.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0140078 - class I DNA-(apurinic or
                     apyrimidinic site) endonuclease activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HNE05814.1"
                     /translation="MTDLKQRALQIHETLLKVFGEPIWRNPLPAIDELVSTILSQNTN
                     DVNRDRAFEALRAKFPTWEAVRDAKEKDVVEAIKPAGLANQKGPRIQQVLKAITEERG
                     ELNLQFLADMPIEDARNWLTKFNGVGPKTAAIVLCFSLNRPAFPVDTHVYRVTGRIGL
                     RPEKMTVEQAHPHLESIFPPETYYAAHLNIIRLGREVCNARKPMCPQCPVKHLCDFKE
                     KTQ"
     gene            1070..1537
                     /locus_tag="PLT08_14915"
     CDS             1070..1537
                     /locus_tag="PLT08_14915"
                     /inference="COORDINATES: protein motif:HMM:NF015999.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaK/EbsC family protein"
                     /protein_id="HNE05815.1"
                     /translation="METPPVSLALEKLNIPHQIFRHETPVDSFEKAASDRGQRPSQIV
                     RTILFRVSEEEFALVLVAGPGQISWKLLRKLLGKSRITMATEEEVISVTGYRPGTVGP
                     FGLLKPARIMVEAGLLKEEEVSIGSGMRDTAVILKTNDLMKALGNVEVVELFE"
     gene            1573..>2222
                     /locus_tag="PLT08_14920"
     CDS             1573..>2222
                     /locus_tag="PLT08_14920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE05816.1"
                     /translation="MSYKQRTIDFLEIEWATYIERFKRLPELDGVQRVRNQGYERFRD
                     MLAHVLAWWEDVMPVIMAIAENREFEKKKYDFDAFNAEAVAKYKDWDEAEFLAHFEKT
                     RQKAVADLKSMNEAAFEDRRIQGRINGIFIHHAREHLVALSKFLTLDTLEHEWGTYIA
                     RFDASEKKEEFLKKQSAARFGDLLAHNFAWWQEGVNAVQGALKDSSFVYKGPADTDA"
BASE COUNT          609 a          532 c          550 g          531 t
ORIGIN      
        1 atgcttcgtg gggcatgttc aaaaatttat cctttgcggc gttggtgcaa gccggtttaa
       61 tgatccaaag cgagaaccgc gccttttcca tccttgaaga acattttgga aaaactcacc
      121 ctgcatggac gcgcgccttt cgcctcgcct ttggcatgga tgtggaaaca aatgtcccgg
      181 cgtatcaaac gcgcggcaaa gcatccttgg cactctacga agaaacagca aaattgttca
      241 aagacatcat caacgacaag catcgtgaag tcattgagaa tacgctacaa ttgattaata
      301 atttcaaccg cgaccagaaa taaaacagct gctttctttt aataaacctc atgacagacc
      361 tcaaacaacg tgctctacaa atccatgaaa ctctgctaaa ggtctttggc gagcccattt
      421 ggcgcaatcc tctgccagct attgacgaac tcgtctccac catcctttcg cagaatacca
      481 atgatgtaaa ccgtgaccgt gcctttgagg ctttgcgtgc taaattcccc acatgggaag
      541 ccgtgcgcga tgccaaagaa aaagatgtgg tcgaagccat caaacctgcc gggctggcga
      601 atcaaaaagg tccgcgcata cagcaggtgt tgaaagccat taccgaagaa cgtggtgaac
      661 tcaacctgca atttctcgcg gacatgccca ttgaagatgc ccgcaactgg ctgaccaaat
      721 tcaacggcgt gggtcccaaa actgctgcca tcgttttatg cttttctctc aatcgccccg
      781 ccttcccggt ggatacccat gtctatcgcg tgacaggtcg cattggactg cgccccgaaa
      841 agatgaccgt ggagcaagca cacccccatc ttgaatccat cttcccgcca gagacctact
      901 acgccgctca cctgaacatc atccgtttgg gacgggaggt ctgcaatgcg cgcaagccga
      961 tgtgcccgca atgcccggtc aaacatctat gtgatttcaa agagaaaact caataggact
     1021 acactgcgct gttatggcgc agtttttatt ttcagttaga atgcatgcca tggaaacacc
     1081 tcccgtcagc cttgctttag aaaaactgaa tatccctcat caaatcttca ggcatgaaac
     1141 gccggtggat tcgttcgaga aagccgccag tgaccgtggt cagcgccctt cgcaaattgt
     1201 gcgaacgatt ctcttccgtg tgagtgaaga ggaatttgcg ctcgtcctcg tagcaggacc
     1261 gggtcaaatt tcgtggaagt tgctgcgaaa attactcggc aaatcacgca ttaccatggc
     1321 aaccgaagaa gaagtgatat ccgtcaccgg ctatcgccca gggacagtgg gtccgtttgg
     1381 gttgttgaag cccgcccgca tcatggtgga agcgggtctt ttgaaggaag aggaagtatc
     1441 cattgggtcg ggcatgcgcg acacagccgt catcctcaaa acgaatgact taatgaaagc
     1501 gttaggaaat gtggaagtgg tagaactgtt tgagtaaaat ctaactagca tcttatgatc
     1561 tggagagtat caatgagcta taagcaacgc acaatcgact ttttagaaat tgaatgggca
     1621 acgtatattg aacgctttaa acgtcttcct gaattggatg gcgtacagcg tgttcgcaac
     1681 caaggctatg agcgcttccg cgatatgttg gcgcacgtct tggcttggtg ggaagatgtg
     1741 atgccagtga tcatggcaat tgcagagaac cgtgagtttg aaaagaagaa atatgatttc
     1801 gatgccttca atgccgaagc cgtggcaaag tataaagatt gggatgaagc cgagtttttg
     1861 gctcactttg agaaaacgcg tcagaaagcc gtagcagacc taaagtctat gaatgaggcg
     1921 gcgtttgaag atcgtcgcat tcaggggcgc atcaacggca tcttcatcca ccatgcccgc
     1981 gaacatctgg tggcattgag caaattcctc acgctcgata cgcttgaaca tgaatgggga
     2041 acttatattg cgagattcga tgcttcagag aagaaagaag aatttttgaa gaaacaaagt
     2101 gcagctcgtt ttggcgacct gttggcacat aattttgcat ggtggcaaga aggcgtgaat
     2161 gcggtgcaag gtgctttgaa agattcaagc tttgtgtata aaggtccggc agatactgat
     2221 gc
//
LOCUS       DAOQJE010000663         2222 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_37696_length_2222_cov_5.469312, whole genome shotgun sequence.
ACCESSION   DAOQJE010000663 DAOQJE010000000
VERSION     DAOQJE010000663.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2222)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2222)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2222
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37696_length_2222_cov_5.469312"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..779
                     /locus_tag="PLT08_14925"
     CDS             <1..779
                     /locus_tag="PLT08_14925"
                     /inference="COORDINATES: protein motif:HMM:NF017946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC-2 family transporter protein"
                     /protein_id="HNE05817.1"
                     /translation="IKLAFNYLRIGIMNEFQYRANLYIQILQTFIALGTGLIGLNLVF
                     AQTSQLGGWGKADLLAVMGVFTMTGGFIRAAIQPNMERLMDEIREGTLDYALTKPADS
                     QLLVSVREFRLWQLVDVITGAIVLTVALTELKELINVWTVLGFFSALVMGAIMVYSFW
                     LIITSTAFWFVRVNEIANLFEGLYAAGRWPVDIYPNWLRYGLTFLVPVAFAVTVPASA
                     ITGRLTLQTWLLALAVTSILFILARTIWTIGVRNYSGASA"
     gene            848..1549
                     /locus_tag="PLT08_14930"
     CDS             848..1549
                     /locus_tag="PLT08_14930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE05818.1"
                     /translation="MKHRIFQKRFTWACVAFLLIGCSPATTIVPESTATPYSTFTPID
                     TPTNTLTPTPYPSATIVKIPTWDPNQPTFTPFAQFSNIFPTGTSSKPGPGFASVTYSP
                     TKIYWGGCTPNSVTIIAKVDDVEEVTSVLIFVRVKDLTEEDYTPWTNGDVMLNRGQGE
                     FTTVLYGSKIKGHDHYLRSWVYFQLVATNIEGEEIGRTKIYDKAFDMYPCPCLTPLTG
                     CPIATPKPPSPTPKK"
     gene            1553..>2222
                     /locus_tag="PLT08_14935"
     CDS             1553..>2222
                     /locus_tag="PLT08_14935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE05819.1"
                     /translation="MRKIFLLTLLLIFSLACSLGTFAPQPTATPAPTQTDTATPLPTP
                     TPVTPTLTFTSTPTLISNTAIPTIETTSTELSTPTATQALSTPYTVMPPVNIKGFISI
                     TISLTEVFKAKGCEPSVVRITAQVVNPNEVSFVLLFSRFKSLKAERTGKWTTVQMIPL
                     GAGTYMFDLSSAIMKDDAYFENAWIEYQVVSTNQKGTELGRTDIFKERLKMVQCIFTA
                     TPTSA"
BASE COUNT          573 a          574 c          451 g          624 t
ORIGIN      
        1 gaataaaact tgccttcaac tacctgcgaa ttggcatcat gaatgagttt caataccgcg
       61 ccaatcttta tattcagatt cttcagacct ttatcgcctt gggcacgggg ttgattggtt
      121 tgaatttggt cttcgcgcaa acgtctcaac tcggcggttg gggcaaagcg gacctgttgg
      181 ctgttatggg cgttttcact atgacaggcg gatttatccg cgctgccatt cagcctaata
      241 tggagcgcct catggatgag atccgcgaag ggacactcga ctatgccctc accaaacctg
      301 ccgattcgca gttactcgtc agtgtgcgcg agtttcgcct ttggcaattg gtcgatgtga
      361 ttacaggcgc catcgtgttg actgtggcac tcacagaact caaagagctg attaatgtat
      421 ggaccgtgct aggctttttc tccgcgcttg ttatgggtgc gatcatggtt tacagttttt
      481 ggcttatcat cacatccact gctttttggt ttgtgcgtgt aaatgaaatt gctaaccttt
      541 tcgaaggtct atatgccgcc ggacgctggc ccgtggatat ctaccctaac tggctacgct
      601 acggcttgac ctttctcgtc cctgtcgcct ttgctgtgac tgtgcccgct tccgctatta
      661 cgggacgatt gaccctgcaa acctggctcc ttgctttggc tgtaaccagc atcctcttca
      721 tcctcgccag aacgatatgg actatcggcg tacggaacta ctccggcgct tctgcctaac
      781 aaaaaggatg gttactttca aagtaaccat cctttttaca cattagattt catagtatta
      841 tagcgggatg aaacatcgta tttttcaaaa gagattcact tgggcttgtg tggcattttt
      901 gttaattggc tgttctccag caacgactat cgtcccagaa tctacagcta cgccttatag
      961 cactttcact ccaattgata cccctacaaa tacgcttacc cctactcctt acccttcagc
     1021 gaccattgta aaaattccaa cttgggatcc aaatcagcca acctttacac cgttcgctca
     1081 gttttctaat atcttcccaa caggaacatc ttcgaaacca ggtcctggct tcgcttccgt
     1141 tacttattca ccaacaaaga tttactgggg aggttgcact ccaaattcgg taacaattat
     1201 agcgaaagta gatgacgtag aagaagtgac cagcgtgctt attttcgtcc gcgtgaaaga
     1261 tttaacggaa gaagattaca ccccatggac caatggcgat gtgatgttga accgcgggca
     1321 aggtgaattt acaaccgtgc tttatggtag caaaatcaaa ggtcacgatc actacttgcg
     1381 ctcatgggta tactttcaat tagtggccac aaatattgaa ggtgaagaga ttgggcgtac
     1441 caagatttac gataaagcat ttgatatgta tccatgcccg tgccttacgc cacttactgg
     1501 ctgcccaatc gccacaccaa aaccaccatc tccaactcct aaaaaataaa ccatgagaaa
     1561 aatattttta cttacgcttc ttcttatttt ctcccttgct tgttcattag ggacgttcgc
     1621 tccgcaacca actgcaaccc cagcgccaac gcagacggac accgcaacgc ctcttcctac
     1681 gccaacgcct gtaacgccaa ctctgacgtt tacctcaact ccaacactca tcagcaatac
     1741 agctattcca acaattgaaa ccacttcaac cgagctttca actccaactg ccacacaggc
     1801 attgtcgact ccttatacgg tgatgcctcc cgtaaatatt aaaggcttta tatccatcac
     1861 gatctcactg accgaggtgt ttaaggcaaa aggatgtgag ccttcagtgg ttcgtatcac
     1921 tgcacaggta gtaaacccaa atgaagtttc ttttgttctt ttattctcac gtttcaaaag
     1981 cttgaaagca gaacgtaccg gtaaatggac aactgttcag atgatcccat taggtgctgg
     2041 aacgtatatg ttcgaccttt cttctgcgat catgaaagat gatgcctatt tcgaaaacgc
     2101 atggatcgaa taccaagtag tctctacaaa ccaaaagggc acagaattgg gtcgcactga
     2161 tattttcaaa gagcgtctta agatggtaca gtgcatcttt actgccaccc ccacatctgc
     2221 ca
//
LOCUS       DAOQJE010000664         2220 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_37757_length_2220_cov_5.454503, whole genome shotgun sequence.
ACCESSION   DAOQJE010000664 DAOQJE010000000
VERSION     DAOQJE010000664.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2220)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2220)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2220
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37757_length_2220_cov_5.454503"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..275
                     /locus_tag="PLT08_14940"
     CDS             <1..275
                     /locus_tag="PLT08_14940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE05820.1"
                     /translation="LLKKHLPEEVANGLISDAQYKTAVSFFQFGARMSALSGGAYRAT
                     DHFYQYLGELAHKKEQLAKFGDERGNAAIIEKLRANIASLAAQAKT"
     gene            362..433
                     /locus_tag="PLT08_14945"
     tRNA            362..433
                     /locus_tag="PLT08_14945"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:395..397,aa:Arg,seq:ccg)
     gene            627..1637
                     /locus_tag="PLT08_14950"
     CDS             627..1637
                     /locus_tag="PLT08_14950"
                     /inference="COORDINATES: protein motif:HMM:NF019844.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HNE05821.1"
                     /translation="MTVPEFVTATLPVPATMAFTATPITEMPSNETIAPTTTPIAGTT
                     TSEVNVRADTSTVSSSLGTVPAFNNVLVTGKDPSGIWYQILFNNGAGWVRADFIQLVD
                     TTVEIPVVVPKTGSEAVSRGVVLRGVNVRSGPGQNFDSLGLLNQNDVIPVYGKDNSGE
                     WIKIGFSPAVDGTGWVAAEFLQLDNIASIPIVDEVIEPSPTAVSNDRPTLQPNSPIQN
                     SSQNDADTAQSPVATFVLSQAGTRSIQFQGELSTTNGDNEDWIGFSSEIKEVVIQVSC
                     DAASITVELVQEGATNNDQVECGKPKIIKVTSWQEYLIKVTSKPVSESLVEYKLNIKI
                     IK"
     gene            1662..>2220
                     /locus_tag="PLT08_14955"
     CDS             1662..>2220
                     /locus_tag="PLT08_14955"
                     /inference="COORDINATES: protein motif:HMM:NF016648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FecR family protein"
                     /protein_id="HNE05822.1"
                     /translation="MKRYLVVLSILVIIGVILTGCQTKEETSPLSAGLSELTGLVEMK
                     QAGQDAFTQAAADAVLEVNGQIQTGDDGRVRLDLSSGTIIRVAPSSFFTLTSNDEVEG
                     GLLTKIKLEAGKIFIVLNGGQTDVETPSGVASVRGSYLKVEVDPVTKDIHITCLEGTC
                     SATNPNGEQIIFTNGQSVILFHQESD"
BASE COUNT          643 a          494 c          497 g          586 t
ORIGIN      
        1 gtttattgaa gaagcatcta cctgaagaag tagcaaatgg attaatttct gatgcacagt
       61 ataaaacagc cgtctctttc ttccagtttg gggcacggat gtccgctctg agtggcggcg
      121 cttatcgcgc taccgatcac ttctatcaat accttggcga attggctcac aaaaaagaac
      181 aactggcaaa atttggtgac gaacgtggaa acgccgcaat catagaaaag ctacgggcga
      241 acattgccag ccttgccgcg caggcgaaga cctaagatat ttcgcttcat ctctaaatgt
      301 agggctggcg gctgctccgt cagccctttt ctacgcctat ggtaaaattc gcttcgctca
      361 agcccccata gctcagtgga cagagcgacg gcctccggag ccgtagagtg cagttcgagt
      421 ctgcatgggg gtgcctcaaa acatccgcaa agaagcggat gtttttctat ttatcccccc
      481 aaatgggtga ttaagaaccc ctttgtaaca attactacca cccatttttt agatacaatt
      541 atcctgtgaa cctaaaacat tcaagtgccc tttttatcat cattcttttg tgcacagggt
      601 gcgacgcatc tttatcaaca gcaactgtga ctgtacctga gtttgtaact gcgacattgc
      661 ccgttccagc cacaatggcg tttactgcta ctccaataac agaaatgccg tcaaatgaaa
      721 caattgcgcc tacaactaca cccattgctg gaacgaccac gtcagaggtc aatgttcgtg
      781 cagatacatc tacagttagt tcgtctttag gcacggtccc tgcctttaat aatgttctgg
      841 taacaggcaa agacccaagt gggatttggt atcaaatctt gttcaacaat ggtgccgggt
      901 gggttcgagc cgattttatt cagttagtag acacaactgt tgaaatccct gttgtcgtac
      961 caaaaacagg aagtgaggct gtaagtagag gcgtagtttt gagaggcgtt aacgtacgca
     1021 gcggaccagg tcaaaacttt gattcgttag ggttattaaa tcaaaatgat gttataccgg
     1081 tttatggaaa agataattct ggcgaatgga taaagattgg gttttcacct gcagtggatg
     1141 gcaccggctg ggttgctgca gagtttttac agcttgataa tatagcttcc atcccaattg
     1201 tggatgaagt cattgagccg tcacctacag ctgtttcaaa cgaccgtcct acccttcagc
     1261 ccaattcacc catacagaac tcgtcacaga atgatgccga tacagcgcaa tcgcctgtag
     1321 ccacctttgt actttcgcag gcgggaacac ggtctattca atttcagggc gaactatcta
     1381 ctaccaatgg agataatgaa gactggatag gcttttcgtc cgaaataaaa gaagttgtaa
     1441 ttcaagtttc atgcgatgcc gctagcatta cagtagaatt agttcaagaa ggtgcaacta
     1501 ataatgacca agtagaatgt ggaaagccta aaatcattaa agtcacttca tggcaggaat
     1561 atctcattaa agttacatcg aagcctgtca gtgaaagcct agtcgaatac aaattgaaca
     1621 taaaaattat caaataaagc accaaacgaa cggagagaga aatgaaaaga tatcttgtgg
     1681 ttttatcaat actcgtcatc ataggggtga ttcttacggg atgccagacc aaagaggaaa
     1741 catcccctct ttctgctggg cttagtgaat taacgggact tgtggaaatg aagcaagctg
     1801 ggcaagatgc ctttactcaa gccgcagcgg atgccgtact tgaagtgaat gggcagattc
     1861 aaactggaga cgatgggcgc gtacgccttg atctttcttc tggaacgatt attcgtgtgg
     1921 cgccatcttc atttttcacc ctaacctcaa atgacgaagt agaaggcggt ctgttaacca
     1981 aaataaagct cgaagcaggg aaaattttca tcgtcctcaa cggtggtcaa actgacgtgg
     2041 aaacaccctc tggtgtagct tcagtgcgcg gctcatattt aaaagtagaa gtggaccctg
     2101 tcaccaaaga tattcatatt acttgtctcg aaggaacttg ttctgctact aacccaaatg
     2161 gagaacagat catttttacc aatgggcaat ctgtaattct tttccatcaa gaatccgatg
//
LOCUS       DAOQJE010000665         2217 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_37866_length_2217_cov_6.047179, whole genome shotgun sequence.
ACCESSION   DAOQJE010000665 DAOQJE010000000
VERSION     DAOQJE010000665.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2217)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2217)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2217
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37866_length_2217_cov_6.047179"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..297)
                     /locus_tag="PLT08_14960"
     CDS             complement(<1..297)
                     /locus_tag="PLT08_14960"
                     /inference="COORDINATES: protein motif:HMM:NF013843.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HNE05823.1"
                     /translation="MNKLFVIVGASGVGKTTLMNALLTKYDCAIAYEQHDERPFQKLF
                     KEDARYALANQVDYLLMRAEQERELRSGNKPALMDGGFDLDFYGFTRLFHTHGWL"
     gene            complement(346..>2217)
                     /locus_tag="PLT08_14965"
     CDS             complement(346..>2217)
                     /locus_tag="PLT08_14965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE05824.1"
                     /translation="AYMPFDVFNVRTLPYHITSEGLPDPAYIRSDIKAIARLLKDTWA
                     SDRELVHSPIYNLLTGLKEPSRKSLQTPLATGFWREYEEWKDRVTIAQRNKHIGDILL
                     LTEEIRNPLIREDAVAEVGGALARLGRNELALKQYREGIDVNGENLEFRREEAFHLNR
                     VGRVNEAIVKLESILNDYPEDNKSISYLGRIYKEMWTNSWIKVKDKNKRLRLAFESYH
                     WLIQSIKIYMKGFQVDLRDYYPGINAYTLSKIAIHLADKFDNKKTPDPDITRIRHRLH
                     GLQETLEFCLENRLDLDQEKADYWSLVSLAELRLFTSNDIAEVLRSYRKAVTAVRRDL
                     FSLRSSLEQLETIHSLGIRTEFVSACIKMFKEEIKNASTGDAGNVIKVDNLVRPGTKN
                     KGARALVFSGYMVDFAGKDKKTFPPEKESEIRQEIKKRVDKFKPGPNDRAFLAGLSAG
                     SEIIFAEICAEAGIKVKVFLPHSDSSYIRRFVTPAGESWVDRFYKIRNHSLVDEIYQI
                     EHLGQPKDDDNVYERNSRWAMYSAMGRVGIDNMTLIAVASEFIGDTKDRDIQITRYTI
                     ELMRNLGGRVEEFINPTKYIYSVIDSALERLIAQGGSSGEKKITPTKLRKRKSERKV"
BASE COUNT          547 a          489 c          511 g          670 t
ORIGIN      
        1 gagccagcca tgagtatgaa agaggcgtgt aaatccataa aaatcaaggt caaacccgcc
       61 atccattaaa gcgggtttat tgccagagcg taactcgcgt tcttgttcgg cgcgcatcaa
      121 tagataatca acttgattcg ccagcgcata gcgtgcatct tctttgaaaa gtttttggaa
      181 tggacgctcg tcatgttgct catatgcaat agcgcaatca tactttgtaa gcaaggcatt
      241 cataagcgtg gttttgccta ctccgctggc acctacaatg acaaagagtt tattcatagc
      301 gtttaggatg aatagacccg ctgcaaaagc gggtctatgg ttttgctaaa cctttctttc
      361 agattttctt ttcctcagtt tggtaggtgt aattttcttt tcgcctgaag aaccaccttg
      421 tgcaatgagg cgttcaagag cgctatcgat tacgctgtaa atatacttgg tggggttgat
      481 aaattcttca accctgccac ccagattgcg catcaattca atcgtgtaac gagtgatttg
      541 aatatcgcgg tctttggtgt cgccaatgaa ttcgcttgct acagctatta gtgtcatgtt
      601 atcaatgcca acgcgcccca tggctgaata cattgcccaa cggctgtttc gctcatagac
      661 attatcgtca tctttgggct gaccgaggtg ctcgatttgg tagatctcat ctactaatga
      721 gtggttgcga attttgtaga agcgatccac ccaactttcg ccagcgggtg ttacgaaacg
      781 gcgaatataa ctggaatcag agtggggtaa gaacaccttg acctttatgc cggcttcggc
      841 acaaatctct gcgaagatga tttcactacc cgcagataac cccgctaaaa atgcgcggtc
      901 attgggtccg ggtttgaact tgtcaacacg tttcttgatt tcctgccgga tttcactttc
      961 tttttcgggc gggaatgtct ttttatcttt tcccgcaaag tcgaccatgt agccactaaa
     1021 tacgagtgct cgagcccctt tatttttcgt gcctgggcgt accaagttat ctaccttgat
     1081 gacattgcct gcatcgcctg tgctggcatt tttgatttct tccttgaaca ttttgataca
     1141 agctgaaaca aattcagtgc gaatgccgag agaatgaatc gtttccagtt gttcaaggga
     1201 tgaacgtaga gaaaatagat cgcgtcgaac ggctgtgact gccttgcgat aggagcgcaa
     1261 aacttcagca atgtcattgg aagtgaagag ccttaattct gccaaagata caagagacca
     1321 gtaatcagct ttttcttgat caagatcgag ccgattttcc agacaaaatt ctagcgtttc
     1381 ctgtaaccca tgcagtctgt ggcgaatgcg agtgatgtct gggtcgggcg tctttttgtt
     1441 gtcaaactta tctgcgaggt gaatggcaat tttggaaagg gtgtatgcat tgatcccagg
     1501 gtagtaatcc cgcaggtcaa cttgaaatcc cttcatataa atcttgatgg attgtatgag
     1561 ccagtgatag ctttcaaatg ccaagcgaag gcgcttattc ttgtctttaa ctttgatcca
     1621 agagttggtc cacatctctt tgtagatgcg tccgagatat gaaattgatt tgttgtcttc
     1681 tggataatca ttcaaaatac tttcaagttt tacgatggct tcattcaccc ttcctacacg
     1741 gttaaggtga aaagcttctt cccggcgaaa ttcaagattt tccccgttga cgtcgattcc
     1801 ttcgcggtat tgttttagag ctaactcgtt acgtcccaac cgggcaagtg cgccgcctac
     1861 ttcagcaact gcatcttcgc gaatgagcgg gtttctgatt tcttcagtta agagcaaaat
     1921 atcaccaatg tgtttattgc gttgagcaat ggtgactctg tctttccatt cctcatattc
     1981 gcgccaaaag ccagttgcta agggggtttg caaacttttg cggcttggtt ccttaagccc
     2041 tgtgagcagg ttgtagattg ggctatgtac tagttctcgg tctgaagccc aggtatcttt
     2101 gagaaggcgc gcaattgcct taatgtcact gcgaatatat gccggatctg gcagaccttc
     2161 tgatgtgatg tgatagggca aagtgcgtac gttgaacaca tcaaatggca tgtaagc
//
LOCUS       DAOQJE010000666         2217 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_37877_length_2217_cov_3.831175, whole genome shotgun sequence.
ACCESSION   DAOQJE010000666 DAOQJE010000000
VERSION     DAOQJE010000666.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2217)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2217)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2217
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37877_length_2217_cov_3.831175"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1220
                     /locus_tag="PLT08_14970"
     CDS             <1..1220
                     /locus_tag="PLT08_14970"
                     /inference="COORDINATES: protein motif:HMM:NF013714.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HNE05825.1"
                     /translation="AGDVSRLVSLAREHGFELAVRSGGHSNAGYCTSDGGIVLDLSAM
                     KKLEIDHENQTAWVETGTTAGEYTAAVGTHGFVTGFGDTGSVGLGGITLGGGVGYLVR
                     KYGLTIDHLLAAEVVTADGQLLHVDENSHADLFWAIRGGGGNFGVATRFKFKLHKLDQ
                     GYGGMLVLPATADAIASFLAEADSAPDELSTILNIMPAPPMPFLPEEVHGKLILMAMM
                     FYAGDAAMGEQVISKFRAIATPYADMLRPMAYTEMFPSEEGGYHPVAAGRTMFLDHVD
                     SSVAQFILDTLAKSTAMMAVTQLRVLGGAYARVPADATAYAHRGLKIMANLASLYGNP
                     EEKEHHEAWVAKYEKKLRQSDKGAYVNFLGDVDQKQVRAGYPGDAWKRLGKVKKKYDP
                     TNLFRLNQNIPPK"
     gene            1229..1810
                     /locus_tag="PLT08_14975"
     CDS             1229..1810
                     /locus_tag="PLT08_14975"
                     /inference="COORDINATES: protein motif:HMM:NF013989.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HNE05826.1"
                     /translation="MSKVLLDMSMSLDGFIAGANVGKEYPMGVNGEDLHTWMFSTPKD
                     KVDAEVEREMFARTGAVILGNRTFEIGVDLWGDVPFPAPSFVLTHKKREPLIKKSGTF
                     TFVTDGIESALQQAQAVAGDKDIRLMGADVAQQFLSAGLVDEIQINHIPILLGAGVRL
                     FEHLGVKNSQLKKDRVLDSPHATHIHYRVLKGK"
     gene            1820..2194
                     /locus_tag="PLT08_14980"
     CDS             1820..2194
                     /locus_tag="PLT08_14980"
                     /inference="COORDINATES: protein motif:HMM:NF019013.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HNE05827.1"
                     /translation="MTDTNTMTAVTFLKLASSGKVSEAYAKYIGLGFTHHNPFFEGSA
                     EALQAGMEENAKQSPNKVFTILRTISEGDFVVTHSHVQQSPEQRGAVVVHIFRFENGK
                     IVELWDVGQPIPETSANQNGMF"
BASE COUNT          561 a          519 c          587 g          550 t
ORIGIN      
        1 atgcgggtga tgtatcgcgt ctcgtttcgt tggcgcgtga acatggcttt gaactcgccg
       61 ttcgcagcgg tggtcacagc aatgcgggtt actgtactag cgatggcggt attgtgctcg
      121 acctctctgc aatgaaaaaa ttggagatcg atcacgagaa tcaaaccgcc tgggttgaaa
      181 ctggcacgac tgctggcgaa tacacagccg ctgttggcac acatggattt gtcaccggct
      241 ttggcgatac gggttcggtc ggtctcggcg gcattacgct tggcggcggc gttggctatc
      301 tcgtccgcaa atatgggttg accatcgatc atctgctcgc cgcggaagtg gtcacagccg
      361 atggacaact cttgcacgtg gatgaaaatt ctcatgccga tctcttctgg gcgatccgcg
      421 gcggaggcgg caacttcggc gtggcgacgc gcttcaagtt caagcttcat aagttggatc
      481 aaggctacgg cgggatgtta gtcctgccag ccacagcaga tgccatcgct tctttcttag
      541 ccgaagctga ttctgctccc gatgaattgt ccaccatcct gaacattatg cctgcgcccc
      601 ctatgccttt ccttccagaa gaggtgcatg gcaaattgat cttgatggcg atgatgttct
      661 atgccggtga tgcagcaatg ggggagcagg tcatctccaa gttccgagcc attgccactc
      721 cttacgcgga catgctgcgt ccgatggcgt atacagaaat gttcccatcc gaagaaggcg
      781 gctatcaccc cgttgctgcg gggcgcacta tgttccttga ccatgtggat agttctgttg
      841 ctcagttcat tcttgatacc ttagccaagt ctacggcgat gatggcagtg actcaactgc
      901 gcgtgttggg cggagcctat gcacgcgtcc ctgccgatgc gaccgcatat gcccatcgcg
      961 ggttaaagat catggcaaac cttgcctcct tgtatggcaa cccggaagaa aaagaacatc
     1021 acgaagcctg ggtcgctaaa tacgaaaaga aactgcgtca aagcgacaaa ggcgcgtacg
     1081 tcaacttcct tggtgatgtg gaccaaaaac aagtccgcgc ggggtacccg ggtgacgcat
     1141 ggaaacgact tggcaaggtc aagaaaaaat acgatccgac gaatctattc cgcttgaatc
     1201 aaaatattcc cccaaaatag gattcgatat gagtaaagtt cttttagata tgtcgatgtc
     1261 gctggatggt tttatcgcag gtgcaaatgt gggcaaagaa tatcccatgg gtgtgaatgg
     1321 tgaggacctt catacctgga tgttcagcac acccaaggat aaagtggacg ctgaggtgga
     1381 acgcgagatg ttcgcgagaa cgggagcggt catattaggg aaccgcacct tcgaaatcgg
     1441 tgtggatttg tggggcgatg ttccttttcc tgcgccgtct tttgtgctta cccacaagaa
     1501 gcgcgaaccg ttaatcaaga aaagtggaac ctttacattc gtaaccgacg gcatcgaaag
     1561 cgcgcttcaa caagctcaag ccgttgcagg tgataaagac attcgtttaa tgggcgctga
     1621 tgttgctcaa caatttctca gtgcgggtct ggtggatgaa atacaaataa atcatatacc
     1681 gatccttctt ggtgctggtg tacgattatt tgaacatttg ggtgtaaaaa atagtcagct
     1741 caaaaaagac cgggtacttg actctcccca tgcaacccat attcactatc gtgttttaaa
     1801 gggtaagtaa ggatttatca tgactgatac aaatacgatg acagctgtaa ctttcctcaa
     1861 acttgcttca agcggaaaag taagtgaagc ctatgccaaa tatattggtc taggcttcac
     1921 tcaccataat cccttctttg aaggttcagc ggaagcgttg caagcaggta tggaagaaaa
     1981 tgcgaaacaa agcccaaaca aggtcttcac cattttgcgg acgatcagcg aaggtgattt
     2041 tgttgtcaca cattctcatg tccagcaaag ccctgagcag cgtggtgcgg ttgtcgttca
     2101 catcttccgc tttgagaatg gaaagatcgt agaattatgg gatgtagggc aacctatccc
     2161 tgaaacgtct gcgaatcaga atggaatgtt ttagaaaaag gagagtttgt aatggcg
//
LOCUS       DAOQJE010000667         2217 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_37878_length_2217_cov_3.745606, whole genome shotgun sequence.
ACCESSION   DAOQJE010000667 DAOQJE010000000
VERSION     DAOQJE010000667.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2217)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2217)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2217
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37878_length_2217_cov_3.745606"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..729
                     /locus_tag="PLT08_14985"
     CDS             <1..729
                     /locus_tag="PLT08_14985"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013704.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HNE05828.1"
                     /translation="DEGSICWQVGNYVDNGRIVPLDIVLYPIFESLGLQLRNRIVWHF
                     GHGLHASKRFSGRYEVIMWFTKSNNYTFNLDPIRVPQKYPQKKHFKGPKKGELSGNPL
                     GKNPSDIWDIPNVKSNHIEKTIHPCQFPIELIERLVLSMTNENDWVFDPFMGVGSAAI
                     ASLMHNRKVIGSEIMPEYIAIAKERINKAEKGELQMRPMDRPVYDPSAPVKIAPPKTI
                     KLKNKVIQKQLFEKKGKYKARKSK"
     gene            726..1274
                     /locus_tag="PLT08_14990"
     CDS             726..1274
                     /locus_tag="PLT08_14990"
                     /inference="COORDINATES: protein motif:HMM:NF020757.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009036 - type II site-specific
                     deoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BglII/BstYI family type II restriction
                     endonuclease"
                     /protein_id="HNE05829.1"
                     /translation="MKIVYEYSHLGGSEILHQRYPQHEKDIYKVVSLVKGNRSKISKE
                     KTKIGKALYSPIDMNSQFEKEFNNRGFHEIRDTYTISLPNYELEINGAFKQVDFVKDK
                     VLVEVQFGKYAFMFYDMAKFQYFFNENKADVGVEIVPCHSLHREMSSGVSYGEQLIYD
                     IERLKRHFPAVPVKVILVDIDN"
     gene            1291..1620
                     /locus_tag="PLT08_14995"
     CDS             1291..1620
                     /locus_tag="PLT08_14995"
                     /inference="COORDINATES: protein motif:HMM:NF015758.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphohydrolase
                     family protein"
                     /protein_id="HNE05830.1"
                     /translation="MNFNDYQQKSRATAQYPAIGHPVIYPALGLVNEAGEVAGKIKKI
                     FRDKQGNIGEAEREALKAELGDVLWYIAQVATELDLPLDEIAEANLAKLLDRQARGKI
                     QGDGDNR"
     gene            1621..1953
                     /locus_tag="PLT08_15000"
     CDS             1621..1953
                     /locus_tag="PLT08_15000"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux SMR transporter"
                     /protein_id="HNE05831.1"
                     /translation="MKTFIILFFAILSEVIATSSLKFSEGFTKPIPSVIVAIGYGLSF
                     YLLSIALKSMPIGVAYAIWSGIGLILTVIAGIVLWKETLDWARVLGIILILAGIVLIN
                     VFSKATVH"
     gene            complement(1994..>2217)
                     /locus_tag="PLT08_15005"
     CDS             complement(1994..>2217)
                     /locus_tag="PLT08_15005"
                     /inference="COORDINATES: protein motif:HMM:NF012790.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HNE05832.1"
                     /translation="ETQTNGVMKSPALLREMFNEYQNKKLIFSCGTGVTACILALAAD
                     QAGVKNLSVYDGSWAEWGAVDSGLPVVKN"
BASE COUNT          727 a          440 c          443 g          607 t
ORIGIN      
        1 gacgaaggaa gtatttgttg gcaagttggt aattatgttg ataatggaag aattgttcct
       61 ttagacatag tgctttaccc tatttttgaa tctttaggtt tgcagttgcg taatcggata
      121 gtttggcatt ttgggcatgg gcttcacgca tcaaaaagat tttcaggtag atacgaagtc
      181 ataatgtggt ttacaaaatc aaacaactac acttttaatt tagatccaat tagagttcct
      241 caaaaatatc cacaaaaaaa gcattttaag gggcccaaaa aaggtgaatt atcaggaaat
      301 cctttaggaa aaaaccctag tgatatttgg gatatcccaa atgtaaagtc aaaccatata
      361 gaaaagacta ttcacccatg tcagtttcca attgaattaa ttgaaagact tgttttatca
      421 atgacaaatg aaaatgactg ggtcttcgat ccatttatgg gggttggaag tgctgctatt
      481 gcatctttga tgcataacag aaaagttatt ggctctgaaa taatgccaga atacatcgca
      541 atagccaaag aacgtattaa caaggctgaa aaaggtgaat tacagatgag gcctatggat
      601 cggccggttt acgatccttc tgctcctgta aaaattgcgc cgccaaaaac tataaaatta
      661 aaaaataaag ttattcaaaa acaactattt gagaaaaagg gaaaatataa agcgaggaaa
      721 tcaaaatgaa aatagtttat gaatattctc atcttggcgg atcagagata ctacatcaaa
      781 gatatccaca acacgaaaaa gatatttaca aagtagtgtc cttagttaaa ggtaatagat
      841 caaaaataag caaagagaaa acaaaaatag gtaaagctct ttactcacct atcgacatga
      901 atagtcaatt tgaaaaagaa ttcaacaaca ggggtttcca tgaaataaga gacacttaca
      961 ctatatcact tccaaattat gaacttgaaa ttaacggggc atttaaacag gtagattttg
     1021 taaaagataa agttttagta gaagttcaat ttggtaaata tgctttcatg ttttatgaca
     1081 tggcaaagtt tcaatacttt ttcaatgaaa ataaagcaga tgtaggtgta gaaattgttc
     1141 catgccatag tttgcataga gaaatgtctt ctggcgtctc ctatggagaa caacttatat
     1201 atgacattga acgtctaaag cgccactttc ctgccgtacc tgtaaaagtt attttggtag
     1261 atattgacaa ctaaaacaag ggacaagttc atgaacttca acgactatca acagaaatca
     1321 cgtgccacgg cgcaatatcc tgccattgga caccctgtca tttacccggc tcttggcttg
     1381 gtcaacgaag cgggcgaagt agcaggcaag atcaagaaaa tctttcgaga taaacagggc
     1441 aacattggcg aagccgaacg agaagcgctc aaagccgagc tgggtgatgt gctttggtac
     1501 attgcacaag tcgccaccga actcgatctt ccgctcgatg aaattgccga agccaacctc
     1561 gccaagctat tagaccgtca ggcacgcggc aagatccaag gcgacggcga taatcgctaa
     1621 atgaaaacct tcatcatctt attcttcgcc attctgagtg aagtgatcgc cacatcgtcg
     1681 ctcaagtttt cagaaggctt caccaaaccc attcccagtg tcattgtagc gatcgggtac
     1741 ggactgtcct tttatctttt gtccattgcg ttaaagtcca tgccgattgg ggttgcctat
     1801 gcgatttggt ctggtatagg actaattctc accgtcattg ctggcatcgt tctctggaaa
     1861 gaaaccttag actgggcacg agtacttgga atcattttga tcctggcagg cattgtgttg
     1921 atcaatgtct tttcaaaagc gactgttcat taaacaaaaa agcggatgaa acatccgcta
     1981 cagaacgatt ttttcaattc ttcacaaccg gcaagcctga atccacggcg ccccactctg
     2041 cccacgagcc gtcgtaaacc gataaatttt tcaccccagc ttggtccgcc gccaacgcca
     2101 aaatacaagc cgtgactccc gtgccgcaac taaagatcaa ttttttattt tgatattcgt
     2161 tgaacatctc acgcaaaagc gcaggggatt tcattacgcc gttggtttga gtctcac
//
LOCUS       DAOQJE010000668         2212 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_38037_length_2212_cov_4.881317, whole genome shotgun sequence.
ACCESSION   DAOQJE010000668 DAOQJE010000000
VERSION     DAOQJE010000668.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2212)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2212)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2212
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38037_length_2212_cov_4.881317"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            261..>2212
                     /locus_tag="PLT08_15010"
     CDS             261..>2212
                     /locus_tag="PLT08_15010"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HNE05833.1"
                     /translation="MMQSSSFSRKLSEENNSLVRTLRRRADLHPERLAFRFLQDDESE
                     NITINFGELDRRARAIGAWLESFNAKGERALILYPPGLDYIAAFMGCLYAGVIAVPAY
                     PPRLNRPVPRIQSIVDDSHATFALTTTSIHSNIEKRFEHAPDLKALHWLNTEQVPAGL
                     EADWRDPNVSPDALAFLQYTSGSTSQPKGVMLSHGNLMHNLEAISRGFHIDETVMGIF
                     WLPSYHDMGLIGGILEPMYLGGATTLMSPPSFLQRPVRWLEAITRYRGNTCGAPNFAY
                     DLCVDKVTSEQKETLDLSSWSLVFCGAEPIRPETLERFSRAFAGCGFRKTSFYPCYGL
                     AESSLIVSGADGPSELRTFTVDRKSLESDLVVPAQASEEGSVTMVNCGPSIFDQKIII
                     VNPNTLLQCEPNQVGEIWVSGPSVAQGYWGLEEETRRTFQAHVADTGEGPFMRTGDLG
                     FLQNGELFVTGRLKDLIIIHGSNHYPQDIELTVETCHPALQPAGGAAFSITEGDKEQL
                     VVVQELNRQNRQADVNEVAAAIRQAVADKHDLQIFAIVLIKPMSIPKTSSGKIQRRAC
                     KTAFLNGELEVVGEWRAKKSAVPSPQKEKTESLPAKPAKPGINSNALQTWLVTRIASM
                     LEMDATSIDPRQPFTYYGLGSVQAVSL"
BASE COUNT          562 a          501 c          532 g          617 t
ORIGIN      
        1 taagacaaga ttataccttt ttattaaaat gcatgtcaac ctaccaatgg gggatacgta
       61 aaatcactga aaatattgtg tttgttttca cttgctttgc taaaaaattg gtcttttacg
      121 atggcttgaa aattttcgtt tagcttgaat gttaataatt tcgccctact gatgggctat
      181 atagtttggt gctataaatg tgttacgctt aaattttatg atataaattc aaaatcttgt
      241 tcttttaatg gtgaaaatac atgatgcaat cttcttcttt ttcccgaaag ctatccgaag
      301 aaaacaattc actggttcgt actctgcgga ggcgggcaga cttgcatcct gaaaggctgg
      361 cgtttagatt tttacaggat gatgaatcag aaaatatcac catcaacttt ggggaacttg
      421 atcgtcgggc acgtgccatt ggggcatggt tagaaagctt caacgcaaaa ggggagcgtg
      481 cacttattct ctatccacct ggattggatt acatcgctgc ttttatgggc tgcttatatg
      541 ctggtgtaat cgctgtccct gcatacccgc ctcgccttaa ccgccctgta ccccgtatcc
      601 aatctatcgt tgacgattca catgcgacct ttgcgctgac tacaacgtcg atccattcga
      661 acatcgaaaa acgatttgaa catgctccag acttgaaagc gctacattgg ttaaacaccg
      721 agcaggtacc ggcggggttg gaagcggatt ggcgcgatcc taatgtctcg ccagatgcgc
      781 tggcattcct tcaatataca tccggctcta ctagtcagcc aaaaggagtc atgcttagtc
      841 atggcaacct gatgcataat cttgaagcca tcagccgcgg gtttcacatc gacgagacgg
      901 tgatgggaat tttctggctg ccaagttatc acgacatggg gctgatcggc ggcatccttg
      961 agccgatgta tttaggtggt gcaacgacct tgatgtcgcc gccatccttc ttacagcgac
     1021 cagtacgttg gctcgaagcc atcacccgtt atcgtggcaa tacctgcggt gcgccaaact
     1081 ttgcctatga tctgtgtgtt gataaggtta catcggaaca gaaagaaact ctggatttga
     1141 gttcatggtc tcttgtgttt tgcggtgcag aaccgattcg tcctgaaacg ttagaacgct
     1201 tctcacgtgc ctttgccggg tgcggcttcc gaaagacttc tttctaccct tgttatggat
     1261 tggcggaaag ttccctgatc gtctccggtg cggatggtcc atctgaactg cggacattta
     1321 cagtggatcg gaagtcactt gaaagcgatc ttgtcgttcc tgcccaagca agtgaagaag
     1381 gttctgtaac gatggtgaac tgtggtcctt cgatttttga ccagaagatt atcattgtga
     1441 atcccaacac gttgctacaa tgtgaaccaa atcaagtcgg cgaaatttgg gtttctggtc
     1501 ccagtgtggc gcagggatat tggggtctgg aagaggaaac ccgccgaaca tttcaagctc
     1561 atgtagcgga tacgggcgaa ggtcccttta tgcgcacagg tgaccttggc tttctgcaaa
     1621 acggagaact ctttgtaaca ggtcgcttga aggacttgat cattattcat ggcagcaatc
     1681 attatcccca ggatattgaa ctgaccgttg agacatgcca ccctgcttta caacccgctg
     1741 gtggagcagc attttctatc acggagggtg ataaggaaca gttggttgtt gttcaagaat
     1801 taaacaggca aaatcgacaa gcagatgtca atgaagttgc cgcggctatt cgtcaagcag
     1861 tggcagataa gcatgacctg cagattttcg ccatcgttct gatcaagccg atgagcattc
     1921 caaaaacatc cagcggaaag attcaacgcc gcgcttgtaa aacagctttt ctaaatggag
     1981 agttggaagt tgtgggtgaa tggcgcgcga agaagtctgc cgttccttct cctcaaaagg
     2041 aaaaaactga atctcttcct gctaaaccag cgaagccggg gattaattca aatgctcttc
     2101 aaacttggct ggtgactcgc atcgcctcaa tgctggaaat ggatgcgaca tcgattgatc
     2161 cccgtcagcc gtttacctat tatggactcg gttctgttca agccgtcagc ct
//
LOCUS       DAOQJE010000669         2209 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_38106_length_2209_cov_7.973073, whole genome shotgun sequence.
ACCESSION   DAOQJE010000669 DAOQJE010000000
VERSION     DAOQJE010000669.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2209)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2209)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2209
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38106_length_2209_cov_7.973073"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(192..683)
                     /locus_tag="PLT08_15015"
     CDS             complement(192..683)
                     /locus_tag="PLT08_15015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE05834.1"
                     /translation="MKTLGGVIMKDYWAILNYEIQMYFGARHLQSFPINGASQSLTTL
                     QASAMTEVKALHVRILLDIFLERKNSDDINIDDILPDWREKNQTVVHNLEIAYNKKLE
                     IGKTPKWYLNKYLAHPDKRRGDHFDWAPVIKRMDTPLKEVFKTLPTEKLHALVFFQKY
                     LYA"
     gene            complement(789..2078)
                     /locus_tag="PLT08_15020"
     CDS             complement(789..2078)
                     /locus_tag="PLT08_15020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE05835.1"
                     /translation="MNQKVIIFFAIILLAACSSSPSRVSTESPSVEPSQTSIPSPLPL
                     PSDTPEPSPTVTPIPIGGGQLLVAFYASGSCGACIIIGDFFTGEILFEIPLSTDFQTG
                     NIFWSPDGESILYSDITTARMNVLLFDLETGQSKKLGDHPRKGGSPEFVNSMKYVEWS
                     YDSEYVMYDVYYEDSNLQKSYFASKSGVPGFYESGFTDWFPDSRTMFSIYGGKDSYNV
                     ETNTFVPTSTDVLSKFNVVYILKDFILLEREKTKISVIPFPENWNDALAWQYDALGSQ
                     VVVLAQLSEEIKNGQVHVYVADKIDDNKVSVIGSVLVSNQMSYFVKIIDLKNLPVEIK
                     PDDLLQQIGDTPLVVSPDGDYYLKGYCTFMETCLNYNPNWKNEIVRGWGFQVVGFNGV
                     EQVLPVDLSQFKGVTKTTANVINTSDGLLDGIAFYWK"
BASE COUNT          658 a          517 c          397 g          637 t
ORIGIN      
        1 atatgtatct gattccctgt gaattatgaa aactcaatca actgcccaac ggattgcgtt
       61 acccacgcag agacggcgaa gccgtccaac caaaaaaatg acaaggcgta gaaaatgctt
      121 gagattgccg cacaccttac ggcagtcccc agcgtcagat gcacgctttg ttaggcgttt
      181 tttgactcac cttatgcgta aagatatttt tgaaaaaata cgagtgcgtg taacttttct
      241 gttggtaaag ttttgaaaac ttcctttaga ggcgtatcca ttcgtttaat cactggagcc
      301 caatcaaagt gatctcctct ccttttatct gggtgggcaa gatatttgtt caaataccac
      361 ttaggcgtct tgccgatttc aagtttttta ttgtaagcaa tttccaaatt gtgaacaacg
      421 gtttgattct tctctcgcca atccgggagg atatcatcaa tgtttatgtc gtcactattt
      481 tttctttcaa ggaatatgtc aagcaaaatc cgaacatgga gggcttttac ttctgtcatt
      541 gcacttgcct gtaaggttgt caggctttgg ctagcaccat tgattggaaa actttgaagg
      601 tgtcgtgcac cgaaatacat ttgtatctca taattcaata tcgcccaata gtctttcata
      661 atcacacctc caagagtttt catggcacac ccatccaact aagttttata catctaactg
      721 tgtaacaccc tatatacatc cccaaacaac aactgcccct tgctggccca tttttatttt
      781 caaagccatc acttccaata aaaggcaatt ccatcaagca gcccatcgct tgtattaatg
      841 acattggcag tcgtcttcgt cacccctttg aactgggata aatctactgg aagcacctgt
      901 tcaacgccat taaacccaac gacctgaaac ccccaccctc tgacaatttc atttttccag
      961 tttgggttgt aattcaaaca cgtttccata aaagtgcaat aacctttcaa gtagtaatcc
     1021 ccatcgggcg agacgacaag cggcgtatcc cctatttgct gcaacaaatc atctggcttt
     1081 atttcaacag gcaagttttt caagtcgatt attttcacaa aatacgacat ctgatttgaa
     1141 accaagacag atccaataac agatacttta ttgtcatcaa ttttatcggc aacataaaca
     1201 tgtacctgtc catttttaat ttcttcagaa agctgagcca aaacaacgac ttgcgaacca
     1261 agcgcatcgt attgccaggc cagagcgtca ttccaatttt caggaaacgg aatgactgag
     1321 atctttgtct tctccctttc caacaggata aagtctttca aaatataaac aacgttgaat
     1381 ttacttaaca catctgtaga tgttggcaca aatgtgttcg tctccacatt gtacgaatct
     1441 tttccgccat aaattgagaa cattgtcctg ctatcgggaa accagtcagt aaatccgctt
     1501 tcgtaaaaac caggcacccc gcttttggag gcaaaataag acttttgaag attactgtct
     1561 tcataataca catcatacat aacatattcg ctgtcgtatg accactcaac atacttcatt
     1621 gaattaacga attctggcga tccacccttc cgcgggtgat cacccagctt tttagattgc
     1681 cccgtctcca aatcaaacaa aagaacattc attctggctg tagtgatatc ggaataaagg
     1741 atgctttccc catcgggaga ccagaaaata ttccccgttt gaaaatctgt tgataaaggg
     1801 atctcgaata aaatttctcc cgtaaaaaaa tctcctatta tgatacatgc accacaactg
     1861 ccagatgcat aaaatgcaac aagcaattgc ccgccaccga tcggaatggg agtgacggtt
     1921 ggggatggct caggtgtatc tgacggcaag ggtaatggag agggaatgga agtctgggaa
     1981 ggttcaacgg acggagattc agttgataca cgggaaggac ttgacgaaca agccgccaaa
     2041 aggattatag cgaaaaaaat gatgactttt tgattcatat ttttcccttt tgaatatctt
     2101 tttttactta tggtttttat tgcggaaggt gcaagcgcaa acaccttaca cgcatcaaat
     2161 tatacgcccc caaacaacaa ctatccctcc ccattccact cgatatcga
//
LOCUS       DAOQJE010000670         2206 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_38194_length_2206_cov_10.336123, whole genome shotgun
            sequence.
ACCESSION   DAOQJE010000670 DAOQJE010000000
VERSION     DAOQJE010000670.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2206)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2206)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2206
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38194_length_2206_cov_10.336123"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..396
                     /locus_tag="PLT08_15025"
     CDS             <1..396
                     /locus_tag="PLT08_15025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE05836.1"
                     /translation="SKDLDLINTSFSRRSKIKAVMERLGFHEQGRYFIHPETTFFIEF
                     PDGPLSVGEEPVKEISEFELSTGTLRVLSPTDCVKDRLCAFYFWNDLQGLEQATLVTK
                     SQKVDLKEIKRWSKVEGKVKEFKAFTEKL"
     gene            478..2139
                     /locus_tag="PLT08_15030"
     CDS             478..2139
                     /locus_tag="PLT08_15030"
                     /inference="COORDINATES: protein motif:HMM:NF025056.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003953 - NAD+ nucleosidase activity
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="toll/interleukin-1 receptor domain-containing
                     protein"
                     /protein_id="HNE05837.1"
                     /translation="MKPNIFMSYSRRETGFVDDLTYRLEKAGFNVWLDFRRLIPGTPW
                     AGQIDKGLRESDVVLLVVSKDSIASQFVELEWRHVLEYEHKRIILAIFEAVKLPPELK
                     KYEWVDFRGSYEAGLKELIGQLNTPMQEMQPAPETGFKVPLIVWVTVIFAFFLALSSL
                     YSFWTILIPLILLPLPLQVLKRNYNFSRIQSILVFQPVSLFLVVLLMFETGVFQNEAI
                     LGLSDFYYSLSPFFRFLYDAMQWMFFLSIFLPFFLLRTAGMQRWGKPEANMPKFANPY
                     KPNNPKPKPITFFIDHVEQDSVMAKDISKGLTRYGHIPAADLKSAEAVFALVSRFKTD
                     TEADPEKQTVFPVMVQTAQPSEKLSHVQWVDFRKGLRNIDAVAQLLPDPAKMLTALGV
                     RPTSGTQTVMPGIISAFVNFLLGMVFLDIGSLAAYVIELWAFDGGAIMLPGILYFGMY
                     IIAMFLIFGVIYLVNTSLTARRGWLASINGLIFSQALLFVLFYWQAAQVDNIDALYAQ
                     SGVTDPGGYFIMVPFLVYIAGGFLLSAAALFRFSDIRRWFPAKTT"
BASE COUNT          539 a          522 c          531 g          614 t
ORIGIN      
        1 tcaaaagacc tggacttgat caacacaagc ttttcacgac gcagcaagat caaagccgtg
       61 atggaaaggc ttggcttcca cgagcaggga cgttacttca tccatcccga aacaaccttc
      121 ttcatcgagt tccccgatgg accactatct gttggagaag aacctgtgaa agagatcagc
      181 gaattcgagc tttccacagg gacattgcga gtgctctccc ctacggactg tgtcaaagac
      241 aggttgtgtg cgttctactt ctggaatgac ttgcagggat tggagcaggc aaccttggtg
      301 acaaaaagcc aaaaagtgga cttgaaagaa atcaaacgtt ggtcgaaggt agagggaaaa
      361 gtaaaagaat ttaaagcatt tacagaaaag ctttgagctt ccccccacca cccgattgga
      421 ttgcagtgtg gtttaatatt tgaacgcagt acattcagcc tgccaaagga tcattaaatg
      481 aaaccaaata tctttatgag ttattcacgc cgcgaaactg gttttgtaga tgacctgacc
      541 tataggttgg aaaaggcagg gtttaatgtt tggctagatt tccgccgatt aattccaggc
      601 acaccttggg cagggcagat cgacaaggga ttaagggaat cagatgtggt cttattggtg
      661 gtttccaaag attccatcgc gtcgcagttc gtggaattgg aatggcgcca tgtattggag
      721 tatgagcaca agcgcatcat tctcgctatt ttcgaagcgg taaaacttcc gcctgaatta
      781 aaaaaatacg aatgggtgga ttttcgcggc agttacgagg cggggcttaa ggaactcatc
      841 ggtcaattaa ataccccaat gcaggaaatg caacctgcac ctgaaaccgg cttcaaagtg
      901 cccttgatcg tttgggtgac ggtgatcttt gctttttttc tcgccctttc ttcgctatat
      961 tcgttttgga cgatcttgat cccgctgatc ttgctcccgc ttcccttgca ggtactcaaa
     1021 cgcaactaca atttctcgcg gatccagagc atcctggtgt ttcagcctgt ttcactattt
     1081 ttggtcgtcc tgctaatgtt cgaaacgggc gtattccaaa atgaggcaat attggggttg
     1141 agcgattttt actattcctt atccccattt ttcaggttct tgtacgatgc gatgcaatgg
     1201 atgtttttcc tgtccatatt cctgccgttc ttcctgttgc gcactgcagg gatgcaacgc
     1261 tggggcaaac ccgaagccaa tatgccaaag tttgccaacc cgtataaacc aaacaatccc
     1321 aagcccaagc caattacttt ctttatcgat catgttgagc aggattctgt tatggcgaag
     1381 gatatctcaa agggattgac cagatatggg cacatccccg ctgcggatct taaatccgct
     1441 gaagcagtct tcgcgttggt ctcacgcttc aaaacggaca cggaagctga tccagaaaaa
     1501 cagaccgttt tccccgttat ggttcagacc gctcaacctt cagagaaact ctctcacgtg
     1561 cagtgggtcg attttcgtaa ggggttgcgc aatatagatg cagttgctca attgctgccc
     1621 gaccccgcaa agatgctgac tgcattaggc gtgcgcccca ccagcggtac acagaccgtc
     1681 atgccgggca tcattagcgc cttcgtaaat tttttactgg gcatggtttt tctcgatatt
     1741 ggctcattag ccgcctatgt gatcgagctc tgggcatttg atggcggcgc cattatgttg
     1801 ccgggtattc tatattttgg catgtatatc atcgccatgt ttctcatctt tggggtgatc
     1861 tacctggtga atacctcgtt gaccgcgcgc cgcggctggc tggcttcgat aaacggtttg
     1921 attttttcac aagcgttgtt atttgtattg ttctactggc aggcagcaca agttgataat
     1981 atagacgcac tttatgccca aagcggtgtg acggatccgg gcgggtactt cattatggtc
     2041 cccttcctcg tgtatatcgc aggcggtttc cttctaagtg cagcagccct gttccgcttc
     2101 agtgacattc gacgctggtt cccagccaag acaacgtgag atggcagcat attgttatgc
     2161 tggggaagtg ctataaagca aattcaactt gcgttctgat tctccc
//
LOCUS       DAOQJE010000671         2205 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_38227_length_2205_cov_7.032093, whole genome shotgun sequence.
ACCESSION   DAOQJE010000671 DAOQJE010000000
VERSION     DAOQJE010000671.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2205)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2205)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2205
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38227_length_2205_cov_7.032093"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..581
                     /locus_tag="PLT08_15035"
     CDS             <1..581
                     /locus_tag="PLT08_15035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE05838.1"
                     /translation="ANYSKPDVIRSPAMYNPGSVQRFLFWFDGWIHYLFLLFNPNQKA
                     RQIALEEMRAKAHNLFEEHVFDDLRDEEDHYVIRLPMEIATGDGLRVGFQGKNSREPM
                     LKSSSYLKRVMSDRHVSLPPIREWRCILLNSEQGWVQYKGLWGVKSILSEESGPPGPR
                     WDKPKRKKTGVSERVRWGKPLKWLALLEKNKH"
     gene            603..1505
                     /locus_tag="PLT08_15040"
     CDS             603..1505
                     /locus_tag="PLT08_15040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE05839.1"
                     /translation="MSISQALEVAIGLIFVYYVLGSIVSLITQWINESFETRGKSVER
                     HLMKIVGDKHVDEFVKLPQLQALRPIRYKNMFSFLTSSTEPKKLEKIPAATLVDSYFD
                     FVGLTSQEFKADELKDFINAFPDSEGKQAMLKWVSQGVTNIEDLRKRTTTYFSGVMDQ
                     AASTFKSNARSFVIMLSIFLTLLLGTDSIQLAQTLWANASVRAIAVAQAQAVVQQEGS
                     NANVDDLLQQLLDLQIVKIGWWQTDLPQTTDIGTWGGFIALKFLGLGLTAVAVSQGSS
                     FWYDLLKKIVSPSKGGGGGDEAKG"
     gene            complement(1546..>2205)
                     /locus_tag="PLT08_15045"
     CDS             complement(1546..>2205)
                     /locus_tag="PLT08_15045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucomutase/phosphomannomutase family
                     protein"
                     /protein_id="HNE05840.1"
                     /translation="IGAMDERGNFVDPHKIMALSLKYLVEKRGMTGAVVRTVSTTRML
                     DRLAKRYGVKIYETPVGFNHIADIMMNEDVLIGGEESGGISFKGHIPEGDGPIMGLLI
                     VEMLAAYGKSLGELVDDLLADVGPAFYERVDLRLSRPVAKAEMTEFLTKNAPAEIGGA
                     TVAEISQRDGVKYIMSDDSWLLVRPSGTEPVLRVYAEGRTQEMVKALLGYGKTVAESV
                     V"
BASE COUNT          574 a          506 c          548 g          577 t
ORIGIN      
        1 acgcgaatta cagcaaacca gatgtgatcc gttcgccggc aatgtataac ccggggagcg
       61 tgcaacgctt cctcttctgg ttcgacggct ggatccacta tcttttcctt ttgtttaacc
      121 cgaaccaaaa agcacgtcag attgcattgg aggagatgcg tgctaaagct cataacttgt
      181 ttgaagagca tgtcttcgac gatctgcggg atgaagaaga ccattatgtc atccgtttgc
      241 cgatggagat tgccacaggc gatggattgc gagttggctt tcagggcaag aactcaagag
      301 agccgatgct caagtcttct agctatttga agcgagtgat gtctgaccgg catgtgtcgc
      361 tcccgccgat tcgagaatgg cgatgtatcc tcttgaattc cgaacaagga tgggtacaat
      421 ataaaggatt gtggggtgta aaaagcatac taagcgaaga gtcgggtccg cccggaccac
      481 gatgggataa acccaaacgc aagaagaccg gtgtaagtga gcgtgttcgt tggggcaagc
      541 cgctaaaatg gcttgcgcta ctggaaaaga ataaacatta atgcaactca aaggagaaag
      601 atatgtcaat ttcacaagcg cttgaagtag ccattggctt gatctttgtt tattacgtgt
      661 taggttcgat cgtttctctc atcacacaat ggatcaatga atcgttcgaa acacgtggta
      721 aatcagtgga acgacaccta atgaagattg tcggcgataa acatgtggat gaatttgtaa
      781 aacttccgca gttgcaggca ttacgcccta ttcgttataa gaatatgttt agcttcctta
      841 catcttctac agaacctaaa aaactagaga agatccctgc tgcgactttg gttgattctt
      901 attttgattt tgttgggctc acttctcaag aattcaaagc cgatgagtta aaagacttta
      961 ttaatgcttt ccctgactca gaaggcaaac aggcgatgtt gaagtgggta agccagggtg
     1021 tgaccaatat tgaagacctg cgaaagcgta cgactaccta cttctctggt gtgatggatc
     1081 aggcggcttc aacctttaag tcaaacgctc gcagttttgt cattatgttg tctattttct
     1141 tgaccttgtt gcttggcaca gatagcattc agcttgcaca aaccttgtgg gcaaatgcga
     1201 gtgtccgtgc tattgcggtg gcacaggcac aagctgtggt gcaacaagaa ggcagcaatg
     1261 ccaatgtgga tgacctcctt caacaattgt tggatctaca aatcgtaaag atcggctggt
     1321 ggcaaacaga ccttcctcaa accacggata ttggcacatg gggcggattt attgctttga
     1381 aatttctcgg gctcggtttg accgctgtgg ctgtttcgca aggctcgtcc ttctggtatg
     1441 atttactcaa gaagattgtc tctccatcca aaggtggtgg gggtggggac gaagcgaaag
     1501 gataataaaa aagaggcacc tgttcaggtg cctctttttt attgtttaca ccacactctc
     1561 agcgacggtc tttccgtacc cgagcagggc tttgaccatt tcttgtgtgc gcccttcggc
     1621 atacacacgt agaacgggtt ctgtgcctga tgggcggacc aagagccaag agtcatctga
     1681 catgatgtat ttcacaccat cacgttgact gatctcagct actgttgcac caccgatttc
     1741 agctggggca ttcttggtga ggaactcagt catttcggct ttggcaaccg ggcggctcaa
     1801 acgcaggtca acacgttcat aaaaggcggg tcccacatct gcgagcaggt catctaccag
     1861 ttcaccaaga gacttcccgt atgcggcaag catttcaaca ataagcaagc ccatgatagg
     1921 accatcccct tcggggatgt gtcccttgaa agaaatgccg ccagattcct caccgccgat
     1981 gagaacatct tcattcatca taatatctgc gatgtgattg aaaccgacgg gcgtttcgta
     2041 gatttttacc ccataacgct tggcgagacg gtctaacatg cgtgtggtcg aaacagtgcg
     2101 gaccacggca ccggtcatgc cgcgtttctc gaccaaatac ttgagcgaaa gcgccataat
     2161 tttatgcggg tcaacaaagt tgccgcgttc atccatggca ccgat
//
LOCUS       DAOQJE010000672         2201 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_38400_length_2201_cov_4.630475, whole genome shotgun sequence.
ACCESSION   DAOQJE010000672 DAOQJE010000000
VERSION     DAOQJE010000672.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2201)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2201)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2201
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38400_length_2201_cov_4.630475"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1945
                     /locus_tag="PLT08_15050"
     CDS             <1..1945
                     /locus_tag="PLT08_15050"
                     /inference="COORDINATES: protein motif:HMM:NF014893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="bi-domain-containing oxidoreductase"
                     /protein_id="HNE05841.1"
                     /translation="LDKAKREGIMPTVQAVFNRLDQPMGLGYSTAGTIVALGKNMQGF
                     KVGQRVACAGGGYATHAEYNVVPRNLLTPLPKNVDFESASFTTLGAIALHGFRLAETQ
                     LGENVAVIGLGLLGLLTIQLAAAAGCNVLGIDIDPKRVKLASSLGIQAVTRPQAEATA
                     SAFTANRGFDSIIICADTSSNDPVELAGIIARDRAKVVATGAVGLNFPRKVYYEKEIA
                     FINSRSYGPGRYDLNYEENGQDYPIGYVRWTEGRNFQAVVDLMASGKLKVESLISHRF
                     DIEEGVKAYEVITGKKKEPFLGVLLKYSNEKQETSKRVEFPLVARSSSLGSVKLGVLG
                     AGLFANSTLLPVLKNNKDFELVGIASSGGLHAQHSGKKFGFQYATSSEDEIINDPKIN
                     TVAILTRHDTHADLIVKALKAGKNVFVEKPLAITSEQLKKIEKQLSITNYQLLVGFNR
                     RFSPLAQQLHASLVSRQEPLHAHYRVNAGFIPANHWTQDESIGGGRIIGEACHFIDLI
                     TYLVGALPVRVSAHALPNNGKYKEDNVSMTFTFADGSIGVVDYLANGDKSMPKERLEV
                     FCGGMVAVLDDYVSLTTVKDGKRKEERIAQDKGWRAEMAAFADSVKAGKEPPIPYEQI
                     IAVTKSTFAAVESIRSGNPVEIK"
     gene            1948..>2201
                     /locus_tag="PLT08_15055"
     CDS             1948..>2201
                     /locus_tag="PLT08_15055"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNE05842.1"
                     /translation="MQIVTFDGKDEGLKHQMAQLLVDAFREHWADAWETLEDGLKEVN
                     EMLETERICLVAVDGERLLGMIGGISGYDGKVWELHPLAV"
BASE COUNT          560 a          507 c          590 g          544 t
ORIGIN      
        1 gctcgataaa gccaaacgcg aaggcattat gcccacggtg caagccgtgt tcaaccgcct
       61 cgaccagccg atggggctgg gctattccac cgccggaacc attgtggctc ttggcaagaa
      121 catgcaaggc ttcaaggtcg gtcaacgggt ggcatgcgcg ggcggcgggt atgctacgca
      181 cgccgaatac aacgtcgtgc cgcgcaatct attaactcca ctgcctaaaa atgtcgattt
      241 tgaatccgcc tccttcacca ctcttggtgc cattgcatta cacggtttcc gtttggcaga
      301 gactcagctt ggtgaaaatg ttgccgtgat cggtttgggt cttttgggat tattgacgat
      361 tcaactggcg gctgcggcgg gatgcaacgt cctcggcatt gacattgacc cgaagcgggt
      421 caagctcgcc tcttctcttg gaattcaagc tgttacacgt ccccaagccg aagcgaccgc
      481 ctcagccttt accgccaacc gcggcttcga cagcatcatc atctgtgccg acacctcttc
      541 caatgacccg gttgaactag cgggcatcat tgcaagagac cgtgccaaag tggttgctac
      601 aggtgccgtg gggcttaatt ttccgcgcaa ggtgtactac gaaaaagaga tcgccttcat
      661 caattctcgt tcttatggac ccggtcgcta tgacctgaac tacgaagaga acggacagga
      721 ttaccccatc ggttatgtgc gttggacgga aggacgtaat tttcaagccg ttgtcgattt
      781 gatggcaagt ggaaagttga aggtcgaaag tcttatcagc catcgctttg acattgaaga
      841 gggtgtgaaa gcctacgaag tgattacggg gaagaagaaa gagccgtttc ttggggtatt
      901 gcttaagtac agtaatgaga aacaagaaac gagtaaacga gtggagtttc ctcttgttgc
      961 tcgttcctcg tcacttggtt ctgtgaaact cggcgttctt ggcgctgggt tatttgcgaa
     1021 ctcaacatta cttccagttc taaagaataa caaagacttt gaattggtgg gcattgcatc
     1081 ttcaggtggt ttacacgctc agcactctgg taagaaattt ggttttcagt atgccacgtc
     1141 ttcagaagat gaaattatca atgatccgaa gatcaacaca gtagctattc tcacccgtca
     1201 cgatacccat gcggatttga tcgtaaaagc actcaaagcc ggaaaaaatg tatttgttga
     1261 aaaaccgctt gccattacat cagaacaact caagaaaatc gaaaaacaat tatcaattac
     1321 caattaccaa ttactcgtcg gcttcaaccg tagattttcg ccgcttgctc aacaacttca
     1381 cgcttcactc gtctctcgtc aggaacctct gcatgcccat taccgcgtca acgcgggctt
     1441 tatccctgcc aatcactgga ctcaagacga atccatcggt ggtggacgca tcatcggcga
     1501 agcgtgtcac ttcattgact tgatcacata tctagtaggt gctttgccag tgagggtctc
     1561 tgcacatgcg ttgccgaaca acggcaaata taaagaagat aacgtctcga tgacatttac
     1621 ttttgcggac ggctccatcg gtgtggtgga ttatctcgcc aatggcgaca agtccatgcc
     1681 gaaggaacgg ttggaggtat tctgtggggg aatggtggcg gtgttggatg attacgtctc
     1741 attgaccact gtgaaagacg gcaagaggaa agaagaaaga attgcgcaag acaaaggttg
     1801 gagggcggaa atggctgcct tcgctgattc agtcaaggcg gggaaagagc caccgattcc
     1861 gtatgagcag atcatagctg tgaccaaatc cacgtttgcg gcggtggagt cgattcgaag
     1921 cgggaatccg gttgagatta agtagccatg caaattgtga cgttcgatgg aaaagacgaa
     1981 ggactcaaac atcaaatggc gcagttgttg gtggatgctt tccgcgagca ttgggcggat
     2041 gcgtgggaga cgcttgaaga tggtttgaaa gaagtgaatg agatgcttga aacagaacgc
     2101 atttgccttg tggctgtaga tggcgagcga ttgctcggca tgatcggtgg tatctctgga
     2161 tatgatggca aggtgtggga gttgcatccg ttggcggtac a
//
LOCUS       DAOQJE010000673         2195 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_38598_length_2195_cov_6.402804, whole genome shotgun sequence.
ACCESSION   DAOQJE010000673 DAOQJE010000000
VERSION     DAOQJE010000673.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2195)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2195)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2195
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38598_length_2195_cov_6.402804"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..365)
                     /locus_tag="PLT08_15060"
     CDS             complement(<1..365)
                     /locus_tag="PLT08_15060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE05843.1"
                     /translation="MSKTISAGRTPRIEITAVSGDLSLVGWEGDDILLKADDEEMNVK
                     QDGDQVTIACKDDLSMRVPKGSTVIIHRITGDASVRGVMGAIELKEISGDLSMRDVVT
                     VAIDTVHSDFSLRGAKGSLS"
     gene            complement(369..752)
                     /locus_tag="PLT08_15065"
     CDS             complement(369..752)
                     /locus_tag="PLT08_15065"
                     /inference="COORDINATES: protein motif:HMM:NF021388.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2089 domain-containing protein"
                     /protein_id="HNE05844.1"
                     /translation="MRPAPTRCPICQSEMEVVRLHCSSCDTSIEGSFALGQFSNLSPE
                     QMEFVFTFVRCEGKINRMEQELGLSYPTIRNRLHEVIRKLGFEPGRDETPEVDDETRS
                     SVLDDLESGKITADQAMRILRGEEE"
     gene            974..1960
                     /gene="corA"
                     /locus_tag="PLT08_15070"
     CDS             974..1960
                     /gene="corA"
                     /locus_tag="PLT08_15070"
                     /inference="COORDINATES: protein motif:HMM:TIGR00383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015087 - cobalt ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006824 - cobalt ion transport [Evidence
                     IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium/cobalt transporter CorA"
                     /protein_id="HNE05845.1"
                     /translation="MIRSLYYKPGQPIRTNIPPTEFPRLIRDRKGTLWVDFIGEPEEL
                     AEPILRGFGFHPLAIDDALQETHTPKVDDWGDYIYLVLSILNYKQENGMFEAEIDELD
                     VFLGHNYVVTFHDQLLTAVEDTWIACQRDIRHVQDGADHLLYRIVDSLVMSYMPLVEQ
                     IDAQIDQIEDQVFDKPRREILEQIFALKRLLLTMRRILLPQREVLNKLARDDFRVIDP
                     RDRVFFRDIYDHLVRLHDLNESLRDLVSGALDTYLSVTNNRMNEIMKVLTIITTFFMP
                     ITFITGFFGMNFFEPVAHLVGWTSQQVFAGMMSVMIGLPILMYFWMRGRTWL"
BASE COUNT          566 a          567 c          477 g          585 t
ORIGIN      
        1 gataaacttc cttttgcacc acgcaggcta aagtccgaat gaaccgtatc aattgccacg
       61 gtcactacat cgcgcataga aaggtcacca gagatttcct ttaattcaat cgctcccatc
      121 accccacgca cagaagcatc acccgtaatg cggtgaataa tgacggtcga ccctttgggg
      181 acgcgcatcg aaagatcatc tttacaagca atagtgacct gatcgccatc ttgcttgacg
      241 ttcatttctt cgtcatctgc cttgaggaga atgtcatctc cctcccaccc taccaagctc
      301 agatcaccac tgactgctgt aatttcgatc cttggggtgc gtcctgcaga aatggtctta
      361 gacatggctt attcctcctc gccgcgcaaa atgcgcatgg cttgatctgc tgtgattttc
      421 ccggattcga gatcgtcgag aacactgctg cgggtttcat catccacttc aggggtctca
      481 tctctgccgg gctcaaaacc aagtttgcga atcacctcgt gcaagcggtt gcgaatggtt
      541 gggtatgaaa gccccaactc ctgctccatg cgattgatct tgccttcgca gcgcacaaag
      601 gtgaaaacaa attccatctg ttcaggagag agattcgaaa actgccccag agcaaagcta
      661 ccttcaattg aggtatcgca gctggagcag tgcaaacgaa caacttccat ttcagattgg
      721 cagatcgggc aacgggttgg ggcaggtcgc ataacaggct tctccgattt ataatgtgtc
      781 aaaaagtaat tttttagccg aaaatcgata tttttaagga cttttttcaa aaaatcaata
      841 aatttatcct gatttctaat aaaattatac aacagattta acttttgtca atagatttta
      901 cagaaaatca atatcaactt gaagattatt gagtttagct ttaaaatatt aaagtcgtcc
      961 ctggaggtca ctcttgattc gctctctcta ctacaaacct ggtcagccca ttcgcacaaa
     1021 catcccccct actgagtttc cgcgcctcat acgcgaccgg aaaggcactc tttgggtaga
     1081 tttcattggg gagcctgaag agctagcaga gcccattctg cgcggttttg gcttccatcc
     1141 gctagccatt gacgatgccc ttcaagagac ccacaccccc aaagtggacg attggggcga
     1201 ttacatctac cttgtgttga gcattttgaa ctacaagcaa gaaaatggaa tgtttgaggc
     1261 agaaattgac gaattggatg tattccttgg gcataactat gtggtcactt ttcatgacca
     1321 gttattgacc gcggtagaag atacttggat cgcctgtcaa cgagatattc gccatgtgca
     1381 agacggagca gatcacctgc tctatcgcat cgtcgactcg cttgtgatga gctacatgcc
     1441 gcttgtagag caaattgacg cccaaattga ccaaattgag gatcaagtct tcgacaagcc
     1501 gcgacgtgag atccttgagc agatttttgc tctcaaacgc ctccttctaa cgatgcgccg
     1561 gatcctgctc ccccagcgtg aagtgctgaa caagcttgcc cgcgacgact tccgcgtgat
     1621 cgaccccaga gaccgtgtct tcttccgcga tatttacgat catctcgttc gcctgcatga
     1681 cctcaacgag agcttacgtg accttgtcag cggcgcgttg gatacctatc tttcggttac
     1741 caacaaccgc atgaatgaga tcatgaaagt cctcacaatt attacaacct ttttcatgcc
     1801 tattaccttc atcaccggct ttttcggcat gaatttcttc gaacctgttg cccaccttgt
     1861 gggttggacc agccaacaag ttttcgccgg catgatgtca gtcatgatcg ggcttcccat
     1921 cctgatgtat ttttggatgc gtggacggac ctggttatag ctccattttg caccccaaaa
     1981 tgccttgaca cagaacaggc gttcggttat cattattact agcacaaatg agcgaagaat
     2041 gctctgtacc tacgccagtc ccctggcagg aggcttgaaa tgatgatggt tcgaaaaagt
     2101 aaaggtatcg gcgaacgcca ccaaaaaatt ctggatttta ttgcgtctta ccagcgcgaa
     2161 cacaagcacc ccccttccat tcgtgagatt gggga
//
LOCUS       DAOQJE010000674         2195 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_38599_length_2195_cov_5.503271, whole genome shotgun sequence.
ACCESSION   DAOQJE010000674 DAOQJE010000000
VERSION     DAOQJE010000674.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2195)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2195)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2195
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38599_length_2195_cov_5.503271"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..844)
                     /locus_tag="PLT08_15075"
     CDS             complement(<1..844)
                     /locus_tag="PLT08_15075"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HNE05846.1"
                     /translation="MSTLTLASEVAMKRLLILTVGLLVLTACASPTPTPVPDAVETTA
                     PVIEVSSPDVVIASADVEPTHVSHLGFTISALVKEVAVGEGDVVKAGDTLMVLNTPDL
                     QYAVVSAEADFNARTQAAQLQKADKVLYVNPNTGKKTWYSLPREVFLKAQAQADQAKA
                     VWDTAAATYAQSTLTAPFDGVVVDVAVIPGELVQVNQVVITIADLNDLQITTTDLSER
                     DIARVKLGQSVNVSIEALGQTISGKVIRISPVSETVGGDVVYPVTIQLDEQPDGLMWG
                     MSAEV"
     gene            complement(819..1652)
                     /locus_tag="PLT08_15080"
     CDS             complement(819..1652)
                     /locus_tag="PLT08_15080"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HNE05847.1"
                     /translation="MKRVIFVVVSLLALLLSACGQASSTEAVPTVVIDAGNSSQPADS
                     QPSSGGSVSASAIVVPMQDANLSFTNIGRVTQVNVQVGDKVSAGDVLVQLDTTILEAK
                     VREAEANLKYAQISLDYLIRNVGCKMNCAPSQEHIEVAENDVAKAQALVDSAKAVLAA
                     QSNLTAPFAGTVISVDIAPYETVAPGQVVMVIGDLSKYQIETTDLSERDVTQVKVGQP
                     ASVYIEALDEEFTGKVVDVARISSEVGGDVVYKVTIEFDKQPAGLLWGMSADVNIDAG
                     E"
     gene            complement(1741..>2195)
                     /locus_tag="PLT08_15085"
     CDS             complement(1741..>2195)
                     /locus_tag="PLT08_15085"
                     /inference="COORDINATES: protein motif:HMM:NF024700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNE05848.1"
                     /translation="QRADMRKGHIGFIFQFFALIPTLTAYENVEMPLLLNGHSPKERR
                     DRVMELLKAVDLTDRANNRPDQLSGGQQQRIAIARALAPKPALILADEPTANLDTENG
                     KQVMEIMKKLNQETGETFVFATHDPRVINYASRVVTLEDGVIVKDSKG"
BASE COUNT          561 a          575 c          528 g          531 t
ORIGIN      
        1 ctacttcagc gctcattccc cacatcaagc catcgggttg ttcatccaat tggatagtca
       61 ccgggtagac cacgtcaccg cctacggttt cagaaaccgg tgaaatgcga atgacttttc
      121 ctgaaatggt ctgtcccaac gcttcgatgg aaacgttcac gctctgcccg agttttacac
      181 gcgcaatatc gcgttcgctc aagtctgtgg tggtgatttg gaggtcgttc aagtctgcta
      241 tggtgataac gacttgattc acctgcacca actcacccgg gatgaccgca acatccacaa
      301 ccacgccatc aaagggagct gttagggtgg attgagcata tgtagcagcg gcagtgtccc
      361 aaacggcttt tgcttggtcg gcttgggctt gggctttcaa gaacacttca cggggcaagc
      421 tataccaagt tttcttgccg gtgttcgggt tgacatacaa aaccttatcg gctttctgaa
      481 gctgcgctgc ctgcgtgcgc gcattgaaat ctgcttcagc agaaaccacg gcatactgaa
      541 ggtctggggt attcaagacc atcaacgtat cgccagcctt aaccacatcc ccttcaccaa
      601 cggcaacttc tttaacaagt gccgagatag taaacccaag gtgagaaacg tgagtcggtt
      661 cgacatcagc agaagcaatc accacatctg gcgaagacac ttcaatgacc ggggcagttg
      721 tttcaacggc atcaggaaca ggcgttgggg ttgggctggc gcaagccgtt agcactagca
      781 agccaacggt aaggattaac aatcgcttca tggcaacctc actcgccagc gtcaatgttg
      841 acatccgcgc tcatgcccca caataagcct gcaggctgtt tatcaaattc aatggtcact
      901 ttgtagacca catcgccgcc aacctctgaa gagatgcgtg ccacgtccac aactttgccg
      961 gtgaattctt cgtccaacgc ttcgatgtat accgaagcag gctgacccac ttttacctga
     1021 gtcacatcgc gttcgctcaa gtcggtagtt tcgatctgat acttggagag gtcaccgatc
     1081 accattacaa cctgtcccgg agcgaccgtc tcatacgggg cgatatcgac cgagataacg
     1141 gtaccagcaa acggcgcggt gagattcgat tgagcggcga gaacagcctt ggctgaatcc
     1201 accaaagctt gagccttggc aacgtcattc tctgctactt caatgtgttc ctgtgaaggt
     1261 gcacaattca tcttacagcc cacattgcga atcaaataat cgaggctgat ctgcgcgtat
     1321 ttcaaattgg cttctgcctc gcgaaccttg gcttccaaaa tggtggtatc caactgaacc
     1381 aacacatcac ctgcgctaac cttatcacca acctgcacat tgacctgagt cacacgtcca
     1441 atattggtaa aggaaagatt ggcatcttgc atgggaacaa caatcgccga agcagaaact
     1501 gaaccgccgc ttgaaggttg agaatcggca ggctgggatg aattgcccgc atcgatcaca
     1561 acggtgggaa cggcttctgt tgaactagcc tgtccacacg cggacagcaa aagagccagc
     1621 aaactaacaa ctacaaaaat aactcgtttc attctttatt tctccaaact gtgtaatcct
     1681 tgaagtttct aaagtatatc atattgcaca gtttttgcaa aggttttgtc aagaaaggac
     1741 ttatccctta gaatccttga caatcacccc atcttccaaa gtcaccacac gtgaagcgta
     1801 attaatgaca cgcggatcat gtgtagcgaa gacaaaggtc tcccctgttt cttgattcag
     1861 cttcttcatg atctccatca cctgcttgcc attctcggta tcgaggttgg cggtcggctc
     1921 atcggcgagg atgagagcag gtttgggtgc caaagcacga gcaatggcga tgcgttgttg
     1981 ttgaccgcca gagagttgat cggggcgatt attggcgcga tcggttagat ccacggcttt
     2041 gagcaattcc atcacacggt cgcggcgttc ttttggagaa tgcccattga gcagaagcgg
     2101 catttccacg ttttcatagg cagtgagggt ggggatcaaa gcaaagaatt ggaagataaa
     2161 cccaatatga cctttgcgca tgtcggcacg ctgat
//
LOCUS       DAOQJE010000675         2192 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_38672_length_2192_cov_5.139916, whole genome shotgun sequence.
ACCESSION   DAOQJE010000675 DAOQJE010000000
VERSION     DAOQJE010000675.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2192)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2192)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2192
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38672_length_2192_cov_5.139916"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..206)
                     /locus_tag="PLT08_15090"
     CDS             complement(<1..206)
                     /locus_tag="PLT08_15090"
                     /inference="COORDINATES: protein motif:HMM:NF024489.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE05849.1"
                     /translation="MTEIDLSHPSLYINREASLLEFQRRVLEEARDEENPLLERVKFL
                     AILGSNMDEFFMVRVSGIRKQVEA"
     gene            complement(257..757)
                     /gene="sixA"
                     /locus_tag="PLT08_15095"
     CDS             complement(257..757)
                     /gene="sixA"
                     /locus_tag="PLT08_15095"
                     /inference="COORDINATES: protein motif:HMM:TIGR00249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0101006 - protein histidine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohistidine phosphatase SixA"
                     /protein_id="HNE05850.1"
                     /translation="MNLYLIRHAIAEEESPAVEDRLRALTEKGSKKMRNIAKGLKTLG
                     LEFDYIISSPYVRAQQTAEILGDVFKLRKRIVISESIAPMGNPSQLLAEINEKYTVNS
                     LAIVGHEPYLSNLISLLTAGGAPLEMTFKKGGVCYLTTDDLHHTRKATLQWLLTPGVL
                     VEIGEG"
     gene            1228..1935
                     /locus_tag="PLT08_15100"
     CDS             1228..1935
                     /locus_tag="PLT08_15100"
                     /inference="COORDINATES: protein motif:HMM:NF014826.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HNE05851.1"
                     /translation="MGLADLHLHTIYSYDGTATVPAVLRRARELGLNVIAITDHDEIA
                     GALEAVKLASHYGVEVIPGSEITTAEGDLLALFIHEKIQPGLSLIETLLRVKEQGGVA
                     IAAHPMAGGMGMKSLTPASILKALKHPIAKKVLIGIETYNGTAIDRVSNHYTRIFAQG
                     LKIAHTGNSDAHIIDTIGFGATEFPGTTAADLLNALRERTTRVRKMNEWSAFRVMSSW
                     GFRYIESAFVRLKLVRA"
     gene            1993..>2192
                     /locus_tag="PLT08_15105"
     CDS             1993..>2192
                     /locus_tag="PLT08_15105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HNE05852.1"
                     /translation="MTKRILVTNDDGVLAPGLLALAKEMRNLGDVTILAPDRNWSGGG
                     HVKTLDRALRVREFRLEDGSQA"
BASE COUNT          563 a          591 c          490 g          548 t
ORIGIN      
        1 ttggcttcca cttgtttgcg gatgccagag acacgcacca taaagaactc gtccatattc
       61 gagccgagga ttgccagaaa cttcacgcgt tcaagcagcg gattttcttc atcgcgcgct
      121 tcttctagga cgcggcgttg gaactctaat aggcttgctt cacggttgat atataaagaa
      181 ggatggctca ggtcgatctc ggtcattagg ttttcctcaa ggaaggatgg gcgtgcaaaa
      241 gaatccctgt attctattat ccttccccaa tttcaaccag cacaccgggt gttaatagcc
      301 attgcagggt tgctttgcgg gtgtgatgca agtcatctgt cgtaaggtag caaaccccgc
      361 ctttcttaaa ggtcatttca agaggagcgc cgccagctgt tagtaggcta ataagattcg
      421 acagatacgg ctcatgccca acgatcgcaa ggctattgac cgtatatttt tcattgatct
      481 ccgccaataa ctggctcggg tttcccatag gagcaatgct ttcgctaatc acaatgcgct
      541 tcctaagctt aaagacatcg cccaaaatct ctgccgtttg ttgagcgcgg acgtatgggc
      601 tgctaatgat gtagtcaaac tccagcccaa gggtcttaag ccccttggcg atgttgcgca
      661 tctttttgga gcctttctcg gtcaacgcgc gcagacggtc ttctaccgca ggcgattctt
      721 cttcggcaat ggcatggcgg atcagatata aattcatagg gcgctctcct gaaaataaag
      781 tactcgaatt atacaggctg caaaattttc ctcattaaga atatgtaaat aaatctgtac
      841 aggtggtttt cgcctccgcc ttgcataatc gtttacccgt tgttaactgg ttcttaagtc
      901 caaaatcaaa tgactgttgt aagatacagg catggaaacg tacttcgctc ttttcttatt
      961 tctctctttg ttgaccgtct tcacaataga cgcttctcgc aaacgtcgcg agtttttgca
     1021 aagacaaagc cgcgcgtagt tctttttcat ttttcttctc ctccttcctt catccacccg
     1081 cctgacagcg acaggcgggt ggaatcgtta acaaaaacca cacaacaagt tcaactccca
     1141 ttaacaactc cgcactacac tacaactata ttcttgaaac tttccgaacg catttcggtc
     1201 taaagagata tagaaacgga gaatacaatg ggacttgcag acctacactt gcatacaatc
     1261 tacagctacg atggcaccgc caccgtccct gctgtattac gccgcgcacg agaactcgga
     1321 ttaaatgtca tcgccattac ggatcatgac gaaattgctg gcgcgttgga agccgtcaaa
     1381 ctcgcctcac actacggcgt ggaagtgatc cccggaagtg agatcaccac cgctgaaggc
     1441 gatttgctcg cgctcttcat ccacgaaaaa attcaaccag gactatccct cattgaaacc
     1501 ttgttgcgtg ttaaagaaca aggcggagta gcaattgccg ctcaccctat ggcaggcggc
     1561 atgggcatga aaagcctgac ccctgcgtcc attttgaagg cgcttaagca tcctatagct
     1621 aaaaaagtgt tgattggtat tgagacgtat aacggcaccg ccattgaccg ggtaagcaat
     1681 cactacactc gcatctttgc ccaagggttg aaaatagccc acaccggcaa cagtgacgca
     1741 cacatcatcg acaccatagg cttcggcgcg acagaattcc ccggcaccac cgccgcagac
     1801 ttgctcaacg ccctgcgcga acgcacaacc cgcgtccgca aaatgaacga atggagcgct
     1861 tttcgcgtga tgagtagttg gggttttcgc tatattgaaa gcgcttttgt ccgcctaaag
     1921 ttggtgcgtg cataaatctt ctcttatctt tcatcattac aataggggaa actatataaa
     1981 gagacaacaa ccatgaccaa acgaatcctt gtcaccaacg acgacggcgt acttgccccc
     2041 ggcttgctcg ccctcgccaa agaaatgcga aacctcggtg atgtgaccat cctcgcccca
     2101 gaccgaaact ggtcgggcgg cggtcatgtc aaaaccctag accgtgccct gcgcgtgcgc
     2161 gagttccgcc tcgaagacgg ctcacaagcc tt
//
LOCUS       DAOQJE010000676         2189 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_38764_length_2189_cov_6.350515, whole genome shotgun sequence.
ACCESSION   DAOQJE010000676 DAOQJE010000000
VERSION     DAOQJE010000676.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2189)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2189)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2189
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38764_length_2189_cov_6.350515"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..771
                     /locus_tag="PLT08_15110"
     CDS             <1..771
                     /locus_tag="PLT08_15110"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-transferase"
                     /protein_id="HNE05853.1"
                     /translation="NLTLARATPDLIYDQLVAAGCAKKVIFSYMGNPGVGSLRIVRGA
                     IERGELEWEEYSHFGMITRLQAGASGLPFLPMNQTGAEDLEKVNPQIKRILDPYGGKD
                     VIVVPALNPDVAIVHVQRADKNGNAHLWGIIGEQKEAAFAAKKVILTAEEIVDETVIR
                     SDPNRTMIPGIVVSAVCHVPFASHPSYSQGYYDRDNEFYLAWDKISESKELTQKYLDE
                     WVYGVKDRNAYWKKLGEKTHKRLKVKAQYSEKINYGKY"
     gene            773..1621
                     /locus_tag="PLT08_15115"
     CDS             773..1621
                     /locus_tag="PLT08_15115"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HNE05854.1"
                     /translation="MNPIPHFDFGGNGEPLHFLHANGYPPECYTSLLEHLKHNYHLFG
                     MKLRPLWDGANMDDLHDWHPYSEDLLRLLSDRETGPVIGVGHSIGGIVTLRAAMREPK
                     RFRAVILLDPVLFVPSTMNIWNFVRAIGIGEKAHPLIQTAKRRRREFSDLESVFRSYR
                     TKPVFRYMSDESLKQYIVGITKPKASDGYELVYTPEWETHIYMTGMRDYDLWRGLANF
                     DVPTLIIRGDETDTFLPNAEKLVKKKNPKIQIHTMKKATHILPLEHPKEVADIIKNFL
                     KPDNRP"
     gene            1666..>2189
                     /locus_tag="PLT08_15120"
     CDS             1666..>2189
                     /locus_tag="PLT08_15120"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-transferase"
                     /protein_id="HNE05855.1"
                     /translation="MPELKYSSAELMIINAARLLRDGDVVFVGVGQPNLACNLAKRTH
                     APNLVMIYEAGVIGAEPERLPLSIGDPTLVSGALSVVSMYDIFANYLQRGNVDVGFMG
                     GAQIDKYGNINATTIGGYAQPKVRLPGSGGSQEIAAWANRCYIMTPHQKRRFPEKVEF
                     MTSAGFIDGKGAREA"
BASE COUNT          595 a          527 c          528 g          539 t
ORIGIN      
        1 aacctcaccc tcgcacgcgc caccccagac ctgatctacg atcaactggt tgcggcgggt
       61 tgtgccaaga aagtgatttt ctcctatatg ggaaatcccg gcgttggttc gttacgcatc
      121 gtgcgtggcg ccattgagcg tggagagttg gaatgggaag aatattctca cttcggcatg
      181 attactcgct tacaggcagg tgcatctggg ttgccattcc tgcccatgaa tcaaaccggt
      241 gcagaggact tggaaaaggt taatccgcag atcaagcgca tcctcgaccc atacggcggc
      301 aaagatgtga ttgtcgtgcc agccttgaat ccagatgttg ccattgtcca cgtacagcgc
      361 gcagataaga atggcaatgc ccatctttgg ggcatcatcg gcgaacagaa agaagccgct
      421 ttcgccgcga aaaaagtcat ccttaccgct gaagaaattg tcgacgaaac agttatccgg
      481 tctgacccga accgcacgat gattcccggc attgtcgtca gcgcggtatg tcacgttccg
      541 ttcgcgagtc atccatcgta ttcgcaaggt tattacgatc gcgacaatga gttctacctt
      601 gcgtgggata agatcagcga atcaaaagag ctgactcaaa aatatcttga tgaatgggtg
      661 tatggcgtaa aagaccgcaa tgcctattgg aaaaagctcg gcgaaaagac acacaagcgt
      721 ttgaaagtta aagcacaata tagtgaaaaa ataaattacg gcaagtacta acgtgaatcc
      781 aattccacat tttgattttg gcggcaatgg cgaaccgctg cattttcttc atgccaatgg
      841 ctacccgcca gaatgttaca catcactact ggaacattta aaacataact atcatctctt
      901 tggaatgaaa ctccgtccgc tatgggatgg cgccaatatg gatgacctgc acgattggca
      961 cccatactca gaagatctac tgcgcctctt atcggaccgg gaaactggtc ctgttattgg
     1021 agtgggtcat tccatcggag ggattgtcac cctgcgagct gccatgcgtg agccaaagcg
     1081 attccgcgct gtgattctgc ttgaccctgt gcttttcgtt cccagcacaa tgaatatctg
     1141 gaattttgtc cgcgccatag ggattggaga aaaagcccat ccgcttattc aaacagccaa
     1201 acggcgcaga cgtgaattct cagatttgga gagtgtcttt cgcagttatc gaacaaagcc
     1261 cgtttttcgc tatatgagcg atgaaagttt gaagcaatac attgtaggca tcaccaagcc
     1321 caaagcaagc gacgggtatg agctcgttta tacccctgaa tgggaaactc acatttatat
     1381 gaccggaatg cgcgattacg acctctggcg cgggcttgca aattttgatg taccaacatt
     1441 gattattcgt ggtgatgaaa ccgatacctt cctgccaaat gctgaaaagt tggtgaaaaa
     1501 gaaaaacccg aagattcaaa ttcacaccat gaaaaaagcc actcacattc tgccgcttga
     1561 acatccaaaa gaagtggctg acattattaa gaactttttg aagccagaca atagaccata
     1621 gaatgcagaa gagccattat ctattgtcaa atctaggaga acaacatgcc tgaacttaag
     1681 tattcttccg ccgaattgat gatcatcaat gccgcccgcc tcttgcgcga cggcgatgtg
     1741 gtcttcgttg gtgtaggtca acccaattta gcttgtaatc ttgccaagcg tacccatgct
     1801 ccgaaccttg taatgatcta tgaagccggc gtgatcggtg ctgaaccaga acgcctgccc
     1861 ctctccatcg gtgacccgac gctggtcagt ggtgcgcttt cggtggtgag catgtatgat
     1921 atttttgcca attaccttca gcgcggaaat gtcgatgtag gttttatggg tggcgctcag
     1981 attgataaat atggaaacat caatgccaca acaattggtg gctatgcgca gcctaaagtc
     2041 cgcttgccgg gttcgggtgg ttcacaagaa atagcagcat gggcaaaccg ctgttacatt
     2101 atgactccgc accagaaacg ccgtttccct gaaaaagtag aattcatgac ctcggctggt
     2161 ttcattgatg gcaaaggtgc gcgtgaagc
//
LOCUS       DAOQJE010000677         2188 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_38795_length_2188_cov_5.842944, whole genome shotgun sequence.
ACCESSION   DAOQJE010000677 DAOQJE010000000
VERSION     DAOQJE010000677.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2188)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2188)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2188
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38795_length_2188_cov_5.842944"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..727)
                     /locus_tag="PLT08_15125"
     CDS             complement(<1..727)
                     /locus_tag="PLT08_15125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNE05856.1"
                     /translation="MNTHPTTSQYFDFRDALHENRLLGLWRMMTDFRLSYIAATISLA
                     FSALAKTSVYLLLRFFADDVLVQGKMIGSTMTQTFLWIGLGFIGLAFIEGGGAFLSGR
                     LAAYTAEGITRRLRDFLFDHIQRLSFSYHSATPTGDLIERVTSDVDALRRFFSEQAIG
                     VGRIVLLFAINWAAILYINVKLGLVSVVTIPLILWVSLWFFKKVTKAYEDYQAQEAVL
                     STTLQENLTGVRVVKAFARQDYEM"
     gene            1058..1429
                     /locus_tag="PLT08_15130"
     CDS             1058..1429
                     /locus_tag="PLT08_15130"
                     /inference="COORDINATES: protein motif:HMM:NF024247.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HNE05857.1"
                     /translation="MTHVITSLCLRDNGCSSVCPVESINPGNPAQGWYTYYIDPSSCI
                     DCGACVSECPFHAIYPADEIPQAYKAKGGELINMAGLNGHYKGMSHTGEVIRLDSTRA
                     LTAGEVMDLTPAIALNEKFYK"
     gene            complement(1433..>2188)
                     /locus_tag="PLT08_15135"
     CDS             complement(1433..>2188)
                     /locus_tag="PLT08_15135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE05858.1"
                     /translation="VIENQAPVAKMPATVRANAPMPKRKTWLWVVLVLLLLGFGGFYF
                     LRNGFNLPALPSQSTATTESAAPLSTPQPVLPTDTPVPSPTFTATETIVPPTATIEPS
                     PTIVLTPTVKYPEGANYTLFYNETSLFIINRSYERRSISGFKFQNLNADGTLGEEVFD
                     GYRWETRNVDYLPRNFCANISIFGDEDPPYLNPVDCQQYGSIQTVQTRFDRPEKILFW
                     VPKGGTTQFVVFWLNEEVARCEITAGICEVYIP"
BASE COUNT          619 a          468 c          572 g          529 t
ORIGIN      
        1 ccatttcgta gtcctgccgg gcaaaggctt tcaccacacg aaccccagtc aggttctctt
       61 gcaacgtagt agagaggacc gcctcttgcg cctgataatc ttcataggct tttgttacct
      121 ttttgaagaa ccacaaggaa acccacaaaa taagcggaat ggtcactaca gaaaccagcc
      181 ccaacttcac gttaatgtac aagatcgcag cccaattgat cgcaaatagc agcacgattc
      241 tgccgacgcc gatggcttgc tccgaaaaga agcgccgtaa ggcatccaca tctgaagtga
      301 cgcgttcgat caagtctccg gtgggtgtgg cgctgtggta cgagaagctc aagcgttgaa
      361 tatggtcgaa gagaaagtct cgtaaacggc gggtgatgcc ttcggctgtg taagcggcta
      421 aacgtccgga aagaaacgcg ccgccgcctt caataaaggc gagtccaata aatcccagcc
      481 caatccatag gaaggtttgg gtcatggtag agccgatcat cttcccctgt acaagaacat
      541 catctgcaaa aaagcgcagg aggaggtata cagatgtctt tgccagggca gagaaggcta
      601 gtgagatcgt tgcagcaata taggacagac gaaaatctgt catcatacgc cacagaccga
      661 gcaggcggtt ttcatggagc gcgtctcgga agtcaaagta ttgggaggtg gtagggtggg
      721 tattcatggt gtttctccga acgtaacgaa aaacgattaa ataaaaaggc tgcggcgcgt
      781 gcgccacagc ccaaaaacga caaggaactg aaagagacct agggacggtc aacaggcgca
      841 ctccaagaag gcaatttgcc aactgcattg agagtttgaa tgaagattgt gttgaacatc
      901 aagagtgcgc tcctttcttt gtagatttga attacattgt gagtttacac ctctcgtttg
      961 aaaaatgtca aggttaagtt acggattaga aattaatttt tccgataact tttataacta
     1021 gtaagcgact ggatgaatat atcaaaagga gcctaaaatg acccatgtaa ttacaagcct
     1081 atgtttgcgt gacaatggat gttcatctgt ttgcccagtg gagtccatca acccagggaa
     1141 tcccgctcaa ggctggtaca cctactatat tgacccgtcc agttgtattg actgtggtgc
     1201 ttgtgtgtca gaatgcccgt tccatgcaat ttacccggcg gatgaaattc ctcaggcgta
     1261 taaagcaaaa ggcggggagc ttatcaatat ggcaggactc aatggtcatt acaaggggat
     1321 gagtcataca ggcgaagtca ttcgtttaga ctctacccgt gctttaacag caggtgaagt
     1381 catggacttg acacctgcaa tcgcattgaa cgagaaattt tataagtaaa gatcagggaa
     1441 tatagacttc acaaatgcct gctgtgattt cgcagcgggc aacttcttca ttaagccaaa
     1501 agaccacaaa ttgcgttgtg ccgcctttag gaacccaaaa gaggattttt tctgggcggt
     1561 cgaagcgggt ttgtacggtt tgaatagaac cgtattgctg gcaatctaca gggttgagat
     1621 aaggtggatc ttcatcgcca aaaatgctga tatttgcgca gaagttacgt ggtagataat
     1681 ctacgttacg cgtctcccaa cgatagccat caaaaacttc ttcgcctaag gttccatcgg
     1741 cattgaggtt ttggaacttg aacccagaaa tactgcgccg ttcatacgaa cggttgataa
     1801 taaacaaact ggtctcgtta taaaacaggg tgtagtttgc gccttccggg tacttcactg
     1861 taggcgttag aacaatcgtt ggcgatggtt cgatcgtagc ggtgggagga acaatcgttt
     1921 ctgttgcggt aaaggtaggg gaggggacgg gggtatcagt tggcagaaca ggttgaggcg
     1981 tagaaagagg cgcggcggat tctgttgtag ctgtcgattg agaaggcaac gctggcaggt
     2041 tgaatccgtt tcgaaggaag tagaaaccgc cgaatccaag aagtagtaaa accaagacga
     2101 cccaaagcca agttttgcgc ttgggcatgg gtgcatttgc gcgaacagta gcgggcatct
     2161 ttgcaacagg tgcttgattt tctattac
//
LOCUS       DAOQJE010000678         2187 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_38831_length_2187_cov_7.746717, whole genome shotgun sequence.
ACCESSION   DAOQJE010000678 DAOQJE010000000
VERSION     DAOQJE010000678.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2187)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2187)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2187
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38831_length_2187_cov_7.746717"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            286..558
                     /locus_tag="PLT08_15140"
     CDS             286..558
                     /locus_tag="PLT08_15140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE05859.1"
                     /translation="MQTPATPTQYAPCPQCGQSNAKPVNYTWWGGAIGPRMMSHVKCQ
                     SCNTQYNGKTGKSNTQNIIIYNLVVFVVVFCACGGFAVLTSILNNQ"
     gene            670..861
                     /locus_tag="PLT08_15145"
     CDS             670..861
                     /locus_tag="PLT08_15145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010266056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HNE05860.1"
                     /translation="MTMKITEDCIACGACLPDCPTESITEGDGITYVIDQATCVECEG
                     HFDSPKCASVCPVECIVKA"
     regulatory      906..1066
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            1238..2014
                     /locus_tag="PLT08_15150"
     CDS             1238..2014
                     /locus_tag="PLT08_15150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNE05861.1"
                     /translation="MKPKTLHTLSRWLGIASLLLAIPLWALIVRISGLPKFILPSPAD
                     VWMRFIKALSDGSLLYHTWVTLAEILLGLIVGVCFATIVGYLLAKSRTLETVLSPYLV
                     ASQAIPVVAIAPLLVIWLGDGILSKVIICALIVFFPVLVNTIVGVRAVSASLYDLMNS
                     LRATRWQILWKLELPASLPILFGGLRIGATLSVIGAIVGELVDAQEGLGFLLQLGDFQ
                     YDTSMVFVAVFMLVFLALCLYGVVTLLERRFLKWQQRSNV"
BASE COUNT          551 a          561 c          474 g          601 t
ORIGIN      
        1 gacccgcctc gaaaaagaac tcaaagaagc tgagtctcat attcaacgct tggagaatct
       61 gctcaatggc gacttcgcca acaaagctcc tgccgcactc attcaaaaag agcgcgataa
      121 acttgccgca tacaaagata cagcagagaa gatcaaagca caattgaagt aataaaatga
      181 ggtcacggtt gaaccgtgac ctcatttttt cataccaatg gttgattgct caatctctct
      241 tgtcttataa aataaggcta tatcttacac tcaggagaaa atctcatgca aaccccagct
      301 accccaaccc aatacgcccc ctgcccccaa tgtggacagt ccaatgcaaa accggtcaac
      361 tacacctggt ggggcggtgc cattggtccg agaatgatga gccatgtcaa atgccaaagc
      421 tgcaatacac aatacaatgg caagaccggc aaatccaata cccaaaacat tatcatctac
      481 aatcttgtcg tattcgtggt tgttttctgc gcatgtggtg ggtttgcagt tttaacttct
      541 attctcaata atcaataagc aacgcatgac aaactctcca tgaattggag atgttcgtca
      601 ctaaaacttc tgtgatgctt catgtacagt tacatactgc aagaaaaaat ccattcagga
      661 gaaactccca tgaccatgaa gatcacagaa gattgcatcg cctgcggcgc atgcctgccc
      721 gattgcccca ccgaatccat caccgaaggc gatggcatca cctatgttat cgaccaagcc
      781 acttgtgtcg aatgtgaagg acacttcgac tcacccaagt gcgcctccgt ttgcccggtg
      841 gaatgcatcg tcaaagccta attgaatcct aatcttgttg agggtataat cacctcaact
      901 gaatattcaa ccttcggggc agggtgacac gcaacccgcg tgaattcccg atcggtggta
      961 cagcccacga gcctgtttag gtacatcgct ttcctcattg ggaaaacggg catgatccgg
     1021 cgcaactccg gagccgacag tatagtctgg atataagaag gtataagcac aaccccttgt
     1081 gggatctgtg tggattctca ccccgaaaac gctctttgtt ttcggggttt ttatttatgg
     1141 aattcacgcg cctcacgcct ggattgttgc ctgttttcac gccccggagt tttatgtact
     1201 ctgaggcgtt tttatttgcc acctgtgact gatgatcatg aaaccaaaaa ctctgcacac
     1261 cttatctcgc tggctaggga ttgcctccct tctgcttgcg attccgctct gggcgcttat
     1321 cgttcgcatc agcggtcttc ccaaattcat cctgccctcc cccgctgatg tatggatgcg
     1381 tttcattaaa gcactgagtg acggcagttt gctttatcac acatgggtta cactggcaga
     1441 aattttgcta ggcttgatcg ttggcgtttg ttttgccacg atcgttgggt atctcctcgc
     1501 taaatcgcgg acacttgaaa ctgtcctttc accgtattta gtagcgagtc aggcaattcc
     1561 cgttgtagcc attgctccct tattagtcat ctggctcgga gatggcattc tctcaaaagt
     1621 tatcatctgt gccctaatcg tattctttcc agtgttggtc aatacgattg tcggggtacg
     1681 agccgtatcc gcttccctat atgacttgat gaactccctc cgtgctaccc gttggcaaat
     1741 cttatggaag ctcgaattgc cagcctcgct tcccatcctc tttggcggac ttcgcatcgg
     1801 cgcaacgtta tcagtcattg gtgcaattgt cggtgagctc gttgatgcgc aagaaggtct
     1861 gggcttcctg ttacaactcg gcgattttca atacgacacg tccatggtct ttgtagcagt
     1921 ctttatgttg gtctttctcg cgctgtgctt gtatggcgtt gtcaccctct tagaaagaag
     1981 atttttgaaa tggcaacaac gttccaacgt ataaacgttt gaacttgaaa acatcttagg
     2041 agacattgta atgaagaaag tagttttact catgctaacg ctgacagtcg ctctaatggg
     2101 ctgtaccagc tcacagtcca cgaatgcggc gggggaactt cgtcatatta aattgccgat
     2161 gggctatatt cccaacatcc aatatgc
//
LOCUS       DAOQJE010000679         2187 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_38844_length_2187_cov_3.745310, whole genome shotgun sequence.
ACCESSION   DAOQJE010000679 DAOQJE010000000
VERSION     DAOQJE010000679.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2187)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2187)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2187
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38844_length_2187_cov_3.745310"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..449
                     /locus_tag="PLT08_15155"
     CDS             <1..449
                     /locus_tag="PLT08_15155"
                     /inference="COORDINATES: protein motif:HMM:NF014801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FGGY-family carbohydrate kinase"
                     /protein_id="HNE05862.1"
                     /translation="EDKTLRAGLYNLSLNSTREDIIRAFLEGIAYNTRWLLSPVEKFL
                     GRKVNSINIVGGGAQSDLWCQIFADVMNVEIKQVADPIYANARGAAWIAAAGLGEIKF
                     ADVPELIQFRRAYQPQIKNRAVYDRGFENFQLIYKQIKGVYRRLNQ"
     gene            complement(453..1961)
                     /locus_tag="PLT08_15160"
     CDS             complement(453..1961)
                     /locus_tag="PLT08_15160"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HNE05863.1"
                     /translation="MSIRLRITFLYTFILALTLTVFGLALYTIQAQSTMDSLKHDLSV
                     SANRLADATLKTDFPNFNGNPPPNGQEPPNGQPPNGQEPPPGGQQPGDGNPPPTPIPF
                     DQFSNQQEFQSFSEREIARVLDVQGNLVSSPFGREGDALPLSAAGLQALQNGSDWWET
                     NIVSDESMLTYNRPVIVDNKVIYIVQVARSLTERNRTLQSLSTTLLLAGLLTTLIAFG
                     IGWLFSGLTLAPINRITKTAKEIGEERDFSRRVDYNGPQDEVGQLASTFNAMLTRLQD
                     AFKKVEHSLQMQRNFVADVSHELRTPLTTLRGNLGLLKRNPSPEIQNDILSDMVDESD
                     RLIRLVNDLLLLARADAGRSLSKEPVDVSIVLQEVCRQGNQLDANREINLDVPADTSI
                     IGDRDAFKQVALILLDNALKHSTGKINIRAEKYGKHIDVRVQDFGEGIPPETLAHVFD
                     RFYRGEDAATIPGFGLGLPIAKTLVEGFGGEIKIESEVGKGSTVIMRFPSAK"
     gene            complement(1961..>2187)
                     /locus_tag="PLT08_15165"
     CDS             complement(1961..>2187)
                     /locus_tag="PLT08_15165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HNE05864.1"
                     /translation="TVTEFNLLHMLLRHPRQVLERRQILNEVWGYDFGGDDNVLEIYI
                     GYLRKKLEVDGNSRLIQTVRGVGYVLREES"
BASE COUNT          540 a          487 c          573 g          587 t
ORIGIN      
        1 tggaagacaa aacgcttcgt gccggacttt ataacttgtc actcaactcc acacgtgagg
       61 atatcatccg cgcctttttg gaaggcattg cttataacac acgctggttg ctatcccctg
      121 tggaaaaatt cctgggtcgc aaagtgaata gcattaacat cgttggcggg ggagcgcagt
      181 ccgacctgtg gtgtcagatt tttgcggatg tgatgaacgt tgagatcaag caggttgcag
      241 accccatcta tgccaatgcg cgcggcgcgg cgtggattgc ggcggcaggc ttgggcgaaa
      301 ttaagtttgc cgatgttcca gagttgatcc aattccgaag ggcgtatcaa ccccaaatca
      361 agaatagagc cgtgtatgat cgtgggtttg aaaacttcca gttgatctat aaacagataa
      421 agggcgtgta tcgtcgtttg aatcagtaaa gtctacttgg cagatggaaa gcgcatgatg
      481 actgtgctgc ctttgccgac ttcgctttct attttgatct cgccgccaaa cccttcaaca
      541 agagtttttg cgattggcag acccagccca aaccccggaa tggttgcggc atcttccccg
      601 cgatagaaac ggtcgaagac atgtgcaagg gtctcgggtg gaatcccttc gccgaagtct
      661 tgaacgcgaa cgtcaatgtg ctttccatat ttttcagcgc gaatgttgat cttgcctgtg
      721 gagtgcttaa gtgcattatc tagcaggatc agtgcgacct gtttgaaggc atcacgatct
      781 ccaataatcg aagtgtcagc ggggacatct aggttaatct ctctatttgc gtcaagctga
      841 tttccctgcc tgcatacttc ttgaagaaca atagaaacat ccactggttc tttcgagaga
      901 ctacgcccgg catcggcgcg ggcaagcaag agcaggtcgt tgacgaggcg aataagacga
      961 tcgctttcat cgaccatatc gctaagaata tcattttgga tctctggcga aggattgcgt
     1021 ttgagcagcc caagatttcc gcgcaatgtg gtaagcggtg tgcgcaattc gtgtgagaca
     1081 tcggcaacaa agttacgttg catttgcaga gagtgctcta cttttttaaa tgcatcttgc
     1141 aagcgggtaa gcatggcatt gaaggtactc gcgagttgac caacttcatc ttgtggaccg
     1201 ttgtaatcga cccggcgaga aaaatctcgc tcttcaccga tctctttggc agttttggtg
     1261 atgcggttga tgggggctaa tgtaagacct gaaaaaagcc agccaattcc aaaagcaatc
     1321 agtgttgtaa gtaaacctgc aagcaataat gttgtagata aagattgcag tgtgcgattt
     1381 cgttcggtga gtgaacgagc tacctgaacg atataaataa ctttattatc tacaatgacg
     1441 gggcggttgt aggtaagcat gctttcatct gaaacaatgt ttgtttccca ccagtcgctg
     1501 ccgttttgta aggcttgcaa cccagcggcg cttaaaggca atgcgtcccc ttcgcgtcca
     1561 aaggggctgc tgaccagatt gccttgaaca tctaggactc tggcgatttc acgttctgag
     1621 aacgattgaa attcttgttg attggaaaat tgatcgaacg gtatgggggt aggaggtggg
     1681 tttccatctc cgggttgttg cccccctggt ggaggttctt gtccgttggg tggttgtcca
     1741 tttggaggtt cttgcccgtt cggaggaggg ttgccattga aattggggaa atccgttttc
     1801 agtgttgcat ctgctaaacg gttggcgctc acgcttaggt catgtttgag cgaatccatt
     1861 gtcgattgtg cttgaattgt gtatagcgcc agaccaaaga cggttaacgt caacgcgagg
     1921 atgaatgtat aaagaaaagt gatgcgaagc cgaatggaca ttatgattct tcacgcagaa
     1981 cgtagccaac cccgcggacg gtttgtatta ggcgcgaatt cccgtctact tcaagttttt
     2041 tacgcagata tccgatatag atctcaagaa cgttatcatc tccgccaaag tcgtagcccc
     2101 agacctcatt gagaatctga cgccgttcaa gcacctggcg tggatgccgc aatagcatat
     2161 gcaaaagatt gaattccgtc actgtga
//
LOCUS       DAOQJE010000680         2185 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_38913_length_2185_cov_4.991080, whole genome shotgun sequence.
ACCESSION   DAOQJE010000680 DAOQJE010000000
VERSION     DAOQJE010000680.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2185)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2185)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2185
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38913_length_2185_cov_4.991080"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            9..620
                     /locus_tag="PLT08_15170"
     CDS             9..620
                     /locus_tag="PLT08_15170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HNE05865.1"
                     /translation="MDLFLHLDEYLQTIITTYGAWTYGILFVVIFIETGLVVMPFLPG
                     DSLLFAAGTFAALGSFNVWGLVGLLAVAAVLGDAVNYSIGHYLGDRAYNIKWIKKEYL
                     EQTHAFFEKHGGKAIFLARFVPIVRTFAPFVAGIGKMSYSYFATYNIVGGISWVTIFT
                     LLGFFFGNIPFVKKNFELVIIAIILISVVPMIYEWWKARRAAK"
     gene            674..1333
                     /locus_tag="PLT08_15175"
     CDS             674..1333
                     /locus_tag="PLT08_15175"
                     /inference="COORDINATES: protein motif:HMM:NF017833.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exopolysaccharide biosynthesis protein"
                     /protein_id="HNE05866.1"
                     /translation="MNHIELSSKEESLLNQETLAEKMELVVEKLPTDEVTLVEIMDIV
                     GADSLMLVTIFMSLIFLVPISIPGVSTVFGTGILLIGITHLFARKLWLPKPIANRKLS
                     APKLKEAFKRAIVWLHRLEKVSRPHRLSKLTAEGGMTIFNNLSFILAALLLMAPFGFI
                     PFSNTLPALALIFFALGMMERDGGSVIFGHIANIATIIYFGFLIAGGGWSIGEFIKVL
                     G"
     gene            1379..1741
                     /locus_tag="PLT08_15180"
     CDS             1379..1741
                     /locus_tag="PLT08_15180"
                     /inference="COORDINATES: protein motif:HMM:NF037970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HNE05867.1"
                     /translation="MKYLAILFTLFILAIIILADKNALPPFIHAIYDFPNGDKAGHFI
                     LFGLLNFILTLTFLRALPNRTRSRVALSVGLILALAITLEEFSQRYFSSRTFDLIDLT
                     ASYIGVLVGGWIAFRIKK"
     gene            complement(1774..>2185)
                     /locus_tag="PLT08_15185"
     CDS             complement(1774..>2185)
                     /locus_tag="PLT08_15185"
                     /inference="COORDINATES: protein motif:HMM:NF013927.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0017000 - antibiotic biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="penicillin acylase family protein"
                     /protein_id="HNE05868.1"
                     /translation="LEKEYGQDPAKWPRWGELHTATFRNGTLGESGVFLIEDLFNRGP
                     FETNGGQSLVNATGWDLGTSFEVDWLPSMRMIVDFNDLNASLTVHTTGQSGHAYHPHY
                     DDMALFWADVSYYPMWWDQESVIDDAEGHLVLKP"
BASE COUNT          493 a          523 c          484 g          685 t
ORIGIN      
        1 aaagtgcgat cgatctcttt ttacatctcg atgaatattt gcaaaccatt atcacaacct
       61 atggtgcctg gacgtatggc attctttttg tagtgatctt tatcgaaacg ggcttggttg
      121 tgatgccctt cctgccagga gactccctgc tctttgctgc agggaccttt gctgcgctcg
      181 gctctttcaa tgtgtggggc ttggtcgggt tgttggcagt cgctgcggtg ttgggcgatg
      241 cggtcaatta ctccatcggt cattatcttg gtgaccgtgc ctacaacatc aaatggatta
      301 agaaagagta tttggaacaa actcatgcct ttttcgagaa gcatggcggc aaggctattt
      361 tccttgcgcg ctttgttcct atcgtccgta catttgctcc tttcgtagct ggtattggca
      421 aaatgtctta ctcttatttt gccacctata acattgtggg cggtattagc tgggtgacaa
      481 tctttacttt actcggcttt ttctttggca atatcccctt tgtgaaaaag aattttgagt
      541 tggtcattat tgccatcatt ttgatctcgg ttgtgccgat gatttacgaa tggtggaagg
      601 cacgtcgcgc cgcgaaataa actgtagtgt tctgaaatca ctttttactt tagaaaatac
      661 tgaggatatt aaattgaacc acattgaatt gtcttccaag gaagagtctt tgctaaatca
      721 agagacgctt gccgaaaaaa tggagttggt tgttgaaaaa ctccccacag atgaagtgac
      781 tttggtcgag atcatggata ttgtcggtgc tgatagcctc atgttagtaa ccatcttcat
      841 gtccttgatc tttcttgtgc cgatttccat tcctggcgtt agcacggttt tcggaacggg
      901 tattctcctg atcggtatta ctcatctttt tgcaagaaag ctttggctac ccaaacccat
      961 tgcgaatcgt aaactctcag cacccaaatt gaaagaggca ttcaaacgtg ctattgtttg
     1021 gttgcatcgt cttgaaaagg tcagccgtcc gcatcgcctg agcaaactca cggcagaagg
     1081 cggcatgacc attttcaata acttgagctt cattcttgcc gctcttctgc tcatggcgcc
     1141 ttttgggttc atcccgttta gcaacaccct tcctgccttg gcattgatct tctttgcgct
     1201 cggcatgatg gaacgagatg gcggttccgt gatttttggg catatcgcca atattgccac
     1261 gatcatctac ttcggcttcc tcattgcagg cggcggatgg tctattggcg aattcattaa
     1321 agtgcttggt taactcttta taaggcaggc tgggttccaa gcccagcctg ttttttttat
     1381 gaaatatcta gctattcttt tcacactgtt cattcttgcg atcatcattc ttgcagataa
     1441 aaatgcttta ccaccattta ttcatgcaat ctatgatttt ccaaatggcg acaaagccgg
     1501 gcacttcatc ctctttggac tcctcaactt tattctcact cttacctttc tccgcgcgct
     1561 tcccaaccgg acccgtagcc gggttgctct ttcagtcggt ttgatactcg ccctcgccat
     1621 caccctcgaa gaattctcgc aacgttactt ttcctctcgt accttcgatt tgattgatct
     1681 tacagcaagt tatataggtg ttttagtagg cgggtggatc gccttccgaa taaaaaagta
     1741 ggtcacgcct caagcgtgac ctactttttt attttagggc ttcaacacca aatgcccttc
     1801 cgcatcatca atgacggatt cttgatccca ccacattggg tagtaggaaa cgtctgccca
     1861 gaatagtgcc atatcatcgt aatgcggatg atatgcatgc ccagactgtc ctgtggtgtg
     1921 gaccgtaaga gacgcattga ggtcattaaa atccacgatc atacgcatgg agggcaacca
     1981 atctacttcg aaggatgtgc ccaagtccca gccagttgcg ttgactaggc tttgaccgcc
     2041 gttggtctcg aaaggtccgc gattgaagag gtcttcaatc aagaagacac cagattcgcc
     2101 aagtgtccca tttctaaacg tggcagtgtg taactcaccc cagcgcggcc atttggcggg
     2161 gtcttgcccg tattcttttt ccaat
//
LOCUS       DAOQJE010000681         2180 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_39082_length_2180_cov_5.215059, whole genome shotgun sequence.
ACCESSION   DAOQJE010000681 DAOQJE010000000
VERSION     DAOQJE010000681.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2180)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2180)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2180
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39082_length_2180_cov_5.215059"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..695)
                     /locus_tag="PLT08_15190"
     CDS             complement(<1..695)
                     /locus_tag="PLT08_15190"
                     /inference="COORDINATES: protein motif:HMM:NF017984.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0036297 - interstrand cross-link repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crosslink repair DNA glycosylase YcaQ family
                     protein"
                     /protein_id="HNE05869.1"
                     /translation="MLNIDIETARRFILGKQGLWPGRRWRGLKGTDHAMRTMEYLQLD
                     PLQIIARSQDISLYSRVLTYKPEMWEDAAYKQRNFFDWGGWLAARPMEELPYWRVVMH
                     RERDGGYGDSRLRQMGKEHAKAIVEVRAILKDRGTVGNRDFEAADRKKTQSYRGRKDS
                     ALALYYLWRIGDVMTHHRERFERVYALTENVAPQELIYEKTQEEADRFLVRKEISFGG
                     ITRIQRTGDSYGRG"
     gene            complement(700..1272)
                     /locus_tag="PLT08_15195"
     CDS             complement(700..1272)
                     /locus_tag="PLT08_15195"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014414.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNE05870.1"
                     /translation="MLNLIITSLLFLIVILSGLWMIVPAFYGPPSVPTRMNRIRKALQ
                     LANLQPGETVYDLGAGDGRVLLMAASEFHANAVGVEVGPVQCLLIKLRALSSGQGDKI
                     RVKWGSYFKVDLKDADVAFIYATSKETIKLAAHLKNQMKPGSRLVSISADFPEWEPTT
                     IDELELIFLYNMPPVAGNPTTYLMKKSNSS"
     gene            complement(1287..2159)
                     /locus_tag="PLT08_15200"
     CDS             complement(1287..2159)
                     /locus_tag="PLT08_15200"
                     /inference="COORDINATES: protein motif:HMM:NF014759.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-3' exonuclease H3TH domain-containing
                     protein"
                     /protein_id="HNE05871.1"
                     /translation="MKIHLVDGTYELFRAHFGAPPKKSKSGQEVGATLGMIRSMLLLL
                     SDPEVTHVAVAFDHVIESFRNKMYKDYKSSEGVDPVILNQFPVAEAAIKALGLVTWPM
                     VKYEADDAIATAVAKFKKVKSVEQIVICSVDKDLTQMVDGDRVICWDRRREILLNEKG
                     VVEKFGVSPESIPDFLALVGDSADGYPGIQGWGEKSTSAVLAKYKHIEAIPKDPNKIP
                     LGLGRATTLLENLQSNYKDALLFRELSTLRTDVPLKETLADLKWQGVYPRFKNVCEDL
                     GNQSIPERITKWRK"
BASE COUNT          574 a          593 c          448 g          565 t
ORIGIN      
        1 ccgcgtccat acgaatcgcc ggtgcgttga atgcgtgtga tgccgccaaa gctgatttct
       61 tttctaacga gaaagcgatc tgcttcctct tgagtctttt cgtagatcaa ttcctgtggg
      121 gcgacattct ccgtcagtgc atagacccgc tcaaatcgtt cgcgatgatg cgtcattaca
      181 tcaccgatgc gccacaaata ataaagcgct aatgcgctat ctttacgtcc acgataactt
      241 tgagtcttct tgcggtctgc tgcctcaaag tcgcgattgc ctaccgtccc tcgatctttc
      301 aaaatagcac ggacttcaac gattgcctta gcatgttctt ttcccatctg gcgcagacgt
      361 gagtcaccgt agcccccatc tcgttcacga tgcatcacta cccgccaata gggcaactct
      421 tccattgggc gcgccgccag ccagccgccc caatcgaaaa aatttctctg tttataggcg
      481 gcatcttccc acatttccgg tttgtaggtg aggacacggc tatataagga aatatcctga
      541 ctgcgtgcaa tgatctgcaa cgggtcaagc tgtaaatatt ccatggtacg catcgcatgg
      601 tcagtgcctt taagtccgcg ccaacgacga ccgggccaca agccttgttt tccaagaata
      661 aaacggcggg cagtttctat atctatattc aacataagtt taggaggaat tcgatttctt
      721 cattaaatat gtagttgggt ttcccgctac aggcggcatg ttatataaga aaatcaattc
      781 cagctcatcg atcgtagttg gttcccattc gggaaagtcc gcagaaatgg agacaagcct
      841 tgagccgggt ttcatttgat ttttcaagtg agccgcgagt tttatcgtct cttttgaagt
      901 ggcataaata aaagcaacat cagcatcttt caagtcaacc ttgaaataac tcccccactt
      961 cacacgaatc ttatcgccct gcccgctgct aagtgcacgc aacttaatca acaaacattg
     1021 aacgggtccc acttccacac ccacagcatt cgcatgaaat tcactggctg ccatcaacaa
     1081 aacacgtcca tcgccagcgc caagatcgta gacagtctct ccgggttgga ggttggcaag
     1141 ctgcaacgcc ttacggattc ggttcattcg cgtgggcacg gaaggcggac cataaaatgc
     1201 cggaacgatc atccacaacc cgctaaggat gacaattaaa aacagaagtg aagtgatgat
     1261 caggttaagc ataaccaaaa acgaatctac tttctccact tggtaattcg ttcaggaata
     1321 ctttgatttc ccaagtcttc acacacattc ttaaagcgcg gataaacacc ctgccatttc
     1381 aaatctgcca gcgtctcttt caacggtaca tccgttcgca aagtggataa ctctctaaac
     1441 aatagtgcgt ctttgtagtt actctgcaaa ttttcaagta aagttgtggc acgccccaag
     1501 ccaagcggaa ttttatttgg gtctttggga atcgcttcga tgtgcttgta cttcgccaac
     1561 accgccgaag tggacttttc accccagcct tgaataccag gatacccatc tgcagaatcc
     1621 cccaccaagg cgaggaagtc aggaatcgat tcaggactga caccgaactt ctcgaccaca
     1681 cctttttcat ttaacaaaat ctcacggcgt ctatcccagc aaataacacg gtcaccatcc
     1741 accatttggg tcaagtcttt atcgacagaa cagatgacaa tctgctcaac cgacttaact
     1801 ttcttgaact tcgctaccgc tgtagcaatc gcatcgtccg cttcatactt gaccatgggc
     1861 caggtcacca atcccaaggc tttgatcgcc gcctcagcca ccggaaactg attcaaaatg
     1921 accgggtcca cgccctcgct agatttatag tctttgtaca tcttgttgcg gaatgactca
     1981 atcacatggt cgaacgccac ggcaacatga gtcacttcag ggtcagaaag caacaagagc
     2041 atactgcgaa tcattccaag agtcgcgcca acttcctgtc cgcttttaga tttcttgggc
     2101 ggtgcgccaa aatgagcacg gaataattcg taggttccat ctaccaaatg aattttcatg
     2161 cagactcctt gtatgctctt
//
LOCUS       DAOQJE010000682         2175 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_39234_length_2175_cov_7.326887, whole genome shotgun sequence.
ACCESSION   DAOQJE010000682 DAOQJE010000000
VERSION     DAOQJE010000682.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2175)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2175)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2175
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39234_length_2175_cov_7.326887"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..660)
                     /locus_tag="PLT08_15205"
     CDS             complement(<1..660)
                     /locus_tag="PLT08_15205"
                     /inference="COORDINATES: protein motif:HMM:NF016206.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metallopeptidase"
                     /protein_id="HNE05872.1"
                     /translation="MFFLSPTYLMYMIPAFILMALTSWYVKSAYAKWSRVAAGSRFSG
                     YEATRRLIASSGLQGVKIQGTPGEMTDHYDPRDKTVYLSNGVANSASVAALAIAAHEL
                     GHAQQDAEEYFPLKFRGVLVPLVNIGSNLGWILIMIGLFLQLTQLAWLGILVFSGGAL
                     FALVTLPVEFDASARARRMLADSGIIQTDAEMRGVSNVLNAAALTYVAGLVTAVLQLL
                     YY"
     gene            complement(687..1955)
                     /locus_tag="PLT08_15210"
     CDS             complement(687..1955)
                     /locus_tag="PLT08_15210"
                     /inference="COORDINATES: protein motif:HMM:TIGR00225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HNE05873.1"
                     /translation="MNKTLKAVLLILAVAVVGLGSFAGGFITGHLLPFNNSSAFSFGE
                     APTAEPAPTASPEQQSSTPEDMQTLFAPFWEAWNLVHQNYVDQPVDDVALMRGAISGM
                     MQALGDQHSSYMDPEDFKQANQSLEGSYEGIGAYVDTTSDYLTITSPIPGSPAEEAGL
                     LPGDKIVAIDGEDMTGIDGELVRQKVLGPAGSTVVLTIMRDGEDKPLDFSIVRAQITI
                     ASATGKMLDGDIAYFQVTTFGSNTMPELEAALAEVMPQKPKAIILDLRNNGGGYLQTA
                     VEVASQFMGEGVVLYEQYGDGTRNTFEVIPGGKATDTSIPMIVLINEGSASASEIVAG
                     ALQDTGRAKLLGTVSYGKGSVQNWIPLSNDNGAVRITIAKWLTPNERTIHKIGLTPDY
                     PVDLTPEDRQADKDPQLDEAVRLLLEMLAK"
BASE COUNT          522 a          523 c          592 g          538 t
ORIGIN      
        1 gtaatacagc agctgtaaaa cggcggtgac caaacctgcc acataggtca atgcagcggc
       61 attgagaaca ttgctaacac cgcgcatttc ggcgtccgtt tggataatgc cgctatcggc
      121 taacatgcgt cgggcgcggg cagaggcgtc gaactccacg ggcaaggtga cgagagcaaa
      181 aagggcgccg cctgagaaaa ccaaaatccc gagccatgcg agctgtgtca attgcaggaa
      241 caagccgatc atgatgagga tccagccgag attggagccg atattgacaa gcggtactag
      301 cacgccgcgg aacttgagcg gaaaatattc ttctgcatct tgctgggcat gaccaagctc
      361 atgtgcggca attgccaaag ccgctacaga agcactgttc gcgacgccat tcgaaagata
      421 cacggtctta tcgcgcgggt cgtagtggtc ggtcatttcg ccgggagtgc cttggatttt
      481 cacgccttgc aaaccgctgg aggcgattag gcgacgtgtg gcttcgtacc cgctgaagcg
      541 gctgcctgct gcgactcggc tccattttgc atatgctgat ttgacatacc atgatgtaag
      601 cgccatcaaa atgaaggcgg ggatcatgta cattaagtaa gtgggactca agaaaaacat
      661 tcagttctcc tttctagttg attaagctat ttggcgagca tctccaagag gaggcgtaca
      721 gcttcatcca attgtgggtc tttatctgcc tgacggtctt cgggtgtcag gtctacaggg
      781 taatctgggg taagcccgat tttatggatg gtgcgttcgt tcggagtgag ccattttgcg
      841 atggtaatgc ggacggctcc attgtcattt gaaagcggaa tccagttttg tacggagcct
      901 tttccatagg agacggttcc aagcaacttg gcgcgaccgg tatcttgcaa tgcaccagct
      961 acgatctcag aagctgaagc agacccttcg ttgatcaata cgatcatggg gatgctggta
     1021 tcggtcgctt tgccgccagg gatcacttca aaggtgttgc gagtgccatc cccatattgt
     1081 tcgtagagca caacgccttc gcccataaat tgcgaggcaa cttccacggc ggtttgtaaa
     1141 taaccaccac cattgttacg caggtcgaga atgatagctt tcggtttctg cggcataact
     1201 tcagcgaggg cagcttccaa ctcgggcatg gtgtttgagc cgaaggtcgt cacttggaag
     1261 taagcgatat caccatcgag cattttgcca gtggcagagg caatggtgat ctgggcgcgg
     1321 acgatggaga agtctaatgg tttgtcttcg ccatcacgca taatcgtcag caccacggtg
     1381 gaaccggcag gtccgagcac tttctggcgg acgagttccc cgtcgatacc ggtcatatct
     1441 tcgccatcaa tggcaacgat cttatcgccg ggtaataaac ctgcttcttc agccggagag
     1501 ccgggaatgg ggctggtgat ggtcaaataa tcactggttg tatccacata ggcgccaatg
     1561 ccttcatagg aaccttccaa gctttgattg gcttgcttga aatcttcagg gtccatgtag
     1621 gacgaatgtt ggtcgcccaa tgcctgcatc atgccgctga tggcaccgcg catgagagca
     1681 acatcgtcta cgggttggtc aacataattt tggtggacga ggttccacgc ttcccagaag
     1741 ggagcgaaga gagtttgcat atcttcaggg gttgaagact gttgttctgg cgatgccgtg
     1801 ggggcaggct ctgcggtggg agcttcgcca aagctgaagg ctgaactgtt gttaaaaggc
     1861 aaaaggtgcc cggtaataaa gccaccggca aaagacccaa gcccaacgac ggcgacagcc
     1921 aaaataagca gtactgcttt taaagtttta ttcacaagat cctccaatag ctagcatacg
     1981 tagaaagata ccaattggtt attaaagatt gctgaatgaa ttattcgatt tgcgtaagaa
     2041 tcgaacggtt aaccttttac tcagaatccc cgcctgtttt gcccttgttc aattcatcga
     2101 ctgtgcggcg taattcttcc atttcgttct cttttttctg ctgggcgggg ttcatggctt
     2161 tcatcatttt gagca
//
LOCUS       DAOQJE010000683         2171 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_39356_length_2171_cov_6.166352, whole genome shotgun sequence.
ACCESSION   DAOQJE010000683 DAOQJE010000000
VERSION     DAOQJE010000683.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2171)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2171)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2171
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39356_length_2171_cov_6.166352"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1016
                     /locus_tag="PLT08_15215"
     CDS             <1..1016
                     /locus_tag="PLT08_15215"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit D"
                     /protein_id="HNE05874.1"
                     /translation="KTYQKSEVMTDRLDYMNPMGNNLSYVMAVEKLVDLDVPPRAQAL
                     RVILTELQRISSHLVLIGTWGLDLAAMSMFLYCFREREQILDIFELVSGQRMMTTFFR
                     PGGLWRDVPVEFEKAVRDFIKIFPKRIDEYEKLLTKNPLFVDRMVDIGYLSKETALSY
                     GVTGPNLRASGVNWDLRKARPYMGYEQYDFDVPVLTEGDTYARYLVRIEEFRQSLRII
                     EQALDKLPMGPVRSENRKFVPPPRSEIGVSMEALIHHFKLWTEGFHAPDASIYSAVES
                     PRGELGVLLAGDGGPKPRRVHMRTPSFDNLSVIPEIVKGHLVADLVAILASIDIVLGD
                     IDR"
     gene            1013..1507
                     /locus_tag="PLT08_15220"
     CDS             1013..1507
                     /locus_tag="PLT08_15220"
                     /inference="COORDINATES: protein motif:HMM:NF013426.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase subunit E"
                     /protein_id="HNE05875.1"
                     /translation="MSLEKKYSKEVKQILAKYPPEHKRSAVMPLLYLAQREEGYVNKE
                     SMKDIASILDITETEVASIIGFYSLYHDKKAGKYRMQVCNDLPCALRGSDEFLNKLCE
                     NLGVKVGETTEDGLITIEAVTCLASCDRAPMFQTQGPDGIKYHDYMTIDRTMELIEAL
                     KGGK"
     gene            1510..>2171
                     /locus_tag="PLT08_15225"
     CDS             1510..>2171
                     /locus_tag="PLT08_15225"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559260.1"
                     /note="part of NADH-ubiquinone oxidoreductase complex I;
                     shuttles electrons from NADH, via FMN and iron-sulfur
                     (Fe-S) centers, to quinones in the respiratory chain; NuoF
                     is part of the soluble NADH dehydrogenase fragment, which
                     represents the electron input part of NADH dehydrogenase;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit F"
                     /protein_id="HNE05876.1"
                     /translation="MAYVLLRHRDIPDIEKMSVYKKNGGFSALKNAITKMQPNDVIDV
                     VKNSGLRGRGGAGFPTGMKWSFIDKNSWPHYIVANADESEPGTFKDREIMEKNPFQFL
                     EGLALGAYAVGANAAYIYLRGEFWQVAAWLDEKIAEMEEAGYLGENLFGKDYSLKIYT
                     HLGAGAYICGEETALLESLEGKRGQPRVRPPFPPSVGLYGKPTIINNVETFTNVPMIL
                     ANG"
BASE COUNT          523 a          547 c          542 g          559 t
ORIGIN      
        1 ccaagaccta ccaaaaatcg gaagtgatga ccgaccgcct tgattatatg aaccccatgg
       61 gaaataacct ctcctatgtg atggctgtcg aaaagttggt agacctggat gtgccgccgc
      121 gtgctcaagc tttgcgcgtt atccttacag agcttcaacg tatttcctct cacctcgtgt
      181 tgatcggtac atggggcttg gatctggcag ccatgtccat gttcttgtac tgcttccgcg
      241 agcgtgaaca gatcctcgat atttttgaac tcgtctctgg tcaacgcatg atgaccacat
      301 tcttccgccc cggcggtttg tggcgcgatg tgcctgttga atttgaaaaa gccgtgcgcg
      361 atttcatcaa gattttcccc aagcgcattg atgaatatga aaaacttttg acgaagaatc
      421 ccctgttcgt ggatcgtatg gtggatatcg gttacctgag caaagaaacg gctctttcct
      481 acggggtaac gggtccgaac ctgcgagcct caggcgtcaa ctgggacctt cgcaaagccc
      541 gcccctatat gggctatgaa caatatgatt tcgatgtgcc ggttttgacc gaaggcgata
      601 cctacgctcg ttacttggtg cgtatcgaag aattccgtca gtctttgcgc attattgaac
      661 aggcgttgga taaattaccg atgggacccg tccgatcgga gaaccgcaag ttcgttcctc
      721 ctccccgttc agagatcggc gtaagcatgg aagcgctcat ccaccacttc aagttgtgga
      781 ccgaagggtt ccatgctcca gatgcttcta tttatagcgc agtggaatcc ccacgcggtg
      841 aactcggtgt gttgttggca ggcgatggcg gacccaagcc gcgccgcgtc cacatgcgca
      901 ctccatcatt cgacaacctt tccgtcatcc ctgaaattgt caagggacat ttggttgccg
      961 atctggttgc gatcctcgct tccattgaca tcgttctcgg agatattgac cgatgagtct
     1021 tgaaaagaaa tactcgaagg aagttaaaca aattcttgcc aaatacccgc cagagcacaa
     1081 acgctctgcg gtgatgccgc ttctttacct tgctcagcgt gaggaaggct acgtcaacaa
     1141 agagtcgatg aaagacatcg cctccattct tgatattacc gagacagaag tcgcttccat
     1201 tatcggcttc tattctttgt atcacgataa aaaagcaggg aaatatcgta tgcaagtttg
     1261 caacgattta ccttgcgcat tgcgcggctc ggatgagttt ctcaataaat tgtgcgagaa
     1321 cctcggcgtt aaagtggggg agaccactga agatggtctc atcaccattg aagccgttac
     1381 ctgtctcgcc tcttgcgacc gtgcccccat gttccaaact cagggaccgg atggcatcaa
     1441 atatcacgac tatatgacca ttgatcgtac tatggaattg atcgaagctt tgaagggagg
     1501 taagtaaata tggcttacgt ccttttacgc catcgcgata tccctgatat cgaaaaaatg
     1561 agtgtctata agaagaatgg tggtttctct gctctcaaga acgccatcac caagatgcag
     1621 ccgaatgatg tcatcgatgt ggtcaagaac tccggtctgc gcggacgtgg cggtgcgggc
     1681 ttcccgaccg gcatgaaatg gtccttcatt gataagaact cttggccgca ctacatcgtg
     1741 gcaaatgcag atgagtctga gccgggtacg ttcaaagacc gcgagatcat ggaaaagaat
     1801 cccttccagt ttttggaagg tctggcgctg ggtgcttatg cagtcggcgc aaatgctgcc
     1861 tatatttatc tgcgcggtga gttctggcag gttgctgcct ggctcgatga aaagatcgcg
     1921 gagatggaag aagccggtta tcttggtgaa aacctcttcg gcaaagatta ttctctaaaa
     1981 atttataccc acttgggcgc tggtgcttac atctgtggcg aagagactgc tttgctcgaa
     2041 tcccttgaag gcaaacgcgg acaaccccgt gtgcgcccgc cattcccgcc ttctgtgggt
     2101 ctgtatggca agccaaccat tatcaataac gttgagacct tcaccaacgt tccaatgatt
     2161 cttgccaacg g
//
LOCUS       DAOQJE010000684         2164 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_39627_length_2164_cov_3.794215, whole genome shotgun sequence.
ACCESSION   DAOQJE010000684 DAOQJE010000000
VERSION     DAOQJE010000684.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2164)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2164)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2164
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39627_length_2164_cov_3.794215"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..719
                     /locus_tag="PLT08_15230"
     CDS             <1..719
                     /locus_tag="PLT08_15230"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HNE05877.1"
                     /translation="LEKALHTKDEFMAAMSHELRTPLAGIMGMAEMLQSTAADSLNSK
                     QVGYLATIQQSSNRLLELINNLLEFTHLQSSTAALNLLPCSIAGVCQTALQQIIPVSN
                     SKQQQTHYTSMPSEIILQTDEKRLVKILLLLLDNASKFTPRGGELGIEVIGRSEAELV
                     EFTVWDTGIGISEENLTRLFKPFAQLDTSLARQYEGAGLGLALAKGLTDLLDGTISVQ
                     STLGKDSRFTVTLPWKYPSL"
     gene            complement(816..1202)
                     /locus_tag="PLT08_15235"
     CDS             complement(816..1202)
                     /locus_tag="PLT08_15235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE05878.1"
                     /translation="MDEKLLLVLKKKLSEYSGEDADLTNNTVYFAVNLRSRFVGLDGG
                     AITVEEKDGSLVVHYWLSYISPFLPFLVLDIFLFWLSSVVPSYEEASIFAKLIAGYLG
                     FCVLSIIANVSSFSSIIYEAWHSVKK"
     gene            complement(1306..1947)
                     /locus_tag="PLT08_15240"
     CDS             complement(1306..1947)
                     /locus_tag="PLT08_15240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE05879.1"
                     /translation="MKYLPYEDFEIQTSLSPDEIFHRFRAAVDTKRKWWIFTNKPFWG
                     EVNRHYLRIWPVGSFFQANDTIVFGTIVGNDVGCQVRVRMRFYFRSLIFWFLVLGMFW
                     SSCFGTIASLILAKIQTGLWDIGSSIWFLPSMGIFVFGYLFVMISFKSSTTSIKMHLE
                     EITETKMKNFHHFDRLFGLTEFKIVSSLFLGTLLISGVWILYQLTIIGTNVPF"
BASE COUNT          706 a          508 c          402 g          548 t
ORIGIN      
        1 cccttgaaaa agccctgcac accaaggatg aattcatggc agccatgagt cacgaactgc
       61 gcacccccct tgccggtatc atggggatgg cagaaatgct gcaaagtacc gccgcagatt
      121 cgctcaactc aaagcaggtt gggtatcttg caaccatcca gcagagcagc aacagattac
      181 tggagttgat caataatttg cttgagttta cccatttaca aagcagcaca gcagcactaa
      241 atcttcttcc atgctccata gccggggtat gtcagaccgc attacaacaa atcattccgg
      301 tatcgaattc aaagcaacag caaacccact acaccagtat gccttcagag ataatacttc
      361 aaactgatga aaaacgactg gtcaagatct tattattgct acttgataac gccagtaaat
      421 tcacccccag aggcggcgag cttggtattg aggtcatagg cagatcagag gcagagttgg
      481 tggagttcac ggtttgggat acaggcatcg gtatctcaga agaaaatctg actcgcttat
      541 tcaagccctt tgcacagttg gacactagtc ttgcccgtca atatgaaggg gctgggttag
      601 gtctggcgct tgcaaaaggt ttgacagacc tgctcgatgg aaccatctct gtccaaagca
      661 cattgggaaa agacagccgt tttaccgtca ccctgccatg gaagtaccca tctctttaga
      721 agtatatctg tccactctag caacttagaa ttagattgag cgtatttcat gtcgagagag
      781 ggcgagaaga aaactagaaa acaaacctga attacctatt ttttgacgga gtgccatgct
      841 tcataaataa tacttgaaaa cgatgacaca ttagcaatga tgctcaacac acaaaaccca
      901 agatatcctg caatcaattt tgcaaaaata gatgcttctt cgtaagatgg caccacagaa
      961 gacaaccaaa acaagaaaat atccagaact aaaaagggaa ggaacggtga aatatatgaa
     1021 agccaataat gaaccactaa actgccatct ttttcctcca cagtgatagc accaccatcc
     1081 agtccaacaa acctgctgcg aagattgact gcgaaataaa cagtgttatt ggtcaagtca
     1141 gcatcctcgc cgctgtattc tgaaagtttc tttttaagta ctagaagtaa tttttcatcc
     1201 ataccctcac tttttgttgg aagttctaat ctaaccgctg aactataagc aaaaaatttc
     1261 ctcattttcc ctcacaatag tgaaaaccca ctttgaggta gtacactaaa aaggcacatt
     1321 agtaccaatg atagttaatt gatacagaat ccaaacgcca gaaataagaa gcgttccaag
     1381 aaacagggag ctaacaatct taaattctgt aagtccaaag agccgatcaa agtggtgaaa
     1441 gttcttcatt ttcgtttcag ttatttcctc taagtgcatt ttgatgctag ttgtactact
     1501 tttgaaacta atcattacaa acaggtatcc aaaaacaaaa attcccatgc ttggtaaaaa
     1561 ccagatggaa gatccaatat cccacaatcc agtttggatt tttgctaaaa tcaaagaagc
     1621 tatcgttcca aaacaggaac tccaaaacat tccaagtacc agaaaccaaa aaataagact
     1681 acgaaaatag aaacgcatcc gcacccttac ctgacaccca acatcatttc caacaatcgt
     1741 tccaaatact attgtatcgt ttgcttgaaa aaagctaccc accggccaaa tccgaagata
     1801 gtgtcgattt acttcacccc aaaatggttt attcgtaaaa atccaccact ttctttttgt
     1861 atctacggca gcccgaaatc gatgaaatat ttcatcaggg gataaactag tctgtatctc
     1921 gaaatcttca tagggcaggt acttcatact caaattccca tcttatattg agtatacgcc
     1981 aaaacaattc tttgagttgc atcttactcc ccttggtatg tcgaattcca ctcgggcgag
     2041 tcggctggaa gacctgacta ttaaatttcc aaatccccga tccaaactgc ccaatcaacc
     2101 gccttcgcaa gcggagcgtc cgcggtgatg agtggcagat ccaatttttg agcgaggaca
     2161 gcgt
//
LOCUS       DAOQJE010000685         2164 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_39660_length_2164_cov_2.058321, whole genome shotgun sequence.
ACCESSION   DAOQJE010000685 DAOQJE010000000
VERSION     DAOQJE010000685.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2164)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2164)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2164
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39660_length_2164_cov_2.058321"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1165
                     /locus_tag="PLT08_15245"
     CDS             <1..1165
                     /locus_tag="PLT08_15245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE05880.1"
                     /translation="MKIPIYYRALWGSAFQIIPDHAFINSPTNVGFNTAAFLSTQTGW
                     LSTYRAYVGGEWKTGAALVDYAAINYSISPKLLLAILEYQGGALTQPAPPVSKNLLGL
                     RRPFWDSPYLQLVLAANTLNNGYYGWRTGTLLEFEDDNAILIRPDPWQNPGSVALQYY
                     YSRMFTGDEYNRAIGPDGLYKTYFTFFGDPWKNQFNLIPGSLAQPDLLLPFPQDQTWN
                     FTGGPHTGWGTGAPFAALDFAPPAEQSGCVSAKPENFATAVADGLVVRSSIDGLALDL
                     DRDGDERTGWVVYYLHLATDQRATLGSDLKAGDMIGYPSCEGGRSTGTHVHIARKYNG
                     EWIPADSSLPFVVSGWVPHNGLEAYEGTLTKGGLTVTACVCGDAYTSISANYP"
     gene            complement(1180..1650)
                     /locus_tag="PLT08_15250"
     CDS             complement(1180..1650)
                     /locus_tag="PLT08_15250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HNE05881.1"
                     /translation="MDVNLNWLGRMAFEGVGPSGFVQRLDSDSAVGGDNSGARPMEMI
                     AIGLAGCTAMDVISILAKKQQKVSDFHIKLHAERADDHPKVFTDSVIEYQVYGTDVDE
                     GALLRAIGLSAEKYCPAQAMLSKAFPIRLTYKIFDAETKSLVKEGEYQPKQKVA"
     gene            complement(1659..>2164)
                     /locus_tag="PLT08_15255"
     CDS             complement(1659..>2164)
                     /locus_tag="PLT08_15255"
                     /inference="COORDINATES: protein motif:HMM:NF020123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HNE05882.1"
                     /translation="LALGISFLLLKNETVSAGSSIDFSTVPAKVDFPAPDLSFIDLGT
                     DPVSLSDYRGSVVLVNLWATWCPPCREEMPTLQAFYEKYKSEGFVLLAIDQGETLPQV
                     LPYVKELKLTFPVWLDYSSEAGQAFETMNLPSSYVIDRNGQVRLMWIGGISKKNLEKY
                     VPDVIKE"
BASE COUNT          574 a          557 c          461 g          572 t
ORIGIN      
        1 aatgaagatc ccaatctatt atcgtgcgct ttggggcagc gcatttcaga tcattccaga
       61 tcatgccttt ataaacagtc cgacaaacgt tggcttcaat acagcagcat ttctctcaac
      121 tcaaacgggc tggctttcca cctatcgtgc ctatgtcggc ggcgagtgga aaactggcgc
      181 agcacttgta gactatgcag cgatcaacta tagcattagc cctaaattgt tattagcaat
      241 ccttgaatat caaggcggag ctctaaccca accggctcca ccggtcagca aaaacctact
      301 tgggcttagg cgtccctttt gggattcgcc atatctacaa cttgtgcttg ccgctaacac
      361 actcaacaac ggctattatg gttggcgtac tggcactcta cttgagtttg aagatgacaa
      421 cgctattctc attcgcccag acccgtggca aaaccccggc tcagtagcac ttcaatatta
      481 ttattcgcgc atgttcacag gagatgaata caaccgcgct attggtccag atggtcttta
      541 taaaacctat ttcacatttt ttggcgaccc ttggaaaaat caattcaacc ttatccccgg
      601 cagcttagca caacctgatc ttctcttacc atttccacaa gatcaaacct ggaattttac
      661 aggtggacct catactggct ggggtacagg cgctccattt gccgcactag actttgcgcc
      721 tcctgccgaa caatcgggct gtgtatctgc aaaacccgaa aactttgcca ccgctgttgc
      781 cgacgggttg gttgttagat ccagtatcga tggtttggca cttgatcttg atcgagacgg
      841 cgacgaacgt acaggttggg tggtttatta ccttcatctt gccacagatc aacgtgctac
      901 tctcggttca gacttaaagg ctggcgatat gatcggttat ccatcctgcg aaggcgggcg
      961 ctcaacaggg acacatgttc acatcgcaag aaaatataac ggcgaatgga ttccagcaga
     1021 tagctcatta ccctttgtgg tcagtggttg ggtgccgcac aacggtctag aagcttatga
     1081 gggcaccctt acaaaaggcg gattaaccgt cacagcctgt gtgtgcggcg atgcgtatac
     1141 ctccatcagt gcaaattacc cataaaacaa aaggtcgctt tacgcgacct tttgttttgg
     1201 ctgatactcg ccctctttca ctaaagactt tgtctctgcg tcaaatatct tatatgtgag
     1261 ccgaattgga aatgccttgg aaagcatagc ttgtgccgga caatactttt ctgccgatag
     1321 ccctattgcg cggagtaatg cgccttcatc cacatcggta ccatacactt gatactcgat
     1381 cactgagtct gtaaagactt taggatggtc atcagcccgt tctgcatgaa gttttatgtg
     1441 aaaatctgag accttttgtt gcttcttcgc aaggatagaa atcacatcca tggcggtaca
     1501 acctgccaag ccaatagcga tcatttccat cggtcgggcg ccactattgt caccacctac
     1561 agcagaatct gagtctaagc gttgcacaaa gcctgaaggt cctactccct caaatgccat
     1621 tcgtcccagc caattcaagt tcacatccat tatgtttttc actccttaat cacatctggc
     1681 acatatttct caagattctt ttttgagatc cccccgatcc acattaaacg aacctgcccg
     1741 ttccggtcaa taacatatga gctaggtaaa ttcatcgttt caaaagcttg tccagcttca
     1801 ctactatagt ccagccagac agggaaagtt agcttaagct ctttcacgta aggcaacacc
     1861 tgaggcagag tctcgccttg gtcaatagca agcaaaacaa aaccttctga tttgtatttt
     1921 tcataaaatg cttgcaaagt cggcatctct tcacggcagg gcgggcacca tgttgcccac
     1981 agattcacca agacaacact gccacgataa tctgaaaggg ataccgggtc agtgcccaga
     2041 tcgataaagc tcaggtctgg ggcaggaaaa tctaccttgg cagggaccgt cgaaaagtcg
     2101 atggatgaac cggcagagac agtctcattt tttaatagta aaaaagaaat tcctagggct
     2161 aaaa
//
LOCUS       DAOQJE010000686         2159 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_39798_length_2159_cov_7.401141, whole genome shotgun sequence.
ACCESSION   DAOQJE010000686 DAOQJE010000000
VERSION     DAOQJE010000686.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2159)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2159)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2159
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39798_length_2159_cov_7.401141"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1098
                     /locus_tag="PLT08_15260"
     CDS             <1..1098
                     /locus_tag="PLT08_15260"
                     /inference="COORDINATES: protein motif:HMM:NF012242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp70 family protein"
                     /protein_id="HNE05883.1"
                     /translation="EKAKIELSTVMETEINLPYITADASGPKHLQLKLSRSKFEQMTE
                     DLLQRCRKPFEAALKDAGMDASKLDEVVLVGGSSRMPMVQDLVRKLTNGKDPNKGVNP
                     DEVVAIGAAIQGGVLGGDVKDILLLDVTPLSLGVETMGNVMTVMIERNTTIPVRKTEV
                     YSTAADNQTAVDIHVLQGERPMAGDNMSLGRFRLDGLPPAPRGVPQVEVTFDIDANGI
                     LHVTAKDKATGKEQKVTITASTNLNKSDIERMVQESKQNEAADKKRREAIQVKNDADN
                     MTYQIEKALRDLGDKVPSAEKASIETKVNELKQAAQGDDIEHIKKLTEELQNTFHALS
                     QQMYAQGQGGQPQPEGGPAPDGNVVDGEVRE"
     gene            1147..>2159
                     /locus_tag="PLT08_15265"
     CDS             1147..>2159
                     /locus_tag="PLT08_15265"
                     /inference="COORDINATES: protein motif:HMM:NF024919.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C39 family peptidase"
                     /protein_id="HNE05884.1"
                     /translation="MYNLYMKKSFNRLWFIPIFIVLSIGVYYLPPVHSRLAWRIELVR
                     TQIKYWLNPPDEAVFQPSGETSPLTVETAVATTRAEFLLTLTPTSTPTPELVATIEPT
                     ITSTPLPTTVMLDGVKYEHQHGRTNYCGPANFSMALTYWGWDGNRDVIGKVVMPVNEK
                     DKNVMPYELQDFITNNVPGMTSIIRNGGDFETLKRLISAGYPVITEKGIYETDLNGKF
                     SWMGHYAFVTGYDDANQVIIYQDSYQPDPETNPGPNRRMGYEAFVEGWRAFNYIFVVV
                     FPVEKQENVLALLGPLADETQANRHALEVAKAESQTLYGIDQYFAWFNIGTSHVDLLE
                     YADA"
BASE COUNT          591 a          576 c          523 g          469 t
ORIGIN      
        1 gagaaagcca agatcgaact ctcgaccgta atggaaaccg agatcaacct gccgtacatc
       61 acagcagatg cttcgggtcc aaaacactta cagttgaagc tcagccgctc taaatttgag
      121 caaatgaccg aagacctgct gcagcgatgc cgtaaaccgt ttgaagcggc actcaaagat
      181 gccggcatgg atgctagcaa actcgacgaa gttgtcttag ttggtggttc ttctcgtatg
      241 ccaatggttc aggatctggt gcgcaagctc accaacggca aagacccgaa caaaggcgtg
      301 aaccccgatg aagtagttgc cattggtgca gctattcaag gtggcgtgct tggtggcgat
      361 gtcaaagaca ttctcttgct cgatgtgacc ccgctctctc ttggcgtgga aaccatgggc
      421 aacgtgatga ccgtcatgat cgaacgcaat accaccatcc ctgtccgcaa gaccgaggtg
      481 tactccaccg ctgcagataa tcagaccgcc gtagacattc atgtgttgca aggcgaacgc
      541 ccaatggctg gcgataacat gagcctggga cgcttccgcc tcgatggact tccaccagca
      601 ccgcgtggcg ttccgcaggt cgaagtgacc ttcgacatcg acgcaaacgg catcttgcat
      661 gtcaccgcca aagacaaagc cacaggcaag gaacagaaag tcaccatcac cgcttcgacc
      721 aatctcaaca agagcgacat cgagcgcatg gtgcaagagt ccaagcagaa cgaagctgcc
      781 gataagaaac gccgtgaagc gatccaagtc aagaacgatg cggacaatat gacgtatcag
      841 attgagaagg cattgcgtga tcttggcgat aaagttccat cggcggaaaa ggcttccatt
      901 gaaaccaagg tcaatgagtt gaagcaagcc gcacaaggcg atgacatcga gcacatcaag
      961 aagctgaccg aagaattgca gaacaccttc cacgcactca gccagcagat gtacgctcaa
     1021 ggtcagggtg ggcaacccca gcccgagggc ggtcccgcgc cggatggcaa cgtcgtcgac
     1081 ggtgaagtac gagagtaaaa aataatcaga actccgaagc ctcagaaact tcggagttct
     1141 ttttgtatgt ataatcttta catgaaaaaa agttttaacc gtctttggtt tattcccatt
     1201 ttcattgtcc tcagtattgg cgtttattat cttccgcctg ttcacagccg ccttgcatgg
     1261 cgtattgaac tcgtgcgcac gcaaatcaaa tattggctca acccgcccga tgaagctgtc
     1321 tttcaaccca gcggagaaac cagcccattg accgtcgaga cagcggttgc cacaacgcgt
     1381 gctgaatttt tattgacgtt gacgcccacc tccaccccga cgccagaact ggttgccacc
     1441 attgagccga ccattacatc cacaccttta cccactactg tcatgctcga tggcgtgaag
     1501 tatgaacacc aacacgggcg gacgaattat tgcggacctg caaacttctc tatggcgctt
     1561 acctattggg gttgggatgg caaccgcgat gtgatcggca aagttgtaat gcctgtcaat
     1621 gaaaaagata agaacgtgat gccctacgag ttgcaggatt tcatcacgaa caacgttccc
     1681 ggtatgacgt ctatcatccg caacggcggt gactttgaaa cgcttaaacg ccttatctcg
     1741 gcaggctacc cggtcattac cgaaaaaggc atctatgaaa ccgacctcaa cggcaaattc
     1801 agttggatgg gacattatgc ctttgtcacc ggttacgatg atgctaatca agtcatcatc
     1861 tatcaagata gttatcaacc cgacccagaa acaaaccccg gtccaaaccg cagaatggga
     1921 tacgaagcct ttgtagaagg ttggcgtgcc ttcaattata tttttgtggt ggtcttccct
     1981 gtggaaaaac aagagaatgt gctggcattg ctcggtccgt tagcagatga aacccaagcc
     2041 aatcgccatg cgcttgaggt cgccaaagct gaatcccaaa ccctttatgg cattgaccaa
     2101 tattttgcct ggttcaacat cggcaccagc catgtagact tgctcgaata cgccgatgc
//
LOCUS       DAOQJE010000687         2152 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_40018_length_2152_cov_7.285646, whole genome shotgun sequence.
ACCESSION   DAOQJE010000687 DAOQJE010000000
VERSION     DAOQJE010000687.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2152)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2152)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2152
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40018_length_2152_cov_7.285646"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            95..868
                     /locus_tag="PLT08_15270"
     CDS             95..868
                     /locus_tag="PLT08_15270"
                     /inference="COORDINATES: protein motif:HMM:NF012596.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HNE05885.1"
                     /translation="MTTTLLSTLKSGVLTLSLNRPERANAFNFEMTTALQSALTDAEK
                     DSQVRCVVLTGNGKVFSAGQDISEMKKGGGEISYAEHLEKTYNPLILQIREMGKPVVA
                     AVNGACAGAAFGIALACDLRIAHTNAYFVVGFSGIALAPDSGVSLLLPKLIGLGRAQE
                     YFYTNSPIPAQLAFNWGIVNRLGGFNYQRVVDQMVGDLAQGPREAFALGKKAFNEAIL
                     PNLASVLHGEGILQDVAGKSSEHQVAVKAFFEKRKPKYD"
     gene            1093..>2152
                     /locus_tag="PLT08_15275"
     CDS             1093..>2152
                     /locus_tag="PLT08_15275"
                     /EC_number="1.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Glu/Leu/Phe/Val dehydrogenase"
                     /protein_id="HNE05886.1"
                     /translation="MSGQINAFEMAQKQFDAVAKQLKLDSGVAETLRWPMREYAFRIP
                     VRMEDGSLKVFQGFRVQHNDARGPNKGGIRFHPAETLDTVRALATWMTWKCAVADIPL
                     GGGKGGIIVDPSTLTVREKEDLCRGWVRNMWKNIGPRNDVPAPDVGTTPQMMGWMMDE
                     YSKLVGHYTPGVFTGKPLGGGGSLGRTEATGYGVIYTVREAMKRLNIDPKKSVAVLQG
                     FGNVSQYAAIGFVEILGGTVACVSCYDRHDKKAYTYSKKGGIDPRFLLSIVDEYGTID
                     KKKALDSGYQVDDGDKWIEFEGNVLIPAALEGQVNGDTVKKVSKGVKIVAEGANGPTT
                     PEADEVFKKNGVFNIPDFL"
BASE COUNT          519 a          529 c          582 g          522 t
ORIGIN      
        1 gacgatttag acgattaccc cggcgaataa agttgattac aatcagtgac agtcatcaag
       61 aaggtgactg tcacttttgt ttataggatt agccatgacc actacgcttc tttccacgct
      121 caaatctggt gtgctcaccc tgagccttaa ccgccccgaa cgcgccaacg cctttaactt
      181 tgaaatgacc acagctttgc aaagcgcgtt gaccgatgcc gaaaaagatt cacaagtgcg
      241 ttgtgttgtc cttactggga atggcaaagt attcagtgct gggcaagata tttctgaaat
      301 gaaaaaaggc ggcggggaga tttcgtatgc agaacatttg gagaaaacct ataacccact
      361 cattttacaa attcgtgaaa tgggcaaacc tgttgtcgct gcggttaatg gagcgtgtgc
      421 aggcgctgcc tttggcatcg ctttagcctg tgacttgcgt attgcccaca ccaatgcata
      481 ttttgtggtt ggctttagcg gcattgctct tgcgcccgat tctggcgttt cattgttgtt
      541 accaaaactg atcgggttgg ggcgtgctca agaatatttc tataccaaca gccccattcc
      601 ggcacaattg gcctttaatt ggggcattgt gaatcggctt ggcggattca actatcagcg
      661 tgtggtagac caaatggtgg gcgacctggc tcaaggtccg cgtgaggcgt ttgcgttggg
      721 caagaaggcg ttcaatgagg caatccttcc taatctggca agcgtcttgc acggagaggg
      781 tatcctgcaa gatgtagcgg gaaagtcgtc tgaacatcag gtggcggtaa aagcgttctt
      841 tgagaagcgt aaaccgaaat atgactagcg tatcctattt gggggcaaca gggttattac
      901 atggcgtcag aaatcagcgt acttttgtca tattgacgca aaaatgaatc tcttgtatag
      961 ttttagagta gtattcgtgg aattaccgat cttatgaacc agcgttctta cataggctgt
     1021 gaataagcgg gcaccccgtc cggagtgccc gcttatattt tctcttaaac ctctacctag
     1081 gagagtaaaa aaatgtcagg acagatcaat gcgttcgaaa tggcacagaa gcaatttgat
     1141 gctgttgcca agcaattgaa gctcgactct ggcgtggctg aaaccctgcg ttggccgatg
     1201 cgcgaatatg cgttccgcat ccccgttcgc atggaagatg gctcgctcaa ggtcttccaa
     1261 ggcttccgtg tgcagcataa cgatgcgcgc ggacccaaca aaggtggtat tcgcttccac
     1321 ccggctgaga cgctcgatac tgtgcgcgcg ctcgccacat ggatgacctg gaaatgcgcc
     1381 gtggcagata ttccgctcgg cggcggtaaa ggtggcatta ttgttgaccc ctccaccctc
     1441 accgtgcgcg agaaggaaga cctttgccgt ggttgggtac gcaatatgtg gaagaatatc
     1501 ggtccacgca atgacgtgcc cgcccccgat gtaggtacca ctccgcaaat gatgggctgg
     1561 atgatggacg aatattccaa actcgtcggt cactacaccc ctggcgtctt cactggcaaa
     1621 ccccttggcg gcggcggctc gctcggccgt accgaagcca cgggttatgg tgtcatctac
     1681 accgtccgcg aagcgatgaa gcgccttaac atcgacccga agaaatctgt ggctgtgctt
     1741 cagggcttcg gaaatgtgtc gcagtatgcg gcaatcggtt tcgttgaaat tctcggtggt
     1801 accgtggctt gtgtttcttg ctacgaccgc catgacaaga aagcctacac ctatagcaag
     1861 aagggcggca ttgatccgcg cttcctgctc tccatcgtgg atgaatacgg caccatcgac
     1921 aagaagaagg cgctggactc gggctaccag gtggatgacg gcgataaatg gatcgagttc
     1981 gaagggaatg tcttaatccc tgccgcgctc gaaggacagg tcaacggtga tacggtcaag
     2041 aaggtctcca agggcgtcaa gatcgttgcc gaaggcgcga acggtcctac cactccggaa
     2101 gccgacgaag tgttcaagaa gaacggcgtg ttcaacattc cagacttcct ct
//
LOCUS       DAOQJE010000688         2148 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_40153_length_2148_cov_4.487817, whole genome shotgun sequence.
ACCESSION   DAOQJE010000688 DAOQJE010000000
VERSION     DAOQJE010000688.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2148)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2148)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2148
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40153_length_2148_cov_4.487817"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..957
                     /locus_tag="PLT08_15280"
     CDS             <1..957
                     /locus_tag="PLT08_15280"
                     /EC_number="4.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016846 - carbon-sulfur lyase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-cysteine desulfhydrase family protein"
                     /protein_id="HNE05887.1"
                     /translation="NLSKALGGPRIFVKRDDQTGLAFGGNKTRKLEFLIAEAREQGAD
                     LLISGGAMQSNHCRQTAAAAARYGFECKLVLTGVKPQQATANYLLDQLFGAEIITVED
                     RAFRDEILKKTFDDAVAAGKKPYLVPYGGSSPTGAMGYTFAMEELMQQINGDLGGFQN
                     LRGLEHGVDWIVFGTSSGGTHAGLVLGQRLFGYKGKVLGISIDEPEEELKSHVSDLAS
                     RASEKLGERINFSRNDVFATEDYCQAGYGVFGAGEREAIHLFAKHEGLLLDPVYTGRA
                     AAGMIDLIRKGFFKKDETILFWHTGGQPALFADKYSQDVIKT"
     gene            complement(1038..1283)
                     /locus_tag="PLT08_15285"
     CDS             complement(1038..1283)
                     /locus_tag="PLT08_15285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE05888.1"
                     /translation="MNFIDILGWVGSATYLIAFALNSAKRVTSDSFAYQGMNIFAGII
                     MTYYTFNHGAYSATALNSIWAIIGVVTLVNKRRAPQT"
     gene            complement(1283..2080)
                     /locus_tag="PLT08_15290"
     CDS             complement(1283..2080)
                     /locus_tag="PLT08_15290"
                     /inference="COORDINATES: protein motif:HMM:NF015438.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MOSC domain-containing protein"
                     /protein_id="HNE05889.1"
                     /translation="MITVSSLTYYPIKACRGFDVLASNIQRMGLEHDRRMMVVTPDGE
                     FLTQREYPRLALVTPTLKNEAVTLSAPNVESIQFTVQSTGTPSPVNIWSSSGVDSIDQ
                     GDEAAAWFSNWLGISVRLVHMADGFKRKVNPIYAVNADDHTSFADGYPILIISEESLQ
                     DLNSRLDSALPMNRFRPNLVVKGCEEFAEDTWKRIRIGNVEMALVKPCPRCVVTTIDK
                     ETLAKSKEPLKTLSGYRNHELGAIFGMNTIPLNEGKIETGMTVEILE"
BASE COUNT          514 a          537 c          570 g          527 t
ORIGIN      
        1 aatttatcca aagcccttgg cggaccgcgc atcttcgtca agcgcgatga tcagaccggg
       61 ttggcgtttg gcggtaacaa gacccgcaag ctggaatttc tcattgctga agcacgcgaa
      121 caaggcgcgg acttgctcat ctccggtggg gcgatgcagt ccaatcattg ccgccagaca
      181 gctgccgctg cggcgcgcta tggctttgaa tgcaagttgg tgttgactgg ggtcaagccg
      241 cagcaagcca ctgctaatta tttgcttgac caactcttcg gcgcggagat catcaccgtc
      301 gaagaccgtg ccttccgtga cgaaatacta aagaagacat ttgacgatgc agttgctgca
      361 gggaagaaac cctatctcgt tccgtatggc ggttccagcc caactggggc gatgggttat
      421 acctttgcca tggaagaatt gatgcagcag attaatggag acctcggagg ttttcaaaac
      481 ctccgaggtc tggaacatgg tgtggactgg atcgtctttg gcacatcctc tggcgggacg
      541 catgccgggt tagtgttggg tcagcgtttg tttggctaca aaggcaaggt gctgggaatc
      601 agcattgacg agcccgaaga agaactgaaa tcgcacgtat ccgacctcgc ttcacgtgcc
      661 agtgagaaac ttggcgagcg gatcaatttc tcgcgcaacg atgtgtttgc gacagaagat
      721 tattgtcagg caggctatgg cgtctttggc gcaggcgaac gcgaggcaat tcatcttttt
      781 gccaagcacg aaggtttatt gcttgaccct gtctacacgg gcagagccgc ggcagggatg
      841 attgacctta tccgcaaagg tttcttcaag aaggatgaga caatcctctt ctggcatacc
      901 ggtggtcaac ccgcgttgtt tgcggataag tattcgcagg atgtgatcaa gacctaataa
      961 gtattcacga aaatcgttaa atcaaaacac caccccgcgc aaggtaactt gtttggggtg
     1021 gtgttttatg agacctgtca ggtctgtggc gcgcgtcttt tatttaccaa cgtcaccacc
     1081 ccaatgatcg cccaaataga attcaacgcg gttgctgagt acgcgccgtg gttgaaggtg
     1141 tagtacgtca tgatgatgcc tgcgaaaatg ttcatgcctt gataagcaaa tgagtcgctg
     1201 gtcactcgct tggcagagtt caacgcaaaa gcgattaggt aagttgccga gccaacccag
     1261 ccgagaatgt cgataaaatt cattattcca aaatctccac ggtcatacca gtttcaatct
     1321 ttccttcgtt caggggaatc gtgttcatcc caaaaatcgc gccgagttcg tggttgcgat
     1381 agccgctcaa ggttttgagc ggttctttac ttttcgccag agtctcttta tcgatcgtcg
     1441 tcaccacaca gcgcggacat ggcttgacca gcgccatctc cacattcccg atgcggatgc
     1501 gcttccacgt atcttctgcg aattcttcac agcctttcac cacaaggttc gggcggaagc
     1561 gattcatcgg cagcgccgaa tcaagccgcg agtttaagtc ctgaagcgat tcttccgaga
     1621 tgatcagaat cgggtagccg tctgcaaagc tggtgtggtc atccgcgttc actgcataga
     1681 tcggattcac tttacgcttg aacccatctg ccatgtgaac aagccgcacc gagatgccca
     1741 gccaattaga gaaccatgcc gcggcttcat cgccctgatc gatggaatcc acaccgctgc
     1801 ttgaccagat attgacgggc gacggcgtgc cggtagattg aaccgtgaat tgaatggatt
     1861 caacattagg tgcggataaa gtgactgcct cattcttaag ggtgggcgtg accagcgcca
     1921 gacgcggata ttcacgctga gtcagaaact cgccatccgg cgtgacgacc atcatgcgcc
     1981 ggtcatgttc aagccccatg cgttgaatgt tgctcgcgag cacatcgaac ccgcgacagg
     2041 ctttgatggg atagtaagtg agtgaggaaa cagtaatcat agaatctctt tgtaaataag
     2101 ttttgaaatt tgagccatac actgatgggc ttcgtaatct ttattttc
//
LOCUS       DAOQJE010000689         2146 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_40216_length_2146_cov_6.643233, whole genome shotgun sequence.
ACCESSION   DAOQJE010000689 DAOQJE010000000
VERSION     DAOQJE010000689.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2146)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2146)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2146
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40216_length_2146_cov_6.643233"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            49..1137
                     /locus_tag="PLT08_15295"
     CDS             49..1137
                     /locus_tag="PLT08_15295"
                     /inference="COORDINATES: protein motif:HMM:NF013641.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE05890.1"
                     /translation="MDSFAFIIHPIDPKRDVSRKYPFLGKVLTEKQIDFFSTFFPPVY
                     ISEIEGITSKATGKQIKGWFLACPYTPRRMMQLPERTVYRKIIQTGRAAEELGANILG
                     LGAFTSVVGDAGVTIANSLDIPVTTGDSYTVMMAVQAIREAAKVMDIKIEDATVAVVG
                     ATGSIGRVCAELIAGEAARTLLIARDEKKLEDLRDRLKVQARSELCISTKMDVLKEAQ
                     LILTVTSAIHDVIQPEHLQPGSVVCDVARPRDVSAMVAAVRDDILVIDGGMVDVPGPV
                     NFHFNFGFPEGKAYACMAETIALALEGRFEDYTVGKDITVDRVKEITAIAEKHGFGMS
                     GFRSFEREVTEEQIEAVRRNARRGRAIK"
     gene            1207..2112
                     /locus_tag="PLT08_15300"
     CDS             1207..2112
                     /locus_tag="PLT08_15300"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNE05891.1"
                     /translation="MGTARLLVVEDDVDIANMLKIYFTGLQYDVDVANRGRDALEKTR
                     QVLPHLIVLDIMLPDIDGYEVCRTLRTNTRTSHIPVIFLTQKDERSDKLQGLELGADD
                     YITKPFDIEELKLRVQGAIRRAERESLTDPRSGLPAGRLIEEQLRRIIREKSWALLDL
                     RVNNFEAFKDVYGFVAGDDVMRFAAMLIGEVVDELGTPNDFIGHAGGDNFIIITTNEA
                     APLIRQKIKDRFAEEVQSHYNFIDRQQGFIQAPKAEGGVDKVGFMTFSAGLVSPELQS
                     FADIREITELAAEARRQDTATATGV"
BASE COUNT          536 a          480 c          605 g          525 t
ORIGIN      
        1 caaacaccct atcatcgcaa ccgcaaagca gaagttgagt catggtaaat ggatagtttc
       61 gccttcatca ttcaccccat cgatcccaag cgcgacgtta gccgaaaata cccgttttta
      121 gggaaggttc tcaccgagaa gcagatcgac tttttttcca cctttttccc cccggtttat
      181 atctctgaaa tcgagggaat tacctcaaag gcaaccggaa agcagattaa aggctggttc
      241 cttgcatgtc cgtatactcc aaggcgtatg atgcagttgc ctgagcgcac ggtctatcgt
      301 aaaattatcc agacaggtcg tgccgccgaa gaactcggtg ccaatatcct cgggcttggg
      361 gcgtttacgt cggtggtggg ggatgccggt gtgacgattg ccaacagtct cgatattcca
      421 gtcactactg gagactctta cacggtcatg atggcggttc aagccattcg tgaagccgcc
      481 aaggtgatgg atatcaagat cgaggatgcc acagttgctg tggtgggcgc cacgggttcg
      541 atcgggcggg tctgtgcgga gctgatagct ggtgaagccg cccgaacctt actcatcgcc
      601 cgggatgaga aaaagcttga ggatctgcga gacaggctca aggtccaggc aagaagcgag
      661 ctgtgcatca gcaccaagat ggatgtttta aaagaagcac agcttatcct cacggttacc
      721 agcgcgatcc atgatgtcat ccagccggag catttgcagc cggggagtgt ggtatgtgat
      781 gtagctcgcc cccgcgatgt ttctgcaatg gttgccgcgg tgagagatga tatattagtg
      841 attgacggtg gcatggtgga tgtgccaggt cccgtaaatt tccactttaa ctttgggttt
      901 cccgagggca aggcatatgc ctgtatggca gagacgatag ccctcgcgtt agaaggtcgt
      961 tttgaagatt acacggttgg taaagatatt acggtagacc gtgtgaagga gatcacagcg
     1021 atcgcagaga aacacgggtt tggaatgagc gggttccgct cgttcgaacg cgaagtgaca
     1081 gaggaacaga tcgaagccgt tcgccgcaat gcccgccgag gacgtgctat aaagtagaac
     1141 aataaaaaat gcaaggaaca atgctgtttg ctcaaatata agtttgacga tcagcaggag
     1201 gcttacatgg gaactgctcg ccttttggta gtggaagacg atgtcgacat tgccaacatg
     1261 ctaaaaatct atttcacggg tttgcaatat gatgtagatg ttgccaaccg tggacgtgat
     1321 gcgctggaga agactcggca ggttttgccg catctcatcg tattggacat tatgttgccc
     1381 gatattgatg ggtatgaggt ttgccgcaca ctgcgtacta atacgcgtac cagccatatc
     1441 cctgtcatct tcctgactca aaaagatgaa cgcagcgata agctgcaagg gttggaactg
     1501 ggcgcggatg actacatcac caagcctttc gatattgaag aattgaagtt acgcgttcaa
     1561 ggtgccatcc gccgtgcgga acgggagagc ctcaccgacc cgcgctcagg cttgccggct
     1621 gggcgtttga tcgaagagca gttacgccgc atcatccgtg agaagtcttg ggcattgttg
     1681 gatctgcggg ttaacaattt tgaagcgttc aaagatgtct atggtttcgt agcaggcgat
     1741 gatgtgatgc gctttgctgc gatgttaatc ggtgaagtgg tggatgagtt gggtacgccg
     1801 aatgatttta ttggtcatgc aggtggcgat aacttcatta ttatcactac caatgaggcg
     1861 gctcctttga tccgccagaa gattaaagac cgttttgcag aggaagttca gagtcactat
     1921 aacttcattg accgccagca gggctttatt caagctccga aggcagaagg cggagttgat
     1981 aaagtgggtt tcatgacatt ttcagcgggg cttgtctcgc ctgaactgca gtcctttgca
     2041 gatatccgag aaattacaga actggcggct gaagcgcgaa gacaagatac agcaacagcc
     2101 actggtgtct aaacgttagc gagtcttaaa tgtctattgg gttggg
//
LOCUS       DAOQJE010000690         2133 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_40669_length_2133_cov_5.327719, whole genome shotgun sequence.
ACCESSION   DAOQJE010000690 DAOQJE010000000
VERSION     DAOQJE010000690.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2133)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2133)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2133
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40669_length_2133_cov_5.327719"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..608)
                     /locus_tag="PLT08_15305"
     CDS             complement(<1..608)
                     /locus_tag="PLT08_15305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE05892.1"
                     /translation="MKKPLTLLTIALIINACAPVVNSTPNPSITPVIVTTSTNTISPT
                     SASALNSDVQKVFITDKYVIEIPKDFTVRENLSSSTTTVPIYRIETSTGAGFDIVLHP
                     YIVSSSPTPGKCIVSTDLDAGITSAPIFCEGLALTNSFTISENRMIKYESVISDLSLM
                     LCTANSPCPVEVPIEKRYSTTYVFVIADKSLDTVLEFYVGDA"
     gene            complement(845..2023)
                     /locus_tag="PLT08_15310"
     CDS             complement(845..2023)
                     /locus_tag="PLT08_15310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE05893.1"
                     /translation="MKNQFLYLFLFIFILGVSSCASANSETKTRDLLVYVIENYPEKR
                     MLFVYDPINNSQKQILAGWDIEEFSLSKNDRLAFSSLKDGHSSIYVLDYPFTENAPTE
                     IALDIPAENTPISWSPDGHYLLFDSVRANSKKLSVWDGKSVSDIYEYHEQVFGVAWSP
                     NGQVAFTDIYTFILPYDGDVSEIFVWNGKTTVSASQNPSGNDTHPAWSKDGQLAFLSE
                     RNEEYDIFIWDGTSKENGAPDVKTFINIAPEVTQYFSDPTWTNTDSIAFSGGSESDLH
                     AQIYEWDGQTAKNISQNPSFHNGGQTWRNDGYWSFITFFSSGQNLYVRDNINQTVLET
                     KGQYRPAWSHNGLLAFCVPEHPEWTLSIWNGKNIVEIAHGNFIAAKWNNGEDVFCSNG
                     "
BASE COUNT          641 a          407 c          446 g          639 t
ORIGIN      
        1 aaagcatctc cgacgtaaaa ctcaagtact gtgtcaaggg acttatcggc aataacgaat
       61 acataagttg ttgaatatct tttttcgatc ggcacctcaa cagggcaggg tgaatttgct
      121 gtacaaagca ttagtgataa atcactaatt acactttcat atttaatcat ccgattttcg
      181 gaaatagtga atgaattggt tagtgctagt ccttcgcaaa atattggagc agaggttata
      241 cctgcgtcaa gatctgttga aacaatgcat ttacctggtg taggtgatga cgaaacaatg
      301 taagggtgta atacaatatc aaatcctgcg ccagttgatg tctcaatcct atatatcgga
      361 actgttgtgg tggaacttga aagattttca cgaacagtaa agtctttggg aatttcaatt
      421 acatatttgt cggtaataaa tactttttga acatcactat ttaatgctga agcagaagtt
      481 ggtgagatag tgttagttga tgttgtgaca ataacgggag taattgatgg gtttggcgta
      541 gaatttacaa ctggggcaca agcattaatt attaaggcga ttgttagtaa ggttaatggc
      601 tttttcattg tattctcctt gcagaaaatg acgaatgaag gggatataaa gttccgtcag
      661 ggtatggtta attactaatt acattttgca aggaaacgcc caacggattg cgttacctgc
      721 gctggggcag ggacggcgaa gccgtccaac cagaaaaagg ctaaggcgta gaaaactgcc
      781 tgagattgcc gcagaatccc cagcgtcagg tgcacgcttt gttgggcgtt tttgaaatgg
      841 aacattaacc atttgaacag aaaacatctt ctccattatt ccactttgct gcaatgaaat
      901 tcccgtgagc aatctcgaca atatttttac cattccatat tgaaagtgtc cattcaggat
      961 gttcgggaac acaaaaggct aataacccgt tatgactcca agcaggacga tactgtcctt
     1021 ttgtttccaa tacagtttga ttgatattat ctcggacata taaattttgt ccacttgaaa
     1081 aaaaggttat aaacgaccaa taaccatcat ttctccaagt ttgtccaccg ttatgaaaag
     1141 atggattttg gctaatgttc tttgcggttt gaccgtccca ttcataaatc tgagcatgta
     1201 aatctgattc actaccaccg ctaaatgcaa ttgagtcagt gttagtccaa gttggatctg
     1261 agaaatactg agttacttca ggggcaatat taataaatgt cttgacgtct ggtgcgccat
     1321 tttcttttga tgtgccatcc caaataaaaa tatcgtattc ttcgtttcgc tctgaaagaa
     1381 atgctagttg cccgtcttta ctccatgcag gatgtgtgtc gtttccagat ggattttggc
     1441 tagcactcac cgtagtcttg ccgttccaaa caaaaatttc acttacatct ccgtcgtaag
     1501 gaagaataaa agtgtaaatg tcagtaaacg ccacttgccc atttggactc catgcgactc
     1561 caaatacttg ttcatggtac tcataaatat cagatacgct ctttccatcc cacacggaca
     1621 gttttttact atttgcccga accgaatcga ataaaaggta atgcccatca ggactccagg
     1681 aaattggagt gttttcagcg ggaatatcaa gagcaatttc tgttggggca ttttctgtaa
     1741 aaggataatc caaaacatag attgagctat gaccatcttt caatgatgaa aacgctagac
     1801 ggtcgttctt gctaagactg aattcttcaa tatcccagcc agccaaaatt tgcttttgtg
     1861 aattgtttat ggggtcataa acaaacaaca ttcgtttttc tggataattt tcaattacat
     1921 aaacaagaag gtctctcgtt ttcgtttcgc tattcgctga cgcgcaagaa cttacaccta
     1981 atataaagat gaagaggaat aagtaaagga attggttttt catgattttt gtttaacaag
     2041 ggaactgccc aacggcatgc gttacctgcg ctggggcggg gacggcgaag ccgtccagcc
     2101 agaaaaatga taaggcgtag aaaactgcct gag
//
LOCUS       DAOQJE010000691         2133 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_40693_length_2133_cov_2.206930, whole genome shotgun sequence.
ACCESSION   DAOQJE010000691 DAOQJE010000000
VERSION     DAOQJE010000691.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2133)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2133)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2133
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40693_length_2133_cov_2.206930"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1479
                     /locus_tag="PLT08_15315"
     CDS             <1..1479
                     /locus_tag="PLT08_15315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Eco57I restriction-modification methylase
                     domain-containing protein"
                     /protein_id="HNE05894.1"
                     /translation="ANLSNDEVFTPPNIANQMLDLLPAEIWRDKNATFLDPATKSGVF
                     LREIAKRLMVGLEKEIPDQQKRIDHIFTKQLFGIAITDMTALLARRSLYCSKKANGKY
                     SICEGFDTPDGNIKFGRVIHIWEGDHCKYCGANINEYDRDESLETHAYQFIHTDTPEE
                     LFNMKFDVIVGNPPYQLSDGGFGRSASPIYNKFVEQAKKLNPRYLTMIIPSRWFAGGK
                     GLDDFRKGMLNDDHIRKLVDFEDANEVFPGVDIAGGICYFLWERDSNGDCEVTNIQNG
                     NAVSSTRSLHEFKTFIRNGQAAPILRKILAKQENKMNEQATSRKPFGLPTTERPQKKG
                     DIILYWQKGEGPYSRKDITIGVEMIDKWKVITSRAGFDHAGNPGKDGKRRVLSKIDIL
                     PPGTICNETYIVIGCYETKKQAQNLVAYMQTKIFRFLVSLLMVSHDITKDTYSFVPIL
                     DMSVKWTDEALCKRYGITKAEFEFIESKIRPMGEDNGEEADE"
     gene            1545..>2133
                     /locus_tag="PLT08_15320"
     CDS             1545..>2133
                     /locus_tag="PLT08_15320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HNE05895.1"
                     /translation="MAKDFFPPRPESTPTIYAYEDTNPQFKGLLKIGYTAKSAQERVA
                     AQYPTLRPGAAPYRIVFEESAMKNDGTVFDDHEVHRYLRRSGVKNPNGEWFACSVKEV
                     QAAYIAVKTGELNEENRTLDFVMRPEQAEAVEKTAAYFLDFKKDKGNKGKTPHFLWNA
                     KMRFGKTFAAYQLAKKMGWKKVLVLTFKPAVQSAWE"
BASE COUNT          659 a          464 c          498 g          512 t
ORIGIN      
        1 gccaacctaa gcaacgatga agtttttacg ccgcccaaca ttgccaacca aatgttggac
       61 ttgttgcccg cagaaatttg gcgtgataaa aatgccacct ttcttgaccc cgctaccaag
      121 agcggagtct tcctgcgtga gattgccaag cgattgatgg ttggtttaga aaaagaaatt
      181 cccgaccagc aaaaacgcat tgaccacatt ttcaccaaac aattatttgg cattgccatt
      241 accgatatga ccgccctgct tgcgcggcgc agtttgtatt gctcgaaaaa agcaaacggc
      301 aaatattcaa tttgcgaagg ctttgacaca cccgatggca atattaagtt tgggcgcgtg
      361 attcacattt gggaaggcga ccattgtaaa tattgcgggg cgaatatcaa cgagtatgac
      421 agagatgaaa gcctcgaaac ccacgcctat cagtttattc acaccgatac tccagaggag
      481 ttgtttaata tgaaattcga tgtcatcgtt ggaaacccgc cttatcaatt gagcgatgga
      541 ggatttggta gaagcgcgtc gcctatttat aacaaatttg ttgagcaagc aaaaaaactt
      601 aatccccgat atttgacgat gattattcct tcacgttggt ttgctggtgg caaaggatta
      661 gatgattttc gcaaaggaat gttgaatgat gaccatataa gaaaacttgt tgacttcgaa
      721 gatgcaaatg aagtttttcc aggtgtagac attgcgggcg gtatttgtta cttcctatgg
      781 gaaagagatt caaatggcga ttgcgaagtt acaaatattc agaatggcaa cgcggtttca
      841 tcaactcgaa gtttgcatga attcaagacc tttattcgga atggacaggc agcccccatc
      901 ttgcggaaaa ttttagccaa acaagaaaac aagatgaatg agcaggctac aagcagaaag
      961 cctttcggct tgccaacaac agaaagacct caaaaaaaag gagatattat tctttactgg
     1021 caaaaaggtg aaggaccata tagccgaaaa gatataacta ttggtgttga aatgatagac
     1081 aagtggaaag tcattacttc tcgtgctggg tttgaccatg ctggcaatcc aggcaaagac
     1141 ggcaaaagaa gagttttgtc taaaattgat attcttccac caggaacaat atgcaatgag
     1201 acttatatag tgattggctg ttatgaaact aaaaagcaag cgcagaactt agttgcatat
     1261 atgcaaacaa agattttccg ttttcttgtt tccttgctga tggtatccca tgacatcacc
     1321 aaagatacct attcatttgt tccaattttg gatatgagcg tcaaatggac agacgaggct
     1381 ctttgcaagc ggtacggaat taccaaagca gaatttgagt ttatcgaatc aaagattcgt
     1441 cctatgggcg aggataacgg agaagaagcc gatgagtaaa gataactttg gcgggcagac
     1501 ttccgaagtc ttaaagactt cggaagtctt gaacggagag tccaatggct aaagacttct
     1561 tcccgccgcg ccctgaatcc acgcccacca tttacgccta cgaagacaca aacccacagt
     1621 ttaagggtct gctcaagatt gggtacaccg ccaaaagcgc gcaagagcgg gtggcggcgc
     1681 aatatcccac gttaagacct ggcgcggctc catatcgcat tgtgtttgaa gaatccgcca
     1741 tgaagaacga cggcacagtc ttcgacgacc acgaagtcca ccgttacctg cggcgcagcg
     1801 gcgtcaaaaa tcccaacggc gaatggttcg cgtgttcagt caaagaagtg caggcggcat
     1861 atatcgcggt aaaaacgggc gaactcaacg aagaaaaccg cacacttgat ttcgtcatgc
     1921 gccccgaaca agccgaagcc gtggaaaaga ccgccgccta ttttctcgat ttcaaaaaag
     1981 acaaaggcaa caaggggaaa actccgcatt tcttgtggaa cgccaaaatg cgtttcggca
     2041 agacctttgc cgcctatcaa ctggcaaaga aaatgggttg gaaaaaggtc ttggtgctca
     2101 ccttcaagcc tgctgtgcaa agcgcatggg aag
//
LOCUS       DAOQJE010000692         2132 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_40698_length_2132_cov_7.181993, whole genome shotgun sequence.
ACCESSION   DAOQJE010000692 DAOQJE010000000
VERSION     DAOQJE010000692.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2132)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2132)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2132
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40698_length_2132_cov_7.181993"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..404)
                     /locus_tag="PLT08_15325"
     CDS             complement(<1..404)
                     /locus_tag="PLT08_15325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE05896.1"
                     /translation="MMIKRFDPRIVLGILLILGGGLALAQTMGYLQNATDYFWGGVFI
                     LGGLAFLFLLLSDNWWAAFPGFILLALGTLIILPESLDDIGGAIFLGGIAISFWYVYF
                     TDRQGRWWAIIPGGVLTALSLLILASSYFEEYS"
     gene            complement(413..1198)
                     /locus_tag="PLT08_15330"
     CDS             complement(413..1198)
                     /locus_tag="PLT08_15330"
                     /inference="COORDINATES: protein motif:HMM:NF039187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5668 domain-containing protein"
                     /protein_id="HNE05897.1"
                     /translation="MNRNSLFWGTVLLLVGGLMLAGEMGIRLPNGNSLLSLFWPIFLI
                     GFGVWVLLSVFMRKSVEVEPASVDLQNARSANVRINHGAGEFRLHSGASGNQLMQGTF
                     AGGLSQASDLSGDVLNVKLQPSENHIMFPPFGWKEQFDWDMSFNASVPLALNLNLGAN
                     KADINLRDMTVTDIQLKSGASETVITVPSHGRLSLDCEIGAASLTVIVPDGVAIRARA
                     TMGAGDFKVDRTRFPSDESPDFASAPNAVDIHVKGGAASVRVK"
     gene            complement(1203..1730)
                     /locus_tag="PLT08_15335"
     CDS             complement(1203..1730)
                     /locus_tag="PLT08_15335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE05898.1"
                     /translation="MNKTGRSQLALGIILILIGGWFLLNQTMPEFRNFFEPYTQWPVN
                     MLLIGAGILVIGLVTGSPGLAVPAAIVAGIGGIFYYQETTGNYDSWSYMWALIPGFIG
                     VGTILQGLLGENTGHNLKRGLNLMVVSAVLFLVFAAFLGGWNILGEFGPAVLLILLGL
                     WVLGSGLYRSYRKKE"
     gene            complement(1823..>2132)
                     /locus_tag="PLT08_15340"
     CDS             complement(1823..>2132)
                     /locus_tag="PLT08_15340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE05899.1"
                     /translation="IIKPDGAIVIMDSPIYHDETSGQAMLDERKAQFLSRYGFASDTI
                     RSEGFLTYERMNSLGKLLGIKWQHIVPNYGWKWRFRPLMARLRAKREPAQFGLWLGKR
                     "
BASE COUNT          670 a          548 c          431 g          483 t
ORIGIN      
        1 gaatattctt caaaatagga agaagcaaga attaacaggg agagtgcggt taatacacca
       61 ccggggatga tcgcccacca ccgtccttgt cggtcggtaa agtagacata ccaaaatgaa
      121 atggcaattc cgcctaggaa gattgctccg ccaatatcat ctaatgattc aggaagaata
      181 atcagtgtcc ctaaagccaa gaggataaag ccagggaacg ctgcccacca gttatcgctc
      241 aatagtaaga aaagaaatgc caaaccacca agaataaaga ctccgcccca gaaatagtca
      301 gtggcgtttt gcaaatagcc catcgtttga gcaagggcaa gtccaccgcc caagatgagc
      361 aatatgccta aaacaatgcg tggatcaaaa cgttttatca tcatcaaatc tcctacttaa
      421 ccctaacaga tgcggctccg cctttgacgt ggatatctac tgcattggga gcagaagcaa
      481 agtctggcga ttcatcacta gggaagcgag tacggtccac tttgaaatcg ccggctccca
      541 tagttgctct tgcgcggata gcaacgccgt cagggacgat aacggtcaaa gacgcagcac
      601 caatttcaca gtccagagag aggcgcccat gagatggcac tgtgatcact gtttcacttg
      661 cgccagattt cagctgaata tctgtaactg tcatatcccg caagttaata tctgccttgt
      721 ttgcacccaa gtttaggttc aatgccaaag gtacagaagc attaaaggac atatcccaat
      781 caaattgctc tttccagcca aatggcggaa acatgatatg gttttctgaa ggttgcaact
      841 ttacattaag cacatcgccg cttaagtctg aggcttgact taacccgccg gcaaatgtac
      901 cttgcattag ttgatttcct gatgcgccgc tatgcaaacg gaattcaccc gcaccatgat
      961 taatacgcac attcgcagaa cgtgcatttt gcaggtcaac actggcaggt tcaacttcaa
     1021 cacttttgcg catgaacacg cttagcaaga cccaaacgcc aaacccaatg aggaaaatgg
     1081 gccagaataa actcaataaa gagtttccat ttgggaggcg aattcccatc tcaccggcga
     1141 gcatcaaccc gccaaccagc agcagaacgg ttccccaaaa caaagaattt cgattcattt
     1201 tattattcct tcttgcgata cgaacggtat aacccgctcc ccaaaaccca taagcccagc
     1261 aaaatcaaca acacagccgg accaaactcg ccaagaatat tccatccgcc aagaaaagca
     1321 gcaaagacaa ggaataaaac ggcgctgacc accataagat tcaagccacg cttcaggtta
     1381 tgacctgtat tctcgccaag caacccttga agaatggtgc ccacgccgat aaagccagga
     1441 attaacgccc acatataaga ccatgagtca taattgcctg ttgtttcttg gtaatagaaa
     1501 attccgccaa tgccagccac aatcgcagcc ggaacagcaa gaccgggtga gccagtgaca
     1561 agcccaatca ccaaaatacc cgcaccaatc aacaacatat tgacgggcca ttgggtatat
     1621 ggctcaaaga aattcctaaa ctcgggcatg gtttggttta gcaagaacca tccgccgatc
     1681 aggatcaaga taatgcccaa ggcaagttga gaacgaccag tcttattcat aaaaattctc
     1741 ctgtgatact cttgaaatta gtgtataccg tttcaagcaa ttttgaaagc ctcttcatat
     1801 acgcagaaac attaagtaag tttcagcgct tgcccagcca caaaccaaat tgagcaggtt
     1861 cccgtttcgc tcgtaagcga gccatcagcg ggcgaaacct ccatttccac ccgtaattgg
     1921 gaactatatg ctgccatttt atgccgagta gttttcctaa agaattcata cgttcatagg
     1981 tcaaaaagcc ttcactccga atagtatctg atgcaaaccc ataccgagac aaaaattgcg
     2041 ctttgcgttc gtctaacatg gcttgcccag aagtctcatc atgatagatt ggagaatcca
     2101 taatcactat cgctccatct ggcttaatga tt
//
LOCUS       DAOQJE010000693         2132 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_40703_length_2132_cov_3.891671, whole genome shotgun sequence.
ACCESSION   DAOQJE010000693 DAOQJE010000000
VERSION     DAOQJE010000693.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2132)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2132)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2132
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40703_length_2132_cov_3.891671"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(8..994)
                     /locus_tag="PLT08_15345"
     CDS             complement(8..994)
                     /locus_tag="PLT08_15345"
                     /inference="COORDINATES: protein motif:HMM:NF012415.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAP domain-containing protein"
                     /protein_id="HNE05900.1"
                     /translation="MKSTYPWIFATLLFLTLALKPSEKAGAQPEAFSPNRTAQEMIEK
                     VNTLRASNGLEPYVSDAILMKIAQSHADYIASTGVLTHFDKNGKRPYQRALDAGYAVG
                     GNLAAGGLLTEAIFSGSGVPDNAVIAAWQANEADSVALLSSSYEDVGVGISAANGTTY
                     YVLVAGSQNDSGIAVTPTFVSSVAPLGTGTNIPNTPKPDGQIYHVVRKNEALWSVAIL
                     YNTTIEELKLLNGLATDEIFEGQQLLVRAANTQTPTPSPVPVTATLGIPTSTATLPVT
                     PTKTSTPTPVPTPPTSLKSGGMVVGGITLVALLAAGLGAFLGKAKKNPNEGE"
     gene            1081..>2132
                     /locus_tag="PLT08_15350"
     CDS             1081..>2132
                     /locus_tag="PLT08_15350"
                     /inference="COORDINATES: protein motif:HMM:NF014296.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase C-terminal
                     domain-containing protein"
                     /protein_id="HNE05901.1"
                     /translation="MPGFTPGRRYQPPFLPFKRQPFKKRAFAAIERAVKGPLFGCRMC
                     GNCLLQETALICPMECPKGLRNGPCGGSTPERCYVDESRPCIWYKIYDRSFKLGREAL
                     LLEVLPPMDWNKAGTDAWSDVFEQAGKVGLKNIVTGLTSGEAGAVSQTMDSIFRPVRQ
                     PDWWQGDAEYHAPKYTEPTSELERRLKAGEFVVAAEVTPPLSINTEKLKQNIDMLKPY
                     VAAINFTDSASAIPRMSSWACSKMALDLGADPVMQITGRDHTRVNLQSSAMGASALGV
                     RNILCLTGDSMALGPGPQGRMDIVDLDSIQMLWVLRRLRDEKKYLDGREVKQAPQYFL
                     GAAASPFASNMKFQALR"
BASE COUNT          542 a          521 c          547 g          522 t
ORIGIN      
        1 cggcgtccta ttcgccttca ttggggttct ttttcgcttt tcctaaaaac gcacccaaac
       61 ccgcagctaa caaagcgacc agagtaattc cacccaccac catcccgccg cttttcaaac
      121 tcgttggtgg ggttgggact ggtgtgggcg tagaagtttt tgtcggcgtg acaggtaagg
      181 ttgctgtaga ggtaggaatt cccaacgttg ccgtaactgg aacaggtgaa ggcgttggcg
      241 tttgagtatt tgctgctcgc accaaaagtt gctgcccttc aaaaatctca tcggtagcca
      301 agccgtttaa taactttagc tcttcaatgg tggtgttgta taaaatggca acactccaca
      361 aggcttcatt tttacgcacc acatgataaa tctgcccatc aggcttgggt gtgtttggaa
      421 tattcgtccc agtccctagc ggggcgacag aactgacaaa ggttggcgtg acagcaatgc
      481 cagagtcatt ttgggagcct gccaccaaaa cataatatgt ggttccattg gcagcggaaa
      541 tgccaacccc gacatcttca taagaggatg aaagcaaagc gacagagtcc gcctcgtttg
      601 cttgccatgc ggcaatcaca gcattatctg gaacaccaga accggagaaa atagcttcgg
      661 ttaacaagcc gcctgcagca aggttcccac ccacagcata cccggcatcc aaagcacgtt
      721 gatatgggcg ttttccattt ttatcaaaat gggtcaatac acctgtgctt gcgatgtaat
      781 ctgcatgaga ttgggcaatc ttcatcaaga tcgcatccga tacataaggt tccagcccat
      841 ttgaagcacg caaggtattc accttctcga tcatctcttg ggcggttcgg ttgggagaaa
      901 aagcttcggg ctgggcaccc gccttttcgg acggtttcag ggcaagggtc aagaaaagaa
      961 gcgttgcaaa gatccagggg taggtcgatt tcattgggaa aaattatagc aggtgccttc
     1021 ccaaaagttg agagtcagag gtaaaattat atataatgta taatctttag gaggcagcat
     1081 atgccaggtt ttacgccggg tcgtcggtat caacccccat ttttgccgtt caagcgccag
     1141 cctttcaaaa aacgagcctt tgccgccatc gagcgcgcag tgaaaggtcc gctatttggg
     1201 tgccgcatgt gcggaaattg cctcctgcaa gaaactgcac taatctgccc catggaatgc
     1261 ccgaaagggt tgcgtaacgg accatgcggc ggctccaccc ccgaacgctg ctatgtggat
     1321 gaaagccgcc cttgtatttg gtacaaaatc tacgaccggt cgtttaaact tgggcgcgaa
     1381 gccttgttgc ttgaagtact gccccccatg gattggaata aagccggcac agatgcatgg
     1441 agcgacgtgt ttgagcaagc tggcaaagtg gggctaaaaa atattgtgac gggtcttact
     1501 tcgggggaag ctggtgcagt ttctcaaacc atggattcca tctttagacc ggtgcgccaa
     1561 cccgattggt ggcaaggtga tgcagaatac catgcaccca aatatacaga acccacctct
     1621 gaacttgaac gcagactcaa agctggcgaa ttcgtagttg ccgcagaagt aactccccca
     1681 ctttcgatca acactgaaaa gcttaaacaa aatattgata tgctcaagcc gtatgttgcg
     1741 gcgatcaact ttacagacag tgcttcggcg attccgcgca tgtcttcttg ggcatgtagc
     1801 aaaatggcac ttgatttggg tgcagaccct gtgatgcaaa ttactgggcg tgaccatacc
     1861 cgggttaact tgcaatcgag cgcgatgggg gcttcggcgt taggtgttcg caacatcctt
     1921 tgcttaaccg gcgacagcat ggcactgggt ccaggtccgc agggtcgtat ggatattgtg
     1981 gatttagaca gtatccagat gctttgggtc ttgcgccgtt tacgcgatga gaaaaagtac
     2041 ctagatgggc gcgaggtaaa gcaagcaccg caatatttcc taggtgcagc agcttcaccc
     2101 tttgcctcaa atatgaaatt ccaggctttg cg
//
LOCUS       DAOQJE010000694         2131 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_40727_length_2131_cov_6.484586, whole genome shotgun sequence.
ACCESSION   DAOQJE010000694 DAOQJE010000000
VERSION     DAOQJE010000694.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2131)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2131)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2131
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40727_length_2131_cov_6.484586"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            79..831
                     /locus_tag="PLT08_15355"
     CDS             79..831
                     /locus_tag="PLT08_15355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HNE05902.1"
                     /translation="MSGHSKWSTIKRKKGVADAKRGAIFTRLAREITIAAREGGDPDT
                     NFRLRLAVEKARAENMPKDNIERAIKKGTGEDKDAAVFEELTLEGYGPHGSAVMVSCV
                     TDNRVRTVSDVRHIFSKFGGNMAEAGAVGWQFDRKSYFSFPSSAMAFDKAFELGVEAG
                     ADDVVDSGEEIEIFGPVEAFKTIMDTLGKAKVQPAEAGLRLIAKQEIDLGVEEIMAVM
                     KVVENLEDLDDVQDVYHNVKVSDEALAALENA"
     gene            885..1367
                     /gene="ruvC"
                     /locus_tag="PLT08_15360"
     CDS             885..1367
                     /gene="ruvC"
                     /locus_tag="PLT08_15360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008821 - crossover junction
                     endodeoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HNE05903.1"
                     /translation="MTLALGIDPGTATTGYGFVRLMPDGELVAVDYGVILTPKEATAS
                     ARLVMLFDDLNKLLKKHKPETAAVEKLFFSKNVTTGIAVGQARGVILLALEKAGISTY
                     EYTPNEVKQAVAGYGSAEKRQVQEMVRALLQLEKIPKPDDAADALAIAITHLNTARYD
                     "
     gene            1425..2003
                     /gene="ruvA"
                     /locus_tag="PLT08_15365"
     CDS             1425..2003
                     /gene="ruvA"
                     /locus_tag="PLT08_15365"
                     /inference="COORDINATES: protein motif:HMM:NF001040.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009379 - Holliday junction helicase
                     complex [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HNE05904.1"
                     /translation="MIATLRGEVSHVEDNAIIVEVGGVGMRVFVPAPMRTKASAGEMI
                     FLFTHLVVREDALTLYGFESQSDRDLFNILLGVDGVGPKVALSVLSTLTLETIQRAIF
                     ADEEELLSKVPGVGKKTAQKMAIHLKDKLKPTDALSKMAALADYDSEVLAALAALGYS
                     VVEAQAALQALPKDAPKDVEERLRVALQYIGK"
BASE COUNT          555 a          574 c          536 g          466 t
ORIGIN      
        1 gaaaccagaa tggtataatt tcgccgctta attcatcatt ccccaaccta aattggtcat
       61 tatctgaagg agaacgacat gtccggacat tctaaatggt ccaccattaa acgcaaaaaa
      121 ggtgttgccg acgccaaacg cggcgccatc ttcacccgct tggcgcgtga aattaccatt
      181 gctgcacgcg aaggcggcga ccccgatacc aacttccgct tgcgcctggc tgtagaaaaa
      241 gcccgcgccg aaaatatgcc caaagacaac atcgaacgtg ccattaagaa aggcaccggc
      301 gaagacaaag atgccgccgt gttcgaagaa ctcaccctcg aaggctacgg tccgcatggc
      361 tcggctgtga tggtctcctg cgtaaccgac aaccgcgtac gcaccgtttc ggatgtacgt
      421 cacatcttca gcaagttcgg cggcaacatg gcagaagccg gagccgtggg ctggcaattt
      481 gaccgcaaat cctacttctc cttcccctcc agcgccatgg cgttcgataa agcctttgaa
      541 ctcggtgtgg aagccggagc cgacgatgtc gtggatagcg gcgaagagat cgaaatcttt
      601 ggtcccgtag aagccttcaa aaccatcatg gatacgctcg gcaaagccaa agtgcagcct
      661 gctgaagccg gtctgcgttt gatcgccaaa caagagatcg acctcggcgt agaagagatc
      721 atggctgtaa tgaaggtcgt cgaaaacctc gaagacctcg acgatgtgca agatgtctac
      781 cacaacgtca aagtctctga cgaagcactc gcagccctcg aaaacgcgta attcaatttt
      841 acgtaggggc gggtctcaga cccgccccta catcatttaa tgatatgact ctcgccctcg
      901 gaattgaccc cggcaccgcc accaccggct acggattcgt gcgcctcatg ccagacgggg
      961 aactggtcgc tgttgattat ggcgtgatcc tcaccccgaa agaagccacc gcctccgctc
     1021 gcctggtgat gctctttgac gatctcaata agcttctgaa gaaacataaa cccgaaactg
     1081 ctgccgtaga aaaattattt ttctcgaaga atgtcaccac cggcatcgcg gtcggacaag
     1141 cccgtggcgt gattttgctg gcacttgaga aagccggcat ttctacctac gaatacaccc
     1201 caaatgaagt caaacaagcg gtagcggggt acggctccgc tgagaagcga caagtgcaag
     1261 aaatggtgcg cgctttattg cagttagaga aaatccccaa acctgacgat gccgccgatg
     1321 cattagcaat tgccatcacg catttgaata cagctcgata tgattaatta tgtaggggcg
     1381 actcgcgagt cgcccctaca ttttatttac ggtaaaatac aaacatgatt gccaccttac
     1441 gcggagaagt atcacacgtt gaagataatg ccatcatcgt cgaagtaggc ggtgtgggca
     1501 tgcgagtctt cgtcccggcg ccgatgcgca caaaggcttc ggcgggagaa atgatctttt
     1561 tgttcaccca tctcgtcgtc cgggaagatg cgctgactct ttatggcttc gaatcccaat
     1621 cagaccgcga cctcttcaac atcctgctcg gtgttgatgg cgtgggtccc aaagtggcgc
     1681 tctcggtgtt atccacgctc acgttggaaa cgattcaacg tgccatcttt gccgacgaag
     1741 aagaattgct tagcaaagtt ccgggtgtgg gcaaaaagac cgcgcaaaaa atggcgatcc
     1801 atctcaaaga caaactcaag ccgactgatg cgctttctaa aatggcagct cttgccgact
     1861 acgatagcga agttctcgcc gcgcttgccg cattgggtta ctcggtggtc gaagcgcaag
     1921 ccgcattaca agcgcttccc aaggatgctc ccaaagatgt cgaagaacgt ttgcgcgtgg
     1981 cgttgcaata catcggcaaa taatggctct actatttgga taaggtctaa atcaaaagag
     2041 actgtcacga tgaaggtgac agtctcttta tttggcatgt tgctaaaacc tatcgttgaa
     2101 ctgggacggg tttcttgacg gggaataagc c
//
LOCUS       DAOQJE010000695         2128 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_40826_length_2128_cov_6.281717, whole genome shotgun sequence.
ACCESSION   DAOQJE010000695 DAOQJE010000000
VERSION     DAOQJE010000695.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2128)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2128)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2128
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40826_length_2128_cov_6.281717"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..180
                     /locus_tag="PLT08_15370"
     CDS             <1..180
                     /locus_tag="PLT08_15370"
                     /inference="COORDINATES: protein motif:HMM:NF019581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HNE05905.1"
                     /translation="YTRDAAYAEFMENEKGQIKEGYLADLVLFSHDLFALPKENIMET
                     KPVLTMVNGKVVYEA"
     gene            189..1163
                     /locus_tag="PLT08_15375"
     CDS             189..1163
                     /locus_tag="PLT08_15375"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNE05906.1"
                     /translation="MGTYALRRILQTIPILFIISILLFLMVRAAPGGPLTAARRNPNI
                     SKEQIEAIEEKLGLNDPLPVQYGRWLGDMLRGDMGDSIKFRRPVSEMIGERVPNTLLL
                     VGASFLVTLLFAIPIGILSARKPYSKFDYAMTTVTFIGQAIPVYWLGLGLIVIFYVTI
                     KNPFTGDPLFPVGGMNTRGMEGNLLDTLWHLVLPVTALSLGWVAWYSRFLRSSMLDVL
                     HEDYVRTAHAKGLTDSLVYYKHALQNAILPLVTLIALDLPSLFAGALFVETIFSWPGM
                     GRLFWDAAKGRDYPVLLGVVMLTAVLIILCNIIADLAYGWLNPQVKYE"
     gene            1156..2058
                     /locus_tag="PLT08_15380"
     CDS             1156..2058
                     /locus_tag="PLT08_15380"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNE05907.1"
                     /translation="MNESSPLIKEQSMATVITRRFFKHKLAGVAITVLALLVLFSALA
                     SLTPYEPTEQNPTNIFQKASGAHWFGTDELGRDVFTRILYGGRVSLAVGLLSTLLSIS
                     LGVVMGALSGYFGGLLDSTLMRITDAFLTFPTIFVLIILGAFLREQQLYILQNSIFVV
                     IIIIAALSWMWPARLVRGLFLVLREKEFVTASRAMGGSDLRIIVRHILPNCVGPILVS
                     GTLQMASAIITESGLSYLGFGVQPPTPTWGSILSTAQNQVFRAPWLAFYPGLMIFVTV
                     MAINYIGDGLRDAFDPYVIHTKSQ"
BASE COUNT          464 a          528 c          526 g          610 t
ORIGIN      
        1 tatacccgcg atgcggcgta tgccgagttt atggaaaatg aaaaaggtca aatcaaagaa
       61 gggtatcttg cagatttggt cttattctcg catgacctat ttgctttgcc caaagaaaac
      121 attatggaaa ccaagccggt cttgaccatg gtaaatggca aggtcgtata cgaagcctag
      181 aggtactcat gggaacttac gccctgcgtc gcattttaca aaccattccc attcttttca
      241 tcatcagtat tttattgttt ctgatggtgc gtgccgcgcc aggcggaccg ttaacagccg
      301 ctcgcaggaa cccaaatatt tcgaaggaac agattgaagc cattgaagaa aagctcggat
      361 taaatgatcc tttacctgtt caatatggac gttggctcgg cgatatgctg cgcggtgata
      421 tgggcgactc gatcaagttt cgccgcccgg tttcagagat gatcggtgaa cgcgtgccca
      481 atactctgtt attggtgggc gcctcttttc ttgtgacctt gttatttgcc atccccattg
      541 gcattctctc cgctcgaaaa ccctactcca aattcgatta cgccatgacg acggtcacct
      601 ttattgggca ggcaattcca gtgtattggc tgggtttggg actcattgtg atcttttatg
      661 tcaccatcaa aaatcctttc acgggggacc cgcttttccc ggttggtgga atgaacaccc
      721 gtggcatgga aggaaatttg ctggacacgc tctggcattt ggtcttgccc gttaccgccc
      781 tcagtttggg atgggtcgct tggtattcac ggtttctacg ctccagcatg ttagacgtgt
      841 tacacgaaga ttacgtccgc acggcacatg caaaagggtt aacagatagt cttgtctatt
      901 acaaacatgc tttgcaaaat gcgattctgc cactggtcac cttgatcgcg cttgacttgc
      961 ccagtctttt tgcgggtgcg ctatttgtag agaccatctt ctcctggccc ggtatggggc
     1021 gcctcttttg ggacgcggca aaagggcgag attaccccgt cctccttggg gtcgtgatgc
     1081 tcactgccgt tcttatcatc ctctgcaaca tcatcgcgga tctggcttat ggctggctca
     1141 acccacaggt gaaatatgaa tgagtcgagt ccgcttatca aagaacaaag catggcaact
     1201 gtcatcacgc gtcgcttctt caagcataaa ttggcgggtg ttgccattac ggtcttggca
     1261 ttgttggtgt tgttctcggc gttggcaagc ctaacgccct atgaacccac agaacagaac
     1321 ccgacgaata tttttcaaaa agcatccggt gctcattggt ttggcaccga cgaacttggt
     1381 cgggatgtgt tcactcgcat cttatatggc gggcgagtct cgttggcagt tggcttgcta
     1441 tccactttgt tatccatttc gttaggggtt gtcatgggtg cattaagtgg ctactttggc
     1501 ggcctgctcg actcgaccct gatgcgtatc acagatgcct tcctaacctt ccccaccatc
     1561 tttgtactca tcatccttgg cgcctttttg cgcgaacagc aattgtacat tttgcaaaac
     1621 agcatcttcg ttgtcatcat cattatcgcc gccctctcgt ggatgtggcc cgcccgcctc
     1681 gtgcgcggac ttttcctcgt gctgcgtgaa aaagaatttg tcaccgcctc gcgtgccatg
     1741 gggggcagcg atttgcgcat tattgttcgc catattttgc cgaattgtgt cggtccgatc
     1801 ttggtgagcg gaactttgca aatggcttcg gcaatcatca ccgagtctgg cttgagttat
     1861 ttagggtttg gtgtgcagcc cccaacgcct acatggggca gcattttatc cactgcacaa
     1921 aatcaagtct tccgtgcgcc gtggctggcg ttttacccag gcttgatgat cttcgtcacc
     1981 gtgatggcga tcaactatat tggcgacggg ttaagagatg cgttcgaccc gtatgtgatc
     2041 cataccaaat cgcaatagag tttataaatc aaaaaggaga agagatgaaa ctcaaactgt
     2101 ttacaatgct tgttattttg ggtctggt
//
LOCUS       DAOQJE010000696         2118 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_41179_length_2118_cov_7.692681, whole genome shotgun sequence.
ACCESSION   DAOQJE010000696 DAOQJE010000000
VERSION     DAOQJE010000696.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2118)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2118)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2118
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41179_length_2118_cov_7.692681"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..625)
                     /locus_tag="PLT08_15385"
     CDS             complement(<1..625)
                     /locus_tag="PLT08_15385"
                     /inference="COORDINATES: protein motif:HMM:NF013630.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HNE05908.1"
                     /translation="MTLRTPSTPPSGTIGSYRRRNRGRQNAIVIVAGVLLLGGLALLI
                     YWMTLPGKPINLLLATETPTPTLTYTPTNTNTPTPTFTETPSPTITFTPTFSAPFTYT
                     VQDGDSLAVIAERFNLGEDAIPLILLLNPYVVPTDPAQFPQGVDPTTLNVIPGQVILL
                     PNPDMQLPTATPIPPNLPRGTKIDYVIQSGDSLGAIANAFNSTIDDIM"
     gene            complement(769..>2118)
                     /gene="uvrA"
                     /locus_tag="PLT08_15390"
     CDS             complement(769..>2118)
                     /gene="uvrA"
                     /locus_tag="PLT08_15390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016099277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HNE05909.1"
                     /translation="QRIRLATQVGSRLVGVLYVLDEPSIGLHPRDNTRLLETLKGLRD
                     LGNTVLVVEHDDETILESDWVIDLGPAAGEHGGQIIAEGTPKQILAHPKSLTGLYLSG
                     RKQVEIPKKRREGNGKLLKIVNATANNLKGIDVNIPLGKLVCITGVSGSGKSTLMSDI
                     LYNELAAKLMGARTQSGEHKTIEGIEHLDKIINIDQSPIGRTPRSNPGTYTGLFDEIR
                     KLFAELPESKVRGYKPGRFSFNVHGGRCEACQGQGELRIEMQFLPDVYVPCDVCHGKR
                     FNRETLQVMFRDTYSIADVLDMTVDHALEEFKNYPQMQNKLKLLQEVGLGYVRVGQPA
                     TTLSGGEAQRVKLSKELSRRATGRTLYVLDEPSVGLHAADVHKLIEVMQRLVDTGNSV
                     LIIEHNLDIIKVADWLIDLGPEGGNRGGELIAEGTPEQVMKVKGSYTGKYLKDHINR"
BASE COUNT          527 a          501 c          525 g          565 t
ORIGIN      
        1 tcataatatc gtcaatagtg ctattgaagg cattggcaat ggcacctaaa gagtcaccag
       61 attggatgac gtaatctatt ttcgtgccgc gcggcaagtt aggtggaatg ggtgtggctg
      121 taggcaattg catatctggg tttggaagca aaatcacctg cccaggaatc acgtttaggg
      181 tagttgggtc aacgccttgt gggaattgag ccggatcggt tggaacaaca tacggattca
      241 gcaaaagaat cagcggaatg gcatcctcgc caagattgaa tctttctgcg atcactgcca
      301 gagagtctcc atcttgtact gtatatgtaa atggagctga gaaggtcggt gtgaaggtaa
      361 tggtgggcga aggagtttct gtgaatgttg gtgtaggtgt atttgtattt gttggcgtat
      421 aggtgagtgt tggggtaggg gtttcagttg ccaacaacag gttgatgggt ttgccgggga
      481 gagtcatcca atagatcagc aatgcgagac ctcccaacag gagaacgccg gccacaataa
      541 cgatggcgtt ctgcctgccg cggtttcgtc tgcggtagga gccaatggtt ccagaaggag
      601 gggtgcttgg agttctgaga gtcatttaat caatcctttt gcacgtcggt ctaaatgtat
      661 tttccaacca attgcagtgc aaaataattc tacctgaaaa aggggaatcg cgtgtgcatg
      721 gcacaaaaaa acaggctgac attgccagcc tgttttcaaa atgctctatt agcggtttat
      781 atggtctttc aagtatttgc ccgtataaga gcctttcacc ttcatgactt gttctggagt
      841 gccttcagca atcaactcgc cgccacggtt accgccttct ggaccaaggt caatcagcca
      901 atctgccact ttgataatgt ccagattatg ctcgatgata agcaccgagt tgcccgtgtc
      961 aacgagccgc tgcatcactt caatcaactt gtgaacgtct gctgcatgta aaccgacaga
     1021 aggttcatcc aatacgtaaa gtgtacgtcc cgttgcccgg cgagaaagtt ctttcgagag
     1081 tttcacgcgc tgtgcttcac cccccgagag tgttgttgcg ggttgaccta cccgcacata
     1141 tcccaaccct acttcctgca agagcttcaa cttgttctgc atttgtgggt agttcttgaa
     1201 ttcttccagt gcatggtcta ccgtcatatc gagtacatca gcaatcgaat acgtatcacg
     1261 gaacatcacc tgcaaggtct cgcggttgaa gcgtttgccg tgacacacat cgcaaggcac
     1321 atacacatct ggcaaaaatt gcatttcgat gcgtaactca ccttgtccct gacaggcttc
     1381 acagcgcccg ccatgaacat taaaggagaa acgccctggt ttgtacccgc gcactttaga
     1441 ctcaggaagt tctgcaaaca acttacgaat ttcatcaaac aagccagtgt atgtaccggg
     1501 attcgaacgt ggcgtgcgtc cgatagggga ttggtcgatg ttaataatct tatcgagatg
     1561 ttcaattcct tcaatcgttt tatgctcgcc agattgtgtc cgcgcgccca tgagttttgc
     1621 cgcaagttcg ttatacagga tgtcgctcat cagcgtggac ttgcccgagc cggagacgcc
     1681 tgtaatacaa actaatttcc caagagggat attgacatca atgcccttca aattatttgc
     1741 tgttgcatta acaattttga gcaacttgcc attgccttcg cgcctctttt tcgggatctc
     1801 gacctgtttg cgccccgaga gatagagtcc agtcagtgac tttgggtgcg ccaaaatctg
     1861 cttcggtgtg ccttctgcaa taatttgccc accgtgttcg cccgcagcag gacctaagtc
     1921 gatgacccaa tctgattcaa gaatagtctc atcgtcatgt tcaacgacaa gaaccgtatt
     1981 tcccagatcc cttaaccctt tgagcgtttc aagcagacgg gtgttatcac gcgggtgcaa
     2041 accaatggaa ggttcatcta aaacgtatag aacgcctacg agacgagaac caacctgagt
     2101 tgcaaggcgg atgcgttg
//
LOCUS       DAOQJE010000697         2110 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_41473_length_2110_cov_4.063260, whole genome shotgun sequence.
ACCESSION   DAOQJE010000697 DAOQJE010000000
VERSION     DAOQJE010000697.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2110)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2110)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2110
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41473_length_2110_cov_4.063260"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..921
                     /locus_tag="PLT08_15395"
     CDS             <1..921
                     /locus_tag="PLT08_15395"
                     /inference="COORDINATES: protein motif:HMM:NF012706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HNE05910.1"
                     /translation="VLGISQITQDVLDAALQEPGLSIYTSRLPQMGLVFLNTNNPAVG
                     FMQDAKVRRALMLGLNRTVIVSHILKGQAIVADSPILPGSWAYYDEIEKFPYDPETAA
                     QILNKEGYVFTAGNSIRSKDGQSLSFTLVHPDDAIHTQIAQAIQGDWALIGVEVKLQS
                     VPYDSLVNEYLNSRNYEAALADLNTSRTPDPDPYLFWHQAEATGGQNYSQWDDRTASE
                     FLETARTTADFNERIRLYNNFQVVFAKELPSLPLYYPVYSYGVDSQVQGVQVAPMYDV
                     SDRLSLINEWYLVTRRTLEEQSTAEPAATP"
     gene            984..>2110
                     /locus_tag="PLT08_15400"
     CDS             984..>2110
                     /locus_tag="PLT08_15400"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: protein motif:HMM:NF006053.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase"
                     /protein_id="HNE05911.1"
                     /translation="MTDSQKAVEYVRSNNERFLNELKDLVAIPSISTLDENKSDVQRA
                     AEWVAGQLRSIGMDNVKIMPTALHPVVYGEWMGAGKSAPTVMIYGHYDVQPVDPLELW
                     TSDPFKAEVRGDYLFARGASDMKGQVVASIKAVEAIMKTEGKMPVNIKWLVEGEEEIG
                     SEHLGDFIKNNKELLAADFCLNPDAGMIGPDKPTITTGLRGLAYFELRVFGPDKDLHS
                     GLFGGTVHNPAQALIELIAGMHDKNGKITLPGFYDKVRKLSKKEREDFKRLPTTNKDL
                     IKMTGVPALWGEPQFIPAERVGARPTLEVNGLLSGFTGQGSKTVLPAWAMAKISCRLV
                     PDQTPEQTEKQLRAYLKKHAPKTIKWELKNLHNAGAALTEV"
BASE COUNT          531 a          572 c          527 g          480 t
ORIGIN      
        1 gtattgggca ttagccaaat cacacaagat gtcttggatg ccgccttgca agaaccgggg
       61 ctttccattt acaccagccg cctcccgcaa atgggattgg tcttcctcaa caccaacaac
      121 cctgccgtgg gctttatgca agatgcaaaa gtccgccgcg cgttgatgtt gggactgaat
      181 cgcaccgtga ttgtctcgca catcctcaaa ggtcaagcca ttgtggcaga tagccccatt
      241 ttgccgggct catgggcgta ttacgatgag atcgaaaaat tcccatacga ccccgaaaca
      301 gcggctcaga tcttgaataa agaaggctat gtcttcactg cgggcaatag catccgctcg
      361 aaagatgggc aatcgctttc cttcaccctc gtccacccag atgacgccat ccacacccag
      421 attgcgcaag ccattcaggg cgattgggcg ttgatcgggg tggaggtcaa gttacagtct
      481 gtgccttatg actcgctggt gaatgaatat ctcaactcgc gcaattacga agccgccctg
      541 gcagacttaa acacctcgcg cacgccagac ccagacccct atctcttctg gcatcaagcc
      601 gaagccacgg gtgggcagaa ctattctcag tgggatgacc gcactgcgag cgagttcctc
      661 gagaccgccc gcaccactgc agatttcaat gagcgcatcc gcttgtacaa taacttccaa
      721 gtggtcttcg ccaaggaatt gccgtctttg cctttgtatt acccggtcta ttcctatggc
      781 gtagattcgc aagtgcaagg tgtacaagtt gcaccaatgt atgatgtcag tgacaggttg
      841 tctctgatca acgaatggta tttggtcaca cgccgtacgc tcgaagagca atcgacggct
      901 gagcctgccg caacaccgta acccaacaga ccctaaaggc attcaaagcc tttagggtca
      961 ttcatactat aaggagtttc atcatgactg actctcagaa agctgttgaa tacgtccgct
     1021 cgaataatga aagatttttg aacgagttga aggatttggt cgccattcct tcgatctcta
     1081 ctttggatga gaacaaatca gatgtgcagc gcgccgccga gtgggtggcg ggtcaactgc
     1141 gctccatcgg catggacaat gtgaagatca tgccgaccgc tttgcatccc gtcgtctatg
     1201 gcgaatggat gggggcgggc aaatcggctc ccaccgtgat gatctacgga cattatgacg
     1261 tccaacccgt cgacccgcta gagctttgga cgtctgaccc gttcaaggcg gaggtgcgag
     1321 gcgattattt attcgcacgt ggcgcttcgg atatgaaggg tcaagttgtg gcatccatca
     1381 aagcggtgga agccatcatg aaaaccgaag gcaaaatgcc ggtgaacatc aaatggttgg
     1441 tggaaggcga agaagaaatt ggctccgaac atcttggcga tttcatcaag aacaataaag
     1501 aattgcttgc cgctgatttt tgtttgaacc ccgatgccgg catgatcgga cccgataagc
     1561 ccaccatcac aaccggtttg cgcggtctcg cttattttga attacgcgtc tttggtcccg
     1621 ataaagattt gcactccggc ttgttcggcg gcaccgtcca caacccggct caagcgttga
     1681 tcgaactcat tgccggcatg cacgataaga atggcaaaat cacgttgcca ggcttttacg
     1741 acaaggtccg caagttgagc aagaaagaac gcgaagattt caaacgcctg cccaccacca
     1801 acaaagacct catcaaaatg accggcgtgc ctgctctgtg gggagaacct caattcatcc
     1861 ctgccgaacg tgttggagct cgccctactc ttgaagtgaa tggcttgctc tccggtttta
     1921 ccggtcaggg ttctaagacc gttttgcctg cctgggcaat ggcgaaaatt tcctgccgtc
     1981 tcgtgcccga ccaaacgcct gaacaaaccg agaaacaatt gcgcgcctat ctcaaaaaac
     2041 atgcgcccaa gaccatcaaa tgggaactga agaatctgca caatgctggc gccgcattaa
     2101 ccgaagttga
//
LOCUS       DAOQJE010000698         2105 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_41665_length_2105_cov_4.136585, whole genome shotgun sequence.
ACCESSION   DAOQJE010000698 DAOQJE010000000
VERSION     DAOQJE010000698.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2105)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2105)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2105
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41665_length_2105_cov_4.136585"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..439)
                     /locus_tag="PLT08_15405"
     CDS             complement(<1..439)
                     /locus_tag="PLT08_15405"
                     /inference="COORDINATES: protein motif:HMM:NF015898.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HNE05912.1"
                     /translation="MTEKYLLDAAEMIGSGYEPSVEIESPRIISERRDGKLVDVERTA
                     FIKLYTSFKRELKEMEGNDLKVWVYLALSVNRYSKTANPGLRKIAEETGLAVNTVRSC
                     VERLEEKGLLGTEKEAGKGTNYRPADYVSVSKNDTVTVSKFDTT"
     gene            complement(442..783)
                     /locus_tag="PLT08_15410"
     CDS             complement(442..783)
                     /locus_tag="PLT08_15410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE05913.1"
                     /translation="MKTELTELQETILKIVSKAKKPATRPSIQFAVYGTTNNRNNDRA
                     IQNAIKALRDMGYVIVSNSDHSGYKLATTQAEVDAYVAERKKAAAQMIKTAMKVRKAF
                     QQRGQMRMAGA"
     gene            complement(1074..1343)
                     /locus_tag="PLT08_15415"
     CDS             complement(1074..1343)
                     /locus_tag="PLT08_15415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE05914.1"
                     /translation="MQTKTRSNLGMKAGMKVVKTTSNVRRLCDQKGWSKDDFIGHMML
                     AGVKPITSKRIYDGETNIRVNTAALVAHLLGVSLGDVIEIDHSKN"
     gene            1511..1699
                     /locus_tag="PLT08_15420"
     CDS             1511..1699
                     /locus_tag="PLT08_15420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE05915.1"
                     /translation="MSNTNTATVGNPLSVRRAEILEAIKNKTYAPGQLTELTKELAEI
                     EAQLSREWTVEEILEREG"
     gene            1702..2046
                     /locus_tag="PLT08_15425"
     CDS             1702..2046
                     /locus_tag="PLT08_15425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE05916.1"
                     /translation="MSAIGINNGNFRTGDYKAWATNDLSNVPTFTQYCDVKKAVRESF
                     GTQEAYDAWEKDAPKYDAFFAYAEDYLIQIKLEDAKIIAEHKDWLDEKATETSATGAG
                     WFSFADIQEIPF"
BASE COUNT          526 a          545 c          475 g          559 t
ORIGIN      
        1 cagttgtgtc aaattttgat acagttactg tgtcattttt tgatacactc acgtaatcag
       61 cggggcggta gtttgtcccc tttccagctt ctttttcggt tccaagaagc cctttttctt
      121 caaggcgctc cacgcatgac ctgacggtgt ttacagcgag tccggtttcc tctgcgatct
      181 ttcttagtcc agggtttgcc gtctttgagt atctgttcac gctcaaggca agataaaccc
      241 aaaccttcaa gtcgttccct tccatttctt tgagttcacg cttgaatgat gtgtacagct
      301 tgataaatgc ggttcgctct acgtccacta atttaccgtc gcgtcgctcc gaaataatgc
      361 gcggggattc gatttctact gacggttcgt atccgcttcc aatcatttct gccgcgtcta
      421 atagatattt ttctgtcatt cctacgcccc cgccatgcgc atctgcccgc gctgctgaaa
      481 tgccttgcga accttcatcg cagttttaat catctgcgcc gcggctttct tgcgctcagc
      541 aacataggca tccacttccg cttgcgtggt cgccagctta taacctgagt gatccgagtt
      601 cgagacaatc acatagccca tgtctcgcag tgccttgatt gcgttctgaa tggcgcggtc
      661 attgttgcgg ttgttcgtgg tgccatatac cgcaaactgg atgctcgggc gggtggctgg
      721 cttcttcgcc ttgctcacaa tcttgagtat agtttcttgc agttcggtta attcggtttt
      781 catttcttct ccttttcttg attcaacatt ttgattaata ctcgcttcat gtcttgcgct
      841 ctcagcgtta ccagccccgc ctttgctccc cgcctcgtgg aagcggggag ggtggtcatc
      901 agccaccaaa tcaaggaggg agagaaacgg aaactacgcc ggtcttccca tcaggaagtg
      961 gcggggtttc aaaaataaaa tagccagcac gcacaacaag gcgccggcaa tgataatagc
     1021 aatgggggtc ttcaacaaac ggaaagccat tacgagactc tggtattgac ttgttaattt
     1081 ttagagtggt caatttcgat gacgtcacca agactgactc caagcaggtg agcgactagc
     1141 gcggcggtgt tcacgcggat attcgtctca ccgtcataaa tccgcttgct tgtaataggt
     1201 ttcacgcccg caagcatcat gtgaccaata aagtcgtcct tagaccaccc cttttggtcg
     1261 cacaggcggc ggacattact ggtggttttt accaccttca tgcctgcctt cattcctaag
     1321 ttagagcgag tttttgtttg catgtgtaat agcttacaac cttattcaat ttttgtcaag
     1381 tttttataat ataaagatgt tatagaaaac cttgacaata tcaaaacatg cgcttatact
     1441 tccctcactg tcatcaacag tattaccgca tcgccatcag cgggcggcac atcaaaaagg
     1501 agatgaacgc atgagcaata caaacactgc aacagtgggg aatccgcttt ctgtgcgtcg
     1561 cgcagaaatt ctcgaagcta tcaaaaacaa aacctacgcc ccagggcaac tcaccgaact
     1621 gaccaaagaa ctcgccgaga tcgaagcgca attgtcgcgt gagtggactg tcgaagaaat
     1681 cctcgagcgg gaggggtaat catgtccgca atcggtatca acaacggcaa ttttcgcaca
     1741 ggcgattaca aagcatgggc tacgaatgac ctgagcaacg tcccgacctt tacgcaatac
     1801 tgcgacgtaa aaaaggcggt ccgcgaaagt ttcggcacgc aggaagccta cgacgcatgg
     1861 gaaaaagacg ccccgaagta cgacgccttt tttgcctacg ccgaagatta tctgattcag
     1921 atcaaactcg aagacgctaa gattatcgcc gagcataaag actggctcga tgaaaaagca
     1981 accgaaacaa gcgctaccgg tgctggctgg ttctcattcg ccgatattca agagatacct
     2041 ttttagggaa taaccattat gcaacccaca accgaaattc aaaaaaccga agccaaagaa
     2101 ttggc
//
LOCUS       DAOQJE010000699         2100 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_41836_length_2100_cov_6.728606, whole genome shotgun sequence.
ACCESSION   DAOQJE010000699 DAOQJE010000000
VERSION     DAOQJE010000699.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2100)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2100)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2100
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41836_length_2100_cov_6.728606"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            38..730
                     /locus_tag="PLT08_15430"
     CDS             38..730
                     /locus_tag="PLT08_15430"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HNE05917.1"
                     /translation="MAFTFYRIPVGICNSFLLRGDRTILIDGGAMGGFPACQRQLKAL
                     NVDPKEIELILLTHGHWDHITCLADIQKLTGAKIAIHGRDQFMVETGEPPFPDGATPY
                     GNAMSWLSKRVLHPHLPPLKVDTVILDAGMSLKEFGIPGEVIYTPGHSMGHVSILLET
                     GDALVGDMAMNDWYLRWTPGLPVLADNIEMVVDSWKKILPMGIKRVYPAHGKDFDIEV
                     MKKEIATFKLTK"
     gene            complement(767..1015)
                     /locus_tag="PLT08_15435"
     CDS             complement(767..1015)
                     /locus_tag="PLT08_15435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE05918.1"
                     /translation="MSAENDIIALRSRVAELERKLDFLYRRMGIEYVDDPGMIDSQII
                     SLLKKGNKLEAIKVYRELTNTDLADAKRAVEKIEASIL"
     gene            complement(1081..1710)
                     /locus_tag="PLT08_15440"
     CDS             complement(1081..1710)
                     /locus_tag="PLT08_15440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE05919.1"
                     /translation="MKNQTPLLLTVSLILLLVASCTPKATQDPNLLIQQAVNATLAAI
                     PTATQVSPPTPYPSPTPFSLAGLFCEYQFCIGHPIDVSFFDVSAQQNPASPSTYGQGI
                     LAALNGNLFIQVMWQLAPGTSDPKFLFDTMLNDSIDTATNTSSIFLVRDMNVFYDDIT
                     TLATPSLPYGGAGAWTCGDRVFAWKVYTPQSGTARGMFDEMLARFTCGQ"
     gene            complement(1766..>2100)
                     /locus_tag="PLT08_15445"
     CDS             complement(1766..>2100)
                     /locus_tag="PLT08_15445"
                     /inference="COORDINATES: protein motif:HMM:NF019351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="multicopper oxidase domain-containing protein"
                     /protein_id="HNE05920.1"
                     /translation="LKANVGESIRIFFGVGGPNFTSSFHVIGEIFDRVYDQASLTSAP
                     LTNVQTTLVPPGGATMVEFNVEVPGNYILVDHALSRLQRGLAGVLIVEGDQNPEIYNG
                     VPSAGSGH"
BASE COUNT          507 a          453 c          600 g          540 t
ORIGIN      
        1 gcatacatct catccgaatg ggtacggaaa ataaaatatg gcattcacgt tttaccgtat
       61 tccggttggg atctgtaatt cgttcctctt gcgcggtgac cgtacgatct taatcgatgg
      121 tggagcgatg ggcggcttcc ctgcatgcca acgtcaactt aaggcgttga atgttgaccc
      181 caaagagatc gagctgattc tccttacgca tgggcattgg gaccacatca cttgccttgc
      241 agatattcag aaactgaccg gagcgaagat cgcgattcat ggacgggatc agtttatggt
      301 cgagacgggc gagccgccat ttccagatgg tgcgacaccc tatggaaacg ccatgtcgtg
      361 gctttcgaag cgggtactac atccgcatct tcccccgctc aaagtggata cggtgattct
      421 ggatgcgggc atgtcgctga aagagttcgg cattcccggc gaagtgatat acactccggg
      481 gcattcaatg ggacatgtaa gtatcttgtt ggaaacgggt gatgctttgg taggggacat
      541 ggccatgaac gattggtacc tgcgttggac gcccggactg cccgtgcttg cggataatat
      601 cgagatggtt gtggatagtt ggaagaagat cttgccaatg ggcatcaaac gggtctaccc
      661 cgcacatggc aaggactttg atattgaagt gatgaagaaa gagatcgcca cgttcaagct
      721 tacaaaatga aacaaaaacg ccagagattt tctctggcgt ttttgtttat aaaatgctgg
      781 cttctatttt ttcgaccgct cgtttggcat ctgccaaatc ggtattggtc aattcacggt
      841 agaccttgat ggcttcaagt ttgttacctt ttttgagcag ggaaatgatc tgtgaatcga
      901 tcatgccggg gtcatcaaca tattcaattc ccattcttct ataaagaaaa tcgagctttc
      961 tttcaagttc agcgacgcgt gagcgcagtg caataatatc gttttcagca gacatagctt
     1021 atctccttca aaacgatatt acacccgctc gttcttgctg acattcacct atatttccta
     1081 ttactgtcca caggtgaagc gggctagcat ttcatcgaac atgccacgag cggtgcctga
     1141 ttgaggggtg tataccttcc aggcgaagac tcggtcgccg caggtccagg cgccagcgcc
     1201 gccatatggt agggacggcg ttgccagagt ggtaatgtca tcgtagaaca cgttcatatc
     1261 gcggactaga aaaatgctgg aggtgtttgt ggcggtatca atgctatcgt ttagcatcgt
     1321 atcgaacaga aacttagggt ctgaggtgcc aggggcaagt tgccacatta cttgaatgaa
     1381 tagattgccg ttgagcgcag ccaatatgcc ttgcccataa gtgctaggag atgctgggtt
     1441 ttgttgggcg cttacatcga aaaaggaaac atcgatggga tgaccgatgc agaattgata
     1501 ttcgcagaat aagccagcca aactaaaagg tgtgggcgag ggataaggtg tgggtggcga
     1561 cacttgtgtt gcagtgggaa tggcggcaag agttgcgttg accgcttgtt ggatgagcag
     1621 gtttgggtct tgagtcgctt tcggtgtgca ggaagcaaca agaagcagaa tcaggctcac
     1681 cgttaagaga agaggcgttt ggtttttcat ccgcgaaatt ataaaggaaa agccccgtcc
     1741 aatttcgacg gggctttggt ttggtctagt gtccgctgcc tgcagatggg acaccgttat
     1801 aaatttctgg gttttggtcg ccttcgacga tgagcactcc ggcgagaccg cgttgtaatc
     1861 tggataaggc gtggtctaca aggatgtagt tgccgggcac ttcgacattg aactcaacca
     1921 tggtagcgcc tccgggcggg acgagggtgg tttgtacgtt ggtgagtggc gcgctggtga
     1981 gagaggcttg atcgtagacg cggtcgaaga tttcaccgat gacgtggaaa gaggatgtga
     2041 agttcggtcc gccgacaccg aagaagatgc gaatgctttc gccgacattg gctttgagcg
//
LOCUS       DAOQJE010000700         2095 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_42044_length_2095_cov_4.739706, whole genome shotgun sequence.
ACCESSION   DAOQJE010000700 DAOQJE010000000
VERSION     DAOQJE010000700.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2095)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2095)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2095
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42044_length_2095_cov_4.739706"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..620
                     /gene="rsgA"
                     /locus_tag="PLT08_15450"
     CDS             <1..620
                     /gene="rsgA"
                     /locus_tag="PLT08_15450"
                     /inference="COORDINATES: protein motif:HMM:TIGR00157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HNE05921.1"
                     /translation="VFVFACANPNPKLRMLDRFLIIAEKQKLPVVIVANKIDLVENPK
                     DNFGMYEAIGYHVVYTSTKNETGLAELRNQLRNKLSAFAGPSGVGKSSLLNALQPGLG
                     LAVNEISSVMNKGRHTTVVRQMFAFEGGYVADTPGWKSLGLWDTEPEEIDAYFPELRG
                     LVEQCQFSDCTHQHEPGCAVLTALKDGKIHAERYESFVRLRSGKE"
     gene            699..1706
                     /gene="holB"
                     /locus_tag="PLT08_15455"
     CDS             699..1706
                     /gene="holB"
                     /locus_tag="PLT08_15455"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HNE05922.1"
                     /translation="MTDNWNLLGHEWAVDMLRRHVARGEMRHAYLFAGAPGLGRRTLA
                     LRLAQALNCEKPVAPGEPCFVCRTCKQIDAMQHPDLAIIQAVDEDGVGKDGGTLRVDQ
                     IREVQRTLNLKPYQSPYRVAIFLRFQEANDNAANALLKTLEEAPAHAILLLTADNPEQ
                     LLPTINSRCEILRLRPLPLETIVADMLDHGVDEDRARLLAHISGGRPGFARKLLEDAT
                     VLEKREERLNDLQTILPASRVEKFSYAEKLAKDKDAMRQTILVWLSYWRDVLLRVAGA
                     ETPLINVDRNMEIEFLAGRLNLSTARKVVSDHESVLEKMERNVNSRLLAEVLLMDLPK
                     M"
     gene            1735..>2095
                     /locus_tag="PLT08_15460"
     CDS             1735..>2095
                     /locus_tag="PLT08_15460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE05923.1"
                     /translation="MKKQNILFWASVAISALYGLIVTGFGIYLAGKGQDCLSYNDYAC
                     AFSGMFSFVIIPYGLAILLFVGIASLPFPVARLIGSILSALIGMGSVGLCCIVVLGAT
                     TTISTDGAILTGSEMAFF"
BASE COUNT          528 a          459 c          528 g          580 t
ORIGIN      
        1 ctgtcttcgt cttcgcatgc gcgaatccaa atcccaaatt gagaatgctc gaccgttttc
       61 ttattattgc agaaaagcaa aaacttcctg tggtaatcgt ggcgaataag attgacttgg
      121 ttgagaatcc aaaagataat ttcggaatgt acgaagctat tggttatcat gtcgtttata
      181 catccaccaa aaatgagaca ggtcttgcag agcttcgtaa tcaattgcgg aataaactca
      241 gcgcctttgc cggacccagc ggcgtgggca agtcgagttt attgaatgcg ctacaacctg
      301 gtttggggtt ggctgtgaac gagattagtt ctgtgatgaa taagggacgt cacaccacag
      361 tggtgcgcca aatgtttgcc tttgaaggtg gctatgttgc agatacacct ggttggaagt
      421 cccttggctt gtgggatacc gaacccgaag agatcgatgc ttacttccct gagttgcgcg
      481 ggctggtaga gcaatgtcaa tttagtgatt gcacccatca acatgagccg ggttgtgccg
      541 tgctcacagc attgaaagat ggcaagattc atgcagaacg atatgagtct tttgtaaggc
      601 tccgttctgg caaagaataa cgcgtagaaa attaccaaat acgtattgca tgctttccac
      661 acttgacact taatctctga cccctgatac ttaaaccaat gactgataac tggaatttac
      721 tcgggcacga atgggcagtg gatatgcttc gccgccatgt agcgcgtgga gaaatgcgcc
      781 atgcctacct ttttgcaggg gccccgggtt tgggtcgccg gacgttggca ttgcgcctag
      841 cacaagcttt gaactgtgag aaacctgttg caccgggaga gccttgtttt gtgtgccgca
      901 cttgcaaaca gatcgacgcc atgcaacacc cagatttagc gatcattcaa gccgtagacg
      961 aagatggagt ggggaaggat ggcggcacct tgcgcgtgga tcagatccgc gaagtgcagc
     1021 gaacgttgaa ccttaagccg tatcaatctc catatcgcgt ggctatattc ttacggtttc
     1081 aagaagcaaa tgataatgcc gccaacgcct tgctcaaaac attggaggaa gccccggctc
     1141 acgctatttt gcttctaact gcagataacc ccgaacagct tttaccaacc atcaactcgc
     1201 gttgtgaaat tttacgtttg cgcccgttgc cgctcgaaac catcgttgca gatatgcttg
     1261 atcacggcgt ggacgaagat cgtgctcgtt tgttggcgca catctcgggt ggtcgccctg
     1321 ggtttgcgcg taaattgctg gaagatgcca ctgtccttga gaagcgcgaa gaacgtctca
     1381 atgatttgca aaccattttg cctgcatcac gcgttgaaaa attttcttat gccgagaagc
     1441 ttgctaaaga taaagatgcc atgcgccaga ccatccttgt atggctttct tattggcgag
     1501 atgttctttt acgagtagca ggtgcagaga ctccgcttat caatgtggac cgcaatatgg
     1561 aaattgaatt cctagcagga cgcttaaatc tctccaccgc gcgaaaagta gtcagcgatc
     1621 atgagtccgt cttagaaaag atggagagga atgtcaattc aaggttgttg gcagaagtat
     1681 tgttgatgga tttgccgaag atgtaagttt tagttttata aaaaataaag gaaaatgaaa
     1741 aaacaaaaca ttcttttttg ggcgtcagtg gcgatttctg ctctctatgg gctcattgtc
     1801 accggatttg gaatttatct ggcaggcaaa gggcaagatt gcctgagcta taacgattat
     1861 gcctgtgcct tcagtggaat gttcagtttt gttattatcc cctatgggct tgccatttta
     1921 ttgttcgttg gaattgcaag cttgcctttt cccgttgccc gtctgattgg atccattctg
     1981 tctgcattga ttggcatggg aagcgttggc ttgtgctgta ttgttgtcct tggggcaacg
     2041 accacaataa gcacagacgg cgctatttta acaggcagtg aaatggcttt ctttc
//
LOCUS       DAOQJE010000701         2093 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_42115_length_2093_cov_6.868499, whole genome shotgun sequence.
ACCESSION   DAOQJE010000701 DAOQJE010000000
VERSION     DAOQJE010000701.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2093)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2093)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2093
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42115_length_2093_cov_6.868499"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            71..760
                     /locus_tag="PLT08_15465"
     CDS             71..760
                     /locus_tag="PLT08_15465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE05924.1"
                     /translation="MLDKIKSNFNTQTWVLIPIAIAINLAIGQIVLVLKLPVFLDSIG
                     TVMVGVLCGPWAGALTGALSNTIAGIALDPGWFPWWPVAAMIGYMAGRMAGWGFFKSW
                     WRVAVTGFVIALTAAIVSTPIAVYLFGGITASGSSFITAYLLATGQGIWSAVVSTSFL
                     TEPVDKITTAMIAFAIIQGLPKRTIGGYPKADQVAAEGGESKNQLYIAVGVIVVLVLF
                     AAFMLRNLLGG"
     gene            837..1616
                     /locus_tag="PLT08_15470"
     CDS             837..1616
                     /locus_tag="PLT08_15470"
                     /inference="COORDINATES: protein motif:HMM:NF014420.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     component T"
                     /protein_id="HNE05925.1"
                     /translation="MHERLSFHIQRDSLLHRLNPLTKMVIILTIILISFTFPYYWTPH
                     LLLLIVILPLSLTGKVFREFSLTALRLIIPAAGFLFLMQAFFQPIGETVIFKFYFLDM
                     TMESVMFAFRNAMRIVVMVSGFTIFLLTTHPSELMSDLTRRGLPGQFAYVIISTLQIL
                     PQMQAKAQTIISAQRSRGLDTESSFLKRAGSLIPLIGPLVFGSLVEVEERAIAIEARG
                     FTSKQVKTSLHEIPDTASEKTLRWGLLALIVISIGLNLWLS"
     gene            1604..>2093
                     /locus_tag="PLT08_15475"
     CDS             1604..>2093
                     /locus_tag="PLT08_15475"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNE05926.1"
                     /translation="MAFVNIQNLTYKYPLVKEPVLRNITAQIQEGEFVSIVGPNGAGK
                     STFCYALAGFAPHFFKGEFSGEIEVSGKASSKSTLDEWVLNVGLAFQNPFNQISGAKY
                     TVFEEIAFGLENIGVPREEIKSRVQDAMSLTGISELADRSPYSLSGGQQQRVALTSIL
                     VMR"
BASE COUNT          469 a          561 c          479 g          584 t
ORIGIN      
        1 tcgtgacaat ctgaaaaata cagtgttatc attgctgtag ctttataact caattcactc
       61 ggaggatagc atgctggata aaatcaagag caatttcaac acgcaaacgt gggttctcat
      121 ccccattgcc atcgcaatta atcttgccat tggtcaaatt gtgctagttc ttaagttgcc
      181 ggtcttcttg gatagcattg gcaccgtaat ggttggtgtg ctttgtggtc cttgggctgg
      241 tgcgcttact ggtgccttat ccaataccat tgcagggatt gcgctcgacc caggttggtt
      301 cccttggtgg cccgttgccg caatgatcgg ctacatggcg ggacgcatgg ctggctgggg
      361 cttctttaag agttggtggc gcgtggctgt gaccggtttc gtgatcgccc tcaccgctgc
      421 gatcgtctcc acccccattg ccgtgtatct ctttggcggt atcactgcca gcggctcttc
      481 cttcattact gcatacctgc tcgctaccgg gcaaggcatt tggtcggctg tagtcagcac
      541 cagcttctta accgagccgg tcgataaaat caccactgcc atgatcgcct ttgccatcat
      601 tcagggcttg cccaagcgca ccattggcgg ctatcccaag gcagatcagg tcgcagccga
      661 aggcggcgaa agcaagaatc agttgtacat cgccgtgggt gtgatcgtcg ttttggtgtt
      721 gttcgcagcc tttatgctca gaaaccttct gggcggctaa actaaagctc gcttttcaaa
      781 aagagaccag acattctggt ctcttttttg ttgccaagtg ggtataatcc caccctatgc
      841 atgagcgcct ttccttccac atccaacgag acagtcttct gcatcgtctc aacccgttga
      901 ccaagatggt gatcatccta accatcattt tgatctcctt tacattcccc tattactgga
      961 cgccccatct gcttttattg atcgtcattc ttccgctcag cctaacggga aaagtgtttc
     1021 gcgaattttc cctcacggcg ctgcggctca tcatccctgc ggctggtttt ctttttctca
     1081 tgcaagcctt cttccagccg attggcgaaa cagtgatctt caaattctat tttctcgata
     1141 tgaccatgga aagcgtcatg ttcgccttcc gcaatgccat gcgcattgtg gtcatggttt
     1201 caggtttcac catctttttg ctaactacac accccagtga gttaatgtcc gaccttactc
     1261 ggcgtggatt gcccggtcag tttgcttacg tcatcatctc tacactgcaa attttgccgc
     1321 aaatgcaagc caaagcacaa accatcatct ccgctcagcg ctcacgcgga ctcgacacag
     1381 aaagcagctt tctcaaacgc gctgggtctc tcattccgct gatcggtccg cttgtgttcg
     1441 gctccttagt cgaagtggaa gaacgtgcta tcgccattga agcgcgcggc tttacatcca
     1501 aacaggtcaa aacctctttg catgaaattc cagatacagc ctctgaaaaa actttacggt
     1561 ggggcttgct cgcattaatc gtcatttcca ttggattgaa tctatggctt tcgtaaacat
     1621 tcaaaacctc acatacaaat atccacttgt caaagagccg gtgctacgaa acattacggc
     1681 acagattcaa gaaggtgaat tcgtttccat cgttggtcca aatggcgcag gcaaatccac
     1741 gttttgttat gcattggctg ggtttgcgcc gcactttttt aaaggcgaat tttcaggcga
     1801 aatcgaagta agtggaaaag cctcctccaa gtccacatta gacgaatggg tcttaaatgt
     1861 tggcttggcg tttcaaaatc ccttcaatca aatttccggc gcaaaatata cggtctttga
     1921 agagattgct ttcgggttgg aaaacatcgg tgtgccgcgt gaagaaatca aaagccgcgt
     1981 acaggatgcc atgtccctga ccggaatcag cgagttagcc gatcggtctc cctactctct
     2041 ctctggcgga caacaacaac gcgtcgcgct gacctcgatc ctggtcatgc gac
//
LOCUS       DAOQJE010000702         2091 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_42229_length_2091_cov_2.821218, whole genome shotgun sequence.
ACCESSION   DAOQJE010000702 DAOQJE010000000
VERSION     DAOQJE010000702.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2091)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2091)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2091
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42229_length_2091_cov_2.821218"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..409)
                     /locus_tag="PLT08_15480"
     CDS             complement(<1..409)
                     /locus_tag="PLT08_15480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE05927.1"
                     /translation="MAKIAQALDRLFEKYRIVFWYDEKKELRGAFESLLIPGVETIEL
                     KNNEYGVKYHILREKPAQKFLLYHEGPQPDDLDNWLLDVQLASGLFNANQEALWANEI
                     GLQPQLFDLVTSHIEFFKDEKRRAALKARYKQED"
     gene            complement(435..>2091)
                     /locus_tag="PLT08_15485"
     CDS             complement(435..>2091)
                     /locus_tag="PLT08_15485"
                     /inference="COORDINATES: protein motif:HMM:NF016309.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="nuclease domain-containing protein"
                     /protein_id="HNE05928.1"
                     /translation="KRQLSGDAFENLAKITEENWENIKNNPAKEIKLGTKIVDLTNLQ
                     SAQSIRSMNQSVQKPHQRRQLIVERQETYDSEENRFLVHVLKDILIPKAKPLMNYFRL
                     QAAKIKEIQRKPDGYRHGQNYLHLWQERRVNIEQEANRLESQAANIEGIINKAEGYLQ
                     EPFLRNITSNVSSFRPSAKIADSKVYGPIYRAYQDYLSNAKSSRQKSLAWALEEKSVR
                     RVSKLYEIWVFFEVYARLVHDFGFLPDGDSPFDTIEVYDEDINLREGSEYRLSFVPKK
                     NYFSKVPVCTAVLSYSPKIKPRPCKLGKKCFDQNICPSLPCYNEINTGTWEELKPDIT
                     LTLQTGAQIKKIALDVKYRNYADMKYVSEKDKKYMVNTPFEADIFGTAKMKYLNGLEC
                     DASFVLHSDPNPQYTVFGGEPFTPTPLRERANGEWWPGHKIGAVCVTPSHQKGLDSLL
                     RCFLMYHARLVSICWNPLCHSILTEGNGMMKQPGYKGDYYQCPDCGEFWISQYCSNKT
                     QHHPLVKMGKASFHKTASQNEWNCVCPACGDQFTADSKIPVKQ"
BASE COUNT          538 a          474 c          424 g          655 t
ORIGIN      
        1 tatcctcctg cttgtagcgg gctttaagcg cggcacggcg tttttcgtct ttaaaaaatt
       61 caatgtgact ggtgaccaga tcaaagagct gcggctggag tccgatctcg tttgcccaga
      121 gcgcttcttg attggcgttg aacaaaccgc tcgccagttg cacatccagc agccaattat
      181 ccaaatcatc gggctgggga ccttcatggt agagcaggaa tttttgtgcg ggtttctcgc
      241 gcaggatgtg atatttgacc ccgtattcgt tgttcttgag ttcgatggtt tcaaccccag
      301 gaattagcag agactcaaaa gccccccgaa gttctttttt ctcatcgtac cagaatacga
      361 tgcggtattt ttcgaataga cggtcgaggg cttgggcgat tttggccatt aggtaatttt
      421 acctgccatt cctgttattg cttgactgga atttttgaat ctgctgtaaa ctgatcccca
      481 cacgcagggc aaacacaatt ccactcattc tgggatgctg ttttatgaaa agaggctttg
      541 cccattttta ccaatgggtg gtgttgcgtc ttattgctac aatattggct gatccaaaac
      601 tctccacaat ctgggcattg ataataatca cctttataac caggctgctt catcataccg
      661 tttccttcag ttaggattga atgacataaa ggattccagc aaatcgacac caaacgagca
      721 tggtacatca agaagcagcg caacagactg tctaagcctt tctgatgact gggagtcaca
      781 caaactgcgc caattttatg cccaggccac cattctccgt ttgctctctc acggagtggg
      841 gtgggcgtaa atggctctcc cccaaaaaca gtatattggg ggtttggatc agagtgaagc
      901 acaaatgaag cgtcacattc aagcccattg agatacttca tttttgccgt tccaaatata
      961 tctgcctcaa atggcgtgtt aaccatatac ttcttgtctt tttcagacac atatttcata
     1021 tctgcgtaat ttctatactt tacatccaga gcaatttttt taatttgcgc cccagtctgc
     1081 agagtaaggg taatatcagg ttttaattct tcccaagtcc cagtattaat ttcgttgtaa
     1141 catggcagtg acggacaaat attctgatca aagcattttt tgcccaattt gcacggacgc
     1201 ggctttattt taggcgaata agacaagacc gccgtacaaa caggtacctt ggaaaaataa
     1261 ttctttttag gaacaaaact taacctatac tcactgcctt ctcgtaaatt tatatcttca
     1321 tcgtatactt caatggtgtc aaacggggaa tcgccgtcgg ggagaaatcc gaaatcatga
     1381 accaacctcg cataaacttc aaaaaaaacc cagatctcat acagtttaga gacgcgccgc
     1441 acggactttt cctccaaagc ccaggcaagg cttttttgcc gggaagattt tgcattactt
     1501 aaataatcct gatacgccct atagatcgga ccatatactt ttgaatccgc tatttttgct
     1561 gatgggcgaa acgaactaac attggaagta atattcctca aaaaaggttc ttgcaaatac
     1621 ccttctgcct tatttatgat tccttcgata tttgccgcct gactttcaag ccgattggct
     1681 tcctgttcaa tattaacacg acgttcctgc cacaaatgta aataattttg tccatgacgg
     1741 tagccgtctg gctttctttg aatttccttg atttttgcgg cttgcagcct gaaataattc
     1801 attaaaggct ttgccttggg aattaagata tctttcagaa catgaaccaa gaaacgattt
     1861 tcttctgaat cataggtttc ctgccgctct acaattagct gcctgcgttg gtgaggtttt
     1921 tgcacggatt gattcattga tcggatcgac tgtgctgact gtaagttagt caggtcaacg
     1981 atttttgtcc cgagtttgat ttctttggct ggattgttct tgatgttttc ccagttttcc
     2041 tcagttatct tggctaagtt ttcaaaggca tcaccactta attgtctttt c
//
LOCUS       DAOQJE010000703         2088 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_42341_length_2088_cov_5.408756, whole genome shotgun sequence.
ACCESSION   DAOQJE010000703 DAOQJE010000000
VERSION     DAOQJE010000703.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2088)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2088)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2088
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42341_length_2088_cov_5.408756"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..393)
                     /locus_tag="PLT08_15490"
     CDS             complement(<1..393)
                     /locus_tag="PLT08_15490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE05929.1"
                     /translation="MIRSVRSILMAFFLAVSVLVVQVSPVQALTYPAEINMSFTPISI
                     TSGGTSRLRVTIYNPNSNALTGAAWLDNLIGVQPGLSIAATPNVVQTCTGTVTDSGGG
                     AINPGDTSIRLTGGTVPGQVGSNPGSCYV"
     gene            721..>2088
                     /locus_tag="PLT08_15495"
     CDS             721..>2088
                     /locus_tag="PLT08_15495"
                     /inference="COORDINATES: protein motif:HMM:NF024583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase dimerization/phospho-acceptor
                     domain-containing protein"
                     /protein_id="HNE05930.1"
                     /translation="MSIVPSMLPDFRVRQRDYLLEIARLLTQELDLEKLMARILRVSV
                     EMLAGQAGIIALKLPDGWRVAAAHGIAPAFLSYLTPLLAEEKVRELDVTELNRMLKEL
                     TYTASMGLLNGTGLPLAAHGQVIGVIFIFRNYPDVFTPNDRILLQSFADQAAIAVFNA
                     QLYGQVTYEKKRLDALLDSAGDGILILDAELNIERCNEAFSKLYGSTREEIKDKPHKE
                     IIKWKKEPHGSTLEESIVNGWPLTPNATLYVEGDLSRPLPPTLPVGITYAPLLSDEGK
                     LRNVIVAVRDITHFRNADEMKATFISIVSHELRTPVALIKGYASTLRRADAKWDKNTI
                     NDSLTVIEEEADRLSKMIDDLLDASRLQAGGMSLNQADVALPSLALRVSERLASQSPK
                     HLIVTDFPEKFPIILGDENRLEQVISNLVSNALKYAPEGEIKIIGKVLPEQVVVCVSD
                     EGPGID"
BASE COUNT          594 a          533 c          464 g          497 t
ORIGIN      
        1 tacataacaa ctacctgggt tgctgccaac ctgtccgggg acagtccccc ccgtcagacg
       61 aatggaagta tcgccaggat ttatagcgcc gccgccactg tccgttactg taccagtaca
      121 tgtctgcacg acatttggtg tggctgcaat tgaaagtccg ggttgaacgc caattaaatt
      181 atccaaccag gcggcgcctg ttagggcatt cgaattcgga ttgtaaatgg tcacacgtaa
      241 acgcgaagtt ccaccagaag tgatggaaat cggtgtgaaa ctcatattga tttccgctgg
      301 ataggtcaac gcctgtacag gcgatacctg tacaaccagc accgatactg ccagaaaaaa
      361 cgccatcaaa attgaacgaa cagaacggat catggaaaac acctcaaaac aaaatacaaa
      421 ctcacagcgc ttagagataa tacactgtgt ctttaattaa caagcgaaga cggttcaccc
      481 gtcttcgcta taatgcatcg tacatctaca tggaaacaca gtcaaattca acagtagcgg
      541 gtaaaaaagt caattatcga ttagaaatcc ctaatcaaca ttaattgtat aggtaattag
      601 tagggtgtca acaccgagaa ttaacaaaaa agccaattca taatagtcac ttagacaact
      661 ttaggtgatt atatttctct tacacacatc cagaaatcgg gtgatacaat ccctgcaatt
      721 atgtccatcg ttccttccat gcttccagac tttcgtgtgc gccagcgaga ttacttgctg
      781 gaaattgcac gcctcttaac gcaagagttg gacttggaaa aattgatggc gcgtattttg
      841 cgcgtttcgg ttgaaatgtt ggctgggcag gcaggcatca tcgcgctcaa acttcccgac
      901 ggctggcgcg tcgctgctgc acatggcatc gctccagcat tcctctccta cctaaccccg
      961 ctccttgccg aagaaaaagt ccgcgagttg gatgtgaccg aactcaaccg catgttaaag
     1021 gaactgacct acactgccag tatggggtta ctcaacggaa ccggtcttcc tctcgcagca
     1081 catggacaag tcattggcgt tattttcatc ttccgcaatt atccagacgt attcaccccc
     1141 aacgatcgca tcctattaca atcttttgca gaccaagctg ccatcgccgt attcaacgcc
     1201 caactgtatg gacaggttac ctacgaaaag aaacgcctag atgctctgct cgactcggct
     1261 ggtgatggca ttctcattct tgatgccgaa ttgaatattg aacgttgtaa tgaagccttc
     1321 agcaaattgt atggttcaac tcgcgaagag atcaaagaca agcctcacaa agaaattatc
     1381 aagtggaaga aagaacctca cggcagcacg cttgaggagt ctatcgtaaa tggctggccg
     1441 ttaacaccca atgccacact ctatgtagaa ggcgacctca gccgtccttt gccgccaacc
     1501 ttgccagttg gaattaccta tgctccactg ctttcagatg aaggcaagct acgtaatgtg
     1561 attgtggcag tccgcgatat tacccatttc cgaaacgcag acgagatgaa ggcgaccttc
     1621 atctctattg tcagccatga actgcggacg ccggtagctc tcattaaggg atatgcctct
     1681 acattgcggc gggcagatgc aaaatgggat aagaacacca tcaacgactc actcaccgtc
     1741 attgaagaag aagcagaccg cctctcgaag atgatcgatg atctgctaga cgcctcgcgc
     1801 ttgcaagctg gaggcatgag cttgaatcaa gccgatgttg ccctacccag ccttgcgtta
     1861 agagtgagcg aacggttggc atcccaatca ccaaaacacc tcattgtcac cgacttccct
     1921 gaaaagtttc ctatcatcct cggcgatgaa aaccgattgg aacaagtcat ttcaaacctc
     1981 gtctcgaatg cactgaagta tgcacctgaa ggagagatca aaatcattgg caaggttttg
     2041 cccgagcagg tggttgtgtg cgttagtgat gaaggacccg gcattgac
//
LOCUS       DAOQJE010000704         2088 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_42369_length_2088_cov_2.393015, whole genome shotgun sequence.
ACCESSION   DAOQJE010000704 DAOQJE010000000
VERSION     DAOQJE010000704.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2088)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2088)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2088
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42369_length_2088_cov_2.393015"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(169..1104)
                     /locus_tag="PLT08_15500"
     CDS             complement(169..1104)
                     /locus_tag="PLT08_15500"
                     /inference="COORDINATES: protein motif:HMM:NF013348.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNE05931.1"
                     /translation="MKCKKCGEKASVNMRHHRMALCKDHFLEWIPEQTERFIKKYNMF
                     TRNEKILVAVSGGKDSLALWDILTRLGYNADGLYLGLGIDGGVQYSDESHHLTEEFAR
                     KNNLKLHVVDIEKEYGHSIPVLSEISFRGKGKPCSVCGLTKRHEMNRIARDLGYDVLA
                     TGHNLDDEAAVLFGNTLQWSPDYLLRQGPVLASKAGLARKVKPLCRFYEREMTAYSIA
                     RGIEYIYEECPFAEGATQIFYKETLNQLESTRPGIKLTFYLRFLEARQSGELFIQREE
                     TQPDIHPCEKCGQPTSAPGMCSFCRMLEKANVVTE"
     gene            complement(1108..1302)
                     /locus_tag="PLT08_15505"
     CDS             complement(1108..1302)
                     /locus_tag="PLT08_15505"
                     /inference="COORDINATES: protein motif:HMM:NF014636.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoaD/ThiS family protein"
                     /protein_id="HNE05932.1"
                     /translation="MPVKLVLRNKEFEVKAGMALLDALKKCNIMPESVIATRDGELIT
                     EDELLKDGEVIKLVAVISGG"
     gene            1438..>2088
                     /locus_tag="PLT08_15510"
     CDS             1438..>2088
                     /locus_tag="PLT08_15510"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNE05933.1"
                     /translation="MMVDSYKPYISGITNYVEVNKQYLEKAGHEVFVFTFGDLEYEDT
                     EPNVIRSPGLPLADTGFYLSMRYSRQAKKLLQTMDIVHVHHPFLSGRLALRYCRPAGI
                     PIVFTNHSRYDLYAQAYLPAMPEEVSVGMLQAYMPPFCKAVDLVITPSAGMARVLREL
                     KVESDVEIVPNGVDLTSFQNAHPLEREAFGYKPDDILIVYAGRIALEKNLPFLIESF"
BASE COUNT          546 a          534 c          434 g          574 t
ORIGIN      
        1 gcgcgatgcg ctgttcggaa agatcgaagt cccaattcaa gtggacgtgg agcgttacgg
       61 attcgaaacc ccgcgtttct ccgttttttc tacagagcag atcgcggggt atgtagcctt
      121 cggtttattt cttgtagggt tgcttgccac cgtcattcgg taggcagttt attctgtcac
      181 aacattcgcc ttttccaaca ttctacaaaa cgaacacatt cccggcgccg aagtcggctg
      241 accacatttt tcgcatgggt gaatatcggg ttgagtctct tcgcgttgaa tgaacaattc
      301 tccgctctgg cgcgcttcca gaaagcgcaa gtaaaacgtc aacttgatgc ctgggcgggt
      361 actctccaac tgatttaaag tctctttata aaaaatctga gtcgccccct ccgcaaatgg
      421 acattcctca taaatgtatt caataccacg agcaatggaa tatgccgtca tttcgcgttc
      481 gtaaaaacga cacaacggct tgaccttgcg agccaagccc gccttggatg caagtacagg
      541 accttgccga agtagataat caggagacca ctgtaaggta ttcccaaaca agacagccgc
      601 ttcatcatca aggttgtgtc ccgtcgcaag aacatcatag cccaagtctc gtgcgatacg
      661 gttcatctca tggcgcttgg tcagcccaca gacggaacaa ggcttacctt tgccacgaaa
      721 actaatctcc gaaagaaccg ggatggaatg tccatattcc ttttcaatat ccacgacatg
      781 caatttgaga ttgtttttgc gggcaaattc ttcggtcaaa tgatgtgact catcggaata
      841 ctgaacacca ccatcaatgc caagcccaag atacaaccca tctgcgttat aaccaagacg
      901 ggtcagaatg tcccacagtg ctagcgaatc cttgccacca gaaacagcaa caaggatctt
      961 ctcattgcga gtgaacatat tgtatttttt gataaaacgc tcggtctgct ctggaatcca
     1021 ctcaaggaaa tggtccttac acaacgccat gcgatgatgg cgcatattga ctgaggcttt
     1081 ttcaccacat tttttacact tcatcgttta gccgcctgag atcactgcaa ccaatttaat
     1141 cacttcccca tcttttagaa gttcatcttc tgtgatcaat tcgccatctc gagtggcgat
     1201 tactgattca ggcataatat tgcatttttt caatgcgtcc aacaaagcca ttccagcttt
     1261 gacttcaaat tctttatttc ttaaaaccag tttgaccggc atttttcgct cctgtcgctt
     1321 tacatagatt gacaatagac cttcgcaaca tttgtaaagg acggttattc taccatgcac
     1381 aaaagtagcc ttgacattcc tacctgcatg ccgtagcatc atattatgcg aattggaatg
     1441 atggttgaca gctacaaacc ctatatcagc ggcattacga attatgtcga ggttaataag
     1501 caatacctcg aaaaagccgg gcatgaagtg ttcgtcttta cctttggcga tcttgaatat
     1561 gaagacactg aacccaatgt tatccgaagc ccagggttgc cattggcaga caccggcttt
     1621 tatctctcca tgcggtattc acgtcaggca aaaaagctct tgcaaaccat ggacattgtg
     1681 catgtccatc accccttctt aagtgggcgt cttgccttgc ggtattgccg cccagctgga
     1741 attccgatcg tctttacgaa tcattcgcgc tacgacctct atgcgcaagc atacctccct
     1801 gctatgcctg aagaagttag cgttggaatg ttacaagcct atatgccgcc attttgcaaa
     1861 gctgttgatc ttgtcatcac accttcggca ggcatggcaa gagtgctacg cgaactcaaa
     1921 gtagaaagtg acgttgagat tgtccccaat ggtgttgacc tcactagctt tcaaaatgca
     1981 catccccttg agcgcgaagc ttttggctac aaacccgatg atattctgat cgtctatgcc
     2041 gggcgcattg cgttggaaaa gaatctaccc tttcttatcg agtccttt
//
LOCUS       DAOQJE010000705         2080 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_42683_length_2080_cov_3.782222, whole genome shotgun sequence.
ACCESSION   DAOQJE010000705 DAOQJE010000000
VERSION     DAOQJE010000705.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2080)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2080)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2080
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42683_length_2080_cov_3.782222"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(164..1711)
                     /locus_tag="PLT08_15515"
     CDS             complement(164..1711)
                     /locus_tag="PLT08_15515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE05934.1"
                     /translation="MKDRRYFLILIVLFVFACEMPSLTSRDIPIPAPTAIVTPTNADA
                     VTTPTNPIDINTNIIWLTFLEIINPGFGVKITLGKDGNIYVAKTIGVGDETASLMEFD
                     QNGEIKWETSFAKWEYGNYDDLEVDSKGNILLSGDSTTAWGNPINPYLENASTFNAFL
                     AKFDKNGSLLWNTFISDNSGGASISTDKEDNIYAVFVEFDNGISSGLSLVKFNSNGVQ
                     EWRKSYTSLVDATPLDLSVDSNGNILIGGSRGLGTGDFDCFASKFANNGDLLWNVYVG
                     IPESADSCDSTMDVDTNGNIYMAGVSTKSWGNPINPFVIHDRARDYNFFAAKLDGNGR
                     VVWNTFIDDFAHINDLSLDATGSFLYLVGSGESLINDESRKDGLIAQMDTDGRLLSKK
                     IIGDEGFDEIMYLAVGDDQRIYIIGDSSVTFGNPIHPITSDLSFTNLDTYLASISMKD
                     DEQNAESESLAPENNPPGNFLEQLTSGNVLRIILAALCCVGFALLAILAIVLTSRFFH
                     PGTRKQP"
BASE COUNT          573 a          477 c          446 g          584 t
ORIGIN      
        1 ttggatgcga tgctggtcgg gatgtttaaa aaataagacg ggctgcacgc ccgtcttatt
       61 ttttttcaac gtaactgctc atgctgcaag gcatggttgt agcatgaata gcatagtttt
      121 tcaaaactca atagattata agactgcgac ttattttcat gtctcatggt tgttttcggg
      181 tgccggggtg gaaaaacctt gaagtcaaaa cgattgccaa tattgcaagc agagcaaaac
      241 cgacgcaaca tagagcggca aggataatac gaagtacatt accgctcgtc aattgctcca
      301 gaaaattgcc aggtgggtta ttctccgggg caagagattc gctttcggcg ttctgttcat
      361 cgtccttcat cgaaatgctg gcgaggtatg tgtcaaggtt ggtgaacgag agatcgcttg
      421 ttataggatg tattgggttt ccaaaagtaa cggaactgtc gcctataatg tagatgcgct
      481 ggtcatcgcc aactgccaaa tacatgattt catcaaagcc ttcatctcct ataatttttt
      541 tagataacaa acgtccatcc gtgtccattt gagcgataag gccatctttt ctagattcgt
      601 cgtttatcaa agattcgccg gacccaacca aataaagaaa actgccggtt gcatctaatg
      661 acaaatcatt aatatgggca aagtcgtcta taaaagtgtt ccataccacc ctcccattac
      721 catctaattt cgcggcaaaa aaattgtagt ctctggctct atcatgaatt acaaacggat
      781 taatcgggtt tccccagctt ttggtgctca cccctgccat ataaatattg ccgtttgtgt
      841 caacatccat tgtggaatcg cagctatccg cgctttctgg aattccaacg taaacattcc
      901 aaagtaaatc accattgttc gcaaattttg atgcgaaaca atcgaaatct cccgtcccaa
      961 ggccgcgaga tcccccgata aggatgtttc catttgagtc cacacttaaa tcaaggggag
     1021 tagcatctac aagtgaggtg taagattttc gccactcctg aacgccattg ctattaaatt
     1081 ttacaagaga taagcccgaa gaaataccat tatcgaattc aacaaatacg gcatatatgt
     1141 tgtcctcttt gtccgttgaa atggaagccc caccgctgtt gtcgcttata aaagtattcc
     1201 acaacaaaga tccatttttg tcaaatttgg ctaaaaaagc gttaaatgtc gacgcgtttt
     1261 ccaagtaagg atttatcgga ttcccccatg ccgttgtact atcgcctgat aataaaatat
     1321 ttcctttgct atcaacttcg agatcgtcgt agtttccata ctcccatttg gcaaaggatg
     1381 tctcccattt tatttcgccg ttctgatcaa attccattaa cgaagccgtt tcgtccccaa
     1441 cgccgattgt tttggccaca tagatattcc catccttgcc tagcgttatt ttaacgccaa
     1501 agcccgggtt tattatttct aagaatgtca gccatatgat attggtattg atatctatcg
     1561 gattagtcgg cgtggtgaca gcgtcggcat tagtaggcgt aacgatggcg gtcggcgcag
     1621 gtatggggat gtctctgctt gttaaggatg gcatctcgca ggcgaaaaca aacagaacaa
     1681 ttaggataag gaagtagcgg cggtctttca ttgtgatatc ctattctacg ctttgctacc
     1741 atacctccgt cattgcgagg gcggtcttcc cgacacttgc accgcgccga ggaatttctt
     1801 aaagagatta ctagcagtta cttaaatcgt aacaggtcac atggtggcat cgaggaacgt
     1861 caaaataggc ggtctcatca aggcaaccaa tatgtaggcc atgtcgttcg gcagtattta
     1921 agacggttgc gagagtggca taaccccgtg cgccgcaatc agagcagaaa ctgcccctga
     1981 ccttccgatg aatcacggac ggacgcaagg cgcgttcaca gacactattc tacaaacctg
     2041 tacttgatca gggtccacac ggcttcaaag gcatcatgta
//
LOCUS       DAOQJE010000706         2077 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_42800_length_2077_cov_8.095945, whole genome shotgun sequence.
ACCESSION   DAOQJE010000706 DAOQJE010000000
VERSION     DAOQJE010000706.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2077)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2077)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2077
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42800_length_2077_cov_8.095945"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..245
                     /locus_tag="PLT08_15520"
     CDS             <1..245
                     /locus_tag="PLT08_15520"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024157.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNE05935.1"
                     /translation="EAATGAPTHGVIEVGVTTHENFRQKGLATIACAKLIEACETQGY
                     STWWDCAKQNTPSVRLAKKLGYQNEKEYRYAWWEKR"
     gene            434..1504
                     /locus_tag="PLT08_15525"
     CDS             434..1504
                     /locus_tag="PLT08_15525"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HNE05936.1"
                     /translation="MSRSHIPSVIQALIAALLFGASAPIAKLLLGEVEPIPLAAFLYL
                     GSGIGLLGIKLFQRINQQGVNDEAQVEKSDFKWLLGAIVAGGVAAPITLLFSLKNTPA
                     ATASLLLNFEGVATTLIAFFAFKESISRRAWWAIALITVASIFLSINFNAEWGFSLGA
                     LGIIAACVLWGVDNNFTRNISAKDPLVIVTIKGLGAGSFSLIMALFLGNNLPSWEVIL
                     GALVLGSLSYGASIVLFIHAMRGLGAARTSALFSTAPIAGIVLSILIFQEFPSWLFIF
                     ALPLMIVGAFLLVNDEHEHHHIHEMVIHEHSHSHNDGHHEHEHDGEFTKKHSHVHTHD
                     ELTHSHHHMPDLHHRHVHPSGS"
     gene            1571..2008
                     /locus_tag="PLT08_15530"
     CDS             1571..2008
                     /locus_tag="PLT08_15530"
                     /inference="COORDINATES: protein motif:HMM:NF021357.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromate resistance protein"
                     /protein_id="HNE05937.1"
                     /translation="MKWVTRSHVHVDRVACPWLITRFIDSEAELIYVAKNQVEQTVKE
                     TGAIPYDVPNVELGHKDGKCSFESIIEKYGLTDLALLRLAKIIHAADIESELETDPIA
                     AGLEAIATGFSIRFPDDLENLERQFEVYDALYAWCRLQVVKES"
BASE COUNT          559 a          432 c          483 g          603 t
ORIGIN      
        1 gtgaagcggc aactggcgcg cctacacatg gggtaattga agtcggagta accacacatg
       61 aaaattttcg gcaaaaaggt cttgcaacaa tagcgtgtgc aaaacttatt gaagcatgcg
      121 aaacccaagg ctattcaacg tggtgggatt gcgcgaagca gaatacgcca tctgtgcgcc
      181 ttgcaaaaaa acttggttat caaaacgaga aagaatatag atatgcatgg tgggagaaaa
      241 gatgaacgag tcttcctacc aacagcgccc aacaaagcgt gcacctgacg ctggggattc
      301 tgcggcaatc tcaagcagtt tcacacgcct tagccttttt ctggctggac ggcttcgccg
      361 tccccacccc agcgcaggta acgcaaaccg ttggcttgcc ccttgcataa cataataagg
      421 aaaatcatcg tttatgagtc gaagtcatat cccgtccgtt attcaagcct taattgccgc
      481 tcttttgttt ggcgcaagtg cgccgattgc gaaattgctt ttaggtgaag ttgagccgat
      541 tccgcttgcg gcatttttat atttgggtag cggtattggt ttgttgggaa taaaactttt
      601 tcagcgtata aatcagcaag gcgtaaacga tgaagcgcaa gttgaaaaat ccgatttcaa
      661 atggttatta ggggcgattg tagcgggcgg ggttgctgct ccaattacat tgctttttag
      721 tctaaaaaat acgcccgccg ccacagcctc tttacttttg aattttgaag gcgtagcaac
      781 aactcttatt gccttttttg ctttcaaaga atcaattagc cgccgtgcgt ggtgggcaat
      841 tgcattaatc acagtcgcaa gtatttttct ctcgataaat ttcaatgctg aatggggttt
      901 ttctcttggc gcattgggaa ttattgccgc ttgcgttctt tggggtgttg ataataattt
      961 cactcgtaat atttcagcaa aagatccttt ggttattgtc actatcaaag gtttgggtgc
     1021 agggagtttt tcgttaatca tggcgttgtt tttgggcaat aatcttcctt catgggaagt
     1081 tattttaggg gctttagttt tgggtagttt gagttacggc gcgagtattg ttttatttat
     1141 tcatgctatg cgtggtcttg gtgcggctcg cacaagcgca ttatttagca cagccccaat
     1201 tgcaggtata gttttgtcaa ttcttatatt ccaagaattt ccaagttggt tatttatctt
     1261 tgcgttgccg ttaatgattg ttggggcgtt tttattggta aatgatgagc atgaacatca
     1321 tcatattcat gaaatggtta ttcacgagca ttcacattct cacaatgacg gacatcacga
     1381 acatgaacat gatggagagt ttactaaaaa gcactcgcac gttcacacgc acgatgaact
     1441 aactcattct catcatcaca tgccagactt gcatcaccgt cacgttcatc cgtcagggtc
     1501 ataagaccat taggctttgt atggcgcatc cgcgcaataa atatcaattc aaattaaaag
     1561 aggaaatgct atgaaatggg ttactcgctc acacgtacac gttgaccgtg ttgcatgtcc
     1621 ctggcttatt acccgattca ttgactcaga agccgagtta atctatgttg caaagaatca
     1681 ggttgagcag acagtaaaag aaacaggagc gattccttat gacgttccca atgttgaact
     1741 tgggcataaa gacggaaaat gctcgtttga atccatcatc gagaaatacg gattgactga
     1801 ccttgctcta cttcggcttg ccaaaattat ccatgcggca gatattgaaa gcgaacttga
     1861 gacggaccca atcgccgcag gtctggaagc aatcgccact gggtttagca tccgtttccc
     1921 agatgacttg gaaaatctgg aaagacaatt tgaagtatat gacgctttgt acgcatggtg
     1981 tcgtttgcaa gtggtgaagg agtcttaaat tcaaaaagca agccaacaaa gcgtgcacct
     2041 gacgctgggg attctgcggc aatctcaagc agtttcc
//
LOCUS       DAOQJE010000707         2076 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_42847_length_2076_cov_4.866898, whole genome shotgun sequence.
ACCESSION   DAOQJE010000707 DAOQJE010000000
VERSION     DAOQJE010000707.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2076)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2076)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2076
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42847_length_2076_cov_4.866898"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(135..1160)
                     /locus_tag="PLT08_15535"
     CDS             complement(135..1160)
                     /locus_tag="PLT08_15535"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding response regulator"
                     /protein_id="HNE05938.1"
                     /translation="MNMRALIVDDDTSWQGILSEMLIDNGFEVDTASTVDEAVAALKD
                     NPHRLAVVDLSLSVSDHHNQDGLRVLDAVRLLDPNCRAILLTGFATVEVAVKALTEYN
                     AFTFLRKESFKRSEFKDVIQRILVSAPTVDEPHASSADLKLDSASQTSAPLKALIVDD
                     DAGWRSILEDLLSEAEFQPVPCASFGEALGLIRKDKFSLAIVDLSLRDTQSLDGLQLL
                     KITQGHHIPTIVVSGLSEPEEIQRIYAEHSISTYIEKQAFDRNAFKRILAETRELSTT
                     MDELSILTDREREVLDLLAQGLTNKEIAEKLVITTNTVKRHLKAVFEKLDVHTRAAAV
                     AIATKSK"
     gene            complement(1157..>2076)
                     /locus_tag="PLT08_15540"
     CDS             complement(1157..>2076)
                     /locus_tag="PLT08_15540"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HNE05939.1"
                     /translation="VPEKAPTVADDNALVMPLLSGEKLTALGVFGAFYAGTSGVRSGS
                     SEWDRKVLACLANYAVLAVQNEFRQQALRASQQQHLIAETFAAVGDISANLLHNMNNK
                     VGVIPVRVQGIQDKYAKLLGEEAYLAKSLSEIERSATAAMQIVQENLSHLRPIRLERI
                     QVAPRVNDAIHAVSAPADIHIEVDGLENLPTVNASGQSLVFVFRNLIENAIDAMNGKG
                     EIKIRGEQNGSWVEVSVSDTGRGIPPEIHEQIFELAYSSRMHPGKMGFGLWWVKTLMT
                     RLGGSVTVESDGASGTTFYLRIPIAEQVS"
BASE COUNT          499 a          501 c          527 g          549 t
ORIGIN      
        1 gaaaggctat tgtcagggag atggatatcg tattggcttt ggcgaagtga ccgggaagat
       61 tcttccggca aagtaaaccc tttaactgcg aagctcgcca agtttttctt gacgagcttc
      121 gcagttgata gtccttattt actttttgtt gcaatggcta ctgcagcagc acgggtgtgg
      181 acatctagct tttcaaacac ggctttcaag tggcgtttta cagtgttggt tgtgatgacc
      241 agtttttcag caatctcttt gttggtcaac ccttgagcga gcagatctag cacttcgcgt
      301 tcgcgatctg tgaggatgct tagctcgtcc atggtggtgc tcagttcgcg ggtttcggcg
      361 agaatgcgtt tgaaagcatt tcggtcaaag gcttgttttt cgatgtaagt agaaatcgaa
      421 tgttcggcgt aaatccgctg aatttcttca ggttcggaaa gcccactgac aacgatagtg
      481 ggaatatgat gcccttgggt aatcttgagc aattgcagcc cgtcgaggct ttgcgtatcg
      541 cgcagggaaa ggtctacaat ggcaagtgag aatttatctt tgcggatcaa ccctagcgct
      601 tcgccaaagc tggcacaagg cacgggctga aattctgctt cactgagcag gtcttccaga
      661 atactgcgcc aaccggcatc atcatccacg atcaacgctt tcaggggcgc agaggtttga
      721 gaagcagaat ccagtttcaa gtccgcagaa gaagcgtgtg gttcatccac ggtgggggcg
      781 ctgaccaaaa tccgctgaat gacatccttg aactcactgc gcttgaaact ttctttgcgt
      841 agaaaggtaa aggcgttgta ttcggttagt gcttttacag ccacttcaac cgtggcaaag
      901 cctgtaagta gaatggcgcg acaatttggg tcgagcaaac gcacggcatc cagcacgcgc
      961 aaaccatctt gattgtgatg gtcgcttaca gagagggaaa ggtctacaac cgcaaggcga
     1021 tgaggattgt ctttgagcgc cgcgacagct tcatctactg tgctggcggt gtcgacttca
     1081 aagccattgt caatgagcat ctcgctcaaa ataccttgcc aggaagtatc gtcatcgacg
     1141 attaatgcac gcatgttcat gatacttgct ccgcaatggg gattcgtaaa taaaaggttg
     1201 tgccgctagc gccatcgctc tcaacggtga cagatccgcc aaggcgtgtc atcagagttt
     1261 tgacccacca caaaccaaaa cccattttgc cggggtgcat acggctggaa tatgccagct
     1321 caaaaatttg ttcgtgaatt tcaggtggaa tgccgcgacc tgtatcgctc acagacactt
     1381 ctacccaact gccattttgt tcgccgcgaa tttttatttc gcctttgccg ttcatggcgt
     1441 cgatggcatt ttcaattaag ttccgaaata cgaacacaag gctttgtccg ctggcgttaa
     1501 cggtgggaag attttccaac ccgtctactt caatgtgtat atcggctggc gcagaaaccg
     1561 catgaatggc gtcgttcaca cgcggtgcga cttgaatgcg ttccaaacga atggggcgca
     1621 agtgcgaaag attttcctga acgatttgca tggcagccgt agcactacgt tcgatctcgc
     1681 ttaaactctt ggcgagatag gcttcctcgc ccaacagctt ggcatactta tcttggatgc
     1741 cttgcacgcg cacaggaata acacccactt tgttattcat gttatgtaag agattcgcag
     1801 aaatatcacc gacagccgca aaagtttctg cgatgagatg ttgttgctgc gaagcacgta
     1861 atgcttgttg tctgaattcg ttttggacgg caagcacggc atagttggcg agacatgcca
     1921 acactttccg gtcccattca gaggaaccgc ttcggactcc gctcgtgcct gcatagaacg
     1981 ccccaaacac tcccaaagct gtgagctttt ctccgcttag caagggcata accaatgcat
     2041 tgtcatctgc tacagttggt gctttttctg gcacag
//
LOCUS       DAOQJE010000708         2074 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_42934_length_2074_cov_8.274889, whole genome shotgun sequence.
ACCESSION   DAOQJE010000708 DAOQJE010000000
VERSION     DAOQJE010000708.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2074)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2074)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2074
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42934_length_2074_cov_8.274889"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..218)
                     /locus_tag="PLT08_15545"
     CDS             complement(<1..218)
                     /locus_tag="PLT08_15545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNE05940.1"
                     /translation="MTTVFDSTKTVKTELGKQNYIASDEIATIVYLAQKLGKPLLAEG
                     PAGVGKTELAKAIASATGRQLIRLQCYEG"
     gene            complement(202..525)
                     /locus_tag="PLT08_15550"
     CDS             complement(202..525)
                     /locus_tag="PLT08_15550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE05941.1"
                     /translation="MAYYLVTAKPIASKIKDLRTWLDSGEIHAMRPFGNALQTGLDNA
                     RWLSDGVATWEEEDYCVPPLAQERAAVLDAYFTNIEVEHVTKGEGWKKIEPLKSIWET
                     HDNRI"
     gene            complement(741..1250)
                     /locus_tag="PLT08_15555"
     CDS             complement(741..1250)
                     /locus_tag="PLT08_15555"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024917.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HNE05942.1"
                     /translation="MDIIIRAEQASDIEAITQVTYAAFLGKFSDHPTEHLIVNGLREA
                     GMLTLSLVAEVNGKLVGHVAFSPVTINEKFVDWYGLGPISVAPELQKQGIGSKLITDG
                     LARLRNLGAKGCVLEGSPAYYSRFGFKPRAGLTYHAAPAPEYFMALPFYEEAPAGNVE
                     YHQAFYVSP"
     gene            complement(1253..>2074)
                     /locus_tag="PLT08_15560"
     CDS             complement(1253..>2074)
                     /locus_tag="PLT08_15560"
                     /inference="COORDINATES: protein motif:HMM:NF012432.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lyase family protein"
                     /protein_id="HNE05943.1"
                     /translation="MTLGQEFSTYAVMLEEDQQRLREGALLIQEMNLGATAIGTGINA
                     HPEYASLARKHLSEITGIEYITAGNLVEATQDVGSFVQLSGVLKRVAVKLSKICNDLR
                     LLSSGPRTGFGEINLPAVQAGSSIMPGKVNPVIPEVVNQIAFLVIGNDVTVSFAADGG
                     QLQLNAFEPIIAHSLFDSLIYLRNGCITLAERCVKGITANEERLNEHMTRSVGIVTAL
                     NPFIGYENASSVAKEAYTSNKSVTEIVLERGLLTKAELDEILKPEVLTKPRWIKK"
BASE COUNT          463 a          549 c          518 g          544 t
ORIGIN      
        1 ccttcatagc attgcaagcg gatgagttgc cgtcccgtcg cggaggcgat ggctttggca
       61 agttcggtct tgcccacgcc cgcaggacct tccgccagca gcggcttgcc taacttctgg
      121 gcgaggtaca caatggttgc gatttcgtct gaagcaatgt agttctgttt gcccagctcg
      181 gtctttacgg ttttggttga atcaaatacg gttgtcatgg gtctcccaaa tagatttcaa
      241 tggttcgatc ttcttccagc cttccccctt ggtcacgtgt tcaacttcga tgttcgtgaa
      301 atatgcatcc agcacggcag cgcgttcctg agccagaggc ggcacgcaat agtcctcttc
      361 ctcccaggtg gcaacgccgt ctgagagcca gcgcgcgtta tccagtccgg tttgaagcgc
      421 attcccgaac ggacgcatgg catggatctc ccccgaatcc agccatgttc gcaagtcttt
      481 tatttttgac gcgataggtt tggcagtgac aagatagtat gccatctgct cctccttctg
      541 atgatgtgat tctctcacac ttttggggtt gtcaggcgct tctatcgctt gtctcataat
      601 taagtaaact tttcgttgaa aattacgagg gaaaatcgaa atttttgttt actaaattaa
      661 gcgatactct gtaagaattg cctaacatcc gctgattgca tatagacgtg accgcttgcg
      721 aattcctgac tttcctaatc ttacgggctg acataaaaag cctgatgata ttctacgttg
      781 cctgcgggcg cttcttcata aaaaggaagc gccatgaaat attcaggtgc aggcgcggca
      841 tggtaggtga gaccagctct cggcttgaat ccgaagcgcg aataatacgc tggactgcct
      901 tcgagcacac atccttttgc gccgaggttg cgtagtcttg ccaacccatc cgtaattaac
      961 tttgagccaa tgccttgttt ctgcaactca ggtgcaaccg agatcggacc caatccatac
     1021 caatccacaa acttctcgtt gatggtcaca ggcgagaacg ctacatgccc gacaagtttt
     1081 ccattcactt cagcgacgag tgaaagcgtg agcatgcccg cctcacgcag accgttcacg
     1141 atgaggtgct cggtggggtg gtcgctgaac ttgccaagaa aagcggcata cgtcacctgc
     1201 gtgatggctt cgatatcgct tgcctgctca gcccgaatga tgatgtccat gtttattttt
     1261 tgatccaacg cggcttggtc aacacttcag gtttgaggat ttcatccagc tctgctttgg
     1321 ttagcagacc acgctccagc acgatctcgg tcacgctctt attggatgtg tacgcctctt
     1381 tcgccaccga gcttgcgttc tcgtacccga tgaacggatt caacgctgtg acgattccga
     1441 ccgagcgggt catgtgctca ttcaatcgtt cttcattggc agtgatgccc ttcacgcagc
     1501 gttcagctaa ggtgatgcat ccattgcgca gataaatgag actgtcaaac agactgtgcg
     1561 cgatgatcgg ctcaaaagca ttgagttgca actgtccgcc atcggcagca aaggagacgg
     1621 tgacatcgtt tccgatgaca aggaaagcga tctgattgac cacttcaggg ataactgggt
     1681 tgaccttgcc tggcatgatg cttgaacccg cctgaacggc aggcaggttg atctcgccga
     1741 atccagttcg tggacccgat gaaagcaaac gcaggtcgtt gcagatcttc gagagtttga
     1801 ccgccacacg tttgagcacg cctgaaagct gcacgaacga accgacatct tgagtcgctt
     1861 caacaagatt tcccgcggtg atgtattcaa tgcctgtgat ctcactgagg tgtttccgcg
     1921 ccaatgatgc atactcagga tgcgcattga tacctgtgcc aatggctgtc gcgccgaggt
     1981 tcatttcttg aatgagcaaa gcaccttcgc gcaaacgttg ctggtcttct tcgagcatca
     2041 cggcatacgt gctgaattcc tgcccaaggg tcat
//
LOCUS       DAOQJE010000709         2072 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_43006_length_2072_cov_5.016857, whole genome shotgun sequence.
ACCESSION   DAOQJE010000709 DAOQJE010000000
VERSION     DAOQJE010000709.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2072)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2072)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2072
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43006_length_2072_cov_5.016857"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..476)
                     /locus_tag="PLT08_15565"
     CDS             complement(<1..476)
                     /locus_tag="PLT08_15565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047429 - nucleoside triphosphate
                     diphosphatase activity [Evidence IEA];
                     GO_process: GO:0009143 - nucleoside triphosphate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="non-canonical purine NTP pyrophosphatase"
                     /protein_id="HNE05944.1"
                     /translation="MSKLLIATNNQGKVRELQELLTGSGIEFVTPAQINLQIDVEEDG
                     STYQENAGKKAIAFAKASGLISLADDSGLEVEALGGAPGLYSARYSPKPGANDKDRRD
                     FLLSNLQDKPRPWKAHFHATIAVANPTGEVEFTDGNCYGEIIPEERGSGGFGYDPI"
     gene            complement(473..1519)
                     /gene="queG"
                     /locus_tag="PLT08_15570"
     CDS             complement(473..1519)
                     /gene="queG"
                     /locus_tag="PLT08_15570"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0052693 - epoxyqueuosine reductase
                     activity [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HNE05945.1"
                     /translation="MFNNSITLKQAIKEKARQLGFFLAGVTSSASLAHFDIFQEWLDA
                     NHHASMDYLASDRSRLRRADPKQILPECKSVLVLALPYSPISKNSSEFQIASYALGDD
                     YHEVIPPRLQEIVQFIEQQVGHPIPNRYYTDTGPVLERELAQRAGLGWIGKNSMLIHP
                     QKGSTFLLAEILLGIELEPDEPFQTDHCGTCTRCMDACPTQCILPNRTIDSNRCISYL
                     TIENKDDIPEALRPRIQNWVFGCDICQQVCPWNRFSLPTDPNLEPSIPLPVLTSDLLL
                     TSSEFNQRFKRSPIKRTKRRGYLRNLAVAVGNQKNKKDLPLLHQATQDEETLVQSHAQ
                     WAIETINRNNTGNL"
     gene            complement(1509..>2072)
                     /locus_tag="PLT08_15575"
     CDS             complement(1509..>2072)
                     /locus_tag="PLT08_15575"
                     /inference="COORDINATES: protein motif:HMM:NF027484.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NBR1-Ig-like domain-containing protein"
                     /protein_id="HNE05946.1"
                     /translation="PIEPTPDVNAVRTSAASTVIAEFTLTAAFFTPTPLPLPTETPTP
                     FPTETPAVLPSIDPTLAALTPDALCDDYDFDPATIDVTIPDGTPMTPGQDFVKTWKIK
                     NTGICTWDTSYKAIFSYSNPPNQQMSAQPIPLAALVAPGQEVDVSVQFTAPTTPGEYL
                     GVWQMVNAKGIPFGQPGKQLTVKIVVQ"
BASE COUNT          515 a          434 c          549 g          574 t
ORIGIN      
        1 aagatagggt catatccaaa cccgccacta ccacgctctt cagggatgat ctcaccatag
       61 caatttccat cagtaaattc aacttcacca gtagggttgg caaccgcaat agtggcatga
      121 aagtgggctt tccatgggcg tggcttgtct tggagattgc tcaaaagaaa atcccgccgg
      181 tctttatcat ttgcaccagg tttcggggaa taccgtgcgg aatataagcc aggcgcgcca
      241 cctaatgctt ccacttccag ccctgagtca tctgcgaggg agatcaagcc gctggctttg
      301 gcaaatgcga tggctttctt gccggcattt tcttgatagg tggaaccgtc ttcctcaaca
      361 tcgatttgaa ggttaatctg tgcaggagtc acgaattcaa tacccgaacc cgttaataat
      421 tcttgaagtt cgcgtacttt cccttggtta ttggtggcaa taagaagttt actcataaat
      481 ttcctgtgtt atttctattt atagtttcaa ttgcccattg ggcatgagat tgcacgagag
      541 tttcttcatc ttgagtagct tgatgcaaga gaggtaagtc ttttttattt ttttgatttc
      601 caacagcaac agcaaggttt cgcaaatatc cacgtcgctt ggtgcgtttg attggagacc
      661 gtttgaagcg ttgattaaac tcggaggatg tcagaagcag gtcagaggtc aagacaggaa
      721 gcggaatgga tggttcgagg ttagggtctg ttggcaggga gaatcgattc cacgggcaga
      781 cttgctgaca gatgtcgcat ccaaaaaccc aattttgtat tcgaggtcgc aaggcttcag
      841 gaatatcatc tttgttttcg atggtgagat aggagataca tcgatttgaa tcaatcgtgc
      901 gatttggcaa gatacattgg gttgggcagg catccatgca acgggtgcat gtgccacaat
      961 gatcagtttg aaaaggctca tccggttcga gttcgatacc aagcaagatt tctgcaagta
     1021 gaaaagtaga acctttctgc ggatgaatga gcatggaatt cttcccgatc cacccgagcc
     1081 cggctcgctg cgccaattcg cgttctaaga cgggacccgt atctgtgtaa tagcgatttg
     1141 gaataggatg cccaacttgt tgctcaataa attgtacgat ctcttgcaaa cgtggtggaa
     1201 tgacttcgtg gtagtcatct ccaagtgcat atgatgcgat ttgaaattca gaactatttt
     1261 ttgagattgg ggagtatggc agtgcaagaa ctaaaacaga tttacattcg ggcaaaattt
     1321 gtttcgggtc tgcgcggcgt aaccgactgc ggtctgaggc gaggtaatcc atgctggcat
     1381 gatgattcgc atccagccat tcttgaaaaa tatcaaaatg tgcaagtgat gcagaagaag
     1441 taacgccagc taggaaaaat cccagctggc gcgctttttc ttttatagct tgctttagtg
     1501 tgattgaatt attgaacaac gatctttaca gtgagttgct tgcccggctg tccaaatgga
     1561 atgcctttcg cattcaccat ttgccagacg cccaagtatt cacctggggt ggtaggcgct
     1621 gtaaactgaa cagatacatc tacttcttga ccaggagcaa ccagtgccgc aagcggaatg
     1681 ggctgagcgc tcatctgttg attgggcggg ttgctatatg agaagatcgc tttgtaactt
     1741 gtatcccaag tacagatgcc tgtatttttg atcttccatg tttttacaaa atcttgcccg
     1801 ggtgtcatgg gcgtcccatc gggaatggtc acatcgatgg tggctgggtc aaaatcataa
     1861 tcatcgcaga gagcatctgg ggtcaacgcc gccaatgttg ggtcgatcga aggaagaacc
     1921 gccggagttt ctgttgggaa gggcgttggg gtttcagtgg gcagcgggag tggtgtaggc
     1981 gtgaagaaag ctgcggtcaa tgtgaattcc gcgatcactg tgctggctgc ggatgtgcga
     2041 actgcgttta cgtcgggcgt tggctcgata gg
//
LOCUS       DAOQJE010000710         2068 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_43172_length_2068_cov_5.164928, whole genome shotgun sequence.
ACCESSION   DAOQJE010000710 DAOQJE010000000
VERSION     DAOQJE010000710.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2068)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2068)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2068
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43172_length_2068_cov_5.164928"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            124..525
                     /locus_tag="PLT08_15580"
     CDS             124..525
                     /locus_tag="PLT08_15580"
                     /inference="COORDINATES: protein motif:HMM:NF019013.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030638 - polyketide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ester cyclase"
                     /protein_id="HNE05947.1"
                     /translation="MSVETTKEVLTRYLNSGHSDQSMLAEDVSFTIMATGEEHRGREG
                     VSAMLNYFYHIAFEADAETRTLVFGETSAIWEGVIVGKHIGEYASVPATGKNVRVPIC
                     VVYDVEDKRVKRARVYFEVPAFYQQVGVKMG"
     gene            748..1110
                     /locus_tag="PLT08_15585"
     CDS             748..1110
                     /locus_tag="PLT08_15585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE05948.1"
                     /translation="MKALRNTLIALAVYMTVVGALFLFAPSVAEKAFSTSLPDRALTP
                     LYGELMIVIALMAYLVSTDVSAYKKLVIAFIVSEIGHVLIFGYQLMTGIATFAQVGPP
                     MVIAIIFTVLLFVFKGKA"
     gene            1122..1340
                     /locus_tag="PLT08_15590"
     CDS             1122..1340
                     /locus_tag="PLT08_15590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE05949.1"
                     /translation="MESDEAGARTKSLLFTLRLWSADENTHAPQWRVRLQNVHTGEVH
                     YCGDEKSLNTLLDDILSEVLKEKRKEER"
     gene            1349..1858
                     /locus_tag="PLT08_15595"
     CDS             1349..1858
                     /locus_tag="PLT08_15595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE05950.1"
                     /translation="MLLLLLLILSLILSACGSSPAPTHAPTSGGFDIKPFLGVYEGTW
                     TNKTTGASGPAVIIVEAKEADKTVTLTLDFDGNYLGLNDPPAFPLTAKYDDNGAYVRG
                     ESEILGKMDITIDTQGNIVGNLSDLLGGTIPSMTYTGKIGNGHLDTDYIVTLSDGTVT
                     VSELRTVRK"
     gene            1865..>2068
                     /locus_tag="PLT08_15600"
     CDS             1865..>2068
                     /locus_tag="PLT08_15600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE05951.1"
                     /translation="MKKLLPLLATFMFLFQACGGSASAPAATEAPANAASTEAAVTEA
                     PTAEPIVHTTIPQAGTVSRANAHD"
BASE COUNT          510 a          551 c          509 g          498 t
ORIGIN      
        1 ctttgctaaa gcaagccgtg gcttgggtaa aggaacatca ttacctgtaa cgttccaatg
       61 aaacggtttt gaaacggctt ctccgtacac tgggggcaac tcaacaactt aaggagaagc
      121 accatgtctg tcgaaactac aaaagaagta ctgacacgct acctcaactc tggtcacagt
      181 gaccagagca tgctggcaga ggatgtcagc tttaccatca tggcaaccgg tgaggaacac
      241 cgtggtcgag aaggtgtttc ggcgatgctg aattatttct atcacatcgc ttttgaagca
      301 gatgccgaga cgcgcaccct ggtcttcggc gaaacgagcg ccatttggga aggtgtcatt
      361 gtggggaagc acattggcga atatgcaagc gtacctgcca caggaaagaa tgtccgagtg
      421 ccgatctgtg tggtgtatga cgtggaggat aagagagtca agcgggctcg ggtctatttt
      481 gaagtccctg ctttttatca gcaggtcggc gtcaaaatgg gataacctcc cctcttaatg
      541 caaagtcgct aagccgccat gtttcaaatt cctttctttg cgaccctgcg cctttgcgtt
      601 aaaaatatac aaggtgaaaa aagctatgat ccccgccttc taggcgggga ttttctttcc
      661 cgagaaacgc tccatgaaac gcttttgaaa cgccccatcc ataaaatgag cacagttcga
      721 tatttcaact catttacgga ggctcttatg aaagcgttac gaaataccct tattgcattg
      781 gctgtctaca tgacggtcgt cggagctttg ttcctctttg ctcccagcgt ggcggaaaag
      841 gcattcagta catccctgcc agaccgcgcc ctcaccccgc tctacggcga attgatgatc
      901 gtcatcgccc tgatggcata tctcgtctcc accgatgtct ccgcctacaa gaaattggtc
      961 attgcattca tcgtttcgga aattgggcat gtgctcatct tcggctacca gctaatgacc
     1021 ggcattgcca ccttcgccca ggtcggtccg cccatggtca ttgccatcat cttcaccgtc
     1081 ctgctctttg tcttcaaggg caaagcctaa aaggttgtga tatggaaagc gatgaggctg
     1141 gcgctcgcac aaaatcgctc ctgtttacct tgcgtctttg gagtgccgat gagaacacgc
     1201 acgcgccgca atggcgcgtg cgtctccaaa atgtacacac gggcgaggtt cattattgtg
     1261 gggatgagaa gtcgctgaac acattgctcg atgatatttt gagcgaagtt ctcaaggaaa
     1321 aaagaaaaga ggaacggtga ttagaaaaat gttattactt cttttgctta ttttgtcttt
     1381 gatattgtca gcctgcggtt cttcccccgc tccaactcac gctccaactt caggcggatt
     1441 cgacatcaag ccgtttctcg gcgtctacga aggcacttgg accaacaaga ccactggcgc
     1501 gagcggaccg gcagtcatca tagttgaagc gaaagaagcc gataagacag tgaccctcac
     1561 gcttgatttt gacggcaact atcttgggtt gaacgacccg ccagccttcc cgctcacagc
     1621 caagtatgac gacaatggtg catacgtccg cggcgagagc gaaatccttg gcaagatgga
     1681 catcaccatt gatacccaag gcaatatcgt gggcaacttg tccgatttat tgggcggaac
     1741 gatcccctca atgacctaca ccggcaagat cggtaatggt catctcgata ccgattacat
     1801 cgtgacttta tccgatggta cggtgaccgt ctccgaactg cgcaccgttc gaaagtgagc
     1861 tgaaatgaaa aaacttttac ccttgctggc aacctttatg ttcctgtttc aagcctgtgg
     1921 cggaagcgcc agcgctccag ccgccactga agctccggcg aatgccgcat ccacagaagc
     1981 ggctgtgact gaagcgccga ctgcagagcc catcgtccac accaccattc cccaggcagg
     2041 gacggtctcc cgtgccaatg cacatgac
//
LOCUS       DAOQJE010000711         2066 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_43259_length_2066_cov_6.063650, whole genome shotgun sequence.
ACCESSION   DAOQJE010000711 DAOQJE010000000
VERSION     DAOQJE010000711.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2066)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2066)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2066
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43259_length_2066_cov_6.063650"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..455
                     /locus_tag="PLT08_15605"
     CDS             <1..455
                     /locus_tag="PLT08_15605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE05952.1"
                     /translation="VAWHILRALAARMGLPAPDSYEDDFPEDSQVDFAFYLQLTPEMA
                     EDIAQYEYVCFVDAHTGNIPEEVRIIDVESDFQKSPFTHHLTAQSLMSICETVYHRKP
                     DAALLSVRGYQFLFSRLLSTPTAALVPEAVDLAWAWINAREPLKQAGD"
     gene            593..1039
                     /locus_tag="PLT08_15610"
     CDS             593..1039
                     /locus_tag="PLT08_15610"
                     /inference="COORDINATES: protein motif:HMM:NF012780.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HNE05953.1"
                     /translation="MTTVRKLLEAKEFATNYSVETNQTVLDALKVMAEAHIGAVLVTD
                     GGKIVGIYTERDYLYKGEALGRVAKDTKIKDVMTPKMINVTMETTVEQCMGLMKQYKI
                     RHLPVVEGEHFVGLVSMRDVMLAAIDIKESEIRGLENYIMGSGFQS"
     gene            1117..>2066
                     /locus_tag="PLT08_15615"
     CDS             1117..>2066
                     /locus_tag="PLT08_15615"
                     /inference="COORDINATES: protein motif:HMM:NF013630.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HNE05954.1"
                     /translation="MSFRKTVSVFLLAILIVSCAPATEEPATFPTYNPFVSTNGATLP
                     PNSNVAVTTPLSSETVIPSATSTRPPTPTRVPLNISSLIGVQSQIISGSPTPDTARVL
                     PTPRQDADQYVVQAGDTLGAIAQRYNITLQTLMDANGITDPNALEVGMTLNIPAAQVT
                     VSSGQSLKIIPDSELVNGPATAKFSLRNFIASQNGYLNYYTQDVNGTLLNGADIIEAV
                     SYNYSVNPRILLALLEYQSGWVTNPTPTNIDYPMGYYDEARAGLYRQVVWAANTLNRG
                     YYLWKANGLSTWVLTDGQVLPIDPTINAGTAAVQYFFSQL"
BASE COUNT          550 a          506 c          469 g          541 t
ORIGIN      
        1 gtgttgcctg gcacatcctg cgggcgttgg cagctcgaat ggggttgccc gcgcctgatt
       61 cgtatgaaga tgatttccct gaagactcgc aagtggattt cgccttctac ttgcagctca
      121 cgcccgaaat ggcggaagat attgctcaat atgaatacgt ctgctttgtg gacgcgcaca
      181 cgggcaatat tccagaggaa gtacgcatca ttgatgtaga aagcgacttt cagaaatcac
      241 cattcacgca tcacctcacc gcccagtcgt tgatgtccat ttgcgaaaca gtctatcatc
      301 gcaaaccaga tgcagcattg ctctcggtgc gcggatatca atttctgttc tcgcgcctcc
      361 tttccacgcc gacagctgct cttgtgcccg aagctgtgga cttagcttgg gcgtggataa
      421 atgcccgaga accgctgaaa caggctggag attaaggatg agccgaaagc aaagagtgca
      481 gacgggtcaa aaataaatca gatgacaaac gtcacgcgag atgaggaggt tttccgtgtg
      541 acgttttatt ttgcctatga taaaaaagaa cttatataag gagacgaaaa tcatgaccac
      601 tgtccgaaaa cttctggaag ctaaggaatt tgcgacaaac tattctgttg aaaccaatca
      661 gacggtattg gatgccctca aggtgatggc tgaagcccat attggagctg tgttggtaac
      721 agatggcgga aagatcgttg gaatttatac tgaacgtgat tatctctaca aaggggaagc
      781 gttggggcgg gttgccaaag atacaaagat caaagatgtg atgacaccga aaatgatcaa
      841 cgtaaccatg gaaactactg ttgagcaatg catggggctg atgaaacagt ataagatccg
      901 ccacttacct gtagtggaag gcgaacattt tgtaggtttg gtttctatgc gtgatgtgat
      961 gctggctgcg attgacatca aagaaagcga aattcgcggg cttgaaaatt acatcatggg
     1021 gtctggtttt cagtcctagt tctctcaaca aacttgaaaa gaggcgcaat gtttgcgcct
     1081 ctttttttac cgatacgatt tggtaaaatc acttttatgt cttttcgcaa aactgtatct
     1141 gtgtttttac ttgctattct catcgtttct tgcgcacctg ccacagaaga acctgcaact
     1201 ttccctacct ataacccttt cgtttctaca aatggagcaa cccttccgcc aaatagcaac
     1261 gtggcggtaa ccacgcctct gtcttctgaa acggttattc cgtcagcaac ctccacgcgc
     1321 ccgcccacgc ctactcgtgt gcctttgaac atttcttctc tcattggtgt acaaagtcag
     1381 attatctccg gctcacctac tccagatacg gctcgggtgt tgcccactcc gcgccaagat
     1441 gcggatcaat atgtcgtcca ggctggtgat accttaggtg ctatcgctca acgttataac
     1501 atcacccttc aaactcttat ggatgccaat ggcatcaccg acccaaatgc attagaagtt
     1561 ggcatgacat taaacatccc agccgctcaa gttactgtat caagcggaca atccctcaaa
     1621 attattccag actccgaact ggtcaacgga ccggctacgg caaaattcag tttacgcaat
     1681 ttcattgcca gccagaatgg ctacctgaat tactacaccc aagacgtgaa tggaacttta
     1741 ttaaacggcg cagatattat tgaagccgtg tcttataact attccgtcaa cccgcgcatt
     1801 ttacttgcct tgcttgaata tcaaagcggc tgggttacca acccaacacc aaccaatatc
     1861 gattacccca tggggtatta cgacgaagcc cgcgccgggt tatatcggca ggtggtttgg
     1921 gcggcaaaca cactaaaccg cggttattat ctctggaaag ccaatgggct ttctacttgg
     1981 gtgttaacag atggtcaggt gcttcccatt gatcctacaa tcaacgctgg cacagccgct
     2041 gtacaatatt tcttctcaca gcttta
//
LOCUS       DAOQJE010000712         2065 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_43292_length_2065_cov_4.529353, whole genome shotgun sequence.
ACCESSION   DAOQJE010000712 DAOQJE010000000
VERSION     DAOQJE010000712.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2065)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2065)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2065
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43292_length_2065_cov_4.529353"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..723
                     /locus_tag="PLT08_15620"
     CDS             <1..723
                     /locus_tag="PLT08_15620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE05955.1"
                     /translation="TDNTRQHYYPSLSPDSKSVVYAAFREPNIYEIYEMDIATGNVAQ
                     LTDRLGNLNGPEISPDGSMIAFKLSTAAANQIWLMNRDGSDPHPISNAFGWDPTWYPD
                     GTSILFASDMQGVVQLYVIKLDGSELQKISDLPAIRGRSDWSPNGQFIVTYSGQPWNR
                     DVYIMNADGSDARMLTPTGGNSQGPSISPDGQWVVFTSYYDHYGEDHGCEIYIMPVTG
                     TEQVVRRLTDNDYCDYQPRWGP"
     gene            740..1567
                     /locus_tag="PLT08_15625"
     CDS             740..1567
                     /locus_tag="PLT08_15625"
                     /inference="COORDINATES: protein motif:HMM:NF024950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HNE05956.1"
                     /translation="MDIKGKVVIITGASSGIGAAAALEFGREGAKVVLAARRTDKLQE
                     LVNEIRAMQNGAEAFVLQTDISKLDDIQRLVAQTVERFGRVDVLVNNAGFGRLKWLET
                     LDPLEDIQAQFDVNVLGVIQTTRQVLPIMIKQRSGSIINICSIAGLVGTPTYTIYSAT
                     KHAVHGFSEALRREVKPWGIDVSLIYPGGVRGTEFPDHAGIQRKTNASTPKFMVLTAE
                     QVGKAVVSLVHRPRRMWVLPWMWRFTIFLNRFLPGLVDRTTIKNFTIPERADELNKK"
     gene            complement(1591..>2065)
                     /locus_tag="PLT08_15630"
     CDS             complement(1591..>2065)
                     /locus_tag="PLT08_15630"
                     /inference="COORDINATES: protein motif:HMM:NF037628.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE05957.1"
                     /translation="LVRHGGHAAAAGFTVKNENIEALVSKLKAIAEERLAGSDLKPTV
                     TADAEVDLSEMRPEVFEKGLKYLEPTGYGNREASFVARNVKVKSSRIVGSDGKHIKMS
                     LEDAKGVVHDAIGFRLGDWHKSMPPRVDVLFTYEPNEYNGRVTYQLNLKDLKASR"
BASE COUNT          528 a          504 c          501 g          532 t
ORIGIN      
        1 accgataaca ctcgtcaaca ttattaccca tcgctatcgc cagatagcaa aagtgttgtt
       61 tatgcagcat ttcgtgaacc gaatatttac gaaatctacg aaatggatat tgccactggc
      121 aacgttgctc aactaaccga ccgtctcggc aatctcaacg gaccggaaat ttcgcctgat
      181 ggttcaatga tcgcgttcaa actctccacc gcggcagcca atcagatttg gttgatgaac
      241 cgtgatggga gcgacccgca tcccatttcc aatgcttttg gctgggaccc aacttggtat
      301 ccagatggaa cctccatttt gtttgcctct gatatgcaag gtgtcgttca gctctatgtg
      361 ataaagctcg atggaagcga actgcaaaag atcagcgacc tgcccgcgat acggggaaga
      421 agcgattggt caccgaatgg acagttcatc gtcacatatt cgggtcagcc ttggaatcgc
      481 gatgtttaca tcatgaatgc agatggttcg gatgcccgca tgctcacacc aacgggcggt
      541 aactcgcaag gtccatccat ttcaccagat gggcagtggg tggtgtttac ctcttattat
      601 gaccattatg gcgaggacca tggatgtgag atctacatca tgcctgtgac tggcacagag
      661 caagttgtac gccgactaac agacaacgat tattgtgatt accaaccccg ctggggacct
      721 taagaacaac aaggacaaca tggacatcaa aggaaaagta gtaatcatca ccggcgcttc
      781 gtcggggatt ggggcagcgg ctgcgcttga atttgggcgt gaaggggcga aggttgtgtt
      841 agctgcacgg cgaactgaca agttacaaga attggtgaat gagattcgtg caatgcagaa
      901 tggcgcagaa gcctttgtgc ttcaaaccga tatctcaaaa ctggacgaca ttcaacgttt
      961 ggttgctcaa accgtagaga gatttggtcg tgtggatgtg ttggtgaata atgcaggctt
     1021 tggacgtttg aaatggctcg aaacgctcga cccacttgaa gatattcaag cacaatttga
     1081 cgtgaatgtt ttgggcgtta ttcaaaccac acgacaagtt ttgccgatca tgatcaaaca
     1141 gcgcagtggg agtattatca acatctgctc cattgctggt ttggtgggca cgccgaccta
     1201 tacaatttac tctgctacca aacatgccgt gcatggcttt tcagaagctt tgcggcgcga
     1261 agtcaaacct tggggaattg acgtctctct catctacccc ggcggcgtgc gtggaacaga
     1321 attccccgac catgccggta ttcagcgcaa gaccaacgct tccacgccaa agttcatggt
     1381 attaacggcg gaacaagtag gcaaagctgt tgtgagcctt gtacaccgcc cgcgccggat
     1441 gtgggtgtta ccgtggatgt ggcgatttac tatttttctc aatcgctttt tgccagggct
     1501 tgtagaccgc actacgatta aaaacttcac cattccagaa cgagcggatg aactcaataa
     1561 aaaataaaca gccgcaaggc tgtttatttt ttatcttgag gctttcaagt ctttcaaatt
     1621 caattggtat gtaacgcgtc cgttgtattc atttggctcg taagtaaaaa gcacatccac
     1681 tcgcggcggc atggacttat gccaatcgcc gagccggaac ccgatcgcat catgcacaac
     1741 tcccttagca tcttcaagtg acatcttgat gtgcttgcca tcagaaccta caatgcgcga
     1801 acttttgact ttgacattgc gtgctacaaa cgaagcttcg cgatttccat acccggttgg
     1861 ttcgagatat ttcaagcctt tctcgaaaac ttcaggacgc atttcggata agtccacttc
     1921 cgcatctgcc gtaacggtgg gcttgaggtc acttccagcg agtctttctt cagcgatggc
     1981 ttttagctta ctaacaagcg cttcaatgtt ttcatttttt acggtgaaac ccgctgcggc
     2041 ggcatgcccg ccatgacgca caagc
//
LOCUS       DAOQJE010000713         2062 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_43418_length_2062_cov_5.933234, whole genome shotgun sequence.
ACCESSION   DAOQJE010000713 DAOQJE010000000
VERSION     DAOQJE010000713.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2062)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2062)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2062
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43418_length_2062_cov_5.933234"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..949
                     /locus_tag="PLT08_15635"
     CDS             <1..949
                     /locus_tag="PLT08_15635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE05958.1"
                     /translation="FMISIMLGSVLFVLGLLEIFIRDHRTRVFVLSALIALGIGQQFF
                     NANIFRRDWQRQGEIYWQMAWRMPTLAENTVLLTHQMPIDYETDLSFTAPINWMYAPN
                     YTRANLPYMLLYTEKRLGGSTLPSLDKSTSITYPYRTVQFDSTLSNAIVIYMPQNGCL
                     RVMDPSRGHAEIYDNIADEFTQAIAISDPSRILVKSTPPAEPMFFPEPAKDWCYYFAM
                     VELYNQWNEYQVSAEFADEALAAKFQPEDPREWIVFIEAYAMNGELDKAVEASNSALK
                     MDKKTLKAVCQTWEQVQAKGPEGSAEGFESARLSLGCNP"
     gene            1017..1781
                     /locus_tag="PLT08_15640"
     CDS             1017..1781
                     /locus_tag="PLT08_15640"
                     /inference="COORDINATES: protein motif:HMM:NF013630.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HNE05959.1"
                     /translation="MSNKRRSVLALSIFMVTAVMLSACTQSLSSAPAETPTLLPTGLF
                     VSPIASVENPMEMIEQFAKQTEAAQTSAAGGGTPGTPGTPQAVSTITTGTPGTPAPAV
                     ASVTPSAPTVSVSTPTNANAAAPTAAVPTSSSNSAATAPSLSGNTYVLQDGEWPYCIA
                     RRYNVDPDAMLQASGLTSPDIYYSGLKLVLPQNTTFPGARVLIAHPASYTVASGDETV
                     YSIACKYGDVDPNALASANGISVSSKLTVGQVLSIP"
     gene            1855..>2062
                     /locus_tag="PLT08_15645"
     CDS             1855..>2062
                     /locus_tag="PLT08_15645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE05960.1"
                     /translation="MPSFELIRSEPLMQGRAFKIRRDYLKTPDGHETKYDIVEHGGSV
                     VIVPFDGDGNLLFVRQYRHAAGMDM"
BASE COUNT          509 a          574 c          461 g          518 t
ORIGIN      
        1 ttttatgatc tccatcatgc tcggttcggt cttattcgtg ttaggtttgc tagagatatt
       61 catcagagac catcgcacac gtgtctttgt cctttctgcc ctcatcgccc tcggaatcgg
      121 acagcaattc ttcaatgcca acatctttcg cagagactgg caaaggcaag gtgaaatcta
      181 ctggcaaatg gcatggcgca tgcccacatt ggcagagaat accgttctcc tcacccatca
      241 aatgcccatc gattacgaaa ccgacctctc cttcaccgca cccatcaact ggatgtatgc
      301 gccgaactat acccgtgcca atcttcctta tatgttgctt tacacagaaa agcgtttggg
      361 cggttctacc ttaccgtcat tagataaatc tacatccatc acctatccat atcgaactgt
      421 acaatttgat agcacactct cgaatgccat agtgatttat atgccgcaga atggctgtct
      481 acgcgtcatg gacccaagcc gtggtcacgc cgaaatttac gacaacatcg ccgatgaatt
      541 cacccaagcc attgcaatct cagacccatc gcgcatcttg gtcaagtcca cgccgccagc
      601 cgagccgatg ttcttccctg agccagccaa agactggtgc tattacttcg ccatggtgga
      661 actttacaac caatggaacg aataccaagt ctctgccgag tttgcagatg aagcgctggc
      721 agccaagttc cagcctgaag acccacgcga gtggatcgtc ttcattgaag cgtatgccat
      781 gaacggcgaa ttggataaag cagttgaagc ctccaactct gctcttaaga tggataagaa
      841 aacgctcaaa gcggtctgcc aaacctggga gcaggtgcag gcaaagggtc cagaaggaag
      901 cgcagagggg tttgaatccg ctcgattgtc acttggttgc aatccatagc gctggcttga
      961 cgctttaaac ttcatgtgct accatctttt tttagtaaat agaacgagga gaatctatgt
     1021 ctaataaacg ccgcagtgtg ctggcattgt cgatctttat ggtcacagcg gtaatgcttt
     1081 ctgcctgcac acaatcactt tcgtctgctc ccgcagaaac accaactctc ttacctacag
     1141 ggttgtttgt ttcaccgatc gcttctgttg aaaacccaat ggaaatgatc gagcagttcg
     1201 ccaagcagac cgaagctgct caaacatccg ccgctggcgg tggcaccccg ggtacccccg
     1261 gaaccccaca agcagtttct acaatcacca caggcacccc cggaacaccc gcaccagccg
     1321 tcgcttctgt taccccttcg gctcctacgg tgagtgtttc cacccccacc aatgcaaatg
     1381 cagcggctcc tactgccgca gtgccaactt cttcatcgaa ctctgctgca acagcaccca
     1441 gcctttctgg caacacctat gttctgcaag atggtgaatg gccttactgt attgcccgtc
     1501 gttacaacgt ggaccctgac gccatgcttc aggcaagtgg tcttaccagc ccagatattt
     1561 actattctgg tttgaagttg gtccttccgc agaataccac cttccccggt gctcgcgttt
     1621 tgatcgcgca cccggcttcg tacacagtcg cttcgggtga tgaaacagtg tacagcatcg
     1681 cttgtaaata tggtgatgta gacccgaatg cactcgcgag tgcgaatgga atttctgtct
     1741 cttccaagtt gacggttggg caggttctga gcattccgta atttcaaaaa gacaaaagcc
     1801 caggtcaaca gcctgggctt tttttataga aactattctt gaaaggaaat cactatgcca
     1861 tcatttgaac tcattcgctc tgaacctctc atgcagggac gtgccttcaa gattcgccgt
     1921 gattatttga aaacaccaga tggtcacgaa accaaatacg atattgtaga acatggcggc
     1981 tcggtggtga tcgtgccatt tgacggggat gggaacctgc tcttcgtgcg acaataccgt
     2041 cacgcggctg gtatggatat gc
//
LOCUS       DAOQJE010000714         2060 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_43524_length_2060_cov_3.070823, whole genome shotgun sequence.
ACCESSION   DAOQJE010000714 DAOQJE010000000
VERSION     DAOQJE010000714.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2060)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2060)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2060
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43524_length_2060_cov_3.070823"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..193
                     /locus_tag="PLT08_15650"
     CDS             <1..193
                     /locus_tag="PLT08_15650"
                     /inference="COORDINATES: protein motif:HMM:NF014671.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="formate dehydrogenase accessory
                     sulfurtransferase FdhD"
                     /protein_id="HNE05961.1"
                     /translation="YELLQKSFSAGISVFCAVSAPSSLAVEFAERFGITLIGFLRGQR
                     FNVYANVQRIQVVEPVFSN"
     regulatory      240..337
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00162"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="SAM riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF00162"
     gene            384..1562
                     /locus_tag="PLT08_15655"
     CDS             384..1562
                     /locus_tag="PLT08_15655"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021134.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 87 protein"
                     /protein_id="HNE05962.1"
                     /translation="MKIIENKTRFILVFVLAIAILGAFFLPIPSHMDAQILYVADLGF
                     VKGVSPYDLQGHVDLIAALTGKAPENILVQPFPYPPWYVLSTFYLGLLPQDIAARIWF
                     EINFAMTILAIWFLTDGWKPKWQLTAMVAALLFMPVIGSMYIGQYNFTILLGAAMLVY
                     SLKKENVPLTTIAFALLTFKPHLGVFLAIVMFIYLIFRRDEFSKRSIKAILLAGAILF
                     VSGFIVDFPWPVHYFESLTRFRGIVECDSCASLPVLLMHLATGEDTLELGFLIGVITY
                     PLLFLIVYLVRRDIFHSPDLLISIAVPIVLMSYPYLFSYDYVLFLIPLLILFGTVSRI
                     DWFWVLPLYFLPTVLTFIFVRDVSRYLPLLALSVFVLQLIRARGEPSKFVHVETAPRL
                     "
     gene            1583..2059
                     /locus_tag="PLT08_15660"
     CDS             1583..2059
                     /locus_tag="PLT08_15660"
                     /inference="COORDINATES: protein motif:HMM:NF017015.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HNE05963.1"
                     /translation="MKLDLIKLYIEYHIETTRRVWKLIDTITDEQFIADEIYSHGSIR
                     NLMVHLASVDRRWLAGLKNLEDVGHLKQEDYATRAAAREVFENVAKDLSEHVAALTED
                     VLSMPTDKVEQPQWQILLHLVNHGTDHRATVLQQLAELGVKTFPQDLVIWLWERKK"
BASE COUNT          509 a          473 c          459 g          619 t
ORIGIN      
        1 ttatgagctc ttgcaaaaaa gtttttcggc gggcatctcg gtcttctgtg ccgtatccgc
       61 gccgagcagt ttggcagtgg aattcgccga acgctttggc attaccttga tcggcttcct
      121 acgcggacag cgctttaacg tctatgccaa tgtgcagagg attcaagttg ttgaacccgt
      181 attttcaaat tgacaaaact ttctccgtta tgatataaac ttaaaataag ttagttccgt
      241 actcatccag agaggctgag ggaccgaccc tttgaagcct cggcaaccat tcgttcggtg
      301 ccaatttcgg caggtgaata atcctgggag atgagagagg atatgcaact tcatgtcgcc
      361 tctcacttcg agaggcattt tttatgaaaa taattgaaaa caaaacgagg ttcatcctgg
      421 tatttgtcct ggcaattgca atactcggag ctttctttct acccatcccc tcccacatgg
      481 atgctcaaat cctatatgtt gccgatctgg gatttgtcaa aggggtttcg ccctatgacc
      541 tgcaaggtca tgtcgatttg atcgcagcgc ttaccggcaa ggccccggaa aacattttag
      601 ttcaaccatt tccatatcca ccctggtatg tgctttccac gttctatttg ggattgttac
      661 cccaggacat cgccgcacgc atctggtttg agatcaactt tgccatgacc attcttgcca
      721 tctggttcct tacggatggc tggaaaccca aatggcagct gaccgccatg gtagctgcgc
      781 ttctattcat gccggtgatc ggcagcatgt atatcggaca atataacttc accatcctgt
      841 taggggcggc aatgctggtt tattctctaa aaaaagaaaa tgttcctttg accaccatcg
      901 catttgcatt gctgaccttt aagccgcatc ttggagtctt cctggcgatt gtcatgttta
      961 tctacctgat cttccgccgt gatgagtttt cgaaacgttc gatcaaggct attctcttgg
     1021 caggcgcgat tctatttgtt agcggtttca ttgtggattt tccatggccg gttcactatt
     1081 tcgaatcatt aacccgcttt cgaggtatcg tagaatgcga ttcgtgcgca agtttgccgg
     1141 ttttattgat gcatctagcc actggtgaag acaccttgga actgggcttt ttgatcggag
     1201 tgattactta cccactgctg ttccttattg tatatcttgt gagaagggat atttttcact
     1261 ccccagattt attgatatcc atagcggtgc caatcgtact tatgtcatat ccatacctct
     1321 ttagttatga ctatgtactt ttcttaatac cattactaat cctgtttgga accgtctcaa
     1381 gaattgactg gttctgggta ctaccgctat attttctccc cactgtattg acctttatat
     1441 ttgttcgtga tgtcagtaga tatctgccgt tattggcgtt gtccgtattt gtactgcaat
     1501 taattcgagc aagaggagaa ccttcaaaat ttgtgcatgt agaaactgcc ccgcgacttt
     1561 aaaggtaaaa ccggaaagaa atatgaaact cgatttgatc aaactgtata tagaatatca
     1621 tatcgaaaca acccgccgtg tatggaagtt gatcgacacc attaccgacg agcaatttat
     1681 cgcggatgag atctactcac acggttccat ccgcaatttg atggtacacc tcgccagtgt
     1741 agaccgccgc tggctggcag gattgaagaa cttggaagat gtcggtcact tgaaacagga
     1801 agattatgca acccgtgctg ctgcgcggga agtatttgaa aacgtagcaa aggatttatc
     1861 agagcatgtt gctgcactta ctgaagatgt actgagcatg cccacagaca aggtcgagca
     1921 gccgcaatgg caaattcttt tgcatctcgt taatcatggc acggatcatc gtgcgactgt
     1981 cttacagcag ctcgctgaac ttggcgtaaa gacctttcca caagatttgg tcatctggtt
     2041 gtgggaaagg aagaaataaa
//
LOCUS       DAOQJE010000715         2058 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_43603_length_2058_cov_3.069895, whole genome shotgun sequence.
ACCESSION   DAOQJE010000715 DAOQJE010000000
VERSION     DAOQJE010000715.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2058)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2058)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2058
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43603_length_2058_cov_3.069895"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..651)
                     /locus_tag="PLT08_15665"
     CDS             complement(<1..651)
                     /locus_tag="PLT08_15665"
                     /inference="COORDINATES: protein motif:HMM:NF012804.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HNE05964.1"
                     /translation="MGLFDSEKKLIVETAHELVHKGFLMATGGNLSLRVANKAAFAVT
                     PSNYDYMKMTPEDVCILDFDLNMLEGHLKPSVESAMHGAVYQARGDVNAIIHTHQVYT
                     SALTLIKSPIPALFDEQARFLGRSVEIIPYAPSGTGMLKNTVAKHVKNNNNAFMMQNH
                     GALVFGHDMERAVHNVEILEKCAMAYLLAICTEKKISKIPLAVREVAFAKLRKDQKK"
     gene            735..1334
                     /locus_tag="PLT08_15670"
     CDS             735..1334
                     /locus_tag="PLT08_15670"
                     /inference="COORDINATES: protein motif:HMM:NF024701.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HNE05965.1"
                     /translation="MPPKKYHHGDLKNALIKAGVEILAKEGVDGLSLRKVAQHAGVSH
                     SAPYSHFSDKQSLIAAISTEGFNQLYAELEAAVSPYSKNPKKQLIEGAKAYVRFAENN
                     TDTFKIMFSGVLEKEKDYPSFVEISSKTFKLVVEVIQACQNAGILPTAPPELMAVSVW
                     GQIHGIVSLALEGQVSHTVLDRHSTQEIILFSLEQRLNS"
     gene            1497..1985
                     /locus_tag="PLT08_15675"
     CDS             1497..1985
                     /locus_tag="PLT08_15675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE05966.1"
                     /translation="MESIEYGLLLAGAIFCAYRATVSKRILSATLYLACISALTSIVI
                     FMLGAKQVAVMELSVGAGLVTVLMVYAVSVVGEDALDPKSVIAKPLAFILIGLVTLLI
                     GWMVYPAVHVSPSTGVTDLASVLWQNRVLDVWIQIVLIFSGVMGILGLLSERGPVKEA
                     QK"
BASE COUNT          528 a          502 c          459 g          569 t
ORIGIN      
        1 tttcttctgg tctttgcgca gcttggcaaa agcaacttcc ctcactgcca atgggatttt
       61 gctgatcttc ttttcagtgc agatcgccaa tagatacgcc atggcacatt tctctagaat
      121 ctcaacattg tggacagctc gttccatatc atgaccaaag accagtgccc catgattttg
      181 catcatgaac gcattgttat tatttttgac atgctttgcc acagtattct tcagcatccc
      241 cgtccctgac ggagcatacg gaatgatctc cacactgcgc cccagaaaac gcgcctgctc
      301 atcgaacaga gccgggatcg gtgacttgat cagagtcaac gcgctggtgt acacctgatg
      361 cgtgtggatg atggcgttca catctcccct tgcctgatac actgccccat gcatcgcgga
      421 ctcgaccgag ggcttgagat gtccttcaag catgttcaag tcaaaatcca aaatgcaaac
      481 gtcttccggc gtcatcttca tataatcata gtttgacgga gtgacggcaa aagccgcctt
      541 atttgctaca cgcaaagaca aatttccacc cgtcgccatt aagaaaccct tatgcactaa
      601 ttcgtgcgcg gtttcaacga tcaacttttt ttcagagtca aaaagtccca tagaataatc
      661 cttctcaact agacactgtc cagattttag aacacaatct tgacggtgtc aagattttcg
      721 tttaacatcg tttcatgcca cctaaaaagt accaccacgg agatctaaag aatgccctta
      781 ttaaagccgg agtagagatt ctggcaaaag aaggcgtaga cggattaagt ctgcgcaaag
      841 tggcacagca tgccggcgta agtcactctg ccccctattc acatttttct gataagcaat
      901 ccctcattgc ggcaatctcc actgaagggt ttaatcaact ctatgccgag ttagaagcgg
      961 cagtctctcc ctactccaag aatcctaaaa agcagctcat cgagggcgcg aaagcatatg
     1021 tccgctttgc ggaaaacaat accgacacct ttaagatcat gttctccggt gtgttggaaa
     1081 aagaaaaaga ttacccatcc tttgtagaga tatcaagtaa gacgtttaaa ctggttgtag
     1141 aagtcataca agcctgccaa aatgctggca tcttacccac cgctcctcca gagctaatgg
     1201 ctgtttccgt ttggggacag atccatggca tcgtatctct tgctctggaa ggacaggtat
     1261 ctcacactgt attagaccgt cattctactc aggagattat tttgttctcg cttgagcaaa
     1321 gattaaactc ctaattccag gtaattcaca caagcttcat ctcaattaaa gatttatttg
     1381 aagttttcca tagcgcttta tttgtgttat atttcacaat ataagtaatg accccaaagg
     1441 aggcgagagg tatgtggcag cgaatgccca tacagaagta attggagttg ttcaacatgg
     1501 aaagcatcga atatggctta ttgctcgcgg gggctatctt ttgtgcgtat cgtgcaacag
     1561 tttctaaacg cattctctcg gcaactttat atttggcgtg catcagcgca ttgacgtcca
     1621 tcgttatttt catgctaggc gcgaaacaag tcgcagtgat ggagctatcc gtcggcgcag
     1681 gcttggtgac agttctgatg gtgtatgccg ttagcgttgt cggtgaagat gcgttggatc
     1741 ctaaatcagt gatcgcaaag ccattggcat tcatcctgat tggtttggtc actctcctca
     1801 tcggttggat ggtgtacccc gctgtccatg tcagcccaag tacaggcgtc acagacttgg
     1861 caagcgtcct atggcaaaac cgcgtgcttg atgtgtggat ccagatcgtg ctgatctttt
     1921 ccggcgtgat gggaattttg ggattgctat cagaacgagg tccggtaaag gaggcgcaga
     1981 aatgaacctc tcccctctca acgttatttt gatcggaatc gccgggctgc tcggcgtcgg
     2041 cttttacggt ctattgat
//
LOCUS       DAOQJE010000716         2058 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_43609_length_2058_cov_2.756865, whole genome shotgun sequence.
ACCESSION   DAOQJE010000716 DAOQJE010000000
VERSION     DAOQJE010000716.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2058)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2058)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2058
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43609_length_2058_cov_2.756865"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..823)
                     /locus_tag="PLT08_15680"
     CDS             complement(<1..823)
                     /locus_tag="PLT08_15680"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNE05967.1"
                     /translation="MKIALITNSRIPSLTANSIQAMKVAQALMQLGHDIKIFAPKETD
                     VVNHETLLTHYGLRLTPDLELLPSISRLKRLDFIVHAQRAAKKFGAELIYTWLPQSAA
                     LGSWMGYPVVLEMHADNSGKLGVWWMRQFWNQKTPKVMTVTTSALRKALERSTKFQFK
                     DEALLLAPNGVELEKYAGLPSPTEARRQLNLKEGLTVGFTGHIYPGRGADLLFELAKQ
                     MPAVNFLWVGGTPELVEFWRGKLNEAGMTNVTMTGFVKHEVIPLYQAAADVLLMPY"
     gene            complement(801..>2058)
                     /locus_tag="PLT08_15685"
     CDS             complement(801..>2058)
                     /locus_tag="PLT08_15685"
                     /inference="COORDINATES: protein motif:HMM:NF024832.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HNE05968.1"
                     /translation="NNTISMGLSVVQSIMATRLLGPAGYGLIGLVMAFPATINSIFSF
                     RMNEVIVRYGGDYLNKQEKEKAAALLKAAGLTEAVVSLLAFLVVLFTASLAETYSAKT
                     PGTAWMFTVFAIGLLANFNTETSTGILQITGRISLLGVINLIQSIFTLLIITGAFFLN
                     GTLVTVLIAYLVGKTILGLGMFFTAQAQIHKALGSGWWRHSFSNLDSWREIFRFSVSS
                     NISATIIKIFRESEVLWISFFLDTTAVGYYRVAYNLVYFLAIPADPLIHVTFPEISRL
                     VTEKAWGKLKSFLKKITTLSLAYNIFLGAAYIFLGRWILLIFSGEKFVNAYPALVALT
                     IGLVFNYILFWNRPLLLTLGLPEFPIYITLVSGFIKLALSFWLVPTYGILAAGALLSY
                     YYITSVGIMALRGVRQIQHNENSSHH"
BASE COUNT          590 a          495 c          491 g          482 t
ORIGIN      
        1 agtacggcat tagcaacaca tccgccgcgg cttgatacag gggaatgact tcatgcttca
       61 cgaagccggt catcgtcacg ttggtcatac ccgcctcatt caatttgcct cgccaaaact
      121 ccaccaactc gggcgtgcct cccacccaca aaaaatttac cgcgggcatt tgtttggcga
      181 gttcaaataa taagtccgca cctctgccag gataaatatg ccccgtgaat ccaaccgtca
      241 gcccctcttt caaatttaat tgacgacgtg cctcggttgg acttggcaaa cccgcatact
      301 tctccaactc gacaccattc ggcgcgagca gcagcgcttc atccttaaac tgaaacttgg
      361 tcgagcgctc caaagccttt ctcaacgctg acgtggtgac cgtcatcacc ttgggagtct
      421 tctgattcca aaactgtctc atccaccaca cgccaagttt gccggaatta tctgcatgca
      481 tttcgagcac cacaggataa cccatccacg aacccaacgc cgcggattga gggagccaag
      541 tgtaaattaa ttcagctcca aacttcttcg cagctcgctg agcatggacg ataaagtcga
      601 ggcgtttgag tctgctgatg gatgggagca gttcaaggtc gggtgtgagg cgcagaccgt
      661 aatgcgtgag gagagtctcg tgattcacta catccgtttc ttttggggcg aatatcttga
      721 tgtcgtgccc taattgcatg agagcctgag cgaccttcat cgcttgaatc gagttggcag
      781 ttagagatgg gatgcgtgaa ttagtgatga gagctatttt cattgtgttg aatctgcctc
      841 actccgcgca gagccatgat accaacggag gtgatgtaat agtacgaaag taaagcgcct
      901 gcagcaagga tgccatatgt tggcaccagc caaaacgaaa gcgcaagttt gataaaccct
      961 gagacaagcg taatataaat gggaaattct ggcagcccca gcgttagcaa tagcggacga
     1021 ttccaaaaga ggatgtaatt gaacacaaga ccaatggtca gcgcgacaag tgcggggtag
     1081 gcattgacaa atttttcacc ggaaaagata agaaggatcc agcgtcctaa aaagatataa
     1141 gcagcgccga gaaaaatgtt ataagccaat gaaagcgtgg tgatcttttt gaggaaactt
     1201 ttgagtttgc cccatgcttt ttctgttacc aaacggctaa tctctgggaa ggtcacatga
     1261 ataagcgggt cggcagggat ggcaaggaaa tacacaaggt tgtatgctac gcgataatat
     1321 ccaacggctg tggtatcgag aaaaaagcta atccaaagca cttcgctctc gcggaagatc
     1381 ttgataatgg tagcgctaat gttggaacta acagagaagc gaaatatctc acgccatgaa
     1441 tcaagatttg aaaaagagtg ccgccaccat ccactgccca aggctttatg aatttgcgct
     1501 tgtgctgtga aaaacatacc caaccccagg atggtcttgc cgactaaata agcaatcagc
     1561 actgtaacaa gcgtgccatt tagaaagaaa gcgcctgtga tgataagcaa agtaaaaatg
     1621 ctttgaatga ggttaatgac ccctaataga ctaattcgcc cagtgatctg aagaatgcct
     1681 gtagatgttt ctgtgttgaa atttgccaat aacccaatgg caaatacagt gaacatccat
     1741 gccgtgccag gcgtcttggc ggaataagtt tcagcaagac tggctgtgaa aagcaccacc
     1801 aaaaatgcca gcagtgaaac aacggcttcg gttaatccag cggctttgag caaagcggcg
     1861 gctttttcct tttcctgctt attcaggtaa tctccaccat agcggacgat gacttcattc
     1921 atgcgaaatg aaaaaatact attgatcgtt gcaggaaacg ccatgaccag cccaatgaga
     1981 ccgtacccgg caggtcccag taagcgggtt gccatgatgc tctgcaccac acttaatccc
     2041 atagagatgg tgttgttg
//
LOCUS       DAOQJE010000717         2051 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_43850_length_2051_cov_6.259018, whole genome shotgun sequence.
ACCESSION   DAOQJE010000717 DAOQJE010000000
VERSION     DAOQJE010000717.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2051)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2051)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2051
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43850_length_2051_cov_6.259018"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            71..610
                     /locus_tag="PLT08_15690"
     CDS             71..610
                     /locus_tag="PLT08_15690"
                     /inference="COORDINATES: protein motif:HMM:NF015690.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF309 domain-containing protein"
                     /protein_id="HNE05969.1"
                     /translation="MPKEQEINSIERTAEECQGKLHPKAIVGLQLFNQGEFFEAHEEL
                     ETAWRDETSSIRDLYRGILQIAVTYLHITRGNYDGAVKVYGRSLKWLQGWGDICRGVN
                     IKKLRRDAETVMQEVTRLGRERIREFDRSLFQPIQWNEKRLWLCDRCGSEMYEKNCKV
                     SCPTCGNRFDCSDLNLYFD"
     gene            626..1393
                     /gene="fabL"
                     /locus_tag="PLT08_15695"
     CDS             626..1393
                     /gene="fabL"
                     /locus_tag="PLT08_15695"
                     /EC_number="1.3.1.104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-[acyl-carrier-protein] reductase FabL"
                     /protein_id="HNE05970.1"
                     /translation="MNFQNKIALVTGSGRGIGKAIALNFASQGADIVVNFFRNRAPAE
                     ETVREIEKLGRRALLVKADVGDLDDLNRLFDEVEKEFGGLDIFIHNAASGYNRPAMEQ
                     KPKGWDWTMNINARALLFAAQRATPLMEKRGGGSIVSISSAGSVRVLPDYVVVGASKA
                     ALEALTRYLGVELIQRGIKVNAVSPGVVETEALHHFSSMGGQENILQKFIERVPAGRL
                     ITPEDVAEVVAFLCSPASSMIVGQTIVVDGGYTLPIQ"
     gene            1405..>2051
                     /locus_tag="PLT08_15700"
     CDS             1405..>2051
                     /locus_tag="PLT08_15700"
                     /inference="COORDINATES: protein motif:HMM:NF014244.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HNE05971.1"
                     /translation="MFQAAPPTRFDLQFSIAGIPVRVHPLFWVIAILLGSGSSNLLMI
                     PVWIAVVFISILVHELGHAFAFRRFGQPSRILLHFSGGLTIPESMPWGGGYATVSLTP
                     NQHIFVSLAGPFAGFLLAGLILAVGVAMGGTLIFSTILKIIPFPIVMMPYGSGILTDI
                     FVTFLWVNIFWGIINLLPVYPLDGGNVARYLLIQRDPWNGLSTSLWVSVITGGLLA"
BASE COUNT          493 a          479 c          509 g          570 t
ORIGIN      
        1 caataaataa caattttcct ccaacacaaa ttttaccatt cgtataaaac ctgcatgtta
       61 tactctcgat atgccaaagg aacaagaaat aaattctatt gaaagaacag cagaagaatg
      121 ccaaggaaag ttacatccga aagccattgt aggtcttcag ttattcaatc aaggagaatt
      181 ttttgaagcc catgaagagt tggaaaccgc ttggcgagat gaaaccagct caattcgaga
      241 cttatatcgc ggcattcttc aaatcgcggt aacctatctg catattacac gcggaaacta
      301 tgacggtgca gttaaagtct atgggcggag cttgaagtgg ttgcaaggct ggggcgacat
      361 ctgccgcggt gttaacatca agaaacttcg ccgtgatgca gagaccgtta tgcaagaagt
      421 gactcgtttg gggcgggaaa gaatacgtga gttcgatagg tctttattcc aacctattca
      481 atggaacgaa aagcgcctgt ggctctgcga tcgttgcggc agtgagatgt atgaaaagaa
      541 ttgcaaagtg agttgcccaa cctgtggcaa ccgcttcgac tgttcggatt taaatcttta
      601 ctttgattaa tgctctggag acccaatgaa ttttcaaaac aaaatcgccc tcgtcaccgg
      661 ctcagggcgc ggaattggaa aagccatcgc cctgaatttt gccagtcagg gggcagatat
      721 tgtcgttaat ttctttcgca accgtgctcc tgccgaagag acagttcgcg agattgaaaa
      781 acttggcagg cgtgctttgc tggttaaagc cgatgtaggc gatttagatg acttaaatcg
      841 cctcttcgat gaagtagaaa aagaatttgg cggcttggat atcttcattc acaatgctgc
      901 atcaggctac aaccgccctg caatggaaca aaagcccaaa ggctgggatt ggacaatgaa
      961 catcaatgcg cgtgcattgt tattcgctgc acagcgtgcc acgcctctta tggaaaaacg
     1021 cggcggtggg agcatcgtca gcatatcgag tgcagggtcg gtgcgagtgt tacccgatta
     1081 tgttgtggtg ggcgcgagta aggcagccct ggaagccctc acgcgctatt taggtgtgga
     1141 gttgattcag cgtggcatca aggtcaacgc ggtgtcaccc ggcgtggtgg agacagaggc
     1201 gttgcaccat ttttcatcca tgggtgggca ggaaaacatc ctccagaaat tcatcgagcg
     1261 cgtaccggct ggtcgtctca tcactcctga agacgttgca gaagtggtag cgtttttgtg
     1321 ttcgcccgct tcttccatga ttgttggtca aaccatcgtc gtggatggcg gctatacttt
     1381 acccattcaa taacaggaca tattatgttt caagctgctc ctcccactcg ttttgattta
     1441 cagttttcaa tcgccggcat tcccgtgcgc gtgcatccgc tcttttgggt gattgctatt
     1501 ttgcttggct ctggctcaag caatttgttg atgatcccag tttggattgc agtagttttt
     1561 atttctattc ttgttcatga acttggtcat gcatttgcgt tcaggcgctt tggtcaacca
     1621 tcgcgcattc tcctgcattt ttctggtgga ttaaccatcc ccgaatcgat gccatggggc
     1681 ggcggatatg ccaccgtgag cctgacaccc aatcaacaca tcttcgtctc acttgccgga
     1741 ccgtttgccg gattcttgct cgcaggcttg atcctcgctg tgggtgttgc aatgggcggc
     1801 acattgattt tctctaccat actaaaaatc atccccttcc ccatcgtcat gatgccatac
     1861 ggcagcggta tcttgacaga tatctttgtg acctttttat gggtcaacat tttctggggc
     1921 atcatcaatc tcttgccggt ttatccactg gatggtggga atgttgctcg ttatctgctc
     1981 atccaacgcg acccgtggaa tggcttaagc acttcactat gggtttcggt catcaccggc
     2041 ggtttgcttg c
//
LOCUS       DAOQJE010000718         2049 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_43938_length_2049_cov_7.427783, whole genome shotgun sequence.
ACCESSION   DAOQJE010000718 DAOQJE010000000
VERSION     DAOQJE010000718.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2049)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2049)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2049
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43938_length_2049_cov_7.427783"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            173..715
                     /locus_tag="PLT08_15705"
     CDS             173..715
                     /locus_tag="PLT08_15705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE05972.1"
                     /translation="MKTPMFIAGAIIFSLALIDNPIYFQYLSLRFAYRFGLPLYFRVF
                     KVRPQANKFRIPRWQIEHWMVASGFSSPMAEKDEEGRYLFLETVSPLFRPFSLLMRGK
                     ISWDNPQKNVVVRGYATWSYFSILVLLVIGLLKAEGNICVGVVLFVYLLFCFMIYLYQ
                     MPHFYSIGERVVDYLSSHEE"
     gene            932..>2049
                     /locus_tag="PLT08_15710"
     CDS             932..>2049
                     /locus_tag="PLT08_15710"
                     /inference="COORDINATES: protein motif:HMM:NF024976.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction enzyme HsdR N-terminal
                     domain-containing protein"
                     /protein_id="HNE05973.1"
                     /translation="MTSQNAPAIIHKLVETFEQNLDSYRSSKNETELRREFLDKFFTA
                     LGWDVANEKGYDELGKEVIHEFSVDVSGQQKKADYAFRVGRGKNFDFLVEAKKPSLKI
                     ESNQDAAFQIRRYGWSAKLPINILTDFEHFAVYDCRTKPSYNDKASMGRIMLIHYKDY
                     VSRWNEIVEIFSPEAVRKGSFAKYAESMKGKKGTADVDDAFLQEIERWRELLAKNIAL
                     RNETIDIAGLNYSVQMTIDRIVFLRICEERGIEPENQLLEIVNRDGVYTELCELFKRA
                     DTKYNSGLFHFKQEKELVSRPDDLTLSLEIDDKVLKDIISNLYYPKSPYAFLYIPSDI
                     LGQVYERFLGKVIRLTTGHSAKVEEKPEVRKAGGVFYTP"
BASE COUNT          588 a          416 c          441 g          604 t
ORIGIN      
        1 caaaaacggg ctaacaaagc gtgcacctga cgctggggat tctgcgcaaa tctcaggcag
       61 tttcctacgc cttatcattt ttctggctgg acggcttcgc cgtccctgcc ccagcgcagg
      121 taacgcaaac cgttagccag cctgaataca ccgtaggcaa aatgagcatc ttatgaaaac
      181 accaatgttt atagcaggag ctatcatatt ctctctagct ttaatagaca atccaattta
      241 cttccaatat ctttcgttgc gttttgctta tagatttggt ctgcctctgt attttcgtgt
      301 tttcaaggtg cgtccgcaag ctaataaatt taggattcct cggtggcaaa tagagcattg
      361 gatggtagca tctggttttt catccccgat ggcagaaaaa gatgaagaag gacgatattt
      421 atttttggag accgtttccc ctttatttcg tcccttttcg cttctaatgc gcggaaaaat
      481 ctcatgggat aatccacaaa aaaatgtcgt tgttcgtggt tatgctacat ggtcatattt
      541 ttcaatattg gtattattag ttattggtct attaaaagcc gaagggaata tatgtgttgg
      601 tgtggtgctt ttcgtttatc tcttgttctg ctttatgatt tacttgtatc aaatgcctca
      661 tttttactct attggcgaaa gagttgtcga ttatttatct tcacatgaag aataacaaat
      721 caataaagaa gcgccttgca aaaatcggca ggctaacaca gcgtgcaccc gacgtgtggg
      781 agtctgcggc attttcaggc ttttttctgg cttcgggctt ttcctgttcc caagcattgt
      841 ccacgcccgc ccacacgcgg gtaacgcaaa ccgttggcaa gccccttgca caatagtctc
      901 atttttttat atccaaaggt aaaatcaggg aatgacatca caaaacgccc ccgcaattat
      961 tcataaattg gttgagactt tcgagcagaa tttagattct tatcgttcca gcaagaacga
     1021 aaccgaattg cgccgtgaat ttttggataa gttttttact gcattgggtt gggatgttgc
     1081 caatgaaaaa ggctacgatg aattagggaa agaagtcata cacgaatttt cggtcgatgt
     1141 ctcaggtcag cagaaaaaag ccgactatgc ttttcgtgtt ggtcgcggaa aaaattttga
     1201 ttttctagtt gaagcaaaaa agccttcgtt aaaaatcgaa tccaatcaag acgcagcttt
     1261 tcaaattcgc cgttatgggt ggagcgcaaa acttcccatt aatatcttga ccgattttga
     1321 gcatttcgcc gtttatgatt gccgaacaaa gccaagttat aacgacaagg cttcaatggg
     1381 gcgaatcatg ctcattcatt acaaagatta tgtttcaaga tggaatgaga ttgtagaaat
     1441 cttttcaccc gaagcagtac gcaaaggttc atttgccaaa tatgccgaaa gcatgaaagg
     1501 caaaaaaggt acagcggacg ttgacgacgc atttttacag gaaattgaac gctggcggga
     1561 attgcttgca aaaaatattg ccttgcgaaa tgaaacgatt gatattgcgg gcttgaatta
     1621 ttccgttcaa atgacgattg accgcattgt atttttgcga atttgtgaag aacggggaat
     1681 cgagccagaa aatcaattgc tggaaatcgt gaatcgtgac ggcgtttata ccgagttatg
     1741 cgagttattc aaaagagcag acacaaaata caactctggc ttattccatt tcaagcagga
     1801 aaaagaactc gtcagccgcc cagatgattt gacactttcg cttgaaattg acgacaaggt
     1861 tttgaaagac attatttcaa acttgtatta tccaaaaagc ccgtatgctt tcctttatat
     1921 tccttctgat attttagggc aggtttacga gcgttttctt ggcaaagtga ttcgcttaac
     1981 aacaggacat agcgcaaagg tagaagaaaa gcccgaagtc cgcaaggcag gcggtgtatt
     2041 ctacacccc
//
LOCUS       DAOQJE010000719         2049 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_43943_length_2049_cov_4.210632, whole genome shotgun sequence.
ACCESSION   DAOQJE010000719 DAOQJE010000000
VERSION     DAOQJE010000719.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2049)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2049)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2049
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43943_length_2049_cov_4.210632"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            332..1813
                     /locus_tag="PLT08_15715"
     CDS             332..1813
                     /locus_tag="PLT08_15715"
                     /inference="COORDINATES: protein motif:HMM:NF012422.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HNE05974.1"
                     /translation="MYIDDGQFSEREKEVIGLLLQGKSNKQIALSLGISASTVEYHLK
                     NVYKKLQVNSRTEAVLRLGKSIGNDTSGELRESTVEINGKPADNSDKFISTRRIPMNK
                     MFYIIEGVLLTTIVLVAIIGFANANIPVQNADVVPMTQASVIPTIATPTITSSSILLP
                     TEQILTNWQEATYTNTLNSSDITITLKWFYIDSTRVSMDFSVSGFVIPDGYVPLYQIV
                     NNVSLHTSNGDLITPDYDSIEWGAVRSNSESNTTGAKNIFDQTVFFPIKNRELAISQE
                     DSYIFDITVGGVPIYDNEGNATNEMLPSTTFHFEAKPSFVGSLTFVTEKAANIGDKVV
                     TLKGVEINPVFSIIMLCVFSPDNQQWIPNASLLYKGNVFNLSGARVTNEKNALSKELC
                     YRLEYDFQFDIMDNPKQSIAIWVNKLTKDQPERLPNELISSTFQKLSAKGIEFNYVIG
                     EHGSSIEITKKPIELTENEVSTMIQNALTENALTSGILIFDLK"
BASE COUNT          675 a          416 c          394 g          564 t
ORIGIN      
        1 ccaacaggct aaagttcaat taacgttaga agtattcgca gcaatatcag caactttgag
       61 ccaagcagaa aaagattctt acgttcaaaa attattacct attgtcgata cattagcact
      121 cagtaggctt gatttacttg ttgataagcc aaaaggcggc taacaaagcg tgcagcggac
      181 ggcggggagt ctgcgcgatt tataagcagt tttctggctt tgggtttttt ctacatctca
      241 aacagaatcc aaccccgccc gcgtccgccg ctaacgcaaa ccgttgggtg ctgggtcagt
      301 aagtcataat aggttcaagt gaaaacaaaa aatgtacatt gatgacggtc agttcagtga
      361 gcgtgaaaag gaagtaatag ggctactctt acagggaaaa agcaataaac aaatcgccct
      421 gtcgctcggt atttctgcaa gtactgttga atatcatttg aaaaatgttt acaaaaaact
      481 tcaagtaaat tctagaacag aagcagtttt gcgattgggg aaatccatag gtaacgatac
      541 gtcaggtgaa ttaagggaat ccacagttga aataaacggc aaacctgctg acaatagcga
      601 caaatttatt tcaacacgga gaattcccat gaataaaatg ttttacatta tcgagggcgt
      661 tttattaaca acaattgtat tggtagccat aattggattt gcgaatgcca atatacctgt
      721 tcaaaatgca gatgttgtac cgatgacaca agccagtgta atccccacaa tcgccacgcc
      781 aacaataact tcatcttcaa tacttttacc cacagaacag attttaacaa attggcagga
      841 agcaacttat accaatactc ttaattcatc tgacataact atcaccctaa agtggtttta
      901 tattgactca accagagtga gtatggattt ttcagtttcg ggatttgtta ttcctgatgg
      961 atatgtcccc ctataccaaa ttgttaataa tgtttcctta cacacttcaa acggcgactt
     1021 aattacccca gattacgaca gtattgagtg gggcgcggtc agaagtaata gtgaaagcaa
     1081 cacaactggg gcaaaaaata tttttgacca gactgtattt ttcccaataa aaaacaggga
     1141 attggctatt tctcaagaag attcgtatat ctttgatata acagtgggtg gcgtgccaat
     1201 ttatgacaat gagggcaatg ctacaaacga gatgttacct tcgacaactt ttcactttga
     1261 agcaaagcca tcttttgttg gttcattaac atttgtcact gaaaaagccg ccaatattgg
     1321 cgacaaagtt gtgacactta agggtgtaga aataaatccc gttttctcaa taattatgct
     1381 atgcgttttt tccccagata atcaacaatg gatacccaat gcttccttac tttacaaggg
     1441 aaatgttttt aacctttctg gtgctagggt aacaaatgaa aaaaacgctc ttagtaaaga
     1501 attatgctat cgcctggaat acgattttca atttgacatt atggacaacc caaaacaatc
     1561 aattgctatt tgggttaata aactgacaaa agaccagcct gaacgactgc caaatgaatt
     1621 aatatcttcc actttccaaa aactctctgc caagggaatt gaatttaatt atgtaattgg
     1681 agagcatggt tcaagtatag aaatcacgaa gaaaccaata gaacttacag agaatgaagt
     1741 atcaaccatg atacagaatg ctttaactga aaacgcatta acatctggaa tactgatatt
     1801 tgatttaaag taatacaacc tttacaattg caaaagtagg taattgttaa aagcgcccaa
     1861 caaagcgtgc acctgacgct ggggcgcttc gcgtgcgcac agtctcaagc agtttcctac
     1921 gccttatcat ttttctggct ggacggcttc gccggtccct gtcccagcgc aggtatcccc
     1981 tatcggggac gagcctggcg cacacacaac aaattcatga aaggcaatcc attacactcc
     2041 taaatgagc
//
LOCUS       DAOQJE010000720         2048 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_43977_length_2048_cov_12.506774, whole genome shotgun
            sequence.
ACCESSION   DAOQJE010000720 DAOQJE010000000
VERSION     DAOQJE010000720.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2048)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2048)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2048
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43977_length_2048_cov_12.506774"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..434)
                     /locus_tag="PLT08_15720"
     CDS             complement(<1..434)
                     /locus_tag="PLT08_15720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE05975.1"
                     /translation="MKYKNMISVLLISFILASCAPATIIVPSETAVPTSTFIIIPPTP
                     TITVTLDPMANVPEGTTGFDKTTGEPIREVTHEDGQTFTFHLNEQEEWVRLAGEFPLM
                     DIGQWNYIPFRITISEDTPGGSNILQIVHADSTDDNNRPITK"
     gene            complement(769..1971)
                     /locus_tag="PLT08_15725"
     CDS             complement(769..1971)
                     /locus_tag="PLT08_15725"
                     /inference="COORDINATES: protein motif:HMM:NF016847.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - transposition, DNA-mediated
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HNE05976.1"
                     /translation="MADIFRKYIGEYKKTHRLSYEQSKAVSAIINCRTPAMGGVMKTC
                     SVCKALEFHYKACKNRHCPLCGAFEKAQWLEAQKKWVLPIHYFQVVFTIDHVFNTLAA
                     FNQKLIFDLFIKVAAQLLKEFGQKYLGGEIGFSMVMHTWGQTMQEHLHLHFIVTGGAL
                     ISAPTGYSWNAASHKFLFPVKLLSNQFRNRFCFEISRLWVDGKLNTNDGALDVQAMLT
                     EGMSKNWEVYIQPPLYDKEKLIEYLGRYIFRIAISNHRIVAVGKDTVTFKYFDNRDDG
                     KEKLMTLPVMEFIRRFLSHVLPDGFVRVRHFGLHHASCRAKLQHARRVLGLPFQLPAT
                     LKLKFLDWFKQITKSDEDPRICKFCGQGLMLPIREFGPVFGWRLKAHALLGLLTSWKN
                     VLPNFVLG"
BASE COUNT          594 a          443 c          484 g          527 t
ORIGIN      
        1 cttttcgtta tcggacgatt attgtcgtct gttgaatctg cgtggacaat ttgtaaaata
       61 ttacttccac ctggagtatc ttcagaaatg gtaattctga atggaatgta attccactga
      121 ccaatatcca tgagcggaaa ttcacctgct agcctcaccc attcttcttg ttcattcaaa
      181 tgaaatgtga atgtttgccc gtcttcatgc gttacctctc gaatcggttc gcctgttgtc
      241 ttatcaaacc ccgttgttcc ctcaggaaca tttgccatcg ggtcaagcgt tactgtaatt
      301 gttggtgttg gggggataat tataaatgta gaagttggca cagccgtttc tgatggaaca
      361 ataatagtcg ctggcgcgca cgaagccaag ataaatgaaa ttagcaaaac tgaaatcata
      421 tttttatatt tcattcacta ctctgtttca taaggaactg cccaacggtt tgcgttactg
      481 gcgggcgggt gggcgtggaa actgtttggg aggaggacaa acttgaagcc agaaaaatgc
      541 ttgaaaattc gtactctccc cacccgtcca gtgcacgctt tgttggacga actgcttatt
      601 atagggattg cgtttttatt atagtcttaa aatgtagagt ggtaatcacc tgtttcgaga
      661 aagttgaaag aaaataaaag aggtgcgcga tcatgttagg gcaaaaggag ataaagcgag
      721 acataaaaac atcaagcagg cagagtccct cgcccgcaga cgcgcaaatc aacccagaac
      781 aaagttgggc aaaacatttt tccatgaagt gagtaaacca agaagggcat gagctttgag
      841 acgccagcca aagacgggac caaactcgcg gatgggcaac atcaagcctt gaccgcagaa
      901 cttgcaaatg cgtgggtctt catcagactt ggtaatctgt ttgaaccaat cgagaaactt
      961 gagtttaagg gtggcgggca attgaaaagg cagacccaaa acgcggcgag cgtgttgtag
     1021 tttggcgcgg caggaagcat ggtgtaaacc aaaatgacgg acacgcacaa agccatcagg
     1081 cagaacatga gagagaaaac ggcgaataaa ctccatcacg ggcagcgtca tcaacttctc
     1141 tttgccatca tcgcgattat cgaaatactt gaaagtgacc gtatctttgc caacagccac
     1201 aatgcgatga ttggaaatgg caatgcggaa gatatagcga ccaagatact caatcaactt
     1261 ctctttgtca tagagcggag gctgaatata gacttcccaa tttttggaca taccttcggt
     1321 cagcatagcc tgaacatcca acgcgccgtc atttgtattg agtttgccat ccacccagag
     1381 tcgggagatt tcaaaacaaa agcgattgcg gaattgattg gaaagtagtt tgacgggaaa
     1441 gaggaacttg tgcgaagccg cattccaaga gtagccagtg ggagcggaaa ttaacgcgcc
     1501 gcccgtaaca atgaaatgaa gatggagatg ctcttgcata gtttgtcccc aagtgtgcat
     1561 gaccatcgaa aagccgattt caccgccgag atatttctgt ccaaactctt tgagcagttg
     1621 agccgcaact ttgataaata aatcgaaaat gagtttctga ttgaacgctg ccagcgtatt
     1681 gaaaacatgg tcaatggtga agacgacttg gaaataatga attggcaaaa cccatttctt
     1741 ctgcgcttcc aaccattgcg ccttctcaaa tgcgccgcat aaagggcagt ggcgattctt
     1801 gcaggctttg tagtgaaatt ccaaagcctt gcacacggaa caggttttca tcacaccacc
     1861 catcgcgggc gtgcggcaat tgataatagc gctcacggct ttcgattgct catacgaaag
     1921 ccgatgagtt tttttatatt caccaatata tttgcggaag atgtcagcca catcgtattt
     1981 cacccctggc tgttgcgtcg atgtattttc tatatatcac ctatagttgt gcgaggttcg
     2041 tccaacgg
//
LOCUS       DAOQJE010000721         2047 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_44028_length_2047_cov_4.776104, whole genome shotgun sequence.
ACCESSION   DAOQJE010000721 DAOQJE010000000
VERSION     DAOQJE010000721.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2047)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2047)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2047
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44028_length_2047_cov_4.776104"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..459)
                     /locus_tag="PLT08_15730"
     CDS             complement(<1..459)
                     /locus_tag="PLT08_15730"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HNE05977.1"
                     /translation="MNLISPIVNRWIQDGKEDPDGFWEKAAKELYWFRVWDRVFEWNY
                     PNFRWFVGAQTNLSYNALDVHVKQGWGGHTALIYMNERDERRLFTYAKLLHDVEKIAA
                     ALRGMGISKGDRITIYMPTCPEAIMLMLATVRIGAIHSVVFAGFGEKALGD"
     gene            complement(525..1946)
                     /locus_tag="PLT08_15735"
     CDS             complement(525..1946)
                     /locus_tag="PLT08_15735"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNE05978.1"
                     /translation="MSQQSVTASADHEEAQSLPRIIFASAAGTMIEWYDFYIFGSLAT
                     VIASKFYQTGTDIGDIIAWLATFAIGFIVRPFGAIVFGRVGDLIGRKYTFLVTMTLMG
                     LCTFAVGLLPTIEVLGSLAGVLLITLRILQGLALGGEYGGAATYVAEHSPHGRRGFYT
                     SWIQITATLGLFMSLGVILATRKIVGEDAFKEFGWRIPFLISILLVAVSLWIRYSLRE
                     SPMYTKLKKSGGISKNPLKESFGNPYNLGFVGLALFGATMGQGVVWYTGQFYALFYLQ
                     KIFGVDLVDSNMIIGGALLFATPFFVFFGNLSDKIGRKNIMLTGMVLAVVTWLPIYQT
                     MAKYSPFIDFSSAADAVNTPNPAYSPFMLGVLIFIQVLYVTMVYGPIAAFLVELFPTK
                     IRYTSMSLPYHIGNGVFGGLVPIIGLELITKTGNNFAGLWYPTAIAAICAVICYFKIP
                     ETYKVDIESDASVAAIGAKVSGD"
BASE COUNT          599 a          504 c          472 g          472 t
ORIGIN      
        1 gtcacctaat gctttttcgc cgaaacccgc aaacactacc gagtgaattg caccaatgcg
       61 gacggttgct agcatcagca taatggcttc agggcaggtt ggcatataaa tagtaatgcg
      121 gtcgcccttg gaaatgccca ttccgcgtaa ggcggcagcg attttttcaa cgtcgtgaag
      181 cagctttgca tacgtaaaga ggcgacgttc atcacgctca ttcatgtaaa taagcgcggt
      241 atgacctccc cagccttgtt tgacatgcac atccaacgcg ttataagaaa gatttgtttg
      301 cgcccctacg aaccaacgaa aatttggata attccattca aaaacacgat cccacactcg
      361 gaaccaatat aactcttttg ctgccttttc ccaaaatcca tctgggtctt ctttaccatc
      421 ttgtatccaa cgattaacga ttgggctgat cagattcatg tctgtatctc cattgaggcg
      481 gagcgacaac atcgctccgc ctcaagttgt ccgtttgtat ttgtttaatc cccagagact
      541 tttgcgccaa tggctgctac agaagcatct gattcaatgt caactttgta ggtttcagga
      601 attttgaagt aacagatcac agcacagatc gctgcaatcg ctgttggata ccaaagccca
      661 gcgaagttat tgccggtctt tgtgatcaat tccaagccga tgatcggaac caagccgcca
      721 aagacgccat tgccgatgtg atacggaagc gacatggagg tgtagcgaat tttggtaggg
      781 aagagttcca ctaagaaagc cgcaatcggt ccatatacca tggtcacata gagcacttgg
      841 atgaagatca acacaccaag catgaaggga ctatatgccg ggttgggcgt attgacagca
      901 tctgccgccg aactgaaatc aatgaacggg gagtatttcg ccatggtctg gtaaatcggc
      961 agccaagtga caacagccaa gaccatgccc gtgagcatga tattctttcg accgatcttg
     1021 tcggataaat tcccaaagaa gacaaaaaat ggagtggcaa aaagcaacgc gccaccaatg
     1081 atcatattcg aatcgaccag gtccacgccg aatatttttt gcaggtagaa cagcgcatag
     1141 aattgcccgg tgtaccagac aacgccttgt cccattgtgg cgccaaagag ggctaagccc
     1201 acaaagccta aattgtaggg attaccaaag ctttccttga gcgggttctt ggaaatgccg
     1261 cccgatttct tgagcttggt gtacatcgga gattcgcgta gtgagtagcg gatccaaagt
     1321 gaaacagcca ccaaaaggat cgaaataagg aatggaatgc gccagccaaa ttctttgaag
     1381 gcatcttcgc ctacgatctt gcgcgtggcg aggatcacac caagagacat gaacaacccc
     1441 aatgtcgcag tgatttgaat ccagcttgtg tagaagccgc gcctgccatg cggtgaatgt
     1501 tcagctacat aagttgctgc gccgccatat tcgccgccca acgccagccc ttgcaaaatg
     1561 cgtaatgtga tcaaaagaac accagccaat gaaccaagaa cttcaatggt gggcaataaa
     1621 ccgaccgcaa atgtacacag acccatcaag gtcatggtaa caagaaaggt atatttccgc
     1681 ccaataagat caccaacacg tccaaataca atcgcgccaa acgggcgcac aataaaacca
     1741 atcgcaaaag ttgccagcca ggcaataata tcgccaatgt cagtaccggt ctggtagaac
     1801 ttactggcga tcacggttgc caaactgcca aaaatataaa aatcgtacca ttcgatcata
     1861 gtaccggcag ccgatgcaaa aataatacga ggtagacttt gtgcctcttc gtggtcagcg
     1921 gaggctgtta cagactgttg agacataatg caattctcct ttgggaagat caatggatat
     1981 gttccgcaca tggcggttgt aacaggctgg gtgatcacta ggcagaataa gtcatcagcg
     2041 ctccttt
//
LOCUS       DAOQJE010000722         2038 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_44410_length_2038_cov_7.189612, whole genome shotgun sequence.
ACCESSION   DAOQJE010000722 DAOQJE010000000
VERSION     DAOQJE010000722.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2038)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2038)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2038
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44410_length_2038_cov_7.189612"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1220
                     /locus_tag="PLT08_15740"
     CDS             <1..1220
                     /locus_tag="PLT08_15740"
                     /inference="COORDINATES: protein motif:HMM:NF024583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="HD domain-containing phosphohydrolase"
                     /protein_id="HNE05979.1"
                     /translation="DGRIRWVWDRAFPILNPNGTVRLVTGITTDITERKATDAKIQTQ
                     IKRLSALNSIDRAISSSMRMDVALDVLLIEVVAQLNVDAASILLLNETDQTLDFAAGK
                     GFNTSIEHASVPLGFELAGKVGTERKVIHVPNLSELKKGVKRKELIQEEHFLEYFGVP
                     LIVKGQLKGVLEIFNREALNPDPEWLTYLETLGGQAAIAIESAQLFEGLQRSNQELFM
                     AYDATIAGWSHAMDLRDKETEGHTQRVTELTVRLAESMGISQQQRIHIRRGSLLHDIG
                     KLGVPDHILLKPDKLTDEEWHIMRMHPVYARDMLTPINYLRPSLDIPYCHHEKWDGTG
                     YPRGLKGEDIPLAARLFAIIDVWDALRSDRPYRAGWSAEKTRDYIQSESGKHFDPKVV
                     EYFLALLEELSKS"
     gene            1420..>2038
                     /locus_tag="PLT08_15745"
     CDS             1420..>2038
                     /locus_tag="PLT08_15745"
                     /inference="COORDINATES: protein motif:HMM:NF024583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HNE05980.1"
                     /translation="MTAISKAMPKNKKGETGQKRQIKTSRANSPSAHHHIMNEVNLDI
                     NNKSKTAIQRLKAENARLQADLETLMRERRTMAETSLRQSQELDLLGRVRAAIATKLE
                     LPALLHSVVESIVEICGYAYVSIYLLEGETLILQHQAGYQKVIEKISIHEGISGRVAR
                     TGQPILIEDAKGEPEFLQASKKIRSEITVPLMDNGQVTGTLSIEST"
BASE COUNT          616 a          487 c          461 g          474 t
ORIGIN      
        1 cagatggtcg catccgctgg gtctgggatc gtgccttccc gatcctaaac ccgaatggaa
       61 cggtgcggct tgtgacgggt attaccaccg acatcacaga acgcaaggca accgacgcca
      121 aaattcaaac gcagatcaag cgtctcagcg ccctcaactc catagaccgg gcaattagtt
      181 ccagcatgcg catggatgta gctctggatg ttctccttat tgaagttgtc gcccagctta
      241 atgtggatgc cgcttccatc cttcttttga acgaaaccga tcaaacctta gacttcgcag
      301 caggcaaagg gtttaatact tccatcgaac atgcaagtgt gccgctcggg tttgaactgg
      361 ctggtaaagt tggcactgaa cgaaaagtaa ttcatgtacc caatctgtct gaattaaaaa
      421 aaggggtcaa aaggaaagag cttatccaag aagagcattt cctggaatac tttggtgtgc
      481 ccttgatcgt aaaaggacaa ctcaaaggcg tgcttgagat attcaaccgc gaagccttga
      541 acccagaccc tgaatggtta acctatcttg aaacactagg cgggcaagct gccattgcta
      601 ttgaaagcgc acaactattt gaagggttgc aacgctccaa ccaggaactt tttatggcat
      661 acgatgccac catcgcaggt tggtctcatg ccatggattt gcgtgacaaa gaaaccgaag
      721 gtcatacgca gcgcgtcaca gaactcaccg tccgcttagc agaaagcatg ggaatcagtc
      781 aacagcaacg gatccatata agacgcggat ccctcctcca tgacattggt aagctgggtg
      841 ttccagatca tattctgctc aagccagata aactcaccga tgaagaatgg cacattatgc
      901 gcatgcaccc cgtttacgcg cgcgatatgc tcacccctat caactatctg cgaccgtcgc
      961 ttgatatccc ttattgccat cacgaaaaat gggatggcac aggctatcca cgcggattaa
     1021 aaggtgagga tatcccgctg gcagccaggc tctttgcgat tatagatgtg tgggatgcgc
     1081 tccgctcgga tcgtccctac cgcgcgggct ggtctgccga aaagactcgc gactacatcc
     1141 agtctgaatc tggcaaacat tttgacccaa aagtcgttga atacttcctg gcacttctgg
     1201 aagaactttc aaaaagctga caattcaaca aaaaagacaa cccttgtggt atcacaaggg
     1261 ttgtcttttt ctattaggag tagatgttac ccatttttga gatttataac attgcagcat
     1321 cagcaaagag agcaatcata ttgtaatttc atctaacata gtgggtatgc tatactgatt
     1381 ttaagcccca gagacttgtg gagtagagga aataatggaa tgaccgccat ctctaaagca
     1441 atgcccaaga ataaaaaagg ggaaactggg cagaagagac aaataaaaac gagcagagca
     1501 aattcaccat ctgctcatca tcacatcatg aacgaagtaa acctcgatat taacaacaaa
     1561 tccaaaacag caattcagcg cctaaaagct gagaatgccc gtctgcaagc agaccttgaa
     1621 acgttaatgc gtgaacgccg cacgatggca gaaacgtctt tgagacaatc tcaagaatta
     1681 gatttattgg ggagagttcg tgccgccatt gctacaaaat tggaactgcc tgcactttta
     1741 cacagtgtcg tcgaatcgat tgtagaaata tgtggctacg cttatgtcag catttatcta
     1801 ttagaaggtg aaactttaat ccttcagcat caagcagggt atcaaaaggt catcgaaaag
     1861 atcagcatcc acgaaggcat tagcggacgt gtcgcccgaa ctggtcaacc cattcttata
     1921 gaagatgcaa aaggcgaacc ggaatttctt caagcctcaa aaaaaatacg ctccgaaatc
     1981 acggtgccat taatggacaa cggtcaagtg accggcacgt tgagcataga aagtacgt
//
LOCUS       DAOQJE010000723         2034 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_44572_length_2034_cov_4.246589, whole genome shotgun sequence.
ACCESSION   DAOQJE010000723 DAOQJE010000000
VERSION     DAOQJE010000723.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2034)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2034)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2034
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44572_length_2034_cov_4.246589"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>2034
                     /locus_tag="PLT08_15750"
     CDS             <1..>2034
                     /locus_tag="PLT08_15750"
                     /inference="COORDINATES: protein motif:HMM:NF024583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HNE05981.1"
                     /translation="DPNNGLLSPVTVSSTATAQLQANLNELKNDSAEESRTLDIELTE
                     GEKVLAQTKWVPSIQSGVALTVKSEVVFANINSLMPFTILLVLGSIFAMGVFLYYGTS
                     RAIKPLQSLSEMARKFAEGDRSLRADSINADNEIGQLSRSFNYLADQIEDHYTSLEKK
                     VEERTLQIRTAAEVAQSVTTIPNLDEMLRKTAELLVQQFNFYQTSVFLVDRSGKNIDF
                     KTGYGSGTKDLTERKYRVQVGSSSILGWVAANNKVRIASDTIEDELHLQNEFLPGTRT
                     EACVPISINDQVLGVLDVQSTQPSAFSADAILMLQTLASQLATAIQTAGLVDSSQVNF
                     QELDRLYRSSRLIEAAKDEAEILRVGGQILKPAPYPIVFLQLNSDSIRIINVSDMLRN
                     DLSAELTQNLQANIQELNSLLMRGSVIVSGTSVELSKPVKDILRAFGLLNAVAIPVKG
                     QEALKAIVLVGSQGEIPAGSSVQPYENLADLMSVALQRASAFVQTERHLQEVETLASM
                     NELIASTSDIQDFFHALHGKIQQIIGDYSLIVAIYDEPTNSISIPFSYEAGKIQAIPT
                     FPLGEGLTSILIRTRQPLLLVKDTERRAAELGAKIVGKPAKSWMGAPLIVQNTVTGAL
                     IIQDVEHEGSFNEDDLKFFTTIASQVAGVIKNVQLLDESKQKALQVETAAEIAR"
BASE COUNT          588 a          464 c          449 g          533 t
ORIGIN      
        1 agacccgaat aatggtttac tctcccctgt tacggtctct agtactgcaa cagcccaact
       61 tcaagccaac ttaaacgaat tgaaaaatga tagtgctgaa gaatctcgca ctttggacat
      121 tgaattaacc gaaggggaaa aagttcttgc acaaaccaag tgggttcctt ccattcaaag
      181 cggtgttgct ctgacagtta agtcggaagt tgtatttgca aacatcaaca gcttaatgcc
      241 tttcactatt ttattggttt taggttctat ttttgcgatg ggtgttttct tgtactatgg
      301 aacaagtcgc gcaattaagc ctttacagtc cctttctgaa atggctcgca aatttgcaga
      361 aggtgatcga agcctgcgtg ctgattctat aaatgcagat aatgagattg gtcaactttc
      421 gcggtcattt aattatcttg cagaccaaat cgaagaccat tacacctctc ttgaaaagaa
      481 agtggaagaa cgaacgcttc aaattcgaac tgccgccgaa gtggcgcaaa gtgttacaac
      541 cattcccaac cttgatgaaa tgctgcgcaa gaccgcggaa ctgcttgtac agcaattcaa
      601 tttttatcaa acaagtgtgt ttctggtcga tcgcagtgga aaaaatatcg atttcaaaac
      661 cggatacggt tctggtacaa aggatcttac agaaagaaaa tatcgagtac aagtcggctc
      721 ttcctcaatt ttgggctggg ttgctgctaa caacaaagtt cgcatcgcct cagacacaat
      781 tgaagacgaa ctacatcttc aaaacgagtt cctacccgga acacgtacag aagcctgtgt
      841 gcccatttcg atcaacgatc aggtattggg cgtattagat gtccaaagta cacaaccgtc
      901 ggctttcagt gccgatgcga ttctgatgct acagacattg gcaagtcagc tcgccactgc
      961 aattcaaacg gcagggttgg ttgactccag ccaagtcaac ttccaggaat tagatcgctt
     1021 gtatcgttcc agccgtttga tcgaagcggc aaaagatgag gcagagattt tacgtgtcgg
     1081 cgggcagatt ctaaaaccag ccccctaccc tatcgtcttc cttcaactaa atagtgacag
     1141 tatcaggatt atcaacgttt cagacatgct ccgaaacgac ctttctgctg agttgaccca
     1201 aaatttacaa gccaacattc aggaactaaa ttcattgttg atgcgtggtt ctgtcatcgt
     1261 atctggcaca agcgtggaat tgtccaagcc ggttaaagac attcttcgcg cctttgggct
     1321 tctcaatgcg gttgccatcc cagtaaaggg acaggaagcc ttaaaagcaa tcgtactggt
     1381 tggctcacag ggcgaaattc ccgccggttc ttcggtgcag ccatacgaaa acctcgcaga
     1441 cttaatgtct gttgcccttc aacgtgctag tgcctttgta caaacagaaa gacacttaca
     1501 agaggttgaa acactagcgt caatgaatga attgatcgcc tctacatctg atattcagga
     1561 tttcttccac gctttgcatg ggaaaatcca gcagattatt ggcgattaca gtttgatcgt
     1621 tgcgatctac gatgaaccga caaactctat cagcattcca tttagttatg aagctggcaa
     1681 aatccaggca attccaactt ttccacttgg tgaagggtta acatcaatat taattcgtac
     1741 gcgccaaccc ctattactgg ttaaggatac agaacgaaga gccgcagaac ttggagcaaa
     1801 gatcgttggg aagccagcca aatcttggat gggcgcgcca ttgatcgtgc agaacacggt
     1861 cacgggggca ttgatcatcc aagatgttga acatgaaggc tcgtttaatg aagatgacct
     1921 gaagttcttc actaccattg caagtcaggt agctggagtc atcaagaacg ttcagcttct
     1981 cgatgagagc aagcaaaaag cgttgcaagt agaaactgca gctgaaatcg ctcg
//
LOCUS       DAOQJE010000724         2030 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_44725_length_2030_cov_7.055696, whole genome shotgun sequence.
ACCESSION   DAOQJE010000724 DAOQJE010000000
VERSION     DAOQJE010000724.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2030)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2030)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2030
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44725_length_2030_cov_7.055696"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1314
                     /locus_tag="PLT08_15755"
     CDS             <1..1314
                     /locus_tag="PLT08_15755"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNE05982.1"
                     /translation="LITGKPFYESTDFFSPEWTEQNKDREGIKTLLDVPVFVDGEWWG
                     VIGFDDYQRVTPWSQAEVDALQVAASLLGSAIKRQRSTDELTASEDKFQKTFQQTMVP
                     MVIGRISDRVIVDANDAFAKLTGYSRAEVVNRWASELNLWQNNDEHALHHKLIRENGF
                     VHEFKTTLRKRSGDLGTVLLSVTMVRINNEDCLLYTISDISGLEKALNDLQNKNSELE
                     RFTYTVSHDLKAPLITIGGFMGLLEKDLASGNIDKINNSARRIKDAVTKMERLLNELL
                     ELSRIGRMINEPIKVSFEEILREAISLVQGRLSANKIEVQIEPELPFVKVDRARVVEV
                     IQNLLDNSAKFMGQQPNPKIAIGMRRTMDGERVFVVSDNGIGIEPVYHDRVFGLFNKL
                     DANTEGTGIGLALARRIIEYHGGRIWVESEGKGKGTSFCFTLPRP"
     gene            complement(1349..1430)
                     /locus_tag="PLT08_15760"
     tRNA            complement(1349..1430)
                     /locus_tag="PLT08_15760"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1394..1396),aa:Leu,seq:gag)
     gene            1496..>2030
                     /locus_tag="PLT08_15765"
     CDS             1496..>2030
                     /locus_tag="PLT08_15765"
                     /inference="COORDINATES: protein motif:HMM:NF014554.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE05983.1"
                     /translation="MARIVVDAMGSDEHPGPDVEGAIQAAREYGVEIIFVGDEAKIKP
                     VLEKQNTQGLSVRVVHAPEMLTMEDKGEALVLKARSKEPKNSMAIGMDLVKNGEADAF
                     VTAGNTGAGMVTALFRLGRIRGVDRPALAPIFPTATGTCVVLDIGANPDCKPENLIQF
                     GILGSIYAEKVRGVKNPK"
BASE COUNT          587 a          417 c          502 g          524 t
ORIGIN      
        1 cttattaccg gaaagccttt ttacgaatct actgactttt tctcgccaga atggactgag
       61 caaaataaag atagagaagg cataaaaacc ttactggatg ttccggtttt tgtagacggt
      121 gaatggtggg gggttattgg gtttgatgac tatcaacgtg tcacaccatg gtcgcaagcc
      181 gaagtggatg cattacaagt tgcagcaagc ttgttaggat cggcgattaa acgtcaacgc
      241 agtacagatg aattaaccgc ctctgaagat aaattccaaa agacctttca acagaccatg
      301 gttccaatgg tcattggcag gatctcagac cgggtcattg tagatgctaa tgatgctttt
      361 gcaaagctaa cagggtactc acgcgcagaa gttgttaatc gctgggcgtc tgagcttaat
      421 ctttggcaaa acaatgacga acatgcattg caccacaaac tcattcgaga aaatggtttt
      481 gtccacgaat tcaagactac tttgcgtaag agaagtggag acttgggcac tgtactcctt
      541 tctgtaacga tggttcgcat caataatgaa gattgtctgt tatatacaat ttctgatatt
      601 agtggtctag agaaggcgct taatgattta caaaataaaa actcggaatt agagagattc
      661 acatatacag tttctcatga cttaaaagcg ccactaatta caattggcgg atttatgggt
      721 ttgcttgaaa aggaccttgc ttctggaaat atagataaga tcaacaatag cgctcggcgt
      781 atcaaagatg ctgtcacaaa aatggagcgc ttattaaatg agcttctaga gctttcgcgc
      841 attggcagaa tgatcaacga accgatcaaa gtatctttcg aagagatttt gcgagaagct
      901 atcagtttag tgcaaggtcg attaagtgca aacaagatcg aagtccagat agagccggaa
      961 cttccttttg tcaaagtaga tcgcgcaaga gtcgttgaag tcatccaaaa tctactagat
     1021 aattctgcaa aattcatggg gcaacagcca aaccccaaaa tagcgattgg catgcgtagg
     1081 acaatggacg gtgagcgagt ttttgttgta tccgataatg gaatcgggat tgagcctgtt
     1141 tatcatgaca gggtgtttgg gttattcaac aaactggatg cgaatacaga aggaactgga
     1201 attgggttgg ctcttgcccg ccgaattatc gagtatcatg gtgggcgcat ttgggttgag
     1261 tctgaaggaa aaggtaaagg aacttcattt tgttttacgc tgccccgccc ttaaaacaaa
     1321 gaactcccat tttgggagtt cctgcttgtg cgctggagag gacttgaacc tccacggctt
     1381 gcgccacacg cccctcaaac gtgcctgtct gccagttcca gcaccagcgc gtaagcaggc
     1441 agtattataa tcgtgttact tttcttgtca agcaaatcat cataggagat ttttcatggc
     1501 tcgtatcgta gttgatgcaa tgggaagcga cgaacacccc ggacctgacg tagaaggtgc
     1561 cattcaggca gctcgggaat atggggtaga aattattttt gtaggcgatg aagccaagat
     1621 caagcctgtt cttgaaaaac aaaatacaca agggctaagt gtgcgagtag tacacgcgcc
     1681 tgaaatgttg acgatggaag ataaaggcga agcattggtt ttgaaggcgc gctccaaaga
     1741 gccaaagaac tccatggcaa tcggcatgga cctcgtcaaa aacggagaag cggatgcttt
     1801 tgtgacggct ggtaataccg gtgcaggcat ggtcaccgct ctcttccgcc tcggacgtat
     1861 ccgtggggtt gatcgcccag ccttagcccc catcttcccg acagcgactg gcacttgcgt
     1921 ggttttggat attggcgcca accctgattg caaacctgaa aaccttattc agtttggaat
     1981 tttaggaagc atctacgcag aaaaagttcg cggcgtgaag aaccccaaag
//
LOCUS       DAOQJE010000725         2026 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_44891_length_2026_cov_4.229325, whole genome shotgun sequence.
ACCESSION   DAOQJE010000725 DAOQJE010000000
VERSION     DAOQJE010000725.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2026)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2026)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2026
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44891_length_2026_cov_4.229325"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..817)
                     /locus_tag="PLT08_15770"
     CDS             complement(<1..817)
                     /locus_tag="PLT08_15770"
                     /inference="COORDINATES: protein motif:HMM:NF012693.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein phosphatase 2C domain-containing
                     protein"
                     /protein_id="HNE05984.1"
                     /translation="MRKFIPEIESSGLSIIGPVREDNQDTIHITDYPNSNGAGFLFAI
                     ADGMGGYAHGGMASALALESFLNVILTHHRQPVLRTLQRGVETANLHVYQKARQMDAG
                     RMGTTLTAAYILGDMLYLTHVGDSRAYLIRDGQATCLTSDHTTVGDMVRAKLIPAAKI
                     RTHAQRSILTKSIGTDLFVQPDILQQKLKPGDCLILCSDGLWSVVEDDDFARVASQSS
                     SVSEVSQNLVDLALAHHTDDNVSVVTFSVRSFMPVPAEQEPLPEESLGWFKKLR"
     gene            complement(951..>2026)
                     /locus_tag="PLT08_15775"
     CDS             complement(951..>2026)
                     /locus_tag="PLT08_15775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HNE05985.1"
                     /translation="GILADYLLRYFGYHPTVFGDALLAASFAAFAGFLALRGITGTTT
                     TSVILNTIQLLTLLTFGILAITFRLINPAAFAPTEWTYPSALAVLIPHSFDGLIFQMI
                     LAMILMVGFESSTALSAAATNAQRDIPRATILALIIQGLFSYLFGYFIAGFALNAHID
                     ATASQAPMGDIALQIGDLLLDGYGATLMVFFGFTVAIALLGGTLTAINNGVRISFAMA
                     LDEEMPDIFGMLHPRYTTPYNTVILLSIVCAIIGSAGIIGGMPVLMGIVLASNLGAFL
                     LYALLCLLTVVTFNGDPAFSFLRHLLVPVIGFLVNVGTVILAAKIGFSAGGLISQASW
                     VAWGLAGVWFIVSIIYYFLRRKA"
BASE COUNT          567 a          442 c          547 g          470 t
ORIGIN      
        1 tccttagctt cttaaaccac ccaagtgatt cttcaggtaa aggttcttgc tcggcaggaa
       61 caggcataaa gctgcgaact gaaaaggtaa ccacagatac attgtcatcg gtgtgatgcg
      121 ccagtgctag atcgactaga ttttggctca cttcagatac agatgaggat tgggatgcga
      181 ctcttgcgaa atcatcatct tccacaactg accataatcc atctgagcat aggatgaggc
      241 agtcacctgg tttgagcttt tgctggagga tatcaggctg tacgaataaa tcggttccta
      301 tggatttggt gaggatggag cgctgagcat gggttctgat cttggcggcg ggaatcaatt
      361 ttgcgcggac catgtctcct acggtggtgt ggtctgatgt aaggcaagtg gcttgaccgt
      421 cgcgaatgag ataggcacgg ctgtctccta catgtgtgag gtacagcata tctcccaata
      481 tgtaggcggc agtgagtgtg gtgcccatgc gccctgcatc catttgacgg gctttttgat
      541 agacatgcaa gttggcggtt tcaactccgc gctgtaaggt gcgtaaaaca ggctgtctat
      601 gatgtgtgag aattacgttt agaaaggact caagagctaa tgcgcttgcc atgcccccat
      661 gagcatagcc acccatacca tctgcgatgg caaagagaaa cccagcccca ttggagttcg
      721 gataatcagt gatatgaatc gtatcctgat tatcttctcg tacgggacct ataatgctca
      781 agccgctact ttcgatctcg gggatgaatt ttctcaaaac aagtcgcttt cacagtagtc
      841 cactcacagc aggcaaaatc aatccctgct gtgaggaaat aaagattaag caattctatg
      901 taagctggtt gtttgaaaat agtacccaaa tggggcattt cagttggcgt ttaggctttt
      961 cgtctcaaga agtaatagat gatactaact ataaaccata cgccagctag cccccaagca
     1021 acccaacttg cttgactaat gagtccaccg gcagagaaac caatcttggc tgccaagatc
     1081 acagtgccaa cattgactaa gaacccgatc accgggacga gaagatggcg cagaaaactg
     1141 aatgctgggt caccgttgaa tgtgacaacg gttaacagac agagcagagc ataaagtaga
     1201 aacgcgccga gattggatgc cagaacgatg cccattaata cgggcatgcc gccaatgatt
     1261 ccggcagagc cgattatggc gcagacaatg ctgagtaata taacggtgtt ataaggcgtg
     1321 gtgtatcgag gatgtaacat gccaaaaata tctggcatct cttcatccag cgccatggcg
     1381 aagctgattc gtacgccgtt gttgatggcg gttaatgtcc caccgagcag ggcgatggcg
     1441 acggtaaagc cgaaaaatac cattaaggtg gcaccataac cgtctaataa caagtcgccg
     1501 atttgtaggg caatatcgcc catgggtgct tgtgatgcag tggcatcaat atgggcgtta
     1561 agtgcgaatc ctgcaatgaa ataaccaaaa agatatgaga ataagccttg aatgattaac
     1621 gccagaattg tcgcgcgcgg aatgtcgcgt tgggcattgg tagccgcagc gctcaatgcg
     1681 gtagacgatt caaacccgac cattaggatc attgctagaa tcatttggaa aatcagcccg
     1741 tcaaagctgt ggggtatgag aacggctaag gcggagggat aggtccactc tgtgggggca
     1801 aatgcggcgg ggttgatgag gcggaatgta atcgcaagaa tgccgaatgt gagtagagtc
     1861 aataactgaa tggtgttcaa gatcacgctg gtggtggtgg tgcctgtaat tccgcgtaat
     1921 gcgaggaaac cagcaaaggc agcgaacgaa gcggctaaaa gggcatcgcc aaatacggtg
     1981 gggtggtacc cgaagtagcg cagcaaataa tcggcaagaa tgccca
//
LOCUS       DAOQJE010000726         2024 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_44983_length_2024_cov_4.120366, whole genome shotgun sequence.
ACCESSION   DAOQJE010000726 DAOQJE010000000
VERSION     DAOQJE010000726.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2024)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2024)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2024
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44983_length_2024_cov_4.120366"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1066)
                     /gene="vorB"
                     /locus_tag="PLT08_15780"
     CDS             complement(<1..1066)
                     /gene="vorB"
                     /locus_tag="PLT08_15780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate dehydrogenase subunit
                     VorB"
                     /protein_id="HNE05986.1"
                     /translation="MPKELWKGNEAIAESAVRAGLGAYFGYPITPQTEVLEYLSRRMP
                     ELGRAFVQAESELGAINMVYGAACTGVRVMSSSSSPGMSLMMEGLSYIAGTEIPAVLV
                     NVMRGGPGLGNIAPAQSDYNQAVRGGGHGDYPRIVLAPASVQEAIDLTALSFDLAEKY
                     RMITLIILDGTTGQMMEPAEMPAMQEMHRKNFEWATNGAMNRERRVLSSIYLNPLDEE
                     QANLRMLRNWKNVQANEVRYKEYFLDDAQYVVVGFGTAGRVALSAVREARAQGIKVGL
                     LRPITVSPFPFEALEQLIPQTEAFLVTEMNMGQMLDDVLMTVRGRVPVEFYGRPGGVV
                     PFHNEILDEIHRLASGPLAVT"
     gene            complement(1120..1362)
                     /locus_tag="PLT08_15785"
     CDS             complement(1120..1362)
                     /locus_tag="PLT08_15785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HNE05987.1"
                     /translation="MAAKGWIEVSDTYCKGCELCISACPQNVMELNMEHLTPKGYHPA
                     HTFKDGCTGCAICAVVCPDAAITVYREVTKAAPVAA"
     gene            1584..1832
                     /locus_tag="PLT08_15790"
     CDS             1584..1832
                     /locus_tag="PLT08_15790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE05988.1"
                     /translation="MENLKEWLLGLDTMILVILFLGGFIAWELKSGEVPLRWFGSIRR
                     DARPFFYWIAMLFHLAILGIVVYAYTDGVRMPLVEFFQ"
BASE COUNT          479 a          511 c          496 g          538 t
ORIGIN      
        1 tggtaacagc caaaggtcca gaagcgaggc gatgaatttc atccaaaatt tcattatgga
       61 acggaaccac gccgccaggt cgcccataaa actcaaccgg gactcgcccg cgcacggtca
      121 tcagcacatc gtcgagcatt tgccccatgt tcatctctgt gacgaggaac gcctcagtct
      181 gcgggatgag ttgttccagc gcttcaaacg gaaacgggct gacggtgatc ggtcttaaaa
      241 gcccgacctt gatgccttgc gcgcgcgcct cgcggaccgc cgaaagcgca acccgcccag
      301 ccgtaccaaa accaacgaca acatattggg catcgtctag aaaatattct ttgtaacgaa
      361 cttcattggc ttgcacgttc ttccagtttc tcaacatgcg caggttggct tgttcttcat
      421 ccaacgggtt gaggtagatc gaagaaagca cacggcgttc gcggttcatc gcgccgttgg
      481 ttgcccactc aaaattcttg cgatgcattt cttgcatggc tggcatttca gccggttcca
      541 tcatctgacc ggttgtgccg tcgagaatga tgagcgtgat catgcgatat ttttcagcga
      601 ggtcaaagga gagcgcggtc aggtcaatgg cttcttgcac ggatgcggga gccagcacaa
      661 tgcgtggata atctccgtgc ccgccgccac gcacagcttg gttgtaatca ctttgagccg
      721 gggcaatatt gcccaagccg ggtccgccgc gcatcacgtt gaccaacact gccgggattt
      781 ctgttcctgc gatataagac aacccttcca tcatcaggct catgcccggt gaagaggagg
      841 aagacatcac gcgcacgcct gtacatgccg cgccatagac catgttgatt gcgccaagtt
      901 cagattcggc ttgaacgaaa gcacgtccca gttcgggcat gcggcgcgag aggtattcga
      961 ggacttctgt ctgtggggtg atcgggtatc caaagtatgc ccccaaccca gcccgcacag
     1021 cagattctgc gatggcttca ttgcctttcc ataattcttt tggcattcgt ttctccatgt
     1081 aaccggtaag ggcgactcgc gagtcgccct tacgaataat tacgccgcaa ccggcgcagc
     1141 cttggtcact tcacgataga cggtaatcgc cgcatctggg cagacgaccg cacaaatagc
     1201 acaccccgtg caaccgtctt tgaacgtgtg cgcggggtgg tatcccttcg gagtgaggtg
     1261 ctccatattg agttccatca cattttgtgg acaggcactt atgcaaagtt cacagccttt
     1321 gcaataggta tcgctgactt cgatccaacc ttttgctgcc atcacagctc cttatattga
     1381 gaagttagac actattaggc gactttcatc gctaagtcta ttatgtcgtt ttcggaaatc
     1441 gaggagtagt gtcaggcgtc atgtacaagc gagtacaaag gtcattcgtt tttcggtcaa
     1501 ctacaaggac ttaagagcga cttgaacaga caatttcaga actctgagat gggtataatt
     1561 tcaaaaaatt taggagtgta tctatggaaa acttaaaaga atggttactc gggttagaca
     1621 cgatgatttt agttattctt ttcttgggtg gctttattgc gtgggaatta aaatctggtg
     1681 aagtcccttt gcgttggttt ggttcaattc ggcgtgatgc ccgtccattt ttttattgga
     1741 tcgccatgct gtttcattta gcaattctag gcattgtggt atatgcatat acagatggtg
     1801 ttcgaatgcc cttagtagaa ttttttcaat aaaaaagagc cgcaattgcg gctctttttt
     1861 atctcaatat tcttttgttg cgaatgtaag ttgttcaaca atcttccata gcgtttcgcg
     1921 attcgcttca tccatcactg caggtgacca cgccgcttct ttacaattcg tatcgaaata
     1981 tttccccgtc acaccttcga cctcaggcga agaggtgaga taga
//
LOCUS       DAOQJE010000727         2024 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_44987_length_2024_cov_3.773489, whole genome shotgun sequence.
ACCESSION   DAOQJE010000727 DAOQJE010000000
VERSION     DAOQJE010000727.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2024)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2024)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2024
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44987_length_2024_cov_3.773489"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            103..582
                     /locus_tag="PLT08_15795"
     CDS             103..582
                     /locus_tag="PLT08_15795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE05989.1"
                     /translation="MSFIFWIAVTIGIAIFLVPDFYTNRFQWAVESELVGLSPEYEGV
                     NKLHFRLAIFWFLFTLLLLPFLIVIVTNKTSQIETVMSSTMVLIFSGASLLRGIFAVV
                     KGVFPSIKYFGSRTLYAYSGNGEIKRFGRSVAIVSSVISALAIMSILINLLKQVMVF"
     gene            824..1978
                     /locus_tag="PLT08_15800"
     CDS             824..1978
                     /locus_tag="PLT08_15800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE05990.1"
                     /translation="MKNITYIFLSFAFLASCTNLSSLSATATPQATFTLTSTFTPTIT
                     PTITSTPTETPDPNKPIDATGKDPSTGEYTKIVEENSKTVVYVWKQFQFGDDSKNGVT
                     GHWFKSWMANEPINLTGYGENCLNTWGTSTALAINMNVYAIEGQTDLDKIGYIFHPDR
                     ELEWNKYMDAKGEGVSCSTISLPNVVMSDLFQRHINLPPGDPNDVSSHNFLDYYYPDG
                     KFTDDGAQRFINDLQTFAEAINNDNLTIKVGSDIWYPHKGYEVYWIDENMAVNDTTMH
                     LALWQYPSTKHFYWKIMIKDGKLLAFIAPADILKKQLAEPKAESRKKWFRTMILAPIK
                     VVNIGMNINEVDSLISSVDDYGGITGILSGTINGKTLYIDKPFIDFTSIK"
BASE COUNT          607 a          413 c          407 g          597 t
ORIGIN      
        1 gctggacggc ttcgccgtcc ccgccccagc gcaggtaacg caaaccgtta ggcgcacact
       61 ctggataact actatgaata acaaaatagg ttaggatatt ctatgagttt tattttttgg
      121 atcgcggtta ccatcggaat agccattttc ttagtcccag atttttatac aaacagattc
      181 caatgggctg tagaatcaga gcttgtggga ctttctccag aatacgaagg tgtaaacaag
      241 ttgcatttca ggctagcaat tttttggttc ttattcactt tgttattatt gcccttccta
      301 attgttattg tcactaacaa aactagccag attgaaacag ttatgtcctc cacaatggtt
      361 ttgatttttt caggtgcatc actccttcgg ggtatttttg cagtagtaaa aggtgttttt
      421 ccgtcaatca agtattttgg ctcaaggacg ttgtatgctt actctggcaa cggagaaata
      481 aagcgttttg gtcggtcagt cgcaatcgta tcaagtgtta taagtgcatt agcaatcatg
      541 tcgattttga taaatttgtt gaagcaagtt atggtttttt agttattgcc aaagttttta
      601 ttgtaggtaa aatattatca agatgggttt ggtgttcgtt atcaataaag aaagttggcg
      661 ttccgtgagt gcgcctaaca aagcgtgcac ctgacgctgg ggattctgcg gcaatctcag
      721 gcagttttct acgccttatc atttttctgg ctggacggct tcgccgtccc tgccccagcg
      781 caggtaacgc aaaccgttgg cttgcccctt tgaaaaggaa gttatgaaga atattaccta
      841 catctttctc tcgtttgctt ttctagcatc ttgtactaat ttatcttcat taagtgcaac
      901 tgccacccca caagcgactt ttacactaac atctactttc actccaacaa taacgcccac
      961 tatcacttct actcctaccg agacccctga cccaaataag cctattgacg cgacaggtaa
     1021 agacccatcg actggagaat acaccaaaat cgtcgaagaa aatagtaaaa ctgtagttta
     1081 tgtttggaag caatttcaat tcggtgacga ttcaaaaaat ggcgttactg gacattggtt
     1141 taaatcatgg atggcaaatg aaccaattaa cttaacagga tatggcgaaa attgcctaaa
     1201 tacttggggg acatctactg cactcgctat taatatgaat gtttatgcga ttgaaggtca
     1261 aacagacctc gacaagattg ggtatatttt ccatcctgac agagaactag agtggaataa
     1321 atacatggac gcaaaaggtg aaggcgtgtc gtgcagcacc atttctctgc caaatgttgt
     1381 tatgtccgat ttattccaaa gacatatcaa cctaccccct ggagacccaa atgacgtatc
     1441 ttctcataat ttcttggatt attactatcc cgatggaaaa tttactgatg atggggcgca
     1501 acgttttatt aatgacctgc aaacatttgc agaagcgatt aataatgaca acctaacaat
     1561 aaaagttgga agtgacatct ggtatcctca taaaggatat gaagtctact ggattgatga
     1621 aaatatggcg gtaaatgaca caaccatgca tttagcgtta tggcaatacc catcgacaaa
     1681 acacttttac tggaagataa tgattaagga tggaaaattg cttgccttca ttgctcctgc
     1741 tgacatcctg aaaaagcaac ttgccgaacc aaaggctgag agcagaaaaa aatggtttcg
     1801 cacaatgatt ctcgctccca taaaggtggt gaatatcggc atgaatataa atgaggtaga
     1861 tagtttaata tcttcggtag acgactatgg aggaatcaca ggcattcttt ctggcacgat
     1921 aaacggaaaa actctttata tcgataaacc attcattgac tttacctcta ttaaatgaga
     1981 gatcctcaca taaaagcaag ccaacaaagc gtgcacctga cgct
//
LOCUS       DAOQJE010000728         2022 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_45075_length_2022_cov_3.532283, whole genome shotgun sequence.
ACCESSION   DAOQJE010000728 DAOQJE010000000
VERSION     DAOQJE010000728.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2022)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2022)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2022
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45075_length_2022_cov_3.532283"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..534
                     /locus_tag="PLT08_15805"
     CDS             <1..534
                     /locus_tag="PLT08_15805"
                     /inference="COORDINATES: protein motif:HMM:TIGR01494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HNE05991.1"
                     /translation="RNEAAPTMKALKGIGVAHTIMLTGDNARSASAIAKETGLTEFRA
                     DLMPDDKLTIIRELVKEYGHVAMIGDGVNDAPALANATVGIAMGGAGTDVALETADVA
                     LMGDDLSKLPFAVGLGRATRAIIVQNLLISLGVIALLIITSLTGIVSIGIAIIFHEGS
                     TLVVVANALRLLGYKSA"
     gene            612..863
                     /locus_tag="PLT08_15810"
     CDS             612..863
                     /locus_tag="PLT08_15810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE05992.1"
                     /translation="MDVAVMILSGISLLSVAIIGFDTIHVEGSLAVLMVILSPLMLIT
                     GIILAWQGHGYILFLLAVPALGLGILGARKLMDKREKHD"
     gene            856..1464
                     /locus_tag="PLT08_15815"
     CDS             856..1464
                     /locus_tag="PLT08_15815"
                     /inference="COORDINATES: protein motif:HMM:NF014667.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCO family protein"
                     /protein_id="HNE05993.1"
                     /translation="MIKKNHYYLLFIMMAVIALGVLIEVVFRRPYSFHGTTINPPLPL
                     TDFALQTANEEVFRLSEQKGKIVLLFFGYTSCPDVCPTTLATFKQVYERLGDDAQKIK
                     FVMITADPERDTPDKVAEYAARFNPEFIGLSGDITKREAIWKELGVFVEKQDSGSAAG
                     YLVSHTSSVYVLDQSGDLIMTFPYGTSATDMVNDLTQLLKQE"
     gene            1482..1916
                     /locus_tag="PLT08_15820"
     CDS             1482..1916
                     /locus_tag="PLT08_15820"
                     /inference="COORDINATES: protein motif:HMM:NF016221.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016531 - copper chaperone activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper chaperone PCu(A)C"
                     /protein_id="HNE05994.1"
                     /translation="MKNIALISVFMLATLLVSACGSPVGELTIQDAWARPATAGENSA
                     AYFIIENKTASDDTLMSVSSDIATAAEVHMSMADANGVMSMQMQEAVTIPAGEQVEFK
                     PGGLHVMFVNLTRDLKPGEAITLTLNFKETGNVVVEVPVKAP"
BASE COUNT          494 a          469 c          530 g          529 t
ORIGIN      
        1 cggaatgaag ccgctccgac tatgaaggcg ctcaaaggaa taggcgtggc tcatacgatt
       61 atgctgacag gcgacaacgc gcggtccgca tccgccatcg ccaaagagac tggcttaacg
      121 gagttccgtg ccgacctaat gcctgatgat aaattgacta tcattcgtga gcttgtgaaa
      181 gaatatggtc atgtcgccat gatcggcgac ggcgtgaacg atgcgcccgc tctggcaaat
      241 gcaactgtgg gcatcgcaat gggtggcgcg ggtacagacg ttgcattgga aactgccgat
      301 gttgccctga tgggtgatga tctttccaaa ctaccgtttg ctgttggatt gggtcgcgct
      361 acgcgtgcga ttatcgttca gaacctcctc atctcgctgg gtgtgatcgc cctgctcatc
      421 atcacttccc taacgggtat tgtcagcatc ggtattgcca tcatcttcca tgaaggcagt
      481 acgttggtcg tcgtggcaaa tgccttgagg ttattgggtt ataaatctgc atgaaatggc
      541 actggagtct gctatttgcg ggttttgttt tgtacctcct tgttctgatt ccaggagtcc
      601 tgccgcactc cgtggatgtt gcggtaatga tcctatcagg tatcagtctg ctttccgttg
      661 ccatcattgg atttgatacg atccatgtgg aaggaagcct ggctgtcctg atggtcatcc
      721 ttagcccgct tatgctaatc acaggaatta tcctggcgtg gcaagggcat ggctatattc
      781 tgttcttact cgccgtacca gcgcttgggc ttggtatatt gggcgcacga aaattaatgg
      841 ataaaagaga gaaacatgat taagaagaat cactattacc tgctgtttat catgatggca
      901 gtcattgctt tgggggttct gattgaggtc gtgttcagaa gaccctattc ctttcacggc
      961 acgactatca accctccact gcccttaacc gattttgccc ttcaaaccgc caacgaagaa
     1021 gtatttcgac tgagcgaaca aaagggcaag atcgtattac tctttttcgg gtataccagt
     1081 tgccccgatg tttgtccaac tacgctggcg actttcaagc aggtgtatga aaggctgggc
     1141 gatgatgcgc aaaagatcaa gtttgtgatg attactgccg accctgagcg cgatactccc
     1201 gataaagttg cggaatatgc ggcgcgtttc aatcccgaat tcatcgggct gagcggagac
     1261 attactaaac gtgaagcgat ttggaaggag ctgggagtat ttgtcgagaa gcaggattca
     1321 ggaagcgcgg caggctacct cgtgagtcac acttccagcg tctacgttct agaccagagc
     1381 ggagatctga ttatgacctt tccatacggc acaagtgcaa ccgacatggt gaatgacttg
     1441 actcaactct tgaaacagga ataaatccaa aaggaacgtt catgaaaaac atagctttga
     1501 tttccgtatt tatgctggca actttgcttg taagtgcctg cggctccccg gtgggggaat
     1561 tgaccattca agatgcctgg gcgcgtcccg ccaccgcagg tgaaaacagc gcggcttatt
     1621 tcattatcga aaacaaaacg gcgtccgatg acactttgat gagcgtcagt tctgatattg
     1681 ccaccgccgc tgaggttcac atgagcatgg cggatgcaaa cggggtgatg tccatgcaga
     1741 tgcaggaggc agtaacaatc cctgctggtg aacaggtgga gttcaaaccg ggtggactgc
     1801 atgtgatgtt cgtcaatctg acgcgagatt tgaaaccggg tgaagcgatt acactcacat
     1861 tgaatttcaa agaaactggt aatgttgtag ttgaagttcc cgtgaaagcc ccctaatcca
     1921 actatcatgt atcgagttgc catcagcgga tataatttca gtctgatggc aactccggtg
     1981 atgacatgaa attacaccaa cgaatttttc tggcggtgac tt
//
LOCUS       DAOQJE010000729         2020 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_45196_length_2020_cov_2.349618, whole genome shotgun sequence.
ACCESSION   DAOQJE010000729 DAOQJE010000000
VERSION     DAOQJE010000729.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2020)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2020)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2020
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45196_length_2020_cov_2.349618"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2020)
                     /locus_tag="PLT08_15825"
     CDS             complement(<1..>2020)
                     /locus_tag="PLT08_15825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE05995.1"
                     /translation="FAYAVKNGKRIIPIVIRETDLSSDNKTSNRNCIFCRAEQDDFAQ
                     AIQEIVDTIYTDYEWVKYHTELQVKAVRWKQSHDENSFLMRGKELKVAEEKLGIPRRG
                     KDLKPTVLQQEFVSASRKHETKRQRLLFTGLFTGLTVALALFVIAVLAFIFGRNQQLI
                     AEKEAVAKATAQSAAQSQGEIASGRELASQAQLLPLYGNSMIVSGLLSIEAANLDSSI
                     QVQQSLSRVANSIGNPIERYSLENTILSGAISLDGKFIAAGDAKGNLNIWDAKTDQII
                     ANYQHKGSVLSIAFSPIENKVLSIGNDLVVWDLTTGKALLHLDEPTSIFQSALFSADG
                     NTIIALSELDNDQNTLIAIDLLSGNIISRFVFDGTAPKLSLSQQNSWAVIAQNYDNEK
                     LRLQVWDWRREQKVAEKDFDVTYNVYGYQIDNSKILSVDSDVTVVMWDVYTGNNILSG
                     EVCKSAIRTMILSTNAEKVAIGCDEGTIKVLDTQNLNELMSVSQDENTDDLTFNPVFA
                     MGFNADASRLITGGSDGTAIVWDIETQTELARMPHEGSIQQVAFNPVENWVISWGRET
                     MITAWIPANGLSTLPIVQEHAIQALSFDPEGNLLVASYGGNIQKWDISRQQMLLGNNV
                     SVLTLMPVVFSKDGTLMIADTDDNIVRVFNVISGEEISSVTHTDWVDSI"
BASE COUNT          568 a          469 c          354 g          629 t
ORIGIN      
        1 tattgaatcg acccaatctg tatgggtaac agacgatatc tcctctccgc tgatcacatt
       61 aaacactcta acgatattgt cgtcagtatc tgcaatcatc aaagtcccat ccttgctaaa
      121 caccactggc atgagagtta acacagaaac attatttcca agaagcattt gctgccttga
      181 aatatcccat ttctgaatat tcccaccata actagccaca agtaaattgc cttccggatc
      241 aaaagataaa gcttggatgg catgttcctg aacaattgga agagtgctta atccattagc
      301 aggtatccat gcagtaatca tcgtttctct tccccatgaa ataacccaat tttctactgg
      361 attaaaggca acttgttgaa ttgacccttc gtgtggcatt ctagccaact cagtttgggt
      421 ttcaatatcc catacaattg cagtcccatc tgatccgcca gtgattaagc gacttgcatc
      481 tgcattaaag cccattgcaa aaacagggtt aaaagtcaga tcatctgtgt tttcatcctg
      541 cgaaacgctc attaattcat ttaaattttg tgtgtcaagg acttttattg ttccttcatc
      601 acaaccaatt gcgacctttt ctgcattggt acttagtatc atagttctta ttgctgattt
      661 gcagacctca cccgaaagaa tattgttgcc agtataaaca tcccacatca ctacagtaac
      721 atcactgtcg accgataaaa tcttggaatt gtcaatttga tatccataca cattgtaggt
      781 aacatcaaag tctttttcgg cgaccttctg ttcccttctc caatcccaaa cctgtaaacg
      841 caacttttca ttatcataat tctgggcaat cacagcccat gagttctgct gactcaacga
      901 aagctttggc gctgtgccgt caaacacaaa ccgagaaatg atattcccag ataataaatc
      961 aatcgcaatc aatgtgtttt ggtcattatc cagttctgat aaggcaatga ttgtattgcc
     1021 atctgctgag aataatgctg attgaaagat tgaagtaggt tcatccagat gaagcagcgc
     1081 ctttcctgtt gttagatccc atacaacgag gtcgttcccg attgataata ctttgttttc
     1141 aataggacta aaggcaatag acagtactga gcctttatgt tggtaattcg caataatctg
     1201 atcagtttta gcatcccaga tatttagatt accttttgca tcccccgcag caataaattt
     1261 cccgtccaaa ctgatagctc cagataagat cgtattttct agtgaatatc tttcaatggg
     1321 gttcccaata gaatttgcaa ctctcgataa tgattgctga acttgtatgc ttgagtcgag
     1381 atttgctgcc tcaatggata gcaaaccgct cacaatcata ctgtttccat acaacggcaa
     1441 caattgagcc tgagatgcca gttctcttcc tgaggcgatt tccccttggc tttgcgcagc
     1501 cgattgagct gtcgccttgg caacagcctc tttttctgca attaattgct gattacgtcc
     1561 aaaaataaat gctagcacag caatcacgaa aagtgccaga gccactgtca aacccgtgaa
     1621 tagccctgtg aataacagtc tttgtctttt tgtttcatgt tttcggctag ctgatacaaa
     1681 ctcttgttgt agtacagttg gtttaagatc cttaccgcgc ctcggaattc ctaatttttc
     1741 ttccgcgact tttaattcct ttcctcgcat taagaagcta ttttcgtcat gagactgttt
     1801 ccaccgaact gcctttacct gcaattcggt atgatatttc acccactcat aatccgtata
     1861 aatagtatca acgatttcct gaattgcttg ggcgaaatca tcctgttcgg cacggcaaaa
     1921 aatacagtta cggtttgaag ttttattgtc agagctcaaa tcagtttcac ggataacaat
     1981 ggggatgatc cgcttgccgt ttttaaccgc gtaagcaaat
//
LOCUS       DAOQJE010000730         2017 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_45300_length_2017_cov_5.670234, whole genome shotgun sequence.
ACCESSION   DAOQJE010000730 DAOQJE010000000
VERSION     DAOQJE010000730.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2017)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2017)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2017
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45300_length_2017_cov_5.670234"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..405)
                     /locus_tag="PLT08_15830"
     CDS             complement(<1..405)
                     /locus_tag="PLT08_15830"
                     /inference="COORDINATES: protein motif:HMM:NF015013.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid II flippase MurJ"
                     /protein_id="HNE05996.1"
                     /translation="MTETKSNANRQIARAAGTVMFAFVLSNIMGLVRQILVSKTFGTN
                     QSLDAFYAAATFPDLLFSLLAGGALASAFVPTFTGFLANEKKEDAWRLASAVTNLVIL
                     LLSLVSGLAALFAPGIVRHVLFVLKPELDPSLQ"
     gene            430..1548
                     /locus_tag="PLT08_15835"
     CDS             430..1548
                     /locus_tag="PLT08_15835"
                     /inference="COORDINATES: protein motif:HMM:NF024491.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase D-like domain-containing protein"
                     /protein_id="HNE05997.1"
                     /translation="MKKTSTSLFLLVILSLACSSTTLTPDVAPQTPPTVDSTDPASAT
                     LTEIPLPVGYGAKNSWIEIYFTDPSNSLSSQKTGGIDLPLADAIDSAKLTVDVAIYSL
                     SLNSIRDALLRAQDRGVQVRMVMESENLDRSDPQRLLDAGIPILGDRREGLMHNKFTV
                     IDGSEVWVGGMNYTDSGTYEDNNVLMRIRSTKIAENYTKEFEEMFVDDKFGDNVLAET
                     PNPRVTMDGTPVDTYFSPDDGVQSILLDILNEAQSSIYFMAFSFTSDELGDAILQRAE
                     SGVTVAGVMDEEQVQSNQGTEYDAFRRAGLDVYIDGNPGQMHHKIMIVDESIVIVGSY
                     NFTNSAETRNDENLMVIYDKDIAAQFLNEFQRVYGQAK"
     gene            1672..>2017
                     /locus_tag="PLT08_15840"
     CDS             1672..>2017
                     /locus_tag="PLT08_15840"
                     /inference="COORDINATES: protein motif:HMM:NF039822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5666 domain-containing protein"
                     /protein_id="HNE05998.1"
                     /translation="MKKQSYIFLVLVALIAVFVSACGGAAAATDTSAGSDKPRASLVE
                     FTGVIEAIDGNQWTVNGQVITVDPTVLRDGPFVVGDTVKVEAEVQSDGSVVITRVEPP
                     VVAVGTDDISNDN"
BASE COUNT          519 a          465 c          529 g          504 t
ORIGIN      
        1 ttggagggaa gggtcaagct cgggcttgag aacgaataga acatggcgaa caattcccgg
       61 cgcgaagaga gccgcgagtc cgcttacgag gctgagtaac agaatcacca gattcgtcac
      121 ggcagaggcg agccgccagg catcctcttt tttttcattc gcaagaaacc cagtaaaagt
      181 tggcacgaag gctgaagcca atgccccgcc tgctagcaaa ctaaaaagca ggtcagggaa
      241 agtggctgcg gcataaaagg catcgaggct ttgattggta ccgaaagttt tcgaaacgag
      301 gatctgacgc accagcccca taatgttgct caacacaaaa gcgaacatca ccgtccctgc
      361 ggcacgtgcg atctggcgat tggcgtttga tttggtttct gtcatggcgg caagattata
      421 atgtttttca tgaagaaaac atcgacaagc ctctttctcc ttgtgatcct ttcactggcg
      481 tgcagttcta ctactctcac gccagacgtt gcgcctcaaa ccccgccgac tgttgactct
      541 acagacccag cctctgccac actgacggag attcccttac cagttgggta tggtgccaaa
      601 aattcgtgga tcgaaattta tttcaccgac ccatcgaatt cattatcgtc tcaaaagact
      661 ggcggcattg acctaccctt ggcggatgcc attgactctg ctaagttgac ggtagatgtt
      721 gcgatttata gtttgagtct gaatagcatt cgtgatgcgt tgttgcgtgc gcaagatcgt
      781 ggcgtgcaag tgcgcatggt gatggaaagc gagaacctcg accgttctga cccgcaacgt
      841 ttgcttgatg cgggcatacc tattttggga gatcgccgcg aaggcttgat gcacaataag
      901 tttacggtca ttgatggttc tgaggtatgg gtggggggca tgaactatac cgactcagga
      961 acatacgaag ataacaacgt gctgatgcgc atccgctcga caaagatcgc tgaaaattac
     1021 accaaagaat tcgaagaaat gttcgtggat gataaatttg gcgataatgt ccttgccgaa
     1081 acgcccaatc cgcgtgtgac gatggatggt acgccagtgg atacgtattt ctcgcccgat
     1141 gatggtgtgc aatctatttt gctcgatatt ctcaatgaag cgcaatccag catttacttt
     1201 atggcgtttt cttttacatc tgatgaatta ggcgatgcca tccttcagcg tgcagaatcc
     1261 ggcgtcactg tagcaggggt gatggacgag gaacaagtcc agtcgaatca aggaaccgag
     1321 tacgatgcct tccgtcgagc cgggttggat gtgtatatcg acggcaaccc cgggcagatg
     1381 catcacaaaa tcatgatcgt ggacgaaagc atcgtgattg tcggttcgta caattttacg
     1441 aatagcgccg aaactagaaa cgatgagaat ttgatggtga tctacgataa agacattgcg
     1501 gctcagttct taaatgaatt tcagcgagtg tatggtcagg caaaatagac tttaacctgt
     1561 attttattct ttacggctcg tccctaaaat tgcacagggg cgagccgtaa cttttatgta
     1621 ccctctacga ctatctgggt gtagccaaat aaaaaaagaa gaggtgtaaa catgaaaaag
     1681 caatcttata tcttcctggt tctggtagcg ttgatcgcag tttttgtcag tgcctgtggt
     1741 ggggcagccg ccgccaccga tacatcggcg ggttcagaca agccgcgtgc gtcactagtt
     1801 gaatttaccg gcgtgatcga agccatcgat ggtaaccagt ggaccgtcaa cggacaggtc
     1861 atcaccgtgg accccacagt cctgcgcgat ggtcctttcg tagtgggtga tacggtcaaa
     1921 gtagaagctg aagtacagtc tgatggttct gtggtgatca cccgtgtgga acccccggtt
     1981 gtggctgtag gtaccgatga tatttccaat gacaatt
//
LOCUS       DAOQJE010000731         2013 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_45459_length_2013_cov_5.279877, whole genome shotgun sequence.
ACCESSION   DAOQJE010000731 DAOQJE010000000
VERSION     DAOQJE010000731.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2013)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2013)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2013
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45459_length_2013_cov_5.279877"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(187..789)
                     /locus_tag="PLT08_15845"
     CDS             complement(187..789)
                     /locus_tag="PLT08_15845"
                     /inference="COORDINATES: protein motif:HMM:NF015423.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trimeric intracellular cation channel family
                     protein"
                     /protein_id="HNE05999.1"
                     /translation="MDQLLLILTYIAVIASSVSGALGARKYQMDIVGAVTIAYVNAFG
                     GGTVRDLLLGRTPIFWIADPALTVTTLIVAIISFYRLDRISKLLLTTADAIGLGIFSI
                     LGATYTLQLNLSPIVAIHMGVVTGIFGGVLRDVLSNRIPKVFGQSTELYATCSFVGTT
                     LFIILYVLHVNTLLASLAGALTIFTMRLLAVRFKMTLPSP"
     gene            complement(864..>2013)
                     /locus_tag="PLT08_15850"
     CDS             complement(864..>2013)
                     /locus_tag="PLT08_15850"
                     /inference="COORDINATES: protein motif:HMM:NF013630.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="LysM domain-containing protein"
                     /protein_id="HNE06000.1"
                     /translation="TESYLGISGILVDNTLDWSNIQYVADASSMGGGGGGGGFGFYPL
                     NLSGTPAVFPTPIPAEEQYSAAEVASFLNYIVEDGDTLESIAAKFNVSLEDLMRVNYI
                     SDMNVIETGWLIKIPGVPGPTQLTGEEGIVSISKYIKPDGRVRYQYTFLSSQSYNGIY
                     YELQGDNLEPLQNLVNRPVKIWGDISYSDTGTMFLDVKQFEEIYPGLQFQILQGKEAL
                     TEINGKPVALFTTNGTTYVMLSEVGAYQDISYKGDFPEDINIEALIVPGETYEGMPAL
                     RVFSMANAKDPNTGEAIELEPFAGEIQSSPDPFGNSDVYVEPDFIVDYIQLEYYVTDP
                     KMLYDAPTTDTLYIQPVWHFSGHYTNGTVSDILVQALKQEYLYPFNSP"
BASE COUNT          528 a          427 c          537 g          521 t
ORIGIN      
        1 atccaagccg aggcgttgga gatcgactac ggcaacagag tcagctgtcg ccttggaaag
       61 aagcgtaccg cctccgaggg gaacggtatt gggttgagtc agaagtgtca gggcttcgtc
      121 gagagtttga ggtctgtgat aggtcgagat catagaagtg cctccataga tatgcattat
      181 aaatcgttat ggagagggga gggtcatttt gaagcggact gcgagtagac gcatggtgaa
      241 aatggtgagc gcacctgcta acgatgcaag caaagtgttc acatgcagaa cataaagaat
      301 gataaataat gttgtgccaa cgaaagagca agtggcataa agctcggtac tctgaccgaa
      361 cactttgggg atgcggttgc tcaacacgtc acgtaagacg ccgccaaaga tgccagtgac
      421 cacgcccatg tgaatggcaa cgatgggaga aagattgagt tgtagggtat aggttgcgcc
      481 aagaatgcta aagatgccga gaccaatggc atcggctgtg gtgagcagta gttttgagat
      541 acggtcgagc cgatagaagg agataatagc gacgattaac gtagtgacgg ttaatgcagg
      601 gtcggcgatc cagaagatgg gcgtgcgccc cagcaataag tcgcggacgg taccgccgcc
      661 aaaggcgttg acataggcaa tggtcactgc gcctacaatg tccatttgat atttacgtgc
      721 gcctagcgct cctgaaaccg atgatgcaat gacggcgatg taagtgagaa tgagaagaag
      781 ttgatccatt cgccaagtat cacacaactt gtattaaaaa gtccaccatc atgtgatggt
      841 ggactttttt gttgctttac tggttacggt gaattgaagg ggtagagata ttcttgtttg
      901 agcgcttgga cgagaatatc agagacggtg ccgtttgtgt aatgtccgct aaagtgccag
      961 acgggttgaa tgtacaacgt gtctgtggtg ggagcgtcgt aaagcatctt ggggtcggta
     1021 acatagtatt cgagttggat gtaatcaacg atgaaatcag gttcgacata tacatctgag
     1081 ttcccgaatg gatctggaga tgattgtatc tcgccagcaa atggttctaa ttcaatagcc
     1141 tctccggtgt tcgggtcttt ggcatttgcc atactgaata ccctcaaggc gggcatgcct
     1201 tcgtatgttt cacccggcac gatcagagct tcaatgttga tatcttcagg gaaatcaccc
     1261 ttataactaa tatcttgata agcacctact tctgagagca tcacataggt ggtgccattg
     1321 gttgtaaaga gggcaacggg ttttccattg atctcggtaa gcgcttcttt gccttgcagt
     1381 atctggaatt gcaaccccgg gtagatttct tcgaactgtt ttacgtccaa gaacatcgtc
     1441 cctgtatctg agtagctgat atcgccccaa atcttaaccg ggcggttaac caagttttga
     1501 agtggctcaa gattatcgcc ttgcaattcg taatagatgc cattgtacga ctgtgagctt
     1561 aagaaggtgt attgataccg cactctgcca tctggtttga tgtacttcga gatgctcaca
     1621 attccttctt cgcctgttag ttgagtagga cctggcacgc ctggaatttt gatgagccag
     1681 cctgtttcaa tgacattcat atctgaaatg tagttcacgc gcatgaggtc ttccaacgag
     1741 acgttgaatt tggcagcgat gctttccaag gtgtcaccat cctctacaat atagttcaag
     1801 aaactagcga cttctgcggc gctatattgt tcttctgctg gaattggtgt tgggaaaaca
     1861 gcaggagtgc cgcttaagtt gagcggatag aaaccaaacc cgccgcctcc gccacctccg
     1921 cccatgctac tggcatctgc aacatattgg atgttcgacc agtctaaggt gttatcgacc
     1981 aaaattccag agataccgag gtatgactcc gta
//
LOCUS       DAOQJE010000732         2013 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_45494_length_2013_cov_1.722165, whole genome shotgun sequence.
ACCESSION   DAOQJE010000732 DAOQJE010000000
VERSION     DAOQJE010000732.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2013)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2013)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2013
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45494_length_2013_cov_1.722165"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(31..747)
                     /locus_tag="PLT08_15855"
     CDS             complement(31..747)
                     /locus_tag="PLT08_15855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE06001.1"
                     /translation="MNHKKENSLEPSMMDAILRDVYKYLFEEATLDNPARNQGLAREI
                     MVRYGSGISMEGSVFALIGEYLQSQPNPIALTQALGKAHDLFQLAFDFVSARGETEAG
                     YILLNIIVDAPSEDICAFAALTYASPRFVNEFAGRILEKRYKSIEGTSCKMAVALALL
                     QCGADRQIKSLIRDGFLEDYFSVSPGTSLEDKALRRGISSLYSRKILGILIEDIATEG
                     AGFSTPLNASPKTWKRKKYV"
     gene            complement(784..1485)
                     /locus_tag="PLT08_15860"
     CDS             complement(784..1485)
                     /locus_tag="PLT08_15860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HNE06002.1"
                     /translation="MTLTQATVDAIVRDLRKYFFEDAVSGNSARNQGLAREIIVRYGD
                     EVVIEGFLFATLGEFLTEQPNMLALTYALNAMPNLYNHAIDFVSARGQTGATVSLASI
                     VTTSTDEDVRAMAAITLALPEFSNSKLLKFIQDRLNQTEGTSCRMAIALLLYQCGDDR
                     ALKTFIRDGYFFDYFSTSKSMGRMSKGELQDTIAFMYRQEILKKIFEDIATNGRGFNG
                     PLGPSSKPWIRKQYD"
     gene            complement(1559..1939)
                     /locus_tag="PLT08_15865"
     CDS             complement(1559..1939)
                     /locus_tag="PLT08_15865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE06003.1"
                     /translation="MNEESVLFQRLSAYTNPLPFDFRQRVLAACETEGIPQFADPIIH
                     NLVGMRDVGIREGYGELHAGHIVAVVLSAIFMMIEQRYPAENVIAAIKVGPKLLVALI
                     PNPTVRKDALTYYFEKIDEIAKNK"
BASE COUNT          551 a          477 c          394 g          591 t
ORIGIN      
        1 cttatctgca taattacaga aaagtttatc ttacacatac tttttcctct tccacgtttt
       61 aggggaagcg ttcaaagggg tgctaaaccc tgccccttca gttgctatat cttcaatcaa
      121 tattccgaga atttttcttg aatacaaaga agatattccg cgccgtagtg ccttgtcttc
      181 caaactcgta ccgggactta cagaaaaata atcttcgaga aacccgtcgc gtatcaatga
      241 tttgatctgt cggtctgcgc cgcattgtag aagcgccaaa gcgaccgcca ttttgcaact
      301 ggttccttca atggatttat atcgtttctc taaaattctc ccagcaaact cgttgacaaa
      361 acgagggctg gcgtaggtca gggctgcaaa tgcgcatata tcttcagacg gagcatccac
      421 aataatattc agcaaaatgt aaccagcttc ggtctcaccc cgagcactta caaagtcgaa
      481 tgcaagctga aataggtcgt gggctttacc gagtgcttgt gtaagagcta ttggatttgg
      541 ttgtgactga agatattctc ctatcagtgc aaagacagac ccttccatgg aaattcctga
      601 tccatatcta accatgatct cccttgccaa accctgattg cgtgccgggt tgtcaagagt
      661 cgcctcttca aataaatatt tatagacatc gcgaagaatc gcatccatca tggatggttc
      721 aagggaattc tcttttttat gattcatttt tctgtttttg aagacacaac gaatcgacta
      781 gccttagtcg tattgtttcc gtatccacgg cttagaagac ggtcctaatg gaccattaaa
      841 tccccgcccg tttgtcgcaa tatcttcaaa tatttttttc aggatttctt gtctgtacat
      901 gaaggcgatt gtatcttgaa gttctccctt actcatccta cccatgcttt tactggtgga
      961 aaaataatca aagaaatacc catctctaat aaatgtcttt agggcacggt cgtcgccaca
     1021 ttgatacaaa agaagcgcga ttgccattcg acaactcgta ccttccgttt ggtttaatcg
     1081 atcctggata aacttcaata gtttactgtt tgagaattca ggtaaagcca acgtgatagc
     1141 tgccatagcc cggacatctt catcggtaga ggtcgtaact atggatgcta acgagacagt
     1201 tgcaccggtc tgaccacgcg cgctaacaaa atcaatggca tggttatata aattaggcat
     1261 ggcattcaac gcataggtta acgcaagcat attgggctgc tctgttaaaa actcacccaa
     1321 ggtggcaaat aaaaatccct caattaccac ctcgtctcca tagcgcacaa tgatttctct
     1381 cgccagcccc tggttgcgag ccgagttgcc ggagactgca tcctcaaaga aatatttcct
     1441 taagtcccga acgattgcat cgacggttgc ctgagttagt gtcatatcca tttcctctct
     1501 atttagatca gattgaatga actgtgcagc ggacaaatct ctctgccgct gtcaaaaatt
     1561 acttgttttt tgcaatctca tctatcttct caaaataata tgtaagcgcg tcttttcgta
     1621 cagttgggtt ggggatgagt gcaactaaca atttgggtcc gactttgatg gcggcaatca
     1681 cattttctgc tggatatctt tgctctatca tcatgaaaat tgctgataac acgaccgcaa
     1741 caatatgccc ggcatgtaac tcgccatacc cctctcggat acctacgtcc ctcattccca
     1801 ctagattatg gatgattgga tcagcaaatt gtggaattcc ctctgtctca caagcagcca
     1861 ataccctttg acggaaatca aaaggtaatg ggtttgtata ggcagacagt ctttgaaaca
     1921 atacagactc ttcgttcata gcggctcctt tgcaaaccat ctagcaggaa aaaccctatt
     1981 acgtctggta atactgtcta attattttgg cag
//
LOCUS       DAOQJE010000733         2011 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_45545_length_2011_cov_7.164622, whole genome shotgun sequence.
ACCESSION   DAOQJE010000733 DAOQJE010000000
VERSION     DAOQJE010000733.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2011)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2011)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2011
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45545_length_2011_cov_7.164622"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(48..497)
                     /gene="nrdR"
                     /locus_tag="PLT08_15870"
     CDS             complement(48..497)
                     /gene="nrdR"
                     /locus_tag="PLT08_15870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
                     /protein_id="HNE06004.1"
                     /translation="MRCPYCKHHDSKVLDTSHDSHGGIRRRRECLKCRQRFSSYERPI
                     LATPLIIKQDGNREEFDREKMARGIRISCAKRPVSAADIERMIGQVEVKLQKLGKAEI
                     SSRVVGDLVMETLKEVDQVAYIRYAIVYLGLDDLKSIRTEIDQLLED"
     gene            complement(828..1685)
                     /locus_tag="PLT08_15875"
     CDS             complement(828..1685)
                     /locus_tag="PLT08_15875"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HNE06005.1"
                     /translation="MAVQTKNQIEYKTIRLSNGRKVNYVHQGAGEPVVMVHGLAASLH
                     DWDDLLPEVSAAGYAGYALDLLGHGESEKPSHTHDYSVENAYADFSAWIDSLNISFPI
                     NLIGHSLGGGLSLLYTYRNPEKVKSLVLVNPFYSMKQLPQILQKMFHHQLINTTLIDR
                     TPYRVFRFFVDMTSFSTYIGSRETHVLPEHIRYQTALDYKRAASGIYNMPRTLHHLDL
                     DLARITQPTLLLWGGRDQTLAPSSFPQLAEKLPNLVKKHSFPICGHVPHQCHPADFNP
                     YVMEFLDKN"
     gene            complement(1799..>2011)
                     /locus_tag="PLT08_15880"
     CDS             complement(1799..>2011)
                     /locus_tag="PLT08_15880"
                     /inference="COORDINATES: protein motif:HMM:NF024591.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE06006.1"
                     /translation="VAVAGSPFAFVIAGKEKPSPEAVIAYCKRRLPPQLVPRFVIFME
                     EFPRTFIGKVLRRELAKRYGNKIASE"
BASE COUNT          522 a          514 c          470 g          505 t
ORIGIN      
        1 gatacaaaaa ggcaaagaca ctccccccaa agagggtcct tgcctaatta atcttcgagt
       61 aactggtcga tctcagtacg aatggacttg aggtcgtcca agcccaaata cacaatcgca
      121 tagcggatgt aagccacctg gtccacctct ttcaaggtct ccatcaccaa gtcacctacc
      181 acgcgcgaag aaatctccgc tttgcctaat ttctgcaatt tcacttccac ctgcccgatc
      241 atgcgctcaa tgtccgccgc agaaacgggt cgcttcgcac aggaaatgcg aatgccacgc
      301 gccatcttct cgcggtcaaa ctcttcgcgg ttgccgtctt gcttaataat gagcggggtt
      361 gccaaaatgg gtcgttcgta gctgctgaag cgctggcggc actttaggca ctcccgtcgt
      421 cggcgaatcc cgccgtgact gtcgtgtgag gtgtccagca ccttcgagtc atgatgttta
      481 caatacggac aacgcatccg gtagaagctc caatttagaa caaatgattt agccatatat
      541 gggcgtaaaa gcgctgcatc cccgtatatc tggctaatgt gttcggcatt ttagcaccga
      601 ggtgaattgt gtgcaagagg gtatttgaaa actgcaatct taaaatattg actcgattcc
      661 ccattaggcg ctagatgatg gacaaaaatc cgcgcttcat tcttaacgtt gaattgattc
      721 tcccgctgca aacattccta cactgaacgt tttttctcag aaaaacaaaa acccgaagag
      781 ctaaaagtat atttactctt cgggtttatt tattctatcg ttcaatgtca gttcttatcc
      841 aagaactcca tcacgtacgg gttgaaatcc gctgggtgac attgatgcgg cacatgtcca
      901 cagatcggga aggaatgttt cttcaccaag ttcggcaact tctctgccaa ctgcgggaaa
      961 gaagacgggg caagcgtttg gtcacgtccg ccccacagca gcagagtcgg ctgggtgatg
     1021 cgcgccaagt ccaggtcaag gtgatgaagc gtgcgcggca tattgtaaat gcccgaagcc
     1081 gcgcgtttgt aatccagcgc ggtctgataa cgaatatgtt ccggcaacac gtgcgtctcg
     1141 cggctgccaa tataggtgct aaagctggtc atatctacaa aaaagcggaa gacgcgatac
     1201 ggcgtacggt cgatcaaggt cgtgttgatc aattgatgat ggaacatctt ctgcagaatc
     1261 tgcggcaact gtttcatcga atagaacggg ttgaccaaca ccaaagactt aaccttctca
     1321 gggttgcgat aggtgtataa cagcgaaagc ccgccaccca acgaatgccc aatcaaatta
     1381 atcgggaagg aaatattcag ggagtcgatc catgctgaga aatctgcata ggcgttttcc
     1441 actgaataat cgtgcgtatg gcttggcttt tcgctttcgc catgcccaag aagatccagt
     1501 gcatacccgg catagcccgc tgcagaaact tctggcagca ggtcatccca atcatgcaaa
     1561 gatgccgcca acccatgcac cataacgaca ggttcccctg ccccctgatg tacataattc
     1621 actttgcgtc catttgataa acgtatagtt ttatattcga tttgattctt cgtctgtact
     1681 gccatgtgta tctcacaaag atgccgcatg tgtaggacgc ggcatctgta aaacatctta
     1741 attcaattgt attaatatcg ggtaaacatt tacccgtcaa aatcgttagt cttggtttct
     1801 attcgctggc gatcttattc ccgtagcgtt tggcgagttc acgtcgcagc actttaccga
     1861 taaaggttct ggggaattct tccataaaga tcacaaaacg cggcacaagc tgcggcggca
     1921 aacggcgttt gcaatatgcg atcacggctt cagggctggg cttttctttg ccagcgatca
     1981 cgaacgcaaa ggggctgccc gcaactgcta c
//
LOCUS       DAOQJE010000734         2010 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_45591_length_2010_cov_5.153964, whole genome shotgun sequence.
ACCESSION   DAOQJE010000734 DAOQJE010000000
VERSION     DAOQJE010000734.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2010)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2010)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2010
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45591_length_2010_cov_5.153964"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..541
                     /locus_tag="PLT08_15885"
     CDS             <1..541
                     /locus_tag="PLT08_15885"
                     /inference="COORDINATES: protein motif:HMM:NF024591.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE06007.1"
                     /translation="VIKAPWIGMTRGFWKDQQRYLDTYWSRWENVWVHGDFAAVDNDG
                     LWYILGRSDDTIKIAGKRLGPAEVESILVRHESIVEAAAIGVPHEVKGSELVLFAVTK
                     PGVERSAVLRQELQEMVVAEMGKPLAPKAILFVNDLPKTRNAKVMRRMIRAAYLGLEL
                     GDTSSLVNPQAVEEIRKSL"
     gene            695..1747
                     /locus_tag="PLT08_15890"
     CDS             695..1747
                     /locus_tag="PLT08_15890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE06008.1"
                     /translation="MRNNFRGLLFVFLSLVLMMMACNLGAAAPTEIVSEPPTEALSAA
                     TEAPVTAEVQHVTIPGELPDKQSGLAGDQDSSTTADEKRAPAGDRFTYGRYERPFNAN
                     EMDVYFSNIDILVSEVYQDDVWVYGTLTLKDTGSGCSLDGKYGFEIDTNIDGGGDLLV
                     LVTKPTSTNWSTDGVEVWFDENDDVGGTKKVFSDESSSVGNGYESQLFGAGAGNDPDL
                     AWARIAPTDPCMVQMAVKQAGLTGPRYMIGMWAGNDLLNPALFDHNDGFTHDQAGSSL
                     IEFEYFYPVKEVYELDNVCRMAVGFDPTGSEPGICPVPPSGGGDEPPPPPGNSCPPPS
                     ILFCGRNGCYCLEPAG"
BASE COUNT          503 a          384 c          542 g          581 t
ORIGIN      
        1 ggtaataaaa gccccgtgga ttgggatgac gcgcggcttt tggaaagatc aacagcgcta
       61 tttagatacg tactggagcc gctgggaaaa tgtttgggtg cacggtgatt ttgcagcagt
      121 ggataatgac ggcttgtggt atatccttgg gcgttcagat gacacgatca aaattgctgg
      181 taagcggctc ggaccggctg aggtggagtc gattcttgtg cggcatgagt ccattgtgga
      241 ggcggcggca attggggtgc cacacgaggt caaggggagt gaactggtcc ttttcgcagt
      301 gaccaagccc ggggtagagc gaagcgcggt tcttcgtcag gaattgcaag aaatggtagt
      361 tgcagagatg ggtaaaccgc ttgccccgaa agctatcctg tttgtgaatg atttacccaa
      421 aacgcgcaat gctaaggtaa tgcgccgaat gattcgcgcg gcatatttgg ggttggagtt
      481 gggggatact tcttcgttag ttaatccgca ggcggtggaa gagatccgaa aatctttata
      541 gaaaactcaa tgcatttcaa aaggcgagga taacatcctc gccttttata ttttaatgac
      601 ataagtacta gctttgcttt taggtgagac tattttccta ttgttttata ccattatcaa
      661 actgtatagt ctgggtaaga aaggaaaggt ttttatgaga aataacttta gaggtttatt
      721 gttcgtgttt ttgtctttag tgcttatgat gatggcatgt aatttaggtg ccgctgcgcc
      781 tactgaaatt gtaagtgagc cgcccacaga agctttatcc gcggcaacag aagcgcctgt
      841 tactgcggaa gttcagcatg tgacgattcc cggtgaattg ccggataaac aaagtggttt
      901 ggctggtgat caggactcat ccacaactgc ggatgaaaag cgtgccccgg ctggagatcg
      961 tttcacttat gggcgttatg agcgtccatt caatgccaat gaaatggatg tgtatttttc
     1021 taacattgac attcttgtga gcgaggtcta tcaggatgat gtgtgggttt atggaacctt
     1081 gaccctcaag gatactggtt ctggttgtag cttggacggc aagtatgggt ttgaaattga
     1141 taccaatatc gatggcggcg gtgatttgct ggtattggtt accaagccta catctactaa
     1201 ctggagcacg gatggtgtgg aggtttggtt cgatgagaat gatgatgttg gcggcaccaa
     1261 aaaagtattt tctgatgaaa gctcatcggt aggtaatgga tatgaaagtc agttattcgg
     1321 tgctggggca gggaatgacc cagaccttgc ttgggcgcgc attgctccaa cagacccttg
     1381 tatggtgcaa atggcagtta agcaggctgg gctgaccgga cctcgttaca tgatcggaat
     1441 gtgggctggg aatgatttgt taaacccagc attgttcgac cataacgacg gcttcacaca
     1501 cgaccaagcc ggctcttcat tgatcgaatt cgaatatttt tacccggtca aagaagtgta
     1561 cgaattggat aatgtgtgtc gtatggctgt tggttttgac cctaccggtt ctgaaccagg
     1621 aatttgccct gtgccgccat ctggcggtgg agatgaacct ccgcctcctc ctgggaatag
     1681 ttgtcctccg ccttccattt tattctgtgg tcgaaatggc tgttattgtt tggaacctgc
     1741 cggatagatt tagagtagtt gtttaacaaa agtatggctg atagttcttt gtcagccata
     1801 cttttgtttt aatgaataaa taacaaagag tggtactttt acaattcatt ctttcgggtt
     1861 tcccctactt cgcaatgata ttgcaatgaa atatagtgta ggcaagcaag tgacaaaact
     1921 tcgctcacac tataacaagg agaaaatcat gaaacgtttc agcactgtcg ctttggttct
     1981 tttgacttta cttgccctaa caacgggaac
//
LOCUS       DAOQJE010000735         1992 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_46430_length_1992_cov_2.701084, whole genome shotgun sequence.
ACCESSION   DAOQJE010000735 DAOQJE010000000
VERSION     DAOQJE010000735.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1992)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1992)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1992
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46430_length_1992_cov_2.701084"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..286)
                     /locus_tag="PLT08_15895"
     CDS             complement(<1..286)
                     /locus_tag="PLT08_15895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE06009.1"
                     /translation="MTKMQKPDYFDEIQQNALILWRQLEYNRELAAPWHQLFKQVQSP
                     RHVLSELLQNADDAKATEVKVQIKGGVFSFVHNGKDFNKDDFASLCRFGYS"
     gene            complement(289..>1992)
                     /locus_tag="PLT08_15900"
     CDS             complement(289..>1992)
                     /locus_tag="PLT08_15900"
                     /inference="COORDINATES: protein motif:HMM:NF024420.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3883 domain-containing protein"
                     /protein_id="HNE06010.1"
                     /translation="RFGRIHRIGQTEVCHLWNLVSDQTREGEVYIRLLKKMEEQRKAL
                     GDGVFDVLGKLFRDTSLRQLLMEAIRYGNKPEVKARLFQAVDNLADRERVRELLEERS
                     LAQDTMDVSHVHHIREDFERAQAKRLQPHFIAGFFKAAFEHLGGSIHSREPLRYEITH
                     VPAPIRNRAGQMGRGAVLPKYERVTFHKEAIQQPGKATAEFIAPGNPLMDAVTDLILE
                     RYRGLLRQGTILVDPQDQGIEPRILYYLEHAIHDSRSTQSGERRTISKQMQFVELNSK
                     GEAHSAGSAPFLDYRPLEDEHKSVVDTILDKDWLRGDLEAHIRAYAAQNLVPQHLQEV
                     KSHREESIAKTESAVRDRLTKEINYWDYRAEELKAQEQAGRTNARLNSELARRRADEL
                     QARLQKRMEELEQERHISAAPPVLMGGALVIPAGLLNKLKNNPPSLFARQTKAVEDAG
                     MQAVMDKERKLGFIPKDVSREKLGWDIESQVGEGKLRFIEVKGRIEGSTTVTVTRKEI
                     LAALNKPDDFILAVVEVTFDGEKAIGKEPTYIKKPFQREPDFGAVSVNYELAELLSNA
                     Q"
BASE COUNT          458 a          499 c          461 g          574 t
ORIGIN      
        1 tagaatatcc aaagcgacaa agtgaagcaa aatcatcctt gttgaaatcc ttgccattat
       61 gtacaaagga gaaaacccca ccctttattt gtaccttgac ctcggtcgcc ttggcatcat
      121 ctgcattttg aagaagttct gagagaacat ggcgaggact ttgtacttgc ttgaataatt
      181 gatgccaggg tgcagccaac tcgcggttgt attcaagttg ccgccagagg atgagagcgt
      241 tttgttggat ttcatcaaaa taatcaggct tctgcatttt tgtcatggct attgggcgtt
      301 tgatagcaac tcagctaact cataattaac actgacagct ccaaagtctg gctcgcgctg
      361 aaagggtttt ttgatgtaag tgggttcctt gccaatcgct ttttcaccat cgaaggtcac
      421 ttccaccaca gctagaataa aatcatcagg cttgttaagc gccgcaagga tctccttacg
      481 tgtcactgtt acggtggttg agccctctat gcgccctttc acttcgatga agcgcaattt
      541 cccttctcca acctgggatt cgatgtccca gccaagtttc tcgcgactaa catccttggg
      601 aataaaccct agcttgcgct ctttatccat cacggcttgc atgccggcgt cttccacggc
      661 tttcgtctgt cgtgcaaata gactcggtgg attatttttc agtttattta gcaaacctgc
      721 aggaatgacc aacgcgccac ccataagaac cggaggagcc gctgaaatat gccgctcctg
      781 ttccagctct tccatgcgct tttgcaggcg ggcttgtaat tcatctgcgc ggcgtcgtgc
      841 cagttccgaa tttaggcgcg cattggttct gccagcttgt tcctgggctt tcaattcttc
      901 tgcacgataa tcccaataat tgatttcttt cgtaaggcgg tcacgaacgg cagattcagt
      961 tttcgcaata ctttcttcac gatgggattt cacttcttga aggtgttgtg gtacaaggtt
     1021 ttgtgcggcg tatgcccgaa tgtgtgcttc taaatctcct cgtaaccagt ctttatccaa
     1081 aatggtatca acgacagatt tatgttcgtc ttccagtggc cgatagtcca aaaacggggc
     1141 ggatcccgcc gaatgcgcct cacctttcga attgagctca acaaattgca tttgtttcga
     1201 gatagtcctg cgctcgccac tctgtgtgga ccgtgagtca tggattgcat gttcaagata
     1261 atacaatatc cggggctcaa taccctgatc ctgcggatcc accaatattg ttccctgacg
     1321 caacaagcca cgataacgtt ctagaattaa gtcagttaca gcatccatta gtggattgcc
     1381 tggggcgatg aattccgctg tcgccttgcc tggctgttga atagcttctt tgtggaaggt
     1441 gacgcgttca tatttaggca atacagcccc tcgacccatt tgccctgctc ggtttcggat
     1501 tggtgctggc acatgcgtta tctcgtagcg taacggctca cgggagtgga tactgccacc
     1561 taaatgttca aatgcagctt tgaaaaagcc tgcaataaag tgtggttgta gtctttttgc
     1621 ctgcgcccgt tcgaaatctt ctcgaatatg atggacatgg ctgacatcca tcgtatcctg
     1681 ggcaagggag cgttcttcca gtagttcacg aacgcgctct ctgtctgcaa gattgtccac
     1741 tgcttggaat aggcgagcct ttacttcggg tttgttccca tagcggatag cttccattag
     1801 taattggcgg aggctggtat cacggaataa tttaccaagt acgtcaaaaa ccccatcgcc
     1861 caacgctttg cgctgctcct ccatcttttt aagaagccga atatatactt cgccttcgcg
     1921 tgtctggtcg gataccaaat tccacagatg gcagacttcg gtctggccga tacggtgaat
     1981 acggccgaaa cg
//
LOCUS       DAOQJE010000736         1991 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_46446_length_1991_cov_5.432851, whole genome shotgun sequence.
ACCESSION   DAOQJE010000736 DAOQJE010000000
VERSION     DAOQJE010000736.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1991)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1991)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1991
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46446_length_1991_cov_5.432851"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..900
                     /locus_tag="PLT08_15905"
     CDS             <1..900
                     /locus_tag="PLT08_15905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HNE06011.1"
                     /translation="YTEAIMQDTLPQSAVIVGAGAIGVEFATIWNSYGVDVTIVELLP
                     RVLPREDEEISKELTKELTKNGIKIKTGIKFESISVSGNKVKVVLPDGTLEVDQVLVA
                     TSFTPNSKGLGLEAVGVKISDRGFIEINEKMQTNIPSIWAIGDVTGKLMLAHVGSAMG
                     IVAAEHIGGHETITLNYEMMPRATYCHPQVASFGLTEAQAKERGYNIKVGRFPFQANG
                     KALGLGDYAGFVKIITDEKYGEILGAHMMGPEVTELLPELTLAQMMELTPHEIARNVH
                     AHPTLSETLMEAAHGASGTPIHI"
     gene            complement(945..1874)
                     /locus_tag="PLT08_15910"
     CDS             complement(945..1874)
                     /locus_tag="PLT08_15910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE06012.1"
                     /translation="MVRKLSVVLLILFQISCSAATPEPTATLTPLPPTATFTATPAPT
                     ATQPPASPMPDAITYEGNGFRFDVPDTLDVDMQRNVVGIRDKSGTLIISFTRVPYDES
                     TDTLEDVINDYLAEISTRGGGKFTQSEATSITVAGAEGLSVELSGTLFERPIEGRAIA
                     VRPDKNSVLFGMGVSNLVSDETLWQSTGSLIFQGLLDSLEFVDVQIAAQCTVSTDKSY
                     GYTETNPIKVGGDFIDGPARERAYLDNLLGPNGEALSYEREGSINSGDTILDIYRVTG
                     NGLNVTLYLDEYTYSPLLAPVGLTCVNEFISAP"
BASE COUNT          547 a          502 c          475 g          467 t
ORIGIN      
        1 tacaccgaag ctattatgca agataccctc cctcagtctg cggtgatcgt gggtgcgggt
       61 gccatcggcg tagaatttgc caccatttgg aattcctacg gtgtggacgt gaccatcgtg
      121 gaactgcttc cgcgtgtgtt accacgtgaa gatgaagaaa tcagcaagga actcaccaaa
      181 gagctgacca agaacggcat caagatcaaa actggcatca agtttgaaag catctccgtc
      241 agcgggaaca aagtgaaagt tgtgttgccc gatggaacgt tagaagtgga ccaggtcttg
      301 gtcgcaactt cctttacgcc gaacagcaaa gggctggggc ttgaggcggt tggtgtcaag
      361 atcagcgacc gtggcttcat tgagatcaat gaaaagatgc agaccaacat ccccagcatt
      421 tgggcaattg gcgacgtgac cggcaaactc atgctggcac atgtcggctc agcgatgggc
      481 attgtggctg ctgaacacat tggcgggcat gaaaccatca ccctcaatta cgagatgatg
      541 ccacgtgcta cctactgtca tccgcaggtc gcttcgtttg ggctgaccga agcgcaagca
      601 aaagagcgcg gatacaacat caaggttggg cgcttcccct tccaggcaaa tggcaaggca
      661 cttgggctgg gtgattatgc aggcttcgtc aagatcatca ccgatgaaaa atatggcgag
      721 atcctcggtg ctcatatgat gggacccgaa gtgaccgaac tgttgcccga actgaccctc
      781 gcgcaaatga tggaactgac tccgcacgag attgctcgta acgttcatgc gcacccaacg
      841 ctttcagaaa cccttatgga agccgcacac ggcgcaagcg gaactcccat tcacatctaa
      901 aacaaaactc cgaagcttca tcgcttcgga gttttttatt cacattacgg ggcagagata
      961 aactcattca cacaggtcaa ccccaccggt gcaaggagag gtgaatatgt gtattcatcc
     1021 aaatacaagg ttacattcaa accgttgcct gtaacacgat aaatatccaa gatcgtgtca
     1081 ccgctattta tagagccttc acgttcataa gacaaagcct caccattcgg acccaacaaa
     1141 ttatcgagat aggctcgttc acgagcaggt ccatcgataa aatcgcctcc gaccttgatc
     1201 gggttggttt ctgtataccc atagctttta tctgtgctga cagtacactg cgcagcgatt
     1261 tgcacatcga caaattccag tgaatccaat agcccctgaa agatcaatga accagtgctt
     1321 tgccatagcg tctcatcact gactaaattt gagactccca taccaaatag caccgaattc
     1381 ttgtcaggtc tcaccgcaat ggctctgcct tcaatgggtc tctcaaaaag agtgccgctt
     1441 agttccacag aaagcccctc tgccccagcc actgtgatcg aagtcgcctc gctttgggtg
     1501 aacttgccgc caccacgcgt agaaatctct gccagataat cattgatcac atcttccaat
     1561 gtatcggtag attcatcata tggcacccgg gtaaatgaga tgattagggt gccagattta
     1621 tcacgaatcc ccacaacatt cctttgcata tccacgtcca aagtatcggg gacatcgaag
     1681 cggaacccat taccctcgta tgtgatcgca tcaggcatgg ggctggcagg aggctgcgtg
     1741 gcggtcggag caggtgtagc tgtgaatgta gccgttggag gcaatggagt tagagtcgcc
     1801 gtcggttcgg gggtcgctgc actgcaagaa atttggaaca gaatcaacaa aaccacagaa
     1861 agttttcgaa ccatttagaa tcctcctttt ttggtaaagg gaaaggtaca agccttatca
     1921 aaatataacc gaaatctata gaaatctcca tatttgtata tacatcttga aatttttaag
     1981 attgtatgct a
//
LOCUS       DAOQJE010000737         1991 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_46454_length_1991_cov_3.368285, whole genome shotgun sequence.
ACCESSION   DAOQJE010000737 DAOQJE010000000
VERSION     DAOQJE010000737.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1991)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1991)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1991
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46454_length_1991_cov_3.368285"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1012
                     /locus_tag="PLT08_15915"
     CDS             <1..1012
                     /locus_tag="PLT08_15915"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HNE06013.1"
                     /translation="NEFAHGGAVKLTYLAETFPHHFPTANALYAVSSVAHPLQLEILE
                     TAKKKELKIVVNQNGVAFPAWDGDNYEVSNRSLRQLISYADFIIYQSQFCKDSAERYI
                     SPPDVPNEIIYNPVDTRLFSPEACSAKPAALTLLLGGNQYEKYRLELALQTLKALHHN
                     VPNAKLIVTGNLWLPRDEAEAWTKQALQDMNLTEHVIFTGTYAQTDAPALYSQAHLLL
                     HTKYADPSPGLVLEAMALGMPVVHLDNGGVPELVGNAGIGVHVEHDWNKINLPNPQAM
                     ADAVMRVYDRHNEFSEKARQRAVDLFSLEKFIARHKEIFEKVLGDSHLLHHENLRYHL
                     HP"
     gene            981..1871
                     /locus_tag="PLT08_15920"
     CDS             981..1871
                     /locus_tag="PLT08_15920"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNE06014.1"
                     /translation="MKISVIICTRNRFDDFTKTLPSIAAQTRLPDELIIVDSSDEKKL
                     EEYFSTTKLPFFVRYFHTQPGLTLQRNYGIRECVSDIIFFFDDDVNLDKNYLAEVEKI
                     FVNDTSHKIGAVGGKIIEKSSSSVWLKFEQFTFYVLRLVFGVVGTRNGKFYPSGMPSH
                     PHTNQPSGFIECLSGCCMAFRREVFEKAKFDEALAHYGLMEDVDISKQTLDAGYKIYY
                     QTSATLIHNESPMNRLKVQQWAEMSVVNYDYLFRKSWAQEKWRWLFYYWALIGLFVAN
                     FHSLKGLTGTFNGVKKVFFK"
BASE COUNT          528 a          461 c          474 g          528 t
ORIGIN      
        1 taatgaattt gcgcatggcg gcgcggtcaa gttgacctat cttgccgaaa cctttccgca
       61 tcattttcca actgccaatg cgctttatgc agtcagtagt gtggctcatc ctttacaatt
      121 ggaaattctt gaaacagcga aaaaaaagga actaaaaatc gttgtcaacc agaacggagt
      181 tgctttccct gcatgggacg gtgataatta cgaggtatca aaccgttcgt tgaggcagtt
      241 gatcagctat gcggatttca tcatttatca aagtcaattt tgcaaagata gtgcggagcg
      301 atatatctcc ccgccagatg tgcccaatga gatcatttac aacccggtgg atacccgcct
      361 cttctcaccc gaagcctgct cggcaaaacc tgcggcattg actttgcttt tgggcggcaa
      421 ccaatacgaa aagtatcgcc ttgaactggc gctgcaaacg ctcaaggcac tacaccataa
      481 tgtgcctaat gcgaagctaa tagtaactgg caatctctgg ctcccacgcg acgaagccga
      541 agcatggacg aagcaagccc tgcaagatat gaacctgacg gagcatgtaa tctttaccgg
      601 aacttatgct caaacagacg ctccagcctt gtattcccaa gctcatctct tgctgcacac
      661 taaatatgct gacccatcac cggggctggt cttggaggcg atggcgttgg gaatgccggt
      721 cgtccatttg gacaatggtg gtgtgccgga gttggttggc aatgcaggca ttggtgttca
      781 tgtggaacac gattggaaca agatcaacct gccaaatcca caagccatgg cagatgcagt
      841 gatgcgggtc tatgatcgcc acaacgaatt ttccgaaaaa gcacgtcaac gggcagtgga
      901 tttattttcg ttggaaaagt tcatagcaag gcataaagaa atcttcgaaa aggtgttagg
      961 tgacagtcac ctcttgcatc atgaaaatct ccgttatcat ctgcacccgt aatcgcttcg
     1021 acgattttac gaagaccctg ccatccatag cagcgcaaac ccgcctgcca gatgagttga
     1081 tcatcgtcga ttcgtcggat gagaaaaagc tggaagagta tttcagtaca actaaactgc
     1141 ctttctttgt gcgttatttt catacccagc ccggcttgac cttgcagcgc aattacggca
     1201 tccgcgagtg tgtgagtgac atcatctttt tctttgacga cgatgtcaat ttggacaaga
     1261 actatcttgc cgaagtagaa aaaatttttg taaacgatac aagtcataag atcggcgcag
     1321 ttggtggaaa gattattgaa aaatcatcct catctgtgtg gcttaaattt gagcaattca
     1381 cgttttacgt tttacgcctt gtcttcggtg tggttggaac ccgcaatggg aaattctatc
     1441 cttctggcat gccatcgcac ccacacacaa accagccaag cggattcatc gaatgtctct
     1501 ctggctgttg catggcattt cggcgtgaag tgtttgagaa agccaaattc gatgaagcac
     1561 ttgcccatta cggtctgatg gaagatgtgg atatttccaa acagactctg gatgctgggt
     1621 ataaaatcta ctatcaaact tctgccactc tcatccacaa cgaaagcccg atgaaccgcc
     1681 tcaaagtgca gcaatgggca gagatgagtg tggtcaatta tgattacctc tttcgcaaga
     1741 gttgggcgca ggagaaatgg cgttggcttt tttattattg ggctttgatt ggtttgtttg
     1801 tggcgaattt tcacagcctc aaaggcttga ccggcacatt caacggtgtg aagaaagttt
     1861 ttttcaaatg aaacgcacac tcattacagg catttctggt tttgttggca agcaccttcg
     1921 cgatgaactc gtccgtgaag gctgggatgt ttatggattc gacatccgcc cggcgggaga
     1981 gaatgtcttc g
//
LOCUS       DAOQJE010000738         1990 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_46487_length_1990_cov_6.186563, whole genome shotgun sequence.
ACCESSION   DAOQJE010000738 DAOQJE010000000
VERSION     DAOQJE010000738.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1990)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1990)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1990
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46487_length_1990_cov_6.186563"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..442)
                     /locus_tag="PLT08_15925"
     CDS             complement(<1..442)
                     /locus_tag="PLT08_15925"
                     /inference="COORDINATES: protein motif:HMM:NF024976.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction enzyme HsdR N-terminal
                     domain-containing protein"
                     /protein_id="HNE06015.1"
                     /translation="MTSQTTAPAIIHKLVETFEQNLDSYRSSKNETELRREFLDKFFT
                     ALGWDVANEKGYDELGKEVIHEFSVDVAGQQKKADYAFRVGRGEKFDFLVEAKKPSVK
                     IESSPEAAFQIRRYGWSAKLPINILSDFEHFAVYDCRTKPSYNDK"
     gene            complement(624..1601)
                     /locus_tag="PLT08_15930"
     CDS             complement(624..1601)
                     /locus_tag="PLT08_15930"
                     /inference="COORDINATES: protein motif:HMM:NF024197.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EcsC family protein"
                     /protein_id="HNE06016.1"
                     /translation="MRPTKRAPDVWDSAAFSSIFLASSFFCSQALSTPAHTQVTQTVR
                     RMTTTHYKQDWDEIMTNNDEQEFTEYELNDLRTAKALLEYPSLTAKIADLVGTPIETG
                     FKYLPKNWNQKIGEITQSALLKGLEFSILTMGKPETKESQDWLHKLLVMGSGAAGGAV
                     GLASMSVELPFSTCVILRSIADIARSEEHNIALLETKLECLQVLALGGKNAKDDASES
                     GYWMVRGALAKSVSEATVYIAEKGLAEEGAPPLIKLITTIASRFSVVITEEAAAKTIP
                     IVGAISGGAINYLFMNHFQGMARGHFMVKRLEKKYGMKHVEKTYKELVI"
     gene            complement(1620..1841)
                     /gene="merB"
                     /locus_tag="PLT08_15935"
     CDS             complement(1620..1841)
                     /gene="merB"
                     /locus_tag="PLT08_15935"
                     /EC_number="4.99.1.2"
                     /inference="COORDINATES: protein motif:HMM:NF015216.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018836 - alkylmercury lyase activity
                     [Evidence IEA];
                     GO_process: GO:0046413 - organomercury catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="organomercurial lyase"
                     /protein_id="HNE06017.1"
                     /translation="MLFRSKEHAETWCGQTQMPAGQLLSFEQVWELSKLWYQDRLLPE
                     FKGRSIAEAHEIFEKLGLNSDFWRFDISS"
BASE COUNT          543 a          448 c          396 g          603 t
ORIGIN      
        1 ccttgtcgtt ataacttggc tttgttcggc aatcataaac ggcgaaatgc tcaaagtcgg
       61 acaaaatatt aatgggaagt tttgcgctcc acccataacg gcggatttga aaagccgctt
      121 ctgggctgga ttcaattttt accgatggct tttttgcttc aaccagaaaa tcgaactttt
      181 cccctcgccc aacacgaaaa gcataatcgg cttttttctg ctgacctgca acatctaccg
      241 aaaattcgtg tatgacttcc ttccctaatt catcgtaacc tttttcattt gcaacatccc
      301 aacccaacgc agtaaaaaac ttatccaaaa attcacggcg caattcggtt tcgttcttgc
      361 tggaacgata cgaatctaaa ttctgctcga aagtctcaac caatttatga ataattgcgg
      421 gggcggtagt ttgtgatgtc attccctgat tttacctgat agaaaaaata tattgttttg
      481 cacgaagggg tctaacggtt tgcattaccc gcgtgtgggc gagcgtggac tctgcttggg
      541 agcaggaaaa actcgaagcg agaaaaatgc tcgaaggtgc cgcagaatcc cacacgtcag
      601 gtgcatgctt tgttaggcgg gtcttatatt accaactctt tatatgtttt ttctacatgt
      661 ttcatgccgt attttttttc aaggcgtttt accataaagt gacctcgtgc cattccttga
      721 aagtgattca tgaataaata attgattgct cctcccgata tagcgccaac aattggaatg
      781 gttttggctg ccgcttcttc agtaataaca acactaaatc tagaggcaat tgtagtaata
      841 agttttatca gaggcggagc gccttcctcg gctaatccct tttccgcaat ataaactgtt
      901 gcttctgata cagattttgc caatgctccg cgcaccatcc agtaaccgct ttcagaagcg
      961 tcatctttcg cgttttttcc tccaagagcg agaacctgca aacattctaa ttttgtttcc
     1021 aataaagcga tattatgttc ttcactgcgg gcaatatcgg cgattgagcg cagaattaca
     1081 caagtggaaa aaggtaattc aacagacatt gaggcaagcc caacagcgcc acctgccgct
     1141 ccagaaccca taaccaatag tttgtgtagc cagtcttgag actcttttgt ctcaggtttc
     1201 cccatagtaa gtatggaaaa ctccagccct ttaagtagtg ctgactgagt aatttcgcca
     1261 attttctgat tccaattctt tgggagatat ttgaaaccag tttcaattgg tgtaccaact
     1321 aagtctgcaa ttttagcagt tagactggga tactcaagaa gggctttcgc cgttctgagg
     1381 tcatttaatt catattcagt aaattcttgt tcgtcattat ttgtcataat ttcatcccaa
     1441 tcttgcttgt agtgtgttgt tgtcatccgc ctaacggttt gcgttacctg cgtgtgggcg
     1501 ggcgtggaca atgcttggga gcagaaaaaa ctcgaagcca gaaaaatgct tgaaaatgcc
     1561 gcagaatccc acacgtcagg tgcacgcttt gttgggcgca ttttaccaga accacgtttt
     1621 taacttgaaa tatcaaaccg ccaaaaatca ctattcaaac caagtttttc aaatatttcg
     1681 tgcgcttctg caattgagcg tcctttgaac tcaggcaaga gacggtcttg ataccataat
     1741 ttagaaagtt cccaaacttg ctcaaaagat aataattgtc cagcgggcat ttgcgtttga
     1801 ccgcaccaag tttctgcatg ttctttcgac cgaaagagca aaatatttgc tcaggtaaaa
     1861 attaagtcat cgtaccattg accacaaggc aagggaaaat aaaccatgtg gttttttccg
     1921 tccacttttc catcttgcac ttgtaaatcc acagtttcct gagtatcggg ataactggct
     1981 tttatttgca
//
LOCUS       DAOQJE010000739         1990 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_46489_length_1990_cov_5.132300, whole genome shotgun sequence.
ACCESSION   DAOQJE010000739 DAOQJE010000000
VERSION     DAOQJE010000739.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1990)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1990)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1990
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46489_length_1990_cov_5.132300"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..725
                     /locus_tag="PLT08_15940"
     CDS             <1..725
                     /locus_tag="PLT08_15940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE06018.1"
                     /translation="LAEEVLSYIDWNGPWWLYMDWLLLGIFIFMSITIVARANIKTDV
                     LIVFVGVCGGLAIESWGTQTNLWHYYTAERPPLWIIPAWPIASLSIDRITRFMNWSIQ
                     KLEARTQQPVHRSIFPILYWMTFASFLTLMLVFVSPTFDKSYTVLATLLCVMLILTPT
                     DYRYALLTFIAGIGLGYFLELWGTTRQCWTYYTFETPPFFAVLAHGMAAVAFWRAGLL
                     VKMVTEKLVGGKWKIDRAMENE"
     gene            966..1583
                     /locus_tag="PLT08_15945"
     CDS             966..1583
                     /locus_tag="PLT08_15945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HNE06019.1"
                     /translation="MSVRNAILGLLAQKSRHGYELHSAFSTVMGGATWDVKPAQIYTT
                     LERLQESGLVQTKSDLGEGREPDRRIYAITRDGHEALKSWFNESVPTEHQRDEFYVKL
                     MIGLISGEADPARIIQTQRSRLFQEMHDATTQRDKYDPRLEMAKILLSDKAIMHLEAD
                     LRWLDMIEMRLETIKDQPMPEPEIRKRGRPKKIDAGSTYPPPDEK"
     gene            1642..>1990
                     /locus_tag="PLT08_15950"
     CDS             1642..>1990
                     /locus_tag="PLT08_15950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNE06020.1"
                     /translation="MSNIQIEKLTKTYGSGITQVTALDHVNLIIREGEFVAIMGPSGC
                     GKSTLLHLLGGLDKPSEGSVLIGGHNLTDLNDDKLTELRRRKIGFVFQFFNLIPVLNA
                     TENAALPVTLDGLK"
BASE COUNT          479 a          473 c          500 g          538 t
ORIGIN      
        1 cgctggcaga agaagtcctc tcgtatattg attggaacgg tccctggtgg ttatatatgg
       61 actggctact actcggcatt tttattttca tgtccatcac cattgtggcg cgtgccaaca
      121 tcaagaccga tgtattgatt gtttttgtag gggtgtgcgg cgggcttgct atcgaatctt
      181 ggggcaccca aactaacctg tggcattact acacggctga acgcccgcct ttgtggatca
      241 tccctgcctg gcccattgcg agtctctcca ttgaccgcat cacccgtttt atgaattggt
      301 cgatccaaaa gttggaagcc cggactcaac aacctgtcca tcgctcgatc tttcccattc
      361 tttattggat gaccttcgcc tctttcttga ccttgatgct ggtctttgtt tccccaacct
      421 ttgacaagtc ctataccgtt ctcgccaccc tcctgtgtgt aatgctcatc ctcacaccga
      481 cagattatcg ctatgccttg ttgaccttta tcgcaggcat tgggctggga tacttcctag
      541 agctgtgggg caccacccgt cagtgttgga cgtattacac ctttgagact ccgcctttct
      601 ttgcggtgtt ggcgcatggt atggcggctg tcgctttttg gcgggcaggg ttgctggtga
      661 agatggtgac cgagaagttg gtgggtggga agtggaagat agacagggct atggagaatg
      721 agtagccagt aacgccaaat gagtggatga cgaaatccgc gagagtttta ctcttgcgga
      781 ttttttattc gcgtaggcta atagacaaac ttccagtggg gaatcgcttt cagaaagccg
      841 tatcaagata cggtcaagaa agaggcggac gtgattttat tcctcttgac aaaaaatagt
      901 aactcgtata atattttcaa gttttgtaat tagtaactcg tatactattt ttaagtgagg
      961 cactcttgtc cgttcgaaat gccattttag gtctacttgc gcaaaaatcc cggcatgggt
     1021 atgaactcca ttctgccttt tccacggtaa tgggtggggc tacttgggat gtgaagcctg
     1081 cccagatcta caccactctt gaacgtttgc aagagtcagg cttggtgcag accaagtcgg
     1141 atctagggga ggggcgggaa ccagatcggc gcatttatgc catcactcgc gatggacatg
     1201 aagcgctgaa atcgtggttc aacgaaagtg tgccgaccga acaccagcgt gacgagtttt
     1261 acgtcaaact gatgatcggg ttgatctctg gtgaagctga cccggcgcgc attatccaaa
     1321 cccagcgttc acggcttttt caggaaatgc atgacgccac gacgcagcgc gataaatatg
     1381 atccgcgctt ggagatggca aagattcttt tgagcgataa agccattatg cacctcgaag
     1441 cggacttacg ctggctcgat atgatcgaaa tgcgtttgga gaccatcaaa gatcagccca
     1501 tgcctgagcc tgaaattcgc aagcgcggca gacctaaaaa gattgatgca ggttctacgt
     1561 atccgccacc agatgagaaa taagtcatag tagttgcaag ttttattcgt tcgcattgct
     1621 tttataaaga gaaagaaaaa aatgtccaat attcagatcg aaaaacttac caaaacttat
     1681 ggctcaggca tcacgcaagt gaccgcgctt gaccatgtta accttattat tcgtgaaggc
     1741 gaatttgtcg ccattatggg acccagcggc tgcggcaaat cgactctgtt gcatttgctc
     1801 ggcggcttgg ataagcccag tgaaggcagt gtgttgatcg gcgggcacaa cctgaccgac
     1861 ctcaacgatg acaaactcac cgaactgcgt cgccgcaaga tcggctttgt cttccaattt
     1921 ttcaacctaa ttccagtttt gaacgctaca gagaacgctg cgttgcctgt cactttggat
     1981 gggctcaagc
//
LOCUS       DAOQJE010000740         1989 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_46531_length_1989_cov_6.550672, whole genome shotgun sequence.
ACCESSION   DAOQJE010000740 DAOQJE010000000
VERSION     DAOQJE010000740.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1989)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1989)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1989
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46531_length_1989_cov_6.550672"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            266..>1989
                     /locus_tag="PLT08_15955"
     CDS             266..>1989
                     /locus_tag="PLT08_15955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE06021.1"
                     /translation="MQRTMWGVVFDGIYFRLFLWASTLFLVSFLITRGTTLVEWKSFL
                     VALILTSSVFTIEISLQGVTDYPFSMGWSEGNRIWDYSMRFGRDRYGIADNQDIYVLL
                     DSGRMLIGGLPFLIPNVTIEMVRLWIGLTLILPYFFVGIAAYIVAEKNIRIWMLMTLW
                     VFLFLRQGPIHSPLVLVAALTIFIWRKPLWLAIPLIIYAGYFAQASRFTWLFAPGLWI
                     GILELAGASLSNEKLDRAHWVRTITLGLSGAFGGFILPKLMLLLHVARTSSTPIDVTE
                     QIANTGVNSEFVTYAVTDQPLLWYRLLPNSTYGTGILLGLVIAVLPIILWLVWLSASK
                     KWELNGWQKLAIVGPLLAFLGVGLVASTKIGGGGDLHNMDMFLIGVAFTVFMAWVNGG
                     KESVMNNEMPSWVRLTLIAALVFPAVTPIRQLRSYNHGDEINTLLVLTDKTPAEAKSF
                     DMLPDSATVDTALTEIQEAVDEYQKIGDVLFIDQRQLLTFDYIHEEKFIPEYEKKVLM
                     NEALSTNYEYYSSFYSDITNQRFSLIITEPLRTPLKDSSFEFGEENNAWVEWVSIPVL
                     CYYQEIQT"
BASE COUNT          460 a          383 c          451 g          695 t
ORIGIN      
        1 atggatactc gttgaggcat gtgtcgagtt ctataatgtg gcgtggggta ctggcaaggc
       61 tgtggggtta ttctcgccac gttggcttat cctttttgtt tcttttgttt tactatgcgt
      121 gtttgtatat gtatgtgtgg gcattgctgt tcttaaccgc gaaagatttt ttgctttaat
      181 tcaaaacata gttgtattaa gaaatagaat accaacatta agctggattc ttgcaatttt
      241 atttttgact tttccagcgt ggtttttgca acgcaccatg tggggtgttg tttttgatgg
      301 catttatttt cgtttgtttt tatgggcatc aacgcttttt cttgtttctt ttctgattac
      361 gcgtggaacc acattggttg aatggaaatc cttccttgtt gcattgatac tcacttcaag
      421 tgtttttaca attgaaattt ctttgcaagg tgtaacggat tatccattct ctatgggctg
      481 gtctgaaggc aatcgcatct gggattattc aatgcgtttc gggcgtgatc gttatggcat
      541 tgctgacaat caggatattt atgttttgct tgattctggt cgtatgctaa taggcggttt
      601 gccatttttg attcccaatg tgactattga aatggtgcgt ttatggattg ggcttacttt
      661 gatcttgccc tatttttttg ttgggattgc tgcttatatt gttgccgaaa aaaatattcg
      721 tatttggatg ttgatgactt tatgggtttt tctgtttctg cggcagggtc ccatccattc
      781 tccgttagtt cttgttgcgg cattaactat cttcatctgg cgtaaaccat tgtggcttgc
      841 aattccattg attatttatg ctggatattt tgcccaagcg agtcgattta cctggttgtt
      901 cgctcccggt ctatggatcg gaatacttga attagcaggt gcttctttat ctaacgagaa
      961 attggatcga gcccattggg ttcggactat cactttaggc ttgtccggcg catttggcgg
     1021 attcattttg ccaaaattga tgttacttct gcatgtggca cgaacttctt caacaccaat
     1081 cgatgttaca gaacaaattg ccaatactgg cgtcaactca gaattcgtga cctatgcagt
     1141 gaccgaccaa cccctcttgt ggtatcgcct actgcccaac tcaacctatg gaactggtat
     1201 tttgcttggg ctggtcattg ccgtattgcc gattattctt tggttggtct ggctttctgc
     1261 ctctaaaaaa tgggaattaa atggttggca aaaactagcc attgttggac ctctccttgc
     1321 cttccttggg gtaggcttgg ttgccagtac gaagatcggc ggcggcggcg acttgcacaa
     1381 catggatatg ttcttgattg gtgttgcttt tacggttttt atggcttggg ttaatggcgg
     1441 taaagaatct gtaatgaaca atgaaatgcc gagctgggta cggctcacac tcattgccgc
     1501 cttggttttc cccgcagtaa cgccaattcg ccagttgcgt tcatataatc acggcgacga
     1561 gattaatacc ttgttggtcc tgactgacaa aaccccagca gaagctaaat cttttgatat
     1621 gctgccagat agtgcaacag tcgacactgc tttaaccgaa attcaagaag cagtggatga
     1681 gtatcaaaaa attggagatg ttttattcat agatcagcgc caattgttga cgtttgacta
     1741 tattcatgaa gagaaattca ttcctgaata tgaaaagaaa gttctcatga atgaggcgtt
     1801 aagtacgaac tacgaatact attcatcttt ctatagcgat atcaccaatc aacgtttttc
     1861 gctgatcatt actgaaccct tgcgtacccc gctcaaagac agttcttttg aatttggcga
     1921 agagaacaat gcttgggttg aatgggtatc cattcctgtg ctctgttatt atcaagagat
     1981 acagacgta
//
LOCUS       DAOQJE010000741         1989 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_46532_length_1989_cov_6.257497, whole genome shotgun sequence.
ACCESSION   DAOQJE010000741 DAOQJE010000000
VERSION     DAOQJE010000741.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1989)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1989)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1989
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46532_length_1989_cov_6.257497"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..722)
                     /locus_tag="PLT08_15960"
     CDS             complement(<1..722)
                     /locus_tag="PLT08_15960"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HNE06022.1"
                     /translation="MNFSKFNLAVVIPAYRTEKEILAVLQGIPTFIRHIIVVDDASPD
                     SSADLVTTAAKKNRHILLIRHTKNQGVGGAMVTGFKKALELGAEIVIKLDGDGQMDPH
                     YIPALITPLITGEADYAKGNRFRDFDALRQMPIVRRIGNLGLSFLTKAATGYWNIFDP
                     TNGYFAIRAEMLAQLPLDQIDKGYYFETSMLSRLYLRDAFVQDVTIPARYRNEVSSLS
                     IKRVLFEFPYKLTRTLIKRIIL"
     gene            775..1923
                     /locus_tag="PLT08_15965"
     CDS             775..1923
                     /locus_tag="PLT08_15965"
                     /inference="COORDINATES: protein motif:HMM:NF013736.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNE06023.1"
                     /translation="MATGERILIVESDPDIADLIGRQALKPLGYQVTVVGDAGSAIKF
                     AAQTPPDLILANINLPGLSGKDLLAALNSQNIKSPVIVIAEKGQEPDAIQAFRLGAMD
                     VMFWPLRDAEAVSIVERAMRQTQEARERQKLDRQLKATNDELQRRLRDMTTILTIAKA
                     VTSVNDQRYLFDRILESALQLGEAEMCWLMLREDKGNLFLLRAQRGLPEAWAKKMNQP
                     MDDGISSLVALSGESLFMNGSALQKFKIASLGKSAGVVPIKIKNEVIGLMIVVRKGEK
                     EFVKDSQTMLEAMADYASISLVNARLFRALEQTAENARTGEKARYASLETLRKSIRTE
                     VESAVYPLNLVLTEMPGALNPEQKKALETVKTALQKLSHASENTVTSK"
BASE COUNT          539 a          466 c          499 g          485 t
ORIGIN      
        1 ttgaggatga tgcgtttgat gagagtgcgg gtgagcttat atggaaattc aaacaataca
       61 cgtttgatcg aaaggctcga aacttcattg cgataacgcg ccgggatggt cacatcttgc
      121 acgaaagcat cgcgcagata taggcgcgag agcatggatg tttcaaagta atagccttta
      181 tcgatttggt cgagcggaag ttgcgccagc atttcagcac ggatcgcaaa atagccattg
      241 gtggggtcaa agatattcca atacccggtg gcagctttgg tgagaaaact taagccaagg
      301 ttgccaatgc gccgtacgat tggcatttgc cttaacgcat cgaagtctcg aaagcgattg
      361 cctttggcat aatccgcttc gccggtaata agcggtgtaa ttagtgccgg aatatagtgc
      421 gggtccattt gtccatcgcc atcaagtttt attacaattt ccgcaccaag ttccaaggct
      481 ttcttaaaac ctgttaccat ggcgccacct accccttgat tttttgtgtg acggatgagg
      541 aggatgtgac gattcttttt ggcggcagta gtgaccaggt ccgcgctgga atcgggcgaa
      601 gcatcatcca ccacaataat gtggcgaatg aaagtaggaa ttccctgcag aaccgcgaga
      661 atttcttttt cggttcgata ggcgggtatc acaacagcaa ggttgaattt cgaaaaattc
      721 atcttcaaga ttgtaatcga aaaggtgata gaatcttttg aaggagattt acacgtggct
      781 accggggaac gcatcctcat tgttgaatca gacccagata ttgccgattt gatcgggcga
      841 caggcgctta aaccactcgg atatcaagtg accgtggtgg gcgatgcagg ctctgcgatc
      901 aaattcgcgg ctcagactcc gccagacctg atcctcgcca atatcaattt acccggcttg
      961 agcggaaaag acctactcgc cgcgttgaac tctcagaaca taaaatcccc tgtcattgtt
     1021 atcgctgaaa aaggacaaga accagacgcc attcaagctt ttcgccttgg cgccatggac
     1081 gtgatgttct ggcccctgcg cgatgcagaa gccgtctcca ttgtagagcg tgccatgcgt
     1141 cagacgcagg aggcacgtga acgccaaaaa cttgaccgcc aattaaaagc gactaatgac
     1201 gaattacaac gtcgcctgcg cgatatgacg accattctaa ccattgctaa ggctgtcact
     1261 tcggtcaacg accaacgcta tctgtttgac cgcatccttg agagtgcctt gcaacttggc
     1321 gaagccgaga tgtgttggct gatgctgcgt gaagataaag gcaatctctt cttgctgcgc
     1381 gcccaacgag ggctccctga agcttgggca aagaagatga atcaaccgat ggatgatggc
     1441 attagttcat tggtggcgct ttccggcgaa agtctgttca tgaacggctc cgcgcttcag
     1501 aaatttaaga ttgcatcgtt aggcaagtct gctggcgttg tccctatcaa gatcaagaat
     1561 gaagttattg ggttgatgat cgttgtgcgc aagggtgaaa aggaatttgt gaaagactca
     1621 caaaccatgc ttgaagccat ggcagactac gcatcgatct cattggtgaa tgcccgcttg
     1681 ttccgtgctc ttgaacaaac cgccgaaaat gctcgcactg gtgaaaaagc ccgttatgct
     1741 tctttggaga cactgcgcaa atccattcgt acagaagtag aatctgcggt ctatccgctc
     1801 aacctggtgt tgacagaaat gcccggcgcg ctaaacccag aacaaaagaa agcattagaa
     1861 accgtgaaga ccgctctgca aaaactttct catgcttctg agaatacagt cacatccaag
     1921 taaggatata caaagaaagt aatgtcaaaa caagacctga ttttgctggc attggatgca
     1981 tcgcccacg
//
LOCUS       DAOQJE010000742         1989 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_46566_length_1989_cov_2.567735, whole genome shotgun sequence.
ACCESSION   DAOQJE010000742 DAOQJE010000000
VERSION     DAOQJE010000742.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1989)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1989)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1989
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46566_length_1989_cov_2.567735"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..818)
                     /locus_tag="PLT08_15970"
     CDS             complement(<1..818)
                     /locus_tag="PLT08_15970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE06024.1"
                     /translation="MSNHSQTIVPISTWCYVSPELGYKGYVIDVGLMAEALIYYDTVY
                     VNITNQPQFATFVEWFIKQGKYDEFLSLVSDGVIQFYDYSFFTSAINKDGVMSIWNIQ
                     DPIQEKPDTFEQRFLYHEAVQAVLPTGRKRKYFYSTLRNRVIEVKSNEFGTAIENARK
                     DNEQPRRNALILQSFVDELYKFKNLGHPPQIEAKVTKSNEHTNIQWNINFAEITRLAG
                     KELHFHEQTPLNASAISNRLIWSAASLNCDLYLSKPMSIVTGDKLYETGYRVLKT"
     gene            922..1989
                     /locus_tag="PLT08_15975"
                     /pseudo
     CDS             922..1989
                     /locus_tag="PLT08_15975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611236.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT          547 a          445 c          478 g          519 t
ORIGIN      
        1 gttttcagaa cgcggtaacc agtttcatac aatttatcac cagttacaat actcatgggt
       61 ttgcttaagt acaaatcgca attcaaactc gccgctgacc aaataagccg attggaaatg
      121 gcacttgcat tgagcggcgt ttgttcgtga aagtgcaact ccttgcctgc taaccttgta
      181 atttcggcga agttaatatt ccactggata ttagtatgct cattcgattt tgttactttt
      241 gcttctatct gtggaggatg acctaagttt ttgaatttat acaactcatc tacaaaagat
      301 tgaagaatta acgcatttct gcgaggttgt tcattatctt ttcttgcatt ttcaattgct
      361 gttccaaatt catttgattt aacttcaata acccgatttc ggagagtgct gtaaaaatac
      421 tttctcttcc ttcctgtggg aagaactgct tgaactgctt catgatacaa aaatcgctgt
      481 tcaaatgtgt ctggtttttc ctgtataggg tcttggatat tccatattga cattacccca
      541 tctttattga ttgctgatgt aaaaaaactg taatcgtaga attgaattac gccatcagat
      601 actagagata aaaattcgtc gtatttccct tgttttataa accattcaac aaaggttgcg
      661 aattggggct gattagtgat attgacatag acagtatcgt aataaattaa tgcttccgcc
      721 attaagccaa cgtcaattac ataaccttta tatcccaatt cgggagagac atagcaccaa
      781 gtgctaattg gtactatagt ttgagaatga ttcgacataa gttttttttt tatttactat
      841 aaaccaacct gcccctctgt gtctataatc gtgagacaat tgttgccaat aatttagaga
      901 gcagggcgca ggagaaacgc gatgaaagag gaagagaacg gcaagacgga agaaagcctg
      961 acgacagaag tagttgtcaa accgaaacgt cggcagcata gtgcggaata caaactacgg
     1021 atcctccagg aactggatga atgtagtggc aaaggggaga taggggcggt cttgcgtcga
     1081 gaagggctgt attcgtcgtt ggtcagtaaa tggcgggaac agcgtgagaa aggtagcctg
     1141 aacggattat ccggacaaaa gcgtggtccg aaagttgatc cgaatgcagc ggaactggcg
     1201 cgtctacaac gcgagaacaa acgcctgaag gagcaattgg agcgtgcgga actgatcatt
     1261 gaagtccaaa aaaaagtcgc ccgactgatg ggcgagaccc ctctcccatc caacgaggag
     1321 aactgatgca gaccgcagag gcgttgtcca agcgggtagg caaacgaaaa gcctgtgctg
     1381 tgttgggaat cccgcgcagc cgtctatacc ggaaagaaaa tctgcgatcg gtagccagac
     1441 cctctccccg tgccctcagc caccctgagc gagaagccgt gcgtcaggaa cttaacagcg
     1501 agcgttttca ggactgcgcc ccgcgggaag tgtatgccag tttgctggac gagggtcgct
     1561 acctgtgttc ctggcgtagc atgtaccgca ttctggatga gcatcaggag gtacgtgaac
     1621 gccgtaacca gttgcgacat ccaagctata tcaagccaga actactggcg acagccccaa
     1681 atcagctctg gagttgggat atcaccaaac tcttgggacc gtctaaatgg acgtattact
     1741 acttgtacgt gatcctggat gtctacagcc gttacgtggt gggttggatg atcgccgaac
     1801 gggaatccgc cgcattggcg gaagccttga tctcgcaaac ctgtctgcgg caaggcatcc
     1861 aacgtggaca actcaccatc catgctgatc gcggcagtgc tatgacttcc aaaccggtag
     1921 cgttgttgct ggcggacctg ggtgtcacca aaactcattc ccgtccgcat gtttccaatg
     1981 acaatcctt
//
LOCUS       DAOQJE010000743         1987 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_46638_length_1987_cov_3.167702, whole genome shotgun sequence.
ACCESSION   DAOQJE010000743 DAOQJE010000000
VERSION     DAOQJE010000743.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1987)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1987)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1987
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46638_length_1987_cov_3.167702"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..326
                     /locus_tag="PLT08_15980"
     CDS             <1..326
                     /locus_tag="PLT08_15980"
                     /inference="COORDINATES: protein motif:HMM:NF015113.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="His/Gly/Thr/Pro-type tRNA ligase C-terminal
                     domain-containing protein"
                     /protein_id="HNE06025.1"
                     /translation="FPVWLSPEQVRVISITDEQNDYAEKIAKELRENGVRASADLSSQ
                     RMNAKIRNAQMMKVPYMIVVGKNEMEANQISLRVRDGSQQNGFALSEFIARAKDRISR
                     RASEL"
     gene            429..959
                     /locus_tag="PLT08_15985"
     CDS             429..959
                     /locus_tag="PLT08_15985"
                     /inference="COORDINATES: protein motif:HMM:NF013460.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b/b6 domain-containing protein"
                     /protein_id="HNE06026.1"
                     /translation="MSTDLQPKRYHPVHVTLHWLTALLVFAPLLSGFLILARTPDAQE
                     AQFLGMHKWIGIFLGLVIIARIITRFVFKRPAPADAGHPLLNWVGAIVHFLLYAGIFA
                     MLFSGDALDEAYGLEGILAGNGTMPESVMVYAEWARHSLFSYTLAALVALHVGAALYH
                     QFIRKDNLLSRMWYGK"
     gene            complement(1015..1812)
                     /locus_tag="PLT08_15990"
     CDS             complement(1015..1812)
                     /locus_tag="PLT08_15990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE06027.1"
                     /translation="MELNFATITLPTIVVWMLGLFIFLLGSLLGYFNMSIDARRKMEG
                     VDQKIENARMEADRRIAEMQKRLDEANIQPATIQQSTTAPSLLTLKNINNLQTQIEMD
                     GQLLIAPLSPERKKRLVELINHFRPWIEGTVPATSQPISKPLTAPLSESPTDHKKNEA
                     VPYVPPTVKPVPVVLGLNNQKTKTDPESEFKLLSMVKQIDVVLQKRLSGTPLESLGIH
                     LNDSLHGGLEVQIGSQKFETIDDVPDKNIQTAIRAAIAEWEQKYVPR"
BASE COUNT          504 a          430 c          465 g          588 t
ORIGIN      
        1 acttcccagt ttggctctcg ccggaacagg tgcgcgtcat ctcgattacg gatgaacaga
       61 atgattatgc tgaaaagatc gccaaggaat tgcgcgagaa cggtgtccgt gccagtgcag
      121 atttgtcctc tcagcgcatg aatgccaaga tccgcaatgc acagatgatg aaggttccgt
      181 atatgatcgt ggtcggaaag aacgagatgg aagccaacca gatctcgttg cgagtccgcg
      241 atggcagtca gcaaaatggg tttgccttga gcgaatttat tgcccgggcg aaagatcgca
      301 tctcccgccg cgccagcgaa ctgtaatttt caacaccgga gaaatacttc tccggtgttg
      361 tcttttaatt gaaactctga aaaagattcg gggttttatc tgcataccaa accaaggaga
      421 gaaagattat gtctactgat ttacagccca aacgttatca tcctgtccat gttaccttgc
      481 attggctgac cgcactcttg gtctttgcgc ctttactttc aggctttttg atccttgccc
      541 gcacccctga tgctcaagag gcacaattcc tcggtatgca taaatggatt ggtatttttc
      601 tcggacttgt cattattgca cgtatcatca ctcgttttgt gttcaagcga cctgctccag
      661 cagatgccgg gcatccactt ttgaactggg taggcgctat tgtccacttc ttgttatatg
      721 cagggatttt tgccatgctc ttctcaggcg atgcacttga tgaagcctac ggcttggaag
      781 gtattctggc tggcaacgga acgatgcctg aaagtgtgat ggtctatgcc gaatgggcaa
      841 gacatagtct gttttcgtat accctggcgg ctttggtcgc tttgcatgta ggcgcagcgc
      901 tctatcatca attcattcgt aaagataatt tgttgtcacg tatgtggtat gggaagtaaa
      961 aaaagaatat aacaaaaaag ccctgctcac aagagcaggg cttttttgtt attattagcg
     1021 aggtacatac ttttgttccc actctgcaat tgcagcacgg atagcagttt gaatattctt
     1081 atcaggaaca tcatcaattg tctcaaattt ttgtgagcca atttgtactt ccagtccacc
     1141 atgtagtgag tcgttgagat gaattcccag agattctaga ggcgtccctg atagtctttt
     1201 ttgtaataca acatcaatct gcttgaccat gcttagtaat ttgaactcgg actcaggatc
     1261 tgttttggtc ttttgattat tcagcccaag aacgacagga actggcttta ctgtaggtgg
     1321 cacataagga acagcctcat tttttttatg atcagttgga gattcagaaa gaggcgcagt
     1381 gagaggtttg gaaataggct gagatgttgc agggacggtc ccttcaatcc acgggcgaaa
     1441 atgattgatt agttctacca aacgtttttt tcgttcagga gaaagaggag cgattaagag
     1501 ttgcccatcc atttcaattt gcgtttgtag attatttatg tttttcaagg tcagcaaaga
     1561 aggggcagta gtagattgct ggattgtagc aggttgtata ttagcttcgt ctaatctttt
     1621 ttgcatttca gcaattctac gatctgcttc catacgtgca ttttcgatct tctgatcgac
     1681 accttccatt tttctgcgcg catcaatgct catattgaaa tagccaagca agctgccaag
     1741 tagaaagata aaaagcccca acatccatac aacaattgtg ggaagagtga tggtggcgaa
     1801 attaagctcc atggttattt cctttcaagc ggttggcatg ttgggcatac atgcgtgcca
     1861 cgttgcccaa cggttagctt ttgaatttcc gtaccgcaaa cgagacaagg tgtcccttcg
     1921 cggtcgtata ctcgaaaatg attttgatac tcaccgccgc gatagaccca atcgatgctg
     1981 gcaccgt
//
LOCUS       DAOQJE010000744         1983 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_46804_length_1983_cov_6.503112, whole genome shotgun sequence.
ACCESSION   DAOQJE010000744 DAOQJE010000000
VERSION     DAOQJE010000744.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1983)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1983)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1983
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46804_length_1983_cov_6.503112"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            176..973
                     /locus_tag="PLT08_15995"
     CDS             176..973
                     /locus_tag="PLT08_15995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE06028.1"
                     /translation="MELNFATITLPTIVVWMLGLFIFLLGSLLGYFNMSIDARKKQEL
                     AEQKVEHARIEADRRIAEMQKRLDEADIQPAKVQQTATTPSLLTLKSVNNLQTQIEMD
                     GQLLIAPLSPERKKRLVELINHFRPWLEGAASTSQPISKPLTTPLSEPLTNFKKPEAV
                     PYVPPTVKPVPVILGVNNQKTKTDPESEFKLLSMVKQIDVVLQKRLAGTSLESLGIHL
                     NDSLQGGLEVQIGSQKFETIDDVPDANIKAAIRAAIAEWEQKYIPGQ"
     gene            complement(1028..1558)
                     /locus_tag="PLT08_16000"
     CDS             complement(1028..1558)
                     /locus_tag="PLT08_16000"
                     /inference="COORDINATES: protein motif:HMM:NF013460.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b/b6 domain-containing protein"
                     /protein_id="HNE06029.1"
                     /translation="MSTDSQPKRYHPVHVTLHWLTALLVFMPLLSGFLILARTPDAQE
                     AQFLGMHKWIGIFLGLVIIARIITRFVFKRPAPADAGHPLLNWVGAIVHFLLYAGIFA
                     MLFSGDALDEAYGLEGILAGNGTMPESVMVYAEWARHSLFSYTLAALVALHVGAALYH
                     QFIRKDNLLSRMWYGK"
     gene            complement(1658..>1983)
                     /locus_tag="PLT08_16005"
     CDS             complement(1658..>1983)
                     /locus_tag="PLT08_16005"
                     /inference="COORDINATES: protein motif:HMM:NF015113.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="His/Gly/Thr/Pro-type tRNA ligase C-terminal
                     domain-containing protein"
                     /protein_id="HNE06030.1"
                     /translation="FPVWLSPEQVRVISITDEQNDYAEKIAKELRENGIRASADMSSQ
                     RMNAKIRNAQMMKVPYMIVVGKNEMEAGQISLRVRDGSQQNGFALSEFIARAKDRISR
                     RASEL"
BASE COUNT          571 a          478 c          440 g          494 t
ORIGIN      
        1 acggtgccag catcgattgg gtctatcgcg gcggtgagta tcaaaatcat tttcgggtat
       61 acgaccgtga agggactccc tgcctcgttt gcggtactga aattcaaaag ctaaccgttg
      121 ggcaacgtgg cacccatgta tgcccaacct gccagccgct tgaaaggaaa taaccatgga
      181 gcttaatttc gccactatca ctcttcccac catcgttgta tggatgctag gactttttat
      241 ctttttactt ggcagcttac ttggctattt caatatgagc attgacgcgc gcaagaaaca
      301 agaactggca gaacaaaagg tagaacatgc gcgtattgaa gcagatcgta gaattgctga
      361 aatgcaaaaa cggttagatg aagctgatat acagcctgcc aaggttcagc aaactgccac
      421 tacaccttct ttgctaacat taaaaagcgt aaataatcta caaactcaaa tcgaaatgga
      481 tgggcaacta ttaatcgccc ctctttctcc tgaacgaaaa aaacgtttag tggaacttat
      541 caatcatttt cgtccgtggc ttgaaggggc tgcatcaaca tcccagccta tttctaaacc
      601 tctcactacg cccctttctg aacctctaac taattttaag aagccggagg ctgttcctta
      661 tgtgccacct actgtaaagc cagttcctgt cattcttgga gtgaataatc aaaaaaccaa
      721 aacagaccct gagtctgagt tcaaattact aagcatggtt aagcagatcg acgttgtatt
      781 acaaaaaagg ttggcaggga cgtctctaga gtctctgggc attcatctca acgactcatt
      841 acaaggtggc ttggaagtgc agattggttc acaaaaattt gagacaattg atgatgtacc
      901 cgatgcaaat atcaaagctg ccatccgtgc agcaattgca gaatgggaac agaagtatat
      961 ccctggacaa taatttcgaa aaaagccctg ctcttgtgag cagggctttt ttactgttat
     1021 atgcttttta cttcccgtac cacatacgag acaacaaatt atccttgcga ataaattgat
     1081 gatataacgc cgcgcccaca tgcaaagcga ccaaagccgc cagagtgtac gaaaacagac
     1141 tatgtcttgc ccattcggca tagaccatca cactttctgg catggtcccg ttgcctgcta
     1201 aaatgccttc caaaccatag gcttcatcaa gcgcatcgcc tgagaacagc atggcaaaaa
     1261 tccctgcata tagcaagaag tggacaatag cacccaccca gttcaaaagc ggatgcccag
     1321 cgtctgctgg agccggacgc ttgaatacga agcgggtaat aatacgggca ataatgacaa
     1381 gaccgagaaa gataccgatc catttatgca tgccgaggaa ttgtgcttct tgagcatccg
     1441 gagtgcgggc aaggatcagg aaacctgaaa gcaaaggcat aaagaccaaa agtgcggtca
     1501 gccaatgcaa ggtaacgtgg acgggatgat agcgtttggg ctgtgaatca gtagacataa
     1561 tctctccttg gttttgattt tcagataaaa ccccaaatcg ttttcagagt ttcaatagaa
     1621 agacaacacc ggagatgtat ttctccggtg ttgaaaatta cagttcactt gcgcggcggg
     1681 agatgcgatc tttcgcccgg gcaataaatt cgcttaaggc aaagccattt tgctggctac
     1741 cgtcgcggac acgtaacgag atctgcccgg cttccatttc attcttgcca accacaatca
     1801 tatatggcac cttcatcatc tgcgcattgc ggatcttggc attcatacgc tgggacgaca
     1861 tgtctgcact ggcacggatg ccgttctcgc gcaattcctt cgcgatcttt tcggcatagt
     1921 cgttctgctc atccgtaatc gagatgacgc gcacctgttc cggcgagagc caaactggga
     1981 agt
//
LOCUS       DAOQJE010000745         1978 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_47026_length_1978_cov_6.038482, whole genome shotgun sequence.
ACCESSION   DAOQJE010000745 DAOQJE010000000
VERSION     DAOQJE010000745.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1978)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1978)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1978
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47026_length_1978_cov_6.038482"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..200)
                     /locus_tag="PLT08_16010"
     CDS             complement(<1..200)
                     /locus_tag="PLT08_16010"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HNE06031.1"
                     /translation="MPNTTFNSRRSPVYSRRGIVATSQPLATAAGIEVLSKGGNAADA
                     AVAAAAALNVTEPTSTGIGGDM"
     gene            317..1732
                     /locus_tag="PLT08_16015"
     CDS             317..1732
                     /locus_tag="PLT08_16015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE06032.1"
                     /translation="MKKLFSLFFAISLILSACGTVSTTPAQTGMPIVTHTATQTVTST
                     ITPLPTFTPTFDVSTIVTVTPAEKAECPTSIPYPEKDFDLFEFWENGNYQSPEEAALA
                     LLNKYGSEVLVNYERSFGDSPETMAFQDFTNDGVPELAIRLTLNTSFSSFDIFGCQDG
                     EYHLLLSIPSNGLGYAPIIVSIEDNNKNSVPELTILEGYLSQGGHSFGVYEWDGEKFT
                     SLLPPNHPDQPDSTFLWVEATGKIYYKDVDSDFLNELILESGIPVWEIYYVGLPWRNN
                     STYYKWNGQVFAPYKSEFTEPEFRFQAVQDADLAFTQQEYGKAVVLYQEAIFSDELRS
                     YSPEIRENLRAIWEAQYGEVPTPTPYPIEDPTEYPSLAAYAYYRIMLLHLVQGQEAEA
                     ESTYQTLQDTFGADPYAASYVEMATKFWEAYQSTNKMYDGCAAAIQYAVEHPQILTPL
                     GSDYHGWQSHIYVPADVCPFR"
BASE COUNT          561 a          455 c          451 g          511 t
ORIGIN      
        1 aacatatctc cgccaatccc cgtggaggtt ggctcggtaa cattgagcgc ggcagcggca
       61 gccacagcgg catcagcggc attgcctccc tttgaaagga cttcaatgcc cgcggcagta
      121 gccaacggct gcgaggtggc gacaattcca cggcgggagt acacgggcga acgacgagag
      181 ttgaatgtgg tgttgggcat gggtttctct atgaattggt tttgctgaaa gctgagcgct
      241 gattgctaga tagcgttcag ctttcagcac tcagccagta ttttctcgaa gtataatccc
      301 atcaatgagg tggacgatga aaaaactttt ctcccttttc tttgcaatca gcctaatcct
      361 ctccgcttgt gggacagtat ccaccactcc tgcacaaaca ggaatgccaa tcgtaacgca
      421 cacggcaact caaacagtta cttccaccat tacacccctg cccaccttca ccccgacttt
      481 cgatgtttcg acgattgtca cggttacgcc tgcggagaag gcggagtgcc caacatccat
      541 cccatatccc gaaaaagatt ttgatctttt tgaattctgg gagaacggaa attatcaaag
      601 tcctgaagaa gctgccttag cattgcttaa caaatatgga tcagaagtac tggtgaacta
      661 cgaaagaagt tttggggata gtccggaaac catggctttt caagatttca ctaatgacgg
      721 agtacctgag ttagctatta gattgaccct taatacttca tttagctcat ttgacatatt
      781 cggttgtcaa gacggtgaat accatctttt attatccatt ccttcgaatg gattaggtta
      841 tgctccaatt attgtgtcga ttgaggataa caataaaaac agcgtgcccg agttgacgat
      901 actagaaggt tacttatctc aaggaggaca ttcatttggg gtctacgaat gggacggtga
      961 aaaatttaca agccttcttc ctccaaatca tccagatcaa ccagattcaa cttttttatg
     1021 ggtagaggca actggaaaaa tctattacaa agatgttgat tctgattttc taaatgagtt
     1081 aattttggaa agtggcattc cggtctggga aatttattac gttggtctgc catggcgtaa
     1141 taacagtaca tattacaagt ggaatggtca agtctttgcc ccatacaaga gcgaattcac
     1201 agaacctgag tttagatttc aagctgtaca agatgctgac ctcgcattta ctcagcaaga
     1261 gtatggcaaa gcagtagtct tataccaaga agcgatattt agtgacgaat taagaagtta
     1321 ttctccagag atacgtgaaa accttcgagc aatctgggaa gcacagtatg gagaagtccc
     1381 taccccaacg ccatatccga ttgaagaccc aaccgaatac ccaagcctcg ccgcttacgc
     1441 ctattaccgc ataatgctcc ttcacctcgt gcaaggacaa gaagcagaag ccgagtccac
     1501 gtaccaaact ctgcaagata ccttcggcgc agacccatac gccgcttcct acgtggaaat
     1561 ggcgactaaa ttttgggaag catatcagtc cacgaataag atgtacgatg gttgtgcggc
     1621 ggcgatccaa tacgcggtgg agcacccgca gattctgact ccgctcggca gtgattatca
     1681 cggctggcaa agtcacattt atgtgcctgc ggatgtctgt ccattcaggt aataacttag
     1741 gtgacagtca gattaaagct gactgtcacc ttttgtaact tgatataatc acgctaattc
     1801 aatgcggagg gcacggaatg actcaagtct atgactacgt gattattgga agtggcttcg
     1861 ggggaagtgt atccgccatg cgattgaccg aaaaaggcta ctcggttctc gtccttgaaa
     1921 aaggcaaacg cttcgaagat aaagactttg caaaatccaa ctggcaatat tggaaata
//
LOCUS       DAOQJE010000746         1976 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_47141_length_1976_cov_2.977095, whole genome shotgun sequence.
ACCESSION   DAOQJE010000746 DAOQJE010000000
VERSION     DAOQJE010000746.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1976)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1976)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1976
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47141_length_1976_cov_2.977095"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(97..792)
                     /locus_tag="PLT08_16020"
     CDS             complement(97..792)
                     /locus_tag="PLT08_16020"
                     /inference="COORDINATES: protein motif:HMM:NF019847.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HNE06033.1"
                     /translation="MLTTKSLKDNFRFFLFWLYIQIHRYLHRIEVLPYSRHFGAERGS
                     PVGRYYVEKFLRSHADDIAGHCLEFGQDRYRTYFPKAIEYDITDVVERPGVRYVCDIH
                     DPVGMPRDHFDAIICTQVFEHLASPEKAARSLYKLMAPGGVLLLTAPFINQVHYDPTD
                     FRRFTPDGLEMIIKEAGFDIEIVTFGGNALVGTGSLLGMVQEDFTTHELEIMDPVYPY
                     NILVKAKKPLLNG"
     gene            complement(922..1587)
                     /locus_tag="PLT08_16025"
     CDS             complement(922..1587)
                     /locus_tag="PLT08_16025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE06034.1"
                     /translation="MSRLIALGDSHLEALKYAAALNLLLSEKNEFCIVPGATAVGLRN
                     PNSATQALEIFKTVLEKGDRASHIILHIGEVDCGFVIWWRAQKYGEPITVQFNESLDS
                     YKRFIDDVLLMGYRKICVTGASLPTIRDGVDFGDVANKRSEVHVSLYERTQLTLKYNQ
                     VLRDFSTQRSLDYFDLSDAFLDRKEKVLNDLFRNPILTDHHLDVQKTCGVWAYVCNRF
                     LAS"
     gene            complement(1589..>1976)
                     /locus_tag="PLT08_16030"
     CDS             complement(1589..>1976)
                     /locus_tag="PLT08_16030"
                     /inference="COORDINATES: protein motif:HMM:NF025877.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Wzt carbohydrate-binding domain-containing
                     protein"
                     /protein_id="HNE06035.1"
                     /translation="EQFVNVLHSKGVYRIEMSGEFTSASSDIYYAIHIRSVSGNVISG
                     QHFPREGDSIESIEAGQKFRVTFEFNMALLPGVYFVGGGIWSRNEPSCLHRILDALIF
                     RVQDVETRYSFGVCDLMSEPGKMELY"
BASE COUNT          654 a          412 c          346 g          564 t
ORIGIN      
        1 aggtattccc aagtggcagt gtttctgaaa ggcaaatatc tttttatcat ttatatagac
       61 tttatagaaa ttatatcaaa tccaattttt aaagtattat ccattgagta aaggtttttt
      121 cgctttaacc agaatgttat acggataaac aggatccatt atctcaagtt catgagttgt
      181 aaaatcctct tgaaccatgc ctagtagtga tccagttcca acaagagcat ttcctccaaa
      241 tgtaacaatt tcaatatcga atccagcctc tttgattatc atttcaagac catcaggtgt
      301 aaagcgtcga aaatctgttg gatcataatg gacttgatta ataaatgggg cagttaataa
      361 gagaaccccg cccggtgcca ttaacttata gagagagcga gcggcttttt cagggcttgc
      421 gagatgctca aatacctgag tacaaataat cgcgtcgaaa tgatctctgg gcattccgac
      481 cgggtcatga atatcacaaa catatctgac accagggcgc tcaaccacat cggtaatatc
      541 atattcaata gcctttggaa agtatgtacg ataacgatcc tgaccaaatt cgaggcaatg
      601 tcccgctata tcatcagcat gagaccttaa gaatttttca acataatacc gtccaacggg
      661 agaacctcgt tctgcaccaa agtggcggct atagggaaga acttcaattc gatgtaaata
      721 acggtgaatt tgtatgtata accaaaagag aaaaaaacgg aaattatctt ttaatgattt
      781 tgttgttagc attttcacct tagagtggag tgagtcaaga aggagtgatt gaaattatta
      841 tctttaatac taattttata actcacaaaa ctgttagcta gatttctgtg ctgttaggta
      901 gtgagtgttt gcactaaaaa gttatgaagc tagaaaacga ttgcacacat aagcccagac
      961 accacaggtc ttttgaacat ccagatgatg atctgtcaaa atcgggttgc gaaacaaatc
     1021 atttaatact ttttcttttc tatccaaaaa agcgtcggaa agatcaaaat aatccaatga
     1081 acgttgtgtt gaaaagtctc ttaatacctg gttatatttc aaggtcaatt gagtacgctc
     1141 gtaaagcgaa acatgaactt ccgatctttt atttgctaca tcgccaaaat caacaccatc
     1201 acgaatagtt ggcaaagaag ctccagttac acaaatcttt cgatacccca ttaagagtac
     1261 atcatcaata aaccttttat aagaatccaa cgattcgttg aattgtaccg taataggctc
     1321 gccatatttc tgtgcccgcc accagatcac aaaaccacaa tctacttctc caatatgcaa
     1381 gataatatgt gaagcacgat cacctttttc gagtactgtt ttgaaaatct caagcgcttg
     1441 agtagcagaa ttcggatttc gcaatccaac agcagtagca cctggaacaa tacaaaattc
     1501 gtttttttca gataatagaa ggtttaaagc tgcagcatac tttaatgcct ctagatggga
     1561 atctcccaat gctatcaagc gagacatcct aatacaattc cattttaccg ggctcagaca
     1621 ttaaatcaca taccccaaat gaatagcggg tttccacatc ctgcactctg aatataagag
     1681 catccaatat tcggtgaaga cagctaggtt cattacgcga ccagataccg ccgccaacaa
     1741 aatagacccc gggcagcaac gccatattga attcaaacgt tactctaaat ttttgtccag
     1801 cctcaatact ctctatgcta tcgccttcac gagggaaatg ttgcccagat attacatttc
     1861 ctgaaactga gcgaatatgt atagcgtaat atatatcact gcttgcagaa gtaaattccc
     1921 cagacatttc aatacgatag acacctttgg aatgaagaac attcacaaac tgttca
//
LOCUS       DAOQJE010000747         1975 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_47171_length_1975_cov_6.090625, whole genome shotgun sequence.
ACCESSION   DAOQJE010000747 DAOQJE010000000
VERSION     DAOQJE010000747.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1975)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1975)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1975
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47171_length_1975_cov_6.090625"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..694
                     /locus_tag="PLT08_16035"
     CDS             <1..694
                     /locus_tag="PLT08_16035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE06036.1"
                     /translation="VHRVDALVKTLQNNYSAAQTSYESAIELEPQSAPLRKWYGSFLM
                     RYMDDTEGALQQFLEAANLDPTACDTQIEMSRAFLYKKDFDEAFDILQNLVKRLDLPA
                     WKRRKIYDLYLQYFYRKADQLLAQRDPVGALSLLQHLKIAYQECAPELIDNTMKRKLR
                     DAIQLSRSCAYFASDEINKNKATELAEWLTSVSVQGLRFSDYKGNAVQLNGKIIMLTE
                     VLQICAAEASGV"
     gene            730..1944
                     /locus_tag="PLT08_16040"
     CDS             730..1944
                     /locus_tag="PLT08_16040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015244411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS701 family transposase"
                     /protein_id="HNE06037.1"
                     /translation="MPAIVALPQVVEELLLQFGDLFPNEPSRVHFAEYLTGLIVAEHK
                     TVSGIAREFADAPDQSCLNRWLTEAKWDVARLNAHRLDWLQNDPATRYRHDGVIPLDN
                     TLIDHEGKLIEDVGYFWDHADKRHLIAHDYLFVNYVNPSGKHYPLDFRRFRKREQCDN
                     EHPFKNHTDLAKELIDWVVVEGIPGEFTFDSYFTNAPIMNHIHRHERTYVGDLKANRV
                     IVVSGQELVLSAWVKTLTPPLRTKFTVGGHTQWYFTKSVRLPNVTHPVRLLILWQSAD
                     AKEPRKILVTNRTHWEAHRILKVYKRRWSGTETFHRDGKQHLGMGECQLRDGMGQTRH
                     MQLVVLVYSALMRQLKHDRALEWAQTRLMTIGESCRCIARETLAKTLAWAVKQAQNGM
                     TLPDIQHRLALA"
BASE COUNT          516 a          490 c          516 g          453 t
ORIGIN      
        1 agtgcacaga gtagacgctc tcgtaaagac tctacaaaat aactattcag cagcacaaac
       61 atcttatgag tctgccatag agctagaacc acaatctgca cctttgagaa aatggtatgg
      121 aagtttctta atgcgttaca tggatgatac tgaaggtgcg ctgcagcagt ttcttgaggc
      181 agcgaatctt gatccaacgg cttgtgacac tcaaatagaa atgtctcggg cgtttcttta
      241 caagaaagat tttgatgaag cattcgacat attacaaaat ctcgtaaaac gtttagatct
      301 tcctgcttgg aagagaagaa agatttacga tctttattta caatattttt acagaaaggc
      361 tgatcaatta ttagcacaga gagatcctgt aggtgctctt agtcttctac agcatcttaa
      421 aattgcttat caggaatgtg cacctgagtt gattgacaat actatgaagc gaaaacttcg
      481 tgatgcaata caattgtcga gaagttgtgc ttacttcgca agcgatgaaa taaataagaa
      541 caaagccacc gaactcgctg aatggttaac aagtgtctca gtgcaaggtc tacgatttag
      601 cgactacaaa ggaaatgcag ttcagttaaa tggcaagatt atcatgctaa ctgaggtttt
      661 gcagatttgc gcggcggagg cgagcggggt atgatttgac cgacgaaagc atataggtcg
      721 gagggtcgta tgccagcaat tgttgcgcta ccgcaggtgg ttgaagaatt gctgctgcag
      781 tttggagacc tgtttccaaa tgagccctcc cgcgtccatt tcgcagagta tctgaccggc
      841 ctgattgtgg cagagcacaa aaccgtcagc ggtatcgccc gcgagtttgc cgatgctcct
      901 gaccagtcct gcctgaaccg ctggcttacg gaagccaagt gggatgttgc tcgcctgaac
      961 gcacaccgtc tcgactggct ccagaacgac ccggctaccc gctatcgtca cgatggtgtg
     1021 atcccgctcg acaatacctt gattgatcac gagggcaaac tgatcgagga tgtcggctat
     1081 ttctgggacc acgccgacaa acgccatctg atcgcccacg attacctgtt cgtcaactac
     1141 gtcaatccaa gtggcaagca ctatccgctc gactttcggc gcttccgcaa gcgcgagcag
     1201 tgcgacaacg agcacccatt caagaatcac accgacttgg ccaaagagtt gattgattgg
     1261 gtggttgtcg agggtatccc aggtgagttc accttcgaca gctactttac caatgcgccg
     1321 attatgaacc acatccaccg ccacgagcgc acgtatgtcg gagacctgaa agccaatcgc
     1381 gtgatcgtgg tcagcggtca ggagctggtg ctgagcgcgt gggtcaagac gctcacgcca
     1441 ccgttacgaa ccaagttcac ggtagggggc cacacccagt ggtatttcac gaagtcagta
     1501 cgactgccaa atgtcaccca cccggtgcgc ctactgatct tgtggcagag tgcagatgcc
     1561 aaagaaccgc gtaaaatcct ggtgacgaac cggacgcatt gggaagccca ccgcattctg
     1621 aaggtgtaca agcgacgctg gagtgggacc gagacctttc atcgggacgg gaagcaacat
     1681 ctgggtatgg gcgaatgtca actcagggac gggatgggtc agacaagaca catgcagctc
     1741 gtcgtcctgg tgtacagcgc tttgatgcgc cagttgaagc acgaccgcgc cctggaatgg
     1801 gctcagacac gcctgatgac catcggcgag tcctgtcgct gcatcgctcg cgagacgctg
     1861 gccaaaacgc ttgcctgggc ggtcaaacaa gcgcagaacg ggatgactct gccggatatt
     1921 cagcatcgcc ttgcgcttgc ctagctttgg gctgatttat gcaaaagctc agtta
//
LOCUS       DAOQJE010000748         1970 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_47450_length_1970_cov_2.502350, whole genome shotgun sequence.
ACCESSION   DAOQJE010000748 DAOQJE010000000
VERSION     DAOQJE010000748.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1970)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1970)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1970
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47450_length_1970_cov_2.502350"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..193
                     /locus_tag="PLT08_16045"
     CDS             <1..193
                     /locus_tag="PLT08_16045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE06038.1"
                     /translation="LDMLDILYEIFPNAPRHVHAEPRAGDIYRSIGTPKKIMNMLGYK
                     PQVTLAEGLKEAVESMRDS"
     gene            259..>1970
                     /locus_tag="PLT08_16050"
     CDS             259..>1970
                     /locus_tag="PLT08_16050"
                     /inference="COORDINATES: protein motif:HMM:NF014740.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate sugar
                     epimerase/dehydratase"
                     /protein_id="HNE06039.1"
                     /translation="MSSRPHVRNRFVLIGDLALTIVSVLGSFALRLDVSELPFYFPAA
                     LVMCAVTLLVKIPTYYFFGLYRRLWVYASTNELRLITFAVTTASVLASGVMFLLIGFD
                     LIKPGMPRSALGIDWLISLILIGGSRFVLRILAEQSSAQANANTRHALILGAGDAGAL
                     VVRELQKTSQLNLIPVGFLDDDPAKQNHNIYGVTVIGKIDQLANTLDSKQVNEVIIAI
                     PSAPGHIIRLVNDECRKRGITSRIIPGFYELIGGKVSVNRLREVDITDLLRRDPAKID
                     DRLIGATLGGKRILVTGAGGSIGSELCRQLARWNPTELVLLGHGENSIFEALQELKND
                     FPALNLQAVIADVRDLNRIEKVFNQHKPQVVFHAAAHKHVPLMEANVEEAVTNNVLGT
                     KNVVETAVRHGVERLVLISTDKAVRPVSVMGATKRLAELAVLDAAQQHNLPYSVVRFG
                     NVLGSRGSVVRTFKNQIARGGPVTITHPEMYRYFMIIPEAVHLVLQAACMGTGGEVFM
                     LNMGEQVRILDLAEDLIRLSGLEPYRDIDIQTTGIRPGEKLREDLLEDGVDFERTQHS
                     EIFR"
BASE COUNT          428 a          575 c          476 g          491 t
ORIGIN      
        1 gctcgacatg ctcgatattc tctacgaaat cttccccaac gcccccagac acgtccatgc
       61 cgaaccgcgc gccggtgaca tctatcgttc catcggtacg cccaaaaaga tcatgaatat
      121 gctcggctac aaaccgcaag tgactttggc ggaaggtctt aaagaggcgg tggagtcgat
      181 gcgtgactcg taatgcgtaa acctaattca taatatacgt atcacgcatc acgttttacg
      241 ccttcactca cattcctcat gtcttctcgt cctcacgttc gcaatcgctt cgtcttaatt
      301 ggcgaccttg ccctcaccat cgtttcggtg ttgggcagtt ttgcgttacg gctggatgtg
      361 agcgaattac cgttttattt ccctgccgca ctggtaatgt gtgcggtgac gttgcttgtc
      421 aaaattccaa cctattattt cttcggtctc tatcgtcgtt tatgggtgta tgccagcacg
      481 aatgaattac gcctcatcac ctttgctgtt accaccgctt ctgtattggc atcgggcgtg
      541 atgttcctgc tcatcggctt tgacttgatc aagccgggca tgccgcgctc cgctctcggc
      601 attgactggc ttatctcgct tatccttatt ggtggctcgc gttttgttct gcgcatcctt
      661 gctgaacaat cttcggcgca agccaatgcc aatactcgcc acgccctcat cctcggcgca
      721 ggcgacgcgg gtgcgctcgt cgtgcgtgag ttacagaaga cctcccaact taatctcatt
      781 cctgttggct ttctcgacga tgaccccgcc aagcagaatc acaacatcta cggtgtgacc
      841 gttatcggta agatcgatca acttgcaaat acgctcgatt ccaagcaggt gaacgaagtc
      901 atcatcgcca ttccctccgc accggggcat atcatccgcc tcgtcaacga tgaatgccgc
      961 aaacgcggaa tcacctcacg catcatcccc ggcttttacg aactgatcgg tggcaaggtc
     1021 agcgtcaacc gtctgcgcga agtggacatc accgacctcc tacgccgcga ccccgccaaa
     1081 attgatgacc gtctcattgg cgctactctg ggcggcaaac gcatcctcgt caccggcgca
     1141 ggcggctcga tcggcagtga actctgtcgt cagcttgcgc gttggaatcc gaccgaactc
     1201 gttttgctcg ggcatggcga gaacagcatc tttgaagcct tgcaagaact caagaacgat
     1261 tttcctgcgc tgaatcttca agctgtcatt gcggatgttc gcgatctaaa ccgcattgaa
     1321 aaagttttca atcaacacaa accgcaagtc gtttttcacg cggcggcgca caagcatgtt
     1381 ccgctcatgg aagctaatgt cgaagaggcg gtcacgaaca acgtgctcgg cacgaagaac
     1441 gtcgtcgaaa cagcggtcag gcacggggta gaaagactcg tgctcatctc caccgataaa
     1501 gcagttcgtc ccgtcagcgt gatgggcgcg acaaaacgtc tcgccgaact cgccgtcctc
     1561 gatgcggcac agcaacacaa cttgccttat tctgttgtgc gttttggtaa tgtgcttggt
     1621 agtcgcggca gcgtagtgcg cacgttcaaa aatcaaattg cgcgcggtgg tcccgttacc
     1681 atcacgcatc ctgaaatgta tcgctacttc atgatcatcc ccgaagccgt gcatcttgtt
     1741 ttgcaagccg catgcatggg cacgggcggc gaggtcttca tgctcaacat gggcgagcag
     1801 gttcgcatcc tcgaccttgc cgaagacctc attcgtcttt ccggtcttga gccatatcgt
     1861 gatattgata ttcaaaccac cggcattcgt cctggcgaaa aactgcgtga ggatttgctc
     1921 gaagacggcg tggactttga acgcacccaa cactcggaaa ttttccgcat
//
LOCUS       DAOQJE010000749         1961 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_47862_length_1961_cov_5.282267, whole genome shotgun sequence.
ACCESSION   DAOQJE010000749 DAOQJE010000000
VERSION     DAOQJE010000749.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1961)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1961)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1961
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47862_length_1961_cov_5.282267"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..439)
                     /locus_tag="PLT08_16055"
     CDS             complement(<1..439)
                     /locus_tag="PLT08_16055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE06040.1"
                     /translation="MNKSIFSKFFSAILIVMLALGALPITPASAAACTFTATANGVWN
                     APGTWTSVGVACSTYPGQTFAGDTVIIPNTRTVTLNITPAQSIASLTVGGGTSGTLTL
                     GDNNTDRTLTVTGTVTVSAGATLNSAGNGGNQLTIGGNLANAGT"
     gene            complement(496..753)
                     /locus_tag="PLT08_16060"
     CDS             complement(496..753)
                     /locus_tag="PLT08_16060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE06041.1"
                     /translation="MVLLRKDSQHIGKSIVQEYAQRLNMSCDQFIGEMRKLGCSEPTA
                     LKIWRGEYETFEDFNDNNVQLSNLRKAAVVLRVGTGSLLPD"
     gene            947..1432
                     /locus_tag="PLT08_16065"
     CDS             947..1432
                     /locus_tag="PLT08_16065"
                     /inference="COORDINATES: protein motif:HMM:NF014517.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CinA family protein"
                     /protein_id="HNE06042.1"
                     /translation="MDSQLELRIGMILSERKQSLVTAESCTGGLIASRITDVAGSSAY
                     FLGGIVAYSYEVKAALLGVSWDTLNNKGAVSRETVIEMARGARNILKADIAVSVSGIA
                     GPGGGTPDKPVGTVWVGLATPSGEEARHFVWDGDRVRNKYLSSEAALQFVLDYLEGNV
                     A"
     gene            1429..>1961
                     /locus_tag="PLT08_16070"
     CDS             1429..>1961
                     /locus_tag="PLT08_16070"
                     /inference="COORDINATES: protein motif:HMM:NF013232.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE06043.1"
                     /translation="MKTVVMISANAEWRSVKKIFPALVIERGPYGETAALTLKGFHLK
                     LFHSGWGKVASAGAMQYVIDHEQPDLIINLGTCGGFEGLIHQGDVVLVEKTFIYDIVE
                     LMGDPDVMEYYASSLDLSWLPDPLPHPAQRGTLASADSDLQPAQIAYLKSLGAIAGDW
                     ETGALAWVANKNAARLL"
BASE COUNT          499 a          412 c          515 g          535 t
ORIGIN      
        1 aggtacctgc atttgcaaga ttgccaccaa tggtgagctg gttgccacca ttgcctgcgc
       61 tattaagagt tgcacctgca cttaccgtta cagtgcctgt tacagttagt gttctgtcgg
      121 tattattatc gccaagagtc aacgtgccac tggttccgcc gcctactgtt agactggcaa
      181 tggattgagc aggggtgatg tttaaggtca ctgtgcgcgt gttggggata ataacagtat
      241 cacccgcaaa agtttgccct ggataggtgg aacaggcaac gccaacgctt gtccaagttc
      301 ccggtgcatt ccagacacca ttggcagttg ctgtaaaggt acaagcagct gcgcttgctg
      361 gtgtgatggg caatgccccc agtgccagca tcacaatcaa aattgcagaa aagaactttg
      421 agaaaataga tttgttcatg gttggtttcc cttgctgtgg ttggagtgga tgaataaaaa
      481 ataaaaataa tctgcctaat caggcaataa tgacccggtg ccaactctta aaacgacggc
      541 ggcttttcgt aaattactca attgcacatt gttatcgttg aagtcttcga aggtttcata
      601 ttccccgcgc caaatcttta gggcagtggg ttcactgcac cctagctttc gcatttcccc
      661 aataaattga tcacaagaca tgttaagtcg ttgtgcatat tcttgcacaa tgcttttccc
      721 tatatgttgg gagtcttttc gcaacaaaac cattactttg ctccatatcg gacaaaatgg
      781 gtttaaaaat gtatttttaa actgaggtaa aaaaatatct ggtttcccag tgtccttaca
      841 ttcctgtaag gtcgattaat agtattctaa tgtaaaagta ccaagagtca atacattttt
      901 gaaaggaaaa tcggctgata caatactgaa gtttgctagg agttcaatgg acagtcaatt
      961 agaattgcgt ataggcatga tattaagtga gcggaagcaa tctcttgtaa ctgccgagtc
     1021 ttgcacgggc gggttgattg ccagtcgcat cacggatgta gccgggtcat ccgcttattt
     1081 tttgggcggc atcgttgcgt attcgtatga ggtgaaggcg gcgcttcttg gcgtttcatg
     1141 ggatacgtta aataataaag gtgcagtctc aagagagacc gtcatcgaaa tggcgcgcgg
     1201 agctagaaat atattgaaag cagatattgc ggtgagtgtc agtggtattg cgggtccggg
     1261 tgggggcacg cccgataaac ccgtaggaac agtttgggtt gggctggcaa ctccgagcgg
     1321 ggaagaggcg cggcacttcg tctgggatgg tgaccgagtg cgcaacaaat atctttcaag
     1381 cgaagccgca ttgcaatttg tcctcgatta tttggaaggg aatgtagcat gaaaacagtt
     1441 gttatgatct ctgccaatgc cgagtggagg tccgtcaaga aaatatttcc ggcgcttgtg
     1501 atagagcgtg gaccttatgg cgaaacggcg gcgttaactc tcaaaggatt tcatctcaag
     1561 ttgtttcata gtggttgggg caaagtagct tcagcggggg caatgcaata tgtgatcgac
     1621 catgaacagc cagacttaat tattaacctt ggtacttgtg gcggctttga aggtctgatt
     1681 catcaagggg atgtggtgtt ggtggagaag acatttattt atgacattgt cgaattgatg
     1741 ggcgaccccg atgtgatgga atactacgcc tcttccctcg acctgagttg gttgccagac
     1801 cctttgcctc atcctgccca gcgcggaacg ctcgcctctg ccgacagtga cttgcaaccc
     1861 gcgcagattg cgtacctcaa atcattgggc gccattgccg gtgattggga aacaggcgcg
     1921 ttggcatggg tggcaaacaa aaacgccgcg cgtttgttaa t
//
LOCUS       DAOQJE010000750         1960 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_47921_length_1960_cov_3.990551, whole genome shotgun sequence.
ACCESSION   DAOQJE010000750 DAOQJE010000000
VERSION     DAOQJE010000750.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1960)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1960)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1960
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47921_length_1960_cov_3.990551"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..877
                     /locus_tag="PLT08_16075"
     CDS             <1..877
                     /locus_tag="PLT08_16075"
                     /inference="COORDINATES: protein motif:HMM:NF014717.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HNE06044.1"
                     /translation="GTTALSRATVVIGYMVAQNFMDPNIRPGQVPPPPPDLYDQQIQL
                     TLTKFDDKNNEIRKNYSLRVAGVLSESGSEADYSIYLPIEVVKSMNEWANNSTINYNK
                     TGYNEVLVRVENPDKTLEVRDAITALGFQANTSLDFVQGINNFYSILQIVLGGVGAIA
                     LLVAAIGIANTMAMSILERTREIGLMKAVGATNRDVLSLFLGEAAGIGFIGGLGGVLT
                     GWLLGQMLNVVAVVYLAQQAAQQGGVPPSVAIYTPIWLPVFVLAFSTFIGMLSGLYPA
                     LRAANMIPVLALKYE"
     gene            941..1282
                     /locus_tag="PLT08_16080"
     CDS             941..1282
                     /locus_tag="PLT08_16080"
                     /inference="COORDINATES: protein motif:HMM:NF018894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HNE06045.1"
                     /translation="MPEKRTVPRKKFNLYMRVLNDDTGEILGHMVEISSTGLRLETVG
                     PLPTNVDYYLRIELTPDLGSMPFIIFVARSKWCKIDTIQPNLFQVGFEVVEMVPEDRE
                     AFLRIMQKYAM"
     gene            complement(1300..>1960)
                     /locus_tag="PLT08_16085"
     CDS             complement(1300..>1960)
                     /locus_tag="PLT08_16085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="WD40 repeat domain-containing protein"
                     /protein_id="HNE06046.1"
                     /translation="KVLRSVPIPDLAPGDVWIKWVQLNKTIIVSSEADAEVGIPLYVV
                     NRESGKIVAKMGFPIIPTAYLIIHPNKPLMYFNSFKDPYFAVWDMEKYKITNKIEITP
                     RTDRMLFFKNKNEVWIASSLDGSILRYNADTLKNTGFIKSNIGDRTLSIDYKRNLLFT
                     GNFMNGRLTVIDLSTQKKVASYYLGPWIRTIALNTEKGIAYVSTVRGLFTIRYIQDVD
                     K"
BASE COUNT          549 a          376 c          435 g          600 t
ORIGIN      
        1 agggactacg gcattgagtc gtgctaccgt ggtcattggg tatatggtgg cgcaaaactt
       61 tatggaccca aacattcgac cagggcaagt cccaccacca cccccggacc tatatgacca
      121 gcagatccaa ttaaccttga cgaagtttga tgataaaaat aatgaaatcc gcaaaaatta
      181 cagtttgcgg gttgctggtg ttctttccga gtctggctct gaagcggatt attccatcta
      241 tctcccaatt gaagtggtta agtccatgaa tgaatgggcg aataattcga ccataaatta
      301 caacaagaca ggatacaacg aggttttggt acgagtagaa aaccctgata aaacccttga
      361 agtacgtgat gccatcaccg ccttagggtt ccaggcaaat acttcattag attttgtgca
      421 aggcatcaat aacttctatt cgatcttgca gatcgtgctt ggtggggtgg gtgccattgc
      481 tttgcttgtt gctgccattg ggattgcaaa tacgatggca atgtctattc tggagcgcac
      541 tcgggaaatc ggattaatga aagcggttgg cgctactaac cgtgatgttc ttagtctctt
      601 tctaggcgaa gcggcgggga ttggttttat tggcggttta ggcggtgtgt tgacaggatg
      661 gttattgggg caaatgttaa acgtagtcgc cgtggtatat cttgcccaac aggctgccca
      721 gcaaggtggc gttccgccca gtgtggcgat ttacacacct atttggcttc cagtctttgt
      781 tttagcgttt tctactttta ttggcatgtt atctgggctt tacccggcct tacgggcagc
      841 taacatgatt ccagttttag cattgaaata cgaatgaaaa acactttctt caattgtata
      901 attagaagaa gatattaagt ggaaagtaga ggtaaatcat atgccagaga aacgaactgt
      961 tccacgcaag aaatttaact tgtacatgcg tgtcttgaac gatgatacag gggaaatcct
     1021 aggacacatg gtagagataa gttcaacagg gctaaggctt gagacggtgg gtcctttacc
     1081 tacgaatgtt gattactatc tgcgtattga acttacacca gatttaggtt ccatgccgtt
     1141 tatcattttt gttgcacgta gtaaatggtg caagatcgat acgatccaac caaatctgtt
     1201 tcaagttggt tttgaggttg ttgaaatggt accagaagac cgtgaagcat ttttgcgcat
     1261 tatgcagaag tatgcgatgt agctcctaca ttcttagctc tatttatcta catcttgaat
     1321 atagcgaata gtaaacagac ctcttacagt tgaaacgtac gctatgcctt tttcagtatt
     1381 tagggcaatt gtccgtatcc atgggccaag gtaatagctt gcaactttct tctgcgtaga
     1441 taagtcaata actgtaaggc gcccgttcat aaaattgccg gtaaataata aatttctttt
     1501 gtagtctata cttaaagtcc ggtcgccaat attacttttg ataaaaccag tgttttttaa
     1561 cgtgtcggca ttgtagcgta agatagaacc atcaagcgat gatgcaatcc atacttcatt
     1621 tttgttttta aagaagagca tacgatcagt acgaggagtt atttcaattt tatttgtaat
     1681 tttatatttt tccatatccc agactgcgaa atacggatcc ttgaatgagt tgaaatacat
     1741 taaaggttta tttggatgaa taattagata tgcggtgggg attattggga aacccatttt
     1801 agcaacaatc tttccacttt ctcgatttac aacatataac ggaattccga cctctgcatc
     1861 tgcttcagaa gaaacaataa tagttttgtt caattgaacc cattttatcc atacatctcc
     1921 aggggctaag tctggaatgg gcacagatcg aagaaccttc
//
LOCUS       DAOQJE010000751         1958 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_48012_length_1958_cov_4.679453, whole genome shotgun sequence.
ACCESSION   DAOQJE010000751 DAOQJE010000000
VERSION     DAOQJE010000751.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1958)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1958)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1958
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48012_length_1958_cov_4.679453"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..232)
                     /locus_tag="PLT08_16090"
     CDS             complement(<1..232)
                     /locus_tag="PLT08_16090"
                     /inference="COORDINATES: protein motif:HMM:NF019928.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC domain-containing protein"
                     /protein_id="HNE06047.1"
                     /translation="MPSKNKTTVNVEPDKQELYIIREFEAPRELVFRAHTDPNLFVQW
                     IGPHGYEMILKEFEPVNGGRYRYIHKDPQGNEY"
     gene            complement(216..551)
                     /locus_tag="PLT08_16095"
     CDS             complement(216..551)
                     /locus_tag="PLT08_16095"
                     /inference="COORDINATES: protein motif:HMM:NF033788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HNE06048.1"
                     /translation="MRRDVFQAIADPNRRAILALLSQQRLTLNGVAENFRISRPAVSR
                     HIKILKECGLVVVVPQGRERFVEARFDKLEEVSRWVEQYRQVWEEKFNRLDGLLVQMK
                     KEKNDALEK"
     gene            668..1348
                     /locus_tag="PLT08_16100"
     CDS             668..1348
                     /locus_tag="PLT08_16100"
                     /EC_number="2.1.1.100"
                     /EC_number="2.1.1.334"
                     /inference="COORDINATES: protein motif:HMM:NF018649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenylcysteine carboxylmethyltransferase
                     family protein"
                     /protein_id="HNE06049.1"
                     /translation="MKSLPRYFLALLAGSVIFIGLPLLGWGLGVIPAFFENPARIAFV
                     FVLFGLQVFSLVYNPQVGRNQENRKSDAPSSKLDLILIQIFSLAVVILAPFSDGRSIG
                     ILHVENTIRYIGLFIAIPGFVLMQAGEKYLAKQFSIEVTLQKDHKLIQSGPYKVIRHP
                     RYLGILIFFTGISLTFRSLLGIFIVLALAGVLIWRVFAEEKMMHAEFGKEWEEYQSKT
                     WRLIPYIF"
     gene            complement(1384..>1958)
                     /locus_tag="PLT08_16105"
     CDS             complement(1384..>1958)
                     /locus_tag="PLT08_16105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004664 - prephenate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009094 - L-phenylalanine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="prephenate dehydratase domain-containing
                     protein"
                     /protein_id="HNE06050.1"
                     /translation="VRHCLIANPGVKKEDIKKAISHPQALGQCAAYLRNHGIKAEQVY
                     DTAGSVKMLKESGAKDVAAIASRRAAELYGMQILEEGIEDNPENFTRFLAVGREAVVP
                     ESDAKTSIVFTLKNVPGSLFKAMSVFALRDIDLTKIESRPLQGKPWEYLFYIDLIGSV
                     QNETVQRALDHLGEYAVMLRVLGSYPRFKG"
BASE COUNT          491 a          515 c          403 g          549 t
ORIGIN      
        1 catattcatt tccctgcggg tctttgtgaa tataacgata cctgccacca ttgaccggct
       61 caaactcttt gagaatcatc tcatacccat gcggaccaat ccattggacg aaaaggtttg
      121 ggtcggtgtg agcacgaaac accaactcgc gcggcgcctc gaattcacgg atgatataca
      181 gttcctgctt atcaggctcc acattgacag ttgttttatt tttcgagggc atcgttcttc
      241 tcctttttca tttgcactaa tagaccgtcc aacctattaa atttctcttc ccacacctga
      301 cggtattgct ccacccagcg agacacttct tccaatttgt caaaacgcgc ttccacaaag
      361 cgttcgcgtc cctgcgggac gaccaccacc aacccacatt ccttcaatat cttgatatgg
      421 cgcgatactg ccggacggct aatgcgaaaa ttctcggcga cgccgttcaa ggtcaatctc
      481 tgttgtgaaa gcaacgccaa aatagcgcgg cggttggggt ctgcaatggc ttgaaatacg
      541 tctcgtctca tagattccta tatgtaacca aatggttact atttttcatg tattttagaa
      601 cacattttct gttttgtcaa gtaggaatca tttatactgt cgtcaaatct aaaaggaaca
      661 aaacaatatg aaatcactcc cccgctattt ccttgccctg ctcgcaggct ctgtcatttt
      721 catcggtctc cccttgctcg gttgggggct cggagttatc ccagctttct ttgaaaaccc
      781 agcccgcatt gcttttgtct tcgtgctatt cggcttgcaa gttttttcct tggtctacaa
      841 cccacaagtg ggacggaatc aagaaaaccg aaagagtgat gcccccagca gcaaactaga
      901 cttgatcttg attcaaattt tcagcctggc agtcgtcatc ctcgccccat tttcagacgg
      961 acgttccatt ggcatactac atgtagaaaa taccattcga tatattgggt tgttcatcgc
     1021 catccccggt ttcgtattaa tgcaggcagg cgaaaaatat cttgccaagc agttcagcat
     1081 cgaagtcact ctgcaaaaag accacaaact catccaaagc ggaccctaca aagtgatccg
     1141 tcatccgcgc tatttgggca ttttgatatt cttcacgggc atctcgctga ccttccgctc
     1201 tttactcggc atcttcattg ttcttgctct ggcaggcgta ttgatctggc gcgtctttgc
     1261 agaagaaaaa atgatgcatg cagaatttgg caaagaatgg gaagagtatc aaagcaaaac
     1321 ctggagactc attccataca tcttctaaat aaaaaatctc cgcataaagc ggagattttt
     1381 ttattaccct ttgaaacgcg ggtatgaacc caacactcgc agcatcacgg cgtattcgcc
     1441 taaatggtcg agcgcgcgtt gaacggtttc gttttgtaca gaaccgatca ggtcaatata
     1501 aaaaagatat tcccacggct tgccttgtaa tgggcgcgat tcaattttgg tcaggtcaat
     1561 atctcgcaac gcaaaaacag acatcgcctt gaataacgaa ccggggacat ttttgagtgt
     1621 gaatacgatg gaagtcttcg cgtcagactc agggacaact gcttctcgtc cgacagcgag
     1681 aaaacgcgta aagttttccg ggttgtcttc aataccttct tctagaattt gcatgccata
     1741 caactctgcc gcacgcctag atgcaattgc cgccacatct tttgcgcctg attccttgag
     1801 catcttcaca ctccccgccg tatcgtagac ttgttccgct ttgatgccat gattacgcag
     1861 atatgccgcg cactgaccta acgcctgcgg gtggctgatg gctttcttaa tgtcttcttt
     1921 cttcacaccg gggtttgcga tcagacaatg ccgcacgc
//
LOCUS       DAOQJE010000752         1957 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_48069_length_1957_cov_3.241851, whole genome shotgun sequence.
ACCESSION   DAOQJE010000752 DAOQJE010000000
VERSION     DAOQJE010000752.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1957)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1957)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1957
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48069_length_1957_cov_3.241851"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..665
                     /locus_tag="PLT08_16110"
     CDS             <1..665
                     /locus_tag="PLT08_16110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE06051.1"
                     /translation="VNVNACLLFVDVDPTYQSILPNLTAVLTGTGLGGALTGAGDQGF
                     STQGLPILSLGVVSSGSAQGRLELDPSVQQPYYVIPSEAQRPRMVCQTLLQDVVVMRL
                     GNFPLVETDPAAAPTQTPDAAADQQTAPATPDIVTLIVSPQDSITLSYLVYTNAKISL
                     TLRNPSDQARLATEASTLQFLLSQYNIPVPAKLPYTMQPAITTLSSPVLSNDAAAQQP
                     QQ"
     gene            687..1919
                     /locus_tag="PLT08_16115"
     CDS             687..1919
                     /locus_tag="PLT08_16115"
                     /inference="COORDINATES: protein motif:HMM:NF022077.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNE06052.1"
                     /translation="MADKIRVLIVDDIAETRENVRKLLQFEADIEVVGTARTGKEGIQ
                     IAQELNPDVVLMDINMPDVDGITATEQIRQHSAHIQVVILSVQGDQNYMRRAMLAGAR
                     DFLTKPPMGDELISAVKRAGEMAQSEREKGSKQISLSASLGTSSIGGFATSQGKIILV
                     YSPKGGTGCTTIAVNLAIALHNEDTRAVLIDGNLQFGDVAIFVNEQGKNTVLDIAPRV
                     DDLDADVVDEILIKHEASGIRILAAPQRPEMAEKISSDQFTKVLQFMQKIYSYIIVDT
                     SSLLTDVTLSAIDLSDIVVLVATQEIPSIKNSRLILDLLNSMGINKGRVVFAMNRFDK
                     RIAIAPERISENLKHEISAVIPLDEKIAISAVNRGVPFMLDNKSQPISRGILALAESV
                     RSRIIALDSETEALPAKR"
BASE COUNT          559 a          378 c          417 g          603 t
ORIGIN      
        1 atgtcaatgt aaatgcttgt ttactttttg tggatgtaga tcctacgtat caatccatac
       61 ttccaaactt aacggcagtt ttgacaggta ctggtttagg aggcgcatta acaggcgctg
      121 gtgaccaggg cttttcaaca caaggtctgc caattctttc tcttggtgtt gtttcttcag
      181 gatctgcaca aggacgcctt gagttggatc catcagtcca acaaccatat tatgtgattc
      241 cttctgaagc acagcgccct cgtatggttt gtcagacgtt attgcaagat gttgttgtta
      301 tgcgactagg gaatttccca cttgttgaaa ctgaccctgc tgcagcacct acacaaacac
      361 cagacgcagc agcagaccaa caaactgcgc ctgctacccc agatattgtt acattaatag
      421 tgtcacccca agattctata accttgtctt atttagtgta cacaaatgca aagatttcgc
      481 taacgctgcg aaacccatct gatcaggcac gtttagctac tgaggcgtca acattacagt
      541 tcttacttag tcaatataat atccctgttc cagcaaaatt gccatataca atgcagcctg
      601 ctataactac attgtcttct cctgttttat caaatgatgc tgcggctcaa cagccgcaac
      661 agtaaaactt attggatttc ttgaatatgg ctgataaaat acgtgtcctt atagttgacg
      721 acattgctga aacccgtgaa aatgtccgaa aactgcttca atttgaagct gacattgaag
      781 tagttggtac ggctaggact ggaaaagaag gtatccaaat tgctcaggag ttgaatccag
      841 atgttgttct aatggatatt aacatgcctg atgttgatgg tattactgca actgaacaga
      901 tacgtcagca ttcagcacat attcaagttg ttattttatc tgtgcaaggc gatcagaact
      961 atatgcggcg tgccatgtta gctggagcga gggacttctt aacaaaacct cctatgggtg
     1021 atgaactcat ttctgctgta aaacgagcag gtgaaatggc gcaatcagag cgagaaaaag
     1081 gctctaagca aatatcactt tctgcatcgc ttgggacttc atctattggt ggttttgcta
     1141 cttcccaggg caagataatt ttggtgtata gccctaaagg tggaactggt tgtacaacta
     1201 ttgctgtaaa ccttgctatc gcacttcata atgaggatac gagagcagtg cttatagatg
     1261 gtaatttgca atttggcgat gttgctatat ttgtaaatga gcaagggaaa aatacagttc
     1321 tcgatattgc gcctcgggta gatgatcttg atgccgatgt tgtagatgaa atcttaataa
     1381 agcatgaagc atctggaata cggattttag ctgctcctca acgcccagag atggcagaga
     1441 agatttcttc tgaccagttt acaaaagttt tgcaatttat gcaaaagatt tattcatata
     1501 taattgtaga tacatcttct cttcttactg atgttacact ttcggctatc gacttaagcg
     1561 atatcgtagt attggttgct acacaagaaa ttccttctat caagaattct agattgattc
     1621 ttgatttgtt gaatagtatg gggattaata aaggacgtgt tgtgtttgct atgaaccgtt
     1681 ttgataagcg cattgcaatt gctcctgaac gaattagtga aaatctaaaa catgaaattt
     1741 ctgcagttat tccattagat gaaaaaattg ctatatctgc ggttaaccgt ggtgtgccat
     1801 ttatgttaga taataaatca cagccaatca gtagagggat attggcgctt gcagagtctg
     1861 tgcggtctcg tatcattgct ttggatagcg aaactgaagc gctccctgct aaaagataag
     1921 gagtttttat gtctttacta aggcgtattg agcaagg
//
LOCUS       DAOQJE010000753         1936 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_49047_length_1936_cov_4.323764, whole genome shotgun sequence.
ACCESSION   DAOQJE010000753 DAOQJE010000000
VERSION     DAOQJE010000753.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1936)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1936)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1936
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49047_length_1936_cov_4.323764"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..456
                     /locus_tag="PLT08_16120"
     CDS             <1..456
                     /locus_tag="PLT08_16120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE06053.1"
                     /translation="LLAEGGPKWKEKGPFNLGGLSKPNAFLAVIFGIVLAISGFFPPN
                     EKVFYFTIVFVVALLAFWSKKYAPISIVVAVIGFALTLLPVNEANVFHFLVPDRTTAI
                     IAIVVSVVGTVITFVTGGEDARFEGVPEGDKIKERQAKIAEIEKQYGEA"
     gene            525..>1936
                     /gene="glpK"
                     /locus_tag="PLT08_16125"
     CDS             525..>1936
                     /gene="glpK"
                     /locus_tag="PLT08_16125"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: protein motif:HMM:NF000756.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HNE06054.1"
                     /translation="MKYILGIDQGTTQTTAVIVNEFGEMIEKNSAQLPARFPQAGWVE
                     QEPEDIVRTVKEACAPLLSKYKISAVGFDNQGETFIVWDAETGNAVTPAIVWQDTRGQ
                     AVCDTLASNVDLNWLRQTTGLLLDSYFSAPKLKWVFENHPEIRQRAHEGKLKFGTTET
                     WVIWKLSGGKLHVTDPSTASRTLLFDMNTFQWDEKLLQLFDVPKSMLPEVKPSAGHIG
                     DVDFGNGKPLPLHALLVDQQAALFGQACFKAGEMKCSFGTGSFLLMNIGDKPKLSNNG
                     LLTTVGWNFNGQTAYAFDGGIFVTGSAVQWLRDNLKFIPDSASSHNAANNSKDMGVVV
                     IPALQGLAAPHWRTDVQGAMFGLNRSTTSDDIVRATLDGIACRVYEVVTAMSQDSGTP
                     PPYLKVDGGPAGNPYLMQMISDLLNLEVRVSASVEATAIGIANLAGVSALGTSFEQLA
                     GNWKAERVYTPSMKEEERRK"
BASE COUNT          493 a          508 c          479 g          456 t
ORIGIN      
        1 ttgctcgctg aaggcggacc taaatggaaa gaaaagggtc cattcaacct tggtggtctc
       61 tccaaaccga atgccttcct tgccgtgatc ttcggtattg tcttggcaat cagcggtttc
      121 ttcccgccca atgaaaaagt cttctacttc accatcgtat tcgtcgtagc cttgcttgct
      181 ttctggtcga agaagtatgc gcccatcagt attgtggtcg ccgtgattgg ctttgccttg
      241 acccttcttc cggtcaatga agcaaatgtc ttccacttcc tcgttcccga tcgcaccacc
      301 gcgattatcg ccatcgtcgt tagtgtggta ggaacggtca tcacctttgt aaccggtggt
      361 gaagatgccc gcttcgaagg cgtacccgaa ggcgacaaaa tcaaggaacg ccaagccaag
      421 atcgccgaga tcgaaaagca atacggcgaa gcctaattct gtttcaataa aagagcggta
      481 tctcgtatga gataccgctc ttttaaacca acgacggagg caacatgaaa tacatacttg
      541 gtattgatca aggcaccaca caaaccacag ccgtcatcgt caatgaattt ggcgagatga
      601 tcgaaaagaa ttcggcgcag ctcccggctc ggttcccgca ggcaggctgg gtcgagcaag
      661 agccggagga cattgtccgc accgtcaagg aagcctgcgc tccactttta tccaagtaca
      721 aaatctccgc cgtcggtttt gataatcaag gcgaaacctt cattgtttgg gatgcagaaa
      781 ctgggaatgc cgtcacgcct gccatcgtct ggcaagatac acgcggacag gcggtttgtg
      841 acacgctcgc ctcaaacgtg gatttgaact ggttgcgcca aacaaccggg ttgctgcttg
      901 acagttattt ctccgcccca aaactcaagt gggtctttga aaatcatcca gagatccggc
      961 aacgcgcaca cgaaggcaag ttgaaatttg gcaccaccga aacctgggtg atctggaaac
     1021 tcagcggcgg gaaattacac gtcaccgacc cttccaccgc atcacgcacc ctattgttcg
     1081 atatgaacac ctttcagtgg gatgaaaaat tactgcaatt atttgatgta cctaaaagca
     1141 tgctgcctga agtgaaacct tcggcagggc atattggcga tgtagatttt ggcaacggca
     1201 agcctttacc gttacatgca ttattagtag accaacaagc ggctctcttt gggcaagctt
     1261 gtttcaaagc tggagaaatg aaatgctcgt ttggtacggg cagtttctta ttgatgaata
     1321 tcggcgataa accaaaactc tcgaacaatg gcttgcttac caccgtcggt tggaatttca
     1381 atgggcaaac cgcatatgcc ttcgacggcg gcatcttcgt cacaggctcg gctgtgcaat
     1441 ggctgcgcga taatctcaaa tttattcctg actcagcgtc cagccataat gccgctaaca
     1501 actcgaaaga tatgggcgtg gtcgtcatcc ccgccttgca aggactcgcc gccccgcatt
     1561 ggcgcacaga cgttcaaggc gccatgtttg gcttgaaccg cagcaccaca tctgatgaca
     1621 ttgtccgcgc aacattggat ggcatcgcct gccgtgtata tgaagtggtc acagccatgt
     1681 cacaagatag cggcactccc ccgccctacc tcaaagtgga cggtggtccg gcgggcaacc
     1741 cgtacctgat gcaaatgatc tcagacctgc tcaaccttga agtgcgtgtc tctgcatccg
     1801 ttgaagcaac cgctattggc attgcaaatc tcgcaggcgt ttcggcattg ggaacttcct
     1861 ttgagcaact ggcaggaaat tggaaagccg agagggttta tacgccgagt atgaaggaag
     1921 aggaaagaag aaagaa
//
LOCUS       DAOQJE010000754         1933 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_49183_length_1933_cov_7.048456, whole genome shotgun sequence.
ACCESSION   DAOQJE010000754 DAOQJE010000000
VERSION     DAOQJE010000754.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1933)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1933)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1933
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49183_length_1933_cov_7.048456"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..473
                     /locus_tag="PLT08_16130"
     CDS             <1..473
                     /locus_tag="PLT08_16130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE06055.1"
                     /translation="RIKKSRRRIHTPAGYSQKPANYFFLLDFEDVFGVLEVLEVEDGV
                     VFFVVDGLAPADLAFAGALVLVAAGLAAAFAALESGVVFTEVMAGNSSSTTGLVAACF
                     AASRQLGKKSSIRWREMELARRQRGQCASWWFWCFSSMVISAVNISARPTVNGV"
     gene            complement(590..1588)
                     /locus_tag="PLT08_16135"
     CDS             complement(590..1588)
                     /locus_tag="PLT08_16135"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNE06056.1"
                     /translation="MLTEKPLLDLNLKLRNTTRADVNAVADLIYAVCEADGDTTVATS
                     VEELESQWRSEKFNPETDSHVIETSDGRVIAYGEFYNHNRHIDLGADIYIHPEFKGKG
                     LFTPVLAWIETRAYEELKLAEPDLRVFIRSTMDGKDMEARQAHEAAGYTAVRYAWRMD
                     IHLTEAPPTPVFPEGVELRPFDKEAHARLLMDAENEAFSEHWGSHASEFEEWAYRKLE
                     NPDFDPTLWLIAWDGDQIAGFSQNRFRMGVGWVSTLGVRKPWRKTGLGLALLQYSFGE
                     FYRRGMKTVGLGVDASNKTGATRLYQRAGMQIASEFVTFEKEIRPGRDLEDAKQRE"
     gene            complement(1602..>1933)
                     /locus_tag="PLT08_16140"
     CDS             complement(1602..>1933)
                     /locus_tag="PLT08_16140"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HNE06057.1"
                     /translation="RLIGKALIEYVDWSHGNGYLRLGIGEAEARRKGYGSQALNLLLQ
                     YAFCELNLYRVTAVVPAYNRGALRLFQKFGFIEESRRPQALHHADTFWDVIGFGLLNS
                     EYREQCL"
BASE COUNT          441 a          486 c          499 g          507 t
ORIGIN      
        1 tgcggataaa aaaatcccgc aggcggatac acacgcctgc gggttattca caaaaaccag
       61 caaattactt cttcttgcta gacttcgaag acgttttcgg cgtcttggaa gttttggaag
      121 tagaagatgg cgtagtcttt tttgttgtcg atggcttggc gccagctgat ttagcttttg
      181 ccggagcttt ggttttggtg gctgccggtt tggcagcagc tttcgccgct ttggagagcg
      241 gtgttgtttt tacagaagta atggcgggca attcatcatc caccaccggt ttggtggctg
      301 cctgtttcgc agcctcacgc caattgggaa agaagagttc catacgctgg cgcgaaatgg
      361 agttggcacg acgacagcgc gggcagtgcg catcgtggtg gttctggtgc ttctcttcca
      421 tggtgataag tgcggttaac atctccgcac gacctacggt gaatggggtt tgacaataaa
      481 gacaacgcag atttagcatg gtagtgccct ttcgtggatt gtactcatag taccgcttga
      541 ttctacaccg aatttttcta ggttgcgaat caagtttggg caaaaatgct tattcgcgct
      601 gttttgcgtc ctccaaatcc ctgccggggc ggatttcctt ctcaaaagtg acgaattcgc
      661 tggcaatttg catcccggca cgctgataaa ggcgggttgc cccggtcttg ttggaggcat
      721 ctacgcccaa cccgaccgtt ttcatgcccc ttcggtaaaa ctcgccgaag gagtattgta
      781 agagagccag ccccagccca gttttgcgcc aaggcttgcg gacgcccaaa gtgcttaccc
      841 atcccactcc catgcggaaa cggttctgtg aaaaaccggc gatctggtcg ccgtcccagg
      901 caatcagcca caaggtgggg tcaaaatctg ggttttccaa tttgcggtat gcccattctt
      961 caaattcaga agcatgactg ccccaatgct cactgaatgc ttcattttcg gcatccatca
     1021 aaaggcgggc gtgagcttct ttatcgaacg ggcgtagttc aacaccctct gggaagactg
     1081 gtgtaggcgg cgcttcggtg aggtggatat ccattcgcca cgcatagcgg acggcggtat
     1141 agccagcggc ttcgtgtgcc tgacgtgctt ccatgtcttt gccatccatc gtgctacgga
     1201 taaaaacccg caggtctggt tcagcaagtt tcagctcttc ataggctcgt gtttcgatcc
     1261 aagccaagac gggcgtgaaa agccctttgc ctttgaactc tgggtggatg taaatatcgg
     1321 ctcccaggtc aatatgtcga ttgtggttgt aaaactcacc ataggcaatt actcgcccgt
     1381 cagacgtttc gatgacgtgg ctatcggttt cggggttgaa tttttcgcta cgccactggc
     1441 tttccaattc ttctacagag gtcgcaacgg ttgtgtcacc atcagcttca cacacggcat
     1501 agatcaagtc agctacagcg ttcacatctg cccgggtagt gttgcgcagt ttcaagttaa
     1561 ggtcaagaag cggtttttcg gtcaacatac tattttgcct tttataaaca ttgttctcgg
     1621 tattctgagt tcaataaacc aaatccaatc acatcccaaa acgtatcagc gtggtgcaaa
     1681 gcttgggggc ggcgggactc ttcgataaaa ccaaatttct ggaacagacg gagagcgcct
     1741 cggttataag caggaacaac agccgtcaca cgatataggt ttagctcgca gaacgcatat
     1801 tgcaacagca agttcaaagc ttgtgaaccg tagcccttgc ggcgtgcctc tgcttcgcca
     1861 atacctaaac ggagataacc attgccatgc gaccagtcca catattcaat gagggctttg
     1921 ccgatcagac ggt
//
LOCUS       DAOQJE010000755         1933 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_49191_length_1933_cov_5.192226, whole genome shotgun sequence.
ACCESSION   DAOQJE010000755 DAOQJE010000000
VERSION     DAOQJE010000755.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1933)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1933)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1933
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49191_length_1933_cov_5.192226"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..608
                     /locus_tag="PLT08_16145"
     CDS             <1..608
                     /locus_tag="PLT08_16145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE06058.1"
                     /translation="VRRMHKTEWLVKASGMLANEKTSEALESVAIYAAASSTSFGRAY
                     YQPRFNRVGEVIQERDPITGVSVSAKLESSSPAYHITYTAALNSGETFNGSETILGTT
                     IGLRGLGMPAPSKFTFKAADYEAEFTGTLTSELALSLFGNTKIRAYGSLDFKDNAGNA
                     GHLDLNRQGYVTLKINDQPTVTHALVRVMWLDMMSLSPQPL"
     gene            605..>1933
                     /locus_tag="PLT08_16150"
     CDS             605..>1933
                     /locus_tag="PLT08_16150"
                     /EC_number="4.1.99.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003904 - deoxyribodipyrimidine
                     photo-lyase activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribodipyrimidine photo-lyase"
                     /protein_id="HNE06059.1"
                     /translation="MTNIWWIRRDLRLTDNAALHSALEAGSVLPVFILDPAFDNLSER
                     RKHFLYEGLSALDKDLRKRNSYLVVRKGKPIDALRQLFDEVKAETIYAEEDYTPYSRK
                     RDEFIQKLLPLSLVQGQTVHHPKSVLKQDGRPYTVYTPYSKVWKAQLTKIKLHPAPEN
                     LNTPKSIRSEPLPAFTPSPIFPAGEAEALRRLNQFTQSIIKNYHDDRNRMDLDGTSSL
                     SPYIRFGMVGLRQAVHAARATHHADSKGAEIWLNELIWREFYIQILYHFPYVAKTAFN
                     RSLANIPWRNKSSEFEAWKRGETGVPVVDAAMRQLKETGWMHNRARMIVASFLVKDLL
                     IDWRWGEDWFMQNLLDGDIAPNNGGWQWTAGTGTDAAPYFRIFNPVLQSAKFDPNGDY
                     IRKWVPELRGLSAKQIHAPWEYDFKISGYPSEPIVDRDVVKERTLSAYSVS"
BASE COUNT          502 a          522 c          461 g          448 t
ORIGIN      
        1 ttgtgcgccg catgcacaaa acagagtggc tcgtgaaagc aagtgggatg ttagcgaatg
       61 aaaaaacaag cgaggcactt gagtccgttg ccatttacgc agccgcctcc tccacttctt
      121 ttgggcgggc gtattatcag ccacggttca atcgcgtagg agaggtgatt caagagcgcg
      181 accccatcac aggcgtatcg gtatctgcca agcttgaaag cagttcacct gcttatcaca
      241 ttacctacac ggcagccttg aacagcggcg aaaccttcaa tgggtctgaa accatcctcg
      301 gcactactat cgggttgcgc ggtttgggca tgcctgcccc atccaagttc actttcaaag
      361 ctgcagacta tgaagcagaa ttcacaggca cacttaccag tgaacttgcc ctttcccttt
      421 tcggcaatac caagattcgt gcttacggct cgctcgactt caaagataac gcagggaacg
      481 ccgggcatct tgacctaaac cgtcagggct acgtcacgct gaaaatcaac gatcaaccca
      541 ccgtcacaca cgctcttgtg cgcgtgatgt ggttggatat gatgagcctt tctccgcaac
      601 cgctatgaca aatatttggt ggattcgccg cgacctacgt ttgacagata atgctgcgct
      661 tcattctgca ctcgaagctg gttctgtctt gccggtcttt attctcgacc cggcattcga
      721 caacctatca gaacggcgca aacattttct gtatgaaggc ttatctgctt tagacaaaga
      781 cctgcgtaaa cgtaattcat atttagtcgt tcgcaagggc aaacccatcg acgcactccg
      841 ccaacttttc gacgaagtaa aagctgaaac catctatgcg gaagaagatt acacacccta
      901 ttctcgcaaa cgagacgaat ttattcaaaa gctgttgcct ttgagtttgg tccaagggca
      961 aaccgtacat caccctaaat cggtcctcaa gcaagatggc agaccttaca cggtgtacac
     1021 accctattcc aaagtatgga aagcacagct taccaagatc aaattacacc ctgcaccaga
     1081 aaatctgaat acacccaaaa gcattcgctc tgagccatta cctgctttca cgccaagtcc
     1141 cattttcccg gcaggcgaag cggaagcgct ccgccgcctt aaccaattta ctcaatcgat
     1201 catcaagaat taccacgacg accgcaaccg tatggacttg gatggaacct catctctctc
     1261 accatacatc cgctttggca tggttgggtt gcgtcaagcc gtgcatgctg cacgagctac
     1321 acatcacgca gattctaaag gcgcagaaat ctggctcaat gaactcatct ggcgtgagtt
     1381 ctacattcag attctgtatc acttcccgta tgttgctaaa accgccttca accgctcact
     1441 ggcgaacatt ccgtggcgca ataaatcgtc agagtttgaa gcgtggaaaa ggggcgaaac
     1501 aggcgtcccg gtggtagatg cggcgatgcg tcagctcaaa gaaacgggtt ggatgcacaa
     1561 ccgcgcgcgc atgatcgtcg catcgttcct tgtcaaagac ttgctcattg attggcgctg
     1621 gggcgaagat tggttcatgc aaaacctgct tgatggcgat atcgcaccta acaacggcgg
     1681 ctggcaatgg acggcgggaa cgggcaccga cgccgcgccg tattttcgta tttttaaccc
     1741 ggtgctgcaa agtgcgaagt tcgacccgaa cggcgattac attcgcaaat gggtgccaga
     1801 gttgcgcggg ttaagcgcga agcaaattca cgcgccgtgg gaatatgact tcaagatatc
     1861 gggttatcca tctgaaccga tcgtagatcg agacgtggta aaagagcgca cgttgagcgc
     1921 atatagtgtt tca
//
LOCUS       DAOQJE010000756         1929 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_49379_length_1929_cov_5.144610, whole genome shotgun sequence.
ACCESSION   DAOQJE010000756 DAOQJE010000000
VERSION     DAOQJE010000756.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1929)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1929)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1929
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49379_length_1929_cov_5.144610"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..199)
                     /locus_tag="PLT08_16155"
     CDS             complement(<1..199)
                     /locus_tag="PLT08_16155"
                     /inference="COORDINATES: protein motif:HMM:NF012904.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HNE06060.1"
                     /translation="MQLKGIYAPIVTPFNADESINYPVLEQLIEYLIANKIAGLVPGG
                     TTGEVYAFTEAERLDIFKFVKE"
     gene            complement(913..1284)
                     /locus_tag="PLT08_16160"
     CDS             complement(913..1284)
                     /locus_tag="PLT08_16160"
                     /inference="COORDINATES: protein motif:HMM:NF037832.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5615 family PIN-like protein"
                     /protein_id="HNE06061.1"
                     /translation="MGDLVTLRFLIDEDVPRSTTRVLRDAGFDVMNVHEAGLQGKSDD
                     LVFAFAQSENRLLITCDMGFSNILKFPPSENQGILVVRVPDSEPIDVFNREVLNAVHE
                     VGENLLHHLAIVEIGKVRLRG"
     gene            complement(1269..1496)
                     /locus_tag="PLT08_16165"
     CDS             complement(1269..1496)
                     /locus_tag="PLT08_16165"
                     /inference="COORDINATES: protein motif:HMM:NF016167.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF433 domain-containing protein"
                     /protein_id="HNE06062.1"
                     /translation="MFDLSLGITVDPSVRFGKPVIKGTRVPVDTVVARVASGMSIKDV
                     AEEYGITEKDVYNALHYAAIRLSEEQVWATS"
     gene            complement(1539..1847)
                     /locus_tag="PLT08_16170"
     CDS             complement(1539..1847)
                     /locus_tag="PLT08_16170"
                     /inference="COORDINATES: protein motif:HMM:NF037100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HNE06063.1"
                     /translation="MVKVKIPRRKLTAFCRKYRIQKLAFFGSVLREDFKPTSDLDILA
                     EFKKDHTSGLAFFAVPEELKEIFGRDVDLLTYQGIKSSRNPIRRKAILESEQVIYVSI
                     "
BASE COUNT          569 a          402 c          425 g          533 t
ORIGIN      
        1 tttctttgac gaatttgaaa atgtccagcc gttcggcttc ggtgaaggcg tagacttcgc
       61 ccgtggtgcc gccagggaca agccctgcga tcttgttggc gatcaggtat tcgatcagtt
      121 gttcaaggac ggggtagttg atgctttcgt ctgcattgaa gggggtgacg atgggggcgt
      181 atattccttt gagttgcatg aggtctccgg tttcaggtgc caagtttcag gttgaaggtt
      241 tgaaagttgg aaggttgtgt ccattataaa agaaaagacc ctgataggtc attggaatgc
      301 gagtattagc aagaaagcaa aagtgttcaa aaccgtcaac gaatggacat gaattcatga
      361 atgagagacg gctattcgag tattcgtgtc cattcgtgga tggttaattc cctcagtcga
      421 agtgggcaac aataataaat acatttaaaa accgcgaaaa gacttatctc aatagcttct
      481 cacaataagg gcaatgcaca atctttgaat gaaaaaaagg atttacacct gatttcttgt
      541 taaaaaatcc atctaaatgg ttattgcatg atggacatcg ccaattccat tgaaaataaa
      601 caagatacat taaggaatag acgacaaaaa ctccaatgaa agcccaagct aaattgctat
      661 ttcctcgaat taaaaaaatc acaagaataa aaaaaagata tatcaaataa cttatgcgtt
      721 ggcgcataag tcgcaaagta aattcatctt taatttgttt gtcagttttt tcgttcatta
      781 caccacctta attaacacag tttttgatac aactcgcgga cggcaggttt gtccgcggag
      841 gaatggcaga ggaaaacgcg atgagggcga tttgtggtca tgggatatgt ccccgtaatt
      901 atatctttgg acttagccgc gtagacggac tttcccaatt tctacaatgg cgagatgatg
      961 caaaagattt tcgccaactt catgaacggc gtttaaaact tcccgattga acacgtcaat
     1021 tggctcacta tcggggactc ttactacgag aataccttga ttttctgagg gcggaaattt
     1081 tagaatattc gaaaatccca tgtcacaagt gatgagcagg cggttttcac tttgagcaaa
     1141 ggcaaaaaca agatcgtcac ttttgccttg caagcctgct tcatgtacgt tcataacgtc
     1201 aaaacccgcg tcacggagaa cgcgggttgt tgaacgaggg acgtcttcat caatgagaaa
     1261 gcgcagggtt acgaggtcgc ccatacttgt tcctcagaaa ggcgaatggc agcgtaatgt
     1321 aaggcgttgt aaacatcctt ctcagtaata ccgtattctt cggcaacatc tttgatgctc
     1381 attccagagg caacacgcgc cacaacagta tccactggca cacgagttcc tttgattaca
     1441 ggcttgccaa aacggacaga cgggtcaaca gtaattccta aagaaagatc gaacattttt
     1501 acctcttgta ggtatcatac ttccgtgatg agtagatgtc aaatcgagac ataaatcacc
     1561 tgttccgatt ctaaaatagc tttgcgtcta attgggtttc tgctggattt tattccctga
     1621 tacgtgagca agtccacatc ccgaccaaag atttccttca attcttcggg cacggcaaag
     1681 aaagccagcc ctgaggtatg gtctttttta aattccgcca aaatatctag gtcacttgtt
     1741 ggtttgaaat cctcgcgcaa aaccgagcca aagaacgcca gtttttggat gcggtattta
     1801 cggcaaaatg ccgtcaattt tctgcggggg atttttacct ttaccatgct gaaattatac
     1861 cttttcaact cagctattaa ataactgctg tttcaaccac gcaaacgcag cggactgcat
     1921 ctccaggtc
//
LOCUS       DAOQJE010000757         1928 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_49420_length_1928_cov_7.266418, whole genome shotgun sequence.
ACCESSION   DAOQJE010000757 DAOQJE010000000
VERSION     DAOQJE010000757.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1928)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1928)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1928
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49420_length_1928_cov_7.266418"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            88..1386
                     /gene="eno"
                     /locus_tag="PLT08_16175"
     CDS             88..1386
                     /gene="eno"
                     /locus_tag="PLT08_16175"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004634 - phosphopyruvate hydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HNE06064.1"
                     /translation="MDTTIESISALEVLDSRGNPTVEVEVLLMDGSWGRAAVPSGAST
                     GEHEALEMRDGDKKRYLGKGVANAVSNVNGVIAESLFGWDASDQRGIDAEMIAMDGTA
                     NKSKLGANAILGVSLAVAKAAANSFGMPLYRYLGGVYAHVLPVPMMNIMNGGAHTSWQ
                     STDMQEFMVMPFGAPTFSEGLRWGAEIYHALKSVLKEKGYSALVGDEGGYAPALKANN
                     EAIELILAAIEKAGFKAGRGEQVAIALDPAASELYDEKTKKYNLRKEGKELTGEQMVE
                     FWAKWVKDYPIVSIEDGLAQDDWDAWKSLVKTIGDKCQIVGDDLLVTNPERVRRAIKE
                     NAANALLVKVNQIGSLTETIEAVDLCQRAGWRAISSHRSGETEDATIADLAVALNAGQ
                     IKTGAPARSDRVAKYNQLLRIESELGDTAKYAGWDALKVK"
     gene            1587..>1928
                     /locus_tag="PLT08_16180"
     CDS             1587..>1928
                     /locus_tag="PLT08_16180"
                     /inference="COORDINATES: protein motif:HMM:NF024212.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HNE06065.1"
                     /translation="MKITADQAFLRETNLSLVVRLIHNQSPLSRAQLAGITGLNKSTV
                     SSLVDELIERGLVYETGSNTGGTGRPATLLEINPNAGHIIGVELGVDFVSVAVTDILG
                     NIVWRRREDANP"
BASE COUNT          506 a          451 c          515 g          456 t
ORIGIN      
        1 ggcgaaaatt aaaaatcagg ggcgacctgt cttgggatat taacaccatc aagcaaaaat
       61 cgtcgccttc atttaggagc aaattaaatg gatacaacaa ttgaaagcat ctccgcgcta
      121 gaagttcttg attcacgcgg aaaccccact gtggaagtgg aagttttatt gatggacggc
      181 tcatggggac gcgctgcggt tccctccggt gcctcaaccg gtgagcacga agcgctcgaa
      241 atgcgcgatg gcgataagaa acgctatctc ggcaaaggcg tagccaatgc tgtctcaaac
      301 gtcaatggtg tgatcgccga atccttgttc gggtgggatg catctgacca gagaggcatc
      361 gatgctgaaa tgatcgccat ggatgggact gcaaacaagt ctaaactcgg cgcgaatgca
      421 atcctcggtg tgagtttggc ggttgccaag gcagcggcaa attccttcgg aatgcctctt
      481 tatcgctacc tcggtggcgt gtatgcgcac gtcctgccgg tgcctatgat gaacatcatg
      541 aatggtggtg cacacacaag ctggcaaagc accgatatgc aagaattcat ggtgatgccg
      601 tttggtgccc cgactttctc tgaaggtttg cgctggggcg cagaaattta ccacgccctc
      661 aagtctgtgt tgaaggaaaa ggggtacagc gcccttgttg gtgatgaagg cggttacgct
      721 cctgctctca aggcgaacaa cgaagcgatt gaactcatcc tcgctgcgat tgaaaaggct
      781 ggattcaaag cgggtcgcgg tgaacaggtg gcgattgcgc ttgacccggc tgcttctgaa
      841 ctctatgatg aaaagaccaa gaaatacaac cttcgcaagg aaggcaaaga actgaccggc
      901 gaacagatgg ttgagttctg ggcgaagtgg gtcaaagatt accccatcgt ttccatcgaa
      961 gatggtcttg ctcaagatga ctgggatgcc tggaaatctc tcgtcaaaac cattggcgat
     1021 aagtgccaaa tcgtgggtga tgatttgttg gtcacgaacc ctgaacgtgt ccgccgcgct
     1081 attaaggaaa atgctgcgaa tgcactcttg gtaaaagtga accagatcgg ttctctcact
     1141 gagaccattg aagctgtgga cctctgtcag cgtgctggct ggcgtgccat ttcatctcac
     1201 cgctctggcg agaccgaaga tgcgaccatt gccgacctcg cagttgcact caatgctggg
     1261 caaatcaaga ccggcgcccc tgcccgttct gaccgtgtgg cgaagtacaa tcaacttctg
     1321 cgcatcgaat cagagctggg tgacactgcc aagtacgcag gctgggatgc tttgaaagta
     1381 aagtaagctg aaaaactccg ttgagtttgt ctcaacggag tttttttata gttgcataaa
     1441 ctcaaggcaa ggaaaaagct agaaaggggt aaacaggcac aaggtccaga aagaagcgac
     1501 cttgcgcctg tcgatgttta atttgacaat cctcttcaaa gcgctataat ttgttggttg
     1561 ccccaacata ctaatcctag gaatctatga aaatcactgc cgatcaagca tttttacgag
     1621 aaaccaatct ttcattggtc gtaaggttaa ttcataatca atctccatta tcgcgcgcac
     1681 aacttgctgg tattaccggg ctgaacaaat cgaccgtttc aagcctggtc gacgagttaa
     1741 ttgaacgcgg cttagtgtat gaaacgggca gcaatacagg tgggacggga cgcccagcta
     1801 ccttgttaga gatcaaccca aacgcgggtc atatcattgg tgtagaactc ggcgtggact
     1861 ttgtgtctgt tgctgtgaca gatattcttg gcaatatcgt ttggcgcagg cgtgaagatg
     1921 caaaccca
//
LOCUS       DAOQJE010000758         1921 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_49830_length_1921_cov_2.615220, whole genome shotgun sequence.
ACCESSION   DAOQJE010000758 DAOQJE010000000
VERSION     DAOQJE010000758.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1921)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1921)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1921
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49830_length_1921_cov_2.615220"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(104..928)
                     /locus_tag="PLT08_16185"
     CDS             complement(104..928)
                     /locus_tag="PLT08_16185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE06066.1"
                     /translation="MIGNLRERWEFTWADIWEPLGSLPKAPRDLYIQLYRTSLDYFRR
                     RPNDQELAEIVNNPEMALSAFQAIQGRQFRDEMAVVQFLERAHSLINDFGSRILVRRY
                     GEYVSKFLKKYNLRYETAEPFSLRVRLPSVYGDIYEELRQVNETNPHLSQLMTDFEIS
                     FSDFVRTRTQRDLRTSLAKASNYAEGMAGASLNVQGGTLGHLCNQLQTFPHVAVRDSV
                     KNLYQFCSDYPAIRHAGNPNNKLRDLEIKDTIIVSALFFAMSGYLNQQVSLSNAVG"
     gene            complement(925..>1921)
                     /locus_tag="PLT08_16190"
     CDS             complement(925..>1921)
                     /locus_tag="PLT08_16190"
                     /inference="COORDINATES: protein motif:HMM:NF013579.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HNE06067.1"
                     /translation="VTTGEEHKTADTYQLDTEAVCVPMISSFGHGKAGLKRVHYIKGK
                     FALANILTALIVKEPRELSTKYLALYLQTFKDQLIVPLQTGAANMSLRPEKLAGVPIK
                     YPSLKEQERIFYLLDEVESIRRLRDKTIERTRALIPALFVEIFGNPVSNPKGWEVRPF
                     GELVRNQDGRRKPVKASDRAERKGLYPYYGASGIIDYVDDFLFEEQALLIAEDGANLL
                     ARSTPIAFIANGKYWVNNHAHVVTENGLAKLEYLCEYFNLINVKEYVTGSTQPKLNQS
                     RLNNIKIPVPPIPLQKLFGERVDEVRLIEQKIDESAKEIEKLYNSMLSTAFGGTL"
BASE COUNT          533 a          413 c          374 g          601 t
ORIGIN      
        1 gaagccagaa aaaagcctga aaatgccgca gactcccaca cgtcgggtgc acgctttgtt
       61 ggcacgcttt tgactttaag actcgttttt gtcctactga gaattatccc acagcatttg
      121 ataaactcac ttgctgattc aagtatcctg acatggcaaa aaataaagca gacacaataa
      181 tcgtatcctt gatttccaaa tctcttaatt tattatttgg atttcctgca tgacgaatag
      241 cggggtaatc agaacagaat tgatataggt tttttacaga gtctcgcacg gcgacatgcg
      301 gaaaggtttg aagttggttg cataaatgtc caagtgttcc accttgaaca ttcaaagacg
      361 cccctgccat tccttctgcg tagttgcttg ctttggctaa tgatgtcctt aagtctcgtt
      421 gcgtccttgt tcttacaaaa tcggaaaatg atatttcaaa atctgtcatc agttgcgaca
      481 agtgcggatt tgtctcattg acctgccgca actcctcata aatatcacca taaacggacg
      541 gcaagcgaac acgcaacgaa aaaggttcgg cggtttcata gcgcaaattg tattttttca
      601 ggaactttga aacatattcg ccatatcttc tgactaaaat acgagagcca aaatcattta
      661 tcaaactatg agcgcgttcc aaaaattgga caaccgccat ttcatcgcgg aattgtcgtc
      721 cttgaattgc ttgaaatgct gacaatgcca tttctggatt atttacaatc tctgctaatt
      781 cttggtcatt aggtcttcgg cggaaataat caaggcttgt cctgtacagt tgaatataaa
      841 ggtcgcgtgg tgcttttggt aaacttccca aaggctccca aatatctgcc caagtaaact
      901 cccagcgttc tcttaagttg ccaatcataa agttccccca aatgctgtag aaagcatgga
      961 gttgtaaagt ttttcaattt ccttcgcgct ttcatctatt ttttgttcta ttagacgtac
     1021 ttcatcaacg cgctctccaa ataatttctg caaagggatt ggaggaacag ggattttgat
     1081 attgtttaac cttgattgat ttagtttggg ttgagtactt ccagttacat attccttaac
     1141 attaatcagg ttgaaatact cgcaaagata ctctaatttt gccaagccat tttctgtaac
     1201 cacatgagca tggttattca cccaatattt cccattagca ataaaggcta taggcgtaga
     1261 gcgggctaac aggtttgcgc catcttctgc aattaataac gcttgttctt caaataaaaa
     1321 atcatcaaca taatcaatga ttccactagc gccataatat gggtaaagac cttttcgctc
     1381 tgcacggtca gacgctttta caggcttgcg ccttccatct tggtttctta ctaattcccc
     1441 aaatggtctg acttcccatc ctttaggatt tgagacagga ttaccgaaaa tttccacgaa
     1501 aagtgctgga attaaggcgc gagtacgttc tatagtctta tcgcgtaatc ggcgtataga
     1561 ttcaacttcg tctaaaagat aaaatatacg ttcttgttct tttaaggagg gatatttgat
     1621 tggaactccc gccaattttt cagggcgtaa actcatattt gccgcgcctg tttgcaatgg
     1681 aacaatcaac tggtctttga aggtctgtaa atataaagca agatattttg tagaaagttc
     1741 gcggggttct ttcacaatca aagccgtcag gatatttgca agggcaaact ttcctttgat
     1801 gtaatgcact cgtttcaatc ctgctttgcc atgtccgaaa gatgaaatca tcggcacgca
     1861 tacggcttct gtatccagtt ggtaggtgtc ggcggtttta tgttcttcgc ctgtcgttac
     1921 c
//
LOCUS       DAOQJE010000759         1914 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_50117_length_1914_cov_7.681011, whole genome shotgun sequence.
ACCESSION   DAOQJE010000759 DAOQJE010000000
VERSION     DAOQJE010000759.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1914)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1914)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1914
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50117_length_1914_cov_7.681011"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..899)
                     /locus_tag="PLT08_16195"
     CDS             complement(<1..899)
                     /locus_tag="PLT08_16195"
                     /inference="COORDINATES: protein motif:HMM:NF013225.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxyl transferase domain-containing protein"
                     /protein_id="HNE06068.1"
                     /translation="METLSSALDPQSPEFKANAEHNRALASELKKRLALVREGGGEKY
                     RKRHEEQGKLFVRERIERLLDPGAPFLELSALAAWDMYKEKGSDSLTAPSAGIVTGIG
                     RISNREVMIVANDATVKGGSYFSMTVKKHLRAQQVAEENHLPCLYLVDSGGAYLPLQD
                     EVFPDAHHFGRIFYNQARMSAKRIPQIAAVMGSCTAGGAYVPAMSDEAIIVKGAGTIF
                     LGGPPLVKAATGEDVTAEELGGADVHTRKSGVADHFAEDDDHAIEILRGIVETLQRSN
                     VNTFLSALTVAPPEEPLYNPDEL"
     gene            complement(1232..>1914)
                     /locus_tag="PLT08_16200"
     CDS             complement(1232..>1914)
                     /locus_tag="PLT08_16200"
                     /inference="COORDINATES: protein motif:HMM:NF024816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNE06069.1"
                     /translation="KMLSSALSFCESRYFVSVEGDIWSLYAQYFLLMLREGAIIDILN
                     SNNEYRISAIKVAQRALELADEWQKIRGRSNPNPRDYTRGLWLLGAAYRANNELTLAE
                     ENLSKALNLCRQINLVDHEANILLDLARLRYAQGDFKDAQEKASEALVITERCGYVLQ
                     GADVNLFLATLALDGYKLQVTSSMSDKEAARYHAQEALKLATCDGPPYYYKVAYEEAE
                     QLLARLKE"
BASE COUNT          443 a          492 c          493 g          486 t
ORIGIN      
        1 tacaattcat cagggttgta taaaggttct tcggggggag caactgtcag cgcggaaaga
       61 aacgtgttaa cgtttgaacg ttgtaacgtt tcaacgattc cccgcaggat ctcaatcgca
      121 tggtcatcat cttccgcaaa atgatcggca actccagatt ttctcgtgtg gacatctgcg
      181 ccgcccagtt cttcggcagt gacatcctcg cccgtggcgg ctttgacgag aggcgggccg
      241 cccaaaaaaa tggtacccgc acctttgaca atgatggcct catcgctcat cgcagggacg
      301 tacgcgccgc ccgccgtgca gctgcccatg acagcggcga tctgcggaat gcgcttggca
      361 gacatgcgcg cctgattgta gaaaatgcgt ccgaagtgat gcgcgtcggg gaagacctca
      421 tcttgcaatg gcaaatacgc gccgcccgaa tcgacgagat acaaacaggg cagatgattt
      481 tcctcggcga cctgctgcgc gcgcaaatgc tttttgacgg tcatcgaaaa atacgaaccg
      541 cctttgacgg tggcgtcgtt ggcaacgatc atcacttcgc ggttggagat tcgtccgatg
      601 ccagtgacaa tgcccgcgct gggagcggtg agagagtcgc ttcccttttc tttatacata
      661 tcccaagccg caagcgcaga gagttcaaga aatggtgcac cagggtcgag gagacgttcg
      721 atgcgttcgc gcacgaagag cttaccctgt tcttcatgtc gcttacggta tttctcgccg
      781 ccaccttcac ggacgagggc gagccgcttt tttaattcac tcgcgagggc gcggttgtgt
      841 tctgcattgg ctttgaattc gggggattgc gggtcgagcg cggaggagag agtttccatt
      901 tttagtttgg tagtcccgtg gttggatggt tgtcagtaaa cagttgtcag ttgacaataa
      961 tcagtgtgag gcaagtctag cataaaaaat gggaatgagt aacgaataaa aaagactcgg
     1021 cgatgggctg agtcctttgg ttatgcttga tttatgacag tggtttcgac ccccacccgt
     1081 cctcccccaa atacgacatt gaagcgcttt ctagtcagct aaaatttata tgtcgtattt
     1141 gggggaggtg ccccgcgaat gcgggcggag ggggtgggtt aaagtttttc caacattctc
     1201 tccgcctctt cacccgtagg ggacaacctt gtcactcttt caagcgagca agtaattgct
     1261 ccgcttcctc atacgccacc ttgtaataat agggcggacc gtcgcacgtc gccaacttca
     1321 acgcctcttg tgcatgatat ctcgctgcct ctttatcgct catggaactt gtaacctgca
     1381 acttgtaacc atccaacgcg agcgtagcga ggaacaaatt cacatccgcc ccctgcaaca
     1441 cgtacccaca tctctccgtg atcaccagcg cctcggacgc tttttcctgc gcgtccttga
     1501 aatcgccttg agcgtagcgt aaccgcgcta ggtcgagcag gatgttggct tcatggtcaa
     1561 ccaaattgat ttgccgacaa agattgagcg ctttggacag gttttcttct gccagagtca
     1621 attcgttatt ggcgcgatag gctgcgccga gaagccaaag accacgagtg taatctctgg
     1681 gatttggatt ggaacgcccc cttatttttt gccattcatc tgccagttca agggcgcgtt
     1741 gggcaacttt gatagccgat attcgatatt cgttatttga gtttagaata tcaattattg
     1801 ccccttccct tagcatgagt aggaaatact gagcatagag actccaaata tctccttcga
     1861 cactaacaaa atatctactt tcgcaaaatg acaaagcaga ggatagcatc tttt
//
LOCUS       DAOQJE010000760         1911 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_50273_length_1911_cov_10.574892, whole genome shotgun
            sequence.
ACCESSION   DAOQJE010000760 DAOQJE010000000
VERSION     DAOQJE010000760.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1911)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1911)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1911
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50273_length_1911_cov_10.574892"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            95..1750
                     /locus_tag="PLT08_16205"
     CDS             95..1750
                     /locus_tag="PLT08_16205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE06070.1"
                     /translation="MKSYSKIIFYLTVSVLLISCGQPLALPTATPISILASPNTPKLT
                     PTQEIAATATPPPYYSNILPTINPTELPSLLESAFSVETLDTLNGHRLRRVSGWDYGF
                     NYTDEHVAPYFAYRYQWMDSHHLLLFPKIGEVQHPNWITEQKRAVVFNLDTGTTWLPP
                     ADEPREDNRVLNISLPYWSPTLGILITSQTMGGGYTTSQGVSIYNANGDFIDRYWGDL
                     IGISPSGTKILIADDSWVDLETGRIVDFNWYFGDHPRWRPIWSQDETQIYMCCYFYGN
                     ATTGESYYLLNKDTIFEGKPIDFGKSLHHSHGVWLNDNYVLAQADGFYTFMSYFEGMI
                     PVFDIQAKTFRDLIKLANLPYDSSDLDTQYAQKDISPKGDYMWIHKIYYPTGFLIDLK
                     TFRSWEYAGNLSWVRNGEYAVVDSKLLVLSSKELKAMPTDIQCHNWHPTKGVCLLLTT
                     NEPDTSTLNFLNVQDMSVQKTALSSPLFNQAIWSSNGDYIILTTEDKTLWRIDYPSLE
                     NMEQLTRPVADMYIESIIWSPDNEYLSFVGGTNIYIVDINKSQ"
BASE COUNT          578 a          402 c          371 g          560 t
ORIGIN      
        1 tggttggacg gcttcgccgt ccccacccca gcgcaggtaa cgcaaaccgt tgggcagaac
       61 cttgtgtaat tcatttaggg aaaggactct gataatgaaa agttattcaa aaattatttt
      121 ttatctgaca gtatcagtac tattgatttc ttgtggtcaa ccacttgcac tccctacagc
      181 aacaccaata tccatacttg catcccccaa tactccgaaa ttaactccaa cacaagaaat
      241 tgccgcaaca gcaacgcctc ccccatatta ttcaaatata ctaccaacaa ttaaccctac
      301 agaacttcct agcctgcttg aatccgcgtt ttctgttgaa actttagaca cattaaatgg
      361 acatagattg cgacgtgttt cgggctggga ttatgggttt aattacactg atgagcacgt
      421 agcaccttat ttcgcctacc gctatcaatg gatggattcg catcacttgt tattgtttcc
      481 aaaaataggt gaagtacagc atcctaattg gataacagaa caaaagcgag ccgttgtttt
      541 caatctcgat actggaacta cttggttacc gcccgcagac gagccaagag aagacaaccg
      601 agttttgaat atttcgttac cttattggtc gcccacgctt ggcatcttga ttacatcaca
      661 aacaatgggt ggtggttaca caacttcaca gggagtttct atttataatg caaatggcga
      721 ttttattgat cgttactggg gtgatttaat tggcatatct ccaagcggaa caaaaatcct
      781 tatcgctgac gatagttggg tggatttaga aactggaaga attgttgact tcaattggta
      841 ttttggcgac catccaagat ggcgcccgat ttggtcacaa gatgaaacgc aaatatatat
      901 gtgctgctat ttttatggaa acgcgacaac tggcgagagt tattatcttc tcaataaaga
      961 tacaattttt gaggggaagc ctatagattt tggaaaatcc ctacatcact cacatggcgt
     1021 atggctaaac gacaactatg tattagcaca agcagatgga ttttatacat tcatgtcata
     1081 ttttgaaggg atgattcctg tatttgacat tcaagcgaaa acatttcgcg atttgataaa
     1141 actagcaaat ctgccatatg actcctcaga ccttgataca caatatgccc aaaaagatat
     1201 ttctcctaaa ggagattaca tgtggataca caaaatatat tatccaactg gttttttgat
     1261 tgaccttaag acctttcgtt cttgggagta cgctggaaat ctgtcttggg taagaaatgg
     1321 tgaatatgca gttgttgact caaaactact tgtactttcc agtaaagaat taaaggcaat
     1381 gcctacagac atacaatgtc ataattggca tccgacaaaa ggcgtatgtt tattattaac
     1441 cacaaacgaa ccagatactt caacgctaaa ttttctaaat gtacaagata tgtctgttca
     1501 gaaaaccgca ttgtcaagcc cacttttcaa tcaagcaatc tggagttcca acggcgatta
     1561 tattattttg acaacggaag ataagacttt atggcggatt gattatccaa gtcttgaaaa
     1621 catggaacaa ttaacacgcc cagttgcaga catgtatatt gaaagtataa tttggtcgcc
     1681 cgacaatgag tatttatcct ttgttggcgg tacaaatatt tatatagtgg acatcaacaa
     1741 aagccagtaa aatgtcgaca agatggcaat gtcttctttt tcaattagcg agtcttgcgc
     1801 tcatcggttc tgcccaacaa agcgtgcacc tgacgctggg gattctgcgg caatctcaag
     1861 cagtttccta cgccttagcc tttttctggt tggacggctt cgccgtcccc g
//
LOCUS       DAOQJE010000761         1911 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_50283_length_1911_cov_4.480603, whole genome shotgun sequence.
ACCESSION   DAOQJE010000761 DAOQJE010000000
VERSION     DAOQJE010000761.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1911)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1911)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1911
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50283_length_1911_cov_4.480603"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..312)
                     /locus_tag="PLT08_16210"
     CDS             complement(<1..312)
                     /locus_tag="PLT08_16210"
                     /EC_number="5.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012521.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate mutase family protein"
                     /protein_id="HNE06071.1"
                     /translation="MQLYFIRHGQSINNANSENEGYQEHSDPYLTEIGKKQAQITAQY
                     LKERQTLSDPESWNSQNQFGFGFTHIYCSLMERATETASYTARAIGNIRFEAWVDIYE
                     EG"
     gene            complement(387..716)
                     /locus_tag="PLT08_16215"
     CDS             complement(387..716)
                     /locus_tag="PLT08_16215"
                     /inference="COORDINATES: protein motif:HMM:NF016268.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BrnT family toxin"
                     /protein_id="HNE06072.1"
                     /translation="MKKQKALVFNWEAAKAARNEEKYGVSFEEAKVIFTDKYAVYHDD
                     VEHSQKEKREIVIGRNFNTKRHLTCFFTRVDGVIHIIGARLSTHREREEYKENSDEAK
                     RIIYQIK"
     gene            complement(754..1539)
                     /locus_tag="PLT08_16220"
     CDS             complement(754..1539)
                     /locus_tag="PLT08_16220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE06073.1"
                     /translation="MSLGKKIAIGFGVAFILLCIAAIAFVNWSNSDAGQATFADVGTQ
                     QAVASATGAVQTQQASGTATAVWAEIDARLQNATLIYENTFDEGTPLVNKFGEDLKPY
                     YRDGIPEVIVAWNGSTTLPIGQSVSDFVAEVDCMANGAFCGIAYDINPGKDDFDVYFA
                     SGIGTADRFFFSDLYASLPSTNWGGENFPASPAEGFLHRIRVERFGQVMRFYVNGQLM
                     EERLLDAPGSASGDVGFYFGKASEESGGIQTVQFDNFRVWELP"
     gene            complement(1684..>1911)
                     /locus_tag="PLT08_16225"
     CDS             complement(1684..>1911)
                     /locus_tag="PLT08_16225"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: protein motif:HMM:NF012912.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthetase"
                     /protein_id="HNE06074.1"
                     /translation="DGKSVTYSEVDAYGLDKVECVYESFPGWSEDISKAKSFEELPLN
                     AQKYVKMIEDAAGVPVKWIGVGPARDETIRR"
BASE COUNT          454 a          449 c          471 g          537 t
ORIGIN      
        1 gccttcttcg taaatatcca cccaggcttc gaagcggatg ttgccgattg cgcgtgcggt
       61 ataggaggca gtttcggtcg cgcgttccat caggctgcaa tagatgtggg tgaagccaaa
      121 gccaaattga ttctggctat tccatgattc ggggtcgctg agcgtttggc gctctttgag
      181 atattgtgct gtgatctggg cttgtttttt gccgatctca gtgagatacg ggtcggaatg
      241 ttcttgatag ccttcattct ctgaattggc gttgttgatc gattgtccgt gacggatgaa
      301 gtagagctgc atggttttcc tttcgagaaa aaacaacagt catttcagcg aaatgactgt
      361 tgtttgaaat ttatgttgac ctgattttac ttgatttggt aaatgattct tttggcttcg
      421 tcggagtttt ctttgtactc ttctcgctct cgatgcgtgg acaatcttgc gccgatgatg
      481 tgaatgactc catctacacg ggtgaagaag caggtcaggt gacgtttggt attaaagttg
      541 cgtccaatca caatttcgcg cttctctttt tgggaatgtt ctacatcatc gtgatagacc
      601 gcgtatttat ctgtaaaaat gactttggct tcttcgaagc tgacaccata tttttcttcg
      661 ttacgggcgg ctttggcagc ttcccagttg aatacaaggg ctttttgttt tttcatgtac
      721 tcgagtatag catgacagta ggggtgggta aatttacggc aattcccaaa cgcggaagtt
      781 atcgaactgc acggtttgaa tgccaccgct ttcttcagat gccttcccga agtaaaagcc
      841 cacgtcacca gaggcagacc caggcgcgtc gaggaggcgt tcttccatca attgcccgtt
      901 gacatagaaa cgcatgacct gcccaaagcg ttctacgcgg atgcggtgta aaaatccttc
      961 ggcaggagaa gcggggaagt tttctccgcc ccaattggtg gagggcaggg aggcgtacag
     1021 atcgctgaag aagaagcgat cggcggtgcc aatgcccgat gcgaaataga catcgaagtc
     1081 atctttgccg gggttgatat cgtatgcgat accgcagaaa gccccatttg ccatgcagtc
     1141 cacttcggcg acaaaatcgg aaacggactg accgatgggc aaagtcgtgg acccgttcca
     1201 ggctacgatg acctcaggga ttccatcgcg ataatagggt ttaaggtctt caccaaactt
     1261 gttgacaagt ggcgtgcctt catcgaaggt attttcgtaa ataagtgtgg cgttctgtaa
     1321 acgggcatcg atctctgccc aaactgctgt ggcagtgcca gaagcctgct gggtttgcac
     1381 cgcgcctgtg gcgctggcaa ccgcttgctg tgtgccaaca tcggcaaaag ttgcttgccc
     1441 ggcatctgaa tttgaccagt tgacaaaggc aattgcagcg atacatagta agataaaggc
     1501 aactccgaac ccgatggcga tcttctttcc aagtgacata aagatattct cctaaaatgt
     1561 aatatcaaca attatattga atctgccgat tttgtttgcg gtacaaatct cctataaaaa
     1621 atataggtga cagccactta caaagtgact gtcacctgtt tcttatttgc tgaccaccaa
     1681 tttctatctg cgaatcgtct catcccttgc cggaccgacc ccgatccact tgacaggcac
     1741 acctgccgca tcttcgatca tctttacata cttctgtgcg ttgagaggta actcttcaaa
     1801 ggactttgcc ttgctaatgt cttcgctcca accggggaag gattcataga cacattcgac
     1861 cttgtccaaa ccgtaggcat ccacttcaga gtatgtgacc gacttcccat c
//
LOCUS       DAOQJE010000762         1908 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_50442_length_1908_cov_6.647059, whole genome shotgun sequence.
ACCESSION   DAOQJE010000762 DAOQJE010000000
VERSION     DAOQJE010000762.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1908)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1908)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1908
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50442_length_1908_cov_6.647059"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..466
                     /locus_tag="PLT08_16230"
     CDS             <1..466
                     /locus_tag="PLT08_16230"
                     /inference="COORDINATES: protein motif:HMM:NF040134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE06075.1"
                     /translation="VGAVLLAAVCIAEYISVDPTAPLYGFARAGLTAVAYALFLILVT
                     SLRFSGARMVLVIPSVLIVAGLISLRILHLDGTDRWDFPWAVGIGLVCAQIGAGLHYW
                     PLSPVQFGLAVTGPLYALTMLSNSLTENIPLRRAVVGPIIVAGVAWVTAIFL"
     gene            469..885
                     /locus_tag="PLT08_16235"
     CDS             469..885
                     /locus_tag="PLT08_16235"
                     /inference="COORDINATES: protein motif:HMM:NF025819.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M67 family metallopeptidase"
                     /protein_id="HNE06076.1"
                     /translation="MRTLSLTNEQLQQMIAHVDSQAPLEACGLLAGRGSSVESVLTVT
                     NQAQSPVRYVMDPIEQLHAFEWIESQGLELLGIFHSHPAGPEVVSPTDIAEAAYDVVY
                     VIVSRINSPWKARGFWIQDGKFDEVTLQIASSSNQP"
     gene            955..1641
                     /locus_tag="PLT08_16240"
     CDS             955..1641
                     /locus_tag="PLT08_16240"
                     /EC_number="2.3.1.275"
                     /inference="COORDINATES: protein motif:HMM:NF014690.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043772 - acyl-phosphate
                     glycerol-3-phosphate acyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate acyltransferase"
                     /protein_id="HNE06077.1"
                     /translation="MQIVLAILAVVLAYVIGSVPVGLLIVKIVTGKDIREVESGRTGG
                     TNAMRAAGFWAGFATAMLDIVKGAAGVWVARWLTPDLPVVHMIAPLAAILGHNHSIFL
                     PERDENGRIIRLRGGAGGAPSVGGAMGLYLPSVLIVLPLGMLTFFSIGIASVTTMAVA
                     LYSTLAFAYYASQGIIPWAYVLYGVGAEILLVLALRPNIKRLFEGNERVVKYSLNGWL
                     RKRKEEKAAK"
BASE COUNT          399 a          462 c          518 g          529 t
ORIGIN      
        1 ggttggtgca gtgttgctgg cagccgtttg tattgccgaa tatatttccg tcgacccaac
       61 cgctcctttg tatgggtttg cgcgtgccgg tcttactgct gttgcctatg ccttgttctt
      121 aatccttgtc acttcgttaa gattttccgg tgcgcgcatg gtgctggtga ttccatcagt
      181 gctcatcgtt gccgggttga tcagtttacg cattctgcac ctcgatggca cagaccgttg
      241 ggattttcca tgggcggtag gaattggttt ggtctgcgcg cagattggcg ccgggttgca
      301 ctattggccg ctttcgccgg ttcaattcgg gctggctgtg accggtccgc tatatgcgtt
      361 gaccatgctc tcgaacagcc ttacagaaaa tatcccattg cgccgcgcgg tagtgggacc
      421 catcatcgtg gcaggcgttg cttgggtcac tgcgatcttt ttgtaagcat gcgcactctt
      481 tcccttacca acgaacaact tcaacagatg atcgctcatg tcgattcgca agctccactg
      541 gaagcttgtg gtctattagc gggtcgcggc tccagcgtgg agtctgtgtt gacggtgacg
      601 aatcaggcac aaagtccggt gcgatacgtg atggatccca ttgagcaatt acatgccttc
      661 gagtggatcg agtctcaagg gctagaactg ctcggcatct tccattcaca cccagccggc
      721 cccgaagtgg tttctcctac tgatattgct gaggctgctt atgatgtggt gtatgtaatc
      781 gtttcccgca ttaacagccc atggaaagcg cgcggatttt ggattcaaga cggcaagttt
      841 gatgaagtga ccttgcagat tgcctcatct agcaaccaac cctaaggttt cgtgttttta
      901 cgtctgctaa tttgtgatgt taaattttaa acagcaactc gtggaggagt cttaatgcaa
      961 atcgtcttag ccattctggc agttgtactg gcttatgtaa tcgggtcggt tccggtgggg
     1021 ttactgatcg taaagatcgt gaccggaaaa gatattcgcg aagtggaaag cgggcgcacc
     1081 ggcggtacga acgccatgcg tgcggcaggg ttttgggctg ggttcgctac tgccatgctc
     1141 gacattgtga aaggcgcagc aggggtttgg gtggcacgtt ggctcacccc cgatctgccc
     1201 gttgttcaca tgatcgctcc cttggcggca attctcggac ataaccattc gatctttctg
     1261 cccgaacgcg atgagaacgg acggatcatt cgtttacgtg gcggtgcagg cggcgctcct
     1321 tctgtaggcg gcgcaatggg tctgtatctt ccatctgttt tgatcgtcct gccgcttggc
     1381 atgttgacct tctttagtat tgggatcgct tcggtcacaa ccatggcggt ggcgttgtat
     1441 tccactttgg catttgccta ttatgcgtca cagggcatca tcccttgggc gtatgttctt
     1501 tatggggtgg gggcagagat tctgttggtg ttggcattac gcccgaatat caaacgtctc
     1561 tttgagggca atgaacgggt tgtaaaatac agcctcaacg gctggctgag aaaacgtaaa
     1621 gaagaaaaag cagcgaagta agctaaaacg ccgaggtctt gaagacctcg gcgttttttt
     1681 acccttgaca gattagagta tttactctaa aatactagag taaatactct aggagacatt
     1741 atgtccactc gtgaaaagat tttagatacc gccctcaccc tcttcaataa cgaagggact
     1801 gccgcggtca gcaccaacca catcgccgag gcggcaagca tcagtccggg gaatctctac
     1861 tatcacttcc acaataagga agagattatt cgaggtctat tcgagcgt
//
LOCUS       DAOQJE010000763         1900 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_50847_length_1900_cov_4.431436, whole genome shotgun sequence.
ACCESSION   DAOQJE010000763 DAOQJE010000000
VERSION     DAOQJE010000763.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1900)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1900)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1900
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50847_length_1900_cov_4.431436"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..655)
                     /locus_tag="PLT08_16245"
     CDS             complement(<1..655)
                     /locus_tag="PLT08_16245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE06078.1"
                     /translation="MQSQTTSETSQQKATERVYRTWREGFIISLLVGVLIFGAVALLP
                     ALNASESIVIDGIFISTYIIVALATIFKFPYNIRVTAFLLSVYVLGIGELISLGILGD
                     GLFYFIALVIFATMLVSPRAGSVSIAISIITFIVIGWLMISGRFAPLFPVVAPAKLAD
                     WFSASAALIMFGAVIIIGFGQLEKEFAGAQAKITSTLDELREERNNLDNRVQERTNQL
                     "
     gene            complement(655..>1900)
                     /locus_tag="PLT08_16250"
     CDS             complement(655..>1900)
                     /locus_tag="PLT08_16250"
                     /inference="COORDINATES: protein motif:HMM:NF024883.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HNE06079.1"
                     /translation="FFTTIASQVAGVIKNVQLLDESKQKALQVETAAEIARDISGSLN
                     LDELLSKAVNLIRDRFNFYHAGIFLLDIPGEFAIIREATGEAGAQMKRQGYKIGVGSK
                     SIVGYVTGKGETLVVSDTTKDITYYPNPLLPETRAEAAFPLRVGERILGALDVQSIQP
                     YGFHEDNLRSLQILADQLAIAVANTELFAETQEHLSQHRLLHHITSSAASGTTLEEAL
                     ESAVSGLQVTLGGDRVIILLVDREKRILEVKASIGYSEDISSMTVEVGKGVTGWVAAH
                     RRPLRIRDVREDSRYIETSPNTRSEMAIPLIYRNEILGVINVESELVDAYSENDEEML
                     GTLGGSLAAVIANARLVEQIRAQSERERLINEITARIRRSTDIQSILHTTASEISRVT
                     GARYTKIQVEPKSADTPSDERQ"
BASE COUNT          504 a          457 c          439 g          500 t
ORIGIN      
        1 gaagctgatt tgtacgttct tgcactctgt tatcgagatt atttcgttcc tcgcgtaatt
       61 catcgagtgt agaagtgatt tttgcttgtg cacctgcaaa ctctttttcc aactgcccga
      121 aaccaatgat gataactgct ccgaacataa tcagcgcagc gctggcgctg aaccagtcgg
      181 caagtttggc aggagccaca acagggaaga gcggggcaaa acgcccgctg atcatgagcc
      241 aaccgatcac gataaaagta ataatactaa ttgcgattga gacactgccg gctctaggcg
      301 ataccagcat tgtggcaaaa atgaccagcg caatgaaata gaaaagaccg tctcctaaga
      361 tcccgaggct gatgagttcc ccaatgccta acacatacac gctcagtaag aatgcggtta
      421 cgcggatgtt gtaaggaaac ttgaaaatcg tagcaagagc cacgatgata taagttgata
      481 taaagatgcc atcgataaca atgctttcag aagcattgag cgcaggtaag agtgctacag
      541 cgccaaatat cagtacaccg accaaaagag aaataataaa accttcgcgc caggttctat
      601 aaactctttc agttgctttt tgttgagagg tttctgaagt ggtttgagat tgcattattg
      661 cctctcatca ctaggtgtgt cggcgctttt cggctccacc tgaatttttg tataacgcgc
      721 acccgtaacc cttgatatct cactggcagt tgtgtgtaga atggattgaa tatctgtgga
      781 tctgcggatg cgggcggtga tctcattgat aagacgttcg cgctcagact gcgcccgaat
      841 ttgctccacc aaccgggcat tagcgatcac tgctgccaaa ctgccgccga gcgttccaag
      901 catttcctca tcgttctcag aatatgcatc aactaattca ctttccacgt tgatcacacc
      961 cagaatttca ttgcggtaaa ttaaagggat tgccatttca gagcgggtat tgggactcgt
     1021 ctcaatatag cgcgagtctt cgcgtacatc gcgaatccgc aaggggcgtc tgtgagccgc
     1081 cacccagccg gtgacacctt tgccaacctc aaccgtcatg ctggaaatat cttccgaata
     1141 cccaatcgaa gctttcactt ccaatatacg tttttcgcga tcgaccaata aaataatcac
     1201 ccgatcaccg cccagtgtta cttgaagtcc actgacagcg ctttccagtg cttcttctag
     1261 tgtagtacct gaagcggcag aggatgtgat gtgatgcaat agacgatgct gtgagagatg
     1321 ttcttgggtt tctgcaaaca attctgtgtt tgcaaccgcg attgcaagtt gatctgcaag
     1381 aatctgtaaa cttcgcagat tatcttcatg aaaaccatag ggctgaatgc tttgaacatc
     1441 taatgcgccc aaaatacgtt cgccaactcg caaggggaaa gcggcttcgg cgcgggtttc
     1501 tggaagaagt gggttggggt agtaggtgat atcttttgta gtatcactga ctaccagcgt
     1561 ttcgcctttt ccagtgacgt accccacaat cgattttgag ccgacgccga tcttatatcc
     1621 ttggcgcttc atttgagcac ccgcttcgcc agttgcctcg cgaatgatgg caaattcacc
     1681 cggaatatct agcaagaaaa ttccggcatg atagaaatta aaccgatcgc ggattaagtt
     1741 gacagccttg ctcaaaagtt cgtcaaggtt caatgagcca ctaatgtcgc gggcgatttc
     1801 agctgcagtt tctacttgca aggctttttg cttgctctca tcgagaagct gaacattctt
     1861 gatgactcca gctacctgac ttgcaatggt agtgaagaac
//
LOCUS       DAOQJE010000764         1899 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_50943_length_1899_cov_2.279826, whole genome shotgun sequence.
ACCESSION   DAOQJE010000764 DAOQJE010000000
VERSION     DAOQJE010000764.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1899)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1899)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1899
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50943_length_1899_cov_2.279826"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..283
                     /locus_tag="PLT08_16255"
     CDS             <1..283
                     /locus_tag="PLT08_16255"
                     /inference="COORDINATES: protein motif:HMM:NF024579.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE06080.1"
                     /translation="FEKRGMLYLDELDDPELAIEDFSKAIRIQPSAEVYIYRCWAHME
                     NKDPDAAVKDYKRAKALDINFVKNSQRTRSLFKLRQWEQFMEEADKLLF"
     gene            296..1612
                     /locus_tag="PLT08_16260"
     CDS             296..1612
                     /locus_tag="PLT08_16260"
                     /inference="COORDINATES: protein motif:HMM:NF015722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HNE06081.1"
                     /translation="MEKLKVFLCHASEDKPVVRHFHQKLQMEGWIDAWLDEEKILPGD
                     DWHLRITEAVQDSDIVIVFLSSKSTNKRGYVQKELKFALDVALEEPEGAVFIVPIRLD
                     NCTPPRSLTKWQYTDYFPIGQQDFAYSKVLENLENRAISLGLRNANHKKSKGLSPAIR
                     TWVGAEFVLIPKGSFLMGTDKGYSFEQPLHEVNISYDYWMSRFPVTVEQYRKFIDSTD
                     RWKYLWNKDYWDEWEKRKNHPAVNVAWDEAMSYCQWLNNNKNEVLPSGLMIRLPTEAE
                     WEKACRGVDGREWAWGNTFDINKCNCKGNDIGTTTPVGSYSPDGDSPYGCADMIGNAM
                     EWVRTQSTSYKYYDINKHEDVTDDLNNSKRIARGGSFDEKAHTCYARSDFDFGITRNE
                     IGFRIVVAPSLGGESYLTSRTFAFWKDMQDKWRMPLNGTLQSLRKK"
BASE COUNT          626 a          343 c          428 g          502 t
ORIGIN      
        1 ttttgaaaaa agaggaatgc tttatctgga cgaattagat gacccagaat tggcgattga
       61 agatttttca aaagcgatta ggatacaacc aagtgccgaa gtgtacattt ataggtgttg
      121 ggcgcatatg gagaataaag acccagacgc ggcagtaaaa gattataaac gtgccaaagc
      181 ccttgatatt aattttgtaa aaaatagcca acgaacaaga agtttattca aacttcgtca
      241 gtgggaacaa tttatggaag aagcagacaa gttattattt tgagagcgtg tgacgatgga
      301 aaaattaaaa gtctttctct gccatgcatc cgaagataaa ccagttgtcc gacactttca
      361 tcaaaaattg cagatggaag gatggattga cgcttggtta gatgaagaaa aaatactccc
      421 tggtgacgat tggcacttaa gaattactga ggctgttcaa gattcagata tagtaattgt
      481 ttttctttct agcaaatcaa caaataaacg cgggtacgtt caaaaagaat taaaatttgc
      541 cctagatgtt gcgctggaag aacctgaagg tgcagtgttt attgttccaa ttcgattgga
      601 taactgtacc cctcccagaa gtctaacaaa atggcaatat acggattatt ttcctattgg
      661 gcaacaagat tttgcataca gcaaagttct agaaaaccta gaaaacaggg ctatatcgct
      721 tgggctacga aatgctaatc ataaaaaatc aaaaggatta agtcctgcaa tacgcacttg
      781 ggtaggtgca gaatttgttt taataccaaa aggcagtttc ttaatgggga ctgataaagg
      841 ttacagtttc gagcaaccat tacatgaagt taatatttca tacgattact ggatgtctcg
      901 ttttccagtt acagttgagc agtatagaaa gtttattgat tcaactgacc gatggaaata
      961 tttatggaac aaagattatt gggacgaatg ggaaaaaaga aaaaatcacc ccgccgtgaa
     1021 tgttgcatgg gatgaagcga tgtcctactg tcaatggcta aacaacaaca aaaatgaagt
     1081 tcttccatca ggattaatga tacgccttcc caccgaagcg gaatgggaaa aggcatgtcg
     1141 tggtgttgat gggcgagaat gggcttgggg aaatactttt gacatcaata aatgtaattg
     1201 caagggcaat gatattggta caacaacgcc agtaggttct tattcacctg acggcgactc
     1261 cccgtatggt tgtgcggaca tgattggcaa tgcaatggaa tgggtaagaa ctcaatccac
     1321 aagttataaa tattacgata taaataagca cgaagatgta actgacgatt tgaacaatag
     1381 caaacgaatt gcgcgtggag ggtcatttga tgagaaggca cacacatgtt atgcgcgttc
     1441 agattttgac ttcggcataa caagaaatga aataggattt agaatagtag ttgcgccaag
     1501 tttaggagga gaatcctatc tcacttcaag aacgtttgca ttctggaaag atatgcagga
     1561 taaatggaga atgccgctca acgggacttt acaaagttta cgaaaaaagt aagcccgaaa
     1621 tcaaacgccg ccgaacaaag cgtgcacccg acgtgtggga gtctgcgcga tttacaagca
     1681 tttttctggc ttcgggcttt ttctacacct caaaccttgt ccacgcccgc ccacccgcgg
     1741 gtaacgcaag ccgttagcct gctcgcgcaa aatgtaggtt tatgtcaaat cccctaattg
     1801 ttgctcatcg tggtgcttct caacttgcgc cagaaaatac tttaaaagcc tttgagtatg
     1861 caattcaaaa aggtgcagac acgattgagt ttgacgttc
//
LOCUS       DAOQJE010000765         1896 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_51084_length_1896_cov_2.662140, whole genome shotgun sequence.
ACCESSION   DAOQJE010000765 DAOQJE010000000
VERSION     DAOQJE010000765.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1896)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1896)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1896
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_51084_length_1896_cov_2.662140"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..312)
                     /locus_tag="PLT08_16265"
     CDS             complement(<1..312)
                     /locus_tag="PLT08_16265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE06082.1"
                     /translation="MKSLYVKQFVDEGIGNSSYLIASEETGIAAVIDPQRDVDKYVRT
                     AEGLGLKLRYALDTHLHADFVSGAHELAHHLGIAHDHHHFEIGASASGHADFEHISLH
                     EG"
     gene            complement(326..1168)
                     /locus_tag="PLT08_16270"
     CDS             complement(326..1168)
                     /locus_tag="PLT08_16270"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase"
                     /protein_id="HNE06083.1"
                     /translation="MSTYTHPEVLVETDWLAQHLHDPKVRIIESDEDFLLYDTGHIPG
                     AVKITWHEDLNHPIVRDYLDSIQFASLLSEKGIDNDTTVVFYGDKNNWWATYNFWVFK
                     LFGHKDVRILNGGRKKWEDEKREFTKEVPSYTKTVYKAPKRDDKKIRVFREDVLKHVK
                     NNLPLVDVRSPDEFSGKKLHMPEYPQEGALRGGHIPGAKNIPWAKAVREDGTFKSAEE
                     LKELYESVGITADKDIVTYCRIGERSSHSWFVLTYLLGYPNVRNYDGSWTEWGNLVNV
                     PIEK"
     gene            1461..>1896
                     /locus_tag="PLT08_16275"
     CDS             1461..>1896
                     /locus_tag="PLT08_16275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE06084.1"
                     /translation="MKNKYLKFLFPIALILALTLSMIGTRSVLADDAPPTPTDEVPTE
                     TPPVEEPSETQEPLEQTVPEILEQLPAETEIVVLDENSEPLPLVSNEAANVLVTGDPQ
                     WCPAGVTPGTDTLSQCTVAHASFNDLINDLQTNSATYYGSGTI"
BASE COUNT          480 a          446 c          427 g          543 t
ORIGIN      
        1 gccttcatgg agagagatgt gctcaaagtc tgcgtgtccg ctggcgctgg caccgatctc
       61 aaagtgatga tgatcgtgcg ctatgccgag gtgatgtgcc agctcatgtg ccccagagac
      121 aaagtctgca tgcaggtggg tgtcgagcgc ataccgcaat ttcaagccga gaccttccgc
      181 ggttctcacg tatttatcca catcacgttg tgggtcgatc acggcggcaa tccccgtctc
      241 ttcagaggcg atcaaatacg acgaattgcc aatcccttca tccacgaact gctttacata
      301 caatgatttc attctttatc cttggttatt tttcaatcgg cacgttgacc aaattacccc
      361 attctgtcca tgaaccgtca tagttgcgga cattgggata gccaagaagg taggtcagca
      421 caaaccaact gtggctggag cgttcaccga tacgacaata agtaacaatg tctttatcgg
      481 ctgtgatgcc aacagactca tacaattcct tgagttcttc cgcagattta aatgtgccat
      541 cttcgcgcac tgcctttgcc cacggaatat tctttgcgcc ggggatgtgc ccgccgcgta
      601 atgcgccttc ctgtggatat tccggcatgt gcaatttttt accgctgaat tcatctggtg
      661 aacgcacatc cactaacgga agattgtttt tgacatgctt caacacatcc tcacggaaga
      721 cgcggatctt tttgtcatcg cgcttgggtg ccttgtagac ggtttttgtg tagctgggaa
      781 cttcttttgt gaattcccgc ttttcgtctt cccatttctt acgtccgcca tttaggatgc
      841 gcacatcctt gtgaccgaag agcttgaaga cccagaagtt atacgttgcc caccaattgt
      901 ttttatcgcc gtagaagaca acggtggtgt cattgtcaat gcctttttca gaaagcaatg
      961 aggcgaactg aatagagtcc aaataatcgc ggacgatagg gtggttgagg tcttcatgcc
     1021 aggtgatctt gaccgcgccg ggaatgtgac cggtatcgta gagtagaaaa tcttcgtccg
     1081 actcgatgat gcggactttg gggtcgtgca ggtgttgagc gagccagtct gtttcgacca
     1141 gcacctcagg gtgggtatac gttgacatgg tttatctcct tttaattatt attcctattg
     1201 acttagtagg gtataaaact aaaaaagaat gaccttgtct ggtcatagtt ctttatatta
     1261 ctaattcaat aggatttatt gaataaatca taattcagcg attgaatttt gtcaacaccc
     1321 ttttgataac tcaaacagcc ctttcttaac agttttggcg accaaatcta atgtacaaga
     1381 ttacattaag agtagaatag tccaaattta tattgtaagg cttactttgt tcagaaacct
     1441 tagcatggag aatttattgt atgaagaaca agtacctgaa attccttttc cccattgcac
     1501 ttattctagc cctgactttg tccatgatcg gcactcgaag tgtcctggca gatgatgcgc
     1561 ctcctacccc cacagacgaa gttccaactg aaacacctcc agtcgaggaa ccttctgaga
     1621 ctcaagaacc gttggagcag actgttccag aaattttaga acaattacca gctgagacag
     1681 agatcgttgt tctcgatgag aatagcgaac ctttgccact ggtttcgaat gaagctgcca
     1741 atgttttggt gaccggtgat ccacaatggt gcccagcagg tgtcacaccc ggaactgata
     1801 ctctgagtca gtgtacagtt gcgcacgcca gcttcaatga tctgatcaat gacttgcaaa
     1861 caaatagtgc tacatattat ggctcaggta cgatct
//
LOCUS       DAOQJE010000766         1894 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_51153_length_1894_cov_4.720500, whole genome shotgun sequence.
ACCESSION   DAOQJE010000766 DAOQJE010000000
VERSION     DAOQJE010000766.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1894)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1894)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1894
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_51153_length_1894_cov_4.720500"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..421)
                     /locus_tag="PLT08_16280"
     CDS             complement(<1..421)
                     /locus_tag="PLT08_16280"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014612531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HNE06085.1"
                     /translation="MSRDKHAGLLNHHIKSPNSEYRMSNSSFQEIILKLQDFWASKGC
                     LITQPYYTQVGAGTMNPATFLRVLGPEPWNVAYVEPSVRPDDGRYGENPNRFQLHTQF
                     QVILKPDPGNPQELYLESLKALGIDPRQHDIRFVEDNW"
     gene            complement(547..>1894)
                     /locus_tag="PLT08_16285"
     CDS             complement(547..>1894)
                     /locus_tag="PLT08_16285"
                     /inference="COORDINATES: protein motif:HMM:NF015722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HNE06086.1"
                     /translation="LDVAMEKPEGEIFIIPVRLEECEVIKSLREWHWVDLFESDGYEM
                     LMRALHARANNIGATLQIKKSWLPIKEKQPAITQKESEEISKPKNLENDDVVKQAWKN
                     ASQVYDKTIEENNHYIASLERKRKWNKFVSEILFKFRIYAPFRIYILVVVIGLVYLSS
                     YFIKNMPLQKAIPTEAVTLTQQIISTSTKVLTPTKTFQPALTSTQVITLTPTSLPTEI
                     TDAKGVPMALVPAGEFIMGSDVGRDDEKPAHTVYLDAYYIDVNEVTNAVYKACVDVGA
                     CIPPENTSSYTRLNYYNNALYDDYPVVYVDWNMAKTYCEWRGASLPTESQWEKAAGGG
                     LGFTYPWGGDIDCNKANYFTCKADTTKVGSYESGRSPYGLYDMAGNVSEWVVNWFDAY
                     PKNIKINSSYGTEYRVLRGGSWNFSGFDLRTSARSHYRPLNSEYFIGFRCAKDLVP"
BASE COUNT          510 a          406 c          405 g          573 t
ORIGIN      
        1 cccagttatc ttccacaaag cggatgtcgt gctggcgcgg gtcgatgccg agggctttga
       61 gcgactcgag atacaactct tgcggattgc ccggatcggg cttgaggatg acctgaaatt
      121 gggtgtggag ttggaagcgg ttggggttct cgccgtaacg cccgtcgtcg gggcgcacag
      181 acggctcgac gtaggcgacg ttccacggtt cgggtccgag cacgcgcagg aaggtggcag
      241 gattcatcgt ccctgcaccg acctgggtgt agtatggctg tgtgatgaga catcccttgg
      301 atgcccagaa gtcttgtagt ttgaggatga tttcttggaa ggaagagttc gacattcgat
      361 attcgctatt cggggatttg atgtggtggt tgagtagtcc cgcatgtttg tcgcgggaca
      421 tatcgaaacc cgggaggatt ataactgatg gttaatggaa gatgcagaat gggatcttgt
      481 cccttatcta tagagtatgg ttcagattta agggagttat tatgtgacaa tctattttgg
      541 tcaattttag ggaactaaat ccttggcaca acgaaaacca ataaagtatt cgctattaag
      601 gggacgataa tggctacggg cagaagtacg caaatcaaat ccgctgaaat tccatgaacc
      661 accacgcaaa acacgatatt ctgttccata cgatgaatta atcttgatgt ttttagggta
      721 agcatcaaac caattcacaa cccattcaga aacgtttccc gccatatcat atagtccata
      781 tgggctcctt ccgctttcat aactgccaac cttagtcgta tctgctttac atgtaaagta
      841 atttgcttta ttgcagtcaa tatctccgcc ccatggataa gtaaatccaa gtccccctcc
      901 cgcagctttt tcccattgag attcggtggg caggctggct ccacgccatt cacagtaagt
      961 ctttgccata ttccaatcca cataaacaac gggataatca tcatacaacg cattattata
     1021 ataatttaaa cgagtgtaag aacttgtgtt ttctggagga atgcaagcac ctacatctac
     1081 acaagcttta tatacagcat tagtcacttc atttacatct atgtaatatg catcaaggta
     1141 cacagtatgt gcaggttttt catcatctct cccaacatcg ctccccatta taaattcacc
     1201 cgctggcaca agcgccattg gcacgccttt ggcatccgtg atttctgtgg gcaaagatgt
     1261 aggcgtcaat gtaataactt gagttgaggt caaagctggc tgaaaagttt ttgttggggt
     1321 taagactttt gtagaagtag aaataatttg ctgggtaaga gtaaccgcct ctgtggggat
     1381 cgccttttgc aaaggcatgt tctttataaa atatgaggat aggtaaacaa gaccaatgac
     1441 tacaaccaaa atatatattc tgaatggcgc ataaatacga aatttaaaaa gaatttctga
     1501 tacgaattta ttccattttc gttttctttc aagcgaagct atatagtggt tgttttcttc
     1561 aatcgttttg tcgtacactt ggctggcatt tttccacgcc tgttttacaa catcatcatt
     1621 ttctaaattt ttaggttttg atatttcttc agattctttt tgagtaatag caggttgctt
     1681 ttctttaatc ggcaaccaac ttttctttat ttgcaaagtt gccccaatat tatttgcccg
     1741 tgcatgtaag gcacgcatga gcatttcata tccatcgctt tcaaataaat ccacccaatg
     1801 ccattctctt aaactcttga taacctcaca ctcttctagt cgaacaggaa taataaatat
     1861 ctcaccctca ggtttttcca ttgcaacatc aagt
//
LOCUS       DAOQJE010000767         1892 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_51265_length_1892_cov_6.985302, whole genome shotgun sequence.
ACCESSION   DAOQJE010000767 DAOQJE010000000
VERSION     DAOQJE010000767.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1892)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1892)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1892
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_51265_length_1892_cov_6.985302"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..454
                     /locus_tag="PLT08_16290"
     CDS             <1..454
                     /locus_tag="PLT08_16290"
                     /inference="COORDINATES: protein motif:HMM:NF022853.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="protein-disulfide reductase DsbD family protein"
                     /protein_id="HNE06087.1"
                     /translation="AYPLKLATFDKNSVSVLIHLEKDADENYFLSATFTPEQGFHLYS
                     KDLPITGANGLGRPTLIEAGVESQYQVTGKLLESVPAEIPEFEPKDLLVYPVGAVTLR
                     LPIELPPGNGWTDDSVKITYMACSDRGCKAPVIGQEIAIRIPGKETFH"
     gene            477..1043
                     /locus_tag="PLT08_16295"
     CDS             477..1043
                     /locus_tag="PLT08_16295"
                     /inference="COORDINATES: protein motif:HMM:NF012314.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HNE06088.1"
                     /translation="MKKYSLILIALLGALACKALTPNPQEVETPVATDEPPSVIVQIT
                     DAPSEETAVTSSTGFTIVRLKPEDGDLATMLESESKKAQALGQMPVVEFDATWCPPCQ
                     AIDQSIGEENELMMNAYDGTYIIKLDVDVWGWDNGKVEMFQFEAIPVYFKLDTSGKPN
                     GEVIDGGAWGEDIPENIAPVMDAFFHNK"
     gene            complement(1091..1819)
                     /locus_tag="PLT08_16300"
     CDS             complement(1091..1819)
                     /locus_tag="PLT08_16300"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNE06089.1"
                     /translation="MSDIALEVRDLVKTFSLDGSTINAVDHVSFQVKFGEFVALVGPS
                     GSGKTTMLSILAALLTPTSGQVLIDGQDLSKMSEKERVKLRREKIGFTFQANNLIPYL
                     SARENVEFMLRLNGKLDHASRIRSDELLARLGLAERLHNLPAQMSGGQQQRVAIARAL
                     IHNPALVLADEPTASLDTERAFQVVETFANLIHENGRAGIIVTHDLRMCQYVDRVFQM
                     QDGKLIRTYETKNEIMELATATVH"
BASE COUNT          544 a          412 c          452 g          484 t
ORIGIN      
        1 agcatatccg cttaaattgg caactttcga caaaaattcg gtaagcgtct taatccactt
       61 ggaaaaagac gcggacgaaa attatttcct ttctgctacg tttaccccag agcaagggtt
      121 tcatctatat agtaaagacc tacccatcac aggggcaaat ggacttggac gtccaacatt
      181 aatagaagct ggcgttgaaa gtcagtatca agtcacgggg aaattacttg aaagtgtacc
      241 agccgaaatt ccagaatttg agccgaagga tcttttggtt tatcctgttg gggcagttac
      301 cttgcggttg cccattgagc ttccacctgg aaatggttgg acagacgatt cagtgaaaat
      361 cacctacatg gcttgttcag accgtggctg taaagcgccg gttattggtc aagagattgc
      421 aattcgtatc cccggcaaag agacgtttca ctaattctat aaaagatggg agacttatga
      481 aaaaatatag tttgatattg attgctcttc taggagcatt ggcatgtaaa gcactaacgc
      541 caaatccaca agaagtagag actccagtcg ctacagatga accgccgtcc gtgattgttc
      601 aaatcacaga cgcaccatcg gaagaaactg ctgtaacatc gtctactggg tttaccattg
      661 tgcggctcaa gccagaggat ggagacttag cgacgatgct agaaagcgaa agtaaaaaag
      721 cacaagcgct gggtcagatg cctgtcgttg agtttgatgc gacctggtgc ccaccttgtc
      781 aggcaattga ccagtctatt ggtgaagaga acgaattaat gatgaacgcc tacgatggga
      841 cctatatcat caagttggat gttgatgtct ggggttggga taatggcaaa gtagaaatgt
      901 tccaatttga agctatccct gtatatttca agctggatac ttctggaaaa ccaaacggtg
      961 aagtgattga tggcggtgca tggggcgagg atattcccga aaacatagca cctgtcatgg
     1021 atgccttttt tcacaacaaa taataaaaag ggctcacttt tgaagtgagc cctttttatt
     1081 tataaccaat ttagtgaact gtcgctgtag ccagttccat aatttcgttt ttagtctcat
     1141 acgtgcggat cagtttaccg tcttgcatct ggaagacacg gtctacatat tggcacatac
     1201 gcaagtcgtg cgtcacgatg ataccggcac gtccattttc atggatgaga tttgcaaagg
     1261 tttcaactac ttggaaggca cgctcagtat cgagtgaagc ggtgggttca tcggcaagta
     1321 caagtgcagg gttatgaatc aacgcccggg cgatagcaac acgttgctgc tgaccaccag
     1381 acatttgtgc aggcaagttg tgtaagcgtt ccgccaaccc aagccgagca agtaattcat
     1441 cagagcggat gcggctggca tgatccagct tgccgttgag gcgcagcata aattccacat
     1501 tctcacgggc agaaagatat ggaattaaat tattcgcctg aaaggtaaaa ccgatcttct
     1561 cgcggcgcag tttgacacgt tctttctcgc tcatctttga aaggtcttgc ccatcaatga
     1621 ggacttgtcc gctagtgggt gtaagtaagg cagcaaggat ggagagcata gtcgttttac
     1681 cggaaccact aggaccgacc aacgccacaa actcaccaaa ctttacctga aacgatacat
     1741 gatctactgc gttgatggtc gagccatcga gtgagaaggt tttgactaaa tcgcgaactt
     1801 ccaatgcaat atctgacata tttaccctaa cctaagtgcc ttgagcggct caatacgaac
     1861 agcataaatg atagagacca ttccaccgat cg
//
LOCUS       DAOQJE010000768         1891 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_51327_length_1891_cov_4.147603, whole genome shotgun sequence.
ACCESSION   DAOQJE010000768 DAOQJE010000000
VERSION     DAOQJE010000768.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1891)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1891)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1891
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_51327_length_1891_cov_4.147603"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(274..618)
                     /locus_tag="PLT08_16305"
     CDS             complement(274..618)
                     /locus_tag="PLT08_16305"
                     /inference="COORDINATES: protein motif:HMM:NF017757.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HNE06090.1"
                     /translation="MSRLSSPVQVPSGRQVQLHKAVQRYLNALPADASAKSVESFKVL
                     MDVGLTREFKQLRHLQGDLWELKVDAKSVKFMRFLFVARNGEFLFTHAFRKQTNQTPR
                     REIDLALRRANE"
     gene            complement(1005..1298)
                     /locus_tag="PLT08_16310"
     CDS             complement(1005..1298)
                     /locus_tag="PLT08_16310"
                     /inference="COORDINATES: protein motif:HMM:NF025120.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HNE06091.1"
                     /translation="MFTSFDDYVKDKLQKDQEFAKEYERLELPSMLARQVIKLRVEQG
                     ITQAQLAKLVGTQQSSISRLESMTALPSLSFLQKIAEVLNARIEIRLVPNPKA"
     gene            complement(1657..>1891)
                     /locus_tag="PLT08_16315"
     CDS             complement(1657..>1891)
                     /locus_tag="PLT08_16315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE06092.1"
                     /translation="PLDKYHKIVILSRQLEKVPYQQSYTTERRKVLWTIYSSTWRVNG
                     THIVKFQGNNIRFHPRVPGPVITKGTSRSNQTN"
BASE COUNT          447 a          379 c          463 g          602 t
ORIGIN      
        1 gattggtgtt gccggcgaga aaaccgctgg tgtacacacc actggaagat gggtcttggc
       61 gggaagacta cagcggaagg gtgatctcac atgaacggtt gcaacaatat cttgtaaagt
      121 atggatttat ataaacagtg tcctgctcaa agagcgactg gattaatgca aaacaagcga
      181 gaaacctcgc ttgttttgtt ttaaccatat accttcacta tcttcacaga gaattagaac
      241 ggagggatgt tgacacatcc ccccggaaat tgcctactcg tttgcccgac gcagtgcgag
      301 gtcgatttcg cgacgcgggg tttggtttgt ctgctttctg aaggcgtggg tgaacaggaa
      361 ttccccattc cgcgctacga acaagaagcg catgaatttg acgcttttcg cgtcaacctt
      421 gagctcccac aagtcacctt gtaagtgacg gagctgtttg aactcacggg ttaatcctac
      481 atccatgagt actttgaagg actcgacgga ctttgccgac gcatcagcgg ggagtgcatt
      541 gaggtagcgt tggaccgctt tgtgcagttg gacttggcgc ccgctgggca cctggacggg
      601 ggatgagaga cgactcactg ggacctcctt ttatttgggt ggacatgaca acacaaatct
      661 tttcgaaagc gcaggtacat aacgcttgat ctaataggct tgcggtcagc ctcccattaa
      721 gaactttgct tcaagggcaa cctcctttct ttgatctaga tctcaaaaat tcagcgaata
      781 cccaaagtgc aaaatcgtta taaatagagg ggagctacag tctctctgtg tcagatattt
      841 ggatgtgaag gtacaaagtt tccgctttat tgtgccaaaa acaggcagac ggaataagtg
      901 ctatttttcc tgtactacct gttttttgtc acaaccaaac caaaaaatta ttttcttcca
      961 ataacagatc tgatttggtg ttctgaatat ttagttgtca aaaactatgc ttttgggttg
     1021 gggactagcc tgatttctat ccgagcattt aatacttctg ctattttctg taggaagctg
     1081 agtgatggta gagcggtcat actttccagg cgggagatgc tggattgctg cgtgccgacg
     1141 agttttgcca attgagcttg cgtgatgcct tgctccaccc gcaatttgat gacctggcgc
     1201 gcgagcatac tgggcaattc gagcctttcg tattctttcg cgaactcctg atctttctgg
     1261 agtttatcct ttacatagtc atcgaagctg gtgaacatga tatttaatct ccaaaatgtt
     1321 ttatatgaat tatgttttat tatgatcttt tatgtaatta agtaattcta ttttcaagag
     1381 gggtgagggc gggttttcta cccgccccct tgttcttgga atattcgatt gttaagttag
     1441 tgtttcgttc gtttcagcgc ctgttttttg tgcttttttg gtatttggtc gctggttttt
     1501 ctgaagctgt agtttatatg tatgtttctg ttctcatcga tgtagtagaa cagccggaat
     1561 atttttctgt tgcatcttgc tcggagttcc caggttttgt cctttatatg gcggacgtgt
     1621 gggtatgaaa gttgggttcc gaacatttcg aggagttcaa tttgtctggt tactgcggct
     1681 cgttcctttg gtgataactg gtccaggaac tcttgggtgg aagcggatat tattgccttg
     1741 aaacttgacg atatgggttc cattgaccct ccacgtagaa gaatagattg tccataagac
     1801 ttttctcctt tctgtagtat aggattgttg gtaaggtact ttttccaact ggcgagataa
     1861 tatcacaatt ttgtgatatt tgtcaagggg g
//
LOCUS       DAOQJE010000769         1890 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_51365_length_1890_cov_5.609264, whole genome shotgun sequence.
ACCESSION   DAOQJE010000769 DAOQJE010000000
VERSION     DAOQJE010000769.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1890)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1890)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1890
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_51365_length_1890_cov_5.609264"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..859)
                     /locus_tag="PLT08_16320"
     CDS             complement(<1..859)
                     /locus_tag="PLT08_16320"
                     /inference="COORDINATES: protein motif:HMM:NF013434.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HNE06093.1"
                     /translation="MPTTDMLKRDVIIIGGGPVGLSCAYYLLKSGRQVTLLDAKEIGK
                     GSGSGNAGHIVPSHIIPLAAPGVVTSALKWMLDPAHSPFGMKVSLNPNYLMWLLRFTI
                     ACSQNNVDRSLEPLNQLGQLSAKNFAQIIAEEKFDCAYQEMGLLHLFKTEKAFHEGQH
                     EGEFMQRHGIPVSVYDKNKIHEVEPAAVDDVLGGVHFTGDAHLNPAVFLNLLKERVKS
                     MGVELMDGTQVTGFETANGKITKVKTSAGDFEAEQIVLAAGALTPSVAKDLKLNIPVQ
                     PARGYSMTMS"
     gene            1068..1472
                     /locus_tag="PLT08_16325"
     CDS             1068..1472
                     /locus_tag="PLT08_16325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE06094.1"
                     /translation="MKKFNIKSLLVFVVALIMVLSLSACATEEVSDASATDEAATEVA
                     TEVATEVATEEAGVIVPEFTFNNTGEEEICEIYLSPVGQDQWGPDQLGGATIPSGGSY
                     VLQNIPAGQYDVKAVGCAGSEVIGQLDIQPQQ"
BASE COUNT          493 a          480 c          391 g          526 t
ORIGIN      
        1 cggacatggt catactgtat ccacgtgcag gctgaaccgg aatgttcaac ttcaaatctt
       61 tggcaacgga aggagtcagc gcgccagcgg caagcacgat ctgttctgct tcaaaatctc
      121 cagcagacgt tttgactttc gtaattttgc catttgcagt ttcaaagccc gtgacctgcg
      181 taccgtccat caactccaca cccatagatt tgacacgttc cttcagcaag ttgagaaata
      241 ccgccgggtt taggtgcgca tctcccgtga aatgcacacc gccaaggaca tcgtccacgg
      301 cggcgggctc gacttcatga attttgtttt tgtcatatac actgacagga atgccatgtc
      361 tctgcatgaa ttcgccttcg tgctgaccct catggaaggc tttctcggtt ttgaataaat
      421 gcagtagtcc catctcttga taggcacaat caaatttttc ttcggcaatg atctgagcaa
      481 aattcttcgc gctaagttga ccaagttgat tcagaggctc caatgagcga tccacattgt
      541 tctggctaca cgctatcgta aaacgcaata gccacatcaa gtaatttggg ttgaggctaa
      601 ctttcatccc aaagggacta tgtgccgggt caagcatcca tttaagggca gaggtcacca
      661 caccaggtgc agcgagtgga atgatgtgac tcggtacgat atgcccggca ttgcctgaac
      721 cgctgccttt accaatctct tttgcatcca gcagagtcac ttgcctgccc gatttcaaaa
      781 ggtaatacgc acaacttaac cccacgggtc ccccaccgat gataatcaca tcgcgtttca
      841 acatatctgt tgtaggcatc gttatcttct ccaaagatca ttcaagtttg aatatttcaa
      901 gtcattatat tccagagccg cttgagtcta tgttgaccga atccaaatat gagaaccatt
      961 aaaaagattc caaattaata attttataat caaaaatagg ttgcactttt ttctttttta
     1021 tgtataatcc gcgcccgcac gtaatctaaa caacataaag gaatacaatg aagaagttca
     1081 acatcaagtc tttgctcgtg ttcgtcgtcg ccctcatcat ggttctcagc ctttctgcat
     1141 gcgcaactga agaagtcagc gatgcttccg ctactgatga agctgccacc gaagttgcca
     1201 ctgaagtggc taccgaggtc gctactgaag aagcaggtgt gatcgttcct gaattcacct
     1261 ttaataacac tggcgaagaa gaaatctgcg agatctacct ctcccctgtc ggtcaggatc
     1321 aatggggtcc cgatcagctc ggtggcgcca ccatcccctc cggtggttcc tatgttctcc
     1381 agaacatccc cgctggtcag tatgatgtca aagccgttgg ctgcgctggc tccgaagtga
     1441 tcggtcagct cgacattcaa ccgcaacaat aattgaatga cagaaaagac ctctgagttt
     1501 ttctcagagg tctttttatt tatgacatat ccgctctaaa ttcactcgct atatgccatt
     1561 ataatgagcg gcgctctaaa acatgggatg ggttgtaaag gtttcttgca attgcataac
     1621 cctgtcttga caaacccacg aacccatgaa taattcactg aatttctttg aaacatttca
     1681 gccaaagggt gtttctaaaa ttaacttttc aaaggtctga aaacgttcca atgcaatggc
     1741 aaactaaccc atattttgct ccaattatca tttccagcct gatcgccatc gtctgtgcat
     1801 tttttgtcat cacgcgcaga aaccgggcat ctggctcgac agcgctcctc gggttgatct
     1861 tttccgtcat catttgggga tttgcctacg
//
LOCUS       DAOQJE010000770         1888 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_51480_length_1888_cov_6.303328, whole genome shotgun sequence.
ACCESSION   DAOQJE010000770 DAOQJE010000000
VERSION     DAOQJE010000770.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1888)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1888)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1888
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_51480_length_1888_cov_6.303328"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..451)
                     /locus_tag="PLT08_16330"
     CDS             complement(<1..451)
                     /locus_tag="PLT08_16330"
                     /inference="COORDINATES: protein motif:HMM:NF024248.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HNE06095.1"
                     /translation="MNEMLDYVKALSDPERLRIIGLLSQGNATREEIATRLNLSPKDS
                     LTHLGFLEFVGVVTQTDGIFALNNDKLATLGKEKLFNGGNKFIPPEDMDEASKKILRD
                     FLNADGTIRQVPEQKKIGPILDYLIKNFEFDRTYTEREVNIIIKQFNE"
     gene            680..1600
                     /locus_tag="PLT08_16335"
     CDS             680..1600
                     /locus_tag="PLT08_16335"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: protein motif:HMM:NF005146.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004084 - branched-chain-amino-acid
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009081 - branched-chain amino acid
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transaminase"
                     /protein_id="HNE06096.1"
                     /translation="MDLTKHAFFEGKIVPLGEAKINIATHGFLYGTAVFSGMRAYWNE
                     EKKGLYLFRPYDHFRRLLDSARMMAMEVPYDEEGLIQLTIDLLKTDNYQQDVYLRPTI
                     YKADMGIGVRLHNLRDELSIFTAAYEPYVKNDTNSHVTVSSWRRVDDNAIPARSKVAG
                     AYVNSALAKTDANRAGFDEALVLTSEGHISEGSAMNVFMVRNGTLYTPPLTDNILEGV
                     TRRSVIEVAQKELGIEVVERSIDRTEILIADEVFLTGSAAQVVAVTKIDYRPIGSGVM
                     GPITSKLRDIYEDIVRARNSKYANWNVIVK"
     gene            complement(1637..>1888)
                     /locus_tag="PLT08_16340"
     CDS             complement(1637..>1888)
                     /locus_tag="PLT08_16340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE06097.1"
                     /translation="IAVRLTGFYNGKSKNELTVSGIAPAYGKSGFELFLSAVPIDSDN
                     LLTIQILDQAGLPLSDNIPIKTYADCTKNMVLVKFKKNP"
BASE COUNT          513 a          407 c          422 g          546 t
ORIGIN      
        1 cttcattgaa ttgtttgatg atgatgttaa cttctcgttc tgtgtaggtt ctatcaaact
       61 caaagttttt gattaagtaa tccaatatag gtccaatctt tttttgctct ggtacctgac
      121 gaatcgttcc atctgcattt aaaaaatctc gcagaatctt ttttgaagcc tcatccatat
      181 cttctggcgg aataaattta ttaccgccat tgaatagctt ttcttttcca agcgtggcga
      241 gtttatcgtt attaagcgca aaaataccgt cagtttgtgt aacaacaccc acaaattcaa
      301 gaaagcccaa atgggttaat gaatctttgg gcgagaggtt taaccgcgtc gcaatttctt
      361 cgcgcgtggc atttccctga gaaagcagac caatgatccg caagcgttcc gggtctgata
      421 aggctttgac ataatccagc atttcattca tatttcactt ccgtagattt atttcgacag
      481 ttatcgaatt agattttagg agggaatcca cggtttgtca agggataagg tccgctacca
      541 gcgtgagagc ggcttggtct gtagttccaa gcctgagcgc cgcccgaagc ggactctctg
      601 agtcggtgtt atacatggtg gtatgcaatg cccgcactgg gtataatcct gaccacaact
      661 cagcacaaag gaggtgcaag tggatctcac aaagcacgct ttttttgaag ggaaaattgt
      721 tcccttgggc gaggcaaaga tcaacattgc cacgcatggt tttttatatg gaacggctgt
      781 ctttagcgga atgcgcgctt attggaatga agaaaaaaaa gggctttatc tctttcgccc
      841 ttatgaccat tttcgccgtt tgctcgattc tgcgcgaatg atggcaatgg aagttcctta
      901 tgatgaagag gggttgatac aacttaccat cgatcttttg aaaacagata actatcaaca
      961 ggatgtttat ttacgtccaa caatctataa agcagatatg ggcattggtg tgcgattgca
     1021 caacctacgc gatgaactct cgatctttac cgcagcctat gaaccctatg tcaaaaatga
     1081 taccaactcg catgtcacag tttcttcctg gcggcgcgtg gacgataatg ccattccggc
     1141 gcgcagtaaa gtagccgggg cttatgtcaa ctctgcgctg gcaaagactg acgcaaatcg
     1201 tgccgggttt gatgaagctt tggtattaac atcagaaggt catatctctg aggggtctgc
     1261 catgaatgtg ttcatggtcc gcaacggcac gctgtacact cccccactta ccgataacat
     1321 ccttgaaggc gttacccgtc gttccgtgat tgaagtggct caaaaagaat taggcattga
     1381 agtcgtagag cgttccattg atcgcaccga gattttgatt gcggatgaag tcttcttaac
     1441 tggcagtgca gctcaggtcg tcgctgtaac aaagatcgac tatcgcccca ttgggagcgg
     1501 tgtaatggga ccgatcactt caaagttacg agatatctat gaagatatag tgcgtgccag
     1561 aaattcaaaa tatgcaaatt ggaacgtaat tgtgaagtaa ataaaaaatc cgccgaatat
     1621 tcggcggatt ttttatttat ggattcttct tgaacttcac caacaccatg ttctttgtac
     1681 aatctgcata ggttttaatc ggaatgttat ccgaaagcgg caaacctgcc tgatcaagaa
     1741 tttgaatcgt tagtaaatta tcagagtcaa tgggcaccgc acttaagaac aattcgaaac
     1801 ctgatttccc gtatgcagga gcaataccgc tcactgtaag ttcgttcttg cttttaccgt
     1861 tatagaatcc tgtaagccgg acagcaat
//
LOCUS       DAOQJE010000771         1888 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_51485_length_1888_cov_4.256956, whole genome shotgun sequence.
ACCESSION   DAOQJE010000771 DAOQJE010000000
VERSION     DAOQJE010000771.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1888)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1888)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1888
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_51485_length_1888_cov_4.256956"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..304
                     /locus_tag="PLT08_16345"
     CDS             <1..304
                     /locus_tag="PLT08_16345"
                     /inference="COORDINATES: protein motif:HMM:NF025054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNE06098.1"
                     /translation="LLARNELGQVVGALNLTVYRVPTGIRAIIEDVIVDLSARGQGIG
                     EAIMQQAIEIAQQKGASNISLTSNPMRVAANKLYIKLGFEKRETNAYQMKFKKAAE"
     gene            complement(339..1019)
                     /locus_tag="PLT08_16350"
     CDS             complement(339..1019)
                     /locus_tag="PLT08_16350"
                     /inference="COORDINATES: protein motif:HMM:NF013702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HNE06099.1"
                     /translation="MKKFSLWFITTALRVYFWLTLRVDAPGGMDKVPVKGPLIIITNH
                     TGQVEVPVFATMLQPRKITGWGKAEAFENPFLNWVFGAWDIIPVHRGEADMTALKSAF
                     RMLEEGRIFGVAPEGTRNYTGILRRGLPGAAILALKSGVPVLPIAHWGGEKYIKNLKS
                     FKRTDFHIRLGEPFYVKADGKVTSEMRQEIVDEMMYRIASMMPKEYHGEYSDFSKSTG
                     KYLVNTNL"
     gene            complement(1026..>1888)
                     /locus_tag="PLT08_16355"
     CDS             complement(1026..>1888)
                     /locus_tag="PLT08_16355"
                     /inference="COORDINATES: protein motif:HMM:TIGR00147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="diacylglycerol kinase family lipid kinase"
                     /protein_id="HNE06100.1"
                     /translation="PQIESFLKEHGFDFTLVRTTGVWHAADLAESAARDGYDVIVCAS
                     GDGTVNEAINGIMRAKKAGHTSAAFTVLSIGTGNDFAGGTGIPTNLNDSLQALKANKR
                     KKIDLGVVKGGDYPEGRYFGNGIGVGFDAAVGNEAIKVRWTRGLLAYLIGVIKTVFLY
                     YNPAQVEIILDDKETISQRSLMISVMNGRRMGGGFQMAPESQPDDGLFDLCIAETASK
                     GRILQMIPHFINGTQKTLPEIQMKRAKKVSIQSMDATFPAHADGEFICLEGKNLTLEV
                     LPQELEIIHA"
BASE COUNT          494 a          486 c          417 g          491 t
ORIGIN      
        1 gctgcttgcc cgcaacgaac tcggacaggt cgtcggcgcc ctaaacctta ccgtttatcg
       61 cgtgccaact ggcatccgcg ccatcatcga agatgtcatc gtggacttgt ctgctcgggg
      121 gcaggggatt ggtgaagcca tcatgcaaca agcaattgag attgcgcaac aaaaaggcgc
      181 cagcaatatc tcgctaacct caaatccaat gcgagtggcg gcaaacaaat tgtatattaa
      241 gttgggcttt gaaaaacgcg aaacaaatgc ctatcaaatg aaattcaaga aagctgctga
      301 ataaataaaa accggctctg ccatgagccg gtttttattt atagatttgt gttgaccaga
      361 tatttccctg ttgatttact gaaatcacta tattcgccgt gatattcttt cggcatcatg
      421 gatgcgatcc tgtacatcat ttcgtccacg atctcttggc gcatttcact ggtcactttg
      481 ccatctgcct tcacatagaa aggctcgcca aggcgaatgt gaaaatctgt ccgcttgaag
      541 ctctttaggt ttttgatata tttctcgcca ccccaatgag caatgggcaa aaccggcacg
      601 ccagatttca atgccaaaat ggcagcacct ggcaacccgc ggcgtagaat gccggtgtag
      661 ttacgtgtcc cttcaggggc aacgccgaag atgcgacctt cttccagcat acgaaaagca
      721 gatttaagtg cggtcatatc tgcttcaccg cgatgaacag ggatgatatc ccatgcgcca
      781 aatacccaat tcaaaaatgg gttttcaaaa gcttctgctt ttccccaacc ggtgatcttt
      841 cgtggctgga gcatggtcgc gaaaacgggc acttccacct gcccggtgtg attggtgatg
      901 atgattaacg gtcctttgac agggactttg tccatgccac cgggggcgtc aacgcgcagg
      961 gtaagccaga agtaaacacg aagagctgtt gtaataaacc aaagagaaaa ttttttcata
     1021 agatattaag cgtgaatgat ctcaagttcc tgtggcaaaa cctcaagggt taaattcttc
     1081 ccttcgaggc agataaattc tccatcagca tgagccggga aggtggcatc catagactgg
     1141 atcgatacct tcttagctcg tttcatttga atctcgggca aggtcttttg cgttccatta
     1201 ataaagtggg gaatcatttg caaaatccgt cccttcgaag cagtctctgc gatgcaaagg
     1261 tcgaacaaac catcatccgg ctgagattcg ggcgccatct gaaaaccgcc gcccatgcgt
     1321 cgtccgttca tcacggatat catcaacgaa cgttggctga tagtctcttt atcatccaaa
     1381 ataatttcga cttgcgcagg gttgtaataa agaaaaaccg ttttgatcac accaatgaga
     1441 tatgccaaca agccgcgcgt ccaacgcact ttgatcgctt catttccaac tgccgcatca
     1501 aacccaacgc caatgccatt cccaaaataa cgaccctctg ggtaatcgcc gcctttcacc
     1561 acgcccaagt ctattttctt tcgcttgttt gctttcaagg cttgtagact gtcattcaaa
     1621 tttgtaggaa tgcccgttcc gcccgcaaag tcattccctg tgccaatgct caatactgtg
     1681 aatgcggcgc tagtatgccc agctttttta gcacgcatga ttccgttgat ggcttcattt
     1741 accgtgccat ctccgctggc acaaacgatt acgtcatagc catctcgtgc ggcactttca
     1801 gctaagtccg ctgcatgcca aaccccagtt gtgcgcacca aggtgaaatc aaagccgtgc
     1861 tcttttagaa atgattcaat ctgtggta
//
LOCUS       DAOQJE010000772         1883 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_51735_length_1883_cov_4.516958, whole genome shotgun sequence.
ACCESSION   DAOQJE010000772 DAOQJE010000000
VERSION     DAOQJE010000772.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1883)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1883)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1883
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_51735_length_1883_cov_4.516958"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..814)
                     /locus_tag="PLT08_16360"
     CDS             complement(<1..814)
                     /locus_tag="PLT08_16360"
                     /inference="COORDINATES: protein motif:HMM:NF028237.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase N-terminal 7TM
                     domain-containing protein"
                     /protein_id="HNE06101.1"
                     /translation="MTSSELAHIIPYLASFLISIFISGYAWTKRQAQGALGFELYMVG
                     QTVWIAGFILEMISKELGDKIFWDQVQWFCTTLIFVAIPIFAIQFTEHRIYFPRRYFF
                     LSLIVPMGFSFLLATNSIHHLIYNNPSLVASTPFSELHYDFTFVIYLYGLYGGAIALW
                     SGQLVFKRLIQPQSLYRAQALIVLLGFAIPLISWTLTLLGVQITPERDSTPFSAAIGN
                     LIIAWGIFRFHIFEVIPIARDRVFEALADPVVILDKNNIVIDINAAMLTLMDK"
     gene            complement(946..>1883)
                     /locus_tag="PLT08_16365"
     CDS             complement(946..>1883)
                     /locus_tag="PLT08_16365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HNE06102.1"
                     /translation="KGIGVAMVMQGTAIPYLDMGGASIKMNDDGSFNLLVGATDLGTG
                     SDTVIAQMAAEVLGVPIEDMITYSSDTDFTPFDKGAYASSTTYISGTAAVKAANIVAE
                     RIRIRAATMLEVADPNSIQLKDRHAHAPNGKSVSLAEIALDTLHKNNQEQIMGVASYV
                     SPVSPPPFAAQFAEVTVDTETGQVTVDRLVMAVDSGVIVNPLTASGQIEGGMTQALGY
                     AVCEEMRYDDKGVAYERDLDRYHIFRADEMPELETIFVETFEPSHPFGVKAVAEIPMD
                     GVAPAVGNAILDAIGVNVDVNPTTPERVWKAIKRQ"
BASE COUNT          519 a          472 c          418 g          474 t
ORIGIN      
        1 ttttgtccat caaggtaagc atggcggcgt tgatatcaat aacaatatta tttttatcga
       61 gaataaccac agggtctgcc aatgcttcaa aaaccctgtc tctggcgatg gggatcacct
      121 cgaaaatatg gaaacgaaaa atgccccagg caatgatcaa attgccgatg gcagcactaa
      181 acggggttga atctctttcg ggtgtgattt gaacgcctag caaggtgagc gtccaactta
      241 taagcggaat cgcgaaacca agaagaacaa tcagggcttg tgcgcgatat aaactttgag
      301 gttgaatcag acgcttgaat accaattgcc cagaccaaag ggcaatcgcc cctccataaa
      361 gcccatacag gtaaatcaca aatgtaaaat cgtaatgaag ctccgaaaaa ggcgtacttg
      421 ctacaagcga cggattgttg tatatcaaat ggtggatgct atttgttgct aacagaaacg
      481 agaaacccat cggcacaatg agcgatagga aaaaatatct tctagggaaa tagatgcggt
      541 gctctgtgaa ttgaatcgca aagattggga tagcaacaaa aatcaacgtt gtacaaaacc
      601 attgaacttg atcccaaaaa attttatccc ctagttcctt gcttatcatt tcaagaatga
      661 aaccagcgat ccatactgtt tgccccacca tgtaaagttc aaaccctaaa gccccttgcg
      721 cctgcctttt ggtccatgca taacctgata tgaatatgga tatcaagaag gaagcaagat
      781 agggaatgat gtgagcaagt tctgaactgg tcatgtattg tggcgttgat tatatctcgc
      841 tgtctttgta tgtacgagac aaaattattt ttgtaatttt acagaaaatg aggcgcggac
      901 aaatattgcc tcacgcctca ttctatcgtt tatgtctgtt tctgcttact gacgcttaat
      961 cgccttccag actctctctg gcgtggtggg gttcacgtcc acattgacac cgatggcatc
     1021 caagatggca ttcccaacgg caggagctac tccatccatc ggaatttccg ccactgcttt
     1081 cactccgaac ggatggctcg gctcaaaggt ctcgacgaaa atggtttcca actccggcat
     1141 ctcatcggca cggaagatgt gatatcgatc caaatcgcgt tcataagcga cgcctttatc
     1201 atcataccgc atctcttcac agacggcgta tccaagtgcc tgcgtcatac cgccttcgat
     1261 ctgtcccgaa gccgtaagcg gattcacgat cacacctgag tccactgcca tcaccaaacg
     1321 atccacggtg acctgacccg tttccgtgtc cacggtcact tcggcaaatt gtgcggcaaa
     1381 cggcggaggc gaaacgggtg aaacataact tgctacaccc ataatttgtt cttgattatt
     1441 tttgtgcagg gtatccaatg cgatttcagc caacgaaaca gactttccgt tcggcgcatg
     1501 agcatgacgg tcttttagtt gaatggagtt cgggtctgca acttccaaca tcgtcgccgc
     1561 gcggatgcga atcctctccg ccacgatgtt cgctgccttc accgccgccg tgccggaaat
     1621 ataagtcgta gacgaagcat atgcgccctt atcgaacgga gtgaaatccg tgtccgatga
     1681 atacgtgatc atgtcttcga tgggcacacc caatacttct gccgccattt gtgcaatgac
     1741 cgtgtctgaa ccggtgccca agtctgttgc accaaccaaa agattgaacg aaccgtcatc
     1801 attcatcttg atggatgcac cgcccatatc gaggtaagga atagctgtcc cttgcatgac
     1861 catcgccacg ccgatccctt tgc
//
LOCUS       DAOQJE010000773         1880 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_51873_length_1880_cov_6.948493, whole genome shotgun sequence.
ACCESSION   DAOQJE010000773 DAOQJE010000000
VERSION     DAOQJE010000773.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1880)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1880)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1880
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_51873_length_1880_cov_6.948493"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..931)
                     /locus_tag="PLT08_16370"
     CDS             complement(<1..931)
                     /locus_tag="PLT08_16370"
                     /inference="COORDINATES: protein motif:HMM:NF013918.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferric reductase-like transmembrane
                     domain-containing protein"
                     /protein_id="HNE06103.1"
                     /translation="MKRNLGNIVFTILVLLTIVVWVVFPPHSEGVENYLRYHIGMTMG
                     SVVIALMSFSLFISTRPRWAEPYFGGLDKMYMTHRYTSTAAFLLMFVHLLIVPLHLVN
                     LALGNYLAIVAFLGFIAIVLPTLAPRIPFLNKLTSGTYEGWKRLHGFIGIFFILGYLH
                     SITVNAPTTKIAINWNQIFVFLGIGSYIYTEVLSRFLGKHVPYTVKAVNHPNNSTTEV
                     VMQAKGKPIQHAHAGQFLFVRFKGDKILDEAHPFTISSAPHEGELRVTVKAVGDFTRY
                     LFQNLKPGMDAIVEGAYGMFNYKTGSDKQIWVAG"
     gene            complement(999..1412)
                     /locus_tag="PLT08_16375"
     CDS             complement(999..1412)
                     /locus_tag="PLT08_16375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HNE06104.1"
                     /translation="MEINISQEKGNVPVTVIQVTGQLDGQTYQKLIAAAQEVIKEGST
                     NILLDLSELTFISSAGLVSLHVIALLLRGEAMPDLDHGWAALKSVNKSRESGVQQHVK
                     LLNPREEVINVLDMVGFSSFFEVFKDKQKALESFS"
     gene            complement(1448..>1880)
                     /locus_tag="PLT08_16380"
     CDS             complement(1448..>1880)
                     /locus_tag="PLT08_16380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNE06105.1"
                     /translation="ILADPYNGRVEHGLDFIALGKLFEDLGHWDEAARLFERGLEIGL
                     DESDFGVAVKRISILQKKRGDMDRAVELWKAAAKKGHIYAHIELAKYYEHKMRDAKSA
                     VKWAKSARAEAEKADLPPYIRNHWFHEIDHRLERLTRKAGL"
BASE COUNT          527 a          491 c          391 g          471 t
ORIGIN      
        1 cgccagcgac ccaaatctgt ttgtcgctgc ctgttttgta attgaacatc ccatatgcgc
       61 cttctacaat cgcatccatg ccgggtttga gattctgaaa caggtaacgg gtgaaatcac
      121 cgacagcttt aaccgtcact cgcaattcgc cttcatgcgg cgcactcgaa atagtgaagg
      181 gatgggcttc atccagaatt ttatcgcctt tgaaacgcac aaacagaaac tgacccgcat
      241 gagcatgttg gatgggcttg cccttggctt gcatcaccac ctcagtggtg gagttattag
      301 gatgatttac cgctttcacc gtataaggca catgcttgcc taggaagcgg ctcaacactt
      361 ctgtgtaaat gtaggaacca ataccaagga aaacaaagat ctgattccag tttatggcaa
      421 tctttgttgt tggggcattc accgtgatcg agtgtagata acccaaaatg aagaaaatac
      481 caataaagcc atgcaaacgc ttccagcctt cataggttcc gctcgtgagt ttattcaaga
      541 acgggatgcg tggcgcgagc gtaggtaaaa cgatggcgat aaaaccgaga aatgcaacga
      601 tcgctagata atttccaagc gcaaggttta ccagatgcaa tggcacgatc aacaagtgaa
      661 caaacattag caagaatgcg gcagtcgatg tatagcgatg ggtcatgtac atcttatcca
      721 agccaccaaa gtatggctct gcccagcgcg gacgagtgga aatgaacagc gagaacgaca
      781 tcaaggcgat cacaacagac cccatcgtca tcccgatgtg atagcgtaga taattttcaa
      841 ccccttcact gtgcggcgga aagacaaccc acacgacgat ggtaagtaag accaaaatag
      901 taaagacaat attcccaaga ttacgtttca taaaaattca cctttaatat aaatgacaac
      961 gatgcaggca tgcctgcatc gttgtgggtc gtttcttctt acgagaaaga ttcgagagcc
     1021 ttttgtttat ctttgaaaac ctcaaagaaa gaagagaagc caaccatatc gagcacgttg
     1081 atcacttctt cgcgcgggtt cagcaatttc acgtgttgtt gaaccccact ctcacgtgat
     1141 ttgttgaccg acttcaacgc cgcccaacca tggtcaaggt ctggcatggc ttcgccgcgc
     1201 aacagcaggg caatcacatg cagcgacacc aaccctgcgc tggaaataaa agttagctca
     1261 cttagatcca ataaaatatt cgttgagcct tctttgataa cttcctgagc agcagcgatc
     1321 aatttctgat acgtctgccc gtctaattga cccgtcacct gaatcacagt aaccggcaca
     1381 ttcccctttt cttgtgaaat gttaatttcc atgtttcctc tccaaaggtt aatccgtgtt
     1441 cgagcaatta taaacccgct ttacgcgtca acctttccaa ccgatggtca atttcatgga
     1501 accaatgatt acgaatataa ggaggcaaat ccgctttctc ggcttctgcc cgtgcagact
     1561 tcgcccactt caccgcagac tttgcatcgc gcatcttatg ctcataatat tttgccaatt
     1621 caatatgagc ataaatatga cctttcttcg ccgccgcttt ccacaactcg acagcccggt
     1681 ccatatctcc gcgcttcttt tgcaggatgg agatccgctt cactgctacg ccgaaatctg
     1741 attcatccaa cccgatctcg agtccacgct cgaacagacg tgccgcctca tcccaatgtc
     1801 caaggtcttc aaatagtttt cccagtgcaa tgaaatcaag cccatgctcc acacgtccat
     1861 tataggggtc tgccaaaatc
//
LOCUS       DAOQJE010000774         1880 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_51874_length_1880_cov_6.926575, whole genome shotgun sequence.
ACCESSION   DAOQJE010000774 DAOQJE010000000
VERSION     DAOQJE010000774.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1880)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1880)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1880
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_51874_length_1880_cov_6.926575"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1440)
                     /locus_tag="PLT08_16385"
     CDS             complement(<1..1440)
                     /locus_tag="PLT08_16385"
                     /inference="COORDINATES: protein motif:HMM:NF013100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HNE06106.1"
                     /translation="MRQQYGRRSQVLAAALAFFAFAIVFQMTRIQNSPEADTFRQLSK
                     DYSYEWRTFYPNRGEIYDRNGRLLAGEKIVYEIGVDLNIMTDAHAIAFAVADALGMNY
                     DNLMSVIQNPPDGLSYIVLADYVESSQANYLQDLKKALNEQAPEGSMGGLTGLQFKSH
                     PQRSYPENSLASNILGFVNREGKGYFGVEEKYDSLLAGNPVTVLVPTDPNKAFEIPNA
                     PDGTTLVLTINRDLQSAAEEILDNALGTYGAQDGIIAIMDPQSGEMLAVATSRRMDLN
                     EFWNYGTIYNNATEFNPIISKTYEPGSVMKILTMAAALDSNTVTPETTYLDTGEILIG
                     GVTIQNWDRNPWGVQNMVGCLQHSLNICMVTISSQMGANTFYKYMDAFGIGHLTGVDL
                     AWEAPGRLKVPGDADWYPVDLGTNSFGQGVAVTPIQMMSAVSAVANNGRMVIPHVLYA
                     MVRDGRQYEVPDQFGPTPISEQTAHTLSEM"
     gene            complement(1437..>1880)
                     /locus_tag="PLT08_16390"
     CDS             complement(1437..>1880)
                     /locus_tag="PLT08_16390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE06107.1"
                     /translation="HVIHAYRVAPWRVQRQWIGNALLVVVSLAMVAALYLNVTSRTAI
                     AGREIQDLTDSIAVSQQVSSDLQTQLANLTSAEVMQQRAKEMGFRPVEPVELEYLVVP
                     GYVAKDAVNLSSSAQLQLNVLAIPPEYNQSLLDWMDERLVARRYQ"
BASE COUNT          510 a          455 c          442 g          473 t
ORIGIN      
        1 catttcagat aatgtatgtg cggtctgctc tgagatgggt gtaggaccga actgatctgg
       61 tacttcgtat tggcgaccat cacgcaccat cgcatacaac acgtgcggaa tgaccatacg
      121 cccattattg gctactgcag aaactgcaga catcatttga ataggagtga cagccacacc
      181 ttgaccaaat gagttggtac ccagatcaac tggataccag tccgcatctc ccgggacctt
      241 gagcctgccc ggtgcttccc aagccaagtc cacgccggtg aggtgcccaa ttccaaaagc
      301 gtccatgtat ttatagaaag tatttgcacc catttgtgaa gaaatggtga ccatacaaat
      361 attgagtgaa tgctgcaaac accccaccat gttctgcaca ccccacggat tgcgatccca
      421 gttttgaatg gtcacaccgc cgatcagaat ctcaccagtg tcgagataag tcgtttcagg
      481 ggttacagta ttgctgtcta aagcggcagc catcgtaagg atcttcatca cggaaccggg
      541 ttcataggtt ttggaaatga tcgggttaaa ctcggttgca ttgttataaa tagtgccgta
      601 attccaaaat tcattcaaat ccatacggcg cgatgtagca acggcaagca tctcgccgct
      661 ttgtggatcc ataatggcga taatgccatc ttgtgcaccg taagttccta aagcattatc
      721 aagaatttct tctgcggcag actgcaagtc acggttgatg gtaagaacca atgtagtgcc
      781 atcaggcgca ttgggaattt caaaggcttt gtttgggtcc gtcggcacca atacagtgac
      841 aggattgccc gctaataaac tatcgtattt ctcttctaca ccgaagtatc ccttgccttc
      901 acgattaaca aacccaagga tatttgaagc cagtgagttt tcgggataac tacgttgagg
      961 gtgagattta aactgcaagc ccgtcaaccc gcccattgaa ccttcagggg cttgttcgtt
     1021 caatgctttt ttgaggtctt gcaaataatt cgcttgtgaa gactcaacat aatctgcaag
     1081 gacaatgtat gagagcccat ctggtgggtt ttgaatgacc gacatcaaat tgtcgtaatt
     1141 cataccaaga gcatctgcta ctgcaaatgc aatggcgtga gcatccgtca taatgtttaa
     1201 gtctacgcca atttcataaa cgatcttctc cccagccaac aagcgaccgt tacggtcata
     1261 gatctcgccg cggttggggt aaaacgtgcg ccattcgtat gaataatctt tagaaagctg
     1321 gcgaaaggta tcggcttcgg ggctgttttg aatgcgcgtc atctgaaaca cgatggcaaa
     1381 cgcaaagaaa gccaatgcgg cagctaagac ttgactgcgg cgcccgtatt gctgtctcat
     1441 tgatatctcc gtgccaccaa gcgttcatcc atccaatcca aaagtgactg attgtattca
     1501 ggcggaatgg caagcacatt caattgaagt tgagcagagg aggaaagatt gactgcgtct
     1561 ttagcaacat agcccggcac cacaagatat tccagttcaa caggttccac aggtcggaaa
     1621 cccatttctt ttgcacgttg ttgcatgact tcagcagaag tcaggtttgc cagttgggtc
     1681 tgaagatcgc tgcttacctg ttggctgaca gcaatagaat cggtcaggtc ttgaatttca
     1741 cgaccagcaa tagcggtgcg agacgttaca tttaaataaa gagcggcaac catggcaagg
     1801 ctcaccacaa ccaataacgc attgccgatc cattggcgtt gcacgcgcca aggagcgacc
     1861 cggtaggcat ggatcacgtg
//
LOCUS       DAOQJE010000775         1879 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_51942_length_1879_cov_5.410636, whole genome shotgun sequence.
ACCESSION   DAOQJE010000775 DAOQJE010000000
VERSION     DAOQJE010000775.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1879)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1879)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1879
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_51942_length_1879_cov_5.410636"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..212
                     /locus_tag="PLT08_16395"
     CDS             <1..212
                     /locus_tag="PLT08_16395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE06108.1"
                     /translation="SKANYILFDCGDTGKTGKQVLEYAETKGIILRGESKKYGSDGWF
                     RVTIGSKEENELFVNTVLEFFGMKK"
     gene            273..1031
                     /locus_tag="PLT08_16400"
     CDS             273..1031
                     /locus_tag="PLT08_16400"
                     /inference="COORDINATES: protein motif:HMM:NF024811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HNE06109.1"
                     /translation="MSKIKAVFFDQDGVIIDTERDGHRVSFNMTFKEFGFTDEWDIEY
                     YHELLQVGGGKERMKKHWQNKGFSKPMTEEEIDALVKDMHKRKTALFVELIETGKLPL
                     RPGIHRFMKEAMDAGLKLAICTTSNEQAAKAITEGPLKDIKFDVVLAGDVVEKKKPDP
                     AIYNLALEKLGLKPDEVMVVEDSKNGLAAAKGAGAKVVVTTNHYTEKEDVSAGDVIVS
                     CLGDPDGEKAEMRKGDIKGFDGIVHVKQLMELFG"
     gene            1277..>1879
                     /locus_tag="PLT08_16405"
     CDS             1277..>1879
                     /locus_tag="PLT08_16405"
                     /inference="COORDINATES: protein motif:HMM:NF024910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HNE06110.1"
                     /translation="MAEKSQAHDKQVLHKLGYAQELSRRMSGFSNFAISFSIICILAG
                     GISAFPAAFNALGSGGAFLIWLVGGVLAMSVAVGMGQIASSFPTAGGLYHWSSHLGGK
                     FWGWATAWFNLIGLICVVSSVDVLLYTVFFRDLLLSTVLHVDVSTWGTMHQTIFLIIA
                     LGSQAILNHYYIDITTKLTDWSGYVILGLTVVLIITLFAFS"
BASE COUNT          494 a          453 c          471 g          461 t
ORIGIN      
        1 agtccaaagc caattacatc ttgttcgact gcggcgacac cggcaagact ggcaagcagg
       61 tgctcgaata tgccgaaacc aagggcatca tcctgcgtgg cgaaagcaag aagtacggct
      121 ccgacggttg gttccgcgtg accattggtt ccaaggaaga gaatgaactc ttcgtcaaca
      181 ccgtgcttga attctttgga atgaagaaat agttattcaa caaggcatga caggtttttg
      241 aaacctgtca tgcctattga aaagaggaac tcatgtccaa aattaaagca gtcttcttcg
      301 accaagacgg tgtcatcatc gacaccgagc gcgacggtca ccgcgtgtct ttcaacatga
      361 ccttcaaaga attcggcttc accgatgaat gggatatcga gtactaccac gagctcctcc
      421 aagtcggcgg cggcaaggaa cgcatgaaga agcattggca aaacaagggc ttctccaagc
      481 caatgaccga agaagagatc gatgcattgg taaaagatat gcacaaacgc aagacggctc
      541 tgttcgtgga actgatcgag acgggcaaac tgccccttcg cccgggcatt catcggttca
      601 tgaaagaggc gatggatgca ggtctaaaac tcgccatctg taccacatcg aatgaacaag
      661 cagccaaggc gatcaccgaa ggtccgctca aagatatcaa gttcgatgtt gtgttggctg
      721 gtgatgtggt tgaaaagaag aaacccgacc cagcgattta caaccttgcg cttgagaaac
      781 ttgggctcaa gccagatgaa gtgatggttg tggaagactc gaagaatggt cttgccgcgg
      841 caaaaggcgc tggtgcgaaa gttgttgtga ccaccaatca ctacaccgaa aaggaagatg
      901 ttagcgcagg agatgtgatc gtatcctgcc taggcgaccc agacggtgaa aaagctgaga
      961 tgcgcaaagg cgatatcaaa ggctttgacg gtatcgtcca cgtcaaacaa ctgatggagc
     1021 tatttggtta acccacaagg gtgcatggta atacgccatg cacccttgtg cttttatgga
     1081 caaacctggc gagaatgatt gttaacataa attattagtc gtacctttta ttaacagcca
     1141 gtgttttcaa catataattt cagaggagaa ctcccgcggg aggaggaggt gtcaatcacg
     1201 tgaataacaa tgtgctgctc gtaagtttgc tttcttgatc tacaaaacca actcatagtc
     1261 aaggagaatt acgaaaatgg ctgaaaaaag tcaagcgcac gacaagcaag tcctgcataa
     1321 attggggtat gcgcaggaac tgtcccgccg catgagcggg ttctcgaact tcgcaatttc
     1381 attttctatc atttgtattt tggctggtgg tatctcagca ttccctgctg cattcaacgc
     1441 gcttggatcc ggcggtgcat ttttgatctg gctggtcggt ggtgtgctcg ccatgagcgt
     1501 agccgttggt atgggacaga tcgcctcctc cttccccact gccggtggtc tctatcactg
     1561 gagctcacac ctcggcggga aattctgggg ctgggcaact gcctggttta acctcattgg
     1621 tttgatctgt gtggtttcct ctgtagatgt gttgctctac accgtcttct tcagagactt
     1681 gttgcttagc acagtcctcc acgtggatgt ctccacctgg ggcaccatgc accaaacgat
     1741 cttccttatt atcgccttgg gatcccaagc tattctgaac cattactaca ttgatatcac
     1801 aaccaaactc accgactgga gcggttacgt catccttggc ttgaccgtgg ttttgattat
     1861 caccctcttc gccttcagc
//
LOCUS       DAOQJE010000776         1879 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_51959_length_1879_cov_3.142544, whole genome shotgun sequence.
ACCESSION   DAOQJE010000776 DAOQJE010000000
VERSION     DAOQJE010000776.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1879)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1879)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1879
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_51959_length_1879_cov_3.142544"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            143..364
                     /locus_tag="PLT08_16410"
     CDS             143..364
                     /locus_tag="PLT08_16410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE06111.1"
                     /translation="MSETIIINLTKTVVAEMTKSYLELQYPEYSFKVVTYYSELKHVV
                     THQPIEGEQLQQMQAYAQGVSDVLSKLLA"
     gene            373..>1879
                     /locus_tag="PLT08_16415"
     CDS             373..>1879
                     /locus_tag="PLT08_16415"
                     /inference="COORDINATES: protein motif:HMM:NF022525.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2779 domain-containing protein"
                     /protein_id="HNE06112.1"
                     /translation="MPTRPQLSISKTDYKVARSCAAKLYYRMNKYPSSKDGDEYIELL
                     AEGGYMIGAIASLLFEDAVLVDEPDHDQAVALTQEYFKHENIILLEAAFESQRKFARP
                     DILIKQGDTLTLIEVKAKSFDSSVENPIRGKKGGIQSGWLPYLEDITFQALILRELFP
                     HAMLTCELMMPDKSKTTSIDNLHSLFALNQIAEDDSGYPRARFTFTGDVQELRRSHFL
                     TRINVDREVEELLPEVAAVAEVFIKSLNPLTKLPAPINFACKDCEYRVDKSEIKNGFR
                     ECWGELANPEPHILDLYYGSQVKEVQALIEAGKTSLYDLPESALSKKDGSLGPRAQRQ
                     LMQMRNSKSQTEWIDPALGDILSSHSYPLHFIDFETSGLAVPYHAGMRPFENVAFQWS
                     CHTIAKPGVEPIHTEWINLEDAYPNFAFAESLTNHLGRGGTVYMWAKHENSILKQIRA
                     QIDGRMEYAQLQDWLDWITDGRLVDMNDLTLKHYFHPVMKGRTSIKKVLDAV"
BASE COUNT          519 a          515 c          438 g          407 t
ORIGIN      
        1 aagagggtct gattactcaa gatgagtttg acgcaaaaaa agcagaaatc ttatctggta
       61 tataagaaga acttctggta aataaatatc ttttttttgt ataatgaaag aacagagtaa
      121 ttagggacat tccaatattc caatgagcga aaccattatc attaacctta caaaaactgt
      181 tgtagctgag atgacaaaat cctatttgga acttcaatac ccagaatact cttttaaagt
      241 agtcacttac tacagtgaac tcaaacacgt agttacccat cagcctatag aaggagagca
      301 actgcaacaa atgcaggcat atgctcaagg ggtttctgac gtattaagta agctattggc
      361 ataaatagac ccatgcccac ccgtccgcaa ctttccatct ctaagaccga ttacaaagtc
      421 gcgcgctcct gcgccgccaa gctgtactac cgcatgaaca aatacccgtc cagcaaagac
      481 ggggatgaat acatcgaact gctcgccgaa ggcggataca tgatcggcgc gatcgccagt
      541 ctgctctttg aggatgcagt cctggtggac gaacccgacc acgaccaagc cgtggcgctc
      601 acccaggaat atttcaagca cgagaacatc atcctgctcg aagccgcttt cgagagccag
      661 agaaaattcg cacgcccaga tatcctcatc aagcaaggcg ataccctaac cctgatcgag
      721 gtcaaagcca aatcgttcga ctccagtgta gagaatccca ttcgtggcaa aaaaggtggc
      781 atccaatcag gttggctgcc ctacctcgaa gacatcacct tccaagcgct catcctgcgc
      841 gagctcttcc cgcacgccat gctcacttgc gagctgatga tgccagacaa aagcaagacc
      901 acctcaatcg acaatctgca ctcactcttc gccctcaacc agatcgccga ggatgacagc
      961 gggtatccgc gtgcccgctt caccttcacc ggggatgtgc aagaactgcg ccgcagtcac
     1021 ttcctcaccc gcatcaatgt ggaccgtgaa gttgaggagc ttcttcccga agttgccgcc
     1081 gtcgctgagg tctttatcaa gagcctcaac ccactcacca aactacctgc accgatcaac
     1141 ttcgcctgca aggactgtga gtaccgcgta gacaaaagcg aaatcaagaa tggtttcaga
     1201 gaatgctggg gtgagctggc aaatcctgag ccgcacatcc tggatttgta ttacggtagt
     1261 caggtaaaag aggtacaggc actgattgag gcaggcaaga ccagcttgta tgacctgccc
     1321 gaatccgctc tcagcaagaa agacggctca ctgggtccac gtgcacagcg tcagttaatg
     1381 cagatgcgga attccaagtc tcagaccgaa tggatcgatc ccgccctggg cgacatcctc
     1441 tcttcgcatt cctatccgct gcacttcatc gacttcgaga cctctggctt ggcagtcccc
     1501 taccatgccg gtatgcgtcc gttcgagaat gtggcttttc agtggagttg ccacacaatc
     1561 gcaaaacccg gtgttgaacc aatccacacc gagtggatca acctcgaaga tgcctacccc
     1621 aacttcgcct ttgccgagtc gttgacgaat catcttgggc gcggcggcac agtctacatg
     1681 tgggcaaagc atgagaacag catcctcaag cagatccgtg cccagataga cgggcgtatg
     1741 gaatatgcac aactgcaaga ttggctggat tggatcacgg acggtcgcct ggtggatatg
     1801 aacgacttaa ccctcaaaca ttacttccac ccggtgatga agggcaggac gtcgattaag
     1861 aaggtgttgg atgcggtct
//
LOCUS       DAOQJE010000777         1875 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_52153_length_1875_cov_4.758791, whole genome shotgun sequence.
ACCESSION   DAOQJE010000777 DAOQJE010000000
VERSION     DAOQJE010000777.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1875)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1875)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1875
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_52153_length_1875_cov_4.758791"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1552)
                     /locus_tag="PLT08_16420"
     CDS             complement(<1..1552)
                     /locus_tag="PLT08_16420"
                     /inference="COORDINATES: protein motif:HMM:NF013126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 N-terminal
                     domain-containing protein"
                     /protein_id="HNE06113.1"
                     /translation="MDLEHQIGQKLLLAFRGKESLSPEIIQAFENYHPSGVTLFRPFN
                     IESPAQIKKLNRSLQDLARELGLPLLLIATDQEGGQLMAFGDGTPLPGNMALGATRSP
                     ELARKAGEVLGRELAAIGVNVNYAPSADVNINPNNPVVGVRSFGEDPALVGELAAAMI
                     EGIQSQGVAATVKHFPGHGDTASDSHHGLGTVPHSRERLHAVELTPFRSALQADAKLV
                     MTAHLGILSIDGKDAPPATLSPNILTGLLRDELDFKGVIITDAMDMHAIRQGELLRED
                     SLRAAQAGADILLMTTDPQDQARAFEALLQGAKSGELSQEKLQASLGRISELKKWLME
                     NATSPELGVIQCAEHMLVANEIAEKSITLVRDEKNYLPLKLEAGKRIAVIIPVPQDLT
                     PADTSSYIQPMLAESLRKYHSLTDEFKIPYAPSADEATKVLEQVREYDVIVMGTINAF
                     AEEKQAEFVRQLLKLDKPVINIALRLPYDLAAFPQASTFVCTYSILEPSMRAVAKAVF
                     GHSEMTGRL"
     gene            complement(1554..>1875)
                     /locus_tag="PLT08_16425"
     CDS             complement(1554..>1875)
                     /locus_tag="PLT08_16425"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HNE06114.1"
                     /translation="FVDGANHIQIWWEIILPLVKAPMATVATFLFVGLWNSLLTPLMY
                     LQTVDLYTLPVYVSTLYNINQTVQPWPTIMTAAVLTTAPLIFVFFFAQRFILESVVVS
                     GLKG"
BASE COUNT          486 a          475 c          477 g          437 t
ORIGIN      
        1 gcaaacgacc cgtcatctcg ctgtgaccga atacggcttt agccaccgcc cgcatcgacg
       61 gttccaaaat ggaataggta cacacgaagg tcgaagcctg cggaaatgca gcaaggtcat
      121 acggcaggcg caaggcaata ttgataacgg gtttatctaa cttaagcaac tggcgcacga
      181 actcagcttg cttttcttcg gcaaaggcat tgatcgtacc catcacaatc acatcatact
      241 ccctcacctg ctccaatacc ttagtcgcct catctgcact tggcgcatac gggattttga
      301 attcatcggt cagagagtga tatttacgaa gcgactcagc aagcatgggt tgaatgtaag
      361 aagaagtatc tgccggggtc aagtcctgtg gaacagggat gatgacggca atcctttttc
      421 cagcttcgag tttgagaggc agatagttct tctcgtcacg gaccagggta atggacttct
      481 cagcaatctc attcgccact agcatatgtt cagcacattg aataactccc aattcagggg
      541 aagtggcatt ctccatcagc cattttttca actctgaaat tcgtcctaac gaagcttgta
      601 atttttcttg cgagagctct ccacttttcg caccttgcaa taaggcttca aatgctcgcg
      661 cttggtcttg cgggtctgtg gtcatgagca aaatatccgc accagcttgg gcagcacgca
      721 aggagtcttc gcgcagtaac tctccctgac ggatagcgtg catgtccatc gcatccgtga
      781 tgatgacacc tttgaaatcg agttcatcgc gcaataagcc tgtgaggata ttgggagaaa
      841 gggtggcggg aggcgcatct ttaccatcga tggagagaat gccgaggtga gcggtcatca
      901 ccagtttagc atccgcttgc agcgcggagc ggaacggcgt caactctaca gcgtggagtc
      961 gctcacgcga gtgcggcaca gttccaagtc catgatgtga atcgctggcg gtatctccgt
     1021 gaccaggaaa atgcttgacc gttgccgcca ctccttgaga ttgaatccct tcgatcatgg
     1081 cagccgccag ttcccccaca agagctgggt cttcgccaaa cgaacgtacg cccacaacgg
     1141 gattgttcgg gttgatgtta acatcggcac taggtgcgta attaacattg acaccaatcg
     1201 ccgctaactc acgcccaagc acttcgcctg ctttccttgc aagttcgggt gagcgtgttg
     1261 caccgagagc catgttgccg ggcaaggggg taccatcgcc gaacgccatg agttgaccac
     1321 cctcttggtc agtggcaatc aacaaaagcg gcagacccaa ctcacgcgcg aggtcttgca
     1381 gcgatcggtt tagctttttg atctgcgcgg gcgactctat attaaatggg cgaaagagcg
     1441 tcacaccgct aggatgataa ttttcaaatg cctgaataat ctcgggcgaa agactttcct
     1501 tgccgcgaaa cgccaacaaa agtttttgac cgatctgatg ttcgagatcc acgttaacct
     1561 ttcaagccag aaacgacgac actctcgaga atgaatcgtt gagcgaaaaa gaatacgaaa
     1621 ataagtggag ccgtggtcag cacggcggcg gtcatgatgg tgggccaagg ctgaactgtt
     1681 tggttgatgt tgtacagagt ggaaacatag acaggcagtg tgtaaagatc aacagtctgc
     1741 aagtacatga gtggtgtaag caggctattc cacaaaccta caaagagaaa ggtggcaaca
     1801 gttgccatcg gtgctttgac taagggcaaa atgatctccc accaaatttg aatatgattc
     1861 gccccatcta cgaac
//
LOCUS       DAOQJE010000778         1872 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_52312_length_1872_cov_5.299395, whole genome shotgun sequence.
ACCESSION   DAOQJE010000778 DAOQJE010000000
VERSION     DAOQJE010000778.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1872)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1872)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1872
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_52312_length_1872_cov_5.299395"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..196)
                     /locus_tag="PLT08_16430"
     CDS             complement(<1..196)
                     /locus_tag="PLT08_16430"
                     /inference="COORDINATES: protein motif:HMM:NF036989.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribbon-helix-helix protein, CopG family"
                     /protein_id="HNE06115.1"
                     /translation="MTETEKITINLGAVDLGKIDLLVEQGHYSNRTDFIRSAIRSQLD
                     KHVLEVQQAVTRYSFGVGAFF"
     gene            complement(481..1563)
                     /locus_tag="PLT08_16435"
     CDS             complement(481..1563)
                     /locus_tag="PLT08_16435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE06116.1"
                     /translation="MFLLLTMFVFIAACSLPGTSAETTETTEETPTEVAAEAVESAPP
                     TEVSPVIQHTDVPVGLPEKQSGQAADFNASKILESKAVIGGDRFTFEKFERPFNASTM
                     DVYYSEIDIVNTLVYQDATWIYGIITLNQLQGNGLENVKYAIELDTDIDGKGDWLIIA
                     SKPSSTEWVVEGVQVFRDANKDVGGEQPMLTDNNPVAGDGFETIIFDQGNGSDPDIAW
                     VRISPNDPNTIELAFKQTTLESPKRYLINMWAGTKLIDPALFDISDNFTHEQAGAADT
                     GLNIFYPIKEVAEIDNSCRMAIGFKPTGKEDGLCESFVPGSPEQVLQPGGPGCSATQS
                     DIDACNATAAMSWHAASCSCVSDPPK"
BASE COUNT          486 a          383 c          399 g          604 t
ORIGIN      
        1 gaaagaatgc accgacacca aatgaatagc gggtgactgc ttgctgtacc tcaagtacgt
       61 gcttatctag ttgtgaccga atcgccgagc ggatgaagtc tgtgcggttc gagtagtgtc
      121 cttgttcgac cagcagatca atcttgccta aatctacggc acctaagttt attgtaattt
      181 tctctgtttc cgtcatttct ctcctttacc atctgtgtgg atggtatttt tcgtatttta
      241 ttgtgttttt ttggaattgt caagtttttt acatccaaat ggatgttata tggatgttta
      301 tttcgttttc tggcaacatt ctttactaac atgcatcacg tgacgatgaa taaataattt
      361 cgtaagagtt ttttatttgc gaaagacctt atccatattg aaataatttt tggatcaatt
      421 tccataaaaa aagtcccctg tctatagtaa cgggggactt taacctgaat tttatgtgaa
      481 ttatttggga ggatcagata cacaggaaca gcttgctgca tgccatgaca tggctgcagt
      541 tgcattgcat gcatcaatat cagattgagt ggctgaacat ccaggtccac caggttgaag
      601 cacttgttct gggctaccag gaacaaagga ttcacataga ccatcttctt tcccagttgg
      661 cttgaagcca attgccatac ggcaagagtt gtctatttca gccacttctt tgatagggta
      721 gaagatgttc agcccggtat ctgcagcgcc tgcctgttca tgggtgaaat tatcgctgat
      781 gtcgaagaga gcgggatcga tcagttttgt gccagcccac atgttgatga gatacctttt
      841 cggggattct aatgtagtct gtttgaaagc gagctcgatc gtattgggat cattaggaga
      901 gatgcgtacc caggcgatgt ctggatcgct tccattaccc tggtcaaaaa ttatggtttc
      961 gaatccatcg cctgctactg gattgttatc agtcagcatg ggttgctcgc cgccgacatc
     1021 tttgttggca tctctaaaga cctgtacacc ttctacaacc cattcagtgg aggagggttt
     1081 gcttgcaata attaaccaat cccctttgcc atcaatatct gtatccaatt caatcgcata
     1141 cttaacattt tccagcccat tcccttgaag ctgattaagg gtgatgatgc cataaatcca
     1201 ggtggcatcc tgatagacca aggtgttcac aatatcgatc tcagaataat agacatccat
     1261 ggtgctagca ttaaaaggac gctcaaattt ctcaaatgta aagcggtccc ctccaataac
     1321 ggccttggac tccaggattt tggaggcatt aaaatcagcg gcttgaccac tttgtttttc
     1381 aggcagaccg actggaacat cggtgtgttg gataacaggt gatacctcag taggtggggc
     1441 agattcaacc gcttctgcag caacttctgt tggagtttct tctgttgtct cggttgtttc
     1501 tgcagaagtg ccgggcaagc tacatgctgc gatgaagaca aacatcgtaa gcaataaaaa
     1561 caagactcta aacttactgg aattcattca tttctcctgt tgaattagta tgagttgatt
     1621 ttaatgacta acttgcagaa ttgaatggta cggcagtact tttttatgtg tattggtact
     1681 taagttctta agtatttatc actaatatga ctttagtact gtttgtaaaa ttttgattgt
     1741 aggagcttat aattctttta tgtctttcat taaacctcca acaaaaattc cactgtttct
     1801 gcgaataggt atttgggttt ctgaaaaagt cactggcaga cttatgctcc cacctcgtat
     1861 tctctcctgg ta
//
LOCUS       DAOQJE010000779         1872 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_52347_length_1872_cov_2.640066, whole genome shotgun sequence.
ACCESSION   DAOQJE010000779 DAOQJE010000000
VERSION     DAOQJE010000779.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1872)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1872)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1872
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_52347_length_1872_cov_2.640066"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..372
                     /locus_tag="PLT08_16440"
     CDS             <1..372
                     /locus_tag="PLT08_16440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE06117.1"
                     /translation="GYYSLLEMERTNAAVIAGRAGDRLTKSLQAIIEKYGLPFVAYNQ
                     GSIVHLETSGVMLLDLRNPIRLAKELKPRKHMIEEMGAAYMSQGLITLAGSRMYTSMA
                     DTDEVIDSALSKFETVLAACV"
     gene            521..1720
                     /locus_tag="PLT08_16445"
     CDS             521..1720
                     /locus_tag="PLT08_16445"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNE06118.1"
                     /translation="MTDKFKLYGYRWVVLGVFMFINLTIQMLWITYAPITGPAAAFYG
                     VTDLQIGLLAMSFMIAFIPLSIPVSWVIDTYGFRVAVGIGAVLMGIFGLLRGFAGANY
                     SLVLWSTVGIAAAQPFLLNAWTKVPANWFAIEERATAVGLVTLANLVGTALGMVLTPI
                     LTETMSIPTVQLIYGGIAAFSSVLFIIFAKETPPTPPGPDGSEVRALMLDGLKHAFTV
                     KSFWLYLFVSFVGLGIFNGVTTWVENIIRPRGFTPTDAGTLGALMLVGGVLGAVIIPP
                     FSDKTRKRQPYILAGMLLAIPGLVGLAFATTGWLLFVSAFAMGFFLVSTSPIGMQYAS
                     EVTQPTPEGTSNGLIQLFGQASVVFVYIMEALKTADGSFTPALLLAIGLLVVSAALVT
                     QMKDPQR"
BASE COUNT          386 a          444 c          530 g          512 t
ORIGIN      
        1 ggttattact ctttgttgga aatggaacgc acgaatgcgg cggtcattgc tgggcgggca
       61 ggggaccggc tcacgaaaag cttgcaagcc atcatcgaaa aatatggttt gccattcgtg
      121 gcttataacc agggctcgat cgttcatttg gagacttcgg gcgtgatgtt attggatttg
      181 cgtaacccga tccgcttggc gaaggaactc aagccgcgca agcatatgat cgaagagatg
      241 ggcgcggcgt atatgtcgca gggactgatc accttggctg gctcgcgcat gtatacatcc
      301 atggctgata cagatgaagt gatcgatagt gcattgagta agtttgaaac cgttttagcg
      361 gcgtgtgtgt gaatcacgtc accctgagcg acagcgacac atgcaccgcg ttgcggatgc
      421 agtgcggtga gggtctctac gccgatagta agagatcctt cggtcgcgaa gagcacgctc
      481 cctcaggatg acaataaaaa aaacaaagga gcatcatggc atgacagaca aattcaaact
      541 ttatgggtat cgctgggtgg tcttgggcgt gtttatgttc atcaacctga ccattcagat
      601 gttgtggatc acgtatgcgc cgatcacggg tcctgccgcc gccttctacg gcgtgacaga
      661 tttgcagatc ggcttgctgg cgatgtcgtt catgatcgct tttattccgc tttcgattcc
      721 tgtttcatgg gtgatcgaca cctatggctt ccgtgtggca gttggcatcg gtgcggtgtt
      781 gatgggcatc ttcggtctcc tgcgcggatt tgccggagcg aattactctc tcgttttgtg
      841 gagtactgtt ggcattgctg ccgcccagcc tttcctgctc aatgcgtgga cgaaagtccc
      901 tgcaaattgg tttgcgattg aagaacgtgc cacagcagtt gggcttgtca cgctggctaa
      961 tttggttggt acggcgctcg gcatggtgtt gactcctatt ctgaccgaaa ccatgtccat
     1021 cccgaccgtg caattgattt atggcgggat tgctgcattt tcatctgtct tattcatcat
     1081 ctttgcgaag gagactccgc ccacgcctcc tggaccagac ggcagcgaag tccgcgcttt
     1141 gatgttggat ggtttgaaac acgctttcac agtcaagtcg ttttggctgt atctgtttgt
     1201 ttcgtttgtg ggacttggca tcttcaatgg tgtgaccacc tgggttgaga atatcatccg
     1261 tccgcgcggg tttacgccga ccgatgcggg taccctcggt gcgttgatgt tggtgggcgg
     1321 cgtgttgggc gcagtcatca tcccgccgtt ttcggataag actcgcaaac gtcagccgta
     1381 tattttagct ggcatgttgt tggcgattcc gggtttagtt ggtttagcat ttgccacaac
     1441 cggttggcta ttattcgtct ctgcctttgc gatgggattc ttcctcgtca gcaccagtcc
     1501 gattgggatg caatacgcct ccgaagtgac ccagccgact ccggagggga cgtcaaatgg
     1561 cttgatacaa ttattcggtc aggcgtcggt ggtgtttgtg tacatcatgg aagccttgaa
     1621 gacggcagat ggctcattca caccggcatt gttgttggca atcggtttgt tggttgtgag
     1681 tgccgcgctt gtgactcaaa tgaaagaccc gcaacggtaa ctttttaatg tcaccctgag
     1741 cgttagcgaa tggtctctga gattaccgta gagatgctca ccgcactgca tccgcaccgc
     1801 ggtgcatgtg tcgctgtcgc tcagcatgac aaactataag cagagataaa caatgaccca
     1861 agaaaaactg at
//
LOCUS       DAOQJE010000780         1871 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_52371_length_1871_cov_7.245044, whole genome shotgun sequence.
ACCESSION   DAOQJE010000780 DAOQJE010000000
VERSION     DAOQJE010000780.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1871)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1871)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1871
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_52371_length_1871_cov_7.245044"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..826)
                     /gene="chvE"
                     /locus_tag="PLT08_16450"
     CDS             complement(<1..826)
                     /gene="chvE"
                     /locus_tag="PLT08_16450"
                     /inference="COORDINATES: protein motif:HMM:NF024799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HNE06119.1"
                     /translation="MEEHITYWRMVCLFQTGQATMLNDPGPVPHLFFTPIKTHPIKEK
                     KMSKKLLYNLLAVLVLLSLVLAGCGGGSSAGGDQKLVGISMPTKTSTRWISDGESMVK
                     SFEELGYATDLQFADNDIPNQIAQIENMVTKGADVLVIAAIDGSTLSDVLAKAKEAGV
                     LVIAYDRLISKTENVDYYATFDNFGVGVIQGTQLLNGLGLTPGEAAPAGVGPFNVELF
                     GGSPDDTNAFYFYDGAMSVIQPFIDSGTVVIPSGQMGMDVVGTLRWDGAVAQSRMEN"
     gene            complement(927..>1871)
                     /locus_tag="PLT08_16455"
     CDS             complement(927..>1871)
                     /locus_tag="PLT08_16455"
                     /inference="COORDINATES: protein motif:HMM:NF012742.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HNE06120.1"
                     /translation="YVSEGTRKRVENVVEKMGYRPSTLARSLIQKRSYTLGVVTFGLK
                     YIGPSRTLNGIADQADKLGYMLLMKELDNFDKHSLNEVMDSLLARQVDGIIWVAPEIG
                     DNHAFVDENADGGMPVPILFLAMRPRAEIDSVATDNYQGGVMATKHLLGIGRKRIGHI
                     SGPMTWWEAEERKRGWRDTLENAGLQVSEKFCAEGNWSSASGEQAFHQLLENYPDMDA
                     VFVGNDQMALSAMREAHRLGIRIPEQLAFIGFDNLPEAAYFYPSLTTVSQDLQLLGEE
                     SVQRIVEMIQARQNNQVLSAEARFIQPTLIVRESTQMV"
BASE COUNT          463 a          463 c          458 g          487 t
ORIGIN      
        1 ggttttccat gcgggattga gcaacagcgc cgtcccagcg aagggtaccg actacgtcca
       61 tgcccatttg accactgggg atcacaactg tgccggagtc gatgaagggc tggatgacag
      121 acatagcgcc atcatagaag tagaaggcat tggtgtcgtc aggggagcca ccgaagagtt
      181 caacgttgaa aggaccaacg ccagcaggag cagcttcacc gggggtgaga ccaagtccgt
      241 tgaggagctg ggttccctgg atcacgccga cgccgaagtt atcgaaggtc gcgtagtaat
      301 caacattttc ggtcttggag atcaagcggt cataagcgat cacgagcacg ccagcttcct
      361 tcgccttggc aagcacgtca gagagggttg aaccgtcaat ggcggcgatc acgagcacat
      421 cagcgccctt ggtgaccatg ttttcgatct gggcgatctg gttcgggatg tcattgtcag
      481 cgaactgcag atcggtggcg tagccgagtt cttcgaagga cttgaccatg ctttcgccgt
      541 cagagatcca gcgggtagag gtcttggtgg gcatggagat gccgaccaac ttctggtcgc
      601 cacctgcgct gctgccgccg ccgcagccag cgagcacaag gctgagcaga acgagaactg
      661 ctaacaggtt gtaaagcaat ttcttagaca tttttttctc cttgattgga tgtgttttta
      721 ttggcgtaaa aaataaatgg gggacaggtc ctgggtcgtt cagcatggtg gcttgtcctg
      781 tctggaagag gcacaccata cgccaatacg tgatgtgttc ttccatatgc tctaagtatg
      841 taactttctt gcaattgcac ctccttcttt tttccgtgct cttcgcgttc aacaaagaat
      901 ttcagaatag tgaaggacgg aaacgctcag accatctgcg tactttcacg gacgatcaga
      961 gttggttgaa taaaccttgc ttcagccgat aacacctgat tgttctgcct tgcttggatc
     1021 atttcaacaa ttctttgcac tgattcttca ccaagcaatt gcaaatcttg tgaaaccgtt
     1081 gttagagaag ggtaaaaata tgccgcttct ggtaagttgt cgaaaccgat aaaagccaat
     1141 tgttcaggga tgcgaattcc taaccgatga gcttcgcgca tggcacttaa tgccatttga
     1201 tcatttccaa caaagaccgc atccatgtcg ggatagttct ctagaagttg atgaaaggct
     1261 tgttcgccac ttgcagaaga ccaatttcct tctgcgcaaa acttttcaga aacttgcaaa
     1321 ccggcatttt caagggtgtc tcgccagccg cgctttcgtt cttctgcttc ccaccaagtc
     1381 atgggaccag aaatatggcc gatgcgtttt ctgccgattc ccagcagatg cttggtcgcc
     1441 atcacgccgc cttgataatt atcagttgca acgctgtcga tttctgcacg aggtctcatg
     1501 gctaagaaaa ggatgggaac tggcatgcca ccatcagcat tctcatctac aaaagcatgg
     1561 ttatcgccaa tttcaggtgc aacccaaata attccatcga cttgacgcgc gagcaacgag
     1621 tccatcactt cattgagact atgcttgtcg aaattatcaa gttctttcat caacagcata
     1681 taccctaatt tgtctgcctg gtctgcaatt ccattaagag tgcgagatgg accaatgtat
     1741 ttcaatccaa atgtaactac tcccaaggta tagcttcgtt tttggataag gctacgagca
     1801 agcgtactgg ggcggtagcc catcttttca actacatttt ccactcgttt tcgagtcccc
     1861 tctgaaacat a
//
LOCUS       DAOQJE010000781         1869 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_52496_length_1869_cov_3.081036, whole genome shotgun sequence.
ACCESSION   DAOQJE010000781 DAOQJE010000000
VERSION     DAOQJE010000781.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1869)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1869)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1869
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_52496_length_1869_cov_3.081036"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(88..1371)
                     /locus_tag="PLT08_16460"
     CDS             complement(88..1371)
                     /locus_tag="PLT08_16460"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNE06121.1"
                     /translation="MSKEHSRYRWFVVVVFFFFMLLHQTDKLMIGSLQVQISNDFNIN
                     NKQWGLVNSGALIVATILYPIWGYVYDRFSRTKLLSLASFVWGSTTWLNAIMKSFGGF
                     LVTRASTGIDDSSYPGLYTLIADYFGPTQRGKIFGILQLAQPIGYLLGMILALMVAPA
                     IGGWRSVFYITGSLGLVIALLIFFGVREMPRGKAEPEFENMTEMQTFKFSWAEAREIF
                     KKKTMWFVFLQGFAGVFPWNVITFFFFAYLEKERGYDANSILFTMAPVILILAAGYFV
                     GGALGDYAFKRSNKGRIIVSSIGVLLGAFFMYFAITTPLEAKNQFFIFMCLTAIFMPL
                     SSTNVTATVYDVTVPEVRSTAQAVEYFIENAGAAFAPIITGVIADMYDLKTAILLICT
                     VAWGLCFLFYLGAIFTIDKDANDLRAQMAERAKSM"
     gene            complement(1424..>1869)
                     /locus_tag="PLT08_16465"
     CDS             complement(1424..>1869)
                     /locus_tag="PLT08_16465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE06122.1"
                     /translation="RFFVIADVKKGRDTEVEWIQLTGVRKFIDRRATVESSEGVTEFL
                     KGKLPKPKEMSEAIIRLIVEYPREWDSLIDESALRKYAADAFEFHFVKRPQIESRVRI
                     DEGQVVSSLSPLDLLDQYFDSAKVSNADELKRLAQEIISEDSQDT"
BASE COUNT          557 a          461 c          411 g          440 t
ORIGIN      
        1 ctgacggttc aaggtgtatc gctattgatc ttgcgaaggt ctcataaata aaagcgggct
       61 tggcagtttg ccaagcccgc ttttatttca catagatttc gctcgttccg ccatttgagc
      121 gcggaggtca ttcgcgtctt tatcgatggt gaagatcgca ccgagataga acaggaagca
      181 caagccccat gccaccgtac agatcagaag gattgctgtc ttgagatcgt acatatctgc
      241 gatcacgccc gtaatgatgg gagcaaaagc cgcaccagca ttctcaataa agtattccac
      301 tgcctgagct gtagaacgca cttcggggac agtcacatca tatacggtcg ccgtcacatt
      361 cgtggaagaa agtggcataa agattgcagt aaggcacata aagataaaga actgattctt
      421 tgcctcaagc ggagtagtga tggcaaagta cataaaaaat gcacccaaca acacaccaat
      481 ggatgaaaca ataatacgac ctttattgga gcgcttaaaa gcgtagtcac ctaatgcgcc
      541 gccgacaaaa tatccagccg caaggatcaa gataacaggc gccatggtaa agagaatgct
      601 gtttgcatcg tatccgcgtt ctttttcgag ataggcaaag aagaagaacg tgatcacatt
      661 ccacgggaac acgcctgcaa agccctgtaa aaagacaaac cacatggttt tcttcttgaa
      721 gatttcccgg gcttctgccc acgagaattt gaaggtctgc atctcggtca tattttcaaa
      781 ttcaggctcg gctttaccac gcggcatttc acgcacgcca aagaagatca ataacgcgat
      841 caccaaccct aaagaaccgg taatatagaa aacagagcgc catccgccaa tggcaggagc
      901 aaccatcagt gcaaggatca tccctaataa ataaccaata ggttgagcca attgcaaaat
      961 gccgaagatc tttccacgct gggtaggacc aaaataatcg gcgatcaaag tgtacaagcc
     1021 cgggtacgaa gagtcatcga taccggttga ggcgcgtgta acaagaaagc cgccaaagct
     1081 tttcatgatg gcattcaacc atgtcgtaga accccaaaca aaagaagcca gtgaaagtaa
     1141 ttttgtgcga gagaagcgat cgtatacata cccccagatc gggtacaaga tcgttgcaac
     1201 gatcaacgct cctgaattga ccaaacccca ctgtttattg ttgatattga aatcattgct
     1261 gatctgcacc tgcaacgaac cgatcattaa cttatcggtc tggtgcaaca gcatgaaaaa
     1321 gaagaacaca accaccacaa accagcgata ccgtgaatgt tccttggaca taaaaacctc
     1381 atgacattgg gatgcccaag tatcagggtt cagaggatag gtgtcaagtg tcttgtgagt
     1441 cctctgagat gatttcttgt gccaaacgtt tcaattcatc ggcattcgaa actttcgccg
     1501 aatcaaaata ttggtcgagc aaatcaagcg gactgagact gctcaccacc tgcccttcgt
     1561 caatgcgcac acgcgactca atttgaggtc gctttacgaa gtgaaactcg aacgcatccg
     1621 ctgcatattt tcgcaacgct gattcgtcaa tcaatgaatc ccattctctt ggatactcaa
     1681 cgatcaagcg gatgattgct tcagacattt ctttgggttt gggcagtttg ccctttaaga
     1741 actcggttac gccctcactc gactcgacag ttgcccggcg gtcgataaac tttctcaccc
     1801 ctgtgagttg aatccactct acctcggtgt cgcgtccttt tttcacatct gcaatgacga
     1861 agaaacgtt
//
LOCUS       DAOQJE010000782         1867 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_52591_length_1867_cov_6.285320, whole genome shotgun sequence.
ACCESSION   DAOQJE010000782 DAOQJE010000000
VERSION     DAOQJE010000782.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1867)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1867)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1867
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_52591_length_1867_cov_6.285320"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..295)
                     /locus_tag="PLT08_16470"
     CDS             complement(<1..295)
                     /locus_tag="PLT08_16470"
                     /inference="COORDINATES: protein motif:HMM:NF016221.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016531 - copper chaperone activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper chaperone PCu(A)C"
                     /protein_id="HNE06123.1"
                     /translation="MKKIFAFLFASALLLSACGGGMAGGEEHTDGEGVEAHDYWVRAA
                     MAGGNSAAYMLLHNHGAEDDALIAVSSDLADAVEMHLSQMKADGTMEMIQQEKI"
     gene            complement(329..901)
                     /locus_tag="PLT08_16475"
     CDS             complement(329..901)
                     /locus_tag="PLT08_16475"
                     /inference="COORDINATES: protein motif:HMM:NF019826.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR family transcriptional regulator"
                     /protein_id="HNE06124.1"
                     /translation="MATNSLDREQKILEHLKTHKTASIQELAEAFGVSNMTIHRDLHK
                     LANAGQIIKKHGGAALPNSGVKALEEPSSCAMCGKPASQRTIFIVKFDNGEELRACCA
                     HCGLMMQSREKNVWQSLTVDFLYGHMISAGQAYYLVGSELNICCVPSILAFGSKADAE
                     KFQKGFGGQLLDMENTIHHMHGMMHMHSDM"
     gene            complement(981..>1867)
                     /locus_tag="PLT08_16480"
     CDS             complement(981..>1867)
                     /locus_tag="PLT08_16480"
                     /inference="COORDINATES: protein motif:HMM:NF024896.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNE06125.1"
                     /translation="RDSRLLPISGSDLPEGWAGKPHALYQASAVAKGEWLCFVDADTF
                     LAPNALSAVYAKASETKADLFTVMTKQILGSFWERTVMPLVMTALSVGFSPRKVNDPS
                     RRDAVANGQFIFIKRSIYDLIGGHEKIKDQIVEDKALSENVKWNGHRLVVANGMQLIS
                     TRMYTSLETMWEGWTKNIYLGLRDHPSLLLLGAFGATLALLAALFLPVWPLLGLGWFL
                     NSGGWMALVVIVEALFVWGYLIVIRARIAQEMDIPRWYAWTTPLGAGVFAAMMLTSAW
                     KVISGQGVTWRGRNYKPK"
BASE COUNT          487 a          510 c          397 g          473 t
ORIGIN      
        1 caatcttttc ctgctggatc atttccatgg tgccgtcggc tttcatttgg ctcaggtgca
       61 tttcaaccgc atctgccaaa tcagaactga cagcaatgag ggcatcatct tctgccccat
      121 ggttatgaag gagcatataa gcagcgctat tgccacctgc catcgctgcg cggacccaat
      181 aatcatgagc ttccacacct tccccatcgg tgtgttcttc cccacctgcc ataccacccc
      241 cgcatgcact taacagaagc gcactggcaa aaagaaaagc aaaaattttt ttcattctct
      301 tattcctcac aaattttatt tttctgaatc acatgtctga atgcatgtgc atcattccat
      361 gcatatggtg aatagtgttt tccatatcaa gcaattgccc gccaaaccct ttttgaaact
      421 tctctgcatc tgcctttgag ccgaatgcaa gaatacttgg cacgcagcaa atatttagct
      481 cgctcccaac aaggtaataa gcctgcccag cactgatcat atgaccatat aaaaaatcta
      541 ctgtcagaga ttgccaaacg ttcttttcgc ggctttgcat cattagaccg caatgagcac
      601 agcaagcacg cagttcttcc ccgttatcga atttgacgat gaagatcgtc ctttgagaag
      661 cgggtttccc gcacatggca catgaagatg gttcttctaa tgcttttacg cccgagttcg
      721 gtaaggcagc accgccatgc ttctttatta tctgacccgc atttgctaac ttatgcaaat
      781 cgcggtggat ggtcatattc gacacgccaa aagcctcagc cagttcttgg attgaggcag
      841 ttttatgtgt cttgagatgt tcaaggatct tttgttcgcg gtctaaggag ttggtagcca
      901 tatgttatat tttgttatct ttaacaagag attacgaaat ataacaactc aaccaatatt
      961 tatcaagatg acatttatca ctactttggc ttatagttcc gtccacgcca ggtaaccccc
     1021 tgccccgaaa tcactttcca tgccgaggtc aacatcatgg cggcaaacac ccctgcccca
     1081 agcggagtgg tccatgcata ccagcgcgga atgtccatct cttgcgcaat tctggcgcgg
     1141 atgacaatca aatagcccca cacaaacaat gcctcaacaa tcacaaccaa tgccatccac
     1201 ccaccagaat tgagaaacca gcccaagccc aacaacggcc acaccggaag gaataatgcg
     1261 gcgagcaatg ccaaggttgc accaaatgcg ccgagcaata acaacgatgg atggtctcgc
     1321 aacccaaggt agatgttctt cgtccagcct tcccacatgg tttccaaaga cgtgtacatg
     1381 cgagtgctaa tcagttgcat gccatttgcc accaccaagc ggtgaccatt ccactttaca
     1441 ttttcagaga gcgctttatc ttccacaatt tgatctttaa ttttttcatg cccgccgatc
     1501 aaatcataga tactgcgctt gatgaagatg aactgtccgt tcgctaccgc atcacggcgc
     1561 gatgggtcat tgaccttgcg cggagaaaag cctactgaga gcgcggtcat caccaacggc
     1621 atcaccgtcc gctcccagaa ggaaccgagg atttgcttcg tcatcacggt gaacaaatcg
     1681 gcttttgttt cgctggcttt ggcatacacg gcggaaagcg cattcggcgc gaggaaggta
     1741 tcggcatcta caaagcaaag ccactcgcct ttcgccacag cagaggcttg gtacaaagca
     1801 tggggtttgc ccgcccagcc ttcgggtaag tctgagccgc tgattgggag gagacgagag
     1861 tcgcggg
//
LOCUS       DAOQJE010000783         1863 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_52818_length_1863_cov_7.419801, whole genome shotgun sequence.
ACCESSION   DAOQJE010000783 DAOQJE010000000
VERSION     DAOQJE010000783.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1863)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1863)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1863
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_52818_length_1863_cov_7.419801"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..265
                     /locus_tag="PLT08_16485"
     CDS             <1..265
                     /locus_tag="PLT08_16485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE06126.1"
                     /translation="VVKAFNTTYAGTLVAGEVAGQKLDVFIAGDDADAKAKVAQIVTD
                     GGMRAVDAGPLSRARQIEGMQLLHIVLQGTLGTNWGSALKIIA"
     gene            342..986
                     /locus_tag="PLT08_16490"
     CDS             342..986
                     /locus_tag="PLT08_16490"
                     /inference="COORDINATES: protein motif:HMM:NF014628.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LUD domain-containing protein"
                     /protein_id="HNE06127.1"
                     /translation="MVTKEMNINMAPNLAFAKLASEAQIQKVTKALEANGIKVLIAET
                     GEEAKNMVLGLVPQGAEVYSNMSKTLDTLGLSAEFDKSGRYNAVRPRVLALDRKTQAD
                     EIRKLRTIPDYIVGSVHAITEAGEVLTASGTGSQISSYAYGAGKVIWVVGTQKIVSDL
                     SEGFRRIKEYSHPLEDARMQEAYGVNSSLNKILVLTRDMPGRITIVLVKEELGF"
     gene            1067..>1863
                     /locus_tag="PLT08_16495"
     CDS             1067..>1863
                     /locus_tag="PLT08_16495"
                     /inference="COORDINATES: protein motif:HMM:NF013098.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HNE06128.1"
                     /translation="MSTQTNSPAKQEYSVHPATRMGHVSLTVANLENQIVFYEKAIGL
                     KLHWREGNKAGLGAGGADLLQLTEEPNLKRYRGGTGLYHFAIVFPNRRELAIAMARLF
                     ALKVPNSPTDHVMTKTTYLSDPEGNGIELYCESPEDGSWTIKDGNYEARWADGRWSDG
                     REALDVDALFTHLKEDDKLDAPVPAETRMGHVHLHVRDIPEAVDFYHGVLGFDVMGIS
                     KGIGMAFVSAGGYHHHIGLNTWNGRGAPPPPPDAVGLRFMTVDYPNPS"
BASE COUNT          484 a          480 c          484 g          415 t
ORIGIN      
        1 ggtcgtcaaa gccttcaaca ccacgtatgc tgggactctc gtggctggtg aagtcgccgg
       61 tcagaagctc gatgttttca tcgctggtga tgacgccgat gccaaagcca aggtcgctca
      121 gatcgtcacc gacggtggca tgcgcgccgt ggatgcaggt ccgctgagcc gcgcccgcca
      181 gatcgaaggc atgcaattgt tgcacattgt attgcaggga actctcggca ccaattgggg
      241 cagtgcgctt aagattattg cgtagtcttt tggtaaaagc tcatggcact tgcatttgaa
      301 ctatgcaagt gccatgcatg ctgtaagaaa agaaggttcg aatggttaca aaagaaatga
      361 atatcaacat ggctccaaac cttgcatttg ctaaattagc aagtgaggcg caaattcaaa
      421 aagtgacgaa agccttggaa gccaatggaa taaaagtact cattgccgaa acaggtgaag
      481 aagccaagaa catggttctg ggtctggttc cgcaaggcgc ggaagtgtat tccaatatgt
      541 cgaagacgct cgatacgctt ggcttgtctg ccgagttcga taaatctggg cgttataacg
      601 cggtccgccc gcgtgtgctg gcgctagacc gtaagacaca agcagacgaa atccgcaagc
      661 tacgcacgat tccagattat attgttggca gcgtccacgc gattacagaa gcaggtgaag
      721 tgcttacggc ttcaggcaca ggcagccaaa tttcctccta tgcctatggc gcagggaaag
      781 tgatttgggt ggtcggaaca caaaagattg ttagcgatct aagcgaaggc ttccgccgca
      841 tcaaagaata cagtcatccg cttgaagatg cccgcatgca agaagcctat ggcgtcaaca
      901 gttcgctcaa caagatatta gttctaactc gtgatatgcc agggcgcatc accattgttc
      961 tggtgaaaga agaacttggg ttttagcccg aaccaaagaa agtatttccg tgtttaagtc
     1021 atctagtaca taggcttaga atcatggacg aaagataaag gtatatatgt caactcaaac
     1081 aaactccccc gcaaagcaag aatactcggt tcaccccgcc accagaatgg gacacgtttc
     1141 cctcactgtt gccaaccttg agaatcagat cgtgttttac gaaaaagcca tcgggttgaa
     1201 actgcactgg cgcgaaggca ataaagccgg tctcggagcc ggaggcgcgg atctacttca
     1261 acttactgaa gaaccgaatc tcaaacgcta tcgtggcggc acgggcttgt atcactttgc
     1321 catcgtattc cccaatcgcc gcgagcttgc gattgccatg gcgcgtttgt ttgcgttgaa
     1381 agttcccaac tcacccaccg accatgtcat gaccaagacc acctacctca gtgaccctga
     1441 aggtaacggg atcgaactgt attgcgaatc gcccgaagat ggttcgtgga cgatcaaaga
     1501 cggcaactat gaagcccgct gggcagacgg acgctggagc gatggacgcg aagcactcga
     1561 cgtcgatgcg ctcttcactc atctcaaaga agatgacaag cttgatgccc ccgtccctgc
     1621 cgaaacccgc atgggacatg tccacttgca tgtgcgcgat attcccgaag ctgtcgattt
     1681 ttatcacggt gtgctcggct tcgatgtgat gggcatttcc aaaggaattg gcatggcatt
     1741 cgtctctgca ggcggatacc accaccatat cggtctcaac acctggaacg gacgcggcgc
     1801 gcctcctcct ccgcccgatg cggtgggact gcgcttcatg accgtggact acccgaatcc
     1861 ttc
//
LOCUS       DAOQJE010000784         1858 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_53087_length_1858_cov_5.516362, whole genome shotgun sequence.
ACCESSION   DAOQJE010000784 DAOQJE010000000
VERSION     DAOQJE010000784.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1858)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1858)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1858
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_53087_length_1858_cov_5.516362"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1813)
                     /locus_tag="PLT08_16500"
     CDS             complement(<1..1813)
                     /locus_tag="PLT08_16500"
                     /inference="COORDINATES: protein motif:HMM:NF013736.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HNE06129.1"
                     /translation="MHSNFEMLIPERFRDTHAKQKKAFLKTPQKRAMGSGLDIYGLHQ
                     SGKEIPVEVGLNPVTIDGEKYVLVSVSDISTRKEMEVALAASRLKESEEKFRTLVEQN
                     PAIVYISGLHQHIGVTYISPQIQTLGFTQAEWIADPNLWVEQIHPEDQKRVLKEVEEF
                     ALKNQPFKSEYRLLAKDGHVRWFLDEAVDIIDSNGSAIIRQGFMLDISARKDAEETLS
                     SREHYLALLNAITTSIIASQDMDGSIESLVEGLKGLLSADDCYMTRWDEEIEKPVSWG
                     STAEMEQPYMTMTFPDGLRSMSSSVIEEGHALVAEDVFNSPYVSPEIAKMFPAHSMLG
                     IPLLYRNKKLGAIIVAYNTLHKFDTGEIERAEQAGRQIAIAMWSVQQDIALKKRLKEQ
                     ETLAQITTTLSQVEHIGLSNVLDLIVHAARELIPEAQQAVIHLMDRGNAYLTPQAVSG
                     YEQTSESGGKMRVGEGAAGLAMSEGKSVYIPSIHTDERFIHLSSQVKYNSLLVTPIIS
                     SSQKLGTISIQSEKAFAFSKSAINLLNELGQQAAIAIENTRLYDAAQQELKERFLAEA
                     ALRSSEERYRSVSEDIPAMICRFDRGGTLTYVNQAYAD"
BASE COUNT          469 a          426 c          401 g          562 t
ORIGIN      
        1 aatctgcata ggcttggttg acataggtca gcgtaccacc tcggtcaaag cgacaaatca
       61 tggctgggat atcttcagaa acactgcgat aacgttcctc actgctgcgt aaggctgcct
      121 ctgctaggaa acgttctttt agttcctgtt gggctgcatc gtaaagcctg gtattttcaa
      181 tcgcgatggc ggcttgttgt ccaagttcgt taagcaagtt gatggcgctt ttagagaaag
      241 caaatgcctt ctcgctctgt atgctaatag tgccaagctt ttggctactg ctaataattg
      301 gcgtaactaa aagagagttg tatttcactt gcgagcttaa gtgaataaag cgctcatcag
      361 tatggatcga aggaatgtaa acgcttttgc cttcagacat ggcaagccct gcagcgcctt
      421 cacccacgcg catcttacct ccgctttcgc tagtttgttc atacccagaa acagcttgtg
      481 gggtaaggta ggcattgcct ctatccatga ggtgaatcac agcttgttgc gcttcaggaa
      541 ttaattccct tgcggcatga acgatcaagt caagcacgtt tgataaacca atatgctcca
      601 cctggctaag tgtggtggtg atctgggcaa gggtttcttg ctctttcaag cgtttcttaa
      661 gtgcaatatc ttgttgaaca ctccacatag caatagcaat ttgcctgcca gcttgctctg
      721 ctctttcaat ttcgcctgtg tcaaatttgt gcaaggtatt gtaggcaaca atgatcgcgc
      781 caagtttttt atttctgtat agaagtggaa ttccgagcat ggaatgtgcc gggaacattt
      841 ttgcaatttc cgggctgacg taaggagaat taaagacatc ttctgcgaca agtgcgtgac
      901 cttcttcaat gactgagctg ctcattgagc gtaatccgtc agggaaagtc attgtcatat
      961 acggctgttc catctctgcc gtagagcccc aggacacagg tttttcgatc tcttcatccc
     1021 aacgagtcat ataacaatcg tcagcgctga gaagtccttt taacccttca actagactct
     1081 caatggagcc atccatatct tgggatgcaa tgatcgaagt agtaatggca ttcaataaag
     1141 caagataatg ttcccgagaa gataaggtct cttcggcatc ttttctggcg ctaatatcca
     1201 gcataaagcc ctggcggata atggccgatc cgttactatc aatgatatct actgcttcat
     1261 ctaagaacca acgtacatgc ccgtcttttg ctagtagtct gtattctgac ttgaatggct
     1321 gattttttaa ggcaaactct tcgacttctt tcaacactct tttctgatct tcaggatgaa
     1381 tttgttctac ccacaaattt gggtcggcaa tccattctgc ttgagtgaaa cccaacgttt
     1441 gtatctgcgg actgatataa gtgaccccga tatgttggtg aagacccgaa atataaacaa
     1501 tagccgggtt ttgttctacg agtgtacgga atttttcttc agactctttt aatcggctgg
     1561 ctgccagggc aacttccatc tcttttcttg tagaaatatc agagacagaa accagaacat
     1621 acttttcgcc gtcaatagtg accggattaa gaccaacctc aactggaatt tccttgccgc
     1681 tttgatgcag accatagata tccaaccctg accccattgc cctcttttga ggagttttga
     1741 gaaaagcttt cttctgtttc gcgtgcgtat cccgaaacct ttcggggatt agcatttcaa
     1801 aattgctgtg tattaaatct ttgcgtgaat acccaaatag atcttctgca tgtgcgtt
//
LOCUS       DAOQJE010000785         1855 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_53250_length_1855_cov_6.014444, whole genome shotgun sequence.
ACCESSION   DAOQJE010000785 DAOQJE010000000
VERSION     DAOQJE010000785.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1855)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1855)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1855
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_53250_length_1855_cov_6.014444"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..298)
                     /locus_tag="PLT08_16505"
     CDS             complement(<1..298)
                     /locus_tag="PLT08_16505"
                     /inference="COORDINATES: protein motif:HMM:NF013921.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - transposition, DNA-mediated
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HNE06130.1"
                     /translation="MPYARKDLLTWQKGMYYHIYNRGISRSPIFREEGNYLFVLGKLK
                     AACRENQLSMIAYCLEPNHYHFFIRQDGDLPAGNLPQYVFNSYTKAYNKKYEHSG"
     gene            complement(357..>1855)
                     /locus_tag="PLT08_16510"
     CDS             complement(357..>1855)
                     /locus_tag="PLT08_16510"
                     /inference="COORDINATES: protein motif:HMM:NF014442.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006306 - DNA methylation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="N-6 DNA methylase"
                     /protein_id="HNE06131.1"
                     /translation="KDILLNNIYGVDIDPQAVEVTKLSLLLKVLEGESHETIGSQLAL
                     FQERVLPDLGRNIKCGNSLIGNDYFEGRQLTMLVDEEERYRVNAFDWKAEFPQVFIQG
                     GFDAVIGNPPYGAELTLQDNEYVRTRYEVINRDIDTYALFIELSLNLCKADGRISMIV
                     PTGWYSGARFSKLRKLFVCITNPEIIVNLPYDIFKAWVDTTIFVANKRDNRLEWPRKL
                     DSQTKLITFPKRYSIKSQEDYENKESDINIVDWFDNGTDEFLTYANTKVVGIIRKIEN
                     ASKPLSEFADVQRGVTPFNLEEAANYATSEPAFSGTVRRYSLERGDLTYIRFDSTLAE
                     YKPEKYFKGPRLLLRELISRQFRLQAVKATDDFVTNKSMQSILQLQNSPDLNYLLGML
                     NSRLTSWYFLSKSNVGQRDDFPKIVLKETRLLPIRPINFDDPADKARHDKLVSLVERM
                     LALHKQAPRTPQEQEMLKREIESTDKAIDSLVYELYGLSEEEIRIVEG"
BASE COUNT          465 a          406 c          391 g          593 t
ORIGIN      
        1 tgccgctgtg ctcgtatttt ttgttatagg ctttggtgta actgttgaag acgtattgag
       61 gcaaatttcc ggcggggaga tcgccatcct gccggatgaa gaagtgataa tgattgggct
      121 ccaggcaata ggcgatcatg ctgagttgat tttcgcggca ggctgctttc aatttgccga
      181 ggacaaagag gtagttgcct tcttctcgaa agatgggcga cctgctgatg ccgcgattgt
      241 agatgtgata gtacatccct ttttgccagg tcagtaaatc tttacgagca taaggcatgg
      301 tttcctcctt ggggggtcta aagtgacagt cacctttaag gtgactgtca cttggtttaa
      361 ccttccacga tccgtatctc ttcctcgctc aacccatata attcatagac caaagaatcg
      421 atcgctttgt cggttgactc gatctcgcgc ttgagcatct cctgctcttg gggagttcgg
      481 ggggcttgct tgtgcagtgc caacatgcgc tcgacgagcg agacgagttt gtcgtgccgc
      541 gctttgtcgg cggggtcgtc gaagttgatt gggcggatgg gaagtaaacg tgtctctttt
      601 agaacgattt ttggaaaatc atccctctgt cctacatttg atttgctcaa aaaataccac
      661 gatgttaagc ggctgttcaa cattcccaat aaataattaa ggtctggtga attttgtaac
      721 tgcaaaatgc tttgcattga tttattggta acaaaatcat ctgtagcttt cacggcttgc
      781 aatcgaaatt gcctactgat caattcgcgc aaaagcaatc ttggtccttt gaagtatttt
      841 tctggcttat attcagcaag tgtgctatca aaacgaatat aagttaaatc accacgctca
      901 agtgaatagc gtctgacagt tccactgaat gcaggttcac ttgtggcgta atttgccgct
      961 tcttcaagat taaatggtgt aactcccctt tgtacatctg caaattctga gagaggctta
     1021 cttgcatttt ctatttttct tattattcca acaactttgg tgttggcgta ggtcaaaaat
     1081 tcatcagtcc cgttatcaaa ccagtctaca atatttatgt cgctttcttt attttcataa
     1141 tcttcttgag atttgatact atacctcttc gggaaagtta ttagtttagt ttgactatct
     1201 agttttcttg gccattcaag cctattatca cgtttgttgg caacgaaaat agttgtgtct
     1261 acccatgctt taaaaatatc gtagggcaaa tttacgataa tttcagggtt agttatacat
     1321 acaaagagtt ttcttaactt actaaaccta gcaccactgt accatccagt tggaacaatc
     1381 attgaaattc ttccgtctgc tttacataaa tttaaagata gttcaataaa caaagcgtat
     1441 gtatcaatat ctctgtttat aacttcatat ctagttctta catattcatt gtcttgaaga
     1501 gttagttctg caccatacgg cggatttcca atcacggcat cgaagccgcc ttgaatgaag
     1561 acctgcggaa actcggcttt ccagtcgaag gcgttcacac ggtaacgttc ttcttcatct
     1621 accaacatcg tcaattgtcg cccctcaaaa taatcgtttc cgatcaacga gttaccgcat
     1681 ttgatgttcc tgcccaaatc tggcagcact cgctcttgaa acaacgccaa ctgcgagccg
     1741 atggtctcat ggctttcacc ttccagcacc ttcagcagga gcgagagttt ggtcacttct
     1801 acggcttgcg ggtcaatgtc cacgccgtaa atgttgttaa gcaggatgtc tttct
//
LOCUS       DAOQJE010000786         1848 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_53664_length_1848_cov_3.984384, whole genome shotgun sequence.
ACCESSION   DAOQJE010000786 DAOQJE010000000
VERSION     DAOQJE010000786.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1848)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1848)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1848
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_53664_length_1848_cov_3.984384"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..997
                     /locus_tag="PLT08_16515"
     CDS             <1..997
                     /locus_tag="PLT08_16515"
                     /inference="COORDINATES: protein motif:HMM:NF013094.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ThiF family adenylyltransferase"
                     /protein_id="HNE06132.1"
                     /translation="VTYNDALVPELNYKEELSRTVSAWGKKEQQNLMRLRVGIIGAGN
                     VGSIVAETLARMGVAHIKLIDFDSVEQHNLDRILHSRPLGALLQHAKVQMLSRELKNS
                     ASASPFTVDALEYSIVEDYGFRAALDCDILFSCVDRPLARFVLNFIAYAHLIPVIDGG
                     IRAEAKKNQNGMLRADWRSHIAGPSHICLECIGQYRSEDVSTERSGYLDDPSYIAGLP
                     DSHFIKHNENVFAFGLSLAALEIQQFLALLLNLPGELVRKSQMYHFVDDTTEYDERTC
                     NSNCSFPPLTALGDHAETKVTSQHLIAEKARANRSRFHHSLSYWILKVFKKKCNQ"
     gene            complement(1481..>1848)
                     /locus_tag="PLT08_16520"
     CDS             complement(1481..>1848)
                     /locus_tag="PLT08_16520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE06133.1"
                     /translation="WELRYQEYEAIEGCLPGPNGRGNYNCGGGTGHRGITGYEWRNRS
                     SGGEIPPSAVANLPAGRMADINNDGTPDAYWDTNLTLRRIDDAGSISNPQYQRSWNWG
                     GIIYWAVREGQGQIGWPEK"
BASE COUNT          526 a          380 c          394 g          548 t
ORIGIN      
        1 agttacctat aacgatgcgc ttgttcctga attgaattat aaagaggaac tctctcgcac
       61 tgtatctgct tggggtaaaa aagaacaaca aaacctcatg cggttgcgcg tgggaattat
      121 cggcgcaggt aatgtcggaa gtattgttgc tgaaacactt gctcgaatgg gcgttgcaca
      181 tattaagttg attgatttcg actctgttga gcaacataac ttagacagaa ttttacattc
      241 gcgccctttg ggtgccttgt tgcaacatgc caaagtccaa atgttatcgc gagaattaaa
      301 gaacagtgca agtgcatccc cgtttacagt agatgcactt gaatatagca ttgtggaaga
      361 ttatggtttt cgcgctgcgc ttgattgcga tattctcttt agttgtgtag atcgaccatt
      421 agccagattt gttctgaact ttattgcata tgctcacttg attcctgtca ttgatggtgg
      481 catccgagct gaggcaaaga aaaaccaaaa tggaatgctc agagcagatt ggcgatcaca
      541 tattgctgga ccttctcata tctgtttgga gtgcataggt caatacaggt cagaagatgt
      601 ttcaactgaa agaagtgggt atttggatga ccctagctat attgcagggt tgccagatag
      661 tcactttatt aaacataacg aaaatgtttt tgcttttgga ctaagcttgg cagctcttga
      721 gattcagcaa tttcttgctt tgttgttaaa cctccctggt gaattagttc ggaaatcgca
      781 gatgtatcat ttcgtggatg atacaacaga atatgatgaa cgaacctgca attctaattg
      841 ctcatttcct cctttgacag cattaggtga tcatgcagaa actaaagtta catcacagca
      901 ccttatagca gaaaaggcac gagctaaccg atctcgtttt catcactccc tgagttattg
      961 gatattaaag gttttcaaaa agaaatgcaa tcagtgatgc tattgaaact attctgttta
     1021 taaaaataaa gaaaggagta accaatggat atgaaatata aagccttgat tattgccctt
     1081 atatgtttat gggcactata taacgccgta aacagtcgca gaaaaatttc agggcttgaa
     1141 ctaatttcag ttagctatat agcatactgg ggatttcagt attttctaac tcactaacaa
     1201 aactaaacga gtgcggagaa agctggtaat caagactcta aaagcctttt ttaataaaaa
     1261 accgcaggtt aaataataaa gatacctttt tttcaaaagt cggcaatttg agattgactc
     1321 tcatagccta aaggtcgcac gcttaagtct tgattctcca tcaaacggaa aatgcattat
     1381 gataataagt cttccgtttg atggaaagaa tttaagtaaa aatacctgta cctactttat
     1441 ttgcaagtct tttatatagt ttagcaagtt accagcctgt ctatttttct ggccagccga
     1501 tctgcccctg cccttcacgc accgcccaat agatgatccc gccccaattc caggaacgct
     1561 ggtattgagg attgctgatg ctgccggcat cgtctatgcg tcgcaaggtc aggttggtat
     1621 cccagtaggc atcgggcgtg ccatcattgt tgatgtctgc cattctcccg gcaggcaggt
     1681 ttgccacagc ggaaggcggg atttccccgc cgctggaacg gtttctccat tcataaccag
     1741 tgatgccacg atgtccggtt ccgccgccgc agttgtaatt acctctgccg ttcggaccgg
     1801 gaagacagcc ttcgatggct tcatattcct gataacgtaa ttcccaaa
//
LOCUS       DAOQJE010000787         1843 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_53980_length_1843_cov_4.755034, whole genome shotgun sequence.
ACCESSION   DAOQJE010000787 DAOQJE010000000
VERSION     DAOQJE010000787.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1843)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1843)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1843
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_53980_length_1843_cov_4.755034"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..333
                     /locus_tag="PLT08_16525"
     CDS             <1..333
                     /locus_tag="PLT08_16525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE06134.1"
                     /translation="LAFVPDILLIPLILSMVISIFQNLVLTASGFDGLGVYLSEHLRP
                     LWKKHGILQPRGILVYDICLPNVLKGNLINNRGIDLFNLSGPYSLIPLMIAECSFLFI
                     FLKLRFQK"
     gene            642..896
                     /locus_tag="PLT08_16530"
     CDS             642..896
                     /locus_tag="PLT08_16530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE06135.1"
                     /translation="MQARTCSKCGSHNVYKNTSKNWHQDGVVLQMIAVDRFTYRFETD
                     AYLCLDCRYLEILVSETNTMYGDQKTLTEVVQASSNWAKA"
     gene            1011..1241
                     /locus_tag="PLT08_16535"
     CDS             1011..1241
                     /locus_tag="PLT08_16535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE06136.1"
                     /translation="MNKSLFEEKWKLLRGEVNTRWSLMVEYDLLKVDKAEVKFDKFVT
                     MLQVKYGYTRQKAKEEIVKLWSDYESNHRSKN"
     gene            1251..1397
                     /locus_tag="PLT08_16540"
     CDS             1251..1397
                     /locus_tag="PLT08_16540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE06137.1"
                     /translation="MNSTPEKTQVKHKRPSKGVRKHNRKVKQEARRVSVPGAEVKKSK
                     RSAS"
     gene            1526..1786
                     /locus_tag="PLT08_16545"
     CDS             1526..1786
                     /locus_tag="PLT08_16545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE06138.1"
                     /translation="MRNGVCTACGSHNVYKSANNSWYQSGVKVAVLGDKVNEVFMTEA
                     YVCLDCRHLELVVEENAAAIFGKGKAFKDAVPASTNWKKVSG"
BASE COUNT          568 a          402 c          410 g          463 t
ORIGIN      
        1 ttggcatttg tgccggacat actccttatc cctttgatcc taagcatggt aatctccatt
       61 tttcaaaacc ttgtcttaac cgcaagcgga tttgatgggc tgggcgttta tctcagtgaa
      121 catctgcgac ctctttggaa gaagcatggt attttgcaac ctagagggat ccttgtgtat
      181 gacatctgcc tgccgaatgt acttaaaggg aatttgatta acaacagagg catcgatctt
      241 ttcaacctat cgggacctta tagcttaatc ccattaatga ttgcagagtg tagctttcta
      301 tttatctttc taaaattaag gtttcaaaaa taagaaccaa atgcaaacta gtgaatataa
      361 tgggtacgaa gaaggtgcag cggcgatgta tttcagcccc aacacctctt tggcaaatat
      421 catcactccc tgtgccgatt caaggaatga aaaacggtct cgggatgtta tatccgcgag
      481 accgtttttt tcttgtctat ctttattccc agaaaagggt acaatgaaat caggcaacta
      541 gacggtaacg ggggatgcaa tcacatcaac ataccttcca gaaaacctta ctcgccctat
      601 taagcgagac ggatacactc aaatctgaga aggagatttc catgcaagct cgaacatgct
      661 caaaatgtgg ttcccacaat gtctataaaa atacaagtaa aaactggcat caggatgggg
      721 ttgtcctgca aatgatcgcc gtagatcgtt tcacctatcg ctttgaaaca gatgcttatc
      781 tctgcctcga ttgcaggtat ttggaaattc tggtctctga aacaaacacc atgtacggag
      841 atcaaaaaac acttacagaa gtcgttcaag ccagtagcaa ttgggcaaaa gcctgacata
      901 agagtttgaa tctttttcat tcctattcat gggggagatg gatgggcacg aaatcacaaa
      961 actcccaacc cgtattctct aaaacgcgct ttcttgagga ggagcgcctt atgaacaaat
     1021 ccctgtttga agaaaaatgg aagctgctac gcggggaggt caacacccgt tggagtttga
     1081 tggtcgaata cgacctgctc aaagtggata aagctgaggt gaagttcgat aagttcgtca
     1141 ccatgctgca agtcaaatac gggtacaccc gccaaaaagc caaagaagag atcgtcaaac
     1201 tttggtcaga ttatgaatcg aatcatcgaa gtaaaaacta aaggagccac atgaattcaa
     1261 ccccagaaaa aacacaggtc aagcacaaac gtccctccaa aggggtacgc aaacacaacc
     1321 gcaaggtcaa gcaagaggcg cgacgcgtca gtgtgccagg ggcagaggtc aaaaagagta
     1381 agcgttcagc ttcgtaaaac aggacggtct caggtttatc cgatgagacc gtttttattt
     1441 tccacatgac caaagaggga atacccactc ccctgcttct ctatataatc agcccattat
     1501 caatatcaaa ggagaaactt tagtaatgag aaatggagtt tgtacggcat gtgggtctca
     1561 caatgtctat aaaagtgcga acaattcctg gtatcaatct ggggtcaaag ttgcagtgct
     1621 cggagataag gtcaacgaag tgtttatgac ggaagcgtac gtctgcctcg attgtcgtca
     1681 tcttgaattg gtggtggaag aaaacgccgc cgccatcttc ggcaaaggca aagccttcaa
     1741 ggatgccgtc cctgccagca cgaactggaa aaaagtatcc ggttaatgtt cgttttcagg
     1801 cttaaacaaa aaacggtctt gggtgaaata cccaagaccg ttt
//
LOCUS       DAOQJE010000788         1842 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_54019_length_1842_cov_5.517627, whole genome shotgun sequence.
ACCESSION   DAOQJE010000788 DAOQJE010000000
VERSION     DAOQJE010000788.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1842)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1842)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1842
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_54019_length_1842_cov_5.517627"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1337
                     /locus_tag="PLT08_16550"
     CDS             <1..1337
                     /locus_tag="PLT08_16550"
                     /inference="COORDINATES: protein motif:HMM:NF024581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HNE06139.1"
                     /translation="YGETYQAQAWKPVLTFEHERGLEGAVESCNGQGVCRKTNGVMCP
                     SFQATRDEAFSTRGRANLLRALMRSDVDFSVPRNSELELATYQSLDLCLACKGCTSEC
                     PSGVDMPKLKYEFMNQYYKSHPRHLRDYLFGYFHVATKLLMPFAPMANWFMQMGWSKK
                     IVARVLGISEHRPFPKFVRGTRQAHEMQFDKQVIFVSDVFSHYIEPEVEEAVVEILNS
                     LGYEVKILPTIGAGASLLSKGFLNAAQRHAEKVLAEIKRLDSGAGLSVVGCEPPELYC
                     LKHEYESLLPNRKEEIESIKKRTWLVDEFILRVVNEAKMDISHVASSEQNNRLTLHPH
                     CHHRAEGLAADGLPSGVSATVDMLKQFGYEVDVIDAGCCGMAGTFGYDAEHYELSMKV
                     GELGVLPKVRDKKLENRELVASGAACRMQIKQGADVDARHPLLLVKERLISK"
     gene            1344..>1842
                     /locus_tag="PLT08_16555"
     CDS             1344..>1842
                     /locus_tag="PLT08_16555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE06140.1"
                     /translation="MLNRISNFFYAVSTGWLTLMGLVVFILFMIFVLPQQAQKAEAYS
                     GGTSPDTSYIYSASDLYQMAESYGAEGRAAYIYARFTFDLVFPLAYLFFLTTSISWLL
                     ARGLSENSRWRMLNLFPLAGAIFDYLENISTSLVLARYPSQTPVVDVLAPVFTAIKWV
                     FVNGSF"
BASE COUNT          465 a          350 c          478 g          549 t
ORIGIN      
        1 ggtatggcga aacgtatcag gcgcaggctt ggaagcctgt attgaccttt gaacacgagc
       61 gaggtttgga aggtgcagtt gagtcatgca atgggcaagg cgtatgtcgt aaaacgaacg
      121 gtgtgatgtg cccatcgttt caggcaacgc gcgatgaagc ctttagtacg agagggcgag
      181 ctaatttact gcgagcattg atgcgctcgg acgttgattt ttcagtgcct cgaaattctg
      241 aacttgaatt agcaacctat cagtcactcg atctttgcct tgcttgtaaa ggctgcacat
      301 ccgaatgccc aagtggtgtg gatatgccga aactcaaata tgagtttatg aatcaatatt
      361 acaaatcgca tccgcgccac ttgcgagatt atctgttcgg ctattttcat gtcgctacaa
      421 aattgttgat gccatttgcc cctatggcaa attggtttat gcaaatgggc tggtcaaaga
      481 agatcgtggc acgtgtgttg ggtatttcag aacacagacc ttttccaaaa tttgttcggg
      541 gtacacgcca agcgcatgaa atgcaatttg ataaacaagt aatttttgtt tcggatgttt
      601 tctcacatta tattgaacct gaggttgaag aggctgttgt tgagattttg aactcgttgg
      661 ggtatgaagt gaaaattttg ccaactattg gagcaggcgc ttctttgctt tcgaagggat
      721 ttctcaatgc tgcacagcgt catgcagaaa aagtgttggc agaaattaaa cgcttggata
      781 gtggagcagg tttgagcgta gttggatgcg agccgcccga gttgtattgt ctcaaacatg
      841 aatatgaatc gttattgcca aatcgcaaag aggaaattga atccataaag aagagaacgt
      901 ggttagtgga tgaatttatt ttgcgtgtag tcaatgaggc taaaatggat atttcacatg
      961 tggcttcttc tgaacaaaat aaccgcctca ctctccaccc gcactgtcac caccgcgcag
     1021 aaggtctcgc cgctgacggt ttaccttcgg gcgtttcagc tacagtagat atgctcaagc
     1081 aatttggcta tgaagtggac gtaattgatg caggttgttg tggcatggca ggcacctttg
     1141 gctatgatgc tgaacattac gagctatcaa tgaaagtagg ggagttgggt gtgttgccga
     1201 aagtgagaga caagaaatta gaaaacagag aattggtggc aagcggtgcg gcttgtcgta
     1261 tgcagattaa gcaaggtgca gatgtggatg cacgtcatcc attgttattg gtaaaagaac
     1321 gacttatatc caagtgaggt gctatgttga accgaatttc aaatttcttt tatgctgttt
     1381 ctacaggctg gctgaccctg atggggttgg ttgtctttat cctttttatg atcttcgtgt
     1441 tgcctcaaca ggctcaaaaa gcggaggcgt atagcggcgg aacttcacca gacacttcat
     1501 atatttactc tgcaagcgac ctgtatcaga tggcagaaag ttatggcgca gaagggcggg
     1561 ctgcttatat ttatgcgcgt ttcacattcg atttggtgtt tccgctggct tacttatttt
     1621 ttctgacaac atctataagc tggttgttgg cgcgtggact ttctgaaaac tcccgctggc
     1681 ggatgttaaa tctctttccg cttgcgggag ctatttttga ttacttggaa aatatttcca
     1741 cttcactagt gttagcacgt tacccgtctc agacgcctgt ggttgatgtt cttgcaccgg
     1801 tgtttacggc gattaagtgg gtctttgtga atggaagttt tg
//
LOCUS       DAOQJE010000789         1839 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_54230_length_1839_cov_4.792601, whole genome shotgun sequence.
ACCESSION   DAOQJE010000789 DAOQJE010000000
VERSION     DAOQJE010000789.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1839)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1839)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1839
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_54230_length_1839_cov_4.792601"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(120..851)
                     /locus_tag="PLT08_16560"
     CDS             complement(120..851)
                     /locus_tag="PLT08_16560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE06141.1"
                     /translation="MNKKNMLVSAFAATFVIVIAGIVAMVVLYFQPKTVIAKSVEPVS
                     NYAESSTVVNVPSLENSNAAEVKYPLAQEINGIKMEVTGTAMEGEYFTTDVCYDLPAP
                     KTGYEFTLGGQTQKSIALTNAKNETIMIYSWKIIGGFNQDANGNFQGNCARLYFPVLS
                     DTNLDGLTLTISRMSTPVADFPDCEKAQQKLDEAKQKIKVECYENGGFGVSEKPDDMS
                     DQEAREIALEAFIEIVEGPWVFKLK"
     gene            complement(940..1557)
                     /locus_tag="PLT08_16565"
     CDS             complement(940..1557)
                     /locus_tag="PLT08_16565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE06142.1"
                     /translation="MKPLFKITSGFSLDLVMILIVAGILVSCSQKSVVVGSTRNQQLP
                     IVVRSTEVTSIEQHKGFETTYGIIVAVKALCKDDVNTILYLKTNFDASLWHLNEKEFY
                     PFGGAYFETSILFLENGEMFSMYASGKRDEPIFDLQNNMVSTMQTFVFPKIPSEESTF
                     TIKAKVSLHDLPVTYIPPIKVDFLEPGIIEIPMEYIVSAEIGECP"
BASE COUNT          541 a          401 c          340 g          557 t
ORIGIN      
        1 agcaaacggt ggtaaccttc cccaatgggg aaagcgtgat cgtaaaagaa gaaacagcgt
       61 gtatagtgga gatgtaaaaa gagcggactg ctaaaaacag tccgctcttt ttgttcaaat
      121 tacttcagct taaataccca gggaccttcc acgatttcaa taaaagcctc taatgcaatt
      181 tctctcgctt cttgatcgct catatcatcg ggcttttcag atacgccaaa cccgccattc
      241 tcataacatt ccacctttat tttttgcttt gcctcatcca acttttgctg ggctttctca
      301 caatcgggaa aatctgccac aggagtggac atgcgcgaaa tggtgagtgt cagaccatcc
      361 agattggtat cagacaaaac agggaaatat aaacgggcgc aattcccctg gaagtttcca
      421 tttgcatctt gattgaaacc tccaatgatc ttccaactat atatcatgat ggtttcattt
      481 ttagcattgg tcaatgcaat ggatttttgg gtctggcctc ccagtgtaaa ttcataccct
      541 gtttttgggg ctggaagatc gtagcataca tctgtggtga aatactcccc ttccatagcc
      601 gtgccggtca cttccatttt gataccgttt atttcctggg caagcggata tttcacttcg
      661 gcggcatttg aattttctag cgatggaaca ttgacaaccg ttgaactttc agcgtagttt
      721 gaaaccggct ctacagattt agcgatgact gtcttgggct ggaaatataa aaccaccatc
      781 gcaactattc ctgcaataac aattacaaag gttgctgcaa acgcagaaac gagcatgttt
      841 tttttattca cgagacattc cttattattc tccttaatca gtgaacctgt cacccactcc
      901 gtaccaggct tgcctaaaaa caatcgtgtc actgtaactt tatggacact ctccaatttc
      961 tgcagacaca atatactcca ttggaatttc gataattcca ggctccaaaa agtcaacttt
     1021 tattggggga atataagtaa caggcaaatc atgtaaagat acttttgcct tgattgtaaa
     1081 tgtggactct tcagaaggta ttttcggaaa aacgaaggtt tgcattgtac tgaccatgtt
     1141 attttgcaaa tcaaaaattg gctcatcacg ttttccactt gcatacattg aaaacatttc
     1201 gccattttct agaaacaaaa tacttgtctc aaaataagct cctccaaatg ggtaaaattc
     1261 cttttcattt aaatgccaga ggctcgcatc aaaattagtt ttcaaataga gtatagtgtt
     1321 cacatcatct ttgcacagtg cttttactgc aactatgatg ccatatgtag tttcaaatcc
     1381 tttatgttgc tcaattgatg tgacctcagt tgatcgaaca acaatgggca attgttgatt
     1441 ccgggttgag cccacaacta ctgatttttg agaacaggag acaagtatgc ctgcaacaat
     1501 caatatcatt accaaatcaa gagaaaaacc agatgttatt ttgaataatg gtttcatttt
     1561 cctccagaac tatttctcga tttatctaat tcgcgcctgt acaaaatgtg ttggatacac
     1621 caatcgggac agcaataggc ccagaaacca aggcactccc acaggaacgt gtcggtgttg
     1681 ctgcgtatgc gtggagtgta cacaatgttg tcattgaata ttattcttgg caagcgaata
     1741 ttccgaatat aacaatagct tgacaatcat tcaacattag caaaggaggt tatagcattc
     1801 ctgaatgtcc tcatcaatta cggaaatccc cactctttt
//
LOCUS       DAOQJE010000790         1837 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_54360_length_1837_cov_3.604938, whole genome shotgun sequence.
ACCESSION   DAOQJE010000790 DAOQJE010000000
VERSION     DAOQJE010000790.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1837)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1837)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1837
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_54360_length_1837_cov_3.604938"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            49..1572
                     /locus_tag="PLT08_16570"
     CDS             49..1572
                     /locus_tag="PLT08_16570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE06143.1"
                     /translation="MMLRNEISPTIRIKLVYASAALACASALVVQIWFATAGTWFTLP
                     TFPYPYDHYYNQLADAFLQGHTYLETPVNPLLLELPDPYNPGARGNLEFLWDTSLYEG
                     KYYLYWGPMPSVIIMVIKLIYPGFIGDNLVVFAAVAIITIFQTLLLTWLWTQYFQRLP
                     IWTLFIGVLMAGLIIPLNIMNNRPEIYEGAIISGQAFLMGGIYFAVLAVRPTEIFPRH
                     LVAASMLWGCAVASRTIVVFEVIFASMLIGLLILNRPVGWAWRLKRLIAFGLPLALFA
                     FGLGAYNFVRFGSAFEFGYKYQLTMFDFSKIGDELFSTRYMFPSMENYVLTAPHRIKT
                     FPYFRPKAGDEIEFSVKSPDLYYIETITGVIYTFPFVIFALVPILRTLTKGVKLGKNT
                     DTSEYILQWVVVLLSGMGVIAVTQLLSFFYTTMRYLADVTPILSVLALMGFWFGYQTV
                     SSNKALRFAYAGIGVFLAGISIIVPNMLALVISERISTYSPNVLPALDALFKSVFLK"
BASE COUNT          460 a          364 c          410 g          603 t
ORIGIN      
        1 cttagcaatg tataaatcta ctatggaata aataaaccta taaactatat gatgttaaga
       61 aatgaaattt cgccaaccat acgaataaag cttgtgtatg catctgcagc tcttgcatgt
      121 gcgtctgctc ttgttgtaca aatctggttt gccacagcag gtacatggtt tacgttgcca
      181 acgttcccat acccttacga tcattactac aatcaattgg cagatgcatt tcttcaaggg
      241 cacacctatc ttgagactcc tgtcaatcct ttacttcttg aactcccaga tccttataac
      301 ccgggagcac ggggcaacct tgaattttta tgggacacga gtttgtatga agggaaatat
      361 tatttgtatt ggggaccaat gccctcggtc attattatgg tgatcaagtt aatatatccg
      421 ggttttattg gcgataattt ggtcgtgttt gctgcggtag ctattattac gatatttcaa
      481 actttattgc ttacgtggct ttggacccaa tatttccaac gtttgcctat ttggacactt
      541 ttcataggcg ttttgatggc tgggttgata attcccttga atattatgaa taaccgtccg
      601 gaaatttatg agggtgctat catcagcgga caggcttttt tgatgggagg gatctatttt
      661 gcagtgcttg ccgttcgccc tactgaaatt tttccacgcc atctggttgc agcatctatg
      721 ctttggggat gtgcggtcgc ctcacgtacc atcgtcgtct ttgaagtaat ctttgcatca
      781 atgttgatcg gacttctcat actgaaccgc cctgttgggt gggcttggag acttaagaga
      841 ctcattgcct tcggtctgcc actggcattg tttgcgtttg gtttgggagc ctataacttt
      901 gtacgctttg ggtctgcctt tgagtttggg tataaatacc aactaaccat gttcgatttt
      961 tcaaaaattg gagacgaatt attctccacc cgatatatgt tcccaagtat ggaaaattat
     1021 gtcctaactg caccgcaccg cattaaaacg tttccctatt ttagacccaa agcgggagat
     1081 gaaattgaat tttctgttaa gtcgccagat ttgtattata tagaaacgat cactggtgtg
     1141 atttacacct tcccttttgt aatttttgct cttgtgccga tcttaagaac gcttacgaaa
     1201 ggagtcaagc tgggtaaaaa tacggataca tcagaatata ttctgcaatg ggttgttgtt
     1261 ttattaagtg gtatgggggt gattgccgtc acccagcttc tgtctttctt ttatacaacg
     1321 atgcggtatc ttgcagatgt gacaccgatc ttatctgtac tggcattgat gggtttttgg
     1381 tttggatatc aaacagtttc ttcaaataag gcactaaggt ttgcctacgc tggcataggt
     1441 gtttttttgg caggaattag cataatcgtc cctaatatgc ttgctttggt tatttcggaa
     1501 agaatcagta cgtatagtcc aaatgtgcta cctgccttgg atgccttatt taagtctgtt
     1561 tttctaaaat gaaataattc aaatgaagca atctcatgac tagcgtagga gattgcttcg
     1621 tcgcgaagag caagagcgct cctcgcaatg acgaggaatg aagaggtttt tatgaacata
     1681 caacgaactc aaattggcta cctcgtcggt ggcggactta aagaaaattt ccgcgtgcgt
     1741 cttaccgttt ctccgcagga gattcaggag ggggcgtttg tcgtcatcca gagcggatgg
     1801 tggaattatt atgggatcgt gaccgacatc caactgg
//
LOCUS       DAOQJE010000791         1831 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_54688_length_1831_cov_4.958896, whole genome shotgun sequence.
ACCESSION   DAOQJE010000791 DAOQJE010000000
VERSION     DAOQJE010000791.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1831)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1831)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1831
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_54688_length_1831_cov_4.958896"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            246..1271
                     /locus_tag="PLT08_16575"
     CDS             246..1271
                     /locus_tag="PLT08_16575"
                     /inference="COORDINATES: protein motif:HMM:NF013357.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003796 - lysozyme activity [Evidence
                     IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA];
                     GO_process: GO:0016998 - cell wall macromolecule catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GH25 family lysozyme"
                     /protein_id="HNE06144.1"
                     /translation="MNLVHGVDVSYWEPKIDWQALRSQGFKFALIRATSGVGYVDPKF
                     KSHWEGARKEGLLRGAYHYLIANQDPKQQADLFISNISADRGELPPIIDLEDKYNESA
                     KNTQIIDTCKAILDRVEKAFGVKPMIYSRTQYLNLHVSRNGAAPSWAKDYPLWLAQYP
                     YVFDANTMPNKNMPTQATGWQDWLFWQYSETTLVDGVTSDNGQPTECDLNWFRGTEEE
                     LYAFANVKPKEPVSYTVKSGDTFKAIAEKYKITVDELLAANPQLLADGTKLSIPVPTI
                     SKPIVSDNSTTNKATSSTRTHTVKQGDTIGTIAQKYGVTVDAIKALNPQLTNPNLIYV
                     GQVIVIP"
     gene            complement(1344..>1831)
                     /locus_tag="PLT08_16580"
     CDS             complement(1344..>1831)
                     /locus_tag="PLT08_16580"
                     /inference="COORDINATES: protein motif:HMM:NF012379.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HNE06145.1"
                     /translation="DDAAKYLIDLYEKETDPEKKELLKFEWGMDFGAPHETELTKLHD
                     KPVFVYGYPSAVKAFYMEPWENRPEVCKSVDLLAPEGYGEICGGSERMSDPDKLLARI
                     QKEGLPEEAFKWYLELRKYGSVPHAGFGMGTERVTAWMCGIEHIREAIPFPRTIKRIY
                     P"
BASE COUNT          456 a          435 c          478 g          462 t
ORIGIN      
        1 taccaagcaa cgccgccttg gctcaggcct atcgcgttgt tactgtaaag ttaaggaccc
       61 ttttagtgag tattggctca aaacggaagg gcgatatcac gagcaccgca gggtgcgatt
      121 cttataccat actctgcgag acaaggaaag atactctcta agtatgctct taacgaagag
      181 attgattttc tcaaaaaatt gtgctaggct aatttgagca gtgtttttat tcaaaggaga
      241 gaacaatgaa tttagtccat ggcgttgacg taagttattg ggagcctaaa atcgattggc
      301 aggctctgcg ttctcagggg tttaagtttg ctttgattcg tgccacttct ggggtggggt
      361 atgtggaccc caagtttaag tctcattggg agggagcacg caaagaaggt ctcttgcgtg
      421 gggcgtatca ttacctgatc gccaaccaag acccgaaaca acaagcagac cttttcattt
      481 ccaacatcag tgcagaccgc ggggaactgc cgcctattat tgaccttgaa gataaataca
      541 atgagagtgc taaaaacacg cagatcattg atacatgcaa agcgattcta gaccgggtgg
      601 agaaggcgtt cggtgtaaaa ccgatgatct attcacgcac ccagtatttg aatttgcatg
      661 tctcacggaa tggagcggcg ccttcgtggg cgaaggacta tccgctttgg ttggcgcaat
      721 atccatatgt gttcgatgcc aacaccatgc caaataaaaa catgcctact caggcgacag
      781 gttggcaaga ttggctcttc tggcaatatt ctgaaactac gctagtagat ggtgtgacga
      841 gtgataatgg tcagcccacc gaatgtgacc tgaactggtt ccgcggaaca gaagaggaac
      901 tctacgcatt tgcgaatgtc aagccgaaag aaccggtcag ctacacagtg aaaagtggcg
      961 acaccttcaa agcgatcgcc gagaaatata agatcacggt ggatgaattg ctggcggcaa
     1021 atccgcagtt actggcagat ggcacgaaac tttccatccc tgtgccgaca atttcgaagc
     1081 caatcgtcag cgataattcc acgacgaata aggcaacttc ctccacgcgg acacataccg
     1141 taaagcaggg cgatacgatt ggtaccatcg ctcaaaaata cggcgtgacc gtggatgcca
     1201 tcaaagctct caacccgcaa ttgaccaatc ccaatttgat ctatgtgggt caggtgattg
     1261 ttattccgta attggattaa atataaacat ccccgtcgct tcgttcgacg gggatgtttg
     1321 attctgtttc ctgaaagttt ttcttacggg taaattctct tgatcgtgcg tgggaagggg
     1381 atcgcctcgc ggatgtgttc gatgccgcac atccaggcgg tgacgcgttc tgtgcccatg
     1441 ccgaagcccg cgtgggggac ggagccgtac ttgcgcagct ccagatacca cttgaaggct
     1501 tcttcaggca gaccttcctt ttgaatgcgt gccagtagtt tatccgggtc gctcatgcgt
     1561 tcggagccgc cgcagatttc gccgtagcct tcgggggcga gcaaatccac gctcttgcag
     1621 acttcggggc ggttctccca cggttccatg taaaaggctt tgaccgcgct cgggtagccg
     1681 tagacgaaga ccggtttatc gtgcagtttg gtgagttcag tctcgtgcgg agcgccaaag
     1741 tccatgcccc attcaaattt gagcagttct ttcttttcgg ggtcggtctc tttttcgtag
     1801 aggtcgatca gatatttcgc cgcgtcgtca t
//
LOCUS       DAOQJE010000792         1830 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_54741_length_1830_cov_4.579718, whole genome shotgun sequence.
ACCESSION   DAOQJE010000792 DAOQJE010000000
VERSION     DAOQJE010000792.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1830)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1830)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1830
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_54741_length_1830_cov_4.579718"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            217..723
                     /locus_tag="PLT08_16585"
     CDS             217..723
                     /locus_tag="PLT08_16585"
                     /inference="COORDINATES: protein motif:HMM:NF024698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNE06146.1"
                     /translation="MTDRSEYLLKLSEVPNVNTSPHESTFLIRTTQVKDASLLAELMI
                     DAYRGTIDYDGETFDDALSEVNAFFAGERGGQPWLNISFLAFSDSQLVGACLIGEWKE
                     RQSPIIAYLMTSANWKNRGLGRQLLNQGLKEIGDKGFTEVRAVITNGNTPSESLFLKL
                     GFQKVQAL"
     gene            1040..1651
                     /locus_tag="PLT08_16590"
     CDS             1040..1651
                     /locus_tag="PLT08_16590"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF012521.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HNE06147.1"
                     /translation="MKDEVYVTFLRHGRSRADDEEVHEGRYDSPLTDVGRSQAQSRAQ
                     EFLYRNFHFDKIVSSTLQRAHETAIIIGQLLNISVETDSDWMEMDNGPLAGLPRDVAE
                     EQYPRPVFRNPYEPFCGTGESDWEIYCRGARAVEKIIRLGTGNYLVVAHGGILNSAMR
                     TIVGAQPLVNQHGIAFGFGDTGYARLVYRPSEHLWCLLEFNVG"
BASE COUNT          475 a          421 c          455 g          479 t
ORIGIN      
        1 tactacctga cgctggggac tgcccgaagg gcgtgcgcca atctcaagca gtttcctacg
       61 ccttagcctt tttccctgga ctgtcgtcca ggacaggctg gctggacggc ttcgccgtcc
      121 ccaccccagc gcaggtaccc acagggcaag ccgcaaaccg ttcggcggct tcgtaaatta
      181 gaatacttgt caaaaccaaa ttaaggaaga gattttatga ctgatcgcag tgaatattta
      241 ctaaagttat ccgaagttcc aaatgtaaac acatctccgc atgaatctac atttctgatt
      301 aggacaacac aagtaaagga tgcctcttta ttagcagaat taatgattga tgcttatcgc
      361 ggaacaattg attatgatgg ggagaccttt gatgatgcgc tatcagaagt caatgctttt
      421 ttcgcagggg aacgcggtgg tcagccttgg ttgaatatat cttttttggc attttctgat
      481 tctcaactgg ttggcgcctg cctaattgga gaatggaaag aaagacaatc gccaatcata
      541 gcgtatttga tgacaagtgc taattggaaa aatcgtgggc ttgggcgtca attgctaaat
      601 caaggattga aagaaatagg tgataaaggt tttacagaag taagagcagt aattactaat
      661 ggaaatacgc cttctgaaag tctattctta aaacttgggt ttcaaaaagt gcaagccttg
      721 taaataagca acgctgccga acaaagcgtc ccgatagtgc tgggatgata tgcacctgac
      781 gctggggatt gtcaagcgcc aatgcgcttg actgggcgat gctcgctgga ggctcgctcg
      841 cccgatctgc ggcaatctca agcagtttcc tacgccttgt catttttccc tggactgtcg
      901 tccacctgtg cctgcggcaa ggtgcaggca ggacaggctg gttggacggc ttggcccggt
      961 ccgcgagaca gggcaggtac ccacagggca agccgcaaac cgttagccca cttctttgaa
     1021 aatgaagtca ggattgaaaa tgaaagatga agtctatgta accttcttac gacatgggcg
     1081 ttctcgtgct gatgatgaag aagtccacga aggcagatac gattctccgc ttaccgatgt
     1141 agggcgctct caggctcaat cccgcgcaca agaatttctc tatcgcaatt ttcactttga
     1201 taaaattgtg tcaagtacac tccaacgcgc tcacgaaaca gcaataatca ttggtcaatt
     1261 gctaaatatc tctgttgaaa cagattcaga ttggatggaa atggataacg gacctcttgc
     1321 aggcttgcct cgtgatgttg cggaagagca ataccccaga ccagtttttc gtaatcccta
     1381 cgaacctttt tgcggcacag gtgagagtga ttgggaaata tattgtcggg gagcaagagc
     1441 agtagaaaaa attattcgac tcggcactgg aaattatttg gttgttgctc acggcggtat
     1501 tctaaactct gctatgagaa cgattgttgg cgcacaacct cttgttaatc aacacggcat
     1561 agcatttgga tttggcgaca ctggatatgc tcggttagtc tatcgtcctt ctgaacatct
     1621 ttggtgtctt cttgagttca acgtgggcta acacagcgtc ccgatagtgc tgggatgata
     1681 tgcacctgac ctcttctgtc ctttggacat cttcccctaa aggggaagac tcagcctttg
     1741 cctttctctt gctggacaga tccgcccggt ccgctagaca gggcaggtac ccgcaggtca
     1801 agccgcatgc cgttaggcgg cagatatgtc
//
LOCUS       DAOQJE010000793         1827 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_54928_length_1827_cov_4.845937, whole genome shotgun sequence.
ACCESSION   DAOQJE010000793 DAOQJE010000000
VERSION     DAOQJE010000793.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1827)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1827)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1827
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_54928_length_1827_cov_4.845937"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1537)
                     /locus_tag="PLT08_16595"
     CDS             complement(<1..1537)
                     /locus_tag="PLT08_16595"
                     /inference="COORDINATES: protein motif:HMM:TIGR03663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03663 family protein"
                     /protein_id="HNE06148.1"
                     /translation="MDSKRNWLDRPIHPTLPAITNEIILFVVIILLAIVTRFYNLGAR
                     VMSHDESLHTYFSWLLYQGRGYEHTPMMHGPLQFHLIALSYFLFGASDYTARIPAVLF
                     NLMTIWMVWYWRRYLGRTGALIAGFLLVISPFMLYYGRYVREDSYPIFSGILMLYAVL
                     RHLESGGKKYLYILSTALLIHYVAKETSFIYTAQLLIYLAIYFIVQVSRTPWKGATSD
                     YRTFIIALCVTILLVGATAGYVLYSRTPETTNAAQTAVPANPDAAAQTSPESALSPLT
                     IILGIAAVLAFVVTAYFLIRGYTWERLRSNRAFEMLMITGTLVLPQLSPLLINLTKVT
                     IPTTAPEITALAGDWRAILTIGACLLVTFLLAIAIGMIWNPKTWWKATLIFWVPYVIL
                     YTAIFTNPNGFFTGTIGSLGYWIVQHDVQRGSQPWYYYLLIQVPVYEFLPALGLILAL
                     ILGLRRKVSLPAAYEEDMIDGTFDGNWEEGKQPAPTQEQQEAYTREMNFVNMFSLLVW
                     WSFI"
     gene            1754..1825
                     /locus_tag="PLT08_16600"
     tRNA            1754..1825
                     /locus_tag="PLT08_16600"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1786..1788,aa:Met,seq:cat)
BASE COUNT          486 a          363 c          534 g          444 t
ORIGIN      
        1 tgatgaaact ccaccatact aaaaggctga acatattcac gaaattcatt tcgcgggtgt
       61 aagcttcttg ctgttcttgg gtaggagcag gttgtttgcc ttcttcccaa tttccgtcga
      121 atgtgccgtc gatcatatct tcttcatatg cggcaggaag agatacttta cggcgaagcc
      181 ccaagatcaa ggcgaggatc aagcctagtg ccggtaggaa ttcgtacacc ggaacttgaa
      241 tgagaaggta gtaataccaa ggttgactgc cgcgctgcac atcgtgttga acaatccaat
      301 accctaacga gccgatggtg cccgtgaaga aaccattggg gttggtaaag atcgcggtgt
      361 ataagattac gtaaggtacc caaaagatca gggttgcttt ccaccatgtt ttggggttcc
      421 agatcattcc aatggcaatt gcgagtagga aggttacaag tagacaggcg ccaattgtga
      481 gaatagcacg ccaatctccg gctagagctg tgatttcagg tgcggtggtt gggatggtaa
      541 ctttggtgag gtttataagc agtggggaaa gttgcggaag aacaagggtg cctgtgatca
      601 tcagcatttc aaaggcacgg ttcgagcgga gccgttccca tgtgtagccg cggatcaaga
      661 agtaagcggt gacaacgaat gcgagcacag cagctatccc gaggataatg gtcaatggag
      721 agagcgcaga ctcgggcgag gtttgagccg cggcatctgg gttggcgggg acggctgttt
      781 gcgccgcgtt ggttgtttct ggcgtgcgac tgtaaagcac atatccggca gtggcaccga
      841 ctaggagaat agtgacacag agtgcaatga taaatgtgcg gtaatcactg gtggcgcctt
      901 tccaaggggt gcgtgagacc tgaacaatga agtaaattgc cagatagatc agcaattgtg
      961 ctgtatagat gaaggaggtc tcttttgcga cataatgaat gagcaatgcc gttgagagga
     1021 tgtatagata ttttttgccg ccgctttcaa gatgcctgag aacggcgtat aacatcaaga
     1081 tgccggaaaa gatcgggtag ctatcttcgc ggacataacg cccgtagtaa agcatgaaag
     1141 gcgagatgac taaaaggaag cctgcaatga gagcgccggt gcgacctaaa taccgacgcc
     1201 agtaccagac catccaaatg gtcatgagat tgaatagaac agcagggatg cgggctgtgt
     1261 agtcgctggc accgaagagg aaataggaaa gcgcgatcag gtgaaactgc agcggtccat
     1321 gcatcatggg ggtgtgttca tagccgcgcc cttgatacag aagccaggag aaataggtgt
     1381 gtaagctttc gtcgtggctc atgacacgtg cgccaaggtt gtaaaagcgc gtgacgatgg
     1441 caagtaagat gatcacaaca aataggatga tctcatttgt aatcgcaggg agtgtgggat
     1501 gaatgggacg gtcgagccag tttcgtttcg aatccattga tggcaccttg agtagagtga
     1561 aatattttcg ataaggctta aataatagtc ttttaagttt aatatgacgt gagaaggtat
     1621 tataacagga ggggttgaaa atcttctttg ccttggtttt gggcttaacc tgcaaggttc
     1681 atggaaatag gttttgtttc ttgcttgact tttctatggt tacttggtaa tatcggcggg
     1741 ctctactaac cggtgcgggg tggagcagtg gcagctcgtc gggctcataa cccgaaggtc
     1801 acaggttcaa gtcctgtccc cgctact
//
LOCUS       DAOQJE010000794         1823 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_55171_length_1823_cov_3.586538, whole genome shotgun sequence.
ACCESSION   DAOQJE010000794 DAOQJE010000000
VERSION     DAOQJE010000794.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1823)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1823)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1823
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_55171_length_1823_cov_3.586538"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..381)
                     /locus_tag="PLT08_16605"
     CDS             complement(<1..381)
                     /locus_tag="PLT08_16605"
                     /inference="COORDINATES: protein motif:HMM:NF012868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE06149.1"
                     /translation="MTLLTFFGMIAIPLVASPLVYLSGRLGAHELILHRRSYLVRGLS
                     LLTILLAWIPFVLSAPTLLAGNVQEFDLEAIWLRFDGISLIASGMVLVLGTFVILFSG
                     PYMAGEAGEEKYYAMLLAMIGMMLG"
     gene            complement(382..>1823)
                     /locus_tag="PLT08_16610"
     CDS             complement(382..>1823)
                     /locus_tag="PLT08_16610"
                     /inference="COORDINATES: protein motif:HMM:NF012581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="proton-conducting transporter membrane subunit"
                     /protein_id="HNE06150.1"
                     /translation="QLGRYYFLVLFFIGAMAGLVLSGNLLFTFFFWEITALCSYGLIS
                     FYNDDPKAVAGGIKALIITQVGGVGLLAGTLIAYANLGSFQIADVLAKYNAIPVGALA
                     FMGFSFLVAAAAKSAQFPFHTWLPDAMEAPTPISALIHAATMVNAGIYLLARFYPAFE
                     AIPSWKTSVMLVGVISALITAIMALIATDLKRVLAHSTVSQLGYMVYAIGTGGVLASQ
                     FHLLSHAVFKALLFLTAGTVIHSVGTRDMRRMGGLGKTLPFARNVFIIGVLALIGLPI
                     FNGFWSKELILEAGLEHSPFWAYLLMLIGAGLTAFYTFRMTWLVFFGSARESLHVHAI
                     GSAMKVSLAVLSLGTLTTWLFFGRFSGLLSTSLPFHHIEAESTLEMIQTVSFAPATWF
                     ALLTITLGLTLFFTFGRKIALTENPRWLNALTESSFGFEPINHFVVKLVRNFAEKLRA
                     TQTGELNWNILGIIGALLLVVVVLLLNGA"
BASE COUNT          516 a          429 c          507 g          371 t
ORIGIN      
        1 tcccagcatc atgccgatca ttgccaacag catggcgtaa tatttttctt cgcctgcttc
       61 gcccgccatg tatggaccag agaacagaat aacaaaggtt ccaagtacca agaccatgcc
      121 tgatgcgatt agactgattc cgtcaaacct cagccagatg gcttcgagat cgaattcttg
      181 aacattgcct gcaagaagag ttggcgcaga taagacgaag ggaatccacg ccagcagaat
      241 ggtgaggagg gaaagtccgc gcacgaggta ggagcggcgg tgcagaatca gttcatgggc
      301 acccaaccga ccggaaagat acacaagcgg cgatgccact aacggaattg cgatcatgcc
      361 aaagaaggta agtagagtca tttatgctcc gttcaagagc aatacaacta caacaagcaa
      421 aagagcgccg ataataccga gaatattcca gttcaattcg ccggtttggg tggcgcgtaa
      481 tttttcggcg aaattacgaa caagttttac gacaaaatga ttgataggct caaagccgaa
      541 cgaagattcg gtcaacgcat tcagccagcg tggattttct gtaagggcaa tcttgcgccc
      601 gaaggtgaag aacaaggtca agccgagggt gatggtcaac agggcgaacc aggtggcggg
      661 tgcgaagctg accgtttgga tcatctccaa ggtagattct gcttcaatgt ggtggaaagg
      721 cagggaggtg gataagagtc cactgaatct gccgaagaat aaccaagtgg taagtgtgcc
      781 cagcgataga accgcaagcg agactttcat ggcgctacca attgcgtgaa cgtgcaaact
      841 ttctcgcgca gaaccaaaga agaccagcca tgtcatgcgg aaggtgtaaa acgcggtgag
      901 accagcacca atgagcatga gaagatatgc ccagaacggc gagtgttcca gccccgcttc
      961 gaggatgagt tctttgctcc aaaagccgtt gaagatgggt aagcctatca atgccagtac
     1021 gccgatgatg aagacattac gcgcgaacgg taaagttttt ccaagcccgc ccatgcggcg
     1081 catgtcgcga gtgccaaccg aatgaataac tgtccctgcg gtaaggaaga gcaatgcttt
     1141 gaaaacggcg tgactcaaaa gatgaaattg acttgccaac acgccgcctg tgccaatggc
     1201 atagaccatg taccctagtt ggctgacggt tgaatgtgca agcacacgtt ttaggtccgt
     1261 agctatgagt gccatgatgg ctgtgatgag tgcggagatg acgccaacta acattacgga
     1321 tgttttccaa ctggggatgg cttcgaacgc agggtaaaac cttgccagca aataaatgcc
     1381 cgcattgacc atcgtcgcgg catgaatgag ggcgctgatg ggcgttggcg cttccatcgc
     1441 gtctggcagc caggtgtgga atgggaattg ggcagatttt gccgccgccg cgacgaggaa
     1501 gctaaagccc ataaatgcca atgcgcccac tgggatggcg ttgtattttg cgagcacatc
     1561 tgcaatctgg aaactgccga gattggcata cgcaatgagt gtgcctgcta ataaaccgac
     1621 tccgcccact tgggtgatga tgagtgcctt gatgccgcca gccacggctt tggggtcgtc
     1681 gttgtagaag gagatcagcc cgtatgaaca aagtgcggtg atctcccaaa agaaaaaggt
     1741 aaagagcaga ttcccactta gaaccaagcc tgccattgca ccaatgaaga acaatacgag
     1801 gaagtagtat cgtccaagct ggg
//
LOCUS       DAOQJE010000795         1817 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_55521_length_1817_cov_13.826901, whole genome shotgun
            sequence.
ACCESSION   DAOQJE010000795 DAOQJE010000000
VERSION     DAOQJE010000795.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1817)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1817)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1817
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_55521_length_1817_cov_13.826901"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..453
                     /locus_tag="PLT08_16615"
     CDS             <1..453
                     /locus_tag="PLT08_16615"
                     /inference="COORDINATES: protein motif:HMM:NF039201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNE06151.1"
                     /translation="NNCFWNTKDLYEVLDHYLGIRFIDIENNNHYGWIRCDVKEDGSL
                     LILKDYAYETEPDYPILAGSKTTYQEIEPGNIEGEIYATGNTVHISVPHRPTPEYTVQ
                     IYNMSGQLVVASQYESPFVEIQIEVPRGIYIVRVTSANEQLSKEVLIE"
     gene            complement(562..1449)
                     /locus_tag="PLT08_16620"
                     /pseudo
     CDS             complement(562..1449)
                     /locus_tag="PLT08_16620"
                     /inference="COORDINATES: protein motif:HMM:NF033516.0"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            complement(1497..1763)
                     /locus_tag="PLT08_16625"
     CDS             complement(1497..1763)
                     /locus_tag="PLT08_16625"
                     /inference="COORDINATES: protein motif:HMM:NF013677.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - transposition, DNA-mediated
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HNE06152.1"
                     /translation="MKKSKFTESQIVSILAQQEKGMRVADICREHGISQPTFYQWQKK
                     YSGMQVSELKRMKEMEAELSQYKRIVAELTLQNTVLKDVIEKKL"
BASE COUNT          494 a          363 c          382 g          578 t
ORIGIN      
        1 aacaattgct tttggaatac aaaagatctt tatgaagtgc ttgaccatta ccttggtatt
       61 cgatttattg atattgaaaa taataatcac tatggttgga tcagatgcga tgtaaaagag
      121 gatggcagtt tacttatatt aaaagattat gcgtatgaaa cagaaccaga ttaccccatc
      181 ttagcgggct caaaaacaac ctatcaggaa attgaacctg gaaatattga aggagaaata
      241 tatgctacgg ggaacactgt tcacataagt gttccccacc gtccaacccc tgaatatacc
      301 gttcagattt ataatatgtc aggacaatta gtagtagctt cgcaatatga aagcccattt
      361 gttgaaatac aaatagaggt tccacgcggc atatatatag ttagggtgac ctcagcgaat
      421 gagcaattaa gtaaagaagt tttaatagaa taaatcttag aaccactggt tataacaaaa
      481 gctttgcaaa atggcgggtg aatactacct aagaaaattt aaccacacca ttaattattt
      541 cagactatgt gaaagctccc cttatttagt tgcatttaaa aatagagata attcttgata
      601 ttcatcataa tcgccatcaa aatcttgttg gaatgtggga aagtcctgct ccgggatttg
      661 tgggtggtgg atttttccat atttcaacaa gaaagctctg ggcgtttggt agttgagtgc
      721 ggcatgtggc cgttcattgt tgtagaacca tatccatctg ttggattcag tttgtgcgtc
      781 cgataagtct acgaacaacc acctgtccaa aacgtcttct cgataagtgc ggttaaatct
      841 ttcgatatag ccattttgag aaggtttgcc gggttgaata aaggttaagg tgatattgcg
      901 ttcatctgaa gcgcaccagt cttgtaatgc ctgtgcaata aattcaggac cattatctac
      961 acggatacta ttcggcttgc cgcgccattg aataagttgt tccagctctg cgatcactcg
     1021 ttggctggtt atactttttg ctatggttat attcaaggct tcgcgattgt agtcgtcaat
     1081 gacattaaat gctctgaacg gctggtattg taacaagctg tcacgcataa aatccatgct
     1141 ccattgcaaa ttaggttgca gcggttgtaa taatggttgt tttattcgtt ttgctatgcg
     1201 tttcctgctt ctgcgcttct tactcaatcc aatggcttta tatatgcgat aaacgcgttt
     1261 gtgattctcc ggtctgccca tgttacggag tttgtgatac atcatccaaa atccccaggt
     1321 gggatacttc tcagataggt tacagagcaa ctgtgtaacc tcagcatccc ctggacactt
     1381 taccggctga tatcggtaaa ccgaagcaga gatcctgaac gtttgcaatg cctgccgctg
     1441 tgacatgctg tgtgttgcta ctgcatagtc tacaagctca cgcttttcgg caggcgttac
     1501 agcttttttt ctatgacgtc ctttagaaca gtgttttgca aggtaagctc tgcaactatg
     1561 cgcttatact ggctaagttc agcctccatt tctttcatgc gcttgagttc agagacttgc
     1621 atcccggagt acttcttttg ccattggtaa aaagtgggct ggctgatgcc gtgctcacga
     1681 cagatatctg ccactcgcat gcccttttcc tgttgtgcca aaatagatac aatctggctt
     1741 tctgtaaact ttgatttttt cattttgctt cccgaattta gtagttttaa ttgatactgt
     1801 tttcggggga gccttca
//
LOCUS       DAOQJE010000796         1807 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_56103_length_1807_cov_6.046804, whole genome shotgun sequence.
ACCESSION   DAOQJE010000796 DAOQJE010000000
VERSION     DAOQJE010000796.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1807)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1807)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1807
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_56103_length_1807_cov_6.046804"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..364)
                     /locus_tag="PLT08_16630"
     CDS             complement(<1..364)
                     /locus_tag="PLT08_16630"
                     /inference="COORDINATES: protein motif:HMM:NF013499.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OB-fold nucleic acid binding domain-containing
                     protein"
                     /protein_id="HNE06153.1"
                     /translation="MYRTHLCGELRASHAGQTVTLAGWVNRRRDHGGVAFFDLRDRTG
                     LVQVTINPDLSKEVLDQVASVRNEWVLQIEGVVQKRPEGMANPKMETGEIEVIAKNVT
                     VLNASKTPPFMVNTDADLP"
     gene            770..910
                     /locus_tag="PLT08_16635"
     CDS             770..910
                     /locus_tag="PLT08_16635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE06154.1"
                     /translation="MIVEILVIVAILAVLYGMVSSTEQVLSNREEYVVIDERMTEHRS
                     RL"
     gene            complement(948..>1807)
                     /locus_tag="PLT08_16640"
     CDS             complement(948..>1807)
                     /locus_tag="PLT08_16640"
                     /inference="COORDINATES: protein motif:HMM:NF024852.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HNE06155.1"
                     /translation="LVFDSKNERKIVQGWKHKPEVLWGDIQDERVVESALGGVEAVIH
                     LAAIIPPMTDQNPRLAYGVNVNGTRNLVNAASKQTKRVRFIFTSTYDVYGNTQDQTPP
                     RKVTLPVNPQEEYARHKVEAEKIIQSSNVDWCIFRLASAIPVEMSTKYQSMMFDVPLD
                     DRLEYIHPADAALAICNAVEHAEVWGKVLHLGGGPRCQLRERDIVQGAFETIGIGALP
                     EEYFSTQSYYTDWLDTEESQRLLNYQRYTFADHLESLKKSLGWQIPFIKLFSPLIRYF
                     LVKQSKYQK"
BASE COUNT          402 a          445 c          446 g          514 t
ORIGIN      
        1 cgggcagatc cgcgtcggtg ttgaccatga atgggggagt cttcgaggcg ttgagtacgg
       61 ttacgttctt ggcaatgacc tcgatctcgc ccgtctccat tttagggttc gccatccctt
      121 cgggtcgttt ctgcaccacg ccttcgattt gcaagaccca ctcgttgcgc acgctggcaa
      181 cctggtcgag cacttcctta gaaaggtctg ggttgatggt cacttgcaca agaccggtac
      241 ggtcgcgcag atcaaagaat gccaccccgc cgtggtcacg gcgtcgattc acccagcctg
      301 cgagcgtaac ggtctgcccg gcatggctgg ctcttaattc tccacataaa tgagttctgt
      361 acatacttgc gcctctcttt tgtcattgcg aggggattgc cttgtcgcag agagcgcggt
      421 tcgcaatgac gggtatgatg atgtatataa aaaatcgccc tgcgctttcg cgttgggcga
      481 tttggtttcg acttttttat tcgtcaacca gcaaagcttc gcccaacgat acagggatcg
      541 ttattatcat tattattatc gttatgattg gcgaagaaat taccgttcaa cattggcggc
      601 attatagcac agagttttcc tagtggttca atgggttttg atatctatta attgaagatg
      661 cgtatagtca ttttcgctag tgttaggaaa attttcacaa ttgttaatgg gattccaatc
      721 atcttgcatt atcatcttgc ctgttgaaat taaaagatgg agtaggaaca tgatcgttga
      781 aattcttgta attgtcgcca ttctcgccgt gttgtacggt atggtctctt ctaccgagca
      841 agtactaagc aaccgtgaag aatatgtggt gattgacgaa cgcatgacgg aacaccgctc
      901 gcgcctgtag ttgaaaacag ccccaaattt gattggggct gtttttttta tttttggtat
      961 ttggattgct taacgagaaa atatcgaatc agtgggctaa acaatttgat aaacgggatc
     1021 tgccagccca atgatttttt caaactctcc aaatggtcag caaaggtata acgctgataa
     1081 tttagcagtc tctggctttc ttcggtatca agccagtcgg tgtaatagga ttgggtgctg
     1141 aaatattctt cgggcagggc gccgatgccg atagtttcga atgcgccttg cacaatgtcg
     1201 cgctcacgta gttggcagcg cggaccaccg cccaagtgca agaccttgcc ccacacctcg
     1261 gcatgttcca ccgcattgca gatcgccagc gccgcgtcgg caggatgaat atattccagc
     1321 cggtcatcca gcggcacatc gaacatcatg ctttgatact tggtggacat ctccaccgga
     1381 atcgcagatg ccagccggaa gatgcaccaa tccacattgc tgctttggat gatcttttcc
     1441 gcttcaactt tgtggcgcgc gtactcttcc tgcgggttca ccggcagggt cactttgcgt
     1501 ggtggtgttt ggtcttgcgt attgccgtac acatcatagg tggatgtaaa aatgaagcgc
     1561 acgcgtttcg tctgcttgct tgcggcgttc actaaatttc tagtcccatt gacgttgacc
     1621 ccatacgcca gccgcggatt ttgatcggtc attggcggga tgatcgccgc caaatggatc
     1681 accgcctcaa ccccgcccaa agctgattca accacccgtt catcttgaat gtcgccccag
     1741 agtacttcgg gtttgtgctt ccagccttgt acaattttgc gttcattttt tgagtcgaag
     1801 accaaac
//
LOCUS       DAOQJE010000797         1806 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_56180_length_1806_cov_4.786408, whole genome shotgun sequence.
ACCESSION   DAOQJE010000797 DAOQJE010000000
VERSION     DAOQJE010000797.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1806)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1806)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1806
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_56180_length_1806_cov_4.786408"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..833
                     /locus_tag="PLT08_16645"
     CDS             <1..833
                     /locus_tag="PLT08_16645"
                     /inference="COORDINATES: protein motif:HMM:NF013672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HNE06156.1"
                     /translation="VTLDMEPDHGDPEKKIRLLENLEYFERYVSLINKYNVKLTMFTV
                     TQLFDSHSDLLNKLSARIPLEFSVHSHTHDPHNACSLDEVQNSYAAYKKFQGINPIGY
                     RAPIGRIDKNGLGHLLDHGFEYDASVYPSIRPGEFGYFNLHMPNMPFRVAREDGKSLV
                     EFPFTSIETVRVVFALSYAKLLGWFGYSRLLKLFGLPEVTLLLSHPHDFYFGSIPNKT
                     VGGLEGFAMSRNTGKEFNIFEKMLQTLQEKGYEFVYLSEVYQQVKDSELQTFAWEEWK
                     "
     gene            858..>1806
                     /locus_tag="PLT08_16650"
     CDS             858..>1806
                     /locus_tag="PLT08_16650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE06157.1"
                     /translation="MKSLLNDLKSAIYPIADLRTEESRREHTTRAVFIIIGFCLALST
                     LAVTVLDLIAEKPQYWPIFLTAGLLFLHFLGWLLVQNGRWKEARYLPPALFLIAGSVL
                     TANKELGTIAILQFTLSIMLTAILFETSAQWVSIFISAASYLGIAHFIGEQGSYTERQ
                     LQYINVVVFVAIGVLQQITSGVISLTIKRLTLETETRKKTEESALQKEHILSVIAQAA
                     QLLLDAQNWEDKIDEMLALLGSVSGASHAYLFKNHTDENGRLLTSLKYEWKNPEQDHP
                     SNPDSFQNIPLLEEEMVTWYNLLITGKPFYESTDFFSPEW"
BASE COUNT          522 a          390 c          364 g          530 t
ORIGIN      
        1 gcgttacttt ggacatggaa ccggatcatg gtgatccgga aaagaaaatt cgtctactcg
       61 aaaaccttga atattttgag cgttatgttt cacttattaa caaatacaac gtgaagctga
      121 ccatgttcac ggtcacccag ctttttgatt ctcacagtga cttgttaaac aaactctctg
      181 cccgaattcc attggagttt tccgtccact cccatacgca tgacccacac aatgcctgtt
      241 cgttagatga agtacaaaac tcatatgctg catacaaaaa gtttcaaggt ataaacccaa
      301 tcgggtatcg cgctcctatc gggcgaattg acaaaaatgg tctagggcat ttacttgacc
      361 atggctttga gtacgatgcc agcgtatatc cctccattcg cccaggtgaa tttgggtact
      421 tcaacttgca catgccaaat atgccgtttc gagtcgcccg agaagatggc aagtctcttg
      481 tggagttccc atttacttct attgaaactg tccgcgttgt gtttgctctc agttatgcca
      541 aactcctggg ctggtttggc tattctagat tgctaaaact tttcggtttg cccgaagtta
      601 cacttttact ttcacatcca catgattttt attttggaag cattccgaat aaaacggttg
      661 gagggctgga aggttttgca atgtcgcgca atacagggaa agaatttaat attttcgaaa
      721 aaatgctgca aactcttcaa gaaaaaggtt atgagtttgt ctacttgagc gaagtttatc
      781 aacaagtaaa agatagtgaa ttacaaacct ttgcatggga agagtggaag taatcgaatt
      841 ctcttaagcg ttcgaagatg aaaagtctac tgaacgacct caaatcagca atttatccga
      901 tcgccgattt acggacggaa gaatcacgcc gcgagcacac cactcgggca gtttttatta
      961 tcattggctt ctgccttgct ttatcaacac tcgcagttac agtcctcgat cttattgccg
     1021 aaaaacccca atactggccc atatttctaa ctgcggggtt gttattcctc cactttcttg
     1081 gatggcttct ggtacaaaat gggcgttgga aagaagcgag atatcttccg cctgccttat
     1141 tcctgattgc tggttcagtt cttaccgcaa ataaagaact aggtaccatt gcaatcctac
     1201 aatttaccct cagtattatg ctgaccgcta ttctatttga aacatcagca caatgggtat
     1261 ctatttttat aagcgcagcg tcttatttgg gaatagctca ttttatcggg gaacaaggtt
     1321 catatacaga aagacaattg caatacatca atgtggttgt tttcgtggca attggtgtgt
     1381 tacaacaaat tacatcaggg gttatcagcc taactatcaa acgtttgact ttggaaacag
     1441 agactaggaa aaagacggaa gaatctgcgc ttcaaaaaga acatatactt tctgtcattg
     1501 cacaagctgc ccagctttta ctagatgccc aaaactggga agataaaatt gatgaaatgt
     1561 tggcattatt aggctcagtt tctggcgcca gtcatgcgta tttattcaaa aatcacacag
     1621 atgaaaacgg gcggctatta accagcctaa agtatgagtg gaaaaacccc gagcaggatc
     1681 atccatccaa ccctgactca tttcagaata ttccattgct tgaagaggaa atggtcactt
     1741 ggtataacct ccttattacc ggaaagcctt tttacgaatc tactgacttt ttctcgccag
     1801 aatgga
//
LOCUS       DAOQJE010000798         1806 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_56204_length_1806_cov_2.898915, whole genome shotgun sequence.
ACCESSION   DAOQJE010000798 DAOQJE010000000
VERSION     DAOQJE010000798.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1806)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1806)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1806
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_56204_length_1806_cov_2.898915"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(85..480)
                     /locus_tag="PLT08_16655"
     CDS             complement(85..480)
                     /locus_tag="PLT08_16655"
                     /inference="COORDINATES: protein motif:HMM:NF015651.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase transmembrane
                     domain-containing protein"
                     /protein_id="HNE06158.1"
                     /translation="MLAAILLYLALRGLDWAAFFESLRQANYFYISLIVFWSSSTYFL
                     RAMRWRVLLNYQKTLSPAGVFWANMAGYLGNNILPARVGELIRAAYVSRRETIPVVFV
                     LATGFTERLVDLAALVVIGAASVFFAVTF"
     gene            complement(548..1480)
                     /locus_tag="PLT08_16660"
     CDS             complement(548..1480)
                     /locus_tag="PLT08_16660"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HNE06159.1"
                     /translation="MTEKLISIVTPCFNEEGNVEELYERIKEVMTGFDYQYEHIYIDN
                     ASTDNTSQKLRTLAAQDKRVKVIFNTRNFGHIRSPYYGLLQGSGDAVVIMASDLQDPP
                     ERIPDFIRKWEEGYKAVIGVKTKSRESGIFYLLRTMYYRVLRSLSDVELIEHYTGFGL
                     YDKKVINILKGIGDPYPYFRGLIADIGFPIARIEFEQPLRKRGISKNSFYTLYDIAML
                     GFTNYTKIPLRLAAMFGFLSALVSFLIGLIYLIYKLLFWYQFSLGSAPLVIGLFFLGS
                     VQLLFLGIVGEYIGAIYTQVMHRPLVIEKERLNF"
     gene            complement(1490..>1806)
                     /locus_tag="PLT08_16665"
     CDS             complement(1490..>1806)
                     /locus_tag="PLT08_16665"
                     /inference="COORDINATES: protein motif:HMM:NF020250.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SAF domain-containing protein"
                     /protein_id="HNE06160.1"
                     /translation="KAREMCGAPGTEKVPSSQKEIDYLTSLVRGVYAKHDLPAGHVLK
                     PDDYYLAIPLQKGQLSCRELLNDQVLAKKIKADAPLMVDSMDSLYNRDEGLKKQLARR
                     GL"
BASE COUNT          531 a          425 c          419 g          431 t
ORIGIN      
        1 ttgaaagaat gggagcaaaa aaataaaggc gactccaaca accgcaacaa ttgcgaaact
       61 cttcaacgcc tcctgcatag acgttcaaaa tgtcactgca aagaaaacgg atgcggcgcc
      121 gataacgacc agtgcggcaa ggtccaccaa cctttcggtg aatcctgtgg caagcacgaa
      181 cacaactggg atcgtttccc gtcgggacac ataggcagca cggataagct ctcctacccg
      241 cgcaggtaag atgttattgc caagatagcc cgccatattc gcccaaaaga ctcctgccgg
      301 agaaagcgtt ttttgataat tcagtaaaac ccgccagcgc atggcacgca gaaaataggt
      361 ggaactgctc caaaatacga taaggctgat atagaaataa ttcgcctgac gcaaagattc
      421 gaagaacgcc gcccagtcga gtccgcgcag ggcgagataa agcaaaatgg cggcaagcag
      481 gaatgggatc cagagaagga ggcgattgcg cggtttttgc gatccgggcg agtcggaaag
      541 aagatcccta aaaattgagt cgctccttct cgatcaccaa tggacggtgc attacttggg
      601 tatagatcgc accaatatac tcgcctacaa tgccaaggaa taataattga accgagccga
      661 gaaaaaaaag cccaatcact aacggcgcag aaccaaggga aaattgatac cagaataaaa
      721 gcttatatat caggtatatc aagccgatca aaaaactcac aagagctgaa agaaaaccga
      781 acattgcggc aaggcgcaga gggatcttcg tataatttgt aaaccccagc atggcgatat
      841 catacaaagt ataaaaacta tttttactga tgcctcgttt acggaggggc tgttcaaatt
      901 cgatacgggc aataggaaag ccaatatccg cgatcagccc gcgaaaatat ggataaggat
      961 ccccgattcc ttttaggatg ttaataacct ttttatcgta taagccaaag ccggtgtaat
     1021 gttcgatcaa ttccacatca gagagggatc gcaaaactcg atagtacata gtgcgcaata
     1081 agtaaaaaat acctgattcg cggcttttag tttttacacc gatcacagcc ttgtatccct
     1141 cttcccattt tcgaataaag tcagggatcc tttcaggagg gtcctgcaag tcagatgcca
     1201 taataacaac tgcgtctcct gagccttgca aaagtccata atacggagag cggatatgcc
     1261 caaaattccg tgtattgaaa atgaccttta cccgtttgtc ttgcgcagct agagtgcgaa
     1321 gtttttggga tgtgttgtct gtagaggcgt tatcaatgta aatatgctcg tattgataat
     1381 caaaaccggt catcacttcc ttgatgcgtt cgtagagttc ctccacgttc ccttcttcgt
     1441 tgaaacaagg tgtgacgata ctgatcagct tttctgtcat aactggcacc tacaatcctc
     1501 tgcgggcgag ctgcttcttc agtccttcgt cgcggttata gaggctgtcc attgagtcga
     1561 ccatcaatgg cgcatctgct ttgatcttct tggcaagtac ctggtcattt aacaattctc
     1621 gacaggataa ctgccctttc tgaagcggga tggcaagata ataatcatct ggtttcagta
     1681 catgccctgc gggaagatca tgctttgcgt acacaccgcg cacaagagac gtcaggtagt
     1741 cgatttcctt ttgggaggac ggcacttttt cggtgccggg agcgccgcac atttcgcgtg
     1801 ctttat
//
LOCUS       DAOQJE010000799         1805 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_56235_length_1805_cov_7.202286, whole genome shotgun sequence.
ACCESSION   DAOQJE010000799 DAOQJE010000000
VERSION     DAOQJE010000799.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1805)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1805)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1805
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_56235_length_1805_cov_7.202286"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(117..521)
                     /locus_tag="PLT08_16670"
     CDS             complement(117..521)
                     /locus_tag="PLT08_16670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE06161.1"
                     /translation="MDSSQTADASYQYAENRITSIIKLASGTCETPVLFASKWDFTYD
                     GDGTRTATLITPYNELGEPQSAQLTAYFFGGAYETQADGTIRKYYSLEGQTILRTLTT
                     GSSTLSYFLTDHLGLGRRHRRCQRSNVTSPNS"
     gene            complement(666..1274)
                     /locus_tag="PLT08_16675"
     CDS             complement(666..1274)
                     /locus_tag="PLT08_16675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE06162.1"
                     /translation="MKKTNKKISSKTISILLPLSILIFVVLNVFNVINSTFPHGLGLS
                     DELIRYQSPSAPYSMLVSEKWTILDAPIENYEYTDVRTSIGSPYASLKIYVSRKSFET
                     DTLNDVVDWGRATAGYQLLGRKEEDVSEYSNKKRKGILDEYIIDVSPFIKLTWNSKYN
                     HCFDYYFLEKGYGYKFSFCSVEKVWNNAKDLFIKLIDSIEIG"
     gene            complement(1286..>1805)
                     /locus_tag="PLT08_16680"
     CDS             complement(1286..>1805)
                     /locus_tag="PLT08_16680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE06163.1"
                     /translation="TPTVTGTPTATSTPTIIPTSCIPRPGCTTNTPTPTSTPSSTLTP
                     TYPPTHDAFIFGVSGSFDDGMVSGSSHSAGFEYLYAFDDGSSGLFRTHGYGTSMGESA
                     QAGFYVGRAWGITTAAENVSSSSPSVALTILSAQFTYTGDPWSGSFTVGLGAPGGVSI
                     AYSDNQTVPAFP"
BASE COUNT          467 a          377 c          441 g          520 t
ORIGIN      
        1 gtagtcattt gaacctccaa attcatttga tgttacaggg atatacgcgc gtttttgaat
       61 ttggatgcat cgattcaggg tctgttgacc tgtgtatgag aggtctgcaa ccccgcctat
      121 gagttgggac tggtcacatt gctccgctgg caccggcgat ggcgacgacc gagcccaaga
      181 tgatcggtga ggaaatagga cagggtcgaa gaacccgttg tcagcgtgcg gaggatcgtc
      241 tgcccttcga gcgagtaata cttgcggatt gtaccatcgg cctgggtctc gtatgcgccg
      301 ccgaagaagt aggcggtcaa ctgcgccgat tgcggttcgc cgagttcgtt gtaaggtgtg
      361 atgagcgtgg ctgtgcgtgt gccgtcgccg tcgtaggtga agtcccattt ggaggcgaat
      421 agaacgggag tttcgcaagt tccgctggcg agtttgatga tgctggtgat gcgattctct
      481 gcgtattgat aactggcgtc tgcggtctgg ctgctatcca ccgaggtgac agcgtgaggc
      541 gcggaagtag gggtgcgtag gaggtctact ctatatcatc attaatctct ggtaatgggt
      601 ttgtgtaaat gatgtagcgc gagatattat ctcgcgctac ggacattatc atttattttg
      661 acccatcacc caatctctat actatcaatt aattttataa acaaatcttt cgcgttgttc
      721 catacctttt cgacactaca aaaggaaaat ttataaccat aacccttttc taggaaataa
      781 taatcaaaac aatggttata tttactgttc caagtcaatt ttataaatgg cgaaacatct
      841 ataatgtatt catccagaat tccttttcgt tttttgttcg agtactcaga aacatcttct
      901 tccttacgcc ctaacaattg atacccggct gtcgcccttc cccaatcgac aacatcgttt
      961 aatgtatctg tctcaaatga cttccttgac acgtatattt tcaatgatgc ataaggactt
     1021 cctatgcttg ttcttacgtc agtgtattcg taattttcaa tgggtgcgtc aagaattgtc
     1081 catttttctg ataccagcat tgaatacggg gcactagggc tttggtaccg aattagctca
     1141 tcggataaac caagtccatg agggaaagtt gaatttatta cgttaaacac atttaagact
     1201 acaaatatta agattgacaa gggtagcagt atcgaaatgg tttttgaaga tatttttttg
     1261 tttgtctttt tcatgaagcc gtctcctatg ggaaagctgg cactgtttga ttgtcactat
     1321 aggctataga aactccacct ggggcgccaa ggccaacagt aaaactccct gaccatggat
     1381 cccctgtata agtaaattgc gctgaaagaa tggtgagggc aactgatggc gaagagctgc
     1441 taacattttc agcagcagta gtaatacccc aagctcttcc cacgtagaaa ccggcctgtg
     1501 cggattctcc cattgatgtt ccgtagccat gagttctaaa caaaccagat gagccatcat
     1561 caaatgcata taaatattca aaaccagccg aatgtgagct gccgctaacc ataccgtcat
     1621 caaatgaacc gcttactcca aatatgaagg catcatgagt aggtgggtat gtgggggtaa
     1681 gggtgcttga aggtgtcgaa gtaggtgtag gtgtatttgt tgtacacccg ggacggggta
     1741 tacaactagt cgggataata gtaggagtac ttgttgcagt gggagttccg gtcacagtag
     1801 gggtt
//
LOCUS       DAOQJE010000800         1804 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_56304_length_1804_cov_4.301315, whole genome shotgun sequence.
ACCESSION   DAOQJE010000800 DAOQJE010000000
VERSION     DAOQJE010000800.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1804)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1804)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1804
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_56304_length_1804_cov_4.301315"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..189
                     /locus_tag="PLT08_16685"
     CDS             <1..189
                     /locus_tag="PLT08_16685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE06164.1"
                     /translation="TCVWAGVDSAWEQEKLEAWKMLENAAESHTSGARGVGRRCRCNK
                     ILAATNTKAFLDERKHEI"
     gene            complement(152..643)
                     /locus_tag="PLT08_16690"
     CDS             complement(152..643)
                     /locus_tag="PLT08_16690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE06165.1"
                     /translation="MKRNIFETVIGSIVGGIAFYPLATPLSWIITWSIGDTHHLKNFF
                     LWRWSIFWLILLVGIIIGGILGRYFREKVVLFASFKNVSTSIIFWFTVIFGILGLETG
                     FWVFDSLMPLHTKVPTAIDLLIFSSKFAIVGMVIGSWISFLVILIRLAIIKFHAFFRP
                     KTP"
     gene            complement(847..1638)
                     /locus_tag="PLT08_16695"
     CDS             complement(847..1638)
                     /locus_tag="PLT08_16695"
                     /inference="COORDINATES: protein motif:HMM:NF013695.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HNE06166.1"
                     /translation="MNKTTFVIQKMDCASEEQLIRLKLGDFNNISSLQFDIPNRKLDV
                     YHTGDYKPIFSEIHNLQLDASFIQTSATNEIFTGKDIAERKLLWQVLAINFFFFIFEI
                     LMGFISNSMGLVADSLDMLADSLVYGLALFAIGGTIAKKKNIAKASGYFQLILAVFGV
                     VEVIRRFLGFGEVPTFQTMIIISSLALLGNATSLYLLQKSKNTEAHMQASMIFTSNDV
                     IINIGVIIAGVLVFVTNSKIPDLVVGAIVFFIVARGAYRILQIAK"
BASE COUNT          589 a          370 c          358 g          487 t
ORIGIN      
        1 acctgcgtgt gggcgggcgt ggattctgct tgggagcagg aaaaactcga agcgtggaaa
       61 atgctcgaaa atgccgcaga atcccacacg tcaggtgcac gcggtgttgg gcgcaggtgt
      121 agatgcaaca aaattctggc agccactaat actaaggcgt ttttggacga aagaaagcat
      181 gaaatttaat tatggcaaga cgtatcaata taaccaaaaa actaatccat gaaccgatta
      241 ccattccaac aattgcgaat ttacttgaaa atattaatag gtcaatcgcc gtgggtactt
      301 tagtatgcaa aggcattaaa ctgtcaaaaa cccaaaagcc agtttccagc ccaagtatac
      361 cgaatatgac agtaaaccaa aaaataattg aagtagatac gtttttgaat gaggcgaata
      421 aaaccacctt ctctcggaaa tacctaccaa ggattccccc aataattatg ccaacaagca
      481 gaatgagcca gaatatactc caccgccata aaaagaaatt ttttaagtga tgagtatctc
      541 ctatgctcca tgtaataatc caagaaagag gtgtagcaag tgggtaaaaa gcaattcctc
      601 caactattga accaattact gtttcaaaaa tatttcgttt catctaaatg gacaacctta
      661 tcgttgaagt cactttcggg ctgaattgat acttaagcgc ccaacggctt gcgttacctg
      721 cgtgtgggcg ggcgtggatt ctgtttggga gcaggaaaaa gcccgaagcc agaaaaatgc
      781 ttgaaaatgc cgcagaatcc cacacgtcag gtgcacgctt tgttagccac cgtttttgtt
      841 aacagactac tttgcaattt gcaatatgcg atatgcgcca cgcgcaacaa taaaaaatac
      901 aattgctccg acaaccaaat cagggatttt ggaatttgtt acaaagacta atacgcccgc
      961 aataatcaca ccaatattga ttattacatc gttagaagta aaaatcatac ttgcttgcat
     1021 gtgggcttcg gtatttttac ttttttgaag caaatacagg ctggttgcgt ttccaagtaa
     1081 agcaagtgac gaaattataa tcattgtttg aaaggtgggg acttcaccaa aaccaagaaa
     1141 acgcctgatt acttcgacaa cgccaaagac agccagaatt aattggaagt aaccgctggc
     1201 tttggcaata ttttttttct tggcaattgt tccaccaatg gcaaataacg caagcccata
     1261 aacaagacta tctgccagca tgtccaaact atctgcaaca agccccattg aattggaaat
     1321 aaatcccatc aggatttcaa agataaagaa gaaaaagttg atggctagaa cttgccaaag
     1381 caattttcgt tcagcaatat ctttgcctgt gaaaatttca ttcgtcgcgc tggtttgaat
     1441 aaaagatgcg tcaagttgta agttgtgaat ttcgctaaaa atcggcttgt aatcacctgt
     1501 atgataaaca tctaatttgc ggttaggaat atcaaactgc aaactagaaa tgttattgaa
     1561 gtctccaagt ttgagacgga ttaactgttc ttccgatgca cagtccattt tttgaatgac
     1621 aaacgttgtt ttattcaagg ctaattcctt attttagaga gacggaaaac gaatcaatgt
     1681 ttccgtctct cttgtcgtga taatcttatg gcttattttc cagcgacaac cccgcttctt
     1741 cccaagaggt tgtaccgccg tccaacatcc aaaggttagt atagccctga tttagtaaag
     1801 tggc
//
LOCUS       DAOQJE010000801         1803 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_56356_length_1803_cov_4.033753, whole genome shotgun sequence.
ACCESSION   DAOQJE010000801 DAOQJE010000000
VERSION     DAOQJE010000801.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1803)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1803)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1803
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_56356_length_1803_cov_4.033753"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1803)
                     /locus_tag="PLT08_16700"
     CDS             complement(<1..>1803)
                     /locus_tag="PLT08_16700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE06167.1"
                     /translation="ALLKVDEVYTFYEDSKGAIHIAHRKPSVYLFQDSIVRKKMYFDA
                     LVQGDGINSYVKIGNETFAIGNRIIINARLKQKDSTVYTILTPPPNPYLGVATSGNHL
                     LFSLANGLILFKWENNKLTKINELIGDLTGSVFSDKLGRFWVCSQKYGAVCFDNKNKD
                     FSNPRVYLDKKKITSMTQDQQGTYWFCTIGEGIYTLTVNHADSFNERDGLISKNITAI
                     NIDYNNRVWTGDDEGIVHMQKHNNLFTHWLIGPNDGYNRCRQIVPLADNTCWIVSDDY
                     LKWGDGVTMHKSITHAAYKSALVKNNQIWYVSYSHLGYIDLNEKNFGNRIKEIRFTTI
                     GLDINGYVWAGHMEGIYNLHDNFSYNWGNSFPLLKSRIIAIQAADSNHLWVATPTSGL
                     LWVQIHDGTVQKVDIVNERIKKPIENIQSMYREDNGRLWLATNHGVYRLSPDWSVLHL
                     DHTNGLAADDVNAVVVKGDTLWAGTVAGLSRLVLSTLGGDGEFATMISSLRFTLDNQV
                     FTYHLLDSATTRRFTTLTADARIIELDLSALDFTCRGKINFEYIIRQELLPWYFMTPN
                     NLFQWLLNGFSFPSDTTFLEKGTLNLGVQLEPGR"
BASE COUNT          539 a          360 c          336 g          568 t
ORIGIN      
        1 acctgcccgg ctcgagttga acacccagat tgagcgttcc tttttccaga aaagttgtat
       61 cggaagggaa agaaaaacca tttagcagcc attgaaataa attattggga gtcataaagt
      121 accagggtag taattcttgc ctgataatat attcgaagtt gatcttgcct cggcaggtaa
      181 aatccagcgc tgacaaatcc agttctataa tcctggcatc tgcggtcaag gttgtaaaac
      241 gcctcgtggt agcagagtcc aacagatgat atgtgaatac ttggttgtcc agggtgaaac
      301 gcagtgaaga gatcatcgtt gcaaactctc catcgccgcc gagtgtagag agtaccaatc
      361 tgctcaaccc ggccacagtc cctgcccata aagtgtcacc tttaacaaca acagcattga
      421 cgtcgtcagc ggccaaacca tttgtgtgat caagatgtaa tactgaccag tcagggctta
      481 acctataaac accatggttg gtagccagcc aaagcctacc gttatcttcc cgatacattg
      541 actggatatt ttcaattggt tttttgattc gctcattgac aatatccact ttctgaactg
      601 ttccatcatg tatttgtacc cacagcaatc cggaagtggg tgtagcaacc caaaggtggt
      661 tggagtcggc agcctgaata gcaatgatgc gagatttaag tagtggaaat gagttgcccc
      721 agttataaga aaaattgtcg tgtaagttat aaattccttc catgtgacca gcccaaacat
      781 aaccatttat atcaagacca atcgtagtaa accgtatttc tttaatcctg tttccaaaat
      841 ttttttcgtt taaatcaata tatccaaggt gagagtatga aacataccag atctgattat
      901 tttttactaa cgctgactta tatgctgcgt gagtaattga tttatgcatt gttacgccat
      961 cgccccattt taagtaatcg tcggaaacaa tccaacaagt attgtctgct aatgggacaa
     1021 tctgtctgca gcgattataa ccatcatttg gaccaattaa ccaatgtgta aaaaggttgt
     1081 tgtgtttttg catatgtaca ataccctcat catcaccagt ccatactcta ttattgtaat
     1141 caatgtttat tgcagtaata tttttagaaa taagcccatc cctttcatta aaggaatccg
     1201 cgtgattaac agttagagta taaatgcctt caccgattgt gcaaaaccaa tatgtaccct
     1261 gttgatcttg ggtcattgat gttatttttt tcttgtcaag ataaacgcgg gggttactaa
     1321 agtctttatt tttattgtca aaacatactg cgccgtattt ttgagaacat acccagaatc
     1381 ttcctaattt gtcagaaaaa acggatccag ttagatcccc tattaattca tttattttag
     1441 taagtttgtt gttctcccat ttaaagagaa tcaatccatt tgcgagggaa aatagtagat
     1501 gattgcctga agttgctact ccaagatatg ggttaggggg aggggtcaat atcgtatata
     1561 cagtgctatc tttttgtttt aatctagcat taataataat tctgtttccg attgcgaagg
     1621 tttcattgcc gatttttaca tacgaattaa tgccgtcacc ttgaactaat gcatcaaaat
     1681 acatcttctt tctaacaatg gaatcttgaa aaagatacac agacggcttt ctatgagcta
     1741 tatgtattgc accttttgaa tcttcataaa atgtatagac ctcatctact tttaataatg
     1801 cta
//
LOCUS       DAOQJE010000802         1801 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_56470_length_1801_cov_5.382589, whole genome shotgun sequence.
ACCESSION   DAOQJE010000802 DAOQJE010000000
VERSION     DAOQJE010000802.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1801)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1801)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1801
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_56470_length_1801_cov_5.382589"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(165..>1801)
                     /locus_tag="PLT08_16705"
     CDS             complement(165..>1801)
                     /locus_tag="PLT08_16705"
                     /inference="COORDINATES: protein motif:HMM:NF021127.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="CapA family protein"
                     /protein_id="HNE06168.1"
                     /translation="ILFSACAPATEAPASPAETTVPAQPTSTQAPVADALWISPATPD
                     VLREAATNSGVPIVNDAESATQKLDVADSGSLWIYALVAPFPTVTDGISSADLISAWQ
                     NGSSLTGNGQPLLMAESTLRAFTALWGEPASGTVSIVASDQLLTTAWTQPFSWAIIPF
                     EEIQPKWKVLSVDGQSPIHKDFDANLYPLKLSFGLTNSNIELPATNRDASKLATVVLT
                     GVTALVRATAFTMENKGITRPGELVYNWLHDADVLHISNEVPFDPDCPYPTPGYKNFI
                     LCSDPKYMELLTYVGTDIVELTGDHFADRGTQAMLDTLQMYRDNNIPYYGGGANEEEG
                     RKPVLMNVNGNKIAFMGCNGKVSYVFVKATDTKPGAADCDYEFFTQQVKDVAAQGYMV
                     IFTFQHEECYHHGPCYKHEEGFHAVADAGATIVSGSQAHYPHLMEFRGSTFIHFGLGN
                     LFFDQMTYELPDGSVIDETRREFIDRHVFYDGKYLGTELLTAMLEDFSRPRPMNERER
                     AQFLSEYFAYSGWTELQPTPIPQPTVTLTPIILPTP"
BASE COUNT          463 a          428 c          459 g          451 t
ORIGIN      
        1 actgccaaca ccactcttga acttatattt ttcatcgaaa cctccttcag gttacgatga
       61 aattctagat gccagcggtt agaagagtat ggcggggatg taaaaaggtt gtaaatttta
      121 taaagagctc cgaggttttg aaaacctcgg agctcttggt tcgcctatgg agttggcaaa
      181 ataatcggcg tcaatgtgac agttggctgt ggaatgggcg tgggctgcaa ttctgtccag
      241 ccgctgtagg caaagtattc agaaaggaat tgcgcccgtt cacgttcgtt catcgggcgc
      301 ggacgggaga agtcttcgag catggcggtc agcaattcag tgccgagata tttgccatcg
      361 tagaacacat gacggtcaat aaattcgcgg cgggtttcat caatgaccga accgtccggc
      421 aattcatacg tcatttgatc gaagaacaaa ttgcccaaac caaaatggat aaaagtactg
      481 ccgcggaatt ccatcaaatg cgggtaatgc gcctggctgc cactcacaat cgtagcgcca
      541 gcatccgcta cggcgtgaaa accttcttca tgcttgtaac aaggtccatg gtgataacac
      601 tcttcatgct ggaaagtaaa gataaccata tagccttgcg ccgctacatc tttgacttgt
      661 tgcgtgaaga attcataatc acagtctgca gcgccgggtt tggtatcggt cgctttcaca
      721 aagacatacg aaacttttcc gttacagccc ataaacgcaa ttttgtttcc attcacattc
      781 attaggaccg gtttgcgccc ctcctcttca ttcgcgccgc cgccataata tggaatattg
      841 ttatcacgat acatctgcaa ggtatccaac atggcttgcg tgccgcggtc tgcaaagtga
      901 tcaccggtta gttccacaat gtcagtgcct acataggtaa gcaattccat atatttcgga
      961 tcactgcaca aaatgaaatt tttatagccc ggggttgggt atgggcaatc gggatcaaaa
     1021 ggtacttcgt tgctgatatg caacacatct gcatcatgca accagttata gacgagttcg
     1081 ccgggacgag ttatcccttt gttttccatc gtgaatgccg tagcacgcac aagtgcggtc
     1141 acaccggtca acaccacagt tgccaatttt gaagcatcac gattcgtagc aggcaattca
     1201 atgtttgagt tcgtcagccc gaacgaaagt ttcaacggat acaagttcgc atcaaaatct
     1261 ttatggatcg gcgattgacc gtctacgctt aagactttcc actttggttg gatttcttca
     1321 aaggggatga tcgcccaact aaaaggctgt gtccacgctg tggttaatag ctggtcagat
     1381 gcaacgatgc tcactgtgcc cgaagcgggt tcgccccaca gggcagtgaa cgccctcaga
     1441 gtggattctg ccatgagcaa cggctgacca ttcccggtta aggaagagcc gttctgccag
     1501 gcgctgatca ggtctgcgga cgagatacca tctgtcaccg tagggaaagg tgcgacgagg
     1561 gcataaatcc aaagagagcc tgaatcggca acatctagtt tttgagtggc gctttcagca
     1621 tcattcacga ttggcacgcc agaatttgtg gcagcttcac gcaagacgtc aggcgtggcg
     1681 gggctaatcc acaacgcatc tgcaacgggt gcttgtgtgg aggttggttg agcagggacg
     1741 gttgtttcag cgggcgaggc gggtgcttca gtagcaggcg cacaagcaga gaatagtatt
     1801 g
//
LOCUS       DAOQJE010000803         1799 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_56614_length_1799_cov_4.174885, whole genome shotgun sequence.
ACCESSION   DAOQJE010000803 DAOQJE010000000
VERSION     DAOQJE010000803.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1799)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1799)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1799
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_56614_length_1799_cov_4.174885"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..572)
                     /locus_tag="PLT08_16710"
     CDS             complement(<1..572)
                     /locus_tag="PLT08_16710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE06169.1"
                     /translation="MATAKRKASQDRRRSFGERFDLNTVSLTGPVIAVWGRNGDVFVR
                     IQSPNLLSLPGPQSYVNIVIPGGMIDGDPVTLQKGDLLRVTGYVRQSNFQESLRLILE
                     GAGKRTFFDRSEILQEDRLAWKRIYIKRRNSIVQALKVEVLVAPAESQNFVDLEGIVS
                     EAWLYPRDDHEDLFFRIAIYDEHTIETANEG"
     gene            complement(811..1641)
                     /locus_tag="PLT08_16715"
     CDS             complement(811..1641)
                     /locus_tag="PLT08_16715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE06170.1"
                     /translation="MLANKLMTLAGIPWSTVEVELDKELPADAYSPVPGGASLTDIDP
                     AFMRDEFNRLFGVCGFGWGYEYDEDNVVLDTVKRGQGTAFSAIIKKLIFWVKLLDDEG
                     KEVILKIASSGGSVNDVQGYALSGAITNAIGKAASNIGFQKSVYLGARSHKNVGAKSP
                     KPAATSAAPPKATQAVKKPAAPAKPAAQAPKPQPKIQEPIDDEIIDDEPAAAVSEASN
                     FVISFGNRKGQKLGDVDLKYIAWYANDMKTEGDPEKVALQNAAKELLKIRSNGHAVPA
                     "
BASE COUNT          437 a          466 c          438 g          458 t
ORIGIN      
        1 ccttcattgg cagtttcaat agtgtgttca tcgtaaatcg caatacggaa aaatagatct
       61 tcgtgatcat cacgcggata caaccatgct tcgcttacaa tgccctccag gtcaacaaag
      121 ttttgcgact cggcaggagc aactaaaact tcaaccttga gagcttgaac gattgaatta
      181 cggcgtttaa tgtaaattct cttccacgcc aaacgatctt cctgaagtat ttctgatcgg
      241 tcaaagaaag ttcgcttccc tgcaccttct aggatgagac gcaaggattc ctgaaaattc
      301 gactggcgga catacccagt gacacgcaga agatcgccct tttgcagagt caccgggtcg
      361 ccgtcaatca ttccgccagg aatgactatg ttcacataac tttgtggacc aggcagagac
      421 agcaggtttg gggattggat gcgaacaaac acatcgccgt tgcgtcccca aactgctata
      481 accggacctg tcagagatac cgtatttagg tcaaaacgct caccgaacga cctacgtcga
      541 tcctgagatg cttttctttt ggctgttgcc atataaacct ccaaattaat tatcaggagc
      601 agacgagcat gtccctgtag taaatgatct tattcggtgc atactccctt gtacccctgg
      661 gtatgctcca aatagcatag gttcgcatgt aatggcgaac cacttgtttc tgaattgtta
      721 atgtgcgact gcggaaacag gcaaatgtgt gcctgataac ctgcgtattt gggagatgga
      781 gttgccatct cccaaacaga gccttgcggt ttatgccgga actgcgtgac cgttggagcg
      841 aatctttagc aactctttgg cagcgttttg caacgcgacc ttctcgggat caccttccgt
      901 cttcatgtcg ttggcatacc aggcgatata tttcagatcc acatcgccga gcttctgtcc
      961 tttgcggttt ccgaaggaga tcacaaagtt ggaagcttca gatacggcag cggcaggttc
     1021 atcatcgatg atctcatcgt cgatcggttc ctgtatcttt ggctgaggct tgggagcctg
     1081 tgccgcaggt ttggcaggag ctgctggctt tttgacggct tgcgtcgcct ttgggggtgc
     1141 ggcagaagtg gctgcaggtt tcggggactt cgcacccaca ttcttatggg aacgcgcccc
     1201 aagatatacg gacttttgaa aaccgatgtt cgatgccgct ttcccaatgg cattggtgat
     1261 cgcacccgag agcgcgtacc cttgaacatc attcaccgag ccgccagatg aagcgatctt
     1321 caaaatcact tccttgcctt catcatcgag aagcttgacc cagaagatca atttcttgat
     1381 gatggcagag aaagcggttc cttgaccacg ctttaccgta tcaagcacaa cattgtcttc
     1441 gtcgtactcg tacccccacc cgaaaccgca cacgccaaaa aggcggttga attcatcgcg
     1501 cataaatgcg ggatcgatgt ccgtcaaact tgcaccgccc ggaacaggcg aatacgcatc
     1561 ggctgggagt tccttatcca gttccacttc cacggtggac caggggatgc ccgcaagggt
     1621 catcagtttg ttagctaaca ttggaatacc tccatagatt gaaatagcgc gtcccggcgt
     1681 tgatccacca ggacgcaaga aacgtcttgg tgaacccgta cacggatcgc gccggaggaa
     1741 ttccttttcc ggcatattca cttgtaaagg ttctgtcagg ctgggtagcc tgcgaagat
//
LOCUS       DAOQJE010000804         1796 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_56810_length_1796_cov_6.859276, whole genome shotgun sequence.
ACCESSION   DAOQJE010000804 DAOQJE010000000
VERSION     DAOQJE010000804.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1796)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1796)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1796
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_56810_length_1796_cov_6.859276"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1035
                     /locus_tag="PLT08_16720"
     CDS             <1..1035
                     /locus_tag="PLT08_16720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE06171.1"
                     /translation="FDLLISGVDFAGKVLSAIISRPDQPKNPEPQKIESKEITTTTTF
                     EKEIHHHHEPQVIEREIRTEPKIIEKEIHHKPTIIENSEPKIIEKEIHHEPITIYEPV
                     KIVEKRVLDKKFLTDVVDKTVEKLSILLNKQTVQFFYILEEHQIRGAIQELQARVSAL
                     KTLVNYTTLDPNIATQIMVSSLNPLQVSLNIVEIRLKDAGQNELWEYCYVVGNSALLT
                     GYAYLGQDLPALREELEDHLKIIQNKLINQVAREIIISGGEIPWNEVPSMLKPENAYK
                     ILDLNKSYQQSLQMPFDLNKMSYDRVKASLRKVKNISMLKRIYTEEEKNRKRVRVLKA
                     IEQRKDELDK"
     gene            1199..1702
                     /locus_tag="PLT08_16725"
     CDS             1199..1702
                     /locus_tag="PLT08_16725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE06172.1"
                     /translation="MKFISTVASLFIVFGCCILSAIFELPERYIERDVKVEEMVGTWN
                     LTSDSEADVNTWINYHWDWGIDAPWKTFTLNKDGSCKVEFQSEWLSSSDLATTNVISC
                     TWKLEKEENLSDKISPVLKFKFEYSDKGTSMFSSYLFEENNKMIVWNFIGDPDDFITQ
                     DFVKVKQ"
BASE COUNT          642 a          327 c          346 g          481 t
ORIGIN      
        1 tttgatttat tgatttcagg agttgatttt gctgggaaag ttttaagtgc cataatttct
       61 cgtcctgacc agcccaaaaa tcctgaaccg caaaaaatag agtcgaaaga aataacaacg
      121 accactactt ttgagaaaga aatacaccat caccacgagc cacaggtgat tgaaagagag
      181 attcgcactg agccaaaaat aattgaaaaa gaaattcacc ataagcctac gattattgaa
      241 aacagcgagc caaagataat cgagaaagaa atccatcacg aaccaataac aatttatgag
      301 ccagtaaaaa ttgttgaaaa gcgagtgctg gacaagaaat ttttaacaga tgtagtggat
      361 aaaactgtag aaaaactatc tattctatta aataagcaaa cagttcaatt tttttacatt
      421 ttagaagaac atcaaattcg cggcgcaatt caagaattgc aagccagagt aagcgcattg
      481 aaaacgttgg taaattacac aacgttagac ccgaacattg ctactcaaat tatggtatct
      541 tcattaaacc ctctccaagt cagtttgaac attgtagaga ttcgattgaa agacgctgga
      601 caaaatgaac tttgggaata ttgctatgta gttggcaata gcgctctgct tactggatac
      661 gcctatttag gacaggacct tcctgcctta agagaagaat tggaagacca cttaaaaata
      721 atccaaaaca aattaatcaa tcaagtagca cgagaaataa ttatcagcgg tggagaaatc
      781 ccctggaatg aagtaccgtc tatgttaaaa cctgaaaatg cctataaaat ccttgacctt
      841 aataagtcct atcagcaaag tttacaaatg ccttttgact taaataaaat gtcttacgac
      901 agagtgaaag cgtctttacg aaaagtaaaa aatattagta tgttgaagcg aatctataca
      961 gaagaagaga aaaacagaaa acgagtgaga gttttgaagg caatcgaaca acgcaaggat
     1021 gaactcgata aataaaaaac tgcccaacaa agcgtgcacc tgacgctggg gattctgcgc
     1081 acatctcaag cagttttaca cgccttatca tttttctggc tggacggctt cgccgtcccc
     1141 accccagcac aggtaacgca agccgttggg cagttcagca agaaggttgt tttacatcat
     1201 gaaatttatt tcaacagttg cttcattatt tattgtcttt ggctgttgta ttttgagtgc
     1261 aatttttgaa cttcctgaaa gatacatcga gcgtgatgtg aaagtggaag aaatggtagg
     1321 cacatggaat ttaacatctg atagtgaagc agatgttaac acatggataa actatcattg
     1381 ggactggggt attgatgcgc cgtggaaaac ctttaccctt aataaagatg gtagttgcaa
     1441 agtagaattt caatctgaat ggttaagttc atctgattta gcaacaacta atgtaatctc
     1501 atgtacttgg aaattagaga aagaggaaaa tctaagcgat aaaatatctc ccgttcttaa
     1561 gttcaaattc gaatactccg acaaaggcac atcaatgttc tcgtcttacc tctttgaaga
     1621 aaacaacaaa atgattgtat ggaattttat tggcgaccct gacgacttta ttacgcaaga
     1681 ctttgttaaa gtaaaacaat gaagaaattt tcaagatttc atatttggaa aatcactgcc
     1741 caacaaagcg tgcacctgac gctggggatt ctgcggcaat cccaagcagt tttcta
//
LOCUS       DAOQJE010000805         1796 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_56814_length_1796_cov_4.449167, whole genome shotgun sequence.
ACCESSION   DAOQJE010000805 DAOQJE010000000
VERSION     DAOQJE010000805.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1796)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1796)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1796
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_56814_length_1796_cov_4.449167"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..879)
                     /locus_tag="PLT08_16730"
     CDS             complement(<1..879)
                     /locus_tag="PLT08_16730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE06173.1"
                     /translation="MEEIAQTTRGENIIPPLLDRTWKRVLFGLFVVTAPIFNFSLIEL
                     MKPEWQDGRFTSYLYLFLLPEASWGFFPLLLYAILSYLLLLWDSEQFADSMIIRLGIY
                     GGTLLALQYSILSFIATFSPSPVVLAVILAYSAPLLLPKLYFWLRPQLDKLFKRYRLA
                     MIIILLIIIALVLWGFFFVLAIFGIASPFWSFLVAFQASRWLLKHYETKFSFTKGLGV
                     VAWISAYAFALRFNIIKMFELYNALPTEPPNCYIATAAAKGHPRFVGSREVTLASGKS
                     MRVNRQLQRLKALEIVW"
     gene            complement(892..1716)
                     /gene="rsmI"
                     /locus_tag="PLT08_16735"
     CDS             complement(892..1716)
                     /gene="rsmI"
                     /locus_tag="PLT08_16735"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0000451 - rRNA 2'-O-methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HNE06174.1"
                     /translation="MGTLYLVATPIGNLEDMSPRAVRILREAILIAAEDTRHTGTLLK
                     HFNVETPLTSYFEHNKLTKQDFILEKLAQGDVALVSDAGTPAINDPGYELVQAALAAS
                     FDVRPVPGPSAPISALTVSGLPTDSFLYLGYLPHKSSERHNRLNEVSEQPYTLIFFET
                     PHRIADSLEDIRSTLGNRRICVAREMTKMYEEFWRGDVSGAIEYFNSKEPRGEFTLVI
                     EGKKKEERTVWTETELTEAIKRELQEGRSAKEISAELAEKSGWNKKEVYALVNQVK"
BASE COUNT          513 a          391 c          445 g          447 t
ORIGIN      
        1 ccaaacaatt tccaacgctt tgagcctctg caactgacga ttgacccgca tggattttcc
       61 gcttgccaat gtcacctctc gcgaaccgac gaagcgtgga tgtcccttcg cggcggcggt
      121 ggcgatgtaa caattgggcg gttcggtagg caaggcgtta tacaactcga acattttgat
      181 gatgttgaag cgcagggcaa aggcatacgc tgaaatccaa gcgacgacgc caagtccttt
      241 tgtgaaagaa aacttggttt cgtagtgttt caaaagccaa cgagaagctt ggaaggcaac
      301 aaggaaagac cagaagggtg aagcgatgcc aaagatcgct aatacaaaga aaaatcccca
      361 cagaacaagg gctatgatga tcaatagaat gatgatcata gcaagccgat accttttgaa
      421 caatttatcc aattgaggtc tcaaccaaaa gtacagcttt ggtagtagga gaggtgcaga
      481 atatgcaagg ataacagcta aaacaacagg agatgggcta aatgtagcaa tgaatgacaa
      541 aatgctgtat tgcagagcaa gcagtgtgcc gccatagatg ccaaggcgga tgatcattga
      601 atcagcgaat tgttcactat cccatagtag caagagataa ctgagaattg catacaaaag
      661 taaagggaag aatccccaag aagcctctgg caaaaggaag agatagagat aggatgtaaa
      721 ccgaccatcc tgccactctg gtttcatcag ttcaatcagc gaaaaattaa agatcggggc
      781 agtaacaaca aaaagcccaa agagaacacg cttccaagtc ctatctaaca atggcggaat
      841 aatattttcc cctctggtcg tttgagctat ttcttccatg catggctcct attacttgac
      901 ctgattcacc aaggcgtaca cttctttttt attccaaccg cttttttctg ccaattctgc
      961 agagatctct tttgcagatc ttccttcttg aagttcgcgt ttgatcgctt ctgtgagttc
     1021 ggtttcagtc caaacggttc tttcttcttt cttctttcct tcaatgacca gagtaaattc
     1081 acccctaggt tctttgctgt tgaaatattc gatcgcccca ctcacatctc cgcgccaaaa
     1141 ttcttcgtac atcttcgtca tctcgcgggc tacgcagatg cgacgattgc cgagggtaga
     1201 gcgaatatcc tcgagcgagt ctgcgatgcg gtgaggagtt tcgaaaaaga taagagtata
     1261 aggctgttcg ctgacttcgt tgagtcggtt atgtcgttcg ctggatttgt ggggaaggta
     1321 gccaaggtac aaaaatgaat cagtggggag accagaaacg gtcaaggcgg agatgggagc
     1381 tgagggaccg ggaacaggtc gaacgtcgaa agaagcggcg agggcggctt gaacgagctc
     1441 atagccaggg tcattaatgg cgggcgtccc tgcatcggaa acgagggcta cgtcgccttg
     1501 tgctaatttt tcaagaatga aatcttgctt ggtgagttta ttatgttcga aataactggt
     1561 gagaggtgtt tcaacattga agtgcttgag caaggtgccg gtgtgacgtg tatcttcagc
     1621 ggcaatcaga attgcttcgc gaagaatccg cactgcgcgc ggactcatat cttcaagatt
     1681 tccaattgga gtggcgacaa gatataaagt tcccatgcaa ataattatct cacgaaataa
     1741 aaatcgggcg attgctcgcc cgattttgtt tatccgccgc agcccttaag ctggat
//
LOCUS       DAOQJE010000806         1792 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_57022_length_1792_cov_6.152562, whole genome shotgun sequence.
ACCESSION   DAOQJE010000806 DAOQJE010000000
VERSION     DAOQJE010000806.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1792)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1792)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1792
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_57022_length_1792_cov_6.152562"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(49..>1792)
                     /locus_tag="PLT08_16740"
     CDS             complement(49..>1792)
                     /locus_tag="PLT08_16740"
                     /inference="COORDINATES: protein motif:HMM:NF014442.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TaqI-like C-terminal specificity
                     domain-containing protein"
                     /protein_id="HNE06175.1"
                     /translation="GGVFYTPTYIVDYIVENTVGKLVEKKSPKDVVSLKIVDPACGSG
                     TFLLGAYQFLLDWHLNWYVENEPEKAEKSKSIVKVGDGYRLTTTKKKEILLNNIHGVD
                     IDAQAVEVTKLSLLLKVLENASGQLGLGMERVLPDLGNNIKCGNSLIGYDFFEGHLFP
                     DEAERTRINPFDWRSEFQGVFNKGGFDVVIGNPPYIQLSMADYYNESVSSYFGNRFSS
                     SMGRLNTFGLFIEKVLHSVLAKKGLFSFIVPNTLLTQEYYQGLRKQILKYQVKSITNY
                     KYPVFKDAVVETIVFTICNLDPDKHQVEVVDFDNREMASAKHLISQEIFSSTHNNAFL
                     VTSDEKSFDFKQSLDKKGVSLGELANLNQAIALKHDRSKSLFTVAKAKNYKPVIDGRN
                     INRYKLVWDGQYLAYDVANIHSCKRTDIFEAGEKLFFRRVGDRLIATYDDQQFYALNT
                     LVVITPQEQVKFSLKYLLGLINSSLLNFYYVKFLKSTKKVFSEIQARQLAQLPIHIIN
                     FSNSIESSQHDKIVSFVEQMLELNKRLLKAKTPQEIESLERQIKITDKAIDSLVYELY
                     GLNEEEIKIVEGSL"
BASE COUNT          542 a          366 c          299 g          585 t
ORIGIN      
        1 gtgcacgctt tgttggcaag ccttttgact tggaagactc tttgcctatc acagagaacc
       61 ttccacaatt ttgatttctt cttcattcaa cccgtataac tcataaacga gtgaatcaat
      121 tgccttgtct gttatcttaa tttggcgttc caaactttct atttcttgcg gggttttcgc
      181 cttcaaaagc cttttattta attctagcat ttgttcaaca aacgacacaa ttttatcgtg
      241 ttgggatgat tcgattgaat tggaaaaatt gattatgtga ataggaagtt gcgctaattg
      301 cctagcctga atttcagaaa atactttctt tgtggatttc aagaatttca cataatagaa
      361 attcaacaaa ctagaattta tcaagcccaa taagtatttg agagaaaatt tcacttgttc
      421 ttgcggcgta attacaacca aagtattcaa ggcgtagaat tgttggtcgt cataagttgc
      481 aattaatcta tctccaactc gcctgaaaaa taatttttct cctgcttcaa aaatatcagt
      541 ccgtttacaa gaatgaatat tggctacatc gtaagccaaa tattgaccat cccaaaccaa
      601 cttatatcta ttgatatttc gtccgtcaat aacaggcttg taatttttcg cctttgcaac
      661 cgtaaataaa gatttgcttc tatcgtgctt taaggcaatg gcttgattca aattcgccaa
      721 ttccccaagt gatacacctt ttttgtctag gctctgctta aagtcaaaag atttttcatc
      781 agatgtaacc agaaaggcgt tattgtgcgt agacgaaaaa atttcctgtg aaattaagtg
      841 ctttgcagaa gccatttctc tattgtcaaa atcaacaact tctacttggt gtttatctgg
      901 gtctaaatta cagattgtaa aaacaattgt ttcaacaact gcgtctttga acacggggta
      961 tttatagttc gttatgcttt tgacttggta cttcaagatt tgctttctca agccttgata
     1021 gtattcttgt gtaagtaaag tgtttggaac aatgaaacta aaaagacctt tctttgccaa
     1081 aacagaatgt aaaacttttt cgatgaacaa gccaaaggta ttcaatcgtc ccattgacga
     1141 agagaagcga tttccgaaat atgaactaac cgactcgttg taatagtccg ccattgaaag
     1201 ttgaatataa ggcggatttc caatcactac atcaaagcca cctttattaa atactccttg
     1261 aaattcactc cgccaatcaa aaggatttat tcgagttcgc tctgcttcat cgggaaataa
     1321 atgaccttca aaaaaatcgt agccaattaa agagtttcca cacttgatat tatttcccaa
     1381 gtcgggcaaa actcgctcca tgcccaaacc taattgaccg ctggcatttt ccagcacttt
     1441 gagcaaaaga gaaagtttgg tcacttctac tgcttgtgcg tcaatgtcca caccatgaat
     1501 attattgagc aagatttctt ttttcttcgt cgtagttaac ctgtaaccat cgccaacttt
     1561 tacaatggat ttgcttttct cggctttttc aggctcattt tcaacgtacc aattcaaatg
     1621 ccaatccagt aaaaactgat atgcgccaag caggaaagta cccgaaccgc aagcagggtc
     1681 aacgattttg agcgaaacaa catcttttgg acttttcttt tcaacaagtt ttccaaccgt
     1741 gttttctacg atgtaatcca caatataagt aggggtgtag aatacaccgc ct
//
LOCUS       DAOQJE010000807         1791 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_57093_length_1791_cov_5.033986, whole genome shotgun sequence.
ACCESSION   DAOQJE010000807 DAOQJE010000000
VERSION     DAOQJE010000807.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1791)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1791)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1791
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_57093_length_1791_cov_5.033986"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..983
                     /locus_tag="PLT08_16745"
     CDS             <1..983
                     /locus_tag="PLT08_16745"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNE06176.1"
                     /translation="SVISISKLAKHYQVPEREAGLKAAAKGLFKRTYKTVKAVDGISF
                     DIQPGEVVGFLGPNGAGKTTTLKMLSGLLHPTSGNLSVLGYEPAKRNHDYLRQITLVM
                     GNRNQLSWDLPALDSFDLQRAIYGIPTDEFKRTRDEFIELLDLKELVQKPVRNLSLGE
                     RMKMEIVGALLHKPKILFLDEPTLGLDVTMQKRIRSFIAEYNKRYGATVLLTSHYMAD
                     VEALCKRVIVIHHGQLLFDGDLAALVQKFSAFKTVAFDIDNPNHDFSQYGDVVSIDGL
                     HVTLRVPKEKTSAVTTRLLSDLAVDDLTVEATPIEDVIESVFSVKKEALE"
     gene            980..1783
                     /locus_tag="PLT08_16750"
     CDS             980..1783
                     /locus_tag="PLT08_16750"
                     /inference="COORDINATES: protein motif:HMM:NF017946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-2 family transporter protein"
                     /protein_id="HNE06177.1"
                     /translation="MKRYFDFYFTSMKMAVIEQWQYPIANYFYMIGMIAEPVIYMVVW
                     SAVATQQGGTVGGYTPGAFAAYYIVWTLVRSMNVVFTPYGWEWRIRQGQLSMSLMRPI
                     HPLHNDVAFFAGWKVVVIILWIPLALALTLFFKPDLHPTLLNGIVFFFAIWFAYLIRT
                     MTLSLLGMVTFWTTRVSALFELYFAAELVLSGRLVPLSLMPEWVQKLAWFFPFRWAFG
                     YPIEALIGNMPVNDLFIGLGMQLAWILFGVMAVSLVWKVAIRRFSAVGG"
BASE COUNT          396 a          444 c          441 g          510 t
ORIGIN      
        1 cgtctgtcat ttcgatctcg aaactggcaa aacattacca agtccccgaa cgtgaagcgg
       61 gcttaaaagc cgccgcgaaa gggctcttca aacgcaccta caagaccgtc aaagctgtag
      121 atggcatctc ttttgatatt caacccggag aagtggtcgg ttttctcggt ccgaatgggg
      181 caggaaaaac caccacactc aaaatgctca gcggactttt gcaccccaca tctgggaatt
      241 tatcggtgtt ggggtatgag cctgctaaac gcaaccacga ttatttacgc caaatcacgt
      301 tagtgatggg aaaccgcaat caactctctt gggatttgcc cgcccttgat tcatttgatt
      361 tgcaacgtgc catttatggc attccaactg atgagttcaa acgcactcgt gatgaattca
      421 tcgaattgtt agacttgaaa gaattggtac agaagcctgt tcgcaatctc tctttaggtg
      481 aacgcatgaa gatggaaatt gtcggcgcat tactacacaa gccaaaaatc ctcttcctcg
      541 atgagccgac gctgggtctc gatgtgacca tgcagaagcg catccgttct ttcatcgcgg
      601 agtataacaa acgctacggc gcgacggtgc tgctcaccag tcactacatg gcggatgtgg
      661 aagccctgtg caagcgcgtc atcgtcatcc accacggaca gttgcttttt gatggtgatc
      721 ttgccgcgtt agtccaaaaa ttctccgcct ttaaaacggt cgcctttgat attgataatc
      781 ccaaccatga tttcagccaa tatggtgatg tagtctccat tgatggttta catgtgacct
      841 tgcgcgttcc taaagagaaa acctccgccg tcacaacccg tctgcttagc gacctcgctg
      901 tagatgatct taccgtcgaa gccacgccta tcgaagatgt gatcgaatcc gtcttttcgg
      961 tcaagaaaga ggcgctggaa tgaagcgata ttttgacttc tatttcacat ccatgaagat
     1021 ggctgtcatc gaacagtggc agtatcccat tgccaactat ttctatatga tcggtatgat
     1081 tgctgagccg gttatttata tggtggtctg gtctgcggtg gcaacccagc aaggcggcac
     1141 agtgggcgga tatacccctg gcgcgttcgc tgcctactac attgtgtgga ctctcgtccg
     1201 tagcatgaat gtggttttca cgccctacgg gtgggaatgg cgtattcggc aaggtcaact
     1261 gtcgatgagt ctgatgcgcc ctattcatcc gcttcacaac gatgtggcat tcttcgctgg
     1321 ttggaaagtc gtcgtcatta tcttgtggat tccgctggca ttggcgctca ccttgttctt
     1381 caagcctgac ttgcatccga ccttgctaaa tggcatcgta ttcttttttg cgatttggtt
     1441 tgcgtacctg attcgcacca tgaccctctc tctgcttggc atggtcactt tttggacgac
     1501 acgtgtcagc gccttgttcg aattgtattt tgccgctgag ttggtccttt caggtcgcct
     1561 tgttccgctt tctttaatgc cagagtgggt gcaaaaatta gcatggttct tcccattccg
     1621 ttgggcattt ggttatccca ttgaggcgct gatcgggaat atgcctgtca atgacctgtt
     1681 cattggcttg ggtatgcagc ttgcgtggat cctctttggc gtgatggctg taagcctggt
     1741 gtggaaagtt gccatccgcc gcttttcggc agtaggaggt taatatgacc g
//
LOCUS       DAOQJE010000808         1791 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_57094_length_1791_cov_4.700461, whole genome shotgun sequence.
ACCESSION   DAOQJE010000808 DAOQJE010000000
VERSION     DAOQJE010000808.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1791)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1791)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1791
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_57094_length_1791_cov_4.700461"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..511
                     /locus_tag="PLT08_16755"
     CDS             <1..511
                     /locus_tag="PLT08_16755"
                     /inference="COORDINATES: protein motif:HMM:NF013324.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HNE06178.1"
                     /translation="QARGVIRDVASQYGVEEIVSSKRAEMEQSIDDQLVAIFAENNLI
                     MRDFVLRNIRFSDEYAAAVEQKQIAEQQAQQAKFVVEQKKQEAEQARQVAQGSADAAV
                     IAAKGEAEALLIQAQAQAEANQVIAQSLTPELVQYQYVQKLSPNVQTIFIPSGNQFIL
                     PLPSSPQGQ"
     gene            569..1627
                     /gene="selD"
                     /locus_tag="PLT08_16760"
     CDS             569..1627
                     /gene="selD"
                     /locus_tag="PLT08_16760"
                     /EC_number="2.7.9.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012871322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004756 - selenide, water dikinase
                     activity [Evidence IEA];
                     GO_process: GO:0001887 - selenium compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_except=(pos:629..631,aa:Sec)
                     /transl_table=11
                     /product="selenide, water dikinase SelD"
                     /protein_id="HNE06179.1"
                     /translation="MSIPQSGREVRLTTLSACAGUASKLSADALAQVLRPVKDIFDPA
                     AYPDLMVGLESPDDAAIWRLSDDQAIVVTTDFFTPVVDTPYEYGAIAAANSLSDVYAM
                     GGRPFLALNIAALPDHLPAEISGEILRGGAEKAREAGVVIAGGHTVKDKEPKYGLVVI
                     GFVHPQKVISKGGLKAGDVLVLTKPLGAGVTTTALKQEKADEAHVLEAIEWMARLNKN
                     AGMLASEFGVRGGTDITGFGLLGHASEMAEASHVALHFDYSKIPFLSGARGYAERGIF
                     PGGAFDNKSHFEALVKFSAEISEPAQMLLFDPQTSGGLLFGLAKEKLSAFEARAKETN
                     QPYWVIGEAREGEGIEVR"
     gene            1629..1775
                     /locus_tag="PLT08_16765"
     CDS             1629..1775
                     /locus_tag="PLT08_16765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE06180.1"
                     /translation="MFRIGVLELGVTCAFVALTIIIPLIIARGYAQINRRLKNIENTI
                     AKKK"
BASE COUNT          462 a          395 c          493 g          441 t
ORIGIN      
        1 gcaagctcgt ggcgtgattc gtgatgtggc atcccaatat ggcgtagaag aaattgtcag
       61 ctcgaagcgc gctgaaatgg aacagtcaat cgatgatcaa ttagtagcta tctttgctga
      121 aaacaaccta attatgcgcg attttgtttt gcgcaacatc cgcttcagtg atgaatacgc
      181 cgcagcggtt gagcagaagc aaatcgctga gcaacaggca caacaagcaa aatttgtagt
      241 ggagcagaag aagcaggaag ctgaacaggc tcgtcaggtg gcgcaaggtt ctgcagatgc
      301 cgccgtgatc gcagcgaaag gtgaggctga ggcgttgttg attcaggctc aggcgcaggc
      361 agaggcgaac caagtcatcg ctcaatcact cacgccggaa ctggttcaat atcagtacgt
      421 gcagaagctt tcgccgaacg ttcagacgat cttcatccca agcggcaatc aatttattct
      481 gccgttgccc agttcacccc aagggcagta atcgagtaca ctacagacct gacggttccc
      541 gtcaggtctg taatctataa aggagcatat gtccattcca caaagcggac gtgaggtacg
      601 cctcacaact ctttcagctt gtgctggttg agcgtctaaa ctaagcgccg atgcgctggc
      661 gcaggttctg cgccctgtca aagatatctt tgaccctgct gcatatcccg acttgatggt
      721 gggtcttgag tcaccggatg atgcggcaat ttggcgttta agtgacgatc aagcgattgt
      781 ggtaacgaca gatttcttca ctcccgttgt agacactcct tatgaatacg gcgcgattgc
      841 cgcagcaaat agcctctcag atgtctatgc catgggcgga cgtccgttct tggcgttgaa
      901 tattgcggca ttgccagatc acctaccggc ggagatttca ggcgaaattt tgcgtggtgg
      961 tgccgagaaa gcccgtgaag cgggagtcgt tattgcaggt gggcatacgg tcaaagacaa
     1021 agagccaaaa tacggcttgg tggtgatcgg gtttgtgcat ccacaaaaag tgattagcaa
     1081 gggcggatta aaagccggcg atgtacttgt gcttaccaaa ccgctcggcg ctggagtcac
     1141 cacgacggcg ctaaaacaag aaaaagcaga tgaggctcat gttcttgaag ccattgaatg
     1201 gatggcgcgt ttgaacaaaa atgccgggat gcttgcttct gaatttggcg tccgcggcgg
     1261 gacggatatc actggcttcg gtttgctggg tcatgcctca gagatggcag aagcatctca
     1321 tgtggcattg cattttgatt attcgaaaat cccatttttg agtggcgcac gtggttatgc
     1381 tgagcgtgga attttccctg gtggagcgtt tgataacaaa agtcattttg aagctctggt
     1441 gaagttctct gctgaaataa gtgaacctgc tcaaatgctt ttgttcgacc cgcaaaccag
     1501 cggcggatta ttgtttggtt tggcaaaaga aaaactgtct gcttttgaag cgcgtgcaaa
     1561 ggaaacaaac caaccgtatt gggtaattgg tgaagcacgc gaaggggaag gcatcgaggt
     1621 gcgttaacat gtttcgcatt ggtgtgctgg agttgggtgt cacgtgcgcg ttcgttgcct
     1681 tgacgatcat cattccgtta atcattgccc gtggatatgc acaaatcaac aggcggttga
     1741 agaatattga aaatactatc gcaaaaaaga agtaactttc aggtacaata a
//
LOCUS       DAOQJE010000809         1781 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_57776_length_1781_cov_4.097335, whole genome shotgun sequence.
ACCESSION   DAOQJE010000809 DAOQJE010000000
VERSION     DAOQJE010000809.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1781)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1781)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1781
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_57776_length_1781_cov_4.097335"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..255
                     /locus_tag="PLT08_16770"
     CDS             <1..255
                     /locus_tag="PLT08_16770"
                     /inference="COORDINATES: protein motif:HMM:NF024769.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdeI/OmpD-associated family protein"
                     /protein_id="HNE06181.1"
                     /translation="GDMADFEIAYDPIKRAFPIHPKFEKALKENKQAKEVFDSLRPSL
                     QLEINRYFSFLKTEESVDRNVNRAIQFLLGKSRFVGRDKP"
     gene            475..1020
                     /locus_tag="PLT08_16775"
     CDS             475..1020
                     /locus_tag="PLT08_16775"
                     /inference="COORDINATES: protein motif:HMM:NF024698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNE06182.1"
                     /translation="MKNKKKDDVNIRLWSDNDFLLLERLMGDSAMTEHLGGPETAEKI
                     RERHERYCQSSVSGNDPMFVIILEPEGIASGSIGYWKREWQGKTIWETGWSILPEFQG
                     YGIATKATALIVERARMESKHRFLHAFPSIDNAPSNAICRKAGFVLCEEVNFEYPPGS
                     ILRCNDWCLDLFAHDSTVLPA"
     gene            1112..1582
                     /locus_tag="PLT08_16780"
     CDS             1112..1582
                     /locus_tag="PLT08_16780"
                     /inference="COORDINATES: protein motif:HMM:NF012422.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HNE06183.1"
                     /translation="MTQITQLSEREKEVVKYLLQGKSNKLIAVSLGISDRTVEFHLKN
                     IYIKYQVNSRVELILKLGYSTVDNLGENVENRGKLNSQINWATPFKETISIIGKESNM
                     KNKWMFQIANSILWAAAIIASAIVNAPSFLSSILLPVLAVGSLIVMESKLNNSV"
BASE COUNT          538 a          356 c          395 g          492 t
ORIGIN      
        1 ggcgacatgg ctgattttga aattgcttac gacccaataa aacgagcttt cccaatacat
       61 cctaaatttg aaaaggctct aaaggaaaac aaacaagcaa aagaagtttt cgactctttg
      121 cgcccttctt tacaattaga aattaaccga tatttcagtt ttttgaaaac agaagaatcc
      181 gttgacagaa atgtaaatag ggcgattcag tttctattag gaaaaagtag atttgttggc
      241 agggataagc catagccaat gacaaaaata ccttcccacc aatgcctccc gtcaaagcat
      301 gcacctgatg ctggggctgc cgtaaggcat gcgcaaatct cagtcagttt tctgcgcctt
      361 ggcctttttc tggttggacg gctcaactgt ccagccctca gtgcaagtaa cgtaaaccgt
      421 tagcctgctc cttgcgtaat aggtgtttgg tgtaatcgga acttggagag ttatatgaaa
      481 aataaaaaga aagacgatgt caatattcgt ctatggtcag acaatgattt tttacttctt
      541 gagagattga tgggcgattc tgccatgacc gagcacctcg gcggacccga gactgcagaa
      601 aagattcgtg aacgtcatga gagatattgt cagagcagtg tttctggcaa cgatcccatg
      661 tttgtgatta ttcttgaacc tgagggaata gcatctggct caattggata ctggaagaga
      721 gagtggcagg ggaaaactat atgggaaact ggctggagta ttcttccaga gtttcaagga
      781 tatggaatcg ccacaaaggc aacggctttg atagttgagc gtgctcgcat ggaaagtaag
      841 catcgttttc tccatgcttt tccgtctatt gataatgcac catcaaatgc gatttgtcga
      901 aaggcaggtt ttgtgctttg cgaagaagtc aattttgaat acccgccagg tagtattttg
      961 cgttgcaatg attggtgttt agatttattt gctcacgatt cgacagtgtt gcctgcctga
     1021 tgtttacaca aaatcgaaag cgggcccatt gcaggtaacg ccaactgttg acttgcccct
     1081 catgcaacaa atcaaaggaa acaaattcgc catgacacaa attactcaat tgagtgaacg
     1141 tgaaaaagaa gtagttaaat accttctgca aggaaaaagt aataaactga tcgccgtgtc
     1201 acttggtatt tctgaccgaa cagtggaatt ccatctgaaa aatatttaca taaaatatca
     1261 ggtgaactcc cgagtagagt taattttgaa actagggtat tccacagttg ataatctggg
     1321 ggaaaacgtc gaaaataggg gcaagctcaa ttcgcaaata aattgggcta cacccttcaa
     1381 agagaccatc tctataatcg gcaaggagtc aaatatgaaa aataaatgga tgtttcaaat
     1441 cgcaaattca attctctggg cggccgcgat tattgcatcc gcaatagtca acgcaccttc
     1501 attcctttca agcattctat tgcctgtgtt ggcggtaggg tctttaattg tgatggagtc
     1561 taaacttaat aatagtgtgt aggtttactt ttctgtaaca ctagctctct gcaaatttaa
     1621 atactttaga aataggaaat gtaaaagcaa gctaacaaag cgcacgtctg acgttgagga
     1681 ctgccgtaag acgtgcggta attcccaatc ttttcctgcg cccgaacatt tttccagtcg
     1741 gacggattcg ccaaccaagt aacgcaaccc gttgggcatt t
//
LOCUS       DAOQJE010000810         1781 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_57785_length_1781_cov_3.665122, whole genome shotgun sequence.
ACCESSION   DAOQJE010000810 DAOQJE010000000
VERSION     DAOQJE010000810.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1781)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1781)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1781
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_57785_length_1781_cov_3.665122"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(117..704)
                     /locus_tag="PLT08_16785"
     CDS             complement(117..704)
                     /locus_tag="PLT08_16785"
                     /inference="COORDINATES: protein motif:HMM:NF020757.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009036 - type II site-specific
                     deoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BglII/BstYI family type II restriction
                     endonuclease"
                     /protein_id="HNE06184.1"
                     /translation="MKIVATYDFNGGKSAVLQRYAAEFAQIQSVISAIDASLYKTKES
                     TEKTMMGKMLFSPTELNKAFKREFAKAENGGWSNHKVLCDYSYGEYSEDYIKSQNTRG
                     QPFRDMDFVKPNTKLGIEVQFGKYAFMVYNVCAKMTIFSKMGVIDTGIEIVPVKNFAD
                     EMSTGVSYFEQFAWDLEHRGTSNIDVPVLILGIDA"
     gene            complement(701..1651)
                     /locus_tag="PLT08_16790"
     CDS             complement(701..1651)
                     /locus_tag="PLT08_16790"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013704.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HNE06185.1"
                     /translation="MIDKHSISAEFSADEYIVVTESDTLEALKKLPASSFKLAVSSPP
                     YNIGKEYEKQIELQHYLDWQSEILKELARVISDDGSIVWQVGNFVDDGEVFPLDIYFY
                     PIIKSLGFQLRNRIVWHFDHGLHATKRFSGRYEVLLWFTKSKDYTFNLDSVRVPSKYP
                     GKLHYKGDKKGQPSGNPLGKNPSDYWSIIAQEWESGIIEIPNVKSNHPEKTSHPCQFP
                     IELIERCVLALTNENDWVLDPFGGVGSSLIAAIKNGRRGMVIDRDSQYIAITKERISA
                     FQEGVLKIRPLGKPVHKPTGKEKVAQMPSEWLNEKQEKLL"
BASE COUNT          438 a          384 c          370 g          589 t
ORIGIN      
        1 gcgcgatgca tcacggtggg gaaagcgttt gacttttggg gagaagccct tgtagttgga
       61 gttaagcggc gcgctttagc gcgtccgctt gaacgacttg ttatgccttt ttagtttcaa
      121 gcatcaattc caagtattaa tacggggaca tctatgttcg aagtgcctct atgctccaaa
      181 tcccaagcaa actgttcaaa ataggagacc ccggtactca tttcatcggc aaagtttttg
      241 acaggaacga tttctatgcc ggtgtcaata acgcccattt tgctaaatat ggtcattttg
      301 gcgcaaacgt tgtaaaccat aaaagcatac ttgccaaatt gaacttcaat gccaagcttt
      361 gtatttggct ttacaaagtc catgtctctg aatggctgac ctcgtgtatt ttgagatttt
      421 atgtaatctt ctgaatactc tccgtaggag taatcacaca atactttatg attcgaccat
      481 cccccgtttt ctgctttggc aaactcacgt ttgaacgctt tatttaattc tgttggtgaa
      541 aatagcattt tccccatcat cgttttttct gtgctctctt tggtcttgta gagacttgca
      601 tcaatcgcac ttataaccga ttgaatttgt gcaaactctg cggcgtacct ttgaagtact
      661 gctgattttc cgccgttaaa atcatatgtg gctacgattt tcaaagtaat ttctcctgct
      721 tttcattgag ccattctgat ggcatttgcg cgactttttc ttttccagtt ggcttgtgca
      781 ctggttttcc aaggggtcta atttttagaa caccttcttg aaatgcggaa atcctttctt
      841 tggtaatggc tatatattgt gaatcacgat caatcaccat tcctcttcgc ccgtttttta
      901 tggctgcaat taagcttgag ccaactccgc caaatggatc caaaacccag tcattctcat
      961 tggtgagtgc aagaacgcag cgctcgatta gctcgattgg gaattggcat ggatgagagg
     1021 ttttttctgg gtgatttgat ttaacatttg gaatttcaat tattccactt tcccattcct
     1081 gagcaattat tgaccaataa tctgatggat ttttaccgag cgggttgccg cttggctgac
     1141 cttttttgtc gcccttgtag tgcagtttgc ctggatattt agagggaact cttaccgaat
     1201 caaggttaaa cgtgtaatcc tttgattttg taaaccacaa cagcacttcg tagcgtccag
     1261 aaaatctttt cgttgcgtgc aatccgtggt cgaaatgcca aacaattcga tttctcaact
     1321 gaaagccaag actctttatg attggataga aatagatatc tagcgggaaa acttctccat
     1381 catcaacgaa atttccaact tgccaaacga tgctgccatc gtctgaaatg actcgtgcaa
     1441 gttcttttag aatctctgat tgccagtcaa ggtaatgttg tagctcaatt tgcttctcgt
     1501 actctttccc aatattgtac ggtggtgaag aaactgccag cttgaatgat gaggctggta
     1561 gtttttttag tgcctctaat gtgtcgcttt ctgtgacaac aatatactca tcagcgctaa
     1621 attcggcgga aatactgtgt ttatctatca tctttttagc ccaataccat gtttggcgta
     1681 gtttatcctt agcggctctg cgagtgaagc tggggtttgg gttgtaaggc ataacgaata
     1741 gcgtttatct gccgcgccga aagcaccagg tccagccgca a
//
LOCUS       DAOQJE010000811         1779 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_57905_length_1779_cov_8.645592, whole genome shotgun sequence.
ACCESSION   DAOQJE010000811 DAOQJE010000000
VERSION     DAOQJE010000811.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1779)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1779)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1779
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_57905_length_1779_cov_8.645592"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1290
                     /locus_tag="PLT08_16795"
     CDS             <1..1290
                     /locus_tag="PLT08_16795"
                     /inference="COORDINATES: protein motif:HMM:NF028237.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase N-terminal 7TM
                     domain-containing protein"
                     /protein_id="HNE06186.1"
                     /translation="STALLGLIFSVIIWGFAYAMELASVSLKWQIFWAKVEYLGIPFV
                     PLFFFIFAYQFTQSTSKLSRRVIFFLVVISSIFVLLAWTNEFHNLIWTELGQKNIDGY
                     YMLTLGHGMAFWLLITYSYILLAIASISIIRRALSGPAEIRKQTYIILVGASVTWLGN
                     IIYISGLSPVPELDLTPIGFVISSVIFSFGIFGYGMLDIMPIAGEFILESLDDVVIVT
                     NPKGMLIYVNRAFEYYFSNPVQSLIGRQATEAFSNLPVLKSLFDSSNTIRREITVTPE
                     NRPLFIFNVRTFNIRLSANEVIGRVTILNDITERRAAENRLSQQSLEAGSGDLPLMVM
                     YRVSDDKIVDVNRTFLINLGYERKDVLGRTLMGIQFWGVSQRADFLRMLYKNGSLKDY
                     AFEIGKNGQKYILSASQIEMQDTKYIVIFAQEDWSGS"
     gene            complement(1363..>1779)
                     /locus_tag="PLT08_16800"
     CDS             complement(1363..>1779)
                     /locus_tag="PLT08_16800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE06187.1"
                     /translation="TIKERYPQLNIKFAPKDWEALLIILRQRSFETAQNIDRFQADSI
                     MLRNIAFGLLLMTLANVIAFFTTMNVFYLLLAFAELFACWMLASESFKFRTWFFTQIY
                     EGSLIYGTSIEEVISHGKGQVSKSKGQKQTSTKIKL"
BASE COUNT          465 a          419 c          383 g          512 t
ORIGIN      
        1 tcgacagcgc tcctcgggtt gatcttttcc gtcatcattt ggggatttgc ctacgccatg
       61 gagcttgcca gtgtcagcct gaaatggcag atattctggg caaaagtgga ataccttggc
      121 attccttttg tgcctttatt ctttttcata tttgcttatc aattcacgca aagcaccagc
      181 aaattatcgc gccgcgttat tttctttctg gtcgttattt cctccatatt cgtccttctg
      241 gcatggacta acgaattcca caatttgatc tggaccgaac tcggtcaaaa aaatatcgat
      301 ggctattaca tgctgacgct tgggcatggc atggcgttct ggcttctaat cacctactcg
      361 tatattctat tggctattgc ttcgatcagc atcattcgta gagccttaag cggacccgct
      421 gaaattagga agcaaaccta tatcattttg gtcggcgcaa gcgtcacatg gttggggaat
      481 attatttaca tttccggttt gagcccggtg cccgaattag acttaacccc gatcggcttc
      541 gtcatctcca gtgtcatctt cagcttcggc atctttgggt acggcatgct agacattatg
      601 cccatcgccg gagagttcat tctcgaaagc ttggatgatg tagttatcgt caccaacccc
      661 aagggaatgt tgatctatgt caaccgtgcc tttgaatatt acttcagcaa ccccgtacaa
      721 tccctgatcg gcaggcaagc taccgaagct ttttccaact tacccgttct gaaatctcta
      781 tttgatagct caaataccat tcgcagagaa attacggtca cgcctgaaaa tcgtccgctg
      841 ttcatcttca atgtgcggac gtttaacatc cgcctcagcg ccaacgaggt gattgggcgt
      901 gtgaccattt tgaacgatat taccgaacgc cgcgccgcag aaaaccggct ttcacaacaa
      961 agcctggaag cagggtctgg ggatttgccc ttgatggtga tgtatcgcgt cagtgacgat
     1021 aagatcgtgg atgtgaaccg caccttctta atcaacctcg gatacgaacg caaagatgtt
     1081 ctgggacgca ctttgatggg cattcaattc tggggtgtaa gccaacgagc cgacttttta
     1141 cgcatgctgt ataagaacgg ctctctcaaa gattatgcat ttgagatcgg gaagaacgga
     1201 caaaaatata tcctttctgc cagccagatc gaaatgcaag atacgaagta cattgtgatc
     1261 ttcgctcagg aagattggtc tgggtcgtaa tttcccactt aaagaccaag aggcaatttg
     1321 gtgataccaa attgcctctt ttttattcct ttatccaata aactacagct ttattttggt
     1381 actggtttgt ttttgaccct tactttttga aacttgccct ttcccatgcg agatgacctc
     1441 ttcgatactc gtgccataga tcaaagaacc ttcatagatt tgagtaaaga accaagtgcg
     1501 gaacttaaag ctctcagaag ccaacatcca gcaagcaaag agctcagcaa aagcaagcag
     1561 caaataaaat acgttcatgg ttgtaaagaa agcgatcacg ttcgcaagcg tcattagcaa
     1621 aagcccaaag gcaatgttcc tcaacataat gctgtcggct tgaaaacggt caatgttttg
     1681 tgcagtttca aaactgcgtt ggcgaagaat gatcaacagc gcttcccaat cttttggcgc
     1741 aaacttaata ttaagttggg gatatctttc tttgatcgt
//
LOCUS       DAOQJE010000812         1779 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_57916_length_1779_cov_3.961137, whole genome shotgun sequence.
ACCESSION   DAOQJE010000812 DAOQJE010000000
VERSION     DAOQJE010000812.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1779)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1779)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1779
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_57916_length_1779_cov_3.961137"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1343
                     /locus_tag="PLT08_16805"
     CDS             <1..1343
                     /locus_tag="PLT08_16805"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNE06188.1"
                     /translation="YTSLYISFNVLRNLQCVTTFFKEREIHLNLILFTASYPYVRGNE
                     ASFLNIEVEYLSKVFDRVLVVPETIKESTPVNHPPVEVNTDYAEMLASKKISEISRSA
                     FTSHILPKGIMELGFPLFSPTAWRRIIAFSGKAELTRRWVDDFLKHHHYVPKDSLFYT
                     YWLDHAVAGIGMLREKYPELALISRAHGYDIYEEQYYDPPFWPCRQTVLNSMDKVYAD
                     SYAGSDYLRRRYSSFLDKFAVSLLGVRDPGFLNLPSEDNVFRIVSCSRISPEKRVDLL
                     LEGIASAARMRPNQKFEWHHFGNGDQREFLQQQADTSFPSNAKAFLPGFSDGNALMDY
                     YRNNPIDVFVNCSSTEGTSVAIMEAMSCGIPILATSVGGNVEIVSEKNGLLIPATVTS
                     FEIAAGLLAFVDDPEPSAKRQGSRQKWSVEYNADVNFPEFAHDLKLIRLGKADK"
     gene            1354..>1779
                     /locus_tag="PLT08_16810"
     CDS             1354..>1779
                     /locus_tag="PLT08_16810"
                     /inference="COORDINATES: protein motif:HMM:NF024912.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE06189.1"
                     /translation="MCGIFGVWNVQKHPLDLQAMLDSVHAIRHRGPDDEGFLLIDTEA
                     HDAVPCSGVDSATQLGLRRIEDCYQPRFDLFFGYRRLSIIDVSPAGHQPMSNIDGSLW
                     IVFNGEIYNYIELRSELQSLGYVFRTQTDTEVILNAYHAW"
BASE COUNT          466 a          358 c          394 g          561 t
ORIGIN      
        1 cttacacctc gctttacatt tcttttaatg tactaagaaa tttgcaatgc gtaacgacct
       61 tctttaaaga aagagagata cacttgaatt taatcttatt tacagcttct tatccttatg
      121 tgagagggaa tgaagccagc tttttaaata ttgaagttga atatttatcg aaggttttcg
      181 accgtgtttt agttgtgccg gaaactataa aggaatctac accagttaac catccacctg
      241 ttgaagtaaa tactgattat gctgaaatgt tagcatccaa gaaaatctcg gaaatatcac
      301 ggtctgcatt tacttcgcat attttgccaa aaggcatcat ggagttggga ttccctcttt
      361 tttcgcctac tgcctggaga cggataattg ccttctcagg taaggcagaa ttaacccggc
      421 gatgggttga tgattttcta aaacatcacc attacgtacc gaaagatagt ctgttctata
      481 cttattggct tgaccacgcc gtggcaggta ttggaatgct tagagaaaaa tatcctgagc
      541 ttgcattgat ctcgcgcgca catggttacg atatttatga agaacagtat tacgaccctc
      601 cattttggcc ctgtcggcaa actgtcttga atagtatgga taaagtatat gcggattcat
      661 atgcaggatc agactatttg agaaggcgct attcgagttt tttggataaa tttgcagttt
      721 ctttgttggg cgtacgcgac cctggttttt tgaacctccc ttccgaagat aatgtctttc
      781 gcattgtttc ctgttcacgc atcagtcccg aaaaacgggt ggatctctta ctggaaggga
      841 ttgcttctgc tgcccgcatg cgtcctaacc agaaatttga gtggcatcat tttggcaatg
      901 gtgatcaacg agagtttctt cagcaacagg cagatacatc tttcccatca aatgccaaag
      961 cgtttttacc aggattttcg gatggtaatg ctctcatgga ttactatcga aataacccca
     1021 ttgatgtttt tgtgaattgt agcagtacag aaggtacatc tgttgctatt atggaggcaa
     1081 tgagttgcgg cattcccatc cttgctacaa gtgtaggtgg aaacgtggag attgtttcag
     1141 aaaaaaatgg tctattgatc cccgctacag tgacaagctt tgaaattgcc gccggtttac
     1201 tggcatttgt agatgaccct gaaccttcgg caaaacgcca aggcagcaga caaaaatggt
     1261 ctgtcgaata taatgcagat gttaatttcc ccgaatttgc ccatgatttg aagttgattc
     1321 gtttaggcaa agcggacaaa taggagattt tttatgtgtg gaatttttgg cgtttggaat
     1381 gtgcaaaaac accctcttga cttgcaagcc atgcttgatt ctgttcatgc catccgtcat
     1441 cgcggaccgg atgatgaagg ctttttgctc attgataccg aagcccatga tgcagtccct
     1501 tgctctggtg tagatagtgc tactcaattg gggttgaggc ggattgaaga ctgttatcaa
     1561 cctcgttttg acttgttctt tggttaccgt cgcctatcga tcattgatgt ttctccagcg
     1621 ggacatcaac ctatgagcaa tatagatggt tcgctttgga tcgtttttaa cggcgagatc
     1681 tataactata ttgagctgcg cagcgaactt cagtcgcttg gctatgtttt tcgcacacaa
     1741 acggacactg aagtgattct taatgcctat catgcttgg
//
LOCUS       DAOQJE010000813         1775 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_58174_length_1775_cov_6.728488, whole genome shotgun sequence.
ACCESSION   DAOQJE010000813 DAOQJE010000000
VERSION     DAOQJE010000813.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1775)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1775)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1775
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_58174_length_1775_cov_6.728488"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..409)
                     /locus_tag="PLT08_16815"
     CDS             complement(<1..409)
                     /locus_tag="PLT08_16815"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: protein motif:HMM:NF014021.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HNE06190.1"
                     /translation="MDTDKLIQTMVDEVKTVSGVKAIVLGGSRARGTYTPSSDIDLGI
                     YYDPNQPMDLTALGKIATQLDDEHRTDVITGFGGWGPWINGGGWLRVQSLPVDFLYKD
                     LGRVKSYIDACLNGKVEIFYQGGHPHGFVTSIYL"
     gene            complement(437..841)
                     /locus_tag="PLT08_16820"
     CDS             complement(437..841)
                     /locus_tag="PLT08_16820"
                     /inference="COORDINATES: protein motif:HMM:NF022479.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2721 domain-containing protein"
                     /protein_id="HNE06191.1"
                     /translation="MEELTLTTPALLFPTVSLLMLAYTNRFLTLATIIRNLNDRYHDS
                     QDKNLLAQIDNLRYRVYLIRNMQIFGVLSLLMCVVSMFALFFNWTDGGVWSFGIALVL
                     MIISLGISLRELQISVGALDLLLTNLEEHETK"
     gene            complement(874..1389)
                     /locus_tag="PLT08_16825"
     CDS             complement(874..1389)
                     /locus_tag="PLT08_16825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE06192.1"
                     /translation="MRALLPDEKLSTVFIYTPTMLVRGDLILRENIRASIWLRTQGVP
                     NYIHLYAAQVIQLAGSPPKNYTSQETFIPTAEVIGFHLAPPAQDPLDYDATEANRKME
                     SVHVLAGSFEMKAKLRISTSADFASGLDVMNTSWLSLYEAEITNPYLPQLKLTVPMLL
                     ARPSKLTISLL"
     gene            1465..>1775
                     /locus_tag="PLT08_16830"
     CDS             1465..>1775
                     /locus_tag="PLT08_16830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE06193.1"
                     /translation="MTQDKLWNISPRISPEADSALAAFPPLLRQVLFNRGFADDPSAR
                     AYLRGEPNSNTNPFQITDMQVAIERIRYALANNEPIAIYGDYDVDGVTATALLVETLK
                     KL"
BASE COUNT          524 a          473 c          350 g          428 t
ORIGIN      
        1 ccaaataaat tgaggtcaca aatccatgcg gatgcccgcc ttggtaaaaa atttcaactt
       61 tgccatttaa acaagcatca atataggatt taactctccc caaatcttta tacaaaaagt
      121 ctaccggcaa cgattgaact ctcaaccacc cgccaccatt gatccacgga ccccaaccgc
      181 cgaagccggt aatgacgtcc gttcggtgtt cgtcatcaag ctgagtcgct atcttaccca
      241 aagcggtcaa atccatgggc tgatttgggt catagtaaat tcccaagtca atgtcagaag
      301 aaggggtata cgtccctctg gcacgcgagc cgcctaacac aatggctttt acgcctgaaa
      361 ccgttttgac ttcatcgacc atggtttgaa taagtttatc cgtatccaca accacctaca
      421 ctttcagaga cgctctttat ttcgtctcgt gttcttccaa atttgtcaac agcaaatcca
      481 aagcgcctac tgaaatttgt aattcacgca aagaaattcc caacgagata atcatcaaca
      541 ccaaagcgat gccaaaactc cacacaccac catccgtcca attaaagaac aaagcgaaca
      601 tgctgacaac acacatcaac aggctcaaaa caccgaagat ctgcatattt cgaatgagat
      661 acacgcgata acgaaggtta tcgatctgcg ccagaaggtt cttatcctgg ctatcatgat
      721 agcggtcatt caaattacgg atgatcgtgg caagggtcaa aaaacgattc gtgtacgcca
      781 acatcagtag cgacaccgta ggaaacaaaa gtgctggggt ggtaagggtc agttcttcca
      841 tagtaaatac tcaagcggca tatgccgcac attctataac aaactaatcg tcagtttact
      901 ggggcgtgcc aacaacatag gcactgtcaa cttcaattgc ggcaaatacg gatttgtgat
      961 ctctgcttca tacagagaaa gccaggatgt attcatcaca tccaaaccgg aggcaaaatc
     1021 ggcggaggta gagatgcgca acttggcttt catttcaaaa gagccagcca acacatgcac
     1081 agactccatt ttacgattcg cttccgtcgc atcataatcc agcgggtctt gagcaggagg
     1141 cgcaagatga aaaccgatca cctctgcagt aggaataaaa gtttcctggc tggtgtaatt
     1201 cttgggcggg gaacctgcca attgaatcac ctgcgccgca tacaagtgaa tgtaatttgg
     1261 cacaccctga gtacgcaacc aaatacttgc gcggatattt tctcgcaaga tcaaatctcc
     1321 acgcaccaac atggtaggcg tataaataaa cacagtcgat aatttttcat caggcaataa
     1381 agctctcatg cgtcgctcct caacagatta tactcgcgcc cttcctcttg actttggtct
     1441 cgtttatact ccgcacactt taacatgaca caagacaaac tttggaacat ctcgcctcgc
     1501 atctcccccg aagctgattc agctttggca gcctttcctc cgctgttacg tcaggtgctt
     1561 ttcaatcgcg gctttgccga tgaccccagc gcacgtgcct atttgcgcgg cgaacccaac
     1621 agcaatacca atccttttca aatcaccgat atgcaagtcg caatagagcg catccgctat
     1681 gcgcttgcaa ataacgaacc cattgccatt tatggtgact acgatgttga cggagtcact
     1741 gctaccgcat tattggtgga aacgctcaaa aaact
//
LOCUS       DAOQJE010000814         1772 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_58374_length_1772_cov_3.893419, whole genome shotgun sequence.
ACCESSION   DAOQJE010000814 DAOQJE010000000
VERSION     DAOQJE010000814.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1772)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1772)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1772
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_58374_length_1772_cov_3.893419"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..339)
                     /locus_tag="PLT08_16835"
     CDS             complement(<1..339)
                     /locus_tag="PLT08_16835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE06194.1"
                     /translation="MGIDIRWENESGNQLEEISDPKNYFGFALALSPLEKTVCLRFID
                     PYGDTVFNQQQIPVFISELQALQQLVTSEQLDGLREKLKMEHQEYNSTKTETRTAIEI
                     SEHITKILELA"
     gene            complement(498..959)
                     /locus_tag="PLT08_16840"
     CDS             complement(498..959)
                     /locus_tag="PLT08_16840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE06195.1"
                     /translation="MTDTQPSSDINSGSDDALTPRIRLSLILTFIGLFIFAVGAKPDL
                     FGWDRSPVVGFVQIIVFLIGLAWICVGGYLGLHALWWGLERTIIGDIGSRLVSTGFVF
                     SVFAGLADIIGMGSHSFPQIPYFGPWQATGVLIGQAIIALGFLMMIPFKHK"
     gene            complement(966..>1772)
                     /locus_tag="PLT08_16845"
     CDS             complement(966..>1772)
                     /locus_tag="PLT08_16845"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: protein motif:HMM:NF013413.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070573 - metallodipeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane dipeptidase"
                     /protein_id="HNE06196.1"
                     /translation="DVYHRMQDDMPDKFRILASKRDLDLHLTQWSESTPGASKPLGMV
                     VLMEGGEAIRELSELEMWHNRGVRLIGPAWVGTRYCGGWKEPGPLTDDGRKLLAAMAD
                     FNFTLDLSHMDERAAIEALDIYEGPIVGTHANCAALMPNSNTNRHFSDRIIEGIIERD
                     GVVGVVPFNTYLKVGWSREKGHRREEVPLDVLANHIDHICQIAGDSLHAGIGSDFDGG
                     FGLQSVPPEIETVADLQKLVSLLQARGYSETDTTNILGANWIARLKRDLP"
BASE COUNT          456 a          445 c          449 g          422 t
ORIGIN      
        1 agccaactct aaaatcttgg tgatatgttc tgaaatttca attgcagtac gtgtttctgt
       61 tttagtggag ttatactctt gatgttccat tttgagtttt tcgcgtaaac catcgagttg
      121 ctctgatgta acaagttgtt gcaaggcttg gagttcactt atgaaaacag gaatctgttg
      181 ctgattgaat acagtgtcgc cgtaggggtc aataaaacgt aagcaaacgg ttttctctaa
      241 tggtgataaa gccaacgcaa acccaaaata atttttcggg tcagaaattt cttctaactg
      301 attgccagat tcgttttccc agcgaatgtc aattcccatg attttttcct gcaaggtttt
      361 gcccaactac tgtttataca gcgcgctgtc taaacaccca aaatggggta cataacagcg
      421 atttcgctgg ttaacgccct atatactgca aattgtacac ccttgttttc gacgcgatca
      481 ataagatagg ttgagaacta cttatgtttg aaaggaatca tcatcaaaaa tcccagcgca
      541 atgatagcct gaccgatcaa gacgccggtc gcttgccagg gaccgaagta tggaatttgc
      601 gggaaggaat gagaccccat gccgatgatg tcggctagac ctgcaaagac cgagaacaca
      661 aaaccagtag agaccaagcg actaccaata tcaccgatga tggtacgttc cagtccccac
      721 cacagggcgt gcaagccgag gtagccgccc acacaaatcc atgccagacc gatcaggaag
      781 acgatgatct gcacaaagcc aaccaccggg ctacggtccc agccaaagag gtctggtttg
      841 gcgcctacag caaagatgaa gagtccaata aaggtgagga taaggcttaa gcgaatgcgc
      901 ggcgtaagcg catcatcact gccagaattg atgtcagaag aaggttgggt gtcagtcata
      961 tgggtttaag gtaaatctcg tttgaggcgg gcgatccaat tggcaccgag aatattggtg
     1021 gtgtctgttt cagaatagcc gcgcgcttgc aataaggata ccagtttttg taaatctgcg
     1081 acggtctcaa tttctggcgg aaccgattgc aacccaaagc cgccgtcaaa atcggagcca
     1141 atgccagcat gcaacgagtc accggcgatc tggcatatgt ggtcaatgtg attagcgagc
     1201 acgtccaacg gcacttcttc gcggcggtgt cccttctcgc gtgaccaacc caccttgaga
     1261 tacgtattga acggcacgac tcccaccacc ccatcgcgct cgatgatgcc ctcgatgatg
     1321 cgatcactga agtgccggtt cgtgttggag ttgggcatga gtgccgcaca gttggcatgg
     1381 gtgcccacga tgggaccttc ataaatatcc aaagcttcga tggcggctcg ttcgtccatg
     1441 tggctgaggt cgagggtgaa gttaaagtct gccatggcag ctaacaactt gcgcccgtca
     1501 tcggtcaacg gaccgggttc cttccagccg ccgcaatagc gtgtgcccac ccaggcaggt
     1561 ccgatcagac gcacgccgcg gttgtgccac atctccaatt cagagagttc gcggatcgct
     1621 tcgccgcctt ccatcagcac caccatgcca agcggtttgg aagcccccgg cgtggattcg
     1681 ctccactggg tcagatgcag gtcaaggtct cgtttggagg cgagaatgcg gaatttatcc
     1741 ggcatatcat cctgcatgcg atgatagaca tc
//
LOCUS       DAOQJE010000815         1770 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_58521_length_1770_cov_3.600583, whole genome shotgun sequence.
ACCESSION   DAOQJE010000815 DAOQJE010000000
VERSION     DAOQJE010000815.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1770)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1770)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1770
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_58521_length_1770_cov_3.600583"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            98..>1770
                     /locus_tag="PLT08_16850"
     CDS             98..>1770
                     /locus_tag="PLT08_16850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE06197.1"
                     /translation="MVKINVDLPQILVSTPNYKWEWNVATDTFWLHDKNQRIISRAQH
                     RPLLLTSPTPLQFTNATHKIQGDQIHITYQGPDPASSLTVVWSFHTDYISLEPFLLTL
                     PEDIDLVSIVYFPTIEGDSYKPSMYSHYAVVPGLTMSTNISPIVDLHSRLNTTAVLGS
                     GAMRGPGLTQQWGLPAHYFCTFNTSDRWNAIGAKAQQSEAACWGLATLPQGDFRLEMR
                     EVGISPVLNLRTDLWKHKPDTFGFHFLITFGENYHEAIRKYYQVLLREKFISPKKHSP
                     KKQEVLISPQYNTWGVESALALPPEQLTEELVRDIFIKFKASGMKAKTFVIDDKWEGT
                     YGELKHDPERFPNFESLLKDIRAQGYNIGLWAAFLRCQNPAALGLDESHLLQTHEGKP
                     LWLDHQTSRYGIFDMTQPKVQEVLRQRAKDFIRRYQPDLIKFDFGYELPSLDVSAPQD
                     MNFAGERLLQKGLEVIVGAMKEENPDLVIMYYGLSPLLIDYYDLHSPDDLVYCVGDYD
                     LESNRRILFSSLCGELGMPTYSSSGYDWDSAIDIWFDTVASGSLGSLHS"
BASE COUNT          450 a          515 c          369 g          436 t
ORIGIN      
        1 atttgatata atgtgaccgg tcacgtgaac ggtaacatta gtctacagaa aaataaaccc
       61 tttgtcaagt aggcaatttc caaattgagg tgttttgatg gtgaaaatta acgtggatct
      121 tccccaaatt ctggtttcca ccccaaacta caagtgggaa tggaatgtcg ctacagacac
      181 attttggcta cacgacaaaa accaacgcat catttcccgt gctcaacata gacccctact
      241 cctcacctcc cctacgccgt tgcaatttac aaatgccacg cacaaaatcc aaggcgatca
      301 gattcatatc acctatcagg gaccagaccc cgcctcttcc ttaactgtag tctggtcctt
      361 ccacacagac tatatctccc tcgaaccttt cctcctcacc ctccctgaag atatagacct
      421 cgttagcatt gtgtacttcc ccaccatcga aggtgactct tacaagcctt ccatgtacag
      481 ccattatgcc gttgtgcccg gcttgaccat gagcaccaac atcagcccca tcgtggattt
      541 gcattcccgc ctcaacacca ccgctgtgct cggctctggc gccatgcgcg gaccagggct
      601 gactcaacaa tggggtttgc ctgcgcatta cttctgcacc ttcaacacat ccgaccgttg
      661 gaatgccatc ggcgcgaaag cccaacaaag cgaagccgcc tgctgggggc tcgccacctt
      721 accgcaaggt gacttccgct tggagatgcg cgaggtcggc atcagcccgg tgctcaacct
      781 ccgcacagac ttgtggaaac acaaacccga tacatttggc tttcacttcc tcatcacttt
      841 tggcgagaat tatcacgaag cgatccgcaa gtattaccaa gtcttactgc gcgagaagtt
      901 catttcacct aaaaaacatt cgcccaaaaa acaagaagtt ttgatttccc ctcaatacaa
      961 cacttggggc gtagagtctg ctttggcact tccgcctgaa caactcaccg aagaactcgt
     1021 gcgcgatatt ttcataaaat tcaaagcctc aggcatgaaa gccaagacct tcgtcatcga
     1081 cgacaagtgg gaaggcacgt atggcgaact caagcacgac cctgaacgct ttcccaattt
     1141 tgaaagcctg ttaaaagaca tccgcgcgca ggggtacaac atcggcttat gggcggcatt
     1201 cctacgctgc caaaatcccg ccgcgcttgg cttggatgaa tcacacctgt tgcaaacaca
     1261 tgaaggaaaa ccgctctggc ttgaccacca aacgtctcgt tatggcatct tcgatatgac
     1321 ccaacccaaa gtgcaagaag tgttacgtca acgtgccaaa gacttcattc gccgttatca
     1381 acctgacctg atcaaattcg acttcggcta cgaactgccg tcgctcgatg tttccgctcc
     1441 gcaagatatg aacttcgctg gagaacgcct cttgcaaaaa ggcttggagg tcattgtggg
     1501 tgcaatgaaa gaagagaatc cagaccttgt catcatgtac tacggtcttt ctcctctact
     1561 cattgattac tacgacctgc acagccctga tgacctcgtc tactgtgttg gtgattatga
     1621 ccttgagagc aaccgtcgca tccttttcag cagtttatgc ggtgaactgg gtatgcccac
     1681 ctatagttcc tctggctacg attgggactc tgccattgat atttggtttg ataccgttgc
     1741 ttcgggcagc ctcggctcgc tccactcttt
//
LOCUS       DAOQJE010000816         1769 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_58598_length_1769_cov_3.885064, whole genome shotgun sequence.
ACCESSION   DAOQJE010000816 DAOQJE010000000
VERSION     DAOQJE010000816.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1769)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1769)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1769
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_58598_length_1769_cov_3.885064"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..545
                     /locus_tag="PLT08_16855"
     CDS             <1..545
                     /locus_tag="PLT08_16855"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNE06198.1"
                     /translation="LMARGLKKSAPEDFKEKLAELESLASDTLKEMRLMLDQLREASG
                     EEKVDFAESVRAECEAFSNRSGPEGGALLSVTLEMPRVVIIQKNIANEATWVLREALQ
                     NVVKHSGGRSVTVRVSVDAFLHVSVTDDGAGFDVHAMPTGHYGVRGMRERVLALGGEL
                     TIDSEIGRGTTLNYKIPLPR"
     gene            548..1186
                     /locus_tag="PLT08_16860"
     CDS             548..1186
                     /locus_tag="PLT08_16860"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HNE06199.1"
                     /translation="MLKIIIADDHQVVRRGLTLTINAEKDMRVVGEAQNGAGVLALIK
                     KHKPDVVLMDLQMPEMTGVDALKQIRPEYPDLPILILTSFTDDAHVFAALRAGASGFL
                     LKEMNGDDLVAAIRGAAQGKPQLHPDIAKRLMARAPMPDDPFDSLTERERGILKLLAK
                     GKSNKEIAGELSLTEMTVKGYVSDMFVKLGVNDRTQAALMAVRYGLVLPEEL"
     gene            1330..>1769
                     /locus_tag="PLT08_16865"
     CDS             1330..>1769
                     /locus_tag="PLT08_16865"
                     /inference="COORDINATES: protein motif:HMM:NF012296.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HNE06200.1"
                     /translation="METNLVLEQPVYKTSTFEFLWNVFRNPLHALTDLAHEQGDIARV
                     KLRKRDLFLLSHPAFIEQVLVRQADNFVKGPSLQRARIILGEGLLTSEGDEHLTQRRT
                     LQPAFHRHKMEEYIPLMNENTMAHIANWQDGERADMSEGMMRLTL"
BASE COUNT          430 a          361 c          545 g          433 t
ORIGIN      
        1 atttgatggc acgcggattg aaaaagtctg cgccggagga ttttaaagaa aagctggcag
       61 agttggagag tctggcgagt gacacgctca aagaaatgcg gctgatgctc gatcaactgc
      121 gcgaagcatc cggcgaggaa aaggttgact tcgctgaatc cgttcgagcg gagtgtgagg
      181 cgttttccaa ccgaagcgga cccgaaggcg gggcgttatt gtctgtaacg ctggagatgc
      241 cgcgggttgt aattatccaa aagaatattg cgaatgaagc gacgtgggtg ctgcgcgagg
      301 cattgcaaaa tgtggtgaag cacagcggtg ggcggagtgt gacagtgcgc gtgagtgttg
      361 acgcgttttt acatgtgagt gtgacagacg atggcgcagg gtttgatgtg catgccatgc
      421 ctacgggaca ttatggtgtg cgcgggatgc gtgagcgtgt gttggcgttg ggtggcgagt
      481 tgaccattga ttctgaaatt gggcgcggta cgacgttgaa ttacaagatt cctttgccga
      541 ggtaaatatg ctgaaaataa tcattgctga tgatcatcag gttgtgcgcc gtgggttgac
      601 cttgaccatt aatgctgaga aagatatgcg tgtggtgggg gaagcgcaga acggcgcggg
      661 tgtgttggcg ttgatcaaaa aacataagcc cgatgtggtc ttgatggatt tgcaaatgcc
      721 cgagatgacc ggtgtggatg cgttgaagca gatccgcccg gaataccctg atttgccgat
      781 tctgattctg acttcgttca ctgatgatgc gcatgtcttt gcggcgttgc gtgcgggtgc
      841 gagcggattt ttgctgaagg aaatgaatgg cgatgatttg gtggcagcga tccgtggagc
      901 ggcgcaaggc aaaccgcaat tgcaccctga tattgcgaag cgcctgatgg cgcgtgcgcc
      961 catgcctgat gacccgttcg attcgttgac cgaacgcgag cgcggcattt tgaaattgct
     1021 ggcaaagggt aagagcaata aagaaattgc tggcgagtta tcgctgaccg agatgacggt
     1081 gaagggctat gtcagtgaca tgttcgtgaa gttgggggtg aacgatagaa ctcaggcggc
     1141 gctgatggcg gtccgctatg ggttggtttt gccggaagaa ttgtagaact caaagtgaca
     1201 gtcaccttta aggtgactgt cacttttttt atatacccac caaatagggg gagaggttcc
     1261 ccaccttcgc gggcggactt aatcggttca aaaaagtatc ctttggttat cacttgaagg
     1321 agatagccga tggaaacaaa tttagttctt gaacaacccg tttacaaaac atccaccttt
     1381 gaatttttat ggaatgtctt tcgcaacccg ttgcatgcgt tgaccgatct cgcgcatgaa
     1441 cagggtgata ttgcacgagt caaattgcgc aagcgtgacc tctttctact gagccacccg
     1501 gcatttattg aacaggtctt ggtgcggcag gcagataact ttgtgaaagg tcccagcttg
     1561 cagagggcac gcatcatttt aggcgaaggg ttactcacca gcgaagggga tgaacacctt
     1621 actcaacgtc gcaccttgca acccgcgttt catcgtcaca agatggaaga atacattccc
     1681 ttgatgaacg agaatacgat ggcacatatt gcgaactggc aagatggcga gcgggcagat
     1741 atgtctgaag gtatgatgcg tctgacgct
//
LOCUS       DAOQJE010000817         1767 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_58716_length_1767_cov_6.530958, whole genome shotgun sequence.
ACCESSION   DAOQJE010000817 DAOQJE010000000
VERSION     DAOQJE010000817.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1767)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1767)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1767
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_58716_length_1767_cov_6.530958"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(110..796)
                     /locus_tag="PLT08_16870"
     CDS             complement(110..796)
                     /locus_tag="PLT08_16870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE06201.1"
                     /translation="MHLTGGSLRHFRAFSTPEQNPALGVLSQPAHPQVTQTVGWQGQK
                     QTQGNINIMRTNKTKLFMGVLVGLIAVSIIGILYYRVTAIIEDVFAVPVPPSAILQVQ
                     NMEQTAKLGAFNWDGHWADVYAIITPRDVLVVNSPIAATLKLSIDIPPSALSISVTPA
                     SKIKEFPEDSATIRVWELNPKNYNKRNDLLLKQEQTINLDLEHGLYVIVVNAGWQQGS
                     VTYGFLIDVQ"
     gene            complement(803..1465)
                     /locus_tag="PLT08_16875"
     CDS             complement(803..1465)
                     /locus_tag="PLT08_16875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE06202.1"
                     /translation="MVKFIGIYLGRFFGIILGGFLGHEVADTLKQSNLIGVIIGALAF
                     YFAGRWIGSKISVLIGSQLDKVLSIPESQATVNIVETKSTSRFASIGFILYGVILPFL
                     LVVIGLLMDYFDIPIGYLPELLPVSRIVVILFSIFSICAPWFLKSRWISKYQTLTSSP
                     ESIVYWLGLVFSIVPAIYGFVLFLAMGASIIELCLYAAASSIAAIIWNMNNKVALKQR
                     AG"
BASE COUNT          553 a          376 c          379 g          459 t
ORIGIN      
        1 cgcagaatcc ccagcgtcag gtgcacgctt tgttggctgg cttttttgaa cgcgagactt
       61 tcaaccgaag aatgataatg atgaggcata tatttgatag ttatcttgat tattgtacat
      121 caataagaaa tccatacgtc acgctgcctt gctgccaccc agcattgacg actattacat
      181 ataacccatg ttcgaggtca agattaatag tttgttcttg tttcagcaac aggtcattcc
      241 gcttgttata gtttttgggg ttcagttccc acacacgtat agtagcagag tcttctggaa
      301 attcctttat ttttgacgca ggggtgacac tgatacttaa agcgctgggt ggtatatcta
      361 tagataattt tagtgtggcg gcgattgggg agttaacaac taagacatct cgcggtgtaa
      421 ttatggcata aacatccgcc caatgtccgt cccagttaaa tgctcctaac tttgctgttt
      481 gctccatgtt ttgtacctgc aaaatagctg aaggtggaac aggcacggca aatacatctt
      541 caattattgc tgtaacacga taatataaaa tacctattat tgacacggca attaatccaa
      601 ccaagacgcc cataaaaagt ttagttttat ttgttctcat tatattaata ttgccctgtg
      661 tttgtttttg gccctgccag ccaacggttt gcgttacttg cgggtgggcg ggctgagata
      721 aaactccgag agcaggattc tgttcgggcg tagaaaaagc ccgaaaatgc cgcagactcc
      781 cacccgtcaa gtgcacgctt tgttagcccg ctctttgctt tagagcgact ttattattca
      841 tgttccagat tatagccgcg atggaagatg ccgccgcata gagacataat tcgataattg
      901 aagcgcccat tgcaagaaac aaaacaaaac cataaatagc agggacaata gaaaagacga
      961 gacccaacca atagacaatg gattctggtg aagaagtcaa agtttgatat ttacttatcc
     1021 accgactttt caaaaaccag ggagcgcaaa tcgagaagat ggagaacaat ataactacaa
     1081 tacgtgagac aggcagtaat tcagggagat agccaatcgg aatatcaaaa tagtccatca
     1141 gcaaaccgat taccacgagc aaaaacggaa gaataacgcc gtataaaata aagcctatcg
     1201 aagcgaatct gcttgttgat tttgtttcta caatattcac tgttgcttga gactctggaa
     1261 tggataagac tttatcaagt tgtgaaccga ttagaacact gattttagaa cctatccagc
     1321 gaccagcaaa ataaaaagcc aacgccccga taataacgcc gatgagattt gactgtttga
     1381 gggtgtcagc gacttcatgc cctaaaaacc caccgagtat gattccaaaa aagcgaccaa
     1441 gataaatgcc aataaacttc accaaaaagt ctttgttatt ggctttcttg tgtgaaaacc
     1501 acgaaacaaa acctacaagc gcgcctttcc aaactagcag gaaagtaatg attagtaatc
     1561 caatccccga taacttcacc gagaagagag caagcccaac cgcgctaatc agaagaaaaa
     1621 caaggttggt gagactcgaa atgaggatga aatacaaaaa tggcgagatc gtttttgggg
     1681 aactttgcat aaaacatcct tcaatcttgc acgggcgggc taacggtttg cgttacctgc
     1741 gctggggtgg ggacggcgaa gccgtcc
//
LOCUS       DAOQJE010000818         1766 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_58799_length_1766_cov_5.144944, whole genome shotgun sequence.
ACCESSION   DAOQJE010000818 DAOQJE010000000
VERSION     DAOQJE010000818.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1766)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1766)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1766
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_58799_length_1766_cov_5.144944"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            239..1555
                     /locus_tag="PLT08_16880"
     CDS             239..1555
                     /locus_tag="PLT08_16880"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNE06203.1"
                     /translation="MINRFRNWASTPIIEDENKSLSANLLNLIIWILVISTSLYGIIA
                     PVEPEFLNQRLFFIIPYILINLVAKQLLNNGYIDLVGHFIVICLWGVITSALFVGADN
                     KNPAFMGYIVVVTCAGLVLNRKAAILWALISIITSALILQFGILGFLPRSANETPAFA
                     FWSAQALYIAVCSMLLTQTLQKIEEARNRAQKELLERKRVEAERERIIGELEMKNAEL
                     ERFTYTVSHDLKSPLITIGGFIGLLEKDTLNGDVNRLKNDLEKIREAKDKMARLLDEL
                     LELSRIGRVKNPSGEFSLKQIVDEAVTLVQGRITSGRVQVIIHEPLPMLYCDGPRLVE
                     VMQNLLDNAAKFMGDQPTPRIDISARKDADEIIVYVKDNGMGINPIFHTKIFNLFDKL
                     DPKSEGTGAGLALVKRIIEVHEGRIWVESDGAGTGSCFCFTLPAKT"
BASE COUNT          512 a          347 c          404 g          503 t
ORIGIN      
        1 ctgctctgcc tgaagtaaaa cttgaagatg tagagaaggc gcttgaaggt catttgcctg
       61 cccgccacaa acatctcttg ccaaagaact tcgaggctct gaaacgcggc tttgaacatg
      121 cgcaaaaaga atgggataaa gtcccagctt aaataaactg ataataaaag tccatctcta
      181 atttctgaga tgggctttta tttatggttt aatcaggttt attctgctgg agactcttat
      241 gataaatcga tttcgcaatt gggcaagtac acctatcatc gaagatgaaa ataaaagcct
      301 ttctgcgaat ctgctcaacc tgatcatttg gatattggtt atttcaacga gtttatacgg
      361 tattatcgcc ccagttgaac cagaatttct aaatcagcgc cttttcttta tcatccccta
      421 tatccttata aatttagttg caaaacaact cctcaacaac gggtatatcg acttggttgg
      481 gcattttatt gtgatctgtt tatggggagt cattaccagt gcattattcg taggggcaga
      541 taataaaaac ccagccttta tgggctatat tgtggtggtg acttgtgccg ggttggtctt
      601 aaaccgaaaa gccgccatct tatgggcgtt gatatccatt attaccagtg ccttgatctt
      661 acaattcggt attcttgggt ttttaccacg ctcggcaaat gaaacacctg cctttgcttt
      721 ttggtctgct caagccttgt atattgcagt ctgctccatg ttattaacgc aaactcttca
      781 gaaaattgaa gaagctcgca atcgagcgca aaaagaattg cttgaacgca aacgagttga
      841 agcagaacgc gaaagaatca ttggtgagct tgagatgaaa aatgcggagt tagagcgttt
      901 tacatacacc gtctcacatg acctaaaaag cccgctaatc actatcggcg gctttatcgg
      961 gctactcgaa aaagacacac ttaatggaga tgtaaatcgg ctaaagaatg atttagaaaa
     1021 gatccgcgaa gcaaaagata aaatggcgcg tctgttagac gagcttttgg agctctcacg
     1081 tattgggcga gtgaaaaacc catctggtga attttcattg aaacagatcg tcgatgaggc
     1141 ggttacattg gttcaaggca gaatcacatc tgggagagtg caagtgataa ttcatgaacc
     1201 tttaccaatg ttatattgtg atggtccgcg gctggtagag gtaatgcaaa atctattgga
     1261 caatgccgca aaatttatgg gagaccagcc cacaccgcga attgacatca gcgcaaggaa
     1321 ggatgccgat gaaattatcg tatacgttaa agacaatggc atgggtatca atcccatttt
     1381 tcataccaag atctttaatt tattcgacaa actagatccc aaaagtgaag ggactggtgc
     1441 cgggctcgcg ctggtcaagc gcatcattga agtccatgaa ggtcggatct gggtcgagtc
     1501 agatggtgcc ggtacaggta gttgtttctg ctttacatta cccgctaaaa cgtaacctca
     1561 aatggcataa ggtaataggt ttattaaaaa tccgcattcc agactggaat gcggattttt
     1621 tgtgtacaat gattttgtcg aaccgagagc taacttaata gtttatggag gaaatatgca
     1681 cgaaattaca ttgatcatca atattgttgt ggcgttggtg gtggctttta ttggcggagt
     1741 catcgcgcgg cggcttgggc tgccta
//
LOCUS       DAOQJE010000819         1765 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_58875_length_1765_cov_4.184211, whole genome shotgun sequence.
ACCESSION   DAOQJE010000819 DAOQJE010000000
VERSION     DAOQJE010000819.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1765)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1765)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1765
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_58875_length_1765_cov_4.184211"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(5..391)
                     /locus_tag="PLT08_16885"
     CDS             complement(5..391)
                     /locus_tag="PLT08_16885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE06204.1"
                     /translation="MIYHNASALHGIIASRQGEQGDEVAARGAFVRAIGQADEILSKT
                     AEYYSALDAKGLAICGLVLCRGAAPLRPYAQDIDNAIETFKKARRIAPHAGVVKSVLR
                     LFDELAKCDEKDVLKGTREVLEGKGT"
     gene            complement(491..1165)
                     /locus_tag="PLT08_16890"
     CDS             complement(491..1165)
                     /locus_tag="PLT08_16890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE06205.1"
                     /translation="MLIYFIFPLIVVLIGLLIEYWVIQPLRKNGIPISFQTTVSSIIQ
                     RMTSFIKPDKINEESSPSEKKTSLNRILKSKLGIGITLIIFLLLLTASYFLIKANQKP
                     VYTYPTPIRYSIAIGDNLNKVIDIDQTHEYVFTSSSNKIVAITIRRIRTTDEAKRNLR
                     VNLYDENYVYLFEESILADTTDPAEKTIEFAPEIGVNYYLEIYLGSSPSNDYFGANYI
                     ISLEYK"
     gene            complement(1236..>1765)
                     /locus_tag="PLT08_16895"
     CDS             complement(1236..>1765)
                     /locus_tag="PLT08_16895"
                     /inference="COORDINATES: protein motif:HMM:NF025056.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003953 - NAD+ nucleosidase activity
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="toll/interleukin-1 receptor domain-containing
                     protein"
                     /protein_id="HNE06206.1"
                     /translation="LEGEHQHLKSERSVNRIFLSYRRADSADVVGRIYDRLIQVYKKE
                     FVFKDVDSIPLGLDFREYLHRMVSISTVFIAVIGKNWESVQDEQGNRRLNNPADYVRI
                     EIASALSRNIPVIPVLVQNSSMPLVDDLPDDLKNLCYRNGISVRPDPDFHNDMDRLIS
                     GLSKLEDINNKNFFK"
BASE COUNT          484 a          376 c          333 g          572 t
ORIGIN      
        1 atggttatgt tccctttcct tctaaaacct ctcgtgtccc tttcaaaaca tccttctcgt
       61 cgcattttgc caattcatcg aacaaccgca gcacggactt gaccaccccc gcgtgcggcg
      121 cgattcttcg cgccttcttg aatgtttcga tggcattgtc tatatcttgg gcgtacgggc
      181 gcagcggcgc tgcgccccga cagagcacga gcccgcaaat cgccaacccc ttcgcatcca
      241 gcgcggaata atactccgcc gttttggaca ggatctcatc cgcctgcccg atcgcgcgga
      301 caaaggctcc ccgcgccgcg acctcgtccc cttgctcccc ttgacgggag gcgatgattc
      361 cgtgcagagc actggcattg tggtatatca tgttgcaatg cggctttctc gttgatgcat
      421 aaatactcac ttaacatatt caacctatgt ttacaacaga gcatgtgcat attatgtagt
      481 gcctttactg ctatttatat tctaggctga taatgtagtt cgcgccaaaa taatcattgc
      541 tgggagagga gcctaaataa atttcaagat aataattgac ccctatttct ggtgcaaact
      601 caattgtttt ttcagctggg tccgtggtat ctgcaagtat tgattcttca aatagataga
      661 catagttttc gtcgtataga ttgactctca ggttcctttt cgcttcatca gtggttctaa
      721 ttcgtctaat tgtgatagcc acaattttat tggagctcga tgtaaaaaca tattcatgag
      781 tttgatcgat atctataact ttattcaagt tatcgccaat tgcaattgaa tatcttatgg
      841 gagtagggta ggtgtagact ggcttttgat ttgctttaat taaaaaatag cttgcagtta
      901 ataataacaa aaagataatt aaagtgatac caatgcccaa cttagatttt agtatcctat
      961 tcaaggaagt ctttttttcg gaaggcgaac tttcttcgtt tattttgtca ggctttataa
     1021 atgacgtcat tctctgaatt atgcttgaaa ctgtcgtttg gaagctaatg ggaattccgt
     1081 ttttgcgtaa tggttgtata acccaatatt cgataagcaa tccaatcaac acgacaatta
     1141 aaggaaaaat aaaataaatt aacatttatg gacacctatg ttgaatatct gatgaagcaa
     1201 tgatttttca ttacattttc tttctactgc aatcactact taaaaaagtt tttgttattg
     1261 atgtcttcta gcttcgataa tccactaata agacgatcca tatcgttgtg aaaatctggg
     1321 tcaggtcgta cggaaatacc attcctgtaa cataaattct ttaagtcgtc tggtaaatcg
     1381 tcaactaatg gcatagaaga gttttgtaca agaactggaa taactgggat gttgcgagaa
     1441 agcgctgagg caatttcaat tcttacataa tcggccggat tattcagtcg gcgatttcct
     1501 tgttcatctt gcacactttc ccaatttttt ccaataacag cgatgaatac cgttgaaatt
     1561 gaaaccattc tatgtaaata ttctcgaaaa tccaatccta atggaattga atctacatcc
     1621 ttgaatacga attctttttt gtatacttgg attagtctgt catagattcg gcctaccaca
     1681 tccgcactgt ctgctctgcg atatgaaaga aaaatacgat taacggatct ctcacttttc
     1741 aggtgctgat gttctccttc aagtt
//
LOCUS       DAOQJE010000820         1764 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_58945_length_1764_cov_4.808660, whole genome shotgun sequence.
ACCESSION   DAOQJE010000820 DAOQJE010000000
VERSION     DAOQJE010000820.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1764)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1764)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1764
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_58945_length_1764_cov_4.808660"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..398)
                     /locus_tag="PLT08_16900"
     CDS             complement(<1..398)
                     /locus_tag="PLT08_16900"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013434.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HNE06207.1"
                     /translation="MKQHIYWHTTVQMPEGNNLTPIPTKADVAIIGGGYTGLSAARTL
                     AKQGVSVVVLEAETIGWGASSRNGGMTLTGLKPAMQTVIKNYGRELAKELFQCSLDSV
                     NIVEQIVKEENINCGFARYGHLLTANKPKH"
     gene            528..1274
                     /locus_tag="PLT08_16905"
     CDS             528..1274
                     /locus_tag="PLT08_16905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE06208.1"
                     /translation="MEWTGFLGFFGFCIAGLAGPLSIIVLYFVIRNIWKAHAYRRDLT
                     LHGVCAPAVVLSAWNNYSSGGGKHSAANTAHVTFEVEVMPPGQAPFRAKFKEQLSIRN
                     GEWFVIGPRKEEVGKKIWVAYDPKDPKRMVIDHFDSDHEFMLKRRAFEKQDHELQNTR
                     KTGEDANAEILEIEDMKLSNIIENGFQSVMRLKLRVEPRSGTPYETETVGMFSNTGLH
                     KYTVGKKMTVKIDRADKYKVALVGPIQQEA"
     gene            complement(1331..>1764)
                     /locus_tag="PLT08_16910"
     CDS             complement(1331..>1764)
                     /locus_tag="PLT08_16910"
                     /inference="COORDINATES: protein motif:HMM:NF015892.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HNE06209.1"
                     /translation="SGKARQFAEKFPGMVTPDFTGDFDERMAQLKKIIGRKKGWTIIG
                     SSLGGLMGTVFTCQKPTHVRKLILLAPALLKDPFGSYLNLESVSVPTTVIHGTADDVV
                     PLDEAREYAEKFFTDLKYIVVDDGHRLQEAFKELDWEEILN"
BASE COUNT          460 a          367 c          437 g          500 t
ORIGIN      
        1 taatgttttg gcttgttcgc cgtcaacaag tgaccatacc ttgcaaagcc gcagttgata
       61 ttctcttctt tgacgatctg ttcaacaata ttgaccgaat ccagtgaaca ctgaaataat
      121 tcctttgcca attcccgccc ataatttttg ataaccgtct gcatggcagg ctttagtcct
      181 gtgagggtca tgccgccgtt gcgggagctg gcaccccagc cgatcgtctc tgcttcaagt
      241 acgaccacac tcacaccttg ttttgccaag gttcgtgccg cgctcaaacc cgtgtacccg
      301 ccgccgatga tcgccacgtc cgctttggtg gggatggggg taaggttatt gccttcgggc
      361 atttggacgg tggtatgcca gtagatgtgt tgtttcatga gtcggctccg ttgtgtgtta
      421 aggaatagga caattgtgcc atattttaag tcaccttaat cagatattat cactcctaat
      481 attattagtc gttctataaa ttttcattca aataaataga ggttaacatg gagtggacgg
      541 gttttcttgg cttttttggt ttttgcattg ctggattggc aggtccttta tcaattattg
      601 tgctttactt tgtaattagg aatatttgga aagcacatgc atatcgtcgc gatttgacct
      661 tgcatggagt ctgtgcccca gcagtagttc tttcagcatg gaacaattat tcatctggcg
      721 gcggaaaaca tagtgcggct aatacagccc atgttacttt tgaagttgaa gtgatgcctc
      781 ctgggcaagc tccgtttaga gctaaattta aggagcagct ctctattcga aatggtgaat
      841 ggttcgttat aggacctcgc aaagaagaag tgggcaagaa aatctgggtg gcatatgacc
      901 ctaaagatcc aaagcggatg gtgattgacc attttgactc tgaccatgag tttatgttaa
      961 agcgtcgggc atttgaaaaa caagaccatg aattgcagaa tactcgcaag actggtgaag
     1021 atgcaaatgc tgagatcctt gagattgaag atatgaagtt atccaatatt attgagaatg
     1081 gtttccaaag tgtaatgcgc ttaaagttgc gcgttgaacc cagaagcggg acgccttacg
     1141 aaacagaaac agtagggatg tttagtaata cggggttaca taaatacact gtcggtaaaa
     1201 agatgactgt gaaaattgac cgagcagata aatacaaggt tgctctggtg ggtccaatac
     1261 agcaggaagc ataagggcta tattacgcct attaaatcaa acaccgccaa aataaatggc
     1321 ggtgtttgat ttaattcaat atctcttccc aatctaattc tttaaacgcc tcttgcaagc
     1381 ggtgaccatc gtccacgacg atgtatttca agtcggtgaa gaatttctcg gcgtattcgc
     1441 gcgcctcatc gagcggaacg acgtcatctg ccgtcccgtg gataacggtt gtggggacag
     1501 aaacagactc gagattaagg taagagccaa aggggtcttt gagcaaagcc ggagcaagca
     1561 gaatcaactt gcggacgtga gtcggttttt ggcaggtgaa gaccgttccc attaatccgc
     1621 ccaatgatga gccgatgata gtccagcctt ttttgcgacc gatgattttt ttgagttgtg
     1681 ccatgcgctc atcgaaatcg ccggtgaagt cgggggtgac catgccgggg aatttctctg
     1741 cgaactgacg ggctttgccg cttt
//
LOCUS       DAOQJE010000821         1763 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_58996_length_1763_cov_6.099532, whole genome shotgun sequence.
ACCESSION   DAOQJE010000821 DAOQJE010000000
VERSION     DAOQJE010000821.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1763)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1763)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1763
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_58996_length_1763_cov_6.099532"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..404)
                     /locus_tag="PLT08_16915"
     CDS             complement(<1..404)
                     /locus_tag="PLT08_16915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HNE06210.1"
                     /translation="MKPEGKRYSTVVGNKTVTIETGRLAGQAGGALTLGIDEAIVFAS
                     ATMGGVRDGIDFFPLSVEYEERLYAGGKIPGSFFRREGRASTESILTARLTDRPLRPL
                     FQDGMRNEVQVIMYSFSSDGVNPLDILAINAAS"
     gene            complement(584..856)
                     /gene="rpsO"
                     /locus_tag="PLT08_16920"
     CDS             complement(584..856)
                     /gene="rpsO"
                     /locus_tag="PLT08_16920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011275926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HNE06211.1"
                     /translation="MPLAKEVKTKAIEDFHRHEKDTGSPEVQVAILTNRITQLTEHLR
                     NNKQDQSCRRGLLKLVGQRRRLLTYLRQSNYQSYLSVTERLNLRRK"
     gene            complement(943..1446)
                     /locus_tag="PLT08_16925"
     CDS             complement(943..1446)
                     /locus_tag="PLT08_16925"
                     /inference="COORDINATES: protein motif:HMM:NF024273.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HNE06212.1"
                     /translation="MDKKEIEFQLEDTQRRFIELVNSIPEENYSLPTDNPAWSVGDIL
                     FHITLGPRALALEIWMTAHASGFFGFMMRHFPSRLFNAINAWFGRGRSQRVSRQSLLK
                     AYGKAHAVIMSELRRTREEDLVKSVVYPPEFVSDLAGEVTPERLFRYVTGHFEAHEGQ
                     LRRDSLV"
BASE COUNT          412 a          446 c          446 g          459 t
ORIGIN      
        1 gatgctgcgt tgatagcaag gatgtcgagc gggttcacgc cgtcagacga gaacgagtac
       61 atgatgactt gcacttcatt gcgcatgcca tcctgaaaga gtgggcgtag aggtcggtca
      121 gtgaggcgag cggtgaggat cgactcggta gaggcgcgcc cttctcggcg gaagaaagag
      181 ccgggaattt ttcctccagc atacaatctt tcttcgtatt ccacgctcaa ggggaagaag
      241 tcgattccgt cgcgcacgcc gcccatggtg gctgaggcga acacaatggc ttcgtcgatt
      301 ccaagcgtaa gagcgccgcc agcctgcccg gctaagcgac ccgtttcgat ggttaccgtt
      361 ttgttaccaa cgacggttga gtaacgttta ccttcgggtt tcatatttaa ttgtctccta
      421 aaatcttttg ggcgagtcac ggtcgcccct gaaagctccc gcctcggggt cagggactga
      481 agagtgggaa caacagagtt attcgcgcta ttcaattcct gacggcgggt ttacacaatg
      541 taggggcaca ggttcactgt gcccctacgg ggtttcgttc tatttacttg cggcgcaagt
      601 tcaaacgttc agtgacactg aggtacgact gatagttgct ctggcgcagg taggtgagta
      661 agcggcggcg ctgacccacc aacttgagca aaccacggcg gcaggactga tcctgcttgt
      721 tgttgcgcaa gtgttcggtc aactgagtaa tgcggttggt caaaattgcg acctgaactt
      781 caggtgagcc ggtgtctttc tcgtggcggt gaaaatcttc aatcgccttt gtcttgactt
      841 cttttgcgag gggcatgacg tatgcgtctc ctttctaata atattgcagg tctccgtgcc
      901 gaaagctttt aaaccgtcgg taggaccagt cacggggatg gattatacca gagaatcccg
      961 ccgtaattgc ccctcatgcg cctcgaaatg ccctgtcaca taccgaaaaa gacgctctgg
     1021 cgtgacctct ccagccaggt ctgacacaaa ctcaggcgga tacaccaccg atttgactaa
     1081 atcctcctcc cgcgttcgcc tcaattcaga cattatgact gcatgtgcct tcccataagc
     1141 cttgagcaaa ctctgacggc tgactcgttg gctccgtccg cgcccgaacc aggcattgat
     1201 cgcgttaaag agccgtgaag ggaaatgccg catcataaag ccaaagaatc ctgaggcgtg
     1261 tgctgtcatc caaatttcca gcgccaatgc ccgcggaccc aaggtaatgt gaaagagaat
     1321 atctcccaca ctccaggctg ggttgtctgt agggagagaa tagttttctt caggaatcga
     1381 attaaccagt tcaataaatc gacgttgcgt atcttctaat tgaaattcaa tctctttttt
     1441 atccattttg ctttccatct tgcatttgat ttttggctgt gatacgcttt gcttatctaa
     1501 ttctaatctg aaaggaggta cttcttgaat gactatcatt aatcatgaaa caacaaatct
     1561 atgcgtgcct ccccgcgggt gggttgggta attcattccc attttgaaac cttgccgcag
     1621 gcgcgctgat gccctgcggt tttttattgc catagggcgg actattgata ccgaacatta
     1681 agctaccata ttcaggagaa atctatgtcg tctgtcattt cgatctcgaa actggcaaaa
     1741 cattaccaag tccccgaacg tga
//
LOCUS       DAOQJE010000822         1763 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_59004_length_1763_cov_4.521663, whole genome shotgun sequence.
ACCESSION   DAOQJE010000822 DAOQJE010000000
VERSION     DAOQJE010000822.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1763)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1763)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1763
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_59004_length_1763_cov_4.521663"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..757)
                     /locus_tag="PLT08_16930"
     CDS             complement(<1..757)
                     /locus_tag="PLT08_16930"
                     /inference="COORDINATES: protein motif:HMM:NF023568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HNE06213.1"
                     /translation="MKRSVKFILGAVVGILVLGAAGILWKTRQEAYNLLHAPMADREL
                     PDELPSDYNMQYEDVTVTNPDGMKLVGWFIPSENGAVIIMQHGYKSTRSEMLNEAEMM
                     HRHGYGVLLTTVRAHDESDGELITLGYYEMADLEAWYQYLISRDDIDPEKIGILGNSY
                     GGMLSIQYAAQNQNIKAVVADCAFSSMKDTVSTSVRHFTGLPDFPFVPLIVFWAENMT
                     GISIEDIDATKWIPNISPRPVFLMQGGKDTVISP"
     gene            complement(864..>1763)
                     /locus_tag="PLT08_16935"
     CDS             complement(864..>1763)
                     /locus_tag="PLT08_16935"
                     /inference="COORDINATES: protein motif:HMM:NF012311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HNE06214.1"
                     /translation="VVGQSDSGVDGNHPAIEKQYRGYNGSDDYNWFDPWDGTTSPNDE
                     GGHGTHTMGTILGNNGIGVAPDAQWIGCVNLDRNLANPALYLDCMQFMLAPFPIGGDP
                     FVDGDPTQAAHVMNNSWGCPSIEGCDPNALLYAANHLRDAGIFVVVSTGNDGPNCETV
                     EAPLSLYDSVFSVGAIDQSGDLARFSSRGPVTADGSGRIKPDIVAPGVDVFSSLPENS
                     YGSNSGTSMAGPHMVGVVALIWSAQPELIGDIDATEQLIIDTAKPYMGDRSLGCFSGD
                     IPSNAFGFGVVDVYEAVKEALKK"
BASE COUNT          477 a          456 c          397 g          433 t
ORIGIN      
        1 tgggcgaaat gaccgtgtct ttgccgcctt gcattaagaa caccgggcgc ggactaatat
       61 taggaatcca ttttgttgca tcaatatcct cgatgctgat gccggtcatg ttctctgccc
      121 aaaagacgat aagtggcacg aatggaaaat cgggtaagcc cgtgaaatga cgcacactgg
      181 tagaaactgt atccttcatg gaagagaaag cacaatctgc cacgaccgct ttgatatttt
      241 gattttgtgc cgcatattga atagaaagca tgccaccata agagttgccg agaatgccaa
      301 tcttttcggg gtcaatgtca tcacgcgaga taaggtattg ataccaggct tcgaggtctg
      361 ccatttcata atatccaagc gtgatgagtt ctccatcact ctcatcatgc gcgcgaacag
      421 tggtaagcag aacgccatat ccgtgccggt gcatcatctc ggcttcgttg agcatttcgc
      481 tgcgagtaga tttatatccg tgctgcataa tgatgaccgc cccattttca gaaggaataa
      541 accaccccac caatttcata ccatctgggt tggtaacagt gacatcttca tattgcatgt
      601 tgtaatcaga aggcagttca tcgggcagtt cacggtctgc catgggagca tgcaataagt
      661 tatatgcttc ctgtcttgtt ttccataaga tccctgctgc ccctaataca agaattccaa
      721 cgaccgcccc taatataaac ttgacgctac gcttcataaa ttcatccttt ggcaagagag
      781 atgcaagcaa tgggtcaatt ttgccacaga gttaaaaagg cgcagagagt aaatttctct
      841 gcgccttttt gaattcatga aatttacttt ttcaaggctt cttttacagc ttcgtacaca
      901 tccaccacgc caaagccaaa ggcgttgctg ggaatatcgc cgctaaaaca acctagcgaa
      961 cgatccccca tataaggctt ggcagtatcg ataattagct gttctgtcgc atcaatatca
     1021 ccgatcaact caggctgggc agaccagatc aacgctacca cccccaccat atgcggaccc
     1081 gccatcgatg taccgctatt ggagccgtac gaattttcgg gtaaggatga aaacacatcc
     1141 acacctggtg caacgatatc gggtttgatg cgcccagagc cgtccgcagt gacaggtcca
     1201 cggctgctga acctagcgag gtctccagat tggtcaattg cacccacaga gaagactgaa
     1261 tcataaagag acagaggagc ctctaccgtt tcgcagttcg gtccgtcatt gccggtagaa
     1321 accaccacaa aaatgccggc gtcacgcaaa tggtttgcag cataaagcaa agcattcggg
     1381 tcacagcctt caatcgaagg gcagccccat gaattgttca tgacatgcgc ggcttgagtt
     1441 gggtcaccat ctacaaaggg atcaccgcca atcgggaagg gcgcgagcat gaactgcata
     1501 caatctaaat acaaagctgg gttagcaaga ttacggtcaa ggttcacgca cccgatccat
     1561 tgtgcatcag gagcaacacc gatgccatta tttccaagga tcgttcccat agtgtgagtg
     1621 ccgtgcccgc cttcatcatt cggtgatgtt gtgccatccc acgggtcgaa ccagttgtaa
     1681 tcatcactgc cgttataacc acgatactgt ttttcgatcg caggatgatt cccatctaca
     1741 cccgagtcag attgccctac tac
//
LOCUS       DAOQJE010000823         1762 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_59057_length_1762_cov_6.626245, whole genome shotgun sequence.
ACCESSION   DAOQJE010000823 DAOQJE010000000
VERSION     DAOQJE010000823.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1762)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1762)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1762
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_59057_length_1762_cov_6.626245"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..666
                     /locus_tag="PLT08_16940"
     CDS             <1..666
                     /locus_tag="PLT08_16940"
                     /inference="COORDINATES: protein motif:HMM:NF012296.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HNE06215.1"
                     /translation="EEANRAATEFMEMIGRLAAERRINPQDDLITELVQVENQGDKLT
                     EDELRATAIFLLNAGHEATVNGSSLGLRALFQKPEQLKALKEAVAQGNTSLLKTGVDE
                     MLRYETPLPMFERYVLEDMDFNGVKLERGMEVALMYISGNRDEARFTQPNELILSRED
                     NPLLTFGLGTHYCLGAPLARLELQVLFSVLFTRCPNIRSAGEAEFSSGFVIRGLNKLP
                     IEF"
     gene            817..1656
                     /locus_tag="PLT08_16945"
     CDS             817..1656
                     /locus_tag="PLT08_16945"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HNE06216.1"
                     /translation="MRKTVLITGSTTGIGKAGALELGRRGWQVFVHARSEERGKPVLD
                     ELKNSTPNANFSLVTGDVSSMEQVKSLAKQVKEQTNTLDVLWNNAGLMLNERQVSVDG
                     WEKTMAINHLAPFVLTRELIPLIESVQGRVITTSSGAHYFGSFNWDDWMDEKSKYNSF
                     KIYSKSKLANVLFTRELAKRIDAKGITAHCFHPGMVRTDFGKQTRGVPSQGSWYPRWM
                     EALFTIDTEAGADTGVFLAENPSTKDSNGKYWFKRKVRRTNRFDNDQNAAKLWELSEK
                     ATN"
BASE COUNT          477 a          430 c          431 g          424 t
ORIGIN      
        1 gaagaagcca atcgcgccgc caccgaattc atggaaatga tcggcaggct cgccgccgaa
       61 cgccgtatca atccgcaaga tgatctcata accgaattgg tgcaggtgga aaatcagggc
      121 gataagctca ccgaagatga actgcgcgcc actgccatct ttttgctgaa tgccgggcat
      181 gaggctacag tcaatgggtc gtcgcttggg ttgagggcac tcttccagaa acccgagcaa
      241 ctcaaggcgt tgaaagaggc ggtcgctcaa ggcaacacat ccctgctcaa aactggcgtc
      301 gacgaaatgt tgcgctacga aactccgctg ccgatgtttg agcgttacgt cctcgaagat
      361 atggacttca acggcgtcaa gctggagcgc ggcatggaag tggcattgat gtacatttcg
      421 ggcaatcgcg atgaagcccg cttcacacaa ccgaatgagt tgatcctgtc tcgtgaagat
      481 aaccctctgc tgacgtttgg tctcggcacc cattactgcc tcggcgctcc gctcgcacga
      541 ttggagttgc aggtgctgtt cagtgtgttg ttcacacgct gcccgaacat tcgctcggcg
      601 ggagaagcgg aattttcttc cggctttgtc attcgtgggt tgaacaaatt acccatcgaa
      661 ttttaatgac accttgtgtc aacaaaaaag cagccccaaa aggctgcttt tttgttgcaa
      721 acctattctc acaaaattat taattattca gagaaagatt tttttcttac attcaatcta
      781 cacgaaggtg aaaattcatc gcaaaaggaa attattatga gaaaaacagt tttgattaca
      841 ggctcgacca cgggcattgg taaagcaggc gcattggaat tgggacgtcg cggttggcag
      901 gtatttgtcc atgcccgttc ggaggagcgc ggaaagccgg tgttagacga gttgaagaac
      961 agcacgccga acgctaattt ctcacttgtc actggtgatg tatcttcgat ggaacaggtc
     1021 aagtctcttg ccaaacaggt caaggaacag accaatactt tggatgttct ttggaataac
     1081 gcaggactta tgctgaacga acgccaggtc agcgtggatg gatgggaaaa gaccatggcg
     1141 attaaccatc tcgctccgtt cgtattgact cgtgaattga tcccgctcat cgaatccgtt
     1201 caaggcagag tcatcaccac cagttctggc gctcattact ttgggtcttt caactgggat
     1261 gattggatgg acgagaagag caaatataac tcgttcaaaa tctacagcaa aagcaaactt
     1321 gccaatgtcc ttttcacccg cgaattagcg aagcggattg acgcaaaagg catcacagct
     1381 cattgtttcc acccaggcat ggtgcggact gacttcggca agcaaactag aggagtgcca
     1441 tctcaaggtt cttggtaccc acgctggatg gaagcactat tcactattga tactgaagcc
     1501 ggtgccgata ctggtgtatt tcttgcagaa aacccatcta ccaaagacag caatggaaaa
     1561 tattggttca agcgcaaagt ccgccgcaca aatcgcttcg acaatgatca gaatgccgct
     1621 aaactttggg aacttagcga aaaagcaacc aactagttat tatgaaatca tccccgtcag
     1681 gaatgcgggg atgatttcat tgttaagtta tactcgcgcc catgtcttca caaaaacccg
     1741 ctctcgaccc catgatctcg tc
//
LOCUS       DAOQJE010000824         1762 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_59066_length_1762_cov_4.392501, whole genome shotgun sequence.
ACCESSION   DAOQJE010000824 DAOQJE010000000
VERSION     DAOQJE010000824.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1762)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1762)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1762
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_59066_length_1762_cov_4.392501"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(227..523)
                     /locus_tag="PLT08_16950"
     CDS             complement(227..523)
                     /locus_tag="PLT08_16950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HNE06217.1"
                     /translation="MKDELFNDLLTSVREGGAILRGEKKPSRAFKITPPNIKRIRAGY
                     KLSQEDFASLLGISAATLRNWEQGRRSPQGPARVLLQVAARYPDIVWDVVKPTK"
     gene            complement(520..840)
                     /locus_tag="PLT08_16955"
     CDS             complement(520..840)
                     /locus_tag="PLT08_16955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HNE06218.1"
                     /translation="MMIIVETQIFTRQVLLLLSDEEYRLLQSVLANRPDAGVVIPSSG
                     GLRKMRWGMTGRGKRGGVRVIYYWAVKQERILMLLIYPKNVKDDMTQEQLKILRKIVK
                     EEFS"
     gene            885..>1762
                     /locus_tag="PLT08_16960"
     CDS             885..>1762
                     /locus_tag="PLT08_16960"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HNE06219.1"
                     /translation="MTITKNYFNPSISRLILWLPVLLFLLSTFFFPLFKILTLTFNLE
                     TLTNLNNLRITYHALLFTFYQATLSTLLTFALGLPAAILFSKFNFHGKSLLRALTAVP
                     FMLPTVVVAAAFNSIFSPRGLFSFLFPSFTQSVHPSPFILILITHTFYNTSIVIRIVG
                     NAISRLDPKLEASARVLGADTFHVWKDVILPILRPSLLAAALLVFMFDFTSFGVILLL
                     GGSQFATLEVEIYIRALQLPNLNLAALLSVIQLIFTLIFSIFYSRTINQVQTQTAPRF
                     STSRPPKTIQEKIFVAA"
BASE COUNT          398 a          553 c          312 g          499 t
ORIGIN      
        1 aatatgttcg tctaccccgt taaccagtcc gcgcaacttc cggatgtgtt cacaaaatac
       61 gctcaagttg ctgaacagcc tgcgaatatt tcctataatg aaatcgcctc gaaccgtgac
      121 aagtggatcg aggcgtgggc agaaattatg aagtaggggc gacccgtcaa catcttttag
      181 aaccttgttg tgcaaaggga gggtcgccct ttttgagaaa atatgattat tttgttggct
      241 tcacaacatc ccagacaata tccgggtaac gtgccgctac ttgtaacaac acccgcgcag
      301 gaccttgcgg ggaacgcctg ccttgctccc aatttcgcaa tgtggcggcg ctgataccga
      361 gcaacgaagc aaaatcttct tgcgaaagtt tatatcctgc gcgaatccgc ttgatgttgg
      421 gcggggtgat cttgaaggcg cgggagggtt tcttctctcc gcgcaagatt gccccgcctt
      481 cgcggacact tgtcagaagg tcgttgaata attcatcttt catgagaact cctctttcac
      541 gatctttcgc aggattttta gttgttcctg cgtcatatcg tcttttacat tctttgggta
      601 aatcaggagc atcaaaatgc gttcctgttt taccgcccaa taatagatga cacgcacacc
      661 gccgcgtttt cctcgccccg tcatgcccca gcgcattttc cgtaacccgc cactggatgg
      721 aatgactaca ccagcatcgg gtcgattggc aagaaccgat tgcaataaac ggtattcttc
      781 atcgcttaag agaagcagaa cctgccttgt aaatatctgc gtttcaacga taatcatcat
      841 taaccattat acgccattgg cgtagttagt ggaagagtga aaatatgacc ataaccaaaa
      901 actatttcaa cccttcaata tcacgcttaa tcctttggct tcctgttctc ctctttcttc
      961 tgtccacttt cttctttccc cttttcaaaa ttcttaccct cacgttcaac ctcgaaaccc
     1021 tcaccaacct caacaactta cgcatcacgt atcacgcatt actcttcacc ttctaccaag
     1081 ccaccctatc cacactcctc accttcgccc ttggactccc cgctgccatc ctcttctcca
     1141 aattcaactt tcacggcaag tccctattgc gtgcgttgac cgctgtccct ttcatgctgc
     1201 cgacggttgt cgttgccgct gccttcaatt caatcttttc tccacgcgga ctcttttctt
     1261 tcttatttcc ttctttcacg caaagcgttc atccttcgcc cttcatcctt atcctcatca
     1321 cccacacctt ctacaacacc tctatcgtca tccgcatcgt tggcaatgcc atctcacgtc
     1381 ttgacccaaa actcgaagcc tctgcccgtg tcctcggcgc ggacaccttc cacgtttgga
     1441 aagatgtcat cctcccaatt ctgcgtccat ccctgcttgc cgctgccctg ctcgtcttca
     1501 tgttcgactt caccagtttc ggcgtcatcc tcctgctcgg cggatcgcaa ttcgccaccc
     1561 tcgaagtgga aatctacatc cgcgctctgc aacttcccaa cctcaacctc gccgcgctat
     1621 tatccgtcat ccaactcatc ttcaccctca tcttttccat tttttactca cgaaccatca
     1681 accaagtcca aacacaaact gccccgcgtt tttccacttc tcgtccaccc aaaacaatcc
     1741 aagaaaaaat ctttgtcgct gc
//
LOCUS       DAOQJE010000825         1760 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_59206_length_1760_cov_5.357185, whole genome shotgun sequence.
ACCESSION   DAOQJE010000825 DAOQJE010000000
VERSION     DAOQJE010000825.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1760)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1760)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1760
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_59206_length_1760_cov_5.357185"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1399
                     /locus_tag="PLT08_16965"
     CDS             <1..1399
                     /locus_tag="PLT08_16965"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNE06220.1"
                     /translation="VSHDRYFLDKVSTEILEMLPGAYEIYKGNYKAYLKQREERIARR
                     QEVFESEKEKLLKEVDYIKKHIAGQNTLQAKGKLKRLSRLVQAIEQIGFDAALNQKWS
                     ETADEVSVTTSSFSAEEAERRVRALRSPVRTLPNLHLRLGSEKRSGDLVIRTKNLSVG
                     FPDKFLFKSPDIELRRQDCAALIGPNGAGKSTFLKTILGQFEPLGGEVELGSSLHIGY
                     FAQAHEGLDPEKTLLQEIDSVMPHWQPGQIRDYLGKYLFSGDDVYKKVGMLSGGERGR
                     LALSKLALQDTNLLLLDEPTNHLDIPSQEVLEAVLEDYTGTILLISHDRYLVDALATQ
                     IWEVNPDAGELIAFKGTYSELRAEREQKEYEAFMAAQKNNDTRTSGSDNRGSTNESRK
                     SKNANTKEERRKIAQLQELENKIAELESTLANLSSQLESPMVKPDEVIKIGNEYNRVQ
                     AEMDEKLAEWERMQI"
     gene            1454..>1760
                     /locus_tag="PLT08_16970"
     CDS             1454..>1760
                     /locus_tag="PLT08_16970"
                     /inference="COORDINATES: protein motif:HMM:NF016115.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004061 - arylformamidase activity
                     [Evidence IEA];
                     GO_process: GO:0019441 - tryptophan catabolic process to
                     kynurenine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclase family protein"
                     /protein_id="HNE06221.1"
                     /translation="MLTYTYIDLSHTIHDGLVTYKGLPATIVCDYLTREASRGRYAPG
                     TEFHIGKIEMVTNTGTYVDSPFHRYADGKDLSEMELEHFVDLEGIVIRADYRKGLAVT
                     "
BASE COUNT          504 a          421 c          446 g          389 t
ORIGIN      
        1 cgtttcgcat gaccgttatt ttttggataa ggtcagcacc gaaattttgg aaatgctgcc
       61 cggcgcgtat gagatatata agggcaatta caaagcgtat ctgaaacaac gtgaagaacg
      121 cattgcccgc aggcaagaag tgttcgagag cgaaaaagaa aaactgctca aagaagtgga
      181 ttacatcaaa aaacacattg ccgggcagaa caccttgcaa gccaaaggca agctgaaacg
      241 actctcacgc ctggttcaag cgattgagca gatcggtttt gatgcggcat tgaatcaaaa
      301 atggagtgag actgccgacg aagtcagtgt caccacttct tcgttcagcg cagaagaagc
      361 ggaacgccgt gtgcgtgcac tccgctctcc ggtgcggacg ttgcccaact tgcatctacg
      421 cctcggctct gagaagcgct ctggtgactt ggtcattcgt accaaaaact tgagtgtggg
      481 cttccctgat aaattcctct tcaagtcgcc agacattgaa ctgagaagac aagactgcgc
      541 cgcattgatc ggtccgaatg gcgcaggtaa atccacattc ttgaagacta ttctcggtca
      601 gttcgagcct ttgggtggag aagtagaact gggttcaagt ttacacattg gttactttgc
      661 ccaagcgcat gaagggctcg acccagaaaa gactttgctg caagagatcg actcggttat
      721 gccgcattgg cagccgggac agatccgcga ttatttgggc aagtatctct ttagcggcga
      781 cgatgtgtat aaaaaagtgg gcatgctctc cggcggcgaa cgcggacgtt tggcgttgtc
      841 gaaactcgcg ttgcaagata cgaatctctt gttattggat gaaccgacga accaccttga
      901 tattccttca caggaagtct tagaggctgt actggaagat tacaccggga ctattttgct
      961 catctcgcat gaccgttacc tcgtcgatgc attggcaacc caaatatggg aagtcaaccc
     1021 cgatgccggt gagttaatcg cattcaaagg aacctattct gagctaagag ccgaacgcga
     1081 gcaaaaggaa tatgaagcgt tcatggcagc gcagaaaaat aatgacactc gaacttcggg
     1141 aagtgacaat cgaggatcta ccaacgaatc tcgaaaatcc aaaaatgcga acacgaagga
     1201 agaaagacga aagattgccc agcttcaaga actggaaaac aagatcgccg agttggagtc
     1261 tacgctggcg aatttgagct cgcagttgga gagtcccatg gtcaagccag atgaagtcat
     1321 caagatcggc aatgaatata accgcgtcca agccgagatg gatgagaaac tggcagagtg
     1381 ggaacggatg caaatttaat catgagcatt attgggagct aagccctcaa caatgcaacg
     1441 agaggaacaa cacatgctca cctacaccta cattgacctc agccacacca ttcacgatgg
     1501 tctcgtcact tacaaaggat tgcctgctac catcgtctgc gactacctca ctcgtgaagc
     1561 ctcgcgtgga cggtatgccc ccggcacaga atttcacatc ggcaaaattg aaatggtgac
     1621 caacaccggc acttatgtgg atagtccctt tcatcgttat gctgatggca aagacctctc
     1681 cgaaatggaa ttagagcatt ttgtcgacct tgaaggcatc gtcatccgcg cggactatcg
     1741 caagggattg gctgtcaccg
//
LOCUS       DAOQJE010000826         1756 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_59454_length_1756_cov_6.902998, whole genome shotgun sequence.
ACCESSION   DAOQJE010000826 DAOQJE010000000
VERSION     DAOQJE010000826.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1756)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1756)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1756
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_59454_length_1756_cov_6.902998"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..860)
                     /locus_tag="PLT08_16975"
     CDS             complement(<1..860)
                     /locus_tag="PLT08_16975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Clp protease N-terminal domain-containing
                     protein"
                     /protein_id="HNE06222.1"
                     /translation="MMRFDRFTERAQEAAQRAAEIIQRYGHNQIDTEHILLALIEQPG
                     GVIPQILEKLNVSAQALTERLDATLRASPKANIFGGGAGQIFITPRVKRIIDLANEEA
                     NRLKDEYISTEHIFLAILTERNTPAARILESAGLTRDRVYDAIQDLRGGQRVTDPQAE
                     TRYRTLEKYSRDLTQLAREGKLDPVIGRDNEIMRLIQILSRRTKNNPVLIGEAGVGKT
                     AIAEGLAQKIASNDVPEILSGKRVVALDLGAMIAGSRFRGEFEERLKAVMEEVQRAKG
                     DVILMIDELHT"
     gene            1110..>1756
                     /locus_tag="PLT08_16980"
     CDS             1110..>1756
                     /locus_tag="PLT08_16980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE06223.1"
                     /translation="MKRLFPFILTTIFILQACGGNAPTEESAAAPQTANTEVAAATAD
                     VALATAEPIVHVDIPTEGTVSRASAHDNEESANSGNKDVIFGDDFEKNRFERPFTAND
                     MTYLPDLDIVDFSITSDDSFFYIRIDLVGLDLASQSLTGFYGVEFDKNGDGRAEIFLV
                     TQPPYKTEFTADNVFLFIDANGDVGSEQAAKPDASNGDGFDSLLFDLNDSIHPEDP"
BASE COUNT          421 a          484 c          405 g          446 t
ORIGIN      
        1 gtatgcaact catcgatcat caggatgaca tcacccttcg cgcgttggac ttcctccatc
       61 actgccttga gacgttcttc gaactcgcct ctaaatctgg acccggcgat catagcgccc
      121 aaatcgagcg ctaccactct cttgccggat agaatctcgg gcacatcgtt gctggcgatc
      181 ttctgcgcca aaccttctgc aatggctgtc ttccccacac ccgcttcacc gatcaaaacc
      241 ggattgttct tcgtacggcg agacagaatc tgaatcaggc gcatgatctc attatcgcgc
      301 ccaatgaccg ggtcaagctt accttcacgg gcaagctgcg tcaggtcacg cgaatatttt
      361 tcaagggtgc ggtaacgcgt ctccgcttgt ggatcggtca ctcgttgacc accgcgcaga
      421 tcctgaatgg catcatacac acggtcacgg gttaatcctg cagattccaa aatgcgagcc
      481 gcaggggtat tacgttcagt gagaatcgcc aaaaagatat gttcggttga aatatattca
      541 tctttgagac ggttggcttc ttcgttggca aggtcgataa tccgcttgac gcgcggggtg
      601 atgaaaatct gtcccgctcc gccgccgaag atgttcgcct tggggctggc gcgcagggtg
      661 gcatccagcc gttcggtgag cgcttgagcg ctgacattca atttttcaag aatttgggga
      721 atcactccac caggctgttc aatcagcgcc agcagaatgt gttcggtatc aatttgattg
      781 tgcccatagc gttggataat ctccgcagca cgctgggcgg cttcttgtgc tctctctgta
      841 aatcggtcaa atcgcatcat aggggtatcc tccggacaga ccacagacca tggatcgcag
      901 accatggttt tatggtctat cgcccattgt ctatcgtcca cccaatgctg gcattataac
      961 aaacgccgta tcggcgaatt gtcagtatgg cataagaaga gtgttagatt tcggcgacca
     1021 tcactaaagt aataccctat tccttacttt tttgggtgta gcgccagaaa agaataaact
     1081 attatctcca atataccgtt aggagactca tgaaacgcct tttccccttt atcctcacta
     1141 caattttcat tttgcaagcc tgtggcggaa atgcccccac agaagaatca gccgccgctc
     1201 cccaaactgc caatacagag gtcgctgctg ccacagcaga cgttgcacta gctactgcag
     1261 aaccgattgt gcatgttgac atcccaaccg aaggtacggt gagccgagcc tcagctcatg
     1321 acaacgaaga aagcgccaac tctggaaata aagatgtgat ctttggtgat gacttcgaga
     1381 aaaatcgttt tgaacgcccc tttacagcca acgatatgac ctatctcccc gatctcgata
     1441 tcgtggattt ctccatcacc agtgacgact ccttctttta catccgcatc gatctggtcg
     1501 gacttgatct tgccagccag tctctgaccg ggttctacgg tgtggagttt gacaagaacg
     1561 gtgatggtcg cgccgaaatt tttcttgtaa cccaaccgcc ttataaaaca gaattcactg
     1621 ctgataatgt ctttctcttt attgatgcca atggagacgt aggcagcgaa caagccgcca
     1681 aacccgatgc ttccaacggc gatggtttcg actctctgct ctttgacctg aacgatagca
     1741 tccaccccga agaccc
//
LOCUS       DAOQJE010000827         1746 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_60068_length_1746_cov_6.830278, whole genome shotgun sequence.
ACCESSION   DAOQJE010000827 DAOQJE010000000
VERSION     DAOQJE010000827.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1746)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1746)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1746
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_60068_length_1746_cov_6.830278"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(127..1119)
                     /locus_tag="PLT08_16985"
     CDS             complement(127..1119)
                     /locus_tag="PLT08_16985"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HNE06224.1"
                     /translation="MAFNTLFKKDSLNEGGKLTERQRRFAYTSLAPILLYMGVFTLFP
                     IVWAVLMSFFDYTPIREGSGFLGLGGANPFIAIDNYIELFTENTKPAEVFRLALKNTF
                     IFTALVLPLNLIITLPLAVLLESIGERFKTLFRAIYFLPTISSSVALVIIWSYMYAPG
                     WGLLSIMFKAVGLVPPKSWLQDPSQVYFGVPLAMICVVIAHVWQDFGYNLVIFVAALQ
                     SIPKTLREAARVDGANTWQEFWLVVVPLLSRTVLLTSVLTVISSLQVFVIFQLLTRGG
                     PQNQTQTLLLSIYENAFKFANNLGLSAAMSMILFIIILTLTVIQFRILRTEWEY"
     gene            complement(1181..>1746)
                     /locus_tag="PLT08_16990"
     CDS             complement(1181..>1746)
                     /locus_tag="PLT08_16990"
                     /inference="COORDINATES: protein motif:HMM:NF013697.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HNE06225.1"
                     /translation="QKDGVVDPIIFNAESEWVGDSFAQGHNAIGVLGSWYAGDAKIGY
                     PDLVFDYVSLPPMMGTEHKFVSVGGWGYVVGKSTQHPDIAWQLAAFLGANEANVMYFN
                     TTSATIPAMKSVAADAKYVEAVPFASAVLPILDAGAYQGNLTDPDQLAYEIIYPTILD
                     AVQGNQTAEEAANSIHEAANAMVDSAQ"
BASE COUNT          447 a          412 c          503 g          384 t
ORIGIN      
        1 gaagagacga tgatgtaaaa gaacggcagg agggtgccga tcagcccgac gatcaagacg
       61 gcatatgcgc ctgatttgcc gagtacgaaa ccgatggaag gctttttgtt tgagggactg
      121 gtcatcttaa tactcccatt cagtgcgcag aatgcggaat tgaatcacgg tcagggtcag
      181 gatgatgatg aataagatca tcgacatggc tgccgacaac cccaggttat tggcgaattt
      241 gaaggcgttc tcgtaaatgc tcaatagcaa agtttgagtc tggttttgag gtccgccgcg
      301 agtgaggagt tggaagatga cgaacacttg cagcgaagag atcaccgtca gaacgctggt
      361 cagtaacacg gtccgactga gaagcgggac gaccacgagc cagaattcct gccaggtgtt
      421 ggcaccatct acgcgggcgg cttcgcgtaa cgtcttcggg atggattgca aggcggcgac
      481 aaagatgacg aggttgtagc caaaatcctg ccagacgtgc gcgatgacca cgcagatcat
      541 cgccagcgga acaccgaagt agacctggct tgggtcttgc agccatgatt tgggtgggac
      601 gagtcccacg gctttgaaca tgatgcttag caacccccaa cctggtgcat acatgtacga
      661 ccagatgata acaagcgcca cagaagatga aatggtgggc agaaaataaa tggcgcggaa
      721 taaggtcttg aaacgctcgc caatggattc aagcaatacg gcgaggggaa gtgtgatgat
      781 gagattgaga ggtaatacca gcgcggtgaa gatgaacgtg ttctttaatg cgaggcgaaa
      841 gacttcggcg ggtttggtat tttctgtgaa aagctcgatg tagttatcga tggcaatgaa
      901 cggattggct ccacccaagc cgagaaagcc actcccttcg cggatggggg tgtagtcgaa
      961 gaagctcatc aacactgccc agacaatggg aaacaacgtg aacactccca tataaagaag
     1021 aatgggtgcc aggctggtat aggcaaaacg acgctgtctt tccgtcagtt tgccgccctc
     1081 gttgagggag tcttttttga agagagtgtt gaatgccatg ggcttctcca gaaatttcat
     1141 gtaggggcgg ggtcaccccg cccctacaaa ttcttaaacg ttactgcgca gaatccacca
     1201 tggcatttgc ggcttcatga atgctgttcg cggcttcttc ggcagtctgg ttgccttgaa
     1261 cggcatcaag aatggtcggg tagatgatct catatgccaa ttggtcgggg tcagtcaggt
     1321 tgccctgata ggcacccgca tccaggatcg ggaggacggc gcttgcgaat ggaacggctt
     1381 ctacatactt cgcatccgcg gcgacagact tcatcgcagg gatggtcgct gaggtcgtgt
     1441 tgaagtacat cacattggct tcattggcac cgaggaaagc agcgagttgc caggcaatat
     1501 cggggtgttg cgtgctcttg ccgaccacgt aaccccatcc gcctacggag acgaatttgt
     1561 gttccgtgcc catcatcggt ggcagggaaa cataatcgaa caccaagtca gggtaaccga
     1621 tctttgcatc acctgcatac caggaaccta atacgccaat ggcgttatgt ccctgtgcaa
     1681 atgaatcgcc gacccattca ctttcggcat tgaagatgat cgggtcaacc acgccgtctt
     1741 tttgag
//
LOCUS       DAOQJE010000828         1735 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_60803_length_1735_cov_6.613690, whole genome shotgun sequence.
ACCESSION   DAOQJE010000828 DAOQJE010000000
VERSION     DAOQJE010000828.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1735)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1735)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1735
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_60803_length_1735_cov_6.613690"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..848)
                     /locus_tag="PLT08_16995"
     CDS             complement(<1..848)
                     /locus_tag="PLT08_16995"
                     /inference="COORDINATES: protein motif:HMM:NF013180.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNE06226.1"
                     /translation="MDKPTALIIEDDRDIVALFRHVLDVAGYQTEIVLDGKDAMDRLD
                     VMRPHIVLLDLQLPRVSGIDILKRMRDDERLMRVPVVVITAYAPYADSLPVEPDLLLL
                     KPVDINQLSNLVQRLQSTQGALDEPTHDFVTGLYTLSFFNVRMTFSLERIKQSSFRRF
                     GVLFADVSQMNDLRDYLDETEMRNFLRKLADQFRVTLRPTDTMAWSPEKSLFLTLIED
                     IPTPEAPLKIAGRLRDSMKRFIEQNDRGLGIRTNVGVLLCDAEYIDIQQVLNDIDLAR
                     QQLSAG"
     gene            953..>1735
                     /gene="yqeC"
                     /locus_tag="PLT08_17000"
     CDS             953..>1735
                     /gene="yqeC"
                     /locus_tag="PLT08_17000"
                     /inference="COORDINATES: protein motif:HMM:TIGR03172.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenium cofactor biosynthesis protein YqeC"
                     /protein_id="HNE06227.1"
                     /translation="MDLIHALRLDQAKENPVVCFVGAGGKTTALFQLARQRIGKKNSL
                     VLVTATSHLGVWQIPLADHHVIVNKIEDLQLPSNGVVLVTGEIEKDRTLPVTGNALNW
                     LRAKCNSEHIPLLIEADGSRQKPVKAPATHEPPIPDFADIVVYVCGISGIGKALNDEN
                     IHRPEIFSELTNLKMNEQVSAQSLISFLTHPQGGLKNIPAHANRIALLNQADTPELQS
                     MGGAIAQKLLPTFDAVVIGSLQNNVIHLVERTAGIILAAGSSS"
BASE COUNT          522 a          417 c          359 g          437 t
ORIGIN      
        1 aaccccgcac tcaattgctg acgtgccagg tcaatatcgt tcaaaacctg ctgaatatct
       61 atatattcgg cgtcacacaa caaaacaccg acatttgtgc gaattcctaa tccgcggtca
      121 ttttgttcga tgaaacgctt catactatcg cgcaaacgcc cagcaatctt tagtggagct
      181 tcaggcgtag gaatgtcttc gatcaaggtc aagaaaaggc ttttttctgg agaccaagcc
      241 atggtgtcgg tagggcgaag cgtcacgcgg aattggtctg ccagtttgcg caagaaattg
      301 cgcatctctg tttcatccag ataatctcgc agatcattca tttgagagac atccgcaaaa
      361 agcaccccaa aacggcggaa agaactttgc ttgatgcgct ctaatgaaaa ggtcatacgc
      421 acattgaaga atgaaagcgt atatagacca gttacaaaat catgggtagg ttcatcgaga
      481 gcaccctgtg tggactgcaa tcgttgcacc aagttactaa gttgattgat atctactggc
      541 ttcagaagca gaagatcggg ctcaaccgga agactatctg cataaggggc gtaggctgta
      601 atcaccacca caggtacacg catgagacgt tcatcatcgc gcatgcgctt tagaatatca
      661 atacctgaaa ctcttggtaa ttgcagatcc aacaaaacga tatgcggacg cattacatcc
      721 aaacgatcca tggcatcttt accatccaga acgatctctg tttggtaacc ggcaacatct
      781 aacacatgcc gaaacaacgc aacaatatcg cggtcatctt caataattaa agcagtgggt
      841 ttatccataa gttcgccctt cttcgcaata tagatattat acgaccttca agcaccgttc
      901 agggtgtcat ttcaggtttt ttatcataca attacaacga aagctaatcc acatggactt
      961 aatacacgcc ctccgtcttg accaagccaa agaaaatcca gttgtttgtt ttgtgggtgc
     1021 cggtggcaaa accactgccc tctttcagct ggcacgtcaa cgaataggaa aaaagaattc
     1081 gcttgtgctt gtaacagcca ccagccattt gggtgtgtgg caaattccgc ttgcagacca
     1141 tcatgtaata gtgaataaaa tagaagattt acaattacct tcaaatggag tcgttctagt
     1201 cacaggtgaa atagagaaag acagaacctt accagtaaca ggcaatgccc taaattggct
     1261 acgcgcaaaa tgcaattctg agcatatccc tcttctcatc gaagcagatg ggtcacgtca
     1321 aaaaccggtc aaagcacctg ccacgcatga accgcctatt cctgatttcg ctgacatcgt
     1381 ggtttatgtt tgcggtataa gcggcatcgg taaagcgcta aatgatgaaa acattcaccg
     1441 ccctgaaatc ttttcagagc taacaaacct caaaatgaac gagcaagtct cagcgcaatc
     1501 tcttatttca ttccttaccc atcctcaagg tgggttgaaa aatattcctg cacatgcaaa
     1561 tcgaatcgca ctacttaatc aagccgatac tccagagttg cagtctatgg gtggagcgat
     1621 agcgcagaaa ctgttaccaa catttgatgc tgttgttatt ggctcgcttc aaaataacgt
     1681 catacatctc gtagaacgaa cagcaggaat tatccttgca gctgggtcat ccagc
//
LOCUS       DAOQJE010000829         1733 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_60946_length_1733_cov_3.834923, whole genome shotgun sequence.
ACCESSION   DAOQJE010000829 DAOQJE010000000
VERSION     DAOQJE010000829.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1733)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1733)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1733
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_60946_length_1733_cov_3.834923"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..732
                     /gene="sufB"
                     /locus_tag="PLT08_17005"
     CDS             <1..732
                     /gene="sufB"
                     /locus_tag="PLT08_17005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010921889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HNE06228.1"
                     /translation="ASVHYVEGCTAPQYTTDSFHSGVIEIIVKKGARSRYSTIQNWST
                     NVYNLVTQRAKVFENGTHEWVDANIGSKVTMKYPSCYLMEPGAHGEMLSMAFANVGQI
                     QDAGSKMVHFAPNTTSKITSKSISKSGGRASYRGLLKVYKGAKGVKSNVVCDALLLDP
                     QSRSDTYPYIEIDEDDVTIGHEASVSKVGEEQLFYLMSRGLSEEEATTMVVSGFIEPL
                     VKELPMEYAVEMNRLIQLQMEGSIG"
     gene            734..>1733
                     /locus_tag="PLT08_17010"
     CDS             734..>1733
                     /locus_tag="PLT08_17010"
                     /inference="COORDINATES: protein motif:HMM:NF013614.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SufD family Fe-S cluster assembly protein"
                     /protein_id="HNE06229.1"
                     /translation="MQEKPKVTVSRGRRADTAAKEFTFTQADVPSANGLASFRAGAWD
                     LFSKQNLPDTSLEAWRRTDLRLLPAPDFKLPKAGAFEDLPAVPEELLKPLTGEQHGGQ
                     ITLLPGGSKVDLDEALAKKGVVFTDFRTAEKNHSDLLKKLIGQIVKPDDGKFAALASA
                     LSENGVLLYVPKGVQVEQPLHSVLWGPGTDLAHFSHLLVYVEDGASVTYVHEAASPDE
                     TGHAMHAGIVEIYVGKDANLRFVELQSWGRHVWNFSHERARVDRGGNLDWIFGAVGSR
                     LTKNFSDLDLIGEGAQGRMSGFYFTDGTQHLDHDTQQNHMAPHCTSDLLFKGALKGK"
BASE COUNT          424 a          479 c          429 g          401 t
ORIGIN      
        1 gcctcggttc actatgtaga aggttgtact gctccgcaat acacgaccga ctcatttcac
       61 tctggtgtga ttgagatcat tgtcaaaaaa ggggctcgtt cgcgctattc gaccattcag
      121 aactggtcca ccaatgtgta taacctcgtg acccagcgcg ccaaagtctt tgagaatggc
      181 actcacgaat gggtcgatgc caacatcggt tcaaaggtga caatgaagta cccctcttgc
      241 tacttgatgg aacctggcgc tcatggcgaa atgctttcaa tggcatttgc taatgtcggt
      301 cagattcaag atgctggttc caagatggtt cacttcgcgc cgaacaccac cagcaagatc
      361 acttccaagt ctatctccaa gagcggcgga cgagcctcct atcgcggctt gctcaaagtt
      421 tataaaggtg ccaagggcgt caagtccaac gtcgtttgcg atgcgctctt gctcgacccg
      481 caatcccgct ccgacaccta tccctacatc gagatcgacg aagacgatgt gaccatcggt
      541 cacgaagcct cggtcagcaa agtgggcgag gaacagctct tctatctcat gagccgtgga
      601 ctcagcgaag aagaagccac aaccatggtg gtctctggct tcatcgaacc gctcgtcaaa
      661 gaactcccca tggaatacgc agtcgaaatg aatcgactca tccagttaca aatggaagga
      721 tcgattggct aacatgcaag agaaaccaaa agtaaccgta tctcgaggac gtcgcgccga
      781 cactgccgca aaggaattta cctttacgca ggcagatgtc ccttccgcga acggactcgc
      841 ttctttccgc gccggcgcct gggatttatt ctccaagcag aatctccccg acacttctct
      901 cgaagcctgg cgccgcaccg atttgcgttt attgcccgca cctgatttta aacttcccaa
      961 agcgggtgcc ttcgaagatt tgccagccgt tccagaagaa ttactcaagc cgttgaccgg
     1021 tgaacagcat ggcggacaga tcactttatt gcccggtggc tctaaagtgg acttagatga
     1081 agccctcgct aaaaagggcg tcgtcttcac tgacttccgc accgcagaaa agaatcactc
     1141 tgacttactc aagaaattga tcggtcagat cgtcaagcca gatgatggca agtttgccgc
     1201 cttggcttct gcgctttctg aaaatggcgt tcttctttat gtgcccaagg gcgtacaagt
     1261 ggaacaaccc cttcactctg tgttgtgggg acccggtact gacctcgcgc atttctcgca
     1321 tctactcgtt tacgtggaag atggcgcttc agtgacgtat gttcacgaag ccgcttcacc
     1381 cgatgaaacc ggtcatgcca tgcatgcagg aattgtcgaa atctatgtag ggaaagatgc
     1441 caacctgcgt tttgtagaat tacagtcgtg gggtaggcat gtgtggaatt tcagccacga
     1501 acgcgcccgc gtagatcgtg gcggcaacct cgactggatc ttcggcgctg taggctctcg
     1561 cctcaccaag aacttctcag acctagacct gatcggggaa ggggcgcagg gacgaatgtc
     1621 tggtttctac tttaccgatg ggacccagca cctcgaccac gacacccagc agaatcatat
     1681 ggctccgcac tgcaccagcg accttttgtt caaaggtgcg cttaaaggaa aga
//
LOCUS       DAOQJE010000830         1726 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_61439_length_1726_cov_5.546978, whole genome shotgun sequence.
ACCESSION   DAOQJE010000830 DAOQJE010000000
VERSION     DAOQJE010000830.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1726)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1726)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1726
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_61439_length_1726_cov_5.546978"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..414
                     /locus_tag="PLT08_17015"
     CDS             <1..414
                     /locus_tag="PLT08_17015"
                     /inference="COORDINATES: protein motif:HMM:NF013918.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferric reductase-like transmembrane
                     domain-containing protein"
                     /protein_id="HNE06230.1"
                     /translation="HVLIFINLDYGLAWSMIARTVFEKPYIIVGALAFLMLIPLGITS
                     FDIWKVRLGKNWKRLHQMVYVIAPLVVLHYLWSKKGDLLNFSGDIVRPTIYGLVVVVF
                     LILRIPAIRRAIASLPNRGLTLPFKKNPSPKADAS"
     gene            complement(452..682)
                     /locus_tag="PLT08_17020"
     CDS             complement(452..682)
                     /locus_tag="PLT08_17020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE06231.1"
                     /translation="MTTTTQKRQIPWYLWPFAAIWKLLAVIVEMTGRLVAMILGIVLF
                     VVGVLVSLTIVGAIVGVPLGIIGLLLFFRGMF"
     gene            803..1639
                     /locus_tag="PLT08_17025"
     CDS             803..1639
                     /locus_tag="PLT08_17025"
                     /inference="COORDINATES: protein motif:HMM:NF024853.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HNE06232.1"
                     /translation="MNTKSTRSKKSTPAVIEIEQEEPVIESTPDSEDFITFKRSHFYS
                     VLVVLAFAVGLLLGFVIWGRGSNTVTVAADPAAAANNAAAQQPSGEIAEAPVTPQPQY
                     TRYDIPTEGYPSLGPDDAPITIVEFSDFQCPFCRRFHSETYQALLDAYPGQIRFVYRN
                     LPLTSIHPDAMPSAIASLCANDQNVYWEYHDKLFSSETLTRDTFIQYATDLNLNVDEF
                     SACLDSGKHDENIQQDMDFALNLGVQSTPTFFVNGLAIVGAQPLSSFTQIIDMELAGE
                     IP"
BASE COUNT          451 a          497 c          342 g          436 t
ORIGIN      
        1 catgtgctaa tctttatcaa tctcgattat ggtctagcat ggagcatgat cgctcgtacc
       61 gtttttgaaa agccctatat catcgttggt gcgttggcat ttctcatgct cattcccctt
      121 ggcataactt cattcgatat ttggaaggtc cgcctcggca aaaattggaa acgccttcat
      181 caaatggtat atgtcattgc gcccttggtg gtgctgcatt acctgtggag caaaaaaggc
      241 gacctactta atttctcagg cgacatcgtg cgccccacga tctatggctt ggtcgttgtg
      301 gtcttcctga ttttgcgcat tcccgccatt cgacgtgcca tcgcctctct cccgaaccgc
      361 ggcttgactc tccccttcaa aaagaatccc tctcccaaag cggatgcttc ttaatttaaa
      421 taagggcgac ctgacaggtc gcccttacgc tttaaaacat cccgcgaaag aacagcagta
      481 aaccgattat tcccaaaggc acacccacaa tcgcaccgac aatcgtcagg ctgacgagaa
      541 cgccaacgac aaacaacaca atgccgagaa tcatcgccac gagccgccca gtcatctcta
      601 caatgacagc cagcaatttc cagatcgcgg caaagggcca taaataccag ggaatttgtc
      661 ttttctgagt tgttgtagtc ataagaagaa cctccatgcc taatatacgc aaaataggac
      721 aaatggttct ctaaaaagta gaatgaaaaa tacttaagta ctactgctta aatccaaaac
      781 acaggactat aatcttgcca ctatgaacac aaaatctacc cggtctaaaa aatccacgcc
      841 cgccgtgatc gaaatcgaac aagaagagcc tgtcatcgaa agcacgccag actctgaaga
      901 tttcatcacc ttcaagcgca gtcattttta ctctgtgcta gtcgtccttg cctttgctgt
      961 tggtctctta cttggcttcg tcatttgggg acgcggcagt aacaccgtta ccgtcgctgc
     1021 agacccagcc gctgctgcca ataatgctgc agcccaacaa ccttccggcg aaatcgccga
     1081 agcgcctgtt accccgcaac cacaatacac ccgctacgat atccccaccg agggctaccc
     1141 cagcctgggt cctgatgatg cgccgatcac catcgtagaa ttcagtgact tccagtgtcc
     1201 tttctgccgc cgcttccatt cggaaacata tcaggcattg ttggatgctt atcccggtca
     1261 gatccgcttc gtatatcgca atctacctct cacctccatc catcccgatg cgatgccctc
     1321 cgcgattgct tccctctgcg caaatgacca gaatgtgtat tgggaatatc acgataaact
     1381 cttcagcagc gaaactctaa cgcgcgatac ctttattcaa tatgccaccg atttgaacct
     1441 aaacgtagac gagttctctg cctgtctcga tagcggcaag cacgatgaaa acatccagca
     1501 ggatatggac ttcgctctca atctcggcgt ccaatctacc cccaccttct ttgttaacgg
     1561 tttagcaatt gtgggcgcgc agccgctttc ttccttcacc caaattatcg atatggaact
     1621 tgcgggtgaa atcccctaag ctttagaaat acaatctcaa aaggcggacg gtatcgtccg
     1681 ccttttttct taaaactccg tgccaaaatt tatcccagtg gtaaga
//
LOCUS       DAOQJE010000831         1720 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_61887_length_1720_cov_2.913514, whole genome shotgun sequence.
ACCESSION   DAOQJE010000831 DAOQJE010000000
VERSION     DAOQJE010000831.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1720)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1720)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1720
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_61887_length_1720_cov_2.913514"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..272
                     /locus_tag="PLT08_17030"
     CDS             <1..272
                     /locus_tag="PLT08_17030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE06233.1"
                     /translation="GRLRSQLNGNLAFISGEQVVATETRSIQGNQDVSIPVDVSSTTD
                     LQPQVIPERPAWILPTIVSTIVLIVIVLIVIGAQTFMQKRSDKQE"
     gene            269..>1720
                     /locus_tag="PLT08_17035"
     CDS             269..>1720
                     /locus_tag="PLT08_17035"
                     /inference="COORDINATES: protein motif:HMM:NF024832.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide biosynthesis protein"
                     /protein_id="HNE06234.1"
                     /translation="MTHLSEDGDTSEKISVRHVGRGVMWSYLSFVLGKVMVFVSTVIL
                     ARLLTPADFGLVGLASVLAGYLGTLHTFGVGEAFIQKKFGSEESANATFVLSVASGIL
                     LFLLSIIITPLVVIFFREPQIQSIFPVLAFTYVLTGLTTIHDALLVKELKFQQRLLPT
                     FVQYMSKGIASIVLALMGFGAWAIVWGVLIGTLAKSITLIFVSPWRPSRAWDSKIARE
                     IFGFGKFMVLQNIFGALEDNIDYMIVGRRLGVTDLGLYTLGYRAPEMAIISLPGVISN
                     VAYPAYAKLQDDFGELQRSVRKTVQIISWLAIPAGLGLAMISSSFILTFYTKKWESTI
                     PVMQLLSLYAMVYTLSYNFGDAYKAMGRPDILNNISISTILFTIFALWVGARYGIIGV
                     AWAHLIRVLVLLGIQIVIVKRVLSIPYGDILKSIFNPLVCSIIMVSVMAFVSKEFQTL
                     STPLLLVLQLFVGGSTYLVSSFLINKDTTIFILN"
BASE COUNT          440 a          279 c          371 g          630 t
ORIGIN      
        1 ctggaagatt gcgcagtcaa ctaaacggaa atcttgcctt tataagtggg gagcaggttg
       61 ttgctacaga aacgagatct attcaaggta atcaggatgt ttctataccg gttgatgttt
      121 cctcaactac agatttacag ccgcaagtta ttccagaacg cccagcttgg attttgccta
      181 caattgtttc tacaatcgta ttaatagtga ttgttcttat tgttattgga gcacaaacat
      241 ttatgcaaaa gcgatcggat aagcaagaat gactcatctt tcagaagatg gtgatacatc
      301 agaaaaaata tcggtgcgac atgttggacg tggggttatg tggtcctact tgtcttttgt
      361 tcttggtaag gtgatggttt ttgtaagtac agtaattctt gcgcgccttc taacaccagc
      421 agactttggt ttggtagggc ttgcatctgt attggcaggc tatctaggaa cacttcacac
      481 ctttggcgtg ggagaggcgt tcattcaaaa aaaatttggt tccgaggaat ctgcaaatgc
      541 tacatttgta ttatcggttg catctggtat cttgcttttc ttactatcca ttattattac
      601 tcctcttgtt gtgattttct ttcgagaacc tcaaatacaa agtatttttc cagttctggc
      661 atttacatat gtgttaactg gattgactac tattcatgat gctttactag ttaaagaatt
      721 gaaatttcag cagcgtttat tgccgacatt tgtgcagtac atgagtaaag gaattgcttc
      781 tattgtttta gctttgatgg gatttggtgc atgggctatt gtttggggtg ttcttattgg
      841 tacattggca aaatcaatta ccttgatttt tgtcagccca tggcgcccat ctcgtgcttg
      901 ggactcaaaa attgcacgtg aaatttttgg ttttgggaaa tttatggttc ttcaaaatat
      961 ctttggtgcc ttagaagata atattgatta tatgattgtt ggtcgtcggc ttggtgtaac
     1021 ggatttaggg ttatacactc ttggttatcg tgctcccgaa atggcgatta ttagtttacc
     1081 aggcgtcatc tctaatgttg cctaccctgc gtatgcgaaa cttcaagatg attttggaga
     1141 gctgcaacgt agtgttcgta aaacagttca gatcatttct tggcttgcga ttcctgctgg
     1201 attaggactg gcaatgatta gctcttcgtt tattctcact ttttatacta agaaatggga
     1261 atcaaccatc cctgttatgc aattactgtc gctatatgca atggtttata ctttatctta
     1321 taattttgga gatgcgtata aagcaatggg aaggcctgat atcttaaata atattagtat
     1381 ttctacaatt ttatttacga tttttgcttt gtgggtgggg gcgcgatacg ggattattgg
     1441 agtcgcttgg gctcatttga ttagagtcct tgtgctgtta ggaattcaaa tagttattgt
     1501 taagagagtc cttagtatac cttatggtga tatattaaaa agtatattta accctcttgt
     1561 ctgctctata attatggtat cagtaatggc ttttgtgtct aaagaatttc agacattgag
     1621 tactccgcta ttgttggttt tgcaattatt tgtaggcgga agtacttatt tagtgtcatc
     1681 ttttttaatc aataaagata cgacgatttt tatcttgaat
//
LOCUS       DAOQJE010000832         1720 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_61912_length_1720_cov_2.283483, whole genome shotgun sequence.
ACCESSION   DAOQJE010000832 DAOQJE010000000
VERSION     DAOQJE010000832.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1720)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1720)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1720
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_61912_length_1720_cov_2.283483"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..552)
                     /locus_tag="PLT08_17040"
     CDS             complement(<1..552)
                     /locus_tag="PLT08_17040"
                     /inference="COORDINATES: protein motif:HMM:NF012336.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE06235.1"
                     /translation="MQVNIPLKIIGLGRYLPKRVVPSSELEAMCGVPAGWVERRNGVR
                     ERRWVTDETSSFMSAEAGREALNEAKLKPQQLDLIINASGTGEQAIPDTGVLIQRQLG
                     LGNSGIPAMTVHTTCLSFVAAMDVASNFISSGRYKNILICSADVASCGINPKEPESAS
                     LVGDAAAAVVVTRAEEGEASRIHY"
     gene            880..>1720
                     /locus_tag="PLT08_17045"
     CDS             880..>1720
                     /locus_tag="PLT08_17045"
                     /inference="COORDINATES: protein motif:HMM:NF016090.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="circularly permuted type 2 ATP-grasp protein"
                     /protein_id="HNE06236.1"
                     /translation="MPDSVLSNLYDPAPFYDEMFSSAGQPRPHYEVLRNYLHGMTADM
                     FAERRRAADAAFLYQGITFTVYGQDDGIERIFPFDLVPRIIPHNEWDQIERGLIQRVT
                     ALNLFLHDIYHEQRILREKRIPSELVFSSRHFRHEMIGVNVPKDRYVHIVGTDIVRGG
                     DGEYYVLEDNLRSPSGVSYMLENRQAMKQTFADLFSRYGVLPIEHYPQELLNTLRSVA
                     PHDYSDATVVLLTPGIYNSAYFEHTFLARQMGIEIVEGRDLVAHQNKIYMRTTRGLKL
                     VDVI"
BASE COUNT          397 a          506 c          389 g          428 t
ORIGIN      
        1 ataatggatt ctcgatgctt caccttcttc tgcccgagtc accaccactg ccgccgccgc
       61 atcgcccact aacgaagcgg actccggttc ctttgggttg atgccacacg aagctacatc
      121 ggcgctgcag atcaaaatat ttttatagcg tccgctactg atgaagttcg aagccacatc
      181 catcgccgcc acgaagctca agcaggtcgt atgcaccgtc atcgccggaa tgcccgagtt
      241 gcccagccca agctgtcgct gaatcaaaac ccccgtatct ggaatcgcct gttcccccgt
      301 gcctgaggcg ttgatgatca aatccaattg ctgtggcttg agtttggctt cgttcaacgc
      361 ctcccttccc gcctccgccg acatgaacga tgacgtttca tccgtcaccc aacgacgctc
      421 acgtacccca ttgcgtcgct ccacccaacc ggcgggcacg ccacacatcg cttccaattc
      481 agaactgggc acaactcgtt tcggtaaata acgtcctaaa ccaataattt ttaggggaat
      541 gttcacttgc atagaaaggg ctcccaatca ggttgatttt attaggactt acgcaaaatc
      601 atttgtttct cgagcatttg accgccgacg gcagaccctt cgacaagctc aggacatcgc
      661 cgcggtcagt ggtcggcggt caatttttag gagttttgcg taagtcttgt ttataagatt
      721 atctcatacc cgtaaaaaat cggcataaac ttttttctct gtgcgctctg tggctaacct
      781 tcccacaagg gtcacaagaa tactacccat aagggtacct tcagcttttt gcctgcctca
      841 agtatttcat ggtaaaaata gccaaagagc caatactcaa tgccggattc cgttctctcg
      901 aacttgtatg atcccgctcc attctatgat gagatgttct catctgccgg gcaaccacgc
      961 ccgcactatg aagtgctgcg aaactatcta catgggatga cggcagacat gtttgccgaa
     1021 cgccgccgcg ctgccgatgc ggcgttttta tatcagggca tcaccttcac cgtgtatggt
     1081 caggatgatg gcatcgaacg catcttcccc ttcgacctgg tgccgcgcat cattccgcac
     1141 aacgagtggg accagatcga gcgcggactc atccaacggg tgacggcgct caacctcttc
     1201 ctccacgata tttatcacga acaacgcatc ctgcgcgaga agcgcattcc atctgaattg
     1261 gtctttagct cccgccattt ccgccatgag atgatcgggg tcaatgttcc caaagatcgc
     1321 tatgttcaca tcgtcggcac agatatcgtg cgcggcggcg atggggaata ttacgtgctt
     1381 gaagataatc tgcgttcgcc ttctggcgtg agctatatgc ttgagaaccg tcaagccatg
     1441 aagcaaacct tcgccgacct gttttcacgc tatggcgttt tgcccattga acattatcca
     1501 caagaattac tgaacacgct tcggtctgtc gccccgcatg attattcaga tgcgaccgtg
     1561 gtcttgctca cgcctggcat ttacaactcc gcttactttg agcatacctt tctggcacgc
     1621 caaatgggca tcgagatcgt ggaaggacgc gacctggtgg cgcatcaaaa caagatctac
     1681 atgcgcacca cccgcggact caaactggtc gatgtcatct
//
LOCUS       DAOQJE010000833         1717 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_62096_length_1717_cov_3.361613, whole genome shotgun sequence.
ACCESSION   DAOQJE010000833 DAOQJE010000000
VERSION     DAOQJE010000833.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1717)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1717)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1717
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_62096_length_1717_cov_3.361613"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..251
                     /locus_tag="PLT08_17050"
     CDS             <1..251
                     /locus_tag="PLT08_17050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE06237.1"
                     /translation="RQTLPMWEDVKSETVLCVSGPLKQIYTRVSTGRYTSINLVHYRI
                     AKKFFDVAFFATKLIPQNQRCNVYYTPKSEIIIGIEPI"
     gene            420..1151
                     /locus_tag="PLT08_17055"
     CDS             420..1151
                     /locus_tag="PLT08_17055"
                     /inference="COORDINATES: protein motif:HMM:NF015438.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MOSC domain-containing protein"
                     /protein_id="HNE06238.1"
                     /translation="MITLIGHIYNLVRYPVKSMAGISIDSAFLGWHGLQGDRRFAFRR
                     INNKSNFPWLSASRLPELLLYEPFGEDDKEEEPLPTHVRTPEGTILPLGSIELQSSIA
                     EKFGDSVELMKLKHGIFDEASVSVINLTTISAIGKEIEQSLDPRRFRANIIIEADSSE
                     PFMENNWIGKTLLFGSKEDGVMLNITLKDLRCMMINLDPNTAEQDPRIMKTVVHLNEN
                     NAGAYGTVVRTGNLSVGLPVSLIGD"
     gene            1327..>1717
                     /locus_tag="PLT08_17060"
     CDS             1327..>1717
                     /locus_tag="PLT08_17060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE06239.1"
                     /translation="MKTRFALQYLLCTIFLAACGGVTPIPIVSTASPTIAISATKQLP
                     TPVHIPTLTPTLWNTPTPETLFGYPTSLPAVLPTCNPIDCFKMEDNVQRQDFTFHDLY
                     LGKYVLRNWCNNDPHFTIYSYCAVTISS"
BASE COUNT          501 a          381 c          354 g          481 t
ORIGIN      
        1 tgcgtcagac gctgccaatg tgggaagatg ttaaatcaga gaccgttcta tgtgtttcag
       61 gaccactaaa gcaaatatat actcgagtca gtactggacg ctatacttca ataaatctag
      121 ttcactatag aatagcaaag aagttttttg acgtcgcttt ttttgcaact aaacttattc
      181 cgcaaaatca aagatgcaat gtgtactaca caccgaagag cgagatcatt attggaattg
      241 aaccaattta aagttgccca acaaagcgtg cacctgacgc tggggactct gcggcaatct
      301 caagcagttt cctacgcctt atcatttttc tggctggacg gcttcgccaa gtccgcgcac
      361 cggggcaggt aacgcaaacc gttgggcagt tccttgtata gcataggcat acagtaatta
      421 tgattactct tatcggacat atttataatc ttgtcagata cccagtcaaa tctatggcag
      481 gtatctcaat agactctgct tttcttggtt ggcacggttt gcagggagac cgacgttttg
      541 catttcgtcg aatcaataat aaaagcaatt ttccttggct ttcagccagc cgcctgcctg
      601 aacttctttt atatgaaccg tttggggaag atgacaagga agaagaacca ttgcctacac
      661 atgttcgtac accagaaggc acaattctgc cattaggaag cattgagttg caaagcagta
      721 tcgccgaaaa gtttggcgac tccgtcgaat tgatgaaact caagcatggt atttttgatg
      781 aagctagtgt gtcagtgatt aatttaacaa caatttctgc tattggcaaa gaaatagaac
      841 aaagcctaga ccctagacga tttcgggcaa atatcattat tgaggcagat tcatcagaac
      901 catttatgga aaataattgg atagggaaaa cattgctttt tggcagcaaa gaagatgggg
      961 taatgctcaa tattacatta aaagacttac ggtgtatgat gattaatctc gacccgaata
     1021 ccgccgaaca agaccctcgt ataatgaaaa ccgtcgtaca tcttaatgag aataatgcag
     1081 gtgcttatgg aacagttgtg agaacgggca atcttagtgt aggtctgcca gtcagtctta
     1141 taggtgatta atcaggtatg tacttaaatt gacaaactgc ccaacaaagc gtgcacctga
     1201 cgctggggat tctgcggcaa tctcaggcag tttcctacgc cttagcattt ttccagttgg
     1261 acggcttcgc cgtccccacc ccagcgcagg taacgcaatc cgttgggcgc ttcgttgcaa
     1321 aaattcatga aaactagatt tgcgttgcag tatcttttat gtacgatttt tcttgccgct
     1381 tgcggtggag ttacccccat cccaatagta tcgacggcat caccaacaat tgccatttct
     1441 gcaaccaagc aacttccgac cccagttcat atacctacgc taacgccaac gctctggaat
     1501 actccgacgc cagaaacact ctttggttat ccaacttctt tgcctgcggt gttgcccaca
     1561 tgcaatccaa tagattgttt caaaatggaa gataacgtcc aaaggcaaga ctttactttt
     1621 catgatttat atttaggcaa atatgtctta agaaattggt gtaacaatga cccccacttt
     1681 actatatact catattgtgc cgtcacaatt tcatcaa
//
LOCUS       DAOQJE010000834         1716 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_62154_length_1716_cov_5.387718, whole genome shotgun sequence.
ACCESSION   DAOQJE010000834 DAOQJE010000000
VERSION     DAOQJE010000834.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1716)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1716)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1716
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_62154_length_1716_cov_5.387718"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..196
                     /locus_tag="PLT08_17065"
     CDS             <1..196
                     /locus_tag="PLT08_17065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE06240.1"
                     /translation="SSVLVKTRSRGAGLSAVLLRALFAKFPNKVWHVSPIYPEEMGVI
                     FEHVGMQRETLSQWQMSRVL"
     gene            206..868
                     /locus_tag="PLT08_17070"
     CDS             206..868
                     /locus_tag="PLT08_17070"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acylneuraminate cytidylyltransferase family
                     protein"
                     /protein_id="HNE06241.1"
                     /translation="MKLAALVPMRHHSQRVPGKNYRPLAGKPLFQHILETLQAVPEID
                     TVIVDTDSEPVMDGVRRLFPNVKLIQRPEHLRADNVPMNDILLYDTAQVQVDFYLQTH
                     STNPLLKTETISRGIKSFLADYPKYDSLFSVTRLQTRLYWQDGRAINHNPLELLQTQD
                     LPPVYEENSCVYLFTRENLERKKHRIGDKPFMFEIDADEAWDIDEELDFEIADFLMRK
                     RQ"
     gene            872..>1716
                     /locus_tag="PLT08_17075"
     CDS             872..>1716
                     /locus_tag="PLT08_17075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE06242.1"
                     /translation="MQSLKQKLGTFGWGSLFLTLLGAVTYLPFAARMGYINDDWYLMF
                     DGYVGGASFFHEVFIVDRPLRGYVMQAAFSLFGMNPLYYHLSAFLFRVVSAVALFWIG
                     NHLWQRRTTYNWLAALLFLVYPGFLSQVNPIDYQSQIFSLACGMVSVALTLKALQAKT
                     SVARWIYISASIVLTWVYLGLVEYFLGFETLRFAVIGILYLRQTEQPVFIRLKSAVVT
                     SLPFLAGVGGFLVWRLFFFEAERKATDVSLQLGSAFTSPLTVLWWLNYLVQDVFKVIM
                     VAWTVP"
BASE COUNT          382 a          427 c          410 g          497 t
ORIGIN      
        1 ttcgtcggtg ttggtcaaaa cccgctcacg tggagcaggc ttgagcgcgg ttctgttgcg
       61 cgcacttttt gcgaagttcc caaataaagt ttggcacgtc tcgccaatct atcctgaaga
      121 aatgggcgtt atcttcgagc atgtgggcat gcaacgcgag acgctttcac aatggcaaat
      181 gtcacgcgtg ttgtaaaata agggcatgaa attagccgcc cttgttccca tgcgtcatca
      241 tagccagcgt gtgcctggca aaaattatcg cccgctcgca ggcaaacccc tctttcaaca
      301 tattttagaa accctgcaag ccgtgccaga gattgacacg gttattgtgg ataccgactc
      361 agagccagtg atggatggag tgcgccgcct ctttcctaac gtaaaactga ttcaacgccc
      421 cgagcatctc cgtgctgaca atgttcccat gaacgatatt ctcctgtacg atacagcgca
      481 agtacaggta gatttttact tgcaaaccca ttccaccaat ccgctgctca aaaccgaaac
      541 catctcccgt ggaatcaagt ccttccttgc tgattaccct aaatatgact cgctcttctc
      601 ggtgacgcgc ttacaaaccc gtttatattg gcaggatggg cgcgctatca atcacaatcc
      661 actggagcta ttgcaaaccc aagaccttcc gcccgtgtat gaagagaatt cctgcgtcta
      721 tttgttcact cgtgagaatc tggaacgcaa aaaacatcgc attggcgata aaccttttat
      781 gttcgagatc gacgccgacg aagcctggga cattgacgaa gaattggatt ttgagatcgc
      841 agactttttg atgaggaaga gacagtaatt catgcaaagc ctgaaacaaa aacttggcac
      901 attcggttgg ggcagccttt ttctgactct cctcggtgca gtgacttatc ttcccttcgc
      961 tgcacgcatg gggtatatca acgatgactg gtatctcatg ttcgacggtt atgtgggtgg
     1021 agcctctttt ttccatgaag tcttcatcgt agaccgcccc ctgcgcggat atgtaatgca
     1081 ggcggcgttc tcgctctttg gcatgaatcc gctgtattac cacttgagtg cattcctgtt
     1141 ccgtgtggtt agtgctgtgg ctttgttttg gatcgggaac cacctttggc aacgtcgcac
     1201 cacctacaac tggttggcgg cattgctgtt cttggtctat ccgggttttc tttcgcaggt
     1261 aaaccccatc gactatcaat cgcagatttt tagccttgcc tgtggcatgg tctctgtggc
     1321 gttgaccttg aaagccttgc aagcgaagac atctgtggca cgatggatat acatcagtgc
     1381 ttctattgtg ctgacctggg tttaccttgg cttggtggaa tacttccttg gctttgaaac
     1441 attgcgtttt gctgtcatcg gaattttgta tcttcgccaa actgagcaac ctgtttttat
     1501 ccgtttgaaa tctgcagttg taacctctct acccttccta gcaggtgtgg gcggatttct
     1561 agtttggcgt ttgttcttct ttgaagcaga acgcaaagcg accgatgtca gcttgcaatt
     1621 gggctctgcc tttacatctc cgctgacagt cttatggtgg ttgaattatc ttgttcaaga
     1681 tgtgttcaag gtcatcatgg tggcgtggac agtgcc
//
LOCUS       DAOQJE010000835         1716 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_62163_length_1716_cov_3.957857, whole genome shotgun sequence.
ACCESSION   DAOQJE010000835 DAOQJE010000000
VERSION     DAOQJE010000835.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1716)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1716)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1716
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_62163_length_1716_cov_3.957857"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1509
                     /locus_tag="PLT08_17080"
     CDS             <1..1509
                     /locus_tag="PLT08_17080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008705 - methionine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 dependent-methionine synthase
                     activation domain-containing protein"
                     /protein_id="HNE06243.1"
                     /translation="INREPTGERSARHEVAVHVLNNVLLPAMKEVGDKFGAGELILPF
                     VLQSAEVMKKTVSHLENYLEKVEGVSKGLVVLATVYGDVHDIGKNLVKTILANNGYDV
                     IDLGKQVPAETIITKAVEVNATAIGLSALLVSTSKQMPLIVNELQRRKLNFPVLIGGA
                     AINRRFGRRILQTEDGATYEAGVFYCKDAFEGLETMDTLIDKNKRPELLAKIRKESEM
                     ELNRASQTEQSAVSGQRSKVVASPIALPAKLGQRIVKDMPLEMVLKHLNMNELYRLSW
                     GAKNTHGAEWEKLKAEFDARLAKMTKDVLKEGWLKPQAVYGYFACQADGNDLIIYDDA
                     ESKKEISRFTFPRQPYDDHLALSDYYASVESGQIDVVALQVVTVGQAASDKFDKLQAA
                     NDYTEAYFVHGLAVQAAEATANYLHEHVRRELGIGESQGKRYSWGYPAIPELEDHFKV
                     FKLLPAAESELGMSLSISGQLIPEQSTAAIIVHHKDAKYYAVGESRVEQLTK"
BASE COUNT          425 a          478 c          428 g          385 t
ORIGIN      
        1 atcaaccgcg aaccgaccgg tgaacgctcc gctcgccatg aagtggctgt gcatgtgctc
       61 aataacgtcc tccttcccgc catgaaagaa gtgggtgaca agtttggtgc aggcgagttg
      121 attctgccct ttgtgcttca gtctgccgaa gttatgaaga aaaccgtctc gcaccttgag
      181 aattatcttg aaaaggtcga aggcgtcagt aagggactgg tcgtgctcgc caccgtctac
      241 ggcgatgtgc atgacatcgg caaaaattta gtaaaaacca ttctcgcgaa taatggctat
      301 gacgtgatcg acctcggtaa gcaagtccct gccgagacga ttatcactaa agccgtcgaa
      361 gtcaacgcga ctgcgatcgg tttgtcggcg ttgctcgtgt ccacatccaa gcaaatgccg
      421 ctgatcgtga acgaactcca acgtcgcaag ctcaacttcc ccgtcctgat cggaggcgct
      481 gccattaatc gccgctttgg tcgtcgcatc ctgcaaactg aagatggcgc aacatatgaa
      541 gcgggcgtct tttattgtaa ggatgccttc gaaggtcttg aaacaatgga tacgctcatt
      601 gacaagaaca agcgccctga gctgctcgcc aagatccgca aagaatctga aatggaactc
      661 aaccgcgctt cacaaactga gcagtcagcg gttagcggtc agcgctcgaa agttgtggca
      721 tcgccgatcg ccttgcccgc caaactcggt cagcgcattg tcaaagacat gccgctcgaa
      781 atggtcttga agcacctcaa catgaacgag ttgtatcgtc tctcgtgggg cgcgaagaac
      841 acccatggcg cagaatggga aaagctcaaa gccgagttcg atgcccgtct cgctaagatg
      901 accaaggatg tgctcaaaga aggctggctc aagccgcaag ctgtgtatgg ctacttcgcc
      961 tgtcaggctg atggcaatga ccttatcatc tacgatgatg ctgaaagcaa gaaagaaatt
     1021 tcgcgcttca ctttcccgcg ccaaccctac gatgaccatc tcgcgctctc cgattactac
     1081 gcctccgtcg aatccggtca aatagatgtg gtcgcgcttc aggtcgtgac tgtcggtcaa
     1141 gccgccagcg acaaattcga caagctgcaa gccgccaacg actacaccga agcctatttc
     1201 gtgcatggtc tcgccgtcca agccgccgaa gcgactgcca actatctcca cgaacatgtc
     1261 cgccgcgagt tgggcattgg cgaaagccag ggcaagcgtt actcgtgggg ctaccccgcc
     1321 atccctgaac tcgaagatca cttcaaggtc ttcaaactgc tgcccgcggc tgagtctgag
     1381 cttggcatga gcctctccat ttctggtcag ctaatccctg aacaatccac tgccgcgatt
     1441 attgtccatc acaaggatgc gaagtattac gcagtgggtg agagccgcgt ggagcaattg
     1501 accaagtaag agctttccgc acaaagacag agagacgctg tgctccctgt cttttaagtt
     1561 acacttgcac cacgatgaag aaatcactgc aaactacatt caatatttgg attgccctta
     1621 ctctattggt actggttagc cttgtcacca tctatcttcg taacggcatg gacggaatca
     1681 acctgttcat tcaggagtgg ccgctttcca acctgg
//
LOCUS       DAOQJE010000836         1715 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_62230_length_1715_cov_4.496386, whole genome shotgun sequence.
ACCESSION   DAOQJE010000836 DAOQJE010000000
VERSION     DAOQJE010000836.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1715)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1715)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1715
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_62230_length_1715_cov_4.496386"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..385)
                     /locus_tag="PLT08_17085"
     CDS             complement(<1..385)
                     /locus_tag="PLT08_17085"
                     /inference="COORDINATES: protein motif:HMM:NF015331.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HNE06244.1"
                     /translation="MPNRIATASIDIAAPIHVVWAAMLDFDAYSEWNPFIYKVSDLPE
                     TLTLGSRFLLHVRWANGSSYRSWETLTQLEPPAPSRDGVHTALLTYRYASWQVRLGLV
                     RAERRQVLSRPAVGVTHYATQESFHG"
     gene            complement(428..1441)
                     /locus_tag="PLT08_17090"
     CDS             complement(428..1441)
                     /locus_tag="PLT08_17090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HNE06245.1"
                     /translation="MPEYKSLDGNTPEGKRTAQNLIRLRFELDNAIPARTVDSTLLLA
                     TWNIREFGGTKSDGREAEPLYYIAEIISHFDMVAVQEVRDNLDALDELMKILGPWWQY
                     LVSDVTQGAQGNDERHAFIYDTRKIAFGGLSGELVPEMKKNKDGTLASDFAFARTPYI
                     AGFKAGWFKFTLCTQHFYYGDEKPDDPQRLKESQNVVKLLKKRMQSKDSWANNSILLG
                     DFNIFSVKDETFQAIEKESFQIPAKLKGTYSNANRDKPFDQIAFLAKDVEKQLGLAKA
                     GTFPFFDFVYRDDDLNTYQPGSTLAKYKQWRTFKMSDHLPLWVELYVDFGEAYLQKKM
                     GGG"
BASE COUNT          379 a          436 c          473 g          427 t
ORIGIN      
        1 acccatgaaa ggactcttgg gtggcgtaat gcgtgactcc gacggcgggc cgggacaaaa
       61 cctgccgccg ttcggcgcgc accaagccca aacggacctg ccaagaggcg taacgataag
      121 tcagcagcgc ggtgtggacg ccatccctgc ttggggcagg gggctccagt tgggtgagag
      181 tctcccatga gcggtagcta gagccatttg cccagcgcac atgcaaaagg aagcgtgagc
      241 cgagcgtcag tgtttcaggc aggtcgctga ccttatagat aaagggattc cattcagagt
      301 aggcatcaaa atccaacatc gccgcccaca ccacatggat gggggcagcg atgtctatag
      361 aagcagtcgc aattcgattt ggcatgccct tattataggg ctccgtttgt gatgaagcaa
      421 tcgctcttta cccgccaccc attttctttt gtaaatatgc ctcgccgaag tcgacataga
      481 gctcgaccca caagggcaga tggtcggaca ttttaaaggt gcgccattgc ttgtacttcg
      541 ccagcgtgct gccgggttga taggtattca ggtcgtcgtc acggtagaca aaatcgaaga
      601 aggggaaggt gcccgccttg gcgagaccga gttgtttctc cacgtctttg gcgaggaagg
      661 cgatctgatc gaagggctta tcgcggttgg cgttgctata ggtgcccttg agcttggcag
      721 ggatctgaaa ggactctttc tcgatggctt ggaaggtttc atctttgacc gagaagatgt
      781 tgaaatcacc caacagaatg ctgttgtttg cccaactgtc tttcgattgc atgcgtttct
      841 tgagcagctt caccacgttc tgtgactctt tcaggcgctg cgggtcatcg ggcttttcgt
      901 cgccataata aaagtgttgg gtgcagagcg tgaacttgaa ccagccggct ttgaagcccg
      961 cgatataggg cgtgcgcgca aaggcaaagt ctgaggcgag cgtgccgtct ttgttcttct
     1021 tcatctcggg caccagttcg cccgaaagcc caccgaaggc gatcttgcgc gtatcgtaga
     1081 tgaaggcatg gcgttcgtcg ttgccctgcg ccccctgggt gacgtcggac accagatact
     1141 gccaccacgg accgaggatc ttcatcagct cgtccagcgc atccaaatta tcgcggacct
     1201 cctgcacggc gaccatgtcg aaatgcgaaa tgatctcggc gatgtaatac agcggctcgg
     1261 cttctctgcc atcggacttg gtcccgccaa actcgcggat gttccaggtg gcgagcagca
     1321 aggtcgagtc gaccgtgcgg gcagggatgg cgttatccaa ctcgaagcgc aagcggatca
     1381 ggttctgggc ggtgcgtttc ccttcggggg tgtttccatc gaggctttta tattcaggca
     1441 tgtcggcttt cctctctttc aacaaagaat atacggctta tttcagtata gccatttcac
     1501 agccccattt caatgcatgg acttgtaagg tgtgactaca atttgtatat aacctgtcct
     1561 gcgtattggc aaagggatat tatgaatcta agcggattca cttagtgccc gattgagaag
     1621 atcagcgatt tttagatcaa tagcccactt ttttagatat cctagccttt caagaacatt
     1681 tgttaccttt agtgtaactt caataggttc attct
//
LOCUS       DAOQJE010000837         1712 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_62438_length_1712_cov_6.978877, whole genome shotgun sequence.
ACCESSION   DAOQJE010000837 DAOQJE010000000
VERSION     DAOQJE010000837.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1712)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1712)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1712
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_62438_length_1712_cov_6.978877"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            18..671
                     /locus_tag="PLT08_17095"
     CDS             18..671
                     /locus_tag="PLT08_17095"
                     /inference="COORDINATES: protein motif:HMM:NF023580.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3592 domain-containing protein"
                     /protein_id="HNE06246.1"
                     /translation="MAKKKYSINWEDDLPVSFEVNGVQYQNIEDVPDEKDRFKLEAML
                     NSSFDAEFNDPQLEKMMKETKQMDGASAEAWIVRIFTSVAVVMLLVAGISTVFVLQKL
                     GREESVPGVVVDVVEQRYEDAETDNVSYYYYPVVEFKARDGKRRTVQLTEGSNPQSNE
                     VGDEVTILYEPENPNDARIKSFGSSALMWILPSITGILGAGFLGAVLVFQKYLANVL"
     gene            complement(703..1281)
                     /locus_tag="PLT08_17100"
     CDS             complement(703..1281)
                     /locus_tag="PLT08_17100"
                     /EC_number="2.1.1.100"
                     /inference="COORDINATES: protein motif:HMM:NF040696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004671 - protein C-terminal
                     S-isoprenylcysteine carboxyl O-methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-S-isoprenylcysteine O-methyltransferase"
                     /protein_id="HNE06247.1"
                     /translation="MSIFEIVYWAALVVEMVIRAPLNKKRKEEKISEQRVSQQEKLIL
                     FLLLIGMMILPLIYSATSWLDFANYNLPPWAGYLGALIMLSALFVFWRSHADLGLNWS
                     PSLEIRDKHELITRGIYGVIRHPMYASQWLWVLAQPLLLQNWLAGFINLLVFIPFYLL
                     RVQAEEKMMIDRFGEQYKTYMTKTGRVVPKLG"
     gene            complement(1278..1532)
                     /locus_tag="PLT08_17105"
     CDS             complement(1278..1532)
                     /locus_tag="PLT08_17105"
                     /inference="COORDINATES: protein motif:HMM:NF025601.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4342 domain-containing protein"
                     /protein_id="HNE06248.1"
                     /translation="MSERTYTEEFRVEGEKIIAKIKELLHEGNIRKVIIKDKDGKVLM
                     EIPVTIGVVGVLIAPQLAALGAIAALLTEATVIVEKTETL"
BASE COUNT          444 a          360 c          419 g          489 t
ORIGIN      
        1 tgcgctcgga ggtatgaatg gcaaagaaaa aatattccat caattgggaa gatgatcttc
       61 ctgtttcgtt tgaagtgaat ggtgtgcaat atcaaaacat tgaagatgtg cccgatgaaa
      121 aagaccgctt caagttggaa gcaatgttga attcttcttt tgatgcagag ttcaatgacc
      181 ctcaacttga aaagatgatg aaggaaacca agcagatgga tggtgcctct gctgaagcgt
      241 ggatcgtgcg catctttaca agtgttgctg ttgtgatgtt gttagtggct gggatttcta
      301 ctgtatttgt cttgcaaaaa ctggggcgtg aagagagtgt gcccggtgtt gttgtggatg
      361 tggttgagca gcgttatgaa gatgctgaga cggacaacgt cagctactac tattatccgg
      421 tagtggagtt caaggcacga gatggaaagc gccgcacagt tcaattgacg gagggtagta
      481 acccgcaatc gaatgaggtg ggggatgagg tgacgatttt gtatgagcca gaaaacccga
      541 atgacgcccg cattaagtct tttggtagct ctgccttgat gtggattttg ccaagcatta
      601 cgggtatttt aggcgctggg ttcttgggtg cggtacttgt atttcagaag tatttagcaa
      661 acgttttgtg agaaaaatcc cgccatctgg cgggattttt ctctaaccaa gttttggcac
      721 tactctgcct gttttggtca tatacgtttt gtattgttcg ccgaagcggt ctatcatcat
      781 tttttcttct gcttgaacac gcaagagata gaagggaatg aacaccagaa ggttgataaa
      841 cccagccagc caattttgca gcagcaaagg ttgtgcaagc acccataacc attgcgaagc
      901 atacatgggg tgacggatca cgccataaat gccacgggta atgagctcat gcttatcgcg
      961 aatttccaag gatggagacc agttcaagcc aaggtctgcg tgagagcgcc agaaaacgaa
     1021 taaagcgctt agcataatca atgcacccaa ataaccagcc caaggcggca agttatagtt
     1081 tgcaaaatcc agccatgaag tagcggagta gataagaggg agaatcatca taccaatgag
     1141 aagcaaaaag agaataagct tttcctgttg cgaaacgcgc tgttcgctta ttttttcttc
     1201 tttgcgtttc ttattaagcg gggcgcgaat gaccatctcc acgaccaaag ccgcccaata
     1261 aacgatttcg aaaatactca tagggtctct gttttttcaa caattacagt tgcttctgtg
     1321 agcaaggctg caatagcgcc caatgcggca agctgtgggg cgattaacac accgacaaca
     1381 ccgatcgtga cggggatttc cattaagacc ttgccatctt tatctttgat gataactttg
     1441 cgaatatttc cctcgtgtag caattccttg atcttggcga tgattttttc gccttccaca
     1501 cgaaactctt cggtataggt tctttcactc atgcgtttat cctttcagga tttgttcctg
     1561 taatactcga accatttttc atcgagcggc tgccattcat cgctttgatc ggcacgtgct
     1621 tcccaatttt ctttgccgct ccattcttcg attttgatgg catacaccga cgtggctcgc
     1681 agttctttgt cgttgatctc gcgatattcc tg
//
LOCUS       DAOQJE010000838         1710 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_62589_length_1710_cov_5.312991, whole genome shotgun sequence.
ACCESSION   DAOQJE010000838 DAOQJE010000000
VERSION     DAOQJE010000838.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1710)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1710)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1710
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_62589_length_1710_cov_5.312991"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..193
                     /locus_tag="PLT08_17110"
     CDS             <1..193
                     /locus_tag="PLT08_17110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE06249.1"
                     /translation="TPRIADQLSAKLEAFVFCHSPDNYLAPFIAKEFHSQISSRIKIA
                     PLPWDDLETPSEPFQPTLF"
     gene            complement(202..1329)
                     /locus_tag="PLT08_17115"
     CDS             complement(202..1329)
                     /locus_tag="PLT08_17115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE06250.1"
                     /translation="MTNQIEPEPIQIKKTNKWLLAFLIFLVLCAVTCLILLIIRQRNS
                     GVIQAQQLALQGRCPEAIQILNEVIAENPKSVIAYQIRVECYLSFQSNVYTESQGYLI
                     AALEDTNIILGLKPQDASYYGKRDFILRELAPYEIYSANKYAIFDLANQDAQSAIELG
                     APSSAYVYRHYARNFIEANHCQEGLEETQKLIQQYGTQTSDYGQYYSLYLTEAYICLG
                     ELDLALESAQAITCDDPVSTCKSGLLAEIYLQSGDYENALNTVNYMINWQPAGGGWRY
                     FIRALIYYKQGQIDLAQQDLATGDAYSWYGTGVDEYVLAQLAFDNGDKVNGIYHLQTA
                     EQMFDTNYSVLRQETLKQLKKYGAEPIPQKINLPFSTPSLP"
     gene            complement(1353..>1710)
                     /locus_tag="PLT08_17120"
     CDS             complement(1353..>1710)
                     /locus_tag="PLT08_17120"
                     /inference="COORDINATES: protein motif:HMM:NF017050.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="GMC family oxidoreductase"
                     /protein_id="HNE06251.1"
                     /translation="FTLFRTGMVAEEEPGYTINAQVEGSHEVTKEFAKRINGVALGSI
                     GENLLNLPTTAHILGGAPIGKNADEGLIDENFEIHNYDGLYVIDGSVMPANPGVNPSL
                     TITALAEYAMSKINPK"
BASE COUNT          431 a          370 c          396 g          513 t
ORIGIN      
        1 gactcctaga atcgctgatc agttatcggc gaagctggaa gcgttcgtct tttgtcattc
       61 accagataat tatctcgcac cctttatagc aaaagaattt cacagccaaa tctcttcccg
      121 aatcaaaatt gccccacttc cgtgggacga ccttgaaact ccaagcgagc cgtttcagcc
      181 gacgttgttc tagtgaaatt tttaaggcaa agacggagtg ctaaatggta agttgatttt
      241 ttgtggaatt ggctctgcac catatttctt taactgtttg agggtttcct gtcttaagac
      301 actgtaattg gtatcgaaca tttgctcggc agtttgaaga tgatagatgc cattcacctt
      361 gtcaccatta tcaaacgcaa gttgtgccaa cacatattca tctacgccgg ttccatacca
      421 tgaataggca tctccagttg ctaggtcttg ctgggcaaga tcgatttgcc cctgtttata
      481 gtagatcaag gcgcggataa aatagcgcca accgccaccc gcaggctgcc agttgatcat
      541 gtagttaacc gtgttgagcg cattttcata atcaccagat tgcaagtaaa tctctgccaa
      601 caaaccagat ttgcatgtgg aaaccgggtc atcacaagta atggcttgag cgctttcgag
      661 tgccaaatcc aactcaccaa gacagatata agcttcagtg aggtatagac tgtagtattg
      721 accataatct gaagtttgtg ttccgtattg ttggattagc ttttgagttt cttccaaacc
      781 ttcctgacaa tgattggctt caataaagtt ccgggcataa tgacgataca cataggctga
      841 actgggcgca cctaactcaa tggcggactg tgcatcttga ttcgcaaggt caaaaattgc
      901 atatttattg gcagaatata tctcgtaagg tgcaagttcg cgcaaaatga aatctctttt
      961 cccgtaataa ctggcgtctt gcggttttag ccccagaatg atatttgtat cttccaatgc
     1021 agcaattaag tatccttgtg attccgtgta tacattggat tggaagctga gatagcactc
     1081 tacgcgtatt tggtaagcga tcacagattt tgggttttcg gctatgactt cattaaggat
     1141 ctgaatggct tcagggcatc tgccttgaag tgcgagttgt tgtgcttgaa tcacgccgct
     1201 gtttctttgg cgaatgatca aaagtataag acacgtgact gcacagagta cgagaaaaat
     1261 caagaaagcc aataaccatt tattggtttt cttgatttgt atgggttctg gttcaatttg
     1321 attagtcata tttcctccac tgttttttga gattattttg gattaatttt gctcatcgcg
     1381 tattctgcca atgccgtgat cgtcagtgag gggttcacac cggggttggc tggcatgact
     1441 gagccatcga tgacatacaa gccatcgtaa ttgtgaattt caaaattctc gtcgatcaac
     1501 ccttcatcgg catttttgcc gatgggagcg ccgccgagga tgtgggcggt ggtgggcagg
     1561 ttgagcaagt tctcgccaat ggagccgagc gccacaccat tgatgcgttt ggcaaattct
     1621 ttggttacct cgtgcgagcc ttccacttgc gcgttgatgg tgtagccggg ttcttcttct
     1681 gccaccatgc cggtgcggaa gagtgtgaaa
//
LOCUS       DAOQJE010000839         1706 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_62893_length_1706_cov_4.565112, whole genome shotgun sequence.
ACCESSION   DAOQJE010000839 DAOQJE010000000
VERSION     DAOQJE010000839.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1706)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1706)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1706
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_62893_length_1706_cov_4.565112"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..464)
                     /locus_tag="PLT08_17125"
     CDS             complement(<1..464)
                     /locus_tag="PLT08_17125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HNE06252.1"
                     /translation="MQKEIIIAGFGGQGVLFGGQVLAYAAMDNGLDVTWIPSYGPEMR
                     GGTANCTVVIADHEIGSPLVKNPPLAIALNLPSFDKYEAALAKGGTLVVNKSMVDHNQ
                     RRDDIHALFVPCNEIAEEIGDRKLVNMVAVGALLTALPEVKLEDVEKALEGH"
     gene            complement(466..1236)
                     /locus_tag="PLT08_17130"
     CDS             complement(466..1236)
                     /locus_tag="PLT08_17130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HNE06253.1"
                     /translation="MATPNNLVYERPEAFTEMPTHYCPGCTHGVAHRLVAEVLDEMNL
                     VEKTIGVAPVGCSVFAYNYFDTDFVEAPHGRAPAMATGVKRVLPDRVVFTYQGDGDLA
                     SIGMGEIVHAAARGENITVIFINNANYGMTGGQMAPTTLPGMKTSSSQSGRDVETQGY
                     PIKVSEMISTLDGVGYCVRRSLHDPKNIRLAKKAIRTAFETQVRGLGFSMVELLSTCP
                     TNWGMTPVNSLKFVEEHMIPAYPLGDYKISDAIKQIKV"
     gene            complement(1236..>1706)
                     /locus_tag="PLT08_17135"
     CDS             complement(1236..>1706)
                     /locus_tag="PLT08_17135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase C-terminal domain-containing
                     protein"
                     /protein_id="HNE06254.1"
                     /translation="NPLDEEQTNLRLLSRWKEVEANEVRYKEYFIDDAEIVIVGFGTA
                     GRVALSAVREARAQGIKVGLLRPITVSPFPYEALEQLIPQAEAFLVAEMNMGQMLDDV
                     LMTVRGRVPVEFYGRPGGVVPFHNEILDEIHRLASGPLAVTTDPRDSWLTRMTA"
BASE COUNT          396 a          458 c          429 g          423 t
ORIGIN      
        1 aaatgacctt caagcgcctt ctctacatct tcaagtttta cttcaggcag agcagtaagc
       61 aaggctccaa ccgccaccat gttgaccagt ttacggtcac cgatctcttc ggcgatttcg
      121 ttgcatggaa cgaacaacgc atggatgtcg tctcggcgtt gattatgatc caccatggat
      181 ttattgacca ccaatgtgcc gccttttgcc agtgccgctt cgtatttgtc aaatgagggg
      241 aggttgaggg caatcgccag cggtggattt ttgaccagcg gggagccgat ttcatgatct
      301 gcaatgacaa ctgtgcaatt tgccgtgcca ccgcgcattt ctggaccata cgagggaatc
      361 cacgttacat ctaaaccatt gtccatggcg gcgtaagcaa gtacttgacc gccaaacaaa
      421 acgccttgtc cgccaaagcc tgcgatgatg atttcttttt gcatgttaca cctttatctg
      481 tttgatcgca tcactgatct tgtaatcacc cagcgggtag gcggggatca tatgctcttc
      541 cacgaacttc aatgagttga ccggggtcat gccccagttt gttgggcaag tggagagcaa
      601 ttcgaccatc gagaagccga gtccgcgcac ttgagtctca aacgcggtgc ggatggcttt
      661 cttcgcgaga cgaatattct tcgggtcatg cagggagcgg cgcacacaat agccgacccc
      721 atccagcgtg gaaatcatct cagatacttt gatgggatag ccttgtgttt ccacatcacg
      781 cccgctttga gaagaggagg tcttcatgcc gggtaaagtg gtcggagcca tttgtccgcc
      841 ggtcatgcca tagttggcgt tgttaataaa gataacggtg atgttctctc cgcgtgctgc
      901 cgcatgcaca atttcgccca tgccgatgga tgccaagtca ccatcgcctt gataagtgaa
      961 taccacacgg tctggcagta cgcgcttcac accggttgcc atggcgggcg cacgtccgtg
     1021 cggggcttca acgaaatcgg tgtcaaagta gttgtacgca aagacggaac agcctaccgg
     1081 tgcaacaccg attgtcttct caaccagatt catttcatct aacacttctg ctaccagtct
     1141 atgtgccacg ccgtgcgtac agcccgggca atagtgggtg ggcatttcgg tgaaagcttc
     1201 gggtctttcg taaacaagat tatttggagt agccattatg cagtcatcct tgtcagccag
     1261 gaatctcttg ggtctgtggt aacagccaaa ggtccagaag cgaggcgatg aatttcatcc
     1321 aaaatttcat tgtggaaggg aaccacgccg ccgggtcgcc cgtaaaactc taccgggaca
     1381 cgcccgcgaa cggtcatcaa cacatcatcg agcatttgcc ccatattcat ttcagcgacg
     1441 aggaacgcct cagcctgcgg gatgagctgt tccagcgctt catacggaaa cgggctgaca
     1501 gtgatcggcc tcaaaagccc aaccttgatt ccttgtgcgc gcgcctcgcg gactgccgaa
     1561 agtgctacgc gccctgcagt tccaaaaccg acgatcacga tctcggcatc atcgatgaaa
     1621 tattctttgt agcgaacttc gtttgcttct acttccttcc aacggctgag caaacgtaaa
     1681 ttggtttgtt cttcatccaa cggatt
//
LOCUS       DAOQJE010000840         1705 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_62964_length_1705_cov_6.272727, whole genome shotgun sequence.
ACCESSION   DAOQJE010000840 DAOQJE010000000
VERSION     DAOQJE010000840.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1705)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1705)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1705
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_62964_length_1705_cov_6.272727"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..457)
                     /locus_tag="PLT08_17140"
     CDS             complement(<1..457)
                     /locus_tag="PLT08_17140"
                     /inference="COORDINATES: protein motif:HMM:NF025052.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNE06255.1"
                     /translation="MTHIFFLAGAPAVGKSSTARALAMQFQKSIHISVDNIRDMVVSG
                     LIHPSNEWSQELIEQLVLARESASQMAITYNKAGFVVAIDDFWDPNNKLQEYNLLFQE
                     KNIHKVLLFPSQLAAEERNIKRSGLGEASKYIADGIRTVYEHLEEDIPSL"
     gene            complement(1025..1219)
                     /locus_tag="PLT08_17145"
     CDS             complement(1025..1219)
                     /locus_tag="PLT08_17145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE06256.1"
                     /translation="MFEWLNNNVGLVLVALSVFGLVGGSFSSRGLTFKLETSKGKVNV
                     FLMVIGTVGILLIVADFLIN"
BASE COUNT          501 a          399 c          348 g          457 t
ORIGIN      
        1 caaggctggg aatatcttcc tctaaatgtt cgtaaactgt tcgtatccca tctgcgatgt
       61 atttgcttgc ttcgcctaac ccagatcttt ttatatttcg ttcttccgct gccagttgac
      121 tagggaacaa taagactttg tgaatatttt tctcttgaaa taaaaggtta tattcttgca
      181 atttgttgtt tggatcccaa aaatcgtcta ttgccactac aaaaccagcc ttgttgtatg
      241 tgattgccat ctgagacgcg ctttctcgtg ccaataccaa ttgctcaatg agttcttggc
      301 tccattcgtt gctcggatgt atcagccctg acactaccat atcccgtata ttatcaacgg
      361 agatatgtat gcttttttga aattgcattg ccaatgcacg tgcagttgaa cttttcccaa
      421 cggcaggggc accagccaaa aaaaatatat gagtcacagg catctcctta caacaaaatt
      481 cttattcttg ataaaaatat cttagggttg cccaacggat tgcggcttgc cctgtgggga
      541 tatacccttt tgcagggcac gggcccgccc tgtctcgcgg accgggcgaa gccgcccagc
      601 cagcctgtcc tatacgacag tccagggaaa actgtcggtt ctatcgggac gctttgttag
      661 gtgcatttta tgaacacaag actcattggc aattaaagaa cctgaccgca aacttgactg
      721 aattttatcc gcaactttac tttcggggat aaacaaaaaa cattgactta caaaaacgct
      781 tgctactcaa aatgactttg ccaaacaaaa cgcaaacgaa ccagagtgtg atttttggaa
      841 aacctgacca agcgaaaaga gagcaccacc aacgaaactg agataatttt acgaactcaa
      901 cttaaccgaa atttctcaaa aagaaatagt accaattttg agaaggtgta aattaaacca
      961 cacctttatt tgatggtatc gtttaagagt tgccccagat tattgggatg gcacaaataa
     1021 taggctaatt gataagaaaa tcagccacaa tcaatagaat accaacagtt ccaataacca
     1081 tcaggaatac gttgaccttg cccttagacg tctcaagttt gaatgtcaag cctctagagg
     1141 agaaagagcc acctacaaga ccaaaaacac tcaatgcaac aagaaccaac ccaacattgt
     1201 tgtttaacca ttcaaacata tgaactcctt tgagatattt tgaaaagaat tgcagaaaaa
     1261 agacgacaat attttgagcg atgcgcctaa ctatggttta tacagcataa tgccgtaaaa
     1321 gcaccctata gacttcataa aacgattata caaccgtctc tccaaaccgg acatttccag
     1381 ccgtcttgcg gactgcaggt gactgtcagg ttggatactt tacttcgccc tctctaaatt
     1441 caaagccagt aatctctcga ggatcccttc atcggacagg gtgttctccc agccgtaggc
     1501 ggcgaagccg tccagccagc ctgtcctaga cgacagttca gggagaaagg taaaggctga
     1561 gtcttttcct ctcgccctcg aatagggggt aggggaagat gcccgccgcg tcttcacagg
     1621 gggaaggtca gaagaggtca tgtgcatgtt atcccggttc tatcgggacg ctttgttagg
     1681 cggcttctgt cttcggtatc agtag
//
LOCUS       DAOQJE010000841         1703 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_63129_length_1703_cov_3.841626, whole genome shotgun sequence.
ACCESSION   DAOQJE010000841 DAOQJE010000000
VERSION     DAOQJE010000841.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1703)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1703)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1703
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_63129_length_1703_cov_3.841626"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            475..>1703
                     /locus_tag="PLT08_17150"
     CDS             475..>1703
                     /locus_tag="PLT08_17150"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HNE06257.1"
                     /translation="MKKTTRSVAKMGGGDMRLSTKNGSSNSEGFYASIFDSIGSPILI
                     IDPQTAAILDANKAACKFYGYPHSDFIQKKILDLAAIPAKEAWQRIRKIAAGEITHMT
                     LQHRLANGEIRDVDVDLGRGNFEDRAVNIATVHDITEREQAKKKLQEGEDRYHSLFEN
                     MREGFALQEIIQDEAGRVVDFRFLDANAAYEEHTGQKLKEIIGHTIRETMPNADQAMI
                     ERYGNVALTGEPLDFEYHSKTFNRHIRVRSFRPQPNQFASIFEDITERKNAELLLQTV
                     NTELEKRVQERTAELGRIVERLNLATDAAKMGIWDWDIQHNVLIWDKQMYSLYGVKPE
                     DFGGAYEAWLNGIHPDDRETSEQVSEQARRGEIPYDVEFRVLWPDGSTHWLKADGQVF
                     WDEQGNPIRMLGVNYDIT"
BASE COUNT          488 a          362 c          410 g          443 t
ORIGIN      
        1 ataaacaaaa tttccctgtg tgatgcaaca gggaaaggaa gccagcgtca ctctttagtt
       61 tgctagttgg atagttgact agttatctag tctcgaatat ctaatctcta tcttctaaga
      121 aactctgcgg ggtaacgggt gcagttcccg tctctggtac tcgctactag tctcgaatta
      181 tcgaatatct agtatctttc tcttcacaac tcggaggtcc attcgacttc tgcgcttttg
      241 cctcgcttgc accgtcttcc gcttggctct ctggaaatcg ctttgaagtg tactcgtcct
      301 cgtcagcgtt tttggtttat gttgggcgag attttatgtc agagagggaa aagagtcaag
      361 gaggaaattt ggggaacaaa tcatgattca aggttttagg acaaagtatc cagtttggat
      421 aataagatgt acaataaaag taagtctgct ctcaatgcac tacaggagtg gtatatgaaa
      481 aaaactacca ggtctgtcgc aaaaatgggg gggggtgaca tgcgtctttc aacaaaaaat
      541 ggctcatcca attcagaagg cttttatgcc tctatctttg acagcattgg atcacccatc
      601 ttaattattg acccgcaaac tgccgcaatc ctggatgcga acaaagcggc ttgtaaattc
      661 tacggatatc cccactctga cttcatccaa aagaaaatcc ttgatctggc tgccatccca
      721 gccaaggaag cctggcagcg cattcggaag atcgcagcgg gtgaaatcac ccacatgact
      781 ttacagcacc gtctggcaaa cggtgagatc cgtgatgtgg atgtagatct ggggcgcggc
      841 aattttgaag atagggcggt gaacattgcc accgtgcatg acatcaccga gcgcgaacaa
      901 gcgaagaaga aattacagga aggtgaagac cgctatcact cgttatttga aaacatgaga
      961 gaaggctttg cgctgcaaga gatcattcag gatgaagccg ggcgtgtagt ggattttcgt
     1021 ttccttgatg caaacgcagc ctatgaagag catacaggac aaaaattaaa agagatcatc
     1081 ggtcacacca ttcgcgaaac catgccgaat gccgaccaag ccatgatcga acgatatggc
     1141 aatgtcgcac tcacaggcga accgttggac ttcgaatatc attccaaaac ctttaatcgc
     1201 catatccgtg ttcgatcttt ccgcccgcag ccaaatcaat ttgcatccat ttttgaagat
     1261 attactgaac gcaagaacgc ggaactttta ttacaaaccg tcaacacaga attggaaaag
     1321 cgtgtgcagg agcgtacagc cgaacttggt cgtattgttg aacgtctaaa tttagccaca
     1381 gacgcggcaa aaatgggcat ttgggattgg gatattcaac ataatgtgct gatttgggat
     1441 aagcaaatgt attccttgta tggtgttaag ccagaagatt ttggcggtgc ctatgaagcg
     1501 tggttaaacg ggattcaccc agatgaccgc gaaaccagtg agcaagtatc tgaacaagcc
     1561 cgaagaggtg aaatacctta tgatgtcgag tttcgagtct tgtggcctga tggctcaaca
     1621 cactggttga aagcagatgg tcaggttttt tgggatgaac agggaaatcc tattcgtatg
     1681 ctaggggtaa attatgatat tac
//
LOCUS       DAOQJE010000842         1702 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_63198_length_1702_cov_5.568913, whole genome shotgun sequence.
ACCESSION   DAOQJE010000842 DAOQJE010000000
VERSION     DAOQJE010000842.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1702)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1702)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1702
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_63198_length_1702_cov_5.568913"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..399
                     /locus_tag="PLT08_17155"
     CDS             <1..399
                     /locus_tag="PLT08_17155"
                     /inference="COORDINATES: protein motif:HMM:NF018649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1295 domain-containing protein"
                     /protein_id="HNE06258.1"
                     /translation="PVGMDIFMVVGFVVWLFGFVLEVTADRQKRAFNANPANKGKFIR
                     SGLWAWSRHPNYFGEIVLWIGVTIIALPVLRGWQWVTLISPLFITLLLTRLSGVPLLE
                     KRADDEWGGQAEYEAYKAKTPVLIPRAPKP"
     gene            complement(410..985)
                     /locus_tag="PLT08_17160"
     CDS             complement(410..985)
                     /locus_tag="PLT08_17160"
                     /inference="COORDINATES: protein motif:HMM:NF024769.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdeI/OmpD-associated family protein"
                     /protein_id="HNE06259.1"
                     /translation="MTKSLDLPTLPFESKKKFVDWLAKNHDKSAGLWLKIAKKATGIS
                     TVTYAEALDVALCYGWIDGQKGSFNEQYFLQKFTPRRPKSIWSKINVEKVEGLIASGE
                     MKPSGLKAVEAAKQDGRWDAAYSSQKNIVVPADFQSALEKNKKAKAFFETLTGAKRYS
                     FLFRIETAKKAETREKRIGQFVQMLAKGETF"
     gene            1094..1534
                     /locus_tag="PLT08_17165"
     CDS             1094..1534
                     /locus_tag="PLT08_17165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE06260.1"
                     /translation="MPYAFSALFFILALTTGIPALLQLRRMETIKRSSATTSGVVRMV
                     GRSNIGWNFLGEVGRSARPLVAFHVGDKEFEVEMTDNSGFMNRRYDTGNTVEVVYQKE
                     EPWKAYLTMERTFTRRDLWIALGEIALAMMFWGIGVYLKIPFST"
BASE COUNT          432 a          441 c          399 g          430 t
ORIGIN      
        1 ccagtaggca tggacatttt tatggtcgtc ggttttgtgg tgtggttatt tggctttgtg
       61 cttgaagtga ctgccgaccg acagaagcgc gctttcaatg ccaaccctgc caacaaaggc
      121 aagttcattc gcagcgggtt atgggcatgg tcacgtcacc cgaattattt tggtgagatc
      181 gtattgtgga ttggcgttac catcattgcg ctgcccgtgt tgcgcggttg gcaatgggtc
      241 acgcttattt ccccgctctt tatcactctt ctgctcaccc gtttaagcgg cgtgcccttg
      301 ctagaaaaac gcgccgatga cgaatggggc gggcaagcag agtacgaagc ctataaagcc
      361 aaaactccgg tgctcattcc gcgagcgccc aagccataaa ggcttgcagt taaaacgtct
      421 ccccttttgc cagcatctgc acgaattgac cgatgcgctt ctcgcgtgtt tcggctttct
      481 tcgccgtttc aattcggaac aagaacgaat agcgtttcgc gcccgtcaag gtctcgaaaa
      541 aggctttggc tttcttgttc ttctccaacg cggactgaaa atctgcgggc accacaatat
      601 tcttctgaga tgaatacgcc gcatcccaac gcccatcctg tttcgccgct tcgaccgctt
      661 tcaaccctga gggcttcatc tccccactcg cgatcagacc ttcaaccttc tccacattga
      721 tctttgacca aatactcttc ggtctgcgcg gtgtgaactt ctgcaaaaag tactgctcat
      781 taaagctgcc cttctgaccg tcaatccagc catagcacaa agcgacatcc aacgcttcag
      841 cataggtgac cgtggaaatt cccgtcgctt ttttagcgat cttcaaccac aagccagcag
      901 acttatcatg attcttcgcg agccagtcta caaatttctt tttggattca aagggtaagg
      961 tcggcagatc aagagatttt gtcattcttt aattttactt gaacccctgc cagtaaagaa
     1021 aagagagaaa tattaagttg aagatggatg taaaattcaa agggtatagc gattatagaa
     1081 aggaaaaaga acaatgcctt acgccttttc cgctttattt ttcatcctcg ctctaacaac
     1141 agggattcca gccctgctcc aattaagacg aatggaaacg atcaaacgct ccagcgcaac
     1201 cacctctggt gtagtccgca tggtgggacg ttctaacatc ggctggaatt ttctaggaga
     1261 agtaggcagg tctgcccgcc cgttggttgc atttcacgtc ggagataaag aatttgaagt
     1321 ggaaatgacc gacaacagcg gattcatgaa tcgccgctac gatacaggca acacggtcga
     1381 agttgtatat caaaaggaag aaccctggaa agcctatctt accatggagc ggacgttcac
     1441 ccgtcgtgac ctgtggatag ccctcggaga aattgccctc gctatgatgt tttggggaat
     1501 tggggtgtat ttgaaaatac cgttttcaac ctaatgaaaa tctcaaacac tctctatgtg
     1561 accaacccca aagactggcg cgagtggctc aagcagcatc acaaaaccga agcggaagtc
     1621 tggctggtgt accccaagaa agcaacgggc aagccgcgca tcgaatacaa cgacgccgtg
     1681 gagcaagccc tgtgcttcgg ct
//
LOCUS       DAOQJE010000843         1694 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_63796_length_1694_cov_6.754728, whole genome shotgun sequence.
ACCESSION   DAOQJE010000843 DAOQJE010000000
VERSION     DAOQJE010000843.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1694)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1694)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1694
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_63796_length_1694_cov_6.754728"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..375
                     /locus_tag="PLT08_17170"
     CDS             <1..375
                     /locus_tag="PLT08_17170"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HNE06261.1"
                     /translation="YGHKCGDLVLQSLAKFLQENTRRSDIVCRFGGEEFVILMPDAAV
                     EDAYERAERFRNNFALQIVEHEGKQVKSTFSAGVASFPVHANAGETLLSMADLALYQS
                     KANGRNRVTVFSPEIEEPAPAK"
     gene            412..1314
                     /gene="rocF"
                     /locus_tag="PLT08_17175"
     CDS             412..1314
                     /gene="rocF"
                     /locus_tag="PLT08_17175"
                     /EC_number="3.5.3.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR01229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004053 - arginase activity [Evidence
                     IEA];
                     GO_process: GO:0019547 - arginine catabolic process to
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginase"
                     /protein_id="HNE06262.1"
                     /translation="MQIDIIGVPIDLGADRRGVDMGPSAIRYSLLQKKLEDLGYTVND
                     EGNVEVPIAEMCKITNPKLKYIDCIIPMSRRVSGAVSTSIQAGNFPLVIGGDHSLSIG
                     SVRGAARNRKIGVIWVDAHADFNTAETTPSGNIHGMSLAALCGMGDPSLVQLWDEPVP
                     VIDPKKIAIVGARDLDEGEKRNLREAGAMVMGMEQVDRYGMVACLEKAIERISRDVDG
                     IYLSLDLDALDPEHAPGVGTPVPAGLTQREAHLACEMIGETGKLIGMDVVEVNPILDI
                     QNRTALLAVDFALSALGRRIWNGI"
     gene            1314..>1694
                     /locus_tag="PLT08_17180"
     CDS             1314..>1694
                     /locus_tag="PLT08_17180"
                     /inference="COORDINATES: protein motif:HMM:NF012673.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0046855 - inositol phosphate
                     dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HNE06263.1"
                     /translation="MKPTLSDLERLARGAGAILRDGYNKEHTVHYKGVIDLVTESDHA
                     SEAFLLGEIKSAFPESNILAEESGNIQGNNEDLWYIDPLDGTVNYAHHIPIFCVSIGF
                     STNGVLTLGAVYDPMRDEMFLAEKG"
BASE COUNT          437 a          444 c          398 g          415 t
ORIGIN      
        1 tacggacata aatgcggcga ccttgttctg caatctcttg ccaaattttt acaagagaat
       61 acacgccgca gtgacattgt atgtcgcttc ggcggtgaag aatttgtcat tctcatgccc
      121 gatgctgccg tagaagatgc gtacgaacgt gctgaacgtt ttagaaacaa ttttgccttg
      181 caaatcgttg agcacgaagg gaaacaggta aaatcaacct tctctgccgg ggtcgcatcc
      241 ttcccggttc acgccaatgc aggcgagacc cttcttagca tggcagacct cgcgctttat
      301 caatcaaaag ccaatgggcg aaatcgtgtt acagtctttt cacctgaaat agaagaaccc
      361 gccccggcaa aataaaattc ctcaaattca ttttagttta ggagaacttt catgcaaatc
      421 gatattatcg gtgtgcctat cgacttgggt gcagatcgcc gcggtgtgga tatgggtccc
      481 agcgcaattc gatattcttt gttgcaaaaa aaattggaag acctcggcta taccgtcaac
      541 gacgaaggca atgtagaagt gccaattgcc gaaatgtgta agatcaccaa cccaaagttg
      601 aaatatatcg actgcattat ccccatgtcg agacgcgttt ccggcgcagt atccacttcg
      661 atacaggctg gcaactttcc cttagtcatt ggaggcgatc actctttatc cattggctct
      721 gtacgcggcg cagcacgtaa tcgcaagatc ggcgtcatct gggtcgatgc acacgccgat
      781 ttcaatacag ctgaaaccac tccatccgga aatatccacg gcatgtcttt agcagctcta
      841 tgtggcatgg gcgacccaag cctcgtacag ttatgggatg aacctgttcc agtgattgac
      901 cccaaaaaga tcgccatcgt tggtgcacgc gacctcgacg aaggcgaaaa acgaaactta
      961 cgtgaagcgg gtgccatggt gatgggtatg gaacaggtcg atcggtacgg catggtggct
     1021 tgtcttgaaa aagcaatcga acgcatcagc cgcgatgtag atggaattta cctctctctc
     1081 gaccttgatg cgctcgaccc cgaacacgca cccggcgtag gtacacccgt ccctgcgggg
     1141 ctcacacaac gtgaagcgca tttagcttgt gaaatgatcg gcgaaaccgg aaagctcatt
     1201 ggcatggatg ttgttgaagt taatcccatc ctcgatattc aaaatcgcac cgccttgctc
     1261 gctgtggatt ttgccctctc tgctcttggt cgccgcattt ggaatggaat ttgatgaaac
     1321 ctactctctc tgatcttgaa cgactcgccc gcggagctgg agccattctc cgcgatggat
     1381 acaacaaaga acacacggtt cattacaaag gggtaattga tctggttact gaatctgatc
     1441 atgcctctga agcctttctg ctcggtgaaa taaaatccgc tttccccgaa agtaacatcc
     1501 tcgccgaaga aagcggaaat attcaaggca acaatgaaga cctctggtat atcgacccgc
     1561 tcgatggcac cgtcaactac gctcatcaca tccccatctt ctgcgtctct atcggattct
     1621 ccaccaatgg cgtgctgact ctcggcgccg tttacgaccc catgcgtgat gaaatgttcc
     1681 tcgccgaaaa aggc
//
LOCUS       DAOQJE010000844         1693 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_63878_length_1693_cov_3.943834, whole genome shotgun sequence.
ACCESSION   DAOQJE010000844 DAOQJE010000000
VERSION     DAOQJE010000844.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1693)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1693)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1693
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_63878_length_1693_cov_3.943834"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1045)
                     /locus_tag="PLT08_17185"
     CDS             complement(<1..1045)
                     /locus_tag="PLT08_17185"
                     /inference="COORDINATES: protein motif:HMM:NF015531.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HNE06264.1"
                     /translation="MSRKKNAMAYGFTALLVIQLACSSLSPIADEPVEVTVPLGPQVT
                     ETEQQTAVLESMLSQIDANYIYQKNSSVDLNALRTKYQAKIDKGLTSVEFNELMKQLE
                     AEFPTGDIVYATREERIENDTAASLVGYGGIGAFVNFQAEDEPHVVILDIIPGSPAEK
                     AGLKAHDSIYAVNGDPVLLEEGADVVQRIRGEAGTKVVLTVKTPGKSERDVEVTRAQI
                     TGTRGVIAEEIPNTDIGYIRFPTVGSATNASEVLDAMETFAKNSNLKGIVLDLRISGG
                     NSNFPLEDMLTLFLNDLSIDVYSLDKTNSFPIEGQDISGSQTIPLTVLIGENTNGTAE
                     IFAAAIQEKGRGIV"
     gene            complement(1103..1321)
                     /locus_tag="PLT08_17190"
     CDS             complement(1103..1321)
                     /locus_tag="PLT08_17190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE06265.1"
                     /translation="MTDTIQVGDRVQIRLDEKFGEREGWYGGTVVRIDPYSEHRSFYW
                     VRLDSEAQAILRVAEISVFNPKNIKKVS"
     gene            complement(1321..1506)
                     /locus_tag="PLT08_17195"
     CDS             complement(1321..1506)
                     /locus_tag="PLT08_17195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE06266.1"
                     /translation="MLNTIVKIFAVLLIASGIIWILQGVNILPGSFMTGDPQWAINGA
                     ITAAIGAGLFWFVSRKK"
BASE COUNT          398 a          424 c          396 g          475 t
ORIGIN      
        1 tgacgatgcc tctccctttt tcctgaatag cagcggcaaa aatttctgcg gttccgtttg
       61 tattttcacc gatcaatact gtaagcggaa ttgtctgtga accagagata tcctgccctt
      121 caatcgggaa ggagttggtt ttgtcgaggg aatacacgtc gatactcaaa tcgttgagaa
      181 agagagttag catatcctct aagggaaagt tcgaattccc gcctgaaata cgtagatcaa
      241 gaacaatgcc tttgaggttg ctgttcttgg cgaaggtttc cattgcatcc aatacttcgc
      301 tggcgttggt ggcgctccca actgtgggga agcggatata gccaatatct gtgtttggta
      361 tctcttctgc gatcactccg cgtgtgcccg tgatctgtgc gcgtgtaact tccacatcac
      421 gttcactttt ccccggcgtt ttgacggtga gcacaacctt tgtccctgct tcgccgcgga
      481 tgcgttgcac gacatctgca ccttcttcca aaagcacagg gtcaccattt acagcgtaga
      541 tactgtcgtg tgctttcaaa ccggcttttt cagcaggaga gccggggatg atgtcgagaa
      601 tcacaacatg gggttcatct tccgcttgga agttgacgaa agcgccaata ccgccatacc
      661 ctacaaggct ggcggctgta tcgttctcaa ttctttcttc ccgagttgcg taaacaatat
      721 ccccagttgg gaattcagct tcaagctgtt tcatcagttc gttgaattca acagacgtta
      781 aacctttatc gatcttcgcc tgatattttg tgcgcagagc atttaaatcc acgctactgt
      841 ttttctggta aatgtaattc gcgtcgatct gtgaaagcat gctttcaagg acggcagtct
      901 gttgctctgt ctctgtcact tgcggtccca aaggcactgt cacctctaca ggttcatctg
      961 cgatgggaga taaactggag caagccaatt gaatcacgag caatgctgta aacccatatg
     1021 ccattgcgtt cttttttcta ctcatctaaa gctccttccg gcttgattaa gacggcaata
     1081 cagatgcccc ctcttacgac tgctatgaaa ccttcttgat attcttggga ttaaagaccg
     1141 agatttcggc aactcttaag atggcttgcg cttcagagtc gagccgcacc caataaaaac
     1201 tgcgatgctc agaatacggg tcaatgcgga cgaccgtgcc gccgtaccag ccttctcgct
     1261 cgccaaactt ttcgtcgagg cgaatctgaa cccggtcccc gacctgaatg gtatctgtca
     1321 ttactttttg cgagagacga accagaataa cccggctcca atggcggctg taatggctcc
     1381 attgattgcc cattgtgggt caccggtcat gaagctgccg ggcaaaatgt tgacgccttg
     1441 cagaatccaa ataatgccag aagcgattaa gagtacagca aagattttta caatagtgtt
     1501 gagcatgatt ttttcctaat atggatttat ttataaaact ccctgcccaa gcggacaggg
     1561 agttgactta atgattttcg cctcgtactg gacctgttat ggattcggcg ttccctgaac
     1621 tgcgactccg acccaagtga agacacgtct ttcgctggtt gcgccggagg cgatccagat
     1681 cggtttgcct tgg
//
LOCUS       DAOQJE010000845         1690 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_64157_length_1690_cov_2.738226, whole genome shotgun sequence.
ACCESSION   DAOQJE010000845 DAOQJE010000000
VERSION     DAOQJE010000845.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1690)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1690)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1690
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_64157_length_1690_cov_2.738226"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1690)
                     /locus_tag="PLT08_17200"
     CDS             complement(<1..>1690)
                     /locus_tag="PLT08_17200"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HNE06267.1"
                     /translation="DITDLKQSEAALQASEANYRNLVESSESAIAVVDRNGKILFVNL
                     TGSDLWKDRNLVGKSIYDLFPPEYAERYSRAIHSIINTQIGMLDEIQSVVAGKMMWFR
                     LSVTPLKNLDGSISTLLINAWDITDRKLVEQSLTESEEKYRTLINSTTDGVFVAQDEQ
                     FVFCNAALPAMLGYAQEEFENIPFKQVLVPEYVDLWTDRFHRRIRDEKVLSNYQVQFQ
                     NKTGLQRIWVELRANKIEYGGRPAVLGIVRDITEQKNILAEIENLAKFPAENPGPVLR
                     IDLNGKLLYANDASFALLSEWTLEIGRPAPVEIQNIANVVIASERDKTIETIHNNRMV
                     VLDFIFLPQKGYINIYGRDITEQRHAEEEVHALQEFSQATIDALSGHLCVLDERGVII
                     SVNEAWRKFAEDNPPIPLNYGIGENYLMICENAKGDYENDARIVAHELRAVLEGKKSE
                     AYIEYPCHSPDEDFWFYLSIRRFESKGSTRLLLLHENITHRKQMETEILNGNQRFTEL
                     AENVSDIFWVSDPITRRNVYVNPAFSKITGMSMDAVENLPNDFLDIVLPEDRHT"
BASE COUNT          500 a          399 c          284 g          507 t
ORIGIN      
        1 atcgtatgcc tatcttctgg caaaacaata tccaagaagt cgtttggcag attttcaacc
       61 gcatccatac tcatcccagt tattttggaa aatgccgggt tgacataaac attacgtctt
      121 gtaatagggt ccgaaaccca aaagatatca gacacattct ctgccaattc agtaaaacgt
      181 tgattaccat ttaggatttc agtttccatc tgtttgcgat gagtaatatt ctcatgcaaa
      241 agaagcagtc tggttgaacc tttgctttca aatcggcgta tgctcaaata aaaccaaaaa
      301 tcctcatctg gagaatggca tggatattca atataggctt cgctcttctt tccctctaaa
      361 acagctcgta attcatgagc tactatacga gcatcattct cataatcccc tttagcattt
      421 tcacaaatca ttagataatt ttcaccaatc ccatagttca gtggaattgg tgggttatct
      481 tctgcaaact tccgccaagc ctcgttgacc gaaatgatga ccccacgttc atcaagcaca
      541 cacaaatgac ccgaaagagc atcaatagtt gcctgactaa attcttgtaa tgcatgcacc
      601 tcttcttcgg catgcctctg ttcagtgata tcacgcccat agatattgat ataacctttc
      661 tgaggcaaga aaataaaatc caatacaacc attctattgt tatgaatggt ctcaattgtt
      721 ttatctcgtt cagaagcaat caccacattg gctatatttt gaatttcaac cggcgcgggt
      781 cttcctattt caagcgtcca ctcgcttaat aaagcaaagc tcgcatcatt ggcatataac
      841 aatttaccgt tcaggtcaat ccgaagcaca ggtccagggt tttcagccgg aaatttggca
      901 aggttttcta tttcagctag aatgtttttc tgttctgtaa tatcgcgaac aatccctaaa
      961 acagcaggtc tcccaccata ctcgatctta ttggctcgca actcaaccca aatacgctgt
     1021 aagccagtct tattttgaaa ttgcacctga taatttgaaa gaaccttttc atcgcgaata
     1081 cgcctatgga atcggtctgt ccataaatcc acatactcag gcacaaggac ctgcttaaag
     1141 ggtatgtttt caaattcttc ctgagcatat cccaacatgg cgggtagtgc tgcattgcaa
     1201 aacacaaatt gctcatcctg cgcaacaaac actccatctg tagtggaatt gataagagtg
     1261 cgatacttct cttcactttc ggttaaagat tgctctacca gcttacggtc agtgatatcc
     1321 catgcattga taagcaatgt tgagatcgag ccatctaagt ttttgagagg ggttacgctc
     1381 agtcgaaacc acatcatttt gccggcaaca acactctgta tttcatccaa cattccaatt
     1441 tgtgtattga taatagaatg gatggctctt gaatatcgct cagcatattc tggcggaaat
     1501 aaatcgtaaa tagatttacc aaccaagttt ctatctttcc aaagatcact ccctgttaaa
     1561 ttaacgaata aaatctttcc atttctatct acaactgcaa tggcactttc agagctctca
     1621 accaaattac ggtaatttgc ctcactggct tgcaacgcag cttccgactg cttcaaatca
     1681 gtaatatcaa
//
LOCUS       DAOQJE010000846         1689 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_64211_length_1689_cov_3.536720, whole genome shotgun sequence.
ACCESSION   DAOQJE010000846 DAOQJE010000000
VERSION     DAOQJE010000846.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1689)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1689)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1689
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_64211_length_1689_cov_3.536720"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1030)
                     /locus_tag="PLT08_17205"
     CDS             complement(<1..1030)
                     /locus_tag="PLT08_17205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE06268.1"
                     /translation="MAKKVITSKKQSKKAKPVEDSIEVSKVGDGNAIAAGRGAKATSI
                     VINFFGGKWQLAISFIAIILAIGGYYLWTELFPAKMTGDFRVAVADFALTENSTEKGS
                     VIELSQGVYQKLDQSLSEIDKDLVVTVWSPDKVGKIEGNTPEERALSAEKIAHKIGAD
                     VVVYGLIDTSQPIWKITPEFYVVSDNFYEAEEITGHYQIGNTFNLEGQDSIKRRIELS
                     TKYEARAQVISRITIGLAYFALRNYHTSLTMFQSAEDISGLVDNQEDKVLHLLAGNAA
                     MKANEFDIAISELNKSLSIDPNYARPLITLGSVYYLQSLEPFEKTKKTDDIDLGLLDK
                     AIEQYKQAL"
     gene            complement(1067..1519)
                     /locus_tag="PLT08_17210"
     CDS             complement(1067..1519)
                     /locus_tag="PLT08_17210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE06269.1"
                     /translation="MTKKVVSKKTVASKKKIEGRGDIIRIQSVGYGAAVAAGRGAKAS
                     VISQESLSVIVEWMNQINKKIDSLPNVSQAEKEDIKQQVEKIGDEAQKGSKAEIGRLE
                     KLINSLNVMIPDIFDVITATLANPLAGIGLVIKKIGDKVKLEKDVSKV"
BASE COUNT          454 a          353 c          283 g          599 t
ORIGIN      
        1 ttaacgcctg tttgtattgt tcgattgctt tgtctaataa acctaggtca atatcatcag
       61 tttttttagt tttttcaaaa ggttccagcg attgtaggta gtagacactc cctaaagtaa
      121 ttaatggtct cgcatagtta ggatcaatgg aaagagactt gtttaattct gaaatcgcga
      181 tatcaaattc attggctttc attgctgcat ttccagccaa gaggtgcagt accttgtctt
      241 cttgattgtc aactaatcca gaaatatctt ctgctgattg aaacattgta agagaggtgt
      301 gataatttcg aagtgcaaaa tacgcaagcc caatggtaat tcttgagata acttgtgctc
      361 tagcttcata tttcgtactt agttctatgc gtcgcttgat actgtcttgc ccttccaagt
      421 taaatgtatt tccaatttga taatgccccg taatttcctc cgcctcgtaa aagttatctg
      481 ataccacata aaattcaggg gtaattttcc aaataggttg agatgtgtca ataagtccat
      541 acaccacaac atcagcacct attttgtgtg caattttttc ggcagataag gctctttctt
      601 ctggtgtgtt gccctcaatt tttccaactt tgtctggaga ccataccgtc acaacgagat
      661 ccttatcaat ctcggaaagt gattgatcta atttctgata cacaccttga gataattcaa
      721 ttactgagcc tttctcggta gaattttcag tcaacgcaaa atctgcaact gccacacgaa
      781 aatcgcctgt catttttgca ggaaacaatt ccgtccataa gtaataaccc ccaattgcaa
      841 gaataattgc aataaatgag attgctaact gccattttcc cccaaagaaa tttattacaa
      901 ttgaagtggc ttttgcgcca cgcccagcag cgatagcatt cccatctcct actttggaga
      961 cttcaattga atcctcaaca ggtttggctt tcttactttg tttcttgcta gttataacct
     1021 tttttgccat gcgtgttacc ttagagagtt caataatcag aaaatcttat actttggaga
     1081 catctttttc gagttttact ttatcaccaa tttttttaat tactagccca attcctgcta
     1141 acggatttgc gagtgtggct gtaataacat caaaaatatc aggaatcatt acattcaatg
     1201 agttgattag tttttcaagc ctcccaatct ctgcttttga acctttttga gcttcatccc
     1261 caattttctc aacttgttgt tttatatctt ctttttctgc ttgcgaaaca ttaggtagtg
     1321 aatcaatttt tttgttgatt tgattcatcc attcgacaat gactgataat gattcttggc
     1381 ttataactga cgcttttgcg ccacgtccag ctgctactgc tgcgccataa cctacacttt
     1441 gaattctaat aatatcgcct ctaccttcaa tttttttctt gctggcaacc gttttttttg
     1501 aaaccacctt ttttgtcata gtgattctcc tgtttcattt tcagtattat aaagcctgta
     1561 agaaaaataa attttgtggg atgattttct attttcacaa tcaattatga atgttggata
     1621 taaaaatatt cagcctgccc aacggtttgc gttacctgcg ctggggcggg gacggcgaag
     1681 ccgtccaac
//
LOCUS       DAOQJE010000847         1688 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_64295_length_1688_cov_3.155542, whole genome shotgun sequence.
ACCESSION   DAOQJE010000847 DAOQJE010000000
VERSION     DAOQJE010000847.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1688)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1688)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1688
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_64295_length_1688_cov_3.155542"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..553)
                     /locus_tag="PLT08_17215"
     CDS             complement(<1..553)
                     /locus_tag="PLT08_17215"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNE06270.1"
                     /translation="MSSDQYSIKVENLSKCYHVYNQPQDRLKQSVIPRLQKLIGRTPS
                     LYYHDFWALRDITFDVKKGETIGIIGRNGSGKSTLLQLICGTLTPTSGMVMTQGRIAA
                     LLELGSGFNLEFSGRENVYINGAILGLTKEEVDSRFDDIAAFADIGEFIEQPVKTYSS
                     GMVVRLAFAVQAMVDPDILIVDEA"
     gene            complement(543..1373)
                     /locus_tag="PLT08_17220"
     CDS             complement(543..1373)
                     /locus_tag="PLT08_17220"
                     /inference="COORDINATES: protein motif:HMM:NF013245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNE06271.1"
                     /translation="MNPHNEPNSSPIAMFSSFWQNRQLIWQMTRREISGRYRGSLLGL
                     AWSFLNPLVMLMVYTFVFSVVFQSRWGEDQIANRSDFAMVLFTGMIVYNFFAEIVNRS
                     PSLIITNVNYVKKVVFPLEILPWVSLGSVLFHSIVSLGVLLMLQLAANYSLPLTTLLF
                     PIVFMPLIFACMGIAWFLAALGVFVRDIGQVTGVFTTIMMFLSAVFYPLSALPQQYQF
                     WLRLNPLILIITECRNVLIFDNLPDWTALGFSWLTGLCMAVVGFWWFQKMRKGFADVI
                     "
BASE COUNT          591 a          356 c          324 g          417 t
ORIGIN      
        1 gtgcctcatc cacaataagg atatcaggat ccaccatagc ttgtacagca aaagcaaggc
       61 gcacgaccat gccgctagaa tatgttttta ccggctgttc tataaactcc ccaatatcag
      121 caaaagcagc aatatcatca aaacgggaat caacctcttc tttcgtaagt cctaaaatgg
      181 cgccatttat atatacattt tcacgaccgc taaattccaa attaaacccg gagccaagtt
      241 cgagcaatgc ggcaatacgc ccctgggtca taaccatacc acttgttgga gtaagtgtcc
      301 cacaaataag ttgaagaagt gtcgattttc ctgaaccatt ccgaccgata atacctattg
      361 tctcgccttt tttaacgtcg aaggtaatat cacgtaatgc ccaaaaatcg tgataataaa
      421 gactgggagt tctccctatt agcttttgca ggcgaggaat cacagattgt ttaagtcgat
      481 cctggggctg attatagaca tggtaacact tacttagatt ctcaaccttg atggaatatt
      541 gatcagatga catccgcaaa tcctttacgc attttttgaa accaccaaaa acccaccact
      601 gccatacaaa gcccagtgag ccatgaaaac ccgagcgcgg tccaatctgg tagattatca
      661 aaaatcaaaa cattgcggca ttctgtaatg attaatatca atggatttaa acgtaaccaa
      721 aactgatatt gctgcggtaa ggcactcaat ggataaaata cggcggaaag gaacatcatg
      781 atcgttgtga atacgccagt aacctgccca atatcccgta caaaaacgcc caaagctgcc
      841 aaaaaccaag caattcccat acaagcaaaa atgagcggca tgaaaactat cgggaaaagc
      901 agggtcgtca aaggtagtga ataattcgct gccaactgca acattaatag aacccccaag
      961 ctcacaatgc tatgaaaaag tacagatcca agagaaaccc acggcaaaat ttcaagagga
     1021 aaaacgacct tctttacata attgacattt gtaataatta aactagggga ccgattgaca
     1081 atctccgcaa agaaattata aacaatcatg cctgtaaaaa gaaccatggc gaagtctgac
     1141 ctattagcaa tctggtcttc gccccatctg gattgaaaga cgaccgaaaa aacaaacgta
     1201 taaaccatta acattaccaa cggatttaga aaagaccaag ccaagccaag taacgaccca
     1261 cgatacctgc cggaaatttc gcggcgagtc atttgccaga ttaactggcg gttttgccaa
     1321 aagcttgaga acatggcaat tggtgaagaa ttaggttcat tgtgaggatt catttaatta
     1381 atatgttttt ttagggaaaa gcagttcatt aaacttaaga ataaatcgta gaagttagta
     1441 gatttaatcc ataaaattca acggttgaat tttgccacaa cgcgtgaatt gcgtcaagaa
     1501 gagagactta aagcaatgca ttaataagta atatagatgc tattattttg tataattcac
     1561 caaaaaacta ccgtttttgg aaagtggtgt tttggtaata aaattatact cctacggggc
     1621 accaaagcgg agttgggtat cgaggtacag gttgttgtac caagggaacc agtaggaaga
     1681 attcaaga
//
LOCUS       DAOQJE010000848         1687 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_64354_length_1687_cov_6.750000, whole genome shotgun sequence.
ACCESSION   DAOQJE010000848 DAOQJE010000000
VERSION     DAOQJE010000848.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1687)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1687)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1687
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_64354_length_1687_cov_6.750000"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..505
                     /locus_tag="PLT08_17225"
     CDS             <1..505
                     /locus_tag="PLT08_17225"
                     /inference="COORDINATES: protein motif:HMM:NF012706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HNE06272.1"
                     /translation="NNAIKEYVPMIPVAHGGSGTAYRADVTNPQASPLTNEQFAFSAP
                     GDRDTFVWMQNAEPISMFCADESDGESLRACDQVMEALYGYEINGTATEPILAESCDP
                     SEDLTVWTCTLRQGVKFHDGSDFDANDVVATFNMGLNIDSPTHVGNSNIWSYYDYLWG
                     LMKKPGS"
     gene            589..1671
                     /locus_tag="PLT08_17230"
     CDS             589..1671
                     /locus_tag="PLT08_17230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNE06273.1"
                     /translation="MMIQYILRRVIASIPVLFGILLVTFVLARVIPGDPCRAILGEKA
                     TEEVCSRFIEEKGLNKPIVTQFAIYVKDMASGNLGDSFRLGLPVSRLLIERLPTTFEL
                     SLAALIVSILFGIPLGIISAVKHNTWLDVVTMVWANMGVSMPVFWLGLMLMYVFSLML
                     KDTPFWLPPSGRVSPGIPDTPFYELWGWVVPETGLWSAFLNFTGRLNILNSLLTQNWE
                     LLKDSIKHLILPALALGTIPMAIIARMARSAMLDVLGQDYIRTAKAKGLGRGGVIFKH
                     AFRNSLLPLVTVIGLSFGGLLGGAVLTETIFGLSGVGRTLYEAITARDYGIVQVFTLV
                     IAFVFVVINLIVDISYAYLDPRVRLN"
BASE COUNT          348 a          450 c          420 g          469 t
ORIGIN      
        1 gaacaacgca atcaaagaat atgttccgat gatccccgtt gctcacggtg gttccggcac
       61 cgcgtaccgc gctgatgtga ccaacccgca ggcgagcccg ctcaccaacg aacagttcgc
      121 tttctccgct cctggcgacc gcgacacctt cgtttggatg cagaacgccg aacccatcag
      181 catgttctgt gctgacgaat ccgatggcga atccctccgc gcttgcgatc aggtgatgga
      241 agccctctac ggctacgaaa tcaatggcac cgccactgaa cccatcctcg ccgaatcctg
      301 cgatccgagc gaagacctca ccgtctggac ttgtactctc cgccagggcg tgaagttcca
      361 cgatggcagc gatttcgatg ccaatgatgt ggttgccacc ttcaacatgg gcttgaacat
      421 cgactccccg acccatgttg gtaacagcaa catctggagc tactacgact acctctgggg
      481 cttgatgaag aagcccggtt cctaattcgt ttcggaacca agtttgtatg aaaaaaatac
      541 gggatggcgt aacccgccat cccgtattct cagggagcct tgcccatcat gatgatccaa
      601 tacatccttc gccgtgtgat cgcatccatt ccggtattat ttggcatttt gcttgttact
      661 tttgtgcttg cacgcgttat tcctggcgac ccttgccggg caattttggg ggaaaaagca
      721 acagaggaag tctgttctcg atttattgaa gaaaaagggc tcaataagcc catcgtcaca
      781 caatttgcga tctatgttaa ggatatggca agcggcaacc ttggcgactc cttccgcctt
      841 gggttgcctg tttcgcgttt gttgatcgaa cgcctcccta cgacctttga actgagtctg
      901 gctgccctga tcgtcagcat cctctttggc attcccttgg gcattatttc tgccgtaaag
      961 cacaacacct ggttagatgt ggtcaccatg gtgtgggcga acatgggcgt ttccatgccg
     1021 gtcttctggc ttgggttaat gctgatgtat gtgttctctt tgatgttgaa agatacacct
     1081 ttctggttgc ctccttccgg gcgtgttagc cctggtatcc ctgatactcc cttctatgaa
     1141 ttgtggggct gggttgtacc agaaacagga ctttggtctg ctttcttaaa ctttacgggt
     1201 cgtttgaaca tcctcaattc tctgctgact caaaattggg aattgttgaa agactcgata
     1261 aaacatctca ttttgccggc gttggcgctc ggaaccattc cgatggcgat catcgctcgt
     1321 atggctcgct ctgccatgct cgacgtgtta ggacaggatt acatccgcac agcaaaggcg
     1381 aaaggacttg ggagaggtgg cgtgatcttc aagcacgctt tccgtaattc cctcttgccg
     1441 ttggtgaccg tgataggttt gtcctttggc ggtctcttgg gcggagcggt tctcaccgaa
     1501 acgatctttg gtctttctgg tgtggggcgc actctctacg aagccatcac tgctcgtgat
     1561 tatggtattg tgcaggtctt tactctggtg atcgcattcg tcttcgttgt gatcaacctg
     1621 atcgtggata tttcttatgc ctatcttgat cctcgtgttc gcctcaacta aggaactcga
     1681 aaatgac
//
LOCUS       DAOQJE010000849         1687 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_64358_length_1687_cov_6.018995, whole genome shotgun sequence.
ACCESSION   DAOQJE010000849 DAOQJE010000000
VERSION     DAOQJE010000849.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1687)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1687)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1687
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_64358_length_1687_cov_6.018995"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..362)
                     /locus_tag="PLT08_17235"
     CDS             complement(<1..362)
                     /locus_tag="PLT08_17235"
                     /inference="COORDINATES: protein motif:HMM:NF014244.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HNE06274.1"
                     /translation="MKWQWKFGTFAGIDVYIHATFLLLIGWVGYSHWLEKNSWFDVLS
                     GIGFILALFLCVLLHEYGHALTARKYGIKTRDITLYPIGGVARLERMPEKPIEELWVA
                     LMGPAVNVVIAAILFAVL"
     gene            complement(567..>1687)
                     /locus_tag="PLT08_17240"
     CDS             complement(567..>1687)
                     /locus_tag="PLT08_17240"
                     /inference="COORDINATES: protein motif:HMM:NF012382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HNE06275.1"
                     /translation="YNQAVEEMLPGAKVRVNGRVMGMYASYSYLGLVGHPRINEAAKR
                     AVDKFGTGTNGVRTLAGTLTIHNELEETISNFKHAEAAITYTSGYVTNLTVISTLMGR
                     GDYVFSDKINHASIVDGCLMSGAEFRRFRHNDMEHLEGLLKNAPADVSKLVIADSVFS
                     MDGDIIDLPKIVELSKKYNAWLMIDEAHSVGVLGKTGTGIEEHFNMYGSIDIKMGTLS
                     KTIPSVGGYVAGKKELIDYLRHGSRAYIFSAALPPAQAAAANEAFKVILDEPERLDRL
                     NANTQQFIGGLKSMGFDTLLTETAIVPVLCGTDEKAMELTKRCQEQDVFVLPVVSPAV
                     QEGMSRLRATVTAAHEPADIEHAMDVIYQSGKAMGMVK"
BASE COUNT          404 a          429 c          447 g          407 t
ORIGIN      
        1 aaaagaaccg caaaaagaat ggcggcaatc accacgttca cggcgggtcc catcaacgcc
       61 acccacaatt cctcgatcgg tttctcaggc atgcgttcca aacgcgccac gccaccaatc
      121 gggtagagtg tgatatcgcg cgtcttgatt ccgtatttac gagcggtcag cgcgtgccca
      181 tattcatgca agagtacgca taaaaagagc gcgagaataa agccaatgcc gctcaacaca
      241 tcgaaccagc tatttttttc caaccaatga ctatacccaa cccacccgat caaaagtaaa
      301 aaggtggcat ggatgtagac atcaattcct gcaaatgttc caaatttcca ttgccatttc
      361 atagtttgct ccttgtaacg ttgacggcat tgtacaagga atttattaga aaaatgttat
      421 aaaaattcta tcaagaacta tcaggatgaa aaccccgctt cccactccgc ttgggcttgg
      481 tctgtctctc caagcagcag cgaacgccga taaaaaaatc gcctgtcgat aggacaggcg
      541 attttcaact ttatctaact atctaattac ttgaccattc ccatggcttt gccagattgg
      601 tagatcacat ccatggcgtg ttcaatatct gcaggttcgt gtgccgcagt gaccgtggca
      661 cgcaagcgag acatgccctc ttgcactgca ggcgagacga cgggcagaac gaagacatcc
      721 tgctcttggc agcgtttggt caattccatg gctttttcat cggtgccgca cagcacaggg
      781 acgatggcag tttcggtgag caaggtatca aagcccatac tcttcaagcc accaataaat
      841 tgttgagtgt tggcgttcaa gcggtcgagg cgttcgggtt catcgagaat gaccttgaag
      901 gcttcgttcg cggcggctgc ctgagcaggt gggagcgctg ccgagaagat gtacgcacgg
      961 ctgccgtggc gcaggtaatc gatcagttct tttttgccag ccacatatcc gccaaccgaa
     1021 gggatggtct tgctgagggt gcccatcttg atgtcgatgg agccgtacat gttgaagtgt
     1081 tcttcaatgc cggtgccggt cttgccgagc acgcccaccg agtgcgcttc gtcgatcatg
     1141 agccaggcgt tgtatttttt gctaagctcc acgatcttgg gcaggtcgat gatatcgccg
     1201 tccatgctga agaccgagtc cgcgatgacg agcttggaga catcggcagg agcgttcttg
     1261 agcaagcctt cgagatgttc catgtcgttg tggcggaaac ggcggaattc cgcaccagac
     1321 atcaaacaac cgtccacaat ggaggcgtgg ttgatcttgt ctgagaagac gtagtcgccg
     1381 cgtcccatca aggtagagat gacggtcagg ttggtgacat acccagaagt ataggtaatg
     1441 gcggcttctg catgcttgaa gtttgaaatg gtttcttcga gttcgttgtg aatagtgagg
     1501 gttccagcca aggtgcgcac gccgttggtg ccggtgccga atttgtctac ggcgcgtttg
     1561 gcggcttcgt taatgcgtgg gtgaccgacc aagcccaagt aggagtaaga ggcgtacatg
     1621 cccatcacgc gcccatttac gcgcactttt gcgccgggga gcatttcttc cacggcttgg
     1681 ttgtaaa
//
LOCUS       DAOQJE010000850         1687 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_64359_length_1687_cov_5.928922, whole genome shotgun sequence.
ACCESSION   DAOQJE010000850 DAOQJE010000000
VERSION     DAOQJE010000850.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1687)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1687)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1687
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_64359_length_1687_cov_5.928922"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(91..684)
                     /locus_tag="PLT08_17245"
     CDS             complement(91..684)
                     /locus_tag="PLT08_17245"
                     /inference="COORDINATES: protein motif:HMM:NF037740.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE06276.1"
                     /translation="MHQYQTDDNAKTNFLIFAVLLAVGLAYLFGKAISALQIQIPWYV
                     ESPSILGFFGAIYWLYDKYLWKTKWVQMIDWIKTPNISGKWDVEIRTSHDSFTEKTSG
                     KAIIRQSAFRISIALETKSSISSSIHAALMRTEKVSEYELTYNYINHPKADSIETMSI
                     HMGTTRVSLSDDGKQMDGEYYTGRDRQNFGRITLLRT"
     gene            complement(689..1561)
                     /locus_tag="PLT08_17250"
     CDS             complement(689..1561)
                     /locus_tag="PLT08_17250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase"
                     /protein_id="HNE06277.1"
                     /translation="MTISETQLETWSHQGGVDAAQNTHTAIRNALNDYSWKSGVSYDD
                     YLQGSYRNTTNIYGNSDVDLAVELTSTFYHNELTESQKSALGFSSATYSINNFREDII
                     SILRNRFGTAYVDTSGGKSIKVLPNNGRLKGDVVVSAKYRHYEGTRLVAEGITFWNTK
                     TGEQIVNYPKIHLENGVTKHANTSQWYKPTVRIFKNARERICKNKTWLLGKFPSYFIE
                     CLLYNVPNAKYGQSYQDTFCNVVNWINDALTADPSQFVCQNKMFYLFGSSSTQWNVND
                     AKTFISELTSLWNS"
BASE COUNT          465 a          312 c          333 g          577 t
ORIGIN      
        1 tgtaaaactg cttgggatgt gcgcagaatc cccagcgtca ggtgcacgct ttgttggcac
       61 gctcttggtt attaaagatg cgcccatttt tcaagttcgt aataaggtaa tgcgtccaaa
      121 gttttgacgg tctcttcctg tgtaatattc tccatccatt tgcttgccat catctgaaag
      181 tgaaacgcga gttgttccca tgtgtatact cattgtttca attgagtctg cttttggatg
      241 gttaatgtaa ttataggtca attcgtattc gctaactttt tctgttcgca tcaaggctgc
      301 atgaatacta cttgatatag atgatttagt ttcaagtgca atagaaatcc tgaatgctga
      361 ttgacgaatg atggcttttc ctgaggtctt ttctgtaaat gaatcatgag atgttcgaat
      421 ctcaacatcc cattttcctg aaatgttggg agtcttaatc caatctatca tttgaaccca
      481 ttttgttttc caaagatatt tgtcgtacaa ccagtaaatt gctccaaaaa atcctagaat
      541 tgatggggat tcaacgtacc agggaatttg aatttgtaaa gcacttattg cttttccaaa
      601 caagtaggca agcccaactg ccaataaaac agcaaatata aggaagtttg ttttagcgtt
      661 gtcgtcagtt tgatattggt gcattttgtt atgaattcca gagagaagtc aattcagaaa
      721 taaaagtttt agcatcattg acattccatt gtgtggaact ggagccaaaa agatagaaca
      781 ttttgttttg acatacaaat tgcgatgggt ctgctgtaag agcgtcgtta atccagttaa
      841 ctacattgca aaaagtgtct tggtaagatt gaccatactt cgcgtttggt acattgtaaa
      901 gcaaacattc gataaaataa gatggaaact tacccaaaag ccaagttttg tttttacata
      961 tacgttctcg cgcgtttttg aaaattcgta cagttggctt gtaccattgt gacgtatttg
     1021 cgtgtttagt tacaccattt tctaaatgaa tttttggata gttgacaatt tgttctcccg
     1081 tttttgtatt ccaaaatgtt atgccttcgg caactaacct tgtaccttcg tagtgtctat
     1141 attttgccga aactactaca tcacctttga ggcgtccatt atttggtaat acctttatcg
     1201 atttaccacc tgatgtgtca acatatgcag tcccaaaacg gtttcttaga atggaaatta
     1261 tatcttcgcg aaaattattg atgctataag ttgcggacga aaagcccagg gcgctctttt
     1321 gagattcggt caactcattg tggtaaaaag tgcttgttaa ttcaactgct aaatcaacgt
     1381 cgctattgcc ataaatattt gtagtattgc gataagagcc ttgtaaatag tcatcgtaag
     1441 agacaccact tttccaagaa taatcattca aagcattgcg aattgcagta tgagtatttt
     1501 gggcggcatc tacaccacct tgatgtgacc atgtttcaag ttgggtttca gagattgtca
     1561 tgcttatttt cccttccttt attttattca ctcaattcaa ggtgcagata tatattttgc
     1621 aacgtggatt ttttaccttg ttatgcacgg ggcgtgccaa cggcatgcgt tacctgcgct
     1681 ggggcgg
//
LOCUS       DAOQJE010000851         1687 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_64361_length_1687_cov_5.389706, whole genome shotgun sequence.
ACCESSION   DAOQJE010000851 DAOQJE010000000
VERSION     DAOQJE010000851.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1687)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1687)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1687
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_64361_length_1687_cov_5.389706"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1687)
                     /locus_tag="PLT08_17255"
     CDS             complement(<1..>1687)
                     /locus_tag="PLT08_17255"
                     /inference="COORDINATES: protein motif:HMM:NF024583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HNE06278.1"
                     /translation="IINSVQAALAAELNIQGIYDAVGDKIREIFNNKDLTIRIYDPKT
                     KLLHIPYANQGGQRLEVAPLPIDKGFSAHVLRTRETIVINENMLEEEKKYGAYTLPGT
                     ESEKSLVFVPLIAGDQVRGMINLVSMEEHAFSESDVRLLQTLANAMSVSLENARLFDE
                     TQRLLKITEERNAELAIINSVQAALAAELNIQGIYDAVGDKIREIFHNTDMNIRIQDP
                     QTNMTLYPYFYENGKRLFLEPQPYRVQGFTHYVLSTRETVVINENLLEEEKKYGSFTL
                     PGTESEKSLVFVPLVTGDKARGLINLSSMEEHAFSESDVRLLQTLANAMSVSLENARL
                     FDETQRLLKITEERNAELAIINSVQAALAAELNIQGIYDAVGDKVHEIFNHRDMNIRI
                     YDAKTNLVHIPYIYENGERIAVEPNPLGEQGFEAYVIRTRETLVINENMEAAMAKVGS
                     YTIAGTAAAKAQVFVPLTVGDQVRGMLNLVDNDHEHAFSESDVRLLTTLANSMSVALE
                     NARLFDETQRLLKITEDRAAELAIINSVQEGLASKLDMQAIYDLVGNKVCEIF"
BASE COUNT          343 a          412 c          469 g          463 t
ORIGIN      
        1 ttgaagatct cgcagacctt gttgcccacc aggtcgtaga tggcttgcat atccaatttg
       61 gaggcaagcc cttcctgcac cgagttgatg atggcaagtt cggcggcgcg gtcttcggtg
      121 atcttgagca gacgttgggt ctcgtcgaag agacgcgcat tctcgagcgc gacgctcatg
      181 ctgtttgcaa gcgtggtcag cagacgcacg tcgctttcgc tgaaggcgtg ttcatggtcg
      241 ttatccacca aattcaacat accgcgcact tggtcaccga cagtgagagg cacaaatacc
      301 tgcgctttag cggctgccgt gcctgcaatg gtgtaactcc ccaccttcgc catcgctgct
      361 tccatatttt cattaatgac cagggtttcg cgtgtacgga tgacataggc ttcaaaacct
      421 tgctcgccca gcggatttgg ttccactgca attcgttcgc cattctcata gatgtagggg
      481 atgtggacta aattcgtctt ggcatcatag atgcggatgt tcatatcgcg gtgattgaaa
      541 atctcatgca ctttatcacc gaccgcatca taaattccct gaatgttgag ttcggcagcg
      601 agggcggctt gcacgctgtt gatgatggcg agctcggcat tgcgttcttc ggtgatcttg
      661 agcagacgtt gggtttcatc gaagagacgc gcattctcga gcgagacgct catggcgtta
      721 gcaagcgttt gtaagaggcg cacgtcgctt tcgctgaagg cgtgttcttc catgctactg
      781 aggttgatta acccgcgcgc cttgtcgcct gttacaagcg gcacgaacac caacgatttt
      841 tcagattcgg ttccgggcag ggtgaaactg ccgtacttct tttcctcttc caacaggttt
      901 tcgttgatga ctaccgtttc gcgggtgctt agaacataat gggtaaagcc ttgcactctg
      961 tagggttgtg gctcaaggaa gagtctcttt ccattttcat aaaaatatgg atacagggtc
     1021 atatttgtct gcgggtcttg aatacggata ttcatatccg tattgtggaa gatctcgcgg
     1081 atcttatcgc cgaccgcatc gtagatgccc tgaatgttga gttcggcagc gagggcggct
     1141 tgcacgctgt taatgatggc aagctcggcg ttgcgctctt cggtgatctt taagaggcgc
     1201 tgggtttcat cgaacaggcg ggcgttctca agtgagacgc tcatggcatt ggcaagcgtt
     1261 tgcaagaggc gcacgtcgct ttcgctgaag gcgtgttctt ccatgctgac gaggttgatc
     1321 atgccgcgca cctggtcccc tgcgatcagc ggaacgaaca ccaacgattt ttcagattcg
     1381 gtcccgggca gggtatatgc cccgtatttc ttttcctctt cgagcatgtt ttcattgatg
     1441 acgatcgttt cacgggtacg aaggacatgc gccgaaaagc ctttgtctat tggcaacggg
     1501 gcgacttcaa gacgctgccc gccctggttt gcgtagggga tatgaagtaa ttttgtttta
     1561 gggtcgtaaa tgcggatggt caaatcttta ttgttaaaga tctcgcggat cttgtcgccg
     1621 accgcatcat aaatgccctg aatgttgagc tcggcggcga gggcggcttg cacgctgttg
     1681 atgatgg
//
LOCUS       DAOQJE010000852         1686 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_64436_length_1686_cov_6.520540, whole genome shotgun sequence.
ACCESSION   DAOQJE010000852 DAOQJE010000000
VERSION     DAOQJE010000852.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1686)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1686)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1686
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_64436_length_1686_cov_6.520540"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            60..1547
                     /locus_tag="PLT08_17260"
     CDS             60..1547
                     /locus_tag="PLT08_17260"
                     /inference="COORDINATES: protein motif:HMM:NF024640.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prenyltransferase/squalene oxidase
                     repeat-containing protein"
                     /protein_id="HNE06279.1"
                     /translation="MLNIDEEVKKVLNEVGPGRMSSTAYDTAWVARLGEIDKDLSDQA
                     VNWLLENQLPDGSWGASDVFYYHDRVISTLIAMIALKEHGGNSHHQAQVEKGVEALER
                     ITSDGEHSFASDLNGATVAFEMVAPILLAEAKELGIIKQQGERILGRLSNMRQEKMRK
                     LAGYKINRYVTPAFSAEMAGIDGQHMLEVENLQEANGSIGHSPSATAYFALRVKPGDA
                     KAIEYLKSSVAKNGGAPDLYPFDVYERAWVLWNLSLTDYFEGDKRSFANPILNYLAAA
                     WKPGQGIGFSKDYSVIDGDDTIITCELFNKFGYPTDLNAILSFEEEEYFRCYQLEVGI
                     SPSVNIHALMTLRDFDYPLEHPSVRKVFQFLETQKSGDFWVDKWNSSSFYTSSHYVIA
                     CAKFKNAIAQPTVDWILKNQRADGSWGTFGSTTEETAYCLQALSIWSRHVRSVPQDVI
                     RRGRTWLLEHYDLPHSPIWIGKGLYTPQWVSCSAILSAMLLTVSY"
BASE COUNT          448 a          351 c          422 g          465 t
ORIGIN      
        1 ggtgagatgc gcggaggaat ttggtacgcc accccacacc ttaaaaagta gagttcatca
       61 tgcttaacat cgacgaagaa gtaaaaaaag tactgaacga ggtgggaccc ggacgaatga
      121 gtagcactgc ctacgacacc gcttgggtcg cacgcttagg cgaaattgat aaagacttaa
      181 gcgatcaagc tgtcaactgg ctgcttgaga atcaattgcc agacggtagt tggggcgcca
      241 gcgacgtgtt ctattatcac gaccgtgtca ttagcacgct gattgccatg attgcgctta
      301 aggaacatgg tgggaattct catcatcaag ctcaagttga aaagggtgta gaagcattag
      361 agcgtatcac ttctgatgga gagcacagct tcgcttcaga cctaaacggt gccacagttg
      421 cttttgaaat ggtcgctccg atcttgcttg cagaagccaa ggagctaggc attatcaagc
      481 aacagggaga gcgaattcta ggtcgcctga gcaacatgcg gcaagagaag atgcgcaaac
      541 ttgctgggta taagatcaac cgctatgtca ctccagcctt ttcggctgag atggctggta
      601 tagatggtca acatatgctt gaggttgaga acttacagga agcgaatggt tccattgggc
      661 atagcccatc tgccacagct tattttgctt tgcgggtaaa acccggagat gcaaaagcga
      721 tagagtatct aaagagttct gtggcgaaaa atggcggtgc gcctgatctc tatccgtttg
      781 atgtgtatga acgggcatgg gtcttatgga atttatctct aaccgattat ttcgaaggcg
      841 ataagcggtc ttttgctaac cctattttga actaccttgc agcagcatgg aaaccggggc
      901 aggggattgg tttttctaag gattacagtg ttattgatgg cgatgacacc atcattactt
      961 gtgagttgtt taataaattt ggctatccaa ccgatcttaa tgccatattg tcttttgaag
     1021 aagaagagta cttccgctgt tatcaattgg aggtgggtat ctctcccagt gtaaatatcc
     1081 atgcgttaat gacattaagg gattttgact atccgctcga acatcccagc gtcagaaaag
     1141 tgtttcagtt tttagaaacc caaaaatctg gagacttttg ggtcgataaa tggaattctt
     1201 cttcattcta tacctcgtct cattatgtga ttgcctgtgc caagtttaag aatgccattg
     1261 ctcagccaac agtagattgg atactaaaga atcaacgagc cgacggctct tggggtacgt
     1321 ttggctctac aaccgaagaa acagcctact gccttcaggc gctttccata tggtcgaggc
     1381 acgtccgtag cgtaccgcaa gatgtgattc gacgaggaag aacctggttg cttgagcatt
     1441 acgacttgcc gcattccccg atttggatcg gcaagggact gtacaccccg caatgggtct
     1501 cttgttcagc tatcttaagt gcaatgttgt taacggtttc atattaactg ttttatgttt
     1561 taatcatttt gtctgtattc tctacgtaaa catggtggtg cctatgatcg cgaatctaca
     1621 aaacaattgg gagcgaatta ataagtttga tgggtttgac caggatattg cccggcgtgg
     1681 tcgcct
//
LOCUS       DAOQJE010000853         1682 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_64733_length_1682_cov_5.872157, whole genome shotgun sequence.
ACCESSION   DAOQJE010000853 DAOQJE010000000
VERSION     DAOQJE010000853.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1682)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1682)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1682
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_64733_length_1682_cov_5.872157"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(206..406)
                     /locus_tag="PLT08_17265"
     CDS             complement(206..406)
                     /locus_tag="PLT08_17265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003224033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HNE06280.1"
                     /translation="MNNNLKVLRAEKNWSQADLAEKLNVSRQTVNALETGKYDPSLPL
                     AFKIADLFGKKIEEIFFPEKEN"
     gene            complement(403..792)
                     /locus_tag="PLT08_17270"
     CDS             complement(403..792)
                     /locus_tag="PLT08_17270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE06281.1"
                     /translation="MNQDQVQKRYYKEFFIAIGAYVVILIGSISILKYLELPKSAQFI
                     VAILPIIPILFVIIAIMRALRDSDELQQKIQSYAIMFSAVATGLITFSYGLLENVGFP
                     PFPTIWVLPMMFMFWGVSLSYFWKKYQ"
     gene            complement(797..949)
                     /locus_tag="PLT08_17275"
     CDS             complement(797..949)
                     /locus_tag="PLT08_17275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE06282.1"
                     /translation="MNTKNLGSTIAIAAGVGTAVSVSSHSIAVGVGVGVAIFIAFSVF
                     GNFKKE"
     gene            1081..1497
                     /locus_tag="PLT08_17280"
     CDS             1081..1497
                     /locus_tag="PLT08_17280"
                     /inference="COORDINATES: protein motif:HMM:NF022698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3052 family protein"
                     /protein_id="HNE06283.1"
                     /translation="MDTGYSGTPLYKKLGFTKAPLRVLTINVPREYKSWLGELPKGVG
                     LASNVRSPVQAAHVFATEKAFLDAVLSTLRTELAQDGFVWVSWPKKTSKVKTDITEDT
                     IRELALPLGFVDIKVCAVSEVWSGLKLVIRKSERKE"
BASE COUNT          446 a          316 c          447 g          473 t
ORIGIN      
        1 ggaaattgtg agcttggatt cttttcggga taaagaaacg cctccgcttc gacgggggag
       61 ctgattccgc gtcggaggag agtctgtgca atgaggggag gcaagcccaa gtcttggaaa
      121 gaggcgggga tgtctacagg ttgagggtcg agccagcgag tcataaaaat agaaccatcc
      181 aaaaaagatg gttctattgt agcagttagt tttctttttc cggaaaaaat atttcttcga
      241 tctttttccc gaatagatcg gcgatcttga atgcaagcgg aaggctgggg tcgtacttgc
      301 cggtttcaag ggcgttgacg gtttgccttg agacattcaa tttctctgca aggtcggctt
      361 gagaccagtt cttttctgcg cgcaagacct tgagattgtt gttcattggt atttcttcca
      421 gaagtagcta aggctaaccc cccagaacat gaacatcatg ggcaataccc aaatggtagg
      481 gaagggtgga aagcctacgt tttcgagcaa gccatagcta aatgtgatca agccggttgc
      541 cacagcagag aacataatgg cataagactg aattttttgc tgaagttcgt cgctatcgcg
      601 tagtgcgcgc atgatggcga tgatgacgaa gagaataggg atgatgggca aaatggcaac
      661 gatgaattgg gcagatttgg gcaattcgag gtatttaaga atgctgatac tgccgatcaa
      721 aataaccaca taggcaccaa tggcgataaa gaactctttg taataccttt tttgaacttg
      781 atcctgattc atttgattac tcctttttga aatttccaaa cacgctaaat gcgataaaaa
      841 tagcgacgcc taccccaaca ccgactgcaa tactgtgcga agagacgctg accgcggttc
      901 ccactccggc ggcgatggcg attgtgcttc ctagattttt tgtgttcaag ttacatctcc
      961 ttgtgtaaag tttgcttgac tttttgtatt gtaaagtaag ctttacttta agtcaagggt
     1021 ttttgactga cacttttgga gatgcgagta cacttaaagg gatacgaaag gattattggt
     1081 atggacacag gttattcagg aaccccactt tataagaaat taggttttac caaagcgcca
     1141 ttacgagttt tgacgattaa cgtccctcgc gaatacaaga gttggctggg ggaattgcca
     1201 aagggtgtag ggctggcttc gaacgtccgc tcgcctgtgc aggcggcgca tgtgttcgct
     1261 acggagaagg cttttttaga tgcggtcctt tccactttgc gaacggagct tgcccaagat
     1321 ggttttgttt gggtatcttg gcccaagaaa acctcaaaag taaaaacaga cattactgaa
     1381 gatacgatcc gtgagcttgc tttgccatta ggttttgtgg atataaaagt atgtgccgtg
     1441 agcgaagtgt ggtctgggct taagttggtg attcggaagt ctgaacgaaa agagtagttg
     1501 cattcccata caaaattttg taccatgggg atgctcttaa cgttatttta ttgtcaaagg
     1561 agaatgtgca atatgaagaa cgtattgaaa tggatcggga tcgtagtagg gagtttggta
     1621 gggctggtgc ttgtggcagg ttttgtcatg tttcttgttg ggaatgcccg cctgaacaag
     1681 ac
//
LOCUS       DAOQJE010000854         1682 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_64734_length_1682_cov_5.633067, whole genome shotgun sequence.
ACCESSION   DAOQJE010000854 DAOQJE010000000
VERSION     DAOQJE010000854.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1682)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1682)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1682
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_64734_length_1682_cov_5.633067"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1030
                     /gene="hppD"
                     /locus_tag="PLT08_17285"
     CDS             <1..1030
                     /gene="hppD"
                     /locus_tag="PLT08_17285"
                     /EC_number="1.13.11.27"
                     /inference="COORDINATES: protein motif:HMM:TIGR01263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003868 - 4-hydroxyphenylpyruvate
                     dioxygenase activity [Evidence IEA];
                     GO_process: GO:0006559 - L-phenylalanine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006572 - tyrosine catabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="4-hydroxyphenylpyruvate dioxygenase"
                     /protein_id="HNE06284.1"
                     /translation="AKHAMYYWWKAFGFKPVAYSGLETGNRDFASYVLESGQARFVVS
                     APYSPSSPLASHHMVHGDGVKVIALEVDDVEKAWRETTSRGGKSAWAPREEKDDHGIY
                     RSSAIYTYGETIHAFIDRDDYKGTFAPTYRPLKHEAESTGLAAIDHIVGNVQLGKMNH
                     WVNFYHQVMGFRQLMHFDDKDISTEYSALMSKVMQNGNGRVKFPINEPADGKRKSQIE
                     EYLDYYMSPGAQHIAIITGDILSTVESLRKNGVEFLRVPDTYYEMLPDRVGKIKEDFK
                     TIHELGILVDKDDEGYLLQIFTRPIQDRPTMFIEIIQRHGAQGFGKGNFKALFESLEL
                     EQERRGNL"
     gene            1229..>1682
                     /locus_tag="PLT08_17290"
     CDS             1229..>1682
                     /locus_tag="PLT08_17290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE06285.1"
                     /translation="MNKSKSILGLVLGLFLVLACGAPAATPDVAAIVTQTLQALTAIP
                     TEAQQQAEVLPTAESSQQLTPNGTPVDTAEISFFIPNGVANDASSTMTANVEYPYINP
                     SLGDMPQHASITLNLYAVTKSIHEAQILIFRASEYSQYSESNAATISQL"
BASE COUNT          458 a          465 c          350 g          409 t
ORIGIN      
        1 cgccaaacat gccatgtatt attggtggaa ggcatttggc ttcaaacctg ttgcatattc
       61 agggttagaa actggcaacc gcgacttcgc ctcgtatgta cttgaatcag ggcaagcgcg
      121 gtttgttgtt tctgcacctt attccccatc cagcccactc gcatcacacc acatggttca
      181 tggcgatggt gttaaagtca ttgcgctgga agtggatgat gttgagaaag cctggcgtga
      241 aaccaccagc cgcggaggaa aatctgcctg ggcaccgcgt gaagaaaagg atgatcatgg
      301 catttaccgc agttctgcca tttacaccta cggcgaaacc attcacgcct ttattgaccg
      361 tgatgactac aaaggtacct tcgccccaac ctatcgtcca cttaaacatg aagcggagtc
      421 taccgggctt gctgccattg accacattgt tggcaacgtg caactcggca aaatgaatca
      481 ctgggtaaat ttttatcatc aggtaatggg cttccgtcaa ctcatgcact tcgacgataa
      541 agacatctcc accgaatact ccgccctcat gtccaaggtc atgcaaaacg gaaatggacg
      601 cgtcaagttc cccatcaatg aacctgcaga tggaaaacga aagagtcaga tcgaagaata
      661 tctcgactat tacatgtctc ccggtgcgca acatattgcc ataatcacag gcgatattct
      721 cagcactgtt gaaagtctcc gtaagaatgg tgtcgaattt ttacgcgtgc cagatactta
      781 ctatgaaatg ctgccagacc gtgttggcaa gatcaaagaa gatttcaaaa ccatccacga
      841 actcggcatt cttgtggata aagatgatga aggctatctg ctgcaaatct tcacccgtcc
      901 tattcaagac cgccctacca tgttcattga gatcattcaa cgccatggcg cgcagggttt
      961 cggcaaaggc aacttcaaag ccttattcga gtctcttgag ttggagcaag aacgccgcgg
     1021 caatctttag cgcatgagct tctgcggata aaaatcatcg cctcacacca gacccgctgc
     1081 ctgtatccca tttcagacag cgggtctgcc ctttggctta ttccactgaa ctgctccgaa
     1141 tagtatcttt gggtcatggg ataaatcatc actacagaat taaactcaag gcgacctaaa
     1201 aatctattcc gtttcaggag caattatcat gaataaaagc aaatccatcc tcgggctggt
     1261 gcttgggctt ttcctcgtcc tcgcttgtgg cgctcccgcc gccacgcccg atgtagccgc
     1321 tatagtgacc caaaccttgc aagccctcac ggctatcccc actgaggcgc aacaacaagc
     1381 cgaggttttg ccaactgctg aatcatccca acaactcaca cccaatggta cccccgtcga
     1441 caccgcagaa atttctttct tcattccaaa tggcgttgct aacgatgcca gctctaccat
     1501 gaccgcaaat gtggaatatc cctacatcaa cccatcttta ggcgatatgc cccagcatgc
     1561 ttccatcacc ctaaatttgt acgctgtcac aaaaagcatc cacgaagcac aaattttaat
     1621 cttccgtgcc tcagaatatt cacaatacag cgaatcaaat gcagcgacca tcagtcagct
     1681 ac
//
LOCUS       DAOQJE010000855         1680 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_64898_length_1680_cov_6.213538, whole genome shotgun sequence.
ACCESSION   DAOQJE010000855 DAOQJE010000000
VERSION     DAOQJE010000855.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1680)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1680)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1680
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_64898_length_1680_cov_6.213538"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(33..686)
                     /locus_tag="PLT08_17295"
     CDS             complement(33..686)
                     /locus_tag="PLT08_17295"
                     /inference="COORDINATES: protein motif:HMM:NF015956.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HNE06286.1"
                     /translation="MSVQPLSESTEMYLKAMVELGSRDVPISRLAERLSVTNVSANEM
                     MRRLAEQGLVTHTPYKGVTLTKKGKEVAVNVIRRQRLWECFLYDHLKIEWSKVYEFAC
                     SLEHATAPEVTEALAGFLNHPSTCPRGNPIPDEHGIFAPLKGRVISELKPGESATVLA
                     VSATDTEVLKYLQDKKILPGQELKFIEAAPFQGPLTVSVNGKEVALGLSLADYVIVK"
     gene            complement(831..1421)
                     /locus_tag="PLT08_17300"
     CDS             complement(831..1421)
                     /locus_tag="PLT08_17300"
                     /inference="COORDINATES: protein motif:HMM:NF020123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HNE06287.1"
                     /translation="MKKTQRYQKQKQQDQVKLSAIVGGGLVLLGFALFLVFQKPYSAS
                     ASETTIPDSVVPMAVEFPAPNLVLENVKSGTEALTDYHGKVVLVNNWATWCPPCKAEM
                     PSLQKFYEAHSGEGFMIVAVNAGDDRAPVEQFVKEYGLTFRVWLDPDGAALDAFRNAN
                     LPSSYVVDRTGIIRYAWTGEISYEMLEKYITPVIQN"
     gene            complement(1430..1639)
                     /locus_tag="PLT08_17305"
                     /pseudo
     CDS             complement(1430..1639)
                     /locus_tag="PLT08_17305"
                     /inference="COORDINATES: protein motif:HMM:NF024590.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
BASE COUNT          446 a          422 c          363 g          449 t
ORIGIN      
        1 gggtcaggca tagggaatac tccaaaatag ttttatttca caatgacata atctgccaaa
       61 gacaaaccca gcgcaacttc ttttccattg acggataccg tcagcggacc ctgaaatggc
      121 gcggcttcaa taaacttaag ttcttgccct ggcagaattt ttttatcttg caaatatttg
      181 agaacttctg tatccgtagc gctgactgcc aatacggtag ccgattcacc cggtttcaat
      241 tcactaatta cacgcccctt taaaggagcg aaaataccat gttcatctgg aatgggattg
      301 ccacgcgggc acgttgaggg gtgatttaga aaaccagcca acgcttcggt cacttcgggt
      361 gcagtagcat gttcaaggga acacgcaaat tcatatactt ttgaccattc gatcttgaga
      421 tggtcgtaca aaaagcactc ccacagccgt tgacggcgaa ttacattgac agccacctct
      481 ttgccctttt tcgtcagggt gaccccttta tacggggtat gcgtaaccaa tccctgctcc
      541 gcgaggcgtc tcatcatttc attggctgat acattcgtca cactcaggcg ttctgcaagg
      601 cgagaaatag gaacatcgcg acttcccaac tcgaccatgg cttttaggta catttcggtg
      661 ctttcactca acggctgaac agacataagg ctaccctatt aagaatgtta tggttcacct
      721 tattattcca aagagaccgt ttttccgtca tggaaagagg tcttaaattc cgtggataca
      781 tgtcatacat aaaaaaacgc cccgaatggg gcgttttttg tttattagaa ctaattttga
      841 ataaccggag taatatattt ttcaagcatc tcgtagctga tctcacccgt ccatgcatag
      901 cggataatcc ctgtacggtc caccacatag gagcttggca aattcgcatt gcggaaagca
      961 tccaacgcag ctccatctgg gtccaaccac acacgaaatg tcagcccata ttctttcaca
     1021 aattgctcca cgggagctcg atcatcccca gcgttgacag caacaatcat aaaaccttcg
     1081 ccagaatgtg cttcataaaa tttctgaagc gagggcattt ctgctttaca aggcgggcac
     1141 catgttgccc aattgtttac caaaaccact ttcccgtgat aatcagtcaa tgcctcagtt
     1201 ccactcttaa cattttcaag aacaaggttt ggagcaggaa attcaactgc catcggcact
     1261 actgagtcag ggattgtggt ctcagatgcc gaggcagagt atggcttttg gaaaacgaga
     1321 aacaatgcaa aacctagcaa aaccagcccg ccgcctacaa tagccgagag cttaacttga
     1381 tcttgttgtt tttgcttttg gtatctctga gtctttttca ttacaaagcc taagtttgca
     1441 ttgcgcgttt gatttcagcg accatctgag cttcgccata cgctccaacc acaatgccct
     1501 ttccggaatc gatcacagtt tgcgggactc cctgaacgtg gaactggtct gaaagttctg
     1561 ggaattccat ggcatcaatc gcctcagctc ggaccatggt tgggttttcc atagccattt
     1621 ggtgtgcgag caacacggct cgcgggcagt gcggtcaggt aggggtaacg aagacttgca
//
LOCUS       DAOQJE010000856         1678 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_65058_length_1678_cov_8.610598, whole genome shotgun sequence.
ACCESSION   DAOQJE010000856 DAOQJE010000000
VERSION     DAOQJE010000856.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1678)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1678)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1678
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_65058_length_1678_cov_8.610598"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..667)
                     /locus_tag="PLT08_17310"
     CDS             complement(<1..667)
                     /locus_tag="PLT08_17310"
                     /inference="COORDINATES: protein motif:HMM:NF023495.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rnase Y domain-containing protein"
                     /protein_id="HNE06288.1"
                     /translation="MNPIIIILIDILALLVGAVAGYFFHRYQVDRAAKARQERADDIL
                     KAAQTQANMIVKGAQDNATKIIQAAESELKERRIELNRDTERLEKRRSEVDSRYDKIE
                     QREQNLNRRQSQIDKRHNEAEKLFEDQMKKLEEIAQMTQDEARKDLFAAVEKEARGDM
                     ARIIRQIEAEAREEGEKRARKLIADAIQRVASEHVAEVTRAVVTLPSEEMKGRIVGRN
                     GRNI"
     gene            complement(670..1317)
                     /locus_tag="PLT08_17315"
     CDS             complement(670..1317)
                     /locus_tag="PLT08_17315"
                     /inference="COORDINATES: protein motif:HMM:NF014668.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RecX family transcriptional regulator"
                     /protein_id="HNE06289.1"
                     /translation="MRRITALEVQRKNPNRVNIYLDGEFAFGLARITAAWLKVGDILT
                     DEKITALQNEDARERALQQALLFLSYRARSEKEIRQNLKKHEYPEDAIEYTMTRLREN
                     RLANDNEFAQAWVENRNTFRPRSRRALTMELRQKGLDEETVKNAVADIDENALAYETA
                     RKRAPRLKSLEWSEFRKKLSEYLARRGFPYSVVSSTVTRIWNEAHTEETKYEDEE"
BASE COUNT          342 a          407 c          428 g          501 t
ORIGIN      
        1 tgatgttgcg tccgttacga ccaacgatac gtcccttcat ctcttcgctg ggcagagtga
       61 ccacggcgcg agtgacttcc gccacatgtt cagatgccac acgttgaatg gcgtcggcga
      121 tgagcttgcg ggcgcgcttt tcgccttctt cccgagcttc ggcttcgatc tgacggatga
      181 tgcgagccat gtcgccacgg gcttcttttt caacagcagc gaagaggtct ttgcgcgctt
      241 cgtcttgagt catctgcgcg atctcttcga gctttttcat ttgatcttcg aacaatttct
      301 cggcttcgtt atggcgcttg tcaatttgtg attgacggcg attgagattt tgttcgcgtt
      361 gttcgatctt gtcgtagcgg ctgtccactt cagagcggcg tttttcgagg cgctcggtat
      421 cgcggttcaa ttcaatacgg cgttccttga gctcactttc agcagcctga atgatcttgg
      481 tggcattatc ttgcgcacct ttaacgatca tattcgcctg ggtttgtgca gctttcaaaa
      541 tatcatcagc acgttcctgg cgtgctttgg cagcacggtc tacttgatag cgatggaaga
      601 aataccccgc tacggcacca actaaaagtg ccagaatgtc tatcaaaatt atgatgatcg
      661 gattcatgtt cattcctcat cttcgtattt tgtttcctct gtgtgcgcct cgttccaaat
      721 acgggtgacg gtagacgaaa ccacagagta ggggaagccg cgccttgcaa gatattcgga
      781 tagtttctta cgaaactcgc tccattccag acttttgagt ctgggtgccc gttttcgggc
      841 tgtttcatac gccagggcat tttcgtctat atcggcaacg gcgttcttga ccgtttcctc
      901 atcgagccct ttttgacgta gttccattgt cagtgcgcgg cggctgcgcg ggcggaatgt
      961 gttgcgattt tcgacccacg cctgagcaaa ttcattgtca ttggctaacc tgttctctcg
     1021 cagacgggtc atcgtatatt cgatggcatc ttcgggatat tcatgctttt tcaggttttg
     1081 ccttatttcc ttctcggaac gagcacgata actaaggaac aagagtgctt gttgcaaagc
     1141 tcgttcgcgc gcatcttcat tctgtaaggc ggtgattttc tcgtcggtga gaatatcgcc
     1201 cactttgagc catgcggctg taatgcgtgc caacccgaag gcgaattccc cgtcgaggta
     1261 aatgtttacc cgattggggt tcttccgttg tacttcgaga gcggttatcc ttcgcatttt
     1321 gttttttttg cgttttgctc cccctaaaac ttaaaaaggg gaggaattaa acgcgcacag
     1381 ccgaggcttc cgacagaatc ggtctcggct gtgaaaaggt ctggggttgg gtaccaggct
     1441 ctgggtccaa aaaagaggcg ttcccagaga taggtcgaaa tccgtggcgg gactccgtca
     1501 cgttcggcac ttgtagattt taaggtgaag ccagttggct caagttcgta tttctattgt
     1561 tggacttcac aaattaactc ccaaaattgg gggaggatcg tgcgcgaatt ttccagatag
     1621 tttcaagcaa aagaccgcaa agcggaaggt gtcttaagtt ttcttaatta tacatgaa
//
LOCUS       DAOQJE010000857         1678 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_65061_length_1678_cov_5.311768, whole genome shotgun sequence.
ACCESSION   DAOQJE010000857 DAOQJE010000000
VERSION     DAOQJE010000857.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1678)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1678)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1678
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_65061_length_1678_cov_5.311768"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(134..385)
                     /locus_tag="PLT08_17320"
     CDS             complement(134..385)
                     /locus_tag="PLT08_17320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HNE06290.1"
                     /translation="MLTIKVLGSGCANCKKVEAIAHQVVDQMGIQAEIIKVIEYPDIM
                     AYDIMSTPGLVINEKVVSYGRIPSPAEVTTWITNALQTA"
     gene            complement(397..1443)
                     /locus_tag="PLT08_17325"
     CDS             complement(397..1443)
                     /locus_tag="PLT08_17325"
                     /inference="COORDINATES: protein motif:HMM:NF015715.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HNE06291.1"
                     /translation="MTTLITPSVPHSDKKIFVSLAAALVLWLAAYNLIQPIANWISFD
                     LLKLSPDSQLGESLAFFLYDVPKILLLLSGMVFLISIVRTFFSPERTRALLGGKREGV
                     GNIFAALLGIVTPFCSCSAVPLFIGFVEVGIPLGVTFSFLIAAPTINEVAIILLFGLF
                     GWKVAGLYVASGLVVAIVAGFIIGRLKMEKYVADFVWKVHRKVNVTEEKLTWSDRIER
                     AWQSVKEIVGKVWLYVVVGIAVGAGIHGYVPEDALASILGAQAWWSVPLAVLIGIPLY
                     SNAAGIVPIVGTLIEKGASVGTALAFMMSVIGLSLPEAVILKRVLKMPLLVTFFVVIA
                     IAIIFTGYLFNLIL"
     gene            complement(1446..>1678)
                     /locus_tag="PLT08_17330"
     CDS             complement(1446..>1678)
                     /locus_tag="PLT08_17330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE06292.1"
                     /translation="SQHLMALRKADILQDRREGRYVFYKIKNASLLDLITASASLSGL
                     SAESVSSLINTQVYPSCECPHCVPALIPIASL"
BASE COUNT          488 a          426 c          399 g          365 t
ORIGIN      
        1 ctgccatttg cgaacggcag gaatttccgg tgcagaggaa taaaactttt ttcatggtct
       61 tctctcctta ttaatggtgt tcaatctttt accataagga gaactcttga attgttatgg
      121 tttggttaat ggctcatgct gtttgcaacg cgttggtgat ccatgtggtc acctccgcag
      181 gcgaagggat gcgtccatac gaaacgacct tttcattgat gaccagcccc ggtgtggaca
      241 tgatgtcata cgccatgatg tcgggatatt caatcacttt gataatctcc gcttgaatcc
      301 ccatctggtc aacgacttga tgagcaatgg cttccacttt tttgcaattg gcacaccctg
      361 agcctagaac tttgatggtt aacatttcat tttcctttac aagatcaaat tgaataaata
      421 gccagtgaag atgatcgcaa tggcgatcac gacaaagaac gtgaccaaaa gcggcatctt
      481 caaaacacgt ttgagaatca ccgcttcggg caaacttaag ccgatgacag acatcatgaa
      541 tgccagagct gttccaactg aagcgccttt ctcgatcaac gttccaacga tgggaacgat
      601 ccctgccgca ttggaataca acggaatacc gatcaacacc gccaacggaa ccgaccacca
      661 cgcctgcgcc cccaaaatag atgccaatgc atcttctggc acgtagccat gaatgcccgc
      721 ccccacggca atacccacca ccacatacaa ccagaccttg cccacgatct ctttcactga
      781 ttgccatgcg cgttcgatac gatcggacca ggtcagtttt tcttcggtca cattcacttt
      841 tcggtgaacc ttccagacaa aatctgccac atacttttcc attttgagtc gcccaataat
      901 gaagcctgcc acgatggcaa cgaccaaccc cgaagcgaca tacaaccctg ctaccttcca
      961 gccgaacaag ccaaacagca agatgatcgc cacttcattg atggtcggtg cggcgataag
     1021 gaaggagaaa gtcacaccaa gcggaatacc cacttcaaca aagccgatga aaagcggaac
     1081 agccgaacat gagcagaacg gggtgacaat tcccagcaga gcagcgaaaa tgtttcccac
     1141 accttctcgc ttgccgccca gcaaggcacg agtccgctcg gggctgaaga aggtgcgaac
     1201 gatagaaatg agaaagacca ttcccgacag taggagcaga atcttcggca catcgtagag
     1261 gaagaaagca agagactcgc caagctgtga atcaggggaa agcttcaaga gatcgaaaga
     1321 gatccaattt gcaatgggct gaataaggtt atatgcagcc agccatagaa caagggcggc
     1381 agccagagaa acaaatatct ttttatcaga atgcggaaca gatggggtaa tgagtgtggt
     1441 catggttaca aactcgctat gggaattaaa gcaggcacac aatgcggaca ttcacacgat
     1501 ggatagacct gcgtattgat gagggaggaa acagattcgg cggagagtcc gcttagggag
     1561 gcagaggcag taatcaggtc cagcagagag gcgttcttta tcttgtagaa aacataacgc
     1621 ccctcgcggc gatcctggag gatgtccgct ttgcggagcg ccatcaaatg ctgagaga
//
LOCUS       DAOQJE010000858         1677 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_65148_length_1677_cov_3.872996, whole genome shotgun sequence.
ACCESSION   DAOQJE010000858 DAOQJE010000000
VERSION     DAOQJE010000858.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1677)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1677)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1677
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_65148_length_1677_cov_3.872996"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..916
                     /locus_tag="PLT08_17335"
     CDS             <1..916
                     /locus_tag="PLT08_17335"
                     /inference="COORDINATES: protein motif:HMM:NF012602.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HNE06293.1"
                     /translation="ETHEGFEGKLDGLKTADTLLCVGANVNRSHMVAGFLFKRNLNAG
                     VNLINIDPDETSLDEIARLALKPKAGSDHQLILALQAVIAKEELERKSLNIKDADARI
                     KKAVAETGISEADLTKAARMLAHSISPMIVFGKGVTAQRDEKLIEALYQLAVQVGAVD
                     GERSNILSLKGESNSQSASLMGLDQPYNPNGKKVTYVAVGDDYVAKRLIEKATKSSYL
                     VVQASYESKLTEQADVVLPVSIWAEQEGHYINLDGRVRKAEKAINAPENVRDNLDVLT
                     ELAKRMKLTLDVDWKKAIRERKSSVSLN"
     gene            924..1466
                     /locus_tag="PLT08_17340"
     CDS             924..1466
                     /locus_tag="PLT08_17340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP oxidoreductase"
                     /protein_id="HNE06294.1"
                     /translation="MTKPKIASDWICGCAGCHMSLLDMDERILKIVELADLRSTPITD
                     LKEPDASGVDVGVLEGGVNNSANEEVAHKMRERSKILVALGDCAVFGGVPALRNLCGV
                     EAALRRAYVEAESNEAGSQIPNDPELATMTQARAVHEVVPVDYNVPGCPPDPDVIFYV
                     LSELAQGRKPELKDELLHWH"
BASE COUNT          468 a          390 c          451 g          368 t
ORIGIN      
        1 tgaaacgcac gaaggctttg aaggtaaact cgatgggctg aagactgccg atacattgct
       61 gtgcgttggc gcaaacgtca accgcagcca catggtggcg ggatttttat tcaaacgcaa
      121 tttgaatgct ggcgtgaacc tgatcaacat cgaccccgat gaaacttcac tggatgaaat
      181 tgcccgcctc gcgcttaagc cgaaagctgg ttctgaccat caacttatcc ttgcgctgca
      241 agcggtcatt gcaaaagaag agctggaacg caagtccttg aatatcaaag atgccgatgc
      301 gcgcatcaag aaagccgtcg ctgaaaccgg catctccgaa gcggacttga ccaaagccgc
      361 gcggatgctg gcacattcca tctcgccgat gatcgtcttt ggtaagggcg tcaccgctca
      421 acgcgatgaa aaactgatcg aagcgctgta ccagcttgca gtccaagtag gagccgtgga
      481 tggcgaacgc agcaacatcc tttcgctgaa aggtgaatcc aatagccaat ccgcttcgtt
      541 gatggggctc gaccagccat acaatcccaa tggcaagaaa gttacttatg ttgcagttgg
      601 cgatgattat gtggcaaaaa gattaatcga aaaagctaca aagtcgtcct atctggttgt
      661 gcaggcaagt tatgaatcaa aactaactga acaagccgat gtggtgctgc cggttagtat
      721 ttgggcagag caggaaggtc attacatcaa cctcgatggt cgcgtacgca aagccgagaa
      781 ggcgatcaac gcgccagaga acgtgcgcga caatttggat gtgctgaccg aacttgctaa
      841 acgaatgaaa ttgacattgg atgtggattg gaagaaagcc atccgtgaac gcaaatctag
      901 cgtgtcgctg aattgaggat attatgacaa aaccaaaaat cgcttctgat tggatctgcg
      961 gctgcgctgg ctgccacatg tctttgctcg atatggacga acgcattttg aagatcgttg
     1021 aactggcgga tttacgctcc acccccatca cagatttgaa agaaccagat gccagtggcg
     1081 tggacgtggg tgtgctcgaa ggtggcgtga acaattctgc gaatgaagaa gtggcacaca
     1141 agatgcgcga gcgctcaaag atcttggtag cacttggtga ctgcgccgta tttggcggcg
     1201 tgcctgcact gcggaatctc tgcggtgtgg aagcggcgct gcgccgcgcc tacgtggaag
     1261 cggaaagcaa tgaagcagga agccaaattc ccaatgatcc cgaactagca accatgactc
     1321 aggcgcgcgc ggtgcatgaa gtggtgcccg ttgattacaa cgtgcccggc tgcccacccg
     1381 accccgatgt gattttttat gtgctttcag aactggcaca gggacgcaag ccggaattga
     1441 aagatgagtt attacattgg cattagaagt aaatgcagaa tgatgaatga ggaatagctt
     1501 atgacaacac aaaaaattac catagaacct gtcactcgca ttgaaggtca tgccaaggtg
     1561 acgatacaca tgaaagacga taataccgtt gaccatgcct atatgcacgt caacgagttt
     1621 cgtgggtttg aaaaattctg cgaagggcgg ttgtattttg aaatgccgca gatcaca
//
LOCUS       DAOQJE010000859         1677 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_65171_length_1677_cov_2.940197, whole genome shotgun sequence.
ACCESSION   DAOQJE010000859 DAOQJE010000000
VERSION     DAOQJE010000859.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1677)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1677)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1677
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_65171_length_1677_cov_2.940197"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..687)
                     /locus_tag="PLT08_17345"
     CDS             complement(<1..687)
                     /locus_tag="PLT08_17345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE06295.1"
                     /translation="MKAFGRFFAQLAFYTISIGLLVYAASRSLHFIQASLDEDNQILG
                     MFALLATSGGMIGWLTIFLFKADGIVQKGIALLMVLVDFLGEAALFTMDTLQQAGANG
                     VVKELTQSEARTVLIGMSILIALNILAGIAYHLGNMEIIRQMAEGAQKDILFFKSLAE
                     IEKNTDDLARTMAPSIASEWKTEFTKNYGTASKLGLTGGKSEEVPSTSLSSSTSSPIK
                     LRLPRFNILPW"
     gene            complement(690..1088)
                     /locus_tag="PLT08_17350"
     CDS             complement(690..1088)
                     /locus_tag="PLT08_17350"
                     /inference="COORDINATES: protein motif:HMM:NF019844.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0070064 - proline-rich region binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HNE06296.1"
                     /translation="MKIKPSKYPSIKYRSLALWAISLCVISLACSLTDSSTRPSVTPT
                     SSPIARDYAIPIEAKQTLDLSAISSQTSCTVSATVLHLRSCAGTNCPVDAWLLQGESL
                     TILQTGTTWINVQTSNGKTGWVNATYCGGK"
     gene            complement(1321..1563)
                     /locus_tag="PLT08_17355"
     CDS             complement(1321..1563)
                     /locus_tag="PLT08_17355"
                     /inference="COORDINATES: protein motif:HMM:NF024139.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HNE06297.1"
                     /translation="MTAGKIPSDEEAILSVEFLTVAQVARWARVSTKTVYRWIEAKKL
                     PAIKFGKRTYRIPTRAVIEYLKSAGYADLIALPKPK"
BASE COUNT          419 a          359 c          461 g          438 t
ORIGIN      
        1 ccacggcagg atgttgaagc gcggcaggcg cagtttgatg ggggaggatg tcgatgagga
       61 aagtgaagta gaaggtacct cctcactctt ccctccggtc agtccaagct tcgaagcagt
      121 cccgtaattt ttggtgaact cggttttcca ctcgctggcg atcgagggcg ccatggtgcg
      181 ggcgaggtcg tcggtgtttt tctcgatctc cgccaaggat ttgaagaaca gaatatcctt
      241 ctgagcgcct tccgccattt gacggataat ctccatgttg ccaaggtgat aggcgatgcc
      301 tgccagaata ttgagggcga tgaggatgga catgccgatc aatacagtcc gtgcctcgga
      361 ttgagttaac tctttcacaa ctccattcgc acctgcttgt tggagggtat ccatcgtaaa
      421 cagtgcggct tcaccgagaa aatccaccaa gaccataagc aaggcaatgc ccttttgaac
      481 aatcccatca gctttgaaca ggaagatggt tagccaaccg atcatgccgc cagatgttgc
      541 gagtaaggca aacatgccaa ggatttgatt atcttcatcc agcgacgctt gaatgaaatg
      601 aagcgagcga ctggctgcgt acaccagcag accgattgag atcgtataga acgcgagttg
      661 agcaaaaaag cggccaaagg ctttcatgat tatttacctc cacaataggt tgcgttgacc
      721 cagccggttt tcccgttgct ggtttggacg ttgatccagg ttgtgcctgt ttgaaggatg
      781 gtgagggatt cgccttgcag gagccacgca tctaccgggc agtttgtccc ggcgcaagag
      841 cgtaggtgga ggacagttgc actgacggtg caagaagttt gtgaagagat agctgacaga
      901 tcaagagtct gttttgcttc gatgggaatt gcatagtcac gtgcgatggg ggaggaggtg
      961 ggcgtcactg atggacgtgt ggagctatca gtcagcgaac atgcaaggga aattacgcag
     1021 agagatattg cccataaagc taatgaacga tattttatgg atgggtattt tgagggttta
     1081 attttcattg tgtctcctag ttgaaaaaat tcgttatgtt agaataggag acgcagatat
     1141 tttctgtatg ggcgctgttg taccggctag gtatgtaaac agcgcccgct cttatctgcg
     1201 tcatggtagg gtagtgggaa gattagccag catcacctcc ttgcaataga gatttatttt
     1261 gttcaaaatg aaagctcgaa atgaaataat gaagatgtga tgttattgat tcggcaatga
     1321 tcacttgggc ttaggcagag caataagatc tgcataccct gctgatttga gatactcaat
     1381 aactgcacga gtagggattc gataggtacg tttgccaaat ttgatggctg gtaacttctt
     1441 cgcttcgatc cagcgatata cagtttttgt gctaactcgt gcccatctag ctacctgagc
     1501 gaccgtgagg aattccacgc tgagtatggc ttcttcgtct gagggtattt ttccggctgt
     1561 catagaatct ccctggagcc ggtgaaaagg taaaacaaga aaccccggct ccgatgaatt
     1621 tcttcatcag aaaccggggt ttctttgcgc agagttacgc tctgcggtcc cgacggg
//
LOCUS       DAOQJE010000860         1659 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_66550_length_1659_cov_7.473192, whole genome shotgun sequence.
ACCESSION   DAOQJE010000860 DAOQJE010000000
VERSION     DAOQJE010000860.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1659)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1659)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1659
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_66550_length_1659_cov_7.473192"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..494
                     /locus_tag="PLT08_17360"
     CDS             <1..494
                     /locus_tag="PLT08_17360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE06298.1"
                     /translation="DGFTDQRIVSGKWTIENNQAVQTVTDVSDYVWNTGVQASEYTVS
                     AKITLPNTADSGGGFIIHMSERGSKNNSYIVRLKDGGKGIWWGSINDEGKFKGQGSAP
                     IEGTEKAFTLKLVVKLDTLSIYVNDVEIAKNVAISSQEGWVGLLAYGGKVTFEDVKLE
                     VEK"
     gene            491..1657
                     /locus_tag="PLT08_17365"
     CDS             491..1657
                     /locus_tag="PLT08_17365"
                     /inference="COORDINATES: protein motif:HMM:NF016104.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000009 - alpha-1,6-mannosyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0004376 - glycolipid mannosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006506 - GPI anchor biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyltransferase family protein"
                     /protein_id="HNE06299.1"
                     /translation="MKRLPDWLTIPFLAFLLSRTLIFGAGILGDTMLPTEEGHWTADE
                     NSPFLSMWAKWDSQYYVDIATNGYWFRPGQQSNVAFFPLYPMTMQVVAKFLGGNLILT
                     GFIISNLAFLGGLIFFYLLTELELGSDSAKRAVYYLAFFPTSFFFSSVYTESMFVFLS
                     IATMYFARKHQWMWAAVFGILTAATRNLGILMWALCIWEWMRSQGWAFLSMHKKQTWL
                     NLWNGFKQHWFDLVTISLIPLGMLVFIFFLKINFDRPLAFIEVQAAWGRENIGPLAVL
                     KKSITSLITGEINKGWLTQFWNVSFYLLFLMMVPFIWLKLGEGYAIFVLIMLFVPSAS
                     AIGSVIRYLLTQFPVYMMMAWWGRDDRVDRAFNFTFATLLGVFVVIFVNWIFVA"
BASE COUNT          383 a          370 c          409 g          497 t
ORIGIN      
        1 tggatggttt taccgatcag cgcattgtca gcggcaaatg gacgattgaa aataaccaag
       61 ctgttcaaac cgtaaccgat gtttcagact atgtttggaa tacgggcgta caggcttcag
      121 aatatacggt cagcgctaaa atcactttgc ccaacacagc agattcgggc ggcggtttca
      181 tcatccacat gagtgagcgt ggctcgaaaa ataattcata cattgtgcgc ctcaaagatg
      241 gtggcaaagg catttggtgg ggcagcatta acgatgaagg caaattcaag gggcaaggct
      301 ctgcgcctat tgagggaact gaaaaagcat tcacgctcaa gctggtagtc aaacttgata
      361 ccctcagtat ttatgtaaat gatgtagaga tcgcaaagaa tgttgccatc tcttcgcaag
      421 aaggttgggt gggtttgctc gcgtatggtg gtaaggtcac atttgaagat gtgaaacttg
      481 aggtggaaaa atgaaacgcc tgcccgattg gctcaccatt ccattcttgg cattcttgct
      541 tagccgcacg ctcatatttg gcgctggcat tctcggcgac acgatgttgc cgactgaaga
      601 aggtcactgg accgcagacg agaacagccc attcctttcg atgtgggcga agtgggatag
      661 tcaatattat gtagatatcg ccaccaacgg ttactggttc cgcccgggtc aacaaagcaa
      721 tgtagcattc tttccgcttt acccgatgac tatgcaagta gtggcgaaat tcctaggtgg
      781 caatttgatc ttgactggat tcattatcag caacctggca tttcttggcg gattgatctt
      841 tttctacttg ttgacggaac ttgaactcgg ctcagactcc gccaagcgtg cggtgtacta
      901 cctggcattt ttccccacct cgttcttttt ctcttcggtc tacaccgaga gtatgttcgt
      961 gtttctttcc attgctacta tgtactttgc ccgcaaacat cagtggatgt gggcggcggt
     1021 ctttggcatt ctcacggctg ccacacgcaa ccttggcatt ttgatgtggg cgctttgcat
     1081 ttgggagtgg atgcgttctc aaggttgggc attcctctcc atgcataaaa aacagacttg
     1141 gctcaattta tggaacggct tcaaacagca ttggttcgat cttgtcacca tctcgctgat
     1201 tccgttgggc atgttggtct tcattttctt cctcaaaatc aacttcgacc gtccactggc
     1261 tttcattgaa gtgcaagcag catgggggcg cgagaatatt ggtccactgg cggttttgaa
     1321 gaaaagcatt acttctttaa taactggcga aattaacaaa ggctggctta ctcagttctg
     1381 gaatgtttct ttttatctgc ttttcttaat gatggttccc ttcatttggc taaaactcgg
     1441 tgagggatat gccatcttcg ttctcatcat gctgttcgtc ccatctgcca gcgctattgg
     1501 aagtgtcatc cgctatttgc tgacgcaatt ccctgtgtac atgatgatgg catggtgggg
     1561 gcgtgatgac cgtgttgacc gtgcttttaa ctttaccttc gccaccttgt tgggtgtgtt
     1621 cgtagtgatt tttgtcaatt ggatttttgt tgcataaaa
//
LOCUS       DAOQJE010000861         1656 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_66794_length_1656_cov_4.143036, whole genome shotgun sequence.
ACCESSION   DAOQJE010000861 DAOQJE010000000
VERSION     DAOQJE010000861.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1656)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1656)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1656
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_66794_length_1656_cov_4.143036"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(70..1239)
                     /locus_tag="PLT08_17370"
     CDS             complement(70..1239)
                     /locus_tag="PLT08_17370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE06300.1"
                     /translation="MAYIVDGNLYIQNGSNPPTQLTDNRQDIYPMFSDDGEKIVFYRG
                     RNNENNSIFSINADGSHIQEIITKGWLDTLGVGTKAGHLAFIPNTHQFLFNTYLCPDN
                     DDMYSGCTVGLFLADTNTGKLKEIVKPALGGRLLLDGDSRWGGNFSISPDGNLLSIAH
                     AGQIDILSMDGKVIHHNIMEYPPGMPIELYPRVYWFPDSSGFITALPAEKEYYFWVMS
                     GNPAYTIWRYTFDKNIATQIPLDPTLLWIRMDSNDVVSVSPNREWVVYFANDCQLYKG
                     NLLDGSTELLLPCKYHLEIQWNLDNMHFASGAYPEESIFGSIDSPLGYPPGYFLGWID
                     AKRFLYFPASARSKENIQLLVGEIDGEIILSYESNVFIPNKAPYPPIFFVYSFGE"
BASE COUNT          452 a          336 c          377 g          491 t
ORIGIN      
        1 aaatttgtac gcaggtttgc tcgcttcgcc ggggacggcg gcttgagcgg gggaaaccat
       61 ctcttttatt tattctccaa aagagtaaac aaaaaagata ggggggtatg gcgctttatt
      121 tggaataaaa acatttgact cgtaagacaa aattatttcc ccgtcaattt cacctacgag
      181 aagttgtata ttttctttac ttcttgccga ggcggggaag taaagaaatc gttttgcgtc
      241 aatccaaccc aaaaaatatc ctggaggata acctagcgga gagtcaatag agccaaaaat
      301 agattcctct ggatatgcac cactagcaaa atgcatattg tccaaattcc actgaatttc
      361 caaatgatat ttgcatggta ggagtaattc tgtacttcca tctaaaagat tacctttata
      421 tagttggcag tcatttgcaa agtaaacaac ccattccctg tttggtgaga cgctcactac
      481 atcattagaa tccatgcgta tccataacag agttggatcc aaaggtattt gcgttgctat
      541 gtttttatcg aatgtgtatc tccaaatggt gtaggcggga tttccagaca tcacccaaaa
      601 ataatattct ttttctgctg ggagggcggt gataaacccg cttgaatctg gaaaccagta
      661 cactcttggg taaagttcaa ttggcattcc aggaggatat tccatgatgt tgtggtgaat
      721 aaccttgcca tccatgctga gtatatcgat ttgtcctgca tgagctatgg ataataaatt
      781 cccgtcaggg gatatactga aattgcctcc ccaccgcgaa tcgccatcca aaagcaaacg
      841 accgccaagg gcgggcttca caatctcttt gagttttccc gtattggtat ccgcaaggaa
      901 tagtcccact gtacagcctg aatacatatc gtcattatca ggacacaagt atgtattaaa
      961 aaggaattga tgcgtgttgg ggataaacgc cagatgtccc gcttttgtgc ctacgccgag
     1021 agtatccaac caaccttttg tgatgatttc ttgtatatga cttccatcgg cgttgatcga
     1081 aaatatactg ttattttcat tattcctgcc acgataaaag acaatcttct cgccatcatc
     1141 ggagaacatg ggataaatat cttgtctgtt atccgttagt tgtgtgggtg ggttactgcc
     1201 attttgaata tacaaatttc catccacaat atatgccatg cggagattat ccagcgaata
     1261 gatttcagtc gctgtggata ctggtatcgg ggcaggagat gggattgttg ttggatcaat
     1321 gattgtgact ggattggggg taagcgtcgg cagcggggac aaggtctctg tgggagggat
     1381 ttgtgccaca gtcgcctgcg gggtgaaagt cacattaggc gagcaactga ccagtacgca
     1441 ggtcaacagg gcggcgagta gttttttttt cataaggtat cctttttggg gaggtgtgtc
     1501 gtttcagttc ccccaccaga atataaatta tagcgttatc tgatcattcc ttcctggctg
     1561 cttatcgcac aaaataagaa tcctccgagt ttttcagcac acaaaactcg gaggattctt
     1621 gattcgtttt attttcggct gatggtttat tcagag
//
LOCUS       DAOQJE010000862         1649 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_67337_length_1649_cov_7.773526, whole genome shotgun sequence.
ACCESSION   DAOQJE010000862 DAOQJE010000000
VERSION     DAOQJE010000862.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1649)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1649)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1649
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_67337_length_1649_cov_7.773526"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..234
                     /locus_tag="PLT08_17375"
     CDS             <1..234
                     /locus_tag="PLT08_17375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE06301.1"
                     /translation="EPLLEYIADLAAKRQPGEIITVVVPEFVSENRWTAALHTNTADI
                     LRNQLKNQHGIVITNVPYHVHEAEGDHADHVAH"
     gene            247..912
                     /locus_tag="PLT08_17380"
     CDS             247..912
                     /locus_tag="PLT08_17380"
                     /inference="COORDINATES: protein motif:HMM:NF014325.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HNE06302.1"
                     /translation="MNFIVIGCGRFGAELAYKLFQNGHQVVVVDANPKAFNRLHPDFR
                     GRTVEGDSLSADTLSRASADKATGIAVVTNSDTMNAVIGHTIRVHYPNVEQVIVRNYD
                     PAMRDMLEAFGLQIVSSTAWGAERVQELLIDPSFRAVFSAGNGEVEMYEMYISPKWDG
                     MTISNLLDGCGEIVCSALTRAGRAQLPNGGMKLQKGDILTVSATLEGVKALRAKLQEG
                     KEA"
     gene            914..1594
                     /locus_tag="PLT08_17385"
     CDS             914..1594
                     /locus_tag="PLT08_17385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-binding protein"
                     /protein_id="HNE06303.1"
                     /translation="MLVLIAGGGRTGAQLAAQLLQQNYEVRLVEHRKELLARLHHELP
                     TEVIYEGQATDPNVLRQAGLEKANVIVACTNDDAANLVLCYLARTLFKVRRTVARINN
                     PRNSWLFDNNFHVDETINQADVMAHLIQEEMSLGDMMTLLKIRRGRYSLVEEKVPKGA
                     KAIGMQLKDLGLPEQCVIAAIIRDGQVTLPRGTTELEEFDEVLAITDDEGAKQLAHLL
                     EPPDRSVL"
BASE COUNT          411 a          413 c          420 g          405 t
ORIGIN      
        1 gagccgttgc ttgaatacat tgcagacctc gcagcgaaac gtcaacccgg agagatcatc
       61 acagtggtgg tgcccgagtt cgtttccgag aatcgttgga cggctgccct gcacacgaat
      121 acagcagaca ttctgcgtaa tcaattaaag aatcaacatg gcattgtgat taccaatgtg
      181 ccttatcatg ttcatgaggc agagggcgac catgccgatc atgttgccca ctaaggagtt
      241 tttactatga atttcattgt tattggatgt ggaagatttg gcgctgaact ggcgtataaa
      301 cttttccaaa atggacatca agttgtggtg gtggatgcca accccaaggc gttcaatcgc
      361 ctccacccag actttcgcgg gcgtaccgta gaaggtgact ctctctccgc tgacacctta
      421 tcccgcgcca gcgccgataa ggcgaccggc atcgctgtgg tcaccaattc agacacaatg
      481 aacgccgtta tcggacatac cattcgggtg cattacccga atgttgaaca agtcatcgtc
      541 cgcaattatg accccgccat gcgcgatatg ctcgaagcct ttggactgca aatcgtcagc
      601 tccacagcat ggggtgcgga gcgtgttcag gaactcttga ttgacccatc tttccgtgcg
      661 gtattctctg ccgggaatgg cgaagtagaa atgtatgaga tgtacatctc cccgaaatgg
      721 gacggcatga ccatctccaa cttgctggat ggttgtggcg agattgtctg ctctgcattg
      781 actcgcgctg gacgcgctca attgccgaat ggcggaatga aattacagaa gggtgatatt
      841 ctcaccgtaa gcgcgacctt ggaaggcgtg aaagctttac gtgcaaaatt gcaggaaggc
      901 aaggaggcat aacatgcttg tacttattgc tggcggcggg cgcactggcg ctcaactggc
      961 tgctcaactt ctccaacaaa attacgaggt gcgcctcgta gaacatcgca aggaattgct
     1021 ggctcgcttg catcatgaac ttcctaccga agttatctat gaaggtcagg cgacagaccc
     1081 gaacgtgctc cgtcaagctg gtttggaaaa agccaatgtg atcgtggctt gtaccaacga
     1141 tgatgctgca aatttggtct tgtgctatct ggcgcgtacc ctctttaaag ttcgccgcac
     1201 agtggctcgt atcaacaatc cgcgcaatag ctggctcttt gataataact tccacgtgga
     1261 tgaaaccatc aaccaagcgg acgttatggc tcacctcatt caagaagaaa tgtcactcgg
     1321 cgatatgatg accctgctca agatccgccg tggacgctac tcattggttg aggagaaggt
     1381 gcccaagggt gccaaagcca ttggtatgca gctcaaagac ttgggtttgc ctgagcaatg
     1441 tgtcatcgct gccatcatcc gtgatggaca ggtgaccctt ccgcgcggta ctaccgagct
     1501 tgaagaattt gacgaagtgc ttgcgattac cgatgatgag ggcgcaaaac aattagccca
     1561 cctcttggaa ccacctgatc gttctgtgtt gtaaaacaaa aacctcccga aacaattcgg
     1621 gaggtttttt cttattttga aaccatttc
//
LOCUS       DAOQJE010000863         1645 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_67659_length_1645_cov_7.137736, whole genome shotgun sequence.
ACCESSION   DAOQJE010000863 DAOQJE010000000
VERSION     DAOQJE010000863.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1645)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1645)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1645
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_67659_length_1645_cov_7.137736"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..381
                     /locus_tag="PLT08_17390"
     CDS             <1..381
                     /locus_tag="PLT08_17390"
                     /inference="COORDINATES: protein motif:HMM:NF012766.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M24 family metallopeptidase"
                     /protein_id="HNE06304.1"
                     /translation="AVGEMDAECQKIHKLVQEANAAGRAIAKPGIPCADVDKATRDVI
                     EKAGYGKYFNHRTGHGIGMEGHEAPYMRGDNMQILEPGMAFTVEPGIYLTNRNGVRIE
                     DNMVITENGAESLSDMPREIRTVG"
     gene            complement(469..1149)
                     /locus_tag="PLT08_17395"
     CDS             complement(469..1149)
                     /locus_tag="PLT08_17395"
                     /inference="COORDINATES: protein motif:HMM:NF025687.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4386 domain-containing protein"
                     /protein_id="HNE06305.1"
                     /translation="MNSLNKTSKLAGVLYLIITFIAPFSMIYVPSKLMVAGDALATAN
                     HIESAQGLFRAAIASDALVFLLEIALTVLVFQLVKSVDKTLALISAYARLGMTIIQGV
                     NVINYLFVLLLVNGAEYLSALANGQSQALVSLFLHAHDSVALIWGLFFGIHLLTLGYL
                     VYKSGYMPKWLGVVLLITSLLYLAQGFGIILFPQIEGQMKSLMSFAFIEIAFPLWLLI
                     KGVKDQPV"
     gene            complement(1164..>1645)
                     /locus_tag="PLT08_17400"
     CDS             complement(1164..>1645)
                     /locus_tag="PLT08_17400"
                     /inference="COORDINATES: protein motif:HMM:NF024989.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="NAD(P)-dependent alcohol dehydrogenase"
                     /protein_id="HNE06306.1"
                     /translation="GTFAVQIAKAFGAEVTAVCSTRNVELARSLGADHVIDYTKEDFT
                     KSGKQYDLILAANGYHSLAEYQRALTANGKYVMTGGTTAQMFEVLLFGRMKSKGNQKL
                     GNILAKPSQKDLEFVAGLLADGKVKPVIAKRFALAHTADAIRYVEEGHAQGKVVVTI"
BASE COUNT          454 a          365 c          405 g          421 t
ORIGIN      
        1 gcagtggggg agatggatgc tgaatgtcag aagattcata aacttgtaca agaagcgaac
       61 gccgctggtc gtgcgattgc caaaccgggt atcccttgtg ctgatgtaga taaggcaacc
      121 cgtgatgtca ttgaaaaagc cggttatgga aaatatttta accaccgcac gggtcacggc
      181 attggtatgg aaggacacga agcgccatac atgcgcggtg acaatatgca aattttagag
      241 ccgggcatgg cgttcacggt tgagccggga atttatctca ccaatcgcaa cggcgtacgc
      301 attgaagata acatggtcat tactgagaat ggtgccgaaa gcctctctga tatgccaaga
      361 gaaatacgaa cggttggtta acatagaagc cgcgaatttt ataaattcgc ggcttctttt
      421 cttaaattca gcaggatcga aacgtaccaa aatcagatgg ataactgttt acaccggttg
      481 gtcttttacc ccttttatta ataaccataa tgggaatgca atttcgataa aggcgaatga
      541 catcagagat ttcatttgtc cctcaatttg cgggaagagg atgatgccga aaccttgcgc
      601 aagatatagc agggaagtga ttagcaatac aactcccagc cacttaggca tataacctga
      661 tttgtacacc aagtatccta gcgtcagtaa gtgaataccg aagaaaagtc cccagatcag
      721 ggcgacagag tcatgggcat gtagaaatag cgaaacaagc gcttgcgact gtccatttgc
      781 aagagcggat agatactctg ctccattcac cagtagtaag acgaagagat agttaatcac
      841 attgacacct tggatgattg tcatacctaa gcgggcatat gcagagatca gcgcgagagt
      901 cttgtccaca gatttgacga gttgaaaaac tagtacggtc aaggcaatct cgagcagaaa
      961 gaccaaagcg tcacttgcaa tggcggcgcg gaagagccct tgggcagatt caatatggtt
     1021 tgctgtagca agtgcatcac ctgccaccat caatttagaa gggacgtaaa tcatgctgaa
     1081 tggggcaatg aaggtgatga tgagatacag taccccggca agttttgaag ttttattgag
     1141 tgagttcatt gtgttctcct tttctatatc gtgacaacaa ctttgccctg cgcgtgaccc
     1201 tcttccacgt agcggatggc gtctgcggta tgggcaagtg caaagcgttt tgcaataacc
     1261 ggtttcactt tgccgtctgc caacaagcct gctacaaact ccaaatcttt ttggctgggt
     1321 tttgccaaaa tgttgcccag cttttgattg cccttcgatt tcatacgccc gaatagcagc
     1381 acttcaaaca tttgggcagt tgtaccgcct gtcatcacat atttcccatt tgccgttaga
     1441 gcgcgttgat attcggcaag ggagtgatat ccgtttgcag ctaggatcaa gtcatattgc
     1501 ttgccgcttt tggtgaaatc ttctttggtg taatcaatga catggtctgc accaagtgag
     1561 cgtgccaatt ccacattacg cgtgctgcac accgcggtga cctctgcccc gaaggctttg
     1621 gcaatttgca cggcgaaggt cccta
//
LOCUS       DAOQJE010000864         1644 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_67756_length_1644_cov_4.344871, whole genome shotgun sequence.
ACCESSION   DAOQJE010000864 DAOQJE010000000
VERSION     DAOQJE010000864.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1644)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1644)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1644
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_67756_length_1644_cov_4.344871"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1528
                     /locus_tag="PLT08_17405"
     CDS             <1..1528
                     /locus_tag="PLT08_17405"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HNE06307.1"
                     /translation="MLMLATVRIGAIHSVVFAGFGEKALGDRIQASGSRLVFTTDITY
                     RKGKDVNLKSIVDGALAQVDNSVEHLVVLKRGSQDVPMNTPLDITWEEFLEHSKGQPS
                     DCVPMEANEPAFILATSGTTAKPKLAIHTHGGYQVHIASMARWVFGLKPEDVWWATSD
                     IGWIVGHSYIVYAPLIIGCTTIAFEGALDHPSSETNWKIAIEEFSVSGVFTSPTAVRL
                     LMRYGDEPLGRVNHDRVERIFCAGEVLNAPAWDWLQNKMLKNRIPVIDHMWQTETGGP
                     VFGNPYGLGMLPIKPGSATIPLPGIQVEVMNMDGQPCAPNEKGIMVIKRPFPGLTPAL
                     WGEPERYGNDYWNKIPNVYYTGDSAHMDADGYVWFAGRADEIIKIAGHRIGTIEVETA
                     FLKHPAVAESGVIGRPDEIRGEVISAFVLLKNGFQASEALKKELLETVRHELGPVAVI
                     GELNFVNMLPKTRSGKIMRRVLKAVTLDKDPGDITTIEDEGSVTEAREAWQEMKNALS
                     "
BASE COUNT          437 a          388 c          423 g          396 t
ORIGIN      
        1 tatgctgatg ctagcaaccg tccgcattgg tgcaattcac tcggtagtgt ttgcaggttt
       61 tggcgaaaaa gcgttaggtg accggattca agccagcggc tcgagattgg ttttcacgac
      121 agatatcaca taccgcaagg ggaaagatgt taatctaaaa agtatcgtcg acggcgccct
      181 ggcacaagta gataacagcg tggaacatct tgtggtcttg aaacgcggtt ctcaagatgt
      241 acctatgaat actccgctcg atattacttg ggaagaattc cttgaacact ccaagggtca
      301 acctagcgat tgtgttccga tggaagccaa tgaaccggct ttcatccttg ctacttctgg
      361 taccactgcc aaacccaaac tggcaatcca tacccatgga ggttatcaag ttcacattgc
      421 cagcatggca cggtgggtct ttggattaaa gcccgaagat gtatggtggg caacctctga
      481 tattggttgg atcgtgggac acagttatat tgtctatgcg ccgctcatca tcggctgtac
      541 gaccatcgcc tttgaagggg cgctcgatca tccaagttct gaaacgaatt ggaagatcgc
      601 cattgaagaa ttttcagtta gcggggtttt cacatctcca actgctgtcc gtttattgat
      661 gcgatatgga gacgaaccgc taggtcgtgt caatcacgat cgcgtagaac gcatcttttg
      721 cgcaggcgaa gtcctcaacg cacctgcctg ggattggttg cagaacaaga tgttgaagaa
      781 tcgcatccca gttattgacc atatgtggca aaccgaaacg ggtggacctg tttttgggaa
      841 cccctacggc ttagggatgt tgcccatcaa acctggctcg gcgaccattc ccttacccgg
      901 cattcaggtt gaggtgatga atatggacgg tcagccatgt gctccaaacg aaaaaggcat
      961 catggtcatc aaacgcccct tccctgggtt gactcctgct ttatggggcg agccagaacg
     1021 atatggaaac gattattgga acaagattcc caacgtctac tacactggtg actcggctca
     1081 tatggatgca gatggttacg tgtggtttgc agggcgggca gatgaaatca tcaagattgc
     1141 tgggcatcgc attggcacca tcgaggtaga aacggcattt ctcaagcacc cagccgtagc
     1201 agagtcgggc gtcatcggca gacccgatga gattcgtgga gaggtcatct cagcctttgt
     1261 attgctcaag aatgggttcc aagcttctga ggcgctgaaa aaagaattgc ttgaaaccgt
     1321 ccgccacgag cttggacctg tggcagttat cggcgagtta aacttcgtca atatgttgcc
     1381 caaaacccgc tccggcaaga tcatgaggcg tgtactaaaa gcagtgaccc tcgataaaga
     1441 ccccggtgac atcaccacga ttgaagatga aggctccgtc actgaagctc gtgaagcctg
     1501 gcaagaaatg aaaaacgccc tatcttaaat gagtaacact cttacgcaat tttagaaaag
     1561 cggattaaaa tacccgctaa catccacata tcacttttgg aggctggcat ggcgaagaag
     1621 aaggaaatgg aaggcgaagt ctat
//
LOCUS       DAOQJE010000865         1643 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_67827_length_1643_cov_3.442065, whole genome shotgun sequence.
ACCESSION   DAOQJE010000865 DAOQJE010000000
VERSION     DAOQJE010000865.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1643)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1643)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1643
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_67827_length_1643_cov_3.442065"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1098)
                     /locus_tag="PLT08_17410"
     CDS             complement(<1..1098)
                     /locus_tag="PLT08_17410"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014442.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent DNA methyltransferase"
                     /protein_id="HNE06308.1"
                     /translation="MPNNFSEKANFIWQVADDILRGAFHAYEYGSVILPFVVLRRLDQ
                     VLEAQKDAVIKAHDQFKDSLDEERIIPVLKQAAGGTNFYNHSFYDLRRLAQDSKNIEI
                     NFSNYLNGYSKNVREIIENFQLEKIITKLIKNNLLYLLVEKFAAVDLSPASVTNHEMG
                     YIFEELLRRFSEMSNETAGEHYTPREVIRLMVSLMFAEHKDELKGKGIIRSVFDPACG
                     TGGMLITAKEYIQEHINADVEVVMYGQELNEQTYAIAKSDVLIMGENAENIRQGSSFK
                     NDRFKGQQFTYMLSNPPFGVSWKMEEDFIKNEANDPHGRFHVGTPRTSDGALLFLQHM
                     ISKMEANGSRIAIIFNGSPLFTGDAGSGESDI"
BASE COUNT          401 a          393 c          411 g          438 t
ORIGIN      
        1 gatgtcgctc tcgcctgaac ccgcgtcccc tgtgaagagc ggcgagccgt tgaagataat
       61 cgcaatgcgg ctgccgttcg cttccatttt ggaaatcata tgttgcagga agagcagagc
      121 gccgtcactg gtgcggggcg tgcccacgtg aaaacgtcca tgcgggtcgt tggcttcgtt
      181 cttgatgaag tcttcttcca tcttccaact cacgccaaag ggcgggttgc tcaacatgta
      241 ggtgaattgc tgtcccttga agcggtcatt cttgaaactc gacccctgcc gaatgttctc
      301 ggcattttcg cccatgatga gcacgtccga tttggcgatg gcgtaggtct gctcgttgag
      361 ttcctgcccg tacatcacca cttccacgtc cgcgttgatg tgctcctgaa tgtattcctt
      421 tgccgtgatg agcataccgc ccgtcccaca ggcggggtcg aacaccgagc ggataatccc
      481 tttgcccttc aattcatcct tgtgctcggc aaacatcaaa ctcaccatca agcggatgac
      541 ttcacgcggg gtgtaatgtt cgcccgccgt ctcgttcgac atttccgaaa agcgccgcag
      601 cagttcttca aagatgtatc ccatctcatg gttggtcacg ctggcagggc ttaagtccac
      661 ggcggcgaat ttctccacca gtagatagag cagattgttc ttgattagtt ttgtgatgat
      721 tttttcgagt tggaagttct cgataatctc ccgcacattt ttggaatagc cgtttagata
      781 gttgctgaaa ttgatttcaa tattctttga atcttgcgcg agtcggcgca ggtcataaaa
      841 cgagtgattg tagaaatttg ttccacccgc cgcttgcttc aacacgggga tgattcgctc
      901 ttcgtcgagt gaatccttga actggtcatg cgccttgatg accgcatcct tttgcgcttc
      961 caacacttgg tcaaggcggc gcaagaccac gaagggcaga attacgctgc catactcgta
     1021 agcgtggaag gcgccgcgca gaatgtcgtc cgcgacctgc cagataaagt ttgctttttc
     1081 agagaagttg tttggcattg agtttccttt gcagggtaat tccccgctga atgttctcta
     1141 ggatacagga aaaacggtca aggcgcaagt gtatatacaa acgttagaat accccgtata
     1201 aaccttgtac gcacttaatt aatgtgctat aaagtcgcta tgcgaggcaa tacaggagtt
     1261 ttatttcaag agtgttagac gatttaccaa atctgagtca attgttactc gaacggaagt
     1321 cgagacaaaa tcccacgaag cgggatgatg ttcttgactt ccgttttttt gattcttaat
     1381 cccatgtgaa aaggagacaa tatttgaatg gcaaaaaaga ataaagttgt tatagtgcga
     1441 atgagtgagc aagatgaggc ggaattggaa caactctcca aaaatattaa tcgaagtaaa
     1501 agcggtgcgt tacgttatgc cttgcgagag gtagccagag ccgtgcgaga acaccccgat
     1561 aaaatcaagt tgattcgaac cgtcagagcc agttgatggt aacgaccact ttgtaaactt
     1621 aaacccgccg ttcgagcggc ggg
//
LOCUS       DAOQJE010000866         1639 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_68135_length_1639_cov_5.060606, whole genome shotgun sequence.
ACCESSION   DAOQJE010000866 DAOQJE010000000
VERSION     DAOQJE010000866.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1639)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1639)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1639
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_68135_length_1639_cov_5.060606"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            302..1420
                     /locus_tag="PLT08_17415"
     CDS             302..1420
                     /locus_tag="PLT08_17415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE06309.1"
                     /translation="MDNKTKTALKIGLIGIFPGVSLSIIGPVILGGFAAYSVIKKENI
                     LEKKERIKAGAVAGLVSNLVSGASQIAFSLVLFLISQFLPGEGENRGVAIFISLLYIG
                     VAVIQLFSGLLLGWLGGIYIGYQFDQIGKVSFYKQAILILGTLIVAVNLLFNINQIPT
                     YLENLKTEISAQKGIQHGSLDVNNHSYTPDAKNFSCYFGNMGYGLDQFLYEDDLDLNT
                     ETGYLRVFEEASKQELNITYTPANDTGLYIENFRLELNNWIDEQKTNNPNIIILNGTI
                     ENSITDAVVQSVFLPKETIVAGDNIMPGTSGGYLTGIIFFMKGNYLYTFTLSEYQEIY
                     INPPQKEFGSVKIEQERKANEIISKLLQYKDESCEFLE"
BASE COUNT          535 a          296 c          310 g          498 t
ORIGIN      
        1 gtagacgaac attgggttga agaagtaaaa aagcagttga atagtggtgt ttagacctcc
       61 aaaaacgggc taacaaagcg tgcacccgac gtgtgggatt ctgcggcgtt ttcaagcatt
      121 tttctggctt cgagtttttc ctacatctca gagcgaatcc acacccaccc acacgcgggt
      181 aacgcaaacc gttgggcggc tgtaattaac cctatgctca aaatgatttt taaaacaaaa
      241 attgttcaac tttctattta gttaaaaatc caacatatta cagttataca aatggagaaa
      301 tatggacaac aaaacaaaaa ctgctcttaa gatcgggctt ataggcatat ttcctggcgt
      361 aagtttgtct attattggtc cagtaatttt aggtggattt gctgcttact ctgtaatcaa
      421 gaaagaaaat atcttagaaa aaaaagaacg tattaaagca ggcgctgttg ctggtttagt
      481 ttccaattta gtaagtgggg cttctcaaat tgcattttca ttggtgttat ttttaatttc
      541 tcagttttta cctggagaag gggaaaatcg cggtgttgct atttttattt cccttttata
      601 cattggtgta gcagtaattc agttgttttc aggattatta ttgggatggc taggtgggat
      661 ttatatcggc taccaattcg atcaaatagg gaaagtaagt ttttataaac aagctatatt
      721 aattcttgga acattgatag tcgcagtaaa cctcttattt aatatcaatc aaatacctac
      781 ctacttggaa aatctaaaaa cagaaataag cgcacagaaa ggtattcaac atggaagtct
      841 tgatgttaac aaccattcct ataccccaga tgccaagaac ttctcttgtt attttggaaa
      901 tatgggctat ggcttagatc aatttttata tgaagacgat ctcgacttga atactgaaac
      961 tggctactta cgggttttcg aagaggcgtc taagcaggaa ctaaatatta cttacactcc
     1021 tgcaaatgac acaggacttt atatcgagaa ctttaggtta gagctaaata attggattga
     1081 tgaacaaaag acaaataatc caaacataat tattttgaat gggactattg aaaattcaat
     1141 aaccgatgct gtcgtccaat ccgtcttttt accaaaagaa accattgttg ctggtgacaa
     1201 tattatgcca ggcacaagtg gaggttatct tacaggcata atatttttca tgaaaggcaa
     1261 ttatctttat acattcactc tatctgaata tcaagaaatt tacattaatc ccccacagaa
     1321 agaatttggt tctgtaaaaa ttgaacagga aagaaaagcc aatgaaataa taagtaaact
     1381 ccttcaatac aaggatgaaa gttgtgagtt cttagagtaa agataatgta cgcaatatta
     1441 ttcatcaata aattcctaga tgcacaattt gattgcaata ttaagaaaat ctatcttaaa
     1501 atgccgccca acaaagcgtg cacctgacgc tggggattct gcgcacatcc caagcagttt
     1561 cacacgcctt atcatttttc tggctggacg gcttcgccgt ccctgcccca gcgcaggtaa
     1621 cgcaaaccgt tgggcgttt
//
LOCUS       DAOQJE010000867         1638 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_68221_length_1638_cov_4.200253, whole genome shotgun sequence.
ACCESSION   DAOQJE010000867 DAOQJE010000000
VERSION     DAOQJE010000867.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1638)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1638)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1638
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_68221_length_1638_cov_4.200253"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1638
                     /gene="dnaE"
                     /locus_tag="PLT08_17420"
     CDS             <1..>1638
                     /gene="dnaE"
                     /locus_tag="PLT08_17420"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HNE06310.1"
                     /translation="DGFSNIKKLVKRVKEMNMGSVAITDHGTMFGVIDFYKAAKAEGV
                     KPIIGLEAYMAARGMKDKDSKLDRSSFHLLLLAENDTGYRNLLKIASAAQLDGFYYYP
                     RIDHDFLAAHSEGLICTSGCMSAEIPRALLNENPEEAVRKWNWYYDVFGPDRFFVELQ
                     QHNIPEIIGLNKKLLELGARYSARYVATNDVHYVNQSDAKLQDVLLAIQTNSTLADPD
                     RMRMTDDSYFLRSPEEMSRLFAEVPESLSNTLLIAERCNVDLGFKGYHLPEFPVPEGH
                     TAESYLRSLCEAGAERRYGDDCDSPKVRERLNYELSVIHTMGFDAYFLIVWDLCTFAR
                     DHGIWYNARGSAAGSIVAYTLGITMVDPLEHALIFERFLNPGRISMPDIDLDFRDDRR
                     HEMLEYCARKYGDDKVAQIITFGTMGTKAAIRDVARVMEIPLPEVDRVAKLVPFAAGG
                     TTMAEAKEVKEFKQIYDTDPKMREVIDIAEKMEGTVRNAGTHAAGVVISDKPILEYLP
                     LHRPTSNSEDTPIKSVTQFEMGILDTLGMLKVDFLGLI"
BASE COUNT          385 a          474 c          443 g          336 t
ORIGIN      
        1 gacggcttct cgaacatcaa gaagctggtc aaacgcgtca aagaaatgaa catggggtcg
       61 gtcgccatca ccgaccacgg caccatgttc ggtgtgatcg atttctacaa agccgccaaa
      121 gccgaagggg tcaagcccat tatcgggctc gaagcctata tggcagcacg cggcatgaag
      181 gacaaggact ccaaactaga ccgttcctcc ttccatttat tgctgcttgc ggaaaatgac
      241 accggctacc gcaacctgtt gaagatcgcc tctgccgcgc aactggatgg tttctactac
      301 tacccccgca tcgaccatga ctttttagcg gcacactccg aagggctgat ctgcacctcg
      361 ggctgtatgt ctgccgagat cccgcgcgcg ttattaaatg agaacccgga agaagccgtc
      421 cgcaaatgga attggtatta cgatgtcttc ggacccgacc gattcttcgt cgagttgcag
      481 cagcacaaca tccctgagat cattggcctg aacaagaaac tgcttgagtt gggcgcccgc
      541 tattctgcgc gctatgtcgc caccaatgat gtgcattacg tcaatcaatc ggatgcaaag
      601 ctacaggatg tgctgctggc gatccagacc aactccacac tggcagaccc tgaccgcatg
      661 cgcatgaccg acgactcata cttcctgcgc tcgccggaag agatgagccg actcttcgcc
      721 gaggttccgg aatcattatc caacactttg ctcattgccg aacgctgtaa cgtggacctg
      781 ggattcaaag gttatcacct gcccgagttc cccgtgccgg aaggtcacac tgcagaatct
      841 tatctgcgct cgctgtgcga ggctggagcg gagagacgct atggggacga ttgcgacagc
      901 cccaaagtcc gcgagaggct gaactacgaa ttgagcgtca tccacaccat gggattcgac
      961 gcctacttcc tgatcgtgtg ggacctgtgt acgttcgcac gggaccacgg catttggtac
     1021 aacgcacgcg gttcggcggc gggatccatt gtggcgtaca cgcttggcat cacgatggtc
     1081 gatccgctgg aacatgcgct tatctttgaa cgcttcctca acccggggcg tatatcgatg
     1141 cccgatattg acctggactt ccgcgatgac cgccgacatg agatgctcga atattgcgcg
     1201 cgcaaatacg gcgacgacaa agtggcgcag atcatcacct ttggcacgat gggaacaaag
     1261 gctgccatcc gtgatgtggc gcgtgtgatg gaaattccgc tgcccgaagt ggaccgggtc
     1321 gccaaacttg ttccgttcgc ggcaggcggc actaccatgg cggaggcaaa agaggttaag
     1381 gaattcaaac agatctacga caccgacccg aagatgcgcg aggtgatcga catcgccgaa
     1441 aaaatggaag gtaccgtgcg caatgcaggc acgcacgccg ccggtgtggt gatctcggat
     1501 aagcccatcc ttgaatacct gccgcttcat cgcccgacct cgaactcgga agacaccccc
     1561 atcaaatcgg tgacccagtt cgaaatgggc atcctcgaca cgctgggcat gttgaaagtg
     1621 gacttcctcg gtctcatc
//
LOCUS       DAOQJE010000868         1637 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_68286_length_1637_cov_8.448799, whole genome shotgun sequence.
ACCESSION   DAOQJE010000868 DAOQJE010000000
VERSION     DAOQJE010000868.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1637)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1637)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1637
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_68286_length_1637_cov_8.448799"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1627)
                     /locus_tag="PLT08_17425"
     CDS             complement(<1..1627)
                     /locus_tag="PLT08_17425"
                     /inference="COORDINATES: protein motif:HMM:NF015882.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase"
                     /protein_id="HNE06311.1"
                     /translation="MAKFTREDALEYHRLKGKPGKVAIVPTKPMDTQRDLSLAYSPGV
                     AEPVLEIEKNPADAYEYTSKGNLVAVISNGTAILGLGDRGALASKPVMEGKGVLFKKF
                     ADIDVFDIEVNSHDPDEIIKVVAAIAPTFGGINLEDIKAPECFYIEEELKKMLDIPVF
                     HDDQHGTAIISSAGLANALEIVGKKHEDIRIVISGAGASAISCAELAIRWGVKRENIM
                     LCDSKGVVYKGRKEGMNKYKERFLIETDARTLSDALRGADVFYGLSVANVMTPEMVKS
                     MAKDPIIFAMANPDPEIRPELAKEARKDVIIATGRSDYANQVNNVLGFPFIFRGALDV
                     RAKQINEDMKFAASKALAALAKEDVPDSVIRAYGGEPIKFGREYIIPTPLDPRVLLWE
                     APAVAEMAMKTGMARKTIDIDEYREQLAFRQGKGERIRYFFQNKARSSGGTKRVAFAE
                     GEEQKIIRAAYQIQEEGIATPVLIGRKAIIEKELKALSFDYKPEIVDPDNFGKLEAYA
                     KSFYELRQRKGMTVGDSLKKVRDVNVLGSMMVKM"
BASE COUNT          387 a          476 c          350 g          424 t
ORIGIN      
        1 ccatcttcac catcatcgaa ccaagcacat tcacatcgcg caccttctta agcgagtcac
       61 ctacggtcat ccctttgcgt tggcgcaatt catagaacga tttggcatag gcttcgagtt
      121 tcccaaaatt atccgggtcc acgatctctg gtttgtaatc gaagctgagc gctttgagtt
      181 ctttttcaat aatagccttg cgcccgatca aaacaggtgt ggcaatccct tcttcttgga
      241 tctgatacgc cgcacggatg atcttctgtt cttcgccttc tgcgaacgcc acacgcttcg
      301 ttccaccgga agagcgagcc ttgttctgga agaaataacg aatccgctca cccttgccct
      361 gacggaacgc caactgctcg cgatattcgt caatatcaat ggtcttgcgc gccatgccgg
      421 tcttcattgc catctctgcc acagcgggag cttcccacag cagcacacgc ggatcaagcg
      481 gagttgggat gatgtactca cgcccaaact tgatcggctc gcctccatac gcgcggatga
      541 ctgaatcagg cacatcttct tttgccaacg ctgccagcgc tttcgacgcg gcgaatttca
      601 tatcttcatt gatctgcttg gcacgcacat ccaacgcacc acggaagatg aacgggaagc
      661 ccaacacgtt attgacctga ttggcatagt ctgaacgacc cgtcgcaatg atgacatcct
      721 tgcgggcttc ttttgccagc tctggtcgaa tctctgggtc ggggtttgcc atggcaaaaa
      781 taatcgggtc ctttgccata ctcttcacca tttcgggggt catcacgttc gccacagaca
      841 aaccataaaa aacatcagca ccgcgcaagg cgtcagagag agttcgtgca tcggtttcga
      901 taaggaagcg ttccttatac ttattcatgc cttccttgcg tcccttgtac accacaccct
      961 tgctgtcgca caacatgatg ttctcgcgct tcacccccca acggatcgcc aattctgcac
     1021 atgagatcgc cgaagcgcct gcgccagaaa tcacgatgcg aatatcttca tgctttttac
     1081 caacgatctc caaggcattt gccaaacctg ccgaggaaat aatcgccgtg ccatgttggt
     1141 cgtcatgaaa gacggggata tccaacattt tcttaagttc ttcttcaatg taaaaacatt
     1201 cgggggcttt aatatcttca aggttaatac cgccaaatgt tggggcaatc gccgcaacca
     1261 ctttgatgat ctcatcgggg tcgtgcgagt taacttcaat atcaaaaacg tcaatatctg
     1321 caaacttctt aaagagaacg cctttccctt ccattacggg cttacttgcc aacgctccgc
     1381 ggtcacccag acccagaatc gccgttccat tcgagattac cgccactaaa ttccccttgg
     1441 atgtgtactc atatgcatcc gcaggatttt tctcaatctc tagcaccggc tctgctacac
     1501 cgggagaata cgccaaactt aggtcgcgtt gggtatccat tggcttggtc ggtacaattg
     1561 ccaccttgcc aggcttgcct ttcaaacgat ggtactcaag agcatcttca cgtgtgaatt
     1621 tagccataca gcctcca
//
LOCUS       DAOQJE010000869         1635 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_68456_length_1635_cov_5.298734, whole genome shotgun sequence.
ACCESSION   DAOQJE010000869 DAOQJE010000000
VERSION     DAOQJE010000869.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1635)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1635)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1635
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_68456_length_1635_cov_5.298734"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..403)
                     /locus_tag="PLT08_17430"
     CDS             complement(<1..403)
                     /locus_tag="PLT08_17430"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNE06312.1"
                     /translation="MDNLENIARKITAVLFAQQSLASAGFIAAATLNSIVGKELSHNS
                     SWAGVPTAVYLLAGAFSAFMWGYVYDAIGRRYGLVSGLSTGVVGSSLTFYSIAIHSFP
                     LFLGGMVLMGIANSAVQLGRFAAAEVNKPEHH"
     gene            complement(523..>1635)
                     /locus_tag="PLT08_17435"
     CDS             complement(523..>1635)
                     /locus_tag="PLT08_17435"
                     /inference="COORDINATES: protein motif:HMM:NF007233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase"
                     /protein_id="HNE06313.1"
                     /translation="TPLDPRVLLWEAPAVAEMAMKTGMARKTIDIDEYREQLAFRQGK
                     GERIRYFFQNKARSSGGKKRVAFAEGEEQKIIRAAYQIQEEGIATPVLIGRKDVIENE
                     LKSLSFDYKPEIVDPDNYFKLEAYAKAFYELRQRKGLTVGDALKKVRDVNVLGSMMVK
                     MGDADAFVSGLTYDYPEVIRPALQIHHTAKGVARAAGVYIMIVEDKVFLFTDATVNID
                     PTADDLAEIAILAADYAKKIELDPHVAFLSFSNFGSTPHPLSDKVRKAVNLVKSRRPD
                     LRVDGEMQADTAVVPEIAEERYPFSAVKDANVLVFPSLESANIAYKLLARLGNAKAIG
                     PILLGLGAPVHVLQTGDDVNAIVQIASVAVMDAMGR"
BASE COUNT          437 a          417 c          387 g          394 t
ORIGIN      
        1 tgtgatgctc aggtttgttg acttctgcag cggcaaagcg accaagttgc accgccgagt
       61 ttgcaatgcc catgaggacc attccaccta ggaagagagg aaaagaatgg atggcgatcg
      121 agtaaaaggt caggcttgag ccgacaacac ctgtgcttag cccgctgaca agtccatatc
      181 gtctgccaat agcgtcgtaa acatatcccc acataaaagc agaaaacgcg ccagccaata
      241 aataaacagc tgtgggcaca cctgcccaac tggagttatg acttagttct ttgccaacaa
      301 tggagtttaa ggttgccgct gcgataaacc ccgcagaagc aagggattgt tgagcaaata
      361 atacggctgt gatcttacga gcgatatttt cgagattgtc cacgaaatcg tttcctttgg
      421 gtgaagtcgg tttggattat acacttttga agcggagaga agaaaagacc ccagtaaaga
      481 ttactggggt cttaaacatg atttgactag ccaactatag aactatcttc ccatcgcatc
      541 catcacagct acggaagcga tctgtacaat ggcattaaca tcgtcgccgg tctgaaggac
      601 atgcactgga gcgcctaagc ccaagaggat gggtccgatt gccttggcat tgcccagacg
      661 agccagcaat ttgtatgcga tgtttgctga ttcaagagat gggaaaacca atacattggc
      721 atctttcacc gcactaaacg gatagcgttc ttcagcgatc tcaggcacca cagcggtatc
      781 ggcttgcatt tcaccgtcca cgcgcaagtc aggtcggcga gacttgacga ggttgaccgc
      841 cttgcgaacc ttatctgaaa gcggatgagg cgttgaacca aagtttgaga atgaaagaaa
      901 tgccacatgc gggtcaagct cgatcttctt ggcgtaatct gcggcaagaa tggcgatttc
      961 agccaaatcg tctgcggtcg ggtcaatgtt cacggtggca tcggtgaaga ggaatacttt
     1021 atcttccacg atcatgatgt acacacctgc ggcacgtgcc acaccttttg ctgtatggtg
     1081 aatttgcaat gctgggcgaa tcacctctgg ataatcatag gtcaagccag agacgaaggc
     1141 gtccgcatca cccattttca ccatcattga acccaacaca ttaacatcgc gcaccttctt
     1201 aagcgcgtca ccaactgtca accctttgcg ttggcgcaat tcgtagaacg ccttagcata
     1261 cgcttccagc ttaaagtaat tatcggggtc tacaatttca ggcttgtaat cgaagctaag
     1321 tgacttaagc tcattctcaa tcacatcttt tcgaccgatc aataccgggg tagcaatccc
     1381 ttcttcctga atctgatacg ccgcacggat gatcttctgt tcttcgcctt ctgcaaaggc
     1441 aacacgtttc ttgccgccag aagaacgagc cttattctgg aagaagtaac gaatccgctc
     1501 gcccttgccc tgacggaacg ccaactgctc gcgatattcg tcaatatcga tagttttgcg
     1561 cgccataccg gtcttcattg ccatttcagc tacggcagga gcttcccaca acaacacacg
     1621 cggatctaag ggagt
//
LOCUS       DAOQJE010000870         1623 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_69419_length_1623_cov_3.086097, whole genome shotgun sequence.
ACCESSION   DAOQJE010000870 DAOQJE010000000
VERSION     DAOQJE010000870.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1623)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1623)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1623
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_69419_length_1623_cov_3.086097"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(361..585)
                     /locus_tag="PLT08_17440"
     CDS             complement(361..585)
                     /locus_tag="PLT08_17440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE06314.1"
                     /translation="MKSRVQELAEKINMTKDEFIGEMRKHSCSEPTAIKIWNGAYENF
                     DNFEDNNIQLSNLRKAAFVLRITTGFLIPK"
     gene            844..>1623
                     /locus_tag="PLT08_17445"
     CDS             844..>1623
                     /locus_tag="PLT08_17445"
                     /inference="COORDINATES: protein motif:HMM:NF024873.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease H-like domain-containing protein"
                     /protein_id="HNE06315.1"
                     /translation="MPSLSDKLKSLGGKTGNTLQTPAKPESRSIDSVVAGSFISTPRG
                     EAFVSEQVYDSTYQHGNFPVLSSFPLSLISQWANDPRINQLPITNYAFLDTETSGVSG
                     GTGTYAFLVGVARFMDGKFVIKQFFLRDPAEEPAMLEALIDFLAPCEGLVTFNGKAFD
                     APLLVTRYSLHRIPVPFKNYAHIDLLPLARRLWRDRLPSRALKYIEEHVMGFTRSSDE
                     VPGYEIPWLYFDYLRTGDARPLGGVFYHNAMDVVAMAALLAH"
BASE COUNT          415 a          370 c          320 g          518 t
ORIGIN      
        1 attttagtgc ctagggagcg atacatcgag ttcatttgtt acctccaact gaaatgaatt
       61 aaattgcaac gcgccttttc ggcgcgttaa aatactggtc aagcaatgtc gatctgttat
      121 agagatatcg gtcttttggt aaaaactctg cccatagttt ccccggtaag gctaaggtgg
      181 gtaatatatt tttgctctga aaaagatttc gcggctcaaa ttgccggagc ttaaccacga
      241 ggttatcgtc ttccggaaaa actttttgat aagcaatgaa aactttattg tctgtataca
      301 ttttttatcc cttcaagata aaacctaata tcttttccct ttcagaccgt gacatgctgt
      361 ttattttgga atcaaaaagc ctgtcgtaat tcttaataca aaagccgcct tgcgcaaatt
      421 gcttaattga atgttattgt cctcaaaatt atcgaaattc tcgtatgcac cattccagat
      481 cttgatcgct gtcggttcac tacaactgtg tttgcgcatt tccccgataa actcatcctt
      541 cgtcatattg attttttctg ctaattcttg aactctgctt ttcatatcca ccaatccctt
      601 agagtagcgg ttgaaatatt agaaaaaaac aaaaaacaat agatttttga actttgtttt
      661 taaaacttag taaaaatgca tcttcattac atattcattg caaattgatt gaaagatgct
      721 tacaaaattg caaagtatta aggttattct aatcttattc ctatttaagt caagaacaaa
      781 taatgaggga agtgagagat ttccggtcca aacaccctct caaggcggtt acaatgttat
      841 ttaatgccct cgctttcgga taaactcaaa tcactcggtg ggaagacggg aaatacgctt
      901 cagactcctg ccaagcctga atcccggtct attgattctg tcgtcgccgg gagttttatc
      961 tccacccccc gcggcgaggc gtttgtctct gagcaggtgt acgactctac ctatcaacat
     1021 gggaactttc ccgttctttc ttctttccct ctttctttga tatcacaatg ggcaaacgac
     1081 ccacgcatca atcagttacc aatcaccaac tatgcatttc tagatactga aacttccggc
     1141 gtttctggcg gcacgggcac ttatgctttt cttgtaggag tcgctcgttt tatggatggc
     1201 aagtttgtca tcaagcaatt cttcttgcgt gaccctgccg aagaacctgc catgcttgaa
     1261 gcgctgattg actttcttgc cccatgcgag ggtttggtga ccttcaacgg caaagccttt
     1321 gatgcacctt tgcttgtcac tcgctattcg ctgcaccgca ttcctgtccc attcaagaat
     1381 tatgcccata tagacttact gcccttagcc cgcaggcttt ggcgtgaccg gttgccttct
     1441 cgtgcattga aatatatcga agaacatgta atgggcttca cgcgttcttc tgatgaagtg
     1501 cccgggtatg aaattccgtg gttgtatttt gattatttgc gcacggggga tgcccgtccg
     1561 cttggaggtg tgttctatca caacgccatg gatgtagtgg caatggcagc tttactggca
     1621 cat
//
LOCUS       DAOQJE010000871         1620 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_69656_length_1620_cov_7.346326, whole genome shotgun sequence.
ACCESSION   DAOQJE010000871 DAOQJE010000000
VERSION     DAOQJE010000871.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1620)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1620)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1620
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_69656_length_1620_cov_7.346326"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..582
                     /locus_tag="PLT08_17450"
     CDS             <1..582
                     /locus_tag="PLT08_17450"
                     /inference="COORDINATES: protein motif:HMM:NF013591.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family metallopeptidase"
                     /protein_id="HNE06316.1"
                     /translation="YSNYSLFVAEVASNFNQAMMRGHLLKTIKDKNFLISLIEEAVSG
                     NFFRYFFQMPTLARFELETHQRAERGEPLTADSMQNLMADLFTEGFGPKVKIDRPRVG
                     MVWSTFSHLFADYYVYQYATGISGAHALSARILRGEPNAAEDYLGFLKAGSSKYPLDV
                     LKGAGVDLTTPQAVEETFAVMEGYIDRLEELVG"
     gene            734..>1620
                     /locus_tag="PLT08_17455"
     CDS             734..>1620
                     /locus_tag="PLT08_17455"
                     /inference="COORDINATES: protein motif:HMM:NF025711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CpXC domain-containing protein"
                     /protein_id="HNE06317.1"
                     /translation="MAQTQIACPRCRQLIAANVEQLFDVTADPQAKQRLLSGQVNHAR
                     CPHCGYQGRLATPVVYHDNEKELLLTFFPAELGLPVNEQERMIGPLIKQVTDRLPPEK
                     RKAYLLKPQANLTYESMLETILGKDGITPEMLKEQQDRVQFIEKLMQVTSKDVRTELI
                     KQNVKIIDEQFFALFSRIAQSAMNSGQEQMARVLIDIQTQLLDETEYGRQLKESVGEL
                     EAAQHELQDAGQGLTREKLLDFVLASKSDARIRAYVSLARGGMDYTFFQLLSERIDKS
                     EDDEKTRLEGIREKLLGFVA"
BASE COUNT          423 a          423 c          392 g          382 t
ORIGIN      
        1 tactcaaact attcgctctt tgtcgccgaa gtagcttcca acttcaacca agccatgatg
       61 cgcggacatt tgctcaagac catcaaggat aagaatttct tgatctcact gatcgaagaa
      121 gccgtgagcg ggaacttctt ccgctacttc ttccaaatgc ccaccctcgc ccgcttcgaa
      181 ctggaaacgc atcagcgcgc cgaacgtggc gaacccctca ccgcagactc catgcaaaac
      241 ctaatggcag atttgttcac cgagggcttt ggtcctaaag tgaagattga ccgcccgcgt
      301 gtgggtatgg tttggtctac cttctcgcat ctcttcgcgg actattatgt ctatcagtac
      361 gccacaggca tttctggtgc gcatgcctta tctgccagaa tcttgcgcgg cgaaccgaat
      421 gcagcagaag attacctcgg cttcctcaag gcgggttctt ccaaataccc cttggacgtt
      481 ctcaagggtg ccggtgtaga cttgaccacg ccgcaagccg tggaagagac cttcgccgtg
      541 atggaaggct atatcgaccg cttagaggaa ttagtcgggt agtctgatag cgcattcgtc
      601 tcatcgttaa atcattacgc atcacgggtt acttacatgt aaaacgtggt gcgtaattta
      661 tgtctctcaa gattaattcg tgtaaaatgg gttctgtatt acccaatccc aaatcgtcaa
      721 atcggaaatc gtaatggctc aaacacaaat tgcatgcccc cgttgtagac aattaatcgc
      781 tgccaatgta gagcagttat tcgacgtcac tgctgaccca caagccaaac aacgcttgtt
      841 gagcggacaa gttaaccacg cccgttgccc acattgcggc taccaaggac ggctcgccac
      901 accggtggtg tatcacgaca atgaaaaaga gcttttgctc accttcttcc ccgctgaatt
      961 ggggttgccc gtcaatgaac aggaacggat gatcggtcca ctcatcaagc aggtgactga
     1021 ccgcctccct cctgagaagc gcaaagccta ccttctcaaa cctcaggcaa acctgacgta
     1081 tgagtcgatg ctagaaacca ttctcggcaa agatggcatt acacctgaaa tgttgaagga
     1141 acagcaagac cgcgtccagt tcattgagaa gttgatgcag gtcacatcga aagatgtccg
     1201 cacagagttg atcaagcaga atgtcaagat tattgatgaa caattcttcg ccttgttcag
     1261 ccgcatcgcg caaagcgcta tgaatagcgg acaagaacaa atggcacgtg tgctcattga
     1321 tattcaaact caattgctgg atgaaaccga atatggacgg cagttgaaag aatcggttgg
     1381 agagttggaa gcggctcaac acgagttgca ggacgcgggt caggggttga cgcgcgagaa
     1441 attgctcgac ttcgttctgg catccaagtc tgacgctcgc atccgcgcct atgtctcact
     1501 agcacgtggc ggcatggatt acactttctt ccagcttttg agcgaacgta tcgacaaatc
     1561 ggaagatgac gaaaagaccc gcctcgaagg aatccgtgag aagttgcttg ggttcgtagc
//
LOCUS       DAOQJE010000872         1619 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_69748_length_1619_cov_6.719949, whole genome shotgun sequence.
ACCESSION   DAOQJE010000872 DAOQJE010000000
VERSION     DAOQJE010000872.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1619)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1619)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1619
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_69748_length_1619_cov_6.719949"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(105..1397)
                     /locus_tag="PLT08_17460"
     CDS             complement(105..1397)
                     /locus_tag="PLT08_17460"
                     /inference="COORDINATES: protein motif:HMM:NF013741.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HNE06318.1"
                     /translation="MSISSEIITIIILIVINGVLAMSEAALVASRKARLQQQASEGNK
                     ASALAVKLIEDPNIFLSAVQIGITLIGVLSGAVGGATIAEKLAVFFEKIPYIGPYSES
                     VSLGLVVLVITVLTIWLGELVPKRLGINSPERIAQIVAGPMLFISRVFSPLIKLMSNA
                     TNFVLRIFGVNPSVEPPITEEELQLLIDQGTQAGVFEEAEQDMVEGIFSLGDTRVYSV
                     MTPRTEIVWLDVSDSTEEILEKMSGSPYSRFPVRQDSLETIVGIVKSRDLLVSILSQK
                     QINLKELAKPAHFIPETMLASRALEVLKKNNAEMLLVVDEFGGVQGLLTINDILEEIV
                     GVMEGDEPQATQRQDGSWLLDGMLEVDEFKEIFNLNELPHETEYETLSGFIMTSLGRL
                     PQTADHFEWNNLRFEVVDMDGRRVDKVLVTAKQKTGTD"
BASE COUNT          425 a          380 c          366 g          448 t
ORIGIN      
        1 ggacagacct ttgttttgct gggagatgat tcaggactca tacgggcgaa aattatacca
       61 tcgaacaaat ctacgatttt taggcgattt agctacgaac gggcttagtc tgtgcctgtt
      121 ttttgctttg ccgtgactaa aactttatcg acacggcgcc catccatatc tactacttca
      181 aagcggaggt tgttccactc gaagtggtct gcggtttgtg gcagccgacc gagcgaagtc
      241 ataatgaatc cgctcagggt ttcatactcg gtttcgtgcg gtaattcgtt tagattgaaa
      301 atctctttga attcgtccac ttcaagcatg ccatctaata accacgaacc atcttgacgt
      361 tgtgtggctt gtggttcgtc accttccatc acgccgacaa tttcttcgag gatgtcattg
      421 atcgtcagca aaccttgtac gccgccaaat tcatccacga cgagtaacat ttcagcgtta
      481 ttctttttta gcacctctaa ggcacgtgaa gccaacatgg tttcagggat gaagtgcgca
      541 ggcttggcaa gttctttcag gttgatctgc ttttgagaaa gaatggacac caacaaatct
      601 cgtgatttaa caatgccaac aatcgtctct aatgagtctt ggcgtaccgg aaagcgtgag
      661 tatggactgc cgctcatctt ttcgagaatc tcttccgtgg agtcgcttac atccagccaa
      721 acgatttcag tacgtggagt catcaccgaa tagacgcggg tatcgccaag gctgaaaatg
      781 ccttcgacca tatcttgttc cgcttcttcg aaaacaccgg cttgtgtccc ttggtcaatc
      841 agcaattgaa gttcttcttc tgtaatgggt ggttcgaccg aaggattcac gccaaatatc
      901 cgcaagacaa aattcgtagc gttgctcatt aatttgatca atggtgagaa cacccgcgaa
      961 atgaagagca tcggtccagc cacgatctga gcgatccgtt ctgggctgtt aatgccaagt
     1021 cgcttcggaa cgagttcgcc caaccaaatg gtgagcacgg tgataacaag taccacaagc
     1081 cctaatgaaa cggattcact atagggtccg atatatggga ttttctcaaa gaagacagcc
     1141 aacttctcag caatggtcgc accaccgacc gcaccagaaa gcacgccgat caaggtgatg
     1201 ccaatttgca ctgccgataa aaatatattg gggtcttcta ttaacttaac agccaatgcc
     1261 gaagctttat tgccttcact tgcctgttgt tgcaagcgcg ccttgcgtga ggcaaccaat
     1321 gccgcttcag acattgctaa aacgccattg atgacgatca agattatgat ggtaatgatt
     1381 tcagaactaa tgctcattgc ttcctatggg actctagaat ttcgtttgat actgcttaaa
     1441 aagatacaag ccgcgcataa agaacttcgc ggcgtggaga tttccaccaa gctaattgaa
     1501 acggacaggg ggaactacgg tctggttcta tgggaatgtc ggacatgatg tttcgtgcta
     1561 agtaagtcgc ccttattgta cctgaaagtt acttcttttt tgcgatagta ttttcaata
//
LOCUS       DAOQJE010000873         1616 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_70092_length_1616_cov_2.238309, whole genome shotgun sequence.
ACCESSION   DAOQJE010000873 DAOQJE010000000
VERSION     DAOQJE010000873.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1616)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1616)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1616
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_70092_length_1616_cov_2.238309"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1228)
                     /locus_tag="PLT08_17465"
     CDS             complement(<1..1228)
                     /locus_tag="PLT08_17465"
                     /inference="COORDINATES: protein motif:HMM:NF017539.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NACHT domain-containing protein"
                     /protein_id="HNE06319.1"
                     /translation="MKNKYVTPIVLLLLGIGLFISLYYVSIYIQPILLPSNNYRLVMI
                     GVTFAASFTVLASIAGFLNDALGILDRIYPQANSQENTESLEQPNRRILLNHVENAWI
                     KGVLDASLHGAALLDLGIKQDPKAVTKYPWAIKKESTNELLSEGTSILEIFDSIGMGR
                     SLLILGAPGSGKTTMLLELARGLIARAREDITQPIPMVFNLASWTEKLTLADWLAHEL
                     NNLYSIPLKTATNWVKADRLLLLLDGLDELGKESYAKCVGAINQFRREHGLTSVVVCS
                     RSQVYEGINTKLSLNGAIEVQPLTHMQVEEYIDNFGNQLASLRLALQKDTTLRKIAET
                     PLFLSIMIVAYHGKQVVDISASTNTGSQHKHLFETYIAQMFERNRHTENSLFNTQEVL
                     RWLSWLAHNMAIYNQMP"
     gene            complement(1225..>1616)
                     /locus_tag="PLT08_17470"
     CDS             complement(1225..>1616)
                     /locus_tag="PLT08_17470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE06320.1"
                     /translation="GKETAFIEIKSMPNQGIYQSIKNSLVFGLVFGLLIGIWDWLGFR
                     EPYGVAYALIGTLILGTIQGGYAVINHYSLRLVLSLTNLLPWRLVPFLDHCIDLIFLR
                     RVGSGYIFIHRLLMEHFAEMYTEAKSI"
BASE COUNT          450 a          350 c          361 g          455 t
ORIGIN      
        1 agggcatttg attgtaaata gccatattat gtgctaacca tgaaagccaa cgcaacactt
       61 cttgcgtatt gaataaagag ttttctgtgt ggcgattcct ttcaaacatt tgtgcaatat
      121 atgtctcaaa cagatgttta tgctgactgc ctgtattcgt tgatgccgaa atatcaacaa
      181 cctgttttcc gtgataggcg acaatcatga tacttagaaa cagtggtgtc tctgcaattt
      241 tgcgcagagt agtatccttt tgtaaagcga gtcgtaagga agccaattga tttccaaaat
      301 tatctatata ttcttcaact tgcatgtgtg ttagtggctg aacttcaata gcaccattga
      361 gggataattt tgtgttgatt ccttcataaa cttggctgcg actgcacaca acaacggagg
      421 tcaatccatg ttctcttctg aactggttga tggcgcctac acatttggcg taactttctt
      481 tccctaattc gtccaaacca tccagaagca agagtaatct atcggccttc acccaattgg
      541 tcgcggtctt gagagggata ctgtacaggt tattcaactc atgcgccagc caatcagcaa
      601 gcgtgagttt ctctgtccac gaggcgaggt tgaataccat tgggatgggc tgggtgatat
      661 cttctctggc gcgggcaatc aacccgcggg cgagttctag cagcatggtt gtcttgccag
      721 aaccaggcgc gccgagaatc agtaaggaac gtcccatgcc gatggagtcg aagatttcca
      781 gtatggaagt gccttcagag agcaattcat tcgtggattc ttttttgatc gcccacgggt
      841 atttggtcac cgctttgggg tcttgtttaa tgcccaagtc tagtaaggct gcgccgtgca
      901 gggaggcatc cagcacacct ttaatccatg cgttttcaac atggttaagc aaaatgcgac
      961 ggttgggctg ttcgagggat tctgtattct cttgactgtt ggcttgtgga taaatcctat
     1021 ccaatatacc aagtgcatca ttaagaaacc cagcaatgct ggctaggact gtaaaactag
     1081 cggcaaatgt aacgccgatc atcactaatc ggtaattatt acttggtaat aaaatcggct
     1141 gaatatatat gcttacataa tacaaactta tgaataatcc gatgccaaga agcaaaagaa
     1201 caattggtgt gacatattta tttttcatat tgacttagcc tcggtgtaca tttcagcgaa
     1261 gtgttccatc aacaggcggt ggatgaagat gtatccgcta ccgacgcggc gcaggaagat
     1321 caggtcaatg cagtggtcga ggaaggggac gagtctccac gggaggaggt tagtcagact
     1381 caagacaagg cgcaaactat aatgattgat tactgcatac cccccttgaa tagtacccaa
     1441 aatcaaagta ccaatcaaag catacgcaac accataaggt tctctgaacc ccagccagtc
     1501 ccatattcca atcaataatc caaagactag tccgaaaaca aggctgtttt ttatggattg
     1561 gtaaataccc tgattaggca ttgattttat ctctataaat gctgtctcct ttccaa
//
LOCUS       DAOQJE010000874         1613 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_70293_length_1613_cov_4.930039, whole genome shotgun sequence.
ACCESSION   DAOQJE010000874 DAOQJE010000000
VERSION     DAOQJE010000874.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1613)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1613)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1613
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_70293_length_1613_cov_4.930039"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(93..1331)
                     /locus_tag="PLT08_17475"
     CDS             complement(93..1331)
                     /locus_tag="PLT08_17475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE06321.1"
                     /translation="MKKALITTILFASLLACISTQPSAEDIQNHAATAAWTSVYLTMN
                     AFPTATPPPPTITQTATATIIWPTYSPIPTTPTTATSPYEAEHEAIKKVIYAYFDEIY
                     YMRHTFNVKKFGDVISVAPDGRSFLKTELRKQALDIVWARTNLLRYSSYSYSLDFSEI
                     VVFDAGQKARANLSEGNQVIYEISTLHGINSQSSGIKHIINLRYEQGEWKIIYDVYND
                     NSHRSLYDPTPLPKDVLDGLDKSLIGLGQGKEGPALPKDLTSFIPSDPEQLNRWKDYE
                     TALAEKLMSEYPRDTVFCEWELTEKTLQKINVWASCMTTVTSPDIGNYYFPVVSLPAV
                     INLTPSGEIQSIETPEYGETYLSDIWRLFPNGVWKTAPNVLAMERHLNWRRVHFNEPP
                     LVVLNATAILTPSPTVTPTP"
BASE COUNT          388 a          337 c          346 g          542 t
ORIGIN      
        1 ccgaacattc ttcgcatgtg aattctcctt cacttggtta tttttgtttt gctaaaaata
       61 ttatactgct gaatgaatag gtttttgtga atctatggcg tgggggtgac ggttggtgat
      121 ggcgttagta tggctgttgc attgagcacc accaacggtg gctcattaaa gtgtacccgc
      181 ctccaattta ggtgcctttc catcgctaaa acatttggcg ctgttttcca tacgccattt
      241 gggaataatc tccaaatatc ggagagataa gtttctccat attccggagt ttctatactt
      301 tgtatttctc cgcttggggt aaggttgatt accgctggca gacttacgac cgggaagtag
      361 taattgccaa tatctggtga tgtgacagtc gtcatacagg atgcccaaac atttattttt
      421 tgtaaagttt tctcggtcaa ttcccattca cagaaaacgg tatcccgggg atattcagac
      481 ataagttttt cggccaatgc ggtttcataa tctttccacc tattcaactg ttcaggatca
      541 gatggaataa aagatgttaa atctttaggt aaggcaggtc cttcttttcc ctgccccaaa
      601 cctattaagc ttttgtcaag tccgtctaat acatcttttg ggagcggcgt aggatcgtat
      661 agagagcgat gggaattgtc attataaaca tcatagatga tcttccattc gccctgttca
      721 taacgcagat tgataatatg ctttattcct gatgattgcg agttaattcc atgcaaggtg
      781 gaaatttcgt agataacttg atttccctca gataaatttg cccgtgcctt ttgcccagca
      841 tcgaaaacga cgatttcact aaaatcaagc gagtagctgt aggatgaata cctgagtaaa
      901 tttgtccttg cccatacgat atctaatgcc tgctttctca attctgtctt caaaaacgat
      961 ctgccatcag gagcaactga tatgacatcg ccaaacttct tcacattaaa ggtatgacgc
     1021 atatagtaga tttcgtcaaa gtacgcataa atcacttttt tgattgcttc atgttctgct
     1081 tcataaggcg aggtggcagt tgtgggtgtg gttggtattg gggaatatgt aggccatatg
     1141 attgttgctg ttgccgtttg tgtaattgtt ggcgggggtg gtgtggcagt gggaaatgca
     1201 ttcatggtta agtagaccga tgtccatgcg gctgtggctg cgtgattttg tatatcttct
     1261 gcgctgggct gagttgatat acatgctaac aaactggcaa ataaaattgt cgttataagc
     1321 gcttttttca tgtcatttcc cttcatcttt tgaaattcgt ttgtggggcg tttttgcttg
     1381 tccccgcatg atttggatta tacagagtat ttcgttgcga tgaagtggcg aatgtcataa
     1441 aacaaaaaac ctccgagttc atcaacgact cggaggtttt gactctacct actatttctt
     1501 tcttcttact tctttcttct tcccaccctt cttcacgggc ttcgccgctt tcttcactac
     1561 agcctttttc accttcggct tatccgctgg caactgctcc aactcgctca cca
//
LOCUS       DAOQJE010000875         1613 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_70332_length_1613_cov_2.587933, whole genome shotgun sequence.
ACCESSION   DAOQJE010000875 DAOQJE010000000
VERSION     DAOQJE010000875.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1613)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1613)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1613
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_70332_length_1613_cov_2.587933"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..250
                     /locus_tag="PLT08_17480"
     CDS             <1..250
                     /locus_tag="PLT08_17480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE06322.1"
                     /translation="IKEVAEIDNSCRMAIGFQATGNEPGICDSYKPQVQGESSPSASG
                     CTAKPIQVLACSISADPSYSCSWNSASCSCDCAYVGPK"
     gene            417..809
                     /locus_tag="PLT08_17485"
     CDS             417..809
                     /locus_tag="PLT08_17485"
                     /inference="COORDINATES: protein motif:HMM:NF036989.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE06323.1"
                     /translation="MADSEKITINLGAVDLGKIDLLVEQGHYSNRTDFIRSAIRSQLD
                     KHVLEVQQAVTRYSFGVGAFFHSRADFEKYKSKGDKIKIDIIGIYHIPEDVSVTLALE
                     VVESIKVYGIFQARSEIKDALQKAGKIR"
     gene            806..>1613
                     /locus_tag="PLT08_17490"
     CDS             806..>1613
                     /locus_tag="PLT08_17490"
                     /inference="COORDINATES: protein motif:HMM:NF017760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNE06324.1"
                     /translation="MTSEQQLPENWATRFFTIFAGQAFSLFGSALVQFALIWYLTQKT
                     GSATVLATASLFGMLPQVLLGPIAGTIVDRGNRRLIMILSDSLIAISTLLLAYLFWSG
                     VAEVWHIYVALTIRSAGGAFHYPAMASSTSLMVPEKHLSRVAGANQTLQGLISIVAPP
                     IGALLVAAMSTQNILMIDVITAIMGVTPLLFFTIPQPPRKVEHANAPKPSFLKDLGAG
                     FKYMASWPGLLMLGLLATVLNFLLAPLTALMPLLVTKYFKLGALELGSLDS"
BASE COUNT          444 a          380 c          330 g          459 t
ORIGIN      
        1 tatcaaagaa gtagctgaaa ttgacaattc ctgccgtatg gcaattggtt ttcaagccac
       61 tggaaatgaa ccaggaattt gcgactcgta taagccacaa gtccaaggtg aatcttcacc
      121 atcggcatct ggttgtactg caaaaccaat tcaagtatta gcttgctcta tcagtgctga
      181 cccaagttat tcatgtagtt ggaacagtgc aagttgtagt tgcgattgtg catatgtagg
      241 tcctaaataa tctagtattt tctacaaaaa gaggtcccta agggacccct ttttgtattt
      301 tataccctca aaacaatgat ttaccatcca tatggatggt aaatcattga cataatgtaa
      361 aaaatatgta aaatactcct taataccatc cgtatggatg gtattaagga gtatttatgg
      421 cagattctga gaaaattaca ataaatttag gggctgtaga ccttggaaaa attgacctac
      481 tcgtcgaaca agggcactac tctaaccgca cagacttcat ccgctcggca atccggtcgc
      541 agctagataa gcacgttctt gaagtgcagc aagctgtcac ccgctattcc tttggcgtcg
      601 gtgcattttt tcatagtcga gcagatttcg aaaaatacaa atccaagggc gataagatca
      661 aaatcgatat catcgggata taccatattc cagaagatgt ttccgtaacc cttgctctgg
      721 aagttgtaga gtctataaaa gtgtatggaa tttttcaggc acgtagcgaa ataaaagatg
      781 ctcttcagaa agcagggaaa atccgatgac aagcgaacaa cagcttccag agaactgggc
      841 gacgcgcttc ttcactatct ttgcaggtca ggcattttca ttatttggct ctgcattagt
      901 tcaatttgca ttaatctggt accttaccca aaagaccggc tcggcaacgg tacttgccac
      961 tgcatcttta tttggaatgc tcccacaggt gcttttagga cctatcgcag gaaccatcgt
     1021 ggatcgaggc aaccgtcgct tgattatgat cctatcagac tccctaattg cgatttcaac
     1081 gcttcttctc gcctacttat tctggtctgg cgttgcagaa gtttggcata tctatgtagc
     1141 cttaaccatc cgatctgcgg gcggagcctt ccactacccc gccatggcgt catctacgtc
     1201 tttaatggtt ccagaaaagc atctctcacg tgtagcaggc gccaaccaaa ccctacaagg
     1261 gcttattagt atcgttgctc cgccaatagg cgccttgctt gtagcagcca tgtctacaca
     1321 aaatattttg atgattgatg taattactgc catcatgggc gtcacaccgc tgcttttctt
     1381 caccatccca caaccacctc gcaaagttga gcacgccaat gcaccaaagc ccagctttct
     1441 taaggacttg ggggcagggt tcaaatatat ggcatcttgg cctggtctac tcatgttagg
     1501 tttgttggca actgtattaa acttcctcct agctcccctc acagcgctta tgccattgct
     1561 agttacgaaa tacttcaaat taggtgctct cgaattaggc agtctcgact cat
//
LOCUS       DAOQJE010000876         1608 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_70726_length_1608_cov_3.913715, whole genome shotgun sequence.
ACCESSION   DAOQJE010000876 DAOQJE010000000
VERSION     DAOQJE010000876.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1608)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1608)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1608
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_70726_length_1608_cov_3.913715"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1608
                     /locus_tag="PLT08_17495"
     CDS             <1..>1608
                     /locus_tag="PLT08_17495"
                     /inference="COORDINATES: protein motif:HMM:NF037959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="fused MFS/spermidine synthase"
                     /protein_id="HNE06325.1"
                     /translation="VMLTGGYAYAYWLVKQKKQGWIHLALIGATLALLSALGLVWSSP
                     ITPSADLRPADVSFPIWNILFLLFVSVGLPYFVLASNGTLMQAWFSRAFPEQSYARLY
                     ALSNVGSLFGLLAYPVLVEPSLTLRGQGWAWTGGFLLFALVAGVISYRARTEQGQVVA
                     ATIERPSVALRILWVVLSGTASLFLLSVTNQISQEVAVIPFLWIVPLSVYLLSFILAF
                     SDARWYDRRLYSLLFSLTSIAMLWVLLQAGELNVLIQIAVYTVLLFLACMICHGELYR
                     LRPDAEHLTSFYLMVSVGGAVGGLFVNLVAPFIFTGYWEFYLAWLLTMVLLVVMLLPR
                     FSSRVDLQSRSMSLAFVVGIVLLALGLNPYQNALFAERNFYGVIRVREYDSNALAMIH
                     GATVHGIQILQERDRPTTYYVEESGVGLLLNNHPKRGNMHVGVLGLGIGTVATYGMPG
                     DDYRFYEINPVVVRLAQGEGDYFSFLSDSQAKVTVIEGDARISLENELASGNAQPFDV
                     LVLDTFSSDSIPVHLVTKEAFALYTQY"
BASE COUNT          313 a          348 c          418 g          529 t
ORIGIN      
        1 aagtgatgct cactggcgga tatgcctacg cctattggct ggtgaaacaa aagaaacaag
       61 gatggatcca tcttgcgcta attggagcga cgcttgcgct tctttccgcg ctcgggcttg
      121 tgtggtcgtc cccgatcaca ccctccgccg atcttcgccc cgcagatgtg agcttcccga
      181 tttggaatat tttgttcctg ctctttgtat ctgttgggct cccatacttt gtgctcgcct
      241 cgaacgggac cttgatgcag gcatggttta gtcgcgcttt tcctgaacag tcttacgcac
      301 ggctgtatgc actctcaaat gtgggctctt tgtttggttt attggcatac cccgttttgg
      361 tagagccatc tctcaccctg cgcggacaag gctgggcatg gacgggtggc tttctgttgt
      421 ttgctttggt ggctggggtt attagttatc gcgcccgcac cgaacaaggg caggtcgttg
      481 ccgcaactat agagagacct tctgtagcgt tgcgtatctt gtgggtcgtt ctaagcggta
      541 ccgcttcttt atttttatta tcggtcacca atcaaatttc gcaagaagtg gcggtcattc
      601 cgtttttgtg gatcgtgccg ctttcggtgt atttactttc atttatcttg gcattctctg
      661 atgcgcgttg gtatgacagg cgcttgtatt ctttgctgtt tagccttact agcattgcca
      721 tgttgtgggt cttgttgcag gctggtgagt tgaatgtatt gatacagatc gctgtttata
      781 ctgtgctgct ttttctggca tgtatgatct gccatggcga actctatcgc cttcgcccag
      841 atgctgagca tctcaccagt ttttacctga tggtatcagt aggtggcgcg gtaggcggct
      901 tgtttgtcaa tttagtggcg ccttttatct ttacagggta ttgggaattt tatttagcct
      961 ggttattaac catggttctc ttggtagtga tgctgttgcc gcgtttctca tcccgagtgg
     1021 atttacaatc acgatccatg agtttggcgt ttgtagtggg aattgtgctc ttggcattgg
     1081 ggttgaatcc ttatcaaaac gctttgtttg ccgagcggaa cttttatggt gttatccgtg
     1141 tccgcgaata tgactcgaat gctctggcga tgattcacgg tgccactgtg catggtattc
     1201 aaatcttgca agaacgtgac cgcccaacca catactatgt agaagagagc ggcgttgggc
     1261 tattgctgaa taatcatcct aagcgcggaa atatgcatgt gggtgtatta ggcttgggca
     1321 ttggcactgt agcaacctat ggaatgcccg gcgatgatta tcggttctat gagattaatc
     1381 cagttgtggt ccgtttggca caaggcgaag gagattattt tagcttttta tccgatagtc
     1441 aggcgaaggt gacggttatt gaaggtgatg cgcgtatttc ccttgaaaat gaattagctt
     1501 ctggtaatgc gcaaccattt gacgttttag ttttagatac ctttagcagt gattcgatcc
     1561 ctgtccattt agtgaccaaa gaagcctttg ctttatatac ccaatatc
//
LOCUS       DAOQJE010000877         1607 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_70795_length_1607_cov_6.196521, whole genome shotgun sequence.
ACCESSION   DAOQJE010000877 DAOQJE010000000
VERSION     DAOQJE010000877.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1607)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1607)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1607
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_70795_length_1607_cov_6.196521"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1125)
                     /locus_tag="PLT08_17500"
     CDS             complement(<1..1125)
                     /locus_tag="PLT08_17500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HNE06326.1"
                     /translation="MIIERNDLRNIAIIAHVDHGKTTLVDGLLRQSHTFRENQHVEER
                     VMDSNDLERERGITILAKNTAISLVDPATNQPIKINIVDTPGHADFGGEVERVMNMVD
                     GVLLLVDAAEGPMPQTRFVLKKALEMGHKAIVVINKVDRKDAEPDRVLNETFDLFIEL
                     GASEEQADFPVIYAIGIQQRAGLTPDLGPDLQPLFDTILKHVPAPKVDVDAPLQMLIT
                     TLGYDDYRGVTAVGRVFAGKIKAGQKLARIKLDGTILPETARYLYTFKGLNKVDVEES
                     IAGDIISLAGLEDIAIGETLADPTNPIALPSIKVEEPTVRMTFGVNTSPFSGKEGKWG
                     TSRKLRERLNEELRTNVSLRVADTDSAENFLVSGRGELHLG"
     gene            complement(1248..>1607)
                     /locus_tag="PLT08_17505"
     CDS             complement(1248..>1607)
                     /locus_tag="PLT08_17505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenol monophosphomannose synthase"
                     /protein_id="HNE06327.1"
                     /translation="EKWPVWRKWLSAFGNFYARTILGLPLHDVTTGYRMWRSTTLKQM
                     PFERIQATGYIFLVEMIYLAHCLKFQIGEAPIYFADRRFGKSKMSFKIQMEAAVRIWQ
                     VLWNYRDLRKAGKSARI"
BASE COUNT          422 a          377 c          377 g          431 t
ORIGIN      
        1 acccaagtgc aactcaccgc gtccagagac gaggaagttt tctgctgagt ctgtatctgc
       61 aacgcgcaag gaaacattgg tgcgcaattc ttcattcaag cgttcacgta gtttgcgaga
      121 ggtgccccat ttgccttctt taccagagaa cggtgaagta ttcacaccga aggtcatacg
      181 aactgttggt tcttcaactt tgatggaagg caaagcaata gggttggtgg ggtctgctaa
      241 ggtttcacca atcgcgatat cttccaaacc agcaagggaa ataatatcgc ctgcaatcga
      301 ttcttctaca tcaactttgt tcaagccttt gaatgtgtac aaatagcgag ccgtttcagg
      361 caaaatagta ccatctaatt tgatgcgtgc cagcttctgc ccagccttga tcttgccagc
      421 aaagacacgc ccaaccgccg tcacgccgcg gtaatcatca taaccgaggg tggtaatgag
      481 catctgtaat ggcgcatcca catccacttt cggtgcaggg acatgtttga gaatagtatc
      541 aaacaagggt tggaggtccg gtccaaggtc cggggtaaga cccgcacgtt gttgaatgcc
      601 aatagcgtag ataacaggga agtccgcttg ctcttcggaa gcgccaagtt caatgaacaa
      661 gtcaaaagtt tcattcaaaa cacggtctgg ttcagcatct ttgcgatcga ctttattgat
      721 gaccacaatg gctttgtgac ccatctcaag cgcttttttc aacacaaagc gcgtctgcgg
      781 catgggacct tcggcagcat caacgagcaa aagcacaccg tccaccatgt tcatcacgcg
      841 ttccacttcc ccgccaaaat cggcatgtcc gggagtgtct acgatgttaa tcttgatcgg
      901 ttgattagta gcagggtcta ctaagctaat agcagtgttc ttcgcgagaa tggtgatgcc
      961 gcgttcgcgt tcaaggtcgt tcgaatccat cacacgttct tcgacatgtt gattttcgcg
     1021 gaaggtatga gattgacgta ggagtccatc tacgagagtg gtttttccgt ggtcaacgtg
     1081 cgcaataatg gcgatatttc tgaggtcgtt tcgttcaatg atcattttta ttctttcttc
     1141 aattatatac aggcacaaaa atccctcgac atttcgccga gggggtaatt tttcgttcgg
     1201 cttgcgggca gtattttatc agagagcgtc agtttgagga agggagacta gattcttgcc
     1261 gattttccag ctttgcgcaa atctcggtag ttccaaagaa cctgccagat acgtactgcc
     1321 gcttccatct ggattttgaa agacattttg gattttccaa aacgacggtc cgcaaaataa
     1381 attggggctt caccaatctg aaacttgagg caatgagcta aatagatcat ttcaactaaa
     1441 aaaatgtacc ctgtcgcttg aatgcgttca aaaggcattt gcttgagagt tgtgctacgc
     1501 cacatacgat agcccgtggt tacatcatgt aaggggagtc ctaaaatcgt ccgcgcatag
     1561 aaattaccaa atgcagaaag ccattttcgc caaacgggcc atttctc
//
LOCUS       DAOQJE010000878         1603 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_71157_length_1603_cov_4.011628, whole genome shotgun sequence.
ACCESSION   DAOQJE010000878 DAOQJE010000000
VERSION     DAOQJE010000878.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1603)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1603)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1603
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_71157_length_1603_cov_4.011628"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1241
                     /locus_tag="PLT08_17510"
     CDS             <1..1241
                     /locus_tag="PLT08_17510"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: protein motif:HMM:NF012690.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HNE06328.1"
                     /translation="IGIIHRFMTIAEQTRQIERVKKAESFVVENPLTMSDQATVGEVK
                     RVVEETDTGGILIVDKNEKLVGIITTRDLLFEEDNNKPVTAIMTREVRSAPTNTTLKD
                     AERLLHEHRVEKLPLVDKDGKVTGLVTLKDIMKITKFPKATKDPKGRLAVGAAVGVRD
                     KEMRRVEAALQAGADCIVVDIAHGDSELELDMLKNIRKNFPEAQIIGGNVATADGTKR
                     LIDVGVDAVKVGVGPGSICITRQVAGSGVPQLTAVIECAGAARSSGIPIIADGGIRFP
                     GDVAKAIAAGAQTVMIGSMLAGTDESPGMIMTRRGHRYKASRGMASLAANIERNKREG
                     NDLTREEIEDYVAEGVEAAVPYRGKAREVLTQLVGGLQSGMSYSGAKTIEEFQEKAIF
                     VRMTGAGLRESGPHDVEVLG"
     gene            1316..>1603
                     /locus_tag="PLT08_17515"
     CDS             1316..>1603
                     /locus_tag="PLT08_17515"
                     /inference="COORDINATES: protein motif:HMM:NF012428.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HNE06329.1"
                     /translation="MSDQTQNWIEKDKKQLHPTYHPKTHANPMVIEKGEGVWLHLTDG
                     RKILDGMAGLWNVSAGYGREELAKAAYDQMKDLAFTSNFSGMTNLPSIQLAD"
BASE COUNT          430 a          398 c          437 g          338 t
ORIGIN      
        1 gcattggcat catccatcgc ttcatgacca ttgccgagca aacccgccag atcgagcgcg
       61 tcaaaaaagc cgaatccttc gtcgtggaga atccgcttac gatgagtgac caagccaccg
      121 ttggcgaagt gaagcgcgtt gtcgaagaga ctgataccgg cggcattcta atcgtcgata
      181 aaaatgaaaa gttggtcggc atcatcacca cgcgtgactt gctgtttgaa gaagataata
      241 acaaacccgt cactgcaatt atgacgcgtg aggtccgcag tgcgcccaca aacactacct
      301 tgaaagatgc cgaacgtctt ttgcacgagc atcgcgtaga aaagcttccg ctcgtggata
      361 aagatggcaa agtaaccggc ttggtgaccc tgaaagacat catgaagatc accaaattcc
      421 cgaaagccac caaagacccc aaaggtcgtt tggcagtggg tgccgccgtc ggcgtgcgtg
      481 ataaagaaat gcgtcgtgta gaagctgccc tgcaagctgg tgcagattgc atcgtggtcg
      541 acattgccca cggtgattct gagctggaac tggatatgct caaaaatatc cgcaagaact
      601 tccctgaagc gcagatcatc ggcggaaatg tagcaactgc cgatggaacc aagcgcctca
      661 tcgatgttgg cgtagacgcg gtcaaagtgg gagtgggacc aggttcaatt tgtatcactc
      721 gccaagtggc aggctctggt gtgccgcaat tgacggcggt catcgaatgt gctggcgcgg
      781 ctcgctcaag cggaattccc atcatcgctg acggtggtat tcgtttcccc ggcgatgttg
      841 ccaaagccat cgcggctggc gcacagactg tcatgatcgg ctccatgttg gcaggcaccg
      901 atgaaagccc cggcatgatc atgacccgtc gcggacatcg ttataaggca tcacgtggta
      961 tggcgtcttt ggcggcgaac attgagcgta acaagcgcga aggcaacgac ctgacccgcg
     1021 aagagatcga agattatgtg gcagaaggcg tggaagccgc ggtaccgtat cgcggcaaag
     1081 cacgtgaagt gctcacacag ctcgtcggtg gtttgcaatc gggcatgagc tatagcggcg
     1141 ccaagaccat tgaagagttc caagaaaaag ctatctttgt gcgtatgacc ggcgctggtt
     1201 taagagagtc tggtccgcat gatgtggaag ttttaggtta gaaagttaac aggttagcag
     1261 gttggaacgt tcaaatactc caacttacta tcacctcaac ataattggag aacccatgag
     1321 cgaccaaacc caaaactgga tcgaaaaaga taaaaagcaa ttacacccca catatcatcc
     1381 caagacccat gccaacccaa tggtgatcga aaaaggcgag ggcgtttggt tgcatctcac
     1441 cgatgggcgc aaaattctcg atggcatggc agggctgtgg aatgtcagcg ctggttatgg
     1501 gcgtgaagag cttgccaaag ccgcctacga tcaaatgaaa gatctggcat ttacgtccaa
     1561 tttttctggc atgaccaatt tgccgtccat tcaattggca gat
//
LOCUS       DAOQJE010000879         1601 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_71338_length_1601_cov_2.963131, whole genome shotgun sequence.
ACCESSION   DAOQJE010000879 DAOQJE010000000
VERSION     DAOQJE010000879.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1601)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1601)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1601
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_71338_length_1601_cov_2.963131"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            140..403
                     /locus_tag="PLT08_17520"
     CDS             140..403
                     /locus_tag="PLT08_17520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE06330.1"
                     /translation="MNRHAAMLIADLTAAGAVDPKFIVPAPSHGIYARTGIIKATPET
                     EKAEIAGLPCGVRSETATEARVFVEINGKLTGPYILKPSEVLS"
     gene            400..666
                     /locus_tag="PLT08_17525"
     CDS             400..666
                     /locus_tag="PLT08_17525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE06331.1"
                     /translation="MTETTEAPDIYYGAMEGGHVVSVKSFENIGEQAALILAEKESGL
                     TGWKFRTKGIMNSRGQYVWIMVRSDESADSDSPTWKDMTKGVKP"
     gene            776..1108
                     /locus_tag="PLT08_17530"
     CDS             776..1108
                     /locus_tag="PLT08_17530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE06332.1"
                     /translation="MYKSVTDIKPNYINDIANALRSVAYSVGATMHPEFMRAIKSVAV
                     AYGITENELRNGSNRTGDPNFKEDITAVLKSVRSAAGREIHPEFLRALVAIGLQFGIG
                     SGEIENAA"
     gene            1098..1541
                     /locus_tag="PLT08_17535"
     CDS             1098..1541
                     /locus_tag="PLT08_17535"
                     /inference="COORDINATES: protein motif:HMM:NF020354.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VRR-NUC domain-containing protein"
                     /protein_id="HNE06333.1"
                     /translation="MPRNLPVASESNEQAAFVDTVLQVYRNRADFVRLLFFSTLSGAW
                     IAGSTREAKIRLIAKYKREGWVNGVADILYLQPRGEFAFLAIEMKTAARAKQKNGGAS
                     DDQIEWLKAAKEAGAMTAICHGRDAAWDVFTRYMELPCHEPSLSE"
BASE COUNT          409 a          394 c          441 g          357 t
ORIGIN      
        1 tgcgcgagcg acggatggac gccgagcaac gcggcgacgc cgacgccctt gaagcccttg
       61 cgaatgaata tctcgccatt gacgcgctct cgaattactc cgcgctaatg cgcaaggttg
      121 aagtgttacg aggtgcatta tgaaccgcca cgccgccatg ctaattgcag accttaccgc
      181 cgctggcgcg gttgacccga aatttattgt gccagccccg agtcacggca tttatgcgcg
      241 gacgggcatt atcaaagcca cgccagaaac ggaaaaggca gagatcgccg gattgccgtg
      301 tggcgtgcga agcgaaaccg caaccgaagc ccgcgttttt gtagaaatca acggcaaact
      361 gacgggaccg tatattttga aaccgagcga ggttttatca tgaccgaaac caccgaagcc
      421 cctgacatct actacggcgc aatggaaggc gggcatgttg tgtctgtcaa gtcgtttgaa
      481 aatatcggtg agcaagctgc gctgatactg gcagagaagg agtcaggctt gaccggctgg
      541 aagtttagaa ccaaaggcat tatgaactcg cgcggtcaat acgtgtggat tatggttcgc
      601 tctgacgaaa gcgccgactc tgacagcccg acatggaaag acatgacgaa aggggtcaag
      661 ccgtgaaaga gaaaattcaa attattctga tgatccctat tgcaatcttg cttgctgcgc
      721 tcacctattt agactggtat gtttttggaa atatgccaga aagaaagttt tagtcatgta
      781 caaaagcgta acggacatta agccgaacta tatcaacgac attgcaaacg ccttgcgaag
      841 cgttgcgtac tccgtcggcg cgaccatgca ccctgagttt atgcgggcta tcaaatctgt
      901 tgcggttgcg tatggcatca cggagaacga attgcgcaac ggcagcaacc gcacaggaga
      961 cccaaatttc aaagaggaca tcaccgccgt tttgaagtct gtccgttctg cggctgggcg
     1021 cgagatacac cctgaatttt tgcgggcgct ggttgccatt ggcttgcagt ttgggatcgg
     1081 aagcggagaa attgaaaatg ccgcgtaacc tacctgtcgc ctctgagagc aacgagcaag
     1141 ccgcctttgt ggatacggtc ttgcaggtgt atcgcaaccg tgccgacttt gtacgcttgc
     1201 tgtttttctc gacgctctct ggtgcttgga ttgccggaag cacacgcgag gcgaagatcc
     1261 gcttaattgc aaaatacaag cgcgaaggct gggtaaatgg ggttgcagat attttgtatc
     1321 tgcaacctcg tggtgagttt gctttccttg ccattgaaat gaaaacggcg gcacgtgcca
     1381 aacaaaagaa cggcggcgcg tcggatgatc agattgaatg gctgaaagcg gcgaaagaag
     1441 ccggagccat gaccgcaatt tgtcacggac gggacgcggc ttgggatgtt tttacgcggt
     1501 atatggaact cccttgccat gagccgagtc tgtccgaata aatcatacct accacgaaag
     1561 gtaaaaaata atgagcaatc ccctcccccc ttatctttgg t
//
LOCUS       DAOQJE010000880         1600 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_71397_length_1600_cov_4.582524, whole genome shotgun sequence.
ACCESSION   DAOQJE010000880 DAOQJE010000000
VERSION     DAOQJE010000880.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1600)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1600)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1600
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_71397_length_1600_cov_4.582524"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(141..584)
                     /locus_tag="PLT08_17540"
     CDS             complement(141..584)
                     /locus_tag="PLT08_17540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE06334.1"
                     /translation="MSIEKKKVRYSWHLLLIVAVFLFLYGIAVYDYIMTVSHNQTYFE
                     YLHYNQAVINYFENYPLLPFVFWTTNVFCGMVTLLLMLMKKEIAFYLSLITFVSMFLL
                     DFITFGFLNRWNVIGAKASTIDVLMLVLTLGLCVYNYFLFKKSYK"
     gene            complement(845..1024)
                     /locus_tag="PLT08_17545"
     CDS             complement(845..1024)
                     /locus_tag="PLT08_17545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE06335.1"
                     /translation="MNKNFRLDAWAEYAILSEVDSQFSIEFRRVPIDVEKVKNVWLNS
                     GRPDAERDVSLYYQE"
     gene            complement(1271..>1600)
                     /locus_tag="PLT08_17550"
     CDS             complement(1271..>1600)
                     /locus_tag="PLT08_17550"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HNE06336.1"
                     /translation="GSEVQKRGIMRIAIFSDIHGNCLALETVLADIKKSQIDNLVCLG
                     DAIQGGAQPSETVALLRNLACPTVMGNSDDWLLTGKTTESLLPLHYEIRDWSLSKLSK
                     DDCKFIS"
BASE COUNT          516 a          336 c          292 g          456 t
ORIGIN      
        1 ggggacggcg aagccgtcca gccagaaaaa tgctaaggcg tagaaaactg cttgaaattg
       61 ccgcagaatc cccagcgtca ggtgcacgct ttgttgggcg gcgttttgcc tagaattacc
      121 tgccaattgt ctcacgatat tcatttatat gactttttga aaagaaaata attataaaca
      181 caaagaccta atgtcaatac tagcatgaga acatcaatgg ttgatgcttt tgctccaatc
      241 acattccaac gattgaggaa accaaaagtg atgaaatcca gcaaaaacat tgaaacaaaa
      301 gtaatcaatg acaagtaaaa cgctatttct tttttcatca acattaataa cagtgtaacc
      361 atgccgcaaa aaacattggt agtccaaaac acaaatggaa gtagaggata attttcaaaa
      421 taattgataa cggcttgatt gtaatgaaga tactcaaaat aagtttgatt atgactgacg
      481 gtcataatgt aatcatacac agcaattcca taaagaaata aaaacaccgc aactattaga
      541 agtagatgcc aactataccg aaccttcttt ttttctatgc tcatttcaat caccctaatc
      601 tcattcgtta gtctttatgt ctcctgactg ccgaaccgtt ttttacatcc acaattgaaa
      661 ggaagacaat gttttgcttc actttttagc ggacagccgc ccaacggcat gcgttacctg
      721 cgctggggca gggacggcga agccgtccag ccagaaaaat gctaaggcgt ggaaaactgc
      781 ttgagattgc cgcagaatcc ccagcgtcag gtgcacgctt tgttgggtgc gattttcaaa
      841 acacttactc ttggtaatac aaacttacat ctctttctgc gtctggtctt cctgaattta
      901 gccaaacatt ttttactttt tcaacatcta taggaacacg cctaaattca atactaaatt
      961 gggaatcaac ttcacttaat atagcatatt ctgcccaagc atctaatctg aaatttttat
     1021 tcattcgatt tctgtcatat acacgcccaa cgctacctgg tctgaaataa aaagtatcac
     1081 ctattcggcg caaaagttgc gaatgaacat gtccacctgt gtaaataaaa gactgattat
     1141 cgcctaataa ttcttgaagt tcggtgtccg atgttgtagg taaaatatta tcgtcataag
     1201 attttggcga gccatgaaaa cagaacagac gcttattttt gctgagttca atttcgatgg
     1261 ttggcggaaa ttaagaaata aatttgcaat catctttgga taactttgag agcgaccaat
     1321 cccgtatttc ataatgtaat ggcaaaaggg attcagttgt cttgccagtc aatagccaat
     1381 catcagaatt acccataact gttggacaag ccaaattgcg caaaagggca actgtttcac
     1441 tcggttgtgc tcctccttga atggcgtccc ccaaacaaac aagattatct atctgggact
     1501 ttttgatgtc cgcaagaact gtttctaaag caaggcaatt accatgaatg tcagagaaaa
     1561 tagcaatacg cataatgcct cttttttgaa cttctgaccc
//
LOCUS       DAOQJE010000881         1600 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_71448_length_1600_cov_2.219417, whole genome shotgun sequence.
ACCESSION   DAOQJE010000881 DAOQJE010000000
VERSION     DAOQJE010000881.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1600)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1600)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1600
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_71448_length_1600_cov_2.219417"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            190..633
                     /locus_tag="PLT08_17555"
     CDS             190..633
                     /locus_tag="PLT08_17555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE06337.1"
                     /translation="MRKISKFVLQVTLLGFEMVLLFAFAAIAKPSLDTNIVVRTGLYD
                     SRSGARQHSYNLSWFVDTQQVSLPPASNLTNAAKGLFNSRLDIGGNGYTSYPIAAKGS
                     PLEVTFTVNFKNAVSIPVHFAMPNMEGIVVASKTISSIVGGAGSN"
     gene            766..1041
                     /locus_tag="PLT08_17560"
     CDS             766..1041
                     /locus_tag="PLT08_17560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE06338.1"
                     /translation="MTDQNEVAVYNDCGEKIIVKKISTTKAGNFNLCIHHGDPLLDDE
                     VMFTLSKKDRDTKTVSDKIGAAFHVAGWITSKQERDVIALAILDMTK"
     gene            1158..1448
                     /locus_tag="PLT08_17565"
     CDS             1158..1448
                     /locus_tag="PLT08_17565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE06339.1"
                     /translation="MTKNIVPIQKADSDVVDGVIVSQFPLKVLYGIFRVLDFFLFVVF
                     ITVTGWLLKETAIHPMFGLSVYFVRFLWHGIDTRVRKSLGIETPARNSKKRG"
BASE COUNT          410 a          376 c          363 g          451 t
ORIGIN      
        1 ttgcttaaat atacggtttt acttcgtaca agtcaagttg tacgaagtaa atttacacga
       61 ttttcgaggc tcccccgcaa cctttttttc gattttttat cccttccaac cgaccaaatc
      121 gaactccatt gtgagtctca ggagcctgct cctgtctcta aaatatcatt tcaccttgga
      181 ggtatcgcaa tgcgtaagat ctcgaagttc gttttgcaag tcacgctgct cggatttgaa
      241 atggtgttgc tgttcgcttt tgctgcgatt gcaaagccat cgttggatac gaatattgtt
      301 gttcgtacag gactatatga ctcgcgttct ggcgctcgtc aacattccta caatctgagt
      361 tggtttgtgg acacgcaaca ggtatcactc ccaccagcat cgaacctgac aaacgctgcg
      421 aagggacttt tcaattcgcg actggacatc ggcggtaatg gttacacctc atatcccatc
      481 gcagcaaaag gttctccgct tgaagtgact tttacggtca atttcaagaa cgcagtctct
      541 ataccggttc atttcgcgat gcccaatatg gaaggtattg ttgtggcaag caaaaccatt
      601 tcttcaatcg ttggaggcgc aggatccaac tagcctccaa atgttttacg agagaagcat
      661 acccgcttct ctcgatccct caaagccgaa gctattcgac cttgaaggat cgggagaagt
      721 ttttgattcc tgaccatact acatgatgca aaaggagtaa tgtctatgac agaccaaaat
      781 gaagtcgcag tctacaacga ttgcggcgaa aagattatcg tcaagaaaat aagcaccacc
      841 aaggcgggga acttcaatct ctgcatccat catggcgatc cattgttgga tgatgaagtc
      901 atgttcaccc tctcaaaaaa ggacagagac acgaaaactg tatcggacaa gataggagct
      961 gctttccatg ttgctggttg gattaccagt aaacaggagc gtgatgttat tgccctggca
     1021 attcttgata tgacaaagta ataaaacttc gagagaagcg ggcttgcttc tctcgtccct
     1081 tgagcctcga agacctgatc ttcgggattg aagggttggg agaaataatc ctaccgaaat
     1141 tcttatggag gtatgtaatg acaaagaata ttgttccaat tcaaaaggca gacagtgatg
     1201 ttgtggacgg cgtcattgtg agtcagttcc ccctgaaggt gctgtatggc attttcaggg
     1261 tgctggattt ctttttgttc gttgtcttca tcactgtaac tggctggctg ttgaaggaga
     1321 ctgccatcca cccgatgttc ggtctctctg tctatttcgt gcgtttcctg tggcatggga
     1381 tcgataccag ggttcgcaag agccttggta ttgagacacc agcaaggaat tctaaaaagc
     1441 gcggctaact gcctcgccta cgtcctatcc acccttcgga gaagattacc tatcttctcc
     1501 acccacatat tccaggcgtc cgcctgtttc aaaggacttc cccgattgtg catttaatgc
     1561 ctggtcgggg acggctttta ccggaccact ataaaactat
//
LOCUS       DAOQJE010000882         1599 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_71477_length_1599_cov_7.580959, whole genome shotgun sequence.
ACCESSION   DAOQJE010000882 DAOQJE010000000
VERSION     DAOQJE010000882.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1599)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1599)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1599
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_71477_length_1599_cov_7.580959"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..217)
                     /locus_tag="PLT08_17570"
     CDS             complement(<1..217)
                     /locus_tag="PLT08_17570"
                     /inference="COORDINATES: protein motif:HMM:NF025056.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003953 - NAD+ nucleosidase activity
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="toll/interleukin-1 receptor domain-containing
                     protein"
                     /protein_id="HNE06340.1"
                     /translation="MTGIFISYSRKDSQAAKKLMEGFKSVKLDVWVDWEDIPPAVGWL
                     DQILHGIEQAEVFVFLISPDSIKSEVCK"
     gene            complement(328..>1599)
                     /locus_tag="PLT08_17575"
     CDS             complement(328..>1599)
                     /locus_tag="PLT08_17575"
                     /inference="COORDINATES: protein motif:HMM:NF033679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNRLRE domain-containing protein"
                     /protein_id="HNE06341.1"
                     /translation="SPVVTSITLADPTPNNAELVRFFVNFSEPVNNVDVNDFMMTATG
                     SISNYTILDAYPVGSAYAITVYTGVGDGTLRLDLVDNDSILSATSMPLGGVGIGNGNF
                     SLGNVYVINKNAPFVTSILRADPSPSNAGSVRFSITFSESVSGVDAADFVLSTSGLSE
                     AGVASISGSGTAYMVTVNTGIGSGTLRLDLNDDDSVVDSAGAPVGGIGVGNGNFAAGE
                     TYTIDRNIPLIETVSFSSDGAQDGWVMESKETSNKGGTLNSNAITFRVGDNAQDNQYR
                     SILQFSTGALPDNAIITQMILTMQLETIVGTNPFTTHRNMWVDIRQGAFGSFGPLQIG
                     ALQISDFQAPASLYNAGTIQNNAVGGWYWVNFDSGVFSLVNLKGATQFRLGFQLDDDD
                     DRKDDYLSFFSGNYSVQSARPQLTIKYYIPK"
BASE COUNT          472 a          407 c          332 g          388 t
ORIGIN      
        1 ccttacagac ttctgatttg atcgaatcag gactgatgag aaatacaaaa acttccgcct
       61 gctcaatgcc atgcagaatc tggtccagcc agccaaccgc agggggaata tcctcccagt
      121 ccacccacac atccagtttg acggacttaa agccctccat caactttttg gcagcttgag
      181 agtcttttcg cgagtatgaa ataaaaatac cagtcattta atcaacctta aataagaaaa
      241 aaatctacac ggaattatac cttttacaag gtgggaaaac ctttcagctt tttatgctgg
      301 aaggttatta agaatttctc atcaaaatca cttgggaatg taatacttaa tggtcaactg
      361 tggacgcgcc gattgaaccg agtaattccc gctgaaaaac gagaggtaat catcttttcg
      421 gtcatcgtca tcatccaatt gaaaaccaag gcgaaactga gtagcccctt tcaaattaac
      481 aagtgagaaa acgcccgagt caaaattaac ccaataccac ccccccacgg cattgttctg
      541 aatcgttccg gcattataca aactggcagg cgcttgaaaa tctgaaatct gcaatgcacc
      601 gatctgcaaa gggccaaaac taccaaatgc cccctgacga atatccaccc acatattgcg
      661 atgggtcgta aagggattcg ttccaacaat agtctctagt tgcatcgtca atatcatttg
      721 tgtaatgatg gcattatcag gcaatgcccc tgtggagaac tgtagaatgg agcggtattg
      781 attatcttgt gcattatcac ctacacggaa ggtgatcgca ttagagttca aggttccacc
      841 tttattgctg gtctctttcg actccatcac ccaaccatct tgggctccat ctgaagagaa
      901 agaaacagtt tcaatcaacg ggatattgcg gtcaatcgta taggtctcgc cagcagcgaa
      961 gttcccattc cccactccaa ttcctcccac aggggcgcca gccgaatcaa caacactatc
     1021 atcgtcattc aagtcaagcc ggagggttcc acttccaata cctgtgttga cagtcaccat
     1081 ataggcagtg cctgaaccgc ttatggatgc aacgcccgcc tcactcaaac ccgaagtgga
     1141 cagcacaaaa tcagccgcat ccacaccgct aacggattcg gagaatgtaa tgctgaagcg
     1201 cacacttcca gcgttggaag ggcttgggtc agcacgcagg atggaggtca cgaatggggc
     1261 gtttttgtta atcacataga cattgcccaa tgagaagttc ccgttaccaa tcccaactcc
     1321 acccaaaggc atagacgttg cgctcaagat gctgtcgtta tccacaagat caagacgcag
     1381 ggttccatcc cctacaccgg tatacactgt aatcgcatac gcgcttccga cagggtacgc
     1441 atcaaggatg gtgtaatttg aaatggaacc tgttgcggtc atcataaaat cattgacatc
     1501 tacattgttg accggctctg aaaagttcac aaaaaagcgt acaagctctg cattatttgg
     1561 tgttgggtct gctaaagtaa tggaagtaac caccggcga
//
LOCUS       DAOQJE010000883         1599 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_71481_length_1599_cov_5.402850, whole genome shotgun sequence.
ACCESSION   DAOQJE010000883 DAOQJE010000000
VERSION     DAOQJE010000883.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1599)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1599)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1599
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_71481_length_1599_cov_5.402850"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..940
                     /locus_tag="PLT08_17580"
     CDS             <1..940
                     /locus_tag="PLT08_17580"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNE06342.1"
                     /translation="PEQNPLDYEFLRRLNGSIFLSAIATLVFALLIGILLSRSILRPV
                     RELTKATHHMADGNLGGQVPVRSRDEIGELAQSFNKMSADLARSFNLRKQMTADIAHE
                     LRTPISLILGHAEGVKDGVLKPNAENFEIIREEAERLEHLVSDLRTLSLADAGELSVD
                     FQPVDINNFMSDIHAHYLTLFNQKRITLDLETASATLMANLDPNRFAQVLKNILDNAL
                     RHTPEGGRVELKTQLTGSSIQLSVKDSGDGVHPEEATHLFDRFYRVDESRTRDDGGSG
                     LGLAIAKSIVEMHKGRIWAESEKSKGLKVIIEILKI"
     gene            complement(982..1299)
                     /locus_tag="PLT08_17585"
     CDS             complement(982..1299)
                     /locus_tag="PLT08_17585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE06343.1"
                     /translation="MMNTLFRVLIILVIATIIGGLMYVGVSASNSSTNGFPEFEGGGE
                     RPPFAEGSEFRPDGDNDGERGERSGFGFPGGIIKAVVLMSVAGGIYSGIVWAGKKTKQ
                     TLA"
     gene            complement(1299..>1599)
                     /locus_tag="PLT08_17590"
     CDS             complement(1299..>1599)
                     /locus_tag="PLT08_17590"
                     /inference="COORDINATES: protein motif:HMM:NF025716.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF4405 domain-containing protein"
                     /protein_id="HNE06344.1"
                     /translation="KANANARVNYVLNWLLFIDGILIMLSGIMISEVAIPSLGLALPM
                     NFAWRRLHDMSANLALLIMGLHLAMHWNWIVTTFKRVFLGGFKKKQPVIAAIEEA"
BASE COUNT          441 a          413 c          352 g          393 t
ORIGIN      
        1 accagaacag aatccgcttg attatgaatt tctgcgccgg ttaaacggct ctattttctt
       61 aagcgccatt gctactttgg tctttgcatt attgattggc attctgctgt cgcgcagtat
      121 tttgcgacca gttcgcgagc tgaccaaagc cactcaccac atggcagatg gaaatctagg
      181 cggacaagtt cctgtccgct cgcgagatga gatcggcgaa ttggcgcaat cgttcaataa
      241 gatgagtgca gacttggcgc gctccttcaa cctccgtaaa caaatgaccg ccgatattgc
      301 gcatgaactt cgcacgccca tcagccttat tcttggtcat gctgaaggtg taaaagatgg
      361 tgttctaaaa ccgaatgccg aaaattttga gatcatccgc gaagaagccg aacgcctcga
      421 acatcttgtc agcgatctgc ggactttgtc gctcgccgat gcgggtgaac tttctgtgga
      481 ttttcaacct gtagacatca ataactttat gagcgacatc cacgctcatt acttgactct
      541 attcaatcaa aaacgcatca cactggacct tgaaactgcc tcagcaaccc tgatggcgaa
      601 tctcgacccg aaccgttttg ctcaagtcct aaaaaatatt ctggacaatg cactccgcca
      661 cacccccgaa ggtggacgcg tggaattgaa aactcagctt acaggaagca gcatccagct
      721 ctccgttaaa gatagcggcg atggggttca tcctgaagaa gccactcacc tattcgaccg
      781 tttctaccgc gtagatgaat cccgcacgcg cgacgacggc ggctctggct tgggtctcgc
      841 tattgccaag tctattgtag aaatgcataa ggggcgtatt tgggctgaaa gcgaaaaaag
      901 caaagggttg aaagtaatta ttgaaatcct caagatttaa taaaagagga cgtgctaagc
      961 acgtcctctt ttatattatt tctacgccag cgtttgcttt gttttcttgc ccgcccaaac
     1021 aattcctgaa tagataccgc cagcaacaga cattaatact acagctttga tgatgccacc
     1081 gggaaatcca aagccgctgc gctcaccacg ttctccatca ttgtcgccat ctgggcggaa
     1141 ctcggaacct tcagcgaatg gcgggcgttc acctccgcct tcaaattcag gaaaaccatt
     1201 ggttgatgaa tttgaagcgc tcacacccac gtacattaat ccgccaatga tcgtggcgat
     1261 cactaaaatt attaataccc gaaataatgt attcatcatt aagcttcctc aatggcggca
     1321 ataactggtt gtttcttctt gaaaccgccc aagaagactc gtttgaaggt ggtcacaatc
     1381 caattccaat gcatggcaag gtgcaagccc ataataagca acgcaagatt ggcagacatg
     1441 tcgtgcaatc tgcgccaggc aaagttcata ggcaacgcca gccccaaaga cgggatggca
     1501 acttcagaga tcatgatgcc agacagcatg atgagaatcc catcgatgaa cagcaaccaa
     1561 ttcaacacat agttgacacg tgcattcgca ttcgctttg
//
LOCUS       DAOQJE010000884         1598 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_71558_length_1598_cov_6.652625, whole genome shotgun sequence.
ACCESSION   DAOQJE010000884 DAOQJE010000000
VERSION     DAOQJE010000884.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1598)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1598)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1598
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_71558_length_1598_cov_6.652625"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1295)
                     /locus_tag="PLT08_17595"
     CDS             complement(<1..1295)
                     /locus_tag="PLT08_17595"
                     /inference="COORDINATES: protein motif:HMM:NF028237.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase N-terminal 7TM
                     domain-containing protein"
                     /protein_id="HNE06345.1"
                     /translation="MQPTPLLYPWLLSVTLTFLTGLYAFRYRRHVAAIPFILMCFFAS
                     FWALCYIFELGATSLELKTWGVKLGYIGILGLPLSWTAFALAYSGHTSWITKRNIMLA
                     LIFPIITLIVIFTNQYHHWFFTDFGLQTDSASGLILIYDPLGWWFWLHAVYTYGILIF
                     GSYFLVREYWEKREVYRSQIIINMTAILLPWISNGIVLAGLLPVRIDITSVMFSISIL
                     IFGWGFLRYQLLDILPVAHRAVFESISDSVIVLDPELRIIELNPAAVEMFKLNIGDVL
                     GKSFQSVFRPWVQLNDKALQTHGYHREIILENDGEPVRWLHLYVSALRNGASTTEGHI
                     ITLRDVTSIKENESALAIARDEAMQANSFKSQLLANVSHELRTPLGIILGYTDLIVRR
                     SYGDLTEKQVNILGRIKDSTQYLDGLVSELLDQAQLDSG"
BASE COUNT          477 a          343 c          375 g          403 t
ORIGIN      
        1 tttccgctat cgagttgagc ttggtcgaga agttcagaaa ctagtccatc taaatattgg
       61 gtgctgtctt taatgcgccc cagaatattg acctgttttt cggtcaaatc gccgtaggat
      121 ctacgtacga ttaagtctgt gtacccgagg atgataccga gcggagttcg cagttcgtgg
      181 cttacattgg caagcaattg agatttgaaa ctgtttgcct gcatggcttc gtcacgggca
      241 atggctaatg cagattcgtt ttctttgata ctggtgacat cgcgtaaggt gatgatatgc
      301 ccttcagtag tcgatgcacc attgcgcaaa gcgcttacat aaaggtggag ccaacgtaca
      361 ggctcgccat cgttttcaag gatgatctcg cgatgatacc catgggtttg caaggctttg
      421 tcgtttagct gcacccaggg tctgaagaca gattgaaatg attttccaag cacatcccca
      481 atgtttagtt tgaacatttc cacggcggcg ggattaagct caatgatgcg taattctggg
      541 tcgaggacaa tgaccgagtc ggagatactt tcaaagacgg cgcggtgggc aacaggcaaa
      601 atatccagca gttgatagcg cagaaagccc catccgaaga tcaagatcga gatggagaac
      661 atgaccgagg taatgtcgat gcgaactgga agtagtccag caagcacaat accatttgat
      721 atccacggca gaagaattgc agtcatgttg ataatgatct gagagcgata cacttcgcgt
      781 ttttcccaat attcgcgaac aaggaaataa gagccgaaga tcaaaatacc ataggtataa
      841 acggcatgca accaaaacca ccaacctaaa ggatcataga tcagaattaa tccagaagcg
      901 ctatcggttt gtaacccaaa atctgtgaag aaccagtgat ggtattgatt ggtaaagatc
      961 acaatgagcg tgataatggg aaaaatcaac gccagcatga tgttgcgttt agtgatccaa
     1021 cttgtgtgac cagaatacgc cagtgcaaaa gccgtccatg aaagtggaag accaagaatg
     1081 ccgatatacc ccaatttcac accccatgtt tttaattcca agcttgtagc gcctaattca
     1141 aagatataac aaagcgccca aaatgatgcg aagaagcaca tcaaaatgaa cggaatagca
     1201 gctacatgcc tgcggtaacg aaaagcatat agtcctgtta ggaaagtaag cgttacagac
     1261 agtagccaag gataaagtag aggtgtgggc tgcatttgaa caattataca acttgatacc
     1321 ggggtaaaag aattgcggga acataattag gcgaaagcgt aagaatcacc ctttttgtgc
     1381 atccaagaac taatagccag ctcttctcat ttctttatca cgttttcaag ataaacttgc
     1441 cccaatcttc tttcgctcag aggtctcatg tccacaaccg ttatgcaacc gggcttaccc
     1501 ggtaagtcac gttccaaagt ggtaaccaag cctgccactg atttttatgg gggaattgga
     1561 tgggtcattg ggcttttgct cactcttttc gtttcagc
//
LOCUS       DAOQJE010000885         1597 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_71642_length_1597_cov_7.739948, whole genome shotgun sequence.
ACCESSION   DAOQJE010000885 DAOQJE010000000
VERSION     DAOQJE010000885.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1597)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1597)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1597
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_71642_length_1597_cov_7.739948"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..806)
                     /locus_tag="PLT08_17600"
     CDS             complement(<1..806)
                     /locus_tag="PLT08_17600"
                     /inference="COORDINATES: protein motif:HMM:NF013707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HNE06346.1"
                     /translation="MNKIVNDFSITVGTKNGSGSSTANNTILRSIFKMGIPVSGKNLF
                     PSNIQGLPTWYTIRVNKDGFIARKETSDIVIAMNPDSFAKDLASVTPGGAFFYADDIK
                     QPITRVDIAIYPMPVKTIVKNDPNVPTDFRELVGNMVYVGVLAQVIGMDMEAIRAALT
                     FHFKGKQKPIDMNLNAVKAGAEWAAANLEKKDPFYLEHMNKTEGLIMSDGNTAGAIGS
                     IFGGVQFAGWYPITPASSLAEALNDYLPVLRKREDGKHTYAVVQAEDELAA"
     gene            complement(881..>1597)
                     /locus_tag="PLT08_17605"
     CDS             complement(881..>1597)
                     /locus_tag="PLT08_17605"
                     /inference="COORDINATES: protein motif:HMM:NF019349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FCD domain-containing protein"
                     /protein_id="HNE06347.1"
                     /translation="GFHGRKIRLIGLINFMLRDRISPNISEFLRYLASHPEADDKLPS
                     LNELSRELNISLASLREQLEVARALGFVEVRQKTGTRRLTYSFTPAIRQSLGYALALN
                     DEHFRKFAEMRNHLEAAYWYEAVKQLTDEDKKELNTIITRAWEKLQGTHVQVPHEEHR
                     KLHLTIYKNLDNPFVIGVLEAYWEAYEAVGLNVFTGGYEYLKEVWQYHQRMVESICSG
                     KFEVGYEALVRHTDLLYHRP"
BASE COUNT          442 a          377 c          353 g          425 t
ORIGIN      
        1 gctgcaagtt catcttctgc ctgaacaaca gcataagtgt gcttgccatc ttcgcgttta
       61 cgaagcacag gcaagtaatc atttaaagct tctgccaaag aagaagcagg cgtgatgggg
      121 taccaaccag caaattgaac accaccaaag atagaaccaa tcgctccagc tgtattgcca
      181 tctgacatga tcaagccttc agtcttattc atgtgctcaa gatagaaggg atctttcttt
      241 tcgaggtttg cagcagccca ttcagcacca gctttaactg cattgaggtt catatcaatt
      301 ggcttttgct tgcccttgaa atggaaggtc aacgcagcac ggatggcttc catatccatg
      361 ccgataacct gtgcaagcac gcctacatac accatgttgc caaccaattc acgaaaatcg
      421 gtaggtacat tcgggtcgtt cttgacgatt gtcttaaccg gcattgggta aatggcaatg
      481 tccactcggg tgatgggctg cttgatatcg tctgcgtaga aaaaagcccc acctggcgta
      541 acagaagcga ggtcctttgc gaacgaatcg gggttcatcg caatgacaat gtcactggtt
      601 tccttacggg cgatgaaacc atccttattc acacgaatgg tgtaccacgt aggcagacct
      661 tggatattgg aagggaacag gttcttgcca gagacgggaa tgcccatctt aaagatggac
      721 ctcaaaatgg tgttgttggc agttgaactg ccggaaccgt tcttcgtacc aacggtgatc
      781 gaaaaatcgt tcactatctt attcatggaa ctcctcataa aaattaggtc gatgtacaga
      841 gacggttata tcctcatctg tgcctatcga cgaaaatgac ctagggacga tgatacaaaa
      901 ggtcagtatg acgtacaagc gcctcatacc cgacctcaaa cttgccacta caaattgact
      961 cgaccatacg ctgatggtat tgccacactt ctttcaaata ttcatacccg cccgtgaaca
     1021 catttaaacc cactgcttca taggcttccc aatacgcttc taatacaccg atcacaaaag
     1081 ggttatccaa gtttttgtaa atggtaaggt gtaacttacg gtgttcttca tggggaactt
     1141 gcacatgcgt gccttgaagt ttctcccaag cgcgtgtaat gatggtgttg agttcttttt
     1201 tatcttcatc cgtcaattgc ttaaccgctt cataccaata agcagcttca agatgattac
     1261 gcatttctgc aaatttccga aaatgctcat cattcagtgc caaggcatat cccaaactct
     1321 ggcggattgc tggagtgaag gaataagtca atcgccgcgt tccggtcttt tgacgaactt
     1381 cgacaaagcc aagcgcgcga gcaacttcta gttgttcgcg tagtgatgca aggctaatat
     1441 tcaactctcg gctaagttcg tttaaagagg ggagcttgtc atctgcttca gggtgagacg
     1501 ccagataacg aagaaattcg gaaatgttcg gtgaaatacg gtcacgtagc ataaaattga
     1561 ttaatccgat taatcgtatt ttacgcccgt gaaagcc
//
LOCUS       DAOQJE010000886         1597 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_71654_length_1597_cov_4.300259, whole genome shotgun sequence.
ACCESSION   DAOQJE010000886 DAOQJE010000000
VERSION     DAOQJE010000886.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1597)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1597)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1597
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_71654_length_1597_cov_4.300259"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            147..>1597
                     /locus_tag="PLT08_17610"
     CDS             147..>1597
                     /locus_tag="PLT08_17610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="VCBS repeat-containing protein"
                     /protein_id="HNE06348.1"
                     /translation="MKLSKFLFLFGFALSIISCGSKTANTMLPVVMQATPSAVAQNVI
                     GSNTPTIYPTFTPEAFTSQTSTAVATLDYVKLASRYLTPLPTFTPRTDIPTYAPIWTP
                     ITPKPTPNLSQVDAQKIPSGWEPTPPPIPDVGSLLFSTGEIHNFAADPTYVTSLIKAT
                     TDLMNFTNANQKLFTQYVDTWAPEGRYWFGATWLLEHDFDGDGNPEWLSSVPMYYQPP
                     EPRCCSQLIILFEKDEGIFHPVHFIEKLSTKNATKVLLVDDLDNDGLSEIVVRRVYCG
                     TWCSMDIYVFKWDGEIWTEDSISTVMPDIISFIDYNNDGKTEIALNYFTIYKLDSSYP
                     VREATDIYGWKNGKLVLLEQLLSHNTEPYAIMRDVFSAISSGEFDEALKLAKPAMDKI
                     PQSCKQMETYTAIEVMLIYGVQGKQEAMQSVLAQINTYCNQPDNGFTHAANILWQAYQ
                     QVHDPILACEAMKRFIIEQDPMHDNNPNLVFFDA"
BASE COUNT          483 a          359 c          309 g          446 t
ORIGIN      
        1 agcgtgcacc tgacgctggg gattctgcgg caatctcaag cagtttcaca cgcctaagca
       61 tttttctggt tggacggctt cgccgtcccc gccccagcgc aggtaacgca aaccgttagc
      121 cgctggcatc agtagggtaa tacaaaatga aactatcaaa atttttgttt ttgtttggct
      181 ttgccttgtc aattatttca tgcggttcaa aaaccgcaaa taccatgttg ccagtcgtaa
      241 tgcaagcaac gcctagtgca gtagcacaaa acgtaattgg aagcaacact ccaactattt
      301 accccacttt tacccctgaa gcatttacct cacaaacttc aacggcagtc gccacacttg
      361 attacgttaa attggcttct cgttacctta ctcctcttcc aacatttaca ccaagaactg
      421 atattcccac ttacgcccca atctggacac ctattacacc caagccgaca ccaaatctat
      481 cacaagtgga cgctcaaaaa ataccaagtg gctgggaacc aacgccgccg cctattccag
      541 atgtaggctc actacttttt tcaacaggcg aaattcataa ttttgcggct gacccaactt
      601 atgtgaccag ccttattaaa gccacgacag atttgatgaa tttcaccaac gcaaatcaaa
      661 aactttttac tcaatatgtt gatacgtggg cgccagaagg aaggtattgg tttggggcaa
      721 cttggcttct tgaacatgac tttgatggtg atggaaatcc tgagtggcta tcaagtgtgc
      781 cgatgtatta ccagccccct gagccacgtt gttgttcaca attaattatt ctttttgaaa
      841 aagatgaagg tatatttcat cctgtacact ttattgaaaa actttccact aagaatgcta
      901 caaaagtttt attggtagat gaccttgata atgacggtct ctcagaaatt gtggtaagaa
      961 gggtgtattg tggtacatgg tgcagcatgg acatctacgt tttcaagtgg gatggggaaa
     1021 tctggacaga agattctata agcacagtaa tgcctgatat tatttctttc attgactaca
     1081 ataatgacgg gaaaaccgaa attgctctta attattttac tatctataaa cttgactcgt
     1141 cgtatccagt aagggaagcc acagatattt atggttggaa aaacggaaaa cttgtattgt
     1201 tagagcaact tctttcacat aacacagaac catacgccat tatgcgagat gtgttttctg
     1261 ccatttcaag tggagagttt gacgaagcac tcaagttagc gaagccagca atggataaaa
     1321 tccctcaatc atgtaaacaa atggagactt atactgctat tgaagtaatg cttatttatg
     1381 gcgttcaagg taagcaagaa gccatgcaat ccgtattagc gcaaataaat acatactgca
     1441 accagcctga taacggattt actcatgcag ccaatatttt atggcaagca tatcaacaag
     1501 tccatgaccc aatccttgca tgtgaagcca tgaagcggtt cattattgag caagacccaa
     1561 tgcatgacaa caacccaaat cttgtattct ttgacgc
//
LOCUS       DAOQJE010000887         1594 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_71901_length_1594_cov_6.529565, whole genome shotgun sequence.
ACCESSION   DAOQJE010000887 DAOQJE010000000
VERSION     DAOQJE010000887.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1594)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1594)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1594
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_71901_length_1594_cov_6.529565"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(108..557)
                     /locus_tag="PLT08_17615"
     CDS             complement(108..557)
                     /locus_tag="PLT08_17615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE06349.1"
                     /translation="MIIRPDLGGRIFSGTAGAVLLILACTGLTIPFPGVLFICLGLPL
                     AVFAFGYAFGAAQTQIDEDGVSQRNFFFVPKKFTWNEIVSGKIASETYNSKDSSGWTT
                     RRTRTYALFVNKEGKKIYVNANSSGPEEWWDAMRRISKEKLGERFDG"
     gene            complement(639..1394)
                     /locus_tag="PLT08_17620"
     CDS             complement(639..1394)
                     /locus_tag="PLT08_17620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNE06350.1"
                     /translation="MPVTHITEQFSQPQSSNDVLSVNDLHFSYPDGHVALSGVSLKLY
                     EGDKVALVGPNGAGKSTLMLHLNGILGGKGEVEIAGMRLTRDNLPAIRARVGLVFQNP
                     DDQLFSPTVFEDVAFGPLHMGLPEAEVRARVDTALEAVRMTAYRDRLSHHLSVGEKKR
                     IAIATVLSMNPSLLVLDEPSAGLDPRARRTLIHLLRDLPITMLVSTHDMAMVKELFPR
                     TVVMDEGRVVADGPTSEILENETLLTAHGLEKP"
     gene            complement(1394..>1594)
                     /locus_tag="PLT08_17625"
     CDS             complement(1394..>1594)
                     /locus_tag="PLT08_17625"
                     /inference="COORDINATES: protein motif:HMM:NF014420.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     component T"
                     /protein_id="HNE06351.1"
                     /translation="KIAGNMAGQLFLRSYERSDRIYNAMVARGYVGHLYTLNPHEMKS
                     SDYFSTAAAVAIIFITHILGRF"
BASE COUNT          453 a          400 c          339 g          402 t
ORIGIN      
        1 gggagcgcac tgacaaagct gctgctttga agagtgcggc tggtttgtaa tacaaacttt
       61 ataaaaagag cttctgaaaa ttcagaagct ctttttatat cttcgactta cccatcaaac
      121 cgctccccta acttctcttt ggatatccgc ctcatcgcat cccaccattc ttcgggaccc
      181 gaactattgg cattgacata aatctttttg ccttctttgt ttacaaacaa agcataagtt
      241 cgagtacggc gggtagtcca ccctgagcta tcttttgagt tatatgtttc ggatgcaatc
      301 tttccactta cgatttcatt ccatgtgaat ttcttgggca caaagaaaaa gtttctttgt
      361 gaaacaccat cttcatcgat ctgggtttgt gcagcgccaa atgcatagcc aaaagcgaac
      421 acagccaagg gcaaccccaa acagataaag agcacgccag ggaaaggaat ggttagtcct
      481 gtacaagcca aaataagaag gacggcgcca gcagttccag aaaaaatacg tccgccaagg
      541 tctggtctga taatcatggg atctccttga gattgaaatg cccatattct agtaaaaata
      601 aaaaaagatg gggagtaccc atctttccta ttgattagtt atggcttttc caacccatga
      661 gccgtaagca atgtttcatt ttccagaatt tctgaggttg gtccatcggc aacaacccgc
      721 ccctcgtcca tcactacagt gcgtgggaag agttctttca ccatcgccat atcatgcgta
      781 gacaccagca tagtgatcgg caaatcgcgc aaaaggtgaa tcagcgttct gcgcgcacgg
      841 gggtctaacc cggctgaagg ttcatccaac accagcagac taggattcat ggaaagtact
      901 gttgcaatgg caatgcgctt cttctcgcct acactcaagt gatgagacag acgatctcga
      961 taggctgtca tccgtaccgc ttcaagggca gtgtccactc gtgcgcggac ttccgcttca
     1021 ggcaacccca tatgcaaagg tccaaaggct acatcttcaa atacagtggg cgaaaaaagt
     1081 tggtcatctg ggttctggaa tacgagaccc actctcgcac ggatggcagg caaattgtca
     1141 cgtgtgaggc gcatacccgc aatttccact tcacctttac cacctaagat tccattcaag
     1201 tgcagcatca acgtggactt ccccgcccca ttcgggccca ccaatgcgac cttatctcct
     1261 tcatataact taagggatac gccactcaag gcaacatgcc catcagggta tgaaaaatgt
     1321 aagtcgttta cactcaacac atcgttgcta gactgaggtt gtgaaaattg ttcggtaata
     1381 tgagtaacag gcattagaac cttccaagaa tatgagtgat aaaaataata gctacagcag
     1441 ctgcggtcga aaaataatcg ctggatttca tctcatgcgg attcaacgta tagagatgac
     1501 ccacatatcc ccgagcaacc atggcattgt agatgcggtc actgcgctcg taactacgca
     1561 ggaataattg acctgccata ttcccggcaa tttt
//
LOCUS       DAOQJE010000888         1591 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_72177_length_1591_cov_4.979818, whole genome shotgun sequence.
ACCESSION   DAOQJE010000888 DAOQJE010000000
VERSION     DAOQJE010000888.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1591)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1591)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1591
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_72177_length_1591_cov_4.979818"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..683)
                     /locus_tag="PLT08_17630"
     CDS             complement(<1..683)
                     /locus_tag="PLT08_17630"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HNE06352.1"
                     /translation="MHKHHTFAGLISGLTAASIWGGMYVVSKVVLEVIPPFTLLLIRL
                     IMGAAALGIVIYFRNRKSSTSTPVTKDIFWKSFLVGFVGYGISLGFQFVGTKLSTASN
                     GSLVTSATPAFVLLFAPFLLGEKPTTRRIIALVLSTFGVLAVIDPRTAELSPSLFWGN
                     MSLLAAALTWALYSVLVRKVSQTQDLLTSSTIMLLGGIPSSLAFSLWEINTQGVGEIT
                     LGIIGGLLFL"
     gene            complement(751..1155)
                     /gene="arfB"
                     /locus_tag="PLT08_17635"
     CDS             complement(751..1155)
                     /gene="arfB"
                     /locus_tag="PLT08_17635"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: protein motif:HMM:NF006718.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alternative ribosome rescue aminoacyl-tRNA
                     hydrolase ArfB"
                     /protein_id="HNE06353.1"
                     /translation="MKNLENEISLEYIRASGPGGQNVNKVATAVQLRFDVVNSPSLGS
                     DLKSRLIQLAGKRVNAEGVLILEAKRFRTQEANREDAIAKLYEMIRKASEKPKTRHKT
                     KPTKASKEERMKEKKKRGAVKKLRQTKARFEE"
     gene            complement(1218..>1591)
                     /locus_tag="PLT08_17640"
     CDS             complement(1218..>1591)
                     /locus_tag="PLT08_17640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="2-oxoacid:ferredoxin oxidoreductase subunit
                     beta"
                     /protein_id="HNE06354.1"
                     /translation="YTWGKDHEEPLHEISYIAPRSEIMLEREMNEGEIREVAMHDGST
                     VILKNLEKGYDPTNRMLALQALEEAQQNNWLLTGLIYVNPSKPALTDIYNLVDTPLNR
                     LTETDLRPDRAMIDKINALMF"
BASE COUNT          415 a          365 c          384 g          427 t
ORIGIN      
        1 aggaataaca atccgccaat gatgccaagt gtgatctcgc caacaccttg cgtattgatt
       61 tcccaaagcg agaatgccaa gctggaaggg attccgccaa gcaacatgat cgtgctagag
      121 gtgagaaggt cttgcgtttg cgaaacttta cggaccaaga ccgaatacag tgcccatgtc
      181 aacgccgctg ctaatagact catattgccc cagaaaaggg atggcgaaag ttccgctgtg
      241 cgcgggtcaa tgacggcgag cacaccaaat gtggaaagta caagcgcaat gatgcgtctg
      301 gttgtaggtt tttcaccaag caggaatggg gcaaagagca aaacaaaagc tggcgttgcg
      361 gaggtaacca aagacccgtt cgatgcggtg gaaagcttcg tccctacaaa ttgaaagccc
      421 agcgaaatgc catagcctac aaacccaact agaaagctct tccaaaaaat atcttttgta
      481 accggcgttg aggttgaaga ttttcggttc cgaaaataaa tgacaattcc aagcgctgct
      541 gcccccataa taagacggat aagaagcagg gtgaatgggg gaattacctc caaaaccacc
      601 ttactgacaa cgtacatgcc gccccaaatt gaggcggcag tgagcccaga aatcaaacct
      661 gcgaatgtgt gatgtttatg catagccctc aattatataa tacccacact tgaaaattgc
      721 actttttaga gggcagggca tgtatagaat ctattcttca aaacgtgcct tggtttgacg
      781 taatttcttt accgcgcctc gctttttctt ctctttcatt ctttcctctt ttgaggcttt
      841 ggtgggcttg gtcttgtgac gtgttttggg cttttcgcta gcttttcgaa tcatttcgta
      901 tagctttgcg atagcgtctt cgcggttcgc ttcttgagtg cggaagcgct ttgcttcaag
      961 aatcaaaacg ccctcagcgt tgactcgttt cccagcaagc tgaatcagcc tgctctttaa
     1021 gtctgagccg agtgatgggg aattcaccac atcaaagcgc agttgtaccg ccgtcgcaac
     1081 tttattgacg ttttgtcctc ccggacccga tgcacggata tattccagcg agatttcgtt
     1141 ttctagattt ttcattctgc caaaattata aaataaaaac ccctctcttt tcagagaggg
     1201 gttgaagtgc tagcaaacta gaacatcaaa gcattgatct tgtcgatcat ggcgcggtct
     1261 gggcgcagat cggtttcggt caggcggttg agaggagtat ctacaagatt gtagatatcc
     1321 gtgagggcag gtttggatgg gttgacgtag atcaaaccgg tgagcaacca attattctgc
     1381 tgagcttctt caagagcttg caatgccagc atgcgattgg tggggtcata gcccttttcg
     1441 agattcttga gaataactgt ggaaccatca tgcatggcaa cttcgcggat ttcgccttcg
     1501 ttcatttctc tttcgagcat gatttcacta cgcggcgcga tataagaaat ttcatgcaaa
     1561 ggttcttcgt ggtctttacc ccaagtgtag g
//
LOCUS       DAOQJE010000889         1586 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_72623_length_1586_cov_4.920314, whole genome shotgun sequence.
ACCESSION   DAOQJE010000889 DAOQJE010000000
VERSION     DAOQJE010000889.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1586)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1586)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1586
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_72623_length_1586_cov_4.920314"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            185..1159
                     /gene="lipA"
                     /locus_tag="PLT08_17645"
     CDS             185..1159
                     /gene="lipA"
                     /locus_tag="PLT08_17645"
                     /EC_number="2.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016992 - lipoate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009107 - lipoate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl synthase"
                     /protein_id="HNE06355.1"
                     /translation="MPATPTRDRVSPTTDARPLRRPEWIKVRAPSGENYERVHSLMRS
                     KELHTVCEEAMCPNLGECWGSGTATFLMLGDVCTRTCAFCDIKHGKPALLDWGEAERV
                     AQAVKSMDLKHAVITSVNRDERRDGGAPIFAMVIRRIREVLPGCSIEVLIPDFKGSIE
                     ALKIVMDARPEILNHNVETVPRLFKTIQPQDKYEWAAATLSNAKKLDPDVLTKSGIMV
                     GIGETMDEIKQVMRDQRSWGVDILTIGQYLQPSKKHMPMDRYYSMEEFAELREFGKEI
                     GFKWVESNPLVRSSYHAAEQVRALSVVHRKLYGNGAMSENQLPVVSGK"
     gene            complement(1198..>1586)
                     /locus_tag="PLT08_17650"
     CDS             complement(1198..>1586)
                     /locus_tag="PLT08_17650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE06356.1"
                     /translation="LDELSQADQDYAKQIADLIIAGQIRSLNPANSSVATATKIEALS
                     SIAQALTLSNNKPEGLDREIITFVTFVQARQFPANDCNFGLDGSMLEEYKGGLFNTCD
                     DPSIRVDGIQHWVNGLTLYLEYQAMK"
BASE COUNT          414 a          375 c          417 g          380 t
ORIGIN      
        1 agattcgtaa gggtgataaa atcgaagtcg tcgaagttcg tggattaaga ttgattgtaa
       61 agaaaaagta atatttcgtc attgcgagga gcgtgttttt cgcgacgaag caatctcatg
      121 gctaaactgg agattgcttc gggctaccgc cctcgcaatg acgtaaccgc ctcaaaagga
      181 ccttatgcct gctaccccaa cccgagaccg agtctccccg acaacggatg cgcgtcccct
      241 gcgccgcccc gagtggatca aagtccgcgc gccgtcgggt gaaaattacg aacgtgtaca
      301 ttcattgatg cgttcgaaag aattacatac cgtgtgtgaa gaagccatgt gcccgaacct
      361 cggcgaatgc tggggcagcg gaactgccac cttcttaatg cttggcgatg tgtgtacgcg
      421 tacttgcgcc ttctgtgaca ttaaacacgg caagcccgct ttattagatt ggggcgaagc
      481 cgaacgcgtg gcacaagctg tgaaatccat ggacttgaaa catgccgtca tcacctctgt
      541 gaaccgcgat gaacgccgcg atggtggcgc acccatcttt gccatggtca ttcgccgcat
      601 tcgtgaagta ttgccaggat gttccatcga agtgttgatt cccgatttca aaggcagtat
      661 cgaagccttg aagatcgtga tggatgcccg ccctgaaatt ttgaatcaca atgtggaaac
      721 cgttccgcgt ttgttcaaga ccattcaacc gcaagataaa tatgaatggg ctgccgcaac
      781 tctttccaat gccaagaagc tcgaccccga tgtgttgacg aaatcgggca tcatggtcgg
      841 cattggcgag acgatggatg agatcaagca ggtgatgcgt gaccagcgct cctggggcgt
      901 ggacatcctc accattgggc agtatctgca acccagcaaa aagcatatgc cgatggatcg
      961 ctactacagc atggaagaat ttgctgagtt acgcgagttc ggcaaagaga tcggcttcaa
     1021 gtgggttgag tcgaatccac tggtgcggtc ttcatatcat gccgcagagc aagtgcgagc
     1081 gctgagcgtg gtgcatcgca agttatacgg caatggggcg atgagtgaaa atcaattgcc
     1141 agtggtaagt ggaaaatagt tcataaaaaa gtcctgcgag aaattcgcag gactttttta
     1201 tttcattgct tgatattcca agtataaggt caacccattg acccagtgtt gaattccatc
     1261 cacacgaatg gatgggtcgt cacaagtgtt aaagagacca cctttgtatt cttcaagcat
     1321 agaaccatcc aaaccaaaat tacaatcatt ggcagggaat tggcgggctt gcacaaaggt
     1381 gacaaaggtg atgatctcac ggtccaaccc ttcaggcttg ttattagaca aagtcagcgc
     1441 ctgagcaatg gacgataagg cttcgatctt cgtagcggtt gcaacgctgg aatttgcggg
     1501 gttcaacgag cggatctgcc cggcaatgat taggtctgcg atctgtttgg cataatcttg
     1561 gtctgcctgg ctaagttcat ctaaac
//
LOCUS       DAOQJE010000890         1586 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_72644_length_1586_cov_3.337035, whole genome shotgun sequence.
ACCESSION   DAOQJE010000890 DAOQJE010000000
VERSION     DAOQJE010000890.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1586)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1586)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1586
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_72644_length_1586_cov_3.337035"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            769..1575
                     /locus_tag="PLT08_17655"
     CDS             769..1575
                     /locus_tag="PLT08_17655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE06357.1"
                     /translation="MHLTLGIKQFSTPYHFSGWTDKPNPHHGAKYIRDKSDLFLVSFL
                     AVDIYNKASLHTIFLKKEPRMLEKFISRFAENVFLRVLVVGTTITPIGITTSWLGASY
                     LNSLTGAPFAKTFLGLFIFGLIGAIGSGLIITYAIEHWIIQDKAINSWKWVSIRFVLF
                     LLSSFPVSIGTLISIRIGMQEFPAIVESVYFVEAVTGLFMGCILFTLIEQVIKELKKR
                     ENKLKTEIKELRIEIDQIKRKKQVTEITESDYFQDLQKKVALIKGVKSTA"
BASE COUNT          534 a          279 c          301 g          472 t
ORIGIN      
        1 aattacgcga agagagtaag tctctcgaaa atgaaattta caaattaaaa gcagagtatc
       61 cttctagcaa ggacgccacc taacaaagcg tgcacctgac gctggggatt ctgtggcaat
      121 cccaaacagt ttttcacgcc ttatcatttt tctggctgga cagataagcc atgtccatgc
      181 gctagggcag gtaaagtcaa gccgttaggc gcttatcaca caagcaagaa ttcccacaca
      241 aaacaccaga ggataaaatg actgaagaaa tttatagaaa atgcgttttt agtaaatgga
      301 attgaacagc tttaattaag tgtgattgcg ggaaatcaac ctgcgaacaa gctatatgaa
      361 aaagtaggat ttcaaacttt cgggttacaa cataagtatt tcaaagcagg agaacaatat
      421 atggatcaac tgtttatgca actctttagg gacaattacc aaggcaagaa ataattcatt
      481 actagatgga ttatttcact aatatgtagt tgggcgacct tgctaaataa aatttacaac
      541 aatagtcata aaggattagg tatgaaacca aaaatttcga acgaatggat aaatttttat
      601 atagcaacag gtgttcttct ttcattacca ttaatatgta taatatgtat attcgcaact
      661 tggggtgcat taaatgctct tgtaaatatg tttcagtaaa aattatagtt gatgcagatt
      721 agatgatgag ctcaaaaatc gtgttgctaa aaccgcgcct aacaaagcat gcacttgacg
      781 ctggggatca agcagttttc tacgccttat catttttctg gctggacaga taagccaaat
      841 ccacaccatg gggcaaaata tatcagagat aaaagtgatt tattcctagt ttccttttta
      901 gcagtagaca tctataacaa agcctcacta cacaccatat ttctaaaaaa ggaaccccgt
      961 atgcttgaga aatttatctc ccgttttgca gaaaatgttt ttttgcgtgt cctcgttgta
     1021 ggtacaacta ttaccccaat cggtattaca accagttggt taggcgcttc ctatcttaat
     1081 tcgttaacag gggcaccatt cgcaaaaaca ttcctgggtt tatttatctt cggtttaatt
     1141 ggggcaattg gctcaggtct catcattaca tatgccattg aacattggat catccaagat
     1201 aaagccataa actcatggaa atgggtttct atacgatttg tgctctttct tctttcaagt
     1261 ttccctgtat caattggcac tttaataagt atacggatcg gcatgcaaga atttcctgcc
     1321 attgtagagt ctgtctactt tgtagaggca gtaacaggtt tgtttatggg ctgtatttta
     1381 tttactctca ttgaacaagt tataaaagag ttaaagaaac gtgaaaataa actaaagaca
     1441 gaaattaaag agttacgaat agagatcgac cagattaaga gaaaaaaaca agtaacagag
     1501 attactgagt ctgattactt tcaggattta caaaaaaagg tagctttaat taagggagtt
     1561 aagagcacgg cttaacgatg tttttc
//
LOCUS       DAOQJE010000891         1579 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_73296_length_1579_cov_1.871391, whole genome shotgun sequence.
ACCESSION   DAOQJE010000891 DAOQJE010000000
VERSION     DAOQJE010000891.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1579)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1579)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1579
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_73296_length_1579_cov_1.871391"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            141..1385
                     /locus_tag="PLT08_17660"
     CDS             141..1385
                     /locus_tag="PLT08_17660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE06358.1"
                     /translation="MNIKGQSRKNIIIFLIALILGCTSTPETTLQQNSANTPTPHPTS
                     INTTSVATLRPTLPVQVEDRCLSLEDSLPPDLQLEGVWVRQSGKPYLENLEENLRYRI
                     PLDGGAALHTYNGYWAVSPNGEWLAYLDPILDVSEKITRTKGLTLRVIHSSGYFLSMD
                     YWPIDFQTIQYWVDNENLLLRTDHRNIVLNPFTGKWHEVQKPYWLEEKSDGGSWFNPY
                     QYSPRIDYVIENLEDHSEIKSFVSGESLFGDSAIGFFGTTSWSPDGTMLFLITGEGKN
                     VHILKNDKEILKANLVEIGVLSKSIYDYIDSATWSLDNQRLLLDTDMNSLVLDINENK
                     VYKICFTDKNINKDWIFGNGFFSSKDGKYIIVKRSLVNEKPYVQRIDILIDIENMRAY
                     RLPEIAYKDYIGWLALPETTEK"
     gene            1387..>1579
                     /locus_tag="PLT08_17665"
     CDS             1387..>1579
                     /locus_tag="PLT08_17665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE06359.1"
                     /translation="MNFLKKLFAPREPYKKEYYTFTVKCKRCGETVTGRVDLDNDLSI
                     EYEDGGDVFYARKVLMGENK"
BASE COUNT          513 a          305 c          321 g          440 t
ORIGIN      
        1 ccccaccggc gtggacgaac ccggcacgga cttcggcgca ctgaaggctg gttatgtttc
       61 catcacgccc ctgcaattgg atttgactta catcaaggca atggatgtgt tgaagaagtg
      121 gaatttctaa gggaggcaaa atgaatataa aagggcaatc aagaaaaaat attattattt
      181 tcttgattgc ccttatttta ggttgtacct caacacctga aacaactctg caacaaaata
      241 gtgccaatac tcccactcct catccgacat caataaatac cacgtctgtt gctaccctcc
      301 gacctactct accagttcaa gttgaggata ggtgtttatc tctcgaagac tctttaccgc
      361 cagatttaca acttgagggg gtatgggtga gacaatctgg aaagccttat cttgaaaatc
      421 ttgaagaaaa tttgaggtat agaattcctt tggatggtgg tgcagcatta cacacataca
      481 atggctattg ggctgtttcg ccaaacggag aatggcttgc atacctagat cccatccttg
      541 atgtttctga aaaaataaca agaacaaaag ggctaacatt aagagttatt cactcatcag
      601 gatatttttt gtcaatggat tattggccaa ttgactttca aaccatccag tattgggtag
      661 ataacgaaaa tttactctta agaaccgacc atcgcaacat agtattaaat ccgtttacag
      721 ggaaatggca cgaagtgcag aagccctatt ggttggaaga aaaaagcgat ggagggagtt
      781 ggttcaaccc ctatcaatat agcccacgta tcgactatgt aattgaaaat cttgaagatc
      841 attccgaaat aaaaagtttt gtgtctggtg aaagcttgtt cggggattct gcgattggct
      901 tttttggaac tacctcttgg tcgccagacg ggacaatgct ttttttgata acaggggaag
      961 gcaaaaatgt ccatatatta aaaaatgata aagaaatttt gaaagctaac cttgttgaga
     1021 ttggagtttt gtctaaaagt atctacgatt acatagattc tgccacatgg tcattagaca
     1081 atcaaagact tctccttgac acagatatga attctctggt tttagatatt aatgaaaaca
     1141 aggtttacaa aatatgtttc actgataaaa acatcaataa agattggata tttggcaatg
     1201 gcttcttctc ctcaaaagat gggaaataca tcattgtaaa acgatcgctt gtcaacgaga
     1261 agccttatgt ccaaagaatt gatatactga tagatataga aaacatgcgt gcatacagac
     1321 ttccagaaat tgcttacaaa gattatattg gctggcttgc acttccagaa acaacggaga
     1381 aataaaatga atttcctcaa aaaacttttc gctccccgtg aaccctacaa aaaagaatat
     1441 tacaccttca ccgtcaaatg caagcgctgc ggtgagactg ttactggacg cgtggatctc
     1501 gataacgacc tcagcattga gtacgaagat ggcggcgatg ttttctatgc tcgcaaagta
     1561 ttaatgggcg agaacaaat
//
LOCUS       DAOQJE010000892         1577 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_73410_length_1577_cov_4.234560, whole genome shotgun sequence.
ACCESSION   DAOQJE010000892 DAOQJE010000000
VERSION     DAOQJE010000892.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1577)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1577)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1577
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_73410_length_1577_cov_4.234560"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            98..781
                     /locus_tag="PLT08_17670"
     CDS             98..781
                     /locus_tag="PLT08_17670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE06360.1"
                     /translation="MSENINRQFLTTWSILNFVGLIVGSYFVSLIQSNVGYIGFDFKF
                     SAWKTMLIMLPLGAGIGVFQWFKLRQFGIKLFMWAFATALGFSILVTLYVWVLNFRSH
                     DYREYNIPDWVINTGLAITIPIGGVIIGSLQSIVIRKHISRPCLWIRAYAIGFLLPLI
                     ITPLAYSIKSFILKILYISEFLYFFVDLRWLFFFGFLIIVTVVSISILTSDILLKQSN
                     TNSVTIKAG"
     gene            941..1504
                     /locus_tag="PLT08_17675"
     CDS             941..1504
                     /locus_tag="PLT08_17675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE06361.1"
                     /translation="MNNSFSANDVALLVGIIGLMSGLISAVVTVIVNRLTLQGEIKKL
                     RLTTSNIYAENIQEKRLATYPELYGVLYVFGRTFHDKEIRFDDLVQLHKAIFDWYKVN
                     GILLSPKARDIYYRYYRTIDVLYKKGATSYIQKMSDSNKKHELYKGAVNFNLALQADI
                     GIFDVEFFDRGMRFETYDELNEKTNDD"
BASE COUNT          475 a          306 c          311 g          485 t
ORIGIN      
        1 ctggacggct tcgccgtccc caccccagcg caggtaacgc aaaccgttag cccgctccgc
       61 gcaaaattac tcaaaccatg aataagacag gaatcgcatg tccgaaaata tcaatagaca
      121 atttctgact acatggtcaa ttcttaattt cgttggcttg atagttggaa gttatttcgt
      181 ctccctaatt caatcaaatg taggatatat aggattcgac tttaaatttt ctgcttggaa
      241 aacaatgctt ataatgcttc ctttaggagc aggtattggt gtgtttcaat ggtttaagtt
      301 gagacaattt ggaattaaat tatttatgtg ggcttttgca accgcattgg ggttcagcat
      361 tttagttacg ctatatgttt gggttctaaa tttccgctcg cacgattatc gggaatacaa
      421 tattcctgat tgggtaatta atactggttt ggcgataaca ataccaattg gcggagtaat
      481 tattggcagt cttcaatcaa tcgtcatcag aaaacacatt tcaagaccat gtttatggat
      541 tagagcatat gccatcggat ttcttttacc actaataatt actcctctcg cctattcgat
      601 aaagtcattt attctcaaaa ttctttatat ttctgaattt ttgtattttt ttgtggactt
      661 acgctggcta tttttttttg gatttctgat tattgtcact gtcgtaagca tatcaatatt
      721 aacaagcgat atcctgttaa aacaatcaaa cacaaattcc gttacaataa aagcgggcta
      781 acaaagcgtg cacctgacgc tggggattct gcggcaatct caagcagttt tcacgcctta
      841 gcctttttct ggctggacgg cttcgccgtc cccgccccag cgcaggtaac gcaaaccgtt
      901 ggctggctgg cgcaggaaaa acaatcaaaa cgagaagatt atgaataatt cattttctgc
      961 aaacgacgta gcattgttag tagggataat tggtttaatg tctggattaa tcagtgccgt
     1021 tgtaacagtt atcgttaatc gtcttaccct tcaaggtgaa attaaaaaac ttagattgac
     1081 aaccagcaat atttacgctg aaaatattca agaaaaaaga ctcgcaactt acccagaact
     1141 atacggcgtt ttatatgtgt ttggcaggac atttcacgat aaagaaatta ggtttgacga
     1201 cttggtgcaa cttcataagg caatctttga ctggtataaa gtcaacggca ttttattgag
     1261 tccaaaagca agggatattt attacaggta ttatcgaacg atagatgtcc tttacaaaaa
     1321 aggcgcaaca tcctacattc aaaaaatgag cgacagcaat aagaaacatg agttatacaa
     1381 aggtgccgtt aatttcaatc tcgctcttca agcagatata ggaatttttg atgtagagtt
     1441 ttttgacagg ggaatgcgtt ttgagacgta cgatgaactt aatgaaaaaa ctaatgatga
     1501 ttaatggttt ctcgttccca atacgccagc caacaaagcg tgcacctgac gctggggatt
     1561 ctgcgcacat cccaggc
//
LOCUS       DAOQJE010000893         1574 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_73717_length_1574_cov_6.832785, whole genome shotgun sequence.
ACCESSION   DAOQJE010000893 DAOQJE010000000
VERSION     DAOQJE010000893.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1574)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1574)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1574
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_73717_length_1574_cov_6.832785"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            125..649
                     /locus_tag="PLT08_17680"
     CDS             125..649
                     /locus_tag="PLT08_17680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE06362.1"
                     /translation="MLRNFFYSKNSATKWLKKVDPNEELVFSSVASILETKESAPVGQ
                     SFKRFFQHSGVVLITQNQIAVKNSIFFLPTVFFGFLVFVSLLTILSKDENNLFGGILG
                     LVVFSTPILRQCVPYKKQIPLQDIIKVKLSSVSGFTGKHQLFTITLKDKTLNIVPTQT
                     MPGEVIELLSSPKK"
     gene            861..1511
                     /locus_tag="PLT08_17685"
     CDS             861..1511
                     /locus_tag="PLT08_17685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE06363.1"
                     /translation="MSTFLLILKILGILLSSASAIGSERLKKIEFRLRIFLSRENLDI
                     FFTSKIIVTVLSFIKQREDVQKNFLSESHGSLSISLRKRYLEWGRRILKKDIPRPKNQ
                     VASTIISIFLTLARRTFFITFIIIFIVLFLITMVGNFIEFLISLLYFLTTLLVSLAAM
                     LIIVVLLEILKMSIWVVSRPYTWFDLEMRKREIESTLVIIGAVVAIAAELLGNYYH"
BASE COUNT          476 a          305 c          292 g          501 t
ORIGIN      
        1 tatcattttt ctggttggac ggcttcgccg tccctgcccc agcgcaggta acgcatgccg
       61 ttaggcgctt ggtcagtagg attgcctcac tgaataaaca aaaaacaaag gtggaattat
      121 ccaaatgctg cgaaactttt tttatagtaa aaactctgca acaaaatggc taaaaaaagt
      181 cgatcctaat gaggagcttg ttttttcttc tgtcgcatct atattagaaa ctaaagagtc
      241 tgctcctgtt ggtcagtcct ttaaacgttt tttccaacat tctggggttg tattgattac
      301 tcagaaccaa atcgctgtaa aaaacagcat cttctttctc cctactgttt tttttggttt
      361 cttagttttc gtttcgctac ttacgatttt aagtaaagat gagaataatc ttttcggtgg
      421 catattgggt ttagtagttt ttagtacccc aattttgaga caatgtgttc catataaaaa
      481 acaaatccct ttacaggaca tcattaaagt gaaactcagt agtgttagtg gtttcacagg
      541 taaacatcaa ctatttacta ttacattgaa agataaaacg cttaacattg tccctactca
      601 aaccatgccc ggtgaagtca tagaattgct ttcatcacct aaaaaatgag tttatactat
      661 tgtcataccg ataagagtct tgcgttcatt cagcgcctaa caaagcgtgc acctgacgct
      721 ggggattctg cgcacatccc aaacattttt cacgccttag cctttttctg gttggacggc
      781 ttcgccgtcc aagccccagc gcaggtaacg caatccgttg ggcagttcct tgtaaaaaaa
      841 cggagaaccg caaataaaga atgagcacat tcctattgat attgaaaata ctgggaattt
      901 tattatctag cgcctcagca attgggtcag agcgccttaa aaaaatcgaa tttagattgc
      961 gtatatttct gagcagagaa aacttagaca tattttttac atctaagata atcgttacag
     1021 tgctttcatt cataaaacaa agagaagatg tacaaaagaa ttttttgtct gagtcgcatg
     1081 gttctctctc aatttctttg cgaaaaagat atcttgaatg ggggagacga attcttaaga
     1141 aagatattcc aagaccaaaa aatcaagtag cgagtaccat catttcgatt tttctcacat
     1201 tagcacgaag aacttttttt ataaccttca tcataatttt catcgtattg ttcctcataa
     1261 caatggttgg aaattttata gagtttctta tttcattact gtactttctt accaccttat
     1321 tagtatcctt agcggcaatg ttgattattg tggtgcttct cgaaattctg aaaatgagta
     1381 tctgggtagt atcaagacca tacacttggt ttgatttgga aatgagaaaa agagaaatag
     1441 aaagcaccct tgttataatt ggagcagttg ttgcaattgc cgcagaattg cttggtaatt
     1501 attaccatta aaaatgaaca ctgcccaaca aagcgtgcac ctgacgctgg ggattctgcg
     1561 gcaatctcag gcag
//
LOCUS       DAOQJE010000894         1573 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_73822_length_1573_cov_7.110672, whole genome shotgun sequence.
ACCESSION   DAOQJE010000894 DAOQJE010000000
VERSION     DAOQJE010000894.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1573)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1573)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1573
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_73822_length_1573_cov_7.110672"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..613
                     /locus_tag="PLT08_17690"
     CDS             <1..613
                     /locus_tag="PLT08_17690"
                     /inference="COORDINATES: protein motif:HMM:NF041359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="GntG family PLP-dependent aldolase"
                     /protein_id="HNE06364.1"
                     /translation="NMCGGVVLSVEYTKQVGQLAKEYGLKFHIDGARIFNAAAALNVD
                     IKELVAPADSVMFCLSKGLVAPIGSMLVGSKEFIAKAHRLRKMLGGGMRQVGVMAAAG
                     LVSLDKMTGRLKQDHARAKNLYEGLKQVKGLRLEAEPASNMVYFSLADHITLTENQIC
                     AEMKKHGVMVDWSAPRQFRLVTHYWVDDAGVEKTVKALGEVLK"
     gene            complement(614..>1573)
                     /locus_tag="PLT08_17695"
     CDS             complement(614..>1573)
                     /locus_tag="PLT08_17695"
                     /inference="COORDINATES: protein motif:HMM:NF014069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HNE06365.1"
                     /translation="KNLVTGIVLFNTTGEVIAEVLVNDDGKTANLEHPMSLIQDAMKS
                     GQMIYVSPDESTIRTCLPIQSPIRGYGAIWMDTVDDPSKPSTNPNDLQALVNQSAIAL
                     ERSLLLVESRRQAKEIKEAYDILEVTYDQTLASLTAALDARDRETEGHSLRVTKLAVR
                     LGETLGYKAKQLKILERGSLLHDIGKIGISDTILHKPGPLTEAEWEIMRLHPDIGAKI
                     VEGIPFLEETIPLIRHHQERWDGSGYPAKLKGTDIPELARLFSVIDAFDALTSNRPYR
                     QKISIKEALEYLKEMSGIHFDPDMVINFEALINQNPEIVQSGD"
BASE COUNT          369 a          313 c          450 g          441 t
ORIGIN      
        1 gaacatgtgc gggggtgttg tcctttctgt ggagtataca aagcaggttg gtcaacttgc
       61 aaaagagtat ggactgaaat tccatattga tggtgcgcgt attttcaacg cggcggctgc
      121 tttgaatgtg gatatcaaag aacttgttgc cccagccgat tcggtgatgt tctgtttgag
      181 caagggcttg gtcgcgccga ttgggtcgat gttagttggc tcaaaggaat ttattgcaaa
      241 ggcgcaccgt ctgcgtaaga tgttgggtgg ggggatgcgg caggttgggg tgatggcggc
      301 ggcaggattg gtgtcgctgg ataagatgac ggggcggctt aagcaggatc atgcccgggc
      361 gaagaatctg tatgaaggct tgaagcaggt gaagggatta agactcgagg cggagcctgc
      421 atccaatatg gtgtatttca gtttggcaga tcatattacg ctgaccgaaa atcaaatctg
      481 cgctgaaatg aaaaaacacg gcgtgatggt ggattggtct gcgccacgac aatttcgctt
      541 ggtgacgcat tattgggtgg atgatgcggg tgtggaaaaa accgtgaaag cccttggcga
      601 agtgctgaaa taatcaatca cctgattgaa caatttcagg gttttgattg atgagcgctt
      661 cgaaatttat gaccatgtct gggtcaaaat gaatgccaga catttctttc aaatattcaa
      721 gtgcttcttt gattgatatt ttctgacgat aggggcgatt gcttgtcagt gcatcaaagg
      781 catcgatgac agagaaaagg cgtgctaact ctggaatatc ggtgcctttt agtttggcag
      841 ggtagccgct accgtcccaa cgttcttggt gatggcggat aagtggaatg gtctcttcaa
      901 ggaagggaat gccttcgacg atctttgcac ctatatctgg gtgcagacgc ataatttccc
      961 attctgcttc tgtcagcgga cctggtttat gcaagatggt atcgctgatg ccgatttttc
     1021 caatatcatg taaaagagaa ccgcgctcga gaattttgag ttgcttggct ttatacccta
     1081 aggtttctcc caaccgtacg gcaagcttgg tcacacgcaa actgtgacct tctgtctcgc
     1141 ggtctcgggc atctaatgca gcagtaagag aagcgagcgt ttggtcgtag gtaacttcga
     1201 ggatgtcgta cgcttctttt atttcttttg cttgccggcg cgactcgacc agaaggagtg
     1261 agcgttccaa ggctatggca gattgattaa caagggcttg caggtcattt gggttggtcg
     1321 atggtttgga tgggtcgtcc actgtatcca tccaaatggc gccgtatccg cgaatgggag
     1381 attgaatggg caagcaagtc cggatcgtcg attcatctgg tgagacatag atcatctgac
     1441 ctgatttcat tgcatcttga attaggctca tggggtgttc taggttggca gtcttgccat
     1501 cgtcattgac caacacctct gcaatgacct ctcctgttgt attgaagagc acgatacccg
     1561 tcaccaagtt ttt
//
LOCUS       DAOQJE010000895         1572 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_73944_length_1572_cov_3.318392, whole genome shotgun sequence.
ACCESSION   DAOQJE010000895 DAOQJE010000000
VERSION     DAOQJE010000895.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1572)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1572)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1572
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_73944_length_1572_cov_3.318392"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            118..768
                     /locus_tag="PLT08_17700"
     CDS             118..768
                     /locus_tag="PLT08_17700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE06366.1"
                     /translation="MRPFLQKYSIAIFILVALIITVGMVVWGNNPNSPAIIRDLGLNL
                     VSEVFGILLTVLIIDVMIKRNEDSRWLPTKHITYAKITEIALEIQYYLNTEDERKKQP
                     IVVFFGVTDARPDYHQSVSIEKFEEIVNQYHKRVREIIDLTGMLLEPEIQTHLNALER
                     NLEKTAGLFVKYKDLDKLKNPLLPIINFVEVKESVDKIVSLMLEKSTGKIPFHERT"
     gene            1005..1493
                     /locus_tag="PLT08_17705"
     CDS             1005..1493
                     /locus_tag="PLT08_17705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE06367.1"
                     /translation="MEKASIIFSVLIHSDEFMYLFWGALGVINMSLGTYLLKSKGLGE
                     KELAEISPKFKNINSDYMVFSLFAIMLIIAPVLLLMSGADKWAMGNYGRRFYPALVFF
                     CSGYGIYQALFAIFKDVYPMGKISYFIYDDKKVIRRAAKIHIISSVVLFALSILVFFI
                     TV"
BASE COUNT          510 a          297 c          314 g          451 t
ORIGIN      
        1 tggacggctt cgccgtcccc accccagcgc aggtaacgca aaccgttggg ctgtttaagt
       61 agagtgatac ggcatcttca tcaattcata ttcactgtca aataagagtt gtaagtaatg
      121 cgaccttttc tccaaaaata cagcatagca atttttattt tagtggcgtt aatcatcaca
      181 gttgggatgg ttgtttgggg caataatcct aacagcccag cgataatacg ggatctcggg
      241 ttaaatttag tttcagaagt gtttggtatc cttcttacgg tacttattat cgatgtaatg
      301 ataaaaagaa atgaagatag tagatggctc cccacgaaac atattactta tgccaaaatc
      361 accgaaatag cactcgaaat tcagtattac ttaaacacgg aagatgaaag aaaaaaacaa
      421 ccaatcgtag tgttctttgg cgtaactgac gccagaccag attaccatca atccgtttca
      481 atagaaaaat ttgaagaaat cgtaaaccaa taccataaac gagtcagaga aattattgac
      541 ctaacaggca tgttgttgga accagagata cagactcacc ttaatgctct tgagagaaac
      601 ttagaaaaaa cagcaggatt atttgtgaag tataaagatt tggataagtt gaagaatccc
      661 ctattgccaa taattaattt tgttgaagta aaagagagtg tagacaagat agtaagcctt
      721 atgctcgaaa agtcaacggg caaaatacca tttcatgaga gaacataaaa accaagtgct
      781 ttcaaaggct ttcatggttc ccagtcaaaa cagcccaaca aagcgtgcac ttgacgggtg
      841 ggattctgcg ccattttcgg gcatttttct acacccgaac agaatcctgc tctcaaagtt
      901 ttatctcgtc ccgcccaccc gcaagtaacg caagccgttg ggtgctggcg ctgaaaacaa
      961 aagaagttta cactggctaa caaagtcttt ctagcgggtt acttatggaa aaagcatcaa
     1021 taatattttc ggtgttgata cacagtgacg agttcatgta tttattttgg ggagcattgg
     1081 gagtaatcaa catgagttta ggcacttatt tattaaagtc aaaaggattg ggcgaaaaag
     1141 agctagcaga aatatcgcct aaattcaaaa acatcaattc agactatatg gtgtttagtc
     1201 tttttgctat tatgctcata attgcaccag tattgttatt gatgtcaggc gcagacaaat
     1261 gggcaatggg aaattacgga agaaggtttt atcctgctct tgttttcttt tgttcaggat
     1321 atggaattta tcaagcacta tttgctatat tcaaagatgt ttacccaatg ggaaaaatat
     1381 cgtattttat ttacgatgat aaaaaagtaa ttcgccgtgc cgcaaaaatt cacatcatat
     1441 cttcagtcgt tttgtttgct ctttctattt tggtcttttt tataacagtg taggtaagtc
     1501 caaaaggtgt acaattccaa aaacgcaccc aacaaagcgt gcacctgacg ctggggattc
     1561 tgcgcacatc cc
//
LOCUS       DAOQJE010000896         1564 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_74686_length_1564_cov_3.381710, whole genome shotgun sequence.
ACCESSION   DAOQJE010000896 DAOQJE010000000
VERSION     DAOQJE010000896.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1564)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1564)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1564
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_74686_length_1564_cov_3.381710"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            185..1471
                     /locus_tag="PLT08_17710"
     CDS             185..1471
                     /locus_tag="PLT08_17710"
                     /inference="COORDINATES: protein motif:HMM:NF013438.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 8"
                     /protein_id="HNE06368.1"
                     /translation="MKKLFLLSSALVIILGCMSAPVTTAPEEPTVQISLPTPTPEATL
                     ETLRRPFPQHVAYAPNSILPNHRTQEQLDSDVRTYYDYWKSTYVAEAGTNADGVLMYR
                     VVLGKDEPTRSTTVSEGQGYGMMIVPIMAGYDPDAQVIFDGMWAFVDAHRSEIDSRLM
                     DWNVPLEEGNASAFDGDADIAFGLLLADAQWGSNGHVNYKAEAEKMIAGILESTIGPE
                     SHLPMLGDWVNVGEPQYNQFTPRSSDFMLVNFRAFRNATNDPVWDDVVFQSQTLMAGV
                     QENYSPETGLMPDFIVNGAPAPENFLENVTDDAYNYNAGRVPWRVGADALLFDDAFSR
                     SLVQKISHWIEVSAEGDPMKIRSGYELSGAPLPNSDYFTTFFVAPMGVAAMNDPAQQE
                     WLNAIYSSVYANHIDYYEDSVNLLCMLVMSGNFWSP"
BASE COUNT          415 a          340 c          390 g          419 t
ORIGIN      
        1 aactgtagtg gcaggcttga gcgcgacttg acccgcgacg cgtacttgtc ccgccataat
       61 gagggcttgg gctttggcac gcgagtcagc cagtcctttt tctacaagga gaacatccaa
      121 acgaacttta ggcatggctc tattttactg ccagagatga tataaaagag aaaatggaga
      181 atgtatgaaa aaactttttc ttttaagttc tgctctcgtt attattttag ggtgcatgtc
      241 tgcacctgta acaacggcac ccgaagagcc tacggttcag atttctttac caacgccaac
      301 gccagaagca acccttgaaa cattgaggcg accttttcct caacatgtag cttatgcgcc
      361 aaatagcatt ttgccaaacc atcgcacaca agaacaattg gatagtgatg tgcgcactta
      421 ttatgattac tggaaatcga catacgttgc cgaagcaggg acaaacgcag atggtgtctt
      481 aatgtatcgt gtggtgttag gcaaggacga gcctactcgg tccaccactg tttcagaagg
      541 gcaaggatat ggcatgatga ttgtaccgat catggcagga tatgaccccg atgcgcaggt
      601 tatctttgac gggatgtggg catttgtgga cgcacaccgc agtgagattg actcacgttt
      661 gatggattgg aatgtgccat tggaagaagg aaatgccagc gcatttgacg gtgatgccga
      721 tatcgctttt ggtcttttgc ttgcagatgc acaatggggc agtaatggac atgtgaacta
      781 taaagctgaa gcagaaaaga tgatcgctgg gattcttgaa tccacgattg ggccggagag
      841 ccatttgccc atgttgggtg attgggtcaa tgtgggcgag cctcaataca atcaattcac
      901 gccgcgctcg tcagatttta tgttggtcaa ctttcgagct tttcgaaatg ccaccaacga
      961 tcctgtttgg gatgatgttg tttttcaaag ccaaactcta atggcaggtg ttcaagaaaa
     1021 ctatagccca gagactgggc tgatgccgga ctttatcgta aatggtgcgc ctgcccctga
     1081 aaattttctt gaaaatgtaa cggatgatgc ctataactac aatgctggac gcgtcccatg
     1141 gcgggtcgga gcggatgcgc tgctatttga tgatgcgttc tcacgttctc ttgtgcaaaa
     1201 aatttcgcat tggattgaag tcagcgcaga gggggatcca atgaaaatcc gttcggggta
     1261 tgaattgagt ggagcgcccc tgcctaacag tgattatttc accaccttct ttgttgctcc
     1321 aatgggagta gccgccatga acgacccagc gcaacaagaa tggctcaacg ccatttacag
     1381 ctcggtatat gccaaccaca ttgattatta tgaagactcg gtcaacttac tttgcatgtt
     1441 agtaatgagc gggaatttct ggtcgccata atttacaggt aaaataaaac catgttaact
     1501 gccttattca aaaccatgcg tccgcgccaa tggacgaaaa atgttttcat ttttgccgca
     1561 ttgg
//
LOCUS       DAOQJE010000897         1562 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_74842_length_1562_cov_6.016589, whole genome shotgun sequence.
ACCESSION   DAOQJE010000897 DAOQJE010000000
VERSION     DAOQJE010000897.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1562)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1562)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1562
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_74842_length_1562_cov_6.016589"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..461
                     /gene="msrA"
                     /locus_tag="PLT08_17715"
     CDS             <1..461
                     /gene="msrA"
                     /locus_tag="PLT08_17715"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: protein motif:HMM:NF013766.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HNE06369.1"
                     /translation="DSGYANGHVKNPSYRAVCNGDTGHAEVVQIKFDPSVITFRDLLN
                     IFFVIHDPTTLNRQGADVGTQYRSGIYYHMPEQKEVAEQTIRELEAQKVWGSRIVTEV
                     EPIKDFYIAEDYHQEYFVRNQSQPYCQAVVAPKVGKFRKHFLELWKKQAA"
     gene            576..>1562
                     /locus_tag="PLT08_17720"
     CDS             576..>1562
                     /locus_tag="PLT08_17720"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNE06370.1"
                     /translation="MNIYISLLQLRPKYRLLWLAQVISLAGDWFNTIASVIIVNRYTT
                     SGLALGGLFIARALPPFLLGPVAGVVADRFDRRKVLIASDILRAVIVLCFLLVDRPER
                     LWLMYVLTVLQFSVSTFFEPARAALTPALVETDELLNANTLASITWSAMLTLGGAIGG
                     VTASYFGVPVSLIVDAASFIVSAILVASITGEFRSQMAHALESGWKNFIDGMNYVRKN
                     LDVGFVTLVKGFGQVGSFDIIAALYAARLFPYGQEGATTLGLMFTAFGLGAVIGPLIA
                     NRFGDASANWLRRAILGGFVFMPLAWFIIGTATSFPIVLIGCVIRGMGGSINW"
BASE COUNT          339 a          361 c          419 g          443 t
ORIGIN      
        1 tggattcggg ctatgccaat gggcatgtga agaacccgag ttatcgcgcc gtgtgtaatg
       61 gtgatacggg tcatgcagaa gtggtgcaga tcaagtttga cccgtccgtc attacgtttc
      121 gcgacttgtt gaatatcttt tttgtcatcc acgacccgac cacattgaac cgtcagggtg
      181 cggatgtggg cacacaatac cgctcgggta tttactatca catgcccgaa caaaaggaag
      241 ttgccgagca gaccatccgc gagttggaag cgcaaaaggt ctggggcagc cgcatcgtca
      301 cggaagtgga gccgatcaag gatttctata ttgctgagga ttaccatcag gaatatttcg
      361 tgcggaatca atctcagcca tattgtcagg cagtggttgc accgaaggtt ggtaagttcc
      421 gcaaacactt cttggaattg tggaagaagc aagcagcgta gttcgtaagc atcatataaa
      481 aagggacgca gttgaatact gagctgcgtc cctttttatt tattcgagtt tcaactcgaa
      541 gtggaatgat tgttctcacg gctataattg cctgagtgaa tatctacatc tcccttctcc
      601 aacttcgtcc aaaatataga ttgctttggc tggcacaggt catcagcctt gcaggcgatt
      661 ggtttaacac catcgcttcg gtcatcattg ttaatcgcta tacaacatcc gggcttgcgc
      721 tcggagggtt attcattgcc cgtgcgcttc cgccattttt gctgggaccc gttgccggag
      781 ttgtagctga ccgctttgac cgacgcaaag tgctaatcgc aagtgatatt ctgcgagccg
      841 tgatcgtgct ttgctttctg ttagtagacc gacctgaacg gctatggctt atgtatgtgc
      901 tcaccgtctt gcaatttagt gtttctactt tttttgaacc ggcacgagca gcgctcaccc
      961 cggcattggt cgaaacggat gaacttctca atgccaatac cttggcgagt atcacttggt
     1021 ctgcgatgct tacgttgggt ggtgctatcg gtggggtaac agcttcttat tttggcgtgc
     1081 ctgtttcact cattgtggat gcggctagtt ttattgtttc agccatattg gttgccagca
     1141 tcaccggcga attccgttcg cagatggcgc atgcattgga gagcggctgg aaaaacttta
     1201 tcgacggcat gaactatgtt cgcaaaaatc tggatgtagg atttgtgacc cttgtgaaag
     1261 ggtttggaca agtaggcagc tttgacatta ttgctgcctt gtatgctgct cgcctctttc
     1321 cctacgggca ggagggagcg accacactag ggctgatgtt tacagccttt ggcttgggcg
     1381 cagtgatcgg tccgctcata gcgaatcgtt tcggcgacgc gagcgcaaat tggctgcgtc
     1441 gtgccattct tggcggattt gtattcatgc cgctggcatg gttcatcatc ggcacggcaa
     1501 catctttccc aattgtattg atcggatgcg tcatacgcgg gatgggtggt tcgatcaact
     1561 gg
//
LOCUS       DAOQJE010000898         1556 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_75475_length_1556_cov_4.702199, whole genome shotgun sequence.
ACCESSION   DAOQJE010000898 DAOQJE010000000
VERSION     DAOQJE010000898.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1556)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1556)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1556
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_75475_length_1556_cov_4.702199"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            186..671
                     /locus_tag="PLT08_17725"
     CDS             186..671
                     /locus_tag="PLT08_17725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE06371.1"
                     /translation="MDTSVFTQPISNLYQWTDVINRNLAYIKKELPKLSIPAEIRDEI
                     SAMCDGFQWMLSDVRKEIRNLEDKLGMHPGEEPFDPHITNPDPQLTIGLIQRWLLEEL
                     QTMHELIQKLDKLATQDSQSYDLVALLVRESATNILKAYLGTQNDLKFIVGQLEQHGG
                     K"
     gene            921..1499
                     /locus_tag="PLT08_17730"
     CDS             921..1499
                     /locus_tag="PLT08_17730"
                     /inference="COORDINATES: protein motif:HMM:NF024698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNE06372.1"
                     /translation="MNRRIPTLETERLIIRELTMDDLVSINNVLNKSFGWEMPLADRQ
                     RWLQWTVLGYEMFAILEQPHYGERAIVLKESGEIIGAVGIVPYLDTFNKVAAFKRELD
                     SPATAEVGLFWALDPAHHRKGYAPEAAYAVMEYLFVHEKIGRIIATTGYDNLPSQKVM
                     EKLGMKVQRLDEPQPPDQFVVGVIEKNDFQKN"
BASE COUNT          441 a          359 c          344 g          412 t
ORIGIN      
        1 agcgtgcacc tgacgctggg gattctgcgc acatctcaag cagtttcaca cgcctgagca
       61 tttttctggc tggacggctt cgccgtcccc gccccagcgc aggtaacgca atccgttggg
      121 cgctcagtca gtgagttacc tgttcaccct tggatcagac gcccacaggt cattgtagga
      181 gtattatgga cacatctgtc ttcacacagc ctatatcaaa cttatatcag tggaccgatg
      241 ttattaaccg caatctggct tatatcaaaa aagaattgcc aaaattatcg atcccggctg
      301 aaatccgaga tgagatttca gcaatgtgtg atggctttca atggatgctt tcggatgtca
      361 gaaaggaaat tcgcaatctc gaagataagc taggcatgca tcctggcgag gaaccatttg
      421 atccccatat taccaatccc gatccgcaac tgaccattgg tcttatccaa cggtggcttc
      481 ttgaagaatt gcagaccatg cacgaattga ttcagaaact tgacaagctt gccacacaag
      541 actcgcaatc ctatgacctt gttgctctcc ttgtcaggga atcagctacc aacattctga
      601 aggcatatct tggcacccaa aatgatctaa agtttattgt aggacagttg gagcaacacg
      661 gtggtaagta attttcttga aaggctacca tgggactcgt gccaaagaat tcttggaaat
      721 ccgacgtcca atctagtgca acaaaagcgc ccaacacagc gtgcacctga cgctggggac
      781 tctgcacaca tcccaggcag ttttctacgc cgtagctttt ttctggttgg acggcttcgc
      841 cgtccctgcc ccagcgcagg taacgcaatc cgttgggcaa ccccgtgcaa aataaagtaa
      901 aacttattca tttataacta atgaacagac gaattcctac tctcgaaact gaacgcttaa
      961 tcatccgtga attaacaatg gatgaccttg tttctattaa caacgtcctc aataaatctt
     1021 ttgggtggga aatgccgtta gcagatcgtc aacgctggct acaatggaca gtattgggtt
     1081 atgaaatgtt cgccattttg gaacagccac attacgggga aagagccatt gttctaaaag
     1141 aatcgggcga aattattggc gctgttggga tagttccata tttagatact ttcaataaag
     1201 tcgctgcttt caaacgcgaa cttgattctc ctgcaactgc tgaagtagga ttattttggg
     1261 cgttagaccc tgcacatcat agaaaaggat atgccccaga agctgcctat gccgtgatgg
     1321 aatatttatt tgttcatgaa aagattggac gaattattgc aaccacaggc tatgataact
     1381 tgccatctca aaaggtcatg gagaaattag gtatgaaagt acaacgactt gatgaacctc
     1441 agccacctga ccagtttgta gttggagtca ttgaaaagaa tgactttcag aagaattgat
     1501 ggtagagtct tttatcaatc atcgttttgg taagcaggca ggttgcccaa caaagc
//
LOCUS       DAOQJE010000899         1554 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_75679_length_1554_cov_4.611741, whole genome shotgun sequence.
ACCESSION   DAOQJE010000899 DAOQJE010000000
VERSION     DAOQJE010000899.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1554)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1554)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1554
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_75679_length_1554_cov_4.611741"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..235)
                     /locus_tag="PLT08_17735"
     CDS             complement(<1..235)
                     /locus_tag="PLT08_17735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE06373.1"
                     /translation="MQNEFKEDAPTPSGKPTSQRAGGTSNGPSLFTRLINSLVNMGLG
                     EPLIKLGVNLFSVVAIVLVVFMSRSFYRQAQDMP"
     gene            complement(300..>1554)
                     /locus_tag="PLT08_17740"
     CDS             complement(300..>1554)
                     /locus_tag="PLT08_17740"
                     /inference="COORDINATES: protein motif:HMM:NF017760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNE06374.1"
                     /translation="WLITRFPALASRDYVLFVVGQFISVIGTWMQATALPYLAYRISG
                     RPLDLGLIGFSNTLPTLLFALPAGVLVEHLDKRKAVILLQAVMSVQAFGLTYLAFTNK
                     LEIWHILVLAFIYGSAVSVEVTARQAMLIELAGKEALPSAIALQTTAFNLGRVIGPLL
                     AAWLIAISGNEGTVFLANGVSYVFVIIGLFFARTRYRVEPEAKTSQGMGSEFKEGVRY
                     IRSNTIVFSAILMSALLGFFGIPLVQQLPAIARDILQPLLTSEAAIATRTSNLYTAQG
                     IGAVTAAFMMAYFATANKNSTLLWGQILFIFPIIVLGLTTNASLAFFLLIFIGWGTVV
                     QLISMNTMIQMRVPNDLRGRVFSIYFWGLQGVAPFGSILIGWIAQTRDVQFAIVAGGI
                     ACLVGILVVRLLFFRKNEPTEQISY"
BASE COUNT          462 a          344 c          392 g          356 t
ORIGIN      
        1 cgggcatatc ttgcgcctga cggtaaaacg accgagacat aaagaccacc aacacaatgg
       61 caacaactga aaagagattt accccaagtt taattaatgg ctcgcctaac cccatattca
      121 caaggctgtt tataagtctt gtaaatagtg agggaccatt tgaggttccc ccagcgcgtt
      181 gagaggttgg ttttcctgag ggggtaggtg catcttcttt aaattcgttt tgcatagggt
      241 tcatgataac atggcaaaaa tttcacagtc aagttggggt gtaaaaccct catacagcct
      301 taataagaaa tttgctctgt aggctcattt tttcgaaaaa atagcaatcg cacgactaaa
      361 atgccaacta ggcaggcaat cccgcccgcc acaattgcaa attgtacatc tcgcgtttga
      421 gcgatccaac cgataagtat gctcccgaac ggagcgacgc cctgcagtcc ccaaaagtaa
      481 atgctaaaga cacgtccgcg cagatcgttg ggaacgcgca tttgaatcat ggtattcatg
      541 ctgataagct ggacgaccgt gccccaacca atgaagatca acaaaaagaa tgccagagag
      601 gcgtttgtgg ttaatcctaa tacgataatg gggaaaataa aaaggatttg cccccacagt
      661 aatgtggagt ttttatttgc agtcgcaaaa tacgccatca taaatgcggc agtcacggct
      721 ccgatgcctt gagcggtgta gagattgctg gtgcgggttg cgatggcggc ttcggaagtg
      781 agaagaggct gcaaaatatc acgtgcaata gcagggagtt gttgtacgag gggaataccg
      841 aaaaagccaa gcaatgccga catcaaaatg gcggagaata cgatggtgtt tgatcgaata
      901 taacgtacac cttctttgaa ttcactgccc atgccttgag atgtttttgc ttctggttca
      961 accctgtatc gtgttcgcgc aaagaacaga ccgatgataa cgaacacata actgacacca
     1021 tttgcgagaa agaccgtccc ttcgttgcca gagatggcaa tcaaccaagc ggctagtaac
     1081 ggtccaataa cacgcccaag gttgaaggcg gtggtttgca gggcgatggc acttggcagg
     1141 gcttcttttc ctgcgagttc gatcaacatg gcttggcgtg cagttacttc aacggagact
     1201 gctgaaccgt agataaacgc caacactaaa atatgccaaa tttcgagttt attggtaaag
     1261 gcaagatagg tcagaccgaa tgcttgcaca gacatcactg cttgcaggag gattactgct
     1321 ttacgtttgt ctaaatgttc aaccagtacg cccgcgggca aggcaaaaag cagagtaggt
     1381 aatgtgttgg agaaaccgat caaccccaag tctaatgggc gacccgagat ccgataggca
     1441 agatatggca aggcggtagc ttgcatccat gtgccgataa cggagataaa ttgacctacg
     1501 acgaatagaa cgtaatcacg tgaagcgaga gcggggaagc gggtaatgag ccag
//
LOCUS       DAOQJE010000900         1552 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_75849_length_1552_cov_6.990648, whole genome shotgun sequence.
ACCESSION   DAOQJE010000900 DAOQJE010000000
VERSION     DAOQJE010000900.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1552)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1552)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1552
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_75849_length_1552_cov_6.990648"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..467)
                     /locus_tag="PLT08_17745"
     CDS             complement(<1..467)
                     /locus_tag="PLT08_17745"
                     /inference="COORDINATES: protein motif:HMM:NF024853.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HNE06375.1"
                     /translation="MTKEMSKRQLRKEQIRRKEKQSRFLAIGLITVGALFLAFLVIYP
                     NLKPLGEVVTPKEITRANVDFNTAGDPNAPITITEYSDFQCPYCRIFFENTEELLMKQ
                     YVETGTVHFVYKSVGAFIGPESKAAAEAAYCAGDQGKFWEMHDMLFTNQTGENV"
     gene            complement(558..1397)
                     /locus_tag="PLT08_17750"
     CDS             complement(558..1397)
                     /locus_tag="PLT08_17750"
                     /inference="COORDINATES: protein motif:HMM:NF023568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipase"
                     /protein_id="HNE06376.1"
                     /translation="MKHQEFNWKAKDGLDIFAQVWEPDVVLPRAVVCLVHGLGEHSSR
                     YAHVAEAFSKEGYILFSFDHRGHGRSGGIRGHISSIEDFLQDIDQLLSQARTRYPNLP
                     VILYGHSLGGILVLNYSLNRKPDIKGVIATSSGLHTALEEQPVKIFMAKLLGSLMPKT
                     TLPSGLDAKAISRDETVVQTYNNDPLVHDKISLGFGKVMLGVTQWTLAHAGEFPLPLL
                     LLHGKSDTIAYPSSSTEFAAALKDRATLVLWDGAYHELHNEPEKAEVFKTMLMWMDAR
                     LRG"
BASE COUNT          430 a          374 c          326 g          422 t
ORIGIN      
        1 acattctcac ccgtttgatt ggtgaaaagc atatcgtgca tttcccagaa ttttccctgg
       61 tcgcccgcac aataagcagc ttcagctgcc gccttggatt cgggtccaat aaatgcacct
      121 acagacttat aaacaaagtg gaccgtcccg gtttccacat actgtttcat caataattct
      181 tctgtattct caaagaaaat acggcagtat ggacattgga agtcagaata ctcggtgatc
      241 gtaatcggcg cgttcgggtc accagcagta ttaaaatcaa cattggcgcg ggtaatttct
      301 tttggggtaa caacctctcc aagaggtttc aaattcgggt aaatcactaa aaatgcaaga
      361 aatagcgcac ccacggtgat caaaccaatc gctaaaaagc gggactgttt ctctttgcga
      421 cggatctgct ccttgcgtag ttggcgttta ctcatctctt tcgtcatgct agctcctatt
      481 atttttttat cggcattgca ttttctcatg ccaaaataga tttgtccaga aatgggttaa
      541 caggttctta ttttcgctta gccccgcaag cgggcatcca tccacatgag catggttttg
      601 aaaacttctg ccttttctgg ctcgttatga agttcatggt aagcaccatc ccaaagaaca
      661 agcgtagctc ggtcttttag ggcagcggca aactcagtac tgctggaagg ataagcgatt
      721 gtgtcgctct taccatgcaa caataacaaa ggaagcggga attcgccagc gtgcgccaga
      781 gtccattgag tcactccaag cataaccttc ccaaacccga gtgaaatctt gtcatgaaca
      841 agcgggtcat tattgtaggt ttgcacaact gtttcatcac gagagatggc ttttgcatcc
      901 aaaccactag gaagggttgt cttgggcatc agagatccaa gcaattttgc cataaatatc
      961 tttacaggtt gttcttccaa agcggtatga agcccagaac tggtagcaat gacgccctta
     1021 atatccggct tgcgattcaa actgtagttc aagactagga taccacctaa gctatgccca
     1081 tacagaataa caggcaggtt cgggtatcga gtccttgctt gagataataa ctgatctata
     1141 tcttgtagga agtcttcaat agaagagata tgcccacgta taccgccaga gcgaccatgt
     1201 ccgcggtgat cgaaggagaa gagaatgtat ccttctttgc taaatgcttc ggcaacatga
     1261 gcataccgtg aactatgttc acccaagccg tgcactaagc acaccacggc acggggtaac
     1321 accacatctg gctcccaaac ctgggcgaaa atatcaagcc cgtcttttgc tttccaatta
     1381 aattcttgat gtttcatgtc tatgatgctc caatcgtgat cttaaattca cattgatgag
     1441 accccgccgc tttacagaca agttcttcaa tgtcatgttc atcgccagtt gcccaatata
     1501 aacattcacg gatcaagccc agggttacaa aacacacagg ggaattttct tt
//
LOCUS       DAOQJE010000901         1551 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_75942_length_1551_cov_4.763369, whole genome shotgun sequence.
ACCESSION   DAOQJE010000901 DAOQJE010000000
VERSION     DAOQJE010000901.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1551)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1551)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1551
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_75942_length_1551_cov_4.763369"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(59..679)
                     /locus_tag="PLT08_17755"
     CDS             complement(59..679)
                     /locus_tag="PLT08_17755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE06377.1"
                     /translation="MKLSFKTITSFPLNLLLILAVASGLISCSQQPVLASSSQNEQLP
                     FVATPTAESQPTQQSENFETTYGITVTAKALCTDGTNTTLYLETNLDSSLWQLNEKDF
                     YPLGKTYFETSILFLENGEMFSMYSSGKRDDPIFNSQNTIVSTIQTFIFPKIPSANST
                     FTIKAKVSLHDLPATYAPPVKMDFLEPGIIEVPMEYVASATIAECP"
     gene            complement(874..>1551)
                     /locus_tag="PLT08_17760"
     CDS             complement(874..>1551)
                     /locus_tag="PLT08_17760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE06378.1"
                     /translation="GRLRRPRPSAGNANRWAARHINMTEQTKTTLMLCLISAIFCGFL
                     LAGEDCGVSNASENLRGECHIMAMNIPTKLQTHWINWVPGILFGIAFALGNSFNKFGI
                     VAFSLVSGIIYYIAGLIFALFGIQLNFDEKLSWIVAVFATGSVFAGVFGATSLLFLVK
                     YLSKAQIVFGIEIRMAGVGALAGIIFFFCGLLGLLAFPFWLLAFAIWQVFIGFYLTTF
                     LQKGSIA"
BASE COUNT          486 a          307 c          321 g          437 t
ORIGIN      
        1 ttgggatgtg cgcagaatcc ccagcgtcag gtgcacgctt tgttgggcgg ttttgctgtt
       61 atggacactc tgcaatggta gcggatgcaa catactccat tggcacttcg ataattccag
      121 gctctaagaa atccatttta actggaggag cgtaggtcgc gggtaaatca tgtagagata
      181 cttttgcttt gatagtaaat gtggaattgg cagaaggtat ctttggaaaa ataaaggttt
      241 gtatcgtgct gactatggta ttttgtgaat tgaaaattgg gtcatcacgt ttcccgctcg
      301 aatacattga aaacatttcg ccattttcca agaataaaat gcttgtctca aagtaagttt
      361 ttccgagagg ataaaagtct ttttcgttca attgccagag actcgaatcg agattagttt
      421 ctaaataaag tgtagtgttt gtgccatctg tgcataatgc ttttgctgtg actgtaatgc
      481 catacgtggt ttcaaaattt tcagattgtt gagttggttg actctctgct gttggagtag
      541 caacaaaggg caattgttca ttttgagatg aactcgccaa tactggctgc tgtgaacagg
      601 aaataagtcc acttgctaca gccaatatca acaacaaatt aagaggaaaa cttgtaattg
      661 ttttgaatga tagtttcatt tttctccaca aattaaattt atccgaatta ggggtgaact
      721 attttgccac gaaccgccca acggcttgac attacctacg ctggggcagt ggacggcgaa
      781 gccgtccaac cagaaaaatg ctaaggcgtg tgaaactgcc tgagattgcc gcagaatccc
      841 cagcgtcagg tgcacgcttt gttgggcagc attttatgca atagaacctt tctgaagaaa
      901 agttgttaag taaaatccga taaatacttg ccatattgca aaagcaagga gccaaaatgg
      961 aaacgcaagt aagcctagta aaccacagaa aaagaaaata ataccagcta atgcgccaac
     1021 tccagccatt ctaatttcaa tcccgaaaac tatttgagct tttgatagat atttaacaag
     1081 aaataataaa gaagttgctc caaacactcc tgcaaaaaca gaacctgttg caaatacagc
     1141 tactatccat gataacttct catcaaaatt aagttgtatt ccgaacaatg caaagattaa
     1201 tccagcaatg taatagatta ttcctgaaac taatgagaac gcgacaatac caaacttatt
     1261 gaaagaattt cctaaagcaa atgcgatgcc aaataaaata cctggaaccc aatttatcca
     1321 atgagtctgc aatttcgtgg gaatgttcat agccataatg tgacattccc ctctaagatt
     1381 ctcacttgcg ttagagactc cacaatcttc accagctaga aggaaaccac agaaaatcgc
     1441 agaaattaga cataacatca atgttgtttt tgtttgctca gtcatgttga tatgccttgc
     1501 tgcccaacgg tttgcgttac ctgcgctggg gcggggacgg cgaagccgtc c
//
LOCUS       DAOQJE010000902         1546 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_76431_length_1546_cov_8.374245, whole genome shotgun sequence.
ACCESSION   DAOQJE010000902 DAOQJE010000000
VERSION     DAOQJE010000902.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1546)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1546)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1546
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_76431_length_1546_cov_8.374245"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..930
                     /locus_tag="PLT08_17765"
     CDS             <1..930
                     /locus_tag="PLT08_17765"
                     /inference="COORDINATES: protein motif:HMM:NF012382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HNE06379.1"
                     /translation="THPENVLITAGSQQAIFLASQVLLKPNDIVLVEDPTYSAALDLF
                     RTLGFQIVGIPVDSQGMQVEKLEKLLQQYHPKLIYTIPNFHNPTGTCLSSARRRELIL
                     LAGKYNVPILEDDFVGDLRYEGHTQPSLKSLDPGGQVIYISTFSKMLMPGLRVGFIVA
                     DGPVYDSLLNFKRLSDLATSTLIQRALDAYMTVGRYQTYLHRSNQIFRSRRDAMLDAI
                     KKHLPSTINFELPKGGLFIWLQLPKSISANELLPIACKEGVAFSPGNSFFIDGISGED
                     WIRLNFASQPVEDIEEGIKRLGNALKKMKPKKK"
     gene            1055..1477
                     /locus_tag="PLT08_17770"
     CDS             1055..1477
                     /locus_tag="PLT08_17770"
                     /inference="COORDINATES: protein motif:HMM:NF012610.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HNE06380.1"
                     /translation="MTNTKLTLHLDFHSGLPIYTQIVNQIQSQLANGILKPGDQLPTV
                     RALASELRVNFNTVARAYRILDEARIISTQQGRGTFITEIPPPEVTEKLRQESLSELT
                     NRFLSEAFRLGFSEREVSQMVRDRLKSWKEAKQVKEEE"
BASE COUNT          466 a          355 c          322 g          403 t
ORIGIN      
        1 actcatcccg aaaatgttct catcactgct ggctcacaac aagcaatctt tctcgcatca
       61 caagtcttgt tgaagcccaa tgacattgtt ctcgttgaag accctacata ctctgcagca
      121 ttggacctct tccgcacatt aggttttcag atagtcggta ttcctgtcga tagccaaggc
      181 atgcaagtag agaaactaga aaaacttcta cagcaatatc atcccaaact gatttacacg
      241 attccgaatt tccacaaccc aactggcact tgtctcagca gtgcccgccg ccgcgagttg
      301 attctattgg caggcaaata caatgtgccc attctagaag atgatttcgt aggtgacttg
      361 cgctatgaag gtcacacaca accttcgctc aagtcattag acccaggtgg gcaagtcatc
      421 tacatcagca ccttttcgaa aatgctgatg cccggtctgc gcgtcggttt catcgtcgca
      481 gatggacctg tctacgatag tttgctcaat ttcaaacgtc tcagcgactt ggcaacttcc
      541 acgctgattc aacgtgcgct cgatgcctat atgacggtgg ggcgttatca aacctatttg
      601 catcgctcca atcaaatctt ccgttcacgg cgagatgcga tgttggatgc catcaaaaaa
      661 catttgccaa gcactattaa ttttgagcta ccgaaaggcg gtttatttat ttggctgcaa
      721 ttgcccaagt ccatttcggc gaatgaattg cttccaattg cttgcaagga aggtgtcgca
      781 ttttcgcccg gcaattcatt ctttattgac ggcatcagtg gagaggattg gatccgcctc
      841 aactttgcat cgcaacctgt agaagatata gaagagggga ttaagcgatt gggaaacgcc
      901 ttgaaaaaga tgaagccaaa gaaaaaataa atctgtgcga aaaggacttt gagaatttcc
      961 tcaaagtcct tttttattct taaggggctt gacttaaagt ccttaagtgc tacaattcaa
     1021 tcattgatac aattagtaca atttaaaaac ctctatgaca aacaccaaac tcaccttaca
     1081 tctcgatttt cattccggct taccaatcta tacccagatc gtgaatcaaa tacaaagcca
     1141 actggcaaac ggtattttga aacctgggga tcaattacca accgtgcgcg cattggcatc
     1201 tgaattgaga gtcaacttca atacagtagc acgtgcttac cgcatactgg atgaagcacg
     1261 aatcatatcc acacaacaag gacgcggcac gttcatcact gagattccgc caccagaagt
     1321 gacagagaag ttacggcaag agtcactctc tgaacttacg aatcgatttc tcagtgaagc
     1381 atttcggttg ggtttctctg aacgtgaagt aagccaaatg gtgagagacc gtttgaagtc
     1441 gtggaaagaa gcgaagcaag tcaaagagga agaataaagt ttcaagttag gagaatgcac
     1501 atatgaatac aaagtttagt aagttgatga atacaaagaa aaaaga
//
LOCUS       DAOQJE010000903         1545 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_76580_length_1545_cov_2.529530, whole genome shotgun sequence.
ACCESSION   DAOQJE010000903 DAOQJE010000000
VERSION     DAOQJE010000903.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1545)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1545)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1545
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_76580_length_1545_cov_2.529530"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..834
                     /locus_tag="PLT08_17775"
     CDS             <1..834
                     /locus_tag="PLT08_17775"
                     /EC_number="2.8.4.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008172 - S-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MiaB/RimO family radical SAM
                     methylthiotransferase"
                     /protein_id="HNE06381.1"
                     /translation="CTFCVTTIARGEGRSRPLADVINDVNSALAGGSKEIVLTGVHLG
                     SWGQDFGNHLRELVKALLRETDVQRLRLSSLEPWDLDTDFFSLWNDKRLMPHLHLPLQ
                     SGSDSTLKRMLRKTTTASFRELVHAARSILPDVAITTDIIAGFPGETDEEFAETMEFV
                     QEMRFTGGHIFSYSPRPGTGAAKMKGQVKPEVRKRRNRILQEVIEESAKSYREKFIGQ
                     TMRVLWESTTEYGEYGWRMEGWTGNYLRVSATAPSPRWNEVDAVTLCRIKSGILEGDI
                     V"
     gene            831..1391
                     /locus_tag="PLT08_17780"
     CDS             831..1391
                     /locus_tag="PLT08_17780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE06382.1"
                     /translation="MTKFKPTYALIILIISNLFLGFLIGNQYGQTWDEPSFYLYGERT
                     YDAYLRGITGEPLNPEKNIYFLDLRYYGPVYTATGWRMVEALRPWLKTWGDIDIWHLI
                     NYCFFQIALVSLFFLSKRFVRPWVAFFIVLLFNTQPLIFGHAFINPKDIPFLTFFLAS
                     ITAGLYMVDKIKETEDLGNQPFTLIL"
BASE COUNT          394 a          314 c          359 g          478 t
ORIGIN      
        1 tgtacatttt gtgttaccac tattgcgcgc ggggaggggc gttcgcgccc attggcagat
       61 gtgatcaacg acgtcaactc ggcattggct ggcggttcga aagaaattgt tctaacaggc
      121 gttcatcttg gctcatgggg gcaggatttt ggcaaccatc tccgtgagct agtcaaagct
      181 cttttgcgcg aaacggatgt tcaacgcctg cgcttatcct ccctagaacc ttgggatctg
      241 gatacagatt tcttctcgct ttggaatgac aaacgcctca tgcctcacct gcacctgcct
      301 ctgcaatctg gcagtgactc taccctgaag cggatgctgc gcaaaaccac aacagcttcg
      361 ttccgtgagt tagttcatgc ggctcgaagt atcttgcctg atgtggcgat taccacagac
      421 attatcgctg gtttccccgg cgagacagat gaagaatttg cagagacgat ggagtttgtt
      481 caagaaatgc gtttcacggg cggacatatc ttctcctatt caccacgtcc cggtactggg
      541 gcggcgaaga tgaagggaca ggtcaagccc gaggtccgca agaggcgtaa tcgtattttg
      601 caagaagtga tcgaagaatc ggcaaagtcg tatcgtgaaa agtttatcgg tcaaaccatg
      661 cgcgtgttgt gggaatccac aactgaatat ggtgaatacg gctggcgcat ggaaggctgg
      721 acgggtaatt atctacgtgt gagcgctact gccccttctc cgcgttggaa tgaagtggat
      781 gcggtgacgt tatgtcgcat caaaagtggg attcttgaag gagatattgt gtgacaaagt
      841 ttaagccaac ttatgcatta ataattctta ttatctcgaa cttgttttta ggatttttga
      901 ttggcaatca gtacgggcaa acatgggacg aaccatcatt ttatttgtat ggtgaacgaa
      961 cctatgacgc ttatttacgc ggaattactg gtgaaccctt aaatcctgaa aaaaatatat
     1021 actttttgga tctacgctat tacggtccag tatatactgc aactggttgg cggatggttg
     1081 aagcattaag accttggttg aaaacttggg gagatatcga tatttggcat ctgataaatt
     1141 attgtttttt tcagatagcc cttgtttctc ttttttttct ttcaaaacgt tttgtccgcc
     1201 catgggttgc attttttata gtgctcttat ttaacactca accgttaatc tttgggcatg
     1261 catttataaa ccctaaagat attccgtttt tgactttttt tctcgccagt ataacggctg
     1321 ggttgtatat ggtggataaa ataaaagaaa ctgaagattt aggaaatcaa ccttttactt
     1381 taattttata actttcaatc atatttgctg tttatgtatt cacttttatt ggaaaagact
     1441 tgatttctgg gctgattggc agcgtaatag cagctttcta taatgcgcca gaaagctctt
     1501 ttctagcgaa attattttta ctcatatctg gaaactcaca accac
//
LOCUS       DAOQJE010000904         1538 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_77224_length_1538_cov_3.525287, whole genome shotgun sequence.
ACCESSION   DAOQJE010000904 DAOQJE010000000
VERSION     DAOQJE010000904.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1538)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1538)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1538
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_77224_length_1538_cov_3.525287"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            120..914
                     /locus_tag="PLT08_17785"
     CDS             120..914
                     /locus_tag="PLT08_17785"
                     /inference="COORDINATES: protein motif:HMM:NF013964.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HNE06383.1"
                     /translation="MQYKTRQFIFHRTYEMASLVEACVVDSSSFIEIDWEKPEVLRCL
                     VEFSKVSLLHYYIYSMISANERQNLFDRDDIYEDSEEEKESIEVAFRAYSIDFLPYSK
                     FLLENTGDEGGFLFRKWFNSQQDKFELLWGKMTDDVFHLLYANRAFLLQFNQSLAKYL
                     QSGEVNLPTKYLDKKGVLKRQSYFPSWLKKAVFYRDQGKCVLCQRDLSDLLNTDQRIH
                     FDHIVPLNLWGSNDPSNLQLLCEECNLKKSGTSSKTATRYNPWWSS"
     gene            1107..1472
                     /locus_tag="PLT08_17790"
     CDS             1107..1472
                     /locus_tag="PLT08_17790"
                     /inference="COORDINATES: protein motif:HMM:NF013098.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HNE06384.1"
                     /translation="MATFRYLVHDVESAIHFYTTHLGFKLIDQMGPAFAIVAKEDLNL
                     WLSGPQTSAARLMPDGRKPEAGGWNRFVIEVENIESLVEEMKQTGVSFRNEIISGPGG
                     KQILVEDPSGNPIEIFQPA"
BASE COUNT          469 a          317 c          314 g          438 t
ORIGIN      
        1 gaatacatca agctttattt ctcgctggac ggatgaggca agtctacgca acggggcagg
       61 tatctcctat cagggacggg cagcaaacca ttaggcggct ggcgcaataa gaaaaacaca
      121 tgcaatataa aactcgtcaa tttatcttcc acagaactta cgaaatggct tcattggttg
      181 aagcctgcgt tgtagactcg tcatcattta tagaaattga ttgggaaaaa cctgaagtct
      241 taagatgttt agtggaattt tccaaggtaa gtcttcttca ttactatatt tactcgatga
      301 tttcagctaa tgagcggcag aatctttttg acagagatga tatttatgaa gatagcgaag
      361 aagaaaaaga aagtatagaa gttgcctttc gtgcgtatag catagatttt ctgccatatt
      421 caaaattttt attggaaaat acaggcgacg aaggcggttt tctttttcgt aagtggttta
      481 attctcaaca agataagttt gaattgcttt ggggaaaaat gacagatgat gtttttcatc
      541 tcctgtatgc aaatcgagca tttttattac aatttaatca gtctcttgct aaatatcttc
      601 aatcaggaga agtaaatctt cctaccaagt atcttgataa aaagggagtt ctaaaacggc
      661 agtcatattt tccatcttgg ctaaagaagg ctgttttcta tcgtgaccaa gggaaatgtg
      721 tcttatgcca acgtgacctt tcagacttgc taaatacaga ccaacgcata cactttgacc
      781 atatagtgcc gctcaatctt tggggtagca atgaccccag taatttgcaa ttactttgcg
      841 aagaatgtaa tctgaaaaaa tctggaacgt cttccaaaac cgcaactcgg tataatcctt
      901 ggtggagttc ctgaaaaaca caggacaaca aagcgtgcac atgacgctaa ggactctgca
      961 gcaatctcaa gcagttttct gtgccttaac cttcttctgg ttgaatagct ttatcaacat
     1021 cacatcaaaa cttacataca ttattttatt gcccaaacga acctatgtat aatcccctac
     1081 acatccttta gaagtctgga gacactatgg caacttttcg ttatctggta catgatgttg
     1141 aatccgcgat ccatttctac accacccacc ttggcttcaa actcattgat caaatgggtc
     1201 ctgccttcgc gatcgtagcg aaagaagatt taaacctatg gctgagtggt ccgcagacct
     1261 ctgcagcgcg cctcatgccc gatggcagaa agccagaagc cggtggctgg aaccgttttg
     1321 tgatcgaagt agaaaacatt gagtctctgg tagaagaaat gaagcagact ggcgtatcgt
     1381 ttcgaaatga gattatttct ggtcctggtg ggaaacagat ccttgtcgaa gacccgtctg
     1441 gcaacccgat agagatcttt caacccgcat aataagcatg gtattgtata aaaccttagg
     1501 agtatccaca tgacgaccct tactcaaacg accgaaaa
//
LOCUS       DAOQJE010000905         1526 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_78461_length_1526_cov_5.123725, whole genome shotgun sequence.
ACCESSION   DAOQJE010000905 DAOQJE010000000
VERSION     DAOQJE010000905.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1526)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1526)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1526
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_78461_length_1526_cov_5.123725"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1526)
                     /locus_tag="PLT08_17795"
     CDS             complement(<1..>1526)
                     /locus_tag="PLT08_17795"
                     /inference="COORDINATES: protein motif:HMM:NF013584.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HNE06385.1"
                     /translation="FESAGLTWLRKQQIEEAPTYLPIHYVGELQKKDAAIPEAQWPVT
                     PSQTRGFQFASIFDFAKAYRAGTTTPEDVAKKVLDAIEASDKSDAPLRAFIAVNRDEV
                     MRQARESTERFKAGKPLSIFDGVPVAIKDEMDVTPYPTTVGTSFLGKSPAKEDSEVAA
                     RLRKAGAMLVGKTNMHEIGINVFGLNPHHGTTRNPYNTGHFTGGSSSGSATAVAAGLV
                     PVAIGADGGGSIRIPAAFCGLVGLKATFGRVSEYGAFPLDWSVAHIGPIAGTATDVAL
                     TYALLAGPDLKDEVSLHQPLPTLEGWDNINLKGLKIGIYRPWFEHAEKEVVDACTAML
                     KKFEEMGCEIKEIVIPNLEANRVAHTVTIVSEMVQGMSATYDQHHKEHGLDVRINLAL
                     GQKFSADDYVKAQRIRTRMIRHFNNVFKEVDVILTPTTAIAAPEIKKGALPDGDSDLS
                     TTVEIMRFATAGNLTGLPAITFPVGYTQKGLPIGLQAMGRAWDEVTLLRLALASEKVI
                     "
BASE COUNT          346 a          389 c          384 g          407 t
ORIGIN      
        1 tcaataactt tttccgaagc taaagccaac cgtagtagag taacttcatc ccatgctcgt
       61 cccatagctt gtagcccaat cggtaaaccc ttttgtgtat aaccaaccgg gaaggtaata
      121 gcaggtaagc cggttaaatt cccggcggtg gcgaagcgca tgatttccac agtggtgctc
      181 aagtcagaat cgccatcggg tagagcacct tttttgatct ctggagcggc aatggcagtg
      241 gtgggggtca gaatgacatc cacttctttg aatacattat taaagtgacg gatcatccgt
      301 gtccgaatac gctgcgcttt gacataatcg tctgcgctga atttttgccc gagtgcaaga
      361 ttgatgcgta catccaaccc gtgttctttg tggtgttggt cataggttgc gctcatccct
      421 tgcaccatct cactgacgat ggtcactgta tgggcaactc ggtttgcttc aaggttcggg
      481 atgacgatct ctttgatctc acagcccatc tcttcaaatt ttttgagcat ggcagtacag
      541 gcatctacaa cttctttttc tgcatgttca aaccatggac gataaatacc gattttgagc
      601 cccttgaggt tgatattatc ccaaccctct aaggtgggca aaggttggtg gagagaaact
      661 tcatctttca ggtcgggtcc tgccagaagg gcataggtca atgcgacatc ggtggctgtg
      721 ccggcaatgg gaccaatatg cgccacgctc caatccaacg gaaatgcgcc atattcgctc
      781 acacgcccaa atgtggcttt caagccgacc aagccgcaaa acgccgccgg aatacgaatc
      841 gaaccgcctc catcggcacc gatggcgacg ggcaccagtc ctgcagcaac agcggtggca
      901 gagccgcttg aacttcctcc tgtaaagtga ccggtgttgt atgggttgcg ggttgtgccg
      961 tgatgagggt tcaacccaaa gacattgatg ccgatttcat gcatgtttgt cttgccgacc
     1021 aacatggcac ccgctttgcg taaacgtgcc gccacttccg aatcttcttt ggctggagat
     1081 ttccctaaga aggatgtgcc gactgtcgtt ggatacggtg ttacatccat ttcatctttg
     1141 atcgcaaccg gcacgccatc gaagatgctg agcggtttgc cagccttgaa acgttccgtc
     1201 gactcgcggg cttgacgcat aacttcatcg cggttcacgg cgatgaatgc tcgtagcggc
     1261 gcatcagact tgtcactggc ttcaatggca tctaagacct tttttgccac gtcttcgggt
     1321 gttgtcgttc cggcacgata ggctttggca aaatcgaaaa tgctcgcaaa ttgaaagcca
     1381 cgtgtttgag atggtgttac gggccactgt gcttcgggaa ttgccgcgtc ttttttttgt
     1441 aattcaccca catagtggat cggcagataa gtaggtgctt cttcaatctg ttgtttacgt
     1501 agccaagtca acccggcgct ttcaaa
//
LOCUS       DAOQJE010000906         1520 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_79065_length_1520_cov_8.774744, whole genome shotgun sequence.
ACCESSION   DAOQJE010000906 DAOQJE010000000
VERSION     DAOQJE010000906.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1520)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1520)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1520
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_79065_length_1520_cov_8.774744"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..380)
                     /locus_tag="PLT08_17800"
     CDS             complement(<1..380)
                     /locus_tag="PLT08_17800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNE06386.1"
                     /translation="MTEMDRRRILVVDDEERMVRFIRMNLEHDGFQVGEAFNGKQAIQ
                     KIRDFTPDLILLDVMMPDIDGFEVLETIRDFSNVPVIMLTAKGEEDDRVRGLEHGADD
                     YITKPFSPRELVSRIKAVLRRTEGT"
     gene            complement(385..627)
                     /locus_tag="PLT08_17805"
     CDS             complement(385..627)
                     /locus_tag="PLT08_17805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE06387.1"
                     /translation="MAYTVILHIANEASVAGEVDELPKPTDTIIVVANPRQKDGKDLH
                     YIDQGTVKVIWPLAKVSFVEILGDSEEEKIIGFVRE"
     gene            complement(649..1155)
                     /locus_tag="PLT08_17810"
     CDS             complement(649..1155)
                     /locus_tag="PLT08_17810"
                     /inference="COORDINATES: protein motif:HMM:NF012708.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HNE06388.1"
                     /translation="MIICSNCKHANMTGAMFCVECGAQLIGRDTLTTQNIATDSLGRA
                     SKRKTGELSQPIEGVDAWANLQLLDTGQVLPLSSRNEFTLGRISEGQPIMPDIDLSPY
                     QAYAAGVSRLHAVIKRSGSYVKFMDLGSANGTFLNGIRLSPNVEQSIQHGDIVSLGKM
                     RIQILIEK"
     gene            complement(1152..>1520)
                     /locus_tag="PLT08_17815"
     CDS             complement(1152..>1520)
                     /locus_tag="PLT08_17815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE06389.1"
                     /translation="SIASNPIPASPIRLKLTRPLEQSPSAEPPPTRVLKALSRLTLYR
                     MQERAQSAAQNGQFDVAARHLQNLATHLLSQGEHAVAKTALLEAQSLENTRTWSESGS
                     KDIKYKTRALLLDSGKEKKS"
BASE COUNT          389 a          379 c          323 g          429 t
ORIGIN      
        1 gtgccttcgg tgcggcgcag tacggcttta atacggctga ccaattcacg cgggctgaac
       61 ggtttggtaa tgtaatcgtc cgcgccatgt tcgagtccgc gcacacggtc atcctcttca
      121 cctttggcgg tgagcataat gaccggcaca ttactaaaat cgcggatggt ctcaagcact
      181 tcgaagccgt caatatccgg catcatcaca tcaagcaaaa taaggtcggg agtgaaatca
      241 cgaatctttt ggattgcttg cttgccgtta aacgcctccc ctacctgaaa accatcatgt
      301 tccaggttca tacgaataaa gcgcaccata cgctcttcat catccacaac caggatgcga
      361 cgtcgatcca tttctgtcat acgtctattc cctcacaaaa ccaataatct tctcttcttc
      421 agagtcacca aggatttcga caaaacttac tttcgctaaa ggccaaatca ccttaacggt
      481 tccttgatcg atatagtgca agtctttgcc atctttctgg cggggattgg caacaacaat
      541 aatcgtatcg gttggtttag gaagctcatc cacttcacca gccacagatg cttcattagc
      601 aatatgcaaa ataacggtgt aagccatatc cgttgtctct ccttcgttct atttctcaat
      661 taatatttga attctcatct tacccaatga cacaatatca ccgtgctgaa tcgattgctc
      721 cacatttggt gataaacgaa tgccatttag aaaggttccg tttgcagaac ccaagtccat
      781 aaatttgaca taagagccgc ttcttttgat gacagcatgt aagcgtgaaa ccccagcagc
      841 atatgcctgg taaggagaga gatcaatatc cggcataatt ggttgcccct cactaattct
      901 ccctaacgtg aattcatttc gagaagagag aggtaacact tgcccagtat ctaaaagttg
      961 tagattcgcc caagcatcaa caccttctat tggctggctc aattcaccgg tttttcgttt
     1021 gcttgctctt ccaaggctat ctgtcgcaat attctgggtg gttagtgtgt ctcttccgat
     1081 caactgggcg ccacattcca cacagaacat ggcacctgtc atatttgcat gtttgcaatt
     1141 ggaacagatt atcatgattt tttctccttg ccactgtcca ataacagcgc tcgtgtttta
     1201 tattttatat ccttgctccc agattcgctc caggtacgcg tattttcaag actttgtgct
     1261 tcaagcaatg cagtttttgc aacagcatgt tcgccttgtg aaagcaaatg cgttgctaaa
     1321 ttttgcaaat gcctggctgc tacatcaaac tgtccattct gggcagcaga ttgcgcccgt
     1381 tcttgcatgc gatacagagt aaggcgggaa agcgccttca aaacccgcgt cggcggtggc
     1441 tctgctgagg gactttgctc caagggacgg gtcagtttga ggcggatggg agatgcaggg
     1501 ataggattgg aagcaataga
//
LOCUS       DAOQJE010000907         1518 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_79295_length_1518_cov_2.872180, whole genome shotgun sequence.
ACCESSION   DAOQJE010000907 DAOQJE010000000
VERSION     DAOQJE010000907.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1518)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1518)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1518
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_79295_length_1518_cov_2.872180"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            72..740
                     /locus_tag="PLT08_17820"
     CDS             72..740
                     /locus_tag="PLT08_17820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE06390.1"
                     /translation="MPPTKKHPQNTEKIKLIVEIAVAVISVMSLFFAWQANQISKKTI
                     QPQVLVLDTFPIEFETWANGNLETAVCRTLVRLANVGGVDTDLFTASVKADTGTTSSV
                     VPMSEIITSIRTETLEEDVLFFASIWRPSPDDTRDPAVIWKDGLYYDLPISLKSHTTS
                     NVYVDTYFWYVLDKYDFHTISLTPVDEAFSPVGYKPINVSFIFQFPDESTITTNPTQC
                     AYFK"
     gene            937..>1518
                     /locus_tag="PLT08_17825"
     CDS             937..>1518
                     /locus_tag="PLT08_17825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE06391.1"
                     /translation="MAKDLGFSFEKASNSNYKKLLNISLWANRFSWVILFLYTIDFLA
                     RTAFQIQEIFGEWSIKLSISFLANLLAIPVVGIIYFLILQTISGGILILMDNNNLELI
                     RTLNVKRNNAIQFIPHWANALSWVVLSLNTIYFLVNLVLYYPLIFEKDSGYELGYMNE
                     LGMCTQLFITLVIGIVYFLILQTIASSILIFKEK"
BASE COUNT          467 a          295 c          294 g          462 t
ORIGIN      
        1 cggcttcgcc gtccccgccc cagcgcaggt aacgcaaacc gttagcccgc cccttgcata
       61 atagagaaaa tatgccccct acaaaaaaac atcctcaaaa cacagaaaag attaaactca
      121 ttgtagaaat agcggttgca gtcattagtg ttatgagtct attctttgct tggcaagcaa
      181 atcaaataag caaaaagact atccaaccgc aagttctagt tttagacaca tttcccattg
      241 agtttgaaac atgggcgaat ggaaatcttg aaaccgctgt gtgcagaact cttgtgaggc
      301 ttgcaaatgt tggcggtgtt gatacagatt tatttacagc atcagtaaaa gccgatacag
      361 gaactacttc cagtgtcgtg ccaatgagtg aaatcatcac atcaatacga acagagacac
      421 ttgaagaaga tgtactgttt ttcgcttcga tatggagacc ttccccagat gacaccagag
      481 acccagcggt gatttggaaa gatgggttgt attacgattt accaatatca ttgaaaagcc
      541 acactacatc taatgtatat gtggatacct atttttggta tgtacttgat aagtatgatt
      601 ttcatacaat aagtttaacc ccagtcgatg aagcattttc acccgtgggc tacaagccaa
      661 tcaatgttag tttcatattt cagtttcctg atgagagtac aattacaacc aatccaaccc
      721 aatgtgcata ctttaaataa ctcgtgcaaa tcaaaaagcg ggctaacaaa gcgtgctcct
      781 gacgctgggg attctgcggc aatctcatgc agttttctac gctgtatcat ttttctggtt
      841 ggacggcttc gccgtccccc gccccagcgc aggtaacgca tgccgttggg cggctgtagg
      901 cgggatattg tttcccgaaa taaggaatga aaaagaatgg caaaagattt aggcttttct
      961 tttgaaaaag catcaaacag taactataaa aaactcctca acatttctct ttgggctaat
     1021 aggttttctt gggtaatctt gttcttatac actattgatt tcttggctcg gacggcattt
     1081 cagatccaag aaatatttgg agaatggagc atcaaactta gcatatcctt tttggctaac
     1141 ctgttagcaa tacctgttgt tggaattata tattttttga ttttacaaac aatatcgggt
     1201 ggtatattaa tactcatgga taataataat cttgaattga ttcgtactct taatgtcaag
     1261 agaaataatg caatacagtt tattccgcac tgggcaaatg cgctctcttg ggtagtctta
     1321 tcattaaata ccatatattt cttggttaat ttagtgcttt actatccact tatatttgaa
     1381 aaagacagcg gatacgaact aggttatatg aatgaactag gtatgtgtac tcaattgttt
     1441 atcacactgg ttattggaat agtatatttt ttaattttgc aaacgatagc aagtagtatt
     1501 cttatattca aagagaaa
//
LOCUS       DAOQJE010000908         1517 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_79377_length_1517_cov_5.943228, whole genome shotgun sequence.
ACCESSION   DAOQJE010000908 DAOQJE010000000
VERSION     DAOQJE010000908.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1517)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1517)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1517
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_79377_length_1517_cov_5.943228"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..390
                     /locus_tag="PLT08_17830"
     CDS             <1..390
                     /locus_tag="PLT08_17830"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate hydratase"
                     /protein_id="HNE06392.1"
                     /translation="AGRDLAGVRKVVLDAVQKAQGQGCAPGVLGVSIGGDRGSSYIKS
                     KEVLFSEMGTRNEDPKLAELEERLTSEANEMGIGPMGFGGKTTVLDTKIIGLSRLPAS
                     YFVSVSYMCWAYRRRKMTVKGEEITYE"
     gene            409..1035
                     /locus_tag="PLT08_17835"
     CDS             409..1035
                     /locus_tag="PLT08_17835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FumA C-terminus/TtdB family hydratase beta
                     subunit"
                     /protein_id="HNE06393.1"
                     /translation="MKSITTPISDEAIRDLKVGDSVALSGMMVTGRDAAHKWMMETFI
                     KKTRAPQGDDLQVYEELKKLLHGSVIYHCGPVVSGLDTKEYKFLAAGPTTSIREEPYQ
                     ADVMKHFNVKGVIGKGGMGAKTLKGCQETPGVYLHAIGGAASFLAQTVVKVHGVFKME
                     FGVPEAMWVIEVKDFPVVVTMDSHGGSQHAVIDDASKKVLDGLLEKPY"
     gene            1117..>1517
                     /locus_tag="PLT08_17840"
     CDS             1117..>1517
                     /locus_tag="PLT08_17840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE06394.1"
                     /translation="MKKMRNFLRRLFAPSIFEDEEKTRTAQTLNVFSWVTFAVVFLLT
                     VSRILSGSWQSTSSKIFLPIILVVLLSVQVLTRYGYVRGAGRVAISMLWLSLSIQAYF
                     SDGLRDVTVLAFPILVLLSALLLGWQTGLLTG"
BASE COUNT          374 a          313 c          404 g          426 t
ORIGIN      
        1 gctgggcgtg atttagcagg cgtccgcaag gtggtgctcg acgcggtgca gaaggctcaa
       61 ggtcagggtt gtgcaccggg ggtcttgggc gtctccattg gtggtgatcg tggttcctcc
      121 tatatcaaat ctaaagaagt attgttcagt gaaatgggca cacgcaacga agacccgaaa
      181 cttgctgagc ttgaagaacg cctgacttct gaagccaacg agatgggaat tggtcctatg
      241 ggctttggcg gcaaaacgac ggtgctcgat accaagatta tcggcttgag ccgtttgcct
      301 gcatcctact ttgtgtctgt ttcttatatg tgttgggcat atcgccgccg caagatgacg
      361 gtgaagggcg aagaaattac ttacgaataa atcgtaagga gaataaccat gaaatcaatt
      421 actacaccta tcagtgatga agccattcgc gatctgaaag taggggactc agttgccctc
      481 tctggcatga tggtcactgg gcgcgatgcc gctcacaaat ggatgatgga aaccttcatc
      541 aagaagactc gtgcccctca aggtgatgat ctgcaagtgt atgaagaact gaagaagttg
      601 cttcatggct cggtcatcta ccactgcgga ccggttgtca gcggtttgga cacaaaagag
      661 tataaatttt tggcagccgg acccaccact tccatccgtg aagagccgta tcaagcagat
      721 gttatgaagc atttcaacgt caaaggtgtg atcggtaaag gcggcatggg tgccaaaacg
      781 ttgaaaggct gtcaggaaac tcccggcgtg tatcttcatg cgattggtgg cgcggcatcc
      841 ttcctcgcgc agacagtcgt caaagtccac ggtgtattca agatggagtt tggtgtgccc
      901 gaagcgatgt gggtcattga agtcaaagat ttccccgtgg tggtcaccat ggactcacat
      961 ggtggcagtc aacacgcggt gattgatgat gcctccaaaa aagttttgga tgggctttta
     1021 gagaaacctt actaaaacga taacttgagt gagttgcatt gcaactcact caagttattt
     1081 aaaatgatag ttttctttgg ggatacatgt tttataatga aaaaaatgcg taattttcta
     1141 agacgcttat ttgcgccatc catttttgaa gatgaagaaa agactcgcac agctcaaacg
     1201 ctaaatgtgt ttagttgggt tacgtttgct gtagttttct tgttgactgt atcgcgcatc
     1261 ttatcggggt catggcaaag cacatcctct aaaatttttc ttcccattat cttagttgtt
     1321 ttactgtcgg tgcaagtact gacccgttat gggtatgttc gtggagcagg tagggtggca
     1381 atctccatgt tgtggctatc attgagcatc caagcctatt tttcagatgg tttgcgcgat
     1441 gttacggtgt tggcattccc tatcttggtg ctattgtctg ctttgttatt gggttggcag
     1501 acaggcttgc ttacagg
//
LOCUS       DAOQJE010000909         1514 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_79756_length_1514_cov_1.986977, whole genome shotgun sequence.
ACCESSION   DAOQJE010000909 DAOQJE010000000
VERSION     DAOQJE010000909.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1514)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1514)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1514
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_79756_length_1514_cov_1.986977"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(88..1383)
                     /locus_tag="PLT08_17845"
     CDS             complement(88..1383)
                     /locus_tag="PLT08_17845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE06395.1"
                     /translation="MGFEVQEANQAILPQPRLPLMDDTGQLPLPQPVKVILSAQPDLS
                     KFTVEELKQVSTSQIGENEVEYKISGEITPELAEKIILAAPKKDREIVEKTIAVQQAQ
                     KPKTPSQRGEKFEVPQLCMFVDGQWEVATKDWFLDPRDWDLLKYPAELSEVDFSIKEE
                     ANQWLIDVQGKRVTRQFLGSETLFDLSDAPTNWTINQLSQELEKKVRQDFVRPQVLLE
                     FLRKTISFLNEKRNIPLSDLARARFILEKQLRDKIKFYYDDAMAKGYQASLFDLKAKV
                     KTNFEIEFSYPTDPFQYPANWYYKGGYIFNKNYYPLVGELESDGEEFDCAQAIDRNMA
                     IRCWVRNLSRRDNSFRLPTSTDYFYPDFVALLDDGRTLVIEYKGKPYETNDDSKEKIN
                     IGELWEKKSKGKGLFLFAVKRDAKGRDVYKQIEDKISGR"
BASE COUNT          396 a          320 c          281 g          517 t
ORIGIN      
        1 gaaaatgccg cagaatccca cacgtcaggt gcacgctttg ttgggttgct ttttgaatta
       61 caagactcac tggaattaaa catgagccta tcttccactt attttatctt caatttgctt
      121 ataaacatct ctgccttttg cgtctcgctt tacggcaaac aggaataagc ctttaccctt
      181 gcttttcttt tcccataatt caccaatatt gattttttct tttgaatcat cattggtttc
      241 atacggcttg cctttatatt ctataaccaa agtgcgccca tcatcaagca gggcgacaaa
      301 atcaggataa aaataatcgg tggatgttgg aagcctgaat gaattatctc gccttgatag
      361 atttcttacc caacatcgaa tagccatatt tctatcaatg gcttgggcac aatcaaattc
      421 ttctccatct gattctaatt ctccaacaag cgggtaataa ttcttgttga aaatataccc
      481 gcctttgtaa taccaatttg cagggtattg gaatgggtca gttgggtatg agaattcaat
      541 ttcaaagttt gttttcactt tggctttcaa gtcaaaaaga ctggcttgat aacctttcgc
      601 cattgcgtca tcataataaa acttgatttt gtctcgcagt tgtttttcaa gtatgaaacg
      661 agcgcgggca aggtcggaaa gaggaatatt ccttttttca ttcaaaaaag aaatcgtctt
      721 gcgtagaaat tcaagtagca cttgcggacg aacaaaatcc tgtctaactt ttttctctaa
      781 ttcttggctt aattgattta ttgtccagtt tgtaggtgcg tcacttaaat caaatagtgt
      841 ttcgcttccc aaaaattgcc ttgtaactcg cttaccttga acgtcaatca accattgatt
      901 cgcttcttct ttgattgaaa aatctacttc gcttaactcg gcggggtatt tcaataaatc
      961 ccagtctcgc gggtctaaaa accaatcttt tgtagcaact tcccattgac catcaacaaa
     1021 catacataat tgaggtactt caaatttttc gccacgttgg gatggtgttt ttggcttttg
     1081 cgcttgttga acggcaattg tcttttcaac aatttcgcgg tctttctttg gcgcggcaag
     1141 gatgattttt tctgccaatt cgggcgttat ctcgcctgaa atcttatatt caacttcatt
     1201 ttcgccaatt tgactggttg aaacctgttt caattcttcg acagtgaact tggataaatc
     1261 aggctgtgcg ctcaaaatta cttttacagg ttgcggtaaa ggtaattgcc ctgtatcgtc
     1321 cattaatggt aatcgtggtt gtggcagaat ggcttgattg gcttcctgca cttcaaaacc
     1381 catgctaatc atgcggtctt gtaactctgg aacggcattt ttccaactat cttctgaaac
     1441 atgagcataa gcgcggttta gttcttcttg tgttcgcttg gtggcgtagg gcattctcaa
     1501 aacacgccct aaaa
//
LOCUS       DAOQJE010000910         1512 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_79887_length_1512_cov_4.631434, whole genome shotgun sequence.
ACCESSION   DAOQJE010000910 DAOQJE010000000
VERSION     DAOQJE010000910.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1512)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1512)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1512
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_79887_length_1512_cov_4.631434"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(19..1119)
                     /locus_tag="PLT08_17850"
     CDS             complement(19..1119)
                     /locus_tag="PLT08_17850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE06396.1"
                     /translation="MDTAFHIIVFLLGAGLVIATIFSAISTFVLPRSARSQLNRLVFG
                     LTRRFIEIFMHFARTYEQRDAIMAYYAPLALMSLVPTWYMLIALGYSFMYWSLRAGDY
                     FFDLRLSGSSLFTLGFTASEGYFITILAFSEAMLGLIMVGLLIAYLPTMYSAFSRREQ
                     AVNMLEVRAGNPPNPYDMLSRFHRIHGLDKLAELWNNWEIWFADVEESHTTLPALVFF
                     RSPRSDNSWVTSAGAVLDSAAIALSSIDIPSEMSGALCIRAGFICFHRITDYFDIPHP
                     HDPKYPETPISVTRAEYDELIHKLEVAGVPIKPDREQAWKDFAGWRVNYDRTLLIMCA
                     LVMPPSNVPWSSDRAPKFKIPPLFIKKTKHQH"
     gene            complement(1186..1401)
                     /locus_tag="PLT08_17855"
     CDS             complement(1186..1401)
                     /locus_tag="PLT08_17855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE06397.1"
                     /translation="MVHGLEAEYFGRVKFSFLDADDPNTYNFQRELGFVYQPEFYLLD
                     ANGNVLKKLVGPVSEEEFRDLFDTNLQ"
BASE COUNT          460 a          348 c          373 g          331 t
ORIGIN      
        1 tctcggcact ctttttattt aatgttgatg ctttgttttc tttataaaca atggagggat
       61 tttgaatttc ggagcccggt cgctggacca tgggacgttg gagggtggca tcaccaatgc
      121 gcacataatc aacagcgtac ggtcatagtt gactcgccat ccggcgaaat ctttccaagc
      181 ttgttcgcgg tctggcttga tgggcacacc ggcaacttcc agtttatgaa tcaactcgtc
      241 atactcggcg cgagtgacac tgatgggcgt ttctgggtat ttggggtcgt gcgggtgcgg
      301 aatatcaaaa taatctgtga tgcggtgaaa acaaataaaa ccagcccgaa tgcacaaagc
      361 accagacatt tcggaaggaa tatcaattga tgaaagggca atggcagccg aatctaaaac
      421 ggctccagca gaagtgaccc aagaattatc ggaacgaggc gagcggaaaa agaccaaagc
      481 gggcaaagtg gtatgactct cttctacgtc ggcaaaccag atctcccagt tattccatag
      541 ttctgcgagt ttatccaaac cgtgaatgcg atgaaagcgg gagagcatat cgtaaggatt
      601 cggcgggttg cccgcgcgga cctccaacat attcaccgcc tgctcacggc gggaaaaagc
      661 agaatacatc gttggcaaat aagcgatcag caagcccacc atgatgagtc ctagcatggc
      721 ttcggagaag gcgaggatgg tgatgaaata tccctctgag gcggtaaatc ccaatgtaaa
      781 taaagaagag ccgctcaagc gcagatcaaa gaaataatcc ccagcccgta aagaccaata
      841 catgaaagaa tacccaagcg caatcaacat gtaccaagta ggcacaagcg acatcagtgc
      901 caaaggtgca taatacgcca tgatggcgtc tcgttgctca taggttcgag caaaatgcat
      961 gaagatttca ataaagcgac gggttagccc aaacaccagc cggttaagtt ggctgcgtgc
     1021 cgaacgtggc aaaacaaatg ttgaaatggc agaaaaaatg gtagcaatga ccaaccctgc
     1081 gccgagcaag aaaacgatga tgtgaaaggc tgtatccatg agccggaatt atattggagt
     1141 taaacaaaaa agagacagtc agttgactgt ctctcttaaa cctgcttact gcaaattcgt
     1201 atcgaacaaa tctctaaatt cttcttcaga aacgggaccg accaatttct tcaacacatt
     1261 tccatttgca tctagtaaat aaaattcggg ctgatacacg aacccgagtt cgcgctggaa
     1321 attataagtg ttgggatcgt cggcatccag aaaactaaac ttcaccctac caaaatattc
     1381 tgcttcaagc ccatgaacca tgggcgccat agcacggcag gtacttcagg taaagcggaa
     1441 gaattcgatc agctgcaaac cgccagaggc aaagctgacg gtggctgggt ctgtcgcttc
     1501 gaggttggga cc
//
LOCUS       DAOQJE010000911         1511 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_79988_length_1511_cov_5.459478, whole genome shotgun sequence.
ACCESSION   DAOQJE010000911 DAOQJE010000000
VERSION     DAOQJE010000911.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1511)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1511)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1511
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_79988_length_1511_cov_5.459478"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..439)
                     /locus_tag="PLT08_17860"
     CDS             complement(<1..439)
                     /locus_tag="PLT08_17860"
                     /EC_number="2.7.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="choline/ethanolamine kinase family protein"
                     /protein_id="HNE06398.1"
                     /translation="MNTLAIDKAIAKVPFLADSKNIKKIPLSGGITNLNFKIEADGRS
                     YVIRLAGEGTDQLGIKRDVEYAANKVAGELGIAPEVVYFIEPEGYIVTRFVTGKRIMP
                     DEIVKPDYLTRVAQKLRLFHRNAPKLKGEFNVFRRVEMLTKTAK"
     gene            complement(564..>1511)
                     /locus_tag="PLT08_17865"
     CDS             complement(564..>1511)
                     /locus_tag="PLT08_17865"
                     /inference="COORDINATES: protein motif:HMM:NF013720.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyltransferase family protein"
                     /protein_id="HNE06399.1"
                     /translation="VRGPGALKFLNYVCANQIDVPVGNLVYTQALNKRGGIECDFTVT
                     RLAEDRFFIVTGTAFGQHDMSWLSLQMPEDGSVTIEDVGSNYACIGMWGPKARTILEK
                     VTTDDVSNAGFPYMTAKNINVGDVPVRAARVTYVGELGWEFYCPMEYGLRLWDTLWEA
                     GQSEGMVAAGYKAIESLRLEKAYRYWSGEISPDYTPYEAGLGFAVKLDKENFLGKEAL
                     VKQKAEGVKTKLCTMTLADDRTIVMGKEPIRIDGKIVGWVASGGFGYSVAKSIAYAYL
                     PLEYSKPGTKLEVECFGEQVGAEVTANVLWDPKNERIKA"
BASE COUNT          351 a          399 c          368 g          393 t
ORIGIN      
        1 ctttggcggt tttggtgagc atttccacgc ggcggaagac gttgaactcg cctttcaact
       61 tgggagcatt gcggtggaac aagcgcagtt tttgcgcaac gcgcgtcaga taatctggtt
      121 tgacgatttc atctggcatg atgcgtttgc cagtgacaaa gcgggtcaca atataccctt
      181 cgggttcaat gaagtacacc acctcgggcg caatgcccaa ttcaccagct actttattag
      241 cggcatattc tacatcgcgt ttgatgccga gctggtcggt cccttcacct gcaaggcgaa
      301 tgacgtaaga acgaccatcc gcttcgatct taaaattcaa gttggtaatg ccaccactga
      361 gcggaatctt tttgatgttc ttagaatccg ccaaaaacgg caccttggca atggctttat
      421 cgatggcgag cgtattcatg tgactctagt tccttccttt ggttgaaaga attttttagc
      481 ggatgaagaa cccgcttctg tcctaactga aatctgcttc tgctttctcg agccttttct
      541 gtagttcgag atattctctc gaactacgcc ttaattcgtt cgttcttcgg gtcccacagc
      601 acattggctg tgacttccgc accgacctgt tcgccaaagc attcaacctc aagtttggtg
      661 cccggcttgg aatattccaa cggcaggtag gcatacgcaa tggatttcgc aactgagtag
      721 ccaaatccgc cagaggcgac ccagcccacg atcttgccat caatacgaat cggttccttg
      781 cccatcacaa tggtacggtc atcggcgaga gtcatcgtgc aaagtttggt tttgactcct
      841 tctgccttct gcttcaccaa agcttccttc ccgaggaagt tttctttatc aagttttaca
      901 gcaaagccaa gacccgcttc gtaaggagtg taatcgggcg aaatttcgcc gctccagtag
      961 cgataggctt tctcaaggcg tagcgactca atggctttgt aaccagctgc taccatgcct
     1021 tcagattgac cagcttccca gagcgtatcc cacaagcgca gaccgtattc catcgggcag
     1081 tagaattccc agccgagttc gcccacatag gtcacgcgtg cagcgcggac agggacatcg
     1141 cctacgttga tatttttcgc ggtcatgtac gggaagcccg cattcgagac atcatccgta
     1201 gtgactttct caaggatagt gcgagccttg ggtccccaca taccgataca ggcgtagttc
     1261 gagccgacgt cttcaatcgt cactgaacca tcttcgggca tctgtaatga aagccaagac
     1321 atatcgtgtt gaccgaaggc agtccctgta acaatgaaga agcggtcttc tgcgaggcgg
     1381 gtgacggtga aatcacattc gatgccgccg cgtttgttga gtgcttgcgt atagacaaga
     1441 ttgccaacag ggacatcaat ttgattggca cagacatagt tgaggaattt caacgcgccg
     1501 ggtccgcgga c
//
LOCUS       DAOQJE010000912         1508 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_80337_length_1508_cov_7.195458, whole genome shotgun sequence.
ACCESSION   DAOQJE010000912 DAOQJE010000000
VERSION     DAOQJE010000912.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1508)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1508)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1508
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_80337_length_1508_cov_7.195458"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..605
                     /locus_tag="PLT08_17870"
     CDS             <1..605
                     /locus_tag="PLT08_17870"
                     /inference="COORDINATES: protein motif:HMM:NF018652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE06400.1"
                     /translation="HGTPFGKWADKGILPLPDPDLAADMYEWTMEFLAENGYVQYEIS
                     NWAMGNEASHACKHNIQYWRSLPYLGLGAGAHGYAGGYRYSNVLRIKTYLDRLSNTQA
                     THLSYPLTPATVNQHKQTQRDDISDYMINNLRLVNAGIADSDFRSRFGAGLLDVFPKE
                     FEELIRNGLIEKKTSDGSEVFRLTHRGRLLGNQVFMRFVD"
     gene            complement(607..>1508)
                     /locus_tag="PLT08_17875"
     CDS             complement(607..>1508)
                     /locus_tag="PLT08_17875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE06401.1"
                     /translation="IPISAPVAKPYPDVTSKDTAPEQRAPYGDSFEINRLERPFTQDM
                     TYLPDMDIASFSISEDEQFYFVSIELVGKDPNNVAGIQYAVELDTNLDSFGDLLIVAS
                     PPFSEEWTAENIKIYTDTNRDTAGFSASSSDAPFSGNGYDTLIHDIVQGIGDDFDLAW
                     VRINADPQATVQFAFKKSFAGESFFYSVIADSGLKDVARLDYVDYFTEKDAGSPIRSN
                     LNYPLKALYAVDNTCYQVFGFKPSGYEPKICPEIVQPIQANPNQPIPVTPGVDYCTSI
                     GSPNPGNCPWGWSDAPYCVCTPG"
BASE COUNT          423 a          343 c          355 g          387 t
ORIGIN      
        1 agcatggcac gcccttcggc aaatgggcag acaaaggcat cctccccctc ccagacccag
       61 acctcgccgc agacatgtac gagtggacca tggagtttct agccgagaat gggtatgttc
      121 aatacgagat atcaaactgg gctatgggga atgaagcatc acatgcttgc aaacataata
      181 ttcaatattg gcgttcgctt ccatatctcg gtctaggcgc cggggcacat ggctatgcgg
      241 gcggctatcg ctattcgaac gtattacgca tcaaaactta tcttgatcgt ttaagcaata
      301 cccaagctac ccatctctcc tatcctctta cgccagctac agtcaaccag cataaacaaa
      361 cccaacgcga cgacatctct gattacatga tcaacaatct aagattagta aatgccggta
      421 tcgctgactc agatttcagg tccaggtttg gagccgggct tttagatgtc tttccaaaag
      481 agttcgagga gcttatccgc aatgggctca tcgaaaaaaa gacctccgat ggctcggagg
      541 tctttaggct tactcatcgc ggaaggcttt tggggaatca agtcttcatg cggtttgttg
      601 attaaattat ccaggcgtgc aaacacaata tggagcgtca gaccatcccc atgggcagtt
      661 tccagggttg ggactgccaa tcgaagtgca atagtctaca cccggtgtaa caggaattgg
      721 ttggttagga tttgcttgta tgggttgaac aatttcgggg catattttag gctcatagcc
      781 agaaggttta aacccaaaca cctgatagca agtattatcc actgcatata gagcttttag
      841 cggataatta agattgctgc gaattgggga gccggcatct ttttcagtaa agtaatccac
      901 ataatccaag cgggcaacat ctttcaaacc agagtcagcg atcaccgaat aaaagaagga
      961 ttctcctgca aaagatttct tgaaggcaaa ttggaccgtt gcctgcgggt ctgcattgat
     1021 gcgaacccaa gcgaggtcaa aatcgtctcc aatgccttgg acaatatcat ggatcaaggt
     1081 gtcatagccg tttcccgaaa acggagcatc ggaacttgaa gcagaaaagc cagccgtatc
     1141 ccgattggtg tctgtatata tttttatgtt ttctgcggtc cattcttcag aaaaaggcgg
     1201 gctggcaacg atgagtaaat cgccgaaact atccaaattg gtgtctagtt caactgcgta
     1261 ttgaatacca gccacattat ttgggtcttt cccaaccagc tctatagata caaaataaaa
     1321 ttgttcatcc tcactgatgc tgaaagaagc tatgtccatg tcaggaagat aggtcatatc
     1381 ctgtgtaaag ggacgctcaa gacggttaat ctcgaaagag tcaccataag gagcacgctg
     1441 ttcaggtgca gtgtcttttg atgtaacatc tggatagggc ttggctaccg gtgcagagat
     1501 tggaatgt
//
LOCUS       DAOQJE010000913         1504 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_80780_length_1504_cov_6.481021, whole genome shotgun sequence.
ACCESSION   DAOQJE010000913 DAOQJE010000000
VERSION     DAOQJE010000913.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1504)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1504)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1504
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_80780_length_1504_cov_6.481021"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..262)
                     /locus_tag="PLT08_17880"
     CDS             complement(<1..262)
                     /locus_tag="PLT08_17880"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HNE06402.1"
                     /translation="MKLSIIIPAYNEAKTIHLILNKVIETSLINEIQKEIIIVNDASK
                     DDTEKVIKEYIASHSDEDIRLFTLPYNQGKGAALHKGIELASG"
     gene            complement(322..744)
                     /locus_tag="PLT08_17885"
     CDS             complement(322..744)
                     /locus_tag="PLT08_17885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2200 domain-containing protein"
                     /protein_id="HNE06403.1"
                     /translation="MSVDSLMLSALPYWGIVDRNTGEVMRPKQQVFAMKFSDVYPLYI
                     QKAERKNRTKAEVDQIICWLTGYTQTTLQQQIKQEHDFETFFAQAPAIHPNTSLIKGV
                     VCGVRVEEIEDPLMQKIRFLDKLIDELAKGKAMEKILR"
     gene            complement(756..1127)
                     /locus_tag="PLT08_17890"
     CDS             complement(756..1127)
                     /locus_tag="PLT08_17890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HNE06404.1"
                     /translation="MTKYSMTPLNSGTRIRTDHSTFGSVITSVARGQLVVGDELWEAT
                     ADGTEVKKGDKWLHVTSVDGVNLAEAGWMAYIHKGVVICNNFTEIEEPNPNPTPVFPE
                     SFTLTDPSGAKAEYKFVRIIE"
     gene            complement(1124..>1504)
                     /locus_tag="PLT08_17895"
     CDS             complement(1124..>1504)
                     /locus_tag="PLT08_17895"
                     /inference="COORDINATES: protein motif:HMM:NF013357.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003796 - lysozyme activity [Evidence
                     IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA];
                     GO_process: GO:0016998 - cell wall macromolecule catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GH25 family lysozyme"
                     /protein_id="HNE06405.1"
                     /translation="GWRNWFDFMERIKTLLPEKKLGVYTGYYYFQEFAAGVNYFAQYP
                     LWIAWYNPTMPLIPPIWQDWTYWQFTDDGDGTLFGVESREIDLNYFNGSEAEFLARYP
                     APQTTEATLIAKFGNTLVEYRRVS"
BASE COUNT          373 a          318 c          322 g          491 t
ORIGIN      
        1 cccccgatgc cagttctatc cctttgtgta gagcagctcc ttttccttga ttatatggaa
       61 gcgtaaatag ccgaatatct tcgtctgaat gcgatgcgat gtattccttt attacctttt
      121 cagtatcatc tttagacgca tcattgacaa taataatttc tttttgaatt tcattaatca
      181 gcgatgtctc aattacttta ttgaggatta ggtgaatggt ctttgcctcg ttgtaggctg
      241 ggataatgat agataacttc atgaattgaa ttccttgatt ttttgttatt tgcttatgtt
      301 acatcatttt tataaatgat gctatcgtaa gatcttttcc atcgctttcc cctttgcgag
      361 ttcgtcgatt aatttatcta aaaagcggat tttttgcatt aatggatctt cgatttcttc
      421 cacacgcacg ccgcacacga cacctttgat aagagaagtg tttggatgaa tagctggcgc
      481 ttgagcaaaa aaggtttcaa aatcatgttc ttgcttaatt tgttgttgca gtgttgtttg
      541 ggtatagcca gttaaccagc aaataatctg atctacttcg gcttttgtgc ggttcttacg
      601 ctctgctttt tgaatataaa gcggatatac atctgaaaac ttcatggcaa aaacttgttg
      661 tttgggtctc ataacttctc cggtgtttcg atctacaatc ccccaatacg ggagcgctga
      721 cagcatcaag ctgtcaacgc tcattcctct aactattact caataattct tacaaactta
      781 tactctgctt tggcgccact tgggtctgta agtgtaaaag actcggggaa aacaggtgtg
      841 ggatttggat tcggttcttc tatttcagta aagttattac aaatgaccac acctttatgg
      901 atgtatgcca tccaacccgc ttcagcaagg ttgacaccgt ctactgatgt gacatgcagc
      961 catttatcgc cttttttcac ttcggttccg tcggcagtgg cttcccagag ttcatcgcca
     1021 actaccaatt gtccgcgtgc gacagaggta atcacagacc caaatgtgct gtggtcggta
     1081 cggatgcgag ttccgctgtt tagtggggtc atgctatatt tggtcatgag actctcctat
     1141 attcaacaag ggtattgcca aatttggcga tcagggtggc ttcggtagtt tgtggagcag
     1201 ggtatcgagc aaggaactcg gcttcactgc cgttaaagta atttagatcg atctcccggc
     1261 tctcaacgcc aaagagagtg ccatcgccgt catctgtaaa ctgccagtag gtccaatctt
     1321 gccagatggg tgggatgagc ggcatggtgg ggttgtacca tgcgatccat aatggatatt
     1381 gagcgaagta atttacacca gctgcaaatt cttgaaaata atagtagcct gtataaaccc
     1441 ctagcttctt ttctggtaat aaggttttga tgcgttccat gaagtcgaac cagttacgcc
     1501 agcc
//
LOCUS       DAOQJE010000914         1499 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_81319_length_1499_cov_3.592798, whole genome shotgun sequence.
ACCESSION   DAOQJE010000914 DAOQJE010000000
VERSION     DAOQJE010000914.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1499)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1499)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1499
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_81319_length_1499_cov_3.592798"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..574
                     /locus_tag="PLT08_17900"
     CDS             <1..574
                     /locus_tag="PLT08_17900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE06406.1"
                     /translation="ATLFLSTVLIFFYSPWRSLASFSFVIGMFIWTIQITYTKHEIGL
                     MQALKSGNLQQAKESVSIVSPSKLSRGEKLAFKAKEIVRQQVEINEDVLEAILAVQAS
                     STYYVYYIGLLAGNFIFVYTDFLGKPAGVERVGFSSIQDLTVEYGLLTDTLIMFIDGK
                     NPIPFKVNRGLRSHTEKFISMFKEKKSLLV"
     gene            775..1203
                     /locus_tag="PLT08_17905"
     CDS             775..1203
                     /locus_tag="PLT08_17905"
                     /inference="COORDINATES: protein motif:HMM:NF024861.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - endoribonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative toxin-antitoxin system toxin component,
                     PIN family"
                     /protein_id="HNE06407.1"
                     /translation="MKPPDIVLDTNVIIAALRSKIGASNKLLSLVGTHKFEIHDSVSL
                     ILEYEDVIQRQRTELGLSQDDVADFIDSLCSMAHHHQIYFLWRPTLSDVGDELVLELA
                     VTAGCEYIVTHNVSDFKNIGKFGIQAITPKEFLQVIREVK"
     gene            1203..1439
                     /locus_tag="PLT08_17910"
     CDS             1203..1439
                     /locus_tag="PLT08_17910"
                     /inference="COORDINATES: protein motif:HMM:NF017355.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin-antitoxin system HicB family antitoxin"
                     /protein_id="HNE06408.1"
                     /translation="MSALSLRLPKSLHEQLRELAQEEGISVNQFVMLAVAEKVAAIST
                     IEYLEKRAKRGSRERFLEILNKVPAVEPEEFDKL"
BASE COUNT          442 a          293 c          305 g          459 t
ORIGIN      
        1 ggctaccctt ttcttaagca ctgttcttat tttcttttat tctccttggc gttcattagc
       61 aagttttagt tttgttattg gaatgtttat ttggactata cagattacat atacaaaaca
      121 tgaaataggg ttaatgcagg cattaaaatc aggaaatctt caacaggcaa aggaaagcgt
      181 ctcaattgtg tcaccatcca aactgagtcg cggtgaaaaa ttggcattta aggcaaagga
      241 aattgtccgt caacaagtgg aaattaatga agatgtttta gaagcaattc ttgctgttca
      301 agcctctagt acttattatg tctactacat tggactctta gcgggaaatt ttatttttgt
      361 atacactgat tttttaggca agccagcagg agtagagcgg gttggttttt cttcaataca
      421 agacttaacc gtagagtatg gtttacttac ggatacattg attatgttca ttgacggcaa
      481 aaaccctatt ccattcaaag taaatcgtgg gttaaggtca catactgaaa aatttatttc
      541 tatgtttaaa gaaaaaaaga gtcttctggt ataaatgcgc ctaacaaagc gtgcacttga
      601 cgggtgggat tctgcggcat tttcgggcat tttcctacac tcgaacagaa tcctgctctc
      661 gaagttttgt ccacgcccgc ccacccgcaa gtaacgcaaa ccgttagacc cctccccaca
      721 aaattcatgt tttaggtggt tgatattaca taagatgtcg catataatac caatatgaag
      781 ccaccagata tagttcttga cacaaatgta atcattgccg cccttcgctc taagataggt
      841 gcatccaata aattattgtc ccttgtaggt acacacaagt ttgagattca cgattcggtt
      901 tcgctcatcc ttgaatatga agatgtaatc caacgccagc gaaccgaact tgggttatcc
      961 caagatgatg ttgcagattt tattgactca ttgtgctcga tggcacatca tcaccaaatc
     1021 tatttcttat ggcgtcctac cctttcagat gtaggtgatg aacttgtgct tgaattggcg
     1081 gtgaccgctg gatgtgaata catagtgacg cacaatgtaa gcgacttcaa aaatattggt
     1141 aagtttggta ttcaagccat taccccgaaa gagtttctac aagttatccg tgaggtgaaa
     1201 taatgagcgc attaagttta cgtttaccca aatctttaca tgaacagctc cgtgaattgg
     1261 cacaagaaga aggtatttct gtcaaccagt ttgtcatgct tgcagttgct gaaaaagttg
     1321 cagctatttc cacaattgag tatttagaaa aacgtgctaa acgcggtagt cgtgaaagat
     1381 ttttagaaat cctcaacaaa gtccctgctg ttgaacctga agagttcgat aaattgtaag
     1441 gggtctaaca aagcgtgcac ctgacgctgg ggattctgcg gcaatctcaa gcagttttc
//
LOCUS       DAOQJE010000915         1487 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_82659_length_1487_cov_3.761173, whole genome shotgun sequence.
ACCESSION   DAOQJE010000915 DAOQJE010000000
VERSION     DAOQJE010000915.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1487)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1487)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1487
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_82659_length_1487_cov_3.761173"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1487
                     /locus_tag="PLT08_17915"
     CDS             <1..>1487
                     /locus_tag="PLT08_17915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE06409.1"
                     /translation="SKQGQTFTPEQMAQIWNKKVNLIGGETYRLADMFPQVTPEQLTP
                     DALVDLMWNAQRNHRADKWWFYNNKKYTNTMQALEELAGELGSSLDEIATLDKSPTNP
                     YFELQKLQMKYQTAEEMFSWQRYLDQFTASPDVKLSQLAGDAKGRQLNQEYITRIVNT
                     TEAAKTAPRKANPLAYLKENGGINPEDFKNIFGESNASSVLPGLMKKNARAIDDVGIR
                     LWEGGFITREQADDYEYIRDFLRSFEKGFEKTDAQINAEEAAYWLEQAGQELVPYENI
                     TLKQAREALQNLKFVPSYDGGQISEARVAYEGMDNFLADVRRWGDKVETEWGKTIKST
                     FLENEDAMRSAQNAYEARMKVVRRKAAVVSVSTRNFALHDYNKTYMDHALTMMLGNSL
                     HYWSTRSYMRSIETLVDNPKIANTYMAYKEYMTREHSDMPEWYRYNIDVGALMGIDSN
                     NQYFINLESAINPMNGLTGVDFDDPQKRVDWMSRLVADVGKMGVG"
BASE COUNT          464 a          319 c          377 g          327 t
ORIGIN      
        1 ctcaaagcag ggtcagacct ttacgccaga acaaatggcg cagatatgga acaaaaaagt
       61 aaatttgatt ggcggagaaa cataccgcct tgcagatatg ttcccacagg taacacccga
      121 acaattaacc cctgatgcgc ttgtggattt gatgtggaac gcgcagagaa atcacagggc
      181 ggataagtgg tggttttaca ataacaagaa atacaccaac accatgcaag ccctcgaaga
      241 actggcgggg gaattaggct ctagtcttga tgaaattgca actctggaca aaagcccgac
      301 taatccatac tttgagttgc agaaattaca aatgaaatat cagaccgccg aggaaatgtt
      361 ctcatggcaa agataccttg accagtttac tgcatcgcct gatgtaaaac ttagccaatt
      421 ggcgggggac gcaaaagggc gtcaactgaa tcaggaatat attacgcgga ttgtaaacac
      481 cacagaggcc gctaagactg ctccgagaaa agcgaatcct ttagcctatc tcaaagagaa
      541 tggcggcatc aatcccgaag atttcaaaaa cattttcgga gaatcaaatg cctctagtgt
      601 gttgcctgga ttgatgaaga agaacgcccg cgctattgat gacgtaggta tcagactttg
      661 ggaaggtggg tttattacaa gagaacaggc ggacgattac gaatatatcc gcgattttct
      721 ccgaagtttt gaaaagggtt ttgaaaagac agacgcgcaa ataaacgcag aagaggccgc
      781 ttattggtta gagcaggcag ggcaggagct tgtgccgtat gaaaatatca ccctaaagca
      841 agcccgcgaa gccctgcaaa atctaaaatt tgtcccgtcc tacgatggcg gacagattag
      901 cgaagcccgc gtagcgtatg agggcatgga taactttctg gccgatgtca ggcggtgggg
      961 cgataaggtg gaaacagaat gggggaaaac gataaagtct acctttttgg agaatgagga
     1021 cgctatgcgg tcggctcaaa acgcctacga agcccgcatg aaagttgtca gacgaaaagc
     1081 ggcagtcgtt tccgtatcaa cgcggaattt cgccttacat gactacaata aaacgtacat
     1141 ggatcacgcc ctcacaatga tgctggggaa ctccctgcac tactggtcaa cccgttccta
     1201 tatgcggagt atcgaaacac tggtagataa tccaaaaatt gcaaatacct acatggctta
     1261 taaagagtac atgacccgtg aacactccga catgccagaa tggtatcgtt acaatataga
     1321 cgtaggcgca ttgatgggga ttgactcaaa taatcagtat tttatcaatc tcgaatccgc
     1381 tatcaaccct atgaacggat taacgggcgt ggactttgac gacccgcaaa aacgggtaga
     1441 ctggatgtcg cgccttgttg ctgatgttgg gaaaatggga gtggggg
//
LOCUS       DAOQJE010000916         1484 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_83005_length_1484_cov_5.055983, whole genome shotgun sequence.
ACCESSION   DAOQJE010000916 DAOQJE010000000
VERSION     DAOQJE010000916.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1484)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1484)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1484
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_83005_length_1484_cov_5.055983"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(130..408)
                     /locus_tag="PLT08_17920"
     CDS             complement(130..408)
                     /locus_tag="PLT08_17920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE06410.1"
                     /translation="MEKPPFSFVLIFLLFSFLFISNSYKLWFKTEAYYKDLYASLTNE
                     KIPLPFKGFFLKRLENKQSWLFWQKAFSLLGIVAVVGMDVLVVMAYLG"
     gene            complement(410..694)
                     /locus_tag="PLT08_17925"
     CDS             complement(410..694)
                     /locus_tag="PLT08_17925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE06411.1"
                     /translation="MIKGFLNLPWFVWAGLALVVALIYTFWVPQKIAPDISGIRLLIV
                     RWAHPLTWYLLALNFILRGISPEWGGIANMVAAAGGLMYMLFMLMTFVIK"
     gene            complement(694..1218)
                     /locus_tag="PLT08_17930"
     CDS             complement(694..1218)
                     /locus_tag="PLT08_17930"
                     /inference="COORDINATES: protein motif:HMM:NF024698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNE06412.1"
                     /translation="MPLHFTKLANPIPEEVYSAFTKWENDPALVHLTRPNQTQADIDR
                     KSEVDSFELDARISYQMTYLIYLDGQLVGEMSYQVDPQHLYKKETGTAWISITIGEES
                     ARGKGIGKLAMQYLEEEIHNAGYKRIELGVFEFNEPAQKLYQKMGYTEIGRIPNFTFW
                     QNKMWTDIRMEKYI"
     gene            complement(1237..>1484)
                     /locus_tag="PLT08_17935"
     CDS             complement(1237..>1484)
                     /locus_tag="PLT08_17935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE06413.1"
                     /translation="ELKIGVVVMESPNGFPLHETNIYLVAPSGDVVWKAEKPSANVLF
                     TKMKLNEDGSISTFTNSGQFCDINGENGKVISSSNFS"
BASE COUNT          407 a          337 c          310 g          430 t
ORIGIN      
        1 agttaagctc cttgatatga attcaattct ttaccacgac gggtcacaaa gtgtcacgaa
       61 ggttttcctt tgtgtgcctc accgcacttg cgtacggtgc aagtgttgtg accatttgtg
      121 gttaatttct tatcccaaat acgccatgac gaccaaaaca tccatgccga caactgccac
      181 aatgcccaat agactaaatg ctttctgcca gaacagccaa ctctgcttat tctccaaccg
      241 tttcaagaaa aaacccttga agggcagggg gattttttca tttgtcaggc ttgcatacaa
      301 gtctttatag tatgcctctg tcttaaacca gagcttatag ctgttcgaaa taaatagaaa
      361 gctgaataaa agaaagataa ggacgaaact aaaaggtggt ttttccatat tatttgatca
      421 caaaggtcat aagcataaac aacatataca tcaatccacc tgcagcggca accatattgg
      481 cgataccacc ccactctggc gaaataccgc gcaggatgaa attgagggcg agcaagtacc
      541 atgttagagg gtgtgcccag cggacgatta acaggcgaat cccgcttata tctggcgcga
      601 ttttttgagg aacccaaaag gtataaataa gcgcaacaac cagagcgagc ccagcccaaa
      661 caaaccacgg caggttaagg aaccctttga tcatcagatg tatttctcca tgcgaatgtc
      721 cgtccacatt ttgttttgcc agaacgtgaa gttgggaatt ctgccgattt cggtatatcc
      781 cattttttga tatagctttt gagcaggttc attgaattca aaaacgccta attcaatacg
      841 tttgtaacct gcgttatgaa tttcttcttc cagatattgc attgccagtt tgccaattcc
      901 cttacctcga gcagattctt cacctatggt gatgctgatc catgcggtgc cggtctcttt
      961 tttgtagaga tgctgcgggt ctacttggta actcatttcg cctaccagtt gaccatccaa
     1021 ataaattagg taggtcattt ggtaagaaat ccgtgcgtcc aattcaaacg aatctacttc
     1081 gctttttctg tcgatatctg cctgagtttg gttgggtcga gtaaggtgga caagtgcagg
     1141 atcattttcc catttggtaa aagcagaata gacctcttcc gggatagggt tcgctagttt
     1201 tgtgaaatgg aggggcatcc gcacttgttc ctgctattag ctgaaattgg aactactaat
     1261 taccttgccg ttctcgccgt taatgtcgca aaactgtcca ctgttcgtaa aagtggaaat
     1321 actgccatct tcatttagct tcattttggt gaaaagcaca ttggctgaag gtttttctgc
     1381 cttccaaaca acgtccccgc ttggagcaac caaatagatg ttggtctcgt gtaatgggaa
     1441 gccatttggg ctttccatga cgacaacacc tattttcaac tcaa
//
LOCUS       DAOQJE010000917         1478 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_83776_length_1478_cov_2.257203, whole genome shotgun sequence.
ACCESSION   DAOQJE010000917 DAOQJE010000000
VERSION     DAOQJE010000917.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1478)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1478)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1478
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_83776_length_1478_cov_2.257203"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            46..>1478
                     /locus_tag="PLT08_17940"
     CDS             46..>1478
                     /locus_tag="PLT08_17940"
                     /inference="COORDINATES: protein motif:HMM:NF012789.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HNE06414.1"
                     /translation="MAQIRRRDEISVGSIAQYELNSSVSKGANDVDYDKWQILLTSMQ
                     KDVVAKTLLVPIRLKGGPGTGKTLTAILRAANLLKQAKEKGESFRLGFFVFNRDLGRK
                     IYQQFVTLGLADFLKEDEPQQLVVTNLMEWCEKFLDLDNRGIEPIAPYRAERMDKQRE
                     ALYNLVIEEAKKSLMSQEYENLWKEFDVRSKNGLREIETEISQFIKAKDIRSLSAYLG
                     EHKPTQWVRQTDKEFRKFIWEVYQVYDATLKSLRMVDADDLVNDCFRQVSQTVWQKFD
                     KEKQGFDYLILDEAHDFFRHQIHLMAALAKDERNIMMCFDQGQMVYSRYPTLREMGYD
                     TDERFYIKRLEVNFRNSKQILASLQALIAAYPVYDYNSLWGQITSNPKSPESPKPNSQ
                     GYQSDSGMIRATAALVKKHIENGTDPREIAIICFDDDMFKNIESIVIKEVDVKIHHLT
                     GQGKHPPKNSIVLTTARQVKGEQFDVC"
BASE COUNT          506 a          271 c          301 g          400 t
ORIGIN      
        1 gtgcccactg taatggacta gaactgtatc agttacagtc gtcacttggc tcaaatacgg
       61 cggagagatg aaatatctgt tggttctatc gctcaatacg aacttaattc atctgtttcc
      121 aaaggcgcta acgatgttga ttatgataaa tggcaaatat tgcttacatc tatgcaaaaa
      181 gatgttgtag caaaaacgct ccttgtacca attagattaa agggaggacc tggcacaggt
      241 aaaactttaa ctgccatttt acgcgccgct aatttactta agcaggcaaa agaaaaaggc
      301 gaatctttta ggttaggttt ttttgtattc aaccgcgatt tagggagaaa aatatatcaa
      361 caattcgtaa ctcttgggct ggcagatttc ttgaaggaag atgagccaca gcaactagtt
      421 gtaaccaatt tgatggaatg gtgcgaaaaa ttcctagacc ttgataatcg cggtatagaa
      481 cccattgcgc cgtatcgtgc agaacgaatg gataaacaac gtgaagcact atataacttg
      541 gttattgaag aagccaaaaa atcgttaatg agtcaagaat atgaaaatct ttggaaagaa
      601 tttgatgttc gctctaaaaa cggtttacga gaaattgaaa cggaaatatc gcaatttatt
      661 aaagcaaaag acattcgttc attaagcgcg tatcttggcg aacacaaacc tacacaatgg
      721 gttaggcaaa ctgacaagga attcagaaaa tttatatggg aagtttatca agtgtacgac
      781 gcgactctta agtcgttacg catggtagac gcagatgatt tagtaaacga ctgcttccgt
      841 caagttagtc aaactgtctg gcaaaaattc gataaagaga aacaaggatt tgactattta
      901 attcttgatg aagcccatga tttttttagg catcagatac atctaatggc ggctcttgca
      961 aaagacgagc gcaatataat gatgtgtttc gatcaagggc aaatggttta ttcgcgttat
     1021 cctacattgc gcgaaatggg ctatgatacc gatgaaagat tctatataaa aagattagaa
     1081 gtaaatttcc gtaattcaaa acaaattctt gcatcactgc aagcattaat tgccgcatac
     1141 cccgtgtatg actacaactc actttggggg caaataacct caaacccaaa atctcccgaa
     1201 tctccaaagc ccaattcaca gggatatcaa agcgattctg gaatgattcg cgcaacagcc
     1261 gctttagtaa aaaagcatat cgagaacggg acagacccac gagaaattgc aataatctgt
     1321 tttgatgatg atatgttcaa gaacatcgaa tctatcgtta taaaagaagt ggacgttaag
     1381 attcatcacc tcactgggca aggtaaacac ccgccaaaaa actccattgt tttaactacg
     1441 gctaggcaag ttaaaggtga acaatttgat gtttgtat
//
LOCUS       DAOQJE010000918         1471 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_84514_length_1471_cov_3.823446, whole genome shotgun sequence.
ACCESSION   DAOQJE010000918 DAOQJE010000000
VERSION     DAOQJE010000918.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1471)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1471)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1471
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_84514_length_1471_cov_3.823446"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..484)
                     /locus_tag="PLT08_17945"
     CDS             complement(<1..484)
                     /locus_tag="PLT08_17945"
                     /inference="COORDINATES: protein motif:HMM:NF012312.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNE06415.1"
                     /translation="MSQQSVTASADQEEAQSLPRIIFASAAGTMIEWYDFYIFGSLAT
                     VIASKFYQTGTDIGDIIAWLATFAIGFIVRPFGAIVFGRVGDLIGRKYTFLVTMTLMG
                     LCTFAVGLLPTIEVLGSLAGVLLITLRILQGLALGGEYGGAATYVAEHSPHGRRGFYT
                     S"
     gene            complement(921..1358)
                     /locus_tag="PLT08_17950"
     CDS             complement(921..1358)
                     /locus_tag="PLT08_17950"
                     /inference="COORDINATES: protein motif:HMM:NF012791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HNE06416.1"
                     /translation="MYKKILVPLDGSTVAEGVLPHAKSLAYSEGAELILLTVAANPAM
                     DYLFSDPGIAETAVQEQVEKSKAYISTIENDLHSAGFKVSTLMRVGSVAEVILSVAEE
                     LQVDAIAMSTHGRTGAARWLLGSVAERVVHNSNVPVLLIRVKE"
BASE COUNT          414 a          378 c          324 g          355 t
ORIGIN      
        1 agcttgtata gaaaccgcgt cttccatgtg gtgaatgttc agccacatac gttgccgcgc
       61 cgccgtattc gccacccaat gccaaccctt gcaaaatacg aagcgtgatc aaaaggacac
      121 cggcgagtga gccgagaact tcaatcgtag gcaataagcc aactgcgaat gtacacagac
      181 ccatcaacgt catggtgaca aggaaggtat attttcgccc aataaggtcg ccaacacgtc
      241 caaacacaat cgcgccaaac gggcgcacaa taaaaccaat ggcaaaggtt gccagccagg
      301 caataatatc gccgatgtcg gtaccggtct ggtaaaactt actggcaatc acggttgcca
      361 gactgccaaa aatataaaaa tcgtaccatt cgatcatggt accggcagca gatgcaaaaa
      421 taatacgggg tagactttgc gcctcttctt ggtcagcgga ggctgttaca gactgttgag
      481 acataatgca gttctccttt gggaagataa atggatatgt tccgcgcatc gcggttgtaa
      541 caggctgggt gatcactagg cagaataagt catcagcgct cctttactcc gagaacatcc
      601 attaccccat ggacgagcag aagaagcgca tatcaaaccg cgtattgggg aaaagattct
      661 ttcgggcatt ggtacctcga acaaaaacct tgatccacct aaccactaaa gggtttgcat
      721 atttaaggaa ataacactaa gtttgccaaa ttcgttatgt tgatttcaac gccaaatgtc
      781 atggattttt ttgatgattg tcatatggca acataaaatt aaatttcaga caaaaaatgt
      841 ccgaaaaaaa ataacggctc tttcccgctc ttgccgggtg ctacactcaa aaaattagtt
      901 tccccgttag ctcaccccac ctactctttg acgcgaatca atagaactgg cacgtttgaa
      961 ttgtgcacaa ctcgttctgc cacactgccc agaagccaac gcgccgcacc cgttcgccca
     1021 tgcgtggaca tggcaatcgc atccacttgt aattcttctg ccacgctaag aatcacctcc
     1081 gctaccgagc cgacccgcat caaggttgag accttgaacc cggcagaatg aaggtcgttc
     1141 tcaatggttg aaatgtatgc tttgcttttc tcaacctgct cttgaaccgc ggtttctgca
     1201 atgccagggt cagaaaagag atagtccatg gctgggttgg cggcgacggt caatagaatt
     1261 aactctgcgc cttctgaata tgccagtgac tttgcgtggg ggagcactcc ctctgcaacg
     1321 gtcgagccat cgagtggaac cagaatcttt ttgtacataa cttcctccta tcggtttatt
     1381 gcccgaaacg tagacgtcgt atgagccgat tcgaaaactt tgcctgtttc atcagccctt
     1441 gaaccgttgt cacatcataa agaacccgct t
//
LOCUS       DAOQJE010000919         1470 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_84628_length_1470_cov_4.739223, whole genome shotgun sequence.
ACCESSION   DAOQJE010000919 DAOQJE010000000
VERSION     DAOQJE010000919.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1470)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1470)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1470
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_84628_length_1470_cov_4.739223"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..245
                     /locus_tag="PLT08_17955"
     CDS             <1..245
                     /locus_tag="PLT08_17955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE06417.1"
                     /translation="YSSTGKNNGDIRIKSDQIMKDGSERFEWTSKGGNYSGISFFEVR
                     GDDKRTWLMWTLVWNNTADQAVLDVIDSAIASYRIP"
     gene            324..1157
                     /gene="amrB"
                     /locus_tag="PLT08_17960"
     CDS             324..1157
                     /gene="amrB"
                     /locus_tag="PLT08_17960"
                     /inference="COORDINATES: protein motif:HMM:TIGR04336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system protein B"
                     /protein_id="HNE06418.1"
                     /translation="MMSSLDLRPSPIAGTWYPKDPEILANSIDAYLKEVQLLTLEGQV
                     LGVIAPHAGHRYSGAVAAHAFATLRGLTPDLVVVISPFHNFDPHPLLTTKHQAYATPL
                     GNIEVDQHTLNELRSHLQIPITPIAKDKEHSLEIELPFLQRVLKNDFKLLPIMVRAQE
                     ETVAKQLGEALAKTIQNRNAILVASTDLSHFYDQQTAQKLDQEMLNRFTTLNPTTIFE
                     AEQTGKGFACGHAAVATVQWVAKELGANNVQILKYATSGDVTGDFQSVVGYGAAAILR
                     T"
     gene            complement(1200..>1470)
                     /locus_tag="PLT08_17965"
     CDS             complement(1200..>1470)
                     /locus_tag="PLT08_17965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE06419.1"
                     /translation="KDGEGVTLYNYDEGEKVATPFAEQIEDVLRAKVESDPYLKSFTI
                     DFGTAADGGLEIWVNGEKYDGVANLPDEQLKQSLLEAVKDWNSRK"
BASE COUNT          402 a          393 c          290 g          385 t
ORIGIN      
        1 actacagcag caccggcaag aataacggcg acattcgcat caagagtgac cagatcatga
       61 aagacggttc tgaacgcttc gaatggactt ccaaaggcgg caattactct ggtatttcgt
      121 tctttgaagt tcgcggcgat gacaagcgca cctggttaat gtggactctc gtctggaaca
      181 acactgcaga tcaagctgtg ttggacgtga tcgacagcgc catcgcttcc taccgcattc
      241 cgtaatatag caagagagaa agtagtacta aatccccgcg aacattcgcg gggatttttt
      301 atttctccag gtgctacaat aaaatgatga gctctctcga cctgcgcccc tctcctattg
      361 ccggaacctg gtacccgaaa gaccctgaga tattagccaa cagcattgat gcgtatctaa
      421 aggaagtaca acttctaaca cttgaaggtc aagtactggg tgtgattgcg ccgcatgcag
      481 ggcaccgcta ttctggggca gttgccgcac atgccttcgc caccctgcgc ggacttaccc
      541 ccgaccttgt tgtagtcatc tccccttttc ataattttga cccacatccg cttctgacga
      601 ccaaacacca agcctatgcc actccgctcg gcaatattga agtagatcaa catacactta
      661 acgaactacg atctcactta caaattccca ttacacctat cgccaaagat aaagaacatt
      721 cacttgagat cgaactccct tttttgcaac gagttcttaa aaacgatttc aaacttttac
      781 ccatcatggt tcgtgcacaa gaagaaactg tcgccaaaca actaggggaa gccctggcaa
      841 agaccatcca aaaccgcaac gccattctag ttgcttccac agacctttct catttttatg
      901 accagcaaac ggctcaaaaa cttgaccaag aaatgctaaa tcgctttact acgcttaatc
      961 ccactacaat tttcgaagca gaacaaacag ggaaaggctt tgcttgcggt catgccgctg
     1021 ttgcaactgt tcaatgggta gctaaagaac ttggtgcgaa caatgttcaa atcttgaagt
     1081 atgcgacatc cggcgatgtc actggagatt ttcagtctgt tgttggctat ggagccgcag
     1141 caatcttgcg tacctaacaa aaagcgccag agaaatctct ggcgcttttt tggacttttc
     1201 tattttctac tgttccaatc ttttactgcc tcaagcaaag attgcttaag ctgttcatcg
     1261 ggcaagttgg caaccccatc atatttttca ccatttaccc aaatctccaa gccgccatca
     1321 gcagccgtgc caaaatcaat cgtgaacgat tttaagtacg ggtcgctttc cacttttgca
     1381 cgcagtacat cttcgatctg ttcggcaaat ggagtagcaa ccttttcacc ttcatcatag
     1441 ttgtatagcg ttacaccctc gccatctttc
//
LOCUS       DAOQJE010000920         1470 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_84631_length_1470_cov_4.292580, whole genome shotgun sequence.
ACCESSION   DAOQJE010000920 DAOQJE010000000
VERSION     DAOQJE010000920.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1470)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1470)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1470
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_84631_length_1470_cov_4.292580"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(358..>1470)
                     /locus_tag="PLT08_17970"
     CDS             complement(358..>1470)
                     /locus_tag="PLT08_17970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE06420.1"
                     /translation="GDPNFFAFGEYFDGSTPPFASFQILLQLDNENLKPFTKDALEFL
                     KNDKSAIELNWGFRAYSQFQDLLGLTMSNDSPVWNRHYCYYESLVYLRESITAWLDQN
                     VLAAMALIRPFLELSIFHLYWYLRCEHNEYTDFYLWLEGKKNKPPFKNQLDYVFENLP
                     TKDFVSSKQLEKVKEVLNNSYKSASTYNHTPKIDESIVSLGDGSGKTSFYLFFFYIAS
                     LNLVVRQLTYLYILAYPMSLFPVDRHKKWGYGGPVGLFFDFSNFAIIKEYVGMDNIQK
                     MKADFPKLQEVKDLSSWYEGQPDLSESEIENTWQEFISDKPNSDINIKEHRMAFFRAH
                     FRGLGWFVNYFHEPPKGDDEISDEMLEKVMRRINNW"
BASE COUNT          441 a          289 c          268 g          472 t
ORIGIN      
        1 ctctgttgaa ctcaataaat atactaatcc gtgttgttgc tgtttcaaaa tcataattga
       61 ggtcactttt cttcttcaac acaaaatgat actcgccctc taaaaaacca aaatgctctt
      121 taatcaaagt atcaaattct tgtgtaggta tatttgacat tatttctcct aatgcttttt
      181 cgctgaagta attctgcctg tgatgaaacg cctaacggct tgcgttacct gcgctggggc
      241 agggacggcg aagccgtcca gccagaaaaa tgataaggcg tgtgaaactg cttgggatgt
      301 gcgcagaatc cccagcgtca ggtgcacgct ttgttggctt gctttttgtt cgcagactca
      361 ccaattgttt attcgtctca ttactttttc tagcatttca tctgaaatct catcatcgcc
      421 tttgggtggt tcatgaaagt aatttacgaa ccaaccaagc ccacgaaaat gcgctcgaaa
      481 aaatgccatt cgatgctctt tgatatttat atcagaattt ggtttatcgc tgataaattc
      541 ttgccaagta ttttcaattt ctgactcgct taaatcgggc tgtccttcat accatgaaga
      601 taagtccttc acttcctgta actttggaaa atcggctttc attttttgta tattatccat
      661 gcccacatat tcttttatta tcgcaaaatt tgaaaagtca aaaaataacc caacaggacc
      721 tccgtagccc cattttttat ggcggtctac gggaaataat gacattgggt aagcaagaat
      781 ataaagatat gttaactgac gaacaaccaa atttaggctc gctatgtaaa agaagaaaag
      841 ataaaacgaa gttttccctg atccatcgcc caaacttaca atggattcat caattttggg
      901 tgtgtggtta taggtgcttg cgcttttata cgaattgttt aatacttctt tgactttttc
      961 taattgtttt gatgaaacaa agtctttggt tggtagattt tcaaaaacat aatccaattg
     1021 attcttgaac ggcggcttgt tctttttgcc ttccaaccaa agataaaaat ctgtgtactc
     1081 gttatgttca cagcgaagat accagtaaag atgaaatata gacaattcaa gaaatggtct
     1141 tataagtgcc attgccgcaa ggacgttttg gtctagccaa gcagtaatac tttctcgaag
     1201 ataaactaaa ctctcgtagt agcaataatg tctgttccat actggactgt cattgctcat
     1261 tgttaagcca agcaaatcct gaaattgtga ataagcccga aagccccaat ttaattcgat
     1321 tgcactttta tcgtttttta gaaactctaa agcgtcttta gtaaaaggtt tgaggttttc
     1381 attatctaac tgtaacaaaa tctgaaaaga agcaaaagga ggtgtggagc catcgaaata
     1441 ttccccgaat gcaaagaaat tggggtcacc
//
LOCUS       DAOQJE010000921         1468 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_84873_length_1468_cov_4.729653, whole genome shotgun sequence.
ACCESSION   DAOQJE010000921 DAOQJE010000000
VERSION     DAOQJE010000921.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1468)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1468)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1468
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_84873_length_1468_cov_4.729653"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            21..>1468
                     /locus_tag="PLT08_17975"
     CDS             21..>1468
                     /locus_tag="PLT08_17975"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HNE06421.1"
                     /translation="MTSLKLFKKELPSWLPLVLAGVGAILYLIQAIIYAHTTVSSLDE
                     GSYLIKGMFYLNGVYKPFEPYGPLTNKAPFAFLIPGFAEYLFGAGLRTGRYFSIFLGL
                     LTILGVWVTARRWTNNWLAAFTIWVFALSPMIIKLHARAVSEVIIACILAWICVLILD
                     VERPVWQIVLASALSAVAVFTRQNMAPILPFLIIYTFWQHGKQKGMWAFATGSFFFLI
                     FHIFYWPRVMTIWAPWLPESLTPFLNSFRIPTDSVPIWDPNIDFWNRLNSFFQGLRYH
                     FIPLVGGLFGLILLPPRTNWKSVPAMRAAVFLAVSYFVLAAMHGWAAIASQYESYSCV
                     FCFSNYLGFFDPLGILFFVISFAYAWNQNPNRVVQIFSVVVVLVFATGIGYSLFEQVG
                     NGLLNLPIVPRIRSGGIGLVAIVDALTQGLDMPLVQVKRWISSMLGLFVGFGLLLTSF
                     FTWKRAQKPNFTLVLANSFLITGFVLSPVLHLG"
BASE COUNT          286 a          327 c          325 g          530 t
ORIGIN      
        1 gactgtcacc tgctttaatt atgacctctt tgaaattatt caaaaaagaa ttgccttcct
       61 ggctgccgct cgttctggca ggtgtggggg cgattctgta tttaattcag gcgatcattt
      121 atgcccatac aacggtcagt agcttggatg agggttccta ccttatcaaa ggcatgttct
      181 atttgaatgg agtttacaaa ccattcgagc cgtatggtcc gctgaccaat aaagcgcctt
      241 ttgcatttct aattccggga tttgctgaat atctttttgg tgcagggtta cgaacggggc
      301 ggtatttctc catctttctt gggttgctaa ccattcttgg tgtatgggtc actgctcgac
      361 gttggacaaa taattggctg gcagcattta ctatttgggt atttgctctc agtcccatga
      421 ttattaagtt gcatgcccgt gctgtatcag aagtcattat tgcatgcatt cttgcatgga
      481 tctgtgtgct catacttgat gtagaacgtc ccgtgtggca gattgttctt gcatctgcat
      541 tatctgcggt tgctgttttt acacgccaga acatggcgcc tattctgccg ttcttgatta
      601 tttacacctt ttggcagcat ggaaaacaaa aggggatgtg ggcttttgcc acaggttcgt
      661 tcttttttct aatctttcat attttctact ggccccgtgt tatgacgatt tgggcaccgt
      721 ggctcccaga gagtttaact ccttttctta attcatttcg tattccaacc gattcggttc
      781 caatctggga tccgaatatc gatttctgga ataggctcaa ttctttcttt caaggtctcc
      841 gatatcattt tattcccctt gttggcggac tcttcggttt gattcttctc ccgcctcgta
      901 caaactggaa gtctgttcct gccatgcgag cggctgtttt cctcgctgtg agttattttg
      961 tgttagccgc tatgcacggc tgggcagcga ttgcgagcca atatgaaagc tattcttgcg
     1021 ttttttgttt ttcgaattat cttggctttt tcgacccgct tgggattctt ttttttgtaa
     1081 tttcatttgc ctatgcttgg aaccaaaacc cgaaccgtgt ggtacagata ttctctgtcg
     1141 ttgtagttct tgtttttgcc acaggcattg gatactcgct ctttgaacaa gttggtaatg
     1201 gattgctcaa tttgcccatc gtgcctcgta ttcgttcggg cggcattgga cttgttgcca
     1261 tcgtcgatgc actgactcaa gggctcgata tgcctctagt tcaagtcaag cgttggatca
     1321 gttcaatgct tggcttattt gtcggcttcg gactcttgct tacatcattt ttcacttgga
     1381 agcgcgctca aaagccgaac tttacattag ttcttgctaa ctcatttctt atcactggat
     1441 ttgtcctatc tcctgttttg catcttgg
//
LOCUS       DAOQJE010000922         1467 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_85012_length_1467_cov_6.511331, whole genome shotgun sequence.
ACCESSION   DAOQJE010000922 DAOQJE010000000
VERSION     DAOQJE010000922.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1467)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1467)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1467
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_85012_length_1467_cov_6.511331"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1145)
                     /locus_tag="PLT08_17980"
     CDS             complement(<1..1145)
                     /locus_tag="PLT08_17980"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNE06422.1"
                     /translation="MKTFFLATYLALKEVFRNRGRFLLVALVIALITMLVLFIAALGE
                     GLGNGNRQYIANLDADLIVFLEKSDYIISASRLEVNTARSIRRVEGVADAGAIYTSST
                     EIVSLEEPLKVSMLGVEPGRPGMPTILEGREFRTGEAREAVIDRNVALRSDIKIGDTI
                     RIRSTQGTDDEFYELKVVGLVDGQSYTFQPTIFVPPATWEKIRPQSESDMVSDTPYPN
                     VVAVKLQDPAQIDAMSARLMERIPNIEVADIQTTISNIPGYSAQQGTVQTQAVFTLLI
                     GTLVIGGFFQIQILQKVPQIGVLKAIGSANGMVGLSAVIQIIVVTAMGVGIGSGLTYF
                     FSLGFPPTIPIVFNGVNSLIAVILLLLIGPIGGMVSIIYAVRIEPLKA"
     gene            complement(1159..>1467)
                     /locus_tag="PLT08_17985"
     CDS             complement(1159..>1467)
                     /locus_tag="PLT08_17985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE06423.1"
                     /translation="DPDRTVYKAYGLYRGTLWQTILSPNIWKSNRRLARQKGIKPGVP
                     SPGQDVYQMSGTFVIGMDGKIRLPYYYEDIADHPPVDLLLHGIMGVDWKKPFDSNPLV
                     "
BASE COUNT          421 a          361 c          339 g          346 t
ORIGIN      
        1 gccttgagcg gctcaatacg aacagcataa atgatagaga ccattccacc gatcggtccg
       61 atcaacagaa gcaagatcac agcgatgagt gaattaacac cattgaacac aatcggaatg
      121 gttggaggga acccaagcga gaagaagtag gttaagccgc tgccaatgcc cacccccatg
      181 gcggtcacaa caatgatttg aatcactgcc gaaagcccta ccattccatt agcagaccca
      241 attgccttca acacaccgat ctgcggaacc ttttgaagaa tctgaatctg gaagaagccg
      301 ccgataacaa gagtgccgat caataaagta aagaccgcct gcgtttgaac tgtcccttgc
      361 tgggcagaat atccaggaat attactgatg gtcgtttgaa tatctgccac ttcaatattg
      421 ggtatacgtt ccatcaaacg ggcagacatt gcatcaatct gagcagggtc ttgcaacttc
      481 acagctacca cgttgggata cggtgtatca ctgaccatgt ccgattcaga ttgagggcga
      541 atcttttccc atgtggctgg cgggacaaaa atagtgggct gaaaggtata tgattgacca
      601 tcaactaagc caacgacctt aagttcataa aattcatcgt cggtgccttg ggtggagcgg
      661 attcgaatcg tatcgcctat cttaatatct gaccgcaaag ctacgttgcg gtcaatcacg
      721 gcttcacgcg cttcaccggt acggaattca cgcccttcca aaatagtagg catgccgggg
      781 cgacctggtt ctacacccag cattgatacc ttcaagggtt cttctaacga cacgatttct
      841 gtgctggatg tatagatcgc gccagcatct gccacacctt ccacacgtcg aatggaacga
      901 gcggtgttca cttccaagcg gctggcagag ataatgtaat ctgatttctc caaaaagacg
      961 atcaggtctg catctaagtt ggcaatatat tggcggttac cattgcccaa cccctcccct
     1021 aatgcagcaa tgaagaggac taacatcgtg atcaacgcga tgacgagcgc cacaagcaaa
     1081 aaacgtccgc gattgcggaa cacttccttg agcgcaaggt aagttgcaag aaaaaaagtt
     1141 ttcattattt cgtacttcct agaccaaagg gtttgagtcg aacggtttct tccaatccac
     1201 acccatgatg ccatgtaaaa gcaaatctac aggtggatga tcggcaatat cttcgtagta
     1261 ataaggcaaa cgaattttcc catccatacc aataacaaag gtgccagaca tttgataaac
     1321 atcctgtccg ggggatggta caccaggttt gatgcctttc tgtcgcgcca accttctatt
     1381 ggacttccaa atattggggg aaaggatggt ttgccagaga gtgccacggt aaagaccata
     1441 ggctttgtaa acggtacggt ctgggtc
//
LOCUS       DAOQJE010000923         1459 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_85948_length_1459_cov_5.766382, whole genome shotgun sequence.
ACCESSION   DAOQJE010000923 DAOQJE010000000
VERSION     DAOQJE010000923.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1459)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1459)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1459
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_85948_length_1459_cov_5.766382"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..887
                     /locus_tag="PLT08_17990"
     CDS             <1..887
                     /locus_tag="PLT08_17990"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014625.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019213 - deacetylase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HNE06424.1"
                     /translation="PAKSSPSITSLTVTPVAVSFLGAINMTQLKLLAIFAHPDDEAMG
                     MGGTLAKYAAEGVETHLVCASRGEVGWFGPEEQNPGPERLGQLRTEELQNAVNTLGMK
                     SLSFLNYLDGQVDQAEPTEAIAKIVTHIRRIQPQVVVTFPHDGNYGHPDHIAIGQFTN
                     AAIVCAADSSYKDPENLPAYRVSKLYYMVDGESFINLIAPFMGDMTFPVDDQLRGENA
                     WKEWMITTKIDMAEHCHTAWKAIMCHKSQLATLGALAEMHEDAAVSVLAMQGTFFRAF
                     SLVNGGRKLENDFFEGLR"
     gene            complement(928..>1459)
                     /locus_tag="PLT08_17995"
     CDS             complement(928..>1459)
                     /locus_tag="PLT08_17995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE06425.1"
                     /translation="KSAGDEKTRLEGIREKLLGFVADMDKQMEARLKQAQDFVEALLA
                     APDIEQAVTANLDKFTQDSVEVAQQMLRQATEKNEYERMGKLQKMIQVLQAASAPPEI
                     SIIEQLIQLPDAATIEQALTENPEIVTQQLLDTLTGLATQMESQADNPEAKAMGEKLS
                     EIYKIALKLSMKKNIG"
BASE COUNT          346 a          364 c          359 g          390 t
ORIGIN      
        1 accctgcaaa aagcagccct tcgataacct cattgacagt cactccggtg gctgtcagtt
       61 ttttaggagc aatcaatatg acacaactta aactgctggc gatctttgcg caccccgatg
      121 atgaagctat gggcatgggc gggacgttgg cgaagtatgc cgccgaaggc gtggagactc
      181 atctcgtctg cgcttcacgc ggagaggtgg gctggttcgg ccccgaagaa cagaatccag
      241 gtcccgagcg acttgggcaa ctgcgcaccg aagagcttca gaacgccgtc aacacattag
      301 gcatgaaaag cctttctttt ttaaattacc tcgatggtca agtggaccaa gccgagccaa
      361 ctgaagcaat tgcgaagatc gttacacata ttcgtcgcat ccaaccgcaa gtagtggtga
      421 ctttcccgca tgatggtaat tacggtcatc ccgatcacat cgctatcgga cagttcacca
      481 atgctgccat cgtctgcgcc gcggactcaa gctacaaaga ccctgagaat cttcctgctt
      541 accgtgtctc taaactttat tacatggtag acggtgaaag tttcattaac ttgatcgcac
      601 cctttatggg cgatatgacc ttcccggttg atgaccaact ccgtggcgaa aatgcttgga
      661 aagaatggat gatcaccact aagatcgata tggcagaaca ctgtcacacc gcatggaagg
      721 cgatcatgtg ccacaaaagc caattggcta ccttaggcgc tctcgccgaa atgcacgaag
      781 atgcagccgt gtcagtcctt gccatgcaag gaactttctt ccgcgccttt agtttggtga
      841 atgggggcag aaagttagag aatgatttct ttgaagggtt gagataataa aaaagaaggt
      901 cacgctacta caacgtgacc ttctttttta tcctatattt ttcttcatcg agagcttgag
      961 cgcaatcttg taaatttcac tcagcttctc tcccatggct tttgcttcgg ggttatcggc
     1021 ttgtgattcc atttgcgtgg cgagacccgt cagagtatca agcaattgtt gcgtaacgat
     1081 ctctgggttt tccgtcagcg cttgttcaat ggtggcggca tcgggcaact ggatcaactg
     1141 ctcaatgatg gaaatctcag gcggggcgct ggcagcttgt agcacctgaa tcatcttttg
     1201 caatttgccc atgcgttcgt attcattttt ctcagtagct tgtctgagca tttgctgggc
     1261 aacttctact gaatcttgcg tgaacttatc gaggttggca gtgacggctt gttcaatatc
     1321 tggggcagcc aacagagcct caacaaagtc ttgggcttgt ttgaggcgcg cttccatttg
     1381 cttgtccata tcggctacga acccaagcaa cttctcacgg attccttcga ggcgggtctt
     1441 ttcgtcacct gccgatttg
//
LOCUS       DAOQJE010000924         1456 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_86333_length_1456_cov_5.386867, whole genome shotgun sequence.
ACCESSION   DAOQJE010000924 DAOQJE010000000
VERSION     DAOQJE010000924.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1456)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1456)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1456
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_86333_length_1456_cov_5.386867"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..402)
                     /locus_tag="PLT08_18000"
     CDS             complement(<1..402)
                     /locus_tag="PLT08_18000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyceraldehyde 3-phosphate dehydrogenase
                     N-terminal domain-containing protein"
                     /protein_id="HNE06426.1"
                     /translation="MAIKVGINGFGRIGRQVLKAIRDYYPKELEVVAFNDIGDTKTMA
                     HLLKYDSTYGRFNGTVEVAEDGLLIDGKKVKVYKETDPAAIPWKDLGIDIVIESTGLF
                     TVKEDGVNKKGKTVKGAVNHITSGGAKKVIIS"
     gene            complement(566..>1456)
                     /locus_tag="PLT08_18005"
     CDS             complement(566..>1456)
                     /locus_tag="PLT08_18005"
                     /inference="COORDINATES: protein motif:HMM:TIGR01826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YvcK family protein"
                     /protein_id="HNE06427.1"
                     /translation="GLKTHTRNLTAVVTVADDGGSSGKLRETFGILPPGDIRNCLAAL
                     SNDEAMLTQLFQYRFSGAPGLEGHSFGNLFITALADITGSFEAAVVESGRVLSVNGRV
                     LPSTLHNVQLVASLELPHTLNEVRVEGESKIPEMAGRVRRVWLEPDDAPAFPPVIKEL
                     LTADIVVVGPGSLYTSLLPNLLVHDLLDSLHASRAVKIYVCNIATQSGETDAFTCYDH
                     VRALEGHVGENLFDIILCNDNYKGDLGPSSVWVRADEKTLSDERTYTADLIDDAHPWR
                     HDSTKLAQAILDIFSQKFQP"
BASE COUNT          408 a          358 c          286 g          404 t
ORIGIN      
        1 agagatgatt actttcttgg cgccgccaga agtaatatga ttgacagcac ccttaacggt
       61 cttgcccttc ttgttcacac catcttcttt gacagtgaac aaaccagtgg actcgatcac
      121 gatgtcgata cccaaatctt tccaaggaat ggcagccggg tcagtttcct tatagacctt
      181 gaccttctta ccatcgatca ataaaccatc ttctgcaact tcaacagtgc cattgaaacg
      241 accataggtt gagtcgtatt tgagaagatg agccattgtc ttggtgtcgc caatgtcatt
      301 aaatgcgaca acttcgagct cctttgggta atagtcacga atcgccttca aaacctggcg
      361 accgatacga ccaaatccgt taatacctac tttaattgcc atggggaatt cctccaatta
      421 aatttatctc agggaacacg cttgtgttgc ctctggtatg gtcattcata caacttgaat
      481 tttacttctt ttgagcaaat ttgtgaattt tcgtacgtct gggtttttgg tgataaatgt
      541 aatcgattac ttctataatt ttagcctatg gttgaaattt ctgactaaaa atgtccaaaa
      601 tcgcctgcgc caatttggtt gagtcatgcc gccaaggatg agcatcatct atcaagtcgg
      661 cagtatatgt tctctcatct gaaagtgttt tttcatctgc tcgcacccaa actgatgaag
      721 gtcccaaatc gcctttgtaa ttatcattgc ataaaataat gtcaaataag ttttcaccca
      781 catgcccttc caaagcacgg acatggtcat agcaagtaaa ggcgtctgtc tcaccagatt
      841 gtgtagcaat attacacaca tagatcttaa cggcacggct ggcatgcaaa ctatctaaca
      901 gatcatgaac taagagattg gggagcaaac ttgtatataa actccccgga cctacaacca
      961 caatatccgc tgtcagtagt tctttgatca caggaggaaa tgctggagca tcatccggct
     1021 caagccagac gcggcggacc ctcccagcca tttccggaat cttactctcc ccttctactc
     1081 gaacttcatt cagagtgtgt ggcaattcga ggcttgcaac caattgaaca ttatgcaatg
     1141 tagatggtaa tacgcgccca ttgacggaaa gaacgcgtcc cgactcgacc acagctgcct
     1201 caaaacttcc tgtaatatca gccaacgctg tgataaatag gttcccaaaa gagtgtccct
     1261 ccagccccgg tgcaccgcta aagcgatatt gaaaaagctg tgtcaacatt gcttcatcat
     1321 tagacaaggc tgcaagacag ttacgtatat caccaggtgg aagaatccca aaggtctcac
     1381 gcaatttacc agacgagcca ccgtcatctg caacggttac cactgcagtc aagtttcgtg
     1441 tgtgtgtttt taatcc
//
LOCUS       DAOQJE010000925         1456 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_86365_length_1456_cov_3.033547, whole genome shotgun sequence.
ACCESSION   DAOQJE010000925 DAOQJE010000000
VERSION     DAOQJE010000925.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1456)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1456)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1456
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_86365_length_1456_cov_3.033547"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1456)
                     /locus_tag="PLT08_18010"
     CDS             complement(<1..>1456)
                     /locus_tag="PLT08_18010"
                     /inference="COORDINATES: protein motif:HMM:NF019352.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="multicopper oxidase domain-containing protein"
                     /protein_id="HNE06428.1"
                     /translation="LAACAGADFQQVDKEYVLTTDLRNGNLVFLGVSDEINGQENPTL
                     SAKAGERITITLINGGEGEHSITLPELNASSEIVKEKGEETSITITVPNVHGEIGYYD
                     GVGNHADLGMRGKLVVTETGAAMPAMNTSTTSNGDPAVLAAFQKGACGSCHTISGIPS
                     AVGVVGPKLDDISAMSEEMIKDTSYSGHATNAEEYIRESITDPNLFLAPECPTGACAP
                     NVMPATLTQTLNEEEINAIVTYLTGLPDGAYKESTTTTEAVLAPQEPDNSGVDIIRDP
                     TDIPAPVGNREPQIVRIDLEATEVVGQLADGTTYTYWTFNNQVPGPFFRVRVGDTIEV
                     HMKNSLSSTMNHSVDFHAVTGPGGGAVMTQTEPGKETVFTAKAINPGLFVYHCATPMV
                     AQHITNGMYGLILVEPEGGLPPVDREFYVMQGEIYTAGAFGDQGHQMSDTTKLLGEEP
                     EYFVFNGAVNALKDKKPLKANVGESIRIFFGVGGP"
BASE COUNT          299 a          341 c          427 g          389 t
ORIGIN      
        1 ggtccgccga caccgaagaa gatgcgaatg ctttcgccga cattggcttt gagcggcttc
       61 ttgtctttga gggcgttgac ggcaccattg aaaacgaagt attcaggttc ttcaccgagc
      121 agtttggtgg tatcgctcat ttgatgacct tgatcgccga atgcgccagc ggtgtagatt
      181 tcgccttgca ttacgtagaa ttcgcggtct actggaggca gcccaccttc aggttcaaca
      241 aggatgagac catacatccc attggtgatg tgttgggcga ccatgggggt agcacagtgg
      301 tagacgaata aaccggggtt aatcgctttt gcagtaaaaa ctgtttcttt gccgggttcg
      361 gtttgggtca tcactgcgcc gccgccaggt ccggtcacgg catggaaatc gaccgagtga
      421 ttcatggtgc ttgaaaggct gttcttcata tgaacttcga tcgtatcgcc gacacgcacg
      481 cggaagaacg gtccggggac ttggttgttg aaggtccaat aggtgtaggt ggtgccgtct
      541 gcgagttgac cgacaacctc agtcgcttcg aggtcgatgc gtacaatttg cggttcacga
      601 ttgccaacgg gggcggggat atcggtaggg tcacggatga tatcgacacc gctgttatca
      661 ggttcttgtg gagccaatac tgcttcagta gttgtcgtgg attctttgta cgcgccatct
      721 ggaagaccag ttagatatgt gacgatggcg ttgatttctt cttcgttcag ggtctgagtt
      781 agagtggcgg gcatcacgtt tggagcacaa gcaccagtgg ggcactcagg cgcgaggaag
      841 agattcgggt ctgtaattga ttcgcggatg tattcttcag cattggtcgc atgacctgaa
      901 tagcttgtat ctttgatcat ctcttcagac atggcgctga tgtcatcaag ctttggtccg
      961 acaaccccga cggcactcgg aattccagag atggtatggc aggaaccgca ggctcccttt
     1021 tggaaggcgg cgagcacggc tgggtcgccg ttgctggtgg ttgaggtgtt catggcaggc
     1081 attgcggcgc cggtttcagt gacaaccaat tttccgcgca tgccaaggtc tgcatggttg
     1141 ccgacgccgt cataatagcc gatctcgcca tgtacatttg gaacagtgat ggtaatggac
     1201 gtttcttcgc ctttttcctt cacgatctca gagctggcat ttagttcagg aagagtaatg
     1261 ctatgttcgc cttcaccgcc gttgatgagg gtgatggtga ttcgttcacc cgctttggcg
     1321 ctgagcgtgg ggttttcttg cccgttgatc tcgtcgctta cgccaaggaa aaccaaattg
     1381 ccattgcgca agtcggtcgt taagacatat tctttgtcca cctgttgaaa gtctgcgcct
     1441 gcacaggcag caagaa
//
LOCUS       DAOQJE010000926         1455 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_86456_length_1455_cov_4.334286, whole genome shotgun sequence.
ACCESSION   DAOQJE010000926 DAOQJE010000000
VERSION     DAOQJE010000926.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1455)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1455)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1455
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_86456_length_1455_cov_4.334286"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..871)
                     /locus_tag="PLT08_18015"
     CDS             complement(<1..871)
                     /locus_tag="PLT08_18015"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HNE06429.1"
                     /translation="MKKNLFLAFAIIWVIACGTSVVSEESATAEAPADSAETAQVPVG
                     GEMSPEIDALMAKYVDGPGGAVMVIQDGEIIYQNGYGLADVENGLPITTDSVFHLGSV
                     GKQFTALGVMMLAEQGLVNYDDPIGNYIPELAWMSDEVTIRRLLYHTSGILGYDDSDS
                     IYDALVASSDAPTNEDLLDVLAEQGGMLTDPGEEYSYSNTGYEILGSLIERVSDEKYA
                     LFMDENIFAPLGMTHTFSLPNPERLKDKNVAQSYYLENGQPLPYEPDILDGLTGSGSI
                     YTTLGDMYLYDQAL"
     gene            1009..1374
                     /locus_tag="PLT08_18020"
     CDS             1009..1374
                     /locus_tag="PLT08_18020"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HNE06430.1"
                     /translation="MAKILIVDDDEKVTTLLARYLSALGYHVTTLNQSSKAISTAEQF
                     RPDLFILDIMMPPPDGFTLTSMLRDDVKFARTPILIITALDNSNSRATTLGANGYLAK
                     PFNLDELAAKITELVGETK"
BASE COUNT          446 a          357 c          251 g          401 t
ORIGIN      
        1 gtaacgcttg gtcatacaaa tacatatccc caagcgttgt ataaatactt ccagagcctg
       61 tcagtccatc gagaatatcc ggttcatagg gcaaaggttg tccattttcc aagtagtaac
      121 tttgcgctac attcttgtct tttaagcgtt cagggttggg caaagaaaac gtatgtgtca
      181 tacctaaagg cgcaaaaata ttctcatcca taaacaaagc atatttctca tcagaaacac
      241 gttcaattaa tgaccccaat atctcataac ccgtattact ataactatat tcctcgcctg
      301 ggtctgtaag catacctcct tgttcagcca aaacatctag caagtcttca ttcgttggcg
      361 catctgaaga tgccaccaaa gcgtcataaa tactatcgct atcgtcatat cccaaaatgc
      421 cagaggtatg atacaaaaga cgacgaatgg ttacttcatc gctcatccat gccaattcag
      481 ggatatagtt tccgatcggg tcatcataat tcaccaatcc ttgttcagcg agcatcatca
      541 cacccagtgc agtaaactgc ttacccactg agcctaaatg gaacacggaa tccgtagtaa
      601 tgggcagtcc attttcaaca tctgccaacc catagccatt ttggtaaata atctctccat
      661 cctggatgac catcacagca ccgcccggac catcaacata ctttgccatt aacgcatcaa
      721 tctctggaga catttcgcct ccaaccggaa cttgggctgt ctccgcacta tcggctggag
      781 cctccgccgt agctgattct tcacttacta cagaagttcc acaggctatt acccatataa
      841 ttgcaaacgc caaaaataag tttttcttca tatctattcc taaatactga tttgaacgcc
      901 agattataac ggaaccttaa cattgaaatt accttactaa cttattttca aaccatgccc
      961 aaggtataat tgccaaagaa aatggttcat taacaggaag tgcagacaat ggcaaaaata
     1021 ttaattgtgg atgacgatga aaaagtaact actctgcttg cccgatatct atccgcatta
     1081 ggttatcatg ttacaacgct caatcagagt tccaaagcaa tttctactgc cgagcaattt
     1141 cgcccagacc tttttattct ggatatcatg atgccccctc cagatggttt tacactcact
     1201 agtatgcttc gtgatgatgt aaaatttgcg cgcactccaa tccttatcat tacggcgttg
     1261 gataatagca atagccgagc taccaccttg ggagcaaatg gctatctggc aaaacccttc
     1321 aatttagatg aactcgccgc caaaatcact gaattggtcg gtgaaacaaa gtaatccttc
     1381 caaggaggaa ggcatggcaa aaatcttaat aatcgacgat gatgctgaaa ctataatatt
     1441 acttgagtca attgt
//
LOCUS       DAOQJE010000927         1454 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_86592_length_1454_cov_5.035740, whole genome shotgun sequence.
ACCESSION   DAOQJE010000927 DAOQJE010000000
VERSION     DAOQJE010000927.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1454)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1454)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1454
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_86592_length_1454_cov_5.035740"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(497..>1454)
                     /locus_tag="PLT08_18025"
     CDS             complement(497..>1454)
                     /locus_tag="PLT08_18025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE06431.1"
                     /translation="LPNVQMDGVSDHYTKVKRVIDTLPSGIINYLDFSLPQKLILLDS
                     VKTTFPDLGFNWEWFLKKFDYGQDESIEKREHKESIERFLIAIGMQASRIDSKEIPYE
                     LHYFRNSGDIAKHVYYTTLSSFIGIPYIPNYHRALLYKAMLTAHNNPEFAQKILAENF
                     FSGKPDITAAQAAILQYFEQNVIKPTDKLINSVLPWQGITSPMPPLLSRILTVSKKKN
                     CTILDATLSLRASAEATAFRKWCAEITEAYETSDRKSLFKLLRGFISECDRWSKDLGA
                     DRPGGQISLNFFGIGIQTEIPEPITSIQRRFTKHLVFLRDLV"
BASE COUNT          408 a          256 c          312 g          478 t
ORIGIN      
        1 aacccttgcg tagattataa atgaccaaag cctaaactgt tagattattg taacaggttg
       61 aaagtatatt ttgttcagta aaagcactcg ggtggacaca tctaaaaccc acacacgggg
      121 cgattgatag acttgtctat gaattgtatg ggttgacagc ggaggaaata gatattgttg
      181 aaggaaaata aaaccgcgta agttggtttt ataaaataaa taccgctgat aaacccatga
      241 attgtcggcg attcggtgtt attgtggagt ctggatgctg ttagctggcg cgatttttgc
      301 atttttggat aggaattatc catattcaaa cagcagtaat tacaagcaaa taaaaaaacg
      361 cccttttgca agggcgtttc gttttataag tctatttcaa tttcaaacgg gatgagacgt
      421 tttgattcct ctccgctttg ttgggcgcat ttgaaaggag attttatctt gcaatttacg
      481 ccaacctgct ttttcactaa accaaatctc gtaaaaacac cagatgctta gtgaatcttc
      541 tttggatact cgtaattggt tcgggtattt ctgtttggat tccaatgcca aagaaattaa
      601 gtgatatttg tcctccaggg cgatctgctc ctaggtcttt tgaccaacgg tcgcattcgc
      661 ttatgaagcc tcttaataac ttaaaaagcg attttctgtc cgatgtttcg taggcttcgg
      721 tgatttcggc acaccatttt cgaaatgcag ttgcctctgc ggatgcacga agactgagag
      781 ttgcgtctaa aatggtgcaa ttttttttct tagaaacagt aagaatacga gagagcaaag
      841 gcggcatagg gctagtaatg ccctgccaag gcaagacaga gttgattagt ttatctgttg
      901 gttttattac attttgttca aaatattgaa gtatcgccgc ttgcgctgcg gtaatgtctg
      961 gcttacctga gaaaaaattc tcagcaagaa ttttttgggc aaattcaggg ttgttgtgtg
     1021 ctgtaagcat ggctttgtaa agaagcgctc ggtgataatt tgggatatat ggtattccaa
     1081 taaaactgga tagcgttgta tagtaaacat gctttgcgat atcacctgaa ttgcgaaagt
     1141 agtggagttc atatggaatt tcttttgaat ctattctaga tgcctgcatt cctattgcaa
     1201 ttaaaaacct ctcaatgctc tctttgtgtt cacgcttttc aatgctctcg tcttgtccat
     1261 agtcaaactt tttcaaaaac cattcccaat taaaccccag gtctgggaat gttgttttga
     1321 ccgagtcgag cagtataagt ttttgtggta atgaaaaatc aagataattg attattccgc
     1381 taggcagagt gtcaataact ctttttactt ttgtatagtg gtcagatact ccgtccattt
     1441 gaacgtttgg taat
//
LOCUS       DAOQJE010000928         1451 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_86968_length_1451_cov_3.055158, whole genome shotgun sequence.
ACCESSION   DAOQJE010000928 DAOQJE010000000
VERSION     DAOQJE010000928.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1451)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1451)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1451
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_86968_length_1451_cov_3.055158"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(75..908)
                     /locus_tag="PLT08_18030"
     CDS             complement(75..908)
                     /locus_tag="PLT08_18030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE06432.1"
                     /translation="MKKEFIHLFTYMWKVVLRGTKDAITYYLSDWRNSIANLIVGVIS
                     GMASPIVLYRFGEVETMPNTLLIGEVGILAGIIGLLSTYLLAWLVNIICAPARLHYDL
                     EIALDKTTWKGVGISKYEFPSQSGFGVGIDIHNEKLIPIRDLKVGIYEIRKGRTIISQ
                     QTRDLPWHMNVSDMWQRIFTVRPLFNAGENGKILLANWDDHSAYLETFQDNNEQKIYL
                     DRDEKYYGKIVIVGRLQELYVSCPVYVCELTYDGNKVHLKLYDERLIYDENERPMLFN
                     Q"
     gene            complement(918..1103)
                     /locus_tag="PLT08_18035"
     CDS             complement(918..1103)
                     /locus_tag="PLT08_18035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE06433.1"
                     /translation="MSLTKICPTKRAPDAGDSVAIPSSFTRLIIFLVGRLRHPRPSAG
                     NANRSAARYGQDSELLL"
     gene            complement(1170..>1451)
                     /locus_tag="PLT08_18040"
     CDS             complement(1170..>1451)
                     /locus_tag="PLT08_18040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE06434.1"
                     /translation="HAMAAARNLKGASRYAAYAAGQAGVVAHVAAHELGAAAYAIKAV
                     RGIAPDSRIEEYGRIECEWQRAQLPIEIRDLVFDDQKMRNEICWFVFNC"
BASE COUNT          397 a          345 c          270 g          439 t
ORIGIN      
        1 gcttgggatg tgcgcagaat ccccagcgtc aggtgcacgc tttgttcggc ggcttcccag
       61 cctgttatta acatttattg attaaataac atcggtcttt cgttctcatc ataaatcaat
      121 cgttcatcat ataacttcag atgaacttta ttgccgtcat atgtaagttc gcaaacatat
      181 acagggcaac tcacatacaa ctcttgcagc ctgccaacga tgacgatttt gccataatat
      241 ttttcgtctc tatccaaata tattttttgc tcattgttgt cttgaaatgt ctcaagatat
      301 gcactatggt catcccagtt tgctaaaaga attttcccat tctctccagc attaaacaac
      361 ggtcgcacgg taaatattct ttgccacatg tcacttacat tcatgtgcca aggtaaatca
      421 cgcgtttgct ggctgattat agttctgcct tttcggattt cgtaaattcc aacttttaga
      481 tctcggatcg gtattagttt ttcgttgtga atatcgattc caacgccaaa accgctttga
      541 gaagggaatt catatttgga tattccaaca cctttccacg ttgttttatc caaggctatc
      601 tctaaatcat agtgcaatct cgcgggagca caaatgatgt tgaccaacca tgcaagaagg
      661 tatgttgaaa gtaaaccgat aattcctgcc aatataccaa cttcgccaat caataatgtg
      721 tttggcattg tttccacttc cccaaagcga taaagaacaa tgggggaggc catgccagaa
      781 atgaccccaa caatcaggtt ggcaattgag tttctccaat cggatagata gtatgtgata
      841 gcgtcttttg tccctctcaa tactaccttc cacatatacg taaacaggtg aataaattcc
      901 tttttcatat tcccattcta caacaatagt tcagaatcct gtccataacg agccgccgaa
      961 cggtttgcgt tacctgcgct ggggcgggga tggcgaagcc gtccaaccag aaaaatgata
     1021 aggcgtgtga aactgcttgg gattgccaca gaatccccag cgtcaggtgc acgctttgtt
     1081 gggcagattt ttgtaagact catttgcttt tcttttttca aacaactaca ccaaaacacg
     1141 ttaattaatg agcgacataa ataaactgat taacaattaa atacaaacca acaaatttcg
     1201 tttcgcattt tttggtcgtc aaacactagg tcacgtatct caattggaag ttgcgcacgt
     1261 tgccattcgc actcaatgcg accatattct tcaatacgac tatcaggtgc tattccacgc
     1321 actgccttaa ttgcataggc tgctgcaccc aattcatgtg ctgctacatg agcaaccact
     1381 cctgcttgac cagccgcata tgcagcatag cgtgatgctc ctttcaggtt tctggctgcc
     1441 gccattgcgt g
//
LOCUS       DAOQJE010000929         1450 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_87097_length_1450_cov_3.236559, whole genome shotgun sequence.
ACCESSION   DAOQJE010000929 DAOQJE010000000
VERSION     DAOQJE010000929.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1450)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1450)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1450
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_87097_length_1450_cov_3.236559"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1159)
                     /locus_tag="PLT08_18045"
     CDS             complement(<1..1159)
                     /locus_tag="PLT08_18045"
                     /inference="COORDINATES: protein motif:HMM:NF012428.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HNE06435.1"
                     /translation="MSDQTKTWIEKDKKQLHPTYHPKTHANPMVIEKGEGVWLHLTDG
                     RKILDGMAGLWNVSAGYGREELAKAAYDQMKDLAFTSNFSGMTNLPSIQLADKLAGFA
                     YEGLNTTLFTSGGSEANDSAFKTARYYWKRKGKPGKYKVIARKGAYHGVTIGTTFATG
                     LEKYHLMFGPAPEGYVHIPAPNPYRYEGKMKDGETVGQAAARELEEAILREGADTVAA
                     FIAEPVMGVGGVIVPPDDYFPLIRQICDKYEVLFIADEVITGFGRTGEWFALKHWNVK
                     PDILSFAKAVTSGYAPLGGIQISDEIRDTMESAGDTEAYMHGYTYSGHAMACAVGLRN
                     IEIMEQEEFPKRARELGNRLLEGLKKLEEFSFVGDVRGLGLVCGVEIVSDKT"
     gene            complement(1227..>1450)
                     /locus_tag="PLT08_18050"
     CDS             complement(1227..>1450)
                     /locus_tag="PLT08_18050"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: protein motif:HMM:NF012690.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HNE06436.1"
                     /translation="EEIEDYVAEGVEAAVPYRGKAREVLTQLVGGLQSGMSYSGAKTI
                     EEFQEKAIFVRMTGAGLRESGPHDVEVLG"
BASE COUNT          365 a          400 c          312 g          373 t
ORIGIN      
        1 tagttttatc tgaaacaatc tccaccccac agaccaaacc aagtccgcgc acatcaccaa
       61 caaaggaaaa ttcttccagc ttcttcaagc cctctaataa gcggttgccc aattcacgag
      121 cacgctttgg gaattcttcc tgctccataa tttcgatatt cctgagccca acagcacacg
      181 ccatggcatg accggaatat gtatacccat gcatgtaagc ttcggtatca cccgcagatt
      241 ccatagtatc gcgaatctca tctgagattt gaataccgcc taatggagca tatccgctcg
      301 tgaccgcttt ggcaaaagag agaatatctg gctttacatt ccaatgctta agcgcgaacc
      361 attccccggt gcgaccaaag cccgtaataa cttcatccgc aataaagagg acttcatact
      421 tatcgcaaat ctgccggatc aacggaaaat aatcatcagg tggaacgatc acgccaccga
      481 cgcccatcac aggctcggcg atgaaagcgg cgacggtatc ggctccttcg cgtagaatgg
      541 cttcttccag ctcccgagcc gcagcctgcc cgacagtttc tccatccttc attttgcctt
      601 cataacgata tggattcggc gcagggatgt ggacatagcc ttcaggcgca ggaccaaaca
      661 taagatgata tttttcaagc ccagtggcga aagtggtacc aatcgtcaca ccatgataag
      721 cgcctttgcg cgcaatgacc ttgtacttgc cgggcttgcc tttgcgcttc cagtaatagc
      781 gtgcagtttt gaaggctgag tcattggctt ctgagccgcc agaagtgaac aatgtggtat
      841 tcaagccttc ataggcgaag ccagcgagtt tatctgccaa ttgaatggac ggcaaattgg
      901 tcatgccaga aaaattagat gtgaacgcca agtctttcat ctggtcataa gcagctttgg
      961 caagttcctc acgcccatag ccagcgctga cgttccacag cccagccatg ccatcgagga
     1021 tttttcgtcc atcagtgaga tgcaaccaaa cgccttcgcc tttttcaatc accattgggt
     1081 tggcatgggt tttgggatga tatgtgggat gaagttgctt tttatctttt tcaatccagg
     1141 ttttggtttg gtcactcatg agattctcca attatgttga aaaggtataa ggttgaaacg
     1201 ttcacacctt ctaccctgct aattttctac cctagtactt ccacatcatg cggaccagac
     1261 tcgcgcaaac cagcgccggt catacgcaca aagatggctt tttcttggaa ctcttcaatg
     1321 gtcttggcac cgctataact catgcccgat tgcaaaccgc cgacgagttg cgtgagcact
     1381 tcacgcgctt tgccacgata cggtaccgcg gcttccacgc cttctgccac ataatcttcg
     1441 atctcttcgc
//
LOCUS       DAOQJE010000930         1438 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_88490_length_1438_cov_4.100506, whole genome shotgun sequence.
ACCESSION   DAOQJE010000930 DAOQJE010000000
VERSION     DAOQJE010000930.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1438)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1438)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1438
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_88490_length_1438_cov_4.100506"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..375)
                     /locus_tag="PLT08_18055"
     CDS             complement(<1..375)
                     /locus_tag="PLT08_18055"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNE06437.1"
                     /translation="MSNQPLVIEELTFKYKTRPELALENVSLELKQGELLLIAGSSGC
                     GKTTLARCINGLIPRSYRGDRSGKVLLHGKEVADLQIPEMAQVVGTLLQDPERQIVAS
                     NVFNEVAFGPENLGLPRAEIVSR"
     gene            complement(420..1436)
                     /gene="thrC"
                     /locus_tag="PLT08_18060"
     CDS             complement(420..1436)
                     /gene="thrC"
                     /locus_tag="PLT08_18060"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004795 - threonine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HNE06438.1"
                     /translation="MHRYRQYLDLPAHTQIISLNEGNTPLIPMPRLGREFGCELFIKF
                     EGLNPTGSFKDRGMTAAISEAVGRGAKTVICASTGNTAASAAAYAARAGLRSIVLIPE
                     GKVAVGKLAGAIAYGAEVIQIQGSFDDALSLVVEITQKTPIALVNSINPYRIEGQKTS
                     PFEICDELGSAPDWLCLPVGNAGNITSYWAGFKQYGKGLPRVLGVQAEGAAPLVLGHP
                     VDKPETIATAIRIGRPARGEQALEAAHESNGKIMAVSDAQILDAQRILASEGVWVEPA
                     SAAGVAGLIAETATGKLDAKGKRVVVVCTGHGMKDPSIITESFSAPQVIPAKYEALVE
                     LVGS"
BASE COUNT          365 a          399 c          330 g          344 t
ORIGIN      
        1 acggctcaca atctcagcac ggggcagacc taaattttcg ggtccgaagg caacctcatt
       61 gaatacgtta ctagcaacga tctgacgttc agggtcctgc aacagagtcc ccaccacctg
      121 cgccatttca ggaatctgca agtcggcaac ttctttgcca tgaagcaaaa ccttgccact
      181 acggtcgcca cgataactac gcggaatcaa accgttgata cagcgcgcca gcgtggtctt
      241 gccacagccg ctagagcctg caattaataa caactcgccc tgcttcagct caagagaaac
      301 attctccaag gctaattcag gacgggtttt atatttgaac gtgagttctt cgatgacaag
      361 aggttgatta gacattactt ctatatttta ctaggaaacc tagctttcat aaagattttt
      421 taactgccaa ccagttcaac caaagcttcg tacttcgcag ggatgacttg aggcgcactg
      481 aacgattccg tgataatgga tgggtctttc ataccatgac ccgtgcatac caccacaacg
      541 cgcttgccct tcgcatccaa cttgccggtg gctgtttctg caatcaagcc tgccactccc
      601 gccgctgacg ctggttccac ccacacccct tccgaagcca gaattcgttg cgcatccaaa
      661 atctgcgcat cagataccgc cataatcttt ccattggatt cgtgtgcggc ttctagcgct
      721 tgttctccgc gcgcgggtct gcctatacga atagcagtag caatcgtttc gggcttatcc
      781 acggggtgac ctaacacgag cggagcggct ccctcagctt gaacgccaag cactcgaggc
      841 aaaccttttc catattgctt gaaacccgcc cagtaagatg tgatattccc cgcattgccg
      901 acgggcaaac ataaccaatc tggagcagaa cccaattcat cacaaatttc aaaaggtgag
      961 gtcttttgtc cttcaatgcg atatggattg atcgagttta ctagagcaat cggtgttttt
     1021 tgggtgatct ccacaaccaa agatagggcg tcatcaaaag agccctgaat ttgaatcact
     1081 tccgcaccgt aggcaatagc gcctgccaac ttccctactg ccaccttacc ttcgggaatt
     1141 agcacaatgg atcgcaaacc agcccgggca gcgtaagcag cggcagacgc agccgtattg
     1201 ccagtggaag cacaaatgac ggtctttgcg ccgcgcccaa ccgcttcact gatggcggct
     1261 gtcataccgc gatctttgaa agagccggtt gggttcaagc cttcaaattt aatgaacagc
     1321 tcgcaaccaa attcacgccc caggcgcggc atcggaataa gaggagtatt cccttcgttt
     1381 agcgaaataa tttgggtatg ggcgggtagg tcgaggtatt gtctgtagcg atggatta
//
LOCUS       DAOQJE010000931         1422 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_90361_length_1422_cov_4.196781, whole genome shotgun sequence.
ACCESSION   DAOQJE010000931 DAOQJE010000000
VERSION     DAOQJE010000931.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1422)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1422)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1422
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_90361_length_1422_cov_4.196781"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(60..665)
                     /locus_tag="PLT08_18065"
     CDS             complement(60..665)
                     /locus_tag="PLT08_18065"
                     /inference="COORDINATES: protein motif:HMM:NF021485.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2202 domain-containing protein"
                     /protein_id="HNE06439.1"
                     /translation="MFKYSKKILAGLLAFVTVLAVGSNFVPAKAAAPIVLDQENTSIT
                     TGLSEEEIAGLLFMREEEKLARDVYNQLYTIWGQPIFKNIAASEQTHMDQIKILLDRY
                     GLTDPALAPGQFADPTLQALYNQLLAQGSLSLTDALNVGALIEKTDIADLQAHVAQTD
                     QADIQLVYNNLMNASYNHLAAFTGTQSQGGNGKGRGNSPRH"
     gene            840..>1422
                     /locus_tag="PLT08_18070"
     CDS             840..>1422
                     /locus_tag="PLT08_18070"
                     /EC_number="2.4.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006206 - pyrimidine nucleobase metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine phosphorylase"
                     /protein_id="HNE06440.1"
                     /translation="MRAVDIIIKKRDKGQLTAQEIDFFVRGFTNGDIPDYQASAFAMA
                     VLLNGMTNFETTDLTLSMARSGQVLDLSKIVDVAVDKHSSGGVGDKTSISVMPMVAAC
                     GLPVGKMSGRGLGFSGGTLDKLESIPGYRVNLTTDEFKAQLKEKGIVLTGQSLDLAPA
                     DGKMYALRDVTGTVPSMPLIASSIMCKKIAAGAT"
BASE COUNT          375 a          299 c          339 g          409 t
ORIGIN      
        1 cattggtcaa gaaataaaaa agaaccgcct ttttaggcgg ttctttttta tgaaaaattc
       61 taatgcctgg ggctattccc tctacctttg ccgttaccac cttggctttg tgtccccgta
      121 aaggctgcga ggtgattgta agaagcattc attagattgt tatacactaa ttgaatatca
      181 gcttggtctg tctgtgctac atgagcttgc aaatctgcga tatcggtctt ttcgattagt
      241 gcgccgacgt tcaatgcatc tgtgagagag agtgaaccct gggcaagtag ttgattgtac
      301 aaggcttgaa gggtagggtc agcaaattga ccaggggcaa gcgcggggtc ggtcagaccg
      361 tagcgatcca atagaatttt gatttgatcc atatgagtct gctcgctggc tgcaatattt
      421 ttgaaaatgg gttgtcccca aattgtataa agttgattgt atacatctcg ggcaagcttt
      481 tcttcttctc gcataaagag taacccggcg atttcctctt cgctcagacc agtggtaatg
      541 cttgtgtttt cttgatcgag aacgattggc gctgctgcct tggctgggac aaaatttgaa
      601 cctactgcaa gcacagtgac aaaggctaaa agaccagcca gaattttttt agaatatttg
      661 aacatgttta atctcctttt tgagcatcat ttgatagctc ttgatatgca cgtatgatag
      721 ttttcatgta taaaggaact gtaaaaaaac gttcaagagt ttaaaaagat gagtgccgtg
      781 atattgtctg acaaaatggg ataaaataac cgcgcaagat ttcataagga gattcttaaa
      841 tgcgcgcggt agacatcatt atcaaaaaac gcgataaggg gcaactcacc gctcaagaaa
      901 ttgatttttt tgtacgcggt tttaccaatg gggatattcc agattatcaa gcttcggctt
      961 ttgccatggc ggttttgctc aatggcatga cgaattttga aaccacagac ttaactcttt
     1021 ccatggcgcg ctctgggcaa gtgctcgatc tttccaagat cgtggatgtt gccgtagata
     1081 aacattcttc tggtggcgta ggtgataaaa ccagcatctc tgtcatgcca atggtggcgg
     1141 cttgtggttt gcccgttggc aaaatgtcgg ggcgcggact tggcttcagc ggcggcacac
     1201 tcgataaact cgagtccatt cccggctatc gcgtaaactt aacgaccgat gaattcaaag
     1261 cacaattgaa agaaaaaggc atcgttctta ctgggcaatc tctcgacctt gcgccagccg
     1321 atggaaaaat gtatgccctt cgtgatgtga ccggcacagt cccttccatg ccgttgatcg
     1381 catcgtccat catgtgcaag aagatcgctg caggtgccac ag
//
LOCUS       DAOQJE010000932         1418 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_90939_length_1418_cov_1.930301, whole genome shotgun sequence.
ACCESSION   DAOQJE010000932 DAOQJE010000000
VERSION     DAOQJE010000932.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1418)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1418)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1418
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_90939_length_1418_cov_1.930301"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            360..770
                     /locus_tag="PLT08_18075"
     CDS             360..770
                     /locus_tag="PLT08_18075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009462054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjdF family protein"
                     /protein_id="HNE06441.1"
                     /translation="MKAKLTVFFEDPYWVGVFEREDEKEYQVARAIFGCEPTDAELYE
                     FLQANYHKLEFSRSFQGEIQVERKVNFKRKQREVRKQAKENGIGTKAQQAIKQVMEES
                     KKERKQDSRQQRREEEKLKFVLKQEKKKEKQKGH"
     gene            888..>1418
                     /locus_tag="PLT08_18080"
     CDS             888..>1418
                     /locus_tag="PLT08_18080"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HNE06442.1"
                     /translation="MPLRYLALGDSYTIGESVEEAERFPNQLATLLANEGVTVDVKII
                     ARTGWTTDELWRGIQAEVVTPPYDMVSLLIGVNNQYRGRGAEEYREGFVFLLNKAIEY
                     AGGDVKRVVVFSIPDWGVTPFAYGRDTTQIANEIDAFNNINLEESNKAGVHYVDVTPS
                     SRLALNDKTLIASDGLH"
BASE COUNT          405 a          301 c          335 g          377 t
ORIGIN      
        1 cttgagcgcg aagggcagct cgccattggt gccgcctcaa ttttttatca gggtattttg
       61 gacgatatcg aaaaacatga ttacgatgtc ttctcccgcc gcgcttctct tagtgcaatg
      121 ggcaaaattg ccaaacttcc tgctttgtgg atgaagatcc gctcccttta atttgctagc
      181 atcattctcc tcttgtgtgt gcaagggcgt gactgggcag tcatgccctt gcctatttaa
      241 tgataaaatt tttccatcta gaaaaaactc catttcccac aactgaattg tccctatatc
      301 tggcgtaaag atctcgccag ccctttttgt ttctattgac catggatttg gagtgaaata
      361 tgaaagcaaa acttaccgtc ttttttgaag acccttattg ggtgggggtc tttgagagag
      421 aggatgaaaa agaatatcaa gtagcgcgtg ctatttttgg ctgcgagccg acggatgcag
      481 agttatacga gtttcttcaa gccaactatc acaagcttga atttagccgc tcctttcaag
      541 gagaaattca agttgaaagg aaggtaaact ttaaacgcaa acagcgtgaa gtccgcaaac
      601 aggcgaaaga aaatgggatc ggcaccaaag cgcaacaggc gatcaaacag gtgatggaag
      661 agtcgaaaaa agagcgcaag caggattctc gtcaacaacg ccgtgaagaa gaaaagctaa
      721 agttcgtact taagcaggag aaaaagaaag aaaaacaaaa ggggcattag gtatatctaa
      781 cgcccaatgt cttttgatat acttgcttcg tgaagaaatt cgcgatcttg ttttgtctac
      841 ttttgatggg ttgttcaacc aaatcaaccc ctacaccgga gactgtcatg cccctgcgat
      901 acttggcttt aggcgattct tataccatcg gtgaaagtgt agaagaagct gaacgttttc
      961 caaatcaact tgcgacattg ctggctaatg aaggcgtgac ggttgatgta aaaatcatcg
     1021 ctcgcaccgg ctggacgacg gatgaattat ggcgcggtat tcaagccgaa gtagtgacgc
     1081 cgccctacga tatggtttca ttgctaattg gcgtgaataa tcaatatcgc ggacgtggtg
     1141 ccgaagaata tcgcgaaggc tttgtgtttt tgcttaataa agccatcgaa tatgccggcg
     1201 gagatgtaaa acgagtcgtt gttttctcaa ttccggattg gggcgtcact ccgtttgcat
     1261 atggcagaga cactacccaa atcgcaaatg agatcgacgc ctttaacaac attaatcttg
     1321 aagagtcaaa taaagccggc gttcactatg tggatgtaac ccctagttct cgattggcgc
     1381 tcaacgataa aaccttgatt gcgtccgacg gattgcac
//
LOCUS       DAOQJE010000933         1415 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_91210_length_1415_cov_8.449265, whole genome shotgun sequence.
ACCESSION   DAOQJE010000933 DAOQJE010000000
VERSION     DAOQJE010000933.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1415)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1415)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1415
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_91210_length_1415_cov_8.449265"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1415
                     /locus_tag="PLT08_18085"
     CDS             <1..>1415
                     /locus_tag="PLT08_18085"
                     /inference="COORDINATES: protein motif:HMM:NF024583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase dimerization/phospho-acceptor
                     domain-containing protein"
                     /protein_id="HNE06443.1"
                     /translation="RTPQEALGRSAVDIDLWSNPEERARFVKDLLEEGSLENVEVEFS
                     GKSGQSKLALGYYELLDIKGQRCILCMFYDVSVQRQAQSDLKESERRTRAIISSIPDM
                     IFEISKEGDFLDFMASAELVPIMEPHEFIGKNIKNMFPPRIGEQTMFALDRALVTGQV
                     HAFEYGLPPGEETQFFEARVAPVTSESAIIMVRDISQRKWVETEREKLINELEEKNSE
                     SEALREGMTILVETLDETKAVSLILEQLEKVLPFDSASVQLLKGDTLEIVSTRHLEPK
                     EMHLGTRYVVSEKEPSYPLLLGKVPYVLVKDVRATFTDLFAVAPYTLIRTWLAVPLRI
                     KRQIIGIITLDGYQPNKFTQRHAELALTYASQVAVALDNARLFSELQVELSARKNLIE
                     ELEVKNAELERFTYTVSHDLKSPVITIRGFLGFLEQDALSGNVTRLRGDIQRIADATD
                     KMQTLLNELLELSRIGRLVNPP"
BASE COUNT          383 a          326 c          329 g          377 t
ORIGIN      
        1 cgcacccctc aagaagctct cggacgttcc gctgtcgata tagacctctg gtcaaatcca
       61 gaagaacgtg cccggtttgt aaaagacctg ctagaagaag gctctcttga aaatgtagaa
      121 gtggaatttt ctggaaaaag tgggcaaagt aaattagcac ttggctatta tgaattgctc
      181 gatattaagg gacagcgctg tatcttatgc atgttctatg atgtctctgt gcagcggcaa
      241 gcacaaagtg atttgaaaga aagtgaacgc cgtacacgcg ccataatttc atcgattcca
      301 gacatgattt ttgaaatctc aaaagagggt gattttttgg atttcatggc ttcagcagaa
      361 cttgttccta tcatggaacc gcatgagttc attggcaaaa atatcaaaaa tatgttcccg
      421 cctcgtattg gcgagcagac tatgtttgct ctagaccgtg cgcttgtcac gggacaagtc
      481 catgcctttg aatacgggtt gccccccggc gaagagactc aattctttga agcccgtgta
      541 gcgcctgtca cttctgagtc tgccatcatt atggtgcgtg atattagtca acgtaaatgg
      601 gtggaaaccg aacgcgaaaa gttgatcaac gaactggaag aaaagaactc tgaatcagaa
      661 gctttacgag aaggcatgac cattctcgtt gagactttgg atgaaaccaa agccgtcagc
      721 ttgattcttg aacagcttga aaaggtactt ccatttgatt cagcttctgt gcaacttcta
      781 aaaggcgata ctttagaaat cgtgagtacg aggcaccttg agcctaaaga gatgcatttg
      841 ggcacgcgct atgtggtcag cgagaaagaa ccgtcttatc cgcttttatt gggcaaggtt
      901 ccttatgtgc ttgtgaagga cgtacgcgcg accttcactg atctgttcgc agtggcaccc
      961 tataccttaa tccgcacctg gcttgcagtt cctttgcgca tcaaacgcca gattatcggc
     1021 atcatcacct tggatggata tcagcctaac aaatttactc aacgccatgc cgaacttgca
     1081 ttaacctatg ccagtcaagt agcagtggct ttagacaatg ctcgcctgtt cagcgaatta
     1141 caagtggagt tatctgcccg caagaacttg atcgaagaac ttgaagtgaa aaatgccgag
     1201 ttggaacgct ttacctacac tgtctctcat gaccttaaat ctccggtcat taccattcgc
     1261 ggctttctcg gctttctgga acaagatgcc cttagtggaa atgtcacccg gttgcgtggg
     1321 gatattcaac gcattgcaga tgccaccgac aaaatgcaaa cccttttgaa tgaattgctg
     1381 gaactttcac gcatcgggcg cttggtgaac ccgcc
//
LOCUS       DAOQJE010000934         1409 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_91996_length_1409_cov_3.042836, whole genome shotgun sequence.
ACCESSION   DAOQJE010000934 DAOQJE010000000
VERSION     DAOQJE010000934.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1409)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1409)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1409
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_91996_length_1409_cov_3.042836"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            127..351
                     /locus_tag="PLT08_18090"
     CDS             127..351
                     /locus_tag="PLT08_18090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE06444.1"
                     /translation="MDEKQFKMLMDELTEIRNMLILIASKSGANSIDISKVLNTSDSR
                     VRQILTGAGGKKKKGVNAEKQESNNLQGEK"
     gene            353..616
                     /locus_tag="PLT08_18095"
     CDS             353..616
                     /locus_tag="PLT08_18095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE06445.1"
                     /translation="MTDKTHLDEVNRKLDILLKLMAYQTVQKMTLSEGAPLLRRLGFN
                     SAEIAEIYDSTSNAVNVRLAEAKKKKKDKQQKGGKIDTPSSQA"
     gene            693..1340
                     /locus_tag="PLT08_18100"
     CDS             693..1340
                     /locus_tag="PLT08_18100"
                     /inference="COORDINATES: protein motif:HMM:NF037501.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Swt1 family HEPN domain-containing protein"
                     /protein_id="HNE06446.1"
                     /translation="MTTDEAVYIIAHIEGVNLAKYLPLETLDRVRSLIPREIKPSPIA
                     PQVKITNKKPKSRKTIASYPLVNNAFIQRAVTIGDESFPQIVVMENSIRALIEKTLST
                     INPNWWVALVPPNVQRNVQRTIDKEKKFPYREKRGNRPILYCNFADLKEIIVANYPEF
                     RNVIIDIEWFKAKMDEVYMARNNLAHSVLLSDDDISRIALFYRDWARLLDTAGVK"
BASE COUNT          474 a          281 c          305 g          349 t
ORIGIN      
        1 ccaagcagtt tcacacgcct tagccttttt ctggctggac ggcttcgccg tccccgcccc
       61 agcgcaggta acgcatgccg ttcggcggtt tcttccaaaa taatcaggtg ataggagata
      121 aataaaatgg atgaaaagca attcaaaatg ttgatggatg aacttacgga aatcagaaat
      181 atgctaatac tgattgcctc aaaatctggc gcaaactcaa ttgatattag caaggttttg
      241 aatactagtg acagtcgtgt tcgacaaata ctcactggtg caggtggaaa gaaaaagaaa
      301 ggcgtaaacg ccgaaaagca ggaaagcaac aatttgcagg gagaaaaatg aaatgacaga
      361 caaaacccat cttgatgaag taaatcgcaa gttagatatt ttgctcaaac ttatggcgta
      421 tcagactgtt cagaaaatga cactttcaga aggtgcgcca cttctccgca ggcttggttt
      481 taattcagca gagatagcag aaatctacga tagcacgtca aatgccgtga atgtgcgttt
      541 ggctgaggca aagaagaaaa agaaagacaa acaacaaaaa ggcggaaaaa ttgatacacc
      601 gtcaagtcag gcatgaactt ctaaacaagt tgaaggtaac tcgccaagcg ttgagtcttc
      661 gggctaaaca aataaaagcc aaatatggac cgatgaccac cgatgaagca gtttatataa
      721 ttgctcacat cgaaggcgta aaccttgcaa aatatttacc gcttgaaact cttgaccgag
      781 ttcgttcttt gattccaaga gaaattaagc catcgccaat tgcaccccag gtaaagatta
      841 caaacaagaa acctaaaagt cggaaaacga ttgcttctta cccattggtg aacaatgcct
      901 ttattcagcg agccgtcaca attggtgatg aatcttttcc tcaaattgtt gtgatggaaa
      961 attctattcg agcattgatt gagaaaacac tttcaacaat caatcccaat tggtgggttg
     1021 cccttgtgcc gccaaacgtg caaagaaatg ttcagcgaac aattgacaaa gagaaaaaat
     1081 ttccctaccg tgaaaaacgg ggaaaccgcc caatactcta ttgtaacttt gccgatttga
     1141 aggaaattat tgtcgctaac tatcctgaat ttcggaatgt aatcattgat attgaatggt
     1201 tcaaggcaaa aatggatgaa gtctatatgg caagaaacaa tcttgctcat agtgttctct
     1261 tgagtgatga tgatatttca cgcatagccc tgttttatag agattgggca aggttgcttg
     1321 ataccgcagg tgtaaaataa gacgaaaacc gccgaacaaa gcgtgcacct gacgctgggg
     1381 attctgcggc aatctcaggc agttttcta
//
LOCUS       DAOQJE010000935         1402 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_92907_length_1402_cov_4.086117, whole genome shotgun sequence.
ACCESSION   DAOQJE010000935 DAOQJE010000000
VERSION     DAOQJE010000935.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1402)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1402)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1402
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_92907_length_1402_cov_4.086117"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1192)
                     /gene="ftcD"
                     /locus_tag="PLT08_18105"
     CDS             complement(<1..1192)
                     /gene="ftcD"
                     /locus_tag="PLT08_18105"
                     /EC_number="2.1.2.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR02024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030409 - glutamate formimidoyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0030412 - formimidoyltetrahydrofolate
                     cyclodeaminase activity [Evidence IEA];
                     GO_process: GO:0019556 - histidine catabolic process to
                     glutamate and formamide [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate formimidoyltransferase"
                     /protein_id="HNE06447.1"
                     /translation="MTNQLIECIPNFSEARRPEVIDQIVAAIQAVGEVKLLDRSSDLD
                     HNRTVLTFAGSAAGVEEAAFRAIQTASELIDLDQHTGEHPRIGATDVVPFVPLSGASM
                     EECIAIAKRLAERVGKELNIPVYLYEAAATKPERTNLENIRKGQYEGLKTEIEKPERQ
                     PDFGPNKLPKAGATVIGARNPLIAFNVYLTTDDVSIAKKIAKAVRQSSGGLRYVKGLG
                     LLVEGRAQVSMNLTNFRETPIARVVEFIRREAERYGVGIHHSELVGLIPQEALVDAAV
                     WYTQLDAFSPEQVLESRLFSTPTASASNALKPASFIEELAAPTPTPGGGSAGAYAGAM
                     GAALVAMVAGVTIGKKKYAEVEAEMQAIRVVAENLRKELTQAVDDDAASFEVLMAKFK
                     MPKET"
BASE COUNT          307 a          350 c          406 g          339 t
ORIGIN      
        1 cggtctcttt cggcattttg aattttgcca tgagcacttc aaaggatgcc gcatcgtcat
       61 cgacagcttg agtaagctct ttacgaagat tctctgccac cacacggatg gcttgcattt
      121 cagcttcgac ttcggcgtat ttcttcttgc caatggtcac acctgccacc atggcaacca
      181 aggcagcgcc catggcaccc gcataagccc ccgcggaccc gccgccgggc gtcggggtcg
      241 gcgcggctaa ttcttcgatg aaagaagcag gcttgagcgc gttggaggcg gaagcggtgg
      301 gagtagaaaa cagcctggac tcgagaacct gttcgggaga gaaagcatcc aactgggtgt
      361 accaaaccgc tgcatccaca agggcttctt gcggaattaa accgaccagt tcgctgtgat
      421 gaatgccgac gccataacgt tcggcttcgc ggcgaatgaa ttcgaccaca cgcgcaatcg
      481 gcgtttcgcg gaagttggta aggttcatcg aaacttgcgc gcgcccttcc acaagcagac
      541 caagaccttt gacgtaacgc agaccgcccg aagactgacg cacggctttg gcaatctttt
      601 tcgcgatgct gacatcatcg gtcgtgagat agacgttgaa agcaatcagc ggattgcgtg
      661 ccccgataac ggtggcaccc gccttgggaa gtttattagg accaaaatct ggctggcgtt
      721 caggtttttc gatttcggtc ttcaagcctt cgtattgccc tttgcggatg ttctcaaggt
      781 tggtacgttc gggtttggta gcggcagctt cgtaaagata aacaggaata ttgagttctt
      841 tgcccacgcg ctcagcaagg cgtttggcga tggcaataca ttcttccatg cttgcgccac
      901 tgagcggaac aaaaggcacc acatcggttg cgccgatgcg cggatgttcg ccggtgtgct
      961 ggtcgaggtc gatcagttcg gaggcggttt gaatggcacg gaaggcggct tcttctacgc
     1021 ctgcggcaga cccggcaaag gttagcacgg tgcgattgtg gtcgaggtca gaggagcggt
     1081 cgagcagttt cacctccccc acggcttgaa tggcggcgac gatctggtcg atcacttcgg
     1141 ggcgacgcgc ttcggaaaag ttgggaatgc attcaatgag ttgattggtc atggagtttg
     1201 atagtctcgc agtttcgtta gttgggtatg catccattat aaaagatatt caggagaaaa
     1261 aaaacaacct caccgcttgg tgaggttgtt tcggtctttt gattgattta tggcatccaa
     1321 gacatcatgg tgatcttgcc gtcggtgaca aaggcttggc agtaggaata ggtggtgccg
     1381 tcgctggctt tgaccttgaa ct
//
LOCUS       DAOQJE010000936         1398 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_93469_length_1398_cov_5.673120, whole genome shotgun sequence.
ACCESSION   DAOQJE010000936 DAOQJE010000000
VERSION     DAOQJE010000936.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1398)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1398)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1398
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_93469_length_1398_cov_5.673120"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1384)
                     /locus_tag="PLT08_18110"
     CDS             complement(<1..1384)
                     /locus_tag="PLT08_18110"
                     /EC_number="2.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012670.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016744 - transketolase or transaldolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HNE06448.1"
                     /translation="MAIAGAHLAATFNKPGHELLDAYIYAIVTDGDLMEGVASEAASL
                     AGHLSLGKLIYLYDDNKISIDGSTDLSFTEDRAKRFEAYGWHIQRVEDGNDVEAIDAA
                     IKAAKTDPRPSLILCRTIIGFGAPNRQGTQKAHGEPLGDEELNAAKDNLGWPKEPRFL
                     IPDDVLEFYRKAVDRGRELEADWKMRREAYARLFPDLGTEFNRRILMNKLPEGWESAL
                     PTFPADPKGMATRAASGKVINALVAKLPELIGGSADLAPSNNTKIDGSPAFQKDSPAG
                     RNFHFGVREHGMGSVLNGMAIFGGVIPYGATFLVFADYMRASVRLAALSHYPTIFIFT
                     HDSVGLGEDGPTHQPVEHLASLRIIPNLVVLRPADANETAQAWKAAIARRDGPTVLAL
                     TRQNVPTMDGPALVEKGAYVLKDFGTPEIILMASGSEVGLVMDAAQKLADEGKGVRVV
                     SFPSWELFEKQ"
BASE COUNT          359 a          365 c          349 g          325 t
ORIGIN      
        1 cctgtttctc gaacaattcc caactcggga aggacaccac acgcacaccc ttgccctcgt
       61 ccgctagttt ttgtgcggca tccattacca gcccaacttc tgagccagat gccatgagaa
      121 taatttcagg ggtgccaaag tctttcaaga cataggcgcc tttttcaacc aatgcaggac
      181 catccattgt aggaacattt tggcgggtca atgccaagac agtaggacca tcacggcgag
      241 caatggcagc tttccacgct tgagccgtct cattggcatc cgctgggcga agcacaacaa
      301 gattcggaat aatacgcaaa gaagccaaat gctccaccgg ttgatgtgtc ggtccatctt
      361 cgcccaaacc gacagagtcg tgagtgaaaa tgaaaatggt cgggtaatgt gaaagagccg
      421 ctaaccgcac agaggcacgc atgtaatcgg caaagaccag gaaggtcgcg ccgtacggaa
      481 tcacgccgcc gaaaattgcc ataccattta ggacagaccc catcccatgt tcgcgcacac
      541 cgaagtggaa gttacgtcca gccggagagt ctttttggaa agcaggcgaa ccgtcaattt
      601 tggtattgtt cgaaggagca aggtctgccg agccgccaat aagctcaggg agttttgcca
      661 ccagtgcgtt aataacctta ccagaagcgg cacgagttgc catacctttc gggtcagcgg
      721 ggaatgtcgg tagtgcagat tcccaacctt cgggcaattt gttcatcaat atgcggcgat
      781 taaattctgt tccaagatcc gggaagagcc gtgcatacgc ttcgcggcgc atcttccaat
      841 cggcttcgag ttcacgtccg cggtcaacgg ctttgcgata aaactcaagc acatcatcag
      901 ggatcaagaa acgaggttcc ttgggccagc caaggttatc ttttgcagca ttcagttctt
      961 catcacctag cggttcgcca tgtgctttct gagtgccttg tcggttcgga gcaccaaaac
     1021 cgatgatggt gcggcacaaa atcaacgacg ggcgtgggtc tgttttagca gctttgatag
     1081 cggcatcaat cgcttctaca tcattaccat cttctacacg ctgaatatgc cagccatatg
     1141 cttcaaaacg cttggcgcgg tcttctgtga aggaaaggtc tgttgagccg tcaatggaaa
     1201 tcttattatc gtcgtacaga taaataagtt ttccgagcga gaggtgccca gccagtgaag
     1261 cggcttcaga agcgacacct tccatcaaat caccatctgt gacgatggca tagatgtatg
     1321 cgtcaagtaa ttcatgacct ggtttgttga atgtggcagc taagtgtgca ccagcaattg
     1381 ccatccccac accagtgg
//
LOCUS       DAOQJE010000937         1398 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_93524_length_1398_cov_2.737900, whole genome shotgun sequence.
ACCESSION   DAOQJE010000937 DAOQJE010000000
VERSION     DAOQJE010000937.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1398)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1398)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1398
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_93524_length_1398_cov_2.737900"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1284
                     /locus_tag="PLT08_18115"
     CDS             <1..1284
                     /locus_tag="PLT08_18115"
                     /inference="COORDINATES: protein motif:HMM:NF019149.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HNE06449.1"
                     /translation="AWVAQMELDGMKERMTMGVKARLKSGKANTGQDRYGYQRQGDQI
                     VLVEEEAFWVRKIFEWYLDGCRLMEIRDRLIAGGAPQKGSSVPRRIHWARSSIQAILK
                     SAKEYAYGLKIQSRKGELFEIPVEPIIDIATYDSFREMRARKKTYPARRVHHDYLLGG
                     VLKCDCGNKWGARTQKTRKNRKGVLVERKTLTGVYFCRQNHKDLVSPDCPRHIGAKKA
                     DDEAWEKICRALDKPEYLLSQAMDLVDQLRANASNLHEERDRIEKQLQRINHNRQWVI
                     TQAREGRFTNNDMEQQLATLTLQEISYKRDLSALGQTININALDNWEEKFEEYLADLQ
                     VGVVELKEAAPQTEEERHRVFLLKKKVVDTLVEEVTINRNRELNVVIRLDLQKILDQD
                     AGLENLSPAAYSRRGEIYTRKQSSHVHHHHYASCE"
     gene            complement(1193..>1398)
                     /locus_tag="PLT08_18120"
     CDS             complement(1193..>1398)
                     /locus_tag="PLT08_18120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="OB-fold domain-containing protein"
                     /protein_id="HNE06450.1"
                     /translation="NAPYTVAIVKLDEGPMVTAQLTDVDNSEVQIGMPVEMVTRKMRN
                     DGDERGLIVYGYKFRPAWNMPQG"
BASE COUNT          419 a          274 c          375 g          330 t
ORIGIN      
        1 gcttgggtgg ctcaaatgga attggatggc atgaaggagc gcatgacaat gggcgtgaaa
       61 gcccgcctga aatcgggaaa ggcaaacact ggacaggatc gttacggata ccagcgtcaa
      121 ggcgatcaga ttgtgttggt tgaggaggag gcgttttggg ttcggaagat atttgaatgg
      181 tatcttgatg ggtgtcgtct catggagatt cgagacaggt tgattgccgg gggcgcaccc
      241 caaaagggga gcagcgttcc aagaaggatc cactgggctc gctccagtat tcaggcaatc
      301 cttaagtctg ccaaggaata tgcttatgga ttgaaaattc agtcgagaaa aggagagctt
      361 tttgaaatac cggtggagcc aattattgat attgcaacct atgatagctt tagggaaatg
      421 agggcacgaa aaaaaacata tcctgctcgg cgtgttcacc atgattacct gcttggcggg
      481 gtactaaaat gtgactgtgg caataagtgg ggcgcgagaa ctcaaaagac cagaaagaat
      541 cggaaaggtg ttctggtcga gcgaaaaaca ttaacaggcg tttacttttg tcgccagaat
      601 cacaaagatc ttgtttcccc cgattgccca agacatatcg gcgctaaaaa ggctgatgat
      661 gaagcttggg aaaagatatg ccgcgctctc gacaagcctg aatacctgct ttcacaagcg
      721 atggatttgg ttgatcaact tcgtgcaaat gcctctaatc ttcatgaaga aagggacaga
      781 atcgaaaaac agcttcaaag gatcaatcac aaccggcaat gggtgattac ccaagcaaga
      841 gaaggaaggt tcacaaacaa cgatatggaa caacaattgg cgactttgac cttgcaggag
      901 ataagttata agcgcgacct gtctgcatta ggtcagacga taaatatcaa tgcgctcgat
      961 aactgggaag aaaaatttga ggagtacctg gcggatttac aggtaggggt tgtagagtta
     1021 aaagaagccg ctcctcaaac tgaagaggag cgacatagag tattcttact caagaaaaag
     1081 gtcgtagata ccttggttga ggaggtgaca atcaacagaa atcgagaact aaatgttgtg
     1141 attcgcttgg atttacaaaa gattctcgat caagatgcag gtttggagaa ccttagccct
     1201 gcggcatatt ccaggcgggg cgaaatttat acccgtaaac aatcaagcca cgttcatcac
     1261 catcattacg catcttgcga gtgaccattt caacgggcat tccgatctgg acttcggagt
     1321 tgtctacatc ggtcagttga gctgtcacca tgggaccttc atctagtttt acgattgcca
     1381 cggtgtaagg ggcatttg
//
LOCUS       DAOQJE010000938         1397 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_93609_length_1397_cov_8.052906, whole genome shotgun sequence.
ACCESSION   DAOQJE010000938 DAOQJE010000000
VERSION     DAOQJE010000938.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1397)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1397)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1397
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_93609_length_1397_cov_8.052906"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..866)
                     /locus_tag="PLT08_18125"
     CDS             complement(<1..866)
                     /locus_tag="PLT08_18125"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HNE06451.1"
                     /translation="MRSDTVKKGYEKAPHRALLRATGLKDEDFNKPFVAIVNSYVDVV
                     PGHVHLQEFGKLVKEAVRAAGCVPFEFNTIGVDDGIAMGHMGMKYSLPSRELIADCVE
                     TMIEAHRFDAMVCIPNCDKIVPGMLLAAMRVNVPTIFVSGGAMKAGMTPDGEAIDLIS
                     VFEGVGAFSAGKIDEKRLTLLEKFACPSCGSCSGMFTANSMNCLMEVLGLALPFNGSA
                     LAKTPEREELARKAAAQIMTLIERDIKPRDIVTAEAIDDAFALDMAMGGSSNTVLHTL
                     ALANEAGVDYPLT"
     gene            complement(1149..>1397)
                     /locus_tag="PLT08_18130"
     CDS             complement(1149..>1397)
                     /locus_tag="PLT08_18130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HNE06452.1"
                     /translation="EKNSQLVRSFLSGIRSQGGEPRFVYKTGTADLNIVAPVWQCPAV
                     VYGPGDSALDHTPNEHINLDDYQKAVHALSATLEKLTG"
BASE COUNT          353 a          386 c          316 g          342 t
ORIGIN      
        1 gtcagcggat aatccacacc ggcttcgttg gctaatgcca atgtgtgcaa cacggtatta
       61 gatgacccgc ccattgccat atccaacgcg aaggcatcgt caatggcttc ggcggtgacg
      121 atatctctcg gcttgatgtc gcgttcgatc aaggtcatga tctgagccgc ggcttttctc
      181 gccaactctt cacgttcggg agtcttcgct aaagccgaac cgttgaaggg caatgccaat
      241 cccagcactt ccatgagaca gttcatggaa ttggcagtaa acatcccgga gcaagagcca
      301 caggaagggc aagcaaattt ctccagcaga gtgaggcgct tttcgtcgat cttgcccgct
      361 gaaaatgcac ccacaccttc aaagacggag atcaagtcga tggcttcgcc atcaggggtc
      421 atgccagctt tcattgcgcc gcccgaaacg aagatggtcg gcacgttgac gcgcatagca
      481 gccaacaaca tgcccggcac gatcttgtcg cagttcggga tgcagaccat cgcatcaaaa
      541 cgatgcgctt cgatcatcgt ctccacgcag tcggcgatca attcacggga aggaagcgag
      601 tacttcatcc ccatatgacc catcgcaatg ccatcatcca caccaatggt gttgaactcg
      661 aacggcacac atcccgccgc acggacggct tctttcacca gcttgccaaa ctcttgcaaa
      721 tgcacgtgac cgggaacaac atcaacataa gaattaacaa tcgcaacgaa cggcttgtta
      781 aaatcctcgt ccttcaaacc ggtagcacgt aacaatgctc ggtgaggcgc tttttcgtac
      841 cctttcttga ctgtatctga tcgcatgact cttttcctgt aatagttgat ataaaaagag
      901 ccgcccgtgg ttagggcggc tctcgttttc actcggtgtt tattttgctc tcaccgtcgc
      961 catcctaacc gaaaattgtc ctttaataat aaggactaca aggacgacaa taataaggga
     1021 gaggctgtta aacatgctca tggcttttga cttcgcccga ttataggcag aaacggaaat
     1081 tcgtcaaggt cttgcatttc cgaatcggcg aatattatcg attacatttt taccttctgg
     1141 cacacttctt aacccgtcaa tttttccaac gttgcactca aagcatgaac tgctttttga
     1201 taatcatcca agttgatatg ctcattcggt gtgtgatcca atgccgaatc accgggacca
     1261 tacaccacag ccggacattg ccacaccggg gcgacgatgt tcaagtcagc ggttcctgtt
     1321 ttgtagacga aacgcggctc accgccctgc gacctgattc cacttaagaa gcttcgtacc
     1381 agttgagagt tcttctc
//
LOCUS       DAOQJE010000939         1395 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_93901_length_1395_cov_9.565672, whole genome shotgun sequence.
ACCESSION   DAOQJE010000939 DAOQJE010000000
VERSION     DAOQJE010000939.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1395)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1395)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1395
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_93901_length_1395_cov_9.565672"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(3..416)
                     /locus_tag="PLT08_18135"
     CDS             complement(3..416)
                     /locus_tag="PLT08_18135"
                     /inference="COORDINATES: protein motif:HMM:NF024247.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin family protein"
                     /protein_id="HNE06453.1"
                     /translation="MTHIITSLCVRDRGCIEVCPVECIVPGNPADGFPMVYIDPDTCI
                     DCGACVPECPFSAIFPEDEVPSSYTAKGTEYINRVGLSGHYEASNHHGKPVVLETTKK
                     LDAGETVDLTPDTQLNYAFFKSGPGYSAKDTDSGS"
     gene            complement(537..1139)
                     /locus_tag="PLT08_18140"
     CDS             complement(537..1139)
                     /locus_tag="PLT08_18140"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002387688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HNE06454.1"
                     /translation="MAFELPKLAYAYDALEPHIDARTMEIHHTKHHQTYITNLNGAVE
                     KTPELQGKSLEALLSDLNAVPEGVRAVIRNHGGGTYNHNLFWEIMGPNAGGAPKGDLA
                     KAIDASFNSFDAFKDEFTKAATTRFGSGWAWLVKKGSGLAVVSTANQDTPLSDGLTPI
                     LGIDVWEHAYYLNYQNRRPDYITAFYNVINWEAVSAKFAA"
BASE COUNT          348 a          309 c          388 g          350 t
ORIGIN      
        1 gactagcttc cgctatcggt gtctttggcg ctgtagccag gaccactctt gaagaaggcg
       61 tagttgagct gggtatcagg agtgagatcg acggtttcac cagcatcgag cttcttggtg
      121 gtttcgagaa caaccggctt gccgtggtga ttggaagctt cgtagtgacc agagagacca
      181 acacgattga tgtactctgt gcccttggca gtgtaagatg aaggaacttc atcttcaggg
      241 aagatggcac tgaaaggaca ttcgggcacg caagcgccgc agtcgatgca ggtgtcagga
      301 tcgatataga ccatggggaa gccatcagca ggattgccgg ggacgataca ttcgacgggg
      361 caaacttcga tgcatccgcg gtcacgaacg cacaaactgg taataatatg agtcatttct
      421 tttctccttg tgggatatag acaaacgatt tcttcctatt ataactttgt ttacgtgagt
      481 gacaaggaca aaaaaacacc ccgtattgct acggggtgtt ttttacatcg tacgtactaa
      541 gcagcaaatt ttgcagagac agcttcccaa ttgattacat tgtaaaacgc ggtgatgtaa
      601 tcggggcggc ggttttgata gttcaagtag taagcgtgtt cccacacgtc gataccgaga
      661 atgggagtga gaccgtcaga aagcggagtg tcttgattgg ctgtggagac aacggcgagt
      721 ccgctgccct tcttgacgag ccaagcccag ccggagccga agcgagtggt ggcggctttg
      781 gtgaattcat ccttgaatgc atcaaaggag ttaaaggaag catcaatcgc cttggcgagg
      841 tcacctttgg gggcgccgcc ggcgttgggt cccataattt cccagaaaag gttgtgattg
      901 taggtgccgc cgccatgatt gcggataacg gcgcgaacac cttcaggaac tgcgttcaaa
      961 tcgctgagca aggcttcgag ggatttgccc tgaagttcgg gggtcttttc aacagcgcca
     1021 ttgaggttgg tgatataggt ctgatggtgc ttggtgtgat ggatctccat cgtgcgtgca
     1081 tcaatgtggg gttcaagcgc atcgtacgcg tatgcaagtt tgggaagttc aaaagccata
     1141 atagttctcc ttcgagttga ttatttgagt tggtctttcc attgtatccc gcccgtgggc
     1201 tgggcgcaag ataaaataaa ttttattaca aatatctaat aaatctaaag gcaaaaaaag
     1261 ccgccgtaat ttcttacgac ggcatttcta caaattactt cttatcgcca ccaagcaagc
     1321 cgccgagcag acctgcggcg tcgtcgagac cgatgccatc gcttaggtca acgtttccgg
     1381 cgagaatggc atcaa
//
LOCUS       DAOQJE010000940         1395 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_93904_length_1395_cov_5.678358, whole genome shotgun sequence.
ACCESSION   DAOQJE010000940 DAOQJE010000000
VERSION     DAOQJE010000940.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1395)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1395)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1395
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_93904_length_1395_cov_5.678358"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            360..1190
                     /locus_tag="PLT08_18145"
     CDS             360..1190
                     /locus_tag="PLT08_18145"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HNE06455.1"
                     /translation="MHRERVSENVFWFQSEVYAQVTAGVIIGPQWAVVIDTLALPEET
                     LSMREFIEHELGVQVRYVINTHYHADHCWGNCFFPGAVVIGHSKCRQYLVEDGAESLE
                     NAQKQNPNLRQVKIVPPHITFETGEMTLRVGKKNLIISTSPGHSDDGISVLVEEDRVL
                     FAGDAFMPLPFIVDGDADDMLNSIKKIGKMGLENIIQGHGDIILRGEIDAAVRENVNY
                     LNNIKKAVKSGGKRKNADEYLEEIAIEDCGKSRVYLGGLAETLHRRNLRALHRQMFGE
                     "
BASE COUNT          382 a          335 c          345 g          333 t
ORIGIN      
        1 caggcatagg gctagccgga agctgtgtag cggtcggagc aggcgtaggt gtgacagtag
       61 cggtgggcgg caatggggta aaagtcgccg ttggctcggg ggtagccgca ctacatgata
      121 tttggaacag gatcaacaaa acaacagaaa gttttcgaac catctagaat cctccttttg
      181 ggtgaaggga caggcacaag tcttgtcaga atataactca aatctgaaga aatctccata
      241 tttgtctata catcttgaaa tttttaagat tgtatgctat taacaacgaa tactaaacac
      301 aaatggcttc gccccagtat gggatagcaa acaggcgaac cagtgaagaa ggtaaaacta
      361 tgcaccgcga acgagtttct gaaaacgtat tttggttcca aagcgaagtc tacgcgcagg
      421 tcactgcggg tgtcattatt ggtccgcaat gggcagttgt catcgatacc ctcgctctcc
      481 ccgaagagac cctctccatg cgcgaattta tcgagcacga attgggcgta caggtccgtt
      541 atgtgatcaa cacccattat cacgccgacc actgctgggg taactgtttc ttccccggcg
      601 ctgttgtgat cggtcattcg aaatgccgcc aatatctggt ggaagatggc gctgaatcat
      661 tggaaaatgc tcaaaagcaa aaccctaatc tgcgtcaggt taagatcgtg ccgccgcaca
      721 tcacctttga aaccggcgaa atgaccctac gtgtgggtaa gaaaaatctc atcatctcca
      781 cttcccccgg tcacagtgac gatggcattt ccgtcctcgt cgaagaagac cgtgtgctct
      841 tcgccggtga tgctttcatg ccattgccct tcattgtaga tggcgatgcc gatgacatgt
      901 tgaactccat taagaagatc ggcaagatgg gcttggaaaa tatcatccaa ggtcatggcg
      961 atatcatcct gcgaggtgag atcgacgccg ccgtacgtga gaatgtcaac tacctcaaca
     1021 atatcaaaaa agcagtcaag tcgggtggca aacgcaagaa tgccgatgaa tatctcgaag
     1081 agatcgccat tgaagattgt ggcaagagcc gtgtctatct cggcgggctg gcagagaccc
     1141 tgcacaggcg caacctgcga gccttacacc gccaaatgtt cggagagtaa acgaatacac
     1201 tagatgttgc agatgcaagc cgggtctgca acatctttgt tagtggctag agccttatac
     1261 attatgacaa gaggattaac aacatgaaat ttttactgga acgttcacgc tacctaactt
     1321 tgatcggcat catctctctg ttgatcgctt ctttcgccgc ctttgcctgg ggcaccatca
     1381 aaacagtgac tacta
//
LOCUS       DAOQJE010000941         1392 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_94367_length_1392_cov_2.985041, whole genome shotgun sequence.
ACCESSION   DAOQJE010000941 DAOQJE010000000
VERSION     DAOQJE010000941.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1392)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1392)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1392
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_94367_length_1392_cov_2.985041"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(83..610)
                     /locus_tag="PLT08_18150"
     CDS             complement(83..610)
                     /locus_tag="PLT08_18150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE06456.1"
                     /translation="MLDVITLVIFVIGGTYAIYYGVRFLLNLVLKREHKNWVTQNYKK
                     HSGVNLSRVNKVVTSGALPVEELSHWEMVAVFGLLLLKTEVPDVIQRAEVFGNQGWRK
                     HFNYEFYDEISNMAKKISGQGYKKNARKLIMFGQRLAELYNEKYWDEQYRKILDEQLS
                     ELSDLEWDFTRGAKP"
     gene            complement(789..1310)
                     /locus_tag="PLT08_18155"
     CDS             complement(789..1310)
                     /locus_tag="PLT08_18155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE06457.1"
                     /translation="MKDSSDINEEPYSIRRARAFFFGLKIFIVIILCVGVLAAFGKIS
                     DLIFWNFTTNASPERITSIEIREVSDSDPRNNQVIIVEDKEAISRFVKALSAIQEYDP
                     HQISGKHNLRILVKLNVIETIEIDCFTIQESNRVIVRSVFISPNFLAASVTTAIFPES
                     DFYNWLISVGVNL"
BASE COUNT          388 a          292 c          289 g          423 t
ORIGIN      
        1 aaactgcttg ggatgtgcgc agaatcccca gcgtcaggtg cacgctttgt tgggcggttt
       61 tgttttagga aggcgcatag cattacggtt tagctcctct agtgaaatcc cattcgagat
      121 cggaaagttc tgaaagttgc tcatcaagaa ttttcctgta ttgttcgtcc cagtatttct
      181 cattgtacaa ctctgctaat cgctgtccaa acattattaa tttcctggca tttttcttgt
      241 acccttgccc agaaattttc tttgccatgt ttgagatttc atcatagaac tcatagttga
      301 agtgctttct ccagccttgg ttgccaaata cttctgctcg ttgaattaca tcgggaactt
      361 cggtcttaag taagagtaaa ccaaaaacag cgaccatttc ccaatgtgat aattcttcaa
      421 caggtaaagc gccgctagtg actaccttgt ttactctgct gaggtttact cctgagtgtt
      481 tcttgtaatt ttgagtcacc caatttttgt gttctcgctt aagcacgaga tttagcagga
      541 acctcacacc ataatatatt gcataagtcc caccaattac gaatataaca agtgtaataa
      601 catccaacat ttgtttacct ttgcttttga aagaaccgcc caacggtttg cgttagcgga
      661 cggcgggcga gaatcaactt cgacgaacgg agaaaactcg aagcgaggaa aatgcttgaa
      721 aacggcgcag aatccccgct gtccgctgca cgctttgttg ggcggcgttt cttcataact
      781 aataattatt ataaatttac tccaacgcta attagccaat tatagaaatc tgattcaggg
      841 aaaatggcgg tggtgacact tgcggcaagg aaattgggag agataaaaac agagcggaca
      901 ataactctat tgctttcctg aattgtaaaa caatcaatct caatagtctc tatgacattc
      961 aattttacca agatacgtag attgtgtttt ccagaaattt gatgagggtc atattcttgg
     1021 attgcggata atgctttaac aaaacgacta atcgcttctt tatcttcaac aataatgact
     1081 tgattatttc tagggtcaga atcactgact tcccgaatct ctatgcttgt aattctttct
     1141 ggtgatgcgt ttgtagtgaa attccaaaag ataaggtcac tgatttttcc gaaagcagcc
     1201 agcacgccaa cgcataaaat tataacgatg aagattttta atccaaaaaa gaaggcgcga
     1261 gcgcggcgga tagaatatgg ctcttcattg atgtcagaag aatctttcat ggtttcactt
     1321 aatatcttgg tttgctagcc agccgcccaa cggtttgcgt tacctgcgct ggggcgggga
     1381 cggcgaagcc gt
//
LOCUS       DAOQJE010000942         1380 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_96010_length_1380_cov_4.037736, whole genome shotgun sequence.
ACCESSION   DAOQJE010000942 DAOQJE010000000
VERSION     DAOQJE010000942.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1380)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1380)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1380
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_96010_length_1380_cov_4.037736"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(105..713)
                     /locus_tag="PLT08_18160"
     CDS             complement(105..713)
                     /locus_tag="PLT08_18160"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HNE06458.1"
                     /translation="MSSNFPDNEDPRITRTRNLIFNAYLELLREKGFPNLTVHDVTER
                     AGINRVTFYSHFADKYALLDFSIRELFRQEIEKHGFNACHYNPDNLRLLIVAVCEFVS
                     NAHANCSSSDSETQFKSLIETQVKKQIQEVLQMWLEQVDSTADLKTVTTAASWSLYGL
                     ILQWNQDKKKKKPSAEQFADQTLPLIMVILGETKQPAVLSVK"
     gene            846..1217
                     /locus_tag="PLT08_18165"
     CDS             846..1217
                     /locus_tag="PLT08_18165"
                     /inference="COORDINATES: protein motif:HMM:NF024953.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HNE06459.1"
                     /translation="MNVALWIVQIFFGLSFSMIGVMKTFMTAKAKETLPWAKDRSDAF
                     VRFVGMSELLGGLGMILPMLTGILPWLTPLAAAGLGIIQILAIFTEHLPKKEYNVLPM
                     NIFLLALAVFIAWGRWGLFGG"
BASE COUNT          363 a          280 c          301 g          436 t
ORIGIN      
        1 aacaacatat tcttacaaac caacgaatgc aaggttgact ttaacagcca agccattatg
       61 gaattaatcc acattttttc tccttagatc tattcttttt gaatttattt aacgctcaac
      121 acggcgggtt gttttgtctc ccctaaaatg accatgatca acgggagtgt ttggtccgca
      181 aattgttccg cagagggctt tttctttttc ttgtcttgat tccattgcag aatcaatcca
      241 tagagcgacc aacttgcggc agtggtcacc gttttcagat ctgcagtcga atcaacctgc
      301 tccagccaca tttgcaacac ttcttgaatt tgcttcttta cctgtgtttc tatgagcgat
      361 ttgaactgcg tctccgaatc agaagatgag cagtttgcat gggcattcga gacaaattca
      421 cagactgcca cgatcaacaa gcgcaaatta tcggggttat agtgacaagc gttgaaacca
      481 tgtttttcaa tctcttgtcg aaataactca cgaatactga aatcgagcaa ggcgtattta
      541 tcagcgaaat gtgaataaaa tgttacacga ttgatgcctg ctctttcagt aacatcatga
      601 actgtgaggt ttgggaatcc cttttctcgg agcaattcaa gataagcatt aaagataaga
      661 ttacgagtgc gcgtaatgcg tgggtcttca ttatctggaa aattagacga cactttctct
      721 cctctgtagt ttaggctaca aaaccagcct tttgatggtt gattttccat atgcgaacgg
      781 atataaacta catatgttgt ttaatagaca aatgtagttt acacctttca attaggagag
      841 tcaagatgaa tgtcgcatta tggattgttc aaatattttt tggcctatct tttagcatga
      901 tcggcgtgat gaaaactttt atgacagcga aagccaaaga aactttgcct tgggcaaaag
      961 atagatcaga tgcttttgtt cgcttcgttg gaatgtcgga attgctcggc ggattgggaa
     1021 tgatcttgcc gatgttgaca ggtattttgc catggttgac accgcttgct gcggcgggct
     1081 tgggtattat tcagattctg gcaattttca ccgagcactt gcctaaaaaa gaatacaacg
     1141 ttttgcctat gaatatcttt ttgttggctc tggcagtctt tatcgcctgg ggtcggtggg
     1201 gattgttcgg aggctaggct ttactgcaca gtatttatac ctgtatctgt ttctccccag
     1261 aaaaaaacgc cccgccctcg aaaaattcgt ggacgaagcg tatccgttag tggcggttaa
     1321 tttagagcag tttgcgtaag atgttcgcag gggcgcggtt tccgcgccct attttatata
//
LOCUS       DAOQJE010000943         1377 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_96423_length_1377_cov_5.869138, whole genome shotgun sequence.
ACCESSION   DAOQJE010000943 DAOQJE010000000
VERSION     DAOQJE010000943.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1377)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1377)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1377
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_96423_length_1377_cov_5.869138"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(29..>1377)
                     /locus_tag="PLT08_18170"
     CDS             complement(29..>1377)
                     /locus_tag="PLT08_18170"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HNE06460.1"
                     /translation="MMHDPQFRERFEREIKMIASLEHPAIVPVYDVGDIDGQPYFVMR
                     FMTGGSLSDLIEKGSIPIDETAKIIEKIAQGLAYAHRKGVIHRDLKPDNILFNDDGEP
                     FISDFGVAKLSESSSNLTGSGVIGTPAYMSPEQAQGNEIDQRSDVYGLGVIVYQMLSG
                     HQPYSADTPMGVVVKHITEPVPEILKVLPNLPEDVDAIIKTAMAKDKTQRYANTIELA
                     KALNTVAFGTPGDITFHTMNGRTRLPSNSKPLSRGQTTGLAVGGVILLLAIVGVFLLR
                     NQLFASSSPTSTTDAASTEVSETSTPVLVVVTATLPEATLASGPASAPACSANVSFPA
                     PIGKETNSFCTQKIPYAYANMADHATFTILDSSTAGAKCRQEAIKNGEKVVSCTGPSF
                     AVIDLEVCEPVELKPEELSQCSADSTYNAANQCCVQQPPQKASCKVIKIQLKGCGG"
BASE COUNT          335 a          320 c          357 g          365 t
ORIGIN      
        1 aatcgggcga ttgctcgccc gattttgttt atccgccgca gcccttaagc tggatcttta
       61 ttactttaca tgatgctttt tggggcggct gttgaacaca gcattgattg gctgcattgt
      121 aggtgctgtc tgctgagcat tgagaaagtt cttcaggttt taattcaacg ggttcacaga
      181 cttcaaggtc gatcactgcg aaactgggac cagtacacga aacgaccttt tcgccgttct
      241 taatcgcttc ctgacgacat ttcgcaccag ctgtgctgga atctaatatt gtaaacgttg
      301 catgatctgc catattggca taggcatacg gaatcttctg tgtacagaaa ctattggttt
      361 ctttcccaat aggtgcaggg aaggaaacat tggcagaaca agcgggtgcc gaggcgggac
      421 cagaagctaa tgtcgcttcg ggcagggtag cggtgactac aaccaagacc ggtgttgaag
      481 tttcactgac ttctgttgag gctgcatctg ttgtagaggt tggcgagctg gatgcaaata
      541 gctgattgcg gagcagaaac actccaacga tggctaaaag cagaatcacg ccgccgactg
      601 ccaagccagt ggtttgcccg cgcgataagg gctttgaatt ggaaggcagg cgtgtacgtc
      661 cattcatggt gtggaaggta atatcgcccg gcgtaccaaa tgccacggta ttcaacgcct
      721 ttgccaattc aatggtgttg gcgtaacgtt gagtcttgtc ttttgccatc gctgttttga
      781 tgatggcatc tacatcttca gggagattgg gaagtacttt aagaatttcg ggcaccggct
      841 cggtgatgtg cttgacgacc acacccatgg gggtatcggc gctgtaaggc tgatgtccgc
      901 tcaacatttg gtagacgatc acgcccaagc catacacatc actgcgttga tcgatttcat
      961 tcccctgagc ctgttcagga ctcatatagg caggagtacc gatcacaccg ctaccggtta
     1021 gattactgct tgactcagat aatttggcaa caccaaagtc ggagatgaac ggctcaccat
     1081 cgtcattaaa gagaatattg tctggcttca gatcgcgatg gataacacct ttgcgatggg
     1141 catatgccaa gccctgagcg atcttttcga tgatctttgc tgtctcatca atcggaatac
     1201 tgcctttttc gatgagatcg gaaagggagc cgcccgtcat gaagcgcatg acaaaataag
     1261 gctgaccatc aatatcgcct acatcataca caggcacaat ggctggatgt tccaatgagg
     1321 cgatcatctt gatctcacgt tcaaagcgtt cgcggaattg agggtcgtgc atcattt
//
LOCUS       DAOQJE010000944         1376 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_96563_length_1376_cov_6.311885, whole genome shotgun sequence.
ACCESSION   DAOQJE010000944 DAOQJE010000000
VERSION     DAOQJE010000944.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1376)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1376)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1376
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_96563_length_1376_cov_6.311885"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(7..492)
                     /locus_tag="PLT08_18175"
     CDS             complement(7..492)
                     /locus_tag="PLT08_18175"
                     /inference="COORDINATES: protein motif:HMM:NF014602.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CarD family transcriptional regulator"
                     /protein_id="HNE06461.1"
                     /translation="MEFQSGDWVVHCAHGLGQITAIETRSFGEQTIPYYQVQIGDLTI
                     WVPTDEHLGKRLRKPASQSDFSNLLNMLTNPPETLPSDRRQRNQYLIELLKDGTAETL
                     CKVIRDLSAIRKHRTWSEYDRDILRRVQKSLIGEWSFIFSINPHDAENELQKLLAKHI
                     A"
     gene            complement(610..885)
                     /locus_tag="PLT08_18180"
     CDS             complement(610..885)
                     /locus_tag="PLT08_18180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE06462.1"
                     /translation="MTKEKIKAFEEIARIFEISNESAKYFLNHVQKSFKVERPPHKLI
                     LEFVEKQNYEFKPTPYDVALAMKESGVWDYDLGSPPPALEDEEDLYQ"
     gene            complement(885..1151)
                     /locus_tag="PLT08_18185"
     CDS             complement(885..1151)
                     /locus_tag="PLT08_18185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE06463.1"
                     /translation="MQTCPICNKEVQPSQRYPNYVCAECADLAVSKDGRKLIFYNQSI
                     SGGYEAKYADTSEEYNSHDCYINGIKCHANEHHFGGIVIIKVES"
     gene            complement(1155..>1376)
                     /locus_tag="PLT08_18190"
     CDS             complement(1155..>1376)
                     /locus_tag="PLT08_18190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE06464.1"
                     /translation="VGAAVAQWNSGRTRLTLCGYSKTPMLAMDGTEADGIQEAARNAY
                     HEANDEWASAEYRMDVAAVLAGRALTALT"
BASE COUNT          321 a          298 c          335 g          422 t
ORIGIN      
        1 ttttccttat gctatatgtt tggctaataa tttttgcagt tcgttttctg cgtcgtgcgg
       61 gttgattgaa aaaataaagc tccactcacc gatcaacgat ttttgtacgc gccgcagaat
      121 atctctgtcg tattcgctcc atgtgcgatg cttgcgaata gcggataggt cgcgaataac
      181 cttgcataag gtttcggcgg taccgtcttt gaggagttca atgagatact ggttgcgttg
      241 acggcggtca ctaggcagtg tctctggtgg attcgttagc atgtttagca gattactaaa
      301 atctgactgg cttgcgggtt tgcgcaagcg tttgcctaaa tgctcatctg tgggaaccca
      361 aatagtgaga tcgccaattt gaacctgata atagggaatg gtttgctccc caaaagaacg
      421 cgtttcaatc gctgtgattt gccccaagcc atgcgcacaa tggacgaccc aatcgcctga
      481 ttgaaattcc atataagtct cctttgtact tgatgggaaa agaaaagcgc cagtccgaca
      541 aaaggactgg cgcttagaca ctgtcaatct gttatatagg gatgattata tcaccgatgc
      601 tagctgcttt tattgataga gatcttcttc gtcttccaat gcaggcggtg gagagccgag
      661 gtcgtaatcc catacgccgc tttctttcat ggctaaggca acatcgtatg gagttggctt
      721 gaattcataa ttttgttttt caacaaattc aaggatcaat ttatggggcg ggcgttcaac
      781 tttaaagctt ttctgaacat ggttgagaaa atactttgcg ctttcgttgg atatctcaaa
      841 aatacgcgcg atttcttcaa aggctttaat tttttctttg gtcattagct ttctactttt
      901 atgatcacaa tgccgccaaa gtgatgttcg ttggcgtgac acttaatccc attaatataa
      961 caatcgtggc tgttatattc ttcgctggta tcagcgtatt tggcttcata tccaccagaa
     1021 attgactggt tgtagaagat tagttttctg ccatcttttg atactgcgag gtcagcacac
     1081 tcggcgcaga cgtaattggg gtaacgttgc gagggttgca cttctttgtt gcagattggg
     1141 caggtttgca tggactaggt aagtgctgtc aatgcacgcc ctgccagcac tgccgcaaca
     1201 tccatgcggt attcggcaga agcccattcg tcgttggctt cgtgataggc gttgcgtgcg
     1261 gcttcttgaa tgccatccgc ttcggtacca tccatggcga gcatgggagt tttgctgtat
     1321 ccacacaggg taaggcgggt tcgccctgaa ttccattgtg cgacggcagc tccaac
//
LOCUS       DAOQJE010000945         1372 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_97175_length_1372_cov_4.599089, whole genome shotgun sequence.
ACCESSION   DAOQJE010000945 DAOQJE010000000
VERSION     DAOQJE010000945.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1372)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1372)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1372
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_97175_length_1372_cov_4.599089"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..592)
                     /locus_tag="PLT08_18195"
     CDS             complement(<1..592)
                     /locus_tag="PLT08_18195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE06465.1"
                     /translation="MDFQQIFDVQQIIIYALGFTITIFGIYLIITGLWGVIKKRPLVV
                     PASHYLGFMFAIFLLGLVLTFVPFLSSWKHRDSFLSTAPFVNLAMIILMIFMLRRQMS
                     GYTVLGIYDDTFREALTSALTKLNLSFQENISKIRLTDLNIDLQATVASWMGTAQIHI
                     KQSHSSRRIKDIANAMNDYFVNTPVKVNYFAFITYLI"
     gene            complement(880..1299)
                     /locus_tag="PLT08_18200"
     CDS             complement(880..1299)
                     /locus_tag="PLT08_18200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE06466.1"
                     /translation="MKFKNIIAVLLIAFILVVCGLSLIYSWDESLFWKNLDIKPNEIP
                     IYPNAQSITQEEPILDGYELYRWNFITNDTPDKVWSFYADKMSNKWGFYDTSSEKFND
                     HSLVVKSCPFYHLSMTDTSIDDKTYKIFIEFGKEPCR"
BASE COUNT          428 a          257 c          289 g          398 t
ORIGIN      
        1 gaatcaagta tgtgataaag gcaaagtaat ttactttgac gggcgtattt acaaagtagt
       61 cattcattgc atttgcaata tccttaatgc gtcgagaaga atgagactgc ttgatatgaa
      121 tttgagcagt tcccatccaa gacgcaacgg tggcttgtaa atctatattt aaatcggtca
      181 accttatttt ggagatgttt tcttgaaacg aaagatttaa ttttgtaaga gcggaagtga
      241 gcgcttctcg aaaggtgtcg tcgtaaatgc caagcactgt atatcctgac atttgacggc
      301 gcaacatgaa tatcatcaag ataatcattg ctaaatttac aaacggcgca gtactgagaa
      361 acgaatctcg atgtttccat gaacttaaga aagggacaaa agttagcaca agcccaagaa
      421 gaaagatagc aaacataaac cccagatagt gacttgctgg cacaactaga gggcgttttt
      481 ttattacccc ccacaagcca gtgatgatta aatatatgcc aaaaatagtt atagtgaatc
      541 cgagtgcata aataattatt tgctgaacat cgaaaatctg ttgaaagtcc ataaggtgaa
      601 ttgttccttt tcgtttatct tttgcggaca gccgcccaac ggtttgcgtt acctgcgtgt
      661 gggcgggcgt ggattctatt tgagagtagg aaaaactcga agccagaaaa atgcttgaaa
      721 atgccgcaga atcccacacg tcaggtgcac gctttgttgg gcggcgtttt gataatcgta
      781 acattgaata ataaaaggaa caacattaag agtctggtaa ttctagaaaa gtaactttgc
      841 tattatggaa tctttacttt tattgcaaac cagtttattt taccgacacg gttcttttcc
      901 aaactcaatg aaaattttgt aggtcttatc gtcaatcgaa gtgtcggtca tgctcaaatg
      961 gtaaaaaggg caggacttaa caacaagact gtggtcatta aatttttctg atgaagtgtc
     1021 ataaaatccc cacttgttac tcatcttgtc tgcgtaaaaa ctccaaactt tatcgggcgt
     1081 gtcgttagta atgaaattcc atctatagag ttcgtatcca tcaagaatag gttcttcttg
     1141 tgtgatgctt tgagcattag gatatatggg aatttcattc ggtttgatgt cgagattttt
     1201 ccagaatagt gattcgtccc acgaataaat taaagataac ccacaaacga ccaagataaa
     1261 agcgataagc aaaaccgcaa tgatattttt gaatttcata gccgcctatc ctttacgcca
     1321 gccgcccaac ggtttgcgtt acctgcgctg gggcggggac ggcgaagccg tc
//
LOCUS       DAOQJE010000946         1372 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_97180_length_1372_cov_4.208808, whole genome shotgun sequence.
ACCESSION   DAOQJE010000946 DAOQJE010000000
VERSION     DAOQJE010000946.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1372)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1372)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1372
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_97180_length_1372_cov_4.208808"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..605)
                     /locus_tag="PLT08_18205"
     CDS             complement(<1..605)
                     /locus_tag="PLT08_18205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE06467.1"
                     /translation="MGTIVKCPQCNEINFGSQLHCVKCQTSLIGIPREQGESPVPEFV
                     SSPPPPPRENYYSKPLEFKEKKNLWAIAGIVIAVICICLALVGTGVGKIMVERAPIES
                     ILDTFMKDMVAKDIESAYALFSPRVQRQIPIDDIQKMIDGNNYILFDGYKNLSVQNLN
                     LTATANTNPDLPQGTVANVNGFIEYSDGFTGNFTAILEKVDG"
     gene            complement(994..1245)
                     /locus_tag="PLT08_18210"
     CDS             complement(994..1245)
                     /locus_tag="PLT08_18210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE06468.1"
                     /translation="MQNGVCPKCNSRVIIKNKPLLTKGDYDGIKYLFLMIVGKRTGWF
                     FRKSTKGNLRAWVCGECGYTEIYTINFKEFLDADHELKT"
BASE COUNT          411 a          285 c          266 g          410 t
ORIGIN      
        1 ccatcaactt tttctagaat tgccgtgaag tttccagtga atccatctga atactcaatg
       61 aagccgttaa catttgcaac agttccttgc ggtaaatcag gatttgtgtt tgcagtagcg
      121 gtaagattta aattttgtac cgataggttt ttatacccat caaaaagaat gtaattattc
      181 ccatcaatca ttttttgtat atcatcaata gggatttgcc tttgaacacg gggggaaaat
      241 aatgcatagg cactttcaat atcttttgct accatgtctt tcatgaaagt gtccaaaatc
      301 gattcgattg gggcgcgttc taccatgatt ttaccaacac cagtgccaac gagagcaagg
      361 caaatacaaa ttacagcaat cacaatgcct gcaattgccc aaagattctt tttttctttg
      421 aactccaaag gttttgaata gtaattttca cgaggaggag gtggtggcga actaacaaac
      481 tcaggaactg gagattctcc ttgttctcgt ggaattccaa tgagacttgt ttggcatttg
      541 acacagtgaa gttgacttcc aaaatttatt tcattgcatt gaggacattt tacgatagtt
      601 cccataaatc tgttccctct tgtaaagtaa gatgctttag tttcccattg tagcggtaac
      661 cgcccaacgg tttgcgttac ttgcgctggg gcggggacgg cgaagccgtc cagtcagaaa
      721 aatgaaaagg cgtagaaaac tgcttgaagt gtgcgcagaa tccccagcgt caggtgcacg
      781 ctttgttggg cactgtatga atgcaagact cgcttgccga aaaaatgacg ctatcgcata
      841 tctgactaaa ttttacacgc aacttttact ctgacaatac aaattcggca ttgttcatgt
      901 taagttcgcc ctgtataaac cgaatgaaaa caacagttcc tcaaaaatac gaagatgatt
      961 taaaaaaata ctacccgaat tattatcttc gttttaagtt ttcaattcat ggtctgcgtc
     1021 aagaaactct ttaaagttaa ttgtgtatat ttcagtgtac ccacattcac cgcaaaccca
     1081 cgcccgtaaa ttacccttag ttgatttgcg aaaaaaccat cctgtacgct ttccaacaat
     1141 catcaaaaac aaatatttaa tgccatcata atcaccttta gttaacaagg gtttgttttt
     1201 aatgattacg cgagagttac atttagggca aacgccattt tgcattaaat cttccttgtc
     1261 tattattgca aattgtattt atgctgaaaa ccaagcgccc aacggtttgc gttacccgcg
     1321 ctggggcggg gacggcgaag ccgtccaacc agaaaaaggc taaggcgtag aa
//
LOCUS       DAOQJE010000947         1370 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_97451_length_1370_cov_6.717110, whole genome shotgun sequence.
ACCESSION   DAOQJE010000947 DAOQJE010000000
VERSION     DAOQJE010000947.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1370)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1370)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1370
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_97451_length_1370_cov_6.717110"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..381
                     /locus_tag="PLT08_18215"
     CDS             <1..381
                     /locus_tag="PLT08_18215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HNE06469.1"
                     /translation="QKMLKDAEETCAQLGIPYRVKQLCTGDIGFGATMTYDLELWAPG
                     CNEWLEVSSVSNDTDFQARRANIKFRPSDGGKVKFPHTLNGSGLGLPRALIAVIENYQ
                     QADGSIVVPEVLRPWMGGIDVIKP"
     gene            522..1049
                     /locus_tag="PLT08_18220"
     CDS             522..1049
                     /locus_tag="PLT08_18220"
                     /inference="COORDINATES: protein motif:HMM:NF016213.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF456 family protein"
                     /protein_id="HNE06470.1"
                     /translation="MNPADWELFGQVLLEVVTFTVLVFGLLGLLMPVFPGLVVMWIAT
                     LIYAIVQAGLGNMTWVGWTLFVLITLLMIGGNVVDNIIIAKHMRDKDVPWSSILWSFA
                     AGILASLFFTPIIGILASPVGLFLAEWYRLKDRPAAFSSTKTWLTGWGWSVAARIGIG
                     IIMIAFWGLWAWNTG"
     gene            1075..>1370
                     /locus_tag="PLT08_18225"
     CDS             1075..>1370
                     /locus_tag="PLT08_18225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE06471.1"
                     /translation="MLSEPGQLLLNGLTLTALILSLLSLFFPLFPGLIVMWLALLPYI
                     GIQALTGNLHGSSWLLFAIITVLMLGGSVADIFIIDGHLREKQVPGSSVFWSFMA"
BASE COUNT          280 a          331 c          376 g          383 t
ORIGIN      
        1 cagaagatgt tgaaagatgc cgaagagact tgtgcgcagt tgggcattcc gtaccgtgtc
       61 aagcaactct gcaccggcga catcggcttc ggtgcgacca tgacttacga cctcgaattg
      121 tgggcgcccg gctgtaacga atggctcgaa gtctcatcgg tttcaaacga tacagacttt
      181 caagcccgcc gcgcgaacat caaattccgc ccaagcgatg gcggcaaggt taaattcccg
      241 cacacactta acggctcagg tttgggcttg ccgcgtgcac ttatcgccgt gatagagaat
      301 taccagcaag ccgacggttc catcgtggtg cccgaggtcc tgcgtccgtg gatgggcggc
      361 atcgacgtca tcaagccgtg acggtaagga acgaaacggg ttaatatccg tacatatata
      421 aacagtaggg gcgacccgtc cattgggagc gaatatctca atgaaatgtg cagggtcgcc
      481 cttactattt ccggtgcctt tcttatcaaa ggagaaacga tatgaatccc gccgattggg
      541 aattgttcgg gcaagtcttg ttggaagtgg ttacatttac tgttttagtg tttggtttgt
      601 taggtttgct catgcctgtg ttccctgggt tggtcgtgat gtggatcgcc actctcatct
      661 atgccatcgt ccaggcaggg cttgggaata tgacctgggt gggctggacc ctgttcgtgc
      721 tcattacctt attgatgatc ggtggcaatg tagtagataa catcatcatc gccaaacaca
      781 tgcgtgataa agacgtgcca tggagttcca tcctttggtc tttcgctgcc gggattcttg
      841 ccagcttgtt ctttactccc attattggga tccttgcctc accagttggg ctttttctag
      901 cggaatggta ccgcctcaaa gaccgacctg cagccttttc tagcaccaaa acttggctca
      961 ccggctgggg ctggtctgtc gccgcacgga tcgggattgg catcatcatg atcgctttct
     1021 gggggctgtg ggcttggaac acaggttaaa taggagagtc ctatcatttc gtttgtgctg
     1081 agtgagcccg gtcaactgct attgaatgga ctgaccctga ccgcactcat cctcagtttg
     1141 ctaagtttat tttttccgct ctttcccggt ctgatcgtga tgtggttggc tttgctgccg
     1201 tatatcggca ttcaagcgtt gacggggaac ttgcacgggt caagttggtt gctctttgcc
     1261 attatcacgg tcttgatgtt aggcggcagt gtggcagata ttttcattat tgatggtcat
     1321 ctgcgcgaga aacaagtgcc gggcagttcg gtattctggt cgtttatggc
//
LOCUS       DAOQJE010000948         1370 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_97461_length_1370_cov_4.611407, whole genome shotgun sequence.
ACCESSION   DAOQJE010000948 DAOQJE010000000
VERSION     DAOQJE010000948.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1370)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1370)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1370
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_97461_length_1370_cov_4.611407"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            116..313
                     /locus_tag="PLT08_18230"
     CDS             116..313
                     /locus_tag="PLT08_18230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE06472.1"
                     /translation="MNIKKLVVEFATVFAVTLVTAALVTFLWNLIGHGQSVVDWESSF
                     RFATIFGIILTWVKLRETKEK"
     gene            322..993
                     /locus_tag="PLT08_18235"
     CDS             322..993
                     /locus_tag="PLT08_18235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE06473.1"
                     /translation="MENKLLFIGALGAFFLAAAATLVSDMHGIGDWFVSFLQSRKYAL
                     NPAPIYGAPYTIAGYFGEPLLIISIAMLTAGIYGSWLKYHKSIALIAMIIGILYILKR
                     VFWSYVTINFANHIQAHPAITDYTALTQAGFLKGLGALFGGLIGGFGFSTALTILFFS
                     LRNPYGIAGGLVVLAAMLIGMPAKIYFINHVSAAVLTTFIISMVIKNFGIVFMGVGLL
                     TESLK"
BASE COUNT          349 a          344 c          325 g          352 t
ORIGIN      
        1 cgggtctacc cgttcgagca gatcgtcgag gcgcatcgct atgtggaaca aggacataaa
       61 aaaggcaacg tcgccatttc gatgggactg tcccatcaat aataagtaga gaaaaatgaa
      121 catcaagaaa ctggtcgtcg aatttgcaac cgtattcgca gtaaccctcg tcactgccgc
      181 gcttgtaact ttcctctgga acctcattgg gcatgggcag agcgtcgtgg actgggaatc
      241 cagtttccgt tttgcgacga ttttcgggat catcctgaca tgggtgaagt tacgggaaac
      301 caaagaaaaa tagaattttc catggaaaac aaactgcttt tcatcggcgc gctgggagcg
      361 ttctttctgg cagccgccgc cacgttggta tcggatatgc acggaattgg ggattggttc
      421 gtctctttcc tccaatcccg aaagtacgct ttgaacccag cacctattta tggtgccccg
      481 tacacaatcg ctggttattt cggcgaacct ttactcatca tctccatcgc catgctgacc
      541 gcgggaatct acggaagttg gctgaaatac cacaaatcca ttgcgctgat tgccatgatt
      601 attggcatcc tgtatatcct gaaaagggtc ttttggagtt acgtcaccat caacttcgcc
      661 aatcatatcc aggcgcatcc agccatcacc gattacaccg cgctgactca agcggggttt
      721 ctgaaaggac ttggcgcgct cttcggcgga ttgatcggcg gcttcggatt ctccaccgcc
      781 ctgacaatcc ttttcttcag cctccgcaac ccctatggca tcgcaggcgg gctggtcgtc
      841 cttgccgcca tgttgattgg catgcccgca aaaatatatt tcataaatca cgtttctgcc
      901 gcggtgttga ccacctttat catcagcatg gtcatcaaga atttcgggat tgtcttcatg
      961 ggggtgggat tattgacaga gtcccttaag tgaaagatgt tgattatgta agcagatgct
     1021 tgcccaaatt accctgctgc caagttgact cccaatcagc cttttcacag agagagataa
     1081 tgtatcgaag aagacgtaaa tgcccacctc acaaccgcaa tcggatctgt ccagcgcggc
     1141 atatatctgg aatacgggta taaatttaga aaacgaacgt aggcggcaac gtaggcggcg
     1201 gagaaaaaat ctccgccgtt tttatttcaa atgcttttag tcgtactatc aatttgaatt
     1261 agatattggg ttatcgccga cgcgttgcac ggagatcaag gtgacaccca gatcgcgaat
     1321 acgattgaac aagccatgta acgcagactg gtcaaattct ccacggatta
//
LOCUS       DAOQJE010000949         1370 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_97466_length_1370_cov_3.834981, whole genome shotgun sequence.
ACCESSION   DAOQJE010000949 DAOQJE010000000
VERSION     DAOQJE010000949.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1370)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1370)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1370
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_97466_length_1370_cov_3.834981"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1184
                     /locus_tag="PLT08_18240"
     CDS             <1..1184
                     /locus_tag="PLT08_18240"
                     /inference="COORDINATES: protein motif:HMM:TIGR01525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042625 - ATPase-coupled ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HNE06474.1"
                     /translation="KYAIFVLAAAVLLIFVPWLLLGQAFQPTFYRAMTWLVVASPCAL
                     VISTPASILSAIANGARNGVLFKGGVHLEKTATLKVLAFDKTGTLTSGKPTLSELHTF
                     GDFKEDEMLRLVAALESRSEHPLAKAIVQAAHSRNLSLPPSMDFRAIPGQGVEGTVDS
                     HHLWIGNERMFVERDVNIPAAILSKAGDMQSEGQTAMFVYEAKVQKFLGLLGVADTLR
                     EDAVEMIKALKTAGIEHVVMLTGDNTKVAAKIAARAGVDEFHADLLPQDKVTVLKSLQ
                     KKYGPVAMVGDGVNDAPSLATADIGIAMGGAGTDVAIETADVVLMSDDLRKIPFSIGL
                     ARQARKVVWQNLTFALAVIVLLIASAFGANLPLPLGVVGHEGSTVIVVLNGLRLLGYR
                     A"
BASE COUNT          324 a          323 c          366 g          357 t
ORIGIN      
        1 aaaagtatgc gatcttcgtg cttgcggcgg cggtcttatt gatttttgtg ccgtggcttt
       61 tgttggggca ggcgtttcaa ccaaccttct atcgcgccat gacctggcta gtggttgctt
      121 caccgtgtgc cttggtcatc tctacacctg ccagtattct ttcagccatc gccaatggag
      181 cgcgcaatgg tgtgctcttc aaaggtggtg tgcatcttga aaagaccgcc accctcaaag
      241 tccttgcctt cgataaaacc ggcactctca ccagtggtaa accaacactg agtgaacttc
      301 atacctttgg tgattttaaa gaagatgaga tgctgcgact cgtggcggct ttggagtctc
      361 gctccgagca tccgcttgcc aaggcgattg tacaggcggc tcattctcgc aacttgagcc
      421 tgcctccgtc catggacttt cgtgccatcc ccgggcaggg cgtggaaggg accgttgact
      481 cccatcattt gtggatcggt aatgagcgca tgtttgtcga gcgcgatgtc aatattccag
      541 cagcgatatt aagcaaagct ggcgatatgc aaagcgaagg tcaaaccgca atgtttgtgt
      601 atgaagcaaa ggtgcagaaa ttccttggct tgctcggggt agcagacaca cttcgtgaag
      661 atgcagtaga aatgattaaa gccttgaaaa cggctggcat tgagcatgtt gttatgttga
      721 ctggtgacaa tacaaaagtg gctgccaaga tcgccgcccg cgccggagtg gatgaattcc
      781 atgccgacct gctgccacaa gataaagtga cggttttgaa atccctccaa aagaaatacg
      841 gtcctgtcgc tatggtaggc gatggtgtga acgacgctcc atcccttgca actgccgata
      901 ttggcatcgc catgggcggc gctggaaccg atgttgccat cgaaaccgca gacgtggtct
      961 tgatgtctga cgatttgcgc aagattccct tctctattgg gttggcacgc caagcacgca
     1021 aagttgtttg gcagaacctg acctttgcct tggctgtgat cgtgcttcta attgccagtg
     1081 ccttcggcgc aaatctaccc cttccgctcg gggtggtggg gcatgaaggc agtaccgtga
     1141 tagtagtgtt gaatggtttg agattgttag ggtatcgggc gtaaattttt tgattgaaga
     1201 aaataaataa aaagcggatg aatgtcaaaa tacattcatc cgctttttgt tttgttatat
     1261 gagctttcta cggcaaagcc ttaatccctg ggtttcgggc aagcaccaag cgcgagagaa
     1321 ccagcgaaag aatcccgaaa aggatcatta cgaaggtcag accgaatttt
//
LOCUS       DAOQJE010000950         1362 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_98667_length_1362_cov_5.189748, whole genome shotgun sequence.
ACCESSION   DAOQJE010000950 DAOQJE010000000
VERSION     DAOQJE010000950.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1362)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1362)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1362
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_98667_length_1362_cov_5.189748"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..796
                     /locus_tag="PLT08_18245"
     CDS             <1..796
                     /locus_tag="PLT08_18245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE06475.1"
                     /translation="GRLRRPRPSAGNANRWAFPSKRNMKTQLSLAIILISVLVSCSSA
                     SNTISPTQIPTETAVSTIAPVPYTPEPSITVTPLVVEQSYSSEIYPASLFTKKLSEIV
                     LSPDEIPALDPNYPYLIQPQAQDLTNGISNCNNDCQSIVWVSQKAEGFGDRQVSIILI
                     RMDTDSQAAESVVNSWNNFSQVESGTGFVEGTLIQSDLLPNNSKVGIKYKEPDVEVMF
                     TASRGPIYMMFVYSFKASGDLALDFGSIQHLAILQIKKLEAAGYPK"
     gene            975..>1362
                     /locus_tag="PLT08_18250"
     CDS             975..>1362
                     /locus_tag="PLT08_18250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE06476.1"
                     /translation="MEINFLIPLLIFLSIIAVLGIFLYLYLRQKQNRLIPKDIYDESQ
                     SEYHTRKNRLPYNIATSSSMSSRLLAFYGTSVGFVTLFLLYVAISVSASIWVIIGIVC
                     LSPVTSPFFLLIFPIGLDIFIVPDSNG"
BASE COUNT          385 a          287 c          263 g          427 t
ORIGIN      
        1 tggacggctt cgccgtcccc gccccagcgc aggtaacgca aaccgttggg cgttcccttc
       61 caaacgaaat atgaagactc aactctctct tgcaatcatc ctaatcagcg ttctagtttc
      121 ctgttcatct gcaagtaata ctatttcccc aacgcagatt ccaactgaaa cggctgtttc
      181 aacaattgct cctgtacctt atacgcccga accttcgatt actgtgactc cactagtcgt
      241 tgaacaatcg tattcatcag aaatttaccc cgcctcgctt tttacaaaaa aactttcgga
      301 aatcgtgtta agtcctgatg aaattcccgc acttgatcca aattatccgt acttaataca
      361 gccacaagct caagatttga caaatggtat ttcaaattgc aataacgatt gccaatctat
      421 tgtttgggta agtcaaaaag cagaaggttt tggtgatcga caagtttcta taatattgat
      481 tagaatggat acagattcac aggcggcaga aagtgttgtt aatagctgga ataatttttc
      541 gcaggtcgaa tctggcacag gttttgttga aggcacttta attcagtcag atttgttgcc
      601 taataatagt aaagtaggta tcaaatacaa agaacctgat gtagaagtta tgttcactgc
      661 ttcgagaggt ccaatatata tgatgtttgt ttattctttt aaagcaagcg gagatttggc
      721 gttagatttt ggaagcatac aacatttggc tatcttacag ataaaaaaat tagaggcggc
      781 aggttatcca aaataaaaac gcccaacaaa gcgtgcacct gacgctgggg agtctgcgca
      841 caccccaagc agtttcctac gccttttcat ttttctggct ggacggcttc gccgtccctg
      901 ccccagcgca ggtaacgcat accgttgggc tataagtcaa ttgcaaatat gagttgtgac
      961 aaataggaga tggtttggaa ataaattttt taataccact gctgattttc ctgtcaatca
     1021 ttgccgttct tgggattttt ttatacttgt atcttcgtca aaaacaaaac aggttaattc
     1081 ctaaagatat ttacgatgaa tcgcaaagtg aatatcatac gagaaagaat cgcttaccat
     1141 acaacatagc aacgtcttct tcgatgagct ctcgccttct cgcattttac ggcacttccg
     1201 ttggctttgt tacacttttt ttgttgtatg ttgccattag cgttagtgct agtatttggg
     1261 taataattgg gattgtttgt ttatcaccag taaccagtcc atttttcctg ctaatatttc
     1321 caattggact agatatattt atagtccctg attctaacgg gg
//
LOCUS       DAOQJE010000951         1358 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_99258_length_1358_cov_5.694551, whole genome shotgun sequence.
ACCESSION   DAOQJE010000951 DAOQJE010000000
VERSION     DAOQJE010000951.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1358)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1358)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1358
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_99258_length_1358_cov_5.694551"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(135..641)
                     /locus_tag="PLT08_18255"
     CDS             complement(135..641)
                     /locus_tag="PLT08_18255"
                     /inference="COORDINATES: protein motif:HMM:NF039330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6036 family nucleotidyltransferase"
                     /protein_id="HNE06477.1"
                     /translation="MEIQDLLNKLGGQIKTPIRLYLIGGSALALLGSPRQTIDIDFIG
                     NDILPTSEHEALLKTAKELDILLEPVPLEQFIPLPENSDKRSIRIGKFNNLEIYVADP
                     YSIALGKLDRGFDTDIEDIIFLLQKHYIEIEKLNLITKTALSQANKYDLNATEILDHL
                     QVVRERLK"
     gene            complement(643..1161)
                     /locus_tag="PLT08_18260"
     CDS             complement(643..1161)
                     /locus_tag="PLT08_18260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE06478.1"
                     /translation="MNRHRKATSEQLVNALHTLGVDFIMGGSNENASMSQSPAQMIAA
                     LAKHNEARLRLSLIPLFLEHPEFSKQAQETAKSLPEKARLFLQCYYSASVWLQKIHHT
                     KLSKLTGKKALLPDLFSFELGIPVTENPETNLRLLAEQHQKLSGEKVNWLGTYKHAAQ
                     VFINGLELKHRG"
BASE COUNT          303 a          268 c          340 g          447 t
ORIGIN      
        1 tggtttgaga gtgacctggg ttcggggagt tcgttcttct ttacgattcc gctgtgaaga
       61 gtagagaata gtgagtagtg agtagaaaaa atccctgctt gtaagcgggg attttttgtt
      121 ttacctaaaa tgatttactt caatctttca cgaaccacct gaaggtgatc cagaatctcc
      181 gtggcattaa gatcatattt gtttgcctgt gaaagggcgg tttttgtaat caggttcaac
      241 ttttcaattt cgatgtagtg tttttgaagc agaaagatga tatcttcaat gtctgtgtcg
      301 aagccccgat ctaatttacc aagcgcgata ctgtaaggat ctgctacata aatttcgagg
      361 ttgttgaatt ttccaattcg aatacttcgt ttgtcgctat tttcaggcag gggaataaat
      421 tgttcaagag gcacaggctc tagaagaata tccagttctt tggctgtttt tagcaatgct
      481 tcgtgttctg aggttggcaa aatatcgttg ccgataaaat ctatatctat ggtttgtcgc
      541 ggacttccca ataacgccag cgcactccct ccaatcagat atagacggat tggagtcttt
      601 atttgtccgc ccagtttatt tagaaggtct tgaatttcca tttcacccac ggtgtttcaa
      661 ttccaatccg ttgataaata cctgtgcagc atgtttgtat gtcccaagcc agttgacctt
      721 ttcgccgctc agtttctgat gctgttctgc cagcaggcgc agattggtct ctgggttctc
      781 ggtgactggt atccctaatt cgaatgagaa tagatcgggg agcagggctt tctttccagt
      841 tagttttgat aattttgtgt gatggatttt ttgcagccag acagaggcgc tgtaatagca
      901 ttgtagaaag aggcgcgctt tttcaggcag actctttgct gtttcctgcg cctgttttga
      961 gaactctggg tgttcaagaa acagtgggat cagagacagg cgcagacggg cttcattatg
     1021 ctttgccagt gcggcgatca tttgtgcagg tgattgtgac atcgacgcat tttcatttga
     1081 cccgcccatg ataaagtcca cccctagcgt gtgcaaagcg ttgacaagtt gctcgctggt
     1141 ggcttttctg tgcctgttca taaaataatt ataacctgcc aattaccttg tttattcgtc
     1201 tgctggcagc gcttttagcg gcttaccatg attttcttca gggtggggat gagcgtttgc
     1261 atgtctgtgg ttgggacatt ggcaagttct tcggggagcg gggcatcatc cagccgcagg
     1321 gtgaggattt ggaattcttc cttggaggtt gtttttgt
//
LOCUS       DAOQJE010000952         1356 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_99569_length_1356_cov_3.705611, whole genome shotgun sequence.
ACCESSION   DAOQJE010000952 DAOQJE010000000
VERSION     DAOQJE010000952.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1356)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1356)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1356
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_99569_length_1356_cov_3.705611"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..694
                     /locus_tag="PLT08_18265"
     CDS             <1..694
                     /locus_tag="PLT08_18265"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HNE06479.1"
                     /translation="FATKAWNYSKKRGSMIHILIAEDHLMVRAGIRALLEKVGDMVIL
                     GEASNGQEAIEMTESLNPDVLIMDIMMPRLNGIQAAEQLRSRKNRVNILLLSMYADEG
                     LVYQALQSGANGYVLKSSASDELVQAVREIANGKKFLSGPISEIVMENAFKPRPANEV
                     DPLASLSPREKEIMQLIAEEHTSVEIAQFLSISEKTVEKYRANLMEKLHVRNLAGLVR
                     LAVKYRLVDRDS"
     gene            818..>1356
                     /locus_tag="PLT08_18270"
     CDS             818..>1356
                     /locus_tag="PLT08_18270"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNE06480.1"
                     /translation="MKPTILIVDDEPSGQQVVESILQDQGYVMEFASTGKEAIKKAGE
                     LKPDLILLDIMLPEIDGIQVCQTLRKTTELAKIPIVMLTALSDRDIRIASLDAGADDF
                     ISKPFDRAELRARVRSITRLNTYRLLYERNMMFSWISDQSNDGYLLIRNGDEVIYANP
                     RARFYLGLELDSNIPAGTS"
BASE COUNT          383 a          260 c          339 g          374 t
ORIGIN      
        1 ctttgccact aaagcctgga actacagtaa aaagagaggg agcatgattc atattctgat
       61 tgcggaagac catttgatgg tacgggcggg gattcgtgca ttgttagaga aagttggtga
      121 tatggttatt cttggtgaag cttcaaacgg acaagaagcc atagaaatga ccgagtctct
      181 taacccagat gtcctaatca tggatatcat gatgccgcgt ctaaacggca tccaagcggc
      241 agaacaactc cggagcagaa agaaccgcgt taatatcctg ttgttatcca tgtatgcaga
      301 tgagggcttg gtatatcaag ccttacaaag tggagcaaat ggatatgtgc tgaaatcttc
      361 tgccagtgac gaactggtac aggcagtgcg cgaaatcgca aatggtaaaa agttcttgag
      421 cggtcccatt tctgagattg tcatggagaa tgccttcaag ccgcgccctg caaacgaggt
      481 agacccgctg gctagtttat ctcctcgtga gaaggagatc atgcaattga tcgcagaaga
      541 gcatacaagc gtggagattg cacaattctt gtcgattagc gaaaaaacag tggaaaaata
      601 tcgagcaaat ttgatggaaa agttgcatgt acgaaatctt gcagggctgg ttcgccttgc
      661 ggtcaaatat cgcctcgttg atagggattc ttagcgcttt gcaatactgg aatccccttc
      721 acaatagggg ttttcctgta tcctcaaaca ggacaagttg aaataccctt ggtcaggtta
      781 gtagtatatg gatccatatt tttaaggaca gatattaatg aaacctacca ttcttatagt
      841 ggatgatgaa ccctctgggc aacaggtggt tgaatctata ttgcaagatc aggggtatgt
      901 aatggaattt gcttccacag gaaaggaagc catcaaaaaa gctggggagt taaagccaga
      961 tctgatcctt ttagatatta tgttgccgga gattgatggc attcaagttt gccagacgtt
     1021 gcgtaaaact actgaacttg ccaagatccc gatcgtgatg ttgactgctt taagcgaccg
     1081 agatattcgc attgctagct tggatgccgg ggcagatgat tttatttcca agcctttcga
     1141 cagggcagag ttacgtgctc gcgtgcgttc gatcacacgc ctaaatactt atcgcctttt
     1201 gtacgagaga aatatgatgt tctcttggat tagtgatcaa tcgaatgatg ggtatttatt
     1261 gatccgaaat ggagatgagg ttatttatgc caacccaaga gcccgttttt atcttggttt
     1321 ggagttggat agcaatatcc ctgcgggtac ttcttt
//
LOCUS       DAOQJE010000953         1351 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_100427_length_1351_cov_1.947531, whole genome shotgun
            sequence.
ACCESSION   DAOQJE010000953 DAOQJE010000000
VERSION     DAOQJE010000953.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1351)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1351)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1351
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_100427_length_1351_cov_1.947531"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(106..519)
                     /locus_tag="PLT08_18275"
     CDS             complement(106..519)
                     /locus_tag="PLT08_18275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE06481.1"
                     /translation="MPSKKTTTTTKKMSATVQVAIITTIGTVIVALITGFITLAQKND
                     NPIVSASSTPNPTATPIPTITTSPTQAPIYSLKSGTLDIPPSSSLRSFFGSGIIKKNF
                     MAKVIFYVPYSANKGVWDVGFTFGDGVRVVVVSSG"
     gene            complement(706..1245)
                     /locus_tag="PLT08_18280"
     CDS             complement(706..1245)
                     /locus_tag="PLT08_18280"
                     /inference="COORDINATES: protein motif:HMM:NF018684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1311 domain-containing protein"
                     /protein_id="HNE06482.1"
                     /translation="MKLHKINLWAIFLLLVGCSNFATSENISTQSPVAPSNLPASTEE
                     IQEGLPSPSATNEFTQSPCEQTATTQIELNECARERAQLSHTKLVSLIDELASRLDET
                     RQERLVEIETTWEKMIIEHCEWQASFFEGGSVRPMWLSECLNQQYLDRIEALRLNLCE
                     GNGMTGECEESLKYKSIVP"
BASE COUNT          390 a          260 c          287 g          414 t
ORIGIN      
        1 actatcttcg taaactgtta gtgataatga gttttgctct ccttcttgga gtgctaaaga
       61 tgcaggaaga tttattaaac tcaggctagg tttattaaat ccccattatc ctgaagatac
      121 aacaacaact ctgacgccgt ctccaaatgt aaagcctaca tcccaaactc ctttatttgc
      181 tgaataaggg acataaaaaa ttaccttagc cataaaattt ttctttataa ttccagaacc
      241 aaagaaacta cgaagtgacg aagatggggg aatatccagt gtgccacttt tcaaggaata
      301 gatgggcgct tgtgttggtg atgttgtaat tgtaggaatg ggagttgccg ttgggttagg
      361 agtacttgac gctgaaacaa tagggttatc gtttttctga gccaaagtaa taaagccagt
      421 tattaaagca actatgactg ttccaattgt ggtaattatc gccacctgaa cagttgcgct
      481 cattttcttt gttgttgtcg ttgttttttt tgatggcata acattaaacc cttttgatac
      541 taagatttag tgaggtacat gaccaaacgc ccaacggttt gcgttacctg cgctggggtg
      601 gggacggcga agccgtccaa ccagaaaaaa gataaggcgt agaaaaatgc ttgagattgc
      661 cgcagaatcc ccagcgtcag gtgcacgctt tgttgggttg cgtttttacg ggacaatact
      721 tttgtatttc aaagattctt cacattcacc cgtcatccca tttccttcac agagatttaa
      781 tcgcaatgct tcgattcggt caagatattg ttggtttaga cattcagata accacatagg
      841 tcgaacagaa ccgccttcaa agaaactagc ctgccattcg caatgttcaa taatcatttt
      901 ttcccaagta gtttcaattt caacgagtct ttcttgtcgt gtttcgtcta atcgggaagc
      961 aagttcatca atcaaagaaa ccaactttgt gtgagataac tgggctcttt cccttgcaca
     1021 ttcatttaat tcaatttgtg ttgtagcagt ttgttcacat gggctttgag taaattcatt
     1081 tgttgcacta ggcgaaggca aaccttcctg tatttcttcg gttgaggctg gtaaattact
     1141 gggagcaact ggagattggg tagatatatt ttcggaagta gcaaaattgc tacaaccaac
     1201 taataacaag aaaatagccc acaggtttat tttatgtaat ttcataatat tttattttgc
     1261 aaggaagcaa cccaacggtt tgcgttaccc gcaagtgggc gggacaagac tcggcttgag
     1321 gcgagtataa acccgaaatg aggaaaaggc t
//
LOCUS       DAOQJE010000954         1348 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_100778_length_1348_cov_6.071926, whole genome shotgun
            sequence.
ACCESSION   DAOQJE010000954 DAOQJE010000000
VERSION     DAOQJE010000954.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1348)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1348)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1348
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_100778_length_1348_cov_6.071926"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..422
                     /locus_tag="PLT08_18285"
     CDS             <1..422
                     /locus_tag="PLT08_18285"
                     /inference="COORDINATES: protein motif:HMM:NF024584.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SPASM domain-containing protein"
                     /protein_id="HNE06483.1"
                     /translation="KKDIAKFNEEIAPRIAERALELGLIVSDEDAFPFGRDESEVRLG
                     RAGRYAFGFYEKHPCYAPWSHTLIDFNGNVYVCCMTREKIPTLGNIIHQSFREIWEGA
                     AYRQVRLKMHPPALKPCARCDDFIDQNKSIWETIGPY"
     gene            complement(419..1027)
                     /locus_tag="PLT08_18290"
     CDS             complement(419..1027)
                     /locus_tag="PLT08_18290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE06484.1"
                     /translation="MNVLLLHGQGRTTNAMRLLGFRLGRLGHPVQYFRYYTRSETFEG
                     IVNRFVQWVNTVNDGQPYAIVGHSMGGLIARAGLPLLKNLPVHLFLLASPNRPPKLAP
                     YVQNNFLYRYLTTDCGQLVLNSNFYQKLPLPNIPATVIAGSAGPRVGWLPYGFEPNDG
                     ALSVQDASMGDDYELIQVPSIHTFIMNSKLTLHYIEQILNQS"
     gene            complement(1033..1314)
                     /locus_tag="PLT08_18295"
     CDS             complement(1033..1314)
                     /locus_tag="PLT08_18295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE06485.1"
                     /translation="MAKCEMVVGYLVPHRCDNPALGTCVKCGRGFCDEHTNATKEGRV
                     CLACQQGLEMPVALPIAASTFTPADMVLFGSTSSFDDNDDNSDMFSDLS"
BASE COUNT          380 a          333 c          317 g          318 t
ORIGIN      
        1 gcaagaagga tatcgccaaa ttcaatgaag agatcgcgcc gcgcatcgca gaacgtgcgc
       61 ttgaattagg tctgatcgtt tcggatgaag atgcttttcc ctttgggcga gacgagtccg
      121 aggtccggtt gggacgcgct ggacggtatg catttggctt ctatgaaaag catccctgct
      181 atgcaccatg gtcacacaca ctgatcgatt tcaatggcaa tgtctatgtg tgttgtatga
      241 cacgcgaaaa aatccctaca ttgggaaaca tcatccatca atcatttaga gaaatttggg
      301 aaggcgccgc ctatcgccag gtgcgcttga aaatgcatcc gcctgcgctg aaaccttgcg
      361 ctcgttgcga tgattttatt gaccagaata aaagtatctg ggaaacgatc ggtccttact
      421 aactctggtt gagaatctgc tcaatatagt gaagtgtcaa ctttgagttc ataataaagg
      481 tatgaatgct cggcacttgg atcaactcat aatcatcacc cattgaagca tcttgcacgc
      541 tcaatgcccc atcattgggc tcaaagccat aaggcaacca accgacacgt ggtccggcag
      601 agccggcgat gaccgttgca gggatattcg gtaatggcaa tttttgatag aagttcgagt
      661 ttagaactaa ttgtccgcaa tctgtagtga gatacctgta gagaaaatta ttttgaacat
      721 aaggtgccaa cttaggcgga cggttcggcg aagccagtaa aaacaaatga actggcagat
      781 tcttgagcaa gggcaaccca gctcgggcga tcaacccgcc catcgaatgc cccacgatgg
      841 catagggttg cccatcattc acagtgttga cccactgcac aaaacggttt acgatgcctt
      901 caaatgtttc gctacgggtg taatatcgaa agtattgcac aggatgccca agcctgccaa
      961 gccgaaagcc aagcagccgc atggcattgg tcgtccgccc ttgcccgtga agtaaaagaa
     1021 cgttcacaaa cgttagctca agtctgagaa catgtcgctg ttatcatcat tatcgtcgaa
     1081 ggaactggtg gagccaaaca ataccatatc cgccggagta aaggtcgaag cggcaatcgg
     1141 taaagcaacc ggcatctcaa gaccttgctg gcaagcaaga caaacacgcc cttcttttgt
     1201 tgcgttcgta tgctcgtcac aaaacccgcg tccacactta acgcacgtcc ccaatgctgg
     1261 gttatcacaa cgatgcggaa ccaaataacc aacaaccatt tcacactttg ccattgagac
     1321 acctcatgaa aattgctttg cttagcga
//
LOCUS       DAOQJE010000955         1346 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_101089_length_1346_cov_6.343145, whole genome shotgun
            sequence.
ACCESSION   DAOQJE010000955 DAOQJE010000000
VERSION     DAOQJE010000955.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1346)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1346)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1346
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_101089_length_1346_cov_6.343145"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..956
                     /locus_tag="PLT08_18300"
     CDS             <1..956
                     /locus_tag="PLT08_18300"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase family A protein"
                     /protein_id="HNE06486.1"
                     /translation="VLKMRKGQNPAKFVKDVARPERVTVALLNYIPFLSGFYAETLDV
                     LKVSLESMRKDAGLPFDLMVFDNGSCEEVREFLIGEKEAGRIQYLILSEKNMGKGGAW
                     NVMLAGAPGEIIAYTDSDVLFSPNWLSRSVELLETFPNVGMVTARPYRTSPELYSSTL
                     EWAKKNATLDEGQFIPWETFLEFNLSLGQTEEENVKVYAETSDWRIQYKGVTALAGAS
                     HWQFTAYKSTLQQFLPFDMDKPMGQVRQLDKRMNDAGLLRLMVSDPLAMNMSNTLGYL
                     RGELKEERRKKVGVGKRVLEFPPIKKMLLAVYNKIFSWYYS"
     gene            991..>1346
                     /locus_tag="PLT08_18305"
     CDS             991..>1346
                     /locus_tag="PLT08_18305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE06487.1"
                     /translation="MTKRIFISADHGMAIIYFLQSDVVSTLLESGVEVVVLTDDDTKD
                     KISQRFAKPGLFFEGLRLKQANDYAKKVSPRLQSLLIYLRRVGGSRRINTEAMDSHVW
                     EVWGENGWKFRLGVWIP"
BASE COUNT          332 a          321 c          381 g          312 t
ORIGIN      
        1 gagtattgaa aatgcgaaaa ggtcaaaacc ctgccaagtt cgtcaaagat gttgcccgcc
       61 ctgagcgagt gaccgttgcg ttgttaaatt acatcccctt cctgagcgga ttctatgccg
      121 agactctcga tgtgctgaag gtctcgcttg agtccatgcg caaagacgcg gggctgccgt
      181 tcgacctgat ggtcttcgat aatggctcgt gcgaagaagt gcgcgagttt ttgattggcg
      241 aaaaagaagc agggcggatt caatatttga tcctctctga aaagaacatg ggcaagggtg
      301 gcgcgtggaa tgtgatgtta gcgggcgcgc cgggtgagat cattgcttac accgatagcg
      361 acgttttgtt ctcacccaac tggctctcgc gctcggtgga gttgctcgaa accttcccga
      421 atgtgggtat ggtgacagcg cgaccgtacc gcacctcgcc ggagttgtac tcgtccacgt
      481 tggaatgggc gaagaagaat gcgactctcg atgaaggtca attcattccg tgggaaacct
      541 ttttggaatt caatctctcg cttgggcaga ccgaagaaga gaatgtgaaa gtctatgctg
      601 aaacgagtga ctggcgcatt caatacaaag gcgtgacagc gctggcaggc gcaagtcact
      661 ggcagttcac ggcgtataaa tccaccctgc agcaattcct gccctttgac atggacaagc
      721 cgatgggaca agtccgccaa ctcgataagc gcatgaacga tgcggggtta ctgcgcctga
      781 tggtcagcga tcccctggcg atgaatatgt ccaatacgct gggatatttg cgcggcgaac
      841 taaaagaaga aagaagaaag aaggtcgggg tagggaaaag ggtcttggaa ttcccgccga
      901 ttaagaagat gttgctggcg gtgtataaca agattttcag ttggtattat tcgtaggcaa
      961 ttgccaataa tggcttaacc aataaacgat atgaccaaac gtatctttat ctccgccgat
     1021 cacggcatgg caatcatcta cttcctgcaa agcgacgtcg tttcgacgct gcttgagtcg
     1081 ggggttgaag tcgtcgtcct caccgatgac gacaccaaag acaaaatctc acagcgtttt
     1141 gcgaagcccg gtttattttt cgaaggtttg cgcttgaagc aagccaacga ctatgccaaa
     1201 aaggtcagcc cgcgcttgca atctctgctc atctatttgc gccgcgtggg tggctcgcgc
     1261 cgcattaaca ccgaagccat ggacagccac gtctgggaag tgtggggcga gaacggctgg
     1321 aagttccgcc tcggggtttg gattcc
//
LOCUS       DAOQJE010000956         1335 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_102772_length_1335_cov_7.287500, whole genome shotgun
            sequence.
ACCESSION   DAOQJE010000956 DAOQJE010000000
VERSION     DAOQJE010000956.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1335)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1335)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1335
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_102772_length_1335_cov_7.287500"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..896
                     /locus_tag="PLT08_18310"
     CDS             <1..896
                     /locus_tag="PLT08_18310"
                     /inference="COORDINATES: protein motif:HMM:NF020650.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HNE06488.1"
                     /translation="ELRHIKLPMGYIPNIQYAPFYVAIDKGYFAEEGIEIEFDYSFET
                     DGVALTGAGELPFAVASGEQILLARSQGLPVVYAFAWYQQFPISIISAPELNINEPAD
                     LRGKKIGLPGLFGANYIGLEALLFEGGVDPSEVSMDAIGFNQVEAYASGNSDIVVGYA
                     ANEPIVLASQGFDLKEMRVADYVDLIANGIVTNEDTIANDPELVRGMAKALARGIEAT
                     INNPEEAYDISLKFVENLKDQDKAVQMQVLNTSIEFWKADRIGYSNPQAWENMNNVLV
                     KMALIPEPIDLSKAFTNEFIP"
     gene            952..>1335
                     /locus_tag="PLT08_18315"
     CDS             952..>1335
                     /locus_tag="PLT08_18315"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNE06489.1"
                     /translation="MTTKPILSVRNLSVTYPDNNGGLHALDNISFEVRPREFVCFLGP
                     SGSGKTTFLRVLAGILEPTAGNVNFMYHHQQKIGMVFQQANLMPWRTVIQNIMLPLEL
                     EGTGDIKSQNKAREMIKLVGLKGFED"
BASE COUNT          382 a          288 c          318 g          347 t
ORIGIN      
        1 gggaacttcg tcatattaaa ttgccgatgg gctatattcc caacatccaa tatgcaccat
       61 tctatgtagc aattgataaa gggtattttg cagaagaagg aattgaaata gagttcgatt
      121 attctttcga gaccgatggc gttgcattga cgggggcagg cgaacttcca ttcgcagtgg
      181 catcgggcga acaaatttta ttggcacgtt cacaagggtt accggttgtg tatgcttttg
      241 cttggtacca gcaatttccc atctcaatca tctctgcacc agagttgaac atcaatgaac
      301 ccgcagacct gcgcggaaag aaaattggct taccaggttt gttcggcgca aactacattg
      361 gtttggaagc attgttattc gaaggcggag tagacccatc tgaagtaagt atggatgcca
      421 ttggtttcaa ccaagtggaa gcttatgctt cgggcaatag tgacattgtt gttggctacg
      481 cagcgaacga accgatcgta ttagcatctc aaggttttga cttaaaagaa atgcgtgtgg
      541 cagattatgt tgacttgatc gcaaatggca ttgtgaccaa tgaagatacc atcgcgaacg
      601 accctgaact tgtgcgcggc atggcaaaag cactggcacg cggcattgaa gctacgatca
      661 acaaccctga agaagcctat gacatcagct tgaagtttgt agagaatcta aaagaccaag
      721 ataaggctgt gcaaatgcaa gtcttaaaca catcgattga attttggaaa gcagatcgga
      781 tagggtattc caacccacag gcgtgggaaa acatgaacaa cgtccttgta aagatggcgt
      841 tgattccaga gcctattgat ctcagcaaag catttacaaa cgaattcatc ccataaggat
      901 tttacgagtc gcggttcaca catctagtga tgtgtgaacc gtgaattagc catgaccaca
      961 aaacccatcc tcagtgttcg caacctttct gtcacttacc ccgataacaa tggcgggctt
     1021 cacgcacttg ataacatctc gttcgaagtt cgtccgcgtg aatttgtttg ttttctgggt
     1081 ccttccggct cgggaaaaac gaccttctta cgcgtgttag caggcattct cgagccaacc
     1141 gctggcaacg taaattttat gtatcaccac cagcaaaaga taggcatggt ttttcagcaa
     1201 gccaatctta tgccttggcg cactgtgatc caaaatatca tgctgcctct tgagttggaa
     1261 gggaccggcg atatcaagtc acagaataag gcacgagaga tgatcaagtt ggtgggatta
     1321 aaaggattcg aagat
//
LOCUS       DAOQJE010000957         1331 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_103482_length_1331_cov_2.634796, whole genome shotgun
            sequence.
ACCESSION   DAOQJE010000957 DAOQJE010000000
VERSION     DAOQJE010000957.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1331)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1331)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1331
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_103482_length_1331_cov_2.634796"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(264..860)
                     /locus_tag="PLT08_18320"
     CDS             complement(264..860)
                     /locus_tag="PLT08_18320"
                     /inference="COORDINATES: protein motif:HMM:NF013921.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - transposition, DNA-mediated
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HNE06490.1"
                     /translation="MPYARKNLLTWQQGMYYHIYNRGTSKATIFREESNYLFVLQKVK
                     EVCKENQLSMIAYCLEPNHYHFLVRQDGSQPAGNLPQYVFNSYTKAYNKRYEHSGTLF
                     ERRFNAKPIQTSSHLLHLCRYIHGNPVKDGLVANPADWPYSNYLEWVGERDGKLVDHN
                     FITEQFGTPEDYKGFLLEYLKTRQLPDDVIKFLGELER"
     gene            complement(966..>1331)
                     /locus_tag="PLT08_18325"
     CDS             complement(966..>1331)
                     /locus_tag="PLT08_18325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE06491.1"
                     /translation="NFVLDVLKVVAPIAAPAINVVKGPKATENWKDQFDLTKAIIDKF
                     PAEVKTPDRSLFAGKLLSEPERSGILALHALLRELDPSQANLGLYRVSTYTGDYRWLC
                     KRHYDAYQPNIPDVISGSG"
BASE COUNT          340 a          270 c          318 g          403 t
ORIGIN      
        1 cttagccaac acaaaggtga gcttggtcag cagtcaacac gccgccatca ccgatgggac
       61 cttttgggca cagttcgtgg agccagaccc ggttcatatt ttgattttga acaatgcgat
      121 tgagtttgta gtttctccat gggagaatgt ggatagggcg tatgttgggt agcggacttc
      181 ggtaattatt cgactttaga atcttatacc cattttatgg gctacagtag gtgacagtca
      241 ctttcaaagt gactgtcacc tttttatctt tctaattccc ctaaaaattt gatgacatca
      301 tcgggcaatt ggcgtgtctt gagatattct aaaagaaacc ccttgtaatc ttcaggggtt
      361 ccaaattgct cagtgatgaa attatgatca accaatttgc catctcgctc accgacccat
      421 tctaaataat tcgagtatgg ccaatctgcg gggttggcga ccagaccatc tttgaccggg
      481 ttgccatgga tataacggca aagatgcaaa agatgactgc tggtttggat cggttttgcg
      541 ttgaagcgtc gttcaaatag tgttccgctg tgttcatatc gtttgttata ggcttttgtg
      601 taactgttga atacatattg aggcaagttt ccagcgggct gacttccatc ctgccggacg
      661 aggaagtgat agtgattggg ttcaagacag tatgcgatca tactgagctg attttctttg
      721 catacctctt taaccttttg gagtacaaaa agataattgc tctcttcacg aaaaatagtc
      781 gccttgctgg tgccgcgatt gtagatgtgg taatacatcc cttgttgcca tgtgagtaga
      841 tttttgcgag catagggcat gggtttccct cctaataagt gagatatatc tttattattt
      901 tacttctact tagaatgcgg cgtgaaaaaa gtgacagtca cctttaaggt gactgtcact
      961 tgggtttagc cacttccact gatcacatca gggatattcg gctgatacgc atcgtagtga
     1021 cgtttgcaca gccagcgata atcgccggtg taggtggaga cgcggtaaag accaaggttg
     1081 gcttgggatg ggtctaattc tctgagcaag gcatgtaagg caaggatgcc agaacgctca
     1141 ggctcactaa gcagtttccc tgcaaataag gaacgatcag gcgtttttac ttctgctggg
     1201 aatttgtcga tgatcgcttt agttaggtca aactgatctt tccaattctc agtggctttt
     1261 ggacctttga caacattaat ggctggagcg gcaataggag ccactacttt caaaacatct
     1321 aatacaaaat t
//
LOCUS       DAOQJE010000958         1330 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_103593_length_1330_cov_6.198431, whole genome shotgun
            sequence.
ACCESSION   DAOQJE010000958 DAOQJE010000000
VERSION     DAOQJE010000958.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1330)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1330)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1330
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_103593_length_1330_cov_6.198431"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..816
                     /locus_tag="PLT08_18330"
     CDS             <1..816
                     /locus_tag="PLT08_18330"
                     /inference="COORDINATES: protein motif:HMM:NF024579.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNE06492.1"
                     /translation="IDQALAADPTLMDVHRVHAYVLESLGEYALAIEAYDKAIAIAPN
                     MTFLYLRAGAGYRRLAFESANDEVQRQLFEKSLEYFAQTARINSQLGVKDPAPYISIA
                     KTYSQMGEFFIAIRNIQKAIEFEPANAVLYGELGVLYHKNRNYETGILALGCAINGCT
                     GPESCDGRGGCGPNDVEAEVKPLALDGSTVYYYDILASELAALSTPKNNKCPQALEVA
                     AIIENSEYISDPNIATDMAVVRTICSFSPETTNTGSEPTPTAESATPEVTPTP"
     gene            1066..>1330
                     /locus_tag="PLT08_18335"
     CDS             1066..>1330
                     /locus_tag="PLT08_18335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003966899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HNE06493.1"
                     /translation="MAKNNVSFKPLGSRVLIETIEQEEITASGIILPETAKEKPQQGK
                     VLAAGPGDRNDKGERVAMDVKTGDVVLFAKYSGTEVKMDGKKLL"
BASE COUNT          348 a          295 c          362 g          325 t
ORIGIN      
        1 atcgatcagg cattggcagc tgaccccaca ttgatggatg tgcaccgtgt gcatgcctat
       61 gtgttggaat ctttgggcga atatgcgctc gcgattgaag cgtatgataa agcaattgcg
      121 attgcaccaa atatgacatt tctttacctg cgggcagggg cagggtatcg ccgtcttgct
      181 tttgaaagcg ctaatgacga agtgcaaaga cagttgtttg agaagtcgct tgaatatttt
      241 gcacagacgg ctcgtatcaa ttcgcagttg ggtgtgaaag acccagcgcc ctatatttcc
      301 attgcaaaaa cctattcgca aatgggtgag ttcttcattg ccattcgtaa tatccaaaag
      361 gcaattgagt tcgagcctgc taatgccgtt ttgtatggcg agttgggcgt gttgtatcac
      421 aagaaccgta actatgaaac cggtattctc gccctagggt gcgcgattaa tggttgtacg
      481 ggacctgagt cttgtgatgg gcgcggtggc tgtggtccca acgacgtgga agctgaagtg
      541 aagccattgg cattagatgg cagtacggtt tattactacg atatccttgc ttctgagttg
      601 gcggcgttga gtaccccgaa aaataataaa tgccctcagg cgctagaggt tgcggcaatt
      661 atcgaaaact ctgaatatat ttcagacccg aacattgcta cggatatggc tgtggttcga
      721 acgatctgtt cgttctctcc tgagacaaca aacaccggct ctgagcctac accgacggct
      781 gaatcagcca cccctgaggt gacgcccaca ccttgattga cgagagggtc ctagcgcctg
      841 ccagagccag ggtacagcgg gggtaaggct cgggtaggcg aggcagaaag cgggtccaga
      901 aagaggcggg cgaagcgtca tcagagaaat gtaagaattt ttatatatcc ccttgacgtt
      961 gactttttaa ctgtgtaaga tagactttag cactcagttg tattgagtgc taattctgtg
     1021 attatcctaa aaaatcaaaa gtatcaacct tacaggaggc atcaaatggc aaagaacaat
     1081 gtttcgttca aacccctcgg cagccgcgtg ctgatcgaga ccattgaaca ggaggaaatc
     1141 accgcaagcg ggatcatcct ccccgaaacc gcgaaggaaa aaccgcagca gggcaaggtc
     1201 ttggctgctg gtcctggtga ccgcaacgac aagggcgagc gcgtcgccat ggatgtaaag
     1261 actggtgatg tagttttgtt cgccaagtat tctggcaccg aagtgaagat ggatggcaag
     1321 aaattgctca
//
LOCUS       DAOQJE010000959         1321 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_105027_length_1321_cov_2.888626, whole genome shotgun
            sequence.
ACCESSION   DAOQJE010000959 DAOQJE010000000
VERSION     DAOQJE010000959.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1321)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1321)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1321
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_105027_length_1321_cov_2.888626"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..463)
                     /locus_tag="PLT08_18340"
     CDS             complement(<1..463)
                     /locus_tag="PLT08_18340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE06494.1"
                     /translation="MYFNMRKLKLIFAWLMILMAMLACKSSSMEREDAHISCQDHFYK
                     KSNGEVGVKASNCTYTCDNDTVLSRVFDENTPPEQSEILFYCNQPAASVAEESVATEP
                     PTEEVPTEAPTDAPTEAPTEAPSAEPPAPILTGDVTYCDIVSRSVNLRLVEG"
     gene            636..>1321
                     /locus_tag="PLT08_18345"
     CDS             636..>1321
                     /locus_tag="PLT08_18345"
                     /inference="COORDINATES: protein motif:HMM:NF027681.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HNE06495.1"
                     /translation="MKYFVTGATGFVGGVLAKKLREAGHEVNASVRSPEKAKDLQALG
                     VKLFKGDVTDKESMREAMTGVDGVYHIAGWYKVGTRDKSGGVQVNINGTRNVLELMQE
                     LKILKGVYTSTLAINSDTKGVLADEKYHFTGEHISEYDRTKAEAHKLADEFIAKGLPL
                     VIVQPGLIYGPGDTSSVRASLLDLLKGRLPMLPRQTAMSWAHVDDIVHGHILAMEKGK
                     VGESYIICGE"
BASE COUNT          378 a          296 c          319 g          328 t
ORIGIN      
        1 atccttccac cagacgcagg ttcacagatc gagaaacaat atcgcaatag gttacatcac
       61 cagtgaggat cggagcggga ggttcagcgg acggcgcttc tgtcggcgct tctgtaggag
      121 catcagttgg tgcctcggtg ggaacttctt ctgttggtgg ttctgtagca acagattcct
      181 ctgcaacgga tgcagcaggc tgattgcaat aaaataaaat ttcagattgt tctggtggag
      241 tattctcatc gaagacccta gaaagcacag tatcattgtc acaagtatat gtgcaatttg
      301 atgccttaac tcccacttcg ccattgcttt ttttataaaa atgatcctga cacgatatgt
      361 gtgcatcttc ccgttccata gatgatgact tacaagccaa catcgccatt agtatcatta
      421 gccatgcaaa tatcaacttt agtttcctca tattgaaata catcttcgtt cttctcctct
      481 tttttgatat gcatccgatg acttagaaat tattatagca aaagtagata attattgtct
      541 gacaaatcca tattcccagt aaaatggaag ctgaaacgta aagttccttt caattcgcac
      601 gtctgcactt caataaccgc aataggagaa tgaagatgaa atattttgta acaggcgcaa
      661 ccggcttcgt tggcggagtt ttggcaaaaa aactgcgcga agctgggcat gaggtcaatg
      721 cctccgtccg ctcgccagag aaggcgaaag acttgcaagc cctcggcgtg aaattgttca
      781 aaggcgatgt aaccgacaaa gagtctatgc gcgaggcgat gaccggtgtg gacggggttt
      841 atcacattgc tggctggtac aaagttggca caagagacaa aagcggcggg gtacaggtca
      901 atatcaacgg cacacgcaat gttttggaat tgatgcagga attgaagatc ctcaaaggcg
      961 tgtacaccag cacactggcg atcaactcag ataccaaggg tgttctggca gacgagaagt
     1021 accacttcac aggtgaacac atcagtgaat acgaccgcac caaagcggaa gcgcataaat
     1081 tggcagatga attcatcgcg aagggactac ccttggtcat cgtccagcca ggattaatct
     1141 atggaccggg cgatacatca tcggtccgcg cctctttgct ggacctgttg aagggtcggc
     1201 tgcccatgtt gcctcgtcaa accgccatga gctgggcaca cgtagacgat attgtgcacg
     1261 gacacatact agcaatggaa aaaggaaaag tcggcgaaag ctatattatt tgcggtgaaa
     1321 a
//
LOCUS       DAOQJE010000960         1310 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_106840_length_1310_cov_2.131474, whole genome shotgun
            sequence.
ACCESSION   DAOQJE010000960 DAOQJE010000000
VERSION     DAOQJE010000960.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1310)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1310)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1310
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_106840_length_1310_cov_2.131474"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..805)
                     /locus_tag="PLT08_18350"
     CDS             complement(<1..805)
                     /locus_tag="PLT08_18350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE06496.1"
                     /translation="MRNRNTNTILRGVSILFLLGAFTLTVISLISYSRQRTGYPAGMM
                     VAGVPVDGLSPAEASQRILQVYNSPIEVNYNGAVFHIDPGAVGFTMDIETMLAAADLT
                     RTGGSFWGGFWDYLWNRPPTEAEIPLSATISEDRLREFLQNEIASRYDIPPAPAQPVP
                     GGTTFLPGAPGQTLDINRAVVIITNSLRSPADRSVVLTFNQTTASRPTIENLEILLKQ
                     IITTSDFDGLIGYYMMDLQTGQEIHFLINNKQEITVEPDVAFTASSTIKL"
     gene            932..>1310
                     /locus_tag="PLT08_18355"
     CDS             932..>1310
                     /locus_tag="PLT08_18355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE06497.1"
                     /translation="MLRSFFIYLSKAAWAQKLITSWSFSWRIASRFVAGVTLADAIKT
                     VRELNAKGINATLDHLGEHTNTPEEAQQAADSIFATLDAIGAEPSLRSNISIKLTQIG
                     MGLDESLCVETLERILARAKTAKT"
BASE COUNT          358 a          270 c          343 g          339 t
ORIGIN      
        1 gtaacttgat cgtactggat gcagtaaagg ctacatctgg ttctactgta atttcttgtt
       61 tattgttgat caaaaagtga atttcttgcc cggtttgcaa gtccatcatg tagtaaccga
      121 ttaaaccgtc aaagtctgat gtggtgatga tttgcttgag caagatttcc agattttcga
      181 tagttgggcg agacgctgtg gtttggttaa aggtcaacac aacagagcgg tctgcaggag
      241 aacgcaaaga attagtaatg atcacaacag cacggttgat gtcgagagtc tgtccaggag
      301 cgccgggaag gaaggtggtg ccgccgggga cgggttgcgc aggagcaggc ggaatatcgt
      361 agcgtgatgc aatctcgttc tggaggaatt cgcgcaaacg atcttcggat atggttgcgc
      421 ttaatgggat ttctgcttca gttggcggtc ggttccagag gtagtcccaa aacccgcccc
      481 agaaagagcc gccggttctt gtcaggtctg cggcagcaag catggtttca atatccatcg
      541 tgaagccgac tgcaccaggg tcaatgtgaa agactgctcc attgtaattt acctcaatag
      601 gcgagttata gacctgaaga atcctttgtg acgcttcagc aggggacaag ccgtctacag
      661 gtacgccggc aaccatcatg ccagcagggt agcccgttct ttggcggcta taggaaataa
      721 gggaaataac ggttagtgtg aatgccccaa gtaaaaatag gattgaaaca ccccgaagga
      781 tggtgttggt atttcgattt ctcacggtta aacattgcct ccaatgtcaa tggcatgaca
      841 aaaatcacgg aaaaagtata acataccgaa acatgaggct tgtgctagaa taagttgata
      901 ttaatctaaa atcatagttt aaaggatgga tatgctccgt tcatttttta tctacctttc
      961 gaaagctgcc tgggcgcaaa agctaatcac aagttggagc ttttcttggc gcatcgcttc
     1021 tcgttttgtt gcgggggtta cactcgcaga tgctataaag accgtccgtg agttaaatgc
     1081 aaaggggatc aacgctacct tagatcactt aggggaacat acaaatacac ctgaagaagc
     1141 tcaacaggca gccgacagta ttttcgcaac cttggatgcc attggagcag agcccagcct
     1201 tcgtagtaat atttcgatta agttgacaca gatcggcatg gggttggatg agagtttgtg
     1261 tgttgaaacc ttggaacgta tcctcgcacg tgccaaaact gcgaagactt
//
LOCUS       DAOQJE010000961         1308 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_107158_length_1308_cov_2.276935, whole genome shotgun
            sequence.
ACCESSION   DAOQJE010000961 DAOQJE010000000
VERSION     DAOQJE010000961.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1308)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1308)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1308
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_107158_length_1308_cov_2.276935"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1077)
                     /locus_tag="PLT08_18360"
     CDS             complement(<1..1077)
                     /locus_tag="PLT08_18360"
                     /inference="COORDINATES: protein motif:HMM:NF012722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase dimerization/phospho-acceptor
                     domain-containing protein"
                     /protein_id="HNE06498.1"
                     /translation="MLSRIPQWIRSPIFTEDDNKTLSANLLNIIIWVFIIGSSLYGLT
                     APIGTDFFIRRIFFIIPFILVMLAAKQTLNWGYIQLSGNLVVISIWTLITSALFMGAG
                     YQNPAYMGYIVVAICAGLILNRWATISWVFVCIITSAVILNLGTQGFLPEYRIPTSPF
                     SFWSAQTVYILVSSVLLSQTLQKIEDARNRAKLELEERKRVETKHELVIRELEIKNAE
                     LERFIYTVSHDLKSPLITIGGFIGLLEKDMHTNDIVKIKNSIERIREAKDKMSYLLDD
                     LLELSRIGRLKNPSINISLRDIVDEALSLVHGQLTARKIQVQIQETLPTLYCDSPRLV
                     EVIQNLVDNAAKFMGEQPNPVIEIG"
BASE COUNT          366 a          273 c          283 g          386 t
ORIGIN      
        1 tccaatttcg atcacaggat ttggttgttc tcccatgaat ttggcggcat tatccacaag
       61 gttttgtatc acctccacaa gccgtggaga atcacagtat aaagttggaa gggtctcctg
      121 tatttgtact tgaatcttgc gggcggttaa ttgtccatgc accaaactca gcgcttcatc
      181 cacaatgtcg cggagggaaa tattgatgga tgggtttttc aaacgtccaa tgcgtgaaag
      241 ttcaagcagg tcatccaaga gatacgacat cttgtctttt gcttcacgta tgcgctcaat
      301 ggaatttttg attttgacaa tgtcgtttgt gtgcatgtct ttctccagca atccaataaa
      361 cccgccaatc gtaatcaaag ggctttttag atcgtgcgac acagtgtaga tgaagcgttc
      421 caattccgcg ttcttaattt caagttctcg aatgaccaat tcgtgtttgg tttccacccg
      481 ttttctttct tccaattcca gttttgctcg attacgggca tcttcaattt tctgaagagt
      541 ttgtgataag agaactgaag agactaaaat gtacaccgtc tgcgctgacc aaaaggaaaa
      601 tggggatgtt ggaatcctgt actcgggcag aaagccctgt gttcccaaat taagaataac
      661 cgcactagta ataatgcata caaacaccca gcttatcgtt gcccatctat ttaaaataag
      721 accagcgcag atggctacta caatataacc catgtacgca gggttttgat atcctgctcc
      781 cataaagagg gcactggtga ttaatgtcca tatggaaata accacaagat tgccggataa
      841 ttgaatatag ccccaattga gagtttgctt tgccgctagc atgacgagaa taaagggaat
      901 gataaagaat attcgcctta tgaaaaaatc cgtcccgatt ggcgctgtca acccgtagag
      961 actggatcca atgataaaga cccaaataat gatgttaagt agatttgcag aaagagtctt
     1021 gttatcatct tctgtaaata tgggcgatct tatccattgg gggattctac tcaacatgcg
     1081 ctgctcctga cacgaacaat agtccaacag gatgattata taggcattgg cgcgattcca
     1141 ctattaggta tcaaacaaca aacccgccta ttgtgaaagg cgggtttgtt gtttgatatt
     1201 ttgtctatac tgggactttg tcccattctt tttgagcgtg ttcaaatccg cgtttcagag
     1261 cctcgaagtt ctttggcaag agatgtttgt ggcgagcagg taaatgac
//
LOCUS       DAOQJE010000962         1305 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_107594_length_1305_cov_2.575200, whole genome shotgun
            sequence.
ACCESSION   DAOQJE010000962 DAOQJE010000000
VERSION     DAOQJE010000962.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1305)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1305)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1305
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_107594_length_1305_cov_2.575200"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            88..1065
                     /locus_tag="PLT08_18365"
     CDS             88..1065
                     /locus_tag="PLT08_18365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE06499.1"
                     /translation="MDQSNSKPSKIWNTVTQLATFLGLIASIAAVISVIVQVKSESRL
                     LEIQIVSADQLTSLPNVDGLKGEFSYKDVSVSDLWRLKLQIVNSGDVTLIGEGQTSSL
                     LHGSIPIQFPSGVVILDVSEKVGEFYVINQKTPDQIEITFSQWRPKEALETVIYITSD
                     TVLKSAPTPTISGRPIIDGNIIVLEPSSQGNLGQQPFLDKLPSPLPITARILGVIFTV
                     VVFCLIFYSTIISFPIQYAKASAWKNKNNAVFVNHLYKLGLKPDEIKTYTDKPWRFKY
                     MNTIQKMKIDLEFPDFPTFETFWEVLMWFIAGILLCIICVGVVAGLIIV"
     gene            complement(1051..>1305)
                     /locus_tag="PLT08_18370"
     CDS             complement(1051..>1305)
                     /locus_tag="PLT08_18370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE06500.1"
                     /translation="LSLQRAERKERKFVCSFRNVMDCFCEFIFCAPAPNGLRYWLVGG
                     TLTRSTGRKMFGTLIMLEKPTVPTSPVHALLGVVVKLHNY"
BASE COUNT          425 a          251 c          248 g          381 t
ORIGIN      
        1 tggctggacg gcttcgccgt ccccgcccca gcgcaggtaa cgcaaaccgt taggcgttta
       61 atagaaaaga aaagagtata aagccatatg gatcaatcaa attctaaacc ttcaaaaata
      121 tggaataccg ttactcaact tgctactttt ttgggtttaa tagcatccat agcagcagtg
      181 attagcgtca tcgttcaagt aaaatcggag agtaggttat tagaaattca aatagttagc
      241 gcagatcagt taacatcatt acctaatgta gatgggttaa agggtgagtt ttcttataaa
      301 gatgttagcg tatcagacct ttggaggctc aaattacaaa ttgtaaatag cggagatgta
      361 accttaattg gtgaaggaca aacaagttcg cttttacacg gtagtattcc aatacaattt
      421 ccatcagggg ttgtcattct tgatgtaagc gaaaaagtgg gtgagtttta tgtaataaat
      481 caaaaaaccc cagaccaaat tgaaattaca ttctctcaat ggagaccaaa agaggcgctg
      541 gaaacagtaa tatatattac atcagatact gttttaaaat ctgcgccaac cccaacaatt
      601 tctggtagac caattattga tggaaatatc attgtcctag agccctcctc gcaagggaat
      661 ctaggtcagc agccgttttt agataaatta ccaagtccat tgccaataac agcacgtata
      721 ttaggtgtta tttttactgt ggtcgttttt tgtttaattt tttacagcac aatcatttca
      781 ttcccaattc aatatgcaaa agccagtgct tggaaaaata aaaataatgc ggtcttcgta
      841 aatcatctat ataaattagg attaaagcct gacgaaataa aaacatatac agataagcct
      901 tggcgtttta aatacatgaa cacaatacaa aaaatgaaaa ttgatctaga gtttcctgat
      961 ttcccaacgt ttgaaacatt ttgggaagtt cttatgtggt ttatagcagg catactttta
     1021 tgcatcattt gtgtaggtgt tgttgcagga ttaataattg tgtaatttta caactacgcc
     1081 caacaaagcg tgcactggac ttgtgggtac ggtgggcttt tcaagcatta tcagcgtccc
     1141 gaacattttt ctgcccgtcg agcgagtcag cgtcccgccc acaagccagt aacgcaaacc
     1201 gttgggcgct ggcgcacaaa aaataaattc acaaaaacaa tccatcacat tcctaaatga
     1261 gcacacaaac ttcctctcct ttctttcggc tctttgtaaa gatag
//
LOCUS       DAOQJE010000963         1302 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_108033_length_1302_cov_4.141139, whole genome shotgun
            sequence.
ACCESSION   DAOQJE010000963 DAOQJE010000000
VERSION     DAOQJE010000963.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1302)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1302)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1302
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_108033_length_1302_cov_4.141139"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            15..>1302
                     /locus_tag="PLT08_18375"
     CDS             15..>1302
                     /locus_tag="PLT08_18375"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HNE06501.1"
                     /translation="MAETPLWQKILFSLVALLVMVFSGYGAYQISKRNHAGRMISLTI
                     DYLGFVICFVFALNVGEFFIGIDALGENFGRGIPYLGITALGYFLGMLEEYFPTKNKD
                     SESSLKTVSKWVMLGGFVLFLWQVNALNGLIYFLGKLAQPSGIVAVVGLVIFGISLWS
                     MSQPNIAQALNVKTGHEQVINGWLFLSPNLLGFLIFFAGPLLLSFYFSFTDSDAIRTP
                     NWVGFENYAKILNIKFVTLDTPDQLARDAVDIKVYDEVGRFKFGDTGILFAAEDKFFW
                     LALRNTLTFVLFAVPLSVIPALILSNILNSKLPGMKFYRAVYFMPSIAAVVGISLVWQ
                     WLYNATIGYINYFITLGIEFWNNLFNVAVVDPKIQWVSDEKTALFAIIIIAAWQTMGF
                     NTVLFLAGLQNIPGELYEAATVDGAGAWDKFWGITLP"
BASE COUNT          280 a          312 c          316 g          394 t
ORIGIN      
        1 caatctggca gggaatggca gaaacacccc tatggcaaaa gattctattt tcgctggttg
       61 ctttgttagt tatggtcttc agtggatatg gggcatacca gatctcaaaa cggaatcatg
      121 ccggtcgcat gatctccttg accattgatt acctcgggtt tgtcatttgt tttgtattcg
      181 ctttgaatgt aggcgaattt tttattggca tcgatgctct cggcgaaaat tttggtagag
      241 gcattcctta tcttggcatc actgccttgg gctacttcct cggtatgttg gaggaatatt
      301 tcccaactaa gaataaagac tcagaaagca gcctcaagac cgtcagcaag tgggtgatgt
      361 tgggcgggtt tgtgctcttc ctctggcagg tgaatgcgct caatgggctg atctatttcc
      421 tcggcaaact tgcccaaccc agtggaatag tcgccgttgt cggtttggtg attttcggca
      481 tctcgctttg gtctatgagc cagccgaaca ttgcacaagc cttgaacgtc aagaccgggc
      541 acgagcaggt tatcaacggc tggttgttcc tttcgccaaa tttgctgggc tttcttattt
      601 tctttgcggg tccgttactg ctttcgttct atttctcatt cacagactca gatgccatcc
      661 gtacgccgaa ttgggtcggg tttgaaaatt acgccaagat cctcaatatc aaatttgtca
      721 ccctcgacac acccgatcaa cttgcccgtg atgccgtaga tatcaaagtc tatgatgaag
      781 tagggcgctt taaatttggg gatacgggca ttctgtttgc tgctgaagat aaattcttct
      841 ggctcgcgct tcgcaacacc ctgacctttg tgctgtttgc tgttcccttg agcgtgatcc
      901 ctgctttgat cttatcgaat atcctcaata gcaaattacc tggtatgaag ttctatcgcg
      961 ctgtgtattt catgcctagc atcgcggcag tcgtaggtat ctcgctcgtt tggcagtggc
     1021 tatacaacgc gaccatcggg tatatcaact actttattac cttgggcatt gagttttgga
     1081 ataatctgtt caatgttgct gtcgttgacc cgaagatcca atgggtttcc gatgaaaaga
     1141 cagcactctt tgccatcatc atcatcgccg cttggcagac gatgggcttc aacaccgtgc
     1201 ttttcctcgc gggcttgcag aacatccccg gtgagttata cgaagctgcc accgtggacg
     1261 gagccggagc ctgggacaag ttctggggca ttacgcttcc ca
//
LOCUS       DAOQJE010000964         1300 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_108400_length_1300_cov_3.033735, whole genome shotgun
            sequence.
ACCESSION   DAOQJE010000964 DAOQJE010000000
VERSION     DAOQJE010000964.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1300)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1300)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1300
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_108400_length_1300_cov_3.033735"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..769
                     /locus_tag="PLT08_18380"
     CDS             <1..769
                     /locus_tag="PLT08_18380"
                     /inference="COORDINATES: protein motif:HMM:NF020650.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HNE06502.1"
                     /translation="LIPVASAPERDQLLAAGQADGTINETLAVMLFNKDAVQMQVVRY
                     ALRPGEGYPHFRILASNQSSITQSTELKGVEIGVSQGTIIEYVTERLLKTQGLAPDEI
                     KTIAVPKIPDRMALLGSGELSAAVLPDPLASLAMSQGAVNVLDDSMNPEYGFSVYSFR
                     KDVIDANPEAVKAFLAAIEEARSLINPNPSKYTSLLSDKGLIPPPLLETFRVPPFPQS
                     EVPTEAEWNDGLAWLKEKGILSVDVSYADSVNSSLLP"
     gene            815..>1300
                     /locus_tag="PLT08_18385"
     CDS             815..>1300
                     /locus_tag="PLT08_18385"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNE06503.1"
                     /translation="MISIQSLTFEYNHHSPLFQNFNWTVSCGETWSILGPSGCGKTTL
                     LYLLAGLKFPTSGQVSIHGEKIQRPRPHSGLILQDYGLLPWSTVRENVALGIKVRTFY
                     GADGKHTPNNFTPKDNIESWIERLGLSEVADKFPSQLSGGQCQRTAIARTLALEPDLL
                     LM"
BASE COUNT          345 a          322 c          306 g          327 t
ORIGIN      
        1 attgattcca gtggcatcgg cacctgaacg agaccagctt cttgctgcgg gtcaggcaga
       61 tggcacgatc aatgaaacgt tagcggtaat gcttttcaac aaagatgccg tgcaaatgca
      121 ggtagtgcgt tatgccttgc gccctggtga agggtatcca cattttcgta tccttgcttc
      181 aaaccagagt agcattacac aatccactga attgaaggga gtagagattg gcgtctcaca
      241 aggaacaatc attgaatatg tcaccgagcg tttgctaaaa acacaaggtc ttgctcctga
      301 tgaaattaag accatcgctg tgccaaagat tccagaccgt atggcattgc ttggttcagg
      361 tgagctttct gccgcagtgt tgcctgaccc acttgcctca cttgccatga gtcagggtgc
      421 ggtcaatgtt ctggatgact ccatgaaccc tgaatatggg tttagtgtct attcgttccg
      481 caaagatgtt atcgacgcga accctgaagc cgtaaaagca ttccttgcag caatcgaaga
      541 agcaaggtct ctgatcaacc caaaccccag caaatacact tcactattaa gcgataaggg
      601 actcatcccg ccgccactgt tggaaacatt ccgcgtgccg ccctttccac aaagtgaagt
      661 gccaactgaa gccgaatgga acgatggttt ggcatggctc aaagaaaaag ggattttgag
      721 cgttgatgta agttacgcag attcggtcaa tagttcgctg ttgccctaac aaatcaattg
      781 gcagccacct ttagtggctg ccaatttcca tctcatgata tccattcaat cactcacctt
      841 cgaatataac catcactcac cgctcttcca gaatttcaac tggacagttt catgcggcga
      901 gacatggtct attctgggtc catctggatg tgggaaaacg actttgttgt atctgttggc
      961 aggtttgaaa ttcccaacct ctggccaggt gagtatccat ggagagaaaa ttcaacgacc
     1021 gcgtccacat agcgggttga tactgcaaga ttacggcttg ctgccttggt ctaccgtgcg
     1081 cgaaaatgta gcactcggca taaaagtccg caccttttat ggggcagatg gcaaacacac
     1141 gcccaataac ttcaccccga aagataatat tgaaagttgg atagaacgcc ttggattaag
     1201 cgaagttgca gataaatttc ccagccaact ttctggtggg caatgtcaac gcaccgccat
     1261 tgcgcggacg ctcgcgcttg aaccggacct gcttctgatg
//
LOCUS       DAOQJE010000965         1298 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_108701_length_1298_cov_4.633950, whole genome shotgun
            sequence.
ACCESSION   DAOQJE010000965 DAOQJE010000000
VERSION     DAOQJE010000965.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1298)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1298)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1298
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_108701_length_1298_cov_4.633950"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            309..923
                     /gene="tmk"
                     /locus_tag="PLT08_18390"
     CDS             309..923
                     /gene="tmk"
                     /locus_tag="PLT08_18390"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HNE06504.1"
                     /translation="MFITLEGPEGSGKTSHIPHLVEFLREKGYTVFPTREPGGTSISE
                     QIRDVLHDMKNAEMHARTETLLYQAARAQIVEQVIKPRLADGEIVISDRYYDSTIAYQ
                     GYGHQQNLDEIRALVKYATGGLTPDLTILLDLDVEVGLGRKKKDNEWNRLDAYTVEFH
                     RRVRAGYQEMVKAEPQRWAVVNSEQAWDSVQAELRQVILEKLGK"
     gene            976..>1298
                     /locus_tag="PLT08_18395"
     CDS             976..>1298
                     /locus_tag="PLT08_18395"
                     /inference="COORDINATES: protein motif:HMM:NF015534.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Type 1 glutamine amidotransferase-like
                     domain-containing protein"
                     /protein_id="HNE06505.1"
                     /translation="MPSNLHLFSVPGERDLDDIVDACRPYVEGKEEPTIAYLPLASLY
                     AERWLEMTEDAFKGLAELKTINTELMTQKAIEGIIRDADVVYVPGGNTFLLNHRLHAS
                     GVMPFL"
BASE COUNT          343 a          314 c          316 g          325 t
ORIGIN      
        1 gtttcagcag cggatttttg attgttctgc ttcacgccta ttgcctgata gacagcaaac
       61 ccgccagccg ctgcacctgt cagtgctgag acgccagcaa tcacgacaac cagaaacaaa
      121 tagacaatat ttttctttga cattcatgcc tccaagttga aaaaatcgta gtcctcgaaa
      181 atgacacgat gattaaaaat tttcacctca aactgaaagc aggttaagaa agatgtagga
      241 aaaatgtaaa cacttaaccg gctcgttttc ctgtttactt gttaacttgt ctactttata
      301 cttgccccat gttcattact cttgaaggtc ccgaaggctc gggcaaaacc tcccatattc
      361 cacaccttgt tgagtttcta cgcgagaagg gctataccgt ctttccaacc cgcgaacccg
      421 gcgggacgtc catcagcgaa cagattcgcg atgtgttgca tgatatgaag aatgcagaga
      481 tgcatgctcg cacggagacg ttgctctatc aagccgcacg cgcccagatc gtggagcagg
      541 tcatcaaacc gcgtcttgcc gacggggaga tcgtcatttc ggatcgttac tatgactcaa
      601 ccatcgccta tcaaggctac ggtcaccagc aaaacttgga tgaaatccgc gccttggtta
      661 aatacgccac cggcgggttg actccagatt tgaccattct gctcgacctc gatgtagaag
      721 ttggtttagg gagaaagaag aaggataacg aatggaatcg cctggatgct tacacagttg
      781 aatttcatcg ccgtgtccgc gccgggtatc aagaaatggt gaaggctgag ccgcaacgtt
      841 gggcagtggt caactcggag caagcatggg attccgtgca ggcggagttg aggcaagtga
      901 ttttagaaaa attaggtaaa taatgtcgta ggggcggggt caccccgccc ctacatcttt
      961 gatacaatcc ccgttatgcc atccaatctt catctctttt ctgtccccgg agaacgggac
     1021 ttagacgata tcgtcgatgc ttgccgtcca tatgtggaag gcaaagagga accgaccatt
     1081 gcctatttgc cgttggcttc tttatatgcc gaacgttggt tggaaatgac cgaagatgcc
     1141 ttcaaggggt tggcagaact gaaaacgatc aacaccgagt tgatgaccca aaaggcaata
     1201 gaaggtatta tccgcgatgc agacgttgtg tatgtgcctg gtgggaatac ctttttgcta
     1261 aaccatcgtc tacatgctag cggggtaatg cctttcct
//
LOCUS       DAOQJE010000966         1294 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_109380_length_1294_cov_7.443906, whole genome shotgun
            sequence.
ACCESSION   DAOQJE010000966 DAOQJE010000000
VERSION     DAOQJE010000966.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1294)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1294)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1294
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_109380_length_1294_cov_7.443906"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..991)
                     /locus_tag="PLT08_18400"
     CDS             complement(<1..991)
                     /locus_tag="PLT08_18400"
                     /inference="COORDINATES: protein motif:HMM:NF013106.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HNE06506.1"
                     /translation="MTSTLPVLRARRERRLEKQKRNENRSRGAILSVGLILSLVLGGL
                     IILGAFAYSDVTRDLPSPQILRRLLNPPDGLLLQPTRVYDRTGLQLLATFAPSESPRR
                     YIPISEQNAQHVPAEMVKALIAVADPSFETHSGYSLEGFNNYDMHPTIAQKLVNALML
                     YDEPPSLKRAIRERLLAVQITSQFGRTQIMEWYLNSADFGNYAYGIDAAAQLYFGKPA
                     DELTLAESAILAATSETPALNPHDAPQVAIQRGRELLYVMSDLGLVTAEQVDPALAET
                     PTFTEAPQTPAEVAPAFLNLLLAQVDSQIPRDRIERGGITVISTLDYDLQKDAA"
BASE COUNT          338 a          297 c          358 g          301 t
ORIGIN      
        1 aggcagcatc tttttgcaag tcataatcta atgtagagat gaccgtaatg ccaccgcgct
       61 caatacggtc gcgcggaatt tgagagtcga cttgggcgag caataagttt agaaacgctg
      121 gagccacttc ggctggagtc tgaggcgctt ctgtgaatgt gggcgtctca gccaaggctg
      181 ggtccacttg ctcggcggtg acgagtccaa ggtcgctcat tacatataaa agctcacgtc
      241 cgcgttgaat ggcaacttgc ggagcatcat gcggattcag ggcaggcgtt tcactggttg
      301 ccgccaagat cgcagactct gccagagtta gttcatcggc aggtttcccg aaataaagtt
      361 gagcggcagc atcaatacca taggcataat tgccaaaatc agcgctatta agataccact
      421 ccatgatctg tgtgcgcccg aactgagatg tgatctgcac cgcaagcaaa cgctcgcgaa
      481 tggcgcgctt cagagatggc ggttcgtcgt acaacatcaa ggcgttgacc agcttttgcg
      541 caatggtggg atgcatatca tagttattaa acccttcgag cgaatagccc gaatgcgttt
      601 cgaaacttgg gtctgcaacc gcaatgagtg ccttcaccat ttcggcggga acatgctggg
      661 cattttgttc gctgatggga atgtaacggc gcggagattc actgggcgca aaggtagcta
      721 acaattgcaa accggttcgg tcgtagaccc gtgttggctg tagcaaaagc ccatcgggcg
      781 ggttgagcag gcgtcgcaaa atctgaggcg aaggcaaatc gcgggtgaca tccgaataag
      841 cgaaagcacc caagataata agcccgccca acaccagaga gaggataagc cccacactga
      901 gaatggcgcc gcgcgaacgg ttctcgttgc gcttttgttt ttcaagcctg cgctcacgcc
      961 gcgcacggag gaccggtaaa gtagaagtca tacaggtagt atattgggat tttgaaaatt
     1021 ggtaaatcgg gaattgtcag tttggtgttt tataatgaca aggtttactg gacaattaag
     1081 catgttcgcc tgcaacgcat ctcaatttat tggagacttt catgacacca aatatcttta
     1141 tgagttattc aaggcgcgaa acagggtttg tagatgacct aacccatagg ctggagaaag
     1201 ccggttttaa cgtctggctc gattaccgaa ggttaattcc aggcacgccg tgggctgggc
     1261 aaattcaaaa aggcttgcaa gaagcagaag tggt
//
LOCUS       DAOQJE010000967         1294 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_109433_length_1294_cov_2.752220, whole genome shotgun
            sequence.
ACCESSION   DAOQJE010000967 DAOQJE010000000
VERSION     DAOQJE010000967.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1294)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1294)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1294
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_109433_length_1294_cov_2.752220"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1294
                     /locus_tag="PLT08_18405"
     CDS             <1..>1294
                     /locus_tag="PLT08_18405"
                     /inference="COORDINATES: protein motif:HMM:NF024642.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNE06507.1"
                     /translation="KASLATEEKTRTAAQMMLLGDVQEALGEKENALLQNFLRGLNLP
                     GDQHVETLVVFPNIHDKQVQASRLRREIGEAQWAGREILQPEAIWSDFFSTSPMEALW
                     LEKLRQKFTPEVVVPADLTVRPIVERRREAGLTDYLLDYDQERAVKNELELPQDDQAL
                     PSEMRLNIINGVAGSGKTLILLYRLRLLYHLYPDKRFLVLTHNRPISHALQGNFARLT
                     RKEENTIEWRTFNGWCYHHWMKTPKWIEPLSLNARENIIGEIWRKYLQSTKISRKMFQ
                     SELDWFKDQLPMSRADYLVVDRRGRGFGLSEEMRQQMFDSMVGYQNQLRSRSALDWGD
                     IPRLLWKSTEDGSHQLPLYDFILVDEAQFFAPLWMRLIQKALVPRSGHLFMVADPTQG
                     FLGRGASWRSLGLDARGHTHHLRKSYRTTREIMQFATLL"
BASE COUNT          337 a          312 c          345 g          300 t
ORIGIN      
        1 aaggcgagtc tggcaaccga ggaaaagacc cgcaccgccg cccagatgat gttgctgggc
       61 gatgtacagg aagcattggg ggagaaggaa aacgcacttt tgcagaactt cctgcgaggc
      121 ttgaatttac ccggggatca gcatgtggaa actctggtcg tcttcccgaa tattcacgac
      181 aaacaagtgc aagccagtcg cttgaggcgg gaaattggag aggcgcagtg ggcgggcagg
      241 gaaatcttac agcccgaagc aatatggagc gatttctttt ctacatcacc aatggaagct
      301 ctttggttgg aaaagctgcg ccagaaattc acaccagaag tagtggtgcc agccgatctg
      361 actgtacgtc ccatagtaga acgccgacgg gaagcaggac tgaccgatta cttgctcgat
      421 tacgatcaag aacgggcggt caagaatgaa ttggaacttc cgcaagatga tcaggcactg
      481 ccatctgaaa tgcgtttgaa catcatcaac ggggttgcgg gcagcggcaa aaccttaatc
      541 ctgctatacc gtttgcgttt actgtaccat ctatatccag ataagcgctt tctggtgtta
      601 acacacaacc gcccgatcag ccatgctttg cagggaaatt tcgcccggct aacaagaaaa
      661 gaagaaaata ccattgagtg gcgaaccttc aatggttggt gctatcacca ttggatgaag
      721 acgccaaaat ggatcgaacc gctttctttg aatgcacgcg aaaacatcat tggtgaaatt
      781 tggcgaaagt atttgcaatc caccaagatc tcacgcaaga tgttccaaag cgagttggat
      841 tggttcaaag atcagcttcc catgtctcgt gcggattatc ttgtggtgga ccggcgtgga
      901 cgcgggtttg ggttgagcga ggaaatgcgt cagcagatgt tcgactcgat ggtcggatat
      961 caaaatcaac ttaggagtcg aagcgcattg gattggggtg acatcccccg cttattatgg
     1021 aaatccaccg aagatggttc ccaccaattg ccactttatg atttcatcct ggtcgacgaa
     1081 gcacaattct ttgcaccact ctggatgcgc ctgatccaga aagccctggt tccgcgcagt
     1141 ggacatttat tcatggtagc tgaccccact cagggatttt tgggacgcgg tgcttcatgg
     1201 cggtctctgg gtctggacgc gcgcggccat acccatcacc tgcgcaagag ttaccgcaca
     1261 acccgtgaga tcatgcagtt tgcgacctta cttt
//
LOCUS       DAOQJE010000968         1278 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_112238_length_1278_cov_6.069501, whole genome shotgun
            sequence.
ACCESSION   DAOQJE010000968 DAOQJE010000000
VERSION     DAOQJE010000968.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1278)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1278)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1278
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_112238_length_1278_cov_6.069501"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..193
                     /locus_tag="PLT08_18410"
     CDS             <1..193
                     /locus_tag="PLT08_18410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE06508.1"
                     /translation="VHLTLGILRLSQAVSYALAFFWSDGFAVPAPAQVTQTVSPLRES
                     TQILRNNIGGNSSTQNPDF"
     gene            159..>1278
                     /locus_tag="PLT08_18415"
     CDS             159..>1278
                     /locus_tag="PLT08_18415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE06509.1"
                     /translation="MEIHQLKTLISNLDSSDSSVVDETKKTLIEHADNIEVLNEFLNT
                     LHYGNQKIRENLVDVLSDIYSEQAFKLLMYAAHDTYHQVSNRVFDNLKKNSDPNEILF
                     FIEATLSYKEGPKPIAQEQALSWIREHKQTERRKYFLPLIKDYLSENHNIDLAIRGKL
                     LSVLAHHDYLDETEDLLDVVRHGDWRVKDYAINKLDKIINTDNRKEIYFALSEALQHE
                     INPETRSLLEKVLHPEFDKKTVFQSIKSSSDWFAEAVLYFRKLGFFQEYNTLADDELI
                     VKIKELRNYTPEDKFTYNTDLLLILAQDKNRVWYCDSETYWEQQYVKLFKEWGNISCG
                     KFTPENIIESRSDDNTDIHIDFSHNGEQTKIIATYYGDW"
BASE COUNT          436 a          241 c          245 g          356 t
ORIGIN      
        1 cgtgcacctg acgctgggga ttctgcggtt gtcccaagca gtttcctacg ccttagcatt
       61 tttctggtcg gacggcttcg ccgtccccgc cccagcgcag gtaacgcaaa ccgttagccc
      121 gctccgcgaa agtacacaaa tattgaggaa taatattggt ggaaattcat caactcaaaa
      181 ccctgatttc taatttggac agttcagact cttctgttgt tgacgaaaca aaaaagacct
      241 taattgagca cgctgacaac atagaggttt tgaatgaatt tttaaatacc ttacattatg
      301 gcaatcaaaa aataagagaa aaccttgttg atgttctttc tgatatctat tcagaacagg
      361 cttttaagtt actcatgtac gccgcacacg atacatatca tcaagtaagt aaccgtgtat
      421 ttgacaatct gaagaaaaac agcgacccga acgagatttt attttttatc gaagcgaccc
      481 tatcttacaa agaaggtcca aaaccaatag ctcaggaaca ggcattgtcg tggattcgtg
      541 aacacaagca aacagaaaga agaaaatatt tcctgccact aataaaggat tacctgagcg
      601 aaaaccataa tatagatttg gctattcgcg ggaaattact gtcagttctt gctcatcatg
      661 actatttaga tgagacagag gatctattag atgtggtgag gcatggagat tggcgcgtaa
      721 aagattatgc aataaataaa ttggacaaaa tcattaatac agataatcga aaggaaattt
      781 attttgctct atctgaggca cttcaacatg aaatcaatcc tgaaacgcgt tcattgctgg
      841 aaaaagtctt gcatcctgaa tttgacaaaa aaaccgtttt tcaatccata aaatcttcct
      901 cagattggtt tgcagaagca gttttatatt ttcgcaaact tggttttttc caagaataca
      961 atactttggc tgatgatgag ttaattgtaa aaataaagga gctgcgtaat tacacacctg
     1021 aagataagtt tacatacaac accgacttat tgcttatact tgcacaagat aagaatagag
     1081 tatggtactg cgactctgaa acgtattggg aacaacaata tgtaaaactg ttcaaggaat
     1141 ggggcaatat atcttgcgga aagttcacac cagaaaatat tattgaatcc agaagtgatg
     1201 acaacactga cattcatatt gatttcagcc ataatgggga acaaacaaaa attattgcaa
     1261 cttactatgg cgattgga
//
LOCUS       DAOQJE010000969         1276 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_112629_length_1276_cov_3.822277, whole genome shotgun
            sequence.
ACCESSION   DAOQJE010000969 DAOQJE010000000
VERSION     DAOQJE010000969.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1276)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1276)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1276
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_112629_length_1276_cov_3.822277"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(520..1149)
                     /locus_tag="PLT08_18420"
     CDS             complement(520..1149)
                     /locus_tag="PLT08_18420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE06510.1"
                     /translation="MTATKKKPKRHEEENTTSKTEKNNASIWVAIITGVVSITLGILG
                     FPPLVTYLNAQWNNTPTPQPTEAIFLVPSETPVFSILTIATPQETNIVTPTITTTPTS
                     TFEPISGVMNAQISYNYSTGNAPLNVSFNAHASYVSYPDGQKVECQFENVCTYTWDVR
                     TKEGKTISSPTGGPAEFSYTFSKKGEYMVVVYVCRGDVCNFTATNIVVK"
BASE COUNT          332 a          266 c          298 g          380 t
ORIGIN      
        1 tttcgagaac cggctctaat aaatctgcca cccccatggt tgcggtcact tcaatgttgg
       61 ggtctttgcc aagtttggca agataccctt ccacggtcat ggggtgatcg tccaggatgg
      121 caacgcgtat ttttgattgc atgttcaccg ctcccactgc atcgtccttc tctcaaagat
      181 tgttgactat tgctcattaa taaaaagggt aacagaaaga gaattctgtt tcaagagata
      241 cagtacaaat gcctgccttc tggttattga ctgtacttgt agagtctata aaataaaaaa
      301 ggtttggaat acgtttggtg tcttccaaat ccatagaaca tggggggaac agggtgcgca
      361 ccagcctctg ggcggctcga acgtcagtgg actgacggag gaggctgggc gtgccatagg
      421 ggatgccttt cttctttgct tgctggcgca tcggcgcaag gggaggggac acccgatgcg
      481 aaacaacggg gcgcacgcca ttgggggagg cgtgcggttt tatttaacaa caatatttgt
      541 ggcagtaaaa ttacatacat ctccccggca gacataaacc accaccatat attccccttt
      601 tttgctaaaa gtataggaaa actcagccgg tcctccagtg ggacttgaga ttgttttccc
      661 ttcttttgtg cgaacatccc atgtgtatgt gcaaacattt tcgaattggc attctacttt
      721 ttgtccatcc ggataactta cataagaagc atgtgcatta aaagaaacgt tcaagggcgc
      781 atttccagtg ctatagttat aagaaatttg agcattcatt accccactga ttggctcaaa
      841 agtagatgta ggggtggtag ttatagtagg agtgactata tttgtttctt ggggtgttgc
      901 tattgtaagg atagaaaaaa caggtgtttc acttgggacc agaaatatgg cttcggtagg
      961 ttgaggcgtg ggcgtattat tccattgggc gtttagatac gtaaccaatg gaggaaaccc
     1021 aagaattccc aaggttattg agacaacccc agtaataata gcgacccaaa tactggcatt
     1081 atttttttca gtttttgagg ttgtattttc ttcttcatgc cttttcggtt tctttttagt
     1141 agctgtcatg acatcctccc gatatttatg tgcgatgtta atgctcattt taagaattcc
     1201 aaaaggaaag tcaaggcact gctttctcta acaatcctcc aatattcccc atgctataat
     1261 cccttcctcg gaaggg
//
LOCUS       DAOQJE010000970         1265 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_114640_length_1265_cov_2.530579, whole genome shotgun
            sequence.
ACCESSION   DAOQJE010000970 DAOQJE010000000
VERSION     DAOQJE010000970.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1265)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1265)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1265
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_114640_length_1265_cov_2.530579"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..677)
                     /locus_tag="PLT08_18425"
     CDS             complement(<1..677)
                     /locus_tag="PLT08_18425"
                     /inference="COORDINATES: protein motif:HMM:NF018178.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1080 domain-containing protein"
                     /protein_id="HNE06511.1"
                     /translation="MGDKKDDTEDKTSVARWSFYATIIASLIGGIISLIVADKIPSFR
                     PSTPTPEVLVINPGTILLDENFEDRQMQGMSINGDWKIIADETKNQVINSDSPNGGNF
                     IGFGSEAWGNYLFEFRIRFLSTNNQGIGLEFRRTCDNAQNCFRYVLILSRDSINLYFD
                     SPESPYTPIQASSYDFNTQSWQDVRIEAKNSNIKIFINNSLTIEANDSNLAKGNLLFG
                     VENNHVQL"
     gene            complement(843..>1265)
                     /locus_tag="PLT08_18430"
     CDS             complement(843..>1265)
                     /locus_tag="PLT08_18430"
                     /inference="COORDINATES: protein motif:HMM:NF040295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6000 family protein"
                     /protein_id="HNE06512.1"
                     /translation="EWSGWREQITGSWFCGIKGWSQFSDLIGKELVESKMTYAGQGLS
                     FALACFANKKSIHYLMEYLDMYLPQTNLVYDQDWVMSALLWVDEQNKTNYASRYLVPG
                     GLWEKYVSGKSVELSKCKENLWNLMKYCQEHFVKPKAG"
BASE COUNT          372 a          264 c          217 g          412 t
ORIGIN      
        1 agttgaacat gattgttctc aactccaaag agaaggttcc cttttgcaag attagagtca
       61 tttgcttcga ttgtcagcga attattaata aaaattttta tattgctatt ttttgcctca
      121 attctcacat cttgccaaga ttgtgtattg aaatcatacg aacttgcttg gattggtgtg
      181 tagggcgatt ccgggctgtc aaaatataaa ttaattgagt ctctacttaa gattaataca
      241 tatctaaagc aattttgggc gttatcgcaa gtgcgtctaa attcgagacc aattccttga
      301 ttgttagtac ttaaaaacct tatccgaaat tcgaataaat aattacccca agcctcagag
      361 ccaaaaccta taaaattgcc accattaggg ctatcggagt tgattacttg attttttgtt
      421 tcgtctgcaa ttatcttcca gtcaccatta atagacattc cttgcatttg acgatcttca
      481 aagttttcgt ctaataaaat ggttccagga ttaattacta aaacttcagg cgtaggtgtc
      541 gatgggcgaa aagaaggaat cttgtcagca actatcaatg aaattattcc tcctattaat
      601 gaggcaataa tagttgcgta aaatgaccaa cgtgcaaccg aggttttatc ttccgtatcg
      661 tcttttttat ctcccatttg atattatcct tttttgttag aaataattct acatagccac
      721 cgaacggttt gcgttacctg cgctggggtg gggacggcga agccgtccaa ctagaaaaat
      781 gataaggcgt gtaaaaatgc ttgagattgc cgcagactcc ccagcgtcag gtgcacgctt
      841 tgttagcccg cttttggctt tacaaaatgt tcttggcaat atttcatcaa attccataaa
      901 ttttctttgc attttgacaa ttcaacactt ttccctgaaa catacttctc ccataagcca
      961 ccaggcacaa ggtagcggct agcgtaattt gtcttatttt gttcgtcaac ccataataat
     1021 gctgacatca cccaatcttg gtcgtaaacc agatttgttt gtggaagata catatccaaa
     1081 tattccataa gatagtgtat gcttttttta tttgcaaaac aagcaagagc aaaactgagt
     1141 ccctgaccag cataagtcat tttgctttct acaagttctt tgccaataag atcagaaaat
     1201 tgcgaccaac ctttaattcc gcaaaaccaa ctcccagtta tttgctctcg ccaaccactc
     1261 cattc
//
LOCUS       DAOQJE010000971         1265 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_114685_length_1265_cov_1.975207, whole genome shotgun
            sequence.
ACCESSION   DAOQJE010000971 DAOQJE010000000
VERSION     DAOQJE010000971.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1265)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1265)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1265
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_114685_length_1265_cov_1.975207"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..657
                     /locus_tag="PLT08_18435"
     CDS             <1..657
                     /locus_tag="PLT08_18435"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013704.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HNE06513.1"
                     /translation="VTDPPYCSGGRQQNGARNQIEKSERAPDEWFLADNMGTDTYIRW
                     MRIIAKETLRVSAMGAHAYIFTDWRQYTNVVTAWESIGWTLRNVIVWDKAFGGAMGSF
                     WRNNHEWVCVFAKGAPSALPDGSFYNTWKGTKPQGGHHPTEKPIKFLEYIVRAVDGIV
                     LDPFMGSGTTGVACAKMNRGFIGIERDAQYFDIAKGRIASAYGEHRESNLPLFASNAT
                     "
     gene            837..>1265
                     /locus_tag="PLT08_18440"
     CDS             837..>1265
                     /locus_tag="PLT08_18440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE06514.1"
                     /translation="MKSLFKKSSSFPLNLLLILALASGLVSCSQQSVAASSAQSEQLP
                     VATQTAESQPTEQFGNFENSYGITVTAKALCTDGANTTLYLETNLDSSLWQLSKKDFY
                     PSGKTYFETSILFLENDEMFSMYSSGKRDDPVFNSQDSIVS"
BASE COUNT          363 a          268 c          305 g          329 t
ORIGIN      
        1 gtcacagacc cgccatattg ttcaggcggc aggcaacaaa acggcgcaag aaatcaaatt
       61 gaaaaaagtg aacgcgctcc cgatgagtgg tttttagccg ataacatggg aacggatact
      121 tatatccgat ggatgagaat tatcgcaaag gaaactttga gagtgtcagc aatgggcgca
      181 catgcttata tttttactga ctggcggcaa tatacaaatg tcgtcacagc atgggaatca
      241 atcggctgga ctttgcggaa tgtcatcgta tgggataaag ccttcggcgg cgctatgggt
      301 tcgttctggc gcaacaatca cgaatgggtt tgtgtgtttg ctaaaggtgc gccgtccgct
      361 ttgcctgatg gttcttttta taacacttgg aaaggcacga agccgcaagg tggacaccat
      421 cctacggaaa agcctatcaa gtttttagaa tacattgttc gcgcagttga tggaattgtg
      481 cttgacccat tcatgggtag cggcacgaca ggcgtagcct gtgcaaaaat gaatcgtggt
      541 tttattggca ttgaaagaga cgcgcaatat tttgatattg ccaaaggcag aatagcatcc
      601 gcctatggtg agcatcgtga aagcaatttg ccgctctttg ccagtaacgc cacctaacaa
      661 agcgtgcagc cgacgtgtgg gattcggcgg catttacagg gctgatacta gccccagcag
      721 tttactaatc tcaaagtgtt tgctacgccc gcccacacgc ggctaacgca aaccgttggg
      781 cggttggtga taatggctca agtttcattc ggacaaatct aattcgtgga gaaaaaatga
      841 aatcattatt caaaaagagt tcaagttttc ccctaaatct cttgttgata ttggctcttg
      901 caagcgggct tgtttcctgt tcacagcaat cagtggcagc gagttcagcc caaagcgagc
      961 aattaccagt tgccacccaa acggcagaga gtcaaccaac tgaacaattt ggaaattttg
     1021 aaaactcata tggcatcacg gttacagcaa aagcattatg caccgatggc gcaaacacta
     1081 cactttattt agaaactaat cttgattcga gtctttggca attgagcaag aaagacttct
     1141 atccttctgg gaaaacttac ttcgagacaa gtattttatt cttggaaaac gatgagatgt
     1201 tttcaatgta ttcaagcgga aagcgggacg acccagtttt taattcgcaa gatagtatag
     1261 ttagc
//
LOCUS       DAOQJE010000972         1262 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_115120_length_1262_cov_3.710853, whole genome shotgun
            sequence.
ACCESSION   DAOQJE010000972 DAOQJE010000000
VERSION     DAOQJE010000972.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1262)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1262)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1262
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_115120_length_1262_cov_3.710853"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            165..1133
                     /locus_tag="PLT08_18445"
     CDS             165..1133
                     /locus_tag="PLT08_18445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE06515.1"
                     /translation="MKTFHKACQIIFQIAIINFLLFVFIAIMIGGDAVNGHAENGHYY
                     LANHGQLTEVNYFVFMYSKLHAYSIFITHPLAMLAGFLYWITGGGSVRPKSLAAYPHS
                     KSSLLSIIKSFLWKVADLIEGVFWIILDSWRKPDYELFVRLSKEECIRELQAGTDNEP
                     TLYKLNKPLWGYFSGNHFYLQKWNYNPYFRDGGVRPVLAGKFSSTPQGTYIRLWHRFT
                     TIGIFFLTTWFGTALSLLSLYLISTNIHSSQQYAYLDSRLPMLALIIAPILYISTLFI
                     SIQIGSIFGKSNNFDIIEFVKDLLDHHHTSSQLAGFRLSRKMQGKV"
BASE COUNT          357 a          278 c          240 g          387 t
ORIGIN      
        1 ttaaaagcgc ccaacaaagc gtgcacctga cgctggggcg cttcgcgtgc gcacatccca
       61 ggcagttttc tatgccctag cctttttctg attggacggc ttcgccgtcc ccaccccagc
      121 gcaggtaacg caaaccgttg ggtggcttct ctgcaaaatt atcaatgaaa acatttcata
      181 aagcatgtca aatcatattt caaatagcaa taattaattt tttgctgttt gtatttattg
      241 caattatgat tggcggcgac gcagttaacg gtcatgctga aaacggacat tattatcttg
      301 ccaatcatgg acaacttaca gaagtaaatt attttgtctt tatgtacagc aagttgcacg
      361 cttacagcat ctttatcact cacccattgg ctatgttggc aggattttta tactggatta
      421 caggtggtgg gagtgttcgt ccaaaatcac tggctgcata tccacactcc aagagcagtt
      481 tgctttcaat tatcaaatct ttcttatgga aagtagcgga tttaattgaa ggtgtcttct
      541 ggataatttt ggattcctgg agaaaaccag attatgagct gtttgttcga ctttccaaag
      601 aagaatgtat cagagaacta caggctggaa ccgataatga acccacactt tataaactaa
      661 acaagccact ttgggggtat ttcagcggaa accattttta tttacaaaag tggaactaca
      721 atccgtattt cagagatggg ggtgttcgcc cagttctggc aggtaaattt tcatctacac
      781 cacaaggcac atacatcagg ctttggcata gatttacaac aattggaata ttctttctta
      841 caacttggtt tggaaccgct ttaagccttt tatctttata tcttatctca accaatatac
      901 attcaagcca gcagtatgca tatttagatt ctagactccc gatgctcgct ttaattattg
      961 cacccatact ttatataagt accttgttca tttctattca aattggctca attttcggga
     1021 aatcgaacaa cttcgatata attgaattcg taaaagacct gcttgaccat catcatactt
     1081 ccagtcaatt agcaggattt agattgtcac gaaaaatgca aggtaaagtt taattaaaac
     1141 gcaacccaac aaagcgtgca cctgacgctg gggattctgc gcacatctca agcatttttc
     1201 tacgccttag cttttttctg gttggacggc ttcgccgtcc ccgccccagc gcaggtaacg
     1261 ca
//
LOCUS       DAOQJE010000973         1261 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_115337_length_1261_cov_2.927861, whole genome shotgun
            sequence.
ACCESSION   DAOQJE010000973 DAOQJE010000000
VERSION     DAOQJE010000973.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1261)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1261)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1261
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_115337_length_1261_cov_2.927861"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(72..1133)
                     /locus_tag="PLT08_18450"
     CDS             complement(72..1133)
                     /locus_tag="PLT08_18450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="WD40 repeat domain-containing protein"
                     /protein_id="HNE06516.1"
                     /translation="MMFQTKVFRLSIAALLILVFLSGCNHSVTPYLLAEWKIGNLKDM
                     VWSPDSETFVVNYWIDNNEANNFVQAFSLKTLNSIWIAENSLAWDVVFTPDGKFIVES
                     NTNIPTFYWRSIEDGRIIRKDERSENNKGCNGGGQVLIVNSQENTALLVNYGDLLGPS
                     WNTHNIVVLRKYDFETGKCTDLFNYQGTFDLIELNSNGTLMAYGGEGKDDSVVIFDME
                     KQIELCRIPQVEFGRFVPNENTLAVVREERMVFINAQTCKEIKELNVSPKSDYENYFA
                     FNPNGKQFAIARDSIEIRDTGTGEIIAQIPFPDKAVPNSSKLFLSGIKFSPDGKYLLI
                     SYFPLDSVYSGQIQLWQLK"
BASE COUNT          376 a          267 c          228 g          390 t
ORIGIN      
        1 ttgagactgt gcgcacgcga agcgccccag cgtcaggtgc acgctttgtt gggcgttttt
       61 tgactttcga ttcattttaa ttgccaaagt tgaatttgac cactgtaaac actgtccagg
      121 ggaaaataag aaattaagag atactttcca tcagggctaa acttaattcc acttaaaaat
      181 aacttacttg aattaggaac tgctttatca ggaaaaggga tttgcgcgat tatttcacct
      241 gtaccagtat ccctgatttc aattgagtct cgtgcaatgg caaattgctt tccgttcgga
      301 ttgaaggcaa aataattttc gtaatcagat ttgggggata cgtttaactc ttttatctct
      361 ttgcacgttt gagcattaat aaaaaccatt ctttcttctc tgacaactgc taaagtattt
      421 tcatttggaa caaagcgccc aaactctact tgggggatac gacataactc tatttgtttt
      481 tccatatcga atataacgac agagtcatct tttccttcgc caccgtaagc cataagagta
      541 ccgttggaat tcaattcaat caaatcaaat gttccctgat aattgaataa atcagtacat
      601 ttccctgttt caaaatcgta ttttctaagt acaacaatat tgtgtgtatt ccaacttggt
      661 ccaagtaggt cgccataatt tacaagcaag gctgtatttt cttgagaatt aactatgagg
      721 acttgcccgc caccgttaca acccttattg ttttctgaac gttcatcttt tcgtattatt
      781 ctgccatctt ctatacttct ccaatagaag gtgggaatat ttgtgtttga ctctactata
      841 aactttccat ctggagtaaa aactacgtcc catgcgagac tattttcagc aatccaaata
      901 ctattgagcg ttttcagaga aaatgcttgt acgaaattgt ttgcttcatt attatctatc
      961 caataattaa ccacaaacgt ttcactgtca ggactccaaa ccatatcttt caaattgccg
     1021 attttccact cagcaagcaa atatggagta acagaatgat tacagccgct caaaaacacc
     1081 aatataagca aggctgcgat agataggcga aatactttcg tttgaaacat cataaattca
     1141 tgctatgcaa gggaacgccc aacggcttgc gttacctgcg ctggggcggg gacggcgaag
     1201 ccgtcaagcc agaaaaatgc taaggcgtag aaaactgctt gagatttgcg cagaatcccc
     1261 a
//
LOCUS       DAOQJE010000974         1255 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_116402_length_1255_cov_4.240000, whole genome shotgun
            sequence.
ACCESSION   DAOQJE010000974 DAOQJE010000000
VERSION     DAOQJE010000974.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1255)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1255)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1255
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_116402_length_1255_cov_4.240000"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..248
                     /locus_tag="PLT08_18455"
     CDS             <1..248
                     /locus_tag="PLT08_18455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE06517.1"
                     /translation="RCTTMDAPEAAAKVSFSEWAVNIRMPYRNETFDTIGTTGNLATV
                     RVMVELKIDGKWTEKQTEVHCEKVNATWECDRAMKFK"
     gene            390..1175
                     /locus_tag="PLT08_18460"
     CDS             390..1175
                     /locus_tag="PLT08_18460"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HNE06518.1"
                     /translation="MLLKDRTAIVYGASGAVGSAVALAFAREGARVILSARRREPLEK
                     IASEITSLGGLAEVMTVDAMDIHAIESHLEAVSTRFGAVTLMFNAIPWDDVQGQALAE
                     MPFDQFMSPIRNGLTTWFHTGTVLAKHMSNHGGGTILGITANAGRQPFANVGGFGIAC
                     AAVEHYLRQLAVENGPSQVRVCWVRSPGSPDAPGVREAWEMYGEQKGMTFEEVHREFS
                     KDVPLRRITPLAQVANAAVLLASDLAAGMTATMANATGGSQVD"
BASE COUNT          339 a          287 c          346 g          283 t
ORIGIN      
        1 ctcgatgcac tacgatggat gctccagaag cagcggcaaa agtcagcttc tctgaatggg
       61 cagtgaatat tcgaatgccc taccggaatg agaccttcga cacgatcggt acgactggta
      121 atctggcaac ggtacgtgtt atggttgaat tgaagatcga tgggaaatgg actgagaagc
      181 agacagaggt ccactgcgaa aaggtcaacg ctacatggga atgtgatcga gcaatgaaat
      241 tcaaataaca tatccaatag caattacaag gcattgggga atgtataaaa tcttatacgg
      301 gtatactagc cgtcaaatac gcgagagcga gcgggattta tcggtcaaac cgcacataca
      361 gttatgcatc acctttttag gagcaaacca tgttacttaa agatagaaca gcaattgttt
      421 acggagcatc cggtgcggtt ggaagtgcgg tggcgcttgc cttcgcacgt gagggcgcaa
      481 gggttatttt gtcagcgcgt cggcgtgagc cgcttgaaaa aatagccagc gaaatcacaa
      541 gcctgggtgg gctggcagaa gtaatgaccg ttgatgcgat ggatatccac gcgatagaga
      601 gccatttgga agccgtatcc actcgttttg gtgccgtgac gttaatgttc aatgcgatcc
      661 catgggatga tgttcaggga caggcactcg ctgaaatgcc cttcgaccag tttatgtccc
      721 ccatacgaaa cggattgacc acttggttcc atacaggaac cgttctggca aaacacatgt
      781 caaaccacgg aggcggcacc attctgggga taactgccaa tgccggtcgg caaccctttg
      841 cgaatgtggg tggcttcggt attgcatgtg ccgccgttga acattatctt aggcaattag
      901 ccgttgaaaa cggtcccagc caagtgcgag tgtgttgggt ccgttcaccg gggtcacccg
      961 atgcacccgg cgttcgtgaa gcatgggaga tgtatggaga acaaaagggg atgacctttg
     1021 aagaggttca tcgtgaattt tcgaaagacg tccccttgcg acgcatcact ccattagctc
     1081 aagtggcaaa cgcagccgtc ctcttggcga gtgaccttgc cgccggaatg accgccacca
     1141 tggcaaatgc aactggcggg tcacaagtag actaattcgc gctcatgaga tcgcatttta
     1201 tgagaaagga aaaaagcgga aagaatccgt ctattggcag gcacgttcaa tgtcc
//
LOCUS       DAOQJE010000975         1255 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_116516_length_1255_cov_1.931667, whole genome shotgun
            sequence.
ACCESSION   DAOQJE010000975 DAOQJE010000000
VERSION     DAOQJE010000975.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1255)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1255)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1255
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_116516_length_1255_cov_1.931667"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..759)
                     /locus_tag="PLT08_18465"
     CDS             complement(<1..759)
                     /locus_tag="PLT08_18465"
                     /inference="COORDINATES: protein motif:HMM:NF017822.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DpnI domain-containing protein"
                     /protein_id="HNE06519.1"
                     /translation="MKLRLTSEIASNYSSASQKIRVLTESWVGNSIFCPNCGSALNHF
                     ENNSPVADFYCKKCAEEYELKSKNGEMGKKIVDGAYSTMIERLKSDNNPNFFFLTYDK
                     STFEIRNFLTIPKYFFVPAIIEKRKALSHTARRAGWIGCNIDVSKIPELGKIFFVQDG
                     IVRSKDEVLEKWSKTYFVKATQSIETKGWLLDVLMCVEKIKKEEFSLDDVYPFEGYLK
                     AKHPSNNNVKAKIRQQLQFLRDKGVLDFVGRGQYR"
BASE COUNT          360 a          308 c          193 g          394 t
ORIGIN      
        1 ccgatattga ccacgaccga caaaatcaag aacgccttta tcccgtaaaa actgtaattg
       61 ctggcgaatc tttgccttca cattgttatt ggatggatgt ttggctttca aatagccttc
      121 aaaaggataa acatcatcta gtgaaaactc ttcctttttg attttctcca cacacatcaa
      181 aacatccaat agccagcctt ttgtttcaat gctctgagtt gcttttacaa aataagtttt
      241 gctccatttt tcaagtactt catctttact tctgacaata ccatcctgaa cgaaaaagat
      301 tttccccaat tcggggattt tgctgacatc gatattgcaa ccaatccaac ctgctcgtct
      361 tgctgtatga gaaagtgctt ttctcttttc aataattgca ggaacaaaaa agtattttgg
      421 gatagttaaa aagtttctaa tttcaaaagt ggatttgtcg taagtcaaaa agaaaaagtt
      481 gggattgtta tctgatttca ggcgctcaat catcgtggag tatgcgccat ctacaatctt
      541 ctttcccatt tccccattct tagactttag ttcgtattct tcagcacact tcttacaata
      601 aaaatctgca acaggcgaat tattttcgaa atgatttaat gcacttccgc aattgggaca
      661 aaaaatcgaa ttaccaaccc aactttccgt caatacgcgg attttttgag aggcacttga
      721 ataattgctt gcgatttctg aagtcagtct tagtttcata tttttcagtc actgattgga
      781 ttaagataca ttttcaccca ttcatccaac ggagtcaaat attcatatcc caaaacacgg
      841 acatcgtgcc agcaatcatc acacaattcg agtaaatctg tcgagccttc ttcccagcaa
      901 atctttttat ggcatcgttc acactcccaa agttcacctt ggcatgaagt gtgatttttg
      961 ctttcgcaac cgacaatcct attgcctctc ctctttatac tcatggcatg gctattataa
     1021 aggaccgaaa aaaagaggtt gtgtccaaaa atagacacaa cctcttttca caaatagact
     1081 gattttttgt ttttacttcg tgggtgtaac ccctacccac caaaccccaa ctccttcaaa
     1141 ttctcctcgc gagatttctt ccccccgctc atctccttcc aaaacgcctc atcatacctt
     1201 cgtgaccgca caggcagcgg cattggcaca ccccaaccga gcagcagcac ctctt
//
LOCUS       DAOQJE010000976         1250 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_117369_length_1250_cov_6.756485, whole genome shotgun
            sequence.
ACCESSION   DAOQJE010000976 DAOQJE010000000
VERSION     DAOQJE010000976.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1250)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1250)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1250
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_117369_length_1250_cov_6.756485"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(41..775)
                     /locus_tag="PLT08_18470"
     CDS             complement(41..775)
                     /locus_tag="PLT08_18470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE06520.1"
                     /translation="MVSNFKQGTHSVSGIDLVIHDLLDGDSGYIEDYLRIYQELFPQY
                     VRYLPVMRRRAEERADHTSIEKWHQWLLIVNDVPVGIVGFLYNRKKNVGLLMDFAIYP
                     DARRLRGVDGSPFSKVILGLAMDQLVEDARLEAQTAPICLAAEVEYPRLLQKYMEYGY
                     VKFPVEYFEPPSTPELEKVGDKIQELKNAEFGKMNIGAFQIPGHPNDLSDTNIIKIVL
                     LAFLEDHYQLPLDHWLIRKVIQEISA"
     gene            complement(759..>1250)
                     /locus_tag="PLT08_18475"
     CDS             complement(759..>1250)
                     /locus_tag="PLT08_18475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE06521.1"
                     /translation="VFQWLITSRVLSKIHLKEAFAVFPVSLAIASGVALSISSVFGAA
                     SARFVARTVYAGWDDPARKSLQGLVPDEKRGRISAFMDSYFITTATVIGCIVLIILLT
                     LVSNSIITSALAITIYLSIALVAALLGIIAFIYLRRVYDTSMLNYRLARSKRKSVLDG
                     IEF"
BASE COUNT          399 a          285 c          235 g          331 t
ORIGIN      
        1 caggtaatgt atctactttt tcagtcatgc gatctatctc ctatgcagaa atctcctgaa
       61 tcacttttcg tataagccaa tgatccaacg gtaattgata atgatcttcc aaaaatgcca
      121 gcaacactat tttaataata ttggtatcgc taagatcgtt agggtgtccc ggtatctgga
      181 atgccccaat attcattttt ccaaactcgg cgttcttaag ttcttgaatt ttatcaccaa
      241 ctttttccaa ttcaggggta gaaggcggct caaaatactc gacagggaac tttacatacc
      301 catattccat gtatttttgc aacaaacgtg gatattccac ctcagctgcc aggcaaatag
      361 gagcagtttg cgcttccaat ctggcatctt caacaagttg atccatcgca agccccaaaa
      421 tcaccttaga aaaaggcgat ccatctacgc ctctaagcct tcttgcatct ggataaatgg
      481 caaagtccat taataatcca acattctttt tccggttata caaaaagcca acaatcccaa
      541 ccggcacatc attgacaatt aacagccatt gatgccattt ttcaatggaa gtatggtctg
      601 cccgctcttc agcacggcgc cgcattacag gtaaatatcg tacgtattga gggaaaagct
      661 cctgataaat tcttaggtag tcttcaatat agccagagtc tccatcaagc aaatcgtgga
      721 tgactaagtc aataccagag acactatgcg tgccctgttt aaaattcgat accatcaaga
      781 acacttttac gttttgaacg cgcaaggcga taattcaaca tgcttgtatc gtagaccctg
      841 cgaaggtata taaatgcaat aattcctaac aaagcagcga caagagcaat tgaaaggtag
      901 attgtgattg ctaatgcaga tgtaattatg ctattcgaca caagggttaa aagaatgatc
      961 agaacaatac aaccaatcac agttgcggta gttataaaat aactgtccat aaaagcagaa
     1021 atgcgaccgc gtttttcgtc tgggacaagt ccttgcagcg attttcgtgc agggtcatcc
     1081 caaccagcat atacggtacg agcaacaaaa cgagcggacg ctgccccaaa tacggaagag
     1141 atagataaag ccaccccact agcgattgcc aaggaaactg gaaaaacagc aaatgcttct
     1201 tttaagtgga tcttcgataa aacgcggctc gtaattagcc attggaatac
//
LOCUS       DAOQJE010000977         1248 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_117743_length_1248_cov_6.730930, whole genome shotgun
            sequence.
ACCESSION   DAOQJE010000977 DAOQJE010000000
VERSION     DAOQJE010000977.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1248)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1248)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1248
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_117743_length_1248_cov_6.730930"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            373..906
                     /locus_tag="PLT08_18480"
     CDS             373..906
                     /locus_tag="PLT08_18480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE06522.1"
                     /translation="MDDKYSFDRLGEYTALLAGYSWLEVETYLINNLEEGYNHDVFFS
                     ALLRDKLYQRSYQVPRVLDKGYEIHGPFYLSKLNSENFKKITIEQFSVKINDIFYSEV
                     GFTKNDEKIDIARQQEARWLLNKLPKDWADYYELNVSREDEKYHHESWFILTIFYEFI
                     IVERSTKKLYLCILGED"
BASE COUNT          365 a          252 c          289 g          342 t
ORIGIN      
        1 gtgcatattc cacccctttc gctttgcacc gataatgcag cgatgatcgg cgcggcggga
       61 tatttccgtt atgccctggg gcacctctcc gacctggaga tggacgtcct gccgacctgg
      121 ccgctttcat agtggccttg aatgaaccag ggattcttgg agcggagagc ctgatcccct
      181 gcgggttaga gatcaggctg taaaaaaaca aacgcattgg agaatggttt ctcttgcggg
      241 caacttggta agatcctaca aatggcggtt aagtaccccg ttttgggtgt ttatcccata
      301 gggacattac ggtgcgctgt ataaacagta gttgggctgc taagaacata gactcaaatg
      361 agcaatttaa atatggatga taaatatagt ttcgataggt taggagaata tacggcttta
      421 cttgctggtt atagttggtt ggaagtcgag acctatttaa tcaacaatct tgaagaaggt
      481 tacaatcacg atgtattctt cagtgcatta ttaagagata aactgtatca gcgctcctat
      541 caagtgccaa gggtgcttga taaaggatat gaaattcatg gaccttttta tctttcaaaa
      601 ctcaattcag aaaacttcaa gaaaattact attgaacaat tttctgtaaa aattaatgat
      661 attttctact cagaagttgg cttcacaaaa aatgacgaaa aaattgatat agccagacaa
      721 caggaagcaa gatggttgtt aaataaactt ccaaaagatt gggctgatta ctacgaactt
      781 aacgtcagtc gagaggatga aaaatatcac cacgagtctt ggttcatcct tacaatattt
      841 tatgaattta taatcgttga gcggtcaaca aaaaaacttt atttgtgtat tttaggcgaa
      901 gactaatctg caaaacgcag cccaacaaag cggccgcctt tgtcgtttaa aacaatcact
      961 ccccagtggc ttgcccaagc cagacagggg agtaatcagg agaatgtaat tcttattata
     1021 tggaggcagt ttaatgcttc ctgaacattg cttaagtatt ggatgagaga ttattcgagg
     1081 gcggagagca gttcttccag caagatcggc ccttcccgca gaatttttgg agattcccct
     1141 gtgcaatcca ccagcgtgga aggggtgcca cccggtgtct gaccgccatc taagatcaaa
     1201 gggatgcgtc cctgcaactg ttcgtagact gcttgcgcgg tcaccggg
//
LOCUS       DAOQJE010000978         1246 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_118149_length_1246_cov_2.704450, whole genome shotgun
            sequence.
ACCESSION   DAOQJE010000978 DAOQJE010000000
VERSION     DAOQJE010000978.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1246)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1246)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1246
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_118149_length_1246_cov_2.704450"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..252
                     /locus_tag="PLT08_18485"
     CDS             <1..252
                     /locus_tag="PLT08_18485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE06523.1"
                     /translation="PELIEEGFEPHMPKEVWCSLTNQPNTTVDVTEFWDVKLQAILQH
                     KTQVQDAEKLIERFKSRRAEDSTEDNPRYEEKFRVVKYS"
     gene            325..>1246
                     /locus_tag="PLT08_18490"
     CDS             325..>1246
                     /locus_tag="PLT08_18490"
                     /inference="COORDINATES: protein motif:HMM:TIGR01891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004180 - carboxypeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HNE06524.1"
                     /translation="MTNPFLKQAQELFPYTQAMRRDFHKHPELGYREVRTGGIVAKEL
                     EALGIEVTKGVGKTGVVGFLEGAKPGPTILLRFDMDALPITEDTGAEYTSENPGVMHA
                     CGHDGHTAIGLTVAKILNERKDELAGNIKFCFQPSEEGTNGEDIGGALMMMRDGVLEG
                     PKVEKTLSLHLWNYYPLGWVHIAQGPIMAGADLFQINLTGKGGHGAIPESAVDPIIAA
                     SQIVNALQSIVSRNVTPLKPAVVSVTSIHSGTAFNVIPQTAELKGTIRTFDREVRELV
                     HKRFYEIVNGIAVSMGCESEIMIKEVTAPVI"
BASE COUNT          344 a          327 c          300 g          275 t
ORIGIN      
        1 cccgagttga tcgaagaagg ctttgaaccg cacatgccca aggaagtttg gtgttcgctc
       61 accaaccagc ccaacaccac agtagatgtg actgaattct gggatgttaa gttgcaagcc
      121 attctgcaac ataagactca ggtgcaagat gcagaaaaat tgatcgagcg tttcaagtcc
      181 cgccgggctg aagacagcac agaagataac ccgcgctacg aagagaaatt cagagtcgtg
      241 aaatatagct agaactacag tcgaaagcgg aagcaacctt ccactttcga cttttgactt
      301 aacacccatt gaaagcagat aaacatgaca aaccccttcc tcaaacaagc ccaagaactt
      361 tttccctaca ctcaagccat gcgccgcgat tttcacaagc accccgagct tggctatcgt
      421 gaagtccgca cgggcggtat tgttgccaaa gaactcgaag ccttgggcat tgaagtcacc
      481 aaaggtgtcg ggaaaactgg cgtggtgggt tttcttgaag gcgccaagcc gggtcccacc
      541 attttgttgc gtttcgatat ggacgccttg cctatcaccg aagatacagg tgcagaatac
      601 acctccgaga atcccggcgt gatgcatgcc tgcgggcatg atggtcacac cgccattggg
      661 ctgaccgtag ctaagattct gaacgaacgc aaagacgaat tggctggcaa tatcaagttt
      721 tgttttcaac cttcagaaga aggcaccaat ggcgaagaca tcggcggcgc attaatgatg
      781 atgcgcgacg gcgtgctcga aggtcctaaa gtggagaaaa ctctctcgct tcatttgtgg
      841 aattattacc cgctcggttg ggtacacatc gctcaaggtc cgatcatggc aggcgcagac
      901 cttttccaaa tcaacctgac aggcaaaggc gggcatggcg ccattcccga atcagctgta
      961 gatccaatta ttgccgcctc acagatcgtc aacgctttgc agagtatcgt atctcgtaac
     1021 gtcacacctc taaaacctgc tgtggtttca gtgacctcga tccattcagg gacagcattc
     1081 aacgtcatcc cacaaacggc agaactcaaa ggcacgatcc gcacctttga tcgcgaagtc
     1141 cgcgagttgg tacataaacg tttctacgaa atcgtcaatg gcattgcggt ttccatggga
     1201 tgcgaatccg agatcatgat caaagaagtc actgcgcccg taatca
//
LOCUS       DAOQJE010000979         1245 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_118316_length_1245_cov_3.469748, whole genome shotgun
            sequence.
ACCESSION   DAOQJE010000979 DAOQJE010000000
VERSION     DAOQJE010000979.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1245)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1245)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1245
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_118316_length_1245_cov_3.469748"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..432
                     /locus_tag="PLT08_18495"
     CDS             <1..432
                     /locus_tag="PLT08_18495"
                     /inference="COORDINATES: protein motif:HMM:NF015909.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 2 family protein"
                     /protein_id="HNE06525.1"
                     /translation="RLPEQGQIAIFDRSWYGRVLVERLEGFCTEADWRRAYGEINQFE
                     RQLTDYGTIVFKFWMHISKQEQLRRFKERTRTNYKAWKITDEDWRNRGKWEDYIIAAE
                     EMLLKTSTPDAPWTLVEANDKYFARIKSLRTVVEKLRRALK"
     gene            739..>1245
                     /locus_tag="PLT08_18500"
     CDS             739..>1245
                     /locus_tag="PLT08_18500"
                     /inference="COORDINATES: protein motif:HMM:NF018455.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase domain-containing protein"
                     /protein_id="HNE06526.1"
                     /translation="MLTQTLKTSTKIRGLDEVLNGGVPTGGLTLLNGGPGTGKTLVGL
                     EFLVRGAQQGIPGILLTFEEHEDALHHYATTFGWDTAALETQGLLSIIGARFDPEGLL
                     SGDFDLGGILAILRHKTEKLAAKQIVIDAPDVFLRLLDNRGKERAELYKLINWLRDQK
                     LTTLMTVKA"
BASE COUNT          351 a          303 c          298 g          293 t
ORIGIN      
        1 cgcctgcccg agcagggtca gatcgccatc tttgaccgtt cctggtatgg gcgcgtcctg
       61 gtcgagcgcc tcgaaggctt ctgcaccgaa gcggattggc gccgcgccta tggtgagatc
      121 aatcagttcg aacgtcaact cactgactat ggcaccatcg tctttaagtt ttggatgcac
      181 atcagcaaac aggaacaact acgccgtttc aaggaacgca cccgcaccaa ctacaaagcc
      241 tggaagatca ccgacgaaga ttggcgcaac cgcggcaaat gggaagatta catcatcgcc
      301 gccgaagaaa tgctcttgaa aaccagcacc cccgatgccc cctggacatt ggtggaagcc
      361 aacgacaaat actttgcccg catcaagtcc ctgcgcacgg tggtagagaa attaagaaga
      421 gcgttgaagt aaaagtccat cacctgaata tcagcgttgt tttacgtctc taacaaatag
      481 taggaaaaca ctttcaaatc gtaataaaaa gcatgtgtag aaatgtaaga aagtgtataa
      541 aatatgtcta actatttatt ggttattaag ctagggtgaa ttaacagaaa gctttatgaa
      601 agttcgtctt gttcacgaac aatactttat ttttcaagtc gatagcttca tatttaagta
      661 attgggatag tctctctcca atttataacc ttggtaaaac agtatcagcc tcacctccct
      721 aaaagaaagg cggaatatat gctgacacag acgctcaaaa ccagcaccaa aataagagga
      781 cttgatgaag tcctcaatgg aggtgtgcct accggcgggc tgaccttgct aaatggcgga
      841 cccggcacag gcaaaacctt ggtgggattg gagttcttgg tgcgaggtgc gcaacaaggt
      901 atccctggca ttctgcttac ctttgaagag catgaagatg cattgcatca ctatgccacc
      961 acctttggct gggataccgc ggcacttgaa actcaaggtc tcctttcgat cattggcgcg
     1021 cggttcgacc cggagggctt gctttcaggt gattttgacc tgggcggcat tctggcgatc
     1081 ttgcgccata aaacagaaaa gctcgcagca aagcagattg tcattgacgc tccagatgtc
     1141 tttctgcgtc tgctggataa ccgcggcaaa gagcgtgccg agttatacaa gctgattaac
     1201 tggctgcgcg accaaaagct aactacccta atgacggtca aagcc
//
LOCUS       DAOQJE010000980         1244 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_118513_length_1244_cov_5.482759, whole genome shotgun
            sequence.
ACCESSION   DAOQJE010000980 DAOQJE010000000
VERSION     DAOQJE010000980.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1244)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1244)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1244
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_118513_length_1244_cov_5.482759"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(79..474)
                     /locus_tag="PLT08_18505"
     CDS             complement(79..474)
                     /locus_tag="PLT08_18505"
                     /inference="COORDINATES: protein motif:HMM:NF037081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE06527.1"
                     /translation="MASRIQALNHYRPQIEYGETAGWREVAEFLEASSTLSKADIIAA
                     LTGLQEAVIHFNLQGRGVKLEGLGTYLPNINYQGELDAAHRLDRRLKRGLNTGSFQGK
                     IRNKKNIGKTVEQIVSMWNAEHPDDPVLY"
     gene            complement(758..1075)
                     /locus_tag="PLT08_18510"
     CDS             complement(758..1075)
                     /locus_tag="PLT08_18510"
                     /inference="COORDINATES: protein motif:HMM:NF013691.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HNE06528.1"
                     /translation="MKTYYVYIMASKSRVLYTGVTNNLERRVYEHKNKLIEGFTNKYN
                     VDRLVHYEETNDVNVAITREKQIKGWLRSKKIALIESANPQWKDLSLEWYVSAHETNK
                     VSS"
BASE COUNT          290 a          281 c          284 g          389 t
ORIGIN      
        1 gtccgttcgc ccagtagttg tattcgaaca taaggtggat gttttgaatg ttcattggct
       61 tctccagtag gtactttttc agtatagcac ggggtcatcg gggtgctctg cattccacat
      121 tgaaacaatc tgttctactg ttttcccaat attcttcttg ttgcggattt tcccttgaaa
      181 agaacccgta ttcagtccac gctttaatcg tctatccaga cgatgcgccg catcaagctc
      241 gccctgatag ttgatattcg gtaaataagt gcccagccct tcgagcttaa ccccgcgccc
      301 ttgaagattg aagtggatga cagcctcttg taacccggtc agggcggcga taatgtctgc
      361 cttgctcagc gtagagctgg cttccaaaaa ctcggcaact tcccgccagc cagcggtctc
      421 gccatactcg atttgcggac ggtagtggtt gagtgcttga attcgagacg ccatgcaatt
      481 cctcctgtct tgttaatgaa caagacttgt cttaatccta atatcctgca aggatattga
      541 ttatgttgtt gcaggatatt ataaatatgc tggcagggta ttgtcaatta aacagtagag
      601 gttggggaaa cggacagtat attttggaaa accagacctc cgaggttttt aaaacctcgg
      661 aggtctggga tgcaatgtgt cttgtcattc tgagggcgcg tgttctccgc gaccgaagag
      721 tctctagggt tatggtagag atccttcgcc tatcggctca ggatgacacc ttatttgttt
      781 catgcgctga tacataccat tctaaactta ggtctttcca ctgcgggttt gccgactcaa
      841 ttaatgcaat tttcttcgac cgtaaccaac ctttgatttg tttttcacgc gtaatagcaa
      901 catttacatc atttgtttct tcgtaatgga cgagacggtc gacattgtac ttgttagtaa
      961 acccttcaat gagtttattt ttgtgctcgt acacacgccg ttcaagattg ttagtcacgc
     1021 ctgtgtaaag gacgcgtgat ttacttgcca taatatatac gtaatacgtt ttcatataac
     1081 cctctctttt gtcatcctga gggagcgttc tttgcgaccg aaggatctct gagattgttg
     1141 tagagatgct tcgcctatcg gctcagcacg acaaataatg atgtcatcct gagggagcgt
     1201 tctttgcgac cgaaggatct ctgagattat cgtagagatg cttc
//
LOCUS       DAOQJE010000981         1225 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_122325_length_1225_cov_6.173504, whole genome shotgun
            sequence.
ACCESSION   DAOQJE010000981 DAOQJE010000000
VERSION     DAOQJE010000981.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1225)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1225)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1225
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_122325_length_1225_cov_6.173504"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(144..806)
                     /locus_tag="PLT08_18515"
     CDS             complement(144..806)
                     /locus_tag="PLT08_18515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE06529.1"
                     /translation="MDTILQVFQAVGYLLLLVLLLVLWRKFVQRQEVRAFWFLLALAL
                     TINLLGNIAWIIHDMVIGKSLNTFSAIDLFYVSRYILIGIALWSYPLSLSRKAGIWTG
                     IVTVMVTVLIWVVYFPPAMKLRGGDGWGFLGVAMYPALDTALIVLAWFRVLQNKEKGT
                     FFLLLLFSMVSYGIANTLNLTGYVFPSLNSGVLPNIFWILTDVLLLIMAFRDNEIKKD
                     KE"
     gene            complement(879..>1225)
                     /locus_tag="PLT08_18520"
     CDS             complement(879..>1225)
                     /locus_tag="PLT08_18520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE06530.1"
                     /translation="ALAAGHDVSKLQGSILIDVAQKGDQMTQMNGASKEIYEGDMVMK
                     DEHGICCSIIYGQDNLSPISPETTHALYVVYTPVGVPVESVEAQLRAIEANIRLFIPS
                     LVVEQSRVLTNS"
BASE COUNT          382 a          298 c          222 g          323 t
ORIGIN      
        1 ccaacttcaa tcaagtctgc accgtttttg gcgagggctt cgatcacatc tactgatgtt
       61 tccaagtcgg ggtatcccaa cggaaagtaa ggcataaaaa taggtttctt ctgaaacgtc
      121 tcttcaattc ggctcgtcat ttttcattcc ttatcttttt ttatttcgtt gtctctaaat
      181 gccataatca atagaagcac atctgtcaaa atccaaaata tatttggtaa tacaccgcta
      241 ttcaacgaag ggaaaacata tcctgtaagg ttgagcgtat ttgctattcc gtacgacacc
      301 attgagaaaa gcaaaagcag gaagaacgta cctttttctt tgttttgtag aacacggaac
      361 caagctaaca cgatcaaagc agtatcgagc gccgggtaca tcgccacccc taagaatccc
      421 cacccatctc caccgcgaag cttcatagct ggcgggaaat acactaccca gatcaataca
      481 gtgaccatta ccgtcacaat acctgtccaa attccagctt tgcgagacaa agataaaggg
      541 tatgaccaca gtgcgatgcc aatcaatata taacgagata cataaaacag atcaatcgca
      601 gagaacgtgt ttaaagactt gccgatcacc atgtcatgaa tgatccacgc aatgtttcca
      661 agcaaattaa ttgtgagtgc taaagcaagc aaaaaccaaa atgcgcggac ttcttgtcgc
      721 tgtacaaact ttctccaaag aaccagtaaa agtactaaca gcagtaaata tcctactgct
      781 tgaaaaacct gcaaaatcgt atccattttt tctccgttgc gaatttacac cgtgcgcaaa
      841 gtaaattcta accacttttc gctgtacaaa agccactttc aagaattggt gagcacacgg
      901 ctttgttcta caacaagaga aggtataaac aaacggatat ttgcttcaat cgctcgtaat
      961 tgcgcttcca cagattcaac gggaacacct acaggtgtat acacgacata caacgcatga
     1021 gtagtctctg gtgaaatagg cgaaagatta tcctgcccat agatgatgct gcaacaaatt
     1081 ccatgctcat ccttcatcac catgtcgcct tcatatatct ctttcgaagc gccgttcatc
     1141 tgggtcatct gatcaccctt ttgagctacg tcgatcaaga tcgagccttg gagtttggat
     1201 acatcatgtc ctgccgccaa tgcaa
//
LOCUS       DAOQJE010000982         1222 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_122960_length_1222_cov_8.597258, whole genome shotgun
            sequence.
ACCESSION   DAOQJE010000982 DAOQJE010000000
VERSION     DAOQJE010000982.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1222)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1222)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1222
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_122960_length_1222_cov_8.597258"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..896)
                     /locus_tag="PLT08_18525"
     CDS             complement(<1..896)
                     /locus_tag="PLT08_18525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE06531.1"
                     /translation="MDKRTYNSQLLFEASASLAVFVLLLFYSYGIFVRAPYAGFYFNP
                     TNEKIEFIFSTNNSLLKVGDKIYQVGDFSLRQFKADRRLTLFDGVQKGDVVDIKVIRD
                     GNQVTIPWEFPGFTTQEFTNRLFNSWLLAYAFGIAGFAAQVTLRPRNLRRGLFITANY
                     LTAIFLISGMLSSWHLWESSTLLHVVAWFMMPIFIHLHWVFPQPFKRGISKFFVGFLY
                     GIACLLAIGEVFHALPRSLYALGFIIAIAGSIFLLIAHMVSQKKERPAARLLLISTII
                     SFSFSISVAIALSFGVVLDLGW"
     gene            complement(991..>1222)
                     /locus_tag="PLT08_18530"
     CDS             complement(991..>1222)
                     /locus_tag="PLT08_18530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE06532.1"
                     /translation="RYKDAHYPLCISKFIIWGVHQIGGKESDRSHREGKANGADHARL
                     SLKRELEQSDSSNIRFNLAAPNAERFICTLLP"
BASE COUNT          390 a          246 c          272 g          314 t
ORIGIN      
        1 atccagccta ggtctaaaac aacaccaaaa gacagggcga tcgcaacgct aatggagaat
       61 gaaaaactaa taatggtgga tattaagagt aagcgcgcgg caggtcgttc tttcttttgc
      121 gacaccatat gagcgatcaa aagaaaaatg cttcctgcta tcgcaataat gaatccaagt
      181 gcgtacaggc tccttggcaa agcatggaag acctctccta tcgctaaaag gcaggcaatg
      241 ccatataaga atcctacaaa gaattttgag atgcctcttt tgaagggttg cggaaacacc
      301 caatgtaagt gaataaagat tggcatcatg aaccaggcaa caacatgaag caaggttgaa
      361 ctctcccaga gatgccatga agagagcatg ccagagatca aaaagattgc cgtaaggtaa
      421 ttggcagtaa taaaaagacc tctacgcaaa ttacgtggac ggagagtaac ttgagccgca
      481 aaccctgcta ttccaaatgc atacgctaag agccatgaat tgaacagcct gttcgtaaat
      541 tcttgagtgg tgaatccggg gaattcccat ggaatagtaa cttggttgcc atccctaatg
      601 actttgatat caaccacatc gcctttttga actccgtcaa aaagggtcaa tcttcgatct
      661 gctttaaatt gtcgcagaga gaaatcacca acttggtaga tcttatcccc aacttttaga
      721 agagagttat ttgttgagaa aataaactcg attttttcat ttgttgggtt gaaataaaac
      781 cctgcgtatg gggctcgaac aaaaattcca tatgagtaaa aaagcaatag aacaaacact
      841 gcaagggatg cagatgcttc gaatagtagc tggctgttgt aagtgcgttt atccatgcag
      901 ggtccctatg cgagacatga ccagaacaaa ttttgaaagg aagggcttat catagataag
      961 tatatctagt tttaggtgca ctgtatattg ttaaggcaac aaagtacaga taaacctctc
     1021 agcatttgga gcagcgaggt tgaaacggat attggaactg tcggattgtt caagttcacg
     1081 cttcagactt aatctggcgt gatcagcccc atttgctttg ccttcgcgat ggcttctgtc
     1141 cgattccttg cccccaattt gatgtacgcc ccagataata aatttcgaaa tgcagagtgg
     1201 gtaatgtgca tctttgtagc ga
//
LOCUS       DAOQJE010000983         1219 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_123630_length_1219_cov_3.182990, whole genome shotgun
            sequence.
ACCESSION   DAOQJE010000983 DAOQJE010000000
VERSION     DAOQJE010000983.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1219)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1219)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1219
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_123630_length_1219_cov_3.182990"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(90..926)
                     /locus_tag="PLT08_18535"
     CDS             complement(90..926)
                     /locus_tag="PLT08_18535"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: protein motif:HMM:NF024626.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HNE06533.1"
                     /translation="MDTDKLIQTMVDEIKMVSGVKAIVLGGSRARGTHTPSSDIDLGI
                     YYDPSQPMDLSALGNIATRLDDEHRTDVITGFGGWGPWINGGGWLKVQSLPVDFLYKD
                     LGRIKSYIDACLNGKVEIFYQGGHPHGFVTSIYLGEVALCKPLYDPEGIIAELKGKVT
                     PYPEALQKAILASFAWEIDFSVGIAKKSIERADVNYAAGCCFRAVMCMLQVLFALNKE
                     YWMNEKGAINITDRFAIKPADFRKRVAEVYALLEASPEALEKAIYLLKGLSEDVYGLL
                     PK"
     gene            complement(952..>1219)
                     /locus_tag="PLT08_18540"
     CDS             complement(952..>1219)
                     /locus_tag="PLT08_18540"
                     /inference="COORDINATES: protein motif:HMM:NF022479.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF2721 domain-containing protein"
                     /protein_id="HNE06534.1"
                     /translation="KNLLAQIDNLRYRVYLIRNMQIFGVLSLLMCVVSMFALFFNWTD
                     GGVWSFGIALVLMIISLGISLRELQISVGALDLLLTNLEEHESK"
BASE COUNT          360 a          322 c          226 g          311 t
ORIGIN      
        1 gggcagtaat ggagattggg aagttgttgt aattaaaaca aaagacctcg gaggttttta
       61 aaacctccga ggtcttttta aattcaactt tacttcggca acagcccgta gacatcctca
      121 ctcaaaccct tcaacaaata gattgctttc tctagcgctt cgggagaggc ttctaataac
      181 gcataaactt ccgcaacacg tttacggaaa tctgcgggtt tgatggcaaa acggtctgtg
      241 atgttgatag cgcctttttc gttcatccag tattctttgt tcaacgcaaa caaaacctgc
      301 aacatgcaca tcactgcacg gaagcaacaa cccgccgcat aatttacgtc cgctcgttcg
      361 atgcttttct tggcaattcc cacggaaaaa tcgatctccc aagcaaaact ggcaagaatc
      421 gccttttgca acgcctccgg gtacggcgtg actttgccct tcaattctgc aatgatacct
      481 tctgggtcat ataagggttt gcaaagcgca acttccccca aataaattga ggtcacaaat
      541 ccatgcggat gcccgccttg gtaaaaaatt tccactttgc cattcaaaca agcatcaata
      601 taggacttaa ttctgcccaa gtctttatac aggaagtcta caggcaacga ttgaactttc
      661 aaccacccac caccattaat ccacggaccc caaccgccga agccggtaat cacatccgtt
      721 cggtgttcat catcaagccg tgtcgctata tttcctaaag cgctcaaatc cataggctga
      781 cttgggtcgt agtaaattcc taagtcaata tcagaagaag gggtatgcgt ccctctggcg
      841 cgcgagccgc ccaatacaat ggcttttacg cctgaaacca ttttgatttc atcaaccatg
      901 gtttgaataa gtttatctgt atccataacc acacactttc agatgcgatt tttatttcga
      961 ctcgtgctct tccaaattcg tcagcaacaa atccaaagcg cctactgaaa tttgcagctc
     1021 gcgcagggag attcccaacg agatgatcat caacaccaaa gcgatgccaa aactccacac
     1081 accaccatcg gtccaattaa agaacaaagc gaacatgctc acaacacaca tcaataggct
     1141 taagacaccg aatatctgca tatttcgaat gagatacacc cgataacgca agttatcgat
     1201 ctgcgctaga aggttctta
//
LOCUS       DAOQJE010000984         1218 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_123809_length_1218_cov_3.266552, whole genome shotgun
            sequence.
ACCESSION   DAOQJE010000984 DAOQJE010000000
VERSION     DAOQJE010000984.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1218)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1218)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1218
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_123809_length_1218_cov_3.266552"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(68..676)
                     /locus_tag="PLT08_18545"
     CDS             complement(68..676)
                     /locus_tag="PLT08_18545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE06535.1"
                     /translation="MKTDNDINETFSESIKKIEKELNLPRWGFEMIALRLYPKYMPRA
                     IFQSKKCIVDFHWNQERSYTEPFISTTYARVHAPLDDMFMIWNGQRHHCWHSDYWLLI
                     FLDGLTPHQITSQGYPSPSILRDFHKNNEESYIGELTAKMTLLKWNHYGDRFFNLLDL
                     QHPELWEKYSCFLKEFYEHRDEITKQKGLQPVNFDPQLYMVC"
     gene            complement(872..>1218)
                     /locus_tag="PLT08_18550"
     CDS             complement(872..>1218)
                     /locus_tag="PLT08_18550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE06536.1"
                     /translation="LATNKFKDVFSDDIQSASDFIAQFTKQNPNITFHFLSENYQTNP
                     SAEDKEYLKFNEENLDYMRAVNTLYQLVGYYLLAGQNSDARNTLNKYFPPDKASEYMS
                     AIEEDLQGLLAP"
BASE COUNT          362 a          218 c          239 g          399 t
ORIGIN      
        1 aaactgcttg agattgccgc agaatcccca gcgtcaggtg cacgctttgt tagtgcgcgg
       61 ttgttgatta acataccatg taaagttgtg ggtcaaaatt gacaggttga agcccttttt
      121 gttttgtaat ttcatctcga tgttcgtaaa attctttaag aaaacatgaa tacttttccc
      181 ataattctgg gtgttgtaaa tctaaaagat tgaaaaatct atcgccataa tgattccatt
      241 tcaataatgt catttttgcc gtcagttcgc caatgtaact ttcttcgttg tttttatgaa
      301 aatctcgaag aatagaaggg ctaggatagc cttgtgatgt tatttgatga ggagtcaaac
      361 catctaaaaa gattaaaagc caataatcag aatgccaaca atgatgccgt tgcccattcc
      421 atatcataaa catatcatca agcggagcat gcaccctagc gtaagtagta gatataaatg
      481 gctcagtata agatctttct tgattccagt gaaaatcgac aatgcatttc tttgattgga
      541 aaatagcccg aggcatatat tttggatata aacgcaatgc aatcatttcg aatccccaac
      601 gagggagatt taattctttc tcaatctttt tgattgactc agaaaatgtt tcgttaatat
      661 cgttatctgt tttcattaat tccattgatt aatatagaac ggttattatg cgagagcgca
      721 ctaacggcat gcgttacctg cgctggggcg gggacggcga agccgtccag ccaaaaaaaa
      781 atgaaaaggc gtaggaaact gcttgggatg tgcgcagact ccccagcgtc aggtgcacgc
      841 tttgttaggc gcgttttgat tttacagaac ttcatggtgc aagaagtcct tgcaaatctt
      901 cttcaatcgc tgacatatat tcacttgctt tgtctggtgg aaaatacttg tttaaagtat
      961 ttcgtgcgtc actattttgt cctgctaaca aataataacc tacaagttga taaagtgtgt
     1021 ttacggctct catataatcg agattttctt cgttgaattt caaatattct ttatcttccg
     1081 cgctcggatt agtttgatag ttttcgctca aaaaatgaaa agttatgttt ggattctgtt
     1141 ttgtaaattg agcaataaaa tctgatgccg attgaatatc atcagaaaat acatccttaa
     1201 atttattggt tgcaagga
//
LOCUS       DAOQJE010000985         1217 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_124010_length_1217_cov_3.780551, whole genome shotgun
            sequence.
ACCESSION   DAOQJE010000985 DAOQJE010000000
VERSION     DAOQJE010000985.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1217)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1217)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1217
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_124010_length_1217_cov_3.780551"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            93..698
                     /locus_tag="PLT08_18555"
     CDS             93..698
                     /locus_tag="PLT08_18555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE06537.1"
                     /translation="MNKKLPLKVAGIIFLAGLILPALLYYMYFEWEMKRFRDSLQPFD
                     ETEFLKTHYQGVDCSSLVGRVIFGDYGISAQEACIGERAWYEGNIELCNHINGCMAWI
                     AVKTGDLEICKQLKPERAPSMEDKLISDKRQNVSQYGCEQGVAIYRDTPKICDSIPYS
                     NESYKQECKEKATDGKTYLQFENSPMRPYCLEYNNDGVCVW"
     gene            883..>1217
                     /locus_tag="PLT08_18560"
     CDS             883..>1217
                     /locus_tag="PLT08_18560"
                     /inference="COORDINATES: protein motif:HMM:NF022019.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HNE06538.1"
                     /translation="MIYFIQSANDNRIKIGMSDNPVTRLRQLQTGASSQKPYTILAVV
                     PGGRRRESELHKQFKEYHSHREWFNSAPEILKFIEQETRQEIKKCRICGAFYLSRPDE
                     SDIRHHSKV"
BASE COUNT          388 a          248 c          266 g          315 t
ORIGIN      
        1 tcctgctctc aaagttttat ctcgtcccgc ccacccgcaa gtaacgcaaa ccgttgggca
       61 gttccttgca aagcaaaggt aaagaaaaaa aaatgaataa gaagttgcct ctcaaggtag
      121 cgggaattat tttcttggct gggcttattt tgccagcatt gctttactat atgtattttg
      181 aatgggaaat gaaacgattt agggactcac tccaaccatt tgacgaaaca gaatttctca
      241 aaacccacta tcaaggagtt gattgttctt cacttgtagg cagagtaatc tttggagatt
      301 atggaatatc agcccaagaa gcatgtatag gcgagcgagc atggtatgaa ggcaacattg
      361 aactttgtaa tcatattaat ggttgtatgg catggatagc agtcaaaact ggggacttgg
      421 aaatctgcaa acagctaaaa ccagaacgtg ctccctctat ggaagacaag ttaattagcg
      481 ataaacgaca aaatgtttcg caatatggat gcgaacaagg cgtcgctata tatcgagaca
      541 cgcctaaaat atgcgattca attccttatt ctaatgaaag ttacaaacaa gaatgtaagg
      601 aaaaagcgac ggatggtaaa acctatttac agtttgagaa ctctccaatg cgaccatatt
      661 gcttagagta caacaatgat ggggtatgcg tgtggtaggg cataaactta tctgtaaaca
      721 aaactgccca acacagcgtg cacccgacgt gtgggattct gcggcatttt caggcttttt
      781 cctggcttcg ggtttatact gccctcagag cgagtccaca ctccgcccac acgcgggtaa
      841 cgcaaaccgt tgggcaggct agagtatcac ttagggtaaa aaatgattta tttcattcaa
      901 tccgcaaatg ataaccgaat aaaaattggg atgtctgata atcctgtaac tcgtttgcgt
      961 cagttacaaa cgggggcttc ttctcaaaaa ccttacacca tactggcggt agtgcctggc
     1021 ggaaggagac gagaaagcga gttacataaa caattcaagg aatatcattc gcatcgcgaa
     1081 tggtttaatt ccgcacctga aattttgaaa ttcatagaac aggaaactcg tcaagaaatt
     1141 aagaaatgtc gaatttgcgg agcgttttac ctctcacgcc cagacgaaag tgatattcgc
     1201 catcacagta aagttca
//
LOCUS       DAOQJE010000986         1212 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_125077_length_1212_cov_6.821089, whole genome shotgun
            sequence.
ACCESSION   DAOQJE010000986 DAOQJE010000000
VERSION     DAOQJE010000986.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1212)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1212)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1212
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_125077_length_1212_cov_6.821089"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(153..344)
                     /locus_tag="PLT08_18565"
     CDS             complement(153..344)
                     /locus_tag="PLT08_18565"
                     /inference="COORDINATES: protein motif:HMM:NF015957.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0080135 - regulation of cellular response
                     to stress [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HNE06539.1"
                     /translation="MSDIKVLTRSKSNRMIAGVCAGLGDYLNIDPTVVRLLFVLGFFT
                     FHGAMLFVYLIMALVTPEQ"
     gene            complement(404..1159)
                     /locus_tag="PLT08_18570"
     CDS             complement(404..1159)
                     /locus_tag="PLT08_18570"
                     /inference="COORDINATES: protein motif:HMM:NF013078.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc dependent phospholipase C family protein"
                     /protein_id="HNE06540.1"
                     /translation="MGTWICHLRIAENLLPHLPEIDPITFTFGNLAPDSGIPNEDWTE
                     FDPPKEVTHFLNKGEGEGYIRDLVYYREYLANLEADKNLQKYSFRLGYFTHLICDVLW
                     YYYLDDTMQAGYRELIEKDASKAWGLFKKDWYSLDQQYNQIHKEGLFWKVIFLNPNPP
                     SFLPFVRENALHQQYDYIRKFYSEYDPYWFEERTFPYLNAATMDRLVENMTQATLFIL
                     ENIKSAKHQDLQSSFQLLPQAMQRPYEMPLGDV"
BASE COUNT          344 a          226 c          295 g          347 t
ORIGIN      
        1 tcaaacgtca aagaagaggc gttgcaaggt ttattaagta agaagaagat tcgggttgaa
       61 cgaatcgaaa aaggagagcc aactctggag gatgtctttt tagcgttggc tcgttaaaga
      121 tgtaggtcac gcttcaggcg tgacctactg tattattgtt cgggggttac cagcgccatg
      181 atgaggtaca caaataacat ggcaccatgg aaggtgaaga aaccaagtac aaataacagg
      241 cggacgacgg tagggtcgat attgagatag tcgcctagac cagcacatac acctgcaatc
      301 atgcggttgg atttgctccg cgtgagaact ttgatatcgc tcatttttgc tcctttcgtt
      361 tgagtacact tgtattacgc aaagaagcaa aaggagttgc agattaaaca tcgcctaagg
      421 gcatttcata tggacgctgc atggcttgtg gtaataactg aaatgaactt tgtagatctt
      481 gatgttttgc gcttttgatg ttctctaaaa tgaataatgt agcttgtgtc atattttcta
      541 caaggcgatc catggtagca gcgttgagat atgggaaggt tctttcttca aaccaatacg
      601 ggtcatattc actgtaaaac ttgcggatat agtcatattg ttggtgcaag gcattttctc
      661 gtacaaaagg caaaaaagaa ggtgggttag ggttgaggaa aataaccttc cagaacagac
      721 cttctttgtg tatctggttg tactgctggt cgaggctgta ccaatctttt ttgaacaagc
      781 cccatgcttt tgaggcatct ttttctatca gttcacgata ccccgcttgc atggtgtcat
      841 cgagatagta ataccagagt acatcgcaaa taaggtgagt aaaatagccc agccgaaaac
      901 tatatttttg caggtttttg tccgcttcaa gattggctag gtattcacga taatacacaa
      961 gatcgcggat atagccctca ccttcacctt tgttgagaaa atgagttact tctttcggcg
     1021 ggtcgaattc agtccaatcc tcgttgggaa tgcctgagtc tggggcaaga ttgccaaaag
     1081 tgaaagtgat tgggtcaatt tctggtagat ggggtaagag gttttcagca atgcgaaggt
     1141 ggcaaatcca ggttcccatt acttatactt tttcccttcc aaataattgg caacggaagc
     1201 atagagcagg aa
//
LOCUS       DAOQJE010000987         1206 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_126446_length_1206_cov_2.695917, whole genome shotgun
            sequence.
ACCESSION   DAOQJE010000987 DAOQJE010000000
VERSION     DAOQJE010000987.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1206)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1206)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1206
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_126446_length_1206_cov_2.695917"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(116..1057)
                     /locus_tag="PLT08_18575"
     CDS             complement(116..1057)
                     /locus_tag="PLT08_18575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE06541.1"
                     /translation="MKTFKKRNSVLVIILAVVLQVLLCCNLPSILFWSLCLNQDIFRP
                     LNTKILISACNKDRVNVTVTPDGKALFVTKASPDSWYRLEDVYLVDLSTNEKRDVSVE
                     NLDLMRRGKLLSSDLFWRWASPDYIIDLRDGHSYDLINLNQLPRLEGNKFDPKYYAYF
                     QSAEQVFILQQHSKVIALSPDFHQNPDKNVIYFLPSGSGAFGDGKNLEKLMNDLGVKY
                     KIVDFAYPYLYDTDLLSPTGKYIVRDDGIYLSETNTVAVRHSMKYSFRGWYYDESGVV
                     INKRGYYLIDLGPGLETSGFQYFYLPGPTIKLRLPMP"
BASE COUNT          375 a          259 c          235 g          337 t
ORIGIN      
        1 aaatggcgga ggcctgcggg tgcgatgaat cgagtgtgtg aatatttgcg ttgtagagga
       61 gtttcatggg atgtaccttt gatttttgat tctatcatag ggtattgcag ggaaatcatg
      121 gcataggcaa acgcagtttg atagtggggc cgggaaggta aaagtattga aatccagaag
      181 tctcaagccc tggaccaaga tcaatcaggt aatatcctct cttattgatt accactccac
      241 tctcatcgta ataccaacct ctaaaagaat atttcatgct atgacgtaca gctacagtat
      301 ttgtctcaga aagataaata ccatcatccc tgacaatata cttacctgta ggcgaaagaa
      361 ggtctgtatc ataaaggtag ggatatgcaa aatctacaat tttatatttc actccaagat
      421 cgttcattaa tttctcaagg tttttgccat ccccaaaagc gccgctcccc gaaggcagga
      481 aataaatcac attcttatca gggttttggt gaaaatcggg agataaagca atcaccttgc
      541 tgtgttgctg aaggataaaa acctgttccg cagattgaaa gtacgcataa tactttggat
      601 caaatttatt accttcaagt cgtggcaatt ggttcaaatt tatcaaatca taactatgac
      661 catcgcgcaa atctataatg taatctgggc ttgcccatcg ccaaaacagg tcggaactta
      721 aaagtttacc ccgccgcatt agatctagat tttcaacaga gacatctctc ttttcatttg
      781 tacttaaatc caccagatat acatcctcca accgatacca actatcggga cttgccttgg
      841 tcacgaacaa cgctttaccg tcaggagtaa cagttacatt cacacgatct ttattacatg
      901 cagagatgag aattttcgtg ttaagcgggc gaaaaatatc ctggttcagg cataatgacc
      961 agaacagtat ggatggcagg ttacagcata acaagacctg caaaacaaca gccagtatta
     1021 ttacaagcac tgaatttctc ttcttgaaag tcttcattat tctgaaacct cccattaagt
     1081 ctatttgtaa ttcaataaac aaatcacata aaaaaaggga cacagcggcc cttcgactcg
     1141 ctcagggcaa ggctgtgtcc ctacgggtta gatcaatttt tcgagaagcg catccagttc
     1201 ttcgcc
//
LOCUS       DAOQJE010000988         1202 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_127252_length_1202_cov_4.199651, whole genome shotgun
            sequence.
ACCESSION   DAOQJE010000988 DAOQJE010000000
VERSION     DAOQJE010000988.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1202)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1202)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1202
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_127252_length_1202_cov_4.199651"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            126..1136
                     /locus_tag="PLT08_18580"
     CDS             126..1136
                     /locus_tag="PLT08_18580"
                     /inference="COORDINATES: protein motif:HMM:NF024668.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4062 domain-containing protein"
                     /protein_id="HNE06542.1"
                     /translation="MKEQRKLQVFLSSTFTDMRDERQAAVEAILEAGHIPAGMELFAA
                     NNETQWITIKKWIDDSSAFLLVLGGRYGTIDNKTGKSYIEMEFQYARSTKKEVLALVM
                     SDAYLKAKETKFLRGASGVEQISQLAEVQNPEKLHEFRKLVRTGRIAPECNSLEELKR
                     QIILSLTQISAKENLAAWILDTPELRNARAISTMKSNLNAHKYGIKILSPAAGTKFKG
                     GLGNLTIEVTGTYEIKPPPNTIFRLFIKTNEPLSYWRHPTVVKFDENEHKWYGKIDLW
                     KGMGTHPYDAYIVAAVLDEIDEAMVGYAAKVQRETNKNIPFDTLPCHVEKSEDEVLVY
                     RE"
BASE COUNT          395 a          241 c          271 g          295 t
ORIGIN      
        1 cctacacctt atcatttttc tggcttggac ggcttcgccg tccaagcccc agcgcaggta
       61 acgcaaaccg ttagcccgct cccttgaata attgtaaata gttcacgagt gttagagagg
      121 caagcatgaa ggaacagcgt aaactacaag tatttctatc gtctacattt acagacatgc
      181 gtgacgagcg tcaggcggca gtagaagcca tcttagaggc gggacatatc cccgcaggca
      241 tggaattatt tgccgcgaac aacgagacac aatggattac tatcaaaaaa tggattgatg
      301 attctagcgc attccttctt gtgctaggtg gcagatatgg aacaattgat aataagactg
      361 gcaagagtta tattgagatg gaattccaat atgcaaggag tactaagaaa gaagtccttg
      421 ctcttgtcat gtcggacgcc tatttgaaag ccaaagaaac aaaattcctt cggggcgcaa
      481 gcggcgttga gcaaatcagt cagttagcag aagtacaaaa tccagagaag ttacatgaat
      541 ttagaaaatt ggtgcgaaca ggcaggattg cgccagagtg taactctctt gaagaactta
      601 agcggcaaat aatcctatct ttaactcaaa taagcgcgaa ggaaaatctt gcggcatgga
      661 tacttgatac cccagagtta agaaacgcaa gagcaatctc taccatgaaa tcaaatttga
      721 atgcacacaa gtacggaatt aagattttat ccccagcggc aggcacaaaa ttcaaaggtg
      781 gattgggaaa tcttacaatt gaagttacag ggacttatga aatcaaacca ccacccaata
      841 caatatttcg tctcttcatc aaaactaacg agccattatc ttattggcga catcccacag
      901 tagtaaaatt tgatgaaaac gagcataagt ggtacggaaa gattgacctt tggaaaggta
      961 tgggcactca cccatatgac gcttacatag ttgccgctgt tttagatgaa attgacgaag
     1021 caatggtagg gtatgcggct aaagttcagc gagaaacaaa taaaaatatc cctttcgata
     1081 ctttgccatg tcatgtagaa aaaagcgaag acgaagtttt agtgtatcgt gaatgatgaa
     1141 acgcgggcta acaaagcgtg cacctgacgt gtgggattct gcggcatttt caagcatttt
     1201 tc
//
LOCUS       DAOQJE010000989         1200 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_127703_length_1200_cov_6.540611, whole genome shotgun
            sequence.
ACCESSION   DAOQJE010000989 DAOQJE010000000
VERSION     DAOQJE010000989.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1200)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1200)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1200
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_127703_length_1200_cov_6.540611"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..661)
                     /locus_tag="PLT08_18585"
     CDS             complement(<1..661)
                     /locus_tag="PLT08_18585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE06543.1"
                     /translation="MKIIKNEKLIERNGKIGNWISLGALAVLAGGMYISFTHPEWFVY
                     SLVCLVAGFMLTQIGMYMGNRWGRSPRRDEKIDAAMKGMHSEFSIYHYSTPASHLLVG
                     PAGVWVILPYQQAGIAYFEKNRWKIKGGGFMQGYMRIFGQEGIGRPDIEAETEISSVR
                     KFFEKKLEGTTIPEIKSVMLFTNEQVEVEPGESPIPAMKLKDIKGFMREESKNRKLTG
                     AQ"
     gene            complement(664..>1200)
                     /locus_tag="PLT08_18590"
     CDS             complement(664..>1200)
                     /locus_tag="PLT08_18590"
                     /inference="COORDINATES: protein motif:HMM:NF014554.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase"
                     /protein_id="HNE06544.1"
                     /translation="GILGSIYAEKVRGVKNPKVGLVSNGEEEGKGNELVKGATPLFKA
                     SNLNYFGNVEGKEVIGGAVDVAVTDGFTGNVMLKTSEAVAKLITDTIRSLIKNGNLQT
                     KIGGLLVKPALGAIKKLLDPGEQGAAPMLGVNGLVFIGHGRSDAFAIKNAVRVAKEAV
                     DVKVLDAMKNAIEESLKK"
BASE COUNT          305 a          307 c          223 g          365 t
ORIGIN      
        1 tttgcgcccc ggtcaacttt cggttcttag actcttcccg cataaagccc tttatatctt
       61 tcaatttcat cgctgggata ggagattcgc ccggttcaac ctcaacctgt tcattagtga
      121 acaacatgac agattttatt tcaggaatgg tagtgccttc taattttttc tcgaaaaact
      181 tgcgtacaga tgaaatttct gtttcagcct caatatcagg gcgaccaata ccttcctgcc
      241 caaagatacg catatagcct tgcataaacc cgccgccttt gatcttccag cggttctttt
      301 cgaagtaagc aattccagcc tgttgatatg gcaatatgac ccatacgcct gctggtccaa
      361 cgagtaagtg agaggctggg gtggagtagt ggtagatact aaactcgcta tgcatccctt
      421 tcatggcagc gtcgatcttt tcgtcacggc gaggagaccg cccccagcgg tttcccatat
      481 acataccaat ctgtgtcagc ataaagccgg ctacgagaca aacaagtgaa tagacaaacc
      541 attcagggtg ggtaaatgaa atatacattc caccagccaa caccgctaag gctcctaaac
      601 taatccaatt gccaatcttt ccgtttcgtt caattaactt ttcgttttta atgattttca
      661 tgtttatttc ttgagactct cttcaattgc gtttttcatt gcatctaaca cttttacatc
      721 cacagcttct tttgccacgc ggacagcatt tttgatcgcg aaggcatcag aacgcccgtg
      781 accgataaag accaatccgt ttacgcccaa cataggagca gcaccttgtt cgccggggtc
      841 aagtaatttc ttaattgcgc ctaatgccgg tttgacgagc aaaccgccga tttttgtttg
      901 aagatttcca tttttgatga ggctgcgtat tgtatctgtg atgagtttcg caacagcttc
      961 cgaagtcttt agcatcacat ttccggtaaa accatcagta acagccacat ccactgcgcc
     1021 gccaattact tctttacctt ctacattacc aaaataattc aagtttgatg ctttgaataa
     1081 aggagttgct cccttaacca attcattacc tttgccttct tcttcaccat ttgagaccaa
     1141 gccaactttg gggttcttca cgccgcgaac tttttctgcg tagatgcttc ctaaaattcc
//
LOCUS       DAOQJE010000990         1182 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_131862_length_1182_cov_3.024845, whole genome shotgun
            sequence.
ACCESSION   DAOQJE010000990 DAOQJE010000000
VERSION     DAOQJE010000990.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1182)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1182)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1182
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_131862_length_1182_cov_3.024845"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(38..>1182)
                     /locus_tag="PLT08_18595"
     CDS             complement(38..>1182)
                     /locus_tag="PLT08_18595"
                     /inference="COORDINATES: protein motif:HMM:NF013324.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HNE06545.1"
                     /translation="QHFTFHDLVKTMDRSKESVWDAIQNLQSKKYAKNANSKFMIDFA
                     GVEYLTKRGILANEAEETPKPKDFKKKLKPPFFSNWTEMLIWLALPFSLSLIATILTI
                     YFTYGYNYVSPEKRMYQIATWIVVAVLLFFYVKLSYHHPMGRERIVVFQSGEAIGKKG
                     PGRILLLPLIQHPKKVDLSERPLEIKKEPCLTRDKMLVTAGFYMTWEIDDPILSLTKV
                     SNVEDSISSLTAAVLRSTIAEYEMNDAIETQRALNALIRTRIEHKAAEWGVQVNNTEL
                     RELQPPDGVIKSIENRRKSENNIESLRQLIAVGSRMDARTFNLKYLETLEKIGEGKST
                     KYIIPMEFFNLLQEFIRGQNNNSANTAGNNGNGNNPPEQLPGGPIQ"
BASE COUNT          303 a          238 c          270 g          371 t
ORIGIN      
        1 acttagaagc acatccagat aaattattgt ttatagatta ttgaataggt ccacctggca
       61 attgttcagg tggattattt ccgtttccgt tgtttcctgc agtgtttgcg ctattgttgt
      121 tttgccctcg aatgaattct tgtaataagt taaagaattc catagggata atatacttcg
      181 ttgactttcc ctcgcctatt ttttcaaggg tttcaagata cttaaggtta aaagtgcgag
      241 catccatgcg ggaaccgact gctatcaact gtcttaaaga ttcaatatta ttctcagatt
      301 tacggcggtt ctcaatagac ttaataacgc catctggcgg ctggagttca cgaagttcag
      361 tattgttaac ctgaacgccc cattcagcgg ctttatgctc aatgcgggta cggataaggg
      421 cattaagagc acgttgtgtt tcaatggcat cattcatttc atattctgca atggttgatc
      481 ttaaaaccgc agcagttagc gatgaaatcg aatcttcaac gttggacacc ttagttaagc
      541 taaggatggg atcgtcaatt tcccatgtca tgtaaaaccc ggctgtgacc aacatcttgt
      601 ctcgagtcag gcagggttct tttttaattt caagcgggcg ctcactgaga tctacctttt
      661 tggggtgttg aatgagcggg agtaataaaa tgcgccctgg tccctttttg ccgatggctt
      721 caccagactg gaacaccaca atccgttctc tgcccatggg gtgatggtat gacaatttca
      781 catagaagaa aagcagaacg gctacgacga tccatgtagc gatttgatac atgcgttttt
      841 cgggcgagac gtagttgtac ccatatgtga aataaatcgt tagaatggtt gcgattagcg
      901 ataagctgaa cggcagagcc agccaaatga gcatttctgt ccagttggag aagaaaggcg
      961 gcttcaactt ctttttaaag tctttgggtt tgggcgtctc ttctgcttca ttggcaagga
     1021 taccgcgctt tgtgagatat tcgaccccgg caaagtcaat cataaatttt gagtttgcgt
     1081 ttttggcgta cttcttggat tgtaaattct gtatcgcatc ccatacactt tctttggagc
     1141 gatccatggt tttgaccaga tcgtgaaaag taaagtgttg ac
//
LOCUS       DAOQJE010000991         1177 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_133047_length_1177_cov_2.434046, whole genome shotgun
            sequence.
ACCESSION   DAOQJE010000991 DAOQJE010000000
VERSION     DAOQJE010000991.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1177)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1177)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1177
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_133047_length_1177_cov_2.434046"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..189
                     /locus_tag="PLT08_18600"
     CDS             <1..189
                     /locus_tag="PLT08_18600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE06546.1"
                     /translation="QCGRALVGSFCPEHGVEGVMPPQNSEAQQPNGTTSQMVCPKCGR
                     LLSVRFCPEHGVEGVPQK"
     gene            206..841
                     /locus_tag="PLT08_18605"
     CDS             206..841
                     /locus_tag="PLT08_18605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HNE06547.1"
                     /translation="MYCTFCNHPNPEGNKFCESCGKPLAIRQQAIPPSPVSQSTVTTP
                     SQTSWARRLASIGSAIVIYGFFLPWLLVSCSLDVNKTSGVKVSGYEIASGNYKIAQNL
                     NQLGTLFGGNTYDTSSAETAYPLLAAIPLLGAIGLIALNGRVSGSVVAILTGLLGIGG
                     MAIFSITAVAYGSQLGQSMLLELSFREGFWATWFGFLWQVIVAIMTVRQRR"
BASE COUNT          339 a          254 c          268 g          316 t
ORIGIN      
        1 caatgtggac gggcattagt ggggagtttt tgtccagaac atggagtaga aggagtaatg
       61 ccccctcaaa attcagaagc gcagcaaccg aacggcacta cctcacaaat ggtttgtcca
      121 aaatgcgggc gactgctttc agttcgcttt tgtcctgaac acggcgtaga aggcgtacct
      181 caaaaataat tgtaaggagg ataaaatgta ctgtaccttt tgtaatcacc cgaatcctga
      241 aggaaataag ttttgcgaaa gttgcgggaa gccattagca ataagacaac aagccattcc
      301 accttcccct gtgtcacagt cgactgttac tactccatca cagacgagtt gggctagacg
      361 attagcctca ataggaagtg ccatagtcat atatggtttc ttcttgccct ggttgcttgt
      421 ctcctgtagt ctcgatgtta ataaaacatc gggcgttaaa gtttcaggtt atgaaattgc
      481 ctctggaaat tacaaaatcg ctcagaacct gaatcaactt ggaactttat ttggaggaaa
      541 cacatatgac acatcaagtg ctgaaaccgc gtatccactg cttgccgcca tacctttact
      601 aggtgcaatt ggtctcatag cgttaaatgg tcgtgtttct ggttccgttg tagccattct
      661 cactgggctt ttgggtatag gaggaatggc aatcttttca ataacagcgg ttgcctatgg
      721 gagtcaactt ggacagagca tgttacttga actaagtttt agggagggtt tttgggcaac
      781 atggtttggg tttttatggc aagtgatagt tgcgataatg acagtgagac aacgtaggtg
      841 aataagattg agtcttgcac aacaaaaaac gcccaacaaa gcgtgcactt gacgggtggg
      901 attctgcggc attttcaagc attttccacg cccgaacaga atcctgctct cggagtttta
      961 tctacacccg cccacccgca agtaacgcaa accgttagcc acctctctgc aaaataaaat
     1021 agtgagaata aaatgccagt aaatattcca ggaaaatcta tcaaaccacc aaaggcaact
     1081 tgtttaataa tcgttccctt tgacgccgct ggttcacgca tcaaagatac tattcagcga
     1141 gcgttagaag aaatggatat tcgggcatta agccttg
//
LOCUS       DAOQJE010000992         1162 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_136457_length_1162_cov_4.136405, whole genome shotgun
            sequence.
ACCESSION   DAOQJE010000992 DAOQJE010000000
VERSION     DAOQJE010000992.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1162)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1162)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1162
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_136457_length_1162_cov_4.136405"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..793)
                     /locus_tag="PLT08_18610"
     CDS             complement(<1..793)
                     /locus_tag="PLT08_18610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE06548.1"
                     /translation="MNKSKFRVMFLLIAMFVFAAACSLPGTSSSSDVVSTEEAATEVT
                     SITESAPPTDPAIQHTTIPIGLPEKQSGEAGDFNSVNVIANKSVIGGDRFTFGRFERP
                     FNANTMDVYFPELDIVYTQAYQDDTWIYGRIFFNDISANNPSAQYAVEIDTDLNGKSE
                     WLIIANKPSSTEWTVENVRVYQDTNNNVGGEMPMLTDKNPVSGDGFETLVFDQGKGNN
                     SDAAWVRISPEDSKLIEFAINRAALSNPSKYLINFWAGNNLDPAKF"
     gene            967..>1162
                     /locus_tag="PLT08_18615"
     CDS             967..>1162
                     /locus_tag="PLT08_18615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE06549.1"
                     /translation="MSFIKPPTKIPLFLRIGIWISEKVTGRLMLPPRVLSWYPKAAIG
                     SGVMEALVAHADGQMNQRMLK"
BASE COUNT          334 a          230 c          232 g          366 t
ORIGIN      
        1 caaatttggc aggatcaagg ttgttcccag cccaaaagtt gattaagtat tttgagggat
       61 tcgataatgc ggctcgattg atggcaaatt caataagctt agaatcttcc ggggagatac
      121 gaacccaggc agcatctgag ttgttaccct ttccttgatc aaagaccaag gtttcaaagc
      181 catcaccaga aacagggttt ttatcggtga gcatgggcat ttcaccgcca acattattat
      241 tggtatcttg atatacacga acattttcaa cagtccattc cgtggatgat ggtttattgg
      301 caataattag ccattctgat tttccattaa ggtctgtgtc aatttctaca gcgtattgag
      361 cagaaggatt atttgcagaa atatcgttga aaaagatacg accataaatc caagtgtcat
      421 cctgatatgc ctgggtgtaa acaatatcta gttctgggaa atagacatcc attgtatttg
      481 cattgaacgg acgttcaaac cgaccaaaag taaagcgatc tccaccgata actgacttat
      541 tggctatcac atttacagag ttgaaatctc ctgcttcacc actttgtttt tcgggaagac
      601 caataggaat agttgtatgc tgaattgccg gatctgtggg cggtgcagac tcagtgattg
      661 atgtaacttc cgttgctgct tcttcagttg acacaacatc acttgaagat gatgtgccgg
      721 gcaaactgca tgcagctgca aaaacaaaca ttgcgattag taaaaacata actctaaatt
      781 tacttttatt cataacactt ctcctaataa aattatgttg tcattgtatg gtttttattt
      841 tcaagtttga atggtacggc agtacttttt tgcgctaatt agtacttaag ttcttaatta
      901 tttatcaata atatgacttg agcactattc gtgaaactat aactattagg ggtttataat
      961 tcttttatgt ctttcattaa acctccaaca aaaatcccac tatttctgcg aataggtatt
     1021 tggatttctg aaaaagtcac tggtagactc atgctcccgc ctcgtgtgct ctcttggtat
     1081 ccgaaggctg ccatcggttc tggcgtcatg gaggcactcg ttgcccatgc agatggacag
     1141 atgaaccagc gtatgctcaa gc
//
LOCUS       DAOQJE010000993         1144 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_141016_length_1144_cov_5.440771, whole genome shotgun
            sequence.
ACCESSION   DAOQJE010000993 DAOQJE010000000
VERSION     DAOQJE010000993.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1144)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1144)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1144
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_141016_length_1144_cov_5.440771"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..576
                     /locus_tag="PLT08_18620"
     CDS             <1..576
                     /locus_tag="PLT08_18620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE06550.1"
                     /translation="NNNQIKTPEIERLTTYETLQAYPSPMSVYSFDGQVTDGRLMFSV
                     EVISSECNNAGEPIKINMFFQNLTTGPILITSGFNIINGGLGAGGNLTAIIFDSHKNR
                     IYTLADRNLSSDTFYVPLNEYSTIFQNASKKFATDYYFPKKIYRNKTDEIRSTPTSGQ
                     YLIRFVYSDYQRENNNWEGVVSSNLITVCIK"
     gene            complement(665..943)
                     /locus_tag="PLT08_18625"
     CDS             complement(665..943)
                     /locus_tag="PLT08_18625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE06551.1"
                     /translation="MEPKWSPRKYSTLIYAKAHDQTVAEDYFQAMQRVEQRLNASTVL
                     SARFVPVEKNIKDVKVQNVFQLIESLALPDLCQEERLEIVGALKMALD"
     gene            933..>1144
                     /locus_tag="PLT08_18630"
     CDS             933..>1144
                     /locus_tag="PLT08_18630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE06552.1"
                     /translation="MGSTSITTDTSGAKISEMRYKPWGEVRYTWKASQTTTPAYALTR
                     FTFTGQYSHIIPVGGMDDPSTSGVTEG"
BASE COUNT          349 a          249 c          232 g          314 t
ORIGIN      
        1 aataataatc agatcaagac gcctgaaatt gaaagactta cgacttacga aacgttgcaa
       61 gcgtatccct ccccgatgtc cgtgtatagc tttgatgggc aggtaacgga tggtaggctt
      121 atgttttctg ttgaggtgat ttcatctgag tgcaataatg ccggtgagcc tataaaaata
      181 aatatgtttt ttcaaaactt aacaacaggg cctattttaa taacttcagg gttcaatata
      241 ataaatggcg gtttgggcgc aggaggaaac ctaacagcaa ttattttcga cagccataaa
      301 aaccgaatct atactcttgc cgatagaaac ctaagtagcg acacatttta cgttcctcta
      361 aatgaatact caacaatttt ccaaaatgca tctaaaaaat tcgctacaga ttactatttt
      421 cctaaaaaaa tatatagaaa caagactgat gaaattcgtt caactccaac gtcaggacaa
      481 tacttgatta gatttgttta ttcagattac cagagagaga ataataactg ggaaggtgtt
      541 gtaagctcta atttaattac ggtgtgtatt aaataaaggg caacgtaact ttttgtaatg
      601 gtatttgtct ctctgctaag taatcagttg cgtattttag aaaacaatta ttaaagtgca
      661 acaactaatc taaagccatc ttgagcgctc ccacaatctc caagcgctct tcttggcaaa
      721 gatctggcaa tgccagactt tcaatgagtt gaaaaacatt ttgaacctta acatccttga
      781 tattcttttc tactggcaca aaccttgcgc tgagcaccgt cgaagcgttt aatctctgct
      841 ccaccctctg catggcttgg aaataatcct ccgccaccgt ctggtcgtgt gccttggcat
      901 aaatgagcgt ggaatacttc ctgggagacc atttgggttc cacttccatc accaccgata
      961 caagcggcgc gaagatctcg gagatgagat acaaaccctg gggcgaagtg cgctatacgt
     1021 ggaaggcgag tcagaccacc acaccggcgt atgccctgac ccgcttcacg tttacgggac
     1081 aatattccca tatcatcccc gtagggggta tggatgatcc cagcacaagc ggagtcaccg
     1141 aggg
//
LOCUS       DAOQJE010000994         1142 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_141558_length_1142_cov_3.613615, whole genome shotgun
            sequence.
ACCESSION   DAOQJE010000994 DAOQJE010000000
VERSION     DAOQJE010000994.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1142)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1142)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1142
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_141558_length_1142_cov_3.613615"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(236..>1142)
                     /locus_tag="PLT08_18635"
     CDS             complement(236..>1142)
                     /locus_tag="PLT08_18635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="VCBS repeat-containing protein"
                     /protein_id="HNE06553.1"
                     /translation="ISSSFLHLIIFLAGRLRQVHAPGQVTQTVKNFIISLLYIFTCVW
                     ISGCSNIPVATTNPTSHSEPQFAVSKKTSLEVYRNGKAYEILIGYSMGRAPFYATPVG
                     ETPKTWSENYADGAQIEAFSDFDNDGEMEILVSAFVCGANCSDRLMVYEYDSVNDTYF
                     VSDEIWARLREYTDFNKDGTPEFTLYEYGFCFRCSNASNALSALAILQYKEGKFVDVS
                     LEFPEQIQEHADRFLNSAINNEQDAAHIYLPGYLYDMYRLGKMEEGRVVFDEVCNNVL
                     KPYSIDCASFRMEVEKSILEYKFRE"
BASE COUNT          344 a          240 c          224 g          334 t
ORIGIN      
        1 cacgtagcat aaaattgatt aatccgatta atcgtatttt acgcccgtga aagccttcgg
       61 tcaaggattt ttggttaaga gtttagttaa ttcaagagga aataaaagcg ttgcgtggac
      121 ttggcttatc cgtccagcca gaaaaaggct aaggcgtaag aaactgcttg agactgtgca
      181 cacgcaaagc gccccagcgt caagtgcacg ctttgttagg cgtttttgat tggtatcact
      241 cacgaaattt gtattctaaa attgattttt caacttccat acgaaaactc gcacaatcaa
      301 tagaatatgg cttaaggaca ttgttgcata cttcatcaaa aacgaccctt ccttcttcca
      361 ttttacctag acgatacata tcatataagt aacctggcaa gtatatatgg gcagcatctt
      421 gttcattgtt tattgcagaa ttaagaaacc tatctgcatg ttcttgaatc tgttctggga
      481 attctaggct tacatctaca aattttcctt ccttgtactg caaaattgcg agagcggata
      541 gcgcatttga ggcattactg catcggaaac aaaacccata ctcgtaaaga gtgaactcag
      601 gtgttccatc tttattgaaa tcagtatatt cccttaatct tgcccaaatt tcatctgaaa
      661 caaagtaggt gtcattgacg gaatcatatt catacaccat caaccgatca ctgcaatttg
      721 caccacatac aaaagccgaa acaagaattt ccatttcgcc atcattgtca aaatcagaaa
      781 aggcttctat ttgtgcgcca tctgcatagt tttctgacca tgttttcggt gtttctccaa
      841 ctggcgtagc ataaaaaggc gcacgcccca tgctgtagcc aatgagaatt tcataggctt
      901 ttccattgcg ataaacttcc agagatgtct ttttagaaac cgcgaattgt ggttcagaat
      961 gactggtagg attagtggtt gcaactggaa tgttgctgca accactaatc cagacacatg
     1021 taaaaatata cagcaaggaa atgatgaaat tcttcacggt ttgcgttacc tgccccggtg
     1081 cgtggacttg gcgaagccgt ccagccagaa aaatgataag gtgtagaaaa ctgcttgaga
     1141 tt
//
LOCUS       DAOQJE010000995         1128 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_145246_length_1128_cov_2.983225, whole genome shotgun
            sequence.
ACCESSION   DAOQJE010000995 DAOQJE010000000
VERSION     DAOQJE010000995.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1128)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1128)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1128
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_145246_length_1128_cov_2.983225"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(282..602)
                     /locus_tag="PLT08_18640"
     CDS             complement(282..602)
                     /locus_tag="PLT08_18640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE06554.1"
                     /translation="MAKVKLNPALQQIRGRLAGFVYRIRYGEQTISKSPDMSKVKWSA
                     AQVEHRRRFKLAVAYAHLAMGDEKVRLQYMAEAAEKGKRPFDLAVSDYFKGRNLLMKS
                     ESSK"
     gene            complement(746..>1128)
                     /locus_tag="PLT08_18645"
     CDS             complement(746..>1128)
                     /locus_tag="PLT08_18645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE06555.1"
                     /translation="MQRLMKNLFLPNGGWARNVLMDATNWGEVLHRLPRVGNRMLERL
                     EKNEPFRMEVKDTDRILAKLDRLVTRLSLSLLVAAFVVGLPLLIPLTTPDSLPRWLLL
                     VGMVPIIGTGVWLGFSLLNTPKNK"
BASE COUNT          277 a          299 c          249 g          303 t
ORIGIN      
        1 acccgcagat tctgactccg ctcggcagtg attatcacgg ctggcaaagt cacatctacg
       61 tgcctgcgga tgtctgtcca ttcaggtaac aactcaggtg acagtcagct tgaagctgac
      121 tgtcacctct ttggcaaata ataaaacatc acctctcaag acatagggag aggtgttttc
      181 tttttcgtgt ctccctgagc gatagcgaag ggtttcttgg ataatcgtag aggttcttcg
      241 ctccgctcag aacgacatcg cctaaggtga gttatatcaa atcacttact actttcgctc
      301 ttcatcaata aatttctccc tttaaaataa tccgagaccg ccaggtcaaa tggacgcttg
      361 cccttttcag cagcttctgc catgtattgc agccttactt tctcatcgcc cattgccagg
      421 tgtgcgtagg ctactgcaag cttaaagcgg cgtcggtgct ccacctgtgc cgcgctccac
      481 ttcactttgg acatatcagg cgatttcgaa atggtctgct ctccatatcg tattcggtac
      541 acaaatcctg ccaatcgtcc gcggatttgt tgaagagccg ggtttaattt cacttttgcc
      601 atatttcctc catggtctca cccataatcc tgtactaatc ctgtactgat cccgtagtat
      661 aagtctagtt atagtccaag tatgagcccc agaatcacgc tcacctttcc aagtcattgg
      721 gggcgttttc tggttcagta gattgttatt tatttttggg ggtattcaac agagaaaagc
      781 ccagccagac tccagtacct atgatcggaa ccatgccaac caaaagcagc caccttggca
      841 ggctatcagg agtagtgagc ggaattaaca gcggcagacc caccacaaaa gcagcaacaa
      901 gcaaactcaa agaaagacgc gtaaccagtc ggtctaattt tgccagaatg cggtctgtgt
      961 ctttcacttc catgcggaag ggctcgttct tctctaggcg ttccaacatg cggtttccaa
     1021 cacgcggcaa gcggtgcaga acctcgcccc aattggtggc atccatgaga acattgcggg
     1081 cccatccgcc gtttggaagg aaaagatttt tcatcaagcg ctgcatgt
//
LOCUS       DAOQJE010000996         1123 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_146490_length_1123_cov_7.117978, whole genome shotgun
            sequence.
ACCESSION   DAOQJE010000996 DAOQJE010000000
VERSION     DAOQJE010000996.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1123)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1123)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1123
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_146490_length_1123_cov_7.117978"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..598
                     /locus_tag="PLT08_18650"
     CDS             <1..598
                     /locus_tag="PLT08_18650"
                     /inference="COORDINATES: protein motif:HMM:NF014344.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="arginine deiminase family protein"
                     /protein_id="HNE06556.1"
                     /translation="IEGGDVAMFKGYCFIGVGARTNMSAVKEVYRQIGASLQKHHIEL
                     IAVVNKQHEEDSNFFISPTSEHMQVMHLDMFWIPLTQDLVMAYTTELDHREVVRVWQE
                     KGRILTERLGTFREFMSIIGVEIFEVDEAEQKNFATNLLNFGNKKVIAALSSNPRVNA
                     ELERRGFTVKYAELTKLVDGYGAVHCLTAPLKRRIPNS"
     gene            711..>1123
                     /locus_tag="PLT08_18655"
     CDS             711..>1123
                     /locus_tag="PLT08_18655"
                     /inference="COORDINATES: protein motif:HMM:NF014669.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter BioY"
                     /protein_id="HNE06557.1"
                     /translation="MTTLAPTIYNRTFPRVSEVVRDITLILLGSWLIALFAQIEIPLP
                     FSPVPITGQTFAVLLVGALLGSKRGAAAMVAYIAQGAAGLPFFAGGASGFGILTGATA
                     GYLVGFIVAAYVVGLLAERGMERSFKTSIVPFLVGT"
BASE COUNT          277 a          284 c          270 g          292 t
ORIGIN      
        1 aattgaaggc ggcgatgttg ccatgtttaa aggctactgc tttatcggcg tcggtgcgcg
       61 caccaatatg agcgcggtca aagaagtgta ccgccaaatc ggggcgtcat tgcaaaagca
      121 ccatatcgaa ctgatcgctg tcgtcaacaa acagcatgaa gaagatagca atttcttcat
      181 cagccccact agtgaacaca tgcaagtcat gcatttggac atgttctgga ttccacttac
      241 acaggatttg gtcatggcat acacgactga actggatcat cgcgaagttg tgcgtgtatg
      301 gcaggaaaaa gggcgtatct taacggaacg attaggaact ttccgtgaat ttatgtccat
      361 aatcggtgta gaaatcttcg aagtggacga agccgaacaa aagaacttcg ccacgaattt
      421 actcaacttt ggcaacaaaa aagtgatcgc ggcattatct tccaacccgc gcgtgaatgc
      481 agagttggaa agacgtggct tcacggtcaa atacgccgag ttgaccaaac tggtcgatgg
      541 atacggcgca gtccactgtc tcaccgcacc gctaaaacgc cgtataccaa attcataaga
      601 atggcatgtc atcctacagt ggggacagaa tgacattaat ccctatccaa atcttcacaa
      661 gggtttgcgt gttataacta ttgacattct acttaggaga tagattttat atgaccacac
      721 ttgcaccaac catctacaac cgcacctttc ctcgtgtttc tgaggtcgtg cgcgatatca
      781 ccctcatctt actcggttcg tggttgatcg cgctttttgc tcaaatcgaa attccactcc
      841 ccttctctcc tgtcccaatt actggtcaga cgtttgccgt attgctggtc ggtgccttgc
      901 ttggctccaa gcgtggcgca gctgccatgg tcgcttatat cgctcagggc gcggcagggt
      961 tgcctttctt cgcgggcggc gccagcggat ttggcattct caccggcgca accgctggtt
     1021 acctcgttgg ctttattgtg gcagcgtatg ttgtaggtct gcttgcagaa cgcggcatgg
     1081 aacgaagctt caaaacctcc atcgttccat ttttggtcgg cac
//
LOCUS       DAOQJE010000997         1123 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_146667_length_1123_cov_2.048689, whole genome shotgun
            sequence.
ACCESSION   DAOQJE010000997 DAOQJE010000000
VERSION     DAOQJE010000997.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1123)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1123)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1123
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_146667_length_1123_cov_2.048689"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..874)
                     /locus_tag="PLT08_18660"
     CDS             complement(<1..874)
                     /locus_tag="PLT08_18660"
                     /inference="COORDINATES: protein motif:HMM:NF025056.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003953 - NAD+ nucleosidase activity
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="toll/interleukin-1 receptor domain-containing
                     protein"
                     /protein_id="HNE06558.1"
                     /translation="MDKRPLKVFLCHAHADRDPVRGLYTRLTQDGVDAWLDKANLLPG
                     QDWELEIRKAVREADVVVVCLSKQFNQAGFRQKEVRLALDTAMEKPEGEIFIIPARLE
                     ECDNLESLRKWHWVDLFEDDGYDMLIRALRARADRIGAILQIKKSWLPKITLPLSKLV
                     KPIEENNLELPKKKTLKKRMVSPDEPPPDNFPDGWDDVWKKGFESASNVEKNKLEKKE
                     DIVETPVEKKLEKSEPKKEIQAPKNFNKNTFGIIGFIVLVLVSVFGLPSLIGKPEKPS
                     STATNTVSVKTFTPI"
     gene            complement(906..>1123)
                     /locus_tag="PLT08_18665"
     CDS             complement(906..>1123)
                     /locus_tag="PLT08_18665"
                     /inference="COORDINATES: protein motif:HMM:NF014043.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF86 domain-containing protein"
                     /protein_id="HNE06559.1"
                     /translation="RAVEMNFIIIGEAANQIPEEVEEKYTAIPWTLMRAMRNRIVHVY
                     FKVDEKLMWDTIQTDLPPLIPELEKLL"
BASE COUNT          280 a          252 c          243 g          348 t
ORIGIN      
        1 gaatcggagt aaaagttttt acacttactg tatttgttgc cgtggatgat ggtttttctg
       61 gcttgccaat caaagaaggc aagccaaaga ctgaaaccaa aacaagcaca ataaaaccaa
      121 taatcccaaa ggtattttta ttaaaattct tcggcgcttg aatttctttc tttggttcgg
      181 atttttctag ttttttctca acaggcgttt caacaatgtc ttcttttttt tcgagcttat
      241 ttttttctac attagatgcc gattcaaaac cttttttcca aacatcatcc caaccatcag
      301 gaaaattatc tggaggcggt tcgtcaggag ataccattct tttcttcagt gtttttttct
      361 taggcagctc aaggttattt tcttcaatag gcttcacaag tttagaaagt ggtaacgtaa
      421 tttttggcaa ccaacttttc tttatctgta atatcgcccc aattctatct gcccgcgccc
      481 gcaaagctcg tatgagcata tcatagccat cgtcttcaaa caaatccacc caatgccatt
      541 ttcgtaagct ttcgaggttg tcacattctt ccagccgcgc agggatgata aatatctcgc
      601 cttcgggttt ttccattgcg gtgtcgaggg caaggcgcac ttccttttgt cgaaaccccg
      661 cctggttgaa ctgctttgaa aggcaaacca ccaccacgtc cgcttcgcgt acagccttgc
      721 ggatttccaa ttcccaatcc tgccctggga ggaggttggc tttgtcgagc cacgcgtcca
      781 caccgtcttg ggtcaggcgg gtgtaaagtc cgcgcacggg gtcacggtcc gcgtgggcgt
      841 ggcagaggaa gactttgagg gggcgtttat ccataaggat gagagatgaa ttatgaagga
      901 tgaaattata gaagtttttc gagttcgggg attaatggcg gcagatcagt ttggatggta
      961 tcccacatga gtttttcgtc aactttaaag taaacatgca caatgcgatt ccgcatggca
     1021 cgcatgagag tccacgggat tgctgtatat ttttcttcaa cttcttcggg aatttgattt
     1081 gcggcttcac caataatgat gaaattcatt tcaaccgcac gaa
//
LOCUS       DAOQJE010000998         1118 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_147851_length_1118_cov_5.344309, whole genome shotgun
            sequence.
ACCESSION   DAOQJE010000998 DAOQJE010000000
VERSION     DAOQJE010000998.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1118)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1118)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1118
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_147851_length_1118_cov_5.344309"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            210..767
                     /locus_tag="PLT08_18670"
     CDS             210..767
                     /locus_tag="PLT08_18670"
                     /inference="COORDINATES: protein motif:HMM:NF039497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5673 domain-containing protein"
                     /protein_id="HNE06560.1"
                     /translation="MYAVIGIIAIIPILGFGAPTYDSLNISYGILFGTIFCWVYFKLF
                     SNKQKAGKELLDIAPIQNGVILFIIGIITIILGLLGFVDFIGKDTRFSWFISTVIGLA
                     SGVYFVYMGFSRIQIYENGILVYVDLIKWSKIESFEWVDDKGKACTLKLRYKGRTPAF
                     LRNGAVPVPVEKKEQVDSLLEQFLS"
BASE COUNT          301 a          199 c          251 g          367 t
ORIGIN      
        1 ttttgaggtg ctcgactctc attttacttg gtcacatttt ccaaagcagg tcagctatct
       61 gaattatctt ctccgtttag atatttattt gttattccca tcgaatttgg aataattgta
      121 ttagtggcat ttgcattaaa gaaaatcggg gtgaatgtta aaactgcttg gttacaagga
      181 attaagaacg cttttttata tctttaataa tgtatgcagt tattggaata atagccatta
      241 ttccaatttt gggatttggg gcaccaacgt atgatagcct taacatttcc tacggaatcc
      301 tattcggaac aatattttgt tgggtttatt tcaaattgtt ttccaataaa cagaaagcgg
      361 ggaaagagtt attagacatt gccccgattc aaaacggggt aatacttttt attataggta
      421 ttattactat tattttaggc ttgttaggat ttgttgattt tattgggaaa gatacaagat
      481 tttcctggtt catttctaca gttattggtc tggctagcgg ggtatatttt gtgtatatgg
      541 gattcagtcg catacaaatt tatgagaatg gaattttagt gtatgtggat ttaataaagt
      601 ggagcaaaat tgaatctttt gaatgggttg acgataaggg aaaggcgtgt acgcttaaat
      661 taagatacaa aggtagaacg cctgcattcc tacgtaacgg cgcagtacct gttccagttg
      721 aaaagaaaga gcaagtggat tctttgcttg aacaattttt gtcgtaagta gttttaatgt
      781 aaaatcgcgc ctaacaaagc atgcacctga cgctggggat tgtcaagcgc caatgcgttt
      841 tgactgggcg atgctcgctt gaggctcgct cacccaatct gcggcaatct caggcagttt
      901 tctacgcctt agcatttttc cctggactgt cgtccaggac aggctggttg gacggcttcg
      961 ccgtccccgc cccagcgcag gtatacccta cagggacgat ccctctacga ggactttggc
     1021 gcatgccgtt agaccccttc gtgtaaaaac aagttttggg tggttgatat tacataagat
     1081 gtcgcatata ataccagcat gaagccgccc gatattgt
//
LOCUS       DAOQJE010000999         1116 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_148370_length_1116_cov_5.987747, whole genome shotgun
            sequence.
ACCESSION   DAOQJE010000999 DAOQJE010000000
VERSION     DAOQJE010000999.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1116)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1116)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1116
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_148370_length_1116_cov_5.987747"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..946)
                     /locus_tag="PLT08_18675"
     CDS             complement(<1..946)
                     /locus_tag="PLT08_18675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE06561.1"
                     /translation="MTLTPSDYREETLKPFFNYIKGGESFFIIGAPSVGKTRLVDFVM
                     GDDSDARRMGIDIDRDRAKKQYLGSAASKVWLVRVDMNRIRLEHSWGFQFFELMLHSL
                     LLACDRNSTISDIEFLKENLAGLDSKVIQSKDDLMAHRLFEMAVNRICQIYNIQICFL
                     FDEFDETYRTMPIETFAQLRAIRDSNKYMLSYILFLRNLPEKLRNPKDNESFYELISR
                     NVLGLGPYAKKDALNIIEQLEKRRDHVLPSEYRDWVYTTSGGHPGLIQALFTLLKENP
                     SAPSKMQDPEWFVKQEIIREEFRKIWNGLIEEEQTALQS"
BASE COUNT          287 a          224 c          268 g          337 t
ORIGIN      
        1 tactttgtaa ggcggtttgt tcttcttcta tcaacccgtt ccaaattttt ctgaattctt
       61 cacgaataat ctcttgtttt acaaaccatt cagggtcttg catcttagat ggggcggatg
      121 gattttcttt caaaagagtg aaaagagcct ggatcaagcc agggtgacca ccactagttg
      181 tgtaaaccca atctcggtat tccgatggaa gtacatgatc gcggcgtttt tccagctgct
      241 caataatatt aagtgcatct tttttggcat acggacccag tccaaggaca ttacgggaga
      301 ttagctcata aaagctttca ttatctttgg ggttgcgaag tttttctggg aggtttcgaa
      361 ggaaaagaat gtaagaaagc atgtatttat ttgagtcgcg gattgcccga agctgggcaa
      421 aggtttcaat tggcatggtg cggtaggttt cgtcaaattc atcgaagaga aagcaaattt
      481 gaatattgta gatctgacaa atacgattca ctgccatttc aaatagacga tgtgccatta
      541 ggtcatcctt gctttggata acttttgagt ctagccctgc aaggttctct tttagaaact
      601 caatatctga gatggtacta ttccggtcgc atgccaaaag cagactgtgt agcatcagct
      661 cgaagaattg gaatccccag ctatgctcaa gcctgatgcg gttcatatcc acgcgcacca
      721 gccagacttt tgaagcggct gatccaaggt attgtttctt tgcgcggtca cggtcgatat
      781 caatacccat gcggcgcgca tctgagtcat cgcccattac gaaatcgact agtcgggttt
      841 ttccgacact gggagcgccg attatgaaga aagattcgcc tccttttatg taattgaaaa
      901 agggttttag tgtttcttcg cgatagtctg aaggggtgag tgtcatgatt tctccgtttg
      961 gttaagttca ttcagtgggt gaccaggttt ggtcccagtg gtcgaggcgg tgacagatta
     1021 agccggcaag caaaagtgga tatccacttt tgcttttgtc tattttatgc aaaagctgag
     1081 cgcgaaaggt catgttataa agcagcccgt ataagt
//
LOCUS       DAOQJE010001000         1113 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_149219_length_1113_cov_4.215501, whole genome shotgun
            sequence.
ACCESSION   DAOQJE010001000 DAOQJE010000000
VERSION     DAOQJE010001000.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1113)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1113)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1113
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_149219_length_1113_cov_4.215501"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..262
                     /locus_tag="PLT08_18680"
     CDS             <1..262
                     /locus_tag="PLT08_18680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE06562.1"
                     /translation="GKASGERNAPVFRESVDSTLYLDHIGIFNKEEFVRLNEEYKAAF
                     LADARRQPIHAEQQVRMKEVETYLKQRTDTKWILVEKYEWES"
     gene            329..877
                     /locus_tag="PLT08_18685"
     CDS             329..877
                     /locus_tag="PLT08_18685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE06563.1"
                     /translation="MPYDPSDPTARDSALQPSEQRRLEEANFYFENNKPAQAAPIFAK
                     LAEVLRSAGQPKRAASLHSQAALAFARSRNESALVQARAAMSLLLQYKLEAQASAFYK
                     ELMREFTKRGMQPTAEAFAKEFGGQIQQPAEQDAHAAHQLDRLPSHCPSCGASLRERD
                     IRWTDADTIECGFCGTPIRPSV"
     gene            920..>1113
                     /locus_tag="PLT08_18690"
     CDS             920..>1113
                     /locus_tag="PLT08_18690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE06564.1"
                     /translation="MKPKMMLGLCLLVGLTLLVGCGPKETPLSGPERDAVLAFSEAKT
                     DNLLAGMNANDYAAFSQDFD"
BASE COUNT          281 a          268 c          314 g          250 t
ORIGIN      
        1 cgggaaagca agtggggagc gcaatgcccc tgtatttcgc gaaagcgtgg atagtacact
       61 ctatctggat catatcggta ttttcaacaa ggaagaattt gtccgtttga atgaggagta
      121 caaagcagcg tttctggcag atgccagacg gcagccgatt catgctgagc agcaagtgcg
      181 catgaaagag gttgagacgt atcttaaaca gcgcaccgat actaagtgga ttttggtcga
      241 aaaatacgag tgggaaagct aacaaaaagt gttgagatga gaaaaatgta ggataacccg
      301 tggcttgatt tcgtgagaga atgtctgcat gccatacgat ccctctgacc ccactgcacg
      361 cgatagcgcc ctacaacctt ctgaacaacg ccggctcgaa gaagctaatt tctattttga
      421 gaacaacaag ccagcccaag cggcgccgat ctttgccaag ctggcagagg tgttgcgttc
      481 tgccgggcag cccaagcgtg cggcaagttt acattctcaa gccgcgctcg cctttgcccg
      541 cagccgaaat gagtccgcgc tggtgcaggc tcgggcggca atgtctttgt tgctgcaata
      601 taagctcgaa gcgcaagcgt ccgcgtttta taaggagttg atgcgtgaat tcaccaagcg
      661 gggaatgcaa cctacggctg aggcgtttgc caaagagttc ggtgggcaga ttcagcaacc
      721 ggcggagcaa gatgcgcatg cggctcatca gctagaccgc ctgcctagtc attgtcccag
      781 ttgtggggcg tctctgcgtg agagggatat ccgctggacg gatgcagaca ccatcgaatg
      841 tggcttttgc ggcacgccca ttcgcccgtc tgtttaaaac gtaacatagc ggtcttataa
      901 aaaaggataa tgaattgcca tgaaaccaaa aatgatgctt ggattgtgcc tgttggtggg
      961 tctcaccttg ctggttgggt gtggaccgaa agaaacgcct ctctctggac cggaacggga
     1021 tgccgtcctc gcctttagcg aagccaagac ggacaatctg ctggcaggga tgaatgccaa
     1081 tgattatgcc gccttctcgc aagactttga cca
//
LOCUS       DAOQJE010001001         1107 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_150859_length_1107_cov_3.867871, whole genome shotgun
            sequence.
ACCESSION   DAOQJE010001001 DAOQJE010000000
VERSION     DAOQJE010001001.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1107)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1107)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1107
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_150859_length_1107_cov_3.867871"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            176..472
                     /locus_tag="PLT08_18695"
     CDS             176..472
                     /locus_tag="PLT08_18695"
                     /inference="COORDINATES: protein motif:HMM:NF013691.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HNE06565.1"
                     /translation="MAISKQYCVYIMTNAHNTALYTGVTNNLARRVYEHKNGLGSKFV
                     KKYNVHKLVYYEIGNDVNAAIAREKQIKGGSRKKKVDLVNNMNPQWKDLYEEIS"
     gene            605..>1107
                     /locus_tag="PLT08_18700"
     CDS             605..>1107
                     /locus_tag="PLT08_18700"
                     /inference="COORDINATES: protein motif:HMM:NF012900.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylanthranilate isomerase"
                     /protein_id="HNE06566.1"
                     /translation="MTVIKICGIKTLKDALAAIDSGADYLGFNFYSKSVRFIEKSACA
                     EITSVLKREHPQVKLVGVFVNSSVEEIKDILQTCHLDLAQLHGDETPETFAQLAPHAF
                     RAFRGIPESNGGYERDEAPAMLIDAAVKGVYGGSGVTADWTVAAKLAKQYPLLLAGGL
                     TPENVADA"
BASE COUNT          331 a          247 c          268 g          261 t
ORIGIN      
        1 aagtgagaga actttctgga atgtgatatg tcgttgcgag ggcgctcttg ttctttgccc
       61 gaagcaacct ccttgttaat gggagcgctt aagtaaatga agtaatctcc taaaacaggg
      121 gattgcttcg tcgggaaaag caccctcctc gcaatgacat gaagtggagt tacgtatggc
      181 aataagtaaa caatattgcg tttacatcat gaccaatgca cacaatactg ctttgtacac
      241 tggtgtgacc aataatttag cacgcagggt gtatgaacac aaaaacggct tgggaagcaa
      301 gtttgtcaaa aaatacaacg tccacaaact tgtgtattac gaaatcggaa atgatgtcaa
      361 tgcggcaatt gcaagagaaa agcaaatcaa aggcggttcg cgcaagaaga aggtcgactt
      421 ggtcaataac atgaacccac aatggaaaga cttatatgaa gaaatatcct aaagatggtg
      481 tcgttgcgag ggcgtacttg ttccctgccc gaagcaacct tcttgtaact gagaacgctt
      541 caataaatga gagattgctt cgtcggggag agcgccctcc tcgcaatgac atggtaaaaa
      601 gattatgacg gttataaaaa tctgtggaat aaaaacattg aaagacgcat tagcggcaat
      661 agactctgga gcagactatt taggttttaa tttttactct aaaagcgttc gcttcattga
      721 gaaatctgct tgtgctgaga tcacatccgt attgaagcgt gaacatccgc aggttaaact
      781 ggttggcgtg tttgtcaatt catcagttga agaaatcaaa gacatcctgc aaacctgtca
      841 ccttgacctc gctcaactcc acggagacga gacgccagag acatttgccc aacttgcccc
      901 gcacgctttc cgtgcctttc gcggaatccc tgaatcaaat gggggttatg aaagagacga
      961 agcgcctgcc atgttgatag acgccgccgt caaaggcgtc tacggtggaa gcggagtcac
     1021 cgccgattgg acagtcgccg caaaattggc aaagcaatat ccgctcctgc tcgcgggcgg
     1081 actcaccccc gaaaacgtcg cggacgc
//
LOCUS       DAOQJE010001002         1104 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_151734_length_1104_cov_2.861773, whole genome shotgun
            sequence.
ACCESSION   DAOQJE010001002 DAOQJE010000000
VERSION     DAOQJE010001002.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1104)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1104)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1104
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_151734_length_1104_cov_2.861773"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..554)
                     /locus_tag="PLT08_18705"
     CDS             complement(<1..554)
                     /locus_tag="PLT08_18705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE06567.1"
                     /translation="MKNSVFSIDNLPRLLWGLALLTLPVTSFRWFPGLGEGALVRPLA
                     LYPLAFLLPLLLIQAWRKKIQLTWAGAFVALGAFILFVFFSSAIGSLINPISLRGQVY
                     NGRTIRALLTLFIGIAFFVSAVWMNKNEEDLRFTVRWIFAGLCLDIAWSGLQAVTFYT
                     GLLEKEMVTHWQLAFSMRELVRTNR"
     gene            complement(551..>1104)
                     /locus_tag="PLT08_18710"
     CDS             complement(551..>1104)
                     /locus_tag="PLT08_18710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE06568.1"
                     /translation="KAWRFWVLGAIIGSAIGAAFYYIVPPPYRAKAIVNVDFNFEEAF
                     PANTDRQDFYYLEREARKMVELAWSDDVLNELNAPVKELREGKLTLSQPAEAGWHFYA
                     DDQNPQKAEELASAWANAFVEKTQTEIQSGNLNEFIKLEVTQSANLPKQRSVPLANYL
                     LTGSSGFLLLAMFAVLFIKPKAK"
BASE COUNT          331 a          251 c          265 g          257 t
ORIGIN      
        1 cgattcgtgc gaacgagttc gcgcatcgaa aaagcaagct gccagtgggt gaccatttct
       61 ttttcaagca agccagtata aaaggtgacc gcttgcaaac cgctccaggc gatatcaaga
      121 catagacctg caaaaatcca tctgacggtg aagcgcaggt cttcttcgtt tttattcatc
      181 catacggcac tgacaaagaa ggcaatgcca ataaacaacg ttagcaaggc tcgaatggtg
      241 cgaccgttat acacttgccc gcgcaacgaa attgggttga taagcgaacc aatcgccgaa
      301 gaaaaaaaca caaataggat aaaagcaccc agagcaacaa atgcgcctgc ccatgtaagt
      361 tgaatctttt tacgccaggc ttgaattaag agcaggggca ataaaaacgc caatggatac
      421 aatgctagtg gacggacgag cgcaccttcg cccaaaccgg ggaaccaccg gaaactggtg
      481 acaggcaggg ttagcaaagc cagtccccat aataggcgcg gaaggttatc tattgaaaaa
      541 acagaattct tcatttggct ttgggtttaa taaacagaac agcaaacatg gcgagcaaga
      601 gaaatccgct tgagcctgtt agtagataat tagcaagtgg aacactgcgt tgtttgggca
      661 ggttggcaga ctgggtcact tcaagtttga taaattcgtt cagattgcca gattgaattt
      721 cggtttgagt tttttcaaca aaggcgttcg cccatgctga agcaagttct tctgcttttt
      781 gcgggttttg atcatctgca taaaaatgcc agccagcttc agcaggttga gaaagggtca
      841 actttccttc acgcagttct ttaacaggag cattcaattc attcaacaca tcgtctgacc
      901 aggcaagctc caccattttg cgagcttcgc gctcaagata gtaaaagtct tgccggtctg
      961 tattggctgg aaaagcttct tcgaaattaa agtctacgtt gacaatggct ttggcacgat
     1021 acggcggtgg gacgatgtaa tagaacgcag cgccaatggc agagccaatg atcgcgccca
     1081 atacccaaaa acgccaggct ttga
//
LOCUS       DAOQJE010001003         1081 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_158764_length_1081_cov_2.050682, whole genome shotgun
            sequence.
ACCESSION   DAOQJE010001003 DAOQJE010000000
VERSION     DAOQJE010001003.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1081)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1081)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1081
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_158764_length_1081_cov_2.050682"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(88..900)
                     /locus_tag="PLT08_18715"
     CDS             complement(88..900)
                     /locus_tag="PLT08_18715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE06569.1"
                     /translation="MALLPVFLFSINGIGSSTITELLCSFGFIFGLALLPIFYGLTNK
                     LVTTQFGIENYFFYPFHVLVPWDKLIRVQIGPFGNVFLIAQQKSSKSVLNVFSQTIQL
                     DSYISNWKDNEFIQDLQQFAPQVSIPDELLHKETVRFPYRTGTFLLYFLACIVFMLVP
                     SILIPKDIIQPIKFAWGFAVFGLLGGALGGMAELLQYSVWLSSKPDETAMKKVARLLY
                     LTPFSAWLMVFIVGTLFHMATGYEVSKDMQGSVNLASLFIGFVQFKILQRVF"
BASE COUNT          361 a          215 c          218 g          287 t
ORIGIN      
        1 aaaaaggctt ggaatcgcgc cagactcccc agcgtcgggt gcacactttg ttaggcgttt
       61 tttatggatt tgaaactgac tttgcattca aaatacccgt tgcagaattt tgaactgcac
      121 aaacccaata aacaaggacg caagattaac tgaaccttgc atgtctttgc tcacttcata
      181 accagttgcc atatgaaaaa gagtaccgac gataaatacc atcagccatg cactaaaggg
      241 tgtcaagtat agtaatctgg caaccttttt catcgccgtt tcatcaggtt tgctacttaa
      301 ccatacgcta tattgtagca attccgccat tcctccaaga gcgccgccca ataatccaaa
      361 aactgcgaaa ccccatgcaa acttgattgg ttggataatg tctttcggta taaggataga
      421 agggacgagc atgaatacga tgcaagcaag aaaataaagc aaaaacgtgc ctgttcggta
      481 gggaaatcga acggtctctt tatggagaag ttcatcaggg atagatactt gaggtgcaaa
      541 ttgctgcaaa tcttgaataa actcattatc cttccagtta ctgatataag agtctaactg
      601 tatcgtttgc gaaaagacat taagaactga ctttgaagat ttttgctgtg caatcaaaaa
      661 aacatttcca aatggtccga tttggacacg aattaatttg tcccacggaa ccaaaacatg
      721 aaaaggatag aaaaagtaat tttcaatgcc gaattgcgtt gtgaccaatt tgtttgtgag
      781 accataaaat attgggagta atgccagccc aaaaataaaa ccaaatgaac ataataactc
      841 agtgattgtg cttgacccaa ttccgttaat agaaaaaagg aaaacgggta acaaagccaa
      901 aaggactcca gggattacta agagagaccg ccacgatgat tggagagaat atgttttggg
      961 gtgatacatt ttcgatgatc tcaattttat gtgttggaaa ggaaacgcct aactactgct
     1021 tatacagcca tctgtataac cccccatttg gaggtgctta accgccattt ggctgtataa
     1081 t
//
LOCUS       DAOQJE010001004         1077 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_159911_length_1077_cov_2.748532, whole genome shotgun
            sequence.
ACCESSION   DAOQJE010001004 DAOQJE010000000
VERSION     DAOQJE010001004.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1077)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1077)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1077
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_159911_length_1077_cov_2.748532"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(25..987)
                     /locus_tag="PLT08_18720"
     CDS             complement(25..987)
                     /locus_tag="PLT08_18720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE06570.1"
                     /translation="MKNTLLTFYIVFLIIAAGFGVGAATNAGILIPASVPFALVLIAL
                     LYFLRPKPELLGWAALTAGLLAGTYLVTGNVIEYVIFVVYLILTMLGIFKSPYFLAFA
                     WLFHPLWDFLPRTLPDHMHDLPLACAFFDTPIGLYLLWGSWKKRWVPFGDDSSNKAAM
                     LRSGKAIFSGILIMAVSSAIVAAVGSGYLNWVALVSSIIIIIGFRFMGETAELISWAV
                     LTGWLGMTYAHTGGTGDAIFFLVYVVISALGVFKSPYFLGIAWLVFIPYSFLPHHAHH
                     MSPDFALATIFYCLPAGMYLLWGARTKRWQAFDVNPVTEPAAVR"
BASE COUNT          365 a          267 c          231 g          214 t
ORIGIN      
        1 gaagtggttg cggtttcact ttaatcatct cactgcggca ggttctgtaa cagggttaac
       61 atcaaacgcc tgccagcgtt tcgtccgcgc accccatagt agatacatac cagcaggcag
      121 acagtaaaag attgtcgcca gcgcaaaatc aggtgacatg tgatgtgcat gatgaggcag
      181 gaaactatac ggaatgaaca cgagccatgc aatgccaaga aaataaggtg atttgaaaac
      241 tcccaaagcc gaaataacta cataaacaag gaagaatatt gcatcaccgg tcccgcctgt
      301 atgagcgtaa gtcattccca gccagcctgt aagcaccgcc catgatatca actctgcagt
      361 ctcacccatg aaacggaacc caataatgat gataattgaa gacaccaacg ccacccaatt
      421 caaatatcct gaaccaacag ccgccacaat agccgagctg acagccataa tcaaaatacc
      481 gctaaagata gctttcccgc tacggagcat cgctgcctta ttggaagagt cgtctccaaa
      541 cggtacccag cgttttttcc atgaacccca taatagatat aaaccgatgg gagtatcaaa
      601 aaaggcgcaa gccaatggca ggtcgtgcat gtgatctggc aaggtgcgtg gtaaaaagtc
      661 ccacagcggg tggaatagcc aggcaaaggc aaggaaatat ggcgacttga aaattcccag
      721 catggttaag atcaagtaga caacaaaaat gacatactca atcacattcc cagttaccaa
      781 atacgtccct gctaaaagcc cagcagttaa agccgcccaa ccaagaagct ctggtttggg
      841 gcgtagaaaa tacaacaatg caatcaaaac caaggcaaaa ggcacagatg ctggaatcaa
      901 tataccggca ttcgtagcag cacccacacc aaaaccagca gcgataatca gaaaaacaat
      961 atagaacgtc aataaagtgt tcttcatctt cttatccttt ggctaaaaat caagttcccc
     1021 gtcttgcgga cggggaactg ggagaatgcg tctggcgaag acgcgtattc aaaaatt
//
LOCUS       DAOQJE010001005         1076 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_160166_length_1076_cov_3.431929, whole genome shotgun
            sequence.
ACCESSION   DAOQJE010001005 DAOQJE010000000
VERSION     DAOQJE010001005.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1076)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1076)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1076
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_160166_length_1076_cov_3.431929"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..200
                     /locus_tag="PLT08_18725"
     CDS             <1..200
                     /locus_tag="PLT08_18725"
                     /inference="COORDINATES: protein motif:HMM:NF013543.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="inorganic phosphate transporter"
                     /protein_id="HNE06571.1"
                     /translation="IPVSTPHVISSAIVGSGAAERINKIRWSVVGEMVTTWLVTIPVT
                     GIFSCLIYFAVVEFLKIFAIL"
     gene            complement(413..904)
                     /locus_tag="PLT08_18730"
     CDS             complement(413..904)
                     /locus_tag="PLT08_18730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE06572.1"
                     /translation="MADKKCPQCGLWSTASALRCDCGYDFENGIVARLNNKLELHHYL
                     KYPAFPFLVGFGVASIHFLIAHFLLDSQLFCTTSLLSVIFLAPGFTLLLVVPDFVHSL
                     ASNMSLDFNLVMSSILYGTIGGFLGSGKIHIQAIGIVLAICFLILPICFVILIALVAG
                     GCA"
BASE COUNT          335 a          225 c          226 g          290 t
ORIGIN      
        1 gaatacctgt gagtacaccg catgttatta gttcggcaat tgtaggctca ggggcggctg
       61 agagaattaa taaaatccgc tggagtgtag tgggtgagat ggtgactact tggttagtta
      121 ctattccagt tacgggcata ttttcatgtt tgatttactt tgccgtcgtc gaattcttga
      181 aaatcttcgc tatcctgtag tagacaaaat atttttttgt tcagtccaag cagtagtcct
      241 tcttggtaac ctgcctaacg gtttgcgtta cctgcgctgg ggcggggacg gcgaacccgt
      301 ccaactggaa aaatgataag gcgcagaaaa ctgcctgaga tttgcgcaga ctccccagcg
      361 tcaggtgcac gctttgttgg gcgcttttat tgcgttgaca gataaccata gactaagcac
      421 aaccaccagc gacaagtgct attaaaatta caaaacatat aggaagaatt aaaaagcata
      481 ttgccaatac aatacctatt gcttggatgt gaatctttcc cgaacctaag aacccgccta
      541 tagtcccata taaaatgcta gacattacca gattaaaatc taaggacata tttgaagcaa
      601 gagaatgtac aaaatcaggg acgactaata aaagagtaaa accaggagcc aagaatataa
      661 cacttaataa actagtggtg cagaagagtt gggagtctaa taaaaaatga gcaattagaa
      721 agtgaatgct ggcaacccca aaacccacca ggaatgggaa agcaggatat tttaggtaat
      781 gatgtaattc taatttatta tttaaccttg cgactatacc gttttcgaag tcatatccac
      841 aatcacaacg caaagcagaa gctgtgctcc ataaaccaca ctgaggacac tttttatcag
      901 ccatttagtt tctccttgtc ctaaagcctc ctgtatttac ccttttgtat atttaccttg
      961 tggacattac acaatctaat aaagatactc aaccaataat catttgatct catcatgctt
     1021 acgccagcgc ccaacggtat gcgttacctg cgctggggcg gggacggcga agccgt
//
LOCUS       DAOQJE010001006         1067 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_162947_length_1067_cov_5.439723, whole genome shotgun
            sequence.
ACCESSION   DAOQJE010001006 DAOQJE010000000
VERSION     DAOQJE010001006.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1067)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1067)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1067
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_162947_length_1067_cov_5.439723"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            128..388
                     /locus_tag="PLT08_18735"
     CDS             128..388
                     /locus_tag="PLT08_18735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE06573.1"
                     /translation="MTLMEQIEKQIRQLPLESQREVLDFVTFLQLRMRSTLPASMEVE
                     RGKRIKAALLNLAELKVFADIKDPVEWQKQIRADRPLPGRSV"
     gene            385..753
                     /locus_tag="PLT08_18740"
     CDS             385..753
                     /locus_tag="PLT08_18740"
                     /inference="COORDINATES: protein motif:HMM:NF013970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HNE06574.1"
                     /translation="MILADSNLIIYAASGNYPALLDWFAENQPVASAVSLVETLGYYK
                     LTTKEKAALEALFAELTVLYPTVEIFQRAVDLRQQRSVSVGDALIAATALHHNLILAT
                     HNPKDFEWIESLPLVDPLAE"
BASE COUNT          274 a          226 c          258 g          309 t
ORIGIN      
        1 acggatgcgc gaggcgggga ggatggtggt gaggttcatg ggcatattat aagactgtgg
       61 ggattttgcg aaaacatttt gacagcaaaa gttgcggtta taattaattc atataggagt
      121 acactctatg actctcatgg aacagattga aaaacagatc cgccaacttc cgcttgaaag
      181 ccagcgtgag gttttggatt ttgtcacctt cctgcagttg cggatgcgtt ctacgctgcc
      241 tgcttccatg gaagtggaac gtggaaaacg tatcaaagcc gctttgctaa acctggctga
      301 gttgaaagtt tttgctgata tcaaagaccc tgttgaatgg cagaagcaga ttcgcgctga
      361 cagaccgctt ccggggcgta gtgtatgatc cttgctgata gtaatcttat tatttacgca
      421 gcatctggaa attaccccgc tcttctggat tggtttgcag aaaaccaacc tgttgcttcg
      481 gctgtgagtt tggtggaaac attgggatat tacaaactta ccaccaaaga aaaggccgcg
      541 cttgaagcct tgtttgctga attgaccgtc ctttatccca ctgttgaaat atttcagcgt
      601 gcagttgacc tgagacagca gcgttccgtt tctgtaggcg atgctctaat cgctgctact
      661 gcacttcacc acaacttgat tcttgcgacg cataacccaa aagattttga atggatcgaa
      721 tcgctgcctt tggttgatcc ccttgctgaa taattaaatc tccaattatt acttaggcag
      781 agctgagaga gcaagaaaat agatgacttg ccattcatga atttacaaat ggatggcaag
      841 tcattcgttt atttgtagat ttcattcgtg gaaggtggtc tctctccctc gatttggaca
      901 aatcgtgggt cgtttgaatg tcgggggtct tccaatattg gagtaggcgg tgtcgcttgg
      961 ctcgatacag tggcgcaaaa aatgtcagca tctccttgac ctccaaatac ggcttgtaac
     1021 tttacgccca gtattgacta gacaactttc ctaaagtaca atcgcgc
//
LOCUS       DAOQJE010001007         1067 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_163022_length_1067_cov_2.847826, whole genome shotgun
            sequence.
ACCESSION   DAOQJE010001007 DAOQJE010000000
VERSION     DAOQJE010001007.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1067)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1067)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1067
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_163022_length_1067_cov_2.847826"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(58..891)
                     /locus_tag="PLT08_18745"
     CDS             complement(58..891)
                     /locus_tag="PLT08_18745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE06575.1"
                     /translation="MPFAGDIQNNRICLGYRSGGRNSFSPVFLGKFSSINNTQTVLTG
                     ELVLHPFVKAFMVFWMSLASFSASLSVAAKIRDSLIGQKAFQIEALDGDLLLPIVGLL
                     LLLFGNTLGQSDGKAILNLLKKSLKAISVERVSSSSIFSWSNLISAGVIALFIITGTV
                     LGFSSNIRQYENPEAELTSFYTCNNSSETECTSTSYFSEKPKEIFACGHIKTTSQSSV
                     NIYWYHEPVGTPIYYNTDIWLSQGFFCEKLDIAKYDSGNYIVKVYQNRNVLGQYNFVV
                     K"
BASE COUNT          327 a          206 c          207 g          327 t
ORIGIN      
        1 ctgcttgaga ttgccgcaga atccccagcg tcaggtgcac gctttgttgg gtgcgtttta
       61 tttgacaaca aaattatact gccctaacac atttcgattt tgatacacct tgactatgta
      121 attaccagaa tcatattttg caatatcgag tttctcacag aaaaaacctt gagagagcca
      181 tatatctgta ttgtagtaaa ttggtgtgcc aacaggttca tgataccagt agatatttac
      241 gctggattgc gaggtcgttt taatgtgtcc acaggcaaag atttcttttg gtttttcact
      301 gaaataactt gtcgaagtgc attctgtttc agatgaattg ttgcaagtat aaaaggaagt
      361 taattctgct tcagggtttt catattgtcg aatgttggaa gaaaaaccta aaacagtacc
      421 agtgataata aaaagcgcta ttacacctgc tgatattaga tttgaccatg aaaaaatgct
      481 tgaacttgat actctctcaa cgcttatagc ctttaggctt tttttgagta aatttaatat
      541 tgccttaccg tcggattgtc ctaatgtgtt accaaacagt agaagtaaaa gccctacaat
      601 gggaaggagt aaatccccat ctaatgcctc tatttgaaaa gccttttgtc caattaatga
      661 atctctgatt tttgccgcaa ctgataaact cgcagaaaac gaagcaagac tcatccaaaa
      721 caccataaat gctttaacaa atggatgtaa aactaattcc cctgtcaata ctgtctgagt
      781 attatttatt gatgaaaatt tcccaagaaa gacaggagaa aacgaattac gaccaccact
      841 tctgtatccc agacaaattc gattattttg gatgtcgcca gcaaatggca agatatggtg
      901 atagagaaat acagtggaag attgcgtatt ccgcgcttct cgtatcttct ctacacattc
      961 atttatttct agtggcgatt tgaatactat tttcattata gtagaggaag cacccaacgg
     1021 tttgcgttac ctgcgctggg gcggggacgg cgaagccgtc cagccag
//
LOCUS       DAOQJE010001008         1066 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_163261_length_1066_cov_8.107814, whole genome shotgun
            sequence.
ACCESSION   DAOQJE010001008 DAOQJE010000000
VERSION     DAOQJE010001008.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1066)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1066)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1066
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_163261_length_1066_cov_8.107814"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..947)
                     /locus_tag="PLT08_18750"
     CDS             complement(<1..947)
                     /locus_tag="PLT08_18750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE06576.1"
                     /translation="MQLPAASAWISLEDCEPVYSTSTNICETKPTLVITGQEPLPNES
                     IVRVEGTYDQQAFFCDETDVCKFRIPETGDEGVKVEFWSYSSYGDSSVVYEAQVRVKQ
                     VDEGDPDQLYWYVDVLSPQWQGEVSATCAETWGVFPPVGGAPEWLSTPPQSEDLSSDI
                     PYTYLAANLILQGVVDARSCPDGGLSPGNVANQCGLEKSRDAVNEWQNQFDGLILETA
                     KKTSVPAFLLKNLFARESQFWPGIFQAVGDIGLGQLTENGADTALFWNKSFYNQFCPL
                     VMDSETCGEGYANLEEEQQVQLRRALVGSVDASCEDCPLG"
BASE COUNT          270 a          262 c          240 g          294 t
ORIGIN      
        1 ccgagcggac aatcttcaca agacgcatcc acactgccaa cgagcgcgcg tcttagctgg
       61 acctgttgct cttcctccag gttagcatac ccttcgccgc atgtttctga atccatcacc
      121 aacggacaga attgattgta gaaagattta ttccaaaaga gagccgtatc tgccccgttc
      181 tcggtgagtt gaccaagacc aatatcccca acggcttgga agatgccggg ccagaattgg
      241 ctttctcgtg caaaaagatt ctttaataaa aatgcaggga cgcttgtctt tttggctgtt
      301 tcaagaatta acccatcgaa ttgattttgc cattcattaa ctgcatcacg tgatttttca
      361 agcccgcatt gattggcaac attgccgggt gataagccgc catcggggca ggagcgagca
      421 tcaacgacgc cttgaagaat gagattcgct gcaagatatg tatatggaat atcactgctt
      481 aagtcttcac tttgtggggg ggtgctgagc cattcaggtg cgccgcctac aggtggaaag
      541 actccccatg tctcggcaca ggttgcactg acttcacctt gccattgtgg cgaaagtaca
      601 tctacatacc aataaagttg gtctgggtcg ccttcgtcca cttgtttgac acgcacttgt
      661 gcttcatata cgacactgct atccccatag gatgagtatg accaaaattc aaccttaacg
      721 ccttcatcgc ctgtttcagg gatgcggaac ttacagacat cagtctcatc gcagaagaaa
      781 gcttgctgat catacgttcc ttctacgcgg acaatcgatt cattcggaag gggttcctgc
      841 ccggtaataa caagagtagg tttggtttca caaatattgg tagatgtact atataccggt
      901 tcacaatctt caagagagat ccatgcactt gcggcaggta gttgcatgcc aatctctttt
      961 tcttttgtat ctgtatcaat aagcttcgcg taataccctt cacagtttgc aacattattg
     1021 acattgcaca tgggctgtgc catccagttg cgatagatga tggtgc
//
LOCUS       DAOQJE010001009         1063 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_164245_length_1063_cov_5.362103, whole genome shotgun
            sequence.
ACCESSION   DAOQJE010001009 DAOQJE010000000
VERSION     DAOQJE010001009.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1063)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1063)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1063
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_164245_length_1063_cov_5.362103"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(39..>1063)
                     /locus_tag="PLT08_18755"
     CDS             complement(39..>1063)
                     /locus_tag="PLT08_18755"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HNE06577.1"
                     /translation="GWRAIYIRLYTSSGLMRRVLIVGAGKAGRTLVEAYRKLNPPPFL
                     LIGYIDDDLAKKNKSYNGFAVLGDSEHLFDLIEDYRISDVVVAINGEIKGETFQTILD
                     VQERGVEVTRMPIMYEELTQRVPIEHLETDWVIRSFVDQVRVHGLYELLKRTMDILGG
                     IVGTLIFLMFLPFIAVAIILETGFPVFYSQPRLGKGAQPFTILKFRTMKQDAEMDGQP
                     KVAEENDPRVTRIGTFLRKTRLDELPQFWTVLRGEMSLVGPRAERPELVAEYQKQIPY
                     YRARLLVKPGLTGWAQINYGYVATVKETYIKLEYDLYYIKHRTLNMDFSIVLRTIGTV
                     LRRTGR"
BASE COUNT          304 a          257 c          219 g          283 t
ORIGIN      
        1 ataaagcccg caccgccagt gactaaatat ttcataggtt atctcccagt tcttcttaat
       61 actgttccga ttgtccttaa cacaatacta aaatccatgt ttagggttcg gtgtttaatg
      121 tagtatagat cgtactcaag cttgatatag gtctctttga ccgttgcaac atatccataa
      181 ttaatctgcg cccatcctgt aagtccaggc tttaccaata atcttgcacg ataatacggt
      241 atctgtttct gatactctgc aactaattca gggcgctcag cacgcggacc aactagactc
      301 atttcgccac gcagcacagt ccaaaattgc ggaagttcat caaggcgagt ctttcgaagg
      361 aaagtgccaa ttcgggtaac acgtgggtca ttctcttcag ccactttagg ttgcccgtcc
      421 atttctgcat cttgtttcat ggtgcggaat ttaagaatag taaaaggctg tgcacctttt
      481 cctaaacgcg gctgagaata aaaaacaggg aaacctgttt caagaataat ggcaacagca
      541 ataaagggga gaaacatcaa aaagatgagc gttccaacaa tgccacccaa aatatccatt
      601 gtgcgtttta gcaattcata cagtccatgc acgcgcactt ggtcgacaaa tgagcgaatc
      661 acccaatcgg tttcaagatg ttcgatcggc actcgttgag tcaattcttc atacataatg
      721 ggcatgcgag ttacttcgac acctcgctct tgcacatcaa gaatggtttg aaaggtttcg
      781 cctttgatct caccgttaat cgccacaaca acatctgaaa tgcgataatc ttcgatcagg
      841 tcgaaaagat gttcgctatc gccaagcaca gcaaacccgt tatatgattt attctttttt
      901 gcgaggtcgt cgtcaatata gccaatcaaa agaaagggtg gcggattcag tttgcgatac
      961 gcctctacca aagtccgccc ggctttacct gcaccaacaa taagcacccg gcgcatcaaa
     1021 ccggatgagg tgtagagtcg gatgtagatg gcgcgccagc cca
//
LOCUS       DAOQJE010001010         1062 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_164597_length_1062_cov_8.873883, whole genome shotgun
            sequence.
ACCESSION   DAOQJE010001010 DAOQJE010000000
VERSION     DAOQJE010001010.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1062)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1062)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1062
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_164597_length_1062_cov_8.873883"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..272)
                     /locus_tag="PLT08_18760"
     CDS             complement(<1..272)
                     /locus_tag="PLT08_18760"
                     /inference="COORDINATES: protein motif:HMM:NF012576.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HNE06578.1"
                     /translation="MANKKKSPTIYDVAELSGVSISTISRVLNSPDKVNTETRQQVMS
                     AIDQLGFVPKAEARARAMQNTNRIGVITPFFTAPSFVQRLRGVASRL"
     gene            446..>1062
                     /locus_tag="PLT08_18765"
     CDS             446..>1062
                     /locus_tag="PLT08_18765"
                     /inference="COORDINATES: protein motif:HMM:NF012742.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HNE06579.1"
                     /translation="MRTKKRAVTIQDVAKAAGVSVSTVSRVLNGKTDVAGETQERILK
                     IINKLGFTSNLAARSMRSLKKNLIGLVVPDIHPYSVEIMKGVNKAIAESNYDLLVYTT
                     GDALKHGATLHEQHYISLLSNSITDGVIIVASATAEFTAEAPIVAVDPHVPNPKYPSV
                     QGMNYHGALDAMEYLFKLGHQRIGFMSGREGNVSGDRRFQGYKNA"
BASE COUNT          293 a          229 c          259 g          281 t
ORIGIN      
        1 agtctgcttg ccacgccgcg caagcgttgg acgaaggatg gggcggtaaa aaatggggtg
       61 atgaccccga tgcggttggt gttctgcatg gcacgcgcac gcgcctcagc cttcgggacg
      121 aagcccagct ggtcaatggc agacataacc tgttgacggg tttcggtatt aactttatct
      181 ggtgagttta gcacccgcga aatggtggaa atgcttacgc cagaaagttc tgcaacatca
      241 taaatagtag gggatttttt cttatttgcc atattgtatg tgaaagctaa gtgaaagcac
      301 tttcagttta acatgaaaca gtctcaaatt ccagattctt agagtttcaa ttaagtagtt
      361 gactttttat tttttttatg caataatacc ggtaacgttt ccggtattga taccgaaaaa
      421 tagagcattt tcaccaggaa ttggtatgcg aactaaaaaa cgcgcagtga caattcaaga
      481 tgttgcaaaa gcagcaggtg tttctgtatc aaccgtttcg cgcgtattaa acggtaaaac
      541 ggacgttgcg ggggaaacac aagaacgtat tcttaagatt atcaataaac tcggtttcac
      601 gtccaatcta gcggctcgca gcatgcgcag cctcaaaaag aatttgatcg ggttggtcgt
      661 tcctgatatt cacccctact ccgttgagat catgaagggt gttaacaagg cgatcgcaga
      721 gtcaaattac gaccttttgg tatacaccac gggtgatgcg ttgaaacatg gggcaacctt
      781 acacgagcaa cactatattt ctctgcttag caactccatt accgatgggg taatcattgt
      841 ggcatctgcg actgccgaat tcactgccga agctcccatt gtggcagtgg acccgcatgt
      901 gcccaaccca aagtatcctt cggttcaagg tatgaactat cacggcgctt tggatgccat
      961 ggaatatctc ttcaaattag gacatcaacg aatcggattc atgagcgggc gcgaaggcaa
     1021 tgtcagtgga gataggcgct tccagggata taaaaatgcc tt
//
LOCUS       DAOQJE010001011         1042 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_171478_length_1042_cov_4.302938, whole genome shotgun
            sequence.
ACCESSION   DAOQJE010001011 DAOQJE010000000
VERSION     DAOQJE010001011.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1042)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1042)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1042
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_171478_length_1042_cov_4.302938"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..372)
                     /locus_tag="PLT08_18770"
     CDS             complement(<1..372)
                     /locus_tag="PLT08_18770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE06580.1"
                     /translation="MLNKIFSDPKRIVILIIIAIIVLFVCAVVLIGGGWSILHRSDLT
                     PTATPISFGYCGAEFTSLCVVSFGRDALGNTVINLYVPIREYPVFYLKVVRQSGEQRF
                     ECEWNRDVKESVYCTGSPLNLG"
     gene            515..1024
                     /locus_tag="PLT08_18775"
     CDS             515..1024
                     /locus_tag="PLT08_18775"
                     /inference="COORDINATES: protein motif:HMM:NF024698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNE06581.1"
                     /translation="MDLFETQRLLIRHLEMTDVDAMFRIFSLPEVTKWMGDGEPLSQE
                     LCGKWIEISQKNYATKGFGASAVIEKASGEFIGCSGIVYDSERQEPEIIYAFHPSAWG
                     KGYASELVPAMLKYGLTECGLTYILATIAEANKASAHIVEKAGMQFVAEEPNPDGHIT
                     LVYRIEKGN"
BASE COUNT          335 a          227 c          204 g          276 t
ORIGIN      
        1 gcctaaattc aatggagagc ccgtgcaata cacactttct tttacatcac ggttccattc
       61 gcattcaaat ctttgttctc ctgattgtct aaccactttc aagtaaaaga ctgggtattc
      121 tcttattggt acatataagt taatgactgt attacctaat gcatcgcgtc caaatgacac
      181 cacacataaa cttgtgaatt ctgcaccaca atagccaaaa gatataggag tggcagttgg
      241 agtaaggtca cttcgatgca atattgacca ccctccgcca attaaaacaa cagcacaaac
      301 aaacagaact atgattgcaa taattattaa tatcacaata cgttttgggt ctgaaaagat
      361 tttattcagc attatttcca ttctaccatc gcaaaattct atatattaag tgtatataat
      421 gcaacttcag gaacaaaatc cgtctcagaa ccgttctaga gaatatttcc agttcaactg
      481 gaatatgata tgttgtgaat tttaggagca aaaaatggac ttatttgaaa cgcagcgttt
      541 attgattcgg catcttgaaa tgacagatgt tgatgccatg tttagaatct tcagcctgcc
      601 tgaagtaaca aaatggatgg gagatggaga acctctcagc caagaactct gtggaaagtg
      661 gatcgaaatc tcacagaaaa actatgcaac caaaggcttt ggcgcttcag ctgtgattga
      721 aaaagcctca ggtgagttca tcggttgcag cggcattgtg tatgactcag agcggcagga
      781 accagagatc atttatgcct ttcatcccag cgcatggggc aaaggctacg ccagcgaact
      841 tgtccctgcc atgttaaagt acggtctgac cgaatgcgga ctgacttata tccttgccac
      901 catcgcagaa gctaataaag cctcagcaca catcgtagaa aaagcaggca tgcaattcgt
      961 cgccgaagag ccaaaccctg atggtcacat cacccttgtc tatcgtatcg aaaaaggaaa
     1021 ctaaaatgcc aagcgttgaa ac
//
LOCUS       DAOQJE010001012         1038 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_173124_length_1038_cov_2.208545, whole genome shotgun
            sequence.
ACCESSION   DAOQJE010001012 DAOQJE010000000
VERSION     DAOQJE010001012.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1038)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1038)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1038
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_173124_length_1038_cov_2.208545"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            274..>1038
                     /locus_tag="PLT08_18780"
     CDS             274..>1038
                     /locus_tag="PLT08_18780"
                     /inference="COORDINATES: protein motif:HMM:NF013125.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lamin tail domain-containing protein"
                     /protein_id="HNE06582.1"
                     /translation="MPKQTRFSRLALILMLLCALCVGTFSPRVQQVEAKPVNAADELN
                     VFISEFRVRGPNGADDEFVEIFNATGGSISLNGWKLRKSSGCGSTLADLTTIGAINIA
                     PGQYYLIAKSGSYSGSTTADLLYTTTGISDDGGIALVDATNKIIDQVGLCADTTYKEG
                     TTISSMSGTANQSYERKSGNSAGSCFDTDNNSTDFIFNSSTSNPQNFSSAAIPCLSVT
                     SVASTIVSPVTYSDISGTVIDIQLTFSNVVTVTGSPT"
BASE COUNT          264 a          239 c          237 g          298 t
ORIGIN      
        1 tggatgttga gatcgaggat aaggttcgaa ccgccattaa gttggtgatc gagccgcgca
       61 atcctgctcc agccacttcg taacacctgg gtgacagtct ttttatgggc tgtcacctat
      121 tcttttttat ttgcggatgt agggcggtct gtatatacct gccaagggta cttgtgccct
      181 gccaagggta taatttctgc acgtggtttc gcgcttcaga cttaactgta agtgggttct
      241 ccccatgaac cgattccacg ttgattcctc ctcatgccca agcagacccg tttctcgcgc
      301 cttgcactca tcctaatgct tctctgcgcc ttgtgcgtag ggactttttc tccacgtgtg
      361 cagcaagtgg aggcgaagcc agtaaatgcc gcggatgagt tgaatgtatt tatcagtgaa
      421 ttccgtgtgc gcggacctaa tggcgcagat gatgaatttg tagagatatt caatgccaca
      481 ggcggaagta tttcactcaa tggttggaaa cttagaaaat caagtggctg tggctcaacc
      541 ttagcagacc ttacaacgat cggagccatt aatattgctc cagggcaata ttatcttatt
      601 gccaaatcgg gaagttattc aggatctaca acagcggatc ttttgtatac cacgactggt
      661 atttctgatg atggcgggat tgctcttgtt gatgcaacaa acaagataat tgaccaagta
      721 gggttatgcg ccgacacaac ttataaagaa ggcactacta tatcttctat gagtggaact
      781 gccaaccaaa gctatgaacg caaatcaggc aacagtgcgg gtagttgttt cgatacagac
      841 aataattcta cagattttat tttcaattca tccacaagta acccgcaaaa cttttcaagt
      901 gcggctattc cttgtttgtc cgtaacgagt gttgcttcaa cgattgtcag ccctgtcact
      961 tattccgaca taagcggaac agtcattgat attcaattga cctttagcaa tgtcgtcacc
     1021 gtgacaggct cgccaacc
//
LOCUS       DAOQJE010001013         1033 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_174772_length_1033_cov_4.548057, whole genome shotgun
            sequence.
ACCESSION   DAOQJE010001013 DAOQJE010000000
VERSION     DAOQJE010001013.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1033)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1033)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1033
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_174772_length_1033_cov_4.548057"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            133..957
                     /locus_tag="PLT08_18785"
     CDS             133..957
                     /locus_tag="PLT08_18785"
                     /inference="COORDINATES: protein motif:HMM:NF025056.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003953 - NAD+ nucleosidase activity
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="toll/interleukin-1 receptor domain-containing
                     protein"
                     /protein_id="HNE06583.1"
                     /translation="MIKIFLSYAHEDYSSAKRLYDTFNSIPSVNVWFDKESLLPGRKW
                     EIEIRKEIRASQFFLLLISSNSTEKKGFYQREIREALKVLEEYPDDKIFLIPVRLDDC
                     QPHFEQLTALQYVDLFPESEWFIGINKLMKVFQSHQQPASSSNNSTTVRFRTHQAVFR
                     NSPVLFYFLTITNLHHQPIEITHIWYQDPNHHIAIQYQSRPLPKRLNVNEGWETWLAI
                     DHIPEEFRNNAYDSFRIRLSTGEVFASSKDDTTPPIGTIPGGGIYPSDIDGFQEQS"
BASE COUNT          339 a          216 c          190 g          288 t
ORIGIN      
        1 tttctggttg gacggcttcg ccgtccccgc cccagcgcag gtaacgcaaa ccgttcggcg
       61 gcttcgcaga aaatgcagta gtcttaatca aaactcttaa gtgtgtaata taaggtcgga
      121 ggttatcata gtatgataaa aatcttttta agttacgcac acgaagatta ttcatctgct
      181 aaaaggcttt acgatacatt taactcgata ccatcagtca atgtatggtt tgacaaagaa
      241 tctctattgc ctgggcgaaa atgggagata gaaattcgca aggaaattcg ggcttctcaa
      301 tttttcttac tgctcatttc cagtaattcc accgaaaaaa aaggttttta tcaaagagaa
      361 ataagagaag cactgaaagt tttagaggaa tatccagacg acaagatatt ccttatacct
      421 gtacgcttgg acgactgcca accccacttt gagcaattaa ctgctttgca atatgttgat
      481 ttatttcctg aatcggaatg gtttattgga ataaacaaat taatgaaggt ttttcaatca
      541 catcagcaac cagcatcaag cagtaacaat tccactacag tcaggtttag aacacatcaa
      601 gcagtttttc gcaatagccc cgttctattt tattttctca caattacaaa tttacatcac
      661 caacctattg aaataacgca tatatggtat caagacccaa atcatcatat tgccattcag
      721 tatcaatctc gcccactacc caaaaggcta aatgtaaatg aaggatggga aacttggtta
      781 gcaatagacc atatacctga agaatttaga aataatgctt atgactcttt tcgaattcga
      841 ttgtcaactg gagaagtatt cgcatcgtca aaggatgata caacaccgcc gattggcaca
      901 atacctggag gaggaattta cccttcagac attgatggct ttcaagaaca aagttaatat
      961 tacaatcaag agccgccgaa caaagcgtgc acctgacgct ggggattctg cgcaaatctc
     1021 aagcagtttc cta
//
LOCUS       DAOQJE010001014         1029 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_176319_length_1029_cov_2.740246, whole genome shotgun
            sequence.
ACCESSION   DAOQJE010001014 DAOQJE010000000
VERSION     DAOQJE010001014.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1029)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1029)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1029
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_176319_length_1029_cov_2.740246"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1029)
                     /locus_tag="PLT08_18790"
     CDS             complement(<1..>1029)
                     /locus_tag="PLT08_18790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE06584.1"
                     /translation="VFIALWFVAGWLWMNTPASPNYFLPDTANNVLYPYSDGATFDIG
                     GQFALIGQGFFNGIFFDRMLYSSFLAYLHLFFGQDYLVLLTAQAAVYAVFPAVVYLLG
                     KELHSRALGITAGILIALRGLTAIVASKWIDVASPKMMLTDFPTAIGISVFLFVVMKW
                     WQQPTKTKLLIWAGAVFGLVVMIRSHVITLLPIILFFVLLRLGWSWQRVVLVALLILI
                     GSFSISLPWEIRNQSHGIPMFYIYYSRIELILRYRYGIGAYTSPTHESVAEVAPRQRF
                     NREKESDECETVPCSITNHFVHNIITSFVSLPASLTFEDTFNIVKSDLPYWNKDWRDG
                     QIDIGGWVG"
BASE COUNT          363 a          236 c          189 g          241 t
ORIGIN      
        1 ccccacccat ccgccaatat ctatctgtcc atcacgccag tctttgttcc aataaggcaa
       61 gtctgatttc acaatattga atgtatcttc aaaggtcaat gaggcgggta gtgagacaaa
      121 ggaagtaata atattatgaa caaagtgatt ggttatagaa cagggtaccg tttcacattc
      181 atcactttcc ttctcgcgat taaatctttg gcgcggtgct acctctgcaa cagattcatg
      241 ggtaggcgaa gtataagcgc caatcccata acgataacgc agtatcagtt caatgcgaga
      301 ataatatata tagaacatag gaataccatg tgattgattc cgtatttccc aaggcagaga
      361 tatactgaaa gaccctatta ggattaataa agccaccaag acaactctct gccaactcca
      421 tcccagtcgc aataaaacaa aaaacagaat aattggcaac agagtaataa cgtgagagcg
      481 gatcatgaca accaaaccaa agacagcacc tgcccagatc aatagttttg tttttgtagg
      541 ctgttgccac cacttcataa caacgaaaag aaatacagaa attccgatag cagtcgggaa
      601 atcggtcaac atcattttcg ggctggcaac atcaatccac tttgaggcaa caatagcagt
      661 caacccgcgt aaggcaatca atattcctgc cgtaataccc aacgcccgac tatgtaattc
      721 cttccctaac aaatacacca ctgctggaaa aaccgcatac actgccgctt gagcagtgag
      781 tagaactaag taatcttgtc cgaagaataa atgtaaatat gctaaaaagg aactataaag
      841 catacggtca aaaaagatgc cattgaagaa tccctgcccg atcaatgcaa attgtccacc
      901 tatatcaaag gtcgcgccat cagaatatgg ataaagaaca ttattggctg tgtctggtaa
      961 aaaataattt ggactagctg gcgtattcat ccacaaccaa ccagcaacaa accaaagtgc
     1021 gataaaaac
//
LOCUS       DAOQJE010001015         1026 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_177539_length_1026_cov_2.021627, whole genome shotgun
            sequence.
ACCESSION   DAOQJE010001015 DAOQJE010000000
VERSION     DAOQJE010001015.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1026)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1026)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1026
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_177539_length_1026_cov_2.021627"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(76..903)
                     /locus_tag="PLT08_18795"
     CDS             complement(76..903)
                     /locus_tag="PLT08_18795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE06585.1"
                     /translation="MDKKSELKVIGLYRAEFELAKSSELKSWLCQLLMASTALLSIFT
                     NSEFIVYPSTILALLAAFFDKWFSYQSKQQKDNAERARRLLLMVNGLGYQVSKKELAD
                     LFANFSVSKTSGEKWESKDYFQSTEKIGYIRLSQLLQESAFFSKYLYAESAKLSWVLF
                     SVFFIISLTTLFILPILPSPSWSITIAKIIGIVLMFLVSIDLFGRALEYMDATNSTRH
                     TDDRLENMRSGNINEHDILLIFGDYNTTVAGAPLIPTFIYNWNKARLAELWKKRLSR"
BASE COUNT          317 a          191 c          194 g          324 t
ORIGIN      
        1 tgggatgtgc gcagaatccc cagcgtcagg tgcacgcttt gttgggcagt ttttggggta
       61 gcacgacaac atggtttaac gagataaccg ttttttccaa agttctgcca atcgggcttt
      121 gttccaattg taaatgaaag ttggaatcaa aggtgcgccc gcaacagttg tgttgtagtc
      181 accaaaaatc aataaaatat catgttcgtt tatattacca ctacgcatat tttcaagcct
      241 gtcatcagtg tggcgggttg aatttgtagc gtccatgtac tccaatgctc tgccaaataa
      301 atctattgag acaagaaaca ttagaacaat accaataatt ttggctattg tgattgacca
      361 agagggacta ggcaaaatag gaagtataaa aagtgttgtc aaagaaatta tgaaaaatac
      421 gctaaacaga acccaactta attttgcgct ttcggcataa aggtatttag aaaagaaagc
      481 actttcttga agcaattgag ataaacggat atatccaatt ttttctgttg attgaaagta
      541 atccttgctt tcccattttt ccccagatgt ttttgagaca gaaaaattgg caaaaaggtc
      601 tgcaagttct ttttttgaga cttgataccc aagcccatta accataagta ataagcggcg
      661 cgctcgctca gcattatctt tctgctgttt tgattgatat gaaaaccatt tgtcaaaaaa
      721 agccgccaat aatgcgagga tggttgaagg ataaactata aactccgagt ttgtaaaaat
      781 tgacaataat gccgtagatg ccataagcaa ctggcataac caagatttca actcgctaga
      841 ttttgcgagt tcaaattctg ctcgatataa accaatcact tttaattcgg actttttatc
      901 cataggatgg aaactcgttt ccgaaaattt gtttccaaat ttttattgcg ccttcattgt
      961 cagaattttt atcataagtt ttataccatg cgtctcttgc gctatctctt gctgtttcta
     1021 atcgtt
//
LOCUS       DAOQJE010001016         1024 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_178052_length_1024_cov_5.464396, whole genome shotgun
            sequence.
ACCESSION   DAOQJE010001016 DAOQJE010000000
VERSION     DAOQJE010001016.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1024)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1024)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1024
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_178052_length_1024_cov_5.464396"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..732)
                     /locus_tag="PLT08_18800"
     CDS             complement(<1..732)
                     /locus_tag="PLT08_18800"
                     /inference="COORDINATES: protein motif:HMM:NF014571.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAC(3) family N-acetyltransferase"
                     /protein_id="HNE06586.1"
                     /translation="MLSKKELLDGFGQLNLKKGETIIVHTSYKSLGGVEGGADTVIDV
                     MSELVGKDGTVLFPAFNFQSWTESHYFDVKETPSKMGAITEQARLRPNVLRTPHPIYS
                     FSALGARAEEFSKTEDVEAYGPNSAFALFHKINGTIVSIGLDFNSTFSMHHYIEYNVG
                     CNYRRIKEFSGIYVGYDRVPKLKMHSMFVRNDDTVKTYINPGMNELLEAGVIKEVQVG
                     AAKIHFATANDFYDNMSVIVREHPEK"
     gene            complement(841..>1024)
                     /locus_tag="PLT08_18805"
     CDS             complement(841..>1024)
                     /locus_tag="PLT08_18805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE06587.1"
                     /translation="QNGWLFRDGDVDHLAEKILAAMNQREKLPEIGRASRSEAEKRAD
                     WKKNAAALMEVYRSLK"
BASE COUNT          258 a          289 c          201 g          276 t
ORIGIN      
        1 tttctcagga tgttcacgca caatgaccga catattatcg tagaaatcat ttgccgtcgc
       61 aaagtggatc ttcgccgcac cgacctgcac ttctttgatc acgcccgcct ctaacaactc
      121 attcatacca gggttgatat aagtcttcac tgtatcatcg ttacgaacga acatcgagtg
      181 cattttaagt ttcggcacac ggtcatagcc aacataaatg ccagaaaatt ctttgatgcg
      241 gcgataatta cagccaacgt tatattcgat gtaatgatgc atcgagaaag tgctattgaa
      301 atccaagcca atacttacaa tggttccgtt gatcttatgg aacaatgcaa acgccgaatt
      361 cggaccgtag gcttccacat cttcggtctt tgaaaactct tccgctcgcg cacccaaggc
      421 agaaaaacta tagatagggt ggggagtccg caacacattc gggcgcaaac gtgcttgctc
      481 ggtgatcgca cccatcttcg acggcgtttc tttcacatcg aaataatgac tctccgtcca
      541 cgattggaag ttgaacgccg ggaacagcac cgtgccatcc ttgcccacca attcactcat
      601 cacatcaatg accgtgtccg cgccgccttc caccccgccc aacgatttat acgacgtatg
      661 cacaatgatc gtctcgcctt ttttcaagtt gagttgacca aaaccatcca ataattcttt
      721 tttacttaac atattttcct tttcccgtca ttgcgaggag ggcgacagct tgacgaagca
      781 atcttgtact caattatgag attgcttcgg gcgaaagaac atcgccctcg caatgacgta
      841 tcattttaga cttcgataga cttccatcaa cgccgcggca ttctttttcc aatccgcgcg
      901 cttctctgct tcacttcggc ttgctctgcc gatctcaggc agtttctcac gctgattcat
      961 tgccgccaga atcttctctg caaggtgatc cacatcccca tcgcggaaca gccagccgtt
     1021 ctga
//
LOCUS       DAOQJE010001017         1019 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0608
            NODE_179894_length_1019_cov_4.272822, whole genome shotgun
            sequence.
ACCESSION   DAOQJE010001017 DAOQJE010000000
VERSION     DAOQJE010001017.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810240
            Sequence Read Archive: SRR3501858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1019)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1019)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:48:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,762
            CDSs (total)                      :: 3,740
            Genes (coding)                    :: 3,721
            CDSs (with protein)               :: 3,721
            Genes (RNA)                       :: 22
            tRNAs                             :: 22
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 2 of 19
            Pseudo Genes (incomplete)         :: 17 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1019
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_179894_length_1019_cov_4.272822"
                     /isolate="AMR_MDS_0608"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            106..891
                     /locus_tag="PLT08_18810"
     CDS             106..891
                     /locus_tag="PLT08_18810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNE06588.1"
                     /translation="MTSNEIVEISTPHVKRFLFGSLIVIALDGAIFLTQFFPSLQLPF
                     ILLIPLILIAGIAQMFVIYRQSQSELPLIPFTIGVIFIIGGAVFDMVVTVIVSSDLKL
                     EANPIARLLLDSGYSIVLVYSYGLLAQFLLVALYCVMWASFLRHCDVITAPANSMSIS
                     FLDYFKATHGGDHLSWRQFMFPVKMSELPKIYYMIWFFVVVVVGGASQVRWYLGLEWL
                     NVAVGYRTQIAIFSSVLFMVGYEVWLFICYQSAQRNISKIKTG"
BASE COUNT          247 a          206 c          218 g          348 t
ORIGIN      
        1 cggctttttc ctactctcaa acagaatcca cgcccgccca cacgcaggta acgcaagccg
       61 ttagcccgtt ccttgcattc attattattt ctaaagggat attagatgac ttcaaatgag
      121 atagttgaaa tatctacacc acacgtcaag agattccttt tcgggtcact tatagttatt
      181 gcacttgatg gtgcaatttt cttgactcaa tttttcccgt cacttcaact cccgtttatc
      241 ttattaattc cactcatatt aattgctgga attgcccaga tgtttgttat atatcgtcag
      301 agtcaaagtg aacttccatt aatacctttt acgattgggg ttattttcat cattggcggc
      361 gcagtctttg atatggttgt tacggtaata gttagttcag atttaaaact tgaggctaat
      421 ccaattgcta ggctactatt ggattctgga tattcaatag ttctcgtata tagttacgga
      481 ttactggcgc aatttctttt agttgcattg tattgcgtga tgtgggcttc cttcctgcgt
      541 cactgcgatg taattacagc gccagcaaat tctatgagca tttcttttct tgattatttc
      601 aaggctacgc atggtggcga ccatttatcg tggcggcaat tcatgttccc agtaaaaatg
      661 tcggagttgc caaaaatata ttatatgatt tggtttttcg ttgtagttgt ggtgggaggt
      721 gcaagtcagg ttcgatggta tttgggacta gaatggctta atgtggcagt tggttacaga
      781 acccaaattg cgatattctc aagtgtgttg tttatggttg gatatgaagt ttggctattt
      841 atttgttatc aatctgctca aagaaatatt tcaaaaatca aaacgggcta acaaagcgtg
      901 cacctgacgc tggggagtct gcggtcatcc caagcagttt cctacgcctt atcatttttc
      961 tgcttggacg gcttcgccgt ccccgcccca gcgcaggtaa cgcatgccgt tgggcagtt
//
